LOCUS       DAOTBO010000001        85504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_208_length_85504_cov_5.709792, whole genome shotgun sequence.
ACCESSION   DAOTBO010000001 DAOTBO010000000
VERSION     DAOTBO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 85504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85504
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208_length_85504_cov_5.709792"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1056)
                     /locus_tag="PLE88_00005"
     CDS             complement(163..1056)
                     /locus_tag="PLE88_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM74881.1"
                     /translation="MASHHLAKLKIDDLEIIGYSVAGEETVVGMPQLDVCFDIGKAPG
                     QLISINHVLLTHGHMDHAAGIAYYLSHRKFCGLPEGTVLTPPNTIESIRQILDAWGRL
                     DGTRIPANLVPVQPGDEYRLKPNLVARVFPTKHTRQAVGFSVIETRKKLKPEYHHLTG
                     PQLVELKKQGVVIDNPVEVPIVSYLGDTSYVDFSQLDYVSNSRILIAECTFFADEHTQ
                     RADAGRHMHIDEFGPLLERMNNQVILITHLTQRTSMIEARKLLREKLPAEIFKKVIFL
                     MDRKYRQHAGKKDNSNIKQEA"
     gene            1161..1910
                     /locus_tag="PLE88_00010"
     CDS             1161..1910
                     /locus_tag="PLE88_00010"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOM74882.1"
                     /translation="MPDKRSHRGPHPDDAALFTDQSQFILAKATADMSWLLSRGYAEK
                     SSLKLIGDRYNLKQRQRIAVMRSACSDAQLHRRRATEFKQEKIKGQPLILDGYNLLIT
                     IEAALGGGILLIGRDGCIRDLASIHGTYRKVEETLPAIGIIADTLNRLEITNVTWLLD
                     RPVSNSGRLKNILMQFAQSRMVNWTVELVQSPDKTLIESGWIIATSDSAVLDSCLQWL
                     NLGKIAIESLKPNHRLRLFDMRMPESSSDTI"
     gene            2093..2689
                     /locus_tag="PLE88_00015"
     CDS             2093..2689
                     /locus_tag="PLE88_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM74883.1"
                     /translation="MAVAQETSSASLIEAARTDKMAFSTLFRRYYDEIFRYCARRLPD
                     RDNAEDAASQVFLKMVSNFDSFTGDEVAFRCWLFRIACNEINNFYRQTGRQNRLFEKL
                     QHETACSNATSTGFSDNENQAKLQFLRAAVSTLKPNYQDILTLRFFQGLTSEQIAEIL
                     GLNPATVRSRLSRSLKHLKKLYRLHQQKIAKEIFSDDA"
     gene            2679..3272
                     /locus_tag="PLE88_00020"
     CDS             2679..3272
                     /locus_tag="PLE88_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74884.1"
                     /translation="MTPDEHNFEEFVKTIRFDDQPSSEHRQRLEFQLLQRWENRQKET
                     GYIEPAALYLKRLAIAAGILLASSVLFWTIDNLWISRPLAVHPDSKMIETILQQEQPS
                     EAEKPHLLAQINHVWHLICRQDSEALVSILQTQETAYAIRCWAAKYLGQFGNEKTLEL
                     LNTAIETLHISDSNEPLVLAARTIRQRLEASEPNTPN"
     gene            complement(3401..3712)
                     /locus_tag="PLE88_00025"
     CDS             complement(3401..3712)
                     /locus_tag="PLE88_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74885.1"
                     /translation="MGRWRSKILFTLIVYCSGFLTAVYFLAPVSAAALSETQSEGQHS
                     RLPAEEAFGGNAGIDCHAWASSIRSGIDICIRFAEENALRAADLFRSKMGQSSQSDRH
                     Q"
     gene            complement(3991..5115)
                     /locus_tag="PLE88_00030"
     CDS             complement(3991..5115)
                     /locus_tag="PLE88_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOM74886.1"
                     /translation="MEIKVPLSRPDISQAEIDAVCAVLRSPYLSLGPKLREFEQAFER
                     YIGLKHAIAVNSGTSGLFLCTQALGWGPGDEIITTPFTFIATVNIILMAGAKPVFVDI
                     DPETLNIDADKIEAAITEKTKGIVPVEVFGSPAGMDCICAAAQKYKLTVLEDSCEGLG
                     SQLNGKKVGTFGKMSVFAFYPNKQMTTGEGGMILTDDDELASACRSLRNQGRGTGPGW
                     LAHERLGYNFRLCDINCALGIVQLSRIEEFVRKRSEVADMYRHYLAGDDRVIVPQTPA
                     GCRMSWFVFVIRLADRYTRQQRNSVLEKLTARGIQVGNYFPPVYLQPFIAEKFGFKEG
                     DFPVTDTISSSTIALPFHNNLTEKEIQQVCRELKACLDEL"
     gene            5283..6113
                     /locus_tag="PLE88_00035"
     CDS             5283..6113
                     /locus_tag="PLE88_00035"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOM74887.1"
                     /translation="MKTQRLTIRTFFGIITAGLIVSGFSSCREDSSLSGPSGYPPATE
                     KSLSKSAEPNQPQVWRRPRSDERRQERLEMVQWIRRGYGLDEPRILEAMQNVPRHWFV
                     PPAQQPYAYHDTPLPIGEGQTISQPFIVAAMTHLLALNFNDRVLEIGTGSGYQAAVLN
                     EFTPQVFTIEIIESLGKRAIQTFREHGYDTICVKIGDGYKGWPEYAPFDAIIVTCAPD
                     SIPPPLLDQLKAGGRMVIPVSRETGYQELILVEKDAQGSVSKRSVMPVRFVPLIRKGN
                     "
     gene            complement(6230..8896)
                     /locus_tag="PLE88_00040"
     CDS             complement(6230..8896)
                     /locus_tag="PLE88_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOM74888.1"
                     /translation="MNRQRIVWILMIAEGICWAADSAKPVYQDVTVDFEIRASDLVER
                     MTLEEKISQLGDAAPAIERLGIPPYNWWNECLHGVARAGTATVFPQAIGMAASFDRAM
                     MYKTASIISDEARAKHHEFVRQQDYGRYKGLTFWSPNINIFRDPRWGRGHETYGEDPY
                     LTGQMGIQFVRGLQGEHPRYLKVVATAKHFAVHSGPEADRHHFNAVVNTRDLWETYLP
                     AFYDLVATAKAYSVMGAYNRVDSESASASWMLLQDILRQQWKFDGYVVSDCGAIQDIY
                     ANHKIAQNAAQAAAIGLRRGCDLECGQVYQRWLLDAVQAGMMDEGQIDRAVWRLMLAR
                     MKLGMFDPPEMVPYAQIPYSVNDCPAHSQAALEMARKSMTLLKNTGILPLDKKRIKTI
                     AVVGPNADSTTVLRGNYYGQASHPVTVLEGLWKAAGNEVEILYWLGCPLAEGVERTAY
                     QPVDSRYLFCRDEAGREVSGLRGDYYSGIHLEGKPVLTRIDTVINMDWGLDSPTATAV
                     AQGILAPDKQMEADNFSVRWTGQLQPPVSGVYKLGIMSDDGCRLYIGGKKVIDGWSEP
                     AKQPYWADVSLEKDQRCDITIEYYEASKDAGIHFVWLPPDSSAPSGRMLEDVAKADVA
                     IFVGGLDADIEGEEMKLQADGFNRGDRTKIELPAVQLETIKAMQQTGTPVILVLMAGS
                     AIAFDGLDQTLPAILMAWYPGQRGGDAVAEVLFGDYNPAGRLPVTFYASTQELGDFSD
                     YAMAAGKGRTYRYYTGQPLYPFGHGLSYTEFAYSELKLSRDEAGPEETITVSAVVKNT
                     GNRDGEEVVQLYVRDVQSRWPMPLKQLRGFERISLAKGQQKTVEFVLKPSIDMRYYDS
                     RLSRYAVEPGEFEIQIGSSSGDIRLKKAVLVR"
     gene            9144..11690
                     /gene="leuS"
                     /locus_tag="PLE88_00045"
     CDS             9144..11690
                     /gene="leuS"
                     /locus_tag="PLE88_00045"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOM74889.1"
                     /translation="MENGQYDFNRIEKKWQNYWLVNKTFKTPDTSAKPKYYVLDMFPY
                     PSGAGLHVGHPEGYTASDIVARYKRMRGFNVLHPMGWDAFGLPAEQYAVQTGTHPAVT
                     TQKNIDNMRRQIQSLGFSYDWDREVNTTDIHYVKWTQWIFLKFYNSWFDESLQKARPI
                     SELPIPDNLTGPEREKYINDRRLAYEAEAPVNWCPALGTVLANEEVVGGVSERGGYPV
                     IRKPMRQWMLRITKYAERLLEGLKDLDWSESIKKLQQDWIGKSVGAEVDFKIDGSSDI
                     IRIFTTRPDTLFGATYMVMAPEHKLVEKITTAAYKEAVETYKQEAARKSDLDRTDLAK
                     EKTGQFTGAYAVNPVNGQKIPIWISDYVLISYGTGAIMAVPAHDERDFEFATKFNLPI
                     IQVVEPDDPEQAQLCRQGKLCFTGDGTAINSGPFTGLRTPQFKEQITAWLEKEGLGKK
                     AVNYKLRDWLFSRQRYWGEPFPILHTEDGRIVALDENQLPLALPDLEDFKPSDSGEPP
                     LAKAKEWVNVTLSDGTKAKRETNTMPQWAGSCWYYLRYIDPHNPAAAADFEKQKYWLP
                     VDLYIGGAEHAVLHLLYSRFWHKLLYDLGYVSTPEPYQKLINQGMILGEDNQKMSKSR
                     GNVINPDQVVNDYGADSMRLYEMFMGPLEATKPWSMQGVEGVHRFLQRVWRMIIDPQS
                     GQVLDNISDSQADEATVRLLHQTIKKVTEDIDGFAFNTAISQMMIFVNHLSRQEVRPK
                     KIVEQFVLILSPFAPHIAEELWQRLGHTQTLAYEPWPAFDANLAKEKEIEMAVQVLGK
                     IKDKIVVPADAGDDQIEQAALASEKVKTAMAGKLPKKIIVIKPRLVNIVV"
     gene            11714..12268
                     /gene="cyaB"
                     /locus_tag="PLE88_00050"
     CDS             11714..12268
                     /gene="cyaB"
                     /locus_tag="PLE88_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOM74890.1"
                     /translation="MAQEIEAKIKVEALEPTAQRLTELGARFSAVQHQHDVYFHDADG
                     LLKKRGCGLRLREETVNGKSVFILTFKGPRQDSPFKSRPEFQTQVSDSAEMIKILEGL
                     GYQPVLTVEKKRSLWQMDGCDICLDEVEMLGPFVEIEGPNEESIRRILNKLGLQNKLH
                     LSQSYSSMIAHKLQAEMLNRKSQG"
     gene            12269..13216
                     /locus_tag="PLE88_00055"
     CDS             12269..13216
                     /locus_tag="PLE88_00055"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOM74891.1"
                     /translation="MMKPQDGYIQYIVNPKSGASSSKRMVSQFRDYLSEKGFDVRVQF
                     TASLTHAGQLAAEAAADEGCMLVAGAGGDGTLREVVQGLKGTGKLFLPVPCGTENLLA
                     NELGFDQHLQTLINAFEGNCIKPLDLGMINGCCFTSIVGFGFDGSVVDRISKIRTGHI
                     NHLTYFWPIWRTFWEYSFPPVRVSVDGKEIFCNRGLVFVGNISHYAIGLGICKKADYS
                     DGLLDVCIFPCHDQAHLLAYAFKTALKTHLKSRSTIYVRAKTIQVSDCGQPVLCEIDG
                     DPGPSLPADIHILPQAVRVIVPPGAKPAGIRTRFRRIID"
     gene            13353..14177
                     /gene="kdsA"
                     /locus_tag="PLE88_00060"
     CDS             13353..14177
                     /gene="kdsA"
                     /locus_tag="PLE88_00060"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOM74892.1"
                     /translation="MQKIFEIGTVPVGFGQPLFIIAGPCVIESESLCLHVADFLVNLQ
                     RSSGVKCIFKASFDKANRTSIDSFRGPGMEKGLSILERIRNQTGLPVLTDVHEPSQAK
                     PVGEVVDCLQIPAFLCRQTDLLVACGQTGKPVNIKKGQFVSPEEMKNCVEKVRSTGNN
                     KIMLTERGTFFGYNRLVNDMTSIPAMQQFGCPVVFDTTHSTQRPGGLGIATAGKPDMA
                     PVLARAAVAAGANALFMEVHPNPAEALCDAACMLPLDNVQSLVESCRKIYEVVNNK"
     gene            14174..15109
                     /locus_tag="PLE88_00065"
     CDS             14174..15109
                     /locus_tag="PLE88_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM74893.1"
                     /translation="MTQKRYKYLFGPVPSRRLGQSLGVDIIPFKTCTQNCIYCQLGKD
                     AKQTIERRDYVPINAVLEELRQKIEEGLRADYITISGSGEPTLHIHLGQLIDGIRQLT
                     QIPIAVITNGTLFSRSDVRADCCKADVVLPSLDAGDEDTFKKINLPHKQLDFAVFVEG
                     LMQFRREYTGQFWLEVFLCEGINTDETALSNLKRWIDTINPDKIQINTSVRPVTHPQA
                     VRVPLGKLEQIARFFGPHAEVIADYSKHSSQDTIIPDEEYILKTLQRRPCSLDDLCRG
                     LGLAAEAIQSALDNLLASRKISCDEQHGKKFYTAN"
     gene            15347..15598
                     /locus_tag="PLE88_00070"
     CDS             15347..15598
                     /locus_tag="PLE88_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74894.1"
                     /translation="MAQIKKQLVLNKGSEKFIFRYDNGNEGQLLDALVAAATNPNTNF
                     DWFDAAVLSFKLTQSLIHEADDILYKDLSNPVHTYPQGS"
     gene            15661..16671
                     /gene="xerC"
                     /locus_tag="PLE88_00075"
     CDS             15661..16671
                     /gene="xerC"
                     /locus_tag="PLE88_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOM74895.1"
                     /translation="MDNSAIIQEFISYLKFEKHFSEHTAKCYHADLEQFVSFLADAYS
                     GHASPSDHPADNPWDTTSATATAVQTKPATTVDELVKSADVNTIRRYMAELSQHQYCK
                     STTARKLATLRSFYKFMVKRNYLTSNPVVSIKTPKQEKKLPKFLEYEQVIRLLNTPPV
                     NTWLGARDRAMLEVLYSTGMRVSELVALNMDDVDFLGEVIHVRGKGKKERLCPIGSSA
                     LQAIQNYIEFRNRRMSNDSNFDSKVLFANKHGKRLSTRSVRRKMDKYLTEAGLDPSIS
                     PHTLRHSFATHMLNNGADLRSVQELLGHQSLSTTQIYTHITTSRMNEQYHSAHPLEQH
                     QP"
     gene            complement(16735..18873)
                     /locus_tag="PLE88_00080"
     CDS             complement(16735..18873)
                     /locus_tag="PLE88_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOM74896.1"
                     /translation="MPATEIQQHNQQTKKPIFRYRRLLIILAHISCFFAALLVAFTLA
                     FAYGDYSSRRRLVFQLPILFAAVLPVKLFVFARLKQFHGWWRYVGLSDLLMITKASFI
                     SWLVLLVFWYGYVYWGEKIIPLSVIDKLENKLLFYKELSSNSVIAAERTEYGQKYANL
                     KGLYDLVRVARIPEVVRQQKKTAVNSESRQLLEVQYIELNDIKRSIEGVLVLDLFATV
                     IVLSGLRMLVRLYHEEFFSEVKGPVRRFLIVGAGNAGEALLREMLRRKDVNYQVVGLI
                     DDDPAKIGMSIHGITVLGNVDQLPEICRKNAIDEIAIAMPGATRKQMRRVIQVCQGTK
                     LRFSTVPSLTDIASGKLQVSQMRTVDINDLLGREVVNLDLGQIETFLKDKIILVTGAG
                     GSIGSEMCRQVCAFRPRQLLLVEQAENPLFFIDRELRQKFPEVHTEAIICDIVERARV
                     FQIFDMYRPQIVIHAAAHKHVPLMETNPGEAIKNNVVGTCNIADAADAFGADNFVMIS
                     TDKAVNPTSIMGSSKRIAEMYIQDLNATSKTHFVTVRFGNVLGSNGSVIPIFNQQIAA
                     GGPITVTHPEMRRYFMTIPEASQLVLQAAAMGQGGEIFVLDMGEPVKIVDLARELITL
                     SGFRPGEDIEIEFTGLRPGEKLFEELSIEGEDMLPTKHPKIAVWKNIPKDRQSLRAGI
                     EKLIAVAATQDRSKIVAVIKELVPNYIGKD"
     gene            complement(18845..20170)
                     /locus_tag="PLE88_00085"
     CDS             complement(18845..20170)
                     /locus_tag="PLE88_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74897.1"
                     /translation="MTQRYSLKSIWEESALPDLAYSFKLALGPAKIIIAFSGVLAVCV
                     LGAVMDMCSQNVIVYRPETAAAGLASRSELDIYIAANPAQTKRFIKEHQTQTYRQGVF
                     STLWKFAAGRFHAAVFQLLNLDNANLFANVKFALTNIWLCIRAVGWAFRFHPMYSLIF
                     FTAAFLVFVFTGGTICRCAALEFAQGEKPGLLEAVSYVTENCRAFLSAPLLPMGLVSL
                     AAFVIFAVGLAGSISHAGELIIALLFGLLLVFGFVITVMVMGTIAGGLLLFPAIAYEK
                     TTGLDSIGRSFCYVLSCPVRMFYYVLVSGIFGTFFYLIIRLVLFGILRFTYGLLLAGM
                     MTAGAGSKLERIWPKPDFMDFLKRSSSAVGWTESAASIVIYVFMLVIVGLLLSYVVSY
                     FFSTATVIYALMRRKVDNVELNRIYIHLQQIGKNIPLDPRNACNRDSTA"
     gene            complement(20524..21228)
                     /locus_tag="PLE88_00090"
     CDS             complement(20524..21228)
                     /locus_tag="PLE88_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOM74898.1"
                     /translation="MIKRTKKKEHKTAAGKGNRVPSAGKKASKKTGPPEKNTSRQKSK
                     SSPVNPSSEPLLEIQKTWLSEQDLQHFKTLLVEKLNEITGDVYQIESGALKSSRSDAS
                     GDLSSMPIHMADIGSDNYEQEFALGLMDSERKLVAEILAALKRIQNRTYGICEGTGNP
                     IPRQRLEGIPWTRYCLEYAQQMERGKAAEETAASQTAASSKQVNLYQEQESEIQEQTN
                     TGIFDVDSNKSDDENY"
     gene            complement(21381..22178)
                     /locus_tag="PLE88_00095"
     CDS             complement(21381..22178)
                     /locus_tag="PLE88_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM74899.1"
                     /translation="MGFIELQSIWFGYDNTPVLQNLSVSIEANSFWAIVGPNGAGKTT
                     FLRLLSGQLKPRQGAVGLDSRPLESWSPSQRAQKMSYVRQEFVPVFDFTVFETVLMAR
                     SFLRKWSLFETAHDREAAESALKQTDTYQLADRNLAQISAGERQRVFIARALAQQTPI
                     LLLDEPTSHLDMKHQIQIFDLLRRMQVEQGKTILMVTHDLNLACQYSDYVLLVGKDGC
                     YATGKPNELLTAEIIEQFFSVKVHQGLVCAEKFFLPLGNYSKDKRKY"
     gene            complement(22187..23203)
                     /locus_tag="PLE88_00100"
     CDS             complement(22187..23203)
                     /locus_tag="PLE88_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOM74900.1"
                     /translation="MKILTCGKLTLRILAAGVLLIAVMIACTSIGSESVSLRKAMQGE
                     KNASNIDYEIIMHFRLPRVLLASVVGASLACAGVVFQAILRNPLADPYILGISSGAGL
                     GSMLAVILGWTWTFGGLSAMMAASFIGAMATVWLVWGIGRMTGRLQTTGLLLAGVVVN
                     AFLSAGILFLISIVKAGHLQTTLFWLMGSFAELEYGPVLGAAGGICGFGIIVLMLLGQ
                     RLNILSFGPDEARSMGVPVEQTYFLAFAAAALMTSAAVSLSGLIGFVGLIVPHAVRLI
                     AGPDHRQLIPLSALTGAAFLAAADAAARIAVAPAILPVGVITALAGGPFFLILLVRCT
                     RSMH"
     gene            complement(23203..24111)
                     /locus_tag="PLE88_00105"
     CDS             complement(23203..24111)
                     /locus_tag="PLE88_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOM74901.1"
                     /translation="MEVLHLRNQFLRAVCWSIAVVLWTAVWAGCSKKEIPAGGRPDSS
                     LRIIAMAPNLTEILFALGLDKEIVAVAKGSNYPPAALQKRTVGTFWQPDIEAVLACRP
                     TLVITESFAQQAALAERLRRMGCRTLTLEIETIEQLHQAILTIGDAVHRKEAAEQIST
                     QLKDKQRGIAARCEALPYRPKVLWVIQRQPLRAAGIRAFPNELIETAGGINAIGDTPH
                     QYPPIAAETVLAAKPDVIIETADDAQSLKRQQETASQFYRRFAGVPAVQTGRIYVLDG
                     DLVSRLGPRLAEGMEQVAECLHPREN"
     gene            complement(24119..25960)
                     /gene="mutL"
                     /locus_tag="PLE88_00110"
     CDS             complement(24119..25960)
                     /gene="mutL"
                     /locus_tag="PLE88_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOM74902.1"
                     /translation="MGKIAVLDDRMINMIAAGEVIERPASVVKELLENSIDAGASQIV
                     VEVEGGGRDLIRVTDDGAGMDAADLALAFEPHATSKVRCQEDLSAIRTMGFRGEALAS
                     IGAVAKVSAVSRTADSIEAAVVEIDCGQKKPVRPCSGAVGTVIEVRSLFYKIPARRKF
                     LRTPNTEMTHIIEHFTRIALAHSSLDLTLIHNGRQVYHLLAGQSIRERVAILFGQAMA
                     ADLLETSRAERGIQIQALLAKPAAARASANYQYLFLNGRFIRDKFLLHAVREAYRGLL
                     EPNKYPVVFIFLQMPPEDYDVNVHPTKVEVRFDNPNLVHSEVLAVLRDKLLSTNLDAV
                     GTIPCASDEASRLSAMPLTSGTVARRERIQRAMEDFFKQQPSYSQAKFSFLPDSLSGK
                     NFSASLTDGPQTLQTEAPAAESSEQAERWHAREYLQVHNSYILLQSEDGFAVIDQHAL
                     HERILYEQMCKRLREGSLPSQRLLVPHSFEVTRAQLEAYESQKELFEKLGIELVPFGP
                     NTLAVQAFTALLENADPADFVRETLEKLAGESGHGDPERLLHEILDMAACKAAIKAGQ
                     PLSEQEIASLLKDRDIILRASRCPHGRPTTITFSLRELEKQFKRTGF"
     gene            complement(26015..27583)
                     /gene="gpmI"
                     /locus_tag="PLE88_00115"
     CDS             complement(26015..27583)
                     /gene="gpmI"
                     /locus_tag="PLE88_00115"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOM74903.1"
                     /translation="MSEHKITLRKRPCVMIIRDGWGYNPRPEEDAFNAVKVAKPPVDA
                     ELMAQYPHCLVRTYGEDVGLPEGTMGNSEVGHQNIGAGRVVYQDSVRITVAIREGEFF
                     TNPILNAAIDNALQKKSKLHLMGLCSDIGVHSLLGHLYGLLELARRRGIQRVYLHAFT
                     DGRDSPPTSGRDYLKAIESKMAEIGVGQIASVMGRFYAMDRDKRWERVAQAYKCLTQG
                     IGNKARSAAEAVEASYGREETDEFIIPTAIVDGGGKPLALVEDGDSVIFFNFRGDRPR
                     EITRAFVDNPFDAFERTVHPEVHYVCMTEYDASIKAPVAFPPIRELPNIAGEYFSRLG
                     LKQFRCAETEKYAHVTFFFNGGREAPFEGEDRQIIPSPKVRTYDLKPEMSANEVCLEV
                     LSRLDSGKYDAVICNFANPDMVGHTGVLDAAVTACRTVDACVGRIVDKTLALGGSAVI
                     LADHGNFERMWDFENNMPHTAHTVGDVPLIVVDKEFKGRAMRPGRLADVVPTFLEVMG
                     LPKPAEMTGRSLLI"
     gene            27877..28926
                     /locus_tag="PLE88_00120"
     CDS             27877..28926
                     /locus_tag="PLE88_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM74904.1"
                     /translation="MLSKKCILMQFMAAVSFVLVFASGCQSPQTAVSDPNAVISAAQN
                     QPAPQASIDPKEQAVSLYVDAMMLNDLNEREEAIRKINQALELDPHFSMAYSLKGDIL
                     QGMEQYEQSADAYEQATLYDPWSFKDFFNLGKVCQIIKQWARAAKAYVSACTLDPQHY
                     SAHLGAAQCFYELKEYENSLIYAQKAKELDPAQAAPERLLGDLYASQKDHLQAINAYR
                     RVLEMEGNKPDVMISLARAYLRSGRYNSAKEVLTDVIAIEPQNSLAYQYLGFAQLKLK
                     ETEQAVESYQKAVNVGQDDWMAHKGLGVAYMLLSMKKQNDEKIQALAIEQWTISLQIK
                     PDQPKLQQLIERYSR"
     gene            28945..29520
                     /gene="lspA"
                     /locus_tag="PLE88_00125"
     CDS             28945..29520
                     /gene="lspA"
                     /locus_tag="PLE88_00125"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOM74905.1"
                     /translation="MKDKKTHYAQPDNTVLLHTAAVSMTQKILLFWPAAAGGAALDLW
                     SKWAVFRWLSTEEGYRYSIIDGFLQFVLRENEGAAFSLFRGWTAFLVIISAGALVFVI
                     GLFFFAKFTSKLVLFSMGCITAGIIGNLYDRIFNDGRVRDFIDVYVGPYHWPTFNAAD
                     SLLCIGVGLLIIANFTSPAAQRHDLPQTEAH"
     gene            complement(29468..29866)
                     /locus_tag="PLE88_00130"
     CDS             complement(29468..29866)
                     /locus_tag="PLE88_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOM74906.1"
                     /translation="MEIPVELAQIIINEEADQQIIVLRELNGQQRAFPIVIGLPEILA
                     IDRRLKGIELPRPMTHDLLANVIEQMGGTILKVVINDLREHTFFAVIYIRQGNSVVAV
                     DSRPSDAIALHAGLNVPLFVEDHVFEQLAM"
     gene            complement(30325..32511)
                     /locus_tag="PLE88_00135"
     CDS             complement(30325..32511)
                     /locus_tag="PLE88_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM74907.1"
                     /translation="MRNCRKVWMTGVVVCLFAAFANAADLVSYTASGSPGSSPDANNG
                     TVDVWAVTGDGGASRSFLKDMQDGNPYMWTIWDLDGGAGTYATHTFAGGPLTVGQSVS
                     IDWAHNISIDNGTSIGIRLLNGSSPEVALVFLGGKIVYTRWDTGTGAYTDIAKYYDRY
                     DIFQVVFTLTGPNSYEMTVSEGSIPDNPAGWGNNADDGNSNVGPIIDRWVGTFTGTAI
                     TGIQVYTEGGNDSDQWFDNLRIHEDWLKTAHAPMPSYKQEYVPVAGLELSWAIPQIRS
                     SVNPQVMVADPNLVSFKLYYSCTDPNLNAASPIDVTVWDPQTLRAAYVPAQELQKNST
                     YRWRVDSVMNDNTARQGIEWIFYTEFSKPIILSHPGHQIIDAGGTAVFSVTVDSQSPT
                     VYQWYRVAGGEDILLTDGGNISGTGTNTLAIADAGLDDEGYYFCVVNNESGIPAESIK
                     APLGIKRRIAYWPFENNVFGSSVAGSPHTIVVGDPNFVPAGILGNAVEFTDGVDMLFM
                     DPEQNAYFDICNYEMTVACWVKTTDRQSWSPLVARNGEDGQGWQLRQSGFTNDRPCFT
                     TRTPNDTDNDDGTPANRTIYDGIWHYVVGTYDGAVKKVYIDGVVSRLYSSDSGDVVRE
                     SDPVSGPIGSTGSPVSIAGRVRGNLTEGLNVELGSVVAGIYDEIEIYNYALDAETIAQ
                     KYADLAGTNVCLSQTYDLNNDCVVNLADLGLLASEWLNSRLFVPMP"
     gene            32970..34529
                     /locus_tag="PLE88_00140"
     CDS             32970..34529
                     /locus_tag="PLE88_00140"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOM74908.1"
                     /translation="MKMYKVLLVCVLLIPLQGFSKTTIQDADGVNRLTIYADQEKGTI
                     APEIYGHFSEHLGRCIYDGIWVGPNSNIPNIRGIRKDVLEALKNLNIPVLRWPGGCFA
                     DEYHWKDGIGPRQQRPRTVNTHWGMVTEDNSFGTHEFLDLCELLGCKAYIAGNVGSGT
                     PQEMMEWVEYMTFDGDSTMANLRRQNGRDKPWKIDYFGVGNENWGCGGNMTPEYYADV
                     YKRYQTFVKNYPGSRITKIACGPNGNDTRWMEVVLGRAGQQIQAISVHYYVMSGSWGN
                     KGSATQFDENEWFSLMDKTVGVRKLLQDQISVMDRFDPRKRIALYVDEWGTWWDQEPG
                     SQPGFLYQQNTLRDAVSAGIYLNEFNNHCDRVKMANIAQTVNVLQAMILTEGPKMLLT
                     PTYHVFEMYKVHQGARLLENELVCNAYAKGGTKLAALNVSASKKDGVISVSICNLDPT
                     APAELECRLDGMQAQKASGRVLTAEAMNAHNTFDKPNAIQPTELKGIEIKDGLVRATL
                     PAKSVAILIIQ"
     gene            complement(34594..35370)
                     /gene="larB"
                     /locus_tag="PLE88_00145"
     CDS             complement(34594..35370)
                     /gene="larB"
                     /locus_tag="PLE88_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOM74909.1"
                     /translation="MNRKQIETLLTDVREGRMAVCEAVERLRHLPFEDIGFAKVDHHR
                     SLRCGFPEVIYCPGKTVEQIAEIFQRLAPTGHTILATRASAEVFERVKSTANLDALRY
                     EQAARIIILQQRPIEASVGRIVIVTAGTADMPAAEEARVTAQMLGQNVQMVCDVGVAG
                     LHRLLGHLEVLRQANVVIVVAGMEGALASVVGGLVDCPVIAVPTSVGYGASFEGLAAL
                     LAMLNSCAAGVTVVNIDNGFGAAYAASLINMKIEQSRPAD"
     gene            complement(35387..37348)
                     /locus_tag="PLE88_00150"
     CDS             complement(35387..37348)
                     /locus_tag="PLE88_00150"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOM74910.1"
                     /translation="MKLNQIFHAAVLMSTAAIASAQSTITISADQPGTPIASTMWGIF
                     FEDINFGADGGLYAELVKNRSFEFDNPMMGWSLILSADSDGQTAVVVKEPVDEKNPHY
                     LKMSASRGQPFGIANEGFRGMGIRQGRSYWFSVFARNIGSPAVSLRIEVVSGTEKLME
                     GRIENITGDWKNYTCKMEAMATEPKAHLEIFMDSFGTVDLDMISLFPADSAQMADRPI
                     PGLREDIVAMLADLKPGFLRFPGGCIVEGVTLSNRYQWKNTIGDINQRKTIINRWNTE
                     FKHRLTPDYYQSFGLGFFEYFLLCEKIGAEPMPIINCGMACQFNTGETAAVDKLGPYV
                     QDALDLIEFANGPADSPWGRKRADMGHPKPFNMKFLGIGNEQWGPQYIERYKVFVKAI
                     KEKYPDIKLIGATGSDPAIFPNGPKEVEYLWQELRRLNADIVDEHFYRPPDWFIQNAA
                     RYDAYKRSGPRIFIGEYAAISQNVGSPNNRNNLAGALAEAAFMTGLERNADLVRMAAY
                     APLTAHVDAWQWKPNLIWFDNLSVYGTPSYYVQKLFSNYRGDALLPAECRQTQTLFVS
                     AVRSGKSIILKAVNPSSKSVAADINLKGITSVSSAQSVILTGLPQDENTIDQPAKVLP
                     VNEPVNVQGPAFEYLFKPYSMTVLQLTVQ"
     gene            complement(37380..38426)
                     /gene="tsaD"
                     /locus_tag="PLE88_00155"
     CDS             complement(37380..38426)
                     /gene="tsaD"
                     /locus_tag="PLE88_00155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOM74911.1"
                     /translation="MKGTDLAEQGIHILGIETSCDETAAAVVANGRQIKSSVVASQTA
                     LHEQYGGVVPEIASRAHVENILPVIEKAIQQACIEPSQIDAVAVASQPGLAIALMVGV
                     TAAKTLAFAWGKPLIAVNHVHAHLQSAILEQESIELPAVALIVSGGHTNLYDAESALD
                     LRLLGRTIDDAAGEAFDKVASILGLPYPGGPSIEKIAKEGNPKAIRFPRSLMQKDNLN
                     FSFSGLKTAVLYHCRGQDMKGQNRAASMSRQETADIAASFQAAVVEVLVRKTRRAARQ
                     IGAKTVLLGGGVACNSALRQAMEAMCSREHLTLLIAPKQLCTDNAVMVASLAYYKYQA
                     KQFAGLDLEPSATA"
     gene            38553..39740
                     /locus_tag="PLE88_00160"
     CDS             38553..39740
                     /locus_tag="PLE88_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74912.1"
                     /translation="MTTRESNKNVFFELIQTEEGLAKLIGLIKCVHTVALDTEADSFH
                     HYRPKVCLIQLSFNGYTAIVDPLAGVSLKPFLEELAVKELVIHDAGYDLRMLKGDFNF
                     VPQNGIFDTMLAAALTGMANVGLSSVLQEVLQIQIAKHNQKADWSRRPLPDYLLRYAA
                     EDTCYLMQIRDNLESRLNELGRLDWHRESCRQAVGSAIAAKETPDIDNLWRIKGSGIL
                     TDRQMAFLREIWHWRHSIAKRTNVAPFMICRNEDVVKLAIWASHHRKPVEPDAQLPIR
                     CKASYRNALLTALQKAHCLPKDQWPSLPPSDPSKRLPDSVRKIVNILKTECETIAKKN
                     GLPLQWIASRAALTEIVLKQATSLEQIQKEQILLNWQAKLILPAISKVLAEGGQTDHR
                     LSE"
     gene            complement(39722..40687)
                     /locus_tag="PLE88_00165"
     CDS             complement(39722..40687)
                     /locus_tag="PLE88_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOM74913.1"
                     /translation="MNQTSLVALQRCQSYDAQQVYNAVCRQFELLGGVDAFFGRNQRV
                     LIKPNLIVPYSPEIPAQTHPEVIYAAARCVKDAGGKPLVGDSPAWGNVWACLKALGVD
                     KRLKHLGVEIVQLNNPVPIQIEDSVVRISRTALEADAIINLPKLKTHQQLTATFAFKN
                     MFGCVAGLGGKEKAWWHFARGDRVESFCRLIIGIFQRLNPVLNLIDGIVGMEGQGPIN
                     GSPRQIGYLIASRNPAACERICCQLVGFNPKDLPLLQTAEQMGYTLDSTLEITGDTFD
                     GPICPDFVPAVQTPLRFSLPRICKSITLQGWMLLKNKWPRHSDKR"
     gene            complement(40689..41468)
                     /locus_tag="PLE88_00170"
     CDS             complement(40689..41468)
                     /locus_tag="PLE88_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74914.1"
                     /translation="MTGVGLLWGCAVSPQSMPAPVPVEGLKLSDLKDSANFKPNAEYL
                     IELRVLTYVLDSASADELKTVYDMLSQQAIRTANRDVFALNGLAIASGPSQEASRIAQ
                     RLYEIGAVRISQSKLLIPPEEIQILSSTFQQQGLTFLYCPFQGRINEIVLPPSTFIGW
                     TMLARPDPRLEKHVQLTFVPSYWQRGAEDLRLRMGKQPIDFKPLEAGRVLARLEEGGF
                     LLLGPNGKMPEQNTLGKILFLLAGRKPQMQFFVILCDKAGL"
     gene            complement(41551..41949)
                     /locus_tag="PLE88_00175"
     CDS             complement(41551..41949)
                     /locus_tag="PLE88_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM74915.1"
                     /translation="MYFAHYYENAFLYPYFCKEAEKMEEERRRHKRFPLKLSIFCQKV
                     GISDGRIYSGRTINVSPGGMLVEMKAGDISDGQLLSVEMTVPPTEGLLDFGGSFSSYA
                     RVVRSDVGTSQESTEKTLALEFCDLPKLRV"
     gene            42309..42953
                     /locus_tag="PLE88_00180"
     CDS             42309..42953
                     /locus_tag="PLE88_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOM74916.1"
                     /translation="MHNHSLLQFDALCKMIAEPLHLGLISFDPLLNIIDYTPSVAKFV
                     YLEPTVDLALLRGTDTNIWKDWKSLLLSSLHTGQKADFGTVKYMHEGQVRLLNLSCLP
                     VRNSDAADLLGGVLAFHDTTEKSDIEHGLAQAERLIAIGKVTGKVAHELNNPLDGILR
                     YVNLSMRIIEQGQPQKAMEYLQHCRTGLQRMTQVITELLEFSRSTHLAFETSPV"
     gene            43006..43386
                     /locus_tag="PLE88_00185"
     CDS             43006..43386
                     /locus_tag="PLE88_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM74917.1"
                     /translation="MKIQREYTAPLPHLKSDSLFQVFCNLIKNAADAMEGRGQLTITI
                     RQTSSEWQIEFRDTGPGIDPKHMDDIFKPFFTTKPQGKGTGLGLAICKDILAKLDGRI
                     TAQNAPQGGGLFTVYLPLHNPNRS"
     gene            43389..44768
                     /locus_tag="PLE88_00190"
     CDS             43389..44768
                     /locus_tag="PLE88_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM74918.1"
                     /translation="MPKASILIADDDAIITNSLCEFLTMEGYQTAGVGTVAQARQKLK
                     EQPFALVITDVNLPDGDGFDILDIVRANYPQTVVLLITGYGTIESAVEAIKKGAYDYL
                     TKPIVDDDLLLAVERAIRQQSLMSENQQLRQQIENKYRLNNIISQNYKMSRIFELIEA
                     VADSKTTILMTGASGTGKSLLAKAIHFRSLRRDQPFVEVSCGALPETLLESELFGHVK
                     GAFTGAISDKEGKFLAANGGTIFLDEISSASPAMQVKLLRVLQERQFEPVGSNRTITV
                     DTRVILASNRDLAEEVKQGRFREDLYYRINVVAINLPPLAERIGDVPLLADHFLKHFC
                     LVHNRRKSGLTDQALHCLQSYHWPGNVRELENVIERAVLLSKGPLIDVEDLPQNVVQN
                     SHRLGNYTHQSLKEALEGPERAIIRAALEANNWNRQETAKALDINRTTLYKKMKRYGL
                     EEEAQRIGL"
     gene            complement(44827..45207)
                     /gene="rnpB"
                     /locus_tag="PLE88_00195"
     ncRNA           complement(44827..45207)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLE88_00195"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            45388..47304
                     /locus_tag="PLE88_00200"
     CDS             45388..47304
                     /locus_tag="PLE88_00200"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOM74919.1"
                     /translation="MSQKKILLVQIVCLFLVLDISCVCLSAEINPYGICAHVSRPSDH
                     NLAEQEFELMRQAGIYWVRTDFDWTTVQQQQDGPWDYSVLDATVNKAEQAGIQILPIL
                     AYDTPWARPAYKHLDKWIEYVRNTVSRYHRQLRYWEIWNEPDLEQFWKDKPNPADYTS
                     LLKAAYQEIKKIDPELKVLLGGLSGIPYEYIEGIYRAGGKDFFDIMAVHPYRYPDAPE
                     LNSLRNDLDKLRQLMSRFGDADKPIWITEIGWPTHQNGVPLLDDIIRCALNYLEPSRK
                     NWTLAVLSIPEYPPRYSYSDERLQGMLPGGGRIVHLNIETINFLNPQIHNALLMPPDE
                     AFPADCFDIIEDYVRKGGIILFTQGVPLYYTIRQDSQGNWTRNNADSSYRKRLHIGWE
                     AWWTRPGVPRQISTLLVPIDFRTQVHLPGTQITAERFLTDSELKTGDKFIPLLEAAEG
                     DYKGSAAAVFDLDSDLKGAVIVSALIQDLRGISEEQQAAILPRALLAALHSGAECVFW
                     YNFRAFENNPYYNEDHFGIIHKDLRPKPAYWGIQSLTRARPAGSKPLMENWQTEDLFY
                     PGWKRPDGQNGYAVWTTGKPRYAGFLAEGKISGMFDNSGSPLAVEQSNNMIRLFVTET
                     PLYLIGPEKLLPAQ"
     gene            47429..50629
                     /gene="carB"
                     /locus_tag="PLE88_00205"
     CDS             47429..50629
                     /gene="carB"
                     /locus_tag="PLE88_00205"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOM74920.1"
                     /translation="MPKRKDIHKVMIIGSGPIIIGQACEFDYSGTQACKALRKLGYQI
                     VLVNSNPATIMTDPGMADVTYIEPLNLESMTKIIQKERPDALLPNLGGQSGLNLASEL
                     AKAGVLDKYGVKVIGVQVDAIERGEDRIEFKKTMDKLGIDMPKSQPAYSVEEAEAIAG
                     QMGFPLVIRPAYTMGGTGGGLVYNMDELKTIAARGIAASLVGQILVEESVLGWEELEL
                     EVVRDAKGQKITVCFIENVDAMGIHTGDSYCTAPMLTISQELQNRLQKYSYDIVDAIG
                     VIGGTNVQFAHDPQTDRVVVIEINPRTSRSSALASKATGFPIAMVSSMLAGGLTLDEI
                     PYWRSGTLEKYTPSGDYVVVKFARWAFEKFKGAEDKLGTQMRAVGEVMSIGKNYKEAF
                     QKAIRSLEIGRYGLGFAKNFNSLSLEELLQRLNFPTSERQFLMYEALRKGADIDILHK
                     KTHIKKWFIQQMKELVDLEETILKYKGKPLPDALLKQAKQDGFADRYLAQLLSVSEES
                     IRKQRTKLGIVEGWEAVPVSGVENAAYYFSTYNAPDQVGSSNRRKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFTLREEGFETIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYEK
                     EKPEGIIVQFGGQTPLNIARQLEEAGVKIIGTTTETIDLAEDRDRFRIMMEKLGIPMP
                     QSGMASTLQGALEIAEKIGYPLMVRPSYVLGGRGMEVIYDEDMLRRYVAAAVEVTPER
                     PILIDKFLVNAIEAEADAIADGTDAFVPAVMEHIELAGIHSGDSACVIPPVSIPQKHL
                     DTIYEYTRKIAINLNVVGLMNIQYAIADDVVYVLEANPRASRTVPLVSKVCGFPMAKI
                     ATQVMLGKKLSELNVKPKRFLHFGVKEAVFPFRMFPEVDPLLGPEMRSTGEVLGLADS
                     FGLAFFKAQEAAQETLPLSGTVLITVAEQDKAYIAETARRFAEMGFKILATDGTHRFL
                     TGKGIASERILKLYEGRPNIIDRIKNKEIQLIINTPVGKQSQTDDSYIRKAAIRYKIP
                     YITTATAAAATAKGIAARLKSKSDVKSLQDYHKEIL"
     gene            50809..52392
                     /gene="cimA"
                     /locus_tag="PLE88_00210"
     CDS             50809..52392
                     /gene="cimA"
                     /locus_tag="PLE88_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOM74921.1"
                     /translation="MRKIKIYDTTLRDGMQAEGISFSVEDKLLIARKLDDFGIDYIEG
                     GYPQSNPKEEEFFACVRQIPFRNAKLTAFGSTRRAQTKIEDDICIRALLASGAPAVTM
                     VGKTWDLHVTDVLRCSLDENIAMCAESIAYVKKQGREVLFDAEHFFDGYKNNPDYAMK
                     VLQAAAEAGADALVLCETNGGCLPHEVFDITQTVCRTFTGIEVGIHCHNDSDCAAANS
                     LAAVRAGAVHVQGTINGLGERTGNANLCTIIPNLSLKMGYETVGSEQLKTLTEVSRFV
                     FEIANLPLVTQMPYVGESAFAHKAGLHIDALRKNKKTYEHISPEAVGNERRFLISELS
                     GSSNVLDRLEKRKIITDKAAARKILNRVQELENQGYQFETAEASFELLVKKELGTYRR
                     SFDLLKYHIDVERSPDGKMITEATVKLQVDGKTEHVVSEGDGPVNALDGALRKALEPF
                     YPQLRDMTLIDYKVRVVNARAGTAARVRVIIESRDKDGSVFGTVGVSENIIEASWLAL
                     VDSVEYKLLKAAEKLEQTV"
     gene            52616..53530
                     /locus_tag="PLE88_00215"
     CDS             52616..53530
                     /locus_tag="PLE88_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOM74922.1"
                     /translation="MNRRSFIAVGTAATVLGSTALTTDTHAADTSASSQRFKLKYAPG
                     PGMFKNLAGDNVLDQIKFIHDQGFGAFFDNGLMGRPADMQERIAQTLSGLGMELGPFV
                     LYADFGVKSFVLSSADTRKMLTDKARQGVETAKRTGAKWALMVPGRYDESLEWDYQTA
                     NVIDNLKYCADICGQGGLTIVIEPLNPHDHPGLFLTKIPQAYQICKAVGSPFCKIVND
                     LYHQQITEGNLIPNINRAWSEIAAFHIGDNPGRKEPGTGEINYKNIFRHIHQKGYTGV
                     LCMEHGLSSGGREGEQALLAAYRECDSF"
     gene            53543..54403
                     /locus_tag="PLE88_00220"
     CDS             53543..54403
                     /locus_tag="PLE88_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOM74923.1"
                     /translation="MTADITRRGFLAGSTAGLAAGLAGSAWADEPALKLKGRIRQSVC
                     RWCYSSIPLDSFAASCARIGLQSIELVEPADWPILKKHGLICAMTPSHSITKGLNRIE
                     NHPECLAKIRAAIEAASAENFPNVICFSGNRAGLDDAEGLKNCEIAIKQIAGFAEEKK
                     VTLCMELLNSKRNHKDYMCDRTGWGAELVKRVGSPRFKLLYDIYHMQVQEGDVIATIR
                     EYADCIGHYHTAGVPGRNEIDDTQELNYPAIMKAILDSGFGGYVGQEFIPRQKDPLAS
                     LAKAAELCDI"
     gene            54420..55037
                     /locus_tag="PLE88_00225"
     CDS             54420..55037
                     /locus_tag="PLE88_00225"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOM74924.1"
                     /translation="MKKKIFSIVGLILLALSGCASKEELFNGRNLDGWKPYLADASVD
                     PSTVWSVKDGVLRCEGVPNGYLSTVRTYSNYSLHLEWRWVETPTNSGVLLHMNGPDKL
                     WPQCIEAQLKHQDAGDIYLIGGTDCKGITDRQGSRLPKINPTSENTAGQWNRYDIVCR
                     GDTIELHVNGILQNKATQASVTSGKICLQSEGSPIEFRNIKLRPL"
     gene            55083..56354
                     /locus_tag="PLE88_00230"
     CDS             55083..56354
                     /locus_tag="PLE88_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM74925.1"
                     /translation="MKAGLNRREFVKSAAAVAAGTLVSPTGLFAAGSETIKVGLVGCG
                     GRGNGALNDFLNAAKHLNLKVEIAALADFFKERADGTAEKFGVSKDIAYGGADAYKKV
                     MASDAQIVLMATPPISRPAHFEAAVKAGKHIFMEKPVAVDPPGARKIIAMGKEAAQKG
                     LSIVAGTQRRHEAAYLKTKYAIDQGAIGPIRGGAIWWCGGALWYKKREPGESDASYMV
                     RNWVSFTEMSGDHIVEQHVHNIDVANWFIGRTPELAIGFGGRARRKTGNQFDFFSVDF
                     DYGDGVHIHSMCRQINGTYSRVSEYFTGTLGGTWGSGPGKEGFPKKVDIPEIPYVGNP
                     YVQEHVDLLESILNGKPINEAENVATSTLTGIMGRISAYTGQLVRWKDLVDETTGSPW
                     YNLTLSPTEADFENNTVQAPADEICAIPGKE"
     gene            complement(56651..62950)
                     /locus_tag="PLE88_00235"
     CDS             complement(56651..62950)
                     /locus_tag="PLE88_00235"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOM74926.1"
                     /translation="MLSIAAYGGMIHRYSFSGNANDSIGNAHGVLVNNGADAYFTSTT
                     LEMGNSELPNSSSSTINYVDLPNGIISSISPAVTLEAWVTWYGPSNSAWQRIFDIGIS
                     DAGENQSVNSANSGYFFLTPYSGGGTYRFGHRNGIELGTPVELTIDSAALPVNQQVHV
                     AIVWDDVAGQVRMYYNGQRVASGPLLFTLSQLKDVNNWLGRSQWPDPAFNGSYNEFRI
                     YDIALSDAMLLASYQAGPDAPIAAYAAPSNPQPYSGGISGGLTPVLTWQSDTSADITG
                     HRVYLGTDYQSVLKATPESAGIFQGVRPAGCENYTPAAALMMDQTYFWRIEEVTVSGY
                     VFSGPVWNFRTVNLKAHTPFPSNGVQGVGTEGVTLHWQSGAGAVGHQILWGTSPAALS
                     TLEINYPLTSYSLPKLDFETVYYWRIDEIYPSETIIGDVWTFTTMEKPASCLGGDLDG
                     DCQVNLQDAALFSSYWMSNIDCSAFDCPDWDQNRWVDTADLTVLADNWLDSRYPQVVI
                     NEIHYHPDSNKEPVEFVELYNASAMSVDLNGWRLDGAVKYTFRNVPVMHPGAYIVVSE
                     NPEAFQIKFGLPAYGPFEGKLSNEGERLVLRNPTGLIIDEVNYDSEFPWPIAAGGEGA
                     SMELIHPSLDNDLAGSWRSSGYNEAGRPELSYGSPTPGRRNSVYALNAPPQIRQVRHT
                     PQQPRSTEPILITAKISDPDGVKRVYLNYQLVSPGSYIPAYLPIPISSLIADPYQPQP
                     LNPEFENSWITVLMMDNGLFGDAVAGDNIYTCRLSPQSNRVLLRYRIEAWDQNDNSIR
                     VPYADDPSLNFACFIYDGVPDYTTTHASVHPDGVGHSYSSDILTSIPVYTLITRAADL
                     YQCNGYNAADRIDQGTTDVKTQDAGRVYNWEGAFVYEGKVYDHIGYRLRGGNGRYNYG
                     LGGKRSMKFRFNRGNYFQAKDLYGNPFPSKWQHLNTGKMFGNKITEYRKYPYGLNEVM
                     DMLLFNIAGVPAPETYWIHFRVVDGLEEAPAGTQGQYEGDFWGLYLAFENYDGAFLER
                     LNLPKANLYKLSDKIYDGARQLRYQGENAVADSSDYENIRWNLNYQASADFIRRYLDC
                     DEWYRYHTVTEAIRHYDIFSGSTCIHCLKNCAWYFYPNYSAENPYGQLQFLPFDVDDT
                     WGPYFNYGCDHAKGAIYDQMYDGDHVLRQYTAKPEKAPIKQEYRNYIREFRDLLWQPE
                     VINPLIAELASVIADFVPADRDRWRLVSNSIDNGPLEEGVALMEQFAWSAGTFDGKYY
                     WSGTASNLDTLAGAEGDSTNIPNTPTITYIGTPGYPENDLRFRTSAFSGPQGNGTFAA
                     MKWRIAEYENLGGPSTPTQINLINRGALWRYFKGTQEPSAQTGAWRQIGFDDSQWSEG
                     TAPVGYDTNLVMGTSLNDMRGNYSTIYLRKAFTVADLEKIQSLQFAILRDDGFKLWIN
                     GQLALEENVPAQENLPYNTVLESYGLSYIPDETIYKSFEITNPQQYLTAGTNVIAIQV
                     INVSLSGSSDCYADLEVIAKLSAPSEELKKGLSRHKYELQPVWESGELTTFNSDIQIP
                     ADGVKPGRTYRVRCKMKDTTGRWSHWSAPIEFVAGEPIGSEIRDYLRITEVMYNPAAA
                     NPALNELNVDNNEFEFIELKNCSVDKILDLSAVSFTSGVTFSFAGSAVTTLNPGQFVL
                     VVKNAAAFDSRYPGLSSRIAGTYTGSLSNSGEVIKLEDTWNGTLAEFEYNDGRGWPIA
                     ADGTGHSLVPLPNAIVVGRPSSTLNYGGNWRQSAYIGGSPAADDPSLPAGLLINEFMA
                     HTDFSDQNYPGYDSNDWIELYNAGNSMSLDGNWYLSDDGNNLKKWPLPVRSVLSGQFI
                     SFDEVTGFHSPLSTGFGLDKAGEQIFLSYLPGVPNADRIVDCIKFKGQENGFSYGRYP
                     DAGPYWLAMQPSRNAPNIQPLPSIVISEIMYHPQNGTNQDEYIELYNPTDTAIELQTS
                     AGPWALDGAVNFQFSPSIQLPAQTKIVIVGFDPAADAQRLNAFKAVYNNTDLVAGVNI
                     FGPWTGDLSNAGERLTLEKPQDSDDPLVPTAISWIIVDECIYGDAWPWPAEPDGTGMA
                     LGRITITGSGNNPASWGEIEPSPGQ"
     gene            complement(63264..64280)
                     /locus_tag="PLE88_00240"
     CDS             complement(63264..64280)
                     /locus_tag="PLE88_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOM74927.1"
                     /translation="MSIQITNISKTFGTFKALQNVSLTVSHGELVALLGPSGCGKTTL
                     LRIIAGLESPDPGSGPVLFYEDDVSSQSVRQRRVGFVFQHYSLFRHMTIFENVAFGLN
                     VRPRRYRLSKSQIRERVFSLLKLVQLERHADLYPSQLSGGQRQRVALVRSLAVEPKIL
                     LLDEPFGALDTKVRKELRHWLRRLHDEMSITSVFVTHDQEEALELADRIVVMNAGRIE
                     QIGKPEEVYDNPANPFVFSFLGDVNILRSPFGSQSTQPSATASYIRPHDIEISKTALD
                     TGSIQARILHVAAVGPTVKVELARLDTNESIQAEITRQIYKRLNITVGQMVFISLHNC
                     RIFQ"
     gene            complement(64277..65116)
                     /gene="cysW"
                     /locus_tag="PLE88_00245"
     CDS             complement(64277..65116)
                     /gene="cysW"
                     /locus_tag="PLE88_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOM74928.1"
                     /translation="MKNKLPWIPIVLTAAALAFLGLFLLLPLAAVFIEAFRKGIGPYF
                     QTFADPYARSAVRLTLLVVLTAVPFNLIFGLAAAWAIAKFDFRGKGLLLTLIDLPFAV
                     SPVVSGLLFVLLFGARGWLGPWLLEHDIKIIFAAPGIIMATMFVTFPFIARELIPLMQ
                     SQGTEEEQAAMVLGAGGWQIFRRITLPNIKWGLLYGIILCNARAIGEFGAVSVVSGHI
                     RGQTTTIPLHIEILYNEYNFVGAFAMASLLTLLALLTLAVKSLVEWKTRNEQAAAVIG
                     GIE"
     gene            complement(65113..65949)
                     /gene="cysT"
                     /locus_tag="PLE88_00250"
     CDS             complement(65113..65949)
                     /gene="cysT"
                     /locus_tag="PLE88_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOM74929.1"
                     /translation="MQYKPKNVLPGFGLTLGYSALYLCLIVLIPLSALVISAVRMEPH
                     QLWAAMTSPRVLASYRLSFTTSFAAAAVNAVFGLLTAWVLVRYRFPGRKLFDAMVDLP
                     FALPTAVAGITLTTLYAPTGWIGRLLEPLGIQAAFTPLGIVIAMTFVGLPFVVRTVQP
                     VLEDIGKELEEAAASLGATPFQTFRRVIFPELLPSLLTGFALAFARAIGEYGSVVFIS
                     GNMPMKTEIAPLLIITKLEQYDYEGAIAIAAGMLLLSFVLLLTINLMQRWMLQSRQTE
                     AK"
     gene            complement(66011..67057)
                     /locus_tag="PLE88_00255"
     CDS             complement(66011..67057)
                     /locus_tag="PLE88_00255"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOM74930.1"
                     /translation="MKINKKTYLISVLWMMILGIGNWAFSKEITILNVSYDPTRELYQ
                     EYNKAFADYWKVKTQDTVIIQQSHGGSGKQARAVIDGLPADVVTLALAYDIDAIADKG
                     NLIDKQWKSRLPNNSSPYTSTIVFLVRKGNPKNVKDWDDLTRSDISVITPNPKTSGGA
                     RWNYLAAWGYALAKYRAAHPDEENASKADAYARDFVSKIYKNVPVLDSGARSSTTTFV
                     QRNIGDVFLSWENEAFLAIKEFGADKFEMVIPSLSILAEPPVAVVDKVAQRKGTLAVA
                     QAYLEYLYSEQGQEIAAKHFYRPQMKTVAQKYKNLFPEIKLFTIDEVFGGWQAAQERH
                     FNDGGVFDQIYGVK"
     gene            complement(67098..68513)
                     /locus_tag="PLE88_00260"
     CDS             complement(67098..68513)
                     /locus_tag="PLE88_00260"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOM74931.1"
                     /translation="MKDDSTVLAFLILVFTLFLCTAHADSPQDTKTLEERLNQLEQKQ
                     IDVERQLEMERQRTAYLAEQLAKTQAELDRLMQAQGTIASGAVVLQDNSSGQPDERKQ
                     IEQIVDQRLEMRKKDFAVPQWLEKMQIFGDFRYRYEWTDDASKTADRNRNRIQTRIGL
                     TNAVNEEFSYGFRLASGNSEAPLGEGSPTSNNQDLDNAFSSKNIWLDLAYVDYHPAGI
                     GGLNIYAGKIKNPYYMVGNSDLMFDTDITPEGIAGVYSTSVSSTIKVFGTVGGYWLEE
                     RQTEADSSLWAVQAGTTWQFGHSDEYLTAGGGYYDFGNVKGREGLGANSSQFYGNKAI
                     GGYYVSDFDIIQGFAEIGVPMGSLPLRVFGDLAHNTGAESGQNNAWLAGASIGKAAQK
                     GTWSFAYNYRDIEADALLGVLTEATFGGGGTDLKGHKYSFSYQLAPNTQIGLSYMDAK
                     RNRSGKTTDYDVVLADFTIKF"
     gene            complement(68588..69052)
                     /locus_tag="PLE88_00265"
     CDS             complement(68588..69052)
                     /locus_tag="PLE88_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOM74932.1"
                     /translation="MKISMKTDYAFRALFTLVEHYQRGPIPIRELARRNNVPKRFLEH
                     IMLEMKAKGWVASTMGKQGGYILAKPPHKITAGEVIRHFDGVLAPIGCVSLRHYEHCD
                     QRPVCRFRSLFLDVRNYAAKRLDHTSLADVFQNMLIQDDEALAAQFTDGAGI"
     gene            complement(69136..69600)
                     /locus_tag="PLE88_00270"
     CDS             complement(69136..69600)
                     /locus_tag="PLE88_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74933.1"
                     /translation="MNTKRNSGFILMIVFVLIPLMGMAMAIFSANSKILGYQVRMEQL
                     QQHAKDACRSGLTWASVHQSKLNDLEKTVILTVDESPVQIRCSIALISQNEIQKVYEV
                     IGIATDSRYDAYHREQLVVKKNSNSFIFKDIEFTRLYSFMPDFVFVFIVKIS"
     gene            complement(69648..70454)
                     /locus_tag="PLE88_00275"
     CDS             complement(69648..70454)
                     /locus_tag="PLE88_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM74934.1"
                     /translation="MKANVHRKKTAFTLVELLVVISLIAMLMSIVLPALRATREQARK
                     VLCMANLSQWGELFTLYAEDHDGRFFAGYYSYADPSGVLLQSRSADLWPYAMQAYYDT
                     PKLKFCPAARQTYGTGKSTSQSGQAETILSGSYGLNGWVCDTPPEVIQTEGHDTQYSW
                     RTIDVVGGYKIPLFLDALWYTGRPEDSDVPPQSRDYLLNTQWAAQPDNQMQRFCVDWH
                     RNMLNVLFPNGDVSAVSPKELWRLKWHKGFDTSKPLPQWPEWMVNFKDPD"
     gene            complement(70458..70952)
                     /locus_tag="PLE88_00280"
     CDS             complement(70458..70952)
                     /locus_tag="PLE88_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74935.1"
                     /translation="MKLKGYTIMEMLAVLAALVVMMALLTKPMHQIISELPRSYKDYQ
                     TLTQTLHMLKHLQEDVEHSDHLSVIEMDPRISSNLLILKQAGGLVSYCLADGKVVRQS
                     NLPRDRKEDVWELPNVYLKWIVWKNEQGPIALELAVWNERPILGQSKQKFQQTHVYFI
                     KKEI"
     gene            complement(70949..71353)
                     /locus_tag="PLE88_00285"
     CDS             complement(70949..71353)
                     /locus_tag="PLE88_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74936.1"
                     /translation="MKNFSFRFHAHKKRFGVLSFELITAISVLTILIGALAAVGGTFK
                     KANDLRWIRHTLLAAGQAQMDALDITGKPIAKDTFACLWPNVDCRIDITEGEGQWQGL
                     QRIQLTLSAKSGRKTVKTSLVRYVAAQKELKQ"
     gene            complement(71350..72747)
                     /locus_tag="PLE88_00290"
     CDS             complement(71350..72747)
                     /locus_tag="PLE88_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM74937.1"
                     /translation="MGLILLPFAMAGMILSAIDFDRGAIAGITDEAFWIVVMAIGLFC
                     ASIVLVRLVPTAGHLETPWYKSFAIIVIILFKYLILLALLTAVFHILGIVLFILVVVG
                     IVRYKLTCRYTLALDVLSAVRMSMRQSLPLPMVLNAAAQGQKKRRSRIFRDIAEWLTQ
                     GWTLSEALTRGYPKCPPELLAAVRCGEKMNQLPNAIETILADITERVDNYRRVRPVHP
                     WYPLLVMIAAVFFVLGLAVFIIPQFAQLLEDLSDHQTALPAVTQILFQIVQFIMGPRG
                     FNAAVIAAVILLIVLRCVFSSIYRRRSGRLSLTNRLSDWFKWHLPVIHWFEKTRAHLQ
                     LIEMLKVGLQAGWPVNTVIQNALDLDMNWCFRKRLEQWLSRVEEGQNIAKSAQQCGFD
                     KALVWSFDESINKGNTPQILDALEHLYRSRYHYRINLMNAVCCPLIIVGLGCFVGFVI
                     AGMYLPMVKIIEMFL"
     gene            complement(72821..74020)
                     /locus_tag="PLE88_00295"
     CDS             complement(72821..74020)
                     /locus_tag="PLE88_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM74938.1"
                     /translation="MALFEYRSCTKAGRLMTGTIEAANIDQARISLEELGLQVTELEQ
                     AKPAPAPHPVGRSEFMLFNEQLASLTKAGIPLERGLRELAHDAGSGRMKSLLNSIADD
                     LEQGIPIDKAIEKRQKHFPPLYNMIFKAGIETGRLHEMLANLNRHLQVEHRTRRIVIE
                     AISYPLMILLIAAILVTFLFTMVVPAFAELMWDMSDGRLGLPMLTVFLLNLAEHIWSF
                     WIGAGILIGGCILFWIVLSASPEGRQIKEKVLDNIPLLGRVYRNGLMSRFAESLSALI
                     KAGCTMDTAVELAGQSSGSERLKQDCVLLAGQLREGFNVMEAGLNCSVISHLFLYSVQ
                     LGSQRNELRENLDGLAQLYAAKTYTLQSQLQAVLMPLLIILLGGIIGLIILSLFLPMV
                     KITQVLM"
     gene            complement(74024..75256)
                     /locus_tag="PLE88_00300"
     CDS             complement(74024..75256)
                     /locus_tag="PLE88_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74939.1"
                     /translation="MSKAFFLLLILTVCVCSLADAPNQAEPKPNILPARGPADPNILQ
                     WISASAMKIVELTPAADIGRPMFHLLPLKTDLTNEASLPIYLQVFTEDPNQPEKNPDF
                     ELIEQQISLPAEQLDIKAVATELERCRDRLQLLQKASICRNIEWPALDSHSPINSYPV
                     SAVHPDESALFTLTPKEYLDFLMDIYRYGRLTALKARYHIVRREYNSAAEWLQSGLSI
                     SRQMVCRSNPQLAMMAAANAAAMLTQIELWIQTPGAPSLYRSLQDLPMPFLVADSTDA
                     PDRKKTEQFNPMMEMLSDGTAAIPDVNEIRTQQETLPVYTSRQAEQMCQQIDRFIAVL
                     ECIEGLRYYASVYDGLLPNSLGEITEIRLPCDPVTKRPLIYYRQEDAYILQTADVLRE
                     PMPSAFRYKIRLMSHAKE"
     gene            complement(75287..76435)
                     /locus_tag="PLE88_00305"
     CDS             complement(75287..76435)
                     /locus_tag="PLE88_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOM74940.1"
                     /translation="MNQTSQPNVAALVEQLLSEAIRYGASDVHFEPTAKSLLVKFRLD
                     GSLNTVETLPKAISENIIARLKVLAGLLTYRNDIPQEGRMEGFKDISVASEAGTITDL
                     RLAVFPTIYGQRAVVRIFYENRGLMQLDELGLNDFIESALRKFACRNQGILLLTGPAG
                     SGKSTTLAALLRYILQQFPGNSIVTLEDPVERAIEGITQVQIEPQGQMSFPMALRSLL
                     RQDPQVLMIGEIRDAETARIAVEAGLTGHLLMTTMHSGSCSGALLRLLEMGIEPYQVT
                     SGIHAVLNQRLVRKLCDSCKRQLPDGAYEAVGCSRCHMTGYHGRVLIAEMVELDGMLR
                     KAILDRSDMETLDAILASRGHMNLMHDGRRLVSRGITTEDEIRRVCGV"
     gene            complement(76600..79263)
                     /locus_tag="PLE88_00310"
     CDS             complement(76600..79263)
                     /locus_tag="PLE88_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOM74941.1"
                     /translation="MKGKSVLFLFFFYLAGSLSAAVWYVDSDVAPGGDGKSWPTAFNS
                     ISAAIAAASPLYMICYAPSDQIWVKSGTYNLTSELTLNKVVVMYGGFPNAMASPTLDD
                     RNPAVYPTIINGSNSVRCMKITSYSIIDGFIFQNGRASSGGALYIESPTYSCLGFDFS
                     TTLQNCRFLNNVATVVGGAVYDLRSNLVIKECLFQDNSADNGGAIWQWQTNSRIEKCI
                     FKSNSSTAGGFGGGAILGDYQTYGSIINCLFVSNSSASYGGAISYHQAWPSIINCTFA
                     DNTAAYAAGGGALYTNTSSPTLRNCIFWGNSPNQIVDYYSSPGPTASYCDIQGGFAGT
                     GNINANPLFTGSGDYHLTASSPCIDAGSDFDAPAEDLDGKPRPLDGNGDSIAAIDMGA
                     YEFEYINVDLRITSISLNPKVPHVGQPVAVTVTIHNAGTTNAGSFYLDWYADRASLPS
                     PGTVGDRWKRFSSLAAGASDAMMVTYTYATEGIKRMYAQVDTEGEVEETYETNNIYGP
                     LTTWAMIGVLLDFNVIHDQHNAVIWFGGDDRVDKRNVGVGQSITLTRPANVTSAGFRF
                     DDPFDYYTNPEGHGHAVYLIMYARKADGSILAPSVKLVPSNFTGGWVMMPFSNLWLEP
                     NQTYIFTCYLYQGEYNEFTSGVCGRWDNPWPSSTGYAAEVSGQPANMELWSNWNTHPG
                     DFNLQLTGQYVERYPGDINDDRSVNLGDLSVVADNWMVSDCIMPNWCENSDINWNSRV
                     KLEDFSVISRFWRAVYHDYDALNRAAVVTLESQMSTASIYGSDGMEFNPGTYFVYKTS
                     AGRYGKFIVEQLTPSENHKLTLGWVTYNADGTVYSSGTGLIVRGTYNCDLDVGLETSV
                     GADFQWVQATSTVRYLYPKNGAKFKLMYRAP"
     gene            79517..80092
                     /locus_tag="PLE88_00315"
     CDS             79517..80092
                     /locus_tag="PLE88_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM74942.1"
                     /translation="MAMNPETLRLAFELTGSTAAEKSQMWILSAMQYYGPAIVSMLWR
                     ILGREEDVCDAYQETFLHLAYLPQQQKPANLRAYLFRTASNVAISMLRRRQIEKKYQE
                     QIQHKAIPSSPDPAGFLDAMDLQQQLREAIAHLPDYLSDVVVLRDLAELPYPQVAKIL
                     GITTTAARVYRHKAVKLLAQWMSKLDKRDDL"
     gene            80089..80598
                     /locus_tag="PLE88_00320"
     CDS             80089..80598
                     /locus_tag="PLE88_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74943.1"
                     /translation="MKTIRLNNLCRVLQQRKVGLFDSFRQHVQHRLIEHMANCPRCRK
                     RLGLASRLEIALAMIKSQPHHIDLLQRANTQTLAVFAHALRYAPKSQTLRISRPEPIW
                     AVKNRSLIDRLLNIAACLFVVLMMRSGIFHSLTSYKEQSEAVIHNYYARNLDSNMFNE
                     IFPNNDTLA"
     gene            complement(81089..82111)
                     /locus_tag="PLE88_00325"
     CDS             complement(81089..82111)
                     /locus_tag="PLE88_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74944.1"
                     /translation="MTAKHSRIILFLCLCTFVRGDFSARQKVIKLMENAGLEKYLKAP
                     VTITPKAPGLLLIADQGVFAVQHELLTPAHSAEPNVVIQDGLCIIPTADKLTVVTHGW
                     LDKGEDDWPSQMAEAIAARIDPNEWVCASYDWKGGSVVFTSVQAAQYARDIAGPRLAA
                     AVLKLDRPFKHIHLVGHSAGSWVIHSAARQIARKYPNTQFHLTFLDAYIPDKWEAEQL
                     GQVFELPECQKSQYWGEHYYTRDITRKVTEHNLKEAHNIDISALDPLIVEHEFPYRWY
                     MATITGRYQRWDERDEPVATRCGDMDYGFLRSLEAGLENWNLSRQLPVGNPALKIIVK
                     QLEQRQ"
     gene            complement(82127..84955)
                     /locus_tag="PLE88_00330"
     CDS             complement(82127..84955)
                     /locus_tag="PLE88_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74945.1"
                     /translation="MRLQNYLFAAVFAAGFTVSVVFSQNTEMYYCLPLRQIEIEGQWP
                     DKVLSSPAPLDWGLRQQIKYYLPYAAGDRQEEIYIDFGEFIQSGGQNRPDFWRIQSVG
                     QLLDNASVAIKSSHSLPITGIIFLPTANLKSMSAVRFRISKEPVNAEQTSAKRHFFTA
                     MKNHYTLLQSCGFTGTEWFAYRAKQAQKILDDQQCSGVSQQGRLLPEQDPFEDIMLFF
                     TGRKAVMENIQLDRTLQTRFNEPRVVDIAGIEGITTSAIDWNKQNEGIVFKKDALARF
                     IPADQHALFYPTFKSMMHLLDRLKGTWYLPDEFQTQNIESYERQMCVWLDGWSRFWGP
                     KTIASVALTGSDLYWKAGTDSAVLFDAPIARLVYSNTESKQQEALEKVKDAKKLTGMV
                     SGIPYQAVVSPDRTVSSYLARLDNAVIVSNSLVQLEKIIKTAQGKQPSLAEAGEYAFF
                     RSCYPADENRQTAFLVLTDAAIRRWCSPRWRIGAARRMLVSTVLSQLQAGYLDNQDRF
                     DLAAAQTTALNWAPDIGDITITNAGITSSVYGNLRFMTPISELAIEKATERERNQYNR
                     FRSSYQSRWRTFFDPVAAAVLLESDTIDIDITIRPLIADSEYRQLMQIGGKNFLQPDD
                     GDPHAKTILQAILAIDSDSEPVRLAGGFARPMGGPEWINTHPLSWLGRWITIYAEEDS
                     FWGELAGILSGHTLDYEFFEYLEQNINRLPLAAAMDVDSPSQLTIFLVGLRAFIEQTA
                     PNMTVWETLNWQQKSYVKITFQYEQAKQNPPSLYYAALPNQLIITPHEKLLQQAIERA
                     SVSARQNGTKAPVQWLGKNLAVQADEKAWKVIEIIASQSWNRYLQMQSWRHLPILAEW
                     RMRKGELSETDFHLRYWHTRLTCPGGGQYVWNEQFHTFESTVFGHPGQPRMPDDLLTP
                     MQAVQQVRLGITFEEDGLRARTVMKQKK"
     gene            complement(84957..>85504)
                     /locus_tag="PLE88_00335"
     CDS             complement(84957..>85504)
                     /locus_tag="PLE88_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOM74946.1"
                     /translation="NKVYYKPFILFANIPFAIAVFYAAAMLLGAWFVKCSRGKGGPLP
                     AAIVMASLILLWILLSEEIYWYFRMQAPVLDSWKFLSRMYISVSWAFYAAFLLVIGFV
                     VRSAWIRYLSFGIFAILLGKINLDMWVLGTEYRIATFLTTGLILVGISFLYQFLKKKG
                     FFESNQSRIVELAKVSQKEGV"
BASE COUNT        22810 a        21454 c        19840 g        21400 t
ORIGIN      
        1 cgccgcatta cccgcgcggg gcttttgcac aggcatggtc tgttgccgag gtcctgcgcg
       61 catggcaact ggcacaagac atataaacca gcagcctatt aataagctgt gtcgaacgag
      121 ctgcaaattg gtctatggtg tattttaaga ccgatattca aactacgctt cttgtttgat
      181 atttgaattg tcctttttgc cggcatgctg acggtatttt cgatccatca gaaaaattac
      241 ttttttgaag atctctgccg gcagtttttc ccgcagcagt ttacgggctt caatcatact
      301 ggtgcgctgt gtcaaatggg taatcagtat aacctgatta ttcattctct ccagcagcgg
      361 tccgaactca tcaatatgca tatgccggcc ggcatcagcc cgctgggtat gctcatctgc
      421 aaaaaaagtg cattcggcta tcagtatccg gctgttggaa acataatcca gctgcgaaaa
      481 atcaacatag gaagtatccc ccagatagct gacgatcggc acttcgacag ggttatcaat
      541 aacaacaccc tgctttttca gctctaccag ctgaggtccg gtgagatgat ggtattccgg
      601 cttaagcttc ttgcgtgttt cgataactga aaatccgacg gcctgacgcg tgtgttttgt
      661 tggaaaaacg cgggccacca gattaggctt caggcggtac tcatctcccg gctgaaccgg
      721 caccagattg gcaggtatac gcgtcccatc cagccgcccc catgcatcaa gaatctggcg
      781 aatcgattca atggtattgg ggggcgtcag aacagttcct tccggcaggc cgcagaattt
      841 tcggtgagac agatagtagg caataccggc agcatgatcc atatgaccat gcgttaagag
      901 gacgtggtta attgagatca attgtccggg ggccttgccg atgtcaaagc agacatccag
      961 ctgcggcata cctactacgg tctcctcgcc ggcaaccgaa tagccgatga tctctaaatc
     1021 atcaattttt aactttgcta aatgatgact ggccattgtg aatcccaaca tccaaaacag
     1081 catagtagaa aaatagagtc cagctgtcaa cttctttttg actgcttgtt cggaatcaat
     1141 actgttgtta tgataaagat atgcctgaca aacgcagcca tcgaggtccg catccggacg
     1201 atgcggctct gtttacagat caaagccaat tcatccttgc taaggcgaca gcggatatga
     1261 gctggcttct cagccgggga tatgctgaaa aatcatcgct taagctgatt ggcgaccggt
     1321 acaatcttaa acagcgccag agaatcgcag tcatgcggtc agcctgttcg gatgctcagc
     1381 ttcacagacg cagggcaaca gaatttaaac aagagaaaat caagggacaa ccgttgattc
     1441 tggatggata taatctgcta attacaattg aagctgcatt agggggcggt attctgctta
     1501 taggacgcga cggctgcatt cgcgatttgg caagcataca tggaacttac cgcaaggtcg
     1561 aggagactct cccggctatt ggtattattg ctgacacgct aaaccgcttg gaaataacca
     1621 atgtaacttg gctactggat aggcctgtat ccaattccgg ccggcttaaa aatatcctca
     1681 tgcagtttgc acaaagccgt atggtcaact ggacagtaga gctggtacaa agccctgaca
     1741 aaaccctcat cgaatccggc tggattatcg ccacaagcga cagtgccgtg ctggacagct
     1801 gtctgcaatg gctgaacctc ggaaaaatag ctatcgaaag cttaaagccg aatcatagat
     1861 tgcgtctttt tgacatgaga atgcccgaat cttcctctga cactatttga ccgcgtgaac
     1921 agctcactgc gtccaattga aaacaagcca tcatcttctt tactgtgaaa gtacaaattt
     1981 tcctaaaaaa gccatatttt aatccaattt tttcaacaca ccttgcttta aaaggcgttt
     2041 gtaaatatga tgttgcaagt aaagcagaac aggttgttga gaaaaaatcg tattggccgt
     2101 tgctcaggaa acatcttctg caagtctgat cgaggcggct cggacagaca aaatggcatt
     2161 ttctaccctg tttcgccggt attacgacga aattttccga tattgcgctc gccgtcttcc
     2221 tgatcgggat aatgccgagg atgcagccag tcaggtgttt ttgaaaatgg tcagcaattt
     2281 cgatagtttt acgggggatg aagtggcctt tcgctgctgg ctttttcgca tcgcatgcaa
     2341 tgaaatcaac aatttttatc ggcaaacagg ccgccaaaat agactttttg aaaaacttca
     2401 gcatgaaacg gcctgctcta atgcaacaag cactggcttt tcagataatg aaaatcaggc
     2461 aaaactgcaa tttctccgtg ctgccgtcag cacacttaaa cctaattatc aggacatttt
     2521 gacactgcgt ttttttcagg gattaacaag cgaacagatt gccgaaattc tcggcttgaa
     2581 tccggcaacc gtgcgaagcc gtctgagccg gtctttgaag catttaaaaa aattataccg
     2641 tctgcatcag cagaaaatcg caaaggaaat cttttccgat gacgcctgat gaacacaatt
     2701 tcgaggaatt tgtcaaaacc attcgctttg atgaccagcc cagcagtgag catcgccagc
     2761 gattggaatt tcagctcctc caacgatggg aaaacagaca aaaagaaact ggatatattg
     2821 agccggcagc tctttatttg aagcgtctgg ccattgcggc cgggattctg ctggcaagca
     2881 gtgtattatt ctggacaatt gataatctgt ggataagccg accgctggcc gttcatccag
     2941 attctaaaat gatagaaacc atcctccagc aggaacaacc atcggaagca gagaagccgc
     3001 atttgctggc acagattaac cacgtatggc atctaatctg ccgtcaagat tctgaggcat
     3061 tggtcagtat cctgcagaca caagaaaccg cctatgcaat tcggtgctgg gcagcgaaat
     3121 atctgggaca attcggcaac gaaaaaactt tggaactgct caatactgcc atcgaaaccc
     3181 ttcacatcag tgattccaat gagccgctgg tcctcgcagc ccgcaccatt cgccagcgtc
     3241 ttgaggcgtc tgaacccaac accccaaact aatgtgccgg cgcagtaaaa caaacaccgt
     3301 ctctaagccc cccgcttaga aagccgatgt ttgtttttgt tgggaattgt gctcctcata
     3361 ccggaccaca gcccccgccg ctgaaggcag gcaacaactg ttattggtgc ctatccgatt
     3421 gactgctctg ccccattttt gaccgaaaca agtcggcggc ccgcagagca ttttcctcgg
     3481 cgaaacgaat acagatatcg attccgctgc gtatggatga tgcccacgca tggcaatcga
     3541 tgccggcatt gccgccgaag gcctcttcag caggcaatct gctgtgctgc ccttcggact
     3601 gtgtttcaga caatgccgct gctgaaaccg gggctaaaaa atagactgcc gtaaggaatc
     3661 ctgaacaata cacaatcagg gtaaacagga ttttgcttcg ccaacggccc atcagagttt
     3721 ctcccgcgga aaacatcagt tttcttattc tgccggattc gaatccagcc ccgtcaccaa
     3781 ttaaaatcta caaaataaaa ggtgctccgg caaataaaga aaaatcgtat tcttatactg
     3841 ataagcgtaa ataaaagact ggtacggccc atccaagcgg acaatctttg tccactaatc
     3901 acacagccga aaccgttggg cttcattctg ccaaaaccag ccctcataga atgaaatcct
     3961 gcaagatgtg ttttgtctgc ggcggattgt ttagagttcg tccaaacagg cctttaattc
     4021 cctgcaaacc tgctggattt ccttctcggt cagattgttg tggaaaggca gtgcaatcgt
     4081 actgctggaa atggtatccg ttacggggaa atcgccttct ttgaaaccga atttttcagc
     4141 aataaacggc tgaagataaa ctggcggaaa ataattgcca acctggatgc cgcgtgccgt
     4201 cagcttttcc aacacgctat tacgctgctg acgggtgtat cggtcggcca gacgtataac
     4261 aaaaacaaac cagctcatcc ggcagccggc tggagtttgc ggcacaatca ctcggtcgtc
     4321 accagcaaga tagtgacgat acatatcggc aacttcgctg cgcttgcgta caaattcctc
     4381 aatgcgagaa agctggacaa tacccagtgc acagttgata tcgcacagcc ggaagttgta
     4441 gcccagccgt tcatgcgcca gccagccggg gcctgtcccg cggccctgat tacgcaaaga
     4501 ccggcaagct gatgcaagct cgtcatcatc tgtcagaatc atcccgccct cgccggttgt
     4561 catctgctta ttcggataaa atgcaaaaac gctcatcttt ccaaatgtac caactttctt
     4621 gccgttcagc tgagagccga gcccctcgca gctgtcttcc aaaacagtca gcttatattt
     4681 ttgcgcggct gcgcaaatgc agtccatccc tgccggactg ccgaagactt ccaccggcac
     4741 tatgcctttg gttttttcgg taatggcggc ctcaatcttg tccgcatcga tattcagagt
     4801 ttcagggtcg atatctacaa aaaccggctt ggctccggcc atcagaatga tattgacggt
     4861 tgcgataaaa gtaaaaggcg ttgtgattat ttcatccccg ggaccccacc caagcgcctg
     4921 tgtgcataaa aacagaccgc ttgtgccact gttaacagct atggcatgct tgagaccaat
     4981 ataacgctca aaggcctgtt caaattcgcg tagtttaggc cccagcgaca aataaggact
     5041 acgcaacaca gcgcaaacgg catcgatttc ggcttgagaa atatccggac gagacagggg
     5101 tacttttatt tccatcgcag tcggccttta tctaaaacat attgtaatct accataaaaa
     5161 gttgtaatgc tatatatttg ccttattgct gtcaagattt atcttatttg ccggccaaat
     5221 accgtttata gaaatcattc gaaatcgaaa tcgttgtggt ataatagcaa tctggttcca
     5281 tcatgaaaac acaacggctc acaataagaa ctttctttgg gattatcacg gcaggattga
     5341 ttgtcagcgg cttcagttcc tgcagagaag attcttccct gtctggtccc agcggttatc
     5401 cacctgcaac agaaaagtct ctttctaaat cggccgaacc caatcagccc caagtctggc
     5461 gccggccccg ctccgatgag cgacgtcagg aacggctcga gatggttcaa tggatacgcc
     5521 gcggctatgg gctggacgag ccgaggattt tggaggcaat gcagaatgtt ccgagacatt
     5581 ggtttgtacc gccggcccag cagccgtatg cgtatcacga tacgccgctg cccatcggtg
     5641 aaggacaaac catttcacag ccatttatcg tagcggcaat gacgcatctg ctagccctga
     5701 atttcaatga ccgtgtgctt gaaatcggca ccggttcggg ctatcaggcg gctgtactca
     5761 atgaatttac cccacaggtc ttcacaatcg agattatcga atcactcggc aagcgggcta
     5821 tccaaacatt cagagagcat ggctacgata cgatttgcgt taaaatcggc gacggctaca
     5881 aaggctggcc ggaatatgcc ccctttgacg ccattattgt tacttgcgct ccagacagca
     5941 tccctccacc cctgctggac cagctcaaag ccggcggcag aatggttatc cctgtcagca
     6001 gggagacggg ctatcaggaa ttgattttag ttgaaaaaga cgcacaggga tctgtctcca
     6061 agcggtcggt tatgccggtt cgttttgtgc cgctgatcag aaaaggaaac tgacagctcc
     6121 agctggccaa ttttctgccc cattgggctc atataaaaga gagccgactg ggaatcctca
     6181 ttaacaaacc ctctatttcg cctttttata ttcagttgac cggaggcgct tatcgaacaa
     6241 gaactgcctt tttgagtcgg atatcgccgc tggaagaccc gatttgaatt tcaaactctc
     6301 ccggttctac ggcataccga ctcagacgtg aatcataata ccgcatatcg attgacggct
     6361 tcaggacaaa ttcaactgtt ttctgctgcc cttttgcaag agatatccgt tcaaatcccc
     6421 gaagctgctt cagcggcatc ggccatctcg actgcacatc ccgaacataa agctgcacaa
     6481 cttcttcacc atcgcggttt cctgtgtttt tcacaactgc cgaaaccgta atcgtttcct
     6541 cggggccggc ttcatctcga ctcaacttta attctgaata ggcaaattca gtatagctta
     6601 gcccatgacc aaaggggtat aacggctgtc ctgtgtagta tcgataggtt ctgcctttgc
     6661 cggcggccat tgcataatca gaaaaatcac ccaattcctg cgtagaggca taaaatgtta
     6721 caggaagacg cccagccgga ttataatcac caaacaacac ctctgccact gcatcgccgc
     6781 cgcgctgccc cggataccat gccatcagga ttgccgggag tgtctggtct aatccgtcaa
     6841 atgctattgc acttccggcc atcagcacaa gaataaccgg cgtgccggtt tgctgcatgg
     6901 ctttaattgt ttcgagctgc accgccggca gttcaatctt ggtccggtcc ccgcggttga
     6961 aaccgtcggc ctgcagtttc atttcctcgc cctctatatc ggcatcaagc cctccgacaa
     7021 aaatggccac atccgccttt gcaacatctt ccagcatgcg gccggatggg gcagaagaat
     7081 ccggcggcag ccaaacaaaa tgaattcctg catccttgct ggcctcataa tattcaatcg
     7141 tgatatcgca tcgctgatcc ttttccagag acacatcggc ccaatagggc tgctttgcag
     7201 gctcagacca gccgtcaata accttttttc cgccaatata taaccggcag ccgtcgtcgg
     7261 acataattcc cagcttatac acgccgctga cggggggttg aagctgacct gtccaacgta
     7321 ccgaaaaatt atccgcttcc atttgcttgt caggcgctaa aatcccctgt gcaacagctg
     7381 tcgccgttgg ggaatccaat ccccagtcca tgttgataac ggtgtcgatg cgagtcagga
     7441 ccggtttgcc ttccaaatga atgccggagt aataatcccc ccgcaatccg gacacttccc
     7501 ggcccgcttc atcgcggcag aataggtatc ttgaatccac cggctgatag gcagttcgct
     7561 cgacaccttc tgccagaggg catccaagcc agtacagtat ctctacttca ttgcctgctg
     7621 ctttccacag tccttccaag actgttaccg gatgagatgc ctgaccataa taattgcccc
     7681 gcaggactgt tgtgctgtcg gcattgggac ctacgaccgc aatcgtcttg atccgctttt
     7741 tatccagcgg cagaataccc gtattcttca gcagtgtcat cgatttacgg gccatttcca
     7801 atgctgcctg actgtgtgcg ggacaatcat tgaccgaata gggaatctgg gcatacggca
     7861 ccatttccgg cggatcgaac atccccagtt tcatccgtgc cagcataagc cgccagaccg
     7921 cccggtcaat ctgtccttca tccatcattc cagcctgcac agcatcgagc agccagcgct
     7981 gatatacctg cccgcattca agatcgcatc ccctgcgaag cccaatagca gctgcttgag
     8041 cggcattctg ggcaatcttg tgattggcat agatatcctg aatggccccg cagtcggaaa
     8101 caacataccc atcaaacttc cactgctgcc gaagtatatc ctgcaggagc atccaactgg
     8161 ctgaggcgga ttcgctgtca acgcgattat aagcccccat aactgaataa gccttggcgg
     8221 tggccaccaa atcgtaaaag gcaggcagat aggtttccca caaatcccgt gtattgacta
     8281 cagcattaaa atgatggcgg tctgcttcgg ggccgctgtg aacggcaaaa tgcttagcgg
     8341 tagccactac cttcaagtag cgcgggtgct ctccctgcag cccgcgcaca aactgaatac
     8401 ccatctgtcc ggtcaaatac ggatcttctc cgtaagtctc atggccgcgt ccccaccgcg
     8461 gatcccgaaa aatattgata ttgggcgacc agaaagtaag ccctttgtac cgcccgtaat
     8521 cctgctgacg gacaaactca tgatgcttgg ctcgtgcttc atcactgata atagaggcgg
     8581 ttttgtacat catggcgcgg tcaaaacttg ccgccattcc gatggcctgc ggaaaaactg
     8641 ttgcggtgcc cgcccgcgca actccatgaa ggcattcatt ccaccagtta tagggcggta
     8701 tgccgagccg ttcgatggcc ggagccgcat cacccaactg cgatattttc tcctcgaggg
     8761 tcatccgctc aacaagatcg gatgcccgga tttcaaagtc aacagtaaca tcctgataaa
     8821 ccggtttggc gctgtcagca gcccagcaaa tcccttcagc aatcatcagt atccatacga
     8881 tccgctgcct attcatatac cattttccct ttcttcattt ttattctctg gatacttcca
     8941 gccaattcgt ttttcccaaa tgccataatt ctattatccc tccttttggt atgcaaggca
     9001 attcacagcc gacctgcaaa acaagtacaa agcagtcctc ggattgatca aactaaaatc
     9061 cttgcccttg cagattcttt gggtataatg gaggttttaa gacagtaaca agatgatttg
     9121 ccggtaaaga caaggataca ttgatggaaa acggacaata cgactttaac cgtattgaaa
     9181 agaaatggca gaactattgg ctggtaaata aaacattcaa aacacccgac acatctgcca
     9241 aaccaaagta ttatgttctg gacatgttcc cttatccgtc gggggcaggg ctgcatgtag
     9301 gtcatccgga gggctacaca gccagcgaca tcgtcgcccg atacaaaagg atgcgcggct
     9361 tcaatgtact gcatccaatg ggctgggatg ctttcggtct tccggcagag cagtacgctg
     9421 tacagaccgg cacgcacccg gccgtcacca cgcagaaaaa tatcgataat atgcgccggc
     9481 agattcagtc tttgggcttt tcctacgatt gggacagaga agtcaacacc actgacattc
     9541 actatgtcaa gtggacacag tggattttcc tgaaattcta caacagctgg tttgatgagt
     9601 cgctgcagaa ggcccgccct atcagcgagc tgcccattcc tgacaatctg accggtcctg
     9661 aacgggaaaa atatataaat gaccgccgcc tcgcttatga agctgaagca cccgtcaact
     9721 ggtgtccagc tcttggaaca gtgctggcca atgaagaagt cgtcgggggc gtcagtgaac
     9781 gcgggggcta ccctgttatt cgcaaaccga tgcgtcaatg gatgctgcgc atcaccaaat
     9841 atgccgagcg tctgttggaa gggcttaagg acttggactg gtcggagtcg attaaaaaac
     9901 tccagcagga ctggatcggc aaaagcgtcg gagcagaggt agattttaaa attgacggaa
     9961 gcagcgacat catccgtatt tttaccaccc gcccggacac actgttcggg gcaacctata
    10021 tggttatggc gccggaacat aaactggttg aaaaaattac aaccgctgca tacaaagagg
    10081 cggttgagac ctataagcag gaagctgccc gcaaaagcga cctcgaccgc acagatctgg
    10141 ccaaggaaaa aaccggacag tttaccggtg cctatgcagt caatcctgta aatggacaaa
    10201 aaatcccgat atggatcagc gattatgtcc ttatcagcta cggcaccggt gcaattatgg
    10261 ccgtgccggc tcacgatgaa cgcgacttcg aatttgctac caaattcaac ctgccgatta
    10321 ttcaggttgt tgagccggat gatccagaac aggcccaact ctgccgtcaa ggcaaacttt
    10381 gctttacggg agacggcaca gccatcaact cgggcccctt tacagggctg cggacacccc
    10441 aattcaagga acaaattacc gcttggctgg aaaaagaagg gctggggaaa aaagccgtaa
    10501 actacaaact gcgtgactgg ctgttcagcc ggcagcgata ttggggtgag ccgtttccga
    10561 tactccatac cgaagacggc cggattgttg ctctcgacga aaaccagctg cctctggcac
    10621 tgcccgattt ggaagacttt aaaccatcgg attccggcga gccgcctctg gccaaagcaa
    10681 aagagtgggt taatgtaaca ctgtcggacg gcacaaaagc aaaacgggag accaacacaa
    10741 tgccccaatg ggcaggaagc tgctggtact acctgcggta tatcgatccc cataatcctg
    10801 ccgctgcagc agattttgaa aaacagaaat actggctgcc ggtagatttg tatatcggcg
    10861 gagccgaaca tgcggtactg catcttcttt attcacggtt ctggcacaaa ctgctctatg
    10921 atctgggcta tgtcagcacg ccggaaccct atcagaagct tattaatcag gggatgattt
    10981 taggcgaaga caaccagaag atgagcaagt ctcgcggcaa tgtcatcaat cctgaccaag
    11041 tggttaatga ttacggggcg gattcgatgc ggctgtatga aatgttcatg gggcctctgg
    11101 aagcaacaaa gccctggtca atgcagggcg tcgaaggcgt acaccgtttt ctgcagcggg
    11161 tttggcggat gatcattgac cctcaaagcg gtcaggtttt ggataatatc agcgacagcc
    11221 aagccgatga agcaactgtt cggcttctgc atcagaccat caaaaaagtt accgaagata
    11281 tagacggctt cgcttttaac acagccatca gccaaatgat gatttttgtc aatcacttga
    11341 gccgtcagga ggtccggcct aaaaagattg tcgaacagtt tgttttaatt ctctcgccgt
    11401 ttgcaccgca tattgccgaa gagctttggc agcggctggg acatacccaa acgctggcct
    11461 atgagccgtg gccggcattt gatgccaatc tggccaagga aaaggaaatc gaaatggcgg
    11521 ttcaggtgtt gggcaaaatc aaggacaaaa ttgttgtccc tgcagacgcc ggcgatgatc
    11581 agattgagca ggcggcgctg gccagcgaga aggttaaaac agcaatggct ggcaagctgc
    11641 caaagaaaat cattgtcatt aagccgcgtc tggtgaatat cgttgtttag tgtgaaaaac
    11701 tacataactt tcaatggcac aagaaatcga agctaaaatc aaagttgagg cgctggagcc
    11761 gacagcccag cggcttacag aattaggagc gcggttttct gccgtacagc atcagcacga
    11821 tgtttatttt catgatgctg atggattgct taaaaaaaga ggctgcggac tgcggctgcg
    11881 ggaggagact gttaatggaa aatctgtctt tatcctgact tttaagggcc ctcgtcagga
    11941 cagccccttc aaaagccgcc ccgaatttca gacgcaggtg tcagacagtg ctgagatgat
    12001 aaaaattctc gaaggtctgg gctatcagcc ggttctgacg gtagaaaaaa aacgcagcct
    12061 ctggcaaatg gacggctgcg atatctgctt ggacgaagta gaaatgctgg gaccatttgt
    12121 agagatagaa ggtccaaatg aagagagcat ccgcaggatc cttaataagc tggggctgca
    12181 gaataagctg catctatcgc aaagctactc ttcgatgata gcccataagc ttcaggccga
    12241 aatgctcaac cgcaaatcac agggataaat gatgaaaccg caggatgggt atatacaata
    12301 catcgtaaat ccaaaatccg gtgccagcag cagcaagcgg atggtcagcc agttccgaga
    12361 ttatttgtct gaaaagggtt ttgatgtacg tgtacaattt acagcttcgc tgacccatgc
    12421 cggccaattg gcagccgagg ctgctgctga tgagggctgt atgctggtag ccggtgcagg
    12481 cggtgacggg actcttcgag aggtcgtgca ggggcttaag ggtaccggca agcttttcct
    12541 gcctgttccc tgcggcaccg agaacctgct ggctaacgaa ctgggcttcg accagcacct
    12601 gcagacgctg attaacgcct ttgaaggcaa ctgcataaag ccgctggacc tcgggatgat
    12661 taatggatgc tgctttactt caatagtcgg cttcggtttt gacggttcgg ttgtggaccg
    12721 catcagcaaa atccgaacag gacacattaa tcacctaacc tacttctggc cgatttggcg
    12781 aacattctgg gaatattctt ttcctccggt cagagtttcc gtcgacggca aagaaatttt
    12841 ctgcaatcga ggtcttgtct ttgtaggaaa catttcacac tatgccatcg gactgggaat
    12901 ctgcaaaaaa gccgattaca gcgacggact tctggatgta tgcatttttc cctgtcatga
    12961 ccaagcccat ttgctggcgt atgcctttaa gaccgcactc aaaacccatc tgaaaagcag
    13021 gagcaccatt tatgtccgtg cgaaaacaat tcaagtcagc gattgcggcc agccggtact
    13081 gtgcgagatt gacggagacc ccggtccctc actcccggct gatattcaca ttcttccgca
    13141 ggccgttcgg gtgattgtgc cccccggcgc caaacctgcc ggtattcgca cacgattccg
    13201 gagaataatt gactaactat atcctcttta tcttttaatg gtatccacca atacattaac
    13261 tccccatagt caaagggaat aattctgtta agtctttaat atttgtgctt tcgtgttata
    13321 ataaaggttg ttagcttcag attgggaaaa ttatgcaaaa aatatttgag ataggaactg
    13381 ttccagtcgg cttcggccag ccgttgttca ttattgccgg gccttgtgtc atagaaagcg
    13441 aatccctttg tttgcatgtt gcagattttc tggttaactt gcagagatct tccggcgtaa
    13501 aatgtatctt taaagccagt ttcgacaagg ctaaccgtac cagcattgac agttttcgcg
    13561 ggccggggat ggaaaagggc ttgtctatcc ttgaacgcat tcgcaaccaa acaggtctgc
    13621 cggttttaac cgacgttcac gaaccatcac aggcaaaacc agtcggggag gttgtagatt
    13681 gccttcaaat tccggcgttt ctttgtcgcc aaactgacct gctcgttgcc tgcggccaga
    13741 ctggaaagcc ggttaatatc aaaaaggggc aatttgtcag ccctgaagaa atgaaaaact
    13801 gcgtcgaaaa ggtccgttca actggcaaca ataagattat gctgacggag cggggaacat
    13861 ttttcggtta taatcgtctg gttaatgata tgacttccat acctgcaatg cagcaattcg
    13921 gctgtccggt tgtattcgac actacccata gtacccagcg gccgggggga ttaggcatcg
    13981 ctactgctgg aaagccggat atggctccgg tgcttgcccg agcagctgtt gcggctggag
    14041 caaatgcctt gtttatggaa gttcacccca atccggccga agctctgtgt gacgcggcct
    14101 gtatgctccc tttggataat gttcaatcgc ttgtagaaag ctgcagaaag atctacgagg
    14161 tcgtcaataa taaatgactc aaaagagata taaatacctg tttggccctg taccatcccg
    14221 ccggctcgga caaagcttag gtgtcgatat tatccctttt aaaacctgca cccagaactg
    14281 tatctactgc caacttggaa aagacgccaa gcaaacaatt gagcgtcggg attatgtacc
    14341 tatcaatgct gttcttgagg aattgcgtca aaaaattgaa gaaggactga gggcagatta
    14401 tattactatc agcggctccg gagagccgac attgcatatc catttaggac agctcatcga
    14461 cggaattcgt caactgactc aaatcccaat tgcagttatt accaatggta ctcttttcag
    14521 ccgttcggat gtgcgggctg actgctgcaa agcagatgtg gtattgccct cgctggatgc
    14581 cggcgatgaa gacactttca agaaaataaa tctgccccac aaacagcttg attttgcagt
    14641 ttttgtcgag ggtctgatgc agttccgccg ggagtatacg ggacaattct ggctggaagt
    14701 atttctgtgc gaaggcataa ataccgatga aacggcctta tcaaatctga aacgatggat
    14761 cgatacaatc aatccggata aaatacagat taatacatcc gtccgcccgg ttactcatcc
    14821 ccaagctgtt cgcgtgcctc ttggcaaact tgaacaaatc gctcgttttt tcggaccgca
    14881 tgcagaagtt attgctgatt attccaaaca cagttcccaa gacacaataa tcccggatga
    14941 agaatacatt cttaagactc ttcagcggag gccctgttcg ctggatgatt tgtgccgggg
    15001 gcttggactg gcagccgaag ccattcaatc tgctctggac aatctgctag caagccggaa
    15061 aatctcctgc gatgaacaac acggaaaaaa gttttataca gccaactaaa aatactccgg
    15121 ccgcatttct tttaccaccc tcagacctga aattcttata ggacgatgca aataataccc
    15181 cgttaattgc caaattttgc ggtttttttt taagaacttt aagttatcgg tcgataatac
    15241 agacagtaga gcgaggtgtg ttttcatttt agcactttaa aataactaat ccagtagagt
    15301 cccaaataat cgaaatagat tggggatttg ccgagacgag accgatatgg cacagataaa
    15361 aaaacagctt gttctaaata aaggttcaga gaagtttatc ttccgttatg ataacggcaa
    15421 tgaaggacag ctgctggatg cattggtagc agcggcaacc aatccgaaca ccaattttga
    15481 ttggttcgat gcggccgtgc tcagctttaa gctgacccaa tcgctgattc acgaagcaga
    15541 tgatattttg tacaaggatt taagcaaccc tgtacatacc tatccgcagg gttcttgaaa
    15601 cactcggaat acttaccctt tcgggagcag aaaaaccttc ggcagtaaaa gggatatttt
    15661 atggacaatt cagccatcat acaggaattt atcagctatc taaaatttga aaagcatttc
    15721 tccgagcata ccgccaaatg ctaccacgca gacttggagc agtttgtctc cttccttgcg
    15781 gatgcttatt ctggacatgc atctccttcc gaccatccag ccgacaatcc gtgggacacc
    15841 acgtcagcta cagcaacagc cgttcagaca aaaccggcga cgactgtgga cgaactggtt
    15901 aaatccgcag atgtcaacac cattcgccgg tatatggccg aattatctca gcatcaatac
    15961 tgtaaatcga caaccgctcg gaaactggcc acccttcgca gcttttataa gtttatggtc
    16021 aagcgaaact acctgacgag caatcccgtg gtatccataa agaccccgaa acaggagaag
    16081 aaactaccta aatttcttga atatgaacag gttattcgcc ttctgaatac tccgccggtc
    16141 aatacatggc tgggagcaag ggatcgggcg atgctggagg ttttgtacag tacaggcatg
    16201 cgtgtcagcg aactggtagc cctcaatatg gatgacgtcg atttcctcgg ggaagtcatt
    16261 cacgtccgcg gcaaaggcaa aaaagagcgt ctgtgcccca tcggctcaag tgctttgcaa
    16321 gcaattcaaa actatatcga gttccgcaat cgccgtatgt caaatgattc gaattttgat
    16381 tcaaaggtac tatttgccaa caagcacggc aaacggttaa gcacacgcag cgtccgtcgt
    16441 aaaatggaca aatacttgac cgaggccggt ctggatcctt ccatcagccc ccatacactt
    16501 cgacacagtt ttgccacaca tatgctcaat aatggagcag atttaaggag tgttcaggaa
    16561 cttttaggtc accagtcgct ttcgactacc cagatttaca cacatataac taccagccgg
    16621 atgaatgagc agtaccacag tgcccacccg ctggaacaac accagccgta aaaaatgtat
    16681 taagaaaaca aaaaaaaccg cctgacggcg gtttttttat ggctgcagtt tcgattaatc
    16741 ttttccgatg tagtttggaa ccaattcttt aatcaccgct acaatctttg atcggtcttg
    16801 tgtagcggca accgcgataa gcttctcaat tccagcccga agagactgac ggtctttggg
    16861 aatatttttc catacggcga ttttcggatg ctttgttgga agcatatctt cgccctcaat
    16921 cgaaagctct tcaaatagct tttcaccggg gcgaagtcca gtaaactcta tctcaatatc
    16981 ctctccgggc cgaaaaccgc ttaatgttat cagttcacga gccaaatcga cgatttttac
    17041 cggctcaccc atatccagca caaaaatctc gccgccctgc cccatcgcag cggcctgcaa
    17101 gaccaattgg ctggcttcgg gaatggtcat aaaatagcgg cgcatttcgg ggtgcgtaac
    17161 cgtgatcggt ccgccggctg ctatctgctg gttgaaaatc ggtattacgg aaccgtttga
    17221 gccgagcaca ttgccgaagc gaaccgtaac aaaatgcgtt ttactggtgg cgttgaggtc
    17281 ctgaatatac atttctgcta ttcgcttgga tgaacccata atgctggtag gattaacagc
    17341 cttatcggtg ctgatcatta caaagttgtc cgctccgaat gcatctgccg catctgcaat
    17401 attgcaggtt cctacaacat tgttcttaat agcttcgccg gggttcgttt ccatgagagg
    17461 aacatgctta tgggcagccg catgtataac aatttgcgga cgatacatgt cgaatatctg
    17521 aaacaccctt gctctttcaa ctatatcaca tataattgcc tcggtatgaa cctccggaaa
    17581 cttctggcga agctcccgat caataaaaaa gagaggattt tccgcctgtt ccaccaaaag
    17641 aagctgccga ggcctaaatg cacatacctg ccggcacatt tcagaaccga ttgaaccgcc
    17701 tgctcccgta acaagaataa tcttatcttt taagaatgtt tctatctgtc ccagatcaag
    17761 atttaccacc tcacgcccga gcaaatcatt gatatccacc gtacgcatct gggacacctg
    17821 cagcttgccg gaggcgatat ccgtcagcga cgggacagta ctaaaccgca gcttggtacc
    17881 ctgacagacc tgtatcaccc gtctcatctg ctttcgcgtt gccccgggca ttgcgattgc
    17941 aatctcatca atggcattct tgcggcagat ttcaggcaat tgatcaacat ttcccagtac
    18001 ggttattcca tgaatactca tcccgatttt ggccggatca tcatcaatca gccccaccac
    18061 ctgatagttg acatcctttc gccgcagcat ctcccgcaaa agtgcttcgc ctgcattgcc
    18121 ggcaccaaca atcaaaaaac gccgtacggg tcccttgacc tcggagaaga attcttcatg
    18181 atacagacga accagcattc gaagacctga caggacaata acggttgcaa ataaatcaag
    18241 gaccaagaca ccttcgatag accgtttaat atcgttaagt tcaatatatt ggacttctaa
    18301 aagctgccgg gattcgctgt tgacggctgt ttttttctgc tggcgaacca cttccggtat
    18361 gcgggctaca cggacaagat cataaaggcc tttcagatta gcatattttt gtccatattc
    18421 ggttctttca gccgcaatca ctgaattgct ggaaagttct ttatagaata gaagcttgtt
    18481 ttctaattta tcaatcacgg aaagcggaat aatcttttcg ccccagtaaa catagccgta
    18541 ccagaaaacc agcagaacca gccagcttat aaatgaggct tttgtaatca tcagcagatc
    18601 cgacagcccg acatatctcc accagccgtg aaattgcttc aaacgcgcaa atacaaacag
    18661 tttaaccggc aacactgctg caaacaaaat agggagctga aatacaagcc gccgtcttga
    18721 ggagtagtcc ccgtaggcaa aagccaaggt aaaagcgacc agtaaagccg caaaaaaaca
    18781 gctgatatga gctaaaataa taagcagccg ccggtatcga aatataggtt ttttagtctg
    18841 ctgattatgc tgttgaatct ctgttgcagg catttctggg atccaaagga atgtttttgc
    18901 cgatttgttg cagatgtata taaatccgat tcagttcaac attatccact tttctgcgca
    18961 tcagcgcata gattaccgtt gcggtgctaa aaaaatagct gaccacatac gacagcagca
    19021 atccgacgat taccagcata aaaacataaa tcacaattga tgctgccgat tccgtccagc
    19081 ctactgcgct ggacgaacgt ttgagaaaat ccatgaaatc gggcttgggc caaattcgct
    19141 ccagtttaga gccagctcct gctgtcatca tcccagccag cagcaatccg taggtaaaac
    19201 gcagaatccc gaacaatacc agccggataa taaggtaaaa gaaggtcccg aatattcccg
    19261 acacaagaac ataataaaac atccgtaccg gacaactgag gacatagcag aaggaccggc
    19321 caattgaatc cagcccggtt gttttctcat acgcaatggc aggaaacagc aggagtcctc
    19381 cggctattgt tcccataacc ataaccgtaa tcacaaaacc aaacaccagc agcagcccaa
    19441 acagcagtgc gataatcagc tcccctgcat gcgaaatgga accagccaaa ccaacagcaa
    19501 aaatcacaaa tgccgccaaa ctcaccagtc ccatcggcaa taatggcgcc gagagaaatg
    19561 cacggcaatt ttctgttaca taactaaccg cttccagcaa accgggcttc tcgccctgtg
    19621 caaactccag cgcagcacag cggcatatgg ttcctcccgt aaagacaaaa accagaaaag
    19681 ctgccgtaaa aaaaattaag ctgtacatcg ggtgaaaacg aaaagcccag ccgacagccc
    19741 gaatacagag ccatatattg gtcagggcaa actttacatt cgcaaaaaga ttagcattgt
    19801 ccagattcag caattgaaaa accgcggcat gaaaccggcc ggccgcaaat ttccaaagtg
    19861 tcgaaaaaac accttgccga tatgtctgcg tttggtgttc tttgataaac cgttttgtct
    19921 gggcaggatt agcggcaata tagatgtcca gttccgaccg actcgccaag ccagctgctg
    19981 cagtctccgg acgatagaca attacattct gactgcacat atccattact gcacccagca
    20041 cacacacggc aaggacaccg ctgaaagcaa taataatttt tgccggcccc agcgccagct
    20101 taaaactata cgctagatcg ggcagtgcag attcttccca tatacttttg agtgagtatc
    20161 gttgcgtcaa attacccctt tgtcaaatct atttgcccct cttcacccga atgccagtcc
    20221 tcatttgcag ccggcttttc cgatgggata cggtacttct caccgaacca gccgtccaaa
    20281 tcaacccaac ggcaacgctc actgcaaaag gggaaaaaag gggcgttaat ctgcttcttt
    20341 ccctgcagag tcgtggacgt cgaaggaatt acttttccgc atatcggaca tttgaaatcc
    20401 attgtctttt ttgatgcctg cctatcaaga caactatttc gaatgattct cgtttaacaa
    20461 aatccagatt gttctataac aaattcaaaa gtgtattttt ttctgttcaa aaaactactt
    20521 tagctaataa ttttcatcgt cacttttatt gctgtctaca tcaaaaatgc cagtattggt
    20581 ctgctcttgg atttcggact cttgctcttg atataaattc acctgcttcg atgaggctgc
    20641 tgtctgagaa gcagcggttt cttctgcagc tttacccctt tccatctgtt gtgcgtactc
    20701 aagacaatag cgcgtccatg gaatcccttc taatcgctgc cggggaatgg gatttcctgt
    20761 gccctcgcat attccatagg ttcgattctg gatgcgcttc agtgcggcca gtatctccgc
    20821 tacaagcttg cgttcgctgt ccatcagtcc aagagcaaac tcttgttcat aattatcgct
    20881 gccgatatca gccatatgaa tcggcatact cgacaaatct ccgctggcat ccgaacgaga
    20941 agatttcaat gcgccggact cgatttgata aacatcacct gtaatttcat tgagcttctc
    21001 aacaagtagt gtcttaaaat gctgcaaatc ctgctcactt aaccacgttt tctgtatttc
    21061 caaaagaggt tcagacgagg gatttaccgg cgaagatttg gatttttgcc gagaagtgtt
    21121 cttttctggc ggaccggtct tttttgatgc ttttttcccc gcggaaggta ccctgttgcc
    21181 tttgccggct gcagttttgt gttccttttt tttggtcctt tttatcacca aatcttaccc
    21241 tcttttctgg ataaccagtc gttttagctc ttaacataga attatttatt atatataaaa
    21301 tatcgtcatt tcttcaatca aaaatatgtt tgtttcgccg gaaaaattaa ctccaatata
    21361 agacattcgc agtccaaata ttaatatttc cgtttatctt ttgaataatt tcctaaggga
    21421 aggaaaaatt tttctgcaca tacaagacct tgatgcactt taacagaaaa aaactgctct
    21481 atgatttctg cagtcagcag ttcattaggt tttcctgttg cataacaacc atcttttcca
    21541 accagcagaa cataatcact gtattgacag gccaagttca aatcatgagt aaccatcaga
    21601 attgtcttgc cctgctcaac ctgcatccgc cgcagcaagt caaaaatctg aatctggtgc
    21661 ttcatatcaa gatggctggt tggctcatcc agcaatagta tcggcgtttg ctgtgctaag
    21721 gcccgtgcaa taaaaacgcg ctgccgttcg ccggcactga tttgagcaag attgcgatcg
    21781 gccaattggt atgtgtctgt ttgtttcagt gcagattcag cagcttcccg gtcatgagcc
    21841 gtttcaaaaa gcgaccactt ccgtaaaaac gagcgggcca ttagaacggt ttcaaacacc
    21901 gtaaaatcga acaccggtac aaattcctgc cgaacatagg acattttttg tgcccgctgg
    21961 ctgggtgacc acgattccag cggccggctg tccagcccaa cagcaccctg ccggggcttc
    22021 aattgaccag acagcagcct taagaatgtt gttttccctg ctccgttagg tccgactatt
    22081 gcccaaaaag agtttgcctc aatagacacc gaaaggttct gcaagaccgg ggtattgtca
    22141 tatccaaacc aaatcgattg taactctata aagcccatac atgctttcaa tgcatacttc
    22201 ttgtacagcg gaccagcaga attaaaaaaa acggtcctcc cgccaaagcc gtaatgaccc
    22261 ctaccggcag tatcgccgga gccactgcaa tccgggccgc tgcatctgcc gccgccagaa
    22321 aagcggctcc tgtcagcgca ctgagtggta ttaactgccg atggtccggt ccagctatca
    22381 gccggacagc atggggtaca atcaaaccga caaatccaat aagcccgctg agtgatacag
    22441 ccgcagaggt catcaaagcc gcagctgcaa aagccagaaa atacgtttgt tcaaccggca
    22501 ctcccatgct gcgggcttcg tctggcccaa agctcaaaat attcagccgc tgtcccagaa
    22561 gcattaaaac aataattcca aacccacata taccgccggc agccccaagc acaggaccgt
    22621 actccaattc tgcaaagctg cccatcagcc agaaaagcgt cgtctgcaga tggccggcct
    22681 tcacaatcga aatcaaaaac aaaatccctg ccgataaaaa agcattcaca acaacacccg
    22741 ccagtaaaag ccccgtggtt tgcaggcgac cggtcatccg cccgatcccc caaaccagcc
    22801 aaacggttgc catcgctcca ataaatgatg ctgccatcat tgccgacaag ccgccgaaag
    22861 tccatgtcca gcccaatata acagccagca tgctgcccaa accggcaccg ctggaaatcc
    22921 ccagaatata cggatcggca agaggatttc gcaggatagc ctgaaataca acaccagcac
    22981 aggccagaga tgctccaaca acagaagcca ataaaaccct tggcagccga aaatgcatta
    23041 taatttcata atcaatattc gatgcatttt tctccccctg catcgccttt cgcaacgaaa
    23101 cagactctga gccgatgctg gtacaggcaa tcataaccgc tatcaggagc actcctgcag
    23161 ccagaatccg cagggtcagc tttccgcaag tcaggatttt catcagttct ccctcgggtg
    23221 caaacattca gccacctgtt ccatcccctc cgccagccga ggtcccaagc ggctgaccaa
    23281 atccccatcc agcacataaa ttcgccccgt ctgaactgcc ggcacaccag caaaccggcg
    23341 gtaaaactgt gacgctgtct cctgttgtct tttcagtgat tgggcatcat cagctgtttc
    23401 aattatgaca tccggcttgg ccgccaacac agtttctgcc gcgataggcg gatactgatg
    23461 cggggtatcg ccaatggcat taattcctcc agctgtttcg attaactcgt tcggaaatgc
    23521 tctgattcct gccgctcgca agggttgacg ctgaatcacc cataaaactt ttggccgata
    23581 gggcaatgct tcacagcgag ccgcaatccc cctctgctta tctttcaact gcgttgatat
    23641 ctgctcagcc gcttcctttc gatggaccgc atcgccaatt gttaaaatag cctgatgaag
    23701 ctgttcaatt gtttcgattt ccagcgtcaa tgtccgacag cccattctcc tgagacgttc
    23761 agcaagtgcc gcctgctggg caaaactctc cgtaataacc agcgttggcc ggcaggccag
    23821 aaccgcttca atatcgggct gccagaatgt gccgacagtc cgcttctgca gcgctgccgg
    23881 cggataatta ctccctttgg caacagcgac aatttcttta tccaatccca atgcaaagag
    23941 aatttccgtc aaattcgggg ccatcgcaat gatgcgcaag gatgaatccg gccgcccccc
    24001 tgccggaatt tcttttttcg aacagcccgc ccacacggct gtccacagca ccaccgcaat
    24061 tgaccaacag acagctctta aaaactgatt gcgtaaatgt agaacttcca ttctgtcctt
    24121 aaaaaccagt ccgcttaaac tgcttttcca gctccctcaa agaaaaggtt atcgtagtcg
    24181 gcctgccgtg aggacaccgg ctggcacgca gaatgatatc tctgtctttc agcagagatg
    24241 caatttcctg ctccgaaagg ggctgacccg ctttgatggc tgccttgcaa gctgccatat
    24301 cgaggatttc gtgcagaagc cgctcagggt cgccgtgccc gctttctccg gctagttttt
    24361 ctaaggtttc ccgcacaaag tctgccgggt ctgcgttttc cagaagagct gtaaatgcct
    24421 gtacagccag cgtattggga ccgaacggca ccaattcaat gccaagtttc tcaaatagtt
    24481 ctttttggga ttcgtatgct tccagctggg cgcgggtaac ctcaaaactg tgcggtacca
    24541 agagccgctg cgatggcaga ctgccttccc gcaggcgctt gcacatctgc tcgtacaaaa
    24601 tcctctcatg aagcgcgtgc tgatcaatca ccgcaaaacc atcttcgctt tgaagaagaa
    24661 tatagctgtt gtgtacctgc aggtattctc tggcatgcca tcgctcggcc tgttctgatg
    24721 attcagccgc cggagcttca gtctgtaacg tctgcggccc atctgttaat gaggcggaaa
    24781 aatttttccc agacaaactg tccggcaaaa atgaaaattt tgcttggctg tacgacggct
    24841 gctgcttgaa aaaatcctcc atcgcccgct gaatccgctc tctgcgggca acggttcctg
    24901 atgtcagagg catcgcggac aagcggctgg cttcatcaga cgcacaggga attgttccaa
    24961 cggcgtccag attggtactg agcagtttat cccgcagcac cgccagcact tccgaatgca
    25021 ccagattagg attatcgaac cgcacctcca ccttggtcgg atggacgttc acatcataat
    25081 cttccggcgg catctgcaga aaaataaaga caacaggata cttattcggc tccagcagac
    25141 cgcggtatgc ctcacgaaca gcatgcagaa gaaatttatc gcggataaac cgcccgttta
    25201 aaaacaaata ctggtaattt gcggatgccc ttgcggcggc cggcttggcc agcagcgcct
    25261 gtatctggat gccccgttcc gcccgactgg tttccagcaa atcggcggcc attgcctgtc
    25321 cgaacagaat tgctacccgt tcgcggatgc tttgtccggc cagcagatga taaacctgac
    25381 gtccattatg tatcagcgtc aggtccaaac tgctgtgcgc taaggcgatg cgggtaaaat
    25441 gctcgataat atgagtcatt tcggtattgg gtgtccgcag aaatttccgc cgagcaggga
    25501 ttttataaaa aagactgcgt acctcgatga ccgtcccaac agcgccgctg cagggacgca
    25561 ctggtttttt ctggccgcag tctatttcca caacggcagc ttcaatcgaa tccgctgtcc
    25621 ggctgacagc tgacactttg gccaccgcac cgatgctggc tagggcttcc ccacgaaatc
    25681 ccatcgtccg aatggcagat aaatcttctt gacagcgaac tttgctggtt gcatgcggct
    25741 caaaggccaa tgccaaatct gccgcatcca ttcctgcgcc gtcgtcggta actcgaatca
    25801 aatctcgccc gcctccttcg acttcgacaa cgatttgcga tgcgccagca tcgatgctgt
    25861 tttcgagcag ctctttgacg acactggcag ggcgctctat cacctcgccg gcggcgatca
    25921 tattgatcat cctatcatcc aatacggcga tcttgcccat ttattcggct ccgttcaaaa
    25981 ttcgaggtgc acccgtcgaa ttttgaattt ggtcctatat caacagactt cgtccggtca
    26041 tttctgccgg cttcgggaga cccatcactt ccagaaaggt cggcacaaca tcagccagac
    26101 ggcccggtct catcgctcgg cccttaaact ctttatcgac aacaatcagc ggcacatcgc
    26161 caaccgtatg cgctgtgtgc ggcatattat tttcaaaatc ccacatccgc tcaaaattgc
    26221 cgtggtcggc cagaatgaca gcgctgcccc ccaatgccag tgttttatcc acaattcggc
    26281 ctacgcaggc atccaccgtc ctgcaggcgg tcactgccgc atcaagaaca ccggtgtgcc
    26341 caaccatatc cggattggca aaattgcaga taactgcatc atacttgccg gagtccagcc
    26401 ggcttaatac ctccagacaa acctcgttgg cgctcatttc gggcttgagg tcatatgtgc
    26461 gaaccttcgg ggaaggaatt atctgccggt cttcgccttc aaacggggct tccctaccgc
    26521 cgttgaagaa aaaagttaca tgtgcatact tctcggtttc tgcacagcga aactgcttta
    26581 atcccagcct gctgaaatac tcccctgcta tattgggcag ttcccgaata ggcggaaatg
    26641 ccaccggtgc tttgatggat gcatcgtatt cggtcatgca tacataatgg acttcaggat
    26701 gtacagtccg ctcaaaagca tcaaacggat tatccacaaa tgcccgtgtg atttcacgcg
    26761 ggcggtcgcc ccggaaatta aagaaaatca cactgtcgcc gtcttcaaca agagccaagg
    26821 gcttgccgcc gccgtcaaca atagccgtag gaataatgaa ttcatcggtt tcctctcgtc
    26881 cgtaacttgc ctcaacagct tccgctgcgc tgcgggcttt attaccgatg ccctgtgtta
    26941 gacatttata ggcctgtgct acccgctccc agcgtttatc gcggtccatt gcataaaacc
    27001 gccccattac cgaggctatc tgtccaacac cgatttctgc cattttggat tcaatggctt
    27061 taagataatc ccgcccgcta gtcggcggtg aatcgcggcc gtctgtaaag gcgtgaagat
    27121 acacccgttg aatcccgcgc cgcctggcca gttccagaag tccgtataga tgccccagca
    27181 gggaatgaac accgatatcg ctgcacagcc ccatcaggtg aagcttgctt tttttctgga
    27241 gagcattatc aatagcagca ttcaggatag gatttgtaaa aaattcgcct tcccgtatgg
    27301 cgacagttat gcgtacagaa tcctgataaa cgactcggcc ggctccaatg ttctgatggc
    27361 cgacttcgct gttgcccatt gtcccttcgg gcaatcccac atcctcgcca taagtacgaa
    27421 caagacaatg agggtattga gccatcaatt cggcatcaac cggcggcttg gccaccttga
    27481 ctgcattgaa tgcatcttct tccggccgcg gattatatcc ccagccgtct cgaataatca
    27541 ttacacaggg ccgctttctg agcgttatct tatgttctga cattgtttta gggcctttct
    27601 cctgattcca gattttgacg gttctcataa gttggttcag tgtaagaaaa tatgccggcg
    27661 gtgtcaattg ctattcaggc aaattaaaaa caggcaaaga gcagacggca caaaaatctg
    27721 cagcaatttc ggacagaaca gagccggaag gtttttattg tgaaatccct ttgacagagc
    27781 atttaatttc gctataatcg cgtctaaata taccttaaat aaggctccga cgggtatggt
    27841 ttggggcgaa aaacacataa gaaaggattg tactatatgc tttcgaaaaa atgcatctta
    27901 atgcagttta tggcagctgt atcattcgtt cttgtgttcg catctggatg ccaatccccc
    27961 cagacagcag tttcagatcc aaatgcggta atttctgccg ctcaaaacca gccggcaccc
    28021 caagcaagca ttgatcccaa ggagcaagcc gtaagcctgt atgtcgatgc aatgatgctc
    28081 aacgacctga acgaacgcga ggaggctatt cgcaagatca atcaggcatt ggagttagat
    28141 ccccatttct caatggctta ttcgctaaag ggagatattt tgcagggaat ggagcagtac
    28201 gaacagagtg ccgatgccta tgagcaggcg accctatacg atccgtggtc attcaaggac
    28261 tttttcaatc tcggcaaggt gtgccaaatc ataaagcaat gggcacgagc cgccaaagcc
    28321 tatgtatccg cctgcacact cgatcctcag cactattcgg ctcatctggg ggctgcacaa
    28381 tgcttctatg agctgaagga atatgaaaac tctctgattt acgcccaaaa agccaaagaa
    28441 ctggacccag cccaagccgc tcctgaacga ctgcttggcg acttgtatgc gtcccagaaa
    28501 gaccatctgc aggcgataaa tgcctaccgc cgcgttcttg aaatggaagg caacaagccc
    28561 gatgtaatga tttcgctggc tcgggcttat ttgagatcag gccggtacaa ttcagccaaa
    28621 gaagttttaa cagatgtcat tgcgatagaa ccgcagaaca gtttggctta tcaatatctc
    28681 ggctttgccc agctcaagct caaggaaaca gaacaggcag tcgaaagcta tcaaaaagcg
    28741 gttaatgttg gccaagacga ctggatggcc cataaaggac ttggggtggc gtatatgctg
    28801 ctgtcaatga aaaaacagaa cgatgaaaaa atccaggcgc tggcaattga acagtggacc
    28861 atttcgcttc aaatcaagcc ggaccagccc aaacttcagc agcttataga gcgatattcc
    28921 agataattcc tgccgatttt ttgaatgaag gataaaaaga cacactatgc gcagccggac
    28981 aatacggttt tactgcatac ggcggcagtt tcaatgacgc agaaaatcct gctgttttgg
    29041 ccggctgctg cgggaggagc cgcactggat ttatggtcca aatgggccgt tttccgctgg
    29101 ctcagtactg aggaaggata tcgctattcg attattgacg gctttctgca gtttgtgctg
    29161 agggaaaatg aaggagctgc attcagtctc tttcggggat ggacagcatt tttggttatt
    29221 atctctgccg gggcattagt tttcgtaata gggctttttt tctttgcgaa atttaccagc
    29281 aaactcgtac tgttttcgat gggatgcata acagccggca ttatcggcaa cttgtacgac
    29341 cgcatattca atgacggccg ggtgcgagat tttatcgatg tctatgtcgg accctatcac
    29401 tggccgactt ttaacgctgc cgattcactg ctgtgcatcg gtgtgggatt gctgattatt
    29461 gccaatttca catcgccagc tgctcaaaga catgatcttc cacaaacaga ggcacattaa
    29521 gccctgcatg cagggcgatt gcatctgaag gccggctgtc cactgccacg acagaattgc
    29581 cctgccggat ataaattaca gcgaagaagg tgtgctcccg caggtcgtta atgacaactt
    29641 taaggatagt tcctcccatc tgctcaatga cattagccag caggtcatgg gtcataggtc
    29701 ggggcagttc aattcctttg agccggcggt ctatcgctaa aatttccggc agcccgataa
    29761 ctatggggaa tgccctttgc tggccgttca gctcccgcag cacaataatc tgctggtcag
    29821 cctcctcatt gatgataatc tgggctaatt cgacaggaat ttccataacc ggtatccatt
    29881 atttgcataa tatcatttat aattaaagca ttatacaaaa agtctttatt caattcatct
    29941 tccaaaaaat aatttcttta atttgagtcg aaatcgtatt tatttggatt ttacggtttc
    30001 tttaagctga aaataggatt ggggagtaaa ttataaaaac tgttagattt aaagaacaat
    30061 ctgattttct tctttttatt gtcctatatg atatttgcca attatgttta ctggacaaat
    30121 agttatgtat ttgcaaaact tatttaatag gattgctgta ttttttcagc cgacaattgt
    30181 caacagttct tatggattat ggaatcaggg gcagacagtt gatatacttg cctagtagct
    30241 gcagtatatt tcccgcagtc aaataaaaaa ggcctcgcag gtggaacccg ccgaggcctt
    30301 tttatatact ttttaggagc cggattacgg catcggcaca aacagtcggc tgttgagcca
    30361 ttcggacgcc agcagtccca aatcggccag attgacgaca cagtcgttgt ttaggtcgta
    30421 ggtctgggac aggcagacat tcgtcccagc aagatccgca tatttctggg caatggtttc
    30481 cgcatccagt gcatagttgt aaatttcaat ttcatcgtag atgccggcta ccacactgcc
    30541 aagttcaaca ttgagacctt cagttagatt tcctctaacc cggccggcaa tcgacacagg
    30601 ggaaccggtt gagccgatag gcccgctgac gggatcactt tctcggacaa catccccgct
    30661 atcgctggaa tacagccggc tgacaacacc gtcgatatag acctttttga cagcaccgtc
    30721 ataagttccg acaacatagt gccagatgcc gtcatagatg gtgcggttgg cgggtgtgcc
    30781 gtcatcgtta tctgtatcat tgggggttcg ggtggtaaaa cacgggcggt cattggtaaa
    30841 tccgctttga cgcagctgcc agccttgacc atcttcgccg tttcgggcca ccagcggaga
    30901 ccaactctgg cgatcggtag ttttaaccca gcaggcaacg gtcatttcat agttacagat
    30961 atcaaaatat gcgttctgtt caggatccat aaagagcatg tccactccgt cggtaaattc
    31021 cacagcatta ccgagaatgc cagccggtac aaaattcggg tcaccgacaa ctattgtgtg
    31081 cggactgccg gcaactgaac tgccaaatac attgttttca aacggccaat atgcaatccg
    31141 tcgtttgatg cccagcggcg ctttgatgga ttctgccgga atgcctgatt cattattgac
    31201 aacgcagaaa taataacctt catcatcgag gccggcatcg gcaatcgcca acgtattggt
    31261 tccggtaccg gaaatattgc cgccgtctgt caggagaata tcttcaccgc cggcaacacg
    31321 ataccattgg tacactgtcg gggattgcga gtcgactgtc acagaaaaga ctgcagtgcc
    31381 gccggcatcg ataatctgat gccccggatg ggaaagaatg ataggttttg agaattctgt
    31441 atagaaaatc cattcaatac cctgccgcgc agtattgtca ttcattaccg aatcaacacg
    31501 ccatcggtag gtgctgtttt tctgcagttc ctgagccggc acatatgcag ctcgcaaggt
    31561 ctgtggatcc cacacagtaa catcaatcgg cgaggcggca ttcaagttcg ggtctgtgca
    31621 gctgtaatag agcttgaagg aaaccaagtt gggatcggcc accatcactt gcggatttac
    31681 cgaactgcgt atctggggaa tggcccatga cagttccagt ccagcaaccg gaacatattc
    31741 ctgcttataa gacggcatcg gagcatgggc agttttgagc cagtcctcgt ggattcgcag
    31801 attgtcgaac cattggtcac tgtcgttgcc gccctcggta taaacctgga ttccggtaat
    31861 ggccgttcca gtgaatgttc ctacccatct gtcaataatc ggaccgacat tggaattgcc
    31921 gtcatcagcg ttgttgcccc atcctgcagg attatcggga attgaacctt ctgagacggt
    31981 catctcatac gaattgggcc ccgttaacgt aaagacgacc tgaaagatgt cgtaccggtc
    32041 ataatatttg gcaatatccg tatatgcacc tgtaccggta tcccatcgcg tgtaaacaat
    32101 ctttccgccc aagaagacaa gagcgacctc ggggctggag ccattgagca gacgaatgcc
    32161 gatcgatgtt ccgttatcga tgctgatgtt atgtgcccag tcgatgctga cggactggcc
    32221 cacagtcagc gggccgccgg caaaggtatg ggttgcatac gttccggccc cgccgtcgag
    32281 atcccaaatg gtccacatat atgggttgcc gtcttgcata tcctttagaa agctgcgtga
    32341 agcacctccg tctccggtaa ccgcccagac atctactgtg ccgttgtttg catcgggcga
    32401 agagcccgga ctgccagatg ccgtgtagga cacaaggtct gcggcatttg caaaggctgc
    32461 aaacaggcat acaaccactc ctgtcatcca tactttcctg caatttctca tctttttacc
    32521 ctttccgaaa agttttattt cttaacctgc caacggttag taaacttgct gattgttttg
    32581 ttaattttga aaaagagcag gcaaaaatat attaagcgtt tgacattata cagatcctga
    32641 tgcggggaat tttaataata ctcgtaccct ctctttgtac tattgcttca ggccacaggc
    32701 agttgaaatt gatatctcca ccttaaaaac ttcttcattt tcagccctgt atgattccca
    32761 tctctccgat acctgccgac cgaatactcc ggaacaagca tgcttttttt attatactct
    32821 attttccgtt taaatcgcta tcaaaataaa aacttatcta attttattga ttcctgttta
    32881 tcgctaaaca agattttttt gctttacaga gcgcaggcat tagggataat aaagggccaa
    32941 tattaatctg ttttttaagg agaaaaaaaa tgaaaatgta caaagtttta ttggtttgtg
    33001 tgttactcat tcctttgcag ggattttcaa aaaccacaat tcaagatgcc gatggcgtta
    33061 atcgtcttac gatttatgcc gatcaggaaa aaggcactat cgcaccggag atttacgggc
    33121 atttttccga acatctgggc cggtgcattt atgatgggat ttgggtcggg ccgaactcta
    33181 atattcccaa catacgggga atccgcaagg atgttctgga ggccctgaaa aatcttaata
    33241 ttcctgttct tcgctggccg ggcggatgct ttgccgatga atatcactgg aaggatggta
    33301 ttggaccgcg tcagcagcgg ccccggacag tcaatactca ctggggaatg gtaacggaag
    33361 acaactcatt cggcacacat gaatttctgg acttgtgcga actgctgggg tgcaaggcct
    33421 atattgcagg caatgtcggc tcaggtaccc ctcaggagat gatggaatgg gtggaataca
    33481 tgacctttga cggcgacagc accatggcta atctgcgccg gcaaaacgga cgcgataaac
    33541 cctggaaaat cgattatttc ggcgttggga atgaaaactg gggctgcggg ggaaatatga
    33601 cgccggaata ttacgccgat gtttacaaac gctaccaaac ctttgtaaaa aattaccctg
    33661 gcagccggat aaccaagatt gcctgcggtc ctaacggcaa cgacacacgc tggatggaag
    33721 ttgtgctggg aagggccggc cagcaaattc aggccatcag cgtccattat tatgtgatga
    33781 gcggttcgtg gggcaacaaa ggcagcgcta cgcaatttga tgaaaatgag tggttttccc
    33841 taatggataa aaccgtgggc gtcagaaagc tgctgcaaga ccaaatctca gttatggacc
    33901 ggttcgatcc acgcaagcgg attgctttgt acgtagatga gtggggaaca tggtgggacc
    33961 aggaacccgg ttcccagccc ggttttctgt atcaacaaaa tacgctgcgg gatgcggttt
    34021 cggcagggat ttatctcaat gagttcaata accactgtga ccgcgttaag atggccaata
    34081 tcgcccagac agtcaatgtg ctgcaggcaa tgattctgac cgaagggcca aaaatgctgc
    34141 tgacgcccac ctatcatgtg tttgagatgt ataaagtgca tcaaggggcc agactgctgg
    34201 aaaatgaatt ggtttgcaat gcctatgcca aagggggcac aaaattggct gcactgaatg
    34261 tctcggcatc caagaaagac ggcgtaattt cggtttccat ctgcaatctg gatccgaccg
    34321 ctccggcaga gctggagtgc cggctggacg ggatgcaggc acagaaggca tcaggacggg
    34381 tgctgacagc agaagcgatg aatgctcaca acacctttga caagcccaat gcaatccagc
    34441 caacggaatt aaaaggtatc gagattaaag acggccttgt ccgggctacg ctgcctgcaa
    34501 aatcggttgc gatcctaatt atacaataat ttgaatacaa acatgccaag agccggttag
    34561 cagccggctc ttttttttgg caagatattt cagctagtct gcggggcggg attgttcaat
    34621 tttcatattt atgaggctgg cagcgtaggc ggcaccgaag ccgttgtcga tattgacaac
    34681 cgtcacccct gcagcgcaac tattgagcat cgccaacagg gctgcaagtc cttcaaaact
    34741 ggctccgtag ccgacactgg tgggcaccgc aatcacagga caatcgacca gcccccctac
    34801 aacactggct aaagcccctt ccattcccgc tactacgatt acaacattgg cctgccgcag
    34861 gacttccaaa tgtcccaaca gccgatgcaa acctgctact cctacatcgc agaccatctg
    34921 cacattctgc cccagcatct gtgctgttac ccgggcttct tcagctgcgg gcatatcggc
    34981 tgtgccggct gttacgatga caattctgcc gacagaggct tcaatcggcc gctgctgcag
    35041 gataataatg cgggcggcct gttcatatcg gagagcatcc aaatttgcgg tgcttttgac
    35101 tctttcaaac acttctgcag atgcccgcgt agccagaata gtatggccgg ttggcgccag
    35161 ccgctgaaag atttcggcaa tctgctctac cgttttgccg gggcagtaaa tcacttccgg
    35221 aaagccgcat cgcagcgagc gatgatgatc cactttggca aatccgatat cctcaaacgg
    35281 cagatgccgc agacgttcga cggcttcaca cacagccatg cggccttcgc gcacatccgt
    35341 cagcagagtc tcaatttgct tccgattcat tggatttttt gtactgctac tgtactgtta
    35401 attggagcac tgtcatggaa tagggcttaa acaaatattc aaacgccgga ccctgcacat
    35461 tcacaggttc gttaaccggc aagacctttg ccggctggtc gattgtattt tcatcctgcg
    35521 gaagaccagt tagaattacc gactgtgctg agctgacaga ggtgatgcct ttaaggttaa
    35581 tgtctgcagc aacggatttg ctggatggat taaccgcttt caaaataatg gatttgccgc
    35641 tgcggacagc cgaaacaaac aatgtctgcg tctgtctgca ttcggccggc aaaagagcat
    35701 caccgcggta attgctgaac agcttttgga cataatagct gggtgttcca tatacggaca
    35761 ggttgtcaaa ccaaatcaga ttgggcttcc actgccaggc atctacgtga gctgtcagcg
    35821 gagcataggc tgccatccta accaaatcgg cattgcgttc caaaccggtc ataaaagctg
    35881 cttccgccag cgcaccggcc agattgtttc ggttattcgg actgccgaca ttctggctta
    35941 tggcagcata ttcaccgata aatatgcgag gaccgctgcg tttataggca tcgtaacggg
    36001 cggcattctg aataaaccaa tccgggggtc gataaaaatg ttcatccacg atatcggcat
    36061 tgagccgacg gagttcctgc cataagtatt caacttcttt ggggccattg gggaaaatcg
    36121 ccggatcgga accggttgct ccaatcagct taatatcggg atatttttct ttgatggcct
    36181 ttacaaaaac cttataccgt tcgatatact gtggacccca ttgttcattg ccgataccca
    36241 aaaatttcat attaaagggc ttgggatgtc ccatatccgc tcgttttcgt ccccacgggc
    36301 tgtcagccgg tccattggca aattcaataa gatccaaagc gtcctgaaca tagggtccca
    36361 gtttatcaac agctgccgtc tcaccggtgt tgaactggca ggccatcccg cagttaataa
    36421 taggcattgg ttctgcacct attttttcac agagaagaaa gtactcgaag aaccctaaac
    36481 cgaatgactg ataataatcc ggcgtgaggc ggtgtttaaa ttcggtattc cagcggttga
    36541 tgattgtttt ccgctggttt atatcgccta tggtattctt ccattgatac cggttgctca
    36601 atgtaactcc ttcaacaatg cagccgccgg ggaaacgcag gaaaccgggc tttaaatccg
    36661 ccagcattgc aacaatatct tcacgaagac caggaatagg gcggtcggcc atctgagctg
    36721 aatctgcagg aaataaggag atcatatcca aatcaacagt cccgaaggaa tccataaata
    36781 tctctaagtg ggcttttggt tctgtggcca tggcctccat tttgcaggtg tagtttttcc
    36841 agtcgccggt aatgttctcg attctccctt ccatcagttt ttcagttccg ctgacgacct
    36901 cgatacgcag actcacggcc ggcgaaccaa tattgcgggc aaaaaccgag aaccaatagg
    36961 acctgccctg ccggatgccc atcccgcgaa atccttcgtt ggcaattcca aagggctgac
    37021 cccgcgatgc gctcattttg agataatgag gatttttttc atccacaggc tctttaacga
    37081 ccacagcagt ctgtccgtcc gagtcagcgc tgagaataag agaccagccc atcattggat
    37141 tgtcgaattc aaacgagcgg ttcttaacca attccgcata gagaccgccg tcggctccga
    37201 aatttatatc ctcaaaaaaa atgccccaca ttgtcgaggc aattggagta ccaggctgat
    37261 cggcggaaat ggtgattgtt gattgggctg atgcgattgc tgccgtactc atcaacaccg
    37321 ctgcatggaa gatttgattt agtttcatct tatactcctg gacaattgga ttccgatttt
    37381 taagctgttg cgctgggctc cagatccagc ccagcaaact gctttgcctg atacttatag
    37441 tatgccagcg aggccaccat cactgcatta tcggtacaca gctgcttggg agcaatcagc
    37501 aaagtcagat gctccctgct gcacatcgct tccattgcct gccgcaatgc actgttgcac
    37561 gccacgccgc cgcccagcag gactgtttta gccccgattt ggcgggcggc acggcgggtt
    37621 tttctgacaa gcacctcaac aacggcagcc tgaaacgaag ccgcaatatc ggctgtctcc
    37681 tgacggctca tagacgctgc ccggttctgc cccttcatat cctgcccccg acaatgataa
    37741 agcaccgctg ttttcagccc gctgaatgaa aagttgagat tgtctttctg catcagcgaa
    37801 cggggaaacc ggatggcttt tggatttcct tctttggcaa tcttttctat actgggaccg
    37861 ccgggatacg gcaatccaag gatggacgcc accttgtcga atgcctcgcc ggccgcgtcg
    37921 tcaatcgtcc gtcccagcag ccgcaggtcc agcgccgatt cagcatcgta cagattggta
    37981 tgcccgccgg aaacgatcag ggcgacagcg ggaagttcta tggattcctg ctccagtata
    38041 gccgattgca ggtgggcgtg gacgtggttg acggcaatca ggggcttgcc ccaggcgaac
    38101 gccaacgtct tggctgcggt aacgcccacc atcagcgcaa tagccaaacc cggctgactg
    38161 gccacagcaa cagcgtcaat ctgggatggt tcaatgcaag cctgctggat tgctttttca
    38221 ataaccggca gaatattttc cacatgggcg cgtgaggcaa tctccggcac tacaccgccg
    38281 tactgctcgt gcagtgccgt ctgcgaggcc acaacagacg atttaatctg ccggccattg
    38341 gccacaaccg ccgcggccgt ctcatcgcag cttgtctcta tccccagaat atgaatgccc
    38401 tgctcggcca aatcggtacc tttcaattta tctgcctata gattagcccc acagccgaaa
    38461 gatgtcaata aatttgaaaa cagcaggtat ttgtttgatt ttgtgccgct ttcgggctaa
    38521 aatacccatc agacttatca agaaacagac gcatgacaac acgggaaagc aacaagaacg
    38581 tcttctttga gctaattcaa acagaagaag gccttgcaaa actgatcgga ctgatcaaat
    38641 gcgtccatac tgtagcattg gataccgagg ccgacagttt tcatcactat cggcccaagg
    38701 tttgcctgat acaacttagc tttaacggat atacggcaat tgttgatccg ctggcggggg
    38761 tgtcattaaa gccgtttctt gaggagctgg cagtaaaaga gctggtgatt cacgatgccg
    38821 gctacgacct gcggatgctt aagggcgatt tcaattttgt gcctcaaaac ggcattttcg
    38881 ataccatgct ggctgcagca ctgacaggaa tggcaaatgt tgggctgtcc agcgtgcttc
    38941 aagaagtact gcagatacag atagctaagc ataatcagaa ggcagactgg tcgaggcgtc
    39001 ccctgccgga ctatttgctc cgctatgcgg cggaagatac ctgctattta atgcaaatcc
    39061 gagacaatct cgagtcacgt cttaacgagc ttggccgtct ggattggcat cgggaatcct
    39121 gccggcaggc tgttgggagt gctatcgccg caaaggaaac tccagatata gataacctgt
    39181 ggcgaatcaa gggaagcggt attctgacag accgtcaaat ggcttttctc cgcgagatat
    39241 ggcattggcg gcacagcatt gccaaacgta ccaatgtagc gccgtttatg atttgccgca
    39301 atgaggatgt ggttaaatta gcgatttggg ccagtcacca ccgaaaacca gtcgaaccag
    39361 atgcccaact gccgatacga tgcaaggcat cataccgaaa tgcactgctg acagcacttc
    39421 aaaaagcgca ttgcctcccg aaagaccagt ggcccagtct gccgccgtcc gacccgtcaa
    39481 aacggctgcc ggattctgtt cgcaagattg tgaatatctt aaaaaccgag tgcgaaacaa
    39541 tcgctaagaa aaacggactg cctttgcagt ggatagcttc ccgggcggcc ctgacggaaa
    39601 ttgttctcaa gcaggcaaca agtctcgaac aaattcagaa agaacagatt cttttaaatt
    39661 ggcaggccaa gcttatactg ccggcaattt ccaaggtgct tgcagagggc gggcaaacag
    39721 atcatcgctt gtccgaatga cggggccatt tattcttcag aagcatccat ccctgcagag
    39781 tgatactttt gcatattcgg ggcagtgaaa atcgtaacgg ggtttggaca gcaggcacaa
    39841 aatcagggca tatcggccca tcaaaggtat cacctgtaat ttcaagagta ctgtctaaag
    39901 tatatcccat ttgttcagca gtctgcagga gcgggagatc ttttggatta aagcccacca
    39961 gctggcagca gattcgttca caagcagcag gattgcggct ggcaatcaaa tatcctatct
    40021 gacgcggcga accattaatc ggcccttgcc cctccatgcc gacaataccg tcaatcagat
    40081 tcaaaaccgg attgagacgc tggaatatac caataataag ccggcaaaaa gactctaccc
    40141 tgtcaccgcg ggcaaagtgc caccatgcct tttccttgcc gcccagccca gcaacgcagc
    40201 cgaacatatt cttaaatgca aatgtggctg tcagctgctg atgggtcttc aatttaggga
    40261 gattgataat ggcatccgct tccaaagctg ttcggctgat tcggacaaca gaatcctcaa
    40321 tttgaatggg aaccggatta tttaactgga caatttcgac ccctaaatgt tttaaacgct
    40381 tatccacacc cagcgctttc aagcaggccc agacatttcc ccaagcaggg gaatctccta
    40441 caagcggttt gccgccggca tccttcacgc atcgggctgc cgcataaatt acttccggat
    40501 gcgtttgtgc ggggatttca gggctatagg ggacaattaa attgggctta atcaaaaccc
    40561 gctggtttct gccgaaaaat gcatctacac cccccagcag ctcaaactgc ctgcatacgg
    40621 cattataaac ttgttgagca tcataagact gacaccgctg gagggcaaca agggatgtct
    40681 gattcatttt agagtcctgc tttgtcgcaa agaatcacaa aaaactgcat ctgcggcttt
    40741 cgtccggcaa ggaggaaaag gattttgcct aaggtgtttt gctcaggcat tttcccattg
    40801 ggccctaaga gaagaaatcc gccttcttca agacgagcca gaacacgtcc tgcttcaaga
    40861 ggcttaaaat ctatcggctg cttgcccatc cgcagccgaa ggtcttcggc gcctctctgc
    40921 caataagacg gaacaaaggt cagctgaaca tgcttttcta atctgggatc ggggcgggcg
    40981 agcatcgtcc atccgatgaa cgtactgggg ggcagtacaa tttcgttgat gcgtccttgg
    41041 aatgggcagt acagaaaggt cagcccctgc tgctgaaaag tacttgataa aatttgaatc
    41101 tcttctggcg gtatcagcag tttgctttgg ctgatgcgga cggctcctat ttcataaagc
    41161 cgctgggcaa tgcgcgatgc ctcctgactt gggcccgatg caatcgccag tccgttgaga
    41221 gcaaatacgt ccctgtttgc tgtacgaata gcctgttgcg acagcatgtc atatacggtt
    41281 ttcagctcat cagcagaggc tgagtcaagc acataggtta aaacgcgaag ttcaattagg
    41341 tattcggcat tcggcttgaa gtttgcgctg tctttcaaat cagagagttt taacccttcc
    41401 acaggtaccg gcgccggcat actctgggga gacacagcgc agccccaaag cagtccgact
    41461 ccagtcatta aacacacagc caataatctc atgcattagg attttacttt tcttaaagca
    41521 gatgtcaatg cacccccgca aaagccggct tcagacacgc agtttcggta aatcgcagaa
    41581 ttctaatgca agcgtttttt ctgtcgactc ctgagaggtt cctacatcag aacgcacaac
    41641 acgggcatag ctggaaaagc tgcctccaaa atcaagcaga ccttccgtgg gaggaaccgt
    41701 catttctaca ctcagcagct ggccatcgga aatatcaccg gccttcattt ctaccagcat
    41761 cccgccgggg ctgacattaa tcgtgcggcc tgaataaata cgaccatcag aaatgcctac
    41821 tttttggcaa aatattgata acttcaaagg aaacctttta tgtctccttc tttcttcttc
    41881 cattttttct gcctccttgc aaaaatacgg atacagaaaa gcattctcgt aataatgtgc
    41941 aaaatacatt ccagtctttc cttaaaacga agagtcaaac ttggtgcacg aaagtcctgt
    42001 ttgtaaaacg atttaaatct actttagcaa caatactcac tagatttctt tatcggcatt
    42061 tcctgttttt gttgataata ataaacgctt tataatttaa ataatttaat acaaaacatt
    42121 ctatgtcatt ttggtctttt aattttttga aacatattct aagattttta ggatgagctg
    42181 tatattgttc ttttttttca acatatgctt gacaaaataa atcgggttat gttcaatgtt
    42241 tactgaaaag aacattgtgt tgcggttttg tctataaaga acatcaaatt tgaaaagcac
    42301 aaatgtctat gcataatcat tcacttcttc aatttgatgc attatgtaaa atgatagccg
    42361 agccattgca tcttggactt atttcttttg accccctgct gaacattatt gactatacac
    42421 cgtctgtcgc caaattcgtt tatttggagc cgaccgtcga tttagcttta ttacgcggca
    42481 ccgatacaaa tatctggaaa gactggaaat cgcttctctt atcctccctt cacacaggcc
    42541 aaaaggcaga tttcggaact gtcaagtata tgcacgaggg acaagttcgt ctgctgaatc
    42601 tctcctgcct ccccgtgcgc aattccgatg ccgcagatct tttgggggga gttttggcat
    42661 ttcatgatac aacagaaaaa agcgatattg agcatggatt agctcaggca gaacggctga
    42721 ttgctattgg aaaagtaaca ggaaaagttg cccacgaatt gaataatccg ctggatggaa
    42781 ttttacgata cgtgaatctg agcatgcgga ttatagagca gggccagccc cagaaggcca
    42841 tggagtatct tcagcactgc cgaacggggc tgcagcgaat gacacaggtc atcaccgaat
    42901 tgcttgaatt ttcccgaagc acacatctgg catttgaaac aagtccagta taagcttttg
    42961 gaagatgctt tgcgcgcaat ggaggggctg cttcggaatg ttgaggtgaa gatacagagg
    43021 gaatatacag cccccctgcc gcatctgaaa agtgattcgt tattccaggt tttctgcaat
    43081 ctgataaaaa atgcagccga tgctatggag ggccgagggc aattaaccat cacaatccgt
    43141 caaacatcca gtgagtggca aatcgagttt cgtgatacgg gtccgggcat agatcccaaa
    43201 catatggatg atattttcaa gcccttcttt accaccaaac cacagggcaa gggcacgggt
    43261 ttgggacttg ccatctgtaa agacattctg gcaaaacttg acggccggat aactgcccaa
    43321 aatgctcccc aaggcggcgg gctgtttact gtatatcttc cgcttcacaa tccaaacagg
    43381 agctgattat gccaaaagct tcaatactta ttgccgacga cgatgccatc attaccaatt
    43441 cactctgtga atttctgaca atggagggat atcaaactgc aggtgtcgga actgtggcac
    43501 aggcacggca gaaactgaaa gagcagcctt ttgcactggt tattacggat gtgaatctgc
    43561 cggatgggga tggtttcgac attttggata ttgttcgggc caattaccct caaactgtgg
    43621 ttcttttaat taccggctat ggtacgattg aaagcgctgt tgaggccata aaaaagggag
    43681 cctatgatta tctgactaag ccgattgtgg atgatgacct gctgctggcg gttgagcggg
    43741 caattcgtca gcagtcttta atgagtgaaa accagcagct tcgccagcag attgaaaaca
    43801 aatacagatt gaataatatc atcagccaga attataaaat gtcgcgaatc ttcgaactca
    43861 tagaagctgt cgccgacagt aaaacgacca ttttgatgac aggtgcttcc ggcaccggca
    43921 aatcgctgct ggccaaagcc attcatttcc gctcactccg ccgcgaccag ccgtttgttg
    43981 aagtaagctg cggggctcta cctgaaacgc tgctggagag cgaactgttc gggcatgtaa
    44041 aaggggcgtt taccggcgct atcagcgaca aggaaggaaa atttttggct gccaacggcg
    44101 gtacgatttt tttggatgaa atttcctcgg cttcgcccgc gatgcaggta aaactgcttc
    44161 gcgtgcttca agagcgtcaa ttcgagccgg tcggcagcaa ccgcactata acggtagaca
    44221 cacgggttat tttggcctct aacagggatt tggctgagga agtgaaacaa ggccgatttc
    44281 gagaagattt gtattaccgg attaatgttg tcgccatcaa cctcccgccg ctggccgaac
    44341 ggataggcga tgtgccgctg ctggctgacc actttctcaa acacttctgc cttgttcaca
    44401 accgccgcaa gtcaggtctg actgaccaag cccttcattg cctgcagagt tatcactggc
    44461 cgggaaatgt gcgggaactg gagaacgtta tagaacgggc tgtcctatta agcaaaggac
    44521 cgctgattga tgttgaagac ctgccgcaaa atgttgtgca aaatagtcat cgtttaggga
    44581 attacacgca tcagagctta aaagaagctc tcgaaggacc tgagcgtgct attattcgag
    44641 ctgcgctgga agccaacaac tggaatcggc aggaaacagc taaagccctt gatatcaacc
    44701 gtacgacact atacaaaaaa atgaaacgct acggtctcga agaagaagcg cagcgcatag
    44761 gattgtaata gtcaccgctg tcccacatta cagctatcaa taatgtggat acaagttgac
    44821 aatattaaca agagcgggtc tgtaagccga gttctgttcc ccaaaagggt gacggtcatt
    44881 acactggacc ggccgttgcc ggccggctct gcccaaaagt ttccttttcg gcaagcaacc
    44941 tacccgctgt gaatgttcgg gccaaacaca cagcttcttg gtcttgcagg cggcggggtt
    45001 tgccgtgccg ctgccgttac cggcagcgcg gtgcgctctt accgcaccgt ttcacccttg
    45061 cctgtgccat gaaaggccat cggcggtcta ttctctgctg cactttcccg cgggtcgccc
    45121 cgggcggctg ttagccgtcg ccgtgcccta tcctgctcgg actttcctcc ggatgtcttt
    45181 caatccggcg accgtccgac ccactcttat tttttattat acccattttg gaactttgtt
    45241 tacaatccct tttgcaacat atattgcagc ttcttctata tctcatccca aacctgcaat
    45301 gatatacaat gatatatttt gctttattaa gaacattaga tatattaaca ggggaaaata
    45361 gtatgaatct ttattaaggt gtgtcttatg tctcaaaaaa agattcttct ggtacagatt
    45421 gtatgtttat ttctggttct tgatatttct tgtgtgtgtt tgtctgctga aatcaatccc
    45481 tatggaatct gtgctcatgt ttcccgcccc agtgatcaca atctggcaga acaagaattt
    45541 gaactgatgc gccaagcggg tatttattgg gtacggactg attttgattg gactaccgta
    45601 cagcagcaac aggatgggcc gtgggattac tctgtgctgg atgcaacagt gaataaggcc
    45661 gagcaggcgg gcatacaaat cctgccgatt ttagcttatg acacgccgtg ggcgaggccg
    45721 gcctataaac atttagataa atggatagag tatgttcgca acacagtgag ccgctaccac
    45781 cggcagctcc gttattggga gatctggaac gagccggatt tggagcagtt ttggaaagat
    45841 aagcccaatc ctgcagacta tacttccctg ctgaaagctg catatcagga aatcaaaaaa
    45901 atcgatccgg aattaaaggt tctgctgggg ggattgtcag gcattcctta tgaatatatt
    45961 gaaggcatct atcgggccgg cgggaaagat ttttttgaca ttatggcagt tcatccctat
    46021 cgatatccag atgcgcctga actaaactcc cttagaaatg atttggataa actgcgtcaa
    46081 ttgatgagcc ggtttgggga tgcagataag cctatctgga taacagaaat cggctggccg
    46141 acacatcaaa atggagttcc tcttctggat gatattatcc ggtgcgcgct gaattatctg
    46201 gaacccagtc gtaaaaactg gacattggca gtattaagta ttccggaata tccgccgcga
    46261 tattcgtatt cggatgaacg cttacaggga atgctgccgg gaggcgggcg tattgttcat
    46321 ttgaacattg aaaccataaa ctttttaaat ccccagatac ataacgccct tctgatgccg
    46381 cccgatgagg cattccctgc cgactgcttt gatattattg aagactatgt ccgcaaaggc
    46441 ggtattatac tctttacaca aggtgtgccc ctttattaca ccatccgcca agacagtcaa
    46501 ggaaattgga ctcggaataa tgcagactca tcctatcgga aacgacttca tatcggttgg
    46561 gaagcatggt ggacccgccc cggcgtcccg cggcagattt caacattatt agtaccaata
    46621 gatttcagaa cccaagtaca cctgcccggc acccaaataa ccgctgaacg atttctaact
    46681 gattctgagc tgaagacagg ggataaattt atacctttgc tggaggctgc cgagggtgac
    46741 tataaaggca gtgcagcggc agtttttgat ttagatagcg acttaaaagg cgctgttatt
    46801 gtttcggctt tgattcaaga ccttcgcggc atttcggaag agcagcaagc cgctattctg
    46861 ccgcgtgcac tgctggccgc attgcacagc ggggcagaat gcgtattctg gtacaatttc
    46921 agggcttttg aaaacaatcc ctattataat gaagatcatt tcggcatcat tcacaaagat
    46981 ttgcgtccaa agcctgcgta ttgggggata caatccctga ctcgtgctcg accggccggc
    47041 tcaaaaccgc tgatggagaa ttggcaaact gaagacttat tttatcccgg ctggaaacga
    47101 cctgatgggc agaacggcta tgccgtatgg actacgggaa aacctcgtta tgcgggattt
    47161 cttgccgagg gaaagatttc cggcatgttc gacaattcag gatcgcctct tgcggttgag
    47221 cagtctaata atatgattcg cctttttgta actgaaacgc ccctttattt gattggcccc
    47281 gagaaacttt taccagccca gtaaacttaa ataaaaaatg ctgatacctg cggcaaatat
    47341 ttgacaatcc tacagccgca ggttttaatc ctgaaaattc tgcataatac gaagaccaaa
    47401 aaccctaatt ccagagaaga gaagaattat gccgaaacgc aaggatatac acaaagtaat
    47461 gattattggt tcaggcccga ttattatagg tcaggcctgt gaattcgatt attccggtac
    47521 ccaagcctgc aaggcacttc gaaaattggg gtatcaaatt gttctggtca attccaatcc
    47581 ggccaccata atgactgatc ccggtatggc agatgtcacc tatattgagc cgctaaatct
    47641 cgaaagcatg accaaaatta tccaaaaaga acgccctgat gctcttctgc caaacctcgg
    47701 gggtcaaagc ggacttaatc ttgcctcgga attagctaaa gcaggggtgc ttgataagta
    47761 tggggtaaag gttatcgggg tgcaggttga tgctattgaa cggggcgaag accggatcga
    47821 gttcaaaaaa actatggaca aattgggtat tgatatgccc aaaagtcagc cggcctattc
    47881 ggtagaggaa gcagaagcaa ttgccggaca aatgggattt ccgcttgtga ttcgtccggc
    47941 ttacacaatg ggaggaaccg gcgggggttt agtttataat atggatgaac tgaaaaccat
    48001 tgccgcccgc ggtatagctg ccagcttagt cgggcaaata ttggttgagg agtcggttct
    48061 gggatgggag gaactggaac tggaggttgt ccgcgatgcg aaaggacaga aaattactgt
    48121 ctgctttatt gaaaatgttg atgcgatggg catccatacg ggggattctt actgcacagc
    48181 tccgatgctg actatcagcc aagaactgca gaaccggctt cagaaatact cttatgatat
    48241 agtcgatgca atcggagtta tcggcggaac gaatgttcaa tttgctcatg acccccaaac
    48301 cgaccgtgtt gtagttatcg aaatcaatcc gcggacttcc cgttcatcgg cgctggcttc
    48361 taaggccacg gggtttccta ttgcgatggt ttcttcgatg ctggccggcg gcttaacgct
    48421 ggatgagata ccttactggc gaagcggcac attggaaaaa tacacgccct ccggtgacta
    48481 tgttgttgtt aagtttgccc gatgggcttt tgagaaattc aagggtgcag aggataagct
    48541 cggcacacaa atgcgggctg tcggcgaagt gatgagcatc gggaaaaatt acaaagaggc
    48601 atttcaaaag gccattcgtt cgcttgaaat cggccggtat gggctgggat ttgcaaaaaa
    48661 ttttaactcg ctttccttgg aggagcttct gcaacggcta aattttccaa ccagcgaacg
    48721 gcagttttta atgtatgaag cccttcgcaa gggtgctgac attgacatac tgcataagaa
    48781 gacacatatc aagaaatggt ttatccagca gatgaaggaa ctggtggatt tagaggaaac
    48841 catcctgaaa tacaaaggca aaccgcttcc cgatgcactt ctcaagcagg ccaagcagga
    48901 tggttttgca gatcgctatc tggcacagtt gctttctgtt tcagaagaaa gtatccgaaa
    48961 acagcgtacc aagttgggta ttgttgaagg ttgggaagca gtccctgtca gcggtgtaga
    49021 aaacgcggcg tattattttt ccacttacaa tgcccctgac caagtcggct catcaaacag
    49081 acgaaaagta atggttttag ggggaggccc aaaccgtatc gggcagggaa ttgaatttga
    49141 ttactgctgt gtccatgccg ctttcacact gcgtgaagaa ggatttgaaa ccattatggt
    49201 caactgcaat ccggaaactg tttctacaga ctatgatact tcggataaac tttattttga
    49261 acccttgacg gttgaggatg tgctgagcat ttatgaaaag gaaaaaccgg aaggcattat
    49321 tgtccaattt ggaggtcaga cgcctctgaa tatagcccgt cagttggagg aagccggcgt
    49381 taaaattatc ggcacaacga ccgaaacgat tgatctggca gaagacaggg atcggttcag
    49441 aataatgatg gaaaaactag gtatccccat gccgcaatcg gggatggcca gcacactgca
    49501 aggcgctctt gagattgctg aaaaaatcgg gtatccttta atggtgcgtc cttcctatgt
    49561 cttgggaggg cgggggatgg aggttattta cgatgaagac atgctgcggc ggtatgtagc
    49621 agcggcagtc gaggtcacgc cggagcggcc gattctgatt gataagtttc ttgtaaatgc
    49681 cattgaagca gaagcagacg ccattgctga cggcacagat gcatttgtgc cggccgtaat
    49741 ggagcatatt gaactggccg gaatccattc aggagattct gcctgtgtaa tacctccggt
    49801 ttcgattccg caaaagcatc tggatacaat ttatgaatac acacgtaaaa ttgccattaa
    49861 tctaaatgta gttggcttga tgaatatcca atatgcaatc gcagacgatg ttgtctatgt
    49921 cctggaggcc aatccgcggg cttcgcgaac ggttcccctg gtatccaagg tgtgcggttt
    49981 cccaatggcc aaaattgcca cccaagtgat gcttgggaag aagctgtcgg aattgaatgt
    50041 aaaacccaaa cggtttctgc atttcggcgt taaagaggcg gtcttcccct tccgaatgtt
    50101 tcccgaagtt gacccacttc tgggaccaga gatgcgatcg actggggaag tcctcggttt
    50161 ggccgactcg ttcggactgg cattctttaa ggcacaggaa gccgctcagg aaacccttcc
    50221 tctttcaggg acggtcttga taacagttgc cgaacaggat aaagcctata ttgctgaaac
    50281 agcgaggcgg tttgcggaga tgggatttaa gattcttgcg acagatggga cacatcgttt
    50341 tctgacaggc aagggaattg cgtccgaacg tattctaaaa ctctacgaag gccggccgaa
    50401 tattattgac cgaattaaaa ataaggaaat ccagcttatt attaatactc ctgtcggcaa
    50461 acagagccaa acagacgatt cctatattcg caaggccgct attcgctata agatacctta
    50521 tatcaccaca gcgacagcag cggcggccac ggccaaagga attgccgcac gattaaagtc
    50581 aaaatcagat gtaaaatcac tgcaggacta tcacaaggaa attctataaa aatcttagga
    50641 cttttaagct cttttatgtc ggggattatc aattaaaaaa tatgatccga aaaggacata
    50701 ttcagatgtc tcctgtcgat tacaggtgat taagcttttt gaaatctctt tactttcaga
    50761 caaagctgga ttacactatt tagtctaaat cgatacagcc aaaaaaatat gagaaaaatc
    50821 aagatatacg atacaacgct gcgtgatggg atgcaggccg agggtatcag tttttccgta
    50881 gaggataaac tgctgattgc gcggaaactg gatgacttcg ggattgatta cattgaaggc
    50941 ggatatcctc aaagcaatcc taaggaagaa gagtttttcg catgtgtgcg tcaaataccg
    51001 tttcgtaatg ctaagctgac agctttcggc agcacgcgcc gtgctcaaac aaagatagag
    51061 gacgatatat gtatccgtgc cctgctggcc agcggcgctc cagccgtaac aatggttgga
    51121 aaaacatggg atctgcacgt tacagatgta cttcgctgca gtctcgatga aaatatagcg
    51181 atgtgcgcgg aatcgattgc ttatgtcaaa aaacaaggcc gagaagtcct ttttgacgcc
    51241 gagcactttt ttgacggcta taaaaataat cccgactatg caatgaaagt tcttcaggca
    51301 gcggcagaag caggtgctga tgcattggta ttgtgtgaaa caaacggcgg ctgccttccg
    51361 catgaggttt tcgatatcac acagaccgta tgcaggacat ttacagggat agaagtcggc
    51421 attcattgtc ataatgattc agattgtgcg gctgccaatt cgttggcagc tgttcgggca
    51481 ggtgctgtcc atgtacaagg gaccatcaat ggacttgggg agcgcaccgg caatgccaat
    51541 ttatgcacga ttatcccgaa tctgtcgctg aagatggggt atgaaactgt cggcagcgag
    51601 cagctgaaaa ccctgacaga agtatcgcgc tttgtctttg aaattgccaa tctgccgctg
    51661 gtaacacaga tgccttatgt cggtgaaagt gcctttgccc acaaggcggg actgcatatt
    51721 gacgctctgc gcaaaaacaa aaaaacctac gagcatatta gtccggaagc cgtgggaaat
    51781 gagcggcggt tcttaatctc cgagctttct gggtcttcga atgttttgga tagacttgaa
    51841 aaacgcaaaa taattacgga taaagcagcg gcacgaaaga ttttgaatcg cgtacaggag
    51901 ctggaaaatc agggctatca gtttgaaact gccgaagcca gttttgaact gctggtgaaa
    51961 aaagaactgg gaacttaccg cagaagcttt gatctgctca aatatcatat tgatgtggag
    52021 cggagtcccg acgggaaaat gattacggaa gcaaccgtaa agcttcaggt ggacggcaaa
    52081 acggagcatg tggtcagtga gggggacgga cctgtcaatg ccctggatgg agctttacgg
    52141 aaagcattgg aaccttttta tccgcagctg cgggatatga cgctgattga ttataaagta
    52201 cgggttgtga atgcccgtgc aggaaccgcc gcacgtgttc gggtaattat tgaaagccgg
    52261 gacaaggatg gctcggtatt cggcacagtc ggggtttcag aaaatattat tgaggcaagc
    52321 tggctggctt tggtggatag tgttgaatac aaactcctga aagcggcgga aaaattggag
    52381 cagacagtct aaattgacct atccgtttta tcttgaattc gcagatgaaa tctgtcttgc
    52441 tcatcctggc tgatgagggg gcaaacagcc aatgcaataa atgaacggat tggctcgttt
    52501 tgaaattaaa aatgttgcag tttgtccaaa atccggtaca gtttgtttat gaatcggttt
    52561 tcggcaagct gttttaatcc tgcaaagata taaaatcaga catagggagg taagaatgaa
    52621 tcgacgcagt tttattgccg ttggcaccgc cgcaactgtg ctgggctcga ccgcactgac
    52681 aactgacaca catgccgctg atacatcagc ttcatcccag cgattcaagc ttaagtatgc
    52741 acccggaccc ggaatgttca aaaatctggc cggtgacaac gtattggacc agatcaaatt
    52801 cattcatgac caaggttttg gggccttttt tgacaacgga ttgatgggca ggcctgccga
    52861 tatgcaggag agaatcgccc aaaccctatc cgggctgggt atggagctgg gcccctttgt
    52921 gctgtacgcg gattttggag tcaaaagctt tgtcctttca tcggcagata cccgaaagat
    52981 gctgacggat aaggctcggc agggagttga aacagccaaa cgtaccggtg ccaaatgggc
    53041 actgatggtt cccggccgct atgatgaaag tctcgaatgg gattatcaga cagccaacgt
    53101 gatcgataat ctcaaatact gtgcggatat ttgcggccaa ggcgggctga ctatcgtaat
    53161 tgagccgctg aatcctcacg atcatccggg gctttttctg accaaaatcc cacaggccta
    53221 tcaaatctgc aaagctgtcg gcagcccatt ctgcaagatc gtcaatgacc tataccacca
    53281 gcagattacg gaaggcaatc tgatacccaa catcaaccgc gcctggagtg agattgccgc
    53341 atttcatatc ggcgataatc ccggccggaa ggaacccggc accggcgaaa tcaattataa
    53401 aaatatcttc cgccatatcc accaaaaagg atatacaggt gttctgtgta tggagcacgg
    53461 cctcagcagc ggcggacgag aaggagaaca ggcacttctg gcggcctatc gggaatgcga
    53521 cagcttttga ggagtctaat tcatgacagc agacattaca cggcggggat ttttggccgg
    53581 atctacagct ggattggcag ccgggttggc aggttcggca tgggcggatg aaccagccct
    53641 gaagcttaaa gggcgcattc gtcaatctgt atgcagatgg tgctacagct cgatcccctt
    53701 agacagtttt gcggcttcct gtgcccgcat cggcctacaa tctattgaac tggttgagcc
    53761 ggcagactgg ccgattctga aaaaacatgg attgatttgc gcaatgacac ccagccatag
    53821 catcaccaag gggcttaacc gcatagaaaa tcatcctgaa tgccttgcta aaatccgcgc
    53881 tgccatcgaa gcggcctcag ccgaaaattt tccaaatgtc atctgttttt ccggcaaccg
    53941 agcaggactg gatgatgcgg aaggattgaa aaattgcgaa atcgctatca agcagatagc
    54001 cggatttgct gaagaaaaaa aagtgacttt atgcatggag ctgcttaatt ccaaacgcaa
    54061 tcacaaggat tacatgtgcg accgaacagg ctggggagcg gagctagtta aacgagttgg
    54121 ttcgccccgc tttaaattgc tttatgatat ttaccacatg caggtacagg aaggtgatgt
    54181 tattgctaca atccgagaat acgccgattg catcggtcat tatcatacag caggcgtacc
    54241 gggacgaaat gaaattgatg atacacagga attgaattac cctgccatta tgaaagccat
    54301 tctcgacagc ggattcggcg gctatgttgg ccaggaattc attccccgcc aaaaagatcc
    54361 gctggcctcg ctggctaaag ccgctgaatt gtgcgatatt tgaagaaaga ttcaattgta
    54421 tgaagaaaaa aatattttct attgtcggac tcattctgct ggcactttcc ggctgtgcat
    54481 ccaaagagga gcttttcaat ggccgaaatc tggacggatg gaagccgtat ttggcggacg
    54541 cctccgttga tccctcaacg gtctggtctg tcaaagatgg agtacttcga tgcgaggggg
    54601 tacccaatgg ctatctgagc actgtccgca catacagcaa ctattcgctg catctggaat
    54661 ggcgatgggt cgaaacgccg accaacagcg gcgttcttct gcatatgaat gggccggata
    54721 agttatggcc gcaatgcatt gaagcacaat tgaagcatca ggacgcaggc gatatttatt
    54781 taatcggcgg aacggattgc aaaggaatca ctgaccggca gggttctcgg cttccgaaaa
    54841 tcaatccgac aagtgaaaat actgccggcc aatggaacag gtacgatatc gtatgccgcg
    54901 gcgacacaat cgaactgcat gtcaatggca tccttcaaaa caaagcaacg caggcatcgg
    54961 ttacatcagg aaaaatttgt ctgcaatcgg aaggatctcc gattgaattt cgcaatataa
    55021 aactcaggcc tttataatta acaaagggcg aaaacccgaa atcacagtta aaggagtaca
    55081 caatgaaagc aggtttgaat cgcagggagt ttgttaaaag tgcagcggca gttgctgcag
    55141 gcacattggt ttccccaacc ggattatttg cggccggttc agaaaccatt aaagtgggat
    55201 tggtcggctg cggcggacgg ggcaacggcg ccctgaatga ctttctgaat gctgcgaaac
    55261 atttgaacct caaagtggaa atcgccgcat tagctgattt cttcaaggaa agggcagacg
    55321 gcacagctga gaaattcggc gtttcaaagg atattgctta cggcggagca gatgcctata
    55381 aaaaagtaat ggcctcggat gcacagatcg ttctcatggc tacacccccg atttcgcggc
    55441 cggcacattt tgaagcggct gtaaaggcgg gtaaacacat ttttatggaa aaacccgttg
    55501 cagttgatcc gccgggcgca cgcaaaataa tcgcaatggg caaggaagca gcccagaaag
    55561 ggctttcgat tgtagctgga acccagcggc ggcacgaagc tgcatacctg aagaccaaat
    55621 atgcaataga ccaaggcgcc atcgggccta ttcggggggg ggcaatctgg tggtgcggcg
    55681 gcgctctttg gtataaaaag cgggaacccg gcgaaagcga cgcatcttat atggtccgca
    55741 actgggtcag ctttacagaa atgtccggtg atcatattgt tgaacagcat gtccacaata
    55801 tcgatgttgc caactggttt atcggcagaa cgccggaact ggccataggc ttcggcggac
    55861 gtgctcgccg gaaaaccggc aaccagtttg acttcttcag tgttgatttt gattacggcg
    55921 acggggtgca tatacacagt atgtgccggc agattaatgg gacttacagc cgggtctcag
    55981 agtactttac cggaactctc ggcgggacat ggggatcagg ccccggcaaa gaaggttttc
    56041 ccaaaaaggt tgacattcct gagataccgt atgtcggcaa tccttatgta caggaacatg
    56101 tcgatctgct cgaaagcatc ctgaatggaa aacccattaa tgaagccgag aacgttgcta
    56161 catcgacttt gaccggcatt atggggcgca tcagcgccta taccggccaa ttggtccgat
    56221 ggaaggacct tgtggacgag acaaccggct ctccatggta taatttaacc ctcagtccga
    56281 cagaggcaga ttttgaaaac aacaccgtac aggcccctgc cgatgagatc tgtgctattc
    56341 cgggcaagga gtgaacaatc ttttttccac agtcaagccg gcggatacat atccgccggc
    56401 ttattttttt ggttatactt ggatgacgtc tctgttagtg caacacaaca tccaaaggga
    56461 ataggcagga acagcttaat agtactatca atagttttcc gcaaatttgt taagcagacc
    56521 tttatctgga ctgtttacat cggaatcaac ttcaaataat attgtcacaa actgaccttt
    56581 tatatcagca ataaacagcc ccactgcgtc caatcgatgc ggtaaccttc aaattacact
    56641 tggggcaaaa ctattgaccc ggtgaaggtt ctatttctcc ccagctggct ggattgttgc
    56701 cgctgccggt aattgttatc cgtcccagag ccatccctgt tccatccggt tctgcaggcc
    56761 acggccaagc atcgccataa atgcattcat caacaattat ccaggaaata gccgtcggaa
    56821 caagtggatc atccgaatcc tgcggttttt ccagcgtgag acgctcgcct gcatttgaca
    56881 gatcgcctgt ccaagggccg aaaatattga ccccggccac taaatctgtg ttgttgtaaa
    56941 ccgctttaaa cgcattcaag cgttgtgcat ccgccgcagg atcaaatcct acaataacaa
    57001 tcttggtctg cgcaggcaac tgaattgacg gagaaaattg aaagttgact gccccatcca
    57061 atgcccacgg ccctgctgaa gtctgcagct caattgccgt atcggtggga ttgtacaatt
    57121 caatatattc atcttggttt gtgccgtttt ggggatggta cataatttcc gatataacaa
    57181 tacttggaag cggctggata ttgggagcat ttcgactcgg ctgcatcgcg agccaatacg
    57241 gtcctgcatc cggatagcgt ccatacgaga agccgttttc ctgtcctttg aacttgatgc
    57301 aatcaacaat cctgtcggca tttggaacgc cgggcagata agacaggaat atctgttcgc
    57361 ccgccttatc caagccgaat cctgtggaaa ggggactgtg gaaaccggtg acttcgtcaa
    57421 aactgataaa ttggccggat aaaacagacc ggaccggaag cggccacttt ttcagattat
    57481 tcccatcatc actgagatac caattgccat ccagactcat cgaatttcct gcattgtaga
    57541 gttcaatcca gtcgttgctg tcatagccgg gataattctg gtcagaaaaa tcggtatggg
    57601 ccataaactc attaataagc agccctgccg gcaacgaggg atcatcagcc gcaggagatc
    57661 cgccgatata ggcactttgc cgccagttgc cgccataatt aagcgtactt gatggccggc
    57721 ctacaacaat cgcattcggc aaaggcacca gcgaatgacc ggttccatct gccgcaatcg
    57781 gccaaccacg gccgtcattg tattcaaact cggctaacgt tccattccat gtatcttcca
    57841 gcttaatcac ttcgccgctg tttgaaagcg agccggtata ggttcccgca atccggctgg
    57901 ataatcccgg ataacgcgag tcgaaagccg ccgcattctt taccaccaat acaaactgac
    57961 cgggattgag agtggtaact gcactgccgg cgaaactgaa ggtaacgccg ctggtgaatg
    58021 agacagcact taaatccaga attttatcaa cgctgcaatt tttgagttcg ataaattcaa
    58081 attcattgtt atcgacattt aattcgttga gtgcaggatt ggcagcggcg ggattgtaca
    58141 tcacttcggt aatccgcaga taatctcgga tttctgaacc aataggctcg ccggcaacaa
    58201 actcaatcgg ggccgaccaa tgactccacc gccctgtcgt atctttcatc ttgcagcgga
    58261 cccggtaggt acggccgggt tttacaccat cggcgggaat ttgaatatct gaattgaagg
    58321 tggtcaattc accgctttcc cagaccggct gcagctcgta tttatgccga gataaacctt
    58381 ttttcagttc ctctgaaggg gcagacaatt tggcgatcac ttccaaatct gcatagcaat
    58441 cactgctgcc gctaagcgag acattgataa cctgaattgc tatgacattc gtcccggctg
    58501 ttagatactg ctggggattg gtgatttcaa aggatttata aattgtttcg tcagggatat
    58561 aactgagccc ataggactcc aaaacggtat tgtaaggcag attttcttga gccggaacat
    58621 tttcctccag cgccagctgg ccgttaatcc acagcttaaa tccatcatcc cgcaggatag
    58681 caaactgaag agactgaatc ttttccagat cagcaaccgt aaaagccttt cgcagataaa
    58741 tcgtgctgta attgcctctc atatcattca gcgatgtacc catcacaaga tttgtgtcat
    58801 aacccacagg agctgtgcct tcggaccact gactatcgtc aaatcctatc tgccgccatg
    58861 caccggtttg tgcggagggt tcctgggtac ccttgaaata gcgccataaa gcaccccgat
    58921 ttatcaaatt tatctgggtc ggagttgaag ggcctcccaa attttcatat tcagcaatgc
    58981 gccacttcat tgctgcaaaa gtaccattgc cttgcggacc gctgaacgcc gatgtacgga
    59041 agcgaaggtc attttcagga taccctggcg tgccaatgta tgtaatagtc ggagtgtttg
    59101 gaatattcgt cgaatcgcct tcggctccgg ccaaggtatc taaattgctg gcggtaccgg
    59161 accagtagta tttgccgtca aatgttccag ctgaccaggc aaattgctcc atcagtgcca
    59221 cgccctcttc cagaggccca ttgtcaattg aattgctgac cagccgccag cggtcgcggt
    59281 cggcaggtac aaaatccgca atcacagaag ccagttcagc gataagggga ttaatgactt
    59341 ccggctgcca taaaagatcc cggaattcgc gaatgtagtt gcggtattcc tgcttgattg
    59401 gcgctttttc cggcttggct gtatattgcc gaaggacgtg atcgccatcg tacatctggt
    59461 cataaatggc gcctttggca tgatcgcagc cgtaattaaa atagggaccc catgtatcat
    59521 ctacatcgaa tggaagaaat tggagttgtc catagggatt ttctgcggag taatttggat
    59581 aaaaatacca cgcacagttt ttcagacagt gaatgcaggt tgaaccggag aaaatatcat
    59641 aatggcggat ggcttctgtg acggtatggt agcggtacca ttcatcacaa tccagatagc
    59701 ggcggataaa atctgcggag gcctggtaat tcagattcca ccgtatattt tcatagtccg
    59761 aactgtcggc cactgcattt tccccttgat agcgaagctg gcgggcgccg tcatatatct
    59821 tatccgacag tttatacaga tttgctttcg gcagattgag ccgttccaga aaggcgccgt
    59881 cgtaattttc gaatgctaaa taaagccccc agaagtctcc ctcatattgt ccctgtgttc
    59941 ccgccggtgc ctcttctaat ccatcgacga cgcggaagtg aatccagtag gtttccgggg
    60001 caggcacacc agcgatatta aacagcagca tatccatcac ttcattgagg ccgtaaggat
    60061 atttacggta ttctgttatt ttatttccga acattttgcc tgtgttgaga tgctgccatt
    60121 tcgaaggaaa tggattgccg tacaggtctt tggcctgaaa ataattgccg cgattaaagc
    60181 ggaatttcat ggagcgtttg cccccaagac cgtaattgta acggccgttg ccgccccgca
    60241 aacgatagcc gatatggtca tagaccttgc cctcatagac aaaggcgcct tcccagttat
    60301 agaccctgcc ggcatcctgt gttttaacat ctgttgtccc ctgatcgatt cgatcggcgg
    60361 cgttgtagcc attgcattga tataaatccg ctgcacgcgt aatcagcgta taaacgggta
    60421 tagacgtaag aatatctgaa ctataggaat ggccgacacc atcaggatgt accgaagcgt
    60481 gtgtcgtggt ataatccggc acaccgtcat atataaaaca agcaaaatta agagaaggat
    60541 catcggcata cggcacgcgg atggaattgt cgttctggtc ccacgcctca atgcggtacc
    60601 gaagcaggac tcggttggac tggggcgaca aacgacaggt ataaatatta tcgccggcaa
    60661 ctgcatcacc aaacaagccg ttatccatca tcagcacagt aatccaagaa ttctcaaatt
    60721 ccggatttaa cggctgaggc tggtagggat cggctatcag ggatgaaatc ggaattggca
    60781 gataggcggg aatataggag ccgggcgata ccagctgata gtttaaataa actctcttaa
    60841 cgccgtccgg gtctgaaatt ttggcggtga ttagaatcgg ttctgttgac cgcggctgct
    60901 ggggagtatg gcgaacctga cgaatctggg gaggagcgtt gagggcatag acgctgtttc
    60961 ggcgtccggg ggttggtgag ccgtacgaca attcaggacg tcctgcctca ttgtagccgg
    61021 agctgcgcca gcttcctgcc aagtcattat ccagcgacgg atgaatcagc tccattgaag
    61081 cgccttcgcc gccagcagca atcggccaag gaaattcgct gtcgtaattg acttcgtcaa
    61141 tgataaggcc ggtcgggttt cgcagaacaa gacgctcgcc ttcattgctc agcttgcctt
    61201 caaacggccc ataagccggc aggccaaact tgatctgaaa tgcctcggga ttttcactaa
    61261 caacaatata ggcaccggga tgcatcaccg gcacattgcg gaaggtatat ttaacggccc
    61321 catcaagacg ccagccgttt aaatctactg acatagcaga agcgttgtat aattcgacaa
    61381 attcgacagg ttccttgttg ctgtctggat ggtagtggat ttcattgata accacttgcg
    61441 gataacgcga atcaagccag ttatcggcta aaactgtaag gtccgcagta tcaacccaac
    61501 ggttttggtc ccaatccggg caatcaaagg cactgcagtc tatattgctc atccagtatg
    61561 aactgaacaa tgcggcatcc tgcagattta cctgacagtc gccatctaaa tcgccgccca
    61621 aacaggatgc gggcttttcc attgtggtaa atgtccagac atcgccgata attgtctcag
    61681 aaggatagat ctcatcaatc cgccaataat aaaccgtttc aaagtcgagc ttcggcagcg
    61741 aataggatgt cagcggataa tttatctcaa gcgtactcaa agccgccggc gaagtacccc
    61801 acagaatttg atgaccgaca gcgccggctc ctgactgcca atgcagtgtt actccttctg
    61861 ttccaactcc ctgaacaccg tttgaaggaa acggggtatg ggctttcaaa ttgacagtac
    61921 ggaaattcca gaccggcccg ctgaacacat agccgctgac tgtaacctct tctattcgcc
    61981 agaaataggt ttgatccatc atcagtgcag cggctggagt atagttttcg cagccggcgg
    62041 gtctaacacc ttggaaaatc cccgctgatt caggtgtggc cttcagaaca ctttggtagt
    62101 ccgttcctaa atagacgcga tgaccggtga tatcggctga tgtgtccgac tgccatgtca
    62161 ataccggcgt caatccgccg gaaattccgc cgctgtacgg ctggggatta gatggtgccg
    62221 cgtatgctgc aatcggggca tcggggccgg cttggtaact ggccagcagc attgcatcac
    62281 tcagcgcgat gtcatagata cgaaactcat tgtaactgcc gttaaatgcc gggtccggcc
    62341 actgggaccg tcccagccag ttattgacat ctttcaactg ggataacgta aacaaaagcg
    62401 gaccgctggc tacccgctgg ccattataat acatccgtac ttgaccggct acatcatccc
    62461 agacaatggc tacatgcacc tgctggttga ccggcaatgc agcagaatcg atggtcaatt
    62521 caacaggtgt ccccaattcg atgccgttac ggtgaccaaa acggtacgtt ccgccgccgg
    62581 aatacggagt caggaaaaaa taccctgaat tggcggagtt gacagattgg ttttcgccgg
    62641 catcactgat tccaatgtca aaaatccttt gccaagcgga attggatggg ccgtaccaag
    62701 taacccaagc ttccagcgtt actgccgggc tgattgagga aataatgcca ttgggcaaat
    62761 caacataatt gatggtagag ctgcttgaat tgggaagttc agaattaccc atttcaagcg
    62821 tcgtactggt aaagtaggca tcggctccat tattgacaag cacaccatgg gcattgccga
    62881 tagaatcgtt tgcatttcct gaaaaactgt atcgatgaat catccctcca taggccgcta
    62941 tggagagcat acttaaaata cctgcgatta acctatgtaa aaccggcttg ctgcttctca
    63001 tgctcgtcat cacccctata cccttaaaca aaatataact ctttttgttt ccaattaata
    63061 taatacagaa tatatagaat cttctttgtt tgtttcgaat cgaatacaaa caccctgccg
    63121 cttaaagcac agataaagca ttctacctct ctagttatgc ttttaccccc atatgccgaa
    63181 attacaaggt atcattgtta ttatatccat tactgttatg gaaatcaatt tcatttacca
    63241 ttttagactt aaaatacgca tttttattga aaaatcctgc aattatgaag cgatataaat
    63301 accatttgcc cgacggttat attcagacgt ttgtaaatct gccgggtaat ctctgcctga
    63361 atagattcat ttgtatcaag acgtgcaagc tcgactttta cggtcggtcc tacagcggct
    63421 acatgaagaa tccttgcttg aattgaacct gtatcaaggg cggtttttga gatttcaata
    63481 tcgtgaggcc tgatataaga agctgttgca gaaggttgag tcgattgact gccaaacggg
    63541 cttcgtagaa tattcacatc ccctaaaaaa ctgaaaacaa atggattagc gggattatcg
    63601 taaacttctt ccggtttgcc tatctgctcg atccgaccgg cattcatcac cacaatccga
    63661 tcagccagtt caagcgcttc ttcctgatca tgggtaacaa aaacgctggt tatggacatt
    63721 tcatcatgaa gccggcgaag ccaatgccgg agctcttttc gaactttggt atccagcgcc
    63781 ccgaacggct catccagcaa aagaattttc ggctctacgg ccagcgaacg aaccagcgcg
    63841 acccgctgac gctgcccgcc ggaaagctgc gagggataaa ggtctgcatg acgttccaac
    63901 tggactaatt tcaggaggga aaaaacgcgt tccctaattt gggacttgct gagccgatac
    63961 ctccgcggcc gcacattcaa gccaaatgca acattctcaa aaatagtcat atgccgaaac
    64021 agagaataat gctgaaatac aaatcccacc cgccgctgtc gcacactttg actgctgaca
    64081 tcgtcctcat aaaataaaac cggcccgctg cccggatccg gcgactccag accggcgata
    64141 attcgcagca gtgtggtttt gccgcagccg gacgggccga gcaaggccac cagctctcca
    64201 tgactgacag ttaaactgac attttggagg gccttaaatg ttccaaaagt cttgctgata
    64261 ttggtaattt gaatactcat tcgatacctc cgatgaccgc ggcggcttgt tcgtttctgg
    64321 ttttccattc taccagcgac ttcacggcaa gcgtcagcaa ggccagcagc gtcagcagag
    64381 aagccatagc aaacgcaccc acaaaattat actcgttata gagaatttca atgtgcagcg
    64441 ggatcgtagt cgtctgaccg cgtatatggc cggaaactac cgaaactgcc ccgaattccc
    64501 caattgcacg ggcattgcac agaataatgc cgtatagcag gccccatttg atattgggca
    64561 gggtaatacg gcggaagatc tgccatcccc ctgctcccag caccatagcc gcttgttctt
    64621 cctctgtacc ctgagactgc atcagcggaa tgagttctcg tgcaataaat ggaaacgtta
    64681 caaacatcgt tgccataata atgccgggcg ctgcaaatat tatcttgata tcgtgttcca
    64741 agagccatgg tcccagccat ccgcgagcac caaacagcaa aacaaataga agtccggaaa
    64801 caaccggtga aacagcaaat ggcagatcaa tcagcgtcag cagaagacct ttgccgcgaa
    64861 agtcgaattt ggcgatggcc caggccgctg caaggccaaa gatgaggtta aacggaacag
    64921 ctgtcagtac aaccagcagg gtcagacgga cagccgatcg tgcataggga tcggcaaatg
    64981 tctgaaaata gggacctatt cctttgcgaa acgcttcgat gaaaaccgct gccagcggca
    65041 gcagtaaaaa caagcccaaa aacgcaagtg cagctgcagt cagcactatc gggatccaag
    65101 gtaatttatt tttcatttgg cctccgtttg cctgctctgc agcatccagc gctgcataag
    65161 attgatcgtc aagagaagca caaacgacag cagcagcatt ccggcagcaa tggctatggc
    65221 accttcatag tcatactgct ccagttttgt gataatcagc agcggcgcaa tttccgtttt
    65281 catcggcata tttcctgaaa tgaacacaac tgaaccgtac tcgccgatgg cacgggcaaa
    65341 agccagcgca aagcccgtca gcagcgatgg aagcagttcg gggaaaataa cgcgtctaaa
    65401 tgtctgaaaa ggagttgcac cgagacttgc cgccgcctct tctaattcct tcccaatatc
    65461 ttcaagaacc ggctgaaccg tgcggacaac aaagggcagt ccgacaaagg tcatagcgat
    65521 aacaatgccc agcggagtaa aggccgcttg tatgcccagc ggctccagca ggcgtccgat
    65581 ccagccggtt ggagcataaa gggttgttaa cgtaatgccg gctacagcgg tcggaagcgc
    65641 aaaaggcaaa tcgaccatgg catcgaataa ttttcgaccg ggaaaacgat accgcaccaa
    65701 cacccatgcg gtcaaaaggc cgaacaccgc attgaccgca gcggccgcaa acgatgtagt
    65761 gaaacttaac cggtaggaag ccagcacacg cggggaggtc atcgccgccc agagctgatg
    65821 aggttccatt cggacggcag aaataactaa ggctgatagc ggaatcagta caatcaagca
    65881 caaatacagt gccgaatagc ccagagtcag cccaaaaccg ggcagcacat ttttaggttt
    65941 atactgcatc tttattgttt agaaaccggc ttattcaccc ctcttccttg ggcagccgcc
    66001 gaaccgctgt ttatttaact ccataaatct gatcaaacac accgccatca ttgaaatgcc
    66061 gttcctgagc ggcctgccat cctccaaaca cctcatcaat agtaaatagt ttgatttcag
    66121 ggaaaaggtt cttgtatttt tgtgcaaccg ttttcatttg cggccgataa aagtgcttgg
    66181 cagctatttc ctgcccctgt tctgaataaa gatattccag ataggcctga gcaaccgcca
    66241 gagtgccctt gcgctgggca actttatcaa ccaccgcaac ggggggttca gccaatatgc
    66301 tcaaagaggg tattaccatc tcaaatttgt ctgctccaaa ctctttgata gccaggaagg
    66361 cctcgttttc ccaagatagg aaaacatccc caatgtttct ctgaacaaag gttgttgtag
    66421 aactccgcgc gccggaatcc agcaccggta catttttata aattttgctg acaaaatccc
    66481 gagcatatgc gtctgcttta gatgcattct cctcatcggg atgagcagcc cgatactttg
    66541 caagagcata tccccaagca gccaagtagt tccagcgtgc tccgccggat gttttgggat
    66601 tgggtgtgat aacggagata tccgagcggg tcagatcgtc ccagtctttg acatttttcg
    66661 gatttccctt acgcacaaga aatacaatgg tcgaggtata aggtgaactg ttattcggga
    66721 ggcgagattt ccattgctta tcaatcaggt tccctttatc cgcaatagca tctatatcat
    66781 atgccagcgc cagtgttaca acatcggccg gcagtccgtc aataacagcg cgcgcttgtt
    66841 tgcctgaacc gccatgggat tgctgaataa tgactgtatc ttgggtttta actttccaat
    66901 agtcggcaaa ggccttgtta tattcctgat acagctcgcg cgttggatcg tatgaaacat
    66961 tcaggatagt aatttccttt gaaaacgccc aatttccgat acccagaatc atcatccata
    67021 atacagatat gagatatgtt tttttgttga ttttcatagt aaaatccctc ctatcaattc
    67081 catattcaat ctcaccgtta aaatttgatt gtaaaatccg caagcacgac atcataatcc
    67141 gttgttttcc cgctgcggtt acgcttggca tccatatatg acaagccgat ttgggtattg
    67201 ggagccaatt ggtagctgaa gctgtattta tgccccttta aatcagtccc cccgccacca
    67261 aaggtcgctt ctgttagtac accaagaaga gcatcggctt ctatatcccg atagttataa
    67321 gcaaaggacc aagtgccttt ttgcgcggcc tttcctatgc ttgctccagc cagccatgca
    67381 ttgttttggc cggactccgc accggtattg tgcgccaaat cgccgaagac acgcagaggc
    67441 agactgccca taggcactcc aatctccgca aatccctgaa taatatcaaa gtcgctgaca
    67501 tagtagccgc caatagcctt attgccgtaa aactggctgc tgttggcacc taatccttcc
    67561 cttcctttaa cattgccaaa atcataatac cccccgccgg ctgtcagata ctcatcgctg
    67621 tgaccaaatt gccatgtggt gccggcctga acagcccata aactgctgtc ggcttccgtc
    67681 tggcgttctt cgagccagta gcctccaacg gttccaaata cctttatagt actggatacg
    67741 ctggtactat aaacaccggc tattccctct ggggtaatat ctgtatcaaa catcaaatca
    67801 ctgttgccaa ccatataata cggatttttt atcttgccgg cataaatatt cagtccccca
    67861 attcctgccg gatgataatc cacataggcc aaatccagcc agatgttttt gctggaaaag
    67921 gcattgtcta aatcctgatt attcgatgtc ggcgatcctt cccccaaggg ggcttcgctg
    67981 ttgccgctgg ctaaccggaa tccataagaa aactcctcat tgacagcatt agttaatcca
    68041 attcgcgtct gaatgcggtt tctgttgcgg tcggctgttt tggaggcatc gtctgtccat
    68101 tcatagcgat agcgaaaatc tccgaatatc tgcattttct caagccactg aggcacagca
    68161 aaatcctttt ttctcatttc aagccgctgg tcaacaatct gctcaatctg ctttctttca
    68221 tcaggttgcc cagacgagtt atcctgcaga acaacagcac ccgacgcgat ggtaccttgc
    68281 gcttgcataa gccggtcaag ttcggcctgt gtttttgcga gctgttccgc aagataggcc
    68341 gttcgctgcc gctccatttc aagctgtctt tccacatcta tctgtttttg ttccagctgg
    68401 ttcagacgtt cctccaatgt cttggtatcc tgcggagaat cggcatgagc cgtacacaaa
    68461 aagagggtga aaacaaggat taaaaaagcc aatacggtac tgtcgtcttt cactcttcag
    68521 tcctttcctt ctttttcttt tgttttataa cgaccatttt agtcgtatta tagaagatag
    68581 aacacctcta aatacctgca ccatctgtaa actgggctgc caatgcttca tcatcctgaa
    68641 ttaacatgtt ctgaaataca tccgccaggg aagtatggtc cagccgttta gccgcatagt
    68701 tgcgaacatc cagaaagagc gagcgaaacc gacaaacagg ccgctgatca caatgttcat
    68761 aatgcctcaa agaaacgcag ccaataggtg ccaaaacacc atcaaagtgc cttattacct
    68821 ctccggccgt aattttgtgg ggcggcttag ctaatatgta ccccccctgc ttgcccattg
    68881 tgctggctac ccaccctttg gctttcattt cgagcataat atgctccaga aaccgtttgg
    68941 ggacattatt gcggcgggca agctcacgaa ttggaatggg gcctcgctga taatgctcta
    69001 ccagagtaaa caacgctcga aacgcataat cggttttcat cgatattttc atcgttaccg
    69061 cctatatacg actgaattag taatatatta gcaaatatga ctgcaccagt caatacctaa
    69121 atgtaaaaaa agcaatcagc tgatctttac gataaataca aagacaaaat caggcatgaa
    69181 agaatacaaa cgggtaaatt ctatgtcttt gaaaataaat gagttactgt ttttttttac
    69241 cacaagctgt tctctgtgat aagcatcgta tcggctgtca gtagcaatac caattacctc
    69301 gtaaactttc tggatttcat tttgcgatat aagggcaatt gaacaacgaa tttgtacagg
    69361 ggattcatcc accgttaaaa tcactgtttt ttctaaatca ttcagtttgg attgatgaac
    69421 agacgcccaa gtcaaaccgc tcctgcaggc atctttggcg tgctgctgga gctgttccat
    69481 tcgaacctga tagcccagaa ttttggaatt tgccgaaaaa atcgccatag ccatccccat
    69541 cagggggatt agaacaaaga caatcattaa gataaaacca ctatttcgct tcgtattcat
    69601 aaggcctttc attgctttgc cgcttggcac atggattcaa attattatca atcaggatcc
    69661 ttgaagttaa ccatccattc aggccattga ggcagaggtt ttgaagtatc aaaacctttg
    69721 tgccatttga gccgccacaa ttccttaggg cttactgcag aaacatcgcc attaggaaaa
    69781 agcacattga gcatgtttcg atgccaatcc acacagaatc gctgcatctg gttgtcaggc
    69841 tgggcagccc actgggtatt taataaatag tctctgcttt gcggaggtac atcactatct
    69901 tcaggacgtc cggtgtacca taaggcatcc agaaacagcg ggattttata gccgcccact
    69961 acatcgattg tccgccagct gtattgggta tcatgtcctt ctgtctggat aacttcaggg
    70021 ggagtatcgc agacccaccc atttaatcca tagctgcctg aaagaatagt ctctgcctgt
    70081 cctgattggg aagtgctttt tcccgttccg tatgtttgac gtgcagccgg acaaaacttt
    70141 agtttcgggg tatcataata cgcctgcatc gcatacggcc acaggtctgc actgcggctt
    70201 tgcagtaaaa cgccgcttgg atcagcatag gaataatatc ctgcaaaaaa gcggccgtcg
    70261 tggtcttcag cgtacaaggt aaacaattct ccccactgcg acagattagc catacatagc
    70321 acctttcttg cctgttctct ggttgccctg agagccggca gaacaatcga catcagcata
    70381 gcaatgagac taataacaac cagcagttcg actaaggtaa aagcagtttt cttgcgatgg
    70441 acatttgctt tcatggctta tatctctttt ttgataaagt aaacatgtgt ttgttggaat
    70501 ttctgtttgg attgaccaag gataggccgc tcgttccaga cagcaagttc cagagcaatc
    70561 ggcccctgtt cgtttttcca tacaatccat ttaagataca cattcggcag ttcccagaca
    70621 tcttcctttc ggtccctcgg cagattggat tgacgaacga ctttgccgtc tgccaagcaa
    70681 taactcacta atccgccggc ctgcttaaga ataagcagat tgctgctgat gcggggatcc
    70741 atctctatga cagataaatg atccgaatgc tccacatcct cttgaagatg cttgagcata
    70801 tgaagcgtct gcgtcagtgt ttgatagtcc ttatagcttc ggggcagctc tgaaataatt
    70861 tggtgcatcg gtttggtcag cagtgccatc ataacaacaa gtgctgccag cacagccagc
    70921 atctccatta ttgtatagcc cttgagcttc actgtttgag ctccttttgt gctgcaacat
    70981 agcggaccaa agaggtctta accgttttcc ttcctgattt tgcagacaga gtcagctgaa
    71041 ttctttgaag tccctgccat tgcccctccc cctcagttat gtcaatacgg cagtcaacat
    71101 tcggccagag gcaggcaaac gtatccttgg caataggttt tccagtaatg tccaaagcat
    71161 ccatctgagc ctgcccagca gcaagcagcg tatgccgaat ccagcgcagg tcatttgctt
    71221 tcttaaatgt acccccgaca gctgccagag cacctatcag gattgtcagc acactgatag
    71281 cagttatcaa ttcaaagctt aacacgccaa accgtttttt gtgcgcgtga aatctaaaac
    71341 taaaattttt cataaaaaca tctcgattat tttcaccatc ggcaaataca ttcctgcaat
    71401 cacaaatccc acaaaacaac caagacccac aattatcaat ggacagcata cagcattcat
    71461 cagattgatt cgatagtggt atcggcttcg gtatagatgc tcgagggcat caagaatctg
    71521 cggtgtattg cccttattga tagattcatc aaaggaccaa accaaggcct tgtcaaagcc
    71581 gcactgctgg gcggattttg caatattttg cccctcctcc actcgactaa gccactgctc
    71641 gagccgtttt cgaaaacacc aattcatatc gaggtccagc gcattttgaa ttacagtatt
    71701 gacaggccag ccggcctgca gccctacttt cagcatttca atcagctgca gatgtgctcg
    71761 cgttttctca aaccaatgta tcacaggcag atgccacttg aaccaatcgc tcagacggtt
    71821 tgtcagacta agacgcccgg acctgcggcg ataaatactg ctgaatacac aacgtaaaac
    71881 aatcagcaga ataacggcgg caataacagc tgcattaaaa cctcttggcc ccattataaa
    71941 ttgcactatt tgaaacagta tctgcgtgac ggcaggcaat gctgtctgat gatcactcaa
    72001 atcttcaaga agctgtgcaa attggggtat tataaaaaca gccaaaccca atacaaaaaa
    72061 aactgctgca atcataacca gcagcggata ccaaggatga accggccgaa ctcgtctgta
    72121 attgtccact ctttcggtaa tatctgccaa aatggtttca atagcattcg gaagctgatt
    72181 cattttttcg ccacagcgaa cagcagccag cagttccgga gggcacttgg gatagccgcg
    72241 ggtcagtgcc tccgagagcg tccagccctg cgtcagccat tcagcaatat ctctgaagat
    72301 gcgggaccgc cgtttttttt gaccctgtgc tgcagcgttc aataccatcg gcagcggcaa
    72361 cgactgccgc atactcattc ggacagccga cagaacatcc agcgccagcg tatatcggca
    72421 ggtcagcttg tagcgaacaa ttccaaccac aacaagaata aacagaacaa ttcctaagat
    72481 gtgaaatact gctgttaaaa gggctaataa aatcagatat ttgaaaagaa tgataacaat
    72541 aatagcaaac gacttgtacc aaggagtttc cagatgaccg gctgtcggga ccaatcgcac
    72601 cagtacaata gatgcacaaa acagacctat tgccatcaca acgatccaga aagcctcgtc
    72661 agtaatacca gcaatagcac ctctgtcaaa atcaattgcc gacaggatca taccagccat
    72721 ggcaaacggc agtaaaatca gtcccaaagc cggttttttc cagatcagaa cgcagaagac
    72781 agcgaaaaag attagatttc ccagataaag catccacata ctacatcaaa acttgagtta
    72841 tttttaccat cggcagaaac aaagacaaga taatcagacc aataatgccg ccaaggagga
    72901 taattagcag cggcatcagt acggcctgaa gctgggattg gagggtatag gtcttggcag
    72961 catacaattg cgctagcccg tccaaattct ccctcaattc attccgctgc gatccgagct
    73021 gcaccgaata aagaaacaaa tggctgatta cggagcagtt caggcctgcc tccattacat
    73081 taaagccctc gcgcagctgg ccggccagca gaacacagtc ctgcttgagc cgctcgctgc
    73141 cggaagattg tccggccaat tcgactgctg tatccatcgt acagccggcc ttgattaagg
    73201 ccgacaggga ttctgcaaag cgggacatca atccgtttcg ataaacccgc cccaaaagcg
    73261 ggatattgtc cagtactttc tccttaattt gccggccttc cggagaagcc gacagcacta
    73321 tccagaacaa aatacaacca cctataagga tgccggctcc aatccaaaat gaccagatat
    73381 gctcggccag atttaaaaga aaaactgtca gcatcggaag ccccaatctg ccatccgaca
    73441 tgtcccacat caattcggcg aaagcgggaa caaccattgt aaaaagaaac gttaccagaa
    73501 ttgctgcaat cagcaaaatc atcagcggat aggagattgc ctctatcaca attctgcggg
    73561 tcctatgctc aacctgcaaa tggcggttta agttcgccag catctcatgc agccggcctg
    73621 tttcaatgcc tgccttaaat atcatattgt acagcggcgg aaagtgtttc tgccgttttt
    73681 ctatggcttt gtcaatcgga attccctgct caagatcatc ggcaatgctg ttgagtaagc
    73741 ttttcattcg gccagaacct gcatcgtggg ccagttcacg caatcctcgc tccagcggaa
    73801 tgccggcctt ggtcagtgag gcaagctgtt cattgaagag cataaattcg ctgcggccga
    73861 caggatgcgg agccggcgcc ggcttggcct gctccagttc ggtcacttgc aggcccagtt
    73921 cctccagcga tatccgggcc tgatctatat tggccgcttc aatggttccg gtcatcagac
    73981 gccctgcttt cgtacaagac cgatattcaa ataatgccat agattattcc tttgcatggg
    74041 acataaggcg gattttatat cgaaatgccg acggcatcgg ctcgcggagg acgtcggctg
    74101 tctggaggat ataggcatcc tcctgcctgt aataaatcag aggtcttttt gttacaggat
    74161 cacagggcag acgtatttct gttatctcac ccagtgagtt tggtaaaagg ccatcataga
    74221 cagatgcata atatcgcaat ccctctatgc attcgagaac cgcaataaaa cgatcgatct
    74281 gctgacacat ttgttcggct tgtctgctcg tatagacagg cagggtttct tgttgcgtcc
    74341 tgatctcatt gacatccggt attgctgcag tgccatcgga tagcatctcc atcatcggat
    74401 taaactgctc ggtcttcttg cgatccggtg catctgtact gtctgcgact aaaaaaggca
    74461 tcggcaaatc ctgcagagac cgatagagac ttggtgcacc gggagtctga atccatagtt
    74521 cgatctgggt gagcattgca gcagcgttgg cggctgccat catagccagc tggggatttg
    74581 agcggcacac catttgccgg ctgatgctga ggccggattg aagccattct gcagcgctat
    74641 tatactcccg ccggacaata tgataccgag ctttgagggc tgtcagcctg ccgtatcgat
    74701 aaatatccat aagaaaatca agatattcct tcggcgtcaa agtaaacagc gctgattcat
    74761 ccggatgaac cgccgatacc ggataactgt ttataggaga gtggctgtcc agagctggcc
    74821 actcaatatt tctgcagatg cttgctttct ggagcagttg aagtctatca cgacagcgtt
    74881 caagctcagt ggctacggct tttatgtcca gttgctcagc cggcagacta atctgctgtt
    74941 ctatcaattc gaagtcggga tttttttccg gttgattcgg gtcttctgtg aatacctgca
    75001 aataaatcgg cagcgaagct tcatttgtca gatcggtctt taacggcagc agatgaaaca
    75061 ttggccggcc gatgtccgcc gctggcgtca gttccacaat cttcatcgcc gaagcagaaa
    75121 tccattgcag aatgtttggg tctgcaggtc cccttgcagg taatatattg ggcttcggtt
    75181 ctgcctgatt gggggcatcg gccaaactgc agacacagac ggttaaaatc aacaataaaa
    75241 aaaacgcttt actcatatat cagatgaacc tcatttctgt ttcgatttac actccgcaga
    75301 cacggcggat ttcatcttcg gtggtaatgc cgcggctgac cagcctccgc ccatcgtgca
    75361 tcagattcat atgaccgcgg gaagccagaa tggcatccag cgtttccata tcgctgcggt
    75421 cgagaatcgc cttgcgcagc atcccgtcaa gctctaccat ctctgcaatc agtacccgcc
    75481 catgatagcc tgtcatatga caccgagagc agccgaccgc ttcgtaagct ccatcaggca
    75541 actgcctttt gcagctgtcg catagttttc taaccagacg ctggttaaga accgcatgta
    75601 tacccgatgt aacctgatac ggttcaatgc ccatctccag cagacgcagc agcgcaccgc
    75661 tgcagctgcc gctgtgcatc gttgtcatca gcaggtgtcc ggtcaatccg gcttctactg
    75721 caattctggc cgtttcagca tcccgaatct cgcctatcat cagcacttgc gggtcttgac
    75781 gcagcagtga ccgcagcgcc atgggaaagg acatctgacc ctgcggctct atttgaacct
    75841 gagtaatccc ttcaatggcc cgttcgacag gatcttccaa tgtcacgatg ctgttgccgg
    75901 gaaattgctg caaaatatag cgcagcaatg cggccaaggt tgtactcttg ccgcttccag
    75961 ccggtccagt cagcagaaga ataccctgat ttctgcaggc aaacttccgc aaagcggatt
    76021 cgatgaagtc attaaggcca agttcatcca gctgcatcag gccgcgattt tcataaaaga
    76081 ttcgaaccac tgcccgctgc ccataaatcg tcgggaaaac tgccagccgc aagtctgtaa
    76141 tagtcccggc ttccgacgcc acagagatat ccttgaatcc ctccatccgg ccttcctgag
    76201 ggatatcgtt gcggtaggtc agaagtccgg ccaagacttt taatcgggct atgatatttt
    76261 cactgattgc ctttggcagc gtttccaccg tattaagcga cccgtcaagt ctgaatttaa
    76321 ccagcaatga cttggcagtc ggttcaaagt ggacatcgct tgccccatat cgaatggctt
    76381 ccgagagaag ttgttcaact aatgctgcaa cgttaggttg tgatgtctga ttcataaatt
    76441 tttttccaaa aatatattgg gctttcacag aaacggacga ccagcaagtc ccttttgtta
    76501 cctaaaaaga gcaaaaaaat ccaactccca taaacaaaaa ggcctgtcgt caaaagaatc
    76561 aaggacaggc cttttacgaa aaatcagact aatttaaagt taaggagccc gatacataag
    76621 cttgaatttc gcaccatttt tcggataaag ataacgtacc gttgaggtgg cttgcaccca
    76681 ttgaaaatca gcaccaacag aagtctctaa acccacatcc aagtcgcagt tataagtgcc
    76741 tcgaacaatc aggccggttc cggaactata cactgttcca tcagcattat aggttaccca
    76801 tccaagggtc agcttgtggt tctctgaagg agtcagctgc tcgacgataa acttgccata
    76861 tcgccctgcc gaagttttgt atacaaaata ggtgccggga ttgaactcca taccatcgga
    76921 gccgtaaata ctggcggtgc tcatctggct ttctaatgtt actacggcgg cccggtttag
    76981 ggcgtcataa tcatgatata cggcacgcca aaaacgtgaa ataacgctga aatcttccag
    77041 cttaacccgg ctgttccagt tgatgtcgct gttttcacac caattgggca ttatacagtc
    77101 ggacaccatc caattatcgg ccacaacaga caaatcgcca agatttactg aacggtcatc
    77161 atttatatca cccggatacc gttcaacata ctgacctgtc aattgcagat taaagtcccc
    77221 cgggtgcgta ttccaattgg accatagttc catattggcc ggctggccgg agacctccgc
    77281 agcataccct gtgctggacg gccatggatt atcccatcgg ccgcagactc cagaggtaaa
    77341 ctcattgtat tcaccctgat agagatagca ggtgaagata tatgtctggt tcggttccag
    77401 ccaaagattg gaaaaaggca tcatcaccca gccgccggta aagtttgacg gaaccagctt
    77461 gacgcttggg gcgagtatgc tgccatctgc tttccgtgca tacataatca gataaaccgc
    77521 gtggccatgt ccttccgggt ttgtgtaata atcaaacgga tcatcaaacc ggaaacctgc
    77581 tgaggtaacg ttggctggac gggttaatgt gatactctgc ccaacgccga cgttgcgttt
    77641 gtcgacccga tcatctccgc caaaccagat aaccgcattg tgctggtcat gaatcacatt
    77701 gaaatccagc agaactccaa tcatcgccca cgtggtcaaa ggaccataga tattgttggt
    77761 ttcataggtt tcttcaactt cgccttcggt atccacctgt gcatacattc ttttaattcc
    77821 ttcggtggca taggtatagg ttaccatcat tgcatctgag gcacctgctg ccaaactgga
    77881 gaatcttttc catcgatccc ctacggtacc cggactgggc aaagaagctc ggtcggcata
    77941 ccagtccaga taaaaagacc ccgcattggt agtacccgca ttatgaatag ttacggttac
    78001 agcaaccggc tggccaacat gcggaacttt gggattgagg ctaatagagg taatccgcaa
    78061 atcgacgtta atatactcaa attcatacgc tcccatatcg atagcggcta tactgtcccc
    78121 attgccgtcc agcggacggg gtttaccatc caaatcctcg gccggagcat caaaatcgga
    78181 cccggcatca atgcaggggc tcgaggcagt caaatggtaa tcaccgctgc ctgtaaacag
    78241 aggattagca tttatattcc ccgtgccggc aaaaccgccc tgaatgtcgc agtaggaggc
    78301 agtagggccc ggactgctgt aataatcgac aatctgattg ggactgttgc cccagaaaat
    78361 gcaatttctc aaggtgggac tggatgtatt ggtataaagt gcccccccgc ctgctgcata
    78421 cgctgccgta ttatcagcaa aagtgcagtt gataatgctg ggccaggcct gatgatagga
    78481 aatcgctcct ccataggatg ctgaactgtt gctgacaaac aagcaattga taatcgagcc
    78541 gtatgtttga taatctccta agatcgcccc gcctccaaag ccgcctgctg tagaggagtt
    78601 tgacttgaaa atgcactttt caatccggga atttgtctgc cactgccaaa tggctccgcc
    78661 gttatcagca ctgttgtcct gaaacagaca ctctttaatc accagattgg aacggagatc
    78721 atacacggca ccgccgacaa cagtggcaac attgttgaga aatctgcagt tttgcaatgt
    78781 tgtgctgaaa tcaaacccca gacagctgta ggttgggctt tcaatataca gcgccccgcc
    78841 gctcgaagca cggccgttct gaaatataaa accgtcaatg atgctgtagg aagtaatctt
    78901 catgcaccgt acagaattgc taccgttgat aattgtcgga taaacggcgg gattgcgatc
    78961 atcaagtgtc ggtgatgcca tagcatttgg aaaaccgcca tacatcacaa caactttgtt
    79021 cagtgtcaat tcgctggtta ggttgtacgt tccgctcttt acccagatct ggtcactggg
    79081 agcatagcaa atcatataca gcgggcttgc agcagcgatg gcagcagaaa tcgaattgaa
    79141 agctgttggc cagcttttgc catctcctcc gggcgcaaca tccgaatcaa cataccaaac
    79201 ggcagcagag agactccctg ctaaatagaa aaagaaaaga aacaatactg atttgccttt
    79261 catctttata ccctcctaaa aatgtatgcc attgaatatt tattctgaca tctttttagg
    79321 ctttgtcaaa tacaaaaaaa gaaatgcaaa aagaaaataa agattaaaca atcataaaac
    79381 tggccttgta ttcagccttt attatgtctg ttatttacag aattattttt tgtcttttta
    79441 tccaaaatct ggtaacatat aggaatgtaa aagcgtcttt gattccaaaa tgagcgtgga
    79501 cgcagaagaa gggataatgg ccatgaatcc agagaccttg cggctggcat ttgagctgac
    79561 gggtagtacg gctgcagaaa aaagccagat gtggatatta tctgcaatgc agtactatgg
    79621 accagctatc gtcagcatgc tgtggcggat tctgggcaga gaagaagatg tttgcgatgc
    79681 ctatcaggaa acttttcttc atctggccta tctgcctcag cagcaaaaac cagccaacct
    79741 tcgtgcctat ctgttccgta cggcttccaa tgtggccata tccatgctcc gccggaggca
    79801 gattgaaaag aaatatcagg agcagattca gcacaaagca attccgtctt ctcctgatcc
    79861 tgccggtttt ttggatgcaa tggatcttca gcagcagctt cgggaagcca ttgctcatct
    79921 tccggactat ttgtccgatg ttgttgtgct gcgggacttg gccgaactgc catatccgca
    79981 ggtggctaaa attcttggaa taacaacgac agccgccaga gtttaccgcc acaaagcagt
    80041 caaactgctg gctcagtgga tgtcaaaatt agataagcgg gacgacttat gaagacaata
    80101 agattaaaca atctgtgcag agttttacag cagcgcaaag tcggtctgtt tgattcgttc
    80161 agacagcacg tgcagcatcg cttaattgaa catatggcaa attgtccgcg gtgccgtaaa
    80221 cgccttggtc tggccagtcg gttagaaatc gctttggcta tgattaaatc ccagccccat
    80281 catattgatc ttttacaacg ggctaacacg caaacattag ctgtatttgc gcatgcactt
    80341 cggtatgctc cgaaatcaca aacattgcgc atcagccggc cagagccaat ttgggcagtt
    80401 aaaaacaggt cccttattga tcgtctgctg aatattgcag cgtgtctttt tgttgttctg
    80461 atgatgcgtt cgggaatttt tcattcgctg accagttata aagaacaaag tgaagccgtt
    80521 attcacaact attacgcccg caatcttgac agcaatatgt tcaatgaaat cttccccaat
    80581 aacgacacgc tggcgtgact gaaatgggca catttcttta tctttgcaga cagaaagaag
    80641 cggctgaaaa actgcaaaag taaaatatat aaattttgtc gagtttttca tcgtttctaa
    80701 cttcttaaat tcctcagcat tagattccaa catatatatt gtcaggcatt ttttctaaac
    80761 ttcgatttat ttatttcttg aaattttgcc gatagtgtga tatagtaaaa tacgcgggtt
    80821 aacccctgcg gtgttagtgt cagaggccta tatttggaag aaccgatgac atccccaagg
    80881 gaagccaggt cgggagtgga tcgggtatgc ctgcacgaca ggacattcgg acaaccgcaa
    80941 cggctgccca cattagaata aggggtttct ttccacatgc ctgctgcacg gcatacgcgg
    81001 aggtgtacct cacataaaaa aggcctgtcc tcgttacagg ccttttttat gcgttcagcc
    81061 aactgctgca attgtaataa actctgctct actgcctctg ttctaattgt ttcactatta
    81121 ttttcaaggc aggattgcca acgggaagct gacggctcag attccagttt tccaaaccag
    81181 cttccagcga acgcaaaaaa ccataatcca tatccccgca tcgtgttgca acaggctcgt
    81241 ctctttcatc ccagcgctgg tatcttcctg taattgtcgc catataccag cgatagggaa
    81301 attcgtgttc gacaatcaaa ggatcaagag cagaaatatc gatattatgt gcttctttca
    81361 gattatgttc agttactttc ctagttatat cacgcgtata atagtgttcg ccccagtact
    81421 gacttttctg acattcaggc aattcgaaaa cttgccccaa ttgttcggct tcccatttat
    81481 ccggtatata ggcatctaaa aatgtcaggt gaaattgtgt gttcggatat tttcgggcaa
    81541 tctgtctggc tgcagaatga ataacccacg agccggcgga atgtccaaca agatgaatgt
    81601 gcttaaacgg tctatccagc ttcagcacag ccgctgcgag tcgggggccg gcgatgtccc
    81661 gggcatactg cgccgcctgt accgatgtaa agaccactga gccgcccttc caatcatacg
    81721 aggcacaaac ccactcattc ggatctatcc gagcggcgat tgcctcggcc atctggcttg
    81781 gccagtcatc ctcgccctta tccagccagc catgcgttac cactgtcaat ttgtctgctg
    81841 tcggaataat gcataatcca tcctgaatta ccacattcgg ctcggcagaa tgtgccggcg
    81901 tcagcagctc atgctgaaca gcaaacaccc cttgatctgc tatcaacaaa agtccggggg
    81961 cctttggggt aatcgttaca ggggctttga gatatttttc cagcccggca ttttccatca
    82021 gttttatgac tttttggcgg gcgctaaaat ccccgcggac gaaggtacag agacataaga
    82081 ataaaatgat cctgctatgc tttgctgtca cacatcggct cctgccttat tttttctgct
    82141 tcatcacagt acgcgcccgc aagccgtctt cttcaaacgt aattcccaat cgaacctgct
    82201 ggactgcttg catgggggtt aataaatcat ccggcattct cggctgtccc ggatgtccaa
    82261 aaactgtcga ttcaaatgta tgaaattgtt cattccaaac atattgaccc ccgccggggc
    82321 aagtcagcct tgtatgccaa taccgcaagt ggaaatcggt ttcactcaat tctcctttcc
    82381 gcattcgcca ttcggccaga atcggcagat gtctccacga ctgcatctgg agatagcggt
    82441 tccagctttg tgatgcaatt atttcaatca ccttccatgc cttttcatca gcctgcacag
    82501 caagattttt gccaagccac tggaccggcg ctttggtgcc attttggcgg gctgatacac
    82561 tggcacgctc aatcgcctgc tgaagaagct tttcatgggg tgttataatc agctggtttg
    82621 gcagtgctgc ataatacaat gacggcggat tctgctttgc ttgctcgtac tgaaaggtaa
    82681 tcttgacata agatttctgc tgccagttca gtgtttccca cacagtcata ttcggtgcag
    82741 tctgctcgat aaaagcacgc aagccgacaa gaaagatggt cagctgggat gggctgtcaa
    82801 catccattgc agccgcaagc ggcagtcgat tgatattttg ttccagatac tcaaaaaatt
    82861 cgtaatccaa ggtatggccg gaaaggatgc cggccagttc tccccaaaaa gaatcttctt
    82921 cggcgtatat tgtaatccat cgccccagcc aactaagcgg atgggtgttt atccattccg
    82981 gtccgcccat cggtcgggca aagccccccg ccaagcgaac cggttcggag tctgaatcga
    83041 tagctaaaat tgcctgcaga attgtttttg cgtgaggatc tccatcatca ggttgaagaa
    83101 aattcttgcc gccaatctgc atcagctggc gatattcact gtctgcaatc agcggacgga
    83161 tagttatgtc aatatctatt gtatcggact ccagcagaac tgcagcagca acaggatcaa
    83221 aaaaagtgcg ccagcgagac tgataactgc tccgaaagcg gttatactga ttgcgttccc
    83281 tttctgtagc tttttcaata gccagctcgc tgatgggtgt cataaatcga agatttccat
    83341 aaacagaaga ggtgattcct gcattggtaa tagtaatatc ccctatatct ggcgcccaat
    83401 tcagcgctgt cgtctgcgca gcggcaaggt caaatctatc ttgattatcc agatagccgg
    83461 cctgaagctg tgataaaact gtcgaaacca gcattctgcg agctgcccct atgcgccagc
    83521 gaggactgca ccagcggcga atagctgcat cagtcaaaac cagaaatgct gtttgcctgt
    83581 tttcatccgc cggataacag ctgcggaaaa aggcatattc acccgcttca gccaaagagg
    83641 gctgcttgcc ttgggctgtc tttatgattt tttccagctg aaccagcgaa ttgctcacaa
    83701 taactgcatt atcaaggcga gccaaatatg aactgactgt cctatccgga gaaactaccg
    83761 cctgataagg tattccgctg accattccgg tcaatttctt ggcatcttta actttctcca
    83821 gcgcttcctg ctgtttagat tcggtattgg aatagaccag tcgagctatt ggtgcatcaa
    83881 aaagtacagc cgaatctgtt ccagccttcc aatacagatc agaaccagtc aaggcaacac
    83941 tggctatcgt tttaggaccc caaaaacgcg accagccatc cagccagaca cacatctgcc
    84001 gctcgtacga ttcgatattt tgagtttgaa actcatccgg aagataccac gtccctttaa
    84061 gcctatccaa taaatgcatc atcgacttga atgtcggata aaaaagagca tgctggtcag
    84121 cgggaataaa acgagctaaa gcatcctttt taaatacaat gccctcgttc tgcttgttcc
    84181 aatctattgc cgatgttgta atgccttcaa taccagcaat gtcaactaca cgcggctcat
    84241 tgaatcgggt ctgcagagtc cgatcgagtt gaatattttc cataactgct ttgcggccgg
    84301 taaaaaagag cattatatct tcaaatggat cctgttcggg caacaatctg ccttgctggc
    84361 tcactcctga acactgctgg tcatccagta ttttctgtgc ctgcttggct cgataggcaa
    84421 accattcagt tccagtgaat ccgcaacttt gcagaagggt gtaatggttt ttcatcgctg
    84481 taaaaaaatg ccgttttgcc gaagtttgct cagcattcac cggttcctta gaaatcctga
    84541 atcgtacagc cgacatactc tttaaattcg ctgttggaag gaaaataatg ccggttatcg
    84601 gcagagaatg gctgctttta atcgcaacag atgcattatc cagcaactgc ccaaccgatt
    84661 gaatccgcca aaaatcgggg cggttttgtc caccagactg gataaattca ccaaaatcga
    84721 tatagatttc ttcttgcctg tcgccggccg catagggcaa ataatatttg atttgctgcc
    84781 ttagacccca atcaagcgga gccggcgacg acagcacctt atccggccac tgtccttcaa
    84841 tctcaatctg ccgcagaggc agacagtaat acatttctgt attctgtgag aagacgacag
    84901 aaaccgtaaa gccggcagca aacactgctg caaaaaggta attttgtagc ctcattttaa
    84961 acaccttctt tctggcttac tttagcaagt tccacaattc tgctttgatt ggattcaaaa
    85021 aatccctttt tctttaagaa ctgatacaag aaagaaatcc caaccaaaat cagccctgtc
    85081 gtcagaaaag tagcaattcg gtattctgtt cccaataccc acatatccag attaattttg
    85141 cccagcagga tggcgaaaat tccaaaggaa agatatcgaa tccaagccga cctgacaaca
    85201 aagccgataa caagcaaaaa cgcagcataa aacgcccacg aaacagatat atacatgcga
    85261 gacaggaatt tccagctatc cagcaccggt gcctgcattc taaaatacca atagatttct
    85321 tcagacagca gaatccaaag cagtatcagt gatgccataa caatagccgc cggcaggggt
    85381 ccgcccttcc cccgtgaaca tttgacaaac catgcaccca gcagcattgc tgcggcatag
    85441 aataccgcaa tggcaaacgg aatatttgca aaaagaataa atggcttata atagacttta
    85501 ttgg
//
LOCUS       DAOTBO010000002        70617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_296_length_70617_cov_5.741674, whole genome shotgun sequence.
ACCESSION   DAOTBO010000002 DAOTBO010000000
VERSION     DAOTBO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 70617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70617
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_70617_cov_5.741674"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      218..337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            439..1044
                     /gene="thiF"
                     /locus_tag="PLE88_00340"
     CDS             439..1044
                     /gene="thiF"
                     /locus_tag="PLE88_00340"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOM74947.1"
                     /translation="MALPVSFELKNAVVGIAGLGGLGSNVAAALVRLGVGKLILADFD
                     VVEESNLNRQNYFADQIGMHKVDATLTNLRRINSQIQLQGHKIRLTAETIPEVFKDCQ
                     VIAECFDRADQKQMIVETVLSKIAGVVIVTVSGLAGYGRSNDIITQRISERLILVGDT
                     VSGTGPGVPLTAPRVGIAACHQANAIAEILLNEKCQRKKTG"
     gene            1041..1253
                     /gene="thiS"
                     /locus_tag="PLE88_00345"
     CDS             1041..1253
                     /gene="thiS"
                     /locus_tag="PLE88_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOM74948.1"
                     /translation="MSAKLKINGQNKIFEGGLPETLGKLLDDLAINQATVVAEIDGCI
                     IERKDFARTPLKEGQTIELIRFVGGG"
     gene            1285..2061
                     /locus_tag="PLE88_00350"
     CDS             1285..2061
                     /locus_tag="PLE88_00350"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOM74949.1"
                     /translation="MKPLIIADRQFSSRLFIGTGKYASSRIMAQAIEASGTEMVTVAL
                     RRVNIDDENDNMLAAIDRNRCQLLPNTSGARDAAEAVRLSCLARAASGICWIKLEVTP
                     DPYYLLPDGEETLKAAEVLVKDGFVVLPYINADPVLAKKLQDVGCAAVMPLASPIGSN
                     QGLKTKASIEIIIRQASVPVIVDAGLGAPSHAAESMEMGADAVLVNTAIATSADPVQM
                     AAAFKMAVEAGRIAFETGLPPQYAAANASSPLTGFLRDEL"
     gene            2058..3209
                     /gene="thiH"
                     /locus_tag="PLE88_00355"
     CDS             2058..3209
                     /gene="thiH"
                     /locus_tag="PLE88_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HOM74950.1"
                     /translation="MSRQTTIQTILAEKHLDQNPECWLGTLGNIKTDQVRQELSKPAG
                     FFSFDRLLVLLSPAAEEYLEQMAQQSQRLTVQRFGKTIQLYAPLYLSNVCVNSCRYCG
                     YNRTNAIKRTRLTIEQALADAEVIAGEGFRHLLLVSGEDRAFITTDYLIKLAEHLKRW
                     FSSLSIEIYPMAQKDYESLFLAGIDGVTLYQETYDRRVYAQYHLAGPKADYDYRLQTP
                     DRFASAQMRRIGLGVLLGLADWRLETLALAEHAAYLMNKYWRSQVSFSFPRIRPARCV
                     HAQWPHLVSDKNLVQMMLALRLCFADAGMVLSTRESATFRDHVIGLCITQISAGSKTN
                     PGGYSSAKQAVEQFEIADTRTPAQVAQMICAHGYEPVWKDWDVSFSKSC"
     gene            3579..4667
                     /locus_tag="PLE88_00360"
     CDS             3579..4667
                     /locus_tag="PLE88_00360"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOM74951.1"
                     /translation="MGQKILLKGNEVMAEAAIQAGCRFFAGYPITPQNEVPEYMASRM
                     PEVGGVFIQAESEVAAINMIFGASAAGARCMTSSSSPGISLKQEGISYIAAAELPCVI
                     ANMQRGGPGLGNIRASQGDYFQAVKGGGHGDYRLIVVSAATLQELYTLTMNAFDWADY
                     YRNPVLILGDGILGQMAEPVEILPYRPVLEIPPKDYILTGCKGRNPRVIKTLILDPAE
                     GLVQHNEHLQEKYSRIERELCLYETYQIEDAELIVTGFGITGRIAKAAVRKARQKGLA
                     AGLIRPITLWPFPKKIYQQAAMTAQKVLVVEMNHGQMIEDVKLAMDCAIPTEFLGKGG
                     GWYPDEKAVLEAIESMLESPAASVKGGA"
     gene            4670..5407
                     /locus_tag="PLE88_00365"
     CDS             4670..5407
                     /locus_tag="PLE88_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOM74952.1"
                     /translation="MAVFKRTTVLKEQLTHYCPGCGHGIVHRLVAEVIDELGIREQTI
                     GTAPVGCAVLLYNYFNCDIIECAHGRSPAVATGIKRVHPDRIVFAYQGDGDLAAIGTA
                     ETIHAANRGENITVIFVNNAIYGMTGGQMAPTTMPGQITTTTPQGRGCHKEGPPIKVC
                     ELLAALDGVSYLERTAVSSPRDVMNAKKAIKKAFQNQIEGKGFSLVEVLSMCPTDWKL
                     SPAQSVEFVNEKMKKIFPLGVFKDQQI"
     gene            5404..5973
                     /locus_tag="PLE88_00370"
     CDS             5404..5973
                     /locus_tag="PLE88_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOM74953.1"
                     /translation="MNETKVLVAGTGGQGVVVIGNILARAAVIENKNVVGMVAYGAEM
                     RGGTAYAAIIISNEEIGSPIVDRPHIAIILNQPSLDKFEEDILPGGLALINTAMVAKP
                     VKRLGLQRIEVAATQIAHDLGNVKVANIVAIGALIKHTNLLSMSSVEQGLTDLFTAKN
                     PKLVPLNLKALHAGAEHSVYVPSSVPVDI"
     gene            5996..6481
                     /locus_tag="PLE88_00375"
     CDS             5996..6481
                     /locus_tag="PLE88_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOM74954.1"
                     /translation="MSTKQNISAFWQTAIGAVNSRRRLPIGIRMTPMIDVIFLLLTFF
                     VLTAEFQKPEHTLPFSLRKTGTHAAAVPNETVKIYIKSQQNTCLIDIDNRLEITLDAQ
                     NPQQGLLMLAQNIQQITENFGAVPIELYCQDSVLWDYVVKIYDVLYALGAYSITFRIE
                     E"
     gene            6606..7877
                     /gene="clpX"
                     /locus_tag="PLE88_00380"
     CDS             6606..7877
                     /gene="clpX"
                     /locus_tag="PLE88_00380"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOM74955.1"
                     /translation="MAKSSGQGSRREMCSFCGRTKRQMDAFVEGPGGIFICPDCIDLC
                     YNIVRQERRRSHGAGTLFEEVPKPRQIKEFLDQYVIGQEHAKRSLSVAVHNHYKRLLH
                     ADSDNDDVEIEKSNILLIGPTGSGKTLLARTLARMLHVPFAICDATTVTEAGYVGEDV
                     ENFLLRLLQAADYDIEAAQRGIVYIDEIDKIGKTTQNVSITRDVSGEGVQQALLKMLE
                     GTIANIPPQGGRKHPEQSYIQMDTTQIMFICGGTFVGLDNIIKKRLGKRMIGFDSEHQ
                     AHHDKKADYSEIISQVTPEDIISFGMIPEMVGRLPIITTLTALDEAALVDILTKPKNA
                     LVRQYQRLFEMEDAELKFTDEALDLIAKKAIKRDTGARALRAIMEELMVDLMYRLPEE
                     PKPGKYIITPEVVEGKVNLFEQARKVKKESA"
     gene            8205..11090
                     /gene="purL"
                     /locus_tag="PLE88_00385"
     CDS             8205..11090
                     /gene="purL"
                     /locus_tag="PLE88_00385"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOM74956.1"
                     /translation="MKQWRFEISSKQGFLDVHGRDILDDIRQFNILSVEDVISEKVYL
                     IEADFDQPFAERVARELLTDPVCQEWHIGKSGIPAGPMPATLIEVHLKSGVTDPVAES
                     VMAALKDMGAGAVESVRTARKYVLLGNVTEKDRETIARRILANDCIEEVIYGMDAEPP
                     SPHTKSYELNLIQIPICGLDNEQLVQLSKKMDLFLNLKEMQTIQAYYREIGREPTDIE
                     LETLAQTWSEHCVHKTLKSSVDMDLDGRKLHFDNLLKDTVFKATKELDKKWCISVFSD
                     NAGVVEFDDEYAVCFKVETHNHPSALDPYGGAATGIGGVIRDPMGTGLGAKPIANTDI
                     FCFAYPDMKLENVPKGVLHPRRIMKGVVAGVRDYGNRMGIPTVNGAIYFDDRYIANPL
                     VFCGNVGLIPRDKCFKHPQAGNLIVVVGGRTGRDGIHGATFSSGEMTHEHETIFSHAV
                     QIGNAITEKKMLDTLLQARDAGLYEAITDCGAGGLSSAVGEMGEKLGAEVDLEKVPLK
                     YAGLSYTEIWISEAQERMVIAVKPENIESIMKIFAAEDVEATVIGRFTGDKKLTLRYN
                     GVQVGQLDMQFLHEGICKYSRNAVWKTKSLSEPVLPSQKNFTEDLLKILGSYNVASKE
                     WVIRQYDHEVQGGSVIKPLMGAQNDGPMDAAVIRPRFHHNKGIVISCGMNPCYGDIDP
                     YWMALAGIDEAVRNLICVGANPDRIALLDNYCWGDCSRPEMLGALVRASQACYDAAIA
                     YGAPFISGKDSLNNEFVCDDGSRIVIPYTLLISGIGIVENIQQCITMDVKKPGNLIFI
                     IGLTKNELGGSHYYKIHGHLGANVPQTNLSQAPVIASRIAEAIRRGLVRSCHDCSEGG
                     LAAALAEMAFAGAFGIEADLRGLPLSPDCVTPAAALFSESTARYLVEVEPQHYDAFAK
                     LMLNVPFGQLGEVIEDKKLLIRDTQQKTVIEAELDILKEAWKKPLAW"
     gene            11189..11989
                     /gene="purQ"
                     /locus_tag="PLE88_00390"
     CDS             11189..11989
                     /gene="purQ"
                     /locus_tag="PLE88_00390"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOM74957.1"
                     /translation="MTTVRAIVLRAAGINCDVETEHALQLAGAQTERVHINRLIAAPA
                     MLDDFQILVVPGGFSYGDDVAAGKILANQIVHHLSDILQRFIENGKLVLGICNGFQVL
                     IKTGIVPGLSGAGRLTLEGEGVTLTNNDCGHYLDCWVHLAPATTRCVFIDPNRRLYLP
                     IAHGEGKIVADSIERLQQIRTGGYVAFRYVDADGQTGLYPINPNGSQDSIAALTDSTG
                     RVLGMMPHPERFVRWTQHPHWTRLKQQDELTDGMTIFTNAVNYIKQTF"
     gene            12012..13238
                     /locus_tag="PLE88_00395"
     CDS             12012..13238
                     /locus_tag="PLE88_00395"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOM74958.1"
                     /translation="MIQINENFLKLKAGYLFPEIARRVSAFAKANPKADIIKLGIGDV
                     TEPLPQAVIKAMHKAVDEMATREGFHGYGPEQGYEFLRSTIAENDFLPRGANVSPDEI
                     FISDGAKCDTGNIQEIFGLDNVVAVGDPVYPDYVDTNVMAGRTGENDGTGRYAKIVYM
                     PCTPENNFVPRLPEEPVDMIYLCYPNNPTGAVATKEQLKQWVDYAVAHKAVILFDAAY
                     EAYIRDDSIPHSIYEIEGARQVAVEFRSFSKTAGFTGLRCAFTVVPKELVAYTKSGRP
                     QPLHPVWNRRHCTKFNGVSYVTQRAAEAVYSPEGKNQVKAIINLYMSNVDTMCQSLQK
                     FGYCVYGGVHAPYIWLQTPRGFDSWSFFDTLLHQAHVVCTPGAGFGAAGEGFVRLSAF
                     NHPDRIKEAMERIGKL"
     gene            13248..14336
                     /gene="folK"
                     /locus_tag="PLE88_00400"
     CDS             13248..14336
                     /gene="folK"
                     /locus_tag="PLE88_00400"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOM74959.1"
                     /translation="MTEQTAYIGLGSNLPDCRVNLCKAIGLLGRHGKVSAVKASSFYQ
                     TKPLGPVNQQDFLNAVVQVKTSMDAKELFDYLRDIEQQMGRQRQQPWGPRTIDLDLLL
                     FENQTINEPDLKVPHPQMHLRSFVLDGLCQIAPAVEHPVLKRSMSELANRLNGRSFRL
                     DSQKAQLVSVAGTIGVGKTTLAAGLAKRLGAEFIAEQYDDNPYLADVYAGHQELALDS
                     ELFFLSSSVSQLSKYKLTAGNWYVSDYVFEKGILYASSWLKSKDLAVYKEHYCAVSST
                     VANPVLVIVMQDTAQNCLERIRRRNRPYEQQIEPVFLEHLSRGFVRFYNQYSGCPVIR
                     ISPDACWTDQQIDCLAEEVRWYLAKMEN"
     gene            14337..15200
                     /gene="panC"
                     /locus_tag="PLE88_00405"
     CDS             14337..15200
                     /gene="panC"
                     /locus_tag="PLE88_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOM74960.1"
                     /translation="MEIIHTIEQIRQCVKAARNRNLTVGLVPTMGALHEGHGSLIIAA
                     KKQCGYVVISIFVNPTQFGPSEDYNRYPRTFDADAAYCRNLGVDAVFAPSVEQMYPAE
                     QLVWVHPEKLTDHLCGASRPGHFRGVATVCTKLFNIVQPDIAYFGQKDAQQVVVIRRM
                     VQDLNIPIQICVCPIIREPDGLAMSSRNRYLSPAERRQALCLYNALTACRSRIAAGQR
                     NAKLLIDLMAGIIADSGGRIDYISIVDPHTLEPLEYIEQTALVALAVHIGNTRLIDNL
                     LIDLNNLSGPV"
     gene            15232..15594
                     /locus_tag="PLE88_00410"
     CDS             15232..15594
                     /locus_tag="PLE88_00410"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF003947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOM74961.1"
                     /translation="MLIKALKGKIHRCRVTQTKIDYPGSIGIDAQMLAAAGISPYEEV
                     LLANVTNGERVETYVVPAPAGSRDIIILGAAARLFSPGDIVIIMNFAYYSPEELQNLQ
                     PKVLVCDEHNNYSVLQKS"
     gene            15595..16542
                     /gene="lgt"
                     /locus_tag="PLE88_00415"
     CDS             15595..16542
                     /gene="lgt"
                     /locus_tag="PLE88_00415"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOM74962.1"
                     /translation="MHPELFTIPFLNISVKSYGTMMVIGFLASVWLMRRMMNRMGQNP
                     EWITNIAMYALLWGIAGARVFYVVHHNDVFRSRPLEIFAVWQGGLELLGGVLTAIAFL
                     GFYLRRRKLPIVLYLDVLAVGLMVGLGFGRIGCFLNGCCYGKVTDVPWAVRFPYGSPA
                     FYGQIQPDPSRGRSQPHMELPDSYFQDGYLKPLALLTPEQKQAISDGAVCSLPVHPTQ
                     IYSSINAFLIAGILYALWMKFAESRPGIVMSLMLVFYGITRIFLESLRDDNPFEYNWW
                     AVYKGGTVSQNICIYLILTGIILLIYFITRTPTRLKTHR"
     gene            complement(16539..16910)
                     /locus_tag="PLE88_00420"
     CDS             complement(16539..16910)
                     /locus_tag="PLE88_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74963.1"
                     /translation="MVTEITPEQLQYLKEQLHASDEEIERFKNQPFCSLEVYTIDQRT
                     GETIGYLFDSLRCIAEGRGTCDGKREIMDWKWATGHKSTRIIEKINNDIAIATERILM
                     PDGSYMEESGKMTRKKKTDGR"
     gene            complement(16992..17171)
                     /locus_tag="PLE88_00425"
     CDS             complement(16992..17171)
                     /locus_tag="PLE88_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74964.1"
                     /translation="MIRSILTMSAIVIAFFSISCHRVTKDTPTIVESANYRFNPLPRQ
                     DAWSTWLVGDWELTG"
     gene            complement(17279..18439)
                     /locus_tag="PLE88_00430"
     CDS             complement(17279..18439)
                     /locus_tag="PLE88_00430"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOM74965.1"
                     /translation="MMDTFDFYRNQLAHLEQDGLLRRCVCIDSAQGPTVQMADGSKKT
                     LFCSNNYLNLAEDQRICHAAIEAIQRYGLGASASRLICGTQLPHTELEQAFAAFLDKE
                     AALYFPSGWMANQGVLTALPQKGDIVLLDKFDHASIIDAARACEAIFHTYRRNQLERL
                     EKYLSEKNYKNRFIVTESVFSMDGDTANLAELVRLKKKYNAILIVDEAHSFGCFGSAG
                     AGLCQQEGLLSDIDILIVPLGKAAGVSGAIAAGPETVIDYLINRARSFIYTTAPSCAI
                     AAAALKALDIIQAEPERRKRLHNNAAYLRNQFQQLGLNIGQSTTHIIPVIIGDSRETA
                     AVSRRLYEQGLFVSAIRPPTVPQNTARLRVSVQSAHTEKHLTELIQALEGII"
     gene            18528..19376
                     /locus_tag="PLE88_00435"
     CDS             18528..19376
                     /locus_tag="PLE88_00435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOM74966.1"
                     /translation="MGIYDRDYSRPVRQELYSMGMGFRTLPPVVKWLLIGNFAIFVAA
                     NLLPKAGKQIYLFGAVLPVNAVYMIQLWRVITYQFLHGGVGHLLFNLMVLYFMGPLVE
                     CQWGSKAFLKFYLICGAAGGIVYTLLVLLHVLPAAMMVGASGAIYGVMAALAVMFPQM
                     RVLLWGIIPMTMVRLIILLVIVSMLMIAFGENAGGEAAHLSGLAMGFLYMKVKPWAVR
                     LRMERSKGAWARKIEQDRLFQAEVDRILEKVHRQGIHSLTAQEKQTLQGATRREQEAS
                     VKSSLQ"
     gene            19524..20921
                     /gene="dacB"
                     /locus_tag="PLE88_00440"
     CDS             19524..20921
                     /gene="dacB"
                     /locus_tag="PLE88_00440"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOM74967.1"
                     /translation="MQTLRNKIVCLSGFIGLVVGFAQGDVVQDLIVRHKQTKTVFSIM
                     AVEAKTGAVLYQLHPDKPMIPASNMKLITTSAALHYLGGDYIFKTKVGILGSDLVIVG
                     GGDPLLADPKNDSAPGQAATLLISRILLAVKDSGIRQIDNIIVDTSFFDNNRVHPSWP
                     KEQLNQWYACEVSGLNFHNNCIRLTAARNGKEAALSMIPENSYVHLINQIQVVSSGTS
                     AVGAYRNSVPNRLLVKGKLNQAAGFDVAIENPAGLLASIVKDRLQKEGIPVRGQILQQ
                     YIKQNEAIRYLVIIETPIAEVLKRCNTNSLGLAAECLVKTISAENTQGHINGEWPHGL
                     NLISRYLDSLGIPKEQYVLDDGSGLSRNNRLSAYCLITVLKDMYFHPDSSIFLSSLAV
                     GGLEGTAEKYFQEPPYKGTILGKTGYIAGVRSFSGICRTPAGDILFSILSEGSSSAAR
                     SCINDITKAIYDRKL"
     gene            complement(20931..21929)
                     /locus_tag="PLE88_00445"
     CDS             complement(20931..21929)
                     /locus_tag="PLE88_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOM74968.1"
                     /translation="MVWIGLGFAVFLMIFSGLFSGAEIGVYRLSRFRLRLGVEQKRKA
                     YTILSKAVNDGPMLILSLLLGNNCVNYLITSIVTILLYRKLENQHKAEIYAAILLAPL
                     LFIFCEMIPKNIFYYKADTLLPRLSWGIWYIHRLFCRSGLLWVFKHLSLFFSKILNLS
                     TNTTEAVDKTQRYQVRQIIHETQEEGLLSQTQREMINRLIDIPGVSAASVMVPIEKAD
                     KIPLMMTRQDLLRHLRQSRFTRQLVYDRNPSDIVGYLSIYDVLSSKEDFETVRPYVMP
                     LAEVSRTASIIEAINILRNRRDRIALVVDYSKGTRRAVGLITISDLIEELTGELAV"
     gene            complement(21929..23224)
                     /locus_tag="PLE88_00450"
     CDS             complement(21929..23224)
                     /locus_tag="PLE88_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOM74969.1"
                     /translation="MPTTAVLASFVFDYGLQMIWMAGLLACSAFFSGSETAFFQLPRK
                     TVRQFAHSTLPIERLIAEILKDPNRFLTALLFGNMIVNVLFFAGSSILLLKIGHRYGP
                     AAGTISGIICFMMLVLFGEILPKTLAYANTVRFCLWASPVCYIVLTSLKPILRALDWL
                     VVQPSLRLFVRHQQTPSISINQLKILLESSRRQGLISNDENLLLTEILKFSFLKVRHV
                     MLPRVEMPICSIASQTAAVRDEMIARRIDKMAVYATGVDDIVGMVHLCDTLLNSQQPL
                     ACLVRKVHFVPEQKTVESLIGFFKNTQTDHAVVVDEYGGVSGFVELEDIIEHLLGPME
                     ELPEQNPIEAIGPLQYRLLADLAIYDWSEAFGINLDEGRLTTIGGFVTALLGKIPKTG
                     DKIRFKNMTFTIEQVKNNRIQSVILNLEPLVNSDSMEAH"
     gene            complement(23253..24014)
                     /locus_tag="PLE88_00455"
     CDS             complement(23253..24014)
                     /locus_tag="PLE88_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOM74970.1"
                     /translation="MAHLLENRKSLHHQIAHFENQCLKLEEQLTQLQPLANLGMAWAM
                     TAHELNNLLTPVINYTQLAMQNPQDAALLDKTLEKTLYLTKQAVQILEKVMLLAGAGR
                     QENKKCLLTSLLDDVFGCLGRDFSKDKITVVRHVPEALFIYGDVDALRQVMMNLILNA
                     HQAMREKGGVLTIAADDGAEFIRIEITDTGCGIKPDDLQRLFTPFYTSGKKNGNGLGL
                     AFCRKIIEMHGGCIAADSVLGQGSRFRILLPKQRS"
     gene            24280..24741
                     /locus_tag="PLE88_00460"
     CDS             24280..24741
                     /locus_tag="PLE88_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM74971.1"
                     /translation="MPDDNYRKPRVLVVDDNIQNLELILAYLEDVECETLSAEGGQQA
                     LDIIYNDPPDLVLLDVMMPKISGFEVCKRIKNNPKTSDIPVIMITALNEMGDIERAID
                     SGTDDFLSKPINKWELLTRVKTMLKLKHLTDKLERTLAYLNEIEQNPLQQS"
     gene            24961..26586
                     /locus_tag="PLE88_00465"
     CDS             24961..26586
                     /locus_tag="PLE88_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM74972.1"
                     /translation="MSKLFEIFGKGITISTADIIWHWLGQMLPPADSSSNDVSFVKIL
                     DHLANREIVQAEEIIRSCLIKSPDCVWARMAASAVCLMQDNIAESLQHAQSVYFRQPS
                     NTMALYVMGYCCERLGQPEQALEFYQDCVKFKRHLQLPHQRMAAIYLRDGGIDKAAAE
                     YEQITAEHYEDIQSLVLLGYLYLDSGKIEQAIDALNLAIVSHPDNFMEFHEPDEIETL
                     IQDEMFEQAIERIRWTIEQIGTLPDLVVRMADIYSAWGKEAEALACYEHAVHLQPSSL
                     EAAIKLATHYLRSHRFSLAAEQFNRAADINEEIVDAYMGLAIAQETSDHSEAALETLS
                     LASAILQNSILLYSESACLQFQSVIDETFTDTAGRTIVSANDVIKAYKEQLKYSGSRP
                     DVNYKYGILMIGENNLPLASCLFEKTLDINPMYYRARHKLALCAFMDGHPQKALKILR
                     NNPSAGKTVFEKYYQLSILYSNRKKFSEAIRQLSVLETSDMADLSQTQANLEIILENL
                     GLIDRTMTSWQRLNETSECLVKIKEKKPISNVL"
     gene            complement(26768..27376)
                     /locus_tag="PLE88_00470"
     CDS             complement(26768..27376)
                     /locus_tag="PLE88_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM74973.1"
                     /translation="MIHYNSSNNLLVLANGWGDIATFIVIALFYLAGAIAKALANRAK
                     SQDQPSGRSSALSSPKKDRGRQISEWDRRLAQKKSQKAAVIPQVRFSAQSIAQQKSQT
                     YKPPARESFIGRIVSPIGGQMVGGSGNQYFIKPPASIPTKTSSAVTQEEKKITAILSD
                     KNKIVKPFTVGLWQTMRSPENLRNSVILKEILDKPLAFRDAF"
     gene            complement(27373..28416)
                     /gene="floA"
                     /locus_tag="PLE88_00475"
     CDS             complement(27373..28416)
                     /gene="floA"
                     /locus_tag="PLE88_00475"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOM74974.1"
                     /translation="MEQLSGLLAAGAMDIILVAILVLVGITALIFILIIAAFFKLWLQ
                     AKLSRADVKFSELIGMWLRKVEPRTIVLSRITAVQAGLDISQRELESHYLAGGRVPNV
                     VRALIAANRANIALSWKTATAIDLAGRDILDAVQTSVNPKVIDCPDPRKGKETVDAVA
                     QDGIQLKAKARVTVRANIDRLIGGATEETIIARVGEGIVTTIGSAITHKSVLENPDNI
                     SRSVLAKGLDAGTAFEILSIDIADVDVGENIGAQLQAAQAEADLKRAKAEAEKRRAMA
                     VAREQEMRALVEENRAKVVLAEAEVPKAIAEAFRKGNLGIMDYYRMKNINADTSMRDS
                     IARGTASKPEEEK"
     gene            complement(28438..28926)
                     /locus_tag="PLE88_00480"
     CDS             complement(28438..28926)
                     /locus_tag="PLE88_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOM74975.1"
                     /translation="MFWWLFLAIVLYILCAVLLVAEVFVPSGGLLSICALACAAGGGV
                     IFFRISSTAGWIGVIIAMFLIPGVLVLAYKLLPKTRFGHSVMLTPPNRQQGDAIPDKS
                     LLDKLTGRQGKTVTPLRPVGTCDFDGLRVQCTAECGFIDKNEPVRVLFVEGMRVVVAV
                     NK"
     gene            complement(28926..30467)
                     /locus_tag="PLE88_00485"
     CDS             complement(28926..30467)
                     /locus_tag="PLE88_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOM74976.1"
                     /translation="MREKESIISRWLVFAILAAFACFIGASLLQAQPQEQEKPLKTAA
                     ILTCYEMIDHGLYQSVVRRSEEAVRRGVDYIILDIQTYGGLVKSADDISKYLILDLAK
                     KARTVAYVSTEAISAGAMISVSCQDIIMRQNTTIGCSAPIVMGDTEMGEAEREKAESF
                     VRAAFSRAAQANGYPEALLKAMVTQKLEVWQVKNKKSRTFEYFEKDYLPTDPNLYDLA
                     GKKLVVKEGELLTLTDQKALEYGIARAVVQNEQEMLDFLAKQDGVVFSKDILCLETLW
                     SEELVRWLNSPVVVSILILGILLGIYIELHTPGLGLPSLAALVCLVILVGSKYLTGLA
                     NWIEVAILVLGIILLLLEIFVIPGFGITGIAGIVCILVGFFAMLVKNRPDEIPWPQTE
                     EAWTLFAQGVLGIIGGVAGFLVIAWVLAKYMPKLGFLSGFILVPAIGQSLQANPPQQD
                     SIRLAVGDRGAAVTPLRPAGTARIGGTLYDVVCQAEFIDKASVIEVVQVSGNRIVVKK
                     IEEPS"
     gene            complement(30600..32462)
                     /locus_tag="PLE88_00490"
     CDS             complement(30600..32462)
                     /locus_tag="PLE88_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOM74977.1"
                     /translation="MCGGLGIFLLGMKNMSEGMQAVAGERLRRMIGAVTNNRLLACGV
                     GTLVTCIIQSSSVTTVMVVGMVNASIMNLLQAIGVILGANIGTTITGWILVIKISKYG
                     LPLLGASSFFYLFSKRDSIRFFFMFLMGLGMVFFGLELMTQGFAPIKEMPGFIAWFHR
                     FHPDTYFDVWKCVLVGAALTAIVQSSSATLGITMALALTGTIDYRTAAALILGENIGT
                     TITAVLASLGAATNARRAAYAHVAFNVIGVCWITAIFTPYTNMVSWLTTKTQLRHLKA
                     VVVETNEDNLIKQYAKEYNISEEVLYFTHPDGYRVNLKGDVLPENSSYVMAGEFEKAV
                     QKHTLADLPLYTPAGTKYSLTGPAIALTHSGFNIVNTILFMPFIGLLARFLMWLVPDK
                     KALEKPKLTYLNIRILDAPAIGIQQSYKELIKMAQTCQSMLKSFRSFLSNDEPNRELA
                     SKIFQKENDLDVIQKEIVEFIGEIMTGSISHELMQEASGQLRIADELESISDYIQGLM
                     KLRLKVRDTQQKFSEGGLADLISLHDKVDQYLDLICQGIEREDSSLEFFSEMQTKGLA
                     ITNLMKECRSRYLVRVGEGVTNPLLGLIYTDMLTAYRRIKDHAFNIVEVLVGEK"
     gene            complement(32614..33762)
                     /locus_tag="PLE88_00495"
     CDS             complement(32614..33762)
                     /locus_tag="PLE88_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOM74978.1"
                     /translation="MTESEKKNIFVHCTELEQYPYPQSCPFKTCRAGQVRKMLDSMGL
                     LTGPNKSVAAPQKATFEELSRFHTKEYLQAIQRADKGDYDYEMLYMNLGTGDCPVFAG
                     MYDYAAWAAGATLTAARAIIAGTAAVAFNPSGGYHHAFDDHAGGFCYLNDVVLGCMEL
                     AGAGKKVVFLDIDVHHCDGVQAAFYDRCDVMTISLHQDGRTLFPGTGFTDEIGAGEGR
                     GYAVNLPMPPGTYDELYLKAFHQVVVPLISARQPDVLVLEAGADTLQGDPLAGLCLTN
                     NVFVDIINSLLAFGKPILAVGGGGYNVQNTVRAWALIWTALCGEQLAQDMTAGMGGVM
                     LENMDWAGGAGLRDRVLIPSAWQRQNLEPAVNQIIEQVKQTVFPIHKL"
     gene            33953..35989
                     /gene="fusA"
                     /locus_tag="PLE88_00500"
     CDS             33953..35989
                     /gene="fusA"
                     /locus_tag="PLE88_00500"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOM74979.1"
                     /translation="MANLKDIRNIVLLGHGNAGKTSLVEAILHTAGVTTRLGSVEEKN
                     TVSDYDDEEKQRGNSIHSSLMYINFNGTHINLIDTPGYPDFVSPALVSIPAAETALIV
                     ISASAGIEMNTRKLFAAATSAGKARVIVVNKMDSENADLTELVSQIQESFGQQCRCAN
                     LPSADKTSVIDCIANEAGQSAVMDVAQAHTELLESVIEADDALMESYLGGEAVSPEKI
                     ADVFVKAVMAGTVIPIVFTNARKEIGIKELLTLIANCTPSPADVPPAVLKHGDKTIEV
                     KADPNGPLAGLVFRIGYDPRSNMKYASIRLFSGSLDSGTQLYINDAKKPVRPGHPLKM
                     QGGEVKEVEKGLCGDIITLAKIDELKLGDTVHNGSFEGKFAMPPVPKPMFALALEPVA
                     RGDENKISAALEKLADEDACFKVTRDTQTKELVINGLGDLHLRVMLSKLENRYKVHVN
                     TKPPKIPYRETITGKAEGHYRHKKQTGGAGQFGEVYLRVEPAPRDSDPSLIYSWDIFG
                     GSIPGQFEPAILKGINDVMENGFLAGFPMQDIKVSIYDGKYHPVDSKEEAFRTAGKGA
                     FLDALEKAKPTLLEPIVNIEITVPAENMGDITGDLASRRGRVSGQEMLPGGMMVVKAQ
                     VPLAEVANYNSQLKSVTGGQGSYSMELSHYEPTPPNIQQQVIEQYKKEKQEEKE"
     gene            complement(36155..37624)
                     /gene="gltA"
                     /locus_tag="PLE88_00505"
     CDS             complement(36155..37624)
                     /gene="gltA"
                     /locus_tag="PLE88_00505"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOM74980.1"
                     /translation="MTEKEFQELVEKHKAGLLKPKDRFAIPPQDMPSQDAVRRRSNMN
                     EVALGYSPMQARLEALRCLQCKNAPCIDGCPVRIRIPQFVAAIADGRFEEALSIIKQN
                     SLLPAVCGRVCPQEVQCQEKCTVGLKFKDVSKSVSIGRLERFAADFCSRQDSVPAVAS
                     ATGRKVAVIGSGPGGIVAAADTRRAGHDVTVFEAFHKPGGVMVYGIPEFRLPKAIVQK
                     EIDVLKKMGVQIVCNFIVGRTRTIEQLMTEDGFDAVYIGVGAGLPKFMNIEGEHLIGV
                     YSANEYLTRANLMKAYAFGRGADTPIAVSKNVAVLGGGNVAMDAARMAVRLGAQNVYL
                     VYRRSEKEMPARVEEVHHAKEEGVQFCTLQNPKRILGNSDSHVAGIECLRYELGEPDE
                     SGRRRPVPIKGSEFMLDVDTVIVAIGNEANPLIQQTTPGLEFNKWGNIVVDENCKTSM
                     EGVYAGGDIVLGAATVILAMGQGRIAAAAINRYLAGRQQ"
     gene            complement(37662..38528)
                     /locus_tag="PLE88_00510"
     CDS             complement(37662..38528)
                     /locus_tag="PLE88_00510"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOM74981.1"
                     /translation="MSFKIVSKRQLSENVFQFDVEAPMVARARKAGQFVIVSADTDAG
                     ERIPLTIAGADASRGTIRLIFQRVGKATAQMAQLQQGDSLAYVAGPLGRPTHIDKFGT
                     VVCVGGGIGAAPLLPIAAALKEAGNRLVSIIGARSKELLILEEEFKAVSNELIIVTDD
                     GSKGRKALVTEPLQEVCRREPKPDLAVAIGPAVMMKFCCQTTKEYGIPTQVSLNTIMV
                     DGTGMCGGCRVEVGGQTKFVCVDGPEFDGHQVNFDLMIKRMSAYKSLEQKAYEEFRTH
                     VCKIGLNPKTTP"
     gene            complement(38655..39638)
                     /gene="holA"
                     /locus_tag="PLE88_00515"
     CDS             complement(38655..39638)
                     /gene="holA"
                     /locus_tag="PLE88_00515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOM74982.1"
                     /translation="MSKKTTETSRPFIVLCGDDPYVVSVECQRLLDSLLDQNEREMAL
                     YEPRAEEARIADILEELWTLPFLASKRIVVIKNAESFIRSNLEALEEYIENPSPSSVL
                     ILTATSWDKRTRLHKKLQKLNAVIEIPGIKTYQLPAFVSNYAQQTYGIRLDSQCSRWL
                     VELTGDDPGRLCREIDKLAAYAAPSKTISLKDIEALIGHNRLFDAFDVIDAVSTGRTE
                     DAISRLRRMFAADRDSEYTVIGAFGYHFRRLFRVKALMSRGTPPIQAAKNVDSRRPDD
                     LLAQADRLSLPQLASILAELGRIDYEMKTGRTTASSAMERLIVKIISAQQK"
     gene            complement(39654..42215)
                     /gene="gyrA"
                     /locus_tag="PLE88_00520"
     CDS             complement(39654..42215)
                     /gene="gyrA"
                     /locus_tag="PLE88_00520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOM74983.1"
                     /translation="MDENTVHPEYTEHIEDMQILDELKNSYLNYAMSVIVARALPDVR
                     DGLKPSQRRILVAMNDLNLGPRSKHRKCAKIVGDTSGNYHPHGDQATYGTLVRMGQEW
                     NMRVMLVDPQGNFGSIDADPPAAMRYTEARMTQAAVDMLEDLKLDTVDFVPNYDETRQ
                     EPTVLPAKFPNLLVNGASGIAVGMATNIPPHNVEEVCDALLLMIEDPDCGFKDILERL
                     PGPDFPTGGIICGRKGIMDAYVTGRGHLRVRCKAHVETNKRGKESIIITEIPYMAVKS
                     NIVAKIAECVQNGQIDEISDIRDESDRKGLRIVVELKKDADSNVVMNKLYRFTPLQTT
                     FAVNNVALVNNRPETLNIKQMLRLYINHRLTVIRRRTRHLLRRFRNRAHILEGLILAV
                     SDIDEIIALIKASPDGPTAKINLMKKPLRLSESQTLRKLLPAEFVDLRTKNDHFLSGP
                     QADAILTMQLQRLTGLEIEKLAKEYADVAEKIAGFEALLASRDLQLDVIREDLFEIKE
                     KYKSPRRTQIVMDEADTLEIEDLIADEETLVTITHAGYIKRTEIDAYRKQGRGGKGVI
                     GSDTKEGDFLEHLFVASTHDTLMFFTSKGMCHWLKVYNVPAMSRQSKGRNIINLLQLD
                     KDEKITSIINVREFDDKRQLVMATQNGIVKKTALSAYSNPRSSGVIAIKLDEGDVLIG
                     AAITSGSDDIILGTRNGMAIRFHETDVRPMGRVSRGVRGITLRDEDRVIGMVIAEEGA
                     FLLTVCENGYGKRTELSDYRVQGRGGIGLINIKTTERNGKVVAIKAVHDDDEIMMITA
                     NGMIVRTGLEEVRAIGRNTAGVRMISLKEGDKLVAAERLIVEEDQQDIQDDQSKP"
     gene            complement(42441..43025)
                     /locus_tag="PLE88_00525"
     CDS             complement(42441..43025)
                     /locus_tag="PLE88_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOM74984.1"
                     /translation="MVLTKTVNDTTKDLRLPMLIGPIAPGRYEKFEFACVDQLLVLQW
                     GSRRLTYDLSADPDFSPQPASSETPPRVQIFAAGPVQIRHVGLFRDIFYMGKEGYSLR
                     ATDEIPFVLGREQYFVCGDNTGNSLDGRLWSNEGIGNRGRTYDAGVVPRDYLMGKAIL
                     VYWSQAFSPADNLPPIIPNLDTIKAIFGGSEQLY"
     gene            complement(43016..43903)
                     /gene="lepB"
                     /locus_tag="PLE88_00530"
     CDS             complement(43016..43903)
                     /gene="lepB"
                     /locus_tag="PLE88_00530"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOM74985.1"
                     /translation="MEQNQPYQSLAHRHQHSVENIVTLFEWLLVAFILALLFQGFAVQ
                     AFQIPTGSMADTLRGAHYHLRCQQCGYAFDIGSDTAGFLPPRCPICNCSQPPQTADNM
                     KNGDRIFVLKSIYQFFEPKRWDVVVFKNPINPKDNYIKRLIALPNETVQLINGDVFIN
                     GQIARKPAGVQKELWTPIFLQDNPPLVNNDDPMCPLNKPSPLPFQNEPNSTWQLQRAF
                     FSLEDTSGQLHTINFVPNDPSCFRAGCAYNDGWDKESKPIVSDLMPNMKPAAAVLGLP
                     LKNTMFSTPHASNSGELWF"
     gene            complement(43950..45524)
                     /gene="lepB"
                     /locus_tag="PLE88_00535"
     CDS             complement(43950..45524)
                     /gene="lepB"
                     /locus_tag="PLE88_00535"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOM74986.1"
                     /translation="MSSIFNDNLVFSGKDKIDSAYRNAEWLITAFANTLVFIIFVMQV
                     YRIPTGSMAETLRGAHFRIRCGQCGFRYDHDFIAQRYGMSDTITPSQKLPIGPRSSRC
                     PSCGYAEPAPIRSSDGRYFIYKDGKTVPAFARTVFKGDQIFVIKCIYQFFQPKRWDVI
                     VFKNPLEPRINYIKRCIALPGETLQIIDGDIYINGLIQRKPPNVQEELWMVVYHNDFP
                     PARPDEKSFNGHTWRQPFECVEASQWNLSADGPTVFELKSSDSAIHRIQYNDRQGNDF
                     RATYAYDDPASYPMMPLCSDLMAEYYASIGQNSAAGAQIRKYGIAYEGWIHSDGRMEI
                     ARLSPAGEPVIIAAGQCRPDDLKRITRFRFATLDHQLILEYGSSKVAYDLGNGIEDAG
                     TDYHIRPLVQIFGIGSLRLSHIALYRDIHYISDNIKRGTKESPMVMHDGEYFACGDNS
                     PFSLDSRLWDRPGLANPGRPEYPAGIVPQDYLVGKAFVVHWPGGYRIEKGPLSRIPIR
                     WIPFVDGMKVIYGGTE"
     gene            45793..46110
                     /locus_tag="PLE88_00540"
     CDS             45793..46110
                     /locus_tag="PLE88_00540"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOM74987.1"
                     /translation="MSTITKKDLIDRISDATQAKRVVVKRIIQSFLDEIISELAADNR
                     LEFRDFGVFETRSRTARVAQNPKTLERVKVPSKRSVKFKMGRLLKERLSGSSSLAPTR
                     DAD"
     gene            46173..46406
                     /locus_tag="PLE88_00545"
     CDS             46173..46406
                     /locus_tag="PLE88_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOM74988.1"
                     /translation="MAEGKVKWFDKKKGYGFILSEEGKDIFVHYTSFADSSLRSLNDG
                     DIVVFDIVPGEKGLRAKNVSLKTAAKATADSGL"
     gene            46534..47169
                     /locus_tag="PLE88_00550"
     CDS             46534..47169
                     /locus_tag="PLE88_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOM74989.1"
                     /translation="MSTITYHEALDCRVLVLNKHYLALRVIGARRAFSLLFRNLAEVV
                     SCDDGTYANYNFQSWMEVSEAKRTFEAHKYDWIRTVNFDIAVPRVIRLLCYDRIPNKS
                     VKLNRRNIFARDSCHCQYCGKKFPPAELSLDHVIPRRLGGQTTWENIVCACMKCNVKK
                     GGRTPAQAGMKLVQLPEKPKRNPVIHIHLSHERYSSWKQFLEHAYWSVELT"
     gene            complement(47185..47925)
                     /locus_tag="PLE88_00555"
     CDS             complement(47185..47925)
                     /locus_tag="PLE88_00555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOM74990.1"
                     /translation="MSASPDYFDVNVEPVNYPGPFNSDPSESAVPPLDGREDEISTVR
                     DSVLNEPVFTGQLKQAELGQWLAQKRSQCTRLGNWAVTLAAALTGGSFAVLGAVISGG
                     QGLTHALYMTVFAPIIEELLKQSGMIYLLEKKPYRIFSAGQFLIAAAVSAFIFSASEN
                     LLYIWLYIPISSLNNPHQYSLYRWTVCTLLHLVCSLIASLGLIRVWKIQLKDSRAADL
                     SHGFVFFAAAMALHGLYNLAAGLFHPHF"
     gene            complement(47922..48374)
                     /locus_tag="PLE88_00560"
     CDS             complement(47922..48374)
                     /locus_tag="PLE88_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOM74991.1"
                     /translation="MIELLLQIAIPLILVVVFMLIGKGTELHHFRQLRQQEECYKDMM
                     ITNLKSIPPELENTQPFLVMGAAVIATDYFKVFAAGLRTLFGGEMKSYVTLMERARRQ
                     ATVRMLEQAKQQGARIVWNIRYETSAIQGQQKNKPGGVEMLAYGTALK"
     gene            complement(48371..48730)
                     /locus_tag="PLE88_00565"
     CDS             complement(48371..48730)
                     /locus_tag="PLE88_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOM74992.1"
                     /translation="MIVVNTETIPGCKILQIKGLVQGNTVRAKHIGRDIAASLKNLVG
                     GELTGYTELLVESRREAMARMLAQAQQLGANAVVNVRFATSSITSGAAELYVYGTAVV
                     AQPITPSAAVQQKGEGQ"
     gene            49069..50358
                     /locus_tag="PLE88_00570"
     CDS             49069..50358
                     /locus_tag="PLE88_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOM74993.1"
                     /translation="MVSLTNAPVNLFGAELPSFDQIKKLGQFVNASEAIRLKFRQEAD
                     KQSDVLIQGIALLLCGQAGEAIEKLNKGKECPQKHLALGYAYRKVKLYDKAIEQFDKA
                     AKQTEGMTIAMEKAETWRLAGQLDKCEAELKNCANFENVSAEYHYQLGRLLDARGDYE
                     GAIGQYERAVELDNRHINALFHLAYAFDLRGDDEAAVNYYKALIQIEPAHVNGLLNLA
                     VLYEDMGEYEKAMRCVDTVLRSHPNHSKAQLFRKDIASSSVMIYDEEKEKRRDRQNKI
                     LEIPISDFELSVRSRNCLKKMNIVTLGDLLRTTEQELLSYKNFGETSLMEIKKILEYK
                     GLRLGMSLEEEGPAKPVPAEFTGQASPEILNKSTDELELSVRSRRALERLGVKTVFDL
                     INKTEAELLGCKNFGVTSLNEIKDRLAGLGLSLRKLE"
     gene            50388..51644
                     /locus_tag="PLE88_00575"
     CDS             50388..51644
                     /locus_tag="PLE88_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOM74994.1"
                     /translation="MKGIRRLTAYRSAALWACMVCMLLPAGCRQRSPEPTVGTERPIQ
                     EWMRVLLFGNLRECSVASLSGFRIEDIDGGSSAEFMTSAPIQIRHQDGFIQVGDHFFG
                     RQILIKPNAPYVVFEIDGKAYRGYLRLHIRMDEAGMEVINEVPLESYLFGVVGAEMQS
                     YWEPEALKAQAVVSRTYGLYIKSRFGALRSWDLTQSEANQVYRGIEAETPSVRQAVLE
                     TAGQILIGKWADGSESLFPAYYSSSCGGHTEAAQNVFGGSAAVPLSGVLCPYCKEIAR
                     RKDFHWPNVTLTKRQIFEGLTRRYESLEKLEGIEDFEISRVGFLGRVTQVRLLGKNGK
                     TDTLRGEDFRLSLDPTGRKIKSAVFTVDKNADSVVFRDGLGFGHGVGLCQCGAQGMAR
                     QGYTYQEILDFYFPGSKQVAIETSKE"
     gene            51644..52774
                     /gene="tgt"
                     /locus_tag="PLE88_00580"
     CDS             51644..52774
                     /gene="tgt"
                     /locus_tag="PLE88_00580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOM74995.1"
                     /translation="MNSFCVQNKDLHSQARCGCLTTAHGTVQTPAFMPVGTRGCVKGI
                     TPEQLKATGAEIVLANTYHLLIRPGPDVVEALGDLHGLMGWSGPILTDSGGYQVFSLS
                     TLNRIGEDGVEFASHIDGAKIYLDARIATQAQNRLGADIIMCFDECTPWPCPPERLKK
                     AVQRTIRWARQCKEAHTNSRQMLFGIIQGGVDLELRSLCAAELVPMDFDGYAIGGLSV
                     GEGHDFMVQTVRHTAGLLPEQKPRYLMGVGLPIDIVESVRAGIDMFDCVLPTRNGRNA
                     YAFTDNGPVRLRNSRYVTDTEPLEPGCRCYTCQHFGKGTLRHFFNVGEMLGPILVSIH
                     NLTYYQRLMSRIRSEINNGSFQAWADDFCIKHKNEIDEINES"
     gene            52798..53226
                     /gene="yajC"
                     /locus_tag="PLE88_00585"
     CDS             52798..53226
                     /gene="yajC"
                     /locus_tag="PLE88_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOM74996.1"
                     /translation="MHNIWILASSEPESKEEGQIVTMEESASNQQTATSQDGRNGADQ
                     QSPPMSQKPNTMFLVMMAILLVYMFFVMFRGPKKKQQEHQRMVQSLKKNDRVRTIGGI
                     FGTVMDVRDDEIVLKIDESTNTKMRVSPAAIAAVLKDKTE"
     gene            53269..56952
                     /gene="secD"
                     /locus_tag="PLE88_00590"
     CDS             53269..56952
                     /gene="secD"
                     /locus_tag="PLE88_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOM74997.1"
                     /translation="MDKNLIWKSVLIFVVVALAAWNLYPPQEKLKPGIDLAGGTSLIY
                     EIDTTDLAPGEQRNLAQNMIPILLKRIDPTHVANVVMRPQGDTRIEIQLPAASLDTQK
                     KRAAYEAALTALEEENINLLKIRQALALPSEQRQQAFEQYAKGNDVRMQILTDLASIY
                     DQRKHTQQQRDQLIGEMDNVKTKFSAAGLDYETLQYDLRVWSKEEAAKQKESIETYVK
                     NNIKDETAAEGTLGQRQKEAVSLINEYLELYSRWVPVADALTRPETGLNVQWDNAVKK
                     LAQLNLSIELFQDVLEMPKDSRVRREKLEEMKKAYPERSSKIEAAVAAFDAYRVVGGR
                     LDDPEDLKRMLKGSGVLEFRILPTSEEGNSAEFQAYLEALTTKGPKQASDNRYIWCAI
                     EDPQTWPAGLGITGTFGEKKYVLCSNLPNEKMLHEGEKKWKLKKAYPTFDEQGRRAIG
                     FTFDEIAANRFYAVTSSNNDRPLAILLDDQVISAPRINSAIRSSGIITGSFSQTEVSD
                     MVNKLNAGSFPARLSEVPISEKSISATIGTDNRDRGIRAGMIGLAAVAVFMLGYYLLS
                     GAIADAALVLNILLVLGVMAMLQSTFTMPGIAGLLLTIGMSVDANVLIFERIREEQQR
                     GSSVKAAIANGYSRTFRTIFDANVTTFGVAAILYMAASEEIKGFAIVLMLGILASMFT
                     ALFVTRVIFDWLTSRRILTRPLMMFRMFQNANINWMRLRPVFFVISGILVISGLLVFF
                     LRNDSTNSKYDIEFIGGTSVEIELKEGTGFNREKVEQIFKAYANSIGNSALTAAKIYE
                     VGKSGRQFEISTTETNKTTATIVLNSAGTETAAALAEKIRKASAETGKLLSNLNVVSI
                     GPNTFKVSTSRLNAALVQEVLSAAVGADGTVTNVEVQEVVSDAVREAFKDYLTVRENL
                     GLSFIDEQKIETSLDDAALLSDYLGGIKLTCQLQMEATAAELEARISTLLSKPDMQEL
                     VRYPHKLLKTDLTEPAADEPLKSFVYVSVHPEAGYRELDQSEWQNFVNNEKTKLAQAA
                     SLETSLSRVTQIDPSIGSQAKQRAIVAILLSLLAIVGYIWVRFGTARYGMAAIIALVH
                     DVIITLGLVTACTYIAPTAFGKALLIGDFKINLEMIAAFLTIIGYSLNDTIVVFDRIR
                     ENRGKLNILTPSLISSSINQTLSRTILTSFTTFLVVLVMYIWGGEGLRGFTFAMMAGL
                     IVGTYSSIAIASPILLMGKSQEQ"
     gene            complement(57010..58230)
                     /locus_tag="PLE88_00595"
     CDS             complement(57010..58230)
                     /locus_tag="PLE88_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOM74998.1"
                     /translation="MKPKTFLQQWRFTIVLTGAVLIGSVIGVVFGSKARLLKPFGDLF
                     LNLLFCAAVPLVFFSIASAVASSANIRRLGQIAGAMLLVFALTGIISSCLMLTAVKLY
                     DPGQGITLQTTQTNTTSSADWAEKFVETFTVPDFTALLSRQNMLALIVFSLFVGLASQ
                     TAGQKGRVFQDFLCSGAAVMGKVISLIMLYAPIGLGAYFAYLVGALKPQLADIYIRAM
                     MLYYPLAFLYFGLGFSVYVLWAGGVSSLRQFWTHIPPVALTAWGTGSSLAALPVNMEA
                     ARRIGIPEEIREIILPLGAIIHMDGTCLAAVVKIAVLFSIYGHPFAGIETYLTAIGVS
                     LLCGVVMSGIPGGGFLGEVLIVSFYGFGAEALPIISIVGTLVDPPATMVNASGDTVAA
                     IIVSSWLGRKTPRN"
     gene            complement(58256..58329)
                     /locus_tag="PLE88_00600"
     tRNA            complement(58256..58329)
                     /locus_tag="PLE88_00600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58293..58295),aa:Arg,seq:cct)
     gene            complement(58389..59594)
                     /locus_tag="PLE88_00605"
     CDS             complement(58389..59594)
                     /locus_tag="PLE88_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOM74999.1"
                     /translation="MINSPIDSKSAGQRNEHLILSMLRKHGRLSQSELCRLAGIRSST
                     ASTIVARLREKGFVTERRGSSSKRGPKPILVELNPDYLYLIGIEINPSYLVLGLFNFV
                     GFLKNKIRIPLGEDHSIGHVLTLLKDRLPDLLDDHRIDGSQIMGAGVTLSGSVTPDGN
                     ISLSSPMGWKNINLKEKLSAVLPFPVSVFSNRVRLLAEFEVQPELASRNILYINAANG
                     VGSTLYMNGQLVFGASGRYGEIGHIIADPAGPLCGCGHQGCLEAIISGPALAAKIKQD
                     IQHGTKTAFANSISHTPEELLADWPVHIERGDRYALQLREYVSRYISWAAAILINCYD
                     PDIIILAGYVVLPFLPFLAQKIQQQIQTSVYDNALREIPIIPAQAGEDALIKGVAAAV
                     IRQTHSPIW"
     gene            59810..60628
                     /locus_tag="PLE88_00610"
     CDS             59810..60628
                     /locus_tag="PLE88_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM75000.1"
                     /translation="MTNLFSVKDKCIVITGGAGILCGALAEDLARSGAKVCIADYDEF
                     RANQLAEKINAAGGFALAVRINVLEKKEVEEAFACALESLGRIDALINGAGGNKKEAT
                     TSDQLAFFDIPADAFRWVFDLNCLGTILPCQVFGRYMAKQKEGVILNISSMNAFRPLT
                     KISAYSAAKAAVSNFTQWLAVHMCQNYSTSIRVNAIAPGFFLTEQNRFLLTDEKTGEL
                     TPRGRTIIGHTPMGRFGQPEELCGTVRWLLSDASKFVTGTVIPVDGGFSAFSGV"
     gene            60652..61560
                     /locus_tag="PLE88_00615"
     CDS             60652..61560
                     /locus_tag="PLE88_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOM75001.1"
                     /translation="MANPAQALIDMKPTKPFLVAIDSDGCAFDTMGIKHRECFCPMLI
                     AYFGLQPVAQAARECKDFADLFSKTRGANRHKTTIRILQELLPTHPMVKERGFAVPQF
                     PHYAAWVNNPKSLLSNEGLKKAIDETQNEEAKKELQTALTWSKRVNELVAEIVKNIPP
                     FPYVRECLEKIYPAADIIVCSATPTEALHREWAEHNLARYAKVIAGQEMGSKAEHLSI
                     MRQKYAPDKILMVGDALGDQQAAEKNGVLFYAINPGHEPASWKRFYDEAFDKFVLGTY
                     AGDYERRIKIEFDGYLPQLPPWLSKG"
     gene            61564..62829
                     /locus_tag="PLE88_00620"
     CDS             61564..62829
                     /locus_tag="PLE88_00620"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOM75002.1"
                     /translation="MLYFARGNENAFLSHHDLQEGLITALEALGPRRKVLAIPPDITR
                     FFSRAGELTRYAADYYGKKLTDILPALGTHAPMTEEEIRRMYADVPLELFRQHNWRKD
                     LKTLGRVPAEYIREISEGRVDYDWPAQVNQLLTDGGFDLILSIGQVVPHEVIGMANYN
                     KNIFVGTGGAEGINKSHFLGAAYGMERIMGRADTPVRRVLNYASEHFAGNLPIVYVLT
                     VVSAVDNRQAVRGLFIGDDIECFNLAAALSLKVNFKMLDKPLKKAVVYLDPHEFKSTW
                     LGNKSIYRTRMAMADGGKLIVLAPGLKEFGEDKEIDRLIRQYGYVGTDRVLQLVEANA
                     DLQNNLSAAAHLIHGSSEGRFDVIYCPGHITKEEIESAGFSYAELDAMMHKYNPCLLK
                     DGFNEVDGEEIFYISNPGLGLWAYRERFK"
     gene            62864..64315
                     /gene="gnd"
                     /locus_tag="PLE88_00625"
     CDS             62864..64315
                     /gene="gnd"
                     /locus_tag="PLE88_00625"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOM75003.1"
                     /translation="MAKADIAVVGLAVMGENLILNMESKGFTVACYNRTVSKVDDFIN
                     GRAKGKNIIGCRSIEEMTAALKRPRKVMIMVKAGKPVDDFIEQLLPHLGDGDIIIDGG
                     NSHFPDTIRRTQYVESKGKLYIGTGVSGGEEGALRGPSIMPGGSPAAWPHVKPIFQKI
                     AAKTDKGEPCCEWVGENGAGHFVKMVHNGIEYGDMQMICETYQMMKQGLGMSNAQMHS
                     VFAEWNKGELDSYLIEITRDILGYKDSDGNEVVDLILDAAGQKGTGKWTVLAALDSGQ
                     PLTLIAEAVFARCLSALKEERVAASKVLAGPNKQFNGNKNDFVNDLRQALYASKIVSY
                     AQGYQLMRAAAKEYNWNLNYGGIALMWRGGCIIRSAFLGKIKEAFDKNPNLSNLLLDP
                     FFKEAVVKAQDAWRRVVTSAVTIGIPMPAISSALAYYDGYRSERLPANLLQAQRDYFG
                     AHTYERVDKPRGQFFHTNWTGRGGDTAASTYVV"
     gene            64352..65764
                     /gene="uxaC"
                     /locus_tag="PLE88_00630"
     CDS             64352..65764
                     /gene="uxaC"
                     /locus_tag="PLE88_00630"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOM75004.1"
                     /translation="MKTPYMHEDFLLQNDTAKELYHNHAEKMPIYDYHCHLPPAQIAE
                     DKRFANLTQIWLYGDHYKWRAMRTNGIDERYCTGDADDRQKFRHWAQTVPFCIRNPLY
                     HWTHLELKKPFGITDMLLSAETADTIYDRCSEMLQSPEFSVRNIMRKWNVRLVCTTED
                     PLDMLEHHKAIRKDGFEIAVHTAWRPDKAMMPENLDVQNAWIDKLAELTNIEINTFDR
                     YLEAIQQRHNYFHEHGCRLSDHGLDRPFAADYTDKKIQAIFLKIRSRSPLSPLEIDQF
                     KSAMMIEFGKMDYRRGWVQQLHIGALRNNNSRMFAKLGPDTGFDSIADYEIALPLSKY
                     LDKLASQDMLTKTILYNLNPKDNEVIATMLGNFQGDGIPGKIQFGSGWWFLDQMDGMT
                     KQMNALSNLGLLSRFIGMLTDSRSFLSYSRHEYFRRILCNLLGEEVEKGFWPKDMTLL
                     GRMVEDICYNNAVNYFSMKL"
     gene            65847..66092
                     /locus_tag="PLE88_00635"
     CDS             65847..66092
                     /locus_tag="PLE88_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOM75005.1"
                     /translation="MTQNLLAIFGLPGGGEWIIILIIAVLIFGRRLPEIARGLGKSIT
                     EFKKGIKDTEDEVKDALEESDKIAEKHTEKKINPPSD"
     gene            complement(66108..66884)
                     /gene="tatC"
                     /locus_tag="PLE88_00640"
     CDS             complement(66108..66884)
                     /gene="tatC"
                     /locus_tag="PLE88_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOM75006.1"
                     /translation="MSESKRRHHDEEELKSMSLGDHLEELRARLILSLVGLAAASIIG
                     LVFGKWFLQFILWPYKRAMTELNIEMKLLAIDVPEPFMVYLKASLVLGILICSPWLFY
                     QIWAFISAGLYTHERRFVKMVAPASAFLFTTGVLFFLTVIAPFVFKFFVRFNPGIDYL
                     NYQPSLTKSVNFILILSLVFGAAFQTPIAIVFAERMGLVSVETLCRVRKYVFLGCFVV
                     GAIATPPDVVSQISLAVPLYFLYESSIIVCRIWRRKRQVK"
     gene            67364..67774
                     /locus_tag="PLE88_00645"
     CDS             67364..67774
                     /locus_tag="PLE88_00645"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOM75007.1"
                     /translation="MEQNAENQPTSSENQPQPSSQSPQSSNISQVPQDSRNMAMLCHL
                     LGLFTSFVGPLILWLIKKDEDKFVDSQGKEALNFQITVIIAGIAAGLLSFICIGIFVA
                     IAVSICDIVFCILACVAASKGQDYRYPLSLRLIK"
     gene            67905..68375
                     /gene="smpB"
                     /locus_tag="PLE88_00650"
     CDS             67905..68375
                     /gene="smpB"
                     /locus_tag="PLE88_00650"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOM75008.1"
                     /translation="MGKQSKQNPAPSVQNKKAWHNFELVEKVEAGIVLLGTEVKSLRE
                     RQCDLDGSYARLADGEVWLLGCKIAPYERGGYTNHEPLRKRKLLLHKNQIRKIRTKLE
                     QRGFTLVPTRIYFNDRGKAKVELALATGKKLHDKRQALREKQARKDIGRDMKYK"
     gene            68400..68774
                     /locus_tag="PLE88_00655"
     CDS             68400..68774
                     /locus_tag="PLE88_00655"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOM75009.1"
                     /translation="MADNGKKIIIDEDWKAQAQREKEILKEQEKREKEQASYPGLPSA
                     DFAGLVSMLATQAFYALGVIGPQDGREEDRQPDWAMAKFNIDMLGVLEEKCKNNLSDE
                     EDKLLKGTLGQLRMLFVQLSEK"
     gene            68778..69593
                     /gene="trpC"
                     /locus_tag="PLE88_00660"
     CDS             68778..69593
                     /gene="trpC"
                     /locus_tag="PLE88_00660"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOM75010.1"
                     /translation="MASILDKILSDKRAEVQLRRQQQPIELLMEKAMGLKKCRNFYQA
                     VTKPNPRGLNVIAEVKKASPSAGLIRADFDPVQIARIYQRCGADAISVLTDEPYFQGK
                     LEYLSQIKEAVCLPVLRKDFIIDPYQVYESRAAGADAILLIAEALTPSMLMDLLILAN
                     ELTLTVLIEVHNADTLLQMRSIIGFPQKNYSVLGINNRDLATMKVDLNTTGRLAELVD
                     NRRELIAESGIQTRADVEKLIRIGIGGVLIGQTLCQSPDIPSKFNELFGSCEG"
     gene            69596..70405
                     /locus_tag="PLE88_00665"
     CDS             69596..70405
                     /locus_tag="PLE88_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOM75011.1"
                     /translation="MSILIFFAIGLAAGVLGGLLGIGGSIVIIPALTILYGENQHLYQ
                     ASAMICNFFVSAAAIIAHWKAESFDKTVLKWMVPLAVLGIWGGVALSNCTIFAAENSY
                     LLARVFGAFLIYVAVYNIRHLYLHWRGKNITLHGPPVASMAHLVLSGFCGAATGLAAG
                     LLGIGAGTIATPLQQLLLRMPLCKAMSNSAGIIVAIAWLGAIYKNLTLAQHGLHWSSS
                     LHIAIFVIPGSIAGGYIGGHLMHLLPRNLVRFVFILVCALAAAKLLTVCPA"
     gene            complement(70436..>70617)
                     /locus_tag="PLE88_00670"
     CDS             complement(70436..>70617)
                     /locus_tag="PLE88_00670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOM75012.1"
                     /translation="HFLDCQGAEPDKIGYAVFGRVVEGLDVVDKIAAVKTTRKGFYND
                     VPVEPVEILSVRVIE"
BASE COUNT        18574 a        16687 c        17546 g        17810 t
ORIGIN      
        1 agcgggctgc ttggggctat ggctccgatt tgacgcggga atcgaaaaaa ctttcccaca
       61 aaaacaagtt gtctttgaat tgtataaatc atttcaggac aaaattatac ccaaaataaa
      121 acatttattt taaacgccta ttatattgaa ttccagtcca aaattaacaa agttcttgca
      181 ttttgttaaa ctcttttata ggattctccg tatagttagc aagtgtcagg ggagcaatgg
      241 gcttaaaagt cccagagctg agagtacgcg attgtaaagc agacccttag aacctgatcc
      301 ggttaatgcc ggcgaaggga gccacacgaa agaaagcatg tgcgcccctg ctttggatcc
      361 ggcaggggct tttttttggt acaaaaaagg tctgccctta agacatagtg ctgtgtataa
      421 ttaaaaaaga ggccaagaat ggcattgcct gtatcattcg agttaaaaaa tgctgttgta
      481 ggtatcgcag gtctgggcgg gcttgggtct aatgtcgctg cggcattggt tcggctgggg
      541 gtcggcaaac ttattctggc agattttgat gttgtagaag aaagcaattt aaaccggcaa
      601 aactattttg cagaccagat tggaatgcat aaagtagatg ctacgttaac aaatcttcgc
      661 agaattaatt cccagattca attgcagggg cacaagattc gcttaacagc tgagacgatt
      721 ccagaagtat ttaaagactg tcaggttatt gcagaatgtt tcgatcgtgc agatcaaaaa
      781 caaatgattg ttgaaactgt tttgtctaaa atagccggtg ttgttatcgt aactgtcagc
      841 ggtctggctg gttatggtcg gagcaatgac attataacac agcgcatttc agaacgcctg
      901 atactagtag gagatactgt gtctggaacg ggccccggag ttcctcttac cgcaccgcgg
      961 gtgggaattg cggcttgcca tcaggccaat gctattgcag aaattcttct caatgaaaag
     1021 tgccaaagaa agaaaaccgg atgagtgcga aattaaaaat taacggccaa aacaagatat
     1081 ttgaaggcgg tctgcccgaa accttaggga aactgcttga cgatttagca atcaatcaag
     1141 ccaccgttgt tgcggaaatt gacggttgca ttattgaacg caaagatttt gctcgtacgc
     1201 cgcttaagga aggccagacg attgaattga ttcgttttgt cgggggcgga tagatcgaaa
     1261 cgaacagagc ggatgcagac cattatgaag ccattgataa ttgcagatag acaattcagc
     1321 tcccgcctgt ttataggcac gggcaaatac gcctccagcc gaattatggc tcaggcgatt
     1381 gaagccagcg gtacagaaat ggtgacggtg gcgcttcgcc gggtgaatat cgatgatgaa
     1441 aacgacaata tgctggccgc aatagaccga aatcgctgcc agttgcttcc caacacatca
     1501 ggcgcacgcg atgcagcaga agcagtgcgg ctgtcttgcc tggcccgggc tgcaagcgga
     1561 atctgctgga tcaaactgga agtgaccccc gatccctatt atctcctgcc ggatggcgaa
     1621 gagacactga aagcggcaga ggttctggta aaggatggct ttgtggtgct gccctatatc
     1681 aatgcagatc ctgttctggc taaaaaactt caggatgtcg gttgtgctgc ggtaatgccc
     1741 ttggcaagcc ctatcggctc taatcagggt ttgaaaacaa aggcatccat tgaaattatt
     1801 atacgtcagg catctgttcc cgtaattgtg gatgctggtt taggtgcccc atcccatgcc
     1861 gccgaatcaa tggaaatggg agcggatgcg gttttggtaa atacagccat cgcaacatcc
     1921 gctgatcctg tacaaatggc agcggcattt aagatggctg tcgaagcagg gcgcattgca
     1981 tttgaaacag gccttccccc tcaatatgcc gccgccaatg cgtccagtcc cttgacaggt
     2041 ttcttgagag atgagctgtg agccgtcaga cgacaatcca aacgattctt gctgaaaagc
     2101 atctggatca aaatccagaa tgctggctgg ggacgcttgg aaatatcaaa accgatcagg
     2161 tcaggcagga attgtctaaa cctgcaggtt ttttttcttt cgaccgtctg ctggttttgc
     2221 tttctcctgc cgctgaagag tatcttgaac agatggccca gcagtcccag cgtttaaccg
     2281 tccaaagatt tggcaaaacc attcagctgt atgccccgct gtatctatcg aacgtctgcg
     2341 tcaacagctg cagatactgc ggctacaata gaaccaacgc aattaaacgg acacgactga
     2401 ccatcgagca ggcgcttgca gatgccgagg taatagcggg cgagggattt cgccatttac
     2461 tgttggtcag cggagaggat agagcattta ttacaacaga ttatcttatc aagttggctg
     2521 aacatctcaa acgctggttt tcttccttgt cgattgagat ttaccctatg gctcagaaag
     2581 actatgaatc tttatttctt gccggcattg atggggtaac attatatcaa gaaacctatg
     2641 atcgtcgtgt ttatgcccaa tatcatctgg caggtcccaa agccgattac gattatcgtc
     2701 ttcagacacc agaccggttc gcatctgctc agatgcgccg tatcggtctg ggggtgttgt
     2761 tgggattagc cgattggcgg ctggaaacac tggcgctggc agaacatgcg gcttatttga
     2821 tgaacaaata ctggcggtcg caggtctctt tttcgtttcc tcgtattcgt cctgcccgct
     2881 gtgtgcatgc tcaatggccc catttggtct cggataaaaa tcttgttcag atgatgctgg
     2941 ccttgcggct gtgttttgcg gatgcgggaa tggttttatc tacccgtgaa agtgcaactt
     3001 tccgcgatca tgtgattggt ttgtgtatta cccaaatcag tgcaggttca aaaacaaatc
     3061 ccggcggcta cagcagcgcc aaacaagcag tcgaacagtt tgaaatcgcc gatacgcgaa
     3121 cccccgctca agtggcccag atgatttgcg cacatgggta tgaacccgtt tggaaagatt
     3181 gggatgtgtc tttcagcaaa tcctgttgac gcagcgaagg gcagttcgta tgatagtgct
     3241 catccggttg tagtttcata aggtagaaaa tggctaaaat tcattttgat acacatcggt
     3301 gcaaaggatg cggactttgt gttttattct gcccacagaa aaatctgcga atgtctgaag
     3361 acctcaatga tcagggactg tcttatccgg aagagattaa tccagactgc tgcacaggat
     3421 gcggtttgtg cttccggatg tgccctgata cggccattga ggtcgtcaat gacaaaacgg
     3481 acaaacaaga tgtataatga atacgtcctt cgctagacaa agatatgttt gcggcttata
     3541 aatgtcggag ctgctgattc aaggaagtat aaataagcat gggccaaaag atattgctaa
     3601 aaggcaatga ggtaatggca gaagcggcta ttcaggcggg atgccgtttt tttgccgggt
     3661 atcctattac ccctcaaaat gaagttccgg aatatatggc atcacgcatg cctgaggttg
     3721 gaggtgtctt tattcaggcc gaaagcgaag ttgctgcaat taatatgatt tttggcgcct
     3781 ctgccgcagg ggcacgctgt atgacaagtt catcctcgcc gggcatcagt ttaaagcagg
     3841 aaggcatcag ttatatcgcg gccgctgaat tgccgtgcgt gattgctaat atgcagcggg
     3901 gcgggccagg gctgggaaat attcgagcct cacagggcga ctattttcag gctgtaaaag
     3961 gcggcgggca tggcgattat cggcttattg ttgtatctgc cgcaaccctc caggaacttt
     4021 acacactgac gatgaatgcc ttcgactggg cagattacta tcgcaatcca gttttgattc
     4081 tgggcgatgg cattctgggg caaatggctg aaccggttga gattctgccc tatcgtccgg
     4141 tactggagat tcctccaaag gattacattt taaccggctg caaaggacgc aatcctcgtg
     4201 ttattaaaac tctcatctta gatcctgctg aaggattggt acagcacaat gaacatctgc
     4261 aggagaaata cagccgaatc gagcgggaat tgtgcctgta tgaaacctat caaattgaag
     4321 atgcagaact gattgtaacc ggatttggca taaccggacg cattgccaaa gcggctgtcc
     4381 gcaaagcccg ccagaaagga ctggcagcgg ggctgattcg gccaattact ctatggcctt
     4441 ttccaaagaa gatttatcaa caggctgcaa tgacggccca gaaggtactg gttgtggaaa
     4501 tgaaccatgg ccagatgatt gaggatgtca aacttgcaat ggactgtgct attccgacag
     4561 aatttctcgg caaagggggc ggttggtatc cagacgaaaa agccgtgctg gaggcaatcg
     4621 agtcaatgct ggagtcgcct gcggcgtcgg tgaagggagg ggcatagctg tggcagtttt
     4681 caagcgaacg acggtcttga aagaacagct gactcactat tgccccggct gcggacatgg
     4741 tattgtgcat cgccttgttg ctgaagtcat cgacgaattg gggattcggg agcaaaccat
     4801 aggcaccgcg cctgtcggct gtgctgttct tttatacaat tatttcaact gtgatatcat
     4861 agaatgtgcc cacggccgtt ctccggcggt agcgaccggc attaagcggg ttcatccgga
     4921 tagaattgtt tttgcctacc aaggcgatgg cgatttggca gccatcggta ccgccgaaac
     4981 gattcatgcc gccaatcgcg gtgaaaatat taccgtcatc tttgtcaaca acgccattta
     5041 tggtatgact ggcgggcaga tggcccccac aacaatgccc gggcaaatta cgacgactac
     5101 tcctcaggga cggggctgcc ataaagaagg ccccccgata aaagtatgtg aattgctggc
     5161 agcactggat ggagtatctt atttggagcg gacagcagta agcagcccga gggatgtcat
     5221 gaatgccaag aaggcaatta aaaaggcatt ccaaaaccag atagagggca aaggattctc
     5281 gctggtggag gtgctctcaa tgtgtccaac ggactggaag ctgtcgcctg ctcaatccgt
     5341 agagtttgtc aatgagaaaa tgaagaagat tttcccgctg ggcgtattca aagaccaaca
     5401 gatatgaatg aaaccaaagt tttagtagcc ggcaccggcg gacaaggtgt tgttgtcatc
     5461 ggaaacatcc tggcacgagc cgccgtaatt gaaaacaaga atgtggtcgg aatggttgcc
     5521 tatggggctg aaatgcgggg cggcacggcc tatgctgcca tcattataag caatgaggaa
     5581 atcggcagtc ccattgttga ccgtcctcat attgcgatta tcctcaacca gccttctctt
     5641 gacaaattcg aagaggatat tcttcccggc ggattggccc tgataaatac ggcaatggta
     5701 gcaaagccgg tcaaacgcct cggtctgcag cgaatcgaag tggctgctac ccaaattgct
     5761 catgatttgg gaaatgtcaa ggtcgctaat attgtagcta taggagcatt gataaagcat
     5821 acaaacctgc tttcaatgag cagtgttgag cagggtctta ccgatttatt tacagccaaa
     5881 aatcccaaat tggtcccgct gaacctgaaa gccctgcacg ccggagcaga acacagtgtc
     5941 tatgtaccca gttctgtacc ggtggatatt tgagatggac agctgtcaca aatgaatgag
     6001 tactaaacag aacatatctg ctttttggca gactgctata ggtgcagtaa actcccgtcg
     6061 gcggcttccc atcggaattc gaatgacccc gatgatagat gtgatttttt tattgttgac
     6121 cttttttgtc ctgactgccg agtttcaaaa acccgaacat acgctccctt tttctctgcg
     6181 aaagacaggt acccatgctg cagcagtccc gaatgaaaca gtaaaaatat acattaaatc
     6241 ccagcagaat acctgtctga ttgacattga caataggctt gagattaccc ttgatgccca
     6301 aaatccgcaa caggggcttc tgatgctggc acaaaacatt cagcaaataa cggaaaactt
     6361 tggtgcagta ccgatagaac tatattgtca ggattcagtc ctatgggatt atgttgtcaa
     6421 aatttatgac gtattatatg ctttgggggc ttattctatt acgttccgca tcgaagaatg
     6481 atgctggaag ttaattttaa aagcgtttgt tgcaggcaaa atccctgtct gctataatat
     6541 ctgcagattt ttttctttga gaagcaatcc tgacaagaga taaatcataa aacaggagtt
     6601 tttatatggc aaaatcatcc gggcagggat cccggcggga gatgtgcagt ttttgcggcc
     6661 ggaccaaacg gcaaatggac gcttttgtag agggtccggg cgggatcttt atctgtccag
     6721 actgtataga cctttgttat aacattgttc gtcaggaacg gcgaaggtcg cacggtgcag
     6781 gcacattgtt tgaagaggtc cccaagcccc gtcaaatcaa agaatttctc gaccagtacg
     6841 tcataggtca ggagcatgcc aaacgcagcc tatccgtagc ggttcacaat cattacaaac
     6901 gtctgctgca tgccgacagc gataatgacg atgtcgaaat tgaaaaatcc aacatcttac
     6961 tgattggtcc aaccggatca ggcaagacct tgctggcccg tacgctggca agaatgcttc
     7021 atgtgccgtt tgctatttgc gatgcaacca ctgtaactga ggccggctac gtcggcgagg
     7081 atgtcgaaaa tttcctgctt cgtcttttgc aggcggccga ttatgatatt gaggctgctc
     7141 agcggggtat tgtctatatc gatgagatcg ataaaatagg aaagaccaca cagaatgttt
     7201 caattacccg cgatgtatcc ggcgaaggtg tccagcaagc cctcttgaaa atgcttgaag
     7261 ggacgattgc caatatcccg ccgcagggcg ggcgcaagca tccggagcag tcctacatcc
     7321 agatggacac cacccagatt atgtttatct gcggcggaac atttgtcggt ctggacaata
     7381 taataaaaaa acggctgggc aagcggatga ttggttttga cagtgagcat caggcacacc
     7441 acgataaaaa agccgactac agcgaaatta tctcacaggt tacgccggaa gatattatct
     7501 cctttggaat gatcccagaa atggtgggac gactgcctat cattacaacc ctcaccgccc
     7561 tagatgaagc ggctttggta gacattctga ccaaaccgaa gaatgccttg gtccgccaat
     7621 accagcggct tttcgaaatg gaagacgccg aattaaaatt caccgatgag gcgctggatt
     7681 tgattgccaa aaaggccatc aaacgggata ccggtgcgcg ggctttgcgt gcaattatgg
     7741 aagaattgat ggtggatctg atgtaccgcc tgccggaaga gcctaagccg ggcaaatata
     7801 tcatcacgcc tgaggttgtc gaaggcaagg tcaatctctt cgaacaagcc cgcaaggtca
     7861 aaaaagaatc agcctaacgt caataatctg ccatctccgt tcttatgcaa gacgaatgtc
     7921 agacattttg tattaggaat taacggccgg aggcggtcgt tttttcagtc aatcagataa
     7981 agccccgcag gacattacac cctgcagcag gagaaagatt ttcgtctctt atgtattgag
     8041 aagcaatccg gttttgtgta atatatcccc catatttaaa tctgtcggac ttgatccagc
     8101 agtcagccag ttaaagacct gaaatctggt ggccaagttt tttttgagcg gttgtaagga
     8161 aattgcggat ttataaatgc tttttttcct aaaaaggata cgatgtgaag cagtggcgat
     8221 ttgaaatttc atcaaaacag ggatttttag atgtccacgg cagggatatt ctggatgaca
     8281 ttcggcagtt taatatcctt tccgttgaag acgttatatc tgaaaaggtc tatttgatag
     8341 aagcagattt tgaccagccg ttcgccgagc gggtggcccg cgaattgctg accgatccag
     8401 tctgtcaaga gtggcacatc ggcaaaagcg gcattccggc cggccccatg cctgcaaccc
     8461 ttatagaagt acaccttaaa agcggtgtaa ctgacccagt cgctgaatcg gtaatggccg
     8521 ccttgaaaga catgggagca ggggcagtcg aatcagttcg tacggcccgc aagtatgttc
     8581 tgctgggaaa tgtaacagaa aaagaccggg aaacaattgc acgtcggata ttagccaacg
     8641 actgtatcga agaggtcatt tatgggatgg atgccgaacc tccaagcccg cataccaagt
     8701 cctatgaact caaccttata cagattccga tttgcggact cgacaatgaa cagttagttc
     8761 aattaagcaa aaagatggat ttgttcctga atcttaaaga aatgcagacg attcaggcct
     8821 attataggga aattggccgt gagccgacgg atatagagct tgaaacttta gcccagacgt
     8881 ggagcgaaca ctgcgtccat aaaacactta aaagcagtgt tgatatggac ttggacggcc
     8941 ggaaactgca ttttgacaat cttctgaaag atactgtttt taaggcgaca aaagagctcg
     9001 ataaaaagtg gtgcatatcg gtcttttctg acaacgccgg agttgtcgaa tttgatgatg
     9061 aatacgctgt ctgtttcaaa gttgaaacac acaatcatcc atctgcactt gatccctatg
     9121 gcggggcggc tacgggcatc ggcggtgtta tccgagaccc gatgggcact ggtctggggg
     9181 ctaaacccat tgccaataca gatatttttt gcttcgccta ccccgatatg aaacttgaaa
     9241 atgttcccaa aggcgttctg catccccggc ggattatgaa aggcgtggtg gcaggggttc
     9301 gggattacgg caaccgcatg ggtatcccca cggtcaatgg cgccatttat tttgatgatc
     9361 gctacattgc caatccgctg gttttctgcg gcaatgtcgg cctgataccg cgcgataaat
     9421 gctttaagca tccgcaggca ggaaatctga ttgtagtcgt gggcggcagg accggccgtg
     9481 acggcattca cggagccact ttctccagcg gcgagatgac ccacgaacat gaaacaattt
     9541 tttcgcatgc cgttcaaatc ggaaatgcca ttaccgaaaa aaagatgctg gatactcttc
     9601 tgcaggcacg cgatgcaggt ctgtatgagg cgatcacaga ctgcggtgct gggggactca
     9661 gcagtgccgt cggagaaatg ggcgagaaac tcggagccga agtagattta gaaaaagtac
     9721 cgctcaaata tgcgggactt tcctatactg aaatatggat cagtgaagca caggaacgaa
     9781 tggttatcgc cgtcaaaccc gaaaacatcg agtccattat gaagattttt gcggctgaag
     9841 atgttgaggc aacggtcatc ggccgtttca ccggcgataa gaagctgacg cttcgttaca
     9901 atggtgttca ggttggccag ctggatatgc agtttctcca tgaaggcatt tgcaaataca
     9961 gccgaaatgc cgtatggaag acaaaatcat tgagcgaacc ggttcttcct tcacagaaaa
    10021 actttaccga ggaccttctg aagattcttg gctcctacaa tgttgcatcg aaagaatggg
    10081 tgattcgaca atatgaccat gaggtgcagg gcggcagtgt tattaagccg ttgatggggg
    10141 ctcaaaatga cgggccaatg gatgcagctg ttattcgtcc aagatttcat cacaacaagg
    10201 gaattgtaat cagctgcggc atgaatccgt gttatggaga tatcgatccc tattggatgg
    10261 ccttggcggg catcgacgaa gccgttcgaa atctgatttg cgtgggggcc aatcccgacc
    10321 ggattgctct gctggataac tattgctggg gcgattgttc ccgaccggaa atgctcgggg
    10381 ctctggtaag ggcttcacag gcctgctatg atgcagctat tgcctatgga gcaccgttta
    10441 tctcaggcaa agattctttg aacaatgaat ttgtctgcga tgacggcagc cgcattgtga
    10501 ttccctatac cctgctgata agcggcatcg gcattgtaga gaatattcaa caatgcatca
    10561 caatggatgt gaaaaagccc gggaatctga tttttattat cgggctgaca aaaaatgaat
    10621 tgggcgggtc acactactac aaaatacatg ggcatctggg agccaatgtc ccgcaaacca
    10681 acctgtcgca ggcacctgtt attgccagcc ggattgccga ggcaattcgg cgaggattgg
    10741 tacgttcctg ccatgactgc tcagagggcg gactggctgc tgcactggca gaaatggctt
    10801 ttgccggcgc cttcggcatt gaggccgatc tgcggggtct tccccttagt ccagattgtg
    10861 tcacgccggc agcggcactg ttcagtgaat cgactgcccg gtatcttgtc gaggtagagc
    10921 cgcagcatta tgatgcattt gcaaagctta tgctgaatgt gccgttcgga caattgggcg
    10981 aagtgatcga agacaaaaaa ctgctgattc gcgatacaca gcagaagaca gttattgaag
    11041 cagaattgga tatcctaaaa gaggcttgga agaagccttt ggcatggtaa gagaaaataa
    11101 gaaattcagg catgttgcga gccgggtgtt tggtctcttt tatatgttct tggcttgatc
    11161 aatgccgttt gcagaatagg gattttcaat gacaacagtg agggcaatcg tattaagagc
    11221 cgccggcatc aattgtgatg tagaaaccga gcatgccctg cagcttgcag gagcacagac
    11281 agaacgggtg cacatcaatc ggctgattgc tgcgcctgca atgctggacg actttcagat
    11341 tctggttgtg cccggcggtt tcagttacgg cgacgatgta gccgctggaa agattctggc
    11401 taatcagatc gtccatcacc tcagcgacat cctgcagcgt tttattgaaa acggcaagct
    11461 ggtactgggt atctgcaacg gattccaggt gcttataaaa acaggcattg tgccggggct
    11521 cagcggagca ggccggctga ctctggaggg ggaaggcgtc acgctgacca acaacgattg
    11581 cggtcattat ctggattgct gggtgcacct tgcacctgct acaactcggt gtgtgtttat
    11641 tgaccctaac cgccggctgt atctgccgat tgctcacggc gaaggaaaaa ttgtcgccga
    11701 cagcattgag cggctgcagc aaatccgcac cggcgggtat gtggcttttc ggtatgtcga
    11761 tgcggatggc caaacaggat tgtacccaat caatcccaat ggttctcagg attctattgc
    11821 ggctctgacc gattcgaccg gccgcgtact gggaatgatg ccgcatccgg agcgttttgt
    11881 ccgctggaca cagcatcccc actggacgcg attgaagcag caagatgagc tgactgacgg
    11941 catgacaatc tttaccaatg ccgtaaatta cataaaacaa actttttaag cagacgagga
    12001 attttcagct gatgattcaa atcaatgaaa attttttgaa attgaaggcc ggctatttat
    12061 tcccggaaat tgcaaggcgg gtatcagctt tcgctaaagc caatccgaaa gctgatatca
    12121 tcaaactggg cataggcgat gtcaccgagc cgctgccgca ggccgttata aaagcaatgc
    12181 ataaggctgt tgatgaaatg gccacccgag agggttttca tggatatggc cctgagcagg
    12241 gatatgaatt cctgcgcagc actattgcgg aaaacgattt tctgccccgc ggcgccaatg
    12301 tttctcctga tgaaattttt atcagcgacg gagccaagtg cgataccggc aatattcagg
    12361 aaatcttcgg acttgacaat gttgtagcgg taggcgaccc tgtatatccc gactatgtgg
    12421 atacaaatgt tatggccggg cgcacaggcg aaaatgacgg cacaggacgg tatgcaaaga
    12481 ttgtttatat gccctgcacg ccggaaaaca attttgttcc ccggctgcct gaagaacctg
    12541 tcgatatgat ttatctgtgc tatccgaaca atcctacagg tgctgtcgca accaaagaac
    12601 agctcaagca atgggtggac tatgctgttg cgcacaaggc cgttatcctt ttcgacgctg
    12661 cttatgaggc ctatatccgg gacgacagca taccgcattc catttatgaa atagaaggcg
    12721 ccagacaggt tgccgtcgaa ttccgcagct tttccaaaac agccggcttt acaggccttc
    12781 gctgtgcctt cacggttgtc cccaaagaac tggtagctta tacaaaatca ggccggccgc
    12841 agcccctgca tcctgtctgg aaccgacggc actgcacaaa attcaacggg gtcagctatg
    12901 tcacacagcg ggctgctgag gccgtttaca gcccggaagg caaaaatcag gtcaaagcca
    12961 tcatcaattt gtatatgtcc aatgtcgaca caatgtgcca gtctctgcag aaattcggct
    13021 attgtgtata tggcggcgta cacgcaccat atatctggct gcagaccccc cgaggctttg
    13081 attcatggag tttctttgat acgctgctgc atcaggccca tgttgtttgt accccggggg
    13141 cgggcttcgg cgctgccggt gaaggattcg tgcggctgag tgcatttaat catccggatc
    13201 ggattaaaga agcaatggaa cgtataggca agctgtaaac tgcaacgatg acagaacaaa
    13261 ccgcatatat cggactgggc agcaatcttc cggattgccg ggtcaatctc tgcaaagcaa
    13321 ttggtttgct ggggcgccac ggaaaggttt cggctgttaa ggccagttct ttttatcaaa
    13381 ccaagcctct cggtcctgtt aaccagcagg attttctgaa tgctgtcgtg caggtcaaga
    13441 cctctatgga tgcaaaagag ctgtttgact atctgcggga cattgagcag caaatggggc
    13501 gtcagaggca gcagccttgg ggacctcgaa cgattgatct ggatttgctt ttattcgaaa
    13561 accaaaccat aaatgagccg gatttgaaag ttccgcatcc gcagatgcat ctacgcagtt
    13621 ttgtgctgga tggcttatgc caaatagctc cggcagtgga acatcctgtc ctaaagcggt
    13681 ctatgtccga attggctaat cgtcttaacg gacgcagttt ccggctggat tcccaaaaag
    13741 cccagttagt ctcggtggca ggaactatcg gcgtcggcaa aacaaccttg gctgccggat
    13801 tagccaagcg gcttggggct gagtttatag ccgaacaata cgatgacaat ccctatttgg
    13861 ctgatgtcta tgcggggcat caggagctgg ctttggattc cgagctgttt ttcctttcca
    13921 gcagtgtgtc ccagttatcc aaatacaaac tgacagcggg aaattggtat gtcagcgatt
    13981 acgtgttcga aaaaggcata ctttatgcat ccagctggct gaaatccaaa gatctggctg
    14041 tgtacaaaga gcattattgt gcagtcagca gcactgtggc taatccggtc ctcgttatcg
    14101 ttatgcagga taccgcccaa aactgtcttg aacgcatccg ccgtcggaac cggccctatg
    14161 aacagcagat tgaaccggtg tttttagagc atctttcccg cggatttgtg cggttttaca
    14221 atcaatattc cggatgtccg gtaattcgaa tctcgccgga tgcctgctgg acagaccagc
    14281 agatagactg ccttgctgaa gaggttcgct ggtatcttgc aaagatggaa aattgaatgg
    14341 aaataatcca tacaatagag caaatccgcc aatgcgtaaa agcagcgcga aacagaaatc
    14401 tcacagtagg gcttgttccc acgatggggg cgctgcacga agggcacggc tcgcttataa
    14461 tagctgccaa gaagcaatgc ggctatgttg taatcagtat ttttgtcaat ccgacacaat
    14521 tcggccccag cgaggattat aaccgttatc ctcgtacttt tgatgccgat gcagcgtatt
    14581 gccgaaatct gggtgtggat gcggttttcg ccccttccgt tgagcaaatg tatcctgccg
    14641 aacagttggt ttgggtacat ccagagaaat taacagacca cttatgcggg gcttcgcggc
    14701 ccggacattt tcgcggtgta gcaaccgtct gcaccaagct gtttaatatt gtacagccgg
    14761 atatcgctta tttcggacag aaagatgcac agcaggtggt tgtgatacgc cgaatggttc
    14821 aggatttgaa tattccgatt caaatctgcg tctgtccgat tatccgcgaa ccggatggtt
    14881 tggctatgag cagccgaaac cggtatttat ccccagccga acgccgtcag gccttgtgcc
    14941 tttacaatgc ccttacagca tgtcgcagcc gaatagccgc cggccagaga aatgctaagc
    15001 tgctcattga tttgatggct ggcatcatag cagacagcgg cggacgaatt gattacatca
    15061 gtattgtcga tccgcacaca cttgaacctc tcgaatatat tgagcaaaca gctctggtcg
    15121 cattggccgt ccatattgga aatacccgtc tgatcgataa tcttctaatc gacttgaaca
    15181 atttgtctgg gcctgtataa taatgcctaa ctgcttttca ggaataatgt tatgttaatc
    15241 aaagcgttaa aagggaaaat tcaccgctgt cgggtcacgc agaccaaaat tgattacccc
    15301 ggcagtattg ggatagatgc acaaatgctc gctgcggccg gcatttcccc gtacgaagaa
    15361 gtgctcttgg ccaacgtaac caacggcgaa cgcgtggaaa cctatgtcgt tccggcccca
    15421 gccggcagcc gagatatcat cattcttggc gcggcggccc gattattcag ccccggtgat
    15481 attgtgatta ttatgaactt tgcttattac tctccagagg aattacaaaa tctccagccc
    15541 aaagtgcttg tctgtgacga acacaataat tattctgtcc tgcagaaatc gtaaatgcat
    15601 ccggaacttt ttactatacc gtttctgaat atctccgtca aaagctacgg aacaatgatg
    15661 gtcattggtt ttctggcttc tgtatggctg atgcggcgga tgatgaaccg tatgggtcaa
    15721 aatcctgaat ggataaccaa tatcgctatg tatgcacttc tctggggcat tgcgggggca
    15781 agagtttttt atgtcgttca tcataatgat gtatttcgca gccggccgct tgagatattt
    15841 gctgtctggc agggggggct ggaacttctg gggggagtgc tgacagcgat tgcttttttg
    15901 ggattctatc ttcgccggag aaaacttcca atagtgctgt atctggatgt gctggccgtc
    15961 gggcttatgg tgggtttggg attcggacga atcggctgct ttctgaacgg ctgctgctac
    16021 ggcaaagtaa ccgatgttcc ttgggcggtt cgttttccct acggcagccc ggcattctat
    16081 ggtcagatac agcccgaccc ctcacgcgga cgcagccaac cgcatatgga gctgcccgat
    16141 tcgtattttc aggacgggta tctcaagccg ttggcgttgt tgacgcccga acagaaacag
    16201 gccatttctg atggtgctgt ctgctctttg ccggttcatc ccacacagat ttattcgagc
    16261 atcaacgctt ttctgattgc aggcatcctg tatgctctct ggatgaaatt cgccgagtct
    16321 cgacccggca tagtgatgag tttaatgctt gttttttatg gaattacgcg catcttcctt
    16381 gaaagtctca gagacgataa tccgtttgaa tataattggt gggcagtcta taaaggcgga
    16441 acagtctcgc agaatatctg tatctatctg attttaaccg gtattatcct gctgatttac
    16501 tttatcacac gaacaccgac acgactaaaa actcatcgct aacggccatc cgtcttcttt
    16561 ttgcgagtca tttttcctga ctcttccata tagcttccgt caggcataag aatgcgttcc
    16621 gttgcaatcg cgatgtcgtt attgattttc tctatgatcc gtgtgctttt gtgacctgtc
    16681 gcccatttcc aatccataat ctcccgcttt ccgtcgcaag ttcctctgcc ttcggcgatg
    16741 caccgcaagc tgtcaaaaag atatccgatc gtctctcctg tgcgctggtc aattgtataa
    16801 acctccagcg agcaaaaggg ctgattctta aatcgctcta tttcttcgtc cgaagcatga
    16861 agctgctctt tgaggtactg cagttgttcc ggcgttatct cggtgaccac ggcagtacct
    16921 ttgtgaaagc gaaactgccc gttaagtgcc agctcggtcc gcccctcgcc ggtgccatac
    16981 cccgcatcgg attagcccgt cagttcccaa tcgcccacaa gccaagtgct ccacgcatcc
    17041 tgacggggca gaggattaaa cctataattg gcggattcaa cgatagtggg agtatctttt
    17101 gtaacccgat ggcatgatat gctaaagaat gcaatcacaa tggccgacat tgtaaggatt
    17161 gaacgtatca taataaatct ccttaatttg gacggcctgt attataccct tccaaaaata
    17221 aatgcggcca gtaaaaaatc tccaataaat cgattagggc cgcatataaa aacacggatt
    17281 agataatccc ttccagtgcc tgtattaatt ctgtcaggtg tttttctgta tgggcggact
    17341 ggacggagac ccggaggcgg gcggtatttt gtggaacggt aggggggcgg attgcactaa
    17401 caaaaagacc ctgctcataa aggcggcggg ataccgctgc tgtttctcgg gaatccccta
    17461 ttataacggg gataatgtgg gttgtgcttt ggccaatatt caatcctaat tgctggaatt
    17521 gatttcttaa ataagctgca ttgttgtgca gacgcttgcg ccgttccggc tcggcttgaa
    17581 taatgtctaa tgccttcaat gcggcagcgg ctatggcaca agatggggcg gtcgtataaa
    17641 taaaagaacg agctcggtta atcagatagt ctattaccgt ttcaggcccg gccgcaatgg
    17701 ccccgctgac acctgccgct ttacccaacg gcacgatgag gatgtcaata tccgacaaga
    17761 ggccttcttg ctggcataaa cctgcacccg ccgaaccaaa gcagccgaaa ctatgggcct
    17821 cgtcgacaat caagatagca ttatacttct tcttcagccg tactaattcg gccagattgg
    17881 cagtatcccc atccattgaa aagacgcttt cagttacaat gaagcggttt ttatagtttt
    17941 tttcgctcaa atacttttcc agacgctcca gctgattgcg tcgataggta tgaaaaatcg
    18001 cctcgcatgc tctggcagca tcgataatag aagcgtggtc aaatttatcc agcaaaacaa
    18061 tgtcgccttt ctgcggcagg gctgtcaaaa ccccctgatt tgccatccag ccggaaggga
    18121 aataaagagc agcttctttg tctaaaaatg cggcaaatgc ctgttccagt tctgtatgcg
    18181 gcagctgggt tccacaaatc agccgtgagg ctgacgctcc aagaccgtac cgctgaatag
    18241 cctctatggc tgcatgacaa atcctttgat cttctgccaa attaagatag ttattgctgc
    18301 agaaaagcgt ttttttgctt ccgtcggcca tttgtaccgt cggcccctgt gctgaatcta
    18361 tgcatacaca ccgccgcaac agaccgtcct gctctaagtg agccagctga tttcggtaaa
    18421 aatcaaaggt atccatcatt ttggcatttt actattgaac ccgtttcaat tttggtagat
    18481 attataatca ttgatttgcc taattatagc atattttagg aaaccaaatg ggtatttatg
    18541 acagagatta cagcaggcca gttcgccaag agctgtatag catggggatg ggctttcgga
    18601 cattgccgcc ggtggtcaaa tggctgctga taggcaattt tgcaattttt gttgccgcta
    18661 atctgcttcc caaagcgggt aagcagattt accttttcgg ggcagttctt cctgttaacg
    18721 ccgtttatat gattcagctt tggcgggtta ttacctatca atttctccac ggcggggtag
    18781 gccacctttt gtttaatctg atggtgctct actttatggg gccgttggta gaatgccagt
    18841 ggggttcaaa agcattttta aagttctatt tgatttgcgg agcagccgga ggaattgtat
    18901 acacgctctt ggtgctgctt catgtgcttc ctgccgctat gatggttgga gcatcaggag
    18961 caatatacgg cgtcatggcg gcgcttgcgg ttatgtttcc gcaaatgagg gtgctgctgt
    19021 ggggaattat acctatgaca atggtccgtc tgattatcct gctggttatt gtgagcatgc
    19081 tgatgattgc cttcggggaa aatgccggcg gcgaagctgc ccatctgtcc ggtttggcaa
    19141 tgggtttttt gtatatgaag gtcaaacctt gggcggtgcg gcttcgcatg gaacgcagca
    19201 agggggcttg ggcaagaaaa atagagcagg acagactctt tcaggcagaa gttgaccgta
    19261 tccttgagaa agtgcaccgg cagggaatcc attccctcac cgcacaggaa aaacagactc
    19321 tccaaggagc cacacgtcgg gaacaggaag cgtcggtaaa atcttctctg caatagaaag
    19381 agcggttcct gtttttgtga ggctgtttta gccggaagaa atgtttctta atccatatta
    19441 tccgctgctt tgtacttatt cctgtcagga tttttttcag tttatcttga aatcagtgtg
    19501 gtcttgtgtt aaaaatttcg gccatgcaga ctttaagaaa caaaatagta tgcctgagcg
    19561 gctttattgg cctggtcgtc ggctttgcgc agggcgatgt ggttcaggat cttattgtac
    19621 gtcataaaca gacgaaaacc gtattttcga ttatggctgt cgaagcgaaa acgggggctg
    19681 ttctttacca gctccatcct gacaagccca tgatacccgc atctaatatg aagctgatta
    19741 ccacttcggc ggcgctgcac tacttgggcg gggattatat attcaaaacc aaagttggaa
    19801 ttctcggcag cgatttagtg attgtagggg gcggggatcc tctgttggca gatcccaaga
    19861 atgattccgc acccggccaa gcagccacgc ttttgataag cagaattctc ttggcagtca
    19921 aagattccgg aatccgccag attgataata ttattgttga taccagcttc tttgacaaca
    19981 acagagtcca tccttcttgg ccgaaagagc agttgaatca gtggtacgcc tgcgaagtca
    20041 gcggactgaa tttccacaac aactgtatcc gattaacggc tgcccgaaat ggaaaagaag
    20101 ctgctctttc aatgatacct gaaaactcat atgttcatct gattaaccag atacaagtcg
    20161 tttcaagcgg gaccagtgcc gtaggagcct atcgcaattc cgtccccaac agactgctgg
    20221 tgaaaggcaa gcttaatcag gcagccggat ttgatgtggc cattgaaaat ccggctggac
    20281 ttctggcttc cattgtaaaa gatcggcttc agaaagaagg aattccggtt cggggacaga
    20341 ttctgcagca gtatattaag cagaatgagg cgatacgcta tctggttatc atagaaaccc
    20401 ctattgccga agtgctgaaa cgctgtaaca ccaacagttt gggtttagct gcagaatgtt
    20461 tggtcaaaac catttcggcc gaaaacacgc agggacacat caacggagaa tggccgcatg
    20521 gattaaatct gatttctcgg tatctcgact cactgggtat tccaaaagaa caatacgttc
    20581 tggatgacgg cagcggattg agccggaaca accgtttgtc cgcgtactgt ctcatcacgg
    20641 tcttgaaaga catgtatttt catccggatt catccatttt tctgtcatcg ctggctgtcg
    20701 gaggccttga gggaacagcc gaaaaatatt ttcaggaacc cccttacaag ggtacaattc
    20761 tcggaaaaac cggctatatt gccggcgtgc gctcgttttc gggcatctgc agaactcccg
    20821 ccggcgatat cctcttcagt attctgtcag aagggtcaag cagtgcagcg aggtcctgta
    20881 tcaatgacat tacaaaagcc atctacgaca gaaaattata gacgcctgtt tcagactgcc
    20941 agttcgcctg tcaattcctc aatcaaatcg gatattgtaa tcagcccgac ggcgcgtctg
    21001 gttcctttgc tgtaatctac aaccaaagcg atgcggtcgc ggcgattccg cagaatattt
    21061 atagcttcga tgatggaagc agtccggctg acttctgcaa gaggcatcac ataggggcgg
    21121 actgtctcaa agtcttcctt gctgctcagt acatcataaa tggaaagata gcctacaata
    21181 tcagatggat ttcggtcata gacaagctgc cgtgtaaacc ggctttgcct gaggtgccgg
    21241 agcaaatcct gacgtgtcat catcaagggg attttgtccg ctttttcgat aggaaccatc
    21301 acagaagcag cagacacgcc gggtatatca attaatcggt taatcatctc tcgctgcgtc
    21361 tgagaaagca atccttcttc ctgcgtttca tggataatct ggcgaacctg ataacgctgg
    21421 gtcttatcaa cggcttctgt tgtatttgta cttaggttta aaatcttcga aaaaaacagt
    21481 gaaagatgtt tgaaaaccca aagaagtccc gatcggcaaa agaggcgatg gatgtaccag
    21541 atgccccaag ataagcgggg caaaagcgtg tcggccttgt agtagaaaat atttttggga
    21601 atcatctcgc agaaaataaa aagcaggggg gccagcagaa tcgctgcata aatttcggct
    21661 ttgtgctgat tttcaagctt gcggtaaaga aggattgtga cgatacttgt aatcaaataa
    21721 tttacacaat tattgcccag cagcagggac aagataagca tcgggccatc attgaccgct
    21781 ttcgacagga tggtgtaggc cttgcgtttc tgctcaacgc ccagccgcag gcggaatcgg
    21841 cttaaacggt aaacaccgat ttctgcccct gaaaaaagac cgctgaaaat cattaaaaat
    21901 actgcaaatc caagtccaat ccaaaccatc agtgcgcttc cattgaatcg gaattcacaa
    21961 gcggttccag attcaatatg accgattgaa tccggttgtt tttcacttgc tctatagtaa
    22021 acgtcatatt cttaaaccgg attttatcgc cggttttagg aatcttgccc aaaagggctg
    22081 ttacaaatcc cccgatagtt gtcagccggc cctcatccag attaatcccg aaggcctccg
    22141 accaatcata aatggccaga tccgccagca gccggtattg cagaggcccg atggcttcta
    22201 ttggattctg ttcaggcagt tcttccatcg gaccaagaag atgttctata atatcttcca
    22261 gctcgacaaa tccggaaacg cctccgtatt catctacaac aactgcatga tcagtctggg
    22321 tgtttttaaa aaaaccgatc agcgattcaa ctgtcttctg ttccggaaca aagtgaacct
    22381 tccgcaccag acaagccaga ggctgctggg aatttaagag ggtatcacac agatgtacca
    22441 taccgacaat gtcatcgact cctgttgcat aaactgccat cttatcgatt cggcgggcaa
    22501 tcatttcatc tctgacagct gccgtttgcg aagcaatcga acaaatcggc atttccaccc
    22561 gcggcagcat aacatggcgg acctttaaaa aactaaactt taatatctcc gtcagcagca
    22621 ggttttcatc attgctgatt aagccctgtc gccgggacga ttcaaggagt attttcagct
    22681 gatttattga aatagaaggc gtttgctgat gacgcacaaa cagccgcaag ctgggctgca
    22741 ccacaagcca atccaaagct ctcaggatcg gtttcagcga agtcagcaca atataacaaa
    22801 ccggcgacgc ccacaggcaa aaccgaacgg tattggcata agccagcgtc ttgggcagta
    22861 tttctccaaa cagcacaagc atcataaagc agatgattcc tgagatggtt cctgcagccg
    22921 gaccataccg atgaccaatt ttaagaagca gtatgcttga accggcaaaa aaaagcacat
    22981 tcacaatcat attgccgaac agcagggccg ttaaaaagcg gttgggatct ttcagaattt
    23041 ccgcaatcag ccgttcaatc ggcaaggtcg aatgggcaaa ctgacgcact gttttgcgcg
    23101 gaagctggaa aaaagccgtt tcagaacctg aaaaaaatgc ggaacaggcc agcagtcccg
    23161 ccatccaaat catctgcagg ccgtaatcaa acacaaatga cgccaaaaca gcagtcgtgg
    23221 gcatgaattc aatccttttt gggaagctgc ttttaagatc tctgttttgg cagcagaatc
    23281 ctaaagcggc tgccttgtcc caacaccgaa tctgcagcaa tgcagccgcc atgcatttca
    23341 attatctttc ggcagaaagc caatcccagt ccattgccgt tttttttgcc ggatgtgtaa
    23401 aaaggagtaa aaagccgttg caggtcatcc ggtttgattc cgcagcccgt atccgttatt
    23461 tcgattcgga taaattcagc cccgtcatcg gcagcaattg tcagtacgcc tcctttttca
    23521 cgcatggcct gatgagcatt cagtatgagg ttcatcataa cctgcctgag agcatcaaca
    23581 tccccataaa tgaagagtgc ttcaggaacg tgtcgaacaa cagttatttt gtccttggaa
    23641 aaatcacggc ctaaacatcc aaacacatca tccagcaagg atgtgagaag gcatttttta
    23701 ttctcctgcc taccggcacc tgccaaaagc ataacttttt ccagtatttg cacagcttgc
    23761 ttggttaaat acagtgtttt ctctaaggtt ttatccagca gggcggcatc ttggggattt
    23821 tgcatcgcca attgtgtata attaataact ggcgtaagca ggttattcaa ttcgtgagct
    23881 gtcatggccc atgccatacc aagattggca agcggctgta gttgtgttag ctgctcttcg
    23941 agttttaggc actgattttc aaaatgagca atttgatgat gtagcgattt ccgattttca
    24001 agtaagtgcg ccaaaatgtc ctgctccatc agcaatctgc gataaataat ttttttagaa
    24061 atatctgcca aaataatatc ggcaattatc taaccttttc ttgaaaaaag acccgataaa
    24121 gataattcta atccatttgt aatgcatttt ttaaagaaag ctgctgggct ttgagaagta
    24181 cctgctgctt ctgtagtctg ccggcgagat tttattgaca aagaacggtt aaaacagtaa
    24241 catttgatat tataaataaa tccaaaaagg aaaatataga tgcctgacga taattatcgt
    24301 aaaccgagag ttttagttgt tgatgataat atccagaatc ttgaattgat tctggcctat
    24361 ctggaagacg tcgaatgtga aactctttct gcggaagggg ggcagcaggc attagatatc
    24421 atttacaatg atcctcctga tttagtcctt ttggatgtga tgatgccgaa aatcagcggt
    24481 ttcgaagtgt gtaaacgcat caaaaacaat cccaaaacgt ccgatattcc cgtaattatg
    24541 attaccgcat tgaatgaaat gggtgatatc gaacgggcca ttgactccgg cacagatgat
    24601 ttcctgagca aacctatcaa caaatgggag cttttgactc gagttaaaac gatgctgaaa
    24661 ttgaagcatc taacggataa attggaacgc actttggcct atttaaacga aatagagcaa
    24721 aatccgcttc agcagtcgta aactcccttc cattctagca tgttcgtgtc ttgaatcttc
    24781 ttttggcggc ttgtcaatcg ttatattcta tacaattgct ccaattagtg tattggttct
    24841 gttgccggcc aactgttttc ctgccgtgcc gattaattcg ctccaatgtc ctgtcgatgt
    24901 acttgtacag atgtatcagc aatatgagac gattttccca gtgcgacagg aattcattga
    24961 atgagcaagc tttttgagat ctttggaaaa ggaatcacaa tcagcacagc agatatcatt
    25021 tggcactggc tgggccaaat gctgcctcca gcggattcaa gcagtaacga tgtgtcattt
    25081 gtaaaaattc tggaccattt ggccaaccgc gagattgttc aggcagaaga gataatccgg
    25141 tcttgtctta taaaatctcc tgattgcgta tgggcccgca tggccgcttc ggccgtctgt
    25201 ttaatgcagg ataatattgc tgaatcgctt cagcatgccc aaagcgtcta tttccgccag
    25261 ccttccaata caatggctct gtatgtaatg ggctattgct gcgagcgtct gggacagcca
    25321 gaacaggcac tggaatttta tcaggactgt gtcaaattta aaaggcatct ccagctgccg
    25381 catcagcgga tggctgccat ttatctgcgg gacggaggca ttgataaggc ggcggcagaa
    25441 tacgagcaga ttacggctga gcattatgag gatatccaat cgctggtgct tttgggctat
    25501 ctctatcttg attctggaaa aatagagcag gctatcgatg ctcttaatct ggccatcgtt
    25561 tcgcatcctg ataattttat ggagtttcac gagcccgacg aaatcgaaac gcttatacag
    25621 gatgagatgt tcgaacaggc aatcgagagg atacgctgga caatcgaaca gatcgggacc
    25681 ttgccggatc tggtggtgcg gatggccgat atttacagtg catggggaaa agaagccgaa
    25741 gccttagcct gctatgagca tgctgttcat cttcagccca gctccttgga ggccgccata
    25801 aagctggcaa ctcattacct gcgcagccat cgtttttctc ttgctgcaga gcaattcaat
    25861 cgggctgcgg atataaatga agaaattgtc gatgcctata tgggattggc aatcgcccaa
    25921 gaaaccagcg accattctga agccgctttg gaaaccctgt cgctggcttc tgcaatactc
    25981 caaaacagta tattgctgta ctctgagtca gcatgtctgc agtttcagtc tgttatcgat
    26041 gaaacattta ctgacactgc aggcagaacc attgtttccg ccaatgatgt aatcaaagcg
    26101 tataaagaac agctcaaata cagcggcagc cgacccgatg tcaattacaa atacggcatc
    26161 ctgatgatcg gagaaaataa tctgccgctt gcatcctgtt tattcgaaaa aacattggat
    26221 attaatccaa tgtattatcg ggcacggcat aaattggccc tgtgtgcgtt tatggacggc
    26281 catccgcaaa aagctcttaa aatactgagg aacaatccct ccgcaggaaa aactgtattt
    26341 gagaaatatt atcagctctc gattttatac tccaacagaa aaaagttcag cgaagccatc
    26401 aggcagctga gtgttttaga aacatccgat atggcggatt tgtctcaaac acaggctaat
    26461 cttgaaatca ttcttgagaa tttggggctt atcgatagga ctatgaccag ctggcagcgg
    26521 ctgaacgaaa cctctgagtg tctggtaaaa atcaaagaga aaaaaccgat ttcaaatgtt
    26581 ctgtaagtct aactaaataa aaatgttgca tacatttaat caggcatttc cattcggtat
    26641 tgaatagccg ccaatgcaac ataagataca ttatgagacg ttacaaattg cgttttttta
    26701 gatgcagcta tcgggcggca ttccattacc ttgcaattgt ctctatcggt atcctattgc
    26761 ttggatttta aaatgcatct ctgaaagcca gcggcttatc cagaatttct tttaagatta
    26821 ccgaattccg cagattctcc ggcgagcgca ttgtttgcca gaggccgaca gtgaaaggtt
    26881 tgacaatttt atttttgtca gacaatatag ctgttatctt tttttcttct tgtgtaacag
    26941 cagatgatgt tttcgtaggg atcgatgctg gcggctttat aaaatattga ttgccagaac
    27001 cacctaccat ctggccgcca ataggagata caatacgtcc gataaagctc tcccgagcag
    27061 ggggcttata tgtctgactt ttctgctgtg caatgctttg tgcggaaaac cgaacctgcg
    27121 gtataaccgc ggctttttga cttttcttct gtgcaaggcg gcgatcccac tccgaaatct
    27181 gcctgcccct gtccttttta gggctggata atgcagatga tcggccggac ggctggtctt
    27241 gcgatttcgc acgatttgcc aaggccttgg ctatcgctcc tgcaagataa aacaaagcaa
    27301 tcacgataaa tgttgcaata tcgccccacc cattagctaa aaccagcaga ttattgctgc
    27361 tgttgtaatg aatcattttt cttcctctgg tttagatgcg gtgcctcgtg cgatggaatc
    27421 ccgcatggac gtatccgcat tgatgttctt cattctgtaa taatccataa tccccagatt
    27481 tcccttgcgg aaggcctccg caatagcttt gggaacttcc gcttctgcca gcacgacctt
    27541 ggcacgattt tcctctacca gcgcccgcat ttcctgttcg cgggcaacag ccattgcacg
    27601 gcgtttttct gcctcggctt tggcccgctt gaggtctgcc tcggcctgtg cagcctgaag
    27661 ctgggcaccg atgttttcac ccacatcgac atctgctata tcaatggaca gaatttcaaa
    27721 tgctgttcct gcatccaatc cttttgccag caccgagcgg gaaatattat ccggattttc
    27781 cagaacgctt ttatgggtaa tcgcgctgcc aatggtcgta acgatgcctt ccccaacacg
    27841 ggcaataatc gtttcctctg ttgcgcctcc gataagacgg tcaatattag cccgcaccgt
    27901 aacccgtgct ttggccttca gctggatgcc gtcttgggca actgcatcaa ctgtttcttt
    27961 gccttttcgc ggatcaggac aatcgatgac cttcggattg acgctggtct gaaccgcatc
    28021 cagtatatcg cggccggcca gatcgatagc ggtcgccgtt ttccacgaca gggcgatatt
    28081 cgccctgttg gcagcaatta gcgctcgaac cacattgggc acccgccccc ctgccaaata
    28141 gtgactctcc agctcacgct gggaaatatc caatcctgcc tgaaccgctg taatgcggct
    28201 taaaacaatt gttcgcggtt caacttttcg aagccacatc ccaatcagtt ccgaaaactt
    28261 tacatccgcc cgcgacaatt tcgcctgaag ccacaatttg aaaaaggcgg caataatcag
    28321 gataaatatc agcgctgtaa tcccgaccaa aacaaggatt gccaccaaaa taatgtccat
    28381 tgcccccgca gccagtaaac cgctcaattg ttccattgct tgtctccttt cgccggttta
    28441 tttgttcacc gcaaccacaa cacgcattcc ctcaacaaaa agcacccgaa cgggttcgtt
    28501 tttatcaatg aagccgcatt cggctgtaca ctgcacgcgc agcccatcaa aatcacaggt
    28561 gccgaccgga cgcaaaggcg tcacggtctt tccctgtcgg cctgttaatt tatccagcag
    28621 cgatttatcc ggtatggcat cgccctgctg acggttggga ggtgtcagca tcacgctgtg
    28681 tccaaaccgg gttttcggca gcagtttata agccaatacc aaaacgcccg gaatcagaaa
    28741 cattgctata atgacaccaa tccagcctgc tgttgaactg atacggaaga aaatcactcc
    28801 gccccctgcc gcacatgcca gtgcacaaat gctcagtaat cccccgctgg ggacaaaaac
    28861 ctctgcaacc agcaaaacag cacacagaat atataaaaca atggcaagga aaagccacca
    28921 gaacatcagc ttggctcctc gattttcttt acaacaatgc gattgcctga gacctgtact
    28981 acttcaatga cactggcctt gtcaataaac tcggcctgac aaactacatc ataaagcgtg
    29041 ccgccgatac gggcagtgcc tgccggccgc aagggggtaa ctgctgcccc cctgtcgccg
    29101 acagcaagac ggatgctgtc ctgctgaggt ggatttgctt ggagggattg cccaattgcc
    29161 ggcaccagaa taaatccgct caagaagccc agtttcggca tatattttgc caatacccac
    29221 gcaatcacca gaaatcctgc cacaccgccg ataattccca acacgccctg tgcaaaaagt
    29281 gtccacgcct cctctgtctg cggccacgga atttcgtccg gccggttttt gaccagcatt
    29341 gcaaaaaagc ccactaaaat acagacaatc cctgcaattc cggttatccc aaagccggga
    29401 atcacaaata tctcaagcag cagcagaata atgcccagaa ccagtatcgc cacttcaatc
    29461 cagttggcaa gacccgtcag gtacttgctg ccgactaaaa tcacaaggca gacaagtgct
    29521 gccagactgg gaagccccaa tcccggggta tggagttcga tgtagatgcc cagcagaata
    29581 cccaaaatta gaatacttac aacaacaggt gaattcagcc accgaacaag ctcctctgac
    29641 cacaaggttt caagacaaag aatgtctttt gaaaaaacaa ccccatcctg tttggctaag
    29701 aaatccagca tttcctgttc attctggaca actgcccgtg caatgccgta ttccaacgcc
    29761 ttttggtctg taagggtcag aagctcgcct tctttgacaa cgagtttttt gcccgcaagg
    29821 tcatacagat taggatccgt tggaagataa tctttctcaa aatactcgaa tgtccggctt
    29881 ttcttgtttt tcacctgcca gacctccagt ttttgggtca ccatcgcctt cagcagcgcc
    29941 tcgggatagc cgttggcttg ggctgcacgg ctgaaggcag cacggacaaa actctcagcc
    30001 ttctcacgct ccgcttcacc catctctgta tcgcccatga caataggcgc actgcagccg
    30061 attgtggtgt tctgccgcat aatgatatct tggcaggaaa cgcttatcat agcgcctgca
    30121 gagatggctt ccgtgctgac ataggcgact gtacgggctt ttttcgccaa atcaagaatc
    30181 aagtacttgc tgatgtcatc ggcacttttg accaagcccc cgtaagtttg gatatcaagg
    30241 atgatataat caacccccct gcgcaccgct tcttcacttc gccggacaac cgattgatag
    30301 aggccatggt caatcatctc ataacaggtc aggatggcag cggttttaag gggcttttcc
    30361 tgctcctgag gctgagcctg cagcagtgag gccccaataa agcaggcaaa ggccgccagg
    30421 atggcaaaga ccagccatct ggatattata gactctttct cacgcataac tgtatattgt
    30481 agccgtattt ttttcagaaa caacagcaaa aataccgtat aactaaacgc ctccgcttct
    30541 gtcctgtttc gaaaaaaaac cgctgtttaa tagacagcca tgcagcagaa caaagatggc
    30601 tatttttccc caaccagaac ttcgacaata ttaaatgcgt ggtctttaat gcggcggtat
    30661 gccgttagca tatccgtata aatcaaaccc agtaaaggat ttgtaactcc ttctcccacg
    30721 cgaaccagat accgggagcg gcattccttc atcaaattgg taatagccag tcccttggtc
    30781 tgcatttcac tgaaaaattc caagctgctg tcttcccgtt caattccttg acagattaaa
    30841 tcaaggtact ggtccacctt gtcgtggaga ctgataaggt cagcaagccc cccctcgctg
    30901 aatttctgct gggtgtctct cactttcaga cgcagtttca tcaagccctg tatgtaatcg
    30961 ctgattgact ccagctcatc ggctatccgc aattggccgc tggcttcctg cataagctca
    31021 tgggaaatgc tgccggtcat aatctcgccg ataaactcga cgatttcttt ttggataaca
    31081 tccaaatcat tttccttctg gaaaatttta gatgccagct ctcgattggg ttcgtcgttc
    31141 gacagaaaag atcggaaact cttcagcatc gactggcagg tttgcgccat ttttatcaat
    31201 tctttatagg actgctgaat cccaatggcc ggagcatcca gaatacgaat gttcaggtac
    31261 gtcagtttgg gtttttccag ggctttcttg tccggcacca gccacatcag aaaacgtgcc
    31321 aaaagaccga taaacggcat aaagagaatg gtatttacga tgttaaatcc ggaatgagtc
    31381 agggcgatag ccggacctgt aaggctgtat tttgttcctg cgggggtata aaggggcaaa
    31441 tcggctaacg tatgcttttg tacggccttt tcaaattctc cggccattac ataggaacta
    31501 ttttcaggca ggacatctcc cttcagattc acccgatatc cgtctggatg agtaaaatac
    31561 agcacctctt cagaaatgtt atattccttg gcgtactgct taattaaatt atcctcgttt
    31621 gtttccacca caacagcctt gagatgccga agctgcgttt tcgttgtaag ccatgacacc
    31681 atgttggtat aaggtgtgaa aattgctgta atccagcata cgccgatgac attaaaagcc
    31741 acatgagcat aagccgcccg gcgcgcattg gtggcggcac ccagcgaagc aagaacagct
    31801 gtaatggttg taccgatatt ctctcctaaa atcagtgcag cggctgttcg ataatcaatc
    31861 gttcctgtca gcgccaaagc catggtgatg cccaaagtcg ccgacgaaga ttgaacaatg
    31921 gccgtcaatg cagctcccac cagaacacat ttccaaacat caaagtaagt atccggatgg
    31981 aagcgatgaa accatgcgat gaatcccggc atttccttga tgggcgcaaa accctgcgtc
    32041 atcagctcca gcccgaagaa taccatcccc aatcccatca gaaacataaa gaagaaacgg
    32101 atagaatccc gcttggaaaa caaatagaaa aacgaagacg ctcccagcag tgggagcccg
    32161 tatttgctga ttttaatcac cagtatccag ccggtaattg tggtgccgat attggcccct
    32221 aaaatcactc cgatggcctg cagcaggttc ataatgctgg cgttgaccat gcctacaacc
    32281 attacggtgg taaccgaact ggactgaata atacaggtaa ccagcgttcc tacgccgcag
    32341 gcaagaaggc ggttattggt gacagcccca atcattcttc gcagccgttc accggctact
    32401 gcctgcattc cctctgacat gtttttcatg cccagaagga agatgccaag accgccgcac
    32461 acctgaaaaa taatttctaa tgcttgacta ataaaaactc cataacatgc ttgctgatac
    32521 tggagaaaga cacatttttg tggatttata ccttttcccc caataaaaat caacgagaat
    32581 aaaaaacttg cgacagtaaa aatgctttat cggctacagt ttatgaatcg gaaatacagt
    32641 ttgcttaacc tgctcaatga tctgattgac ggcgggttcc agattctgac gctgccaagc
    32701 agacggaatc agcacgcggt cccgcaggcc tgccccgccg gcccagtcca tattttccaa
    32761 cataactcct cccattccag cggtcatatc ctgtgccagc tgttcgccgc atagtgccgt
    32821 ccagatcagt gcccacgccc gcacagtatt ctggacgttg tatcctcccc cgccgacggc
    32881 aagaatcggt ttcccaaacg ccagcagact gttgataatg tcgacaaaaa cattattggt
    32941 cagacacaaa ccggccagcg gatctccctg cagggtatct gctccggctt ccaaaaccag
    33001 aacatccggc tggcgggcac tgattaaggg cacaacaacc tgatgaaagg ccttcaggta
    33061 aagctcatca taagttcccg gaggcatcgg aagattgacg gcgtatcccc tgccttcgcc
    33121 tgcaccaatt tcgtccgtaa atcctgttcc gggaaagagg gtccggccat cctgatgcag
    33181 ggaaatggtc atgacatcgc agcggtcgta aaaggccgcc tgcaccccgt cgcagtggtg
    33241 aacatcaatg tccagaaaaa ccaccttttt gccggcccct gccagttcca tgcagcccag
    33301 cacgacatcg ttgagataac agaatccgcc ggcgtggtca tcaaaggcat gatgataacc
    33361 ccctgacgga ttgaacgcca cggccgccgt gcctgcaata atcgcacggg cagctgtcag
    33421 ggtcgcccct gccgcccagg cagcataatc atacattcct gcaaatacag ggcagtcacc
    33481 tgttccgaga ttcatataga gcatttcata gtcataatcg cccttgtcag cccgctgaat
    33541 cgcctgcaga tactctttgg tatgaaagcg ggataattcc tcgaaggtgg ctttttgcgg
    33601 agcagcaaca gacttgttgg gtccggttaa aagccccata gaatcgagca tctttcggac
    33661 ttgtccggca cggcaggtct taaaggggca gctttggggg tatggatact gctctaattc
    33721 ggtacaatgg acaaaaatgt tctttttttc agattcggtc ataaatcggc cctcaaacag
    33781 aaagaattat atccatttcc ggccggtccg caaattaaat ctccgccggt accttgctga
    33841 atttggctta tttggcggtt tttgtgcttg cctcaaatcg caaattcgat tagacttctg
    33901 cttttcgtag aaatttaaga cgtagtttaa ggaaaattgg aggttatagg cagtggcgaa
    33961 tcttaaagac attcgtaata tcgtgcttct cgggcacggc aatgccggca agacctcact
    34021 tgttgaggcc attcttcata ctgcaggcgt caccacccgc ttaggcagcg tcgaggaaaa
    34081 gaacaccgtc agcgattacg acgacgagga aaaacagcga ggcaattcga tacattcctc
    34141 cctgatgtac ataaacttca atggaaccca cattaatctg attgacaccc ccggttatcc
    34201 ggattttgta agccctgccc ttgtgagcat tccggcagcc gaaaccgcac tgattgtcat
    34261 tagcgcctca gcgggcatcg aaatgaatac ccgcaagctg tttgccgctg caacgagtgc
    34321 aggcaaggca cgcgtgattg ttgttaataa aatggattcg gaaaatgctg atttaaccga
    34381 gcttgtcagc cagattcagg aatcatttgg acagcagtgc cggtgtgcga atttgccgtc
    34441 ggcagataag acttcggtga ttgactgcat cgccaacgag gcggggcagt cggctgttat
    34501 ggatgtggcc caagcccata cggagctgct ggaaagcgtc atcgaagccg atgatgccct
    34561 gatggaaagc tatttgggcg gcgaagcggt ttcgcctgaa aagattgcag atgtttttgt
    34621 taaggccgtg atggccggca cggttatccc gattgtgttt accaatgccc gcaaggaaat
    34681 cggcatcaag gaactgctga cgcttattgc caactgcaca ccctctccgg cggatgtgcc
    34741 tccggcagtc ctcaaacacg gcgacaagac aatcgaggtc aaggccgatc ccaatggtcc
    34801 gctggcgggt ttggtttttc gaatcggcta cgatccccgc tccaatatga aatacgcctc
    34861 tattcgtctg ttcagcggct cgctggacag cggcacgcag ctgtatatca acgatgccaa
    34921 aaagccggtg cggccgggac atcctctcaa aatgcagggc ggcgaggtta aagaggtcga
    34981 aaaaggtctc tgcggcgaca ttatcacgct ggccaagatt gatgaactca agcttggcga
    35041 taccgtgcac aatgggtcgt ttgagggcaa atttgctatg ccgcctgtgc ccaaaccgat
    35101 gtttgcatta gcactggagc cggtagcccg cggcgatgaa aataaaatca gcgccgctct
    35161 ggaaaaactg gccgatgaag acgcatgctt caaggttacc cgtgacacgc agacgaagga
    35221 attggttatc aatggtttgg gagatctgca tctgcgggtg atgctcagca agctggaaaa
    35281 ccgctataag gttcatgtca acaccaagcc cccgaaaatc ccctaccgcg agaccattac
    35341 cggcaaggcg gaaggacact accgccacaa gaagcaaacc ggcggggcag gccagttcgg
    35401 cgaagtgtat ctgcgggttg agccggcgcc gcgagactct gacccatcgc tgatttattc
    35461 atgggatatc ttcggtgggt ccatcccggg ccagtttgag ccggccattc tcaaaggcat
    35521 taacgatgtg atggaaaacg gctttttggc gggttttccg atgcaggata tcaaagtgtc
    35581 gatatacgac ggaaagtacc accccgttga ttcgaaggaa gaggccttcc gcacggctgg
    35641 caagggcgcg tttctggatg cccttgaaaa agccaagccc accctgctgg aaccgattgt
    35701 caatatcgaa attaccgtgc cggcggaaaa tatgggcgac atcacaggcg atttggcctc
    35761 gcggcgcgga cgcgtcagcg gtcaggagat gctgccgggc gggatgatgg ttgtcaaggc
    35821 acaggtgccg ctggcagagg ttgccaacta caacagccag ctgaaaagcg ttaccggcgg
    35881 tcagggcagc tattcgatgg aattgagcca ttatgagccg acgccgccca atatccagca
    35941 gcaggtcatc gaacagtaca agaaagaaaa gcaggaggag aaggaataag cggccgaaaa
    36001 taatacaagc acggtttggt agcgaaccgg gcttttttat cggctcacac ttgttctgcc
    36061 agctcggcct gaacgccgat tctactgctg gtgcgatcga gaaagtccag cgtctgtccg
    36121 gtcagccggc gtcttagttg aagttatctc ggtgctattg ctgtcggccg gccagatagc
    36181 ggttgatggc ggcggcagca atgcgacctt gccccattgc cagaatgacg gtggcagccc
    36241 cgagaacaat atcgccgccg gcgtacacac cttccattga ggttttgcag ttttcatcga
    36301 caacaatatt accccatttg ttgaactcca gccccggcgt tgtttgctgg ataagcggat
    36361 tggcttcatt gccaatggca acaatgacgg tatcgacgtc cagcataaac tcgctgccct
    36421 tgatgggcac agggcggcgc cggccggatt catccggctc gcccaattcg taccggagac
    36481 attcgatgcc agccacatgg ctgtctgaat tgccgagaat tcgtttggga ttctgcaggg
    36541 tgcaaaactg cacgccttct tctttggcgt gatgaacctc ttccacacgg gcaggcattt
    36601 ctttttcgct gcggcgatag accagataga cattttgcgc ccccaagcgc actgccattc
    36661 gggcggcatc cattgctaca ttgccgccgc cgagcaccgc gacatttttg ctgacagcaa
    36721 tcggggtatc ggcccccctg ccgaaagcat acgctttcat cagattagcc cgggtcagat
    36781 attcgttagc gctgtaaacg ccgatcagat gttcgccctc aatgttcata aatttcggca
    36841 agccggcacc tacaccgata taaacagcgt caaagccgtc ttcggtcatc aattgctcta
    36901 tcgtgcgggt gcgtccgaca ataaaattgc agacaatctg gacgcccatt ttctttaata
    36961 catcgatttc tttctgcacg atggctttgg ggaggcgaaa ttcgggtatg ccgtaaacca
    37021 ttacgccacc gggcttatgg aatgcctcaa agacagtaac atcgtggccg gcccggcggg
    37081 tatccgcagc ggctacgatg ccgccgggtc cagagccgat gactgccact ttcctgccgg
    37141 tagcagaagc aaccgccgga acagaatctt gccggctgca aaagtcagct gcaaaccgtt
    37201 ctaaacggcc gatggatacg cttttgctga catctttgaa cttcaggcct acggtacatt
    37261 tttcctgaca ctggacttcc tgcgggcaga cgcggccgca gacagccggc aggagggaat
    37321 tttgcttgat gatggaaagc gcttcttcaa atctgccgtc tgctatagct gccacaaact
    37381 gcggaatgcg gattcgcact ggacagccgt caatgcaggg tgcattcttg cattgcaggc
    37441 agcgcagcgc ttccagccgg gcctgcatag ggctatagcc caaggcgacc tcgttcatat
    37501 tgcttcgccg ccggacagca tcttgcgagg gcatatcctg cgggggaata gcaaagcggt
    37561 ctttgggttt caacaagcca gctttatgtt tttctactaa ttcctgaaat tctttctccg
    37621 tcacgctgaa cctctcttga ttctttccaa tgtcccattc atcacggggt tgttttggga
    37681 tttaagccga ttttacagac atgggtgcgg aattcctcgt aggccttctg ttcgagactt
    37741 ttgtaggcgc tcatccgctt tatcatgagg tcgaaattga cttgatggcc atcgaattca
    37801 ggcccatcca cacagacaaa tttggtctgc ccccccactt cgacgcggca gccgccgcac
    37861 atgcctgttc cgtccaccat aatcgtattg agggacacct gcgtcgggat gccatattct
    37921 tttgttgttt gacagcagaa tttcatcatc acggcaggcc caatggcgac agccaagtcg
    37981 ggtttcggct cccgccggca gacctcttgg agcggctcgg taacaagcgc ctttcggcct
    38041 ttggaaccgt catctgtaac aataatcaat tcgttgctga cggctttgaa ttcctcttcc
    38101 agaatcagca gttctttgga gcgtgccccg atgatgctga caagacgatt gcctgcctct
    38161 ttgagagcag cggcaatagg cagaagcgga gccgcaccga ttccgccgcc gacacatacg
    38221 actgtgccaa atttgtctat gtgggtggga cgacccagag gaccagctac ataagccagc
    38281 gaatccccct gctgcaattg agccatttgt gcagtggctt tgccgacacg ctggaaaatc
    38341 agccggattg tgcctctgga ggcatctgct ccggcaatgg tcagagggat gcgttcgcct
    38401 gcatcggtat cagctgaaac gatgacaaac tgccctgctt ttcgtgcacg ggccaccata
    38461 ggcgcttcca cgtcaaactg aaaaacattt tccgataatt gccttttgct tacaatctta
    38521 aaagacactg cagggttcct tttgtcgtat tcaagataat attttaagat aaatgaataa
    38581 gttttactta ttaacagcaa ccagcaaagt tttcaagtat tttctgccga ttcaaaacag
    38641 attttgaatg cttttcattt ttgctgtgcg gaaatgattt tcactatcag ccgctccatg
    38701 gcagaagaag ctgttgtgcg gcctgttttc atctcatagt caatgcgacc taattctgct
    38761 aagatgctgg ccagctgcgg cagcgagagt cggtcggctt gggcaagcag atcgtccggt
    38821 ctgcggctgt ctacgttttt tgctgcctgt atcgggggtg ttccgcggct catcagggcc
    38881 tttaccctga aaagacggcg aaaatggtat ccaaacgccc ctattacagt gtattccgag
    38941 tccctgtcgg cggcaaacat ccgccgcagc cggctgatgg catcttctgt ccggcctgta
    39001 ctgacggcgt cgatgacatc gaatgcgtcg aacaggcggt tgtgaccgat taaggcttcg
    39061 atatccttta aggaaatagt tttggacggc gctgcataag cggccagctt atctatttct
    39121 ctgcataagc ggccggggtc atcgcccgtc agctcaacca gccagcggct acattggctg
    39181 tccagccgga tgccataggt ctgctgggca taattagaaa cgaatgcagg aagctggtac
    39241 gtcttgatgc cgggtatttc gataacggca ttgagtttct gaagcttttt gtgcagccgc
    39301 gtgcgtttgt cccaagaggt ggcggttaga atgagcacgc tggagggact gggattctct
    39361 atgtactctt ccagagcttc aaggttggac ctaataaacg attctgcatt tttgataaca
    39421 acaattcgtt tggaggccaa aaacggcagg gtccacaatt cttccagaat gtccgcaatt
    39481 ctggcttcct cagctcgcgg ctcatacagc gccatttcgc gttcgttttg atccagcaat
    39541 gaatccagaa gccgctggca ttcgacactg acgacatagg gatcgtcgcc gcaaagcacg
    39601 ataaaaggcc gggatgtttc tgttgtcttt tttgacacgg gatttctgtc cggctacggt
    39661 ttggattggt cgtcctgaat gtcctgctgg tcctcttcta caatgagccg ctcggctgca
    39721 acaagcttgt cgccttcctt gagcgaaatc attctgacgc cggcagtgtt gcggccgata
    39781 gcccgaactt cttcaagacc tgttcggacg atcatcccgt tggctgtaat catcatgatt
    39841 tcgtcatcgt cgtgaaccgc tttgatggct acaacctttc cgttgcgttc ggttgtcttg
    39901 atgttgatca ggccgatccc gccgcgtccc tgcacccgat aatccgacaa ttcggtgcgt
    39961 ttgccatagc cgttttcaca gacggtcagc aaaaaggctc cttcttctgc tataaccatg
    40021 ccgataaccc ggtcctcgtc ccgcagggta atgccacgta ccccgcggct gacgcgtccc
    40081 atcgggcgga catccgtctc atggaagcgg atcgccattc cattgcgcgt gccgagaatg
    40141 atgtcgtcgc tgccggaagt gatagcggcc cctatcaaga catctccctc atccagcttg
    40201 atggcaataa cgccgctgct gcgcggattg ctgtaggcac tgagggctgt ttttttgacg
    40261 atgccgttct gggtagccat aacaagctgc cgcttgtcgt caaattcgcg aacattgata
    40321 atcgatgtaa ttttctcgtc tttgtccagc tggagaagat taatgatatt gcgtcctttg
    40381 ctttgtcggg acatggcagg cacattgtaa accttcagcc agtggcacat gcctttgctg
    40441 gtgaaaaaca tcagcgtatc gtgcgtggat gccacaaaca gatgctccag aaaatcccct
    40501 tctttggtgt ccgagccgat gacacctttg ccgccccgcc cctgtttgcg ataggcatca
    40561 atttcagttc gcttgatata gccggcgtgg gtaattgtca caagcgtttc ctcgtcggca
    40621 atcaggtctt caatctccag cgtatcggcc tcgtccatga caatctgcgt gcggcgcggg
    40681 cttttgtatt tttccttgat ttcaaaaagg tcctcccgaa ttacatccag ctgcaaatcc
    40741 ctgctggcca gaagagcttc aaagccggcg attttctcgg ctacatccgc atattctttg
    40801 gccagctttt caatttccaa accggtcagc cgctgcagct gcattgtcaa aatagcatct
    40861 gcctgagggc cggacagaaa gtgatcattt tttgtccgaa ggtcaacaaa ttccgccgga
    40921 agcagtttac gcagggtctg gctttctgac aggcgaaggg gttttttcat caggttgatt
    40981 tttgccgtag gcccatcggg tgaggcttta atcagggcaa taatttcatc tatatcgctg
    41041 acggctaaaa tcagcccttc caaaatatgg gcgcggttgc ggaaacgccg cagcaaatgc
    41101 cgtgtccgcc ggcggattac cgtaagtcga tggttaatat acagccgcag catctgcttg
    41161 atattcagag tttccgggcg gttgttgacc aaagcgacat tgttaacggc aaatgttgtc
    41221 tgcaaaggag taaaacggta taatttgttc attactacat tgctgtcggc atcttttttc
    41281 agttcgacaa caatgcgaag gcctttgcgg tctgactcat cacggatatc ggagatttca
    41341 tcaatctggc cgttctggac gcattcggca atttttgcca cgatgttgct ctttacggcc
    41401 atgtagggaa tttcggtaat aattatgctt tccttgcccc gcttgtttgt ttcgacatgt
    41461 gccttgcagc gtacgcgcag atgccccctg ccggtaacat aggcatccat aatgcccttg
    41521 cggccgcaaa taataccgcc ggtcggaaaa tccggccccg gcaggcgttc aagaatatcc
    41581 ttaaagccgc aatcggggtc ctctatcatc agcaggagcg catcgcatac ttcttctaca
    41641 ttatgcggag ggatgttggt tgccatcccg acagcgatac cgctggcacc gttgacaagg
    41701 agatttggaa acttggccgg cagaacggtg ggctcctggc gtgtttcgtc atagtttggg
    41761 acaaagtcaa cagtatccag cttcaaatct tccagcatgt ccacggcggc ctgcgtcatg
    41821 cgggcttctg tgtaccgcat cgcagcgggc gggtctgcat caatgctgcc gaaattgccc
    41881 tgcggatcca ccagcatcac ccgcatattc cactcctgcc ccatacggac aagggtgccg
    41941 taggttgcct gatcgccgtg agggtgatag ttgccgcttg tatcacctac aatcttggca
    42001 cattttcgat gtttggagcg ggggccgagg ttcaaatcgt tcatcgctac taatatacgc
    42061 cgctgggagg gcttgaggcc gtcgcgcaca tccggcaatg cacgcgcaac aatgacgctc
    42121 atcgcataat tcagatacga attcttgagt tcatcaagaa tctgcatatc ctcaatatgt
    42181 tcagtgtact ccggatgaac cgtattttcg tccataaata tgcctttaga ctgttttaga
    42241 aagataaaac cttatactgt gttttattac atattgcaaa ataataagaa taccttaatt
    42301 cggtcttgct gtcaatcttg ccccaaatcc ggcaggccct tttttaagca tattttcagg
    42361 gttccataca gaatttgctg tattgcggcg gccttcatag tatggggata taaagcggct
    42421 gctgtgccgg aaaccagacg tcagtaaagc tgctctgacc cgccgaaaat agccttgatt
    42481 gtatccagat tggggattat gggcggcagg ttatccgccg gcgagaaggc ctgcgaccaa
    42541 tataccagta tggccttgcc catcagataa tcacgcggca caacaccggc atcgtatgta
    42601 cggcccctgt tgccgatacc ctcattagac cagaggcgtc catctaaact attgccggta
    42661 ttgtctccgc agacaaaata ctgctcccta cccagcacaa atggaatttc atctgttgcc
    42721 cgcagagaat acccttcttt gcccatataa aaaatatcgc ggaagagtcc tacatgcctt
    42781 atttgaaccg gaccggctgc aaagatctgc acgcggggcg gggtttcaga actggccggc
    42841 tgcggagaga aatcggggtc tgccgaaagg tcataggtga gccgccggct gccccactgc
    42901 aataccagaa gctggtctac acaggcaaat tcaaacttct cataccgccc cggggcaatc
    42961 ggtcctatca gcatcggtaa gcgcaagtct tttgttgtat cattgacggt tttggttaaa
    43021 accataactc ccccgaattc gatgcgtgcg gagtagagaa cattgtattt ttcaagggaa
    43081 gccccaatac agccgccgct ggcttcatgt tgggcataag atcactgaca atgggtttgg
    43141 attctttatc ccatccgtcg ttgtaggcac agccggcgcg gaagcaagaa ggatcattgg
    43201 ggacaaagtt tatcgtatga agctgcccgg atgtatcctc cagagaaaaa aacgcacgct
    43261 gaagctgcca agtcgaattc ggttcatttt gaaaaggcag cggactgggt ttgttaagcg
    43321 gacacatagg gtcgtcattg ttcacaagag gcggattgtc ctgcagaaaa atgggcgtcc
    43381 acaactcttt ttgcacgccg gctggtttgc gggctatttg accgttaatg aaaacatcac
    43441 cgttgatcag ctgtactgtc tcgttgggca gcgcaatcag ccgtttgatg taattgtcct
    43501 tgggattgat cggatttttg aaaacgacga catcccagcg tttcggttca aaaaactgat
    43561 aaatcgattt caaaacgaat atgcggtcgc cgtttttcat attgtctgcg gtctgggggg
    43621 gctggctgca attgcagatt gggcagcgcg gaggcagaaa ccctgccgta tcgctgccga
    43681 tatcaaaggc atatccgcac tgctgacagc gcagatgata atgagctccg cggagggtat
    43741 ctgccatgct gccggtaggt atctgaaatg cctgaacagc aaaaccctga aacagcagcg
    43801 ccaagataaa cgcaaccagc agccattcga aaagggtaac aatgttttca acagaatgct
    43861 gatgccgatg agccaagctt tgatagggct ggttttgttc cattatacaa taagagattt
    43921 aaaacatctt aactcctgtt gatacggaat tattccgtgc cgccgtaaat aaccttcatc
    43981 ccatccacaa aaggaatcca gcgaatcggt atccggctga gaggtccttt ctcaatgcgg
    44041 tagccgccgg gccagtgcac gacaaatgcc tttccgacaa gatagtcttg cggcactatg
    44101 ccggccggat actcaggtcg gcccggattg gccagacccg ggcggtccca cagtcgggaa
    44161 tccagactga atggactgtt gtcgccgcag gcaaagtatt caccgtcatg catgaccatc
    44221 ggggactctt tggtcccgcg tttgatattg tctgaaatat agtggatgtc tctgtataag
    44281 gcaatgtgac tcagacgaag gctgccgatg ccgaaaatct gaacaagggg cctgatatga
    44341 taatccgtgc cggcatcttc aatgccgttt ccgaggtcat atgcaacctt cgaacttccg
    44401 tactccagaa tcagctgatg atccaaggtg gcaaaacgaa atcgggtaat ccttttcaaa
    44461 tcgtcgggtc ggcattgtcc ggctgctatt ataacaggtt cccctgctgg gcttaatcgg
    44521 gcaatctcca tccggccgtc ggaatgaatc cagccttcat aggcgatgcc gtattttcga
    44581 atctgtgctc ctgcggccga gttttgcccg atagaggcat aatattcggc catcaaatcg
    44641 ctgcacaaag gcatcatcgg ataactggcc ggatcatcat aggcatatgt ggcgcgaaaa
    44701 tcattgccct gcctgtcatt gtattgaatg cggtgaattg ccgaatccga acttttcagt
    44761 tcgaacaccg tagggccgtc ggctgataaa ttccattggg atgcttctac acactcgaac
    44821 ggctgtctcc acgtgtgtcc attaaaactc ttttcatcag gacgtgccgg aggaaagtcg
    44881 ttatgataaa ccaccatcca tagttcttcc tgaacattag gcggttttcg ctgaatgagg
    44941 ccgttgatgt aaatgtcgcc atcaataatc tgaagggtct cccccggcaa ggcaatgcag
    45001 cgtttgatat agttaatgcg gggttcaagg ggatttttaa aaactataac atcccatcgt
    45061 ttcggctgga aaaactgata gatgcatttg atgacgaaaa tctgatcacc tttgaaaacc
    45121 gtccgcgcaa aagcaggaac cgttttgccg tctttataga taaaatagcg gccgtcgctg
    45181 gaacggattg gggccggctc ggcatagccg cagcttggac agcgggatga gcggggacca
    45241 atgggaagtt tttgggatgg ggttatggtg tcagacatgc cgtatcgctg tgcaataaag
    45301 tcgtggtcgt agcgaaatcc gcactggccg cagcgaatcc gaaaatgcgc accccgaaga
    45361 gtttcggcca tactgccggt tggaatgcgg taaacctgca taacaaaaat gataaagaca
    45421 agggtgtttg caaaagcggt aatcagccac tctgcattac gatatgcgct gtcaattttg
    45481 tctttcccag agaaaacaag attatcatta aatattgaag acatattagt tccgcttttg
    45541 tgataaaaat aatccttcta ttttatacat aatctaataa aggggcaaga attgattctg
    45601 tcattaggaa aaaaatccaa gtttccaaga ctgaattcca taacttaaaa aaaaatctaa
    45661 aaaaatcgct tgatgattag aagtcttttc tatatagtaa gttgcaataa cgcaaatcag
    45721 cttccatgag accttgcggc ggattaaaaa ttttaaagaa taaaataaat agttaaaaca
    45781 agagggtcgc agatgtcaac aatcacgaag aaagatctaa ttgacagaat ttctgatgcc
    45841 acacaggcaa aaagggttgt tgtaaagcgc attattcagt cctttcttga tgagattata
    45901 tctgaattgg ctgcagataa ccggctggag tttcgcgatt tcggtgtttt tgaaacacga
    45961 agccggacgg ctcgtgtagc gcaaaatcct aaaacactcg agcgggttaa ggtgccatcc
    46021 aaacgcagcg tcaaattcaa gatgggtcgc ctcctgaaag aacgtttgtc tggcagttct
    46081 tcgcttgctc ctacgcggga tgcggattag accttctttt atgcttgccc attttggccg
    46141 gcacaagtaa agtaagtatg caggagacaa aaatggccga aggaaaggtc aaatggttcg
    46201 ataaaaagaa gggatacggt ttcattctca gcgaagaagg caaggatatt tttgtccatt
    46261 ataccagctt tgcagacagt tctttgcgtt ccctgaacga tggagacatc gttgtatttg
    46321 acatcgttcc gggggaaaag ggtctgcgag caaagaatgt ttctctgaaa acagctgcga
    46381 aagcgacagc agattcaggg ttatagcttc agaagaatag ctcctgtcgg attggactat
    46441 atctgtgaga gcagtgttat cggaagatag aagtgccgcc tacgggtgag atctatacgg
    46501 cggagggtct ggtttagtaa aagagcagaa aggatgtcca caataacata ccatgaagct
    46561 ctggactgcc gggtgcttgt attgaataag cactatttgg cactgcgggt tatcggtgcc
    46621 cggcgggctt tttcgctgtt attccgcaat ctggctgaag ttgtatcttg tgatgacggg
    46681 acttatgcca attataattt tcaaagctgg atggaagtca gcgaagcaaa acgcacattc
    46741 gaagcccaca agtacgactg gataaggacg gtcaattttg atatcgctgt tccacgtgtc
    46801 atccgtctgc tctgttatga ccgcattcca aataaaagcg tcaaactcaa ccgccgcaat
    46861 atatttgcac gggacagctg ccattgccag tactgcggca agaaatttcc gccggcggaa
    46921 ctttcactgg atcatgtgat accgcgccgt ctcggggggc agacaacatg ggaaaatatt
    46981 gtctgtgcat gtatgaaatg caatgtcaaa aaaggtggcc gcacgcctgc tcaggcggga
    47041 atgaagctgg ttcagctgcc ggaaaaacca aaacggaatc ccgttattca tatccattta
    47101 agccatgaac gctacagcag ctggaaacaa ttccttgaac atgcttactg gtcagtggaa
    47161 ttgacttaaa agattgaagt tatatcagaa atggggatga aataatcctg ctgcaagatt
    47221 ataaaggccg tggagcgcca tggctgcagc aaaaaaaaca aaaccatgtg acaggtcagc
    47281 tgccctgctg tctttgagct gtattttcca aacacggatg agccctaaag aagcaatcag
    47341 cgaacaaaca agatgaagca aggtacatac ggtccagcga tacaggctgt actgatgcgg
    47401 attattcaaa gagcttatcg gtatatagag ccagatataa agaaggtttt cggacgcaga
    47461 gaaaatgaat gcagaaactg ctgcggcaat aagaaactgt cctgctgaaa aaatgcgata
    47521 cggctttttt tccaaaaggt atatcattcc tgattgctta agcagttctt caatgatagg
    47581 ggcaaaaact gtcatataca gggcatgtgt cagcccttgc ccacccgaaa taaccgctcc
    47641 caagacggca aaagagccgc cggtcagtgc ggcagccaaa gtgactgccc aattgccgag
    47701 ccgggtacac tggctgcgtt tttgagccag ccactgccct aactcggcct gcttcagctg
    47761 gcctgtgaag acaggttcat ttaaaaccga atcccgtacc gtcgagatct catcttctcg
    47821 gccgtctaat gggggcacgg cagattcact gggatccgaa ttgaagggac cggggtaatt
    47881 aaccggctcg acattgacat caaaataatc gggggaagca ctcatttcaa ggcggttccg
    47941 taagcgagca tttctacgcc gccgggtttg tttttctgct gtccttgaat agcagaagtt
    48001 tcgtagcgga tattccaaac aatacgcgcc ccctgctgct ttgcctgctc gagcatcctc
    48061 actgtcgcct gtcggcgtgc ccgctccata agggttacgt agcttttcat ttccccgccg
    48121 aataaggttc gaagccctgc cgcaaacacc ttaaaatagt ccgttgcgat aacggccgct
    48181 cccatcacaa gaaacggctg ggtgttttcc agttccggcg ggatgctttt caggttggtt
    48241 atcatcatat ctttatagca ttcttcctgc tgccggagtt gtctgaaatg gtgcagttcc
    48301 gtgccctttc caatcagcat aaaaacaaca accagtatca atggaattgc aatttgcagc
    48361 agaagttcta tcattgcccc tctcctttct gctgaacggc agcagacgga gttatcggct
    48421 gtgctacaac ggccgtgcca tagacataca attcggccgc accggaggta attgaactgg
    48481 tagcaaaacg gacattgaca acggcatttg ctccaagctg ctgtgcctga gccagcattc
    48541 gggccatggc ttcccgacgg gattccacca gcagttcggt atagccagtc agttcgcctc
    48601 cgactagatt tttcagcgag gcggctatat ctcttccgat atgcttggcc cgtacggtat
    48661 ttccttgtac aagaccttta atttgcagga ttttacagcc ggggatagtt tcggtgttca
    48721 cgacaatcat aaccactcct tgaaaatggg atacggcatc atattaaaaa actaaccata
    48781 ctatacccac aatggttttt ctaagcaata aaaaaacagc atccttatta tgagcagaaa
    48841 gatattcatg atatgcagga aaatgatgat taacagaaat tgaaccggtt attatctatc
    48901 tactaataac aaataaagtt aaaattccta ttgcaaatat tttctatcta tttataatgc
    48961 cttgtttgct cgatatttta gcttgttgga taaaaaatag ttattagttc ttcggagaat
    49021 agaaacgtaa atcctgaacc attaaggaaa tcaaggagcc gcaaggatat ggtatcttta
    49081 accaacgcac cagtcaatct gtttggggcg gaactgccga gttttgacca gataaaaaaa
    49141 ctcgggcaat ttgtcaatgc cagcgaagca atccggctga aattcaggca ggaagcggac
    49201 aaacaaagtg atgttttgat acaaggaatt gccctgctgc tgtgcggaca agcaggcgaa
    49261 gctattgaga aattaaacaa aggcaaagag tgtccgcaga aacatcttgc attgggatat
    49321 gcctaccgca aggtaaaact gtatgataag gcgattgaac aatttgacaa agccgccaaa
    49381 cagactgaag gaatgacgat agccatggaa aaagcagaaa cctggcgtct ggccggccag
    49441 ctggacaaat gcgaagcaga attgaaaaac tgtgccaact ttgaaaatgt cagcgctgaa
    49501 tatcattatc agctcggacg gctgctggat gcccgcgggg actacgaagg agctatcggc
    49561 cagtacgagc gtgcagttga actggacaac cgtcatatca atgccctttt ccatcttgca
    49621 tatgcgtttg accttcgggg tgatgacgaa gcggcggtga attattacaa ggccttgata
    49681 cagattgagc cggctcatgt gaacggactg ctgaatctgg cggtgctgta tgaagatatg
    49741 ggtgaatacg agaaagccat gcggtgcgtt gatactgttt tacgttcgca tccaaaccac
    49801 tccaaagcac agctgttccg caaggatatt gccagctcca gtgtcatgat ttatgacgaa
    49861 gaaaaggaaa aaagacggga tcggcagaac aaaatactgg aaataccgat ttccgatttt
    49921 gaactttcgg ttcgcagccg caattgcctg aagaaaatga atattgtaac actgggtgat
    49981 ttgctgcgaa ctaccgagca ggaactgctg tcgtacaaga atttcggtga aaccagtctg
    50041 atggagatta agaagattct ggaatacaag gggcttcgtc tgggaatgtc actggaagag
    50101 gaaggtccgg ccaaacctgt tcctgctgaa ttcactggtc aggccagtcc ggagatactg
    50161 aacaagtcaa cagatgaact ggaactgtcg gttcgttccc gtcgtgccct cgagcggctg
    50221 ggtgtcaaaa cggtatttga cttgattaac aaaacagagg cggaactgct tggctgcaag
    50281 aacttcggcg tgaccagtct gaatgagata aaagaccgtc tggccggatt gggattaagc
    50341 cttcgcaagc ttgagtaagg tacgccctta cagcaatgaa ttgtgttatg aaaggaatac
    50401 gcaggctcac ggcgtatcga agtgctgcgc tgtgggcctg tatggtatgt atgctgctgc
    50461 cggccggatg ccggcagcgc agcccggaac ctactgtcgg gacagagcgt ccgatacagg
    50521 aatggatgcg ggttttgctc tttggcaatc taagagagtg ttccgtcgca tcgctaagcg
    50581 gttttcgcat tgaagatatt gacggcggaa gcagcgctga atttatgacc tcagccccga
    50641 tacaaatccg gcatcaggat ggatttatcc aagtcggcga ccattttttc gggcggcaaa
    50701 ttttaattaa acccaacgcc ccctatgttg tttttgaaat cgacggcaaa gcctatcgcg
    50761 gctatcttcg cctccacatc cgaatggatg aagccggcat ggaagtcatc aatgaagtac
    50821 cactggaatc atatcttttc ggcgtggtag gagctgaaat gcagagctat tgggagccgg
    50881 aggcactgaa agcacaggcg gttgtttcgc gaacctacgg tttgtacatc aaaagccgct
    50941 ttggagccct gcgttcatgg gacttaacac agtccgaggc caaccaggtt tatcgcggga
    51001 tcgaagccga aacaccttct gtcagacagg cggtgttgga aacggccgga caaattctca
    51061 tcggcaagtg ggctgacggc agtgaatcgc tgtttccggc ttattacagc agttcctgtg
    51121 gtggacatac cgaggcggca caaaatgttt tcggaggatc tgcagcagtt cctttatcgg
    51181 gagtgctgtg cccctactgc aaggagattg cccgccggaa agattttcac tggcccaatg
    51241 ttacgcttac aaaacgtcag attttcgagg gattgacccg gcgctacgag tccttggaaa
    51301 agctggaagg cattgaagat tttgagattt cgcgtgtcgg ttttctggga cgtgttaccc
    51361 aagtcaggct gctcggcaaa aacggcaaaa ccgatactct tcgaggcgag gattttcggc
    51421 tgagtcttga tccgacaggg cgtaaaatta aaagtgctgt gtttaccgtt gataaaaatg
    51481 cagattcagt cgtttttcgg gatggtttgg gcttcggaca tggagttggt ttatgccagt
    51541 gcggggcgca gggaatggca cggcaagggt atacttatca ggaaattttg gatttttatt
    51601 tcccgggttc caaacaggtt gcaatagaga catcaaaaga gtaatgaaca gcttctgtgt
    51661 tcaaaacaag gatttgcata gtcaagcccg atgcggatgc ttgacaaccg ctcatgggac
    51721 ggttcagacc cctgcgttta tgcctgtggg aacacgcggc tgtgtgaaag gcattactcc
    51781 cgaacagctt aaagccaccg gcgctgaaat tgtgctggcc aatacgtatc acctcctgat
    51841 acggccggga cctgatgttg tcgaggcact gggagatctg catgggttga tgggctggtc
    51901 cggccctatt ctgaccgaca gcggcgggta tcaggttttt tcgctgagta ccttaaatcg
    51961 catcggggag gacggggttg agtttgccag tcatatcgac ggggcaaaaa tttatctgga
    52021 tgcccgcatt gctacccaag cccaaaaccg gctgggagca gatattatta tgtgttttga
    52081 tgagtgtacc ccttggccct gccctccgga acggttaaag aaagcggttc agcggactat
    52141 acgctgggcc cggcaatgca aagaggctca cactaattcc cgacaaatgc tgtttggtat
    52201 tatacagggc ggagttgacc tggaattaag aagcttgtgt gcggctgagt tggtgcccat
    52261 ggatttcgat ggctatgcaa taggcggatt aagcgtcggt gagggacacg attttatggt
    52321 acagacagtt cgtcatactg ccggactgct cccggaacaa aaaccccgct atttgatggg
    52381 agtcggattg ccgattgata ttgtagagtc tgttcgggca ggtattgata tgtttgattg
    52441 tgttttgccc acccgcaacg gacgcaatgc ctatgccttt accgacaatg ggccggttcg
    52501 cttgcgaaac agccgttatg tgactgatac ggagccgctg gagccgggat gcagatgcta
    52561 tacctgtcag catttcggca aggggacgtt gcggcatttc tttaatgtgg gtgaaatgct
    52621 gggtccgatt ttagtatcga ttcataattt aacatattat cagcgtttga tgagccgtat
    52681 tcgctccgag attaataatg gctcttttca agcttgggct gatgattttt gtataaaaca
    52741 caaaaacgaa atagacgaaa taaacgaaag ttaacatgat tgaacaagga gattaggatg
    52801 cacaatatct ggatattggc ttcttctgaa cctgaatcta aagaagaggg tcagattgta
    52861 acgatggaag agtcggcatc gaaccagcag acagctacat cacaagacgg ccggaatgga
    52921 gcagaccagc agagtcctcc tatgtcccag aaaccgaata cgatgtttct tgtgatgatg
    52981 gccattctgc tggtttatat gttctttgtg atgttccgcg gtcccaagaa aaaacagcag
    53041 gaacatcagc gaatggtcca gtctttgaag aaaaacgacc gtgtgcgaac aatcggcggc
    53101 attttcggga ccgttatgga tgtgcgggat gatgagattg tgctgaaaat cgacgagtcc
    53161 accaatacca aaatgcgggt cagcccggca gcaattgcag cagtgctaaa agacaagaca
    53221 gaataaaacg gcaacaacgc aaattaggca catattcagg aaccagttat ggacaaaaat
    53281 ttgatctgga agagcgtatt aatttttgtt gttgtggctt tggcagcatg gaatctgtat
    53341 ccgccgcagg aaaagctgaa accgggtatt gaccttgccg gcgggaccag cttgatttat
    53401 gaaatcgata cgacagatct ggcacccggt gaacagcgga atctggcaca aaatatgatt
    53461 cccatcctgc tcaagcggat tgaccccacg catgtagcca acgtcgttat gcggccgcag
    53521 ggagacacgc gtattgagat tcagctgccg gcagccagtt tggatacgca gaagaagcgg
    53581 gctgcttatg aagctgcact gacagctctc gaggaagaga atatcaatct gctgaagatt
    53641 cgtcaggcac ttgccctgcc ctctgaacag cgtcagcagg catttgagca gtatgccaaa
    53701 ggcaatgacg tccgcatgca gattctgacg gatttggcgt ctatttatga tcagcgaaag
    53761 catacccagc agcagcggga tcaactaatt ggagagatgg acaatgtcaa gacaaaattc
    53821 agtgctgccg gacttgacta tgagacgctc cagtatgacc taagggtatg gagcaaggaa
    53881 gaggcggcaa aacagaaaga aagcatcgag acctatgtaa agaacaatat taaagatgag
    53941 acagcggcag agggtactct gggacagcgg cagaaagaag ccgtttcgct gatcaatgaa
    54001 tatctcgaac tgtatagccg atgggttccc gttgcggatg ctttaaccag gccggaaaca
    54061 ggattgaatg tacaatggga caatgctgtt aaaaaactgg cgcagctgaa tctgagtatt
    54121 gagttgtttc aggatgtgct tgaaatgccc aaagattcac gggtacggcg ggaaaagctg
    54181 gaggagatga aaaaggccta tcccgaaaga agcagcaaga tcgaagcggc tgtcgcggca
    54241 tttgatgcct atcgtgtagt cggcgggcgg ctggacgatc cggaagacct gaaacggatg
    54301 ctcaagggct cgggagtatt ggaattccgc attctgccga caagcgaaga aggcaattcc
    54361 gcggaatttc aggcctatct tgaggcactg accaccaaag gccccaagca ggcatctgat
    54421 aaccgttata tctggtgtgc tatcgaagat cctcaaactt ggccggcagg attaggcatc
    54481 accggcacat ttggagagaa aaaatatgtt ctttgcagca atctgcctaa cgaaaagatg
    54541 ctgcatgaag gcgaaaaaaa atggaaactt aagaaggcat acccgacctt tgatgaacag
    54601 ggccgtcgag ctatcggatt tacatttgac gaaatagcgg ctaaccgttt ttatgctgtt
    54661 accagcagca ataacgacag gcctttggct attcttttgg atgatcaggt catatccgct
    54721 ccgcgtatta acagtgccat ccgctcttcg ggcattatta ccggttcatt ttcgcagacc
    54781 gaagtatcgg atatggtcaa taagctgaat gccggctcct tccctgcacg gctcagcgaa
    54841 gtccccatct cagagaaaag catcagtgca acaattggga cggataaccg cgacaggggt
    54901 attcgggccg gaatgattgg tctggcagct gttgccgtct ttatgctggg ctattatttg
    54961 ctcagcgggg ctattgccga tgctgctttg gtgctgaaca ttctgctggt gctgggcgtt
    55021 atggcgatgc tgcagtcgac ctttacaatg cccggcatcg caggcctgct gctgactatc
    55081 ggtatgagtg ttgacgcaaa tgtgctgatt ttcgaacgga tacgggaaga gcagcagcgg
    55141 ggttcttcgg tgaaggcggc tatcgcaaac ggttattccc ggacatttcg aacgattttc
    55201 gatgccaatg taacaacgtt cggcgttgct gccatactct atatggctgc gtctgaagaa
    55261 atcaaaggtt ttgccatcgt actgatgctg ggtatcttag ccagtatgtt taccgccctg
    55321 tttgtaacgc gagtgatttt cgactggctg accagccggc ggatattgac ccgcccgcta
    55381 atgatgtttc gaatgttcca gaatgccaat attaactgga tgagactgcg tccggtattt
    55441 tttgtcattt ccggtattct ggttataagc ggtctgctgg tcttcttttt aagaaatgac
    55501 agcacaaaca gcaaatatga tatcgaattt atcggcggaa caagtgttga aattgaatta
    55561 aaggaaggca ccggcttcaa tcgggaaaag gtagagcaga tattcaaggc ctatgccaat
    55621 tccatcggca acagcgcctt gactgccgcc aaaatttatg aagtcggcaa atccggccgc
    55681 cagtttgaaa tcagcactac cgagaccaat aaaacaaccg caaccattgt gctgaacagc
    55741 gctggtacgg aaacagcggc agctctggcc gaaaagattc gaaaggcatc cgccgaaaca
    55801 ggcaaactgc tgtcgaatct taacgttgtt tctatcggcc cgaatacctt caaggtttcg
    55861 accagccgct taaatgctgc cttggtccaa gaggtattgt ctgcagctgt cggtgcagat
    55921 ggaactgtca ccaatgtcga ggtccaggaa gttgtcagtg atgcggttcg cgaagcgttc
    55981 aaagattatc tgacagttcg cgaaaatctc ggattgtcgt ttatagatga gcagaaaata
    56041 gaaacaagtc ttgatgatgc cgctctattg tcggattact tgggcgggat taaactgacc
    56101 tgccagcttc agatggaagc cacagcagcc gaactggaag ctcgcatcag cacgctgctg
    56161 tcaaaaccgg acatgcagga attggtacgg tatccgcaca aactgctgaa aacagatttg
    56221 accgaaccgg ccgctgatga acctctcaaa agttttgtct atgtcagcgt tcatcccgaa
    56281 gccggctatc gcgagctgga ccagagtgag tggcagaatt ttgtaaacaa cgaaaagacc
    56341 aaattggccc aagccgcttc gctggaaacg tcgctttcac gcgttacgca aattgatcct
    56401 tcgatcggct cgcaagccaa acagcgcgcg attgtggcca ttcttctttc actgctggcg
    56461 attgtgggat atatctgggt gcgcttcggc acagcccggt acggcatggc ggcgattatt
    56521 gcccttgttc atgatgttat tattactctt gggctggtga cggcatgcac ctatatcgca
    56581 cccactgctt tcggcaaagc ccttctgatt ggcgacttca aaatcaatct ggagatgatt
    56641 gcggcatttc tgacgattat aggatattca ttaaacgata cgattgtcgt tttcgaccgc
    56701 atacgcgaga accgcggaaa actgaatatt ctgacgccca gcctgatttc ctcgagcatt
    56761 aaccagacgt tgtcgcgaac aattttgacc tcctttacga catttctggt ggtgctggtt
    56821 atgtacatct ggggcggtga ggggcttcgc ggctttacct tcgcaatgat ggctggactg
    56881 attgtcggta cctattcttc aattgccatt gcatctccga ttttgctaat gggaaaatct
    56941 caagagcagt agaaaggagt tcccgcaaaa caggaatttc aaggccggaa ccacagggtt
    57001 tcggcctttt taattgcgcg gcgttttccg ccctaaccaa gaagaaacaa ttatagcagc
    57061 gactgtatcg ccggatgcgt tgaccatagt tgccggagga tcgaccaatg tgcccacgat
    57121 tgaaataata ggcagtgctt ccgcgccaaa accatagaag gaaacaatca gcacttcacc
    57181 cagaaaacct ccgccgggga taccgctcat aactacgccg cacaaaagcg aaacacctat
    57241 ggccgtaaga taggtttcaa ttcctgcaaa ggggtggcca tagatcgaga acagaaccgc
    57301 tattttgaca acagccgcca gacaggtgcc atccatatga ataatggctc ccagcggcag
    57361 aataatttct cggatttcct cgggtattcc aatgcgtcgt gcagcttcca tattgaccgg
    57421 cagggccgct aagctgctgc cggtacccca agcggtcaaa gcaaccggcg gtatatgggt
    57481 ccagaattgc cgcagggagc ttaccccccc tgcccataaa acatacaccg aaaaaccgag
    57541 gccaaaatat aaaaaagcca acggataata cagcatcatc gcacgaatat agatatccgc
    57601 cagctgcggt tttaatgctc cgaccagata agcaaaatat gcgccaagtc ctatgggtgc
    57661 atacagcata atcagagaaa tcaccttgcc cataaccgct gcaccggaac agaggaagtc
    57721 ctggaaaact cgtccctttt ggccggctgt ctgcgatgcc aagcctacaa agagagaaaa
    57781 gacgatgagt gccagcatgt tttgacggct caagagggct gtaaaatcgg gtactgtaaa
    57841 tgtttcaacg aatttttctg cccaatcggc cgagctggtt gtatttgttt gcgtcgtctg
    57901 cagtgttatg ccctgccccg gatcatacag tttgacagcc gtaagcatca ggcaggagga
    57961 gatgatgccg gtcaatgcaa aaaccagcag catcgccccg gcaatttgcc ctaagcggcg
    58021 gatatttgca ctggaagcca cggccgaagc aatagagaaa aaaaccagcg gaacagccgc
    58081 acagaataac agatttagga aaaggtcgcc aaaaggtttc agcagacggg ctttcgaacc
    58141 gaacacaaca ccgatgactg agccgataag cacggctcct gtcaggacaa tcgtaaaccg
    58201 ccattgctgc aggaaagtct ttggcttcat aatttgggca ttcaaagctg cttattacgc
    58261 ccggcgggat tcgaacccac gaccccaggt ttaggaaacc tgtgctctat ccaactgagc
    58321 tacgagcgca caactgcaat tttagtctgg ccaagcgtga acttcaactg ttgtttcaaa
    58381 aaagcaaact accatattgg tgaatgggtt tgacgaatga cggccgcagc aacgccttta
    58441 atcaacgcat cttcccctgc ctgtgccgga attatcggga tttcccgcag ggcattgtcg
    58501 tagaccgatg tctgtatctg ctgttggatt ttctgagcca aaaaaggcaa aaatggcaat
    58561 acaacatatc cggccaagat gataatatct ggatcgtagc agttaatcag aatggcggct
    58621 gcccagctta tatatcggct gacatactct cgtagctgaa gagcataacg gtcgccgcgt
    58681 tctatatgta caggccaatc ggccagcagt tcttccggag tgtgggaaat agaattggca
    58741 aatgcagttt ttgttccatg ttgaatatct tgcttgattt tagcagccaa agccggcccg
    58801 gagataatgg cttcaagaca tccttgatga ccgcagccgc ataacggacc cgcaggatct
    58861 gcaataatgt ggccgatttc accgtatctg cctgaggccc caaaaaccaa ttgcccgttc
    58921 atatagagtg tcgatccaac accattagcg gcattgatgt ataaaatgtt gcgagaggcc
    58981 agttcgggct ggacttcaaa ttctgccaga agacgaacac ggttgctgaa gacagagaca
    59041 ggaaatggca aaacggctga cagtttttct ttcaaattta tatttttcca tcccattggg
    59101 ctggacagcg agatgttgcc gtcgggagtt accgaaccgc ttaaagtaac acctgcaccc
    59161 ataatctgtg atccgtcaat acgatggtca tccagcaaat cgggcaatcg gtctttcagc
    59221 agagtcagaa catgcccaat tgaatgatcc tctcctaagg gaattcgaat cttgtttttg
    59281 agaaatccaa caaaattaaa taatcccaag actaaatagc ttggattgat ttcaatgcct
    59341 atcaaataaa gataatccgg atttaattcc accaatattg gttttggtcc ccttttgctg
    59401 ctgctgccgc ggcgctcggt aacaaaacct ttttcacgca gacgggctac gatagtgctg
    59461 gcagttgagc ttctaatgcc ggcaagccgg cagagttcag actgcgacaa tcttccatgc
    59521 ttgcgaagca tagatagaat gagatgctcg tttctctggc cggcactctt agaatcaatc
    59581 ggactattaa tcataaatca ctctttctga ccaaaattat ctcacaacat gtgctatttt
    59641 caaatccttt tcgaatgaag tttgaaaaat atgcttgaaa agctgttgga attatgacaa
    59701 taataatctc atggcgtgtg atatttattg ctgcctaacc ataatgttat taagttcttt
    59761 ttttatatat agatacgtca attgtcgcag taaataaagg gaaaataaca tgacgaactt
    59821 gttcagcgtt aaagataagt gtatcgttat tactggggga gccggaatat tgtgcggagc
    59881 cttggcggag gacttggctc gaagcggagc caaagtgtgc attgccgatt acgatgaatt
    59941 ccgtgccaat caactggcag aaaaaattaa tgccgctggg ggatttgcat tagccgttcg
    60001 gattaatgta ctggaaaaaa aagaagttga ggaagcattt gcctgtgctc ttgaaagttt
    60061 aggccgcatc gatgccctta tcaatggagc cggcggcaat aaaaaagagg cgacaacttc
    60121 cgaccagctt gcgttttttg atattccggc agatgctttt cgctgggttt ttgatttgaa
    60181 ttgcttgggt acaatcttgc cctgtcaggt tttcggccgg tatatggcaa aacagaaaga
    60241 aggcgtaatt ctgaatatct ccagtatgaa tgctttcagg ccattgacca agatctcggc
    60301 ctactccgcc gccaaagctg ccgtcagcaa tttcacgcag tggctggcgg tccatatgtg
    60361 tcaaaattat tccacaagca ttcgggttaa tgccattgct ccgggattct ttttgaccga
    60421 acagaatcga ttcttattga cggatgaaaa aaccggagaa ttaacccctc gcggccgaac
    60481 cattatcggc cataccccga tggggcgttt cggacagccc gaagaattat gcggtacggt
    60541 gcgatggctg ttgagcgatg catccaaatt cgttacagga accgtaatac cggtagacgg
    60601 cggcttcagc gcattcagcg gtgtttgata catttcaaaa aaggaagact gatggcaaat
    60661 cctgctcaag ctctgattga tatgaaaccg accaaacctt ttctggtcgc cattgattct
    60721 gacggctgtg cattcgacac catgggcatc aagcatcggg agtgcttctg tccgatgctg
    60781 attgcctatt tcggccttca gccggttgcc caggcggcac gggagtgcaa ggactttgca
    60841 gatttattca gcaaaacccg cggcgctaac cgtcataaaa caaccattcg gattctgcag
    60901 gagctgcttc ctacccatcc tatggtaaag gagcgtggat ttgctgtacc tcaatttcct
    60961 cattatgcag catgggtcaa taatcccaaa agtcttttga gtaatgaagg gttaaaaaaa
    61021 gcaatcgatg aaacgcaaaa tgaagaggcc aaaaaggagc tccagacggc gctgacttgg
    61081 agcaagcgtg tcaatgagct ggtggctgag attgtcaaaa acatcccgcc ttttccgtat
    61141 gtgcgggagt gtcttgaaaa gatttatcct gctgccgata ttattgtctg ttcggccacc
    61201 ccgacggaag ccctgcatcg ggaatgggcc gaacacaatc ttgcccgata tgcgaaagtt
    61261 atcgccggcc aagagatggg ttctaaggcc gagcatcttt cgataatgcg gcagaaatat
    61321 gcccctgata aaattctgat ggtgggagat gcgctgggcg accagcaggc tgccgagaaa
    61381 aacggcgttt tgttttatgc gatcaatccg ggacatgaac cggccagctg gaagcgattt
    61441 tatgatgagg cctttgataa atttgtcttg gggacatacg ccggagacta tgaaagacgg
    61501 attaagatag agtttgacgg ctatctgcct caattgcccc cttggctgtc aaaaggataa
    61561 actatgttat attttgctcg cggaaatgaa aatgccttct tgtctcacca tgaccttcaa
    61621 gaaggcctta ttacggcact ggaagcattg ggacctcgcc gcaaggtgct ggcaatcccg
    61681 cctgatataa cccgtttttt ttcgcgggct ggagagctga cccgttatgc ggcagactac
    61741 tacggcaaaa aactgacgga tatcctgccc gctttgggca cacatgctcc catgacagaa
    61801 gaggaaatcc ggcggatgta tgcggatgta ccgctggagc tgtttcgcca gcataactgg
    61861 cggaaggatc taaaaactct cgggcgcgtc cctgccgagt acatacggga aatcagcgaa
    61921 ggccgggtag attatgactg gccggctcag gttaatcagc tgctgacgga tggcggtttt
    61981 gatttgattc tgtcaatagg ccaagttgtc ccgcatgagg tcatcggaat ggccaactac
    62041 aataaaaata tttttgtggg taccggcggg gccgaaggca tcaataaaag ccatttcctt
    62101 ggtgctgctt acgggatgga gcgcattatg gggcgtgccg atacccctgt gcggcgtgtt
    62161 ttaaattacg catcggaaca ctttgctgga aatctgccta tcgtatatgt cctgacagta
    62221 gtcagtgcag tcgataacag gcaggcagtg cgagggctgt ttatcggaga cgacatcgaa
    62281 tgctttaatc ttgccgccgc cttgtcatta aaggtcaatt tcaaaatgct tgacaaaccc
    62341 ctgaaaaagg ccgtggtgta tctggatccg cacgagttta agagcacttg gctgggcaac
    62401 aagagcattt accgcacacg gatggcaatg gcagatgggg gcaagttgat tgttctggcc
    62461 ccgggattga aggaatttgg agaagataaa gaaattgacc ggctgattcg gcaatacggt
    62521 tacgtcggca cagaccgggt gctgcagctg gtggaagcca atgcagacct gcagaataac
    62581 cttagtgccg ccgctcattt gattcacggc tcgtctgaag gccgatttga cgttatctat
    62641 tgtccgggac atattacaaa ggaagaaatc gaatcggcgg gcttttctta cgccgaactt
    62701 gacgctatga tgcacaaata caacccctgc ttgctcaaag acggcttcaa tgaagtggat
    62761 ggagaggaaa tattctatat atccaatccc gggctgggtc tgtgggcata cagggaacga
    62821 tttaaataaa agaatccaaa cattttatcg aggagaatca cagatggcca aggcagatat
    62881 agcagtggta ggattagcag tgatgggcga aaaccttatt ttgaatatgg aaagcaaagg
    62941 gtttaccgtt gcctgctata atcggactgt ttccaaagtg gatgatttta tcaatggccg
    63001 agccaaaggc aagaatatta tcggctgccg gagtattgaa gaaatgacgg ctgccctcaa
    63061 acgtccgcgc aaagtaatga tcatggtcaa ggcaggcaag ccggtggatg attttattga
    63121 gcagcttttg ccacatttgg gagatggtga cattatcatt gacggcggca acagccattt
    63181 tcccgacacc atccgacgta cccaatatgt tgaaagcaaa gggaaacttt acatcggcac
    63241 aggcgtttca ggcggcgagg aaggcgcttt gcgggggcct tccattatgc cgggcggctc
    63301 tcctgcggca tggccgcatg ttaagcccat ctttcaaaaa attgccgcca agaccgacaa
    63361 gggtgaaccc tgctgtgagt gggttggaga aaacggcgca gggcattttg tcaaaatggt
    63421 gcataacggc atagaatacg gcgatatgca gatgatatgc gaaacttacc agatgatgaa
    63481 gcagggactg gggatgagta atgctcagat gcatagtgtt tttgccgaat ggaacaaagg
    63541 ggagctcgat tcctacctga tagaaattac gcgcgatatt cttgggtata aggattccga
    63601 tggcaatgaa gtggtcgatt tgattttgga tgcggcaggc cagaaaggca caggcaaatg
    63661 gacggttctg gcagcccttg acagcggcca gccgctgacg ctgattgcag aggcggtatt
    63721 tgcccgctgt ttgtcagctc ttaaagaaga gcgtgttgcc gcttcaaagg tgctggccgg
    63781 tccgaacaaa cagttcaacg gcaataaaaa tgattttgtc aacgatcttc gtcaagcttt
    63841 atatgcctca aaaattgtca gttatgcaca gggctatcag ctgatgcggg cagcggctaa
    63901 ggaatacaac tggaatctca attacggcgg gatcgccctg atgtggcgag gcggatgcat
    63961 tattcggtct gctttcttgg gcaaaatcaa agaggccttt gataagaatc cgaatttaag
    64021 caatcttctg ctggatccgt tcttcaagga agctgttgtc aaggcacaag atgcctggcg
    64081 gcgtgtggta accagcgcag taaccattgg aatcccgatg cctgccatca gttctgcatt
    64141 ggcctattat gacggctacc ggagtgagcg gctgccggcc aatctgcttc aggcccagcg
    64201 ggattatttc ggtgctcata cctatgaacg ggttgataaa ccgagaggtc aattctttca
    64261 tacaaactgg acaggccgcg gcggtgatac ggcggcatct acctatgtgg tataaattag
    64321 aaaggatttt ctctcgcagg aggcaaagcc gatgaagaca ccgtatatgc acgaagattt
    64381 tcttctgcag aatgacacag ctaaagagct gtatcacaat catgcagaaa aaatgccgat
    64441 ttatgattat cactgccacc tgccgccggc ccagatagcc gaagacaagc ggtttgccaa
    64501 cctgactcaa atatggcttt atggagacca ttataaatgg agagcgatgc ggaccaacgg
    64561 catcgacgag agatactgca ccggcgatgc ggacgaccgc cagaaattcc ggcactgggc
    64621 tcaaacagtg cctttctgca tccgcaatcc gctgtatcac tggacgcatc tggagctgaa
    64681 aaaacctttc ggaattacgg acatgctgct cagtgcagag accgcagaca ctatttatga
    64741 cagatgcagc gaaatgctcc agtcacccga attcagcgtc cgtaatatta tgcgcaaatg
    64801 gaatgtccgt ctggtctgca caaccgaaga cccgctggat atgcttgagc atcataaggc
    64861 gatccgtaaa gacggctttg aaattgccgt tcacacggca tggagaccgg acaaagccat
    64921 gatgccggaa aatcttgatg ttcagaatgc ttggattgac aagttggctg aactaactaa
    64981 catagaaatc aacacatttg accgatatct tgaagccatt cagcagcggc ataactattt
    65041 tcacgaacac ggctgccgtc taagcgacca cggtctggat cgtccttttg cggcggatta
    65101 taccgacaag aaaattcagg caatctttct caaaattcgt tcacggagtc ccctgtcacc
    65161 gcttgaaatt gaccaattca aatcggcaat gatgatcgaa tttgggaaaa tggattatcg
    65221 caggggctgg gtgcagcagc tccacatcgg agcccttcgc aacaacaatt cacggatgtt
    65281 cgccaaactc gggccggata cgggatttga ttccatagcc gattatgaaa ttgccctgcc
    65341 gctatcgaaa tatctagaca aacttgccag tcaagatatg ctgacaaaaa caatcctgta
    65401 caacctgaat ccaaaggata atgaagtgat tgccactatg ctgggcaact ttcaggggga
    65461 tggtatcccc ggaaagattc aattcggatc ggggtggtgg ttcctcgatc agatggatgg
    65521 gatgacaaaa cagatgaatg ccctctcgaa tctggggctg ttgagccgat ttataggcat
    65581 gctcactgat tcccgcagct ttttatctta ttctcgccac gaatattttc gtcgaatatt
    65641 gtgcaatctt cttggtgaag aggttgaaaa aggattctgg ccaaaggata tgacactgct
    65701 gggacggatg gtggaagata tctgctacaa caatgctgta aattacttta gcatgaaact
    65761 ctgattgttc ttctgtttgc ggcttgacga tattctgatt tgctgataca atacgtcaaa
    65821 aacaggcgta aggaaaggat ctatccatga cacagaatct gttagccata ttcggtctgc
    65881 cgggcggcgg agaatggatt attattttga ttatcgcggt tttgattttc ggacgaaggc
    65941 tgcctgaaat cgcccgcgga cttggtaaaa gtattaccga atttaagaaa ggcataaaag
    66001 ataccgagga tgaagtcaag gacgctctgg aagaatcgga taaaatcgcc gaaaaacata
    66061 cggaaaagaa aatcaatccc ccttccgatt aattgaaaca gacctcttta ttttacctgc
    66121 cttttacgcc tccatatccg gcagacaatt atactgcttt cgtagagaaa atagagcgga
    66181 accgccagag aaatctggga aacaacatcc ggcggtgttg cgattgcgcc gacgacaaaa
    66241 caccctaaaa aaacatattt ccgtacacgg cagagtgttt caactgacac cagccccatc
    66301 cgttctgcaa aaacaattgc aatgggtgtt tggaaagctg cgccgaaaac cagagacagt
    66361 atcaatataa aattcacgga ctttgtcagg gaaggctggt aattcagata atcgatgccg
    66421 ggattgaaac gtacaaagaa cttaaagaca aaaggcgcga ttacagttaa aaaaaagagt
    66481 acacccgtcg taaaaagaaa tgcgctggca ggagcgacca ttttgacaaa acggcgttca
    66541 tgagtataga gccccgccga aataaatgcc cagatttgat aaaacagcca cggtgaacag
    66601 ataagaatcc ctaataccag agatgccttc aagtaaacca taaacggttc cggcacatca
    66661 atagcaagca atttcatttc aatgttcaat tctgtcatcg cccttttata cggccataaa
    66721 ataaactgca gaaaccactt gccgaaaacc agaccgatta tagatgcggc ggctaatccg
    66781 accaagctga gaatcagacg tgctcggagc tcctccagat ggtctcccag actcatactt
    66841 ttgagttctt cttcatcgtg gtgtcttcgt ttgctttcgc tcatatttat caaatcagcc
    66901 gcagattttc ctgtacttat tatagaatat agagcagata gtttaaggaa aacaagagct
    66961 attggcaact atttttaata gccgccggcg tagactttct tataatgaag ttatgcaata
    67021 taaaacaatt gtaatgttga ttgctggcat cttgttttgt ttgggcagtg caggatatgt
    67081 cgtgtttaaa agtatcctac ggagcaaaga caggaatcgt attggggaat acaatgagat
    67141 gtttgaagtt gacaatccgt atttcctgaa atatcaacgt tggatccgta tttgctttac
    67201 tgtggttatt atcagcatgc tgttgctttt tgtttgtatt gctatttaat ggttcaatga
    67261 tagcgttgta ttttttttct ttacaaatct aaatatctgg tatacaaaaa agtaatagtg
    67321 gagatataag tgaatgtttt acacaaaata ggagagaatt gttatggagc aaaatgcaga
    67381 aaatcaacct acttcatctg aaaatcagcc gcagccatct tctcaatcgc ctcaatcttc
    67441 caatatcagt caagtccctc aggattccag aaatatggct atgctatgcc atttactggg
    67501 actttttaca agttttgtcg gtccgctgat tttgtggctg ataaaaaagg atgaggataa
    67561 gtttgtggac agccaaggta aagaagctct caacttccaa atcacggtaa taattgcagg
    67621 tattgccgcc ggcttgctgt cgtttatctg catcggaata tttgtagcga ttgccgtatc
    67681 tatttgcgat atcgttttct gtattttggc ctgcgtggct gccagcaaag ggcaggatta
    67741 ccgatatccc ctaagtctgc ggctgattaa gtagaaactt gctcgtttcg ctcaaaaagg
    67801 cattttattc ccttcaaaaa cgggataaga tgcctatttt gtatctatag gaggataaaa
    67861 tgccggcttg catatacggt gttttactgc tattatgatg ttatatggga aaacaatcca
    67921 aacaaaatcc ggctccttcc gttcagaata aaaaggcctg gcacaatttt gaacttgtag
    67981 aaaaagttga ggcaggtatc gttctgcttg gaacggaggt aaaaagtctg agggagcggc
    68041 aatgcgactt ggacggcagt tatgctcgtt tagcagacgg agaggtgtgg ctgctgggct
    68101 gtaaaattgc cccgtatgaa cgagggggct acacaaacca tgagcctctt cggaagcgca
    68161 agctcctgct gcataaaaat caaattcgaa aaatccgcac aaaactggag cagcggggat
    68221 ttacgcttgt tccaacccgg atttatttta acgatcgcgg aaaagctaaa gtggaactgg
    68281 cgttggcaac cggcaaaaaa ctgcatgaca agcgtcaggc ccttcgggaa aagcaggcac
    68341 gcaaagacat cggcagggat atgaaatata aataacgtat ttctgtaatg gagcaaacca
    68401 tggcggacaa tggcaagaaa atcatcatcg atgaagattg gaaagcccaa gcacagcggg
    68461 agaaggaaat tttaaaagaa caggaaaaac gggaaaagga gcaagccagt tatccgggtc
    68521 ttccgtcggc agattttgcc ggtctggtaa gtatgctggc tacacaggca ttttacgctc
    68581 tgggtgttat aggtccgcag gacggcagag aagaagaccg tcagccggac tgggctatgg
    68641 ccaagttcaa cattgatatg cttggcgtgc ttgaggaaaa atgcaaaaat aatctgtctg
    68701 acgaggaaga caagctgctg aagggaacat tagggcagct gcgcatgctt tttgttcaac
    68761 tatcggaaaa atgacctgtg gcttccattt tggataaaat cctttctgat aaacgggcag
    68821 aagtccagct gcgccgtcag cagcagccaa tagaactgct gatggaaaaa gcgatgggac
    68881 ttaaaaaatg ccgcaatttt tatcaggcag ttacaaaacc taatccgcga ggactgaatg
    68941 ttattgccga ggttaaaaag gcctctccgt cggccggcct gatacgggca gattttgatc
    69001 ccgttcaaat agcccggatt tatcagcgat gcggtgctga tgccatcagc gttctgacag
    69061 atgaaccgta ttttcagggc aagctggaat atctcagtca aatcaaggaa gctgtctgtc
    69121 tgccagtgct tcgcaaggat tttattatcg atccctatca agtttacgaa tcgcgggcag
    69181 caggagcaga tgccattttg ctgattgcag aggcactgac cccttcgatg ctgatggatc
    69241 tgctgatact ggctaatgag ctgacgctga cggtgctgat tgaggtacat aatgctgata
    69301 cgcttctgca gatgcgaagc atcatcggct ttccccagaa aaattacagc gtgcttggga
    69361 ttaacaaccg ggatcttgcc acgatgaaag tggatttgaa taccactggc cggctggccg
    69421 aactggtcga taaccgccgc gagttgatcg ctgaaagcgg gatacaaaca agggcagatg
    69481 tcgagaagct catacgaatc ggcatcgggg gcgttttaat cggtcagacg ctttgtcaga
    69541 gcccagatat tccgtctaag tttaacgagc tgttcggcag ctgcgaaggg taaatgtgtc
    69601 gattttaatc ttctttgcga ttggattagc cgctggcgta ctgggcggcc tgctcggaat
    69661 cggcggatcg attgtcatta tccctgcctt aaccattcta tatggcgaaa accagcatct
    69721 ttatcaggca tcggcaatga tctgcaattt cttcgtctct gcagcagcta ttattgcaca
    69781 ctggaaagcc gagtcctttg acaagaccgt attgaaatgg atggtgccct tggccgttct
    69841 gggcatttgg ggaggagttg cgttgagcaa ctgcacaatt tttgctgcag aaaacagcta
    69901 tcttctggca cgtgtattcg gagcgttcct gatttatgtt gcggtctata atatccgtca
    69961 tctgtacctg cactggcgag gcaaaaacat tacactccat ggtcctccgg ttgcgtcgat
    70021 ggcgcatttg gttttgtcgg gattctgcgg tgcggcaaca ggtctggctg caggtctgct
    70081 gggcattgga gccggaacca tagcgacgcc cctacagcag cttttactgc ggatgcctct
    70141 gtgcaaagcg atgagcaact cggccggtat aattgtcgcg attgcatggc tgggagcaat
    70201 ttataagaat cttaccttag cccagcatgg tctgcattgg agcagttcgc tgcatatcgc
    70261 aatttttgta attcccggtt ctatcgcggg tggatatatt ggaggtcatt taatgcatct
    70321 tctgccgaga aatcttgttc gtttcgtctt tattcttgtg tgtgcgctgg ccgccgccaa
    70381 actgctgaca gtctgccctg cttgatatgg aacaggcggt tttctgcaga cagagttatt
    70441 ctatcactcg aaccgaaagg atttctaccg gttcgactgg cacatcatta tagaatcctt
    70501 ttcgggttgt ttttacagcg gctatcttat ctaccacatc aagaccttct acgactctgc
    70561 cgaaaaccgc atatcctatt ttatctggct cggccccctg acaatcgaga aaatgtt
//
LOCUS       DAOTBO010000003        61833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_383_length_61833_cov_5.731264, whole genome shotgun sequence.
ACCESSION   DAOTBO010000003 DAOTBO010000000
VERSION     DAOTBO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 61833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61833
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_383_length_61833_cov_5.731264"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..157)
                     /locus_tag="PLE88_00675"
     tRNA            complement(82..157)
                     /locus_tag="PLE88_00675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(122..124),aa:Asn,seq:gtt)
     gene            380..1255
                     /locus_tag="PLE88_00680"
     CDS             380..1255
                     /locus_tag="PLE88_00680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOM75013.1"
                     /translation="MVKSTIVDIDGYTILRRLGTGARSTIYLAVQEDSKTTVAIKRAI
                     LESPEDTRIFEQMETEYKVSRQIDHPYIRKCYDIIRNRKLLRTNELILIMEYFEGQNL
                     EDQQRLSMGDVLLVFRMVAIALNAMHEKGYVHCDIKPNNILFSPKGGIKIIDLGQSCR
                     LGEIKKRIQGTPDYIAPEQVRREHLSHRTDIFNLGATMYWALTGRNVPTLIPKKNEMG
                     IAIDETASFKSPNELYRKIPKTLSDLVMECVQEKPADRPSNMSEIIDRLDVMIREIFG
                     SRTSGNAPKVNTTLS"
     gene            1194..2072
                     /locus_tag="PLE88_00685"
     CDS             1194..2072
                     /locus_tag="PLE88_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM75014.1"
                     /translation="MKSLGPEHPAMPQKSIQLCREGAELNRSCRFRSGNVIHLPDIGK
                     VIVAGDLHGHRRNFERIQKFADLGNNPWTHVVLQEIIHGGPEDDYGGCLSWQLLFDAI
                     RYKIRFPDQVHILLGNHDTAVISNSSVLKSGKEMNRAMQAAMRRQFGQHYDSVYAAMI
                     EYLLSQPLVVRCPNRIWISHSLPADDYLETFDLSIFNRPYTMEDIERPNPVYLLTWGR
                     RHSENTLLRLGQMLDADIFILGHQPQETGWSMGGSNTLIIASEHAYGCILEFDLSKTY
                     TASDLASHIIPLGTIE"
     gene            complement(2096..3910)
                     /gene="lepA"
                     /locus_tag="PLE88_00690"
     CDS             complement(2096..3910)
                     /gene="lepA"
                     /locus_tag="PLE88_00690"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOM75015.1"
                     /translation="MQNNPIPIQQIRNFSIVAHIDHGKSTLADRLLERTGAISEREMK
                     EQLLDDMDLERERGITIKASAVTMQYTYQGTVYMLNLIDTPGHVDFHYEVSRALTACE
                     GALLVVDASQGVEAQTVANAYLAVESGCEIVGVINKIDLPAAQPDRVAEEVEHVLGLN
                     RSECIFISAKTGQNIDALLEAVVKRLPPPQDKSDCPTAALIFDSHADEYRGVICYVRV
                     FEGSLKTRQRIRLMGSGRSYTIVELGKFTPRMTPVEQLGCGEVGYVIANIKNLSDVTI
                     GDTITDAAEPAAQPLPGYKPPMQMVFSDFYPGGSTDYPRLRAAFEKLALNDASFSFVP
                     QNSPALGFGFRCGFLGLLHMEIVQERLERESDIDVVQTAPTVSYEILMTNGQLKRIDS
                     PSELPDRQKIAEIREPMVKLEIITTRESIGGIMKLAEERRCVLLKTEYISPTRVMMEY
                     KAPLAEIVYDFYDVLKGISRGYATMDYEFLGFEAGNLVRIDILVNGTPVDALSLIVHR
                     TKAEQRGRKLILKLKKAIPRHQFEIPLQVAIGGKIIARETIKAFRKDVTAKLYGGDVT
                     RKMKLLKKQKEGKKRMKSIGMVNIPQEAFMSILDTSEE"
     gene            complement(3942..5786)
                     /locus_tag="PLE88_00695"
     CDS             complement(3942..5786)
                     /locus_tag="PLE88_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOM75016.1"
                     /translation="MRSVFLPTTPQEISALGWDRPDIILVTADAYVDHPSFGIALIGR
                     WLEYHGFKVAVLDQPDWRSVEPFRTLGRPRLFWGISSGCIDSRLNDYASLGNKRRQDV
                     YSPAGKTGRRPERPLLAYAARAREAYKGVPIILGGLEASLRRLVHYDYIEDKLKRSVL
                     TDAKGDLLVYGMAERTILEVARRLDSGMSINEMTDIAGLAYRVVGSAPIPPDAVCLPS
                     LQELEKNNQLFMNFQLAYQQEAIQTGRPVIQDQTPGLIVVNPPADPMSQQELDVLYSL
                     PFARCWHPKYDKQGGIPALKPIQFSITTHRGCFGGCSFCAIYFHQGKQIASRSEASIL
                     DELDKLIRHPQFHGTIQDIGGPTANMYGMACCKAAKCKRMSCLYPQPCPKLPNSHRAV
                     VSLLKKILAWQKAQKKKVHIFVASGIRHDLALHSSEYLELLAAHFIGGHLKVAPEHIS
                     QSVLDMMQKPHLETFEIFEKAFVQASRKAGKEQFLVPYFISSHPGCSLEEALQLTEYL
                     VRRGWRPRQVQDFVPVPLALSTAMYVSGLSPAGKAIHIPRRRAEKRLQIALLQYYDKR
                     HFKIIEAFLSPRGKHKLLAQIRRLQIAQDTSGHRQKTGGKKGLIEDGT"
     gene            5965..6036
                     /locus_tag="PLE88_00700"
     tRNA            5965..6036
                     /locus_tag="PLE88_00700"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5997..5999,aa:Ile,seq:cat)
     gene            6089..7924
                     /locus_tag="PLE88_00705"
     CDS             6089..7924
                     /locus_tag="PLE88_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOM75017.1"
                     /translation="MVVPVQKVTWTEGCFIWPVCPLLASPYSADTTPLAQLLGDLQRH
                     LHIRGYLVRNAFGPAAMRIQRDSSIRHPEAYRMVIEPDAITVISASDAGAYYGVQTLR
                     DIVYLCGRQIPCCVIDDQPVFSRRGVYHDCSRGKVPTLHTLKQLVERLAHWKINELQL
                     YVENVFTFVRHPLIGKGCSPFTPEEILALQDWCRQHHIRLVGSLASFGHLEKILCLPP
                     YRHLGEMYGLDNSSGSTTLCPIDPGSIKLLAELYEEYVPLFEAEDFNVCCDETTELGK
                     GRSRRRADKIGAGQVYLDFVLNLYKLCQRYGKRMNMWADILLEHPELLDKLPHDIVLL
                     NWEYEADGTNIRQTPKIAQKGLPLMVCPGTSSWLTHGSRLDNAMRNIAGFAQQGRRCH
                     AEGLLNADWGDRGHRNLLGISLHGFAYGAACAWNPADADNSRFTEHFCRLTFGQCESH
                     LAKAVRILGTTYRTCGAPRRNGSFLFEGMTEPMPASEAPSSSAIDKMTSAGLKKIVSQ
                     LSSDRLWPTAAASLPEFERLALAELKLAAKMDLWSAQRALAAKNLRTGQPVSAKQIRL
                     LAEQMKQIEAEFSSLWMARCKPSRLCDNLRLFKKTRQQMLRLIPH"
     gene            7937..9121
                     /locus_tag="PLE88_00710"
     CDS             7937..9121
                     /locus_tag="PLE88_00710"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOM75018.1"
                     /translation="MNKPKSIADLAKRKHLRAAGLMSGTSADGIDAAIIDIHGKNISV
                     AGFGTVPYSPAVRTAILDLCNPAGSRLDNICHYNALLGELFAKALIRVCRKESIPLDS
                     IDLIGSHGQTIYHNPAGRRQAGRLVRSTLQIGEPSVIAERTGILTVADFRPRDMACGG
                     QGAPLVPFADYLLFSHRRQNRIIQNIGGIANATWLPAGGRPENTAAFDTGPGNMVIDG
                     LVRIITGGRQNYDRNGSWAARGTVHQDLLSELLTHPYFNRYPPKTCGREQFGLDYCRM
                     LVKKAARLRLNEADLLATATALTAFSIAQAYRRFLDKQPQQVILCGGGYRNRTLVKML
                     RWYLEDMELLTTNDFGIDADAKEAVCFALLGWAAVKGIPANIPSATGAHRAAVLGKIV
                     PL"
     gene            9118..10020
                     /gene="murQ"
                     /locus_tag="PLE88_00715"
     CDS             9118..10020
                     /gene="murQ"
                     /locus_tag="PLE88_00715"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOM75019.1"
                     /translation="MKDRSKLTTEQQNIRSRNIDSLTTEQIVAVINAEDKKVAPAVAR
                     ERKAIAAAADMIAARLEQGGRLFYVGAGTSGRLGVLDAAECPPTFGVRRTLVQGIIAG
                     GRRALTRSIEGAEDSAADGQTALQAKKLTASDVVVGIAACGLTPYVHGALQYANKIGA
                     GTVFITCAPEAVRHIPASIIINPVVGPEVIAGSTRMKAGTATKLVLNTLTTAAMIKLG
                     KVYGNLMVDLTAVNDKLRDRSIRIVMQLTGLSRQKAAKLLTCAGGKVKPAVVMHFHKV
                     DFQIACKLLRNHNNSLRNTIREFV"
     gene            10017..11507
                     /locus_tag="PLE88_00720"
     CDS             10017..11507
                     /locus_tag="PLE88_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75020.1"
                     /translation="MTCLHRIACGTIGWIDISITAAAIAMLFAISYVFGRDENDTRDF
                     FLGSRKIPPWAACLSFVATEVSALTIVGVPATAYSENWQYLQFFIGSAAARLAVCFLF
                     IPVFYQYQCTSIYEYLRHRFGAQTQYAGSVFFFITRLIASGVRLYAACMGVGIIMGWT
                     LEASVVLFTIVSIVFIAIGGIKAVVWAGAYQAVVFFAAGGVLLAYLLYRIEGPFAAAL
                     QMAADAGRLSIFDFSFELNRPTSFWAGSINAFFVGLAVFGTDQEMVQRLLTVENRQKS
                     QRALFMTLSAALPLLCLYLAIGTGLYIFYQQHPDIPAPAQAKEVLSHFIRLSLPAGLK
                     GLLLAAIILASIDSPLSSLAASFVSDFYRPVLAKNASERHYLTVSRTGVVLFGLILGI
                     IALACRPVENILWFAFQIFSITGGAILGVFLLGTLTKIQANYSNIAAMIITAASMTVL
                     LLLKHFEKIGLAWSWLIVVGTVQTMLLACLFSRLGAVLFTAGRAGR"
     gene            complement(11482..12972)
                     /locus_tag="PLE88_00725"
     CDS             complement(11482..12972)
                     /locus_tag="PLE88_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOM75021.1"
                     /translation="MYLMGYDAGTSSIKATLLDLSTGNAAASVSYPPKEMPILSLRPG
                     WAEQNPSDWWENLAAATRLILDKTGADPSAIKAVGITYQMHGLVCVDKAKQVLRPSII
                     WCDSRAVPYGQKAAQHIGPDKCLQLMLNLPGNFTAAKLAWIKENEPDIYRRIDKIMLP
                     GDYLAMKMTDRILTTKSGLSEGILWDFVHRQLAEPVMDAFGFDLDIIPPVVDTFSIQG
                     ELTQQAAAYLGLKAGTPVCYRAGDQPNNAFSLGVLHPGQVAATAGTSGVVYGVADRPC
                     WDKRSRVNTFVHVNHTDQNPRCGSLLCINGTGILNSWLKHHTAGGQDYPQMNALAASV
                     PVGCEGLCILPYGNGAERTLDNRIIDASIHGLNFNVHTQAHLFRAAQEGIVFAFQYGL
                     EIMKQMGLDVRVIRAGRANMFQSELFAKAFADVTQAAIELYNTDGSQGAARGAGIGSG
                     FYKTFEDAFCGLQKIGHIEPDCSRQEAWQQAYKRWLDKLSVQLGRQ"
     gene            complement(13074..14834)
                     /locus_tag="PLE88_00730"
     CDS             complement(13074..14834)
                     /locus_tag="PLE88_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOM75022.1"
                     /translation="MTVLYGVDWLVILGYFAIVFGIAWWAYLKEKKSQTTTEYFLAGR
                     NLGWWVIGAAVFSSNIGSEHLVGLAGSGATDGVAMAHYELHAWCLLVLAWVLVPFYMR
                     SQVFTMPEFLEKRFSPINRAVLSVISLVAYVLTKLAVGIFAGGVVFAVMMPDINFMGL
                     DSFWIGSILVILLTGIYTILGGMRAVAYTSAIQTIVLVIGSACVTFFGLKALGGWSVL
                     REWAGSEMFNLWKPMVPQGVSATWAPVKESTRMAWYFNDNYPWVGMLFCAPIIGLWYW
                     CTDQYIVQRVLGAQNEQIARRGSICAAALKLLPVFIFIIPGMIAFALAKSGMNPALKQ
                     VFFDADGQLIRDNAQQAFPLLVTKVLPMGIRGIVVAGLLAALMSSLAGAFNASAALFT
                     IDFYSKLRPQASQKHVVWVGRVATAVMVLIGLLWIPVIKGGKGLYDYLQGIQSYLAPP
                     IFVVFFLGVFSKRLNAKGCLWALIVGFILGLFRLAVDTPVKMIKGFSYAEGSFFWIVN
                     NMYFQYYSLLIFLVCIGVMVLVSYMTEEPAYDKIKGLTYGTLTEEDRRQSRLSWNKWD
                     VIASCAVMAAIIAAYLYFRG"
     gene            complement(14874..16187)
                     /gene="xylA"
                     /locus_tag="PLE88_00735"
     CDS             complement(14874..16187)
                     /gene="xylA"
                     /locus_tag="PLE88_00735"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOM75023.1"
                     /translation="MKEYFPGIGKIKYEGPQSTNPLAFRHYDPKAIIRGKTMAEHLRF
                     AVCYWHTFKGLGSDPFGGPTIIRNYNSAADPMQAAEQTMDAAFEFFTKLGVDFWCFHD
                     RDIAPEADNLAETNRRLDKIVAKAKQLQKDTGVKLLWGTTNAFSHRRFMAGAGTNPSP
                     HVFAYAASQIKKAMEITHELGGQGYVFWGGREGYDTLLNTDIGRELDHMAMLLQMAVD
                     HKKKIGFKGQLLIEPKPKEPTKHQYDYDVGNCMAFLRKYGLIEHFKMNIEANHATLAG
                     HTFQHELALAAANGLLGSVDANRGDLLLGWDTDQFPTDIYDTTLAMYIILKAGGFTTG
                     GLNFDAKVRRQSIDAADLFYAHIGAMDAFARGLKIAAKMLDDGKFEAALKQRYAGWDT
                     GLGQKIEKGKVGFEELERYILDNGEPELVSGRQELLENMLNDYIC"
     gene            complement(16242..17738)
                     /gene="araA"
                     /locus_tag="PLE88_00740"
     CDS             complement(16242..17738)
                     /gene="araA"
                     /locus_tag="PLE88_00740"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOM75024.1"
                     /translation="MIDLKEYEVWFITGSQHLYGPETLKQVDADSKQIAEALDASAAI
                     PCKVVFKPVLKTPDEITKICVKANTTPNCIGLIAWMHTFSPAKMWIAGLSTLQKPFVH
                     LHTQFNRDLPWDKIDMDFMNLNQSAHGDREFGFICTRLRKNRKVIVGHWKEADVQQKL
                     GIWMRAAAGWNEMRSLKVARFGDNMREVAVTEGDKVEAQKQFGISVNGYGVGDLVAVV
                     NKVADSQIDKLVAEYKDCYKFRVAAEQMESVREAARIEAGLRAFLEKGGFKAFTTTFE
                     DLHGLKQLPGLAVQRLMAEGYGFGAEGDWKTAALVRAMKVMAAGLKGGTSFMEDYTYH
                     LDPKGMKVLGAHMLEVCSTIASGKPSLEVHPLGIGGKADPVRVVFTAAPGAGLNASLI
                     DMGNRFRMIVNTVDVVKPDADLPNLPVARVVWVPRPNLKTAAAAWILAGGAHHTGFST
                     ALTAEHMEDFTEMAGIEYLLIDENTQIPDFKRQMRTNEVYYMLAGSLR"
     gene            complement(17759..18448)
                     /locus_tag="PLE88_00745"
     CDS             complement(17759..18448)
                     /locus_tag="PLE88_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOM75025.1"
                     /translation="MLEQLKCDVCAANLELKKHALVIYSWGNVSGIDRQQGIIAIKPS
                     GVSYDELTPDKIVLLDLDGKIVEGQLRPSSDTPTHLELYRHFSAIGGICHTHSTYATV
                     WAQACRPIPCLGTTHADNFYGPVPVTKPMTRQEVEGDYEKNTGSVIVKTFQKLDPEQM
                     PAVLVANHGPFTWGKTPAKAVESAVVLEQVAHMALHTAVLNPSLIPVADFLLDKHYLR
                     KHGKNAYYGQK"
     gene            complement(18452..20125)
                     /locus_tag="PLE88_00750"
     CDS             complement(18452..20125)
                     /locus_tag="PLE88_00750"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOM75026.1"
                     /translation="MAYAIGLDYGTNSVRCLIVNTQNGKEVGTSIYNYESGQAGILLD
                     PSDHNLARQNPADYLKGIEVTVKKAVRQAKTKDKRFTPEAVVGIGVDTTGSTPLPVDA
                     EGVPLAFKKEFKKNLNACVWLWKDHTGYAEAAEITQLAAKEHPEYLAKCGGIYSSEWF
                     FSKILHCLRTDPKVFDAAYTWVEHADWITAVLTGTDHPSRMKRCRCAAGHKAMFNDAW
                     GGYPSEEFLAKLHPKMAALRRTLPNKTYTISDKAGDLTPQWAKRLGLKPGIPVAMGAF
                     DAHLGGVGSGIQPGTLVKIIGTSTCDMAIAPNDATLPDIPGICGIVDGSILPGFYGLE
                     AGQSAVGDIFNWFVNYIQPGGKTLGSHDALTKKAAKLRPGQSGLLALDWNNGNRTILV
                     DQRLTGLLLGQTLHTRPEEIYRALIEATAFGALTIINRFEEYGVKIEQIVNCGGISEK
                     NPLLMQIYADVTGREMKVSRSAQTCALGSAIAGAVAGGAFADYHSAQKAMCGIKAKTF
                     KPNPKNHQVYQKLYKLYKQLHDGFGLSGNSIGMANVMKELLAIKESSQE"
     gene            20271..21422
                     /locus_tag="PLE88_00755"
     CDS             20271..21422
                     /locus_tag="PLE88_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOM75027.1"
                     /translation="MHKVIIMVDTARASGRKFLRGIERYISSNADWEVCIQPPHYLPG
                     THFNPTSWFKLQEADGLIDRDSNCADAFVHLQIPKIISDTKLENPGVSTIYTNSEKTG
                     RIAAEYFLELGFKHFAYCGFEGLAWSERRFRSFAGVLKKNGFSCVYHYHDWLANAGSS
                     ETEDSRLAQWLTTLPKPVCVFACNDDRGVNVLEACKIAGLTVPEEVAVLGVDNDDLLC
                     DLSSPPLSSIELNFERGGFEAAKLLDQMMTRGMAHTNIVIEPVDIVTRQSTTILAIDD
                     PEVVKALVFIREHYEEPIQVKDVVAATVISRRDLEMKFKNKLRRSIKEEIDRLRIKSV
                     QRKLANTSDTIHNIAASLSYTDPQHFSRFFRKAAGISPSAYRRLNKPYM"
     gene            21513..22250
                     /locus_tag="PLE88_00760"
     CDS             21513..22250
                     /locus_tag="PLE88_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOM75028.1"
                     /translation="MNRRAFLKTMPAIAALTAADLDFAQEADLPSAVSQTSSSIGKLT
                     PIELVKPSADGGKSVLAALWERKTNRSISDRPLSDQDLSNLLWAAFGANRPDGVRGKP
                     GRTAATASNAQEIDIYAVLPAGIYRYDAVAHRLMPAVEGDHRGLVGRRRQGAMAAAPV
                     ILVYVADIGKYKSAPFQEPGLRDPEIQKSYYNVAIGLIAGNVYLFAASQGLAAWFHNC
                     NKPALAEVLKLGPEQYVMYAQTVGHPA"
     gene            22306..23091
                     /locus_tag="PLE88_00765"
     CDS             22306..23091
                     /locus_tag="PLE88_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM75029.1"
                     /translation="MPLFFQSICSSSSGNCLTVWSNTTRLVIDCGLTSMRQTRQVLTA
                     LSAQSKIDSVLLTHTHSDHISYYPLRVLEELGLAIHLHSDCADQLRDIHFNDYGFPKL
                     KLVPFQRKKFVVGDFCIQPFEVVHNPDFPTFGYQIDYQGKKILIVTDFARWEDLIDYF
                     LDADFIFVESNHDLGLLALYFNPNSRFHMSNPDTAKLLVNARRGSRKAPVSVMLGHLS
                     SQRNNAHLALQEIQNAFRNADIELDFHLQTAPLKEASQAILLM"
     gene            complement(23187..24305)
                     /locus_tag="PLE88_00770"
     CDS             complement(23187..24305)
                     /locus_tag="PLE88_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM75030.1"
                     /translation="MNYLADRTQLIDSSGIRKVFALAAELKDPVNFSIGQPDFDVPQP
                     VKKAAIKAIENGFNHYSQTAGEQKLLQRIRDKVKVDYGWDNPPLMVTSGVSGGLLLSF
                     MATVNPGDEVIIPDPYFVMYKHLVRMLGGTCVFADCYPDFKLPIDRLESLITPRTKML
                     VLNSPTNPTGAVYTEQEARAIADIARRHDLLVISDEIYEDFCYDGRCISMGNYYDGTL
                     VLKGYSKSYAMTGWRMGYAVGSEKIKPLIDKMIQIQQYTFVCAPTPFQMAAVAAMDLD
                     ISDYIDIYRRKRNRIYAGLKEAFEVVLPAGAFYMFAKTPAGYPNATEFVKKAIDNNLL
                     VIPGNVFSEQDTHFRIAYTTTEDKIDKGVEILCRLARQ"
     gene            complement(24406..24564)
                     /locus_tag="PLE88_00775"
     CDS             complement(24406..24564)
                     /locus_tag="PLE88_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOM75031.1"
                     /translation="MSVDRSLKIKGALKRHRNVLSREERIQMLKQQERWQEGDKVMGL
                     PKVAHRKP"
     gene            complement(24603..24974)
                     /locus_tag="PLE88_00780"
     CDS             complement(24603..24974)
                     /locus_tag="PLE88_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75032.1"
                     /translation="MPVRKETEVPKPVPIVSEELPRLKPEKGTNVIVIQGISLNRKNE
                     LKPIQDYFKSKGIETEIIENRGYGLLVTKQLFMQDPAIEGTDGYEMKKRIQQLGLLYP
                     AETGDTNFGLKPFQDAYGLKR"
     gene            25977..26990
                     /gene="plsX"
                     /locus_tag="PLE88_00785"
     CDS             25977..26990
                     /gene="plsX"
                     /locus_tag="PLE88_00785"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOM75033.1"
                     /translation="MRIAIDAMGGDYAPAEIVAGALEAKEVLDHSDELVLVGNEDVIR
                     EHLKHNGASPDTFRIFHAPEIIGMDESPVEALRRKPKSSIAVMAHAASHRQVDAVLSA
                     GNTGACVAASQLRMRNLPGVLRPGIAVVLPTLGGPVTMCDVGANIVCKPIHLYQYAVM
                     SCVYAEVMFGIQKPKVGLMNIGEEEAKGTDLVKKTRDLLRSDSTINFVGNLEGRDLFG
                     GKCNVVICEGFLGNVILKLTEGLVDMIFSAIKQELKHRNPLMTLWFKSVMKGLFRKYD
                     YHEYGGALLLGVNGISIICHGSSKARTIKNAILACKKFSTLQINEKIVERLTQSTVTP
                     NGQ"
     gene            26980..27993
                     /locus_tag="PLE88_00790"
     CDS             26980..27993
                     /locus_tag="PLE88_00790"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOM75034.1"
                     /translation="MVNNPTVQTPKRRAILAGTGSFVPSKILTNSDLTKIVDTSDEWI
                     TTRTGIKTRHVVSEGETTATLAAEAARRALNDAGMRADELNLIIIGTITPEMVFPSTA
                     CFVQDMIGAKNAWAFDISAACSGFVYGLSIAQQFVSSGLYQNALVIGAETLTRITNYQ
                     DRGSCILFGDGAGAVILKAANSGPQGVMYSTSFSDGSGWTSLNCPAYGSRHPVGKPLP
                     DPSMVYMHINGREVYQTAVRRIVELVNECLEKCDLDVEDIAMFIPHQMNARIIESVGK
                     RLNFSDDRIFINIDKYGNTSAATIPIARDECRRTGKRKSGDIVLLVAFGGGLTWGANV
                     IQL"
     gene            28027..28965
                     /gene="fabD"
                     /locus_tag="PLE88_00795"
     CDS             28027..28965
                     /gene="fabD"
                     /locus_tag="PLE88_00795"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOM75035.1"
                     /translation="MKTAFLFPGQGAQYVGMGRDIYEQYPQAQALFERAGTILGYDLA
                     DICFNGPDDKLNATEVSQPAIFTVSAAFLELMKASPKAVHPDAAAGLSMGEYTAVYAA
                     GLISFEDGLRLVQKRGQAMQAAAEANPGAMVSILGLDDVQIISLCEEASQGELLVAVN
                     FNCPGQIVLSGTLGACQRAAELAAKYGAIKAVPLSVAGAFHSEMMAPAASQLGQALAG
                     CPIRNPAEIKIIANIHADYYISADDIRQGLVKQLVQPILWQKCIEKLLADSFSSFYEI
                     GPGRVLTGLMKRISRRSQITNISTAESLQEFIGSNA"
     gene            28999..29736
                     /gene="fabG"
                     /locus_tag="PLE88_00800"
     CDS             28999..29736
                     /gene="fabG"
                     /locus_tag="PLE88_00800"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOM75036.1"
                     /translation="MSEQRLAVVTGAARGIGRAIVLELLKQGRKVAGLDLNAQQLAEL
                     EKVVSQSGYSVITREIDITQTDQLTQILEQLAEEHGGIGILVNNAGITRDRLMIQMDD
                     ADFDKVIAVNLRAAFVATRVAARSMVRNKFGRLIHISSVAGVMGQAGSANYAASKAAL
                     IGMSKSVARELGKKNVTSNCIAPGFIMTDMTQVLPEAVKEAAMAVIPMRRFGKPEEVA
                     RAVAFLASDEAGYITGQVLCVDGGMAM"
     gene            29870..30118
                     /gene="acpP"
                     /locus_tag="PLE88_00805"
     CDS             29870..30118
                     /gene="acpP"
                     /locus_tag="PLE88_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOM75037.1"
                     /translation="MKAIEEKVIEIVSEQMGVDKGEITRETSFINDLNADSLDTVELV
                     MEFEDEFDMSIPDEEAEKIQTVGAAIDYIVEIMKNKEA"
     gene            30127..31371
                     /gene="fabF"
                     /locus_tag="PLE88_00810"
     CDS             30127..31371
                     /gene="fabF"
                     /locus_tag="PLE88_00810"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOM75038.1"
                     /translation="MTGRRVVITGLGCITPLAESAEALFDGLCEGRSGISMIESFDTS
                     EYPVRFGGEVKNFDIERYTTARDAKRMDRFTQMALASAIQAVSDSGLNFEQENKHRVG
                     VIVGTGIGGIKEIEDQHIRLLSKGPRKVSPFCVPKLMGNAASGCISIEYGLKGPNICV
                     VTACASASHAIGEAFWNILCDRSDICITGGSEAALTTIGLASFCALKALSTRNNDPQR
                     ASRPFDKDRNGFVLSEGAGILVLEEYEHARKRGAKIYAEMLGYGATGDGHHITAPLED
                     GSGAADAMRLALQQARLNPDQIDYINAHGTSTELNDAAESRAIRSVFGSHAYKLAVSS
                     SKSCLGHSLGASGAIELIACAKSIEKQIIHPTANLEVVGDDCDPKMDFVPGKARQTSV
                     RYALSNSLGFGGHNCSLIIGKL"
     gene            complement(31445..33208)
                     /locus_tag="PLE88_00815"
     CDS             complement(31445..33208)
                     /locus_tag="PLE88_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75039.1"
                     /translation="MRTKAWLISITSAVIGVGFLAAASSRLVPLQQAREKMGLVANPS
                     LENAPPSLAFATVAMGAFRGLIVDILWMRADTLKQEGKFFDARQLAEWITALQPRFAA
                     VWDFHAWNMAYNISVAIPNTQPEERWRWVKNGYELLRDRAIVLNPRSILLYRSLAWIF
                     QHKIGDISDDCHRYYKRELALSMRSLLGENPTNETFQRLASAPKTLDEVLTDPAAARL
                     IAELQQADSAFADAKLLIPNYLTLRRTPARFKKEAFDVIDRYRSTEALRTFDTFARSW
                     QLRNEWKFDIDFMMELNRTYGPSSIDDPNERMPLNWEHPAAHAIYWAALGLKVAGRPE
                     EYRIDEKNTDRIVFHSLQMLYRTGKVVLYDVPNQPPSVYLLPDLRMFESCDQLWKKVI
                     EKYESLEKGNPKAVRGGHKNFLENAVLMFYQAGHQRKAEQIYRRLQKEHLIDESGYTR
                     TEYTGSLMNFLRIRLKSELEGISINDATEFILSVLREGYFRYALHDDDEAAGRENMAQ
                     EIYSIYQQQMGQAEPGRVGLPPMGFLRYQAFIGFLNDPMYPDYMRQSLINRIQLERPD
                     LFDKLRQEEARLLEQMQKEQP"
     gene            complement(33219..34595)
                     /locus_tag="PLE88_00820"
     CDS             complement(33219..34595)
                     /locus_tag="PLE88_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75040.1"
                     /translation="MHSIWAVAQNTLAQALRMKIAVVIILLLGILLPLMSVVMEGDGT
                     LLGKLQTFTSYGLGLISLLLCILTIAVSAFTLSSDLKRKHIFLIVTKPIRRVEYMLGK
                     LLGILLLDTLLLGLFSGVVYGSVLFVYQTAKAPAEQREKAEAEFFTARLGIKAQLDKD
                     QLAKQALARYKELKEAGQIPDGMPYSRVMSELIGQESMKARKVDPGEVKEWEFQNIRI
                     KNTSDPNTLLFVRYKYQVTVTPPDEKVFGMWRVGDIRQFRSGGQFRTPVYRYEEAESI
                     RTVHEFYVPANALAEDGFLGIAFYNNPAMNQTTVIPEELEVLYRTGTFTENYFRAVLM
                     IFIRLMFLAILGLSLSTWLSFPVVILICLIVFLAGLTNGFILDAIESLPAAAGVVYKF
                     TIKPLLWLLPQFDGAYNPNRYIVDGRTMRWMFLAATLGITVCIKGLLMFLAGILIFSR
                     REVAKAVV"
     gene            complement(34588..35835)
                     /locus_tag="PLE88_00825"
     CDS             complement(34588..35835)
                     /locus_tag="PLE88_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM75041.1"
                     /translation="MAADNQFAIETISLTKIFPDWWGRPKVIAVEDLNLKIRYNEIYG
                     FLGPNGSGKTTTIKMLLELLYPTRGAAFVLGGKVSDPKISARIGYLPEESYLYRYLTA
                     RETLDFYGRIFGLPADIRKARIETLLEMVGLSGMGNRPIGTFSKGMARRIGLAQALIN
                     DPDLLILDEPTSGMDPMGTRQMKDLFIELARRGKTILLCSHLLADVEDVCDRIGILYG
                     GRMQVEGTVRQLLQQSDETQIRTGRLSEAAIEQIRRIAASEGQVPEITNPMDKLENFF
                     IRIVAKARQEKQRTSGAEVGTGIQGFLAEKPVVRPESVLQQLVAANVTAPAAPQGPQA
                     AVTKPAPQPQPAAADRDLLGKLVQPVDAGVSQTAVETVQPASAPEKQDAVKRDVLEEL
                     IGKKRDGSTQLTQPVKAEDSKNA"
     gene            complement(35856..37826)
                     /locus_tag="PLE88_00830"
     CDS             complement(35856..37826)
                     /locus_tag="PLE88_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOM75042.1"
                     /translation="MVHTHLGLKAHEMDSCCCDHGSTKSRQLGVSLAFLGTLAGGILV
                     LNSKIAQISWLGGLYGGSTEQNEIIAMIGAVLLGLPIIIHAVRGILQGHMHMDELVAL
                     AVIAAFANGEYVEAGVVAFIMLIAELLETRTALGARASIESLIRLTPTTAMLVDADGT
                     ERQVDVSALKPGDIVRVRPGDNIPADGEVQKGLSTVNEATITGESLPVDKVVGMQVFA
                     GTSNLTGVLDIVVTKAGSDTTLGKVQQLIIAAEQTQLPIMRIIDRYIKWYIPTILMIA
                     AIVYFFTKTLDKSIGILVISCPCALIMATPTAMVAALSAAARLGILIKNVSLLEIAGK
                     ITAVVFDKTGTLTTGQLYVTKLEPAPDVEPVQMLMLAASAEQMSKHPAARAVINLARE
                     ANVSFPQAQTFEEAPGKGVRAVVEGRTVLVGRDSFLKENGIDISNVSDPSLHEEQGFS
                     TLYMAADGKCVGWIGLQDKARPEAQQAVKELFEECSVRRVTMLTGDRSEVANRVSAEL
                     GCTDFKAHCLPQDKLAVVEAIRREGHLVAVVGDGINDAPALAAGDLGIAMGAAGSDVA
                     INSASIALMSNDLRRLPFLVQLSRKTRTVINQNLFFGILFIVFGIIAITVGWVSLIMA
                     AVLHLFGALAVIFNSARLVRFGEHLEPHTQQV"
     gene            complement(37863..39140)
                     /locus_tag="PLE88_00835"
     CDS             complement(37863..39140)
                     /locus_tag="PLE88_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOM75043.1"
                     /translation="MAEHHHHLHDKAKMPQRELPSEPLDASTKSLTQALHVSFSILKV
                     IMIVLLAVFACSGIFQVQQDEEALLLFFGRIQGPAEDPVLKPGIKFAFPEPIHEVIRI
                     PVKREQSLAVNSFWYFETEEEKLRPVKTPVFGPLDPLKDGYCLTRNDRVEGIEGADYN
                     IVHSQWTITYKIDSPKHFFENVYIRDRRPGEDMLEAAAQTLEPMLESMASEAIVRTLV
                     HYSIDEAIKSQTGIADSVKVILQDKLDQIQSGIGIKDVRANRIIWPRQVEEAFQASNK
                     ARQESEQARIDARAYKEKKLTDTGGPDAERILELLTQPGLSPQQQEEYVAKLSGQVQS
                     KISEARAYRTRVVEDAKANAEYLRQLLPEYRKHPELVLQKIYQDAVEQVLAGADEKIL
                     IQPSAEGRSRELRVMINRDPNIKKEKAKQESSK"
     gene            complement(39144..40061)
                     /locus_tag="PLE88_00840"
     CDS             complement(39144..40061)
                     /locus_tag="PLE88_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOM75044.1"
                     /translation="MKNFWIIVSMIVVVVILLLSMVLYQVRETETVIVTRFGKPVRPE
                     TEPGLKFRLPKPIEVVHRFDSRKRLFEDVPEEEITTAGGEPIIVKSYIIWRIADPGKF
                     LISVKDVKSAEENLKSLLRNAQNSVIGRHYFSEFVNTDPEKIQFEQIESEIAGQILQK
                     AMHDYGIEVSAVGIKRLMISKDVTEKVFERMKADRKRKTEAILAEGNAEAERIKSDAD
                     AKQKELLAVADAQAKAIRGAGDAEAARYYKMLEADPELAMFLRDIEALKKILKERSTI
                     VLGVETEPIGLLKGLPDLQPQTAASQTGN"
     gene            complement(40085..42133)
                     /locus_tag="PLE88_00845"
     CDS             complement(40085..42133)
                     /locus_tag="PLE88_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOM75045.1"
                     /translation="MNQSPRKARNTAIGAFCLGLFFFVFTLVFGAFTEVLAMYLLSWQ
                     ILAGLLVWAVLIVQFHQRLLAEQEKMDMTQLSRSAQQGTIFTGGADRLAMLAVAQKRL
                     AFFEKWIIPSAAVVIAVYQIVMGLLLFQYKVLGPVDWRNKNPLLGAVLMIVVSFVSFL
                     FSRYATGMSSEKDWKPLRAGGSYLLLTAVLGFALAVSLALSQFKYPQGLVILSYLIPW
                     LLVVLGAEILLNAVLDIYRPRIAGEYSRAAYDSRLIGLINEPGGILRTVAHTIDYQFG
                     FKVSQTWFYRLLERAILPLILFAALTLYLMSSFAVVGPGQAAVVEYFGSPVRDLEPGL
                     HLKWPWPIEKVYVFPTDQIQQITIGYKEGEKDKAKKAFLWGEKHYEEEYNLLVAVETD
                     TVRDKGAVPVSVVQANIPIQYRISNLRQFMYKHKEARQLLEAICYRELTRFAVSAKIE
                     TDDGSASALPKDRKSLLGVGRMEASQELHQRIQAAADEAQLGVEIVLLGLQGVHPPPE
                     VAKEYEEVVASVQQKQAAILSAQAEKNKVLTELGGSIADVDALYDLAMEFERAKAQAD
                     EPKIQELRQELQAAFVKVRGKVFSTLRQAESDAFERIHLAKGEGLRFAGQIQGYQASP
                     LIYKKLQRLMMLEEALEKARKYVVVAEKDDAQVYIVDLQEKLAASLYDLDLGLEEPKK
                     "
     gene            42308..43273
                     /locus_tag="PLE88_00850"
     CDS             42308..43273
                     /locus_tag="PLE88_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75046.1"
                     /translation="MLLRLLAIAKNTFVETLRQPIYAVIIVFALLLMLLAPAISMYTL
                     DEDIKLLRELGLSTLFLAGLFIAVFASTGAITEEIETGTVTTVLSKPISRPVFVLGKF
                     FGILAAVALAHCLITMAFLMAARHGVLETASDEIDWTVIAAASAAALATFLITGLMNY
                     VYDWNFPSTGVVLGTIFSGFAILFLVFVDRDWKFNPQNNRFEMFDIAASALLLMAMSG
                     LVALAILFSTRFNEVITLTCCVGIFLLGLISDWIFGRFASQHLWAKIAYILTPNLQIF
                     WVSDAIYEKGAVPAAYLLQTASYTALYTAGILALAIALFQRRQVG"
     gene            complement(43461..43796)
                     /locus_tag="PLE88_00855"
     CDS             complement(43461..43796)
                     /locus_tag="PLE88_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75047.1"
                     /translation="MKSLWNVHYKSPTLKTPLSCQPATNGQVRKHQTEASKSKKQQKR
                     YTINKGLLLISTLLRNVAAACRITPSIISDLQKRQLNVQRKPSSIEGKWLLWLSENGD
                     AVFQSLTIS"
     gene            complement(43768..44220)
                     /locus_tag="PLE88_00860"
     CDS             complement(43768..44220)
                     /locus_tag="PLE88_00860"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOM75048.1"
                     /translation="MLIRDAQIKDVKRIHELINSYAEVDRMLFASVSDIYERLLTFMV
                     AEEQGRVVGCCAIQVLWENLAEIKSLAVDPAYFGKGIGKALVLGCLDKARRLGLKKVF
                     TLTLEDKFFEKLGFVRVQRDKLPMKVWSDCAACPKQDHCDEVAMECTL"
     gene            44349..45767
                     /locus_tag="PLE88_00865"
     CDS             44349..45767
                     /locus_tag="PLE88_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOM75049.1"
                     /translation="MQTEKQYRLNLHPAALNRTIAVLAAPAIFENLLFSMVFFADTLI
                     VGWLRNENYLAATALASLTMFWTNAPIQAISIATVSIVSRTLGENDYASARRIAGHCL
                     ILTFAAVFAILAVGFPFAEWIIRLLNAEPPVIPLAAAYLRIVLVSSVFGLPLMVSNAV
                     IRAKGDTATPMFITLIMNLINIIASILLAFGWGPIPAMGLYGVAWGTVLARNIGGALS
                     LAVLISSKHGIGLHIRDFVPLHRRIFARIWHVAYPAIAERVLNSTSYAFFMGMVASLG
                     TTVLAAHQIALNVESLAFMPAFGMGVAITTIFGQAVGSGRWRIGQITVKRAIIFSGTA
                     GILLSLLFALAAPYGVKVFRATPEVLRLAGLALQIAALELPLFTLTFVFMGALRGAGD
                     TRSPLYVNVACILLLRLPATWLLAFVLKMGIAGVWIATALDWAGRSLGLGLVFRRGIW
                     KKIHHLEKQKFAETAIPVSPEP"
     gene            complement(45795..48188)
                     /locus_tag="PLE88_00870"
     CDS             complement(45795..48188)
                     /locus_tag="PLE88_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75050.1"
                     /translation="MTRMKTVFVVYLLTLHAAASWGQLNTSQPHIGYLYPAGAKQGST
                     VLVTAGGQFLRGADAVYISGQGVQGRVIRFYRPLGNLQKEQRNLLGSRLDEVRRKRIE
                     ELTGKAAADDAAAKPSPQATASEDKPNKEAPPVQMPEHPLLYKLEQKNLRELAHIRSM
                     LFFPRTKLQQNRQISETVLLEITAASDAPPGRRELRLSTSAGLTDPITFEIGTLQEVR
                     ELEPNNRQPYPPLPDVPDAPAEKPLELPVLINGQIMPGDVDRFCFTAPAGMQLVAKVH
                     ARSLIPYLADAVPGWFQAVIKLYDSAGRELVYADDYSFNPDPVLFFTVPRSGEYELEI
                     RDSLYRGREDFVYRIAVGPLPFITQIFPLGAREGESAACQIKGWNLPADRMPLDTSPC
                     AGCIRQLQCQGSAGVSNEIPYAVGTLPECTESLSTLGDAQPVELPVIVNGQIEQAGRA
                     DIYCFDGLAGQEIAIQVCARQLHSPLDSLIRLTDASGKVLAWNDDFAVWGEGWLFMDS
                     TGLLTHQADSYVTAVLSSSGTYYVHLSDAQQHGGAAYGYRLRISGLMPDFAVRMTPSS
                     LTVPAGGVAAVRFYALRTDGFEGEIEIVLKDAPAGFEISGGRIPQGRNSVWATLSAPA
                     RKLDKPVSLRLDARARIGGSIITRPVVPAEDRMQAFLYRHLLPSQELLAAVPRAGQRM
                     SAIKPDGQIPMKIPAGGSTEVKLKLPPRLAQGNNIQLALHEPPAGLTLQEVKSKASEI
                     SFVLKADRELLKEGFADNLLVEAYTETPAAGTGSPAAQKQRISMGAIPAIPFEIIPP"
     gene            complement(48201..49514)
                     /locus_tag="PLE88_00875"
     CDS             complement(48201..49514)
                     /locus_tag="PLE88_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOM75051.1"
                     /translation="MNRQRIKKGLTRRDLLKQGLAGAVSLHCSRLWANPPAVPKARAQ
                     SVIQIWMWGGPSHLDTFDPKPEAGYDYCGPLNQPIPTNVDGIQISQLLPLLAKQADKY
                     AIIRSMTHGNNNHETASYLVQTGREPGRLVYPSVGAVVSLFKGYDHGYTGIVPPYIVL
                     TESQGRFSEAGFLGQRYKPFVTGGDPNQTPFAVEGIVAKGISDQRQQNRRQFLHSLDV
                     LGKLMPDDPQFARLDQCEQNAYAIMFGPARRLFDLSEEKEEVRQRYGRNTFGQCCLMA
                     RRLVEQGVPYVTINYKGWDTHKQHFDIMRRKLPELDMGMSALLQDLADKGLLDTTIVW
                     WGGEFGRTPKIQWEAPWNGGRGHYGKSFCSVVAGGGFKGGCVVGASDEKGLQPAQRPV
                     YPQDLIGSIYLLLGINPDGPLPNAGGLDVKVMPESSEGGRLREIM"
     gene            complement(49548..51176)
                     /locus_tag="PLE88_00880"
     CDS             complement(49548..51176)
                     /locus_tag="PLE88_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOM75052.1"
                     /translation="MDMYKKLDKYEVVRSKPLFTMLAAVWCIAGVYGASSQQSNQQND
                     KVSIFERSSDFRPVNEIDSCILSELSKRGIKPANLCSDSVFIRRVHLDIAGTLPDAQE
                     VRRFLENPRSDKRAALINTLLESDEFAQYTALKWCDLLRVKSEFPINLWPNAVQAYHR
                     WIYDALAANMPYDTFARQLLTSSGSNFRNPPVNFYRAVQDRDPSAVAAAAALAFMGVR
                     LEKWPQERRSGIEVFFSQIGYKGTAEWKEEIVYPKPAESGPLTGVFPDGQPAVIQPNQ
                     DPREVFANWLIRADNPWFARCAVNRIWSWLMGHGIIDPPDDIGPDNPPVYPDVLAFLE
                     KELVRSGYDLRHIYRLILNSRTYQQSCIPQAKSPDAQRLFACYPVSQLDAEVLMDALC
                     QITGTQESYSSPVPEPFTFIPDENRSIALADSSITSPFLELFGRPARDTGLQSERTNL
                     PTDAQRLHLLNSSHIQNKIERGPHLRQLMRAAGRNRRMLVDTLYLTILSRFPEQTERS
                     IIEDYFNTQGLTPAQAVNDLVWALINTKEFLYRH"
     gene            51470..52555
                     /locus_tag="PLE88_00885"
     CDS             51470..52555
                     /locus_tag="PLE88_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOM75053.1"
                     /translation="MKITDKLHSIRISGPSGAEYFLHAQNRFPAQAAELLAELSAFLQ
                     QTDAQLVYLRIFAQENVRTTLLSSCLSQLQRLCCPIIWIRQDNNNYAFGLQGYAVEGT
                     DTAPIMVDNRLAGRCFTSGGCRYYHFSFTPQNPDAGRFEQAAALFAQMQKTLSSAGLD
                     FSSAVRTWLFAEDILSWYSQLNQARTAFFTEHNIFQKLVPASTGVGIGNLLGGAIAAE
                     LLAVKPLNGCVRIEQKASPLQCEALAYKSSFSRAVLIASVSDRRLIVSGTASIDQNGL
                     TVWPGDCAKQIEQTMQVVHALLDSSGMDWNDTVRAIAYFKNSADFGLFDAYCQSRNLC
                     LPHLKVQADICRPELVFEIELDAVRCC"
     gene            complement(52581..54125)
                     /locus_tag="PLE88_00890"
     CDS             complement(52581..54125)
                     /locus_tag="PLE88_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75054.1"
                     /translation="MKAKYHFFATVYFLWIVLACLLTAAAFADEYPDSFNNQAAASGA
                     SVLLKNGPVRYRLIEGSTLLDECLFCDRLSVPVPIRGTFWLVPKEPDMWFLHFAVRNL
                     RFNSSRTDVRYFGLLEGDYEIGGDFAFVHRMTLQGSINNNQGLSFDSGVVFPTMGLPW
                     IEIELEQCKPDDPLHVFRLHLTAVPWPQVGFSTAAGFHPSVRTVLDKTYISDGDLLTV
                     HGRILRTNAQLTSRLGIMPLVPDVGLDAVIGARWPQLTSRDCKCRTFWFSMEQDIFSE
                     ILGPLHQGDLLSEAGFIVRRFTDFIRPFCPQPPVPDYGLDAVTFSPSGRLLFSVEKGF
                     FSESLGVMISPGDLLSEDGRIYRTNAQLLKQFETAQSSVGEYQDVGLDAVYVWPHGEI
                     WFSTEKDFADKRFGWIGHGDLLSDTGRIIARNRELTAPFKPIEDLADFGLDALHIVWP
                     ILRADINGDGIVNLDDFAAFSNYWSLRTDNAADVCDYNIDLSKDYAVNAQDLTVFADD
                     WLLCAE"
     gene            complement(54320..55549)
                     /locus_tag="PLE88_00895"
     CDS             complement(54320..55549)
                     /locus_tag="PLE88_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75055.1"
                     /translation="MKVWHESVKPSQKSTILVTCGPGLVGYLRSEIEQLGYTITAAGS
                     VDAAIEGDMFDAMRLNLYLRTAYNVLYLLRSFPCDTPDELYRKIRQMPWEELIDPAEY
                     FSVVTRVDTPSIDNTMFAGLRVKDAIADRILKQCGSRPNSGKERDNVVIQLFWRGKTC
                     KVYLNTSGRKLSDRSYRKTMIDAPMRESLAAAIIMAVGYDGTQPLVCPMCGSGTLPIE
                     AALTASGRVPALLRSNFSFMHTKWYDALAWHALRAEAGKKSRKTGGKAQHRPAPIIAT
                     DIDSWAVEAARRNAQTAGVEHLIEFSVCDFAQTPVPQEPGIILMNPEYGMRMGDVKEL
                     EETYARIGDFFKQKCIGYTGYIFTGNLELAKKVGLRTSRRLTFFNGGLECRLLKYEIY
                     AGTKKKSALEPDSSAGN"
     gene            complement(55597..56670)
                     /gene="ychF"
                     /locus_tag="PLE88_00900"
     CDS             complement(55597..56670)
                     /gene="ychF"
                     /locus_tag="PLE88_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOM75056.1"
                     /translation="MKVALIGLMQSGKSTLLSAISGKEPAPMGSPDIHEHIVSVPDDR
                     LDWLTELYQPKKTVHATVDCLDLPGLSFADEHGRAAARRLFGQIRTADMFVLVIRDFD
                     SPSVPAYRGSVDPVRDLEELKTEMLLADLEMVTTRIDRLQKQAHKPTKTQAHDKAELE
                     LQLKLQAAIEAEKPLRSVLQESDGPQMEMVRSLNFLTLKPLMVVVNVGENRLGQNGGS
                     SIGIEGVPVIRLCAKLECELAKLDPDSRADFMKEMGLAQPASKQFVQCCYSTLGLISF
                     LTVGKDEVRAWPIEKGTAAVDAAGKVHSDIKRGFIRAETISYEDLRRLGDEKAVRAAG
                     KMRLEGKSYIVQDGDIINFRFNV"
     gene            complement(56694..57263)
                     /locus_tag="PLE88_00905"
     CDS             complement(56694..57263)
                     /locus_tag="PLE88_00905"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOM75057.1"
                     /translation="MKNRAESYSQNHMTNKKIITTPLDNAVVQHLHAGDEVLICGTVF
                     AARDQAHKRFCDCIAQGRPLPAAIEGALIYFVGPTPAPPGKIIGAAGPTTSSRMDAFT
                     PMLLAYGLKGTIGKGYRSQAVRDALVKYGGVHFSALGGAGALLSRHIVRNEIAAYEDL
                     GPEAVRLLEFKDFPAVVAYDCWGKSVYDR"
     gene            57378..58019
                     /locus_tag="PLE88_00910"
     CDS             57378..58019
                     /locus_tag="PLE88_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOM75058.1"
                     /translation="MAKYPIYLELSGRRAVVVGGGAVAVRKVQALQEAGARVIVVAEY
                     VHPSHADALRLPNVELIISPYRKDYLAGSTLAIAATNDPALNEQIFHDCQELEVLCNV
                     VDKPELCDFYTPAVVKRGDLQIAVCTEGDCPAYAGHIRRKLEELFTETHGHFVKQLEK
                     VRQQIIMEIPDADQRKSLMGLLASDESFEIFRHQGQQKWNAYVLAQLAGSKTA"
     gene            58051..58467
                     /locus_tag="PLE88_00915"
     CDS             58051..58467
                     /locus_tag="PLE88_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOM75059.1"
                     /translation="MKCLTREIWMNVPQRRAIVSIHRQIEQLVEESGVREGLVLINAM
                     HITASVFINDNEPGLHHDYEVWLEKIAPEKPYSQYQHNGCEDNADAHLKRQVMGREAV
                     VAITAGKLHLGPWEHIFYYEFDGMRKKRILVKIIGE"
     gene            58467..59102
                     /locus_tag="PLE88_00920"
     CDS             58467..59102
                     /locus_tag="PLE88_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOM75060.1"
                     /translation="MTELLLFLIQAVIISLSGVMAPGAMTAAAIAQGTRNRWAGILIS
                     IGHGAVEIPLIFLLMLGLHFLFEMPAVQKVVGLLGGAFLLWMGYGLLRQSGQPRTPQH
                     PSGHSALSSIGAGIVLSAANPYFLLWWATVGLNLALGAKNLGFAALVLFAAVHWLCDL
                     VWLFLLSFAAFYTNKRAGLWGRPAEKAILLFCGSALLLFGIKFIYSALMPA"
     gene            complement(59099..60553)
                     /locus_tag="PLE88_00925"
     CDS             complement(59099..60553)
                     /locus_tag="PLE88_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOM75061.1"
                     /translation="MSYEPVFIQKLRQLFTLPTQGLGKGSRFLALQVRVWFHCFRLLG
                     VNHCLTQAAALAYHSIFGLVPLAIVILMIFQMFPANREAGERVKKLLYESLNLTHIEY
                     PVEETELPPDGSGPAAAAAGSPVPAAGGKITVAAKLDELIERYMSKLNTGAITGVGLV
                     LVIWAAIGLLTTIERTFNTIYHVPTGRSFLHRLFNYWALLTLGPLLIGAGLYVSARYL
                     LVHEFSQGIFAFLGPTIPFAISIVGFFFLYYFLPNTRISPGAALWGAFVATILWTAAK
                     YGLRVYMSKVVLYQAIYGILGIIPLTVFWIFLSWLIVLFGLQLTYAAQNIKHLDAAEF
                     QKTRRRQQYFLANDQTVIRLMQYVLNAFERKDQKPVSVEAVAWRFGMPVDFAGKILDH
                     LVHSGLLCHTIEPSVGYVPSTDGAHIKLDEISRSISEVSFAQPGSEETAMQQVFEEMQ
                     SHLSRYTLKQVLAKEERFDYQADKQTDAGSGTSD"
     gene            complement(60550..>61833)
                     /locus_tag="PLE88_00930"
     CDS             complement(60550..>61833)
                     /locus_tag="PLE88_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75062.1"
                     /translation="GEKTISADASAQIDLAKAWPFAQVFAEVPEGMQIAGLLDATLKA
                     ATAGSQMRLQSENTKVDGLKIIPKAGEPFIQDRITLKCDIVLDTEQQTIDIGAFDLKG
                     AAGQTLISITKGEVKKKVSSSLTRISGDVEAHYDWQALSAFASMYLPEGLSVKGARKD
                     TFHFDSEYPTDKPELMKANLNAAGSVGFDSASYFGLNFGPTDVKLNIVKGLVDLTIPQ
                     TTVNGGKLQFAGTMNLAEEPLLFRLKQPMTILDKIALNDTLSQAMLLYTSPIFANAVK
                     TSGILSFSSQQMVLPLSGKDMMLLTLKGTFGIESLRMQAGDFLGQLLTLLNAGDSVML
                     TVRPTDFSVEKGVVSYKDMQVDVGNTPLHFRGKVGLDKTLTMDVEIPVDKNTVTVPIE
                     GSLLRPKINPAKLIEKQGQQLLEKEIQRGLERIFR"
BASE COUNT        14827 a        16980 c        15216 g        14810 t
ORIGIN      
        1 cggaaacgac gagaccccga ggggatcacc ccacggggtc ttgcgttaaa caagcaccgc
       61 tgaaatcagc ggatggattt ttggctcccc gacgagggct cgaacctcga acctagcggt
      121 taacagccgc tcgctctacc gattgagcta tcggggaata gatgtattct gatacccagt
      181 taaaatcaga agtcgtgatt ttgcacaaaa tcaggacttt tgtcaagaaa ttccttataa
      241 aaaatacaaa tttctcaaaa aaaaagaccg gctatggaca agaaacacca aatcatctaa
      301 aattgtattt accccggggt aaataaactc ccagatacat ctaaaatgtt aaattgattt
      361 tgacgatagt aaattggtta tggttaaaag tacaattgtc gatatagatg gatatacaat
      421 tcttcgccgg ctgggtaccg gtgcacggtc tactatctac ttagctgtcc aagaagattc
      481 caaaacaact gtggcaatca aaagggccat tcttgaaagc ccggaagaca cacgtatttt
      541 cgaacagatg gaaacagaat acaaggtctc ccggcagatt gaccatccgt atatacgcaa
      601 gtgctacgat attattcgca atcgcaagct gcttcggaca aacgagttaa tattgattat
      661 ggagtatttt gagggccaaa atctcgagga tcagcagcga ttgagtatgg gagacgttct
      721 gctggtcttc cgaatggtag ccattgcgct gaatgccatg cacgaaaaag gatatgtgca
      781 ttgtgatata aaacccaaca atattctctt cagtcccaaa gggggcatca aaatcattga
      841 tttgggacaa agctgcagac tgggtgaaat caagaaacgg attcagggca cacccgatta
      901 catcgcaccc gaacaggtgc gccgtgaaca tttaagccac cggactgata ttttcaatct
      961 cggcgctacg atgtattggg ccttgaccgg ccggaatgtg cctacgctga ttccaaagaa
     1021 aaatgagatg ggcattgcaa ttgatgaaac ggcatctttc aaatcgccca atgagctgta
     1081 tcggaaaatt ccgaaaaccc tgtctgattt ggtaatggaa tgcgtacagg agaaacctgc
     1141 agaccgtcca tccaatatgt cggaaattat tgaccggctg gatgtgatga ttcgtgaaat
     1201 ctttgggtcc cgaacatccg gcaatgcccc aaaagtcaat acaactctgt cgtgaagggg
     1261 ctgaactcaa ccgctcctgc cgtttccgca gcggcaatgt tatccatctt ccggatatag
     1321 gcaaggttat cgttgcaggc gatctgcatg gccatcggcg caactttgaa cggattcaaa
     1381 agtttgctga ccttggaaat aatccatgga ctcatgttgt cctgcaggaa atcattcacg
     1441 gtggcccaga ggatgattac gggggatgtt tgtcatggca gcttttgttc gatgccatcc
     1501 ggtataaaat ccgctttccc gaccaagtac atattctttt aggcaaccac gataccgctg
     1561 tcatcagcaa cagctctgtg ctcaaatccg gcaaggaaat gaaccgggct atgcaggcgg
     1621 cgatgcgccg tcagttcgga caacattatg acagcgtcta tgcggcgatg atagagtatc
     1681 tgctgagcca gccgctggtt gtccgctgtc ccaaccggat atggatcagc cattcgctgc
     1741 cggctgacga ttatttggaa acatttgact tatcgatttt taaccgcccc tataccatgg
     1801 aagacattga acggcccaat cctgtttatt tgctgacatg gggacgccgg cattcagaga
     1861 atacgctcct gcgattggga caaatgctgg atgcagatat ttttatactg ggccatcagc
     1921 cgcaggagac aggctggtca atgggcggct ccaataccct gattatcgca tctgaacacg
     1981 cctacggctg cattttagaa tttgacctga gtaaaacgta tacggcctca gatttggcca
     2041 gccatattat tccgctggga accattgaat aagcaggcct gcggcaacag cagctttatt
     2101 cctcggatgt atcgagaatg gacatgaaag cttcctgcgg aatattaacc atgccgatcg
     2161 atttcatccg ctttttgccc tctttctgct tcttgagcag tttcatcttg cgtgttacat
     2221 cgccgccgta gagcttggcc gtcacatctt tgcggaaagc cttgatggtt tcccgggcaa
     2281 taatcttgcc gccgatggct acctgcagcg gaatctcgaa ctgatgtcgc ggtatggctt
     2341 tcttgagctt aagaataagt tttcgccccc gctgttcggc tttggtgcga tgcacaatca
     2401 gcgaaagtgc atcgacggga gtaccattga ccaaaatgtc aatacgcacc aagttgccgg
     2461 cctcaaagcc cagaaattca taatccatcg tcgcataacc ccggctgatg cctttgagaa
     2521 catcataaaa atcatacaca atttcggcca gcggcgcttt gtactccatc atcacacgcg
     2581 ttggactgat atattcggtc ttcagcagta cacagcggcg ctcttcggcc agtttcataa
     2641 taccgccgat gctttcgcgg gtggtgataa tttccagctt aaccatcggt tcgcggattt
     2701 cggcaatttt ctgacgatcc ggcagctcgc tgggtgaatc gatgcgtttg agctgcccat
     2761 tggtcatcag aatttcatag ctgactgtcg gggctgtctg gacaacatca atatcacttt
     2821 ctctttccag acgctcctgt acaatttcca tatgcagaag ccctaaaaag ccgcatcgaa
     2881 agccgaaacc cagagccgga gaattctgcg gcacaaagga aaaactggca tcgttcagcg
     2941 ccagcttttc aaatgctgcc cgcagacgcg ggtaatcggt agagccgccg ggataaaaat
     3001 ccgaaaatac catctgcatt ggtggtttgt agcccggcag aggttgggct gccggttcgg
     3061 ccgcatcggt aattgtatca ccaatcgtaa catccgagag gttcttaata ttggcaatca
     3121 catagcctac ttcgccgcag ccgagctgtt caaccggcgt catccgcggg gtgaacttgc
     3181 ccagttcgac aatggtgtag ctgcgtccgc tgcccatcag ccggatgcgc tggcgtgttt
     3241 tcaggctccc ttcaaaaacg cggacatagc agataacccc gcggtattcg tctgcatggc
     3301 tgtcaaaaat cagcgccgcc gtcggacagt cggatttatc ctgcggcgga ggcagccgct
     3361 tgacaaccgc ttccagcagc gcatcgatgt tttgaccggt ttttgcactg atgaaaatac
     3421 actcggagcg gttcaaaccc aaaacatgct ctacctcttc agccacccgg tcgggctgcg
     3481 cggcaggcag atcaatttta ttgataacac caactatttc gcagccgctt tccaccgcca
     3541 gataggcatt ggcgactgtc tgggcttcca ccccctgact tgcatcaacc accagcagcg
     3601 caccctcgca ggctgtcaat gcccgcgaaa cctcataatg gaaatccaca tgacccgggg
     3661 tatcaatcag gttgagcata taaacagtgc cctgataggt atattgcatg gttacagccg
     3721 aggccttgat ggtaataccg cgttcgcgct caaggtccat atcatccaga agctgctctt
     3781 tcatctcgcg ttcagagata gcccctgtgc gctcgagcag ccggtcggcc aaggtgcttt
     3841 tgccgtggtc gatgtgtgca acaatcgaaa aattgcgtat ctgttgtatc ggaatcggat
     3901 tattctgcat tgggggaata tatcacaggc ggcggctttt ttcaagtccc atcctctatc
     3961 agacctttct ttccgccggt cttctgccga tgcccggaag tatcctgtgc gatctgcagc
     4021 cggcgaatct gtgccagcaa tttgtgtttg ccgcgggggg aaagaaaggc ctcaattatc
     4081 ttaaaatgcc gcttgtcata atactgcaaa agggctatct gcagccgttt ttcagcgcgt
     4141 cggcgaggaa tatggatggc ctttccggct ggtgaaagac ccgacacata catcgccgtt
     4201 gacagtgcca gcggcaccgg gacaaaatcc tgcacctgcc gcggccgcca gccgcgccga
     4261 accaagtact cggttagctg caaggcctct tcaagggaac agcccggatg actgctgata
     4321 aaatacggca caagaaactg ttcttttcct gccttgcggc tggcctgaac aaaggctttt
     4381 tcaaatatct caaacgtttc aagatgcggc ttctgcatca tatccagcac ggattggctg
     4441 atgtgttcgg gtgccacttt cagatgtccg ccgataaaat gagctgccag cagttcaaga
     4501 tattcggagc tatgcaatgc aaggtcatgt cggatgccgg aagcaacgaa aatatgcacc
     4561 tttttctttt gtgctttctg ccaagccaga atctttttaa gcagcgaaac aaccgcacga
     4621 tgcgaattcg gcagcttggg acacggttgt gggtatagac agctcattcg tttgcatttt
     4681 gctgccttgc agcaggccat tccatacatg ttggccgttg gaccaccaat atcttgtata
     4741 gttccatgaa attgcggatg ccgaataagt ttatccagtt catcaagaat agatgcttcg
     4801 gaacgactgg caatctgctt gccctgatgg aaatagatcg cacaaaagct gcatcccccg
     4861 aaacatccgc ggtgggtagt aatactgaac tgtatcggct tgagtgcagg aatacccccc
     4921 tgtttgtcgt atttcggatg ccagcagcgg gcaaacggca gcgaatacag cacatcgagc
     4981 tcctgctgac tcattggatc ggctggcgga ttaaccacaa tcagcccggg cgtttggtcc
     5041 tgtatgacgg gtctgcctgt ctgaattgcc tcttgctgat atgcgagctg gaaattcata
     5101 aaaagctgat tattcttttc aagttcctgc agtgaaggca aacaaacagc atctgggggt
     5161 atcggtgcac ttccaactac gcgatatgcc agacccgcaa tgtctgtcat ttcattaata
     5221 gacatcccag aatccagacg gcgggcaact tcgaggatag tccgttcggc cattccataa
     5281 accagcagat cgcctttggc atcggttaaa acagaacgtt tgagtttatc ctcgatgtag
     5341 tcataatgca ccagccggcg cagactggct tccagcccgc ccaaaataat cggcactcct
     5401 ttataggcct cacgggcacg agctgcataa gccaacaagg gacgttcggg gcgccggcct
     5461 gtttttcccg ccggactgta cacatcctgc cgccgcttgt tgcccaaaga tgcataatca
     5521 ttcaaacggg aatcaatgca tcccgaactt atgccccaga aaagccgcgg ccggccaagc
     5581 gtgcgaaacg gctccacact gcgccaatca ggctggtcaa ggacagccac tttaaaacca
     5641 tggtactcca gccaccggcc tatcagcgct atgccaaaag atggatggtc cacatacgca
     5701 tctgccgtaa ccagaataat gtccggcctg tcccatccaa gagctgatat ttcctgaggc
     5761 gttgttggta aaaagacgct tctcatagca aaaacgcaat tttcggtatt ttttcaaggt
     5821 tgacaaataa tttagtttac actacgatta ggtatccgtc gagtaggata attgtattta
     5881 ttcatcagaa acggtaagaa ttgaaatttc gggttcattt tttgactcgg aatatcttaa
     5941 tattgatggt ttcaatcaga ataggggcct gtagctcagc ggtagagcag gggactcata
     6001 atcccttggt cgagtgttcg aatcactccg ggcccattga tgctgtattt gagcaatgac
     6061 aagccctaaa aagagagtgt ttgactggat ggtagtaccg gttcagaagg tcacgtggac
     6121 ggagggttgt tttatctggc ctgtctgtcc tcttcttgcc agtccttaca gtgctgatac
     6181 aactcccctt gcccagcttt taggcgacct tcagcggcat ttacacattc ggggatatct
     6241 tgtgcggaat gcatttggcc cggcagcaat gcgcatccag cgcgattcat ctatccgtca
     6301 tcccgaagct tatcgtatgg tcatagagcc ggatgccata actgtaattt ctgcttcgga
     6361 tgcaggggca tattacggcg ttcagaccct tcgggatatc gtatatttat gcggcaggca
     6421 gataccttgc tgtgttatag atgaccagcc tgtcttcagt cgccggggcg tctatcacga
     6481 ctgctcgcgg ggcaaggtac caacactcca tacgctcaag cagctggtcg agcggttagc
     6541 ccattggaaa attaacgaac tgcagctgta tgtagaaaac gttttcacat ttgtccgcca
     6601 tcccctcatc ggtaagggct gcagtccgtt taccccggag gaaattctgg cgctgcagga
     6661 ttggtgcagg cagcatcata tcaggctagt cggttcattg gccagtttcg gacatcttga
     6721 aaaaatcctt tgtctgcctc cctatcggca cttgggggaa atgtacggtt tagacaactc
     6781 ctccggcagc accactctat gtccgatcga tccgggttct atcaagctgc tggcggaact
     6841 ttacgaagaa tatgtccctc tttttgaggc agaggatttt aatgtctgct gcgatgaaac
     6901 aacagaatta ggcaaaggca gaagcaggcg gcgagctgac aaaatcgggg caggacaggt
     6961 atatctggac ttcgtgctga acctatacaa actttgtcag cggtacggca aacggatgaa
     7021 tatgtgggca gatattctgc tggaacatcc tgaattactg gataagctgc cgcacgatat
     7081 tgtcctgctc aactgggaat atgaagccga cggcaccaat atccgacaaa cccccaaaat
     7141 agcacaaaaa gggctgccct tgatggtttg tccgggcacc agcagctggc ttacacacgg
     7201 ctcacggctg gataatgcca tgcgcaatat cgccggtttt gcccagcagg gccgccgctg
     7261 tcatgcagaa ggcctgctta atgccgactg gggcgatcgc ggacaccgaa acctgctggg
     7321 catcagcctg cacggttttg cctatggagc agcctgtgca tggaatccgg cagacgcaga
     7381 caacagccgc ttcactgaac atttctgccg gctgacgttc ggacagtgtg aaagtcatct
     7441 ggccaaagct gtccgcattc tgggcacaac ttaccgcacg tgcggagccc cgcggcggaa
     7501 tggttcattt ctctttgaag gaatgactga gccgatgccg gcttcggaag ccccatccag
     7561 cagtgcgata gataaaatga ccagcgccgg actgaaaaaa attgtcagtc agctttcatc
     7621 ggaccgctta tggccaacag cagcagcttc cctgcccgag tttgaacgcc ttgctttggc
     7681 agaattgaag ctggcagcaa agatggacct ttggtctgcc cagcgggcac tggcagccaa
     7741 aaacctccgc acaggtcaac ctgtttcagc caaacagatc cgccttctgg ccgagcaaat
     7801 gaagcaaatc gaagcggaat tttcatctct ctggatggct cggtgcaagc cctcccgtct
     7861 atgcgacaat cttcgactgt ttaagaaaac gcgtcagcaa atgctgcggc tgatcccgca
     7921 ttaacagaga ctcccgatga acaaacccaa atcgattgca gatcttgcca aacgaaagca
     7981 tctccgagca gcaggactga tgtccggcac cagcgcagac ggcattgatg ccgcaattat
     8041 agacatacat ggcaaaaaca tatcggttgc cggatttggg accgtgccct attctcccgc
     8101 ggttcgaaca gccattcttg atctctgtaa tccggcagga tcgcggctgg acaatatctg
     8161 tcactataat gccctgctgg gagaactctt tgcgaaagca ctgattcggg tatgtcgaaa
     8221 agaatccatt ccgcttgaca gtatcgacct gattggttct catggccaga ctatttacca
     8281 caatcccgcc ggccggcgtc aagccggacg tctggttcgc tccaccctgc agattgggga
     8341 gccgtctgtc atcgctgaac ggacaggcat actcaccgtg gctgattttc gcccacgcga
     8401 tatggcctgc ggcggacaag gtgcgccgct ggtgccgttt gccgattatc tgcttttcag
     8461 ccatcggcga cagaaccgga tcattcagaa catcggaggc atcgccaatg cgacctggct
     8521 gccggcgggc gggcggcctg aaaatactgc ggcctttgac acaggtccgg gcaatatggt
     8581 tattgacggg cttgtccgga tcataaccgg cggcaggcag aattatgacc gcaatggcag
     8641 ctgggctgcc cggggaactg ttcaccaaga ccttctgtca gaactgctca cacatccata
     8701 ttttaaccgt tatccgccaa aaacctgcgg acgcgagcag ttcggtctgg attactgccg
     8761 catgcttgtt aaaaaagctg cccggctgcg attgaacgaa gcggaccttc tggcaacagc
     8821 caccgcctta acggcctttt ccattgcaca ggcataccgg cggtttcttg acaaacagcc
     8881 ccagcaggtt attctatgcg gcggcggata cagaaatcga acactggtta aaatgctgcg
     8941 ctggtacctg gaagatatgg aacttttaac aacaaacgat tttggcattg atgccgacgc
     9001 caaagaagcc gtctgttttg ctctgttggg ctgggcagcc gtaaaaggca tccccgccaa
     9061 tattccctcc gccaccggcg cccaccgcgc ggccgtttta ggaaagattg tcccgttatg
     9121 aaagaccgat caaaactgac aaccgaacag cagaatatac gaagccgcaa catcgacagt
     9181 ctaacaaccg agcagattgt ggcagtcatc aatgccgaag ataaaaaggt cgctcccgct
     9241 gtcgccagag aacggaaggc aattgcggcg gcggcagata tgatcgccgc ccgtttagaa
     9301 cagggcggac ggctgtttta tgtgggcgcc ggaaccagcg gacgactggg cgtattggat
     9361 gcagcagagt gtcctccgac ttttggagtc cggcggacac tggtgcaggg catcatcgcc
     9421 ggcggccggc gtgctctgac acgctccatt gagggggccg aagacagcgc tgccgacggc
     9481 caaaccgcct tgcaggcaaa gaaactgaca gcatccgatg ttgttgtagg catcgccgcc
     9541 tgcggtctga ctccctatgt acacggagca ctgcagtatg caaacaaaat cggagccggc
     9601 actgtgttta ttacctgtgc tcccgaggcc gtcaggcaca tacccgcttc catcattatc
     9661 aatcctgttg ttggaccgga agtcattgcc ggttccaccc gaatgaaggc tggtactgca
     9721 accaaacttg tgctgaatac gctgacgaca gcagcaatga tcaaactggg caaagtgtac
     9781 ggcaatctaa tggtcgattt aactgcggtt aacgacaagc ttcgcgaccg ttcgatacgc
     9841 attgtgatgc agctgacagg gctgtcccgg caaaaagcgg ccaaattgct cacctgcgcc
     9901 ggcggcaagg tcaaaccggc tgtcgtcatg cattttcaca aggttgattt tcagattgcc
     9961 tgcaagctgc tgcgaaacca taacaattct ctgcgaaaca ccatccggga attcgtatga
    10021 cctgtctgca tcggattgcc tgcggaacga tcggctggat agatatatcc atcactgccg
    10081 ctgccatagc aatgcttttt gcaatttctt atgtttttgg gcgggatgaa aacgatacac
    10141 gggatttttt tctgggttct cgcaaaatcc cgccgtgggc ggcctgccta tcgttcgttg
    10201 ccacagaagt cagcgcgttg accattgtcg gcgtgccggc tacggcttac agtgaaaact
    10261 ggcagtatct tcaattcttt attggttccg ccgcagcccg gctggcagtt tgttttctgt
    10321 tcattccggt tttctaccaa taccaatgca ccagcattta tgaatatctt cggcaccgct
    10381 tcggggccca aacccaatat gccggttcgg tattcttctt tattacccgc ctgattgcct
    10441 ccggcgttcg gctgtatgca gcttgcatgg gtgtgggaat tataatgggc tggacgctgg
    10501 aagcgtctgt tgttttattt acgattgtca gcattgtttt tattgccatc ggcggcatca
    10561 aagccgttgt gtgggcaggg gcgtatcaag ctgttgtctt ctttgccgcc ggaggagttt
    10621 tactggctta cctgctgtac aggattgaag ggccgtttgc agcggcgctg caaatggccg
    10681 ccgatgccgg acgcttgagc atatttgatt tcagttttga actgaatcgg ccaaccagtt
    10741 tctgggccgg cagcattaac gctttttttg tgggtttagc ggttttcggc accgatcagg
    10801 agatggttca gcggctgctt acagttgaaa accgccaaaa gagtcagcgt gcccttttta
    10861 tgaccctgtc agccgccctg ccgctgttgt gcctgtatct ggcaatcggt accggtcttt
    10921 atatctttta ccagcagcat ccggatattc ctgctccggc acaagccaaa gaagttttat
    10981 cgcattttat ccgcctgtct ctgccggctg gacttaaagg actccttctt gccgctatca
    11041 tattggccag cattgattcg ccccttagtt cactggctgc ttcatttgtg tccgattttt
    11101 atcgtccggt cttagccaaa aacgccagcg aaagacatta tctgactgta tcccgtacag
    11161 gagtcgtcct gttcggtctg attctgggga ttatcgcctt ggcatgcagg ccggtagaga
    11221 atatcctatg gtttgctttc caaatcttct ccattaccgg cggcgctata ctgggcgttt
    11281 ttcttctggg gacactgacc aaaattcaag cgaactattc caatattgcc gccatgatta
    11341 tcaccgctgc ttcgatgacg gtcctgctgc tgctgaagca ttttgaaaaa atcggccttg
    11401 cctggagctg gctgattgtt gttggaacag ttcaaacaat gctgctggcc tgtcttttca
    11461 gccgcttggg agcagtactc ttcactgccg gccgagctgg acgctgagtt tatccagcca
    11521 tcgcttgtag gcctgctgcc aagcctcctg ccgggagcaa tcgggctcta tatgaccaat
    11581 cttttggaga ccgcagaacg catcctcgaa cgtcttgtaa aagccggatc cgatgccggc
    11641 cccgcgggcg gcgccctgcg aaccatcggt gttatacagc tcgatagcgg cctgtgtaac
    11701 atccgcaaat gccttggcaa acagttcgct ttggaacata ttggcccggc ctgcgcgaat
    11761 aacccgtaca tccagcccca tctgcttcat aatttccagc ccgtattgga atgcaaacac
    11821 aatcccttcc tgtgccgcac gaaacagatg tgcctgtgta tgcacattga aattcagacc
    11881 atgtatagaa gcatcgatga tgcggttatc cagtgtccgc tcggcaccat ttccgtaagg
    11941 caggatgcac agcccctcac atcctaccgg caccgatgcc gccagcgcat tcatctgcgg
    12001 ataatcctgc ccccctgccg tatgatgctt cagccagctg ttgagaatgc cggtgccgtt
    12061 gatgcacagc aatgagccgc agcggggatt ttggtctgta tgattaacat ggacaaatgt
    12121 attaacgcgg gagcgcttgt cccagcaagg gcggtcggct acaccatata caaccccgct
    12181 ggtgccggca gtggcagcaa cctgaccggg atgcagaacc cccagcgaaa aggcattgtt
    12241 cggctggtcc ccagcccgat agcagacagg cgtgccggcc ttaagcccca gatatgcagc
    12301 tgcctgctgc gtcaattcac cctgaatgct aaaagtatca accaccggag gaataatgtc
    12361 caaatcaaag ccgaaggcat ccatcactgg ttcggcaagc tgccgatgaa caaaatccca
    12421 caaaataccc tccgacagac cggacttggt cgtcagaatg cggtcggtca ttttcatcgc
    12481 cagataatcg cccggcagca taattttgtc aatgcggcgg tagatatccg gttcattctc
    12541 ctttatccaa gccagtttgg ctgcggtaaa atttcccgga agattaagca tcagctgaag
    12601 gcacttgtcc ggaccgatat gttgggcggc tttctgccca tacgggactg cccggctgtc
    12661 gcaccagata atcgatggcc gcagcacctg tttggcttta tctacgcaga ccaacccatg
    12721 catctgatag gtaattccaa cagcttttat tgcagaagga tcagcgcctg tcttgtccag
    12781 aatcagccgc gttgccgccg ccaaattctc ccaccagtcc gatggattct gctcagccca
    12841 tcccggcctc agcgacagta tcggcatttc tttcggcgga tacgacaccg aagcagcagc
    12901 attgcccgta gaaagatcca gcagtgtggc ttttatggat gatgttcctg catcatagcc
    12961 cattaaatac atttcaatcc tcctgtagtt aacaattggg cataagaaag tcggcctcgg
    13021 cggacctgct gccgaggcca accatatttg acaagcaaac ctttcggatg tgcctacccg
    13081 cggaaataca gatatgcagc aataattgcc gccatcaccg cacaggaggc gataacatcc
    13141 cacttgttcc aagacagacg cgactgacgg cggtcttctt ccgtcagggt accgtaggtc
    13201 aatcccttga tcttgtcata agcaggttct tcggtcatat agctgaccag aaccattacc
    13261 ccgatgcaga ccaggaaaat cagcaggctg taatactgga agtacatgtt gttgacaatc
    13321 cagaaaaacg acccttccgc ataggaaaag cccttaatca tcttaacggg cgtatcgaca
    13381 gccaaccgga acagacccag aataaagccg acaatcagcg cccacaggca gcccttggcg
    13441 ttgagccgtt tgctgaaaac acccagaaag aataccacaa aaatcggcgg agccagatag
    13501 gactgaatac cctgcagata atcataaaga cctttgccgc ctttgataac cggaatccac
    13561 agcaggccaa taagcaccat cactgccgtc gctacacggc caacccagac tacatgcttc
    13621 tgggaggcct gcggacgcag ttttgaatag aagtcgatgg taaataaagc ggcagatgca
    13681 ttaaacgccc ccgccagcga gctcatcaga gctgcaagaa gaccagccac cacaatcccg
    13741 cgtatgccca tcggcagcac ttttgtcacc agcagcggga aagcctgctg ggcattgtca
    13801 cggatcagct ggccgtcggc atcaaaaaag acttgcttaa gagcaggatt cataccgctc
    13861 ttggccaagg caaaagcaat catgccgggg atgataaaaa taaacaccgg cagcagcttc
    13921 agagccgctg cacatatcga gccgcggcgg gcaatctgct cattctgagc ccccagtaca
    13981 cgctgcacaa tatactggtc tgtgcaccaa taccacagac cgataatcgg ggcacaaaac
    14041 agcattccta cccacggata gttgtcatta aaataccacg ccatccgcgt tgattcttta
    14101 accggcgccc aggtagcact gacaccctgc ggtaccatcg gcttccataa gttaaacatt
    14161 tccgagccgg cccattcgcg caagacgctc cagccgccca gagctttaag tccgaaaaac
    14221 gttacgcagg ccgaaccaat caccagcaca attgtttgaa tcgcggaggt ataggccacc
    14281 gcacgcatgc cgcccagaat ggtataaata cccgtcagca gaatcaccag aatcgagccg
    14341 atccagaagc tgtcaagccc cataaagttg atatccggca tcatcacagc aaaaaccacc
    14401 ccgcctgcaa aaatgcctac tgccagtttc gtcagcacat aggccaccag cgagataacc
    14461 gacaaaaccg cacggttaat cggtgaaaac cgcttttcaa gaaactccgg catcgtaaaa
    14521 acctgtgacc gcatatagaa cggcaccaat acccacgcca aaaccagcag gcaccacgca
    14581 tgcagctcat agtgcgccat cgccacgcca tcggtggctc cggagccggc caaaccaacc
    14641 aaatgctccg agccgatatt cgatgaaaac actgctgccc cgatgaccca ccagccgaga
    14701 ttgcggccag ccagaaaata ctcggttgtg gtctgggatt ttttctcctt cagataggcc
    14761 caccatgcaa tcccaaatac aatggcaaaa taccccagaa ttaccagcca atcaactcca
    14821 tacaacaccg tcatattcaa tcctttcaat aaaatgtgac gttttgttta atctcagcag
    14881 atatagtcgt tgagcatatt ttccagaagt tcttggcggc cgctgaccaa ttcgggttca
    14941 ccattatcca agatatagcg ttccagttcc tcgaaaccga ccttgccttt ttctatcttt
    15001 tggcccagtc cggtatccca gccggcatac cgctgcttga gggcggcctc aaattttccg
    15061 tcatccagca ttttagcggc aatcttcaaa ccccgagcaa aggcatccat ggccccgata
    15121 tgcgcataga acaaatctgc cgcatcaatc gattgacggc ggactttggc gtcaaagttt
    15181 agaccgcccg ttgtaaaacc gccggccttg agaataatat acatcgccag agttgtatcg
    15241 taaatatctg tcgggaactg gtcggtatcc cagcccagca gcagatcgcc gcgattggca
    15301 tctaccgatc ccagcaggcc attagccgcc gccagcgcca gctcatgctg gaaggtatgc
    15361 ccagccaagg tagcatgatt ggcctcgata ttcattttga aatgctcaat caggccgtat
    15421 ttgcgcagaa aggccatgca gttgcctaca tcataatcat actggtgctt ggtaggttct
    15481 ttgggcttgg gttcaatgag aagctggccc ttaaagccga ttttcttctt gtgatcgacg
    15541 gccatctgca gcagcatcgc catatggtcc agttcgcgtc cgatatctgt attgagaagc
    15601 gtgtcataac cctcgcggcc accccagaat acatacccct gtccaccgag ttcgtgggtg
    15661 atttccatcg cctttttaat ctgactggcg gcataagcaa aaacatgcgg cgagggattt
    15721 gtgccggccc cggccatgaa tcgccggtgc gagaaggcat tggtcgtacc ccacagtaat
    15781 ttgacgccgg tatccttctg gagctgcttg gccttggcaa ctattttatc cagccggcga
    15841 tttgtttcgg ccaaattgtc ggcctctggg gcgatatcgc ggtcatggaa acaccagaaa
    15901 tcaacgccca gcttggtgaa aaactcgaag gcggcatcca ttgtctgctc agcggcctgc
    15961 atcggatcgg ctgcgctgtt gtaatttctg ataatcgtcg ggccgccaaa cggatctgaa
    16021 cctaaacctt taaaagtatg ccaatagcag accgcaaaac ggagatgctc ggccattgtt
    16081 ttaccccgaa tgatcgcctt aggatcataa tgtctgaatg ccagcggatt ggtcgattgg
    16141 gggccttcat acttaatctt tcctattccc gggaaatact ctttcatcgg tacctcactt
    16201 tctgcttgtc tacaagttcc aattgtctga cccagatatt cttaccgcag actgccggcc
    16261 agcatatagt atacttcatt ggtccgcatc tgacgcttga aatctggaat ctgcgtattt
    16321 tcgtcaatca gcagatactc gatgcccgcc atctccgtga agtcttccat atgttctgcc
    16381 gtcagggcag tcgaaaaacc ggtatggtgt gcgccgccgg ccagtatcca agccgctgcg
    16441 gccgttttga gattgggacg gggcacccag accactcttg ccaccggcag attgggcaaa
    16501 tccgcgtcag gcttaaccac atcaacggtg ttgacaatca tccggaagcg gttgcccata
    16561 tcaatcagag acgcattcaa gccggccccc ggtgcggcag tgaagacaac ccgaaccgga
    16621 tcggctttgc cgccgatacc cagcggatgc acttccagcg acggcttgcc ggaggcaatt
    16681 gtcgagcaaa cttccagcat atgggcgccc agcaccttca ttcccttcgg atccagatga
    16741 taggtatagt cttccatgaa cgatgtgccg cctttgaggc cggcagccat caccttcatc
    16801 gcccgcacca gtgcagcggt tttccagtcg ccttcagccc cgaagccata tccttcggcc
    16861 atcagacgct gaacggccag accgggcagc tgcttgagac catgcagatc ttcaaacgtg
    16921 gttgtaaagg ccttgaagcc gcctttttcg agaaaagcac gcaagcctgc ctcaatgcgg
    16981 gccgcttcac gaaccgactc catctgctcg gcggctacac ggaacttgta gcagtccttg
    17041 tattcggcaa caagcttgtc tatttgcgaa tcggcaactt tattgaccac cgccaccaaa
    17101 tccccgacgc cgtagccgtt gactgaaatc ccgaactgct tctgggcctc gactttgtcg
    17161 ccttcggtta ccgccacttc acgcatattg tcgccaaacc gtgcaacctt gagcgagcgc
    17221 atctcgttcc agccagcggc tgcccgcatc catatgccca gcttctgctg aacatccgct
    17281 tccttccagt ggccgacaat aaccttgcgg tttttgcgaa ggcgcgtgca gataaaaccg
    17341 aattcccgat cgccgtgggc cgactggttg agattcataa agtccatatc aattttgtcc
    17401 cacggcaggt cgcggttaaa ctgcgtatgc agatgcacaa acggcttctg cagtgtactc
    17461 aatcctgcaa tccacatctt ggcagggctg aatgtatgca tccaggcaat caaaccaatg
    17521 cagttggggg tggtattggc cttgacgcag attttggtga tttcgtcggg tgtcttaaga
    17581 acgggcttaa aaacgacctt gcagggaatc gctgcagaag catccagcgc ttcggcgatc
    17641 tgcttgctgt cagcatccac ctgtttaagc gtttcggggc catacagatg ctggctgcct
    17701 gtaataaacc atacttcata ctcttttaag tcaatcatac tatttacctc ctgaaaaatt
    17761 atttctgtcc ataataagcg tttttgccat gtttccgcag ataatgcttg tccagcagga
    17821 aatcagcaac agggattaat gagggattaa gaacagctgt atggagcgcc atgtgggcaa
    17881 cttgttcgag cacgaccgcc gactcgactg ccttggcagg cgttttgccc cacgtgaacg
    17941 gtccatggtt tgccaccagc acggcaggca tctgttccgg atcgagcttc tggaaggttt
    18001 taacaatcac acttccagta ttcttttcat aatcgccttc cacttcctga cgggtcatcg
    18061 gcttggtgac cggcacaggt ccatagaaat tgtcggcgtg ggttgtcccc aaacagggaa
    18121 tcggccggca ggcctgcgcc caaaccgttg cataggtgga atgcgtatgg cagatgccgc
    18181 caatggcgct gaaatggcgg tacagttcca gatgggtcgg cgtatcggaa gacggacgca
    18241 gctgaccttc tacaattttt ccgtccaaat ccagcagaac aattttgtcc ggcgtcaatt
    18301 cgtcatagga caccccgctg ggcttgatag cgataatacc ttgctgacgg tcaatgccgc
    18361 tgacattgcc ccagctgtaa atcaccagag catgcttctt cagctccaag ttggcagcac
    18421 agacatcaca tttgagctgt tcgagcatag actactcctg actgctttcc ttaatagcca
    18481 gcagttcctt catcacattg gccattccga tagaattgcc ggacaagccg aaaccatcgt
    18541 gaagctgctt atagagtttg tacagctttt gatagacctg atggttcttg ggattgggct
    18601 tgaaggtctt ggctttgatg ccgcacatcg ccttctgggc ggaatggtaa tcggcaaacg
    18661 ccccgcctgc gaccgcccct gcgatagctg aacccaatgc acaggtctgg gcggaccgtg
    18721 aaaccttcat ctcgcggccg gtaacatctg cgtaaatctg catcagcagg ggattttttt
    18781 cgctaatgcc gccgcagttg acaatctgtt caatcttgac gccgtattct tcgaagcggt
    18841 tgatgattgt cagtgcacca aaagcagtcg cctcaatcag cgcccggtaa atctcctcgg
    18901 gcctggtatg cagcgtttgg cccaacagca aacccgtcag ccgctggtcc accagtatcg
    18961 tgcgattgcc gttattccag tccagtgcta aaagacccga ctgtcccgga cggagcttgg
    19021 ctgccttctt ggtcagagca tcatgactgc ccagcgtctt gccgcccggc tggatatagt
    19081 tgacaaacca gttgaagata tcccccacag ccgactgccc tgcttccagc ccgtaaaatc
    19141 ccggaagaat cgaaccgtcc acaatcccgc agatgccagg aatatccggc agtgtggcat
    19201 cattgggggc aattgccata tcacaagtac tggtaccgat gattttaacc agcgttccgg
    19261 gctgaatgcc cgatccaaca ccacccaaat gggcatcaaa agcccccatc gccacaggaa
    19321 tcccgggctt taagcccagt cttttagccc attggggcgt caaatccccg gctttgtctg
    19381 atattgtata ggtcttgttc ggcaaggtac gccgcaaggc tgccattttg ggatgcagct
    19441 tggccagaaa ctcctcggaa ggataaccgc cccaggcatc attaaacatg gccttgtgtc
    19501 cggccgcaca gcggcatcgc ttcatcctgc tcggatggtc cgtacccgtc aacactgctg
    19561 taatccaatc cgcatgctcc acccatgtgt aagccgcatc gaagactttc ggatctgtcc
    19621 gcaggcaatg cagaattttg ctgaaaaacc attccgatga atagataccg ccgcatttgg
    19681 ccagatattc cggatgctct ttcgctgcaa gctgggtaat ttcagcagct tctgcataac
    19741 ctgtatggtc tttccacagc cagacacagg cattcaggtt tttcttaaac tcctttttaa
    19801 acgccagcgg cacaccttcg gcatctaccg gcagcggcgt cgagcctgtg gtatcaaccc
    19861 cgatacccac taccgcttcc ggcgtaaacc gcttgtcttt ggttttggcc tgccggacag
    19921 ctttttttac tgttacttcg atgcctttga ggtaatcggc cgggttctgg cgggcaaggt
    19981 tatggtcaga cggatcaagc agaatgcctg cctgccccga ttcgtaattg tagatgctgg
    20041 tgccgacctc tttgccgttc tgggtgttaa cgatgagaca gcgaactgaa ttcgtgccgt
    20101 aatcaagacc gattgcgtat gccataactc tactccctta taaagaacct ctcccatact
    20161 attgctgtaa ttgatgcgga attgtaataa aagcggctgt tgacggcaag tccaatttgc
    20221 gttaaaatat caccagtttg cgcataatca aaatcgtaag ggtgccgctg atgcataaag
    20281 tcattattat ggttgatact gcccgtgcct ccggccgcaa atttcttcgc ggcatcgagc
    20341 ggtacatcag ctctaacgct gactgggagg tatgcataca gccgcctcac tacctgcccg
    20401 gcacccactt caatccaacc tcatggttca aactgcagga agccgatgga cttatcgacc
    20461 gcgattccaa ctgcgccgat gcatttgttc atcttcaaat ccccaagatt atcagcgata
    20521 ccaaactgga aaatcccggt gtatccacca tctacaccaa ttccgaaaag accggccgaa
    20581 tcgcggccga atattttctg gaactgggtt ttaagcattt tgcatattgc ggctttgaag
    20641 gtctggcctg gtcggaaaga cgatttcgca gttttgcggg ggttttgaaa aaaaacggtt
    20701 tcagctgtgt ttatcattat catgattggc tggccaatgc cggcagcagt gaaaccgaag
    20761 acagccggct ggctcaatgg ctaacaaccc tgcccaaacc cgtctgcgtt ttcgcctgca
    20821 atgacgatcg cggggtgaat gttctggagg cctgcaagat cgccggtctg acggtgcccg
    20881 aggaggtagc ggttttgggc gtggacaacg acgacctgct gtgtgacttg tcatctcccc
    20941 cgctgtccag catcgagctg aacttcgaac gcggaggatt tgaggccgca aaacttctcg
    21001 atcaaatgat gacccgcggc atggcgcata ccaatattgt cattgaacca gtagatatcg
    21061 taacgcgtca atcgaccacc atcttggcga ttgatgaccc ggaagtagtc aaagcactgg
    21121 tatttatccg cgaacactac gaagaaccaa ttcaggtcaa agatgttgta gcagcaactg
    21181 ttatatcccg ccgggatttg gaaatgaaat tcaaaaataa gcttcggcgg tcaattaaag
    21241 aggaaataga ccgtcttcgt ataaaatccg tacagcgtaa attggccaac accagcgata
    21301 cgattcacaa tatcgctgct tccttgtcat acaccgaccc gcagcacttt tcgcgattct
    21361 tccgaaaagc cgcgggaatc agccccagtg cctaccgccg cctcaataaa ccttacatgt
    21421 aggaaagggg tagattttct tggcctacga tccctaaaaa ctgtaaaatg gctttccagt
    21481 ttttagaaca atactttaca ggagaatgga ttatgaatcg cagagcattc ttaaagacca
    21541 tgccggcgat agccgcattg accgcagcgg atttagattt tgctcaagag gccgacttac
    21601 cctctgccgt ttcacaaact tcgtcatcca tcggtaaact gacacctatc gaacttgtca
    21661 aaccgtctgc tgacgggggt aaatccgtac tggcggcgtt atgggaacga aagaccaatc
    21721 gcagcatcag cgaccgtccg ctttctgacc aagatttgtc caatctcctc tgggcggcat
    21781 tcggagcaaa ccgacctgac ggtgtgcgcg gcaaaccggg ccgtacagcc gccacggcca
    21841 gcaacgcgca ggaaattgac atctatgcgg ttttgccggc agggatttac cgatacgatg
    21901 ccgtggcaca cagactgatg ccggcagtcg agggcgacca tcgcggtctt gtcggccgcc
    21961 gccgccaagg cgcaatggca gcagcaccgg tgatacttgt ctatgtcgcc gatatcggca
    22021 aatacaaatc ggcccccttc caagaaccgg ggcttcggga tcctgaaata cagaaatcgt
    22081 attataacgt tgccatcggg ctgattgcag gcaatgtata tctctttgcc gcttcgcagg
    22141 gattggctgc ctggtttcac aattgcaata agccggcact ggcagaggtg ctgaagctgg
    22201 ggcctgagca gtatgtaatg tatgcccaga ccgtgggaca tccagcataa acagcataca
    22261 atcagaaaaa cctgtatttt gccgcaatcg gccaaaaaaa agattatgcc gttatttttt
    22321 caatctattt gcagcagcag ttcaggcaat tgccttactg tctggtctaa cacaacgcgg
    22381 cttgtgattg actgcggatt aacctcaatg cggcaaacca gacaggtatt aacggctttg
    22441 tcggcacaat ctaaaatcga ctctgtgctt ttgacacaca cccacagcga ccatatctcc
    22501 tactatcctc tgcgggtact cgaagaactg gggcttgcaa ttcatctgca cagcgattgt
    22561 gcagaccagc ttcgtgatat ccatttcaac gattacggct tcccaaagct gaaactggta
    22621 ccctttcaga ggaaaaagtt tgttgtagga gatttctgca ttcaaccctt tgaggtggtg
    22681 cataatccgg atttccccac cttcggctac cagatagatt atcagggcaa aaagattctc
    22741 attgttaccg atttcgcccg gtgggaagac ctaatcgatt attttttgga tgcggatttt
    22801 attttcgttg aatccaatca cgacctcggc ctgctggcat tgtatttcaa tcccaacagc
    22861 cggtttcata tgtccaatcc cgatacggcc aaactgctgg tgaatgcccg caggggaagc
    22921 agaaaagccc ccgtatcagt catgctggga catctgagca gtcagagaaa caatgcccat
    22981 ttagccttgc aggaaatcca aaatgccttt cggaatgctg atattgaact ggattttcat
    23041 cttcaaaccg ccccattgaa ggaagcttca caggccatcc ttctcatgtg acggttgtcg
    23101 gcaccctatt ttacagccgt tttttacggc gcacggcatc tctcaaaaca ccgtcggcct
    23161 ctctatatca gcagccaaac ggatttctac tgccgagcca atctgcataa gatctcaaca
    23221 cctttgtcga ttttgtcctc ggttgtggta taagcgatac ggaaatgcgt atcctgttca
    23281 ctgaacacat tgccgggaat aaccagcagg ttgttgtcaa tcgctttttt cacaaattcg
    23341 gttgcattag gatatccagc cggggttttg gcaaacatat aaaaagcccc cgcgggcaaa
    23401 accacctcaa atgcctcctt aagacccgca tagatacggt ttcgtttgcg gcgatagatg
    23461 tcgatatagt cagaaatatc cagatccatt gccgccacgg ctgccatctg aaacggcgtc
    23521 ggggcacaga caaaggtgta ttgctgaatc tgaatcatct tgtcaatcag cggtttgatt
    23581 ttttcacttc cgacggcata tcccatccgc cagcccgtca ttgcatagct tttgctgtat
    23641 cctttcaaaa ccagcgtgcc gtcataataa ttgcccatgc tgatgcagcg gccgtcataa
    23701 cagaaatcct cataaatttc atcactgata accagcaaat catgccggcg ggcaatatca
    23761 gcaatcgccc gggcttcctg ctcggtatag accgcccctg tcggatttgt cggagaattg
    23821 agcacaagca tcttggttct gggcgtaatg agcgattcaa gcctgtcgat aggtagcttg
    23881 aaatcaggat aacagtcggc aaacacacat gtaccgccca gcatccgcac cagatgcttg
    23941 tacatcacaa aatacgggtc gggaatgatc acctcatcgc ccggattaac ggtggccata
    24001 aaggacagca gcaggcctcc gctgacaccg ctggtcacca tcagtggagg attatcccac
    24061 ccataatcaa ccttgacttt gtccctaatt ctctgcagaa gcttttgttc acctgctgtt
    24121 tgggaataat ggttgaatcc attttcaatt gcttttatcg cggctttttt aaccggctgc
    24181 ggcacatcga aatccggctg accaatagag aaattgaccg gatctttcaa ttctgccgcc
    24241 aaggcaaaga cctttcgaat accgctggag tcaatcaatt gtgtccgatc cgcaaggtaa
    24301 ttcatttctg ctctcccacg accgctattg tttcggcgca tagcaattgc cgcttgcggt
    24361 tttggaatat ccctgcaaag cggcaattct atatcggtac aatccctatg gcttccgatg
    24421 ggctaccttg ggcagtccca tcactttatc gccttcctgc cagcgttcct gctgcttgag
    24481 catctgaatg cgctcttcac gactcagaac attgcgatgc cgtttcaagg cgcctttgat
    24541 tttcaacgaa cgatctacag acattttgca tcttctcctg tttacctgcg acaataatcc
    24601 gattatctct ttaatccgta cgcatcctga aacggcttga gaccaaaatt ggtgtctccg
    24661 gtttcagccg gatacagcaa gccgagctgc tggatgcgtt ttttcatttc gtacccatcc
    24721 gttccctcta tggcagggtc ctgcataaac aactgtttgg taaccagcaa accatatccg
    24781 cgattttcaa taatctctgt ttcgataccc ttgcttttga agtaatcttg tatcggcttc
    24841 agctcattct tccgattgag ggatataccc tgtatcacga taacatttgt ccccttttca
    24901 ggtttcaacc gcggtaattc ttcagacaca atcggtaccg gcttgggaac ttctgtttct
    24961 tttcgaacag gcaccagcgg ctccttaaca ggttctggag tcgccgccgc tgttttttgc
    25021 tcctgcctta ttatgacaga tgggctttcc tgtttaggtt caaggccgga ttgaaccgca
    25081 cctttttgcg aatatattgt accgagccaa aaaccaacag ccaaaacaag aatctgcaca
    25141 accgctgcca ttccaatcag aagcactttg gctgaaacag caaaattagg tttatctgag
    25201 acttcttctt tatatacccg accagattcg tccctaattc cggaaaattc cgagtttcca
    25261 gacgctggtt ttgctttttt taacaattct ttataagggc catcagaccg catttgtcct
    25321 gttttcagcc cttttgcgat ttttgcctgt ccttggcgta tggcctcgct caatgccttt
    25381 tgatttaaac ttcggcctgt catcctaaaa acctcgacag atgtaatgcg gtccctgtcc
    25441 caattaaaaa aacagggtat attgttttct tacaaaaaat aaagatatat tttattaccc
    25501 tatcttgcag caaaatgcaa tattaaagtt gcggtaaaaa tgataatttt tgcctggcag
    25561 cataaataaa gacttgactt gtcaggcttt aaacggtata ggggttggtt ttccataaga
    25621 attttcttta cgatgcttca tcattttgat atagattttc agtcggatag agatacgaat
    25681 atagcgccga caaagatacg ggagtgtgca tcttttgtat ctataaatca cgaagacata
    25741 aaaagtctat ctatcgtaaa cggaactttt tggagataaa agagttatgt taccggtaaa
    25801 aaaagtcagt aaatcacgga cacggacacg ccgtagccat cagcgtcttc gtccggctaa
    25861 ttattcttta tgcaagcagt gcggcgctgc gaaattgcct catacggcct gcagccagtg
    25921 cggatatgtc aatgagaaaa ttaccttaaa gcttgccaag gagtctgaca gctgatatgc
    25981 gtattgccat tgatgctatg ggcggcgatt atgctcctgc tgaaatcgtg gccggagcac
    26041 tggaggccaa ggaagtcctc gaccacagcg atgagcttgt gctggtggga aatgaagatg
    26101 tcattcgaga gcacctgaag cataatggag ccagtcctga cacctttcga attttccatg
    26161 cacccgagat tatcggaatg gatgagtctc ccgtagaagc gcttcggcgc aagccgaaaa
    26221 gctcaatcgc cgtgatggcg catgctgctt cccatcgtca ggtggacgca gtgctttctg
    26281 cgggcaatac aggggcctgc gtggcagcca gtcagcttcg gatgcgcaat ctgccgggag
    26341 tgctgcggcc gggcattgcc gttgtgctgc cgacattagg cggcccggtt acaatgtgcg
    26401 atgtgggcgc caacattgtc tgtaagccga ttcatctcta tcaatatgcc gtgatgagct
    26461 gtgtgtacgc agaagtgatg ttcggcatcc agaagcccaa ggtcgggctg atgaacatcg
    26521 gcgaagaaga ggccaaagga accgaccttg tcaaaaaaac ccgtgatctg ctccgctcgg
    26581 attccaccat caattttgtc ggaaatctgg aaggacgcga tctgttcggc ggcaaatgta
    26641 atgtggttat ttgcgagggc tttctgggca atgtgatact caaattgacc gaaggccttg
    26701 tggatatgat attcagtgct attaaacagg aactcaaaca ccgcaatccg ctgatgacgc
    26761 tgtggtttaa gtctgtgatg aaaggactgt tccgaaaata cgactaccac gaatacgggg
    26821 gggctttgct tctgggagtc aacggcattt cgattatctg tcacggttcc agcaaggccc
    26881 gaaccatcaa aaatgctatt cttgcctgta agaaattctc gacactgcag attaacgaaa
    26941 aaatagtaga acgcttaact caatccacgg tgacacccaa tggtcaataa tccaacggtt
    27001 caaacaccca aacgccgggc gatactggcc ggcacaggat cgtttgtacc ttcaaaaata
    27061 ctaacaaaca gcgacctgac caaaattgtc gatacttccg atgaatggat aaccacgcga
    27121 acgggcatta aaacccgtca tgtggtttcg gaaggcgaaa cgacagcaac gctggccgcc
    27181 gaagcagcac gccgggcact gaacgacgcc ggcatgcggg cggatgaact aaacctgatt
    27241 attatcggaa ccattacgcc ggagatggtt tttccatcta cggcctgttt tgtccaagat
    27301 atgattggtg caaaaaatgc atgggcgttt gatatttcgg cggcatgcag cggttttgtg
    27361 tatggtcttt cgattgccca gcagttcgtc agttcggggc tttatcagaa tgcactggtg
    27421 attggggcag aaaccctgac gcgaatcaca aattatcagg atcgcggcag ctgtatcctc
    27481 ttcggcgacg gtgccggggc tgtgattctc aaggccgcca attccggtcc gcagggagtg
    27541 atgtacagca catcgttttc agacggaagc ggatggacct cccttaactg ccctgcgtac
    27601 ggctcgcggc accctgtcgg caaacccctg ccggatccgt cgatggttta tatgcacatt
    27661 aacggacgcg aggtttatca aacagccgtc cgccgaatag tagaactggt taatgaatgc
    27721 ctcgaaaagt gtgatttgga tgtggaagat attgcaatgt tcattccgca tcaaatgaac
    27781 gcccgcatca ttgaatcggt cgggaagcgg ctgaatttct ccgatgaccg cattttcatc
    27841 aacatagaca aatacggcaa cacttctgct gcaacgattc ccattgcacg tgacgaatgc
    27901 cgccggaccg gcaagcgtaa atcgggcgat attgtgctgc tggtagcctt cgggggcggg
    27961 ctgacctggg gcgccaatgt tattcagctg tagcagaatg ttatttggga aggaaaaaca
    28021 gggacaatga aaacagcttt tttatttccc gggcagggag cccaatatgt cggcatgggc
    28081 cgggatattt atgaacaata cccgcaggca caagcccttt ttgaacgagc cggcactatt
    28141 ctggggtacg atttggcaga tatatgtttt aacggccccg acgacaaact caacgcaacg
    28201 gaggtatcgc agccggctat tttcaccgta tcggccgctt ttctggagct gatgaaggca
    28261 tccccaaagg ctgttcaccc ggatgctgcc gccggattga gcatggggga atacactgct
    28321 gtttacgcag ccggattgat ttcgtttgaa gatggcttgc gtctggtcca gaagcggggg
    28381 caggctatgc aggcggccgc agaagcaaat ccgggagcca tggtcagcat tctgggactg
    28441 gatgatgtgc agattatttc gctctgtgaa gaagcttcac agggcgaact gcttgtcgca
    28501 gtcaacttca actgtcccgg gcagatcgtc ctgagcggaa ctctgggggc atgccagcga
    28561 gccgctgaac tggcggctaa atacggtgcg ataaaggccg tgccgctgag tgtggccggc
    28621 gcattccatt cggaaatgat ggctccggcc gcaagccagc tgggccaagc gctggccggc
    28681 tgcccaatcc gcaatcctgc tgaaatcaag attatcgcca atattcatgc ggactactat
    28741 atatcggctg acgatatccg gcagggtctg gtcaaacagc tggttcagcc gatattgtgg
    28801 caaaagtgca ttgaaaaact gctggccgac agctttagca gtttctatga aatcggaccg
    28861 ggtcgggtgc tgacaggtct gatgaaacgc atcagccgac gcagtcagat taccaatatc
    28921 agtacggcag aatcgcttca ggaatttatc ggttcaaacg cataagaaat atcaggaata
    28981 gacaggaagg actaacagat gtcagaacag cgtttagcag tcgttaccgg cgccgcccgc
    29041 ggaatcgggc gagcgattgt tttagaatta ctgaaacagg gacggaaagt cgccggtctg
    29101 gatttgaatg cccagcagct tgccgaactg gaaaaagtgg tttcccagtc cggttactct
    29161 gtcatcacgc gagaaattga tattacccaa accgaccagt tgacccagat tcttgaacag
    29221 cttgccgaag agcatggcgg catcggcatt ctggttaaca atgccggcat tacgcgcgac
    29281 cgactcatga ttcaaatgga cgatgcagat tttgacaagg ttatcgctgt taatctgcgt
    29341 gccgcgttcg ttgctacccg tgttgctgcc cgctcaatgg ttcgcaacaa attcggccgg
    29401 ctcattcata tcagctccgt agccggcgtt atgggacagg ctggttctgc gaattacgct
    29461 gccagcaaag cagctttgat cgggatgtcc aaatcagttg ctcgggaact gggcaagaaa
    29521 aatgtgacct ccaactgcat agcgccgggc tttatcatga ccgatatgac gcaggtgctg
    29581 cccgaagcgg ttaaagaagc cgcaatggct gttatcccaa tgagacgctt cgggaagccc
    29641 gaagaagtcg cccgtgccgt ggccttcctt gcttcggatg aagccggcta tatcaccggg
    29701 caggttttat gcgtggacgg cggcatggcc atgtaagaat tttgccaaaa aaaggttgaa
    29761 aagccgttgt tcggccggta tattaccgcc gaattaaaaa tgtacataaa aggactgaaa
    29821 ttccgtaact gttatgattt aaggagatag aactgtggca gacgtagata tcaaggccat
    29881 tgaagagaag gtcatcgaaa ttgtaagtga acagatgggc gtcgataagg gagaaattac
    29941 acgggaaaca tcctttatca atgacctgaa tgccgattcg ctggatacag tggaactggt
    30001 catggaattc gaagacgaat ttgatatgag catcccggat gaagaagccg aaaaaatcca
    30061 gactgtcggc gcggcaatcg actacattgt agaaattatg aagaacaaag aagcataagg
    30121 gcaatcatga ctgggcgtcg cgtcgtcatt acgggattgg ggtgtataac accgctggct
    30181 gaatccgccg aagcgctgtt tgacgggctt tgtgaaggca gaagcggcat ttcgatgatt
    30241 gagtcgttcg atacttcgga gtatccggtc cggttcggcg gagaagtcaa gaatttcgat
    30301 atagaacggt acaccacagc ccgggacgcc aaacggatgg accgctttac acagatggct
    30361 cttgcttcgg ccattcaggc agtcagcgac agcggcctga acttcgaaca ggaaaacaag
    30421 catcgggtcg gtgtgattgt tggaaccggc atcggcggga ttaaggagat tgaagaccag
    30481 cacatccgtc ttctgtcgaa ggggccgcga aaggtatcac ccttctgcgt tcccaagctg
    30541 atgggcaatg cggccagcgg atgcatttcc atcgaatacg gcctcaaagg gccgaatatc
    30601 tgtgtggtaa ccgcctgtgc ctcggccagt catgccattg gcgaagcctt ttggaacatc
    30661 ttgtgcgatc gttccgatat ctgcattacc ggcggttccg aggctgcctt gacgacgatt
    30721 ggtctggctt ctttttgtgc cctcaaagcc ctcagcaccc gcaacaacga tccgcagcgg
    30781 gcatcgcgtc cattcgacaa agatcgcaat gggtttgttt tgtccgaagg cgccgggatt
    30841 ctcgttttgg aagaatacga gcacgccaga aaacgcggcg caaaaatcta cgccgaaatg
    30901 ctcggatacg gcgctacagg agacggtcat catatcacag cgccgctgga agacggctca
    30961 ggagcagctg acgctatgcg gctggctttg cagcaggccc gcctgaatcc tgaccagatt
    31021 gattatatta atgcccacgg caccagcacc gaattaaacg atgcagccga aagcagagcc
    31081 attcgttccg tcttcggcag ccacgcctac aaattggccg tcagctcttc caaaagctgt
    31141 ctggggcatt cgcttggggc cagcggggca attgaactga tagcctgtgc caagtccatc
    31201 gaaaaacaga tcattcaccc cactgccaat ctggaagtcg tcggggacga ttgcgatccg
    31261 aaaatggatt ttgtgcccgg caaggcccgc cagacatcgg tacgctatgc cttgagcaat
    31321 tcgctgggct ttggcggcca taactgcagc ttaattatcg gcaaattgta agcggtctga
    31381 atcagctatt tccggcctcg cctttcctca aaggcggggc ttttttattt tccgtttgcc
    31441 ctaatcaggg ctgttctttc tgcatctgct ccagcaggcg ggcttcttcc tgccgcagtt
    31501 tgtcaaacaa atccggccgt tccagctgaa tgcggttaat taagctctga cgcatataat
    31561 ccggatacat tgggtcattc aaaaagccga tgaaggcctg ataccgcagg aaacccatcg
    31621 gcggaagccc cacccgtccc ggctctgcct gtcccatctg ctgctgataa atagaataaa
    31681 tctcctgcgc catattttcc cgccctgcgg cctcatcatc atcgtggagg gcgtagcgga
    31741 aatatccctc ccgcagcacc gacaaaatga actcggtagc atcgttgatg ctgatgcctt
    31801 ccagctcgct tttgagtcgg atgcgtaaaa agttcatcaa ggagccggta tattccgtcc
    31861 tcgtatagcc ggattcgtcg atcaaatgct ctttctgaag ccgcctgtag atctgctcgg
    31921 ccttgcgctg atggccggcc tgataaaaca tcagaaccgc attttccaga aaattcttat
    31981 ggcctcccct gacggctttg ggattgcctt tttcaagcga ttcgtacttt tcaatgacct
    32041 tcttccacaa ttggtcgcag ctttcaaaca tgcgcagatc cggcagcaaa tagacagacg
    32101 gcggctgatt gggcacatcg taaaggacaa ctttgccggt ccggtacagc atctgcagac
    32161 tgtgaaagac gatgcggtct gtgtttttct cgtcgatgcg gtactcttcc gggcgtccag
    32221 ccaccttcag ccccagcgcc gcccaataga tagcatgggc agccggatgc tcccaattca
    32281 gcggcatccg ctcattggga tcatcaatac tggatggtcc gtaagttcgg ttcagttcca
    32341 tcatgaaatc gatatcaaac ttccactcgt tccgcagctg ccaagaccgc gcaaatgtat
    32401 caaatgttct gagtgcctct gtggaacggt agcggtcaat tacatcaaag gcctcttttt
    32461 tgaaacgtgc cggcgttcgc cgaagtgtca gataattggg tatcagcagt ttggcgtcag
    32521 caaacgcgct gtcggcctgc tgaagctcgg caatcaaacg agccgccgct ggatctgtga
    32581 gaacttcatc gagcgttttc ggtgcagaag ccagccgctg aaatgtctca ttggtcggat
    32641 tttcacccag caaagaacgc atcgacaaag ccagttcacg cttataatag cgatggcagt
    32701 catctgaaat gtcgccgatt ttatgctgaa agatccaggc cagagaccga tacagcagaa
    32761 tgctgcgcgg attcaggaca atggcacggt cccgcagcag ttcgtagccg ttctttaccc
    32821 agcgccagcg ctcctcgggc tgggtgtttg ggatggcaac tgaaatatta taggccatgt
    32881 tccacgcatg gaagtcccag acggcggcaa aacggggctg cagtgcggta atccattcgg
    32941 caagctgacg ggcatcgaaa aatttccctt cctgcttaag cgtatccgcc cgcatccaga
    33001 gaatatcgac aatcagaccc cgaaaagccc ccattgctac ggtcgcgaag gccagtgacg
    33061 gcggcgcatt ttccaaggat ggattagcga ccaagcccat tttttcgcgg gcctgctgca
    33121 gcggaaccaa gcgactggaa gcggcggcca aaaaaccgac tccgataacc gcacttgtta
    33181 tactaatcag ccacgctttt gtccgcatgt tgcaatcctt atacaaccgc cttagcgact
    33241 tcccgacgac tgaaaatcag gataccagcc agaaacatca gcaatccttt gatgcatact
    33301 gttatcccca acgtcgcagc taaaaacatc caccgcattg tccggccatc aacaatatag
    33361 cggttgggat tataagcacc gtcaaactgc ggcaacagcc acagcagagg tttaatagta
    33421 aatttataaa ccaccccggc cgcagcgggc agactctcta tggcatcaag aataaatcca
    33481 ttcgtcagac cggcaagaaa tacaatcagg caaatcagga tgacaaccgg aaatgacagc
    33541 catgtcgaaa gagacagccc caaaattgcc aaaaacatca gacggataaa aatcatcaga
    33601 accgcacgaa aataattttc cgtaaaagtg ccggttcggt acagcacttc cagttcttcc
    33661 ggaatcaccg tggtctggtt catcgccgga ttgttataga atgcaatccc cagaaatcca
    33721 tcctccgcca aagcattggc aggaacataa aattcatgaa ccgtgcggat gctttcggcc
    33781 tcttcatacc ggtaaacagg cgtgcgaaac tgaccgcctg aacggaactg ccggatatcg
    33841 cccacccgcc acattccaaa caccttttcg tccggcgggg ttaccgtaac ctgatattta
    33901 tagcggacaa acagcagtgt attcggatca ctggtatttt taatccggat attctggaac
    33961 tcccattctt tgacttcccc cggatccacc ttgcgggcct tcatgctctc ctgaccgatc
    34021 aattcgctca tgacccgcga ataaggcatt ccgtcgggaa tctggccggc ctctttaagc
    34081 tctttatacc gtgccagcgc ctgcttggcc agctggtctt tatccagctg ggctttgatg
    34141 cccagccgcg cggtaaaaaa ctccgcctcc gccttctctc tctgctcagc cggggctttg
    34201 gccgtttggt aaacaaacag tacgctgccg tacacaacac cactgaatag acccagcagc
    34261 agtgtgtcca ataaaagaat gcccagcagt tttccaagca tatattccac ccggcgaatg
    34321 ggttttgtta caatcagaaa aatatgcttg cgcttaagat cgctgctgag tgtgaaggca
    34381 gacaccgcaa tagtcagaat acacagcagc agactgatca ggcccaaacc gtagctggta
    34441 aaggtctgca gcttgcccag cagggtgcca tcgccttcca taactacgct catcagcggc
    34501 aacaggatac ccagcagcag aatgatcaca accgcgattt tcatgcgcag ggcctgggcc
    34561 agtgtatttt gtgctactgc ccagatgcta tgcatttttt gaatcctccg ccttaacagg
    34621 ctgggtcagc tgggtagaac catcccgctt tttgcctatc aattcctcca gaacatctct
    34681 tttgacggca tcttgtttct caggagcaga ggctggttgt acggtttcca cagccgtctg
    34741 gctcacaccg gcatccaccg gctgaaccaa tttgccaagc agatctctgt cagccgctgc
    34801 aggctgcggc tgtggtgccg gtttagtgac agctgcctgc ggtccttgcg gcgctgccgg
    34861 ggccgttaca ttagccgcca caagctgctg caaaacggat tcaggacgaa ctaccggttt
    34921 ttcagccaaa aatccctgaa taccggtgcc aacctcggca ccgctggtcc gctgcttctc
    34981 ctgacgggct ttggcaacaa tccgaataaa gaaattttcc agcttatcca tcggattcgt
    35041 aatttccgga acctgtcctt cacttgcggc tatccggcga atttgctcga ttgccgcctc
    35101 gctcaatctg ccggtgcgaa tctgtgtttc atcggactgc tggagcagct ggcggacagt
    35161 tccttcgacc tgcatccggc cgccgtaaag aatgccgatt cggtcacaga catcttcgac
    35221 atccgccagc agatgactgc acaggagaat tgtcttgccc cggcgggcca gttcaataaa
    35281 caggtccttc atctgccggg tccccatcgg gtccatgccg gaagtcggct catccagtat
    35341 cagcagatcc gggtcgttaa tcagtgcctg agccagcccg atgcgccgcg ccattccttt
    35401 tgaaaacgtc ccaatcgggc ggttgcccat tcccgacaat cccaccatct ccagcagtgt
    35461 ttcgatgcgg gctttgcgga tatccgcagg aaggccgaaa attctgccgt aaaaatccaa
    35521 agtttcgcgc gccgttaaat agcggtaaag ataggactct tccggcagat aaccgatacg
    35581 ggctgaaatt ttcgggtcgc tgaccttgcc gcccagtaca aaggccgctc cgcgggtcgg
    35641 atacagcagc tccagcagca ttttaattgt ggtggttttg ccggacccgt tggggcctaa
    35701 aaatccgtaa atctcattgt atcggatttt gaggttaagg tcttcgaccg ctatcacctt
    35761 cggccggccc caccagtccg gaaatatctt ggtcagggat atcgtttcaa tcgcaaattg
    35821 attgtctgct gccactgtca atgctccgcc ggagattaga cttgctgggt atggggctcc
    35881 aggtgctcac caaaccgcac cagacgggca ctgttgaaaa tgactgccaa ggccccgaac
    35941 aggtgcagga cagccgccat aatcagcgac acccagccca ctgtaatagc aataatgccg
    36001 aacacaataa acaaaatacc aaaaaagagg ttttgattga taaccgtccg cgttttgcgt
    36061 gataactgca ccaagaacgg cagacgccgc aaatcattgc tcatcaaggc aatagaggcc
    36121 gaattaatcg ccacatcact gcctgcggct cccatggcaa ttcccaaatc gccggccgcc
    36181 agagccggcg catcgttaat cccgtccccc accaccgcta ccaaatggcc ttcccggcga
    36241 atggcttcga caaccgccaa tttgtcctgc ggcagacagt gcgccttgaa atcggtgcat
    36301 ccaagctcgg ctgatacccg gttggccacc tcgctgcggt cgccggtcag catggtgaca
    36361 cggcgcacgc tgcattcctc aaagagctcc ttgaccgcct gctgtgcttc cggccgagct
    36421 ttgtcctgca gaccaatcca gccgacacac ttgccgtcag cagccatata gagcgtgctg
    36481 aagccctgtt cttcgtgcag cgacgggtct gacacattgc tgatatcgat gccgttttcc
    36541 ttcagaaagc tgtcgcgtcc aaccagcacc gtccgccctt ccacaacggc ccgcacaccc
    36601 ttgcccggcg cttcctcgaa tgtctgggct tgggggaatg aaacattggc ttcccgagcc
    36661 aaattgatca ccgcacgggc ggccggatgc ttgctcattt gttctgccga ggccgccagc
    36721 atcagcatct ggaccggttc gacatccggt gctggttcca gtttcgtcac atacagctga
    36781 ccagtggtca gggttccggt tttgtcgaac accacagccg taattttacc ggcgatttcc
    36841 agcagcgaga cattcttgat taaaatcccc aagcgggctg ccgcggacaa cgccgccacc
    36901 attgccgtcg gcgtcgccat aatcagggcg caggggcagg aaataaccag aataccgatg
    36961 gacttatcaa gggttttcgt aaaaaaataa acaatggccg caatcatcag aatagtaggt
    37021 atataccact taatataccg gtcaatgatc cgcataatcg gcagctgggt ctgctcggct
    37081 gcaataatca gctgctgaac cttgcctaat gtggtatccg aaccggcttt ggtgacaacg
    37141 atatccagca cccctgtcag gttgctggtt ccggcaaaga cctgcatgcc gacaacctta
    37201 tccaccggca gcgattcacc ggtaatggtc gcttcattaa ccgtgctcag tcccttttgg
    37261 acttcgccgt cagccggaat attatctccg ggacgaaccc gaacaatatc gccgggttta
    37321 agagcgctga catcaacctg ccgttccgtg ccgtcggcat ccaccagcat cgctgtcgtc
    37381 ggcgtcagcc ggatgagaga ttcaatcgaa gcccgtgccc ccagggcggt gcgcgtttcg
    37441 agcagttcgg caatcagcat aataaacgcc acaacgccgg cctcgacata ctcgccgttg
    37501 gcaaatgcgg caatcaccgc cagcgccacc agctcgtcca tatgcatatg cccctgcaga
    37561 atgcctctga cggcatgaat gatgatcggc aatcccagca gaacggcacc tatcattgca
    37621 atgatttcat tctgttcggt actgccgcca tagagaccgc ccagccacga gatttgggca
    37681 atcttgctgt tgagcaccag aataccgccg gccaacgtgc ctaaaaaggc cagactgacg
    37741 cccagctgcc ggcttttggt gctgccgtga tcgcagcagc aggaatccat ttcatgtgct
    37801 ttaagaccta aatgtgtatg taccatattg ctttctcaac tggttacaag ctgcatactg
    37861 tcctatttgg agctttcctg tttggctttt tccttcttga tattcggatc ccggttaatc
    37921 atcacgcgca gttcccgcga acggccttca gccgacggct ggattaaaat cttttcgtcc
    37981 gctccggcca gcacctgttc gacggcatcc tgataaatct tctgcagcac cagttcggga
    38041 tgttttcggt attccggcag aagctggcga aggtactcgg cgttggcctt ggcatcttcc
    38101 accacacgcg tgcggtaggc gcgggcttcg gagattttcg actgcacctg tcccgacagc
    38161 ttggctacat actcttcctg ctgctgcggc gacaaaccgg gctgggtgag cagttcgaga
    38221 atgcgttcgg catccggccc gcccgtgtcg gtcagtttct tttctttata agcgcgggca
    38281 tcgatgcggg cctgctcgct ctcctgacgg gccttgttgg aggcctgaaa tgcctcctcc
    38341 acctgccgcg gccagatgat tcggttggcc cggacatcct tgatgccgat gccgctctga
    38401 atctggtcca gcttgtcctg caggatgacc ttgacagaat cggcaatgcc ggtctggctt
    38461 ttaatggctt cgtcgatgct gtaatggacc agcgtccgca caatcgcctc cgaggccatg
    38521 ctttcaagca tgggttccag cgtttgcgcc gccgcctcca gcatgtcttc gccgggcctg
    38581 cggtcgcgga tatacacatt ttcaaaaaaa tgcttcggag aatcgatttt ataggtaatc
    38641 gtccactgcg aatgcacaat attgtaatcg gctccttcaa tcccctccac ccggtcattg
    38701 cgggtcaggc aatagccgtc tttgagcgga tccagcggtc caaataccgg cgttttgacc
    38761 ggccgcagct tctcttcttc cgtctcaaaa taccagaacg aattgaccgc cagcgactgc
    38821 tcccgtttga caggaatccg gataacctcg tgaatcggtt ccggaaatgc aaacttaatg
    38881 cccggcttga gaacggggtc ctccgccggt ccctgaatcc tgccgaagaa cagcagcagg
    38941 gcttcttcat cctgctggac ctgaaaaatg ccgctgcagg caaacaccgc cagcagcacg
    39001 atcataataa ccttcaggat agaaaaactg acatgcagcg cctgtgtaag cgatttggtg
    39061 gatgcgtcca gcggctcaga cggcagttcc cgctgcggca tttttgcctt gtcatgcaaa
    39121 tggtgatgat gctctgccat aacttagttc cctgtctgtg aagcggctgt ttggggctgc
    39181 aggtccggca ggcctttcag caggccaatg ggttctgttt cgacacccag aacgatcgtg
    39241 ctgcgctcct tgagaatctt tttaagggcc tcgatatccc gcaagaacat cgccagttcg
    39301 ggatcggctt ccagcatctt gtaataacgg gctgcctcag catcgccggc accgcgaatg
    39361 gctttggcct gtgcatcggc cactgccagc agctccttct gtttggcatc ggcatcgctt
    39421 ttaatgcgtt cggcctcggc atttccttcc gccagtatag cctctgtttt gcgcttccgg
    39481 tcggctttca tccgttcaaa cactttttcc gtgacatcct tgctgatcat cagccgcttg
    39541 atgccgacag cgctgacttc gatgccgtaa tcatgcatcg ctttctgaag aatctggccg
    39601 gcaatttcgc tttcaatctg ttcaaactga attttctccg ggtctgtgtt gacaaattcg
    39661 ctgaaataat gccgtccgat aactgaattc tgggcattgc gcagcagact tttgagattt
    39721 tcttcggcac ttttaacatc cttgacggaa atcaggaatt tccccgggtc tgctatccgc
    39781 cagatgatat agctttttac aataatcggc tcgccgccgg cggtggtgat ttcctcttcc
    39841 ggaacatcct caaacagacg tttgcgcgaa tcaaagcggt gaaccacctc gatcggcttg
    39901 ggcagtctga atttcagccc gggttctgtt tccggccgaa ccggcttgcc gaaccgcgtc
    39961 acaatgacgg tttctgtctc gcgtacctga tacagcacca tcgacagcag caggataaca
    40021 accaccacaa tcatgctcac aatgatccag aaatttttca ttggttctgt cccatttctc
    40081 tcaattactt tttgggttcc tcaagcccaa ggtccaaatc gtacagggaa gcggcaagct
    40141 tctcctgcaa atcgactata taaacctgag cgtcgtcctt ttctgccaca accacatatt
    40201 tccgtgcctt ttcgagggcc tcttccagca tcatcagccg ctgcagcttc ttgtagatca
    40261 gaggcgatgc ctgatacccc tgaatctgac cggcaaaccg aagcccttcg cctttggcca
    40321 gatgaatccg ctcgaacgca tcgctttcgg cctgccgcag ggtgctgaat accttgccgc
    40381 ggaccttcac aaacgccgcc tgcagctcct gacgcagctc ctgaattttt ggttcatctg
    40441 cctgcgcttt ggcgcgttcg aactccatcg ccaagtcata cagcgcatcg acatcggcta
    40501 tcgagccgcc cagttcggtg agaactttgt tcttttctgc ctgcgcactg agaatcgcgg
    40561 cctgcttctg ctggacagaa gcaaccacct cttcatactc tttggctact tccggcgggg
    40621 gatgcactcc ttgaagcccc agcagcacaa tctccacgcc cagctgagcc tcatcagcgg
    40681 ccgcctgaat ccgctgatgc agctcctgcg atgcttccat gcgtccgact cccagcagac
    40741 ttttccggtc tttgggcagc gcggaagcac tgccgtcatc cgtttcgatt ttggcgctga
    40801 cggcaaaccg ggttaactcg cggtagcaga tagcttccag aagctggcgg gcttctttat
    40861 gcttgtacat aaactggcgg agattcgaga tgcggtactg aatcggaata ttggcctgaa
    40921 ccacactgac cggaacagcc cctttatccc gcacagtgtc tgtttcgaca gccaccagca
    40981 gattgtattc ttcctcgtaa tgcttttccc cccacaaaaa agctttttta gccttgtctt
    41041 tttcgccttc tttgtagcca attgtaatct gctgtatctg gtcggttgga aagacataga
    41101 ccttttcaat tggccaaggc catttgagat gcagccccgg ctccaaatcc cttaccggtg
    41161 agccgaaata ttccaccacg gcggcctgac cggggccgac aaccgcaaac gaactcatca
    41221 aatacaatgt cagggccgca aataaaatca gcggcagaat ggcccgttcc agcaggcggt
    41281 aaaaccacgt ctgagagacc ttgaagccaa actgataatc aatcgtatgg gccaccgtgc
    41341 ggagaatccc gcccggctcg ttaatcagac cgatcaggcg gctgtcgtag gcagcccggc
    41401 tgtattcgcc ggcaatccgc ggacggtaaa tatccaaaac ggcattcaga agaatttcag
    41461 cccccagcac aaccagcagc cacggaatca ggtagctgag aatcaccagc ccctgcggat
    41521 acttaaattg actcaatgcc agtgaaaccg ccagtgcaaa gcccaaaacc gccgtcagca
    41581 gcagatagct gccgccggca cgcagcggct tccagtcctt ctcggaactc atccccgtgg
    41641 catagcgact gaacagaaaa ctgacaaacg agacaacaat catcagaact gcacccagca
    41701 gcgggttttt attcctccag tccaccggac cgaggacctt atactgaaac agcagcaggc
    41761 ccatcacaat ctggtaaacc gcaatcacta ccgccgcact ggggataatc cacttttcaa
    41821 aaaacgccag acgcttctgt gccaccgcca gcattgccag ccggtcggcc ccgccggtaa
    41881 aaatggtccc ctgctgggcg gaccgggaca gctgggtcat atccatcttt tcctgctccg
    41941 ccagcaagcg ctgatgaaac tggacaatca gcacagccca taccagcagc cctgccagta
    42001 tctgccagct gagcagatac atcgccagaa cttccgtaaa tgcgccaaat acaagcgtga
    42061 aaacaaagaa gaacaatcct aaacaaaagg ccccgatggc tgtatttctg gccttgcgcg
    42121 gtgactgatt catctgcatt tccctgtctt atctatatac caaccgaacc tttcttcata
    42181 gaagcagtaa agactataat aatttcgttc aaattgcaat gtccgtcttt acattttgct
    42241 tatgtgccgt aaaatattaa catgtctgca atgaaacagg tagtctaatt ttttaaggaa
    42301 aaaaaccttg ctgctgcgac ttcttgcaat cgccaaaaat acattcgttg aaacgctgcg
    42361 tcagccgatt tatgcggtca tcatcgtttt tgcactgctt ttaatgctgc tggcgcctgc
    42421 tatcagcatg tacacgctgg atgaagatat caaactcctg cgtgaattgg gactttctac
    42481 gctgtttctg gcgggtttgt tcattgctgt tttcgcctca acaggggcta tcaccgaaga
    42541 aatcgaaacc ggcaccgtta ccaccgtctt atccaaaccc atcagccggc cggtttttgt
    42601 attgggcaag ttcttcggca tccttgccgc cgtagcactg gctcattgcc tgattacaat
    42661 ggccttttta atggcagcca gacacggtgt tctggagacc gccagcgacg aaatcgactg
    42721 gacggtcatt gccgccgcca gtgcggcagc tctggctaca ttccttataa ccggtttaat
    42781 gaactatgtt tatgactgga atttcccctc gaccggtgtc gtcttgggca ccattttctc
    42841 cggctttgcc atcctttttc tggtctttgt ggacagggat tggaaattta atccccaaaa
    42901 caaccgcttc gagatgttcg acattgccgc ctctgccctg ctgctgatgg cgatgtccgg
    42961 gctggtggcg ctggcgattt tgttttcaac aaggtttaat gaggtcataa ccttgacctg
    43021 ctgtgtcggg attttcctgc tgggactcat aagcgactgg atcttcggcc ggtttgcatc
    43081 acagcatctt tgggctaaaa tcgcttatat tctgactccc aatctccaga tattttgggt
    43141 cagcgatgct atttacgaaa aaggcgctgt gccagccgcc tacttgctcc aaacagcaag
    43201 ctacacagcc ctttacacag ccggcatcct cgctttggcc atcgccctct tccagcgccg
    43261 tcaggttggt taatccagca acagttcacg attggcggtt tcggacagac aaccccggca
    43321 agagcctgct tgtgtcgccc tttcgaagcc gcttccaatc aatatggatt agtcaaccaa
    43381 ttcagccgct ctggaaaaat tgcgatgaaa atggggattt tttctcatca gtagattgtc
    43441 agcaatatat taacagacta ttaagatatt gttaacgact gaaaaacagc atccccattt
    43501 tccgacagcc aaagtaacca tttgccctcg atgctgcttg gctttctctg gacattcagc
    43561 tgccgttttt gcagatcgga gataatggaa ggtgtaatcc ggcaggcagc cgcaacattg
    43621 cgcagcaggg tgctaatcag caacagtccc ttgttgattg tgtatctttt ttgctgtttc
    43681 tttgatttac tggcttctgt ctggtgcttt cgaacctgtc cgtttgtggc cggctgacac
    43741 gatagaggag tttttagggt tgggctttta taatgtacat tccatagcga cttcatcgca
    43801 atggtcctgt ttggggcaag cggcgcagtc gctccagact ttcatcggca gcttatcccg
    43861 ctggacccgg acaaagccga gtttttcaaa aaacttgtcc tcaagggtca atgtaaagac
    43921 ctttttgagc cccaatcgtc gggccttgtc cagacagccc agaaccaatg ccttgccgat
    43981 tcctttgccg aagtaggccg gatccactgc aagagactta atttcagcca agttctccca
    44041 aagcacctga atggcacagc agccgacaac ccgtccctgt tcttcagcca ccataaaggt
    44101 cagcagccgt tcgtaaatat ccgaaacgga ggcaaacagc atgcggtcca cctctgcata
    44161 gctgttaatc agctcgtgga ttcgcttgac atctttaatt tgggcatccc gaataagcat
    44221 tgtgccggaa ttataaccct gtcggctgtg cgaatcaata aaatcttgcc tgcggaaaaa
    44281 tggctattta ttcagccggc cgttttggat acaatgccgg ccttatggca ttgccggcaa
    44341 aacaacttat gcagacggaa aaacagtatc gcctgaatct tcatccggcg gcattgaacc
    44401 gaacgattgc cgttctggcc gcaccggcaa ttttcgaaaa tctgttgttt tcgatggttt
    44461 tttttgcaga tacgctgatt gtcggctggc tgcgcaatga aaattatctg gcagcgaccg
    44521 ctctggcatc gttaaccatg ttctggacca atgcccccat tcaggcgatt tccatagcaa
    44581 cagtttctat tgtctcccgc acattgggtg aaaatgacta tgccagtgcc cgccggatcg
    44641 cgggccactg tttgattctg acttttgcgg ccgtctttgc aattctggcg gtcggctttc
    44701 cctttgcgga atggattatt cgcctgctca atgccgaacc gccggtgatt ccgctggcgg
    44761 cagcctatct tcgcatcgtg ctggtctcgt ccgttttcgg cctgccgctg atggtgtcca
    44821 acgccgttat ccgggccaaa ggcgacacag caacaccgat gtttattaca ctcatcatga
    44881 atctaatcaa tatcattgcc agcattcttc tggcattcgg atggggcccc attcctgcga
    44941 tgggtctcta tggagtcgca tggggcaccg ttctggcacg caatatcggc ggagccctct
    45001 cgctggccgt gctgatatcc agcaaacacg gcatcggact gcacatccgg gatttcgtgc
    45061 ctttacatcg gcggattttt gcacgaatct ggcatgtcgc ttacccggca atagcagaac
    45121 gggtgctcaa ttcgacatcc tatgccttct ttatgggcat ggttgccagc ttgggcacaa
    45181 ctgttctggc cgctcatcag attgccctca atgtggaatc gctggctttt atgcctgcct
    45241 tcgggatggg tgttgcgatt acaacaattt ttggacaggc cgttggttcc ggccgctggc
    45301 ggataggaca gattacagtc aaacgggcca ttatcttttc gggaacagct gggatactct
    45361 taagtctact gtttgccctt gccgccccct acggcgtcaa ggtctttcgc gccacgccgg
    45421 aggtcctgcg gctggccgga ttggctttgc agattgccgc tttggaattg ccgctgttta
    45481 cgctgacctt cgtgtttatg ggggccttgc ggggggccgg cgatacccgc agtccgctgt
    45541 atgtcaatgt ggcctgtatt ctgctgctgc gcctgccggc tacttggctg ctggcgtttg
    45601 tgctgaagat gggaattgcc ggcgtttgga tagccaccgc cttagactgg gcaggccgtt
    45661 ctttgggact ggggctggtc ttccgaagag gcatatggaa aaaaatccat catctggaaa
    45721 aacagaaatt tgccgaaacc gccatccccg tcagtcctga gccttaaaac cgcctgttca
    45781 ggccttcccc atccttaggg cgggatgatt tcaaaaggaa tagccggaat agcccccatc
    45841 gatatccgtt gcttttgggc tgccggactg cctgtgccgg ctgccggcgt ttcggtgtac
    45901 gcctcaacca acaaattgtc tgcaaatcct tctttgagca actctctatc cgctttcaaa
    45961 acgaatgaga tctcggatgc tttcgattta acttcttgca gcgtcagccc tgccggcggc
    46021 tcatgcagtg ccagctggat gttgttcccc tgcgccaacc ggggcggcaa tttcagcttg
    46081 acctctgtgc tgccccctgc cgggattttc attgggattt gaccgtccgg ctttatggcg
    46141 gacatacgct ggccggcccg cgggactgct gccaacagtt cctgcgaggg cagcaggtgt
    46201 ctgtacagga atgcctgcat cctgtcttct gcgggaacaa ctggacgggt aataatgctg
    46261 ccgccgatgc gggcgcgtgc gtccagccgg agagaaaccg gcttgtccaa ttttcttgcc
    46321 ggtgccgaca gagtcgccca tacagaattg cgtccctgcg gtattcggcc gccgctgatt
    46381 tcaaagccgg ctggagcatc cttcaataca atttcaatct ccccttcaaa accatcggtg
    46441 cgcagggcat aaaaacgcac cgctgccacc ccgccggcag gaacagtcaa actcgacggc
    46501 gtcatacgaa ccgcaaaatc gggcatcaga ccgctgatac gcagccgata gccataagca
    46561 gcgccgccgt gctgctgtgc atccgacaga tggacataat aagtccccga ggaagaaagt
    46621 acagccgtca catacgaatc ggcctgatgc gtcagcagcc ctgtgctgtc catgaacagc
    46681 cagccttcac cccagacagc aaagtcatca ttccaagcca aaacctttcc cgaggcatca
    46741 gtcagacgaa tcagagaatc caaaggagaa tgcagctggc gcgcacaaac ctgaatagca
    46801 atctcctgac cggcaagacc atcgaaacag tagatatcag cccggccggc ttgttctatc
    46861 tgaccattga caatcaccgg caactccacc ggctgggcat caccaagcgt gctgagggat
    46921 tccgtgcatt ccggcagtgt tcctacggca tacggtattt cattggacac ccccgcactg
    46981 ccctgacact gcaattgccg aatacagccg gcacaggggc ttgtatccag cggcattcga
    47041 tctgccggca gattccaccc cttgatttgg caggcagcag attctccttc tctggccccc
    47101 agcggaaata tctgggtaat aaacggcagc ggtcccacag cgatgcgata cacaaaatct
    47161 tcccggccgc gatacagaga atcccgtatc tccaattcat attcgccgct gcggggaacg
    47221 gtaaaaaata aaacaggatc gggattaaaa gaataatcat ccgcatacac aagttctctg
    47281 ccggcagaat catacagttt gataacggct tgaaaccagc ccggcacagc atcggccaga
    47341 taaggaatca gactgcgggc atgaaccttg gcaaccagct gcatgccggc gggggcagta
    47401 aagcaaaacc ggtcaacatc gccgggcata atctgcccgt ttatcagaac aggcagttcc
    47461 agcggctttt cagcaggtgc atccggcaca tccggcaaag gcggatacgg ctggcgattg
    47521 ttgggctcca gttcccggac ttcctgcaga gtgcctatct caaatgtgat cgggtcggtc
    47581 aatcctgccg acgttgacag ccgcagctca cgccggccgg gcggggcatc ggaagcagcg
    47641 gtaatttcca acagcaccgt ttccgaaatc tgccggttct gctgcagctt ggtgcggggg
    47701 aaaaacagca tgctacggat atgcgccagt tcccgaaggt ttttctgctc caacttgtac
    47761 agcagcggat gctcgggcat ctgcaccggc ggggcctcct tgttcggttt gtcttccgaa
    47821 gccgtcgctt ggggtgatgg cttggccgct gcatcatcgg ctgcggcttt gccggtcagt
    47881 tcctcaatcc gctttctgcg gacttcgtcc aaacggctgc ccagcaagtt ccgctgctct
    47941 ttctggagat tgcccagcgg ccggtaaaaa cgaataaccc tgccctgtac gccctgcccg
    48001 gagatataga ctgcatcggc accgcgaaga aactggccgc cggccgtaac caaaacggtg
    48061 ctgccctgct ttgcgccagc cggataaaga tagccgatat gcggctgact ggtgtttaac
    48121 tgtccccagc tcgctgccgc atgcagggtc agaagataca cgacaaatac tgttttcatc
    48181 ctcgtcattg tgcttgactt ctacataatc tctctgagcc ggccgccttc gctggattcc
    48241 ggcatcacct tgacatccag accgccggca ttgggcagcg gaccatcggg attgattccc
    48301 aaaagaagat aaatgctgcc aatcaaatcc tgcggataaa ccggacgctg tgccggctga
    48361 agcccttttt cgtccgacgc tccgacaaca cagccgcctt tgaagccccc gccggcaacg
    48421 acactgcaga agcttttacc ataatggccc cggcccccgt tccacggggc ttcccattga
    48481 atcttgggcg ttcggccaaa ttcgccgccc caccagacaa tggttgtatc cagcaatccc
    48541 ttatcggcta aatcctgcag cagggctgac atccccatgt ccagttcggg cagtttgcgg
    48601 cgcatgatat caaaatgctg cttgtgcgtg tcccatcctt tatagttaat ggttacatac
    48661 ggcaccccct gttcaaccag ccggcgagcc attagacagc attgcccaaa ggtattgcgc
    48721 ccatagcgct gacgaacctc ctctttttcc tctgacaaat caaacagccg gcgggccggg
    48781 ccgaacatga tagcgtaggc gttctgttca cattggtcca gccgtgcaaa ctgggggtca
    48841 tcaggcatca gtttacccaa tacatccaga gaatgaagaa actgccgtct gttttgctga
    48901 cgctggtcgg aaatgccctt ggccacaatt ccctcgacgg caaacggcgt ctggttcggg
    48961 tcgccgccgg ttacgaaggg cttataccgc tggcctaaaa aaccggcttc ggaaaaacgc
    49021 ccctgcgact ccgtcagcac aatataggga ggaacaatcc ccgtatagcc atggtcatat
    49081 cccttaaaca gcgaaaccac tgcaccgaca cttggataga ccagccggcc gggttcgcgg
    49141 cccgtctgaa ccagataaga ggccgtttcg tgattgttgt tgccgtgcgt catactgcgg
    49201 ataatcgcat atttatctgc ctgtttggcc agcagcggca gcagctggct gatttgtatc
    49261 ccgtcgacat tggtcggtat cggctggttc agcggtccgc agtaatcgta tccggcttca
    49321 ggtttgggat caaatgtatc cagatgcgat gggccgcccc acatccagat ttgtatcaca
    49381 gattgagcgc gggctttcgg cacagccggc ggatttgccc agagccggct gcaatgaaga
    49441 ctgacagcac cggcaagccc ctgctttaac aggtcccgcc gcgttaagcc ctttttgatt
    49501 ctctgtctat tcatcgatgt cccgcccgca ttgggttttc accggcttca atgccgataa
    49561 agaaattcct tcgtgttaat cagcgcccac accaaatcat taaccgcctg tgcgggtgtc
    49621 agcccttggg tattaaaata gtcctcgata attgaccgct ccgtctgttc aggaaaacgc
    49681 gacagaatcg tcagatatag tgtatcaacc agcatcctgc ggtttcgtcc ggccgcccgc
    49741 atcaattgcc gcagatgcgg cccgcgttcg attttgttct gaatatgaga ggaattcagc
    49801 agatgcagcc gctgggcatc cgtcggcaga ttggttcgtt cagactgcaa acccgtatca
    49861 cgtgcgggcc tgccgaacag ctcaagaaac gggctggtta tgctgctgtc agccaatgca
    49921 atactccgat tttcatccgg gataaaggta aacggttcgg gaacagggct ggagtagctt
    49981 tcctgtgttc cggtgatttg acacaatgca tccatcagga cttcggcatc aagctgacta
    50041 acaggataac aggcaaacag ccgctgtgca tcgggagact tcgcctgcgg gatacaggat
    50101 tgctgatagg tccgtgaatt cagtatcaaa cgatagatat gccgcaggtc atagccggac
    50161 ctgaccagct ctttttccag aaaggcaaga acgtccggat agaccggggg attgtccgga
    50221 ccgatatcat ccggcgggtc aataatgcca tgccccatca gccatgacca gatgcggttt
    50281 actgcacatc gggcaaacca cggattgtcg gcgcgtatca gccaattggc aaaaacctca
    50341 cgcggatcct gattgggctg gattaccgcc ggctgaccat ctggaaaaac acccgttaac
    50401 ggcccgcttt ctgctggctt tggatatact atctcttctt tccattcagc agtccctttg
    50461 tagccaatct gggaaaaaaa gacctctatc ccgctccggc gttcctgcgg ccacttctcc
    50521 agacgcactc ccataaatgc aagagccgcc gcagccgcaa cggccgacgg gtctcgatcc
    50581 tgaaccgccc gatagaagtt gacgggggga ttgcggaaat tgcttccgct ggatgtcaaa
    50641 agctgacgtg caaatgtgtc ataaggcata tttgctgcca gcgcatcgta aatccaccga
    50701 tgataggcct gcacagcatt gggccacagg ttgatgggga attctgactt gacccgcagc
    50761 aaatcacacc atttcaatgc tgtgtattga gcaaattcat ccgattccag cagggtattg
    50821 ataagcgctg cacgcttgtc ggaacgggga ttttccagaa atcgccgaac ttcttgcgca
    50881 tcgggcaaag tcccggcaat atccagatgc acgcggcgga taaaaaccga atcggagcac
    50941 aggttggccg gctttatccc ccgtttggac aattccgaca ggatacagct gtctatctca
    51001 ttgaccggtc taaaatccga cgaacgctca aaaattgaaa ccttgtcatt ttgctgattt
    51061 gactgttgcg atgaggcccc ataaacacct gcaatacacc aaacagccgc cagcatagta
    51121 aacagcggct tgctgcgaac aacctcgtac ttgtccagct ttttgtacat atccatccaa
    51181 acgcaatcgt cgatgctgct gtaaacaaaa cgcctcttag aactgaaatt attgcctgtg
    51241 tacagcaaac aaccagcggc agaaatcctc ctgcggttcg ccaaaagtta tctaagtact
    51301 tttttatgca agacttataa taccaatgat aaacttgcct ttcctgatat ttcctttggc
    51361 aggactgaaa cagtctctaa attcggcaag aaatatctat agacctgcgt tattttcttt
    51421 agttcgattc gttttgagaa gactttttta aaacttttgg gattatttta tgaaaattac
    51481 agacaaactg catagtattc ggatttcagg gccatctggg gcggagtatt ttcttcacgc
    51541 acaaaaccgc ttccccgcac aggcagcaga acttcttgcg gaattatctg cctttctgca
    51601 gcagaccgat gcccagcttg tttatcttcg gatttttgca caagaaaacg ttcgaacaac
    51661 ccttttgtcc tcctgcctgt cccaattgca gcgcctgtgc tgcccgataa tctggattag
    51721 gcaggacaat aacaattatg ctttcggatt gcagggctat gccgtcgaag gcacagacac
    51781 agcgccaata atggttgaca ataggcttgc gggacgatgc tttacaagcg gcggttgccg
    51841 ttattaccat ttcagtttta cgcctcaaaa tcctgacgcc ggccgatttg agcaggcggc
    51901 tgctcttttt gcccaaatgc agaaaacgct gtcttcggca ggactggatt tttcatcagc
    51961 cgtgcgtacg tggctgtttg cagaggatat cctgtcatgg tatagccagc ttaatcaggc
    52021 ccgcactgca ttttttaccg agcacaacat ttttcaaaaa cttgttcccg ccagcaccgg
    52081 agtcggcatc ggcaatctgc tcggcggcgc aatagccgca gaacttctgg ccgtaaaacc
    52141 cctcaatgga tgcgtccgga tagaacaaaa ggcatccccc cttcaatgcg aggcgctggc
    52201 ctataaaagc tcattcagcc gtgcagtcct gattgcatcc gtcagtgacc ggcggcttat
    52261 tgtttcagga accgcctcga ttgatcaaaa cggcctaacc gtttggccgg gcgattgtgc
    52321 aaagcagatt gagcaaacca tgcaggtagt ccatgccctt ctggacagca gcggtatgga
    52381 ttggaacgac accgtacggg ctattgcgta tttcaaaaac agtgcagatt tcggactgtt
    52441 tgatgcctac tgccaaagcc gcaatctctg cctgccgcat cttaaagtac aagccgatat
    52501 ctgccggccg gagcttgtgt ttgaaatcga attggatgct gtaagatgct gctgaaaata
    52561 tcagaagctg cccgcttttt ttactcagca cacaacagcc agtcatcggc aaagaccgtc
    52621 aaatcctgcg cattgacagc ataatctttg gacaaatcga tgttgtaatc gcagacatca
    52681 gccgcattgt ccgtccgcag cgaccagtaa ttcgaaaatg cagcaaaatc gtccagattg
    52741 acaatgccgt cgccgttgat atccgcccgc aaaatcggcc agacaatatg caatgcatcc
    52801 aaaccaaagt ccgccagatc ctctatcggt ttaaacggtg ctgtcaattc ccggttgcgg
    52861 gcaatgatgc ggcccgtatc gctgagcaga tcgccgtggc caatccagcc aaaccgcttg
    52921 tcggcaaaat ccttttccgt agaaaaccag atctcaccat gcggccatac atagaccgca
    52981 tccagaccaa catcttgata ttccccgacg gatgattgtg ccgtttcgaa ctgcttgagc
    53041 agctgggcat ttgtcctgta aatcctgccg tcttcagaca gtaaatctcc cggactgatc
    53101 atcacgccca aagattccga gaagaatcct ttctcaacag aaaacagcag tcttccggac
    53161 ggactgaatg tgacagcatc cagaccataa tccggcaccg gcggctgcgg gcaaaacgga
    53221 cgaataaaat ctgtaaagcg ccggacaata aagccggctt cactcagcag gtccccctga
    53281 tgcaggggcc caaggatttc gctgaaaata tcctgctcca tcgaaaacca gaatgtccgg
    53341 catttgcagt ccctgcttgt taactgcggc catcgcgctc ctattacggc atctaaaccc
    53401 acatcgggca ccagcggcat aatacccagc cggctggtaa gctgagcatt ggtgcgaaga
    53461 atccggccgt ggactgtcaa taaatctcca tcactgatgt acgttttgtc caatacggtt
    53521 cgtaccgacg gatgaaagcc cgctgcggtt gaaaatccga cctgcggcca tggaacagct
    53581 gtcagatgca ggcgaaacac atgcagaggg tcatccggct tgcactgttc taactcaatc
    53641 tctatccacg gcagacccat cgttggaaaa accacgccgc tgtcgaatga caacccctga
    53701 ttattgttga tgcttccctg cagcgtcatc cgatgcacaa aagcaaaatc gcccccgatt
    53761 tcataatcgc cttccaacag tccgaaatag cgaacgtcgg ttctgctgct gttaaaacgc
    53821 agattccgta cagcaaaatg cagaaaccac atatccggct ctttgggaac cagccagaag
    53881 gtgccgcgta taggaacagg aacagacaga cggtcgcaaa acaggcattc atccagaagc
    53941 gtactgcctt caataagccg ataccgaaca ggcccatttt tcaaaagcac cgatgccccg
    54001 gaggcagctg cctggttgtt aaaactatca gggtattcat ctgcaaaagc cgctgccgtc
    54061 agcaggcatg ccaaaactat ccagaggaag tacactgtgg caaagaagtg gtatttcgct
    54121 ttcatcggtt taccctccaa atgctttact cttttcggca aatccgataa ttaaattata
    54181 agacagacgg ctgcaaaaaa tcaatcttta tttctgcctg ctctcaaaca cctgtttaat
    54241 aatcttaaaa caggaatgct caaagccggc tgtttatgaa aagtacaagg atatatgtgg
    54301 gtgttttata tccaatacat taattgcccg cagatgaatc aggttccaga gcagactttt
    54361 ttttcgtccc agcataaatt tcatacttta aaagtctgca ttccaaacct ccattgaaaa
    54421 aggtcagccg ccggctggta cgcagaccaa cctttttagc caattccaga ttgcccgtga
    54481 agatataccc tgtatatccg atacatttct gcttgaaaaa atctccgatg cgggcatagg
    54541 tttcctctaa ttccttgaca tcgcccatcc gcatcccata ttcaggattc atcaggatta
    54601 ttcccggttc ctgcggaact ggtgtttgag caaaatcgca gacagaaaac tcaatcaagt
    54661 gctcaacgcc tgctgtctgt gcgttgcgtc tggcggcctc aacggcccaa gaatctatat
    54721 ccgtcgcaat aataggggcc ggccggtgct gggctttgcc gccggttttt cggcttttct
    54781 tgcctgcttc ggctcgcaag gcatgccacg ccaaggcatc ataccacttg gtatgcataa
    54841 agctgaaatt gctccgcagc agtgccggaa cacgccctga agccgtcagc gccgcttcaa
    54901 tcggcagtgt gccgctgccg cacatcggac agaccagcgg ctgcgtgccg tcgtatccga
    54961 cagccattat aatcgcagct gccagcgatt cacgcatggg ggcatcgatc atcgttttgc
    55021 gatagctgcg gtcggacagc tttcgtcccg atgtgtttag atacaccttg caggttttgc
    55081 cgcgccaaaa aagctgaatc acaacattgt cgcgttcctt gccggaattg ggacgcgagc
    55141 cgcactgttt gaggatccgg tctgctatgg catctttaac ccgcaatccg gcaaacattg
    55201 tgttgtcgat agatggagta tccacgcggg taacgacgct gaaatattcc gccggatcaa
    55261 taagctcttc ccaaggcatc tggcgaattt tgcggtacag ctcatcgggc gtatcgcacg
    55321 gaaacgaccg cagtaggtac aggacattat atgccgttcg cagatagaga ttgagccgca
    55381 tcgcatcgaa catatcccct tctatcgccg cgtcaactga tccggccgcc gtaatcgtat
    55441 agcccagctg ttcaatttcg ctgcgcagat agccaaccag ccccggaccg caggtaacca
    55501 gtatcgtgct tttttgactg ggcttcacgg attcatgcca gactttcaaa taccgctcta
    55561 tctgcggcag tttatactat aatggatttg catcggttac acattgaagc ggaaattgat
    55621 gatgtcgccg tcctgaacga tataggattt gccttccagc cgcattttgc cggctgcccg
    55681 gacggccttc tcatcgccca atcggcggag gtcttcatag ctgattgttt cggcacggat
    55741 aaaaccgcgt ttgatgtcgc tgtgcacctt tccggcagca tcaacagccg ctgtgccttt
    55801 ttcaatcggc caagcacgca cctcatcctt gccgacggtc aggaaactga taaggcccag
    55861 cgtgctgtaa cagcattgga caaactgctt ggaagccggc tgcgcaagcc ccatctcttt
    55921 cataaaatct gcccggctgt cagggtccag tttggccagt tcgcattcca gctttgcaca
    55981 cagtcggatg accggcactc cttcgattcc gatgctgctg ccgccgtttt gacccagacg
    56041 gttttcgccg acattgacaa caaccatcag gggcttgagg gtcagaaagt tcagcgaccg
    56101 caccatctcc atctggggac catcggattc ctgcagcacg ctgcgcagcg gcttttccgc
    56161 ttcaatcgcc gcctgcagct tgagctgcag ctccagttcg gctttatcat gggcctgcgt
    56221 tttagtgggc ttgtgtgcct gcttctggag ccgatcgatg cgcgttgtga ccatttccaa
    56281 atcggccagc agcatttccg ttttgagttc ttccaaatcg cgcaccggat cgacactgcc
    56341 gcgataggcc ggcacagaag gactgtcaaa atcacgaatc accagaacga acatgtccgc
    56401 cgtccggatc tgaccaaaca gccgccgtgc cgccgcccga ccatgctcat ccgcaaacga
    56461 aagccccggc aagtcaagac agtccaccgt ggcatgaaca gtctttttgg gctgatacag
    56521 ttctgtcagc cagtccagcc ggtcatcggg aacagaaaca atgtgctcgt gaatatccgg
    56581 cgaacccatc ggagcgggct ctttgccgct aatcgctgaa agaagcgtgc ttttacccga
    56641 ctgcatcaaa cctattaagg caactttcaa tggctgactc ctgttaataa tggttacctg
    56701 tcatatacag attttcccca gcagtcatag gcaacaacgg ctggaaaatc cttaaattcc
    56761 agcaggcgga cggcttcggg gccgaggtcc tcataggccg caatctcatt gcggacaata
    56821 tgcctgctca gcagcgctcc tgccccgccg agggctgaaa aatgtacccc accgtactta
    56881 accagtgcat cccgcacggc ctgactgcga tatcccttgc cgatagttcc cttcaagcca
    56941 tacgccagca gcatcggcgt aaaggcatcc atccttgaag aggtcgtcgg cccagcagcg
    57001 ccgataattt tcccgggcgg tgccggtgtc ggccccacaa aataaatcaa cgccccctcg
    57061 atagccgccg gaagcggtct tccctgtgca atacagtcac agaatcgctt gtgcgcctga
    57121 tcacgggcgg caaaaacagt gccgcaaata agcacctcat ctccggcgtg tagatgctgt
    57181 accacagcat tatccagcgg cgttgttatg attttcttgt ttgtcatatg gttttgggaa
    57241 tacgattcgg ctctgttctt cattgtatag aagaatactc ttaaatccag caaaaaagtc
    57301 tggatttgac tgcgatattc cgcccaaccg gtgtaaaaaa ggttgcatca gcagccgctg
    57361 cgtggtatga tgaaggtatg gcaaaatatc caatttatct tgaattgagc gggaggcgtg
    57421 ccgtagttgt ggggggcgga gcagtagccg tacgaaaggt gcaggccttg caggaggctg
    57481 gtgcacgcgt aattgttgtc gcagagtacg ttcatccttc tcatgcagat gcgttgcggc
    57541 ttccaaatgt ggaactgatt atcagcccgt acagaaaaga ctatctggcc ggctcaacgc
    57601 tggctatcgc ggctacaaat gacccggcgc tgaatgagca gatctttcac gattgtcaag
    57661 agctggaagt tctgtgcaat gtagtggaca agcccgaatt gtgtgatttc tatacccctg
    57721 cggtcgtcaa acgaggcgac ctgcagattg ccgtctgtac agaaggagac tgccctgcct
    57781 atgctggtca tatacgcaga aaactggagg aacttttcac agaaacacac ggtcattttg
    57841 tcaagcaatt ggagaaggtc cgtcagcaaa tcataatgga aataccggat gccgaccagc
    57901 ggaaatcgct aatggggctt ctggccagcg atgaatcctt cgagattttc aggcatcagg
    57961 gacagcagaa gtggaatgca tatgtcctcg ctcagctggc cggaagcaaa accgcctgac
    58021 aatgcaatcc ccagccgtca tttactgcca atgaaatgcc tgacacggga aatctggatg
    58081 aatgtgccgc agcgccgggc gattgtttca atccaccgcc aaattgaaca attggttgaa
    58141 gaaagcggcg tcagggaagg gcttgtcttg attaacgcaa tgcatattac cgcctcggta
    58201 tttatcaacg acaatgaacc cggattgcac catgattatg aagtctggct ggaaaaaatc
    58261 gcccccgaaa agccgtacag ccagtatcag cataacggct gtgaagacaa tgccgatgca
    58321 catctgaaac ggcaggtgat gggccgtgaa gcggtggttg caatcaccgc cggcaagctg
    58381 catttggggc cgtgggaaca tattttctat tatgaattcg atgggatgcg caagaaacgc
    58441 attctggtca aaatcatcgg cgagtaatga cagaacttct gctgttttta attcaggccg
    58501 ttatcatttc gctgtccggc gtgatggctc cgggagcgat gaccgctgcg gcaatcgctc
    58561 agggaacccg caatcgctgg gctggaatat tgatttccat cggccacggg gcggtggaga
    58621 ttccgctgat ttttctgctg atgctggggc tgcattttct gtttgagatg ccggccgtgc
    58681 agaaagtggt cggtcttctg ggcggggcct ttctgctgtg gatgggctat ggcttgctcc
    58741 gtcagtccgg acagccccgg acgccgcagc atcccagcgg gcattccgca ctcagctcca
    58801 ttggagcagg cattgttctg tcggctgcca atccttattt tctgttgtgg tgggcgaccg
    58861 tggggctgaa tctggcttta ggcgccaaaa atctgggctt tgccgccttg gtgttgtttg
    58921 cagctgtcca ctggctgtgc gacttggttt ggcttttcct tctttccttc gccgcttttt
    58981 acaccaataa aagagcaggc ctgtggggcc ggccggccga aaaagccatt cttttattct
    59041 gcgggtcggc actgctgttg ttcggcatca agtttatata cagcgccctc atgccggctt
    59101 aatcggaagt gcctgacccg gcatcggtct gtttgtctgc ctgataatca aaccgctcct
    59161 ctttagccag aacctgcttg agcgtataac gcgacagatg cgactgcatt tcctcaaaca
    59221 cctgctgcat cgctgtctcc tcgctgccgg gctgggcaaa actgacttca ctgatagacc
    59281 gcgagatttc atccagctta atatgtgcac catcggtgga tggcacatag ccgacgctgg
    59341 gttcgatggt atggcacaga agccctgaat gcaccagatg gtcaaggatt tttcccgcaa
    59401 aatccaccgg catcccaaat cgccacgcaa cggcctcaac actgaccggc ttctggtcct
    59461 tccgctcaaa ggcattgagc acatactgca tcagccgaat caccgtttgg tcattggcca
    59521 gaaaatactg ctgacgccgc cgggttttct gaaattcagc agcgtcgaga tgcttgatat
    59581 tctgggcagc ataggtcagc tgaagcccga aaagaacgat cagccaagac agaaaaatcc
    59641 aaaataccgt cagaggaata atgccgagaa tgccgtaaat cgcctgatac aaaaccacct
    59701 tgctcatata cacgcgcagg ccgtatttgg ccgccgtcca cagaatcgtg gccacaaaag
    59761 ccccccacaa agccgcaccg gggctgatgc gggtattcgg cagaaaataa tacaaaaaga
    59821 aaaaacccac aatactgatt gcaaacggaa tagtcggccc gaggaaggca aagattccct
    59881 gtgaaaactc atgaaccagc agatagcggg cactgacata cagccccgct ccaatcaaaa
    59941 gcggccccag cgtcagcagc gcccagtagt taaacagacg atgcagaaaa gaccgccctg
    60001 tcggcacatg ataaatagta ttaaaggttc tttcaatcgt ggttaacagg ccgatagccg
    60061 cccagataac cagcaccagc ccaacgcctg ttatggcgcc ggtattaagc ttggacatat
    60121 agcgttcaat caattcatcc agcttggccg ctacagtaat ctttccgccg gcagcgggaa
    60181 ccggcgaccc ggccgccgca gcggcagggc cggagccatc gggcggcaat tctgtttctt
    60241 ccaccggata ttcaatatgg gtcagattca aggattcgta aaggagcttc ttaacccgtt
    60301 ctcctgcttc gcgattggct ggaaacatct gaaaaatcat caggataaca atggccagcg
    60361 gcacaagacc gaaaatagaa tgatacgcca gcgccgctgc ctgtgtcagg cagtggttga
    60421 ccccgagcag ccgaaagcag tgaaaccaaa cgcgtacctg cagagccaga aaccggcttc
    60481 ccttgcccag tccctgcgtg ggcagggtga aaagctgacg cagtttctgg ataaaaaccg
    60541 gttcataact catctgaaga tcctttccag cccgcgctga atttccttct ccagcagctg
    60601 ctgcccctgc ttttcaatca gcttggcagg attgatttta ggacgaagca gcgatccttc
    60661 aatcggaacg gtgactgtat ttttatccac aggaatctcg acatccatcg tcagcgtttt
    60721 atccagcccg actttccccc taaaatgaag cggcgtattt cccacatcca cctgcatatc
    60781 cttataactg acaacacctt tttcgaccga aaaatcggtc ggtcgaacag tcagcatcac
    60841 ggaatcgccg gcattcagca gcgtcagcag ctggccaaga aaatcacccg cctgcattct
    60901 gagcgattcg atgccgaatg ttcccttgag cgtcagcagc atcatatcct tgcccgacag
    60961 cggaagaacc atctgctgtg agctgaagct cagtatgccg ctggtcttga cagcgttggc
    61021 aaaaatcgga ctggtataca gcagcatggc ctgtgaaagg gtatcattga gcgcaatctt
    61081 atccaaaatt gtcatcggct gcttgaggcg aaacagaagc ggctcttccg ccagattcat
    61141 cgtcccggca aactgaagct ttccgccgtt gacagtggtc tggggaatcg tcagatccac
    61201 aagccccttg acaatattca gcttcacatc ggttggaccg aaattgagac cgaaatagct
    61261 ggcgctgtca aaacccacag atccggctgc attgagattg gccttcatca attcgggctt
    61321 gtcggtcgga tattcggaat caaaatgaaa tgtatccttg cgcgctcctt ttaccgacag
    61381 tccttccggc agatacattg aagcaaatgc agaaagcgcc tgccagtcat agtgagcctc
    61441 cacgtcccct gatatccggg tcagactgct gctgactttt tttttaactt cccctttggt
    61501 gatgctgata agcgtctggc ccgcagcccc tttcaggtca aaagccccaa tatctatcgt
    61561 ctgctgttct gtatccagaa caatatcgca tttcagcgtt atccggtcct gaataaacgg
    61621 ttcgccggct ttcgggatga tttttaagcc gtcaactttt gtattttcgg actgcagccg
    61681 catctgcgaa cccgccgtag ccgctttcag cgtcgcatcc agcagaccgg ctatctgcat
    61741 cccttccggc acctcggcaa aaacttgagc aaacggccat gccttggcaa ggtcaatctg
    61801 tgcgctggca tctgctgaga tagtcttttc ccc
//
LOCUS       DAOTBO010000004        57486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_442_length_57486_cov_5.541171, whole genome shotgun sequence.
ACCESSION   DAOTBO010000004 DAOTBO010000000
VERSION     DAOTBO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 57486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57486
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_57486_cov_5.541171"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLE88_00935"
     CDS             <1..200
                     /locus_tag="PLE88_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75063.1"
                     /translation="NYGLSAETIGGQYYGVTGIRPCIDPAFAGSIYNRDNTGSSYCKV
                     DLADFALLAANWLADGLWVAP"
     gene            306..1001
                     /locus_tag="PLE88_00940"
     CDS             306..1001
                     /locus_tag="PLE88_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75064.1"
                     /translation="MKRLGILLLLGMIGAAQAVLIDDFSGDLSAYTSTVILDANGGGA
                     NTAAWQISGGTLQLNTTVYDGIEQYAMIYSGLTLGIGQELQADVVIGATGSQDIGLYV
                     GGTTPTTGVRRDYIAMYRRSSGQLFSRGFDGTTEYTLAGNWTNNIPITALFIARIAEN
                     TYEAGWYDGATRNVLVTRTPAYANDADVIGFCADVRGVGIVGSLDNLRIVPEPATLTL
                     LGLGTGLLARKRK"
     gene            1222..2766
                     /locus_tag="PLE88_00945"
     CDS             1222..2766
                     /locus_tag="PLE88_00945"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HOM75065.1"
                     /translation="MPIHTTELKYIGRHLFIFYLFLYSVCLAFPTAVKIQDTVVDTQA
                     MTLGAGAPYGYAINGLSFQQEALVTFNGWQYAVYYNSSRRVCLARRQLPSGAWSVIPF
                     TDYTLSTTTDAHNVVTIGICPNDGTIHLSFDHHGGSLHYRVSQPGAASHPDSVVWNAA
                     LFGPVRSYLIAGQTITSVTYPRFWQTPDGNLQFGFRAGASGNGDWYMADYTADDGLWS
                     NVRQVINRSGTYTDSLGTSTTRNAYMNPLVYGPDGILHATWTWRESAGGANHDIVYAW
                     SPDGGFHWYNNNVAAGFRIGTGSSPLQTLLSVTWINEGLQLIGKATGSTTTQQLITVH
                     SSNAAVIAVDRYYGIMNQQAQAVDAQGRIHTIMFHCTPETYEGYSYSTWGPQGARRYY
                     HYWRNAKGIWTRNELPGWVGSRPKLFIRSNGDAYVIYQSRRNVNPTDTGIYFTDGDLT
                     IQAATAASSWTDWQIIHIEPGYFFSEALADPYRFQDGVLSVVMQESPSTIGGASRLRI
                     LDFELN"
     gene            2905..4566
                     /locus_tag="PLE88_00950"
     CDS             2905..4566
                     /locus_tag="PLE88_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GC-type dockerin domain-anchored protein"
                     /protein_id="HOM75066.1"
                     /translation="MPTQGSPLIGKEADGNQYIESFGSYCLLGDHAVPAQDSATTVFY
                     RIYKPAGTSPDCSVGLSDLSAPTGDWNDFEVYVVIVGGELRARNGTSNTAIISPMNSG
                     VWYNIWLILNNSSNTYDVYVTNGSASATASDRKASNFVFRKASSNPLAAFKIYGRPEY
                     GPVWVDDIYITNGIDLTNPAAAVSPLIIHNPSNSSIKQSEEAIFTTVFACQTSPSTIW
                     YKAASPADIPIDTSLPAFDLQLDYDPLTRHYTSTLKILDCTVSNSGYYYCKIFDESGY
                     SRSSASALLIVYGLSAHWTLNKDRYISGSYLDELAGLPAYAAGACSFTNGADGTPDGA
                     GLISPSGGWAESSIFDPVAQSGQMTVSFWAKTSDWGTSQEDLLAESISDEILIVPNGL
                     KADGRWQHIGIVFDGTTGRLYVDGLLQAQGLWKMPQDTTAVIRFGTAGTGQSIFSGAI
                     DDIRVYNYALTEFEIADLRYDFSGISSCILDFDAHYDFAGPDGTSDCIIDILDLAAFA
                     AAFLEADPARDLTGPDGLPDGIVDLQEFAEFASIWLACGLYPVCN"
     gene            complement(4598..5332)
                     /gene="rpoD"
                     /locus_tag="PLE88_00955"
                     /pseudo
     CDS             complement(4598..5332)
                     /gene="rpoD"
                     /locus_tag="PLE88_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(5888..6490)
                     /locus_tag="PLE88_00960"
                     /pseudo
     CDS             complement(5888..6490)
                     /locus_tag="PLE88_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            complement(6542..8341)
                     /gene="dnaG"
                     /locus_tag="PLE88_00965"
     CDS             complement(6542..8341)
                     /gene="dnaG"
                     /locus_tag="PLE88_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOM75067.1"
                     /translation="MAGKWEFSLISRIQQANDIVDVISEHLSLEKRGKELVGLCPFHA
                     DHKPSLYVSPAKQIFKCFACGAGGDVLKFVQMKENLTFPQSVERLAERAGIRIEPLRK
                     RNVGASGSLLDAVSMAKLNNWAMAHWVSNLHHPEKGIPGRTYLTGRKINEASIKKWQI
                     GLALDAWDDLAAAAAARKIGCKHLVEAGLAVEKEAGGCYDKFRNRLMFPIVDVTNRVI
                     GFGGRTLGGDPAKYMNSPATVLFEKSQSMYGLNHARQEITQTGIAVVVEGYTDVIMAH
                     QFGICNVVAALGTSLTDSHAKLLKRYCKRIVLIFDSDTAGRAAANRALEICLAEKVDL
                     RLGFVPTGKDPCDYLLEAGADAFRHVIDNAVDVLEFKWELLEEQLNAGQNLADKAEAI
                     RQFLQTAASAFSARQMDSVTRAVVLARLSALLQIPVGRIEEEIHKIQKQNQRIQRKYE
                     TETSAKSLGQDLIIRTQREILEVLLQEPTYYPAVKERLCLDDFGTSGPIREIAEAFFG
                     LAEEGLEPSLARLYARLESSETAVILAEMDDAGHGKGDSRLRLEQALKVLEDQKNQLK
                     IEEKKGYLDREEVLKDMLKQIAHKGPDLRKAGI"
     gene            complement(8619..9305)
                     /locus_tag="PLE88_00970"
     CDS             complement(8619..9305)
                     /locus_tag="PLE88_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOM75068.1"
                     /translation="MLKAVIFDFDGVICDSELLHYKALNRVFNRYGVDVPKEVHWAKY
                     LGYSDLENIEAVNRDYKMGWDAAVINRLMKEKKILFEELAGQESIIIDGVETFVRRLM
                     DAGIRCAICSGALRSDIELMLRGADFAKAFEVIVSADDVRYGKPHPEGYILTLKRLNQ
                     NSNTIINPYECVVIEDSHWGLEAATAAGMHPVAVTTTYPAEQLNGKAQIIVNRLDELN
                     MDDMENICRN"
     gene            complement(9453..10322)
                     /locus_tag="PLE88_00975"
     CDS             complement(9453..10322)
                     /locus_tag="PLE88_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75069.1"
                     /translation="MNKRSFTLIELLVVIAIIALLLSIILPAAAKAKDYVKRTICLNN
                     MRQAGLGVHLYAQSNDNQIMPLYTISTATIANPPSFIPLPYNSYMVYHPNQTKPGGGF
                     RPFHLAVLYDQHYIGDPKVFYCPAQPRTTRHYVIPYYYEFYIGQGDPADYFNPADKGS
                     YEWGKRTPADPRGASSQLVRTSFNYWNYGYSKIEKIAGFKPLIFDNIQDWRVVPHRKS
                     RDINSIPQGLSVLYADNHAAFCNDSYIFEETAANWPWNRAGSTSAAPTDVGQGPGNFS
                     DRFDEILRRIQAR"
     gene            complement(10530..12320)
                     /locus_tag="PLE88_00980"
     CDS             complement(10530..12320)
                     /locus_tag="PLE88_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOM75070.1"
                     /translation="MGNFYTDNDDIQFLFRHLNLAEIAELQEEGFKYAGQYDTAPVDA
                     QEAITNYDLVLRAVGQLSADFIAPRSESIDREGTTLNEDGSVSYAKGIAESLNALAKA
                     DVMGFTLPHRFGGLNFPNLIYSMAIEVVSRADASLMNIFGLQGIAETINYFADEEIKQ
                     RYLHDFAAGKVTGAMVLTEPDAGSDLQACKLRAFQDDEGQWYLHGVKRFITNGCGEVL
                     LVLARSEPDRSGGLGLSLFVCDRGPTVKVRRLEDKLGIHGSPTCELYFDNTPCQLIGE
                     RQRGLVTYVMSLMNGARIGIAGQSMGIAEAAYRVARDYAASRQQFGMPIEKLPAVRDM
                     LIRMKMEIEAGRALLYETSWVVDHEIGWSKIAEAGSDKDKVKEAKQQLKTVKRLAGML
                     TPMSKYYCSEMCNRITYDAIQILGGSGYMRDYACERYARDARITTIYEGTSQLQIVAA
                     VRGVCGGACEKYLADLSARSYVDSVTDLLALLSDGSQMLLKCVAFTKEQGVEYMDLYG
                     RQLVDMAIALINGYLLCGQASSSVKMLVRTAGGSDAVIPMIQRKEALARRYVTRSAAM
                     IKSLAEEVLSGDRSTFKQYEVLAGPVPEQI"
     gene            complement(12364..13392)
                     /locus_tag="PLE88_00985"
     CDS             complement(12364..13392)
                     /locus_tag="PLE88_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOM75071.1"
                     /translation="MIEVNKQGEVWIFAEQHKGHLEDTSLELLSKGRQLAAQLKVPLA
                     AVLLGDRISHLCEPLGHYGADKVYLAEHPLLEHYQTSSYAKVIDGLIHKYKPQIVLYG
                     ATPCGRDLAPRIASAVKAGLTADCTDLQIGHHEIKKTGQIFENLLLQIRPAFGGNIIA
                     TIINYDRWPQMATVREGVMPMPIPDGTRKAAVVKETVHLSHSDLAVEILSEHVQQKKV
                     NLKASRVVVAGGAGVGSKDNFRLIWDLANCLGGAPGATRAAVDLGFIDRDHQVGQTGT
                     TVRPSLYIAIGISGAIQHQAGMADSQKIIAINNDPDAPIFNIAHYKIVGDLNQVVPMM
                     IKAIKEKV"
     gene            complement(13453..14340)
                     /locus_tag="PLE88_00990"
     CDS             complement(13453..14340)
                     /locus_tag="PLE88_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOM75072.1"
                     /translation="MGYNCVVLVKQVPDTKRITGQAMNEDGTVNRAALPAIFNPEDLN
                     ALELALQIKDQYGGTVTVMTMGLPAAADVLREGLYRGADRAVLITDRRCAASDTLATS
                     YILSCGVKMLHPDIVLCGRQAIDGDTAQVGPQVAEKLNLPQITYVEALLSLDGKTIVA
                     KRNLGNGWQEVKSRLPVLLTVIDAANEPRVPRAKQLMKYKWAKCRIEVEAAAQSNPSV
                     DVEAVCRDLQTRGLLIDQWDLDYLKADLQWCGRDGSPTKVHRIQSVVLSAKESKTIEP
                     TQSGIDAMIHELIVDHTIG"
     gene            complement(14542..15885)
                     /gene="accC"
                     /locus_tag="PLE88_00995"
     CDS             complement(14542..15885)
                     /gene="accC"
                     /locus_tag="PLE88_00995"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOM75073.1"
                     /translation="MFSRILVANRGEIALRIIRACHELGIEAVAVYSEADREASYVKV
                     ADEAICIGPAAPAKSYLNIPAIISAAEIADVDAIHPGYGFLAENAHFAEVCTDCGITF
                     IGPDAAAMRLLGDKVAARDLARKARVPCVPGSDGEVRDEAEAVKLANKIGYPVIIKAA
                     AGGGGRGMRVAHNDISLHKGFSAARMEAEAAFKNGAVYLEKFIQEPRHVEVQLIADKS
                     GNAVHFYERDCTIQRRHQKLIEESPCPVLDEKTREELCKAGLRLIKEAKYHNAATVEF
                     LLDKDKNFYFIEVNTRIQVEHPVTELVTGCDLIKLQIKVAAGQTLELRQRDIVHRGCA
                     IECRINAEDPARGYAPCPGKIEKFRIPGGFGVRVDTHIYQGATVSPYYDSMIGKLIVH
                     QPTRQEAIATMKRALREFQIEPIKTTIPAHLEILSHNLFVKNQVDTHFIERHLSS"
     gene            complement(15932..16396)
                     /gene="accB"
                     /locus_tag="PLE88_01000"
     CDS             complement(15932..16396)
                     /gene="accB"
                     /locus_tag="PLE88_01000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOM75074.1"
                     /translation="MSDKKSMDIKKIKELIDLMKENDLIELEIVDDDSKVHLKRPGAE
                     APVLHPAPAQPVMAAPAPSVVSVPPAPAEDATLLKITSPIVGTFYQAPSPDSEPYVKV
                     GGKVNPDTVVCIIEAMKVMNEIKAEVSGTVVECCCKDGQAVEYGQVLFKVRP"
     gene            16649..18508
                     /locus_tag="PLE88_01005"
     CDS             16649..18508
                     /locus_tag="PLE88_01005"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOM75075.1"
                     /translation="MSKVNFIERLKSGIVIGDGAMGTMLYQHGVFVNTCFDELNLTKP
                     DLVGRIHSDYVRAGCDFIETNSFGANAFKLSRFGLVEDTVRINTAAVEIAKTVAGEGV
                     LVAGSIGPSGINNPAPDNELQRSLLEAFSVQAQALASSGADFLILETFTSTSELLIAI
                     EAASKTGLPITAQLTCTEQFETFFGTPIEKAISHISAHNAVTAVGLNCSIGPSDMLEA
                     LKRLRQITDKPLSIQPNAGLPRRIEGRTLYMCTPEYMAEYAKRFFENGARIIGGCCGT
                     TPAHIAEIVKAVRPLDKAAAAKPVPIQITIKDSKPAGMKPIPLIEKSRLGKKLAAGKP
                     ITCIELTPPRGTDTQKVIENAKLCAAYGIDAINIPDGPRASARLSPLVTALKIEQATD
                     IETILHICCRDKNLIGLQSDLLGIHAMGLRNVLLITGDPPKLGEYPDATGVFDLDSVA
                     LTRVAAGLNRGLDIAQNELPQQLSLTIGVGANPAASELHREIDRFRQKAAAGAEYAIT
                     QPVFDTEMFFAFAAAVEDCRLPFIAGIWPFTSYKNAEFMANEVPGVTVPDALLKRMAK
                     AKTREDGRRIGIEIAHEMIAVLAPHVAGFAVSAPLGNVKIALAALGRISLNEL"
     gene            complement(18582..19859)
                     /gene="lysA"
                     /locus_tag="PLE88_01010"
     CDS             complement(18582..19859)
                     /gene="lysA"
                     /locus_tag="PLE88_01010"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOM75076.1"
                     /translation="MDSFIYKKGKLFVEGVDVTKIAQQVGTPVYIYSKATILDHLRKI
                     QTAYAGIDTTVCYSIKACGNINILRLMAQAGSGFDIVSGGELYRAVQAGADMKKVVFA
                     GVGKTDKEILEALDAGIAYFNIESEAELENLIQLCRQCGKKTKAALRINPDIRYDSHR
                     HITTGVKETKFGVDIERAQKVFDQYAGNGVVELSAVHVHLGSGGKTIDPYVSGVKKIL
                     PLIDILRAKGYKIDTLDLGGGYGADYETDTVPSAADYASGIVPLLKEKSLKLILEPGK
                     SIIANAAILLTQTLYKKTGGQKTFVIVDAGMNDLIRPCLYEAFHFIWPVQVDDKFVPE
                     KRSKDMEMPGTEIVDIVGPICESTDYFAKDRALPPVKRGDLIAIFTAGAYGFSMASNY
                     NARLLPAEVLVDGDTFSVIRKRQTYDDLVALEK"
     gene            complement(19926..20951)
                     /locus_tag="PLE88_01015"
     CDS             complement(19926..20951)
                     /locus_tag="PLE88_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOM75077.1"
                     /translation="MKKSAAKLEQLKSALEGHTTLLIILQDNPDPDAIAAAAALRRLA
                     NSLFNIQCSIAHGGRVGRGENRAMVKYLGLNLRPMSQIDIVQFELIALVDTQPGAGNN
                     SLPQGIEPDIVIDHHRCRPQTRVCRFTDIRSRYGATATIMTEYLQSAKIKPDVPLATA
                     LLYAIRSDTQDLGRDTTRADIAALTRLMPIANKRMLSQIQRGKVDRDYFQMLENALKN
                     AYVYSNAIVTTLGIIENPDMIAETADLLLRDDLTNWVMVYGFVNDKMLISLRTEQDEL
                     GADKVIHRMVARKGTGGGHPSYAGGQIPLGALSEVQKTRLVRKVIDRFLQQVTNELSK
                     PQPLLPR"
     gene            complement(20966..21565)
                     /locus_tag="PLE88_01020"
     CDS             complement(20966..21565)
                     /locus_tag="PLE88_01020"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOM75078.1"
                     /translation="MDISFVNSDIVTWIVIPVLIFLARIVDVTIGTARVIFVSRGYRL
                     LAAAAGFVEVLIWLLAIGQIMKNLSNPICYIAYASGFAVGNYLGITLAERLSLGFVLI
                     RVITNKNAAGLIESLKNSDCGVTSIQGSGVFGPVAIVFTIAPRHQIDQIIRIIHQFNP
                     QAFYSVEEIGRVSASRPFKPSGPVLGRFVNIFRPFHKGK"
     gene            complement(21591..22487)
                     /locus_tag="PLE88_01025"
     CDS             complement(21591..22487)
                     /locus_tag="PLE88_01025"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOM75079.1"
                     /translation="MIANQKDIIVVSDLHLGDGGPRDNFSADRKEYQFNLFLDFVENK
                     NAEIFILGDLFEFWQANVGKVLIRWLKLLDRLAKLQTIYVVGNHDSDFQDLIGTHLLA
                     HPFFERMSGPFERVIGGKRFKFMHGHELDPFTRSGTPRWGRMMSILAGIVEDRKGSPL
                     LSAGGFTEKTLLKLARAFMWMMNNSVNLFEKSDLHEKPHALAEDLTPAQDPGKIKGIL
                     ALYHKNKIQEGYHYLIAGHTHAVGRFQDWYYNCGCWVGLRTHFLEILHDGQIHVCEWK
                     RDHPIVMEKVNLCVEETESSPF"
     gene            22675..22983
                     /locus_tag="PLE88_01030"
     CDS             22675..22983
                     /locus_tag="PLE88_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOM75080.1"
                     /translation="MVAGLLTVFIHLDGIDSLKDKRRIIKSTVERLRSRFNISVAEIA
                     AQDSKQLAVIGIACVSNEGPYLNEQLDKIIGFIVNDGRFFVGQIHRETFPSNYELPRL
                     "
     gene            23051..23731
                     /locus_tag="PLE88_01035"
     CDS             23051..23731
                     /locus_tag="PLE88_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOM75081.1"
                     /translation="MNMTDNTWGVIFDVDGTMVNNTAYHRAAWFELCRRYGIPMDHKS
                     YHEKIHARSNDKIVPNLFGPSADQEFICRIENEKETIYRESFRPVMQENPGLSDLLQS
                     LVKRGIACGAASNSPKENVDFILDGLNLRSYFRAVTFRDMVKAGKPDPELFLRAAQLL
                     GIPPQRCLVFEDSASGFKAARAAGMRYIAITLGGDSHELAEAYDAAAICEDFTSVNAD
                     TLAKLFKQ"
     gene            complement(23811..24323)
                     /locus_tag="PLE88_01040"
     CDS             complement(23811..24323)
                     /locus_tag="PLE88_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM75082.1"
                     /translation="MTAVEQYIELRTQVDQITAKLALIHSARMACRKGCCQCCMDLSV
                     WPVEFYSIVEEMKAAGWPRPQLSEDKQCCFLDEQGACQIYPFRPLICRTHGLPLVYWH
                     DETDPAGWGVMFCKKNFKDAENIEFGPDNTLNMEVVNEKLARINLSFLGERKDLNIRP
                     EDRIELKRLF"
     gene            complement(24351..25478)
                     /locus_tag="PLE88_01045"
     CDS             complement(24351..25478)
                     /locus_tag="PLE88_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOM75083.1"
                     /translation="MPRPFTLLIKPAGPDCNLACDYCFYTCKQDFFGGSTHRMSSQIQ
                     QKLVKSYLECRFEQSSFAWQGGEPTLMGLDFYKRLVDLQKKYASPKQVIVNTLQTNGV
                     LLDDDWAKFLAENRFLTGISLDGPKEFHDYYRKDKGGKGTFDRVIAGIDACRRNKAEY
                     NILVLLNNINIQHPDELFDFFTGLGVEFLQFVPCVEQSKEAMNKPAPYSITPQQYGLF
                     LNRIFDRWLDYGVRRLSIRLFDSILSCLIGRPPTECTFGRRCADYIVVEHNGDAFCCD
                     FYVTSETRLGNIMEAQIDVLADSEIKRNFNRKKAEIDNKCLVCRYLDICRGGCPKDRA
                     MLSGTHKVPSYFCEGYKMFFDHALPHLRAMAKNIQQGYLPV"
     gene            complement(25492..26607)
                     /locus_tag="PLE88_01050"
     CDS             complement(25492..26607)
                     /locus_tag="PLE88_01050"
                     /inference="COORDINATES: protein motif:HMM:NF023695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HOM75084.1"
                     /translation="MKNKKLTGMSMFNVLYALAILLLASSTFAEETKFWGAADVSAAV
                     FLDDSHFVVANDESNHLRIYQIDRPQSPVEQINLDSFLQAEPDSPEADIEAAARVGDR
                     IFWITSHGRNKDGKLRPSRYRFFCTHIQSGQTGGERLVIEGKPCKTLVQQLLAHPSPV
                     QEILKQATQFDQPLSKKQREKLAPKEQGLNIEGLVYYPFDESLLIGLRNPLFKPKGQK
                     EKLAVAVQLLNPQDLAIAGSAARFGRVLLWNLDNRGIRGMEYNSADKTFYILAGPVDS
                     ETASALFRWEGDFEKQPTLVVEWAENSSAFTPEGIAVGPDGLLWLFSDDGMLEIPVES
                     PSQCMEGKLLPNGTCPNKFLSDDGRKTFRIQKINLNP"
     gene            26907..27335
                     /gene="rplM"
                     /locus_tag="PLE88_01055"
     CDS             26907..27335
                     /gene="rplM"
                     /locus_tag="PLE88_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOM75085.1"
                     /translation="MKSTLAKKESVQRNWRIVDADGAVLGRLASKVAMVLMGKDKPIY
                     TPHVDTGDFVIVVNAEKVRLTGQKADKKEYQTYSRYPGGQKIIPFKKMLQQHPERVIE
                     LAVRRMLPKSTLGAHMFKKLKVYRGPEHAHAAQQPQKMEL"
     gene            27363..27845
                     /gene="rpsI"
                     /locus_tag="PLE88_01060"
     CDS             27363..27845
                     /gene="rpsI"
                     /locus_tag="PLE88_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOM75086.1"
                     /translation="MTETKNDGLLINPNIVTNTAAQPSGTKAVPDKGGFVWGTGRRKS
                     SVARVRVKPGKGIVTINDRPVHDYFKQIKDQDAVWAPLKATNTLGGLDVFVNVTGGGT
                     TGQAGAVMLGIARALKNFDPNLLKPLRDGGYLTRDSRMVERKKPGKPGARRSFQFSKR
                     "
     gene            27936..28943
                     /gene="argC"
                     /locus_tag="PLE88_01065"
     CDS             27936..28943
                     /gene="argC"
                     /locus_tag="PLE88_01065"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOM75087.1"
                     /translation="MIRAAIIGAGGYTGVESIEILLRHPEAKVSYLTALPEECMHVAD
                     MFGQLRGRLDMMIEPLDLDKLASKADVALCCLPHKVSMSFVPKLLATGIKVIDFSADY
                     RIKNAAVYEKYYEPHTDKENLSHAVYGLPELFRSEIKGARLIANPGCYPTGASLALAP
                     LLKHGLIKTSGIIVSSVTGVSGGGKKPSPNFHFPYMNENIYPYGVGVHRHMPEMEQVA
                     STLAGKTVELLFQPHVGPFDRGILSSTYSDPVGTITADEVMTLYQDFYKGEPFVQVLS
                     KPPAVKHVAKTNYCHIFPTVAKGKIVVFSVIDNLIKGASGQAIQNMNILFGLPETMGL
                     E"
     gene            28940..29800
                     /locus_tag="PLE88_01070"
     CDS             28940..29800
                     /locus_tag="PLE88_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOM75088.1"
                     /translation="MNSVNSGAVKVATCQFPVSADIASNASFIRTLMRQAAEQGAEIV
                     HFSECALSGYAGVDFDSFEGYNWDLLAEKTHEIIALSGALHIWTVLGSTHRLTEPHKP
                     HNSLYLISPDGQLADRYDKRFCTENDLSYYTPGSHFSFFNINDVKCSLLICFDLRFPE
                     LYRALYKENVRCIFQSFYNARQKGPSIHSEIMRQTMQAHAAMNGFWVSMSNSCGWFCP
                     YPSCFIQPDGRIAAQMDEHREGLMINIVNLQENFYDPSALFRDMAIAGKLNNALEVID
                     DPRSTDRHHL"
     gene            complement(29808..30680)
                     /locus_tag="PLE88_01075"
     CDS             complement(29808..30680)
                     /locus_tag="PLE88_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75089.1"
                     /translation="MGPLSAAGSTKTYQVEEFIRVLTPVSFLCRLKGYEPAPSVRFQV
                     FLRGISPQNATDSDAQFFKEQLATARKIELRNPVFRNYFRVEADVWADGNDLSLRLIK
                     QNPSLAPTPEQAFSQNTQPSSLPRPASAISANLLPADVRYPAVRTRRVSLQTLLDTPV
                     DCSSLRSDTPFSEALDFLCGSVVPRLPLVVLWGDLQANALVEKDTPIGVEGFGRMRLG
                     QVLDSILRSVAVGQVKPVLAAEGGILTLGTRVGLQEKNKTRVYSIEDLLMAPSRADED
                     SQSGYGGQNRGNTR"
     gene            complement(30767..33748)
                     /locus_tag="PLE88_01080"
     CDS             complement(30767..33748)
                     /locus_tag="PLE88_01080"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOM75090.1"
                     /translation="MMREIPKIYDPSSVERQTAELWEKGQYFHADEQAASPKGSYSIV
                     IPPPNVTAALHLGHALNNTLQDILVRWRRMQGYNTLWMPGTDHAGIATQTVVEKRLLA
                     EQGKRRTDFRRDEFVSHVQSWKDEYEARILGQLKSMGCSCDWTRTRFTMDEVCAKAVR
                     TVFFNLFKDGLIYRGKRLVNWDPATQTVLADDEVEHQTIQGFFWYLKYPLQTPAIIDG
                     RKVEYVTVATTRPETMLGDTAVAMNPADPRATYLVGKIVRLPIVGRLIPIIADEHVVL
                     PNPDSPDEKAKFSTGFLKVTPAHDPDDWAIGQRHGLAVINVMAPDGSISEKYGWTDWE
                     DIKNPDVENVIGLDRFEAREAIVEWFRQENLLEDVREYSHEVGHSYRSHVPIEPYLSD
                     QWYIAVKKPIPNISCQLPEVKCETETRDGVTFIKGTDVPVNSLAGLALVPLLEGRLTF
                     YPQRYAKTYQTWLENLRDWPISRQLWWGHRIPVWSKEGNHKNPFPNAVFTAIVQNEQQ
                     SLTYACVKADCEEIEQALDAEGWLRDEDVLDTWFSSALWPFSTMGWPDENATLKTFYP
                     TDVLCTAREIITLWVSRMVMMGQYCVGDIPFSDVYIHAMIQDGFGRKMSKSLGNGIDP
                     LVAIDSHGADAMRFTLASMTTDTQDIRMPVTELELPDGRKVNSSPKFDLGRNFCNKLW
                     NASRFAMMNLEGVDINAFTASQMDITDRWILSRLARTVASVSRDLEAYKFNEPLNEVY
                     KFFWNDFCDWYLEWIKPRMQNPEQKPVAQNVLAFVLDQILRLLHPFIPFITEGIFQNL
                     NSIAPQRGLKGLMSLKPAQSIMIAQWPEYTAEWVDSISERKIETVQNIVRAIRDIRSR
                     YTIAPSKPLDCSAAAPMEVCQVLNDNRSLVQQLANVGQFQAQPDFKKPNNAAAAIVEE
                     VQLFVHNVVDPEAERQRLQKQKEFVEKGIKPLAAKLANENFVARAAPEVVQQARQKLR
                     ELTEQLEAIERHLAELQIKDS"
     gene            complement(33847..35355)
                     /gene="rho"
                     /locus_tag="PLE88_01085"
     CDS             complement(33847..35355)
                     /gene="rho"
                     /locus_tag="PLE88_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOM75091.1"
                     /translation="MAKTTAKTRKKRNPKDTATQPEAPEQSSQQTEIQPDQTVEEISQ
                     DISAEEKQLAASEAPAAEEPKTLTEEESTHAKYERIKRGNLHIADLQNLEVTDLHALA
                     KKEGITDYMGMTKQELIFRILKERIRQDGLMYGEGVLEILPDGFGFLRSPKYNYLASP
                     DDVYVSPSQIRRFGLRSGNIVQGQIRPPKESEKYFALLRVEAINYQEPDKLAEKVVFR
                     DLTPLHAEERLILETTPEELSMRIIDLITPIGKGQRGLIVAPPRTGKTVLLQKIANAI
                     SHNHPEVKLIVLLIDERPEEVTDMQRKTADDVEVISSTFDEPTSRHCQVAEMVIEKAK
                     RMVEYGEDVVILLDSITRLARAYNTEMPHSGKILTGGVDANAMQMPKRFFGAARNIEN
                     GGSLTIIATALIDTGSKMDEVIFEEFKATGNMELHLDRKLVERRTFPAIDISKSGTRR
                     EELLLDPREMELVYRLRKVLSEMNMVEAIELLKNRLAKTRSNAEFLMTLKLD"
     gene            complement(35631..36236)
                     /gene="coaE"
                     /locus_tag="PLE88_01090"
     CDS             complement(35631..36236)
                     /gene="coaE"
                     /locus_tag="PLE88_01090"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOM75092.1"
                     /translation="MTPPIIGILGGIASGKSTVAKHFERLGCRVIEADSIAHQVLNDP
                     EIIRDITDRYGREILNSSGLIERKKIAEQVFSNPDELGFLCRIIHPRVLQQCQILLDS
                     FSTDPNVRGIVIDMPLLLEVGWDKKCDFLIFVECDEEKKLQRVCKNGKIDLLQIKKRE
                     KFQISLDKKRQMAHYRIINNSDESELAEQVVKIFSTITGNK"
     gene            complement(36337..38124)
                     /locus_tag="PLE88_01095"
     CDS             complement(36337..38124)
                     /locus_tag="PLE88_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75093.1"
                     /translation="MVQKPTVVHVTHEATGKIGGIGAVLQGMFTSPAYNEAVGRTILI
                     SPLFSTEGNVFSRLGPGGEVLYSSLDGLIRSSYVSSFRRIEDAFNVEIVYGRRRFTDP
                     MNGIAGQPEILLIDVTRMEKSPVDQLKGAMFREFGIRSDRYEHYWDYEQWVRLALPAI
                     EALKAIGAAEPDKPTIIVAHEFMGMPTALAAVLDPFDFKTVFYAHEVAPMRKIVEEHP
                     GHDTMFYNVIKKAHQRDLYVNEVFGDQSSFFKYALVNAARYCDNILAVGNCVVDELRF
                     LAPEFATADIDLTYNGIPAYQITVAEKHESRDKLRQYCQNLLGWKPDFVFTHVTRLVL
                     SKGLWRDLRVLEHMDRELNALNRTAVLLVLSTETHRRREQDIYQMEAAYKWPVAHREG
                     MPDLSGGEAAFYVGVQEFNAKSRNIKVIFINQFGFDQASCGYRMPADMQFMDIRKGSD
                     VEFGQSIYEPFGIAQLEPLTFGGICVVTNVCGCAGFVKAVAGSEMVPNVIFADYTELN
                     GDAGMDVEEMLRIDRLYRDRIEHKISEKVALEICARLPKNDEELNHLLQSGYELARHM
                     GWESVVQNYVLKSLNKALRKKHTMQAVVV"
     gene            complement(38466..39338)
                     /locus_tag="PLE88_01100"
     CDS             complement(38466..39338)
                     /locus_tag="PLE88_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOM75094.1"
                     /translation="METITVIPKLSPRPADGHKGTFGKVLIVGGSIGYSGAPALAGIA
                     ALRSGAGLVRVAVPASVQPIVAAVNPCYTTIALKQDSNGRMSAQDAAVINQMLAENDV
                     IAFGPGVGMGAGVKETLLCLIKAEGLKLVIDADGLNVLSQCGGSGGWLEKKKADVILT
                     PHPGEMQRLWKSVFREPMPENRQTCASEFARKSKSIVVLKGAGTTVADGDRLYVNDTG
                     NPGMATAGSGDVLTGVIAALAGQGLSLFDAAVLGVYIHGLAGDAAAKIRGQVSLIASD
                     IVEHLGHAFIQVSE"
     gene            complement(39360..40262)
                     /locus_tag="PLE88_01105"
     CDS             complement(39360..40262)
                     /locus_tag="PLE88_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOM75095.1"
                     /translation="MFSVYCKFLNMINLKQISVIGMGLLGASVTLAVKRELPSAQVIG
                     YSHRAKTRQKAREAGVAHEIAETLQESVKNADIVILATPIQTFKNYFLEISRYLKDGC
                     IVTDVGSTKLLPHHWAKKYLPKKVSYVGSHPIAGSEKRGVEFARDDLLTNARCILTKT
                     NGTSTTSIELLRWFWSRLGCTVIVMSPENHDRILGRVSHLPHLTAAALVNANTLQDMK
                     FAGRGFIDTTRVASGPADIWTDILMTNPANCVAGIERLITQLRLIQDAVRSEDKKKIE
                     KFLTQAARKRAQLIQYKIEQKELF"
     gene            40441..41553
                     /locus_tag="PLE88_01110"
     CDS             40441..41553
                     /locus_tag="PLE88_01110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOM75096.1"
                     /translation="MKKEDTIDTINIVKQYLPFEEEIAAIDRQIEMLKQQNGQNSLQG
                     KNGYQIRALQVQQTEVLKKIYSSLTPWQTVQVSRHPQRPILSDYLNRMFKDVRELHGD
                     RCFGDDRAIITALGQIGHQKVMIVGQNKGRDIKEKVACNFGCPNPEGYRKALAKMKLA
                     EKYGLPIVTLIDTPGAYPGVGAEERGQAQAIAVNLMEMARLRTPIVCVIIGEGGSGGA
                     LGIGVGDKIAILEYAYYSVISPEGCAAILWHDGTKAPQAAEALKLTAKDLLKLGLVDS
                     IIPEPLGGAHRNIHDTIYNVEQFMIRTLAQLKRFSIDALLENRYKKLRQIGADCVKIS
                     SLTSATDTKRTGSDLALLKEKLDKLSSEQISASFGV"
     gene            complement(41589..42059)
                     /gene="rpiB"
                     /locus_tag="PLE88_01115"
     CDS             complement(41589..42059)
                     /gene="rpiB"
                     /locus_tag="PLE88_01115"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOM75097.1"
                     /translation="MKVALANDHRGINAIEQIKAIISQLGFECIDYSINADQPVDYPD
                     MAYAASTAVAQKKADRAILVCGTGIGMSIAANKVKGIRAALCYDELAAKLSREHNDAN
                     VLCLSGDLIGPTMMRKIVETWLTTEFVAGRHLRRVRKIQAIEEGMDPRQLNTEA"
     gene            complement(42056..43177)
                     /locus_tag="PLE88_01120"
     CDS             complement(42056..43177)
                     /locus_tag="PLE88_01120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOM75098.1"
                     /translation="METKILKIYDPKDETVQEAAQLLDQGALVAFPTETVYGVGCRVE
                     LSTIQRLNQLKGRPADKRYTLHVGNYEQLSKYVPVMNAQARKLVSRILPGPVTIVFEC
                     NEKGLKHITQTLPQEVIDLLYSDGTVGIRYPAHSIACAILSSSQFPVVAPSANLSGQN
                     PAINAEQVLTYFNGKIECIVDTPDERICYNKSSTVVKVGKSGIHILRSGAVPDSVIRD
                     MATLRILFVCTGNTCRSPMAEGICRKYFADILGCSVDELSRLGYIINSAGVVAFDGAP
                     ASRYAVEVCRSYKIDLTGHHSRLLTRNLAEQSDVIYVMSPHHRDEVVRLCPSADSRCF
                     LLDKRGMPIEDPVGLDREDYEACFRQIQTAIAERTDELL"
     gene            complement(43216..46209)
                     /locus_tag="PLE88_01125"
     CDS             complement(43216..46209)
                     /locus_tag="PLE88_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75099.1"
                     /translation="MRKISIGLISLGAVALAFWLYVRTMETSVPLPPKAADANVLTLP
                     QASDAGQTSQTVIHGVQQSRYVLLDPQTKQVRRVLGFEKLLNPQAESSRWLVEKPYLI
                     FYEMDYECRLESDSGVFQVETAGPNAAPKDVRDAQLNGNVVIRVTPRAGSSISQTFVY
                     MDDLTFSSERSEFATDGPVRVCSDQIDLKGFGLVLILDVAKGRIDYLHILDLEQLRLI
                     NMAASSASASEGSSSAAAPKNAASDTFSSAVRSPKSSTSKSMETSSQQPDYYQCILDN
                     NVVIHYGSQLVVAGADRVAVQNILLSRIDSTDKKAAGQKEGPVKSADSLTDGQKSPGI
                     SLTAPSSADSKNRDIVVLCDGGIILKPMNGADNSSMSEAAPELSIEMNGSPLRIERPS
                     SETGVVNEMLVSCGILKYKPAEDILRLFTNISQPEIVLGDPSLQGRIQTRGNVLWNRK
                     TQQASIGGPGKAFLSSKDSAQEPSEVAFGGKMELRFAEPETARTSVTVQTVNLTGGMN
                     AIFRQNGWYKTAADSAVFEFGEKSDIESAHLEGGVQFESLEPGRPQRAAAKTATLKFG
                     PGNSLTAAYMNGQVRFESLQDSSQSRATAETAVFYFEKDKVQRANLQGDVCVASQTGQ
                     LTSSDATIEFSADTSGAVQPAAVHASGNTVLQTKTASPEEPPARFEARRIDYDLQTGS
                     GLAHGPLQFTFYQAAKNDNQALQPWIPITVTAEKNAEFTADTSGSIRQVVFNQNVVAV
                     RRLEALNYIQTDTFHGEKLSVFPQKNKTGSMDIQRINITEGNVFAESLRMHEDVKLSH
                     VKLTCTDITWDRPENLLAAQGPGKIELITSDETPADMPEQTGINFRRPCVAQLVGFDK
                     IRWLLNDQKIVADANQETMQLVYIPLLEKKPERFIYVNSIQFNLDFAADSAGRTMLAK
                     VFTNQGIVYRENNADQTKTLHEMIGQTLDYDAQAGGGWLTINGTEANPCRLDGARVPA
                     VRYNLATGQIETSLSTIPGVLKTGN"
     gene            complement(46252..46770)
                     /locus_tag="PLE88_01130"
     CDS             complement(46252..46770)
                     /locus_tag="PLE88_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOM75100.1"
                     /translation="MAADNLAQIKLLILDVDGVLTDGGIFLNDQGIESKRFNVMDGHR
                     IKMWHRAGGLTAIISGRDTPSTIHRARQLDIAYVMQGCLEKLPAFEQLLQQTALAPSQ
                     TAYIGDDIMDIPVVRRVGFGVAVANAAEELKQLADYITVKQGGHGAVGEVVEYLLKAA
                     GKWDSLMERYRI"
     gene            complement(46775..47764)
                     /locus_tag="PLE88_01135"
     CDS             complement(46775..47764)
                     /locus_tag="PLE88_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOM75101.1"
                     /translation="MAETAIDIDFAKNVIESEGQAVLGLLKAIDWSAFRQVVQMFLEC
                     QGSVIVSGMGKAGIVGSKISATLASTGTASHFLHPAEAVHGDLGRIQSNDIVLALSHS
                     GESEEIVRLIEPLKQRQIKLVSIVGDPQSRLAVHSDVALCMGMLTEACPFGAAPSVST
                     TCMLALGDALALTTMKSRQFSIEDYARFHPAGALGRKLLTVGQAMWFKPDEPLPLAEM
                     DDTIETMLIKNEPLKRRGAVMVVDRQGRLAGIITDGDLRRAMAKMKEKTFAMVCRQIM
                     TPNCKCVRQDTLAAEAMAIFHKYRIDELPVVNSENKPVGLIDVQDIVTIKIVG"
     gene            complement(47803..49323)
                     /locus_tag="PLE88_01140"
     CDS             complement(47803..49323)
                     /locus_tag="PLE88_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOM75102.1"
                     /translation="MSREMLINITEGEECRVAVVENGSLEELYTERSNMVSHVGNIYK
                     GRVVNIEPAIQAAFIDFGTSKNGFLHISDLHPRYFGQKESEENIGRRKALAQRPPIQR
                     CLKKGQEIIVQVTKEGINTKGPTLSSYISLPGKYLVLMPWMTSVGVSQKIEDEQERKK
                     LRDMMEGIPLPKNAGLIIRTAAQLASKRELQNDIAYLKRLWGVISKRIDTEKAPAEIY
                     QESDLALRTVRDIFDSSIDTILCDNEAETRKIRDFLSLVQPRLKHRVSFYEGDTPLFH
                     KYNIEKEIEKIQSTRVELKSGGSLVIEQTEALVAIDVNSGRYRKQTNAEQTALKINLE
                     ATREIVRQLKLRDLGGLIICDFIDMRDSRNRHEVEKEFRNALKSDRARSKVLKMSAFG
                     LIELTRQRMRPSLQSSTYLKCPHCSGTGVIKSIESQSIEVLRIIRMAAMKKEIHRIEA
                     TVSPEAADFLLNQRRAVLAQIEAENGKEIRIHPDTSGTLGQARIECFDERGSVVKF"
     gene            complement(49344..50006)
                     /locus_tag="PLE88_01145"
     CDS             complement(49344..50006)
                     /locus_tag="PLE88_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOM75103.1"
                     /translation="MSEQVYTLIADFSITGNAAYLSHQETLTLFERAMIRAEVPLIFS
                     SGFNPRPHLSIPLPRSVGVQSAAERLCAAVRFSKGIIPQDLSGGIQRQLPVGCTIRQI
                     QCREGKITFQPRSVRYAFFVEPPAETALMQHCQQCRQSIQDKRPIEVQRLNAKTGHFV
                     KLDISPFVEKMDIFADRIELVCRVSPAGTVRVDEMMQWLHLDQPMLREPVLRMEVQWE
                     QN"
     gene            complement(50108..51844)
                     /gene="ptsP"
                     /locus_tag="PLE88_01150"
     CDS             complement(50108..51844)
                     /gene="ptsP"
                     /locus_tag="PLE88_01150"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOM75104.1"
                     /translation="MEIRKGIAVSPGIAIAKPMLIDSKDYRIPRRSILPSQRTHEIQR
                     LRKAFATAVAELEQVKSAKNIEEDKIRDIFAVHLRFLRDRSFRRRITDMINHELVTAE
                     YAVSAILREIASHFAGIDDPYISERAADIYDIEKRLLVHLLGKRQEELDHLREEVVIL
                     ARDLTPTQTAGFNKTFIKGFATEKGGRTSHTAIVARSLGIPAVVALGDLSAEIPPDCV
                     VIVDGNRGVVVIDPDEETRRQYDRYAQQITQFVSKLNELKDLPAQTKDGVEIQLLGNI
                     EFPDEAAVVISKGGQGVGLYRTEFLYLYGGQEPTEEDHYSAYADAIRAVEGRTVVIRT
                     MDLGADKLPHSGWHPNETNPVLGLRSIRYCLQALPLFKTQLRAILRASVLGDVRVMFP
                     LITNLQELRQAKMILRDVMEDLDEYAVPYNAQMPVGIMIETPSSALTAALLANECSFF
                     SIGTNDLIQYTLAVDRANEHVSTLYSAAEPAVLKLIRNVIQDAYKAGIGLSICGEVAS
                     DPEFIMLLIGMGVRTLSLAPPMIPEVKKIIRSVTMEECNELSRKVATMDSPRQIKNYL
                     RDAATRILPESF"
     gene            complement(51853..52161)
                     /locus_tag="PLE88_01155"
     CDS             complement(51853..52161)
                     /locus_tag="PLE88_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOM75105.1"
                     /translation="MSKPIHEIEVEILNPEGLHMRPAMQFVDLANSFDSDITVSHQDL
                     TVDAKSIMQMTMLAATCGSRLRIQAVGADARQAIEALKRLVEQKFAGDSPVEAGEKEG
                     "
     gene            complement(52211..52681)
                     /locus_tag="PLE88_01160"
     CDS             complement(52211..52681)
                     /locus_tag="PLE88_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM75106.1"
                     /translation="MKLADIVCLDAIVAQLESTERDKVIQELVASLSKHKKLQGADPK
                     MLAKAVIKRENEASTGIGKGVAVPHVKHESITEPVAVVGCSQNGIDFSSLDKQPVYSV
                     FLLLSPTNKPDKHLQAMETIFKNLQKDDFRRFLRQAQTRQEIIEAIRDADEDAG"
     gene            complement(52751..53101)
                     /gene="raiA"
                     /locus_tag="PLE88_01165"
     CDS             complement(52751..53101)
                     /gene="raiA"
                     /locus_tag="PLE88_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOM75107.1"
                     /translation="MLVTVTGKHIEITDTIRAHIQEKVDKMPRYYDGISRIEVIIEGS
                     EGGKQSVEVIAHAEHNNPLVAKEIGMDTYTCIDMAVHKLERQLRKIKEKQREHKAMPP
                     AGIKTLPLSEEDVV"
     gene            complement(53126..53830)
                     /locus_tag="PLE88_01170"
     CDS             complement(53126..53830)
                     /locus_tag="PLE88_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOM75108.1"
                     /translation="MQALKNFRSYFFRGVAALLPTILTIWIFVQCYIFIQDKISIHIN
                     RGLVRLILLFSDRWDYEFLTSFWVKGQGKITGFFIAIIGVCFVGAFLASVMGHTIWHY
                     IEKTLMKVPLIRQVYPYIKQVTDFLLTKKGLEFSSVVAFEYPRKGIWSIGLVTGRGLK
                     KVAQTVQAELLTVFVPTSPTPFTGYVIMVPKTDTVELDISIEEAIRFVVSAGVIAPAE
                     QRPNGAPCAEPVLENE"
     gene            complement(54087..55109)
                     /locus_tag="PLE88_01175"
     CDS             complement(54087..55109)
                     /locus_tag="PLE88_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOM75109.1"
                     /translation="MTTKENQKLKPRLLLLLRIVIAAVACYIIFKDVDFRQVGLTLKR
                     MHPLVLPAASLIYIFSQALLGLRWWIFLRAQGVAISLWLTIKLTFMGLFFNNFMPSSV
                     GGDLIRAWYVSQHSPKKLQAALGVIIDRIMGLVSTLIIAVAGYLLFMRGQKGMLQVSH
                     SNSLISSLLERYPHIRFYSVILVVLIVISVFVLGRWINFEVILKRLFERVLYFWGQLK
                     EVITIYYHYPLVLVVSFGLTLLLQSIVILSYWAIGRELGIGACWGYYFVFFPIVWIIG
                     SIPISIAGIGILEGGVVFLFVTFAHADLESVTALALCQRITWIAASLPGMIVHLLGPH
                     HVGNKQ"
     gene            55226..55636
                     /gene="hisI"
                     /locus_tag="PLE88_01180"
     CDS             55226..55636
                     /gene="hisI"
                     /locus_tag="PLE88_01180"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOM75110.1"
                     /translation="MSITNKEIEEGLSFKPKFDASGLITAISQDAQTGQILMVAYMNE
                     EALKLTLQTGNAVFYSRSRKKLWKKGEESGHVQKVRQILVDCDQDCLILKVTVDQGQC
                     HVGYQSCFYRQVVSDKTLEFISEKTYDPKQVYKK"
     gene            56094..56567
                     /locus_tag="PLE88_01185"
     CDS             56094..56567
                     /locus_tag="PLE88_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75111.1"
                     /translation="MLLYVLRFLFFITSTAFLLVGLNRTAKTDQDIYIFYAALILTVV
                     FIAVEWLTPKKALFTLTGVFFGLLVGMVINWAVNWIIMMMNDLFWQLPPNTLRMGTWF
                     ADLCICFLCISIVIRTKDDFRFLVPYVEFTRQTRGLRPLVLDTSVIIDGRIADIA"
     gene            56595..57197
                     /locus_tag="PLE88_01190"
     CDS             56595..57197
                     /locus_tag="PLE88_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOM75112.1"
                     /translation="MPRFVLNELQLIADSPDKIKRNRGRRGLDMLNKLQHSPVVEITV
                     DDTPPPGLDDKSEVDQKLVAFTKNCDGRLVTNDFNLNKVASLRGVDVININDLANALK
                     TVTLPGETMSVKILRVGEDPHQGVGYLEDGTMVVVEGANDKIGQTVTFAVTSALQTSA
                     GRMIFGKCDNAGGPATNENAGLERNGSYDSRKRRRSYPNT"
BASE COUNT        14793 a        14601 c        12707 g        15385 t
ORIGIN      
        1 acaattacgg tctgtcggcc gagacaatcg gcggccagta ctatggggtt accggcattc
       61 gtccgtgcat tgacccggcg ttcgcaggaa gtatctataa ccgtgacaat accggttcct
      121 cttactgcaa ggtggatttg gcagatttcg ccctgctggc ggccaactgg ttagccgatg
      181 gactttgggt tgctccgtga aaataacgaa aaaacagggc agggggcggt ctcctgtctg
      241 ttatacaggt tttggcggtc aaagcctgtg acgagtaaac attttgtatt ttggaggatg
      301 taaagatgaa aaggttaggt attttgttat tgctgggtat gataggtgct gctcaggctg
      361 tgctgattga tgacttcagc ggcgacctca gtgcttatac aagcactgta atcctcgatg
      421 ccaatggcgg cggtgccaat actgcggcat ggcaaatcag cggcggcacg cttcagctca
      481 ataccacggt gtacgatggg attgaacaat atgcgatgat ttacagcggc ctaacactgg
      541 gtatcgggca ggaactgcag gccgatgtcg tcattggcgc aactggttcg caggatatcg
      601 ggctgtatgt gggcggcaca accccgacaa ccggcgttcg tcgggactac atcgcgatgt
      661 atcgccgcag cagcggccag ctgttctcac gcggttttga tggaaccaca gaatatacat
      721 tagcaggaaa ttggaccaat aacattccga ttaccgcatt gtttattgcc cgcattgcag
      781 aaaataccta cgaggccggc tggtatgacg gggcaactcg taatgttctt gtcacccgaa
      841 ctccggctta tgccaatgat gctgatgtga ttggtttctg tgcagatgtc cgcggcgtgg
      901 ggattgtcgg cagtcttgat aacctccgca tcgtgccgga accggctaca ctgaccttgc
      961 tggggcttgg cactgggctt ttggcccgca agaggaaata agcaaacctc taaaactaat
     1021 cagggagatg ttcaccccga ggccggattc tgattaaagg atccggcctt aagggggatc
     1081 taaagatgta aatacagaca cagcagacaa aactattaaa aagtgcggcg ttggtataag
     1141 cattatttct ttgagtatca gttattccgt tgcagtgaaa gaaagatacg tataacgaac
     1201 ggctacaata acggggagta tatgcccatc cacacaaccg aattgaaata tattggaagg
     1261 catcttttta tcttttatct attcctctat tcggtatgct tggcttttcc aacggctgtc
     1321 aaaattcagg acaccgtagt tgatacacag gcaatgacct taggggcagg agccccctac
     1381 ggctatgcta ttaacggtct ttcgtttcag caagaggcac tggtaacgtt caatggctgg
     1441 cagtatgccg tttattataa cagttcgcga agagtctgtc tggcacgacg tcagctgccc
     1501 tcaggagcat ggtccgtcat cccctttacc gattatacac tcagcacaac gacggatgct
     1561 cataatgtcg ttacaatcgg aatctgtccc aatgacggca ccattcactt gtcgtttgac
     1621 catcatggcg gcagcctgca ttaccgggtt tcccaacccg gcgccgcatc ccatcccgat
     1681 tcggttgtct ggaatgcagc tttgttcggg cctgtgcgca gctatctgat tgccggtcaa
     1741 acaattacca gtgttacata tccgcgattt tggcagactc ccgacgggaa tctgcaattc
     1801 ggcttccggg ctggggcatc cggcaatggg gattggtata tggccgatta tacggcagat
     1861 gatggcttat ggagcaatgt ccgacaggta ataaaccgca gcggcaccta cacggattcg
     1921 ctgggaacaa gcaccacacg caatgcctat atgaatccgc tcgtatatgg gccagacggc
     1981 atcctgcacg ccacttggac ttggcgcgaa agcgccggcg gagccaatca tgatattgta
     2041 tacgcatgga gtcccgacgg tggcttccac tggtacaaca acaatgttgc cgccggcttt
     2101 cggatcggaa ccggcagcag cccattacag acgctgctgt ctgttacttg gataaatgag
     2161 gggcttcagc tcatcggtaa agctaccggc agcactacta cacagcaatt gataaccgtt
     2221 cattcttcca atgcggctgt catagctgtc gaccgatact acggaataat gaatcagcaa
     2281 gctcaagctg tagacgcaca gggccgcatt catacgataa tgtttcattg cactcccgaa
     2341 acatatgagg ggtattcata cagcacatgg gggcctcaag gtgcccggag gtattatcac
     2401 tattggcgca acgccaaggg gatttggacg cgcaacgagc tgccgggatg ggttggcagc
     2461 agacccaaat tgtttattcg ttccaacggc gatgcttatg tgatttacca gtcccgccgc
     2521 aatgtcaacc cgactgatac aggcatctac tttacagacg gcgatttgac tattcaggcg
     2581 gccaccgccg ccagttcttg gacagactgg cagattattc atatcgaacc gggctacttc
     2641 ttcagcgagg cccttgccga tccctaccgc tttcaagacg gcgtcttatc ggttgtcatg
     2701 caggagtctc cttcgacgat cgggggcgct tcgcggctgc ggatattgga ttttgaacta
     2761 aattgaaacc aggctccttt taaggcataa ataatgaaga acacagcttt ctatagtcta
     2821 attcttttgc tcccaatatg cgtacgggcc gctcaatttg acaattttga aagctatact
     2881 accggaaccg caggggcacc gtggatgcca acgcagggtt ccccccttat tgggaaagaa
     2941 gccgacggca accaatatat cgaaagcttt ggatcgtatt gtttgctggg agatcatgct
     3001 gtccccgcgc aagattcagc tacaaccgtt ttttacagaa tttacaagcc ggctggaacc
     3061 tccccggact gctctgttgg attaagcgat ttatctgctc caaccggtga ttggaatgat
     3121 ttcgaggtgt atgttgtcat tgtgggcggg gaactccgtg cccgcaacgg caccagcaac
     3181 acagcaatta taagtccaat gaactcagga gtctggtata atatatggct gattctcaat
     3241 aacagctcaa acacctacga tgtatacgta acaaacggat cggccagtgc aacagcttcg
     3301 gaccggaaag ccagcaattt cgtctttcgc aaagccagtt ctaatccgtt ggcggcattt
     3361 aagatctacg gcaggccgga atatggacct gtttgggtag atgatatcta tattacaaac
     3421 ggtattgatt tgacaaatcc agcagccgct gtatcccccc tgattataca caacccttcg
     3481 aatagttcta tcaagcaaag tgaggaggca atttttacaa ctgtctttgc gtgccagact
     3541 tctccttcga caatatggta taaagccgcc tcgccggccg atattccaat tgatacaagt
     3601 ctgccggcat tcgatctgca gcttgattat gatcccctta caagacacta tacatcaacg
     3661 ttgaaaatac tcgactgtac cgtttcaaat tcgggatatt attactgcaa gatttttgat
     3721 gaaagcggtt actctcggag cagtgcatct gctttattaa tcgtctacgg tctttcagct
     3781 cactggactc taaataaaga caggtatatt tccggttctt accttgatga actcgccggc
     3841 cttccagcct acgctgccgg agcttgttcc ttcacaaacg gtgccgacgg tacaccagat
     3901 ggtgcaggtc tgatatcccc ttctggggga tgggccgaaa gctctatttt tgatccagtt
     3961 gcccaatctg gacagatgac agtcagcttt tgggcaaaaa ccagcgattg gggaacatca
     4021 caagaggact tgctggccga gtctatcagt gatgaaatcc ttattgttcc aaatggactg
     4081 aaagccgacg ggcgatggca gcatataggc atcgtttttg atggtacaac cggcagactt
     4141 tatgtggatg ggctattaca ggcacagggg ctatggaaga tgccgcaaga caccacagca
     4201 gttatacgct tcggaacagc cggcacgggg cagagcatct ttagtggagc aattgacgat
     4261 attcgggtat ataattacgc tctgacagag tttgaaattg ctgatttgcg gtatgatttt
     4321 tctggaatca gttcctgtat tcttgatttt gatgcccatt atgattttgc cggccccgat
     4381 ggaacaagcg actgcattat agatattctg gatttagcag cctttgctgc tgcattcctc
     4441 gaagccgacc ctgcccgcga cttgaccggc ccggatggat tgcccgacgg cattgtggat
     4501 ctgcaggagt ttgcagagtt cgcatctatc tggctggctt gcggccttta tccagtttgc
     4561 aattaaatcc gtctgaatat cccaagcgtc cgccagccta tgccgtacga tggtccagaa
     4621 acccttccag tttccttgca cgcaccggat gctggagttt ccggatcgcc ttggcttcta
     4681 cctgccgcac cctctcgcgt gttaccttga aaatacggcc gacttcttcg agcgtatagg
     4741 tatagccgtc gccaatgccg tagcgcagct taataatctc acgctcacgg taggtaagcg
     4801 tcttaagaac ctcatcaata cgatccttaa gcatctcctg agcagccgac tgaaccggtg
     4861 attcggcaga gtcatcctca ataaaatcac cgaaatagct gtcctcactt tcgccgatcg
     4921 gacgatccag actgaacggg tgcttgctga ttttcagcac acgccgcgtt tccgcaatgc
     4981 tcattttggc cgcttctgct atttcctcaa tggtagcctc ccgcccaagc ttctggagaa
     5041 gctgtttgct gatggtccgc agccggctca tcgtttcgat catatgcact gggatacgaa
     5101 tggtccgtgc atggtcggca atagcccgtg taatggcctg acgaatccac catgtcgcat
     5161 aggtgctgaa tttatagccc ctgcgatact catacttatc caccgcccgc atcaggccgg
     5221 tgttgccttc ctgaatgata tccagaaaac tcaagccgcg gttgcggtat tttttggcaa
     5281 ttgaaacgac caaacgcaga tttccgccgg acaaacgccg tttagcatct tcatattgag
     5341 catagacagc ctcaattgcc cgcagacgct tatccagctg cttgggagtt tccagcacca
     5401 attcttccag accctgcagt tcctgcttca ttgcctcaat atcttccggt tcataatctc
     5461 cccccgggcc ggcttcaatc aaggcttgaa gctgatgcat tttctgctga ataccctgca
     5521 gttttttcat catcggctga atccgactgg ttcgaaggct cagctcctcc aaaagtgagg
     5581 caattttccg gcggttgcga tgaatctgct tcacattctg cttaatttcc tctatcgtac
     5641 ccgaccggga ctgaagaaaa agagccgaat ttcgttcgag aagcttttga attgtggtga
     5701 tattggcagg cagccgattt tttacgattg ttctctcctg cgtttcgccg ctgctcattt
     5761 tcattgtacg gtcaaacggc agacttccat catccacctg ctgaagaatc tctattgctg
     5821 ttttagcgca ataatcgctt tccagcacct tccgccggaa aaccaaacgg gttaactcta
     5881 ttttcttggc taagctgatt tcttcctcac gcgtcaatag gggaatttca cccatttgtg
     5941 ttaaatacat ccgtaccgga tcatcaatcc ttcgtccctc accctcagcc agctcctcct
     6001 caagaaccag atcttcttca atcccctctt cttcctcatc ggcgcctacc ttgtcgggtt
     6061 cttcaaaatc atcctcgacg cctttttcca catcggtttc atcaataagc tgaacgcctt
     6121 gctcatccag cgtcatcagc agactatcca gccgagccgg actgataaca tcatcaggca
     6181 gagcatcatt cagttcctca tacgtcaaaa atcctttttc tttgcctctg gcgacaatcg
     6241 ccttaatcac ctcctcattc ttctcgtttt gagaaagaga tgatacgtca tcgtcatcca
     6301 gaacggatat atcgatcgtt ttgtcagtgg tctctgttat cttatgtttt atgtccgaaa
     6361 tatccggatg aattgattcg tcagatccaa gcaattctgt gctttcaatg aatttcccat
     6421 ccgcaatatc ttcgattttc gcattatcac gatgatagac gatatctttt ttcccttttt
     6481 ttggagtcat agacttttcc ttgttcgtta taaaagaaat aaaaataagt ccttcccagc
     6541 attaaatgcc ggcttttcgc aaatcaggcc ccttgtgagc gatttgcttg agcatgtcct
     6601 ttagtacttc ttctctatcc aaataacctt ttttttcttc tattttgagc tgatttttct
     6661 ggtcttctaa taccttcagc gcttgttcga gccgcaggcg ggaatctcct ttgccgtgtc
     6721 ctgcatcatc catctcagca aggattacag ccgtttcaga cgattccaga cgagcgtaaa
     6781 gtcgggcaag cgaaggttcc aatccttcct cagccaaacc gaaaaaagct tctgcaatct
     6841 ctcggatggg gccggaggtg ccaaaatcat caagacataa ccgctcttta actgccggat
     6901 aatatgtcgg ctcctgaagc aatacctcca gtatttcacg ctgtgtccga atgattaaat
     6961 cctgccccaa ggattttgca gaagtttcag tttcgtattt acgctgtatc ctctgatttt
     7021 gtttctgaat tttatgaatt tcttcttcaa ttcggcctac aggaatttgc aataatgcac
     7081 tcaaacgagc cagcacaacc gccctggtta ccgaatccat ctgccgagca ctgaaggcgg
     7141 atgcggctgt ctgaaggaac tggcgaatgg cctctgcctt gtcagctaaa ttctgcccgg
     7201 cgtttaattg ctcttccagt aactcccatt taaactcaag cacatccact gcattatcga
     7261 taacatgccg aaacgcatcc gcgccggctt cgagcagata gtcacagggg tctttgcctg
     7321 tcggtacaaa tccgagacgt agatccactt tttcggccaa gcaaatttcc agtgccctat
     7381 tcgctgccgc cctgccggcc gtatcactgt cgaaaatcag cacaattcgc ttgcagtaac
     7441 gtttcagcaa tttcgcatgg ctatccgtta agcttgttcc caatgccgct accacattac
     7501 aaataccaaa ctgatgagcc attataacat cagtatagcc ttctacgact acagctatcc
     7561 ccgtctgggt gatttcctgc cgtgcgtggt tcaatccata catggattga cttttttcaa
     7621 acaacaccgt agccggagaa ttcatatatt tggccgggtc accgccaaga gttcggcctc
     7681 cgaaaccaat cactcgattt gtaacatcca ctattggaaa catcagccga tttcgaaatt
     7741 tgtcataaca gcccccggct tctttctcta cggccagacc cgcttcaaca agatgcttac
     7801 atccgatttt tcttgcagcc gcggctgctg caagatcatc ccatgcatcg agggctaatc
     7861 caatctgcca tttttttatg gaggcctcat tgattttacg cccggtcaag tacgtccgcc
     7921 ccggaattcc cttttccgga tgatgcaggt tcgaaaccca gtgtgccatt gcccagttat
     7981 taagctttgc catcgagaca gcatccaaca gactgccaga agcccctaca ttccgctttc
     8041 gcaaaggttc tatgcgaata ccagctctct cggcaaggcg ttcgactgat tgcggaaaag
     8101 tcaaattttc cttcatctgg acaaatttca gaacatcccc ccctgctccg caggcgaaac
     8161 atttgaagat ttgtttagca gggctcacat atagacttgg tttgtggtct gcgtggaatg
     8221 ggcataaacc aactaattcc ttgcccctct tttccagact cagatgctcc gaaataacat
     8281 caacaatatc gttagcctgc tgaatccggc tgattaaact aaattcccat ttgccggcca
     8341 caaacactcc gtaaggggtc gccctttcaa agtctcttaa tcttcttata gttcaataaa
     8401 atttaatact cgttacgtgc tctgtgtgca aaattactaa taagctattt tctgagaatg
     8461 ttaaaaacat aataaagtat agaaaggaaa gtcaaaaagt caagtttagt atcagaaatg
     8521 gcattttaat atagaaaaac agcgttttcc taaacaatgg acacttgaac aaacgaaaca
     8581 tataatctac tagaattgtc tgcaactaca cataaaactc aattgcgaca tatgttctcc
     8641 atatcatcca tattcaattc atcaagccgg ttaacaatga tttgtgcttt gccattgagc
     8701 tgttctgcag gataagttgt tgtcacagca accggatgca tacctgccgc agttgcggct
     8761 tcaagacccc agtgggaatc ctcaataaca acacactcat atggattgat aatagtattg
     8821 gaattctggt tgaggcgttt taaggttaat atgtatccct cgggatgagg ttttccatac
     8881 cgcacatcat cagccgatac aataacctca aaggctttag caaaatcagc tccccgcagc
     8941 atcagttcaa tatcactccg caatgctccc gagcatatcg cacagcgtat cccagcgtcc
     9001 atcagacgtc tgacaaatgt ttccacgccg tctatgataa tcgattcttg acctgccagt
     9061 tcctcgaata ggatcttttt ttccttcatc agtcggttaa taaccgctgc atcccaaccc
     9121 attttataat cacggttaac agcttcgata ttttccaaat ccgaataacc cagatactta
     9181 gcccaatgca cttctttcgg cacatctaca ccgtaccggt taaacaccct attcagcgcc
     9241 ttataatgga gcagttcaga atcacagatt acaccatcaa aatcaaagat taccgctttc
     9301 agcatacgtt ttccttaaaa cttaaatccc ccgtatttta ctgccgcttt ttcaaaaaaa
     9361 tcccgttcct caaaagagtc gcatacatgg ctttctaaga tttaaaccga caaattcgtc
     9421 ccggattttc tacccgggca aaaggagaac atttaccgtg cttggatgcg tcttaatatt
     9481 tcatcaaatc gatctgaaaa atttcccggt ccttggccaa catccgtggg tgctgccgat
     9541 gtggagccgg ctctgttcca cggccagttg gctgccgttt cttcaaagat ataggaatca
     9601 ttgcaaaacg cggcgtggtt gtccgcataa aggactgaca aaccttgagg aatagaattg
     9661 atatctctgc tcttgcggtg cgggacaacc ctccaatcct gtatattgtc gaatatcagc
     9721 ggcttaaaac cggcaatttt ttcaattttt gaataaccgt aattccaata attgaatgag
     9781 gttctaacca attgactgct ggcacctcga ggatccgccg gcgtccgctt gccccattca
     9841 tagctgccct tgtcagcagg attgaaataa tcagcgggat ccccctgccc aatataaaat
     9901 tcatagtaat aaggaatcac ataatgacga gtggtacggg gctgagcggg acaataaaag
     9961 actttcggat cgccaatata atgctggtca tacaacacag ccagatgaaa cggcctgaag
    10021 ccgccgccgg gctttgtttg gttgggatga tagaccatat agctgttgta aggcagcggt
    10081 ataaacgacg gaggattggc aatagttgct gtactgattg tataaagcgg catgatctga
    10141 ttatcattgc tttgagcata caggtgaaca ccaagacctg cctgccgcat attgttcagg
    10201 caaatcgtac gcttgacata atctttcgct ttggctgccg caggaaggat aatggacagc
    10261 agcagtgcta taatagcaat cacaacaagc aattcaataa gcgtaaaact gcgtttattc
    10321 ataatcggag tcctattaca atagcacctg ccgtatattc tctgccgctt cattataaga
    10381 atttccagag aaaattgcaa taattatacc gcagcagtcc gaaatattgg aattgcgctt
    10441 ataacacaaa aagctctcta ttcgagagct tttcatttat gttctaagga attcagcaac
    10501 aaaaaccaaa tgttcctgct ttaagctttt tatatttgct ccggtacagg tcctgcaaga
    10561 acttcgtact gtttgaaagt agagcggtcg cccgacagca cctcctcagc cagcgattta
    10621 atcatcgccg cgctgcgcgt tacatagcgt cgggcaagcg cctctttccg ctggatcatc
    10681 gggatcacag catcactgcc gccggccgtg cgcaccagca tcttgacgct gctggacgcc
    10741 tgtccgcaaa gcaggtatcc attaatcaga gcaattgcca tatccaccaa ttgccgcccg
    10801 tataaatcca tatattccac gccctgttct ttggtgaatg ccacacattt aagcagcatc
    10861 tgacttccgt cagacagcaa ggccagtaaa tctgttacgc tgtcgacata agacctagcc
    10921 gacaaatccg ccaaatactt ttcgcaggcc ccgccgcaaa ccccccgcac agcggctaca
    10981 atctgaagct ggctagtacc ctcataaatt gttgtgatgc gtgcatcgcg tgcgtagcgt
    11041 tcacaggcat aatcccgcat atagccgctg ccgcccaata tctgaatggc atcatatgtt
    11101 atccgattgc acatttcaga gcagtaatat ttgctcattg gagtcagcat gccggccaga
    11161 cgcttgacag ttttcagctg ctgcttggcc tctttgacct tgtccttatc gctgccggct
    11221 tctgcaattt tgctccaccc aatttcatga tccaccaccc agcttgtttc ataaagcagc
    11281 gctctgccgg cttcgatttc catcttcatt cgaatcagca tatcccgaac agccggcagt
    11341 ttttcgatgg gcatcccgaa ttgctggcga ctggcggcat aatctcgtgc aacgcggtag
    11401 gctgcttctg caatgcccat ggactgtccc gctattccga tacgggcccc gttcatcagg
    11461 ctcattacat acgttaccag tccgcgctgc cgctcgccga taagctgaca gggagtattg
    11521 tcaaaataca gctcacatgt cggggaaccg tgaatcccca gtttgtcctc cagccggcgt
    11581 accttgactg taggtccgcg gtcgcacaca aacaagctca ggccaagccc gccgctgcga
    11641 tccggctccg agcgggcaag aaccagcagc acctcgccgc agccgttggt gataaaccgc
    11701 ttgacgccgt gcaggtacca ctgcccttca tcgtcctgaa acgcccgcag cttgcaggcc
    11761 tgtaaatccg atccggcatc cggctcggtc agcaccattg ctccagttac tttgccggcg
    11821 gcaaaatcat gcagataacg ctgcttgatt tcctcgtctg caaaatagtt gattgtctcc
    11881 gcaatgccct gcagaccgaa aatgttcatc agtgaagcat ctgcccgcga tacaacctca
    11941 atcgccatag aataaatcag gttcgggaaa ttcaatccgc cgaaacggtg cggcagcgta
    12001 aagcccatca catcggcttt tgccagtgca ttaagcgatt ccgcaatccc tttggcatag
    12061 ctgacagaac cgtcttcgtt gagggtggta ccttcgcggt cgatgctctc gctgcgcgga
    12121 gcaataaaat cagcactgag ctgccctacg gcacgaagca ccaagtcata attcgtaatt
    12181 gcttcttggg catctaccgg ggctgtatca tactgaccgg catatttaaa cccttcttcc
    12241 tgcagctcgg caatttctgc caaattgaga tgccggaaca aaaactgaat gtcatcatta
    12301 tccgtataaa aatttcccat cgtcactgtc ctttaattgg ggacttcctg tcttaactct
    12361 tgattatact ttttccttaa tggcttttat catcatcggt acaacctgat taaggtcgcc
    12421 gacaatttta taatgggcaa tattgaaaat cggggcatcc ggatcattgt taatggcaat
    12481 aatcttctga ctgtctgcca ttccggcctg atgctggatg gctccgctga tgccgatagc
    12541 aatatacaag ctggggcgaa cagttgtgcc ggtttggcct acctgatggt cgcggtcaat
    12601 aaatcctaaa tccaccgctg cgcgggtcgc tcccggcgct ccgcccagac aattggccag
    12661 atcccaaatc agacggaaat tgtccttgct gccgactcct gccccgccgg ctacgacaac
    12721 acgcgaggcc ttcagattaa ccttcttctg ctgcacatgt tcagaaagaa tttcaaccgc
    12781 caaatcggaa tgcgacaaat gaacagtttc tttgacaacc gctgccttgc gggtcccatc
    12841 cggtatcggc atcggcatta caccttcacg gacagtagcc atctgaggcc agcggtcgta
    12901 attgataatt gtcgcgatga tgttgccgcc aaaagccgga cggatctgca gaagcaagtt
    12961 ttcgaaaatc tggcctgttt tcttgatttc gtgatggcct atctgcaggt cggtgcagtc
    13021 ggccgtgagg ccggctttga ccgcgctggc tatgcgcgga gccaaatcgc gtccgcacgg
    13081 cgtagcgccg tacagcacta tctgaggttt gtatttgtga atcaatccat caatcacttt
    13141 ggcataactg ctggtttggt aatgttccag cagagggtgt tcggccagat ataccttgtc
    13201 agcaccataa tgccccagcg gctcgcaaag atggctaata cgatcaccca gcagaaccgc
    13261 cgccagcgga accttcaact gtgcggcaag ctggcgtccc ttgcttaaca gttccagaga
    13321 ggtatcttcc agatgtcctt tgtgctgttc ggcaaaaatc caaacttcac cttgtttgtt
    13381 gacttctatc atttgttctt ccttgcgtta atacaccccg aggggggatt taaaccgttt
    13441 tggtaaatat gtttatccaa tcgtgtgatc aacaatcagc tcgtgaatca ttgcatcaat
    13501 accgctctgg gtcggctcaa tggttttact ttccttggca gacaatacca cactttgaat
    13561 acgatggacc ttcgtcggcg agccatcccg accgcaccac tgcaaatctg ctttaagata
    13621 atccaaatcc cactggtcaa taagtaaacc ccgcgtttgc aaatcgcggc atacagcttc
    13681 tacatctacc gaagggttac tttgagctgc cgcttccact tcaatccggc actttgccca
    13741 tttgtacttc atcaattgct tagcccgcgg cactcggggt tcattggctg catcaataac
    13801 cgtcagcagt actggcagac ggcttttgac ctcctgccat ccgttgccga gattgcgttt
    13861 ggcaacaata gtttttccat ctaatgacaa cagcgcttca acataggtaa tctgcggcaa
    13921 atttaatttc tctgctacct gcggcccaac ttgggctgta tcaccgtcaa tggcctgtcg
    13981 gccacacaat acaatatcgg gatgaagcat tttaaccccg cagctcagga tgtagctggt
    14041 cgccaatgta tcggaggccg cgcagcgacg gtctgtaatc agcacggctc tatcggcacc
    14101 tcgatataaa ccttctctca gcacatctgc tgctgccggt aaacccattg tcattactgt
    14161 aactgttccg ccatattggt ccttaatctg gagagccagt tccagcgcat taaggtcttc
    14221 gggattgaaa atggccggca gcgctgcacg attgaccgta ccgtcttcgt tcatcgcctg
    14281 acctgtaatc cgttttgtat ccggcacctg ttttactaaa acaacgcaat tatatcccac
    14341 tgatatttac ctccatgaga ttactttctg ctttcggtca taataaagac cccgtagttt
    14401 agcccaaaaa ctcaataagt caaacaaaaa acaccatcct gcatatatac aggaattact
    14461 ggatatagta tgggtattgt atgaatttta taatttgtca gacagacaca atccaatagt
    14521 gtcccttaac tctgatagga tttagctgct caaatggcgt tcaatgaaat gggtgtctac
    14581 ctgattttta acaaagagat tatgcgagag aatttccaaa tgagcgggaa tagtcgtttt
    14641 aatgggttca atttgaaact cccgcagggc ccttttcatt gttgcaatgg cctcctgacg
    14701 cgtgggctga tggacaataa gttttccaat cattgagtcg taatagggag atactgttgc
    14761 cccctgataa atatgggtat cgacgcgaac tccaaatccc ccgggaattc taaatttttc
    14821 aatttttccg ggacaagggg cataaccgcg tgccggatct tccgcattga tgcggcattc
    14881 gattgcacag cccctgtgaa caatatcccg ctgccggagc tcaagcgttt gtccggctgc
    14941 aacttttatt tgaagcttta tcaggtcgca tcctgttaca agttcggtta ccggatgttc
    15001 aacttgtatt cgggtattaa cctcaatgaa ataaaagttt ttatctttat ctaagagaaa
    15061 ctctacggtt gcggcattgt gatatttggc ctctttaatc agccgcaggc ctgccttgca
    15121 aagctcttct cgtgtttttt catccaatac cggacaaggc gattcttcaa tcaatttctg
    15181 atggcgccgc tgaatcgtac agtcgcgttc atagaaatga acagcattgc cgctcttatc
    15241 agctatcagc tggacctcaa catgacgagg ttcctgaata aacttttcca gataaacagc
    15301 accgttttta aaggccgctt ctgcctccat tcgggcagca gaaaatcctt tgtgcaagct
    15361 gatatcatta tgggcaacgc gcattccgcg gccgccgccg ccggcggcgg cttttataat
    15421 gaccggatat ccgattttat tagccagttt aacagcttcg gcctcatccc gaacttcgcc
    15481 gtctgacccc gggacacagg gtacacgggc tttgcgggct aaatcgcggg ctgctacttt
    15541 atcgcctaaa agccgcattg cagcagcatc cggaccaatg aatgtaatcc cgcaatctgt
    15601 acaaacttca gcaaaatgag cattttcagc caaaaaacca taaccgggat gaattgcatc
    15661 cacatctgca atttctgcgg cactgataat cgctggaata ttcaggtagc ttttagctgg
    15721 tgcagctgga cctatacaaa tcgcctcatc agccactttt acatacgacg cttctctatc
    15781 cgcctcggaa tataccgcta ccgcctctat tccaagctcg tgacaggcgc gtatgatgcg
    15841 aagggcaatt tcgccgcgat tggcgactaa tattctcgaa aacataaaaa cctctgctga
    15901 taaatcagga tttcaggaag agtatggaat cttaaggtcg gactttaaat aatacctgac
    15961 catattcgac tgcttggccg tctttgcagc aacattcaac gacagtgccg gaaacttccg
    16021 ccttaatttc gttcatcacc ttcattgctt caataataca tactaccgta tccggattga
    16081 ccttgcctcc aaccttaaca tacggttcag aatcggggct gggtgcctga tagaaagtgc
    16141 ctacaatagg cgatgtaatt ttaagcagtg tggcatcttc cgcaggtgca ggcggaactg
    16201 acacaacaga tggcgctgga gccgccataa ccggctgtgc cggagccgga tgcaagacag
    16261 gggcctcagc accgggacgc tttaggtgaa ctttggaatc gtcatcgacg atctcgagtt
    16321 ctatcaaatc attttccttc attaaatcta taagttcttt tattttttta atgtccatac
    16381 tctttttatc cgacatcgta ttggcatcct tttctgtata tggcaaatcg acttttttga
    16441 gggatttagc cgctcagtat agtcgtatct tctaaaaatg caagaacatt tgctgaccta
    16501 agcatgttat tatctcatat tgtagacata gcaggtgctt tttattaaat ttgcttcaaa
    16561 ttcattgatt tttatacgtt ttagtcgata attttcgaat attttgtgag atattgtttt
    16621 tactcttaat gatgaaagaa gattttggat gtcaaaagtt aactttatag aacggctgaa
    16681 aagcggtatc gtcattggtg atggagctat gggaacaatg ctctatcagc atggcgtttt
    16741 cgtgaacact tgctttgatg agctgaatct gacaaaaccg gatttggtgg gacggattca
    16801 cagcgactat gttcgggcag gctgcgactt tattgaaacc aatagtttcg gggctaatgc
    16861 ttttaagtta agccgcttcg ggcttgttga agataccgta agaatcaata ccgccgctgt
    16921 cgagattgca aaaacagttg cgggagaagg agttcttgtt gccggttcga ttggccccag
    16981 cggcatcaat aatccagccc ctgataatga attacagcgg tcccttttgg aagctttttc
    17041 ggttcaggca caggcattgg cttccagcgg agctgatttt cttattttgg agacatttac
    17101 aagcacttct gaacttctga ttgccattga agccgcgtcc aaaaccggct tgccgataac
    17161 tgcccagctg acctgtacag aacaatttga aacctttttc ggtacaccta tcgaaaaagc
    17221 aatttcccat atttctgctc ataatgcggt aacagcagtc ggtttgaact gctctatcgg
    17281 tccttccgat atgctcgagg ccttgaagcg gctgcgtcag ataacagata agccgctttc
    17341 gattcaaccg aatgccggcc tgccccgacg gattgaaggc cgcactcttt atatgtgtac
    17401 tcccgaatat atggctgaat atgccaaacg cttcttcgaa aatggcgcac gaattattgg
    17461 cggctgctgc ggcacaacgc cggcccatat tgccgaaatc gtcaaagctg ttcggccgct
    17521 ggacaaggca gccgctgcca aaccggttcc gatacaaatt accataaaag actccaaacc
    17581 ggcggggatg aaaccaatac cgcttataga aaaaagcaga ttggggaaaa aacttgcagc
    17641 aggcaagcca attacctgca tcgaattgac gccgccgcgg gggacagata ctcaaaaagt
    17701 cattgaaaat gcaaaattat gcgctgcgta tggcatagat gccatcaata tccccgatgg
    17761 tcctcgagca agtgcgcggc tgtcgccgct tgtaacagct ttgaagatcg aacaggcaac
    17821 agacatagaa acgattctgc atatctgctg tcgggataag aatcttatcg gtttacagtc
    17881 agacctgttg ggaatccacg cgatgggatt acggaacgtt ctgctgataa ccggtgatcc
    17941 gccgaagctg ggcgagtatc cagatgctac aggtgttttt gatttggatt cggtggcttt
    18001 gacacgcgtg gcagccggtt tgaatcgggg gctcgatatc gcccaaaatg aactgccgca
    18061 gcaactgtct ttaactatcg gtgtaggagc caatccggct gcatccgaac tgcacagaga
    18121 aatagaccgt tttcgccaga aggctgccgc aggtgctgaa tacgccatta cccagccggt
    18181 atttgatacc gagatgtttt ttgcatttgc agcagcagtt gaagactgca gactgccgtt
    18241 tattgcggga atctggcctt ttacaagcta taaaaatgcc gagtttatgg ccaacgaagt
    18301 cccgggagta acagtgcctg atgcattgct caaacggatg gctaaagcca agacacgcga
    18361 agatggacgc cgaatcggca tcgaaattgc gcacgaaatg attgccgtat tagccccgca
    18421 cgtagcagga tttgccgtca gtgcaccatt gggcaatgtg aaaatcgcat tagccgcttt
    18481 ggggcgaatt tcgcttaatg aactataagg cctcttccgc tttgcattga gccataaaag
    18541 cggactacaa ctgatacaaa ttctgcgttt cgggctattg tctatttttc caacgctacc
    18601 aaatcgtcat aggtctggcg tttgcggatg acggaaaagg tatcgccatc aacgagaacc
    18661 tcagcaggca acagacgggc attgtagttg ctggccatac tgaatccata cgcacccgcg
    18721 gtgaaaattg caatcaaatc gcctcgcttt acaggcggca aagccctgtc tttggcaaaa
    18781 tagtcggtgc tttcgcagat gggaccgaca atgtctacaa tttctgtgcc gggcatttcc
    18841 atgtctttgc tgcgcttttc gggaacaaat ttatcatcca cctgtaccgg ccagataaaa
    18901 tgaaatgcct catataaaca cggccggata agatcgttca ttccagcatc gacaataaca
    18961 aaagtcttct ggccgccggt tttcttgtaa agtgtttgcg tcaggagaat agccgcatta
    19021 gcgataatgc ttttgccggg ctcaagaatc agcttcaaac tcttttcctt cagcagcggc
    19081 acaatacctg atgcataatc cgctgctgac ggcacagtat cggtttcata atctgcgccg
    19141 tagccgccgc ccaaatcgag ggtatcaatt ttatagcctt tcgctcgcaa aatatctatt
    19201 aaaggaagaa tctttttgac gccgctgacg taagggtcta tcgttttgcc gccggaacca
    19261 agatggacat ggacggcaga caactcaaca acgccgttgc cggcatattg gtcaaaaacc
    19321 ttctgcgccc gctcaatatc cacaccaaac tttgtttctt tgacccctgt agttatatgc
    19381 cgatgagaat catatctaat atcaggattg atcctaagtg ctgccttagt ctttttgccg
    19441 cactgtctgc aaagctgaat cagattttcc agctcggctt cggattctat attgaaataa
    19501 gcaatgccgg catcaagggc ttccagaatt tctttatctg tcttgccgac tcctgcaaag
    19561 acaacctttt tcatatccgc accggcctgc acagcacggt acaattcgcc gccgctgacg
    19621 atgtcaaagc cgctgccggc ctgtgccata agacgcagaa tattgatatt gccgcaggcc
    19681 ttgatagaat agcagaccgt cgtatcaatc ccggcataag cggtctgaat tttccgcaag
    19741 tggtctaaaa tagtggcttt gctgtaaata tatacgggcg taccaacctg ctgggcaatt
    19801 tttgttacat ctacgccttc gacaaacagt tttccttttt tatagataaa tgaatccata
    19861 atgtcctttc tatacagtta tttcataaat aaaaatcttt tttatctaaa tttccagaaa
    19921 aatttctatc tcggaagaag gggctgcggt ttggataatt catttgtgac ttgctgcaga
    19981 aatcggtcaa taacttttct gactagtcgt gttttttgca cttcactaag agccccgaga
    20041 ggaatctgac ctccggcata gcttggatga ccgccgccgg tacctttgcg ggcaaccatc
    20101 cggtgtatca ctttatcagc acccagctca tcctgctccg ttcgaagtga aatcagcatc
    20161 ttgtcattta caaaaccgta aaccatcacc caattggtta gatcatcccg caagagcaaa
    20221 tccgccgttt cggcaatcat gtcgggattt tcaataatcc ccagcgtcgt aacaatggca
    20281 ttactatata catacgcatt ttttaaggca ttttcaagca tttggaaata atcgcgatcc
    20341 accttgcccc gctgaatctg actgagcatc cgtttattgg caataggcat taaccttgtt
    20401 aaagctgcaa tatcagcgcg ggtagtatca cgccccaaat cttgcgtgtc agaacgaatc
    20461 gcatacagca gtgcagtagc cagcggaaca tccggtttta ttttggccga ctgcaggtac
    20521 tcggtcatga ttgtagcggt tgccccatat cggctgcgaa tatcagtaaa tcggcagacc
    20581 cttgtctgcg gcctgcaccg gtggtggtcg atgacaatat cgggctctat accctgcggc
    20641 aaagaattgt tgccggcacc gggctgggta tctacaaggg caattaactc aaactgaaca
    20701 atatcaatct gactcatagg ccgcaaatta agccccaagt attttaccat tgcccgattt
    20761 tccccgcgcc cgactctgcc gccatgcgca atcgaacact ggatattgaa aagcgaattg
    20821 gccagcctgc gaagagcggc ggcggcggcg attgcatcgg ggtccggatt gtcctgcaaa
    20881 atgataagca gcgtcgtatg gccttcaaga gcagatttca gctgttccag tttggcggct
    20941 gattttttca tcacacaatc tccgtttatt tgcctttgtg gaagggacga aaaatattta
    21001 cgaatctgcc cagcacagga ccggatggct tgaatggacg acttgccgag acacgcccga
    21061 tttcttcaac agaataaaat gcctgcggat taaattggtg aataatccgg ataatctggt
    21121 ctatctgatg acgcggggca atggtaaaga caatggccac aggcccgaaa acccccgacc
    21181 cctgtatact ggtaacgccg caatcgctgt ttttaagtga ttctatcaat cctgcggcat
    21241 tcttgttggt tataacccga ataagaacaa atcccagcga caaccgctca gccaatgtta
    21301 tgcctaaata attgccaacc gcaaaacctg acgcgtaagc aatgtagcaa atcggattag
    21361 acaggttttt cataatctgc ccgattgcca gcagccagat gagcacctcc acaaaacccg
    21421 cagcagcagc cagaagacgg tatccgcgtg agacaaatat cacacgggct gtgccgatag
    21481 ttacatctac aatacgagcc agaaaaatca gcacagggat aactatccaa gtaacaatat
    21541 ccgagttgac gaagctaata tccaaactgc attctcctgt taagacgttg ttagaaaggg
    21601 gaactttccg tctcttccac gcacagattt actttttcca ttacgatcgg gtgatccctc
    21661 ttccactcgc agacatgtat ttgtccatca tgaagaattt cgagaaaatg tgttcgcagt
    21721 cctacccagc agccgcaatt atagtaccaa tcttgaaatc ggccaacagc atgcgtatga
    21781 ccggcaatca gataatgata cccttcctgt attttattct tgtgataaag agccaaaatg
    21841 cccttaattt ttcccggatc ctgagcaggc gttaaatctt ccgccaatgc atgcggcttt
    21901 tcatgcagat cgctcttttc gaaaagattg actgaattat tcatcatcca cataaatgcg
    21961 cgggctaatt taaggagtgt tttttctgta aatcctcctg ccgataacag aggcgagccc
    22021 ttccggtctt caacaatccc tgccaaaatt gacatcattc tcccccaccg aggcgtcccg
    22081 ctgcgggtaa atggatcgag ttcatgacca tgcataaatt tgaaacgttt ccccccgata
    22141 acacgctcga aaggaccgct catccgctcg aaaaaaggat gagcgagcag atgtgtacca
    22201 ataagatcct gaaaatccga atcatggttg cctactacgt aaatggtttg gagcttggca
    22261 agccgatcaa gcagcttcag ccatcgtata agaactttgc cgacattggc ctgccaaaac
    22321 tcaaagagat cccccagaat aaaaatttct gcatttttat tttcgacaaa atcgagaaaa
    22381 agattgaatt ggtactcttt acggtcagcc gaaaaattat ctcgagggcc cccatcacct
    22441 aaatgcaaat cactgacaac aataatatcc ttttggtttg ctatcaaatt gttcctttct
    22501 ttactgtgtt tttacctata atatctgttc tattttttaa tgtaaagagg caggattttg
    22561 actgatagga tctatttatg atagaataac atctgttttc ccaagaaatt ctctgtgttt
    22621 gaaccgaaat ttacacaata attatatgct cacaaataaa aggataaaac tggtatggta
    22681 gcaggactgc ttactgtttt tattcattta gacggaattg actcactcaa ggacaagcga
    22741 agaatcatca aaagtactgt tgagcgtctt cgcagtcgtt tcaacatttc tgtcgctgaa
    22801 atcgctgctc aggacagcaa gcagctggcg gtcatcggaa ttgcctgtgt ttccaacgaa
    22861 ggcccctact tgaatgaaca actcgacaaa attatcggat ttatcgtcaa tgatggacgt
    22921 ttttttgttg gccagattca ccgtgaaaca tttccatcca attatgaatt accccgcctt
    22981 taagcagcca gcttgacatt cgccgttttt tgtgtattgt agccggcttt gcttacaaaa
    23041 ggaatttata atgaacatga cggataatac atggggggta attttcgatg tagacggcac
    23101 gatggtcaac aatactgcgt atcatcgcgc cgcatggttt gaactatgcc ggcggtacgg
    23161 cataccgatg gaccacaaat cctaccatga aaaaatacat gctcggagta atgacaaaat
    23221 tgtgcccaat ctgttcggcc cgagtgcaga tcaagagttt atttgcagaa tcgaaaacga
    23281 aaaagaaaca atttatcggg aatcatttcg gccagtaatg caggaaaatc cggggctgtc
    23341 tgacctgctg cagtcattgg tgaagcgggg aattgcctgt ggggcggcat ccaattcacc
    23401 aaaagaaaat gttgatttta ttttggatgg attgaatttg cggagttatt ttagggctgt
    23461 aacttttcgt gatatggtta aagcgggcaa acctgatccg gaactctttt tgagagctgc
    23521 tcagctgctt ggtattccgc cccagcggtg cctggttttt gaggattctg cttcaggctt
    23581 caaagccgct cgcgccgccg gcatgcgcta tatcgctatc acgcttggcg gcgattcaca
    23641 cgaactagct gaagcatatg atgctgcagc catttgtgaa gattttacca gcgttaatgc
    23701 cgatacatta gcaaagcttt tcaaacaatg aggaaaaaaa catcggatag gtcaatttca
    23761 tgaaatgtgt cttcttcaaa cagataccga ttgacttctc ttttaatccc tcaaaaaagc
    23821 cttttcagct ctatcctgtc ttctggtctt atgttcaaat cctttcgttc ccccagaaag
    23881 gataggttga tacgggcaag tttttcattg acaacctcca tattaagcgt attgtcagga
    23941 ccgaattcga tgttttcggc atctttgaaa ttctttttgc agaacatcac cccccagccc
    24001 gccggatcgg tttcgtcatg ccaatacacc agcggcaggc cgtgcgtgcg gcaaatcagc
    24061 ggtcgaaagg ggtaaatctg acatgccccc tgctcatcga gaaaacagca ttgtttatct
    24121 tcgctcaatt gcggtctcgg ccatccggct gctttcattt cttcgacaat cgaataaaac
    24181 tccaccggcc agaccgacaa atccatacag cactggcagc acccctttcg gcaggccatc
    24241 cgtgcggaat gaatcaaggc cagctttgct gtaatttgat caacctgtgt tcgaagttct
    24301 atatattgtt ctactgcagt catatttttt gattttttag gaagttaaaa tcaaaccggc
    24361 aggtatccct gctggatatt cttggccatt gcacgcaggt gcgggagggc atggtcaaaa
    24421 aacattttat agccctcgca gaagtagctg ggaaccttgt gcgtaccact cagcatcgcc
    24481 cggtctttgg ggcagccgcc ccggcagata tccagatagc ggcagacaag acatttattg
    24541 tctatctcgg cttttttgcg gttaaaattt cgtttgattt cgctgtcagc cagcacatca
    24601 atttgtgcct ccataatatt acccaagcgg gtttcactgg tcacatagaa atcgcagcag
    24661 aacgcatcgc cgttatgctc aactacaata tagtcggcac atcggcggcc aaaggtgcat
    24721 tcggtcggcg gcctcccaat aagacagctc agtatactgt caaacagccg tatactgagc
    24781 ctgcgaactc catagtccag ccagcgatca aaaatgcggt tcaaaaacaa accatattgc
    24841 tggggagtaa tactatacgg ggccggcttg ttcatcgctt cttttgattg ctccacacac
    24901 ggcacaaatt gaagaaattc aactccaagc cctgtaaaaa aatcaaataa ttcatcgggg
    24961 tgctggatat tgatgttatt aagcagaacc aaaatattgt attcggcttt attcctccgg
    25021 caggcatcaa tgcctgctat cacgcggtca aatgtccctt ttcccccttt atccttccgg
    25081 taataatcgt gaaattcctt tggaccgtcc aatgagattc ctgtcagaaa tctgttttcg
    25141 gccaaaaatt tcgcccaatc atcatccagc aaaacaccat tcgtctgaag cgtattgaca
    25201 atgacctgtt tcggggaggc gtattttttt tgtaagtcca caagccgctt ataaaaatcc
    25261 agccccatta gggtcggttc cccgccttgc caagcaaaac tgctctgctc aaaacggcat
    25321 tccagatagc ttttgaccag cttctgctgt atctggctgc tcattcgatg ggtactgccg
    25381 ccgaaaaaat cctgcttgca ggtataaaaa caatagtcac aagccaaatt gcagtcaggg
    25441 ccggccggct taatcagcag tgtaaatggg cgcggcatat tgattgcgga ttcagggatt
    25501 caggtttatt ttttggatac gaaacgtctt ccggccatca tcactaagaa acttgttggg
    25561 gcaggtgccg ttaggcagca gcttgccttc catacactga ctgggagatt caacaggtat
    25621 ctcaagcatg ccgtcatcgc tgaacagcca cagcagcccg tcaggtccca cggcaattcc
    25681 ttcgggcgta aaggcagaac tgttttcagc ccactcgacg acaagtgtcg gctgcttttc
    25741 aaaatcgcct tcccaacgaa acaatgcact ggcagtttca gaatctacag ggccggccag
    25801 aatgtaaaat gttttatccg ccgaattgta ttccatgcct cggatgccgc gattatccag
    25861 attccataac aaaacccggc caaatcgggc agcggaacct gcaattgcta aatcctgagg
    25921 attgagcagc tgaactgcta cagccaactt ttctttttgt cctttgggtt taaacagcgg
    25981 attgcgaaga cctatcagaa gactttcatc gaatggataa taaaccagac cttcgatatt
    26041 aaggccttgt tcctttggtg ccagtttttc ccgctgcttt ttggaaagcg gctgatcgaa
    26101 ttgtgtggct tgcttaagta tttcctgaac tggagatgga tgagccagaa gctgctgaac
    26161 cagcgtcttg caaggtttgc cttcgataac caggcgttca ccgccggttt ggccagactg
    26221 gatatgcgtg caaaaaaacc tgtaccggct gggtcgtagc ttgccgtctt tatttcgacc
    26281 atgagaagta atccaaaaga ttcgatctcc aacgcgggct gcagcttcaa tatcggcttc
    26341 cggagaatcc ggctctgcct gcagaaaaga atccagattt atctgttcca ccggactttg
    26401 cggccgatct atttgataaa tacgcagatg atttgattca tcattggcta cgacaaaatg
    26461 actatcatcc agaaaaacag cagcagaaac atccgcagca ccccagaatt ttgtttcctc
    26521 agcaaatgta ctgctggcca ataataatat tgccagtgca tataggacat taaacatcga
    26581 catacccgtc aattttttat ttttcatata taaaattata accttttcta ctacaaaatc
    26641 aaacagcaac aaaaataata tatttaggtc taatttggcg aatatttgta ataaattcca
    26701 tatttttaca gtcatttttt aattttttta gtaaaaatgg aattgacaga gacacagtgt
    26761 ccattataat aatttgcttt cattagatac cgtattatat cagtaagctg tttgggataa
    26821 taatattctg cgtacagata aatacggtat atcgagtcgg attatttgcc gatgcgggaa
    26881 aaactaaact tataaattgg tgcaatatga agagtacttt agcaaagaaa gaatctgtgc
    26941 agcgcaactg gcgcatcgtg gatgcagacg gggctgtatt agggcgtttg gcctccaagg
    27001 ttgccatggt tttaatgggt aaagacaagc cgatttacac gccgcatgta gataccggtg
    27061 atttcgtcat cgtggtcaat gcagaaaagg ttcgcttgac cggacagaaa gccgataaaa
    27121 aggaatacca gacctacagc cgttatccgg gcggtcaaaa gatcatccca ttcaaaaaaa
    27181 tgcttcagca gcatccggaa cgggtaattg agctggcagt tcggcgtatg cttcccaaga
    27241 gtacgcttgg tgcccatatg tttaagaaat taaaagtcta tcgcggccca gaacacgctc
    27301 atgctgctca gcagccgcag aaaatggaac tgtaatttat cagctgatta cggaaagcat
    27361 atatgactga aacaaaaaat gacggtcttc tgattaatcc gaacatcgtt actaacacag
    27421 ctgcacaacc cagcggcaca aaggccgttc ccgacaaagg cggttttgta tggggtactg
    27481 gccggcgtaa aagcagcgtt gcccgcgtac gggttaaacc gggcaagggc atcgttacca
    27541 ttaatgaccg gcctgttcac gattatttta agcaaatcaa agaccaggac gctgtgtggg
    27601 caccgcttaa agctaccaat acccttgggg ggctggatgt cttcgtgaac gttacaggcg
    27661 gcggtacaac gggacaggca ggagctgtga tgctgggaat cgcacgagca ctgaagaatt
    27721 ttgatcccaa cctgctcaaa ccgctgcgtg acggcggcta tctgacacgc gacagccgaa
    27781 tggttgaacg caagaagccc ggcaaacccg gcgctcgcag gagtttccag ttctcgaaac
    27841 gttaatttgc atccacagca ataatcgagt acaataagcc gccgaatcag gcggcttttt
    27901 ttattcatcg aattttagaa aaaaggattt atacaatgat acgagcagca attattggtg
    27961 ccggcggtta taccggtgta gagtcgatcg aaattctgtt gcggcatccc gaggcgaaag
    28021 ttagctacct gaccgccctt ccggaggaat gcatgcatgt tgcagatatg ttcggccagc
    28081 tccgcggccg tctggatatg atgattgagc cgctggatct tgacaaactg gcttccaaag
    28141 cagatgtcgc tctgtgctgt ctgcctcata aagtttccat gagctttgtg cccaagctgc
    28201 tggccacagg cattaaagta attgatttca gcgctgacta ccgcatcaaa aacgcagcgg
    28261 tctatgaaaa atattatgaa ccccacacgg acaaagagaa tctgtcgcat gctgtttacg
    28321 gcctgccgga gctgtttcga agtgaaatca agggggctcg tctgatcgcc aatccgggct
    28381 gctatccaac aggggcatcg ctggccctgg cgccgctgct gaagcatggc ttgattaaaa
    28441 ccagcggcat tatagtcagt tccgttaccg gtgtttccgg cggcggcaaa aaaccctcgc
    28501 cgaatttcca ttttccgtat atgaacgaaa atatttatcc atacggagtg ggggttcacc
    28561 ggcatatgcc ggagatggaa caggtggcct caacattggc cggaaaaacc gtggaactgc
    28621 tgtttcagcc gcatgttggg ccatttgacc ggggaatttt atccagtacc tacagcgatc
    28681 ctgtcggcac aataacagcc gacgaggtga tgacgctgta tcaggatttc tacaaaggcg
    28741 aaccgtttgt gcaggttcta tccaagcccc ctgccgtcaa acatgttgcc aaaactaatt
    28801 actgccatat atttccaaca gtcgccaaag gcaaaatcgt tgtgttctcc gttatcgata
    28861 acttaatcaa gggtgcttca gggcaggcaa ttcaaaatat gaatattctt tttggactcc
    28921 cggaaacaat ggggcttgaa tgaattcagt gaattcaggt gcagtaaagg ttgctacttg
    28981 tcagtttccc gtatctgcgg atatcgcatc caatgcttcc ttcattcgga cattgatgcg
    29041 gcaggccgcc gagcaagggg ctgagattgt tcatttttcc gaatgcgcct tgagcggtta
    29101 tgctggtgtt gattttgatt catttgaggg atataactgg gatctgctgg cggagaaaac
    29161 ccatgaaatt atcgccttgt ccggtgcact gcacatctgg actgttttag gcagtaccca
    29221 tcgtttgaca gaaccgcata agccccataa cagtctgtac cttatcagtc cagatggaca
    29281 gctggcagac cgctacgaca agcggttctg tacggaaaat gacctcagct attatacgcc
    29341 gggcagtcat ttttcattct ttaatataaa tgacgttaaa tgctcgctgc tgatttgttt
    29401 tgatttacgc ttcccggaac tgtaccgggc attatacaag gaaaacgtcc gctgcatttt
    29461 tcagtcattc tataatgcac ggcagaaagg cccaagcatc cattccgaaa taatgcgcca
    29521 aaccatgcag gcccacgctg caatgaacgg cttttgggtc agcatgtcta attcctgcgg
    29581 ctggttttgc ccatatccgt cctgcttcat tcagccggat ggacgaattg ccgcccagat
    29641 ggatgaacac cgggagggtt tgatgatcaa cattgtcaac ctgcaggaga atttctatga
    29701 tccctctgct ctgtttcggg atatggctat cgccggcaaa ctgaataacg ctttagaagt
    29761 gatagacgat ccccgttcaa ctgatcggca tcatctataa tcacggctta tcttgtatta
    29821 cccctattct gcccgccgta tccgctttga ctatcctcat ctgcccgcga cggagccatc
    29881 agaagatcct caatggaata cacacgtgtc ttattttttt cctgcagccc cacgcgtgta
    29941 ccaagtgtta aaataccccc ttctgcagca aggacaggct ttacttgccc gacagcaact
    30001 gaacgcagga tgctgtccag tacctgcccg agacgcatgc gtccaaaacc ttcgacgccg
    30061 ataggcgtat ctttctcaac caatgcatta gcctgcagat ctccccataa aaccaccagc
    30121 ggcaaacgcg gcactacaga accgcaaaga aaatccaatg cttcagaaaa tggagtatcg
    30181 ctgcggagcg atgaacaatc caccggagta tcaagaagcg tctgcaacga aactctgcgg
    30241 gtacggacag ctggataccg cacatcagca ggcaaaagat ttgcggatat tgctgaagcg
    30301 ggccttggaa gagaggacgg ttgagtgttt tgtgaaaatg cctgctccgg cgtaggtgcc
    30361 aaactgggat tctgcttaat cagtcgcagg gataaatcat tgccgtcagc ccatacatcg
    30421 gcctcgacac gaaagtaatt gcggaaaaca gggttgcgaa gctctatttt tcgagcagtt
    30481 gcaagctgct ctttgaaaaa ctgtgcatcg gaatccgtgg cattctgggg tgaaattcca
    30541 cgtaaaaata cttgaaagcg tacagaagga gcgggttcat accctttcag acggcaaaga
    30601 aaagatacag gtgtaagaac tcggatgaac tcttcaacct gataggtttt ggttgagccg
    30661 gcagctgaaa gcgggcccaa gcagcttatt atgccgacag atgtgagata gaaaaacagt
    30721 tttttccgct gctcacttaa catttatacc caatggttag ccagttttag gagtccttta
    30781 tctggagttc agcaagatgt ctttcaatcg cctcaagctg ctctgttaat tcgcgaagct
    30841 tctgacgcgc ctgctgaaca acttcggggg ccgcccgcgc tacaaagttc tcattcgcca
    30901 atttcgctgc cagcggcttg attcctttct cgacaaattc tttctgtttt tgcagccgct
    30961 gacgttccgc ttcgggatca acaacattat gaacaaaaag ctgtacttct tcaacgatag
    31021 ccgcagcagc attgttcggc tttttaaagt ctggctgcgc ctgaaactgc cctacattgg
    31081 caagctgttg aactaagctg cggttgtcat tcagcacttg gcatacttcc ataggagcgg
    31141 ccgctgaaca atccagcggc tttgacgggg ctatcgtata gcgactgcga atatcgcgaa
    31201 tcgctcgcac tatattctgg acagtctcta tcttcctttc gcttatggag tcaacccatt
    31261 ctgctgtata ttccggccat tgtgcaatca taatggattg ggccggctta agggacatca
    31321 gacccttaag accgcgctga ggtgctatgg agttgagatt ctggaaaata ccctccgtaa
    31381 taaacggtat aaagggatgc agaagccgca gaatctgatc cagcacaaaa gcaagcacat
    31441 tctgggcaac cggtttttgt tccggattct gcatacgcgg cttaatccat tcgagatacc
    31501 aatcacagaa atcattccag aagaacttgt acacttcatt caagggctcg ttaaacttat
    31561 atgcctccag atccctcgat acactggcaa cagttcgggc aagccgggat agaatccagc
    31621 ggtcggtaat gtccatttgc gaagcagtaa aggcatttat atcaactcct tcaaggttca
    31681 tcatcgcaaa gcgggaagca ttccacagct tgttgcagaa atttcgcccc agatcaaatt
    31741 tgggcgaact gtttactttg cgtccatccg gcagctccag ttcggtaacg ggcattcgta
    31801 tatcctgcgt atccgttgtc atgctggcta aggtaaaccg cattgcatct gccccgtgac
    31861 tgtcaatcgc caccaaagga tcaataccgt tgcccagact ctttgacatt ttgcgaccaa
    31921 agccatcctg tatcattgca tgaatataaa cgtcgctgaa tggtatatcc cccacgcagt
    31981 attgccccat cattaccatc cgcgaaaccc acagcgtgat aatttcccgt gccgtacaga
    32041 gcacatcggt cggataaaac gttttaaggg tagcattttc atccggccag cccatcgtgc
    32101 tgaacggcca taatgcagaa gaaaaccatg tatccagcac atcctcatcg cgcagccagc
    32161 cttcggcatc gagggcttgt tcgatttctt cacaatctgc tttcacacag gcgtacgtca
    32221 acgactgctg ttcgttttga acaatggctg taaatacagc gttggggaag ggatttttat
    32281 gattgccttc ttttgaccag accggtatcc gatgtcccca ccagagctgt cggctaatag
    32341 gccagtcgcg aagattttcc agccatgttt gataggtctt ggcataccgc tggggataaa
    32401 atgtcagccg gccttccagc aacggtacaa gtgccaatcc agccagcgag ttaaccggaa
    32461 catcagttcc tttaatgaag gttacgccgt cccgcgtttc cgtttcgcat ttgacttctg
    32521 gcagttgaca gctgatattg ggaatcggct ttttaacagc aatataccac tggtctgaca
    32581 aatacggttc aatcggcaca tggctgcggt agctgtgacc gacttcatgg gaatattccc
    32641 gcacatcttc cagcaggttt tcctgcctga accattcgac aatcgcttca cgggcttcaa
    32701 aacggtccag tccaatcaca ttttcgacat ccggattttt aatgtcttcc cagtccgtcc
    32761 agccgtattt ttcgctgatg gaaccgtccg gtgccatcac attgattaca gccaaaccat
    32821 gccgctgacc aatcgcccag tcgtctggat cgtgcgccgg tgtaaccttc aaaaaaccag
    32881 ttgaaaactt cgccttttcg tctggactgt ctggattggg cagaacgaca tgttcatcag
    32941 cgataatcgg aatcagccgg ccgactatcg gcaagcgcac gattttgccc acaagatatg
    33001 tagcccgcgg atcagccgga ttcattgcga ccgccgtatc ccccagcatt gtttccgggc
    33061 gggttgtcgc cacggtaaca tattcaactt ttcggccgtc gataatagca ggcgtctgga
    33121 gaggatattt cagataccag aaaaatcctt gaatagtctg atgctcaacc tcatcatccg
    33181 ccaaaacagt ttgcgtggcg ggatcccagt tgacaagccg cttgccccgg tagattaatc
    33241 catccttaaa gagattgaaa aaaaccgttc gtacagcttt ggcacagact tcatccatcg
    33301 taaaacgcgt ccgcgtccaa tcacaggaac agcccatact cttgagctgc ccgagaatgc
    33361 gggcttcata ctcatccttc caagattgaa catgggagac aaattcatcc cgtctgaaat
    33421 ccgtgcgccg cttgccctgt tcagccagaa ggcgtttttc cacaacggtc tgggtggcaa
    33481 tacccgcatg atctgtaccg ggcatccaaa gcgtgttata cccctgcata cgcctccacc
    33541 ggaccagtat atcctgaagg gtattgttca gcgcatgccc taaatgcagt gccgccgtaa
    33601 cattcggcgg tggaatcaca attgaatagc tgcccttcgg cgaagccgct tgttcatcgg
    33661 catgaaaata ctgtcccttt tcccatagtt cagcggtctg acgctccaca gaagacggat
    33721 cataaatctt gggtatctca cgcatcattt ctcaattcct gtcttgggct ctttttcaaa
    33781 taaaaagtcc cggcatattt ctggccggga cttgtattgt agccaatatc ttttggttgt
    33841 aaagggttag tccaatttga gcgtcatcag aaattcagca ttggaacggg tcttggccag
    33901 acggttcttg agcagttcaa tggcttcaac catattcatt tcgctgagta cttttctcaa
    33961 acgatataca agctccattt cccgcggatc aagcaggagc tcctcgcggc gggtgccgct
    34021 cttgctgata tcgatagccg gaaatgtccg ccgctcgacc agtttgcggt ccagatgcag
    34081 ctccatattg cctgttgcct tgaattcttc aaaaataacc tcatccatct ttgagccggt
    34141 atcaattaag gccgttgcaa tgatggtcag tgaaccgccg ttttcgatat ttcgggccgc
    34201 accgaaaaag cgtttgggca tctgcatcgc attggcatcc acgccgccgg tcaaaatctt
    34261 gccggaatgc ggcatttcgg tattatatgc ccgcgccaaa cgggtaatcg aatccagcag
    34321 aataacaaca tcttctccat attccaccat acgtttggcc ttttcgataa ccatctccgc
    34381 aacctgacag tggcggctgg tcggctcatc aaatgtcgag ctgatcactt ctacatcatc
    34441 ggccgtcttt cgctgcatat cggttacttc ttccggacgc tcatcgatca gcagcacaat
    34501 gagtttgact tcgggatggt tatgactgat ggcattggca atcttttgaa gcagaaccgt
    34561 tttgcctgta cgaggcggag caacaatcag gccgcgctgg cctttgccga ttggagtaat
    34621 caagtcgata atacgcatgc tcagctcttc cggcgttgtt tcgagaatca gccgttcttc
    34681 ggcgtgcagc ggggttaagt ctcggaaaac aactttttcg gccaacttat ccggctcttg
    34741 atagttaatt gcctcgacgc gcagcagggc aaagtatttt tcgctttctt tggggggacg
    34801 aatctggcct tggacaatat tgccgctgcg aaggccaaac cggcggatct gggaaggaga
    34861 aacatacaca tcatccggcg aagccagata gttgtatttc ggactgcgca gaaaaccaaa
    34921 gccgtccggc agaatttcca atacaccctc tccatacatt aagccgtcct gacgaatccg
    34981 ttctttgaga atgcggaaaa tcagctcctg tttggtcatc cccatgtaat cggtaatgcc
    35041 ttcctttttt gccaaagcat gcaaatctgt aacttcaaga ttctgcagat cggcaatatg
    35101 aaggttgccg cgtttaatgc gttcatactt agcatgggtt gattcttctt ctgtaagggt
    35161 ttttggttct tcggctgcag gagcttctga cgccgctaat tgtttttctt cggcggaaat
    35221 gtcttgggat atttcctcaa ccgtttgatc cggctgaatc tcggtctgct ggctggactg
    35281 ttcaggggct tctggctggg tcgctgtatc tttcggattg cgtttctttc ttgtttttgc
    35341 cgttgtcttg gccattgttt ctcctaattt atagaaatag tttcattatt ctatcctgta
    35401 aagggtcaaa atcacaggaa tgcttattaa tcttctgcct taaagcagct ctacttactt
    35461 tttatggcaa ccaaccacag cttctcgaaa ctgtgtgtta tgcgatggga tttcaacgct
    35521 gtatctaact ccatctatgt aattactatg aatctacatt tcggttattt cggcatataa
    35581 aattaacaat tgcccgcccc tgctctgcca aggtatttta ccgatataaa ctacttattt
    35641 cctgtaattg ttgagaagat ttttacgact tgctctgcca gctctgactc gtcagaattg
    35701 tttataatcc tataatgtgc catttgccgc tttttgtcca gagaaatctg gaatttttct
    35761 ctttttttta tctgtaagag gtcaattttg ccgtttttgc aaactctctg caattttttt
    35821 tcttcatcac attcaacaaa aattaaaaag tcgcactttt tatcccatcc cacttccagt
    35881 aaaagcggca tatcaatcac aattccccgg acattggggt cagtactaaa ggaatctaaa
    35941 agtatttggc attgctgcag aacccgaggg tgaattatcc gacacaaaaa acccagttcg
    36001 tcgggattcg agaatacctg ttctgcaatt tttttacgtt caatcaagcc tgaagaattc
    36061 aatatttccc tgccatacct atccgttata tcccgaataa tttctggatc attcagaact
    36121 tgatgggcga ttgaatcggc ttcaatcacc ctgcacccaa gacgttcaaa atgtttggca
    36181 acggtacttt tcccagaagc aatgccgccc agaataccaa taattggtgg tgtcatcgag
    36241 gcaaagttaa tcaatgtttg agagtgtgtc aagaaaagaa atggggaacg ctgttagcgt
    36301 tccccagaag accagcccta ttattcatca aagatgtcat accacaaccg cctgcatagt
    36361 gtgctttttt ctaagcgctt tattcagact cttaagcaca tagttttgta caacgctctc
    36421 ccagcccatg tgacgggcca attcgtatcc tgactgcagc aggtggttca attcttcgtc
    36481 atttttgggc agtctggcac agatttccag agcaactttc tcgctgattt tgtgttctat
    36541 tcgatctcga taaagccgat caatacgcag catttcttca acatccatgc cggcgtcgcc
    36601 atttaattcg gtataatcgg caaaaataac attcgggacc atctcgctgc cggcaacggc
    36661 cttgacaaag cctgcgcagc cgcagacatt ggtcacaaca caaatgcctc cgaaagtaag
    36721 cggctcaagc tgcgcaatac caaacggctc atatatgctt tggccgaatt cgacatcgct
    36781 gccctttcgt atatccataa actgcatatc cgcaggcatt cggtagccgc aggaggcctg
    36841 gtcaaatcca aactgattaa taaagataac cttgatattg cggcttttgg cattaaattc
    36901 ctgaacaccg acataaaaag ctgcttcacc cccggaaaga tccggcattc cttcgcgatg
    36961 cgccaccggc catttatacg cagcttccat ttggtaaatg tcctgctccc gccggcgatg
    37021 cgtttctgta ctcaagacaa gcagaaccgc tgtccggttg agtgcattca attctcgatc
    37081 catatgttcc aaaacacgca gatcgcgcca tagtcccttg cttaaaacaa gccgggttac
    37141 atgagtaaaa acaaaatccg gcttccaccc cagcagattc tggcaatact gccgcagctt
    37201 atcccgtgat tcatgttttt cggcaactgt aatttgataa gcgggaatgc cgttgtaggt
    37261 caaatcaata tcggctgtgg cgaattcggg cgccaagaat cgtaattcat cgactacaca
    37321 attaccgacg gccagaatat tatcacaata gcgggcggcg tttaccaatg catatttgaa
    37381 gaaactgctc tggtccccaa aaacttcatt gacatagagg tctcgctgat gagctttttt
    37441 gattacatta taaaacatcg tatcgtgtcc cggatgctct tcaacaattt tccgcatcgg
    37501 agccacttcg tgcgcgtaaa aaaccgtctt gaaatcaaag ggatccaaaa cagcagccaa
    37561 tgctgtaggc atgcccataa attcgtgggc gactataatc gtcggcttat ccggctcggc
    37621 tgctccgatg gccttcagcg cttcgatagc aggaagcgcc agccgcaccc attgctcata
    37681 atcccaataa tgctcatagc ggtcgcttcg gataccgaac tccctgaaca ttgccccctt
    37741 taactgatca accggcgatt tctccatccg ggttacatca atcagcagaa tctccggctg
    37801 gccggcaatg ccgttcatcg gatcggtaaa ccgtcttcgg ccatagacaa tttcgacatt
    37861 gaatgcatct tcaatccgcc gaaaactgga aacataactg cttcgaatta atccatccag
    37921 agaagaatac aaaacttctc cgcccggccc cagacggcta aaaacatttc cttctgttga
    37981 aaataatggg cttattaaaa ttgttctgcc aaccgcctca ttataggcgg ggctggtaaa
    38041 cattccttgc agaacagctc caataccccc gatttttcct gtggcctcgt gagtaacatg
    38101 cacaaccgtt ggtttttgaa ccatttttta aatccccatt ataaccttgt tacattgttt
    38161 gcaaatactc tgttgctgac aaggtttcct tgtctattca taataaaata tagagaataa
    38221 aagtccactt gaccaaatat atatcggtat ttttacggtt agaaaattaa gtggttcgtt
    38281 cttatctgaa atactatttt ttaccggacc aaatatatta attccaatgt attttaatca
    38341 tgtatttcaa ataggtgtcc taaccatttc tgatgtaaaa ttgtttagtt ccagaaatga
    38401 taaattcagt gatgatggcg tcagtcagaa ttacccagca gccgcttttt tcaattctat
    38461 cctgtttatt ccgagacttg gataaaggca tgtcccagat gctcaacaat gtcggaagca
    38521 atcaggctaa cttgtccgcg gattttcgca gcagcatcac cagccaaacc gtgtatatac
    38581 actcccagta cagccgcatc aaacaaagac aatccctgcc ctgccaatgc cgcaattaca
    38641 ccggtcagta catcgccgct gcctgctgtt gccatgccag gatttccggt gtcgttaaca
    38701 tacaggcgat ctccatccgc aaccgttgta ccagctccct tgagaacaac aatgctcttt
    38761 gatttccgag caaattcaga cgcacaggtt tggcgattct ccggcatcgg ttcacgaaaa
    38821 acgcttttcc acagccgctg catctcgccg ggatgaggag tcagaataac atctgccttt
    38881 ttcttttcaa gccatccccc ggaacccccg cactggctga gcacattgag tccatccgca
    38941 tcaatcacta atttcaaacc ctctgcttta atcaaacaaa gcagcgtttc cttaacacct
    39001 gcgcccattc ctacacccgg tccaaaggca atgacatcat tttcagcaag catttggttg
    39061 ataaccgccg catcttgagc ggacatccgg ccgtttgaat cttgcttcaa tgcaatcgtg
    39121 gtatagcaag gattgacagc cgcaacaatc ggctgcacag aagcgggcac tgcgacgcgc
    39181 accagtcctg ctccgctccg caaagcagcg attcctgcca gcgccggggc tccgctgtat
    39241 cctatactgc cccccacaat cagaaccttg ccgaatgttc ccttatgacc atcagccggc
    39301 cgcggggaaa gctttggaat aacagtaatt gtctccatct tcgcaagtca taaataatgt
    39361 tagaacagct ctttctgctc gattttatac tgaatcagct gagcccgctt tcgagcagcc
    39421 tgcgtcaaaa acttctctat tttcttctta tcctcactgc gaacagcatc ctgaatcagc
    39481 cgtaattgtg taatcagtct ttcaattcct gcaacacagt tggccggatt ggtcatcaga
    39541 atatccgtcc atatatctgc tggcccggaa gcaacacggg tggtatcaat aaagccgcgg
    39601 ccggcaaact tcatatcctg cagtgtattc gcattcacaa gagcggctgc tgtaaggtgc
    39661 ggtagatggc tgactctccc gagaatacga tcgtggtttt ccggtgacat cactataacc
    39721 gtacagccca gtcgtgacca aaaccaccga agaagctcga tagacgttgt acttgttcca
    39781 ttcgtttttg ttaatatgca tcgggcattt gtcagtaaat catcgcgggc aaattcaacc
    39841 ccacgtttct cagagccggc tatgggatga gagccgacat aactgacctt tttgggcaaa
    39901 tattttttag cccaatggtg cggcagcagt ttggtcgatc ctacatcggt tactatacac
    39961 ccatctttca aatatctcga tatttctaaa aagtaattct tgaatgtctg tattggtgtt
    40021 gccaaaatta caatatccgc attttttact gactcctgca aggtttcggc aatttcatgt
    40081 gcaactccgg cctcacgagc cttctgacgc gtcttggcac gatggctgta gccaataacc
    40141 tgtgctgacg gcaactcccg ctttacagca agggtaacac ttgcccctaa gagccccatc
    40201 ccgatcacac taatctgttt taagttaatc atatttaaga acttacaata aacagagaac
    40261 aattggcact gcttaaataa gtaccttgct tgtgttgata tgtcaatttt tttcttcctt
    40321 ttgacctatt aaatatggta ttatttaaga accattggtt gccggtaagc ggcaggaaaa
    40381 acattctggt tcgcccttga ggcgaacagt ttttttaaat ttaacaaaaa gctgaatcga
    40441 atgaaaaaag aagatactat agatacaata aacattgtaa aacaatactt gccgttcgaa
    40501 gaagaaattg ctgctatcga tcggcagatt gaaatgctca aacagcaaaa cggacaaaat
    40561 tcgctgcagg gtaaaaacgg ttatcaaatt cgtgcgctcc aggtacagca aactgaagtt
    40621 ctcaaaaaaa tctactcttc tttaactcca tggcaaacag tacaagtttc gcggcacccg
    40681 cagcggccga ttttaagtga ttacctgaat cggatgttta aggatgttcg ggagcttcat
    40741 ggagaccggt gctttgggga tgatcgggcg attattactg ctttggggca aatcgggcat
    40801 caaaaagtca tgattgtcgg ccagaataaa ggccgagata tcaaggaaaa agtggcctgt
    40861 aatttcggct gtccgaatcc ggaaggttat cggaaagcac tggctaaaat gaagctggca
    40921 gaaaaatatg gacttccgat tgttacattg attgatacgc caggcgccta ccccggcgtg
    40981 ggcgctgaag aacgcggaca ggcacaggcc atcgcagtaa atttaatgga gatggcgcgt
    41041 ctgcgcacac ctatagtatg cgttatcatt ggagaaggcg ggtcgggagg agcattggga
    41101 attggagttg gtgataaaat cgctattctg gaatacgctt actattctgt catttctccc
    41161 gaagggtgtg cggctattct ctggcacgac ggaacaaaag cccctcaggc agccgaagct
    41221 ctaaaattga ccgcaaagga tctgcttaaa cttggactgg tcgactctat cattcctgaa
    41281 ccgctgggcg gcgcacaccg aaacattcat gacaccattt ataatgttga gcaatttatg
    41341 attcgcacgc ttgcgcaatt aaaacgcttc agcattgacg cgcttcttga gaatcgttac
    41401 aaaaagctgc ggcagatagg tgccgattgc gttaaaatct cttctcttac ttctgccacg
    41461 gacaccaaac gaaccggttc agaccttgcc ttgctcaagg aaaaactgga caaactttca
    41521 tcggaacaga tatcagcttc tttcggggta taaaaaaccg attctggtat tggattggtg
    41581 ttttgaattt atgcctcagt gttcagctgc cgaggatcca ttccttcttc aatggcctgt
    41641 atctttctaa cacgccgcag atgacggcct gcaacaaact ctgttgtcag ccatgtttcc
    41701 acaattttcc gcatcatcgt agggccaatt aaatctccag acagacacag cacattggca
    41761 tcattatgct cacgcgacag tttggctgcc agttcgtcgt agcacaaggc cgcccgaatc
    41821 ccttttactt tgttagcggc aatgctcatc ccaatccctg ttccgcaaac aagaattgcc
    41881 cgatcggcct ttttttgggc cactgcggta gatgccgcat atgccatatc cgggtaatct
    41941 accggctggt cagcgttgat ggaataatca atacattcaa atcccagctg cgatatgatt
    42001 gcttttatct gctctatggc attaatgcct cgatgatcgt tggctagcgc tactttcata
    42061 aaagttcatc cgttctctct gctatggccg tttgtatctg cctgaaacat gcttcataat
    42121 cttccctgtc caacccaacc ggatcctcta tcggcatccc ccttttatcc aaaagaaaac
    42181 acctgctgtc ggccgatggg cacagccgca ctacctcatc cctatgatgg ggactcatta
    42241 cataaattac atcgctctgt tcggccaaat ttcttgttaa aagccggcta tgatggcctg
    42301 tcaagtcaat tttatagctg cggcagactt caactgcata gcggcttgcc ggggcaccat
    42361 caaaggcgac aacccctgct gagtttatta tatatcctaa acggctaagt tcgtcaactg
    42421 aacagcccaa aatatcagca aaatatttcc tgcaaatacc ctctgccatt ggactgcggc
    42481 aggtattgcc agtacataca aacaagatac gcaaagtcgc catatctcgt attacgctgt
    42541 ctgggacggc acccgaccga aggatgtgta tgccgctttt accaacctta accacagtac
    42601 tgctcttgtt ataacatatt ctttcgtcag gggtatcaac aatgcattca attttaccat
    42661 taaaatatgt caaaacctgc tcagcattga ttgccggatt ctgaccagac aggttggcgc
    42721 tgggagcaac cacaggaaat tgagacgacg aaaggatagc acaggcaatt gaatgcgccg
    42781 ggtaacgaat cccgacagtt ccatctgagt acaacaaatc aattacctct tgaggcagag
    42841 tttgggtgat atgctttaat cccttttcgt tacattcgaa tacaattgtt actggaccgg
    42901 gcagaatacg gctcacaagc ttgcgggctt gggcattcat tacagggacg tattttgaca
    42961 gctgttcata attgcccaca tgcagggtat aacgcttgtc cgcaggacgc ccttttaact
    43021 ggttaagacg ctggattgta cttaattcca ccctgcagcc aaccccataa acagtttcag
    43081 tcggaaatgc aaccagtgcc ccttggtcaa gcaattgtgc cgcttcttga acagtttcat
    43141 ctttcggatc gtaaattttt aggatttttg tctccacaat actctctttg caaaatccga
    43201 cagcctgaaa tcattttaat ttccggtttt caaaacaccg ggaattgtag agagcgaagt
    43261 ctcaatctga cctgttgcaa ggttgtatcg aacagccggc acgcgtgctc catccagccg
    43321 gcagggattt gcttccgttc cgtttatggt cagccatccc cctccggcct gagcatcata
    43381 gtccaatgtc tgcccaatca tctcgtgaag tgttttggtc tgatcggcat tattttcccg
    43441 atatacaatt ccctgattgg taaaaacttt tgccaacata gtacggccgg cggaatcggc
    43501 cgcaaaatcc agattaaact ggatcgaatt gacatagata aatcgttccg gcttcttctc
    43561 aagcagcggt atataaacaa gctgcatagt ttcctgattg gcgtcagcga cgattttctg
    43621 gtcgtttagc agccaccgga ttttgtcaaa tccaaccaac tgagctacac acggccgccg
    43681 gaagttgatt cccgtctgtt ccggcatatc agccggtgtt tcatcgctgg ttatcagttc
    43741 gattttgccg gggccttggg ccgccaaaag attttcagga cgatcccatg tgatgtcggt
    43801 gcaagtcagc ttaacatgag acaatttgac atcttcgtgc atgcgtagag attcggcaaa
    43861 tacatttcct tcggtaatat tgatacgctg tatatccata gagccggttt tgtttttttg
    43921 cggaaaaacg gacagttttt ctccatggaa ggtatctgtc tggatataat tcaatgcctc
    43981 caagcggcgt acggctacaa cattttggtt aaacactacc tgccggatag agccggatgt
    44041 gtcggctgta aattctgcat ttttctcagc cgtgaccgtt atagggatcc atggctggag
    44101 ggcttgattg tcattttttg ccgcttgata aaaggtaaac tgaagcgggc cgtgagccaa
    44161 tcccgaaccg gtttgcagat cgtaatcaat ccgacgtgct tcaaatcggg ccggcggttc
    44221 ctctggcgac gctgttttgg tttgtaaaac tgtattgccg gaggcatgaa cagctgcggg
    44281 ctgaacagct cctgatgtat cggctgaaaa ttcaattgtg gcatcggatg aggtgagctg
    44341 ccctgtctgc gatgctacac aaacatcgcc ttggagattg gcccgctgaa ctttatcctt
    44401 ttcaaaataa aaaacggcgg tctcagcagt tgcacgggac tggctgctgt cttgaaggct
    44461 ttcgaaacgg acctgaccgt tcatataggc agctgtcaga gaatttccgg gaccgaattt
    44521 gagggttgcc gttttcgcgg cagcccgctg cggtctgccg ggctccagag actcaaattg
    44581 aacgcctccc tccaggtgcg ccgactcgat gtcacttttt tcgccaaact caaaaacagc
    44641 tgaatcagct gcggtcttat accagccgtt ctgcctgaaa attgcattca tgccgccggt
    44701 taagttgaca gtctgcacag ttacagaggt tcgagctgtt tccggctcgg caaaccgcag
    44761 ttccattttg ccgccaaagg ccacctcgct gggttcttgg gctgaatctt tgctggaaag
    44821 aaatgctttc ccgggacctc caatgctggc ttgctgtgtc tttcgattcc acaggacatt
    44881 gccgcgcgtt tgaatccgcc cctgcaaaga cggatctccc agtacaatct caggctggct
    44941 gatgttcgta aacaaacgca gaatatcctc ggcgggctta tatttaagaa tgccgcaaga
    45001 aaccagcatt tcgttgacca cgccggtttc tgacgaaggc cgctcaatcc gaagcggcga
    45061 gccgttcatt tctatcgata gttcgggagc agcttcagac atggatgaat tgtcagcacc
    45121 gttcatcggc tttaggataa tgccgccatc gcacaggacc actatatccc gatttttact
    45181 gtctgccgag cttggggcag tcaaggaaat gccgggagac ttctgtccat ccgtcaggga
    45241 gtctgcagat tttacaggcc cttctttttg cccagctgcc tttttgtctg tactgtcgat
    45301 tctcgataac aggatatttt gcactgcaac gcggtcggca ccggcgacaa ccagctggct
    45361 gccgtagtga ataacaacat tgttatctaa aatgcattga tagtagtctg gctgctggct
    45421 ggatgtttcc attgattttg atgtcgagga cttcggagaa cgtacggcag agctaaaagt
    45481 atcggatgca gcatttttag gtgccgctgc agaagaggag ccctcggatg ccgaagcgga
    45541 ggatgccgcc atattaatca gccgcagctg ctccaaatca aggatatgca gataatcgat
    45601 ccgccctttg gcgacatcca aaatcagaac cagaccgaat cctttcagat caatctggtc
    45661 tgaacagact cgcacaggac catccgtggc aaattcagac cgttcactgc tgaatgtcag
    45721 gtcatccata taaacaaagg tttggctgat tgaacttcct gcgcggggag ttacacgaat
    45781 gacaacattg ccgtttagct gtgcatcccg gacatccttg ggggctgcat tggggccggc
    45841 tgtctcaacc tgaaatactc cggagtcgga ttccagccgg cattcataat ccatttcata
    45901 gaaaatcaaa tagggttttt cgaccagcca gcgtgacgat tccgcctgcg gattcagcag
    45961 cttctcaaaa cccagaacgc ggcgcacttg tttggtttga ggatccagca gcacatagcg
    46021 ggactgctgg acgccgtgaa taaccgtctg acttgtttgg ccggcatcag aagcttgggg
    46081 cagggtcaga acatttgcat cggccgcctt agggggcagc ggcacagaag tttccatcgt
    46141 ccgcacatac agccagaagg ccagtgccac tgcacccaat gaaatcagac caatacttat
    46201 tttccgcata cttcgctcac cacaatagga acaaaataat tccgcatcct gttaaatcct
    46261 gtaccgctcc ataagtgaat cccatttccc ggctgctttc agcagatatt caacaacctc
    46321 tccgaccgca ccatggcctc cttgttttac agtgatgtaa tcggccaact gctttaattc
    46381 ttcagccgca ttggccactg caacaccgaa tcccacgcga cgaactacag gtatgtccat
    46441 aatatcatct ccaatatagg ctgtctggct aggcgcaagg gctgtttgtt gcaggagctg
    46501 ctcaaatgct ggaagctttt ccaaacatcc ctgcattaca tacgcaatat ccagctgacg
    46561 agcccgatgg attgtagagg gcgtgtctct tccgctgata attgccgtaa gtccgccggc
    46621 acgatgccac atcttaatac gatgtccatc catcacattg aagcgtttgc tttcaatgcc
    46681 ctggtcattc aggaatatgc ctccatcggt cagaacaccg tccacatcca gaatcagcag
    46741 ttttatttgc gccaggttgt ctgctgccat cgaactatcc tacaatttta atcgttacaa
    46801 tatcctgcac atcaatcagg cctaccggtt tattttcaga attgacaacc ggcaactcat
    46861 caatacgata cttatgaaat atcgccatcg cttcagcagc cagtgtatcc tgacggacgc
    46921 atttgcagtt gggggtcata atctgccgac agaccatcgc aaatgtcttc tccttcatct
    46981 tcgccatcgc ccggcgcaaa tccccatccg taatgatgcc cgcaagccgt ccctgccggt
    47041 ccacaaccat cactgctccg cgccgcttca gcggctcatt cttaatcagc attgtttcta
    47101 ttgtatcatc catttccgcc agcggcagcg gttcatcggg tttaaaccac attgcctgcc
    47161 ctacggtcag cagcttgcgt ccgagtgcac cggcaggatg aaaacgggca taatcttcaa
    47221 tgctgaattg acgcgatttc atcgtcgtca aagccagagc atctccaaga gccagcatgc
    47281 aggtagttga aacactggga gccgcaccaa atgggcacgc ttccgtcagc attcccatac
    47341 acaaagccac atcgctgtgc acagccagtc gggattgggg atcgccaaca atagaaacca
    47401 gtttaatctg ccgctgcttg agcggttcta tcagccggac aatctcttcg ctttctccgc
    47461 tgtggctgag ggccaataca atatcattgc tttgaatccg tccgaggtcg ccatgaaccg
    47521 cttccgccgg atgcagaaaa tggctggccg ttcctgtcga agccagtgtg gcgctgattt
    47581 tgcttcccac aatgccggct ttgcccatac ccgagacgat aaccgaaccc tggcattcaa
    47641 gaaacatctg tacaacctgc ctgaaagcag accagtcgat cgccttgagc aggcccagca
    47701 cggcctgccc ttcggactca atcacattct tagcaaaatc gatatcaatt gctgtttctg
    47761 ccatgttatc tccgcaggcg tctgcctgag ggcagcatgt cattagaatt tgacaacact
    47821 gccgcgttca tcaaaacact caattcgggc ctgccccaat gtcccggaag tatcgggatg
    47881 gattcgaatc tctttgccgt tttccgcctc aatctgcgcc agtacagccc gtcgctggtt
    47941 gagaagaaaa tcggccgctt ccggagatac ggttgcctcg atgcgatgaa tttctttttt
    48001 cattgcggcc attcgaatta tccgcagaac ttcgatagac tgcgactcga tactcttgat
    48061 gacgccggtt ccgctgcaat gcggacattt cagatacgtg ctcgactgca gggatggacg
    48121 catgcgctgc cgtgtcagtt ctatcagccc gaaagcactc atcttaagca ctttggaacg
    48181 cgcccggtcc gatttgaggg cgttgcggaa ttccttctca acttcgtgcc tgttgcggct
    48241 gtcccgcata tcgataaaat cgcagataat caatcctccc aggtcgcgca gcttcagctg
    48301 ccgcactatt tcgcgggttg cctccagatt gattttcagg gccgtctgtt cagcattggt
    48361 ttgtttgcga tagcggccgc tgttcacatc gatcgccacc agcgcttcgg tttgttctat
    48421 caccaaagac ccgccgcttt tcagttcgac ccgcgtggac tgaatttttt cgatttcctt
    48481 ctctatattg tatttgtgaa aaagaggggt gtcgccttcg taaaagctga ctcgatgctt
    48541 cagccgcggt tgcacaagag acaaaaaatc ccgaattttg cgcgtttcag cttcattatc
    48601 acagagaatt gtatcaatag aagaatcaaa aatatcccgc accgtacgaa gtgccagatc
    48661 ggattcctga taaatctctg cgggggcttt ctcggtatcg atccgtttgc tgattacccc
    48721 ccatagccgc ttcagatagg caatgtcgtt ctgcagctcc cgcttgctgg ccaactgcgc
    48781 cgccgtgcgg ataatcagcc ctgcgttctt tggaagaggt atgccttcca tcatgtcacg
    48841 cagtttttta cgctcctgtt catcttcgat tttttgggac acacccacac tggtcatcca
    48901 cggcattaaa acaaggtact ttcccggcag cgaaatatac gagctcagcg tcggcccttt
    48961 tgtgttaatg ccttctttgg tcacctgtac aattatttcc tgcccttttt tgagacatct
    49021 ctgaataggc ggtctctgag ccagagcttt acgccggccg atgttttctt cactctcttt
    49081 ctgcccaaaa taacgcggat gcaggtcact gatatggaga aaaccgtttt tcgatgtacc
    49141 aaagtcaata aacgccgcct gaatggccgg ttcgatattc accacccgac ctttataaat
    49201 gttgccgaca tggctaacca tattggaacg ttctgtgtat aattcctcca aagaaccatt
    49261 ttcaaccacc gccacgcggc attcttctcc ttctgtaata ttaatcagca tctcacgcga
    49321 cattcattat tcctctttcg ctatcaattt tgttcccatt gcacttccat acgcaagacc
    49381 ggctccctca acatcggctg gtcaagatgc agccattgca tcatctcatc gactcgaact
    49441 gtaccggctg ggctgacgcg gcagaccagt tcaatccggt cagcaaaaat atccatcttc
    49501 tccacaaacg gcgaaatatc cagcttaaca aaatgccctg ttttggcatt cagccgctgt
    49561 acttcaatag gccttttatc ctggatgctt tgtcggcact gctggcaatg ctgcatcagg
    49621 gctgtctcgg ccggcggttc tacgaaaaat gcataacgaa cacttctcgg ctggaaagtt
    49681 attttgccct ctctgcattg tatctgacga attgtgcagc cgacgggcaa ttgccgctga
    49741 atgcccccgg acaggtcttg ggggattata cctttggaaa atctcactgc ggcacacaga
    49801 cgctctgccg ctgactgtac cccgacactc cgcggaagcg gaatcgacag atgaggccgc
    49861 ggattaaatc cgctggaaaa aatcagcggc acttcggcgc ggatcatcgc ccgttcaaac
    49921 agggtcagcg tttcctgatg ggacagatac gctgcatttc cggttataga aaaatccgca
    49981 atcagtgtgt aaacctgttc gctcattcct ctctttgaat gcctcttggt tttcctgcgg
    50041 gcattgtccg ctgcatccgg ccgtgcctca catcatcttt ctgcagggca aaagcaaatc
    50101 ctgcctgtta aaaactctcc ggcagaatcc gggttgcagc atcccgcaag taattcttaa
    50161 tctgccgcgg agaatccatc gttgcaacct tgcgcgacag ctcattgcac tcctccattg
    50221 ttactgaacg aataatcttt ttgacttcgg gaatcatcgg cggcgccaat gacagcgtgc
    50281 gcacacccat ccctatcagc agcataataa attctggatc cgatgcgact tccccgcata
    50341 tgctcaatcc gattcctgcc ttataggcat cctgaatcac attacggatg agcttcaaaa
    50401 cagcaggttc cgccgctgaa tacagtgttg aaacatgttc attggcacga tctacggcta
    50461 atgtatactg gattaaatca ttggtgccga tgctgaaaaa ggagcattcg ttagccagca
    50521 aagcagcagt cagcgccgag ctgggggttt ctatcataat gccaaccggc atttgggcat
    50581 tgtacggcac agcatattca tccaaatctt ccattacatc ccgcagaatc atcttggcct
    50641 gccgaagttc ctgcaggttg gtaatcagcg gaaacataac ccgcacatcg cccagcacag
    50701 aggcccgcag gatagcccgc agctgcgttt taaacagcgg cagtgcctgc aggcagtagc
    50761 ggatggaacg caggcccagc acgggatttg tttcattcgg atgccatccg gaatgcggaa
    50821 gtttatcggc tccgaggtcc attgttcgaa tgacaacggt tctgccttca actgcccgaa
    50881 tagcatccgc ataagcactg tagtggtctt cttccgtcgg ctcctgcccg ccgtataaat
    50941 acagaaattc tgttcggtac agtcctacac cctggccgcc tttgctgata accacagcgg
    51001 cttcgtcggg aaactcaatg tttccaagga gctgaatctc aacgccgtcc ttcgtctgtg
    51061 caggcaggtc tttcagctcg ttcagcttac tgacaaactg tgttatctgc tgggcatagc
    51121 gatcatattg cctgcgggtt tcttcatcgg gatcgattac aacaacaccg cgattgccgt
    51181 caacaataac aacacagtcc ggcggtatct cggcggacaa gtcccccaat gcaaccacag
    51241 cgggtatccc cagcgaccgc gcaacaatag ccgtgtgact ggtgcgtcca cccttctctg
    51301 tggcaaagcc cttaataaaa gttttgttga agccggccgt ctgggtaggg gttaaatctc
    51361 gggcaagaat gacaacctct tcccgcaagt gatccagctc ctcctgacgc ttgccgagca
    51421 gatggaccaa aagacgtttt tcaatatcat aaatatcagc cgcccgctcg ctgatataag
    51481 gatcgtcaat tccggcaaaa tgggatgcga tttctctgag aatggcggaa acagcatatt
    51541 cggctgtcac caattcgtga ttaatcatat cggttatgcg ccgtctgaag ctccgatcac
    51601 gcaaaaaccg gaggtgtaca gcaaaaatat cacggatttt gtcttcctca atattcttgg
    51661 ctgacttgac ctgttccagt tctgctacag ccgtagcaaa tgccttgcgc agacgctgta
    51721 tctcatgcgt ccgctgtgaa ggcagaatcg agcggcgagg aatgcggtaa tcctttgaat
    51781 cgatcagcat tggctttgct atggcaatgc ccggtgaaac cgcaattcct tttctaattt
    51841 ccatctgtgc aactaccctt ccttttcgcc tgcctctaca ggtgaatcgc cggcgaattt
    51901 ctgttccacc agccgcttga gagcttcaat ggcctgtcgg gcatcagccc cgaccgcctg
    51961 aatccgcagt cggcttccgc aggtcgccgc cagcatcgtc atttgcataa tgctcttggc
    52021 gtccacagtc aggtcctgat gactgacagt aatatctgaa tcaaacgaat tagccaaatc
    52081 cacaaactgc atggcaggac gcatatgcag tccttcagga tttagaatct ctacctcaat
    52141 ttcgtggatc ggcttggaca cgttattatt cctgtatctt ttttacagca ctaaaattta
    52201 aaagttttca ctaaccggca tcttcatcgg catcccgaat cgcctctata atttcttgac
    52261 gtgtctgggc ttgccgcagg aatcggcgga aatcgtcttt ttggagattt ttgaaaatcg
    52321 tttccatcgc ctgtaaatgc ttatccggct tgttggtcgg actcaaaagc agaaaaacag
    52381 aatagaccgg ctgcttgtct aaagaagaaa aatcaatccc gttctgtgaa cagcccacca
    52441 ctgcaaccgg ttcggtgata gattcgtgct ttacatgcgg tacagcaacc cccttgccta
    52501 tgccggtgct ggcttcattt tcacgtttga tgactgcctt ggccagcatt ttggggtcgg
    52561 caccctgcaa tttcttatgc ttgctcaatg aagcaacaag ttcctggatc actttgtcgc
    52621 gttctgtgga ttccaattga gcaacaatcg catccaaaca aacaatatcg gctaatttca
    52681 tactgtctcc ttggtacaaa aaccactgtt gggattacca aagcatcttc cttgtcgcag
    52741 cataatccta ttagaccaca tcttcttccg acagcggcag agttttaatt cccgccggcg
    52801 gcatcgcctt atgttccctt tgtttttctt tgatttttcg aagctgcctt tccagcttat
    52861 gaactgccat atcaatacag gtataggtat ccatgccgat ttctttggca accagcggat
    52921 tgttgtgttc ggcatgggct atcacctcaa cgctttgttt gcctccctca ctgccttcta
    52981 taataacctc gatgcgactg ataccgtcat aatagcgagg cattttatct actttttctt
    53041 gaatatgggc gcgaatcgta tccgtaattt cgatatgctt tcctgttact gttacgagca
    53101 tcgggacctc cgattataaa gttatttatt cattttccaa aaccggttct gcacaaggcg
    53161 ctccgttggg ccgttgttca gccggcgcaa taactccagc gcttacaaca aaacggattg
    53221 cttcctctat cgatatatcc aactccaccg tgtcggtctt gggcaccata ataacatatc
    53281 cggtaaatgg tgttggtgag gttggaacga aaacggtaag cagttctgct tgaaccgtct
    53341 gtgccacttt ttttagacct ctgcctgtta ccagaccaat cgaccatatt cctttccgcg
    53401 gatactcaaa tgccactaca ctgctgaatt caagaccttt tttggtcagc agaaaatccg
    53461 ttacttgctt aatatatggg taaacttggc gtatcagcgg gactttcatc agggtttttt
    53521 ctatataatg ccatattgta tggcccatta cactggccaa gaaagcccct acaaaacaga
    53581 ccccaataat ggctatgaaa aaacccgtta ttttgccctg acccttgacc cagaaactgg
    53641 tcaggaattc ataatcccac cggtccgaaa ataatagtat taaacggaca agccccctgt
    53701 taatatgaat acttattttg tcctgaataa agatgtaaca ctggacaaaa atccatattg
    53761 tcaggatagt cggcaataaa gcagctacgc ctcgaaagaa gtagctgcga aaattcttca
    53821 acgcctgcat agcgcttact gtagtccccg gcatcgtgcc gtttataata tcattatcac
    53881 tgttttatgt gatacaaatc tactttttaa gtctaaacac taacgggcat tatgtacccc
    53941 gcagccgatt tcgtcaataa aaatataagg aagccgccga ttgtttagta aaagttggag
    54001 ttttacagaa ctttatacct cgataaacat cagattcatt gcaggtgaga aatagaccgg
    54061 ttaacaaaga ctccccacac tgatggtcac tgcttgtttc ctacgtgatg aggacccaat
    54121 agatgcacta tcatccccgg caaagaggca gcgatccatg ttatccgctg acacaatgcc
    54181 aacgcagtaa cggattctaa gtctgcatgg gcaaaggtta caaacaaaaa taccaccccc
    54241 ccttccagaa ttccaatacc ggctatgctg atagggatag aaccgataat ccatactatc
    54301 gggaaaaaaa caaaataata tccccaacag gcccctattc caagttcacg ccctatagcc
    54361 caatatgata aaattacaat actttgcaac aaaagtgtca aaccgaaact tacaacaaga
    54421 accagaggat aatgataata aatcgtaatt acttctttca gttgacccca aaaatataaa
    54481 actctctcga acagtctttt aaggatcacc tcaaaattaa tccaacgtcc taaaacaaaa
    54541 acagatataa ctattaggac aactaatata actgaataaa accggatatg agggtatctt
    54601 tccaataagg aacttattaa cgaatttgaa tggctgactt gcagcatgcc tttttggccg
    54661 cgcataaaaa gcaaataacc ggcaaccgca ataattaagg tagaaaccag acccataatt
    54721 ctgtcaatga tcacgccaag agcggcctga agttttttgg gactatgctg agatacatac
    54781 caagcacgaa tcaaatcacc gccgacagaa ctgggcataa agttattgaa aaataaaccc
    54841 ataaaagtca gcttaattgt caaccataga gaaatcgcca ctccttgtgc acgcagaaaa
    54901 atccaccacc ggagccccaa taatgcctga gaaaatatat agattaaact tgccgccggc
    54961 agcacaagag gatgcatgcg cttcaaagtc aagccaacct gccgaaaatc aacatcttta
    55021 aaaataatat agcacgcaac ggccgctata acgatgcgaa gcaacagtaa cagacgcggc
    55081 ttgagttttt ggttttcttt tgtcgtcaat agcaaacctc tggaaaattt cttgtttttc
    55141 aataagatag cgattaaaaa cagcaactgc aatagcagcg acggatattt tggataagaa
    55201 aatcgaaaat caggagtttt gtaaggtgag cataacaaat aaagaaattg aagaaggact
    55261 aagttttaag cccaaatttg acgcctctgg attaattacc gctatttctc aggatgccca
    55321 aaccggacaa atcctaatgg tcgcctatat gaatgaggag gcactcaagc tgaccctcca
    55381 gacaggcaac gctgtcttct acagccggtc gcgaaagaaa ttatggaaaa aaggagaaga
    55441 atccggccac gtccagaaag tgcgccagat acttgtggac tgcgaccaag attgcctgat
    55501 attaaaagtt actgtcgatc aaggccaatg ccatgtcggc taccaatcct gcttctatcg
    55561 ccaggtcgtc agtgataaaa cactggaatt catatccgaa aaaacgtatg accccaaaca
    55621 ggtttataaa aaataatctt tctctgtgaa aattgtataa agatttgaat tttataaatt
    55681 tatagatgcc aaaaacacaa aggtttctaa ttcgcttaga aaaaacataa aaatttggtt
    55741 gacagaattc aaataagttt gctaaaagga agatgctcct tctgccgcct gtggtaagcc
    55801 ctgggaagta caccgacttc ctcctcctta agccacaggc ggattttttt ttgcataaaa
    55861 aaccgtaatt aaatcaccac aaggagacca aataaatctt gatttttccc gattcctgct
    55921 ggtcaataaa tccttaaata actgtctttt aggcttttat tgtggtttga ttttctcgat
    55981 aaaataaaga atttattgag ttttccaagc aataagatat acttcaagct gattatatta
    56041 acgtttggtt tttttatagt tgttctttct atcatatttg aaaggagtta tttatgctgt
    56101 tatatgtttt gcgattttta ttctttatca cctcaacggc ttttctattg gtgggactca
    56161 atcgtaccgc taaaacagac caagatattt acatctttta tgcagctctg atactgacag
    56221 tcgttttcat agccgtcgaa tggcttacgc ctaaaaaagc actcttcaca ctaacaggag
    56281 tcttttttgg cctgctggtc ggcatggtta tcaactgggc tgtaaactgg ataatcatga
    56341 tgatgaatga cctgttctgg cagctgccgc ccaataccct tcgcatggga acatggtttg
    56401 cagatttgtg tatatgtttt ttatgcatca gtattgtgat ccgaaccaag gacgatttcc
    56461 gttttttagt gccttatgtt gagttcactc ggcaaactcg cggtttgcgg cctttggtac
    56521 tggatacatc tgttatcatt gacggccgca ttgcagacat tgcctagacc aagctttttg
    56581 acgccccgct gattgtgccc cgatttgtgc tcaatgaact gcagcttatt gcggactcgc
    56641 cggataaaat caaacgcaat cggggccggc gcgggttgga tatgctcaat aaactccagc
    56701 atagtcctgt cgtagaaatt acggttgacg atactccccc gcccggactt gacgacaaaa
    56761 gcgaagttga ccagaaattg gttgctttta cgaaaaactg cgatgggcgt ctggttacta
    56821 acgactttaa tctcaacaag gttgcatccc tgcgcggggt ggatgtcata aacatcaatg
    56881 atttggcaaa tgcccttaaa acagttactt tgccgggtga aaccatgtcc gtcaagatac
    56941 tccgtgtagg agaagatcct catcagggag ttggatatct tgaagacggc acaatggttg
    57001 tagttgaagg tgccaatgat aagattgggc aaaccgttac ttttgccgtg accagtgcgc
    57061 tgcaaacctc cgccggccgg atgattttcg gcaaatgcga caatgccggc ggccccgcaa
    57121 ccaatgagaa tgccggtttg gagcgaaatg gttcgtatga ctccagaaaa cggcgacggt
    57181 cttatccaaa tacataatga atcctgtccc caatcatcga aagccgccct cgtggctcgt
    57241 tgacagacgg aatacagaca gcctgaaatg gcgaaagtat cagaatacgg atatcctgcc
    57301 gctgtgggta gcagatatgg actttcgatg tgctgaaccg attattgaag ctcttcataa
    57361 acgggttcag gagggcattt tcggctatgg cgctgttcct ccggccgctg ttgaggcagt
    57421 gatgggctgg cttgaaaagc agcatcagtg gcacatcgag ccggactgga ttgtatgggt
    57481 gccggg
//
LOCUS       DAOTBO010000005        55501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_476_length_55501_cov_5.576345, whole genome shotgun sequence.
ACCESSION   DAOTBO010000005 DAOTBO010000000
VERSION     DAOTBO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 55501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55501
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_476_length_55501_cov_5.576345"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLE88_01195"
     CDS             <1..474
                     /locus_tag="PLE88_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOM75113.1"
                     /translation="KELRIRHAVRMAFFFGGFQALMPLLGYAAGSTLTGFVNRWDHWL
                     AFGLLTAIGSKMIYEAAKIEAVEKRPQDPSNIAILFALSIATSIDALAVGVTLSLVTT
                     HIAEAVLLIGLITFGMSYTGYELGKRIGHFFENKIEILGGLILIIIGLKILLTHL"
     gene            580..1440
                     /gene="larE"
                     /locus_tag="PLE88_01200"
     CDS             580..1440
                     /gene="larE"
                     /locus_tag="PLE88_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOM75114.1"
                     /translation="MDIQKKYQTLQKILKEYGKAAVAFSGGVDSSFLLKACAETLGRH
                     NVLACIGISPSLSGYQLQQARHLAELIGVRLIEVRLHELDDPNYTANRADRCFHCKSC
                     QFRMLGQAAAAEGFRHLLCGSNYDDQDDYRPGNRAAQMLGIASPLMQAALTKSDIRQL
                     SRQLGLPTAEEPASPCLASRIAYGQPITEAKLAQVEQAEAFLRSLGFREFRVRHHGTV
                     ARLEIPPQEIEKAAGPELRKHITEELKKLGFCYIALDLEGFRSGSLNEMLTDAEKNTE
                     QQPPNWSNRQ"
     gene            complement(1418..2725)
                     /locus_tag="PLE88_01205"
     CDS             complement(1418..2725)
                     /locus_tag="PLE88_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75115.1"
                     /translation="MEAIIRQAIDFLCAYEMDHQDIDFDDHIKRFLREMEAGLAGLPS
                     SLEMIPTYLQAVNQIPVGRRVIAADAGGTNVRVAAVYFDENKKPVIENLRTFSMPAVE
                     SGLSRIAFFDAMAGYFAGVLDASEKIGFCFSYPTEITANRDGRLIRFAKEIKAPEVVG
                     QMVGENLNLAIGRLGLGGSKHIVLLNDTVAALLSGVGFQNRLYSSYIGFILGTGTNCA
                     YIESNANIAKQKGLDPRQSQIINTESGGMAKAHWGQIDRQFDASTTNPGVHFFEKMIS
                     GAYLGPLFLTVLHRACRDGLLSGGAAKAIGQLSDLSTKQMNAFMLYPCGSNPLAGALA
                     GGTSQDAVLAYVIADRLVERAAKLAAVNLSAMAVKCGQGTNPACPICIVAEGTTFYQM
                     KTLKSRVEFYLKQHLENQRGIYTEIIGVEHAALIGAAIAGLTN"
     gene            complement(3117..4100)
                     /gene="galE"
                     /locus_tag="PLE88_01210"
     CDS             complement(3117..4100)
                     /gene="galE"
                     /locus_tag="PLE88_01210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOM75116.1"
                     /translation="MRILVCGGAGYIGSNMTALLAREGHHPVVFDNLSKGHRSAVQNA
                     QFVLGDFGDFNVILETLQKYRIEAVMHFAAFIEVGESVQMPLRYYENNVSKTRTLLDA
                     MEKAGVQKFVFSSTAAVYGMPAGVPITENMPKEPINPYGETKWAVERMCHFQAAAGRL
                     RYAALRYFNACGAGFNAALGEDHRPESHLIPLIIQAAMGKRDSIKIYGTDYPTPDGTC
                     IRDYIHIEDLCKAHLLALKRLDDQPELAYNLGNGRGYSVRQVIDTVRKVSGRDFKVIE
                     ADRRPGDPPVLTADASKARRELGWTTDFPELEIIVESAWKFHTRHPNGYES"
     gene            complement(4097..4672)
                     /locus_tag="PLE88_01215"
     CDS             complement(4097..4672)
                     /locus_tag="PLE88_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75117.1"
                     /translation="MRTSLVASLLLLAAGLIGGCAGSEKGASSAPLCVSDCSAELAMQ
                     AAQKVLTKMHFEIEKYDYDARYIRTRPLTGGQFFEIWRQDNASASAAAQANLHSLRRI
                     VEMDFDQKGREVCIGCRVQVLRLSVPEQPLRGMGLMAEGITESSRSVQTLTVDPALES
                     RIEWLDAGSDAKLEQRIVKRIERVIRRNAAQ"
     gene            4815..5753
                     /locus_tag="PLE88_01220"
     CDS             4815..5753
                     /locus_tag="PLE88_01220"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOM75118.1"
                     /translation="MLINQLQLLRNKLKNLPLGGVIEAFLDYLSVEAGLSDNTLLAYG
                     RDLMGFAEHCQRQKIADMDKILPVTVYSHLRQLAQNGQAETSISRNLVAIKMLLRFGV
                     LTGRISQDISGTLEGPKVWKRLPGIASKEQVFRLLDAPCPEDPYYWRDKAVLQLLYAT
                     GARASEVAALKIQDVNLEIGYVRCFGKGRKERIVPFGRTAAATISDYLKQLRPLLAKP
                     HSPDRLFLSRTGRGLDRIELWRIVKKYAVRAGMPKNLTVHTLRHCFATHLLSGGVDLR
                     ALQEMLGHADIKTTQIYTHVDQDRLRTIHKKYHPRA"
     gene            complement(5791..8031)
                     /locus_tag="PLE88_01225"
     CDS             complement(5791..8031)
                     /locus_tag="PLE88_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOM75119.1"
                     /translation="MRWLMSTLIMGIASIHPMAAAADSLAGPPANKLCLWYRQPAADW
                     MTSALPIGNGLLGAMIFGGAKEEHIQFNDKTLWTGSRTSRGAYQNFGDVYLRFDHPDS
                     VSLYRRELDLEEGIARVCYTVDSTDFSREYFASYPDKLIVMRITAGKPGAISFALTLQ
                     DAHSGTKNTAPDAISISGKLTVLSYLASVKVIPEGGRLDCSSEQITVAGADAVTILLA
                     GGTDYDPTRAEYLGSGLQERIVNCIKEASAKSYAQLKSRHLADYQALFNRVCLSLNDS
                     KPAIPTDELLTSYNAGSYDPALDVLYFQYGRYLMISCSRGMALPSNLQGLWNNSNRPP
                     WECDIHSNINVQMNYWPAETANLSECHLPFLDYIYNEAAVQTSWSNMAASLGCRGWTM
                     KTQNNIFGYSDWNWNRPANAWYCMHLWQHYAYTLDSGFLSQKAYPVMKSACEFWLDRL
                     TADADGTLAAPDEWSPEHGPWQTGVAYAQQLIWDLFTHTVKAAEILKTDAEFRALLQE
                     KLSRLDTGVRIGSWGQLREWKYADDNPQDTHRHISHLIGLYPGSQISPLMNPDDAQAA
                     RVSLNARGDGGTGWSRAWKICAWARLLDGNRAHTLLKNALRLTSNTRLDMSDGGGVYE
                     NLLDAHPPFQIDGNFGAAAGIAEMLLQSHLGFIDLLPALPDAWPSGRITGLRAQNAFE
                     ADIEWKDGRLTRAVIKSDRGHPCRLRGNYSVTLRNGTAVKTVYKDGFIAFDTHSDEQY
                     ILTPLQ"
     gene            complement(8147..9130)
                     /locus_tag="PLE88_01230"
     CDS             complement(8147..9130)
                     /locus_tag="PLE88_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOM75120.1"
                     /translation="MNQNDIRRLSLAGLAVLSFFIPTMPARTPSEQVPLPKIEPGVVK
                     VMTFNIRNDTIVDGPNRWSRRKNIVFNMLRDHNADVIGLQEAEDEQVCDIQMALPCYS
                     RYAVGRNDGRRQGETCAIFYRTDRFRLLDSGTFWFSDTPSVPGTKDWGNLWPRICSWV
                     RLAERQSGQAFYVYNVHLDNLSQHSRQKSVELLARQVASRKSRDPFIVMGDFNMELDN
                     PAMRYLQKQGIQTPYPRMVDAWSSVHSGKPSLGTRHSFRGSISGPAIDHIPICETLQA
                     LDVSIDRRAGENGRYPSDHFPVIARILLKPSALPEYAALSSSAASSLDRIP"
     gene            complement(9383..10285)
                     /locus_tag="PLE88_01235"
     CDS             complement(9383..10285)
                     /locus_tag="PLE88_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOM75121.1"
                     /translation="MKRFLAVTALLGILLAGCVTENKAAGRKIVFPADDPNIINAVTF
                     NIRYGTADDGPNSWANRKELVFNLLADQAADIIGLQEALDFQVNEIRAALPQYSVVAV
                     GRDDGRKQGESCPIFYRRDRFSRLDSGTFWFSNTPWIAGSKHWGNTLPRICTWICLKE
                     KKTGKLFYVYNLHLDHQSQLSRQKSVELLAKQIARRPHGVPFIVMGDFNIGLSEPAMA
                     YLLAGSPLSNQIRDTWQYLHPDQTPPGTYHAFEGTARGPKIDHILIGPGIEISEVAID
                     ARSFNGRWPSDHFLVAACLNLTSK"
     gene            complement(10475..12175)
                     /locus_tag="PLE88_01240"
     CDS             complement(10475..12175)
                     /locus_tag="PLE88_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75122.1"
                     /translation="MRNAAFISIFTLSMWMSVYCLSDTYIWTSSAGGQLGDPSNWLPF
                     GIPGQQDDVRFTLAGSYGVWLDQPYTHQTLMVDGSELALDLRGHNWRFEGWDDRYRCV
                     TIGDANASKLAVSGGGTVSSHAVFLGWGNDLETVLELAGENTEWIANIDGNWDGLFAG
                     GVGGLARLRVYDNAVLKHGHGESGRFRGNQTVIEIEDPNSEWYVDGWFGMSWEGATDV
                     FLKNGARAKFGMLEMAVLPGSSAVISLTGRETELAIENWWDYVPLFIGRQGWGVVNMT
                     DSRLFCSGNMVLAEQPGSAGQLNLYGSSWADCQGSAAVGGDLDSRGGTAMIHLQGDLW
                     NGFYSTLTAAYSYEGQSIVVWPKGTIRMDGGTITLEYGDRANPVVLKGGILEGWGWIW
                     GDVQNESGRVALGAIGDWKALDIGYNYTQNAAGVLKISIGGRDTYWQYGGLVLTNGWG
                     EYGRAYLDGMLEVELLYGFIPDPSDEFVIIDAQQVEGQFVNAAGAGGIGKYVFEQGTF
                     DVWYESNRVVLRNFRTEPSCPAFPDADLNRDCRVDLADLSRMAAQWLDCGLQPLDYCY
                     "
     gene            complement(12340..12960)
                     /locus_tag="PLE88_01245"
     CDS             complement(12340..12960)
                     /locus_tag="PLE88_01245"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOM75123.1"
                     /translation="MSNQRLSMAYLPKSQFILASASPRRKLLLQKAGYCFEVVPSNVD
                     ESLYDPQGLTSDIYAQQLALAKAKDVAARFTNALVVGADTVVDLDGEIIGKPDDAAHA
                     EAITRKLFSKPHKVITALALVCIEKTIEIVETDTTIVYPRLLTEAQIAEHIRKGDWQG
                     KAGAYGIQESGDAFVERIDGSFTNVMGLPMERFGILLQKLGITPPK"
     gene            complement(13022..14095)
                     /locus_tag="PLE88_01250"
     CDS             complement(13022..14095)
                     /locus_tag="PLE88_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75124.1"
                     /translation="MRFNSSPTVVKARCLCGLLCAAGLLFGSGCRQTGVASPHLSACL
                     SDSPQSIAAYLDDEPAIRSVQVWHSEEGQGLVVRTDHYVLYTTLLEPLMLRQIPAFLE
                     AACSACQSQLPQPLSISRPMEVYLFSSRSQWEQFTHRFAGSDAAIYLRIQRGAYTLRG
                     VIAAYNIGRKQTFSVLGHEVWHQFNQQTFAYRLPSWLDEGIAALFETCRYTQGRFVFE
                     PQYNLMRLGALKETVQRSSLISLPSLIILNPGQVLDSSNDAVAAFYAQNYALVRFLRE
                     YNYGIYLRRYHALLFGAVDGTWPLPPEAAALAADRLRPLTVGWNMQVSPALFNFYIGD
                     VSTLDADYKDFCRKIVYAVRLKD"
     gene            complement(14082..14648)
                     /gene="amrA"
                     /locus_tag="PLE88_01255"
     CDS             complement(14082..14648)
                     /gene="amrA"
                     /locus_tag="PLE88_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOM75125.1"
                     /translation="MNENQKKILLKTAKQTIDAAVHHRPAPVPHTEDPVLLEKQGCFV
                     TIKNRDQLRGCIGQFTADRPLIEMVSQMAYSSCMRDPRFAGDRIRASELPELDIEISV
                     LSSLEKTDDPLSLRLGIDGIYITDGLRCGCFLPQVATETGWSAEEFLSYCCAHKAGLP
                     PDAWKTDPRVETYLFSADVFGAGWNEIQ"
     gene            complement(14661..15296)
                     /locus_tag="PLE88_01260"
     CDS             complement(14661..15296)
                     /locus_tag="PLE88_01260"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOM75126.1"
                     /translation="MSYTVQAAPAWWHWHRRLYNWVIHFADTPYGERALFLLSFAESS
                     FFPVPPDVLLAPLTLGAPKKWLRFAVSCSIASVLGGIVGYLIGLFLWEQIGQWVFAHL
                     GVIGFTQTNFDKFQAGYQKYDFWIVFTCGFTPLPYKVCTITAGIARIMFPGFLLASAL
                     SRSARFFLVAGLFGWKGEQIRPFIDKYFNWLSLVFVMLLIGGFAVLKLLHH"
     gene            15348..16289
                     /gene="ispE"
                     /locus_tag="PLE88_01265"
     CDS             15348..16289
                     /gene="ispE"
                     /locus_tag="PLE88_01265"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOM75127.1"
                     /translation="MQKQESQEIPNSPIMTYLNQFETAGNGLLVRAPAKINLSLLIKG
                     KRPDGYHELETLMAKINWYDQLLFEPGKSAGIELLCQGPCWAPEGEDNLVYRACRRLL
                     EETKTDIPIRVTLTKNIPAGTGLGSASSDAAAALLGLNRFGGLNQKKDILDRLAAELG
                     SDVNFFLGGPLAFCTGRGEKIEEIQPPTDFCALLLIPNITTSTKRVYENYRHSDKDYL
                     RFRGIINPLLAKKSIDSIAKICANMLESSCFELHPQLQQIKEVCQQQLGLKACLSGSG
                     SAMYLLMDEPCAGLERLQDHLKKEYSCESRIVNNNRW"
     gene            16311..16766
                     /locus_tag="PLE88_01270"
     CDS             16311..16766
                     /locus_tag="PLE88_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOM75128.1"
                     /translation="MEISEVRVKIVANQDDRLKAFCSMTLDNEFVIRDIKIIEGTGGL
                     FVAMPSRKMSDHCPKCGGKNYLRAKFCSNCGTKLPENRLKQDPQGRIKLHADIAHPIN
                     VACRKKIQDAVIKAFREELERSKQPGYKPVELDEDDDYPLDDHHSESVS"
     gene            complement(16850..17155)
                     /locus_tag="PLE88_01275"
     CDS             complement(16850..17155)
                     /locus_tag="PLE88_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75129.1"
                     /translation="MKTSKHTQNEHPKERDSIRLLQDRIDSAVSQQDVAALQEELLES
                     MQSNPLGRLLKVIASLPEVRSEKIERARRHIEQNDDCWDTKMDLVLDKVLEELINEE"
     gene            complement(17680..19536)
                     /locus_tag="PLE88_01280"
     CDS             complement(17680..19536)
                     /locus_tag="PLE88_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOM75130.1"
                     /translation="MKKDMSLRVVLRVFLIAAGLLSLSCRTASEISADQSLSLWDQPA
                     RQLLAKMTLDEKIGQMTQADHEFIKDPADVEKYFLGSVLNGGSSDPAEGNSPKAWADM
                     YDRYQQHALKTRLGIPLLYGVDAVHGHNNVIGAVIFPHNIGLGCTRDPQLVEEIARIT
                     AVEMRATGVNWAFAPCVTVPQDERWGRTYEGFSEDPQLVSLLGQAAIRGLQAGRLGDP
                     LAVAACAKHYAGDGGTAFGSTKDGRGLDQGDTRVDETTFRRIHLAPYIPAVKAGVGTI
                     MPSYSSWNGVKCSANKYLLTDVLKKELGFEGFLISDYNAIDQIDPDYKTAIMISVNAG
                     MDMAMVPQRYPEFIHCLKELVNEGKVPMERIDDAVLRILRVKFAMGLMDKNRSVLTDR
                     TLMARLGSEDHRQVARKAAAKSLVLLKNDNKILPLSKKIARIHVAGKNADDLGSQCGG
                     WTIDWQGRTGQVTEGGTTILNAIRQAVSTNTQVTFSKDGTGADGADVGIAVIGETPYA
                     EMKGDRDNLNLDSQDIETVEKMKKAGIPVVVILISGRPMIITSILDKADAFIAAWLPG
                     TEGQGVADVVFGNQKPSGKLSYSWPRDNSQIPVNIGDSDYNPLFPYGFGLTY"
     gene            complement(19669..21015)
                     /gene="thrC"
                     /locus_tag="PLE88_01285"
     CDS             complement(19669..21015)
                     /gene="thrC"
                     /locus_tag="PLE88_01285"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOM75131.1"
                     /translation="MKNKDRAYQKCINLQCSAEFDCSQVLFKCPRCGDLLDVKYDWNR
                     ISLPARLRDFSKRWASRQNRLDFSGVWRFRELLDFCDDPYKVTVGEGQTLLQQNDALA
                     GQLGMGSGTLFLQYEGLNPSGSFKDNGMAAAFSHAKMVGAVASACASTGNTSASMALY
                     AHSSGLKATVFIGEGKIAFGKLSQSMDYGAHTIQVAGDFDDCMRQVQDVCAQLHIYLV
                     NSLNPFRLEGQKTIMLRIIEGLNWEVPDWIIVPGGNLGNSSAFGKAFAELKEVGLIDR
                     IPRLAVINARGANTLAELVNDKGLRWNGGHVNQAIADAYFAGLDAAGYRPNTIASAIE
                     ISRPVNLKKCLRALEVCNGVVLDVTDEEILDAKAQVGKFGLGCEPASAATIAGLKYLL
                     ADGTVDKNARVACVLTGHLLKDPNATVNYHSKMARIFSNPPVQVKNDLDEIIALMK"
     gene            complement(21087..21878)
                     /locus_tag="PLE88_01290"
     CDS             complement(21087..21878)
                     /locus_tag="PLE88_01290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM75132.1"
                     /translation="MVSRQIQAVLFDLGETLLNFGRLDRAALFEEAVNRSYRYLIELA
                     QPVGSLRAYRRVHIWGIRWHLLKSWLTGDDFNSLELLKAYGRKRGFTLSEQQWQELNW
                     QWYVGLSRKGAVEKETAPTLRCLQHMGLKLGLLSNTFVHKSSLERHLQQEGLLDLLPV
                     RLYSYEFPFRKPDVRIFSVAAERMGIPADRTVYVGDRMDNDVKGALAAGMLPILKKAY
                     TNRNKPVPADIACIETIAELPGLIKKMIRPDNGISRQEQPTYAVH"
     gene            complement(21882..23204)
                     /locus_tag="PLE88_01295"
     CDS             complement(21882..23204)
                     /locus_tag="PLE88_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75133.1"
                     /translation="MTQKIQLQDVDRPNLYRDVFPYTEFPKVFFENETVAMQIPGKVW
                     ITDTTFRDGQQARPPYSPDQVLRIYDYLHQINGGTDLIRQCEFFLYSEKDRKAVELCR
                     QRGYDFPEITGWIRAVAADFKYVTQLGLKETGILTSASDYHIFLKLKKTRSQAMEAYL
                     GIVKAALDNGILPRCHFEDITRADYDGFVLPFADKLLELSRQYNVPVKMRLCDTLGFA
                     LPYPNSTLPRSLPKLIAGLRRIGVPAEWLEWHGHNDFHKVEINAATAWLYGCCAANCA
                     IFGVGERTGNPLEALVVEHAQLFGTNPKVNYQAITELADYANKELGFNLPHNYPLVGR
                     DFNVTRAGIHADGLLKNEEIYNCFDTQALLKRPVGVAITDKSGAAGIKHWIEERYAIE
                     IPKQDPRIIAIKDRIDAEYAADRMSAISDEEMIAWTEEIFGDKLPPLR"
     gene            complement(23295..23378)
                     /locus_tag="PLE88_01300"
     tRNA            complement(23295..23378)
                     /locus_tag="PLE88_01300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23343..23345),aa:Leu,seq:taa)
     gene            complement(24773..26092)
                     /locus_tag="PLE88_01305"
     CDS             complement(24773..26092)
                     /locus_tag="PLE88_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM75134.1"
                     /translation="MNQFGRISLIPVGFVLFLIQIGIADNPLILDQFTADPTARVFEG
                     KIYVYPSHDIPVPPGSGARQDWFCMEDYHVFSSENLIDWTDHGVILTQSEVPWLTRMS
                     YSMWAPDCVFRDGKYYFYFPTGGRIGVAIADNPYGPFKPEPTFIQGIRGIDPGVIIDK
                     DGIAYLFYSDLMVAKLKENMVEIEGPPQQIANLPKKGLREGPFPFVRNGIYYLTYPHV
                     QNNTERIEYAMASSPMGPYEWKGVIMDESASGCWTNHHSILEYKGQWYLFYHDMDLSP
                     NFDKNRAIKADYLYFNEDGTIQKVIPTVRGIGNAPATNMIQIDRYSAKSENGAAVEYL
                     NPEKTFDGWKTVLSAPDAWIRYDRVDFAGKTVKTLKVRAMSATGGSIEVQIGKDDAAI
                     KVPVVIAKCTDWTIASSNVSAAPSGLQDIKVVLKGDDRVEIDWIQFE"
     gene            complement(26151..26777)
                     /locus_tag="PLE88_01310"
     CDS             complement(26151..26777)
                     /locus_tag="PLE88_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75135.1"
                     /translation="MASLYYDGNAKRWRVAWRCTLPTGEIDRGSKSFGKDKKTALEFK
                     KHCDENEKRLKRTVFVAPVFLSDVVEEWKEYCQRYTPCTRDLYIFSEEHFIECLPESV
                     NYITDLTNAHINRHINSEMSRGLKNRTLNNVLFSVKNLCRYMQENYKIPNPCRGIKKM
                     REDPPDHNFLNEEEYQTVLPNTKAIAKPWVVFLEKAVTKHWNVKMALE"
     gene            complement(26988..27070)
                     /locus_tag="PLE88_01315"
     tRNA            complement(26988..27070)
                     /locus_tag="PLE88_01315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27034..27036),aa:Leu,seq:tag)
     gene            complement(27323..27619)
                     /locus_tag="PLE88_01320"
     CDS             complement(27323..27619)
                     /locus_tag="PLE88_01320"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOM75136.1"
                     /translation="MAKKQEKGDIDKLSFEQAIDALTEIVDKIEDGQIPLAESLQQYE
                     KGMALIKHCRKILLDAEKRIENISKDKSDIQIAADLEEESSNEDSEMSAEEDLF"
     gene            complement(27626..28999)
                     /gene="xseA"
                     /locus_tag="PLE88_01325"
     CDS             complement(27626..28999)
                     /gene="xseA"
                     /locus_tag="PLE88_01325"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOM75137.1"
                     /translation="MANIKPQLPIFTVTQLNTMIKAALEEALPPRLMLRGEISDFKQH
                     TSGHCYFLLKDAGGQIPCTMWASHFRQVKFRCENGLAVLAAGHIDVYLPGGKYQFYCD
                     KLEPAGMGALQLAFEQMRRRLEAEGLFDPARKKPLPRYPMNIGIVTSFSGAALHDIAD
                     SIQTRWPCARLYVFDTPVQGEGAAPCIAAAIAKANRLRKQLGLELLIVGRGGGSMEDL
                     WAFNEEAVARAIYASKLPVISAVGHEVDVTIADLVADKRVSTPTRAGVEAVPDWREEL
                     QKIQSLARRLQNTIRSQVQNSRNLLAAVLASRVFRLPLGPVQIAAQRIDELANRLMHS
                     VQRHLAYRKDQLERMRAMVQRIEPIRLLAKNQLAIQTLEGHSRTAMLQILNKNQLQLA
                     ALENRLQALNPRSVLKRGYSITINQRTGCLVTDIKQVLINDPLTTELAAGRIISRVEE
                     TKNKPLE"
     gene            complement(29302..30153)
                     /locus_tag="PLE88_01330"
     CDS             complement(29302..30153)
                     /locus_tag="PLE88_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOM75138.1"
                     /translation="MKINVLCIGDIVGRPGRRILAQQLFSFVRDNSIDCVIANAENAA
                     GGSGLTPQIYDKLLKYGVHLITLGDHCFRKKDIIPTLETQNNIVRPANLSPSAVGREY
                     AVYQTVKGPSVAVVSLIGRIYMKPADCPFAKIDTLLPRLKSEADILFVEIHAEATSEK
                     IALGYYLNGKVSCVFGTHTHVPTADERILTGGTAYISDIGMTGAADSVLGRNPESVIR
                     AFRTQMPYPFDVAAGDVRISGIVVTVDSHTKKAERIERIVLHDQAAPDTLTYDSDDGR
                     PDYTNGF"
     gene            complement(30204..31787)
                     /gene="rny"
                     /locus_tag="PLE88_01335"
     CDS             complement(30204..31787)
                     /gene="rny"
                     /locus_tag="PLE88_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOM75139.1"
                     /translation="MEILAQMSVATSIFIGVVIGILAGGGIVFVVLQGIIKTRAKVTQ
                     EEIEARIETAKKQAETILKEARLDAAGEIMKKRQEFSAEVSSRENQLRMQELELTKKQ
                     DLLERQQEKIQQQEKQLANKDRELERQINTYNNRNKELANLVAQQKNMLLKITSLDVE
                     QAKEMLLSRLEDECEREMNQLIQRKTAQAEEMAEEKAKEIINLAIQRYAAEQTCEVSV
                     SMVDIPSDDMKGRVIGREGRNIRAFEKATGVDVIVDDTPGVIVVSGFNPIRREVARLS
                     MERLIQDGRIHPSRIEEIVSQAKKDVHSRVIQLGKEAAEEVDVRGLNNKIIGMLGALH
                     YRTSYGQNVLRHSVEVAFLAQVMADELGLDGSLAKRAGLLHDIGKAMDREIEGGHPAI
                     GANFLRRFKESNVILNAVEAHHGDIPADNPYTPLVAAADAISASRPGARRETLERYIK
                     RLEKLEEIAAGFEGVENCYAIQAGREIRVIVDAEKVSDDVAMKTARDIAKKIEDEMTY
                     PGEIKVTLLREVRCVEYAR"
     gene            32573..33148
                     /locus_tag="PLE88_01340"
     CDS             32573..33148
                     /locus_tag="PLE88_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM75140.1"
                     /translation="MRRKDVLTTGQVAQLCKVAPRTVTKWFDSGQLRGYRIPGSKDRR
                     IPLSELIRFMKEHDIPTTELEKGKIRILIIDSRTDYSQKLAEELKMRGSFEVQCAYNS
                     FDAGLMSLKFSPNIILIDLMNSSIDARGLCNDVRSREELSDCMLIALAGGLGEKEAQN
                     LHKQGFDAVVTSTNNINAILACIQKNFTILY"
     gene            complement(33142..34302)
                     /gene="dprA"
                     /locus_tag="PLE88_01345"
     CDS             complement(33142..34302)
                     /gene="dprA"
                     /locus_tag="PLE88_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOM75141.1"
                     /translation="MVQHHSADIEKWLKLIRSDGIGPVLFKRLLDAFGDVDRILAASA
                     ARLAEVKGISQTTAQQIARTRNTFNAEKEIEHADKLGVWIVHLHDPRYPPALKAIYDP
                     PPVLYVKGTLTRADTLALAIVGSRRCTLYGTEQAGRFAHLLASAGFTIVSGGARGIDA
                     AAHRGALSAKGRTIVVQGSGLANPFPAENQKLFEQAAESGAVISELPLTYEPLAANFP
                     GRNRIIAGLSLGVLVVEAAHRSGALISAQAALDNNREVMAVPGRIDSPASAGCHQLIK
                     QGARLVDSIDDIMAALGNVGDGLQDYAASAAGQAEQNAQKSLFDVSRLNLTKEENAIL
                     DCLNSEPVHVEDLIAQTKLTAGQVHAAAVSLQLKGLIRQLPGGMFVKRHQTQ"
     gene            complement(34490..36793)
                     /locus_tag="PLE88_01350"
     CDS             complement(34490..36793)
                     /locus_tag="PLE88_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOM75142.1"
                     /translation="MDDAAKMRQSYPCSVLCGLLMFTTVFAAESVLPDTEKLFDMSLS
                     ELMDVEVYVPATLTETNPLKTPASVTIITARDIALTPARNLLDLIEIYVPGAIYMNHS
                     VGPVPGIRGVIADRPYKFLVNINGININIKSHYGARLELLNWDLNDIERIEIVRGPGS
                     VTYGPGAIGGVINIYTKQAAQHPGLAIGGAFWDRYDSIQNYLSWGWSTEAFKVYSYVS
                     VADTAGHTPDLFSIDKSHSGYLGQPDGPYGPNPPATYMSDYDNQPQIKALIDVQLKDT
                     WRFWARYASSSSSLVQNSAIKYPFGNEWEDFRQTRFRYFQFAAENNTALNEEFALKST
                     FGISSIDVHNVEKYDGSIVNDRDNLQNIGWIWSEWEYFARFMLNYTAPDEILKAGIGV
                     EASYDLVRPAWGKGKDDGLRLSDGIISGPSSEAYGTGYRQLLYTNEKYFAVGHGWETA
                     SHAFLGEVNIKHSPKMTTLWSARLDKHSYTDYLFSPRFAWIYELDSEQFLKFIAQRSV
                     RMNTQEELYMNHKLDEDNKPEKLDTFELIYTAKPTDHLTFQASGFYNKNEVIAWDWAL
                     RRSAPVGQLKTAGLELEGQYSKDNVQIGLNHSFLKQLDWELGENIAVSGISYSDYYLK
                     VSDSITITSKGNDLNNWPTNATKLYTNIGLYGGKMTFHADMRTLWGFEGSRIGLDALS
                     EAGGNAAQIRHIKEQDAYDFEITANLSLTYHINKSADLTFFVQNIPVIGDNKRYSYSS
                     GFKYAYPDKASWVEEPTVVGVRYQVRF"
     gene            37134..39155
                     /gene="pheT"
                     /locus_tag="PLE88_01355"
     CDS             37134..39155
                     /gene="pheT"
                     /locus_tag="PLE88_01355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOM75143.1"
                     /translation="MKISLEWLRDYIDIDLPAQAIADTLSNLGFPIESIEQVGQDTVL
                     DVEITSNRGDCLGHIGIARELAAATGQPLKIPAIKLQESPEPTCLKVQVEIREPTLCN
                     RYTARIIENVKVGPAPEWMRRRLEAVGIRSINNIVDVTNYVMMEAGQPTHAFDFSKIG
                     GQKIVVRKAVNGERLVSIDGTKCELDSSMLVIADADRPTAMAGVMGGLDSEVSEQTTT
                     ILLEAAHFEPVCIRRTARRLSLQSEASFRFERQVDTDNLAWVSNRCAQLMAELSGGRP
                     TQGIVDVYPRKAERDTIGMRPSRLRALLGIEIPQADVLSIFSGLGFAPEVRHPDLIVC
                     TPPSWRHDLWREADLIEEAARCWGYDKIPVESKIHIKVCRPNLREKTAGCVRTFLTGA
                     GFYEAVTVSFVNQKAAEVFSGRPAEEHLSVSDVFGKNTNLLRQNLIGSLALVFQSNRR
                     AGNKPCRLFELADTFIPQDNQPGQLPHEHLHIGLIMDDEFRAMRGVIEGLVQKICPTA
                     LLDFRPSSLAWASASAEILLNGKPFGWAGLFSPETAAQFDMDKEIITAAELDFEVLME
                     YSGSVPTVQPVPRFPAIVRDLSLIVDESVTWAQITAVVQSRAPEELEKIDFAGLYRGK
                     PIPDGKKSVTVSLRFRDEEGTLRHETVDGFQNEILGALIQTLGAELRTA"
     gene            39230..40321
                     /locus_tag="PLE88_01360"
     CDS             39230..40321
                     /locus_tag="PLE88_01360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOM75144.1"
                     /translation="MAKEVVLPQLGQSMREGTLTAIRVDAGQSVKAGDILFEIETDKA
                     TLEMESPEEGTVKAVLVEEGQTVPVYTPLLVLGDPDEEIDPAYLTQLEWKVQAALLNS
                     TPAQQQSAPAGLSAHTLRTAEAEQRCRPAEEIPPAALGQQIPLTRLQKIIAEKMVWSK
                     QHIPCFYLNVRVDATRLVELRRQLNAESSVKYSFNDFIIRALAVGIGHYPIMTGQLAG
                     DCIQLSDRIDIGLAVAIDEGSVAAPIIKDCGSKTLEEISRASRDLIDRAHANKLSLDD
                     LTGGCISVSNLGAYGVDSFIPIVIPGQTSILGIGSLQDMVIPVDNRPTTRTVMNLTLS
                     VDHKVVNGAEAAQFLDYVKKLLERPEELV"
     gene            complement(40322..41572)
                     /locus_tag="PLE88_01365"
     CDS             complement(40322..41572)
                     /locus_tag="PLE88_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM75145.1"
                     /translation="MINISKLYCGQLTPGDWLRYGIEGGQRAGDSVPLAASERKPIVV
                     WNITSRCNLRCVHCYSSSGTDAADCAATTQEAKTVLDDLAAYGVPSVLFSGGEPLMRA
                     DLFELIDYAVQKGLHAVLSTNGTLIDAQKARQIKNAGVSYVGISLDGIGESNDRFRGA
                     AGAFEKAVAGIRHCKDAGVRVGLRLTLTGRNIEDLEGLFHFFEKEGIERACFYHLVPA
                     GRGQRIYSDDLTAFQTRQAVEMILAKTRVLTQGGRKTDILTVDNHADGVFLYLKLLVQ
                     DPARADEVWKLLTWNGGGLASSGVGIGCIDFNGKVHPDQFWMHYDLGDIRQKPFSEIW
                     SNQQEPLLKGLRRRRDYVKGRCRLCRYFDACGGSLRVRADLYYGDPWAPDPACYLTDE
                     EIGLDEKKKQILARSGEIYHLPNE"
     gene            complement(41565..42623)
                     /locus_tag="PLE88_01370"
     CDS             complement(41565..42623)
                     /locus_tag="PLE88_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM75146.1"
                     /translation="MTKHAADYTRTLRLLFWESTARCNLSCIHCRRLDAEQTADLTTL
                     QAKEMMDQLAALGQAQAFMPLLVFSGGEPLCREDLFELLEYAAAKGIHRALATNGTLI
                     DAAAARKIKSAGVQRVSVSLDGADARIHNKLRRQDGSFEAAVCGIRHLRHNDVPFQIN
                     MTLTRHNAGHLDAVLELACALGAAAVHVFGLVPVGCGQQLDCEQMLSAEEYEQLLRHI
                     ARKEETAGIELKVTCVPHYQRVIRQEKPQPRRMSKGCLAGSGVLFVSHKGEVFPCGYL
                     PVFCGSILHGTLTDIWHSSPDLARMQDAGQLKGKCGVCCFKTVCGGCRARAFAATGDY
                     MEAEPMCIYQPPRKNSHD"
     gene            complement(42620..43933)
                     /gene="hemA"
                     /locus_tag="PLE88_01375"
     CDS             complement(42620..43933)
                     /gene="hemA"
                     /locus_tag="PLE88_01375"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOM75147.1"
                     /translation="MNSIMQIILVGLNHKTAPVDVRQQLACDAEQASEALGRLKAAWS
                     ESEFFLLSTCNRVECCAVVSRSAGPTAEQLARWLADFRSAPFEQIQPCLYIKTNEEAA
                     AHLFTVTAGLDSMVLGESQITAQVKEAYKLACSCRTTGKILNHLFHEAFRTAKQIISH
                     TSIASRRVSVAGVAVGLAKQLFSDIQSAKIVVIGAGQMGQLLVEHFQHEKCRHVTVVN
                     RSPQRSCRIAETHDCSVVAWEHLEEEIAQAGIVVGAACAAQGYLFTKERIKPLMQQRR
                     NRNLLIIDITVPRSFEPAVGDIENVYLYSIDDLAQAAQDNIKLREGDLELAVGIIYES
                     VSAFMDWFGTHQVGPVIGQIKEAFEAISELEMRKFFAGPRQEAHCRETMEASIRRVVS
                     KLCHCVIKNIDLLSREHGPEEAQRFAQHILDNAQQIISEDIKKNQ"
     gene            complement(43926..44810)
                     /gene="ccsA"
                     /locus_tag="PLE88_01380"
     CDS             complement(43926..44810)
                     /gene="ccsA"
                     /locus_tag="PLE88_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOM75148.1"
                     /translation="MTELMLSEKLLLGGLIGFSAVSSILAYVQTIRDAGRIKEAVGIL
                     LGLQIAMAGMLLGLRAAAIEAFPLTGVFESILAVLVFIGFIILCLWSLMRRVWFSSVM
                     AWTFLGIVLLAWAAARPVSSLNQTVRTPLVIVHSLSMAIAGAMIVLGGAAGLLFLWSR
                     RRLKSKQLLGLLGKMPTIETLEHLMLAALWLGFAALTAGLVSGIGLAAVKSAQLGLTA
                     RDWAADFKVVLIAAAWLGLLAVLVLRHLSAFSGKTAAQAAVFLCLVILFAFVGSRLFY
                     TSRHQFEMAPANPQSLIE"
     gene            complement(45153..46376)
                     /locus_tag="PLE88_01385"
     CDS             complement(45153..46376)
                     /locus_tag="PLE88_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOM75149.1"
                     /translation="MKKSYLLGTLVILLAAMSVSAQDWPQWRGLNRDAKVSDFNPPAQ
                     WPQKLPEKWKVTVGSGDATPALVGDKLYVFTRQDDQEITRCLDAGTGREIWKDQYTAS
                     AVTGAAARHPGPRSSLAVGDGKVVTLGVGGVLSCLDAASGKMVWRKEPFPQSVPRFFT
                     AMSPLIVDGKVVAHLGKEGSGAVIAYALADGEEKWRWADEGPAYASPVLLTADGVKQV
                     ITLTEKSIVGLDLADGKLLWQIPFVPQGRAYNAATPIIDGQTVIYTGAGRGTFAMKVE
                     KKDGGFAPVELWSNSEVAVQYNTPVLKNGLLFGLTNGGNLFCLDAATGKTNWLDTVSR
                     DRSGFGEIVDAGSVLLALPSSGQMLVYKPNGQQYEEIAQVQVSETPIYAYPVIAGNRI
                     FIKDQDNIILYTVEE"
     gene            46812..47249
                     /locus_tag="PLE88_01390"
     CDS             46812..47249
                     /locus_tag="PLE88_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75150.1"
                     /translation="MPSEQFRQEVFGLTEPEGPVLIFVDDRYQAYANDPERAAFLRKN
                     PDILQYLCRRIDDGNDPCVLPMKDGCTAGTQLADERGHCGYFLIFLPGYTRQTAQANM
                     DLIELILAQAQLIFCLIEKNSRFCGCQTSISNLISPEKTANCL"
     gene            47395..48873
                     /locus_tag="PLE88_01395"
     CDS             47395..48873
                     /locus_tag="PLE88_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOM75151.1"
                     /translation="MKFQLFKNGKPIEEYSLAGAYLFGTDMIPLRHVDKIEFKDGEIE
                     CIKKSQDAAGLSLLWPVEDNGMLMLNTTRLPDRAEPYNLNVELARARMMQITLKREDW
                     ALFDPSDELEGMAQEVQDLFIQALQHSGVPERASMFADEALKKGIEFSEKLAAKHAEQ
                     YLAVRFRNKALGRHSLGCELNLNQIADEKYRKYLLDMFGFITIPVNWAEIESEKGEYD
                     FSKMDQCIQYLSGHRLALCAGPLLRFSPDSVPEWLLNEKADFEKIREYAYDFVTKVVS
                     RYSKFIHAWRVISGMNALNCFGFNFEQVIEMTRTACLAAKAVDVKSRKMVELLYPWGE
                     YYAHDKTTVPPLVYADMVIQSGISFDAFGVQIHFGRDIPGMHVRDMMQISSRLDCFAA
                     VPKTVHITGVSIPDAHGPGDRSSERAGSWRKSWDQSLQASWIEEFYKLALGRPFINTV
                     TYSCLADSSGSPMNSCGLLTENLTPKKSFMVLAKFQRTILQR"
     gene            48891..49346
                     /locus_tag="PLE88_01400"
     CDS             48891..49346
                     /locus_tag="PLE88_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOM75152.1"
                     /translation="MRQAPSHSEYLQKIHLWANGCASAAAAGLLAMMLPAICLYTVYR
                     TPPLEARPERINPNTASLASLVRLPGIGKARALDLIAYRSAQPQPAFRSANDLEAIKG
                     IGPKTAEMLAPWLTFEEEQELQMDKPDTTHFRSAVPQKILLYPTLPDIK"
     gene            49514..50014
                     /locus_tag="PLE88_01405"
     CDS             49514..50014
                     /locus_tag="PLE88_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75153.1"
                     /translation="MADWEIKKPLGQCSGTGRPFEIGEEFFAALVETPQGLIRQDFSA
                     AYWNEQKPAVYCFWKSRMPNPEQKKRLFIDDEMLMAFFERLAEETEPEKIQFRFVLAL
                     ILMRKRRLKYEGCIFENGREVWSVRIAGQDRIEKVMNPRLTEDQIEGLTAQMSQILQM
                     DIEGNE"
     gene            complement(50102..51208)
                     /locus_tag="PLE88_01410"
     CDS             complement(50102..51208)
                     /locus_tag="PLE88_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM75154.1"
                     /translation="MKNGIPSLEDVARIAGVSKSTVSRILNNRLGNGFSVRDEVRQRV
                     LDTVRKLNYRPNLFAKSLTMQSTRMIHIFGGNHALSDLGNIYQTVVNNITQVMESVSQ
                     DYDVTVDMSRHSTDVSEMPAWKIDGAVILAKCTSVTMDEIVQMRIPYVVVNGPCPEDG
                     FSVVPDDIGGTKLAMQHLFELGHRRIAYSGPLPPFEAGTDAPEDYRSPIQFRNFGLEV
                     DVLTCHSSLRDRYETYLSQMRQHGLEPITSAAEMPDTAEDYLHTIVYEKGATAVLVYG
                     HMGALNLLQAAHSLHVAVPEQLSIICFCDEYANRVMSPRLTFIDLCSEKMGKTAAELL
                     LEQIQHPEQASPVTIVLDEKLVIRSSTAPPRPVC"
     gene            complement(51453..52298)
                     /locus_tag="PLE88_01415"
     CDS             complement(51453..52298)
                     /locus_tag="PLE88_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75155.1"
                     /translation="MTGGFRKSIKGSQAAAVTLIELLVVIAVIALLLSVILPALKKAK
                     LYAQRIVCQTNLKTLALSNAIYAAKWDNWYVPLIDTTMTARGEPTWNSNAEFRSIVGL
                     EEASAGGRFVMPEEYLCPADQQADEDYWLQQTGAVYQNYVSYGYNFTDWGPTSKSPAV
                     WAGNIPSSNWSCRFRISTIPSPAGKIMFVDAGDWAAYQPGANYRLYWDRDGQNIVRYR
                     SRNMWYPVYFRHSQGANTAYFDGHIDFRKKEILFYYNPPDSLDGDKSMNDMIWFCNPQ
                     NRAIP"
     gene            complement(52389..53693)
                     /locus_tag="PLE88_01420"
     CDS             complement(52389..53693)
                     /locus_tag="PLE88_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75156.1"
                     /translation="MSRKVSLILMVIAAVWTVWADNLIINGDFEQLPGGTGWSVWWGG
                     NSNRYVTDPLNPANHCAGVWWHDDGIFQTIPIGQGKYEFGGKIMTTQGMVNRNGVIRA
                     SFGPSEVDLAIVPGDAVNTWYFKSGIIDNSSIPYATTLTLNLMMANTGTPPSGIVFYD
                     DIYLGPVGISKQAKFPHPYNGEIVAPSTNVLRWTNPLPYNPAESIHSTVYLEKDDGDP
                     NFQSAPIVVGLTTNSVNLSSLGIFLQPSTKYYWRVNSTDPNNGVPQLTQGPIWTFTTL
                     DDDPPVVDAGSNKYLWLSMADGDGDPTKVTFMLDGTLTDDGKSPVSLLWELIYSEQDP
                     ATVVNIATPTQVDSVVTINGTGFYRFRLSADDAYAHAEDIVEVIVYGSACEAAQGDPT
                     DHYHTYPYIGDVNNDCLVNMADFAILAATWLDCLSDKLGCTP"
     gene            54328..54894
                     /locus_tag="PLE88_01425"
     CDS             54328..54894
                     /locus_tag="PLE88_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75157.1"
                     /translation="MKRVMILLSVLATAGLATANLLTNGDFEDPAGSGWSQWWGGNSN
                     KYAPDPIEGDHCAGVWWMDDGIFQTIPIGAAVYTVSGNIMQENLGNGRIGVIKAEIKD
                     GAGNIWWTQEIPITQDDPAGTWLSGSMTIDNSTAGASLMTINLFMWDKNGWGSGAGIV
                     RWDNISVVPEPATLALLGLGGLLLRRRK"
     gene            54994..>55501
                     /locus_tag="PLE88_01430"
     CDS             54994..>55501
                     /locus_tag="PLE88_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75158.1"
                     /translation="MQKFLIVVLGGFICSSCPAAGVNLLTNGNFESGGIGWSQWWGGN
                     SSVNAADPLEGDRCAGVWWHDDGIYQNLVLDAGTYQFGGKLMTTQTGGLKDRRIVIQV
                     QAGGGSPTQQLQIGPGDSENIWHSVSGFITLTAPASVTVTLMLVSDLGSNPSGIGLFD
                     DIYLSEVAQ"
BASE COUNT        13970 a        14614 c        13380 g        13537 t
ORIGIN      
        1 aaggagctgc ggatccgtca tgctgtccga atggcctttt ttttcggggg gtttcaagcc
       61 ttgatgccgc tgctggggta tgcggccggc agtacgctga ccggatttgt aaaccgctgg
      121 gaccactggc tagccttcgg actgctgacg gccatcggca gtaaaatgat ttatgaagcg
      181 gccaaaatag aggccgtgga aaaacgccct caagacccct ccaacatcgc cattttattc
      241 gcattgagca tcgccacaag catcgatgct ctggcggtag gtgttacatt gtctctggtt
      301 acaacccaca tcgcagaagc cgtcttgctt atcggactga ttaccttcgg aatgtcctat
      361 accggctatg aactgggcaa gcgaatcgga catttttttg aaaacaagat tgaaatcctc
      421 ggcggactga ttctgatcat cattggtctt aaaattctgc tgactcatct gtagcgctgc
      481 cccaaaaggg gatgcatccg ggctttcaat gcaggaactc ttgaacagaa tcaatcttgc
      541 ggcgtacaag ggggcagtac catttaaaag acagaactta tggatataca aaaaaaatac
      601 cagacgcttc agaaaatcct gaaagaatac ggaaaagcag ccgtcgcctt ctccggcggc
      661 gtggacagca gctttctgct gaaggcttgt gccgagacat taggccggca caatgtactt
      721 gcctgcatcg gcatcagccc ttccctgtct ggatatcagc ttcagcaggc acgccatctg
      781 gcagaactga tcggcgtacg gctgatcgaa gtcagactgc acgaattgga cgatcccaac
      841 tataccgcta accgcgccga ccgctgtttt cactgcaaga gctgccaatt ccgcatgctc
      901 ggacaggccg ctgccgcaga gggcttccgg cacctgttgt gcggttccaa ttacgacgat
      961 caagatgact accgtccggg caatcgagcc gcacaaatgc tgggcatagc aagtcccctt
     1021 atgcaggcag ccctgaccaa aagcgacatc cgacagctca gccgccagct gggtcttccc
     1081 accgccgaag aacccgcttc gccctgtctg gcatcccgca ttgcctatgg ccagccgatt
     1141 accgaggcca agctggcaca ggttgaacag gccgaggcct ttctgcgttc actgggtttt
     1201 cgagaattcc gggttcggca tcacggcaca gtcgcccgct tggaaattcc ccctcaggaa
     1261 atagagaaag ccgccgggcc ggagcttcgg aaacacatca cagaagaatt aaagaaactg
     1321 ggattctgct atattgcact ggatttagaa ggttttcgca gcggctctct gaacgaaatg
     1381 ctgaccgacg cagaaaaaaa caccgaacag cagccgccta attggtcaaa ccggcaatag
     1441 cagctccaat aagggccgca tgttcaacac cgatgatttc ggtataaatg ccgcgctggt
     1501 tttccagatg ctgcttcaga taaaattcaa cacgggattt aagcgttttc atctggtaga
     1561 atgtcgttcc ctctgcaacg atacaaatcg gacaggccgg attggttccc tgtccgcatt
     1621 tcaccgccat cgccgagagg ttaacggcag ccagcttggc ggctcgctcc accagacggt
     1681 cagcaatcac atacgccagc acggcatctt ggctggtgcc gcccgccagc gcccccgcaa
     1741 gaggattgct cccgcagggg taaagcataa acgcattcat ttgtttggtt gacaggtcgc
     1801 ttaactgccc gatcgccttg gcggcgccgc cggagagcag gccatcccgg caagcccggt
     1861 gaagcaccgt caaaaacagc ggtcccagat aggcccccga aatcattttt tcaaagaaat
     1921 gtacgccggg attggtagtg gatgcatcga actggcggtc gatttgtccc cagtgtgctt
     1981 tggccatgcc gccggattcg gtattgataa tctgactttg ccggggatcg aggcctttct
     2041 gcttggcaat attggcattg ctctcaatat aggcacagtt tgtgcctgta cccagaataa
     2101 agccgatgta gctggaatac agtcggttct ggaagcccac gcccgacagc agcgcagcga
     2161 cggtgtcgtt gagcagaacg atatgttttg acccacccag ccccagccgc ccaatcgcca
     2221 gattcagatt ttcccccacc atctgaccga caacctccgg cgccttgatt tcctttgcaa
     2281 agcgaatcaa acggccgtct cggttagcag ttatctcggt tggatacgaa aagcagaaac
     2341 ctatcttctc agaggcatct aaaacgccgg caaaatatcc ggccatagca tcaaaaaacg
     2401 cgattcggct caatcccgac tctacggcag gcatggaaaa tgtccgcaga ttttcaatga
     2461 ccggcttctt gttttcatcg aaatagaccg ccgcaacccg aacattggtt cctcctgcat
     2521 cggccgcaat cacacgccgg ccgacaggaa tctggttgac cgcctgcaga taagtgggaa
     2581 tcatttctaa actgctcggc aatccggcca aaccggcctc catttccctc aaaaaccgct
     2641 ttatgtgatc atcaaaatct atatcctgat gatccatttc atacgcacag agaaaatcta
     2701 tcgcctgtct gattatcgct tccatcagtg ttcctcggtt ctgcaatgca tattatacct
     2761 ttatttttgc ccgcataata ccacagtatt cgaaacgagt aaatacggga ctgcaaatct
     2821 cttatcatgc cggcggctta taacctaacc cgtcagaggg ttgaagcaac atctgctgcg
     2881 tacattcggc ggatatgccc ggtaaaaaga tttttaaggg attacaaata tatccctcgc
     2941 agcgacctat cctcccgctt gatagaatcg gaaccagttt accataatcg ggcagtcatc
     3001 agataattcc tgcccggctc cattgtgagt tcagggtaaa atatagaatc gtatgcccct
     3061 gatattgccc ccaataaagg ataccacctt cgtacccgta tctgcggttt cgattatcag
     3121 gattcgtagc cgttgggatg gcgggtatga aatttccagg cactttcgac gataatttcc
     3181 agttccggaa aatcggttgt ccagccaagt tccctgcggg ctttcgacgc atcggccgtc
     3241 agcaccggcg gatctcccgg cctgcggtct gcctcgataa ccttaaaatc gcgtcctgaa
     3301 accttacgca ctgtgtcaat aacctgtcgg accgaatagc ctctgccgtt acccaaatta
     3361 taagccaatt ccggctggtc atcgagtctt ttcaaagcaa gcaggtgcgc tttgcagagg
     3421 tcttcaatat ggatgtaatc gcggatgcag gtgccgtctg gggtcgggta gtccgtgccg
     3481 tagattttga tgctatcacg cttgcccata gccgcctgaa taatcagcgg aatcagatgg
     3541 gattccgggc ggtgatcttc gcctaaagcg gcgttaaagc cagcgccgca ggcgttaaaa
     3601 tagcgcagcg ctgcataccg gagccggccg gccgctgcct gaaaatggca catcctctca
     3661 acagcccatt tcgtctcgcc atagggattt atcggctctt tgggcatgtt ttcggtaata
     3721 ggcaccccgg caggcattcc atacacggcc gccgtactgc tgaatacaaa cttctgcacc
     3781 cctgcttttt ccattgcatc aagcagcgtg cgggttttgc tcacgttgtt ttcatagtag
     3841 cgaagcggca tttggacaga ttcgccaacc tcaataaatg ccgcaaaatg catcactgct
     3901 tcaatccggt atttctgaag cgtctccaga ataacattaa agtccccgaa atcccccaaa
     3961 acaaactggg cattctgaac agcgctgcga tgccccttgc tgaggttatc aaacactaca
     4021 ggatgatgcc cttcgcgggc cagcagtgct gtcatgttgc tgccgatata tccggctccg
     4081 ccgcatacca agattctcat tgtgctgcat tccttcgaat cactctttca atgcgtttca
     4141 cgatgcgctg ctcaagctta gcatccgaac ctgcatccag ccattcaatg cgggattcca
     4201 aggccggatc tactgtcaat gtctgtacag aacggctgct ttcggtaatc ccctctgcca
     4261 tcagccccat cccccgaagc ggctgttccg gcacagataa acgcagcacc tgaacccggc
     4321 agccaataca aacctcccgg cctttctggt caaaatccat ttcaacaatc cggcgcaggg
     4381 agtgcaaatt ggcctgcgct gcggctgagg ccgaggcgtt gtcctgccgc cagatttcaa
     4441 aaaattgacc ccctgtcaat ggtcgggtgc gaatataacg ggcatcgtag tcgtattttt
     4501 ctatctcaaa atgcatcttt gtaagcactt tttgcgcggc ctgcattgcc agctcggcag
     4561 aacagtccga cacgcacagc ggcgcagacg atgccccctt ttcgcttccg gcacagccgc
     4621 ctatcaaacc agcagcaagc aacagtaaac tggcaaccag agatgtccgc atatataatt
     4681 atcctaaata taaatccgct atggtgggcg attgtatcga ctgcatcgca atttctcaag
     4741 ccgtttttta ctttttcaac agacttgtca tttcacaaaa ttcttcagat agccgataat
     4801 attaaaagac caaaatgttg ataaaccagt tacaattact ccggaacaaa ctaaagaatc
     4861 ttccgcttgg aggagttatt gaggcattcc tggactattt aagtgtcgag gcaggattgt
     4921 cagacaatac tcttctggcc tacggacgcg atttgatggg ctttgccgaa cactgtcaaa
     4981 ggcagaaaat tgctgatatg gataaaattc tgccggttac agtgtacagc caccttcgcc
     5041 aacttgccca aaacggacaa gccgaaacca gcatcagccg taatctggtg gcaatcaaga
     5101 tgctgctgcg gttcggcgta ctgacgggcc gaatcagcca agacatcagc ggcacacttg
     5161 aaggaccgaa agtatggaag cggctgccgg ggattgccag caaagagcag gtcttccggc
     5221 tgctggatgc cccttgtcca gaggatccct actattggcg ggacaaagcc gttctgcagc
     5281 ttttatatgc gaccggcgca agagcttcag aggtagccgc attgaaaatc caagatgtca
     5341 atctggaaat cgggtatgtc cgctgtttcg gcaaaggccg caaagagcgg attgtcccat
     5401 tcggccggac cgcagcagca accatttcag actatctgaa acaattgcgt cccctgctgg
     5461 caaaaccgca cagtcccgat cgcctgtttc tgtcgcggac aggccggggg ctggaccgca
     5521 tcgagctgtg gcgtatcgtg aaaaaatatg ctgtccgggc cggaatgccc aaaaatctga
     5581 cggttcatac cctgcggcac tgctttgcaa cccatctgct gtccggcgga gtggatttgc
     5641 gtgctttgca ggaaatgctc ggacatgccg atataaaaac cacgcaaatc tatacacacg
     5701 tggatcagga ccgcctgcgg acaatacata aaaaatacca cccaagggct taacgtcggc
     5761 ggcagctttt ctttcggctg ccggaacagt ctattgcagg ggtgtcagaa tgtattgttc
     5821 atccgaatgg gtatcgaaag cgatgaaacc gtctttatag acggtcttaa ccgctgtacc
     5881 attccgcaat gtcactgaat agtttcctcg cagtcggcag ggatggcctc tatcggactt
     5941 gataacagcc cgagtcagcc gaccgtcttt ccattcaata tccgcttcaa aggcattctg
     6001 ggcccgcaga ccggttatcc gtcctgacgg ccaggcatcc ggcagagccg gcagcaggtc
     6061 aataaatccc agatgacttt gcaggagcat ttcggcaata ccggctgcag cgccgaaatt
     6121 gccgtcaatc tgaaacggcg gatgggcatc gaggaggttt tcatagaccc ccccgccgtc
     6181 actcatatca agacgcgtat tcgatgtcag ccggagagca tttttaagca gtgtatgagc
     6241 ccgattgccg tccagcaatc tggcccatgc gcaaatcttc catgcccggc tccagccggt
     6301 tccgccgtct ccgcgggcat tgagcgacac ccttgccgcc tgagcatcgt ccggattcat
     6361 cagcggtgaa atctggctgc cggggtacaa tcctatcaga tgggagatat gccgatgcgt
     6421 atcctgcgga ttgtcatcgg catatttcca ttcccgaagc tgtccccagc tgccgatccg
     6481 cacacctgta tccagtcggg acagtttttc ctgcagcagg gctctgaatt cagcatccgt
     6541 cttgaggatt tcagcggcct tgacagtgtg agtaaataaa tcccaaataa gctgctgggc
     6601 ataagcaacg ccggtctgcc atgggccatg ttcaggcgac cattcatccg gagccgccag
     6661 cgttccgtcg gcatcagccg tcagacggtc cagccagaat tcacaagcgc ttttcatcac
     6721 gggatatgct ttctgcgaga gaaagccgct gtctaatgta taggcatagt gctgccagag
     6781 gtgcatacag taccatgcat tggccggacg gttccagttc cagtcggaat acccaaagat
     6841 attgttctgg gtcttcatcg tccagccgcg gcagcccagc gaagcagcca tgttcgacca
     6901 cgatgtctgt accgctgcct cattataaat ataatccaaa aacggcagat ggcattcgga
     6961 cagattggcc gtttcggccg gccagtaatt catctggaca ttgatgttgg aatggatatc
     7021 acactcccaa ggcggccgat tggaattgtt ccataagccc tgcaggttcg aaggcagggc
     7081 catgccccgg gagcagctta tcatcagata acggccgtac tgaaaataca gcacgtccag
     7141 cgccggatca tacgaaccgg cattatagct ggtcagcagt tcatctgtcg gaatcgccgg
     7201 cttgctgtcg ttcagagaca aacaaacccg gttaaatagg gcctgataat ccgcaagatg
     7261 gcgggatttg agctgcgcat acgattttgc cgaagcctct ttgatgcagt tgacaattcg
     7321 ttcctgcagg ccgctgccca gatactcggc acgggtcggg tcatagtccg ttcccccggc
     7381 cagaagtatt gtcacagcat ccgcaccggc cacagtaatc tgctcggatg aacaatccag
     7441 ccggccgcct tcaggtataa ccttaacaga ggccagatag gacagcactg tcagcttgcc
     7501 tgaaattgaa attgcatcag gagcggtgtt ttttgtgccg gaatgcgcat cctgcagagt
     7561 gagggcaaag ctaattgctc ccggcttgcc ggcagtgatg cgcatgacaa tgagtttatc
     7621 cggatagctg gcaaaatatt cccggctgaa atcggtcgaa tcgactgtat agcagacccg
     7681 ggcaatccct tcttctaaat ccaattcccg ccgatacagt gacacgctgt ccggatggtc
     7741 aaagcggaga tagacatcgc caaagttttg atacgccccg cgcgaagtcc tgctgcctgt
     7801 ccagagggtt ttatcgttga actggatatg ttcctctttg gctccgccga aaatcatcgc
     7861 ccccaaaaga ccgttgccaa tcggcagagc agaagtcatc cagtctgctg ccggctgacg
     7921 ataccacaaa cacagcttgt tcgccggagg accggcaaga ctgtcagccg ctgctgccat
     7981 aggatggatt gaagcaatcc ccataatcag agtactcatc agccatcgca ttcgctcatc
     8041 tcctttgtct caataccgga tgtctatacc cattatatct tacagcagca tcactataca
     8101 ggattgcggg caggtaaaaa agagggcggt ctttttttct gcaattttac gggatgcggt
     8161 ccagagaaga cgccgcagaa gaggataagg cggcatattc cggcagggcc gaaggtttca
     8221 gcagtatgcg ggcgattacg ggaaaatggt ctgacggata gcgtccgttt tctccggcgc
     8281 ggcggtcaat gctgacatcc agagcctgca gtgtttcaca gatagggata tggtcgatgg
     8341 ccggccccga gatactgccc cgaaaactgt gacgcgttcc caagctgggc ttgcccgaat
     8401 gaacagagga ccaggcatca accattcgcg gatacggcgt ttgaataccc tgtttctgca
     8461 gatagcgcat tgcaggattg tccagttcca tattgaagtc gcccataaca atgaagggat
     8521 cccggctttt gcgtgaagcg acttgacggg ccagcagttc gacgcttttc tggcgggaat
     8581 gctgcgacaa attatccaag tggacattat agacataaaa cgcctgtccg ctctgccttt
     8641 cggccagccg gacccagctg cagatgcggg gccatagatt gccccagtcc tttgtccccg
     8701 gcacagaggg tgtatccgaa aaccagaatg tcccggaatc cagcaagcgg aagcgatctg
     8761 tccggtaaaa aatggcacag gtttcacctt gccggcggcc gtcatttctg ccgacagcat
     8821 agcggctgta acagggcagc gccatttgaa tatcacaaac ctgctcatcc tcggcttcct
     8881 gaagcccgat gacatcagcg ttatggtccc gaagcatatt aaagacgata ttcttgcggc
     8941 ggctccatcg attgggacca tctactattg tatcattgcg gatgttgaag gtcatcacct
     9001 tgacaacgcc cggctcaatt ttaggtaatg ggacttgctc ggatggcgtt cgggccggca
     9061 ttgtggggat aaagaaagac aatactgcca atcctgccag agacaacctc ctgatgtcat
     9121 tttgattcac ttagaactcc caataataaa ccaagacagg caatccctga cgctatgggc
     9181 atccatccgc tttgggcgct gcttgccctt cggacatctg ctggagaatg cctaaaagct
     9241 gacaagcctg tactgtttta aatcccctca ctcattaaat acggtaagca aaggaccaaa
     9301 acttgaaaaa ataagaaaat aatttccccc ctgcagccgc agcatctggg ctgtttcaga
     9361 ctggaatcga ccgagttatc gattatttcg atgtgaggtt caggcaagcc gccacgagaa
     9421 agtggtctga aggccagcgg ccattaaacg agcgagcatc aatagccact tcactaattt
     9481 ctatgccggg tccgataaga atatgatcaa tcttggggcc gcgggcagtt ccttcaaagg
     9541 cgtggtaagt accgggaggc gtttggtctg gatgcagata ctgccatgta tctctaatct
     9601 gattcgaaag aggactgccg gcaagcagat aggccattgc cggttcagac aatcctatat
     9661 taaaatcgcc catcacaata aaagggacgc cgtgtggacg gcgggcaatt tgcttggcaa
     9721 gcagttcaac acttttctga cgggatagtt gggactggtg atccaaatgg agattataga
     9781 cataaaatag tttgccggtc tttttttcct taagacaaat ccaagtgcat atgcgaggca
     9841 gggtattgcc ccaatgttta gagccggcaa tccacggagt attagaaaac caaaatgtgc
     9901 ccgaatccaa ccgtgaaaac cggtcccggc gataaaaaat tggacatgat tcaccctgct
     9961 tcctgccgtc atcccggccg acagctacga cgctgtattg aggcagagcg gcgcggattt
    10021 cattaacctg aaaatccagt gcttcctgca gtcctatgat gtcagctgcc tgatccgcca
    10081 aaaggttgaa aaccagctct tttcgattgg cccagctgtt tggcccgtca tcggctgtac
    10141 cgtagcgtat attaaatgtt actgcgttaa taatattggg gtcatctgcc ggaaaaacaa
    10201 ttttccggcc agccgcctta ttttcggtta cacagccggc taatagaatc cctaaaagag
    10261 ctgtcactgc caaaaatcgc ttcataataa gcttccaata aaaatccctg ttcgttattg
    10321 cctgctatga tgattataca cccagaatgt taggaattca aacattgtgt tattttgctg
    10381 cttagccggc aggaagaaac ggctttgatt ggaattggtc ccattgggat tttatggctc
    10441 tgtgttttcg ggcagctttt gcatgcgacg gcagtcaata acaatagtcc agcggctgca
    10501 agccgcaatc cagccattga gccgccatac ggctcaaatc cgccaaatct acacgacagt
    10561 cccggttcag atctgcatcc ggaaaagctg gacaagaagg ctctgttctg aaattgcgca
    10621 aaaccacccg gttggactca taccagacat caaacgtccc ctgctcaaat acatatttgc
    10681 caatgccgcc agcaccggca gcattgacaa actgcccttc cacctgttgg gcatcaataa
    10741 tcacaaattc atccgaggga tcggggataa atccatacag taattccact tcgagcattc
    10801 catccagata ggcccgtcca tattctcccc acccattggt cagcaccagc cccccgtact
    10861 gccagtatgt atccctgccc ccgatgctga ttttcaatac acctgcggcg ttttgcgtat
    10921 agttatagcc aatgtcaagg gccttccagt ctcctattgc acctaaggca acgcggccac
    10981 tttcattctg gacatctccc caaatccatc cccatccctc gaggatgcct cctttgagaa
    11041 cgacaggatt agccctgtca ccatactcaa gggtaatcgt accgccatcc atgcgaatgg
    11101 tccccttcgg ccagacaact attgattggc cttcatagct gtaagcagca gtcagagtac
    11161 tgtaaaaacc attccaaaga tccccctgca aatggatcat cgccgtgccg ccgcggctgt
    11221 ccaaatcgcc gccgacggcc gctgaacctt gacagtcagc ccatgagctg ccgtagagat
    11281 ttagttgccc tgcactgccg ggctgttccg caagaaccat atttccgctg cagaaaagtc
    11341 ttgagtcggt catattgaca acaccccagc cctgcctgcc tataaaaaga ggcacataat
    11401 cccaccaatt ttcaatcgcc agttccgtct ctcggccggt caggctgatg acggctgacg
    11461 aacccggcaa gaccgccatt tcgagcatcc cgaatttggc tctcgcacca ttcttaagaa
    11521 aaacatccgt tgcgccttcc caagacattc caaaccagcc gtccacatac cactcggaat
    11581 ttggatcttc aatttcaata acagtctgat ttcccctaaa acggccgctt tcgccgtggc
    11641 catgcttaag gacagcattg tcatagaccc gcagtctcgc aagaccaccc actcctccgg
    11701 caaacaaacc gtcccagttg ccgtcaatat tagcgatcca ttctgtattt tcgccggcca
    11761 attccaaaac ggtctccaaa tcattccccc atcccagaaa aactgcatgc gaacttactg
    11821 ttccgccgcc agaaacagct aatttcgatg cattcgcatc gccaatggtt acacaccggt
    11881 accggtcatc ccacccttca aaacgccaat tgtgtccccg caaatccaaa gcaagttctg
    11941 aaccatccac catcaaggtt tgatgcgtgt acggctggtc cagccacacc ccataactgc
    12001 cggcaagagt aaagcggaca tcatcctgct ggccgggaat cccgaacggc agccagtttg
    12061 agggatcccc caattgacca cctgcggaag aagtccaaat ataggtgtcc gatagacaat
    12121 agacagacat ccacatagac aaggtaaaaa tagaaatgaa cgcagcattc ctcataatac
    12181 cctcctgttt ctttatgtac gggtaaaata tatctgcccg aaaaaccaaa tagatatttt
    12241 atcacgaagc aaaacagccg tcaatcaaaa tctctattaa caactatagt acgtccattt
    12301 tgtatttctt ttaggcctgt ttggcattat cggtttgttc tattttggcg gcgtgatacc
    12361 caatttctgc agcaggattc caaaccgctc catcggcaat cccatcacat tagtaaatga
    12421 gccgtctatg cgttcaacaa atgcatcgcc ggattcctga atgccgtagg cgccggcttt
    12481 gccctgccaa tctcctttgc ggatgtgttc ggcaatctgg gcttctgtca gcaggcgggg
    12541 gtagacaatc gtcgtatcgg tctctacaat ttcgatggtt ttttctatgc ataccagcgc
    12601 cagagccgtg atgaccttat gcggcttgct gaacagtttg cgcgtaattg cctcagcatg
    12661 tgccgcatca tccggttttc cgataatttc cccgtccaaa tccacaaccg tatcagcgcc
    12721 aaccaccagc gcattggtaa agcgagcggc cacatctttg gccttggcca gagccagctg
    12781 ctgtgcatag atgtctgaag tcaggccttg ggggtcatac agggattcat ccacattgga
    12841 aggaacaacc tcaaagcaat agcctgcttt ctgcagcagc agtttccgtc gcggcgaggc
    12901 cgaagccaga atgaattgag atttcggcag ataggccatt gatagtctct gattagacat
    12961 tgtccgtaat accccgatca acaaaagtaa aatccttcag aacctgccgg aactgcatgt
    13021 tttagtcctt caatcgaact gcataaacaa ttttccggca gaaatcctta tagtctgcat
    13081 ccaacgtcga aacatctccg atataaaaat taaacaaagc cggcgatacc tgcatattcc
    13141 agccgacagt cagcggccgc aagcggtctg ccgccaaggc cgctgcctcc ggcggcagcg
    13201 gccacgtgcc atcgaccgca ccaaagagaa gcgcatggta ccggcgaaga taaataccat
    13261 agttgtattc ccgaaggaat cgaacgagag catagttttg ggcataaaag gccgctacgg
    13321 catcattgct gctgtccaat acctgaccgg gattgagaat tataagtgaa ggcagcgaaa
    13381 taaggctgct ccgctgaaca gtctccttga gcgcccccag ccgcatcagg ttgtattgcg
    13441 gctcgaagac gaaccgcccc tgcgtatagc gacaggtttc aaacaatgcc gcgatacctt
    13501 catccagcca gctgggaagc cgatacgcaa acgtctgctg attaaactga tgccagactt
    13561 catgacccaa aacagaaaac gtctgctttc gtccaatatt ataagcagca atgacccccc
    13621 tgagagtata ggcaccgcgc tgtatcctga ggtaaatcgc ggcatcggag ccggcaaacc
    13681 gatgggtaaa ctgttcccat tggctgcggc tggaaaataa atagacttcc atcggcctgc
    13741 tgatagaaag cggctggggc agctggctct ggcaggccga gcaggccgcc tcgagaaatg
    13801 ccggaatctg ccggagcatc aacggttcca gcagagttgt gtacaagaca taatggtctg
    13861 tgcgcacaac cagcccctgc ccttcctcac tgtgccaaac ctgcacgctg cggatagccg
    13921 gctcatcgtc taaataggca gcaatcgatt gagggctgtc agaaagacag gcagataaat
    13981 gcggcgaagc tacccctgtc tgccggcatc cgctcccaaa taaaagacct gccgcacaca
    14041 gcagtccgca taaacagcgg gccttgacaa ctgttgggct gctattgaat ctcattccag
    14101 ccggcaccga agacatcggc actgaacaaa taggtttcaa cgcggggatc tgttttccat
    14161 gcgtcgggag gcagcccggc cttgtgggca cagcaatagc tcaaaaattc ctctgcgctc
    14221 catccggttt cggttgccac ctgcggcaga aaacagccgc agcggaggcc atctgtaata
    14281 tagatgccgt cgattccaag ccgaagcgat aaggggtcat ccgttttttc cagactcgat
    14341 agaacagaga tttcaatatc cagctcgggc agttccgacg cacggatgcg gtctccggca
    14401 aaccgcggat cgcgcataca ggaagaatac gccatttgag aaaccatctc aatcagaggt
    14461 ctgtcagcgg taaactggcc gatgcagccc cgcagctgat cgcggttttt gatggtaaca
    14521 aaacagccct gcttttcaag cagaaccgga tcctctgtgt ggggcacagg agccggcctg
    14581 tgatggacag cggcatcaat tgtttgtttg gcagtcttta acagtatttt tttctggttc
    14641 tcattcatct gcgcatgtcc ttaatgatgc agaagtttta aaacagcaaa gccgccgatt
    14701 aacagcataa caaaaaccag agacagccag ttaaaatatt tatcgataaa aggccgtatc
    14761 tgttcaccct tccagccgaa aagccctgcc accagaaaaa accgtgcaga acggctcagc
    14821 gctgacgcca gcaaaaaacc ggggaacata atgcgggcaa tgccggcggt tattgtgcat
    14881 actttgtacg gcagcggagt gaaaccgcag gtaaaaacaa tccagaaatc atatttctga
    14941 tagccggctt gaaacttatc gaaattggtc tgcgtaaaac cgataactcc cagatgagca
    15001 aatacccatt ggccgatctg ctcccacaga aacagtccaa tcagataccc cacaatgccc
    15061 cccagcaccg aagcaataga acaggacacg gcaaagcgca gccatttttt gggggccccc
    15121 agtgtcagag gagccagcag cacatccggc ggaacaggga aaaaactgga ctcggcaaaa
    15181 ctcagcaaaa acagggcacg ctcgccatag ggggtatcag caaaatgaat cacccagttg
    15241 tacagccgac ggtgccaatg ccaccaggca ggcgctgctt gaacagtata agacatttaa
    15301 ggtcctcggt aaaaaaaggt tgattgccga cattagaaga tgatatattg cagaagcaag
    15361 aaagtcaaga aatcccgaac agtccaatta tgacctattt aaatcagttt gaaaccgccg
    15421 gcaacggcct gctggtgcga gcgccggcca aaatcaacct ttcgctgttg attaaaggca
    15481 agcgtccaga cggctaccac gagctggaaa cgttgatggc aaaaatcaac tggtatgacc
    15541 agctgctgtt cgagcccgga aaatcggcag gcattgaatt gctctgtcaa ggcccctgct
    15601 gggcaccgga gggagaagat aatttggtct atcgggcctg ccggcgtctt ttggaagaaa
    15661 ccaaaacaga tattccgatt cgcgtaacac tgactaaaaa tattccggca ggaacaggac
    15721 tgggcagcgc cagttccgat gcagcagcgg ctttattagg tctgaatcgc tttggaggcc
    15781 taaaccaaaa aaaggatatt ctggaccgtc tggcagcaga attgggaagc gatgtgaatt
    15841 tctttttagg cggaccgctg gccttctgta ccggaagagg cgaaaaaatt gaagaaattc
    15901 aacccccaac tgacttctgc gcacttttgc tgattccaaa cataactaca tcaacaaaaa
    15961 gggtttacga aaactaccga cactctgaca aagactatct gcggtttagg gggataataa
    16021 atccgctttt agcaaaaaag agtattgatt ctatagccaa aatatgcgca aatatgttag
    16081 aatcgagctg ttttgagctg catccccagc tgcagcagat caaagaggtt tgccaacagc
    16141 agcttggctt gaaggcctgt ttgagcggca gcgggtctgc gatgtatctg ctgatggacg
    16201 aaccttgtgc cgggctggag cgactgcaag accatttgaa aaaggagtac agctgcgaaa
    16261 gcagaattgt caacaacaat agatggtaga tttttcatga ggcagtcaaa atggagatca
    16321 gcgaagtcag agtcaagatt gtggccaatc aggatgatcg tctcaaggcg ttctgctcga
    16381 tgacccttga caatgaattt gtcatccggg acattaagat catcgaaggt acaggcggat
    16441 tattcgttgc aatgccgtcc cgtaaaatga gcgatcattg tcccaaatgc ggcggcaaaa
    16501 attatctgcg ggccaaattc tgcagcaact gcggcaccaa acttcctgaa aaccgcctga
    16561 agcaggatcc ccaaggacgg ataaaactgc atgcggatat cgctcatccc attaacgtgg
    16621 cctgccggaa aaagatacag gatgctgtta tcaaggcatt ccgagaagag ctggaacgtt
    16681 ccaaacagcc gggatacaag ccggtcgagc tggatgagga cgatgattat ccgctcgatg
    16741 accatcattc cgaatctgtt tcctaaaaac gggcaggcat ttgctcaagc gtgcttgaaa
    16801 tgcggcccat taaaaagatg tggtaaatcc ggctctgctg aagaaccgtt tattcctcgt
    16861 ttatcagttc ttcaaggact ttgtccagca ccaaatccat ttttgtatcc cagcagtcgt
    16921 cattctgctc gatatgtctg cgtgcccgct cgattttctc agacctgact tcaggcagag
    16981 aggcaataac cttcagcagc cgccctaacg gatttgactg catcgattcg agaagctctt
    17041 cctgcagggc agctacatcc tgctgcgaca cagcggagtc tatccgatcc tgcagcagcc
    17101 gaatagaatc cctttctttg gggtgttcgt tttgtgtgtg ttttgatgtt ttcattattg
    17161 ttctgacctt tgaagcatgg ccaacaagaa gcagacatcc tgcctgtcct gctgcccagc
    17221 gattgaagcc gaaaccgacc tcaatcacct cttgttgata ttcccacgtg tcccttgtga
    17281 gtcagctgta tttaccgctt ttcaggttca attcggcctt gaggccaacc acaatatgta
    17341 gtatattgtt ttttctcttt tgggctgttt ttcggccttt tacactattg gtatcgtccc
    17401 ttaggctatg ttttcttcaa tgttttttaa atttactgca acttattata ttttaatgac
    17461 ttacgtgttt tttgctgatt ttttaccagt ttaggtctca taaataatgt tgctaatcgc
    17521 ctatactgaa caaatccagt cggtatggtg taaaattgga gaaaacaggg aatacacaag
    17581 atatagggaa aactaatttt tttaacaaga aaccgctcta tttttccttt ggtattatta
    17641 tagggcgttc ctgtccgatt cgtactaaat cctcactcac taataggtca agccaaaacc
    17701 atatggaaag aggggattgt agtcagaatc accgatatta acaggtatct gtgagttatc
    17761 ccggggccag ctgtaagaca gtttgccgct tggcttttgg ttgccaaaaa caacatcagc
    17821 cacaccctgc ccctcggttc cgggcagcca tgcggcaata aaagcatcgg ctttatcaag
    17881 aatgctggta ataatcatcg gccggccgga aatgagaatc acaaccacag ggattccggc
    17941 cttcttcatt ttctcaaccg tttcgatgtc ttggctgtcc aaattgagat tatcccggtc
    18001 gcccttcatt tcagcataag gggtttcacc aattaccgca attccaacat ctgcaccgtc
    18061 tgcaccggtt ccatctttgg agaaagtaac ctgcgtattt gttgaaacgg cctgccggat
    18121 ggcattcaga atcgtcgtgc cgccttcagt gacttgtccg gtgcgtccct gccagtcaat
    18181 tgtccagccg ccgcactgac tgccaaggtc atcggcgttt ttgccggcca catgaatccg
    18241 agcgattttc tttgatagag gcagtatctt gttgtcattt ttaagcagca ccagagactt
    18301 ggctgcggcc ttgcgggcaa cctgccgatg gtcttccgag ccgagtctgg ccatcagcgt
    18361 cctgtcggtc agaacagagc ggttcttgtc catcagcccc atcgcaaatt tgacccgcag
    18421 aatgcgcagc actgcgtcgt ctatacgttc catcggcacc ttgccttcgt tgaccagttc
    18481 tttaaggcag tggataaatt cgggatatcg ctgcggaacc atcgccatat ccattccggc
    18541 attgacggaa atcataatgg cagtcttata gtcaggatca atctggtcaa tagcattgta
    18601 gtcggaaata agaaatccct caaagccgag ttctttcttc agcacgtcgg taagcaggta
    18661 tttattggca gagcatttaa cgccgttcca gctgctgtag gaaggcataa tcgtacccac
    18721 gccggccttg acagcgggaa tatacggagc caaatgaatc ctgcggaagg ttgtttcatc
    18781 aacacgggta tcaccctgat cgagcccgcg tccatctttg gttgagccaa aggccgttcc
    18841 cccgtcgccg gcatagtgct tggcgcaggc cgctacagca agcggatccc ccagccggcc
    18901 tgcctgcaat ccccggattg ccgcttggcc aagaagcgat accagctgag ggtcttcaga
    18961 aaaaccctcg tacgttcggc cccagcgttc atcctgcggc acagtaacac aaggcgcaaa
    19021 ggcccagttg actcctgtcg cacgcatttc caccgcggtg atgcgtgcga tttcttctac
    19081 aagctgcgga tcccgtgtgc agcccaatcc gatattgtga gggaagatta ccgcgccgat
    19141 tacattatta tgtccatgaa cggcatcgac tccatacaaa agcggaatac ccagacgcgt
    19201 tttcaaggcg tgctgctgat agcggtcata catatctgcc cacgctttgg ggctgttgcc
    19261 ctcggccgga tcggatgaac cgccgtttaa taccgagccg agaaagtatt tttcaacatc
    19321 cgccggatct ttgataaact catggtcggc ctgcgtcatt tgtccgatct tttcatcgag
    19381 ggtcattttg gccagcagct ggcgggccgg ctggtcccaa agcgacagag attggtctgc
    19441 cgatatttca gaggcagtcc ggcaagatag ggaaagaaga cctgctgcaa tcaaaaacac
    19501 ccgcagtaca acgcgcaaag acatatcctt tttcattttc aagactcctc taatctggat
    19561 tattcggctt attattgtat gccagaccgg ccttttatgt aagccatttt ttctgcgtca
    19621 acaatacacg ggcttccacc cgctcaaacg cctgacagcc aatccgcttt atttcatcaa
    19681 ggcgataatc tcatccaagt catttttgac ctgtaccggc ggattgctga aaatccgtgc
    19741 catcttgctg tggtaattca ctgttgcatt gggatctttg agaagatggc cggtcaatac
    19801 acaggccaca cgggcgttct tgtccaccgt gccgtcggct aacagatatt tcagcccggc
    19861 gatagtggcc gcagaggccg gctcacagcc caaaccgaat ttcccaacct gtgccttggc
    19921 gtcaaggatt tcttcatctg ttacatccag cacaacgccg ttgcagacct ccagcgcacg
    19981 gaggcatttt ttcagattaa ccggccggga aatttcgatg gcggatgcaa tcgtattcgg
    20041 acgatagccg gcggcatcca gaccggcaaa ataggcatcg gctattgcct gattcacatg
    20101 ccctccattc catcgcaatc ccttatcatt gaccagttcg gcaagcgtat tggcaccgcg
    20161 ggcattgatt acagccagac gggggatgcg gtcaatcaat ccgacctctt tcaattcggc
    20221 aaaggccttt ccgaaagcgc tcgaattgcc cagatttccc ccggggacaa taatccagtc
    20281 gggcacttcc cagttgagcc cttcgatgat gcgcagcata atggttttct ggccttccag
    20341 acggaaggga ttgagggaat tgaccaaata gatatgcaat tgcgcacaga catcctgtac
    20401 ctgacgcata cagtcgtcaa aatcgccggc aacctgtatt gtatgggcgc cgtaatccat
    20461 cgactggctg agcttgccga atgcaatctt gccttcgccg ataaacacgg tcgctttgag
    20521 gccgctgctg tgtgcataca gggccatcga agcggaagta ttgcccgtag aggcacaggc
    20581 gctggcgacc gcaccaacca ttttggcatg gctgaaggcg gccgccatgc cgttatcctt
    20641 gaaagagccg gagggattca gaccttcgta ctgcagaaag agcgttcctg aacccattcc
    20701 cagctggcct gccaaagcat cattctgctg cagcagcgtc tgcccctcgc cgacggtaac
    20761 cttgtacggg tcgtcacaga aatccagcag ctcgcggaaa cgccacacgc cggaaaagtc
    20821 taaccggttt tgccggctgg cccaccgctt tgaaaaatcc ctgagcctgg caggcagaga
    20881 aatgcgattc caatcgtatt tgacatccag caggtcgccg catcgggggc atttgaaaag
    20941 cacctgtgaa cagtcaaatt cggcactgca ctggaggttt atacatttct gatacgcacg
    21001 gtctttgttt ttcatacaaa atccctacga agcattttct tctgattata cagacccatc
    21061 gcggccgcta cccgacgatg cctgcatcaa tgaaccgcat atgtcggctg ttcctgtcgg
    21121 cttattccgt tgtcgggacg aatcatcttt ttaatcaatc ccggcagttc agcaatcgtt
    21181 tcgatgcagg caatatccgc cggaacgggc ttattccgat tcgtataggc ctttttaagt
    21241 atcggcagca ttccggcagc caatgctccc ttcacatcgt tatccatccg gtcgccgaca
    21301 taaaccgttc ggtcagcagg aatacccatt cgctcggctg cgactgaaaa aatgcggaca
    21361 tcgggcttgc gaaacgggaa ttcataggaa tacagccgca ccggcagcag gtccagcaat
    21421 ccttcctgct gcagatgccg ctccagactg cttttgtgca cgaaggtatt ggacagcaat
    21481 ccgagtttga gccccatatg ctgcagacag cggagggttg gggcggtttc tttttctacc
    21541 gccccttttc tggacaggcc tacataccac tgccagttca gttcctgcca ctgctgttca
    21601 gacagggtaa atccgcgttt tcggccgtag gccttcagca attccagcga attaaaatca
    21661 tcacccgtca gccatgattt gagcaaatgc cagcggatgc cccagatatg gacgcgccgg
    21721 taagccctca gcgaaccgac cggctgggca agctctatca aatagcggta agagcggttg
    21781 acggcctctt caaagagagc cgctctatcc aatctgccga aattcagcag cgtttcgccc
    21841 aaatcaaaca gaaccgcttg gatctgccgg gagaccatgg actacctcaa tggaggaagt
    21901 ttgtcgccga atatttcctc tgtccaggca atcatctctt catcagaaat ggccgacatc
    21961 cggtcggcgg catattcagc gtcgatgcgg tctttaatgg caataatccg cggatcctgc
    22021 ttgggaattt cgatcgcata acgttcttca atccaatgct tgatgcccgc tgcgccgctc
    22081 ttgtcggtaa tcgccacgcc taccggccgc ttcaggaggg cctgcgtgtc aaagcaattg
    22141 tagatttcct cattcttcaa aagaccatcg gcgtgaatgc cggcacgcgt tacattaaaa
    22201 tcccgcccaa ccagcggata attgtgcggc agattgaagc ccagttcctt attggcatag
    22261 tcggccaatt ccgtaatcgc ctgataattg actttggggt tggttccgaa cagctgggca
    22321 tgctctacca ccagcgcctc cagcggattg ccggttcgtt cgcccacccc gaaaatggca
    22381 cagttggccg cacagcatcc gtacagccac gctgtggcag cattgatttc aaccttgtga
    22441 aaatcgttat gaccatgcca ttcgagccat tcggccggaa caccgattcg gcgaagcccg
    22501 gcaatcagtt tcggcagaga gcggggaagc gtgctgttgg gatacggcaa ggcaaagccc
    22561 agcgtatcgc acaggcgcat cttgaccggc acattgtact gccgagacaa ttccagcagc
    22621 ttatcggcaa acggcagaac aaaaccgtca taatccgccc gcgtgatatc ttcaaaatga
    22681 caccggggaa gaatcccgtt atccaaagcc gcctttacta tccccagata ggcctccatg
    22741 gcctgcgaac gggttttttt cagcttcagg aaaatatgat aatcgctggc actggtcagg
    22801 atgccggttt ctttaagccc cagctgcgtt acatacttaa aatccgctgc caccgcccga
    22861 atccagccgg ttatctcagg gaagtcatat ccgcgctgac ggcataactc gaccgcctta
    22921 cggtctttct ccgaatatag gaaaaattca cactggcgaa tcaggtctgt gccgccatta
    22981 atctgatgaa gataatcata aatgcggaga acctgatcgg gcgaataggg aggacgtgcc
    23041 tgctggccgt cacgaaatgt ggtatcggta atccatactt tgccgggaat ctgcatcgca
    23101 accgtctcgt tttcaaagaa aactttagga aattccgtgt aaggaaaaac atcccggtat
    23161 agattggggc gatccacatc ttgcagctgg attttttgcg tcatttaagt atctccatag
    23221 taaggcgtta tagcaatcat atacacgtcc cagtattcgc tttctaccgg tcttgaccga
    23281 ttaaaatccg gcagtagccg aggtgggagt cgaacccaca cgcccgttac gggcagggga
    23341 ttttaaatcc ccagcgtctg ccattccgcc actcggccga tatacgcctg caaagaacga
    23401 tttgttcaga taatcccgct tcaggtaccc cagtattata gaaacgctca acaggttttc
    23461 aagaaaaaca ttggataggc cattttttgt gtttttgagg ttaaaaaagc aaaactgggg
    23521 ggctgctgat ccaattctat ctcaaaatca gacagcaatg ccggaatccg agaggagaga
    23581 gagagtctag tccataagtc agtgattggc gattttctgg ggttattgga cacagcaaaa
    23641 ccagttcatt acagttaatc tttccatcaa agcagccgct aaaatcttat cagagcaggt
    23701 acggccaact ctgccctttg ctaccgcgtg cccatcctgc taggattggt gtttttcgga
    23761 tattccccgc aagacgataa ccggaatctt ttccctaacg aaccgacatc cggtgtcatt
    23821 ttttcagcat accgacatac aatatcatcc tgttccgaag gcggtgattt gcctgctgag
    23881 gccgttaaat accatctaca acattcgaca atatacacat ttttagcgta attttatatg
    23941 ttgtttatag ttattactta ctctctctta cttccttata ctctgtatgt cttccagagc
    24001 tcccatcttc tccggcagca gcaacctgcc gccaaagccg catggcttgc tctgaaaatt
    24061 cagcatccgt ttttcttgga aaagcatagc cctaaaaagc ccgtatgcca ggcctcgtcg
    24121 gtgattttgc tggataaaac aggctcaaaa cgcttaaaaa ctgaggctgt ccagcgtcaa
    24181 ctataatctc cctcagcgac aattatttct tcccttcaat tatcatatct cctgacttta
    24241 aggaaattca gatagtttat tctctttatc taatgtccgg aaattttatt tgccgctaca
    24301 ggtaagaata attgtcgctg atcaattttt aatgctaaag gaagtgattt caaataggaa
    24361 atcatgtcaa tacccctttt tgggaacact catagttgga gagtttagca catgttgggg
    24421 gttttgtccc agcgtcaaat gggacaaaac actatacccc cccaattcga acctgtcacc
    24481 caccacggca tctccatgtc ttgttcacca acttccgttc acccggtgaa tggaggttaa
    24541 tccgccctca cactatgaat ttgggtgagt tggtgatatg aaggtccccc ttagtctgaa
    24601 aacagtgctg tccctgttct ccagctcctt ctaattctct tcagaatggc tccagattaa
    24661 aaactattat agactataaa cggcggagaa aattgtccca cctggcggaa caacaatagc
    24721 acgtggccgg gattgaccac gtgcttcata ttcatccaat ccttgtttgg aattactcaa
    24781 actggatcca gtcaatttca accctgtcgt cgcccttgag aacgacctta atatcctgaa
    24841 gaccggatgg agcagcgctg acattcgatg aagctatcgt ccaatccgta cattttgcaa
    24901 tgactacagg caccttgatc gcagcgtcat ctttgccaat ttgaacttca atcgagccgc
    24961 ccgttgctga cattgctctg actttcaggg ttttcaccgt cttgcctgca aaatccactc
    25021 tgtcgtatcg aatccaagcg tccggcgcag acagaaccgt cttccatcca tcaaatgttt
    25081 tctcaggatt aaggtactcc actgcagcgc cgttctcgct tttggcgctg taacggtcaa
    25141 tctgaatcat gttggttgcc ggggcgtttc cgattccgcg taccgtggga atgactttct
    25201 gaatggtacc gtcctcatta aagtacaaat aatcggcttt gatggctcgg tttttatcaa
    25261 aattcggcga taaatccatg tcatgataga acaagtacca ctgccctttg tattcaagaa
    25321 tcgagtggtg attggtccag cagccgctgg cactttcatc cataatcaca cccttccatt
    25381 catacggtcc catcggactg ctggccattg cgtattcgat ccgttccgta ttattctgca
    25441 catggggata ggtcaggtag taaatcccat ttcgcacaaa ggggaacgga ccttcacgga
    25501 gacctttctt gggaagattt gcaatctgct gcggaggtcc ctcgatttcg accatattct
    25561 ccttcagctt ggcgaccatc aaatccgagt agaacaggta ggcgattcca tccttatcaa
    25621 taatgacccc cgggtcaatt ccgcggatgc cctgaatgaa ggtcggttcc ggcttgaacg
    25681 gaccgtaagg attgtctgca atggcaaccc caatcctgcc tcccgtggga aaataaaaat
    25741 aatatttacc gtctcgaaaa acacagtccg gcgcccacat actgtaggac atgcgggtca
    25801 gccaaggcac ctcgctctgg gttaaaatga cgccgtggtc ggtccagtct atcaggttct
    25861 cagaagaaaa aacatgatag tcttccatgc agaaccagtc ttgccgtgcg ccgctgcccg
    25921 gaggaaccgg aatatcatga gacgggtaca catagatctt gccttcgaac actcgtgccg
    25981 tcggatcagc ggtaaactga tcgaggatca gggggttatc cgctattcca atctgaatga
    26041 gaaacaaaac gaatccaaca ggaataagtg aaatacgacc gaattgattc attgtcaatc
    26101 ttcctacata aaaattctgt tgttagtgca ttatactcag ttcgacgtta ttattcaaga
    26161 gccatcttaa cgttccagtg ttttgtaacg gccttctcca gaaagacgac ccacggttta
    26221 gcgatagcct tggtattagg caaaaccgtt tgatattctt cctcattcaa aaaattatga
    26281 tcaggaggat cttctctcat tttttttata cctctgcagg gattaggtat tttatagttt
    26341 tcctgcatat accggcacag gttcttaacg gagaacaaaa cattattcag tgttctgttc
    26401 ttgagtcccc tgctcatttc actattaata tggcgattga tatgggcatt cgttaaatca
    26461 gtgatgtagt tcacactctc agggagacac tctataaaat gctcttctga gaaaatatat
    26521 aaatcccttg tgcagggagt ataccgttgg caatactcct tccattcctc caccacatca
    26581 gataaaaaaa caggggcaac aaaaacagtc cgtttgagtc gtttttcatt ctcgtcacaa
    26641 tgttttttaa attcaagagc tgttttctta tcttttccaa aggatttgct cccgcggtct
    26701 atttctcctg taggcagcgt acatcgccat gcgactctcc atcgttttgc attcccatca
    26761 tagtacaacg aagccataat tcctatatcc agaccattaa tatcgcttac tgatcaggat
    26821 attaacgccg gcacgttcac ttttcaagct ggtatgaaaa aactggtgca gaaatcaaag
    26881 tgaggtgaaa atttacgcca taatttacgc cattgtgata atagaggaca agaaagcact
    26941 gaaacaggaa acatataagc attgtagtac aaagcagtta aattaaatgc gggcgaaagg
    27001 agtcgaacct tcacaccacg agggtacaag aacctaaatc ttgcgcgtct gccaattccg
    27061 ccacgcccgc ctttttaact aaatctatca acaaacttcc cccgtttttc tttattgcct
    27121 gacaagatta gcccccgaca gagtttcaca cataatcttg actttcccga cagttctatc
    27181 cgctttttta tacccaacct aattccagaa tcttcagttc cgcacagcag agcatcagct
    27241 gcctacgcaa atccccaaaa cagcatcaat ccgcaggaca accatgcttc cattgaagcg
    27301 gcttataaat ttgccgatcc gattaaaata agtcctcctc tgcggacatc tcggaatcct
    27361 cattactgct ttcttcctct aagtcggctg caatttggat atcgctcttg tctttggaga
    27421 tattttcgat gcgtttttcc gcatccagaa gaatcttccg gcaatgctta atcagagcca
    27481 ttcctttctc atactgctgc agactttcgg ccagaggaat ctgaccgtct tcaatcttgt
    27541 ccacaatctc tgtcagagca tcgatggcct gctcgaatga caatttatcg atatcgcctt
    27601 tttcctgctt ttttgccata atcgtttatt ccagcggctt gtttttcgtc tcttccaccc
    27661 ggctgatgat ccggcctgcc gccagctctg tggtcagcgg gtcgttgatt agaacttgct
    27721 tgatatccgt cactaagcat cccgtgcgct gattgatcgt tatgctgtag ccgcgtttga
    27781 gcaccgagcg aggattcagc gcttgtaatc tattctccag agcggctaat tgcaattggt
    27841 ttttattgag aatttggagc atcgccgtcc ggctgtgccc ttccaaagtt tggattgcca
    27901 gctggttttt tgccagaagc cggataggtt ctatgcgctg caccatcgcc cgcatccgtt
    27961 ccagctggtc tttccgatat gccagatgcc gctggactga atgcatcagg cgattggcca
    28021 gctcatctat ccgctgggca gctatctgca ccggacctaa aggcaaacgg aatacccggc
    28081 tggcaagtac agcagccaag agattgcggc tgttctgtac ctgtgagcga atagtatttt
    28141 gcaaccgacg tgccagcgat tggattttct ggagttcctc tcgccagtcc gggacagctt
    28201 ccacacctgc tcgggttggc gtagaaaccc gcttatcggc aaccaaatcg gcaatggtaa
    28261 catccacttc atggccgaca gcacttataa ccggcagttt tgatgcataa atagcccggg
    28321 ccactgcttc ttcgttgaaa gcccacaggt cttccataga gccgccgccc ctgccgacaa
    28381 tcagcagctc caatcccagc tgcttccgca ggcgattggc cttggcaatg gccgccgcaa
    28441 tacacggggc cgccccttcc ccctgaactg gcgtgtcaaa cacatacaac cgagcgcagg
    28501 gccagcgggt ctgaatactg tcggcaatat catgcagggc tgcaccggaa aagcttgtca
    28561 caatcccgat gttcattgga tagcgcggca gcggcttttt gcgggctgga tcaaagagtc
    28621 cttcggcttc caagcggcgg cgcatctgct caaacgcaag ctgcagggca cccataccgg
    28681 ccggctccag tttgtcgcag taaaattggt acttgccgcc gggcagatag acatcgatat
    28741 gtccggcggc tagtaccgca aggccgtttt cgcagcggaa tttgacttgg cggaaatggc
    28801 tggcccacat ggtgcaggga atttgcccgc cggcatcctt gagcagaaaa tagcagtgtc
    28861 cgctggtgtg ctgcttaaag tcgctgattt cgcccctcaa catcagccgc ggcggcaggg
    28921 cttcctcaag cgccgccttt atcattgtgt tcagctgcgt tacagtaaaa atcggaagtt
    28981 gtggttttat gttggccatt atcggggatt atagccggaa acgccaattt ggcaagctgc
    29041 accacagaag aatatccccc gccagcgggg atttgtttat tttcatgcat ttttgcggga
    29101 aaacctccta ttctccttct ggtctatcct gcagtaactt gccaaagtcc tctggaattt
    29161 aatgaccaaa atgtcatcca gcctgtttgc ctcagcatta cagcagggat tttgccgagt
    29221 cgataaagga acaaatcctt gaaagctggc ggggaaattt atccgcagcc tactgtcagc
    29281 aatcagccgc ggcgatgaag attaaaagcc gttggtatag tcaggtctgc cgtcgtcgct
    29341 gtcataggtc aaggtatcag gggcggcctg atcgtgcaga acaatccgct caatgcgttc
    29401 ggcttttttt gtatggctgt ccacagtaac aacaatgccg ctgatgcgga catcgccggc
    29461 agcaacatca aaaggatagg gcatctgcgt gcgaaatgcc cgtatgacgc tttcgggatt
    29521 acggccaagc actgaatcgg ccgcgccggt cattccaata tcggaaatat aggccgttcc
    29581 ccccgtcaga atgcgttcgt cggctgtcgg cacatgggta tgtgtgccga aaacacagct
    29641 tacttttccg ttgagataat agcccaatgc aatcttttcg ctggtggctt cggcgtgtat
    29701 ttcgacaaag agaatgtcgg cttcactttt aagacgaggc aaaagcgtgt caatcttggc
    29761 gaaggggcag tcggccggct tcatataaat gcggccaatc agagaaacaa cagccacgga
    29821 cggccccttg acagtctgat aaacagcgta ttcccggcct acagccgaag gtgaaaggtt
    29881 ggccggacga acgatgttgt tctgcgtttc gagggttggg ataatatcct tcttacgaaa
    29941 gcagtggtcg cccagcgtaa taagatgcac gccgtatttg aggagcttgt cgtaaatctg
    30001 gggggtaagg cccgagccgc cggcggcgtt ttctgcattg gcgataacgc agtcaatgct
    30061 gttatcccgc acaaagctga acagctgctg ggcaagaatc cgccggccgg ggcggcctac
    30121 aatatccccg atgcaaagga cattaatttt catcagcact cccaatcctg actgccgcag
    30181 gacagaccgc cacttttgta cagttatctg gcgtattcga cacagcggac ttcgcgcagc
    30241 agcgttactt taatctcgcc ggggtaggtc atttcatctt caatcttttt agcaatgtcg
    30301 cgggcggttt tcatagccac atcatcgctg actttttcgg catctacaat gacccgaatc
    30361 tcacggcctg cctgaatggc atagcaattt tccacgcctt cgaaaccggc ggcgatttct
    30421 tcgagttttt ccagccgctt gatatagcgt tcaagcgttt cgcgccgagc gccggggcgg
    30481 gaggcactga tggcatcagc cgcagcaacc agtggggtat agggattgtc ggctggaatg
    30541 tcgccatgat gggcttcgac agcattgaga atgacattgg attccttgaa gcggcgcagg
    30601 aaattagcac cgatagcggg gtgtccgccc tcgatttccc ggtccattgc cttgccgatg
    30661 tcatgcagaa gacccgcccg ctttgcaagg gaaccatcca gccccagctc atcagccata
    30721 acctgtgcca gaaatgccac ttcgacactg tgacgcagga cattctgacc gtagctggtt
    30781 cggtaatgga gggcaccgag catcccgata atcttgttat taagaccgcg aacatctact
    30841 tcttctgcgg cttctttgcc cagctggata acacgggaat gaacatcttt tttagcctga
    30901 ctgacaattt cctcgatgcg gcttgggtgg atgcgtccat cctgaatcag ccgttccatg
    30961 ctcaggcggg caacctctct gcgaatcgga ttgaagccgc tgaccacaat gacgcccggc
    31021 gtgtcatcca caatcacatc gacaccggtt gccttctcaa acgcacggat attgcgtcct
    31081 tctcggccga tgacgcggcc tttcatgtca tcgctgggaa tatccaccat tgaaacgctg
    31141 acttcgcagg tctgctcagc ggcatagcgc tgaatcgcca gattgatgat ttctttggcc
    31201 ttttcttccg ccatttcctc tgcttgggcg gttttccgct gaataagctg gttcatctcc
    31261 cgctcgcact catcttccag ccggcttagc agcatctctt ttgcctgttc gacatccagc
    31321 gaagtaatct tcaagagcat attcttctgc tgggcaacga gattggccaa ttccttattg
    31381 cggttattat aggtattgat ttgccgctct aattcgcgat ctttgttggc cagctgcttt
    31441 tcctgctgct ggatcttctc ctgctggcgc tcaaggagat cctgcttttt tgtcagttcc
    31501 aattcctgca tacgcagctg attttcccgg gagctgactt cggcagaaaa ttcctggcgt
    31561 tttttcataa tctcgccggc ggcatccaga cgagcttctt tcaggattgt ttcggcctgc
    31621 tttttcgccg tttcgatgcg tgcttcaatc tcctcctgag tgaccttggc gcgggtcttt
    31681 ataatcccct gtaatacaac aaaaacgatt cccccgccag ccagaatacc gatgaccacc
    31741 cctataaaaa tacttgtcgc aacactcatc tgagccagta tttccattgt tgacaccttt
    31801 cttgaatgca gttacttgtt cagacctgca gaaaggctgg aaatgtcagc agactgctga
    31861 agagacaata gaggatggtg taaattcagg gtatcagtga gaacgaacca aattccgcat
    31921 atttctgcct ccatgggctg agcaaacaga ctctatttga agcagaagaa gttttctcga
    31981 agcggaaaat atgacatcct tttgtaatat cccccggcaa ttccattttt tgccatagga
    32041 tttgactgaa tttatcacca aaattccctt tcgccaaact caccgacttg tcaggattgt
    32101 ggacttggcg aaactaaaat ttttaattcg cctttcgccc ttccggacaa tatcggtgct
    32161 ctcaaagcac tgcttacaac cagcctttac aggtctggcc ctttagcccc gcctgttaaa
    32221 gaacagaaca ggtatgaata aggagctatc tactattatc aacaccgaat atactgcatc
    32281 atacattatt tatattatcg gtcaagcaaa aacattcctt tcactaatct tgccggcaaa
    32341 aaaagtaaaa aacatcatat tttgtatccg atgcataatg tccaataatt attttatcgg
    32401 acataactga ataagaatat gcattatttt tcgaaaaagt cagacttgca ccgcaagctg
    32461 attattttga atccctttca agccgaaaaa agattgccga gcaaggagtc ggttgtgaga
    32521 gattagggat tatccaacga atacctgtcc ttatggacat tttggaattt ttatgcgaag
    32581 aaaagatgtt ttaactaccg gtcaggtggc gcagttgtgc aaggttgctc cgcgcacggt
    32641 gacaaaatgg tttgattcag gccagcttcg cggctatcgg atacccggtt caaaagatag
    32701 gcgcatcccg cttagtgaac tgattcgatt tatgaaggaa cacgatattc cgacaactga
    32761 actcgaaaaa ggaaaaattc gtattcttat aatcgacagc cgaacagatt attcccaaaa
    32821 acttgccgaa gaactgaaaa tgcggggtag ttttgaggtc cagtgtgcct acaacagttt
    32881 tgatgcaggg ctgatgtccc tgaaattctc tcccaatatt atcctgattg atttaatgaa
    32941 cagttccatt gatgcgcgcg ggctgtgcaa tgatgttcgc agccgggagg aattgtccga
    33001 ctgcatgctg attgctttgg ccggcggact tggggaaaaa gaagctcaga acttgcacaa
    33061 acagggattt gacgctgtcg taacatccac aaataatatt aatgccattc tggcctgcat
    33121 tcagaaaaac tttactattc tttattgagt ctgatgccgt ttgacaaaca ttccgccggg
    33181 aagctgccgg atgagtccct tcaactgcaa actaacagcg gccgcatgaa cttgaccggc
    33241 tgtcagtttt gtttgtgcaa tcaaatcttc cacatgaaca ggttctgaat taaggcaatc
    33301 gagaatagcg ttttcttctt tggtcagatt cagacgggat acatcaaaca aacttttttg
    33361 ggcgttctgt tccgcttgac cggccgctga tgcagcatag tcttgtaagc cgtcacccac
    33421 attgcccagc gccgccataa tatcgtcgat gctgtcgaca aggcgtgcgc cttgtttgat
    33481 gagttgatga cagccggcac tggcaggcga atcaatccgg ccgggaacag ccattacttc
    33541 acggttattg tccaaagccg cctgtgctga gatgagagcg ccgcttcgat gggctgcctc
    33601 aaccaccagc acccccaatg acaggccggc aataatgcga ttgcgtccgg ggaagtttgc
    33661 tgccagcggc tcatacgtca gcggaagctc actgataaca gcgccggatt ccgccgcctg
    33721 ctcaaaaagc ttttggtttt cagctggaaa cggattggcc agcccgctgc cctgaacaac
    33781 gatggtgcgt cctttggcgg acaaagcccc gcgatgagcg gcagcatcga tgccgcgtgc
    33841 gccgccgctg acaattgtaa agccggcaga agccagcaga tgcgcaaaac ggccggcctg
    33901 ttcggttccg taaagcgtgc agcggcggct tcctacgatg gccaaggcca gcgtgtcggc
    33961 tcgcgtcaat gttcccttga catacaacac cggcggcgga tcgtaaatgg ccttcaaagc
    34021 aggcgggtag cgcggatcat gcaggtgaac aatccacact cccagtttat cagcatgttc
    34081 gatttctttt tcagcattaa aagtattccg cgttcgggca atctgctggg ctgtggtttg
    34141 actgattcct ttcacctcgg ccaaccgggc agccgaagct gcaagaatgc gatccacatc
    34201 tccgaacgca tccagcaggc gtttaaatag tacagggccg ataccgtctg aacgaatcag
    34261 cttgagccat ttttcaatat ccgcagagtg atgctgaacc ataactgccc ttctataaat
    34321 tgggataaaa tgtatcacca caatgcggca gcgtcaatct ttctttgatt gccgggcagt
    34381 tcaaatcagc cgtttaacgc ggctgcagct gcactgctgg ctctctggca gatccagaag
    34441 atcttcaaga ttcctttgcg tgtctcggct gcgttatccg aaatgtttgt tagaaacgga
    34501 cctgataacg gactccaacg acagtaggtt cttcgaccca gcttgccttg tcggggtagg
    34561 catatttgaa gccggaggag taggaataac gcttgttgtc gccgataacc ggtatgttct
    34621 ggacaaaaaa tgtcaggtct gccgatttat tgatatgata ggtcagcgac agattcgctg
    34681 tgatctcgaa atcataggcg tcttgctctt taatatgccg tatttgggct gcattgccgc
    34741 cggcttcgct gagggcatcc agaccgattc tcgagccctc aaagccccac agcgtccgca
    34801 tatcggcatg gaaggtcatc ttgccgccgt acagaccgat attggtataa agcttggtgg
    34861 cattcgtcgg ccagttgttc aaatcattgc ctttggaggt aattgttata ctatcgctta
    34921 ctttcagata ataatccgaa tagctgatgc ctgaaacggc tatattttct cccagttccc
    34981 aatcaagctg cttgagaaac gagtgattaa gaccaatctg tacattatcc ttgctgtact
    35041 ggccctccaa ttccagaccg gctgtcttga gctggccaac aggagcactt cgccggagtg
    35101 cccagtccca agcaatgact tcgtttttgt tgtaaaaacc agatgcctgg aatgtcagat
    35161 ggtctgtcgg tttggcggta taaatcagct caaaggtgtc cagcttttca ggtttattgt
    35221 cctcatcgag tttatgattc atatacaatt cttcctgtgt gttcatccgt accgaccgct
    35281 gggcgataaa tttcaggaat tgctcggaat ccagctcata aatccatgca aaccgcgggg
    35341 aaaatagata atcggtataa gaatgcttat cgagacgggc tgaccacaga gttgtcattt
    35401 tgggactgtg ctttatattc acttcgccca gaaatgcatg actggcagtt tcccagccgt
    35461 gcccgactgc aaaatacttt tcattggtat atagcagctg gcgatagccg gtcccgtagg
    35521 cctcggagct gggaccgctg atgattccgt ccgacagacg caggccgtca tctttccctt
    35581 tcccccaagc gggacggaca aggtcgtacg aagcctccac gccgattccg gccttgagaa
    35641 tttcatccgg tgccgtataa ttgagcataa agcgggcaaa atattcccac tccgaccaaa
    35701 tccagccgat attttgcagg ttatcgcgat cattgacaat agaaccatcg tatttttcga
    35761 cattgtgaac atcaatcgaa ctaataccaa aggtcgattt gagggcaaat tcttcattca
    35821 gagctgtatt attttcagca gcaaattgga aatagcggaa acgggtctgg cgaaaatctt
    35881 cccattcatt gccgaatgga tatttaatgg ctgaattttg gacaagggaa gaggatgatg
    35941 aggcataccg cgcccagaag cgccacgtat ctttgagctg aacatcgata agggctttta
    36001 tctgcggctg gttgtcataa tcagacatat aagtggccgg aggattcggg ccgtacgggc
    36061 cgtccggctg gcccagatag ccgctgtggg acttatcaat ggaaaacaaa tcgggggtat
    36121 ggccggccgt atcagcaacg ctgacataac tataaacctt aaaagcttcg gtgctccagc
    36181 cccagctgag ataattttgg atgctgtcgt aacgatccca gaatgccccg ccgatggcca
    36241 agcccggatg ctgggcggcc tgtttcgtgt agatattaat gacaccgccg atagcaccgg
    36301 ggccgtatgt taccgaaccg gggccgcgaa caatttcaat ccgttcaata tcatttaaat
    36361 cccagttgag cagttccagc cgtgccccat agtgcgattt gatattgatg ttgatgccgt
    36421 tgatgttgac cagaaactta tacggccggt ctgcaatgac tccgcgaatg cccggcaccg
    36481 gcccgacact gtgattcata taaatagctc cgggcacata aatttcaatc aaatccagca
    36541 gattgcgggc cggagtcagc gcaatatcac gtgcagtaat aattgttacg ctggcgggag
    36601 ttttgagggg attggtttcg gttaatgttg caggcacata gacctcaaca tccatcagtt
    36661 ctgatagaga catatcaaaa agcttttctg tatccggcaa aacgctttca gcagcaaaaa
    36721 ccgtagtgaa cattaacagc ccgcataata ccgaacatgg atagctctgt ctcattttgg
    36781 ccgcatcatc catcttcagt agtcctcact tagtatatgg ttgaggttta tcgttataca
    36841 ctgctgtact gcattttatt gtcgcccaaa aattaaagat gcttaatctc tctttcatca
    36901 aattcttcaa aatgtattaa aaaaacctct caatcaagaa aagttaatgc cgggttgtgt
    36961 tgttttccgc aaaggccgtc cgataacaaa acgcagatat ctgtgataaa aaatggacaa
    37021 actgtccggt aaacatattt ttctcgtatc ttgtcaatca gcagcaaatt cgatacaatg
    37081 accgacaaat acaagtaata ttttatcctt tttatgtgtt aacaggagaa ataatgaaaa
    37141 tatcgttgga gtggctgcgg gattatatag atatagattt gccggcacag gccatagccg
    37201 atacattgag caatttgggc ttccctatcg agagcattga gcaggtcggt caagatacgg
    37261 tgctggatgt tgagattacc agcaaccggg gagattgcct cggacacatc gggattgccc
    37321 gggaactggc cgcagccaca ggtcagccgc taaagatacc tgctatcaaa ctgcaggaaa
    37381 gcccggagcc aacctgtctg aaggttcagg ttgaaatccg cgaaccgaca ctgtgcaacc
    37441 gctacacagc gcggattatc gaaaacgtca aggtaggtcc ggcgccagag tggatgcggc
    37501 ggcgtctgga ggctgtggga atccgcagca tcaacaatat cgtggatgtt accaactatg
    37561 tgatgatgga agccggccag ccgacgcatg catttgattt cagcaaaatc ggcgggcaga
    37621 aaatcgtcgt ccgtaaagct gtcaatggag agcggctggt aagcatcgac ggcacaaagt
    37681 gcgaactgga cagctcgatg ctggttattg ccgatgccga ccggccgaca gcaatggccg
    37741 gcgtcatggg cgggctggat tcggaagtat cggagcaaac aacaacgatt ttactggagg
    37801 cggctcactt tgaaccggtg tgcatccgcc ggaccgcccg tcggctgtcg cttcaatcgg
    37861 aagcgtcgtt ccgttttgag cggcaggtcg atacggacaa tctggcttgg gtttcaaacc
    37921 gctgcgccca gctgatggcc gagctttccg gcggcaggcc gacacagggc atcgtggatg
    37981 tctatccccg caaggccgaa cgcgacacca ttggaatgcg tcccagccgg cttcgtgccc
    38041 tgttgggcat tgagattccg caggccgatg tgctttctat tttttcgggc ttgggctttg
    38101 cacctgaagt tcgccatccc gatttgattg tatgtacacc gccgagctgg cggcatgatc
    38161 tttggcggga ggcggacctc attgaagaag cagcccgctg ctggggctat gacaaaattc
    38221 cggtggaatc gaaaatacat atcaaggtat gcaggccgaa tcttcgggag aaaactgccg
    38281 gctgtgtacg cacctttctg acaggagcag gcttttatga agccgttacc gtaagttttg
    38341 tcaatcagaa agcggcagag gtcttttccg gcaggccggc ggaggaacat ctttcggtat
    38401 ctgatgtttt tgggaaaaac acaaacctgc tcaggcagaa tctcatcggc tcgttggcac
    38461 tggtcttcca atccaaccgc cgcgcaggaa ataaaccctg ccggctgttt gagctggccg
    38521 atacctttat cccgcaggat aatcagccgg gtcagctgcc gcatgagcat ctgcatattg
    38581 ggcttattat ggacgatgaa ttccgcgcta tgcggggggt aattgaagga ctggtgcaga
    38641 aaatctgccc gacagctttg cttgatttca gacccagctc gctagcgtgg gcctcggcca
    38701 gcgcagagat tctgctcaac ggcaaaccgt ttggctgggc gggcctgttc agtcctgaaa
    38761 ctgcagctca atttgatatg gacaaagaga ttataacggc ggctgaactg gattttgagg
    38821 tgctgatgga atactcaggt tcagtcccga cagtccagcc cgtcccgcgg tttccagcta
    38881 ttgtgcgcga tttgtcgctg attgtagatg aatcggttac atgggcacag attacagcag
    38941 tggtgcagag ccgggcaccg gaggaattgg aaaaaattga ctttgcgggt ctgtaccggg
    39001 gcaaacccat ccctgacggc aagaagagcg ttaccgtttc gctacggttc cgcgatgaag
    39061 aaggaactct gcggcatgaa acagttgacg gctttcagaa cgagatactt ggcgccctga
    39121 tacaaactct gggggcagaa ctgcggacgg cataaccgcc gctggactaa actgcatagc
    39181 aggcggccaa ccgcttgaca ggatccgccg catacagaaa ggatttataa tggcaaaaga
    39241 agttgtgctg ccgcaattgg ggcagagcat gagagaaggg acgctgacgg cgattcgcgt
    39301 ggatgccggt cagtctgtta aggccggcga tattcttttt gagatagaaa ctgacaaggc
    39361 aacattggaa atggaatcgc cggaggaggg aaccgtcaaa gccgttctgg tagaagaagg
    39421 ccagactgtg ccggtttata cgcccctgct ggttctgggg gatccggacg aagaaattga
    39481 cccggcttat ctgacccagc tggaatggaa agttcaggca gccctgctca acagcacacc
    39541 tgcccagcag cagagtgccc cagccggctt gtcggctcac accttgcgaa ctgccgaagc
    39601 tgaacagcga tgcagaccgg cagaagaaat cccgccggct gcccttgggc agcagattcc
    39661 cttaacccgt ctgcagaaga tcatcgccga aaaaatggtc tggtccaaac agcatatacc
    39721 gtgtttttat ttaaatgtgc gggtcgatgc tacccgtctg gtcgaattgc ggaggcagtt
    39781 aaacgctgaa tcctctgtta aatattcctt caatgatttt atcatccggg cattggctgt
    39841 agggattggc cattacccga tcatgaccgg ccagctggca ggcgattgta ttcagctttc
    39901 agaccggatt gacatcggct tggcggttgc aattgacgaa ggcagcgttg cagcgccgat
    39961 tattaaggac tgcggcagca aaacattgga agaaatcagc cgagccagcc gagacctgat
    40021 agaccgtgcc catgccaata aattgtcgct ggacgacctg acaggcggct gcatcagcgt
    40081 cagcaatctg ggggcttatg gggtggattc atttattccg atagtcatcc ccggccagac
    40141 cagtattttg ggcattgggt cgctgcagga tatggttata ccggtcgata accgcccaac
    40201 aacccgaacc gtaatgaatt tgaccctctc ggttgaccac aaggtcgtca acggcgctga
    40261 ggccgcgcag tttctggact atgtcaaaaa acttctcgaa cgtccggagg agctggtgta
    40321 gttattcatt cggcaggtga taaatttcgc cgctgcgggc cagtatttgc ttttttttct
    40381 catccagacc gatttcttca tcagtcagat agcaggccgg atcaggcgcc caagggtcgc
    40441 cgtaatacaa atcagcccgt acccgcaaac tgccgccgca ggcatcgaag tagcggcata
    40501 accggcaccg ccctttgaca tagtctcgac ggcggcgcag ccccttcagc agcggctcct
    40561 gttgattgct ccagatttcg ctgaatggtt tttggcggat atccccgagg tcataatgca
    40621 tccagaattg gtcggggtgt actttgccgt tgaaatcaat gcagccgatc ccgacaccgc
    40681 tgctggccaa gcccccgccg ttccaggtca aaagcttcca gacctcgtca gcccttgcag
    40741 gatcttggac cagcagtttc aaatacagga aaacaccgtc ggcgtgatta tccaccgtca
    40801 gaatgtcggt ttttcggccg ccttgtgtca agacacgggt tttggccaaa atcatctcaa
    40861 ctgcctgccg tgtttggaaa gcggtcaggt catcgctata tatccgctgc cccctgccgg
    40921 ctggaacaag atgataaaag caggcccgtt caatcccctc tttttcgaag aaatgaaaca
    40981 gtccttccaa gtcctcaata ttccggcctg tcagagtcag ccgcaatccc acccgcacgc
    41041 cggcatcctt gcaatgccga atcccggcaa ctgctttttc gaacgccccc gccgcccccc
    41101 ggaagcggtc gttgctttct cctatgccgt ccagcgagat accgacataa gaaactccgg
    41161 catttttaat ctgccttgcc ttttgggcgt caatcagcgt gccgttggta gagaggacgg
    41221 cgtgcagtcc cttttgtact gcatagtcaa tcagttcaaa taaatcggcg cgcatcagcg
    41281 gttcgccgcc gctgaataaa acactcggca caccataggc ggccaagtca tccaacacag
    41341 ttttggcttc ctgcgtcgtt gccgcgcagt cggcagcatc ggtaccgctg ctgctgtagc
    41401 agtggacgca ccgcaggttg catcggctgg taatattcca gacgacgatg ggctttcgct
    41461 cagacgccgc cagtggaaca ctgtcgccgg cccgctgacc gccttcaatg ccgtatcgaa
    41521 gccagtcacc gggagtaagc tgtccgcagt aaagtttaga aatattaatc atggctgttc
    41581 tttcgcggcg gctggtaaat gcacatcggt tcggcttcca tataatcccc tgtcgctgca
    41641 aaagcccgtg cgcggcagcc cccgcaaact gttttaaaac agcacactcc gcatttgcct
    41701 ttcaattgcc ctgcatcctg cattctggcc aaatccggac tgctgtgcca tatatccgtc
    41761 agcgtgccat gcaggatgct gccgcagaaa accggcaggt agccgcaggg gaaaacctcc
    41821 cctttgtggc taacaaacaa aacgcctgat ccagccaagc atcctttgga catccgccgg
    41881 ggctgcggct tttcctgacg gataacccgc tgatagtggg gaacacaggt aactttcagc
    41941 tctatgccgg ctgtctcttc tttgcgggct atatgtctta acaactgctc gtattcttcg
    42001 gcagaaagca tttgctcaca gtccaattgc tgcccgcagc cgaccggcac aagaccaaac
    42061 acatgcaccg ccgccgcacc cagcgcgcaa gccagttcca aaactgcatc cagatgaccg
    42121 gcattatggc gggttagggt catattaatc tgaaacggca catcattgtg acgaagatgc
    42181 ctgatgccgc acacagccgc ctcaaaagat ccatcctgcc ggcgcagctt gttatgaatc
    42241 cgggcatctg ctccatccag actgaccgag acccgctgca cgccggcaga tttgatcttt
    42301 cgggcagcag cggcatcaat cagtgtccca ttggtggcca gcgcccgatg aataccttta
    42361 gccgccgcat attccagcag ctcgaaaaga tcttctcgac agagcggctc ccccccgctg
    42421 aatacaagca gcggcataaa ggcctgcgct tggcccagcg ccgccagctg gtccatcatc
    42481 tcctttgcct gcagcgtcgt caaatcagcc gtctgctctg catccagccg ccggcagtgg
    42541 atgcaggaca gattgcagcg ggcggtactt tcccagaaca ggagccggag ggtgcgggta
    42601 tagtctgctg cgtgctttgt cattggtttt tcttgatatc ctcagaaata atctgctggg
    42661 cattgtccaa aatgtgctga gcaaagcgct gggcctcttc gggaccatgc tcgcgggaca
    42721 gcaggtcgat attcttaatg acacagtggc acaatttgct gaccaccctt cggatggacg
    42781 cttccatcgt ctccctgcaa tgcgcttcct gacgggggcc ggcaaaaaac ttcctcattt
    42841 ccagttccga tatggcttcg aaggcctctt taatctggcc gataacaggg ccaacctgat
    42901 gtgtgccaaa ccaatccata aacgcactga ccgattcgta aatgattccc acagccagtt
    42961 ccagatcgcc ttcacggagt ttgatattgt cctgagcggc ctgtgccaaa tcatcgatgc
    43021 tgtacagata tacattttcg atatcaccga cagccggctc aaagctgcgg ggaacagtaa
    43081 tgtcaataat gagcagattt cgattgcgcc gctgctgcat caggggcttg atgcgttctt
    43141 ttgtgaacag atacccctga gcagcgcaag cggctcctac aacaatgccg gcttgggcaa
    43201 tttcctcttc cagatgctcc cacgccacaa ccgagcaatc atgcgtttcg gcaatcctgc
    43261 agctccgctg gggagaacgg ttaaccactg ttacatggcg gcatttttca tgctggaaat
    43321 gctcgaccag cagctgtccc atctgtcctg ctccgatgac gacaatcttg gccgactgaa
    43381 tatctgaaaa aagctgtttg gccaatccta cagcaactcc cgccacgctg acgcgccggc
    43441 tggcaatcga agtgtgcgaa atgatttgct tggccgtgcg aaaggcctcg tgaaacagat
    43501 gattgagaat cttgccggtg gtccggcagc tgcaggccag cttgtatgct tccttaacct
    43561 gtgcagtaat ctggctttcc ccgaggacca tgctgtcgag accggctgtt acagtaaaca
    43621 gatgcgccgc tgcttcttca ttggtcttga tgtaaaggca gggttgaatt tgctcaaaag
    43681 gcgctgaacg gaaatccgcc agccaccgag ccaattgttc ggccgtcgga cctgccgagc
    43741 ggctgacaac cgcgcagcat tcgacgcggt tgcatgtgga cagcaggaaa aattcagatt
    43801 cgctccaggc ggctttcagc cgacccagtg cttcagaagc ctgttcggca tcgcaggcaa
    43861 gctgctggcg aacatcaacc ggcgctgttt tatgattaag accgaccagt ataatctgca
    43921 taatgctatt caatcagact ctgcggattg gctggagcca tttcaaattg atgacgggat
    43981 gtatagaaca atctgctgcc gacaaatgca aacagaatca caagacacag aaaaaccgct
    44041 gcttgggctg ccgttttccc gctgaaggcc gacagatgcc gcaatacaag cacagcaagc
    44101 agtccgagcc acgcagcggc aatcagtaca accttgaaat ccgctgccca gtcccgcgct
    44161 gtcaagccca gctgtgccga tttaacagct gccagtccga tgccgctgac cagccccgct
    44221 gtcagagcgg caaaacccag ccaaagagcc gctaacatca gatgctccag cgtttctatg
    44281 gtaggcattt tccccaaaag ccccagaagc tgcttgcttt taagacgccg gcggctccat
    44341 agaaacagca atcccgctgc cccgcccaga acaatcatcg ccccggctat cgccatcgac
    44401 agggaatgaa ctatcaccaa aggagttcgc acggtttgat tcagcgaaga aaccggcctt
    44461 gccgctgccc aagccaacag cacaatcccc aaaaaagtcc atgccatcac cgacgaaaac
    44521 cagacccgac gcatcaaaga ccaaagacat aaaatgataa atccaataaa taccaaaaca
    44581 gccagtatag attcaaaaac accagtaagc ggaaatgcct caatagcagc agcccgcaag
    44641 cccaaaagca ttcctgccat agcaatctgc agcccaagca ggatgccgac ggcttccttt
    44701 attcgaccgg catcccgaat agtctgcaca tatgccagaa tggaggagac agcggaaaat
    44761 ccgatcagtc cgcctaaaag cagcttttct gaaagcatca attcggtcat aacactattc
    44821 tgcccgcatt gctctgtccg caatgcagtt atccacactt tatcctgtct taatgcttca
    44881 acattatcac ggtgtttgaa ggccgattat tcatctggaa ccaagcgggc ttattgcaca
    44941 gtaatgggtg actgcacttc ccgaccatat cgaccaacat actccgcatt aaagatcaac
    45001 aaataccaac tctttccggc tattattata atggaaaacc ggcgttattc gaaagtaaaa
    45061 accaaaacac aagtctgcac agaccttttt atctgctgtt taatagctgc gcagactttt
    45121 taagagggcc tttgcatgaa gaaattgcct gcttattctt cgactgtata gagaataata
    45181 ttatcttggt ctttgatgaa tattcgatta ccagcaatca ccggataggc atagataggt
    45241 gtctcagata cctgcacttg ggctatttcc tcatattgct ggccgttggg cttgtaaaca
    45301 agcatttgcc cgctgctcgg cagcgccagc agaaccgagc cggcatcgac aatttcccca
    45361 aaaccgctgc ggtcccggga tacagtatcc agccaatttg tcttgcccgt tgctgcatcg
    45421 agacagaaaa gattgccgcc gttggtaagc ccgaacagca gcccattctt cagcacaggg
    45481 gtattatact gtacagcaac ttcagaattg ctccacagtt ctacaggtgc aaaacctccg
    45541 tctttctttt caaccttcat tgcaaaagtc ccccgccctg caccggtata aatgacagtc
    45601 tggccgtcta taatgggggt agccgcatta taggcacgtc cctgcggtac aaacggaatc
    45661 tgccacagaa gctttccgtc ggccaaatca agtccaacaa tgctcttttc agtcagcgta
    45721 ataacctgct tgacaccgtc ggccgtcagc agaaccggag atgcataggc gggaccttcg
    45781 tcggcccacc gccatttttc ctcgccatca gccagcgcat aggcaattac agccccgctg
    45841 ccttctttgc ccagatgggc gacaaccttc ccgtcgacaa tcagcggcga cattgcagta
    45901 aaaaagcgtg gtaccgactg cgggaacggc tcttttcgcc acaccatttt gccggatgca
    45961 gcatccagac atgacaaaac gccgcccact cccagcgtga caacttttcc gtcgccaacc
    46021 gccagcgaac ttcgggggcc gggatggcgg gctgcagccc ctgtgacggc agaagccgtg
    46081 tattggtctt tccaaatttc acgccccgtt ccggcatcca gacagcgggt aatttcttga
    46141 tcgtcctgac gggtgaacac atagagctta tcccctacta aggcaggggt tgcatctccg
    46201 gaaccaaccg tcactttcca tttttccggc agcttttgag gccattgggc cggcggattg
    46261 aaatcgctga ctttggcatc tcggttcaga cctcgccact gcggccagtc ttgggccgaa
    46321 acagacattg cagcaagcag aataacaagc gtccctaaaa gatatgactt tttcatacga
    46381 aaaacctcca actcgctgta ttacaagccc gccgaactgc acccgtttgc agcttccaag
    46441 cggcagtctt accggttaaa ctctaccgac acagtgggaa tttgtcaatc ccattcggaa
    46501 caattaaaca gtagattctt aagcttgtat tattccgact ctatcaggcc gaaataatgt
    46561 aaacggactt taaaaactct gttctggtgt ttattgattt ccatttcgtc cttatttata
    46621 ggactatgaa gtatttaaac taagtcaaat taccataaaa aacagcattt cactcaattt
    46681 gctgatgggt caggcatttt ctaacgaaaa tcaaaatacc ttgtttgtat tgctttataa
    46741 agtaaattaa caaaaaaata tcttataata cttttattat ggaagatttt tataaatcgc
    46801 tattttcgca aatgccttcg gaacagttca ggcaggaggt tttcggtcta acagagccgg
    46861 aaggcccggt tttgattttt gttgatgacc gctatcaggc atatgcaaat gatccggaaa
    46921 gagcggcatt tctaaggaaa aatccggata ttctgcagta cctgtgtcgt cgaattgacg
    46981 acgggaatga tccttgtgtt ctgccgatga aggatggttg cacagccgga acacaactgg
    47041 ctgatgagcg ggggcactgc ggttattttc tgatttttct gcccggctat acgcggcaga
    47101 ctgcacaagc caatatggac cttattgagc tgattctcgc ccaagcccag ctcatttttt
    47161 gtttgattga aaagaatagc cggttctgcg gctgtcaaac aagcatttcg aatctcattt
    47221 ctccagagaa aaccgcaaac tgcctgtaaa accggggcgt tttatagggg aaaaaacggc
    47281 cttctgcttt ccaattgcct tataaagatt cctttgcaat ctccggcata tttgctataa
    47341 tccttacttg aattgtagaa catccaatca taaccattga tgcaaggggt tgttttgaaa
    47401 ttccagttat ttaaaaatgg aaaacctata gaagagtatt cgctggccgg ggcttatctt
    47461 tttggaaccg atatgattcc gcttcggcat gtcgataaaa tcgagtttaa ggatggggag
    47521 atagagtgca tcaagaaatc gcaggatgcg gcgggtcttt cactcctctg gccggttgaa
    47581 gacaacggca tgctgatgct caacaccacc cgccttccgg atcgggcaga accttataat
    47641 cttaatgtcg aactggcccg tgcccgaatg atgcagataa ccctgaaacg ggaagattgg
    47701 gcattatttg atcccagcga tgagctggag ggtatggctc aggaagtaca ggaccttttt
    47761 attcaggccc ttcagcacag cggagttccg gagcgggctt cgatgtttgc agatgaagcc
    47821 ctgaaaaaag gaattgaatt ctccgaaaaa ctggccgcca agcatgccga acaatacctt
    47881 gcagtgcggt ttcgcaacaa agctcttggc agacactcgc tggggtgcga gctgaattta
    47941 aatcaaatag cagacgaaaa ataccgcaaa tatcttctgg acatgttcgg tttcattaca
    48001 attcctgtca attgggcaga aattgaatca gaaaagggag aatatgactt ttccaaaatg
    48061 gatcagtgca tacagtattt atccggtcat cgtttggctt tgtgcgctgg tccgcttctg
    48121 cggttctcgc cggattcggt tcctgaatgg ctgctgaatg aaaaagcgga ttttgaaaaa
    48181 attcgcgaat acgcgtatga tttcgtcacc aaggtggttt ctcgatacag caaattcatc
    48241 cacgcttggc gggtcatcag cgggatgaat gcactgaatt gctttgggtt caattttgag
    48301 caggtcattg agatgacccg gactgcatgt cttgcggcta aggccgtgga tgtaaaaagc
    48361 cgcaagatgg ttgagctgct gtatccttgg ggtgagtact atgcacacga caaaaccact
    48421 gttccgccgc tggtgtatgc cgatatggtt attcaaagcg gcatcagctt tgatgcattc
    48481 ggcgttcaga ttcatttcgg ccgggatatt ccgggaatgc atgttcggga tatgatgcag
    48541 atttcttcgc gtctggattg ttttgcggcc gtgcccaaaa ccgtgcatat cactggagtt
    48601 tccatacccg atgctcacgg tccgggggac cggtcatcag aacgggcagg aagctggaga
    48661 aaaagctggg atcaatccct gcaggcatct tggattgagg agttttataa gctggcgctg
    48721 ggccgaccgt ttatcaacac ggtaacgtat tcgtgtcttg ctgactccag cggttcgcca
    48781 atgaacagct gcggtctgct gacggaaaat ctaacgccca aaaagtcctt tatggtgctg
    48841 gccaagttcc agagaacaat cctgcagcgc tgaattcgaa tgaacggact atgcggcaag
    48901 caccgagcca ttcagagtat ctccagaaga ttcacttgtg ggccaatggc tgtgcgtcag
    48961 cagcggcggc tggcctgctg gcaatgatgc tgccggctat ttgcctttat acagtgtaca
    49021 gaacgcctcc gctggaggcg cggccggagc gaatcaatcc aaacactgcc agtctggcca
    49081 gtctggtacg tctgcccggc atcggcaaag cccgggcgct ggacctgatt gcctaccgca
    49141 gcgctcagcc gcagccggct ttccggtccg ctaacgattt ggaagcaatc aagggcatcg
    49201 gtcccaaaac agctgaaatg ctggccccat ggctgacatt tgaagaagaa caagaactgc
    49261 aaatggacaa accagatacg acgcattttc ggtcagcagt tcctcaaaaa attctgctgt
    49321 accctacatt accggatata aagtaaagca taatgcgctt gcagtgcgac cgaaaagatg
    49381 ctgtactgta tccgccgcag aaatacaagt gaaacatgaa agcgaaaaat gagtaattgc
    49441 tatggttaga tgaacaggca ctttatgagc aataacaagg attttggaga ttgtcaaagg
    49501 attacgatag atcatggcag actgggaaat caaaaaacct cttgggcaat gcagcggcac
    49561 aggccggccg tttgaaatcg gcgaggaatt tttcgccgcg ctggtggaaa cgccgcaagg
    49621 acttatacgg caggatttca gtgccgccta ctggaatgaa caaaagccgg cggtgtactg
    49681 cttctggaaa agccggatgc ccaatccgga gcagaaaaaa cggctgttta tcgatgatga
    49741 aatgctaatg gccttttttg agcgtttggc cgaagagacc gagccggaaa agattcaatt
    49801 ccgctttgtg ctggcgctga ttctgatgcg caagcgccgg ctgaaatacg agggctgcat
    49861 attcgagaat ggacgcgagg tctggtctgt ccgcatcgcc ggtcaagacc gcatcgaaaa
    49921 agtaatgaat ccccggctga cagaggatca gattgagggg cttaccgctc agatgagcca
    49981 gattctgcag atggatatcg aaggaaacga atagaccaaa tcgggattgg acgcctcttg
    50041 ggctttatga atgaaagcaa tcggatttta atgcggattt agcattgggc aatcaattgc
    50101 ttcagcaaac cggccgcggg ggggctgtgg aggagcggat gacaagtttt tcatctaata
    50161 caatcgttac tgggctggcc tgctcgggat gttggatttg ctccagaagc agttcggctg
    50221 ctgttttgcc cattttttca gagcacaagt caataaatgt cagccgcggg ctcatcacac
    50281 ggttggcata ttcatcgcag aaacagatta tgctgagctg ctcgggaaca gcaacatgga
    50341 gcgaatgagc agcctgaagc aaatttagtg cgcccatatg gccgtatacc agcacggctg
    50401 ttgccccttt ttcatagaca attgtatgca agtaatcttc cgccgtatcc ggcatttcgg
    50461 cagcgctggt gatgggctct aacccatgct ggcgcatctg agataaatag gtttcatacc
    50521 gatcgcggag actgctgtgg caggtcagaa catctacttc aagcccgaaa ttgcgaaact
    50581 gaatcggact tcggtaatcc tccggcgcat cagtgcccgc ctcaaacggc ggcagcgggc
    50641 cggaataggc aatccgccgg tgccccagct caaacaaatg ctgcattgcc agttttgtgc
    50701 cgccgatatc atcgggcaca acagaaaaac catcctcagg acaagggcca tttacaacta
    50761 cataggggat tctcatctgg acaatttcat ccatcgtaac cgaggtacac ttggccagaa
    50821 tcacagcacc atcaattttc caagccggca tttcgctgac atcggtggaa tgacgggaca
    50881 tatccactgt tacatcgtaa tcttgggata cagattccat cacctgtgta atattgttca
    50941 caacagtctg ataaatattt cccaaatcgc ttagggcgtg gttgccgccg aagatatgaa
    51001 tcatccgcgt agactgcatt gtcaggcttt tggcaaacag attaggccga taattgagct
    51061 tgcgtaccgt atcaagtacc cgctgccgca cctcatcccg caccgaaaag ccgttgccca
    51121 gacgattatt taaaattcgg ctgactgttg atttgctgac acctgctatc cgtgcgacat
    51181 cttccagact cggaattccg tttttcataa gattgtacta ataaatgagt tagtattgtt
    51241 tagcaaccga ttgctctatt tttgtattat atacgtcgcc aattcgaaat tcaagcaaaa
    51301 tgttgctaac acgcccctcc ccttacaaaa acaatttaga taaactgtca atcgagtcat
    51361 tattggatct ttaatattca aaataatcat taataatttg ggattgcttg aaaaactgag
    51421 aaataaggca gggcagacaa tttgctcagt ctttatggaa tcgcgcgatt ctggggattg
    51481 cagaaccaaa tcatatcatt catactttta tccccatcca gagagtcagg aggattgtag
    51541 taaaaaagta tctccttctt tcgaaagtcg atatgaccgt caaaataagc ggtatttgct
    51601 ccttgactgt gacgaaaata aaccggatac cacatattcc gactgcggta tctaacaata
    51661 ttttgcccat ctctgtccca ataaagccgg taattagccc ccggctggta tgctgcccag
    51721 tctccggcat ctacaaacat aatcttgccg gccggcgacg gaatagtact gatgcgaaac
    51781 cggcatgacc aattcgaaga ggggatattg ccggcccaga cagctggact cttggaggta
    51841 ggcccccagt ctgtaaaatt atagccgtag ctgacataat tctgataaac agcccccgtc
    51901 tgctggagcc aataatcttc atctgcctgc tggtccgcgg gacagaggta ttcttcgggc
    51961 atcacaaaac ggccgccggc ggaagcctct tccagcccga caatgctgcg aaattccgcg
    52021 ttggagttcc atgtcggttc gccgcgggct gtcattgtgg tatctatcag agggacatac
    52081 caattgtccc atttagcggc gtaaatcgcg ttggacaaag ccagtgtttt aaggttggtt
    52141 tggcagacaa tgcgctgggc atagagtttg gccttcttca atgccggcag aataaccgac
    52201 agcagcaatg caatgacagc aatgaccacc agcagctcaa taagcgtaac cgccgctgct
    52261 tgggagcctt tgatggattt tctgaatccg ccggtcatcg tctggccctt ataaaaaaaa
    52321 aggccgggtt aacaagcccg gcctttttgc agtattgctt tatcgatatt atctccgggc
    52381 cttccttcct acggtgtaca gccaagcttg tcgcttaagc agtccagcca agtagccgcc
    52441 agaatcgcaa agtcagccat attgactaag cagtcgttgt tgacatcgcc gatgtagggg
    52501 tatgtatgat aatggtcggt cggatcgccc tgcgctgctt cgcaggcgct gccgtacaca
    52561 atcacttcca caatatcctc ggcatgggcg taggcatcat ccgcgctcag acggaagcga
    52621 taaaagcccg ttccatttat cgtaacaacg gagtctacct gcgtcggcgt ggcaatattc
    52681 accacagtcg ccgggtcctg ttcggaataa atcaactccc acaacagtga caccggactc
    52741 ttgccgtcat ccgtcagtgt accatccagc ataaaagtta ccttggtcgg gtcgccgtcg
    52801 ccgtcagcca tcgacagcca caggtactta ttggagccgg catcaaccac cggcggatcg
    52861 tcatccagcg tcgtgaaggt ccatattggt ccctgcgtca gctgcggaac gccgttattg
    52921 ggatccgttg aattgacgcg ccaatagtat tttgtacttg gctgcagaaa aatcccaagg
    52981 gaagacagat taacagaatt ggttgtcagc cctacaacaa tcggtgcaga ttggaaattt
    53041 ggatccccat catctttttc aagataaacg gttgaatgaa tggattcagc cggattatag
    53101 ggcagcggat tggtccatcg aagcacattc gtcgaaggcg caacaatctc cccattataa
    53161 ggatggggga attttgcctg tttggaaatt ccgaccggcc ctaaatatat atcgtcgtag
    53221 aaaacaattc cgctgggcgg ggtgccggta ttggccatca ttaaattgag cgtgagggtc
    53281 gtcgcgtaag gaatggaaga gttatcgatg atgccggact taaagtacca tgtattaaca
    53341 gcatcgccgg gcacaatggc caaatctact tcagaaggac caaaactcgc tcggatgacg
    53401 ccgttgcggt tgaccattcc ctgcgtggtc attattttgc cgccgaattc atactttcct
    53461 tggccaatcg ggatagtttg gaaaatcccg tcatcatgcc accacacacc cgcacagtgg
    53521 ttggccggat tcagcggatc agtaacatag cggttggagt tgccccccca ccacacactc
    53581 caccctgtcc ctccgggaag ctgctcaaag tcgccattga tgatgagatt atctgcccaa
    53641 actgtccaaa ctgccgcaat taccattaaa attaatgaaa ctttcctgct catcggaacc
    53701 tcccataaat attgttgtgt tttattctca cataaaaaca cctttcttat tatatttttt
    53761 attcgacata aagcaagaat attcttctta aaaaacataa aatacagtga tagcaaccga
    53821 ttgctaatta ttcctcataa agggcattca atacctgatt ggccatttca acaagaatgt
    53881 ctaattgatg taacatcttt tctaaaaaaa gcttacgata gatataaata gtccaataat
    53941 acttcttata agaaatatgc tatatcccct atcctgccaa ttataaaaca gcaattgccg
    54001 tctccgtatt ggacactaag aatcaacatc tgctgttttt agaaaacgcg ttttaaatta
    54061 aattatcgca attccccttg acttaattct cttaaaagga tataattcat tgcaagaaac
    54121 cggttgctaa tttgccgaga aatagttagt gtttacggct ggagtgagag gaaagatgac
    54181 cgatacaact cttaaacagt gcagtgcggc gagatatgtc tgcccgcatt ttaaccattc
    54241 gaccaaattt atccctcaat taaaacaatc aatcaaagga tactttgtgt aagcagaaaa
    54301 ttactaacct attttggagg aaagaagatg aaaagagtaa tgattttgct gtcagttttg
    54361 gcaactgcgg gattggcaac tgcaaatctg ttgaccaatg gcgactttga agaccctgca
    54421 ggttcaggat ggtctcagtg gtggggcggc aattccaata aatatgcccc tgatccgatt
    54481 gaaggcgacc actgtgcagg cgtttggtgg atggatgacg gaatcttcca gaccattcct
    54541 atcggcgcgg cagtttacac cgtcagcggc aacattatgc aggagaatct cggaaacgga
    54601 cgaatcggcg tcatcaaggc cgaaatcaaa gacggtgccg gcaatatctg gtggacacaa
    54661 gaaattccta taactcaaga tgatccagcc gggacatggc tgagcggctc tatgaccata
    54721 gacaattcca ccgcgggtgc ctccctgatg acaatcaatc tgtttatgtg ggataaaaac
    54781 ggctggggaa gcggggcagg cattgtccgc tgggataata tttccgttgt cccagagccg
    54841 gccacgctgg ctctgctggg gcttggcggt ctgctgctta gacgccgcaa ataaaaattg
    54901 cttctctgtg tgatggaaaa tgaaaggcgc agcttttgct tgaggctgcg cctttttgct
    54961 tgctgcattt gctaaacagg gtacaaaggg aatatgcaaa agttcctgat tgttgttctt
    55021 ggcggcttca tctgcagttc atgtcctgct gctggggtca atttgctgac caacggaaat
    55081 tttgaatccg gcggtattgg ctggtcgcaa tggtggggcg gcaattccag tgtaaatgca
    55141 gccgaccctc tcgaaggcga ccgctgcgca ggggtgtggt ggcatgacga tgggatttat
    55201 caaaaccttg tcttagatgc aggaacttat caattcggcg gcaaactaat gacaacccag
    55261 acaggcggct taaaagaccg acgcatcgtc attcaggttc aggccggcgg cggctctccg
    55321 acccagcagc tccagattgg tcccggcgat tcggaaaata tatggcattc cgtatccggt
    55381 tttatcactc tgacagctcc tgcttctgta acggttacat tgatgcttgt cagcgacttg
    55441 gggtccaatc ccagcggtat cggccttttt gatgacattt acttaagcga ggtcgcccag
    55501 c
//
LOCUS       DAOTBO010000006        55407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_478_length_55407_cov_5.889597, whole genome shotgun sequence.
ACCESSION   DAOTBO010000006 DAOTBO010000000
VERSION     DAOTBO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 55407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55407
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_55407_cov_5.889597"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLE88_01435"
     CDS             complement(<1..495)
                     /locus_tag="PLE88_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75159.1"
                     /translation="MIKLPAILAVGVKLSPLDVWVFVLYLFILLSIGAFVSYRQRNSE
                     DLFLGGRSMGWANVGLSIFGTNIGPTFLIATCGAGYTTGMVTANFEWMAWIFLFLLGM
                     VFVPFYLNTKIATMPEFMNKRFGPGCYTFMSFYALLGTVVLWIGGTLFAGGALLSQLL
                     GWKLM"
     gene            complement(488..1285)
                     /locus_tag="PLE88_01440"
     CDS             complement(488..1285)
                     /locus_tag="PLE88_01440"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOM75160.1"
                     /translation="MKKLSWLVLFPLLFSFRTYAFSPYENYLLSGRPTHVGLIARAKS
                     GQDDKLSQKLLELCNDQESEKIKQAGISNLAVFKRQIQSETWHVVYFQYSGGKQYLKA
                     AQSFEAITPELKTLINPHPRAVRYGCSWLQMEWINYIRGLDREGRPTQILSMITTIKP
                     EKESYYRSLHQTVWPGVVDQMVRGNNRNFSIFLVEIGDTLYELFYLEYMGSDFEKDDQ
                     MNREDPVNQRWWAQTDSCQQALPGEKDIWTPMVQVAIASLQKDSAHD"
     gene            complement(1312..3657)
                     /locus_tag="PLE88_01445"
     CDS             complement(1312..3657)
                     /locus_tag="PLE88_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOM75161.1"
                     /translation="MHLTKQPEKYYGGDPWTVVEEGFQAEKQRLSESIFSVSNEYMGV
                     RGYFEEGYSGDHLLGSYFNHLYDYLEIGHDQLFKGMVDKSGAMINAVDWLYTRIRIDG
                     ELLDLAAVCFSDFRRILDMKKLVYTRQFVWHLRSGRQVKITFIRFLSMHMTKIGCQRI
                     LLEPINFSGKADIVLGLDFNSRYEIASGWTQTQTTGSKDSDRGKNFWNEVKKDSDDTS
                     CRILAQTKTTGFQLFSSFRMLTAQPVAPHVLEHDKFIGLGFSLSLDKNKAACIDKIVF
                     NHWEKNDHTDENWKNACKLAAGYADVTFEAAYKQHVQFMEHVWDRFGVAIDGDDDVLQ
                     GLRFSILSCYQTYHGENPHLNPLCKGMTGEVYFGWAFWDTEIYCHRFHLFVNPQAAKN
                     LLMYRYHTLDHARRRARDLDCRGARYPFATVSGYEDSGTWQHVDLEHHISGAVGYAIW
                     QYDKITQDKDFLYREGIEMLLEISRFFASAGDYSPSNGDFGLYGVMGPDEFHMMVNHN
                     FYTNYLAKKIFLYTLDVLEEMKANAPEQYKAAVQKVALDPAEPKAWTDMAQKMRIRQD
                     PDTKIFEQHAGYFDLPHVEIADIPITDIPIYKNWAYIRIFRKNMIKQPDVLNHMFFFS
                     EDFTLEEKKANYEFYESRTIHESSLSPSLHSILALEVGRSDQAYDFFKYAARLDLDNY
                     NRNTEQGLHATSASGVWANLVCGYGGMRSDGSVLSFWPRIAKNWKGYQFNVNYRGGVI
                     AVSVDKTKAVFNLIEGVPVKIKIYGRLYQIDPKGVQLPLIC"
     gene            4044..6764
                     /locus_tag="PLE88_01450"
     CDS             4044..6764
                     /locus_tag="PLE88_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75162.1"
                     /translation="MNQVICIVLLFFVISPTTAMSSESRSLAVHTNNNTEESQTSPHQ
                     LQSPAGLTVEVRGQTSPRLTYASGRLQQHLRAFGPARIILSSHNLNKVEDRRNPEGFY
                     LYRDAEGVFHIEGYGDSGALYGALELIDRVKETGQWPATLEVTEAPDFQLRGPCIGMQ
                     LTSILPGRGTYEYPYTPENFPFFYDKDQWIEFLDFLVANRLNTLYLWNGHPFASLVKL
                     EDYPYALEVSEEVYQRNVEIYRLLTEEADKRGIWIIQMFYNIFVSKPFAEHHHIETQH
                     AAPNELIADYNRKSISQFIKMYPNVGLLVCLGEALKGQENQEAWMNETIIPAVKQGLA
                     ALGKTEEPPIVVRAHAVSNVKQMVESALKHYTNLYTMAKYNGESLTTYEPRGQWQQVH
                     LDMSRLGSTHVVNVHLLSNLEPFRYGATDFIRRCVLACRDRLGAQGVHLYPLAYWDWP
                     NTPDKVNPYLKQYKRDWLWFEAWARYLWRLDRDPVQDQQYWIRRLTEMYGNPEAAELI
                     LKAYNESGYCAPLLLRRFGITEGNRQTFSLGMFLDQLVNPGPYGPISGLWEWHAPAGE
                     RLEEYAKKEWAGESHSGETPVGVIEEATGHAQSAVKAIDEAASRITRNQDEFDRLCND
                     IHCIDLISKFYAEKVRAAILVLDYNYSRNSESMRKAQKHLEQSLEHYRTLTERTSKTY
                     RYANTLQTGHRRIPIRGIKDGLPYYYHWEHMLPLYEKELEEFKNQLSHLDVTTPQPRR
                     AGPKLKSVPFELISQDCETYEVKVGAKPFTDRDYQILQIAEELEGLVGIRFSHTAGKA
                     GQLKPIRLKTSVPVQILIGYFNENRPIWAQPPNLDIDSQAAQYGGVESTMQNALQIEG
                     CPNVNIHVFSYPAGEIVFDPRSPGSYLFLGVIPADMEITSRDAGIAPGRM"
     gene            complement(6819..8411)
                     /locus_tag="PLE88_01455"
     CDS             complement(6819..8411)
                     /locus_tag="PLE88_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOM75163.1"
                     /translation="MPRETLHWIDWLIILFYLGLIIYLGKKFSEKGKSTDSYFVGSRT
                     IPAWAIGMSVLATLISSVTFLAYPGQGFSGNWILLVQGLAVPITLLTFVWLIVPLYRR
                     FIGLSAYEYFEKRFGFFARLYSSMGFIMMHFTKMGTVFYLLAMALASMTGQNTYGIVA
                     AVGIVTILYTLAGGIEGVIWMDVIQGLILIAGGIASCLILLLHSAQGPVPVLQTAWES
                     GKMSIGEFTWDFTRLTFFVMFINGIFYAIQKYGTDQTIVQRFLVAQTDKKAIKAALVG
                     TFLCVPVWTLFIFIGTLLWVYYRRRPELLAQGITGDQVFPYFIMTQMPVGLTGLIVSA
                     LFSAAMSTLDSDLNCLAAVGVEDYYKRFKKNADDQRCLHLGKMIVLTCGIISVGIACL
                     YIHLGGEAILGTLFELYAIFSGGIAGIFLLAFFTVRANRKGLNIGITACIVFTAYAVL
                     TSTKFDLGGSEKQLILDLGRYNFPHHKYMLGVYSHLILFVVGYLASFFFKPDKEVRQM
                     TVYGWIERQKNIPPDLAEKSKT"
     gene            complement(8401..9585)
                     /locus_tag="PLE88_01460"
     CDS             complement(8401..9585)
                     /locus_tag="PLE88_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOM75164.1"
                     /translation="MIVVVADDLTGAAEIAGIGCGCGMTAELQRGFCSKTDAQLLVID
                     TDTRSLSPQQAGQKIRHLAQGLNASGLPIEWIYKKTDSVLRGPVGCELETLREEMHAA
                     RVLLVPANPSKGRIVRDGIYLIRNQLLSETEFARDPEYPAATSNVLKMQGLFGFDQVC
                     CLDTHQPFPNEGIIIGQAETTDDLMRWAQRIDEQTLPAGAGEYFEAILKNKGFSKKPH
                     VDEIDYQLNTKTLYVCGSSSNASRQALRKAESLGMKVCRMPAGLFRPDCDEQNTFLQW
                     TEEVIGSLQYSSRVIVAIDREIVPDVAVRLRGLTAKLVQAVICEIPLSELWVEGGATA
                     SAIADSFGWVRFAPCFQLEPGIVRMKVMNADNVYLTAKPGSYAWPAGILNFFSKGFYC
                     AP"
     gene            complement(9609..10634)
                     /gene="pdxA"
                     /locus_tag="PLE88_01465"
     CDS             complement(9609..10634)
                     /gene="pdxA"
                     /locus_tag="PLE88_01465"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOM75165.1"
                     /translation="MTERRPILGITMGDPAGIGPEIAVKALADKNVYTIAKPIVVGDA
                     SVIARATQIAGVSLQVRAVSNIGEALFQYGTADVLDLNNVDTGRLEYGKVCPMAGKAA
                     FEAVEKAIMLAMSGQIDATVTGPINKESLNKAGYPYSGHTEIFAAYTKTRQYAMCLIE
                     GNMRVVHVSTHVSLKRACELVKTERILEVIKLAHDLCKRLGIEQPAVGVAGLNPHAGE
                     GGLFGDEEAKEIMPAIETARCFGIDADGPVPADTLFSKVKGGQYDIAVAMYHDQGHIP
                     VKVTGFVLNHKSKKWESVKGVNITLGLPIIRTSVDHGTAFDQAGKGTANEESLILAIE
                     YAAKLAV"
     gene            complement(10653..11573)
                     /locus_tag="PLE88_01470"
     CDS             complement(10653..11573)
                     /locus_tag="PLE88_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOM75166.1"
                     /translation="MSNGVKCQGVIVPMATPFTLEGHIDAKAVHRIVEHLIGGGVDGI
                     FVLGTTGEAASISLAEKVKMVQITADCVDNRALVYAGISSNCFNESITMARDFKDFGA
                     DIMVAHPPYYYSLNAGELFNYFIKLADSIATPLMIYNIPQTTGVSLPIDIVEKLSRHK
                     WIVGLKDSENSPGRLEAAVERFAHRSDFSYVVGCAALSAKALSMGAAGIVPSAANLQP
                     GLYKKLYENALTGSVSAAQELQKQTDQISALYQGPRSLGQSLAALKAAMHLLGLCEPT
                     VLPPLQTVQKHEIAEIQQQMAKLCINTVIS"
     gene            complement(11707..13791)
                     /locus_tag="PLE88_01475"
     CDS             complement(11707..13791)
                     /locus_tag="PLE88_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75167.1"
                     /translation="MRRIIYILICICMASGVSALSITNGDFEALGVREDVPYWFDYNV
                     GTGAAGTYLRLLNSPNGTQYMGLADGNSWAYQSIGFNTERLPALQIQYDIGRTTNAAE
                     PRDLVITVSIYESDGSFAGSDGPRSSDIDGVKGVKLIDSMRVAYPAVAPGQVITDSVT
                     LHYLDSTVGELFLRFRNNGDGNSDTEDLAVIDNVQIAELVSVVNPLPKNGTNYVAVER
                     TSGQNDLIFTVADSEIIKIDVLFGPDPNLSEAHKIVNGLSVSPGQYAITLESELSQDL
                     AYSTDYFWKVLAYEPNGIELSLKYQSPAWSFRTRQKGPYLGDVDPYKLGVHPGQDAVF
                     TVEYYNNTDSFQWYKAGVGALTNGAKYAGVNTDTLTVRDAQLADAGLYYCVGTETATG
                     LTAQTLTSGELKIKQLMTYYPFETTYNAGGKIYTPDIVSGKDMELKNGASLSDAYSLV
                     GKNLWLDNPKNQYAQIADNTVAHYDDITISFWCYATTLVNTTDGDRWARVFDFGKDST
                     EYIFFTLLYRQDDPRQNGLDVARCELRYYDAQGVAHNPELDVEHPDIAAGRWYFVAIT
                     IEADEDGQGGTGRIYFGGEHGTETETPQIMNIPKMTEITKTLHYIGKNIGFTAPLFNG
                     RIDEFKIYNYARTKEEIAQEYYGITGDASVCDMENYDLYDYDYDRNCVVDLSDFAKIA
                     VRWLDDYRIYAE"
     gene            complement(13835..15946)
                     /locus_tag="PLE88_01480"
     CDS             complement(13835..15946)
                     /locus_tag="PLE88_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75168.1"
                     /translation="MRRILCIIICLSVVPIASALSITNGNFENQTGLGSNYDIKYWYD
                     YGGPTLATTAGPFWNPANSFSMNRTKIACNGQGSNTSEDGGNHAYIYQSIGIYDGNPS
                     VAEIALDWGLIPTYTPGEMGITVMILESDGTFEPNEWDGAAHDIYGFRKTEANPNAPI
                     TEIGRGSVIRYAAEGVVMHERFQIDLTSATVGKELFLRINAFRRNVTPWVSFDNITLS
                     PAYVVNKSPQNGAVYVATELASPENDLVFNVADPAIVAVDILFGTDPNLITATKIVAG
                     MPVTPGLNTVDLVGELAQDLNWTTTYYWKVLAYESNGSGGLSLKYTGLITSFTTIQYG
                     PLLGGITPAIQAVWPGEDAVFTVPFTLKADTLQWYKAGNPDVALADGADYAGTSTNKL
                     TVLNCRTEDEGIYYCIGTETATGATIESTNRGELAIKELKSYLPFDSTGSDAQGMYSP
                     DIISGKHLYLRGGASVKSGADPNSIAGGHLKLLNPRGVTHTQYGEFLNNSIAHHRDIT
                     ISCWVKPTILDLDYERYARIFDFGQDANNYFYLTLLQATNAARCVMNVAGDDNELNGT
                     GDIGYGAKWIYVVLTIEDSDGKLYINGQYSGGGGMYSPSELPKTLNYIGKAIAAPETG
                     SYPNFDGLIDEVKIYNYARTAEQIAQEYMDVRTDVSFICDREDYDLGDWDFNGDCLVD
                     IKDLLVIAEKWLMNYFMNLES"
     gene            complement(16257..17150)
                     /locus_tag="PLE88_01485"
     CDS             complement(16257..17150)
                     /locus_tag="PLE88_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75169.1"
                     /translation="MKKLLVLVGLAAVVMLAADSQAFRVTTADPGGADAELREETPTT
                     NRGTSTEIASRIYASTDPTKWRNSVIYLKFGVAGITAQDLAGDITIRLTYRNTNLVIS
                     RIQDTVEPIGPNTGWDYFILDPTIDGANWDELTITPSNAPGYSLDNDYATKATGSPIA
                     PTDGLYYLGRQLYDSAALVGTPAHLPVGGAFDFTLPAGSLLHEAILMAQETDHKTLTI
                     VMGIAHDYDNDNTNWLNFNYLFNPKEMTTLLKDTASPWSEKSNASNLFSPMLTNEPHI
                     PEPATMALLGFGGLALLRKRK"
     gene            17898..18653
                     /locus_tag="PLE88_01490"
     CDS             17898..18653
                     /locus_tag="PLE88_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HOM75170.1"
                     /translation="MSRCINLTAVNAIMLVFISTLQAETAIEVAVKTQAAIDAGAAPA
                     WMSPNLPEDIQQNVQNKGKELAQSLKLGNEIKQSKVADLLAEHYGRVWAWHQQADEKL
                     DAAWLAWDDARDPAKGGKNELKALTIMVEQIEPIYAEFAPQIQGLLTALRKEITDEQI
                     IALLDRITRSPGVDRTYNAYLEMVPEMKEEEKKILRNRLEQARIESLSAWSDKSIVKI
                     FKKYKIRNEFSIDDFGYDYRKRYEAWVTRTKSK"
     gene            complement(18815..21526)
                     /locus_tag="PLE88_01495"
     CDS             complement(18815..21526)
                     /locus_tag="PLE88_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOM75171.1"
                     /translation="MSTHKIISDMKENVRCIIKRWFSGKNGRISLMQCNHLFVSIILV
                     FFPLLVYRAGADPGPMVLFYNSPASYTSDVTSNEALPIGNGKLAAMVYGGTGTEIIQF
                     NEDTVWAGQPHNYAHAGAVNVLELIRQHVWAGEGLAAYADASGSFMSVPLRQCPYQPT
                     AELRLTFNHSAPTNYRRQLDLSTATASVSYVTGGVTYQRDCFASYPDNVIVVRLTASQ
                     FGKINFTCALTTPHTVVSNSASGSDVVLRGAVDHVGKNGLASNIEFEARVRILCEGGS
                     IAVIGNTLQVSGADAATLVLGAASNFVRYDDISGNPSLICQQTVTKAAAKGFNALRQD
                     QLNDYQSLFNRVTLDLGTSNKVNLPINERLDAIEAGVDAAKYDWSLFNADDLQLIALN
                     FQMARYLMISGSRPGSQPLNLQGKWNNEFEPSWEGKMTLNINEEMNYWAAEVANLPEC
                     HEPLFDMIRDLSETGAAVAAAHYGADGWVVHHNTDLWRGAAPINNAGGLWPTGGAWLS
                     MHLWWHYEYSGDAAFLAQVYPLMKGAAEFFAGFLVRDPRPGSAGYLLTNPSHSPEQPN
                     PALGDNGELVAGPTMDCQLIRCLFTYVIEASKILDADAAFRQQLEQMRAQLPPNQIGR
                     YGQLQEWLEDVDVPNTHRHLSHLVDLMPAGNITPHHTPELAAAVKVVLDWKGDDTNNT
                     AWSQAWKMCCRTRLLQGNHAYMILNKILGKSHTDNMTFSRKGNENQIDGNLGVLMGTA
                     EFFLQSHQGEVYLLPALPDKMANGSVTGLRARGGFEVDITWQNHQLLTATIRSLAGKT
                     CRIRSAWPIVVSEAAHRVAVHSPGKDLYEFPTEAGHTYTLTVYSCSTPIASDLDGNCQ
                     VNISDFAVLAEAWMGEGDNTGYDLGDLENLAEDWLTCNRLPTSECWQ"
     gene            complement(21600..24623)
                     /locus_tag="PLE88_01500"
     CDS             complement(21600..24623)
                     /locus_tag="PLE88_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75172.1"
                     /translation="MIMLGNHHLTGVGYLFAVLLSLRSLSFGTELIGWATAAGGTTGG
                     QGGQVVTVTNKAEFISAVEGNTPRIIQVVGTIHGDYNIPNVGANKTIVGIGYDARIAG
                     FSVKVTDVDNVIIRNITFQGGMPQDGLICRRATHLWIDHCTFLDAADGLLDITDQCDY
                     VTVSWCRFYYTSKINPHRFACLVGSTDDNPTDAGKLNVTFHHNWWGRYVDQRMPRGRY
                     GSDHVFNNYYSCTGNSYCIGGSWGFKVLLESNYFDHVNNPMRDAGIVNVGSGGTYTVE
                     IKSVGNIFDGCTGSMQTYGQAFVPPYPYTLDAAENIPSIVKEGAGTTILVGATTLWPT
                     KATVPAPASGDNNASADAILCWRAGSTAVSHHIYVGASPDGLASCGIQAATSFTPPAL
                     KPDTRYYWRIDEITADGSVIGGDLWTFKTSPELPASLLHYWLFDVDFLDVAKVFASNP
                     NNPFGAAWLDTDVKLLGVGCLDLTYPKDGLIAGWSESATNTIFPPAAPMTISLWFKPT
                     VLPSSDNTACILGSKPGTVSGAKMFRIELLPSGVCRLTVDNATPEFGNLPVVNGWNHV
                     LITIGTSGYLRAWLNANEAGSISLLTNASNNYNGQYAGIGFYGDTPNGLHRGDYTGFI
                     DDVAVYNVPYDLTFAEILYNSGNGRAAIGNPVFTADPIGNQDAVELVPYVGQSLAAYT
                     GGIGVFSKESGPAWLAVLPNGMLSGIPSDADVGQNTFRVRFGNSSGAYDTAAMTINVR
                     NMYSGIKGMEDLAGLASEWLLAGCEDIPACGGADLDGDRNVSLPDLQILTDNWLADED
                     LVLYLPFDEMSGDNSADGSLYARTAGLVNGPTWVTGVSGGALAFDGIDDYVEIAGYKG
                     VSCGHMRTVCAWIQTSQISEMILSYGGLNPGTRWLFFITNSKLQLGVGGGNIFSNRSV
                     NDGQWHHVAAVLQEPASGPASVQNLRLYIDGQLDAGTYTNPTVEINTGTDCSVRIGVM
                     QKPDGALESFFGGQIDEVRIYDRALSESEIQSLLQ"
     gene            complement(24645..25754)
                     /locus_tag="PLE88_01505"
     CDS             complement(24645..25754)
                     /locus_tag="PLE88_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75173.1"
                     /translation="MAQTAAVDPVRMEAIYNEVKTPYKYGVVIHRDTEQNRNIDCPTV
                     FRHGDYWYMMHVQSEKDRAGYTTQLARSADLLHWERLGTILPQGNLGEWDYANAAGGI
                     ALADTEWGGKGHLGIYEGKYWLSYFGGAITGYEIAPLSIGRASATEPSAVVYWTKDKS
                     PMLSVTDPDAGAWEDYTLYKSFIFEDPNRTLGPDARFIIFYNAKGGSSESEFIGLATS
                     SDMRNWKRHKNNPVIVNGGPTHKISGDPQIVRINGIWVMFYFGAFWDPYPGNKSAAFD
                     TFACSYDLVHWTKWDGPNLIEPSTSYDKTFAHKPWLIKHNGIVYHFYCAVGDQGRVIA
                     LATSVDLRQEKTMSNSLLQQNRPTANDASYSSDSQ"
     gene            complement(26276..27772)
                     /gene="glpK"
                     /locus_tag="PLE88_01510"
     CDS             complement(26276..27772)
                     /gene="glpK"
                     /locus_tag="PLE88_01510"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOM75174.1"
                     /translation="MKNPYILAIDQGTSGTKAVLFDGQGQIAAKASSPLRSYYPRPGF
                     VEQDPEEIYESVLDAARQCIRQFTAQNGKISDIVACGISNQRETFLLWDNTGRPLCPA
                     VVWQCKRSVDICEHLKGSAVEKEIIAKTGLLADPYFSGTKLIWLYENNPRIRDAVASG
                     NAYFGTVDTWLLFKLTNGTQYCTDYTNASRTLFFNIHTLRWDLDLLKHLSLTGLNLPE
                     ARPSAYLYGASDFQGLFQKAVKISAMIGDSHAAAMGEGCFDVGTAKATMGTGCSILLN
                     TGSKPVQSQYGMVTTICWSAQDRVDYALEGVIVTCGAAIKWIRDQLGLFGDSRETEAM
                     ARAVPDSNGIAFIPAFSGLGTPYWRMDLKAAIAGLTLGSDKRHVVRAALESIPIQIKD
                     VIDAMQADSGIQLAGLNVDGGITANRFVMQFLADLLNVQVTNIGMEEVSALGAAYLAG
                     LQEGVFSNLAVLAKLNQDKTVFSPVQDRMYASDAYKEYQKYLHILLKA"
     gene            complement(27796..28746)
                     /locus_tag="PLE88_01515"
     CDS             complement(27796..28746)
                     /locus_tag="PLE88_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOM75175.1"
                     /translation="MIQQQRYDLRCVMVQTFIEAVENKTNLAIVVSDSTSTSKIEPFQ
                     ERFPERLINVGIAEQNLVGIAAGLALGGFVAVTANAACFLTTRAAEQVKNDICYSDTN
                     VKLVGLNAGVCYGPLASTHHAIDDISMMLGLGNIVILAPSDPLDAKQIFEYALRRKGP
                     VYIRMDNDKFPIIHKEEYRFQPGKADILQEGSDLSIFTMGSTAYEAYFAAQALQNDSI
                     NAEVINIASVRPLDRDSIAASIAKTGRVITVEEHSLHGGLGSLVSEVIAKCGLKAKLI
                     TRGIPEGCFSKAGPRNQIRAYYKIDKDGIIETAKQLLAAD"
     gene            complement(28746..29570)
                     /locus_tag="PLE88_01520"
     CDS             complement(28746..29570)
                     /locus_tag="PLE88_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOM75176.1"
                     /translation="MNPFNLSADKIAEKAKEIRKKIIQTNVHAGQGHTGSDLSETDIL
                     ACLFFRILNIDTEHMDDPNRDRFILSKGHGVCGLYCTLAEAGFIPEEKLDTYLQFDSD
                     LPGHPVRQKCSFVELNTGALGHGLSVGAGLALSAKMNHRSFRVFVLTGDGELQEGSNW
                     EAAMCAAHFHLDNLVMIVDRNKLQLADGTETIMALEPLADKFRAFGFDVHLTNGNSPA
                     AIIQTIEGLNMDNQKPKAVIAQTTKGKGVSFIENQPAWHHRIPNREETIRAIEELQ"
     gene            complement(29533..31005)
                     /locus_tag="PLE88_01525"
     CDS             complement(29533..31005)
                     /locus_tag="PLE88_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75177.1"
                     /translation="MNIMQKKITFGVIIGTRNIFNAHLAAEQRQKILNQLDRLGFGYV
                     IPDEKATRAGAIETRADSKICAELFKAHRDLIDGVLIILPNFGDELGIIQTLQLAKLD
                     LPVLVQACNDEISKVDVKSRRDAYCGKISVCNNLYQYKIPFTDTTEHTCDIDSELFAQ
                     DLKYFGFVCRVVKGLKNARIGAFGTRPAAFQTVRYSEKLLQAAGITVVPIDLSEIFAQ
                     AQALDSSAAAVKNKLAAIKDYGRIPKEIAPEAIVKQAKLSVVLDRLMEENELDASAIQ
                     CWNSVENNYGCAACLSMSMMGQCYMPSACEMDVTGVISMYTLLLAGGNIPALLDWNNN
                     YGNQRDMCVCTHCSNYPKDFIGSEIEISNLDVLGTVLDRKNCFGAVKGKAVPGPMTFF
                     RLSTDDTKGRIKSYLGEGVFTNDPFGMDGGIAVCKVNNLRQLLRYICQNGYEHHVGMA
                     RGLSARVIYEAVSNYMKWDIYYHNGQKYNESIQFICGQNC"
     gene            complement(31002..31178)
                     /locus_tag="PLE88_01530"
     CDS             complement(31002..31178)
                     /locus_tag="PLE88_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75178.1"
                     /translation="MFQKHIRFAAAVYALHKCADTAAANRMYGTYNIFSVLIHRVWRY
                     NISMGDLTELKEVV"
     gene            31831..32703
                     /locus_tag="PLE88_01535"
     CDS             31831..32703
                     /locus_tag="PLE88_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM75179.1"
                     /translation="MVHFDPKRKEFTPYGFTCERWEPVLMYRPDKHNEIELNYCDQGQ
                     LTYLFWGERVTVEGGRPAVFWAATPHQIVDYEDIDSYFVITLPLPWVLSWDLPSPFIT
                     QLLNGQLLQENQQLSAGYPDRQIFEQWYQDMTHHSEHCHEIVLLEIKARLLRLAANLI
                     ASNAFASGTSNSSSKPVGTQSVSKIECIAKFIAQNYTKNICTADIAKEVKLHPDYAAD
                     LFRKTFGVTLNHFITQYRVQHAQRMLVTTNNKILDIAYESGFNSLSRFNASFKQLCGC
                     TPREYRAAHQLHSS"
     gene            32871..34430
                     /locus_tag="PLE88_01540"
     CDS             32871..34430
                     /locus_tag="PLE88_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75180.1"
                     /translation="MKKSAVLRWLDAVLVAAFLFIGLVQAQEENQSAAVQEPKPGMVP
                     PLPPLLPAEIPAFPGAWGGGMFATGGRGGKVILVTNLDDSGPGSLRAAIETEGPRIVV
                     FRVAGIIQLQSNLTITHPHITIAGQSAPGDGICIAHGSLDINTYNVILRHLRVRRGIT
                     SGGQGSDNIGGHPHHHVIVDHCSTSWGRDENLSLYRAMMEYPDPQNPAATISVKTPAR
                     NLTIQYCISSEGMKPGHEFGGTWGGHDATFHHNLFACNTGRNPSIGMSGEFDFRNNVI
                     FNWRHRTIDGGDETSLINIINNYFKPGPATNDNMLSIIARIEQRDMYSPGNRFKNCSW
                     YPPAGKRPGKWYVAGNIVEGYPEVTADNWKGMLLLEGVGTLDMARVNSPFEGWPVNQQ
                     TALQAFRDVLAKSGATLPRRDAVDKRVVEMVRTGKVTAGNGIISDPNQVGGFPEYTFS
                     PDQVPSDRDLDGMPDDWEEKYNLDPSSASDSSQDADQDGYTNIEEYLNGTNPRQKVDY
                     TNFDNNVDIIS"
     gene            34515..36725
                     /locus_tag="PLE88_01545"
     CDS             34515..36725
                     /locus_tag="PLE88_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75181.1"
                     /translation="MKHHLSAVILVLTAVLTVSSVANPLRVDINCDSRQDMRTEGWEN
                     WCPAGGDMKRSFGNLTVSLRAPSGSIELSGNKSLVVNGITVGADGAVAAGVKPAVMEL
                     QIEGLAAGPHTFVGYHHAFQNAAGIYSVSIGDRKAGGIKTTSGQLQHNDETAASFIEF
                     EARSGEPVVVRITALDGDCVILNGFALDTSDPNKKALKPQPANYERHANGDDGKVTLS
                     WTPAQSAVSHNIYMAWDLQADAAVSKLDTAAKPLASVSGSSYSASIVPHNSLLHYAWR
                     VDSIDNKGNITRGDIWLFRVRHRAFPTAEGYGRFAIGGRGGRVLHVTNLNDSGPGSLR
                     EAVESTGPRTVVFDVSGLISLNSKLIFRSENEYLTIAGQTAPGKGICLRNYTFGGMGG
                     RDTVVRFIRLRLGDLTGQTMDGMGLAGCNDCIVDHCSISWSIDEAFSSRGAGNITLQR
                     SLISEALNIAGHSHYSSGTGHGYAGSIGGNIGSFHHNLLAHNAGRNWSLAGGVDQANR
                     HAGRLDIRNMVVYNWNHRTTDGGAKEVQFVNNYYKPGPASQILTYLNPQFENPAFGPQ
                     QYYVEGNMMEGIAGPEGPEGPFKGMHIRGRQDAPVTVSKPFFESYVRTHSAKEAYENV
                     LADVGCNVPVLDDHDKRVIQEVREGTAIYKGSKSQLPGLPDSQEDVGGWEDYPEIHRP
                     ADWDTDGDGMPNKWEIRQHLNPHDPTDGTIDPDSDGYTNLEEYLNWLAAGHTLD"
     gene            complement(36722..38026)
                     /locus_tag="PLE88_01550"
     CDS             complement(36722..38026)
                     /locus_tag="PLE88_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOM75182.1"
                     /translation="MEAVQKAALGFFFTALLVGLIAWAGSNEAIAKGAGTIAAAALPV
                     GLGAVPILRGYRFTAWIAAAVICPLLVPHVFLNPQITETFSININHPWAVLMIIQLVM
                     FGMGTQMSIRDFAGVVKMPYPVAVGLVCQFTIMPLVGFLLAKVFGFPPEIGAGLVLIG
                     SCSSGLASNVMAYMACINLPLSITLTACATALAPFITPFWLTTLNTGIEKNFEFVDAM
                     MSIFKIVIAPIGAAMVHDLLKHAGHTRKRVVYGIAAAGGLWLLFLAGGGWKVITKTFP
                     VPVLTNCVTVVSFLLAAVLAGVIYHFIALKIRRLDSFMPRLSMFGIIYFTAIATASGR
                     NELLTVGLLLFAAAAAHNLIGYLLGYWLSRLLALEKSDARTVAIEVGMQNGGMAFGIA
                     SDLGKLATLGLAAAIFSPWMNVSGSILANFWRKHPIKDNRTH"
     gene            complement(38026..38679)
                     /locus_tag="PLE88_01555"
     CDS             complement(38026..38679)
                     /locus_tag="PLE88_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOM75183.1"
                     /translation="MKKKRLTLIHTSGMMVPVFNELCRELLSDVEAVHMVDESLLKDI
                     LKDGCLSKSTARRAVNLILSADQAGTDAILVTCSSLGRAAEVGRELAESLVMRVDEPM
                     ASKAIEIGKRIGVIATLPSTLNPTVELLQRQGKGNIEIIARLCRGAFEAVVSGDAARH
                     DKIVAESILALTDQIDVIVLAQASMARVVSTLPAAKVTVPVLASPRLAIEHLARELA"
     gene            complement(38676..39632)
                     /locus_tag="PLE88_01560"
     CDS             complement(38676..39632)
                     /locus_tag="PLE88_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOM75184.1"
                     /translation="MMRMGNTRIAFIETMAELAQQDPKVVLVCADSVLVVKGKSFAQK
                     YPNRFIDVGIAEQNAVACASGMAACGLIPFVATYAGFITMRACEQVRTFVAYPGLNVK
                     LIGANGGIGAGEREGVTHQFFEDLGIMRTIPGFTVVVPADTEQVKHATRAIYQIKGPA
                     YMRIGSGRDPIVDCENVPFELGKIRILADYGRDIILFACGFVINRVLLAAETLSREGI
                     KAAVVDVPTLKPLDHSGIVPLLSQAAAAVTVEDHNIIGGLGSAIAELSVSACPVHLCR
                     LGVPDIFTRSGHPEPLLDYYHMGVKDIVDAAKTAIKRRDAKA"
     gene            complement(39629..40480)
                     /locus_tag="PLE88_01565"
     CDS             complement(39629..40480)
                     /locus_tag="PLE88_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOM75185.1"
                     /translation="MIKKLNSIRNMPIKALEDIARQIRIDIITMIYKAGDGHPGASLS
                     VTDIITALYFDVMNVDPAHPEKPDRDRFILSKGHGCPALYAALAGKGYFSRDELPGLR
                     SLESMLQGHPDMNKTPGIDSTSGSLGNGVGVGLGMVLASRITGQDYFTYVVTGDGEIQ
                     EGVVWEAAMAACRYKAGRLIVFVDNNGRQSGGLVDEISGLYPILPKWESFGWHCQEID
                     GHDFSQILDAVEKAQACTDRPSVILAHTIKGKGIPFMEDDNSWHKRVPTADEYREAMR
                     ILGGPYL"
     gene            complement(40543..41997)
                     /locus_tag="PLE88_01570"
     CDS             complement(40543..41997)
                     /locus_tag="PLE88_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM75186.1"
                     /translation="MEQKSQSVTPFKDIVNYSIGEGATSISMNGVNNFAMLYYAKVLG
                     MDAKYAGMALGLTLLWDAVSDPIMGHLTDNTRSRFGRRHPYMFFGGLATAAAFFFLWF
                     VPDFFKQPAFLFGYLVVINLIFRTAMTVFLVPYTALGFEICTEYEGRSRLQSVRYFVN
                     QAVNFFFGAMAWILFFPDRTNQDGSRLDGTHIASNYFNMSIILTITTAVLILYCVYFT
                     RRYAADTRCIKTHNSFKSFLSDFAQTISDKYALYVFLFIGIAQLGMMVVAQVQMFTYV
                     DFMKFTAFQKTVVHGAGMIAFGLGALLQAQLVRRYDKKQAGYAGVFFCIFGNAMLWII
                     FLGGLLPPETYWAIGKLRLPVSVIMFGIFQGMWWGGCGMLIPLSTSMIADISEIHQLK
                     TGILKDGSYSAVFSFVLKFAMAVGMYVNGCLMNWVGYVSGVENQTPDVIRKLAMLTFT
                     VGPIVMAAALPILILYPVNRTFMTQIKEKMEVHS"
     gene            complement(42025..44034)
                     /locus_tag="PLE88_01575"
     CDS             complement(42025..44034)
                     /locus_tag="PLE88_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM75187.1"
                     /translation="MNKLWFIHTLCAAVYLNVYAIGAADKIRPHVKLTDTGIEDVKWT
                     EGFWADRFRVCHEVMIPNMWDLLKDAGISHAYANFLVAAGLQEGRHRGPKWHDGDFYK
                     WLEAAAFVYAVTKDEKLNRLMDEIIDVIGKAQRKDGYIHTPVIIAEKQSEQNTEFQNR
                     MDFETYNMGHLMTCAAMHYKATGKKTLLEIACRSADYLCTVYQTRPENLANNAICPSH
                     YMGLCDLYRVTGQLKYLELAQNLIDIRWMVKEGSDDNQDRIPFYQQREAVGHAVRANY
                     LYAGAADIFAETGNDSLWKPLESIWRDIVTRKMYVNGATGALYDGASPDGSKDHHSIQ
                     LVHQAYGRAYQLPNLTAYNETCATIGFVLFNKRMLDITADARYADILELAFYNGILST
                     ISLDGKEFFYTNPLRVVQDEPFEMRWSRQRQPYISCFCCPPNTVRMIAESARYAYSIS
                     EAGLYVHLYGSNTLDTQLADGSPIKLRQQTNYPWEETIQITMEKAPAYQTALFLRIPG
                     WAKEARVEINGKPAGEMPKPESYFQIQRRWAAGDQVRLVLPMPVQFIEANPFVEEARN
                     QTAVKRGPIVYCLESADLMDNVKPMDIAIGRKQKLTSRFDPSLLGGVVVLEGIAMQRI
                     GQPWQDVLYREISEQDPRPLNIRLIPYYAWGNRGKGEMTVWMPIY"
     gene            complement(44027..47299)
                     /locus_tag="PLE88_01580"
     CDS             complement(44027..47299)
                     /locus_tag="PLE88_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM75188.1"
                     /translation="MQTAVKSVAVPDWENQQVNQRNRLPAHAAFVPYPDEAAARNCTP
                     ELSPYYLSLNGLWKFHWSPCPQKRPLYFYRPDYNVRSWDAIAVPSNWQMHGYDIPIYT
                     SSQYPFKVDPPRVTGTPEKGWTTYRNRNPVGSYRRTFSLPPSWSGRRVFLHFAGVSSA
                     FYVWINGIRIGYSEGSMTPAEFEITPYLRYGENIIAAEVYRFSDGSYLEDQDMWRLSG
                     IYRDVYLYCTAAVRIADFAVRTELDSDYINGRILIQPKLQGSGDQDMAGWTVQAQLYD
                     PSGNPVWDSPLSRNAYEILNPGFEAAILEQRTPQRGPAEFGWLSGAVKNPEKWTAESP
                     RLYTLVLTLHTDRQEVIEAVSCRVGFRRIQIRNGQLLINGRPVRLYGVNRHEHDPDTG
                     RTLSLERMVQDITLMKQFNINAVRTSHYPNDPRWYDLCDQYGLYVMDEANIESHGLFG
                     YLANDPTWSDAFLDRGIRMVQRDKNHPSVIFWSLGNEAGYGPNFAALAAWIRQADPTR
                     PIHYEGAQADAQDKNDPRDPDTVDVISRMYPRVHELYDSLRDKRWPKILQIAQDPRDD
                     RPVLMCEYAHAMGNAVGNLREYWDEIYGHPRMIGGFIWDWVDQGLRKKTAVGQDYFAY
                     GGDFGDQPNSKDFCLNGLVFSDRTLTPKVWEVKKVYQPVRIESVDNSIGRLRLTNLYS
                     FTNLNTLQPRWNLMCDGIVQQSGILQSIDLAPGQQTEITLPVPAADYSDPKGEIWLRL
                     SFHLKEKTLWAPAGHEVAFEQIALAERKPPVQIDIATLPSITVIEKEDGVWIQGEAFR
                     AVFGRSEGTLISLVYDNREILSQDAESPPGPVLQVWRAPTSNDKAFGKGRAYDWKQAG
                     LNQLTCKAEHFQISHRGPTHIQVRTTSAVAAPDHNGIRLQTTWTVFGNGVIDMDSRFE
                     PFGQLPPLPRLGVVMHIAHDYTIFRWYGRGPHENYPDRCYGAEVGIWSGTVEEQYVPY
                     PRPQETGTKTDIRWLSLTDAQGHGLLVVADQPIAASALYYTAGDLERAEHAYELTPHR
                     QVILSLDARHSGLGNGSCGPGVLPRYEVLPEPIEMKLSIRPCRAGTKQEIAESARRLY
                     YE"
     gene            complement(47378..50122)
                     /locus_tag="PLE88_01585"
     CDS             complement(47378..50122)
                     /locus_tag="PLE88_01585"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOM75189.1"
                     /translation="MSTTIRVAKLRCEYLINPLGIDEKKPRLSWIVESNRRGTYQSAW
                     HVRVASCTELLASGKADLWDSGRTEDDRSIHIVYEGVPLTSRQTCYWQVQIWDDRGIS
                     AVSDAAVWTMGLLEEGDWKADWIAAGPDYLNSAGYAIKPTLTEPGTPPWFRKVFAVRN
                     SIRKATLYASARGLAELYLNGRRIGNDIFVPEWTDYDKRIHYRTYDVTEMIQKGDNVL
                     GAVLGDGWYSGYVGWQETRGRYGLETSLLIQLEIMLADGATQIITTDNSWKCAAGPIL
                     SSDFMMGEIYDARYDLVGWDKPDYDDTAWSEVRNVPPPNVPLVAQRSEPVRMIETIKP
                     VSVHKSAPGTWIYDLGQNITGWVRLTIDGPAGMRITLRHGERLNPDGTLYTENLRRAK
                     ATDVYILSGRGKEVWQPHFTFHGFQYIELSGLDAQPPDGTVIGCVIHSALSPAGRFEC
                     SHAGINRLWLNILWSQKDNFLSVPTDCPQRDERLGWTGDAQVFSRTASYNMDVAAFFT
                     KWMIDVEDAQTPEGIFPDTAPRLREGANFVGLDGLGGAAGWADAGIIIPWLLWRVYDD
                     TRIIERHYDAMAAWLDYIRRTNPHGIRSKELGNNYGDWLCIPSDTTFRAQSPMKTLLA
                     TAYWADDAAKLSNMAKAIGRQLDAERFQEMFEYVREAFQKEFLLEDGRLTVDTQTAYL
                     LALAFNLLPQQARERAAEHLVENIRNCNWHLSTGFIGVSHLNPVLTAIGRADVAWRLL
                     LNEDYPSWLYPVLHGATTIWERWNGWTETKGFFDPQMNSFNHYSLGSVGEWLFRCAAG
                     IELDSHLNGFKKVLLRPFPGGGLTYAAAEYHSIHGLIKSRWQKDGDIITYDVAIPANT
                     CGRVYLPSVPRTPILESGRPVEGIPEIKALGRDGQFAVFEVPSGAYQFKCRYPNTSDK
                     "
     gene            50944..51873
                     /locus_tag="PLE88_01590"
     CDS             50944..51873
                     /locus_tag="PLE88_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75190.1"
                     /translation="MWDDFNFSYNLPKTYRRKMRAFTLIELLVVIAIIALLLSIIIPS
                     LNLAKRKAAAAICLSNAKNLSAAWYMYQEDNDGRLVNPNPTKVKIDGSHDDPRFIGWV
                     VDPIRPNGTMCNGGEGPNNPVTDDDEIRGIEKGALFDYLKSPDVYHCPADNQRKSKID
                     GSKIFRTYSCPRAIKRVTKFMNIKAPGMRFNFVEEAEGRNWNMGTWDFYNMAENGFWE
                     WRDPIGFNHGDSGILGFCDGHAEVHKWQVSYTLDRVRFFFSNPSTLNYGSPGLNDDYP
                     RLMAGAPPAESDPDIAYVATGWADQSRPMSGGY"
     gene            52424..55276
                     /locus_tag="PLE88_01595"
     CDS             52424..55276
                     /locus_tag="PLE88_01595"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOM75191.1"
                     /translation="MRYQKTAQAYLYIINYLLQPAFCAAIICCSGIGCSRDVFSGIQK
                     NHAVTIGSLRCEYMINPLGIDVKVPRLSWAIQSAENGVRQTGYQILVASEKSFLQKDI
                     GDIWDSGKVYSAQSVHVEYNGQELRSCQHCFWKVRIWDQNGKVSDWSPMACWSMGIMQ
                     PDEWKANWIQQRNPAAENASKNDLVNSIYPLPIFRKDFSVKPNIKNAIVHISGLGHYE
                     LFLNGQKIGNHFLDPAWSVYEKTVYYNTFDITSILTSGDNTFGVMLGKGFYTTKGDRR
                     IHFVNVYRPLKLIVQAHIEYQDGSNQIIVSDDSWKYTPGPLTHCAILGGSSYDARRLP
                     DGWNQPGFDDSTWQPAQQTSGPGGQLKAFIAPPMQTMQVLRPISVDEPETGYFVYDFG
                     QNVSSIPRLMVEGKAGQTIRLTPAEQRHGQTGTANNGKGRVNQARIGSPNYWEYTLKG
                     GGPESWSPQFTYTGYQYIEVAGAVPAGFLNPDDLPVIKELVSVQVRNASAPAGTFECS
                     NALFNNICQLIDWAVQSNMAHVLTDCPHREKLGWLEQSYLMGSAIFWNYDIAAFYTKK
                     IRDIRDSQDANGKIYTVAPNYPVFEDGPFRYSPEWGAAGVFLPWQIYQWYGDSRILEE
                     NYDMMKSYVDFMHSTSEDLIALPGLGDWYDYGHGQSLGPSRFTPKTLTSTATFYGCAD
                     IVSKTAKLLGNTKDAEKYTILCRDIKEKFNREFFDGRGTYQNNGSPQTANAMALVLGL
                     TPQGKEAEVLETIITDLKQRHYQQTAGDIGYHYLVQALSDNNRSDILYHITNRNEVGS
                     YGYIIKQGWTCLPEAWDATLTSSMNHCMLGHIQQWFQQCLAGIRPDFAGPGFKKCIIK
                     PEITGDIVWCKGTHDTLYGTIKSAWKLENGQFILDVTIPANTSATVYIPMKNDSKVAA
                     ESNSSRKGIHFSHIESDKAVFEIESGSYRFKVPYDS"
BASE COUNT        14623 a        14336 c        12479 g        13969 t
ORIGIN      
        1 catcaacttc cagcccagaa gctgagacag caaggctccg ccggcaaata atgtgccgcc
       61 aatccataaa actacagtgc ccaacaaggc atagaaagac atgaacgtat aacagccggg
      121 cccaaaacgc ttattcataa attccggcat tgtggcaatt ttcgtattca agtagaaagg
      181 aacaaagacc atacccagca aaaaaagaaa gatccaagcc atccactcaa aattagccgt
      241 caccatcccc gtggtatagc cggctccgca ggtggcaatt aaaaaagtcg gtccgatatt
      301 cgttccaaat atgcttaaac ccacattggc ccaccccatc gagcgaccgc caagaaacaa
      361 atcctcactg ttgcgctggc gatagctcac aaatgctccg attgacagca gaataaaaag
      421 gtagagcaca aacacccata catccaacgg agacaatttt accccgacag ccagaattgc
      481 cggcagctta atcatgggcg gaatcctttt ggagcgatgc gattgctacc tgaaccatgg
      541 gtgtccaaat atctttttcc cccggaaggg cctgctggca ggagtctgtc tgagcccacc
      601 atcgctggtt aacaggatcc tctctgttca tctgatcatc tttttcaaaa tcggatccca
      661 tatattccag atagaataat tcatacaatg tatccccaat ttccaccaga aaaatagaaa
      721 agttgcggtt gttcccccga accatttggt ctaccacacc cggccagacg gtctggtgca
      781 gactgcgata ataactttcc ttttcaggtt ttatcgttgt tatcatagag agaatttgag
      841 ttggcctgcc ctctctatcc agaccacgaa tataattaat ccattccatt tgcagccagc
      901 tgcagccgta ccggactgcc cgcggatggg gattgatcag agttttcagc tcgggtgtga
      961 ttgcttcaaa tgattgagcc gccttcaaat actgtttgcc tcctgaatac tgaaaataaa
     1021 ccacatgcca tgtttcagac tgaatctgtc ttttaaatac tgccagattg gatattccag
     1081 cctgcttaat tttttcgctt tcctgatcat tgcataactc taaaagcttt tgagacagtt
     1141 tgtcatcctg accagatttg gcacgtgcga tcaggcctac atgcgttggt cttcccgaca
     1201 ataaataatt ttcataaggc gaaaatgcat aggtcctaaa agaaaataac aagggaaaca
     1261 gcactaacca ggatagcttt ttcatacagc acctccaatc ttcaataatg gttaacaaat
     1321 caaaggaagc tgcactcctt tgggatcaat ctgatacaat ctgccgtata tttttatttt
     1381 aacggggaca ccttcaatca agttaaacac cgctttcgtt ttatccacag aaaccgcaat
     1441 tactccgcct cgataattga cattaaactg gtagcccttc cagtttttag caatccgagg
     1501 ccaaaatgac aaaacagatc cgtcgctgcg cattccgccg taaccgcaaa ccagatttgc
     1561 ccagaccccc gatgccgatg tagcatgaag gccctgctct gtattgcggt tatagttatc
     1621 cagatccagc cgagctgcat acttaaaaaa atcataagct tggtcagacc ggcctacttc
     1681 cagcgctaaa attgaatgca gggatgggga taaggaagat tcatgaattg ttcgagattc
     1741 ataaaattca taattggctt ttttttcttc caacgtaaaa tcttcgctga agaaaaacat
     1801 atggttcagc acatccggct gcttgatcat atttttccga aaaatgcgga tataggccca
     1861 gtttttgtaa ataggaatat ctgttattgg aatatcggca atctccacat gcggcaaatc
     1921 aaagtagccg gcatgctgtt caaatatttt tgtatcagga tcctgacgga tacgcatttt
     1981 ttgggccata tctgtccatg cctttggctc cgcaggatct agtgcgactt tttggacagc
     2041 ggccttatat tgttccgggg catttgcctt catctcctcc agaacatcca gcgtataaag
     2101 aaaaatcttc ttggccaggt aattcgtata aaagttatga ttgaccatca tatgaaactc
     2161 atccggcccc atcactccat aaagtccaaa atccccattc gaaggactgt aatctccggc
     2221 acttgcaaag aatcgggaaa tctcaagaag catctcgatc ccttcccgat ataaaaagtc
     2281 cttatcctgt gtaatcttgt catactgcca gatcgcataa ccaaccgcgc cgctgatatg
     2341 gtgttccaga tccacatgct gccaggtccc ggagtcctca tatccggaaa ccgtcgcaaa
     2401 aggatatcgt gcccccctgc aatccagatc ccgggcacgc cggcgtgcat gatccagcgt
     2461 atgatagcga tacatcaaga gatttttagc agcttgggga ttgacaaaca aatgaaatcg
     2521 atggcagtag atttctgtat cccagaatgc ccagccaaaa tacacttcgc ccgtcattcc
     2581 tttacaaagc ggatttaagt ggggattttc tccgtgatag gtttggtagc acgacaggat
     2641 ggaaaaacgc aggccctgca atacatcatc gtctccatca atagcaacgc cgaaacgatc
     2701 ccatacatgc tccatgaatt ggacatgctg tttataggcg gcttcaaacg tgacgtctgc
     2761 atacccagcg gctaatttgc atgcattttt ccagttttca tcggtatggt catttttctc
     2821 ccagtgattg aaaacgattt tatcaataca tgcggccttg tttttatcca aagagagtga
     2881 aaatccaaga ccgataaact tatcgtgttc cagcacgtgc ggagcaacgg gctgcgcagt
     2941 caacatccgg aagcttgaaa acaattgaaa gcctgtcgtc tttgtctgcg ccagaattcg
     3001 gcagcttgta tcgtccgaat cctttttgac ttcattccag aagtttttac ccctatctga
     3061 atcttttgaa ccggtcgtct gcgtctgggt ccagccggag gcaatttcat aacggctatt
     3121 aaagtcaagc ccaagcacaa tatccgcttt tccagagaaa tttatcggct ccaacaggat
     3181 gcgctggcat cctatttttg tcatatgcat actcaaaaag cgtataaacg ttatcttaac
     3241 ctgccggccg gaccgcaggt gccagacaaa ttggcgggta tacacaagct ttttcatatc
     3301 aaggattcgc ctgaaatcgc tgaaacagac agcagcaagg tcaagcaact ccccgtcaat
     3361 gcggatgcgc gtgtagagcc aatcaaccgc attaatcata gcgccgcttt tatcgaccat
     3421 tcccttaaaa agctggtcat ggccgatttc aagataatca tagaggtgat taaaatagct
     3481 gcccaacaga tgatcgccgc tgtagccctc ctcaaaatat cccctgacgc ccatatattc
     3541 gttcgaaact gaaaaaattg attcgctaag ccgctgtttt tctgcttgga atccttcttc
     3601 tacaacagtc caaggatctc ctccgtaata cttttctggc tgttttgtca agtgcatacg
     3661 ttatccgttt ctaaaaataa tacatcgaat actgtgagtg ttcaatataa atggctgtca
     3721 tattttgtaa ggttcaacct gccgcctctt ctccgatttg tgagtaataa tagccacgct
     3781 atgaattctc agaaatagca gtttattatt tcgtacagca aagattcttc ctgtgtttca
     3841 ggtataacat atcatattct tagaaccgca atgaaacaag atggtttggg gacaaataag
     3901 cccttcgcat gcagattgat taaattatgg ttgaaatcga gaaagaaatc actatattca
     3961 tgctataaca acgttggttc catagaattt ttgctccggt gcaggcaaga aaaaaagctt
     4021 taatccatta aggatgactt tgcatgaatc aagttatctg tattgtattg cttttttttg
     4081 tcattagccc cacaactgcg atgtccagcg aaagccgttc ccttgctgta cataccaaca
     4141 acaatacaga agaatcccaa acgtcacccc atcagctgca aagccctgcc ggcctaaccg
     4201 tagaagtccg gggccagacc tcgccccgcc tgacctatgc atcgggaaga cttcaacaac
     4261 accttcgcgc gtttggcccc gctcgcatta ttctcagcag ccataaccta aataaggtcg
     4321 aagaccgccg caatcctgag ggtttttatc tctatcgaga tgccgaaggc gtctttcata
     4381 tcgaaggtta tggcgactcc ggggcactct atggagcttt ggaattaatt gaccgggtta
     4441 aagaaacagg ccaatggccc gcgacgttag aggttaccga agcaccagac tttcagctgc
     4501 gaggaccctg tatcgggatg cagcttacat ccattttgcc gggtcgcggc acctacgaat
     4561 atccttacac acccgagaat tttcccttct tctatgacaa agaccagtgg attgagtttc
     4621 tggacttttt ggttgctaac cgcttgaaca ccctttacct gtggaacggg cacccttttg
     4681 cctctctggt taagctggag gactatcctt atgccttgga ggtctctgaa gaagtctatc
     4741 agcgcaacgt ggaaatttac cgcctgctaa ccgaagaagc agacaagcgc ggcatctgga
     4801 ttatccagat gttttacaat atctttgtat ccaagccctt cgctgaacac caccatattg
     4861 aaacccagca tgctgctccc aatgaactga ttgccgatta caaccgcaag tctatcagtc
     4921 aattcataaa gatgtacccg aatgtaggat tattagtctg tcttggtgag gcgctgaaag
     4981 gacaggaaaa tcaagaagca tggatgaatg aaacgatcat ccctgccgtt aaacagggct
     5041 tggctgcctt gggcaagact gaagagcccc cgattgtcgt gcgggctcat gcagtcagca
     5101 atgtaaaaca gatggtagaa tccgctttaa aacactacac aaatctttat accatggcta
     5161 aatacaatgg tgaatcactg accacttatg aaccccgcgg acaatggcag caggtgcatc
     5221 tggacatgag ccgtttaggc tccacccatg tagttaatgt ccatctgctt tccaacttgg
     5281 agcccttccg gtacggtgcc acagacttca tacgccgctg tgttctggcc tgccgtgacc
     5341 gtttgggtgc tcagggtgtg cacctttatc cattggctta ctgggactgg cctaacacac
     5401 ccgacaaagt caatccttat ctcaaacagt ataaacgaga ctggctctgg ttcgaagcat
     5461 gggctcgtta cctgtggcgt ttagatcgtg acccagtcca agatcaacag tattggattc
     5521 ggcggcttac cgagatgtac ggcaacccag aagccgctga gctgatcctc aaggcctaca
     5581 atgaatccgg atactgtgcc cccttgctgc tccgccgttt cggtattacc gaaggcaacc
     5641 gccagacttt ctcgctgggc atgtttctgg atcagctggt caatcccgga ccatacggtc
     5701 ccatcagcgg tctctgggaa tggcatgctc ctgccggcga acgtcttgaa gagtatgcta
     5761 aaaaggaatg ggccggtgaa tcccacagcg gtgaaacccc tgtcggagtc attgaggaag
     5821 cgaccggtca tgcgcaatca gctgtcaagg caattgatga ggcggcctca cggatcacac
     5881 gcaatcaaga tgaatttgat cggctttgca atgatataca ctgtattgac ttgataagca
     5941 agttctatgc cgagaaggtt cgggccgcca ttttggtact ggattacaac tacagccgca
     6001 attcagaaag catgcgcaaa gcccaaaaac atctggagca aagtctggag cattaccgta
     6061 ccctgaccga gcgtaccagt aaaacctatc gctatgcaaa caccctgcaa acgggtcatc
     6121 gccgcattcc gatccgaggg atcaaagacg gcttgcccta ttactatcat tgggaacaca
     6181 tgctgccgct ctatgaaaaa gaattggaag agtttaaaaa tcaactgtct caccttgatg
     6241 ttaccacacc gcagcctcgt cgggccggcc ctaaactcaa aagcgtaccg tttgaactga
     6301 ttagccaaga ttgtgaaact tatgaggtca aggtcggcgc caagcccttt acagaccgcg
     6361 attatcagat cctgcaaatc gcagaagaac tggaaggctt ggtgggtatt cgcttctcgc
     6421 ataccgccgg caaagccggc caactcaagc ccattcgtct gaaaacctca gtgcccgtac
     6481 aaatcctcat cggctacttt aatgaaaacc gtcccatttg ggcacaaccc cctaatcttg
     6541 atatcgacag tcaggccgct caatacggcg gcgttgaaag cacaatgcaa aatgcgctgc
     6601 aaattgaagg gtgcccaaat gtcaatattc atgtgttcag ctatcctgcg ggagaaattg
     6661 tgtttgatcc ccgaagtcca ggcagctact tgttcctggg agtcataccc gctgatatgg
     6721 aaataacatc acgcgatgct ggaatagcac cggggcgaat gtaaccgagc cgacactcta
     6781 agcgatgtgc attgtaggtt tatctacttg ctataaattt aggtcttact cttttcggcc
     6841 aggtcagggg gtatattttt ttgtctttca atccatccgt aaactgtcat ttgcctcacc
     6901 tccttatccg gcttgaaaaa gaaactggcc aaataaccca caacaaacaa aatcaggtgg
     6961 ctgtaaactc caagcatata tttatgatgg ggaaagttat accgtcccaa atcgagtata
     7021 agctgtttct ctgagccgcc cagatcgaat tttgtggagg tcagtacagc ataggccgta
     7081 aatacaatac aggctgttat tcctatattg agtccttttc gatttgcacg cactgtaaaa
     7141 aaagcaagca aaaagatccc ggcgatcccg ccggaaaaga tagcatacag ttcaaataat
     7201 gttcccagaa ttgcctcgcc tcctaagtgg atatacagac aggcgatgcc gacggagatg
     7261 attccgcacg tcaagacaat catctttccc aaatgcaaac accgctgatc atcagcattt
     7321 ttcttgaatc tcttgtagta atcttcaaca cctacggccg caagacaatt caaatctgaa
     7381 tcgagggtag acatagcggc cgaaaataaa gccgaaacaa tcagtccagt caatccgaca
     7441 ggcatctgcg tcataataaa atatggaaaa acctggtcgc ctgtaatacc ttgggccagc
     7501 agttccgggc gcctacggta gtaaacccac agcaatgtac cgataaaaat aaacaatgtc
     7561 catacaggca cacataaaaa tgtccccacc aaggccgctt tgattgcttt tttatcggtt
     7621 tgagcaacca gaaatcgctg aacaatggtc tgatcggtac cgtatttttg aattgcgtag
     7681 aaaatcccgt tgataaacat tacgaaaaat gtaagtcgtg taaaatccca tgtgaactcg
     7741 ccaatgctca tcttcccgct ttcccatgct gtctgaagca ctggaacagg gccttgtgct
     7801 gagtgaagca gcaagataag acaactggca atcccccctg caataagaat aagtccctgt
     7861 ataacatcca tccagataac gccttcaatg cctccggcta aagtatacag aattgtaaca
     7921 atgccaaccg ctgcaacgat accataagtg ttttgtccgg tcatagaagc cagcgccata
     7981 gcgagaagat agaaaacggt tcccattttt gtaaagtgca tcatgataaa tcccatagaa
     8041 ctgtacaagc gggcaaaaaa tccgaaccgt ttttcaaaat attcataggc actgaggcca
     8101 ataaaccgcc gatacagagg aacaataagc cagacaaatg tcaataatgt tatcggtaca
     8161 gccaatccct gcaccagcag aatccaattg ccgctgaatc cctggccggg ataggctaaa
     8221 aatgttacgc tgctgatgag ggtggccaaa actgacatgc cgattgccca cgctggaatg
     8281 gttcggcttc cgacaaagta actatctgtt gatttgccct tttcggaaaa ttttttgccc
     8341 agataaatta tcaatcccaa gtaaaatagt ataatcaacc aatctatcca atgcagtgtt
     8401 tcacggggca cagtaaaatc ctttcgaaaa gaaattcaaa atacctgccg gccaggcata
     8461 gctgccgggt ttcgccgtca gatagacatt atcggcgttc attaccttca ttcgtacaat
     8521 gcctggttcc agttgaaagc acggtgcaaa acggacccat ccgaagctgt ctgcgatcgc
     8581 agaggccgtt gcgcctcctt ccacccacaa ctcgcttaaa ggaatctcac agatcactgc
     8641 ctgcaccagc ttggctgtta gaccgcgaag tctgacggca acatcaggga ctatttcacg
     8701 atcgattgct acgataacac ggctgctgta ttgaagactg ccgattacct cttcagtcca
     8761 ctgcagaaat gtgttctgtt cgtcacaatc cggtctaaac agcccggccg gcatcctgca
     8821 tactttcatc cctagacttt ctgcttttcg caaagcctga cggctggcat tcgaactgct
     8881 cccgcagaca tacaatgtct tggtattcag ctgatagtct atttcatcca catgcggttt
     8941 tttggaaaaa cctttgtttt tcagaatcgc ttcaaaatac tccccggctc ctgccggcag
     9001 tgtttgctca tctattcgct gtgcccaccg catcaggtca tcggtcgttt cagcctgtcc
     9061 aattatgatt ccttcattgg gaaacggctg atgcgtatca agacaacata cttgatcgaa
     9121 accgaacaga ccctgcattt taagaacatt tgaagtagct gcgggatatt caggatcgcg
     9181 tgcaaattct gtctcgctca acagctgatt cctgataaga taaattccgt cacgcacaat
     9241 cctgcctttt gagggattgg ccggaacaag caacactctc gccgcgtgca tttcttcacg
     9301 cagcgtttcc aattcacagc caaccggccc acgaagaacc gaatcggtct ttttataaat
     9361 ccactctata ggaaggcctg atgcattaag accctgagct aggtgccgta ttttctgccc
     9421 agcctgctga ggtgacagcg atcgggtgtc tgtgtcgata accaacagct gtgcgtcagt
     9481 cttcgaacaa aatcctctct ggagttctgc ggtcatacca caaccacaac cgattcctgc
     9541 aatctcggcg gcacctgtca agtcatctgc tacaacaacg atcataatat cccttaaaga
     9601 gaatcgtatt acaccgccag cttggcagca tattcgattg cgagaatcaa gctttcctcg
     9661 tttgcagtcc ccttgcctgc ctggtcaaaa gccgttccat gatccaccga cgtccgaata
     9721 atgggaagcc caagtgtaat gtttacacct ttaaccgact cccacttttt cgatttatga
     9781 ttcagtacaa atccggttac tttcacagga atatgtcctt ggtcatgata catcgccaca
     9841 gcaatatcat actggcctcc tttgacctta gaaaacagcg tatctgccgg aacaggacca
     9901 tcggcatcaa ttccaaaaca tctggccgtt tctatcgcgg gcattatttc ctttgcctcc
     9961 tcatccccaa acaaaccgcc ctcacctgca tgcggattca atcctgccac tccaactgca
    10021 ggctgctcga tacccaatcg cttgcataga tcgtgtgcca atttaattac ttccagaatc
    10081 cgctccgtct tgacaagttc acatgcccgt ttcagcgaaa catgcgtaga tacatgcacg
    10141 acccgcatat ttccctcaat cagacacatg gcatactgcc gtgtctttgt gtatgcagca
    10201 aatatttcgg tatgacccga ataagggtat ccggctttat tcaaagactc cttgttaatg
    10261 ggtccggtga cggtcgcatc gatttgaccg ctcatcgcca gcattattgc cttttcgaca
    10321 gcctcaaagg ctgccttgcc ggccattgga cagacttttc catactccag cctgcctgta
    10381 tccacattgt tcaaatccaa gacatccgcc gttccatatt ggaatagcgc ttccccaatg
    10441 ttcgaaaccg ctcttacctg cagactgact cccgcaatct gggtcgcccg ggcaataaca
    10501 gatgcatccc caacaacaat cggtttggct attgtataca cgtttttatc tgcaagcgct
    10561 ttgaccgcta tttcaggacc aatgccggca ggatccccca tcgttattcc aagaatcggt
    10621 cttcgttcag tcatactcca tctttcgcgg aattatgaaa taactgtatt aatgcacaat
    10681 tttgccattt gctgctgtat ctcggctatc tcatgctttt gaactgtctg aagaggaggc
    10741 aatactgtgg gttcgcacag cccaagaaga tgcatagccg ccttcaatgc ggccaacgac
    10801 tgccccaagg atctggggcc ctgatacagt gctgaaatct gatccgtctg cttttgaagt
    10861 tcctgtgcag cggatacgct gccggtgagt gcattttcat aaagcttttt atagagtccg
    10921 ggctgaagat ttgcagcact tggaacaatt cctgccgccc ccattgataa ggccttagcc
    10981 gaaagcgctg cacagccgac aacataagaa aaatcgcttc ggtgcgcaaa tcgttcaaca
    11041 gctgcttcca atcgtccggg gctgttctcc gaatctttta agccgactat ccatttatga
    11101 cggctcagtt tttccacaat gtcaatagga agtgaaacac cggtcgtctg gggtatatta
    11161 taaatcatca aaggagttgc aatggaatca gccaatttta taaaataatt gaacagctct
    11221 ccggcattca aggaataata ataaggcgga tgagccacca tgatatcagc cccaaagtct
    11281 ttgaagtccc gggccatcgt gatcgactcg ttaaaacaat tgctcgatat gccggcataa
    11341 acaagggccc tgttatcgac gcaatcggcg gttatttgaa ccatcttcac tttttccgcc
    11401 agcgatatcg aagcagcctc gccagttgtt cccaggacaa atatgccgtc gacgcccccg
    11461 ccaatcagat gctcgacaat ccgatgaaca gcctttgcgt caatgtgtcc ttccaaggta
    11521 aagggggttg ccatgggaac aataaccccc tgacatttta ctccattcga catattggca
    11581 tgcatttttt caaattgcta tctaacacaa ttcaaacagg cagatttctt tattcgcaag
    11641 taaaaaaatg gagagctcct ccgcaggagc cctccatcac gctgttagcg aattcacaca
    11701 atacggttat tcggcataaa ttcggtaatc atccagccag cggacggcta tcttggcaaa
    11761 atcagaaaga tcaaccacac agttccgatc gtagtcatag tcgtacaaat catagttttc
    11821 catatcacaa acggatgcat cacccgttat accgtagtat tcctgagcaa tttcctcttt
    11881 tgttcgggca tagttataga tcttgaattc atcgatcctg ccattaaaga gaggggccgt
    11941 aaatccgata tttttgccga tataatgaag tgtctttgtt atctctgtca tcttgggtat
    12001 attcattatt tgtggggttt cggtctctgt tccgtgttct ccgccaaaat aaatgcgtcc
    12061 tgtgccgcct tggccgtcct catccgcttc gatggttatc gccacaaaat accaccttcc
    12121 ggcggcaata tccgggtgtt ccacgtcgag ttccggatta tgtgccactc cttgagcatc
    12181 ataatatctc agctcacaac gagccacatc cagaccgttt tgtctgggat catcttgccg
    12241 gtaaagcaat gtaaagaaaa tatattccgt cgaatctttg ccaaagtcaa agactcgggc
    12301 ccatcggtcc ccatcggttg tattaaccag agttgtcgca taacaccaga agctgatggt
    12361 aatatcatca taatgagcca ctgtgttatc tgcaatctgc gcgtactgat tcttcggatt
    12421 gtcaagccac aggtttttac ccacaagcga atacgcatcg ctcaagctcg ctccattctt
    12481 caactccata tctttgccgc ttacgatatc cggggtgtaa atcttgccgc ccgcattata
    12541 ggtggtctca aatgggtagt acgtcatcaa ttgcttgatc tttaactcgc cgctggtcaa
    12601 agtctgagcc gtcagtcctg tcgccgtttc tgtcccgacg caatagtaca atcccgcatc
    12661 ggctaactga gcgtcgcgaa cagtcagtgt atccgtgttg acgccggcat attttgcacc
    12721 attggtcagt gcaccaacac cggctttgta ccattggaag gagtctgtat tgttgtagta
    12781 ctccactgtg aaaacagcat cttggccggg atgaacaccg agcttgtagg gatcaacgtc
    12841 accgaggtag gggcctttct gtctggttct aaaactccag gccggactct gatatttcag
    12901 agacagctcg atcccgttgg gttcataagc aaggactttc cagaaatagt ctgtgctgta
    12961 tgcaaggtct tgagacaatt cgctctcaag ggtaatggca tattgtcccg gcgaaacgga
    13021 caggccattc acaatcttat gcgcctctga cagattggga tccgggccga atagaacatc
    13081 tatttttata atctcgctgt cggcaacagt gaaaattaaa tcgttttggc ctgaagtacg
    13141 ttcaactgca acataattgg tgccattctt cggaagcgga ttgacaacgc tgacaagttc
    13201 agcaatctgg acattatcaa tcacagcaag gtcttcggtg tcgctattcc catcgccgtt
    13261 attcctaaaa cgcaaaaaaa gctcgccgac ggtcgaatcc aaataatgaa gcgtaaccga
    13321 atctgtgata acctgacccg gagcaaccgc aggatatgcg acacgcatag agtcaatcag
    13381 cttaacacct ttgactccgt caatatccga tgaacgcggc ccatcggaac ctgcaaatga
    13441 gccgtccgat tcatagattg acacagtaat aaccagatca cgcggctccg ccgcatttgt
    13501 cgttcgcccg atgtcatact gaatctgcag cgcaggcaat ctttccgtat tgaaaccgat
    13561 gctctgatag gcccaagagt tgccgtcagc taaacccata tactgcgtgc cgtttggact
    13621 gttaaggagt cgcaggtaag tccctgcggc cccagtgcct acattatagt cgaaccagta
    13681 gggcacatcc tcgcgaactc ctaaggcctc aaagtccccg ttggtaatcg acagagctga
    13741 tacgcccgat gccatgcata tacatattaa aatgtaaatg attcttctca tttttccatc
    13801 ctttcattta aatgtcttct taaagggtgt ctggttagga ttcgagattc ataaagtaat
    13861 tcatcagcca tttttcagca attaccaata aatctttaat atcgaccaga caatcaccat
    13921 tgaagtccca gtcccctaaa tcatagtctt ccctatcgca gataaagctg acatccgttc
    13981 gcacatccat atattcctgg gcaatctgtt cggccgttct ggcataatta tagatcttca
    14041 cttcatcgat caagccgtca aagttcggat aagaccctgt ctccggtgcc gcgatggctt
    14101 tgccgatgta gttaagcgtc tttggcagtt ctgagggact gtacatccct cctcctccac
    14161 tgtattgacc attaatatag agcttgccat cactgtcttc gatggttaag acgacataaa
    14221 tccactttgc gccgtaaccg atatcaccgg tgccattgag ttcattatca tcacccgcaa
    14281 cattcatcac gcagcgtgcg gcattggtgg cctgaagcaa ggtcaggtaa aaataattgt
    14341 tcgcatcctg accaaagtca aagattctgg cataccgttc ataatcaaga tcaaggatcg
    14401 tcggcttaac ccagcagctg atagtaatat cccgatgatg ggcaatgctg ttattgagga
    14461 actctccata ctgcgtatgt gtcactcctc ttggattaag gagtttcaaa tgtccgccgg
    14521 cgatggaatt gggatctgca ccacttttga cagatgcccc ccctctcaag tacaaatgct
    14581 taccgctgat gatgtctgga ctatacatgc cctgggcatc gcttccggtt gagtcaaacg
    14641 gcaagtacga ctttaattcc ttaatggcca attccccgcg attggtgctc tcaatggttg
    14701 cgccggttgc cgtttctgtc ccgatacaat agtaaattcc ttcatcttct gttcggcaat
    14761 taagaaccgt cagcttattt gtgctggtgc ccgcataatc agcaccatct gcgagggcca
    14821 catcgggatt tcctgcctta taccactgca aggtatctgc ttttaaggta aacggcaccg
    14881 tgaagacagc atcttcaccg ggccaaacag cctgaatagc cggcgtgatg ccgcccagca
    14941 agggaccata ctggatggtt gtgaaactgg taatcaggcc cgtgtatttg agggaaagcc
    15001 ctccgctgcc gtttgactca taagcaagga ctttccagta atatgttgta gtccagttaa
    15061 gatcctgagc caattcacct acaagatcaa cagtattcaa acccggtgtt accggcatac
    15121 ccgcaacaat cttcgttgca gtaatgaggt tgggatctgt accaaacaga atatcaactg
    15181 caacaatcgc aggatcagca acattgaaaa ctaaatcatt ctccggagag gcaagctcgg
    15241 ttgctacata aacagctcca ttttggggtg atttattgac cacataggcc ggcgataatg
    15301 tgatattatc aaaactcacc cacggtgtta cgttccgtct gaatgcattg atgcggagga
    15361 acaattcttt gcccacagta gcacttgtca aatcgatttg gaatcgttca tgcattacaa
    15421 caccttcagc cgcatatctg attacagaac cccggccgat ttccgtaata ggagcgttcg
    15481 gattcgcttc ggttttcctg aatccataga tgtcatgagc ggccccatcc cattcgttgg
    15541 gttcaaacgt gccgtcggac tctaaaatca taacggtaat ccccatctct cccggcgtat
    15601 aggtagggat caagccccaa tctaaagcaa tttcggccac cgacgggttg ccgtcataaa
    15661 tgccaatact ctgataaata taggcatgat ttccgccatc ctcgctggta ttagaacctt
    15721 gtccattgca tgcaattttg gtacgattca tgctgaagga attagcagga ttccaaaagg
    15781 ggccagctgt ggtagcaagc gtgggaccgc cataatcata ccaatatttg atatcataat
    15841 tcgaaccaag accagtctgg ttttcaaaat tgccatttgt aatcgacagc gcggaagcta
    15901 ttggaacgac gcttaagcat attataatac ataaaattct tctcattttt ccatcctttc
    15961 aatatattta ccgatttcaa agaccgcctt atgccgcttt ataattccta caggcaggca
    16021 caaacataaa tgttttataa ttgcatcata aaaagtataa tctcttcatt aatgataaag
    16081 atataactta ttatctagga atgacgatgt acaaatcaga tattcaacat gcaaattccc
    16141 atcaaagggt aatcccgcag acttcgtatg caagtctttt ttcagcattg agctggcgct
    16201 attcggatag cgccagctca atgcaccaca tacagtgaat tgtcagttgt tacaatctac
    16261 ttgcgcttgc gcagcaaggc cagaccaccg aaaccaagca gagccatcgt tgccggctca
    16321 ggaatgtgcg gttcgttcgt cagcatcggt gagaaaaggt tgctggcatt ggatttctcg
    16381 ctccacggac tggctgtatc cttgagcagt gttgtcattt ctttgggatt gaacaaataa
    16441 ttaaagttca gccagttggt gttgtcattg tcataatcgt gagcaattcc catcacaatg
    16501 gtcagcgtct tatggtcagt ctcctgtgcc atcaggatcg cttcatgcaa tagacttccc
    16561 gctggaagag taaagtcgaa tgccccaccg acaggcaggt gcgcgggggt gcccaccaaa
    16621 gcagcactat cgtacagttg ccgccccaga tagtataagc catctgtcgg ggcaatagga
    16681 cttccggttg ctttggttgc gtaatcatta tcaaggctgt aacccggtgc attgcttggt
    16741 gtgattgtaa gttcatccca attggcacca tcgatcgtgg gatccaaaat gaaataatcc
    16801 caaccggtgt ttggaccaat cggttcaact gtatcctgga ttcgactgat gaccaaattg
    16861 gtatttcgat aggtcaatcg tatggtgata tctccagcca gatcttgcgc cgtgatgcct
    16921 gccacaccaa acttcagata aatcacactg ttgcgccatt ttgttgggtc tgtactggca
    16981 tagatgcgtg aggcaatttc cgtacttgtc cccctgtttg ttgtcggagt ctcctctcgc
    17041 aattccgcat cagccccacc cgggtcggcc gtggtgacgc gaaacgcctg gctatccgcc
    17101 gccagcatca ctactgcagc aagccccact aacactaata atttcttcat ctttccaacc
    17161 tccaaacaaa aagttaacgt ttattatttc gactacatag ccgaaatcca catttcacga
    17221 tttatttcct gaaattatct taactttttg tatttcactc ccacagtttg gctttttttg
    17281 aaaaaagccg aaatggatac taaatgagca aatttcatgc tcttataaat acttatttta
    17341 taacaattca acgctaattt cttcccgatt ttttagaaaa aattaccata tatttagatt
    17401 cacagaatat ttcgtataaa aaaggggtat ttatgttaat tttgagggaa aaaccgcatc
    17461 gtaaacaata aaaaaacctt accggattaa ttttttataa aaaaaatcga aaatttgagg
    17521 gaatttattc attcccaacc tcgtagatct atgttttctg gttattatcc ttcatctttg
    17581 aaacagccgg cctcaataca tctcgtgtat atagatctaa taagtcttgt tattaacaac
    17641 ctttgccctt catgtcatgt tatagcatag gtttcaatgt catgctcttt ggggagtatt
    17701 atgctaaagg tagaatgctt gatatcgtag attcctttca aatccctgta gcgaaatttc
    17761 atagagtggc tattaagctg aatgaaattt gatgcatatt cttgcatctt ttcaagatat
    17821 atgaataata gtggacaagc ttgacaagac tgtcgtgtca atttttttta tcaattccgt
    17881 ttatggagga ttcgagcatg tcaagatgca taaatctaac cgcagtaaat gcaatcatgt
    17941 tggtctttat atcaactctg caagccgaaa ccgctatcga ggtggcggtc aagacacagg
    18001 ccgcaatcga tgccggcgct gcgcccgcct ggatgtcacc gaatctgcct gaggatatcc
    18061 aacaaaacgt tcaaaacaaa ggtaaagaac tggcacaatc gctaaagtta ggcaatgaaa
    18121 tcaagcagtc caaagtggca gatctattgg ccgaacatta tggccgggta tgggcatggc
    18181 atcagcaggc agacgaaaaa ctggatgctg cttggctagc ttgggatgat gcccgtgatc
    18241 cagccaaagg aggaaaaaat gagctgaaag ccctgaccat tatggtcgaa cagattgagc
    18301 ccatctatgc cgaatttgct ccacagattc agggactgct cacagctctg cgcaaggaaa
    18361 tcaccgatga acagattata gcgctgctgg atcgcattac gcgttctccg ggcgtcgacc
    18421 gcacctacaa tgcctatctt gaaatggtgc cggagatgaa agaagaggaa aaaaagattc
    18481 ttcgaaatcg tctggaacag gcccggatag aatcgttgtc tgcctggagc gataaatcca
    18541 tagtcaaaat cttcaaaaaa tacaaaatcc gcaacgaatt ttcgattgat gacttcggct
    18601 acgactatcg caaacgctac gaagcctggg tcacccgaac caaaagcaaa tgattcatct
    18661 gcataatgtt acgagatcgg tgtcttcatt cagccgaagc acctgtgtca aaataaggtc
    18721 cgttaatgcc gctttggctt ttgacgaaca attgccctca taatgctgac ttgaatttgt
    18781 tagtttgcat cttagcggtt atacagagac gattttactg ccagcactct gaagtaggca
    18841 gccgattgca ggtcagccaa tcttccgcaa gattttccag atcacctaaa tcataccccg
    18901 tattgtcccc ctcgcccatc caagcctcag caaggactgc gaaatcgctg atattaacct
    18961 ggcagttgcc gtcaaggtca gaagcgattg gagttgaaca ggaatagaca gtcagagtat
    19021 aggtatgccc ggcctccgtg ggaaattcat aaagatcctt gcctggtgaa tgcaccgcca
    19081 cacgatgagc tgcctcggat acaacaatcg gccaggctga acgaatacgg catgtcttgc
    19141 ccgccagcga tcggatggtt gcggtaagca gctggtgatt ttgccaggtg atatcaactt
    19201 caaagccgcc acgggctctc aagccggtta cgctgccgtt tgccattttg tccggcaggg
    19261 ccggcagaag atacacttcc ccctgatggc tctgcaggaa gaactccgcc gttcccatca
    19321 agacgcccaa attgccgtca atctggtttt cattgccctt gcgggaaaag gtcatattat
    19381 ctgtatggga tttaccgagg atcttgttga gaatcatata agcatgattg ccctgtagca
    19441 gacgcgtgcg gcagcacatc ttccaagcct gactccaagc cgtattattt gtgtcgtctc
    19501 ccttccaatc caagaccacc ttgacagcag ctgccaactc cggggtatga tggggagtaa
    19561 tgttgccggc cggcatcagg tcaaccaagt gggacagatg acgatgcgta ttggggacat
    19621 ctacatcctc cagccattcc tgcagttgac catatcgacc aatttggttg ggcggcagct
    19681 gtgctcgcat ctgctctaat tgctgtcgga atgccgcatc tgcatcgaga atcttgctgg
    19741 cttcaataac ataagtgaac aggcaccgta tcaattgaca gtccatggtt gggccggcca
    19801 caagttcgcc gttatcaccc aaggcaggat tgggctgctc cggagaatgc gaaggattgg
    19861 ttaggagata gccggcactg ccggggcgcg gatcccgcac cagaaagccc gcaaaaaact
    19921 cggctgcccc cttcatcaaa ggataaacct gagccagaaa ggcagcatct ccgctatatt
    19981 cataatgcca ccatagatgc atagaaagcc aagcgccccc tgtcggccag aggccgccgg
    20041 cgttattaat gggcgctgcc ccgcgccata gatccgtatt gtgatggact acccatccat
    20101 cagctccgta atgcgcagcg gctaccgctg cgccggtctc ggacaaatct cgaatcatat
    20161 caaacagcgg ctcatggcac tctggaagat tagctacttc tgcagcccag tagttcattt
    20221 cctcattgat attcagggtc atcttgcctt cccatgaggg ttcgaactca ttgttccatt
    20281 tgccttgcaa gttcagcggc tgcgagccgg gacgagaacc cgaaatcatc agatatcgag
    20341 ccatttggaa attcaaggct attaactgga gatcgtcggc attgaacagc gaccaatcat
    20401 acttggcagc atccacccca gcctcaatcg catccagacg ctcattgatg ggaagattga
    20461 ctttattcga ggtgcctaaa tccaaagtta ctcggttaaa gagagattga taatcattca
    20521 gctgatcttg acgcaaggca ttaaagccct tggcggcggc cttggttacc gtttgctggc
    20581 aaatcagact cggattgccg ctgatatcat catagcgaac aaagtttgac gcggccccta
    20641 acaccaatgt tgcggcgtcg gcaccgctga cctgcagagt atttccaatc actgctatgg
    20701 aaccgccttc gcaaagaatc cgcacccgtg cttcgaattc gatattgctg gccaaaccgt
    20761 ttttgcccac atgatcgacc gcaccgcgca gcacaacatc actgccgctg gcgctattgc
    20821 ttacaactgt gtgcggtgtg gttaaagcgc aagtaaaatt gattttgccg aactggctgg
    20881 cagtcagacg caccactatt acattgtccg gatagctggc aaaacagtct ctctgataag
    20941 ttacaccgcc ggtcacataa cttactgaag cggtcgccgt gctcaaatct aattgacggc
    21001 gataatttgt tggggcggaa tggttaaagg tcaatcgcag ttcagccgtc ggctggtagg
    21061 gacactgacg cagaggaacg ctcataaaag aaccgctggc gtcggcgtag gcggccagac
    21121 cctcgccggc ccacacatgc tgccggatca gttccagcac attgacggct ccggcgtggg
    21181 cataattatg cggctgacca gcccagacag tatcttcatt gaactggata atttccgtgc
    21241 ctgtgccgcc gtacaccata gcagccagct tgccgttgcc gatgggcaag gcctcattgg
    21301 atgtcacatc ggatgtataa gaggccgggc tgttataaaa caagaccatc ggtccgggat
    21361 ccgcccccgc cctatataca agaagaggaa aaaacaccag tattatagac acaaacaaat
    21421 gattgcattg catcagacta atcctcccat tcttgccgct gaaccatctc ttgattatgc
    21481 accggacatt ttccttcata tcgctgataa tcttatgcgt tgacatactc ggatctaaac
    21541 ccttcccatc gctacagaat tttcagtttt gatgacgaaa cgataaagtt tacacgcatt
    21601 tattgcaaaa gactttgaat ttccgactcg ctcagcgctc ggtcatagat gcggacttca
    21661 tcaatctgtc cgccgaagaa tgattccagt gctccatccg gcttctgcat aactccaatc
    21721 cgcaccgaac aatcagttcc agtattaatc tccacagtcg gattggtgta agtccctgca
    21781 tccagctgtc catcaatata caggcgaagg ttctgtacgc tggccggtcc agatgccggt
    21841 tcctgcagca cagccgccac atggtgccac tgtccgtcat tcacactccg attgctgaag
    21901 atattgcccc cgccgacacc caattgcagt tttgaattag tgataaaaaa caaccagcgc
    21961 gtccccggat taagaccgcc gtagcttaaa atcatttcgg aaatctgcga ggtttgtatc
    22021 cacgcacaaa cggtccgcat atggccgcag ctgacacctt tgtagccggc tatctctaca
    22081 taatcatcta tcccgtcaaa tgccagtgct ccgccggaca cacccgtcac ccacgttggg
    22141 ccgttgacca agccggctgt tcgggcatag agagaaccat cggcgctgtt atccccgctc
    22201 atctcatcaa agggcagata caaaaccaaa tcttcatcgg ccagccagtt gtcagttagg
    22261 atttgcaaat cgggcagact cacattccta tccccgtcca agtcggctcc cccgcatgca
    22321 gggatatcct cacagccggc cagcagccat tcggacgcca gtccggccag atcttccatc
    22381 ccctttatgc cggaatacat atttctgaca ttaattgtca tcgccgctgt atcataagcc
    22441 ccagaagaat ttccaaaacg caccctaaag gtattttgac cgacatcagc atccgatgga
    22501 attcccgaca gcattccatt gggcaagacg gccagccagg ctggcccaga ttccttgctg
    22561 aaaactccga taccaccggt ataggctgcg agggattgcc cgacataagg tactaattcg
    22621 acggcatcct gattgccgat tggatcggct gtaaatacag gattgcctat cgctgccctg
    22681 ccgttaccgc tgttgtaaag aatttcagca aatgtcagat catatggtac attatagacg
    22741 gcgacatcat caataaagcc ggtataatcc cccctatgca aaccattggg ggtatcccca
    22801 tagaaaccaa ttccggcata ttgtccattg taattgtttg acgcattggt caacagagaa
    22861 atggagccag cctcattggc attgagccaa gcacgcagat aaccagatgt accaattgtt
    22921 atcaacacat ggttccaccc atttacgaca ggcaaattgc cgaattccgg tgttgcgttg
    22981 tcaacagtca gccggcagac acccgacggc aacaattcga tacgaaacat tttagctcct
    23041 gacaccgttc cgggcttgct gccgaggatg caggcagtat tatcagaaga aggcaggacc
    23101 gtcggcttaa accacagcga gatagtcatt ggagcggcag gcggaaaaat ggtgttggtg
    23161 gccgattcag accacccggc aatcaatccg tctttggggt aagttaaatc cagacaccct
    23221 acacccagca gtttgacatc ggtatccaac cacgccgctc cgaacggatt gttcggattt
    23281 gaagcaaata cctttgcaac atccagaaaa tccacatcaa acagccagta atgcaaaagt
    23341 gatgccggaa gttccggact ggtcttaaat gtccacaaat cacccccgat aactgatccg
    23401 tcagcggtta tttcatcgat acgccagtaa tatctcgtat ccggcttcag ggctggcggt
    23461 gtaaacgagg tggctgcctg gatgccgcag ctggcaagcc cgtccgggga agcaccaacg
    23521 tagatatgat gactgacagc cgtgctgccg gcacgccagc acagaatcgc atcagccgat
    23581 gcgttgttat ccccgctggc tggagcagga accgtggcct ttgtcggcca gagcgtcgtg
    23641 gctccgacta gaattgtagt gcccgccccc tccttaacga tggatgggat attctccgcc
    23701 gcatcaagcg tgtagggata gggcggcaca aatgcctgac cgtacgtctg catgctgcct
    23761 gtgcatccat cgaagatatt gccgacagat ttgatctcca ccgtataggt cccgccggaa
    23821 ccgacattga cgatgccggc atctctcatc ggattgttca cgtggtcaaa gtagttgctc
    23881 tcaagaagca ctttgaagcc ccatgacccg ccgatacagt aagaattgcc ggtgcaggag
    23941 taatagttat tgaacacgtg atcgctgccg taacggccgc gcggcatgcg ctggtccaca
    24001 tatcttcccc accagttgtg atggaaggta acgttcagct tgcctgcatc ggtcgggttg
    24061 tcatcggttg aaccgacaag acaggcaaac ctgtggggat taatcttgga agtataatag
    24121 aacctgcacc aggaaactgt aacataatcg cattggtcgg taatatccag caatccgtcg
    24181 gcagcgtcta agaaggtaca atggtcaatc cacagatgcg tggcgcggcg gcagatcagg
    24241 ccgtcttgcg gcatcccacc ctgaaaggta atgttgcgaa taattacatt atcaacatca
    24301 gtgaccttga cgctgaatcc ggcgattcgg gcatcatatc cgatgcccac gatagtctta
    24361 ttggccccga cattggggat attgtaatcc ccgtgaatgg tgccgactac ctggatgatc
    24421 cgcggggtat tgccctcaac agcgctgata aactccgcct tgtttgtcac tgtcacaacc
    24481 tgaccgcctt gtcccccggt cgtcccccca gcggcagttg cccagcctat cagctccgtg
    24541 ccaaaggata aggaccgcag gctcaaaagc acagcaaaca aatatccaac accggttaaa
    24601 tgatgattac ccagcattat cattgctcac tcttaatata cacattactg actgtcagaa
    24661 ctataactgg cgtcatttgc tgtcggtctg ttttgttgta gcaaagaatt cgacatcgtc
    24721 ttttcttggc gcaaatctac cgacgtggcc aacgcaataa cgcgtccctg gtcaccaacg
    24781 gcacagtaaa aatgataaac aataccgtta tgcttaataa gccacggctt atgtgcaaaa
    24841 gtcttgtcat aagaggtgct cggttcgatt agattagggc catcccactt tgtccagtga
    24901 acaagatcat aggaacaggc aaaggtgtca aaagccgcac tcttattgcc cggatacggg
    24961 tcccagaatg caccaaaata gaacataacc cagattccat taattcggac tatctgcgga
    25021 tctccgctga tcttgtgggt cggccccccg ttgacgatta ccgggttgtt tttgtggcgt
    25081 ttccaattgc gcatatccga agatgtggcc agaccgataa attcgctttc gctgcttccg
    25141 cctttggcgt tgtagaagat gataaaacgc gcatccggac caagggttcg attgggatct
    25201 tcgaaaataa aacttttgta cagggtataa tcttcccacg cccccgcatc cggatcggtc
    25261 actgacagca tcggcgactt gtccttggtc cagtatacca cagcgctggg ttcggttgca
    25321 gaagcccgac cgatagaaag cggagcaatt tcataacctg taatcgctcc gccgaagtaa
    25381 gaaagccagt acttgccttc gtagatccca agatgtccct tccctcccca ttccgtatca
    25441 gcaagggcaa tcccgccggc ggcattggcg taatcccact cgcctagatt gccctgaggc
    25501 aaaatggtac ccagacgctc ccaatgcaaa agatccgcac ttctggccag ctgggtggta
    25561 tagccggccc gatccttttc actctgcaca tgcatcatat accaatagtc accatgccga
    25621 aatacagttg gacaatcgat gttcctgttc tgctccgtat ctctgtgaat taccacacca
    25681 tacttgtacg gagttttgac ctcattgtaa atcgcttcca tcctaacagg gtcaaccgcc
    25741 gcagtctggg ccattgtcca aaggcaaaaa acggaaaaca cgacaagaaa aaaataggat
    25801 ccccgcattt tctgcttttt agatattact ttcattgcca ttatccaagc cctaaatcca
    25861 gaacagtaca gtggtgcgag aactgccgaa ggtactaaaa aatcctattt cagaagctct
    25921 tcgaatgccg atgctgctgc gatgcatagt aaaacagccg atgaacctct ctgtcatcga
    25981 caaactttcc tcctgtcata acactacgac ttattttaga tttactctgt ctttatatag
    26041 ggttttattg aattaagtaa accccctgcc gtcgattata gatagatttt aactatatta
    26101 acaaagatgt ctctacctca tttgaccaaa aaagttatta tatacataga tgataaatct
    26161 ggcagcggcc taaaatagtc agcgcaccgt cttttcggct tctacttttc aggttttttt
    26221 gactgcttca aatattttat gtttaattgg tcttgtttta ctgtaaattt ttcttttatg
    26281 cttttaggag tatatgcaga tatttttggt attctttata ggcatcgctg gcatacatac
    26341 gatcctggac cggagaaaag acagtcttat cttgattaag cttggccagt acagcaagat
    26401 tactaaaaac accttcctgc agacctgcca ggtaggcagc gccgagagca gagacttctt
    26461 ccattccaat attggtaacc tgtacattca gcaaatcagc cagaaactgc atcacaaatc
    26521 tattagctgt aataccccca tcaacgttta aaccggcaag ctggatgccg ctgtcggcct
    26581 gcattgcatc aattacatct ttaatctgga tgggtataga ttcgagagct gcacgcacaa
    26641 catgccgttt atcagacccc aaagtcagac ccgcaatggc agccttaaga tccatccgcc
    26701 agtatggcgt accaagtccg ctgaaggcag ggataaaagc aattccgttg ctgtcaggta
    26761 ccgcccgagc catcgcctcc gtttcgcgac tatcaccaaa caggcccagc tggtcccgta
    26821 tccatttgat tgcagccccg catgtgacaa tgacgccctc taaggcataa tccaccctgt
    26881 cttgtgcact ccagcagata gttgtgacca tgccgtattg ggattgaact ggtttggaac
    26941 ctgtgttcaa cagaatcgaa cagcccgttc ccatcgttgc cttggctgtt ccaacatcaa
    27001 aacacccttc ccccatggca gcggcatggg aatccccgat cattgcgcta atcttaaccg
    27061 ctttctggaa aagtccctga aaatccgatg cgccatacag atacgcagat ggtctggctt
    27121 ccggcagatt cagccccgtc aatgaaagat gtttcagaag gtctaaatcc catcgaagtg
    27181 tatggatgtt aaaaaacagg gtccgcgaag cattggtata atcggtacaa tactgtgttc
    27241 cgttggtcaa tttaaagaga agccaagtgt cgacggttcc aaaataggcg ttgcccgatg
    27301 caacagcatc tcgaatgcgg ggattatttt cgtacagcca gatcagcttg gtgcctgaaa
    27361 agtagggatc ggcaaggagt cctgttttgg caatgatttc tttttcgaca gcactgcctt
    27421 tcagatgctc gcagatgtca accgagcgct tgcattgcca tacgactgcc ggacacaagg
    27481 gcctgccggt gttgtcccat aacaaaaatg tttcccgctg attggaaatt ccgcaggcaa
    27541 caatgtcgct gatttttccg ttttgtgccg taaattgacg gatacattgt cttgcggcgt
    27601 caagaacgct ttcatagatt tcttcaggat cctgttccac aaatcccgga cgggggtaat
    27661 acgaacgcag cggacttgat gccttggccg caatctgtcc ttggccgtca aataataccg
    27721 ctttggtgcc gcttgtgccc tgatcaatag caagaatata tggattcttc ataagccggt
    27781 tcctgtcttc tatattcaat cggcagccag cagctgtttt gctgtttcaa tgatcccatc
    27841 tttgtcgatt ttatagtatg cgcggatctg attgcgaggg cctgcttttg aaaaacagcc
    27901 ttccggaata ccccgtgtta tcagtttggc tttaaggccg cacttggcga taacctcaga
    27961 aaccaaactt cccagacctc cgtgcagaga atgctcttcg acggtgataa cccgcccggt
    28021 tttcgcaata gatgcggcta tggaatctct atccagaggc ctgaccgaag cgatattaat
    28081 aacttcagca tttattgaat cattctgcag tgcttgggcc gcaaagtatg cctcataagc
    28141 cgtagaaccc attgtgaaaa tagaaagatc cgagccttct tggagtatat ccgccttgcc
    28201 gggttggaac cgatactctt ctttatggat gataggaaat ttgtcattgt ccatgcgaat
    28261 atagacagga cccttccgcc gaagggcata ctcaaatatc tgctttgcat caagcggatc
    28321 ggacggtgcg agaatgacaa tattgcccaa tcccagcatc atggagatat catcaatggc
    28381 atgatgggtg cttgctaacg gtccgtagca aacacctgcg ttcaatccaa ccaatttaac
    28441 attcgtatcg gagtaacaga tgtcattttt aacctgctcg gcggctcgcg ttgtaagaaa
    28501 gcaggccgca ttagccgtca cggcgacgaa tccccccaac gcgaggccgg ccgcaattcc
    28561 aaccagattc tgttcggcaa tgcccacatt gattaatcgt tcaggaaagc gctcctgaaa
    28621 tggttctatc ttggatgtgc tggttgagtc gcttaccaca attgccagat tggtcttgtt
    28681 ttctaccgct tcgataaaag tctgcaccat gacgcatcgc aagtcgtatc gctgctgttg
    28741 gatcattact gaagctcctc aatcgcccga atcgtttcct ccctgttggg aattcggtga
    28801 tgccaggctg gttggttttc aataaatgaa acgcctttgc ctttggtcgt ctgtgcaatg
    28861 actgctttcg gcttctgatt atccatgttc aatccctcga ttgtctggat aatagccgcc
    28921 ggggaattgc cgttggtgag atgaacatca aatccgaatg ccctgaattt gtcagccaaa
    28981 ggttcaagag ccataatcgt ctctgtgcca tcggcaagct gcagcttgtt tctatccaca
    29041 atcataacta aattatccaa atggaaatga gccgcacaca tagcagcttc ccaattggat
    29101 ccctcctgca attccccgtc gccagtcagc acaaaaaccc gaaagctgcg atgattcatt
    29161 tttgcactca gagccagccc cgccccgaca ctgagaccat gccccagtgc cccagtattc
    29221 aactcgacaa aagaacattt ctgtcgaaca ggatgccccg gaaggtccga atcaaactga
    29281 aggtatgtat caagcttttc ctcaggaata aatccggcct cggcaagcgt acagtacaat
    29341 ccgcaaactc catgaccctt gctcaggata aatcggtcgc gattcggatc atccatatgc
    29401 tccgtatcga tatttaagat gcgaaaaaaa agacaggcca gaatatccgt ttccgacagg
    29461 tcgctgccgg tatgtccctg tccggcatgg acgttggtct gaattatctt ttttcggatt
    29521 tcttttgctt tttcagcaat tttgtccgca gataaattga atggattcat tatatttctg
    29581 accgttgtga taatagatgt cccatttcat atagtttgat actgcctcat aaatgactct
    29641 ggcgctaagc ccccgagcca ttccgacatg gtgctcatag ccgttttgac agatatagcg
    29701 aagcagctga cgcaggttgt taaccttgca gacagcaatc ccgccatcca ttccgaacgg
    29761 atcgttcgta aatactcctt ctcccagata ggatttaatc cgccctttgg tatcatctgt
    29821 ggatagtcgg aagaaagtca taggccccgg taccgctttg cctttaacag caccaaagca
    29881 gtttttacgg tctaatacag tccccagtac atccaggttc gatatctcaa tctcgctgcc
    29941 tatgaaatct ttggggtagt tgctgcaatg tgtgcacacg cacatatcac gctggtttcc
    30001 ataattgttg ttccaatcca gcagcgcggg aatattgcct ccagccagca gcagggtata
    30061 catcgaaatg acaccggtaa catccatttc acaggcactg ggcatatagc actggcccat
    30121 catggacatc gacagacacg cggcacagcc gtaattgttt tcgacagaat tccagcattg
    30181 gatcgcgctt gcatccaact cgttttcctc catcagtcta tccagcacaa ccgataattt
    30241 ggcttgtttc acaatcgcct caggagctat ctctttgggg attctgccgt aatctttgat
    30301 ggccgccagt ttgtttttga cagccgcagc tgaagaatcc agcgcttgag cttgggcaaa
    30361 gatttcagat aaatcgatcg gcaccaccgt aattcctgcc gcctgaagaa gtttttcgct
    30421 gtaacgtacc gtctgaaacg ccgccggccg ggtcccaaat gctccaatgc gggcattttt
    30481 aaggccctta acaacccggc agacaaatcc gaagtatttc aggtcttggg caaaaagctc
    30541 actgtcaata tcgcaggtgt gctcggtggt gtcggtaaag gggatcttat attggtataa
    30601 attattgcat acggatatct tgccgcagta tgcatcacgc cggctcttta catcgacctt
    30661 gctgatttca tcattgcagg cctgaaccag caccggcaga tcgagctttg ccaactgaag
    30721 cgtttggata atacccaatt catcgccaaa attcggcaaa ataatcagga ctccatctat
    30781 caaatctcga tgtgccttga acaattcagc acagattttg gagtctgccc gtgtttcaat
    30841 tgctcctgca cgagtggcct tttcgtccgg aataacatac ccgaatccaa gccggtccag
    30901 ctggttcagt attttttgcc tttgttcggc ggcaagatgg gcattgaaaa tattacgcgt
    30961 gccgataatg acaccaaaag ttattttctt ttgcattatg ttcatacgac ctccttaagt
    31021 tctgtcaagt cgcccatact gatattatag cgccatacgc gatggatcaa gacactgaat
    31081 atattatacg ttccgtacat acgattggct gcagcagtat cagcacattt gtgcaaagca
    31141 taaacagcag cagcaaatcg aatatgtttt tgaaacaatt taaaaactcc ttttaccgca
    31201 gtcaacccgc caaacatttg ttcagccgta tgcgtggcca gatttagcgt ttgcgaccta
    31261 aaaccgattc ctttttatta caggccatga gatgacctgc agcaagatgc tgcctttggg
    31321 aactccctgc aaccattcta atatattctt caaactcatg ttcctgatca taaacacacc
    31381 acacatagtc ccacagattg aactgtatcc caacgaaatt gaccatccgc gccagcagac
    31441 ttttcagcca atttttatgt ttcttggaga acagcaatcg attgattcga tctcgacatt
    31501 atagatatag tatgaaattg gccagcattt atcaaatata cccattaaaa aaaaattgat
    31561 ttgggacttt tccccaaaaa accaatcctt cctgatttaa tacaactcat tataatcaat
    31621 ataattaatc tcagcagaat cgtagtctat ctgagtcatg cgataaaatc gtgttaaaaa
    31681 aaccatattt tttaagtcaa aaattatatt tttataaact ttggtgaatc aatatgctta
    31741 actgtctgca ctaatcatac aataaaaatt tacaagaaaa tagaatattt tcgcttgatt
    31801 tatataataa tctaccatat tcttaggttc atggtccatt ttgatcctaa acgcaaggaa
    31861 tttaccccgt atggttttac ctgtgaacgc tgggaaccgg ttttgatgta ccggccggac
    31921 aagcacaacg agattgaatt aaattattgt gatcaaggac agctaacata tcttttctgg
    31981 ggagaacggg tcactgtgga aggcggcagg ccagccgtat tctgggccgc tacgccgcac
    32041 caaattgtag actatgaaga tattgattcc tactttgtga tcacgctgcc ccttccttgg
    32101 gtgcttagct gggaccttcc aagtcccttt attacacagc tgctgaatgg tcagctgctt
    32161 caggaaaatc agcagctctc tgcaggatat cccgatcgcc agatatttga gcaatggtat
    32221 caggatatga cgcatcattc ggaacattgt catgaaattg tgctgcttga aataaaagca
    32281 cgtttgctgc ggcttgccgc taatttaatt gccagcaatg catttgcttc aggaacttca
    32341 aacagcagct ccaaaccggt aggaacgcag agtgtcagca aaattgagtg tatagcaaag
    32401 tttattgcac agaattacac aaaaaatata tgcactgctg atattgccaa ggaagtaaaa
    32461 cttcatcccg attacgctgc ggatctgttt cgaaaaacat ttggggttac gctgaatcat
    32521 tttattaccc agtaccgtgt tcagcatgca cagcgaatgc tggtaacaac aaataataag
    32581 attttggaca ttgcttatga atcgggtttc aattctttga gccgattcaa tgcttccttt
    32641 aagcagcttt gcggctgcac gccgcgtgag tatcgtgcag ctcatcaatt acacagcagt
    32701 taacttctga tacggttctt tataattgga gatttggaat atttgcggtt gcacactgct
    32761 aatttgaggt tggaaagcga taattaaaac aatctcctgc aatccaacag gacttatctg
    32821 caaatccgaa tctcctgaat gctatcaaga aaaaagattg ggaggcgatt gtgaagaaga
    32881 gtgctgtatt aagatggctg gatgctgtgc tagtagcggc ttttctgttc attggtctgg
    32941 tgcaagccca agaggaaaat caatccgccg cagtccagga gcccaaaccc ggcatggttc
    33001 ctcctctgcc gccgttgttg ccagctgaaa ttcccgcttt tcccggtgca tggggcggag
    33061 gcatgttcgc tacaggcggc cgcgggggca aagtcattct tgtaacaaac ctggatgaca
    33121 gcggccccgg aagtctgcgt gctgcaatcg aaaccgaagg accgcggatc gtggttttcc
    33181 gtgtagcggg catcatccag cttcaatcca acctcaccat tacgcatccg cacatcacta
    33241 tcgctggaca atccgctccg ggcgacggca tctgcatcgc acacggttca ttggacatta
    33301 atacctacaa cgtgattctg cggcacttgc gcgttcgccg ggggattacc tccggaggtc
    33361 aaggatcaga caatatcggc ggacacccgc accatcatgt gattgtggac cattgttcga
    33421 ccagttgggg acgggatgaa aacctctcgc tttaccgcgc gatgatggaa taccccgatc
    33481 ctcaaaatcc ggcagcaacg atatcggtca aaactcccgc cagaaatctg actattcagt
    33541 actgcatctc cagcgaaggg atgaaaccgg gtcatgaatt cggcgggact tggggaggcc
    33601 atgatgcgac gtttcatcat aacctgtttg catgcaacac cggacgcaat ccctctattg
    33661 gtatgagcgg ggagtttgac tttcgcaaca atgttatctt caactggcgt catcggacca
    33721 tcgacggcgg tgatgagaca tcgcttatta atatcattaa taactacttt aagccgggac
    33781 ctgcaaccaa tgacaatatg ttaagcataa tcgccagaat cgaacagcgt gatatgtact
    33841 cccccgggaa ccggttcaaa aactgcagct ggtatcctcc agccggcaaa cgcccgggca
    33901 aatggtatgt ggccggcaat atcgtagaag gatatcctga ggtaaccgct gacaactgga
    33961 agggtatgct gctgctggaa ggggtgggca ctctggatat ggcacgggtc aacagtccgt
    34021 ttgaaggctg gccggtgaat cagcagacag cactgcaggc attcagggac gtactggcaa
    34081 aatccggcgc cacactcccc cgccgtgatg ccgtggacaa acgcgtagtt gaaatggtgc
    34141 gaaccggcaa agttacagcc ggcaacggca ttatcagtga ccctaatcag gttggcggat
    34201 ttccggaata tacattttca cccgatcaag tcccatccga cagggactta gacggcatgc
    34261 ctgacgattg ggaggaaaaa tataacttgg atccatcttc agcctccgac agcagtcagg
    34321 atgcagacca ggatgggtac accaatatcg aagaatatct caacggcacc aatccccgcc
    34381 aaaaggtgga ctatactaat tttgataaca atgtcgatat tatcagctaa tggttttctt
    34441 ctgatgctgt ttaatttatc tgttccggct cgccttttac ggtgtaaatg agattgacag
    34501 gaaaggagat agccttgaaa catcacttgt cagccgtgat tcttgtgcta acagcggttt
    34561 taacagtttc atcggtagca aatcccctgc gcgtagatat caactgtgac agccgtcagg
    34621 atatgcgcac ggaggggtgg gaaaactggt gtcctgcggg cggggatatg aaacggtctt
    34681 tcggtaattt gactgtcagt ctgcgtgcac ccagcgggtc gattgaattg tccggcaata
    34741 aatcgctcgt tgtcaatggg attacggtcg gagctgacgg ggctgtcgcc gccggcgtca
    34801 agccggcggt tatggaactt cagatcgaag gactcgctgc cggtccgcat acgtttgtgg
    34861 gttatcatca cgccttccaa aacgccgccg gaatctactc tgtttccatt ggcgaccgca
    34921 aagcaggggg tattaaaacc acctccggcc aattacagca caacgatgag acagcagcat
    34981 cctttatcga attcgaagcc cgatccgggg aacccgttgt tgttcgtatc actgctctgg
    35041 atggtgactg cgttattctc aatggatttg cgctggatac ttcggaccca aacaaaaagg
    35101 cactcaagcc gcagccggca aattatgaac gacacgccaa tggcgacgac ggcaaggtaa
    35161 cattgagctg gacacccgcc caatcagcag tcagtcataa tatctatatg gcttgggacc
    35221 ttcaagccga tgccgcggtc agcaaattgg acaccgcggc aaaacccctc gcctcggtat
    35281 ccggcagctc ttattctgct tctatcgtac cacacaattc actgcttcac tacgcatgga
    35341 gagtcgattc aattgataac aaaggcaata tcacacgcgg ggatatttgg ctgttccgtg
    35401 ttcgccatcg tgcttttccc accgctgagg gatacggccg ctttgcaatc ggcggccgcg
    35461 gcggccgcgt gcttcatgta accaacctca acgattccgg acccgggtct ctgcgagagg
    35521 ccgttgaatc cacagggccg cgtaccgttg tttttgatgt ttccgggctt attagtctga
    35581 actccaaact cattttccgc agcgaaaacg aatatctcac cattgcagga caaacagcgc
    35641 ccggcaaagg catctgtctg cgcaattata ccttcggcgg catgggcggt cgcgatacag
    35701 tggtgcggtt cattcgcctg cgtctgggag acctgaccgg ccagacaatg gacggaatgg
    35761 gtcttgccgg ctgcaatgac tgcatcgtgg accactgctc aatctcctgg agcattgacg
    35821 aagcattcag ttcacgcggg gccggcaaca ttacgttaca gcgcagcctg atttccgagg
    35881 ctctcaatat tgcaggccat tcccactaca gcagcggaac cggccacggc tatgcaggct
    35941 ccatcggcgg gaacatcggc agcttccacc acaacctgct ggcccacaat gccggacgca
    36001 actggtcctt agccggcggc gtcgaccaag ccaatcgcca cgccggacgc cttgacatcc
    36061 gcaatatggt ggtctataac tggaaccatc gcactaccga cggcggtgcc aaagaagtcc
    36121 agtttgtcaa taactattac aagcccggcc cggcatcgca aatcctgacc taccttaatc
    36181 cgcaattcga aaatcctgcc tttggaccgc agcagtacta cgtggaaggc aacatgatgg
    36241 agggtattgc cggaccggaa ggtcccgaag gaccatttaa agggatgcac atacgtggcc
    36301 gtcaggatgc gccagtgaca gtttccaaac cctttttcga atcctatgtc agaacacaca
    36361 gtgccaaaga ggcctatgaa aacgtgctgg ctgatgtggg ctgcaatgta cccgtactgg
    36421 atgatcacga taagcgtgtg attcaggagg tgcgtgaggg taccgctatc tataagggca
    36481 gcaaatccca gctgcccgga cttccggact cccaagaaga tgtcggcggc tgggaagact
    36541 acccggaaat ccatcgtccc gccgactggg acacggacgg cgacggtatg cccaacaaat
    36601 gggaaatacg ccaacactta aatccccatg atcccacaga cggcacaatc gatccggata
    36661 gcgatggata caccaacctt gaagagtatt tgaattggct ggccgcaggt catacgctgg
    36721 attaatgggt gcggttatct tttatcggat gcttgcgcca gaaattggca agaatcgatc
    36781 cggatacatt catccacggg ctgaaaatgg ctgcggccag ccccaacgta gccagcttgc
    36841 ccaaatcgct ggcgatgccg aaagccatcc cgccattttg catcccgact tcgatagcca
    36901 ctgtacgggc atcacttttc tccagagcca acagccgact aagccagtag cctaacaaat
    36961 aacctatgag gttgtgtgct gccgccgcag caaacagcag cagcccgaca gtcagcagtt
    37021 cattacgccc cgaagcagtt gcgattgccg taaaatagat gattccgaac atcgacaatc
    37081 gcggcataaa actgtccaat ctgcgtattt tcagtgcaat aaaatgataa atcacacccg
    37141 ccagaactgc ggccagcagg aagctgacca ccgtgacgca attcgtcaaa acaggaactg
    37201 ggaaagtttt cgtgataact ttccagcccc ctcccgcaag gaacagcagc cacaaaccgc
    37261 cggctgcggc aattccatac acaaccctct ttcgagtatg tccggcatgc ttaagcaggt
    37321 cgtggaccat tgccgcgcca attggagcaa taacaatctt gaaaatggac atcatagcat
    37381 cgacgaactc aaaatttttc tcgatgccgg tattcagagt cgtcagccaa aaaggtgtta
    37441 taaaaggggc caatgcggta gcacaggccg tcagtgtgat cgacagcggc aggttgatgc
    37501 acgccatata cgccatcaca ttcgatgcca gcccgctgga acaggagccg atcagcacaa
    37561 gtcctgcacc gatttccggc ggaaatccga acacctttgc caaaaggaaa cccaccagcg
    37621 gcataattgt gaactgacaa actaaaccca cggcgaccgg gtacggcatc ttgactaccc
    37681 ctgcaaagtc ccggatgctc atctgggtac ccatcccgaa catcaccagc tgaataatca
    37741 tcaataccgc ccaaggatgg tttatattaa tcgaaaaggt ctcggtaatt tgcggattaa
    37801 ggaacacatg cgggacaagc agcggacaaa ttacagccgc tgcgatccat gcagtaaacc
    37861 gatagccgcg caggattgga acagcaccca gtcccaccgg cagcgctgcg gcagcaatgg
    37921 tgccggcacc cttggcgatt gcctcgttac tgcctgccca cgcaatcaat ccaacaagca
    37981 gagctgtaaa aaaaaatccc agcgccgctt tctgaaccgc ttccatcacg ccagctcccg
    38041 agccaaatgc tcaatcgcca gtctggggct ggcaaggacc ggcactgtca cttttgctgc
    38101 aggcaaagta ctgactacgc gtgccataga ggcctgtgct aaaacaataa catcaatctg
    38161 gtctgtcagt gcaaggatgc tctcagcgac gattttgtcg tgccttgccg catctcctga
    38221 aacaactgcc tcaaaggccc ctctgcatag tcttgcgatg atctcgatgt ttccctttcc
    38281 ctgtctctga agaagctcaa ccgttggatt taaggtagac ggcaaagtcg caatcacacc
    38341 gattcgcttg ccaatttcaa tagctttgct tgccatcggc tcatccaccc gcatcaccag
    38401 agattcagcc aattcacgcc ctacttctgc cgccctcccc aaggacgaac aggtcactag
    38461 aattgcgtcc gtccctgcct gatcggcaga cagaataaga ttgaccgcac ggcgggccgt
    38521 tgacttgctc agacagccgt ctttgagaat atctttgagt aagctttcat cgaccatatg
    38581 gaccgcttcg acatcagaca gcaattctcg gcagagctca ttaaacaccg gaaccatcat
    38641 gcctgaggta tgaatcaggg tcaggcgttt cttcttcatg cttttgcgtc cctccttttg
    38701 attgcggttt tggccgcatc gacaatatct tttactccca tatggtagta atccagcaat
    38761 ggttccggat gccccgatcg ggtaaagatg tccggcacgc ccaaacgaca cagatgaacc
    38821 ggacatgccg aaacactcaa ttctgcaatg gcgcttccca gtccgccgat aatattgtga
    38881 tcttcaacag tcacggcagc tgccgcctgt gacaaaagcg ggacaatccc gctatgatcc
    38941 agcggcttga gggtgggaac gtccaccact gctgccttga tgccttcccg agagagagtc
    39001 tcagccgcca gcaagacccg atttatcaca aagccgcaag caaagaggat aatatcacga
    39061 ccatagtcag ccagaatacg gattttgccc agctcaaagg ggacattctc gcagtccaca
    39121 atcggatcac gcccgctgcc gatacgcata taagccggac ctttgatttg gtaaattgcg
    39181 cgcgtcgcat gctttacctg ctctgtatcc gccggcacca ccacggtaaa accaggaatt
    39241 gtgcgcatga ttccaagatc ttcaaaaaat tgatgcgtta ccccttcacg ctcccctgct
    39301 ccgatgccgc cgttggcgcc gatcagcttg acgttaagcc ccggataagc cacaaaagta
    39361 cgtacctgtt cgcaggcccg catagtaatg aatcctgcat aggtggccac aaagggaatc
    39421 agaccgcatg cagccatccc ggaggcacag gccacagcat tttgttcggc aatacctaca
    39481 tcaatgaagc gatttgggta tttctgggca aaggattttc ccttgaccac cagcaccgaa
    39541 tcggcacaga ccagaacgac tttgggatcc tgttgggcaa gttcggccat cgtttcaatg
    39601 aaagcgattc gggtatttcc catcctcatc ataaataggg gcctcccaag atacgcatag
    39661 cctctcggta ttcatcggca gtagggacgc gcttatgcca cgaattgtcg tcctccataa
    39721 agggaatgcc cttgcccttg atagtgtggg ccagaatcac agacggtctg tctgtgcagg
    39781 cctgagcctt ttcaacggca tcgagaatct ggctaaaatc atgtccatca atctcctgac
    39841 aatgccagcc gaaggactcc catttgggca gaatgggata gagtccgctg atttcgtcta
    39901 ccagaccgcc gctttgtctt ccattgttat ccacaaagac aatcaaacgc ccggctttat
    39961 acctacaggc agccatcgct gcttcccaga ccacgccttc ctgaatctcc ccgtcacctg
    40021 taacaacata agtaaagtag tcttgaccgg tgatgcggct ggctaaaacc atgcctaaac
    40081 ccacccccac gccattaccc agcgaaccgg atgtactgtc gatcccggga gtcttgttca
    40141 tatccgggtg tccctgcagc atggactcta agcttcgcaa acccggcagt tcatcccgtg
    40201 agaaatatcc tttcccagcc aacgctgcat agagtgcagg acagccatgt cccttggata
    40261 ggataaaacg gtcacgatcg ggtttttcag gatgggccgg atccacattc atgacatcaa
    40321 aatacagggc tgtgattata tctgtcaccg aaagcgatgc tcccgggtga ccatcaccgg
    40381 ctttgtaaat catggtgata atatcgattc gaatttgccg tgcaatatcc tccaaggctt
    40441 tgataggcat attacgaatg gaatttagtt tttttatcat gctgctattt cctctcgaac
    40501 acactaagcc gcccgctttt tagtacaata tggagttctt tgttatgaat gcacctccat
    40561 tttctcttta atttgggtca taaaagttcg attgaccggg tacaggatta aaatcggcag
    40621 tgccgccgcc atcacaatcg gtccgactgt aaaagtaagc atagcaagct tgcgaatcac
    40681 atcgggtgtc tggttttcca ccccggacac atatcccacc caattcatca gacagccgtt
    40741 gacatacatc cccaccgcca tggcaaattt gagcacaaag ctgaacacag ccgaatagct
    40801 gccgtctttc agaataccgg ttttcagctg atgaatttcc gaaatatcgg caatcatcga
    40861 agtagacaac ggaatcagca taccgcatcc cccccaccac ataccctgaa aaataccaaa
    40921 cataataacg gaaaccggca agcgcagctt cccgattgcc caataggtct ccggcggcag
    40981 cagtccgccc aagaagataa tccagagcat tgcattcccg aatatgcaga aaaacacacc
    41041 cgcatagcca gcctgcttct tgtcgtatct gcgaacaagc tgtgcctgca gcaaagcgcc
    41101 cagcccaaag gcaatcatgc cggctccgtg aacaacggtc ttctgaaagg ccgtaaactt
    41161 cataaagtca acatatgtga acatttggac ttgtgcaacc accatcatgc ccaactgagc
    41221 aatcccgata aataggaaga cgtacagagc atatttatcg gaaatcgtct gagcaaaatc
    41281 cgacaaaaat gacttaaagc tgttgtgggt tttaatacat ctggtatcgg cggcataccg
    41341 gcgtgtaaaa taaacgcaat atagaatcaa aacggctgtg gttattgtca gaataatgct
    41401 catattgaag taattggaag caatatgggt cccatctaac cggctgccgt cctgattggt
    41461 cctgtccggg aagaacagaa tccatgccat agcaccgaaa aagaaattga ccgcctgatt
    41521 aacaaaataa cggacgctct gcagcctcga ccggccttcg tattccgtgc agatttcaaa
    41581 acccaaagcg gtgtagggca ccagaaaaac tgtcatcgct gtccgaaata tcaagttgat
    41641 aacaaccaaa tacccgaaca gaaaggccgg ctgtttgaaa aaatcgggaa caaaccatag
    41701 aaaaaagaag gcggctgctg tggctaatcc gccgaaaaac atatacggat gccgcctgcc
    41761 gaaacggctg cgggtattgt ctgtaagatg tcccataata ggatcagaga cagcatccca
    41821 aagcaatgtc agacccaatg ccatacccgc atatttagca tccatcccga gtacttttgc
    41881 atagtacagc atagcaaagt tattaacccc gttcatggaa atcgaagttg ccccttcgcc
    41941 gatggaatag tttacaatat ccttgaaagg agtaaccgat tggctttttt gttccatctc
    42001 ttgttttctt cctttttctg cagattaata gataggcatc cagactgtca tttcgccctt
    42061 gccccgattg ccccatgcat aatacggaat caagcgaata ttcaatggac gggggtcttg
    42121 ttctgagatt tcacgataca acacatcctg ccacggctgt ccgatccgct gcatcgctat
    42181 accttccaga accactacac cgcccagcag ggatgggtca aaccggcttg taagtttctg
    42241 tttccggccg attgcgatat ccatcggctt cacattatcc atcaggtcag ccgattccag
    42301 acaatacaca atggggcccc gcttgacagc agtctgattg cgggcctcct caacaaacgg
    42361 attggcctca atgaactgaa cgggcatcgg caaaacaagc cggacttggt caccggctgc
    42421 ccagcggcgc tgaatctgga aataagactc cggcttgggc atttctccag ccggtttgcc
    42481 gttaatctct actcgcgctt ctttcgccca gccgggaatg cgtaaaaata aagcagtttg
    42541 gtaagcgggg gccttttcca ttgttatttg aatggtttcc tcccaagggt aattcgtttg
    42601 ctgacgcagc ttgattggcg aaccgtctgc cagctgcgta tccaaggtat tgctgccata
    42661 aagatgaaca tataatcctg cttctgaaat actgtacgca tagcgggcgg attcggcaat
    42721 catccgtacg gtgttgggcg gacagcagaa gcagctgatg tagggctggc gctgccgtga
    42781 ccatcgcatt tcaaagggtt catcctgaac aacgcgaagg ggattggtgt agaaaaattc
    42841 ttttccatcc agactgatgg tggacaggat gccgttatag aatgccagct ccaaaatatc
    42901 tgcatagcgg gcatcggctg taatgtcgag catgcgctta ttgaagagaa caaaaccaat
    42961 ggtagcacag gtttcattat acgccgtcag attcggcagc tgatacgcac ggccataggc
    43021 ctgatggaca agctggatgg aatgatgatc tttggacccg tccggcgacg caccgtcata
    43081 gagcgcgccg gtagcgccat tgacatacat tttccgggtc acgatatccc gccagatgct
    43141 ttccaaaggt ttccataagg agtcattgcc cgtctcggcg aaaatgtcag ccgcaccggc
    43201 gtacaagtaa ttggcgcgaa cggcatgccc gaccgcctcg cgctgctggt aaaaaggaat
    43261 gcgatcctga ttgtcatcgg aaccttcctt caccatccag cggatatcga tcaggttctg
    43321 ggcaagttcc agatacttca gctgcccggt cacacgatac aaatcacaga gccccatata
    43381 atgcgaagga cagatggcat tatttgccag attttcgggg cgggtttggt aaaccgtgca
    43441 aagataatcc gcgctcctgc aggcaatctc aagcagcgtt tttttgcctg tggctttata
    43501 atgcatcgcc gcacaggtca tcaaatgtcc catgttataa gtctcgaagt ccattctatt
    43561 ttgaaactct gtattttgct ccgattgttt ttcggcgatg attaccggcg tgtggatata
    43621 cccgtccttc cgctgggctt ttccgattac gtcgatgatt tcatccatga gccgattcag
    43681 cttttcatct tttgttacag catatacgaa tgccgctgct tcgagccact tgtaaaaatc
    43741 gccgtcgtgc catttaggac cccggtgcct gccttcttga agacccgcgg caaccaaaaa
    43801 attggcgtag gcatggctga tgccggcatc tttcagaaga tcccacatgt tgggtatcat
    43861 cacttcgtga caaactctaa agcggtcggc ccaaaaaccc tccgtccatt ttacatcctc
    43921 aatgcccgta tccgtcagtt ttacatgggg ccggatcttg tcggctgcac caatcgcata
    43981 gacattcaga taaacagctg cacacagcgt atggataaac cataatttat tcataataca
    44041 atctcctcgc tgattcggca atttcctgct tggtcccagc tcgacagggt cggatgctca
    44101 gtttcatctc aatcggttcc ggcagcactt catagcgggg cagcacgccg ggaccgcagc
    44161 tgccgttgcc aaggccgctg tgacgcgcat ccagagacag gatgacctgc cggtggggtg
    44221 tcagttcata ggcatgctca gctctttcca aatcacctgc tgtataataa agagccgacg
    44281 cagcaatcgg ctgatctgca acaacaagca aaccatgccc ctgagcatcc gtcaatgaaa
    44341 gccagcgaat atcggttttg gtgccggttt cctgtggacg cggataggga acatactgtt
    44401 cctccactgt tccagaccag attcctactt cagcaccata gcagcgatcc ggatagttct
    44461 catgcggacc tcgcccatac cagcggaata tcgtgtaatc atgggcgata tgcataacaa
    44521 cacccagacg cggcagcgga ggcagctggc caaacggttc aaatcggctg tccatatcga
    44581 ttacgccatt tccaaacacg gtccaagtgg tttgaagcct aatgccgtta tgatcaggag
    44641 cagcaactgc cgatgttgtc ctgacttgaa tgtgcgtagg tccccgatgg ctgatttgaa
    44701 aatgttccgc tttacaggtc agctgattga gtccggcttg tttccaatcg taggcccgtc
    44761 ctttaccgaa cgctttatca ttgctggtcg gcgcccgcca aacctgcaga acgggtcccg
    44821 gcgggctttc ggcatcctga gacaatattt ccctgttatc ataaaccagc gaaatcagag
    44881 tgccctcaga gcggccgaat acggccctga aagcttcgcc ttggatccac acgccgtctt
    44941 ctttttcgat tacagttata ctcggcaagg tagcaatgtc aatttgtacg gggggtttcc
    45001 gttccgcaag cgctatctgc tcgaaggcga cttcgtgacc agcaggtgcc caaagagttt
    45061 tttcttttaa atgaaaactc agccgcagcc agatttcccc tttcgggtcc gaataatcag
    45121 ctgccggaac cggcagagtg atttctgtct gctgtccggg tgcaagatca atgctctgca
    45181 agatgccgga ttgctgtact ataccgtcgc acatcagatt ccatcgcggc tgcaatgtat
    45241 tgagattcgt gaagctgtat aaattcgtta aacgcaggcg gccgatgctg ttatcgacag
    45301 attcgatgcg taccggttga taaaccttct tgacttccca gactttcggc gtcagggtgc
    45361 ggtcggaaaa gaccagacca ttgagacaaa agtcctttga attcggctgg tcgccgaaat
    45421 cgcctccgta agcaaaatag tcctgcccga cggcagtttt cttccgcagc ccctgatcca
    45481 cccagtccca gataaagccg ccaatcatgc gaggatgtcc ataaatctcg tcccaatatt
    45541 cccgcagatt gccgacagca ttgcccatgg cgtgggcata ttcgcacatc aagacagggc
    45601 gatcatctcg cggatcctgt gcaatctgca ggattttcgg ccagcgttta tcgcgcaggg
    45661 aatcgtacaa ctcgtgaacc cgcggataca ttcgactgat aacatcaaca gtatccggat
    45721 cacgggggtc gtttttgtcc tgtgcatcgg cctgtgctcc ctcataatgg ataggacggg
    45781 tcggatcagc ttgccgaatc cacgccgcca gagccgcaaa atttggccca tagccggctt
    45841 cattacccag tgaccagaaa ataacactgg gatggttttt atcccgctgg accatacgga
    45901 tgccgcggtc caaaaatgca tcactccaag tgggatcatt ggccagatat ccaaaaagtc
    45961 catggctttc aatattggct tcatccatca catacagccc atactgatca cacaaatcat
    46021 accagcgggg atcgttggga tagtgggatg tccggacagc attgatattg aattgcttca
    46081 ttaaagttat atcctgcacc atccgctcca gtgacagggt acggcctgta tcaggatcat
    46141 gctcgtgccg gttgacgccg tacaggcgaa caggacggcc attgattaaa agctgaccgt
    46201 ttcttatctg aattcggcga aagcccaccc ggcaggaaac ggcttcgata acctcctgcc
    46261 ggtcagtatg taatgtcagg acaagagtgt aaagccgcgg gctttcagcc gtccattttt
    46321 ctggattttt tacagctccg ctgagccatc caaattcagc aggacctcgc tgaggggtcc
    46381 gctgctccag tattgctgcc tcgaaaccgg gattgagaat ctcgtacgca ttgcggctta
    46441 aggggctgtc ccacaccgga tttccagagg gatcatataa ttgagcttgt acggtccaac
    46501 cggccatatc ctgatcaccg cttccctgaa gctttggctg aatcaggatc cggccattga
    46561 tatagtccga atccagttct gtccgaacgg caaaatcggc tatccgcaca gcagccgtac
    46621 agtagaggta cacatcgcga taaatcccgc tcagacgcca catatcctga tcttcaagat
    46681 agctgccgtc tgaaaaacga taaacctctg cagcgataat attctcgccg taacgaagat
    46741 agggtgttat ttcaaattcc gccggcgtca tgctcccctc actgtaacca atccggatcc
    46801 cattaatcca gacataaaat gcgctggaga caccggcaaa atgcagaaat acacgccggc
    46861 cggaccagct tggaggcagc gaaaatgtcc ggcgatagga accgaccgga ttgcggttgc
    46921 ggtaggttgt ccaccctttt tcaggagtac ccgttacacg ggggggatca accttgaagg
    46981 gatattgtga gctggtgtag ataggaatat cgtaaccgtg catctgccag ttagagggaa
    47041 cagcaatggc atcccaggat cgaacattat agtccggccg gtaaaaatac agcggccgtt
    47101 tctgaggaca aggcgaccaa tggaatttcc ataatccgtt caatgacagg taatacggag
    47161 aaagctcagg agtgcagtta cgagcggccg cttcatccgg atagggaaca aatgcggcgt
    47221 gagcaggcaa gcgattgcgc tggttaacct gctgattttc ccaatccggt acagcaacag
    47281 actttacagc tgtttgcatc cgattgcagg cagctgcagt taataccact atcagcaagc
    47341 ttgatacttt taagattggc tgaacgtaat tcatgttcta tttatcgctg gtgttggggt
    47401 atcggcactt aaactggtaa gcaccggaag gcacttcaaa aacagcaaat tggccgtcgc
    47461 gtcccagtgc cttaatttct ggtatccctt ccactggtct gccgctttcc agaataggag
    47521 ttcgagggac actgggcaga taaacacggc cgcatgtatt agcaggaatg gcgacatcat
    47581 aggtgataat atcgccgtct ttctgccatc tgcttttaat cagtccgtgt atagagtgat
    47641 attccgctgc cgcataggtt aaaccgcccc ccggaaaggg acgaagcagc acttttttaa
    47701 atccattcag atgagaatcc agttcgatgc ccgcggcaca tcgaaagagc cattccccca
    47761 ccgatcccag cgagtagtga ttaaaagaat tcatttgagg atcaaaaaat ccttttgttt
    47821 cggtccatcc gttccagcgc tcccagatcg tcgtcgcccc gtgaagcacc ggatacagcc
    47881 acgatggata atcctcattc agcagcagcc gccatgctac atccgcccgt ccaatggctg
    47941 tcaggactgg attcagatga ctgactccaa tgaatcccgt gcttaaatgc cagttgcagt
    48001 ttctgatatt ttcaaccaga tgttcagcag cccgttctct tgcctgctgg ggcaacagat
    48061 taaatgcaag cgccagcaga tatgctgtct gagtatcgac ggtcagtctg ccgtcttcca
    48121 gaagaaattc tttttgaaat gcctctcgaa catactcaaa catctcttga aagcgttcgg
    48181 catccagctg gcggccgatg gccttggcca tgtttgacaa tttcgccgca tcatcagccc
    48241 agtatgcggt agccagaagc gttttcatcg gcgactgggc ccggaatgtt gtatcggagg
    48301 ggatacaaag ccagtctcca taattgttgc ccagttcctt gctgcggata ccgtgtggat
    48361 tcgttcgccg gatatagtcc agccaggcgg ccatcgcatc atagtgacgt tctatgatac
    48421 gggtatcatc ataaacccgc cacagaagcc acggaattat aatcccggca tccgcccaac
    48481 ctgcagcacc cccaaggccg tcaagaccga cgaaattggc tccttcccgc aggcgcggtg
    48541 ctgtatcagg aaatataccc tccggcgttt gggcatcttc gacatcaatc atccattttg
    48601 taaaaaatgc agcaacatcc atattatagc tggccgtgcg tgaaaaaacc tgtgcatcac
    48661 cagtccagcc caagcgttcg tcccgctggg ggcagtcggt cgggacactc agaaaattat
    48721 ctttttggct ccagagaata ttgagccata gccggttgat gccggcatgg gaacattcaa
    48781 atcgcccggc aggagacagc gcggagtgaa taacgcagcc gataacggta ccgtccggcg
    48841 gctgggcatc aaggccgcta agctctatat attgaaatcc atgaaatgta aaatgcggct
    48901 gccagacttc tttgccccgg ccgcttaata tgtagacatc tgtggctttc gcccggcgga
    48961 gattttcggt atagagcgtg ccgtcaggat tgaggcgttc tccatgcctg agtgtgatac
    49021 gcattcctgc aggcccatca atggtcagtc tcacccagcc ggtaatattt tggccgagat
    49081 cgtaaatcca agtgccgggg gcgcttttgt gaaccgagac aggtttgatc gtttcaatca
    49141 ttcggaccgg ttccgatcgc tgtgcaacca gcggaacatt cggcggcggc acattcctga
    49201 cctccgacca tgctgtgtca tcgtaatccg gcttgtccca gccgaccaga tcgtagcggg
    49261 catcataaat ttcacccatc ataaaatccg atgacagaat tggtccggcc gcacatttcc
    49321 acgaattatc tgtcgttatg atctgcgttg ccccgtcagc cagcattatt tccaattgaa
    49381 tcagcaaact ggtttcaagc ccataccggc cgcgtgtttc ctgccagccg acatacccgc
    49441 tgtaccagcc gtcgcccaga acagcaccaa gaacattatc ccccttctga atcatttctg
    49501 ttacgtcgta agtgcggtag tgtatgcgtt tgtcataatc cgtccattcc ggcacgaaga
    49561 tatcgttccc gatgcgtctg ccgtttagat acagttccgc taaaccgcgg gcactggcat
    49621 agagggtggc tttgcgaatg gaattcctga ccgcaaaaac cttgcgaaac caaggcgggg
    49681 ttccgggttc ggtcagggtt ggttttattg cgtaaccggc gctattaagg taatccggac
    49741 ctgccgcaat ccagtccgcc ttccaatcgc cttcttcgag caggcccatt gtccagaccg
    49801 ctgcatccga taccgcagag atcccccggt catcccagat ttgcacctgc caataacagg
    49861 tctgtcggga agtcaaaggc actccttcat agacaatatg tatcgagcgg tcgtcttccg
    49921 tgcgtccgct gtcccataaa tcagctttgc ccgaagcgag caattctgta caagaggcta
    49981 cccgcacatg ccatgcagac tggtatgtcc cccgccgatt cgattcaaca atccaactca
    50041 atcggggttt cttctcatca atccccagag gattaatcag atattcacag cgtagctttg
    50101 ctactcttat cgtggtactc aacctaactc ctcttctatc ctttttgggc tccttctgaa
    50161 tcccgacaat cgctgtgaca aattagtttt agcaatcacg catttaaaaa tccaatatat
    50221 tctgtgaaaa tcgaaaaatc ctccacatat ttttgaaaaa attcagcaca ttcttagaat
    50281 tgctgcggta ctggaggggt gctataaaac agacaatccc tgttacttga aagctgtttc
    50341 cctaatttgg tatcaaacat atttgcgcag tgcagtcctc cagttcccga atgaggcggt
    50401 tttattgaaa tttgtctatg cggttgaatt ggagcagtga aaactgggct gccggcaaac
    50461 cgcattcctc ctatcaatta tggaaaacag gaatgggaca aaaccatcaa ttcaaacaaa
    50521 aaaatgctga atcagaagca atagaactgc cggaaatctt ctgctgcaat cggaacttaa
    50581 ttttaaggct ttcgaacttt aaaaagacct atttttttca attttcaata tatcagcaga
    50641 atgcgcaaaa ttattttttt tcttgcgcat atttgacctg actgatttat tcaagatgta
    50701 tatgagcaag aaatggtatt tttatccgtt tttgttaatt gaaatttata ccaaaaatta
    50761 cggtatcaat gcatcataaa tctatgaata cgataagtta ttctataaat ccggcaggaa
    50821 attacttttt ttcttttata taatgatttg atgtatgata atatataatt gatgtgacgg
    50881 agtatcacat actctttaaa aaaatgaatt tggaaggagc tgccattacg aggagattct
    50941 gagatgtggg atgatttcaa ttttagttac aatcttccaa aaacataccg tcggaaaatg
    51001 agggctttta cactgattga attattggtg gttatagcca tcattgcact tctgctgtcg
    51061 atcattatcc cttcactgaa tttggccaaa cgcaaagcag ccgccgcaat ttgtctgtca
    51121 aacgctaaaa atctatctgc ggcatggtat atgtaccagg aagacaatga cggccgtctg
    51181 gttaatccta atcccacaaa ggtcaagatc gatggaagtc atgatgatcc gcgcttcatc
    51241 ggctgggtgg tagatccgat ccggccgaat ggaaccatgt gcaacggggg agaaggcccg
    51301 aataatcctg ttacagatga cgatgaaatt cgaggtatcg aaaagggggc tctctttgat
    51361 tatttgaaaa gtccggatgt ctatcattgc cccgccgata atcagcggaa aagcaaaatc
    51421 gacggcagta aaatttttcg aacctactcc tgtccccgcg ctataaaaag agttacaaaa
    51481 ttcatgaaca tcaaagcacc ggggatgcgt tttaactttg tagaggaagc cgaaggccga
    51541 aattggaata tgggaacatg ggacttttac aacatggctg aaaatggatt ctgggaatgg
    51601 cgggatccga ttggttttaa tcatggggac agcggcattc tgggtttctg tgacggacat
    51661 gcagaggttc ataaatggca ggtgtcttac acgttagacc gtgtccgctt ctttttttca
    51721 aatccaagca ctctcaacta tggcagtccg gggctcaacg acgattatcc tcgtcttatg
    51781 gcgggggcac ctcctgccga aagcgacccg gacattgcct atgtagctac cgggtgggcg
    51841 gaccaatccc ggccaatgag cgggggatat tagttgtaaa atatgctgcg gacacctgat
    51901 gggctgcgta gccgaaataa gctcttcgca gcacaagggt aaagaagttt ttcccgacgg
    51961 ctcgtcgcgt atctttttta atcttccttc ttccacttct tcttttgccg ttattgcaac
    52021 cattgcaggg acatcaccat actgaggggg gtctgcccac aacctattac tgcatattga
    52081 tctggctcag ctgtaaaata ctcttgcggc gtttcgcctt ttctgttaaa catgatgatg
    52141 caaagacacc ttctttctga tgatgccgaa ttggctcctc ctaaccgatt cccagaatga
    52201 taaccagatt gcaccggaaa gcacaccgcc aagcccaaaa caggaagatt cagcagaaga
    52261 aaagagttga ctttttgctg gattttgctg atagtaattt acggcctctg cccatcaaat
    52321 ttgctgcttg ttcaaattcg aggccgtttt attgaatcct tgccctctcc catcaatggg
    52381 ttttgtttgg gcacatgaaa tctggccgga aagaaggacc tttatgagat accagaaaac
    52441 agctcaggca tatttgtata ttatcaatta tcttttgcaa ccggctttct gtgctgcaat
    52501 tatttgctgt tccggcatcg gctgcagccg tgatgtgttc tctggcatac aaaaaaatca
    52561 tgctgttacg atcggttcac ttcgctgtga gtatatgatc aatccgctgg gaatcgatgt
    52621 taaagttccc cggttgagct gggcgattca gtcggcggaa aatggcgttc gacaaaccgg
    52681 ctatcagatt ttggtggctt ccgaaaagtc ctttttgcaa aaggatatcg gagatatctg
    52741 ggacagcgga aaagtctatt ccgctcagtc tgtccatgtg gaatacaacg gacaggaatt
    52801 gcggtcttgc caacactgct tttggaaagt caggatttgg gaccaaaacg gcaaggtgtc
    52861 cgattggagc cccatggctt gctggtctat gggaattatg cagccggatg aatggaaggc
    52921 caactggatt cagcagcgaa atcctgctgc ggaaaatgcc agtaaaaatg accttgtgaa
    52981 ttctatatac cccttgccga tcttccgaaa ggacttttct gttaaaccca acataaaaaa
    53041 cgccatcgtg cacatctcgg ggttgggaca ttatgaactt tttctgaacg gtcagaaaat
    53101 cggaaaccat tttctcgatc cggcctggtc ggtctatgaa aaaaccgttt attacaatac
    53161 atttgacatc acaagtatct taacaagcgg ggataacact ttcggtgtta tgctcggtaa
    53221 aggtttttac actactaaag gcgaccggcg tattcatttt gtaaatgttt atcgccccct
    53281 caaactgatt gtccaggcgc acattgagta tcaggatgga tcaaatcaga tcattgtttc
    53341 agatgattcc tggaaataca cgccgggccc cttaacgcat tgtgccattt tggggggcag
    53401 cagttatgat gcccgccgtc tgccggatgg ctggaaccag ccgggatttg acgacagcac
    53461 ctggcagcct gcccagcaaa catcaggacc gggcggacag cttaaggcat tcattgctcc
    53521 ccccatgcag acgatgcagg tccttcggcc cattagcgtt gatgaacccg agaccggata
    53581 ctttgtttac gatttcggtc agaatgtgtc atccattccg cgcctaatgg tggaaggcaa
    53641 agcggggcaa accattcgac taacgcccgc tgagcagcgg catggtcaga caggcacagc
    53701 caacaacggc aaagggagag tcaatcaagc ccgtataggc agcccgaatt attgggaata
    53761 taccctcaaa ggcggcgggc cggaatcgtg gtctcctcaa tttacatata ccggttatca
    53821 atatattgag gttgccggag ctgtacctgc cggcttttta aatcctgatg atttaccggt
    53881 catcaaagaa ttggtctcgg tgcaggtaag aaacgcctct gcgccggcgg ggacatttga
    53941 atgctccaat gcattgttca ataacatctg ccaattaatt gactgggctg tgcagagcaa
    54001 tatggcccat gttttgacag actgccccca tcgtgaaaaa ctcggctggc ttgaacagag
    54061 ctatctgatg gggtcggcta ttttttggaa ttatgacatc gcagcattct acaccaaaaa
    54121 gattcgagac attcgggatt cacaagatgc caacggcaaa atttacacgg tagcgcccaa
    54181 ttatcccgtt tttgaggatg ggccatttcg ctacagcccg gaatggggtg ctgcaggcgt
    54241 ttttctgcct tggcagattt atcaatggta cggagacagc agaattttgg aagaaaacta
    54301 tgacatgatg aaaagctatg tagactttat gcattcaacc tcagaagatc taatcgccct
    54361 gccgggtctg ggggactggt acgactacgg tcatggccaa tcgctcggac cttcccggtt
    54421 tacccccaaa accctgacct ctacagccac tttttacggt tgtgctgaca ttgtatccaa
    54481 aacggcaaaa ctcttgggca acacaaaaga cgcagaaaag tacacaatct tatgccgcga
    54541 tataaaagaa aaattcaatc gggagttttt cgacggcaga gggacctatc aaaacaacgg
    54601 aagtcctcag acggctaatg ccatggcact ggtgctcgga ctgacgccgc aaggcaaaga
    54661 agctgaggta cttgaaacaa tcatcaccga cctcaaacag cggcattacc agcagacagc
    54721 cggcgatatc ggataccatt atcttgtaca ggccctcagc gataacaatc gttccgatat
    54781 tctctaccat attacaaacc gaaacgaggt cggcagttac ggctacatca taaaacaggg
    54841 ctggacctgt ctgccggaag catgggatgc aacgctgact tcctcgatga atcattgtat
    54901 gctgggccac attcagcagt ggtttcagca gtgtctggcc ggcattcggc cggactttgc
    54961 cggcccggga tttaaaaagt gcatcatcaa acccgagatc acaggcgata ttgtatggtg
    55021 caaaggaact catgatacac tttatggaac gattaaatcc gcttggaaac tggagaacgg
    55081 acaatttatt ctggatgtca caatcccggc taacacctct gcaactgtgt atataccgat
    55141 gaaaaacgat tcgaaggtgg cggcagaatc aaacagcagc cgtaagggga ttcacttcag
    55201 tcatatagaa tcagacaaag ccgtctttga aattgaatcc ggctcttacc gattcaaagt
    55261 cccgtatgat tcataaaaag tcaatttgtc atttcgataa ccagaccaaa caggttggct
    55321 gcctgatacc gatactgccg gtgtaaagct tcagttggaa atggacaaat cctttaaaag
    55381 tgttattctt ttgtggacat ttttgaa
//
LOCUS       DAOTBO010000007        55091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_483_length_55091_cov_5.609801, whole genome shotgun sequence.
ACCESSION   DAOTBO010000007 DAOTBO010000000
VERSION     DAOTBO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 55091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55091
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_55091_cov_5.609801"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..2401
                     /locus_tag="PLE88_01600"
     CDS             509..2401
                     /locus_tag="PLE88_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM75192.1"
                     /translation="MMFPVNGHFHRIKSFSILGGFLDGLELDFDAGLNCLIGHRGTGK
                     TTVLEFVRYALDSFPSGEKGIVSRKHVESLVKNNLGDGRIRLTIETKDGLEYVIDRTA
                     SGDPMVLNADGTPTDISINTGGIFNADIFSQNEVENIADSPQSQLSLIDNFRATEISE
                     IYAQIATLKSQLQANANEIIPTQKNLFGLAEELNTLPGIEVKIAALAGPKDESTAHVN
                     KAHTDRALREREQHVFGELSDLLDKYKDWFDSAIGKFARQVNGLITADFLQGSNGPIF
                     QGIQADMVALGGRLDVLFNQGTDTIQTQQQLLEVKRQELSQLHDKQELAFRDLITKHT
                     QAQGVATERANWEKKRNDLLVKKRKSQQLQEQLQQLQTKRKDMMERLQTLYNQRFEIR
                     KTVAQWITENVSSPIRVQVDQFGDRSEYQNLLRDSLKNSGIQHGVVAKKIADLLPPNE
                     VVSILENGTAEDLETGAELNTSQAQKVFTALKSSDIFLTLDVVELADLPRIELKDGDE
                     FKNSLTLSTGQKCTTILPILLLDSDNPLLVDQPEDNLDNRFIYDTVVDSIRRVNKRRQ
                     IILITHNPNIPVLGEASQVFVLTSNGQHARVLNQGSVDACKEEIINLLEGGRDAFIQR
                     KQRYNY"
     gene            2401..3555
                     /locus_tag="PLE88_01605"
     CDS             2401..3555
                     /locus_tag="PLE88_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75193.1"
                     /translation="MEADTMFTQWPRLIKQLQDNPDSLPVSVRQQLADSLGQELLQGT
                     IACNETLFLELAEQLTKDPDWQVRLAVSRQLYLFDPTDCTDLIIRLCSDDNTYVRKNA
                     ERGLNRQVKFKQKRSKESQLSKDYLAQIKKLVGQYGPDIADKVHLLAEYRYSLLASTL
                     AHDFRSVLTTLSANVEALLKEHPRHKRTKSIQDDLVLLEQTIENMELFVKPLNVVKQY
                     ENIDEIIDAAVEKAKVKIAKLGYDISCIQLEVPKPERIRINISRPLLLLALTNLIKNA
                     YESFASDGQELKEGTIRIHVIVRGYEIQIIVKDDGPGIEPQILDKLSAFMPIDINKTK
                     RESTGFGVCTAYRYIVANNGTFEIDSQVDVGTTITMTFPLKDTDTPEETP"
     gene            3552..4655
                     /locus_tag="PLE88_01610"
     CDS             3552..4655
                     /locus_tag="PLE88_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM75194.1"
                     /translation="MKILVVEDDRKIKTEVIDDVLASLGYENDWATNQQEALEMLGNN
                     GYDLVLLDLQIPARSSSCAASSEFGKYLLRQIRDLKGHGNVPVVIMTGHHQDGLDMAA
                     DLIGMGVIDFISKPFPLKGRTLASVIEKTLQKYQKSKEAQPPKPEGTFTGGEMAFYPD
                     RIELCGVKIFGSKTVASREMLKVLAETKSDGTYRNLGGDQIAEKMDAENGIGTVTGCA
                     RTIRRQTAQILKKHLNVEIGENDLLEHSDQGYRLNHRITVRFCDQPENSWGHNDNLRG
                     QNSVPTDDLRGQKTDPINLGVSCRVKTRCLWILEELNKERKLRREDVEKDLNCSDKTA
                     QRALDILRNDGLITFEGSKKTGFYRLMEKQKIR"
     gene            complement(5043..6956)
                     /locus_tag="PLE88_01615"
     CDS             complement(5043..6956)
                     /locus_tag="PLE88_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75195.1"
                     /translation="MKSKDILRILENQIDKIVLGVIALISLFLLWVYVIGSPYSVEID
                     RRRLGPGDIDRYIKQKVEQALPAMEQPAVPVVYDKTYLADFDQYVRCAVPQLSTLSVP
                     YPGAGDIVIEEERMYALPQIPPLTDVAVAALRGAAQLPTEEIGPDRPYKQVVCELGDI
                     DLITVSAKLNVQNLYNNFMQSFKGPRLKSSWQDPVLAKPVFAKLELQRRVKQGDGTWG
                     DWETVPRAKIDAYKKLLEELPMTLEQAQLGVGIWRSQFENTNVQFDILQPEAYTFTIS
                     RTEWMPPKFLLEAFDILKKEEQAQERKRREEQSRQRTTPNVTDPTRPTPQRRQPVQPQ
                     RPQQQQTRPGTARRGGQDELLMGPGFENPALLRPEVRKERTITDVKNDMQKEILKEKD
                     NIEIRKDPLLVWAHDDTAVPGKTYQYRIRLGVFNPIAGKDWFQSDQVAFKNQTVLWSS
                     YTDPTPEVYVPKRVYVFPMEVILKADASKAVEGVKVEVDKYYLGQWRDFDFDVYPGQI
                     IGYEVEDVDKKTDPKTAPAVMGEFMPMMAGELGQPQKVDFTTDLMLVDIIQKALWNKR
                     PVYQMLYYDKEQRMQQIAVGRTNWSVDIRRDYEEIQNAKQQALQQLSPGFMQPAMPEM
                     MPIDPLLMMGPGA"
     gene            complement(7008..8342)
                     /locus_tag="PLE88_01620"
     CDS             complement(7008..8342)
                     /locus_tag="PLE88_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75196.1"
                     /translation="MNISMGFLKPVSGTLKKYTSLLPSVIIAIAALMMFIPTLWLGNK
                     VKGRMENSVRTARSVQSAVSQVPSKDQPAEVKRYMDKLEEEAAGIRTLAVQSSQRDLI
                     TYDYVIFPEPTDPSSQIYIGFGQRYRSRIEDLIRRMHALDAPSDAEIRARTGAAARPI
                     GEAYSPVRRPGTAQDPMVDALCQTRAQEISVYAHPKAFTWYDFWEKYSYTSKDQALQD
                     CWDSQVAFWIYEDIVDTIVKLNGTSAQVTNAPVKRLMGVSFSGPVVAAGVSSSYAASE
                     YYGYTAGMRDIPQYVTAAQPGIFVAASPTGRLCNEEMDIIHFAVSVLVDNRFVLAFMK
                     ELCSEKPHTFRTDFTKDGQLVESRHNQITILQSDVRVIDKSAAEHELYRYGKGAVMKL
                     DLVCEYQFNRKGYDAIKPAPVKKRLETASGMPGSGPAASSPAAPAGGLGFEM"
     gene            complement(8384..10711)
                     /gene="pilM"
                     /locus_tag="PLE88_01625"
     CDS             complement(8384..10711)
                     /gene="pilM"
                     /locus_tag="PLE88_01625"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOM75197.1"
                     /translation="MAAAAQGVWAIDVGTNALKALQMRHGDQGLEVTDFAYIEHSKNL
                     SSGDLQQEDKDQIIAETLRKLLDEKDLSKSEVAISIAGQNSFARFVKLPPVEPKKIPE
                     IVQFEAVQQIPFDINEVEWDWQLMEAEDSPDKSVGLFAIKNELIAEVMDHFTKEKLRV
                     TCVQIAPMALYNYAVYDLKELEQFPQKAVIILDVGAENTTLAVCSRDTIWQRSIRIGG
                     NTFTRAIADAFHKNFETAEKLKRTAPMSKYMRQIYTAMKSVYTDLGSEIQRSLGFYSS
                     SPEGRDKTFSKVIAMGGGMKLQGLSKYLTQTLGMQVIKPDTFERLAVSPDISVAKFHE
                     NVSDFGIAYGLGVQLLGEAKIRTNLLPRKIARAMAWTRKARIFTAAASVLLAVMVLGL
                     GRAYKDLKTYDSHQATRNEAAAILGQINAITGSIAEQQSRLQPLEDEIKKYMESFKYR
                     QAVPQFVETLVKCLPNKENTPEQKDLYEAVESGNVEGVLAVPRPERKLLFITRIAMEY
                     SDDLAKAKFPQPGTQDRTMTPRRRTPTMPILIDPMLEMMQPPVMPGGDMGIPGAPMPG
                     TQTEKTGMGFTLLIEGYSPYRKISELLDPVSVGEDQSRWGVVTRFENLAKLFPGIPFE
                     LFDRHDVAHFKVETGLVDLDSKTVPPGIGIIKEVERVPKPTSPTGVAAMPVYIGDYGV
                     GGRGADYVYTETVLVDPMTGEEISKTFDIITQKDISADPRLTDKDLGKKKFNPISGAP
                     QYIERDYWFRIQAKFVWKEAPPKPEGTAAGAMIGM"
     gene            complement(11074..11982)
                     /locus_tag="PLE88_01630"
     CDS             complement(11074..11982)
                     /locus_tag="PLE88_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM75198.1"
                     /translation="MRWIMWILAAVLTAAVCYLRYKKGGFMGVLLAIAVIAAAVYGGV
                     GLLLYLFQPAMVYQPSRTYDNTPADAGLVYESISLNTSDGQRLAGWFIPAGHNANECW
                     TILFCHGNAGNISHRLDTLKLFHQLGLAVLIVDYRGYGQSTGKPTEKGTLQDIRAGWD
                     WLIKKGIPARRIILFGRSLGGCIAALTAKDVNPAGLVLESTFTSIPDIGSHYYPWLPV
                     RLFSRFRYDTLTAVRQLHCPILVIHSPDDEIVPFRFGKTIYDAANEPKWFGMLKGSHN
                     EGFIENIDLYRHIWINWLTNLDTVNR"
     gene            12158..13189
                     /locus_tag="PLE88_01635"
     CDS             12158..13189
                     /locus_tag="PLE88_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75199.1"
                     /translation="MSKLALLILTVFSLVSCTALSAASGKKTGSPLDNLPPYMRQITH
                     FGERADWSGDGKKILFLERTFGDVFEVEPATGIIRPVTHHFFHEGFTRALYLANGDIL
                     LSGSRTFDAADPWASRSEKQAELWVLSKDLNAPAVPLGVHCSEGPAVSRKRMYIAWTQ
                     GNAFWGADIVYENGKPKLSGKTKLLDKKDLPFECGLETQNFRPPAEEELIFSAYDYQG
                     TEVCGLNLKTGKITNYSNAPNQYDEPEGIFPDGQYTLVECNKHMDIAEGIQEIDIYKL
                     ALDGSGRMERITYFADYIGYKATNPVVSDDGRFIAFQFAKKGDPAGVGRGILVLDMAA
                     FEKSLQERK"
     gene            complement(13502..14101)
                     /locus_tag="PLE88_01640"
     CDS             complement(13502..14101)
                     /locus_tag="PLE88_01640"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOM75200.1"
                     /translation="MAQNQGRFYIGSKLMTALAAGLLLAGILPAAWVIPQVLEGDSVV
                     YLQTTLPQTDPDTEDIEQSILPDEPSQISDNELLAMLITAEPSPAYEKPQETEHWQVM
                     RMRVTGYCPCSKCCGQYSDGRTACSHRIRQGDVFVAADKKFPFGTEVIIPGYNAGQPV
                     KVMDRGRAIKGNRIDVFFSSHEQARKWGVKYMDILVKAE"
     gene            complement(14865..16511)
                     /locus_tag="PLE88_01645"
     CDS             complement(14865..16511)
                     /locus_tag="PLE88_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOM75201.1"
                     /translation="MLAELKRYCLRLRTAVYRKPLTLAEKCRLQFGGAVLFSLVLALL
                     IPYFWMNKLTEKSALDAGRAITQAVYENHFRWTALDPQQLPRLSEVGEAITGAQPVIR
                     WLRFDNEGRIPAEEASESERQRLELLLKDASISDIAWTERTAEGGFKNHYLRLVLADE
                     NCLRCHFEQGPAPVFSKNQEIGLLIARTTAQGLAWTLFMNRLWVVVAGLLAATGAMVA
                     FYAITQRVILSPIRQLRALVNNIAEGNYDIRSAIKTGDEYERLSDAFNHMLDNLMESQ
                     HKLEKANQQLDAKISQLSEKNIELFKANKLKSEFLANMSHEFRTPLNAILGFAELIRE
                     KPQAEEEKVRRWAENIISSGRSLLNMINDLLDLAKAEAGKMSLHIEKTSIPQLLETLT
                     AFFSPLSEQKKIQVDLEIDPQLPLVQTDPHKVQQILYNLLSNAIKFTPEGGRVRISAA
                     MPETAIVRISISDTGPGISPQDQTYIFEKFHQLDGSITRKGEGTGLGLAICRQLADLL
                     AASIHLESEVGKGSTFSLDLPVNLKAKQEQPAEASSQTSG"
     gene            complement(16589..18406)
                     /gene="aspS"
                     /locus_tag="PLE88_01650"
     CDS             complement(16589..18406)
                     /gene="aspS"
                     /locus_tag="PLE88_01650"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOM75202.1"
                     /translation="MLKRTHLCGQLRLTDDGQAVILSGWVNSYRDHGSLVFIDLRDKT
                     GLTQIVFDQEQDAQMHRLARTLRCEWVITVAGKVRPRGQGLENPKLATGQIEVLACQL
                     EILNTAKTPPFEIDAANQVSEEIRLQYRYIDLRRSAMQEKLAVRHRITKIARDYFDEN
                     GFWEIETPMLGKSTPEGARDFLVPSRLVRGAFYALPQSPQLFKQILMVAGTDRYFQIV
                     RCFRDEDPRADRQAEFTQIDVEMSFVDSDDVMRMNEGLIRRIFKEIINVDLPNPVPRI
                     TYQQAMEEYGIDRPDMRFEMKLQNISDIAAQSTFQVFVSTVQKGGVVKGLCAPGGSKY
                     SRSDIEKTLTAFVSDFGAKGLAWMKVEKDDSGKVLPKSSISKFFTQDQLLELVRRFDA
                     KEGDLMLFVADAENAANKALAPLRVKLGRELGLFKPDDFKFVWVVDFPLFEWNEDEKR
                     YDSLHHPFTSPVEEDLGKLDTDPAHIRSQAYDLVVNGSEIGGGSVRIHNPAVQAKVFD
                     LLNISKKQAQDRFGFFLKALEYGAPPHGGIAFGLDRLTMLLTGTENIRDVIAFPKTQR
                     GQCLLTDAPSSVEQSQLDELNLRVQKHLHQVESDPDKES"
     gene            complement(18651..18977)
                     /gene="trxA"
                     /locus_tag="PLE88_01655"
     CDS             complement(18651..18977)
                     /gene="trxA"
                     /locus_tag="PLE88_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOM75203.1"
                     /translation="MAGNVVELTDDTFDTVVNGNGVVLVDFWAPWCGPCRMIAPVIEE
                     IAAEYAGKATIGKVNTDEHRQAAIQHAISAIPTLILFQNGQVAKKWVGLTSKDDITEA
                     IDELLG"
     gene            complement(19101..20966)
                     /locus_tag="PLE88_01660"
     CDS             complement(19101..20966)
                     /locus_tag="PLE88_01660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM75204.1"
                     /translation="MSVFDWKQKYSHLIQTAEEAMKLIKPGDSIFIGTGCGQPQHLVR
                     ALVEYGSHIYDAHIIHLLTMGDAPYADEKYREKFKMNSFFIAENVRGAFEQGIGDYTP
                     IFLSDIPRQFESGRIPLDVALISVTPPDVNGLCSLGVSVDIVKSAADNAKYVIAQVNQ
                     QMPRTMGNSFIHVNNIDAMVPYDEPIITVVMDPPNDVLRRIGRNLARLVEDGSTIECG
                     IGRIPQAMAEFLHDKKDLGVHTEMFGDWIIELIECGAVTCSKKTINNGKVVASFCMGS
                     QKLYEYIDNNPFFEFHPTEYVNDPFIIAQHDKMVGINVGLEIDLTGQVCADSLGYRFY
                     SGIGGQVDFIRGCARSRGGKPIIAMPSTAKDDTVSRIVPKLADGAGVVTTRGDVHYVV
                     TEYGIAYLHGKSIRQRVLSLINIAHPKFRKELIQAAKAQRYLQPDQIELEWDKVHYPQ
                     ELERFVTLKDGTEIFFRPVKPTDEAALADMLYSLSSESVKKRYFTHTKTFPHKDVQKI
                     TNIDYEQNLAIVGVVPGPEGAEEIVAIAQYFLDPKTQVAEVAFIVQDEWQDKGMGAML
                     LDYLTRIAKDRGVKSFYATVLPTNKAMLTIFYNSGYKINTEFDGDSYSIKYDLSK"
     gene            complement(21020..23131)
                     /locus_tag="PLE88_01665"
     CDS             complement(21020..23131)
                     /locus_tag="PLE88_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOM75205.1"
                     /translation="MTLDRFFNPQSVAIVGASRQPSKVGYEILAGLLKGGYPGKIFPV
                     NPTAPEIQGLKAYPDLKSIGQVPDLVVLVVSAKLVPQAVQDCANLGVKAVIIISSGFK
                     EAGPEGAELEKSIVRIAKTSGLRIIGPNCIGLMVPSRKLNVSFGGDLPQPGSIGYFSQ
                     SGSLLAAIVDMARDTHLGFSKLISIGNKADVDELTIIRALGEDAETEVIAGYLETIAD
                     GDAFIRQAESISRQKPILLLKAGFTAAGARAASSHTGRLAETESAYECVFERAGVIRC
                     ESIKDQFDYARAFAYQPLPKGSRVAVIANAGGAGIMATDAIEREGLQLAQFSEETRKR
                     LSQSQELSRAASINNPIDLLGDALSDRYEFALQAVLEDPNVDTALVLLSPHAMTECTQ
                     TAHAVVRVANQNGRNKPILACFLGAGRVAEAVRVLRDGKIPPYDSPESAVRAVKTMSR
                     YAEWKSRPPRVVKLFNVNRRKVERIIERHIRLQQAEVGEMEAKEILEAYGFVTPEGGI
                     AATPEQAANIANQIGYPVVMKVWSPDILHKADVGGVKTNINSDQEVLDVFDLMMYRIP
                     RKVPQAKILGIYVEKMCTGGREVILGMNRDPRFGPLMMFGMGGRLVEVLEDVVFYPAP
                     LTAEEARQMLVRTKTYDVLKGTRGQEGVDIGAIAEGLQRLSQLATEFPQIKEIDINPY
                     VVGREGTTPIAVDAMMTLEQR"
     gene            23344..23415
                     /locus_tag="PLE88_01670"
     tRNA            23344..23415
                     /locus_tag="PLE88_01670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23376..23378,aa:Thr,seq:cgt)
     gene            complement(23444..23629)
                     /locus_tag="PLE88_01675"
     CDS             complement(23444..23629)
                     /locus_tag="PLE88_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75206.1"
                     /translation="MRLCAKLTAWISLIVLVAPAVLFLANAITLETAKNVMLWATFVW
                     FIAAPLAMWKENGGKGD"
     gene            complement(23641..25134)
                     /locus_tag="PLE88_01680"
     CDS             complement(23641..25134)
                     /locus_tag="PLE88_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75207.1"
                     /translation="MGKTAVNADHTTNTCAQTQGAAQNSPQTESCHSDGFHPAAEYER
                     TPVPPHALLGFKSFVGMYAGEHCAGTELMIGPLFVASGISAFDMIVGLLVGNALAVLS
                     WMFLCAPIATRARLTLYYQLEKICGRRLVMVYNLANGVMFTILAGAMITVSATAFGIW
                     FKFPMPGLDDLYPNSIGWILAVAAAGTTIAVVAACGYKTVAKFANIAAPWMVLVFLAF
                     GLIGFRQFLNAADMEIQSYSDLWTLAKMRIWKGGPPLPGQVKFTFWHIMFFAWFCNMA
                     WHIGMSDLSVFRYARKSWYGVASAAGMYVGHFMAWICASILYAYQLHLNPADTDVLPG
                     PMTYRAAGLTGLICVIVAAWTTANPTIYRAGLAFQGIMPKKPLFWVTLVIGLVATVSG
                     MFPAVAMNLLDFVATFALVLMPMGAVIFVDFWLMDKLGLRSNYARLSGSQFNWAAGLA
                     WFITLAVCGWLVLSGRMQIYFVSLPGWFMTAVLYIVLSFIYQKKNPA"
     gene            complement(25127..26260)
                     /locus_tag="PLE88_01685"
     CDS             complement(25127..26260)
                     /locus_tag="PLE88_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOM75208.1"
                     /translation="MTTLLAAANPAVGIVIFMLGGLAGAVFYLPFKKVTKWAWESYWL
                     VYAVFGLVIVPWILALTTSPNVLSVLAGAPRNEIIKCFLCGAAWGVGGLTWGLMIRYL
                     GFGLGLAMGCGLCSAAGTLIPPMLKGSDAVSAMFKTPSGLVSVAAAVVSVVGIVFVGM
                     AGMSKESELSEEEKKKAVAEFNFKKGILAALFSGLMSSAMSFGLQGGPEIENLAMTTE
                     PHTSRAWAGMPVLVVVLLGGFVVNAVWCIFLNLKNKTAGDYVKKDAPVLANLVLAGLA
                     GAIWCSQFICFKTGEPMMGTTAYIGWAVLMASQILFSQLLGLMLGEWKGTSRKTQGLL
                     AAGLVLLIASAVIAGWSGYLGQSKSAGQSRNLPNAQQVSSIYG"
     gene            complement(26288..27553)
                     /locus_tag="PLE88_01690"
     CDS             complement(26288..27553)
                     /locus_tag="PLE88_01690"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF002203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOM75209.1"
                     /translation="MTKKTIEKAYSIAKEQYAQIGIDTDKVLKKLAAIPISMHCWQGD
                     DVGGFETVGAELSGGGIQATGNYPGKARTIDQLRADIEKAFSLIPGRHRLNLHACYID
                     NGGKYIDRNEMQPRHFQSWIDWAKANKLGMDFNPTYFSHPKAADGFTLSHPDKGIRNF
                     WIEHGIACRKIGAAIGRQLGSTTVTNFWIPDGYKDVPVDRVSPRLRLMDSLDKIFAVK
                     VNPAHNLDAVESKLFGIGAESYTVGSHEFYLGYAVSRQKLLCLDAGHFHPTEMISEKI
                     SSVMMFCPQLLLHVSRPVRWDSDHVVILDDELQAIAKELVRSGKLDKVHIGLDFFDAS
                     INRVAAWTIGMRNMIKALLIALLEPTETLRKIELKMDYTRRLAMLEELKTLPWAAVWD
                     YYCMSRNVPVGMAWLDEVKKYEADVLSKR"
     gene            complement(27587..29062)
                     /gene="rhaB"
                     /locus_tag="PLE88_01695"
     CDS             complement(27587..29062)
                     /gene="rhaB"
                     /locus_tag="PLE88_01695"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HOM75210.1"
                     /translation="MAEVHAYAAIDLGAESGRVVKGILQDGKLRLEEISRFPNGMVPI
                     GGQYCWNLVRLYESMLEAFKKCAGSDVPIESIGVDSWGVDFVLLARDETQMGLPVAYR
                     DSRTDGMMETLFKRIPSETIYEKTGIAFLKFNSIYQLLALAEKKSEALKNAAHFLMIP
                     DYFHYLFTGKLTNEYTDATTSQGINLKTKSWDEDIFKAIGVSPSILHPLIKPGTTIGP
                     ISSHLQAVTGLGPIPVIAPGTHDTASAVAAVPAEGKNWAYISSGTWSLMGIEADSPVS
                     SPKAFEYNFTNEGGVFDTIRFLKNIMGLWLVQRCRAAFAKELDYGTLTQMAAQAPAFA
                     SLIDPDRDMFMNPPSMTEAIAAFCRKTGQPVPASEGAYVRCCLESLALQYRLVMQQLC
                     EIYGTRIEKLHIVGGGTQNTLLNQLAADATGTTVITGPVEATVIGNIAMQAIGLGHIP
                     SLAEARKIIRQSFEMHTYAPKNTAAWEAPFERFMKLKKARI"
     gene            complement(29074..30147)
                     /locus_tag="PLE88_01700"
     CDS             complement(29074..30147)
                     /locus_tag="PLE88_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOM75211.1"
                     /translation="MDILDTITVLSHEFGTRRYVCGGGGNTSCKDAHTLWVKPSGTTL
                     ADLKPGDFIALDRHKLSELYTIEPPPDSAAREALVKEKMEKAVLPGISGRASVEAPLH
                     NALEARFVVHTHPSLVNGMTCSKNGSVVCRQLFPDALWLDYIDPGYTLCMRVRREIQT
                     YRQAYGREPALIFLKNHGLFAAADNAETIRSLYAQVMGRLEAEYAAAGISVDLQMGPM
                     PSAGQTDRARQLISAAVQDESVHIAASGFFEPAAGPLTPDHIVYAKSYPFVGRPSAES
                     IKAFQSRYGYTPQVMIFDEAVFGVSTSPKAARLALELAQDGGLVRQLAAAFGGVDYMT
                     DTARQFIENWEVESYRKNQLTKQ"
     gene            complement(30247..30924)
                     /locus_tag="PLE88_01705"
     CDS             complement(30247..30924)
                     /locus_tag="PLE88_01705"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOM75212.1"
                     /translation="MARYSRLQTLTVMKEIGLVPVFYNPNSETACRIIDACAEGGARV
                     VEFTNRGDRAINVFNKLTEYRDSKRPEVILGVGSICDAPTAAMYIAAGADFIVGPLLD
                     EETAKLCNKRKIPYCPGCGSVTEIHKAHELGVEICKIFPGGQVGGAAFAKAVKGPCPW
                     AELMPTGGISPTPESLKEWFDAGIACAGMGSKLITKELVAAGDFAGITRKVQDTLTLI
                     RQIRFVK"
     gene            31190..32182
                     /locus_tag="PLE88_01710"
     CDS             31190..32182
                     /locus_tag="PLE88_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOM75213.1"
                     /translation="MSRGEPLIRQWNLLKALQAYHYGLSADELACRLECSKRQVQRDL
                     NVLQEVGFPISYEQRDFGKRFWKLSHHFIESDKLIFSVTEMLSIFLSQKLLAPLAGTQ
                     FGEGLDSLFEKIKATLTPRALSHFTQMDSTLLVKSHTFSDYSAHDKTIRLLTKAIQDS
                     VVVNLTYKSQSRQKPYQTRYHPYGMVFFDGDLYCIGLMEIYGQIRTLKLNRIISSELT
                     PDRFRKPADFSIQEQMEGSLGIFSSLGPTQTIRIQLDGWAASSVREAIHHPSQKILDD
                     NDSRVIVQFKLADTTELKRWLLSFGCHACILSPKQLADQIASEIKKMSQRYTKK"
     gene            complement(32934..33296)
                     /locus_tag="PLE88_01715"
     CDS             complement(32934..33296)
                     /locus_tag="PLE88_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75214.1"
                     /translation="MTEFVPAESGNGINLKILAELISPQGFSIRAPFVMRFELYDYSP
                     VSSNPRGRQIILWPLQDLTDSDRNDQHWKEYLEGYEFLLPVETPLTSGKNYILEATCM
                     IGRRRLGDLFKIEFALNR"
     gene            complement(33308..33457)
                     /locus_tag="PLE88_01720"
     CDS             complement(33308..33457)
                     /locus_tag="PLE88_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75215.1"
                     /translation="MKIYKANDRGCFLPLWIVLAMAAGVILWDGRDVNCPAPQAPHQP
                     MSRPK"
     gene            complement(33444..34682)
                     /locus_tag="PLE88_01725"
     CDS             complement(33444..34682)
                     /locus_tag="PLE88_01725"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOM75216.1"
                     /translation="MSLFSEIPEILSELKQGRMIVLVDDEDRENEGDLVCAAQLTTPE
                     MVNFMATYGRGLICLPLSEERCDQLNLHPQTVENTARLGTAFTVSVDAREGISTGISA
                     ADRAHTIRVAADPASRPSDLVRPGHVFPLRARNGGVLVRAGQTEGAVDLMKLAGLEPA
                     AVICEIINDDGTMARVPDLEKYCRKHSLKMTSIAKLVEYRLQREQHIHRIQEIRLPTD
                     YGEFKMIGYSSPGTTEPHLALCKGGVGDLDEGGNPILHPEPVLIRVHSECMTGDLFHS
                     QRCECGYQLITAMKMIQQEGKGALIYLRQEGRGIGLTNKLHAYKLQEQGLDTVDANIK
                     LGFLPDKRDYGIGAQICRDLGLTHVRILTNNPKKISRLEVYGIKVAEQIPLMASPSEH
                     NRDYLRTKKIRFGHLLDEDL"
     gene            complement(34689..35309)
                     /locus_tag="PLE88_01730"
     CDS             complement(34689..35309)
                     /locus_tag="PLE88_01730"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOM75217.1"
                     /translation="MLFTIANQITILRVLLVAPFVICLIKTGDPSAGLYFRWAALGLF
                     GIAAGSDALDGFLARIRKQTTPLGTFLDPLADKLLITCACIILSLPQTAVEGFRLPLT
                     VVVLIIGKDILLLLGFVITYFLTGSIYIQPVWVGKASTAFQILMVLSILMGPEMARGL
                     EIWPLWTRIVWWITGLWALTATFIYIRRGIQYIESFGETAKSDSNR"
     gene            complement(35481..36473)
                     /gene="pheS"
                     /locus_tag="PLE88_01735"
     CDS             complement(35481..36473)
                     /gene="pheS"
                     /locus_tag="PLE88_01735"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOM75218.1"
                     /translation="MTLEQFEQIGRQAQEELDAVGTAEALEAYRIKYLGRKGLITEML
                     GMIGKLPPEHKKAGGQLANRIKNDITHAFEEARKRFETAGRKTAPMVDVTLPGIEVRQ
                     GRWHIITQTIAELLEIFGRMGFDVAYGPEVEDEWHNFIALNIPPEHPARDPADNFYIN
                     ESMLLRSQTSTIQIRVMEKQKPPIRVVAPGRVYRPDTVDATHMFMFHQLEALVVDEGV
                     SMIDLKSSIDQFIQTFFGAQTQWRFRPSFFPFTEPSCEVDILWVDKTGRQNWMEIGGC
                     GMVDPNVFDAVGIDSEKYTGWAFGFGIERLAMKKYGITDIRHLFENDIRFLRQF"
     gene            complement(36525..36881)
                     /gene="rplT"
                     /locus_tag="PLE88_01740"
     CDS             complement(36525..36881)
                     /gene="rplT"
                     /locus_tag="PLE88_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOM75219.1"
                     /translation="MPRVSKGAATRQARKRILKAVKGYRGATHTQYRLAKEARTNAMA
                     NARIGRRQKKRQYRGLWIIRLSAACRARNMRYSQFISGLKKAQIAVNRKMLSEIAIHD
                     PAGFDQIIEKVRAALA"
     gene            complement(36969..37163)
                     /gene="rpmI"
                     /locus_tag="PLE88_01745"
     CDS             complement(36969..37163)
                     /gene="rpmI"
                     /locus_tag="PLE88_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOM75220.1"
                     /translation="MPKLKTHKGLAKRVKITGSGKVKRHRCNHGHLLSGKSSKERRIS
                     GGQEIVKGVLAKKIRILLNK"
     gene            complement(37628..38494)
                     /locus_tag="PLE88_01750"
     CDS             complement(37628..38494)
                     /locus_tag="PLE88_01750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM75221.1"
                     /translation="MNTNHIGISIVAYHTEPQVLSDCLESVSHITFPFTCWVVDNSRS
                     ASVEAVVGGLGRRFPPWGHLEYMPSENSGYGGGHNRVLKIILEEGTSRFCLVMNPDVS
                     FERGTIETLVEAMNTDSTIGLLSPRFVFPDGTPQRLCKLLPTPGHLFKRRFLPLLAAE
                     YNHYYQLEDADYGAPFDCPNLSGCFLFMRTDVLRNVGLFDERFFLYFEDVDLVRRIGC
                     KYRTVYWPYATVMHHYRKGSYKDLRLLWNHLVSAVKYFNKWGWWRDSKRSAVNRETLK
                     VLNGQSKVPKEN"
     gene            complement(38499..40319)
                     /locus_tag="PLE88_01755"
     CDS             complement(38499..40319)
                     /locus_tag="PLE88_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM75222.1"
                     /translation="MIKYYIEFFKYFGKGYKRYIPLVIVGAAAAGLLEIAGLMLLLPF
                     IRILVRPESVRHHRWLMELMRLFGVQSPLQQACFLGGLIVFLFVAKNIYLLFYYFWQN
                     KILRRWKIEISTALMRFYLLAPYKLHLLKTTEKITRNVNHIAVHALNNFVLQGFLLIS
                     NIIAGLIILSVLITRFFLFAVITAGILTAASLCQYYFLKRKFKSLGAEKSALMSEQYK
                     NIFQGLHAIKETKVLGREQFFLDSFDGINRATMDNDMWTQFYHQFPHHLTEITAILCV
                     VVMCIGVIHSTLGDNPAMVASLGILAAIAFRTSSIVNRILKALQQINHSQHAVEVLLE
                     EVQSPLWRDYLQAAGGNSAGQDPAPLDFQKEIRFENVSYTYPGAKRPALCNVSVVIKK
                     GEYVGIVGESGAGKTTFVDILLGLLEPEEGRVWIDSAVLAPDTVRRWQRRLGYVPQQV
                     YIANDTVIRNVAFGLPNDQIDVEAVEKVLKMANLYDYVMGLGNKLETTLGENGRKLSG
                     GEKQRIGIARALYYQADVLVLDEATSSLDVPTESEIARSISSLKGRQTIIAIAHRLST
                     LKSCDRILYFDKHRLVDTGTFESLSEKYEKFRQMVEMSTL"
     gene            complement(40347..42932)
                     /locus_tag="PLE88_01760"
     CDS             complement(40347..42932)
                     /locus_tag="PLE88_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75223.1"
                     /translation="MNSKELYLHIGMPKTATSALQRFFSINRDLLAQKGIAYPAAGGL
                     EAHHRIGWALRLKRGFRHWWFDRDVGTCDFEWQQISRQCVLPRNLISTETFWGCTRED
                     VAFLKDLSRSYHTTVVVYLRRQDFFKESLYAEKVKMGVEKRTDVKIGAGWEKFALWER
                     TFGRDSIIVRPYERGQFYKGSIYADFLNSVWNAELTEEYKIPADLNLRLHRIVLEYKR
                     LVNHLPLSLQQIYAAVEPLQRISELFFQKGKSSDFVFSPALRRREIEKNRKFYHYLAR
                     EYCGRDDGMFFYEPEPSESDLWRPYRDLLEEDAAEINRCLADAAPTVLGVIAAGIVRA
                     QTAADPQMRLAARRLSPGIASEYFKADAVSAVLMPPIQTASQQTAAFDDLYASRMWEV
                     LQYFRPLYNRLPSNVIKCAVATAAAASRLTVRKKTGADMAGRSRLYVHIGMVKTGTSA
                     LQRFLGLNRELLQKMGIFYPKSPENLAAHHRLVWSIMTAQKVKKPDWPDDLQHPRREW
                     EQLRRQISSNSGILSSEHFLGFSGRQISQLKSLLRDYDVQIVVYWRRRDGLEDSWYNQ
                     RIKGGEITNRPQFLTNLPAKCCLDRWADVFGKEHLCVRPYERGQLYRGDVAADFLHHI
                     LGLELTGEFVLPEKEVNTRLHRVVVEYKRLVNLLPLSRSEKRSTVNPLRAVSDYMIQQ
                     GRKDYSVFSPAQRLELLARYAEESAAIARDYLGRPDGQLFYDPLPDPNEDWQPYEELL
                     EEDAKQINAYLEEHYPAALETIIQGLLKAFSAREENARQAALRLLPGIESQRIRSVLG
                     RYLVCFSKDVKAMAIEDGEMDMQQVRRAGMIAVRIYRRMPSFLQRPALRIVRRMEHKM
                     KSSVK"
     gene            43117..44265
                     /gene="glf"
                     /locus_tag="PLE88_01765"
     CDS             43117..44265
                     /gene="glf"
                     /locus_tag="PLE88_01765"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOM75224.1"
                     /translation="MNYDYLIVGAGLSGAVFAQTLAQAGHKVLILEKRSHIGGNCFDC
                     VDTAGVMIHRYGAHIFHTEFEDVWQYLSGFTRWRPYSHRVVCFVEGRYIPIPFNYYSL
                     QEAFDGKAAAIQCLLEQKYGRQTKLSVLDILHSQEPMLGNLAVYIYDKIFKNYTAKQW
                     GIRVDEISPDVIARVPIYTDYDNRYFTDTYQAVPADGYTAMIQALLSDPHIELRLNTP
                     FESVCQFTDGIIRLDGQRFNGILIYTGRPDALFGYRFGLLPYRSVHFEFETFAQSYYQ
                     HAPVINYPNDYNYTRIVEFKYLTGQKCPNTTICREYPRPCQTQADEPYYPILTPASVR
                     LYQLYAEQARQYDNLILAGRLGRFQYVDMDDAVKNALDAARTLLQCRV"
     gene            44253..45338
                     /locus_tag="PLE88_01770"
     CDS             44253..45338
                     /locus_tag="PLE88_01770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM75225.1"
                     /translation="MPRLTILIPVYNQAGYLPLCLQSIGEQEFTDFEIIAVDDGSSDG
                     SRSILDAYAADERRIRVIAKSNGGYGSALNAGLAAAKGDYIGIVEPDDTIEAPMYRVL
                     MAAAEQTQADIVKCSFRRIFSEGLCLEEKGWYAAAGRVFCLRQHPELCRLHPAIWAAV
                     YRRQLLEDNHIRFAETPGATYQDVPFFAEAFSAARTIFIVPQALYNYRLESENASSSS
                     NAIDAKVFYRLENHQRARRVYQARGLWEIVKYYELQREFITLNNFALRIKGSLRRALF
                     EKIRGYFAEIPVSEAVSFLPSGFRTSFELIKKGSYSRWMLQYALLPVIIIRLAEQLGL
                     SDRLRQKYYTWKDTGPAAYVQDPEYSY"
     gene            45310..47079
                     /locus_tag="PLE88_01775"
     CDS             45310..47079
                     /locus_tag="PLE88_01775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM75226.1"
                     /translation="MFKIQNIVTDNQMLQNCSRLFYTQEHRLGRSMLSFQTYFNTLWL
                     QAWKEYTCATRFYLNIRFRGQCSIRLIQSHLAEVKYDSASSSPLGEIASEHLFFEQTF
                     CSDGSDYQQIEITGHDAGARMVYFKIDHASDFEYIDAFYSVDSAFPPARVKTAIVICT
                     FRREQFVRRNLEILTAYFRKEPDLREVYDVFVVDNGRTLSADLAQYPVFLWPNKNTGG
                     TGGFTRGIIEALRSDRNYTHVLLMDDDIEIFPEALQRTYALAALLKPDWHRSFIAGSM
                     LRADMRHFQWEARSHIKGLQLEAYGRLFLSDFSSVVKNEILASRHTPRDYAAWWYCCI
                     PLAAIQPDRLPLPFFVRGDDIDYSLACADKIIHLNGICVWHEPFESRRNNVVDMYMTV
                     RNFTALSILHKSPLPLTVYHLMALFAGNIFTYNYKGAFLVCCGLRDAVRRICSFEDNQ
                     TELLDRYAAYNEIETNCAGDPSEAFKKIKLHVPVKLLIVLTYGGHLLPGFFFRKKTFA
                     LAGYRVAIAKCFASRHVCIYNPFTGKWIERKIDRLAAFALGFRFLFLLLYVVLFYPSI
                     RRKLLQKEAQYRTLEFWKRYLEL"
     gene            47266..48774
                     /locus_tag="PLE88_01780"
     CDS             47266..48774
                     /locus_tag="PLE88_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOM75227.1"
                     /translation="MNSRQLSDYIKVRQDSWRGYIQRDFADLEPGVLAGETPLDSRRS
                     ALERVASSHTAEVFRYAVQAGGRSYTLYLKKFPPRSPADWFKHLFRPSRAQRAFRAGL
                     LLRQYGFGTPPIAACLCRKKGLLMSQYILITQEMKQAHPLYLALESLCGLQDKRRLIA
                     ELGRTIGRMHAAGITHGDLRGGNVFVSQQAGPAVFFFIDNERTVRYPILPGWLRIKNL
                     VQLNMLEKNITSSDRMRFLKAYAAAAGLKKRTMRRLARAVIRKTQRRLRHRAKTRLGY
                     AEGPADHWNFQRTRWENRRGTFRMDFCKGTSAVAFLREIDNLTEHGVLLKNDTAARVV
                     RCTYNNRDIVIKRYNHQGLWHSLRYTFKGSRAKKCWRFGHRLTEAGIACAAPLACIEQ
                     YQCGLIWTSYIVNAFVEGPLLYDVMNRPGYSPQEKDAVMQQARQLLEDLGRHRLTHAD
                     MKPANLIIHHGKPVLIDLDAMQEHRMRLFFQYRYKKMVHYFHCRLHGKKRQN"
     gene            complement(48771..49748)
                     /locus_tag="PLE88_01785"
     CDS             complement(48771..49748)
                     /locus_tag="PLE88_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM75228.1"
                     /translation="MKLLILTNNPQRGSFKDRIGRYLDIFRAAGIEPTVAVLSSNPLK
                     RWGQYRQADTYDVVLLHKKCLNFLDARFFRPRKAKVIFNYDDAVMFNDKGCPTPAHQK
                     RFRRSLQKADAVLVGSSYLADQARPYHSNIIILPLGLKTDDYHPAAQKSDDGNIRLVW
                     IGSRATLGYIAELKPVLQKLAARYPGLVLRLIGDDFIDMEGVTIEKMKWTPHARGYCL
                     GQCDIGLAPLPDNPFTRGKCSFKVLEYSASGLPVVASPIGTNPDHVIDGQTGYLVRTE
                     QEWLEKLSLLIENPALRCQMGQNGIQQARNYDISAVGRKLCELIVKTAT"
     gene            complement(49780..50709)
                     /locus_tag="PLE88_01790"
     CDS             complement(49780..50709)
                     /locus_tag="PLE88_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75229.1"
                     /translation="MKAAHFLKLQNLHGISLQGLLAKRSVFEKDIIPIWRDFLLDDWP
                     VHIRLFEKYKVGFMEEPLWTGRSHETNTSRHIWKWFGPQIEVAARMAPMDLKAEAVGG
                     RIGSMARRMLKQNDNTHAIIRLALGTLMLTESPEQHKKAFRVLDKLTPRDKNHIIKTK
                     CQRLKESLNGLSSPSVQSFCTKTITWDNLGKEIAAVTAAGDPINRIGEIGKIFIRLAK
                     ECLSEGTNGQTELAVRTALAGIIMVEAGEDENSGTKILSSCASGGIRQLISRKIKLMK
                     SQSGFNLKSVFLLRSLFLLVTCLPILLFLSLGI"
     gene            complement(50737..51255)
                     /locus_tag="PLE88_01795"
     CDS             complement(50737..51255)
                     /locus_tag="PLE88_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75230.1"
                     /translation="MKCAMLVILSVTSLFAGCRSNKCNEIPFWDGKSIKDFEGKTMTS
                     MLWLLPPPYQDGVGYCLEKSRVFKRFDEADFKVLLSCLENPEIRKPVQEERLGRYLYL
                     SFLDGTRYIIDFKDSYPFPEKSGEYTIILPNGRSKILYNLLMEKEPCPTFELDPNMDK
                     YGRAKTPIPFAQ"
     gene            complement(51273..52043)
                     /locus_tag="PLE88_01800"
     CDS             complement(51273..52043)
                     /locus_tag="PLE88_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75231.1"
                     /translation="MEAIRDALIDKSIPYQELLYKNATWDNISIALQGGNLNYVYWVG
                     HMNSQIGEERDRITRKVLKEGVHRTNFKCWKIRRFWIDTDEDRIFSCLGSDGIIYPSV
                     PDTLPKDWEIRGHSMRSLGLWQTNKIKEFWAIGCESGLEWKNGVNTYNFNDMANAVGA
                     HYKDPQGNYVHVYMGNRTPVWTGWFLPEAEHYPAALAGIIRNHGNNHSLEHALINGPH
                     SNEQRAAVWGDDLNRDGWADNVLQWWPLNTKLDWIQFF"
     gene            complement(52112..52534)
                     /locus_tag="PLE88_01805"
     CDS             complement(52112..52534)
                     /locus_tag="PLE88_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75232.1"
                     /translation="MKLLILTNNPQRGSFKDRIGRYLDIFRAAGIEPTVAVLSSNPLK
                     RWGQYRQADTYDVVLLHKKCLNFLDARFFRPRKAKVIFNYDDAVMFNDKGCPTPATSA
                     IRRSSAPPPARKRPYPMKTAKTQRHRICLLWQSWICPR"
     gene            complement(52566..53939)
                     /locus_tag="PLE88_01810"
     CDS             complement(52566..53939)
                     /locus_tag="PLE88_01810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM75233.1"
                     /translation="MTGNNTPLVSVLVPYYNGRRYIREAIFSILNQTYPNIEIIVVDD
                     ASPNHDESDYIRKFAVEMGINLLVHSENKGIGPTLADAFEVTCGEFITGLSQDDLYKP
                     EKIEKQVRYLEENNLDAVYAVGDILQQTTGRLYQRDTTKTSQFIRSGKAAHFLKLQNL
                     YGISLQGLLAKRSVFEKDIIPIWRDFLLDDWPVHIRLFEKYKVGFMEEPLWTGRSHET
                     NTSRHIWKWFGPQIEVAARMAPMDLKAEAVGGRIGSMARRMLKQNDNTHAIIRLALGT
                     LMLTESPEQHKKAFRVLDKLTPRDKNHIIKTKCQRLKESLNGLSSPSVQSFCTKTITW
                     DNLGKEIAAVTAAGDPINRIGEIGKIFIRLAKECLSEGTNGQTELAVRTALAGIIMVE
                     AGEDENSGTKILSSCASGGIRQLISRKIKLMKSQSGFNLKSVFLLRSLFLLVTCLPIL
                     LFLSLGI"
     gene            complement(53926..55044)
                     /locus_tag="PLE88_01815"
     CDS             complement(53926..55044)
                     /locus_tag="PLE88_01815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOM75234.1"
                     /translation="MANEGDVMIPFLDLKAVNQRYAADIESVFRRVLDSGWYLLGNEL
                     SAFETAFAAYCRMKYAVGVASGLDALSLILRAYGFGPGDEIIVPSNTYIATVLAVTAC
                     GATPVFVEPDTQTYLIDSGRIEEKITKRTKAVMPVHLYGQLCDMAAICTLAGRHNLKV
                     IEDAAQAHGAVYSEDSITTDYPQRAVGYSFYPTKNLGALADAGMVTTNDGQLAEKIRA
                     LRNYGSHRRYYNKYPGINSRMDEIQAAILRIKLRGLDEDNRQRRMTAEYYLKNIKNER
                     VILPKPPQESSSHVWHLFVITTKWRDDLQKYLESNGIQTHIHYPVPPHKQECYPQFNH
                     LSLPIAERLAKEVLSLPISPAMNQNDIQIIARAINDWK"
BASE COUNT        13807 a        14587 c        12983 g        13714 t
ORIGIN      
        1 aagattgacg gattatggat gggttcgaca gaatccaaag tcgaaattct ataaattttc
       61 gttgtcggcg caaactgtgg gcgtgttgcc tatgaaaacc tgacgtttca ggcgacacgg
      121 gtataaacgt gatgcagacc gccaaggatg gacgttttga caatttgacc ttgcgtttga
      181 acagggcgag ggacgggcga atccttgttc agtgacatgt gtatacggct gacattgtaa
      241 tagttttcga taaaatcggt caatactggt agcaaatgtt ttgttcacaa tgattacgtt
      301 atccgagcat tcccggccaa gtatgtctat gactcgtttg gcaaaaattt ttcatctatt
      361 tttttcatca gggcattgac aaattttttc ccgtttatta ttcttgctga tttagtaagg
      421 ttcagggttg caacgagctc tggtttgtcc atctccctcc gaagccctcc ttagcgtatt
      481 cgtttaactt cattgaaagg aacggcttat gatgtttcca gttaatggtc attttcatcg
      541 catcaaatcg ttttcgattc ttggtgggtt tctggatgga ttagaacttg attttgacgc
      601 cggattgaac tgccttattg gacaccgcgg cacgggcaaa acaacggttt tggagtttgt
      661 ccggtatgcc ctggattcct tcccttcagg agagaagggt atagtttcca ggaaacatgt
      721 cgaatcgctc gtcaaaaaca acttggggga tggacgcatc cgcctgacca tcgagactaa
      781 agacggcctg gagtatgtca tcgaccgaac cgccagcggc gatccgatgg tactcaatgc
      841 cgatggaacg cccacggata tttcaatcaa cactggcggg atttttaatg cagatatttt
      901 cagccaaaac gaggtcgaga atattgccga tagtccccaa tcccaattat ctcttatcga
      961 taactttcgg gctaccgaga tttcggagat ctatgctcaa attgccacac tgaaatccca
     1021 attacaggcc aatgccaatg aaatcatccc cacccaaaag aatctgttcg ggctggcgga
     1081 ggagttaaat accctgccag gcatcgaagt gaagattgcg gcgcttgctg gtcccaagga
     1141 cgaaagcacc gcccatgtta ataaagccca tacagaccgc gctttacggg aacgtgaaca
     1201 gcatgtgttt ggggaacttt cggatttact ggataaatat aaagattggt tcgacagtgc
     1261 tataggcaaa tttgcccgcc aggtaaacgg tctgatcacc gcagactttc tgcagggctc
     1321 caatggtccg atattccagg gcattcaagc agatatggtc gccctcggcg gccgtttgga
     1381 tgtcctgttt aatcagggaa ccgatacgat ccaaactcag caacaattgc tggaagtaaa
     1441 acggcaggaa ctgtcccaac tgcatgataa acaagagctt gcgtttcggg acctgattac
     1501 caaacatact caggcccaag gagtcgccac agaacgtgcc aactgggaaa agaagcgtaa
     1561 cgacctgctg gtcaaaaaac gtaaatccca gcaattgcag gaacagttac agcaactgca
     1621 aaccaaacgt aaagacatga tggaacggtt gcaaacgctg tataatcagc gttttgaaat
     1681 ccgcaaaacc gtagcccagt ggattactga aaatgtctct tcgccgatcc gtgtgcaagt
     1741 agaccagttt ggcgaccgtt cggaatacca gaacctgctt cgggactctt tgaaaaacag
     1801 cggcatccaa cacggggtgg tcgccaagaa aatcgccgac ctgctgccgc cgaacgaagt
     1861 agtgtctatt ctggaaaatg gtactgctga ggacttggaa actggcgctg aactgaacac
     1921 ctcacaggca cagaaggttt ttacggcatt gaaatcgtcg gatatttttc tgaccctgga
     1981 tgttgtggaa ctggcggacc tgccaaggat tgaattgaaa gatggcgatg aattcaagaa
     2041 ttccttaacc ctctcgactg gtcagaaatg cacgaccatc ctgcccattc tgctattgga
     2101 cagcgacaat cccttgctgg tggaccagcc ggaggacaac ctggataacc gatttattta
     2161 tgataccgtg gtagacagca tccgcagggt caataagcgt cgccagatta tcctgattac
     2221 ccataatccg aacattccgg tactgggaga ggcatcgcaa gtttttgtgc tcacgtccaa
     2281 tggacagcat gctcgtgtcc tgaaccaggg cagtgtcgat gcctgcaaag aagaaatcat
     2341 taatctgctc gaaggcggcc gggatgcctt tattcagcgc aagcagaggt ataactactg
     2401 atggaagccg acacgatgtt tacccagtgg ccccggctga ttaaacagtt gcaggataat
     2461 cctgattctc tgccagtctc ggtacggcag caattggcgg actccttagg gcaggaactg
     2521 ctgcagggaa cgattgcgtg caatgagaca ctttttctgg aactggctga acaattaaca
     2581 aaagatccgg actggcaggt tcgtttggct gtctcgcggc agttgtatct ttttgacccg
     2641 accgactgca ccgatttgat tattcgcctg tgtagcgacg acaacaccta tgtccgcaaa
     2701 aatgccgaac gaggcctcaa ccgccaggtc aaatttaagc agaaacgctc aaaagaaagt
     2761 cagctgtcca aagactatct ggcccaaatc aagaaactag tcggccaata cggaccggac
     2821 attgccgaca aagttcatct cttggcagaa taccggtatt ccctactggc atcgacattg
     2881 gcacatgatt ttaggagtgt tttgacgacc ctcagcgcca atgtggaagc cctcttaaag
     2941 gaacatcccc ggcacaaacg aactaaatcc attcaggatg acctggtgct tttggaacag
     3001 accattgaga atatggaact ttttgtcaaa cccttaaatg tggtaaaaca gtatgaaaat
     3061 atcgacgaga ttatcgatgc ggcggttgaa aaagcaaagg tcaagatcgc caaattgggc
     3121 tatgatattt cttgtattca gttggaagta ccaaagccgg aaagaatccg aatcaatatt
     3181 tcccggccgc tgctcttatt ggcattaaca aatctgatta aaaacgctta tgaatcattt
     3241 gcctcggacg ggcaggaatt gaaagaaggg acgattcgta ttcatgtcat tgttcggggc
     3301 tatgaaattc agatcatagt gaaggatgat ggaccgggaa tcgagccaca gattttggac
     3361 aaattaagcg cctttatgcc catagacatt aataaaacca agcgggaaag cactggcttc
     3421 ggcgtgtgca ctgcctatcg atatattgtg gcgaataacg gcacttttga aatcgacagc
     3481 caggttgatg tgggaacgac tatcaccatg acttttccgt tgaaagacac cgatacaccg
     3541 gaggaaaccc catgaaaatt ctggttgtag aggacgaccg caagattaaa accgaagtaa
     3601 tcgatgatgt tctggcatcc ctgggctatg agaacgattg ggcaaccaat cagcaagagg
     3661 cgctggagat gcttggaaat aacggttatg atctggtact gctggacctg caaattccgg
     3721 ctcgctccag cagttgcgct gccagttccg aatttggaaa gtatctactg cggcaaattc
     3781 gggacctcaa gggacatggt aatgtccccg tggtcattat gaccggccat caccaggatg
     3841 gcttagatat ggccgctgat ttgattggca tgggcgtcat cgactttatc tcaaaaccct
     3901 tcccactcaa aggtcgcacc ctcgcctctg tcatcgaaaa aacgcttcag aaatatcaaa
     3961 aatccaaaga agcccagccc cctaaaccgg aaggcacatt taccggcggt gaaatggcct
     4021 tttaccctga ccgcatcgaa ttgtgtggcg ttaagatttt tggcagtaag actgttgctt
     4081 ccagagaaat gctcaaggtt cttgccgaaa caaaatccga cgggacttat cgaaatcttg
     4141 gcggtgacca gattgctgaa aaaatggatg cggaaaatgg gattggtacg gttaccggct
     4201 gtgcccggac catccgtcga caaaccgcac aaatcctgaa aaagcacctc aatgtcgaaa
     4261 tcggcgagaa tgatttgctt gaacattccg accaaggtta ccgactaaac caccggataa
     4321 cggtcagatt ctgtgaccag cccgaaaatt catggggtca taatgacaat ttaaggggtc
     4381 aaaattctgt ccctacagac gatttaaggg gtcaaaaaac tgaccctata aatttagggg
     4441 tcagttgtag ggtcaaaaca cgctgtttat ggatattgga ggagctgaat aaagaacgaa
     4501 aattgcggcg ggaagatgtt gaaaaggatt taaattgctc ggataagacg gctcagaggg
     4561 cactggacat tctgagaaat gatggtttaa ttacttttga gggttcgaag aaaacgggct
     4621 tttatcggct gatggaaaag caaaagattc gataacttct tgaaataacg tgttttgtga
     4681 caaatactcc tgacttgcta tgatgaactc ctgaactaaa ggttcaaacg tatgacagtc
     4741 gctccgactg tctttcagca ggtaaatgcg gggcatattt gcccgctctt ggctggtcct
     4801 gagggctgca ggattattga taattgccgg caattcaaac gaagggccgt cgtctcggtt
     4861 aattcttcag attatccgtc tatttaccgg cctgtttttc acagaccaag ttccctgtac
     4921 tcctgttaaa aaaacacttg tgccgcatcg actgttcatg cagcacaagt gttcctgcgg
     4981 gcagttgtaa tttcttataa tagaagccgg atcgcggcac cttgatatgc agctgactgc
     5041 atttaagcac ccggtcccat catcaataac ggatctatcg gcatcatttc aggcattgcc
     5101 ggctgcataa agcccggact taactgctgg agggcctgct gcttggcatt ctgaatttct
     5161 tcgtaatccc ggcgtatatc cacgctccaa ttggttctgc cgacagctat ctgctgcatc
     5221 ctttgttcct tgtcatagta gagcatctga tagacagggc gtttgttcca taaagccttt
     5281 tggataatat caaccagcat taaatcggtt gtaaaatcaa ccttttgggg ctgtcccaat
     5341 tcgccggcca tcatcggcat aaactcgccc ataaccgccg gagcagtctt gggatcggtt
     5401 tttttgtcaa catcttctac ttcatagccg ataatttggc cgggatacac atcaaagtca
     5461 aaatcccgcc attgtcctaa ataatatttg tccacttcca ccttgacccc ttcgactgct
     5521 ttggaagcat ctgctttaag gataacttcc atcgggaaaa cataaacccg cttgggaaca
     5581 tatacttcag gcgtaggatc tgtgtaggaa ctccatagga cagtttggtt tttaaaagcc
     5641 acctgatcgc tttggaacca atccttgccg gcgatgggat taaatactcc cagacgaatg
     5701 cggtactgat aggttttacc gggcactgcc gtatcatcat gcgcccagac cagaagcgga
     5761 tctttacgga tttcgatatt gtctttttcc ttcaggattt ctttctgcat atcgttcttg
     5821 acatccgtta ttgtccgttc cttgcggact tcaggacgca gtagagcagg attttcgaaa
     5881 cccggtccca tcagcagctc atcttgtcct cccctgcggg cagtgccggg acgcgtttgc
     5941 tgctgctgcg gacgctgcgg ctgaacgggc tgtcggcgct gcggggttgg ccgggttggg
     6001 tccgttacat tgggagtcgt acgctgacgg ctctgttctt cgcggcgctt gcgttcctgc
     6061 gcctgctctt cttttttcag aatatcaaag gcttccaaca ggaatttagg cggcatccat
     6121 tcggtacggc tgatagtgaa tgtataggct tcaggctgca ggatatcaaa ctgaacattg
     6181 gtattttcaa actgggaccg ccagatgccg actccaagct gggcctgttc gagagtcatc
     6241 ggcagctctt ctaaaagctt tttgtaggca tcgattttgg cacggggtac cgtctcccaa
     6301 tctccccacg ttccatcgcc ctgcttgacg cgccgctgca gctcgagttt ggcaaagacc
     6361 ggtttggcca aaacaggatc ctgccacgag gatttaagcc gcggcccttt aaaactctgc
     6421 ataaaattat tataaaggtt ctggacattg agcttggccg agacggttat caggtctata
     6481 tctcccagct cacaaacaac ctgcttatac ggccggtcag gaccaatctc ttccgtcggc
     6541 agctgggcag cgcctcgcaa agcagctacc gccacatcgg tcagaggcgg tatctgcggc
     6601 agtgcataca tccgctcttc ttcaattaca atatctccgg ctccgggata aggcacagaa
     6661 agagtagaaa gctgcggcac agcacagcgc acatactggt caaaatcggc cagataagtc
     6721 ttgtcatata ccacgggaac agccggctgc tccatcgccg gcagcgcctg ttcaaccttc
     6781 tgcttgatat agcggtctat atcgcccggg cctaaacggc ggcgatcaat ctcaacgctg
     6841 tagggacttc cgatgacata gacccacagc agaaacaaac taatcagcgc aatcacgccc
     6901 agcacgatct tatcaatctg attttcaaga atccgcagaa tatccttgct tttcattgtt
     6961 ctatttcctg tgttagcgca gctgcttatt tgttcaattg ccccatgcta catttcaaag
     7021 cccaatccgc cggccggggc tgccggcgaa gaagcagccg ggcctgatcc cggcatcccg
     7081 gaggccgttt ccagccgttt tttgacgggt gccggcttga tcgcatcata ccctttccga
     7141 ttaaactgat actcgcagac aaggtccagc ttcatcacgg cccctttgcc atagcggtaa
     7201 agttcgtgtt cagcggcgga tttgtcgatg actctgacat cgctttgaag aattgtaatc
     7261 tgattatgac gggattcaac cagctgaccg tctttggtaa aatcggttcg gaatgtatgc
     7321 ggcttttcgc tgcacagctc cttcataaac gccagcacaa aacggttatc caccagtacc
     7381 gaaacggcaa aatgaataat atccatctcc tcattgcaga gccgtccggt aggcgaagcc
     7441 gccacaaaga tgcccggctg ggccgctgtg acatactggg gaatgtcccg catgccggcg
     7501 gtataaccgt aatattcact ggccgcatac gaggaagaca cacccgctgc tacaaccgga
     7561 ccggaaaaac tcactcccat tagtcgcttc acaggcgcat ttgtcacctg tgcagatgtg
     7621 ccgttaagtt ttacaatcgt atcaacaata tcttcataaa tccaaaaggc aacctgcgaa
     7681 tcccagcaat cctgcagggc ttggtctttg gatgtatagc tgtacttctc ccagaaatca
     7741 taccaagtaa atgctttggg gtgtgcatag acggaaatct cctgcgcccg cgtctgacac
     7801 aaggcatcca ccatcggatc ctgggcagta ccgggtcttc gcaccggact gtacgcttct
     7861 cctatcgggc gggctgccgc accggttcgg gcacggattt ccgcatcgct gggggcatcc
     7921 agagcatgca ttcgtcttat cagatcttct atccggctgc ggtaacgctg gccgaaccct
     7981 atatagattt gcgaggatgg atccgtcggc tccggaaaaa tgacataatc gtatgtaatc
     8041 aaatccctct ggctgctctg aacagccaag gttcgtatgc cggcggcttc ctcttccagc
     8101 ttgtccatat agcgtttgac ttcggccggc tgatctttcg atggaacctg actgacagca
     8161 gactgaacgg aacgagccgt tcggacgctg ttttccattc tccctttgac tttattgccc
     8221 agccacagag tgggaataaa catcatcagt gccgcaatag caataatcac cgaaggcagc
     8281 agcgaggtgt actttttcag cgtaccagaa accggtttga gaaatcccat cgatatattc
     8341 atagcaaaaa acctgtctta aatcctgtat tgttgcgtat ttcctacatt cctatcatag
     8401 cgccggctgc agtgccttcc ggtttgggcg gggcttcttt ccagacaaac tttgcctgta
     8461 tccggaacca gtaatcccgc tcaatatact gcggggcacc ggatatggga ttaaatttct
     8521 tcttgcccag atctttgtca gtcagccggg gatccgcact gatatccttt tgcgtaatga
     8581 tatcgaaggt cttgctgatt tcttccccgg tcatcgggtc aaccaatacg gtttcagtat
     8641 acacatagtc ggctccccgc ccgcctactc cgtaatcgcc gatatagacc ggcatcgctg
     8701 cgactccggt cggtgacgtt ggtttcggaa cacgttcgac ttccttgata atgccgatgc
     8761 ccggcggaac cgtctttgaa tccaaatcaa ccaatcccgt ttctacttta aaatgagcca
     8821 catcgtgcct gtcaaacagc tcgaacggaa tacccggaaa caatttggcc agattttcaa
     8881 atcgggtaac aacaccccag cggctttggt cctctccgac actgaccgga tcgagcagtt
     8941 cggatatctt gcgatagggg ctgtagccct cgatgagcag ggtaaagccc atgcctgttt
     9001 tttccgtttg ggtccccggc ataggggcac cgggaattcc catatctccg ccgggcataa
     9061 caggcggctg catcatttcg agcatcggat caatcaaaat aggcatcgtc ggagtacgtc
     9121 ggcgcggtgt catcgttcgg tcctgtgtgc ccggctgcgg aaatttggct ttggccagat
     9181 catcggaata ttccattgcg atacgggtta taaacagcag cttgcgctcg ggccttggca
     9241 ctgcaagaac accttccaca ttgccgctct cgacagcttc atagaggtct ttctgttcgg
     9301 gcgtgttctc tttattgggc aggcatttaa ccagggtttc tacgaattgc ggaacggcct
     9361 gacgatactt gaaggactcc atatatttct tgatttcgtc ctccaaaggc tgaagccggc
     9421 tttgctgctc ggcaatactg ccggtgatgg cattgatctg accgagaatg gctgccgctt
     9481 cattgcgcgt ggcctgatgg ctgtcgtaag ttttcaggtc tttataggca cgtccaagac
     9541 ccagcaccat caccgccagc agaacactgg ctgccgccgt aaagatgcgg gcttttcgcg
     9601 tccacgccat agcccgggca attttgcggg gcagcagatt ggtccggatt ttggcttcgc
     9661 ccagcagctg aacgcccaat ccgtatgcaa tgccgaaatc agaaacattc tcgtgaaact
     9721 ttgcaaccga aatatccggc gacacggcta accgttcgaa tgtgtcgggc ttgataacct
     9781 gcatgccgag agtttgagtc agatatttcg acagcccctg cagcttcatc ccgccgccca
     9841 tggcaatgac tttgctgaat gtcttatccc ggccttcggg gctgctgctg taaaagccca
     9901 gcgaccgctg aatttcgctg cccagatcgg tataaacgga tttcattgct gtataaatct
     9961 gccgcatgta tttgctcatc ggcgctgtcc gcttgagctt ttcggcggtt tcaaagtttt
    10021 tatgaaacgc gtcggcaatg gctcgggtga aagtgtttcc gccgatgcgg atgctccgct
    10081 gccaaattgt gtccctcgag cagacggcca gtgtggtatt ttctgccccc acatcaagaa
    10141 tgatcaccgc tttctgcgga aactgctcca gctcctttaa gtcatacaca gcataattgt
    10201 acaaggccat cggggcaatc tggacgcagg ttacacggag cttttccttg gtaaaatggt
    10261 ccattacttc ggctatcagc tcatttttta tggcaaacag accaacggat ttatcaggac
    10321 tgtcttcggc ttccatcagc tgccaatccc attcgacctc gttgatatcg aagggaatct
    10381 gctggacagc ttcgaattgg acaatttcag ggattttttt gggttcaaca ggaggcaatt
    10441 taacaaagcg ggcaaaacta ttttggccgg caatcgagat agccacctcg cttttggaca
    10501 aatccttttc atcgagcagc ttccgcaggg tctcggcaat aatctggtct ttgtcttcct
    10561 gctgaaggtc acccgaagaa aggtttttcg aatgttcgat gtatgcaaaa tcggtcactt
    10621 ccagcccctg atcgccatga cgcatctgga gcgccttgag ggcattggtt ccaacatcga
    10681 ttgcccacac accttgtgcc gctgcggcca taactcaatc tcccaagata tggaataatt
    10741 acctgcattc acaatacgct ggtttgctct tttctgtctg gactgttttt cgcccttttc
    10801 cggaaattcc agaccagcaa cagagccaaa accctctttc agagtgagac aacattagat
    10861 tcattaccgc ccacatctgt aaaatcaggt ttaatagtgt aatataacac aggaattacc
    10921 tgattaacaa ccgaaaaata aggtaaaatc cgcatattcg ggactgtctg tctttttata
    10981 cccgacaaga aacatcgagg tttgtaacaa caatgtaaaa aaacaatttt accggaaaaa
    11041 ttgtgtctca gttttgtttt gcctgtcagg ttttcatcga ttgacagtat caagatttgt
    11101 cagccagttt atccatatat gcctgtaaag gtcgatattc tcgataaaac cctcattgtg
    11161 tgatccctta agcatcccaa accacttcgg ttcgttggcg gcatcgtaaa tcgtttttcc
    11221 gaagcgaaac ggaactatct catcatccgg gctgtgaata accagaatgg ggcaatggag
    11281 ctgtctgaca gctgtaaggg tgtcatatct gaaacggctg aagagccgca caggcagcca
    11341 aggataataa tgagagccga tatcgggaat tgaagtaaac gtgctttcta aaaccaatcc
    11401 agccggattg acatcctttg ccgtcaaagc ggcgatgcat ccccccaaag agcgtccaaa
    11461 cagaataatc ctgcgggcgg ggatgccttt ttttatcagc caatcccatc cggcacggat
    11521 gtcttgaagc gttccctttt cggtaggttt gccggtactt tgaccatagc cgcgataatc
    11581 cacaatcagt acggccagtc ctaactgatg aaacaatttg agggtatcga gccgatggga
    11641 gatattccct gcattgccat ggcaaaacag tatagtccag cattcattgg cattatggcc
    11701 ggccgggata aaccacccag ccagacgctg cccatcggaa gtgttcagtg agatcgattc
    11761 atagacaagc cccgcatctg ccggcgtatt gtcataagta cggctgggct gatataccat
    11821 ggccggctgg aaaagataca gaagtaagcc gacaccgccg taaacggcgg cagcaataac
    11881 tgctattgcc aaaagaacac ccataaatcc gcctttttta taacgaaggt aacagaccgc
    11941 cgctgttaag acagcagcca atatccacat aatccatcgc atcctgtctc cattcttttt
    12001 ggtgttgccg caattgagaa aacgtataaa gttgatacgg tgtcagctgc tttgaatacg
    12061 gcattttcac ttgttttctt cttaaaaacc gcttattatt ttaacatatg gaagtttata
    12121 aggatatgat tctccttaca atataaaagg aaattgtatg tctaaactcg ccttacttat
    12181 tctcactgtc ttttctctgg tttcctgtac cgctttatcg gccgcttccg gcaaaaaaac
    12241 cggttccccc cttgacaatc tgccgcctta tatgcgccag attacgcact tcggcgagcg
    12301 agccgactgg tccggcgacg ggaaaaaaat tctcttttta gaacgaactt tcggcgatgt
    12361 ctttgaggtt gagccggcca caggaatcat ccggccggta acacatcact ttttccatga
    12421 gggtttcacc cgggcgctat atctggccaa cggtgatatt ctgctgtctg gctcacgcac
    12481 atttgatgct gccgatccat gggccagccg cagcgaaaaa caagcggaat tatgggtgct
    12541 cagcaaagac ttgaatgccc ccgcggtgcc gctgggggtg cattgctcgg aaggtccggc
    12601 cgtatcacga aaacggatgt atatcgcctg gacacaaggg aatgcattct ggggggctga
    12661 tatcgtttat gaaaatggca aacccaaact ttccggcaag acaaaactgc tggataaaaa
    12721 agacctgccc tttgagtgcg gactggaaac ccagaacttt cggccgcctg ccgaggagga
    12781 attgatattt tctgcctatg actatcaagg cacagaagtc tgcggtctga acctcaaaac
    12841 gggaaaaatc accaattatt cgaatgctcc aaatcagtat gacgaacctg aaggcatttt
    12901 ccccgatggg caatacacgc ttgtcgaatg caacaagcat atggatattg cagagggcat
    12961 tcaggaaatt gatatttaca aactcgccct cgacggctcc ggcagaatgg agcgcataac
    13021 atattttgct gactacatcg gctacaaagc cacaaatccg gtcgtcagtg atgatgggcg
    13081 ttttattgcg tttcaattcg ctaaaaaagg cgatccggcc ggagtcggcc gcggcattct
    13141 ggtccttgac atggccgctt ttgagaaatc gcttcaggag agaaaataaa ctgtttgaat
    13201 ttcacaaaac ctgaatgtta cagccgtcgc ttaaagcgga gaaaccgcct gcttgcatcg
    13261 ctgctttgaa ttctacagta ttctgcagtt catcggtgtc atttatcagc gcaataaaaa
    13321 agggaggccg gcgcgtttta tcaaaaatag gttgtgggcc gcactccctg ttgccgctgt
    13381 gccgttagtg caccttaccg cggatggtac gaggcgatgt tcgccagtat gcaccggcaa
    13441 catctaaatc ttacaaaaaa aagacggctg cgtcaagaaa aaactgaaat gtgaagttca
    13501 attactccgc tttaaccaga atatccatat atttgacgcc ccatttacgg gcctgctcat
    13561 gcgagctgaa gaagacgtcg atgcggtttc ctttaatcgc cctgccgcga tccattacct
    13621 tcacgggctg acctgcatta tagccgggga taatcacttc tgttccaaac ggaaacttct
    13681 tatcggcagc aacaaaaaca tctccctgcc ggatgcggtg gctgcaagcg gtccggccgt
    13741 ctgaatattg accgcagcat ttgctgcacg gacaatagcc tgttactcgc atccgcatca
    13801 cctgccagtg ttcggtctct tgcggttttt cataagcagg ggaaggttcg gctgttataa
    13861 gcatagcaag cagctcattg tcgctgattt gcgaaggctc atccggcaga atagactgtt
    13921 ctatatcttc agtatcagga tccgtctgag gaagagttgt ctggagataa accacggaat
    13981 caccttccag cacctgcggt atcacccacg ctgccggcag tatgccggca agaagcaatc
    14041 ccgccgccag tgccgtcatc aatttactgc cgatgtaaaa acgcccttga ttctgtgcca
    14101 aaacagcacc tttccaaatg aacttctctt acagacgatc tctgcaggca atcgcctctt
    14161 ttgccaaaac tcaacgccct ctcctgcgtt ttcagcaaaa ccgctctggt aaaattgaaa
    14221 aggccatccc tggcaaattg attaaattat gcagaacaaa ggtgttttaa gcatttactg
    14281 ctgtgggatt aaagatacgg agtatagtag aaaaagtcaa gatttttttg tatcaaatat
    14341 cacaaaaacg cccgataaaa aacaacttag aagacataat aagacggcct attaaaaaaa
    14401 atttaaaact tcaggatata tggactctat aaatagagtc cctttttgct gatcccccat
    14461 ttcaaccgtc aggaattacc cctctcccat ccaaaacgag ccgcgggctt ccatgcccac
    14521 agctcatata ccgcctgata aaaattatct tgtttgcaga cgcggattct tcgtacacaa
    14581 actaccctga ctatagatgt aatcgaaatg caatctgccg gttagcatcc acccagcatc
    14641 caattgattc gcattatatt ataacgctgc atttggagcg tgcaatcatt ttcagcggtt
    14701 ttttaaaaaa tcaacggttt gcctttcctt tgccgccgcg gcagaacaag aaaacaacaa
    14761 cagccgtgat aacaaccaca acaagcactt cactagggct taacaacaca gctgtcataa
    14821 catcctttct tttttgaatg aatccttatc gcaagacata gaagctatcc agaagtctgc
    14881 gaagatgctt cggctggctg ttcctgtttg gctttcagat tgaccggcag gtccagagaa
    14941 aatgtcgaac cctttcccac ttcactttcc aaatgaatgg atgccgccag caaatcggca
    15001 agctgcctgc agatggctaa tcccaagccc gttccctcgc cctttcgggt aatcgaacca
    15061 tccagctgat gaaacttttc aaagatatac gtctggtcct gcggagagat gccggggcct
    15121 gtgtcggata ttgaaatgcg gacaatggcc gtttcgggca tcgcggcgga aatacgaaca
    15181 cggcctcctt caggggtaaa tttaatcgca ttgctcagga ggttatagag aatctgctga
    15241 accttgtgcg gatcagtctg aaccagcggc agttgcggat ctatttccaa gtccacctgt
    15301 attttcttct gctcgctcaa cggagaaaaa aatgccgtta atgtttctaa aagctgaggg
    15361 atgctggttt tttcgatatg caggctcatc ttgccggctt cggctttggc caagtccagc
    15421 aggtcattga tcatattcag cagagaccgt ccgctggaaa tgatattctc ggcccagcgg
    15481 cggaccttct cctcttcggc ctgcggcttt tcccgaatca gctcggcaaa tccaagaatt
    15541 gcattaagcg gcgttctgaa ttcatgggac atattggcca aaaattcact tttgagcttg
    15601 ttggccttaa acagctcgat attcttttcg gaaagctggc tgattttggc atccagctgc
    15661 tgattggctt tttcgagttt gtgctgcgac tccatcaggt tatccagcat atggttgaaa
    15721 gcatccgaaa gccgttcata ttcatcgccg gttttgatgg cgcttcgaat gtcgtaattg
    15781 ccttcggcta tattattgac caaagcgcga agctggcgta tcggactgag aatgaccctc
    15841 tgggtaatag cataaaaagc caccattgcc cctgtagctg ccagaagtcc cgctactaca
    15901 acccacaggc gattcataaa cagcgtccaa gccagacctt gggctgttgt ccgggcgatt
    15961 aacagcccga tttcctgatt tttgctgaat accggtgccg ggccctgttc aaaatggcac
    16021 cgcaggcagt tttcgtcagc cagcacaaga cgcagataat gatttttgaa tcccccctct
    16081 gccgttcgtt cggtccaggc gatatcactg atggaagcat ctttcagcag cagctcaagc
    16141 cgctggcgtt cggattcgct ggcctcttcg gcaggaatcc ggccttcgtt gtcaaatcgg
    16201 agccatcgaa taaccggctg agcaccggta atggcctcac cgacttcgga aagacgaggc
    16261 agctgctgcg ggtccaatgc tgtccatcga aaatgatttt catagacggc ctgagtgata
    16321 gcccggcctg catcaagagc cgatttttca gtcagtttgt tcatccagaa atagggaatc
    16381 agcagtgcca aaacaaggct gaaaagcaca gccccgccaa actgcaggcg gcatttctcc
    16441 gcaagcgtaa gaggcttgcg gtacaccgcg gtccgcaacc gcaggcagta tcgcttgagt
    16501 tcggccagca tatttatccc cgattatgcc gaaacaggca gtcaattaca tttgaccggt
    16561 ttctacgctt ttctgcccga ccgtccgttc agctttcctt gtccgggtcg ctttctacct
    16621 gatgcagatg tttttgcacg cgcagattca gttcatccag ctgtgattgt tcaacactcg
    16681 acggcgcatc ggtcaataaa cactgtccgc gctgcgtctt ggggaatgca ataacatcac
    16741 gaatattctc cgtaccagtc agcagcatcg tcagacggtc cagaccaaat gcaatgcccc
    16801 catggggcgg agcgccgtat tcgagcgcct tgaggaagaa accaaaacgg tcctgtgcct
    16861 gctttttaga gatattcagt aaatcaaata cctttgcctg cacagccgga ttgtgaatac
    16921 gcacagaccc cccgccgatt tccgaaccgt tgaccaccaa gtcataggcc tgtgaacgga
    16981 tatgggcggg atccgtatcc agcttgccca aatcctcttc caccggcgag gtaaacggat
    17041 gatgcaggga atcataccgc ttttcgtctt cattccattc aaacaagggg aagtccacca
    17101 cccagacaaa tttgaaatcg tccggcttga acaagcccag ttcacgtccc agcttgaccc
    17161 gcagcggcgc cagtgctttg ttggctgcat tttccgcatc tgccacaaac agcatcaaat
    17221 cgccttcctt ggcatcaaac cgccggacca gctccagcag ctggtcttgc gtgaaaaact
    17281 tgctgatgct gctcttgggc agaacttttc cgctgtcatc cttttccact ttcatccagg
    17341 caagaccttt ggccccgaaa tcgctgacaa aggcggtaag ggttttttcg atgtcgctgc
    17401 ggctgtattt ggaaccgccg ggagcacaca gccccttgac aacgccgccc ttttgaacag
    17461 tcgaaacaaa aacctgaaac gtgctttggg ctgcgatatc cgagatattc tgcagcttca
    17521 tctcaaaccg catatccggc cggtcaatcc cgtactcctc catcgcctgc tgatacgtta
    17581 tccgcggcac aggattgggc aaatccacat tgataatctc cttaaagatt cgacggatca
    17641 ggccttcgtt catccgcatc acatcatcgg aatcgacaaa actcatctcc acatcaatct
    17701 gggtgaactc tgcctgccgg tcagcacgcg gatcctcatc gcgaaaacac cgcacaatct
    17761 gaaaataccg gtcggtgccg gcgaccatca atatctgctt aaacagctga ggcgattgag
    17821 gcagagcata gaaggctccg cggaccagcc ggctgggcac cagaaaatcg cgggcacctt
    17881 cgggcgtgct tttgccgagc atcggagttt caatctccca gaagccgttc tcgtcaaagt
    17941 aatcccgagc aatcttggta atacgatgcc gcacagccag tttttcctgc atggccgaac
    18001 ggcgcaaatc gatataacgg tattgcaggc ggatttcttc actgacttgg ttggcggcat
    18061 caatctcaaa cggtggggtt ttggcggtat taagaatttc caactgacaa gcgagcactt
    18121 caatctggcc ggtggccaat ttgggatttt ccaatccctg accgcgggga cgcactttgc
    18181 cggcaaccgt gataacccac tcgcatcgca gcgtgcgggc aagccggtgc atttgggcat
    18241 cctgctcctg atcaaataca atctgagtca gaccggtttt gtcacgtaaa tcgataaaaa
    18301 caagactgcc atggtctcga tacgaattga cccagccgct caaaatcacg gcctgaccgt
    18361 catctgttaa ccgcagctgt ccgcaaaggt gcgttcgttt cagcatatca tctccactta
    18421 tacagtattt tccggctcgg catacatcct tttgacaccc gtttcgttaa caacaactaa
    18481 atatcgataa aggaggtaca atacagaaaa agcccgcctg cgtcaataag aatcccgcag
    18541 acaggcctgt gctgattttt gacttttttg tggccggcac agtatattcg cacgacagct
    18601 gtggcttttt ttatcatgca agttattgca tgacaagaca ttgccgccac tcagcccagc
    18661 agttcatcta tggcttcggt tatatcatct ttggaagtca gcccaaccca ttttttcgca
    18721 acttgaccgt tttggaaaag tatcagcgtg ggaatggcgc tgatggcgtg ctgaatagct
    18781 gcctgacggt gctcatccgt atttaccttt ccgattgttg ctttaccagc atattcagcg
    18841 gctatttcct caataacagg ggcaatcatt cggcaaggac cgcaccaagg cgcccagaaa
    18901 tcaaccagca ccaccccatt gccattgacg actgtatcga atgtatcgtc cgttaattca
    18961 accacattgc cggccataaa taatcctttc gtgtcaggta tttgggtttt tgtttcatcc
    19021 gacggcattc tatcggcggc ttgtccactt gtcaaatacc gtttttcatt ttgattgtat
    19081 tgcctggaag agaagagctt ttacttgctc aaatcatact taatggagta agaatcgccg
    19141 tcaaattcgg tattaatttt gtagccggaa ttgtaaaaga tggtcagcat cgctttattg
    19201 gtcggcaaaa ccgttgcata gaaggacttg acgccgcggt ctttggcaat gcgggtcaga
    19261 taatccagca gcatggcccc cattccttta tcctgccatt cgtcctgcac aataaacgcc
    19321 acttcggcga cctgcgtctt aggatcgaga aaatactggg caatagcgac gatttcctcg
    19381 gcgccttccg ggccggggac aactcccaca attgccagat tctgttcata atcaatattg
    19441 gttattttct gcacatcctt gtgggggaat gttttggtat gcgtaaaata ccgcttctta
    19501 accgactcgg aagataggga atagagcata tcggccaaag ccgcctcatc cgtaggcttg
    19561 acaggcctga aaaatatttc tgtgccgtcc ttgagagtaa caaagcgttc cagttcctga
    19621 ggatagtgaa ctttgtccca ttcgagttca atttggtccg gctgcaggta gcgctgggct
    19681 ttggctgcct gaatcagttc tttgcggaat ttcggatggg caatattgat cagcgacaaa
    19741 acccgctgac gaatcgactt gccgtgcagg taggctatgc cgtattcggt aacgacataa
    19801 tgcacatcac cgcgggttgt taccacaccg gctccatcgg ccagcttggg aacaattcgc
    19861 gacacagtat cgtctttggc ggttgaaggc atcgcaataa tgggtttacc gccgcgcgaa
    19921 cgggcacagc cgcggatgaa atcgacctgc cccccgatgc cgctgtaaaa gcggtagccc
    19981 agcgaatcgg cacagacctg accggtcaaa tctatctcca gaccgacatt gatgccgacc
    20041 attttgtcat gctgggcgat aatgaacgga tcattaacat actcggtcgg atgaaattca
    20101 aaaaacggat tgttgtcgat atattcatag agcttctgcg agcccatgca aaagctggca
    20161 acaactttgc cgttgttgat ggtcttttta gaacaggtta ccgcaccgca ttcaatcagc
    20221 tcgataatcc aatcaccgaa catctcggta tgaacgccta aatccttctt gtcgtgaagg
    20281 aattcggcca tggcctgcgg gatgcggcca atcccgcact caatcgtgct gccgtcttcc
    20341 accagccggg ccagatttcg tccaatgcgg cgaagcacat cgttgggggg atccatcaca
    20401 acagtaatga tgggctcgtc atacggcacc atggcatcga tgttgttgac gtgaataaag
    20461 ctgttgccca tggtgcgggg catctgctgg ttaacttgcg caataacata tttagcattg
    20521 tccgctgcag acttgacaat gtccactgac acccccagac tgcacagccc gtttacatcc
    20581 ggcggggtaa cagaaatcag cgccacatcc aaaggaatac ggccgctttc aaactgccgg
    20641 ggaatatcgg acagaaaaat tggggtataa tcgccgatac cctgctcgaa tgcgccgcgg
    20701 acattctcgg caataaagaa actgttcatc ttgaattttt cacgatactt ttcatcggca
    20761 taaggagcat cgcccatcgt cagcagatga atgatgtggg catcgtagat atggctgccg
    20821 tattccacca gcgcccgaac cagatgctgg ggctgtccgc agccggtgcc gataaagata
    20881 ctgtcccccg gcttaatcag tttcatcgct tcttcggcgg tttgaatcaa atggctgtat
    20941 ttttgcttcc aatcaaacac actcatactt ccatccttcc cgcacgatat tgtgggtttt
    21001 tggttctgtc aggccgatct tatcgctgtt ccagcgtcat catcgcatca accgcaatag
    21061 gcgttgttcc ttctctgccg acgacatacg gattgatgtc aatttcctta atctgaggga
    21121 attcggttgc aagctgagaa agccgctgaa gcccctcggc aatggctcca atatcaacac
    21181 cttcttggcc gcgcgttcct ttgagaacat catacgtttt cgtacgcacc agcatttggc
    21241 gggcttcttc ggccgtcagc ggagccggat aaaaaaccac atcctccagc acttccacca
    21301 gccgtccgcc catgccgaac atcatcagcg gcccaaaacg gggatcccgg ttcatcccta
    21361 aaataacctc acggccccca gtgcacattt tttcaacata aatacccaat atcttggcct
    21421 gcggcacttt cctgggtatg cggtacatca tcaaatcaaa tacatcaaga acttcctgat
    21481 cgctgttaat attggttttt acgccgccga cgtcggcttt gtgcaggata tccggcgacc
    21541 agaccttcat cacaaccgga tagccgattt gattggcaat attggcggcc tgttcgggcg
    21601 tcgccgcaat gcccccttcg ggcgtaacaa aaccataggc ctccagaatt tccttggctt
    21661 ccatctcgcc cacctctgcc tgctgcaggc gaatatgccg ctctataatc cgttcgacct
    21721 tgcggcgatt aacattaaac agcttgacaa cacgcggggg gcgtgacttc cactcggcat
    21781 agcgcgacat cgttttaaca gcacgcaccg cgctttcagg ggaatcatac ggaggtattt
    21841 tgccgtcccg caggacgcga accgcctcgg caactcggcc ggcaccgaga aaacaagcca
    21901 gaatgggctt gttgcggccg ttctgattgg caacacgcac taccgcatga gccgtctgtg
    21961 tgcattcggt catcgcatgg gggctgagca ataccagcgc tgtatctaca ttgggatctt
    22021 ccagcactgc ctgcagcgca aactcgtagc ggtccgaaag agcatcacca agcaaatcaa
    22081 taggattatt aatgcttgct gcccgcgaca gttcttgtga ttgcgaaagt cgtttgcggg
    22141 tttcttcgga aaactgggcc aattgcaggc cttcccgctc gatggcatcc gttgccatga
    22201 tgccagctcc gccggcgttg gcgataacag ctacccgcga gcctttcggc agcggctggt
    22261 aggcaaatgc tctggcgtaa tcaaactggt ccttaatcga ttcacaccga ataactcctg
    22321 cccgttcaaa aacgcattcg tacgcgcttt cggtttcggc cagccgtcct gtatgagacg
    22381 aggccgcacg ggcacctgcg gccgtaaaac cggctttgag cagcagaatc ggcttctggc
    22441 ggctgatgct ttcggcctgc cgtataaaag catcgccatc agcaatcgtt tccagatagc
    22501 cggcaatcac ttccgtctcg gcatcttcgc ccagagcacg gataatggtc agttcatcca
    22561 catcggcctt attgccgata ctgataagtt tgctgaatcc cagatgggta tcccgcgcca
    22621 tatcaacaat tgccgccaac agcgagcccg attgggaaaa ataaccgatt gagccgggct
    22681 ggggcagatc gccgccgaaa ctgacattca atttgcgaga tggcaccata agcccgatac
    22741 agttggggcc aataatgcga aggccggatg tttttgcgat gcgcactatc gacttttcaa
    22801 gttccgcccc ttcaggccca gcttccttga aaccggatga aataatgata actgctttaa
    22861 cgcccaagtt ggcacaatcc tgaactgcct gcgggacaag cttagccgac acaaccaata
    22921 caaccaaatc cggtacctgt ccaatggatt tcaaatccgg atatgccttc agaccctgaa
    22981 tctccggtgc tgttggatta acaggaaaga ttttgccggg atagcccccc ttcagcagac
    23041 cggcaagaat ctcataacca accttgctcg gctgacggga tgcgcccaca atcgccacgg
    23101 attgaggatt aaaaaaacga tccagcgtca taggttcctc ttagtcgtct tgtgcgagtc
    23161 ggcagcaata caaaattcct tgccgcatct atcatccatc ctcttttata gcgttttgac
    23221 aggcaattgt aaaggatttt tgtccctttt gtgtcgttat ttggaacaaa aatcccccga
    23281 aagggtgaaa aaacagaaaa tactttacat aaaacgggat tgtcagtata gtacagccga
    23341 cgtgccgagg tagctcaacg gtagagcaca gctttcgtaa agctggggtt gtgggttcaa
    23401 atcccatcct cggctgtttc ttttggcctg aaaacaggaa gatttaatcc cctttaccgc
    23461 cgttttcttt ccacattgcc agcggtgcgg ctataaacca aacaaacgtt gcccacagca
    23521 ttacgttttt ggctgtttca agtgttatcg catttgccaa aaaaagcaca gccggagcta
    23581 ccagcacaat cagggaaatc caagccgtta actttgcaca taatcgcata ttccagttcc
    23641 tcaggccgga tttttctttt gataaataaa acttaaaacg atatataaaa ccgctgtcat
    23701 aaaccatccc ggcaatgaaa cgaaataaat ctgcatccgg ccggaaagaa caagccagcc
    23761 gcatacagcc agtgtaatga accacgccag acctgctgcc caattaaact gcgatccgga
    23821 aagccgggca taattgctcc gcagaccgag cttgtccatc agccagaaat cgacaaaaat
    23881 tacagctccc atcggcatca gcaccagagc gaatgtcgcg acaaaatcca gcagattcat
    23941 cgctacagca ggaaacatcc ccgagactgt tgccaccaga ccaattacca atgtaaccca
    24001 aaaaagaggt tttttcggca taataccctg aaatgccaaa cctgcacggt aaatagtcgg
    24061 attggcagtt gtccaagcag ctacaataac gcaaatcagg ccggtcaagc cggcagcccg
    24121 ataggtcatc ggtccgggca ggacatcggt atcggccgga ttcaggtgca gctgataggc
    24181 atacaaaatc gatgcacaaa tccaagccat aaaatggccg acatacattc ctgccgccga
    24241 tgcgacacca taccaggact tgcgggcata gcggaaaacc gacaaatcac tcattccgat
    24301 atgccacgcc atattgcaga accaggcaaa aaacataata tgccagaatg taaacttaac
    24361 ctgtcccggc aagggcggcc cccctttcca gatacgcatt tttgccagcg tccaaagatc
    24421 cgaataagat tgaatctcca tatcagcggc gtttagaaac tgccgaaaac caatgaggcc
    24481 aaaagccaga aagaccaaga ccatccacgg cgcggcaata ttggcaaact ttgcaaccgt
    24541 tttatacccg caggcggcaa caacagcaat ggtcgttcct gcggcagcca ccgccagaat
    24601 ccagccgatg ctgttgggat acagatcatc aagacccggc attggaaact taaaccagat
    24661 gccgaaggcc gtcgctgaaa ccgtgatcat tgcgccggca aggatggtaa acatcacgcc
    24721 gttggccaga ttgtacacca taaccagacg gcggccgcag attttctcaa gctggtagta
    24781 cagcgtcagc cgggcacggg ttgctatcgg agcgcacaaa aacatccagc tcaaaacagc
    24841 caatgcattg ccgaccagca gaccgacaat catatcaaaa gcactgatgc cggaagcaac
    24901 aaacagcggt ccaatcatca gttctgtacc tgcacaatgt tcgccggcgt acattccgac
    24961 aaaactctta aatcccaaaa gggcatgcgg aggcacagga gttcgctcat actccgcagc
    25021 aggatgaaaa ccgtcagaat gacaggattc ggtttgcggg ctattttgag ctgcaccttg
    25081 ggtctgtgca caggtgtttg ttgtatggtc ggcgttcacg gctgttttac ccatagatgg
    25141 aagatacctg ctgtgcattc ggaagatttc gactctggcc ggcggatttg ctctggccca
    25201 gatagccgct ccagccggca ataaccgctg aggcaatcag cagcacaagc ccagccgcca
    25261 aaagcccctg tgtttttctg ctggtccctt tccactcgcc cagcatcaaa cccagcagct
    25321 ggctgaagag aatctgactg gccatcagca ccgcccagcc gatataggcg gtagtcccca
    25381 tcatgggctc accggtttta aagcagatga attgcgagca ccaaatcgct ccggccagac
    25441 ccgccaacac aagattggca agcacaggag catctttctt gacataatct cctgccgtct
    25501 tgtttttgag atttaaaaag atgcaccaga cggcattgac aacaaaacct cccagcagca
    25561 ccaccaccag caccggcatg ccagcccagg cacgggatgt gtgcggctct gttgtcattg
    25621 ccagattctc aatttccggc ccgccctgaa gaccgaaact catcgccgaa ctcatcaggc
    25681 ctgaaaacag ggccgccaaa atgccctttt tgaagttaaa ctctgctacc gcttttttct
    25741 tttcttcctc ggacagctcg ctttctttgg acattccggc cattccgaca aagacaatgc
    25801 ccaccaccga aacgactgcc gccgccactg atacaagtcc cgacggcgtt ttaaacatcg
    25861 ccgatacggc atcagaaccc ttgagcatcg gcggaatgag cgtgccggca gccgaacaca
    25921 gaccgcagcc catcgccaac cccaatccga agcccaaata gcgaatcatc aaaccccatg
    25981 tcaagccgcc gactccccac gctgccccgc acaggaaaca tttgattatt tcgttccgcg
    26041 gagcgccggc caaaaccgaa agaacattgg gcgaggtcgt cagggccaaa atccacggca
    26101 caataaccag tccaaaaacc gcataaacca gccaatagct ttcccatgcc catttggtca
    26161 cttttttaaa cggcagataa aaaacggctc cggccagacc gccgagcata aaaatcacga
    26221 taccaacggc cggattggct gctgcaagca gagtcgtcat cgtgttctcc ttaacagacg
    26281 gtttttatta gcgttttgaa agaacatcgg cctcgtattt tttcacttca tccaaccacg
    26341 ccatgccgac aggcacattg cggctcatgc agtagtagtc ccagacagca gcccacggca
    26401 gggttttgag ctcctccagc atcgccagcc ggcgggtgta gtccatcttc agctcaattt
    26461 ttcggagggt ttctgtcggc tccagcagag ctatcaggag ggcttttatc atattgcgca
    26521 ttccaatggt ccaggccgct acacggttga tgctggcatc aaagaaatcc agtccgatat
    26581 gcaccttgtc cagcttgccg ctgcgaacca gctctttggc aatggcctgc aattcatcat
    26641 ccagaataac cacatggtcg ctgtcccagc ggacaggccg gctgacatgc agcagcagct
    26701 ggggacagaa catcattacc gaggaaattt tttcggaaat catctccgtc ggatgaaaat
    26761 gccctgcatc cagacaaaga agtttctgcc gcgaaacagc atagcccaga taaaactcgt
    26821 gcgaaccgac tgtgtagctt tcggcaccga taccgaaaag tttggattcc accgcatcca
    26881 gattgtgagc aggattcact ttgaccgcaa aaattttatc cagcgaatcc atcagtcgca
    26941 accgcggcga aacacggtca acagggacat ctttgtagcc gtcgggaatc cagaaattgg
    27001 tgaccgtggt gctgcccagc tgcctgccga tggcagcgcc gattttgcgg caggcgatgc
    27061 cgtgctcaat ccagaagttg cggataccct tatcgggatg acttagggta aatccatcgg
    27121 cagccttcgg atgtgaaaaa taggtgggat taaaatccat ccccagctta ttggccttgg
    27181 cccagtcaat ccagctctgg aagtggcggg gctgcatttc gttgcggtcg atatatttgc
    27241 cgccgttatc aatgtagcag gcatgcagat tcagccgatg ccggccggga atcagactga
    27301 atgccttttc aatatcggct cgaagctggt caatcgtgcg ggctttgccg ggatagttgc
    27361 cggtggcctg aatgccgccg ccggacagct ctgccccgac cgtttcgaat ccgccgacat
    27421 catcgccctg ccagcaatgc atcgaaatcg ggatggccgc cagtttcttg agaaccttgt
    27481 ctgtatcgat tccaatctgg gcatactgct ctttggctat gctgtatgct ttttcaatag
    27541 tctttttcgt cattggcatc tcctgtaaaa ggcgtttgtg ttggcactaa atccgggctt
    27601 ttttgagctt cataaaacgt tcaaacggcg cctcccatgc agccgtattt ttcggagcat
    27661 aggtatgcat ttcaaaggac tggcggatga tttttcttgc ttcggccagc gacgggatat
    27721 gccccagtcc gatcgcctgc atcgcaatat ttccgataac ggtggcttcc accgggccgg
    27781 taatgaccgt ggtaccggtg gcatccgccg ccagctgatt gagcagcgta ttctgtgtgc
    27841 cgccgccgac aatatgcagc ttttcaattc gggtaccgta aatctcgcaa agctgctgca
    27901 tcaccagccg atactgcagc gccagacttt ccagacagca tcggacataa gccccttccg
    27961 aagcaggaac aggctggccg gtcttgcggc agaatgcggc gatggcctcg gtcatcgaag
    28021 gcggattcat aaacatatcc cggtcggggt caattaagct ggcaaaggcc ggagcttgcg
    28081 cagccatctg ggtcagtgtg ccgtaatcca gctccttggc aaaggccgca cggcagcgct
    28141 ggaccagcca caatcccata atatttttga gaaaccgaat cgtatcaaac accccgccct
    28201 cattggtaaa gttgtactcg aaggccttgg gagatgaaac ggggctgtcg gcttcaatcc
    28261 ccatcagcga ccatgtacca gaactgatat aagcccagtt tttgccctct gccggcacag
    28321 ccgccacagc cgaagcagta tcgtgcgtgc cgggggcgat aaccggaatc ggccccagac
    28381 cggtaacagc ctgcagatgg ctgctgatag gcccgattgt ggtgccgggc ttgattaacg
    28441 gatgcagaat cgacggcgat acgccgatgg ccttgaaaat gtcttcatcc cagcttttgg
    28501 tcttgagatt aatgccctgt gaggtggtgg catcggtgta ctcgttggtg agtttgccgg
    28561 taaacagata atggaaataa tcgggtatca tcagaaaatg agcggcattt ttgagcgctt
    28621 cgcttttttt ctcagccaga gccagaagct gatagataga attaaacttc aaaaacgcaa
    28681 taccggtctt ttcataaatg gtttcagacg gaatccgctt gaaaagcgtt tccatcatcc
    28741 catccgttcg gctgtcgcga taggccacag gcaagcccat ctgggtttcg tccctggcca
    28801 gcagcacaaa atccaccccc cagctgtcca cgccgatgct ctcaatgggc acatccgaac
    28861 cggcacactt cttgaaggcc tccagcatcg actcatacag gcgaaccaga ttccagcagt
    28921 actggccgcc gatggggacc attccattgg gaaaacggct gatttcttcc agacgcagtt
    28981 tgccatcctg caggatgcct tttacgaccc ggccgctttc ggcccccaga tcaatggctg
    29041 cataggcatg tacttcggcc ataaagtcct ccattattgc ttggttaact gatttttacg
    29101 gtacgactca acttcccagt tttcaataaa ttgccgcgct gtgtcggtca tatagtccac
    29161 acctccgaag gcagccgcca actgcctgac cagccctcca tcctgtgcca gttccagcgc
    29221 cagacgggct gccttggggc ttgttgaaac tccaaaaacg gcctcatcaa agatcattac
    29281 ctgcggggtg tatccatatc gggattgaaa tgcttttatg gattctgcac tcgggcggcc
    29341 gacaaacgga taggatttgg catagacaat gtggtcgggc gtcagcggac ctgcagctgg
    29401 ttcgaaaaaa ccactggcag caatatgcac agattcatcc tgcacagccg cgctgataag
    29461 ctgtcgggca cggtctgtct gccctgctga tggcataggc cccatctgaa ggtcaacaga
    29521 tatgccggca gctgcatatt cggcttccag ccggcccatg acttgggcat ataaagagcg
    29581 aattgtctcg gcgttgtcag ccgcagcaaa aagaccgtga tttttaagaa aaatcaaggc
    29641 cggctctctg ccgtacgcct gccggtatgt ctgaatctcc cggcggaccc gcatgcagag
    29701 cgtatagccc ggatcgatat aatccagcca caaggcatcg gggaagagct gacggcagac
    29761 aacagagccg ttttttgagc aggtcattcc gttgacaagg gacggatgcg tgtgtacaac
    29821 aaaacgtgct tctaaggcat tgtggagagg ggcctcgaca gaagcccggc cggagatgcc
    29881 gggcaagacg gctttttcca ttttttcctt aaccagcgcc tcgcgtgcgg ccgaatccgg
    29941 cgggggttct attgtataca attcagacag cttatgcctg tccagggcga taaagtcgcc
    30001 gggcttcaga tctgccagtg tcgttccgga cggcttgacc cagagcgtgt gcgcatcttt
    30061 gcaggaggta ttgccgccgc caccgcaaac ataacgccgc gtgccgaact catgcgataa
    30121 taccgtaatc gtgtcaagga tgtccaatcc gagtcctttc agaaaatcaa gcaaccttca
    30181 ttcttataac agcaagtccg gcaatttaca actctttctc ccccgcccca gcagcaatac
    30241 aatgctctac ttgacgaaac gaatctgccg gataagagtc aatgtatcct gcaccttgcg
    30301 tgttatgccg gcgaaatcac cagccgccac aagctctttg gtaatcagtt tggagcccat
    30361 ccccgcacag gcaataccgg catcaaacca ctcttttaag ctttccggcg ttggggaaat
    30421 gcccccggtc ggcatcagtt cagcccaggg gcaaggccct ttaacggctt tggcaaaagc
    30481 cgctccgccg acctgaccgc ccgggaaaat cttgcagatt tccacaccca attcgtgggc
    30541 tttgtgaatt tcggtaaccg aaccgcagcc cgggcagtac ggtattttac gtttattgca
    30601 cagcttggct gtttcctcat ccaaaagcgg cccgactata aaatctgcac ccgcagcaat
    30661 atacatcgcc gccgtcgggg catcgcagat agagccgact cccagaatca cctccggccg
    30721 cttactgtcg cggtattcgg tcagtttatt aaaaacattg atggcccggt cgccccgatt
    30781 ggtaaactcc accacccgcg ccccgccttc ggcacacgca tcaatgattc tgcaggcagt
    30841 ttcagaattg ggattgtaaa aaaccggaac aagtccgatt tccttcataa ctgtcaaggt
    30901 ttgcagtctg ctgtaacgcg ccatttttga tgcctttcga attcttctaa cagaattttt
    30961 taccagcttc aaccgccgct ttcttaccca aattgcccgc atactataac acaggataag
    31021 atttcaggaa agagagcaaa aatgactttt tttctattca ggataacaac tcggattgac
    31081 gatattttat gtctgcatat gtcccgataa aacagagttg attttggcaa aattcgattg
    31141 tatcattcca agctggagaa tcagattttt cttgcagaag gtaaagacta tgtcccgcgg
    31201 agaaccgctt attcggcaat ggaatttatt gaaagccctt caggcttatc actatggatt
    31261 gagtgcagac gaactggcct gccggctgga gtgcagcaaa cggcaggtcc agcgggattt
    31321 aaacgtcctt caagaagtcg gctttccgat ttcgtatgag cagcgggatt tcggcaaacg
    31381 attttggaag ctgtcgcacc attttataga gagcgataag ctgattttct cggttaccga
    31441 gatgctgagc atctttctga gccagaaact gctggcgccg ctggcaggca cacaatttgg
    31501 tgaaggtctt gactcgctgt ttgaaaagat taaagctact ctgacccctc gtgcattaag
    31561 ccattttacc caaatggaca gcacgctgct tgttaaaagc catacgttca gcgactattc
    31621 cgcccacgac aaaaccattc ggctgcttac caaagcaatt caggactctg ttgtcgtaaa
    31681 tctgacctat aaatcgcaga gccgtcagaa accctaccag actcgctatc atccgtatgg
    31741 aatggtcttt tttgacggcg atttatactg tatcggtctg atggagattt acggccagat
    31801 tcgaacccta aagcttaacc gcattatcag cagtgaactg accccagaca gatttcgcaa
    31861 acctgcagat ttttccatcc aagaacagat ggaaggctca ttgggcattt tcagctcgct
    31921 gggaccgaca caaaccatcc ggattcaact ggacggctgg gctgccagca gtgtccgtga
    31981 agccattcat catcccagcc agaagattct tgacgataat gacagccgtg ttattgtcca
    32041 gtttaaattg gccgatacca ccgaactgaa acgttggctg ctgagctttg gctgccatgc
    32101 ctgcatttta agcccaaaac agttagccga ccagatagca tcagaaatca agaaaatgtc
    32161 ccagcgctac acaaaaaaat aaaaaaaatt ttcacttttc gccatcacag catataggtt
    32221 ctttcttgtc gcaaaacccc aaagcgttgt aggtctggcc agagatgtca taccctgacg
    32281 caagttttca aaaaacagct tcgaccaaac ctgtcatacc cctgttttat actttggatt
    32341 gatgatgcaa ttgtctaatt ttgttaaaga gctgaaaaat gattaaattg ccttatccaa
    32401 ggaatgcacg gggattacgg cagctgtttt cggtcggttt gctgcgacgg atgcccctga
    32461 taactcgggc ggcatttgaa agaatctgga atcgtccggc agccggttat tctgcaaatg
    32521 cattgcatag gcggcagaag gagaaaagca tgaccagcat gacagacaac agagtgtgcc
    32581 gtcatcccat tgaggacgcc gccggcatta tgtcgctgaa caataaacaa gcgttctctc
    32641 attcatacag cagatgcgga acgcctgcag atcggcagca agggcaaagc ggcagaaggg
    32701 aaaagaaccc gttgcggcat tccgcacagc ggggggaccg caggattcgg tttccccgca
    32761 tcgtttttta catcggccgg caacctcaac tttagattaa gggaaaaatt agacgctccg
    32821 gcggccggag aaggcaacat cgctgcggcc aatctggaag gttaatatga tgctgctatt
    32881 tttaataatt ccaaagggct taaccgtgtc aacaccgatc agccgctgcc cggtcatcgg
    32941 tttaatgcaa attcaatctt gaaaagatcc cccagccgtc ggcggccaat catacaggtc
    33001 gcctccaaaa tataattctt tccagatgtc agcggagttt caacgggcag gagaaactca
    33061 taaccttcca gatactcttt ccaatgctgg tcgtttctat cggaatccgt taaatcctgc
    33121 agcggccaca atatgatctg tctgccgcga ggattggagg agacaggtga ataatcatac
    33181 aattcaaatc gcataacaaa cggggcacgg attgaaaaac cctgcggact aataagttcg
    33241 gccaatatct taagatttat tccatttccc gactccgccg gtacaaattc agtcaacgat
    33301 gaaatcctca ttttggccgg ctcatcggct ggtgcggcgc ttggggggct ggacagttca
    33361 catcccgtcc atcccacagg atcacgcccg ctgccatcgc caggacgatc cacagcggca
    33421 ggaagcaccc cctgtcattg gccttataaa tcttcatcca gcaaatgccc gaaacgaatc
    33481 tttttggttc gcagataatc ccgattatgc tcgctggggg aagccatcag cggtatttgt
    33541 tcagctacct tgattccgta cacttccaga cgggatattt ttttgggatt attggtcagt
    33601 atccgcacat gcgttaaacc aaggtcgcgg caaatctgtg caccgatgcc gtaatcgcgt
    33661 ttgtccggca gaaatccgag tttgatattg gcatccaccg tatccagccc ctgctcctgc
    33721 agcttgtacg catggagttt gttggtcaac cctatgcctc ggccctcttg acgcagataa
    33781 atcaaggcgc ctttgccctc ctgctgaatc attttcattg cggtgataag ctgatagccg
    33841 cactcgcacc gctgggaatg aaacaaatcc cctgtcatac attctgaatg cacgcgaatc
    33901 agaaccggtt cagggtgcag tatcggattg ccgccttcgt ctaaatcccc cactccgccc
    33961 ttgcacaagg ccagatgcgg ctcggttgtg ccgggactgg agtagccaat catcttaaac
    34021 tcgccgtagt cggtcggcag ccggatttcc tgaatgcgat gaatatgctg ctcccgctgc
    34081 agccggtact ccaccagctt ggcaatagac gtcattttca gcgaatgttt tcggcagtat
    34141 ttctccagat ccggtacacg agccattgtg ccgtcatcgt tgatgatttc gcaaatcacc
    34201 gccgccggtt ccagccccgc cagtttcatc aaatcaacag ctccttccgt ctggcctgcc
    34261 cgcaccaata ccccgccatt gcgtgcccgc agggggaaaa catgccccgg gcggacaagg
    34321 tccgacgggc gggaggccgg atcggctgct acccgtattg tgtgagcacg gtcagccgca
    34381 ctgatgccgg ttgaaatgcc ttcgcgggca tctacactaa cggtaaaggc cgttcccaat
    34441 cgggcagtat tttcgaccgt ctgggggtgc agattcaact gatcgcagcg ttcctcagac
    34501 agcggcagac aaatcagccc tcggccgtaa gtagccataa aattgaccat ttcaggggtt
    34561 gtcaactgag cagcacatac aaggtcccct tcgttttcac ggtcttcatc gtccaccaga
    34621 acaatcatcc ggccttgttt gagttctgac agaatttcag gaatttcact gaataggctc
    34681 atagggattt acctgttcga atctgatttt gctgtttcac caaaactttc aatatactga
    34741 attccgcgcc gaatgtaaat aaaagttgcc gttaacgccc ataagccggt tatccaccac
    34801 acaatccgtg tccatagggg ccaaatctcc agcccgcggg ccatttccgg ccccatcagg
    34861 atagacaaaa ccatgagtat ctggaaagct gtcgatgctt tgccaaccca taccggctga
    34921 atatagatgc tgcctgtcaa aaaataggtt attacaaatc caagaagcag cagaatatcc
    34981 tttccgataa tcaaaacaac caccgtcagc ggcagtcgga agccctcaac agccgtctgc
    35041 gggagactca aaattataca agcacaggta atcagcagct tatcggccag cgggtcaaga
    35101 aatgttccga gcggcgttgt ctgtttccga atgcgtgcca gaaagccgtc caaagcatca
    35161 ctgccggcag cgatcccaaa aagccccagg gccgcccacc ggaaatataa tcccgctgaa
    35221 ggatcgcccg ttttgatcag acaaatgaca aatggggcaa ccagcagtac tctcaaaatc
    35281 gtaatctgat tggctattgt aaaaagcatg gcagacagcc cttattttac tttataaatc
    35341 ctcttgagaa aaggacaaaa acagcatacc ggcaaatcca tcacattcaa ctatggattg
    35401 ggacgggacg gacttagcag aaaagccgat aaaggtgctg ttgcggcgga cttgctgcgg
    35461 tgccgttctg tgcgaatttc ttagaattgc cgcaggaaac ggatgtcgtt ttcaaaaaga
    35521 tgccgaatat cggtaatccc gtattttttc attgccagcc gctcaatgcc gaagccaaat
    35581 gcccagccgg tgtatttttc gctgtcgatg ccgacggcgt caaagacatt cggatcaacc
    35641 attccgcagc cgccgatttc catccaattc tgccggccgg ttttgtccac ccacagaata
    35701 tcgacctcgc agcttggctc ggtaaacggg aagaaactcg gccggaagcg ccactgggtt
    35761 tgtgcgccga aaaacgtctg aataaactga tcgatgctgc ttttcaaatc gatcatcgat
    35821 acgccttcat cgactaccag cgcctcgagc tgatgaaaca taaacatatg cgtcgcatca
    35881 accgtgtccg gccgatatac ccggccgggt gctaccacgc ggatgggcgg tttctgcttt
    35941 tccatcaccc gaatctgaat tgtcgaggtt tggcttcgca gcagcatgga ttcattgatg
    36001 tagaaattgt ctgccgggtc tcgggccgga tgctccggcg ggatgttcag agcaataaaa
    36061 ttgtgccact catcctccac ctccggccca taagccacat cgaagcccat ccgcccaaaa
    36121 atctccagaa gttcggcgat ggtctgggtg ataatgtgcc agcggccctg ccggacctca
    36181 atgccgggca gtgtaacatc caccatcggg gctgttttgc ggccggcggt ttcaaaccgc
    36241 tttcgggctt cttcaaaagc gtgggtgatg tcatttttga tgcgattggc caactggccg
    36301 ccggcttttt tgtgttccgg cggaagtttc ccgatcatcc ctagcatctc tgtaattaat
    36361 cctttgcgcc caagatactt gattcggtaa gcttccagcg cttcggctgt cccaacagca
    36421 tcaagctctt cttgtgcctg tcggccaatt tgttcaaact gctccagtgt catccgggca
    36481 tcctctcggt tttagaaccg gctgccgcct cgggcataag cggtttatgc cagagcggca
    36541 cgcacctttt cgataatctg gtcaaagccg gccggatcat ggatggcgat ttcgctgagc
    36601 atcttgcgat taacggcgat ctgggctttc ttgaggccgc tgatgaactg gctgtagcgc
    36661 atattccgtg cccggcaggc agcgctgaga cggataatcc acagcccgcg atactggcgt
    36721 tttttctgac gccgcccaat gcgggcattg gccatagcat ttgtccgggc ttccttggcc
    36781 agacggtact gtgtatgggt tgcaccgcgg tatcctttga ccgctttgag aatgcgtttt
    36841 cttgcttggc gggtggccgc ccctttactg actcttggca taccagcttt cctttctgtc
    36901 tgtaattgaa tcggctgctt cggcaagctg atggggccgg gcagcagcca gggcaaaaaa
    36961 cgcccgtgtt acttgttcag caaaatacga atctttttgg ccaaaacgcc tttgacgatt
    37021 tcctgtccgc cgcttattcg gcgttctttt gaacttttgc cgctgagcag gtggccatgg
    37081 ttgcagcgat ggcgttttac cttgccgctg ccggttattt tcacccgctt ggcaagccct
    37141 ttatgtgttt ttaatttcgg catataatat tttcctgata ttgaagttat aaaaaatcac
    37201 atccatctct tgaaagacca cataagataa ttccggcccg gcggcttgtc aaccaaaaat
    37261 ctcaggaata ttttacatct ccaaaaaccg gatggcatgc aatggactat ctgaacggaa
    37321 tactatccta acatcttaat ataaaaagtt ttatgcgata aaagaaccgc acaacatcca
    37381 tcgctttaat agcgtgttct gcaaaatagt aatttcaaga catatttcga tttgaatcca
    37441 gcaggcagtc ccctctgcat acagaactat ttcctccggt ctgagtagca agatacttac
    37501 cctacatgga cagtgttaaa gtaatctttc ctttgaggac cgtatttaac caatttctcc
    37561 acccctataa aatagctgtt gataagtaat agaacaggtg tataatcctc cggcagggcc
    37621 aaagatttta gttttctttc ggcactttcg actgaccatt gagaacctta agtgtttccc
    37681 gattaacagc gcttctcttt gaatcgcgcc accatcccca tttgttaaaa tatttgacag
    37741 ccgaaacaag gtgattccaa agcagccgga gatccttata agaacctttg cgataatgat
    37801 gcattaccgt tgcataaggc caataaaccg ttctgtattt gcatccgata cgccggacca
    37861 ggtctacatc ttcaaaatac agaaaaaaac gttcatcaaa aagccccacg ttccgcagaa
    37921 catccgtccg cataaacaga aagcagccgg acagattagg acagtcaaac ggagccccat
    37981 aatcggcatc ttccagctga tagtaatgat tgtactcagc tgccagcaga ggcagaaaac
    38041 gccgtttgaa cagatgcccc ggtgtcggca gcagcttgca caatctctgg ggcgtaccat
    38101 caggaaaaac aaatctcggg gacagcagac caatcgtgct gtccgtattc atcgcttcga
    38161 caagcgtctc aatcgtcccc cgctcaaaag agacatcggg attcatcacc agacagaaac
    38221 ggcttgtacc ttcttccaat attatcttta acacccggtt atgtccgcct ccatagccgc
    38281 tgttttcact gggcatatat tccagatgcc cccaaggagg aaaccttcgg cccaatccgc
    38341 cgactacggc ctcaacagaa gcactgcgcg agttgtcaac cacccagcag gtaaaaggaa
    38401 aggtaatatg cgatacactt tcaagacaat cagaaagcac ctgcggttcg gtatgataag
    38461 cgacaatgga aatcccgatg tggtttgtat tcatttggtt aaagtgttga catttctacc
    38521 atttggcgaa acttttcata tttttccgac aggctttcaa atgtgcctgt atccaccagc
    38581 cgatgcttgt caaaatagag aatccggtcg cagcttttta atgtcgataa acgatgcgca
    38641 atcgctatga tagtctgccg gcctttaaga gaactgatag aacgggcaat ttcgctttct
    38701 gtcggcacat ccagagacga tgtggcttca tccagcacca gcacatcggc ctgataatac
    38761 agggcccggg caatcccaat acgctgtttc tcgccgccgg acagtttgcg gccattttcg
    38821 cccagcgtgg tttcaagctt attgccgagt cccatcacat aatcatataa attggccatt
    38881 ttaagaacct tttccactgc ctctacgtca atttgatcat tgggaaggcc gaatgccaca
    38941 ttccgaatca ccgtgtcatt ggcaatataa acctgctgcg gaacataccc caaccgccgc
    39001 tgccagcggc gaaccgtatc cggggccaga acagccgaat ctatccatac ccgcccttcc
    39061 tccggctcca aaagccccag caggatatcc acaaaggttg ttttccccgc tccggattcc
    39121 ccgacaatgc cgacatattc tcccttcttt atcaccaccg aaacattaca gagggctggc
    39181 cgcttggccc cggggtaggt ataagaaaca ttttcaaatc ggatttcttt ttgaaaatcc
    39241 agcggtgcag ggtcctgtcc ggccgaattc ccccctgcgg cctgaagata gtcccgccac
    39301 aaaggactct gcacttcttc cagaagcact tcgacggcat gctgcgaatg gttaatctgc
    39361 tgaagtgctt ttagaatccg attgacaatc gaggacgtac gaaatgcgat ggccgccaag
    39421 atgcccagag aagccaccat cgccggattg tccccgagag tcgaatgaat cacgccgata
    39481 cacattacca cgacacacag gatagcggta atctcggtaa ggtgatgggg gaactgatga
    39541 taaaactgcg tccacatatc attatccatt gtggcgcggt tgatgccgtc aaagctgtcc
    39601 agaaaaaact gttccctccc aagaactttg gtctccttga tagcgtgaag tccctgaaag
    39661 atatttttat actgctcgga cattaaggca ctcttttccg ctcccagcga tttgaatttc
    39721 cgcttgagaa aataatactg gcacaaggat gcagccgtta agatgccggc ggtgataact
    39781 gcaaaaagaa aaaaacgggt tatcagcacc gacagaataa tcaggccggc aatgatattg
    39841 gaaataagca gaaatccctg caggacaaag ttgtttaagg catgaactgc aatgtgattc
    39901 acattgcgtg taattttttc cgtagttttc agcagatgca gcttgtaggg tgccagcaga
    39961 taaaaacgca tcagagcagt gctgatttca atcttccagc ggcgaaggat tttgttctgc
    40021 caaaaataat aaaacagaag atagatattt ttagccacaa acaaaaaaac gataagcccc
    40081 cccagaaagc aggcctgctg cagcggggac tggacgccaa acagccgcat cagctccatc
    40141 agccaccggt gatgacgcac cgactcggga cgtaccagaa tccgtataaa cggcagcagc
    40201 agcatcaatc ccgcaatttc cagcagcccc gcggcagcag ccccgacaat aaccagagga
    40261 atataccgct tatatccctt gccgaaatat ttaaagaatt caatatagta tttaatcata
    40321 agccgtcttg tatcctgttg cagccgctat ttaacagacg acttcatctt gtgttccatc
    40381 cgcctcacaa tccgcagtgc cggccgctgc agaaaggaag gcatacggcg gtaaatccgg
    40441 acagcaatca taccggctct tctgacctgc tgcatatcca tttcaccatc ctctatcgcc
    40501 attgccttga cgtctttgga gaagcaaacc agatatctgc ccaaaacgga gcggatgcgt
    40561 tgggattcga tgcccggcag cagccgcagt gctgcctgcc gggcattttc ttctctggcg
    40621 gagaatgctt tcagcagacc ttgaattata gtctccagcg cagcaggata atgttcctcg
    40681 agataggcat tgatttgctt ggcatcttct tccagcaatt cctcataggg ctgccagtct
    40741 tcattgggat ccggtagagg atcgtaaaac agctggccgt ccggccgccc gagatagtcg
    40801 cgggcaatcg cagcactttc ctccgcatac cgcgcaagaa gctccaaccg ctgggccggc
    40861 gaaaataccg aataatcctt ccgcccctgc tgtatcatat agtccgatac cgcgcgaaga
    40921 ggattcaccg tgcttcgctt ttcagaccgg gaaaggggga gcaaattaac cagacgttta
    40981 tattcaacaa caacccgatg aagacgcgta ttgacttctt tctcaggaag aacaaattca
    41041 cccgtcagtt ccagtcccag aatatgatgc aggaaatccg ccgccacatc gccgcgatag
    41101 agctggcccc tttcataggg tctgacacag agatgttctt ttccgaagac atcggcccac
    41161 ctgtcaagac agcatttggc cggcagattt gtcaggaatt gcggccggtt ggttatttct
    41221 ccccctttaa ttctttgatt ataccacgag tcttccagcc cgtcacgtct gcgccaatag
    41281 acgacaatct gaacatcata atccctcaga agcgatttta attgggaaat ctgccgacca
    41341 gaaaatccta aaaaatgttc tgaactcaat atacccgaat tggatgaaat ctgccgccgc
    41401 agctgctccc attcccgcct tggatgctgt aaatcgtctg gccaatctgg cttttttact
    41461 ttctgcgccg tcataataga ccagacgagg cgatggtggg cagccaaatt ttcgggggat
    41521 tttggataaa aaatccccat cttctggagt aattccctgt taaggcctaa aaaacgctgc
    41581 agtgcagaag tgccggtttt aaccattcca atatgaacat acagccgact ccggccggcc
    41641 atgtcggcac ctgtcttctt acggactgtc aaacggctgg cagcagcagc cgttgcaacc
    41701 gcacatttta taacattaga aggcaatcgg ttgtaaagcg gccgaaaata ctggagaacc
    41761 tcccacatgc gggatgcgta gaggtcatca aaagcagctg tttgctgact tgctgtttga
    41821 atcggcggca tcaacacggc cgatacagca tctgctttga aatattctga cgcaatcccc
    41881 ggcgacagcc ggcgggcagc caaccgcatt tgcgggtctg cagccgtttg agcccggaca
    41941 atgccggcgg caatcacacc cagcacagtc ggagcagcat cggctaaaca gcgattgatt
    42001 tcggcagcat cttcttccag cagatctctg taaggccgcc acagatctga ttcagacggt
    42061 tccggttcat aaaaaaacat tccgtcatcc cgtccacagt attcccgggc aagataatgg
    42121 tagaatttgc ggtttttttc aatctccctg cggcgcagcg ccggggaaaa cacgaaatcg
    42181 ctggatttgc ctttctgaaa aaacaattcc gatatccgct gcaaaggttc aacagctgca
    42241 tatatttgct gaaggcttaa gggcagatga ttgaccagcc gtttgtattc gagaacaatc
    42301 cggtgcaaac gcaggtttaa gtcggcaggg attttgtatt cttctgttaa ttcagcattc
    42361 cagacagagt tgagaaaatc ggcataaata ctacccttat aaaactggcc tcgctcatac
    42421 ggacgaacaa ttatcgaatc gcggccgaat gttcgttccc acaaggcaaa tttctcccac
    42481 ccagcgccga ttttcacatc tgttcgcttt tcaaccccca tcttgacctt ttcggcatag
    42541 agagattcct taaagaaatc ctgccgtcgc aaatatacaa caacagtcgt atgataggaa
    42601 cggctcaaat ctttcagaaa agcgacatcc tcccgtgtgc agccccagaa ggtttccgta
    42661 ctaatcagat ttcgaggcag cacacactgc cggctgatct gctgccattc gaaatcacac
    42721 gtgcccacat ccctgtcgaa ccaccaatgc ctaaaacctc ttttcaacct taatgcccac
    42781 ccgatgcgat ggtgggcttc cagacccccg gcagcagggt aggcaatccc tttctgtgcg
    42841 agtagatccc ggttgatgga aaaaaaccgc tgcagcgccg aggtagccgt tttgggcatt
    42901 ccaatatgta agtacagttc ctttgaattc atatatccct gcttttgtct gctactatat
    42961 cccaaaagtc ttgccatttc aagaacacaa tcggcagtac ccggctgttt gagattctgg
    43021 aaagacttgc ccttgacaac ggaacacttt tgctctataa gttccgaata tgcagaataa
    43081 taagcgtaat ggccgcagac aggtcaggaa tggtccatga attatgatta tcttattgtc
    43141 ggcgctgggt tgtccggtgc tgtattcgcc cagacgctgg ctcaggcagg ccacaaagta
    43201 ctgattctcg aaaaacgtag tcacattggc ggcaactgct ttgactgtgt agacacggca
    43261 ggtgtgatga ttcatcggta cggggcacat attttccata ccgagtttga ggatgtctgg
    43321 cagtatctgt ccggctttac ccgctggcgt ccgtacagcc atcgggttgt ctgcttcgtg
    43381 gaaggccgct atatccccat tcccttcaat tattactcac tgcaagaggc ctttgacggc
    43441 aaggcagcag ccatccagtg tcttttagag cagaaatatg gacggcaaac aaaattgtcc
    43501 gtgctcgata tccttcattc gcaagaaccc atgcttggca atttagctgt ttatatttac
    43561 gacaaaatct tcaaaaatta taccgccaag caatggggta ttcgtgtcga cgaaatatcg
    43621 ccggatgtca tcgcccgggt acccatttat acagactacg acaatcggta ttttacggat
    43681 acttatcagg ccgttcctgc agacggctat acggctatga ttcaggcact gctgtccgac
    43741 ccgcatatcg agctgaggtt aaataccccc ttcgaatcgg tatgccagtt tacggacggc
    43801 atcattcgtc tggacgggca gcgattcaat ggaatactga tttataccgg ccggcccgat
    43861 gctcttttcg gttatcgttt tggtctcctg ccctaccgct cggtacattt tgaattcgaa
    43921 acatttgccc aaagctatta tcagcatgct cccgttatta actacccaaa cgattacaac
    43981 tatacgcgta ttgttgagtt taagtatctc acggggcaga aatgtccaaa tacaactatt
    44041 tgccgggaat atccgcgtcc ctgtcagacg caggccgatg agccatacta ccctattctc
    44101 acccccgcat ccgttcggct gtatcagctc tacgccgagc aagccagaca gtacgacaat
    44161 ttgattttgg ccggccggct gggacgattt cagtacgttg atatggatga tgcggtcaaa
    44221 aatgccctcg atgcggcaag gacgcttttg caatgccgcg tttaacaatc ctcatccctg
    44281 tttataatca ggccggctat ctgcctttat gccttcagtc tatcggcgaa caggaattca
    44341 ccgattttga aattattgcc gttgatgacg gcagctctga cgggagccgc tctattctcg
    44401 atgcctatgc cgcagacgaa agacgaatca gagtcatcgc gaaatccaac ggcggatacg
    44461 gcagcgccct gaatgccgga cttgcggccg caaaaggcga ttatatcgga attgtagagc
    44521 cggatgatac catcgaagcc ccaatgtacc gggtcttaat ggccgccgcc gaacaaacgc
    44581 aggcggatat tgtaaaatgt tcctttcgca ggattttctc cgaaggtctc tgcctcgaag
    44641 aaaagggctg gtacgccgcg gccggcagag ttttttgtct ccgccagcac ccggaattgt
    44701 gccgcctgca tccggcgata tgggctgccg tttacaggcg gcagcttctg gaagacaatc
    44761 atatccgttt tgctgaaacc cccggggcta cctatcagga tgtgccgttc tttgcggaag
    44821 cgttttccgc cgcccggact attttcatcg tcccgcaggc actctacaat taccgtctcg
    44881 aatccgaaaa cgcgtcttcc tcttccaatg ccatcgatgc caaagtcttt taccggctgg
    44941 aaaatcatca gcgggcgcgt cgtgtctatc aggcccgcgg actgtgggaa attgtcaaat
    45001 attatgagct gcagcgggaa tttatcacgc taaataattt cgcgctgcgt atcaaaggtt
    45061 cgctgcggcg ggcactgttt gaaaaaatac gcggctattt tgccgagatt cctgtcagcg
    45121 aggcggtgtc gtttctgccc tcgggattcc gaacctcttt tgagctgatc aaaaaaggca
    45181 gctacagccg atggatgctg cagtatgctc tgctgcctgt tataatcatc cgccttgcag
    45241 aacagcttgg gctgtcagac cggctcaggc agaaatacta cacatggaaa gataccgggc
    45301 cagccgccta tgttcaagat ccagaatata gttactgaca atcagatgct tcaaaactgc
    45361 agccgcttgt tttacacgca ggagcatcgt ctcggccgca gcatgctgag ttttcagact
    45421 tatttcaaca cactctggct tcaagcatgg aaggagtaca cctgcgccac ccgcttttat
    45481 ctgaatatac gatttcgcgg ccaatgcagc atccggctga ttcagagtca tctggcagaa
    45541 gtaaaatacg attcggcttc cagcagcccc cttggcgaaa tagcgtccga acaccttttc
    45601 ttcgaacaga ccttttgttc ggacggatcg gactatcagc agattgaaat caccggccat
    45661 gatgccggcg cccgcatggt ctatttcaaa atagaccatg cgagcgattt tgaatacatc
    45721 gatgcttttt actcggtcga ttctgctttt ccgcctgctc gtgttaaaac agcgattgtt
    45781 atctgcacat tccgccggga gcaatttgtg cgtcggaacc ttgagatttt gacagcatat
    45841 ttccgcaagg agccggactt gcgcgaggtg tatgatgtct ttgttgtgga caacggccgg
    45901 acgctttcgg cagaccttgc acaatatcct gtttttctct ggcccaacaa aaacacaggc
    45961 ggcacaggcg gttttacccg cggcatcatt gaagcactcc gctccgaccg aaattatacc
    46021 catgtgcttc tgatggatga tgacattgaa atttttcccg aagcgctgca gcggacgtat
    46081 gctttggccg ccctgctcaa gccggattgg caccggtctt tcattgccgg cagcatgctg
    46141 cgtgcggaca tgcggcattt ccaatgggaa gcccgctcgc acatcaaagg actccagctg
    46201 gaggcttacg gccggctttt tttgtcggac ttttcgtcgg tcgtaaaaaa cgaaatcctt
    46261 gccagccggc ataccccgcg ggattatgcc gcctggtggt actgctgcat tccgctggca
    46321 gcgatacagc cggacagatt gcctcttccc ttctttgtcc gcggggatga tattgactat
    46381 tcactcgcat gcgccgataa aatcattcat cttaacggca tctgcgtctg gcacgagcca
    46441 tttgagagcc gccgaaacaa tgtcgtggat atgtatatga ccgtgcgcaa ctttacagcc
    46501 ctcagcatcc ttcataaaag ccccctgccg cttaccgtgt atcatctgat ggctcttttt
    46561 gccggaaata tcttcaccta caattataag ggcgcgtttc tggtatgctg cggccttcgc
    46621 gacgctgtgc gcagaatttg cagttttgaa gacaatcaga cggagctgct tgaccgttat
    46681 gcagcttaca atgaaatcga aacaaactgc gccggcgacc cgtccgaggc ctttaaaaag
    46741 ataaaactcc atgtgccggt caaattgctg attgtcctga cctacggcgg ccatctgctg
    46801 ccgggattct tttttagaaa aaaaacattt gctttggccg gctatcgggt tgcaattgca
    46861 aaatgttttg ccagcagaca cgtctgtatt tataatccct tcaccggcaa atggatagaa
    46921 cgaaaaatcg acaggctcgc ggcttttgcc cttggctttc ggtttctttt tctgcttctt
    46981 tatgttgtct tgttttaccc cagcatacgc aggaagctcc ttcaaaaaga agcccaatac
    47041 cgaacactgg aattctggaa acggtatctg gagctttaac ctgcagcagg acaagtctaa
    47101 cacagacgga ttcacctccg gacagttctg ctgaacgcat tcgctgctct ttttcgcagc
    47161 agattcttta ctttttgacg ggggattgta aacttgtgaa ataacagccc agcccgataa
    47221 acgccggcgg actgtaaaag cccaacatat aaaacagata tgacaatgaa cagcagacag
    47281 ctttcagatt atataaaagt cagacaagat tcatggaggg gatatatcca gagagatttt
    47341 gcggacttgg agccgggggt tctggccggc gaaactccgc tggacagccg ccgcagcgct
    47401 ctggaaagag tagcctcatc ccataccgca gaggtatttc gctatgctgt tcaggcaggc
    47461 ggacgttcct atacgctgta tctcaaaaaa tttccgcccc gttcaccagc ggactggttc
    47521 aagcacctgt tccgccccag ccgggcacag cgggcattcc gggcaggatt gctgctgcgg
    47581 cagtacggat tcggcactcc gccgattgcc gcctgtcttt gccgaaagaa aggcctgcta
    47641 atgagtcagt atatccttat cacgcaggaa atgaagcagg cccatccgct ttatctcgct
    47701 ttggaatcac tttgcggcct gcaagacaaa cgccggctga ttgccgaact gggcagaacc
    47761 atcggccgga tgcatgcggc aggcatcacg cacggtgatt tgcggggcgg caatgtattc
    47821 gtttctcaac aggccggccc ggccgtcttt ttcttcatcg acaatgagcg tacggttcgg
    47881 tatccgattc ttccgggctg gctgcggatt aaaaatcttg tgcagcttaa tatgctcgaa
    47941 aaaaatatta cctcttccga ccggatgcgg tttttaaaag cctatgccgc cgcagcgggg
    48001 cttaagaaaa ggacaatgcg gcggcttgct cgggcagtta tccgaaaaac gcagcggcgt
    48061 ctcaggcatc gggctaaaac ccggctgggc tatgcagaag gaccagctga ccactggaat
    48121 tttcagcgga cccgatggga aaatcgccgg ggaacatttc ggatggattt ctgcaaaggg
    48181 acatctgccg tcgcattcct gcgtgagatc gataatctaa cggaacacgg cgttctatta
    48241 aaaaacgata ccgccgcccg agtggttcgc tgcacgtata acaaccggga tattgtcatc
    48301 aagcggtaca atcatcaggg cctgtggcac tcgctgcgct ataccttcaa aggcagccgg
    48361 gcaaaaaaat gctggcggtt cggccatcgg ctgacggagg ccggcattgc ctgtgccgct
    48421 ccgctggcgt gtatcgaaca atatcaatgc ggactgatat ggacatcgta tatcgtcaac
    48481 gcatttgtcg aaggtccgct gctgtacgat gtgatgaacc ggccgggcta ctcgccgcag
    48541 gaaaaggatg cagtgatgca acaggcccgg cagctgctgg aagaccttgg acggcaccgg
    48601 ctgacccacg ccgatatgaa accggcgaat ctgattattc atcatggaaa gccggtgctg
    48661 attgatctgg atgcgatgca ggagcaccgg atgcggctgt ttttccagta tcggtataaa
    48721 aaaatggtcc attattttca ctgccgcctg cacggcaaaa agaggcaaaa ctaagtcgct
    48781 gttttgacaa tcagttcgca cagctttcgg ccaacagcgg aaatatcata atttcgggcc
    48841 tgctggatgc cgttttgtcc catttgacac cgcagggctg gattttcaat cagcagcgag
    48901 agtttttcaa gccactcctg ttcagttcgg accaaatatc cggtttggcc gtcgatgaca
    48961 tgatcgggat tggtgccgat aggcgatgca accaccggca ggcccgaagc ggaatattcg
    49021 agcaccttaa aactgcattt gccgcgggta aacggattgt ccggcaaggg tgccagtccg
    49081 atatcgcact gtcccagaca atacccgcgt gcatgcggcg tccatttcat tttttcgatg
    49141 gttacgcctt ccatgtcaat aaaatcgtcg ccgatcagac gcagcacaag gccggggtaa
    49201 cgagccgcca gtttctgcag caccggtttc aattccgcaa tatatccgag ggtcgccctg
    49261 ctgccgatcc agacaaggcg aatgttgccg tcatcgcttt tctgcgctgc aggatggtaa
    49321 tcgtccgttt ttaatcccag cggcaggata atgatattgg agtggtacgg gcgggcctgg
    49381 tcggcgagat aggaactgcc gaccaacact gcatcggctt tctgaagaga acgccggaaa
    49441 cgcttttgat gagccggtgt cggacagcct ttgtcattaa acatcacggc gtcgtcgtag
    49501 ttgaatatca cctttgcttt tcggggtcta aaaaaccgcg catccaaaaa attcaggcat
    49561 tttttatgca gcaggacgac atcataggtg tcggcctgtc gatattggcc ccagcgtttc
    49621 agcgggttgg aggacagcac cgccaccgtc ggctcaatcc cagcagcccg gaatatatcc
    49681 agatacctcc cgatgcggtc tttgaagctg ccccgctgag gattgtttgt caatatcagc
    49741 aatttcataa cgccttctct gagtcttgca atcggcaatt cagattccca aacttagaaa
    49801 cagcagaatc ggcagacagg ttaccagcaa aaacaaactt cgcaacaaaa aaaccgattt
    49861 caggttgaag ccgctttgcg atttcatcag tttaattttt ctgctgatta actgccgtat
    49921 gccgcccgat gcacaggaac ttaatatttt tgttccgctg ttttcatctt cgccggcttc
    49981 caccataatt atgcccgcaa gggcggtcct gacggcaagt tccgtttgtc cgtttgttcc
    50041 ctccgatagg cattctttcg ccagccgaat gaatattttg ccgatttcac caatcctgtt
    50101 tatcgggtca ccagccgctg taacagcagc aatttcttta cccaagttat cccatgttat
    50161 cgttttggtg caaaaggact gtacagatgg gctgctcagg ccgttcagtg attcttttaa
    50221 tcgttgacac tttgttttga taatatggtt tttgtctcgg ggggttaatt tatccagtac
    50281 ccgaaacgct tttttatgct gctccggtga ctctgtcagc atgagcgtcc ccaaggcaag
    50341 ccgtataatc gcatgtgtgt tatcgttttg cttcagcata cggcgtgcca tgctgccgat
    50401 gcggccgccg actgcctctg cctttaagtc catcggggcc atccgggcag ccacttcaat
    50461 ttggggaccg aaccatttcc agatatggcg gctggtattt gtttcatggc tgcgcccagt
    50521 ccacaggggt tcttccataa atcccacctt atatttctca aacaagcgaa tatgcaccgg
    50581 ccagtcatct agaaggaaat ccctccagat tggtataata tccttttcga aaacagaacg
    50641 ctttgccaaa agtccctgca gtgaaatgcc atggagattc tgaagcttga ggaagtgcgc
    50701 cgccttcacc ggccgatccg acaggcccct tacagtttat tgtgcaaaag gaatgggagt
    50761 ttttgccctt ccatacttat ccatattcgg atcaagttca aatgtcggac atggctcttt
    50821 ttccatcaaa agattgtaaa gtatcttaga gcgtccattc ggcaatatta ttgtatattc
    50881 tccgcttttt tcaggaaatg ggtaagagtc tttaaaatca atgatatatc gtgttccgtc
    50941 aagaaatgat aggtacagat agcgtcccaa ccgttcttct tgcacgggtt ttcttatttc
    51001 gggattttcc agacaactta atagcacctt gaaatccgct tcatcgaatc gcttaaacac
    51061 acgcgatttt tcaaggcaat accctacgcc gtcctgatac ggaggcggca acagccacag
    51121 catcgaggtc attgtctttc cttcaaaatc tttaatggat ttgccatccc aaaagggaat
    51181 ttcgttacat ttgttgctcc tacatccggc aaatagagac gtaactgata aaataaccag
    51241 cattgcacat ttcataaagt accgcctttc tttcagaaga attgaatcca gtcaagtttc
    51301 gtattcaggg gccaccattg aagcacatta tccgcccagc cgtcccgatt tagatcatct
    51361 ccccataccg ccgcccgttg ctcgttgcta tgagggccgt tgataagcgc atgctccaat
    51421 gaatgattgt tgccgtgatt tcgtataatg ccggccaggg cagcggggta atgctcggcc
    51481 tccggcagaa accagcccgt ccacacgggc gttcgatttc ccatataaac atgcacataa
    51541 ttgccctgtg gatccttata atgcgcgccg accgcatttg ccatgtcatt aaaattatac
    51601 gtattaacgc cgttcttcca ttccagaccg ctttcacagc cgatagccca aaattcctta
    51661 attttatttg tttgccacaa tcccaaagac cgcatgctgt gcccgcggat ttcccagtct
    51721 ttcggtaaag tgtcaggcac agatgggtaa atgataccgt ctgagcctaa gcaggaaaaa
    51781 attctgtctt catccgtgtc tatccaaaat cttcttattt tccagcattt gaaattggta
    51841 cgatgaacac cttcttttaa tactttgcga gtaatacgat cgcgttcttc acctatttgg
    51901 ctattcatat gcccaaccca atagacataa ttgagattgc cgccctgcag agcgatgctg
    51961 atattgtccc atgtcgcatt tttataaagc agttcttgat atggaatact cttgtcgata
    52021 agcgcatccc gaatggcctc caacgttacc ttaaaatcct tattcgcatc tctatccggg
    52081 cctacaatca gcgcacgaaa ggagttcggg tctatcttgg acatatccac gattgccata
    52141 agagacatat tctgtgtctc tgagttttcg ccgtcttcat aggataaggt cgcttccgcg
    52201 ccggcggggg tgctgatgaa cgccgaatgg cggatgtagc cggtgtcgga cagcctttgt
    52261 cattaaacat cacggcgtcg tcgtagttga atatcacctt tgcttttcgg ggtctaaaaa
    52321 accgcgcatc caaaaaattc aggcattttt tatgcagcag gacgacatca taggtgtcgg
    52381 cctgtcgata ttggccccag cgtttcagcg ggttggagga cagcaccgcc accgtcggct
    52441 caatcccagc agcccggaat atatccagat acctcccgat gcggtctttg aagctgcccc
    52501 gctgaggatt gtttgtcaat atcagcaatt tcataacgcc ttctctgagt cttgcaatcg
    52561 gcaattcaga ttcccaaact tagaaacagc agaatcggca gacaggttac cagcaaaaac
    52621 aaacttcgca acaaaaaaac cgatttcagg ttgaagccgc tttgcgattt catcagttta
    52681 atttttctgc tgattaactg ccgtatgccg cccgatgcac aggaacttaa tatttttgtt
    52741 ccgctgtttt catcttcgcc ggcttccacc ataattatgc ccgcaagggc ggtcctgacg
    52801 gcaagttccg tttgtccgtt tgttccctcc gataggcatt ctttcgccag ccgaatgaat
    52861 attttgccga tttcaccaat cctgtttatc gggtcaccag ccgctgtaac agcagcaatt
    52921 tctttaccca agttatccca tgttatcgtt ttggtgcaaa aggactgtac agatgggctg
    52981 ctcaggccgt tcagtgattc ttttaatcgt tgacactttg ttttgataat atggtttttg
    53041 tctcgggggg ttaatttatc cagtacccga aacgcttttt tatgctgctc cggtgactct
    53101 gtcagcatga gcgtccccaa ggcaagccgt ataatcgcat gtgtgttatc gttttgcttc
    53161 agcatacggc gtgccatgct gccgatgcgg ccgccgactg cctctgcctt taagtccatc
    53221 ggggccatcc gggcagccac ttcaatttgg ggaccgaacc atttccagat atggcggctg
    53281 gtatttgttt catggctgcg cccagtccac aggggttctt ccataaatcc caccttatat
    53341 ttctcaaaca agcgaatatg caccggccag tcatctagaa ggaaatccct ccagattggt
    53401 ataatatcct tttcgaaaac agaacgcttt gccaaaagtc cctgcagtga aatgccatag
    53461 agattctgaa gcttgaggaa gtgcgccgcc ttccccgatc gtataaactg gctggttttt
    53521 gtcgtatcac gctgataaag gcggccggta gtctgctgga gaatatcccc gactgcataa
    53581 acggcatcca gattgttttc ctccagatag cgtacctgtt tttcaatctt ctcgggctta
    53641 tagaggtcgt cttgggataa accggttatg aattcgccgc atgtcacttc aaatgcatcg
    53701 gcaagagtag gcccgatgcc tttattttca gaatgtacga gaagattaat ccccatttca
    53761 acagcgaact ttctgatata atcagattcg tcatggttag gcgaagcgtc atccaccaca
    53821 ataatttcga tgttcggata tgtctgattg agtatcgaaa agatcgcttc ccggatataa
    53881 cgacggccgt tgtagtaggg cactaaaacg gaaaccagcg gtgtattatt tccagtcatt
    53941 gatggctctg gctattatct gaatatcgtt ttggttcatc gcaggtgaaa tcggcaagct
    54001 caacacttct ttcgccagac gctctgctat cggcagagat aaatggttaa attggggata
    54061 acattcctgc ttatggggcg gaacagggta atgaatgtgt gtctgaatac cgttgctttc
    54121 aagatacttc tgtaaatcat cgcgccattt ggttgtaatc acaaataaat gccacacgtg
    54181 gctgctggat tcttgaggcg gcttaggcag aatgaccctc tcatttttta tgtttttgag
    54241 atagtactct gccgtcatcc ggcgctgccg gttgtcttca tccagccctc tcagcttaat
    54301 ccgcaaaatg gcggcttgta tttcgtccat ccggctgttg attcccggat acttattata
    54361 atatcgccga tgagacccat aatttcgcag ggcacgaatt ttctcagcca actgaccgtc
    54421 attggtcgtt accattcccg catctgccaa agccccgagg tttttggtgg gataaaaact
    54481 gtacccgacc gcacgctgtg gataatcggt tgtgatactg tcttcggaat atacagcccc
    54541 atgcgcctga gcagcatctt caataacctt caggttatgc cggccggcta gggtgcagat
    54601 tgccgccatg tcgcaaagct gtccatagag gtgaaccggc atcaccgctt tggtgcgttt
    54661 tgttatcttt tcttcaatcc gtcctgaatc gattaaataa gtttgcgtat cgggctcgac
    54721 aaaaactggt gttgcaccgc atgcggtcac ggcaagcact gtcgcaatat aagtattcga
    54781 cggcacaata atttcatcgc ccgggccaaa tccgtacgca cgaagaatca gggataaagc
    54841 atccagcccg ctggcaacac caacggcata tttcatccgg caatacgccg cgaatgctgt
    54901 ttcaaaagcc gacagctcgt ttcccagaag ataccagccg ctgtccagaa cacgcctgaa
    54961 aacgctttct atatctgcag cataccgctg attgacagcc tttaaatcaa gaaaaggaat
    55021 cattacatca ccctcgtttg ccacacagca atctcttagg gttttcttac gtttttggtt
    55081 tcattgactt t
//
LOCUS       DAOTBO010000008        54109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_495_length_54109_cov_5.225904, whole genome shotgun sequence.
ACCESSION   DAOTBO010000008 DAOTBO010000000
VERSION     DAOTBO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 54109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54109
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_54109_cov_5.225904"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3204
                     /locus_tag="PLE88_01820"
     CDS             <1..3204
                     /locus_tag="PLE88_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75235.1"
                     /translation="GNVRYHNGTPGRQFTEQILAYNAVTIDRSDMASPSASTYGNGDL
                     TLYEPGQDGLAVTEIDGQRAYHNKASRYQRIMLLNTMDLTRPYAVDIFRVTGGTTHDY
                     VWHGSIRYDQTAQCSFALTPMPGETPMLEGSETWYEPTSSGSSFPYYGFFRDMSSNPA
                     PGNFQISYLDTNRSSPRDTKLWMTSEPEQYTVYMGGRTPVPARVDSEPINWWVNGLWR
                     PSTIIRRRTASGPLHDLFVSVIEPLNNGVSRIASVERIPMAGSQLESAGLRVMFTDGR
                     ADTYIVNLHNPQVAGANTGSATVTTADGSYSLTGRIGMHTTGSGNARVWVMNASSFQY
                     PGGSFMPSNLYYSGQITGQTRILKGDSYDALITDTSLPLGTVLRGKYLSMTFGTLSGS
                     GTTGISQMFEIDQVMQSDGQYHIQLPSDHYLDITDAVSISGSGADIWAAADQFHYAWQ
                     SQTGDLTLTARVLSQTNTNAWAKSGVMIRESSAANSRYVGLYITPSNGVSMQYRDAAG
                     ANAIDFARTAGLTAPRWLRLVRSGNTFIGYQSVDGINWIQVGSSGVSISMPAAVLTGL
                     VVCSHNNSVLNTTTFDNISAELPLTSADIGAVGLAGSTAYSDITIEQVAPLRTFNGVS
                     TFEIALSASRAAAPPDTPTDLRAIAGNGQVTLSWTAAAASYIVRRSEISGGPYVIVAS
                     PMTAYYTDTDVVNGTTYYYVVAAVNEAGQSSDSLQVSATPSASAPTGLVARFAFDNGT
                     AADSSGYGNHGILYGGLTSIHDAQRGNVLSFDGVNGSYIRVPSSPSLNAVAKQITIAM
                     WLYTNTTAVRQLIEKGGTSGAAWYTAPWGIRLETNRQLLLKWGNADFLKSTAIPSNTW
                     THVAMTYDSTLGTNNTKFYINGLPDTQAHWTGAAAPNSYDLFIGTDFYNNTSRWAYLG
                     CMDDVCIYNRALTPGEIAELTTAPAAPTGLAVTGGNSQVDLAWNASPGAVSYAVKRST
                     VPGGPYTTIIEGIMTLHYSDSTVVNGTRYYYVISASNSAGNESTNSAEMSAVPHRPGD
                     LTLDNAVNMDDMAALSAGWQTLYEIETMFEIAANWLD"
     gene            3223..7482
                     /locus_tag="PLE88_01825"
     CDS             3223..7482
                     /locus_tag="PLE88_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM75236.1"
                     /translation="MGKGISMVFVTTVFYAVLLWAVPAISQTDADILSIPGIELTERQ
                     KQPDLAWWRQSMQNRDQRLTWWRKARFGMFIHWGVYSGPGGEWKGQKVSGYAEHLMRK
                     CKIPRQEYLEQVARKFNPVDYDPAEWVALAKAAGMGYIVITAKHHDGFAMYDSAVSDF
                     DIVDATPYGKDILARLRDECRKQGIQFGVYYSHAFDWEHPNAPGNDWEYQNPGGDLGL
                     WGGVRWYDQHPEMVERIRKYVDEKSIPQIRELINNYHPSIIWFDTSSKLPGSELLRIL
                     KAVRQESNDVIVNSRVGGEGGNYGDYRSTADRPAEFVPVRESDWEGIPTTNESYGYSK
                     HDKSHKPAAHFIQLLAKAVCRGGNLMMNIGPMGSGKFASEDIEILNGIAEWMKYYRHT
                     VIETDRSPIPLQNWGAVTRKGNTLYLHIFDWPEDQILLLGGLKNRVRSVNLLDRSEKK
                     TLSFERLNPFDIQISLPPAPPDKVDSVVEVVFEGTPDGAASRLLDSRVPNRLHVFDSD
                     LHDRVRFMDGKTNRDCTQEWNDPRAVISWKARLIQETVFELQLISVENTAGQGRYELK
                     VDNQIFSEPVSKDSAVTTAGRITLAAGEHAISIRPLDVGQGELTRLRRMDLIPIKNPP
                     LKMWYSKPAQKWEEALPIGNGRLGAMVFGGSKKEHLQLNEDTLYSGYPGYRDVKLNIY
                     ESLPKVVEMLNARRFAEADDFITKNWLGAGQACYQPLGDLYIDFEHSEQVSNYRRQLD
                     ISSALCRISYTADNILYQRDIFASYPDNVIVIRLTADKPSSLNFSIRMDSPHPTAAVR
                     ADDARHLEMTGQVPGLVLRRTLKWVEDRNETWKYPQIWDENGRRRDPEKTIFYGDAIG
                     GEGMFFHAAVSVSLRGGQTKAAADRLTVNGADEAVILFNAASSFNGFDKNPVRQGLDA
                     AAKSKGFLRAAGQYSYKDLLGRHTADYKALFDRVWLDLGGWNEYAEMPTDQRIAQFGQ
                     VRDPMLITLYFQFGRYLMISGSRPGTQPLNLQGIWNPHIVPPWASAYTLNINTEMNYW
                     PVDICNLSECFEPLAQMIQELAVDGRKVASEVYHADGWVSHHNTSIWRDAQPVDNVTR
                     TSFWPMSSGWLCLPLYDHYLFTGDEDYLRSRAYPLMKGACEFYLDWMVEDSQGFLITP
                     VSTSPENDFFYTDAAGKQQRAGVCRGTAMDMAIIRELFANTIEASRRLQVDAEFRSLL
                     QNKLPKLRPYQIGSQGQLLEWQEEFAEPEPAHRHISHLFGLHPGTQITPLKTPELANA
                     ARKTLQLRGDGGTGWSKAWKINFWARLEDGDHAFKMLCEQITKSTLTNLFDTHPPFQI
                     DGNFGGTAGIAEMLLQSHAGQVVLLPALPSALSSGSVRGLRARGGFEIEMDWTDGCLT
                     SARIKSLLGNPLHLRYRSKSAAYHTQSGQMLTVNSNLEEVQ"
     gene            7515..9830
                     /locus_tag="PLE88_01830"
     CDS             7515..9830
                     /locus_tag="PLE88_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOM75237.1"
                     /translation="MLKKCVLLFVLTAVFSSACFVLAVPETAKLQIENMHTAVVFDNQ
                     TKNAAVYSKDAQKTYISSIQFKEALATAHRIDLNDTGFGKGQAIEIVTVSGDRQRLAL
                     YDRLPFVFMQSTIHNASAAIKTIPYYAPCVLKLDLQKSPRQLKAHGTAGLTAVDKKSN
                     SGSYAFLSIVDPDTRKGVVAAWLTNERGSGVLFSDIQGTSVILEPRIDYGRLQLQTGQ
                     SETLETLLVGWFDDVRLGLEAYAEAVAQKYAIVLPPQPTVYCTWYHAGASNEKDLIQN
                     AAFAKEHLAPFGFSVVQIDDKWQDGKSTNGPRKNFTQVQPNGPYKSGMKTAADNIKKL
                     GLTPGIWFMPFAGTFDDPYFADKQYLFAKKDGKPFDTKWGGTCFDLTNPKTQQYVYDI
                     VHRIAHEWGYQYFKMDGLYTGTAARLMYINDKYADDQLGESVLYDPSMTHIQSYRTGL
                     QIVRKAAGRDIFFLGCCIPQNMRSFAPAMGLVDAMRIGPDNKRTWEAMLRGPRYGSKV
                     YFLHRRVWYNDPDPIYVQDDVPLQQAITLCSWVTISGQLNASSTDYTKLPPERLDILK
                     RTMPAHNLTPRPADIFEQEIPRIWLLSDERSGVRRDVIALYNWDDKNTAEIHYPLRKI
                     GLDGEKQYIGFDYWANTFLEPFSGSVKVSLPQAGCSILSVRPVSSNPMLLSTSRHITQ
                     GIVDVKEEKWDESLKTLTGVSEVVANDSYELRIAAMKGNSIWQLKSVSVSEPDRQAGT
                     AIRIISQNGPFIRLLIETKESRQVRWNAEFN"
     gene            9855..11150
                     /locus_tag="PLE88_01835"
     CDS             9855..11150
                     /locus_tag="PLE88_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOM75238.1"
                     /translation="MNRMYQLRYRANYSAGIIFFIASAFLYAASALEDAAATAVPDAH
                     LDYIPGQAPAFWSIAAAETVMARWPDYSKAYFNAWTYVNGYMLCGFERLYRDTGERRY
                     FDYIQRYIDLFVDENGAFRSVLNSKGNLQKISFTNLDNMMTGNTLVMLYEYTRDERYK
                     KAADTIRRALDDYPRNSDGGFWHARGMHGQMWIDGIFMGQMFLTRYGKSVGDSRYCWD
                     EAARQITVYAKRAQRDDTGLYLHGIYEPGHGDRVCRWADPNTGLSPEVWSEGLGWYAL
                     IVVETLADMPKDHPQRPEIEAIFRRLAVGLKRTQDVPTGRWFQVVDKGDRPDNWTDNS
                     GSAMFTYALQRGIELGLIDKNEFEPVVRKGYHGIVENARINAKGLVDIYSACDGLGVQ
                     ASYDKYINYKKMVNAKEAVAGFLWATEIVERPQLEKMKK"
     gene            11183..12802
                     /locus_tag="PLE88_01840"
     CDS             11183..12802
                     /locus_tag="PLE88_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOM75239.1"
                     /translation="MNRLFQMLAWTVAAGIFASSVSANTDSAGTARADSRMSEIPIQK
                     GKAAWNVCDFGAAGDGKALDSPAIDKAIEACAQAGGGTVYLPAGTYLSGSIHLKSNVN
                     LHLGAGAVILGAPQEMNAYDFTEPWEGTAYQDGGHTYFHNSLIWGENLTNVSITGYGM
                     INGGGLVRSDGLLDRMCGFSTWNKPEAASAGREMQPVRLGNKAIALKLCRNVLMRDIT
                     IYHGGHFAILATGCDGLTIDNVTIDTNRDGIDIDCCRNTTVSNCRINAPGDDALCPKS
                     SFALKKNVITENLTIVNCQVSGFEEGTLLDGTMKPSRVKNGRIKFGTEANGGFRNVTV
                     SNCTFRGCRGLALEEVDGGIMENIVINNIAMMDVAAYPIYITTGKRNRGPNVTEPSRM
                     RNILISNVIATGIEPTSGIQITGLPEQPIEGVRLDNIWLVFNGGGTKEDAARIPPELG
                     TGYPEPRNIGVMPAYGVFARHVRDLELQNIKISFLKEDLRPAMVCVDVDGLEIDSFKA
                     QLAAEVPAARFEDVRRLIIRNSPVLDKVSVK"
     gene            13132..14769
                     /locus_tag="PLE88_01845"
     CDS             13132..14769
                     /locus_tag="PLE88_01845"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOM75240.1"
                     /translation="MLLNTKVFVKTLLSVGILSVWTASVYGTDAALPAPKAQPEIRTP
                     KAPAEPRINGPSIFGVRPGHPFLYRIPATGNYPMEFAADKLPEGLTIDGKTGQITGTL
                     AAPGQYEVILRAKNSLGVSEKKFKIVAGETIALTPPLGWNSWNCWGPTVDAEKVLAAA
                     KAMVESDLINHGWTYINIDDAWQGKRGGPFNAIQGNEKFPDMKSLCDAIHAMGLKVGI
                     YSTPWTTSYAEYIGGSSENPDGSWEKPTIPKSGNVNKKRLPWAIGKYSFAVNDAKQWA
                     AWGIDYLKYDWNPNEEPETREMYDALRSSGRDIILSLSNSMPFENVPVLSKIANCWRT
                     TGDIRDNWWSVTGIGFTQTRWAPYQSQGHWNDPDMLVVGYVGGWGGRQPKPSGLSPDE
                     QYTHITLWSLLASPLLIGCDMTRLDEFTLNLLTNDEVLAVNQDALGKQAVQIVETGQP
                     VTIVRSDRPEQKRTLKPYQIWARPLEDGSIAVGLFNLSDQPAPITASWKDLKLTGRQT
                     VRDLWRQKELGQYDEQFQMVVAPHGAELVKIKSVVLP"
     gene            14818..15837
                     /locus_tag="PLE88_01850"
     CDS             14818..15837
                     /locus_tag="PLE88_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM75241.1"
                     /translation="MKCRFVQDIAGLVLILMTAGVGHAEHRASWMQKAKWGVMNHYLA
                     DWIAKTDNIPMSVERWNQLVDNFDVEGLAEQVKSVGAGYYIITIGQNSGYYLAPNPVY
                     DQLTGIQPSKCSRRDLVSDLSAALTRRGIRLIVYLPAGAPSRDRLACQTLQWKDGADR
                     NLEFQLRWEKIIRDWSIRWGDKVSGWWFDGCYWPNTMYRYEQPPNFASFAAAARAGNS
                     ASIVAFNPGVVDRIISVTPHEDYSAGEINEPERLMIRRSVDGLMDGSQVHVLSYLGQT
                     WGMGQPRFSAEQVSEWSRRIVQYQGAVTWDVPIQPSGLMAQVFMDQLKVIGAAVAAVN
                     PEISR"
     gene            16208..18193
                     /locus_tag="PLE88_01855"
     CDS             16208..18193
                     /locus_tag="PLE88_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOM75242.1"
                     /translation="MQAINRKYAFAAIILYAAALTFAQAERQQPRQQRTNRSTTRRTQ
                     RVIPMKEVRLVSPDGKVSFIIDSNPERLTWSAMIESTIVIEPSPLDMRIDGYDISSGV
                     IFNALQEYSIDEAYPWHGAHSRAVNRCNGARITYTHDLSQIPYILEVRVFNDGVAYRH
                     IIDGNAEAVRTPNEYSRFILPAGTTVWYHDLDGHYEAEYTKQDISDVKAGQWAGPPLT
                     FQLPDGAGYGSITEANLVNYSGMALENDGRRGWIIGLGHRQPVNYPYELRYGRDEAKR
                     LAVPAAISGSITTPWRVVFIARDLNVLVNSDILPNLCPPADPQYFPQGMDTPWVEPGL
                     CVWDYVDRNYVRNEDADQFQRMKDFSRMAGQIGAKYHILEGFAYGWSDEQIREFTDYS
                     KQQGVRVLFWRHSRNLRTPEQREEFFARLNRLGVAGAKIDFFDHEAKENIDLYEALLE
                     KAAHYQCVIDFHGANKPTGRLRTWPNAMIYEAVRGMESSSLQQRARHETILPFTRYLA
                     GPCDYTTMVFNDRRRDSTWAHQIACLATFHSPILTIAAHPQSVLDNPALEVIKSIKPV
                     WDETIVLPDSRIGELSIFARRSGDMWMLAITAAGPSRTLQVPLSFLSEGAYKAIYVRD
                     NMNKPDAVVVEKSTASKNEILTIQLADGGGFIGQFVK"
     gene            complement(18201..19388)
                     /locus_tag="PLE88_01860"
     CDS             complement(18201..19388)
                     /locus_tag="PLE88_01860"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOM75243.1"
                     /translation="MLTETQLTTCSTNSAAPVNVLFAGERAISDDLLFLLGSRGYHWN
                     RIRLDELYTYAQQGTICGPVIVDAEMIDSDSVSAYQSLFEKLDQQDTPVILYRCPSCI
                     KTDHLQLAVRAESLGYEELWARIESSVQFSKRLQKLKGQKENYLLAKDTAQQLEMAGR
                     VQRNFLPAQLPDNDTIHWAAVFRPAEWVSGDIYDVARLDEDHIGFYLADAVGHSMPAA
                     LLTMFLKHSIIMRQTFGNDYTIFKPQEVITALNLRMTQQELSGCLFATCFYGLINIRT
                     LTLEYARAGHPYPLLIRGGQIHQLESRGGLLGVFEEAQFEQKTLQLHPGDKLFIYSDG
                     GEPLIAHAADEGQLQFKQAFVDICNLPIVPMLQAFNRMAIHHDFPPNQIDDITAIGMH
                     VVK"
     gene            complement(19543..20262)
                     /locus_tag="PLE88_01865"
     CDS             complement(19543..20262)
                     /locus_tag="PLE88_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOM75244.1"
                     /translation="MGVIADKLKKLQDEIASVCARCRRSEKTIQTIVVTKTASIEAIH
                     EVIRAGAVELGENRVQHLKQISDEVDAFLKEKAEAEGLPRCVNWHMIGHLQRNKVRQT
                     LQICRIIHSVDTLRLAEEINIQAQKMGIHINVLMQVNCSGEPQKYGVPVGAAVHLAEQ
                     IDTMPNLRLIGLMTMAPLTMDKDIVRSCFARARELFEEIRAEHIGGAKFKHLSMGMSQ
                     DYPIAIEEGATMLRIGSAIFS"
     gene            complement(20287..21381)
                     /locus_tag="PLE88_01870"
     CDS             complement(20287..21381)
                     /locus_tag="PLE88_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOM75245.1"
                     /translation="MGKNKKNTAAELITDNARQVYNSILANRKPTMSTPIRSLSNVKY
                     HVKKGHFEIKGNTKNRTLTVNTVKTFAQTLKMMALSKELIDNDNMATKREAYYISKNW
                     GKAGFDEQPESDTVMDDMEAMFGVNREQLRFVPEEKGGDVAGQLIVLDKDAGGKPLKI
                     DCTRFGSGAYSIPINVEELGFQSKAKFILAIETAGAFQRLVKCDYWKKADCILISMGG
                     VPTRACRRFIRRLSDTLKIPVYAFVDGDPYGYFNIYRTLKVGSGNSAHLNEFFCVPQA
                     RFLGVTPQDIIDYKLPTHPLKDVDVKRAKDALKNDPFVAHHKPWQEAINQMLKMGVRV
                     EQQAFAKHGLDFVVDHYLPDKLKNPNKFLP"
     gene            complement(21805..22212)
                     /locus_tag="PLE88_01875"
     CDS             complement(21805..22212)
                     /locus_tag="PLE88_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOM75246.1"
                     /translation="MKVVQDSFFVKTHKRNEMIDITSEVEAAITRSGIDTGILTVFCP
                     HTTAAVTINENADPDVVHDILMTLEELVPHFRQGYQHSEGNSDAHVKSSLVGVSKTVL
                     IQNGRLKLGTWQALFFCEFDGPRQRSVYLSICG"
     gene            complement(22223..23986)
                     /gene="argS"
                     /locus_tag="PLE88_01880"
     CDS             complement(22223..23986)
                     /gene="argS"
                     /locus_tag="PLE88_01880"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOM75247.1"
                     /translation="MKRLTDILEERISGAIRQVTGRSFPAMVRPAAEPKYGDYQANGI
                     MPAAKQIKTNPRQLAQQVAAVLDIADICQPPEIAGPGFINLRLKDEFLAARLIEINSN
                     KQRLGIEKTKKTKTVVVDYSGPNIAKQMHVGHLRSTIIGDCIARLYEFQGHTVIRQNH
                     IGDWGTQFGMLIALLKEQYPHILDNLSSAHIADLETFYQDAKRKSEEDKTFEQKARQE
                     VVRLHNHDADTILVWKQIVQESRKHYLPIYKSLNIMLTADDERGESFYADKLASVVEE
                     FKGRRLASESDGAICIFPEGFKNKEGQPLPFIIQKSDGAFLYATTDLAALRYRIQELH
                     ADTIIYVTDARQSLHFQMLFAAAKMAGWTDSGTELVHVTFGTMLGEDGKPFKTRSGGT
                     VKLKDLLDEAVERARKIVEEKNPSLSAEQKEHIAQAVGIGAVKYADYSNNRNSDYVFS
                     FDKMLAMDGNTAPYMQYAYARIKSIERKASGREVDIKRELSGLCSLSFTSEAEKELAK
                     LLIRYDQAIDAAAQECRPNYLTGYLYDLSQAFSRFYAACPVLEADAQQRPLRLLLCDL
                     TARTIRHGMSELLGIEVVEHM"
     gene            complement(23986..24411)
                     /gene="rsfS"
                     /locus_tag="PLE88_01885"
     CDS             complement(23986..24411)
                     /gene="rsfS"
                     /locus_tag="PLE88_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOM75248.1"
                     /translation="MAKKTSKKTVKKQTAKINIKRPKRSTDKQLALSAARIAQDRNCT
                     DITILDLRGISPAANYFVIATGTSDRQSRTVADEISQEAKKQGHQRFGIAGYERGQWI
                     LLDFVTVVVHIFDAEHRNYYNLEMLWGDAKQLKIDKKQE"
     gene            complement(24507..26891)
                     /locus_tag="PLE88_01890"
     CDS             complement(24507..26891)
                     /locus_tag="PLE88_01890"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOM75249.1"
                     /translation="MMVLSSTAMAQEQAAQTATVSAKFAPMAPYLAMGSAVISLLMAI
                     YFYKKVMQAPAGSPKMMEIADHVREGAYAYLFRQYKVVTVVFIILVAVFAFLAYKGVQ
                     NPFVPVAFLTGGFFSGLCGFLGMKTATNASSRTAQGASESLNRGLQVAFRSGAVMGLV
                     VVGFGLLDISLWYILLDKVFFALGPDGLTKGFKIGSFVLVHPGIDSAHKLVEITTTMI
                     TFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVG
                     DVAGMGADLYESYCGSILSTAALGAALPAASLAAKGIDPIMAVLSPMIVAGIGILLSI
                     AGIFMVKCKEGASQKNLLTALLKGTLSSSILIVIALAIMAKMGMITWGIFWSVISGLA
                     AGVIIGQITEYYTSDEYNPTKGIAGQAVMGPATTIIDGLAVGMFSTGGSVITIVIGII
                     CAYAFAGGFSDVSMGLYGIGFAAVGMLSTLGITLATDAYGPIADNAGGNAEMSGLGEE
                     VRKRTDALDALGNTTAATGKGFAIGSAALTAMALLAAYIEEVRIWIGRLANESPEGIY
                     MGFTSAQVKVASISQFVEVYDLTIMNPKLICGLFIGAMMAFIFCAMTMKAVGRAAGAM
                     VNEVRRQFREIPGIMEGKGKPDYARCVAISTAGAQKEMVVPSALAIVVPVATGLLLGV
                     AGVMGLLAGGLTAGFVLAITLNNAGGAWDNAKKYIEKGAYGGKKLPDGTKNPVHGAAV
                     VGDTVGDPFKDTSGPSLNILIKLMTMVSVVFSGVVVKFAPMIGQWFFNLFGLSS"
     gene            complement(27085..28287)
                     /gene="argJ"
                     /locus_tag="PLE88_01895"
     CDS             complement(27085..28287)
                     /gene="argJ"
                     /locus_tag="PLE88_01895"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOM75250.1"
                     /translation="MTNISITAPKGFLAAGVHCGIKQSGKPDLAIVYCPTGAAAAAMF
                     TTNAIQAASVAVCRQHIASKKIYAAVINSGNANACTGTKGLAAAEAICRKTASQLQIA
                     PQQVLIASTGIIGHQLPLSKITSGIASAASQLRADSAGGLAFAKAIMTTDTRCKQAMR
                     TVKIGGKTIAIAGTAKGAGMIGPNMATTISVITTDAAITKILLDKALRNAVGLSLNRL
                     TVDGHQSTNDTAMILASGLAENKTIKKQDIGYRRFSAALADLCSDLAKQMALDAEGAT
                     RMFKVEIKEAATKEDAFKAARAVADYDLVKCAIHGGDPNWGRIICAVGSCGVKLNPNR
                     LSCKIGDIYVFRNGKPTNFDSKKVSKIISRKEHTITVYLGSGRHNDFCYGCDLSRDYV
                     TINADYHT"
     gene            complement(28493..30838)
                     /locus_tag="PLE88_01900"
     CDS             complement(28493..30838)
                     /locus_tag="PLE88_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75251.1"
                     /translation="MKKVLKILLFLLICASLSAGYNYSVNGKVFYSGGTDSVKGATVT
                     LTVNWAVGGVASGSTTTEKDGSFSFVVSSDSLDYIGATMELKATTAFCSGSTTWICKS
                     YSEGKDVWIFCNVQINPDLSTVVKPRPFISAGRQLPVTTVAYLDQPVHPVLVQQFQVA
                     LRWNPVHMNIAAVESSPGSPFIVETWGPGPDIGTAIVKGHSLFGPVPMKIWPEPPDSF
                     FDIFFDVLVPPEEMPLYSTVTVVPLETYLYGPEVYYPYQHQSDFLIGEPEPCKAVFMI
                     DSFEEWETALHSSLPDANIRPATLTFWRQYMDYWANPTSEIEGEPYPRSEFIPPIEPE
                     GMLYVYEGGGGGGGIPEDAGLVMAWGTTPPAEEGNYASAWRWDYGLDPDLSNCTIQVT
                     VSPMTPNINIVSFSIVDIANRMRTWWWNVPGTIPLGVPTTVKINTALTGVAAASPVAT
                     GYMNVPGFDITKSQFFDVDENFKYIFGQQPVPPPGQQIFVYGWNYWHNLLVTKNTQAY
                     KGTWPKYSQPPVVLNPEQAVPNILGWDEVSIYKPPLWPIMADDWICLDGRPVTDIHWW
                     GSFKGWTQPYLPRVVPKAFHIGIWTDVPAGPNNPYSHPGKLIWEHICDNWVWNFAGYD
                     IDPRCEYPEMPCFRNEACFQFNQLLSEDDWFYQKPSENPEQPNIYWLSIAAIYDDNAL
                     IANPWGWKTRPHLFNDDAVRIIATAEGLWPPQVGSVFGKGIPIQIPEYPAPGASWDLA
                     FELTTNQKPPCHTLQGDINHDCVVNLHDFAILASDWLLTSP"
     gene            31293..33689
                     /locus_tag="PLE88_01905"
     CDS             31293..33689
                     /locus_tag="PLE88_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOM75252.1"
                     /translation="MSIIIFFIFSGYIQSQSLSELPQFSQKTAFDGQIEGFAPAPAEP
                     LSLWYRRPAEKWTEALAIGNGRLGAMVFGGIVNERLQLNEDTLWAGGPYEPINPEALA
                     ALPEARRLIFEGQYRQASRFIDSKMMGKPLTQMPYQTVGDVLLQFDSFERVADYRRDL
                     NLDTAVATTSFTADGVRYTRQVFSSSVDQVIAVHLTADKPGHISCRIGMKSPQKASVK
                     CDGSDTLVLSGVNGGSAGIAGALKFQARLLAKTTGGTLSPEQDCLKIIGADSAVLLIV
                     IATSYKKYNDVSCDPEFLTKQAIEAAGKRSVQDILKDHIAAHQSLFRRVSLDLGSTEA
                     IKNPTDRRIADFAKGNDPQLAALYFQFGRYLLICSSRPGTQPANLQGIWNDSMTPPWG
                     SKYTININTEMNYWPAEPTNLGECVEPLIAMVMDLSQTGRRIAQMHWGAGGWVCHHNT
                     DLWRAAAPIDGATWGFWPTGGAWLCKHLWEHYEYNGDRIYLEKIWPVLKEAAQFFLDT
                     LVEESTHKWLVTCPSLSPENGHQFGASICAGPTMDSQIIRDLFDSCIIAGGILNTDQS
                     FRSQLVKVRDRLAPNQIGNAGQLQEWLEDWDMEAPDKHHRHVSHLYGLYPSDQINIYD
                     TPELAAAARKSLEIRGDNATGWGIGWRLNLWARLLDAEHAYSILKLLIAPERTYPNMF
                     DAHPPFQIDGNFGGTAGIAEMMMHSRVQYSGESMLTEIELLPALPKIWPNGRIQGLRA
                     KGGFELDIEWREGRLNYAKIRSLLGNLCRVRYAGRCYPIQIQKGQEIVWQVPPIADGK
                     "
     gene            33874..35217
                     /locus_tag="PLE88_01910"
     CDS             33874..35217
                     /locus_tag="PLE88_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOM75253.1"
                     /translation="MSNRVFFGILLLAATNLWGDNAADWMTWRGPNMMGVSPDGNPPI
                     EWSETKNIVWKVRLLGDESNSTPVIVGDRIFFQTAVKTDKKAESAQQQTAVQPADQRR
                     GPGRGKPTNIYRFNVVCLDRTSGRQIWETTVCEMLPHEGHHNDHGFASYSPITDGKLL
                     WANFGSRGIYCLDFDGNMKWSRHFGPMTIRSGFGEGGSAALAGDGLFVVRDHEGDSCI
                     LALNKLTGETIWQKQRDEKTSWTTPIAAEVGGKQQIVVAGANRTRAYDALSGQVIWEC
                     GGQTQNVIPTPVLGFGRIYCTSGFRGSMMQAIELGRTGDLTGTDAVAWQITEATPYVP
                     SPLLYGDKLYFCSGINPILSCVNAQTGAPYYLKKELEQMKGIYASPVGAAGRVYLIGR
                     NGVCYVLKNSDTLEVLAVNTLDDRFDCSPVVVGNRLYLKGKQYLYCIADAAKQTP"
     gene            35248..35841
                     /locus_tag="PLE88_01915"
     CDS             35248..35841
                     /locus_tag="PLE88_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOM75254.1"
                     /translation="MSPASGSQHRESISQDIPGLGQVLFVPSKRAKRMSISIRPGCPV
                     RIAYPRCTTLMQAVAFLEKHRDWAQKHVARVKQMERMHRRQAGMPAFCGDEAGAAQWL
                     CRRLQMLADRYGFKFSRVSIRRQKTRWGSCSAKNSISLNIRLAHLTPQLCDYVLLHEL
                     VHTKVKNHSKQFWRCLDVCCAGQARQLDKQLKQYLYR"
     gene            35903..36574
                     /locus_tag="PLE88_01920"
     CDS             35903..36574
                     /locus_tag="PLE88_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOM75255.1"
                     /translation="MNSLQLPRGVVLDMDGVLTDSEPFICRAACLMFAEHGLSVEPDD
                     FLPFVGTGENRYLGGVAEKYNFPIDIEQAKKRTYDIYLEIIKGNLKPLPGVQTFLRRC
                     RSMGKKLAIASSADRRKVRGNLIEIGLPEEWFDAVVVGEDVSRKKPDPMIFQTAIERL
                     GLNPSECLVVEDAVSGVEAAKRAGARCLALTTSFPAEKLRAADWIAADLANVWPHILS
                     WPDGL"
     gene            complement(36674..37645)
                     /locus_tag="PLE88_01925"
     CDS             complement(36674..37645)
                     /locus_tag="PLE88_01925"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HOM75256.1"
                     /translation="MKTADELKAFYDSRLRSDMQQLQQQRRQLVQKSMLAAGILAATG
                     LVIGGIAVWQGAEPIVLVLFLIGGLIGGGIFFAAAGRKYKQAFKQKIIGGLVRFIEPG
                     LDYQPEGFIDQQTFEAAGLFHQHIDRYGGEDLVTGIVGKTQMAFSEVHAEYKTTTRTS
                     KGHTQTHWHTIFKGLFFAADFNKHFRGRTIVLPDTAEKLFGGLGRMLQSWNIARADLV
                     KLEDPEFEREFAVYGDDQIESRYILSTSLMQRILDFQKKAGVKISLSFVGSTMYAAVP
                     LARDLFEPPYFSTPDSIDRIEDYYRDLMLAVGIVDDLNLNTRIWSKE"
     gene            complement(37642..38217)
                     /locus_tag="PLE88_01930"
     CDS             complement(37642..38217)
                     /locus_tag="PLE88_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOM75257.1"
                     /translation="MSGIIIFVIAGGIILLIPVLMYNMLVGRRNQVHNILGTMDALLK
                     KRWDLIPNLAATVKGYADHERALFESVANARSKAQAGLLNDEEKVELENAFTRMVSVV
                     RAVAENYPQLKAGENFLHLQRTLNELEEQISAARRAYNVSVTDYNNAVQMFPTNLLAG
                     LFGFTEKPLFQIDQDQRQVPSAGAILGGHKA"
     gene            38612..39694
                     /locus_tag="PLE88_01935"
     CDS             38612..39694
                     /locus_tag="PLE88_01935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOM75258.1"
                     /translation="MSTEEKPKLNRNGSGCCRQNCQLEAITPLAKKINCLDVKRIADI
                     CIQEIPKLIGAKLASLYLLDENSDILHLEKNNHPFLINNIVSLNQNPPSPMVAAVRSK
                     ELLIIDNVDSHKTPHIHNTQRIFAKNYQTKSCIIAPLICHGRVVGVLNLSDKDDKGPF
                     TEDDIAVVELFRQLIGASIGNISLFEKTQRQAKTDGLTGMLNHRMFYEMLEIELRRAQ
                     RYGGKLSIIMIDIDNLKPINDNYGHRAGDMAIKQIARRISVCIRQIDIAARYGGDEFA
                     VILPNTALSDAAVVAERMVAVTTGTPMLWEQHRIPLSISVGVGQYDGENCAGDVTKTT
                     DQALYAAKQAGKGRVSVFEPSKTAAR"
     gene            complement(39736..40224)
                     /gene="tadA"
                     /locus_tag="PLE88_01940"
     CDS             complement(39736..40224)
                     /gene="tadA"
                     /locus_tag="PLE88_01940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOM75259.1"
                     /translation="MTEPSIDLQSDQRYMRMAIDQAYIAEENGDVPIGCVIVYQNRVI
                     AKGYNQRELLSDPTAHAEIIALTQAADYISSWRLTGCTIYVTLEPCPMCAGALVLGRL
                     DRLVYGTDDPKTGAVKSLYNIVQDPRLNHRLEVTSGILQEECQKQLQAFFQRRRQENA
                     RQ"
     gene            40794..41960
                     /locus_tag="PLE88_01945"
     CDS             40794..41960
                     /locus_tag="PLE88_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOM75260.1"
                     /translation="MLSKKVVWGNGFLCLAAVLCLSPFAAALDVCGQGFVFIDGSEDP
                     VVDINCETGYTFVYNGATVNLREGAHISENLGGSGGGILTSGGNITINIYAGTIDTML
                     NTTSTDIVTIYGSSFVLGGDQLSAGQTQIVGPLGTDGNPMLLTGDGTEGPISIPLVLE
                     EGAIVNLNISQTAPDIYVQPAQGIWDFGNVELGQSSTVVVEIYNHGNADLIVYDVQLT
                     GDEAFTIDGVFPTQDNPLIIAPNTSKGVDFEVTFAPASQGYKSATVTIISNDEDEGIV
                     EVSLYGMGIAAEVPPLVQIQNILAYFDASAADGTLLGYGPGNSPDKRLNALRNMIEAA
                     DDLIQDGAYDLAIIQLEAIAKKTDGIAKPQDFAVGEAVPVLNAMINDLMAALAS"
     gene            complement(42016..42088)
                     /locus_tag="PLE88_01950"
     tRNA            complement(42016..42088)
                     /locus_tag="PLE88_01950"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42052..42054),aa:Glu,seq:ctc)
     gene            complement(42136..42209)
                     /locus_tag="PLE88_01955"
     tRNA            complement(42136..42209)
                     /locus_tag="PLE88_01955"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42173..42175),aa:Met,seq:cat)
     gene            complement(42585..43799)
                     /locus_tag="PLE88_01960"
     CDS             complement(42585..43799)
                     /locus_tag="PLE88_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOM75261.1"
                     /translation="MKKQIGTILLVLVICAGGSGCSRQFIQNSRHWLGTWAASAQPVE
                     AELMPPDYQTLDNTTIRQVIRVSAGGRHLRVRFSNSFADASDTLHIERAFIAESAGAG
                     VIAADTLTPLTFGGKPSIAVPAGAVIASDVAAFHLKPNSDLAVSLYISKAPAKTTGHR
                     SARGEAAFIQAGNAADAASLPQNTASKCWYWLCGLDVLAPSSCGAVVCLGDSLTDGKG
                     STEGANRRWPNYLAQRLQADSTTACIGVLNQGIGGNCLLAGGIGPTALERLDRDVLDQ
                     PAARWIIVLEGINDLGTGTADARQIIAGYEQIIERSRARGLRVYGATLLPCGRSFYDN
                     PQLQAQRQIINHWIRTSGAFDAVIDFDAALHNPANPQLLLPEADSGDHLHLNDRGYEM
                     MAEAVDLTLFKQ"
     gene            43976..44818
                     /locus_tag="PLE88_01965"
     CDS             43976..44818
                     /locus_tag="PLE88_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75262.1"
                     /translation="MKDRMFILLLLGFWAGCAPQPMKPQQPLCAGKSSIQEAAAALAL
                     QRQNLQPFRASADCTISWIDQQGKKRDEPIRSAAIAFVPDSRVDFIGQLLFKEARFGA
                     NESEFWLRIKAELDTYWWGTRAQAAQCRQGLIVNPDSLVEALGIVAVGPDWKLFHRDG
                     FDIVSLYESGRIIKRAYINACDYRVEQIEYFDDEQIRRVQVQLGEYAAGETGIVAPTR
                     IRAASFDRMGQEESAVLIRLKNIRPLPPEQIGRKLFVRPGRDGYANVYRLDENCEFAQ
                     EEGQ"
     gene            44815..45714
                     /locus_tag="PLE88_01970"
     CDS             44815..45714
                     /locus_tag="PLE88_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOM75263.1"
                     /translation="MTRQTLTSRLGQGLFFVDGAMGTQLIEQGARAGGCNDMLNLDSP
                     ALVRAVHQRYLDAGVDAVITNTFGANALSLGRYGLADKTYAVNRAGAELARQTAGDDR
                     YVLGDIGPCGDFLEPLGTITGQQLLAAFARQAEGLRDGGVDGFIIETMTALEEVRTAI
                     AAVRTVSDDLPILVSLAYDAAADGRARTMMGITPAQAVEYLSPLGIAGLGFNCGTLDM
                     AGYVKLAGDYAAALKGTGLLLLAEPNAGRPHLEGDKAVYTLSPDAFALALVQIRQAGA
                     SILGGCCGTSPAHLSAAVQKLTR"
     gene            45899..46288
                     /locus_tag="PLE88_01975"
     CDS             45899..46288
                     /locus_tag="PLE88_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75264.1"
                     /translation="MAKKLYVGNLDYSIGTNTITEWFSAYGTVESVNLVKDRQTDQSK
                     GYGFVEMSTDEEASAAIEGLNDTEHLGRNIKVAAANPPKTKAPRRAGGFSSGRRFSGQ
                     GGPRPGGRPGGQRRFGGGDRGGRDSRH"
     gene            46775..49087
                     /gene="hypF"
                     /locus_tag="PLE88_01980"
     CDS             46775..49087
                     /gene="hypF"
                     /locus_tag="PLE88_01980"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOM75265.1"
                     /translation="MIRERITICGRVQGVGCRPFVFRLAQEAGLCGYVRNDTQGVFIE
                     VQGSKAAIEQFKSQLLDCQNPAYPPLLKIVSLTVCPLDPVSGENGFSIADSDPAGTPA
                     SQVTPDCAVCKACLNEMNNPADRRYRYPFINCTHCGPRYSIVRAIPYDRANTTMDAFS
                     MCSVCRRQYADVLDRRFHAQPVACPVCGPAIRLTDADGNTIEADSDKAIARCAEILLR
                     GGIAAIKGLGGYHLAADAHNERAVSELRRRKRRDAKPFALMAASVETVSCYAIVDPLS
                     RQLLESPQAPIVLLDKIQPNALAPSVANGLHRLGFMLCYTPLHYLLFAQPGIAVLVMT
                     SANLSDEPLICKDQQALDELGGIADVFLMHNREIYRPIDDSVLHTVNNQPAFLRRARG
                     YVPAPVFRSKPASKDIFAAGGDLKNTICFVKGNQYLVSEHIGDLADAKVFRHYVRSIE
                     HLQQLFDVRPQAAVCDLHPGYLSSQFAQQAGIPLLRVQHHWAHIASVLADCNEQGPVI
                     GLAADGTGYGTDGAVWGCECLIASLTEFERFGHLAYYPLAGGDKAAQEAIRPLVGLLA
                     PDGNFGCLTEYMDILNRIEPDVEKIHLICLQIQKRLNTVATSSLGRLFDAAAALAGAG
                     AVNRYEAQLPMHLEAMTARGAEEGYPVQLDSEGAQICLSPAPIIKAMIKDIRDGMDAS
                     VIAARFQNAVCEGLLQMAFCARQKRQLNTVALSGGVFCNRYLANRLIVRLQQEQFRVL
                     WKQSVPVSDGGISLGQAAIAAEMLDKGKGW"
     gene            49092..49349
                     /locus_tag="PLE88_01985"
     CDS             49092..49349
                     /locus_tag="PLE88_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOM75266.1"
                     /translation="MCLAVPARIIEKNGDRAVADAMGNRWTIRIALTPDIEVGDIVLI
                     HAGYAISKVDPQEARKTWELFEEIARYEAQQNTAHPKETYQ"
     gene            49349..50404
                     /gene="hypD"
                     /locus_tag="PLE88_01990"
     CDS             49349..50404
                     /gene="hypD"
                     /locus_tag="PLE88_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOM75267.1"
                     /translation="MLERIEKTCQTLHSACSRLSRQINIMEVCGTHTVAIFRSGIRSL
                     LPDNLRLLSGPGCPVCVTDQSYIDTLLQIAQRTDVLIATYGDMMRVPGSTGSMESLPN
                     VHVVLSADDALMLAQKNPDKIVVFAAVGFETTAPATAVVVQDAAREGIDNFCILCGHK
                     RVIPAMKTLVADRNDKIDAFLCPGHVSVILGSDAYIPIAQVFCRPCVAAGFEPMQILE
                     GLAAICRQKVEGKASVESVYGAVVTPQGNKTAQQLLADVFEFSDDPWRGLGVIKDGSF
                     KLRKAFKQFDAAERFAIEQIPAPEAGACRCGQVLCGLIDPPQCPLFGRRCTPDAPVGP
                     CMVSTEGACAAWYKYGQ"
     gene            50439..51458
                     /gene="hypE"
                     /locus_tag="PLE88_01995"
     CDS             50439..51458
                     /gene="hypE"
                     /locus_tag="PLE88_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOM75268.1"
                     /translation="MNEHKTIMLAHGGGGRLMEELISRTIVPRLGSGQSVQLTDSALL
                     DVPAGKLCFTTDSFVVKPLFFSGGDIGKLAVCGTVNDLAVAGARPMALSLSLILEEGL
                     AMDTLEAVLDSIAQTARAAGVQIITGDTKVVEKGAADQIFINTAGIGMRLQNAKPGYE
                     HIQPGDVILLNGPLGSHGLTILLQRQGLEFQTMLASDCACLHELTAMLYENIGRSVKF
                     MRDPTRGGAAAVLNEIARAASVCIEIKETDLPADKTVQAAADMLGFDILNVANEGKVL
                     AVVAPEAAETALALWRSHPLGRDAAIIGTITRSEDKALVELITRIGGRRIVPTPYGRE
                     LPRIC"
     gene            51458..51820
                     /locus_tag="PLE88_02000"
     CDS             51458..51820
                     /locus_tag="PLE88_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOM75269.1"
                     /translation="MHETAIAESIVRTILENARQLQAKPLSAQISCGQFNTVNDELMQ
                     TAFQAAAAGTLCEGMTLTIRHIPLQACCRQCKTVFEFDVYKPVCTACGGDKYDFKPDA
                     PLLLETIEFEDNAGFAKG"
     gene            51849..52538
                     /gene="hypB"
                     /locus_tag="PLE88_02005"
     CDS             51849..52538
                     /gene="hypB"
                     /locus_tag="PLE88_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOM75270.1"
                     /translation="MKISLNQRILSRNEECARKNKVFFDEYQLFCLNIISSPGAGKTT
                     LLTRTITDLKGRLSVGVIEGDIKTDTDARKIRAAGAQAVQIETQGACHLSAEQVHNAL
                     TLLDVKTLDVIIIENVGNLVCPSAFDLGEHRRAVVLSIPEGDDKPVKYPGTFTGADVI
                     LINKIDLADYIPFDLEKVLADIHMLNPHAAVFQVSAATGQGMNAWYDWLLAECRKHDG
                     RQSESIEKPSR"
     gene            complement(52505..53827)
                     /locus_tag="PLE88_02010"
     CDS             complement(52505..53827)
                     /locus_tag="PLE88_02010"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM75271.1"
                     /translation="MLKQSALSIGAFTIVPRSVLGGPNQTPPSEKLNIACIGVGGKGY
                     SDAQSVSSQNIVALCDVDDNYAAKTYNQFPHAKRYRDYRVMLEKEKNIDAVVVATPDH
                     THALISLTAMRLGKHVYCQKPLTHTVEEAYRMSRTADQMKVATQMGNQGQADEGPRRI
                     QEMIADGAIGAVQEVHVWTDRPMNGLFDTYWPQGIERPAEAADIPAGLDWDLWLGPAP
                     HRPYHPCYHPFRWRGWWDFGTGALGDIGCHALDCVFRALKLRYPLSVQASCTQVNRET
                     YPVASMVTYEFGAREDMPPVTVTWYDGGLKPPRPEELEPGRQLGIGGCLYIGQKGKIL
                     DGRIIPESKMQEYTPPPKTLPRSIGHYEEWIAACKGGTPAGSNFGFAGLLTEVVLLGN
                     IALRMELRESLCRQKLLWDAEKREFTNLPQANAFLKKTYRDGFSIDSL"
BASE COUNT        13439 a        13203 c        13911 g        13556 t
ORIGIN      
        1 ggcaatgtgc gctaccacaa cggcacaccg ggccgacagt ttactgaaca aatcctggcc
       61 tataatgctg ttactatcga ccgcagcgat atggccagtc cctcagccag cacctatggc
      121 aacggcgatt tgacattgta cgagccgggg caggacggtc tggctgtgac ggaaattgac
      181 ggccagaggg cctaccataa caaggcctcg cgctaccagc gcatcatgct gctcaatacg
      241 atggatttga cacgtcccta tgcggtagat atattcaggg tgacgggggg cacaacacac
      301 gattatgtct ggcacggttc aattcgatat gaccaaactg cccaatgcag ttttgctttg
      361 acgccgatgc ccggcgagac acccatgctg gaaggatctg aaacatggta tgaaccgacc
      421 agttcgggtt cttcatttcc ctattacggt tttttccgtg acatgagcag caatccagcg
      481 ccgggcaatt ttcagatcag ctatctggat accaaccgct cctcgccgcg tgacaccaaa
      541 ctgtggatga cctctgagcc ggaacaatat accgtctata tgggcggccg cacgcctgtc
      601 ccggcgcgtg tggacagcga acctatcaac tggtgggtca atggtctgtg gcggccgtcg
      661 acaattatcc gcaggcggac tgcctccggt ccgctgcatg acttattcgt cagcgttatt
      721 gaaccgctca acaacggtgt tagtcgaatt gcatccgtcg aacgcattcc gatggctggt
      781 tcgcaactgg aatcagcggg cttgcgtgtt atgtttaccg atgggcgggc ggatacctat
      841 attgtgaacc tgcacaatcc gcaggtggcc ggcgccaata ccggctccgc tacggtgaca
      901 accgccgatg gaagctattc gctgacgggg cgcatcggaa tgcatacaac aggcagcggc
      961 aatgcgcggg tatgggtgat gaacgcaagc agtttccagt accccggcgg ttcgtttatg
     1021 ccgtcgaacc tatattattc cggccagatt acaggccaaa cccgcatcct gaaaggtgac
     1081 agttatgatg cgctgattac cgatacatcg ctgccgctgg gtacggttct gcgcggcaaa
     1141 tacctctcga tgacgtttgg gaccctctcc ggaagcggca caacaggcat cagccagatg
     1201 tttgagattg atcaggttat gcaatctgac ggccaatatc atatccagct tcccagcgac
     1261 cattatctgg atattacgga tgccgtttcc atcagcggca gcggtgctga tatctgggca
     1321 gcggcagacc aatttcatta cgcgtggcaa tcacaaactg gcgatctgac acttaccgca
     1381 cgggtattga gccagacaaa taccaacgct tgggctaaat ccggcgttat gattcgtgaa
     1441 agcagcgccg ccaactcgcg ctatgtcggc ctgtatataa cgccgagtaa tggcgtgagt
     1501 atgcagtacc gcgacgccgc cggcgcaaat gccattgact ttgcccgtac tgccggcctg
     1561 actgccccgc gctggctgcg cttggtacgg tcgggcaata ctttcatcgg ttatcagtcc
     1621 gtcgacggga taaactggat acaggttgga tcttccggtg tcagcatatc tatgccggct
     1681 gccgtcctga cgggtctggt cgtttgttcg cataataaca gcgttctgaa cacgactact
     1741 ttcgataata tcagtgcaga gctgcccttg accagtgcag atattggtgc ggttggcctg
     1801 gctggaagta cggcatacag cgacattacg attgagcaag ttgccccgct gcggacgttt
     1861 aacggggtca gtacatttga gattgcgcta agtgcttcgc gggcggctgc accccccgat
     1921 accccaacag acctgcgcgc cattgccggc aatgggcagg taacgctgag ctggacagcg
     1981 gctgcagcga gttatatcgt tagacggtca gagattagcg gggggcctta cgttatcgtc
     2041 gcaagcccga tgacggccta ttatacggac acagatgttg ttaacggcac gacgtactat
     2101 tatgtggttg cagcggtcaa tgaggcggga caaagttccg attctctgca ggtcagcgca
     2161 acgccgagtg cctcggcgcc aacgggactg gtggcgcgat ttgcgttcga taacggtacg
     2221 gctgccgatt cctccggtta tggaaatcac ggcatccttt atggcggctt gacatcgata
     2281 cacgatgccc agcgaggtaa tgtcctcagc tttgacggtg ttaacggcag ctacatacgg
     2341 gtccccagca gcccctcgct caatgcggtg gccaaacaaa tcacaattgc tatgtggctt
     2401 tataccaaca caacggctgt ccgccaactg attgaaaagg gcggaaccag cggtgcggca
     2461 tggtatactg cgccgtgggg gatccgactg gaaaccaacc gccagctttt gttgaagtgg
     2521 ggcaatgcag attttctgaa atcgaccgct attccgtcca atacgtggac gcatgtggcg
     2581 atgacttacg attcgacgct gggaacaaac aacaccaagt tttatattaa tggacttccg
     2641 gatacgcagg cccactggac aggggcagcg gctcccaaca gctacgatct gttcatcggt
     2701 accgattttt acaacaatac ctcccgctgg gcctatctgg gctgcatgga tgatgtctgc
     2761 atctataatc gggctttaac cccgggtgaa attgctgaac tgacaacagc acccgccgcc
     2821 ccaacgggcc ttgcggtgac cggcggcaac agtcaggtcg atctggcgtg gaatgcgtcc
     2881 ccgggtgcag tcagctatgc ggtcaaacgc tcgactgttc ccggcgggcc ttatacaaca
     2941 attattgaag ggataatgac actccattac agtgacagca ctgttgtaaa cggcacgaga
     3001 tattattatg tcatttctgc ttccaacagt gccggcaatg aaagcaccaa cagtgcagaa
     3061 atgtcggctg tgccgcaccg tccgggtgat ctgacacttg acaatgccgt aaatatggac
     3121 gatatggctg cgttatctgc cggctggcag acgttgtatg agattgaaac catgtttgaa
     3181 atagctgcca actggcttga ttaaaacatg aaaggataca cgatgggaaa aggaatttcg
     3241 atggtttttg tgacgactgt tttttacgct gtattattgt gggccgtccc ggcaatatcg
     3301 caaacagatg ccgatattct ttcaataccg ggaattgaac tgaccgaacg gcaaaagcag
     3361 ccggatttgg cttggtggcg gcagtcaatg caaaatcgtg accagcggct tacttggtgg
     3421 agaaaagcac gttttgggat gtttattcat tggggggtgt attctggacc tggcggggag
     3481 tggaaaggtc aaaaagtcag cggctatgcc gaacacctga tgcgaaaatg caagattccc
     3541 cgtcaggaat atctggaaca ggtcgcccgc aaattcaatc cggtagacta tgatccagcc
     3601 gaatgggtgg cgctggctaa agccgccggc atggggtata ttgttattac tgccaagcac
     3661 cacgacggct ttgcaatgta cgattcggcg gtgtctgatt ttgatatcgt ggatgcaacg
     3721 ccttacggca aggatattct tgcgcgtctg cgggacgaat gccgcaaaca aggtatccaa
     3781 ttcggtgtgt attactccca tgcctttgac tgggagcacc ccaatgctcc cggcaatgac
     3841 tgggaatatc aaaatcccgg cggcgacctt ggtttgtggg gcggtgtgcg ctggtatgac
     3901 cagcatccag aaatggtcga acgtatccgc aaatatgttg atgaaaaatc aattccccaa
     3961 atccgagaat tgattaacaa ttatcatccc tcgattatct ggttcgatac atccagcaag
     4021 ctgccgggtt ctgagcttct ccggattctc aaagccgtac ggcaggaaag taacgacgtt
     4081 atcgtcaaca gccgtgtagg cggcgaaggc ggcaattacg gcgactaccg atccacggca
     4141 gacagacccg ccgaatttgt acccgttcga gagtccgact gggagggtat tcccacgacg
     4201 aatgaatctt atgggtacag taagcatgat aaaagccaca agccggcagc acattttatt
     4261 cagcttcttg ccaaagctgt ttgccggggc ggaaacctga tgatgaatat aggccctatg
     4321 ggcagcggca aatttgcttc cgaagatatt gagattctca acggcatcgc tgagtggatg
     4381 aaatattata ggcatactgt gattgaaaca gatcgttccc ccattccttt gcagaattgg
     4441 ggagcggtga cccgaaaggg caataccctt tatcttcata tatttgattg gcctgaggac
     4501 cagattctgc tgctgggcgg actaaaaaat agggtgcgtt ctgttaacct tctcgataga
     4561 agtgaaaaaa agactctttc ttttgaacgt cttaatccct ttgatattca gatttctctg
     4621 ccccctgcgc cgccggataa agtcgatagt gttgttgaag ttgtttttga aggcactcct
     4681 gatggggccg cttcccgtct tctggattcc cgtgttccaa atcgtcttca tgtctttgac
     4741 agcgatttgc acgaccgcgt ccgctttatg gacggcaaaa caaatcggga ctgtacccaa
     4801 gagtggaatg atccgcgggc tgttatcagc tggaaagcac gtctgataca agaaacagtt
     4861 ttcgaactgc aattgattag cgtcgaaaat actgctggcc agggacgcta tgaattaaaa
     4921 gtggataacc agatcttttc ggaacctgta agcaaagaca gtgccgttac aacggcggga
     4981 cgcataacgc tggcggcggg cgaacatgct atttctattc ggccgcttga tgtcggccaa
     5041 ggcgaattaa cccgtcttcg ccggatggac ctgattccaa ttaaaaatcc tccgctgaaa
     5101 atgtggtact caaaacctgc acagaaatgg gaagaggcct tgcccatagg caatggccgg
     5161 ctgggagcaa tggtatttgg cggttcaaag aaggagcacc tgcagcttaa tgaagataca
     5221 ttgtattccg ggtatccggg ttatcgagat gttaaattga acatttatga aagtctgccg
     5281 aaggttgttg aaatgctgaa tgcccgccgt tttgcggaag cagatgattt tatcacaaaa
     5341 aactggctcg gcgcaggtca ggcctgttat cagccgctgg gggacttata tattgatttt
     5401 gagcatagtg aacaggtttc aaattatagg cgccagctgg atatttcgtc ggcgttatgc
     5461 cggatttcct acacggccga taatatcctg tatcagcggg acatatttgc aagttatccg
     5521 gataatgtca ttgttattcg tctgaccgca gacaaacctt cttctttgaa tttctccata
     5581 cggatggatt cgccgcatcc gactgcagca gtcagggcag atgatgcaag acatctggaa
     5641 atgacgggac aagttcccgg cttggtgctt cggcgtacac tcaaatgggt tgaagaccgg
     5701 aacgaaacgt ggaaatatcc tcaaatctgg gacgaaaacg gccggcgcag agatccggaa
     5761 aaaaccatct tttatgggga cgctataggc ggtgaaggaa tgtttttcca tgctgctgtt
     5821 tcagtgtcat tacgcggggg acaaacgaag gctgctgccg atcgtcttac tgtaaatggt
     5881 gcagatgaag ccgtcattct cttcaatgca gcctccagtt ttaacggctt tgataaaaat
     5941 cctgttcgcc agggtctgga tgcagctgca aaatcaaaag gttttcttag agcggcaggg
     6001 caatacagtt ataaggattt gcttggacgc catacagcgg attacaaggc gttgttcgat
     6061 cgtgtctggc ttgatttggg cggctggaat gagtatgccg agatgccgac ggatcagcgg
     6121 atcgctcaat ttggtcaagt ccgggacccg atgctgataa ccttgtattt tcaatttggg
     6181 cgttatctta tgatttccgg ttcacgaccc ggcactcagc cccttaacct gcagggcata
     6241 tggaatcccc atattgttcc gccgtgggcc agtgcctata ccttgaatat taatactgaa
     6301 atgaattact ggccggtgga catctgcaat ttgtctgaat gttttgagcc gctggctcag
     6361 atgattcagg aattagcagt cgacggccgt aaagtcgcca gcgaagtgta tcatgctgac
     6421 ggctgggtct ctcaccacaa cacctccata tggcgggatg cacagccggt tgataatgtg
     6481 acccgaacct cattttggcc aatgagttcg ggctggcttt gtctgcccct ttatgaccat
     6541 tatttgttta ctggagatga agattatctc cgcagcaggg catatcctct tatgaagggg
     6601 gcctgtgaat tttatctcga ttggatggtt gaagactcac aggggtttct tataacaccg
     6661 gtttctactt ctcctgaaaa tgacttcttc tatacagatg ccgcaggcaa acaacagcga
     6721 gccggcgttt gtaggggaac ggcaatggat atggctatta tccgggagct gtttgccaat
     6781 actattgagg cctcccggcg tcttcaagtg gatgcagaat tccgatcgct tcttcagaac
     6841 aaattgccaa aactgcggcc ttatcagatt ggttctcaag gacagctgct cgagtggcag
     6901 gaggaatttg ccgaacctga gcctgcgcat cgtcatattt cccatctttt tggtctccat
     6961 cccggcactc aaattacccc gctgaagaca cccgaactgg ccaatgcggc acgcaaaaca
     7021 ctgcaattgc ggggcgatgg cggcaccggc tggagcaagg catggaaaat taatttctgg
     7081 gcacggctgg aagacggcga tcacgccttc aaaatgctct gtgaacagat caccaagtca
     7141 acactgacga acttgtttga tacacatcct ccttttcaga ttgacggcaa ctttggcggc
     7201 acggcgggga tagcagaaat gcttcttcag agtcatgcgg ggcaggttgt gcttcttccg
     7261 gctctgccat cggcattatc cagcgggtct gttcgcggat tacgggctcg cgggggtttc
     7321 gaaatagaaa tggattggac ggatggatgc cttacatcgg cccggattaa atccctgctc
     7381 ggcaatccgc ttcatcttcg ataccggagt aaaagcgcgg cgtatcatac ccagagcggc
     7441 cagatgctga cagtgaactc gaaccttgaa gaagtgcaat aaaatcgttt tcttttgaaa
     7501 ggagttgtat caggatgtta aagaaatgtg ttttactatt tgtattgaca gcagtattta
     7561 gcagtgcatg ctttgtactg gctgttcctg aaacggcaaa actgcagatt gaaaatatgc
     7621 atacagctgt tgtctttgac aatcagacca aaaatgcagc tgtctattcc aaagatgctc
     7681 agaaaacata tatttcgtct atccaattta aagaagcatt agccactgca cacagaattg
     7741 atctgaacga cacgggattc ggcaaagggc aggctataga gattgtgacc gtctccggcg
     7801 atcgtcagag gctggctctt tatgataggc ttccgtttgt gtttatgcag agcacaatcc
     7861 ataatgcgtc agctgcaatt aaaacaattc catattatgc accttgcgtg ctcaaacttg
     7921 atctgcaaaa atccccccgt cagctaaagg cacacggcac agccggactt accgcggtgg
     7981 acaagaaatc caacagtgga agctacgcat ttttatccat tgttgatccc gatacccgaa
     8041 agggtgtcgt tgctgcgtgg ctgactaacg agcgcggcag cggcgtcttg ttttcggata
     8101 ttcagggtac atcagtaatt cttgagccgc ggattgacta tgggcgcctg cagctgcaga
     8161 ccggccaaag cgaaactctt gaaaccttgc tggttggctg gtttgatgat gtacgcttgg
     8221 gcctcgaagc ctatgcagag gcggtggctc agaagtatgc tatcgttttg cccccgcagc
     8281 cgacagtata ttgcacatgg tatcatgcgg gagcatcgaa tgaaaaggat cttattcaga
     8341 atgctgcctt tgctaaagaa catctggccc cctttggttt ctcggttgtt cagattgatg
     8401 acaaatggca ggatggcaag tccacaaatg gtccccgaaa aaactttacc caagtccagc
     8461 ccaacgggcc ttataaatcg ggcatgaaga ccgcagcgga taatattaaa aaactcggat
     8521 tgacacccgg catctggttt atgccttttg cggggacttt tgatgatcct tattttgcag
     8581 acaaacaata tctgtttgct aagaaagacg gcaaaccgtt tgataccaaa tggggcggca
     8641 cctgttttga tctaaccaat cctaaaacac agcagtatgt ttatgacatt gttcatcgga
     8701 ttgctcatga gtggggttat cagtatttca aaatggatgg attatatact ggaacagctg
     8761 cccgtctgat gtatatcaat gataaatatg ctgatgacca gctcggtgaa tctgttctct
     8821 acgacccgtc gatgactcat atccagtctt atcgcacagg tttacagatt gtccgcaagg
     8881 cggccggcag ggacattttt ttccttggct gctgcattcc ccagaatatg cgttcctttg
     8941 ctcctgcaat ggggctggtc gatgcaatgc gcatcggccc tgacaacaag cgtacatggg
     9001 aagcgatgct tcgcggtccc cgatacggca gcaaagtcta tttcctgcac agacgggttt
     9061 ggtataatga tcctgacccg atttatgtgc aggacgatgt tcctcttcag caggctataa
     9121 ccttatgttc ctgggttact atttcggggc aattaaacgc cagcagtacg gattatacca
     9181 aactcccccc ggaacgattg gatatcctta agcgtaccat gcctgcacac aatctcacac
     9241 cccggccggc ggatattttt gagcaggaga tccctcgcat ttggctgctg agtgatgaac
     9301 gttccggggt ccgtcgcgat gttattgcat tgtataattg ggacgataaa aatactgcag
     9361 agattcacta tccgcttcgt aaaatcgggc tggatggaga aaagcagtat atcggcttcg
     9421 attactgggc caatacattc cttgaaccat tcagcggctc tgtcaaggtc tctttgccgc
     9481 aggcaggctg cagcatcttg tctgttcgcc cggtaagttc gaatccgatg ctgctgagca
     9541 cctcccgcca tattacacag ggaatagtgg atgtcaaaga agaaaaatgg gacgaatcac
     9601 tcaaaacgct gacgggtgtc agcgaggtcg tcgccaatga ttcctacgag ctgcgcattg
     9661 cagcaatgaa aggaaatagt atctggcaac tcaaatctgt cagtgtgtca gaaccagatc
     9721 ggcaggccgg aactgctata agaataatca gccagaatgg gccttttatc cgtttgttga
     9781 ttgagacaaa agaaagtcgg caagtccgct ggaatgcgga attcaattaa accggcagat
     9841 acaggagaat agcgatgaac aggatgtatc agctacgata tagagccaac tatagtgcag
     9901 gtattatatt ttttattgca tccgcttttc tgtatgcggc ttctgccctt gaagatgcgg
     9961 ccgcaacagc ggtgccggat gcgcatttag attacatacc ggggcaggcg ccggctttct
    10021 ggtcgatcgc ggcggccgaa accgtgatgg cacgctggcc ggattactcg aaggcctatt
    10081 ttaatgcatg gacctatgtg aacggttata tgctgtgcgg atttgagcgc ctgtatcgcg
    10141 acacaggcga acgccgctac ttcgattaca tccagcgcta tatcgaccta tttgtggacg
    10201 agaacggcgc tttccgcagt gttctcaaca gcaaaggcaa tctccagaaa atatctttta
    10261 ccaatcttga taatatgatg acgggcaaca cactggtgat gctctatgaa tatacccggg
    10321 atgaacgata caaaaaagca gcagacacga ttcgccgggc tctggatgat tatccccgca
    10381 acagcgatgg cggtttctgg catgcccggg gaatgcacgg ccagatgtgg attgacggca
    10441 tttttatggg ccaaatgttt ttaacacgtt atggaaaatc tgtcggcgac agccgttatt
    10501 gctgggacga agcggctagg cagattactg tgtatgccaa acgtgcccag cgtgatgata
    10561 cagggcttta cctgcatggt atttatgaac ccggtcacgg tgacagggtc tgccgctggg
    10621 ctgatcccaa tacaggcctt tcgccggagg tttggagtga gggattgggc tggtatgctt
    10681 tgattgtcgt cgaaacactg gctgatatgc ccaaagatca tccccagcgg ccggagatag
    10741 aagcaatctt ccgccggctg gctgttggtc tgaaacgcac gcaggatgtt ccgacaggtc
    10801 gatggttcca agtggtggac aaaggcgatc ggcctgacaa ttggacggat aattccggca
    10861 gtgcgatgtt tacctatgca ctccaaagag gcattgaact gggattgatt gacaaaaacg
    10921 aatttgaacc ggttgttagg aagggctatc acggcattgt agaaaatgca cgaatcaatg
    10981 ctaaaggact tgtggatata tacagtgcct gcgatggttt aggagtgcag gccagttacg
    11041 ataagtatat taattacaaa aaaatggtca atgccaaaga agcagtggcc ggcttcctct
    11101 gggcaacaga aatagtagaa cggccgcaat tggagaaaat gaaaaaatga aatggaaacg
    11161 atttaacgaa aggagtgcga ttgtgaacag gttattccaa atgctcgcat ggacggttgc
    11221 cgcaggtatt tttgcatcat ccgtatcggc caatacagat tctgccggaa ctgcccgtgc
    11281 tgattcgcga atgtctgaaa tcccgatcca gaaggggaag gccgcatgga atgtatgcga
    11341 tttcggtgct gcgggagatg gcaaggccct cgacagtccg gccatcgaca aggctatcga
    11401 ggcctgtgca caggcaggcg gaggaactgt ctatcttcca gccggcacct atctgagcgg
    11461 aagtattcat cttaagagca atgttaatct gcatctgggt gccggtgcag tcattttggg
    11521 cgcccctcaa gaaatgaatg cctatgactt tacagaacct tgggagggaa cagcttatca
    11581 ggatggcgga catacctatt tccataacag tttgatatgg ggcgaaaatc ttacgaatgt
    11641 atcaataacc ggctatggaa tgattaacgg cggggggctt gtccgttccg atggtttact
    11701 ggaccggatg tgcggttttt caacttggaa taagcccgaa gcggcgtctg caggcagaga
    11761 aatgcagcct gtccggctgg gcaacaaggc cattgcgctg aagttatgcc gaaacgtctt
    11821 gatgcgcgat attaccatat atcacggcgg ccattttgcg attttggcga ccggatgcga
    11881 tggattgacc atcgacaatg taacgattga caccaatcgc gacggcatcg atattgactg
    11941 ctgccgcaat acaactgttt ctaactgccg aatcaacgcc cccggcgatg atgcgctgtg
    12001 tccgaaaagc agttttgcct taaagaaaaa tgtcattacg gaaaatctga cgattgtcaa
    12061 ttgtcaggtt tcaggttttg aagaaggtac tctgcttgat ggcacgatga aacctagccg
    12121 agtcaagaac ggccgcatca agtttggtac ggaagccaac ggcggttttc ggaatgtaac
    12181 agtgtccaat tgcactttcc gcggctgtcg agggctggcg ctggaggaag ttgatggcgg
    12241 cattatggaa aatattgtca tcaataacat tgcaatgatg gatgttgctg catatccgat
    12301 ttatattaca accggaaaac gcaaccgcgg accgaatgta accgagccga gccgcatgcg
    12361 gaatattctg atttcaaatg taatagcaac aggcattgag cccacgagcg gcattcagat
    12421 tacgggactt ccggaacagc ccatagaagg ggtccggttg gataatattt ggcttgtctt
    12481 caatggcggc ggcacgaaag aagatgcggc acggattccc ccggaactgg gaactggtta
    12541 tccggaaccg cgcaatatcg gggtaatgcc tgcttacggt gtatttgcac ggcatgttcg
    12601 ggacttggaa ctgcagaata taaaaataag ctttctgaag gaagatctcc ggccggcaat
    12661 ggtttgtgta gacgttgatg gccttgaaat cgacagcttt aaagcgcagc ttgctgcaga
    12721 agtccctgcg gctcggtttg aggatgttcg ccggctgatt attcgcaatt ctcctgtgct
    12781 ggataaagtg agcgtaaaat aatttcctgt actcttttca tcttaaaatg tggaaatatt
    12841 ggaaggataa acattatgaa ggttcattgc cggactttga cagattcttt cagaccgggg
    12901 aattgtttta tcacgctccg gatgtatcat tctgttgggc agacgacaat gcagatgtgc
    12961 accaagcctg tgaggggaca aatatgcggg atagctctga gtacggtata aatcgcaggc
    13021 accctgcagg tgggttatac ggtcggatgc tgattctgtg gtatacaacg atgctgtttc
    13081 gataatgtta tgcaaagaac attaaactat gttaaagaaa ggattgctga tatgttatta
    13141 aacacgaagg tttttgtaaa aacactgctg tctgtgggca ttttgtctgt ctggacggcg
    13201 tcggtgtatg gaacggatgc tgctttgcct gcaccaaagg cccaaccaga gatccgcact
    13261 cccaaagctc ccgctgagcc gcgtataaac ggaccgagca tcttcggagt caggcccggg
    13321 catccgttcc tctatcggat cccggctact ggaaactacc ccatggagtt tgccgcagat
    13381 aaactgccgg aaggtttgac gattgatggc aaaaccggac aaattacggg tacgctggct
    13441 gctcccggcc aatacgaggt aatcttgaga gcaaaaaaca gcttaggtgt cagcgaaaag
    13501 aaatttaaga ttgttgcagg cgaaactatt gctctgactc cgccgttggg ctggaacagc
    13561 tggaattgct ggggcccgac agtcgatgcc gagaaagtgc tggccgcagc gaaagccatg
    13621 gttgaatctg atctgattaa tcatggctgg acctatatca atattgatga tgcttggcag
    13681 ggtaaaaggg gaggtccatt caatgccata cagggcaatg agaagtttcc tgatatgaaa
    13741 agtttgtgtg atgctattca cgcgatggga ttaaaggtcg gcatctattc aacaccgtgg
    13801 acaacctcct atgcggagta tatcggggga tcctcagaaa atcctgatgg ttcgtgggaa
    13861 aagcccacaa tacccaaaag cggcaatgtg aacaaaaagc gactgccatg ggccatcggc
    13921 aagtattcat ttgccgtcaa cgatgccaag caatgggctg cgtggggtat cgattatctc
    13981 aaatatgact ggaatccaaa tgaagagccg gaaacacgcg aaatgtacga tgcactgcgt
    14041 tccagcggac gcgacattat cttaagtctg tccaacagca tgccttttga aaatgttccg
    14101 gtattgtcca aaattgccaa ttgctggcgt accaccggag atatccgcga taactggtgg
    14161 agcgtaacag gcatcggatt cacccagact agatgggctc cttatcagag tcaggggcac
    14221 tggaacgatc cggatatgct ggttgtcggc tatgttggcg gctggggcgg acggcaacca
    14281 aaacccagcg ggctgagccc cgatgaacaa tatacccata ttactttatg gtcgctgctg
    14341 gcttcgccgc tgctgattgg atgcgatatg acgcgtttgg atgagtttac gctgaatctg
    14401 ctgacaaacg atgaggtgct ggcggttaat caggatgcct tgggcaagca ggctgtacag
    14461 attgtagaaa caggccagcc ggttaccatt gtacgcagcg accggccgga gcagaaacgt
    14521 accctcaaac catatcaaat ttgggcacgg cctcttgaag acggatctat tgctgtcgga
    14581 ctttttaatc tcagcgacca accggcaccg attacggctt cctggaaaga cctgaaactg
    14641 actggcagac aaacggtacg cgatttgtgg cgtcaaaaag agcttgggca atatgacgaa
    14701 caattccaga tggttgttgc accacatggg gctgaactgg ttaaaattaa atctgtggtt
    14761 ttgccctagg caggggatgc tttatgtatg ttgaaaatgt atgaacagga gcacaatatg
    14821 aagtgcagat ttgtacagga tattgccgga ttagtcctga ttttgatgac tgccggtgtc
    14881 ggacatgccg aacaccgggc atcttggatg cagaaagcca aatggggtgt gatgaaccat
    14941 tatttggccg actggattgc aaagactgac aatattccta tgagtgttga gcggtggaac
    15001 cagcttgtgg ataactttga tgtagaagga ttggccgaac aggtcaaatc tgtaggagcc
    15061 gggtattata ttatcactat tggccagaac tccggatatt atctggcccc taatccggtt
    15121 tatgatcagc tcacagggat tcagccgagc aaatgttccc gccgcgattt ggtttcagat
    15181 ttatctgctg cactgacccg acgcggcatc cgactgatag tctatctgcc ggcaggtgca
    15241 ccaagccgcg accgccttgc ctgccagact ctgcaatgga aagacggagc ggatcggaac
    15301 cttgaattcc agctgcgatg ggaaaaaatt atccgtgact ggtcaatccg ctggggggat
    15361 aaggtttccg gctggtggtt tgacggctgt tattggccga acacaatgta ccgttatgaa
    15421 cagcccccga attttgcttc ttttgccgca gccgcccggg caggcaattc cgcttcaata
    15481 gtcgcattta atccgggtgt tgtggatcgt attatatctg taacacctca tgaagactat
    15541 tctgccggcg agataaatga gcccgagcgt cttatgatac gacgttctgt cgatggactg
    15601 atggacggct cacaggtcca tgtattgagc tacctcggac aaacctgggg gatgggacag
    15661 ccgcgtttta gtgctgaaca ggtatccgaa tggagccgcc gtatcgttca atatcaaggc
    15721 gcagtaacat gggatgtccc catacagccc agcggtctga tggcacaggt ttttatggac
    15781 cagttaaaag ttattggagc agcagtggca gcagtaaatc cagaaataag cagataaaat
    15841 caggattgaa aagaagaata gagctgtgtc ctatcaacag gcaatgtttg ttttggacaa
    15901 tgttttcccg cggcaaatta tttggatata gatgaatgac agctgccgaa ttcagaatat
    15961 taagttgtcc agaagcacaa gtcagctttt gctgagtaga atagttggaa cttcgtattt
    16021 tgaatactgt aaaatctatt agggtgtgta gataatgaat attttacgac tgttaatgat
    16081 tctatccgaa atcctgaatt gctatggacg cagcatcaaa actgccggga atatgaggtc
    16141 ataagatttt ttattcatgc aataaagata atagcagaaa caatcaagaa atgaggagat
    16201 cagcacaatg caggcaataa acagaaaata tgcttttgca gcgataatat tgtatgcagc
    16261 tgccttaact tttgcgcagg cagaacgtca acagcccaga caacagcgaa ccaatcgttc
    16321 aaccacacgc cgtacgcagc gggttatacc catgaaggaa gtccggctgg ttagtcccga
    16381 tggcaaggtt tccttcatta tagattccaa tcccgaacgg ctgacatggt cggcgatgat
    16441 agaaagcaca atagttattg agccgtctcc tctggatatg agaatagacg gctatgatat
    16501 ttcatccggc gttattttca atgctctgca agagtattct atagatgaag cttatccatg
    16561 gcatggtgca cacagcagag cggttaaccg atgcaatgga gccagaataa catataccca
    16621 tgatttaagc caaataccgt atattctgga ggttcgtgtt tttaatgatg gggtggcata
    16681 ccgtcatatt attgatggaa acgccgaagc tgttcgtact ccgaatgaat attcgcgttt
    16741 tattctgccg gccggaacaa cagtctggta tcatgatttg gatggtcatt atgaagccga
    16801 atacaccaaa caggacattt ccgatgtaaa agccggtcag tgggcaggac ctccgctgac
    16861 attccagctg cctgacgggg ccggctacgg ttccattact gaagccaatc tggtcaacta
    16921 ttccggtatg gcattggaaa acgatggacg acgcggctgg attatagggc ttggacatcg
    16981 tcaacctgtc aattatccct atgaactccg gtatgggcgc gatgaagcaa aacgtcttgc
    17041 tgtaccggca gcaatttccg gctcgattac gacgccatgg cgggttgttt tcattgcccg
    17101 cgacttaaat gttttggtca atagcgatat tctcccgaac ctctgtcctc cggctgatcc
    17161 gcaatatttt ccgcagggta tggatactcc ttgggtagag cccggattgt gtgtgtggga
    17221 ttatgtagat cggaactatg tacgaaatga agatgccgac cagtttcagc ggatgaaaga
    17281 cttttcccgt atggcggggc aaattggagc caaataccat attctcgaag gattcgccta
    17341 cggatggtca gatgagcaaa tacgtgaatt tacagattat tcaaaacagc agggcgttcg
    17401 ggttttattc tggcgtcaca gccggaatct ccgtacacct gagcagcgag aagaattctt
    17461 tgctcgcttg aaccgtctcg gagttgccgg cgccaagatt gatttctttg accatgaagc
    17521 caaggaaaat attgaccttt acgaggcgtt gctggaaaaa gcggctcact atcagtgtgt
    17581 tattgatttt catggcgcca ataaacccac cggccgcctt cgtacatggc ctaacgcgat
    17641 gatttatgag gctgttcggg gaatggaatc aagcagtctt cagcagcgtg cccgtcatga
    17701 gacgatttta ccctttaccc gttacttagc aggcccgtgc gattacacta caatggtctt
    17761 caatgaccgg cgcagggatt caacatgggc acatcagatt gcctgtctgg cgaccttcca
    17821 cagcccgatt cttacaatag ctgcccatcc ccaaagtgtg ctcgataatc cggccttaga
    17881 agtcattaaa agcatcaagc cggtgtggga tgagacgata gtcctgccgg actccagaat
    17941 tggtgaactg tccatatttg cacggcgcag cggggatatg tggatgctgg cgatcacggc
    18001 agctggtccg agccgaacct tgcaggtgcc gttgtcattt ttatcggagg gtgcttacaa
    18061 ggccatttat gtaagagata atatgaataa gccggatgcg gtggttgttg aaaaatcaac
    18121 agcatcaaaa aatgagatac tgactattca gctggctgac ggaggcggat ttatcggaca
    18181 atttgtgaaa taaaaaagaa ctatttgaca acatgcatcc cgatggccgt aatatcatcg
    18241 atttgattcg gcgggaaatc atggtggatt gccatgcgat taaatgcctg aagcatcggc
    18301 acgattggaa gattacaaat atccacgaat gcctgcttga attgaagctg cccttcatcg
    18361 gccgcatgag cgataagagg ttcgccgccg tctgagtata taaacagttt atcacccggg
    18421 tgcagctgga gggttttctg ctcaaattgc gcttcctcga ataccccgag aagaccgccg
    18481 cgggattcga gctggtgaat ttgaccgccg cgaatgagaa gagggtaggg gtgacctgct
    18541 cgggcgtatt caagtgtaag tgttcggatg ttgataagac cataaaagca ggttgcgaaa
    18601 aggcagccgc tcaattcctg ctgcgtcatc cgcagattca gggcggtaat gacttcctgc
    18661 ggcttaaata tggtataatc gttgccgaag gtctgacgca ttattatgga atgcttgaga
    18721 aacattgtca gcagggccgc aggcatcgaa tgtcctaccg catcggctaa ataaaaacca
    18781 atatgatctt catccagtcg ggctacatcg taaatgtcgc cggagaccca ttcggccgga
    18841 cgaaatacag ccgcccaatg aatggtatca ttatcaggca gctgggccgg cagaaaattg
    18901 cgctgaacac gtcctgccat ttcaagctgc tgagcggtgt cttttgcaag cagatagttt
    18961 tctttttgcc cttttagctt ctgcagccgt ttagaaaatt gcacactgct ttcgatgcgt
    19021 gcccaaagtt cttcgtatcc gagagactct gccctgacag ccagttgaag atggtcggtc
    19081 ttgatgcagg aaggacaacg atatagaata actggtgtgt cctgctgatc cagtttttca
    19141 aaaagacttt ggtaagcgga gacgctgtca gaatctatca tttctgcgtc aacaattact
    19201 gggccgcaaa ttgtcccttg ctgcgcataa gtataaagtt catccaatct aatacggttc
    19261 cagtgatatc cgcggctgcc cagaagaaac agcaaatcgt cagaaatcgc tcgttcgcct
    19321 gcaaacagga catttacagg ggctgccgaa ttagttgaac aggttgttaa ttgggtttcc
    19381 gtaagcattg tataattcgg aatacaaacc gctgatgata aatgtcggta attttagggg
    19441 gtgacttaat ctaactgaaa aggatttgga taattttagc ggctaattcc aagatcgcaa
    19501 ataaccggat atataacagg taatttgtct tctatcagaa aattaactga aaatggctga
    19561 tccgatacgg agcattgtgg ctccttcttc gatggcaatg gggtaatcct gactcattcc
    19621 catgctgagg tgcttaaatt tggctcctcc aatatgttcg gcccgaattt cctcgaaaag
    19681 ctctcgagct cgggcaaaac agctccttac aatatcttta tccattgtca gaggggccat
    19741 ggtcataagc ccaatcagac gaagattcgg cattgtgtca atctgttcgg ctaaatgaac
    19801 ggctgccccg acaggcactc catacttttg cggctctcca gagcagttaa cctgcattaa
    19861 cacatttata tgaattccca ttttctgggc ttggatatta atttcttcag ccaagcgcaa
    19921 ggtgtcgacg gaatggatga tcctgcagat ctgcagagtc tggcggactt tgttacgctg
    19981 caaatgccca atcatatgcc aattcacaca gcgaggaagt ccctcggctt ccgctttttc
    20041 ttttaaaaat gcatctactt cgtcggaaat ttgctttaaa tgctgtaccc tgttttcacc
    20101 cagttccaca gccccagcac gaattacttc gtgaatcgcc tcgatagagg ctgttttggt
    20161 tacgacgatg gtctggatgg ttttttcact gcgccggcag cgtgcacaaa cagaggcaat
    20221 ctcatcctgc agttttttca atttgtctgc tatcacaccc atccgcattt atccgttgac
    20281 aaacttttag ggaagaaact tattcggatt tttcagctta tccggcagat aatgatccac
    20341 aacaaagtcc agcccatgtt tggcaaaggc ctgctgctcg acacggacac ccattttcag
    20401 catctgattg atggcttcct gccatggctt atgatgtgct acgaagggat cgtttttcag
    20461 agcatctttg gctcgtttga cgtccacatc ttttaacgga tgcgtgggca gcttgtaatc
    20521 gataatatcc tgcggcgtta ctccaagaaa tcgtgcctgc ggaacacaga aaaattcatt
    20581 caaatgagca gaattacccg agccgacctt cagtgtccgg tagatattga agtatccgta
    20641 tgggtcccca tcgacgaagg catagacggg tattttcagc gtatcgctca gccgccgtat
    20701 aaaccgccgg caggcccgtg tcggtacacc gcccatgctt atcaagatgc agtcggcttt
    20761 tttccaatag tcgcatttga ccagacgctg gaaagctcca gcagtttcga tggccaaaat
    20821 gaatttagct ttgctctgga atcccagttc ttcgacattg ataggaatgg aataggcccc
    20881 tgaaccgaag cgggtacagt cgattttcag cggcttgccg ccggcatctt tgtccagaac
    20941 aatcagctga cctgccacat caccgccttt ttcttcgggc acgaagcgaa gctgctcgcg
    21001 atttacgccg aacattgctt ccatatcatc catcaccgta tcagattccg gctgttcgtc
    21061 aaagccggcc tttccccagt ttttcgagat ataataggcc tctcgtttgg ttgccatatt
    21121 gtcattatct attaattcct tagacaaggc catcattttc aaagtttggg caaaggtttt
    21181 aacggtattg actgtcagcg tacggttttt ggtatttcct tttatttcaa aatgcccttt
    21241 tttaacatga tattttacat tggacaagga gcggatggga gtgctcatcg tgggtttacg
    21301 gttggcaaga atcgagttat atacctgacg tgcattatcc gttataagct ccgcggcggt
    21361 atttttttta ttttttccca taaattaagg acactccttt acctaaatcg tgcgtattta
    21421 ttatacactg tattcattat tttttccgtt acaaacaagt tataaatcct acgaaaaaaa
    21481 tatcttggtt gcaagcctta acgtgtgctg tattgaaaac tcattacagg taatattggg
    21541 tacacaatac ctattaaagt tttttatatg ctcttaaatt cgttttttta taaaaacaat
    21601 cgttgacaaa atccgatttt atggatatac tgatttcaaa ttgataatga agtcagggga
    21661 aaagacttag ttataatcct cggcgaggcg aagtggccat ccgttttccc ccccagacgg
    21721 agccgctatc ctcgccatct ttttgcgcgg cactgaaaaa gttgggcata ggaaaaaagg
    21781 caatgtcctg tttgaatatc tgtgctaacc gcaaatggaa aggtaaacgc ttcgctggcg
    21841 aggaccgtcg aactcgcaga aaaacagcgc ctgccaagtt cccaatttca gccgtccatt
    21901 ctgtattaaa actgttttac tgacaccgac cagtgaggat ttgacatggg catcgctgtt
    21961 gccttcgctg tgttggtatc cttggcgaaa atgcggcacc agttcctcca gcgtcatcag
    22021 gatatcgtga acaacatctg gatcggcgtt ttcattgatg gtaacggcag ctgttgtgtg
    22081 ggggcagaaa acggtcagga taccggtgtc tatgccgctt cgggtgatgg ctgcttccac
    22141 ttctgacgtg atatcaatca tttcatttcg cttatgtgtt ttaacaaaga agctgtcttg
    22201 aacaactttc attggcaacc tcctacatat gctctacaac ttcaatgccc agcaattcgc
    22261 tcatgccatg ccggattgta cgggctgtca agtcacacag cagcagccgc aagggacgct
    22321 gctgtgcatc agcctcaaga acgggacatg ctgcataaaa acggctaaag gcctgtgaca
    22381 ggtcatacag atatccggtc agataattcg gccggcactc ctgtgccgcc gcatcgattg
    22441 cctgatcata gcgtatcagc agcttggcca gctctttttc cgcttcggat gtgaagctca
    22501 aagagcaaag gccggaaagc tctcgtttaa tgtcaacctc gcgccccgag gccttgcgct
    22561 caatggattt aatccgtgca taagcatact gcatatacgg agcggtattg ccgtccattg
    22621 ccagcatctt gtcaaagctg aaaacataat cgctgttgcg gttattggaa taatcggcat
    22681 acttaacagc ccctatgccg acggcttgtg caatatgttc tttctgctcg gctgaaagag
    22741 aaggattttt ttcttctaca atttttcgag cacgttcgac tgcttcgtcc agcaggtctt
    22801 taagcttgac ggttcccccg gatcgggtct tgaacggctt gccgtcctcg cccagcatgg
    22861 tgccgaatgt gacatggacc agttcagttc cgctgtcagt ccagccggcc attttggccg
    22921 ccgcaaacag catctgaaaa tgcagcgact ggcgcgcatc tgttacatag ataattgtat
    22981 ccgcatgcag ctcctgtatc cgataccgca gtgccgcaag gtctgtggtc gcatataaaa
    23041 aagccccatc gcttttctga ataataaaag gcaggggctg gccttccttg tttttgaatc
    23101 cttcagggaa tatgcatatg gctccatcgg attctgacgc caatctgcgt cccttgaatt
    23161 cttctactac cgaggccagt ttatccgcat aaaagctttc cccgcgttcg tcatcggcgg
    23221 tcaacatgat attcaagctt ttgtaaatcg gcaggtaatg ctttctggat tcctgcacaa
    23281 tctgcttcca aaccagaatc gtatcggcat cgtgattgtg aagccggaca acttcctgac
    23341 gggctttctg ttcaaatgtt ttatcttcct cggatttgcg tttggcatcc tgataaaaag
    23401 tctccagatc cgcgatatgt gcagacgaca gattatcgag gatatgcgga tactgctctt
    23461 tcagaagggc aatcagcatc ccgaattgtg tgccccaatc gccgatatga ttttggcgga
    23521 ttactgtatg cccttgaaat tcataaagac gggcgataca gtcgccgata atagtgctgc
    23581 gtaggtggcc gacatgcatc tgtttggcaa tgttgggccc ggaatagtca accacaactg
    23641 ttttggtttt ttttgttttt tcaataccca gacgctgttt gtttgaattg atttcaatca
    23701 ggcgagcagc caaaaactca tccttgagac gcagattgat aaatcccggc cctgcaatct
    23761 cgggtggttg gcagatatca gcaatatcaa gtacagctgc aacctgttga gctaattggc
    23821 ggggattggt tttgatttgt ttagcggccg gcattatgcc attggcctga taatcaccgt
    23881 atttcggctc ggcggcaggt cgaaccatcg ccgggaatga gcggcctgta acctgccgaa
    23941 tggcaccaga aatgcgttct tcaagaatat ctgtcagacg tttcattatt cctgcttttt
    24001 gtcaattttt aactgtttgg cgtcacccca gagcatctca agattgtaat aattccgatg
    24061 ttcagcatca aaaatatgga ctaccaccgt tacaaaatcc agcagaatcc actggcctcg
    24121 ttcatagccg gcgatgccga atcgctgatg tccctgtttt ttggcttcct gtgaaatttc
    24181 gtctgctacc gttcggctct ggcggtcgct ggttcccgtt gcaataacaa aatagttggc
    24241 tgcgggactg atgccccgca ggtcaagaat ggtgatatca gtgcaattgc ggtcttgggc
    24301 aatacgagcc gccgaaaggg cgagctgttt atctgttgac cgttttggtc tttttatatt
    24361 tattttggcg gtttgttttt ttactgtttt tttagatgtc ttttttgcca tagttttatt
    24421 ttctgtagac aaaataggag cgtcctcatt atgggggacg ctcctgtaca ctaatcgaaa
    24481 atacgcaatt cgtcaagcat tcggaattaa ctgctcaaac cgaacagatt gaagaaccac
    24541 tgcccgatca taggtgcgaa cttgaccact acgccgctga ataccacgct gaccattgtc
    24601 atcagcttaa tcaagatgtt caagctgggt ccagaagtat ctttgaaggg gtcgccgaca
    24661 gtatcgccta caacggctgc gccatggacc ggatttttgg ttccatcggg cagttttttg
    24721 ccgccgtatg cgcctttttc gatatacttc ttggcgttat cccatgcacc cccggcatta
    24781 ttcagtgtga ttgccagaac aaatccggct gtcaagccgc cggccagcaa gcccatcacg
    24841 cctgccacgc ctaaaagaag ccctgttgct accggaacca cgatagccaa agctgagggc
    24901 acgaccattt ccttctgtgc accggctgtg gatatagcca cgcagcgggc gtaatctggc
    24961 ttgcctttgc cttccataat gcccggaatc tcgcggaact gacgacgcac ttcattgacc
    25021 attgccccgg ctgctcggcc gaccgccttc attgtcattg cacagaagat aaaggccatc
    25081 atagccccga taaacaggcc gcaaatcagc ttgggattca taatcgtcaa atcataaacc
    25141 tcaacaaact gagaaatact ggcaactttt acctgtgccg atgtaaagcc catatagatg
    25201 ccttcgggac tctcattggc aagccgacca atccagatgc ggacctcttc aatgtaagca
    25261 gccagcagag ccatggctgt cagagcagcc gagccaatcg caaagccctt gccggtcgca
    25321 gcagtggtgt tgcccagtgc atccagagca tcggtccgtt tgcggacttc ttcgccaaga
    25381 ccgctcattt cggcattgcc gccggcgttg tcagcaatcg gtccataggc atccgtggcc
    25441 agcgtaatgc ccagcgtaga cagcatacct acagccgcaa agccgattcc gtacaggccc
    25501 atcgatacat cgctgaagcc gccggcaaag gcataggcgc aaataatgcc gatgacgata
    25561 gtgataaccg agccgccggt cgagaacatg ccgacagcta gcccatcgat gatggttgtt
    25621 gccggtccca tcacggcctg tccggcaatg cctttagtgg gattgtattc atctgacgta
    25681 tagtactctg taatctggcc gataataacc cccgcggcaa gccccgagat aaccgaccag
    25741 aaaatgcccc atgtaatcat tcccatcttg gccataatag ccagtgcaat cacaatcagt
    25801 atggagctgc ttagcgtacc ctttaaaaga gctgtcagca ggtttttctg ggatgcacct
    25861 tctttgcatt tgaccataaa gatgcccgca atggacagca gaatgccgat accggctaca
    25921 atcatcggcg ataaaacagc cattatcggg tcaattcctt tggcggccaa agaagcagcc
    25981 ggaagagctg cccccagtgc tgcagttgac agaattgagc cgcagtagct ttcatacagg
    26041 tcagcgccca tgcccgctac gtcgccgaca ttatcgccca cattatctgc aatagtcgcc
    26101 ggattgcgag ggtcatcctc gggaatgcct gcctcaacct tgccgaccaa atcggccccc
    26161 acatcagccg ctttggtgta aataccgcct ccgacacgcg caaacagcgc ttgtgttgaa
    26221 gcacccatac cgaaggtaat catggttgtt gtgatttcta ccagcttatg agcactgtca
    26281 atgccgggat gaaccagtac aaagctgccg attttgaaac ctttggttaa tccatctgga
    26341 cccagagcaa aaaagacttt atccagcaga atataccaga gagaaatatc cagcaggcca
    26401 aaacccacta ccaccagtcc catcacagca ccagagcgga atgcaacctg caatcccctg
    26461 ttcaagcttt cacttgcgcc ttgtgctgtg cggctggagg catttgttgc tgttttcatg
    26521 cccagaaaac cgcacaggcc gctaaaaaag ccccctgtta ggaaagctac cgggacaaat
    26581 ggattctgca cgcccttata ggcaagaaag gcaaaaaccg ctacaagaat aatgaaaacc
    26641 actgtaacga ccttgtattg gcggaacaaa tacgcataag ccccttcgcg aacatgatcg
    26701 gctatttcca tcatcttagg gctgcccgcc ggcgcttgca tgactttttt gtagaaatag
    26761 attgccatca gcagagagat tacagcgctg cccattgcca aataaggcgc catcggagca
    26821 aacttggcag aaactgttgc tgtctgggcg gcctgttcct gtgccatggc tgttgatgaa
    26881 agtaccatca tcggtaatcc caacaatcca atcctttcta atcctttcac gtgttgatcc
    26941 ctttcatttg aatatataag tgttaaattt aaaattgaaa acaatatagc gacttttcag
    27001 aagttttcaa cctttttgtt tatagttttt gcctgcatcg gattttgggc cgctttattc
    27061 attttgatcc gcaaaatcgg cctgtcaagt atgataatca gcattaatgg ttacataatc
    27121 tctgcttaga tcacagccgt aacagaaatc gttatgtcgg ccagacccta aatagaccgt
    27181 aattgtatgt tcttttcgac tgattatctt cgatactttt ttggagtcaa aattggtcgg
    27241 ttttccattt ctgaatacgt aaatatctcc aattttgcag gagaggcggt tgggattgag
    27301 tttgacgccg caggagccga cagcgcagat aatacggccc caattaggat cccctccatg
    27361 aatagcgcat ttaaccaagt cgtaatctgc cactgcgcgg gcagctttaa aggcatcttc
    27421 ctttgtcgca gcttccttga tttcaacctt aaacatccgt gtggcacctt ctgcatccaa
    27481 agccatctgt ttggccaaat cactgcatag gtctgccaaa gccgcggaaa aacggcgata
    27541 gccgatatct tgtttcttga ttgtcttgtt ttctgcaagg cctgaagcca aaatcattgc
    27601 cgtatcgttg gtgctttgat ggccatctac agttaagcgg ttcagggaaa gcccgacagc
    27661 atttctcagt gctttatcga gaaggatttt agtaatggct gcatctgtag tgatgacgct
    27721 gatagtcgta gccatattgg ggccaatcat tccggctccc tttgcggtgc cggctatggc
    27781 gatggtttta cctcctattt tcacagtacg catcgcctgt ttgcaccgcg tatcggttgt
    27841 cataattgct ttagcaaaag ccaatccgcc tgctgagtcg gctcgcagct ggctggcagc
    27901 ggatgcaatc ccggatgtaa ttttagataa aggcagctga tgtccgatga tgccggtgga
    27961 ggcaattagc acctgctggg gagcaatttg cagttggctg gcggttttgc ggcagatggc
    28021 ctcagccgcc gccaaaccct tggtgcctgt acaggcattg gcattgccgc tgttgataac
    28081 ggctgcatag atttttttgc tggcgatatg ctgccggcag acagcaaccg atgccgcctg
    28141 aatcgcattc gttgtaaaca tagctgctgc cgctgcacct gtagggcaat aaacaattgc
    28201 taaatctggt ttgccggact gcttaatccc gcaatggact cctgccgcca gaaatccctt
    28261 cggggctgta atactgatat ttgtcataaa agtttgtctc cactggtttt ttgttgtttg
    28321 aatcggctgt ttcacaatac gaaaagccct gtaaaaatca agttataatt ttaatcatct
    28381 tttaaaaaga taaaccgtta tgtgtacaat agaagcagct attgtttgca caaataaaaa
    28441 aggccgatgc ctttttaagg aatcggcctt ttcgaaagtc taagctgcga ttttagggcg
    28501 aagtcagcag ccaatcactt gccagaatgg caaaatcatg cagattgacg acgcagtcat
    28561 gattgatatc gccttgcagt gtatggcagg gcggtttctg gttggttgtc agctcaaagg
    28621 ctaaatccca tgaagcaccc ggagccggat attctgggat ttgaatcgga atacctttgc
    28681 cgaaaaccga accaacctgc ggcggccaga gtccctcagc tgttgcgatg atacgaaccg
    28741 catcatcatt aaacaaatgc ggccttgtct tccagcccca cggattggca atcaaagcat
    28801 tgtcatcgta aatagcggca atgctaagcc agtagatgtt cggctgttct ggattctcgc
    28861 tgggtttctg atagaaccaa tcatcttcac tgaggagctg attgaactgg aagcaggcct
    28921 cattccggaa gcagggcatt tcgggatatt cgcagcgggg gtcaatatcg tagccggcaa
    28981 agttccacac ccagttgtcg caaatgtgtt cccaaataag cttgcccgga tggctgtagg
    29041 gattattggg tccagccggt acatccgtcc atattccgat atggaatgcc ttcggcacaa
    29101 cgcgcggcaa atacggttgt gtccagccct tgaatgatcc ccaccagtgg atgtccgtaa
    29161 caggccgtcc gtccagacaa atccagtcat cggccataat gggccataga ggcggtttgt
    29221 aaattgaaac ctcatcccac cccagaatat tcggcaccgc ctgctccgga ttcagcacaa
    29281 ccggcggctg cgaatatttc ggccaagttc ctttgtaggc ctgggtgttt ttggttacca
    29341 gcagattgtg ccaataattc cagccgtaaa cgaaaatctg ctgtcccggc gggggcacag
    29401 gctgctgacc gaaaatatac ttgaaatttt catctacatc aaaaaactgc gacttggtga
    29461 tatcaaagcc tgggacattc atatagcccg tagcaacagg agaagcggca gctacacctg
    29521 tgagtgctgt attgatttta actgttgtgg gcactcccag tggaattgtg ccgggcacat
    29581 tccaccacca tgtccgcatc cggttggcga tgtcaacgat actgaaagaa acaatattga
    29641 tattgggcgt catcggcgag acggtaacct gaatggtgca gttggacaaa tctggatcaa
    29701 gcccgtaatc ccaccgccaa gcggaagcat agttgccttc ctctgccggc ggggttgtgc
    29761 cccatgccat aacaagaccg gcatcttcag gaataccccc gccgcctccg ccgccttcat
    29821 agacatacag catcccttcc ggttcaatgg gcggaataaa ctcgctgcgc ggatacggtt
    29881 caccctcaat ttcacttgtt ggattggccc agtaatccat atattgccgc cagaaagtta
    29941 gtgtagccgg tcgaatgttg gcgtctggaa gggatgaatg caatgctgtc tcccattcct
    30001 caaagctgtc aatcataaat actgccttac agggttccgg ttctccaatc aggaaatcgc
    30061 tttgatgctg ataggggtaa tatacttcgg gaccatataa ataggtttca agcggaacga
    30121 cggtaaccgt tgaatacaaa ggcatttcct ccggcggtac aagaacatcg aagaaaatgt
    30181 caaaaaatga atccggcggt tcaggccata ttttcatcgg tactggtcca aacaatgagt
    30241 gtccttttac tattgcagtt ccgatatcag gtccaggtcc ccacgtctcc acaataaacg
    30301 gcgaacccgg cgaagattcc acggcagcaa tattcatatg taccggattc cacctcaatg
    30361 caacctgaaa ttgctgtacc aaaacgggat gtacgggctg atccagataa gcgacggttg
    30421 taaccggcaa ttggcggccg gccgaaataa aaggccgggg tttaacaaca gtagacaaat
    30481 ccggattgat ttgaacattg caaaaaatcc atacatcttt tccttcggag tagctcttgc
    30541 atatccaagt ggtgctgccg gagcaaaatg ctgtggtggc cttcagctcc atcgtggctc
    30601 cgatgtaatc cagcgaatcc gagcttacaa caaagctgaa tgaaccgtct ttttctgttg
    30661 ttgtactgcc tgaagccaca ccaccgacgg cccagtttac cgtaagtgtt acagtcgctc
    30721 ccttgactga atcggtccct ccggaataaa aaacctttcc gttgacgctg taattgtatc
    30781 ctgccgacaa cgatgcacag atcagtaaaa acaatagaat tttaagaact ttcttcattt
    30841 tactaacctc ctaaatgatg ctttatgcac aagcttggca gaacgtctgc gcagcctaat
    30901 ttcttctgaa aaagacggta gattaaaaaa tggaaatagg gacttatagt ccttctgggg
    30961 tgtcctcctc ttttgacgct gacagccatc ggttttgtcc tgctgcctta tttagtagta
    31021 cattccactt gtggtaagca ctatcagccg tcttttccgg atgcaaaaac aggcagcaat
    31081 gtttttatac attataccaa cattacattc acaaataaaa tcagtaaaaa tacctaaatt
    31141 ccattaaata aaaaagtaac aagaaaagtt cttgtttgaa aacgctgcag attttatcct
    31201 tctacatgaa ggcaaatgtt gagaaatatt ttgttcaaat aaacttatct tggagaaaga
    31261 caatgaaagt atttagatgt aataggccag aattgtccat aataattttt tttatttttt
    31321 ccggatatat ccaaagtcag tctttatccg agctgccgca gtttagtcag aaaacggctt
    31381 ttgatggtca gatagagggt tttgctccag caccggccga accgctgagc ttatggtatc
    31441 gccggccggc tgaaaaatgg accgaagcgc tggcgattgg gaacgggcgg ctgggggcaa
    31501 tggttttcgg cgggattgta aatgagcggc ttcagcttaa tgaagatacg ctgtgggcgg
    31561 gggggccgta tgagccgatc aatcccgaag cgctggcggc tcttccggaa gccagacggc
    31621 tgattttcga aggacaatat cgtcaggcat cgcgattcat tgacagtaaa atgatgggca
    31681 aaccgttaac acagatgcct tatcagaccg tcggcgatgt attgctacag tttgacagtt
    31741 tcgaacgtgt tgcagattac cggcgggacc tgaatctgga tacggctgta gcaacaacat
    31801 catttactgc cgatggcgtg cgatatacgc gtcaggtatt ctccagttcg gttgatcagg
    31861 ttattgctgt gcatcttact gcagataaac cggggcatat tagctgcagg attggaatga
    31921 aaagccctca aaaggcctct gtcaaatgcg atggatctga tacactggtt ctaagcggtg
    31981 tcaacggtgg ctctgccggc attgccgggg ccttgaaatt tcaagcccgg ctgctggcaa
    32041 aaacaacagg gggaacgtta tctcccgagc aagactgcct taaaatcatt ggtgcagatt
    32101 cagctgtgct gctgatagtt attgccacga gttataaaaa gtataacgat gtcagctgcg
    32161 atccggaatt cttgaccaaa caagctattg aagccgcagg aaaacgctct gttcaagaca
    32221 tcctcaagga ccatattgct gcccaccaga gtctatttcg cagggtatct ctggatttgg
    32281 gttcgacaga ggccataaaa aatccaaccg accggcggat tgctgacttt gccaaaggta
    32341 atgacccgca gcttgctgcg ctgtatttcc aatttggccg ctatttgctg atttgcagtt
    32401 cgcgtcccgg tactcagccg gccaatctgc aggggatctg gaatgacagc atgactccgc
    32461 cgtggggaag caagtatacc attaatatta atacggagat gaattattgg cctgctgagc
    32521 cgaccaatct gggcgaatgt gtcgaaccgc tgattgcaat ggtgatggac ttgtcgcaaa
    32581 ccggccgtcg aatagcacag atgcattggg gggccggcgg ctgggtatgt catcataata
    32641 cggatctgtg gcgtgcggcg gctccaatcg acggggctac atggggattt tggccgaccg
    32701 gcggcgcttg gctgtgtaaa catctgtggg agcattacga atacaatggg gatcgtattt
    32761 atctggaaaa aatatggcct gttctaaagg aggcagctca gttctttctg gatacgctgg
    32821 ttgaagagtc aacccacaaa tggctggtga cgtgcccttc gttgtcacct gaaaatggtc
    32881 atcaatttgg agccagcata tgtgcaggcc caacaatgga ttctcagatt attcgcgatt
    32941 tatttgacag ctgtataatt gccggcggca tcctcaatac agaccagtca tttcgatcgc
    33001 agcttgttaa ggtccgcgac cggttggccc ccaatcaaat tggaaacgcc ggacaacttc
    33061 aggagtggct ggaagactgg gatatggaag cacccgataa gcatcaccgt catgtttccc
    33121 atctgtacgg cttatacccc agcgaccaga ttaatatata tgatacgccg gagctggcgg
    33181 cagcggctcg caagtctctg gaaattcgcg gcgataatgc aactggctgg ggaatcggct
    33241 ggcggctgaa tctgtgggca cgcttgttgg atgcagaaca tgcctattct attttgaagc
    33301 tccttatcgc accggagcga acctatccca atatgtttga tgcacatccg ccgtttcaga
    33361 ttgacggcaa tttcggcggc acggcgggca ttgcggaaat gatgatgcac agcagagtac
    33421 aatacagcgg cgaatctatg cttacagaga tagagctgct accagctttg ccgaagatct
    33481 ggccgaacgg ccgcatacag ggattgcgag caaaaggcgg ttttgagctt gatatcgagt
    33541 ggagagaagg ccggctgaat tatgcaaaaa ttcgctctct gctgggaaat ctctgtcggg
    33601 tccgttatgc cggccggtgt tatcccattc aaattcagaa ggggcaagaa atagtctggc
    33661 aagtgcctcc aatagcagat ggaaagtagg aataataatt ccttagattc ctgctaatga
    33721 cggggctgtc tgctcgtata attgtaaagg ctgatttttg aaatagattt atactgaatg
    33781 gcggtgttat ttgccgataa aataaaaaat ctgtaagtat tagttgtatt taagtatagg
    33841 cagactgaat taatgatatg aagggtattg aagatgagca acagagtatt cttcggtatt
    33901 ttgcttttag ctgcgaccaa cctttggggc gataatgcag ccgactggat gacttggcga
    33961 gggccgaata tgatgggtgt ttcgcccgat ggcaatccgc cgattgaatg gagcgaaaca
    34021 aaaaatattg tttggaaagt ccgtctttta ggggatgaat cgaattccac gccggttatc
    34081 gtcggcgaca ggatattttt ccagacagct gtcaagacag acaagaaagc agaatctgct
    34141 caacagcaga cggcagttca gccggcagac cagcggagag gccccggccg cggcaaaccc
    34201 acaaatattt accggtttaa tgtcgtctgt ttagaccgaa catccggcag gcaaatatgg
    34261 gaaacaactg tctgtgagat gctgccccat gaaggtcatc acaatgacca tggctttgct
    34321 tcctactcac ccataactga cggcaagctg ctctgggcca atttcggatc acgcgggatt
    34381 tattgtctgg actttgacgg caatatgaaa tggagccgtc attttggtcc aatgacgatt
    34441 cggtctggct tcggcgaagg cggttcagca gcgctggctg gagacggctt atttgtcgtc
    34501 agagaccatg aaggcgattc gtgtatttta gccctcaata aattaacagg agaaacaatt
    34561 tggcagaagc agcgcgatga aaaaacatcc tggacaaccc cgatagctgc ggaagtgggc
    34621 ggaaaacagc agattgtagt tgccggagcc aaccgcacgc gtgcatatga tgccctctcc
    34681 gggcaggtca tctgggaatg cggcggacaa acgcagaatg ttattcccac gccggttctg
    34741 ggtttcgggc ggatttactg caccagcggc tttcgcggca gtatgatgca ggccattgag
    34801 cttggacgaa cgggcgattt gaccggaacg gatgctgttg catggcaaat caccgaagcc
    34861 actccgtatg tgccgtcgcc gctgctttac ggagataagc tctatttctg ctccggcata
    34921 aatcctattc tttcatgtgt caacgcccag acaggcgctc cctattatct aaaaaaagag
    34981 cttgaacaaa tgaaaggcat ttatgcctcg cctgtcggtg cggcggggcg ggtctatttg
    35041 atcggacgaa acggtgtttg ctatgtgctg aaaaattcgg atacactgga ggtgcttgcc
    35101 gtcaatacac tggatgaccg gtttgactgt tcgccggttg tggtcggcaa ccggctgtat
    35161 ctcaaaggta agcagtactt atactgcatc gctgatgcag ccaagcagac gccgtaataa
    35221 tttacttcaa tgcttcctgc ggtagttatg tcccctgcaa gcggcagtca gcatagggag
    35281 tccatttccc aagatatacc cggtctggga caggtgcttt ttgtgccgtc taaacgggcc
    35341 aaacggatga gcatttcaat acgtccgggc tgccctgtac ggatcgctta tccgcggtgc
    35401 acgaccttaa tgcaggctgt tgcttttctg gaaaagcatc gggattgggc tcaaaagcat
    35461 gttgcccgtg taaaacagat ggaacggatg caccgccggc aggcgggaat gccggctttc
    35521 tgcggcgatg aagccggcgc cgcccaatgg ctttgcagac gtcttcaaat gctggcagac
    35581 agatatggtt tcaagttcag ccgagtttca atccgccgcc agaagacgcg atggggaagc
    35641 tgttccgcaa agaacagcat cagtcttaat attcggctgg cacatttgac gccccaactt
    35701 tgcgattatg ttttgcttca cgaactggtg cataccaaag ttaaaaatca ttcgaaacag
    35761 ttttggcgtt gccttgacgt ctgctgcgcc ggtcaggcca gacagcttga taaacagtta
    35821 aaacaatacc tttaccgctg agaagtatta ttgcagggca gcggataatg aatgcaatgc
    35881 agaacttggc aggaaaaaga cgatgaacag cttgcagctt cctcgcggtg tggtgctgga
    35941 tatggatggc gtcctgacag attccgagcc gtttatctgc cgggccgcct gtctgatgtt
    36001 tgcagagcat ggactgtctg ttgaaccgga cgattttctg ccgttcgtcg gaacgggtga
    36061 aaaccgctat cttggaggtg tggccgaaaa atataatttt cccattgata tcgaacaggc
    36121 caaaaaacgc acctatgata tctatctgga gattatcaaa ggaaatctca agccgctgcc
    36181 gggtgtacag acatttctgc gccgctgccg ctcgatgggc aagaaactgg ctatagcttc
    36241 cagcgcagac cggcggaagg tgcgcggcaa tttaattgaa atcggccttc cggaagagtg
    36301 gtttgatgcc gtggttgttg gagaagatgt ttcccgcaaa aaacccgatc caatgatttt
    36361 tcagacggct atcgagcgtt taggattgaa tccttctgaa tgtcttgtcg tggaagatgc
    36421 agttagcggc gtagaagcgg ctaagcgtgc cggcgcccgc tgtctggctc tgacgacatc
    36481 gtttccggca gagaaactcc gtgccgctga ctggattgct gctgatttgg caaatgtatg
    36541 gccccatata ttgtcttggc cggatggact atgatgcaat tggtgtcaaa cgacttacgg
    36601 gcttcaatat gtttgcgatt tgaactggcc ttgtcaacct agccggctta ccgcctgcag
    36661 gtcctgagat cccttattct ttgctccaaa tgcgcgtatt gagattcaga tcatcaacga
    36721 tgccgacagc cagcatcaaa tccctgtaat agtcctctat ccggtcgata ctgtccggcg
    36781 tgctgaaata gggcggctcg aacaggtccc gcgccagcgg aacagccgca tacatcgtcg
    36841 aaccgacaaa agacagcgaa atcttcaccc ccgccttttt ctgaaaatcg aggatacgct
    36901 gcatcaagct ggtcgagaga atataccgtg actcaatctg gtcatctcca tacacggcaa
    36961 actcccgttc aaactccggg tcttccagtt ttaccaagtc ggctcgtgca atattccatg
    37021 actgcagcat cctgccaagt cctccgaaca gtttttcagc cgtatccggc agaacaatcg
    37081 tacgtccgcg gaaatgcttg ttgaaatcgg ctgcaaaaaa aagtcccttg aaaatcgtat
    37141 gccagtgggt ttgtgtatgt cccttgctgg tgcgggttgt cgttttgtat tcggcgtgga
    37201 cttccgaaaa ggccatttga gttttgccga cgatgcctgt tacaaggtct tctccgccgt
    37261 agcggtcaat atgctggtga aacagtccgg ccgcttcaaa agtctgctga tcgatgaagc
    37321 cttcgggctg atagtcaagt cccggctcaa taaaccgcac aaggccgccg ataattttct
    37381 gcttaaatgc ctgtttgtat tttctgccgg cggcagcaaa aaaaattccg cctcctatca
    37441 gcccgccaat caaaaataga accagcacaa tcggttctgc gccctgccaa acagcaattc
    37501 ccccgataac cagtcctgtc gccgcaagga tgccggcagc cagcattgac ttctgcacaa
    37561 gctgcctgcg ctgctgctgg agctgctgca tgtccgaacg gagccggctg tcataaaacg
    37621 ctttcagttc atccgccgtc ttcatgcctt gtgtccgccc agaatggctc ctgccgaggg
    37681 gacctgccgc tggtcttggt cgatttgaaa cagcggcttt tcggtaaacc cgaacagacc
    37741 ggcaaggaga ttagtcggaa acatctgtac ggcgttgttg taatccgtaa ccgatacatt
    37801 atacgctcgc cgggctgctg aaatttgctc ttccagctca ttgagcgtac gctgcagatg
    37861 cagaaagttc tcgccggctt tcagctgggg ataattttca gcaactgccc gaacgacact
    37921 caccatccgc gtaaacgcat tttcaagctc cactttttcc tcatcattca gcaggccggc
    37981 ctgtgccttg gagcgggcat tggcaacgct ttcgaacaat gcacgctcgt ggtcggcata
    38041 tcccttgacc gttgcggcca gattcggaat cagatcccac cgtttcttca gcagggcatc
    38101 cattgtgcct aaaatattgt ggacctgatt gcgcctgccg acaagcatgt tatacatcaa
    38161 taccggtatc agcaggataa tcccgccggc gatgacaaaa ataataatgc cagacatcgc
    38221 taactcctta aataacaaaa aatcaggtaa tttcaggcca aaaaatacac catttctcca
    38281 tctgcgtcaa tgaaaatgcc ggctcggcag ccagcctatt cggtgtatta aacgccgatg
    38341 gtttccgact gtttcctgaa aatccgttca aggggcgata atacagacca taagaccgct
    38401 gtctttcagg aggctgcaaa aaataaatcc ccaaggcaga actatctgcc gttttgagcc
    38461 cgaattattt attaaatttt attgagcagg ttaaatacct ttatttttca tccgatatac
    38521 ggtaggcagg ccccgtaagg aggcataacg gcacatccgg ccggatttac cggctgggcg
    38581 acaagggata gttacaccga cgcacagagg tatgagtact gaggaaaaac cgaaattaaa
    38641 ccgcaatggc agcggctgct gccggcagaa ttgccagctg gaggcgatta ccccgctggc
    38701 aaagaaaata aactgtctgg atgtcaagcg tattgctgac atatgcattc aggagattcc
    38761 caaacttatc ggagccaaac tggcatctct ctatcttctg gatgaaaaca gcgatatcct
    38821 tcatctggag aaaaacaatc atccttttct gattaataac attgtatcgc tcaaccagaa
    38881 tccgccgtct ccgatggttg ccgccgtccg cagcaaggaa ctgctgatta tcgataatgt
    38941 cgactcccac aaaacacccc acattcacaa tacacagcgc atttttgcca aaaattacca
    39001 gacgaaaagc tgcattattg cgccactgat ctgccacggt cgggtggtcg gcgtgctcaa
    39061 tctcagcgac aaagacgaca aaggcccctt caccgaagat gatatcgccg ttgtggaact
    39121 gttccgccag ctcattggcg cctcgatcgg caatatcagc ttgttcgaaa aaacccagcg
    39181 gcaggccaaa accgatggcc tgacgggaat gctcaatcac agaatgttct atgaaatgct
    39241 tgaaatcgaa cttcgccgag cccagcggta cggcggaaag ctttcgatta ttatgatcga
    39301 tatcgacaat ctcaagccga ttaatgacaa ttacggccac cgggccggcg atatggctat
    39361 caagcagatt gcccgccgca tcagcgtctg catccgtcag attgatatcg ccgcccgcta
    39421 cggcggagat gaatttgccg taattctgcc caatacagcc ctgtccgatg cagcggtagt
    39481 ggccgagcgt atggtcgctg ttaccacagg aacaccaatg ctctgggaac agcaccgcat
    39541 ccccctgtcc atcagcgtcg gagtggggca gtatgacggc gaaaactgcg ccggtgatgt
    39601 aaccaagacg accgatcagg ccctttatgc cgccaaacag gccggcaagg gccgtgtctc
    39661 tgtcttcgag cccagcaaaa ctgccgcccg ctgattgcct cgttttttgt caaaacccgc
    39721 cttcgctaaa ctttgctatt gccttgcgtt ttcctgcctt cgccgctgaa aaaacgcctg
    39781 aagctgcttt tggcattctt cctgcaggat gccgctggtt acctccagcc ggtggttaag
    39841 ccgcgggtcc tgcacaatat tgtacagcga cttgacagcg cccgtcttag ggtcatccgt
    39901 gccgtaaacc aaccggtcca gccgtcctaa aacaagcgct ccggcacaca tcggacaagg
    39961 ctccagcgtc acgtaaatcg tgcagcccgt cagccgccag ctgctgatgt agtctgccgc
    40021 ctgcgtcagg gcgataattt ctgcatgcgc cgtcgggtcg ctcagcagct cccgctggtt
    40081 gtatccttta gcaatcaccc ggttttggta cacaatcacg catccgatcg gcacatcgcc
    40141 gttctcttcg gcgatatagg cctgatcaat ggccatccgc atatatcgct ggtcgctttg
    40201 taggtctatg cttggttctg tcattataat atcctgtatt ccattatgtt ctgctttgcg
    40261 gtaagacgtc atcaaaatat acctgcaccg cccgccgcag tacagatgaa aaacactggg
    40321 tatttatcat ttgtacctac tgtgcacaat attcagaatc agccgcatcg acaatctgta
    40381 ttgtccgata aaaatcaaga aaaacacttg aaattggaca acgtttccgt ataatgctat
    40441 cctgaattga aggaatgagg aataatgatg ccaacatctg ggattaagag ggtaaagcca
    40501 gccgcagcag gacgatacag ccgcaaagct ggggtttttt ctcgattggt ttttctgtca
    40561 ggtcaaacaa cgcttctttt actcagcaag ggggagactt tgtacagcaa gccattttca
    40621 gacaataggt taaatctgta ttgggacgcc ggttttctca ctccgacaat cccgctgcca
    40681 agcggattta gaggcttttt atggagaaaa atataggatt tgtccgataa agtcgttaat
    40741 gtcctgatgt cagggacata aaaaacatta atttttttct taggaggtgt tatatgttaa
    40801 gtaaaaaggt agtttggggt aatggatttt tatgtctggc cgcggttctg tgtctgtcgc
    40861 cttttgcagc agctctggat gtgtgcggac aagggtttgt gtttattgat ggttctgaag
    40921 atccggttgt ggacatcaat tgtgaaacgg gctacacatt tgtgtataat ggtgcgacgg
    40981 tgaatcttag agaaggtgcc catatatctg aaaatttagg tggatcagga ggtggtattc
    41041 tgacgtcagg gggcaatatt acaattaata tttatgccgg tacgatagat accatgctca
    41101 atacgacatc aacagatatt gtaaccattt acggttcatc ttttgtatta ggcggtgacc
    41161 agctttctgc tgggcaaact cagattgtgg ggccattggg tacagatggt aatccgatgc
    41221 tgttgacagg tgatggaaca gaagggccta tatcaattcc ccttgtctta gaggaaggag
    41281 caatagttaa tctgaatata tcccagacgg cgcctgatat ttatgttcag cctgcccaag
    41341 gtatttggga ttttggcaat gtcgaactcg gacaaagctc aacggttgtg gttgaaatct
    41401 ataatcacgg gaatgctgac ctgatagtat atgatgtgca gctgacaggg gatgaagcgt
    41461 ttactattga tggtgtgttt ccaacacagg ataatccatt gattattgcg cctaatacat
    41521 caaaaggcgt tgattttgaa gttaccttcg cccccgcctc gcagggctat aaatctgcca
    41581 cggttacaat cataagcaat gatgaagatg aaggaatagt agaagtgtct ctttatggga
    41641 tgggtattgc tgctgaagtg ccgcctttgg tgcagataca aaatatcttg gcttattttg
    41701 atgccagtgc ggccgacggc acactgctcg gatatggacc cggcaacagc cccgacaaac
    41761 ggctcaatgc cctgcgtaat atgatcgaag cagccgatga tttaattcag gacggagcgt
    41821 atgacttggc tattatccag cttgaggcga ttgccaagaa aacagacggc attgccaaac
    41881 cgcaggattt tgctgttggc gaagcggtgc ctgtattgaa cgcaatgatt aatgacttga
    41941 tggccgctct tgcttcgtga ggatttgatg agataaaggc agcaaaggcg aggataactt
    42001 cctcgccttt ttttataccc ccaaggggac tcgaacccct gttaccggga tgagaacccg
    42061 gcgtcctggg ccgctagacg atgggggcgg ctgagctgac attatccccc tcatgtaaat
    42121 tattttcggc taaaatagcg gcgggtggat tcgaaccacc gaccctgggc ttatgaatcc
    42181 catgctctaa ccagctgagc tacaccgccg tgtaattgcc tcagaggcga ataacctaat
    42241 caaaaaaacc aacattagca agaaaattct tcttttttaa gacggatttg atgatttttc
    42301 tcaagatgcc ggacactaat ccgattaaat ctgtcccatt tctgcagaat agattctgtc
    42361 cctgcagctt tatgaatgta agaccggctg ttggcagatg gccgcaggcg gcaggactgg
    42421 ataccttaac acaatagata aacggctgca tttccggcaa actctgtctg cttttggtag
    42481 ccaatatcgc tcgatcattt gagcattttc attttttgtt ttcttctggt tatgccgaaa
    42541 tcattctttc aggactgccc cgaggggccg gttttgccga caggctattg cttaaacagc
    42601 gtcaggtcaa cagcctctgc catcatctcg tacccgcggt cattgagatg tagatggtcg
    42661 ccgctgtcag cttccggcag cagcagttgg ggattggcgg gattatgcag ggccgcatca
    42721 aaatcaatta ccgcatcaaa ggcgccgctg gttcgaatcc agtggttgat tatctgccgc
    42781 tgggcctgca gctggggatt gtcataaaac gacctgccgc agggcagcag cgttgcccca
    42841 tacacccgca gcccgcgggc ccgcgagcgt tctataattt gctcgtagcc ggcgataatc
    42901 tgccgggcgt ctgctgtgcc tgtgcccaag tcgttgatgc cttccagcac gataatccag
    42961 cgtgccgccg gctgatcaag cacatcacga tccagccgct caagggcggt cggccctatc
    43021 ccgccggcca gcaggcagtt gccgccgatg ccctgattaa gcacaccgat gcaggcggtt
    43081 gtcgagtctg cctgcagacg ctgcgccaga taattcggcc agcggcggtt ggcaccttct
    43141 gtcgagcctt tgccgtcggt gagtgaatcg cccagacaca ccaccgcccc gcaggagctc
    43201 ggcgccagta catccagccc gcacagccag taccagcatt tgctggctgt attctgcggc
    43261 aaggaggcgg catctgccgc attgccggcc tgtatgaacg ccgcctcgcc tctggcgctg
    43321 cgatggccgg tggtcttggc aggagctttg gagatataaa gcgataccgc taaatcggaa
    43381 ttgggcttca ggtgaaatgc ggccacatcg gatgcaatga cagctccggc aggcactgca
    43441 attgacggct tgccgccgaa tgtcagcggg gtaagagtat cagccgcaat aacaccggcg
    43501 ccggcagact cggcaataaa ggcccgctca atatgcagtg tgtccgatgc atcggcaaag
    43561 ctgttggaaa agcgaacccg cagatgcctg ccgccggcag agacccgtat aacctgacgg
    43621 attgttgtgt tgtccagcgt ttgataatcc gggggcatca gttcggcctc taccggctgt
    43681 gccgaggccg cccacgttcc cagccaatgc cggctgtttt ggatgaactg tctggaacat
    43741 cccgatccgc ctgcacagat aaccaaaacg agtagaatgg tcccaatctg ttttttcata
    43801 aaatcctcac attctgcaat attaattctg ccgaattgtt cggctgcaac cattattata
    43861 aaaataaacg gcattttgaa aagaacaatc agcagcttaa aaaagaggtg gattttgcat
    43921 gccggatgtg tcatactatg cggggttgac gataacgtgc gttgggagtt cgctgttgaa
    43981 agacagaatg ttcattttgc tgctgctggg tttttgggcc ggctgtgccc cccagccgat
    44041 gaagccccag cagccgctgt gcgctggtaa atcgtcaatt caagaggccg ccgccgctct
    44101 ggctctgcag aggcagaatc tccagccctt ccgcgccagc gccgattgca ccatctcgtg
    44161 gatagaccag cagggcaaga aacgggatga gccgatacgc agcgccgcca tcgcctttgt
    44221 gcccgacagc agggtcgatt tcatcggtca gctgctgttt aaggaagccc gcttcggcgc
    44281 caatgaatcg gagttttggc ttcgcatcaa ggccgagctg gatacctatt ggtggggcac
    44341 tcgtgcacag gccgcccaat gccggcaagg gctgatagtc aatcccgatt cccttgtgga
    44401 agcgctgggt attgttgctg taggaccgga ctggaagctg tttcaccgcg atggctttga
    44461 tatcgtcagc ttgtatgaat ccggccgtat tatcaagcgc gcctatatca acgcatgcga
    44521 ttaccgcgtc gagcagattg agtattttga tgacgaacaa atccgaagag tgcaggtcca
    44581 gcttggggag tatgccgccg gtgaaacggg tattgtggca cccactcgca ttcgggcggc
    44641 atcgtttgac cgaatggggc aggaagaaag cgctgtgctg attcggctta aaaatattcg
    44701 gccgctgccg ccggagcaga tcggcagaaa attgtttgtc cgtcccggac gggacggcta
    44761 tgcgaatgtg taccggttgg atgagaactg tgagtttgca caagaggaag gacaatgacg
    44821 cgacagacgc ttacaagccg gcttggccaa ggcctctttt ttgttgatgg ggcaatgggt
    44881 acgcagctta tcgaacaggg agcccgggcc ggcggatgca atgatatgct gaatctggat
    44941 tccccggcgc tggtacgagc ggtgcatcag aggtatcttg atgccggcgt tgatgcggtt
    45001 atcaccaaca cctttggggc caatgcactg tcgctgggcc gctatgggct ggcggataag
    45061 acctatgcag tcaaccgagc cggagccgaa ctggcacggc agacagccgg agacgaccgc
    45121 tatgtcttgg gcgacatcgg cccctgcggc gattttctcg agccgctggg aacaataacc
    45181 ggccagcagc ttcttgcggc atttgcccgg caggccgaag gtctccgcga tggcggcgtt
    45241 gacggcttta ttatcgagac catgacagcc cttgaagaag tccgcacggc aatcgctgcc
    45301 gtcaggacgg tttcggatga cctgccgatt ttggtatcgc tggcctatga tgcagccgcc
    45361 gacggaaggg cacgaactat gatgggcatt acgcctgctc aagctgttga gtacttgtcc
    45421 ccgctgggaa ttgccggact gggcttcaac tgcggcacgc tggatatggc cggctatgtc
    45481 aagctggcag gtgattatgc agccgccctt aagggcacag gtctgcttct tttggccgag
    45541 ccgaatgccg gccgcccgca tttggaaggc gataaagctg tttacactct cagccccgat
    45601 gcctttgctc tggcgctggt tcaaatccgg caggccgggg cctctattct gggcggctgc
    45661 tgcggcacaa gccccgccca cttgtcggca gctgtccaaa aactgacccg ctgacagcaa
    45721 aaaagggata tcgattcccg cagagaatta tcgggaaaat tacttttcac ggcggatttt
    45781 attagcaatt gccccgacga taggatatta taaagcggtt gtagagtgaa aggtcagttg
    45841 cctttctttt cctttaacct gacctgttcc aaggtcgaga acaaaagcgg aggccattat
    45901 ggcgaaaaag ttgtatgttg gaaatttgga ttacagtatc ggtacgaaca caattacgga
    45961 atggttttcg gcctatggga ctgtagaaag cgtcaatctt gtcaaggatc gccaaaccga
    46021 ccagagcaaa ggctacggat ttgtcgaaat gagcaccgac gaagaagcca gcgcagcaat
    46081 tgaaggatta aacgataccg agcatcttgg acgcaatatc aaggtagcag cggctaatcc
    46141 tcccaagaca aaagccccgc gccgtgctgg aggtttcagc agcggtcgcc gtttcagcgg
    46201 acaaggcggt ccccgcccag gcggccgtcc cggcggccag cggcggtttg gcggcggcga
    46261 tagaggcggc agagacagcc gtcactaatt ttagtatctg atttcgcagc aaaaggcaga
    46321 ttcctgttct aaggtgcggg aatctgcttt tttgtggttt ttgagaatga ttgccgatgg
    46381 attgaaaaga tttaatgggc ggtgaaaagc agagggttct cttttttcaa tccggcggcc
    46441 cgcggcctgc catcttaatg aggtaaaatt tgttttgttt ttttatcatt aactgacccc
    46501 ccaaggtcat tgaccattaa tacaatgtga attataaagc aattgcaaag atttgtcaat
    46561 gaaatgtttt tgaattatta agcatcgtta gtaaaaatca caaaacacct taatatatct
    46621 tttgcagctg tgttattata agacgataat ataagacgta tcagaatgca gatgtaattt
    46681 ttttagtttt ttagatcgac tgtgttgtac tttgtagtaa tttttgatta gcccgaaaaa
    46741 taggtcagga tgtgacaagt gtgcggacct gcgaatgata agagaacgaa taacaatttg
    46801 cggcagggtt caaggtgttg gctgcaggcc ctttgtgttt cgtctggcac aagaagccgg
    46861 attatgtggt tatgtccgca atgacacaca gggtgtattc atcgaagtgc agggcagcaa
    46921 agcggctata gagcaattca aaagccagct gctggattgc caaaatcccg cttatccccc
    46981 gttgctgaaa attgtatcgc tgacagtctg ccccctggat ccggtcagcg gcgaaaacgg
    47041 tttttctatt gccgatagcg accccgccgg aacacctgcc agtcaggtga cccccgattg
    47101 tgctgtctgc aaggcctgcc tgaatgaaat gaacaatcca gccgacaggc gttaccgcta
    47161 tccgtttatt aactgcaccc attgcgggcc gcgctactcg attgttaggg cgattcccta
    47221 tgaccgggct aatacaacaa tggatgcatt ttcaatgtgc agtgtctgcc ggcggcagta
    47281 tgccgatgtg ctggatcggc gttttcacgc gcagccggtt gcctgcccag tctgcgggcc
    47341 ggccatacgt ttaaccgatg cagacggcaa tacgatagaa gctgattccg acaaggccat
    47401 cgcccgctgt gcagaaatcc tgttgcgcgg gggcattgcc gccataaagg ggctgggcgg
    47461 ctatcatctg gctgcagatg cgcataacga gagggcagtc agtgaattac gccgccgcaa
    47521 acggcgcgat gcgaagccat ttgcgctgat ggccgcttcg gttgaaactg tcagctgcta
    47581 tgcgattgtg gatcctctca gccggcagtt gctcgaaagt ccccaggcgc cgattgtgtt
    47641 actggataaa atacagccca atgcactggc accttctgtg gccaacggcc tccatcggct
    47701 cggatttatg ctgtgttata ctcctctgca ttatctcttg tttgcccagc cgggcatcgc
    47761 tgtgctggtc atgaccagcg ccaatctgtc cgatgagccg cttatctgca aggaccagca
    47821 agctctggat gaattaggcg gcattgcaga tgtcttcctg atgcacaacc gcgagattta
    47881 ccgtcccata gatgactctg tgcttcatac ggtcaataac cagccggcct ttttacggag
    47941 ggcccggggt tatgtgcctg ctcctgtctt ccgaagtaaa ccggcttcca aagacatttt
    48001 tgccgccggc ggcgatttaa aaaatactat ctgttttgtc aaaggcaatc agtatcttgt
    48061 cagcgagcat atcggagatt tggccgacgc aaaggttttt cgccattatg tccgctctat
    48121 cgaacacctc cagcagttgt tcgatgtcag accgcaggca gcagtatgcg atctgcatcc
    48181 cggctattta tcttcccagt ttgctcaaca agccggcatt cccctcctgc gtgtccagca
    48241 tcattgggcg catattgcct cggttttagc cgactgcaat gaacaggggc cggtaattgg
    48301 attggcagct gatggaaccg gttatggaac tgatggggct gtctggggct gtgaatgcct
    48361 gattgcttct ttaacagagt tcgagcgttt tggacacttg gcctattacc cgctggccgg
    48421 cggcgacaaa gcagctcaag aagctatccg cccgctggtg ggtttgcttg cccctgacgg
    48481 caatttcggc tgcctgacgg agtatatgga cattctgaac cgcatcgaac cggatgtcga
    48541 aaaaatccat cttatttgtc ttcagataca aaagcgatta aacactgtgg cgacatcaag
    48601 tctgggccgg ctgtttgatg ccgccgcagc actggcaggg gcaggagctg tcaaccgata
    48661 tgaggcccag cttccgatgc atctggaagc aatgaccgcc cggggagcag aagagggcta
    48721 tcctgttcag cttgattcgg agggtgccca gatatgttta tcacctgcac cgattataaa
    48781 agccatgata aaggacatca gagatggcat ggatgcatct gtcatcgccg cccgctttca
    48841 aaatgccgtc tgcgaaggcc ttctccagat ggctttctgt gcccgccaaa agcggcagct
    48901 gaatactgtt gcactatccg gcggggtctt ctgcaatcgg tatttggcca atcgcctgat
    48961 cgtccggttg cagcaggagc aatttcgtgt attatggaaa caatccgtgc ctgtcagcga
    49021 cggcggaatt tcgctgggac aggcggcaat cgcagcagaa atgctggaca aaggcaaagg
    49081 ttggtgatgc aatgtgttta gcagtaccgg cacgaattat agagaaaaac ggggatagag
    49141 cagtagcgga tgccatgggc aaccgctgga cgattcgcat tgccctgaca ccggatatcg
    49201 aggtgggcga tatcgtcctt attcacgccg gctatgccat cagtaaagtg gatccgcagg
    49261 aagcccgcaa gacatgggaa ctgtttgagg aaatcgcccg atacgaggca cagcaaaaca
    49321 cagcccatcc caaggagacc taccagtaat gctcgaacgg attgaaaaaa catgtcagac
    49381 cctacattcg gcctgcagcc gcctgagcag acagataaac ataatggaag tctgcggcac
    49441 gcatactgtt gccattttcc gcagcggcat tcgttccctg ctgccggata atctcaggct
    49501 gctcagcggt ccgggctgtc ctgtctgcgt tacagaccag tcctatattg acactctgct
    49561 tcagatagcg cagcgcacgg atgtcctgat tgctacatac ggcgatatga tgcgtgtgcc
    49621 gggaagcaca ggttcgatgg agtctctgcc gaatgtccat gtggttctca gcgccgatga
    49681 tgctctgatg ctggcgcaga aaaaccccga caaaatcgtt gtttttgccg cagtgggctt
    49741 tgagacaact gccccggcaa cagcggtggt ggtgcaggat gctgctcggg aaggcattga
    49801 taatttttgc attctctgcg gccacaaacg ggtgattcct gcgatgaaaa ctttggtggc
    49861 cgaccgcaac gacaaaatcg atgcctttct ctgtcccggt cacgtcagtg tgatcctggg
    49921 ctcggatgcc tatataccga ttgctcaggt attctgccgt ccttgtgtgg cagccggttt
    49981 tgaacctatg cagattttgg aaggattggc tgccatctgc cgtcagaaag tcgaaggcaa
    50041 agcgtctgtc gagtcggtct atggggctgt ggtaacaccg cagggcaata aaacagcgca
    50101 gcagttgttg gctgatgtat ttgagttcag cgacgatccg tggcggggct taggcgtcat
    50161 caaagacgga tcattcaaac tccgcaaggc tttcaagcag tttgatgcag cagaacggtt
    50221 tgccatagag caaatcccgg ccccagaagc cggcgcctgc cgctgcgggc aggtgctttg
    50281 cgggctgatt gatccgccgc agtgtccgct gtttggccgc cgctgcacgc ctgatgcacc
    50341 tgttggtccc tgtatggtca gcaccgaagg cgcctgtgcg gcttggtaca aatacggtca
    50401 atgaaatttt cctaattgta aatcctaaac acataaatat gaatgagcat aaaacaatta
    50461 tgctggccca tggcgggggc ggacggctga tggaagaact tatcagccga acaatagtgc
    50521 cgcgattggg cagcgggcag tcggtgcagc tgacggattc tgcactgctg gatgtgccgg
    50581 ccggcaagct gtgctttacc accgacagct ttgtcgtcaa gcccttgttt ttcagcggcg
    50641 gcgatatcgg caagctggct gtctgcggca cagtgaatga tttggcagta gccggtgctc
    50701 ggcctatggc gttaagtctg tcgctgatcc tcgaggaagg ccttgcaatg gataccctcg
    50761 aggcggttct tgacagcatt gcccaaacag cccgtgcagc aggagtacag attataaccg
    50821 gtgataccaa agtggtcgag aagggtgcag ccgaccaaat ctttatcaac acggccggca
    50881 tcggaatgcg gctccaaaac gccaagccgg ggtatgaaca tatccagccc ggcgatgtga
    50941 tactccttaa cggtccgctt ggcagccacg gattaacgat tctgctgcag cggcagggtc
    51001 tggagtttca aaccatgctt gccagcgatt gtgcctgcct gcacgaactg acggcgatgc
    51061 tgtatgaaaa tattggccgg tctgtgaagt ttatgcgaga tcccacccgc ggcggcgctg
    51121 cggctgtgct taacgaaatc gcccgtgccg catcggtctg tatcgaaatt aaggaaacag
    51181 atttgccggc agacaaaacg gttcaggcag cagcggatat gctgggtttt gatatattga
    51241 atgttgccaa cgaaggcaag gtgctggcag tggtcgcccc cgaagcggct gaaacggcat
    51301 tggccctgtg gcgctctcac ccgctcggtc gggatgcagc cataatcggc actattaccc
    51361 gctctgaaga caaggcgctg gtggaactga ttacacgcat tggcggcaga cgcattgtgc
    51421 cgacgccgta tggccgggaa cttccgagga tttgctgatg cacgaaacag ccattgcaga
    51481 aagcattgtc cgaacgattc tcgaaaatgc ccgccaattg caggcaaagc ccctgagcgc
    51541 ccaaatctcc tgcggccagt tcaataccgt caatgatgag ctgatgcaga cggcctttca
    51601 ggccgccgcc gcaggcactc tgtgcgaggg gatgacgctg acaatccgcc atatcccgct
    51661 gcaggcctgc tgccgacaat gcaaaaccgt atttgagttt gatgtctaca agcccgtctg
    51721 cactgcctgc gggggggata aatatgattt taaacccgat gcgccgctgc ttttggaaac
    51781 aatcgaattt gaggacaatg caggctttgc aaaggggtaa taaagcccgt aagaaggaac
    51841 ggatgaatat gaaaatatca cttaaccaga ggattttaag ccgcaatgaa gaatgtgccc
    51901 ggaaaaataa agtttttttt gatgaatatc agctgttctg tttaaatatt atatcgtccc
    51961 ccggtgccgg caagacaact cttcttaccc gcacaattac agacttaaaa ggccgtcttt
    52021 ctgtcggagt catagaaggt gatattaaaa ccgataccga tgcacgcaaa atccgcgcag
    52081 ctggagcaca ggccgttcag attgaaacgc agggcgcttg tcatttgtcc gctgagcagg
    52141 tgcacaatgc tctcacgctg ctggatgtca agactttgga tgtgattata attgagaatg
    52201 tcggcaatct ggtctgtccg tctgctttcg accttggcga gcaccggcgg gcggtggtgc
    52261 tgtcgattcc cgaaggcgat gacaagcctg tcaaatatcc cggcacattt actggagccg
    52321 atgttattct gattaacaaa attgatttag ccgactatat tccttttgac cttgagaaag
    52381 ttttggccga cattcatatg ctcaatcctc atgcagctgt atttcaggta tccgccgcaa
    52441 cagggcaggg gatgaatgca tggtacgatt ggcttctggc cgaatgccga aagcacgacg
    52501 gccgtcagag tgaatcgatc gaaaagccgt ctcggtaggt ttttttgaga aacgcattgg
    52561 cttggggcag attggtaaat tccctttttt cggcatccca cagcagtttc tgccggcaca
    52621 gcgactcccg cagttccatc cgcagggcga tattgcccag caggacaact tctgtcagca
    52681 gtccggcaaa gccgaaattg gagccggcgg gcgtcccgcc tttgcaggct gcaatccatt
    52741 cttcataatg gccaatggaa cgcggcaggg ttttaggcgg cggagtgtac tcttgcattt
    52801 tggattcagg tataatccgc ccgtccagaa tctttccctt ctggccgata taaagacacc
    52861 ccccgatgcc aagctgcctg cccggttcca gttcttccgg acgcggcggc ttcagcccgc
    52921 catcgtacca agttactgtt accggcggca tatcctcccg tgccccgaat tcataagtta
    52981 ccattgatgc taccggatac gtttctcggt tgacctgcgt gcaggatgcc tgcaccgata
    53041 gcggataccg cagtttcaaa gcccgaaaaa cgcagtccaa tgcatgacag ccgatatcgc
    53101 ccaatgcccc tgtgccgaaa tcccaccacc cgcgccagcg gaaggggtgg tagcagggat
    53161 gataaggcct atgcggggct ggtcccagcc acaagtccca atccagacct gccggaatat
    53221 ccgctgcttc agcaggccgt tcgataccct gcggccaata tgtatcaaaa agcccattca
    53281 tcgggcgatc tgtccataca tgcacctcct gaacagcccc aatagcccca tcggcaatca
    53341 tttcttgaat ccgccgcgga ccttcgtcgg cctgcccctg attgcccatc tgcgtagcaa
    53401 ctttcatctg gtcggctgtc cgactcatcc gataagcttc ttccaccgtg tgtgtcagcg
    53461 gtttttgaca atacacatgc ttgcccagcc gcattgctgt cagagaaatc agcgcatgcg
    53521 tatggtccgg cgtggcgacg acaactgcat cgatgttctt ttccttctcc agcatcacac
    53581 gataatctcg ataccgcttc gcgtgcggaa attgattgta tgtcttggcc gcatagttgt
    53641 catcgacatc gcacaaggcg acaatattct gggagctgac actttgggca tccgagtagc
    53701 ccttaccacc caccccaatg caggcaatgt tgagtttttc gcttggcggc gtctgattgg
    53761 gacctccgag aaccgaacgc ggcacaatgg taaaggcccc tatggaaaga gcactttgct
    53821 taagcattct tcggcgggat attttctttg gtttcggagg ataaaatgcc attatattgc
    53881 ttctcatttt cattttttac tgtgaataat aatattaaga tactcctcca aagagggaaa
    53941 atcaaccttc tgtaaaactt tttgctaaac aagccaaaaa atcataattt ctccagaata
    54001 cggtcttgcc agaggcggtt ttttttgtta ataatacact ttcaatcggg gccatagctc
    54061 agttgggaga gcgcttgcat ggcatgcaag aggtcgtgag ttcgaatct
//
LOCUS       DAOTBO010000009        51280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_550_length_51280_cov_5.358614, whole genome shotgun sequence.
ACCESSION   DAOTBO010000009 DAOTBO010000000
VERSION     DAOTBO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 51280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51280
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_51280_cov_5.358614"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLE88_02015"
     CDS             <1..307
                     /locus_tag="PLE88_02015"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75272.1"
                     /translation="GSAGYRGFVTDCARRWLQNHTCDPASTAIFACGPEAMLKSTARL
                     AGDFSLPCQISMERMMACGIGLCQSCAVEVKNAETRYQLCCKDGPVFDARNVIFQAD"
     gene            307..1239
                     /locus_tag="PLE88_02020"
     CDS             307..1239
                     /locus_tag="PLE88_02020"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOM75273.1"
                     /translation="MEADTTLKTEFAGLELASPVFLASGTCGYADELAEFMDLSILGG
                     ITTKSITLESRKGNPTPRIVETCGGMLNAIGLANMGLEQFQNQMLPVLEKLPTRVFVN
                     LAGKTIEEYAAVARALSAETAIDGFELNISCPNVKKGGITFGTDPVLIREITAAVKKE
                     CGKKPLMVKLTPMVTNITATATAAIEGGADALSMINTFTAMVIDIHQRRPVLANRTGG
                     LSGPAIKPIAVYLVSKVYQEAAKDAGIPILGMGGIMTASDALEFFIAGASAVAIGTAT
                     FIYPDTARRVIEGLRCYCTQKGFSRISDLVGTLQ"
     gene            1327..1635
                     /locus_tag="PLE88_02025"
     CDS             1327..1635
                     /locus_tag="PLE88_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75274.1"
                     /translation="MSQSEILKLLMDYFPTAVNTGRSLVFISEDWRIELIERKNPFFG
                     QNITNTPIIRVKVFKKALNGEFLAGHYEDFQILSINDLALQIERYIQNAVGTNIRENA
                     "
     gene            1628..1936
                     /locus_tag="PLE88_02030"
     CDS             1628..1936
                     /locus_tag="PLE88_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOM75275.1"
                     /translation="MHNLLSAICREHPQGVEITVKAVAGSSRTAIAGIHGQMLKIKVA
                     APPEKGKANKAIVELLANKLKLKKSDVLIQSGHTSCVKQVLFQGITVHDVEILFNEKT
                     "
     gene            1965..3626
                     /locus_tag="PLE88_02035"
     CDS             1965..3626
                     /locus_tag="PLE88_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75276.1"
                     /translation="MLAAASLLLSLQADGVTLANSQKQGLRAYTIEGMLKLPDNEIDI
                     GTAALILSRQWGSQRTLYVYRRKIDDMAEAILKKLHEQHLPADHRAVSVMNTYLFEEL
                     GFSSVETADNPEDLFLHTVLDKKRGYCLSLSILYLAVAERLGLPVYGVVVPGHFFVRY
                     DDGRHRMNIETTSKGAGADDAHYIATFKPPANPQMLYMKNLTPKQTLGCFFNNLGNSY
                     TAVGDTEKAFESLLRAVQINPLLSEANMNLGNIYLQKKMPHQAIAQYEKALSILGSDA
                     KVMNNLGSAYMQLSNYPRAESYYKTALSLDPQYTDVYRNLAQALQMQGKYEEAVSQLQ
                     AAVVLNPKDAAAFALLGQICRQLQRFPDAEKYLSKALSLDASLASVRISLGYLYLDQS
                     RLEQAQEEFSSALQSADTAAQAYFGLAQIYHQKGQAEDEIAAYEQALAIDPSMTAALQ
                     NLGNAYSRKGNDMAAIDAYKKAIRINPKNPDLHYNLGIALAKMQHHEQAIAAFLEAIQ
                     LDANNAAAYNGLAISYYQSGNKELARIYAQKAKSLGFEISDILLK"
     gene            3645..4496
                     /locus_tag="PLE88_02040"
     CDS             3645..4496
                     /locus_tag="PLE88_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOM75277.1"
                     /translation="MNKQQKILTGIVFILFVLIVAAGILIKIYGNEALRIGIEKASQS
                     ALQVKTRLAGLQLSLLRGTLDLHNLEIDNPEGFEHPTFLKLGHGHTALNIRSLLDETI
                     VIDRIQLENIEIVLEQKGLTSNLKEILNNLPKSEGQEPKAEEKKSKNIQITQLIIKDV
                     KVKANLVPVEGLARASTVSIPLKEIRMENIGTAEKIDAVQLTAMIIRAIAEGIVEQGK
                     GILPLDMINSFTEELSKQGEALLRTGQQLLEEAGKTGKELLDVPQDIGKKAAEGLKGL
                     LPGKKEE"
     gene            4552..5556
                     /locus_tag="PLE88_02045"
     CDS             4552..5556
                     /locus_tag="PLE88_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOM75278.1"
                     /translation="MQNQEQIRNGVILHPGAVGDCLLTLPLADYMKKVFGLDQMDFIG
                     RTEYIDFYPGRTCIDRIRSIESIELHRLFEDKNSFFLEDKDRLLEAFAGYEQIVSFLG
                     AGHPSFETNLLFTVHSTHSADVAVLSSAIPDDYTAHITDFYLQQFLNEHHLEQADAKI
                     ESDALIVPVASDYSAGQEMLEQIGIQHEQPIAVIHPGSGSPAKSWHWTNYLQLGSELK
                     ANNIQTVFILGPAEQDRMPEESAQIREKEIVLENLTLGQVVQLLTQADVFVGNDSGIS
                     HLSGMLGKRTIALFGPTNPAHFHPLGRSVTILTPNADSFSAFNLAECKKVFDSVIQML
                     "
     gene            5635..6669
                     /gene="lpxD"
                     /locus_tag="PLE88_02050"
     CDS             5635..6669
                     /gene="lpxD"
                     /locus_tag="PLE88_02050"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOM75279.1"
                     /translation="MSPKTIQEIAEHVGGRVIGQGDVLISSAATLDNAGPGQITFLSN
                     PKYTSRVKTTQAAGILVSKQVETDASQILVEDPYYAFMQAVVLLHGHRRHPQTGISEQ
                     ASIASSAHIGSNCNIHAFAVICENAVIGNNCQIYSGVFIGPNAVIGNDCVLYPNAVIY
                     DGCRLGNRVIVQSNASIGQDGFGFATHKGQHHKIPQIGIVILEDDVEIGSGCVVERGT
                     LDDTVIGKGSKLGDLVAIGHGTKIGPHCLLVPQAGVAGSAELGHHCVLGGQVGVVGHI
                     KVGNMVKIGAKAGVINDVPDGATLVGTPAIDANTAKRAYALIETLPQMRQKLRQLEKQ
                     IEGLTGGPKS"
     gene            6675..7532
                     /gene="lpxI"
                     /locus_tag="PLE88_02055"
     CDS             6675..7532
                     /gene="lpxI"
                     /locus_tag="PLE88_02055"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOM75280.1"
                     /translation="METKKILGLIAGQGRLPFLVADGARKAGLTVICVGLADNAEPQL
                     AEHADIFFYGSIARPGGWIRKLRKYGVRNAIMVGRVSKKQIYTPWRIVKYLPDWRALR
                     IWYWRLRKKDKRNHTVLCAIADEFASAGIFLEDSTMYCKEHLSDCGVMTACQPPAYAW
                     DDIDFGWPLLKKVSELDIGQAIAVREREIIAVEAIEGTARMIERAGQLCRKGGWTLLK
                     APRCHHDMRFDVPCIGPDTIESLKQNNACCVALEAGKTLILDKPQTLALADKLGIAVV
                     GLAGTERGH"
     gene            complement(7506..13013)
                     /locus_tag="PLE88_02060"
     CDS             complement(7506..13013)
                     /locus_tag="PLE88_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOM75281.1"
                     /translation="MDALIVFHYDASDSVKFTCSETSLQKLPSDQLLILTTALKEILS
                     LIKQESKTLYRMLINKRVQVRITDSAAGTASLLPHDILMNAALLTVEKRRLPHRHRML
                     MGILYRALYHWCNPELHITQVRMHTLRFYDAHRDILKSTIQEMQENAPLFDEPDWLET
                     LMQIEEIMLLDRFWTRLAKSPQTQLLLQEALRLPQTSARQKLRQILQSCVRTCQSDLL
                     IKATQEKILTGFKRFYTDSNSVVFVYELNPKLLKAVRICDSSKIDAMAASFACASIVF
                     KKVRTDIFHDHGRWIRGWLQERQAYSSDASLAVLEEMLLSDDLQKVRTAISQLAKKIR
                     RKDNAHQALRLLYAALYYWNHPDKGVCRSICLEVSGLLADILTERPFHFPPSRINRTV
                     FRTEPASVRICLPKARQTGRKIYKARILWYANGRRRNPILMEQDDAAEGSSMIAFSAV
                     FPVRTGWLHYAVQISDDNGASWQWEELDENSHGLIKFVPDERGQRVLSFYADTFNLQL
                     DDKYQPVKNEDGMYVYGTFYDIAEQLEAIRAEGYTRLYPLGALELGWAGEAGPDPSVF
                     SVWDGKTVRRDLGGIEALLYLRQKADQLGMKVLLCVLSHFSRANVSFAYHMPVYIADS
                     NGKLVRRAGWDGEWSEWLDSFMVNMRDFENVDALAEIGIELAQLGFGLRIDVGHGFDT
                     VFPISEQLEGTARLLGQVTVKGFEQVDLRKTEEPNIPLLYMCYRIQKAVPHATVIYAE
                     QWHGNEIRMIKSGTTPYNSLIKNLEHIRSGQPADAPLGLNDNLRYLNAIYKTYGGQTL
                     SLFNSHDEESPASNYQNMIWPAAAFLVLSSQGPLMYHISRLGGASAGSMQQRFDEAYT
                     ECWKHWVNNRFGHPWHEENKARWRILTAYPILYGFGKYLRGLFAFVEQYPALVRGEVI
                     PLNTQNGRIAAFLRAYKDHLFLCVFNFPASHAEGQQAVPRRFNFLLCNAFDRSPLEQI
                     RPDSIYEITERYNNTEGRSRRARKEFWWGNELIHLGFGGTLGPVSSHVFELIDKSNRV
                     SRHQMMLDSFVRYPLYGKQDRVCYTYVARIIAGIADEAPHSFEAFAEYFVLLATWIDH
                     KRKLGLNVLTSLLAEISEDNAQRRTAIIHFLMRIAVNEGNQFETAVCQSAADILHGMN
                     LGTIVLVSPESHYSGTAGGVGIYTTDIADVLSELGFHIVVVTPLYESLRDKILKTYTP
                     RYDGYSFAIHFPEFDEASQSIRTDTSPDIVNILRSQILRYKHGKHVRVEVLYLENGKY
                     LDAPYGGQTGEDKLRRARVLSQGALEALRAYNYYPAIIQMNEWPTWLVGAYLRRWTDY
                     NTDPHFDNTRVVGMMHNPHPSYSIRLAEANTFRRYYYCRVLGLEAHSDICLNPSGPFC
                     QQIDLTYLMLKTSDHIGTVSRAMKRRILDEPQVFGYADLFRQMEAEGRFFGRRNGFNM
                     AARQRFWFSCKKSILETYDRAARKRLFAKYTRAKLLAKRALQTDPAIRLTPDDDTHHH
                     VIFSMLHRISKQKGFELLVDWKAYQDKGNRWITYEPWKMMGPTVLEYFLNCDSRIQFV
                     ICGRVEDSWDGRRFDMHFRRIASMPEYRGRFAYYPEGTLPASLYRNVYVGSQFFVMPS
                     GGEVGEPCGISQQEAHAGGTPVIAHHQDGLIRTVCDADFGDTESPPNGIKFSGFNGES
                     LLTAMLDAVEIYFNGRRLHYLDKKGQPRKLRYSDLSYNAFTTDHRWLRLLRDYIQTYC
                     MMANVHLPDHIDAVRFAADAQGVSDHELPDLVLKKGMKVEYVIHCLAEALAAPVSSVR
                     TAAQNCLERLYRVLGDEVTAILKKRIDKNESSLEAEVLGRLLESMTTFGAG"
     gene            complement(13186..14475)
                     /locus_tag="PLE88_02065"
     CDS             complement(13186..14475)
                     /locus_tag="PLE88_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM75282.1"
                     /translation="MISEILALLQISLRSLWMHRLRSGLTTLGIVFGVASVIAMLAIG
                     EGASREAQEQISRLGSTNIILKTVKPPDIEVADASQQTIREYGLRYDDAERFRDTIPD
                     AQVVVPVRRLPQQTQYLNRRITAEAVGTVAWYPELAPIEVRRGRFLTPIDMHYSQAVC
                     VIDETVQELLFRYDDPIGQELKIAGEYYTVVGIVKDTSAKAEEPAAVSRTSQGGGVSG
                     YVYIPLATMKSRFGETQIQFGGTSGASGERIELSEITIKVPSMERVLPTRDVLASLLE
                     QYHKKKDYQIIVPLELLREAARTKRIFSIVLGSIAAISLLVGGIGIMNIMLATVSERT
                     REIGIRRALGARKKDIVIQFLSETILLTLIGGLLGMFVGTCIPILVTIFSNMPTVITL
                     QALLLSFGISAGIGLTFGLYPAYQAANMDPIESLRHE"
     gene            complement(14472..15206)
                     /locus_tag="PLE88_02070"
     CDS             complement(14472..15206)
                     /locus_tag="PLE88_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM75283.1"
                     /translation="MIRQNVPDSPPKGQPVVQIENLHKSYQIGALELPVLRGLDITFC
                     QGEYVAIMGPSGSGKSTLLNILGCLDKPTSGRYLLGSHDVSMMDDNQLSQVRGKQIGF
                     IFQSFNLIRQLNIIENIEVAMYYQRIPKSESRQRAIALAQRVGLGERLKHRPYELSGG
                     QQQRVAIARALANDPLIILADEPTGNLDTQSGREILVMLDELNAQGKTIIVVTHDEYV
                     AARTHRAIHLRDGRIEREVRNGKGGG"
     gene            complement(15203..17191)
                     /locus_tag="PLE88_02075"
     CDS             complement(15203..17191)
                     /locus_tag="PLE88_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOM75284.1"
                     /translation="MGITGRNRQKILLIAVVAGIVLAAYAAYRFRGGDSAVDSAMLST
                     YTAARGNLIMSVTESGTIKARNAMEIKSQVEGSTTIVSLVPEGTYITEEDVQNGRVLV
                     ELDSSELRDNVNQQEITFNSAEANYTEAKEALTIQKNQNDSDIQQGLMNVKFARMDLQ
                     KYLGEQMANRLIEQYKATGEMPVITDLIRDPNQLGGQSLQNYRKLTADIRLAEEEYRR
                     ANTDLGWTEKLFEKSYVSRSDLDADRLKVQRLKVSWEQAQTSLDLFEQYEFPKDAQKL
                     FSDYLEAERQLERIYAQARSRLAQAEARLNSSEATYLLNKERLERIRRQLEVCTVRAT
                     APGLVIYASSDRGGRYGGTRTYIEVGQAVRERELIMTVTNAAEKDVEVKVHETNVDKV
                     RVGQPVRIVVDAQPDKVFTGKVQKIAPLPDPQAFFGNPDLKVYSTVVSIENVSDQIKP
                     GMSARVEIIIAQLENVISIPVQCVATRGGRKVCYVMDGGQPKERPIQTGAFNDRFVEV
                     VDGLQEGQRVLLNPPRLLAEAAKSDEMQPVAAVSEQPADSSQSQPAATAADPQQRREF
                     MPDRSGSEEMPSGRRQMRREGGERAGNPADRFMEMDTNGDGKISLADEAPERARMFLQ
                     RLDTNQDGFVDAQEREAAASEMMRQGRRQPGGLGEQNP"
     gene            complement(17152..18783)
                     /locus_tag="PLE88_02080"
     CDS             complement(17152..18783)
                     /locus_tag="PLE88_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM75285.1"
                     /translation="MARKKRLYLRIGLLCLLSGLFGCKTAEDYKAKTDEEVYTILDEK
                     WRPEHGVKANYRIADVQPDPNDVIVDPNWMPSGRLSLAQAVAIATARSRTYQRNKEDL
                     YNSALSLSLERHNYVRQWFGTIDGGYARSASDESVDAGGRVGFNQLLADGTQIGSSLA
                     IDWMQFLTGSPRTSLGSVLSASISKPLMRGGGKEIVQENLTQSERRVLYQIRRFSRYR
                     KEFVVSIVRGYLQTLQSQDSVKNAQSNYASLRAAYEEASLKAQAGKLAQMQADQTEQR
                     MLQARDRLAEAQRSYQYALDNFKLTLALPVDADIELDPNELAGLKAMKIAEPNFPVDE
                     AVQLALQNRLDIATALDSVDDARRKVKVAADRLRARLDLNAGISNVRSPSSETDQWYR
                     LQFDEGTYSAGLTMDLPLDKKAEAAEYRQALISALQAQRDYEQTVDQVKLDVRNAYNQ
                     LIEAARRYEIQKISLELAQERVNSTTMLLQAGRAQSRDLLESQDSLLSAQNDTTSTLV
                     NYMLAKLNFYRDIELLNVKPDGLWESLEETDKKFF"
     gene            complement(18986..19468)
                     /locus_tag="PLE88_02085"
     CDS             complement(18986..19468)
                     /locus_tag="PLE88_02085"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOM75286.1"
                     /translation="MPYLFDGYNLLRAVQKMEEFASFSDVQLCRAISDFLSSIRQRGS
                     IIFDGIGPADKSAFEGMRYLEVHFSGSKKEADDIIEDMILDNTAPKRLVVVSSDRRLR
                     AAATKRKAAAVTATAFWIALLRQLEKAANKPMPEPSQKRGGLSKRETDIWLKIFGMNQ
                     "
     gene            complement(19472..20770)
                     /locus_tag="PLE88_02090"
     CDS             complement(19472..20770)
                     /locus_tag="PLE88_02090"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOM75287.1"
                     /translation="MAKMISETILIKNGRLIDPKNKVDRKADILLADGQVVKIGKNLT
                     ETADRLIDATDKIVAPGLIDLHVHFREPGDEEEETIASGSAAAVAGGFTSVVCMPNTA
                     PAIDEATAVEYVHRMGRQARKTYIYVMGAITKERAGKELAEMGLMAQAGAVGFTDDGS
                     GVQDTAVMLRAMKYASMFDCFIAQHCEDRSLARNGVMNAGYNATVLGLPGMDPLAEEL
                     MLWRDIQLVKKTGVRYHAQHISTAGSVEIIRQAKAQGLPVTCEVTPHHLLLTEEAVVN
                     YDPYYKVNPPLRTQKDIEAIRQAVADGVIDALATDHAPHLKSEKELEFLTAPCGIAGI
                     ECALPLYRKALIDTGVCGWPQMLSMLTYKPASILNVAKGSLAVGDRADAVIIDPDAQW
                     TVDSSKFYSKGRNCPYQGWTVKTKVLYTIVAGEIRFAAED"
     gene            complement(20770..21738)
                     /locus_tag="PLE88_02095"
     CDS             complement(20770..21738)
                     /locus_tag="PLE88_02095"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOM75288.1"
                     /translation="MDTQTKTEKFQWTRKHLIGLSDLSRREIEFILDTAQSFEEFSTR
                     SIKKAPPLRGKVVVNMFFEDSTRTRNSFTLAASRLSADVIEFTEKSSSVSKGETLIDT
                     ARNLEAMGVDIVVIRHSAGGAPKLLTRCINACVVNAGDGYHEHPTQALLDVYTIRQVR
                     GSLDGLKIGIVGDIAHSRVARSNIYAMTKLGAEVTLVGPPTLMPARVQDLPVKVSYNL
                     DAVIEDFDVINMLRIQFERLGGNPFPSVREYSHFFGLTVERLKRAKPDILVMHPGPMN
                     RGVEIESEVADGPNSVILRQVANGLAVRMAVLFLVNQAAAQEQKEK"
     gene            complement(21738..22262)
                     /gene="pyrR"
                     /locus_tag="PLE88_02100"
     CDS             complement(21738..22262)
                     /gene="pyrR"
                     /locus_tag="PLE88_02100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOM75289.1"
                     /translation="MKTLLDGKQLNEAIEGIVRQIKSDIPADTDIAVIGIRSRGEILA
                     KRLVKALSSYFTGQILNGTLDITLYRDDLNEPKEVQPTVRSTEINFDITDKLILLADD
                     VLHTGRSVRAAMDALTDLGRPRMVRLAVLVDRGHHEYPICADYTGLKTDVQEPQVVRV
                     HFTETDNEDRVIIE"
     gene            22568..24295
                     /locus_tag="PLE88_02105"
     CDS             22568..24295
                     /locus_tag="PLE88_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOM75290.1"
                     /translation="MASGESNPSMLPVEPAALDYARIYTVVQYDPSLTGRGIRIAYIC
                     PSETYINNRPQNDYRINVHHSCLYDADVAFEDGSDGKWGLSSHETACAGILLGADEKA
                     FIPDFGMFQYRGACPDASVDVYEFWRFAALYLFGKKPFEADIVTLSLGNIFEEWWTRG
                     LEQAADEKDLLVIAGIGNGAGVHTPKPLYPAAGANILGVGVVDAAVDQHGTISLWDFS
                     SAKAVHSSVGPTEDGRCKPDIIAPGTALVPSHTDNTGYVLASNWSSLSAPIVSGTAAL
                     LLQKAAMFEQNDIYNQPGKALLLKAILMNSARKLPFWHKGRVEAEDDPETPLDYIQGA
                     GVIDGLEAYQQLTAGPGSPGSVSPVGWDNRMLDNTHTSCDYMFTAAEPNQFITATLCW
                     NRVYQPHYPFNRLTEQDTDLRLELWAVNPNEPNQPILLDYSDSINDNIEHIYAACSPN
                     YTVYFLRVRFNDLQTMQNPVRQRFAVAWSVGPDRQAGNPWWYDLNGDDQIDATDQLIY
                     AMLDSGMFTDADAVSLGQALNCTPERINLLVGGWDVWKRYLTNWELASDTPPAVDAQN
                     LPSDVGIIQ"
     gene            complement(24233..24985)
                     /locus_tag="PLE88_02110"
     CDS             complement(24233..24985)
                     /locus_tag="PLE88_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM75291.1"
                     /translation="MEYDNAAAIITGASGKLGREIAVFLGRKGFHCLCHYGTNAAAAQ
                     QAAETIRQYGRKALAVACDLEEPDCADFLIEQARRLGPIRLVVHSASVFQRQPAETLT
                     PLQVSRMLAVNLAAPLSISNVFVRYLKQEGLDWKNAVQPFAAIIHMVDIGGVKPWAEY
                     APYSAARAGLIAAAKALAKELAPGITVNAVAPGIVTWPDKMDPAEEEKQLKLIPAGRF
                     GTPADVTGAIGFLLDNSYITGQVLCVDGGRSI"
     gene            25112..25834
                     /locus_tag="PLE88_02115"
     CDS             25112..25834
                     /locus_tag="PLE88_02115"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOM75292.1"
                     /translation="MALLNVNIDHIATIRQARRTDEPDPVWAAVQCQLAGANGITVHL
                     RQDRRHINDRDVRVLKETVACKFNLEMCLSESIVRIAEAVIPDQVTLVPENRAELTTE
                     GGLDCAAVKQRLAAVVKRMHKKGILVSTFILPDERQILASAEAGCDAVELHTGRYANA
                     VSDKDIEKQLNLLRDGRDLAAAAGLIVHAGHGLTYRNIRPVANIEGLCEFNIGHSIVS
                     RAVLVGLHQAVREMKQLIENPF"
     gene            25847..26722
                     /gene="dapA"
                     /locus_tag="PLE88_02120"
     CDS             25847..26722
                     /gene="dapA"
                     /locus_tag="PLE88_02120"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOM75293.1"
                     /translation="MFSGALVAIITPFSEGQVDFETLTELVEFQLESGIDGIVPVGTT
                     GESPTLSYDEHKRVIEHVVKTVGRQVPVIAGTGSNSTAEAIELTEFARKVGADASLQV
                     APYYNKPMQEGFYQHFKAIAEEVDLPMVLYNIPGRCGGTGLTAETVARLAEVDNIVAI
                     KEATGSMDMASEIASRCDITILSGDDSLTLPLAAIGAKGVISVVANIVPADVKAMTDL
                     ILEGDFVSARKWHRKLFPLSKTMLSLATNPIPVKAALAMLNMASEELRLPMTVLEEAK
                     KAQLRQLLKDYGLLD"
     gene            complement(26777..27397)
                     /locus_tag="PLE88_02125"
     CDS             complement(26777..27397)
                     /locus_tag="PLE88_02125"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOM75294.1"
                     /translation="MDEKTSPSAYPAVLLAVLGAVFLWSAVKPADRFTWVLEMFPVVL
                     ALLVLIPTCRRFPLTHLSYTLILLHAVILLVGAHYTYAQVPLFNWIKSTFDLSRNHYD
                     RIGHLAQGFVPAIVGRELLIRTSPLKNSKWLAPVVLAFCLSISALYELLEWTVAELSG
                     TAAEAFLGTQGDVWDTQKDMLFCLVGAAVSLLVLSRWHSRQLRRLE"
     gene            complement(27390..28319)
                     /locus_tag="PLE88_02130"
     CDS             complement(27390..28319)
                     /locus_tag="PLE88_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOM75295.1"
                     /translation="MADLKRGNSAICAVTYWGMVINIVLSVLKCVVGIAVASLSLFAD
                     GIHSLSDCLTDIAVLVGIHLGAKEADSEHPFGHGRQETFATAVIALVLIAVGAGMIYK
                     ASMDIAKMHYTIGSQPVTISSSVIWIALLSVITKEALYQWTRIIAVRTHSSILYANAW
                     HHRSDAFSSVAVLFGAVSVKLGYPYGDQLAAIVVGLMIIFVGGKVISDCLGEFAERAV
                     DKQTVRQIQTIIESDRKILNWHQLRTRRVGREIFLDLHILVNPELSIAEAHEISEGLE
                     QALRDQLTQPVNVIVHIEPDLPHLRRERKTADG"
     gene            complement(28321..29697)
                     /gene="glmM"
                     /locus_tag="PLE88_02135"
     CDS             complement(28321..29697)
                     /gene="glmM"
                     /locus_tag="PLE88_02135"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOM75296.1"
                     /translation="MAQELIISISGMRGLVGHNLFPNTAADYGSAFGTFLKRQCSSSG
                     RCAVAIGRDSRPSGQMLFSAVASGLTAAGADVIDLGICSTPGVGIMVRHLGCAGGVVI
                     TASHNPAPYNGIKLLLGNGIAPPKPTAEQIIQLFRSQQFERTDALGCGTICQNRDTHQ
                     VHLEKVLAIVQKESIIPKHFRVVLDSVNGAGGTAGQMLLEALGCQVICMNAEPTGRFA
                     HAPEPTAENLTALCRQVAAEKADIGFAQDPDADRLAIVDEKGVYIGEEFTLALAAKQV
                     FSRKKGAAAANLSTSRMIDDIASAAGCRVIRTPVGEAHVANAMIEHNCVIGGEGNGGI
                     IDLRVGPVRDSLVGMALVLQLMADAGKSVSQLAAEVGGYCMYKTKYSADTIQAQAVIE
                     KAKSLFADALINTRDGCRLDLPDGWIHIRTSNTEPIMRVIIEAKEAAAADRYAKQIER
                     ICGEVIGK"
     gene            complement(29716..31560)
                     /locus_tag="PLE88_02140"
     CDS             complement(29716..31560)
                     /locus_tag="PLE88_02140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOM75297.1"
                     /translation="MTSRKTKQQTQMRFEFGTADSGPGQGQAHPSGMKSNRKRTENAE
                     SSDALENLNTLQTPAAGSSAKAGENAAGSTSAAAAAPKKVRKSQAATAESMAAKQREI
                     SVSEFFAKNRHLLGFDNPRKALLTAVKEAVDNSLDACEEAQILPAISVHIEALDGTDN
                     KFRVTVKDNGPGIVPKQIPNIFARLLYGSKFHTLKMSRGQQGIGISAAGMYGLLTTGE
                     PVQIISRTSGSKPAKHYHVQIDTSKNRPEVILDEECEFDQPSGTSVSIVLEGRYQKGR
                     QSVDEYLAQTAMANPHASITYRAPDGTVSQYPRQTEEMPYIPAEIKPHPYGVELGILY
                     KMARDSECRRLADFMHREFSRVSERLAAEIIKKAKLSVNMKPAAITLKDAEKLHAAIN
                     ATKLMAPPTDCISPIGQEKLIDGLKRVTEAEFYVSCTRKPAVYRGNPFIIEAALAYGC
                     KEMKDEDQEEGKEPLMRLVRFANRVPLLYQQSACATYKAVMETNWRAYGLNQSKGALP
                     SGPVMLMVHIASVWVPFTSESKEAVAHYPEIIKEIKLAIQECGRKLGLHVRRQRRMKE
                     ELLKRSYIETYLPHIGQALKDILLLKEAQVEKLLNNLSTILERTRSLP"
     gene            complement(31643..33232)
                     /gene="serA"
                     /locus_tag="PLE88_02145"
     CDS             complement(31643..33232)
                     /gene="serA"
                     /locus_tag="PLE88_02145"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOM75298.1"
                     /translation="MYKILITDKLAKEGIDLIKSMDDFEPVVRTGISEDELAQIIGDY
                     DGLIIRSGTQVTAKVLENPGKLKGIARAGVGIDNVDVPAATRKGILVMNTPGGNTISA
                     AEHTMALMLALSRNVVPACNSLKAGTWDRKSYTGNQLNNKVLGVIGLGRIGMAVVKMA
                     LGFNMKIIGYDPFAAPKEAEQLGVQITDNIEKIYREADYITLHIPRNEQTLNLITSKQ
                     LKMMKPTCRIINCARGGIINEDDLYNALSAGTIAGAALDVFSKEPPENKRFAECPTCL
                     VTPHLGASTEEAQIEVAVEAAQILCDAIKGGPIRNALNAPATSGAVPPIVSRYADLAQ
                     RIGTMLSTLAPGHLKSAKIEFRGSIAEKPVDAAATAFTIGLLQPHFDTVVNMVNAPLL
                     AKERGVSIDRTKNPEIKDLESSFTATVVTDKITRSIRGTVFSGNLLRIIEIDGFSIEV
                     TPEGTMVIIFNQDKPGVIGAVGTALGRHGININTMGVGHKLEEGKAILAVSLDKTPDA
                     QAAQELAGLEFVNEMYVCKLQ"
     gene            complement(33244..33945)
                     /locus_tag="PLE88_02150"
     CDS             complement(33244..33945)
                     /locus_tag="PLE88_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75299.1"
                     /translation="MKTLNLKYSVRFIARFFVFVVLLSNVFVLSGCASGLGLLFSPGA
                     FEKKTTPDYNLKEQEKRKILVWIECPRSAGADYGFPQQLASAFHLYMTAKAGFNSSNI
                     ILNPPAPSQAAGLNPQEIARSQGAGYVLIVHVDKFEAEALGIRSYCSGRLIIRAALLD
                     TDLGVAVWPSQPEGKMIDLAVEMETGGRANLVSRLTAGAAHCTLRYLYPCDKLKFKCA
                     DERVSVQEAFETETF"
     gene            complement(33926..34381)
                     /locus_tag="PLE88_02155"
     CDS             complement(33926..34381)
                     /locus_tag="PLE88_02155"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOM75300.1"
                     /translation="MQFTENNRRLSGRMRLLAAGIFVAAAVVFGLLWAAAEGYVDLSR
                     WVGICGFKQRYGLPCPGCGWTHAAQAFAQGHPIEAFKIQPAACIFCLTAAITAFFALL
                     YALFGIKFLPLERLFSSTTIRNLIIAGILVIMTGWIVTLIRTVLENAKP"
     gene            34452..35267
                     /locus_tag="PLE88_02160"
     CDS             34452..35267
                     /locus_tag="PLE88_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOM75301.1"
                     /translation="MSIPHTDLSQMLETAVVAARLAGQRAMEELRYIRKLPKSDNELV
                     TQADPVCQKIIIDCIREHYPDHGFLCEEGPNGQLYRIQPRGEEAVWWIIDPIDGTNNF
                     ANGLLCFCVSIAAMLEGQPLLGVVFDPTTDSMYSAAQDMDALLNGSRIVVSDDAIGPF
                     ASFAIDSHTAPQTDSAVKQIMQSTRFRCLGSTALHMAYVAKGAMIGMFSSSARLWDIA
                     AGAILVERAGGRVSDLKGNPLFPVNPQLYNMERLPTLAASAKIYPKALELFAG"
     gene            35356..37692
                     /locus_tag="PLE88_02165"
     CDS             35356..37692
                     /locus_tag="PLE88_02165"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOM75302.1"
                     /translation="MNTKRLFIEVLLASFIFFACLPAQAANGSDDVLLNTLPDNCLFC
                     LRVNAFSTTLSKLDMYLAGTSPIPMSIAMLANMQLIGITGDPMLGGIDQNGDFAVFGL
                     LNPDAAAGVPVIGGVLIPVTNYADFVKNNPNCKAGDSGVTVLSAPNSQIGSLMLTEAA
                     GGKYALAVMEGQRDTLGTLKTALSAPAKPLAARLRPAHSQTAAAAPAWGYINLSLLYN
                     TYQPMVLNMLQQAQSQIPAASEEMAGMTAFVLKMYAELFKEFAKDADYAAVTLTPEPA
                     LLCIDTALQTKDGSQLAGILTASPQPAKGFTMAGYLDDASAINGLAKLNLASVQKMYD
                     KIFDILAAASEQTAMKDQMETMKAMLKKSIAAMGEEVAFSFSYAGSKPPVKLREIVAV
                     RDSAAMKELMSENVACANLIYQAMGFPAVFKYQPNVSTYKGISIDAASIEMKLPQDPN
                     NPMNAAIEQMYGDAFAYHMALTPDKFLMTMGTDSQETLQKMLDQPAAAAPAGDFKMAW
                     EMLQNTPYSECVCSINIIKLLTGLGEMMQSVEPAASTPIPDIFGGLKGMQTQSCLALG
                     GFSADGQLAIRAAFPKQHLTEIISAVMKMQQKGIEAQQAAIKPGVQTAAPAVPEAPAS
                     QKRPAADDASETAAILKSWIGKPAPELRMVDLEGKIYRVSRLKGKKVLLDFWATWCPP
                     CKESIPFLVELRGKTSDADLVIFGLSDEPTDRLNQFVKDFKINYPIISYGDNLPAPYS
                     QITALPTVFVIDTKGIIRDIIVGFEKPQDILTALNKIS"
     gene            complement(37780..38787)
                     /locus_tag="PLE88_02170"
     CDS             complement(37780..38787)
                     /locus_tag="PLE88_02170"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75303.1"
                     /translation="MTLTDFEMDVLRWIQRPLPVCSRPFAVLAERLSATQEQVIRTIR
                     QLQQQRLIRRIRAQIDYRALGRTASLIAAHVPAERFSEVADAVSRLPAVSHSYCRDHY
                     YNLWFTMQAPSLIAVDAELAGLQEQLQVEFHSLPAQRLFKLDVYFEPAGPFASVSDPM
                     PQPVQDIALSDIPRQPAELSDSERHTLHLIQQDLPVAEYAFAVLAGQDGTITEADFLA
                     ALQSLLQKGVVRKIAAVVNYPALGYAANALFCAHVAQDSIRTAGQALALCPWVSHCYQ
                     RRSVEGWPFSLYAMGHAGSREQIADLVRLFCEANGICRWQILPTVSELKKEPVKIQLP
                     R"
     gene            38939..40387
                     /gene="ahcY"
                     /locus_tag="PLE88_02175"
     CDS             38939..40387
                     /gene="ahcY"
                     /locus_tag="PLE88_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOM75304.1"
                     /translation="MKTVQNTSTKTKMLDLSLKYKVADLSLAEFGRKEMQLAEKEMPG
                     LMATREKYGTEKPLKGLKITGSLHMTIQTAMLIETLEILGADVRWASCNIFSTQDHAA
                     AAIAAAGRSAVFAWKGETLEDYWWCTEQALTWPDGSGPDLIVDDGGDATLMVIQGMRV
                     EKDPSLLAKTYDNKEFQIIMDRLAASYSANPRRWTTVAQNLKGVSEETTTGVHRLYQL
                     QESGELPFPAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMVAGKVVVVCGYGDVG
                     KGCAQSMRALGARVLITEIDPICALQALMEGFQVTTMEEAAPQGDIFITATGCCDVIR
                     AEHMEQMKDEAILCNIGHFDSEIDMHYLEHSPLCKKTNIKPQVDKWTLPSGRSLIVLA
                     EGRLVNLGCATGHPSFVMSSSFTNQCLAQIMLATESLEPGVYTLPKKLDEEVARLHVK
                     ALGGKLTHLTEKQAGYLGVPVNGPFKPDHYRY"
     gene            40667..43249
                     /gene="clpB"
                     /locus_tag="PLE88_02180"
     CDS             40667..43249
                     /gene="clpB"
                     /locus_tag="PLE88_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOM75305.1"
                     /translation="MKFDKFTVKAQEAIATSQQLALAGRHTVVTPLHLLSALLDDADG
                     MAVEILKNIGTNVERIRQMTESELNRQPKGQVNGMIMPDAAFSQVVLDAQNKADKMGD
                     EFLSIEHLLMALADVKSDAKEILSVNSISVEAITKALQNLRGDAKVTDDNPESKYKAL
                     ERYGIDLIAQAQKGKLDPVIGRDDEIRRCMQVLNRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVAGDVPSGLKNKKIIALDMGALIAGTKFRGEFEDRFKAVLKEVIQSDGQIILFI
                     DELHTIVGTGKTEGSVDAGNLLKPSLARGDLRCIGATTLDEYRKYIEKDAALERRFQP
                     VMVDPPTVEETIAILRGLKDRYDAHHGVRITDAALVAAAALSNRYISDRFLPDKAIDL
                     MDEAASRLRIENDSLPSELDSIRREIMRLEIEREALKKETDAASRKQLEKCEKQLAEL
                     KTQNAQLTARWENEKGAIDKVKALKEKITAVQSQFEEAQRRGDLERAARLKYETLLGL
                     QKELKTAEESVAKANGSAMIHNEVTEEHIAQVVSTWTGIPVSRLMSGQRERLMKMEEE
                     IRRRVVGQDEAVAAVCNAVRRSRAGLGDPHRPIGTFLFLGPTGVGKTELSKALSEFMF
                     NDPNSMVRIDMSEFMEQHSVARLIGAPPGYVGYEEGGRLTEAVRRKPYSVVLLDEIEK
                     AHPDVFNVLLQVFDDGRLTDGKGRTVDFKNTVIIMTSNFASQQILQLSEQNGADWEIE
                     AHVKEALRNYFKPEFLNRIDEIIIFHTLKREHLEQIVDIQLGYLISRLKERQFAAEVT
                     AAARKLLMDEGYDPSFGARPLKRVIQQRIENELAKEIIAGKFAEGDTIRIDADAHGFK
                     FVKV"
     gene            43251..43766
                     /locus_tag="PLE88_02185"
     CDS             43251..43766
                     /locus_tag="PLE88_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HOM75306.1"
                     /translation="MLTVWLVLLILVNAGWLATVLFMLPGNWLMVVSTCLFAWWKWDT
                     GVFSWPLLAGITVLALAGELIEFFAGAGGAKKAGAGWTGAAWAVVGAVAGAVAGTAVI
                     PVPLFGTLLGACLGAGLLTWAAERTAGRPKQHSVRSGVGAGTGVLVGIAAKFAVGCLI
                     WLMIAVAAFWP"
     gene            43903..44535
                     /locus_tag="PLE88_02190"
     CDS             43903..44535
                     /locus_tag="PLE88_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOM75307.1"
                     /translation="MKTVGLACAVVLTSGVLWADPETYGDVSVSALLRIDETVTLFCD
                     IADFPPVIGQNMPVQIRGLKTAASGQDNLPLLAFLNTFFFSGSVQHTLVLRNIQRGQQ
                     FCLVADIEVDGQDLCQLLVEKKLAQRIIEIPSAAGSRPDAAGASAGLQNPPSASQPSP
                     AGYIATKTSKVFHRADCPHIKRLDASKTVVFPTRQNAAASGRRPCKTCNP"
     gene            complement(44536..45249)
                     /locus_tag="PLE88_02195"
     CDS             complement(44536..45249)
                     /locus_tag="PLE88_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOM75308.1"
                     /translation="MNPMALLATVAEESAGRSAGLFDVVQAKQELWDSISRLDWRFAL
                     LMISVGVVYTLYGWRIFRVLVVISFGFIGLFLGILAGNKLNSEAYNPVLWGGIIGMVL
                     FAAVSMPLMKWCVSVLGALAGGIITGGLWIAFGLSQTYLPAGFIVGFIAGGLISFILL
                     KVSVMLFTSLGGSLIMMTGMLTLVYQYEKYVMNPPTQHLNAFFFQHDWFLPLVLIIPT
                     VIGMLIQNKLIKHSPNWEI"
     gene            complement(45625..46635)
                     /locus_tag="PLE88_02200"
     CDS             complement(45625..46635)
                     /locus_tag="PLE88_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75309.1"
                     /translation="MKHLKWMAAAAVAAALSMPLMAAEQADPAVGDWLLKADFDGRPM
                     NAILIIAKDKDGKLTGLWGSFFGTDAVDNLKAADGKISFSQTIRFRPPGGGQDQSEGF
                     TVDYSGTIKEGKMSLTASSDRGDFVYEGRRLDALPPVLGVWEFKRQRNEQEIITLLTV
                     SQAADGTLTADWTGPANSQMQAESKISDVKFENGKLTFTRSLSFQDRQMETTYELTAS
                     GDTLSGVSKSQRGEREVTGRRLGSELIGKWDLSVSSDFGQRRQLLIVLPDMTGMYGAV
                     NVGKITFENGQVSFKYSMGFGDRTFESEFKGQLQDGKLTGQITTSRGTQQVEGKKMMP
                     AA"
     gene            complement(46703..48148)
                     /locus_tag="PLE88_02205"
     CDS             complement(46703..48148)
                     /locus_tag="PLE88_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM75310.1"
                     /translation="MNMRRREFLKGSAAVALGLSAASGSRILGANDVIRVGIIGVGRQ
                     GSYHAQRLAKMPGVQLAALADPDPGYKMGQLKDELAHSEDNPLEVEIYTDYRKLLDRK
                     DIDAVVIVSCNHWHALHSIHTLQAGKHVYVEKPVSHDVWQGRQLVNLAGKSRLVVQAG
                     LHHRSRQCWQEAMEYLKQGHLGKVLCVRGLCYKERGDIGKLDTPAAPPATCDYQMWLG
                     PAQDEPIWRPQFHYDWHWVWNTGDGDLGNQGVHQVDIGRWMLGQTGYPDHILSIGGRF
                     VYSDAGQTPNTQILFYDYKPVPLIFEVRGLPAQPGIRGMSIYKKTRIGNVIECEGGYI
                     AEETVYDNDGRRIHRFSSGGGELHLTRFIDAVRSGQADAVPCSIADGHISSALCHLAN
                     ISHRLGRQAPPEAIAQQVKQDALLADAWQRFAEHLEANGVDLKQTQAVLGAALTFDGR
                     QEQFTGPLAPTANGYLKNNYRDGWIIPEIKV"
     gene            48366..50957
                     /gene="lon"
                     /locus_tag="PLE88_02210"
     CDS             48366..50957
                     /gene="lon"
                     /locus_tag="PLE88_02210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOM75311.1"
                     /translation="MRNFKKAQTSLFLLAGQSGKIAGRYSRDKKTVLKGFATCMRLME
                     LIELEPKNDAAPSAGNGRNAEYSLPEEIGILPIRSAVAYPGTVMPLAVGRERSKRLIT
                     DIAPQETIFGLLTQKNPDIEDPAPTDLYTVGTAASVLKVIRMPQGSVNIVVHGMMRFK
                     VLRFTATEPYLKARVEPLPVQVKNTKKLQALMVSVRNTANRVIALSPNVPEEAALLLE
                     NIADPSALADFLAATLNISIAEKQALLEETDAYKRLEKISLALASQLEVLELSHKIQG
                     QVRESIDKSQREYFLQEEMKAIQAELGQDDRRTQELKELQEKIEAAHMPPAVEAEARR
                     ELDRLGKTPPGSPDYGVIRTYLDWMCELPWAVQTDDRISIARARRILNQDHYGLDKVK
                     RRILEFLAVRKLNPRGKAAILCFVGPPGVGKTSLGKSIARSMGRKFVRISLGGIRDEA
                     DIRGHRRTYIGALPGRILQELRKCGSKNPVFMLDELDKIGQDFRGDPASALLEVLDPE
                     QNNTFTDHYLDLPFDLSNVMFIGTANYTEPIPPALMDRMEILQLAGYTKNEKLHIAKK
                     YLIARQLKEHGLTPSQLTISDEALGVMIDSYTREAGVRNLERTIGSICRYVATQIAEG
                     KRKQAAIRTQDLLTILGPPQFEPEVALRTAVAGVATGLAYTPAGGQILFVESVKMPGK
                     GQLQLTGQIGEVMKESAQAAFSVLRSHAEALAIEPKVFSKYDFHIHVPAGAVPKDGPS
                     AGVAIFTSLASLLKGIPVRPDVAMTGEITLQGLLLPIGGLKEKILAAKQAGIKTVILP
                     QRNKKDMLEIPREAKSGMQFVYARSAAEVLKTALAAVVPQGGGQVPARRRQKKLQGKK
                     TALPSKK"
     gene            51002..51075
                     /locus_tag="PLE88_02215"
     tRNA            51002..51075
                     /locus_tag="PLE88_02215"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51036..51038,aa:Asp,seq:gtc)
     gene            51049..>51280
                     /locus_tag="PLE88_02220"
     CDS             51049..>51280
                     /locus_tag="PLE88_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75312.1"
                     /translation="MRVRAPSASFAAGLPLKSHRLRRAWQYAFYFFSLLTGKFLRRLR
                     GAKPAGDFLHPAAGKRDLSLPVPSETCEYLRLW"
BASE COUNT        12285 a        13973 c        13196 g        11826 t
ORIGIN      
        1 cggttcagcc ggctatcggg gttttgttac agattgcgca cgccgatggc tgcaaaatca
       61 tacttgcgat cctgcctcaa cagcaatctt cgcctgcggg ccggaagcta tgctgaaaag
      121 cacagcgcgt ctggcaggag atttttcgct gccctgtcaa atcagtatgg aaaggatgat
      181 ggcctgcggc atcggcttat gccaaagctg tgccgttgaa gtaaaaaatg cagagacacg
      241 ctatcaatta tgctgtaagg acggcccggt cttcgatgcc cgcaatgtta tatttcaggc
      301 agactgatgg aagcagatac tacattaaaa acagaatttg ccggacttga attggcaagt
      361 ccggtattcc tggcatctgg aacctgcggt tatgccgatg aacttgccga gttcatggat
      421 ttatcaattc ttggcggcat aaccacaaaa agcatcacac ttgaatcccg taaaggaaat
      481 ccgacacccc ggatagtcga aacctgcgga ggcatgctca acgccatcgg tttggctaat
      541 atgggcttgg aacagttcca aaaccagatg cttcctgtat tggagaaact gcccactcgc
      601 gtttttgtga acttggcagg aaaaacgatt gaagagtatg cggctgtcgc ccgggcacta
      661 tctgccgaaa cagctatcga cggtttcgaa ttgaatatca gctgtccaaa tgtcaaaaaa
      721 ggcggcatta cattcggcac cgatcccgtg cttattcggg agattacagc cgctgttaaa
      781 aaggaatgcg gcaaaaaacc gcttatggtc aaactgactc cgatggtgac aaatattacc
      841 gcaaccgcga ctgctgccat tgaaggcgga gccgatgcat taagcatgat aaacaccttt
      901 actgcaatgg ttatcgacat tcatcaacgc aggccggtct tggccaatcg gacaggcggg
      961 ctcagcggac ccgcaatcaa gcccatagca gtgtacttag tcagcaaagt ctatcaagag
     1021 gcggccaaag atgccggcat tcctatttta gggatgggag ggataatgac ggcatctgac
     1081 gctctggaat ttttcattgc aggcgcttct gccgtcgcta tagggacagc aacattcatt
     1141 taccccgata cagcccgacg ggtaattgaa ggattgcggt gttattgcac ccaaaaagga
     1201 ttctcccgca tttcagatct tgtcggcacc ctgcaataaa aagaggcact ttacagaatt
     1261 gttttctaaa cctatattta tttcagtata acagtatcaa aatgccgaaa atgagtaaag
     1321 agtgctatga gccaatcaga aatactaaaa ttgttaatgg actattttcc aacggcggtt
     1381 aataccggac gaagtcttgt ttttatcagc gaagactggc gaattgaact gattgagaga
     1441 aaaaatccct ttttcggaca gaatattacg aatactccta ttatacgcgt taaagttttt
     1501 aaaaaagccc ttaacggcga atttcttgcc ggccattatg aagattttca aattctttca
     1561 attaatgacc ttgccttaca gattgaacga tatattcaaa acgctgtagg cacaaatatt
     1621 cgggaaaatg cataatctgc tgtccgctat ctgcagagaa catcctcaag gggtggagat
     1681 aacagtcaag gccgttgccg gcagcagccg aacggcaatt gccggaatcc acggccaaat
     1741 gctgaaaatc aaagttgccg cacctccgga aaaaggaaag gccaacaagg caattgttga
     1801 acttttggca aataaattga aactgaaaaa gtctgacgta ctgattcaaa gcggccatac
     1861 aagctgtgtc aaacaggttt tatttcaagg gattacagtg catgatgtgg aaatcctgtt
     1921 taacgaaaaa acctgaatct cgggattttt ctgccggatt ctgtatgctt gcggcggcat
     1981 ctttgctgct gagcctgcag gcagatggag tcactctggc aaattcacaa aaacaggggc
     2041 ttcgggctta cacgatcgag ggcatgctga aactccctga caatgagatt gacatcggca
     2101 cagccgcatt gattttaagc cgccaatggg gaagccagcg aacactctat gtatatcgtc
     2161 gaaaaattga tgatatggct gaggccattc taaaaaaact gcatgaacag catctgcccg
     2221 ctgaccatcg tgctgtcagc gtcatgaata cctacttgtt tgaggaactc gggttctcat
     2281 ctgtcgaaac ggcagacaat ccggaggatt tattcctgca tactgttctg gataaaaaaa
     2341 ggggatattg cctgagtctt tctattttat atcttgcagt tgccgagcgg ctggggcttc
     2401 ctgtgtacgg agtggttgtt ccggggcatt ttttcgttcg gtacgatgac ggccgccatc
     2461 ggatgaatat tgaaaccaca tccaaagggg ctggcgctga tgatgcgcac tatatcgcca
     2521 cattcaaacc gcctgccaat ccccagatgc tctatatgaa aaatctgact cccaaacaaa
     2581 ctctgggatg ctttttcaac aatttaggca acagctatac agccgttgga gataccgaga
     2641 aggccttcga atcgcttctg cgtgctgttc aaattaatcc ccttctgtca gaagcgaata
     2701 tgaatctggg aaatatttat ctccagaaaa aaatgccgca ccaagccatt gcccaatacg
     2761 aaaaggcctt gtcaattctc ggaagcgacg ccaaagtgat gaataacctc ggttctgctt
     2821 atatgcagtt atccaattac cctcgtgccg aatcctatta caagaccgcc ttatcactcg
     2881 atccgcaata tacagatgtt tatcgaaatt tggcccaagc gctgcaaatg cagggaaaat
     2941 atgaagaagc tgtcagccag cttcaggcag ctgttgtcct gaatccgaag gatgcggcag
     3001 cgtttgctct gctaggacag atttgccgcc aactccagcg tttcccggat gcagaaaaat
     3061 acctctcgaa ggctttgtcg ctggatgcat cgctggcatc cgtacggatt tccctcggct
     3121 atctctattt agaccagagc cggctggaac aggcgcagga ggagttttca tctgcccttc
     3181 agtcagccga caccgccgcg caggcctatt tcggcttagc gcagatttac catcaaaagg
     3241 gacaggctga ggatgaaatc gctgcctacg agcaagccct cgctatcgac ccgtcgatga
     3301 cggctgcgct gcagaacctt ggcaatgcct acagccgaaa aggcaatgac atggcggcaa
     3361 ttgacgccta taagaaagcg attcgcatta atcctaaaaa tccggatctg cactacaatc
     3421 tgggcattgc gctggccaaa atgcaacatc atgagcaggc catagcggct tttttggaag
     3481 caatccagct ggacgccaac aatgccgccg cttataatgg attagcaatt tcgtattacc
     3541 agtccggcaa taaagagctg gctcgaatct atgcccagaa agcaaaatcg ctgggattcg
     3601 aaatttctga tatattgctg aaataaaatt atgaaaggaa aaatatgaat aaacagcaga
     3661 aaattctgac gggtatcgtt tttatcctgt ttgtacttat tgttgccgcc ggcatcctga
     3721 taaaaatata cggcaatgaa gcattgcgca tcggaataga aaaggcatcc caaagcgcat
     3781 tacaagtcaa aacgcgtctg gctgggcttc aattgtcgct gctccgcgga acgttggatt
     3841 tgcataatct ggaaatcgat aatcccgaag gctttgagca ccctaccttc ctcaagcttg
     3901 ggcatggcca caccgcttta aacattcgca gtcttctcga tgaaacaatt gtaatcgata
     3961 ggattcagct tgaaaacatc gaaatcgtac ttgaacaaaa aggcctgacc agcaacctga
     4021 aagagatact caataatctg cctaaaagcg aaggtcaaga accaaaagca gaagaaaaaa
     4081 aatccaaaaa cattcaaatt acccaactga ttatcaagga tgtcaaagtc aaggccaatt
     4141 tagtgccggt ggaagggctt gcgcgggctt cgacggtcag tatccctctc aaggaaatcc
     4201 gtatggaaaa tatcggcaca gcagagaaga tagatgccgt tcagctgacc gccatgatta
     4261 ttcgggctat tgccgaaggc atagtcgaac agggcaaagg cattctccct ctggatatga
     4321 taaactcctt tacagaagag ttgtcaaaac aaggcgaagc actcctccgt accgggcagc
     4381 agctgctgga agaggccgga aaaaccggca aagaactgct ggatgttccg caggatattg
     4441 ggaaaaaagc ggctgaaggt cttaaaggac tgctgcccgg caagaaagag gagtaatgct
     4501 gttggctgtg acggcggtta tcctgctggt cctgtgtttt actgaacggc tatgcaaaat
     4561 caagaacaga taagaaacgg ggtcattctg catccgggcg ctgtcgggga ctgtcttctg
     4621 acacttcctc tggctgatta tatgaaaaaa gtgttcggat tagatcagat ggattttatc
     4681 ggaagaaccg aatatattga tttctatccg ggccgaacct gtattgaccg cattcgttcg
     4741 atcgaaagca ttgaactgca ccgtttattt gaagataaaa atagtttttt tctcgaagat
     4801 aaagaccgcc ttttagaggc atttgccggc tatgaacaaa tcgtcagttt tctcggcgcc
     4861 ggtcatcctt cgttcgagac aaacctgctt tttaccgttc atagcactca ctcggcagat
     4921 gtggctgttt tatcttcagc aattcccgat gactacacag ctcatattac agacttttat
     4981 ttgcagcagt tcctaaatga acatcatctt gaacaagcag atgccaaaat agaatccgat
     5041 gcattgattg tgcctgttgc ctccgattat tctgcggggc aggagatgct cgaacagatt
     5101 ggaattcagc atgaacagcc gattgctgtt attcatcccg gcagcggatc tcctgctaaa
     5161 agctggcatt ggacgaatta tttgcagctt ggttcagaat taaaggcaaa taatatccag
     5221 actgtcttta tcttgggacc cgccgaacaa gaccgaatgc cggaagaatc ggctcaaatt
     5281 cgagaaaagg aaatcgttct ggaaaacctg actctcggac aagttgtgca gctgcttacg
     5341 caagcagatg tttttgtagg aaatgacagc ggtatcagcc atctttccgg gatgcttggc
     5401 aaacgaacaa tcgcgttatt tggaccgacc aatcccgctc atttccaccc gctgggccgt
     5461 tctgtgacaa ttctcactcc caatgcagac agtttctctg cattcaattt ggcagaatgc
     5521 aaaaaggttt tcgattccgt cattcaaatg ttgtgattga ttccttcaat tcaagatttg
     5581 acgcagcaaa tcgcaatggt ataatgacct ctcgtaatat gaacaggact gattatgtct
     5641 ccaaaaacta tacaggaaat tgctgaacat gttggcggcc gtgtgattgg ccaaggtgat
     5701 gtccttattt catcggctgc caccttggat aatgccggac ccggacagat aaccttttta
     5761 agcaatccca aatatacctc gcgtgtaaaa acaacgcagg ccgcagggat tcttgtttcc
     5821 aaacaggttg aaactgacgc cagtcaaatc cttgttgaag atccctatta tgctttcatg
     5881 caggctgttg tcttgctgca tggtcaccgg cgtcatccgc agaccggcat cagcgagcag
     5941 gcctccattg cttcctcagc ccatatcggc agcaactgca atattcatgc ctttgctgtc
     6001 atctgcgaga acgccgttat tggaaacaac tgtcagattt attccggcgt gtttataggc
     6061 cccaacgccg taatcgggaa tgactgtgtt ttatatccca acgctgtcat ttacgatgga
     6121 tgccggctgg gaaatcgggt gattgtgcag tcgaatgcat caatcggaca agacggcttt
     6181 ggttttgcaa cacacaaagg ccaacatcac aaaattcccc agattgggat tgttattctg
     6241 gaagacgatg tggaaatcgg ctccggctgt gttgtcgaac ggggcacact ggatgatacg
     6301 gtaataggaa aaggcagcaa actgggcgac ttggtagcca ttggacacgg cacaaaaatc
     6361 gggccgcatt gtcttttagt accgcaggca ggtgtggccg gctcggctga gctgggacat
     6421 cactgcgttt tgggcggaca agttggtgtg gtcggccata tcaaagtagg caatatggtc
     6481 aaaatcggcg ccaaggcagg agtcattaat gatgtgccgg atggagccac acttgtcggc
     6541 actccggcaa ttgatgcgaa tacagccaaa cgcgcatatg cactgatcga aacgctgcct
     6601 caaatgcggc agaagctgcg tcagctggaa aaacagatcg agggattaac cggcggtccg
     6661 aaatcctaag ccagatggaa acaaaaaaaa tactgggtct gatagccggg caagggcgtc
     6721 tgccttttct ggtggccgat ggcgcccgaa aggcaggtct aacagtgatc tgtgtcggac
     6781 tggctgacaa tgccgagccc cagctggcgg aacacgcaga tatattcttt tacggcagca
     6841 ttgcccgccc cggcggatgg atccgcaagc ttcgcaaata cggcgttcgc aatgctatta
     6901 tggtcggccg ggtttccaag aaacagattt atacgccttg gaggatagtc aagtatctgc
     6961 cggattggcg ggccctgcgg atctggtact ggcgtttgcg gaaaaaagac aaacgaaacc
     7021 atacggtgct atgcgccata gccgatgaat ttgccagcgc cggcatcttt ctggaggatt
     7081 cgacaatgta ctgcaaagaa cacctgtccg actgcggcgt catgacagcc tgccagccgc
     7141 ctgcttatgc atgggacgac attgatttcg gatggcctct gctcaaaaaa gtcagcgaac
     7201 tggatatcgg gcaggcgatt gccgtacggg agcgggagat tatcgcggtt gaagccattg
     7261 aaggcacagc ccgcatgatc gaacgcgccg gccagctgtg cagaaaaggc ggatggacat
     7321 tgctgaaagc cccgcgctgc catcatgaca tgcgctttga tgtgccgtgc atcggaccgg
     7381 atacaattga aagtctgaag cagaacaacg cctgctgtgt cgccctcgaa gcaggcaaaa
     7441 cgctcatcct tgacaaaccg caaacactgg cactggccga caagctggga atagccgtcg
     7501 tgggattagc cggcaccgaa cgtggtcatt gattcaagga ggcggccaag aacttccgct
     7561 tccagagaac tttcgttctt gtctatgcgt tttttcagaa tagccgttac ctcatcgccg
     7621 agaactctat acaggcgctc cagacagttt tgagctgctg ttcgaacgga cgaaacaggt
     7681 gcagccagag cttctgcaag gcaatggata acatactcaa ctttcatccc ttttttcaaa
     7741 accaagtcgg gcaattcatg gtctgatacc ccttgggcat ccgcggcaaa tcggactgca
     7801 tcgatatggt caggcagatg aacattggcc atcatgcaat aggtctgaat atagtctctc
     7861 agcagccgca gccagcggtg atccgttgta aaggcattgt acgacaggtc gctgtagcga
     7921 agcttccgcg gctgtccttt cttatccaga taatggagcc ggcgtccgtt gaaataaatc
     7981 tccacggcat ccagcatggc tgtcaggagc gattctccat taaaaccgct gaactttatg
     8041 ccattaggag gagattccgt gtcgccaaaa tctgcatcgc atacggtgcg gatcaatcca
     8101 tcctgatgat gtgcaatgac aggagtgcct cctgcgtggg cctcctgctg gctgattccg
     8161 cacggttccc caacctcgcc gccggagggc attacaaaaa actggcttcc cacatataca
     8221 tttcgataca gggatgccgg cagagtccct tccggataat aagcaaaacg gccccgatat
     8281 tcgggcatag atgcaatccg ccggaaatgc atatcaaatc ggcggccgtc ccaggaatct
     8341 tccaccctgc cgcagataac aaactggatg cggctgtcgc agtttaggaa atattccaac
     8401 accgtcggtc ccatcatttt ccagggttca taggttatcc accgattccc tttatcctga
     8461 taagccttcc agtccaccag cagttcaaaa cccttctgct tgctgatgcg gtgaagcatt
     8521 gagaaaatga catgatgatg cgtatcgtca tccggggtca ggcgaatagc cggatcggtc
     8581 tgcaaggcgc gtttggcaag cagtttggcc cgggtatatt ttgcaaaaag gcgtttgcgg
     8641 gcagcacggt cgtatgtttc aagaatgctt ttcttgcagc tgaaccaaaa cctctgccgg
     8701 gccgccatat taaagccgtt gcggcggccg aaaaaacggc cctccgcttc catctgccgg
     8761 aacaaatcgg catagccgaa aacctgcggt tcatccagaa tccgccgctt catagcacgg
     8821 ctgactgttc caatatgatc ggaggtcttc agcatcaggt aggtcaggtc aatttgctgg
     8881 caaaaaggtc ctgagggatt taagcaaata tcactgtggg cttccaatcc gagaacccgg
     8941 cagtagtaat accggcggaa agtattggct tccgccaaac ggatgctgta tgacggatga
     9001 ggattgtgca tcattccgac aacacgggta ttatcaaaat gcggatcggt gttatagtct
     9061 gtccatcgcc gcaaataagc cccaaccagc cacgtgggcc actcgttcat ctgaataata
     9121 gcagggtaat aattataggc acgaagggct tccagtgctc cctgcgaaag aacccttgcc
     9181 ctccgcagct tgtcttcccc ggtctggccg ccatacggag cgtcaaggta tttgccgttt
     9241 tccagataca atacttcgac acggacatgc ttgccgtgct tgtatcgaag aatctgactg
     9301 cgcaggatat tgacgatatc gggcgaagtg tcagttcgta tggattgaga ggcctcatca
     9361 aattcaggaa aatgaatggc gaagctgtat ccatcgtagc ggggggtata tgttttgaga
     9421 attttatccc gcagcgattc atacagcggc gtcacgacga caatatgaaa tccgagctcg
     9481 cttaacacat cggcaatatc cgtcgtataa atgcccaccc cccctgccgt accgctgtag
     9541 tgggattcag gagaaaccag aacaatcgtt ccaaggttca ttccatgcag gatatcggct
     9601 gccgactggc agacggcggt ttcgaactga ttcccctcgt tgacggcaat ccgcattaaa
     9661 aaatggataa tagcggttcg gcgctgggca ttgtcttcac tgatttccgc cagcagcgag
     9721 gtcaatacat tcaatccgag ttttctcttg tgatcgatcc atgttgccag aagcacgaaa
     9781 tattccgcaa acgcctcaaa gctatggggg gcttcatcgg ctatccccgc tataatccgt
     9841 gcaacatagg tatagcaaac ccgatcctgc tttccgtaaa gcggataccg gacaaacgag
     9901 tccagcatca tctggtgacg gctgacccga ttgcttttat cgatcagctc aaaaacgtgt
     9961 gaagaaacag gtcccaatgt tccgccgaat cccaaatgga tcagctcatt gccccaccag
    10021 aattcttttc gggctcggcg ggaccgtcct tctgtattgt tgtaccgttc ggtaatctca
    10081 taaatgctgt cggggcggat ttgttccagc ggacttcggt caaaagcatt gcaaaggaga
    10141 aaattgaagc gtctgggcac agcctgctga ccctcggcat gggaagcagg aaaattgaag
    10201 acgcacagaa acaaatggtc tttataggca cgcagaaacg cagcaatacg gccgttttgg
    10261 gtattgaggg ggataacctc accgcgaacc agcgccggat actgttccac aaaagcaaaa
    10321 agtccgcgca gatatttgcc gaatccgtac agaataggat aagccgtcag aatccgccag
    10381 cgggccttgt tttcttcatg ccaaggatgg ccaaaccgat tgttaaccca gtgcttccag
    10441 cattccgtat aggcctcatc aaaccgctgc tgcatcgaac cggcggaagc ccctcccaac
    10501 cggctgatat ggtacatcag cggtccctgg ctgcttaata ccaaaaaagc agcagccggc
    10561 catatcatat tctgataatt gctggccggc gactcctcat catggctgtt gaacagcgac
    10621 aacgtctggc cgccataggt cttgtaaata gcattcagat accggagatt atcatttaac
    10681 cccaaggggg catctgccgg ctggccgctg cggatatgtt cgagattctt gatcagcgaa
    10741 ttgtacgggg tggtgcccga tttgatcatc cgtatttcat tgccgtgcca ctgttctgcg
    10801 taaataactg tcgcgtgagg aactgctttc tgtatccgat aacacatata cagcagcggg
    10861 atgttcggct cttcggtttt tcgcaagtcc acctgctcaa atccttttac tgttacctgc
    10921 cccagcagac gggcagtccc ctcaagctgt tcagagatcg gaaaaaccgt atcaaatccg
    10981 tggcctacat cgatccgcag tccgaagcct aactgggcca gctcaatgcc gatttcggcc
    11041 agcgcatcga cattttcaaa atccctcata ttgaccataa agctgtccag ccattcgctc
    11101 cattccccat cccagccggc tcggcgtacc aacttgccgt ttgaatcggc aatatagacc
    11161 ggcatatgat aggcaaaaga gacattggcc cgcgaaaagt gcgacagaac acacagcaga
    11221 accttcatcc ccagctggtc tgctttttga cgcagataga gcagggcttc gatgcccccc
    11281 aagtcgcgcc ggactgtctt tccgtcccag acggaaaaca cagacggatc aggccctgcc
    11341 tcgccggccc agcccagttc cagcgccccc agcggataca gccgtgtata cccttcggct
    11401 cgaatcgctt ccagctgttc ggcaatatca taaaaggtgc cgtatacata cattccatct
    11461 tcatttttta ccggctgata cttgtcatcc agctgcagat taaacgtatc cgcataaaag
    11521 ctgagaaccc gctggccgcg ttcatcgggt acaaatttga tcaggccgtg cgaattttca
    11581 tccagctcct cccactgcca cgatgcgcca ttgtcatcgg aaatctgcac ggcatagtga
    11641 agccaaccgg tccggacagg gaaaacggct gaaaatgcga tcatggaact gccttcagca
    11701 gcatcgtcct gctccattaa aataggattt ctccgccggc cgttagcata ccacagaatt
    11761 cgggctttat atattttcct gcccgtttgg cgggctttcg gcaggcaaat acgaacggat
    11821 gccggctcgg ttcggaagac agtgcggttg attcggctgg gaggaaaatg aaacggacgc
    11881 tctgttagaa tatccgccaa cagcccgctg acttcaagac aaatggaacg gcacacccct
    11941 ttgtcggggt gattccagta atacagtgct gcgtacaaca gacgaagagc ctgatgggcg
    12001 ttatctttgc ggcggatttt ttttgccagt tgggaaatgg ctgtcctgac tttctgcaaa
    12061 tcatccgaaa gaagcatctc ctccagcact gccaaagatg catcgctgga ataggcctga
    12121 cgctcctgca gccatccgcg aatccaccgg ccgtgatcat ggaagatatc cgttctgact
    12181 tttttgaaaa caatagatgc gcaggcgaag gaggcggcca tcgcatctat tttgctcgaa
    12241 tcgcatatgc gaaccgcctt cagcagcttc ggattcaatt catacacaaa aaccactgaa
    12301 ttgctgtctg tataaaaccg cttgaatccg gtcagaatct tttcttgggt cgccttgatc
    12361 agaagatccg attgacaggt gcggacacag ctttgaagga tttgcctgag tttctgacgc
    12421 gccgaggtct ggggcaagcg cagagcttcc tgcaggagca gctgcgtctg aggactttta
    12481 gccaaccggg tccaaaaacg gtccagcagc ataatctcct caatctgcat aagcgtttcc
    12541 agccagtctg gctcatcgaa aagcggcgcg ttttcctgca tttcctgaat cgtcgatttg
    12601 agaatatccc ggtgggcatc ataaaaccgc agagtgtgca tgcgcacctg cgtgatatgc
    12661 agttcaggat tgcaccagtg gtaaagtgct ctgtaaagaa tgcccatcag catccgatgc
    12721 cgatgcggca ggcgtcgttt ttcgacggtc aacaaagcag cattcatcag gatatcatgc
    12781 ggaagcaggg aagccgtgcc ggcggcagaa tctgtaatcc gcacctgcac cctcttgttg
    12841 atcagcatgc gatacaaagt cttggattcc tgcttaatga gggacagtat ttcctttaag
    12901 gcggtagtca ggataagcag ctgatcagaa ggaagttttt gaagggatgt ttcagagcag
    12961 gtgaatttca cgctgtcgga agcatcatag tgaaatacaa tcagtgcatc catacatctt
    13021 ccaggcgtta ttaaaccgtt tcctatctat gccgcgtgct gaaacacttt ttagtctgct
    13081 gtgtccgatt gtaaactgtt agcagcagaa aaacaagagt tttttagttt ttcgatagag
    13141 attgtccttc aaaggcaatt attgcaggca aggcggaaca agattctatt catgacgtag
    13201 agattcgata ggatccatat tggccgcctg ataagcagga tacaatccaa aggttaatcc
    13261 aatccccgcc gatattccaa aggacagcag cagggcttgc agcgtaatca ccgtaggcat
    13321 attgctgaag atggttacaa gaataggaat gcacgtgcct acgaacattc ccagcagtcc
    13381 gccgataagg gtcaaaagga tggtttccga cagaaactgg atcacaatat ctttcttgcg
    13441 ggcgccgaga gcacggcgta tgccgatttc gcgcgttcgc tcactgacgg tcgccagcat
    13501 aatattcata atcccgattc cgccaaccag cagcgaaatg gccgcaatag agcccagcac
    13561 aatgctgaag atccgcttgg tgcgagcggc ctcccggaga agttcaagcg gaacaataat
    13621 ttgataatct tttttcttgt ggtactgctc cagcagagaa gccagtacat cccgggtagg
    13681 aagaacacgc tccatcgaag gcactttaat ggtaatttcg cttaattcga tccgctcccc
    13741 gctggctccg cttgtaccgc cgaattgaat ttgcgtctcg ccgaagcggc ttttcatcgt
    13801 tgccagcggg atatagacat agccgctgac tccgccgccc tgcgaggtgc gactgacagc
    13861 agccggctct tctgcttttg cggaggtatc cttgacaatc ccgacaacgg tgtagtattc
    13921 accggcaatc ttcagctcct gcccaatggg atcatcgtag cgaaacagca gttcctgaac
    13981 cgtttcatca ataacgcaga cggcctgact gtagtgcata tcaataggcg tcagaaagcg
    14041 ccctctgcgg acttcgatcg gggctaattc aggataccaa gccaccgtgc cgacagcctc
    14101 tgcagtaata cggcggttca gatactgcgt ctgctgcggc agccgccgga caggcactac
    14161 cacttgggca tccggaatgg tatcccgaaa ccgctctgcg tcatcatacc gcaggccgta
    14221 ttcccgaatt gtctgctggc ttgcatcggc cacttcgata tccgggggct taaccgtttt
    14281 gagtataatg ttggtgctgc ccagacggga aatctgctcc tgcgcttccc ggcttgcgcc
    14341 ttccccaatg gccagcatcg cgataacact ggctacaccg aatacaatac ccagcgtcgt
    14401 cagaccagac cgcaggcggt gcatccacag acttcgcaaa ctgatttgca gcagagccag
    14461 aatttccgaa atcatccgcc cccctttcca tttcggactt cccgttcaat ccgtccgtcg
    14521 cgcagatgaa tcgcacgatg cgtgcgtgct gcaacatact catcgtgggt taccacaatg
    14581 atcgtcttgc cctgagcatt caattcatcc agcataacca gaatttccct gccgctctgg
    14641 gtgtccagat ttccggtcgg ttcgtcggcc agaataatca gaggatcatt ggccagtgcc
    14701 cgcgcaatgg ctacgcgctg ctgctggccg ccgctgagct cataaggccg atgcttgagg
    14761 cgttcgccca gtccgactcg ctgggccaat gcgatggccc gctgccggct ttcggatttg
    14821 gggatgcgct gataatacat cgccacttca atgttttcga taatgttcag ctgccggatc
    14881 agattgaaag actgaaaaat aaatccaatc tgcttgccgc ggacttggga cagctggtta
    14941 tcatccatca tcgacacatc atgactgccc agcagataac gcccgctggt gggcttatcc
    15001 aagcagccca gaatattcag cagggtcgat ttgcctgaac cgctcggccc cataatggca
    15061 acatactctc cctgacaaaa tgtaatatcc aatcccctca ggaccggcag ttccaatgcc
    15121 ccgatctggt aggacttatg gagattctca atttgtacaa ccggctggcc cttggggggg
    15181 ctgtcaggca cattctggcg aatcatggat tctgttctcc caatccgccg ggctggcgcc
    15241 gtccctgtct catcatctca ctggcagccg cttccctctc ctgagcatcg acaaagccgt
    15301 cctgatttgt atccagccgc tggagaaaca tccgtgcccg ttcaggcgct tcatcggcca
    15361 gactgatttt cccgtcgccg ttggtgtcca tctccataaa ccggtccgcc ggattgcctg
    15421 ccctttctcc gccctctcgg cgcatttgcc ggcggccgga cggcatttcc tctgaaccgc
    15481 ttctgtccgg catgaattca cgccgctgct gcgggtcagc tgcggtcgcc gccggctggg
    15541 actgcgaaga gtccgccggc tgctcagaga cagctgcaac aggctgcatc tcatctgatt
    15601 tggccgcttc agccaacagt ctcggcggat tcaagagaac tcgctggccc tcctgaaggc
    15661 catccaccac ttcaacaaaa cggtcattaa acgcaccggt ctgaatgggc cgttccttgg
    15721 gctggccgcc gtccatcaca taacagactt ttcgcccccc gcgtgtggca acgcactgga
    15781 ccggaatgct gattacattt tccagctggg caataataat ctcaactcgc gcgctcattc
    15841 ccggctttat ctgatcgctg acattttcaa tgctgacaac cgtcgaatag actttcagat
    15901 ccggattgcc gaaaaacgcc tgaggatcgg gcagcggggc tattttctgc acctttccgg
    15961 taaatacctt gtcgggctgg gcatccacga caatgcgcac cggctgtccg acacgcactt
    16021 tgtccacatt cgtttcatgg accttcactt ccacatcttt ttcagcagcg ttggttaccg
    16081 tcataatcag ttcgcgctcg cgaacggcct gcccaacctc gatatatgtc cgcgttccgc
    16141 cgtatcgtcc gcctctgtca ctggatgcat agataaccag ccccggagcc gttgcccgaa
    16201 ccgtgcagac ttcaagctgc cggcggatac gctccagacg ctccttattc agcagataag
    16261 tggcttctga actgttcagc cgtgcctcgg cctgcgccag ccgggatctt gcctgagcat
    16321 aaatgcgttc aagctgccgc tcggcctcca gataatcact gaacaatttc tgtgcatcct
    16381 tggggaattc atactgctca aacagatcca gagatgtctg cgcctgttcc caggagactt
    16441 ttaaacgctg cactttaagc cggtctgcat ccaaatccga acgcgacaca taggatttct
    16501 caaaaagctt ctcggtccag cccaaatctg tgttggcacg tctgtactcc tcttcggcca
    16561 gacgaatatc cgctgtgagc ttccgataat tctgaagcga ctggccgccc agctgattgg
    16621 gatcccggat taagtcggta atgacaggca tttcccccgt cgctttgtac tgctcaataa
    16681 gccggttggc catctgctct ccaagatatt tctgcagatc catccgggca aacttgacat
    16741 tcatcagacc ctgctgaatg tcagaatcat tttgattttt ctgaatcgtt aacgcttctt
    16801 tcgcttctgt gtagttggcc tctgctgaat taaacgttat ttcctgctgg ttgacattat
    16861 cccggagctc cgatgaatcc agctcgacca agactcggcc gttctggaca tcctcttctg
    16921 taatataggt gccctcagga accaggctga cgatcgtggt ggaaccttcc acctgtgact
    16981 tgatttccat ggcgttgcgg gctttgatgg taccgctttc ggtcacgctc ataatcagat
    17041 tgccgcgggc ggcggtatag gtgctgagca tagcgctgtc gacggccgaa tctccgccgc
    17101 ggaagcgata ggccgcataa gccgcaagaa caatgccagc aacaactgca attaaaagaa
    17161 ttttttgtct gtttcttcca gtgattccca taatccatcc ggcttaacgt ttaacagttc
    17221 aatatcacga taaaagttca atttggccag catataattc accagcgttg acgtcgtatc
    17281 attctgtgcc gaaagcagag aatcctgtga ttccaagagg tcacgggatt gggcacgtcc
    17341 ggcctgcaga agcatggttg tactgttgac gcgttcttga gccagttcca gactgatttt
    17401 ttggatttcg tagcggcgag cagcctctat cagctggttg taggcattcc gcacatccag
    17461 cttgacctga tcgacggtct gttcatagtc gcgctgggcc tgtaaagcag aaatcagggc
    17521 ctgccggtat tctgctgctt cggccttttt gtcaagcggc aaatccattg tcagcccggc
    17581 gctgtaggtg ccctcatcaa attgaagacg ataccactga tcggtttctg acgaaggaga
    17641 ccggacattg ctgatgccgg cattcaaatc cagccgggca cgcagccggt cagcggcaac
    17701 tttcactttg cggcgggcat cgtctacgct gtccaatgct gtggctatat ccaaacggtt
    17761 ttgaagcgcc aattgaaccg cttcatcaac ggggaaattg ggttcggcta tcttcatcgc
    17821 ctttaaaccg gctaattcat tgggatccag ttcaatgtcg gcatcgaccg gcagggccag
    17881 tgtcagcttg aaattatcga gcgcatactg ataggatcgc tgggcttcgg ccaaacggtc
    17941 tcgggcctgc agcatccgct gctcggtctg atccgcttgc atttgcgcca gcttgcctgc
    18001 ctgtgccttc agggatgctt cttcataagc cgctcgaagg ctggcatagt tgctttgggc
    18061 atttttaacg ctgtcctgcg attgaagggt ttgaagataa ccgcggacaa tcgagacgac
    18121 aaactccttg cggtaccggc tgaacctgcg gatttggtac agaacccgtc tttcggattg
    18181 tgtgagattt tcctgaacaa tctctttccc accgccgcgc atcagaggtt tagagatgct
    18241 ggcgcttaaa accgaaccca gcgaggttcg cgggctgccg gtgagaaact gcatccagtc
    18301 tattgccaga ctgctgccga tctgtgttcc atccgccaga agctgattaa aaccgacccg
    18361 ccctcctgca tccacagatt catccgatgc cgagcgggca tagccgccgt cgattgtgcc
    18421 aaaccactgc cttacataat tatgccgttc cagcgacaac gacaatgcag aattgtacaa
    18481 atcctccttg tttctctggt acgtccggct gcgggcagtg gctatcgcga cggcctgagc
    18541 cagcgacaga cggccggaag gcatccagtt cggatcaaca atgacatcat tggggtccgg
    18601 ctggacatcg gcaatacgat agttggcctt gaccccatgt tcaggacgcc atttttcatc
    18661 gagaatagta tacacttcct cgtctgtctt ggctttatag tcttcagctg ttttacagcc
    18721 gaataagccg ctcaacaaac aaagcagtcc aatccgcaga tacaatcgct tctttctggc
    18781 caaattaaag ccccactgta aaagtaacta tcctattctg tttaattaaa acagagattt
    18841 ttattactga ttctccgtga aagtcattag aaagcaccaa gatacccatc ttgatatggc
    18901 caaaaacgta tataacatta attatattgc actctatctt ggttacatac agaattgatt
    18961 tttttccgat actttcacct aaccattatt gattcatacc aaaaattttc agccagatat
    19021 ccgtttcccg tttggacaaa ccgccccgtt tctgggatgg ttctggcatc ggtttgtttg
    19081 ctgctttttc caattgtcgg agcaaagcta tccagaaagc agttgccgtt acagccgcgg
    19141 ccttacgttt ggtggcagca gcacgaagac ggcggtctga actcacgaca acaagtcttt
    19201 tgggagctgt attgtccaat atcatatctt caataatatc atccgcctcc tttttggaac
    19261 ccgaaaaatg cacttcaaga taacgcattc cctcaaaagc acttttatca gctggaccaa
    19321 tgccgtcgaa tataatagac ccgcgctgac ggatactgct gagaaaatcg gaaatagccc
    19381 ggcacaactg gacatccgaa aaagacgcaa attcttccat tttctgaact gcacgcagaa
    19441 ggttataccc atcaaataaa tagggcacgg cttaatcctc cgccgcaaag cgtatttctc
    19501 cggcgacaat tgtatacagc accttcgtct tcactgtcca gccctgataa ggacaattac
    19561 ggcccttgga gtagaattta gaactgtcta ccgtccactg cgcatccgga tcaattatga
    19621 ctgcatccgc ccggtcgccg acggccagac tgcccttggc gacattcaga atcgaagccg
    19681 gcttgtatgt cagcatagac agcatctgcg gccagccgca tacgcctgta tcaatcagcg
    19741 ctttgcgata cagaggcagg gcgcattcaa ttcccgcaat accgcagggg gccgtgagaa
    19801 attccagttc cttttcactt ttcagatgcg gggcatggtc tgttgccaga gcatcgataa
    19861 cgccgtccgc aaccgcctgc cggattgctt caatatcctt ctgcgtccgc aggggcggat
    19921 ttactttgta gtagggatcg taattgacaa cggcttcttc ggtcagcaaa aggtgatgcg
    19981 gggtaacctc gcatgtgacc ggcaatccct gtgcttttgc ctgccggata atctcaaccg
    20041 aaccggctgt tgaaatatgc tgggcatggt aacgaacgcc ggttttttta accagctgga
    20101 tgtcccgcca gagcatcagt tcttctgcca gcgggtccat tccgggcagt cccaaaacag
    20161 tggcattata gccggcattc atgacgccgt ttctggcaag agaacgatcc tcgcagtgct
    20221 gggcaataaa gcagtcaaac atggaagcat atttcattgc acgaagcatc accgcagtat
    20281 cctgtacacc gctgccgtcg tcggtaaaac ccacagcccc ggcttgtgcc atcagcccca
    20341 tttcggcaag ttccttgccg gctctttcct tggtgatggc gcccataaca taaatatagg
    20401 ttttacgagc ctgccggccc atccgatgga catattctac agccgtcgcc tcatcgatag
    20461 caggggctgt attgggcata cagacaaccg atgtgaaccc gcctgctacg gcggccgccg
    20521 agccggaggc gattgtttct tcttcttcat cgcccggctc acgaaaatgc acatggagat
    20581 caatcaggcc gggggccact attttatctg ttgcatctat cagacggtct gcagtctccg
    20641 tcagattttt ccctatttta acaacctgac cgtcagccaa cagaatatcg gctttgcgat
    20701 ccaccttgtt tttggggtca atcaatcgcc cgttttttat caaaattgtt tctgaaatca
    20761 ttttggccat tatttttcct tctgctcctg agctgctgcc tgattaacca gaaacaacac
    20821 cgccatccgc accgccagcc cgttggctac ctgtcttaga attacgctgt ttgggccgtc
    20881 ggcaacttcg gattcgattt ccacaccgcg gttcatcggg ccgggatgca tcaccagtat
    20941 atccggcttg gcacgcttta accgctccac agtcaaaccg aaaaagtgcg aatactcccg
    21001 cacagacggg aacggatttc cccccagccg ctcaaattgg atccgcagca tattgataac
    21061 atcaaaatct tcaatgacag catccagatt ataagatacc ttgacaggca aatcctgcac
    21121 ccgggccggc atcagtgtgg gagggcctac cagtgtaact tccgctccca gtttggtcat
    21181 cgcataaata ttgctgcgtg ccacacgcga atgggcaatg tcaccgacga ttccgatctt
    21241 taagccgtcc agcgagccgc ggacctgccg aatcgtatag acatccagca gggcttgagt
    21301 cggatgctcg tgataaccat cgccggcatt gacgacgcag gcattgatgc agcgcgtcaa
    21361 cagcttggga gctcctccgg cactatgccg aatcaccaca atatccaccc ccatcgcttc
    21421 cagattccgc gctgtatcaa tcagcgtttc gcccttactg accgaactgg atttttcagt
    21481 aaattcaatg acatcagcac tgaggcggct ggccgccaga gtgaagctgt tgcgcgtgcg
    21541 ggtactgtct tcaaaaaaca tattgacgac aaccttgccc cgcagcggag gagctttttt
    21601 tatcgagcgg gtgctgaact cttcaaagct ctgggcggta tcaagaatga attcaatctc
    21661 ccgacggctc aggtcgctta agccaatcag gtgctttcga gtccactgaa acttctcggt
    21721 tttggtctgt gtatccatca ctcaattatg acccggtctt cattgtctgt ttccgtaaaa
    21781 tgcacacgaa caacctgcgg ctcctgcaca tccgttttga gccctgtata atcggcgcaa
    21841 atggggtact catgatggcc ccgatcgacc agtaccgcca gacgaaccat gcggggtctg
    21901 cctaaatctg tcagagcatc catagcggcc cgtacactcc ggccggtatg cagcacatca
    21961 tcggcaagaa gtatcagctt atctgtaata tcaaaattga tttcggtact gcgaacggtg
    22021 ggctgcactt ctttaggttc attcagatcg tctcgataaa gggtaatatc caatgtcccg
    22081 ttgaggattt gacctgtaaa ataactgctc agcgctttaa ccagccgctt tgccaaaatt
    22141 tccccccggc tgcgtatccc gataactgct atgtctgtat cagctggaat atccgacttg
    22201 atttgtctga caataccctc gattgcctca ttaagctgct tgccatcaag taaagttttc
    22261 atggatttat tgtctttttt tgcttgtatg tgttttttcc tttgcttggt tactaattca
    22321 gccgaagtaa agaatgaata tagcggcagt ttaataacaa agcaaggatt ataaatagtt
    22381 atcccaaaaa atgctgaatc cgttgaaatt cgagcgtatt ctatggtata gtatgaggtg
    22441 ttaccgaaag cgggaaatca aattgaacaa gatattacag atatatcttt accgtttatt
    22501 agctttaacg cgcttcctcg caataacttt ttcggttctt ttttctggat tggggacagg
    22561 cagagcgttg gcgtccgggg aatccaatcc ctctatgctt cctgttgaac cggcagccct
    22621 tgactatgcc cggatttata cggtggtcca gtatgaccct tccctgacag gacgggggat
    22681 acgaatcgcc tatatatgcc ccagtgaaac ttatatcaat aatcgccccc agaatgacta
    22741 ccgcatcaat gtccatcatt cctgtctgta tgatgccgat gtggcgttcg aagatggttc
    22801 cgatgggaag tgggggcttt cttcccatga aactgcctgt gccggcattt tgctgggtgc
    22861 agatgagaag gcctttattc cggactttgg aatgtttcaa taccgcgggg catgccctga
    22921 tgcctccgtt gatgtctatg aattctggcg gtttgcagct ttatacctgt tcggcaaaaa
    22981 accctttgag gccgatattg taacattaag cttaggcaac atttttgaag aatggtggac
    23041 acgcggtctg gaacaggcag cagatgaaaa agaccttctg gttatagccg gaattggaaa
    23101 cggcgccggc gtgcacacgc ctaagccgct gtatccggca gccggagcca atattctcgg
    23161 agtcggcgtt gttgatgcag cagttgacca acacggcact atcagtcttt gggatttttc
    23221 ttctgccaaa gccgttcact cgagtgttgg acctaccgaa gacgggcggt gcaaaccaga
    23281 tattatcgct cccggaacag ccttggtgcc atcccataca gacaataccg gatatgtttt
    23341 ggcttccaac tggtcttccc tttcggctcc gattgtaagc ggtacagcag ctctgctgtt
    23401 acagaaagcc gccatgtttg aacaaaatga catatacaac cagcccggca aggcgttgct
    23461 gctcaaagcc attctgatga attcagccag aaaactgccc ttctggcata aaggccgcgt
    23521 tgaagcggaa gacgatcctg aaacgccgct ggattatata caaggggcgg gggttatcga
    23581 tggattggag gcctatcagc aattaaccgc cgggccgggg agtccgggat ccgtctcgcc
    23641 cgtcggctgg gacaaccgca tgctggacaa cacccataca agctgtgact atatgttcac
    23701 cgctgctgaa cccaatcagt tcataactgc aaccctctgc tggaatcggg tttatcagcc
    23761 gcattacccc tttaaccgcc tgactgagca ggacacagac ctgcgcctcg aattatgggc
    23821 ggtcaatccg aatgagccca atcagccgat tctgctggat tacagcgaca gcatcaatga
    23881 caatatagaa cacatttatg ccgcatgcag cccgaattac acggtttact tccttcgcgt
    23941 ccggtttaat gacctgcaga cgatgcaaaa ccctgttcgc cagcgctttg ctgtggcttg
    24001 gtcagtcggt cccgaccgac aggcaggcaa tccatggtgg tatgacctca acggcgatga
    24061 ccagattgat gcaacagacc aactgattta tgccatgctc gattccggta tgtttacgga
    24121 tgccgatgcc gtatccttgg ggcaggccct caactgcact ccggaacgga tcaatctgtt
    24181 agtcggcggc tgggatgtct ggaagcgcta tttgaccaat tgggaattgg catcagatac
    24241 tccgcccgcc gtcgacgcac aaaacctgcc cagtgatgta ggaattatcc agtagaaatc
    24301 ctatcgcacc ggtgacatcc gccggggttc caaaccttcc ggctggtatc aatttcaact
    24361 gtttttcctc ttcggcagga tccattttgt ccggccaagt cacaattccg ggggcaacag
    24421 cattgaccgt tatgccgggg gccagttctt tagccagcgc cttggcagcg gctatcagcc
    24481 ctgctcgggc ggcactatac ggagcatact ccgcccacgg cttcacgccg ccgatatcga
    24541 ccatatgaat aatggcggca aagggctgga cagcgttttt ccaatccagt ccctcctgtt
    24601 tgagatagcg gacaaacaca ttagagatag aaagcggggc cgccagatta accgccagca
    24661 tccggctaac ctgcagaggc gtcaacgttt ctgccggctg cctttgaaaa accgaggcag
    24721 aatgtacaac aagccgaata ggtcccagcc gccgggcctg ctcgataaga aagtctgcac
    24781 agtccggttc ttccaaatcg cacgcaacag ccagtgcctt ccgcccgtat tgacgtatgg
    24841 tctctgcggc ctgttgagcg gcagccgcat tggttccata atggcacaga caatgaaacc
    24901 ccttccgccc aagaaaaacg gcgatttctc tgcccagttt tccgctggct ccggtgataa
    24961 tggctgctgc attgtcatac tccataattc atatcgtata gcaaatcccc tgaaccggca
    25021 aggcctgtct gaaattgact ccaattttgc ttgcagacgg caaatccgag attataatac
    25081 acccgaactg aggatacttt aaggactttt catggcttta ttaaatgtaa acattgacca
    25141 tattgccacc atccgccaag cccgccggac cgatgagccc gatcctgtat gggcggctgt
    25201 acagtgccag ctggcaggag ccaacgggat tacagtgcat ctccgtcagg acagacggca
    25261 cattaacgac agggatgtgc gtgtcctgaa agaaacagtg gcctgcaagt tcaatctgga
    25321 aatgtgtctg tcggaatcga ttgtcagaat cgctgaggcg gtcattcccg accaagtaac
    25381 tctggtaccc gaaaatcggg ctgaattgac gacagaaggc ggcttggatt gtgcagcggt
    25441 taaacagcgt ctggcagccg ttgtcaagcg gatgcacaaa aaaggcattt tggtcagcac
    25501 atttatcctg ccggatgaac ggcagatact tgccagtgcc gaggctggct gcgatgcggt
    25561 cgagctgcat accggcaggt atgccaatgc ggtcagcgat aaggacattg aaaaacagct
    25621 gaatctgctc cgggatggac gcgaccttgc tgccgctgcg ggcttgattg tccacgcagg
    25681 acacggcttg acataccgca atatccggcc ggtcgctaac atcgaagggc tttgtgaatt
    25741 caacatcggc cacagcattg tcagccgggc ggttttggtt ggcctgcatc aggccgtccg
    25801 tgaaatgaag cagttgattg agaatccttt ttaacaggag tcacgcatgt tcagcggcgc
    25861 attggtcgca attatcaccc cgttttcaga aggccaagtg gatttcgaaa ccttaaccga
    25921 gctggtggaa ttccagctcg aaagcggcat tgacggcatt gttccggtag gcaccaccgg
    25981 cgagtcgccg acactgagct atgacgagca caagcgggtc atcgaacatg tcgttaaaac
    26041 agtcggccga caggttccgg tcattgcagg caccggttcc aacagcacag ccgaagcaat
    26101 cgagctgacc gaatttgccc gcaaggtcgg cgccgacgcc tctctgcagg tggctcccta
    26161 ctacaacaag cccatgcagg agggcttcta tcagcacttt aaggcaattg ccgaggaagt
    26221 ggatcttccg atggtccttt acaatattcc cggccgctgc ggcggaaccg gtctgacagc
    26281 tgaaacggtc gcccgtctgg ccgaggtgga caatattgtt gccatcaaag aggcaaccgg
    26341 ctcgatggat atggccagcg aaattgcgtc gcgctgcgac atcacaatcc tgtcaggcga
    26401 tgattcgctg acgctgccgc tggcagccat cggcgccaaa ggcgtcatca gcgttgtcgc
    26461 caatattgtg cctgccgatg tcaaggccat gaccgatttg attctcgaag gcgactttgt
    26521 ttcggcccga aaatggcacc gcaagctctt tcccttatcc aaaaccatgc tgtcgctggc
    26581 aaccaatccg atcccggtca aggcggcgct ggcgatgctg aatatggcat cggaagaatt
    26641 gcgtctgccg atgacggtgc tggaggaagc caaaaaggcg cagcttcgcc aactgctcaa
    26701 agactacggt ctgctggact aaaagatccg gcaaacggca ggataaaaat cgggcagcag
    26761 aagcaaagaa ggatgttcat tccaagcgcc gcagctgccg gctgtgccag cggctcagca
    26821 caagcaggga aacagccgca cccacaaggc agaacagcat atctttctgc gtatcccaca
    26881 catctccctg cgtgccgaga aatgcctcag cagcagtccc cgataattca gcaacagtcc
    26941 attccagcag ctcatagagg gcgctgatac tcagacaaaa cgccagaaca accggcgcca
    27001 gccatttgct gttcttcagc ggcgaggtgc gtatcaggag ttcacgccca acaatagccg
    27061 gaacaaatcc ctgcgccaga tgcccgatgc ggtcataatg attgcggctc agatcaaaag
    27121 tgctttttat ccaattaaac aaaggcactt gggcataggt ataatgagct cccaccagca
    27181 ggataacggc atgaagcagt atcagggtat aactcaaatg ggtcagagga aagcggcggc
    27241 aggtcggaat cagcaccagc agggccagca caacaggaaa catctccagc acccacgtaa
    27301 acctgtccgc cggcttcact gccgaccaga gaaatacagc ccccaatacc gccagcaaga
    27361 cagctggata cgctgaaggg cttgtcttct catccatccg cggttttcct ttctcgtctt
    27421 aaatgcggca gatccggttc aatatgaaca atcacattaa ccggctgagt cagctggtcc
    27481 cgaagtgcct gctcaagacc ttcggagatt tcgtgggcct ctgcgatgct cagttcggga
    27541 ttgaccagaa tatgcagatc cagaaaaatt tcacgtccaa cgcggcgggt gcgaagctga
    27601 tgccaattca ggatttttct gtctgattcg ataattgttt gaatctgccg gacagtctgc
    27661 ttatccactg cccgctcggc aaactccccc agacaatcac tgatcacttt tccgccgacg
    27721 aaaataatca ttaatccgac aacaatggca gcaagctggt cgccatacgg gtagcccagt
    27781 ttcacagaga ccgcaccgaa caagacggct acagaactaa aggcatccga acggtggtgc
    27841 caggcattgg cgtaaagaat cgaactgtgg gtacgcacag caatgatgcg ggtccattgg
    27901 taaagggctt ctttggtgat taccgacagc agggctatcc agataaccga ggagctgatt
    27961 gtcacgggtt ggctgccgat cgtataatgc attttggcaa tatccataga cgccttataa
    28021 atcatccctg cccctacggc aatcagcacc agcgcgataa cagccgtcgc aaaggtttcc
    28081 tgccgtccgt gtccgaaggg atgctccgag tcggcttctt tggcccccaa atgaatgccg
    28141 accaacacgg caatatccgt cagacaatcc gaaagcgaat ggataccatc ggcgaagagg
    28201 gaaagagaag ccaccgcaat gcccacaacg catttcagca ccgacagcac gatattgatc
    28261 accattcccc agtaggttac agcgcaaatt gccgaattgc cccgcttgag atcggccatt
    28321 ttactttcct atgacttcac cgcagatgcg ttctatctgc ttggcatacc tgtcggcggc
    28381 agccgcttct ttggcctcta taataacgcg cataatcggc tctgtattgc tggtacggat
    28441 atgaatccag ccgtcgggca aatccagccg gcacccgtca cgggtattga tcagcgcatc
    28501 ggcaaaaagg gatttggcct tttcgataac tgcttgggct tggattgtat ccgcgctgta
    28561 tttggtttta tacatgcagt acccgccgac ctctgcagcc agctgggaaa cagatttgcc
    28621 ggcgtccgcc atcagctgca gcaccagcgc catcccgacc aaactgtccc gcacaggccc
    28681 tacccgcaga tcaataatgc cgccgttgcc ttcgccgccg ataacgcagt tgtgttcaat
    28741 catcgcattg gccacatggg cttcgccgac cggtgtccga atgacccggc agccggcggc
    28801 agaggcgatg tcatcgatca ttcgcgacgt ggaaagattg gccgcagccg cccccttctt
    28861 tcgggagaaa acctgtttgg cggccagcgc cagtgtaaat tcctcgccga tgtaaacacc
    28921 cttttcatca acaatagcca aacggtctgc atcggggtct tgcgcaaaac cgatgtcggc
    28981 tttttcggcg gcgacttgcc ggcataaagc ggtcaggttt tcggcggtcg gctccggcgc
    29041 atgcgcaaag cggcctgtgg gttcggcatt catgcagatg acctgacagc ccagcgcctc
    29101 cagcagcatc tgtccggcgg tgccgccggc tccgttgacg ctgtccagta cgaccctgaa
    29161 atgctttgga atgatggact ctttctggac gatggccagt accttttcca gatgaacctg
    29221 atgggtatcc ctgttttggc aaatcgtccc gcagcccagt gcatcggtcc gttcaaactg
    29281 ctgactgcga aagagctgaa tgatttgttc tgctgtcggc ttgggcggtg caatgccgtt
    29341 gcccagcagc agcttgattc cattgtacgg tgccggatta tgactggcgg taatcacaac
    29401 ccccccggcg catcccaaat ggcgcaccat gattcccact cccggcgtcg aacagatgcc
    29461 cagatcgatg acatccgcac ctgctgcggt caatcccgag gctacggccg aaaacagcat
    29521 ctgccccgat gggcgcgaat cccggccgat tgccacagca cacctacccg agctgctgca
    29581 ttgccgtttc agaaaggttc caaacgcgct gccgtagtct gcggccgtat tggggaaaag
    29641 gttgtgccct accaatccgc gcattccgct gatactgata ataagttcct gcgccatagt
    29701 ttagcttcct ctatcctaag gtaacgaccg agtccgttca aggattgtgc tgagattgtt
    29761 cagcagtttc tcaacctgcg cttctttcag cagcagaatg tctttcaggg cctgaccgat
    29821 atgcggcaga tacgtttcta tataactgcg tttgagcagt tcctctttca tccgccgctg
    29881 tcggcgcacg tgaagtccca gcttgcgccc gcattcctga attgccagct tgatttcttt
    29941 gataatctcc ggataatgcg ccacggcctc cttgctttcg gatgtaaacg gcacccacac
    30001 cgaagcaata tgcaccatca gcatcaccgg tccgctgggc aatgcccctt tgctctgatt
    30061 aaggccgtag gcccgccaat tggtctccat caccgctttg taggtggcac aggccgactg
    30121 ctgatacagc agcggcacgc ggttggcaaa ccgcaccagc cgcatcaggg gttctttgcc
    30181 ttcctcctga tcctcatcct tcatctcttt gcatccgtag gccagcgccg cttcgatgat
    30241 gaagggattg ccccgataca ccgccggctt gcgggtgcag ctgacataaa attccgcttc
    30301 tgttacccgc ttgagaccgt caatgagctt ttcctgccca atcggactga tgcagtcggt
    30361 cggcggtgcc atcagcttgg tggcattgat ggcggcgtgc agtttttctg catctttgag
    30421 ggttatcgcc gccggcttca tattgaccga aagcttggct tttttgatga tttctgctgc
    30481 cagacgctcg ctgacccgtg agaattcccg atgcataaaa tcggccagac gtctgcattc
    30541 ggagtcgcgg gccattttgt acaaaatgcc caattcgacg ccgtagggat gtggtttaat
    30601 ctccgccggg atatagggca tttcctccgt ctgacgcgga tactggctga cggtgccgtc
    30661 gggggccctg tatgtaatcg aagcgtgagg attggccatc gctgtctggg cgaggtattc
    30721 atcgacgctt tggcggcctt tctggtaccg cccttccagc acaattgaaa cgctggttcc
    30781 gctgggctgg tcaaactcgc attcttcatc gagaatcact tccgggcggt tttttgaggt
    30841 atcaatctgc acgtggtaat gtttggccgg cttggaaccg ctggtgcggg agataatctg
    30901 gacaggttcg ccggttgtca gcagaccgta cattcctgct gcgctgatgc cgatgccctg
    30961 ctgaccgcgg ctcattttga gcgtgtgaaa cttgcttccg tacagcagcc gtgcaaaaat
    31021 attcggaatc tgtttgggga caatccccgg gccgttatcc ttgaccgtaa cccggaattt
    31081 gttatccgtt ccatccagcg cctcaatatg caccgatatg gccggcagaa tttgtgcctc
    31141 ttcacaggcg tcgagggagt tatcaacggc ctctttaaca gcagtcagca gcgcttttcg
    31201 aggattgtca aatccgagca gatggcggtt cttggcgaaa aattcgctca cgctgatttc
    31261 ccgctgctta gccgccatcg actcggctgt ggcggcttgg gattttcgca cttttttggg
    31321 ggcagccgcc gcagcggatg tgctgccggc cgcattctcg ccggctttgg ctgaagatcc
    31381 ggcagccggc gtttgaagag tgttgagatt ctccaaggca tccgaagatt cggcgttctc
    31441 tgttctttta cggttcgatt tcatccctga cggatgggcc tgcccctgac cggggccgga
    31501 gtcagctgtc ccgaattcaa accgcatttg ggtttgctgt tttgtcttgc gcgaggtcat
    31561 tcctttgatt cccagataat cacagagcgg ttcgactctg cggataccaa agtcgaaccg
    31621 ctcttgttac atcgttccca ttttactgca gtttacatac atacatttca ttgacaaatt
    31681 ccaaaccagc caattcctgt gccgcttggg catcgggggt tttgtcaaga ctgacagcca
    31741 gaatcgcctt gccttcctcc agcttatgcc ccacacccat agtattaatg ttgatgccgt
    31801 gtctgccgag agctgttccc acagccccga tcacacccgg cttgtcttgg ttgaaaataa
    31861 taaccatcgt gccttccggt gttacttcga tgctgaagcc gtcaatttca ataatccgca
    31921 gaagattacc gctaaaaacc gtcccgcgga tggaccgtgt aatcttatcg gttacgacgg
    31981 tcgccgtaaa gctggactcc aaatctttga tttccggatt tttggtgcgg tctatgctga
    32041 cgccgcgttc tttggccagc aagggagcat tcaccatatt tacaacggta tcgaaatgcg
    32101 gctggagaag accgatggtg aaagccgtcg ccgcagcatc taccggcttt tcggcgatgg
    32161 agccgcggaa ttcaatttta gcgctcttca gatgccccgg cgccaacgtg ctgagcatgg
    32221 tgccaatgcg ctgggcaagg tcggcatagc ggctgacaat cggcggcacg gcaccggaag
    32281 tcgccggcgc gttgagggca ttgcgaatcg gtcccccttt aatggcatcg cacaaaatct
    32341 gggcggcttc gacagcaacc tcgatctggg cttcttcggt gctggcaccg agatgcggag
    32401 ttaccaggca ggtcgggcat tcagcaaaac gcttattttc cggcggctct ttggagaaca
    32461 catccagcgc tgcaccggca atcgtaccgg cactgagagc attatacaaa tcatcttcgt
    32521 taatgatgcc gccgcgggca caattgatga tgcggcaggt cggcttcatc attttgagct
    32581 gtttggatgt aataagattc aatgtctgct cattgcgggg aatatgaagc gtgatataat
    32641 ctgcctcccg atatatcttc tcgatattat ctgttatctg cactcctaac tgttctgcct
    32701 ctttgggggc tgcaaaggga tcatagccga tgattttcat attaaaacca agggccattt
    32761 ttaccactgc cattccgatg cgtcccaacc caatgactcc cagcaccttg ttattgagct
    32821 ggttgccggt atagctcttg cggtcccatg tgccggcctt caagctattg caggccggaa
    32881 cgacattgcg gctcagcgcc agcatcaatg ccattgtatg ctctgcggca ctgatcgtgt
    32941 tgccgccggg agtattcata accaaaatgc ccttgcgggt cgccgcaggc acatcgacat
    33001 tgtcgatgcc tacgccggca cgggctatcc cctttaattt gcccggattc tcaaggactt
    33061 tggccgtcac ttgcgtgccg ctgcggataa tcagcccgtc ataatccccg ataatttggg
    33121 ctaattcgtc ttcgctgatg cctgtacgaa ccacaggctc gaaatcgtcc atcgatttga
    33181 ttaaatcgat accctctttt gccagcttgt cggtaatcag aattttgtac atagaaactt
    33241 ctcctaaaat gtttctgttt caaaagcttc ctgaactgaa acacgttcat cggcacattt
    33301 aaatttcagt ttatcacagg gatacaaata acgcagcgtg caatgggctg caccggctgt
    33361 cagacggctt accagattgg cgcgccctcc ggtttccatt tcgacagcca gatcgatcat
    33421 tttgccttcc ggctgtgaag gccacacagc aactcccaga tcggtatcca gaagagcggc
    33481 tcggataatc aggcgcccgc tgcagtaaga acgaatcccc aatgcctcgg cttcaaactt
    33541 gtccacatgg acaatcagga catatccagc cccttgcgag cgggcaatct cctgcggatt
    33601 cagccctgcg gcttgggagg gtgcgggagg attcaaaatg atattgctgc tgttaaatcc
    33661 cgcctttgcc gtcatataca gatggaaggc cgatgccagc tgctggggga aaccgtaatc
    33721 agcgccggcc gaacgcgggc attcaatcca gaccagtatt tttcgtttct cctgttcttt
    33781 cagattatag tccggcgttg tctttttctc aaatgccccg gggctgaaca ataagcccag
    33841 accggatgcg caccccgaca gcacaaaaac attggaaagc agaacaacaa acacgaaaaa
    33901 tcgtgcaata aaacgcaccg aatacttaag gtttagcgtt ttcaagtacc gtccttataa
    33961 gagttacaat ccagccagtc ataataacaa ggatgccagc aataatcagg tttcgtatag
    34021 ttgtggaact gaaaagccgc tccaaaggaa ggaactttat accaaaaaga gcgtaaagta
    34081 aagcaaaaaa cgctgttatt gccgccgtca ggcaaaaaat acatgccgcc ggctgaatct
    34141 taaatgcctc tatcggatgt ccttgtgcaa atgcctgcgc cgcgtgggtc cagccgcagc
    34201 cggggcaagg cagtccatac cgctgcttaa agccgcagat gcccacccat ctgctgaggt
    34261 caacatagcc ttcagcggcc gcccaaagga gtccaaaaac gaccgcggca gcaacaaaaa
    34321 tacccgctgc cagaagtctc attctgccgg acagccgccg attattttct gtaaattgca
    34381 tattggtctt ctgctgttct ttgggtacaa tagacaccca gctacatctt gtcaagacgg
    34441 aaaggatgca tatgtcaata ccccatacgg atctgagtca aatgctggaa acggctgtcg
    34501 tggctgcccg ccttgccgga cagcgggcga tggaagaact gcggtatatc cgcaaattgc
    34561 caaaaagcga taacgagttg gtgacacagg ccgatccggt gtgtcagaaa ataattatcg
    34621 actgcattcg cgaacattat cccgaccatg gttttctttg cgaagaaggc cccaacgggc
    34681 agctgtaccg catccagcct cgcggggagg aggccgtttg gtggattatt gatccgattg
    34741 acggcacgaa taatttcgcc aacgggctgc tgtgtttctg cgtcagcatt gcagcgatgc
    34801 tcgaggggca gccgcttctg ggtgttgtct ttgaccctac aaccgattcg atgtattcgg
    34861 ctgcacagga tatggatgcc ctgctgaacg gttcccggat tgttgtcagt gatgatgcca
    34921 tcgggccctt tgccagcttt gccattgaca gccatacagc accgcaaaca gattctgccg
    34981 tcaagcagat aatgcagtcc acccgcttcc gctgtctggg ctcaaccgcc ctgcatatgg
    35041 cctatgttgc caaaggggct atgataggga tgttcagcag ctctgcccgc ttgtgggata
    35101 ttgctgccgg cgctatcctt gtggaacgtg ccggcggcag ggtttccgat ttgaaaggca
    35161 atcccctgtt tcctgtcaat ccccagcttt acaatatgga acggctgccg acgctcgcag
    35221 cttcggcaaa aatatatcca aaagctcttg aactattcgc aggttaatgc tattttattc
    35281 aaaattagtt gaaacgttac actgcccttt aacgtcttac tgtaaaaaca gcaaatttta
    35341 aaaaggagaa tcaacatgaa taccaagcgt ttatttatag aagtattact ggcatctttc
    35401 attttctttg cttgtctgcc ggcacaggct gccaatggtt ccgacgatgt gctgctgaac
    35461 acgctgccgg ataactgcct gttttgcctg cgggtcaacg ccttcagcac caccctcagc
    35521 aagctggata tgtacttggc cggcacatcc ccaattccga tgagcatcgc tatgctggcc
    35581 aatatgcagc ttatcggcat caccggcgat ccaatgcttg gcggcatcga ccagaacgga
    35641 gattttgcgg ttttcggact gctgaatcct gacgctgccg cgggggttcc ggtcataggg
    35701 ggtgttctga ttcctgtcac caactatgca gattttgtca agaacaatcc aaactgcaaa
    35761 gccggcgaca gcggcgttac ggtgctgtcg gctcccaatt cacagatagg cagcctcatg
    35821 ctgacagaag cagccggcgg gaaatatgct ctggcggtca tggaaggcca gcgggacact
    35881 ctggggacgc tgaaaacagc cctgtcggcc cctgctaaac cgctggctgc aagactccgt
    35941 ccggcgcaca gtcaaacggc cgctgctgct ccggcgtggg gctatatcaa tctgtccctg
    36001 ctgtacaaca cctatcagcc gatggtgctc aatatgctcc agcaggccca gagccaaatc
    36061 ccggcagcca gcgaagagat ggcaggcatg acagcatttg tcctgaagat gtatgccgag
    36121 ctttttaagg agtttgccaa agatgccgac tatgccgctg tgacattaac gccggaaccg
    36181 gctctgctgt gcatcgatac cgccctgcaa accaaagacg gcagccagct ggctggaata
    36241 cttacggctt cgccgcagcc ggccaaaggc ttcaccatgg ctggttatct ggacgatgcc
    36301 agcgccatca acggtctggc aaagctgaat cttgcttcgg tgcagaaaat gtacgacaag
    36361 atatttgata tcctcgcagc tgcatccgag caaaccgcca tgaaagacca aatggagacg
    36421 atgaaggcca tgctgaagaa atcgattgct gcaatgggtg aggaagttgc cttttccttt
    36481 tcctatgcgg gcagcaaacc gccggtcaaa cttcgcgaga ttgtggccgt tcgagacagc
    36541 gccgccatga aggaactgat gagcgaaaat gtcgcgtgtg ccaatctgat ctatcaggcg
    36601 atgggatttc cggccgtttt caaatatcag ccgaatgttt cgacctataa aggcatctcg
    36661 atcgatgcag catccattga gatgaaactg ccgcaggatc ccaataatcc catgaatgcg
    36721 gccatcgaac agatgtatgg cgatgcgttt gcctatcata tggctctaac gcccgacaag
    36781 tttcttatga cgatggggac cgacagtcag gaaacccttc agaaaatgct cgatcagccc
    36841 gccgccgcag caccagccgg cgatttcaaa atggcttggg agatgcttca gaacacccct
    36901 tacagcgaat gcgtatgcag cattaatatt atcaagcttc tcacagggct gggcgaaatg
    36961 atgcaatcgg tggaaccagc ggcatcaacc cccattcccg atattttcgg agggctcaaa
    37021 ggcatgcaga cgcaaagctg tctggcgctg ggcggttttt ctgccgatgg tcaacttgcc
    37081 attcgcgccg ctttccccaa acagcatttg acggaaatca tttccgctgt catgaagatg
    37141 cagcagaaag gaatagaagc ccagcaggcg gcaataaagc ccggcgtcca aactgcagcg
    37201 cctgccgtgc cggaagcccc ggccagtcag aaacggcctg cagccgatga tgcgtctgag
    37261 acagcggcga ttctgaaaag ctggatcggc aagccggcgc cggaactgcg catggttgat
    37321 ctggaaggca aaatctaccg cgtctcgcgc ctgaaaggca aaaaagtgct tctggatttc
    37381 tgggctacat ggtgtccgcc ctgcaaagaa tccattcctt tccttgttga actgcggggc
    37441 aaaaccagtg atgccgatct tgttattttc ggattgtccg atgagccgac agaccggctg
    37501 aatcagtttg tcaaagattt caaaatcaat tatccgatta tttcctatgg ggacaatctg
    37561 ccggcaccgt acagccagat taccgccctg cctacggtgt ttgtaatcga caccaagggc
    37621 attattcgcg atatcatcgt gggatttgaa aaaccgcagg atattctgac ggcgctaaac
    37681 aaaatcagct aagcggccgg atgcctgctt ctgctgccgg ctcctgcacg gggactgctc
    37741 tgtcaggcgg atgctgcaga acagcagggg tgtctgtgtt cagcggggca attggatttt
    37801 gactggctcc tttttaagct cgctgacggt cggcagaatc tgccagcggc agattccgtt
    37861 tgcctcacaa aacagccgga caaggtctgc aatctgctcc ctgcttccgg cgtggcccat
    37921 cgcatagagg ctgaaaggcc agccctcaac ggatcggcgc tggtagcaat gactgaccca
    37981 cgggcacaaa gccaaggcct gccccgccgt gcggatgctg tcttgggcaa cgtgtgcaca
    38041 aaacagggca ttggcggcat accccaaagc cggataattt acgacagcag caattttcct
    38101 gacaacaccc ttttgaagca ggctctgcag cgctgcgaga aaatcggctt ctgttattgt
    38161 gccgtcttgg ccggcaagaa cagcaaaggc atattccgcg acaggcaggt cctgctggat
    38221 cagatgaagc gtatgtcgtt cgctgtccga cagctccgcc ggctgccggg ggatatcgga
    38281 caatgcaatg tcttggaccg gctggggcat cgggtccgat acagatgcaa aaggcccggc
    38341 cggctcaaaa tagacatcga gcttaaacag ccgctgtgcc ggcagactgt gaaattcaac
    38401 ctgcagctgc tcctgcaagc cggccaattc cgcatccacc gctatcaaag acggcgcctg
    38461 catcgtaaac cacaggttat agtagtggtc gcggcagtag ctgtgagaca ccgcaggcag
    38521 gcggctgacg gcatcggcaa cctcgctgaa gcgctcggcc ggcacatgag ccgcaatcag
    38581 cgaggcagtc cggcctaatg cccgatagtc aatctgtgcc cggatacggc ggataaggcg
    38641 ctgctgctga agctgacgga tggttcggat gacctgttct tgcgttgcag acagccgctc
    38701 ggccaaaacg gcaaacggcc ggctgcaaac cggcaacggc cgctgaatcc agcgcagaac
    38761 atccatttca aaatccgtca gtgtcatatt gcttattcta tttccatcgg ctttcgtttg
    38821 caatccgcaa ggcgggggaa ttcatcctgc cggccaattt tggaggttgc agatactact
    38881 gttttgtggt atattttttg gcgaacaaaa cccatcagac ccttgaaagg aaaacttgat
    38941 gaaaaccgtg caaaacacct cgacaaaaac gaaaatgctg gatttgagct taaaatacaa
    39001 agtagcagac ctgtcgctgg ccgagtttgg ccgcaaagaa atgcagctgg ccgaaaagga
    39061 aatgcccggc ctcatggcga cacgggaaaa atacggcaca gaaaagccct taaagggatt
    39121 gaaaatcacc ggttctctgc atatgactat ccagacggcg atgctgattg aaaccctcga
    39181 aattctcggg gctgatgtcc gctgggcctc ctgcaatatt ttctccaccc aagaccatgc
    39241 cgcagccgcc attgcagcgg caggacgcag cgcagtcttt gcttggaaag gggaaacgct
    39301 ggaggattac tggtggtgca ccgagcaggc gctgacatgg ccggacggca gcggaccgga
    39361 tttgattgta gatgacggcg gcgatgccac attgatggtg attcagggaa tgcgggttga
    39421 gaaagacccc tcgctgcttg ccaaaacata cgacaacaaa gaatttcaaa ttattatgga
    39481 ccgcttggcc gccagttatt cagccaatcc ccggcgctgg acaaccgtcg cccaaaacct
    39541 caaaggcgtc tcggaagaaa caacaaccgg cgttcatcgg ctgtatcagc tgcaggagtc
    39601 gggcgaactg ccttttccgg ccatcaatgt caacgattcg gtcaccaaat cgaaatttga
    39661 caatctctac ggctgccggg aatctctggc cgacggcatc aagcgggcaa ccgatgtcat
    39721 ggtggccggc aaagtcgtcg tcgtctgcgg ctatggggat gtcggcaagg gctgtgctca
    39781 atcgatgcgg gcactgggag cccgcgtgct gattacggaa atcgatccga tctgcgcgct
    39841 gcaggcattg atggagggtt ttcaggttac cactatggaa gaggcagccc cgcaaggcga
    39901 catttttatc actgctaccg gctgctgcga tgtcatccgg gccgaacata tggagcagat
    39961 gaaggacgag gccattctgt gcaacatcgg ccactttgac agcgaaatag acatgcatta
    40021 ccttgaacac agcccgctgt gcaaaaaaac caacatcaag ccgcaggtgg acaaatggac
    40081 acttccctcc ggccggtcgc ttatcgtgct ggccgaaggc cgtctggtca acctcggctg
    40141 tgcaaccgga caccccagtt ttgttatgag cagcagtttt accaaccaat gcttggctca
    40201 gattatgctg gctaccgaat cgctggagcc gggagtttac accctgccaa aaaagctgga
    40261 tgaagaagtg gcccggctgc atgtcaaggc gctgggcggc aaattaaccc atctgacgga
    40321 aaaacaggcc ggctatttag gcgtacctgt gaacggtccc tttaagccgg accattaccg
    40381 ctattgacag cagcgtccta cacggcggca agcggcactg acaaatacat tgcggaccca
    40441 agcccaaccg gcggagcggc caaaccgtct tgtctggacg gcctcggaca ggatttctcc
    40501 aaaccctgcc ggcccaacgg ctgttgttgg ccctgtcatt ttggcatact ggacctcctg
    40561 aaaggacaac aagtctttat tatgcaagat gttagagact ttccaacttt cgggcacggc
    40621 atttgctgcc tttgtatgcc ggttccatga cagaaaggac aatcgtatga aatttgataa
    40681 attcacggta aaggcccaag aagccatcgc cacaagccag cagcttgcac tggcaggccg
    40741 tcatacggtg gttacgccgc tgcatctgct cagcgccctg ctggacgatg cggatggaat
    40801 ggccgttgag attttgaaaa acatcggcac caatgtggag cggatccggc agatgaccga
    40861 aagcgagctg aaccgccagc cgaaaggtca ggtcaacggg atgatcatgc ccgatgcggc
    40921 gttcagtcag gtcgttctgg atgcgcaaaa caaagccgac aagatggggg atgaatttct
    40981 cagcatcgag catctgctga tggctctggc cgatgtgaaa agcgatgcca aagaaatcct
    41041 cagcgtcaat tcgatttcgg tcgaggcgat caccaaagcc ctgcaaaacc tgcgcggcga
    41101 tgcgaaagtg accgatgaca atccggaaag caaatacaaa gccctcgagc gctacggcat
    41161 cgatttgatt gcacaggcgc agaagggcaa actggatccg gttatcgggc gggatgacga
    41221 gatccgccgc tgcatgcagg ttcttaaccg ccgcaccaag aacaatccgg ttctcatcgg
    41281 cgagccgggc gtcggcaaaa ccgccattgt ggagggactg gcccagcgga ttgtagccgg
    41341 cgatgtgccc tcggggctga agaacaagaa aatcatcgct ctcgacatgg gggctttaat
    41401 cgccggaacc aagtttcggg gcgaattcga agaccgcttc aaagccgttc tgaaagaagt
    41461 catccagtcg gacggccaga ttatcctctt tattgatgag ctgcatacca ttgtcggcac
    41521 cggcaaaacc gaaggctctg ttgacgccgg caatctgctc aagccgtcgc ttgcccgggg
    41581 cgatctgcgc tgcatcgggg ccacgacgct ggatgaatac cgcaagtaca ttgaaaagga
    41641 tgccgcactg gagcggcggt ttcagccggt aatggtcgat ccgccgacgg tggaagagac
    41701 cattgccatc ctgcgggggc tgaaagaccg ctacgatgcc catcacggcg tgcggattac
    41761 cgatgcggct ctggtggccg cagccgcctt atcgaatcgg tacatttctg atcggtttct
    41821 gccggacaaa gccatcgacc tgatggacga ggcggcatcc cgcctgcgga ttgaaaacga
    41881 ctcgctgccg tccgaactgg acagcattcg ccgggaaata atgcggttgg aaatcgagcg
    41941 ggaagccctg aagaaagaaa ccgacgcggc cagccgaaag cagctggaga aatgtgaaaa
    42001 gcagcttgcc gaactcaaga cacagaatgc gcaactgaca gcccggtggg aaaatgaaaa
    42061 aggagccatt gacaaagtaa aagccctgaa ggaaaagatc accgctgttc agtcccagtt
    42121 tgaggaagcc cagcggcgtg gggatctgga acgcgcggcc cgtctgaaat acgaaacgct
    42181 gctggggctg cagaaagagc tcaaaacagc cgaagagagt gtcgccaaag ccaacggctc
    42241 ggcgatgatt cacaacgagg tcacggaaga gcacatcgcc caagtcgtca gcacatggac
    42301 aggcattccc gtcagccgtc tgatgagcgg ccagcgggag cggctgatga agatggaaga
    42361 ggaaatccgc cggcgcgtcg tggggcagga cgaagcggtt gccgccgttt gcaatgcggt
    42421 ccgccgcagc cgtgccggat taggcgatcc ccatcggcct atcggaacct ttctgttcct
    42481 cggtcctacc ggtgtgggga aaaccgaact gtccaaagcc ctgtcggagt ttatgttcaa
    42541 cgaccccaac agcatggtgc ggattgacat gagcgaattt atggagcagc attcggtcgc
    42601 ccgcctgatt ggagcgccgc ccggctatgt gggctatgaa gaaggcggcc gcctgactga
    42661 ggccgtgcgg cgaaaaccct attcggtggt gctgctggat gaaatcgaga aagcgcatcc
    42721 ggatgtcttt aatgtgctgc tgcaggtctt tgacgacggc cggctgaccg acggcaaggg
    42781 ccgaacggtg gatttcaaga ataccgtgat cattatgaca agcaacttcg ccagccagca
    42841 gattctgcag ctgagcgaac aaaacggcgc cgactgggaa atcgaagccc atgtcaagga
    42901 agccctgcgc aattacttca agccggagtt tctcaaccgc atcgacgaga ttatcatctt
    42961 ccatacgctc aaacgggagc atctggagca gattgtcgat attcagctgg ggtatctgat
    43021 aagccggctc aaagagcggc agtttgccgc agaggtaacc gcagccgcca gaaaactgct
    43081 gatggacgag ggctatgacc cctctttcgg cgcccggccg ctcaagcggg tcattcagca
    43141 gcggattgaa aatgagctgg ccaaagaaat cattgccggc aaatttgccg aaggcgacac
    43201 tatccgcatc gatgcggatg cgcatggatt caaatttgtg aaggtctaac atgctgacgg
    43261 tctggctggt tctgctgatt ctggtcaatg ccggctggct agcaacggtg ttgtttatgc
    43321 tgccgggcaa ctggctgatg gtcgtcagca cctgcctgtt tgcgtggtgg aaatgggaca
    43381 caggcgtatt cagctggccg ctgctggccg gcatcacggt gctggcgctg gcgggggaac
    43441 tgattgaatt ttttgccggt gccggcggcg ccaaaaaagc cggtgccggc tggacgggcg
    43501 ctgcttgggc cgtcgtcggg gctgttgcag gggctgttgc aggaaccgcc gtcattccgg
    43561 tgccgctgtt tggaaccctg ctgggggcct gtctgggggc aggactgctg acttgggcgg
    43621 ccgaacggac agccggcaga ccgaaacagc attcggtccg ctccggcgtt ggagcaggaa
    43681 ccggcgtgtt ggtcggcatt gccgccaaat ttgccgtcgg ctgcctgatt tggctgatga
    43741 ttgccgtggc tgcattctgg ccgtaatgtc cgcttggacc tcattgatgc cctcctgcct
    43801 gaaaagccgg cattttgtat tgtccggctg attttttcat tgcagccggc gtcctgcttt
    43861 tatagtattt ctcttcggtt gaatcggatt cgggaggatt gtatgaaaac tgtcggattg
    43921 gcttgtgcgg tcgtgctgac atcgggggta ctgtgggcgg acccggaaac atacggcgat
    43981 gtctctgtat cggccctgct gcggattgat gaaaccgtta ccctattttg cgatattgcg
    44041 gactttccgc ccgtcatcgg ccaaaatatg ccggtccaaa tcagagggct caaaaccgcc
    44101 gcttccgggc aggacaacct gccgctgctg gcttttctga atacgttttt cttttccggc
    44161 agcgtccagc acacccttgt gctccggaat atccagcgcg gccaacagtt ctgtctggtg
    44221 gcggatattg aagttgacgg gcaggatttg tgccagctgc tggttgaaaa gaaactggct
    44281 cagcggatta ttgaaatccc ctctgccgcc ggcagccggc cggacgccgc cggggccagt
    44341 gcaggactac aaaacccccc ttccgcctca cagcccagcc cagccggcta tatagccacc
    44401 aaaaccagca aggtatttca tcgggccgac tgccctcata tcaaacgtct ggatgcatcc
    44461 aaaaccgttg ttttcccaac ccggcagaat gcagcggcca gcggacgccg tccctgcaaa
    44521 acctgcaatc cgtgattata tttcccagtt gggggaatgc ttgatcagct tgttctgaat
    44581 cagcattcca atgaccgtcg gaataatcag caccagcggc agaaaccaat cgtgctgaaa
    44641 gaagaaggcg ttcagatgct gcgtgggggg attcatcaca tacttctcgt actgatacac
    44701 cagcgtcagc attccggtca tcataatcag actgcccccc agactggtaa acagcatgac
    44761 cgacactttc agcagaataa acgagatcag gccgccggca ataaaaccga caataaatcc
    44821 ggccggcaga tacgtctgcg acaggccgaa ggcaatccac agcccccctg tgatgatgcc
    44881 gccggccagc gctccgagca ccgaaacgca ccatttcatc agcggcatcg aaacagccgc
    44941 aaacaagacc atcccgatga tcccgcccca cagaaccgga ttgtatgcct cggagttgag
    45001 tttattgcca gccagaatgc ctaaaaacag cccaataaat ccaaaactga tcaccaccag
    45061 cacccgaaag atccgccagc cgtacagcgt atagaccaca ccgaccgaaa tcatcagcag
    45121 agcaaaccgc cagtccagcc ggctgatgct gtcccacagt tcctgcttgg cctgcaccac
    45181 gtcaaacaag ccggcagaac ggccggcaga ttcttcagca acggtcgcca acaatgccat
    45241 tggattcatt ttctattgtc ctcagttatc ctgtctgaaa ccggttcagt cctgtctgca
    45301 cagccggcct cctgccggat aatccatgcc ggcctgaacc gaccctgcac agggcgacaa
    45361 tctggtcttt tttgcgtaaa cttaccctat cttctttatt tgcaagctgt tatatatttt
    45421 tggcggcgtc ttttgcagcc gttgtgcaca ggacagggct tttattgtat cggttcaggt
    45481 gcggatttta cataaaatat cttcagcagc ccccgccgct tcaaaaacga agcctgtcga
    45541 aacaatagac agcaaaaggg ggccctgtcg cagaaacaga gcccccttat ctaccgcata
    45601 aggacggtgc cggcaagaac cgttttaggc cgccggcatc atttttttgc cttcgacctg
    45661 ctgcgtaccg cggctggtgg ttatctggcc ggtcagcttg ccgtcctgca attgtccttt
    45721 gaactcgctt tcaaaggtcc gatcgccaaa gcccatcgaa tatttgaagc tgacttggcc
    45781 gttttcaaag gtgattttgc cgacattgac ggcgccgtac atgcccgtca tatccggcag
    45841 cacaatcagc agctgccggc gctggccgaa gtccgatgag acggacaagt cccatttgcc
    45901 gatgagttcg ctgcccaaac ggcggccggt cacttcccgt tctccgcgct ggcttttgga
    45961 tacgcccgag agagtatcgc cggaagccgt cagttcatag gttgtttcca tctgccggtc
    46021 ctgaaagctg agggagcggg taaatgtgag tttgccgttt tcaaatttca catcggaaat
    46081 cttgctttcc gcctgcatct gggaattggc cgggcccgtc cagtcggccg tcagggtgcc
    46141 gtcggcggct tggctgactg tcagaagcgt gatgatttcc tgttcgttcc gctggcgttt
    46201 gaattcccac acgcccaaaa ccggcggcag cgcatcgagg cgccgccctt catacacaaa
    46261 atcgccgcgg tcgctggagg ccgtcagcga catcttgccc tctttgatgg ttccggaata
    46321 gtccactgta aaaccttcgg actggtcctg cccgccgccg gggggacgga accgaatcgt
    46381 ctgggaaaag ctgattttgc cgtcagccgc tttcagattg tccaccgcat ccgtcccgaa
    46441 aaagctgccc cacaggccgg tcagcttgcc gtctttgtcc ttggcgataa tcaaaatcgc
    46501 attcatcgga cgtccgtcaa agtcggcttt cagcagccag tccccgaccg ccgggtcagc
    46561 ctgctcggcg gccatcagcg gcattgacag ggccgccgcc acggcagcag cagccatcca
    46621 tttcaggtgt ttcattttat aatcctccat aaaacgattg tttttgcatc cggcggcgcc
    46681 gccggaatct gacagaacgc tattatacct tgatttcggg gattatccat ccatcccgat
    46741 aattgttttt cagatacccg ttggccgtcg gggcaagagg accggtaaac tgctcctgcc
    46801 ggccgtcaaa ggtcaatgcc gccccaagga ccgcctgcgt ctgtttcaaa tcgacgccgt
    46861 tggcttccag atgctcggca aaccgctgcc aagcatcggc cagaagggca tcctgtttga
    46921 cctgctgggc aatggcttcc ggcggcgctt gtcggcccag ccggtgggaa atgttggcca
    46981 gatggcagag ggccgaggaa atatggccgt cggcaataga acagggcacg gcatccgcct
    47041 gtccgctgcg gacggcatcg ataaaccgcg tcaaatgcag ttcccccccg ccgctggaaa
    47101 agcggtggat ccgccggccg tcgttgtcat agacggtttc ttcggcaatg tagccgcctt
    47161 cgcattcaat cacattgccg atgcgggttt tcttgtagat cgacatcccg cgaatgcccg
    47221 gctgggcagg cagcccgcgg acctcgaaaa tcagcgggac aggcttataa tcatagaaca
    47281 ggatttgcgt attgggcgtt tgtccggcat cgctgtaaac aaatcgcccg ccgatgctga
    47341 gaatatggtc gggatagccc gtttgcccga gcatccagcg gccgatatcg acctgatgaa
    47401 cgccctgatt gcccaaatcc ccgtcgccgg tattccagac ccagtgccaa tcgtaatgga
    47461 actggggccg ccaaatcggc tcatcctgcg ccggtcccag ccacatctgg taatcgcagg
    47521 ttgccggcgg agcagcggga gtatccagct tgccgatatc gccgcgctct ttatagcaca
    47581 gtccccgcac gcacaggacc ttgcccaaat gcccctgctt gaggtactcc atcgcctcct
    47641 gccagcactg ccggctgcgg tgatgcaggc cggcctgtac gaccagacgg ctcttgccgg
    47701 ccaagttgac cagctgacgg ccttgccaga catcgtgcga aaccggcttt tcaacataca
    47761 catgcttgcc cgcctgcaag gtatgaatgc tgtgaagggc atgccagtgg ttgcaggaaa
    47821 caatcacaac ggcatcgatg tccttgcggt ccagaagctt gcggtaatct gtgtatatct
    47881 ctacttccag cggattatcc tcgctgtggg ccagttcatc cttgagctgt cccattttat
    47941 accccgggtc cgggtcggcc agcgctgcaa gctgcacgcc gggcattttg gcaagccgct
    48001 gggcatgata cgaaccctga cggccgacgc cgataatccc cacccgaatc acatcattgg
    48061 cccccagaat acggctgccg gaagcggcag ataaacccaa cgcaacggct gctgaacctt
    48121 tcaaaaactc acggcgcctc atattcatcg cctaaatccc ctttataaaa gtacttatga
    48181 ttgcattgcc ggcggtttgc cttttaataa agaacgcatc cggcggcgtt ttattgcata
    48241 ccctaagagg ccaacctgtt ttataacatc tgccggggcg ttttgcacag aaaaactttt
    48301 ctgttcgacg gattccctgc ccggcaatac ggatgccgca ataagccggc cggcagccct
    48361 tcaaaatgcg taattttaag aaagcccaaa catcgctctt tcttcttgcc ggccaatccg
    48421 gtaagatagc cggcaggtat tcaagagaca aaaaaaccgt tttgaaggga tttgctacct
    48481 gcatgcgact gatggaactg attgaactgg aacccaaaaa cgatgccgca ccgtcggccg
    48541 gcaatggccg caatgccgaa tacagtctgc cggaggaaat cggcattctg ccgatccgca
    48601 gtgcggtggc ttatccgggc accgtgatgc cgctggctgt cgggcgtgaa cggagcaagc
    48661 ggctgattac cgatattgcg ccgcaggaaa cgattttcgg cttgctgacg caaaaaaatc
    48721 cggacattga agaccctgcg ccgacggacc tttacaccgt cgggaccgct gcgtcggtct
    48781 taaaggtgat tcgcatgccg cagggatcgg tcaatattgt cgttcacgga atgatgcgct
    48841 tcaaagtgct ccgctttacc gccacggagc cttacctgaa agcccgcgtt gagccgctgc
    48901 cggtgcaggt caaaaacacc aagaagctgc aggcgctgat ggtcagcgtc cgcaatacgg
    48961 ccaatcgggt cattgcgctg agtccgaatg tgccggagga agcggctctg ctgctggaga
    49021 atattgccga tccgtcggcg ctggccgatt ttttagccgc aaccctgaat atctccattg
    49081 ccgaaaagca ggccctgctg gaagaaaccg atgcctacaa gcgtctggaa aagatttcac
    49141 tggcgctggc cagccagctg gaagttctgg agctgagcca caaaatacag gggcaggtgc
    49201 gggaatccat cgacaaaagc cagcgtgaat acttcctgca ggaggagatg aaagccattc
    49261 aggccgaatt agggcaggat gaccggcgga cacaggaact caaggagctt caggaaaaaa
    49321 tcgaggccgc ccacatgccg ccggcggtag aggccgaggc ccggcgggag ctggaccgtc
    49381 tgggcaaaac cccgcccggc tcgcccgact acggggtcat tcggacctat ctggactgga
    49441 tgtgcgaact gccgtgggcg gtgcaaaccg acgaccgcat cagcatcgca cgggcccggc
    49501 ggattctcaa tcaggaccat tacggtctgg acaaggtgaa acggcggata ctcgaatttc
    49561 tggccgtccg caagttaaat ccccgcggca aagccgctat tctgtgcttt gtaggccctc
    49621 cgggagtggg caaaacctcg ctgggcaaat cgatagcccg ttcgatggga cgcaagtttg
    49681 tgcggatctc gttaggcgga atacgcgacg aagccgacat tcgcggccat cggcggacgt
    49741 acatcggcgc cctgcccgga cgcattttgc aggaattgcg aaaatgcggc tcaaaaaatc
    49801 ctgtttttat gctggatgaa ctggacaaaa tcggacagga ttttcgcggc gacccggctt
    49861 cggcgctgct ggaggtactg gacccggaac agaacaatac ctttacagac cactaccttg
    49921 acctgccttt tgacctgtcc aatgtgatgt tcatcggcac ggccaattat accgaaccca
    49981 ttccgccggc actgatggac cggatggaaa tcctgcagct agccggctat accaaaaatg
    50041 aaaaactgca cattgccaaa aaatacctta tcgccagaca gctcaaagag cacggattaa
    50101 cgccctcgca gctgaccatc agcgatgagg cgctaggggt gatgattgac tcctataccc
    50161 gcgaggccgg cgtgcggaat ctggaacgca ccatcggctc gatctgccgc tatgtggcca
    50221 cgcaaatcgc cgagggcaag cgaaaacagg ctgccatccg cacccaagac cttctgacga
    50281 ttttgggacc gccccagttt gaaccggaag tagccctgcg gaccgccgtt gcgggagttg
    50341 ccacagggct ggcttatacg cctgccggcg gccaaatcct gtttgtcgag tcggtgaaaa
    50401 tgcccggcaa ggggcaattg cagctgaccg gccagatcgg cgaggtgatg aaggaaagcg
    50461 ctcaggcggc cttttcggtt cttcgctcgc atgccgaggc gctggctatc gagccgaagg
    50521 tcttttccaa atacgacttt catatccatg ttccggccgg cgccgtgccc aaagacggcc
    50581 ccagtgccgg cgttgccatc tttacctcgc tggcgtcgct gctgaaaggc attcccgtcc
    50641 gtccggatgt ggctatgacc ggcgaaatta ccctgcaggg gctcctgctg ccgattgggg
    50701 ggctcaaaga aaagattctg gccgccaagc aggccggaat caagacggtc atcctgcccc
    50761 agcggaacaa aaaagatatg ctcgaaatcc cccgcgaggc caaaagcggc atgcagtttg
    50821 tctatgcccg cagcgccgct gaggtgctca aaacggcctt ggctgccgtc gtcccgcagg
    50881 ggggcggcca agtccccgcc agacggcgcc aaaaaaaact gcagggcaaa aaaactgctt
    50941 tgccgtcaaa aaaatagttg acagaagccg tcggccgctg tttaatgcgg actttgcgtt
    51001 cggggccgta gctcaattgg ttagagcatc ggactgtcga tccgaaggtt gagggttcga
    51061 gccccttcgg cctcgtttgc tgccggcctg ccgctaaaat cccaccgttt gcggcgggct
    51121 tggcagtacg cattttactt tttctccctc ttaacaggga aatttctgcg tcggctcaga
    51181 ggagccaaac cggccggaga tttcctccat ccggccgccg gcaagagaga tttgtcgctg
    51241 cctgtgccgt ctgagacctg tgaatacctg cgcctatgga
//
LOCUS       DAOTBO010000010        50750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_560_length_50750_cov_5.832015, whole genome shotgun sequence.
ACCESSION   DAOTBO010000010 DAOTBO010000000
VERSION     DAOTBO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 50750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50750
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_50750_cov_5.832015"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLE88_02225"
     CDS             <1..797
                     /locus_tag="PLE88_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOM75313.1"
                     /translation="AKRTLPVNCIRPNPYQPRTSWDEEQLKGLCESIRRNGIIQPIIV
                     RKNTEGYEIIAGERRFRAACMAGLQEVPVLERAATDDEMLELALVENIHRSDLNPIER
                     AKAYQSFINTFNLTQTEAAQRLGEDRTVIANYLRLLDLPTDIKQMLSGGQLTMGHARA
                     ILSLPSDDLKRKLANRAMAGRLSVREVERLVRIYIEKKDEKLLKKEKSPHIADLERRL
                     QEVLGTKVNIKPNKRGHRGRIIIDYYSIDEFERLTEKMGLTFEDGD"
     gene            881..1588
                     /locus_tag="PLE88_02230"
     CDS             881..1588
                     /locus_tag="PLE88_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75314.1"
                     /translation="MVKKSPFEAVYRQLGARFDIFYDWLMPADFGDIEAENNAIKNHC
                     AAVDLSCFGRISVKGGSNKEVLSSIFKYRSGSLALDRWIWAKTTKNGKDLLCRIVRTN
                     GDCIVITSPGQNEMVMELLTVEGGEAISVSDLTEKTGMLGLYGPAAAKSVRGLLPFEF
                     DSLEIGDAAQLTFFMMSFTLLRGSWLGQDGLELVCPASAGAMAAGAIVKYRNKHNITP
                     AGMESLSRIFADVKPPV"
     gene            1723..2499
                     /locus_tag="PLE88_02235"
     CDS             1723..2499
                     /locus_tag="PLE88_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOM75315.1"
                     /translation="MFAIISDIHSNLEALTTVLEDIEKRGIKTIYCLGDVVGYAANPL
                     ECLDLVIAKTENCVMGNHDYAAMFEPTNFNSGAEAASYWTRKVLEDETDRQKSDRRWR
                     YLGNQKMRWTLETRLGDVDATLDFVHASPRRPINEYVFPDDVYTTTGKMSALFERVKH
                     ICFVGHTHLPGVFLEDPDFYSPDELGGTYPIIPDEKAIINVGSVGQPRDRDNRASYVY
                     VQDNIVNFVRLEYDYKTAAEKIYAIPELDNFEGDRLADGR"
     gene            2576..4657
                     /locus_tag="PLE88_02240"
     CDS             2576..4657
                     /locus_tag="PLE88_02240"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOM75316.1"
                     /translation="MKAKIAIISCAFFFGLCVWLVVDAVKHHPQQAKVLLSRVVMLTG
                     MVEQASESASSDDVSLAQNKNEPTVYPQVSAVSGREQTAVLGALYEDIRRTENPDKYK
                     FQITLTTRGAAVKNAVLSEFKARKKAESAELLTDQADCIKPADNPLILLSEMAHERRV
                     YSLANTELKLAPAEAENFGTKAFPLDKLNWRLAKEPSADQACFEAVLNDAQTGEPLLR
                     ITKTYRVQPGSYDLDCRISIENLSSKPLKALMQMQGPAGIGKEEERQDMRRVIAAYHG
                     SRTSIETRALDLPTIQKKVRKNDTGDFTLSGSSSSAFVWAALTNKYFAAIVHPVPPEG
                     QEESWLRFDRVQYYPASGADKDAHNCSFGLTSESVLLAAAGQDGACRWYSMQVYLGPK
                     DRKIFGKNPTYRALDYFQTIHFGSCCCPAWMIAPLAFGIIWLMNVLYTLMGPLGNYGV
                     IIMFLVFIVRLALHPITKSSQVSMMKMQKLAPKMQEIQKKYANNKAEMNRQVMTLYRE
                     MGISPVSGMLPMFLQMPIWIALWSAVNTSVDLRGAGFLPFWITDLSLPDHLFAIPFAD
                     RLQQIPLLGAVVPDYFNLLPILMGVVMYLQQKMMPAPQSAQTNPQMAQQQKMMMIMMP
                     LLFPIMLYSGPSGVNLYIMASIGAGVVEQKVIRKHLQEQQEAEERGKVPVTAKTGGKL
                     KKRKPKPFFKW"
     gene            4725..6110
                     /locus_tag="PLE88_02245"
     CDS             4725..6110
                     /locus_tag="PLE88_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOM75317.1"
                     /translation="MDDTIAAVSSASLPAGTAGRIIIRISGPQTWPIVEQILTPDKPF
                     QKNRIADCRVHIGEGLDIRGLLYAFFQPHSYTGQDLAELHLDVCSAAADAILKKIYCS
                     ARPAMPGEFTQRAYLNGRLDLTQAEAVAEIVAAANTAQLEAAERLLSGRFSETLSDLR
                     NEMLELLGLLEAELDFAEEEIEFISKDRACEQISRFQQRISNLLEGSIRCERIIDMDA
                     VGLAGLPNAGKSRLLNALLETERSIVSDAAATTRDVLTGILRLDGLDCILFDCAGLMP
                     ISEQTALVDRMAHQASLTALNKAALVLFCVDGSKEDIETDVQMRKQIQMPAVIYVMTK
                     SDILRPQDAAQRCEQLQQVFGGEFLPTSAAAESGLDALKQRIRMELLRLRAGDREHQD
                     RLTINQRHEQKLQETLKLLAAADDEIRRDSIETGSMLLRQSCQLLSGLEHEDISEQIL
                     DRIFSRFCIGK"
     gene            6120..7229
                     /locus_tag="PLE88_02250"
     CDS             6120..7229
                     /locus_tag="PLE88_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75318.1"
                     /translation="MAELKILTGPLRGHVFGLEKGGWAKTLGRGAENVDFRIPDAAIS
                     RRHAEFSYRDGQWAVRDLGSSNGTFINEIRIRETALLQHEDQIRCGSTVLLYSNPEPD
                     GLRAGRSDETNAKRIELEYDPGETMVAVAFDSLQSKQTARQRRRMMEAGQAALNLSHG
                     IKNILQTLRSGQDVMDDALGYRDIEQAKKAWNILKRNLEKVQKLVLDMLKFSRDETPH
                     LQPCQFNRLIESVIEILRPQAHERQVSILTQYDEHLELVWADPEQMQDVVMNLVLNAV
                     EAVPPQRGQVTIRTELDAAGQAILQIIDNGPGIEDTRVIFEPFYSTKPNVGAGLGLTI
                     ARKIIRHHGGCIEVQSLPGQGSIFTVRIPAGSDKK"
     gene            7434..8579
                     /locus_tag="PLE88_02255"
     CDS             7434..8579
                     /locus_tag="PLE88_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75319.1"
                     /translation="MNTKNTQIKMPDTFFAPAGREQPHQVRNLNARIESNPYVKSMLD
                     ALPMMVLVLNSHRQIVAFNETVLKITKSSAEAVLGKRPGEVIKCVHAGEGPDGCGTSA
                     ACRFCGAVQAVLQTQADSCRHKEECRILMEQGLALDWKVTVCPLMMDGYSLVCVVIED
                     IGDLKRRQVMERIFLHDMINHLGAIVGFTRLLAEEQGENEELKEVIQLADGLLEEVQA
                     QRDLMLAENCELEAKREPVALKSFLDRLVQLYRSHPVSAGRLIVAENVADISIETDPS
                     LLRRVVGNMIKNALEASEAGQTITVSCRQAEAGAAVSVHNPAVMPPDVQMQIFNRSFS
                     TKTGTGRGIGTYSMKLLGETYLHGQVTFESSPEAGTTFTLTLPLKSV"
     gene            complement(8597..9229)
                     /locus_tag="PLE88_02260"
     CDS             complement(8597..9229)
                     /locus_tag="PLE88_02260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOM75320.1"
                     /translation="MNSNAFDWFEIAKRLKAISQAGKHFAQNEYELNRHQELEQICAE
                     IFAHYTELNAEQARGILQQDAGYPTPKVDCRGVVFKDDKILMVREAADGGWTLPGGWC
                     DCGMTAAQNVAREVWEESGYEVKPVRLLAVFDRDRQGHTPPYPFNIYKLFFLCELTGG
                     QPTVSHETSEIGWFAEEDLPANLSRGRTLHHELKLFFAQFRNPAIPAAFD"
     gene            9515..11869
                     /locus_tag="PLE88_02265"
     CDS             9515..11869
                     /locus_tag="PLE88_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM75321.1"
                     /translation="MASAERIVRRLDFGWKFIKGDPAGAQGPAYDDSGWRDVQIPHDW
                     SIEGPYDANWASATAYLPAGTGWYRKTFELDKTLHGKFISIEFDGVYNNAEVWINGHF
                     LGQRPFGYIGFQYDLTPYVLFGQEQKNVLAVRVDHSQFADSRWYTGSGIYRYVRLCIT
                     DPVRIAHWGTFVSVPKADPQKAEIQIETTVENRGGQRQTITLLSEIIAPDGKLAASVV
                     SSRTIEAGKTSIFEQTAAVSNPALWDVDNPNLYTLKAALSIDGKPADTLETPFGVRWF
                     RFDPNEGFFLNGRNLKLKGVCLHHDAGPVGAAVPEKMWVRRLKALKEIGCNAIRCSHN
                     PPAPEFLDLCDRMGFLVMDEAFDEFTPTKNKWVKGRNVGVPSRDGYGQVFEKWSIRDI
                     QDMVRRDRNHPSIIMWSIGNEIDYPNDPFSHPVLGDNYRPNNPPARDLVRWGKPLVDA
                     VKQLDTARPVTAAIASAPMSNAAGFADILDVVGYNYQEQYYQSHHAEYPQRILYGSEN
                     STSLTAWQAVEDNDFIAGQFLWIGIDYLGESPGWPVRSWTGGLFDLCGFKKPLGWFRQ
                     SLWSAEPMVYLVCSPARRQTGQFDRRGGRMFQSHWNWPEGQTVRVACYTNCDQVELYL
                     NDTSLGVKNFADAVRRTLEWEVPYEKGQLKAVARRDGKAVCQYVLQTADKPQKIQLIA
                     DAAELKADGRDICHLEFLVTDKNGVRIYDAANQITFKITGPGKIIGLGNGNPAAHESH
                     QGKAYPVFEGRGLAIIQAGQSPGTITIEASAESLEPTKITLDVR"
     gene            complement(11873..12460)
                     /locus_tag="PLE88_02270"
     CDS             complement(11873..12460)
                     /locus_tag="PLE88_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOM75322.1"
                     /translation="MLSLFVNTYIKMFFLLAPFFTMSLFLKMAEEMDASSRRRAAMRA
                     SATVLIAVTVFFFFGKPLFGLLGITLAAFQIGTGTTLFLTSVMLVLGISKPPQHTEGE
                     DFAVVPLGLPIIVGPGTIGTLMVWGTQITNPHERLVMFAGILAAAVTMSLFLLMADQL
                     RNIFGQKVIAVMTKLTALVLTALAAQIVFTGIKNF"
     gene            complement(12474..13250)
                     /locus_tag="PLE88_02275"
     CDS             complement(12474..13250)
                     /locus_tag="PLE88_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOM75323.1"
                     /translation="MLGAIIGDIVGSVYEWKQIKTADFPLFQEACTFTDDTVMTAAAA
                     KAILYGLDYAAVYRDFGRRYPQRGYGSNFRLWLMSDNAGPYNSWGNGSAMRVSPVGFA
                     FDTVEAVLKEARRSAEPTHNHPEGIKGAQATALAIYLARCGCDKDSIRQEIRRRFGYD
                     LNRTIEQIRPTYQFNVSCQKTVPEALTAFFDSTDFEHTIHLAVSLGGDSDTLACIAGG
                     IAQAFYQKIPQWIIDQALLRLPQEFVDIIGAFEDKYGKIR"
     gene            complement(13314..14672)
                     /locus_tag="PLE88_02280"
     CDS             complement(13314..14672)
                     /locus_tag="PLE88_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOM75324.1"
                     /translation="MKMTNLILIAALLAGNVSAKVDLVTLPAKEMTQLTIYNSADLTL
                     VRDARSLTLKEGDNKLQFSWANTLIDPTSLLMQPQAYADKIDIADLTYPPRVQNLGLW
                     NIKSEIDGRVPVEITYLTSGLSWRAFYLGTLSNNEKTMHLEGYVVVSNNSGEDYENAQ
                     TRLIVGQVHILDQIAELARRQYPYGRPGEVFPAKPASAIEEPRALAGRARRELLALDA
                     AALQTKEIRKEGLSEYFLYTIEGTETIPDQWSKRLPSFQADQIPVVNLYKFEEDRWGP
                     QVVRYLSFKNDQEHKLGQTPIPGGSLKVFRSVDDQQHLSYEGQSSFKYIPVGEDVELN
                     LGPVSNVIVNPILMDEKTDNYRFDKDGNVNGWDEIRTFKLELKNTRPLPVKLELKRNF
                     PTSYWTIKNLGDADTYYEKVDLDTIKYTLTLDQQTEKTIEYVVTYYQGVRSEDWTRMQ
                     RQ"
     gene            complement(14760..15902)
                     /locus_tag="PLE88_02285"
     CDS             complement(14760..15902)
                     /locus_tag="PLE88_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75325.1"
                     /translation="MKAKTILTSIAVLAVAAAAHGRTKLVALPQRDATVIRLDNPAAT
                     LIEEERVLTLQKGLNKVDFSWNAVSIDPDSIRLTILDHPEQVKLLNVSYPPNEAALVW
                     EIAAEGDYAETVRISYLLANIDRLVTYKAVADKDETKLDLKSYLILRNFSGEDFDKAA
                     VLLDYGDSFEQSIAHEETKQLLFLKAPEVPITKIWVWDAAKLPWDPEQVENRNIGIPV
                     SYRIVNDKKSGLGDFSLWGGKMRLFQDDGHESTIFLGEDFTSIVPVGQKTEVYIGDSR
                     DIVVTQRQMKNDRINLRKNNAGAIVLYDTDEVITAKIENFKDKPAVLTMIQHIPGQWE
                     MKNCNMEYTRKDASTLEFEIQLPARTQDGPAVKELEMRYSRINVRP"
     gene            complement(15929..17185)
                     /locus_tag="PLE88_02290"
     CDS             complement(15929..17185)
                     /locus_tag="PLE88_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOM75326.1"
                     /translation="MKPKALFMPAAVLLMAAGICWAEFQPYSKAIVEGNTAFALELYA
                     QLKNQDGNLFFSPFSISTALALVYAGAKEQTARQMAQTLHFPFDTEPNALELSEPFHP
                     AFGRLITQMNRQGQKGDYLLSIANALWGQKDYPFLDSFIMLNKNFYHAGLETVNFISE
                     TEAVRLKINQWIQDKTQDKIKNLIPAGALSAATRLVLTNAIYFKGSWAEEFDPKLTQN
                     EPFYISHDRTVPAPLMHRRGKYRYGEHVSFRTARGIKVETLELPYKGGDLSMVVLLPE
                     KGYAGRFEEDLTAKLLTQWMNSMQMKVVDVYLPRLKIESQFSLSQTLAAMGMPDAFGS
                     TADFSGITGTKDLFISDVLHKAFVEVNEEGTEAAAATAVIAPTAAVPEPPLVFRADRP
                     FVFVIKDNKTGSILFIGRIMNPVQEQ"
     gene            complement(17182..19065)
                     /locus_tag="PLE88_02295"
     CDS             complement(17182..19065)
                     /locus_tag="PLE88_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75327.1"
                     /translation="MNHFNQDILIQYAFGLLEANAGLAVRQHLDKCPECRQHLSELKR
                     KFSVMSVLEGDVELPDDLVSKTLMSKMAGEKIGAFYRKVWIGWAAAAALVVTALWIFL
                     PDSQNKIGAADRFGPKKREQFKDSRDYLSYTKFIQDGAQPVQNSVKIVSSDSIPDSPP
                     FAPASAIELVVLPAPDTMQVTIYNTADLTLVRDIRRLTLKPGWNWLQFMWAETLIDPT
                     SLSLQPLEYADKIDTQQLVYPARLKDIGRWLIRSEVEGAVSFEITYFVSGLSWRAFYE
                     GTLNEDETTLDLKGFVNVANSSGQDFQNAQTRLVVGQTRLTETINYLAGRYYPYGPDV
                     PQQDEKYIYKDALKKVPLLADFAQTGMLFSDTSGRFGGGAFKYEIKDIQKEALSEYYL
                     YTIEGTEDLPNAWAKRLESLDASGIPVKSLYKYDEDRYGTDTIRFISFKNDAAHKLGN
                     TPLPEGNVRLYRRLNEKKNLSYIGSAEVKYIPVNEDAELNLGAALLVKVEPIQIDSRT
                     DNYTFNNNGDIDGWDEIESWQLKLTNTRQIPAEIEITRNFGTDYWELKIDDYWESGIN
                     ANGNSTPHAAYTKHDKSHARFTLVLAPRSEKTFGYTVTKYQQRRSEYYVEKMTAQSKE
                     QEK"
     gene            complement(19062..19649)
                     /locus_tag="PLE88_02300"
     CDS             complement(19062..19649)
                     /locus_tag="PLE88_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM75328.1"
                     /translation="MDDSDRNLLDAFRSGQTGAFETIVRRHGPAVLGYLTKMTHNREQ
                     AEDLFQETFQKAHKYAAAFHGDNLRPWLVTIAARTAITRRQQEKKHTAVSLSQPICAD
                     GIHCGTLETTLPAGAPQPADQLILDEQRRQVRDALGKLPEQQRTALILNYYHQFTHQQ
                     IAEVLNCSVGTVKTHLFRALKKMAALLPDPAGENQ"
     gene            19869..20531
                     /locus_tag="PLE88_02305"
     CDS             19869..20531
                     /locus_tag="PLE88_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75329.1"
                     /translation="MKGMFLSAAASIFAFVSYASWQNPVFLQELNDFQNGYAANRPMV
                     SSDGMLLYFVRSTPTKIDALWEARRNPETGVFEHMRQLSELKYGGAQIYGTWLSPDNL
                     RLYYAASDPVALGWGKRPIWLATRTSMNAPWQLVRRFTELEIFNFQTSCSLTADEKVI
                     MWESVPFAEGGLKRIFVGMRPSILHSFSNFREAIELEDIKAYVPRMSPDGLSVYFKKI
                     EQ"
     gene            20521..20802
                     /locus_tag="PLE88_02310"
     CDS             20521..20802
                     /locus_tag="PLE88_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75330.1"
                     /translation="MNSEGIIEAWLGHRGSLDAPFGNFEPLEEINKTGIGADVCPAWN
                     GQTLFYFQRQGEAGDLNATGIFVSHWVDDSYTDALVVLGRAVMKKRKGY"
     gene            20807..21028
                     /locus_tag="PLE88_02315"
     CDS             20807..21028
                     /locus_tag="PLE88_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75331.1"
                     /translation="MTQAGTDERKALQILSILPASDLPAELASKSRWDAVKHIHQSIK
                     KQEQVVGVLETCITNLQTALWLLSPLSIP"
     gene            complement(21086..21913)
                     /locus_tag="PLE88_02320"
     CDS             complement(21086..21913)
                     /locus_tag="PLE88_02320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM75332.1"
                     /translation="MNYPPGHYYSPLPDISWVNAHQKILYPRDVKQAAGIELRTIEQQ
                     KLLKDLFVYFAEFDFPDDPSDKVRYYCHNPMYGKGGALILFGIIRRFGPKRIIEIGSG
                     HTSALMLDINDRFMNRQIKLTFIEPCPDRLYGLLKDEDKAHCTVYETQAQNIPSSCFE
                     QLQENDILFIDSSHVAKIGSDVNHIFFNILPVLNDGVIVHFHDIYWPFEYPIEWINEA
                     RAWNEAYLLRAFLQYNNRFDIILFNNYLARCFPEFYQECTGSVGGGSSIWIQKKTSR"
     gene            complement(22044..22619)
                     /locus_tag="PLE88_02325"
     CDS             complement(22044..22619)
                     /locus_tag="PLE88_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOM75333.1"
                     /translation="MLKDILLALIPMFVAVDPIGVLPIFVSLTEGLSRLEKRRIIIQS
                     MLTAAAVAVGFICVGKGVFHLLGITIGDFMAAGGAILFCLAITDILNPVKARRNPGEP
                     LGAVPLGTPLIAGPAVLTTSLLIISEYGIAATLISVIANILLAGIVFAASDILLKALG
                     EAGTKAVSKVFSLLLAAIAVMLIRKGFQQFL"
     gene            22804..23325
                     /locus_tag="PLE88_02330"
     CDS             22804..23325
                     /locus_tag="PLE88_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75334.1"
                     /translation="MARRIAAIAFIYACTAAAWMILSVTMLVRTESLDTRLHEAVGQL
                     WGTAHTQTQPQVYYKIPSSSSLSESNEQNETVEHLALDSSQIAVDLKLDYRRKGLLWY
                     SVYQVQFAGQYRIANPGSQQREIYVDFAFPSEQAIYDNFRFAADGQDITNSAFKRAAL
                     SMLFRWTPAKRRK"
     gene            23298..24071
                     /locus_tag="PLE88_02335"
     CDS             23298..24071
                     /locus_tag="PLE88_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HOM75335.1"
                     /translation="MDPGQTKEIEIAYTSQGMDEWIYDFGGGVKQVRNFVFVMTTDFD
                     RIDFPQRSVSPTVKAKTAKGWKLVWAYNNMLTGACIGMTMPKKLNPGPWVSRLTAAAP
                     VSLFLFFFLLFICTTLREIKIHPMNYFFLGAAFFSFHLLLAYLVDFISIHIAFWICSA
                     VSIVLVVSYMRLVAGPRFAFLEVALFQLVYLVVFSYTFFFEGYTGLAITVLCICTLFA
                     VMQMTGRLDWEKVFANGLPKPAKSRKPQDGGQNRPNVPD"
     gene            24266..24700
                     /locus_tag="PLE88_02340"
     CDS             24266..24700
                     /locus_tag="PLE88_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM75336.1"
                     /translation="MNKSIKILVVDDDAVARGVLIHYLSSYGSCTEAENGAKAVEQIR
                     QAIIDKAPFEVVCLDIMMPKMDGLDTLQAIRQIEKENQIEPANQAKVIMTTTASQVSK
                     TMKAFHYGCNAYLVKPVSKDDLYREMMKLPVSERYKAYWRTL"
     gene            complement(24717..27062)
                     /locus_tag="PLE88_02345"
     CDS             complement(24717..27062)
                     /locus_tag="PLE88_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM75337.1"
                     /translation="MKTLVTAAAVCCFLTSIASGDSLALPYPYAVQPVSPPSVYAFGL
                     SDVHLTEGIFKDAMNRNAQWLLKLEPDRFLAWFRKEAGLEPKGKVYGGWESQGVAGHC
                     LGHYLSACAMMYAASGDAQYKARADYIIDELALCQEANGNGYLAAYPNGKRIFEEIAR
                     GDIRSAGFDLNGGWVPWYTNHKVMAGLRDAFVYCGNAKALEVLKKMGDWTFEVTKNLS
                     DEQWQKMLACEHGGMNEVLIDLYAMTGEEKYKILALKFYHKEVLDPLANQKDSLAGRH
                     ANTQVPKIIGAARIYEMTGMENFKTIARFFWDTVVSHYTFVNGGNSANEHFGQPDKLS
                     EPMHDTTETCNTYNMLKLTRHLFAAQPRAAEMDYYERALYNHILAHQHPRTGMIMYKG
                     FLDMPARKHFCDPFDSFWCCVGTGFENHAKYGETIYFHNGNALYVNLFIPSVLTWKDK
                     GITLTQETAFPARDTVKLTIDCPKTTTFILKLRKPAWTTGMTVSVNGKPVSTEPDSTG
                     YIAVNRGFNRGDVVELKLPMTLRTEAMPDKPNRIAFLYGPIVLAADLSGDAPLPLLVG
                     TPAELLAAFKPVEGRPLEFTAAGIARDIEDEGAMRELRLLPLYAVADQPYTVYMDQFT
                     PEQWQQKKTEYAAEQQRLQELEARSVDVLRIGEMQPERDHNLTGENTQAGEFAGRKWR
                     HAYNGWFEFTMKVLPDVPMELVCTYWGSDAGARTFDILVDGTKIASQSLNNQKPNEFF
                     DVTYPVPSELTRDKTSVRIRFQAQPNNTAGGLYGVRIMKVK"
     gene            27703..29706
                     /locus_tag="PLE88_02350"
     CDS             27703..29706
                     /locus_tag="PLE88_02350"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HOM75338.1"
                     /translation="MKTHLFSYLILVMLLVLCCASVTAYEVSAAGACSSPNSHPDAKG
                     FYDKVRSFSGWTGNFYVEDNNSKEIHYKRVDMGGQNNSWVDSSDIHYHVSHGGTRWDS
                     YYGKDLKAVLFEDGTSLVPSEARTCWGDSDLEWIAFRNCQLLNDVSRPYWANAMNGLH
                     LILGFVTNSSKHDNFGKLWAEKMKKTTILWWTIPGQTITQAWFNTADATQPSGRIARV
                     LAEVQDNYNDHLWGNGYVSPDPPVDNYYWYWDHTAGSPPYLNVNMLEMMYIYKVMPRT
                     VNEEYVQQIGNAFGLQGPIGQNCESYFMADLSNPAGPRILEISKTTGHFHFHDDGKLF
                     VLDPQGGKFPPEQALEVAQAFLSRNKLLPQDARAYAVEYDTLTEENYEKGTIRQRLTM
                     NTNVVWARQIPADPKQTLVSVAGAGARLKIYLDKTGGIMGGLGNWRQIQPIELLEVND
                     SRKTWSFFDVFGSKIVLEPPLVEYDQAVPNFETAVQLYYEFSYDIQQAELIPCWLFNV
                     DYYLKGKLMLTAETFIPASTMYMAPVVEIVKPEDSSKFPAGSMIGFDCEVASGLGTPP
                     YSFQWTSSVDGVLSDQQTFQTDTLTVRCPDISMDCQPLPHTITVTVTDAKGLQSSDSI
                     QITIIGPCDECKDPADFNQDKIVDLEDFAYWASRFLMQSGKQE"
     gene            complement(29801..30883)
                     /locus_tag="PLE88_02355"
     CDS             complement(29801..30883)
                     /locus_tag="PLE88_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOM75339.1"
                     /translation="MMTKTMMMGILFLASFASAGIYSGGGSGTADNPYQISTPADWQI
                     LMSTPQDWDKHFKLTGPLDLAGVTLTPIGNSTTKFTGVFRGDGWTISNAVISQPGSDN
                     VGLFGYIWGEAEICDLHVENAFVEGRFGVGGLVGRLDSATIMACSWNGTVKGVSLVGG
                     IAGCCTLQQSAFLGCWSMGTVTGSRYVGGLVGYLGGGTLYYCYSQASVIGVIDTTGGP
                     NEVLGGLIGWMLSSNSLAAKCYAAGIVQRPSISIPNVGGLVGLNDGSIVADSFWDMQV
                     SGESTSAGGIGKTTAQMKQSTTYISAGWDFVNIWELGEHQTYPYIRSRPSADLNRDGV
                     VALEDFSILAEQWLTEDIQAPSELNV"
     gene            complement(31132..33300)
                     /locus_tag="PLE88_02360"
     CDS             complement(31132..33300)
                     /locus_tag="PLE88_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM75340.1"
                     /translation="MKQTKLFLMMLLLMVPVYGDIVGSDFFNYPDGPVAGRNGGYGWD
                     WKGVGKPQGPSPTSWLNQAGITNVVNGTLSLQDSGAGWISARREFGSDTWGTGAFQNV
                     GIVYFAATLTINAPQGWLGISAQDGEWGERVKFGMPWQTGGLGYLGVNNENSGQYVLT
                     NVQAVIGRPYRIVGVLDYNHNAARMWVDPDTGDYDIAWNHTSADAVFNNLNMWNWVNG
                     VRIASGAATTWDDVIIATTFPETFVPGSLSDRPHNPQPASGETDVPITASFVWTVPQD
                     PNGQADPNLVSMKLYMKTAAEPNFAFAADILVWDAETLQASYTPSTLSRDTTYYWRVD
                     TIYNTASVRTGDIWIFETEKSVPIITGHPAFQIIETGGTATFTVTVVSISTPTYQWFK
                     YVDGQNDIQLSDGGDISGVTEAALAIANAKMSDEGLYYCIVNNESGIPAVSGKAPLAI
                     KRRIAYWPFEENNPNSTVPGSPVSLLYGDPVFTAGIVGSGMEFDNNTGAEDMLYTDPD
                     QSSYFDICNYSITVACWIKAPFAANWGPMVARNGESGQGWQLRHRGDTLNKICFTTRG
                     TANDDGTASNRTVYDNSWHYAVGTFDGSVKKVYIDGVLSRVYSRDDGRIIQEYDEAAG
                     VINSTLSPVSLAGRVRGSSGNLIFEGSSVTPCVLDEVEIYNYPLDAATIAQTYADITG
                     KAVCPAPVAYDLDGDCIVDLGDFVKLASEWLLDTSVQPAQ"
     gene            complement(33870..34364)
                     /gene="ilvN"
                     /locus_tag="PLE88_02365"
     CDS             complement(33870..34364)
                     /gene="ilvN"
                     /locus_tag="PLE88_02365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOM75341.1"
                     /translation="MKHILSALVLNKPGVLAHVAGMFAARAFNIDSLAVGRTDDPTLS
                     RMTIVIIGDDKVVEQVRKQLLKIVEVVKVQDFVGQDVVARDLMLLSVACTPEKRPEIL
                     ALIEMFTGKVVDIGPKYVMVEVAGPESKIEAFIEMCRPYGIKNIVRTGTVALARQSRT
                     KQED"
     gene            complement(34468..36186)
                     /gene="ilvB"
                     /locus_tag="PLE88_02370"
     CDS             complement(34468..36186)
                     /gene="ilvB"
                     /locus_tag="PLE88_02370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOM75342.1"
                     /translation="MRKNGTPSDDGKVIKVGSQVIVDMLLEQGIDTMFGYLGGVVLPL
                     FDKLYEAPIRFIIPRHEQGGCHMADAYARATGKPGVVVATSGPGATNLTTGLATAMMD
                     SVPIVALTGQVRTSLIGNDAFQEADTTGITRPITKHNVIVKDPNTLAQTIREAFYIAT
                     TGRPGPVLIDIPVDMQLAQCPIPPKGEIELPGFKNRQKGHSRQISAAAAAINQSQKPV
                     LYVGGGAILSGASEILRAVAQKANIPVTMTLMALGAYDQTRPESLDMLGMHGSAYANY
                     AVQNADLIISVGARFDDRVTGNLATFAPHAKIIHIDVDPANIGKNLHVDIPVVGDAKQ
                     VLEEMIGELEYKQRKEWFAQIAAWKAKHPFTYDRKTDVIKPQYVIEEIYRQTKGDAII
                     CTGVGQHQMWTAQFYKFSRPRQLITSGGLGTMGYGLPAAIGVKIACPDKLVIDIDGDS
                     SFNMTLTELSTAVMYNLPIKVALINNGYMGMVRQWQELFYGGRYSQSSLVNPDFANLA
                     RAFGCVGITVEKKEQVPGAVEQMLAETRPCVVDFHVDRQENVWPMVPAGKSLHEMDGL
                     DIFEMA"
     gene            37183..37959
                     /locus_tag="PLE88_02375"
     CDS             37183..37959
                     /locus_tag="PLE88_02375"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOM75343.1"
                     /translation="MKSPDQKRIQQVWDHFLQYHDDVSRNILLEHYLDLVKYTAQRLS
                     TRFPKSIDLADLYSAGILGLIDAISKFNPSRNIKFETYCVRRIKGAILDDIRRNDQLS
                     RLVRARAQQLQKVNQQLQTLYGRMPTDEELAAALGLNKKAFYELLRDANAGCLISLNA
                     GFSSAEGDETFGELSIYANQKSQDPFLEIQKRDLREFLFKGFSSEERIILKLYYLEEM
                     TMKEIGKTLGISESRVCQIHSSVIARLRARFNQYMQVFTV"
     gene            38109..39488
                     /locus_tag="PLE88_02380"
     CDS             38109..39488
                     /locus_tag="PLE88_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOM75344.1"
                     /translation="MDSSIFKAYDIRGLYGEQLDEQAAWKIGHATAQFLRSMVHGYNR
                     GLANAQSICVGRDMRTHSPSLAKSLIEGMNAAGANVIDIGMIDTPQMYFAINHLGVCG
                     GVQVTASHNPAAYNGFKISGQMARPIGADTGLNDIKHIATALLHTLGRPNGSVEHCDL
                     TEEYKRHVLKFLDSKHRPLKMIIDASNGMAGKMVPLIFGQLPNLDVTVLNKECTGTFK
                     HDPNPLIEANLRELKETVQKQNADFGVCFDGDADRLVMVDEKGQPVPCDIMTALMVPY
                     FLKKNPGSTIVYDLRSSHVVQEEILKYGGIPRRERVGHAFMKKTLRDSHACFGGELSG
                     HFYYRDNFYADSGMITLVHLINILGATSQPASEMIAPLKRFYSSGEVNFEVEDKAGMM
                     KELARKFGQGQVDDLDGITVQFKDWWFNVRPSNTEPLLRLNVEAKTPELLEEKFAQLK
                     SILGQPVAH"
     gene            39659..40843
                     /locus_tag="PLE88_02385"
     CDS             39659..40843
                     /locus_tag="PLE88_02385"
                     /inference="COORDINATES: protein motif:HMM:NF027641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOM75345.1"
                     /translation="MKSMQWYVVEKRQMVAVFLFLIVAAVYGVGQPANGEQMQHFSNK
                     VQVGAYYYPWYHAASDERKGQWSHVMRLHLNPPQRPRCGLYDSADPNVIRDHIAQSMQ
                     AGLDFWAVSWWGPQSLTDNVFKDAVLKHPEAGKLKYAVLYESTGRLGSFSKPSYRNWI
                     SDLKYIKETYFNNPCYLTIDGRPVVFFYLSREYFRNKGQEALAQMRKEIPELYLIGDD
                     VFFAGGASEEYKPEWAANFDAVTVYDVYGQSIGRYGNTQKAVEILAANYRQAKQAANS
                     VGVGFVPTIAPGYNDTAVRKGHPGRARYFADADDCKEGEVFRQMIRKAALPNADVRCG
                     RIIMITSFNEWFEDSQIEATAGIQPETAADDSPDGKTYTGGSVYRDYGTLYLDILREE
                     IK"
     gene            complement(41264..41641)
                     /locus_tag="PLE88_02390"
     CDS             complement(41264..41641)
                     /locus_tag="PLE88_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4174 domain-containing protein"
                     /protein_id="HOM75346.1"
                     /translation="MAAQTFQSNRVQDGPTFLFVFSPSRQDHLYQIQMETLAERQRTL
                     EGHNVVIAEVFEHEPGHVGSDELHPENSHELRRDYNILPGQFKVVLVGKDSNIKLRAE
                     SCVSCEEVIMRVENEPIKMESAF"
     gene            complement(42030..43019)
                     /locus_tag="PLE88_02395"
     CDS             complement(42030..43019)
                     /locus_tag="PLE88_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM75347.1"
                     /translation="MKLISKDTGYANTLGRYSAVGRSVAFQSVMETVRIVAPRNCCVI
                     IHGPTGTGKEMTARQIHLHSRRANNVFVPVDCTALSGSIFESQLFGHVKGAFTGAVSD
                     TLGFFRAADRGTLFLDEISELEPDLQAKLLRVLQESRVTPVGSTQSYPVDVRIICATN
                     RDLKEMVRQGTFRVDLYFRLNVFKIELSPLCQRKEDIPLLANHFLQKQAELYDEPVKR
                     LSEQALKILMDYHWPGNIRELANAIEYAHIASRGPVIEPEYLPIDILTGDFLTADSQD
                     PEFTAFDQIRKRMVIKALQKAGGRKMAAARLLKIDHRKLSRLVKEFGLEPTWK"
     gene            complement(43241..44302)
                     /locus_tag="PLE88_02400"
     CDS             complement(43241..44302)
                     /locus_tag="PLE88_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM75348.1"
                     /translation="MEDPCSNCLNLDKRDSFLRDNPHFFNYISGSPENIFFTQSIGNP
                     ATDQILALFESSSDCILIWDKDYNYLYANQAAIEHVKTTRDKVIGKNIRDGLGHIPDF
                     MRLWMSRIDQVFATGQPLQVEDAMPVGDRLVYSQSVISPIRNSNGQMIAVGVIYRDIT
                     EQKTLEKELLENEKKYRQLYEQAQIPLYRTNIKDGKLIECNSALAVLLGYPSKQDCLD
                     KCNATAHYADPKVRIKLLELLKEGPIKRFETEIIRCDGSRAWVEITAQLYAEKDYIEG
                     AIYDITVSKILTKTELQILLYILEGKSNKEIAKIIGRSIRTIEDHRSHIMQKMNVRTL
                     VELVQKCQFLSLSLKKINS"
     gene            45047..45406
                     /locus_tag="PLE88_02405"
     CDS             45047..45406
                     /locus_tag="PLE88_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75349.1"
                     /translation="MREDQSFSYAWIAALLENTTAAVKQHFSLLGKELHWLVFSRRVL
                     EPIVHRTDPPSLAQLCKEYALENETVASNMITTVKRAFRKALRTRIREYVYTDSNVDD
                     ELTDLMVFLRKKSQDGG"
     gene            45502..46035
                     /locus_tag="PLE88_02410"
     CDS             45502..46035
                     /locus_tag="PLE88_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75350.1"
                     /translation="MNKEMLHLLKPDQLIEMLSIGMDGDPPNSAELVSDEQIRDYFCH
                     LINRKIPCQSSIVDSILIFLRENERYGHLVKGRTLSELLLDSSCPAGLFEAVKEISKR
                     VCQTAISRGENAVASAIYYAAIAAALIYCNTKISSHSWETLKTSFEDLLAKSWTGPFS
                     ELLEKARQRCEKEAAMK"
     gene            46032..49097
                     /locus_tag="PLE88_02415"
     CDS             46032..49097
                     /locus_tag="PLE88_02415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOM75351.1"
                     /translation="MNPNLPFDPQPSPDDALPISQTAGGQEPGFSLPAPKIDGYEILH
                     KIDEAGQGQIWAAVQQSTSRKVAVKVPRFQGFGARKAFLRFEREVQVIARLKHPNIAR
                     VYDSGVNRGFYYYTMEYIEGRHLDRYAAEEKLSRRDILMLFERVCRAVEYAHQNAVVH
                     RDLKPSNILVTADGQPHIVDFGLAKEMDDLTVTISMDDLPVGTPAYMSPEQAEGRPVD
                     TRSDIYSLGVILYRLLTGTFPHDMSGSRQEVLRRIAQDDIIKPVFLTQKPDKELEYIL
                     RKALAKEAQRRYSSVQELAQDIENYLAGRPLLAGPESAFYIVRKFIWRHRTLVLGLAG
                     GLSALVVALVAISLLFWQAQKAKREIQQALEREERLNYRNTIQMAHSLLENSRYAGVR
                     RMLEDTYPSKRSWEWGYLMKYCQLPEKSLSLDIAVDTAALSTDGRFLILSDSLTQTAM
                     LMDSREGTVVWQQKIPGYITGFVQAAFDPSNRFAAVASGTELVVFDIADGHILFKSKG
                     IGFLSCCAGPAGLYAGTFDGHIFLYDTQNWQVLQTAVLKEKISLLAIAPDGTQLAALS
                     GMRTITLHDPHTLQQQAALTAEDSVYQDLLVLSAHTVASASGNGFTIDHLTTAPQERS
                     AGPIRDHLQEITALALSASQDKIITGGLDGVAHIYDARTGQMLATIYHDSPIQKVFFL
                     PSDKVITLGADGRIKWWSGCKHEPQHPDVTISLPLSAGVWQLDYDSDGRHLVFATSDC
                     PQIVLADAQTGSTRAVEAEGYSEEIKEFSPRVFFRPGKSELILKIKDSLRIYALKEDR
                     FEEIRRFPLSAPVGSAAVDPKGRFAAVAYLRGFGILIDLDAGSIRQLAYGNLAASYAV
                     FSPDGSRIVFLEATGSKRAAIIDSQTGRLVCTLFADFRRQAQVAAFHPNLPILAIASS
                     DGVIQLWNIDTSRKIAEMTENGLGAVYGLQFSRDGQRLLSGSHDKTIRLWDWTIGSVL
                     LKISHPATPVDVCFSPDGLSIACAYYMPNSCRIYRAIPLAVLQGSQSEQ"
     gene            complement(49166..50497)
                     /locus_tag="PLE88_02420"
     CDS             complement(49166..50497)
                     /locus_tag="PLE88_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOM75352.1"
                     /translation="MSKTININVHHITRVEGHGNIVVNISNGTIEKCQWQVPEAPRFF
                     EAMVRGRRYDDIQTIVSRICGICSISHSLVATKAVEDALGLKVTEQTDLVRHLMHYSE
                     QLQSHVLHVGYLVAPDLFGEKSVVPLVPKATEAVKKIIKAHRVANQMSALLAGRITHP
                     VTLTPGGMTRIPTEQELRDLKKTLEDVVPDLVDICKVVLSVAGNLPAFERPTEYVSLR
                     QTNPFYKNNLSAYSFYHGDITSTDLNGKADMPVNQWENVANEYVVPQSTAKWAKWHRD
                     SYFVGALARFNNQAEYLSPLGKTVADMFGLKKGCCNPYMNSVAQLAEAAHVVQASLEL
                     IDILLTKGLKPEKLRVSPRAGRGYAAVEAPRGILFHAYEFNKKGECTWANCCIPTNQN
                     HANIQNDFEKLVPQYMHEGQDALRQKLEMLVRAYDPCISCSTHYLHVEFVK"
     gene            complement(50514..>50750)
                     /locus_tag="PLE88_02425"
     CDS             complement(50514..>50750)
                     /locus_tag="PLE88_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75353.1"
                     /translation="CVECKARETICRYEYNEICLGPIIRAGCNAPCPAAGFRCFGCRG
                     LVDNPNLQAAQEVMDKYDLTVDDLKQKMVLFNTK"
BASE COUNT        12840 a        12060 c        12713 g        13137 t
ORIGIN      
        1 cggcaaaaag aacgcttcca gtgaattgca ttcgccctaa cccttaccag ccgagaactt
       61 catgggatga agaacaatta aaggggcttt gcgagtcaat tcgtcgaaat ggaattatcc
      121 aaccgattat tgttcgtaaa aatacagaag gctatgaaat catcgccgga gagcgccgct
      181 tcagagctgc ctgtatggca gggcttcaag aagttcctgt ccttgaaagg gcggctactg
      241 atgatgagat gctcgaatta gcacttgttg agaatattca tcgttctgat ttgaatccga
      301 tagaaagagc aaaggcctat caaagcttta taaatacatt caacctgaca cagacggaag
      361 ctgcccagcg cttaggcgaa gaccgtacag tgatagctaa ctatttgaga ttgctggact
      421 tgccgacaga tataaagcaa atgctaagcg gtggacagct tacaatgggg catgcccgtg
      481 ctattctttc gctgccttcc gatgatctga agcgaaaatt ggccaaccga gcaatggcag
      541 ggcgtctgag tgtaagagaa gtggaacggc tagtcaggat ttatatagaa aagaaagatg
      601 agaagctatt aaagaaggaa aaatcgcccc atatagccga tttagagcga agattacagg
      661 aagtgctcgg gacaaaagtt aacataaaac ccaataagcg aggtcataga ggaagaatca
      721 taattgatta ttattcaatt gatgagtttg agagattaac agagaaaatg gggcttacct
      781 ttgaagatgg cgattaagac agacaataat atgttaaatt atgcagtata tataccgacc
      841 agtatgtata atataagata taaaaagaaa tgaggtatgt atggtaaaaa aaagcccttt
      901 tgaggctgtt tacaggcaat taggagccag atttgatata ttctacgatt ggctaatgcc
      961 ggcagatttt ggagacatcg aagcagaaaa taacgcgatt aaaaatcatt gtgcagcagt
     1021 cgatttaagc tgtttcggca gaatcagcgt aaaaggcggt tcgaataagg aggttttaag
     1081 cagcattttt aaatacagaa gcggttcatt agcattagat aggtggatat gggctaaaac
     1141 aacaaaaaat ggcaaggacc tgctgtgccg aattgtcaga acaaacggag actgcattgt
     1201 gataacatcc cccggccaaa atgaaatggt tatggagttg ctgaccgtcg aaggggggga
     1261 agcgatttct gtatcggatt taacggaaaa gacaggaatg ctcggcttat atggtcccgc
     1321 agcagcaaag tcagtaagag gccttttgcc ttttgaattt gatagtttag aaataggaga
     1381 tgcagcgcag ttaacttttt ttatgatgtc attcacactg cttcgcggca gctggctggg
     1441 gcaggatggt ttagagcttg tttgcccggc ctcggcggga gcaatggcgg ccggagcaat
     1501 cgttaagtat cggaacaaac acaacataac acctgccgga atggaaagct tgagccggat
     1561 ttttgccgat gttaagccgc ctgtttagaa aaaggattat cggcatcatg tttcatttag
     1621 gattgggggc tgtttttcgt ggataaccgg ttttttttgt gttataaaat gcgggtctgg
     1681 aggtatctcc gggaagtgag ttaacaacta caggcgggtt ttatgtttgc gattataagt
     1741 gatatacatt cgaatctgga agcgctgacg acggttttgg aggatattga gaagcgaggc
     1801 atcaagacga tttactgtct gggcgatgtg gtcggctatg cggctaatcc tttggaatgc
     1861 ttggatttgg ttatcgccaa gacagaaaat tgtgtgatgg gtaatcatga ttatgcggcg
     1921 atgtttgaac cgacgaattt taattcgggc gcagaagctg cttcatactg gacccgaaaa
     1981 gttctcgaag atgaaacaga ccggcagaaa agcgacagac gctggcggta tctgggcaat
     2041 caaaaaatgc ggtggacact ggaaacacgt ctgggcgatg ttgatgcgac gctggatttt
     2101 gttcatgctt cgccccgcag accgattaat gaatatgtgt ttcccgatga tgtctataca
     2161 accaccggaa aaatgagcgc cttatttgag cgggtcaagc atatctgctt tgtgggtcat
     2221 acccatttgc cgggtgtgtt tctggaggat ccggattttt attcgccgga tgaactgggc
     2281 ggtacatatc ctatcatacc ggatgaaaag gccattatta atgtcggttc ggtcggtcag
     2341 ccgcgcgacc gcgacaatcg ggccagttat gtctatgtgc aggataatat tgtgaatttt
     2401 gttcggttgg aatacgacta taagacggct gccgaaaaga tttatgctat accggagctg
     2461 gataacttcg aaggcgaccg tctggccgat ggacgataag agaatcacca gtaaacagct
     2521 tcaagaataa ttctgccggc ggtaaaagcg ttttgtcatt aaaggattga tttgaatgaa
     2581 ggcaaagata gccattattt cttgtgcatt cttctttgga ttgtgtgtat ggctggttgt
     2641 cgatgcggtg aagcatcacc cgcagcaggc gaaagttttg ttgagccgcg ttgtgatgct
     2701 gacaggcatg gttgaacagg ccagcgagtc ggccagttct gatgatgtgt ctcttgcgca
     2761 gaacaagaat gagccgacgg tttatccgca ggtatcggct gttagcggca gggaacagac
     2821 ggcggtgctg ggggcgcttt atgaggatat aaggcgcaca gagaacccgg acaagtacaa
     2881 attccagata actctgacaa cgcggggggc ggcggtaaag aatgctgttt taagtgaatt
     2941 caaagcccgc aaaaaagccg aatctgctga attgttaact gaccaagccg actgcattaa
     3001 accggccgat aatcctttga ttcttttgtc agagatggcc catgaacggc gggtatactc
     3061 actggcaaat acagaactga aacttgcgcc ggcagaggcg gagaacttcg gaacgaaggc
     3121 atttccgctg gataagctga attggcggct ggcgaaggaa ccgtctgctg accaagcctg
     3181 ctttgaagcg gttttaaatg atgcccagac gggggagccg ctgctgcgta taaccaaaac
     3241 ctaccgggtt cagccgggca gctacgatct cgactgccgg atttcgattg aaaatctttc
     3301 atccaagccg ctcaaagctt tgatgcagat gcaggggccg gcaggaatcg gcaaagaaga
     3361 ggagcggcag gatatgcgcc gggtgatagc ggcttatcac gggtcccgta cgagcattga
     3421 aacccgcgca ttggatctgc cgacaattca gaaaaaggtg cggaaaaatg acacaggcga
     3481 ttttacgctg tccggcagca gttcatccgc ttttgtctgg gcggctctga cgaacaaata
     3541 ttttgcagcg attgttcatc cggtgccgcc ggaagggcag gaagaaagct ggctgcggtt
     3601 tgaccgggtg cagtattatc cggcctccgg ggcggataag gatgcgcaca actgctcatt
     3661 cggtttaacc agtgaatctg ttttgctggc ggcagctggg caggacggcg cttgccgctg
     3721 gtattcaatg caggtttatc tggggcctaa ggatagaaag atttttggga aaaatccgac
     3781 gtaccgcgcg ctggattatt tccagacgat tcatttcgga agctgctgct gtccggcgtg
     3841 gatgattgca ccgctggctt ttggtatcat ctggctgatg aatgtgcttt acacgctgat
     3901 ggggccgttg ggcaactacg gcgtgatcat tatgtttctg gtctttattg ttcggctggc
     3961 cctgcatccg attaccaaga gcagtcaagt ctcgatgatg aagatgcaga aactggctcc
     4021 gaagatgcag gaaatccaga aaaagtatgc caataataaa gccgaaatga atcggcaggt
     4081 aatgacattg taccgcgaga tgggtatttc gccggtctcg ggaatgctgc cgatgtttct
     4141 gcagatgccg atctggatag cgctgtggtc agctgtcaat accagtgtgg acctgcgggg
     4201 tgccggattc ctgccgttct ggataaccga tttgtcgctg ccggatcatc tgtttgctat
     4261 tccctttgcg gacagactgc agcagattcc gcttctggga gcggttgtgc cggattattt
     4321 caatctgctg ccgattctga tgggcgtggt aatgtatctt cagcagaaga tgatgccggc
     4381 gccgcagtcg gcccagacca atccgcagat ggcacagcag cagaagatga tgatgataat
     4441 gatgccgctg ctgtttccaa ttatgcttta cagcggtccg tcgggggtca atctgtatat
     4501 tatggccagt atcggtgcgg gagttgtgga gcagaaggtt atccgcaaac acctgcaaga
     4561 gcagcaggaa gccgaagagc ggggcaaggt tcctgtaact gccaaaaccg gcggcaaact
     4621 caaaaaaaga aagcccaaac ctttctttaa atggtaaggc actgcgttgg gctgttatcg
     4681 gacagccgcc gtaaagtagt tttaatttgt cggctatgtt gagtatggat gataccattg
     4741 cagctgtcag cagtgcatca ctgccggccg ggacggcagg gcggattatc atccgcatca
     4801 gcggacctca aacctggcca attgtggagc agatccttac accggacaaa ccatttcaaa
     4861 agaaccgaat tgcggattgc cgtgtccata taggggaagg attggatatc cgcggtctgc
     4921 tttatgcatt ttttcagccg cattcctata ccggtcagga tttggcggag ctgcatctgg
     4981 atgtttgcag tgcggctgcc gatgcgatct taaaaaaaat ctattgttca gcgcgtcctg
     5041 caatgcccgg ggagtttacg cagcgggctt acctgaatgg tcgcctcgat ctgacgcagg
     5101 ccgaagccgt ggccgaaatt gtagcggcag ccaacacagc tcagctggaa gcagccgagc
     5161 gtctgctgag cggccgcttt tcggagaccc tatcggattt gcgaaatgag atgcttgagc
     5221 tgctgggtct gttggaagcc gaactggatt tcgcagagga agagatagag tttatctcaa
     5281 aggacagagc ctgtgagcaa atcagccggt ttcagcagcg catatctaat cttctggaag
     5341 gcagcatacg gtgtgagcgg atcattgata tggatgctgt ggggctggcc ggcctgccga
     5401 atgcgggcaa gagccgcctg ctcaatgcac tgctggaaac ggagcgaagc attgtttcgg
     5461 atgcagcagc gaccacgcgt gatgtgctga cgggcattct gcggctggac ggattggatt
     5521 gcattctttt tgattgtgcc ggtctgatgc ctatctccga gcagacagct ttggttgatc
     5581 ggatggcgca tcaggcctct ttaacagcgt taaataaggc agcgttggtg ctgttttgtg
     5641 tggatggcag caaggaggat attgaaaccg atgtacagat gcgcaagcag attcagatgc
     5701 cggctgtcat ctatgtaatg accaagagcg atatcctcag gccccaagat gccgcgcagc
     5761 ggtgtgagca gctgcagcag gtattcggag gcgaatttct tccgaccagt gcggcagcag
     5821 aaagcggatt ggacgcattg aagcagcgca tacgaatgga gctgctgcgg ctgcgggccg
     5881 gcgatcggga acatcaggac cgtttgacga tcaaccagcg ccacgagcaa aaacttcagg
     5941 agacgctcaa gctgcttgcg gcggcggacg atgaaatacg cagggattct attgagactg
     6001 gatccatgct gttgcgtcag agctgtcagc ttctgagcgg actggagcat gaggatataa
     6061 gcgagcagat tctcgaccgt attttttcgc ggttttgtat tggaaaataa agaagcggta
     6121 tggctgaatt aaaaatactg acaggtccac tgagaggcca tgtgtttgga cttgaaaagg
     6181 gcggctgggc caagaccctc ggccggggcg ccgaaaatgt cgatttccgc atacctgatg
     6241 ccgctatctc acgccggcat gccgaatttt cctatcgcga cgggcagtgg gctgtccgcg
     6301 atttgggctc ctccaacggc acatttatca atgaaatccg aattcgtgag acagcgctgc
     6361 tgcagcatga agaccagatt cgctgcggtt caaccgtcct tctgtatagc aatcctgagc
     6421 cggacggcct gcgcgccggc cgatcggacg agaccaatgc aaaacggatt gagctggaat
     6481 atgatccggg tgaaacaatg gtggctgtgg cgtttgattc gctccaatcc aagcagacag
     6541 cgcggcagcg gcggcggatg atggaagcag gacaggcggc gctgaacctt tcgcacggca
     6601 tcaaaaatat tctgcagaca ctgcggtcgg gtcaggatgt gatggatgat gcattggggt
     6661 atcgggatat tgagcaggcc aaaaaggcct ggaatattct taaacgcaat ctcgaaaaag
     6721 ttcagaagct ggtgctggat atgcttaaat tcagccgtga tgaaacaccg catctgcagc
     6781 cctgccagtt caatcgtttg atcgagtctg tgattgaaat ccttcggccg caagcccatg
     6841 aacggcaggt cagcattctg acacaatacg atgaacatct ggaactggtt tgggcagatc
     6901 ccgaacagat gcaggatgtg gtgatgaatc tggtgctgaa tgcggtagaa gctgtgccgc
     6961 cacaaagagg acaggttacg atacgcacgg aactggacgc ggcaggccaa gccattctgc
     7021 agattatcga taatgggccg gggatagagg atacgcgagt catttttgag ccgttttatt
     7081 ctaccaaacc gaatgtcgga gctggtctgg gattgactat tgcccgcaaa atcatacggc
     7141 atcacggcgg ctgcatcgaa gtccaatccc tgcccggcca aggttctatt tttaccgtgc
     7201 gtattccggc cggttcagat aaaaagtaaa atccgcattt tatcagacga tggaggaatc
     7261 ttcaggaatt tcaaagcggg gggattccaa ccttggtcga tccgataagt aaggaagata
     7321 ggttagagca ggcggcaaat cctgataaag tctctctttg atttggccga tgaagggagc
     7381 gatagataca gtgcctatgt atgagaaaaa agtatctgtc ccgggagcaa acaatgaata
     7441 caaaaaatac ccagattaaa atgccggata cctttttcgc tccagccggc cgtgagcagc
     7501 cccatcaagt ccgcaacctg aatgcacgga tagaatcaaa cccatacgta aagtcgatgc
     7561 ttgacgcgtt gccgatgatg gtgctggtgc tcaactccca tcggcagatt gtggctttca
     7621 atgagacggt tttgaaaata acaaagtcct cggcagaagc agtgttagga aaacggccgg
     7681 gcgaagtgat taaatgtgtc catgccggtg aagggccgga cggctgcgga acgagtgcag
     7741 cctgccggtt ctgcggtgct gtccaagctg tcctgcagac gcaggccgac agctgccgcc
     7801 ataaggaaga atgccgtatt cttatggaac agggattggc gctggattgg aaagttacag
     7861 tctgcccctt aatgatggat gggtattctc tggtgtgtgt ggtcatagaa gatatcggcg
     7921 atttgaagcg ccggcaggtg atggagcgga ttttccttca tgatatgatc aatcatctgg
     7981 gggctattgt cggctttaca cggctgctgg ctgaagaaca gggcgagaac gaagaattaa
     8041 aagaggtgat tcagcttgcc gacggattgc tggaagaggt acaagcccag cgcgatttga
     8101 tgctggccga aaactgtgaa ttagaagcca aaagagaacc ggtcgccctc aaatcctttc
     8161 ttgaccggct ggtccagctg tacaggagcc atccggtttc agcaggccgc ctgattgttg
     8221 cggagaatgt tgcggatata agcatagaaa cagatcccag tctgctgaga cgagtggtcg
     8281 ggaatatgat taaaaatgcg ctggaggcat cggaagccgg acagacaata accgtgtcct
     8341 gccggcaggc cgaggccggt gctgccgtca gtgtgcataa tcccgcagtc atgccgccgg
     8401 atgttcagat gcagattttc aaccgctcgt tcagcaccaa gacaggaacc ggccgcggca
     8461 taggcaccta cagtatgaag ctgctgggcg aaacctatct gcatgggcag gtcacctttg
     8521 aaagcagccc ggaggccgga actacattta ctctgaccct gccgctcaaa agcgtttaag
     8581 aggcgatttt ttcggcttaa tcaaaggcag cgggaatcgc aggattgcga aactgggcaa
     8641 aaaagagttt aagttcgtga tgcagggtcc gtccgcggga taaattggcc ggcaaatctt
     8701 cttctgcaaa ccagccaatc tcacttgttt catgactgac agtcggttgc ccgccggtca
     8761 gttcgcacag gaaaaagagt ttataaatat taaatggata aggcggggta tgtccctgcc
     8821 ggtcgcggtc aaaaacagca agcaggcgga caggcttcac ttcatagccg gattcttccc
     8881 agacctcgcg agccacattc tgggcggctg tcattccgca gtcgcaccag ccgccgggca
     8941 gagtccatcc tccgtcggca gcctcgcgta ccatcaagat tttatcatcc ttgaatacaa
     9001 cgcctctgca atccactttg ggagttggat agccggcatc ctgctgcaga atgccccgcg
     9061 cctgttcggc attcagttca gtgtagtgag caaaaatctc ggcacaaatc tgctcgagtt
     9121 cctgatggcg attgagttca tactcgtttt gggcaaagtg ttttccggcc tgtgaaatgg
     9181 ctttcagccg tttggctatc tcaaaccagt caaatgcatt cgaattcatc tgtgttcccc
     9241 tgtttgctca aaaagttcca tcacggccgc ctattataat cctgctgcca agcaaaccaa
     9301 agcgtcattt tctttatcag gcgggaaatt gtcccttctt tttcaatgct tttttctctg
     9361 ggatgcgata tagttttgct gaatcgcgtt ttataagaaa atgactgcgg atagtgttcg
     9421 gggcggctgg aaaaaaaaga aattttggag gcacaaaatg ctaaaaaggc aggtgcattt
     9481 agaggttatc cttttagctg gagtatttat atcaatggcc tcggctgaac ggattgtacg
     9541 ccggctggat tttgggtgga agttcatcaa aggtgatccg gcaggggcac agggaccggc
     9601 atacgatgac agcggctggc gggatgtgca gattcctcat gattggagca tagaagggcc
     9661 gtatgatgcc aattgggcca gcgctaccgc ctacctgccg gcaggaacag ggtggtaccg
     9721 caagacattc gaattggata aaaccctgca cggcaagttc atatctattg aatttgacgg
     9781 tgtttataac aatgcagaag tgtggattaa cgggcatttc ttgggccagc gtcccttcgg
     9841 ctatatcgga tttcaatatg acctgacgcc ctatgtcctt tttggtcagg agcagaagaa
     9901 tgtgttggcc gtgcgcgttg atcacagtca atttgcagat tcccgctggt acacgggttc
     9961 ggggatttac cggtatgttc gactatgcat caccgatcca gtgcgtattg cgcactgggg
    10021 aacatttgtt tcggtgccaa aggcagatcc ccaaaaggcc gagattcaaa ttgaaacaac
    10081 tgtcgagaat agaggcgggc aaaggcagac tattaccctg ctttctgaaa ttattgcacc
    10141 cgatggcaaa ctggcagcaa gtgttgtcag cagcaggacg attgaggccg gcaaaacatc
    10201 tatttttgaa caaacagcag cggtgagcaa tcctgccctg tgggatgtgg acaatccgaa
    10261 cctttatacc ttaaaagctg cactctccat agacggcaag ccggctgata ccttagaaac
    10321 gccttttggt gttcgctggt ttcggtttga tcccaatgag ggtttttttc tcaatggcag
    10381 aaatctgaaa ctcaaaggcg tttgtctgca tcatgatgca gggccggtag gagctgctgt
    10441 tcctgaaaag atgtgggttc gccgactgaa ggcgctcaaa gaaatcggct gcaacgctat
    10501 ccgctgcagc cataatccgc cggcaccgga gtttctggac ttgtgcgacc gcatgggctt
    10561 tctggtgatg gatgaagcgt ttgatgagtt taccccgacc aaaaacaagt gggtcaaagg
    10621 ccgcaatgta ggtgttcctt cccgggacgg ttacggtcag gtttttgaaa aatggtctat
    10681 tcgcgatatt caggatatgg ttcgccgcga ccgcaatcat ccgagcataa ttatgtggag
    10741 tatcggcaat gaaatagact atccgaatga tccattttca catcctgtgt tgggtgataa
    10801 ttaccggccg aataatccgc cggcacggga tttggtgcga tggggcaagc cgctggttga
    10861 tgcggtcaag caattggata ctgcccggcc tgtcacggcg gctattgcaa gtgcaccgat
    10921 gtcaaatgcg gctggttttg cggatattct tgatgttgtc ggctacaatt atcaagaaca
    10981 gtactatcaa tcccatcatg ctgaatatcc ccagcggatt ctttacggca gtgaaaacag
    11041 cacctcgctg actgcctggc aggctgtaga ggacaatgat tttattgccg ggcagttctt
    11101 atggattgga attgattatc tgggtgaatc acccggttgg cctgtaagaa gctggaccgg
    11161 ggggttattt gatctgtgcg gcttcaaaaa accgctgggg tggtttcgtc agagcttatg
    11221 gtccgccgag ccgatggtat atctggtctg cagcccagcc agacgccaga cgggacaatt
    11281 tgaccgacgg ggcgggcgga tgtttcagtc acactggaat tggccggaag ggcagactgt
    11341 gcgggtggcc tgttatacca actgtgacca agtcgaactt tacctcaacg atacctctct
    11401 gggcgtcaaa aactttgctg atgcggtgcg aaggacgctt gaatgggaag tgccgtatga
    11461 aaaagggcag ctgaaggctg ttgccagaag ggatggaaaa gcagtttgcc aatatgtgct
    11521 ccaaactgcc gataagcccc agaaaatcca attgattgcg gatgcagccg aactgaaagc
    11581 cgatggacgg gatatttgtc atctggaatt tcttgtgaca gacaaaaacg gcgtccgtat
    11641 ctacgatgcg gccaatcaaa tcacattcaa aataaccggc cccggcaaaa tcatcggtct
    11701 gggtaatggc aatccggccg cacacgaatc gcatcaggga aaagcgtatc cggtattcga
    11761 gggcaggggg ctggcaatca ttcaggcagg tcaatcaccc ggtacaataa caatcgaagc
    11821 atctgccgag tccctcgaac cgacaaagat tactcttgat gttcgttgaa cattaaaagt
    11881 ttttgatgcc ggtaaagacg atttgagcgg ccagcgccgt caggacaaga gcagtcagct
    11941 tggtcatcac ggcaatcact ttttgtccga agatattgcg gagctggtca gccataagca
    12001 gaaaaaggga catagtcaca gcagcggcta agatgccggc aaacataaca agccgctcat
    12061 ggggatttgt aatttgggtt ccccagacca tcagtgtgcc gatagtaccg ggacctacga
    12121 taatcggcag ccccagcgga acaaccgcaa aatcttcgcc ttccgtatgc tgcgggggct
    12181 tgctgatgcc cagtaccagc attaccgaag tcaaaaacag agttgttcct gtgcctatct
    12241 gaaaggccgc cagcgtaatt cccagtaggc cgaacagggg ttttccgaaa aagaaaaaga
    12301 ccgttactgc aatcagaaca gttgcagagg cccgcattgc agcccgccgg cggcttgatg
    12361 catccatttc ctcggccatt tttaaaaata gtgacatcgt aaaaaacggc gccagcagaa
    12421 aaaacatctt aatataggta tttacaaaca gcgaaagcat acagggcctt ttgttatcga
    12481 attttcccgt atttatcctc gaaagcgccg attatgtcaa caaattcctg cggaagccgc
    12541 agcagggctt ggtcgattat ccattgggga attttctgat aaaacgcctg tgcaatgccg
    12601 ccggcaatac aggccagcgt gtcgctgtcg ccgccgagag aaacagccag gtggatagta
    12661 tgttcaaaat ccgtcgaatc aaaaaatgcc gtaagggctt ccggcacggt tttttgacag
    12721 gacacattaa attggtatgt cggccgaatt tgttcgattg tgcgattcag gtcatatcca
    12781 aagcggcggc ggatttcctg tcggatagag tccttatcac agccgcagcg agccagataa
    12841 atcgccaaag ccgttgcctg agctcccttg atgccttcgg gatgattatg ggttggctcg
    12901 gcgctgcgcc gggcttcttt taagacggcc tcaaccgtgt caaacgcaaa gccgaccgga
    12961 ctgacccgca ttgcggagcc gttgccccag ctgttatacg gtcctgcatt gtccgacatc
    13021 agccagagcc ggaaattgct gccgtagccg cgctgcgggt agcgcctgcc gaaatcccga
    13081 tagacagcgg cgtaatccaa gccgtacaaa atcgccttcg cagccgctgc agtcattact
    13141 gtatcatctg tgaaggtgca ggcctcttgg aacaaaggaa aatcggcagt cttaatctgt
    13201 ttccattcgt acacagaacc gacgatatca ccgattattg ctccgagcat cgctgcgacc
    13261 ccaataaaga cgaaccttgt tggctgcctt gccttagcag cggtcaattt cctttactgc
    13321 cgctgcatgc gtgtccaatc ttcgcttcgg acgccttgat aataggtaac cacatactca
    13381 atcgtttttt ccgtttgctg gtctaaggtg agagtgtatt ttattgtatc cagatcgact
    13441 ttttcataat aggtgtcagc atcgccgaga tttttaatgg tccagtaaga tgtagggaaa
    13501 ttgcgcttca gctcaagttt aaccggcaga gggcgtgtat ttttcagttc gagcttgaat
    13561 gtgcggattt cgtcccagcc gttcacgttg ccgtctttgt caaatcgata attatccgtt
    13621 ttttcgtcca tcagtatcgg attgacaata acatttgaaa caggacccag attcagttca
    13681 acatcttcac cgacaggaat atatttgaag ctggattggc cttcatagga caaatgttgc
    13741 tggtcgtcca cactgcggaa gactttcaga ctgccgccgg ggataggggt ttgacccaac
    13801 ttgtgctctt ggtcgttttt gaagcttaaa tagcggacaa cctgcggtcc ccagcggtct
    13861 tcctcgaatt tgtacaggtt gacaacagga atttggtcgg cctgaaaact gggcaggcgc
    13921 ttggaccact ggtcaggaat ggtttctgtg ccttcgatag tatacaggaa atattcgctg
    13981 agaccctcct tgcggatttc tttggtctgc agtgcggcag catctaatgc gagcaattct
    14041 ctgcgtgccc tccccgccaa agcccgcggt tcttctattg ccgaagcagg ctttgcaggg
    14101 aagacttctc ccggacggcc gtaaggatat tgccggcgtg ccaattccgc tatctggtcg
    14161 aggatatgaa cctgccccac aatgagacga gtttgggcgt tttcataatc ttcgcccgaa
    14221 ttattggaaa ccaccacata gccctccaaa tgcatggttt tttcattgtt ggacaatgtt
    14281 cccagataga acgctcgcca cgacaagccg ctggtcagat aagtgatttc gactggtacc
    14341 cggccgtcga tttcgctttt gatgttccat aatccgagat tctgcacccg cgggggatat
    14401 gtcaggtctg caatgtcgat tttatcggcg taagcctgag gctgcatcag caggctggtc
    14461 ggatcaatca atgtattggc ccacgagaac tgaagtttat tatcaccttc tttgagcgtc
    14521 agcgagcggg catcccgcac cagtgtcaga tcggcagaat tgtaaattgt cagctgcgtc
    14581 atttctttag ccggcagggt tacgaggtca accttggcag agacattgcc cgccagcagt
    14641 gcagcgatca gtatcaggtt tgtcattttc ataatcaatc tccttgtcga tatcagagca
    14701 aatatgcttc tctctatttg cccaatgttt tttgcagctc gaagtgcatc atattatttt
    14761 tacggacgga cattgattcg gctgtagcgc atctccaatt ccttgactgc cggaccgtct
    14821 tgggtacgag caggcagctg gatttcaaac tccagcgtcg aggcatcttt gcgggtgtat
    14881 tccatattgc agttcttcat ttcccactgc cccggaatgt gctgtatcat cgtcagcacg
    14941 gccggcttgt ctttgaaatt ctcaattttg gcggtgataa cttcgtcggt gtcataaagc
    15001 acaatggctc cggcattgtt tttgcggagg ttgatgcggt cgtttttcat ctgccgctgg
    15061 gtgactacga tatcgcggct gtcgccgata tagacttctg ttttctggcc gaccggcacg
    15121 atagaagtga agtcctcgcc cagaaagatg gtgctttcgt gtccatcatc ctgaaatagc
    15181 cgcatcttac cgccccagag cgaaaagtcg cccagcccgc tttttttgtc attgacgatg
    15241 cgataactga cagggatgcc gatgttgcgg ttttcaacct gttccggatc ccagggcagc
    15301 ttggctgcgt cccagaccca aatcttggtg atgggtactt cgggggcttt gagaaacaaa
    15361 agctgcttgg tttcctcatg ggcaatggac tgctcgaaac tgtcgccgta gtccagcaat
    15421 acggcggcct tgtcaaaatc ctcgccggaa aagttccgca gaatcagata gctcttgaga
    15481 tcaagcttgg tttcatcttt gtcggcgacg gctttgtagg ttaccaagcg atcgatattg
    15541 gccagcagat agctgatgcg gaccgtttca gcataatcgc cctcggcggc aatctcccaa
    15601 accagagccg cctcgttggg cggatagctg acattgagca gcttaacctg ttccggatgg
    15661 tccagaatgg tcagccgaat cgaatccgga tcgatgctga cagcattcca cgagaagtcc
    15721 actttgttta gtcccttctg aagcgtcaga acgcgctctt cctcaatcag tgtggcagcc
    15781 ggattatcca gccgaataac ggttgcatcc cgctgcggca gggcgaccag tttggtccgc
    15841 ccgtgtgcgg cagctgcaac agccagaacg gcgatgctgg ttaaaatggt ttttgctttc
    15901 atagtactgc tcctttcaca aaatagcgtt attgttcttg gacaggattc ataatccggc
    15961 cgatgaataa aatactgccg gttttattat cttttatgac aaatacaaac ggccgatcgg
    16021 ctcggaatac cagcggcggt tcaggtacag cggctgtggg ggcaatcacg gctgtggcgg
    16081 cggctgcctc ggtgccttct tcatttactt ctacgaatgc tttatgaagg acatcggata
    16141 taaaaaggtc ttttgtgccg gttatccctg aaaagtctgc tgtgctgcca aaggcgtccg
    16201 gcatgcccat tgctgccagc gtttgagaca gcgaaaactg cgactctatc ttcaatcgcg
    16261 gcaggtaaac gtcaaccacc ttcatttgca tgctgttcat ccattgggtc agtagttttg
    16321 ctgtaagatc ctcttcaaag cgtcctgcat atcctttttc cggcagcagc acgaccatgc
    16381 tcaagtcgcc gcctttgtac ggcagttcga gcgtctctac cttaatgccg cgggcagttc
    16441 ggaagctgac gtgttctcca tatctgtatt tgccgcggcg gtgcatcagc ggcgccggaa
    16501 ccgtcctgtc atgcgaaata taaaaaggct cattttgggt cagttttgga tcaaactctt
    16561 cagcccagct ccctttaaaa taaatggcgt tggtcagcac cagccgcgtt gccgcactca
    16621 gggcgccggc gggaatcagg tttttgattt tgtcctgagt tttgtcctga atccattgat
    16681 tgattttcag ccgcacggct tctgtttcgc tgataaaatt cacagtttcc agtccggcat
    16741 ggtaaaaatt cttattgagc atgatgaaag aatccaaaaa tggataatcc ttctgccccc
    16801 acagtgcgtt ggcgatggaa agcagatagt cgcctttctg tccctgccgg ttcatctggg
    16861 ttatcagccg gccgaatgcc ggatgaaagg gctcggacag ctccagagca ttgggttctg
    16921 tatcgaaggg aaaatgcagc gtctgggcca tttgccgagc agtttgctct tttgcgcctg
    16981 catagacaag tgccagcgct gtcgaaatgc tgaaaggaga gaaaaacaga ttgccatcct
    17041 gatttttcaa ctgtgcgtaa agttccaaag caaaggcggt attgccctca acaatggctt
    17101 tgctgtatgg ttggaattca gcccaacaaa tgcctgcggc catcagcagc actgcggccg
    17161 gcatgaacag tgctttcggt ttcatttttc ctgctccttg ctttgtgcag tcattttttc
    17221 aacataatat tcgcttcgcc gctgctgata tttggtgacg gtatagccaa atgttttttc
    17281 cgagcgcggg gcaagcacca gtgtaaatcg ggcatggctt ttgtcgtgct ttgtataagc
    17341 agcatgcggt gtggaattac cattggcgtt tatgcccgat tcccagtaat cgtctatttt
    17401 caattcccaa tagtcggttc cgaagtttcg agttatctca atctctgcag gaatctggcg
    17461 ggtattggtc agtttcaatt gccacgattc gatttcatcc cagccgtcga tatctccatt
    17521 gttgttgaat gtatagttgt ctgtccggct gtctatttgt atcggctcta ccttgaccag
    17581 cagcgccgca ccgaggttca gttccgcatc ttcattgaca gggatatact ttacctcagc
    17641 agacccgata tatgataaat tctttttttc attaagtcgg cggtatagtc gaacattgcc
    17701 ttccggcagg ggagtattgc ccagtttgtg ggctgcatca tttttgaacg agataaatcg
    17761 gatggtatct gtcccgtacc ggtcttcgtc atatttgtac aggcttttga ccggtatgcc
    17821 gctggcatcc agtgattcca atcgcttggc ccatgcattc ggcaagtctt cagtgccttc
    17881 aatagtatag agataatatt cgctaagagc ctccttttgg atatccttga tttcgtattt
    17941 gaatgctccg ccgccgaatc ttccgctcgt atcagaaaaa agcatgcctg tctgagcgaa
    18001 atcagccagc aaagggactt ttttgagagc atctttataa atatattttt catcctgctg
    18061 aggaacatct ggtccatacg ggtaataccg accggccaga taattgatgg tttcagtcag
    18121 tcgtgtctgg ccgacgacca gccgcgtctg ggcattctgg aaatcttggc cggagctgtt
    18181 agcgacattt acaaatcctt tcaagtccaa tgttgtctca tcttcgttta gcgttccttc
    18241 atagaatgcc cgccaagaca acccgctgac aaaataggta atctcaaacg acaccgcacc
    18301 ctcaacttcc gaacgaatga gccagcgtcc aatgtctttc aaacgggccg ggtaaacaag
    18361 ctgctgagta tcgattttgt cggcatactc cagcggctga agcgataaac ttgtcggatc
    18421 aatcaaggtt tcagcccaca taaactgaag ccagttccag ccgggcttca atgtcagacg
    18481 ccggatatcc cgaaccagcg tcaaatcggc ggtgttatat attgtaacct gcattgtgtc
    18541 aggggcaggt aaaacaacca gttctatggc gctggcgggg gcaaatggcg gactgtctgg
    18601 tatagaatca gatgatacaa ttttaacact gttttgcact ggctgtgcac catcttggat
    18661 gaattttgtg taagagaggt aatctctgct gtctttaaat tgttcccttt tttttggacc
    18721 gaaacggtcg gctgcaccga ttttgttttg gctgtcaggc agaaatatcc ataatgctgt
    18781 aaccactaaa gcagctgcgg ccgcccagcc aatccatacc ttacggtaaa aggcacctat
    18841 tttttctcca gccattttag acataagtgt tttagaaaca agatcatcgg gcagttcaac
    18901 atcaccttca agaacagaca ttacagagaa ttttcttttt aattctgaca aatgctgtcg
    18961 gcattcgggg catttgtcta aatgctgacg aactgctaag ccggcgtttg cttcaagaag
    19021 cccaaaggca tattgaataa gaatgtcttg attgaagtga ttcattgatt ctctccggct
    19081 gggtcaggca gcagggcagc catttttttg agggcacgaa acaaatgtgt cttaactgtt
    19141 ccgaccgaac agttcaagac ctcggcgatc tgctgatgag tgaactggtg gtaataattc
    19201 aggataaggg ctgtccgctg ctgttctggc agcttgccga gagcatcgcg tacctgtcgg
    19261 cgctgctcat cgagtataag ctggtcagcg ggttgtggag ctccggccgg caaggttgtt
    19321 tcaagcgtac cgcaatgaat accgtctgca catatcggct ggctgagaga aacggcggta
    19381 tgcttctttt cctgctgtct gcgggtgatg gctgtacggg cagcaatggt taccagccac
    19441 ggccgcaaat tatccccgtg aaatgcagcc gcatatttgt gcgctttttg gaaggtctcc
    19501 tgaaaaaggt cctcagcttg ctcgcgattg tgtgtcattt ttgtcaaata ccccagcacc
    19561 gccggtccat gccggcgaac gattgtttca aacgcccccg tctggccgct ccgaaaggcg
    19621 tctaacagat ttctgtcgct gtcgtccata taggaatcct gctgaggata tagacaatca
    19681 tactacccca aaggttgaca ggaaaaggta aaattactgt aaaaactaaa acccctgcat
    19741 cgatgcaccg gaaagaaaaa aagctttttt aaaaaaatat taaggcattt tggttcgatt
    19801 ttatgctata atttttacta acagaggact cttataaaac aggacttatc aagaacagga
    19861 ggtatattat gaagggtatg tttctatctg ctgctgcaag tatttttgca ttcgtctctt
    19921 acgcaagctg gcaaaatccg gtctttcttc aggaactcaa tgattttcaa aatggatatg
    19981 cggccaatcg gccgatggtt tctagtgatg ggatgctgct ttattttgtc cgcagtacac
    20041 cgacaaagat cgatgccctc tgggaagccc gccgtaatcc cgaaacagga gtattcgaac
    20101 atatgcgtca actttcagag ttaaaatatg gaggcgcaca aatctatggg acgtggcttt
    20161 cgccggataa cctccgcctc tattatgccg cctctgatcc agttgctctg gggtggggca
    20221 agcgccccat ctggctggcc actcgaacca gtatgaacgc tccctggcaa ttggttaggc
    20281 ggttcactga attggaaatc tttaattttc aaaccagctg ctcattaact gctgatgaaa
    20341 aagtgattat gtgggaaagt gttccctttg ctgagggggg tcttaaaaga attttcgttg
    20401 gcatgcgtcc gtctattctg catagttttt cgaatttcag ggaagcaatc gaactggagg
    20461 atattaaagc atacgttcct cgcatgtccc ctgacgggct ttctgtttat tttaaaaaaa
    20521 ttgaacagtg aagggattat tgaagcttgg cttgggcatc gtggttcact ggatgcgcct
    20581 ttcggtaatt ttgagccctt ggaggaaata aataagacag ggataggcgc tgatgtgtgt
    20641 ccggcttgga atggtcagac acttttttat tttcagaggc agggtgaagc tggggacttg
    20701 aatgcaacag gtatttttgt atcgcactgg gttgatgatt catataccga tgcccttgtc
    20761 gttcttggcc gggcagtgat gaaaaaaaga aagggctatt gatctattga cccaagccgg
    20821 tacagacgaa cggaaagctt tacaaattct aagcatcctt cccgcttcgg acctgcctgc
    20881 agaacttgca agcaaaagcc gctgggatgc cgtcaaacat atccaccaat ctatcaaaaa
    20941 gcaggagcag gtggtcggcg tgcttgaaac ctgtatcaca aatctgcaaa ccgcactgtg
    21001 gcttctttct ccgttatcga ttccataata tagagtattt ttaaaacaaa taaaagcaga
    21061 tttagtttca ttttttattt gaaaattatc gcgaagtctt tttttgaatc cagatggaac
    21121 ttcccccgcc aacagaaccg gtgcattcct gataaaactc cgggaaacat ctggccagat
    21181 aattgttaaa aagaataatg tcaaaccgat tattatattg aagaaaagcc ctcagtaaat
    21241 atgcctcgtt ccaagcgcgt gcctcattaa tccactcgat tggatattcg aatggccaat
    21301 aaatatcatg gaagtgaacg atgacgccat cgttaagaac cggcagaata ttaaagaaaa
    21361 tatgattcac atcactgccg atcttagcta catgcgaaga gtcgatgaac agaatatcgt
    21421 tttcctgcaa ttgttcaaaa caagatgatg ggatattctg tgcctgtgtc tcataaacag
    21481 tacaatgtgc cttatcctca tctttgagca ggccgtacag gcggtcgggg cagggttcga
    21541 taaatgtaag ttttatctgc cggttcataa aacggtcatt gatgtccagc ataagagctg
    21601 atgtatgccc ggagccgatt tcgataatcc gttttggtcc gaagcggcgg atgatgccaa
    21661 aaagaataag tgcgccgcct ttgccgtaca ttggattatg gcaataatac cggactttat
    21721 ctgacggatc atcgggaaaa tcaaactcgg caaagtagac aaataaatct tttaacaatt
    21781 tctgctgttc aatcgttcta agttcgattc ctgccgcctg ttttacatcc ctcgggtaaa
    21841 gaatcttttg atgggcgtta acccacgaaa tatccggcaa aggtgaatag taatgcccgg
    21901 gcgggtaatt cacaaaaagt ttacgataat gcctgtgcca atacaaaaag ctggacagga
    21961 ttccaattgg gggaattttt ttcaatttat ctttcaacat gcaataatcc ctgttgtatt
    22021 ttttcaatat atcttgattt tgtttacaaa aactgctgaa atccctttcg aatgagcatc
    22081 acagcgatag cggccagcag cagactgaag actttggaaa cggccttggt gcctgcttcg
    22141 ccgagggcct tcagcaagat atctgaagca gcaaaaacaa ttccggccag caggatattg
    22201 gcaatcacag aaatcagcgt agcggcaatg ccgtattcgg agataatcag caaagaggtc
    22261 gtcagaacag ccggaccggc aatcaaaggg gtgcccagcg gcacggcccc cagtggttca
    22321 ccgggattgc ggcgtgcctt tacaggattc agaatatccg taatcgccag acagaacaaa
    22381 attgccccgc cggctgccat aaaatcgccg atagtgatgc ccagcagatg aaaaacgcct
    22441 tttccgacac agatgaatcc tacagcaacc gccgcggcgg tcagcatcga ctggatgata
    22501 attcgccgtt tttccagccg gctcagacct tcagtcagcg agacgaatat cggcagcact
    22561 ccgatgggat ccaccgctac aaacattgga atcaatgcca gcagaatatc tttgagcatt
    22621 tttttctcct aaaactcctt ttaagcagct caattctaac tcgcccaatt cccgattgca
    22681 attggattgt ttttgcactt gttagatgaa aaaagtatcg tactgcattt cagtatgtaa
    22741 tttttagaca aaagcatatt ctgtcgataa aatagtattg aaccgttctg caggaggcat
    22801 tctatggcca gacgaatagc agctatagct tttatttatg cttgtacggc ggcagcgtgg
    22861 atgatactgt cagttacaat gctggttcgt acggaaagtc tggatactcg cctgcacgaa
    22921 gctgtcggcc agttgtgggg aacggctcat actcaaacgc agccgcaggt atattataaa
    22981 atcccatcct cttcaagcct gagcgaatcc aatgagcaga acgagactgt agaacatctt
    23041 gctttggaca gcagtcaaat agcggttgat ttgaaactgg actacagacg caaaggactg
    23101 ctgtggtatt cagtgtatca ggtgcaattc gccggccaat accgaattgc caatcccggc
    23161 agtcagcagc gtgagattta tgtggatttt gcttttccgt ctgaacaggc gatttatgac
    23221 aatttccgtt ttgctgcgga tggacaggat attaccaatt cagcattcaa gagggccgct
    23281 ttgtccatgc ttttcagatg gaccccggcc aaacgaagga aatagaaatt gcctatacat
    23341 cgcagggtat ggatgagtgg atttatgatt tcgggggcgg tgtcaagcag gtgcggaatt
    23401 ttgtgtttgt gatgacgaca gattttgacc ggattgattt tccgcagagg agtgtttcgc
    23461 cgacagtcaa ggccaagact gcaaaagggt ggaaactggt atgggcctac aacaatatgc
    23521 tgacaggcgc ctgcataggg atgacaatgc ccaaaaaact caatccggga ccgtgggtca
    23581 gccggctgac ggctgccgcc cctgtttcgc tgtttctgtt tttctttctg ctgtttatct
    23641 gcacaaccct gcgagaaatt aaaatccatc ctatgaatta ctttttcctc ggcgcagctt
    23701 ttttcagttt tcatctgctg ctggcctatc tggtcgattt catttctatt cacattgctt
    23761 tctggatctg ttcggctgtt tcgattgtgc tggtagtttc ctatatgcgg ctggtggccg
    23821 gaccgcgatt tgcctttctg gaagttgccc ttttccagct tgtgtatctg gttgtctttt
    23881 cttacacatt tttctttgag ggctataccg gactggcaat cacggtactg tgtatctgta
    23941 cgctgtttgc agtgatgcag atgaccggcc ggcttgactg ggaaaaggtc tttgctaacg
    24001 gcctcccaaa gccggctaag tcccgcaaac cgcaggacgg ggggcaaaac cgccccaatg
    24061 taccggacta aaaccggcag ggtattattg gacaccaagc cttgttttgc ttggcagatg
    24121 aaatgcaatg cacaacggca atgatttcag tataaattat tttcctcgag taaaaatctg
    24181 cttgcagata cttgttttta aaatataaag tatcataata ctgccatgca tttggttata
    24241 gtataatgaa caaaaggtat ttttgatgaa taaatccatt aaaatacttg ttgtagacga
    24301 tgatgctgta gcccgaggcg ttttgataca ttatctttcc agctacggaa gctgcacaga
    24361 ggcggaaaat ggcgcaaaag ccgtcgaaca gattcgtcag gcgattatag ataaggcacc
    24421 ctttgaagtt gtctgtctgg acattatgat gccaaaaatg gacggattag ataccctgca
    24481 ggccattcgc caaattgaaa aggaaaacca gatcgaacct gccaatcagg ctaaagtgat
    24541 tatgaccacc acggccagtc aggtgtccaa aacaatgaag gcctttcatt acggctgcaa
    24601 cgcttatcta gtcaagcctg tttccaaaga tgacctttac cgcgaaatga tgaagctacc
    24661 tgtcagcgaa cggtataagg cctactggcg aaccctgtaa tcggctcctg taggcattat
    24721 ttgaccttca ttatccttac gccgtaaaga ccgcctgccg tattattcgg ttgggcttga
    24781 aagcgaatgc ggacgcttgt tttatctctg gtcagttcag aaggtaccgg ataggttacg
    24841 tcaaagaatt catttggttt ctgattattc agcgattggg aggcaatttt tgtcccgtcc
    24901 acaaggatat caaacgttct tgcgccggca tccgaacccc agtaagtaca gaccagttcc
    24961 atcggcacat caggcagtac tttcattgta aattcgaacc agccgttata ggcatgccgc
    25021 catttgcgtc ctgcgaattc gccggcctga gtgttttcac ctgtcaggtt atggtcgcgt
    25081 tcgggctgca tttctccgat gcgcagcaca tcaaccgacc gcgcttccag ttcctgcaat
    25141 cgctgctgtt cggcagcgta ttcagttttt ttctgctgcc attgttcggg tgtaaactga
    25201 tccatataaa ccgtataggg ctggtcagcc acagcataca gcggcagcag ccgcagttcg
    25261 cgcatcgccc cttcatcttc aatatcccgt gcaataccgg cagcagtaaa ctccagcggc
    25321 cggccctcaa caggtttgaa ggcagccagc aattccgccg gcgttccgac aagcagcggc
    25381 aggggagcat cgccgctcaa atccgccgcc agcacaatcg gaccatacag aaatgcgata
    25441 cggttgggct tgtcgggcat cgcctcagtc cgcagcgtca tcggcaattt taattcgaca
    25501 acatccccgc ggttaaaccc tcgattgacg gcaatatagc cggtgctgtc cggctcggtc
    25561 gagacgggct tgccgttaac cgaaacagtc atcccggtag tccaagcggg cttgcgcagc
    25621 ttcaaaataa atgtggtggt tttaggacaa tcgattgtca gcttcaccgt atcacgggcg
    25681 gggaaagccg tttcctgcgt cagcgtaatg cccttgtcct tccatgtcag caccgacggg
    25741 ataaacagat tgacatacaa tgcgtttccg ttgtggaaat aaatcgtctc gccgtatttg
    25801 gcgtggtttt caaaacctgt gccgacacag caccagaacg agtcgaacgg atcgcagaaa
    25861 tgcttgcggg cgggcatgtc cagaaagccc ttatacataa tcatccctgt acgcggatgc
    25921 tgatgtgcca gaatgtggtt gtatagggca cgctcgtagt agtccatttc ggcagcgcgg
    25981 ggctgtgcgg caaacaaatg ccgcgtcagc ttgagcatat tgtaggtatt gcacgtctcg
    26041 gtggtgtcat gcatcggttc ggacagcttg tccggctgac cgaagtgctc attggcgctg
    26101 ttgccgccgt tgacgaaggt atagtggctg accacagtgt cccagaaaaa gcgggcgatg
    26161 gtcttgaaat tctccatacc cgtcatctca tagatgcggg cggcgccgat aattttcggg
    26221 acctgtgtat tggcatggcg gcccgccaga gaatcctttt gattggccag cggatccaat
    26281 acctctttat gatagaactt cagagcaagg attttatact tttcttcgcc ggtcatagca
    26341 tacaggtcga taagcacttc attcatcccg ccgtgttcac aggccagcat tttctgccat
    26401 tgctcatcgg agaggttttt ggttacctcg aatgtccaat cgcccatttt tttcagcact
    26461 tccagcgcct tggcattgcc gcagtaaaca aacgcatcgc gcaggccggc cattactttg
    26521 tggttggtgt accacggtac ccagccgccg ttgaggtcga agccggccga gcggatatcg
    26581 ccccgggcga tttcttcaaa aatgcgcttg ccgttaggat acgccgccag atagccgttg
    26641 ccgttggctt cctgacagag ggccagttcg tcgataatat agtcggcacg ggctttgtac
    26701 tgagcatcgc cgctggcagc atacatcatt gcacaggccg acaaataatg acccagacaa
    26761 tgccccgcaa ctccttgact ttcccagccg ccgtagactt tgcccttcgg ctccaaaccg
    26821 gcttctttac ggaaccacgc cagaaagcga tccggctcca gtttcagaag ccattgagcg
    26881 ttacggttca ttgcatcctt aaaaatacct tcggtcagat ggacgtccga aagtccaaac
    26941 gcataaaccg aagggggcga aaccggctgg acggcatacg gatatggcag agccagcgaa
    27001 tccccggaag caattgaggt caaaaagcag catactgctg cagcagttac cagagttttc
    27061 atactgtgaa atttcccttg tgttaaaatt gaagtacagt ttcgttcatg tcttccgcaa
    27121 catgataaaa taataagaac tttgtagtat ccctgttttt gagccaaatg tcaagggttc
    27181 gactctaagt gtaatccgcc ggcaatatag tgcggtagga aatatttgag gaatttaggc
    27241 aatgcagaag atcctaaaac ttatatcaag gaaaatcagg tctgttatag catcttttgc
    27301 ttataaggtt tatgtgtcgg atgtaagtat ctgttttgat aagaacataa gagcttagcc
    27361 ggcaatggtg caagggatat ttaatcttcc gcaagagtat gttttttctt aattttcata
    27421 tttttttgtt ttttgttatg aaagtgagat aaaataataa gttacttaga gagactgctt
    27481 gcagacaaaa agcatccttt atactgcagc ggcagttcga tgaaaaaagg agaaagagtt
    27541 tgtttcattc ggaaaatcaa ttttccggaa attaccgtca attgctgtct ttacccattg
    27601 cgcatcgtca gtactttccc atcctgattt gcggataaaa acatgccggc agaatggctg
    27661 caggcatgtg acaagattga tttgatttac ggagggttaa acatgaaaac acatctattt
    27721 tcttatctta tcttagttat gcttcttgtg ttgtgttgtg cttctgtaac tgcctacgaa
    27781 gtttcggccg ccggtgcctg cagcagtccg aattcgcatc ccgatgccaa gggcttttat
    27841 gacaaagtac ggagcttttc cggctggacc ggcaattttt atgtagagga caacaacagc
    27901 aaagaaatac attacaagcg ggtggatatg ggcggccaaa acaacagctg ggtagacagc
    27961 agcgatatcc attaccatgt cagtcacggc ggaacgcgct gggattccta ctacggcaaa
    28021 gatctaaagg ctgtgctgtt tgaagacggc accagtctgg ttcccagcga agcccgcacc
    28081 tgctggggcg attcggacct cgaatggata gcatttcgca actgccagct gctcaacgat
    28141 gtttcccgcc cctactgggc caatgcgatg aacgggctgc atctaattct cggctttgtc
    28201 accaacagct ccaagcacga caatttcggc aaactatggg cagagaaaat gaaaaagact
    28261 acgattttgt ggtggaccat ccccggtcag acgattacac aggcttggtt taacacggca
    28321 gatgccaccc agcccagcgg ccgcattgct cgcgtgctgg ccgaagtgca ggacaactac
    28381 aacgaccacc tgtggggcaa cggctatgtt tcacctgatc ctccggtgga taattattac
    28441 tggtattggg accataccgc cggtagtccg ccctatctga atgtcaatat gctggagatg
    28501 atgtacatct acaaggttat gccgcgtact gtgaatgaag agtatgtgca gcagattgga
    28561 aatgcctttg gcctgcaggg tccgattggt cagaactgcg agagctattt tatggcagac
    28621 ctttcaaatc cggccggtcc ccgtattctt gaaatatcca agaccacagg ccatttccat
    28681 ttccacgatg acggcaagct tttcgttctc gatccgcagg gcggcaaatt cccgccggaa
    28741 caggctcttg aagtggctca ggcatttttg tcccgcaaca aactgttgcc gcaggatgct
    28801 cgggcttatg cggtagaata tgatacgctg acagaggaaa attatgaaaa aggcaccatc
    28861 cgacagaggc tgacgatgaa taccaatgtt gtctgggcac gccagattcc tgcggatccg
    28921 aagcagacgt tggtatctgt ggccggcgcc ggagcacggc tgaaaatcta tctggacaaa
    28981 accggcggca ttatgggcgg tctgggcaac tggcggcaga ttcagccaat agaattgctc
    29041 gaggttaatg acagcagaaa aacatggtca tttttcgatg tttttggttc gaaaattgtt
    29101 ctcgaacccc cgctggtcga atatgatcag gccgttccca attttgagac cgctgttcag
    29161 ctttattatg aattctcgta tgacatacag caggccgagc tgattccctg ctggcttttt
    29221 aatgttgatt actacctcaa aggaaagctg atgctgacag ccgagacatt cattcctgct
    29281 tcgacgatgt atatggcgcc ggtagtggag attgtcaaac ccgaggattc ttccaagttc
    29341 cccgccggca gtatgatagg atttgactgc gaggttgctt ccggccttgg tacaccgcct
    29401 tacagcttcc agtggacttc cagtgtggac ggtgtgctgt cggaccagca gacttttcaa
    29461 acggatactt tgactgtccg ctgtccggat atctcaatgg actgccagcc gctgccgcat
    29521 accatcaccg ttactgtaac ggatgccaaa ggattgcagt cctcggattc gatacagatt
    29581 acgattattg gcccatgcga cgaatgcaaa gatccggctg actttaatca ggataagata
    29641 gtggatttgg aagattttgc ttactgggcc agccgcttcc tgatgcagtc aggaaagcag
    29701 gaatgacggt aaagaaaagg acaggcatca gttgcttgat gcctgtcctt gtgggtgctg
    29761 ataagagaca acaatctgac tccagtataa tataaaggcc ttagacattc agttctgaag
    29821 gcgcctgaat gtcttctgtc agccattgtt cggccagaat agaaaagtcc tccagcgcaa
    29881 caacgccgtc gcgattcaaa tcggccgaag gacggctgcg gatatacggg taggtttgat
    29941 gctccccaag ttcccaaata ttcacaaagt cccatccagc gctgatgtag gttgtgcttt
    30001 gcttcatctg ggctgtcgtc ttgccgatcc ctccggcgct ggtgctttct ccgctgacct
    30061 gcatatccca gaaagagtcg gctacaatgg agccgtcatt gaggcctacc agacccccga
    30121 cattcggaat gctgatactg ggacgctgta caattccggc tgcatagcac ttggcggcca
    30181 gagaattact gctgagcatc cagccgatca ggccgcctaa gacttcattg gggccgcccg
    30241 tcgtgtcgat gacaccgatt acagaggcct gcgaataaca gtaataaagc gttccgcccc
    30301 cgagatatcc aaccagtccg cctacatagc ggctgccggt aactgttccc atcgaccagc
    30361 atcccagaaa cgccgattgc tggagggtac agcatcccgc aattcccccg actaaagaaa
    30421 ctcccttaac ggttccattc cagctgcagg ccataatagt tgccgagtcc aaccgtccga
    30481 ccagaccgcc taccccaaaa cggccttcca cgaatgcatt ttctacatgc aaatcgcaga
    30541 tttctgcttc cccccatata tatccgaaca atcccacatt gtcggatccc ggctgactga
    30601 taaccgcatt gctgatagtc cagccgtccc ctcgaaacac accggtaaac tttgttgtac
    30661 tgttgccgat gggtgtcaat gtgactcccg ccaaatccag gggtccggtt agtttgaaat
    30721 gcttgtccca atcctgcggt gtggacatta aaatctgcca gtctgcggga gtagatatct
    30781 gataaggatt atctgccgtc ccgctgcccc cgccgctgta gattcctgcc gaagcaaaac
    30841 tcgccagaaa caaaataccc atcatcattg ttttcgtcat catcgcctct ttctccatag
    30901 aaaattgccg aaaaatcaga gcgccaacca gttgctgctc ttgaaagttt ctgtactaaa
    30961 tccgggtact cctcttgata caaccccagc caataaaact atcattatac tgtttaaatt
    31021 aggaaaaatc aagcataaaa aggatttgca aagggcttaa acagtgaaaa aaagggacag
    31081 ccgcctgaga gcagggctgt ccctatatgg agtgccgtcc aaacattatc tttattgtgc
    31141 cggctgtaca ctggtatcca gcagccattc ggaagccagc ttgacaaagt cgcctaaatc
    31201 cacgatgcag tcgccgtcta aatcatacgc aacaggagcc ggacatactg ccttgccggt
    31261 gatgtcggca tacgtttggg caatcgtcgc cgcatccaag ggataattat aaatctcaac
    31321 ttcgtctaaa acacagggag taacgctgga gccctcaaaa atcaggttgc ctgagctgcc
    31381 ccgaacacgt ccggccagtg ataccggcga cagagtggaa tttatgacac cggcagcttc
    31441 gtcatattcc tgaataatgc ggccgtcatc ccggctgtac acacggctca gaacaccgtc
    31501 aatatagact tttttaacag agccgtcaaa ggtgccgacg gcataatgcc agctgttgtc
    31561 gtaaacggta cggttgctgg cagtcccatc atcgttggca gttccccgcg ttgtaaagca
    31621 gattttatta agcgtgtcgc cccgatgccg aagctgccag ccctgtccgc tctccccgtt
    31681 gcgggcaacc attggtcccc aattggccgc aaacggcgcc tttatccagc aggccacagt
    31741 tatgctgtaa ttgcagatat caaagtagga tgactggtca gggtctgtat acaacatatc
    31801 ttcagcgccg gtgttgttat caaactccat gccgctgccc actatgccgg ctgtaaacac
    31861 cgggtctcca tacagcagcg aaacaggact gccgggcaca gtactgttgg gattgttttc
    31921 ttcaaatggc cagtaggcaa tgcggcgttt gatagccagc ggggctttgc cggacacagc
    31981 cggtatgccg gactcattgt ttacgataca ataataaaga ccttcatctg acattttagc
    32041 attcgcaatc gctagcgcag cttctgttac tcctgaaata tcgccgccat cggaaagctg
    32101 gatgtcgttt tggccgtcaa catacttaaa ccattgatat gtcggggttg aaatcgagac
    32161 aacagtaacc gtaaatgtcg ctgttccgcc ggtttcaata atttgaaaag caggatggcc
    32221 ggtaatgata ggcactgatt tttccgtctc aaaaatccag atatcgccgg tccgaacact
    32281 ggctgtgttg taaatagtat ccacacgcca gtaataggtc gtatcacggc tcagcgtcga
    32341 aggggtataa gaagcctgca gtgtttctgc atcccagaca agaatatccg ccgcaaaagc
    32401 gaaatttggt tcggctgctg ttttcatata caatttcata cttaccaaat tcggatctgc
    32461 ctgaccattg ggatcctgcg gcacagtcca aacaaacgat gctgtaatgg gcacatcagt
    32521 ttcacctgag gccggctggg gattatgcgg tcggtcggaa agcgagcccg gcacgaaggt
    32581 ttctgggaat gttgtggcaa tgataacatc gtcccatgtc gtcgcggctc cgctggcaat
    32641 ccgtacaccg ttgacccagt tccacatatt caggttatta aaaacagcat cagcgctggt
    32701 gtgattccac gcgatatcat aatcgccggt atccggatca acccacattc tggcggcatt
    32761 gtgattgtaa tccagcacac cgacaatccg atacggccgc ccgataacgg cctgaacgtt
    32821 cgtcaaaaca tattggccgc tgttctcatt gttgactccc agatatccca gacctccggt
    32881 ttgccaaggc atcccgaatt tgacgcgttc gccccattcc ccgtcctgag cgctgatgcc
    32941 cagccagccc tgaggagcat taatcgttaa agtcgccgca aagtatacaa tgccgacgtt
    33001 ttgaaatgcg ccggttcccc atgtatccga accaaattcc cgccgggcgg aaatccagcc
    33061 ggctccgctg tcttgcagcg acagagtccc gttaaccaca tttgtaatgc cggcctgatt
    33121 aagccacgaa gtcggggaag ggccttgcgg tttgccgact cctttccagt cccatccgta
    33181 cccgccgttt cttccggcaa ccggaccatc gggataatta aagaaatccg acccgacaat
    33241 atccccataa acgggaacca tcagaagcag catcattaaa aaaagttttg tctgtttcat
    33301 acgctcaccc tttcctcaaa aattacattt gttatttgct gcagctcggt gctaaccgaa
    33361 ccaccctatt tctgtttttc agtacccaaa tcccctcggc ggcttccgaa atctccctgc
    33421 ccaatctatg gcagtgacca tctggtaaaa gtactggaac cgataatgct gctgataaaa
    33481 attttttaag aagaaaggat atggatatga ctacattgtg cctgctgatt accgatgtga
    33541 tccgtgaaag ttgcccggcc atcattcttc tcatcatctg tcctctttat cctgaattta
    33601 tgattctaaa atcataatcc tcaaatagac agatagcagc actgccgctg cttcaacaat
    33661 attatatata gcaaccgatt gttatgtcaa tttccacttt tttattttga gaaggcaaat
    33721 cctgataagt tgtttaatct gctgtatcaa aaaaagttta taaaaaaatg cccccgggca
    33781 attgagggca tttggtggtt aagaggctga caaatccggt ttgaaagaac tctgctgcta
    33841 taatctgttt ttattactgt cttttgccgc taatcttcct gtttggtacg gctttgacgg
    33901 gccagcgcaa cggttccggt gcggacgata tttttgatgc cgtaaggccg gcacatttca
    33961 ataaaggcct caattttgct ttcgggtccg gctacctcta ccatcacata tttagggccg
    34021 atatcgacga ctttgccggt aaacatttca atcaaagcca agatttccgg ccgtttctcc
    34081 ggcgtgcagg ccacactcag cagcatcaga tcgcgggcaa caacatcctg accgacaaag
    34141 tcctgaactt taacgacctc aacaattttc aaaagctgct tgcggacttg ttcgacaact
    34201 ttgtcgtcgc cgataatcac aatagtcatc cgactcagcg ttgggtcgtc tgtgcggcct
    34261 accgccaagg aatcgatatt gaacgcacgc gccgcaaaca tccctgccac atgagccaag
    34321 acgcccggct tattcaaaac taaggcactc aatatatgct tcattgtatt ctccagcaaa
    34381 ggtttattgt cgcaattcta aacatatata aacagttccg aaagcgaacc aactgcggct
    34441 gttgtctgct gttgggaaag cttctgctta cgccatttca aagatgtcca gaccgtccat
    34501 ttcgtgcagc gactttccgg ccggcaccat cggccagacg ttctcctgcc ggtctacatg
    34561 gaaatcgacg acgcagggac gggtttcagc cagcatttgt tcaacagcac cgggaacctg
    34621 ttctttcttt tccacagtaa tacccacaca tccgaaggca cgcgccagat tggcgaagtc
    34681 cggattgacc agagagcttt gcgaataccg tccgccatag aaaagctcct gccactgacg
    34741 aaccattccc atatagccgt tgttaatcag cgcgacctta ataggcagat tgtacattac
    34801 agccgttgac agctctgtca gcgtcatatt gaagctgctg tcgccgtcaa tatcgataac
    34861 cagtttatca ggacaggcaa tcttgacgcc gatggcggcc ggaagtccat aacccatcgt
    34921 gccaagaccg ccggaggtaa tcagctggcg gggccggctg aatttgtaaa actgggcagt
    34981 ccacatctgg tgctgcccga cgccggtgca aattatcgca tcgcctttgg tttgacgata
    35041 gatttcttcg atgacatact gcggtttgat gacatcggtt ttacggtcat aggtaaacgg
    35101 gtgcttggct ttccaggcgg caatctgggc gaaccattcc tttcgctgtt tatattccag
    35161 ttcgccgatc atttcttcca gcacctgttt ggcgtccccg acgacaggaa tatccacgtg
    35221 caggtttttg ccgatattag ccggatcaac atcaatatga atgatcttgg catgcggagc
    35281 aaaagttgcc agattgccgg tcacgcgatc atcaaaccgc gccccaaccg agataatcag
    35341 gtccgcattc tgaaccgcat agttggcata ggccgagcca tgcattccga gcatatccag
    35401 cgattcggga cgggtctggt cataggcacc caacgccatc agtgtcatgg tgacgggaat
    35461 attggccttc tgtgcgactg cccggagaat ttccgaagcg cccgacagaa tcgctccgcc
    35521 gccaacgtaa agtaccggct tttgggactg gttaatagct gccgcggccg cactgatttg
    35581 gcggctgtgg cccttctggc ggttcttaaa gccgggcagc tcaatttccc ccttcggcgg
    35641 gataggacac tgagcaagct gcatatccac cggtatatca atcaacaccg gccccggacg
    35701 ccctgttgtg gcgatataaa aggcctcacg aattgtctgg gccagtgtgt tggggtcctt
    35761 gacgatcaca ttgtgcttgg ttatcggtcg ggtaatgccg gtggtatcag cctcttgaaa
    35821 ggcatcgttg ccaatcaatg aagtacgaac ctgacccgtc agcgccacaa taggaaccga
    35881 atccatcatt gcggttgcca agccggtagt taaattggtc gctccgggac cgctggtagc
    35941 cacaacaacc ccgggcttgc ctgttgctcg ggcataagca tctgccatat ggcagccgcc
    36001 ttgttcatga cgagggataa taaatcggat cggggcttcg taaagcttgt caaacagcgg
    36061 cagaacaacg ccccccaaat atccaaacat tgtatcgatg ccctgctcca gcagcatatc
    36121 gacaataact tgtgaaccaa ccttaataac tttaccatcg tcagatggag tgccgttttt
    36181 acgcatttcg atctcctcaa atatatctgt tgtacctatt ttgtcgtctt ttttatgagg
    36241 ttgagtggaa aaaatatgtc aacatatgac ttcagtcaga aagactatag cattcgcccg
    36301 ggaatatctg aaaatcacat tgaaaccctg tttaagcggt gtatcgccaa taacatcggg
    36361 tctggttttg taatacgcgg gggtgggcgc agagacaacc tgcggccaaa ggcaagttag
    36421 cataaatgaa ctccaatcct gtttaacatt tcgttattcc agtagccgca gggtcttagg
    36481 gcgatttccg acagggccga cctgactgct ccaagcaaat cagaccttct ttatcgggat
    36541 acgctgccct actttctgct gcactcaaac cacaatcttt acttgtgcca gggacgcaat
    36601 aagtactcac tgcgccagag caaatcactg accaaagcag ccagcttgtt aactactata
    36661 ttccagtttc cctatttagt caagtgtcga tgaaaaattt aatggatgaa ttttccagtt
    36721 gttaacgctt ttcaaaggga ttgacagtat tgcccgacat acaaataacg taaaactgtg
    36781 tcgatattgg cgaaatccag catcttcttg gacatttttt ctatagagcg gcactcattt
    36841 ctttatttcc tctgtgctgt atgaagagat tgcctaacca gttaacagca aatttgctct
    36901 gacaaagatg ataaaaaatt tatgaatttc ttaaaatcag gtctgaaaat agatgagttc
    36961 tcttattgaa ttatccaaat tgcagcacgg tctcacaaaa gttcaaatgg caagaagaaa
    37021 atgttttcga actgaaaaaa tttttttgta aagtttgtcc cggaaatagt cgatacaatt
    37081 ctttccggtt tggttattta tggcattaaa aaaatccaaa agcagttatt atcggacata
    37141 aacttcgtcc ttacgcataa aaatatagca cggaggtgct gattgaaaag cccagaccag
    37201 aagaggattc agcaggtatg ggaccatttt ctacaatatc atgacgatgt atcccgcaat
    37261 atccttttag aacattacct tgatttggtc aaatacaccg cccagaggct tagtacccga
    37321 tttcccaaaa gcatagattt ggcagacctt tatagtgcgg gaatacttgg cttaattgac
    37381 gcaatttcca aattcaatcc atccagaaat atcaaatttg agacttactg tgtacggcgg
    37441 attaaagggg caattctgga tgatattcgc aggaatgacc aattatcgcg tctggtgcgc
    37501 gctcgagccc agcagctcca aaaagtaaac cagcagctcc aaactcttta cggccgtatg
    37561 ccgactgatg aagaattagc cgctgcgctc ggattaaata aaaaagcgtt ttatgagctt
    37621 cttcgggatg ccaacgcagg ctgtctgatt tctctgaatg ctggtttttc ctctgcggaa
    37681 ggggatgaga cattcgggga attgagtatt tatgccaacc agaaaagtca ggatccattt
    37741 ctggaaatac aaaagcggga tttaagggaa tttctattca aaggtttttc ctctgaggaa
    37801 cggataattt tgaagctgta ttatttagag gaaatgacaa tgaaagaaat tggaaaaaca
    37861 ctgggtattt ctgaatcccg cgtctgccag attcattcat cggttatcgc ccgtctgagg
    37921 gcacgtttca accaatatat gcaggttttt acggtctaaa atccagcgta aacgccggtt
    37981 aaggccaagt ccgtttttta atgcctatcg ggtcttattt accgccgcgt ttattgacag
    38041 aatggatttt taggcgtacc atacacacat aaagaaacag aaaccgggat tctattatgg
    38101 gagtttctat ggattcaagt atatttaagg cctatgatat tcgggggctt tacggcgaac
    38161 agcttgatga gcaggccgct tggaaaatcg gtcatgcaac ggcccagttc ctgcgctcga
    38221 tggtgcacgg ctacaatcgc ggcttggcca acgcccagag catctgtgtc ggccgggata
    38281 tgcgaaccca cagcccctcg ctggccaagt ctcttattga agggatgaac gccgccggag
    38341 ccaatgtgat tgatattgga atgattgata ccccccagat gtattttgcc atcaatcacc
    38401 tcggcgtctg cggcggggtg caggtaacgg ccagccataa tcctgctgcc tataacggat
    38461 ttaagatttc cggccagatg gcccgtccta tcggtgctga taccggtctt aacgacatca
    38521 aacatattgc cacagctctg ctgcatactt taggccgccc taacggctcg gtcgaacact
    38581 gcgacctgac cgaagaatac aagcggcatg tcctgaagtt tctggattcc aagcatcggc
    38641 cgcttaaaat gattatcgat gccagcaacg gcatggccgg caaaatggtc ccgctgattt
    38701 tcggccagct gccgaatctg gatgtaacgg tattaaacaa agaatgcacg ggcacgttca
    38761 agcacgatcc caatccgctc atagaagcca atctgcggga actcaaagaa acggttcaaa
    38821 agcagaatgc agatttcggc gtttgctttg acggtgatgc cgaccggctg gtgatggtgg
    38881 atgaaaaagg acagcccgtt ccatgcgaca ttatgacggc cttgatggta ccgtattttc
    38941 tgaaaaaaaa tcccggctca actattgtct atgatcttcg cagcagccat gttgtgcagg
    39001 aagagatact caagtacggc ggtatccccc ggcgggaacg ggtcggccat gcgtttatga
    39061 aaaaaaccct gcgcgacagc catgcctgct tcggcggcga attgagcggt catttctact
    39121 accgcgataa tttctacgcc gattcgggaa tgattacgct ggtgcatctg atcaatatcc
    39181 tcggcgctac ctcgcagcca gccagcgaga tgattgcccc gctcaaacgt ttctattcca
    39241 gcggcgaagt caattttgag gtggaggaca aagccggcat gatgaaggag ctggcacgca
    39301 aattcggtca gggtcaggtt gatgacttgg atgggattac tgtgcagttt aaggattggt
    39361 ggtttaatgt gcggccgtcc aatacggaac cgcttctgcg gcttaatgta gaggccaaaa
    39421 ctcccgaact gctggaagaa aaattcgcac agctcaagtc tatactcggc cagccggtcg
    39481 cccattgact ttggagttcc taattgcatc ggcccaactg ctccaatgct gcataaggat
    39541 tttttgctcc aatagcaaaa agtcgtctgt ttctatctgc acaagtgttg cggccgaaaa
    39601 agataagatc tctgtgcaaa ggggggcaga gaaagtaccg cccccgcagg agaagacaat
    39661 gaaatcgatg cagtggtatg ttgtcgagaa aaggcagatg gtagcggttt ttctgtttct
    39721 gattgttgca gcagtttatg gagttgggca gccggcaaat ggtgaacaga tgcagcactt
    39781 ttcaaacaaa gtacaggtag gggcctatta ttacccttgg tatcatgccg cttccgatga
    39841 gcgcaaaggc cagtggagcc atgtaatgcg gctgcatctg aatccgcccc agcgcccgcg
    39901 atgcggcctg tatgactctg ccgatccgaa tgtgatacgg gaccatatcg cccagagtat
    39961 gcaggctggt ctggactttt gggctgtaag ttggtggggg ccgcagagtc tcacggataa
    40021 tgttttcaaa gatgctgttc tgaagcatcc cgaggctgga aaactgaaat atgccgttct
    40081 ttacgagagc accggccgat tgggcagttt ttccaagcca tcttaccgca attggataag
    40141 tgatctgaaa tatatcaaag agacttattt taacaatccc tgctatctga ccatcgacgg
    40201 ccgtcctgtg gtattctttt atctaagccg ggaatatttc agaaataaag gacaggaagc
    40261 actggcccag atgcgaaagg aaattcccga actgtatctg ataggggacg atgttttttt
    40321 tgccggcggg gcgtcggagg agtacaaacc cgaatgggcc gccaatttcg atgctgtaac
    40381 agtctatgat gtatacggtc aatcgattgg ccgatacggc aatacacaga aggccgtaga
    40441 aatcttagct gccaattacc ggcaggccaa gcaggctgct aacagtgttg gcgtcggatt
    40501 tgtgccgacg attgcgccgg gatataacga taccgctgtt cgcaaagggc atcccggcag
    40561 ggcacgctat tttgctgatg cggatgactg caaagaagga gaagttttcc gacagatgat
    40621 tcgcaaagca gcgctaccga atgctgatgt tcgatgcggc cgcatcataa tgattaccag
    40681 ttttaacgaa tggtttgaag acagtcagat tgaagctact gcaggcatac agcccgagac
    40741 tgccgcggat gattcaccgg atggcaaaac ctatacaggc ggaagtgtat atcgcgatta
    40801 cggtacgctg tatctggata ttttaagaga agaaataaag taatactttc tgctgccaat
    40861 actgtcagca tgccgatgga acgttactgt ccataaaata gaacctgctt ttcgagaacg
    40921 gcccggatct tgtttttgct cgatttcagc aattaataga atttagctcg tggttgcgta
    40981 aaaggcattc gcagatgata tacctgcatc gtgttttaac tgttgtttct tgctgacagc
    41041 aaagcgattt ttcggatcat ttgaatgata gcaatacgct tgattttgat gatttctaat
    41101 tatttcctgc cgggtagctg gatttatgag ccgataactg actataggaa ttgaatccct
    41161 gtcttcggga ataacaatgg caacattggg tcagaaaaca caagaaaact actcccctcc
    41221 cctgagacgg tcttttacga ttaagcagtc cgtgcctggc cgattaaaat gccgattcca
    41281 tttttatcgg ttcgttctcg accctcatga tgacttcttc gcaggacacg cacgattcgg
    41341 cacggagctt aatgttggaa tcttttccga ccagtacaac cttgaactgc ccgggcagta
    41401 tattgtaatc cctccgcaat tcatggctgt tttccggatg cagttcgtca gaacccacat
    41461 ggccgggttc gtgttcaaac acctcagcaa ttacaacatt gtgaccttcc agggtccgct
    41521 gacgctcagc caacgtttcc atctggattt gataaagatg atcttgccgg gatggggaga
    41581 acacaaaaag aaatgtcggt ccgtcttgta cccgatttga ttggaacgtt tgtgctgcca
    41641 tgctccgctc cttaatttgt acctttttaa tattaagtac ctatacgaag caagtcaata
    41701 aaaaaattga tattttcgga ctaaatttta aaagttataa gatttattat gtcgtatatc
    41761 tgttataggg aaatgattat atattgaatt cagaaacagg cctaaccata tcttgataca
    41821 taagtgcctg tttcagtttt atatgtgaaa tatcagatat cataatgcct gttaatgtta
    41881 aaaaattttt tatttaaata atagtatacc tataatatac tatataaaaa agacgatcgc
    41941 ttatagaaga aaaactatac atataataac ctgtatatag agataaatac ggctatataa
    42001 acggctatat aattagggag tgggaaaatc tatttccaag tgggctcaag cccaaattct
    42061 ttgaccaacc gcgaaagctt gcgatggtcg atcttaagca gtcttgcggc ggccatcttt
    42121 cggccacctg ctttctgcaa ggctttgatg accatccttt tgcggatttg gtcgaaagcg
    42181 gtaaattccg gatcttgcga gtccgcagtc agaaaatctc cagtcaagat gtcgataggc
    42241 aaatattccg gctcaataac tgggccccgg cttgcaatat gagcatattc tatggcattg
    42301 gctagttctc gaatatttcc cggccaatga taatccatca ggattttcaa tgcctgctca
    42361 gagagccgct tgaccggctc gtcataaagt tcggcctgtt tctgaagaaa atgattagcc
    42421 agaagcggaa tatcttcttt ccgctggcat aaaggtgaaa gctcgatttt gaatacgtta
    42481 aggcgaaaat acaagtcgac cctaaaggtt ccctgacgaa ccatttcttt aaggtcacgg
    42541 ttagtggcac agataatgcg aacatctaca gggtagctct gcgttgatcc aaccggcgtt
    42601 acccttgatt cctgcaaaac ccgcaacagt ttggcttgca aatctggttc caattcgctg
    42661 atttcgtcga gaaaaagggt tccgcggtca gccgctctga agaaaccgag ggtatcgctg
    42721 actgcaccgg taaaggcccc tttaacatga ccaaacaact ggctttcaaa aatggatccg
    42781 ctcaaagccg tgcagtcaac aggaacaaat acattatttg ccctccggct atgaagatga
    42841 atctgacgcg ccgtcatttc tttgcctgtg ccggttgggc catgtataat tacgcagcaa
    42901 ttccgcggtg ccacaatacg aacggtttcc ataaccgatt gaaacgcaac cgatcgtcca
    42961 actgcagaat accgccccag tgtattcgcg tagcctgtat cttttgaaat caatttcatc
    43021 tcccttctgc caaataccca aaacactctc cgtccggcag taatcactgg ctgaagtgtg
    43081 attagatgaa gtggattcct tcctcactct ctctccatct cccactgcct atcccacaat
    43141 tgtagataag acatctaatt ttatcaatcg tggtttctac ggtatttctt aaaaagccgt
    43201 ttattagcca aaaaataggg atttaatgta aaatttatta ttaagaattt atttttttta
    43261 agcttaaaga caaaaattgg cacttctgta ccaattctac taatgtacga acattcattt
    43321 tttgcataat atgcgacctg tggtcctcga ttgtccgtat tgaacgaccg ataattttgg
    43381 caatttcctt gttgcttttg ccttctaata tatataatag tatttgaagc tctgttttag
    43441 ttagtatttt tgagaccgta atatcgtaaa tagctccttc tatataatcc ttttcggcat
    43501 ataattgagc cgtgatttca acccatgcac ggctgccgtc gcagcggatt atttcggttt
    43561 cgaatcgttt gattgggcct tctttcaata gctcgagaag ctttattctc actttggggt
    43621 cggcataatg cgctgtggca ttacacttgt ccagacaatc ttgtttgctt ggatacccca
    43681 gaaggacagc caatgcagaa ttgcattcaa tgagttttcc atcttttatg tttgtgcgat
    43741 aaaggggtat ttgtgcttgt tcatagagtt ggcggtattt tttttcgttt tcaagcagct
    43801 ctttttcaag tgttttttgc tctgttatat cgcgatagat gactccaacg gcaatcatct
    43861 gtccgtttga gttccgaatt ggtgaaataa cagattggct gtaaacaagc cggtctccga
    43921 ccggcatagc gtcttcaacc tgcaatggct gacctgtggc aaaaacttgg tcgattctgc
    43981 tcatccaaag gcgcataaag tctggaatat gccctaatcc gtctcgaata tttttaccga
    44041 taactttatc ccgggtggtt tttacatgct caatggctgc ctgatttgca tagaggtagt
    44101 tataatcttt atcccaaatc agaatgcagt cggaggagga ttcaaacaag gccaatatct
    44161 gatctgtggc aggattgccg atgctttgcg taaaaaaaat attttcggga gaaccagata
    44221 tgtagttaaa aaaatgcgga ttatcccgca gaaacgaatc tcgtttatcc aaattcagac
    44281 aattagaaca cggatcttcc attacagttg ccctattccc ctcattttta tgcccttatt
    44341 aaaataaggg gctaatatat aagtacggct aatcatttta tcgagaaaat atgatttgtg
    44401 caatatttcc ccgccgatgt tggaaaacat cgttgttaag ctccatgtct gccgccaata
    44461 atattgatga attcataaag agatacgcag gaattgcctg aaaactgcaa atttttataa
    44521 aaaattgaca tttttttgca gcgatttatt gttcacaatc agccaatcaa atttcttgtt
    44581 tttcacaata ttgtttcata taattatttt acctattagg gaatagccag ttatgctcga
    44641 cgatgaaatc ccaacaaata agtccgaagg agttttttgt acgacacgat ggtcgatgat
    44701 ttcagaggtc agaaatccaa attcgaccag tgcacgctct atccttaatt ctcttattac
    44761 agactattgg aagccggttt actgccacat acgaagaaaa ggtataccgg cggacaaagc
    44821 caaggacctg actcaggatt ttttctatag tatcttgtat gagaaaaacc tgttcgcaaa
    44881 agcccaaaag tctcaaggac gctttcgctc ttttttactt acggccctaa atcattattt
    44941 agtaagtgaa tatcgttatg ctcaggcgaa aaagcgtata gaaaacaaag aaatattcaa
    45001 ttctgaaatg ctggatgata tagagatttc tggagttttt gactcgatga gggaggatca
    45061 atcgttcagt tatgcttgga ttgctgcttt gcttgagaat actactgccg ctgtcaaaca
    45121 gcatttttct ttactgggca aagagctgca ttggcttgtt ttcagcagac gggtattgga
    45181 gccgattgta cacagaacgg atccgccctc attggcacaa ttgtgtaaag agtatgcatt
    45241 ggaaaatgag actgtcgctt ccaatatgat tacaactgtc aagcgggcct tcagaaaggc
    45301 actccgtaca cgcattcggg agtatgtgta tacggattcc aatgtagatg acgaattgac
    45361 tgatttgatg gtttttttaa gaaaaaaatc gcaggacggc ggctgatgct gcgtagttgt
    45421 ttttgaggaa tcttcttgct gcaaactata tgataggact tataagacat accggccggt
    45481 ttgtatatta caggcgcgtc tatgaataaa gaaatgctgc atctgctgaa acctgaccaa
    45541 ttgatagaga tgctgtcaat cgggatggat ggtgatccgc ccaactcggc agaactggtc
    45601 agtgatgaac agatacgaga ctatttttgc catctgataa accgcaaaat cccctgtcaa
    45661 tccagtattg tggattcgat tttaattttt ttgcgtgaaa atgagcgata cggacatctt
    45721 gtcaaaggcc gtacacttag cgaattgctg ctggattctt cctgtccggc gggtttgttt
    45781 gaagctgtca aggaaatcag caagagagtc tgtcagacag cgatttccag aggtgagaat
    45841 gctgttgcgt cggccattta ttatgccgct atcgccgctg ctttgattta ctgtaacacg
    45901 aagatatcaa gtcattcatg ggagacgctg aaaacatcct tcgaagattt gctggccaag
    45961 tcttggacgg ggccgttttc agaattgctg gaaaaggccc gccagcggtg tgaaaaagaa
    46021 gccgcaatga aatgaatcct aacctgcctt ttgatccaca gccaagtcca gatgatgccc
    46081 tgccgatcag ccagacagct ggcggacaag aaccgggctt ttctttaccc gcaccgaaaa
    46141 tcgacggcta tgaaatactg cacaaaatag acgaggccgg gcaggggcag atttgggcgg
    46201 cagtccagca gagcacaagc cggaaagtgg ccgtcaaagt tccccgcttt cagggatttg
    46261 gagcacggaa ggcctttttg cggtttgaac gcgaagtgca agttattgcg cgtctcaaac
    46321 accccaacat tgctcgtgtg tacgacagcg gtgtaaaccg cggcttttat tactatacaa
    46381 tggagtatat tgaaggcagg catctggacc gctatgctgc cgaggaaaaa ctatcccgcc
    46441 gcgatattct tatgctgttt gagcgggtgt gtcgggctgt tgaatatgcc catcagaatg
    46501 ctgttgttca ccgagatctc aagccgtcca atattctggt caccgccgac ggacagcccc
    46561 atattgtcga tttcgggctg gccaaggaaa tggatgatct gaccgttacg atttcgatgg
    46621 acgatctgcc ggttggcact cccgcgtata tgtctcccga gcaggccgaa ggccgcccag
    46681 ttgatacgcg ctccgatatt tacagtctgg gtgtcatact gtatcgtctg ctgaccggca
    46741 ctttcccgca cgatatgtca ggcagccggc aggaggttct ccggaggatt gctcaagatg
    46801 atattatcaa gccggttttt ctgacccaaa agccggacaa ggagctggaa tatatccttc
    46861 gcaaagcttt ggccaaagaa gcccagcgcc gctattccag cgtccaagag ctggcacagg
    46921 atattgaaaa ctaccttgcc ggccgtccgc tgctggccgg ccccgaatcg gcgttttata
    46981 ttgtccgaaa atttatttgg cggcatcgga cgctggtgct gggtttggca ggcggactta
    47041 gtgcgctggt cgttgctttg gtggcaatat ccttgctgtt ctggcaggcg cagaaggcca
    47101 agcgggaaat ccagcaagct cttgaacggg aagaacggct caactacagg aataccatcc
    47161 aaatggcgca cagcttgctt gaaaacagcc gttatgctgg agtccgccgg atgcttgaag
    47221 atacctatcc ttcaaagcga agctgggaat ggggctacct gatgaaatat tgccagctgc
    47281 cggaaaaatc tctttcgctt gacattgccg tcgatacagc agcgctttca acggatgggc
    47341 gttttctgat tctgtctgat tccctgacgc agacggctat gctgatggac agccgcgaag
    47401 gcacggtggt ctggcagcag aaaatccccg gttatattac cggatttgtt caggccgctt
    47461 ttgatccctc gaatcggttt gctgccgtgg cctcgggaac ggagcttgtt gtttttgaca
    47521 tagccgacgg acatattctg tttaaatcga aaggaatcgg gtttctatcc tgctgtgccg
    47581 gtccggcagg attatatgca ggcacatttg acgggcatat ttttctgtat gatacccaga
    47641 attggcaggt tctgcagacc gcagtgctga aggaaaaaat ctcactgctg gctattgccc
    47701 cggacggaac acagttggcc gctctgtccg ggatgcggac gattaccctg cacgatccgc
    47761 atacgcttca gcagcaggcc gccttgactg ccgaggattc tgtttatcag gatttgctgg
    47821 ttttgtccgc acacacagtg gcctcggcat cgggcaatgg ttttacgatt gatcatctga
    47881 caacggctcc gcaagaacgg tccgccggtc cgatccgaga ccaccttcag gaaataacag
    47941 cactggcact gtcagccagt caggataaga taataaccgg cggcttagac ggggttgcac
    48001 atatttatga tgcccgcacg ggacagatgc tcgcaacgat ttaccacgac agtcccattc
    48061 aaaaggtctt cttcctccct tcggataagg ttattaccct cggtgccgac ggcagaatta
    48121 aatggtggag tggttgtaag catgaaccgc agcatcctga cgtgacgatt tccctgccct
    48181 tgtctgccgg tgtttggcag ctcgactatg acagcgacgg cagacatttg gtatttgcga
    48241 cttcggactg tccccagatt gtactggcag acgcccagac ggggagcaca cgggctgtcg
    48301 aggcagaggg ttacagcgag gaaataaagg aattttcgcc gcgggttttc ttccggccgg
    48361 gcaaatcaga attgatactt aaaatcaaag attcgctgcg catttatgct ctcaaagaag
    48421 atcgttttga ggaaatccgc agattccctc tgtcggcacc ggttggcagc gcagcagtag
    48481 atccgaaggg acgtttcgct gcagtggctt acctgcgggg tttcggcatc ctgatagacc
    48541 ttgatgccgg cagcatccga cagctggcct atggaaactt ggcggcatcc tatgcggtct
    48601 tttcgccgga tggctcccgg attgtgtttt tggaagccac cggttccaaa cgggctgcga
    48661 taattgattc gcagaccggc cggctggttt gtaccctttt tgccgatttt cgccggcaag
    48721 cacaagtagc agcattccat ccgaatttgc ctattcttgc catcgccagc agcgacggcg
    48781 taattcaatt atggaacata gataccagcc gcaagatagc agaaatgaca gaaaacggat
    48841 taggagctgt ttatggactc cagttcagcc gagatggcca gcgtctcctt tccggttccc
    48901 atgataaaac tattcgtctt tgggactgga caatcggttc agttttgctg aaaatcagtc
    48961 atccggcgac gcctgtggat gtctgtttca gcccggatgg cttatcgatt gcttgtgctt
    49021 attatatgcc caattcctgc cggatttaca gggcgattcc tctggctgtg ctgcagggca
    49081 gtcaatcgga acaataaaaa accccggcct tgaggccggg gctttctgct tgtgcgtatc
    49141 ctatctttgc tgccgaagac ttcggttatt tgacaaattc tacatggaga tagtgcgtcg
    49201 agcaggaaat gcacggatca taagcccgca cgagcatttc cagcttctgc cgaagagcat
    49261 cttggccttc atgcatgtat tgggggacca gcttctcaaa atcgttctgg atgttggcat
    49321 gattctgatt ggtcgggatg cagcagtttg cccacgtgca ttcgcctttt ttattaaact
    49381 cataagcatg aaacagaatg ccgcggggcg cttcaaccgc tgcatagccc cggccggcgc
    49441 gcggagaaac cctcagtttt tctggtttga gccctttggt cagaaggatg tcgatcagtt
    49501 ccagactggc ctgtactaca tgagccgcct cggccagctg ggcgacgctg ttcatatacg
    49561 gattgcagca gccctttttc aggccgaaca tgtccgccac agttttgccc agcgggctta
    49621 gatattcggc ctgattgttg aaccgcgcca gcgctccgac aaagtacgag tcccgatgcc
    49681 acttggccca cttggccgtc gattgcggca ccacatactc attggcaaca ttttcccact
    49741 gattgaccgg catatcggct ttgccgttga gatctgtgct ggtgatgtct ccatgataga
    49801 aagagtaggc agacagattg tttttgtaaa acggattggt ctgccgcagc gacacatatt
    49861 cggtcggacg ctcaaaggcc ggcagattgc cggcaacgct taggacaacc ttgcagatat
    49921 ccaccagatc cggcaccaca tcttccagcg tcttctttag gtcccgcagt tcctgctcgg
    49981 tcggaatgcg cgtcattccg ccgggcgtca gcgtaaccgg gtgggtaatc cgcccggcca
    50041 gcagtgctga catctgattg gctacccggt gtgccttgat gatttttttg acggcttcgg
    50101 tggctttggg caccagcggc acaaccgatt tttcaccgaa caaatccggt gccaccaagt
    50161 agccgacgtg cagcacatgg ctttgaagct gctcggaata atgcatcaga tgacgcacaa
    50221 gatctgtctg ttctgtaact ttcagcccca gcgcatcttc cacggccttg gtggccacca
    50281 gcgaatgcga aatcgagcag atgccgcaga tgcgcgacac gatcgtctgg atatcgtcat
    50341 agcggcgtcc ccgcaccatc gcctcaaaaa aacgcggagc ttcgggcacc tgccattggc
    50401 atttttcgat ggtcccgttg ctgatgttga ccacgatgtt gccgtgccct tcaacccgtg
    50461 tgatgtgatg aacgttgata ttaatcgttt tgctcatata cggaaccctt gccttacttg
    50521 gtgttgaaca ataccatttt ctgtttcaag tcatcgacgg tcaaatcgta tttgtccatg
    50581 acttcctgcg ccgcctgcag attgggattg tccaccagtc cgcggcagcc gaagcagcga
    50641 aagccagccg ccggacaggg agcattgcag ccggcccgga taatcggtcc aaggcagatt
    50701 tcgttgtatt cataccggca gatggtttcc cgtgccttgc actccacaca
//
LOCUS       DAOTBO010000011        50536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_563_length_50536_cov_5.779184, whole genome shotgun sequence.
ACCESSION   DAOTBO010000011 DAOTBO010000000
VERSION     DAOTBO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 50536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50536
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_563_length_50536_cov_5.779184"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLE88_02430"
     CDS             complement(<1..878)
                     /locus_tag="PLE88_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOM75354.1"
                     /translation="MQIKINLDNHLAGPGGIREMIAIALPMVISSACETTMTFTDRLF
                     LSRLGPEYMSAAMAGGLTSFVMMTFVLGLTGYTTALAAQYWGANQKKRCPVAVTQALI
                     LSLLAYPVIVAARPLAFWLFDVIKVPSEQLMYQQQYFNILLNGVLIGLLRNCLSSFFS
                     GIGKTRVVMVSALTSMAVNVGANYLLIFGKWGIPPLGIRGAAYGTLIGGFCGLMVLAA
                     VYLGSSVRREYSVAASFRWDSDTMKKLLRFGYPAGLEFFLNLLAFNLLVMILHSHSAV
                     TAAAIPLVFTCVLFSF"
     gene            complement(869..2239)
                     /locus_tag="PLE88_02435"
     CDS             complement(869..2239)
                     /locus_tag="PLE88_02435"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOM75355.1"
                     /translation="MMQAQRIRNRNNKPIQQRGFVLYWMQASQRAEWNHALEYAILQA
                     NKRIKPVLAAFSLTDDFPEANQRHYAFMLQGLRETQAALAERHIQLVILRGNPVKTIP
                     QLARQADMLVMDDGYLRCQRQWRDSIALSADCLACEVAANLIVPAEEASDKENFSART
                     FRPRIHKQLPQYLIPLQHSKPRCSSLNLQFSSYDISNTEQTLKQLNIDHSIAPSIFYT
                     GGTSQAKQRLRKFIQEKLDAYPDHRSDPLQNCQSHLSPYLHFGQISPLYIALEILKTD
                     SPGKDSFLEELIVRRELSHNFVYYNRRYDQFDALPHWALRTLKLHAKDKRLSIYSLDE
                     FEHARTHDPFWNAAQTEMALTGKMHNYMRMYWGKKIIEWSRTPEEAYHIALHLNNKYQ
                     LDGRDPNSFAGIAWCFGKHDRPWGRHPVFGTVRYMNAAGLKRKFNPAAYADTISNLRL
                     AEKQCR"
     gene            complement(2258..4123)
                     /gene="typA"
                     /locus_tag="PLE88_02440"
     CDS             complement(2258..4123)
                     /gene="typA"
                     /locus_tag="PLE88_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOM75356.1"
                     /translation="MTFTSHIRNVAIIAHVDHGKTTLVDQLLYQSGMFRSAELEKLAG
                     VQHGLIMDSNPLERERGITILSKNCAIHYTDPDGEEYKINIIDTPGHADFGGEVERVL
                     KMADGVLLLVDAFEGPMPQTRFVLSKALLHKLKPIVVVNKVDRPDSRPDDVVNEVFDL
                     FVALKADDRALDFPILYASGRNGWATRDLAQPGTTLQPVFDAIIEHVPAPKDDPDAPL
                     QMLVTTLDYSDYVGRIAIGRVFAGTVSQSQKITVIDNQGRHTQQKVLQLYTFDGLGKK
                     TVDTVIAGDICAIAGLDPVDIGNTVACADEPSPLPVIAVDEPTMNMTFRINDGPFAGR
                     EGKYVTGRHIRERLEKELQVNVALRVEPGQRADEFLVSGRGLMHLGILLENMRREGYE
                     LCVGKPSVIIREIGGLRQEPIERLVIDCPADCQSSVISLLGDRRSELISMDTKAGADN
                     YIHMEFMIPSRGLFGLHARVMTATQGRAVMHHTFERYEPLRGSIPQRQAGVMVATETG
                     IVTAYALDALYDRGIFFVEPGEMVYEGQVVGEHCKDNDIPVNPARAKQLTNMRATGKD
                     DAARIRPARKMSLEATLEYIQDDELVEITPKSIRIRKRLLKEADRRRESRKSKNQ"
     gene            complement(4294..4527)
                     /locus_tag="PLE88_02445"
     CDS             complement(4294..4527)
                     /locus_tag="PLE88_02445"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOM75357.1"
                     /translation="MRYFEMTEKIGQAAGIIWHLLAKAANPINITDIPKRTNLTSQMA
                     YQGLGWLAREGKLEYTQKGRSIYVSLLSVPSSN"
     gene            4730..6418
                     /gene="glgP"
                     /locus_tag="PLE88_02450"
     CDS             4730..6418
                     /gene="glgP"
                     /locus_tag="PLE88_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOM75358.1"
                     /translation="MKETKKIAYFSMEIGLDPAMPTYSGGLGVLAGDTILSAADMKIP
                     MVAVTLLHRKGYFYQRLGADGWQTEEPVEWIPEDFTRELEPRIVVNIENRPVKVRAWE
                     YIVKGFGGYAVPVYLLDTDLEENAPQDRVLTHYLYGKDARYRLSQEIILGIGGIRMLR
                     ALGYTELDRFHMNEGHASLLTIELLDERIQARKEDQITEEDIAEIRRQCVFTTHTPVA
                     AGHDKFPMDLVREVFGSHKAFMLENVFCCENQLNMTYLALNLSHYVNGVAKQHGEVSR
                     QMFSGYKIDAITNGVNATRWVCPSFQKLFDKHIPGWREDNFSLRYALSISCSEITQAH
                     QQAKRELIQYVNQQSNLGMDTEVLTLGFARRATSYKRADLIFTDLQRLRSISVNEGRL
                     QLVFSGKAHPNDTEGKELIKRIYSAARQLRPEIKIAYIPNYDMDLGLLLTSGVDVWLN
                     TPQPPMEASGTSGMKAALNGVPSLSILDGWWIEGCIEGITGWAIGENHHLEQHSDRAA
                     DAEHLYQKLEQVVRIYYKEQARFCSVRTHAIALNGSFFNTQRMMQQYVLKAYFG"
     gene            complement(6422..6805)
                     /locus_tag="PLE88_02455"
     CDS             complement(6422..6805)
                     /locus_tag="PLE88_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75359.1"
                     /translation="MEWIGAFLVSGLVCWGTIKQTVPFTSLINLGQKVRSSWSSQQPQ
                     AIQQGQYGQRRGFGGGTNAADSGGFGQQTLIQTCQELGIDINTALETLKKKGIQASPN
                     YTIRRIADENRIRPGDIRQHLESAR"
     gene            complement(7167..7760)
                     /locus_tag="PLE88_02460"
     CDS             complement(7167..7760)
                     /locus_tag="PLE88_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75360.1"
                     /translation="MAKKKSKKLTAKQLEFYRQLLIEKMQEISGDVYTMEESIFQNGG
                     ELSSMPVHLADIGTDSYEQDFNLDLMAEEKKILMEIQEALKRIKDGTFGICEGLGIPI
                     EEERLKAIPWTRYSLEYAKMLESGQVIKRHNFKRRPIDIDRDEDFESEEEVETDADEL
                     VGEDIDLEDSMESLETIEEDEDADYENEDIERQRDSA"
     gene            8285..8839
                     /locus_tag="PLE88_02465"
     CDS             8285..8839
                     /locus_tag="PLE88_02465"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOM75361.1"
                     /translation="MPNSMVPTRMFLTKGVGRHKYQLKSFEDALRQAGVAQQNLVQVS
                     SILPPHCKIIRREQGIELLKPGAISFCVMARCDTNEHGRLIASSVGVAIPKNRKKWGY
                     LSEVHGHGMKKKEAEDMAEDLAAGMLGTTLGIELDPDLAWSEKEQAYKSSGLFVKTTN
                     ITQTATGRKGLWTTTVALAVFIFD"
     gene            8842..9708
                     /gene="speB"
                     /locus_tag="PLE88_02470"
     CDS             8842..9708
                     /gene="speB"
                     /locus_tag="PLE88_02470"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOM75362.1"
                     /translation="MGRARRTQFGDFSPRFYNPETAAIAILPVPYDGTSTWIKGADKG
                     PQAILDASYNLEFYDIETDSEVFRKGIFTEPPVEGFADPVRLAGVVRQRMSRILEQNK
                     FPVVLGGEHSVSIGVIEAMADKYKDLTVLQLDAHSDLRDSYEGSTHNHACVMARAKEF
                     AAVVQVGIRSMDISEKKNMDWGRVFFSHDICSRTDQQWIDQAVEHLTENVYLTIDLDV
                     FDPSIMPATGTPEPGGINWYEAVHLIRAVCSRRNLTGFDVVELCPREHLWASDFLAAK
                     LVYKTLTYKFCL"
     gene            9926..10213
                     /locus_tag="PLE88_02475"
     CDS             9926..10213
                     /locus_tag="PLE88_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75363.1"
                     /translation="MFETLDKLLLAGLGAMSMTKEKAEQIFDEYVKKGQAQKEQRAGF
                     VKEVMEHAEKAKRDLEKIISEQLEKAMSKQPLATKEDIKRLESKLDQLLSK"
     gene            10225..11919
                     /locus_tag="PLE88_02480"
     CDS             10225..11919
                     /locus_tag="PLE88_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOM75364.1"
                     /translation="MRNPLFSVRRTFAHMVRYRHIMTVLIKYGFEEIAGIFARRLKIG
                     LGSKGLPTTRMQNLAQTSLAARVRLACQELGPTFIKLGQLLSTRPDLLSDEYIAELER
                     LQDQVAAEKYEDIRLMVKQQLGGFPEEIFERFDAEPIAAASIAQVHRARTKEGDEVVV
                     KIRRPGIVKTLRTEMEILADIAGILKTRLPTPETFNPVRMVQEFSEAVTKEVDLANER
                     RNQQRFIRNFADDPSVHVPRVFEKYSSEGVLTMEYIEGIKPNRIDQILAAGLNPKIIA
                     ARGADFVLRQVFEFGFFHTDPHPGNFLILDGNVLAPLDFGQVGRLTKQDRMLLQYLVL
                     AVVEGDVSRIVHGLERAEMLDEETDLAELSRDFEEILETYSNLPLKDIPFGEAIRRMF
                     DVIRHHRIEPPRDFTLMLKCLMTIESFANDLDSNFQIMEHLKPYARRFTLQQISPEAL
                     FRQARTAARSASELASRLPEDAAAIISKFRRGKFKIHVYHDHLEEMEQTLDNSSNRIS
                     FAVIIAALLIASSMLVPQSGGVLGIVTFQTLGILGYLTAMVMGLWLLFSIIRHRRF"
     gene            12024..13127
                     /locus_tag="PLE88_02485"
     CDS             12024..13127
                     /locus_tag="PLE88_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOM75365.1"
                     /translation="MMDMHKEYQRRISKVRQQLRDAKADSFIATAVENVRYLTGFRGH
                     DSWAVILPKAVILITDSRYTEQARGECVGCRIIERKDGLVKTAAALLEKQSHIETVGI
                     EDTCPLSLFKTLRKELPVQVMPTKNVIENVRLLKTDGEIQLLRKASRIAFDAMDWALG
                     LLMPGMTERQLTALYEYRVSEYKAKVGFETIICFGPNGSRNHHQPGERKLRKNDTILI
                     DFGVNYEGYISDMTRSYAFGKVSNFYRKVYQTVAKAQAAAIETICAGVKMADVDAAAR
                     KVIEDSGLPVYGHGTGHGIGLQVHENPYLTKTDKKGRLQAGQVITVEPGIYLPGQLGV
                     RLEDDVLVTETGGEVISRDERFDIHIDKVPQLG"
     gene            complement(13497..14087)
                     /locus_tag="PLE88_02490"
     CDS             complement(13497..14087)
                     /locus_tag="PLE88_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HOM75366.1"
                     /translation="MRRHIIIIIVLTSFAVLTGCGPSSSKYTLSERRRIIDDMAASSL
                     QHLYTIKPSARDEVAQAAGYAVFSNANVTVLFASGGAGYGVVVDKTTGVKTYMRMGMG
                     GLGLGLGAKDYRQILVFKSRDAMQKFVGTGWDVGGQADAAAKIGETGGEAGGEGTLRN
                     QISVYTITENGLMLQATITGNKYWIDDELNQPSLVR"
     gene            complement(14120..14680)
                     /locus_tag="PLE88_02495"
     CDS             complement(14120..14680)
                     /locus_tag="PLE88_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HOM75367.1"
                     /translation="MKRSSLLWMSFLTLLVGCGPSNQLTNEEKRSAIDAMAAETLERL
                     YHEKASVREEIEKAAGYGAFSNANVNIIFASAGGGYGVVVDKTAGTKTYMRMGMGGLG
                     LGLGAKDFRQVLIFDTQETIKKFIESGWEFGGHADAAAKTPDKGAEASAEGMIGAVKV
                     YSMTEAGLALQATVTGTKYWKDKELN"
     gene            15133..16170
                     /gene="amrS"
                     /locus_tag="PLE88_02500"
     CDS             15133..16170
                     /gene="amrS"
                     /locus_tag="PLE88_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOM75368.1"
                     /translation="MDNGQANLKDAVLWEKKDGKVQCLLCSWKCLISKGAVGHCKVRK
                     NINGHLYTLNYDKLCAANADPIEKKPLFHFQPGSRSFSIAAPGCNFQCVFCQNWQISQ
                     MPYQNILEGTFFSPRKIVEAALDDRCRSIAYTYTEPTVFMELCCQTARLAKAKGLANV
                     FVSNGFMSQEAVDFVKPWLDGINIDLKAFSDDFYRDMCKARLQPVLDTIRYIAHHTDI
                     WMEITTLIIPGKNDSDEELRSIAQFIVQEASADVPWHISRFWPQYKLTNVPPTDSTVL
                     DRAYDIGRQAGLRYVYVGNMPGARAESTFCYSCGTLLIERQGYRIIQNRITDSRCPVC
                     GADIAGFEISP"
     gene            16227..16856
                     /locus_tag="PLE88_02505"
     CDS             16227..16856
                     /locus_tag="PLE88_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOM75369.1"
                     /translation="MANLLYIQASPRQERSKSNQVASAFLRHYAKVHPQDKIGTVNVF
                     QEKLPTFDGLTVQAKYAILHGQPHTDAEQTAWSAVETVIRQFKQADKYVFSLPMWNFG
                     IPYRLKHYIDIVVQPGYTFSAGPNGYEGLIRDKPAAVIYARGGAYRPGTDVQAFDLQK
                     KYMDLILGFMGFEKIYSIVIEPTLHGSPDEVQKIVESAVEQACRLAEVF"
     gene            complement(16853..17518)
                     /gene="plsY"
                     /locus_tag="PLE88_02510"
     CDS             complement(16853..17518)
                     /gene="plsY"
                     /locus_tag="PLE88_02510"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOM75370.1"
                     /translation="MSVMMELITLIMAAYFLGAVPFGLLIAKAHGKDLRCIGSGNIGA
                     TNVVRALGRSWGILCFILDMLKGLIPMLAVPVLGLVGPQPKAYELTLWLLVGCAAVLG
                     HIFPVYLKFKGGKGVATSLGIVLGLWPYYTFCGIAAFAVWIVCVLLWRYVSLASILAA
                     VSFPIFLTIATSLFKDWGLATLWPLLAGAVLMSLLVVFRHRANIRRLLSGTESKIFQS
                     KQI"
     gene            complement(17509..18669)
                     /locus_tag="PLE88_02515"
     CDS             complement(17509..18669)
                     /locus_tag="PLE88_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM75371.1"
                     /translation="MIKAAILIERAEVKLGGAERSVSELVGELRRQGIEAVILAAKGT
                     PCEYLHILCQDYPGRRVPLRIFEAALQAHLENHFYDIIHSTLPTAVADIYQPRGGSYR
                     EAMLRNTASYANPMQRLLKRSTHLLNYRRTQYLKAEERLCRQHNQTIIAALSHYVKSQ
                     FQKHYTLPDSRITVIPNGISLPQTLDQSEAQKIRDSILQSVPVPAGKTPILFLFAAHN
                     PRLKGIGPLLAAMRMLRENPPSDVHPVLAAAGFSPTAYSAPNNTVFLGTHSCMNAIIS
                     ACDAAVLPTFYDPCSRFVLEALALAKPVITTRFNGASERYTHLRHGFILDRPDDVHTL
                     TYALQYFCSMEKINLARKAILDDNLRVAVSIEQHVNLLKALYRNILAEKKCQ"
     gene            complement(18674..19531)
                     /locus_tag="PLE88_02520"
     CDS             complement(18674..19531)
                     /locus_tag="PLE88_02520"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOM75372.1"
                     /translation="MTSPFIQPEPGFYVLPSYSRILAETGLDSLETVICCKKGQMLSK
                     SSLAAWRHRIRLQLANGQTAYLKRYISPPFFVQLKAWIQHRHRALLAEYDKGPSEELT
                     RAAVLTPQVIAYGGRWNGWSEKCSFIMTLEIPHGRSLEQKLPDCFETSPAPMQRQAKN
                     ECICRLADFVRRFHATGYRHQDLYLSHLFLSDEQILYLIDLHRTFKPRMRSRYYQVKD
                     IAQLHYSSPAAKISRSDRLRFFRAYVQREKLTAQDKELLRQVHSKALCMARHNRKHGR
                     ICPFQEKDA"
     gene            complement(19528..20550)
                     /gene="waaF"
                     /locus_tag="PLE88_02525"
     CDS             complement(19528..20550)
                     /gene="waaF"
                     /locus_tag="PLE88_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOM75373.1"
                     /translation="MHPSKVLIWLPSPLGDAIMATPALRAFRELFKDARICFLASSFT
                     REILSPSPFCDEWLTIDRHFVRQIKVLRQQQFDAAILLKNSFGSALCTAMAGIKHRIG
                     YVREGRWLLLTDRLLPPRENGVFKPIPMIEYYHSIATMLGSRQAVSPPELSVEDSAIA
                     EVYQKIPALKNIDGPFVIFVPGGAFGPSKQWPAASYAKLADKLYTAFHATVLLSVSPA
                     KEETEMAEAISRQAACHMLNLAKTPLSGGQLKALYSLADLVIANDTGPRHIAIALNKN
                     VISLFGPNNPQWTQTGHNKEIQIVGRAPCVPCGRPRCLMKKHLCMESITVDEVFEAAC
                     RFLKHP"
     gene            complement(20570..21640)
                     /locus_tag="PLE88_02530"
     CDS             complement(20570..21640)
                     /locus_tag="PLE88_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM75374.1"
                     /translation="MGHKAVFFDRDDTLIEDPGYIRSPDQVKLLPGVARSLIQLKKMG
                     YLLIVVTNQSAVARGMITEQELEEIHRRLKNLLAAEGAYLDGIYYCPYHPEGTVAQYA
                     KESNLRKPEPGMLLQAAREKDIDLSRSWVVGDRYLDIRMGRAAGCYTILVDVPGKQRE
                     KLPDDPEPDRKAVNIREAVNIIRMHEFHQKARSGRNGESSAFTETAQRAAVPEPKESQ
                     ETDAQPPQQASDPGLEGAKSLSSDEVITLHETSGFESREFSTKAAENDQPEDAGQTHR
                     LFEEILRHLKRMDRAGFYPEFSVFKLLAGMTQTVAVFCLIVSIGFWLNPKVGYEPVLI
                     MIGYAIMLELLVIALLMMHGRD"
     gene            complement(21630..23129)
                     /gene="rfaE2"
                     /locus_tag="PLE88_02535"
     CDS             complement(21630..23129)
                     /gene="rfaE2"
                     /locus_tag="PLE88_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOM75375.1"
                     /translation="MIYTNLLKAVTNLGCPRVLLVGDFMLDSYVYGDALRISPEAPVQ
                     VLKVVRREYAGGGASSVAADITTLGAKCFCIGAIGKDRSGERLLEILKQLGADTEGLI
                     ALDSRPTITKERIIGLAQHRHRQQLLRIDEECIGPLPANIYQQLLSVYESRLPHCNIV
                     CLQDYNKGMLEKDFCQQLIALARQAGKRVLVDPPLDTDYSKFRGASMITPNRKETCAA
                     VGFEIRSPDDAARASLMLREQLDLEMAVITMDKDGAYLRTQTLAQHFPTLPRMVYDVT
                     GAGDMMLAMLAVALAAGCDAVTALQLANIAAGLEVEKFGVATVSVDEIVNEIISRHRG
                     KMGKIHTVEDLIKQLAYHRQQKETIVFTNGCFDVLHRGHIEYLKFCKQQGHIVVVGLN
                     SDASVRQLKGAGRPVNNQHDRAAVLAALESVDYVTIFDQLNPLELIKQVRPDVLVKGA
                     DWAQKGVVGREFVEACGGRVVLAPLVDGKSSTATIEKLKELERNQDYGA"
     gene            complement(23126..24142)
                     /gene="lpxK"
                     /locus_tag="PLE88_02540"
     CDS             complement(23126..24142)
                     /gene="lpxK"
                     /locus_tag="PLE88_02540"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOM75376.1"
                     /translation="MRIILRPAGILYGLGMRIRNLMYDLGILSSCSVEVPVISIGNIT
                     TGGTGKTPLVIWMCNYLHQKGIRCAILTRGYKTQPEQISDEPALLTKACPGTAVVVNA
                     DRISGARKAIEQHQAQVLVLDDGFQHRKLKRDLDIVAIDATCPFGYNRVLPAGLLREP
                     KSCLKRASAAVITRFDQADEAQIGRIEQEIHRYAPGIPIAKAAHRHTHAATYSNAAIE
                     LDQLRGKPVLAFCGIGNPNAFFDRLRRNGIDTVETIAFNDHHLYTTQDLKQIFSRADA
                     CGAEAIVCTQKDWVKSALLAPDRKDRIFAYLVMELEFWDGLDTITGLVDNLPIGNLNQ
                     TAKP"
     gene            complement(24279..25358)
                     /locus_tag="PLE88_02545"
     CDS             complement(24279..25358)
                     /locus_tag="PLE88_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOM75377.1"
                     /translation="MTKVTLIMGDGIGPEIADAARRCIDATGAGIEWDIQQAGVDIME
                     TAGTPLPEATLESIRQNGIALKAPITTPVGTGFRSVNVHLRQAFELYACVRPCKSYKG
                     VRSKYENIDLVIVRENTEDLYAGIEFEKGMGETLELIGWLNAHSKRKIGPNAGISIKP
                     ISVEGTERIVTFAFDYARKMGRHKVTSVHKANIMKHTDGLWLDVSRQIAQKYPDIEFE
                     DRIVDNMCMQLVQKPELYDVIVLPNLYGDILSDLCAGLVGGLGVAPGANIGQKGAIFE
                     ATHGSAPKYKGQNKVNPVALILSGVLMLRHMGMDKQADALENAVAAVIEEGKYVTYDM
                     KLDRDDPTAVGTREMADAIIRRINK"
     gene            complement(25973..27634)
                     /locus_tag="PLE88_02550"
     CDS             complement(25973..27634)
                     /locus_tag="PLE88_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HOM75378.1"
                     /translation="MQDYTNVSVLVVGAGPAGLSAAITLKKLLPALDVVVIDKAAGPG
                     NHNLSGAVLESRALGRLLDTAVPNWSDSEEAKAVLLSKVDRDDVMFLLGSKMAFRIEA
                     AIKLAKALKLAFGQMSHKGDYICSVSKLTTWLCKIAAAMGVEILHGFAAEDIIWDTEQ
                     HLALGVKLKDQGLDKHGQKQPNYLPGEVIRAKSILIAEGCDGLLAEKFISKAGLVRQA
                     NQLYSLGVKELIRVSDSQYARFGSGRVVHAMGYPIWTPLIGPAMFGGGIMYPMGGNHI
                     AVGMIVGLDYKYCDFNPQDALTLFKSHSFVRPFIEGGTITEAGVKMIPEGGLYAIPRC
                     PDTQSIGRSNVLLLGDSAGFVNMCKIKGLHNAIESGIAAAAAIAEGLDRPKGLARIYT
                     KQIEEAGVIAEMETAKNFRQTVAKFGPLQGMPLSILGSWLPKFNIEPDYTTMTRATYR
                     LKPAIKYDKDTFTAVAATVHREEQPCHLLIQDETICRNECLGKYGAPCITFCPAGVYE
                     SIHGQPKPANPSNCLHCKTCQRKCPFDNIRWTVPEPSGGPKYKTM"
     gene            complement(27637..29745)
                     /locus_tag="PLE88_02555"
     CDS             complement(27637..29745)
                     /locus_tag="PLE88_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOM75379.1"
                     /translation="MSHYIVLVKQVPDVSQITDNAFDPKTGNLIRTRLPSVINELDAQ
                     ALAFAQHMKHLSGDTLGKIIALTMGPPMAADVLRYALSRCADMAVLLTDRALGGADTW
                     ATANPLAYAVRKICTDLLASSKDYYIVAGMQSVDGDTAQVPAQMAEELGIPCIAYVTG
                     TEYHNGQFQFTRIVSGGSQVVRPRTVPAMITVAKYEYPLFASFERTRKAIAAQLIEWN
                     ANDVKAKAIGVSGSKTRVIRVFPPGKTTRKCQEVQTAQELARIIIENASKDRQNNKTS
                     SDSKPSYLLPAKRENLFDRSYEATEKETEEYQILAAKLKELNITDPTQIDEAAQEKIL
                     SDNRVDFHKKALEDMIAGLTKTQPHYSGEVWVMAEVSEGTLHPAVFELTGKARQLADS
                     LDTYVGVVLAGHEVANLANEIIAAGADKVYLIEHPLLKEFDPRSFCRAVSDAFNTYKP
                     QIFLYAATPQGRVLAPMVSYRVGCGLTADCTGLEIRDSSRKGQIAILMQTRPALGGNV
                     MATICTKDSICQMATARPGVMKRLAPDPSRSGRIIKHNADITDADISLHILRTEKAHG
                     TVRFDSDVIVSGGKGMQNRDNYDRLLDGLCGTIHSRLGISVEKGASRTAVEQGFAERI
                     HQVGQTGTAVGPRIYIAIGISGAIQHMIGVANTETIIAINTDPHAPIFKQCDYYLIGS
                     AEKTVPELINALKDFSNTGQ"
     gene            30101..30727
                     /locus_tag="PLE88_02560"
     CDS             30101..30727
                     /locus_tag="PLE88_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOM75380.1"
                     /translation="MADLKKLSEAIIRGDQRTAVEVTKEALNEGVAPGMILSEGLIAG
                     MNVIGIRFKANEVYSPEVLIAARAMKMAMEVLEPELVKAGVKPLGKAMIGTVQGDLHD
                     IGKNLVCMMLKGAGFEVIDLGVDVSPDKFVEKAKENAPRIVGLSALLTTTMPAMEKTI
                     RAIKDAGLSIKTMIGGAPVTQVYADKIGADGYAADAATAVDIAKRLMN"
     gene            complement(30883..31089)
                     /gene="csrA"
                     /locus_tag="PLE88_02565"
     CDS             complement(30883..31089)
                     /gene="csrA"
                     /locus_tag="PLE88_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009847619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOM75381.1"
                     /translation="MLVLSRQKDESIMIGDDVEITIVDVRGDKVRLGINAPRNITVHR
                     KEIYLAIQKEKEENKHPAPTTGNP"
     gene            complement(31375..32370)
                     /locus_tag="PLE88_02570"
     CDS             complement(31375..32370)
                     /locus_tag="PLE88_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75382.1"
                     /translation="MEWLYPIALRVIAAIALIYFGLGLFNIHWLLRLRILASLSIGAL
                     IVGALGWPLVRPEDPLGAVSLFSMQMTPVQCLILVGLGLTAGAAAAFVCYPLGGNLAP
                     YAAPAGIAVLALNSGAMRQILLTNSALQQRNAMYAFMRWELLFWLGICAAGYLGMLLA
                     LKMLAVKFRNPFSFNTTINWRNWFLAVAAGAVITYLTIGIFAQDINQFDEVLGSVAGQ
                     PGHRQAAFGVFVAVGLAAFIARYFLGTDYIPVILGASLLYIGAFTRIIDSQDLQHLVK
                     TWPIDFYTHTIYAILPIQYAPFSVLGAMTGYWISIQFKHNSSISHTSDKSTLPTD"
     gene            complement(32795..33043)
                     /locus_tag="PLE88_02575"
     CDS             complement(32795..33043)
                     /locus_tag="PLE88_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75383.1"
                     /translation="MTPQEKAQKGLSLLKEAIVEIIESHPNGIRNVDIADLLEIHSDY
                     SGGNKDFLSWSIIGLLLNEGKIERKGKKYYPQKIGVVK"
     gene            complement(33369..33443)
                     /locus_tag="PLE88_02580"
     tRNA            complement(33369..33443)
                     /locus_tag="PLE88_02580"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33407..33409),aa:Cys,seq:gca)
     gene            33907..34839
                     /gene="mdh"
                     /locus_tag="PLE88_02585"
     CDS             33907..34839
                     /gene="mdh"
                     /locus_tag="PLE88_02585"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOM75384.1"
                     /translation="MSKAKVTIVGAGNVGATAAFIAASRALGDVVLLDIVPGLAEGKA
                     LDIAQSKGIFASAGKVTGGTDWDLAADSDIVIITSGLARKPGMSRDDLLAKNAGIVKS
                     VAQQIVRTCPNSFIIVVCNPLDVMTYVAAKVSGFKKSKVIGMAGALDSARFACFAADE
                     LKCDIGDIQGVLMGGHGDDMVPLPRFTSVAGIPLPCLLPQDKIDELISRTRNGGIEIV
                     NLLGFSAYYAPAAGAIRMAEAILNDSKTILSCCAFCDGQYGIHNTYVGVPVKLGAGGV
                     EEIILLDLNEQEKAQLAASAAHVNAMVEKVDTMI"
     gene            complement(34842..36767)
                     /locus_tag="PLE88_02590"
     CDS             complement(34842..36767)
                     /locus_tag="PLE88_02590"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOM75385.1"
                     /translation="MNLFVYGSLRDPSIFESVCGYSFSLKPSEARTGNVLNAELAMLP
                     HYRRVSPDNIYFYAIPDTTSKLQGFVIYDVPASAMKVIDQYEGKFYDRETVQVHTANG
                     TVEAQAYLASTKSMRKRFGDRFHVNLIHELWLRKRIEKFFQSHTRPGEHSIDADIERR
                     ARRQLLGTIERDLVVSHLRQDAVSDFYLEHELNRPCPSMRHLINTPEARPYIENYLAL
                     VIKLAVMNQFESMIYDRFRFELDRLCPSKRYYTRILSLLIALRMINSNSHAIDMILRR
                     GLETMPPGNSYDLIDYVKYAVAAAENAFDPRVVQGDMAIIRNNLQPGLVPMGAEVELS
                     NLGFRAIHKNIANQDPVFDGFRYFCDFAMDVLGWKLGSYVDDHSGEPNRSNRGFLELA
                     PGRLNTLGEISKPATADPWVLNRLIREITEFFPVNPHSLHLSFQLRRNQIGKQSTLPL
                     SFVKCLFVLGGGTQITDTGQIWISRLRNEEIERDDYGEELMFARTSKRRWQLSGDPQE
                     VRPLRQAIYIHQYKFIRLERRASYEPLIMALKGIQLALNPGDYLTAAQLASSQRLRND
                     YEELKEWALQPTPISHSTKHRFLDAIYDGLLHEGHNKPYHKLHYIDWAMGAIDLQIRL
                     FNKKLEEGKAIPSFQSE"
     gene            complement(36830..37978)
                     /gene="alr"
                     /locus_tag="PLE88_02595"
     CDS             complement(36830..37978)
                     /gene="alr"
                     /locus_tag="PLE88_02595"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOM75386.1"
                     /translation="MRVYRTQQFIRPDLQAHIFSQDLLNNVRHLRSLCSPKTKFCAVV
                     KANAYGHGLAEIVNILVRDGADAFAVATFYEALHIASMVFDKSILILEPMNPSQSIEE
                     IQVCARSGFDGVLSSLDSLNYIKEALKKTSLVQKVHINIETGMGRGGIAPEQAGRMLE
                     QVEACPNLKLAGVFTHFATADEDDLSYAYEQIEVFERFLNQYQLRNRNILIHAANSAA
                     VMKLPQAHYDMVRCGIAMYGYYSRRMKNPPIPLQPVLTLQAPITHIAHIPAGHSISYG
                     RSYVTKRDTTAAIIPLGYADGYRRCFSNRAFMKIAHQAVPVIGRVCMDQVILDVTDVP
                     NIQLGQMVTVLDNSHDSVCSAYALADLADTICYEILTLIHAHVNRIVH"
     gene            complement(37975..38712)
                     /locus_tag="PLE88_02600"
     CDS             complement(37975..38712)
                     /locus_tag="PLE88_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM75387.1"
                     /translation="MGDTLEIKKTHPVTSLTVFFPCFNEREAIGPLTVKTLAVLKEIC
                     PDYEIIIVDDGSKDGTGQLADQLAREYPAVRVIHHPVNLGYGAALQSGFRAAAKEYVF
                     YTDGDGQFDVTELHRLLPLMEQYDIVSAYRLKRQEGWKRQLNAFCWTALINFLFNLNL
                     KDIDCAFKLYKRRIFDQIEMISTGALIDTEILARASRLGFRITQVGVHHYPRIGGRST
                     GANFKVILHAFRELFKLRKTILQTGKR"
     gene            complement(38716..41415)
                     /locus_tag="PLE88_02605"
     CDS             complement(38716..41415)
                     /locus_tag="PLE88_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75388.1"
                     /translation="MNELGNTSRRTGWLQLVLFAGMMLFAFYGSTHMVAAGDTWVAMA
                     CGRHFAHHGVDTIEPFSFNSHPAGPSDEQLQKFPAWSRSFIRWWHPTGWINQNWLTHL
                     IYYKLAVWFGSEDKPNYNVLVIWKFALYFLTVFSVYGIGKLLGAGNFLSAAAACMGMV
                     VGRTFFDIRPAGYSNLLVPVYILVLALATYRNWRLIWLMIPLIVFWANVHGGYIYAFI
                     MFVPFIGIHFLLNLPRRWTVSLGCMGLWLVLYLMIYKFLTNDYYVQTQKLLNSDYKAA
                     PFISKMLAMWGILAAGSVILAAIKTVKPAPFYAYHIGVGFIWFLSLCTSFFARIPVNL
                     TPQFKTIYSSFVSSSQLSFCFVFIAGTLLVFAMAVKKERFVILPVRGLIHTIGAAVCS
                     FIAMVILNPFHLTNLTHTFEISVSKHAESWRQVNEWKPAFDWMDKTSTTPNPVGEEEA
                     FAVLCILTGLVLLAWLSGYFLKPQLTIKGSRKSLRQECNLPSGRPRIDLAIIVLSLLT
                     LYMAVQSRRFIALAGSAAAPAVFLLARQAWQMITAKFNLKIFSASDSSNFRIVSAGYI
                     VLSIFLALLGLFWAIKFKRVYLDPWPTDNRYHSIFMRMTASHLKPFEVCQFININNLR
                     GRVFNYWTEGGAIAFGQQPDKDTGQIPLKLFMDGRAQAAYNHDKFRLWQLIHGGGPIA
                     QQALAEGRKLTAEQLREVGLWIDKQLKSYQVWVVLMPQSQEDSTFMQALKLTPNWKTG
                     YLDNTQHLLVDIENPDGRNLIDNILNNKAGFPDSFSQKLTTSVAILENQYAPRFGDLY
                     ELTKGAFDEYPYPAAALALNRLTGLAQFREQATSDLQTYLDDFTQNQEMYKKENGFLL
                     RLGSAEIAARCLMQIKGQEKDRYRQFAEQFRELSKLINTSRIW"
     gene            complement(41416..42603)
                     /locus_tag="PLE88_02610"
     CDS             complement(41416..42603)
                     /locus_tag="PLE88_02610"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOM75389.1"
                     /translation="MKISQRAQSVPPSATLAVTSRAKEMKAQGIDVLGFAAGEPDFDT
                     PEFIKEAAINALKKGQTKYTPAAGMPDLRKAIAEKLKRENNLDYSWEQVVVNLGAKHS
                     VYGAMQAVLDPGDEVLMPAPYWVTYPEAIKLAGAKAVILETTGATNYKITPEQLRKAI
                     TPKTAMLILNSPNNPGGFCYSPDELMALAKVLEGTEVIVLSDEIYEKLVYGKTRFVSF
                     ASLSADAYQRTLTINGLSKAYSMTGWRLGYTAGPLNVIKAMARLQDHSTSNPVTFAQF
                     GAIAALKDQGDTIEKMRIEFEKRGRLMAQRLNAIKGVCCHQPEGAFYCFPDVSAHYGR
                     TIGGAVIRDSMDFAAALLEQAHVGVVPGAPFGSPNNIRLSFACSLEQIEKGMDRIQRW
                     LQE"
     gene            42934..44304
                     /locus_tag="PLE88_02615"
     CDS             42934..44304
                     /locus_tag="PLE88_02615"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HOM75390.1"
                     /translation="MYSKNTRHPVAAVIAAIVFFSLQAAAQESEKTVSSPKELYLPKK
                     ADRVPENNDYNSQDSEYCFKRMVQSDNIAIFWSKEYGDDPLGNPDARKRFDVQFMLSE
                     CERFYKYYIDELKLVQKGNSISDKYKMLVYVFGGEGGTAFGGGVDEKIGVLWTPAVRV
                     NKPPYGVLAHEMVHSFQYIIGIDTGKHLRGSIVEMSAQLMLWQVYPEWMTFENYHLVD
                     FMKKTHYAFLHPTNMYHSPYVLEYWLNKHGKEFFGRLYRETLPNEDPVTTYKRILSVT
                     QDEFNNEMFDASRRFITWDMKRIEKVARPYANQHQCSLIPAEDGWYRIAPSRCPQNYG
                     YNGIRLKVPSPGTEVVLHFNGIAGAEGYNAVKIEMAGWRYGFLASLKDGGRIYSSVGK
                     EPQGILAFTVPENTEYLWLVVMGAPVEHWPVAGGRGRRADNTAEEQWPYQIHLKGTAP
                     DDSVIQ"
     gene            44488..44561
                     /locus_tag="PLE88_02620"
     tRNA            44488..44561
                     /locus_tag="PLE88_02620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44522..44524,aa:Arg,seq:tct)
     gene            44700..45938
                     /locus_tag="PLE88_02625"
     CDS             44700..45938
                     /locus_tag="PLE88_02625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOM75391.1"
                     /translation="MAILVQKFGGTSVANADKIRRAAARALAAHQRGEQVIVVASARG
                     KQTDELIADALEVNPNPPRREMDMLLSTGEQQTVSLFAMALAAMGQKAISFTGQQIQM
                     ITDNSHTKARIRSIGTDRLRKKLNEGYICVVAGFQGVDEDGNITTLGRGGSDTSAVAL
                     AAAMGADYCEIYTDVDGIYSADPRKYPKAVKLEQISYDEILELASLGAGVMHSRSIEF
                     GKKYNVKIHVRSSMNDNPGTLITHEVPQMEGILVSGATSQKDLAKIGLIGVDNKPGNA
                     ARIFAHLAKHNVSVNDIIQTEMSPQKAHLSFTVATSDLSEAKQAIEEIRDEVHCQEVL
                     IREDIAEVSVVGVGMRSHCGVAEKMFKALADKKINIDAITTSEIRISCLVNHNDVDTA
                     LVAVCDAFELDKPVNRRRHP"
     gene            46032..46691
                     /locus_tag="PLE88_02630"
     CDS             46032..46691
                     /locus_tag="PLE88_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOM75392.1"
                     /translation="MAKKKRKPRYAMRRITRNVIAAAFLLIVVSSITLLDRQFGGAIR
                     KTIRQAAYTQPDWQKYHGRQFTVVEVIDGDTLDIDIPDKEFTNTRIRLLGVDTPETKH
                     PTIGVMYYGPEAYNFSKQTALGQRVTILLDTVGDQRDRYGRLLAYVVLPDGRVLNEEI
                     VRLGFGYAHLGFPHTKYAAYEALMEQAMQSKTGLWAGVARDDLPKWLRQKRPELLRNS
                     R"
     gene            complement(46827..47480)
                     /locus_tag="PLE88_02635"
     CDS             complement(46827..47480)
                     /locus_tag="PLE88_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75393.1"
                     /translation="MKSVFVLFMVAALATAVEAADISSLFTNLDFDSGAVTTYPFKGF
                     DAPAATEIIGWSNYPAGSLNDAGVEGPGAWWLTEAYTNVYQNAGFMSAGDAAYNMSTY
                     VIQAGDVFFISYIGGEWGWTGTLGQWTATLFYDNPANVIGSYVQNMNSWMPNAVWYTD
                     AVGIAATPASVGGTLGILFKSTGDRIVQFDEVTVTVIPEPATMVLLGLGGLLLRRRK"
     gene            48568..49128
                     /locus_tag="PLE88_02640"
     CDS             48568..49128
                     /locus_tag="PLE88_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOM75394.1"
                     /translation="MTERKTVLVPIADGTEEIEAVVMIDVLRRSGAEVTVASVDRLNI
                     IASRKVKLTADCLIDDCKNQQFDLIALPGGMPGAEHLRDCAILADMLKQQRATGRLYA
                     AICASPAVVLNAHGLLEGRKATCHPAFREKLPDQSAASRRVAVDANCITSQGPGTAME
                     FAIALVRCLFGDSRAEQVAEPMCFRT"
     gene            complement(49177..50166)
                     /locus_tag="PLE88_02645"
     CDS             complement(49177..50166)
                     /locus_tag="PLE88_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOM75395.1"
                     /translation="MFGDVLSSKRHCFLKGNSMKLATYDYHHAVSCGILTNEGFIDIP
                     SHTQGAKRLHSVKEILIKGQKALEILQELQHSVQERIKPDHIKLRAPIPHPGKVIGLA
                     GNYKKHLEETAWQDGTFDDQAVKTNPWPFLMPASVITGHQTVIPWPVYSQQIDYEIEL
                     AVFIGDNASKITPQQALSYIAGYSIANDISARSVTFKQGRKERPRDVFFDWMMGKWAD
                     GFLPLGPWLVTADEINDPQNLDMELKVNGQTRQKANTSQMIFTIAQIVSFISHLMTLE
                     PGDVIITGTPHGVAAADGSYLHPGDIVECTIQHLGTLTNTIGAKPDNFYRAFI"
     gene            50241..>50536
                     /locus_tag="PLE88_02650"
     CDS             50241..>50536
                     /locus_tag="PLE88_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75396.1"
                     /translation="MFKIFSFRKKKACPQSDILGSEMVSREDFKASYNSLDASVVPLR
                     PAEPVIQKKKDSAEVFNEAVNLLVEKLQSINENLQQQIHQNRQLVERMDALPAV"
BASE COUNT        13541 a        11882 c        11935 g        13178 t
ORIGIN      
        1 aaaaaagaaa acagaacaca agtgaaaaca agggggatgg cagcagcagt tacagcgctg
       61 tgagaatgca gaatcattac aagcagattg aatgccagca gattcaggaa gaattccagc
      121 cccgcaggat agccgaaccg cagcagtttt ttcatcgtgt cggaatccca tctgaaactg
      181 gcggctacgc tgtattcccg acgcacagaa gaacctaaat aaaccgccgc caaaaccatc
      241 aggccgcaga atccgccgat gagagtccca taggccgctc cccgtatgcc cagcggaggg
      301 ataccccatt tgccgaaaat cagcagataa ttggctccta cattgacagc cattgatgtc
      361 agagcagaaa ccattaccac acgggttttg ccgatgccgg agaagaaact gctcaggcag
      421 ttccgcagca gtccgattaa cacgccattg agcagtatat taaagtactg ctgctgatac
      481 atcagctgct cggatgggac ttttataacg tcgaatagcc aaaatgccag cggtcgggct
      541 gccacgatga ctggataggc caacaaggag agtatcaatg cctgtgtgac tgcaacagga
      601 catcgttttt tctgattggc tccccaatac tgggctgcta atgccgtggt atagccggtc
      661 agaccaagaa caaacgtcat cattacaaag ctggtcaaac cgccggccat tgccgcactc
      721 atatattccg gccccagacg cgacaaaaac agccggtcgg taaatgtcat tgttgtttca
      781 caggcgctgg aaatcaccat cggcagggca atggctatca tctccctgat gcctccggga
      841 ccggcaagat ggttatcgag gttgattttt atctgcattg tttttctgcc aaccggaggt
      901 tgctgatagt atctgcataa gcagcgggat tgaatttgcg tttgagtcct gctgcattca
      961 tatagcggac tgtgccgaaa acaggatgcc ggccccatgg ccggtcatgt ttgccgaagc
     1021 accatgcaat tccggcaaag ctgttgggat cacggccatc caattgatat ttattgttca
     1081 ggtgcaaagc gatatgatag gcctcttccg gcgttcggct ccattctatg atttttttgc
     1141 cccagtacat ccgcatatag ttatgcattt tgccggtgag ggccatttca gtttgcgcag
     1201 cattccagaa cggatcgtgt gtccgggcgt gttcaaattc gtcaagcgag tatatagaca
     1261 aacgtttatc tttggcgtgg agctttagtg ttcgcaatgc ccagtgaggc agggcatcga
     1321 actggtcata gcgccgatta taataaacga aattgtggct taattcccgc cgtacaataa
     1381 gctcctcgag aaagctatct ttgcccgggc tgtctgtttt taagatttcc aatgcgatat
     1441 acaggggact gatttgtccg aaatgaagat aagggctcaa atgggactga caattctgca
     1501 gcggatcgct gcgatggtct ggatacgcat ccagtttttc ttggataaat tttctgagcc
     1561 gctgttttgc ctgactggtt ccgccggtgt agaaaatact gggggcaata gaatggtcga
     1621 tattcagctg tttaagcgtt tgctccgtgt tgctgatgtc gtaactgctg aattgcaagt
     1681 tcagtgagct gcatcggggc ttcgaatgct gtaagggaat aaggtattgg ggaagctgct
     1741 tatgaatcct tggccgaaac gttcgagcgg agaagttttc tttatcggat gcttcttcag
     1801 ccggcacaat cagattggca gcaacttcac aggcaagaca atccgcagat agggctatcg
     1861 aatcgcgcca ttgccgctga cagcgcagat acccgtcgtc catcacgagc atatccgcct
     1921 gtcgggccaa ttgggggatg gttttgacag gattgccgcg cagaataaca agctgaatgt
     1981 gtcgctcggc aagggcggct tgtgtttcgc ggagtccctg cagcataaag gcataatgcc
     2041 gctggttggc ttcaggaaag tcatctgtca gtgaaaatgc cgctaaaaca ggttttatcc
     2101 ttttgttggc ttgtaaaatt gcatactcca gcgcatgatt ccattcggcc cgctgcgatg
     2161 cctgcatcca gtacaaaaca aatccgcgct gctggatagg cttgttattt ctgttccgaa
     2221 tgcgctgggc ctgcatcatt gcaatcctcc tccactctta ctgatttttg gattttcggc
     2281 tttcgcggcg gcggtcggcc tctttcagta gccgtttgcg gatacgaatg gatttgggag
     2341 taatttctac caattcatca tcctgaatat attccagggt tgcttccagc gacattttgc
     2401 gggcaggacg gattcgggcg gcatcatcct tgccggttgc ccgcatatta gtcagctgct
     2461 tggcacgcgc cggattgacc ggaatgtcat tgtctttgca gtgttcgccg accacctgac
     2521 cttcatagac catttccccc ggctctacga agaaaatccc ccgatcatac agggcatcca
     2581 gagcataggc ggtaacgata cctgtttctg ttgcaaccat aacaccggcc tgacgctggg
     2641 ggattgatcc gcgcagcggc tcataccgtt cgaatgtgtg gtgcatcaca gcccggcctt
     2701 gggtggcagt cataacccgg gcgtgcagcc cgaaaagccc gcgagaagga atcataaatt
     2761 ccatatggat gtaattatca gcgccggctt tcgtatccat actgattaac tcgctccggc
     2821 ggtcgcccag cagactgatg acagagcttt ggcagtccgc cggacagtca atgaccagcc
     2881 gttcaatagg ttcctgccgc agaccgccga tttcgcggat aatcacactg ggttttccga
     2941 cacacagttc atatccttcc cgtcgcatat tttcaagcag tattcccaga tgcatcaagc
     3001 cgcgtccgga aaccagaaat tcgtcagccc gctgcccggg ttcaacccgc agggcaacat
     3061 tgacctgcag ctctttttcc agccgctccc gaatatgtct gccggttaca tattttcctt
     3121 ctcgaccggc aaaaggtccg tcattgatgc ggaacgtcat attcattgtc ggctcatcga
     3181 cggcgataac cggcaggggg gacggttcat ctgcacaggc aacggtattt ccgatgtcaa
     3241 ccggatcaag tcctgcgatt gcacagatat cgccggcaat aacagtatcc acggtttttt
     3301 tgcccaaacc gtcaaatgtg tacagctgaa gcaccttttg ctgcgtatgt cgtccctgat
     3361 tgtcaataac agtaattttc tgcgactggc tgactgttcc tgcaaaaaca cgcccgatgg
     3421 caatgcgtcc gacataatcc gaatagtcca acgttgttac aagcatctgc aggggggcat
     3481 cggggtcatc cttaggggca ggcacatgct caataattgc atcaaatacc ggctgcaggg
     3541 tggtaccggg ctgggccaga tcccgtgttg cccagccgtt tcggccggaa gcatagagaa
     3601 tcggaaaatc aagtgctctg tcatctgcct tcagggctac gaacagatca aaaacctcat
     3661 tgacaacatc atccggccgc gaatccggac gatctacttt gttgacaaca acaatcggct
     3721 taagcttgtg cagcagtgct ttgcttaaaa cgaatcgtgt ctggggcatt ggtccctcaa
     3781 aggcatcaac cagcagaaga accccatcgg ccatcttaag cacacgttct acttccccgc
     3841 cgaaatcagc atgccccggt gtatcaatga tattgatttt atactcttcg ccatcagggt
     3901 cagtataatg gatagcgcaa tttttactca gtatggttat tccccgctca cgttcaagcg
     3961 gattggagtc cataattaag ccatgctgga cgccggccag tttttccaac tctgcactgc
     4021 gaaacattcc agattgatag agcagctggt caaccagagt ggtcttgccg tggtcaacat
     4081 gtgcaataat agccacattt cgtatatgcg atgtaaatgt cataccattc cttattgtat
     4141 attcaaggtt cctgttcttt tgtgtccaat aaataaaatg caggaacgta tcttatctgc
     4201 gtaaaaaaag caacagaaaa tccctttccc tgcgttaggg attctttcag gcaggatacc
     4261 ctgtaatgca gaaaaaaagc aacaggctat attttaattg ctggagggta ccgataagag
     4321 agaaacgtat attgaacgtc ctttttgagt atattctaat ttgccttccc ttgctaacca
     4381 gccaagtcct tgataagcca tttgagaagt cagatttgtc ctttttggaa tatctgtaat
     4441 atttatcggg ttagctgcct ttgcaagaag atgccaaatt atacccgctg cttgtccaat
     4501 tttttcagtc atttcaaaat acctcacaaa aaagtccttc taaattgcat tcaatcaggc
     4561 aatagtatga actgttgtat ttgtcatgtc aagagtatag ttttattgga acattgggat
     4621 tttatataga attacttgaa taggccctta aagcaggtaa tataacaaat gattcctatt
     4681 cgcatctatg tttccgcaat aaattatatt cgatatacag gaggtaattt tgaaagaaac
     4741 aaaaaaaatc gcctactttt ctatggaaat cggtcttgat ccggctatgc cgacttacag
     4801 cggtggttta ggggttctgg ccggagacac gattttgtca gcagcagata tgaaaatacc
     4861 gatggtggcc gtgacactgc tgcatcgaaa gggatatttt taccagcggc ttggagcaga
     4921 tggatggcag acagaagaac cagttgagtg gattccggag gattttacac gagaactgga
     4981 accgcgtata gttgtgaata ttgaaaaccg gccggtaaaa gtccgcgcat gggagtatat
     5041 tgttaaaggt ttcgggggat atgcagtccc ggtttattta ctcgataccg atttggagga
     5101 aaacgctccg caggacagag ttcttaccca ttatttatac ggcaaagatg cccgctaccg
     5161 tctcagtcag gaaattattt tgggcattgg cggtattcgg atgctccggg cattgggata
     5221 tacagaactt gaccgtttcc atatgaatga aggccatgct agcttattga caatagaact
     5281 tttagacgag cggattcaag cccgcaagga agaccaaata acagaagagg atattgccga
     5341 aatacgacgc caatgcgtct ttacgactca cacgccggta gcggcaggac atgataaatt
     5401 tccgatggat ttggtccgag aagttttcgg cagccataag gcatttatgt tagagaatgt
     5461 tttctgctgt gaaaaccagc tgaatatgac ctatctggcg ctgaatttga gtcactatgt
     5521 caatggtgtt gccaagcagc atggggaagt ctcccgtcag atgttttccg gctataaaat
     5581 tgatgctata accaatggag tcaatgcaac acgctgggtt tgcccatcct ttcagaaatt
     5641 atttgacaag catattcccg gctggcgtga agacaacttc agtcttcgtt acgcgttgag
     5701 tatttcctgc tcggaaatca ctcaggcaca tcagcaggcc aaacgggaat tgattcagta
     5761 tgtcaaccag cagagcaatc tcggaatgga tacagaggtc cttacactgg gttttgcacg
     5821 gcgtgcgacc agttataagc gagccgattt gatttttaca gacctgcagc ggctgcgttc
     5881 gatcagcgta aacgaaggca ggctgcagct tgtattcagc ggcaaagcac atcccaatga
     5941 caccgagggc aaagaactga ttaagcgcat atatagtgct gctcgacagc tgcgtccaga
     6001 aatcaagatt gcatacattc ccaattacga catggatctg gggcttctgc tgacatccgg
     6061 agtcgatgtt tggctcaata ctcctcagcc cccgatggaa gcctccggaa ccagcgggat
     6121 gaaagcggcc ttaaacggcg taccgtctct gagcattttg gacggctggt ggattgaggg
     6181 ctgcatcgag ggaattacag gttgggctat aggagaaaat catcatcttg aacagcacag
     6241 cgacagggct gccgatgcag aacacttata tcagaaactt gaacaagtag tgcgtatcta
     6301 ctataaggag caggcacgtt tttgctctgt ccgaacacat gcgattgcat tgaatggctc
     6361 gttcttcaat acacagcgga tgatgcagca atatgtgctg aaggcctatt ttggctgata
     6421 ttcagcgggc agattccaaa tgttgacgaa tatcacccgg acgtatgcgg ttttcatcgg
     6481 caatacgtcg gatggtgtag tttggggagg cctgaattcc tttctttttg agtgtttcca
     6541 aggccgtatt tatgtcgatg cccaattcct gacaagtctg tatcaaggtt tgttgtccaa
     6601 atccccctga atcggcagca tttgttcctc cgccaaaacc tcgccgctga ccatattgtc
     6661 cttgctggat tgcctgcggc tgctgggatg accacgagct tcgaactttc tgccctaagt
     6721 tgataagcga ggtgaatggt acggtttgtt taatagtccc ccagcataca aggcctgaaa
     6781 caaggaaagc tcctatccat tccaatcgta aatgtcttgc tgcgttgact ttatcaacaa
     6841 aatagcggca tagaggctta atattcaaac aaatatgaca taacgaagcc gctacaaaaa
     6901 cggcactcat acagatatgc agatcattcc attgactctt tgagagtccc caaaatgtcc
     6961 aagaattcat tctgcgtcca gatggggcaa taaacaacag aatgccggac aaggtaataa
     7021 ccaaaaagga aaaagctgtc aaagacgaca caaaaggtcg caaacgaagc gaaaaatgtt
     7081 tttgactcat aaaaagtccc ctataataag ttctatagct gtaatgcagg gaaccttgtt
     7141 ttagttacat atttttatag gggatgttat gcagaatccc gctgtctttc gatatcttcg
     7201 ttttcgtaat ctgcgtcttc atcctcttcg atggtttcaa gggattccat gctgtcttca
     7261 agatcgatat cttccccgac cagttcgtcg gcatcagttt cgacctcttc ttccgattca
     7321 aaatcctcat ctctgtcgat atcgatgggg cgacgcttga aattatggcg tttgataacc
     7381 tgtccgcttt ccagcatttt ggcgtattcg aggctgtaac gggtccaagg tatggctttc
     7441 agccgttcct cttcgatagg aattcctaag ccctcacaaa taccaaaagt cccgtcttta
     7501 atccgtttga gggcttcctg aatttccata agaatcttct tctcttcggc catcaagtcg
     7561 agattaaaat cctgctcata actgtctgtg ccgatatccg ctaagtggac aggcatactc
     7621 gacagctctc ctccattttg gaagatggat tcttccatcg tgtaaacatc cccgctgatt
     7681 tcctgcattt tctcaataag cagctgccgg taaaattcca gctgttttgc ggttaatttt
     7741 ttggatttct ttttagccat cgtgttccct gtcaatggta tatggtttcc agaatacagc
     7801 ttgaattatc gacaatatta aatacccaac ttaacataaa gcgggaatta tagggacata
     7861 tacggttttg gcaacagatt tgctaaaatt tctgtaaata attttaattt ttttaatatc
     7921 tcacaaaaac acacaataat atatccaata agtgtagtat tattgccgaa tacggatcgc
     7981 tttttgttat ttatttttat agaatcccaa aaaggaaatt ggctttgcat cgtataaaag
     8041 acgtgttttt ttctttaatg ggggatagaa aacgtgattc gtttcgagtt gatgactgca
     8101 gcacccaaag actatccttg gtggggtaaa aagaatatga aaaaattatt gcaaatcggt
     8161 tgggtgtttg acagaataaa tttgcagaag caatttttta ttgaatattt agttcagtaa
     8221 aatacaattt tttctttttt gtaaaggaac agcagatata aacgatatag caaggagaac
     8281 tgtgatgcct aacagtatgg ttcccacgcg gatgtttttg acaaagggtg tcgggcggca
     8341 taaatatcag cttaagtcgt ttgaggatgc acttcgtcag gcaggtgtgg ctcagcagaa
     8401 tttggtgcag gtctcatcta ttctgccgcc gcactgcaaa attattcgcc gcgaacaggg
     8461 cattgagctt ttaaagccgg gggccatcag tttttgcgtt atggctcgat gcgataccaa
     8521 tgagcatggc cgtctgattg cttcgtcggt aggggtggct attcccaaaa accgcaaaaa
     8581 gtggggctac ttaagtgaag ttcatggtca tgggatgaaa aagaaagagg cggaagacat
     8641 ggccgaagac ctggcagcag gaatgctcgg cacaactctg ggcatagagc tggacccgga
     8701 tttggcttgg tcagaaaaag aacaggccta caaatccagc ggtttgtttg ttaaaacaac
     8761 taatattacg cagactgcca caggacgaaa aggattgtgg acaactacag ttgcactggc
     8821 tgtttttatt tttgactaaa aatgggcagg gcaagacgca ctcagttcgg tgatttttcg
     8881 ccccgatttt ataatcccga aacggcggcg atagctattc tgcctgttcc ctacgacggg
     8941 acgagcacat ggattaaagg cgcggataaa ggcccgcaag cgatcctcga tgcatcttat
     9001 aatctggaat tttacgacat cgaaaccgat agtgaagttt ttcgtaaagg catttttacc
     9061 gaaccgcctg tcgagggttt tgccgatcca gtgcgtttgg ctggtgtggt gcgtcagcgg
     9121 atgagccgca tactggagca gaacaagttt ccggtcgtac tgggtgggga gcattcagtc
     9181 agcatcggtg ttattgaagc aatggcggac aaatataaag accttacagt gctgcagctg
     9241 gatgcccatt ccgacttgcg tgattcctat gaaggcagta cacacaatca cgcctgtgta
     9301 atggcccgcg ctaaagaatt tgctgctgtt gttcaagtcg gcattcgcag tatggatatt
     9361 tcagaaaaga agaacatgga ttgggggcgg gttttttttt cgcatgatat ctgcagccgg
     9421 actgaccagc aatggattga ccaagccgtg gagcatctga ccgaaaatgt ctatctgacc
     9481 atagatttgg atgtatttga tccgtctatt atgcctgcta cgggtacacc ggagcccggc
     9541 ggtataaact ggtatgaagc tgttcatttg atacgagcgg tctgcagccg cagaaatctg
     9601 acaggatttg atgtcgtaga gctgtgtccc cgtgagcatt tgtgggccag tgattttttg
     9661 gctgccaaac ttgtctacaa aacactgaca tataagtttt gtctttaagg tctataacat
     9721 caactaatta atataacttc cccgcaagaa catatgctga tttttgtccc tgccatatat
     9781 cagtatattt attcctaatt tattggggat aggatgggaa atagaaaaat atccagattg
     9841 tcttgtacca cggtatagaa atacgatata atgggaacag cgagcttaac aagagtgtca
     9901 aaacaccaga atagggagat aggctatgtt tgaaacactg gataagctgt tgctggcggg
     9961 gcttggggca atgtcgatga cgaaggaaaa agccgagcag atttttgatg aatatgtcaa
    10021 aaaagggcag gctcagaagg aacagcgtgc cggctttgtt aaggaggtaa tggaacacgc
    10081 tgaaaaagcc aaacgcgatt tggaaaagat catttctgaa caactggaaa aggcgatgtc
    10141 caagcagccg ctggcaacaa aagaagatat caaacgcctt gaatctaagc tggatcagct
    10201 cttatcaaaa taaggggata aacggtgcga aatccgcttt tcagcgttcg ccggacgttt
    10261 gcgcatatgg tgcgctaccg gcacattatg acagtgctga taaaatacgg attcgaggaa
    10321 attgccggca tttttgcacg gcgtttgaaa atcgggcttg gctcaaaagg attgccgacg
    10381 acgcgaatgc agaatctggc acagacttcg ttggcagcgc gcgttcggct ggcttgtcag
    10441 gaacttggac cgacgtttat caaactgggg cagctgctga gtacccgccc ggaccttttg
    10501 agtgatgaat atattgccga gctggagcgc cttcaggatc aggttgctgc tgaaaagtat
    10561 gaggatatcc ggcttatggt caagcagcag ctggggggat tcccagagga aattttcgaa
    10621 cgttttgatg cagaaccgat agcggctgcc agtattgcac aggtgcatcg ggcacgaacg
    10681 aaagaagggg acgaggtggt tgttaaaatc cgccgcccgg ggatagtgaa aacactgcgg
    10741 accgagatgg agattctggc tgatattgcc ggcattctta aaactcggtt gccaaccccg
    10801 gaaacattta atcctgtgcg aatggtgcag gagttcagcg aggcggtaac aaaagaagtg
    10861 gatttagcca acgaacggcg caaccagcag cgattcatcc ggaattttgc tgacgatcct
    10921 tccgtgcatg tcccccgagt ttttgagaaa tactccagtg aaggtgtatt gacgatggag
    10981 tacattgagg gcattaagcc caatcggatt gatcagattc ttgcagcagg attgaatcct
    11041 aaaattattg cagcgcgagg agcagatttt gtgctgcgtc aggtgtttga gtttggtttc
    11101 ttccatacag atccgcatcc gggcaatttc ctgattcttg acgggaatgt gttggcgccg
    11161 ctggatttcg ggcaagtcgg gcgattaacc aagcaggacc gtatgctgct gcaatatctg
    11221 gttttggcgg ttgtcgaagg ggatgtgtct cggattgtgc atggactgga acgtgccgaa
    11281 atgctggatg aggaaacaga tcttgcagaa ttaagcaggg attttgagga gattcttgag
    11341 acgtactcca accttccgct gaaggatatt ccgtttggcg aggctattcg gcgaatgttt
    11401 gatgttatcc gacaccaccg tattgagccg ccgcgcgatt ttacgctgat gctgaaatgt
    11461 ctgatgacga ttgaatcttt tgccaacgac cttgacagca attttcaaat tatggagcat
    11521 ctgaagccct atgccagacg ctttacgctt cagcagataa gtcccgaagc actttttcga
    11581 caggcccgga cagcggcccg gagtgccagt gaactggctt ctcggctgcc cgaagatgca
    11641 gccgctatca tcagcaagtt tcgccggggc aagtttaaga ttcacgttta tcacgaccat
    11701 ctcgaagaaa tggaacagac gctggacaac agctccaatc ggatttcgtt tgcggtgatt
    11761 atcgccgcat tgctgattgc ctcgagtatg ttggtcccgc agagcggcgg tgtgctggga
    11821 attgttacat ttcagacact cggcattttg gggtatctta ccgcaatggt gatgggattg
    11881 tggctgcttt tttctatcat ccgccaccgc cgattctaag caattgtgcc gtttaacgaa
    11941 cgaataaata tttgaattct gtgccggatt gtctataatc aggcagccga taaaatacag
    12001 caatcttatg ccgaggaaga aaaatgatgg atatgcacaa agaatatcaa aggcgcataa
    12061 gcaaggtgcg ccagcagctt cgggatgcaa aagcggatag ttttatcgcc acggcagtgg
    12121 aaaatgtccg ctatctgaca ggatttcgcg ggcatgacag ctgggcagtg attttgccga
    12181 aagccgttat tctgattacc gacagccgct ataccgagca ggcccgcggg gaatgtgtcg
    12241 gctgccggat tattgagcgc aaggatggtc ttgtcaaaac agctgccgca ctgctcgaaa
    12301 agcagtccca tattgaaaca gttggcattg aagatacctg cccgctgtcg ctatttaaaa
    12361 cactgcgaaa agagctgcct gtacaagtga tgccgacgaa aaatgttata gaaaatgtgc
    12421 ggcttctcaa aacagatgga gaaatccagc ttcttcgaaa ggccagccgg attgcttttg
    12481 atgctatgga ttgggcactg gggctgctga tgccgggaat gacggaacgt cagcttacag
    12541 cattatatga atatcgggta agtgaataca aagcaaaagt cggctttgaa acaattatct
    12601 gttttggccc aaacggctca cgcaatcacc accagccggg ggaacgaaag cttaggaaaa
    12661 atgacactat actgattgat ttcggcgtca attatgaagg ctatatcagc gatatgacac
    12721 gaagttatgc gtttggcaaa gtatcgaatt tttaccgcaa ggtttatcaa acggtagcca
    12781 aagcacaggc tgcggcgatt gaaacaatat gtgcaggcgt caaaatggct gatgtggatg
    12841 cggccgcccg gaaggtcatt gaagattcgg gtttgccggt gtatggacac gggaccggcc
    12901 atgggattgg tctgcaggtg catgagaatc cttatttgac caaaacagat aaaaaaggac
    12961 gcttgcaggc agggcaggtc attacggttg agcctggcat ttatctgccg gggcaattag
    13021 gtgtgcggct ggaagatgat gtgcttgtca ctgaaacagg cggggaagtc atcagtcggg
    13081 atgagcggtt tgacatccat atagataaag ttcctcagct tgggtaatat cgcagaaaga
    13141 ggacttaatt ggggataatt tatatcttta tgggaaaatt gttcttgttt tcatattttt
    13201 ctgtaaattt tcttgcaaaa tacagccgat tgtagatatt acgctgacta aaaaaagaat
    13261 tcttttgaga gttttttatg aaaacaagac tatttatcgg cacagcagta ttaatcgtta
    13321 tgatgcttat agcaggatgc aaatgcccct gcaaaagcac ttcatcatgt cctattccgc
    13381 acaaagtagc ttcagcagaa ttatcctcac ctaatcaggt tgattagccc ctgtttatta
    13441 agacaaaagt atggcttatt cagtcatact tttgtcttgt tgtgttaaac aaagaattat
    13501 cggaccaagg agggctggtt cagttcgtcg tcaatccaat atttattgcc tgtgattgtg
    13561 gcttgaagca taagcccatt ttcggtaatg gtataaacgc tgatttgatt acggagagtg
    13621 ccttcgccgc cggcttcacc gccagtttct cctatttttg cagccgcatc tgcctgcccg
    13681 ccaacatccc agccggtacc aacaaatttt tgcatggcat cccggctttt gaaaactaag
    13741 atctggcgat aatctttagc accaagcccc agaccaagac ctcccatccc catccgcata
    13801 taggttttaa caccggtggt cttatctaca acaaccccat agccggctcc gccgctggca
    13861 aaaagtacag ttacattggc attgctgaag actgcgtaac cggctgcttg ggctacttca
    13921 tcccgggctg agggctttat ggtataaaga tgctgcaaac ttgaagcggc catatcgtca
    13981 ataatccgac ggcgctcgga cagtgtatac ttgctgctgc tgggaccgca accggttaaa
    14041 acggcaaaac ttgtcagaac tattatgata atgatgtgtc ttctcattag aagaatcctt
    14101 tctgctgatg acagagtatc taattcagct ctttatcttt ccaatatttt gtgcctgtta
    14161 cagtggcctg caatgctaaa ccggcttcgg tcattgaata aaccttgacc gcgccaatca
    14221 tgccttcggc actggcttca gcccctttat cgggtgtttt agcagcagca tccgcatggc
    14281 cgccgaactc ccagccgctt tcaatgaatt tttttatagt ttcctgcgta tcaaaaatca
    14341 gaacctgccg gaaatctttg gcacccaatc caagtccaag cccgcccatt cccatacgca
    14401 tataggtttt agttccggcg gttttatcga caaccacacc atatccgccg ccagcgctgg
    14461 cgaagataat attgacattg gcattgctga aagcgccata gccggcggcc ttttcaattt
    14521 cttctcgtac ggaggccttc tcatggtaga ggcgttcgag cgtttctgca gccatcgcat
    14581 cgatagcact gcgtttttct tcgtttgtga gttgattgct gggaccgcaa ccgactaata
    14641 gagtcaagaa agacatccat agaagtgaag aacgtttcat tctcgctgct cctgtttttg
    14701 tgtcattcga atgcttatgt gtccacaatc aatcttctac attacaggaa agctcgaaaa
    14761 aatcaatagg aatgtatttc tattttcccg accgatgtag ttttgcataa ggcgtaggat
    14821 ttcagaaaaa aagcctttgt ttacagattt tcagattact taatacagtt ttgtttttgg
    14881 caaggtttta cggctaatcg ttaaccagcg ggaaaaacgg ataattcaga cagaaataca
    14941 tgacaaatta tctttggaat catgcagaat agaatacaag agacctcaag tcctcttgtg
    15001 ttctgtaaag gccaatcccg agctgttttt ttcataacac acattcaagg taagatgctt
    15061 taatcgagtt atttattctg tcacccacag gtttggcttg tatcggcttg cagaaccctt
    15121 ataattttat ttatggacaa cggtcaagca aatctgaaag atgcggttct ctgggaaaaa
    15181 aaggacggta aagtgcagtg tcttctgtgc agctggaaat gcctgatatc caaaggtgct
    15241 gtaggtcact gcaaggtacg caaaaatatt aacggtcatc tttataccct taattatgat
    15301 aaactttgtg ctgctaatgc tgatcctata gagaaaaaac ctttatttca ttttcagccg
    15361 ggctctcgga gtttttctat tgcagccccc ggctgcaatt tccaatgtgt tttttgccaa
    15421 aattggcaga tcagtcagat gccgtatcag aatattctgg aaggcacatt tttttcgcct
    15481 cggaaaattg tagaagcagc gctggatgat aggtgcagga gcattgccta tacatatacg
    15541 gagccgacag tttttatgga attgtgctgt cagactgccc gtttggcaaa agccaagggg
    15601 ctggccaatg tatttgtcag caacggtttt atgagtcagg aagccgtgga ctttgtcaaa
    15661 ccttggctgg acggtattaa tattgacctg aaagcctttt ctgacgattt ttaccgtgat
    15721 atgtgcaagg cccgtctgca gccggtcttg gacaccatcc gctatattgc tcatcatact
    15781 gatatttgga tggaaattac caccttgata attcccggca aaaatgattc cgacgaagaa
    15841 ttgcggtcca ttgcacagtt tattgtgcaa gaggcctcgg cggatgttcc gtggcatatc
    15901 agccgcttct ggccgcaata taaactgacc aacgtgccgc cgaccgacag cacagttctg
    15961 gaccgagcct atgatatagg cagacaggcg gggctgcggt atgtctatgt tggcaatatg
    16021 cccggtgctc gtgccgaatc aacattctgc tacagctgcg gcacacttct gattgaacgg
    16081 cagggttatc gaattatcca gaaccgcatc acggacagcc gctgtcctgt ctgcggtgct
    16141 gatatcgccg gttttgaaat aagtccctaa acttccttgc ctgctggacg tagtaatgga
    16201 aaaaggtatt tggaaaggag gcctttatgg caaacttgct gtatattcag gcatcacctc
    16261 gccaagagcg ttcaaaatca aatcaggttg cttctgcttt tctccgacac tatgccaaag
    16321 ttcatccgca agacaaaata ggcactgtta atgtgtttca agaaaagctg ccgactttcg
    16381 acggattaac tgttcaagcc aaatatgcca ttctgcatgg ccagccgcac acagatgcag
    16441 aacaaaccgc ttggagcgct gttgaaacag tgattcggca attcaaacag gcagacaagt
    16501 atgttttttc tcttcctatg tggaatttcg gcattcccta tcgcctcaaa cactatatcg
    16561 acattgtcgt tcagccgggc tatacttttt ctgccggacc aaacggctat gaggggctta
    16621 ttcgagacaa accggctgcg gttatttatg cccgcggcgg cgcttaccgg cctggaaccg
    16681 atgtacaggc gtttgatttg cagaaaaaat atatggactt aatactgggc tttatgggtt
    16741 ttgagaagat ctattcaatc gtaattgaac ccaccttgca cggcagccct gatgaagttc
    16801 agaaaattgt tgaatccgcc gtagaacagg catgccggct ggccgaagtg ttttaaatct
    16861 gttttgactg aaaaattttc gattcagtgc cactgagcag ccgccgtata tttgcccgat
    16921 gccgaaaaac caccagcagc gacattaaaa ctgctccggc cagcagcggc cacaatgtcg
    16981 ccagccccca gtctttgaaa agagaagttg ctattgttaa aaaaattgga aaactgacag
    17041 ctgctaaaat ggaagccagc gaaacatacc gccagaggag aacacataca atccagactg
    17101 caaatgcagc aatcccgcag aaagtataat aaggccataa acccagcaca atgcccagac
    17161 tagtcgcaac ccccttgcct cccttaaatt tcaaatagac agggaaaata tgccctaaaa
    17221 cggctgcaca tccgaccagc agccataagg tcagttcata cgctttcggc tgggggccta
    17281 cgagacccag cacgggcacc gcaagcatcg gaatcaaacc cttcagcata tccagtataa
    17341 agcacagtat accccacgac cggcctaatg cccggaccac atttgtggct ccaatgttgc
    17401 ctgaaccgat gcagcgcaga tccttgccgt gggcctttgc tatcagcaaa ccaaacggta
    17461 cggcacccag aaaataggca gccattatca gtgttatcaa ttccatcatt actgacattt
    17521 tttttccgca agaatatttc tgtacagtgc tttaagaaga tttacgtgtt gttcgatgga
    17581 taccgcaacg cggagattat catccagaat cgccttacgg gcaagattta ttttctccat
    17641 gctgcagaaa tactgcagcg cataagtcag cgtgtgaaca tcatcggggc gatccagaat
    17701 aaacccatgc cgcagatgag tatagcgttc ggatgcaccg ttgaaccgtg ttgttatgac
    17761 aggttttgct aaagccagag cctccagaac aaagcgggag cagggatcat aaaaggtcgg
    17821 cagcacggcg gcatcgcagg ccgatatgat cgcattcata cagctatgcg tcccaaggaa
    17881 aacggtattg ttgggggctg aataagctgt cggcgaaaag ccagccgcag ccagcacagg
    17941 atgcacatca gacggcggat tctcccgaag catccgcatg gcggccagaa ggggaccaat
    18001 gcctttcagc cgaggattgt gcgcagcaaa caaaaataag atgggcgttt tgccagccgg
    18061 cacaggaacg ctctgcaaaa tcgaatccct tattttttgc gcttctgact ggtctaacgt
    18121 ctgcggcaaa ctgataccgt tggggataac agttattcgg ctgtccggca gagtgtagtg
    18181 tttttggaac tgcgatttga cataatgact gagagccgca ataatagttt ggttatgctg
    18241 acgacatagc ctttcctccg cctttaaata ttgtgttcgc ctgtaattca gtaaatgcgt
    18301 ggagcgtttc aggagacgct gcatgggatt cgcatacgat gctgtgttgc gcagcattgc
    18361 ttcacggtag ctgccgccgc gcggctgata aatatcagca actgctgttg gcagggtgct
    18421 gtgaatgata tcataaaaat ggttttctaa gtgagcttgc agggcggctt caaatatccg
    18481 caggggaacc ctcctgccgg ggtaatcttg acagaggatg tgcaggtatt cacaaggagt
    18541 tcccttggca gccaaaatga cagcttctat tccctgacgc cgcagttcgc ctaccagttc
    18601 gctgacggat cgttctgcac cgccgagctt aacttctgcg cgttcaatga gaattgccgc
    18661 ttttatcatt gttctatgca tccttttcct gaaacggaca gatgcgccca tgcttgcgat
    18721 tgtgtcgggc catgcaaaga gctttggaat gcacctgccg gaggagttct ttgtcctgcg
    18781 cggttagttt ttccctctgg acgtaagccc gaaaaaaacg cagccggtcg ctgcggctga
    18841 tttttgcagc agggctggaa taatgcagtt gagcaatgtc tttgacctga taatagcggc
    18901 tccgcattcg aggtttgaat gtccgatgca gatcaatcaa gtacagtatt tgttcatccg
    18961 aaagaaacag atgagacaaa tacaaatctt gatgccgata ccctgtggca tgaaagcggc
    19021 ggacaaaatc agcaagacgg cagatacatt cattctttgc ctgccgctgc atgggagccg
    19081 gactcgtttc aaagcaatcg gggagcttct gctccagcga ccggccatgg ggtatttcca
    19141 gtgtcatgat aaagctgcac ttctccgacc acccgttcca ccgtccgccg taggcaatca
    19201 cctgcggggt tagaaccgct gctcgggtca gttcctcaga aggcccttta tcgtattcgg
    19261 ccaagagcgc tcgatgccgg tgttgtatcc atgctttcag ctgaacaaaa aagggaggac
    19321 taatgtatcg cttcagatag gccgtctgcc cgttggcaag ctgaagacga atgcggtgtc
    19381 tccatgcggc caggctcgat ttggatagca tttgtccctt tttgcagcaa atcaccgtct
    19441 ccagagaatc cagtccggtt tcagccagaa tccggctata ggatggcaga acataaaatc
    19501 ccggttcagg ttgtataaaa ggagatgtca tggatgtttc agaaatcggc acgccgcctc
    19561 aaaaacctca tccactgtaa tagactccat acataaatgc tttttcatca aacaacgcgg
    19621 cctgccgcat ggtacgcagg gtgcccgtcc gacgatttga atttccttat tatgccccgt
    19681 ctgtgtccac tgcggattat tcggcccgaa tagagaaatg acattcttgt tcaacgctat
    19741 agcaatatgt cgcggacctg tatcattggc aatcacaagg tctgccagag aatacagtgc
    19801 cttcagctgc ccgccggaca gcggcgtctt tgccagattc agcatatgac atgccgcttg
    19861 tctgctgatc gcttctgcca tttcggtttc ttcctttgca ggagacaccg aaagaagtac
    19921 agtcgcatgg aatgctgtat aaagcttatc cgccagcttt gcatagctgg ccgccggcca
    19981 ctgtttactg ggaccgaagg ctccgcccgg cacaaaaatc acaaacggcc catcaatgtt
    20041 ttttagggcg ggaattttct gataaacctc tgcaatggca ctgtcttcaa cagatagttc
    20101 cggcgggctg acggcttgcc tgcttcccag catagtcgcg atggaatgat aatattctat
    20161 cataggaata ggcttgaata caccgttttc tcggggcggc agcagccgat ccgtcagaag
    20221 aagccagcgt ccttcccgca catatccaat ccgatgcttg atgccggcca tggctgtgca
    20281 aagtgccgaa ccgaatgaat tcttcagcaa tatagcagca tcaaactgct gctgccggag
    20341 aactttgatc tgacgaacaa aatgcctgtc tatagtgagc cattcatcgc agaatgggct
    20401 tggcgataga atttcccgcg taaagctaga tgccaaaaaa cagatgcggg catccttaaa
    20461 taattcacga aaagcccgca gagccggtgt tgccataatg gcatcgccca gcggtgaggg
    20521 cagccagatt aaaaccttcg acggatgcat tgctgttttc cccgcccggc taatcccgcc
    20581 catgcatcat cagcagggca ataaccagca gctcaagcat gatagcatat ccaatcataa
    20641 tcaaaaccgg ttcatagccc actttgggat taagccagaa tccgatactg acaatcaggc
    20701 agaaaacagc aaccgtttga gtcattccgg ccagcagctt gaataccgaa aactccggat
    20761 aaaatcccgc ccgatccatc cgcttgagat ggcgaagaat ttcctcaaac agccgatgcg
    20821 tctgtccggc atcttccggc tggtcattct ctgctgcttt ggtggaaaat tcccttgact
    20881 caaaaccgct ggtttcatgc agggtgataa cctcatccga tgaaagcgac tttgctccct
    20941 ccaatccggg gtcacttgcc tgctggggag gctgggcgtc tgtctcttgg ctttctttcg
    21001 gctctgggac ggccgcccgt tgagccgttt cggtgaaagc tgacgactcc ccgttacgcc
    21061 ccgaccgtgc cttttgatga aattcatgca tccggataat attgacggct tcccgaatat
    21121 taacggcttt tcggtccggt tccggatcgt cgggcagctt ctcccgctgc ttgccgggaa
    21181 catcgacaag aatcgtatag catcctgcag cccgacccat tcgaatatcc agatagcggt
    21241 ctccgacaac ccatgaacgg cttaaatcga tgtctttttc ccgagctgcc tgcagcaaca
    21301 tccccggttc gggcttgcgc agattgcttt ctttggcata ttgggcaacg gttccttcgg
    21361 gatgatacgg gcagtaatag atcccatcca aataagcacc ctcagcagcc agcaggtttt
    21421 tcagccgccg atgaatctct tccagttcct gctccgtaat cattccccgt gctacagcgg
    21481 actggttggt aacgactatc agcagatacc ccattttttt cagctgaatc agcgaccgtg
    21541 caacccctgg cagcagtttg acctgatccg gcgagcggat ataacccggg tcttctatca
    21601 gggtatcatc acggtcaaaa aaaacggcct tatgccccat aatcctgatt ccgttccaat
    21661 tcttttaatt tctcaatcgt cgctgtagag cttttaccgt ctaccagagg tgccaagact
    21721 acacgccccc cgcaggcctc gacaaattct ctccccacaa ctcctttttg ggcccagtca
    21781 gcccccttaa ccagcacgtc cggccgaacc tgttttatca attccagcgg attcagctgg
    21841 tcgaaaatgg taacataatc gacgctttcc agagctgcca gaaccgcggc acggtcatgc
    21901 tgattattga caggccgccc cgcacctttc agctgccgca ccgaagcatc gctgttaagc
    21961 cccacaacca caatatgtcc ctgctgcttg cagaatttca gatattcaat atgcccccgg
    22021 tgcagcacat caaagcagcc gtttgtaaag acgatagttt ctttctgctg acgatgatac
    22081 gccagctgct ttatcaaatc ttcaactgta tgaattttcc ccatcttccc gcggtggcgg
    22141 gaaatgattt cattgacaat ctcatcgacg ctgacggttg caacgccgaa cttttccact
    22201 tccagtcctg ccgcgatatt ggccagctgc agggccgtaa ctgcatcgca tccggctgcc
    22261 agagcaacag ccagcatcgc cagcatcata tccccagctc cggttacatc ataaaccatc
    22321 cgcggcaggg tcggaaaatg ctgcgccagt gtttgtgtcc gaaggtaggc gccgtcttta
    22381 tccatagtaa taacagccat ttctaaatcg agctgctctc gaagcattag cgacgctctg
    22441 gccgcatcat ccggggatct gatttcgaat ccaaccgcag cacaggtttc cttgcggttt
    22501 ggagtaatca tgctggctcc cctgaatttg ctgtagtctg tatccagagg aggatccacg
    22561 agaacacgtt tgccggcctg tctggccaga gcaatcagct gctggcagaa atccttttcc
    22621 agcatgcctt tgttataatc ctgcaggcat acaatattgc aatgaggcag ccggctttcg
    22681 taaacactca gcaactgctg atagatattc gccggcagcg gcccgatgca ttcctcatcg
    22741 atccgcagaa gctgctgccg atgacggtgc tgggccaaac cgataattcg ttcttttgta
    22801 atggtagggc ggctgtccag agcaatcaat ccctcggtgt cggcgcccaa ctgcttgagt
    22861 atttcaagca gacgctcgcc gctgcggtct ttgccaattg ctccgatgca gaagcatttt
    22921 gctcccagag ttgtaatatc ggccgctacg gacgaagcac cgccgccggc atattcccgc
    22981 cgaacgactt tgagaacctg caccggagct tccgggctga tgcgaagcgc atccccgtaa
    23041 acatagctgt ccagcataaa atcgcctacc agcagcacgc ggggacagcc aagattggta
    23101 accgctttca ataaattcgt ataaatcatg gttttgctgt ttgatttaaa ttaccgatcg
    23161 gcagattatc aactaaacct gttattgtat caagtccgtc ccaaaattcc agttccatta
    23221 ccaaataggc aaaaatccgg tcttttcgat ccggcgcaag caacgcactc ttaacccagt
    23281 ctttctgggt acagacaata gcctcagctc cgcaggcatc tgcccggctg aagatctgct
    23341 tcaagtcctg ggttgtataa agatgatggt cattaaacgc tattgtttcc actgtatcga
    23401 tgccatttct ccgcagacgg tcaaaaaagg cgttgggatt gccgatgccg cagaaagcca
    23461 gcaccggttt tccccgcagc tggtccaatt cgatagccgc attggaatag gttgccgcgt
    23521 gcgtgtgccg gtgcgctgct ttagcaatcg gaatgcccgg cgcataacgg tgtatctcct
    23581 gttcaattcg gcctatctga gcttcatcgg cttggtcaaa ccgtgtaatc accgcggctg
    23641 atgcccgctt taagcacgat ttcggctccc gcagaagtcc tgcaggcaat acacgattat
    23701 acccaaaagg acacgtcgca tctatggcaa caatatccaa atcccgcttc agtttgcggt
    23761 gctgaaatcc gtcgtctaac accaaaacct gtgcctgatg ctgttcaatc gctttgcgag
    23821 cccctgaaat ccgatcagca ttgactacca ccgccgtacc gggacaggct tttgtcagca
    23881 gggcaggttc atccgaaatc tgttcaggct gggttttata cccgcgggtc agaattgcgc
    23941 agcgaatgcc tttttggtgc agataattgc acatccatat caccagaggc gtttttcctg
    24001 tgccgccggt tgtaatattg ccaatgctga tgaccggaac ttcgactgag caggatgaca
    24061 gaataccaag atcatacatc aggttacgaa tccgcattcc aagaccgtat agaatacctg
    24121 ccggacgcag tataattcgc aaaaaagagg cccggaatcg cttatcagcc cccgagatta
    24181 tgctgcgata agtattctgc ctcaaatagg tgttccttat aagacctgtt aattaagagg
    24241 ggaaccgtca atcttcctga aaatcgcgca agcaattctc atttattgat ccgcctgatg
    24301 attgcatccg ccatttcacg ggttcccact gccgtgggat cgtcacggtc cagtttcata
    24361 tcataagtga catatttccc ttcttcaatt accgctgcaa cggcattttc cagtgcatcg
    24421 gcctgtttgt ccattcccat atgccgcagc atcagaacac cgctcaaaat cagggcaaca
    24481 ggattaacct tgttttgacc cttatacttg ggggcgcttc cgtgagtggc ttcgaaaatt
    24541 gctccctttt ggccaatatt agctcccggc gctaccccga gcccgccgac taaacctgca
    24601 cacaagtcac tcaatatatc accgtacaaa ttcggcagca caataacatc ataaagttcc
    24661 ggcttctgga caagctgcat acacatattg tccacaatgc ggtcttcaaa ctcaatatcg
    24721 ggatattttt gcgcaatctg ccggctgaca tccagccaca gaccatctgt atgtttcata
    24781 atattggcct tatgcacact ggtcactttg tgccggccca ttttgcgggc atagtcaaat
    24841 gcaaatgtta caatccgttc cgttccttct actgatatcg gcttgatgct gatgcctgca
    24901 ttgggaccga tttttcgctt gctgtgggca ttcagccagc caatgagttc aagcgtttcc
    24961 cccatcccct tttcaaactc aatgccggca tacaaatcct cagtattttc acggacaatt
    25021 accaaatcaa tattctcgta tttgctccgc acacctttgt agctcttgca tggccgaaca
    25081 catgcataaa gttcaaacgc ctgacgcaaa tgcacattga cgctgcggaa acccgtccct
    25141 accggtgtcg taatcggcgc cttcagggca atcccgtttt ggcggatgga ttcaagcgtg
    25201 gcttcaggga gaggggttcc ggccgtttcc attatatcaa cgcctgcctg ctgaatatcc
    25261 cactcaattc cggcccccgt tgcatcaata caccggcgag cggcatcagc tatctccggc
    25321 ccgattccat cgcccataat cagcgttact tttgtcatta acttcttctc ctgtaaagaa
    25381 ttatctgtta atccgttcag agagtctact gtattgagta aattttagta ttggttttgc
    25441 gagtttcaca aaggttaata tcctacttgt aatactattt aatgtcaaac gaaaacacag
    25501 cagaatcccg tataaatgga tttacatttg cagaaatatt tcttccgaat gaatagaatt
    25561 accagcattg tcaatcccgg cgtaacatta gctataaccc gtattctgaa atcgcatcag
    25621 taatcacttt gaaccggatg tataatacca acaaaataca gtaaccactg ctgtcccaaa
    25681 tttctacata aacagtatcc aattttctga gtttcgaaaa aagaagccag caactggaga
    25741 agttgatgtc aactttgggg gcactctgac actggctggg acaagcttcg aatctccgca
    25801 atgccaaata agtgttttgg atgcgaaaag caaattttca aaatactgaa tgacaacaga
    25861 aatacaaata gaaaatcaaa tataaaaata aacagactat ctccacaatt acagccaaat
    25921 attttgcaag tattccaaaa ctgacaattg tttaatcaga attattgatt ttttacatag
    25981 ttttgtattt tggacctcca gaaggttccg gtacagtcca tcggatatta tcgaagggac
    26041 acttgcgctg gcaggttttg caatgcagac agttcgaagg attggccggc ttgggctggc
    26101 cgtgaattga ttcataaaca cccgctggac agaatgtaat gcacggggcc ccgtacttac
    26161 cgagacattc gttcctgcat attgtttcat cctgaatgag caaatggcag ggctgctctt
    26221 cccggtggac tgttgcggcc accgcagtaa atgtatcctt atcatatttg attgccggtt
    26281 ttagccgata agttgctctt gtcatcgttg tgtaatccgg ctctatattg aatttcggca
    26341 gccaactgcc cagtatcgaa agcggcattc cctgaagcgg tccgaatttc gcaactgttt
    26401 gtcggaaatt ctttgccgtt tccatctcgg caataacgcc tgcctcctca atctgtttgg
    26461 tataaatccg ggctagtcct ttgggtctat ccagtccctc tgcaattgca gcagctgcag
    26521 caatgcctga ttcgatggcg ttatgaagcc ccttaatttt gcacatattg acgaaacctg
    26581 cactgtctcc cagcagtaat acattgctgc gtccaataga ctgagtatcc ggacaccgcg
    26641 gaatagcgta taggccgcct tcgggtatca tcttgacgcc ggcttcggta atcgttccgc
    26701 cctcgataaa cggacgaaca aaagagtgcg acttaaagag cgtcaaggca tcctgcggat
    26761 tgaaatcgca atatttgtag tcaagcccta caatcatacc tacggcgatg tgatttccgc
    26821 ccattgggta cataatgcca ccgccgaaca ttgccggccc aatcagcggc gtccagatag
    26881 gatagcccat tgcgtggacc actcggccgg agccgaaccg ggcatattga ctgtcgctga
    26941 cacgaatcag ctctttgacg cccaaagagt agagctgatt tgcttgacgg accaaaccgg
    27001 ctttgctgat aaatttttct gctaaaagac cgtcgcagcc ttcagcgatg agaatacttt
    27061 tggcacggat gacctcgcct ggcagataat tgggctgttt ttgtccgtgt ttgtccagcc
    27121 cctgatcttt gagcttgaca cctaaggcta aatgctgttc ggtatcccaa ataatatcct
    27181 cggcagcaaa accatggaga atctcgactc ccattgctgc ggctattttg cacagccaag
    27241 tggttaattt gctgactgaa cagatgtagt cgcctttatg ggacatctgt ccgaaggcaa
    27301 gcttgagtgc tttggccagt ttgattgcag cttcgatccg gaatgccatt ttgctgccga
    27361 gcaaaaacat tacatcatct ctgtccactt ttgacagcag aacggccttc gcttcttctg
    27421 aatcgctcca gttgggtact gctgtatcca gcagccggcc gagcgcccgg ctttctaaaa
    27481 ctgcgccgga aagattgtga ttgccggggc ctgcagcctt atcgataaca actacatcca
    27541 gagcgggcag aagttttttg agtgtaattg cagcagacaa gccggcaggg cctgctccga
    27601 caaccaaaac agatacattt gtatagtcct gcatttttat tgtcctgtat tcgagaaatc
    27661 ctttaatgca tttataagtt ccggtacggt tttttcggcg ctgccgataa ggtaataatc
    27721 acactgctta aatataggag cgtgcgggtc ggtattgatg gcaataattg tttcggtgtt
    27781 ggctactcca atcatatgct ggatggcgcc tgaaatgccg attgcaatat agatccgggg
    27841 ccctacagcg gttccggtct ggcctacctg atgaatccgc tctgcaaaac cctgttcgac
    27901 agctgtgcgg gaggcgcctt tttcaacaga aatccccagc cggctgtgaa ttgttccaca
    27961 gagaccatcg agcagtcggt cataattgtc gcggttctgc attcctttgc ctccgctgac
    28021 aataacatcg ctgtcaaacc ggacagtgcc gtgggccttt tctgtccgca gaatatgcaa
    28081 actgatatcg gcatctgtaa tatctgcatt gtgtttgata atccggccgg aacgggaagg
    28141 gtcaggggca agacgcttca ttactcccgg acgggcggtt gccatctggc agatagaatc
    28201 ttttgtgcag atggtggcca taacattccc ccccaaggca ggacgtgtct gcatcaaaat
    28261 cgcaatttgc cctttgcgcg agctatcgcg aatctccaga ccggtacagt cagcagttag
    28321 gccgcagccg acgcgataag acaccattgg ggcaagaaca cgcccctgcg gagttgctgc
    28381 ataaagaaaa atctggggct tgtaggtgtt aaatgcatcc gaaaccgccc tgcagaaaga
    28441 gcggggatca aattctttaa gcaggggatg ttcaatcaga tagaccttgt cggcaccggc
    28501 ggcaataatc tcatttgcca gattggcaac ctcatggcct gcaaggacaa cacccacata
    28561 ggtatccaat gagtcggcta actggcgtgc tttgccggtt agctcaaaga ccgccggatg
    28621 aagtgttcct tcgctgactt cggccatcac ccaaacctcg cctgagtaat ggggctgggt
    28681 tttggtcaat ccggcaatca tatcctcgag tgctttcttg tggaaatcga ctctattgtc
    28741 cgacaatatt ttttcctgtg cggcttcatc aatttgggta ggatctgtaa tatttaattc
    28801 tttcagtttt gccgcaagaa tttgatactc ttccgtttct ttttcggttg cttcgtaaga
    28861 acgatcaaac aaattttccc gtttggctgg cagcagataa ctgggtttgc tgtcgctgga
    28921 agttttgttg ttctggcggt ctttggatgc attttctatg ataatgcgag ccaattcctg
    28981 cgcagtttgg acttcttgac attttcgggt tgtcttgccg ggaggaaaaa cacgaataac
    29041 acgggtttta gagccgctta ctccaattgc tttggcttta acgtcattag cattccactc
    29101 tatcagctga gcagcaatgg cttttcgtgt gcgttcgaac gatgcaaaaa ggggatattc
    29161 atactttgca actgttatca tagccggaac agtgcgggga cgcactactt gactgccgcc
    29221 ggaaacaatg cgtgtaaact ggaattggcc gttgtggtat tctgtgcccg taacataagc
    29281 gatacagggt atccctaatt cttctgccat ttgcgcggga acttgggctg tatcgccgtc
    29341 aacactctgc attcccgcca cgatataata atccttgctg gaagccagca ggtcggtaca
    29401 gattttacgc acggcatagg ccagcggatt ggctgttgcc caagtatcgg caccgcctaa
    29461 agcccggtca gtcagcagga ctgccatatc ggcacagcga cttagggcat aacgcaaaac
    29521 atcggcagcc atcggcggcc ccatggtcag agcgatgatt tttccaagtg tgtcgccgga
    29581 cagatgcttc atatgctgtg caaaggcgag ggcttgagca tccagttcat taataacgct
    29641 gggcagacgg gttcggatga gattgccggt tttagggtca aaggcgttat ctgtaatctg
    29701 ggagacatcc ggcacctgct tgaccaatac gatataatgg ctcatgaata actccgattt
    29761 atatttgctt ttccggtttc tttctgattt ccgaaaaaag gaaactataa ataaaattct
    29821 gctaaggtct atcaaaatcg gccattggac tatttcaaac accttgattt ctaagtttga
    29881 gtgtaataac catacggcat ccgagcaaca gaccgcagct aaaaacagct aaaaccggca
    29941 aaaaaatgtc ataatttggt cattttgttg tagttttttg gctattcctg ctgaaatcga
    30001 ttgtaccagc ttgatttttg gtgtttttaa tgctaccaaa aatatgtgga aataacgcgg
    30061 gcacagtatt tgaacaaaaa tatgatttaa gaggtagaca atggcagatt taaaaaagtt
    30121 gagtgaagcg attatacgcg gggatcagcg tactgctgtg gaagtaacca aagaggcatt
    30181 gaacgaaggg gtggctccgg gaatgatttt aagcgaagga cttattgccg gaatgaatgt
    30241 aataggcatc cgattcaaag ccaacgaagt ttatagcccg gaagtgctga ttgccgctcg
    30301 cgcaatgaaa atggcaatgg aagttcttga accggaacta gtcaaggctg gggtcaaacc
    30361 cttaggaaaa gcgatgatag ggacggtaca gggagacctg catgatatcg gaaaaaatct
    30421 tgtatgtatg atgctaaagg gtgccggttt tgaagtgatt gatttagggg tagatgtgtc
    30481 tccggacaag ttcgtggaaa aagccaaaga aaatgcaccg cgaattgttg gtttaagtgc
    30541 tttgctgacg actacaatgc cggcaatgga aaaaacaatt cgcgctatta aagatgcggg
    30601 attgtctatt aaaacgatga ttgggggtgc tcccgtaaca caggtctatg cggacaaaat
    30661 aggcgccgat ggctatgcgg ctgatgcagc aactgctgtg gatatagcca agcgtcttat
    30721 gaattgagca atcctaaaaa cggatggatt acaagaaaac cggtgttttc aacaccggtt
    30781 ttttaatcga ctggatcaat gaaatgatgg gggacaaaaa aaaagagcat tcacaatgct
    30841 ctttgaagat acaaaaaaag aaagaggaga ggtgatatct gcttatggat tgcctgtcgt
    30901 cggtgcaggg tgtttgtttt cctctttttc tttctggatt gctaaataaa tctctttgcg
    30961 atgtacggta atattgcggg gggcgttgat tcccagacgc accttatcgc cgcggacatc
    31021 tacaattgtg atttccacgt catcgccgat cataatcgac tcatcttttt gtctgcttaa
    31081 aaccaacata aatgaactcc cgaatttccc tttctcagcc ggaccgactt aaatccgtca
    31141 gacagctcga aacctctgtg ttccgcatta cttatacgtt ttacaacaaa gcatgagcat
    31201 aaaaaagttc gtaaactcct gcaccgtata ggcacaagta atttcgactg ttttaagcag
    31261 aggaagtaat gaaatttaaa aaaaatgcaa atatttgtca ggcggttttt acaaattctt
    31321 ctaacttcat tatatataat gaattgtaaa aatcaacagc ttttttgaac agaattaatc
    31381 tgtcggcagt gtagatttat cggacgtgtg agaaatggaa gagttgtgct taaattgaat
    31441 ggatatccaa tatcctgtca tagcccccaa gacagaaaag ggggcatact gtatcggaag
    31501 aatcgcatat attgtatggg tgtaaaaatc aattggccaa gttttgacca gatgctgcag
    31561 gtcttgtgag tcaataattc gcgtaaatgc accgatgtag agcaaagatg cgcctaaaat
    31621 gacggggata taatccgttc caagaaagta ccgtgcaata aatgccgcaa gccccacggc
    31681 tacaaacact ccgaatgctg cctgacgatg gccgggctgt cctgctacag agccgagtac
    31741 ttcgtcaaac tgattgatat cttgggcaaa gatgccgatg gtcagatagg ttataacggc
    31801 cccagccgca acagcaagaa accagtttct ccagttaatc gtcgtattaa atgagaaagg
    31861 attcctgaat tttacagcca gcatctttag ggcaagcagc ataccaagat agcctgcagc
    31921 acaaatacca agccaaaaca gcaattccca ccgcataaag gcatacattg cattccgctg
    31981 ctgcagcgca gagttagtca gcagaatctg ccgcattgca ccgctgttga gtgccagaac
    32041 cgcgatgccg gccggtgcgg catagggagc caaattgccc ccgagagggt aacaaacaaa
    32101 cgctgcggct gcgcctgctg tcaagccgag accgaccaga atcaggcact gcacaggcgt
    32161 catttgcata gagaagagac tgacagcacc aagcggatcc tcaggtcgaa ccaatggcca
    32221 gcccaaagct ccgacaatca atgccccaat gctcagtgaa gccaaaatgc ggagtcgcag
    32281 cagccaatga atattaaata aacccagccc aaaataaatc aatgctattg ctgcaatcac
    32341 acgcaatgcg attggataca gccattccat agttaaaatc ctttcatttg tgcggccaag
    32401 ataccagagc cggccggacg ggtcaagata aaacgattgt catatcttaa tctttaccca
    32461 gtggcgcagc ttaaaatacg aagcagtcgt aaataattgt ttacacccct ataagcgggt
    32521 gcggcaatgc tccttaaatt gctgcaacta tgttcttttc cgtgattttg agtccatgag
    32581 gatatattgt attgccatta cgaggcagca gtatagttaa atctttgatt gagtacaaat
    32641 gtgaggattt attaaacccg cataattgaa gaggtaaagc gttctgaata ccacacaaag
    32701 aagattttat acatcattat tctttttgct gcaaatggtt gaaaaaaata aggacttgaa
    32761 attgaaaact tgagaacaag taactatcga ttttttattt aacaactccg attttttggg
    32821 gataatattt cttgccctta cgttcaattt taccttcgtt taataggaga ccaattattg
    32881 accaagatag aaaatcttta ttgccccccg aataatccga gtgaatctca agtagatcag
    32941 cgatatcaac atttctgatt ccattagggt gagattcaat gatttctaca atcgcctcct
    33001 tcaataaaga caatcctttt tgtgcttttt cctgaggtgt cataaaaaaa tatcctttga
    33061 atatgtttta gcatacattc aagagttgcc acatttttta gtaccagttt tcatgaaagt
    33121 ataaccgatc tgttctgtct gaatattgtc ttatggaaca agtagggagc aaaaccaatg
    33181 cagatgctgt gagggtgcaa agccccgcaa tggatggtgt taaaacatga tacagccccg
    33241 cgtacgcctc cgacgtctgg taatccaaac tcctaagcag cctatagtga ttcttcatat
    33301 tcttgcctat tgccgtataa gaacaaagaa aaaagaggaa cagaaaggtt tagaattgct
    33361 ttatacacag gcggcggtgg gattcgaacc cacgaaataa cggttttgca aaccgtcgcc
    33421 ttgggccgct tggctacgcc gccgaagatt taatacctac catacagcaa taccattgca
    33481 atttcaagaa aaattctttc gctcattcgc cacgacaaat cagatacaga atgcctcaat
    33541 aggctgcggg aataactcat tcaccagtcc ttttgaatgt ctaaagagca gttaaaacaa
    33601 taaaatattc cggttgacca gttaatcttt attggcagga atataacagc agcccgttcc
    33661 agccctgaat ctgcacaaaa acgatattaa aaggcctaaa atttggcagt tgccgccgca
    33721 tccagtatgg catcaaaccg catctgggct ttgattcagg aacaaacaat aaatcgtatt
    33781 aagtagtcag gcactaaact atttgcataa gactatctta ggatttataa ttccgcttcc
    33841 tgctgcagcc atacggcagg gcttaagaac atactgacat aatacaaaag ttaacaggag
    33901 atttttgtga gcaaggcgaa agttactatc gtaggtgctg gcaatgtagg ggctacagct
    33961 gcttttattg cggcaagcag ggcgttaggg gatgtggttc ttctggatat tgttcccgga
    34021 ctggctgaag gcaaagcgtt ggatatcgca cagtccaaag ggatttttgc atccgcaggc
    34081 aaagtcacgg ggggaacaga ttgggatttg gcagcggaca gcgatattgt tattataacc
    34141 agcggtctgg ctcgcaaacc gggaatgagc agagatgacc tgctggccaa aaacgccgga
    34201 attgtgaaat ccgttgccca gcagatcgtt cgcacctgtc ccaacagttt tattattgta
    34261 gtatgcaatc cgctggatgt gatgacgtat gttgcagcca aagtcagcgg ctttaagaaa
    34321 agcaaagtaa ttggtatggc aggggcattg gattcggctc gttttgcctg ttttgcagcc
    34381 gacgaactga aatgcgacat tggtgatata cagggggtat taatgggggg gcatggggat
    34441 gatatggttc cgctgccccg atttacatcg gtggccggca ttccgctgcc ttgtctgctg
    34501 ccccaagata aaattgatga attgatcagc cgaacccgca acgggggcat tgaaattgtc
    34561 aatctgctgg gattcagtgc ctattacgca cctgcagccg gtgccatccg aatggccgaa
    34621 gctatcctga atgactccaa gaccattctt tcctgctgtg ctttctgcga cggtcaatat
    34681 ggcattcata acacttatgt gggtgtcccg gtaaagcttg gggccggcgg tgtagaagaa
    34741 attattcttc tggacctgaa tgagcaggaa aaagcccagc tggctgcatc agctgcgcat
    34801 gtcaacgcga tggttgaaaa ggttgatacg atgatttgac cttattccga ctggaaagag
    34861 ggaattgctt ttccttcttc cagtttttta ttgaacagcc ggatttggag gtcaatggct
    34921 cccattgccc agtcgatata atggagttta tgatagggtt tattgtgccc ttcgtgaagc
    34981 aatccatcat aaatggcatc cagaaaacgg tgttttgtac tgtgcgaaat aggagtgggc
    35041 tgcaatgccc attctttaag ttcttcataa tcatttcgca gccgctggct ggaggccagc
    35101 tgggcggcag tcagataatc gcccggattc agcgccaact gaatcccttt gagagccata
    35161 atcagaggtt cgtagctggc acgccgttcc aatcgtataa atttgtactg gtgtatataa
    35221 atagcctgtc tgagcggccg aacttcttgc gggtcgcccg ataactgcca gcggcgcttg
    35281 ctggttcggg caaacataag ctcttcgccg tagtcgtccc gttcaatttc ttcgtttctc
    35341 agtcgtgaaa tccatatctg gcctgtatct gtaatctgtg tgccgccccc taagacaaac
    35401 aggcacttga caaaactcaa aggcagtgtg gattgcttgc cgatctgatt tcgacgaagc
    35461 tgaaaagaca aatgcagact gtgcggatta acagggaaaa attccgttat ttcacgaatc
    35521 agccgattga gaacccacgg atctgccgtt gcgggcttgg aaatttcccc taatgtattc
    35581 aatcgtcccg gcgcaagttc caaaaagccc cgattcgagc ggttcggctc gccggaatgg
    35641 tcatcgacat agctcccaag cttccatccg agaacatcca tcgcaaaatc acaaaaataa
    35701 cgaaaaccgt cgaaaaccgg atcctgattg gcaatatttt tatgaatggc acggaatccc
    35761 agattgctta attctacttc tgctcccatc ggaaccagtc ccggctggag gttgtttcgg
    35821 atgatggcca tgtctccctg taccacccga gggtcaaacg cattttcagc cgccgctaca
    35881 gcatatttga cataatcgat caaatcgtat gaattcccgg gcggcatcgt ttccagcccg
    35941 cggcgaagaa tcatatcaat tgcatgactg ttggagttaa tcatccgcag tgcaattaaa
    36001 agactcaaga tgcgggtata atagcgtttg gatggacata atcggtccag ctcgaatcga
    36061 aaccggtcat aaatcatcga ctcaaactgg ttcattacgg ccagcttgat gaccagcgcc
    36121 agatagtttt caatatacgg ccgtgcttcc ggagtgttga tcagatgccg catcgaaggg
    36181 catggccgat tcaattcgtg ttccagataa aaatcgctga cggcatcctg acgtaagtgt
    36241 gacaccacca gatcccgctc gatagttccc agaagctgcc ggcgcgcacg gcgttctata
    36301 tctgcatcta ttgagtgctc tcccggacgg gtatgcgact gaaagaattt ttctattcgc
    36361 ttgcgcagcc acagttcgtg aatcaaattt acatgaaagc ggtccccaaa ccgttttctc
    36421 atcgattttg tgctggccag ataggcctga gcctccactg ttccgttggc ggtatgaacc
    36481 tgaactgtct cacggtcata aaacttgcct tcatactgat caataacctt catcgccgag
    36541 gctggaacat cgtagataac aaatccctgc agcttggagg tggtatcagg aattgcataa
    36601 aagtatatat tatcaggact tacgcggcga taatgcggca gcatcgcaag ttctgcattc
    36661 aatacattgc cggttctggc ttctgatggc ttcaatgaaa aactgtatcc gcagacagat
    36721 tcaaagatcg aaggatcgcg aagcgagcca taaacaaata aattcattgg tttttcggtt
    36781 gatgaatctt tcatggtcaa tatattgtat ggatctgctg cctgcctcgt caatgaacga
    36841 ttcgattgac atgagcatga ataagggtca aaatttcata acagatggta tcggccaagt
    36901 ctgctaaggc atacgccgaa cagactgaat cgtgagagtt gtccagtaca gtcaccattt
    36961 gtcccaattg aatatttgga acatcggtaa catccagaat aacctgatcc atacacactc
    37021 ggccgataac gggtaccgcc tgatgggcga ttttcataaa tgctctgttc gaaaaacacc
    37081 gccggtatcc atcggcataa cccagaggga taatagcagc cgttgtatca cgtttggtta
    37141 catacgagcg gccgtagctg atggaatgac ccgccggaat gtgtgcaata tgggtaatcg
    37201 gcgcctgaag tgtaagaacc ggctgtaatg gtatcggagg atttttcatc cgccgggagt
    37261 aatagccata catagcaatg ccgcagcgta ccatatcata atgggcttgc ggaagcttca
    37321 ttacagcggc actgttggca gcatgtatca ggatgtttct gttcctcaac tggtattgat
    37381 tgagaaagcg ttcgaaaact tcgatttgtt cgtatgcata agataaatca tcctcatcgg
    37441 cggtagcaaa atgagtaaaa acgccggcca gttttaagtt gggacatgcc tcaacctgtt
    37501 cgagcatccg gccggcctgt tccggtgcga tgccgccgcg tcccattcct gtttctatgt
    37561 tgatatgaac tttttggacc aacgatgttt ttttaagagc ttctttaata taattcaggg
    37621 aatccagcga agacaaaacc ccgtcaaatc cgctgcgggc gcatacctgt atttcttcga
    37681 tggactgaga aggattcatc ggctccagaa tcaatataga tttatcaaaa accatcgagg
    37741 caatatgcag tgcttcataa aatgtggcta ccgcaaaagc atcggctccg tcccgtacaa
    37801 gaatattcac aatttcggcc agtccgtgac cgtatgcatt ggctttaact acagcgcaga
    37861 atttggtttt tggagaacat aaactccgca gatgacgaac attgttcagc aggtcctgcg
    37921 aaaaaatatg cgcctgcaga tccgggcgga taaattgctg ggttcgatag actctcatcg
    37981 ctttcctgtc tgtaaaatcg ttttacgcaa cttaaagagt tctcggaaag catgaagaat
    38041 tactttaaaa ttagccccgg ttgaacggcc tcctatgcgc ggataatgat gaacaccgac
    38101 ctgtgttatc ctgaatccca gtcggctggc acgtgccaga atttctgtat cgattaaggc
    38161 acccgtgctg atcatttcaa tttggtcaaa aatccgccgc ttatacagtt tgaaggcgca
    38221 gtcaatatct ttcaggttaa gattaaaaag aaaattaatc agggctgtcc agcagaaggc
    38281 gttcagttga cgcttccagc cttcctgccg tttaaggcga taggcgctga caatatcata
    38341 ttgctccatc agcggcaaaa ggcgatgcaa ttcggtgaca tcgaattgtc cgtcgccgtc
    38401 tgtataaaag acatactctt tggcggcggc ccggaagccg ctttgcagtg ccgcaccgta
    38461 accaaggttt accgggtgat gaattacccg aactgccgga tattcccgtg ccagctggtc
    38521 ggccaattgg ccggtaccgt ctttgcttcc gtcatcgaca ataataattt cataatccgg
    38581 gcatatttcc tttaaaacag cgagtgtttt tactgtcaga ggtccaatgg cctcacgctc
    38641 gttgaagcac ggaaagaaaa ccgtcagtga ggtgacgggg tgcgtttttt tgatttctaa
    38701 ggtatctccc atagactacc agatgcggct ggtattaatc agtttggata attctcgaaa
    38761 ctgttctgca aactgacgat agcggtcttt ttcctgcccc ttaatctgca tcagacaacg
    38821 ggcagctatt tctgcactgc caagccggag tagaaatcca ttttcttttt tatacatctc
    38881 ttgattttga gtaaaatcat ccagatatgt ctgcaaatcc gatgttgctt gctccctaaa
    38941 ctgagccagt ccagtcagcc gatttaacgc cagcgccgca gcgggataag gatattcgtc
    39001 aaaagcacct ttggttaatt catacaaatc cccaaaacgc ggagcgtatt gattttctaa
    39061 aatagcgaca gaagttgtca gcttttgaga aaaactgtct ggaaatccgg ccttattatt
    39121 caaaatattg tcgatgaggt ttctgccgtc aggattttcg atatcaacca aaagatgctg
    39181 ggtattatcg agataccctg tcttccaatt cggtgtcaat ttgagggcct gcataaaagt
    39241 cgagtcttcc tgtgattgcg gcatcaaaac tacccacact tgataagatt tgagctgctt
    39301 gtcgatccat agaccgactt ctcgtagctg ttcggcggtt aattttcggc cttccgcaag
    39361 ggcctgctga gcaattggac cgcccccatg aataagctgc cataagcgga atttatcgtg
    39421 attataagca gcttgagcac gtccatccat aaagagtttc agcggaattt ggccggtatc
    39481 cttgtccggc tgctgaccga atgcaatagc gccgccttct gtccaataat taaaaacgcg
    39541 gccccgtaag ttattgatgt tgatgaactg gcatacctcg aagggcttca aatgggaagc
    39601 agtcattcgc ataaaaatac tgtgataccg gttatctgtc ggccaaggat cgagataaac
    39661 ccgcttgaat tttatcgccc aaaaaagtcc taaaagagcc aagaaaatgc ttaatacgat
    39721 ataaccggcg gaaacaatac gaaaattcga agaatctgat gctgaaaaga tttttagatt
    39781 aaatttggct gtaatcattt gccatgcttg ccgcgccagc agaaacactg caggtgctgc
    39841 ggcagaacct gcaagagcaa taaaacgtct ggattgtaca gccatataaa gggtcagcag
    39901 cgatagaaca ataatagcca agtcgatgcg cggccggcca gacggcagat tgcattcctg
    39961 ccgaagcgat ttgcggctgc ccttaatcgt cagttgcggt tttaaaaagt agccgctcag
    40021 ccatgccaaa aggacaagcc ccgtcaaaat gcacagtacg gcaaatgcct cttcttcgcc
    40081 taccggattg ggtgttgttg aggttttatc catccagtcg aaggcaggtt tccactcatt
    40141 gacctgacgc catgattcag catgtttgct gacgctgatt tcaaaagtat gggtcaggtt
    40201 cgtaagatgg aagggattta gtataaccat tgcaataaac gaacagactg ctgcgccgat
    40261 ggtatgaatc aatccacgaa ccggcaatat aacaaaccgc tcttttttga ctgccatcgc
    40321 gaataccaga agagttcctg caatgaagac aaaacagaac gatagctgag aagaagacac
    40381 aaaagatgaa taaattgtct taaactgagg cgtcaggtta acaggaatcc gggcaaaaaa
    40441 ggaagtgcat aagcttagaa accagataaa gccgacgcca atgtggtaag cataaaaagg
    40501 agccggcttg actgttttta tggcagccag aattacagaa cccgctgcaa ggatccccca
    40561 cattgccagc attttgctga taaagggagc cgccttataa tcagaattca ggagtttctg
    40621 tgtctggaca taataatcat tggtcagaaa tttataaatc attaaataga gcaccagcca
    40681 taggcccatg cagcccagac tgacagtcca ccgacgcggc agattcagaa gaaaatgaat
    40741 cccaataaac ggcacgaaca taataaaagc ataaatatag cccccgtgaa cattggccca
    40801 gaaaacaatc agcggaatca tcagccatat cagccgccaa ttgcggtatg ttgccagcgc
    40861 cagcaccaga atatagacgg gcacaagcag attggagtag ccggcaggac gaatatcgaa
    40921 aaatgttcgg ccgaccacca ttcccatgca cgctgctgcg gccgatagaa aattgcctgc
    40981 tccgagcagt ttaccgatgc cgtacacact gaaaacagtc agaaaataca gcgcaaactt
    41041 ccagataacc agaacattat agttgggctt atcttcgctt ccaaaccaga cagccagttt
    41101 gtaataaata agatgcgtca gccagttctg gttaatccag ccggttggat gccaccagcg
    41161 tataaaactg cggctccaag caggaaattt ctggagctgt tcatcagacg ggccagcagg
    41221 gtgagagttg aagctaaacg gttcaatggt atcaacccca tgatgagcaa aatgccgccc
    41281 gcaggccatc gctacccacg tatcgccggc agcaaccata tgcgtagacc cgtaaaatgc
    41341 gaacagcatc atgcccgcaa ataaaaccag ctgcaaccag ccagtccgac gggaggtgtt
    41401 tcctaattca ttcatttatt cctgaagcca cctttggatg cggtccatcc ctttttctat
    41461 ttgttccagc gaacaggcaa aacttaaccg gatattattg ggactgccga acggagcacc
    41521 cggaactact cctacatggg cttgttcgag cagagcagcc gcaaaatcca tactgtcgcg
    41581 tataacagct cctccgatgg tgcggccgta atgagccgag acatccggga agcagtaaaa
    41641 agcaccttct ggctggtggc agcacactcc ttttattgca ttgagccgct gagccatcaa
    41701 gcgtcctctt ttttcaaatt caatccgcat tttttcaatt gtgtcgccct ggtctttcag
    41761 ggccgcaatc gctccaaatt gagcaaaggt aaccggatta gatgtcgaat gatcctgcaa
    41821 gcgggccatc gccttgatga cattcagggg tccggccgta taaccaagcc gccagccggt
    41881 catcgagtag gccttgctga ggccgttgat tgtaagggtc cgctgatagg cgtcggcgct
    41941 cagtgaagcg aaactgacaa accgggtttt gccgtaaaca agtttttcat agatttcatc
    42001 tgacagaaca atcacctctg ttccctcaag taccttcgcc agcgccataa gttcatcggg
    42061 actgtagcag aagccgccgg gattattcgg tgaatttaga atcagcatcg ctgttttagg
    42121 cgtgatggcc tttcgaagct gttcaggggt aattttataa ttagtggcgc ccgttgtttc
    42181 aagtataacg gccttggctc cggccagttt gatggcctct ggataggtta cccaataagg
    42241 agcgggcatc agcacttcat cgccgggatc caaaaccgcc tgcattgccc catagactga
    42301 atgcttggct cccaaattaa ccacaacctg ctcccagctg tagtccagat tgttttcccg
    42361 cttcagtttt tcggcgatag ctttccgcag gtcgggcata ccagccgcgg gtgtatattt
    42421 ggtttgtcct tttttgaggg cattgattgc cgcttctttg atgaattcag gcgtatcaaa
    42481 atccggttct ccggctgcaa atcccaatac atcaattccc tgtgctttca tttccttggc
    42541 gcggctggta actgccagcg tagccgatgg cggtacagat tgagcgcgtt ggctgatttt
    42601 cataaaaaat cctctcatta gtagattctg cttcgtccaa aaattcagcc ggcattaaaa
    42661 cagcttgctg gttaaaaatc aaggaaaata cgcaaataac ccttgcagat tcggcatttt
    42721 aagatttcag gcagataatc cgatggaccg ttatttaacc ggacatagca agtcatttat
    42781 tctcctcaaa tctgtcacac ataatgtttg acacaataaa tgctttttgc gacaattttg
    42841 cccaaatgag caccagaggc ccaattattt gggggttgtc tattttggta tgaatcatct
    42901 ttgctcaaaa gtagtttaat gaggagatga agaatgtatt ctaaaaatac aaggcaccca
    42961 gttgcggcag taattgcagc gattgtcttt ttttcacttc aggcagcggc gcaggaatca
    43021 gaaaagactg tttcgtctcc aaaggagctt tatctgccga aaaaagcaga ccgtgtgccg
    43081 gaaaacaatg actacaacag ccaagatagc gaatactgct tcaaacgcat ggttcaatcg
    43141 gataatattg ctattttttg gagcaaagaa tatggcgatg atcccctcgg caatccggat
    43201 gcaaggaagc gctttgatgt ccagtttatg ctctctgaat gcgaacgctt ttataaatac
    43261 tatattgatg aactgaaatt ggtccagaaa ggcaattcta tcagtgataa atacaagatg
    43321 cttgtttatg tttttggcgg cgaagggggc acagcatttg gcggcggagt ggatgagaaa
    43381 atcggcgtat tatggacacc tgccgtgcgg gtcaataagc ctccgtatgg tgttttagcc
    43441 cacgagatgg tacacagctt tcaatacata attggaatcg acacgggtaa acacctgcgg
    43501 ggatccattg tagagatgtc agcacaattg atgctctggc aggtctatcc cgaatggatg
    43561 acatttgaaa actaccatct tgtcgatttt atgaagaaaa cccattatgc ctttctgcat
    43621 cctaccaata tgtaccactc tccctatgta ttggaatatt ggctcaacaa acacggcaaa
    43681 gagtttttcg gccggctgta tcgggaaact ctgccgaatg aagaccctgt tacaacttat
    43741 aaaagaatac tttcggttac acaggatgag tttaataatg aaatgtttga tgcatcgcgc
    43801 cgatttataa catgggatat gaagcggatt gaaaaagtcg ccagacccta cgccaaccag
    43861 caccaatgca gtcttatacc tgccgaagac gggtggtatc gaattgcccc ttcacggtgt
    43921 cctcaaaatt acggctacaa tggtattagg ctcaaagtac cgtcgccggg aacagaagtt
    43981 gtcttacatt ttaatggcat tgcaggggcc gagggctata atgccgtaaa aatagaaatg
    44041 gccggctggc ggtatggttt tctggcttcg ctcaaggacg gcggccgcat atacagcagc
    44101 gttggtaagg agccacaagg gatattggca tttacagtgc cggagaatac ggaatacttg
    44161 tggctggttg tgatgggggc cccggtcgaa cactggcctg tggcaggggg cagaggacgc
    44221 agagccgata ataccgccga agagcaatgg ccttatcaga ttcacctgaa agggacagca
    44281 cctgatgatt cagttattca ataattgatt gtaggttggg aattgtatgg ggtttcagtt
    44341 ccacaggctg ttctcggtag tctgaaaaaa acctttatta ggcttatttt gcaaccgcat
    44401 tattgcaggc caacggattt tcggttataa acactgcaat agaagacaga ttgcaaaact
    44461 gtttcaattt cactcaacaa gaaaatagcg cccgtagctc agttggacag agcaacggat
    44521 ttctaatccg tcggtcaggg gttcgaatcc cttcgggcgc ggtcagaagc aggccagtcc
    44581 aaataggcgt tttaagcaat tttttacttg tttttttcaa aaagagtgat attgtatttg
    44641 cctgtttttg tgaaacagag aacaaatctt tatttataca gtaacaggat tatgcaaaaa
    44701 tggccatttt agtgcagaaa ttcggtggga catctgtcgc caatgctgac aaaatccgcc
    44761 gtgccgcagc ccgagctctg gcagcccatc agcggggaga gcaggtaatt gtggttgcct
    44821 ctgcccgcgg aaaacagacg gacgaactga ttgcagatgc gctggaagtg aatcccaatc
    44881 ccccgcgcag ggaaatggat atgctgctga gcacaggcga acagcagaca gtatccctgt
    44941 ttgctatggc tctggccgct atgggccaaa aggcaatcag ttttaccggc cagcagattc
    45001 agatgattac cgataattcc cataccaaag ctcgtattcg cagcattggt accgaccgtc
    45061 ttcgcaaaaa gctcaacgaa gggtatattt gtgtggtagc cggctttcaa ggtgtggatg
    45121 aagatgggaa tatcacaaca ctgggccgcg ggggctcgga taccagtgcg gtcgctttgg
    45181 cggcggcgat gggggccgac tactgtgaaa tctataccga tgtggacggc atttattcag
    45241 ccgatccgcg gaaatacccc aaggccgtca agctggagca aatcagttat gatgaaatac
    45301 ttgaactggc cagtctcggg gctggtgtta tgcacagccg gtcaattgaa ttcggcaaaa
    45361 aatacaacgt aaaaattcat gttcgaagca gtatgaacga taatcccgga acccttatta
    45421 cacatgaggt acctcaaatg gaaggcattc tggtctcagg cgcaacgtca cagaaagacc
    45481 ttgcaaaaat cggtttaatc ggtgtggaca acaaacccgg caatgcggca cgcatttttg
    45541 cccatctggc aaaacacaac gtcagcgtca atgatattat tcagacggaa atgagtccgc
    45601 aaaaagccca tctgtcattt acagtagcta cttctgattt aagtgaggcc aagcaggcca
    45661 tcgaagaaat acgggatgaa gtgcattgtc aggaggtgct gattcgggaa gatatcgctg
    45721 aagtttctgt tgtcggtgtt ggaatgcgct ctcactgcgg tgttgcggaa aaaatgttta
    45781 aggcgctggc ggataaaaaa atcaacattg atgctattac caccagcgaa attcgtataa
    45841 gctgtctggt taaccacaac gatgtcgata cggctttagt ggccgtatgt gacgctttcg
    45901 aattagacaa gcccgttaac aggcggcgtc acccctgaga aattattgcg ctgaaatatt
    45961 aaaccggcag caaggggcat aagccagact tgttcggttc ggcgagggag gggatattat
    46021 gttttttctc tatggccaag aaaaagagaa aaccccgata tgcaatgcgc cgcataacaa
    46081 gaaatgtaat tgccgcggca ttcttgctta ttgtagtctc gtctatcacc ttgttggacc
    46141 gtcaattcgg cggcgcaatc cggaaaacca ttcggcaggc tgcatacacc cagcctgatt
    46201 ggcaaaagta ccatggcaga cagtttacag tagtggaagt cattgacggc gatacactgg
    46261 atatcgatat accggacaaa gaatttacaa atacccgtat ccggctgctg ggtgttgaca
    46321 cgccggaaac caaacacccc accattggtg ttatgtatta tggtcccgaa gcatataatt
    46381 tttcaaaaca gacggccctc ggccaaaggg taactatctt gctggatact gtcggtgacc
    46441 agcgtgaccg atacggccgt ctgctggcct atgttgtctt gcctgacggc cgagtactca
    46501 atgaggaaat tgtccgcctg ggctttggct atgcccattt gggttttcct cacaccaaat
    46561 atgctgcata cgaagcacta atggaacaag caatgcaatc taaaaccggc ctctgggccg
    46621 gtgttgcccg cgacgacttg cccaaatggc tccgtcaaaa acgtcccgaa ctcctccgca
    46681 attctcgctg aggcttgaat gtctcgtaaa ccctgcggcc ataaggaagg cactaaaaag
    46741 ccccggacaa cttcaattaa aaagccccgt catgtatgac ggggcttttt gttggtgtta
    46801 cctgcaataa gctaaacgtt aaatgcttat ttcctgcgac gcaacagcag tccgcccaga
    46861 cccagcagaa ccatcgttgc cggttccgga atgacagtaa cggtcacttc atcgaactgt
    46921 actatcctgt cccccgtact cttgaaaaga atgccgagtg ttcctccgac cgatgcagga
    46981 gtggctgcaa tcccaacagc atcagtgtac cagaccgcat tcggcatcca ggaattcatg
    47041 ttctgtacgt agctgccgat gacattggcg ggattgtcat agaacaaagt ggccgtccat
    47101 tgacctaaag tcccagtcca accccactcg ccgccgatat agctgatgaa aaatacatcg
    47161 cccgcctgaa tgacgtacgt gctcatattg taggctgcat cgccggccga cataaagcct
    47221 gcattctgat aaacattagt ataagcttca gttagccacc aagcacccgg tccctcaacc
    47281 cctgcgtcat tcaatgagcc ggcaggatag ttcgaccacc caatgatctc agtagcagca
    47341 ggagcgtcaa aacctttaaa cggataagta gttacagccc cggaatcaaa atccaggtta
    47401 gtgaatagcg acgagatgtc tgccgcctct accgctgtgg ccaacgcagc caccatgaat
    47461 agaacgaata cgcttttcat ctttcaatcc tccacaaaat aaacatattg gttacagtaa
    47521 aatgttttac ttaacggaca atacttcaca cactatcatt ttaaacaaat ttgagagatg
    47581 ttgaaatgga ttctgacatg tttttctctt cttccacacc tcttccaaat taactatctt
    47641 tccaactacg aagatatata aatacatata acgaattcat agctgctttg tcaagaaaaa
    47701 tttatagtat tattggaaat aggaaaaaca gagtgaaaat agggacagtt gcctgttact
    47761 tacgacatcg caaagaatgc tttagtttgc agataacgtt tttttttata aggacttaac
    47821 ttctaaattt gaagatcaaa cttcaaaatg ctgcaaggca tggcttgcac cataatatcg
    47881 aattgccaaa actcgaatgt tcaaaaacaa acgccgaatg gccgtgtggc agcattcgtc
    47941 cccgcggaac atcccgaaag caagcggaca ttctcaattt gattaatcct gttgtcctgc
    48001 ctgccaatcg cagctcgcaa agtgcagacg ggtcccaaaa atcagtgaaa aaatccgtga
    48061 ttccccttag ggctctctga ctgttttaaa aaaagcagtg ttctctgtac ctttgttgct
    48121 gaattgaaaa tggtaaattg aaaattctta gtttggctat cattaaacat atggtacaat
    48181 tcgcataaat ggataatacg atattgaaaa ctcaaaatcg ataaaagaag gcagacatga
    48241 cggagaagga tgttaaaaat ttgaaatatc gcggcaaaaa tgcggtttga aaaaacgaac
    48301 cgattttttg gataaacagg acgtctataa gtattttata ttgagtggct tatggctatt
    48361 tcgggagccc gtgtatcctg taggagaatc caattcaacc caaataatat tgtgaaacgc
    48421 aagggcaaaa tccaaaaaca acaaaacgaa ataaagccaa tacttgaaga agcacaattt
    48481 ttctatgtcc ttttttatcg ctgtttgctg catgctgaag gccggcggca atcataaaat
    48541 tgtagaataa aaaaagggag attttatatg acagaacgca aaacagtact agtcccaata
    48601 gctgatggta cagaggaaat cgaggccgtt gtaatgattg atgtcctgag acggtctggg
    48661 gcagaagtaa cagtcgcttc tgttgacagg ttgaatatta tcgcctcacg gaaagtcaaa
    48721 ctcacagcgg attgcttgat tgacgactgc aaaaatcagc aatttgactt aattgcactt
    48781 ccggggggaa tgcccggagc agagcatctg cgtgactgcg ctatcttggc tgatatgctc
    48841 aagcagcaga gagcaaccgg ccggctgtat gcggctattt gtgcatcgcc ggcagttgtc
    48901 ttaaatgccc acggcctttt agaaggcaga aaggcaactt gccatccagc ctttcgagaa
    48961 aaattgcctg accagtcagc cgcatccaga agagtggcag tcgacgcaaa ctgtattacc
    49021 agccaaggac caggtaccgc aatggagttt gctatagcat tggtccgctg tctatttgga
    49081 gacagtcgcg ccgaacaagt tgctgaacct atgtgttttc gcacataagt ttatcttttg
    49141 gctgacaaat tgttctttta ttctaaaggg gttttactaa ataaatgccc gatagaaatt
    49201 gtctggtttt gctccaattg tattagtcaa tgtaccaaga tgctgaatgg tacattccac
    49261 aatatccccc gggtgcaggt agcttccatc cgcagcggca acaccgtgtg gcgtaccggt
    49321 aataataaca tcgcctggtt ccaatgtcat taaatgactg ataaatgata ctatttgggc
    49381 gattgtaaaa atcatctgtg aagtattggc tttttgtcgg gtctggccat tgacttttaa
    49441 ttccatatca agattttgcg ggtcatttat ctcgtctgct gttacaagcc atggtcccaa
    49501 gggcaaaaat ccatctgccc attttcccat catccaatca aaaaacacat cacggggacg
    49561 ctcctttcgt ccttgtttga atgtaacgct tctggcagat atgtcattgg caatactata
    49621 tccggcgata tatgaaaggg cctgttgagg ggtaattttt gaagcattgt cgccgataaa
    49681 tacggccagc tctatttcat aatctatctg ctggctgtaa acaggccaag gtatgactgt
    49741 ttggtggccc gtaatgaccg atgccggcat aagaaacggc caaggattgg tttttaccgc
    49801 ttgatcatca aaggttccat cctgccaggc agtttcctct aaatgctttt tatagttgcc
    49861 ggccagtcct attacttttc ccggatgcgg aataggagcc cggagcttta tatgatccgg
    49921 ttttatccgt tcttgaaccg aatgttgcaa ttcctgcaat atttcaagag ctttttggcc
    49981 tttaataagt atttccttaa cactatgcag ccgtttggcc ccttgtgtgt gagatgggat
    50041 gtctataaat ccctcgtttg tcagaatacc acacgaaaca gcatgatgat aatcatatgt
    50101 tgctaatttc attgaattcc ctttcaaaaa acaatgtctc ttgcttgaaa gaacgtcgcc
    50161 aaacataaat ttcttgcaaa taacagataa atactattaa atagtgtccg tttcttctga
    50221 taattacaaa gaggattagt atgttcaaaa ttttcagttt cagaaaaaag aaagcctgcc
    50281 cccaatcgga tatcttgggc agcgagatgg tttcgcggga agattttaaa gcgtcttata
    50341 acagccttga tgcttcggtt gtgccgctgc gcccagccga gccggttatc cagaagaaaa
    50401 aagattctgc agaggttttc aatgaggctg tcaatcttct ggtagagaaa ctccaaagca
    50461 tcaatgaaaa ccttcagcag caaatccatc aaaatcggca gcttgtagag cggatggatg
    50521 cgctgccggc tgtttt
//
LOCUS       DAOTBO010000012        50495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_565_length_50495_cov_5.809675, whole genome shotgun sequence.
ACCESSION   DAOTBO010000012 DAOTBO010000000
VERSION     DAOTBO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 50495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50495
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_565_length_50495_cov_5.809675"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLE88_02655"
     CDS             complement(<1..696)
                     /locus_tag="PLE88_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOM75397.1"
                     /translation="MKTAEAAVLIIDDNQDAVRQILEVLARRGIRGLVASDLRQASAM
                     LDKSPADLIFANVPIDQPAESFRFIRQIHSAFPELPLVLTGQIQTGTFQPQMLLDMAV
                     QAVPVGCRRFLLKPLERSAIEAVLDTLVPNQAVAAAHWPASGEEGYPIIGKSPRLLQT
                     VELAKKVAATSIPVLISGESGTGKELIASLIHYSSRRAGGPYLQVNCAALSDTLLESE
                     LFGHEKGAFTGAYT"
     gene            complement(693..1670)
                     /locus_tag="PLE88_02660"
     CDS             complement(693..1670)
                     /locus_tag="PLE88_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75398.1"
                     /translation="MMDALRPFSEDRQIRMQHHPKNCCLSAAVLAAVLMTAAASRTGL
                     GQNLAQESDFFQLLENDLQIETKEMPNLTAEPNTVTITEKTAAIDKQAASSDLIHLLP
                     PPRTAALTAQQQVWQARISASPSFSSNPTKEELEGLIRRIRSIEIKPKMPAEPTAAVS
                     VPAPAEKPAEKSAGGEPQQADDAGIVKTAAADGRISAQTLALFRQSAQHPNALKNPYQ
                     LGEILYEGGCLKEAAICYKEALNRLNSQPTSADDKAWLLLRLGDCLRADNPAEALDSY
                     RRVIQDYPNSPWTALAKTKSMWVEWNLQVQPRKLIEQNPLSTAEEIESK"
     gene            complement(1667..4153)
                     /locus_tag="PLE88_02665"
     CDS             complement(1667..4153)
                     /locus_tag="PLE88_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75399.1"
                     /translation="MTDQIHNNNLDALGQEWSQRIKDIRKLAQSESLSDAKDDAGTVS
                     PSDELLSGLPDDTVSDNSAILREEIKHGAAAAGQRQLYPAIGTNQSRRIGILKQLAAG
                     VILSVLVIGIYEILTYLWVCHSIRMRAWGQTDDGQNKVGQTAALQKPAILPDEHPISL
                     KIAQDYYRQGNYAYARAIYSKILDGLEKNEDDGLQEFVKLKTALCFHKEGNSEQSYGL
                     LKNLQVSHLPLIRILANYYLCQAEMRNHQYLSARSRAYQALALLDCVHPPLSGFSRLR
                     KTCDFLAAQAVSRQVLALSDADKELPAALWNWPGLDILSEIDEQSLCAVLNTGAAEFS
                     RALTGPVIEIADRQPVRRWTVISNGTSIGELTSRFAVNTGSNVYWDIASDKTGIHNRP
                     VSLYLRSATEPDCVKIAAGCAGLAAVPDTQTEIRLFNPADYNRVSEQIDLLAKDALSC
                     WQRFLVVYDEDAGHANVHFTMGLLEAVSGRTNQAVAEFLTAAHRFPNSELAPYALVRA
                     AKHKADLYDYAGTYTLLKDTAEQYPDYPNIEQVYLLLADAAVKKGSASEAAKLYSRVY
                     YLNRSPESRLSAALTAGQLFYQHGDFENAELWFNRYITDENARQDRQFHQACLLLGKM
                     LRRQGNSKSAADALLLALSGNLSQADYVEAVTAVVEEYIVQERFIQSLDILNEALRRH
                     LTQQEMVHLLVLKSRVFRGMGLTDKAIAFLNDKESYLLDNSLKAMVGSELSQCYMEQG
                     NWDYAYKKLMQTLEWVQSGPLMHQTMLRLAQVCVNRGLHTQASAVCSQLISLNPADSI
                     RQQAAEIQAGIYQTQKHYDLAVLELLGQNK"
     gene            complement(4474..5904)
                     /locus_tag="PLE88_02670"
     CDS             complement(4474..5904)
                     /locus_tag="PLE88_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM75400.1"
                     /translation="MSINNIVIIGRQREIQTLAQTFAKNVYAADDISEAIELTRRFSP
                     DLILFGTHFTAAEIDGFQQAAHHIANIPVVIVGTGKEGEQNSCGPIKKDNVYYLNSPG
                     DQSSLIRIMELIKHKLPLATYNNGDFFADDIAASAGLAGKSPAVLYALKMIKVVGSSA
                     CNPILITGEMGTGKELAAYAIHLQRSPGRDMIAVNCASLNANLLESELFGHEKGAFTS
                     ADREKIGLLEMAGDGSIFLDEISEMPLDLQAKLLRVIQEKKFRRVGGTREIVCRATII
                     ASSNRDLRQEASNGRFRADLYHRLCIFPIHLSPLRSPSRRSDIRLLAEYFIRTQTICP
                     EKAKWNLSITQLALEALEAYDWPGNVRELRNVIERAVLLETTDKIGLNSIIINPHEPK
                     DFFNSDSSEKIKDYSLAKAEQELIARVLRQTNGRKTKAAELLGISRATLYAKLKQHNI
                     VDAESDEQNNTTINPSDLMPESAAIA"
     gene            6501..6902
                     /locus_tag="PLE88_02675"
     CDS             6501..6902
                     /locus_tag="PLE88_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOM75401.1"
                     /translation="MSEVLSASSLQIDYMKLLTAQLQNQNPLEPLDNADMTAQLAQFS
                     QLSQLEKMNTAFADVLKSVQRQYASSLIGKEVSFVGETGDGSVNLISGQVDQIANIDG
                     KLLLGVGDYTLTLDDILRVKTSVESAADSDR"
     gene            6936..8606
                     /locus_tag="PLE88_02680"
     CDS             6936..8606
                     /locus_tag="PLE88_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOM75402.1"
                     /translation="MSSALSAGVTGLQAHQKMLDIAGNNLANVNTTAFKASRITFSEL
                     LSETVKKASQPTSAIGGTNPQQLGSGVGISGITPNMTQGSIVNTGNPLDLAIEGEGYF
                     VLSSGSQNIYTRAGAFSVDANSNLVDPSTGYVVQRIGSIGESDGFQIPGNSNIKVPYD
                     VAMPASATTLVRLAGNLSSDASLDTPQTNVLKSNIALRTVSGELAASSTLFSQLSSGT
                     FESAAITVSGYAHDGTALTPATISIADDGSTTIGDFLSDLNAVLGADNAVASLQNGRI
                     VITDAQSGYSRSDIKLEFSNSGTADIEMPAYFETAVVGGSEVKNINITVYDSQGGAHV
                     LSGALVRTNTDNTWDMILTSVTGDISSIAMQDRRINGIRFNAADGAFAGLAESSVPQF
                     TITFAHDPSNPQTIEIGLGTEGRLDGLTQYSGNSTAVAREQNGYPAGRLTTVSVNNEG
                     ILIGAFSNGIKKNIAAIQVALFQNPAGLEALGNGYYIPSANSGEAVATQAMNGGAGSI
                     HGGALEKSNTDVASEFVNLIQAQNGYQANARTIKVANDILSELTNLIR"
     gene            8624..9895
                     /locus_tag="PLE88_02685"
     CDS             8624..9895
                     /locus_tag="PLE88_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75403.1"
                     /translation="MTEINSIWFILNEVLIGLTPLERWDALTKYHSSPAASQRLLMIS
                     AAAVGAVIAAAVIWNWLNRQSAAKTDNRRFDDYAGKKQLTDQQKQLLLRVVRKAELKQ
                     KESIFTLPDVFDQTVAGIIEQIRQTKGISEALRLEEQFSSLREKLDFRQTAPDRQMIA
                     AAQDSRQIPVKKKLYIRRNPRMSGELEATVIDNSPAGITIRFPEPAEIVFGQPWTCRY
                     YTGGFVAEFQTVPVQCRGTVVVLRHSDKVRLVNRRRFVRVPVRKAAFVADCSLGRSPF
                     VPRQMSRRKITALDPVSGLSQTDFEPPQFVPATVTELGGLGVRFETSLNVQAGDRIVL
                     VLRLEETEMPMEYQQPPGGEKGITGIARVVRAQRKESGFSIAAEYIYLEDEQLDVLVC
                     AANAVLHQLNRGQHSAAAVLSSRRVPDAAAV"
     gene            9920..10651
                     /locus_tag="PLE88_02690"
     CDS             9920..10651
                     /locus_tag="PLE88_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HOM75404.1"
                     /translation="MSEISAQVGSAIRSLTQEFDLIAHNLANVSTPGFKRRYNDFSKS
                     LSALEQTAGPAENSRKPANIAVDFTQGSFIETGRTLDMALCGKGFFVIETPQGPLYTR
                     NGAFRLNENGQIVDMIGRIVAGESGPITVPQAAGLSQINVAADGTVSAAGVPIGKFKL
                     VDFLDKENRLTPAGLNCFAAPQNMDSEPAVGLVVKQGFQESSNVQLTEELVDMIMVSR
                     LYEANMKLISKSGETAKNLLDVAMS"
     gene            10695..11483
                     /gene="flgG"
                     /locus_tag="PLE88_02695"
     CDS             10695..11483
                     /gene="flgG"
                     /locus_tag="PLE88_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOM75405.1"
                     /translation="MLRAFSTAATGMSAQQMMVDVTANNLANINTTGFKRSQIDFQDL
                     LYSKLRDFGAEVSSGIKTPGGMEIGSGVRAGSTVKVFSGGELVNTSRPLDIAIAGDGF
                     LQVRMPDGSLRYTRDGALQINADGELVTAMGYSIEPSVSIPSNAVSIDIGKDGSVNVL
                     DDSGAQSVVGTLQLARFVNPSGLSSEGDNLLAETEASGTPITGTAGENGFGMIQSGFL
                     EKSNVQMVTELVNLITAQRAYEVNSRAIKAGDEMLKNAIQIAGY"
     gene            11501..12478
                     /gene="flgA"
                     /locus_tag="PLE88_02700"
     CDS             11501..12478
                     /gene="flgA"
                     /locus_tag="PLE88_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOM75406.1"
                     /translation="MKSRRMVLFYLAGIFVSVCPEAAGTSEERLKIFLPRDITISNTA
                     PTLGQAAVLQGSEMLAAKAGSVALGYMAEPGQTLVISRNVVLSRLACSGIPLSSVTLL
                     GAEEVRIHCLARIVSGSQIAQEAADFLKENPPDKSACQFDILRSPADLVLADVSGDIE
                     FVCRLIENGSKSQCRVEVAAVRQGRTIGRREVVFRCRYPCRRVVTTRAIAKGELISPE
                     NTAIENGFSDSPEQSDWSVPYGQAAVRSIPARTVLTAQMTGAAKPPIAVKRNQSVAIR
                     IDNPGFTATASGKALQDGAAGALIKVQNVNSKIIILAKVNEDGTVEPVF"
     gene            12491..13105
                     /locus_tag="PLE88_02705"
     CDS             12491..13105
                     /locus_tag="PLE88_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOM75407.1"
                     /translation="MNQKINQIFLFLLAAVNAVQGGSIWARKDANAKNIYADDVARKI
                     GDVLTIVINETSNMTNTSEREMSRTTSRNNQFDGKIGVEHILTSIPAVNFGSGTTYTN
                     TFESEADSKNKRAFFDNITVVVVDIMPNGNLVVNGTCKRNISDDIQEIEVSGIVRPSD
                     ISYSNTVDSKRVANFNIIAKYKGVAAPYNKPGWLARILDVLWPF"
     gene            13129..14235
                     /locus_tag="PLE88_02710"
     CDS             13129..14235
                     /locus_tag="PLE88_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOM75408.1"
                     /translation="MKLQMITVLLIGTTLVLPAGAERIKDIVDIQGIRGNTLEGIGLV
                     VGLANTGDSTLPSRQMLTNILRRSGLVFSSNDLKSGNIAIVSVTAELGPFAREGSRLD
                     VDVSSIGDAKNLQGGTLLMTELYGADGQVYAVARGTVFIGGWSAAGENASITKNHPTV
                     GRIPGGAIVERAEVASFVESVSGNRFITLNLRNSDFTTAENISRAINEKYPNCAVTAD
                     AGTVRVAIPSFIGTAEIGGFIVDITQLEVAVDTPAVVVINERTGTIVVGKNVGISAVA
                     ISQGGLVIKVKEQQNVSQPTAPFTEGATTQVVNETLLGVEESDGYLVPMPRVVTVEEL
                     AKALNAIGATPRDMIAIFNALKKAGALQANLEIM"
     gene            14249..14587
                     /locus_tag="PLE88_02715"
     CDS             14249..14587
                     /locus_tag="PLE88_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75409.1"
                     /translation="MNPLPLSNNTRSPVPLTETDSLRQASQIQKEQFARDFESVFIEK
                     LLEEMQKTIGEWGLEQDSTAPQIQGLFQMHLARDAAAKGGIGLWKEIVQSLENMDSRK
                     GDIKEMDNRL"
     gene            14584..15111
                     /gene="flgN"
                     /locus_tag="PLE88_02720"
     CDS             14584..15111
                     /gene="flgN"
                     /locus_tag="PLE88_02720"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOM75410.1"
                     /translation="MNSDLYATELDNCIGRLLAVLDADIEYLENTILRLGELRCLVIK
                     HRYQPLEQMLQTIRTEYIRYRKNESQRQAICRKIGLLLGCPSDVMTLSDIAAHLPPDK
                     RTRLLERRVKLQTLTDLLRREYAGVQMLLADCARLNRMLLANLLGTGCAESKTYQSTG
                     TAERQTGTLFMDARC"
     gene            15128..17104
                     /gene="flgK"
                     /locus_tag="PLE88_02725"
     CDS             15128..17104
                     /gene="flgK"
                     /locus_tag="PLE88_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOM75411.1"
                     /translation="MTNYSIGLSGLTAAQRAFEAIGNNIANAATEGYHRQRLELSPAF
                     SQNPGSTVFIGGVNVEGITRIIDTLLEQELLRQRSVLASLSQETSALGTIEAAFGEFA
                     DEESGLNAAMDNFFTSLQNLSAHPSESIWQNQTVSAAQTLAGQFRYLGQMLTDMKSRL
                     KFQAETCADTINLLTSQVAELNSQIERIKMVGGDANSISDQRDRYIARLAELIAVETI
                     ERENGVIDVSSGGLPLVTGASANRIEAGYADTGRLGISILGASTYTTAIEGGTVGGLL
                     TLHNRIVAGIQEDLNCLANSIIQQINQYHAAGIGSAGSFTELSGISLKSDQLSDIGCV
                     TDGTFYIRLTNTVSGQVSRHAITVNAASGTLNDVAASISLIPGLRASVNSLNQLWIQA
                     DSNYTFDFLPGALPEPTMVSFADVSAPSVAVAGIYTGTENDTLRFTVRGDGSVGSGSL
                     QLEVRSRDGSGDLLTVLNIGAGYAAGKPLELGNGLTVALSVGSLSESSGDSFSIDVLA
                     DSDSSGFLAAAGLNVFFTGTSAADMAVSRRIEANPRQIAVSLGADGHDNINSLRLAAV
                     RDKTFSRLDSLTCSDFYRKMAAGIGEELSVRQTQQTTAETVLLDLTNRQSEISGVDIN
                     EQAAELLIFEQMFQAMAQYLNTIKTSLSNLMDIL"
     gene            17117..18316
                     /gene="flgL"
                     /locus_tag="PLE88_02730"
     CDS             17117..18316
                     /gene="flgL"
                     /locus_tag="PLE88_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOM75412.1"
                     /translation="MSGLNALYNNAAFALRLHAAAMAVLQEQASTGSRINRPSDDPAA
                     ASRLLGLSSQQKYLKAYISSIDSAVSTHEMASTVLQQMASALTESIVRLTQIVSGTYG
                     EGQEGQNARDRTAAEINDVLEQMVALANTKYVDQYIFGGNNTADIPYAVQRSGGEITA
                     VAYCGSSENRSIEAAPGVLASISLAGENLFSSDSPQTPVFYGSTGAKAGTGTSTIRGQ
                     VWLTVTFDGSSYCLSIDDGATQIQVPTEGDLSNIAVSDAAGRVLYVDASNLTSAGVEK
                     VTVPGTEDVFTALINLRDLLRNSRGFTDAMLADLIKHSSDSLESVKNSLVEKETLIGA
                     RVNFLASLKDNLENIQFNQQQEAAMIQEADIAQIAIDLSRRENLYQMSLLVSGKLMSM
                     SLLDFLK"
     gene            18474..19097
                     /locus_tag="PLE88_02735"
     CDS             18474..19097
                     /locus_tag="PLE88_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75413.1"
                     /translation="MNSTVHNIPADNKHDVTDGAILLERADYFLSVGSLEQARYYYEQ
                     LAVLCPDEPASYTGLGIVALRNHQLDDAQLAFRVACRLDPMHARAYEGLAMTAQQKGD
                     YETAFDMYLKCLELDPDNLSALLGLFQTSSRMGSFGRIIYYLEMYLKKHPDDVSVLFS
                     LATLYIKEDRFDTAQKLLRDILCLEPDNKDAKHLLEEVQQHLARVMS"
     gene            19097..19414
                     /locus_tag="PLE88_02740"
     CDS             19097..19414
                     /locus_tag="PLE88_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75414.1"
                     /translation="MTRQITKPLEDLFDQLDAVLNRMEYLIRKSDFDGVEMLCQKADL
                     IIARLDDMRLSGYPELKTRCKQVLGRYERLVLMLRSAQAAIYQQMHKLENGKKIIQAY
                     NGK"
     gene            19558..21948
                     /gene="fliD"
                     /locus_tag="PLE88_02745"
     CDS             19558..21948
                     /gene="fliD"
                     /locus_tag="PLE88_02745"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOM75415.1"
                     /translation="MGQITLTGLSTGVDTSAIIKQLMVIESRRLATYKVSKSNFEKQT
                     TALKDLRSKIAAVKSAASELANVRKMQLFSASSSNKDIVSVNASSEANPGSHTIQINQ
                     MAAADAWIQETSVFNYKTDYVGGGSFIYTYNGRQRIITAVAGETTLEDLVNLINNDEN
                     NPGVTASLLYQSGKYHLMLTGHEMGQDFQISIDSSTREVWKPDSNQPHSTFTSSQLNA
                     SLSTKIINLDQFGGTLGTADKIIISGKNHAGSNLPDTEISVTENTTLGHLIDAINRHF
                     DGTATARLENGQIWLTDNTCGSSGLEISLSYSGDAVLHLPSMTVSAEGGRDSESLALL
                     GSASFIQTQNASDAQIKIDGFPFSTANEVQTLSITGGIPAAGTFRLTLNGQTTDPIAY
                     NASAADVQAALLNLSGVEEGDIVCTGSALPAGPIAIAFGGRLAGMDVSQITVSDDGSL
                     DAGVVSIVETVKGSDGWIHRSSNHITDVLAGINLTLNDVTPADNPVKITVTRNTATLT
                     KKIQSFVAAYNDLMDELESKTEYNAETKTMGILSRDVAAAFLKTQASTPFTAIARGFL
                     ESADAFVQADGIGITLDGNGRMELDAEKFEKALAANYQAVLELLGAAKKGASSNAAVQ
                     VYNTSEKYTAPGIYHVKIEVDESGSIVSAKIKLSTESEYRDAVSWSGGIITFDSSFDG
                     TGNPLWPEHSLQLSVDAVAGTYGTDANPVVVRIKQGIFGNLNDMLTQLTKTNGQLDVS
                     AEALDQRISRMTAKIEKEEARLKRVEQRLTEKYSRLEKILTAMQQQMNAVSAMYNF"
     gene            complement(21956..23023)
                     /gene="flhB"
                     /locus_tag="PLE88_02750"
     CDS             complement(21956..23023)
                     /gene="flhB"
                     /locus_tag="PLE88_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOM75416.1"
                     /translation="MEDRPASEKTEQPTPKRLEKARQEGQVAQSQEFPAAVSLAAVVL
                     AGLALTPFLLNWFTTQLREGLYGESGVFADPATFVQYLNAKLSSALFVTAPLLAALLA
                     GAVLSSITVSGWNFSLQPLKPRLDQLDPVKGFEKLFTLRSLIKLAISAAEFLIISIVV
                     WRYLENRFDSLASLRWAWSAQLAAEFSKLLFGMLLRVCAAVLVIAAAEVVFQKWKYMM
                     DMKMTKQEVKQEQKDTEGSPELKLRIRRIQIAIARKRMLQEVPKADVIIVNPTHVAVA
                     LKYDPRRMSAPVLTAKGADHIAEKIREIARAYGVPIIRKPELARTIFATVKEGQPVPS
                     GLYTAVAEVLAMVYRLRRSRR"
     gene            complement(23063..23803)
                     /locus_tag="PLE88_02755"
     CDS             complement(23063..23803)
                     /locus_tag="PLE88_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOM75417.1"
                     /translation="MLKLLGFGAVLIRTSVFLSIVPIFGSQTVPVRIRAAAAILVSVS
                     LSIAIPSVIPTAGLSFLGMTVLIINEALYGLGLGLIAVLLFSAVKMGGQMIEREMGLA
                     MAEMLDPLSGETAEPLSILMEMIFILLFLAANGHHLLLLVISRSYEHFPVGQIPSYAL
                     LTEGIVQAGSTMLLAGLRLAAPMLAAFMLLLGVLAVFARMIPEMDILFISMPLRTGLG
                     LLIAGMMFPFIHSFIGELTDWMGKLLPL"
     gene            complement(23820..24089)
                     /locus_tag="PLE88_02760"
     CDS             complement(23820..24089)
                     /locus_tag="PLE88_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HOM75418.1"
                     /translation="MDENAVIFLGKQTLETALFVSAPVLITCIAVGVVITLFQAVTSI
                     RDMSLTVVPKLIAVGIVILLSGHWALERMIRFTSDIFGYIQQIGR"
     gene            complement(24109..24915)
                     /gene="fliP"
                     /locus_tag="PLE88_02765"
     CDS             complement(24109..24915)
                     /gene="fliP"
                     /locus_tag="PLE88_02765"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOM75419.1"
                     /translation="MYYKQIGFLLLIVLAINTPAVCWAQENPTSNDGRRPAAVPPIGD
                     LLNAVDKATSAGAESKEWSTPVKLVIIFTGLAVLPSLMAMTTSFTRIVIVLSFVRRAL
                     GTQTIPPNIAIMGLALFLTLFTMAPTFSIINETSIQPYLKDQIDFAAASEQAKEAMKA
                     FMLRQCRQADLALFVQMAGIEAPASPAMLELHIVIPAFIISEFRTGFEIGCLLFIPFL
                     VVDLVISSVLLSAGMMMLPPVMISLPFKLILFILVDGWGLLAKSLGLGFH"
     gene            complement(24905..25315)
                     /locus_tag="PLE88_02770"
     CDS             complement(24905..25315)
                     /locus_tag="PLE88_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOM75420.1"
                     /translation="MSNNTVSAVEKQNSSNIELWTQQAQKSGNPDENLSSALHRMLIG
                     TLVVVVLGIAALMASKKVMPRLTPVHGKQIKVVETVYLGSRRTLHLLEINGRQILIGC
                     TNDQICRLSDSFDKVPSNVSPAVQTKLMDTPNVL"
     gene            complement(25553..26281)
                     /locus_tag="PLE88_02775"
     CDS             complement(25553..26281)
                     /locus_tag="PLE88_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOM75421.1"
                     /translation="MLKSRKKQESGSPNVPAYIVTFSDMVTLLLTFFVMLLSLAQVRD
                     PELFNRSRNAFIAHIDCYGLGMLTGKQLVPELGELKIKYQTPAPDEKPPQRIIDSKEE
                     DLRRLFQKVIKSMHAVRTEIEIRPSDYTVTSIVFPRGQAALKDADQKYLSQFAMHLQQ
                     KAAETIEIYVLGLAGDESTEKNQWIISSLRAQNVADFLRESGIQCPVYAWGAGAGGHW
                     LSQEKCDSEQTHILLAVRRVDKIQ"
     gene            complement(26283..26975)
                     /locus_tag="PLE88_02780"
     CDS             complement(26283..26975)
                     /locus_tag="PLE88_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOM75422.1"
                     /translation="MARDKQNADAAPEAGAPEWMVTFSDCMTLLLTFFVLLLSFSSFD
                     NQDDYLKMTSSMAQQFSFGQQSQTEKDSVVFIREQVPNYELDWGSEKKTLEEKEKNRS
                     RNTTSPADYLKYKKFVMPSSEAFWGKGMVLSVQGKQALYNLALFLKEFPNHLIMVSES
                     GSSSDELTPNYGIQRAGTVIDYLTKEHGLDPRRFCLSAAGTMDYAVPSDPETPSPVSE
                     RMLEIVMLPGGR"
     gene            complement(26975..27745)
                     /locus_tag="PLE88_02785"
     CDS             complement(26975..27745)
                     /locus_tag="PLE88_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOM75423.1"
                     /translation="MDIATIIGVLLGLVFILGAIAVGAGAKMFFNVPSLIITIGGMLC
                     ATLIHFSLSQFLSIFSIIKKTIVSKLPAQADIIQKMVNYAAINRRDGALALEPEIHKL
                     DDHFFAKGLQMLVDGQNEETIRDYLTLEIEYLQERHSVGKKILEFMGASAPAFGMIGT
                     LIGLIQMLSNLSDPSGLGVGMATALVTTFYGAFASNLIFIPLAGKLGIYSKTETLLKE
                     MITEGLCAIAQGENPTLVRERMQAFMSHSSRKDIKAVI"
     gene            complement(27750..28448)
                     /locus_tag="PLE88_02790"
     CDS             complement(27750..28448)
                     /locus_tag="PLE88_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75424.1"
                     /translation="MKLKLAAFTAGIGLVGFGSMFAVGWITKPAAHLPAAGAQTAGTG
                     EQEKTSTMSSEAAVGIIQEALAGDPSRIKMTDQQLQTLTYEIREKIREYNSKLRNLET
                     QEKRLQAAQESLKKDIEEMEQLRVELAAAAAALKEQQDRLMQSRLVIEQNEKENLTSI
                     AAAYDKMDAESAARILMAMVKNPSAAAGADDAVKILYYMAERTKAKTLAAIAEAEPAV
                     SVYICQRLKRLSAE"
     gene            complement(28483..28947)
                     /locus_tag="PLE88_02795"
     CDS             complement(28483..28947)
                     /locus_tag="PLE88_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75425.1"
                     /translation="MSTFRWRLQRVLDIRQKQMQLLRAELSALTVLLGRKRCELMLQK
                     KMLENLIEKLQSDPPRRRLEQQAFFLRYSEANDSAIRRLEGEIKQLSEQQKKKEAELA
                     RVEQFTDALQRLRTRAEEEFAQKQEISEQKQQDEETAFRFARLMQLQQAAPE"
     gene            complement(28947..30299)
                     /locus_tag="PLE88_02800"
     CDS             complement(28947..30299)
                     /locus_tag="PLE88_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOM75426.1"
                     /translation="MLSVLQNINLGSCHGIVVRVAGLTIESSGPKVGLGELCYIHLRS
                     GKRIPAEVVGFQQERLILLPLEHIEGISPGDRVTARCTPRYINVSEDILGRVLNGLGE
                     PIDGRGPLVGSEQKALDSESPPALQREKITHPLALGIRSIDGLLTCGRGQRVGIFAGS
                     GVGKSVLLGEIANSSDADVNVVALIGERGREVREFLETNLGAKGLSRSVVVVSASDSP
                     PIQRVKAAFTAVAIAEYFRDKGLHVLFMMDSFTRFAQAQRQIGLAAGEPPAAKGYCPS
                     VFSLMPRLVERLGCSDRGSITGILTVLAENDDMNDPVADSARSLLDGHIVLSRKLADR
                     GHYPAVDILASVSRLMPMVASEQHKLAARRLKELYATYLDAEDLINIGIFAAGSNRRI
                     DGAVALIDRVRDFLVQRVGDRTSFEQTIRQMTEITDRWYELLSSDSDKNRQSVSTAVK
                     "
     gene            complement(30393..30962)
                     /locus_tag="PLE88_02805"
     CDS             complement(30393..30962)
                     /locus_tag="PLE88_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOM75427.1"
                     /translation="MFSALVISVDKPIAAVKLADSQGAASTEISAGGPSGPACDACRK
                     SLDRQKQLYQNACLIFQEAAEQMHRFCDELLAGHQEAIARLSVEIARKILMCKISQGD
                     YDIEARVREALHAVPKCGSITVRLNPQDLSDLQSLQQEHPSAAEGIQLTADASLGRAE
                     CVVESPRGIVSYLINEQLEQVGKALAKTH"
     gene            complement(30967..31944)
                     /locus_tag="PLE88_02810"
     CDS             complement(30967..31944)
                     /locus_tag="PLE88_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOM75428.1"
                     /translation="MSGKQKAALLLMGLDAATAAELLKGVDPQTVQELAVEVAYLDAS
                     GFSSISQTTEVARQFYLSLENSRGLDVQNFLATMLKMTVGPEKAERIQAQIRDLLQKR
                     DPFIAIRHADSKTLASILAGEHPQTVSVVLAELPARKSSEVLALLDEPLRLKVVTRMA
                     AAEPVLADTKMRIAQMVCRRLESAQGDTAGLEKAPRKVALMLRNLSKEIRDGLISSIA
                     QKDAEAGRLISEQMLLWEDIPDIADRSLQEALRQADLKKLALALVSADEGVARKIKAN
                     MSERAAAMLDEETALLASPRKADIQQAREDITALLRQMNEKGELNFNEE"
     gene            complement(31954..33474)
                     /gene="fliF"
                     /locus_tag="PLE88_02815"
     CDS             complement(31954..33474)
                     /gene="fliF"
                     /locus_tag="PLE88_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOM75429.1"
                     /translation="MSFFQKTVALWQAIGIVQRALLVSMVLAVLLVSGLFAYWARMPE
                     MRVLYSGLDPEEAAKITDKISEKGIVYKLSSAGTTIYVPQQHVSQLRLDMAKEGLPGT
                     GQKGYSLFDDEKVGVSPFVQNVNLKRALQEELAKSIQMIDGVTHARVHIVCPENNYFS
                     LSKTQTSASVILRLRPGCMLSAQNVAAICHIVTGSVEGLKAENVTIVDSQGRLLSRQT
                     DDALPGGAGTAADYRERIEHNLETKVQDMLTAVLGPGRASVKVSAEVDMTSINTAKET
                     YDEGKRVATKEEITNESQIEGAKAAGEGESPAGNTKKNETIITEYAVPKTVQQTVALA
                     GEIKSLTVAAFVDLTQESKAAGGDEAAPAASEEPIMPVSEIEDIIRKAVGPKLAADGL
                     KVVNVKFNRPADVPAEEETRRLDYIQIARQSSLGIMAICALIALKIFGGAGKKAFAQP
                     SSPLGAASSGSFAGLPAASPDMSESMMLRRQIAVSLRNNPEQVKQLFSSWLQEKGR"
     gene            complement(33528..33848)
                     /locus_tag="PLE88_02820"
     CDS             complement(33528..33848)
                     /locus_tag="PLE88_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOM75430.1"
                     /translation="MIQFNPSIPSSFPNSAAGNIRPAGTKPADSGSVFQDAVKAAQAE
                     LNKVDDLVRASDVSIRDLLAGRNEDITAVVSDVARADMSFKLLVGVRNKLVEAYKETM
                     NMPL"
     gene            complement(33862..34263)
                     /gene="flgC"
                     /locus_tag="PLE88_02825"
     CDS             complement(33862..34263)
                     /gene="flgC"
                     /locus_tag="PLE88_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOM75431.1"
                     /translation="MNVNGLGTIDIAVSGLRAQGRHMELISSNVANAQSTDNGQGQPY
                     RRVEAILKTDGKGIAGVKIEKIAEDMSDFPRILRPGHPDADEQGYVAMPNVDVPTEMM
                     NLNTAVRTYQANAAVLKRYQQMVETTIELLR"
     gene            complement(34315..34674)
                     /gene="flgB"
                     /locus_tag="PLE88_02830"
     CDS             complement(34315..34674)
                     /gene="flgB"
                     /locus_tag="PLE88_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOM75432.1"
                     /translation="MTKIDTITELLNAGLRAETLRQKAIAGNVANLETPGYRRIDVKF
                     EELLAKALSASDAADASAVEPQIYQPETTPVQSNGNDVSLEGEVGQMVKNTLRHKTYI
                     RLLQKKYAQLDLAMNIK"
     gene            35416..36717
                     /locus_tag="PLE88_02835"
     CDS             35416..36717
                     /locus_tag="PLE88_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOM75433.1"
                     /translation="MADIPIPIAAPNAVPASLDVAAVQPVEQTDELSTLNNYSKSGKQ
                     IAAEALTRQIPDSSPAVKTVISNSSKPVKDWMPVVSDSVPTTDGSRSDNLQKPLFTGY
                     ESRLTAGNDRMPAMHSDSQSARLLDSPADQESTDQMKSAGLSDDITQLHRIRFKHNAT
                     PDKIAPEKMVQDSVHTEQQKQGTPSKAVNAESDAHPSVLIESSKSEDENNLILAKTAV
                     QKLNIAAEQIKPLYAEKSQTAKDEFRLKPASDNHNLIQGSDLVAGKNPVQTEDGLPRP
                     VQSIHSFLGLGRQIQESVAVFYRHGMRQIVIRLDPPELGRVTIKFIEHHNDITGILHV
                     EKSQTKQEIQQILPELLLNLQSSDVSIKKLEVALSSQQNNVAQEHSARDNGSTGQEYQ
                     HNTVGPSSEEWSSQNDAGPEHGDGLSQMRLEVSDKTINLLA"
     gene            37017..38285
                     /locus_tag="PLE88_02840"
     CDS             37017..38285
                     /locus_tag="PLE88_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOM75434.1"
                     /translation="MLAIKNNIMAGNAARHLGKAYDSLAKSVERLSSGLRINSSRDDA
                     AGLAVRELMRADIAVLQQGARNAQDGISMLQTMEGAMAVIDDNLVRMKELAEQAATGS
                     YSDAQRAIMNAEFAEMADEIDRIAEATKFNDIAMLNSSTGSVSIHVGTSTTIDINKVN
                     MTKSGLGINTGVEVDEVIFKGTESATTDDFLTVADNAVNLVIQFSNSADGTSEDAITI
                     DLATGSYSMAAVVSAINAQTQALGYAPDGTDKSYNMASIVTNDDGTYSLKLSSRDEAD
                     AVSIQVVSATTDQHGTVSGLGGTATTSTNAGADLNTDNYDEAQVAGGVNIATAAAAAE
                     ALATITAAINTKDQARASFGYKMNRLESTIEILDIQAENLSTAESRISDVDVATEMAA
                     LTRTQVLSQAGISMLAQANQMPQMALQLLR"
     gene            38390..38791
                     /gene="fliS"
                     /locus_tag="PLE88_02845"
     CDS             38390..38791
                     /gene="fliS"
                     /locus_tag="PLE88_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOM75435.1"
                     /translation="MKGLETYQEIAVTTQSGGRLVVMLYDGAIKFLKRAVNELNAGNM
                     AEKGLYINKALDIINELNNVLDMDAGGEIAANLRTLYLFMARHLNSANIKKDPQMIGD
                     VISLLEELNQGWKAITRNSTLESEISRRSAE"
     gene            complement(38804..40273)
                     /locus_tag="PLE88_02850"
     CDS             complement(38804..40273)
                     /locus_tag="PLE88_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOM75436.1"
                     /translation="MFAVAALVLTAFFLIPLPNKLLDILWICLFCLAGAIAVICAAAK
                     HSFELIGFTALVRGVILLLVAVQSSTARCIIEDRQAGVLFSWAEAALSFSQPLAILMV
                     CLFLSMAAVFAIFVFSQKIVLSSTTYFRRVLPLKQMGIETDLRLGAVDEKQALVLAGR
                     VLLESRFFAGMHSSAVLMRTAGAVGIFILLACLLVPVYSARHYPAAVMGFVPDAAALS
                     GFSILPLLTMAAACGFLAGKNSLMMRLNGAEATISAIQTNLVGPGSESDMRPSAIQPP
                     PVKQPIEEHIVEFEPQEPAVIGSNPVWPATPCRDPKAYYQQLVKLIGKGNTQPRLIGL
                     VSQSPGTLPITVAVNAAIGLAQQKRRILLADTDNQRNAAARVFDMEPELIQRKIVPSG
                     LDNLWVCCVPPPKLNAFLGKTDLLGHFDTILFYMPQAHTLDRIETDQPIMGVFYFTAD
                     ETAGIGLQDIQMPAFCHWICPVPSLQSVLSKQSPRKREG"
     gene            40742..41830
                     /locus_tag="PLE88_02855"
     CDS             40742..41830
                     /locus_tag="PLE88_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM75437.1"
                     /translation="MSIVEVAKLAGVSHTTVSRVLNKSGKVNTATADKVRRAMEQIGY
                     VPKPPHLRRGPAAGSNRGFTTKNLAFLTVSETLRILSTSPFLVNLVHGLQEASSDNGL
                     SLYQGVLSADRPLPPFIARGEVDGLIIYPGLESVPEEYVKAIQRYPVVFVLSGRDKYF
                     LGDRVMCNHEHIGKIAAEYLIGKGRQRPVFFDLKKSPNYHQELFMGRWNRFEEVCRQA
                     GIQAKRVEIPLTSEETLFDGIKVQKTLTTVVENVFVNCSSDTRPDGVFVVFDSLTAVL
                     YPVLQSCGIRIGQDADIISCNNEVSLLAGLHPRPATIDLQPEIIGRRAFEQLRYRIQN
                     PKDQTRVTLEILPKLIEGFPQWQMEKQE"
     gene            42116..>43598
                     /locus_tag="PLE88_02860"
     CDS             42116..>43598
                     /locus_tag="PLE88_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75438.1"
                     /translation="MKWKCLCWMVAAFVPVAAADILYTAYINADTGYLYRYDDNLNLI
                     WQTSNQRGIHRFAVSPINGNIYAGYDGTDRWVRQFDRQTGALIGTTVPTISGLSSNHV
                     NDVAFGHDWNLDGVPDLWIVTRTSLLVFNGAQTVGSGAATELARWTVADSSSTVYDGT
                     GGSAILFGPDITGDGVAELYVGKGGNNSYGRINVYNIAASTVDNLVKAATYSAGNTRD
                     IENLLFGPDVFGNDGHLDLVGVSARNYQLRCFNFLTGADMGVIEEGLNSRYYPVSLAL
                     MPNGSFLLGTRMKTELDPLWVSGAETSGGNLVRLDRVQGPAVAYTPTLLMLSPLGGTN
                     DYRFVDVLYLEEKAAYAPVPAPGKRVPLDLAQISWTNPEPNEPSGVITCDVWFSANYP
                     EYLPFPDPNLLKIPDPNDRTAWWLENRNFTNYAAKVVSNQPINSLNLASVTTLPLTYG
                     QTYYWRVDTRDSSNPEAGTTVGPVWMFTAYNSAPQVDAGPTIYT"
     assembly_gap    43599..43608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <43609..43976
                     /locus_tag="PLE88_02865"
     CDS             <43609..43976
                     /locus_tag="PLE88_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75439.1"
                     /translation="TVQMAPVVSDDGRPSPPGKLTFLWSLQSGTSEAVTINSPEVQDT
                     TITVTAAGTYVFLLTVSDSELNNADTVTVVVSADACAAAKSIPGYVRRAGDINDDCQV
                     NIADLQLLAADWLNSTALK"
     gene            44094..45167
                     /locus_tag="PLE88_02870"
     CDS             44094..45167
                     /locus_tag="PLE88_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75440.1"
                     /translation="MRKWKVVLMTIGILLMLQTVPAATVDFDGTAGDGTSWEDPGNWS
                     GGPTGATGLPGSGDYVVISGDIGGETINMDAGTWQYFVDNSLLTGGSAGEYRTQSIRL
                     GWTHTSTLNVDIGDGNIWRTTNSTLQGVGSASGSNGTWNIYSGDICIESSSFRIAEKA
                     GSAGLVNIAGSANTRLIIHRETGGVSMQVGTGGAGIFQIDSGKFRTRAGVTVGSNGLF
                     RVLGSDVTEVSIGDESTLDGNWTQNAGGILEFGIDAGGVTPIVIADKGGSGTFAIFDD
                     GSLLNLHFLDAPIAGTWTLMEVKNGFITDNGLMLAAGVDPGWSFSIDNSGPNGLLTAA
                     YVPEPAAATLLGFGSLILLRKRK"
     gene            45242..46018
                     /locus_tag="PLE88_02875"
     CDS             45242..46018
                     /locus_tag="PLE88_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75441.1"
                     /translation="MRCRISRQGFTLIELLVVIAIIGLLLSIILPSLRKVKESARLLV
                     CATNLRSIHAALAMYAENNNDKVSDPRGDTTASPDPRPNPAVYWKGYAYDRWCRKWYL
                     RFYDYLETPEVYVCPSWTKREGDSYIAYAVGDDIYYVTYTGNEYILSLREHESQSGGV
                     RKPHEWKYTELVRKAVGNNPMALFLADGFYEVNGWGNWKAWQREGVMAPGGSGRASYR
                     HGRRANFLVADGRIGCLSMEQVDSWSGHGSYDEFKPSELK"
     gene            46063..48411
                     /locus_tag="PLE88_02880"
     CDS             46063..48411
                     /locus_tag="PLE88_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75442.1"
                     /translation="MRRYCILLAFLAMCGLCRGDVLDGIVFGDTHSESAHSVLAPMTA
                     IETGGLGQPCRRLSASGSITFTLACDPVRQNYVTVKLWGSDVVNGTQYLYLYDAAGRI
                     GTYQDDWPEIAVWTSQPPFPGRFVYSTYLIPKQLTSAKTQVTLTLADAQLGIYGVYSH
                     VDAFFNPLGEVHGQHPGPGPVRPAGWRSPYDQLVYQANLGIAEFLTWQKYGPAWNAAV
                     AAGNAPAVITGAITFDGRGGSPSWTVQQWKEDIYARFTGGNLVCLQALEAYAVAYNSP
                     WSNYYRNPELLDRIVKGLDFFRIAQGANGAFGNPWGLLWIGGPNRVNGAHCLEGFGVH
                     ALAEAFLQIHSYISPAVMNELVDEDDNPDTPPVTRKTSYINLFKGIINHMLTDRGHAP
                     NQDRAQVHAMYKSNQCLKILSPAEAWPESTARQWIYEAIGALPCPVYGGYWESQKGLA
                     LEVHGTINGGYCGSYGTAAISFIYPYGVMTGGQDALVNSIFSKEVDAFANYLYQDNDS
                     DGFAGLVNESVISWRPNKYPGVQFPSPGAFNGLAYAALYLNNQNAVRILQKYLENRLL
                     YTLDCSASAVAAHYEDNTIETIKMVNLFKQVEALAPTNARLPMEAGDTFAWADEQAAG
                     LAVYHNGSRLYMTLNWRHDYCSGCSRSAANAIANNIARIHYTTDTIDRIANVQMKTPY
                     GLFKLYVCRYGDYLIAMNLSDTESFPVDTSDLPWTDAVDLIGKVRMNLNAGLVIPPRS
                     TMILYPGRCMDYSAGDINRDCVVNLDDFSILAAQWLDNTNLQ"
     gene            48591..>50495
                     /locus_tag="PLE88_02885"
     CDS             48591..>50495
                     /locus_tag="PLE88_02885"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM75443.1"
                     /translation="MRQKVQIVYLIVIGVSVGLLHAAFHHPGLLHSRQQLDFIKNAVA
                     AGQQPWLNGYKQLAAHPQASYQYTVNGGYQTVGRGRNRTDNVHKAEFDADCNAAHYNA
                     LMWYLTDDRRHADKAIEILNAYARTLKEIIGTDKILMASLNGAKLTYAAELMRYTNAG
                     WDSADSQQFETLLLTVFYPVIRDFAPFANGNWSTGCVKTMMAIGVFCERQDIFDRAAD
                     WYRSGTDNGSVLHYISENGQCQESGRDQQHAQLGLAHLAEACEIAWNQGLDLYGWADN
                     RLLKGFEYTARYNLGYEVPFVPLTDRTGRYYHSAISEAGRGNLRPVWEMVYNHYQKRR
                     GLDCPYVRQAAEKLRPEGAGPYADCCGFGTLLFTLPADRTQSRQSDSAGFENWTGFKH
                     CEQAAEGGQAVFTLTIPGRAECVTNRWIELGGWEMPGLDAIPESRLNAADLREYQSVC
                     FTAVLSDDRSVRLRFSVWPHAVSRGEYPKEVASTVVVSGKGPHDIVLPLEQFEYPKAM
                     GAFWKFIQKISLSAEYVHPADAGRLQISGLRLSRGELIKLSAPVKSKPCQPGQTVQYG
                     LTAENLTDRRLQVRLFTESYGWEALQPELSTLVLQLEPHASEMVSVSVTMTDDVAPGG
                     RQTHMITAVAD"
BASE COUNT        12562 a        12608 c        12842 g        12473 t
ORIGIN      
        1 ggtataagcg ccggtaaatg cgcctttttc gtgaccgaac agctcgcttt ccagaagtgt
       61 atcgctcaaa gcggcgcagt tgacctgcag gtaaggcccg ccggcccgcc ggctggagta
      121 atgaataagc gaagctatca gctctttgcc ggttccgctt tcgccgctga ttaaaacggg
      181 tatcgacgtg gctgccactt ttttagcaag ttcgacagtc tgcagaaggc gtggactttt
      241 tccgatgatc ggatagccct cttccccgga tgccggccaa tgcgctgcgg caacagcctg
      301 attcggaaca agtgtgtcca gcacggcttc aatcgcactt cgctccagcg gcttcagcag
      361 aaaacggcgg cagccgaccg ggacggcctg aacggccata tccagcagca tctgcggctg
      421 aaaagttccg gtttgaattt gacctgtcag cacaagcggc aattccggaa atgctgaatg
      481 gatttggcga ataaagcgaa agctctcggc cggctggtca atcggcacat tcgcaaaaat
      541 aaggtcagcc ggacttttgt ccaacattgc agacgcctgc cgaaggtccg aggccacgag
      601 gccgcggatg cctctgcggg ccagtacctc cagtatctga cgcaccgcat cctgattgtc
      661 atcgataata agcacggctg cttctgcggt tttcattttg attcaatttc ttctgccgtt
      721 gacaagggat tctgctcaat cagtttccgc ggctgcacct gcaggttcca ttctacccac
      781 atgctttttg ttttagccag cgctgtccat ggcgaattcg gatagtcctg tatgaccctc
      841 ctgtagctgt ccagcgcctc ggccggattg tcagcccgca gacagtcgcc cagccgcagc
      901 agcagccaag ctttgtcgtc tgcggaagtc ggctgactgt tgagccggtt taacgcttct
      961 ttataacaga ttgcggcctc tttaaggcat ccgccctcat atagaatttc acccagttga
     1021 tatggatttt tcagtgcgtt tggatgctgg gcagattggc ggaataacgc cagagtttgg
     1081 gcagaaatcc tgccgtctgc ggcagcggtt tttacgatgc cggcatcgtc tgcttgctgg
     1141 ggttctccgc cggcggattt ttccgcaggt ttttctgctg gagccggaac cgatacagcg
     1201 gctgtcggct cggcaggcat cttaggtttg atttctatcg agcggattcg ccggataagt
     1261 ccttcaagtt cttcttttgt cggatttgaa gaaaaagagg gtgatgcact tatccgtgcc
     1321 tgccatacct gctgctgcgc tgtcagggct gctgttcgag ggggcggaag caaatggatg
     1381 aggtcgctgg atgccgcttg tttatcgatg gcagcggtct tttctgtaat cgtaaccgta
     1441 tttggttcgg cagtcagatt cggcatttct tttgtttcaa tctgtaaatc attttctaac
     1501 agctggaaaa aatctgactc ctgtgccaaa ttttgcccca agccggtccg gctggcagct
     1561 gctgtcatca gaactgccgc caaaacagct gccgacagac agcaattctt cggatggtgc
     1621 tgcatcctga tctgtcggtc ctcggagaat ggtcgaagtg catccatcat ttgttctgcc
     1681 ccagcaattc caaaaccgca aggtcatagt gtttctgtgt ctgatatatg cctgcctgta
     1741 tttcggcggc ctgctgacgg atggagtcag caggatttaa gcttatcagc tgtgaacaaa
     1801 cagcagatgc ttgcgtatgc agccctcgat ttacacacac ctgcgctagc cgcagcattg
     1861 tctgatgcat aagcggaccg gattggaccc actccagcgt ttgcatcagt tttttatagg
     1921 cataatccca attgccttgt tccatatagc attggcttag ttcgctgccg accattgctt
     1981 taagcgaatt atccagcaga tagctttctt tatcgttaag aaaagcaatc gccttatcgg
     2041 ttaatcccat gccccggaat acccggcttt taagcaccag cagatgaacc atctcttgtt
     2101 gcgtcaaatg ccggcggagg gcctcattta gaatatccag cgattgaata aagcgttcct
     2161 gcacgatgta ctcttccacc accgcggtga cagcttccac ataatctgcc tgtgaaagat
     2221 tccccgaaag cgctaacagc agtgcatcgg cggcagattt ggagtttccc tgcctgcgaa
     2281 gcattttgcc caacaatagg caggcctgat gaaattgacg gtcttgccgt gcattttcat
     2341 ctgtaatata ccggttgaac cataattcgg cattttcgaa atccccatgc tgataaaaaa
     2401 gctgtccggc tgtcaaagcc gcagacaaac ggctttctgg agaccggtta agataataca
     2461 ccctgctgta taatttggcc gcctccgatg cagatccttt tttaacggct gcatctgcca
     2521 gaagcagata aacctgttcg atattgggat aatcaggata ttgttcggct gtatccttta
     2581 agagggtgta agtgccggcg taatcataca gatctgcctt gtgcttggct gctcttacta
     2641 atgcataggg tgccaattcg gagttgggaa agcgatgggc tgccgttagg aattcagcaa
     2701 cggcttgatt tgtccggccg ctgacggctt ctaacagtcc catcgtaaaa tggacattgg
     2761 catgaccggc atcttcatca taaactacca gaaagcgctg ccagcatgac aaggcatctt
     2821 ttgccagcag gtcaatctgt tctgaaacac gattgtagtc ggctggatta aagagtctga
     2881 tttcggtttg cgtatctggt acggctgcca agccggcaca gccggcggca atttttacac
     2941 agtctggttc tgttgctgac cttaaataga ggcttacggg tcggttgtga atgcctgttt
     3001 tatcggacgc aatgtcccag tatacattac tgcctgtatt tactgcaaaa cggcttgtca
     3061 attccccaat tgatgttccg ttactgatta cagtccagcg gcgaacgggc tgtcggtcag
     3121 cgatttcaat caccggtcct gtcaatgccc gactgaactc agcagcaccg gtattcagca
     3181 ccgcacaaag tgactgttca tctatttcag acaaaatatc caatcccggc caattccaca
     3241 atgccgctgg aagctcttta tctgcgtccg ataatgccag cacctgacgg ctgacggcct
     3301 gtgctgcaag gaaatcacaa gttttccgca gccggctgaa tccgcttagc ggcggatgga
     3361 cacaatccag cagtgccaga gcctgatatg ctcttgaacg ggcagacagg tattggtgat
     3421 tgcgcatttc tgcctgacac aagtaataat tggctaatat ccgtatcaaa ggcaggtggc
     3481 tgacctgcag atttttcagc agcccataag attgctcgct gttgccttct ttatgaaaac
     3541 ataaagccgt cttgagtttc acaaattcct gcaatccatc atcctcgttt ttctcaagcc
     3601 catccagaat cttgctgtat attgcacgag cataggcata attgccttga cgataataat
     3661 cctgtgctat tttaagagat attggatgct catcaggcaa aattgcaggt ttttgcaaag
     3721 cagccgtttg gccgacttta ttttgaccat catcggtctg cccccatgct ctcatccgaa
     3781 tagagtgaca gacccataga tatgtcaaaa tctcgtagat cccgataacc aatacgctca
     3841 agatcactcc tgctgccagc tgtttgagaa taccaatccg tcggctttgg tttgttccaa
     3901 ttgccggata gagctgccgt tggccggcgg cagcggcgcc atgtttgatt tcctcacgga
     3961 gtattgcact gttgtcagaa acagtgtcat ccggcagccc agagagtaat tcatcgctgg
     4021 gggaaacggt gccggcgtca tccttggcat ccgacaagga ctcactttga gccagcttgc
     4081 gaatatcttt aatccgctgc gaccattctt ggcctaaggc gtcaagattg ttgttatgaa
     4141 tctggtccgt catcgtttca gatgctgcat ttgttctgtt tattgaggcg cataaacccg
     4201 acacttatcc agtgcaacaa ttgtgccata gcagccgagg cgctgcagac tatcaaaaaa
     4261 atatcttatc tgtaaacagt gggcgaattt ttgggttcct gaactgtata gcagcggcgg
     4321 taggtgtcaa attcttaaac aggatcatta cactgctgtg tgatgcggat ggctgcgatg
     4381 atataggagc cgcctactca gacggaacag tttcgttcgg aaagaaccga ttttgcagcg
     4441 aaacagtccc agaatcgttg gcagtaatgc ccgttaagct atggcagcag attcgggcat
     4501 caaatctgac ggattgatag ttgtgttgtt ctgctcatcc gattctgcat caacaatatt
     4561 gtgctgtttc agctttgcat ataaggttgc ccggctgatg cctagcagtt cggccgcttt
     4621 tgtcttgcgt ccgtttgtct ggcgaagaac acgggcaatg agttcctgtt cggcctttgc
     4681 cagcgaataa tctttgattt tttcactgct gtcagagtta aagaagtctt tcggctcatg
     4741 gggattgata ataatgctgt taaggccgat tttgtctgtg gtttcgagaa gaacagcacg
     4801 ctcgataaca ttccgcagtt cacgaacatt gcccggccag tcgtatgctt ccagcgcttc
     4861 gagtgccagt tgagtaatcg atagattcca tttggctttt tcaggacata tggtctgtgt
     4921 gcgaataaaa tactcggcca gcagccggat atcgctgcgg cgtgacggtg accgcagagg
     4981 gcttagatgg attgggaaaa tacacagccg gtggtacaaa tcggcacgaa accgcccgtt
     5041 gctggcctcc tgccgtaaat cgcgattgct ggaggcaata atggtagccc ggcagacgat
     5101 ttcccgcgtg cccccgaccc ggcgaaactt cttttcctga ataacacgaa gcagtttggc
     5161 ctgtaaatcc agcggcattt cgctgatttc atccagaaaa atactgccgt caccggccat
     5221 ttccaatagc ccgatctttt cacggtcagc gctggtaaaa gcaccttttt cgtggccgaa
     5281 aagctcgctt tcaagcaggt tggcgttcaa cgatgcacaa tttacggcaa tcatgtcccg
     5341 gccgggcgag cgctgcaagt gtatcgcata ggctgctaat tccttgcctg ttcccatttc
     5401 gccggtaatc aaaatcggat tgcaggcact ggatccaact actttaatca tcttaagggc
     5461 ataaaggaca gcagggcttt ttcctgcaag tcctgctgaa gcggctatat catcggcaaa
     5521 aaaatcgccg ttattatagg tagccagcgg caatttgtgt ttaatcagct ccataatcct
     5581 aatcagcgag gactgatcgc cggggctgtt taaatagtag acattatctt tttttatagg
     5641 accacaggaa ttttgttctc cttctttgcc ggtacctaca attacaacag gtatattggc
     5701 aatatggtgg gctgcctgct gaaaaccgtc gatttctgcg gcggtaaaat gagtgccgaa
     5761 tagaatgaga tctggactga accggcgcgt caattcaatg gcttctgaga tgtcatcggc
     5821 ggcatataca ttttttgcaa atgtctgggc aagcgtctga atttccctct gacggccgat
     5881 aataacgata ttgtttatac tcatagcgtc ttccgatgtc tgattctgtt atgaattccc
     5941 atccttgaga ttcatataaa cacaacgatt tttcaaagag attagaaaaa ccctggagaa
     6001 ataagcgggt aaaacggcag ataattgagc aatccgtctg ggatcactgc ctttaagccg
     6061 gttttataga acccggactc cagaatactt attttttatg tcgtaattta caacctcaaa
     6121 actttaataa tttttatggg acgatttttt aattataaga ctatagaaaa caatgtttta
     6181 agcttcaatc agaccagtgc ggcaaacgta ttttgataat gctgcttata cagagcaggt
     6241 gtattgcaga aaggaaattt tttccggcag ggaaaatctt tttgtctcta agcgatacaa
     6301 gttaggagtt cctttctctg tgtccttgct catgcaaatt ctttataacg tctattaaaa
     6361 taaaacgtta tgctttttgc ggatattcct gcggctggtt aatggcacgg aatttgctca
     6421 tttgttgtaa atgtgcggct ggatgctgat tcagccgttt gctctttgga cccaagatct
     6481 gactggaaag gtaaagaata atgagcgaag tcttgtcggc aagctcgctt caaattgatt
     6541 atatgaagct gcttactgcc caattacaga atcaaaatcc gctggagccg ctggataatg
     6601 cggatatgac agcccagctg gctcaatttt cgcagttatc ccagttggaa aagatgaata
     6661 cggccttcgc tgatgtcctc aaaagtgtgc agcggcaata tgcctcatca ttgataggaa
     6721 aagaagtttc atttgtaggc gaaaccggcg acggcagcgt caatctcatc agcggacagg
     6781 ttgaccagat tgccaatatt gacggaaaac ttctgctggg tgtgggggat tataccctga
     6841 ccttggatga tattttgcgt gtaaaaacgt cagtcgagtc agcagccgat tccgatagat
     6901 aacaaagtgt gaacccgaaa aacaggagat aaacaatgag ttcggcacta tccgcaggcg
     6961 taacgggact gcaggcgcat caaaaaatgc tcgatattgc aggaaacaac cttgccaatg
     7021 taaataccac cgcattcaag gccagccgca tcacattttc cgagttgtta agtgagactg
     7081 tcaaaaaggc ctcccagccg accagtgcga tcggcggcac aaacccgcag cagttgggca
     7141 gtggcgtagg gatatccggc attacgccca atatgaccca aggcagcatt gtcaacaccg
     7201 gcaatccgct tgacctggca atagaaggag aagggtattt tgttctaagt tccggttccc
     7261 agaatattta cacccgtgct ggggccttct cggtggatgc caactccaat ctggtagatc
     7321 cttccaccgg ctatgtcgtg cagcgcatcg gatctattgg cgagtccgat ggttttcaaa
     7381 ttcccggcaa cagcaacatc aaggtcccct atgatgttgc aatgccggcc agtgccacga
     7441 cgcttgtgcg gttggccggc aatttaagct cggatgcttc tttggacacc ccgcaaacca
     7501 atgttttgaa atccaacatc gctctgcgaa cagtcagcgg tgagctggcc gcttccagca
     7561 ctctattcag tcagctgtca tcggggacat tcgagtcggc agcgattaca gtcagtggct
     7621 atgcccatga tggaaccgca ttgacgcctg cgacaatttc tatcgcagat gacggcagca
     7681 caacaattgg tgattttctt tcagatttga atgcggtact cggagctgac aacgccgtag
     7741 caagcctgca gaacggccga attgtgatta cggatgccca aagcggatac agccgatccg
     7801 acatcaagct ggagttttcc aacagcggta cagcggatat cgaaatgccg gcctattttg
     7861 aaacggcggt agttggcggc agcgaggtga aaaacatcaa cattacagtg tatgattcgc
     7921 agggcggagc gcatgttctt tccggtgctc tggttcgaac caatacagac aatacttggg
     7981 atatgatttt aacctcggtt accggagaca tcagcagtat tgcgatgcag gaccggcgta
     8041 ttaacggcat tcgcttcaat gccgccgatg gagcatttgc cggattggcc gaatcaagcg
     8101 ttccgcagtt taccatcacg tttgctcatg atccctccaa tccccagacg attgaaattg
     8161 ggctgggaac agaaggccgg ctggatggac tgacccagta ttcagggaat tcgaccgctg
     8221 ttgcccggga gcaaaacggc tatccggccg gacgtttgac aaccgtatcg gtgaataacg
     8281 agggcatttt gattggagca ttttccaatg gtatcaaaaa aaatatagcc gccatacagg
     8341 ttgccctgtt tcagaatccg gccggccttg aagctctggg caacggttat tacattccct
     8401 cggccaattc cggagaagcg gtggccacac aggcaatgaa tggcggtgcc ggctctattc
     8461 acggcggagc tttggagaaa tcaaataccg atgtagccag cgagtttgtc aatctgattc
     8521 aggcccagaa cggctatcag gccaatgccc ggacaattaa agtcgctaat gatattctca
     8581 gtgagctgac aaatctaatc cgttaagcag gcaaaagcca gccatgacag agataaacag
     8641 catatggttt attttgaatg aggtgcttat tggtctgact cctttggagc gatgggatgc
     8701 cctgacaaaa tatcacagta gtccggccgc cagtcagcgt cttttaatga tatctgctgc
     8761 tgcagtgggg gcagtgatag ctgcggctgt tatttggaat tggttgaacc gccaatcggc
     8821 tgcaaaaact gacaatcgcc ggtttgatga ttatgcaggc aaaaaacagc ttacggacca
     8881 gcagaagcag ctccttctga gagttgtcag aaaggcggaa ctgaagcaaa aggaatcgat
     8941 ttttaccctt cctgatgtgt ttgaccagac agtagccggg atcattgaac agattcggca
     9001 gacgaaaggg atttccgaag ctttgcggct ggaagagcag ttttcttcgc tgcgtgaaaa
     9061 acttgatttc cggcaaaccg cccctgacag acagatgatt gccgctgcac aagacagcag
     9121 gcagataccg gtgaaaaaaa aactctatat caggcggaat ccccggatga gcggcgaact
     9181 tgaggccaca gtgatcgata attcgccggc gggcattacg attcgatttc cggaaccggc
     9241 ggagattgtt ttcggccagc cgtggacatg ccgctattac acggggggat ttgtggcaga
     9301 atttcaaaca gtgcctgttc aatgccgcgg cactgttgtc gtcttaaggc acagcgataa
     9361 ggttcggctg gtgaaccggc gccggtttgt gcgggtgccg gtgagaaagg cagcttttgt
     9421 tgcggattgc tccttgggca gatcgccttt tgtgccaagg cagatgtcaa ggcgaaaaat
     9481 tacggctctt gatccggttt ccggcttgtc tcagacggat tttgagccgc ctcagtttgt
     9541 gccggcgacg gttaccgagc tgggagggct gggggtgcgg tttgaaacaa gtctgaatgt
     9601 tcaggcaggt gatcggattg tgctggtctt gcggctggaa gaaaccgaaa tgccgatgga
     9661 gtaccagcag ccacccggag gcgaaaaggg tattaccggt attgcccggg ttgttcgtgc
     9721 ccagagaaag gaaagcggat tttctattgc cgctgaatac atttatcttg aggatgagca
     9781 gcttgacgtt ttggtctgtg cagccaacgc ggttctgcat caattaaatc ggggacagca
     9841 ttcggctgct gctgtgctgt cgagccggcg cgtaccggat gctgcagctg tgtagaaata
     9901 caaaagtcgg gaagaaagaa tgtcagaaat atcagcacaa gtcggttcgg ccatacgaag
     9961 cttgacgcag gaatttgacc tgattgctca taatctggcc aatgttagca cgccgggatt
    10021 taagcggcga tacaatgatt tttccaaatc gctgtcagca ttggaacaga cagccggccc
    10081 cgcagaaaat agtcgcaagc cggctaatat agcggtcgat tttacccaag gatcttttat
    10141 cgaaaccggc cggacgctgg atatggcctt gtgcggaaaa gggtttttcg ttatcgaaac
    10201 ccctcaggga cccctgtata cccgcaatgg ggcatttcgc cttaacgaaa acggtcaaat
    10261 tgttgatatg atcggccgca ttgtagctgg tgaatcgggg ccgattaccg tcccgcaagc
    10321 agcaggactg tcgcaaatca atgtggctgc cgatggaacg gttagtgcag caggtgttcc
    10381 gataggaaag tttaagcttg ttgattttct ggacaaagaa aaccggctga cgccggctgg
    10441 tctaaactgc tttgctgcac cgcaaaatat ggattcggaa ccggctgtcg gtctggtggt
    10501 caagcagggc tttcaggaaa gctccaatgt gcagcttacg gaagaactgg tagatatgat
    10561 tatggtcagc cgtctttatg aggccaatat gaagttgatt tctaaaagcg gagagactgc
    10621 caaaaatctg ctcgatgtgg caatgagctg aacacggcat taggccgaaa atcagaaaaa
    10681 ttaggagtac acggatgtta cgagcatttt ccacagcggc aaccggcatg agtgcccagc
    10741 aaatgatggt ggatgttacg gcgaacaatc ttgcaaatat taacaccacc ggctttaagc
    10801 gcagccaaat tgactttcag gatctgcttt acagcaaatt gcgggacttt ggagcggaag
    10861 tatcctcggg aataaaaacg cccggaggaa tggaaatcgg aagcggtgtt cgggccggct
    10921 cgacagtcaa ggtcttttca ggaggggaac ttgtcaacac cagccggccg ctggatatcg
    10981 ctattgccgg cgatggtttt ctgcaggtca gaatgccaga tggctctctg cgctataccc
    11041 gtgatggggc gcttcagatt aacgccgacg gcgagctggt aactgctatg ggatattcga
    11101 tcgagccgtc cgtaagcatt ccgtccaatg cggtttccat tgatatcggc aaggacggca
    11161 gcgtcaatgt gctggacgac agcggggcac agagcgtggt ggggaccttg caattagccc
    11221 gatttgtcaa tccgtcgggt ctgtcaagcg aaggcgacaa cctcttggct gagactgagg
    11281 ccagcggcac ccccattaca ggcacggccg gcgaaaacgg attcggaatg atacaaagcg
    11341 gatttctgga aaaatccaat gtgcagatgg taaccgaact ggttaacctt atcacagccc
    11401 agcgcgctta tgaagtaaac tcccgtgcca tcaaagcagg tgatgaaatg ctcaaaaatg
    11461 cgatccagat tgccggctat taggcaggag attcaatgca atgaaatcca gacgcatggt
    11521 tctattttat ttggcaggca tttttgtgtc agtctgtccc gaggccgccg gaacatcgga
    11581 agaaagactc aaaatatttt tgccgcggga tatcaccatc agcaatactg cccccacact
    11641 cggacaagct gctgtcctgc aaggcagcga aatgctggca gcaaaggctg gcagcgtagc
    11701 attgggatat atggccgagc cgggacaaac tctcgtcatc agtcgcaatg ttgtgctgag
    11761 ccgtctggcc tgcagcggaa ttcctttgtc ttcggtaaca ctgcttggcg ccgaagaagt
    11821 gcgcatccat tgtctggctc gtattgtttc gggaagtcag attgcccaag aagctgctga
    11881 tttcctgaaa gaaaatccgc cggacaaatc tgcttgtcag tttgatattc ttcgaagccc
    11941 tgcggatttg gtgcttgctg atgtctcggg ggatattgaa tttgtgtgcc ggctgatcga
    12001 aaacggctca aaaagccaat gccgggtaga agtagccgct gttcgacaag gacgcacaat
    12061 cggccggcgt gaggtcgtgt tccgatgccg ttatccgtgc cgtcgggttg tgactacccg
    12121 cgctattgcc aagggcgagc ttatcagccc tgaaaatacg gcgatagaaa atggattttc
    12181 cgacagccct gaacagtcag actggtcggt gccctatgga caagctgctg ttcgttcgat
    12241 acctgcccgc accgttctga cagctcaaat gacaggtgct gcaaaacccc ccatcgcagt
    12301 aaagcgcaat caaagtgtag caattcgaat tgacaatccc ggttttaccg caacggccag
    12361 cggaaaagcg cttcaggatg gcgcagcggg agctttgatc aaagtacaga atgtcaattc
    12421 aaaaataatc attctggcca aggttaacga ggatggaacg gtcgaacctg ttttctgaaa
    12481 aggagacaaa atgaatcaga aaataaatca gatttttctt ttcctgctgg ctgcggtcaa
    12541 tgccgttcaa ggcggttcca tctgggccag aaaagacgcc aatgccaaaa atatttatgc
    12601 cgacgatgta gcccgaaaaa ttggagatgt cttgacgatt gttatcaatg aaaccagcaa
    12661 catgaccaat acatccgaac gggagatgtc ccggacaacc tcccgcaata atcagtttga
    12721 cggcaaaatt ggggtcgagc atatcctgac cagcatccca gctgtcaatt tcggcagcgg
    12781 caccacttat accaatacgt ttgaaagtga ggcagacagc aaaaacaagc gggccttttt
    12841 tgacaatatt accgtcgtag tcgtcgatat aatgcccaac gggaatctgg tcgtcaacgg
    12901 tacctgcaag cgcaatattt cagatgacat tcaggagatt gaagttagcg ggattgtaag
    12961 gcccagcgat atttcctaca gcaataccgt agacagcaag cgggtggcca acttcaatat
    13021 catcgccaaa tacaaaggag tagctgcgcc ctacaacaag cccggctggc ttgcacgcat
    13081 cctcgatgta ctatggccgt tctaaccgca cagggagttt aacgccgtat gaaacttcag
    13141 atgataacag ttttgctgat agggacgact ctggttttac cagccggggc cgaacgcatc
    13201 aaggatatcg tcgatattca gggaattcga ggcaatacgc tggaaggcat cggtttggtg
    13261 gtgggtctgg ccaatacggg cgacagtacg ctcccgtcgc gtcagatgct gacaaatatc
    13321 ctccggcggt ccggtctggt gttttcgtcc aacgatctca aaagcggcaa tattgccatt
    13381 gtgtctgtaa cagcagaatt agggccgttt gctcgtgaag gatcccgtct ggatgtcgat
    13441 gtttcgtcta tcggcgatgc taaaaatctg cagggcggga cactgctgat gacggaattg
    13501 tacggcgctg atggacaagt gtatgccgtt gcgcgcggga cagtatttat cggcggctgg
    13561 tcagcggctg gagaaaatgc ctcgattaca aagaatcatc caaccgtcgg gcggattccc
    13621 ggcggggcga tcgtcgagcg ggcagaggta gccagctttg tggaatctgt ttccggaaac
    13681 cgctttatca ccctgaatct gcggaattct gattttacga cggctgagaa tatcagccgg
    13741 gctatcaacg aaaaataccc caattgtgcg gttacggctg atgcaggaac tgtccgtgtg
    13801 gctattccct cttttatagg cactgcggaa atcgggggtt ttattgtgga tataacacag
    13861 cttgaagtgg ctgttgacac gcctgcagtg gttgtgatta atgaacgcac cggtaccatt
    13921 gtggtcggta aaaatgttgg tatttctgct gtcgcaattt cacagggcgg actggttata
    13981 aaagtcaagg agcagcagaa tgtctcccag ccgacggcgc cgtttaccga aggggccacc
    14041 actcaggttg tcaatgaaac actgctgggt gttgaggagt ccgacgggta tctggtaccg
    14101 atgccccgcg tggtaacagt ggaagaatta gccaaggcgc tcaatgccat cggtgccacg
    14161 ccgcgcgata tgattgcaat ttttaatgcc ctgaaaaagg ccggcgctct gcaggcgaac
    14221 ttggaaatta tgtagccgga ggatgcccat gaatccactg cctttaagca acaacacccg
    14281 ctcgccggtg cccctgacag agacagacag tcttcgtcag gcatcccaaa tacaaaaaga
    14341 gcagtttgcc cgggattttg aatcggtttt tatcgaaaaa ctgcttgaag aaatgcaaaa
    14401 aaccatcggc gaatggggtt tggaacagga tagtacagcc ccgcagattc agggattatt
    14461 ccagatgcat ctggcccgcg atgcggccgc aaagggaggc atcggtttat ggaaagagat
    14521 tgttcagtcg ttggagaata tggacagcag gaagggggac attaaagaaa tggataatcg
    14581 tctatgaatt cggatctata tgcgacagaa ctggataact gcataggccg cctgctagct
    14641 gtgctggacg cggatattga atatcttgaa aatacaatat tacggcttgg cgaactacgt
    14701 tgtctggtta tcaagcatcg gtaccagccg ctggaacaga tgctgcagac aatacgaacc
    14761 gaatacatcc gctatcgcaa gaatgaatct cagcggcagg caatttgccg aaaaataggc
    14821 cttctgctcg gttgtccatc cgatgtaatg acactgtcgg atatcgcagc ccatttgccg
    14881 cctgataaac gaacacggct tctcgaacgg agggtaaagc ttcaaacgct gacggatctt
    14941 ctgaggcggg aatatgccgg agtgcaaatg cttttagccg actgcgcccg gttgaaccgg
    15001 atgctgttgg caaacctgct cggaaccggc tgtgctgaat caaaaaccta ccaatctacc
    15061 ggtacagctg aacggcagac agggaccctg tttatggatg cgcgatgttg agtaaggaaa
    15121 ctgacccatg accaattatt cgataggcct cagtggcctg acagctgccc agcgggcgtt
    15181 tgaagccatt ggcaacaaca ttgctaatgc cgctaccgag ggataccatc gtcagcggct
    15241 ggaattatcg ccggcattct cccaaaatcc gggaagcacc gttttcatcg gcggcgtcaa
    15301 tgtggagggc attacacgga taattgatac gctgctggag caggagctgc tgcgtcagcg
    15361 ttccgtgttg gcgtcgctgt cacaggagac atctgctttg gggactatcg aagcggcatt
    15421 tggggaattt gccgatgaag aaagcggctt aaatgcagct atggataatt tttttacatc
    15481 cctgcagaac ctcagcgccc atccttctga gagtatctgg cagaatcaga cggttagtgc
    15541 cgcccagacc ttggcgggcc aatttcggta tctgggacag atgctgacgg atatgaaaag
    15601 ccgtctgaaa tttcaggctg aaacctgtgc cgatactatc aatttgctga cctcccaagt
    15661 ggcagaactg aacagccaaa tcgaacgtat taaaatggtc ggcggagatg ccaacagcat
    15721 cagcgatcag cgcgaccggt atattgccag attggccgag ctgattgctg ttgagaccat
    15781 cgaacgcgaa aatggcgtga tagatgtcag ttcaggtggc ctgcctctgg ttaccggcgc
    15841 cagcgccaat cgcatcgaag ccggttatgc cgacacaggc cgattgggta tttccattct
    15901 gggagcttcc acctatacga ccgccatcga aggcggaacg gtaggagggc tgctgaccct
    15961 tcataacaga attgtcgccg gtattcaaga agatttgaat tgtttggcta actccattat
    16021 tcagcaaatc aatcaatacc atgcagcagg aatcggctcg gccggctcct ttaccgagct
    16081 gagcgggatt tccctgaaaa gtgatcaatt atccgatatc ggctgtgtta ccgacggcac
    16141 attttatatt cggctgacca atacagtctc cggtcaggtc agccggcatg ccataacagt
    16201 caatgcggcc tccggcacat tgaatgatgt agcagcatcc atctcattga tccccggttt
    16261 aagggcatcg gtaaactcac tcaaccagct ttggattcag gcggattcca attatacatt
    16321 tgattttctg cccggcgcct tgccggaacc aaccatggtt tcttttgccg atgtttctgc
    16381 cccctctgtt gctgtggcag gcatttatac aggtacagaa aacgatacgc tgcggtttac
    16441 agtcaggggg gacggcagcg tcggcagcgg cagtcttcag cttgaggttc gcagccggga
    16501 tggcagcggc gacctgctga ctgtgctgaa tataggggcc ggctatgcag ccggaaaacc
    16561 cctcgaactg ggcaatggat tgaccgttgc attgtcggtc ggcagtctgt ccgaaagcag
    16621 cggcgacagt ttcagcatag atgttttggc cgacagcgac tcttccggtt ttcttgccgc
    16681 tgccggatta aacgtctttt ttaccggcac atcagccgcc gacatggccg tctccagacg
    16741 gatcgaagca aatccccgtc agatagccgt atcgctggga gccgacggcc atgataatat
    16801 caatagtctg cgtctggcgg ctgtcagaga taaaaccttc agccgtctgg attcactgac
    16861 gtgcagcgat ttctaccgaa agatggcagc cggaatcgga gaggaactgt ctgtgcgtca
    16921 aacgcagcag acaaccgccg aaacggtgct gctggatctg acaaaccgcc aaagcgaaat
    16981 cagcggcgtg gatatcaatg agcaggcggc tgagctgttg atctttgagc aaatgtttca
    17041 ggcaatggcg cagtatctca atacaattaa aacatccctg tccaatctga tggacatcct
    17101 gtaaggaatc acagctatga gcggattaaa tgcactgtat aacaatgcag cttttgcgct
    17161 gcgtctgcat gcagcggcga tggctgtgtt gcaggagcag gcctcaaccg gctcacgcat
    17221 taatcgcccc tcggacgatc cagcagcggc cagccgcctg ctgggcttat cgtcgcagca
    17281 aaagtacctg aaagcctata tctccagcat cgattctgcc gtcagcactc atgagatggc
    17341 ttccaccgtg ctgcagcaaa tggcctctgc gctgaccgaa tcgatagtcc gtctgaccca
    17401 gattgtcagc ggcacctatg gagaggggca ggaaggccaa aatgcccgag accgcactgc
    17461 cgcggagata aacgatgtcc ttgaacagat ggttgccttg gcgaacacca agtatgtaga
    17521 ccagtatatt ttcggcggga ataatactgc cgatatccct tacgcagtac agcgcagcgg
    17581 gggtgaaata acggccgtgg cgtattgcgg ttcttcggaa aatcgctcca tagaagcggc
    17641 tcccggcgtc ttagcctcta tatcccttgc cggcgaaaat ctcttttctt ccgatagtcc
    17701 ccagaccccg gttttttacg gcagcaccgg agccaaagcc ggaaccggca cctccactat
    17761 tcggggtcaa gtatggctga cagtgacttt tgacggcagc agttattgtc tgtccatcga
    17821 cgacggcgca acccagatac aagtcccgac ggaaggggat ttaagcaata tcgctgtatc
    17881 tgacgctgcc ggccgcgtcc tgtatgtcga tgcttcaaat ttaacctctg ccggtgttga
    17941 aaaggttaca gtgcccggca cggaagatgt ttttactgcc ttgatcaact tacgcgattt
    18001 gctgcgcaat tcccgcggtt ttacagatgc aatgctggcc gacctcatta aacactcttc
    18061 tgattcgctg gaatcggtca aaaatagtct tgttgaaaaa gaaaccctga taggtgcccg
    18121 tgttaatttt ctggcgtcgc tgaaggataa tctggaaaat atacagttta accaacagca
    18181 ggaggcagcg atgattcagg aagccgatat tgcccagatt gctattgacc tttcacgccg
    18241 ggaaaatctg taccagatgt cgttgcttgt ttcgggcaaa ctgatgtcaa tgtcgctgct
    18301 ggattttcta aaatgaatga acaattgtcc ggacagctgt attggatttg aacaggcagc
    18361 gtttttcctg tgaaaaaaat atcctgctat ggtttgctct tcacataggc acacattttg
    18421 cacatctgca atgcagactg ggtaaaattc tgggaaaata gtttagatat ggagtgaata
    18481 gtaccgtgca taacatccct gcagacaaca aacatgatgt aactgacggt gcaatattgc
    18541 tggaacgagc cgactatttc ctgtctgtcg gcagcttgga gcaagcccga tattattatg
    18601 aacagctcgc tgtgttatgc cctgatgaac cggcctccta taccggatta ggtatcgtag
    18661 ctctgcgaaa ccatcagctc gacgatgcac agctggcttt tagggtggct tgccggctgg
    18721 atccgatgca tgctagggcc tatgaaggat tagccatgac ggctcagcag aagggtgatt
    18781 atgagacagc attcgatatg taccttaaat gtcttgaatt agatccggat aatctgtccg
    18841 cacttctggg attgtttcag acatcttccc gtatgggttc gtttggacgg attatttatt
    18901 atcttgaaat gtatttgaaa aagcatccgg acgatgtttc tgtgctgttt tcgctcgcaa
    18961 cgctgtatat taaagaagac cgtttcgaca cagctcaaaa attgctgcgg gatattcttt
    19021 gtttggaacc ggataacaag gatgctaaac atcttttaga ggaagtccag cagcatttag
    19081 cccgggtgat gtcctgatga cgcgtcaaat cacaaaacct ctggaagatc tgtttgacca
    19141 attggatgct gtgttgaaca ggatggaata tctgattcga aaaagtgatt ttgacggcgt
    19201 ggagatgctc tgtcagaagg cggatcttat tattgcacgg ctggacgata tgcggctgtc
    19261 cggttatccg gagctgaaaa ctcgctgcaa acaggttctc gggaggtatg aaagacttgt
    19321 gttgatgctt cggagcgctc aggcagcgat ataccagcag atgcataaat tggagaatgg
    19381 gaaaaagata attcaggcct ataatgggaa ataatataca tatgtttcca gatttggaat
    19441 tacaaaatga cagagattct tcataaagtt tttacagaac aaatcgacaa tataaatagg
    19501 tttgaatttc taaccgcaaa gatattatag atattacaag tccgataagg aacctttatg
    19561 ggacaaataa ctcttacggg gctttcaacc ggcgttgata cctctgctat tataaagcaa
    19621 ttgatggtga ttgaaagccg ccggcttgct acctataaag tcagcaaatc aaattttgag
    19681 aagcagacaa cggcactgaa ggacctgcgg tcaaaaattg ctgcagtcaa gtccgcagca
    19741 tccgaactgg caaatgtccg gaaaatgcag cttttttcag ccagttcgag caataaagac
    19801 attgttagtg ttaatgcatc ttctgaagcc aatcccggct cgcataccat acaaatcaat
    19861 cagatggctg ctgctgacgc atggatacag gaaacatcgg tatttaatta caaaaccgat
    19921 tatgtcggag ggggcagttt tatctatacg tataacggac ggcagcgcat tattacagcg
    19981 gttgccggtg aaacaacgct ggaagatttg gtcaatttga tcaacaacga cgagaacaat
    20041 cccggtgtta ctgccagtct gctgtatcag tcaggcaagt atcatctgat gctaactggc
    20101 cacgaaatgg ggcaggattt tcagatttcc atcgattcaa gcactcggga ggtatggaag
    20161 cccgactcca atcagccgca cagcaccttt accagcagcc agctcaatgc ctctttgtcg
    20221 acaaagatta taaatcttga tcagttcggc ggcaccttgg gaacggccga taaaatcatc
    20281 atttccggaa aaaatcatgc cggttctaat ctgccggata cggaaatttc tgttaccgaa
    20341 aatacaacac tggggcatct aatagatgcg attaacaggc attttgacgg aacggcgaca
    20401 gcccggcttg aaaatggtca gatatggctt acagataata cctgcggttc cagcggtctg
    20461 gaaatcagtt taagctattc cggggatgcc gtgcttcatt tgccgtctat gacggtttct
    20521 gccgagggag gaagagattc agaaagtttg gctttgctcg gctctgcgtc ttttatccag
    20581 acgcaaaatg ccagtgatgc acagattaaa attgacggtt ttccgttcag tacggccaat
    20641 gaagttcaaa cgctttctat aaccggcggt atccctgctg ccggcacctt ccggttgact
    20701 ctcaatggac aaaccacaga tcctattgcc tataacgctt ctgctgccga tgttcaggct
    20761 gccttgctga atttatccgg tgtagaggaa ggggatatcg tatgcacagg aagcgctctt
    20821 cctgccggcc ccattgcgat cgcctttggc ggcagactgg ccggaatgga tgtttctcag
    20881 attaccgtca gcgatgacgg cagtttagat gccggtgtcg tgtcaatcgt tgaaacggtc
    20941 aagggcagcg acggctggat tcaccgaagc agcaatcata ttacggatgt tcttgccgga
    21001 ataaacctga cccttaacga tgtaacgccg gcagataatc ctgtcaagat aaccgttacc
    21061 cggaacaccg caacccttac caagaaaatc cagagctttg ttgcggctta taacgatctg
    21121 atggatgaac tggaatcgaa aacagaatac aatgcggaaa caaagacgat gggaatttta
    21181 agccgagatg ttgccgcagc gtttctgaaa actcaggcgt ccaccccgtt cacagccatt
    21241 gccagaggat tcctcgaatc agctgatgcc tttgtgcagg ctgacggcat cggcattacg
    21301 ctggatggaa atggccggat ggaattggat gccgaaaaat ttgaaaaggc actggcggca
    21361 aattatcagg cggtcctcga actgcttgga gcggccaaga agggcgccag cagcaatgcc
    21421 gccgtgcagg tttacaatac aagcgagaaa tacactgccc ccggcattta ccatgtcaaa
    21481 attgaggttg atgaaagcgg cagcattgtt tcagccaaga tcaaactgtc aacggaatcg
    21541 gaatatcggg atgcagtctc atggagcggc ggcatcataa cgtttgacag cagttttgac
    21601 ggcactggca atcccctctg gccggaacac agtctgcaat tatcggtgga tgctgttgcc
    21661 ggaacctatg ggacggatgc caatccggta gtggtccgca tcaaacaagg catcttcggc
    21721 aatctcaatg atatgctgac ccagctgacg aaaacaaacg gccagcttga tgtcagcgcc
    21781 gaggcacttg accagagaat atcccgtatg accgccaaga tagagaagga ggaagcacgg
    21841 ctgaagaggg ttgaacagcg gcttacggaa aaatacagcc ggctggaaaa aatactgaca
    21901 gcgatgcagc agcagatgaa tgctgtcagc gctatgtaca atttttaagc ggctctcagc
    21961 ggcggctgcg gcgcagccga taaaccatcg ccagcacttc ggcaacggcg gtatataagc
    22021 cgctgggtac cggctgtcct tcttttacag ttgcaaagat ggttcgggcc agttcgggtt
    22081 ttcgaattat cggcacaccg taagcacggg cgatctctct gattttttca gcgatgtggt
    22141 cagccccttt ggctgtcagc accggggcac tcatccggcg cggatcatat ttcaaggcca
    22201 ctgccacatg ggtagggttg acgataatga cgtcagcctt ggggacttcc tgaagcatgc
    22261 gcttgcgtgc aatggcaatc tgtatccgcc ggatgcggag tttcagttcg ggagaacctt
    22321 ctgtatcctt ttgctcctgt ttgacctctt gtttggtcat tttcatatcc atcatatatt
    22381 tccatttctg gaacactact tccgccgctg caatcaccag aactgctgca cacacacgaa
    22441 gcagcatccc aaacagcagt tttgaaaatt ccgccgcaag ctgtgccgac caggcccacc
    22501 gcagggaggc aagggaatcg aaacggtttt ccaagtaacg ccataccaca atggaaataa
    22561 tcagaaactc ggcggcagat atcgccagtt taatcagtga ccgaagcgta aacagctttt
    22621 cgaacccttt aacggggtcc aattggtcca gccttggttt aaggggttgc agcgagaaat
    22681 tccacccgct gacggtgatg ctggacaata cagctccggc cagaagagct gccagcaaag
    22741 gagctgtcac gaaaagagcg ctgcttaatt ttgcattcaa gtactgaaca aaagttgccg
    22801 gatcagcgaa tacccccgat tcgccgtaca gtccctcccg gagctgcgtc gtaaaccaat
    22861 tcagcagaaa gggtgtcaag gccaatccgg caagaacaac agctgctaat gagactgctg
    22921 ccggaaattc ctgactctgg gcaacctgcc cttcctgacg ggctttttcc agccgcttgg
    22981 gtgtcggctg ttcagttttt tcggaagcag gtctgtcttc cataagcggc agccaatgag
    23041 gttctgttag acttttatct tcttacaagg gcaggagttt gcccatccaa tcagtcaatt
    23101 caccaataaa cgaatgaata aacggaaaca tcattcctgc tatcagcagt cccagcccgg
    23161 tccgcagcgg catgctgata aataggatat ccatctcagg aatcatccgg gcaaagacag
    23221 ccaatactcc cagcagcagc ataaacgccg ccagcatcgg tgccgccaga cgcaagcctg
    23281 ctagcagcat tgtggagccg gcttggacaa tgccttcagt caggagcgca taagagggaa
    23341 tctgtcctac tggaaaatgc tcgtagcttc tggaaataac cagcagcagc agatgatgac
    23401 cgttggctgc cagaaataaa agaataaaga tcatttccat caaaatgctc aggggttctg
    23461 ctgtttctcc agatagggga tcaagcattt cagccatagc caaccccatt tcccgttcga
    23521 tcatttgccc gcccatcttc accgcagaaa acagcagcac agcaatcagt cccagtccca
    23581 gtccgtacag cgcttcattg ataataagaa cagtcatccc cagaaaagaa agtcctgctg
    23641 tcggaatgac agagggaatg gctatcgaca aagacacaga taccagaatt gcagcggctg
    23701 cccgaatcct tacggggact gtttggctgc cgaaaatcgg tacgatggac agaaaaaccg
    23761 atgtgcgtat caaaacggca ccgaatccga gcagtttaag caaaataaag tccatcctct
    23821 cagcgtccga tctgctggat gtagccgaag atatcgctgg taaagcgaat catccgctcc
    23881 aaagcccaat gaccgctcag aagaatcacg attcccacgg caatgagttt tggaacaacc
    23941 gttaaggaca tgtcccgtat cgatgtcacg gcctgaaaca gggtaatcac cacaccgaca
    24001 gcgatgcatg tgatcagcac aggtgcggat acgaacagcg ctgtttccag cgtttgtttt
    24061 cccagaaaaa taaccgcgtt ttcatccata aacggccgat gctctttctt aatgaaatcc
    24121 aagtcccaaa cttttggcca gcagtcccca gccatctacc aagatgaata aaatcagctt
    24181 aaacggcagc gaaatcatga ctggcggcag catcatcatc cccgcgctga gcagcacgct
    24241 ggaaatcacc aaatccacaa ccagaaacgg gatgaacagc aaacagccga tttcaaatcc
    24301 tgtccgaaac tcgctgatga taaatgccgg aataacaata tgcagctcga gcatagcggg
    24361 gctggcaggg gcttcgatgc cggccatctg tacgaaaagt gctaaatccg cctgacggca
    24421 ctgccgcagc ataaaggctt tcattgcctc tttggcttgc tcgcttgctg ctgcaaagtc
    24481 aatctggtct ttcaaatacg gctggatgct cgtttcgtta atgatggaga aggttggtgc
    24541 cattgtgaac agcgttaaaa aaagtgccaa tcccatgatg gcaatgtttg ggggaatagt
    24601 ctgagtcccc aaggcccttc ggacgaaaga caatacaatg acaattcggg taaatgatgt
    24661 cgtcatcgcc atcaaactgg gaaggactgc cagaccggta aagataatca ccagtttgac
    24721 cggtgttgac cattccttac tttccgcacc ggcggatgtt gccttatcga cggcattcag
    24781 caggtcgcct atcggtggaa ctgctgccgg ccggcgtcca tcgttagaag tcggattctc
    24841 ctgcgcccag catacggcag gggtgttaat cgccaacaca atgagcaata agaatccaat
    24901 ttgtttataa tacattgggt gtatccatta gttttgtttg gacagccggc gagacatttg
    24961 acgggacctt gtcgaagctg tccgacagcc ggcagatttg atcattggta cagccgatca
    25021 ggatttgacg gccattgatt tccagcagat gcagcgtccg gcgggaaccc agataaacag
    25081 tctcgacaac ctttatttgt ttgccatgaa caggagtcag ccgcggcatt acttttttgg
    25141 atgccatgag tgccgctatg cccaatacaa ccacaaccaa tgttcctatc agcatccggt
    25201 gcagggcgct gctgagattt tcatccggat tgcccgattt ttgtgcttgt tgggtccata
    25261 attcaatatt agaagaattc tgtttttcga cagccgatac cgtgttatta ctcatcacaa
    25321 ctgtacagaa aagacacaac attatcttta aaatgacgct gctttcgtat cttaacaaga
    25381 taacacccct gttttacgag taatggtcaa cattttctgc acagttgata gtgtgcaaat
    25441 aatatgccag aaaatttccg aagcagctgt cgaaagaata atatggtttt taatctatat
    25501 ctttctgttc agaggtgtcc aattttggaa gtgcacgcat ccggttttgc tgtcattgga
    25561 ttttgtcaac ccggcgaacg gccaagagaa tatgtgtctg ctcggaatcg catttttcct
    25621 gagaaagcca atgtccccct gcaccggctc cccacgcata taccggacat tgaatacccg
    25681 attctcgcag aaagtccgcc acattctgcg ctcggagaga cgaaataatc cattggtttt
    25741 tttccgttga ttcgtcacca gccagcccaa ggacgtatat ttcaattgtt tcggcggctt
    25801 tctgctgaag atgcattgca aattgggaaa gatatttctg atcggcgtct tttagtgccg
    25861 cttgtccgcg gggaaaaaca atacttgtaa cggtataatc agacggccgg atttcgattt
    25921 cggtgcgtac agcatgcatg gatttaatga ccttttgaaa cagccggcga agatcctcct
    25981 ctttggaatc aattattcgc tgcggcggtt tttcatccgg agcaggagtc tgatatttga
    26041 tctttaattc tcccagctcc ggcaccagct gcttgccggt cagcattccg agaccataac
    26101 aatcgatatg ggcgataaat gcgtttcggc ttcggttaaa aagttccgga tcccgtacct
    26161 gtgccaaact caaaagcatt acaaaaaatg tcagcagcag cgtgaccata tccgaaaaag
    26221 taacaatata agccggcaca ttggggcttc cggattcctg ctttttgcgg gatttcagca
    26281 tctcagcgtc ctcccggaag catcacaatc tccagcatcc gctccgaaac gggggagggt
    26341 gtttctgggt cagacggcac ggcataatcc attgtgccgg ccgcagagag acagaaccgt
    26401 cggggatcaa gcccgtgttc ttttgtcaaa tagtctatca ccgtcccggc acgctgaatc
    26461 ccgtaattgg gtgtcaactc gtcagaagag gatccgcttt cgctgaccat aatcaggtga
    26521 ttggggaact ctttgagaaa caatgcaaga ttataaagag cctgtttgcc ttgaactgac
    26581 agtaccatcc ccttgcccca gaatgcttcg ctggagggca ttacaaattt tttatatttt
    26641 aaataatcgg ccggagacgt tgtatttcga gagcgatttt tttccttttc ttccaaggtc
    26701 ttcttttcgc tgccccagtc caattcataa ttcgggacct gctctcggat aaaaacgaca
    26761 gaatctttct ccgtctggct ctgctgaccg aatgaaaact gctgggccat cgaagatgtc
    26821 attttcagat aatcatcttg gttgtcaaaa gatgaaaagc tcagcagaag cacaaaaaac
    26881 gtcagcagca gcgtcatgca gtcgctgaat gtaaccatcc attccggtgc accggcttcc
    26941 ggtgccgcat cggcattttg tttgtcccga gccattaaat gacggccttg atatccttac
    27001 ggctgctgtg cgacataaac gcctgcatgc gttctctgac caaggtcgga ttttctccct
    27061 gcgcaatggc acacagccct tcggtaatca tttctttcaa aagggtttcc gttttcgagt
    27121 agattcccag tttgcctgca agcggtataa aaatcagatt cgaggcaaat gccccgtaaa
    27181 aggtggttac cagtgccgtt gccatcccga cacccaagcc gctcggatcg gacaagttgg
    27241 acagcatttg aatcaggcca atcagggttc caatcattcc aaacgccggt gccgaagctc
    27301 ccataaattc aagaattttt ttgccgacag agtgacgttc ttggaggtat tcaatctcca
    27361 atgtcagata gtcccgtatc gtttcttcgt tctggccatc cacgagcatc tgcagaccct
    27421 tggcgaagaa atgatcatct agtttgtgaa tttccggctc cagcgccagc gccccatccc
    27481 tgcggttgat ggcggcgtaa ttgaccattt tctgaatgat gtcggcttgt gccggcagct
    27541 tggaaacaat cgtttttttg ataatcgaaa aaatactcaa aaattgagac agtgaaaaat
    27601 gaatcagcgt agcacaaagc attcctccaa tggtaataat cagcgacggt acattaaaaa
    27661 acatttttgc cccggcgccg actgctatcg cgccgaggat gaatacaagc cccagcagaa
    27721 ctccgataat cgttgcgatg tccatttgcc tactccgctg acagccgttt taaacgctgg
    27781 caaatataaa ccgacacggc gggttctgct tcggcaatcg ctgccaacgt cttggctttc
    27841 gtgcgttccg ccatataata aagaatctta acagcatcat cggcaccggc cgctgcactg
    27901 ggatttttca ccattgccat taaaatgcgg gctgcgcttt cagcgtccat tttgtcataa
    27961 gcggccgcaa tcgaagtaag attttccttc tcattctgct cgattacaag ccggctttgc
    28021 atcaggcggt cctgctgctc cttcagggca gcggcagcag cggccagttc aacacgcagc
    28081 tgctccattt cttcaatgtc tttcttgagg gattcctgtg cggcctgcag acgcttctcc
    28141 tgagtctcca gattgcggag tttggaattg tattcgcgta ttttttcccg tatttcatag
    28201 gtaagcgtct gaagctgctg gtctgtcatc tttattcggg acggatcgcc cgccagcgcc
    28261 tcttgaataa tcccgacggc agcctcagac gacatcgtac tggtcttttc ttgttcgcct
    28321 gtaccggcgg tttgggcgcc ggcagctggt agatgcgctg caggtttggt tatccagccg
    28381 acggcaaaca tactcccgaa gccgaccaat ccaatgcctg ctgtgaatgc agctaatttc
    28441 agtttcattc tgtcacctct tcaatgattt gtatgccatg ttttactctg gggcagcttg
    28501 ctgcaactgc atcagacgcg caaaacgaaa agcggtttct tcgtcctgct gtttttgttc
    28561 cgatatttcc tgcttttgtg caaattcctc ttcggcccgg gtcctcagcc gctggagggc
    28621 atccgtaaac tgttccacac gggccagttc agcctctttt tttttctgct gctctgacag
    28681 ctgtttaatt tcgccttcca gccggcggat agccgagtca ttggcttctg aatatctcag
    28741 gaaaaacgcc tgctgttcaa gccgcctgcg gggcggatcg gactgcaatt tctcaatcaa
    28801 attctccagc atctttttct gcagcatcag ttcacagcgc ttgcggccca acaagacggt
    28861 caaggcagac agttccgccc gcaggagctg catctgcttc tgccggatat ccaatacacg
    28921 ctgcagccgc catctgaatg ttgacattat ttaacagccg ttgaaacact ctgtctgttt
    28981 ttatcgctgt cgctgctcag taattcatac catctgtcgg taatttccgt catctgtcga
    29041 atggtttgct caaaggaagt ccggtcgccg acccgctgca ccagaaaatc ccgaactcgg
    29101 tcaatcaggg caaccgctcc gtcaatccgc cggttgctgc ctgcggcgaa aataccgatg
    29161 ttaattaaat cttccgcatc cagatatgtc gcataaagtt cctttaatct ccgggcagcc
    29221 agcttgtgct gttcagaggc caccatcggc atcagacggc tgacgcttgc cagaatatca
    29281 accgcaggat aatggccccg gtccgccagc ttgcgcgaca agacaatatg accatccaga
    29341 agactccgtg cactgtctgc aaccggatcg ttcatgtcgt cattttccgc cagtaccgtc
    29401 aaaatccccg tgatgcttcc gcggtccgaa cagcccagcc gctccaccaa tcgcggcatc
    29461 agagaaaaaa cactcgggca ataccctttg gctgccggcg gttcgccggc ggcaagacca
    29521 atctgccgct gggcctgtgc aaatcgggta aatgaatcca tcataaataa aacatgcagt
    29581 cctttgtccc gaaagtattc tgctattgcc acagcagtga atgctgcttt gacacgctga
    29641 atcgggggcg aatcggatgc tgatacaaca acgacgctgc gggataagcc cttcgccccg
    29701 agatttgttt cgagaaattc tctgacctcg cgtccccgct caccgatgag agccacgaca
    29761 ttgacatcgg cgtcgctgga gttggcaatt tctcccaaaa gaacactttt gccgacaccg
    29821 cttccggcaa aaattcccac acgctggccc cgcccgcagg tcagcagacc gtcgatcgat
    29881 cggattccca aagccagagg atgggtaatt ttttctcgct gaagtgccgg cgggctttcc
    29941 gaatcaagag ctttttgctc gctgccgacc agcggcccgc gtccgtctat cggttcgccc
    30001 agaccgttga gaaccctgcc caaaatatct tcgcttacat ttatatagcg cggcgtacag
    30061 cgggctgtaa cgcggtcccc gggcgagatg ccttcgatat gttcaagcgg cagcaaaatc
    30121 agccgctctt gctgaaagcc cacaacctcc gccggaatgc gcttgccgct gcgaagatga
    30181 atatagcaaa gctcccccag accgaccttc gggccgctgg attcaatcgt caatcctgcc
    30241 acccgcacga caattccatg acagctgccc agattgatgt tctgcagcac cgatagcatt
    30301 ttattgatat caaggcgcgg cctgtctgtc ctgaatcttg tattcgatct tggctgaggc
    30361 ggcggattgt ctatagtctt gtcgagcatc gcttaatgtg tttttgcaag tgcttttcca
    30421 acttgttcca gctgttcatt gatcagatag ctgacgatcc cgcggggact ttctaccaca
    30481 cattcggcac ggccgaggga ggcatctgcc gtcagttgaa taccttccgc cgcagacgga
    30541 tgttcctgct gaagggattg gagatccgac aggtcctgcg gattcagccg cactgtgatg
    30601 ctgccgcact tgggtacagc atggagtgcc tctcggaccc gcgcctcaat gtcataatct
    30661 ccttgactga ttttacacat cagaatcttg cgggcgattt caacagacag ccgggcgata
    30721 gcttcctgat gaccggccag aagctcgtcg caaaaccggt gcatctgctc ggcagcttcc
    30781 tgaaatatca ggcaggcatt ctgataaagc tgcttctgtc tgtccagtga tttccggcag
    30841 gcatcacagg ccggcccgct cgggccgccg gcggatattt ccgtgcttgc agcaccttgg
    30901 ctgtccgcca gcttcacagc ggcaattggt ttgtcaacag aaatcaccag tgcagaaaac
    30961 atggctctat tcttcgttaa aattcaattc gcctttttca ttcatctgcc gcagcagggc
    31021 tgtaatatcc tcccgcgcct gctggatatc tgcttttcgg ggagacgcca aaagagcggt
    31081 ttcctcatcc agcatcgccg ctgcacgctc ggacatattg gctttgattt tccgggcgac
    31141 gccctcatcg gcgctgacca gcgcaagagc cagctttttc aaatcggcct gccgcagcgc
    31201 ctcctgcagc gatcggtccg caatatcggg aatatcctcc cacagcagca tctgttctga
    31261 aatcagccga cctgcttccg catctttctg tgcaatgctg ctgatcagac cgtctcgaat
    31321 ttctttgctg agattgcgga gcatcaaagc gactttacgg ggcgcctttt ccaagccggc
    31381 ggtatcaccc tgtgcggatt caagccggcg gcataccatt tgagcaatcc gcatctttgt
    31441 gtctgccaga acaggttccg ccgcagccat tcgcgtcacc accttcagcc gaagcggctc
    31501 atccagcaag gccagcacct cggagctttt acgggcaggc aattctgcca gaacgaccga
    31561 aacggtttga ggatgttcgc cggcaagaat ggatgccagc gttttggaat cggcatggcg
    31621 aatggcaata aagggatcgc gtttttgaag caaatcccgt atctgggcct gtatgcgttc
    31681 tgctttttcc ggccctacag tcatcttcag catcgttgcc agaaaattct gaacatccag
    31741 tccgcggctg ttttccagcg atagataaaa ttgacgggca acctccgtgg tttgactgat
    31801 gctgctgaat ccggatgcat ccaaataggc cacctccacg gccagctcct gcactgtctg
    31861 cgggtcaaca cccttgagca gttcagccgc cgtagcggca tccagcccca tcagcagcag
    31921 tgccgctttt tgttttccgc tcaagaccac cggctagcgc cccttttctt gaagccagct
    31981 tgaaaaaagc tgcttgacct gttcaggatt gttccgcaga ctgacggcaa tctgtctgcg
    32041 aagcatcatc gattcggaca tatccggcga agctgccggc aggccggcga aagaaccgct
    32101 ggaagcggcc cccagcggac tgcttggctg ggcaaaggcc tttttgccgg ctccgccgaa
    32161 aatcttcaga gctatcagcg cgcaaatggc cataatcccc agagagcttt gccgggcaat
    32221 ttggatgtaa tccagccgtc ttgtttcttc ctccgccggt acatcggccg gacgattgaa
    32281 cttgacatta accaccttca gcccatctgc cgccagcttg ggtccgacag ctttacgaat
    32341 gatatcctct atttccgaga cgggcataat gggctcttct gaagcagccg gtgccgcttc
    32401 gtcgccgccg gctgccttgc tctcttgcgt caaatcaacg aaggctgcaa ctgtcagcga
    32461 tttaatttct ccggccagag caacagtctg ctgcaccgtc ttgggaacag cgtattccgt
    32521 aataatagtc tcgtttttct tggtattgcc agccggcgat tcgccttccc cggccgcctt
    32581 ggcaccttct atttgggatt cgttggtaat ctcttctttg gtggcgaccc gcttgccttc
    32641 gtcgtaggtt tctttggcag tgttgatgct ggtcatatcc acctcggcac tgacttttac
    32701 cgatgcccgg ccgggcccga gaaccgccgt cagcatatcc tgcacctttg tctccagatt
    32761 gtgttcgatg cgttcccgat agtctgctgc tgttcctgca ccgcccggca gcgcatcgtc
    32821 agtttgacgg gacagcaggc gtccttgact gtcaactatc gtaacgtttt ccgctttcag
    32881 cccttccaca ctgccggtta caatatgaca gatagccgcg acattttgag cactcagcat
    32941 gcagccggga cgaagccgca gaatgacgga tgcactggtc tgtgttttcg ataagctgaa
    33001 gtagttgttt tcaggacaga caatatgcac ccgcgcatgc gtaacgccgt caatcatctg
    33061 aatgctcttg gccaattcct cctgaagagc acgcttgaga ttgacgtttt gaacaaacgg
    33121 gctgacgccg accttttcat catcaaaaag actgtatcct ttctggccgg tgcccggcag
    33181 accttctttg gccatatcca gccgcagctg gctgacatgc tgctgaggaa cgtaaatggt
    33241 agtgccggcg ctgctgagtt tatagacaat gcctttctcg ctgattttat cggtgatttt
    33301 tgcggcttct tcaggatcaa gaccggaata caagactctc atctcgggca tccgtgccca
    33361 ataagcaaat aatccgctga ccagcagcac tgctaaaacc attgacacca gcagcgcccg
    33421 ctgaacaatg ccgattgcct gccacaaagc cactgttttc tggaaaaaac tcatgacagt
    33481 ccttcaccgc tgaatggacg tccctgcccg gagcagccgg ccgtcgttta cagcggcata
    33541 ttcatcgttt ccttatatgc ctctacaagc ttgttgcgaa ctcccaccag cagcttaaaa
    33601 ctcatatctg cacgggccac atcagaaacc acagccgtaa tgtcttcatt tcggccggca
    33661 agcaaatcac ggatggacac atcggatgcc cgtacaagat catcaacttt gttgagttcg
    33721 gcctgcgccg cttttacggc atcctgaaag accgacccgg aatctgcagg tttcgtgcct
    33781 gctgggcgga tatttccagc tgccgaattg ggaaaggaag acggaataga gggattaaac
    33841 tggatcattc gacaaacaat cctatctaag cagttcgatt gttgtttcta ccatttgctg
    33901 gtaccgtttc aatacagccg cgttggcctg ataggtgcgg acggcggtgt tcagattcat
    33961 catctctgtc ggcacatcca cgttcggcat ggctacatag ccctgctcgt ccgcatcggg
    34021 atgccccggc cgcaggatcc gcggaaaatc gctcatatcc tcagctattt tttcaatctt
    34081 gactccggcg atgcccttgc cgtcggtttt cagtattgct tctacccggc ggtagggctg
    34141 tccctgtcca ttatcggtcg actgggcatt ggccacattg gatgaaatca gctccatatg
    34201 tcggccttgc gcccgcaaac cggataccgc tatgtcgatt gtccccaaac catttacatt
    34261 catctgtatg cctctttgtc tgctgattca ggaaacggca gccagccgtt ctgattattt
    34321 aatgttcatt gccaaatcaa gctgggcgta ctttttctgg aggagccgaa tataggtttt
    34381 atggcgaagc gtgtttttga ccatctggcc gacttctcct tccaaactga catcgttgcc
    34441 gtttgactgg accggcgttg tttccggctg gtagatctgc ggttcaaccg cactggcatc
    34501 agcggcatcc gatgcagata aagctttggc aagaagctcc tcgaacttta catcgatccg
    34561 ccggtacccc ggcgtttcga ggttggccac attgccggct atggccttct gccgcaaggt
    34621 ctctgcccgc aggccggcgt tgagcaattc tgtaatggtg tctattttcg tcatattaaa
    34681 aagattcttg tttcgaaacc attcgtatcc gcaacaagca tctgcaagaa ttgtgccaaa
    34741 aaggccgctc gtattttttc ttgcttctgt tagatgtatt tttgcagcaa ataagatcgg
    34801 gtgtatggaa cttataaagc ggcttccttc ggctgtgtcc ggtttttaga cacctccctg
    34861 ttcttgaccg aagttcagtt tagacaatac cagcatcaat ttcttctggg acagaattta
    34921 ttatcttgct gcaacggaat tttctgccgg caataaagtc cttatttgcg gttttcctca
    34981 ttttttgtgg ggctataggg ttttatcttg ctttctaaat ggtttctata tttatctgca
    35041 ttaaggactt atgtgttctt ggctggtttc gtataagact ggcacgccaa ttgcaccttt
    35101 tttactgtta tgaataccgc caataccgcc atgatgacag tatgtaatat tttaaaccag
    35161 caactcggtg cttcgtcggt tgataagacc aaaacaccga atatgggtga tgctccatat
    35221 atgcccgatg cccacagtca aaacttagat aggtcctata ataatgaatc gggcggaagg
    35281 attgcgattc caaaccaaga taataagtct gctttcaatc acaagcttgc tgaaaagata
    35341 aatgcgcaaa caggcggttg tgtttctctt ggctgtaagc aggtttcaga aaaaaaagag
    35401 gagaaaacag cgtctttggc ggatattccc atcccaattg ctgcaccgaa tgctgttccg
    35461 gcgtctttgg atgttgctgc agtccagccg gtcgagcaaa cagacgaatt aagcactctg
    35521 aacaattata gcaaatcagg aaaacaaatc gccgccgaag ccttaacacg gcaaatccct
    35581 gacagcagcc cggctgttaa gaccgttatc agcaattcat ctaagccagt aaaagattgg
    35641 atgcctgttg tttcggattc tgtcccaaca acagatggca gcagaagcga taacctgcaa
    35701 aaaccgctgt ttacagggta cgaatccaga ctaacagctg gcaatgacag gatgccggcg
    35761 atgcattcgg attcccaatc agcaagactt cttgacagtc cggctgatca agaatcgacc
    35821 gaccagatga aatccgccgg tttatcggat gatataactc agctgcaccg gataagattt
    35881 aaacacaatg cgacaccgga caagattgcc ccagaaaaga tggtgcagga ttctgtacat
    35941 acagaacagc aaaaacaagg caccccaagc aaagcagtta atgctgaatc tgatgctcat
    36001 ccctcagttt taattgagag tagcaaaagt gaggatgaaa ataacctcat tttggctaaa
    36061 accgccgtac agaaattgaa tattgcagcc gagcagataa agcccttata tgctgaaaaa
    36121 tcgcaaacgg ccaaagacga atttcgtctc aagccagcgt ccgataacca taatctgatt
    36181 cagggttctg accttgtcgc tggtaaaaat cctgtccaaa cagaagacgg ccttccaaga
    36241 ccggtgcaat cgattcactc tttccttggt cttggccggc agattcagga atcggttgcg
    36301 gttttttatc gacacgggat gcgtcaaatt gtcatccggc ttgatccgcc tgaattgggt
    36361 cgggtgacga ttaaatttat tgaacaccat aacgatatta caggcattct ccatgtggag
    36421 aaatcccaga caaaacagga aatacagcag atactgcccg aacttctctt gaatcttcaa
    36481 agttccgatg tgtctattaa aaaactggag gttgctttaa gcagccagca gaataatgtc
    36541 gcccaagaac attctgcccg cgataatgga agcacagggc aggaatatca gcacaataca
    36601 gtaggcccct cttctgaaga gtggtcatcc cagaacgatg ctgggccgga acatggagac
    36661 ggcctttccc agatgcggct ggaagtctcg gacaaaacca tcaatctgct ggcatagcgg
    36721 gttggagtca atcgggtgtt ttgctgacat tcatttcagt attttcactg aaaatttgtt
    36781 ccttaacccc ctgtcttcct cttcattgga gccctttatc agtacaacaa agccgattat
    36841 tacagggcgt gccttcagca ttttctgcct tgccgatttt tagggaaaaa tacccttaag
    36901 ttttttgcgg tcccatcgag aataagtgca ggtgagaatt ttgtacttgt tcgtgtgttt
    36961 gataatcgct atcaagcagg gacagaaacg agtcattttt ttaaggagtt acaaacatgt
    37021 tagcaattaa aaacaacatt atggcaggca atgcagcacg gcatttgggc aaggcctacg
    37081 acagtctggc caagagcgtc gaacggcttt cttccggcct tcgcatcaac agctcccgag
    37141 atgatgcggc tggtctggcc gtgcgcgaac tgatgcgcgc tgatatcgct gtactccagc
    37201 agggtgcacg gaatgcccaa gacggtatca gcatgcttca gacgatggaa ggggctatgg
    37261 ccgtcatcga tgacaacctg gttcgcatga aagaactggc cgaacaggcc gccaccggct
    37321 cttattcaga tgcccagcgt gccattatga atgcggaatt tgccgaaatg gccgacgaaa
    37381 tcgaccgtat cgccgaagcc accaaattta atgacattgc catgctgaac tcctcaaccg
    37441 gttcggtttc tatccatgtg ggaaccagca ccaccattga tatcaataag gtcaatatga
    37501 ccaagagcgg cttgggcatc aataccggcg tagaagttga cgaagtcatc tttaagggca
    37561 ccgaatccgc aacaaccgat gatttcctga cggttgcaga taatgcggtt aatctggtta
    37621 tccagttcag caattccgca gatggaactt cggaagatgc cattactatt gatttagcca
    37681 caggctccta ttcaatggca gcagttgtat ctgccattaa tgcccagacg caggctcttg
    37741 gctatgcccc ggacggcacc gacaagagct ataatatggc ttctatcgtc accaacgatg
    37801 acggaaccta ttcgctcaaa ctgtcctctc gagatgaagc cgatgcagtt tcgattcaag
    37861 tagtgtcagc cacaaccgat cagcatggaa cggtctccgg actgggcggc actgcgacca
    37921 ccagcaccaa cgccggagct gatctgaata cagataacta cgatgaagca caagttgccg
    37981 gcggcgtcaa cattgcaaca gccgctgcgg ctgctgaggc cttggcaaca attaccgctg
    38041 ctatcaatac caaagaccag gcccgtgctt ctttcggcta taagatgaac cgtctggaaa
    38101 gcacgattga aattctggat attcaggccg aaaacctcag cacggcagaa tcacgcattt
    38161 ccgatgtcga tgttgccacc gaaatggctg ccctgacacg gacacaggta ttaagccagg
    38221 ccggcatcag catgcttgct caagcaaacc agatgccgca aatggctctc cagctgcttc
    38281 gctgatagcc gttaaaaaag ccggggagat gaagccgaca agggctgtct ccccggctct
    38341 ttgaaaatca ggagaggatg ctctattgac tctgaaggat tgaggaataa tgaagggact
    38401 tgaaacatat caggaaattg cagttacgac acaaagcgga ggacgtctgg tggtgatgct
    38461 ctatgacggc gccatcaaat ttctcaaacg ggccgtcaat gaactcaacg ccggcaatat
    38521 ggccgaaaaa ggtctttata tcaataaagc actggatatc atcaacgaac tgaataacgt
    38581 tttagatatg gatgccggcg gtgaaattgc tgccaatctc cgcacacttt atttgtttat
    38641 ggcacgccat ctcaattcgg caaatatcaa aaaagacccc cagatgattg gagatgtaat
    38701 ttccctgctg gaggagctga atcagggctg gaaagcgatt acccgcaatt cgactcttga
    38761 aagcgaaata tcccgcaggt ccgcagaata gccgacacag accttatccc tctcgtttgc
    38821 gcggggattg tttggaaagt accgactgca gagaggggac agggcatatc cagtggcaga
    38881 acgccggcat ctgaatgtct tgtaacccga tgccggcggt ttcgtctgca gtaaagtaaa
    38941 acacacccat aatcggctgg tcggtttcaa ttcggtccag tgtatgagct tgcggcatat
    39001 aaaacagtat ggtatcaaaa tgaccaagca ggtctgtttt gcccagaaag gcattcagtt
    39061 tgggaggcgg aacacagcag acccacagat tgtccagccc gctgggtaca atttttctct
    39121 gaatcagttc cggctccata tcaaagacac gagccgccgc atttcgctgg ttatccgtat
    39181 cggcaagcag aatccgccgc ttttgctgtg ccaagccgat agcagcattc actgccactg
    39241 taatcggcag tgtgccgggg ctttgtgaga ccagcccaat cagacgcggc tgggtgtttc
    39301 ctttgccgat aagtttcaca agctgttgat aatatgcttt gggatcccga cagggggtag
    39361 ccggccagac agggtttgaa ccgataacgg cgggttcttg cggttcgaat tcgacgatat
    39421 gctcttcgat tggctgctta acgggcggcg gctgtattgc ggagggccgc atatcgcttt
    39481 cggacccggg gccgacaaga ttggtctgga ttgcagaaat tgtcgcttcg gccccattaa
    39541 gccgcatcat caggctgttt ttgcccgcaa gaaaaccgca ggcggcggcc attgtcagca
    39601 acggcagtat agaaaaaccg gacaatgctg ctgcatccgg aacaaagccc attacggcag
    39661 ccggatagtg tcgagcagaa taaaccggaa ccagcagaca ggccagcaga atgaaaatgc
    39721 cgaccgcccc cgctgtcctc atcagaaccg cagatgaatg catgccggca aaaaaacggc
    39781 tttccagtaa aacacgtccg gccagaacca atgcctgctt ttcatcgaca gcaccaagcc
    39841 gcaaatccgt ttcgatcccc atttgcttaa gcggcaaaac ccggcggaaa taggtggttg
    39901 aagaaaggac aattttctgg ctgaaaacaa aaatagcaaa gacagcagcc atagatagaa
    39961 acaaacagac catcaatatc gccagcggct gactgaaaga taatgccgcc tctgcccagc
    40021 tgaacaatac acctgcctgc cgatcctcaa tgatgcatct tgcagttgac gattgcaccg
    40081 ccacaagcag caggatgacg ccccgcacga gggcggtaaa tccaataagt tcaaaagagt
    40141 gttttgccgc agcacagata actgcaattg ctccggccaa gcaaaataag cagatccaca
    40201 ggatgtccag aagtttattg ggcagaggta ttaggaaaaa agctgttagg accaatgctg
    40261 cgaccgcaaa cattgatttt atttctgttc tgccgttagg caaaaaagat gtacagtcct
    40321 tgtgtatatc cattgtctgc ttccatttat aatgtgcggt ctaagtataa cgcattattt
    40381 tgaaaaattc ctactgcaaa tgtttgtttg ggtatttctt tagatttcag attacacaag
    40441 acataccata cccaataata tttatattca ggtaaccccc aaccgctggt agaaaatagc
    40501 attttctttg tttgcctgaa cggatgatta tatacaactg cccaaaaaat gcgtttgaaa
    40561 taagaaaaaa tgttaaataa agaaattaac atgttaaaat aaatccatca aaaaataata
    40621 tttaaatata ttttataaat actgaaatat aaataagata aaaagtgtta aaaattaaaa
    40681 attaacgtta aatatatttt tttatttgat ttttattgcc gataaaggta ttatcttgct
    40741 tatgtcgatt gttgaagtgg caaaacttgc tggagtttcc cacacgactg tttcacgcgt
    40801 tctcaacaaa agcgggaagg tgaacacggc aaccgcagac aaggttcgga gggcgatgga
    40861 acaaatcggt tatgttccca aaccacctca tttaagacgc ggccccgctg caggttccaa
    40921 tcgcggattt acgacaaaaa accttgcttt tttaactgtc tctgaaaccc ttcgcattct
    40981 ttcaacatct ccttttttag tcaatttggt tcacggcctt caggaggcct catcggacaa
    41041 tggcctcagt ctttatcagg gcgtactcag tgcggatcgg ccgctgccgc cgtttattgc
    41101 cagaggtgaa gtggacgggc tgattatcta tccgggcctt gaaagtgttc cagaagaata
    41161 tgtgaaagcg atccagcggt atccggtggt ttttgttctg tccggcagag ataaatattt
    41221 cctcggcgac cgtgtgatgt gcaaccacga gcacattggc aaaattgccg ccgagtacct
    41281 aattggcaaa gggcgtcagc gtccggtttt tttcgacctt aaaaaaagtc ccaattatca
    41341 tcaggagttg ttcatgggac gatggaaccg ctttgaagaa gtctgccgac aggcaggaat
    41401 tcaagccaag cgggttgaaa ttcccctgac atctgaagaa accctgtttg acggaatcaa
    41461 ggtacaaaag acactgacga cagtggtgga aaacgttttt gtcaactgtt catccgatac
    41521 acgtccggac ggcgtgtttg tcgtcttcga cagcttgaca gcggttttat atccggttct
    41581 ccaatcctgc ggcatccgga ttggacagga tgcggacatt atttcctgca acaatgaagt
    41641 ctctcttctg gccggcttgc atccgcgtcc ggcgacaata gacctgcagc cggaaataat
    41701 cggccggcgt gcgtttgaac agctgcgcta tcggattcag aatcctaaag atcaaacacg
    41761 ggtaacgctg gagatattgc cgaaactgat tgaaggtttc ccccagtggc aaatggaaaa
    41821 acaggaatga gcagtaggca gacaacagcc ggaatgattc tgcttctgtc ttcttccggc
    41881 acggggagcc ggttgagccg gtacggctgc ccccgcaagg acaaggtgtc tttccaaaag
    41941 ctttaggcac aatttggtgc tgcatgaagg cgcctttatt attgaaagaa tagttgacaa
    42001 acagtgtatg cagttcctgg gccggagggt tggattggcg gcaatgacaa ttttcaggca
    42061 atcttgatgc tcaggggaaa aagagaagaa attatttttt ggaaagggta tggttatgaa
    42121 gtggaaatgt ctgtgttgga tggttgcagc gtttgtgccg gttgcggcgg cggatatact
    42181 ctataccgcc tatatcaatg ccgataccgg ctacctttat cgctatgacg acaatctgaa
    42241 tctgatttgg cagacttcca atcagcgggg tattcaccgt tttgcggtca gccccattaa
    42301 cgggaacatc tatgccggct atgatggaac agaccgatgg gtcaggcagt ttgaccggca
    42361 aacaggtgct ctgattggga ccacagtgcc cacaatctcc ggcctcagca gcaaccatgt
    42421 caatgatgtt gcattcggcc atgattggaa tctggatggt gtgccggatt tatggattgt
    42481 tacccgtacc tcgctgctgg ttttcaatgg tgcccagaca gtcggaagcg gagccgcgac
    42541 ggaacttgcc cgctggacgg ttgccgattc atcatcaacg gtttatgacg gtaccggcgg
    42601 ttcggcgatc cttttcggcc cggatatcac cggcgacggc gttgcagaac tctatgtcgg
    42661 aaaaggcggc aataacagct atggaaggat caatgtgtat aacatagcag ccagcacggt
    42721 ggacaatctg gtgaaggccg caacctattc ggccggcaat acccgggata ttgagaatct
    42781 gctgtttggt ccggatgttt tcggcaatga cggacatttg gatttggtag gcgtcagtgc
    42841 gcgaaactat cagctccgct gcttcaattt cctcaccggt gcggatatgg gagttattga
    42901 agaaggcctc aattcacggt attaccctgt tagtctggca ctgatgccga atggatcgtt
    42961 cctgctgggt acccgaatga agaccgaatt ggatcctctc tgggtcagcg gggcagaaac
    43021 aagcggaggc aacctggttc ggctggatcg tgtgcaaggc cctgctgttg cgtacacccc
    43081 aacgctgctg atgctgtcgc cgctgggcgg aacaaacgat taccggtttg ttgatgtcct
    43141 gtatcttgaa gaaaaggcgg cctatgcgcc ggttcctgct cccggcaaac gggtgccgtt
    43201 ggatctggct caaataagct ggacgaatcc tgaacccaat gaaccgtccg gggttattac
    43261 ctgtgatgtt tggttttcgg ccaattatcc tgaatatctg ccgttcccgg atccgaatct
    43321 gctgaaaata cccgacccga acgacagaac agcgtggtgg cttgaaaacc gcaattttac
    43381 aaattatgcg gccaaagttg ttagcaatca gccgattaat tcgctcaatc tggcttccgt
    43441 cacaacactg ccgctgacat atggacaaac gtattattgg cgtgttgata cacgggattc
    43501 atcgaatccc gaagctggaa caaccgttgg ccccgtgtgg atgtttacgg catacaactc
    43561 ggcaccgcag gttgatgccg gcccgaccat ctatacgtnn nnnnnnnntt accgttcaga
    43621 tggctcctgt ggtctcagac gacggcagac ccagccctcc cggcaaattg acgttcttgt
    43681 ggtcgctgca atcgggtaca tcggaggcgg ttacaatcaa cagccccgaa gtccaagata
    43741 cgaccattac tgttacagcg gcgggtacgt atgtgttcct gctgacagtc agtgacagcg
    43801 agctgaacaa tgccgacaca gttaccgttg ttgtttctgc ggatgcctgt gctgcggcca
    43861 agagcattcc cggttacgta cgcagagccg gcgacatcaa cgatgactgc caagtcaata
    43921 tcgccgacct ccagctgctg gcagctgatt ggctgaattc aacggcattg aagtaacggt
    43981 attcaacaga attaaagaag tacctgaagt aatgatgctt tccggggcgg tcgctatgac
    44041 ctccccggaa agttcacatg caagcatgca attttatttg gaggaagcgg aaaatgagaa
    44101 agtggaaagt agtattgatg acaatcggca ttctgctgat gctgcagact gttccggccg
    44161 caaccgttga ttttgatggc actgcgggcg acggcacctc gtgggaagat cccggcaact
    44221 ggagcggcgg tcccacaggt gctacgggct tgccgggcag cggcgattat gtggtcatct
    44281 ccggcgacat cgggggcgaa acgatcaata tggatgccgg cacgtggcag tatttcgtag
    44341 acaacagcct gctgaccggc gggtccgccg gcgagtaccg cacccagagc attcgtctgg
    44401 gctggaccca tacatcgact ctcaatgtgg atataggcga tggcaatatc tggcgcacca
    44461 ccaactcgac ccttcagggc gtcggctcgg ccagcggctc aaatggcaca tggaacatct
    44521 acagcggcga catctgcatc gagtccagca gtttccgaat cgcggaaaaa gccgggtcag
    44581 caggactggt caacatcgcc ggcagtgcga acaccagact gattatccat cgcgagaccg
    44641 gcggagtcag tatgcaggtt ggcaccggcg gcgctggcat attccagatt gacagcggca
    44701 agtttcgcac ccgcgctggt gtaaccgtcg gatcgaacgg cctgtttcgt gttctgggga
    44761 gcgacgttac agaagtttcc atcggcgatg agagcacgct tgacggcaac tggactcaga
    44821 atgccggcgg cattctggaa ttcggcattg atgcaggcgg tgttacacct atcgtgattg
    44881 ccgacaaggg cggctccggc acctttgcaa tctttgacga tggctcgctg ctgaatcttc
    44941 atttccttga tgctcccata gccggcacat ggactcttat ggaagtcaag aacggtttca
    45001 ttaccgataa cggcttgatg cttgctgccg gcgtagatcc gggctggtct ttcagcatcg
    45061 ataacagcgg ccccaacggt cttctgactg ccgcttatgt tccggaacca gcggcggcga
    45121 cattgcttgg ttttggctcc ttgattctat tgagaaaaag aaaataagag tacccgcacg
    45181 gtccggccgg cattttagac cggccggact gcctgcataa taaaaggaga aatcagctga
    45241 catgagatgc cgaataagca gacagggatt taccttaatt gagctgcttg tggtaattgc
    45301 gatcattgga ttgctgctgt cgattattct cccttcgctt cggaaagtca aagaatctgc
    45361 ccgccttttg gtctgtgcaa ccaatctgcg gagcatccac gccgcgctgg caatgtatgc
    45421 cgaaaacaat aatgataaag tcagcgatcc ccgcggggat acaacagcca gtcccgatcc
    45481 gcgtcccaat ccggcggttt actggaaagg atatgcttat gatcgatggt gccgcaagtg
    45541 gtatctgcgg ttttatgact acctcgaaac gccggaagtc tatgtatgtc cctcgtggac
    45601 aaaaagagaa ggcgacagct atattgctta cgctgtcggg gacgacatct attatgtgac
    45661 ctatacagga aacgaatata tcctcagttt aagagagcac gagagtcaat caggcggcgt
    45721 gcgaaaacct catgagtgga aatacacaga acttgttcgc aaagctgtcg gcaacaatcc
    45781 gatggcgttg tttctggcag acggctttta tgaggttaac ggatggggca actggaaagc
    45841 atggcagcga gaaggtgtaa tggccccggg cgggtctgga agagccagtt atcgccatgg
    45901 ccgacgggct aattttcttg tagccgacgg ccgtatcggc tgtctgagta tggaacaggt
    45961 cgattcttgg agcggccacg gttcatatga tgaattcaaa ccgtctgaac ttaaataaat
    46021 aggtgaattc gaagcagtgt tgcctgtgtg cgggggatca agatgcgcag atattgtatt
    46081 ctattagcat ttttggctat gtgcggattg tgccggggcg atgtgttaga tggtattgtc
    46141 ttcggagata cgcattcgga atccgctcat tctgttttgg cgcctatgac cgcgatagag
    46201 acaggcggac tggggcagcc ctgcagacgg ctcagcgcca gcggttccat tacatttacg
    46261 cttgcctgtg atcccgtcag gcagaattac gtaacggtca aactgtgggg ttcggatgtt
    46321 gtcaacggaa cacagtattt atatttgtac gatgcggccg gcagaatcgg cacgtatcag
    46381 gatgactggc ccgaaatagc cgtctggacc agtcagccgc cgtttcccgg ccggtttgtg
    46441 tattctactt atctgattcc caaacagtta acatccgcaa agacgcaggt aaccctcaca
    46501 ttggccgatg cccagctcgg gatttatggt gtatattctc atgtggatgc tttttttaat
    46561 ccgctcggcg aagttcacgg gcagcatccg gggcccggtc ctgttcggcc tgccggctgg
    46621 cgcagtcctt atgaccagct ggtttatcag gccaacctcg gtattgcaga atttctcact
    46681 tggcagaaat atggtcctgc atggaatgcc gccgtggccg ccggcaatgc tccggcggtg
    46741 attacgggag cgattacctt tgatggacgc ggcggcagcc cctcttggac ggttcagcag
    46801 tggaaggaag atatttatgc ccgatttacc ggcggcaacc ttgtctgtct gcaggcactg
    46861 gaggcctacg ctgttgctta caacagccct tggtcaaatt attatcgcaa tcccgagctg
    46921 ttagaccgaa tcgttaaagg actggacttt ttcagaattg ctcagggagc caacggtgca
    46981 tttggaaatc cgtggggact gctttggatt ggcggcccca acagggtcaa cggtgcacac
    47041 tgtctggaag gttttggcgt tcatgcactg gctgaagcat ttttgcagat tcacagctat
    47101 atcagcccgg cggtcatgaa tgaactggtt gatgaggatg acaatcccga cacgccgcct
    47161 gtaacgcgca aaacatctta tatcaatctc ttcaaaggca ttattaacca tatgctcacc
    47221 gaccgagggc atgctcctaa tcaggatcgg gcgcaggttc atgctatgta caaatcaaat
    47281 cagtgcctaa agattttatc cccggcagag gcttggcctg aaagtacagc ccggcagtgg
    47341 atttatgaag cgatcggtgc tctgccgtgt ccagtgtatg gcggatattg ggaatcgcaa
    47401 aaagggctgg cgctggaagt ccacggaacc atcaacggcg gctactgcgg cagttatggt
    47461 actgctgcga ttagttttat ctatccttat ggagtcatga caggagggca agatgctctt
    47521 gttaacagca tcttcagcaa ggaagtcgat gcttttgcca attatcttta tcaggacaat
    47581 gacagcgacg gctttgccgg cttggtcaat gaatccgtta tcagctggcg gcctaacaaa
    47641 taccccggcg tacagttccc ctcacccggc gctttcaacg ggctggccta tgcagcgctg
    47701 tatttaaaca accaaaatgc tgtccggata ctccagaagt atttagaaaa tcgcctcctt
    47761 tacaccctcg actgttcggc atcggcagtc gccgcccact atgaggataa taccattgaa
    47821 acaatcaaaa tggtcaatct tttcaaacag gtcgaggcac ttgccccgac aaatgcacgt
    47881 ctcccgatgg aagcgggcga tacctttgcc tgggctgatg agcaggccgc cggacttgcg
    47941 gtatatcata acggcagccg cctttatatg acccttaact ggcggcatga ttactgctcc
    48001 ggctgcagcc gttcagccgc caatgcaata gccaacaata ttgcacgcat ccattataca
    48061 accgatacaa tcgatcgtat tgccaatgtc cagatgaaaa ctccctatgg attgtttaag
    48121 ctctatgtct gcagatatgg cgactatctg attgcaatga atctgtctga cacagagtct
    48181 tttccggtgg atacctctga tttgccgtgg acggatgcgg tggatttgat tggaaaagtg
    48241 cgaatgaatc ttaacgccgg tcttgttatc ccgcctcgtt caacgatgat tctgtatccc
    48301 ggccgctgca tggactactc agccggcgat ataaatagag actgtgtagt caatctggat
    48361 gatttttcga tacttgcggc ccaatggctg gacaatacga atttgcaata gacaagcaat
    48421 gcacaagcag cagtatcgaa agtgccaaac gaagtttttt gtgccggctg aatctgtctg
    48481 cagaaagcag acctgtcggc aggagctgga aatattaccg gaatctggta tgcttagtat
    48541 ctgcaagaca tagacctgtg cggcagccgg ttttggaata aaagaaaacg atgaggcaaa
    48601 aagtacagat tgtatatctt attgttatcg gcgtgtctgt cggacttctg catgcggcct
    48661 ttcatcaccc gggtctgctt cacagccgac agcagctgga ttttattaag aatgcagtcg
    48721 cagccggaca gcagccttgg ctgaatggtt ataagcagct cgctgctcac ccgcaggcca
    48781 gttatcagta cactgttaac ggcggttatc agaccgtagg ccggggaaga aacagaacag
    48841 acaatgtcca taaggccgaa ttcgatgcag actgcaatgc agcccattat aatgccctga
    48901 tgtggtattt gacagacgat cggcggcacg ccgacaaagc catcgaaatc ctgaatgcct
    48961 atgcccgcac gctcaaagaa atcatcggta cagacaaaat tcttatggcc tccctcaatg
    49021 gggcaaagct gacgtatgcg gcagaactga tgcgctatac caatgcggga tgggattctg
    49081 ccgacagtca gcaattcgaa actctgctgt tgacggtctt ttatcctgtg attcgggact
    49141 ttgctccatt tgccaacggc aattggagca cgggctgtgt gaagacaatg atggccatag
    49201 gagttttctg tgaacggcag gatatctttg accgggcggc ggactggtac cgcagcggaa
    49261 ccgataacgg ttctgtgctg cattatatca gtgaaaatgg tcaatgtcag gaaagcgggc
    49321 gtgaccagca gcatgcccag ctggggcttg ctcatctggc agaagcctgt gagattgcat
    49381 ggaatcaggg tttggattta tatggctggg cggataaccg cttgctgaag ggatttgaat
    49441 atacggcccg atacaatttg ggctatgagg tgccgtttgt gcccttgacg gataggaccg
    49501 gccggtatta tcattcagcc atatccgaag caggcagggg caatctgcgg cctgtgtggg
    49561 aaatggttta caatcattac caaaaacgca ggggactcga ctgtccttat gtccggcagg
    49621 ccgctgaaaa acttcgccct gagggagcag gtccttatgc tgactgctgc ggatttggaa
    49681 cgcttctttt taccctgcct gccgatcgta cccaaagccg gcagtctgac tcggctggtt
    49741 ttgaaaactg gaccggattt aagcattgtg agcaggccgc cgaaggcgga caagctgttt
    49801 ttacgctgac cattccgggc agagccgaat gtgttacaaa ccgctggata gagcttggcg
    49861 gctgggaaat gcccgggctg gatgccattc ccgaaagccg cctgaatgcg gcagacctga
    49921 gagaatacca gtcggtctgt tttaccgccg tgctttctga cgacagatcg gttcgccttc
    49981 ggttcagcgt ttggccccat gctgtttcac gcggcgaata cccgaaagaa gtcgcttcga
    50041 cggttgttgt ttcgggcaag ggaccgcatg acattgtcct gccgctggaa cagtttgaat
    50101 atcctaaggc gatgggggct ttctggaaat ttattcagaa aatatcgttg tcggccgagt
    50161 atgtgcatcc ggcagatgca ggccgactgc aaataagcgg cctgcggctg agccggggcg
    50221 agctgataaa gctttcagcc cctgttaaat ccaagccctg ccagccgggg cagacggttc
    50281 aatatgggct tacggctgaa aatctaacag atcggcgcct gcaagtaaga ctttttactg
    50341 aatcgtatgg atgggaagcc ctgcaacccg aactttcgac gcttgttttg cagttagaac
    50401 cgcacgcttc cgaaatggtt tctgtatctg ttacgatgac tgacgatgtt gccccgggcg
    50461 gccgccagac ccatatgatt actgccgttg ctgac
//
LOCUS       DAOTBO010000013        49306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_583_length_49306_cov_5.792329, whole genome shotgun sequence.
ACCESSION   DAOTBO010000013 DAOTBO010000000
VERSION     DAOTBO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 49306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49306
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_583_length_49306_cov_5.792329"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="thiL"
                     /locus_tag="PLE88_02890"
     CDS             <1..854
                     /gene="thiL"
                     /locus_tag="PLE88_02890"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOM75444.1"
                     /translation="FPIGIGDDMAQVRVGEDGTVLITTDMLLDGVHFDLCRCSLRQAG
                     YKAMACSLSDCAAMATVPLCAVAAAALPPDFGQAELKELYTGLTQAGRAYDCELIGGD
                     ITKWRSKESRFAINVAMISRPSGFHPPVRRNGAKPGDYICVTGSLGGSLAGKHLYFTP
                     RVNEALQITEKAVIHSMMDITDGLSTDLNRICKQSRVGAIVEAANIPVSKAAQKTSNP
                     LEAALHDGEDFELLFTLAPEEFQKLPSIPAVPIVKIGTITDSGRLEMKYADGKVVVLT
                     PRGYDHL"
     gene            863..1333
                     /gene="tsaE"
                     /locus_tag="PLE88_02895"
     CDS             863..1333
                     /gene="tsaE"
                     /locus_tag="PLE88_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOM75445.1"
                     /translation="MMSKGPIEFITHNAAETIAFGRRIGAGLRGGEIIALVGDLGTGK
                     THLVKGIVLGLDASDAGEVTSPTFVLVHEYLGCGGAKTIYHIDAYRIESTAEFEALGF
                     DEYCRPDTIVLVEWADKVLSALNGYDVIQLRLEHIGKSKRRISVYNPPDFLSAK"
     gene            1794..2249
                     /locus_tag="PLE88_02900"
     CDS             1794..2249
                     /locus_tag="PLE88_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75446.1"
                     /translation="MRKNKGFTLVEILIVVVILGILAAIVIPQFSDASTQSKVSSAKS
                     SLQTLRSQISLYKIQHNDNPPALATFAAQMTGKTDIDGAAGTQYGPYMQAVPKNPFND
                     SDTLAAADGATVGWVYDETTGIIKLGGQGATDLNGDGDTADAGETHIDW"
     gene            2411..2878
                     /locus_tag="PLE88_02905"
     CDS             2411..2878
                     /locus_tag="PLE88_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75447.1"
                     /translation="MKAGKENPRKLTKLDRCVLIGLVGTAVFLLVPPLIQAGQEKKLS
                     KMVDRLQIIRSQISLYKADHNGLLPGQSAPGQSICPEDILLAMMQSRPNGSGPYLRQI
                     PENPYLAETGRGTMITCVNDPNAQPTGAEGTGWWFNAATGQWKACDSEFHANY"
     gene            2944..3351
                     /locus_tag="PLE88_02910"
     CDS             2944..3351
                     /locus_tag="PLE88_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75448.1"
                     /translation="MMYGKTKGFTLLEATMAMVIVAIAAAGVLLPFAQAASVHAEAAR
                     QTMAANLASEMMEIIAASPYDTIMDYNGQIENPGQLKDSEGNYHTGSAYEGFGRSVLC
                     QHVGSVNLAKVTVVVTYRNAPVTRITTLIGNHR"
     gene            3378..3884
                     /locus_tag="PLE88_02915"
     CDS             3378..3884
                     /locus_tag="PLE88_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75449.1"
                     /translation="MKRRMHQYGFTLVELIIALMIASVVMAAVVTLANAAVNANQATD
                     LMGREQAQLRLVLIRLTDLIRRANQVTAADIDSFQLWHDNNADGLKTADELTQISRGT
                     GGNTLQIDSIDSSEVYTQCRNISFDYDSAAPQTRKVTIWFDITAAGQSQRYSIVARLR
                     ASDEHQKL"
     gene            3923..4390
                     /locus_tag="PLE88_02920"
     CDS             3923..4390
                     /locus_tag="PLE88_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75450.1"
                     /translation="MDEMKSYKRRFSSQRGMAMLAVLFVVIMIAIVTSDFIARSDASL
                     ASGRNLCIRNEVDYAAWGALEAAWALMQDPNVRAALPYSAVAQQLDDTSQIYYELSIE
                     CCGADPNLYPVECRAYKRVNADIKARSVQSVLKADLKVDPNSMQIWYASIRRQ"
     gene            4561..5094
                     /locus_tag="PLE88_02925"
     CDS             4561..5094
                     /locus_tag="PLE88_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75451.1"
                     /translation="MKKAITQQNRLTAAGFTLIELILIMVIIGIAALIAIPTISSAAD
                     VQVRSAANRLAADLDYAKGLAITHQRLCAVVFYPNSESYDIRWTDTDEIVEHPTQPGR
                     NFVVNFVSEGILKRVNLDSTNLDSNTITFDYLGSPYNGKGTGQPLNSGRITLKADAFT
                     LYVDVEPVTGYVTINDS"
     gene            5122..6252
                     /gene="pilM"
                     /locus_tag="PLE88_02930"
     CDS             5122..6252
                     /gene="pilM"
                     /locus_tag="PLE88_02930"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOM75452.1"
                     /translation="MLQNWLKPKSLPIGLDVGHSMIKMIQLCCKDGYISVEAAEEEML
                     DRTLAPDSDEWRRHTADLITAMYRRGGFRGRKVVSCLPGDAVKIKSLRLDTVEPEEIE
                     KIMHTEVSRRFGLDPQRDEIRYMVAGNAYQGEEIKNEVIFFGIESRQVTNHIMLLEEA
                     DLVPISLDTVPCALFRCFQATLRRREDQDLVSVFVDLGSQYTTVIIGKGQEIAFVKQI
                     PIAGNALNEQVALRLGISLEEAAAVRARLRSSDDQQKNPETRRAVIDAMSTSIEMLAH
                     EISLCFRYYAVTFRGRRPAEAAFAGGEAYESALIDALQRHLGVEIRIAEPLRGYDLSR
                     AKFDRRRNPQMCEWAIAVGLALKGMELNQSRQYAAQETEVSV"
     gene            6249..6905
                     /locus_tag="PLE88_02935"
     CDS             6249..6905
                     /locus_tag="PLE88_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOM75453.1"
                     /translation="MKEIDFIPEWYKADRRRRQRYIQQRTLLGIIFAVMVVWSFVMGQ
                     HVKRVQAEMNSVQTAFELGKARVEQVHELEQQIAVMEQKTQLLGYIAPRTKISALMGE
                     ISYLAGSNILLSRLCFQNEPLQSPKSVVQHSAAVVQTAAAASSQSWERQQVPLRIKTT
                     LTGIAASGADAAGLISSLEKSDYFEQVVPVFTRAKKVGDTEVIEFEIRCYVADYRIQQ
                     "
     gene            6917..7465
                     /gene="pilO"
                     /locus_tag="PLE88_02940"
     CDS             6917..7465
                     /gene="pilO"
                     /locus_tag="PLE88_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOM75454.1"
                     /translation="MKNLDKQQTAILILAAIVIAGFAGFRYVPLVRQKYALDVQVKMQ
                     KQTIEQIAASSALLPELKQQHKLMTERLEAFGRKIPEGRNFANLWQQIADMMNACRLT
                     DQVVQPGAELTSETVCCIPLTIECKGTAEQMFAFFQSLENMDRLIRMDEVRFENDGNY
                     SGIVKLTAKASVYYQPSAVRSG"
     gene            7472..8068
                     /locus_tag="PLE88_02945"
     CDS             7472..8068
                     /locus_tag="PLE88_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75455.1"
                     /translation="MNRKTPSLARKIQTPASLQNISPKMMVMVVLLAVMGILWGRVLL
                     GGKKGPAVAEAQQAAVSAAELQSVQSAPAIRINPVELPCRLGRNDQLTNDLFSRKGFL
                     SAGAQSSSRDSTADKQSRLERLATAQLTLEAVVRREDGTPYQAFVNGKIVTVGAMLTV
                     NEGPDQYVLTVTDIKENRVELEWNTVSVVLKMTGTSNQ"
     gene            8084..9745
                     /locus_tag="PLE88_02950"
     CDS             8084..9745
                     /locus_tag="PLE88_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75456.1"
                     /translation="MLAKQNRNAGKTKLAAAGVWILTAAVFAALAAAEEPAAAPEEAA
                     VVQQGVMGQSIQTITFKKDMPIRDALAMLAQMYQKNIVPSAAVDGIVTVSNLYDVTFE
                     EALQAILGTHKYEIKGNFIKVYTNQEFMQDKTRFEHAIIALHYINSEEARKLAEPLLS
                     EFGKLGVTTAAPAEMQAGKGGDTLAVHDRLVVSDYPENIQKIRDILAEVDVEPLQVLL
                     EVTVMEATLTDDTKFGLDWKNISDTSISLGGKGFIQSGFTPSTSDTGGLSVGVTFDNI
                     SALITAIETVSDITIMANPKILALNKQTGKIIIGNQDGYLSLTNVAEGGTSTQQVEFL
                     ESGTVLEYRPFIGRDGMIRMEIHPEQSSGEIKVIGGATLPKKSTTEVITNVMVQDGQT
                     IVLGGLFKEQTTQSHSQVPVLGDIPVVGELFRGINDKSERVELIVLITPHIINTPKEA
                     DGADRLEDVMRLAHNARQNLYWMSRAKIEEDRYAKAVKLYAEGQPEAALAMLDHPYAI
                     NRNYLDEVRLKERIIRETQPDKVNQIERIMLRKIENEESGKWFRR"
     gene            9836..11041
                     /locus_tag="PLE88_02955"
     CDS             9836..11041
                     /locus_tag="PLE88_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75457.1"
                     /translation="METGKTVGKLMLWAVGMLVCAAGCAPSHQQNKQAMTQRWEQTSA
                     QANIPQAEELISRGQLDKARKILNACLDVSSPQPAVYFLMGQIDMMEGRTEQARQNLQ
                     QAVQLDPELDKGWYFLGTLAVLDKDYVKAMEYTQKALDLQPANTDYILTLADICIEGG
                     RIEQAEKILDQGLKLQSGNLDLMLAKARLLQQSGLRTQALLLYEQALLMHGAVAAILE
                     PCAYAYLAEGQWQQAADKFEQLLPLYKQDLDRYNTTMRSLALALFNAGRYGQALVYYD
                     KLSVVYRQDADIWLMMAHSAMGVDDAKRALQCAQQALKYHPSWASAYAVSGCARYFLG
                     EYEQAVQDYAKIINHEELGAFAWFMTGRCYQQLGQPIQANAAYERAETINPNNELIKS
                     FLERTLKSL"
     gene            11057..12484
                     /locus_tag="PLE88_02960"
     CDS             11057..12484
                     /locus_tag="PLE88_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75458.1"
                     /translation="MKKDQFIWIAASAAGLAAGGAAILAAVLSGSVSVILLAIVWLSI
                     ALTGGMLLHHIHQLKALERVFRVGSQMKIPFEIVPGSVLGDIANAANCLLDETHQKIR
                     QMQDENRDLMLQMQLLRRRKSGIEAVLNSIQDGVLVTDDKDRVLLANPAAQNLLGFEF
                     NKDNACLIQDAVNWPELVQLIVKSRQSKIPHVRHELSRQKDGRPMTFDSVLSCVEDDV
                     GNVLQIVTVLHDITREKEISQMKNDFVSHVSHELKTPLASINAYAEMLVDGEAQDAET
                     MRQFCSVIQGQAQRLSRLIEDILNISRIESGLVKVSRQNHSMALVIQDAVDMIVSYAQ
                     EKNISVEAPAPILYDQVYIDRDMISQVIINLLSNAVKYTPSGGQIAIRSEINELDGTI
                     TVTVTDTGVGIPEKDLPYVFDKFYRVEANNKYAKGTGLGLNLVKQIVESVHGGSVFAS
                     STPGKGSTFGFILPLAKAAAAQMVS"
     gene            12526..12912
                     /locus_tag="PLE88_02965"
     CDS             12526..12912
                     /locus_tag="PLE88_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM75459.1"
                     /translation="MEKKKALIVDDEFHIVQVVAIKLRNNGFDVATAENGSQAYDLAC
                     QTKPDIIVTDYQMPIMNGLELIEKLRQNPQTAAIPVIMLTARGFSIEDRLQEQLRVSA
                     CLSKPFSPREVLGIIEEVLKGQNVAV"
     gene            12949..14538
                     /locus_tag="PLE88_02970"
     CDS             12949..14538
                     /locus_tag="PLE88_02970"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOM75460.1"
                     /translation="MTHNASSQALTDEKIYPFQSLADKLNRLDLNLLLYDAGGRCVLQ
                     SSAGRFESDTEAIAGQIESMQTAQNGLLLSGWPSDNLFVCPVSIEPGLTVFLAVDTGP
                     AGCRNAVCEKRYLACIVEDFCKQFQDAERIPMQLEKIGTELSRAYEEIMLLYRLGANM
                     KVTQTNASYLQMACDQLTQLVHVEGIVFFVEKKIDGRRQLALIAGSGVISIEPSQADI
                     LQTRLIAELQRGREALVDSAEDSPFRYDWPPAVRNIIAVPLGDADRMLGFMAATNILD
                     KRDFDTTDIKLFGSVANQCRVFIENNSLFEELKELFIGCLKALTGSIDAKDQYTRGHS
                     ERVACISRWIAERLAQHRPLSDKQIHHIYLAGLLHDIGKIGIGESVLRKRGRLDDNEF
                     AVIQSHPRIGASILSGIRQMDEIVPGVLGHHERMDGKGYPNGLKGDQIPLIAKIISLA
                     DAFDAMTSRRVYRDAMTIQKALSEIEKGIGTQFDEEVARAFLESDVDKLWTIIQDGFI
                     ESWDYSNFEEYGIEAVGALIR"
     gene            14535..14870
                     /locus_tag="PLE88_02975"
     CDS             14535..14870
                     /locus_tag="PLE88_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM75461.1"
                     /translation="MKIESQKYNDVVVLQLQGELTGDALKPFEDEVSNALAARISGIV
                     LDMSKVVFIDSCALEFLTDLNDKCRERTRQMKIAGLDETCAKILEITRLLNQFDTYGE
                     LTEAVKSFV"
     gene            14889..16604
                     /locus_tag="PLE88_02980"
     CDS             14889..16604
                     /locus_tag="PLE88_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOM75462.1"
                     /translation="MNTTVQTRLPLGQVLLNRGVVTQEQVDQALSRQQVIGHSKLLGE
                     LLVELGFCTDNQICSALAEAYGVPYAQLTPKLCDPAIVELLPRDFLEEHVVLPLFKVH
                     DMLTVALSEPSNVFVIEEIQRLSGCRVQIVCATARDIRATLQNYLPAANVFVIDDIID
                     DVALEDFAMIENLPQDIADLEEVAGQSPVVKLVNYLIYTAVHENASDIHIEPDEKKLR
                     VRYRVDGQLYEKLRPPHQMHAAIVSRIKIMAELDIAQRRLPQDGSIHVLVQSKPVDLR
                     VSVMPGIYGEKVVIRVIDFRKMLTNLESLGFGYDNLKLFKKVISRPNGIVLVTGPTGS
                     GKNTTLYAALSELNSEQVNICTVEDPVECNIHGINQFEVHEMAGFTFSSALRSLLRQD
                     PDVIMVGEIRDQDTANIAVQAALTGHLVLSTLHTNDAPGAITRLIDLGVAPYLVSASL
                     VAVLAQRLVRKICPNCKEEYEPAHSIRRTVKEWTGQDIVFYRGLGCKKCRNTGFIGRI
                     AIHELFVPDDRILDMITQNVPLKALRAAAIENGMVPLHIDGIEKVKAGITTIDEILRV
                     ANVMD"
     gene            16647..17798
                     /locus_tag="PLE88_02985"
     CDS             16647..17798
                     /locus_tag="PLE88_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM75463.1"
                     /translation="MKSKQHPAAETSAAGGTFFTGRLSNLPAPASHVRAKPSELMMFT
                     TQLSVMLDSGVVLSDAIEAIAEQMNPGTFQQVVLEIAQRLRNGESFSSALQNWPRIFD
                     TMYISMVRTSEASGKMAEMLDILSGYLNADAETRKQVKSAMIYPMVMLLMAVAATGSL
                     MFFVLPRFTRIYQARGAALPKLTQVLVNCSGLLANSTFLAAAVTAGFLGFWAWSVWRQ
                     TESGRKIIDWIKVHTPIFGTMITDSIMTRNMRILATMVNTGVSLLDALEVMKTSSDNY
                     YFQILWTHTDAKIRDGYQFSDAIQMSPCSGLIAPAITQMLRAGEKSGQIGQVCDKISV
                     FLAKKLQNSIRTATALIEPLMITLMGLIIGTIAIALLLPVFRISSVMSH"
     gene            complement(17900..19036)
                     /gene="ribD"
                     /locus_tag="PLE88_02990"
     CDS             complement(17900..19036)
                     /gene="ribD"
                     /locus_tag="PLE88_02990"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOM75464.1"
                     /translation="MEKDRYEDHFDFMKQALELARKGLGEVEPNPAVGCVLVRKGTVI
                     GRGWHERFGGPHAEINALADCRKQGHTPEGAVMYVTLEPCTHVGKTPPCCKAIIEAGI
                     QRVVIASEDPTPLAGGGIRQLSEAGIEVHTGLCRQEAEMLNAPFYKHARTGLPWIILK
                     WAQSIDGKLAWKSMHGKRWISGEKSRQKVHHLRKKVQGILTGVDTVIADNPDLTVRLQ
                     DVRVERPPLRIVLDSRLRIPAESHLLTKTDAPTLVVTTQETAQSAQNRIASLKKAGIE
                     VLTVPQQHGRCDLKNTLSQLGQRGIQQLLVEAGPHLIGQFLAHQLADEVCIFIAPLIL
                     GTDGTAELGLVLSQTAGYQTLQAVQIEQFDSDICISATLQTHPF"
     gene            complement(19076..20701)
                     /locus_tag="PLE88_02995"
     CDS             complement(19076..20701)
                     /locus_tag="PLE88_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM75465.1"
                     /translation="MEKKWVNMQILCWVLLLGTGCGEYSAPFSPRQAAPEKVQIIEPL
                     DLESYRSAEQPAVPSVLEAPKEPPASLTLALEQCRSQALENNLDIKVQLLAPRIAEEG
                     VNAERAKFEAAFFSNLSYSKTDQPPAAALAIQGSQIDSGYVDLGVRIPLQTGGTITFD
                     MADSRTKSNFQSATYNPSYSEDFSLSISQPLLRNAGIRTNTYSIRIAAYDKSIIDART
                     KLEVIRVIAAVDRVYWRLYAAFKQLEVRKQQYDLAQAQLERARRFVEAGQHPPIEIVR
                     AEAGLAQSLEAIIIAENNLRDRQRELKLALQQKDLQPAGPTVILPATEPNPVHYTFSA
                     PTLVSQAIENRMEMLEQELQIAKSISTIDYLRNQALPLVTLDYTYNINGLGPARSAAW
                     DMLGRNNFADHRLGFQLMVPLGNEAAESRLRQAFFQRQQALATKENRQAMIEVEVLNA
                     LDQCEANWQRILASRQSALLEERLYQAEIRQFEIGLRTSTDVLAAQANFANAQSAEIN
                     ALTEYQISLVDLAYATGTLLGSAQVEWAPASLK"
     gene            complement(20718..21980)
                     /locus_tag="PLE88_03000"
     CDS             complement(20718..21980)
                     /locus_tag="PLE88_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM75466.1"
                     /translation="MRFLLRTPLVILRLLIQSTLLAINQIWVNKTRSILTTLGIIIGV
                     ASVSAVIAAMDGMNKKIMENFEIFGTKKIFLWPQRPSTGPKKNTPWWDIRFRTQEFEG
                     LLEHCPSVEYLSCVSDTQNINARYADRTVESVRLTGVEASWFKIENRNIVMGRPFSFL
                     DETQGRKVCIITADLRDKLNLDNNCIGQSILLGYTPYLIIGLLEDKPAMMFGNMRENQ
                     YEVFVPFRTINKTDFQWFRVIASCKNAEVAEEAKAEIQFFLRRSRNIKPGEPDTFGVF
                     LMESEVQQFKKVMGMITLVAFGIVSISLLVGGVGIMNIMLVSVSERTREIGLRKAVGA
                     KPSAILTQFLVESVVLCMIGGAIGVFLGHGFAKLISKSAPLMEQTQIPAWAIVLSFGF
                     AGAVGIIFGMFPAIKAARLNPIDALRHE"
     gene            complement(21977..22696)
                     /locus_tag="PLE88_03005"
     CDS             complement(21977..22696)
                     /locus_tag="PLE88_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM75467.1"
                     /translation="MSNTLASSILIRVRNLTRTYKVGADRVYALRGINLDIKTNEFVA
                     IMGHSGSGKSTLMNLLGCLDRADSGTYELNGIDTTKMTASQLAHIRNQQIGFVFQSFE
                     LLPRMSALKNVELPLIYSDVSFLRRRKLAMQALERVGLAERAHHKPNQLSGGEKQRVA
                     VARALVGSPSLLLADEPTGNLDSKTSEGIMQLFEKLHQEGQTIIMVTHEADIAAHARR
                     IVAMKDGQIFSDQQASKGADA"
     gene            complement(22693..24072)
                     /locus_tag="PLE88_03010"
     CDS             complement(22693..24072)
                     /locus_tag="PLE88_03010"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM75468.1"
                     /translation="MSKKVFIVLCIVVVLLLILSAGIIKKRKTDIQSIQVRLETVQPS
                     EFVEIVNAPGEIRPKTKVDISAKVSARIEELPFEEGDTVTAGDPAANPPVPADVLIRL
                     DSRDLESQLRSAEAGRDAQRALIEVEKARLLSQQANLEGLKASLDSAKLEYERQKKLL
                     ETKDISQSSFEQTESSFRQMQAQFDAAVHSLEAAKLNLVVMEHNLSAAEARVEEVREA
                     LSYTTITAPIDGVITQINAGVGEVVMTGTMNNPGTVIMQVADLSTMILLAELDETNVG
                     QVKIGQRAKIHVPAFWDVEFQGVVRNIALTHRMSSIGAKYYKTEILIEGEVGKLYSGL
                     TADVDIETNRYENVIKVPSQAVLARRVDELPLDIIDNNPVVDAKKTEIPVVFRYINKK
                     AVATPVHIGPSDLTHTLIREGLQPGDAIIIGPYKILETLKHDTAVTEEKKKEAADSDI
                     TAAKNKKSL"
     gene            24250..24546
                     /locus_tag="PLE88_03015"
     CDS             24250..24546
                     /locus_tag="PLE88_03015"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM75469.1"
                     /translation="MTYEAAQYISEILKAVGHPVRLQIIEQLRDGPMCVGLLSQKLDM
                     PQSVISQQLGLMKDKQVLQCRREGTKVFYSIKNLNVIKLLDCVYSQCPAPEQSA"
     gene            24624..27485
                     /locus_tag="PLE88_03020"
     CDS             24624..27485
                     /locus_tag="PLE88_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOM75470.1"
                     /translation="MKVFSESVVQWSVAHYKLVTAAMIVFTLCCGAFIPLIQVDTDPE
                     NMLSPDEAVRVFHNQTKKQFDLSDIVVLGIINERNEYGVFNPATLQRIYELTEFAKTL
                     NHLDKKDPQRLAGVIEVDMIAPSLIDHIGQGGPGVITFEWLMPQPPQTQAEALEIRDK
                     ALSNPLLNGTMVSEDGKALCLYIPLTDKHFSYRVYSELNKKIAQMGGEENFYITGLPV
                     AEDTFGVEMFIQMAVSAPLAMLTIFILMLIFFRKLILVIAPMIIAMVSVISTMGLLIG
                     FRYPVHIMSSMIPIFLMPIAVVDSVHILSEFFEVYTKQKGRRQAVLDVMQVLFAPMLY
                     TSLTSAAGFLSLALTPIPPVQVFGVFVAVGIMIAWIFTVTFIPAYVMLIPEKFLATFG
                     RAVSDEDKPGIMTRLLHSAGGLTCRYAKPILAALAVLTGLAGWGIAKIQINDNPVKWF
                     SKRHPIRIADIALNRHFGGTYMAYLILDGQPPQKATTEDLEYIRKGLAAFAAQIQQDY
                     ANAPILAEKIQIQLDSLFAASDSYKGFLDAVVRWIDTQAKNAPDEEYYTWDELRVYFG
                     IEGERLKVFKRPDVLNYMAALQSHLEQTGLVGKSNSVADIVRKINQELVDGRPENYRI
                     PEQLQGVAECYVQFQQSHRPKDLWHFLTPDYLHANIWMQLTSGDNKDMEQVVRAVEDY
                     FAAHKPPVELTYHWAGLTYINTVWQEKMVFGMLQSFLGSFLVVFIMMAVLFRSPLWGL
                     LCMVPLTITIAAIYGTIGFIGKDYDMPVAVLSALTLGMAVDFAIHFLQRSRETHQAAG
                     SWAKAAPQMFGEPARAISRNILVIAIGFLPLLAAPLVPYKTVGIFLCAIMAVSGFITL
                     AALPAVLTVAEKYFFKAGRKIESVGCNCSFCIIIAVSVAALVALNLHQYWQVGFTKLT
                     GMSIALIPVLVLVCGLMSRRKACQTNRDNQISREDGGVK"
     gene            27482..27673
                     /locus_tag="PLE88_03025"
     CDS             27482..27673
                     /locus_tag="PLE88_03025"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOM75471.1"
                     /translation="MTVEKALRAIAGGFVLTSVLLASFHSIHWLWFTGFVGANLLQSA
                     FTNWCPMIVLLKKLGLPSS"
     gene            27698..28525
                     /locus_tag="PLE88_03030"
     CDS             27698..28525
                     /locus_tag="PLE88_03030"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOM75472.1"
                     /translation="MKKLIFLLVMTAVLGLRTFAQESAAPTVEEIVKKANHTAYYQGF
                     DGKSRVVMNIIDKQERTRSREFVILRKNTGQTDGDQKYFVYFQSPADVRKMVFMVHKH
                     ASVDQEDDRWLYLPNLDLVKRIAAGDKRTSFVGSDFLYEDVSGRNLAEDTHLLVQTTD
                     QYYVVKNVPIKPETVEFSSYTVFIDKKTYMPMKMEFYDKDNVLYRTIESIKVEQIQAK
                     EADQEVTYPTVTQSVVKDLKTGSRTEMTFSDIQYNIGLSDDIFTERYLRRPPREAMR"
     gene            28538..29812
                     /locus_tag="PLE88_03035"
     CDS             28538..29812
                     /locus_tag="PLE88_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75473.1"
                     /translation="MKVKWIWMTVIFFSIGAGAVAEENDGASPWQQLFSSVDVHGFTE
                     LRGGYRIRKDPYQKDMSVMEARTQLDLSSYNDWGDFKYKGDIWGDLVAEQGDYETREL
                     WFFTRPADWMDFKLGRQVLTWGTGDLLFLNDLFPKDWQSFFIGRDTEYLKAPSDAAKF
                     SFFTDAANIDVVYTPKFDSDRYITGQYLSYWNNSLSRRAGRDAYVYTDKPDRCFRDDE
                     IALRIYKNINNYEYALYGYWGFWKSPAGMTPTGSATFPDLNVYGASVRGQVAKGIGNA
                     EIAYYESADDQNGKNPLISNSELRYLVGYTQEIAKDVTGGLQYYLEQMLDYNAYLRHL
                     SGGPKRDRDRHVLTARLTKLLMNQNLSLSMFTYYSPSDSDVYLRPNIHYKATDTLSVE
                     TGANIFFGDHPHTFFSQFENNSNIYAAVRYSF"
     gene            30151..30630
                     /locus_tag="PLE88_03040"
     CDS             30151..30630
                     /locus_tag="PLE88_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75474.1"
                     /translation="MLLLTGEYEHTIDEKNRLFISNKLRSQIDIRKYGCNFYLTMGSN
                     GILCLYPDEYFEQIALAGAPRTGAPDEAVAFERLSFALASKVELDRQGRLLINEKLRK
                     RANLGTALTLVGVRDHIEIWNTEDWEHYLEDNLSQFHTQVAQARQAQLQRQGQDLER"
     gene            30643..30852
                     /locus_tag="PLE88_03045"
     CDS             30643..30852
                     /locus_tag="PLE88_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75475.1"
                     /translation="MKTQTNNPVEVLQQGLTGRRPVDEQVLSCAALLAERLQQLKSAD
                     PIFDSIAFSPDVEAMMASHLTAVVN"
     gene            30881..31807
                     /gene="rsmH"
                     /locus_tag="PLE88_03050"
     CDS             30881..31807
                     /gene="rsmH"
                     /locus_tag="PLE88_03050"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOM75476.1"
                     /translation="MSVHQDKGIDEPLPAEHIPVLLKPLLDIVQFPTDGIMVDATVGH
                     GGHSRQFGRRLGPEGLLVGLDVDAECIRRARSSLSPLACRVLLLRENFADISTVLRQQ
                     GIDKADFILADLGFCSAQMDDVQRGLSFQKPMPLDMRLDDRLTTTAADLVNQLSQTEL
                     ADVIYRYGQDRASRRIARFIVQQRSVRKLTTTTELAELVCRALKQRPHAGRIHPATRT
                     FQALRIAVNRELENLDRLLETAPALLKPGGWIAVISFHSLEDGRVKNNFRKNHADGRY
                     EIITKKPIVADAQEVAQNPRARSAKLRIARCL"
     gene            31830..32696
                     /locus_tag="PLE88_03055"
     CDS             31830..32696
                     /locus_tag="PLE88_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOM75477.1"
                     /translation="MTADAKIGLLLGLFFIVIIAFLVNGLPNFVRQNDSKPLDAAITA
                     SGSSELIIDNSLTEAIHRLRPQIELRQTEPSNEVLVIDSSAQPIPQQSLETVSQPQMK
                     VQTEVAAAVTPVPAPKQESGIQAVQPASRSVQPVSQKFKTHIVQSGEHLSSIAQKYYG
                     KEDGNRRLVIQKLYETNAKTLKSPDHIQAGDKLVIPPLDELLGAPQPSVSQPSETLLS
                     KFSSLLERAGKEDTKKVSEYVVRQGDSLWSISEKTLGDGKRYNEIIQLNKDRIKNADD
                     VAAGMTLKIPAR"
     gene            32707..32949
                     /locus_tag="PLE88_03060"
     CDS             32707..32949
                     /locus_tag="PLE88_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75478.1"
                     /translation="MSRLRVIFVVFSITACLVFTIFLRTSASRMFNQYRSAEVEQKNL
                     RQQLWQKQLRFEYLVNPAGLPPLPPPDDSAKKGGMQ"
     gene            32946..34697
                     /locus_tag="PLE88_03065"
     CDS             32946..34697
                     /locus_tag="PLE88_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOM75479.1"
                     /translation="MTYSRRERWLLGLFLVLILPAFGGLIWRLFDLQYYQKETFDKVS
                     RRQQNAVVPDKPRRGLILDSRGRVLAASVKTWNAFAEPRRLREYPDQIQVAASALRDI
                     LNMPGYEICRIIEESRNPGYVKLKAQLTPGQRDLLSHCRLAGIGVEADWIRQYPAGSL
                     TSHLLGFVGAENTGLSGLELQYENILAGQPGQQVFIVDAQRRPIGTQMQEAIEARDGL
                     NLVLTIDSVIQHFTREALLKKMTEYQAESAVAMVMDPWTGAVLAMVSLPDYDPAAFST
                     TPQDRMRNRILTDSYEPGSIFKPIVAAIALDAGSVGFNEKFDCENGYWPRYRIGEFGN
                     HQYGIFSVREILIHSSNIGMAKIGLKMGQKELYKGLKLFGFSEQTGIDLPGEEPGLLR
                     PLSQWSGWSVTRIPFGHEISVTGVQICRAYSILANGGYMIKPHIVRAVVDNQGTVIEN
                     KQPPVRTGYILKSEVAQWIVRNALVGVVNEGTGDQAKMEQIQVWGKTGTANIALSGGG
                     YDTRNYVASFVGGAPADKPAVVVLVSIRKPNRALGKGYSGGRVAAPVVKEILENTLTY
                     LGIINSQQLPVRQVVQQ"
     gene            complement(34654..36780)
                     /locus_tag="PLE88_03070"
     CDS             complement(34654..36780)
                     /locus_tag="PLE88_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOM75480.1"
                     /translation="MYHFRLKIFILLCLAGLTIAVSRLMILQICHVQQAREEVEKLRI
                     LVPQQRPTIRGRILDRSGNPVALDKPAFFLHINYELTRLMDARWREGRILRAVTNSKS
                     RQEIEREFYEEKWKEPLETLNRTIELALQLADASREEITANIQQINDFIWERARYIFW
                     LRRNQGRSRADYLAQRELIRPEDIVEIDLWEMHQSYPLIELKTQNDLLRAQVELLGIQ
                     ELEIKTEAKRYYPYGAAACQIIGWVAPWQEHEIEQFEHDKYMSYQEGEVIGKSGIEKI
                     YESVLRGRRGEVTYDRDGNLLERIEPQYGRDVQLTIDMDLQQKIEKLLADKRMPHEGK
                     PSAAVVLEAANNDILAIASIPVFDLNTIRQSDNYNRIFNPRDPNKLWENKALERNYPP
                     GSTAKPLVLIAGLEEHKISPTEPISCSGWHLPPEGWPRCLLQRNYGIGHDDHFAGEGG
                     NIARNAIRGSCNVYFSQLANRLKGQALQSWLFQFGLGQDVLLTPLPSEAAGNNTEDIL
                     LNQSRGCLIYDVYQPKVQNASELKPIPDWEKKWWGMGQGSLRVTVLQTANALSAIARG
                     GIYKAPRLVFDDRDPHNERYQRRIPVSPSTLSVVRDGMKAVVYESHGTAREEFRNSEL
                     FSRGITIYGKTGSTERPYHAWFECFAEDKTGRTIVVAVLVEGGQRGAGEAAPLGHRIL
                     YYCNEAGYIGEKPTAAPPAGPEAAEN"
     gene            complement(36783..37289)
                     /gene="mreD"
                     /locus_tag="PLE88_03075"
     CDS             complement(36783..37289)
                     /gene="mreD"
                     /locus_tag="PLE88_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOM75481.1"
                     /translation="MRWPTFFVIVFACTLLEAGNLLTVFSTGGWYIRPSLLITVLVYY
                     ALSTRTRQAIVCSFLIGFAADLAAGLIGPHTVCCGGLGLLLNQVRQLLTVRQALYRAG
                     IIFAVYLTAETFTLWLGMLKVRQTIPYSTVLLTGLYSAVISPLLWSFLSILNRGAGTR
                     ESLSGLNE"
     gene            complement(37298..38134)
                     /gene="mreC"
                     /locus_tag="PLE88_03080"
     CDS             complement(37298..38134)
                     /gene="mreC"
                     /locus_tag="PLE88_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOM75482.1"
                     /translation="MAGKHIHFSNTSLFFSFLTAGLILLLLPHQTTSKISLLFYSAFE
                     KVLRLGRDVQLEALRLHPGQEENVSRYEYEKLWKNYKNLHAQLMQLHRDYEALAYLRT
                     ALPLPGSGLVLAAVTGAASPYSHEIIINKGSTAMIRPGQYVLSEQTHCVVGIVEETSE
                     HAARVRLLTDANQSIEVRIRREGTDKDIGAILFGSSKGLCRISMIDRQQDVREGDTVY
                     AASRPGKLEVPMVIGEVISVQPDELHPLLWDITVMPAEDMTRLDSVAVIVADEMLLLR
                     KE"
     gene            complement(38209..39255)
                     /locus_tag="PLE88_03085"
     CDS             complement(38209..39255)
                     /locus_tag="PLE88_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOM75483.1"
                     /translation="MILDTILGWFSKDMGIDLGTCNTLVCVRNEGIVLNEPSVVAVRR
                     GTNQVLNNGEAVGLVAKEMLGKTPGSITAIRPLKDGVISDFEITEAMLSYFIRKVHGR
                     GGLFRPRVVIAVPSGITAVERRAVIDSAERAGARKVFLVDEPMAAGIGAGLPVTEPTA
                     SMIVDIGGGTTEVAIISLSDISTCESIRIGGDNFDEALINHLKRTYNLLIGEPRAEMV
                     KIQIGSAAPLEQEMTMEIAGRDTISGLPRKIVITSEEIREAFKEPIAGIIETVMRTLE
                     RAEPELAADLIDNGVTICGGGSLLRGMDKVLSNATGLKVVHAEDPLTCVARGTSVYLE
                     NLEIWKDTLDQGWS"
     gene            complement(39528..40382)
                     /locus_tag="PLE88_03090"
     CDS             complement(39528..40382)
                     /locus_tag="PLE88_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75484.1"
                     /translation="MSILTKILAVLLSIFSILLCGMVVTFVGNSANYKGWYEEQKSMN
                     IVLQGELATKNEQYDTLTKKCSELELKLQQDIQNLEAEKNQLAADLRKAERLAQQYQA
                     QADSWKGLLTGFEQSIRNLQATLSQTQQQLDQARAQGIKDQKELNQITAYLHEKIVQL
                     QSMEAERRRLLEQKQELENQISSVGIGIREAIPVTPLAKTAAFFVKQKTAYEIKGLVT
                     EVQENLITLSVGSADGVKEKMVFHITRGDRFLCDVIITDVDINQSAGVLDLVQQRPQV
                     GDTASTQL"
     gene            complement(40568..41557)
                     /locus_tag="PLE88_03095"
     CDS             complement(40568..41557)
                     /locus_tag="PLE88_03095"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM75485.1"
                     /translation="MKTVLLFSLPLVLAFAGCTNSAPPAPQPAAGLETPAKLQSLKAR
                     ALVILEAALNSDNAYLRSHAIEVVAETRQKQMLPLVVKLTTDRSVAVRFAAATGLGDM
                     QCFSCENVLTRMLNDPDMNVQIGAAYALAKMNQLKYRELIRTAAVSTDPTVRANAVLL
                     LGKLGNRDDLALLYEVLNNNESTDKVRMQAVESIARLGDERMYRSKLWALLISKFADD
                     RVMGIRGMGALNTPESRGAIVTMLQDDVPEVRLTAAEQLARLGDKRGETQVYEYFQTQ
                     PDLNKMDMANGMAVRAIGYLASSRLNSLLPQIIETPSRDLQLIAAQAVLLQAK"
     gene            complement(41560..43248)
                     /gene="lnt"
                     /locus_tag="PLE88_03100"
     CDS             complement(41560..43248)
                     /gene="lnt"
                     /locus_tag="PLE88_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOM75486.1"
                     /translation="MGQQKINHPLFNAVLGFAASGILLTVIQAPWNLHFLAWLAWVPF
                     MLACKSKLQTGRLLLSAYFIGLCYWVGNLYWLYIITIAGYLAFAVMQGLYWPVLAWLV
                     RFAQRKNWPLALMAPVIFVGAEAWQGILYTGFCWYYLAHSQYRNLSIIQICDIFGALG
                     LSALIALVNGVIAQWIIDRRSGRTAAMRTFWSAAAAVLLIMLTLAYGQHRIRQTPAYL
                     SRGPLVGMVQPNVPAWIKEEMDNGQKILDDLIADSNECIKAGAVMAAWPETMVLAPLN
                     QEYLDLCVANSDPPRFHRQIIEHCKDRAYILAGAHGAAIGIQNGRYAITDQYNSAYLY
                     RPDGTADPQRYDKIHLVPFGEYIPFRDTIPWLYKAILWLSPYDYDYNLTPGKEFTTFE
                     IKDNSRVYRFGVLICYEDTDPTVTRKLAVGPDGKKKADWLVNLSNDGWYVRFKNGRVY
                     PMAELAQRTAISVFRCVENRISIVRSVNTGISCLIEPTGRIRNGFLAGTLPAEAMDRQ
                     GVRGWFVDRVPIDSRITFFSRHGRWLDVVFGAGWSIILIWSIYDVCRKHLRKDK"
     gene            complement(43230..44198)
                     /locus_tag="PLE88_03105"
     CDS             complement(43230..44198)
                     /locus_tag="PLE88_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOM75487.1"
                     /translation="MQKLHNTIICGDCITVLNGVTEPFADLVFADPPFNIGYPYDKYH
                     DKVETNNYLAWTRDWMAACVHVLKPHGSFYIAIGDDYAAHVRIIGDQLGLHCRNWIIW
                     HYTFGQQTKNKFARSHTHILYFVNDPKNFIFNDLAVRLPSDRQLIYNDRRSNAAGKIP
                     NDVWDSYSRVCGTFRERQGWHPCQMPELLLGRIIAASSRTGDLVLDPFNGSGTTAAAA
                     FQLGRQYCGIDISEDYVQKTCRRLADIEKTEHSYQNGQLSTTEFFEFKRLFAEMGIAA
                     ETLIHNSKMLTLFMNQLRLRLNSPTMYDVQLIKEALKDLSTWASKK"
     gene            complement(44210..46180)
                     /locus_tag="PLE88_03110"
     CDS             complement(44210..46180)
                     /locus_tag="PLE88_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOM75488.1"
                     /translation="MGHLTRLAVGLTLLISAVSVHAGTAEIKSPDGKLKVIFTDSPML
                     GYSLAYDGTVLMQNCPIGLTIRTADGQLTELGKNVVLPESSQTLVDETITPVVPEKKR
                     IIQDQYTTVTFPVNSSFSLICRVYNDGFAYRIQTKLPGEVEVVAEQIAFAFSEDYPIF
                     FPQEDSFYTHQERSYINKPLSQLTKGAFCSIPAVVDIPGGPKIALTESDVIDYPGCYL
                     QAQGTTTLKAIFPNYPLEERPQGDRDVRVVKRADYLAKTSGTRLYPWRVVIVAPTDAD
                     LVLNQTVYKLASDCTLEDTSWIRPGKVAWDWWNANNVYGVDFRAGVNTATYKYFIDFA
                     SQYGIEYIILDEGWYPLGNLLKTVPDIDMPELLRYAKQKNVGIILWTTWKTLDEQFEP
                     AMELFSKWQVAGLKVDFMQRDDQKMVQYYEKVAAEAAKRHLLVDFHGAYKPDGLRRRY
                     PNVITREGVQGNEHSKWSKNITPSHTVTLPFIRMVAGPMDFTPGAMRNFQPDQFNPVF
                     NRPGVIGTRCRQLAMYVIYESPLQMLCDSPSNYLREPECMEFLKPVPTVWDDTRVMAA
                     KIAKYLYMARKKGNEWYLGGMTNEDKRTAVFDLSFLDEGTYAMTLFQDGINADRFAED
                     YRKITKTVTKNDTLTIELASGGGFAARLVKQQ"
     gene            46373..47116
                     /gene="kdsB"
                     /locus_tag="PLE88_03115"
     CDS             46373..47116
                     /gene="kdsB"
                     /locus_tag="PLE88_03115"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOM75489.1"
                     /translation="MKIIAVIPARYASSRFPGKVLAKETGKYLIQHTYERALCAKCIS
                     QVLVAADDQRVADACRQFGAPCVMTSVSHPSGTDRIAEAAAGVDADIVVNLQADEPEI
                     APAYIDRVAMLLVEHPQAAMATLLAPFESIADIANPNIVKCITDNSGRAIYFSRSVIP
                     YDRAAGGIGRIDAYHRHLGIYAYRKEFLMTFTALQPGFLEQHEKLEQLRALENGYAIL
                     TACVEKTWDGIDTPEQYRAFVERIRQITQ"
     gene            47132..48901
                     /locus_tag="PLE88_03120"
     CDS             47132..48901
                     /locus_tag="PLE88_03120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOM75490.1"
                     /translation="MSDSMDLLAKISGTSTDNEYFLPLPPGYQKGKTRYVIVMGTVMS
                     GLGKGIFSSCLAKLLQDKGLTVAPIKLEGYLNIDSGTLNPFRHGEVFVLDDGMECDMD
                     LGTYERMLDQNLSRQNFSTSGQIYSSVLEKERHGDYLGRDVQMIPHVTGEVKMKLRQL
                     AMKTNADVVFVEIGGTVGDLENAFYIEAMRELAYEEGPNSCCFVALTYILEPKALGEQ
                     KSKAAQLGIKQLMQMGIQPDIIACRAEKPVSDQARQKMSVFGNVPFERVFSMHDSASI
                     YAIPSMLRDCGIDFEVLRMLKIEDRIQLRHERKEWARWCDFTDKIGKEAYDITIGITG
                     KYTSVRDSYASVLHAVEHAGIHLGIRVNIKWIETTDLTDDNAAACLADVDGIIVPGGF
                     GTRGWEGKIACVHYARQNNVPYLGLCLGFQAAVIEFARNVCGLAEANSTEIDPHIGQP
                     VISILPEQKKIEGLGGNMRLGGRDVEIKKDTMAWKLFGKTSIVRLRFRHRYEVDPAYV
                     GLLEEKGLVFSGKAPNQPIMQILELPSQLFFMGTQAHPELTSRPMRPSPFFVGLVAAA
                     GKRRYPNAGLDNPLEAVEKVRGF"
     gene            48988..49299
                     /locus_tag="PLE88_03125"
     CDS             48988..49299
                     /locus_tag="PLE88_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75491.1"
                     /translation="MKYGMAAVVLCVAVFAGYCTSAWSKASVPVLYIVGDSTAANGPD
                     KGWGSHLQKFFDPNQLNVVNAARAGRSSRTYITEGFWDDVVKNLKGTSNNLIFEDQNL
                     S"
BASE COUNT        12189 a        11953 c        12729 g        12435 t
ORIGIN      
        1 ggtttcccat aggcattggg gatgatatgg cacaggtccg ggtgggtgaa gatggaactg
       61 tccttatcac aacggatatg ctgctggatg gtgttcattt tgacttgtgc cgctgctccc
      121 tgcgccaagc cggctataag gcaatggctt gcagcttaag cgattgtgcc gcgatggcaa
      181 cggtgccgct ttgtgcagtg gcagcggcag cattgccgcc tgattttgga caggccgaat
      241 taaaggaact gtacacggga ttaacacaag cgggacgcgc gtatgactgc gaactgattg
      301 gcggagacat caccaagtgg cggagcaaag aaagccgatt tgcgattaat gtagccatga
      361 taagcagacc cagcggcttt catccgccgg tgcggcgtaa tggggcaaaa ccgggtgatt
      421 atatctgtgt tacgggttcg ctgggggggt ccttagccgg caaacatctg tattttaccc
      481 ctcgtgttaa tgaggccctt cagattactg aaaaggcagt tatccattct atgatggata
      541 taacggatgg attaagcaca gatttgaacc gcatctgcaa gcaaagcagg gtgggtgcaa
      601 ttgttgaggc agcaaatatt cctgtttcca aagcggctca aaagacctcc aatcctctgg
      661 aggccgccct gcatgacggg gaagatttcg aactgctttt taccttagcg cctgaagaat
      721 ttcagaaact cccctctata ccggcagttc ctattgtcaa aataggcaca atcacggact
      781 cgggccggtt ggaaatgaaa tatgccgatg gaaaggttgt tgtgttaacc ccaagagggt
      841 acgatcactt ataacgcttt gcatgatgtc aaaaggcccc atcgagttta taacacacaa
      901 tgccgccgaa acgattgcat tcggacgccg aatcggcgcc ggattgcgag gcggtgagat
      961 tatcgccctt gtcggagacc tcggaacagg caagacccat ttggtaaaag gcattgtcct
     1021 tggcttggat gcctcagatg caggggaagt gaccagcccg acattcgttc ttgttcatga
     1081 gtatcttgga tgcggcgggg cgaaaaccat ctatcatatc gatgcctatc ggattgaatc
     1141 gacggctgaa tttgaggcgc tgggatttga tgaatattgc agacctgata cgattgtgct
     1201 ggtcgaatgg gccgataagg ttctatcggc attaaacgga tatgatgtta tccaattgcg
     1261 gctggagcat atcggcaaaa gcaaacgaag aatttctgtt tacaatccgc cggattttct
     1321 ctcggcaaaa taataacgaa tcgggcttga agctattctg cttggagggg gcactctctt
     1381 cgagatttac ctgctaaatt cttaaattgt cgaatgattt ttgtctctga acagattttt
     1441 tgcaggctgc tttgctgttg gtttacaatc cattattcaa tccccaagat tgctgcataa
     1501 tgggcctgtt tatcggttct tctttatatt ttaggctgtg tgcaatcgtt atgaatattt
     1561 tttgattttt ttgccctgca tagttgccac ttgtcctttt tatttattaa gttttctttg
     1621 gataaagttg gaagttcttt gacaagcgaa tatcggcctg gcgacctatt gagagatagc
     1681 agagcacaga gagtgcctca agtatccgct cctacgtgtg gaagcaaacc gttaacgaaa
     1741 cgcaaaaaca ataatgtatt tcagacaagc acaaacaaag aaagggtacg aaaatgagaa
     1801 agaacaaagg ttttacactg gttgaaattc tgattgttgt ggtcattctg ggtatcttgg
     1861 cagcgattgt gatacctcag ttcagcgatg ccagcacaca gtcgaaggtc agcagcgcca
     1921 agagcagcct gcagaccctg cggtcacaga tttccctgta taagattcag cacaacgata
     1981 atcctcctgc tttagccacc tttgccgctc aaatgacggg caaaacagat attgatggtg
     2041 cggccggaac acagtatggc ccctatatgc aggcggttcc caagaatccc ttcaatgaca
     2101 gcgacacact ggctgctgca gatggtgcca ctgtcggctg ggtctatgac gagacaaccg
     2161 ggattatcaa actgggcggt caaggcgcca cagacctgaa tggtgatggt gatacagctg
     2221 atgcaggtga aacccatatt gactggtaag tcttaagatg tttctaacag ccggccggct
     2281 cctggttttc cggggggcag gccggctttc tttgttaata cagggaattg ccaagaagac
     2341 ggtaagcaaa ttttgaatgg ctggaaaaat tgtcaggacg agttcgctga agatgggaaa
     2401 ggaccgaaca atgaaagccg gtaaggaaaa tccaagaaaa ctaacaaagc tggatcggtg
     2461 tgttctgatt ggattggttg ggacagccgt ttttctgctt gtgccacctt tgattcaggc
     2521 cggacaggaa aaaaaactgt caaaaatggt cgatcgttta cagataatcc gctcccagat
     2581 ctccctgtat aaagccgacc ataatggtct tctgcccggt caaagtgctc ccggccagag
     2641 catctgtccg gaagatattc tgttggcaat gatgcagtcc cgtcccaatg gcagcgggcc
     2701 ttatctgcgg cagattccgg aaaatcctta tttggcggaa accggcagag gcacaatgat
     2761 tacttgtgta aatgatccga atgcccagcc aacgggtgca gaaggcacgg gttggtggtt
     2821 taatgctgcc acaggacagt ggaaggcctg tgattcagaa tttcatgcaa attattaggc
     2881 ttgtgtcact taaattatcg ttttttcctt aaccgcagag acaccggtag atagttattt
     2941 acgatgatgt acggcaaaac caaaggtttt acgctattgg aagcaaccat ggcgatggtt
     3001 attgtggcca ttgctgcggc gggagtcttg ctgccgtttg cccaagcagc atcggtccat
     3061 gccgaggcgg ctcgacaaac aatggctgcc aatctggctt cggaaatgat ggagataata
     3121 gcggccagcc cttatgatac tattatggat tataacggtc agatagaaaa tcccggtcaa
     3181 ctgaaggatt cagagggcaa ttatcatacc ggatcggcct acgagggatt tggccgttcg
     3241 gtgttgtgtc agcacgttgg gtctgtaaat cttgccaaag tgaccgttgt ggttacatac
     3301 cggaatgctc ctgtaacgcg tataacaaca ttgatcggca atcacagata atccgttgtg
     3361 tcaatgagat tactcgaatg aaaagacgta tgcatcaata tgggtttact ttagtagagc
     3421 tgattattgc actgatgatt gcctcggtgg ttatggccgc ggtagtaacg ctggctaatg
     3481 cagcagtcaa tgccaatcag gccactgatc tgatggggcg tgagcaggca cagcttcggc
     3541 tggtgttgat acgtttgaca gatttgatac ggcgagccaa tcaggtgacc gctgcggata
     3601 ttgatagttt ccagctttgg catgataaca atgctgatgg actgaaaaca gcggatgaac
     3661 tgactcaaat atcccgggga accggcggca atacacttca aatcgacagc atcgacagca
     3721 gcgaggtgta cacccaatgc cgaaacatca gttttgacta cgatagtgcg gcacctcaaa
     3781 cccgcaaggt aactatttgg tttgatataa cggctgccgg ccaaagtcag cgttatagta
     3841 tcgtcgcccg cctgcgggct tcagacgaac atcagaaact ataacgggtc tgcttcgaaa
     3901 aatatggcgt tttttgttca gaatggatga gatgaaaagc tataagcgaa gatttagttc
     3961 acaacggggc atggctatgc tggctgtgct ctttgtggtg ataatgatag cgattgttac
     4021 ttcggatttt atcgcacgct ctgatgcatc actggcttca ggcaggaatt tatgtatccg
     4081 caacgaggtt gactatgccg cttggggcgc tttggaagcc gcttgggctt tgatgcagga
     4141 tcccaatgtg cgcgctgcat tgccttattc ggctgttgcc cagcagcttg atgatacatc
     4201 acagatctac tatgagctgt ctattgagtg ctgcggggca gatcccaatt tgtatccggt
     4261 tgaatgccgt gcctataagc gtgttaatgc cgacataaaa gcccggagtg tccagagtgt
     4321 cctgaaagct gaccttaagg ttgatcccaa cagtatgcaa atctggtacg cttcaatccg
     4381 ccggcagtaa tgtccgataa tctttggctg ttttggaagg gcattgcagc atagccccat
     4441 tatgtcattt tgcaggcgga atctggagat tcgtcttttt agcgattaag cgaggttgcc
     4501 aaggtgacga caatacatcg ggattaatgt cggctcccag cagaaccaga aaaaacgagt
     4561 atgaaaaagg caataacaca acaaaacagg ctgacagcag cgggatttac gcttattgag
     4621 ctgattctta taatggtgat tatcggaatt gcagctctga tagccattcc cacaatatcc
     4681 agcgcggcgg atgtgcaggt tcgcagtgct gccaaccgtc tggcagccga cttagattat
     4741 gccaagggat tggccattac gcatcagcgt ctttgtgcgg tcgtttttta tcccaacagc
     4801 gagtcgtatg atatccgctg gacagacacg gatgaaattg tagagcatcc aacgcagccc
     4861 ggcagaaact ttgtggtcaa ttttgtctca gagggcattc tgaaacgcgt caatctggac
     4921 agcacgaact tggactccaa tacaattact tttgactatc tgggaagtcc ttataacgga
     4981 aagggaacag gtcagccgct caacagcggc cgtattaccc tgaaagcgga tgcatttacc
     5041 ctgtatgtag atgtagagcc ggttaccggt tatgtaacga ttaacgattc ctgattgctg
     5101 gcagaagtgc gggttcaaca gatgcttcaa aactggctaa aaccgaagtc gcttccgatt
     5161 ggtctggatg ttggacattc catgatcaag atgattcagc tttgctgcaa agatggttac
     5221 atcagtgtcg aagcagccga agaagaaatg ctggatcgga cgcttgctcc ggattccgat
     5281 gaatggcgcc ggcatacggc tgatttgata accgcaatgt accggcgggg cggattccgc
     5341 ggccgaaagg tggtatcctg tctgcccggg gatgcagtga aaatcaaaag tctgcggctt
     5401 gatacggttg aacctgaaga aattgaaaaa attatgcata cggaagtatc ccggcgtttc
     5461 ggacttgacc cccaacgcga tgagatacgc tatatggtgg ccggcaatgc ctatcaaggc
     5521 gaagaaatta aaaatgaggt tatcttcttc ggcattgaaa gccggcaggt aactaaccat
     5581 ataatgctgt tggaagaagc agaccttgtt cctatctcgc tggatacagt tccctgtgct
     5641 ttattccgct gctttcaggc aactctgcgg cggcgggaag atcaggattt ggtcagcgtt
     5701 tttgtggact tgggttcaca atatacaacg gtgattatcg gcaaagggca ggaaattgcc
     5761 tttgtcaagc agataccgat tgccggcaat gccttaaacg aacaggtcgc attgcggctg
     5821 gggatctcat tggaagaagc ggctgctgtg cgggctcgtc ttcgcagttc agatgatcag
     5881 cagaaaaatc ccgaaacgag gcgagcggtt attgatgcaa tgagcacctc cattgaaatg
     5941 ctggctcacg aaatttccct gtgcttccgc tattatgctg taacctttcg cggacgccgg
     6001 ccggctgaag cagcgtttgc cggaggcgag gcctatgaat ccgctctgat tgatgccctg
     6061 cagcggcatt taggggtaga gattcggatt gccgaacctc tgcgcggtta tgatctaagc
     6121 cgtgctaagt ttgaccgccg gcgcaatcct cagatgtgtg aatgggccat cgcagtcggg
     6181 ctggccctga aggggatgga attgaatcaa tcacggcagt atgcagcgca ggagacggaa
     6241 gtgtctgtat gaaagaaatt gatttcatcc cggaatggta caaagccgat cgccggcgca
     6301 ggcagaggta tattcaacag cgtactctgc tgggcattat atttgccgtt atggtggttt
     6361 ggagttttgt tatgggccag catgtcaagc gggtacaagc cgagatgaac agtgttcaga
     6421 ctgctttcga gctgggtaaa gcacgggttg aacaagtcca tgaattggaa cagcagattg
     6481 ctgtaatgga acaaaagacg cagctgcttg ggtatattgc tccgcgaaca aaaatttcgg
     6541 ctctgatggg agagatttcc tatctggcag gcagcaatat ccttctaagc cggctgtgct
     6601 tccagaacga gccgctgcag tctccaaaat ctgttgtcca gcattcagca gctgtggtgc
     6661 agactgcggc cgccgcgagc agccaatcct gggagcgtca gcaggtgccg ctgcggataa
     6721 aaacaacgct gactggaatt gctgcttcgg gtgcggatgc agccggactg atatcttctc
     6781 ttgagaagtc tgattatttt gagcaagttg tccccgtttt tacccgggca aaaaaggtgg
     6841 gggatactga agtaattgag tttgaaatcc gatgttatgt ggcggattac aggatacagc
     6901 agtaaaaaaa cgatctatga aaaatctgga taaacagcaa acagccatac tgattctggc
     6961 cgcaatagtg attgccggct ttgcgggctt tcgctatgtc ccccttgtcc ggcagaaata
     7021 cgcccttgac gtgcaggtca aaatgcagaa acaaacaatc gaacagattg cggcatccag
     7081 tgcattgctg ccggaactca aacagcagca taaactcatg accgagcggc tggaagcatt
     7141 cggccggaag attcctgaag gacgcaattt tgcaaattta tggcagcaaa ttgcggatat
     7201 gatgaatgca tgccggctga cagatcaggt tgttcagccc ggggctgaat taacctccga
     7261 gactgtgtgc tgcatccctt tgaccatcga atgcaaaggc accgcagaac agatgtttgc
     7321 atttttccag tctctggaaa atatggatcg tctgatacgg atggatgaag tccggtttga
     7381 aaatgacggc aattacagcg gaatagttaa actgacagcc aaggccagcg tgtactatca
     7441 gccttcggct gtccgcagcg gctgaaatac tatgaatcga aaaacaccca gtcttgcaag
     7501 aaaaatacag actcccgcca gtctgcagaa catcagtccc aagatgatgg taatggttgt
     7561 tttgctggct gtgatgggaa tattgtgggg acgcgtactg ctgggcggta aaaaaggtcc
     7621 tgccgtcgct gaagctcagc aagcagccgt atctgccgcc gaattacaaa gcgttcagtc
     7681 tgccccggcc attcgcatca atcctgtgga attgccttgc cgactcggtc gaaatgacca
     7741 gttaacaaat gatcttttca gtcggaaggg ctttttatcg gctggtgccc aaagttcgtc
     7801 aagggacagc actgcagata agcagtcgcg gctggagcgg ctggcgaccg ctcagctgac
     7861 gcttgaagcg gtggtgcggc gggaggacgg aacgccttat caggcatttg ttaacggaaa
     7921 aatcgtgacg gttggtgcaa tgctgacggt caacgaggga cctgaccaat atgtattaac
     7981 agtgacggat attaaagaaa acagagtaga actcgagtgg aacacagttt cggttgtttt
     8041 gaaaatgacc ggaaccagca atcagtagaa aggatggtgt tttatgttgg caaaacagaa
     8101 tcgaaacgca ggaaaaacaa agcttgctgc ggcaggagtg tggatcttga cggcagcagt
     8161 gtttgctgcg ctggcggctg cggaagaacc ggctgctgct ccggaagaag cggctgttgt
     8221 tcagcagggt gtgatgggcc agtcaattca gacaattact tttaaaaagg atatgcccat
     8281 tcgcgatgcg ctggcaatgc tggcgcagat gtatcagaaa aacattgtgc catcggctgc
     8341 agtggacggc atagttactg tcagcaatct gtacgatgtg acatttgagg aagcccttca
     8401 ggcgatactg ggcacgcata agtatgaaat caaaggcaac ttcatcaagg tttacaccaa
     8461 ccaggagttt atgcaggata aaacccgctt tgagcatgca attatagccc tgcattatat
     8521 caactccgaa gaagccagaa agctggccga acctctgttg agtgaattcg gcaaactggg
     8581 cgttaccaca gcggcacctg ccgaaatgca ggccggcaaa ggcggggata cgttggccgt
     8641 gcatgatcgg ctggtggttt cagattatcc cgaaaatatc cagaaaatcc gggacattct
     8701 ggcagaagtc gatgttgaac ctcttcaagt gttgctcgaa gtaacggtga tggaagcgac
     8761 gctgacggat gataccaagt ttggtctgga ttggaaaaac atcagcgaca cctctatcag
     8821 tctgggcggc aaaggattta ttcagagtgg ttttacccct tccacatccg ataccggcgg
     8881 attaagtgtg ggtgtaacct ttgataatat ttcggcgctg attacagcga ttgaaacggt
     8941 ctcggatata actattatgg ccaatcctaa gatattggct cttaacaagc agaccggcaa
     9001 gattatcatt ggtaatcaag acggctacct gtcgttaacc aatgttgctg aaggcgggac
     9061 gtcaacccag caggttgaat ttctcgaaag cggtacagtt cttgaatacc gtcctttcat
     9121 cggccgcgac ggaatgattc gaatggaaat tcatcctgag caaagcagcg gtgaaataaa
     9181 ggtaattggc ggtgcaacac tgcctaaaaa atccaccaca gaagttatca ccaatgtaat
     9241 ggttcaggat ggccagacga tagtgctcgg cggtctgttt aaggaacaaa ccacacaatc
     9301 gcattcgcaa gtgcctgtgc tgggtgatat tcctgtggtg ggtgaattgt tccggggcat
     9361 caatgacaaa tctgaacggg tcgagctgat agtcctgatt acccctcaca tcatcaacac
     9421 gcctaaggaa gccgatggag ctgaccggct tgaagatgtg atgcgtctgg ctcataacgc
     9481 gcgccagaat ctttactgga tgagccgtgc caagatagag gaagaccggt atgccaaagc
     9541 cgtcaagctt tatgccgaag gccagcccga agcggctctg gctatgcttg atcatcctta
     9601 tgctattaat cgcaactatt tggatgaagt gcgtcttaaa gaacgcatta tccgcgagac
     9661 ccagccggac aaggtcaatc agattgaacg gataatgctg cgtaaaatag agaacgaaga
     9721 atcaggcaaa tggttccgca gataacctat gcgctgcggc cggattccgg cggggcggtt
     9781 gtccgcaccg tcggaatctg tgttgtcttt taggaaatta ggcagggagt ctattatgga
     9841 aacaggaaag acagtaggaa agttgatgct gtgggcggtt ggaatgctgg tgtgtgctgc
     9901 cggatgcgcc ccgagccatc agcagaacaa acaggcgatg acgcagcgtt gggagcagac
     9961 ctcggctcag gccaatattc cgcaggcaga agaacttata agccgggggc agcttgataa
    10021 agctcgtaaa atcttaaatg cctgtcttga cgtttcttca ccgcagccag cggtctattt
    10081 tctgatgggg cagatagata tgatggaagg ccgcaccgaa caagcccgtc aaaatctgca
    10141 acaggctgtt cagctggatc cggagcttga taaaggatgg tatttccttg gaacgctggc
    10201 ggttttggat aaggattatg tcaaggcaat ggaatacacg caaaaagcgc tggatttgca
    10261 gccggccaac acggactaca tccttaccct cgctgatatc tgcatcgaag gcggccgtat
    10321 cgagcaggcc gaaaagattc tcgatcaggg acttaaactg cagtccggca atctggatct
    10381 gatgctggcc aaagcacgtc tgctccagca gtccggcctg cggacacagg cgcttttgct
    10441 gtatgagcag gccctgctga tgcatggtgc tgttgcagcg attctggaac cctgcgctta
    10501 tgcttatttg gccgaaggac aatggcagca ggcggcggac aaattcgaac agcttctgcc
    10561 gctgtataaa caggatttgg accgatacaa tacgacgatg cgttcgctgg cgctagcgct
    10621 gttcaatgcc ggccgctatg gtcaagctct tgtttattat gataagctgt ctgtggtgta
    10681 tcgtcaagat gccgatatct ggctgatgat ggcgcattcg gcaatgggtg tggatgatgc
    10741 gaaacgtgcc ctgcaatgtg cccagcaggc ccttaaatac catccttctt gggcgagtgc
    10801 ttatgccgta agcggttgcg cccggtactt ccttggcgag tatgaacaag ctgtccaaga
    10861 ctatgccaag atcatcaatc atgaagaatt gggtgcattt gcatggttta tgaccggccg
    10921 ttgctatcag cagctggggc agccaattca ggccaacgcc gcttatgagc gtgcggaaac
    10981 aataaatccg aataatgaat tgattaaatc atttttggaa agaacactga agtccttgta
    11041 agggtgcatc gtgtctatga aaaaggacca attcatctgg atagctgctt ctgctgcagg
    11101 gctggcagcc ggcggcgccg caatccttgc cgctgtgctg tcgggcagcg tatcagtgat
    11161 tctgctggca atagtctggc tgtccatcgc tttgaccggc ggaatgctcc tgcatcatat
    11221 ccatcagctc aaagcgttgg agcgggtgtt tcgggtgggt tcgcagatga agattccgtt
    11281 tgagattgtt cccggatcgg tgctgggtga tatagccaat gcagccaatt gcctgctgga
    11341 tgagacgcat cagaagattc ggcaaatgca ggatgaaaac cgcgatctta tgctgcaaat
    11401 gcagctgctg cggcggcgca aaagcggcat cgaagcggta ctgaacagca ttcaagatgg
    11461 tgttcttgta accgatgaca aagatcgtgt tctgctggcc aatccggccg ctcaaaacct
    11521 tttgggtttt gaatttaata aagacaatgc gtgtctgatt caggatgcgg tgaattggcc
    11581 ggaattggtt caattgattg tcaaaagccg ccaaagcaaa atcccgcatg tgcggcatga
    11641 attgagccgt cagaaggatg gccggccgat gacctttgat tcggtactgt cctgtgtgga
    11701 agatgatgta ggtaatgtcc ttcagattgt aacagtgctc catgatatca cgcgggaaaa
    11761 agaaatctcc caaatgaaaa acgactttgt cagtcatgtt tcgcatgagc ttaaaacgcc
    11821 tctggcctca atcaatgcct atgctgaaat gctggtagat ggagaagcac aggatgccga
    11881 aacgatgcgt caattctgtt cggtgattca ggggcaggca cagcgtctca gtcggctgat
    11941 tgaagatata ttgaacattt cacgcatcga gtccggtctg gttaaggtca gccgccagaa
    12001 ccatagtatg gcgctggtga tacaggatgc agtcgatatg attgtcagct atgcgcagga
    12061 aaagaatata tccgtagagg caccggctcc cattctgtat gatcaggttt acatcgatcg
    12121 cgatatgata tcgcaggtta ttatcaatct gctcagcaat gctgtcaaat acacaccctc
    12181 tggaggtcag attgccattc gttccgaaat aaatgaactt gatggaacga ttactgttac
    12241 cgttacagat acaggggtcg gcattcccga aaaagacctt ccctatgtat ttgacaaatt
    12301 ttatcgggta gaagcaaata acaaatatgc taagggaacc ggattgggat tgaatctggt
    12361 caagcagata gtcgaatccg tgcacggcgg atctgttttt gcatccagta cacccggaaa
    12421 aggaagcacc tttggattta ttctgcctct ggctaaagcg gcggctgccc agatggtctc
    12481 ataagcaaaa ccagttgaaa taataagccg aatggagcga aatttatgga aaagaaaaaa
    12541 gcgctgattg tagatgatga gtttcatatc gttcaagtgg ttgctattaa gctccgaaat
    12601 aacggtttcg atgtcgcaac tgctgaaaac ggcagtcagg cctatgattt ggcctgccaa
    12661 actaaacctg atattattgt aacggattat cagatgccga tcatgaacgg actggagttg
    12721 atcgagaagc ttcgtcaaaa tccgcaaact gctgctattc ccgttattat gcttacagca
    12781 aggggatttt caatcgaaga tcggcttcag gaacagcttc gggtctcagc atgcctgagc
    12841 aagccgttca gtccacggga agttctggga ataattgagg aagtgctcaa aggacagaat
    12901 gtggcggttt gaaaaatctg ccatttcaac agaggaaaaa attctgctat gacacacaac
    12961 gcgagttcac aagccctgac agatgaaaaa atctatccgt ttcaatctct ggccgataag
    13021 ctcaatcggc tggatttgaa tctgctgctg tatgatgccg gcggccggtg tgtgctgcaa
    13081 agcagcgctg ggcgttttga aagtgatacg gaggccattg ccggccagat agaatcgatg
    13141 cagacggcac agaacggcct cctgctttcc ggctggcctt ccgacaattt gtttgtctgt
    13201 ccggtttcga ttgaaccggg gctgacggtc tttctggctg tggatacggg acctgccggc
    13261 tgccgtaatg ctgtgtgcga aaaacgctat cttgcctgta ttgtggagga tttctgcaaa
    13321 cagtttcagg atgcagaacg aattccgatg cagcttgaaa aaatcggtac cgagctgtca
    13381 cgcgcctatg aagaaattat gctgctgtat cggcttggcg ccaacatgaa agtgactcag
    13441 acgaatgcat cctacctgca gatggcctgc gaccagctga cgcagctggt gcatgttgag
    13501 ggcatcgttt tttttgtcga gaagaaaata gacggccgcc ggcagctggc acttatcgcc
    13561 ggttccggag ttatttcgat agaaccttcg caggccgata ttctgcagac gcggctgatc
    13621 gctgaactcc agcggggcag agaggccttg gttgacagcg ctgaagacag cccatttaga
    13681 tacgattggc ccccggccgt acgaaatatt atagccgtac cgctgggcga tgctgataga
    13741 atgctcggtt ttatggcggc caccaatata ttggataaac gtgattttga taccacggat
    13801 ataaagctat tcggctcggt ggccaatcag tgcagggtct ttattgaaaa taacagctta
    13861 tttgaagaac ttaaagagct gtttatcggc tgcctgaaag ctctgaccgg cagcatcgat
    13921 gccaaagacc agtacacccg cggacattcc gagcgggtgg cctgtatttc acgatggatc
    13981 gccgaacggc tggcgcagca tcggcctttg tccgacaagc agattcatca tatttatctg
    14041 gccggtctgc tgcatgacat cggcaaaatc ggcattggcg aatcagtcct gcggaagcgg
    14101 gggcggctgg atgacaatga atttgccgtt atccaatcgc atccccgtat tggagcatcc
    14161 attctttcgg gaatccgcca aatggacgaa attgtccccg gcgttttagg ccatcatgag
    14221 cgaatggatg gcaaagggta tccgaacggc ctgaaaggcg atcaaatccc cctgattgct
    14281 aaaattatca gtctggccga tgcgttcgat gcaatgacct cccgacgcgt ttatcgggac
    14341 gcaatgacca tccaaaaagc cctgtcggag atagaaaaag ggatcggcac gcagtttgat
    14401 gaagaagttg cccgagcctt cctcgaaagc gatgtagata agctgtggac tattattcag
    14461 gatggtttta ttgagagctg ggactacagc aattttgaag aatacggcat tgaagcggtt
    14521 ggagcactga tacgatgaaa atagaatcgc agaagtacaa cgatgttgtt gtgctgcagc
    14581 ttcagggaga actgacgggg gatgccctca agccctttga ggacgaagtc agcaatgcct
    14641 tggctgcccg catttccggt attgtgctgg atatgagcaa agtggtgttt attgatagct
    14701 gtgctctgga atttttaacg gatttgaacg ataaatgccg cgagcggacg cgccagatga
    14761 agattgccgg tcttgatgag acctgtgcca agatacttga aattacacgg cttttaaatc
    14821 agtttgatac atatggggaa ctgaccgagg ctgttaaaag ctttgtctaa ggacgcaagc
    14881 attaacgaat gaatacgact gtgcaaactc gattgccgct tggccaggtc ctgctcaaca
    14941 ggggagtcgt aacccaagag caggtcgacc aggccctgtc ccgccagcag gtaatcggcc
    15001 acagcaagct gctgggtgaa ctgctggtcg aactgggatt ctgcaccgat aaccagatat
    15061 gctcagcgct ggcagaggcc tatggtgtcc cctatgctca gctgacgcca aaactgtgcg
    15121 atccggcaat tgtagaactt ctgccgcgtg attttcttga agaacacgtc gtactgcctc
    15181 tgttcaaggt ccatgacatg ctgacggtag cactcagcga accgtccaat gtatttgtta
    15241 tcgaagagat ccagcggctc agcggctgcc gggttcagat tgtttgtgcc actgcacgcg
    15301 atatccgggc taccctgcag aattacctgc ctgctgccaa tgtatttgtc atcgatgata
    15361 tcattgatga tgtggctctg gaagatttcg cgatgattga aaaccttccg caggatattg
    15421 ccgatctgga agaagtagcg ggtcagtcgc cggtcgttaa actggttaat tatctgatat
    15481 ataccgctgt ccatgaaaat gccagtgata tccatattga acccgatgag aaaaagcttc
    15541 gggtgcgcta ccgggtagat ggtcagctct atgaaaaact gcgtccgcct catcagatgc
    15601 atgcggccat cgtgtcccgc attaaaatta tggcagaatt ggatatcgcc cagcgtcgtc
    15661 tgccgcagga cggttctatc catgttctgg ttcagtccaa gccggttgat ttgcgcgtat
    15721 ctgtaatgcc cggtatttat ggcgaaaagg ttgttatccg cgtgattgac ttccgcaaga
    15781 tgctgaccaa cctcgaatct ctcggattcg gatacgacaa tcttaagctc tttaagaagg
    15841 ttatcagccg ccccaacggc atcgttctgg taacaggtcc taccggctcc ggaaaaaata
    15901 cgaccttgta tgcggctctt tcagagctga acagcgaaca ggtcaatatc tgcacagtcg
    15961 aagatccggt ggaatgcaat atacacggca tcaatcaatt tgaagtacat gagatggccg
    16021 ggttcacttt ttccagtgcg ctgcggagtt tgctgcggca ggacccggat gtcattatgg
    16081 ttggtgaaat ccgcgatcag gatacagcca atatcgccgt tcaggcagcc ttaacgggcc
    16141 atctggtgct gtctaccctg cataccaacg atgcccccgg tgctatcacc cgcctgattg
    16201 atttgggtgt ggcgccgtat ctggtcagcg catcgctggt agcggtattg gcacagcggc
    16261 tcgtgcggaa aatctgcccc aactgcaaag aagaatacga gccggcccac agcatccgcc
    16321 ggacagtcaa agaatggaca ggccaagata ttgtttttta tcgcgggctg ggctgcaaga
    16381 aatgccgcaa taccggcttt atcggcagga ttgcaatcca tgagctgttt gtgccggatg
    16441 accggatact cgatatgatt actcaaaatg ttccgctgaa agccctgcgt gcggcggcta
    16501 ttgagaacgg gatggtgccg ctgcatatag acggaatcga aaaagtcaaa gctggcataa
    16561 caacaattga tgaaatcctg cgtgtagcca atgtgatgga ttaaaaaacg gttcttatga
    16621 cagtacaggc agtcgaaaaa gcacgaatga aaagcaaaca acatcctgcg gcagagacat
    16681 ccgcagcggg cgggacattt ttcaccggca gactgtctaa tctgcctgca ccggcctcgc
    16741 atgtccgagc caaaccgtcc gaactgatga tgtttaccac gcagctgtcg gtgatgctcg
    16801 acagcggggt tgtgcttagt gatgccattg aggcgattgc tgagcagatg aatccgggaa
    16861 cttttcagca agttgttctc gaaattgctc agcggcttcg caacggcgaa agtttttcat
    16921 ctgctttgca gaattggccc cggatattcg atacgatgta tatcagcatg gtcagaacct
    16981 ctgaggcctc cggcaaaatg gctgagatgc tcgatattct gtcgggctac ctcaatgcag
    17041 acgcagagac tcgaaaacaa gtcaaaagcg cgatgattta tccgatggtg atgctgctga
    17101 tggctgtcgc agcaaccggc tcactgatgt ttttcgtgct gccgcggttt acccgcattt
    17161 atcaggcacg gggggcggca cttcccaagc tgacgcaagt gctggtcaat tgcagcggcc
    17221 tgctggcaaa ttccaccttt ctggcagcgg ctgtaaccgc gggctttctg ggcttttggg
    17281 cgtggtctgt ctggcggcag acggaatccg gaagaaaaat tatcgattgg attaaggttc
    17341 acacgcctat ttttggcaca atgattaccg acagcattat gacccgtaat atgcggattc
    17401 ttgctacaat ggttaatacc ggggtcagtt tgctggatgc gctggaagtc atgaagacat
    17461 cctccgataa ttattatttt cagattctct ggacccacac agatgctaaa atccgcgatg
    17521 gctatcagtt ttcagatgct atccagatgt caccctgcag cggtctgatt gccccggcca
    17581 tcacccaaat gcttcgggca ggtgaaaagt ccggtcagat tggtcaagtc tgcgataaga
    17641 tttctgtctt tttggctaaa aaactccaga actccattcg aacagccaca gcgctgattg
    17701 agccgctgat gattactctt atgggactta ttattggtac gattgctatt gccctgctct
    17761 tgcctgtctt ccgcatctcc agtgtgatgt cgcactgaca aaatctgccg ctcttgcatt
    17821 tacataacat cgcaaaatgc ggcagtcgaa agatcctttg ctccaagtct gaataggttt
    17881 tctgtttgaa gagcaatgat caaaaaggat gggtttgcag tgttgcagaa atgcagatat
    17941 cgctgtcaaa ctgttcgatt tgaacggctt gcagtgtctg gtatcctgct gtctgagaca
    18001 gaacaagtcc caattctgct gttccgtcgg tgccaagaat gaggggagct atgaaaatgc
    18061 agacttcatc ggccagctgg tgagccaaaa attggcctat cagatgcggt cctgcttcaa
    18121 caagcagttg ctggatgccc cgctgcccaa gctggctaag ggtattcttt aaatcgcagc
    18181 gtccatgttg ctgtggaacc gtaagcacct caatcccggc ttttttaagt gatgcgatgc
    18241 ggttttgcgc agactgggca gtttcctgtg tcgtaacaac aagtgtcggc gcatcggttt
    18301 tcgttaaaag gtgggattct gccggtattc gcaatctgct gtctaacacg atccgcagcg
    18361 gcggtcgttc gactctgacg tcctgaaggc gaactgttaa atcgggatta tcggcgatta
    18421 ctgtgtcaac gcctgtcagg atgccctgta cttttttgcg aaggtggtgc actttctgcc
    18481 ggctcttttc ccctgaaatc catcgttttc catgcatgct tttccatgcg agtttgccgt
    18541 cgatactttg tgcccatttt aatataatcc atggcaaacc agtgcgggca tgcttataaa
    18601 agggggcatt cagcatttca gcttcttggc ggcacaagcc ggtgtggacc tcaatgccgg
    18661 cttcgctgag ctgtcgaatg ccgccgcctg ccagcggggt agggtcttcc gatgcaatca
    18721 ccacgcgctg gatgcccgct tcgattatcg ccttacagca gggcggcgtt ttgccgacat
    18781 gggtacaggg ctcaagtgta acatacatca ccgctccctc gggagtatgt ccctgctttc
    18841 ggcagtcagc caacgcatta atttctgcat gcggtccgcc aaaacgctca tgccagcccc
    18901 ggccgattac agtgcccttt ctgacaagaa cacaccccac ggccggattg ggttcaacct
    18961 ctcccaatcc cttgcgggcc agctccagag cctgcttcat aaaatcaaaa tggtcctcgt
    19021 atctgtcttt ttccattcca tagaattata gaacatttgc tgctgatgaa actccttatt
    19081 ttaaggatgc cggtgcccat tcgacttgag cagatcccag aagagtacca gtcgcatagg
    19141 caaggtcaac cagcgaaatc tgatattctg tcagtgcatt gatttcggcg ctctgggcgt
    19201 tggcaaaatt ggcttgggct gccagtacat ccgttgaggt tcgaaggccg atttcaaatt
    19261 gacgaatttc agcctgataa agccgttctt caagcagagc actttgccgg ctggccagaa
    19321 tccgctgcca gttggcttcg cattggtcta aagcatttag aacttcgact tctatcattg
    19381 cctgccggtt ttctttggtc gccaaggcct gctgccgctg gaaaaaggct tgccgcagcc
    19441 ggctttcagc cgcttcattg cccagtggaa ccatcaattg gaaacccagc cgatggtcag
    19501 caaaattatt gcgccccagc atatcccagg cggcactgcg tgccggtccc agcccattga
    19561 tattgtacgt gtaatcaagt gtcaccagag gtaatgcctg attgcggaga tagtcaatgg
    19621 tgctgatgct tttggcaatt tgtaattcct gctcgagcat ttccatacgg ttttcaattg
    19681 cttgggatac cagcgtcggg gctgagaagg tataatgaac cggattcggt tctgtggcag
    19741 gcagaatcac cgtcggtccg gccggctgga gatctttctg ctgaagagcc agtttcagtt
    19801 ctcgctggcg gtcacgaagg ttgttttcgg caataataat agcttccaga ctctgggcca
    19861 aaccggcttc agcccgcaca atttcaatcg gaggatgctg gccggcctcc acaaagcggc
    19921 gggcacgttc cagctgtgct tgagccaggt catactgctg cttgcgaacc tccagctgct
    19981 tgaatgccgc atacagacgc cagtaaacac ggtcaacggc ggcaataacc cgaatgactt
    20041 ccagtttggt gcgggcatcg ataatgcttt tgtcgtatgc agcaatgcgg atagaataag
    20101 tattggtgcg aatgccggca ttgcgaagca gcggctggct gatagagaga gaaaagtcct
    20161 cgctgtacga aggattatag gttgctgact ggaaattgct ctttgtgcgg ctgtcggcca
    20221 tgtcgaatgt aatggtgccg ccggtttgaa gcggaatccg aacacccaga tccacgtagc
    20281 ctgaatcaat ttgagaacct tggatagcta aggcagcagc cggcggctgg tctgttttgc
    20341 tgtaagacag attggaaaag aaagcggcct caaacttcgc ccgttccgca ttgacgccct
    20401 cttcggcgat gcggggggcc agcaattgga ctttaatatc aagattgttt tcaagggcct
    20461 gagagcgaca ctgctccaaa gcaagcgtca aagatgccgg cggctctttg ggtgcttcca
    20521 gtacggaggg aacagccggc tgctcggcag aacggtagga ttccagatcc agaggttcga
    20581 tgatttgcac tttctctggg gcagcctgcc gcggagaaaa gggggcagaa tactcgccgc
    20641 agccggttcc aagcaaaagc acccaacaga ggatctgcat atttacccat tttttttcca
    20701 ttgttttttc ctgtttatta ttcatgccgc agtgcatcga tgggattcag gcgagcggct
    20761 ttgatggcag gaaacatccc gaaaataatt cctaccgccc ctgcaaatcc aaacgacaga
    20821 acaatcgccc acgccggtat ttgcgtctgc tccatcaggg gagcggattt ggaaatcagc
    20881 ttggcaaaac cgtgtcccaa gaaaaccccg atagccccgc caatcatgca cagcacaacc
    20941 gactcgacaa gaaactgtgt caaaatagca gatggcttag ccccgaccgc tttacgcagg
    21001 ccgatttccc gcgtccgctc agacaccgac accagcataa tattcataat gcccacgcct
    21061 cccaccagca gcgagatact cacgatgcca aaagccacca gtgtaatcat acccatcact
    21121 tttttaaatt gctggacttc actttccatc agaaaaacgc cgaaagtatc cggctcaccc
    21181 ggctttatgt ttcggctgcg gcgcagaaaa aattgaattt ctgcctttgc ttcttctgcc
    21241 acctcggcat tcttacagct cgctataacg cggaaccatt gaaaatctgt tttatttatt
    21301 gttcggaaag gaacaaaaac ctcgtattga ttttcacgca tattaccgaa catcatagcc
    21361 ggcttgtctt cgagcagtcc aatgatgaga tagggagtat atcccagcag aatgctttgg
    21421 cctatgcagt tgttatccag attcaatttg tcccgcagat ctgcggttat aatacagact
    21481 tttcgtccct gcgtctcgtc caaaaacgaa aacggtctcc ccatcacgat attgcgattt
    21541 tcgattttga accagcttgc ctcaacaccg gtcaaacgga cagattcaac tgttcgatcg
    21601 gcgtatcgag cattgatatt ctgggtatcg gaaacgcagg ataaatattc gacggaaggg
    21661 cagtgttcca gcagcccctc gaactcctgc gttcggaaac ggatatccca ccaaggagtg
    21721 ttctttttgg ggcctgttga gggccgctgg ggccaaagaa agattttttt tgtgccgaat
    21781 atctcaaaat tctccataat tttcttgttc atcccatcca ttgcagcaat gacggctgaa
    21841 accgatgcga cgccgatgat aatgcccaat gtggtcagga tgctgcgggt tttgttaacc
    21901 caaatctgat tgatagccag cagcgtgctt tgtatcagca gccgcaatat gaccagaggt
    21961 gttcgaagca agaacctcat gcatcagctc cttttgatgc ctgctgatca ctgaaaatct
    22021 gcccgtcttt catcgcgaca atccgccggg cgtgggcggc aatatctgct tcgtgggtga
    22081 ccataataat cgtctgtcct tcctgatgga gcttctcaaa aagctgcata atgccttcac
    22141 tggttttgct gtcgaggttg ccggtcggct cgtcggccag cagcagactg ggactgccca
    22201 ccagtgcgcg tgcaacagca acccgctgtt tttcgccccc gctgagctgg ttgggcttat
    22261 gatgagcgcg ttcagccaag ccaacacgtt ccagtgcctg catcgccaat ttgcggcgcc
    22321 gcaggaagga aacatcggaa taaatcagcg gcagttccac atttttcaga gcgctcatcc
    22381 ggggcagcag ctcgaatgat tggaatacaa aaccaatttg ctgattgcga atgtgggcca
    22441 gctgcgaagc ggtcattttt gtcgtgtcaa tgccgttgag ttcataggta cccgaatctg
    22501 cacgatccag acagcccagc aggttcatca gggtgctttt acccgaaccg ctgtgtccca
    22561 taatggcaac gaattcattg gttttaatgt ccagattgat gcccctgagg gcatagaccc
    22621 ggtcggcgcc cactttgtaa gtgcgggtca gatttctgac gcgaataaga atggaagaag
    22681 ccaatgtatt gctcatagag attttttatt ttttgccgcc gttatgtctg aatctgcggc
    22741 ttcttttttc ttttcttctg tgacagccgt atcgtgtttg agggtttcga gaatcttata
    22801 aggtcctatg ataatagcat ctcccggctg aaggccttcc cggatcagag tatgcgtcag
    22861 atcgctgggg ccaatgtgta cgggtgtggc aaccgccttc ttatttatat agcgaaagac
    22921 cacaggaatt tccgtttttt ttgcatcaac tacaggattg ttatctatta tatccagcgg
    22981 cagttcatca acacgccgtg ccaaaacggc ctggctgggt actttgataa cattttcata
    23041 acgatttgtt tcaatatcca catctgctgt aaggccggaa tagagtttac cgacttcacc
    23101 ttctatcagt atttcagttt tatagtattt agcaccaatg ctggacatgc gatgcgtcaa
    23161 ggcaatgttg cgaacaactc cttgaaattc aacatcccaa aacgcgggaa catgaatttt
    23221 ggctcgctgg ccgattttaa cttggccgac gttggtttca tccagttccg ccagcagaat
    23281 cattgtcgac agatctgcaa cttgcataat cacagttccc ggattgttca tcgtgccggt
    23341 cataaccact tcgcctactc cagcattaat ttgggtgatt acaccatcaa tcggagccgt
    23401 aatagtcgta taactgaggg cctcccgaac ttcctcaaca cgggcttcag cggcgctgag
    23461 attgtgttcc attacaacaa gattcagttt cgctgcttca agcgaatgca cggccgcatc
    23521 aaattgcgcc tgcatctggc ggaacgagct ttcggtttgt tcgaagctgg attggctgat
    23581 gtctttggtt tccagaagtt ttttctgccg ctcatactcc agtttggcac tatccagaga
    23641 tgcttttaac ccttctaaat tggcttgctg cgaaagaaga cgtgcttttt ctacctcaat
    23701 aagagctcgt tgagcatctc gtccagcttc agcgctgcga agctgacttt ccaaatcccg
    23761 tgagtcaagc cgaattaaaa catccgccgg aacaggcgga ttcgcagcag ggtctcctgc
    23821 agttacggtg tcaccttctt caaaaggcaa ttcttcgatg cgggcgctta ctttggcgct
    23881 gatatcgact ttggtttttg gtcgaatttc tcccggggca ttgacaattt cgacaaattc
    23941 gctcggctga acagtttcta agcgaacttg aatagattgt atatctgttt tacgcttctt
    24001 tattattccg gctgataata taagaagcaa aacaaccaca atacacaaaa caataaatac
    24061 cttcttgctc accgtaaatc ccttaatttg gatattcata ctttagaaaa ataacgcact
    24121 aatttaagga atgttaccat aaagacgaaa agttgtgaaa aatataaatt ttttgttgaa
    24181 aatagcgaac aaaatatgca ttatttgtaa tatataaata tatagatatt ataatgtaag
    24241 gcgacaaata tgacatacga agcggctcag tatatttccg agatcttaaa ggcagttggc
    24301 catccagtcc gcctgcagat aattgaacag ttaagagacg gaccgatgtg tgttggactt
    24361 ctgtcgcaaa aactggatat gccgcagtct gttatcagcc agcaattggg gttaatgaaa
    24421 gacaagcaag ttcttcaatg ccgtcgggag ggcacaaagg tcttctacag catcaaaaat
    24481 cttaatgtta tcaaactgct ggattgtgtt tacagccaat gcccagcgcc agagcaatcg
    24541 gcatagaagg aagaataaaa tgtattgatt tattgggtaa gagaatatct aatggcaagt
    24601 tattattttg cggagtctcc aaaatgaagg ttttcagcga atctgttgtc caatggtcgg
    24661 tggcccatta caaacttgtt acggctgcaa tgattgtatt tacattgtgc tgcggggcat
    24721 ttatacctct gattcaagta gatactgacc cggaaaacat gctttcgcct gatgaagcgg
    24781 ttcgggtttt ccacaatcaa accaaaaagc agtttgacct cagtgatatc gttgttcttg
    24841 ggattattaa tgaacgcaat gaatatggag tatttaatcc cgcaacgctc cagcgcattt
    24901 atgaattgac ggaatttgcc aaaactctca accatctgga caaaaaggat ccgcagcgtc
    24961 tagccggcgt gattgaagtg gatatgattg ccccttcgct gattgaccat atcgggcagg
    25021 gcggcccggg agtgattaca tttgaatggc tgatgccgca gcccccgcag acgcaggccg
    25081 aggcgctgga aatccgcgat aaagccctgt ccaatcctct gctgaatgga acgatggttt
    25141 ccgaagatgg aaaggccctg tgtctctata tccccctgac agataagcat ttcagctatc
    25201 gggtgtattc ggagctgaac aaaaaaatcg ctcaaatggg cggtgaagag aacttttata
    25261 ttaccggtct gcccgtagca gaagatacct tcggcgtaga aatgtttatt cagatggccg
    25321 tttcggctcc gctggcgatg ctgactattt ttatcctgat gctcattttc ttccgcaagc
    25381 tcatcctggt gatagctccg atgattatcg cgatggtgtc agtcatctca accatggggc
    25441 tgctgattgg gttccgttat ccggtgcata ttatgagctc gatgataccg attttcctaa
    25501 tgcccattgc tgtggtggat tcggttcata tattgtcaga atttttcgaa gtttatacca
    25561 agcaaaaagg acgcagacag gccgtcttgg atgttatgca ggttttgttt gctcctatgc
    25621 tctacacttc cttaacctca gcagccggct ttttatcgct ggcgctgaca ccaataccgc
    25681 cggtgcaggt ctttggtgtg tttgtggctg ttggaataat gattgcttgg attttcaccg
    25741 tgacatttat accggcctat gtgatgctga ttccggaaaa gtttctggca actttcggac
    25801 gtgctgtgtc tgatgaagat aaacccggca tcatgacgcg tctgcttcat agcgcaggcg
    25861 gcttaacctg ccgttatgca aagccgattt tagccgcact ggcagtattg acgggtttgg
    25921 ctggctgggg cattgctaaa attcaaatca atgataatcc ggtcaaatgg ttttctaaac
    25981 gccaccccat ccgcattgcc gacattgcat tgaacaggca ttttggcggg acttatatgg
    26041 cctatctgat tctggatggc cagccccccc aaaaagcaac cacagaagac ttggaatata
    26101 tccgtaaggg tttggcagct tttgcagccc agattcagca ggattatgcg aatgccccta
    26161 ttctggcaga aaaaatccaa atccagctgg attccctctt tgcagccagc gattcctata
    26221 agggattttt ggatgccgtt gtccgatgga ttgatacgca ggctaagaat gctcccgatg
    26281 aggaatatta tacgtgggac gaactgcgtg tatattttgg catagagggt gaacggctga
    26341 aggtcttcaa acgccccgat gtcctgaatt atatggcggc tctccaatcg catctggaac
    26401 agactggttt ggtgggcaaa agcaattccg tcgccgatat cgtacgcaaa atcaatcagg
    26461 aactggtgga cggcaggcct gagaattacc gtattcccga acagcttcag ggtgtggccg
    26521 aatgttatgt gcagtttcag cagagccatc gcccgaagga tttatggcat tttctcaccc
    26581 cggactacct ccacgccaat atctggatgc agcttaccag cggcgacaac aaagatatgg
    26641 aacaggttgt ccgtgcggtc gaggactact ttgctgcgca caagccgccg gtggagctga
    26701 cctatcactg ggccggcctg acatatatca atacagtatg gcaggagaaa atggtctttg
    26761 gcatgctgca gtcatttttg ggctcttttc tggtggtatt tatcatgatg gctgtgctgt
    26821 tccgctcccc cctgtgggga cttttatgca tggtgccgct gacgattacc atcgcggcaa
    26881 tttatggaac gatcggcttt atcggcaaag attatgatat gccggtagcg gtcttaagcg
    26941 ccttgacact tggtatggca gtcgattttg caattcattt tctccagcgg agtcgggaaa
    27001 cccatcaagc ggccggatcg tgggcaaaag cagcccctca aatgtttggc gaaccggccc
    27061 gtgctatcag ccgcaatatc cttgtgattg ccatcggttt tctgccgctg ctggctgctc
    27121 cgctggtgcc gtataagacc gtcggcatct ttctgtgtgc gattatggct gtttccggtt
    27181 ttattacgct ggcggccttg ccggcggtgc ttactgtggc agagaaatac tttttcaaag
    27241 ccggcagaaa aatagaatct gttggctgca actgcagttt ttgcatcatc atcgccgtct
    27301 cggtagcagc gctggttgca cttaatttgc atcagtactg gcaggtaggt tttacaaaac
    27361 tcacagggat gagtattgct ctcattccag tcttagtttt ggtttgcgga ctgatgtctc
    27421 gccggaaggc gtgccagaca aatagagaca atcaaatttc acgggaagat ggaggtgtca
    27481 aatgacagtc gaaaaagcac tgagggcaat agccggcgga tttgtattga ctagcgttct
    27541 tttggcttcc ttccacagca ttcattggct gtggtttacc ggatttgtgg gggccaatct
    27601 gctccagtca gcatttacca actggtgccc gatgatagtc cttctgaaaa aacttggttt
    27661 acccagcagt taacagcaga aagcgaggta tttgaaaatg aaaaaactaa tttttctatt
    27721 ggttatgaca gcagttcttg gattaagaac atttgcccaa gagtcagccg ctccaacagt
    27781 tgaagaaatc gttaaaaaag ccaatcatac ggcctattat cagggatttg acggaaaaag
    27841 ccgcgttgta atgaacatta ttgacaagca ggaacgaacg cgcagccgcg agtttgttat
    27901 tcttcgcaaa aataccggac aaacggacgg ggaccagaag tattttgtct attttcaatc
    27961 gcctgctgat gtccgcaaaa tggtgtttat ggtgcacaag catgcgtcgg tagatcagga
    28021 ggatgaccgc tggctctatc tgccgaacct cgatttggtt aaacggattg ctgcaggcga
    28081 caagcggacc agctttgtcg gttctgattt cctttacgaa gatgtctcag gacgcaattt
    28141 ggctgaggat actcacctgc ttgttcaaac caccgaccag tactatgttg taaaaaatgt
    28201 ccctatcaaa cctgaaacag tagagttttc atcctataca gtcttcatcg ataaaaaaac
    28261 ctatatgccg atgaaaatgg agttttacga taaagacaac gttctttacc gcacaatcga
    28321 atcaataaag gtcgaacaga ttcaggccaa agaagcagat caagaagtaa cctatccgac
    28381 ggtaacccag tcagtagtca aagaccttaa aacgggcagc cggacagaaa tgacattctc
    28441 ggatattcag tataatatcg gattgtcgga tgatatcttt actgaacgtt atctgcgcag
    28501 acctccaaga gaggcaatgc gctgatagga gccgattatg aaagtaaaat ggatttggat
    28561 gaccgtaatt ttttttagta ttggagcagg ggctgttgct gaagaaaatg acggcgcatc
    28621 gccatggcag cagttgttca gctcggtgga tgttcacgga tttaccgaac tgcgaggcgg
    28681 ctaccgcatt cgcaaggatc cttaccaaaa ggatatgtcc gtaatggaag cccgtaccca
    28741 actggatttg tccagttata acgattgggg ggatttcaaa tacaagggag atatctgggg
    28801 agatcttgta gccgaacagg gcgactatga aacacgcgaa ctttggtttt ttacccggcc
    28861 agccgactgg atggatttca agcttgggcg gcaggttctg acttggggga caggggattt
    28921 attattcctg aatgacctgt ttcctaagga ttggcagtca ttttttatcg gacgtgacac
    28981 cgagtacctc aaagcgccgt ctgatgccgc caaattcagt ttttttacag atgcggccaa
    29041 tattgatgtc gtttatacgc cgaagtttga ttccgaccgg tatattactg gccaatatct
    29101 ttcttattgg aacaattctt taagccggcg tgcgggacgg gacgcctatg tttatactga
    29161 caagccggac cgttgctttc gggatgatga aattgcactg cgtatttata aaaatatcaa
    29221 caactatgaa tacgccctct acggctattg gggtttttgg aaaagccctg ccggaatgac
    29281 cccaacggga tcggcaacct ttcctgacct gaatgtctat ggcgccagtg tccgcggaca
    29341 ggtcgccaaa ggcatcggca atgccgaaat cgcctattat gagtccgctg atgaccaaaa
    29401 tggaaaaaat ccgcttatca gcaactccga attacggtat ctggttggct atacacagga
    29461 aatcgccaaa gatgtcacag gcggactcca gtattatctt gaacagatgc tcgattacaa
    29521 tgcctatctt cgccatcttt ccggcggtcc gaaacgagac cgggaccgcc atgttttaac
    29581 agctcggctg accaaacttc tgatgaatca gaatctgtcg ctttcgatgt ttacctatta
    29641 ctctcccagc gacagcgatg tctatctgcg gccgaatatc cattacaaag ctactgatac
    29701 cttatcggtg gaaactggag ccaatatctt ttttggagac catccgcaca catttttttc
    29761 ccaatttgag aacaactcta atatttatgc ggctgtccga tatagttttt aatggcgtcg
    29821 caatgggata tttcaccatt ttttcaccag aaggtatata aagtgggaaa gtttattaac
    29881 agataacctt caaaatctga aatttttaca aaattttccc cgctttcttg ccggcacctg
    29941 ttagaaaatt catacaaact atatgtagtg gccattgtct atttttgggc actatatatg
    30001 gtaattttct tgttttagac gagattttgg atatttatct cggaagattt ttttaacata
    30061 tttttgaaaa aaaccgatgt ttacttgttg acattgtggg aataagtggt aataattccc
    30121 acaaacaata ttagctgtgg gacaagtacg atgctgctgt tgaccggcga atacgaacat
    30181 acaatcgatg agaagaatcg gctatttatc tcaaacaaac tccgaagtca aatcgatatc
    30241 cggaaatacg gctgcaattt ttatcttacc atgggttcca acggcatttt gtgtttatac
    30301 ccggatgaat atttcgagca gatcgccctt gccggtgcgc cacggacagg cgccccggac
    30361 gaagcagttg ctttcgagcg gctcagcttt gcattggcca gcaaggtcga gttggatcgg
    30421 cagggacgat tgttaatcaa tgaaaagtta agaaaacgag ccaatctggg tacggcattg
    30481 actctggtag gggtccgtga ccatattgag atatggaata cggaagactg ggagcattat
    30541 ctggaagata atctcagcca atttcataca caggtggctc aggcacgcca agcccagctg
    30601 caaaggcagg gccaggattt ggaacgttag ggagaactcc ttatgaagac acaaacaaac
    30661 aatccagtag aagtgcttca acagggactg accgggcggc gtccggtgga tgagcaggtg
    30721 ctgtcttgtg cggcactgct ggccgaacgc ctccagcagt taaaaagtgc cgacccgatt
    30781 tttgactcga tcgcgttttc gccggatgtg gaggcaatga tggcttccca tctgacggcg
    30841 gttgtaaatt aatggtttct ttgacaatcc ggagtgtccg atgtcggttc atcaggacaa
    30901 agggatagat gagccgctgc cggcggaaca tattccggtg ctgctgaaac cgcttctgga
    30961 tatcgttcag tttcccacgg atgggattat ggtcgatgcc acggttggac atggaggtca
    31021 cagccggcag ttcggccgcc gcttaggccc ggagggattg ctggtcgggc ttgatgttga
    31081 tgcagagtgt atccgtcgag cccgttcatc cctgtccccg ctggcctgtc gggtgcttct
    31141 gctgcgggag aactttgcag atatcagcac tgttctccgg cagcagggta ttgacaaggc
    31201 ggattttatt ttggcggatc tcggtttttg ttcggcccag atggacgatg tccagcgggg
    31261 tttaagcttt caaaagccga tgccgctgga tatgcggctg gatgaccggc tgacgacaac
    31321 ggcagcggat ttagtaaatc agctgagcca gacggagctg gcagacgtga tttataggta
    31381 tggacaggat cgagcatcac ggaggatagc tcgattcatc gtccagcagc gttcggttcg
    31441 aaaattaaca acaaccacgg aattggcaga gctggtatgc cgggcactga agcaaaggcc
    31501 ccatgcgggg cggattcatc cggctacacg gacatttcag gctctgcgga tcgctgtcaa
    31561 cagagagtta gaaaatctgg atcggctgct ggagactgca cccgctttgc tcaagccggg
    31621 cggatggatt gcagttatca gttttcacag tctggaggac ggacgggtta aaaacaattt
    31681 tcgaaaaaat catgcggacg gacggtatga gatcataacc aaaaaaccaa tcgtagcgga
    31741 tgcacaggaa gttgctcaaa atccgagagc cagaagtgca aagttgcgga ttgccagatg
    31801 tttgtgagat gagaaaagga gttttcaaaa tgactgctga tgcgaaaata ggattgctgt
    31861 tggggctgtt ttttattgtt attattgcat ttctggtcaa tggactgccc aattttgtgc
    31921 gtcagaacga ctcaaagccc cttgatgcgg ctattaccgc ctctgggagt tcagaactga
    31981 ttattgataa cagcttgacc gaagcgattc atcgcctccg tcctcaaatt gagctgcgcc
    32041 agacagagcc ctccaatgaa gttttagtta ttgattcctc tgcccagccc ataccgcagc
    32101 aatctttaga aacggtatct cagccgcaaa tgaaagtcca aaccgaagtt gcggcagcag
    32161 ttacgccggt ccctgctccg aaacaggaat caggtatcca agcagtgcag ccggcttccc
    32221 gctccgtaca gccggtttcg caaaagttta aaacccatat tgtgcaatca ggcgagcatc
    32281 tgtcttctat tgcgcagaaa tattacggca aagaggacgg caatcgccga ctggttattc
    32341 agaaactata tgagacaaat gcaaagacct tgaaatcccc cgatcatatt caggcaggag
    32401 ataaactggt tattcctccg ctggacgaac tgctgggagc accgcaacct tcggtttctc
    32461 agccttctga gacgcttctg agtaagtttt cttcgctttt ggaacgagca ggcaaagagg
    32521 ataccaagaa ggtatctgaa tatgtggtgc ggcaaggtga cagtttgtgg tctatttccg
    32581 agaagacctt gggcgacggc aagcgctaca atgaaattat tcagctcaac aaagaccgca
    32641 tcaagaatgc ggacgatgtg gccgcaggca tgaccttaaa aatccccgct cgatgagccc
    32701 agccggatgt cgcgattgcg agtcattttt gttgtctttt ccataacggc atgtttggtt
    32761 tttaccattt ttttgcggac ttccgccagc cggatgttca atcaatatcg cagtgcggag
    32821 gtagagcaga aaaaccttcg ccagcagctc tggcagaagc agcttcgttt tgaatatctg
    32881 gtcaatccgg ctggattgcc tcccttgcct cctccagatg attcggctaa gaaaggcggt
    32941 atgcaatgac gtactctcga cgcgaacgct ggctgctcgg gctgttttta gtgctgattt
    33001 tgccggcctt tggaggactg atatggcgtc tgtttgattt gcagtactat caaaaagaga
    33061 cgtttgataa agtcagccgc aggcagcaga acgccgttgt tccagataag ccgcgccggg
    33121 ggcttattct tgattctcgc ggtcgagtac tggccgccag cgttaagaca tggaacgcat
    33181 ttgccgaacc gcgccgcctg cgtgaatatc cagaccagat acaagtggcc gcctcggctc
    33241 ttcgggatat cctgaatatg cccggctatg agatttgccg gattattgaa gaaagccgaa
    33301 atcccggcta tgtcaagctc aaagcccagc tcactcccgg ccagcgggat ttgctgagtc
    33361 actgtcgact tgccggcatc ggtgttgaag ccgactggat tcgccagtat ccggcggggt
    33421 cgttaaccag tcatctgctc ggatttgtag gtgctgaaaa tacaggctta tcaggtcttg
    33481 aattgcagta tgaaaatatc cttgccggtc agccgggaca gcaggttttt atcgttgatg
    33541 cccagcgtcg tcccatcggc actcaaatgc aggaagctat tgaagcccgg gatggcctga
    33601 atctggtatt gacaatcgat tcggtcattc agcatttcac ccgcgaagcc ctgctgaaaa
    33661 aaatgacaga ataccaagcc gaatcggctg tggcgatggt aatggatccg tggacgggag
    33721 ccgttctggc gatggtatct ctgcccgatt atgacccggc ggctttttca actactcccc
    33781 aagaccgaat gcgcaaccgc attttaacag attcctatga acccggaagt attttcaaac
    33841 ccattgttgc agctattgcc cttgatgcgg ggtctgtagg atttaatgaa aaattcgatt
    33901 gtgaaaacgg ctactggccg cgttatcgaa ttggggagtt tggcaatcat caatatggca
    33961 ttttctcagt ccgtgaaatc ctgattcatt caagcaatat cggtatggcc aaaatcggtc
    34021 tgaaaatggg tcagaaagag ctgtataagg gcttaaaatt atttggattt tctgagcaaa
    34081 caggtattga cctgccgggt gaagaaccgg gtctgcttcg tccattgtcg caatggagcg
    34141 gctggagcgt aacccgtatt cccttcggcc atgagatatc ggtaaccggt gtccagatat
    34201 gccgggctta cagcattttg gccaatggcg gctatatgat taaaccgcat atagtccgtg
    34261 cagttgttga taatcagggg acagttatcg aaaataagca gccgcctgtg agaacgggct
    34321 atattcttaa atccgaggtg gcacaatgga ttgtacgcaa tgccttggtc ggtgttgtca
    34381 acgaaggaac cggcgatcag gcaaaaatgg aacagataca agtctggggc aagaccggca
    34441 cggccaatat tgctttgtct gggggcggct atgatacccg caattatgta gcatcctttg
    34501 tcggcggggc gccggccgac aaaccggctg tcgtggttct ggtgtctata cgcaaaccca
    34561 atcgcgcttt gggcaaaggg tacagcgggg gacgtgtagc cgcaccggtt gtaaaggaaa
    34621 ttctcgaaaa caccctaact tatctgggca tcatcaattc tcagcagctt ccggtccggc
    34681 aggtggtgca gcagtaggtt tttcacctat atatcccgcc tcgttgcagt aatacagtat
    34741 ccgatggccc agcggagccg cttccccggc acctcgctgg ccgccttcta caagaacagc
    34801 caccacgatg gtcctgccgg ttttatcttc ggcaaagcat tcaaaccacg catgataagg
    34861 tctttcggtc gaaccggtct tgccgtagat ggttatgccg cgactgaaga gttcgctgtt
    34921 tctgaattcc tcgcgggctg ttccatgact ttcataaaca acggccttca ttccatcccg
    34981 cacaacagag agtgttgacg gcgaaacagg tattcttctt tggtaccgct cgttgtgcgg
    35041 gtcgcggtcg tcgaagacca gacggggcgc tttataaatc cccccgcggg caatggcaga
    35101 aagggcgtta gctgtctgca gcactgtaac ccgcaagctg ccttgcccca ttccccacca
    35161 ttttttttcc cagtccggaa taggtttaag ctctgaagcg ttttgaactt tcggctgata
    35221 gacatcatag atcaggcatc cgcgcgattg gttaagaagg atatcctcgg tattgtttcc
    35281 tgcggcctca gacggcagcg gcgtcagcag aacatcttgg cccaagccga actgaaaaag
    35341 ccaagattga agcgcctgcc ctttaagccg attggctaac tgagaaaaat aaacattgca
    35401 gcttccccgg atagcatttc gtgcgatatt gcctccctca ccggcaaagt ggtcatcgtg
    35461 accgatgccg taattgcgct gcagcagaca gcgcggccac ccctcaggcg gcagatgcca
    35521 tccggaacag gaaatcggct ccgtcgggga gattttatgc tcttccaaac cggctatcag
    35581 cacaaggggc ttggcggttg aaccgggggg ataattccgt tccaatgctt tattttccca
    35641 aagcttgttc ggatcccgcg gattaaagat gcgattgtaa ttgtcgcttt ggcgaatggt
    35701 attcaaatca aataccggaa tggatgcaat ggccagaata tcattattgg ctgcttccaa
    35761 aaccacagca gcgctgggtt tgccttcgtg aggcatcctc ttgtcggcca aaagtttttc
    35821 gattttctgc tgcaaatcca tatcaatcgt cagctgcacg tcccggccgt actgcggttc
    35881 aatccgttcc agaagattgc cgtcccggtc ataagtgacc tcgccgcgcc ggccgcgcag
    35941 aacggattca tagatttttt ctatcccgct tttgccgata acttccccct cctgatagct
    36001 catgtattta tcatgctcaa actgctcgat ttcgtgctcc tgccacgggg cgacccagcc
    36061 gataatctgg caggcggctg ccccataggg ataataacgt ttggcttctg ttttaatctc
    36121 cagttcttga atgcccagca gctccacttg ggcacgcagc aggtcattct gtgttttcag
    36181 ctcaattaga ggatacgact gatgcatttc ccataaatca atctcgacaa tatcttcagg
    36241 ccttatcagc tcccgctggg caagataatc ggcccgcgag cgtccttgat tgcgccgcag
    36301 ccagaaaata tagcgtgccc gttcccagat aaagtcgtta atctgctgaa tattggctgt
    36361 gatttcctcc cgagatgcat ccgccagctg caaagccagc tcaatcgttc tattcagggt
    36421 ttccagcggc tctttccatt tttcctcata aaactcgcgt tcgatctcct gccgggattt
    36481 gctgtttgta acggcccgaa ggatgcgtcc ttccctccag cgggcatcca tcaggcgggt
    36541 cagctcataa ttgatgtgaa gaaaaaaagc gggcttatcc aaagcgaccg gattgccgct
    36601 gcggtccaaa atccgtccgc gaatcgtagg acgctgctgc ggaacaagga tgcgcagttt
    36661 ttcgacttcc tcacgggctt gctggacatg acagatttgc agaatcataa gccggctgac
    36721 agcgattgtc agaccggcca gacagagcag aataaagatt ttaaggcgaa agtgatacat
    36781 cgttattcat tcagaccgga caggctttcc cttgtgcctg ctcctctgtt taggatggat
    36841 aagaaggacc aaagcagcgg gcttatgacc gccgaataaa gtcccgtcag cagcacagtc
    36901 gagtagggaa ttgtttgacg cactttaagc attcccagcc agagggtgaa agtttcggct
    36961 gtcagataaa cagcaaaaat gattccggct cgataaagtg cctgccgaac agtcagcagc
    37021 tggcggactt gattgagcag gagtcccagc cctccgcagc agactgtatg cgggccaatc
    37081 aaaccagccg ccaaatcggc cgcaaagcca atcagaaagg agcagacgat ggcctgccgg
    37141 gtgcgggtgc tgagggcata ataaaccagt acggtaataa ggagactggg acgaatgtac
    37201 catcctccgg tagagaagac agtcagcaga ttgcctgcct ccagcagggt gcacgcaaac
    37261 acgatgacaa aaaaagtcgg ccagcgcatg cagccaatta ctccttgcgc agtaagagca
    37321 tctcatcagc gacaataacg gcaacggaat ccagccgggt catgtcttcc gccggcatga
    37381 ccgtgatatc ccacagcagc ggatgcagtt catccggctg aacagaaata acctctccga
    37441 taaccatcgg gacttccagc ttgcccggcc ggctggctgc ataaaccgtg tctccttcgc
    37501 gaacatcctg ctggcggtca atcatggaaa tcctgcaaag ccctttggag ctgccgaaga
    37561 ggatagcccc gatatcttta tcggtgcctt cccgcctgat gcggacttca atgctctggt
    37621 tggcatctgt caaaagccgt acccgggcgg catgttccga tgtttcctcc acaatcccga
    37681 cgacgcagtg ggtctgttcc gataaaacat actgccccgg tcgaatcatt gctgtactgc
    37741 ctttgttgat aataatttcg tggctgtatg gactggccgc tccggtaact gccgccagca
    37801 ccagaccgct gcccggcagc ggcagggcgg tacggagata cgcaagagct tcgtagtccc
    37861 gatgaagctg catcagctgg gcatgaaggt tcttgtaatt tttccagagt ttctcgtatt
    37921 cgtaccggct gacattttcc tcctgtcccg gatgcagacg caatgcctcc agctgcacat
    37981 cccgacccag ccgaagcact ttttcaaagg cagaataaaa aagaagactg attttagacg
    38041 tcgtttgatg cggcagcagc agaagaatca aacctgctgt caggaaagag aagaataagc
    38101 ttgtgtttga aaagtgaata tgtttgccgg ccataacagg gattctcttg ggctattgcc
    38161 cctcaccata tctcgatcga gataaaccgg ccgcagtttt ttgcggactc agctccatcc
    38221 ttgatccagc gtgtctttcc agatttccag attttccaga tagacgctgg tgccgcgggc
    38281 aacacaagtc agcgggtctt cggcgtgtac caccttgagt ccggtggcat tggaaagcac
    38341 cttatccatt ccgcggagca gtgaaccgcc cccgcagatc gtaacgccgt tatcaatcag
    38401 gtcggcagcc agctcgggtt cggcacgctc caatgtgcgc atcactgttt caataatccc
    38461 ggcaataggt tctttgaatg cctcgcgtat ttcctcactg gtaatgacaa tcttgcgggg
    38521 cagacccgat atcgtatcgc ggccggctat ctccatcgtc atttcctgct ccagaggggc
    38581 cgccgaaccg atttgaatct tgaccatttc agcgcggggt tctccaatca gcaggttata
    38641 ggttcgcttg agatggttta tcagggcttc gtcaaagtta tcgccgccga tgcggatgga
    38701 ttcacaggtg ctgatatcgg acaaactgat aattgccact tcagtggttc cgccgccgat
    38761 atcaacaatc atcgatgcgg ttggttcggt tacaggcaag cccgccccga tgccggccgc
    38821 cataggttca tccaccaaaa acaccttgcg ggcgccggca cgctcggcgc tgtctataac
    38881 cgcgcgccgc tcgacagccg taatgccgct gggaaccgca atcaccacgc gcggacgaaa
    38941 caagccgccg cggccgtgca ctttccggat aaaatagctc agcattgcct cggtgatttc
    39001 gaaatcgctg atgacgccgt ctttgagggg gcgaatggca gtgatggaac ccggcgtttt
    39061 tcccagcatt tctttggcta caagacccac tgcttcgccg ttgttgagaa cctgattggt
    39121 tccccgccgc actgcaacga cggagggttc attcaggaca atcccttcat tgcgaacaca
    39181 caccagtgtg ttgcacgtgc ccaaatcaat ccccatgtct ttgctgaacc agccgagaat
    39241 tgtatccagt atcactgtga atcccttcaa attgggttgc cggcacgtta tgctccgctt
    39301 ttgaacattt caagaaccgc ttggacctgc ctgaattttc ctgccggcgt tcatgcttat
    39361 cgcggggccg aagcgatctt aaacaccgcg tcagagccgt aataggtgat gcatttgatt
    39421 gttataaaca ccacggcggc caacgctgct aagcatgcca gtgcaagtat cgcggtataa
    39481 acatcactgc ttggacttgt attttttttc gccatcgtat tcctgtctta caactgggtg
    39541 cttgctgtat cgccgacctg cggacgctgc tggaccaaat ccagcacgcc ggcactttga
    39601 ttgatatcga catcggttat gatgacatca cacaaaaacc gatcgccgcg ggtaatatga
    39661 aacaccatct tttccttaac cccgtctgcc gaaccaaccg atagggtgat cagattctct
    39721 tgcacctcgg tgaccagacc tttaatctcg tatgccgtct tctgcttgac aaaaaaagca
    39781 gctgttttcg ccagcggagt aaccggtatg gcctcacgaa tgcctatgcc cacggaggaa
    39841 atctggtttt ccagttcctg tttctgttcc aaaagacgcc gccgttctgc ctccatcgac
    39901 tgaagctgaa cgattttttc gtgcaaatag gctgtaatct gattcaattc cttctggtct
    39961 ttgatgcctt gtgcacgggc ctgatccagc tgctgttggg tttggctcag cgtggcctgc
    40021 aggttgcgaa tgctttgttc aaagcctgtc agcaggcctt tccagctgtc ggcctgcgcc
    40081 tgatactgct gagccagccg ctcggctttt cgaaggtcag ccgccagctg atttttttcc
    40141 gcttccagat tctggatatc ctgctgcagc ttaagctcca gctcggaaca cttctttgtt
    40201 aaagtgtcgt attgttcgtt cttggtggcc agttcgccct gcagcacgat attcatgctt
    40261 ttttgttctt cgtaccagcc cttgtaattt gcagaattgc cgacaaacgt tacaaccatg
    40321 ccgcacagga gaatggagaa aatggacagc aaaacggcta aaatttttgt tagaatactc
    40381 aagttacatc cctttcacca caaaggttta aataataaca tcacataccc atccatacat
    40441 tcggtagcgg cacaacgcac gcccccgaca cacaatgcct gaattgtacg gattttacga
    40501 caggcctttt aacaggtcaa gcgtttttta tctctattat atacttattt ttgccggttt
    40561 ttcgccccta ttttgcctga agcagcacgg cctgtgcagc aataagctgc aggtcgcgcg
    40621 agggagtttc tatgatttgg ggcagaagac tgttcagccg gctgctggcc aaatacccga
    40681 tggcgcgcac ggccattccg ttggccatat ccatcttatt caagtccggc tgtgtctgga
    40741 aatattcata aacctgcgtt tctccccgtt tgtcgcccag ccgtgccagt tgttcggcag
    40801 cagtcagacg aacctccggc acatcgtcct gcagcattgt aacaattgcc ccgcgcgatt
    40861 cgggagtatt cagcgccccc atcccgcgaa tccccataac gcggtcatcg gcaaacttgc
    40921 tgatcagcag tgcccatagt ttactgcggt acatccgctc atctcccaga cgggcaatag
    40981 attcaaccgc ctgcatcctt actttgtcgg tcgactcatt gttgttgagg acttcataca
    41041 gcagggcaag atcatcccga ttgccgagtt tgcccaaaag caggacggcg ttggcccgga
    41101 cagtcgggtc agtgctgacg gcagcggtcc gtatcagctc tcggtatttg agctgattca
    41161 tcttggccag cgcgtaggct gctcctatct gcacattcat atccgggtca ttcagcatgc
    41221 gtgtgagtac attttcgcag ctgaagcatt gcatatcacc caatccggtt gctgcagcaa
    41281 aacgcaccgc aaccgaacga tctgttgtca gtttgactac cagcggcagc atctgtttct
    41341 gtctggtttc agcaacgacc tcaatcgcat ggctgcgaag ataagcattg tcgctgttca
    41401 gtgccgcctc gagaatcacc agagcacggg ctttgagaga ttggagtttt gcaggtgttt
    41461 ccagaccggc ggccggctgc ggtgccggcg gtgccgaatt tgtgcatcct gcaaatgcta
    41521 aaacaagcgg caatgaaaat aacaggactg tcttcatctt tatttgtctt tccttaaatg
    41581 cttcctgcat acatcatata tcgaccagat cagaattata ctccagcccg ctccaaaaac
    41641 aacatccagc caacgaccgt gccggctgaa aaatgtaatg cggctgtcaa tgggaactcg
    41701 atcgacaaac caccccctga ctccttgtct gtccatggct tcagcaggga gagtgccggc
    41761 aagaaagccg ttgcggatgc ggcctgtcgg ctctatcaga cagctgatgc cggtgtttac
    41821 gctgcgcacg atgctgatgc ggttctcgac gcagcggaat acactgattg cggtacgctg
    41881 ggccagctcg gccatcggat agacccgacc atttttaaat cgaacatacc agccgtcatt
    41941 gctcaggtta accagccagt cggccttttt tttgccgtca ggcccgacag cgagcttgcg
    42001 ggtaacggtt gggtctgtat cttcataaca aatcaggacc ccgaaacgat agacccggct
    42061 gttgtctttt atctcaaatg ttgtaaactc tttgcccgga gtcaggttat agtcatagtc
    42121 atagggactc agccataaaa tagctttata gagccatggt attgtgtccc gaaaagggat
    42181 atattctcca aacggcacaa ggtggatttt atcataccgt tgggggtcag ccgtgccgtc
    42241 agggcgataa agataggccg agttatattg gtcagtgatt gcataacgtc cgttttgtat
    42301 gccgatggct gccccatggg ctccggccag tatatacgcc ctgtctttgc agtgctcgat
    42361 aatctgccgg tgaaagcggg gaggatcaga attggccaca cacagatcca gatactcctg
    42421 attcagggga gccagaacca ttgtttccgg ccaagccgcc atgacagctc ctgctttgat
    42481 gcattcattg ctgtcggcga tcaagtcgtc taaaatcttt tgtccgttgt ccatttcttc
    42541 tttgatccac gccggcacat tcggctgcac catcccgaca agcggtcccc ggctcagata
    42601 cgccggcgtt tgacggatgc gatgctgccc gtaagccagc gtcagcatta taagaagaac
    42661 cgctgcagca gcactccaaa aggttcgcat ggccgcagtc cggccgctcc tccggtctat
    42721 aatccattgg gcaataacac cattaaccag tgcaattaat gccgaaagcc ccaaggctcc
    42781 aaagatgtcg catatctgaa tgatggacag attgcgatat tggctgtgag caaggtaata
    42841 ccagcaaaag ccggtatata atatcccttg ccatgcctcc gccccgacga atatcaccgg
    42901 cgccatcagc gccagcggcc agttctttcg ctgcgcaaac cgcaccaacc acgccaatac
    42961 cggccagtac agtccctgca ttacagcaaa ggcaagatag ccggcaatgg tgatgatata
    43021 cagccagtat aagttgccca cccaatagca aagaccgata aaataggcac ttaaaagcaa
    43081 tctgcctgtc tgtaactttg atttgcacgc cagcatgaac ggcacccatg caagccatgc
    43141 cagaaaatgc agattccacg gcgcctgaat gactgtcagc agaatgcctg aagcagcaaa
    43201 ccccaaaaca gcattgaaaa ggggatggtt tattttttgc tggcccatgt cgataagtct
    43261 ttaagggctt ctttgattag ctggacatca tacattgttg ggctgttcag ccgcaatcgg
    43321 agctggttca taaataaagt cagcattttg ctgttatgaa taagtgtctc cgctgcaata
    43381 cccatctcgg caaacagccg cttgaattca aaaaattctg ttgtgcttaa ttgcccattt
    43441 tggtagctgt gctcagtttt ttcgatgtct gccagacgcc ggcaggtttt ttggacgtag
    43501 tcttcagaaa tgtcaatgcc gcagtattgc cgcccgagct gaaatgccgc tgcagcggtt
    43561 gttcctgagc cgttaaacgg atcaagtacc aaatcgcctg tgcgggagct tgcggcgata
    43621 atccgtccca gcagcagttc aggcatctgg caggggtgcc agccctgcct ttctctgaat
    43681 gtgccgcata cccgagaata gctgtcccag acatcgttgg ggattttacc tgcggcgttg
    43741 gaacgcctgt cattatagat tagctgtctg tccgaaggca gccgaactgc caaatcattg
    43801 aagataaaat tttttggatc gttgacaaaa taaagaatat gcgtatggga gcgggcaaac
    43861 ttgttttttg tctgctggcc gaaggtgtag tgccagataa tccagttgcg gcagtgcagc
    43921 cccagctgat cgccgataat ccgcacatgc gcggcatagt catccccgat ggcgatataa
    43981 aacgacccat gcggttttaa gacatgcacg cacgccgcca tccagtcccg cgtccaagcc
    44041 aagtagttat tcgtctcgac cttgtcgtgg tacttatcgt acggatagcc gatattaaac
    44101 ggcgggtctg caaacaccag atctgcaaac ggctccgtga cgccgttaag caccgtaatg
    44161 cagtcgccgc agattatcgt attgtgaagt ttctgcataa agggtgcgat tactgctgtt
    44221 taaccagtcg ggctgcaaat ccgccgccgc tggcaagttc gattgtcagc gtgtcatttt
    44281 tggtaaccgt tttggttatt ttgcggtagt cttctgcaaa gcggtctgca ttgatgccgt
    44341 cctgaaacag agtcattgca tacgttcctt cgtcgaggaa agataaatcg aacaccgccg
    44401 ttctcttgtc ttcgttggtc atcccgccca gataccactc attgcctttt ttgcgggcca
    44461 tatagaggta tttggcgatt tttgctgcca tcacgcgcgt atcgtcccaa accgtcggca
    44521 ccggtttgag aaactccatg cattccggct cgcgcaggta gttgctcgga ctgtcgcaga
    44581 gcatctgcag gggcgattca taaataacat acatcgccaa ttgacggcag cgcgtcccta
    44641 tcacgccggg acgattaaag accggattaa actggtcggg ctggaaattg cgcattgccc
    44701 ccggcgtaaa atccattggt ccggcaacca tgcgtataaa aggcagagtt acagtgtgcg
    44761 aaggggtaat gtttttggac catttgctgt gttcattgcc ttggacgcct tcccgggtta
    44821 ttacattggg ataccgccgc cgcagaccgt cgggtttata agcgccgtga aaatcaacca
    44881 gcagatgccg cttggccgct tccgctgcaa ctttctcata gtactgcacc attttctggt
    44941 catcccgctg cataaaatcc accttcagac ccgcaacctg ccattttgaa aacagctcca
    45001 ttgccggctc aaattgctca tcgagcgttt tccaagttgt ccagagaata atgccgacat
    45061 ttttttgctt tgcataccgc agcagttccg gcatatcaat gtccggtact gtcttcagca
    45121 gattgcccag agggtaccac ccctcgtcca gaatgatgta ttcaatgccg tattgagaag
    45181 caaaatcaat aaagtatttg taggtggctg tattgactcc ggcgcgaaaa tcgactccat
    45241 aaacattgtt tgcattccac cagtcccatg ccaccttgcc cggacgaatc caggatgtat
    45301 cttccagcgt gcaatccgac gccagcttat aaaccgtttg gttcagcacc aaatcggcat
    45361 ctgtcggagc gacaataacg acacgccatg gatacaatcg ggtcccgctg gttttagcca
    45421 agtaatccgc ccgcttgaca acacgaacgt ctctgtcccc ttgtggcctt tcttcaagcg
    45481 gataattcgg aaagatggct ttaagggttg tggttccctg tgcttgcaga tagcagcccg
    45541 ggtagtcgat cacatcggac tctgttaagg cgattttcgg cccgccggga atatcaacaa
    45601 ccgccgggat tgagcagaac gctccttttg tcaattggga cagcggctta tttatataag
    45661 agcgttcctg atgggtgtag aaactgtctt cctgaggaaa gaatatcgga taatcttctg
    45721 aaaatgcaaa cgcgatttgt tctgcgacaa cctcaacctc tccgggcagt ttggtctgga
    45781 tgcggtaggc aaaaccgtcg ttataaaccc ggcaaataag cgaaaatgag ctgttgacag
    45841 ggaaggtaac agttgtgtat tggtcttgta taatccgttt tttttccggc accacggggg
    45901 tgatggtttc atcgactagc gtttgactgc tctcgggcag aacgacattt ttgcccagtt
    45961 cggtcagctg cccgtcagct gttcggatgg ttaatcctat cggacagttt tgcatcagca
    46021 ctgttccatc ataagccaga gaataaccga gcatcgggct gtcagtaaaa atgaccttga
    46081 gtttcccatc gggggatttg atttctgccg tgccggcatg aacggatacg gcacttatca
    46141 acaatgtcaa tccgaccgca agacgcgtta agtgtcccat aatgtctccc ttcgaacaat
    46201 ttttagctga caaatttccc cggatttccg gactgccgca attgtatgga taagacggtt
    46261 tttagtaaag tacaaattgt caggttgaca gttcgcctgt tttgacctat tatagaaagg
    46321 cagtggtttt ttgaccaaaa acactgaaaa atcgctgcga agggaagaaa ttgtgaaaat
    46381 aattgcggtt attcccgccc ggtatgcatc cagccgtttt cccggcaagg ttctggccaa
    46441 agagaccggt aaatatctta tccagcacac ctacgagcgg gcgttgtgtg caaagtgcat
    46501 cagtcaggtg ctcgtagccg ctgacgacca gcgcgtggca gacgcctgcc ggcagttcgg
    46561 ggcgccgtgt gtgatgacct ctgtaagcca tcccagcggt accgaccgca ttgccgaagc
    46621 ggctgccggt gtcgatgcgg atattgtagt caatctgcag gccgacgagc cggaaattgc
    46681 cccggcctat attgaccgcg tggcgatgct gctggttgag cacccgcagg ccgcaatggc
    46741 tacgctgctg gcgccgtttg aaagtattgc tgatattgcc aatccgaata ttgtcaaatg
    46801 cattacagac aactccggaa gggcgattta tttttcacgg tcagttattc cgtatgaccg
    46861 tgccgccggc ggcataggcc gcatcgatgc ctaccatcgg catcttggca tttatgccta
    46921 ccggaaagag tttttaatga cctttactgc tctccagccg ggttttctcg aacagcacga
    46981 gaaactcgaa cagctgcggg ctttggagaa cggctatgcc attttgaccg cctgcgtcga
    47041 aaaaacgtgg gatggcattg atacgcccga gcagtatcgg gcttttgttg agcgaatcag
    47101 gcagatcaca caataacatt tggaagataa tatgtcagac agcatggatt tattggccaa
    47161 aatcagcggc acctcgactg ataacgaata tttcctcccg ctgccgccgg gctatcagaa
    47221 agggaaaacc cgctatgtga tagtcatggg cacggtgatg agcgggctgg gcaaggggat
    47281 tttttcttcc tgtctggcca agctgctgca ggataaagga ctgacggtgg ctcccatcaa
    47341 gctggagggc tatctgaaca ttgattcggg cacgctcaat ccatttcgtc acggcgaggt
    47401 ttttgtcctc gatgacggaa tggaatgcga tatggatctg gggacctatg aacggatgct
    47461 ggaccagaac ctctcacgcc agaatttctc aaccagcggc cagatttaca gttcggttct
    47521 ggaaaaagag cggcacggcg attatctcgg ccgcgatgtc cagatgattc cccatgttac
    47581 cggcgaggtc aaaatgaagc tgcgccagct ggcgatgaaa accaatgccg atgtggtctt
    47641 cgtggaaatt ggaggtacag tcggcgacct tgaaaatgcc ttctatatcg aagccatgcg
    47701 cgaactggct tatgaggaag gtcccaacag ctgctgtttc gtggcgctga catatatcct
    47761 tgaacccaaa gccctcggcg aacagaaatc caaagccgcc cagctgggca tcaagcagct
    47821 gatgcagatg ggcattcagc cggatattat cgcctgccgt gccgagaagc ccgtcagcga
    47881 tcaggcacgc cagaagatga gtgtgttcgg caatgtgccg ttcgaacggg tcttcagcat
    47941 gcatgattca gccagcattt acgcaatccc ttccatgctg cgggactgcg gcatcgattt
    48001 tgaagtgctc cgcatgctca agattgaaga ccgcatccag ctgcgacacg agcgcaaaga
    48061 gtgggcacgc tggtgcgatt ttaccgacaa aatcggcaaa gaagcgtatg acattaccat
    48121 cggcattaca ggcaaatata cctcggtccg cgacagttat gcttcggttc tccatgcggt
    48181 cgagcatgcc ggtattcatc tgggtatccg ggtcaatatc aagtggatag agaccaccga
    48241 tttaaccgat gacaatgccg ctgcctgtct ggcagatgtg gacggcatta ttgttcccgg
    48301 gggcttcggc acccgcggct gggagggcaa gattgcctgt gtgcactatg cccgacagaa
    48361 caatgtgccg tatctgggac tctgtctggg ctttcaggcc gccgtgattg aatttgcccg
    48421 caatgtgtgc ggcttggccg aagccaattc caccgagatt gatccccata tcgggcagcc
    48481 ggttatcagt atcctgcccg aacagaaaaa gattgaagga ctcggcggca acatgcgtct
    48541 gggagggcgg gatgtagaaa tcaaaaaaga tacaatggct tggaagctct tcggtaaaac
    48601 gtccatcgtg cggctgcggt ttcgtcaccg ctacgaagtc gatcctgcct atgtcgggct
    48661 gctggaagaa aagggactcg tcttttccgg caaagccccc aaccagccga ttatgcagat
    48721 ccttgagctg ccgtcgcagc tgttttttat ggggacgcag gcacatccgg aactgaccag
    48781 ccggccgatg cgtccctcgc cgttctttgt cggcttggtt gccgctgccg gcaaacgccg
    48841 ctacccgaat gccggcttgg ataatccgct ggaagcggtc gaaaaagtca ggggatttta
    48901 gcgccccttg tcggatggtc tgcagtgcat gaggcatcgg aaagaaatgt gcatgtttga
    48961 cctgcatcgg tcggaaaggg cggttctatg aagtacggta tggcagcagt tgtgttgtgt
    49021 gttgcggtct ttgcaggata ttgcaccagc gcatggtcca aggcgtctgt accggtcctt
    49081 tacatcgtcg gcgattcgac tgccgccaac ggtcctgata agggctgggg cagccatctg
    49141 cagaagtttt ttgaccccaa tcagctcaac gtggtcaatg cggcccgtgc cggccgaagc
    49201 agccgcacat acattacaga gggtttttgg gatgatgttg tcaaaaacct taagggaacc
    49261 tctaataatt tgatttttga ggatcaaaat ctcagttaac gttttt
//
LOCUS       DAOTBO010000014        45136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_685_length_45136_cov_5.169140, whole genome shotgun sequence.
ACCESSION   DAOTBO010000014 DAOTBO010000000
VERSION     DAOTBO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 45136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45136
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_45136_cov_5.169140"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLE88_03130"
     CDS             complement(<1..340)
                     /locus_tag="PLE88_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75492.1"
                     /translation="MPIRHEDKEFIQSLSLFGALLGACLGAFAVVHLSDRARQDYARQ
                     SQTYQEQAALLKLNQTLFKSTVKPLVDEELIDRAEEHITGPEPAAAPVKGFWLTIPRW
                     GYWGLCGVSGA"
     gene            486..1508
                     /locus_tag="PLE88_03135"
     CDS             486..1508
                     /locus_tag="PLE88_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOM75493.1"
                     /translation="MDKKSVSFFIVGILAGILISVGGFTWYVRSAAVGGGQRNEVLVL
                     KLGHGLDPSHPVHKGMVYMAERLKEKSGGTIELQIFPGGILGSETECIELLKNGALAV
                     TKVSTAPLEGFLPEMGVFSLPYVFRNEEHYWKVLTSPLGRKLLQMGEPAGLHGLCYYD
                     SGSRNFYTTTKPILTPDDLKGLKIRVQNSKTSMDMIEVLGAAATPISWGELYTALQQK
                     MVDGAENNPPSFVTSRHFEVCKYFSFNEHTRIPDILLMSKKIWDTLTPAQQQWIQEAA
                     DESSLYQRQLWKTMTAECIETMKKEGVQLFYPDTKPFEEKVQPLLRQYEGTPIGDIVR
                     QIREIQ"
     gene            1508..2026
                     /locus_tag="PLE88_03140"
     CDS             1508..2026
                     /locus_tag="PLE88_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOM75494.1"
                     /translation="MGTWLRRIKKGLQTLLEWGVIVIMAILVLDVLWGVFSRYVLKAQ
                     SRWTEELAIFLLVWVSLLGASVAYASKSHLGVDYFVGKLDRQAQRLAELLVHLAVAVF
                     AASAFVAGGWALVSKTLAAGQISPALGVKMGYVYLAVPISGVFFLIFAAENIAELFAG
                     KPTASAMQSKEN"
     gene            2030..3325
                     /locus_tag="PLE88_03145"
     CDS             2030..3325
                     /locus_tag="PLE88_03145"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOM75495.1"
                     /translation="METQVLILVISFTVLLLMNVPIAVCIGLSTLLTFYSIGDIPTAY
                     TVAQRISMGIASFPLLAIPFFILSGILMGEGGMARRLINFAGSVVGWLTGGLGYVNTL
                     TCMMFGSISGSAAAAVSSVGGFMIPEMNKQGYDKAFNVAVTTTAATTGLLIPPSNVMI
                     VYAVVAGNVSVAAMFMAGVLPGILVWLAIMLVSGIISHIRGYGASDTVSVRRMLKTFL
                     EALPSLLLVVIVLGGILGGIFSATEASAIAVAYSFLLAVLFYREVKLKDLPRILLECG
                     RTTAVVMLLVGCSQAMSWILAYENIPQSVSASLLKISSNPVVILMIINVLLLVVGTFM
                     DMTPAVLIFTPIFLPVVVGLGMNPVHFGILLIANLCIGLCTPPVGTCLFIGCGVGKIS
                     IAKVLPSMIPFFIAMIGALMVITYWPWLSLWLPGIMDLL"
     gene            3360..4787
                     /gene="pyk"
                     /locus_tag="PLE88_03150"
     CDS             3360..4787
                     /gene="pyk"
                     /locus_tag="PLE88_03150"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOM75496.1"
                     /translation="MTTASKTSIVATLGPASSDAAVLESLVRQGVSVLRLNFSHGTFE
                     THAQTLAHISRIRKSLPYEIAVMGDLCGPKIRLGQVEPQTVLAQNAEVMLSAGTAVGT
                     AAQLFTDFEPLVRDVQPGQRILIDDGRLLLQALEKTAAGIRCRVAVGGPVSSRKGINL
                     PDTRLAIQAITEKDWQYADWAAENQLDYLALSFVQRADEIVRLKAYLASKGSGIRVIS
                     KIEKPLAVENIEAIIRASDAVLIARGDMGVEMDLAQVPLVQKKITALCRRLAKPVIVA
                     TQVLQSMIENASPTRAEATDIANAVMDGADAVMLSGETAVGRYPLAAAAALSHICRTT
                     EQFLEAASSVRAPMETPPEQIEKAAIARAVAQMLDEVKCACIVVWTKTGQMAHLLSKA
                     HPDVPIVSLSANRLLARQLALCRGVVSIYQPAVSAYEEWIGLVHRLLLAGSWAAAGDK
                     VLLIPPLELLTAQTSGALILHTLAK"
     gene            4940..7033
                     /locus_tag="PLE88_03155"
     CDS             4940..7033
                     /locus_tag="PLE88_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75497.1"
                     /translation="MKKIYFLLFLAAGLTCAAAIDRFSLVNRHNITLTRFDAFSPLSV
                     GNGHFTFTADITGLQTFPEAYAKGIPLTTMAEWGWHSFANTEGYKLEDTFVQVDTYGR
                     PVPYNSNQKCPAADYLRANPHQITLGLAGLVLTRSDGSPARLEDIGAIEQQLNLWQGL
                     LTSRFELEGQPVAVETCVHPQLDMAAVRIRSPLIAQKRLAVVLRFPYAAGTWGSDPAD
                     WSSPDKHQTLVAVRSEDRAVFERIMDDKHYYCYTAFSNNTQLQQTGPHTYLFVPPAGD
                     AFDFSVLFDPAAGIPQMPDAAATQAVCAEHWKAFWTSGGAIDLSESKDPRWKELERRI
                     VLSQYLTAIQSVQKYPPQETGLTCSSWFGKFHLEMHWWHSVHFALWGRLEMFESALDW
                     YREILPAARQIAQRQGYKGVRWPKMTDPSGQDSPSGIGPFLIWQQPHPIYYAELVYRQ
                     KPTAQTLERFKDIVFETAEFMADFAHWDPVSESFVLGPPVIPAQENYKYDKTRNPTYE
                     LCYWYWGLQTAQKWRSRLGLDPQPQWQHVLNNIALLPMRDGIYCAVETEPFTNLTDHP
                     SMLAALGVLPPTPAVDAAVMKKTAQWVYDNWQWADTWGWDYPMLAMTAARVGLPELAV
                     EAFFIDSKKNLYWPNGHNYQRDNLPLYLPGNGGLLAAVAMMAAGWDGCPDRPAPGFPD
                     NGQWTVKWENLQKMP"
     gene            complement(7188..7904)
                     /gene="hisA"
                     /locus_tag="PLE88_03160"
     CDS             complement(7188..7904)
                     /gene="hisA"
                     /locus_tag="PLE88_03160"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOM75498.1"
                     /translation="MDILPAIDIIGGRCVRLIQGCYDRQITYRDDPAAQAAEFYQAGA
                     VWLHLVDLDGAKAGRPVNAETVAHIAAQVPMKIELGGGIRTEEAIVQMLEAGVTRLIL
                     GSSAVKQFDWFCRMAEKYPHKLVLGLDARGSTLAAEGWLEEAAQTVLEFAKKAADLPL
                     AAIIYTDISKDGMLAGPNIERTRQLVEAVELPVVAAGGVTTVEDVKALKRAGVAGAII
                     GRALYEGTIDLKAALKAAYE"
     gene            complement(7909..8346)
                     /locus_tag="PLE88_03165"
     CDS             complement(7909..8346)
                     /locus_tag="PLE88_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75499.1"
                     /translation="MAKQLTIFLENRPGRIKSISDVLLENSLNIWAFTIQDRGEFGLM
                     KMIVDKPEKAQLILADRGFACVLKEVLAVVAEQDQPGNLDKLTTALLERGVNIKDAYG
                     FVSLKDRQGICFMEFENPEQVNLEEAIAAYGFKVVSGQQLYEL"
     gene            complement(8364..9674)
                     /locus_tag="PLE88_03170"
     CDS             complement(8364..9674)
                     /locus_tag="PLE88_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOM75500.1"
                     /translation="MEMTYWNRQAETMERAALEQLQIQRMRSIIAQALKTPFYQKRLS
                     RAGITSPDQFRSLADMQRLPFTTKDDLRQNYPVGLLAVDMDEVVRIHSSSGTTGIPTV
                     IYLTQEDLNAWTDLLARSITATGCTRKDVFQNMMSYGLFTGGLGLHYGAERVGMTVIP
                     IGGGNTQRQVQVMKDFKTTVLHITPSYLLHIHSRLDEFGVKPGDLHLKKAFLGAEPYS
                     ENTRQKLQELFGIDAYNSYGLSEMNGPGVAFECVYKQDMHLWEDAYILEIIDPATGQV
                     LPDGQLGEVVLTNLVRMAMPLMRYRTRDLAFVHPEPCPCGRTHRRLSRIQGRTDDMLI
                     INGVNVFPSQIEEVIMSIPEVGTNYMIHLTRAGALDKLTVKVEIYSKMFTGDLNALEA
                     LKNKIRDRLRASITINPHIELHEPGALPAFEGKAKRVVDQREKL"
     gene            complement(9709..10140)
                     /locus_tag="PLE88_03175"
     CDS             complement(9709..10140)
                     /locus_tag="PLE88_03175"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOM75501.1"
                     /translation="MKITQISVFLENRKGRLYEICSMLGKAGINIRALTIAETESFGV
                     LRMVVDKPAEAVELFRRNSVTANQTEVVAVEVPDKPGGLAEVLKVLSDHNVNVEYMYG
                     FIEKFSENALLVFRFDNPEYAHQVLSQNHIKVVGQRDIKGL"
     gene            complement(10159..11460)
                     /locus_tag="PLE88_03180"
     CDS             complement(10159..11460)
                     /locus_tag="PLE88_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOM75502.1"
                     /translation="MIWNEPVETMPLEDLKNLQSERLVKLCRYVYEKCPVYRRKFDDH
                     GISPSDIHSIDDITKLPFTTKLDMRDNYPFGLFSVPRTDLVEIHVSSGTTGNPTLVGY
                     TRDDIELWSEVMARTLCCAGARTDDTVQIAYGYGLFTGGLGFHYGALKMGLTIIPTSS
                     GQTKRQLKIMQDFKPRILCCTPSYCLYMAEEAREMGMDPRAASWNIGIFGAEPWSESM
                     RTEIEQTWNMLATDVYGLSEIIGPGVSQECHYKDGLHIYSDVFYPEIIDPESGRPVGE
                     GQDGELVITTLTKQGIPLIRYRTRDIVSMTTQPCRCGRTSPRTSKIKGRTDDMIVVRG
                     INVFPSQIEEVLLGIEGTHPHYQIVIDRQAHKLDEVEIMVEVDEKIFSDEIKRMHALE
                     QKIKKEIEAVLSIGVTVKLVEPKTIQRSEGKAKRVIDKRQL"
     gene            complement(11542..12027)
                     /locus_tag="PLE88_03185"
     CDS             complement(11542..12027)
                     /locus_tag="PLE88_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOM75503.1"
                     /translation="MKKQDCYNITFGGIGGQGIIKASELLGWAAMYDGWHVKKSEVHG
                     MSQRGGSVESHVRFGRKIYSPLIGPGQADFLLCFHLDEHPRLVMFLKPGGTDLAGYLQ
                     QAEAMVENPKHVNTALMGVLSVFLPISEAGWQKAMETVFSPKIRDENRQVFQRAKALV
                     K"
     gene            complement(12044..13600)
                     /locus_tag="PLE88_03190"
     CDS             complement(12044..13600)
                     /locus_tag="PLE88_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOM75504.1"
                     /translation="MAKKTSVQKAFLSGNEALARGAWEAGLTVACAYPGTPSTEILES
                     LALYPELDVQWSVNEKAAYEVAYSAAVGGVRSLFACKHVGLNVAMDPLMTSSYTGVNA
                     GFVAVVCDDPGMHSSQNEQDTRWAGIYAKLPILEPASPAEAKQFVAEAFAISEKYDTP
                     VILRMTTRVSHSKEDVLLGQRRPAEKRPLKPDIQKYVMVPAFARQRHALVEQRQLKLQ
                     KLSERSRLNKMELNDLSVGFIAGGVSYYYLKEARPNASFLKLGFLYPFCDAKIKAFAG
                     KVKKLVVVEELDPFIEDHIRQLGIKKAVFRHPSFRLGELGPGVIEDVIAGRPKKAVKL
                     PPARPPRLCAGCPHWATFSVLKKLGLFVAGDIGCYTLGTLPPTSALHTCLCMGAGVTF
                     NEGLRRAHPDGKIVGVIGDSTFIHAGITGLINAAYNKAKGIIFILDNSTTAMTGGQNH
                     PGTGLTIRGQPTKTLDLKALCLACGADNVDVMDPAGNVGQLEELVRRRLSEDALTVII
                     AKHPCKLIKR"
     gene            complement(13807..15282)
                     /locus_tag="PLE88_03195"
     CDS             complement(13807..15282)
                     /locus_tag="PLE88_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75505.1"
                     /translation="MKKGKLFAGLVLTVFVAAAAVAMAAQTAEPAKHWHEIDTDWMDK
                     FHHPMEGVEMGLDIRLREVYTSNLFGLNETWGDGIGQDWNNFHWQRYRTRLSAKWAVS
                     DEVTFNTRLTWEFWGHCAPDHTFTSFFTEKNYDFDEALFDIMNLQFRNAFDLPLTLTV
                     GRQEIVLGTGWLVLDGTPGDGSRTMFFDAIRAQYDLTDTSKLDLIYIQQYDDEEQYIK
                     PFNHHAVQDRRHLTQNNDERGFIAYWTNKLSDTLSNELYYIYKNDDSSDWANHYKGPS
                     YGVDADIHTVGTRFFGKLDDHWSYSAEVAKQWGDREGDTLQALGGNSKLMYAFNDSWN
                     NELHVGYEYLSGDDPETSADEQFDSLWGDWPQFQRGRDLQSYLWTFEGDGIGNVTNLH
                     RLGFGHSFKPTAVWTIATDYNLMWADENTFTGARGPQNALTFDGSNNFRGQMISAVAT
                     YSCCKNFRTLFEIDYFIPGSYYEEPSQDDAYFARIGVEWTF"
     gene            complement(15332..17137)
                     /locus_tag="PLE88_03200"
     CDS             complement(15332..17137)
                     /locus_tag="PLE88_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOM75506.1"
                     /translation="MLAQAVGLWDIIVVLIYLLLVVYLGILGYMRTKSAADYLIAGRQ
                     VHPFVMAMSYGATFISTSAIVGFGGVAGLFGMSLLWLTFCNIFVGIFLAFVFIAPRTR
                     MMGHHLDAHTFPEFLGRRFESRFIQVFAGTVIFLFIPLYAAAVLIGGSVFVAKSFNME
                     YPTALLLFSVIIAAYVIVGGLKGVMYSDALQGSIMFIGMFALLIYGYVKVGGVIEGHQ
                     KLSALAPLVPGKLSAIGHRGWTAMPAFGWGDRQYDLWWTVIGTIVLGVGIGVLAQPQL
                     VVRFMTVKSRRELNRAVLIGGVFILVMTGVAFTVGALSNAYFSEHGAPLSGRVVKMLD
                     AEKGLGVLQIMKKGDTGVWADVPEKIAPVKLFGQAVDRTEIDGQPVEIRQGRSISIVY
                     AGGTADEIIPTYIVSAMPKWFGLLFLLTLLSAAMSTLSSQFHTVGTSIGRDVYEQITG
                     AHGASTNITRIGIIIGIIYAVIISFYARESTFIARATSIFFGLCASAFLPSLLGAIYF
                     RRMTKAGAIWSMLIGFIITAFWLLLVKAKEAESMRLVQWITGGKTSILADCPNWDVVD
                     PLLVALPVSFLAAIVVSALTQPPSKEHLDKCFKGI"
     gene            complement(17180..17350)
                     /locus_tag="PLE88_03205"
     CDS             complement(17180..17350)
                     /locus_tag="PLE88_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75507.1"
                     /translation="MLGIEDKGVLAAYLLCIASTLLCVIWGLINWNRGEESVHPDDIK
                     WAAEEKKTEQEL"
     gene            complement(17564..18364)
                     /locus_tag="PLE88_03210"
     CDS             complement(17564..18364)
                     /locus_tag="PLE88_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOM75508.1"
                     /translation="MTDKIIDFHTHAFPDALADRAMAQLKAECAEVRSFLDGKLASLL
                     KSMDANGIEKSVLCCIATRPVQFESIFNWCCQIASDRIVPFPSVHPQDPLAVERVAQI
                     AQAGFRGLKLHPYYQSFVLDDSALFPFWQAVCDSRLLVTVHTGYDIAFERIERASPRQ
                     ILTLLERFPSLQLIATHLGGWQQWEEVENLLLGKPIYMEVSWSIEYLGASRTKQMLMR
                     HSEDHILFGTDSPWTDQSETMESYKKLNLPPHIAEKFFYKNAARLLEE"
     gene            complement(18452..18655)
                     /locus_tag="PLE88_03215"
     CDS             complement(18452..18655)
                     /locus_tag="PLE88_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOM75509.1"
                     /translation="MAVGTVKWFDEKKGFGFIIPEDGGADLFVHHSNIIAEGFRTLKD
                     GQKVQFEPAQGKKGMEATQVKPC"
     gene            complement(18868..19299)
                     /gene="arfB"
                     /locus_tag="PLE88_03220"
     CDS             complement(18868..19299)
                     /gene="arfB"
                     /locus_tag="PLE88_03220"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOM75510.1"
                     /translation="MEDLAVNPQLIIPARCLRIFYTRSSGPGGQNVNKLNTRVQIFLD
                     IPACACLSDNQKNRLYTMWKGRIDKKGVLEVSCSQYRSQMENRRAAMKKIAQMIASAL
                     KPVRVRKKTAVPRQAILSRLHNKKERSELKKMRSKTGWDIQ"
     gene            complement(19342..20028)
                     /locus_tag="PLE88_03225"
     CDS             complement(19342..20028)
                     /locus_tag="PLE88_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM75511.1"
                     /translation="MNAKVLVVDDQRDLLELLSIALSQEGYIVRTAASGAEALSMIAA
                     EPPDLILLDIVLGDISGIKLTNRLKNDSKTARIPIILLTAKDSETDIIVGLSVGADDY
                     ITKPFSTKVLLARMDAVLRRVYPADTAAVRENLQAGPVRVFPEQRQVFVEGNPVDLTP
                     AEFNILLALLKAGGNILSREQLLKQAAPAEQNANARIVDVHVAALRKKLGKSRTLIKT
                     IHGKGYRFIL"
     gene            complement(20148..21605)
                     /gene="gatB"
                     /locus_tag="PLE88_03230"
     CDS             complement(20148..21605)
                     /gene="gatB"
                     /locus_tag="PLE88_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOM75512.1"
                     /translation="MEYKIIVGLEIHVHLATQTKMFCGCRLAYGDAANTHVCPVCLGL
                     PGALPVMNKQALEFAILTGLALNCRIARFTKWDRKSYYYPDLPKNYQISQYDLPFAQH
                     GYIEIPAADGQPKKIRIRRVHLEEDAGKNVHSFGNFSAIDLNRAGAPLLEIVTEPDMN
                     SPEEVGALARELQRIVRFLGVSEADMQKGHMRFEPNVNLHITRNGQLFKTPISEIKNL
                     NSFRAIERSIACQVQRQLEDFLETGRTLQMGNKSTWGWDDERQTTVLQREKEEAHDYR
                     YFPDPDLVPVEVDQAWLTAIQARLCELPLQMQMRFIQAYGLSPYDAAVLTADRATAEF
                     FDAAAALQADPKRICNLLTQTGLKLANERGIRVDQLGLSAQQLAQLAQMTDAGLISAT
                     SAAVIFEEIAAHGGDPVQIAEKRNLLQKSDAGEIETLVDAVLAENVQAVADARSNPKK
                     AKKALGFLMGQVIQKSKGQANPKIVSEILNRKLNA"
     gene            complement(21618..23081)
                     /gene="gatA"
                     /locus_tag="PLE88_03235"
     CDS             complement(21618..23081)
                     /gene="gatA"
                     /locus_tag="PLE88_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOM75513.1"
                     /translation="MDTLTQLRDRIAARHITSVEAVKAVFERIEMYEPVIGAYLSLYQ
                     DRALEQAEAVDRKIAAGQAAGVLAGVPIAIKDNMCTAFGTTTCGSKILENFHSPYDAH
                     VVEKLAQADAVIIGKTNMDEFAMGSSTENSGLKRTVNPWDISRVPGGSSGGSAAAVAA
                     GLCGAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAYGSSLDQIGPLTQDTAD
                     AALLLGVIAGWDPRDSTSVSHIPVCDYSAQLEKPVEGLKIAVVPSLLEGADSSVQKAV
                     QEALAVYQSLGARLVEIAMPHFDYAISAYYVIATAEASSNLARYDGVHYGYRSPNAGD
                     YIEVYSRSRDEGFGPEVKRRIMLGTYALSSGYYDAYYLKALKVRNLIRSDFTKAFEQA
                     DCLMMPVSPTTAFKIGEKTDDPLQMYLADVYTIAVNLAGVPGISIPCGFDRQNLPVGL
                     QIISAPFTEDKLLRIARMHEKETGWHRCRPPLKSLRQ"
     gene            complement(23094..23831)
                     /locus_tag="PLE88_03240"
     CDS             complement(23094..23831)
                     /locus_tag="PLE88_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOM75514.1"
                     /translation="MNIKTALLVAAGMSIMSGCTGKSRQAQTITQPGQHPQTLHRQTA
                     VQLDYLLYLPADYGKEQKNWPLLVFLHGAGERGSDVSRVKVHGPAKLVEQGKDLPFII
                     VSPQCPAGKWWPYMAESVIALIDEITERYPVDTRRIYLTGLSMGGYGTWAIGCMYPER
                     FAAIAPICGGGLPYLAANLKDVPVWAFHGAKDSVVPVQQSQEMVDAVNRAGGQAKLTV
                     YPEADHDSWTQTYSNDQLYQWLLSKSR"
     gene            complement(23853..24161)
                     /gene="gatC"
                     /locus_tag="PLE88_03245"
     CDS             complement(23853..24161)
                     /gene="gatC"
                     /locus_tag="PLE88_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOM75515.1"
                     /translation="MAVKIDQNQVRRVALLSRLDLTDDEVRQFSVQLSAIVAYIEKLS
                     QLNTDGVEPLAHCLPVHNVFRDDQPRPSLPNEAALANAPERYGEYFKVPRILDDSSGA
                     "
     gene            complement(24282..24548)
                     /gene="rpmB"
                     /locus_tag="PLE88_03250"
     CDS             complement(24282..24548)
                     /gene="rpmB"
                     /locus_tag="PLE88_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOM75516.1"
                     /translation="MARECYFLGKKTRTGRSIARRGKAKYLGGVGIKTTGVTRRKFKA
                     NVQKVRAVVDGKPCRLRVSAKAIRMGLVVKPPKRRWKPEQQAAS"
     gene            24738..25664
                     /locus_tag="PLE88_03255"
     CDS             24738..25664
                     /locus_tag="PLE88_03255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM75517.1"
                     /translation="MESKAEDLNKTKRVLITGCSSGFGLLTAVEAAKAGFEVIATMRN
                     LDKSHLLREALQSAGTAAQIEQLDVTSPSSIKNLTDKVGYVDILVNNAGILIMGSFLD
                     QTDAEMRKIFETNYFGIVNLTKAVVPAMMARKSGLIINIASLAGLVGHPFNAAYAASK
                     HALIGFTKSIRVELAPFGIKTVSIEPGYHRTEIIGANANQSENFYNRQSPMFEWNRGF
                     LRAMFDEILPRAGHPQAVADLIVRIMTSEKIKTHYVIGRDARFAATMQWLGLGPWLER
                     KICRKIKRTTRLENKRQAEKKARRTQKASEQS"
     gene            25667..26968
                     /locus_tag="PLE88_03260"
     CDS             25667..26968
                     /locus_tag="PLE88_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOM75518.1"
                     /translation="MAKLKLLLVNPPIYDFTAYDFWIKPYGLLQAGGLLRSIGQLWLF
                     DYLDRLHPAFDKAVSAKTDACGLGPYPEVKIPKPKPLDDIPRYYRRFGMPRGVFQDYL
                     FRNGPFDAVLIQTMMTYWYPGVREVIDDVRTISPGAKIILGGFYAVCCPEHARGLGAD
                     AVVSSDDLTALWQTLGQTPPAETCLPAWELYPRLHTAVLKLTSGCPFHCSYCFVPVSR
                     TPFKIRPLEDCLAQLRQLVSLGVRNIAFYDDALLYQPEKILLPFLRYIVERKIGVHFH
                     TPNALHARFITAEVAEWMVRAGFRTFYLGFESRCELFHRRTGSCKVAGNELEQAVQHL
                     LKAGAKGQSICAYEMLGHPQTDIQQLEDSMRFVHSLGIRIMLSDFSPIPGTPDGELCR
                     TYTNLDEPLNHNKTAFPIRYLGIKKVNYYKDLCRRLNSYLH"
     gene            27140..27949
                     /locus_tag="PLE88_03265"
     CDS             27140..27949
                     /locus_tag="PLE88_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75519.1"
                     /translation="MKKRSAFTLIELLVVIAIIALLLSVIIPALRKAKTQSKMVVCRS
                     NMRGVQTGLLLYLEANDQKMFPYSGWLWIKKLEAHVGDLDEIRYCPEIVAGVDAALQA
                     AQTANANRWGTSRQPWVWRQTDPVTGQMLYEAGGLGFNGWLYGNVNTWVPAGMENYPY
                     KTMSEVTSPSKTPVFADENWVDSWPRNTNQLHPNLLSDPQTYYNTGDTTGGTSDYAMG
                     RFLVNRHFNLRINISFIDGHVEMIDMKDLWTLAWHRGAKPNFSITLPKAKN"
     gene            28161..29030
                     /locus_tag="PLE88_03270"
     CDS             28161..29030
                     /locus_tag="PLE88_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75520.1"
                     /translation="MKKHSAFTLIELLVVIAIIALLLAIILPGLRAAKELASGVVCQN
                     NLKQLNLAWTSYYNDNNSLLVGGSNYYSGTRATPYRWVEPPLLKDTDNPEFVGIAPMA
                     QYSLQTRKNGIRAGKLFPYTQDEELYHCPGDRNITKLEPEAIYRSYAITGLMNGEDFI
                     DRYAGLYSPIRTQRTAIGKTLNVVIKFSEVRAPGNKIVFMEEDVASKATKQYYNAGGF
                     VLLGGGAFAWWDWPAYYHNDSSTIGFADGHAERHRWRDSDTLNLIKEGKADPDPVNNQ
                     DLQWLVQGYMPKP"
     gene            29076..29942
                     /locus_tag="PLE88_03275"
     CDS             29076..29942
                     /locus_tag="PLE88_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75521.1"
                     /translation="MKRNRAFTLIELLVVIAIIALLLSVVIPAMKNAKRLAAAAVCLS
                     NEGQMVKAWLMYAEQYDSKFVDGDTSDDLVRPGYTNYGSGYVWNWVGRPMGPAKQDIN
                     DTLEDKFRGFEAGGLWPFIQAPKAYHCPADLRHLRRAVYPYNSTTGNPPGLWIGGYRT
                     YSIGKVLSVRPASGSGEDRVTVSKVSEIINTSAKIVFLEETDGYGWNHRTWNMNLLSP
                     QWVDPFAILHNDSSTFAYADGHADRHKWVDQQTRDMAKAQQKGWSAVDPKTGSTQDYD
                     WFKRSYMPKQFK"
     gene            30001..30411
                     /locus_tag="PLE88_03280"
     CDS             30001..30411
                     /locus_tag="PLE88_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOM75522.1"
                     /translation="MWEHFHHQADIGIRGIGQTLDEAFAEGATALMAVICSPQQVRPE
                     KEVDICCHCDEKDLLFADWINALIYEMDVRKMLFGRFEVHIEDSCLKAKAWGEPTDPN
                     RHETAVEVKAATYMELKVYQNPAGLWIAQCVVDV"
     gene            30427..31857
                     /locus_tag="PLE88_03285"
     CDS             30427..31857
                     /locus_tag="PLE88_03285"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOM75523.1"
                     /translation="MDRKLVKRRGEYIWLLERSGPMHVDGIIFASEELISQMDDMVAQ
                     QVRNVASLPGIVEGSFAMPDAHWGYGFCIGGVAAFDPQEGGIISAGGVGFDIACGVRT
                     LHTGLKEDQLQGLCPTLAESLFRHIPAGVGSTGSLRLTKSRLDEVLAGGARWAVENGF
                     GDKDDLRFIEAGGCIHGADPACVSDEAKKRQLEEMGTLGSGNHYLEIQKVVEIYDKPT
                     AEAFGIQEGDIKVSIHCGSRGLGHQIGTDYLKKMAQAAKQHHINLPERELACAPLSSS
                     VGRQYLGAMRSGINCALANRQILTHLVREVFEQVCPKADIRLLYDVSHNTCQEEEHPV
                     DGTIKKLFVHRKGATRAWGPGRSELPSEYRPFGQPVLIGGTMGTSSYILAGTAEGRRL
                     AFESACHGAGRSMSRNAATKQYNAKDIIGRLAHQGVLIRSRSMRGIAEEAPQAYKDVS
                     QVVEATHRAGLARKVAKLIPLVCVKG"
     gene            32065..32856
                     /locus_tag="PLE88_03290"
     CDS             32065..32856
                     /locus_tag="PLE88_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75524.1"
                     /translation="MKRLRNDMYACGQSGLPQAIYLNGQKWTLETVFKHDFFACTGRY
                     QCHLSTEKVVLKISRLQSFFGIPCAWLGRMLRNHELTILRHLADIEEVPHIIRSFGHN
                     GFLYYFIEGQSLDEKPAVPDDFFSRLRALLAAIHKRDVCYIDLNKRGNILLGKDGRPY
                     LIDFQISLHLPGRWCRFLRTAIQREDMYHLLKHHRKFRPDLLTPEETQRSRQPSGLIR
                     LHRLAAHPYRQIRRALFRFLYKSRILTPDDSHHRTPENDPNRFLQ"
     gene            32989..33879
                     /locus_tag="PLE88_03295"
     CDS             32989..33879
                     /locus_tag="PLE88_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOM75525.1"
                     /translation="MIFDIKKYAIHDGPGIRTTVFFKGCPLRCSWCHNPESWSDKLEL
                     MFRAARCVRCGRCAQACPHGAIRMDGLPVTDGSVCRQCGACIPVCGQQAREIAGRKVG
                     VGQIMDEILKDVIFYDDSGGGVTFSGGEPLMQPEFLEALLEECRNREIHTAVDTSCFA
                     PQSVLKKIIPLTDLFLCDIKHLDSEKHKRFTGVDNKQILTNIRFLVENGCTIIVRIPV
                     IPGFNDTAEAVDAIARFVKTMNTIHQIDLLPYNSGGVSKAQRLGGRIQILHGRRPDKE
                     LMAELENCVRQYGLKTTIGG"
     gene            33882..36242
                     /locus_tag="PLE88_03300"
     CDS             33882..36242
                     /locus_tag="PLE88_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOM75526.1"
                     /translation="MNERVRKLRDQSVNTKPYISAERAVLITDFYQSGIALQVSAPVL
                     RGMALKHIMEHKSLCINDGELIVGERGPAPKATPTYPELCCHTLEDFEAMSRRQRTSF
                     DVSDDVKQVYAEKIIPFWKGKTIREKVFASMPEAWHKAFEAGVFTEFMEQRAPGHAIL
                     DDKIYKRGLLDLQDDIRQSLRQLDYFNDPRAYEKEQELKGMSLAIDAVLTLARRYSEE
                     CAKLAQQQTDPQRRSELEKMAAICRHVPAYAPRTFHEALQAYWFVHLGVITELNVWDS
                     YNPGRLDQHLWPFYQKEIAEGTLTREQAKELLECFWVKFNNQPAPPKVGITEEQSGTY
                     TDFALINIGGVDPQTGADAVNDVSYLMLDVVEEMRLVQPSACIQISAKNPDRFLKRAC
                     KVIRCGFGQPSVFNTDVIVREMLHHGKSMVDARSGGPSGCVETSAFGKESCTLTGYCN
                     WPKILELALHNGIDPRTGIQVGPATGKAETFDTFEKLLDAYRQQLGYFIDLKIRGNCI
                     IERIYANFMPAMFLSVLTDDCIKKGLNYHNGGPRYNSTYIQGVGLGTLTDALAAVKYN
                     VYDKKTVTMETLLKALKADFQGYTELHNQLEASPRYGCDNDYADELALRVFNIYYDLI
                     NGRPNTKGGHYRINLLPTTVHIYFGQITGALPHGRKAGLPLSDGISPTQGTRCPGPTS
                     IIKSASKIDHSRTGGTLLNMKFNPQVLEGPGLEKLGHLIRSYFKLDGHHIQFNVIRKE
                     TLLSAQKNPAEYKDLIVRVAGYSDYFIDLGTDLQNEIIARTEMTEI"
     gene            complement(36291..37349)
                     /gene="ruvB"
                     /locus_tag="PLE88_03305"
     CDS             complement(36291..37349)
                     /gene="ruvB"
                     /locus_tag="PLE88_03305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOM75527.1"
                     /translation="MAIDRVLSADSGGDKEEQFNIALRPQRLAECIGQTPVRDKLSIA
                     IQAARRRGEPLEHILFYGPPGLGKTTLAHVIANEMGANIRVTSGPAITKQGDLMGLLS
                     NINTGDVLFIDEIHRLSAPVEEFIYPAMEDFKVDFTVDSGMHAKTINFPLKRFTLIGA
                     TTRAGLLSAPLRGRFGMLYHLEFYNVGELTEILKRSAALLELKAENGTLELIAGRSRG
                     TPRIANRLLRRVRDYAQVKGSGKLTAEIVESALRMEQIDPLGLDELDRSFLRALVKVY
                     DGGPAGIEALAATLGEERDTLEDVVEPYLLQIGFVRRTKRGREITSAACRHLGLADVR
                     KQKPADDDALLFDENTDE"
     gene            complement(37379..38005)
                     /locus_tag="PLE88_03310"
     CDS             complement(37379..38005)
                     /locus_tag="PLE88_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOM75528.1"
                     /translation="MIAQIEGKLVSLTEDTALIQAGSFCYEVMLPGYAVMQLGNRLGQ
                     PVTLCTFEYYEGTLGGGNLIPRMVGFLSRFERDFFDIYTGVKGMGIKKALKSLALPID
                     RIATAIESGDEKLLATLPGIGKKMAQTVSSELKGKLTAYALQTAAAQSRSAFSAYQME
                     ALEILIAWGEKRAEAMELIELACRKHPEIKTAEELVPLVYRIKQGVEV"
     gene            complement(38041..38520)
                     /gene="ruvC"
                     /locus_tag="PLE88_03315"
     CDS             complement(38041..38520)
                     /gene="ruvC"
                     /locus_tag="PLE88_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOM75529.1"
                     /translation="MKILGIDPGLQVCGYAVVERKRLDTRLVEAGVFRTDGRAELEKR
                     LNQIAADIGQILQAHRPQIVAVEQLYAHYKHPQTAILMGHARGVILQKAAESGASVKN
                     FSATRIKKSLTGNGRAHKGQMQRAIQTVLALPDVPKPPDVADAIAIALCCASEHQAV"
     gene            complement(38517..40004)
                     /gene="cysS"
                     /locus_tag="PLE88_03320"
     CDS             complement(38517..40004)
                     /gene="cysS"
                     /locus_tag="PLE88_03320"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOM75530.1"
                     /translation="MTVRLYNTLTRTTEEFRPLHEGRVGMYVCGPTVYGHAHLGHGKS
                     YVSFDVLLRYLRYLGYAVTYVQNITDVGHLTDDADAGEDKISKQAQREKKHPMAIAEY
                     YTRSYFEDMDALNCLRPDISPRATGHIPEQIELVQILLDKGYAYEADGSVYFDVSKFP
                     AYGRLSGRNVDEMLAGARVDINAQKRHPADFALWKKAEPSHIMQWNSPWGRGYPGWHL
                     ECSVMSMKYLGKTIDIHGGGIENQFPHHECEIAQSEAANGVPFVRYWIHHNMVTVDGQ
                     KMGKSLGNFITLKQIFDVCPVERHERLSRKYSPLAVRQLILQSHYRSPIDFSDAALTA
                     AQSGYDRMTEAVLAVRKAIRTAPAGQVDADAAGRLAAMRQKFEAAMNDDLNTAVALSV
                     IFEMVKLAHQTLAGKPTQETLRAVDEQFRRLGGDVLGIVKEDYPSAGGGDEQLLDHLI
                     GLMIEQRQAARKKKDFAAADTIRNKLAEFGIVLEDTPAGTVWRRQ"
     gene            complement(40001..40510)
                     /gene="ispF"
                     /locus_tag="PLE88_03325"
     CDS             complement(40001..40510)
                     /gene="ispF"
                     /locus_tag="PLE88_03325"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOM75531.1"
                     /translation="MDGLDYEYRTGIGTDIHRLVPERPLMLGGVQVPFELGLLGHSDG
                     DAVLHAVIDAMLGAAGLGDIGMLFPDTDPQWKDADSSELVMRAREQVKKHQWEVVNLD
                     VVIHAQLPRLGPFKGQIKRQVAGLLETDFANVNIKAKTNEGLDAVGRGEAIAATAVIM
                     LRRRIKRRL"
     gene            complement(40514..41098)
                     /gene="hisB"
                     /locus_tag="PLE88_03330"
     CDS             complement(40514..41098)
                     /gene="hisB"
                     /locus_tag="PLE88_03330"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOM75532.1"
                     /translation="MAKRKAEIRRKTNETDIRAELHLDGQGCYEIDTGIGFFDHMLTH
                     LSKHSRMDLTVKAKGDLSVDAHHTIEDVGICLGQAFLEALGDKKGIARYGHSVVPMED
                     AKAVVAVDLSGRPYLVFHAQFPSGKIGEFDTECVEEFLRSFSTAGKFNLHAEVPYGSN
                     SHHIAEAIFKALGQALGAAAAITGSQLPSTKGVL"
     gene            complement(41109..42149)
                     /gene="hisC"
                     /locus_tag="PLE88_03335"
     CDS             complement(41109..42149)
                     /gene="hisC"
                     /locus_tag="PLE88_03335"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOM75533.1"
                     /translation="MGYFRPTIEQMAGYTPGFQPKSTAVVKLNTNENPYPASPRVIQA
                     VQTLDAEKLRRYPDPLGQAFRLAAAEVWAVEPDNIICTNGGDDLLNICVRAFCDAQRP
                     VAYAQPTYSLYPVLAQLQGCPAIEVRRDAGGSLAELAQINAALTIVCNPNAPTCDFLP
                     AEALERLAAHLNGVLLIDEAYADFAEDNALRLVKKFDNVIILRSMSKGYSLAGMRFGC
                     GIAHKNLVAGLMKVRDSYPVNAAAIAAAAAAVSDQAYFADNIRKIKLERTRLIGRLRA
                     LGFDVPDSQTNFVLARCVQKEAKAIYNALTEQNIFVRYFELPGLEDKLRITVGTPEQN
                     DTMLAALKRLLD"
     gene            complement(42231..43076)
                     /gene="accD"
                     /locus_tag="PLE88_03340"
     CDS             complement(42231..43076)
                     /gene="accD"
                     /locus_tag="PLE88_03340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOM75534.1"
                     /translation="MVKKKKASWNGFSLKPRREMPEGLWLSCPSCRNMLFRKTVDENL
                     SVCPECQYHFRIDGVTRLRQLADEGSFEEIGSDLASSDPLQFTWGDKSYKDRIQIDPA
                     QMSAHEAMLAGKAFIRGRGVMLAVMNFSFLGGSMGMVVGEKFCMAVDRAIEESLPLVV
                     VCCSGGARMHEGLVALQQMAKTSAALARYDQAGGLYISVLTDPTTGGVAASFALLGDI
                     IIAEPKALIGFAGPRVIQQTIKVELPEGFQTSEFLLEHGFIDMIVHRKNLRSEIGRLI
                     DYCGK"
     gene            complement(43086..43778)
                     /gene="rpe"
                     /locus_tag="PLE88_03345"
     CDS             complement(43086..43778)
                     /gene="rpe"
                     /locus_tag="PLE88_03345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOM75535.1"
                     /translation="MRRLPAAGTHQIVPSILSADFANLSSEIAQVMHEGITMVHLDVM
                     DGHFVPNITIGPPVVKWIRQCTDAVLDTHLMITDPQTYAPQFVKAGADHITFHIETVD
                     NPADFVNMLRDMGVTVGITLNPPTPVETIEKVAPLCDMVLVMTVNPGFGGQSFIEKAA
                     QKCRRVRQIAGPNVRIEVDGGIDAATIGTARDYGADTFVAGNAIFGRTDRAAAIAALR
                     KALADSRFPLQQ"
     gene            complement(43865..45103)
                     /locus_tag="PLE88_03350"
     CDS             complement(43865..45103)
                     /locus_tag="PLE88_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOM75536.1"
                     /translation="MAKKTVADINVKGKKVLMRVDFNVPLDKNQNITNDNRIVQALPT
                     IQHVLAGGGALILMSHLGRPEGAPDPKYSMAPVAKRLGELLGRPVQFVGDCVGPEVKA
                     KAAALKPGDVLVLENVRFHPEEVIKDKAAKEDPALRAAKDAFAKQLADLADVYVDDAF
                     GTAHRDNASMYTVPCMMKDKPRVSGFLIEKELKFLGETVEKPERPFVAILGGAKVSDK
                     LKVIENLLPKVNCLLIGGAMAYTFFKAKGQTVGKSLCENDFVENAKGLIQKAKEIGCE
                     MLLPVDTVIAAEFKAHAEHKVVEGSIPDGWEGLDIGPKTATLFAEKIKTAKTIVWNGP
                     MGVFEMPPFDAGTKAVAKAVADATAHGARSVIGGGDSASAVMQMGLADKVSHISTGGG
                     ASLEFLEGKKFKCLDILEDK"
BASE COUNT        10141 a        12583 c        12008 g        10404 t
ORIGIN      
        1 ccgccccgct taccccgcac agcccccagt agccccagcg gggaatcgtc agccagaatc
       61 ctttgaccgg agcagccgcc ggttcaggac ctgtaatatg ctcttccgcc cggtctatca
      121 gttcctcatc aaccagcggc ttgaccgtgc ttttgaatag ggtttggttc agtttcagca
      181 aagccgcctg ctcttgataa gtctggctct gccgggcgta gtcctgacgg gcacggtccg
      241 ataaatgcac cacggcaaat gcccccagac acgctcccag cagcgctccg aacaaggata
      301 gagattgaat gaactcttta tcttcgtgtc gtatcggcat aatccacctc aataagctat
      361 tgtctctgca taaactataa caaatggccg gcagaacagc aggccggaca gaaaataatt
      421 ttattgaaat aatatctcac ggcttgtaat attaccaaaa accagtattt gcaaggacga
      481 tgcctatgga taaaaaaagc gtgtctttct tcattgtggg gattctggcc ggcattctta
      541 tctccgtcgg gggctttacg tggtatgtgc gcagcgctgc tgtcgggggc gggcagcgaa
      601 atgaggtgct ggtgctcaaa ttaggacacg gattggaccc gtcgcatccg gtccacaaag
      661 gaatggtcta tatggccgag cgtctgaaag agaaatccgg cgggacgatt gagctgcaga
      721 ttttccccgg cggcattctc ggctcggaaa cggagtgcat tgaactgctc aaaaacggcg
      781 ccttggcggt taccaaagta tccaccgccc cgctggaagg atttctgccg gagatggggg
      841 ttttcagtct gccgtatgtg ttccgcaacg aggagcatta ctggaaggtg ctgaccagtc
      901 cgctgggccg gaaactgctg cagatgggcg agccggccgg tctgcacgga ttgtgctact
      961 atgactccgg cagccgcaat ttctacacca ccaccaaacc tattctgacg cccgatgacc
     1021 tcaaggggct caaaatccgc gtgcagaaca gcaagacctc gatggatatg attgaagtgc
     1081 tcggcgcggc cgcaacgccg atttcgtggg gcgagctgta caccgccctc cagcagaaaa
     1141 tggtggacgg ggccgaaaac aatcccccca gttttgtcac cagccgccat tttgaagtct
     1201 gcaagtattt ctccttcaat gagcatacgc ggattccgga catcctgctg atgagcaaga
     1261 aaatctggga cacgctgacg ccggcccagc agcagtggat tcaggaggcc gccgacgaat
     1321 catcgcttta ccagcgtcag ctctggaaga caatgacagc cgaatgcatc gagacgatga
     1381 aaaaggaagg cgtgcagcta ttttatccgg ataccaagcc gtttgaagag aaggtgcaac
     1441 cgctgctgcg ccagtatgag gggactccaa tcggcgatat tgtccggcaa atcagggaaa
     1501 tacaataatg ggaacatggc ttcgacggat taaaaaaggt ctgcaaaccc tgctggaatg
     1561 gggggtcatt gtcatcatgg cgattctggt gctggatgtg ctgtgggggg ttttttcccg
     1621 ctatgtcctg aaagcccaga gccgctggac ggaagagctg gccatctttc tgctggtttg
     1681 ggtgtcgctg ctgggggcca gcgtggcgta tgcatccaag tcgcatctgg gggtggatta
     1741 ttttgttggc aagctggacc gccaagccca gcggctggcg gagctgctgg ttcatctggc
     1801 tgtggcggtg tttgccgcct cggcctttgt cgcaggcggc tgggcgctgg tatccaagac
     1861 attggccgcc ggccagattt cgccggcact gggcgtcaag atgggctatg tttatctggc
     1921 ggtgccaatc agcggggtat tctttctgat ttttgcggct gaaaatatcg ctgaactgtt
     1981 cgccggcaag ccgactgctt ccgcgatgca atccaaggag aactgatccg tggaaacaca
     2041 ggtattgatt ctggtcatca gttttacagt ccttctgctg atgaatgttc cgattgctgt
     2101 ctgcatcggg ctgtccaccc tgctgacctt ttattccatc ggcgatattc ccaccgccta
     2161 taccgtcgcc cagcggattt cgatgggcat tgccagcttt ccgctgctgg cgattccgtt
     2221 ttttattctg tcgggcatcc tgatgggcga agggggcatg gcgcggcggc tgattaattt
     2281 tgccggctcg gtggttggat ggctgaccgg cgggctgggc tatgtcaata ccctgacatg
     2341 catgatgttc ggctcgattt ccggctcggc ggcagcggcg gtttcgtcgg tgggcggctt
     2401 tatgataccc gagatgaaca agcagggcta cgacaaggcg tttaatgtgg ccgttaccac
     2461 caccgccgcc acaaccggtc tgctgattcc tcccagcaat gtgatgattg tctatgcggt
     2521 ggtggccggc aatgtctcgg tggcggccat gtttatggcc ggcgtgctgc cggggattct
     2581 tgtctggctt gccattatgc tggtctcggg catcatcagc catatccgcg gctacggggc
     2641 ttcggatacg gtatccgtcc ggcggatgct gaagaccttt ctggaggccc tgccctcgct
     2701 gctgctggtg gtcatcgtgc tgggcggcat cctgggcggc attttctcgg ccaccgaagc
     2761 atcggccatt gccgtggcct attcctttct actggccgtg ctgttttacc gcgaagtcaa
     2821 gctgaaagac ctgccgcgca tcctgctgga atgcggccga accaccgctg tggtgatgct
     2881 gctggtcggc tgcagtcagg caatgagctg gattttggcc tatgaaaaca ttccgcagag
     2941 cgtcagtgcc tcgctgctga aaatatccag caatccggtg gtgattctga tgattatcaa
     3001 tgtgctgctg ctggtggtgg gcacttttat ggatatgacg ccggcggtgc tgatttttac
     3061 gccgatattt ctgccggtgg ttgtcgggct ggggatgaat ccggttcact tcggcattct
     3121 tctgattgcc aacctgtgca tcgggctgtg cacgccgccg gtgggcacgt gtctgtttat
     3181 cggctgcggc gtcggcaaaa tcagcattgc caaggtgctt ccgtcgatga ttccgttctt
     3241 tattgcaatg attggggcct tgatggtcat cacctactgg ccatggctgt cgctgtggct
     3301 gccgggcatt atggatctgc tgtaaaagcc ggcggcggaa gcggccgttt ggaaaaagca
     3361 tgacaacagc aagcaaaaca agcattgtgg ctacgctggg gccggcctct tcggatgcgg
     3421 cggttttgga atcgctggtc cgccagggcg tgtcggtgtt gaggctgaat ttttcgcacg
     3481 gcacctttga aacgcacgcc caaacgctgg cccacatcag ccgcatccgc aagagcctgc
     3541 cgtatgaaat cgccgtgatg ggcgatttgt gcggcccgaa aatccgcctc gggcaggtcg
     3601 agccgcaaac ggttcttgcc cagaatgccg aagttatgct gtcggccggc acagccgtcg
     3661 gcacggcggc acagctgttt accgattttg aaccgctggt ccgcgatgtc cagccgggac
     3721 agcggattct gatcgacgac ggccggctcc tgctgcaagc gctggaaaag acggccgccg
     3781 gcatccgctg ccgggttgcc gtcggcgggc cggtttccag ccgcaaaggc atcaacctgc
     3841 ccgatacccg tctggcgatt caggccatca ccgaaaaaga ctggcagtac gccgactggg
     3901 ctgctgaaaa ccagctggat tatctggcct taagttttgt ccagcgggct gacgagattg
     3961 tccggcttaa agcgtatctg gcttccaaag ggtccggcat tcgggttatt tccaagattg
     4021 aaaagccgct ggcggtggaa aacatcgaag cgattatccg ggcctcggat gcggtgctga
     4081 tcgcccgcgg cgatatgggg gtcgagatgg atttggccca ggtgccgctg gtgcagaaaa
     4141 agataaccgc cctgtgccgg cggcttgcca agccggtgat cgtggccacg caggtactcc
     4201 agtcgatgat agaaaatgcc tcgccgaccc gcgccgaagc aaccgacatc gccaacgccg
     4261 tgatggacgg ggccgatgcc gtgatgctgt cgggggaaac ggctgtcggc agatacccgc
     4321 tggcggcagc ggcggccttg tcgcatatct gccgtacaac cgagcagttt ctcgaagccg
     4381 ccagcagcgt acgggcgccg atggaaaccc cgcccgagca gattgaaaag gcagccattg
     4441 cccgcgctgt tgcccagatg ctcgatgagg tcaaatgcgc ctgcattgtg gtctggacaa
     4501 agaccggcca gatggcccac ctgctgagca aggcccatcc ggatgtgccg attgtgtcgc
     4561 tgtcggccaa tcggcttctg gcccgtcagc tggcgctttg ccggggcgtg gtcagcattt
     4621 atcagccggc cgtctccgct tatgaggaat ggatcgggct tgtccaccgg ctcctgcttg
     4681 ccggcagctg ggcagccgcc ggcgataaag tccttctgat tcctccgctg gagctgctga
     4741 cagcccaaac cagcggcgca ctgattctgc atactcttgc aaagtaagcc cgctgttttt
     4801 tttctgccct gctctttttc caaagccgag ctgccggaca ggcagtctgc cggcgcagaa
     4861 tttctgccgc cgaatccatt gaggattttc tgcttgctgt gtataatgcg gctggtcaga
     4921 gataaaagga ttgacgacga tgaaaaaaat atattttctg cttttcttgg ccgccggatt
     4981 gacctgtgcg gctgccattg accgcttttc gctggtcaat cggcacaata tcaccctcac
     5041 ccggttcgat gccttttcgc cgctgtcggt cggcaacggg cattttacct tcaccgccga
     5101 catcaccggc ctgcagacat ttcccgaagc ctacgccaag ggcattcccc tgacgacgat
     5161 ggctgaatgg gggtggcatt cgtttgccaa caccgaaggg tacaagctcg aagatacctt
     5221 tgtgcaggtc gatacctacg gccggccggt gccttacaac agcaaccaga aatgccctgc
     5281 cgccgattat ctgcgtgcca acccgcatca gattacgctg gggctggcgg ggcttgtctt
     5341 gacgcgaagc gacggcagcc ctgcccgttt ggaggatatc ggtgccatcg agcagcagct
     5401 gaatctgtgg cagggactgc tgaccagccg gtttgagctg gaaggccagc cggttgccgt
     5461 tgagacctgc gttcatcccc aactggatat ggcggcggtg cggattcgct cgccgctgat
     5521 agcgcagaag cggctggcag ttgtgctgcg gtttccgtat gccgccggca catggggcag
     5581 cgacccggcc gattggtctt cgcccgacaa gcatcagacg ctggttgccg tccgttcgga
     5641 agatcgggcg gttttcgagc ggattatgga tgacaagcac tattactgct atacggcttt
     5701 ttccaacaat acccagctgc agcagaccgg cccccataca tatcttttcg tgccgccggc
     5761 cggcgatgca ttcgattttt cggttctctt tgacccggct gccggcatac cgcagatgcc
     5821 cgatgccgcg gcgacgcaag cggtgtgtgc agaacactgg aaggcgttct ggacatcggg
     5881 cggtgcaatc gatctgtccg aaagcaaaga tccgcgctgg aaggagctgg agcggcggat
     5941 tgtgctgtcg cagtacctga cggcgattca atcggtgcag aaatatcccc cgcaggaaac
     6001 cggcctgacc tgcagcagct ggttcggcaa gtttcacctc gaaatgcact ggtggcacag
     6061 cgtgcatttt gccctgtggg ggcgtctgga gatgttcgaa tcggcactgg attggtatcg
     6121 ggagattctg ccggcggccc ggcagattgc ccagcggcag ggatacaagg gcgtccgctg
     6181 gccgaagatg acagacccat cggggcagga ttcgccttcg ggcatcggac cgtttctgat
     6241 ttggcagcag ccgcacccaa tctactatgc cgagctggtt tatcgccaaa agccgacagc
     6301 ccaaacgctg gagcgattca aagacattgt gtttgagacc gccgagttta tggccgactt
     6361 tgcccattgg gaccccgtca gcgaaagctt tgtgctcggc ccgccggtta tccctgcaca
     6421 ggaaaattac aagtacgaca agacccgcaa tccgacctat gagctgtgct actggtactg
     6481 gggactgcag acagcccaga aatggcgcag ccggctggga ctggatccgc agccgcaatg
     6541 gcagcatgtg cttaacaaca tcgccctgct gccgatgcgg gacggcattt actgcgctgt
     6601 ggaaaccgaa cccttcacca acctgaccga ccatccgtcg atgctggcgg cactgggcgt
     6661 gctgccgccg acgccggcag tggatgcggc agtaatgaaa aagaccgctc aatgggtcta
     6721 tgacaactgg caatgggcgg acacatgggg ctgggattat ccgatgctgg cgatgaccgc
     6781 tgcccgcgtg ggcctgccgg aactggctgt tgaggccttc tttatcgatt ccaaaaagaa
     6841 cctttattgg cccaacggcc acaactacca gcgggataac ctccccctgt acctgcccgg
     6901 caatgggggt cttcttgccg cagttgcgat gatggccgcc ggctgggacg gctgccccga
     6961 ccgccccgct cccggctttc ccgacaacgg ccaatggacc gtcaaatggg agaacctcca
     7021 aaaaatgccc taaaggaacg caggcattct gcaactgctg ggagggaata cccgtgcgcg
     7081 tctcaaccaa gcgcgacgat ttatccccaa ccatctgaca gccgcttgtc aataacagcc
     7141 ccgccaacgc tgcctgctca tcgtttttat caatctatct acatagttta ttcatatgcc
     7201 gccttcagcg cggccttcaa atcaatcgtc ccttcataga gcgcccggcc aataatggct
     7261 cctgccacgc cggcacgctt aagcgcctta acatcctcta ccgtcgtaac acccccggcc
     7321 gcaacgacag gcagttcgac cgcctccacc agctgccgcg tccgctcgat attgggtccg
     7381 gctagcatgc cgtccttgct gatgtcggtg taaataatcg ccgccagcgg cagatcggcg
     7441 gcttttttcg caaattccag cacggtttgg gcggcctctt ccagccagcc ctcggcagcc
     7501 agcgtgctgc cgcgggcatc cagccccaag accagtttgt gcgggtattt ttcggccatg
     7561 cggcaaaacc agtcaaactg cttgacagcg ctgcttccca aaatcagccg ggttacgccg
     7621 gcctcgagca tctggacaat cgcctcttcg gtgcgtatcc cgccgcccag ttcgattttc
     7681 atcggcacct gtgcggcaat gtgtgccacc gtttcagcat tgaccggccg gccggccttg
     7741 gcgccatcca aatcgaccag atgcagccag acagcgccgg cctgataaaa ctccgccgcc
     7801 tgcgcagccg gatcatcccg ataggtaatc tgcctgtcgt agcagccctg aatcagccgg
     7861 acgcaccggc cgcctatgat atcgattgcc ggaaggatat ccacggtgct acaactcgta
     7921 caactgctgg ccggaaacca ccttaaagcc gtaagcggcg atggcctctt ccagattgac
     7981 ctgttcggga ttttcaaact ccataaagca gatgccctgc cggtctttga gtgacacaaa
     8041 gccgtaggcg tccttgatgt tgacgccccg ctccagcagg gctgttgtca gcttgtccaa
     8101 gttgccgggc tggtcctgtt cggccaccac cgccagcacc tctttgagca cacaggcaaa
     8161 accgcggtcg gccaaaatca gctgggcctt ttcgggctta tcgacaatca ttttcatcag
     8221 gccgaattcg ccgcggtcct gaatggtaaa cgcccagata ttcagactgt tttccagcag
     8281 cacatcggaa atggatttga tgcgtccggg acggttttcc agaaaaatcg ttaattgctt
     8341 ggccatagtt tccctctgta gcattagagt ttttcccgct ggtcaaccac gcgtttggcc
     8401 ttgccttcaa aggccggcag cgcgccgggt tcatgcagtt cgatatgcgg attgatggtg
     8461 atagacgccc gcaggcggtc gcggatttta ttcttcaggg cttccagagc attcaaatcg
     8521 ccggtgaaca tcttggaata aatctccacc ttgaccgtca gcttgtccag cgcgccggcg
     8581 cgggtcagat gaatcatata attggtgccg acttccggga tggacataat cacttcctca
     8641 atctgcgagg ggaagacatt gacgccgttg ataatgagca tatcgtcggt tcgtccctga
     8701 atgcgcgaca ggcgacggtg ggtgcgtccg cagggacagg gttcgggatg cacaaaggcc
     8761 aaatcgcgcg tgcggtagcg catcagaggc attgccatcc gcaccagatt ggtcagcacc
     8821 acctcgccca gctggccgtc gggcaggacc tgtccggtgg ccggatcaat gatttccaga
     8881 atgtaggcat cttcccacag gtgcatatcc tgcttataga cgcactcaaa ggcgacgccg
     8941 gggccgttca tctcgctgag cccataggaa ttgtaggcgt cgatgccgaa cagctcctgc
     9001 agcttctggc gggtgttttc ggaatagggt tcggcgccga gaaacgcttt tttcagatgc
     9061 agatcgccgg gcttgacgcc gaactcgtcc agacgcgaat ggatatgcag cagatagctg
     9121 ggggtgatgt gcagcacggt ggttttaaaa tccttcatca cctgcacctg ccgctgggtg
     9181 tttccgccgc cgatggggat gacggtcatc ccgacgcgtt cggccccgta atgcaggccc
     9241 agaccgccgg taaacaggcc gtagctcatc atattctgaa acacatcctt ccgcgtgcag
     9301 ccggtggcgg tgatgctgcg ggccagcaga tcggtccagg cattgaggtc ttcctgcgtc
     9361 aggtaaataa ccgttggaat gccggtggtg ccgctggagg aatgaatgcg aaccacctcg
     9421 tccatatcca ccgccagcag cccgacagga taattctgcc gcaggtcatc tttggtcgta
     9481 aagggcagtc tctgcatatc cgccagcgag cggaactggt cggggctggt gatgcccgcc
     9541 cgcgacaggc gcttctggta aaagggggtt ttgagcgctt gggcgatgat gctgcgcatc
     9601 cgctgaatct gaagctgctc cagagcagcg cgttccatcg tttcggcctg acgattccag
     9661 taagtcattt ccattgctga ttcctgaagc gttttttatg ctcttttttt acagcccttt
     9721 gatatcccgc tggccgacaa ccttgatatg attctggctg agaacctgat gggcatattc
     9781 aggattgtcg aaacgaaaga ccagcagggc attttcggaa aacttttcga taaatccgta
     9841 catgtattcg acattgacat tgtggtccga cagaaccttc agcacttcgg ccagcccacc
     9901 gggcttgtcg ggcacttcga cagccaccac ctcggtctgg ttggctgtaa cactgttgcg
     9961 gcggaacagc tcgacagcct cggccggctt atcgaccacc atccgcagca caccaaagct
    10021 ttcggtttcg gcaattgtca acgcccggat gttgatgccg gccttgccca gcatcgagca
    10081 gatttcgtac agccgccctt tgcgattctc cagaaatacg ctgatttgtg tgattttcat
    10141 aacacggttc ctttcttttc acaactgccg cttgtcaata acacgtttgg ccttgccctc
    10201 gctgcgctgg atggtcttgg gttcgaccag tttgaccgtt acgccgatgg acaggacggc
    10261 ctcgatttcc ttcttaatct tctgctccag cgcatgcatc cgcttgattt catcggagaa
    10321 gattttctcg tccacctcga ccataatctc gacctcgtcg agcttgtggg cctgccggtc
    10381 aataacaatc tggtaatgcg gatgggtgcc ttcgatgccc agcaggactt cttcaatctg
    10441 ggacggaaag acattgatgc cgcgcacgac aatcatatcg tcggtgcgtc ccttaatctt
    10501 gctggtgcgc gggctggtgc gtccgcagcg gcagggctgg gtggtcatgg agacgatatc
    10561 ccgcgtgcgg tagcgaatca gcggaatgcc ctgcttggtc agcgtggtga tgaccagttc
    10621 gccgtcctgc ccttccccaa caggccggcc ggactcgggg tcgatgattt ccgggtagaa
    10681 tacatccgaa tagatatgca ggccgtcttt gtagtggcac tcctgcgaga cgccggggcc
    10741 gataatttcg ctgaggccgt acacatcggt tgccagcata ttccacgtct gctcaatttc
    10801 cgtgcgcatc gattcactcc acggctcggc tccgaagatg ccgatattcc agctggccgc
    10861 gcgcggatcc atgcccatct cgcgggcttc ctctgccata tacaggcagt agctgggtgt
    10921 gcagcagaga atccgcggct tgaaatcctg catgattttc agctggcgtt tggtttgtcc
    10981 ggaggaggtc gggataatgg tcagtcccat cttcagcgcc ccgtaatgga agcccaaacc
    11041 gccggtaaac aggccgtagc cgtaggcaat ctggacggtg tcatccgtcc gggcgccggc
    11101 acagcagagg gtgcgtgcca tgacttcgct ccacagttcg atatcatcgc gggtatagcc
    11161 gaccagtgtt gggttgcccg ttgtgccgct ggagacgtgg atttccacca agtcggtgcg
    11221 cggcacagaa aacaggccga aggggtaatt gtcccgcata tccagcttgg tggtaaacgg
    11281 cagtttggta atgtcgtcaa ttgaatggat gtccgacggg ctgatgccgt ggtcgtcaaa
    11341 tttccggcgg tacacaggac atttttcata gacatagcgg cacagcttga ccagacgttc
    11401 ggactgaaga tttttcaaat cctccagcgg cattgtttcg acaggttcgt tccaaatcat
    11461 acgtccactc cttgataaga caggatcttt tcttattgct gcaacatcag gcgtccggcc
    11521 atgcggaaga cagcctgaat cttattttac aagagctttg gcccgctgga aaacctgcct
    11581 gttttcgtcg cgaatcttgg gcgaaaagac cgtttccatc gccttctgcc agccggcctc
    11641 cgagataggc agaaagacgc tcagcacgcc catcagggcg gtattgacgt gcttgggatt
    11701 ttccaccatc gcctcggcct gctggagata gccggccaaa tccgtgccgc cgggcttgag
    11761 aaacatcacc agccggggat gctcatccag atgaaagcac agcagaaaat cagcctgacc
    11821 gggtcctatc agcggcgaat agattttcct gccgaagcgg acatgggact ccaccgaacc
    11881 gccccgctgg ctcatcccgt ggacttccga tttcttgaca tgccagccgt cgtacatcgc
    11941 cgcccagccc agcagctcgg aggccttgat aatcccctgc ccgccgatgc cgccgaatgt
    12001 aatattatag caatcctgtt ttttcatcgt ctgtactctg cctttaccgt tttatgagtt
    12061 tgcacgggtg tttggcaata atgaccgtca gggcatcctc gctgaggcgc cgccgaacca
    12121 attcttccag ctgcccgaca ttgccggccg gatccatcac atccacatta tccgccccgc
    12181 aggccagaca caaggccttc aggtccagcg tcttggtggg ctggccgcgg atggtcaggc
    12241 cggtaccggg gtgattctgt ccgccggtca ttgcggtcgt ggaattatcg aggataaaga
    12301 taatcccctt ggccttgttg taggcggcgt taatcagacc ggtaatgccg gcgtggatga
    12361 acgtcgaatc gccgataaca cctacaattt tcccgtcggg gtgagcacgg cgcaggcctt
    12421 cgttgaaggt gactccggct cccatacaca ggcaggtatg cagcgccgag gtcggcggca
    12481 gcgtgcccag cgtatagcag ccgatatcgc cggccacaaa cagtcccagt ttcttcagca
    12541 cggaaaaggt tgcccaatgc ggacagccgg cacacagccg gggcggacgt gccggcggca
    12601 gcttgacagc ctttttcggc cggccggcaa tcacatcctc tatcacgccg gggcctaatt
    12661 cgcccagacg gaacgatgga tgccggaata cggccttttt gatgcccagc tggcgaatgt
    12721 ggtcttcgat gaaggggtcc agctcctcca ccacgaccag ttttttaacc ttgccggcaa
    12781 acgccttgat tttggcatca cagaacggat agaggaaacc cagtttcagg aaggacgcat
    12841 tgggccgggc ttctttgaga taataataac tgacaccgcc ggcaataaaa cccaccgata
    12901 ggtcattgag ttccatcttg ttgagccggc tccgttccga cagcttctgc agcttgagct
    12961 ggcgctgttc aaccagcgca tgccgctgac gggcaaaagc cggcaccatc acatatttct
    13021 gtatatccgg cttcaggggg cgtttttctg ccggccggcg ctggcccagc agcacatctt
    13081 ccttggagtg ggacacccgc gtggtcattc ggaggatcac cggcgtatcg tacttttcgc
    13141 tgatggcgaa tgcctcggcc acaaactgct tggcttccgc gggactggcc ggctccagaa
    13201 tcggcagttt ggcataaatg cccgcccagc gggtatcctg ctcgttttgg gacgaatgca
    13261 ttccgggatc atcgcagaca accgccacaa agccggcatt gacgccggtg taagaggagg
    13321 tcatcagcgg gtccatcgcg acattcaagc ccacatgctt gcaggcaaac aggctgcgta
    13381 cgcccccgac agcggcgctg taggcgactt cgtaggcggc tttttcattg acggaccact
    13441 ggacatccag ttcgggatac aacgccagcg attcgagaat ttcggtggac ggggttcccg
    13501 gataggcgca ggcgaccgtc aggcctgctt cccaagcccc gcgtgccaat gcctcattgc
    13561 cggagagaaa tgccttctga acagatgttt tttttgccat agtgttcctg ccggtttctc
    13621 aatgcctctg cattctggtt ttaagctgtt aagcgtaccc gatttggccg ccggggtcaa
    13681 ggatgcaaac agcatttttt accggcaaaa ttacaaaatg cccctgctgc ggcgcagaaa
    13741 aaaggcccgg catacatacc gggccgtaag gagatcaaat acgcttgtag atgcgtgatt
    13801 tgactttcag aatgtccatt caacaccgat acgggcaaag taggcatcat cctgactggg
    13861 ctcctcgtaa tagctgcccg gaatgaagta gtcaatttca aacagcgtcc ggaagttctt
    13921 gcagcagctg taggtagcca cggcgctgat catctggccg cggaaattat tgctgccgtc
    13981 aaaagtcaag gcattctgcg gaccgcgggc ccccgtaaag gtattctcat cagcccacat
    14041 caggttgtag tcggtagcaa tcgtccacac agcggtgggc ttaaagctgt gtccgaatcc
    14101 gaggcggtgg agattggtca cattgccgat gccatcgcct tcaaaggtcc acagatacga
    14161 ctgcaggtcg cggcccctct ggaactgcgg ccagtcgccc cagagcgaat caaactgctc
    14221 atcggcgctg gtttcgggat catcgccgga aaggtattca tagccgacat gcagttcgtt
    14281 attccaggag tcattaaacg catacatcag cttgctgttg ccgccaaggg cctgcagggt
    14341 atcgccttcg cggtcgcccc actgcttggc cacttctgcc gaatagctcc agtggtcatc
    14401 cagcttgccg aagaagcggg tgccgactgt atgaatatcc gcatccacgc cgtagctggg
    14461 tcctttatag tgattggccc agtcggagct gtcatcgttc ttatagatgt aatacagctc
    14521 gttggacagc gtgtcgctga gcttgttggt ccagtaagcg ataaagccgc gttcatcatt
    14581 attctgtgtc agatgccgcc ggtcctgcac tgcgtgatga ttgaacggtt tgatgtactg
    14641 ctcctcgtca tcgtactgct ggatatagat caaatcgagc ttgctggtgt cggtcaggtc
    14701 gtactgggca cggatggcat caaagaacat cgtccgtgag ccgtcgccgg gcgtgccgtc
    14761 caagaccagc cagccggtcc ctaagacaat ttcctgacgg ccaaccgtca gcgtcagggg
    14821 caggtcaaag gcgttgcgga actgcagatt cataatgtca aagagggcct catcaaagtc
    14881 gtaattcttc tcggtaaaaa aggaggtaaa ggtatgatcc ggtgcacaat gcccccagaa
    14941 ctcccacgtc agacgggtat tgaaggttac ctcatcggat actgcccact tggccgacaa
    15001 ccgggtgcgg taccgctgcc aatgaaaatt gttccagtcc tgtccgatgc catcacccca
    15061 tgtttcgttc aaaccaaaca ggttgctggt atagacctcg cgcaggcgaa tatccagccc
    15121 catctccacg ccttccatcg ggtgatgaaa tttgtccatc cagtccgtat cgatttcatg
    15181 ccagtgcttg gccggctcag ctgtctgggc agccatcgca acggcagcgg cagcaacaaa
    15241 gacggttaag accaatcctg caaacaactt cccttttttc atgctgttct cctttactaa
    15301 aatcctgaaa atttctgtct gcaactgacg gctatattcc tttgaaacat ttatccagat
    15361 gctccttgga cggcggctgg gtcaaggcgc tgacaacaat ggcggcaaga aacgaaaccg
    15421 gcagcgccac cagcagcgga tccaccacat cccagttggg acaatcggca agaatcgagg
    15481 tcttgccgcc ggtaatccac tggaccagac gcatcgattc ggcttctttg gccttcacca
    15541 gcagcagcca gaaggccgta ataataaagc caatcagcat cgaccagatg gcgccggctt
    15601 tggtcatgcg ccggaagtaa atggctccca gcagcgacgg cagaaacgcg gaggcacaca
    15661 atccgaaaaa gatggatgtc gcacgggcaa taaaggtgct ctcacgtgca tagaaactga
    15721 taatcacagc ataaataatg ccaatgatga tgccgatgcg cgtgatattg gtgctggctc
    15781 cgtgagcgcc ggtaatctgc tcatagacat cccgcccaat gctggtgccg acggtgtgaa
    15841 actggctgga cagcgtgctc atggccgccg acagcagcgt cagcagaaac agcaggccga
    15901 accatttggg catggcgctg acgatatagg ttggaataat ctcatcggca gtgccgccgg
    15961 catagacgat ggaaatgctg cggccttgac ggatttcgac cggctggccg tcgatttctg
    16021 tgcgatccac cgcctgaccg aatagcttga cgggggcgat tttttcgggc acatccgccc
    16081 agacgccggt gtcgcccttt ttcattattt gcaggactcc caatcccttt tcggcatcga
    16141 gcatttttac cacgcggccg ctcaagggcg ctccgtgttc tgaaaaatag gcattggaca
    16201 gcgccccgac agtgaacgct accccggtca tgaccagaat aaacacgccg ccgatcagca
    16261 ccgcacggtt cagctctcgc ctgcttttga ccgtcatgaa ccgcaccacc agctgcggct
    16321 gtgccagcac gccgataccc acacccaaca cgatggtacc gatgacggtc caccataaat
    16381 cgtattggcg gtcgccccag ccgaaggccg gcatggccgt ccagccgcga tgccctatag
    16441 cagacagttt gcccggaacc agcggggcaa gagcagaaag cttctgatgc ccttcaatca
    16501 ctccccctac ctttacgtag ccgtaaatca gcaaggcaaa catcccgata aacataatcg
    16561 aaccctgcag ggcatcggag tacatcactc ctttaagacc gccgacaatc acataagcgg
    16621 caataatcac cgaaaacagc agcagcgctg tcggatactc catattgaac gacttggcga
    16681 caaaaacgct gccgccgatc agcaccgccg cggcatacaa gggaataaag agaaaaataa
    16741 cagtcccggc aaaaacctga ataaatctgc tttcaaaccg gcgtccaaga aattcaggga
    16801 aggtatgcgc atcgagatga tggcccatca ttcgggtacg gggggcaata aacacaaacg
    16861 ccagaaaaat cccaacaaag atgttgcaga aggtcagcca cagcaagctc attccaaaca
    16921 gccccgccac gccgccgaat ccgacaatgg cactggtgga gataaaagtc gccccatagc
    16981 tcatcgccat cacaaagggg tgaacctgcc ggccagcaat taaatagtcg gcggcgcttt
    17041 tggtgcgcat ataccctaaa attcccagat aaacaaccaa aagaagataa atcagaacaa
    17101 caataatatc ccaaagacca acagcttgcg ctaacatata aacctccatt attgtagaag
    17161 agccaacagg caggaattct tacaactcct gttcggtttt cttttcttca gccgcccact
    17221 tgatatcatc cggatggaca gattcctccc ccctattcca gtttatcagt ccccaaatga
    17281 cacacagaag tgtacttgcg atgcataaaa ggtaggccgc taaaactcct ttgtcctcaa
    17341 tcccgagcat aacatctcct tttcacaatg ccagacccac aaaaaaaaga cattaagtgt
    17401 cttctaatga atgtttctgt gaataataac ctctttttgc cgaaaatcaa aaaaaatact
    17461 gattttgtat aattttcgtc atttttgtgc aaatcaggtg aattttttca cattttttga
    17521 tttttataca tatgcaaatt cacaacaaat gcatctttga acactattct tcaagcagac
    17581 gagcagcgtt tttataaaag aacttttcgg caatatgagg cggcagattc agctttttat
    17641 aggactccat tgtttctgac tgatccgtcc aaggggaatc ggttccaaaa agaatgtggt
    17701 cttccgaatg ccgcatcagc atctgtttgg ttcggcttgc cccaaggtat tctatggacc
    17761 aagaaacctc catatagatg ggtttgccca gcagcaggtt ttctacctcc tcccactgct
    17821 gccagccacc cagatgggtt gcaataagct gcagagacgg aaacctttcc agaagtgtaa
    17881 ggatttgccg cggcgaggcg cgttcaatcc gctcaaaagc gatgtcgtag ccggtatgaa
    17941 cggtcacaag gagtctgctg tcgcagacag cctgccaaaa cggaaacaac gctgaatcgt
    18001 ccagaacaaa cgactgatag taggggtgca gcttaagccc cctgaagccg gcttgggcaa
    18061 tctgtgccac tcgttcgacg gccagcggat cttgcggatg aaccgaagga aacgggacaa
    18121 tgcggtcgga ggcaatctga cagcaccaat taaaaattga ctcgaactgg acagggcggg
    18181 ttgcaatgca gcacaatacg cttttttcaa tcccgttggc atccatcgat ttcagtagcg
    18241 aggccagttt gccgtctaaa aaagagcgaa cctcggcaca ctcggccttc aattgcgcca
    18301 tcgccctgtc ggccagcgca tcgggaaatg cgtgggtatg aaaatcaatg attttatccg
    18361 tcatcgcacc aatcctcctc gtacggatgc aaatcaaaaa aaaaggcccg gcgttagttt
    18421 cccgggcctt cgaattcaca gaacaagcac gtcagcaggg ttttacctgc gttgcttcca
    18481 ttcctttttt gccctgtgcg ggttcaaact ggactttctg gccgtccttt aatgtccgaa
    18541 aaccctccgc aatgatattg gagtgatgca caaacaaatc agctcctcca tcctccggaa
    18601 tgatgaaccc aaaacctttt ttctcgtcaa accacttaac cgtacccact gccataaaac
    18661 tactccttgg gcttgcagcc caattgaaac tatttccctt cacttttttt caagagtcgt
    18721 cccaaaggga aaaaggcgac tccttataac tcaaactcaa ttagacctct atagtcttga
    18781 aaaggcaaac aaaattttca taattttttt tatttttctc tgtcctgcct taacaggcag
    18841 gcaaaaactt tcggtcgatt gcccattcta ctggatatcc caaccggttt tggaacgcat
    18901 ttttttcaat tcagaacgtt cttttttatt gtgcagccgg cttaagattg cctgccgggg
    18961 gacagctgtc ttttttcgca cccgcaccgg tttgagagcc gatgcgatca tctgtgctat
    19021 ttttttcatt gccgccctgc ggttttccat ctggctgcgg tattggctgc aggatacttc
    19081 caaaacaccc ttcttatcta ttctgccctt ccacattgta tataaacggt ttttttgatt
    19141 atccgacaga caggcacatg ccggaatatc gagaaaaatc tgcacacggg tattgagctt
    19201 gttgacattc tgaccgccgg ggccgctgct gcgggtataa aaaatcctca aacagcgagc
    19261 aggaataata agctgcggat taacagcaag gtcttccatt atttatggga cgaagtcccc
    19321 cgacagcatc tttaattttt ttcaaagtat aaatcgatat cctttgccgt gtatggtctt
    19381 aatcagcgta cgtgatttgc cgagtttttt tcgcaatgcc gcaacgtgta catcgacaat
    19441 acgtgcgttg gcgttttgtt cagccggagc tgcctgtttg agcaattgtt cccgcgaaag
    19501 aatgtttccc cccgccttga gcagtgccag cagaatattg aactcggcgg gggtcaaatc
    19561 cacaggattg ccctcgacaa atacctgccg ctgctctgga aagactctta ccggtccggc
    19621 ttgcagattt tccctgacgg ctgctgtgtc agctggatag acccgccgca ggacagcatc
    19681 catccgggcc agaagcacct ttgtgctgaa cggtttggtt atatagtcat cggcccccac
    19741 acttaggccg acaataatat ccgtttcgct gtctttggcc gtcagcagaa taatcgggat
    19801 gcgggcagtt ttggaatcgt ttttcaagcg gttggtcagc ttgatgccgg aaatatcccc
    19861 cagcacaata tccagcagaa ttaaatccgg cggctctgcg gcaatcatcg acaaggcctc
    19921 ggccccgctg gcggctgtcc gcacgatata cccttcctga ctaagggcta tactcagcag
    19981 ttcgagcaaa tctctctgat cgtcaacaac aagaactttt gcgttcatgg aaaaacagat
    20041 ttttcaagaa attaacagta aatcgctgtt aatgtatcgg tatttgctgg atgggactgt
    20101 aataaatcat cgaatgcagg acagcattta acggcattct ggagggatta agcatttaat
    20161 ttgcgattta agatttcact tacaattttc ggattggcct gccctttgct tttttgaatg
    20221 acctgtccca tcaggaatcc cagggccttt ttggcctttt tcggattgct tctggcatct
    20281 gcgacggcct gaacattttc tgccagaacg gcatccacaa gggtctcaat ttcgccggca
    20341 tcgcttttct gaaggagatt tcgcttttca gcaatctgca ccggatcgcc gccatgggcg
    20401 gcgatttcct cgaagataac cgccgccgaa gttgcactga ttaaacccgc atcggtcatt
    20461 tgagccaatt gggccagctg ctgagccgac aagcccagct ggtccacccg gatgcccctt
    20521 tcattggcca gcttcaggcc ggtctgggtc agcaggttgc agatgcgctt gggatctgcc
    20581 tgcaatgccg ccgctgcatc aaaaaattct gccgtcgccc gatcggctgt cagcacagcg
    20641 gcgtcgtagg gacttaatcc gtatgcctga ataaatcgca tctgcatctg cagcggcagt
    20701 tcgcacaggc gtgcttgaat cgccgtcagc cacgcctgat ccacttcaac cggcacaagg
    20761 tccggatcgg ggaaatagcg gtagtcatgg gcttcttctt tttcccgctg gagaacggtt
    20821 gtctgccgct catcgtccca gccccacgtg cttttgttgc ccatctgaag cgttcggcct
    20881 gtctctaaaa agtcctccag ctgccgctgg acttggcagg caatactgcg ttcaatagcc
    20941 cggaaactgt tgaggttttt gatttcgctg attggggttt tgaacagctg gccgttacgg
    21001 gtaatatgaa gattgacatt cggctcgaaa cgcatatgcc ctttctgcat atcggcctcg
    21061 gaaaccccca gaaagcggac gatccgctga agttcgcggg ctaacgcacc gacctcttcg
    21121 gggctgttca tatccggttc ggttacgatt tccagcagcg gggcaccggc gcggttgaga
    21181 tcgatggcgg aaaagttgcc gaagctgtgg acgttcttgc cggcgtcttc ttccagatgg
    21241 acacggcgga tgcggatttt tttgggctgg ccgtcagcag ccggaatttc gatatatccg
    21301 tgctgggcaa acggcaggtc atactgactg atttgataat tcttgggcaa atccggataa
    21361 tagtagctct tgcggtccca tttggtaaag cgggcaatcc ggcagttcag cgccagccct
    21421 gtcagaatag caaactccag cgcctgctta ttcatcaccg gcaatgcccc cggcagcccc
    21481 agacagaccg gacaaacatg cgtgttggcc gcatcaccgt aggccagccg gcagccgcag
    21541 aacatcttgg tctgcgtggc cagatggacg tgaatttcca atcctacgat tatcttgtat
    21601 tccatgggct gtttccttta ctgccgcagc gatttcaaag ggggcctgca ccgatgccag
    21661 ccggtttctt tttcatgcat tcgggcaatc cgcagcagct tgtcttcggt aaacggcgcc
    21721 gagatgattt gcaggccgac aggcagattc tgccggtcaa atccacaggg gatactgatg
    21781 cccggcacgc cggccagatt gacggcaatg gtataaacat ccgccagata catctgcagc
    21841 gggtcatcgg ttttttcgcc gattttgaag gccgtggtag gactgacggg catcatcagg
    21901 cagtcggcct gctcgaaggc cttggtaaaa tcgctgcgta tcagattgcg caccttgagg
    21961 gccttgaggt aataggcatc atagtagccg ctggaaagag cataggttcc gagcataatg
    22021 cggcgtttga cttccggccc gaagccctca tcgcgcgagc gggaatagac ctcgatataa
    22081 tcgccggcgt tcgggctgcg gtagccgtaa tgcacgccgt cataccgggc cagattgctg
    22141 gaggcttccg cagtggcaat gacataatag gcgctgatgg catagtcaaa atggggcatt
    22201 gcgatttcaa ccagccgcgc tccgagcgat tgatagactg ccagtgcttc ttgtacagcc
    22261 ttctgcaccg aactgtcagc cccttccagc agagagggga caacggcaat tttcagccct
    22321 tcaacgggct tttccagctg tgccgaataa tcacagacag gaatgtggct gacggaggtg
    22381 ctgtcgcggg gatcccagcc ggcgatgacg cccagcagca gcgcagcatc ggccgtatcc
    22441 tgtgtcagcg gtccaatctg gtcgagactg gacccataag ccaccagccc gtagcgcgag
    22501 accctgccgt aggtgggctt gagtccgaca acaccgcaaa aggatgccgg ctggcgaatg
    22561 gaaccgccgg tgtccgaacc cagtgccgcc ccgcacaaac ccgccgccac tgccgcagcc
    22621 gaacccccgc tgctgccgcc gggcacacgc gagatatccc atggattgac cgtgcgcttc
    22681 aggccggaat tttctgtgct ggaacccatc gcaaactcat ccatattggt tttgccgata
    22741 atgacggcat cggcctgcgc cagcttctct accacatggg catcgtacgg ggaatgaaag
    22801 ttctcaagaa tcttcgagcc gcaggtggtc gttccgaagg cggtgcacat attatctttt
    22861 atcgctatcg gcacacccgc aagaacaccc gcggcttgac cggcggcaat ttttctgtcc
    22921 acagcctcgg cctgctccag cgctcgatcc tgatacaggg aaagataggc cccgataacc
    22981 ggctcgtaca tctcgatgcg ctcaaagacg gcctttaccg cttcaacgct tgtgatgtgc
    23041 cgggccgcaa tcctgtctcg aagctgggtt aatgtatcca tatccttatc cgtttatctg
    23101 gactttgaca gaagccactg atagagctgg tcattgctgt aggtctgggt ccaggaatcg
    23161 tggtctgcct ctggatagac agtcagtttt gcctgtccgc cggcccgatt gaccgcatcc
    23221 accatttcct gcgactgctg cacaggcacc acagaatcct tggcgccgtg aaaagcccac
    23281 acaggcacat ctttgaggtt tgcggccaga tacggcagac ctccgccgca gatgggggca
    23341 atggccgcga aacgctcggg atacatgcag ccgattgccc atgtaccgta gccgcccatg
    23401 ctcaaacccg tcagatagat gcggcgggta tccacaggat agcgttcggt gatttcatca
    23461 atcagggcta tgacggactc ggccatgtac ggccaccatt ttccggcggg gcactgcggt
    23521 gaaacaataa taaacggcag gtcttttccc tgctccacaa gcttggccgg accgtggact
    23581 ttgacgcggc tgacatcgct gccccgctcg cctgcaccgt gcaaaaacac cagcagcggc
    23641 cagtttttct gctctttccc gtaatcggcc ggcaagtaca gcaggtaatc caattgaaca
    23701 gccgtctgcc gatgcagggt ttgaggatgc tgccccggct gggttattgt ctgtgcctgt
    23761 ctgctctttc ccgtgcatcc gctcataatg ctcatccccg ctgcaaccaa caatgctgtt
    23821 ttgatgttca tagaccgtct ccattctgcg gctcacgcgc cggaactgtc atccaaaatc
    23881 cggggcactt taaagtattc cccataccgt tccggagcat tggccaatgc cgcttcattc
    23941 ggcagtgaag gccgaggctg gtcatcgcga aagacattat gcaccggcag acagtgcgcc
    24001 agcggttcga cgccgtcggt attgagttgg ctgagctttt caatgtacgc aacaatggcg
    24061 ctcagctgaa cactgaactg gcggacttcg tcatcggtta aatccagtcg gcttagaagg
    24121 gccacccgcc gtacctgatt ctggtcaatc ttaactgcca tcggcttgtc cctgtttgct
    24181 gaattccggc aattttactg caatcggtca ataaaaaagc gagactgttc aaacagcctc
    24241 gcttgacgtg cgaatcttaa aatacagccc tctccggcgc gttagctggc tgcctgttgt
    24301 tctggtttcc agcggcgttt ggggggtttg acaaccagtc ccatccgaat ggccttggcc
    24361 gaaacgcgga gccggcacgg tttgccgtca acaacagccc gcaccttctg tacattggcc
    24421 ttgaatttgc gtctggtgac tcccgttgtt ttaataccca cgccgcccag atatttggcc
    24481 ttgccccggc gggcaatgct gcggccggtt cttgtttttt tgcctaaaaa ataacattct
    24541 cgtgccataa caaactccca attgattctg cctctatcca gcggctgata aactttattc
    24601 caaagcccca caaatataga catttgctat gctttttcaa gtccttttta cagatttttt
    24661 tgcttatcct ctcattttaa gctgcaaaaa ttcattttta cttgattttc aaggttattt
    24721 tcctactctt gcacggaatg gaatccaaag cagaagactt aaataaaacc aaacgcgtcc
    24781 tgattaccgg ctgctccagc ggttttggac ttctgacggc tgttgaagcc gccaaagccg
    24841 gctttgaagt aatcgccaca atgcgaaatc tggacaaaag ccatctctta cgagaagcac
    24901 ttcaatcggc cggcacagca gcccagattg agcaattgga tgttacaagt ccaagttcaa
    24961 ttaaaaacct gacagacaag gtaggatatg tcgatattct ggttaacaac gcaggcatcc
    25021 tcattatggg ttcatttctg gaccaaacag atgccgaaat gcggaagatt tttgaaacaa
    25081 actatttcgg gattgtcaat cttaccaagg cggttgtgcc ggcaatgatg gcacgcaaat
    25141 ccggtctgat tatcaatatc gcctcgctgg ccggtttggt ggggcatccg ttcaatgccg
    25201 cttatgccgc cagcaagcat gccctcatcg gatttaccaa atctatccgg gtggaattag
    25261 ccccctttgg aatcaaaacc gtatccatag agccggggta tcaccgcacg gaaatcatcg
    25321 gcgccaatgc caatcagtca gaaaacttct acaatcgcca aagcccaatg tttgaatgga
    25381 atcggggttt tctgcgtgca atgtttgacg agattctccc tcgtgccggc catccgcagg
    25441 cagtcgcaga cctgattgtg cggatcatga caagtgaaaa aatcaaaacg cattatgtca
    25501 tcggcaggga tgcacggttt gccgctacga tgcaatggct gggccttggt ccgtggctgg
    25561 agcggaaaat ctgccgaaaa atcaagcgga ctacccgttt ggaaaataag cgccaagcag
    25621 agaagaaagc ccgacgaaca caaaaagcat ctgagcagtc ctgattatgg ccaaactgaa
    25681 actgctgttg gtcaacccgc cgatttacga ttttacggcg tatgatttct ggattaagcc
    25741 ctacggcctg ctgcaggcag gcggtctgct gcgttcgatt ggccagcttt ggctgtttga
    25801 ttatctggat cggctgcacc cggcctttga taaggcagtg tctgcaaaaa ccgatgcctg
    25861 cggactgggt ccttatccgg aggttaaaat ccccaagccc aaaccgctgg acgatatccc
    25921 tcgctattac cgccggtttg ggatgccgcg gggtgtcttt caggattatc tattccgcaa
    25981 cggccctttt gatgcggttt tgatacaaac aatgatgaca tactggtatc ccggtgttcg
    26041 ggaagtaatc gacgatgtcc gtaccatcag tccgggggca aaaattattc tggggggatt
    26101 ttatgccgtc tgctgtcctg agcatgcgcg cgggctggga gcagatgcag tcgtttcctc
    26161 cgacgacctg acagctcttt ggcagacact cggacagacg ccgccggcag aaacctgttt
    26221 gccggcttgg gaactgtatc ccagactgca tacggctgtt ctaaagctga cttcgggatg
    26281 cccttttcat tgttcctact gtttcgtgcc ggtcagccgg acgccattta agatccgccc
    26341 gctggaagac tgccttgccc aattgcggca attggtcagt ctgggcgtcc gaaacatcgc
    26401 attttatgat gatgcgctgc tgtaccagcc ggaaaagatc ctgcttccgt tcctgcgtta
    26461 tatagtcgaa aggaaaattg gcgttcattt tcataccccc aatgccctgc atgcacgctt
    26521 tataaccgcc gaagtcgctg aatggatggt gcgggcagga ttcaggactt tttatctggg
    26581 ttttgaaagc cgctgcgaac tgttccaccg ccgcacgggc tcctgcaagg tcgccggcaa
    26641 cgaactcgaa caagccgtcc agcacttgct aaaagccggc gccaaagggc aatctatttg
    26701 tgcttacgaa atgttaggac atccgcaaac cgatattcag cagcttgaag attcgatgcg
    26761 gtttgtccat agtcttggca tccgaattat gctgtcggat ttttccccga ttcccggcac
    26821 gccggatgga gaattatgcc gcacatatac caacttggat gagccgctta atcacaataa
    26881 aacggctttc cctatccgct atttaggaat taagaaagta aattactaca aggacctgtg
    26941 ccggagattg aattcctatt tgcattgatc tgaacaagag aaaaagccaa tataaaaata
    27001 attttttctt tttttaaaaa atttttttgg aaagaaaaca ttacattagg gtatatgata
    27061 attagtagta ggataaattc ctatttgcaa gattttgcgg ttttaggatt taattgctgg
    27121 aaaaagtgga gaattataaa tgaaaaaaag atccgctttt actcttattg aactgctcgt
    27181 tgtaatagcc atcattgctc ttctgctcag cgtgattata cctgccttgc gaaaggcaaa
    27241 aacgcaatcc aaaatggtcg tttgcagaag caatatgaga ggtgtccaga caggtctgct
    27301 gctgtatctg gaagccaatg accaaaagat gtttccgtac agcggctggc tttggataaa
    27361 gaaactggaa gcccacgtcg gcgacttgga cgagatacgg tattgtcctg aaattgttgc
    27421 aggcgtcgat gcggcgcttc aagcagccca gacagccaat gccaaccgct ggggaacctc
    27481 gcgtcagcca tgggtctggc ggcagacaga tcctgtcacc ggacaaatgc tttatgaggc
    27541 cgggggatta ggatttaacg gctggctgta tggaaatgta aatacatggg tgccggcagg
    27601 catggaaaac tatccataca aaacgatgtc cgaagttaca agccccagca aaacacccgt
    27661 ttttgccgat gaaaactggg ttgattcgtg gccgcgaaat acaaatcaac tgcatcccaa
    27721 ccttctgtcc gatccgcaaa cctactacaa cacgggggat accacaggcg gcacatccga
    27781 ctacgcaatg ggacgttttc tggtcaatcg gcacttcaat ctgcgaatta atatttcctt
    27841 cattgatggt catgtcgaaa tgattgatat gaaagacctt tggacattgg cttggcatcg
    27901 cggagcaaag ccgaattttt ccattaccct gcccaaagcg aaaaactaac gcagctttga
    27961 aagataccgc cctgtttatt tcaataggca gttcttgtat tgcatcattt aaggtattat
    28021 atatctgctt atgctttata ttcagcgcgt ttgcaaattt tcagcaaagc atctgcttgc
    28081 tttcttgcag ataagcctat ataatggttt agcaaacgct ttataaacaa tcatactgcc
    28141 aaaaaccgga gactataaaa atgaagaaac attctgcctt cacattaatc gaactcctcg
    28201 ttgtaattgc cattatcgct ttgctgcttg ctattatcct gccgggtttg cgggctgcca
    28261 aggaattagc atccggcgtg gtctgtcaga acaacctcaa acagctgaat ctggcatgga
    28321 catcgtatta taacgacaac aacagtctgc tggtgggcgg ttcaaattat tacagcggga
    28381 cacgggccac tccctaccgc tgggttgagc cgccgctgct gaaagacaca gacaatcccg
    28441 aatttgtggg cattgcaccg atggctcagt attcgcttca gacccgcaaa aacgggattc
    28501 gtgcaggcaa actgttcccc tacacgcagg atgaagaatt gtatcactgc cccggcgacc
    28561 ggaatatcac caagctggaa cccgaagcta tttaccgaag ctatgccatt acaggtctaa
    28621 tgaacgggga agactttatt gaccgctacg cgggcctgta ctcgccgata cgaacccagc
    28681 gaaccgctat tggaaagacc ctgaatgtag taattaagtt ttcggaagtc cgcgctccgg
    28741 gcaacaaaat tgtctttatg gaagaagatg tcgccagcaa agctacaaag cagtactata
    28801 atgcaggcgg atttgtttta ttgggcggcg gagcgtttgc atggtgggat tggccggcct
    28861 attaccacaa cgacagcagc accattggtt ttgctgacgg tcatgccgag cggcatcgct
    28921 ggcgtgattc cgatacgctg aatctgataa aggaaggcaa ggcggatccg gatccggtca
    28981 ataaccaaga cctgcaatgg ctggtacagg ggtatatgcc caagccctga ggctcatcca
    29041 cacattcacg aatagcagat gacgagagat gaagaatgaa aagaaatcgt gcctttacac
    29101 tcattgagct gctggtcgtg attgccatca tcgccctgct gttaagcgtg gtcattcctg
    29161 caatgaagaa tgccaaacgg cttgcggcag cggctgtttg tctgtccaac gaagggcaga
    29221 tggttaaagc gtggcttatg tatgccgaac aatatgacag caaatttgtc gacggcgata
    29281 cgtccgatga tttagtccgg ccgggctaca ccaactacgg aagtggatat gtctggaact
    29341 gggttggacg gcccatggga ccggccaaac aagatatcaa cgacacgctg gaagacaaat
    29401 tccggggctt cgaggcagga ggtttatggc cgtttattca ggcgccgaag gcctaccatt
    29461 gcccggcgga tttgcgccat ttgagacgtg ccgtttatcc ctataacagc acaaccggca
    29521 atcctccggg attgtggatc ggcggctatc ggacctattc catcggaaag gttttgtctg
    29581 tccggcccgc aagcggttcg ggagaagacc gggttactgt ttcaaaagta tcggaaatca
    29641 ttaatacatc cgcaaagatt gtctttctgg aagaaaccga cggctacggc tggaatcacc
    29701 gaacatggaa tatgaatctg ctgagcccgc aatgggtgga cccgtttgct atcctgcaca
    29761 acgacagcag cacattcgcg tatgcggacg gccatgcaga ccgacacaaa tgggtggacc
    29821 agcagacgcg ggatatggcc aaagctcagc agaagggctg gtcggcggtg gatcccaaaa
    29881 ccggctcaac gcaggattac gactggttca agcggtcgta catgcccaaa cagttcaaat
    29941 agttgttaat cccgccgcca tccggcagct gctggccggc agctgtgtac aggattcaat
    30001 atgtgggagc attttcatca tcaggcggat atcggcatcc ggggaatcgg acaaacactg
    30061 gacgaggcgt ttgccgaagg ggctactgct ttgatggccg ttatttgttc gccgcagcag
    30121 gttcggccgg aaaaagaggt tgacatttgc tgccactgcg acgaaaaaga ccttctgttt
    30181 gccgactgga ttaatgcatt gatttatgag atggacgttc ggaagatgct gttcggccgc
    30241 tttgaggtgc acattgaaga cagctgtctc aaagccaagg cgtggggaga accgacggac
    30301 ccgaaccgtc acgaaacggc ggttgaagtc aaggccgcta cctatatgga attgaaggtg
    30361 taccaaaatc cggctggtct ctggattgcc cagtgtgttg tggatgtctg aaaaaggacg
    30421 gtacaaatgg atagaaaact ggttaagcgc cgaggggagt acatatggct gctggagcga
    30481 agcgggccga tgcatgtgga cggcattatc ttcgcttccg aagaattaat cagccagatg
    30541 gatgatatgg ttgcccagca ggttcgcaat gtcgcatcgc ttccgggcat tgtggaaggc
    30601 tcgtttgcaa tgcccgatgc acattggggg tacggtttct gcatcggcgg cgtagcggct
    30661 tttgaccctc aggaaggcgg cattatcagt gccggcgggg tcggatttga tattgcctgc
    30721 ggggtgcgta cgctgcacac agggcttaaa gaagaccagc tgcaggggct gtgcccgacg
    30781 ctggcggaat cgctgttccg gcatattcct gcaggcgtcg gcagcacagg cagcctgcgg
    30841 ctgaccaaga gccgtttgga tgaggtgctg gccggcggcg cccgatgggc ggtggaaaac
    30901 ggattcgggg acaaagacga cctgcgcttt atcgaggccg gcggctgcat ccacggtgcg
    30961 gatccggcct gcgtttcgga tgaggccaaa aagcgtcagc tggaggaaat gggcacactg
    31021 ggttcgggca atcattatct tgaaatccaa aaggttgttg agatttacga caagccgaca
    31081 gcggaagcat tcggaattca ggaaggcgat atcaaagtca gcatccattg cggctcgcgc
    31141 ggattaggac atcagattgg aaccgattat ctcaaaaaaa tggcacaagc ggccaaacag
    31201 caccatatca accttccgga gcgggaactg gcctgtgcgc cgctgtcttc ctcggtcggc
    31261 aggcagtatc tgggggccat gcggtccggc attaattgtg cgctggccaa tcggcagatt
    31321 ctgacgcatt tggtgcggga ggtttttgag caagtctgcc ccaaagcaga tatccgcctg
    31381 ctttatgatg tatcgcacaa cacctgtcag gaagaagaac atcccgttga cgggacaata
    31441 aaaaaattgt ttgttcaccg caagggagcc acacgtgcct ggggaccggg ccgcagcgaa
    31501 ctgccgtccg aataccggcc attcggtcag ccggttctga ttggaggtac aatgggcaca
    31561 agctcgtaca ttttggcagg caccgccgaa ggccggcggc tggcgttcga atcggcctgc
    31621 cacggcgccg gacgcagcat gagccgcaac gccgccacca agcagtataa cgccaaagac
    31681 atcatcggcc ggctggcgca tcagggcgtt ctcatccgca gccggtcgat gcggggcatt
    31741 gcagaagaag ccccgcaggc ctataaagat gtcagccaag tggtcgaagc cacccaccgg
    31801 gcggggctgg ccagaaaggt cgccaaactc atcccgcttg tctgtgtcaa gggataatta
    31861 tcccgcaaaa caacgaccga aaacgccgat cgccttctgc cggcaagaga ctttacgcag
    31921 tccaatctgt ctgcctgtgc ggatttgcat tttaaggtct ctgcgccggc cggcggctgc
    31981 tgcttgacag ggccttccgg ttcttgtaga ttgcctgtct gccaagatgt gttggcttcg
    32041 gcataaaaga aaggacaccg tctattgaag cgtttgcgaa atgatatgta tgcctgcgga
    32101 cagtcgggac ttccgcaggc gatttactta aacggtcaga aatggacatt ggagaccgtg
    32161 ttcaaacatg attttttcgc ctgcaccggc cgctatcaat gccacctaag cacggagaag
    32221 gttgttttga aaatcagccg cctgcagtct tttttcggta ttccgtgtgc atggctgggg
    32281 cggatgctgc gcaatcacga attgactatc ctgcggcatc tggcggatat tgaagaggtg
    32341 ccgcatatca tcaggtcctt tgggcataat gggtttcttt attatttcat tgaaggccaa
    32401 tcgctcgatg aaaaaccggc tgtgccggat gactttttct cacggcttcg tgcgctgctt
    32461 gctgctattc acaagcggga tgtttgttat atcgacctca acaagcgggg caatattctg
    32521 ctgggcaaag acggccggcc gtatctgatt gattttcaaa tttcactgca tttgcccggc
    32581 aggtggtgcc gctttctccg caccgccatt cagcgggagg atatgtatca tcttctgaag
    32641 caccatcgca aatttcgtcc ggatttgctg actccagaag agacccagcg ctcgcggcag
    32701 cccagcggcc tgattcgcct gcaccgttta gcggcgcatc cttatcgaca gattcgaaga
    32761 gccctgtttc ggtttctgta caaaagccgt attttaactc cggatgacag ccaccatcgt
    32821 acgcccgaaa acgaccctaa ccgttttctc cagtaatgct tgctgccggc aaaacacgcg
    32881 acaaaacatt tgctttgagg tctttttttg gatataattt aacccgattg gctttcgggg
    32941 aaaggaattt acccattgaa tctatccagt aaaaatcagc tgcaggggat gatttttgac
    33001 atcaaaaagt acgccattca cgatgggccg ggcattcgta caaccgtctt tttcaagggc
    33061 tgtccgctgc ggtgcagttg gtgtcataat ccggaaagct ggtcggacaa gctggaactg
    33121 atgtttcgtg ctgcccgctg tgtacggtgc ggccgctgtg cacaggcgtg tccgcacggg
    33181 gccattcgga tggacggcct tcctgttacg gatggctcag tttgccgcca atgcggagcc
    33241 tgtattcctg tttgcggaca gcaggcgcgg gaaatcgccg gcagaaaggt cggtgtcggc
    33301 cagataatgg acgaaattct caaagatgtc attttttatg atgactccgg cggcggcgta
    33361 accttctcag ggggggaacc cctgatgcag ccggaatttt tggaagctct gttagaagaa
    33421 tgcagaaacc gggagattca tacagcagtc gatacatcct gctttgcacc gcagagtgtg
    33481 ctgaaaaaga ttatcccgct aacagacctt tttttgtgtg atatcaagca ccttgattct
    33541 gaaaagcata agcgttttac aggtgttgac aacaagcaaa tcctgacaaa cattcgcttt
    33601 ttagtcgaaa acggctgcac catcattgtt cggatacctg ttatcccggg ttttaatgat
    33661 acggctgagg cggtggatgc gattgcccga tttgtaaaaa cgatgaacac tattcaccag
    33721 attgaccttt tgccgtataa tagcggaggc gtatccaaag cccagcgtct tggcggcagg
    33781 atccagattc ttcacggacg ccggcctgat aaagagctga tggctgaact ggaaaattgt
    33841 gttcgacaat acggattaaa aacaaccata ggcggatgat tatgaacgag cgggtacgaa
    33901 aactgcgtga ccaaagtgta aatacaaaac cctatatcag tgcggaacgg gctgtgctga
    33961 taacggattt ttaccaaagc ggaatagccc tgcaggtatc tgcgccggtc ctgcggggaa
    34021 tggcgctgaa gcatattatg gagcacaagt cgctgtgcat caatgacggg gagttgattg
    34081 tcggcgagcg gggacccgcc cccaaagcca cacccacgta tcctgagctg tgctgtcata
    34141 cactggaaga cttcgaggcg atgagccggc ggcagcgtac gtcgtttgat gtcagcgacg
    34201 acgtcaaaca ggtgtatgca gaaaaaatca tccccttctg gaaaggcaaa accatcaggg
    34261 aaaaggtgtt tgcttcgatg cctgaggcat ggcacaaggc ctttgaggcg ggtgttttta
    34321 cagagtttat ggagcagcgg gccccggggc atgccattct tgacgataaa atctacaagc
    34381 gcgggctact ggatcttcag gatgatatac gccagtcgct gcgtcagctg gattatttca
    34441 acgatccccg tgcgtatgaa aaagagcagg aattgaaggg catgagtctg gccattgatg
    34501 cggtcctgac gctggcccgc cggtattccg aagaatgtgc aaagttggcc caacagcaga
    34561 cggatcccca gcgccgaagc gagctggaga aaatggccgc catctgccgg catgttccgg
    34621 cctatgcccc gcgcactttc cacgaggccc tgcaggcata ctggtttgtg catctgggag
    34681 tcattacgga attgaacgtg tgggattcct acaatcccgg ccggctggat cagcaccttt
    34741 ggccgttcta ccagaaagaa atcgccgaag gcacactgac ccgagagcag gccaaagagc
    34801 tgctggaatg cttctgggtc aaattcaaca accagccggc cccgccgaag gtcggcatta
    34861 cggaggagca aagcggcacc tataccgact ttgccctgat caatatcggc ggggtcgatc
    34921 cgcaaacggg agctgatgcg gtcaatgatg tgtcgtatct gatgctggat gtggtcgaag
    34981 aaatgcgtct ggtgcagccc agcgcctgca ttcaaatcag tgccaaaaat cctgaccgct
    35041 tcctcaagcg cgcctgcaag gtcattcgct gcggctttgg acagccgtcg gttttcaata
    35101 ccgatgtgat tgtccgcgaa atgctccacc acggcaaaag catggtcgac gcccgcagcg
    35161 gaggtcccag cggctgcgtc gagaccagtg ccttcggcaa agaaagctgc acgctgacag
    35221 gctactgcaa ctggccgaag atattggaac tggccctcca caacggcatt gatccgcgta
    35281 ccggcattca ggtcggccct gcaaccggca aggccgaaac atttgacacc tttgaaaagc
    35341 ttctggatgc gtaccggcag cagctggggt attttatcga cctgaaaata cggggcaact
    35401 gcatcatcga gcggatctat gccaacttta tgccggctat gtttttatcc gtcctgaccg
    35461 acgactgcat caagaaaggt ctcaactacc acaacggcgg tccgcggtac aattccacct
    35521 atattcaggg ggtagggctg ggcacgctga ccgatgcgct ggcggctgtc aaatacaatg
    35581 tctatgacaa gaaaaccgta accatggaaa cgcttttaaa ggcgctgaaa gccgatttcc
    35641 aaggatacac cgaactgcac aatcagctgg aagccagccc ccgctacggc tgcgacaatg
    35701 actatgccga tgagctggcc ttgcgggtat tcaatattta ctacgacctg attaacggcc
    35761 gtcccaacac caaaggcggg cactaccgca ttaacctgct gccgacaacc gtgcacattt
    35821 atttcggtca gataaccggc gccctgcccc acggccgcaa ggcgggcttg ccgctgtcag
    35881 acggcatttc gcccacgcag ggcacccgct gtccgggacc gaccagcatt atcaagtcgg
    35941 cttccaaaat cgaccattcg cggacaggcg gaacgctgct gaatatgaaa ttcaatccgc
    36001 aggtgctgga ggggccgggg cttgaaaaac tggggcattt gattcgcagt tatttcaaac
    36061 tcgacggcca ccatattcag tttaatgtca tccgcaagga aacgctgctg tcggcacaga
    36121 aaaatcctgc tgaatacaaa gacctcatcg ttcgggtggc cggctacagc gattacttta
    36181 tcgacttggg aaccgacctg cagaatgaaa ttattgcgcg gacagaaatg acagaaatct
    36241 aatccccgca cagggcctaa aaagccatag gatgaggaga gagaaccggt ctattcgtct
    36301 gtgttctcat cgaacagcaa agcatcgtca tcggccggct tctgttttcg gacgtctgca
    36361 agtccaagat gtcggcaggc ggcggatgta atctcgcggc cgcgtttggt gcggcggaca
    36421 aaaccgattt gcagcagata cggctccacc acatcttcca atgtgtcacg ctcttcgccg
    36481 agagtggcgg ccaatgcctc aatgccggcc ggaccgccgt catagacctt gaccagcgcc
    36541 cgcagaaaac tgcggtctaa ctcatccaaa cccagcggat cgatttgctc catcctgaga
    36601 gcactttcga ctatttcggc ggtgagcttg ccgctgccct tgacctgagc ataatcgcgg
    36661 acgcggcgca gcagacggtt ggcaatccgc ggtgttcccc ggctgcggcc ggcaatcaat
    36721 tcaagggtgc cgttttcggc tttcagctcc agcagtgcag cagaccgttt cagaatttcg
    36781 gtcaattcgc cgacattgta gaattcaaga tgataaagca tgccgaaccg tccgcgcagc
    36841 ggggcactca gcagaccggc acgggttgta gccccgataa gcgtaaaacg cttcaggggg
    36901 aaattaatgg tcttggcgtg cataccgctg tccacagtaa aatcgacctt gaaatcctcc
    36961 atggccggat agatgaattc ctccaccgga gcactgagac ggtgaatctc atcaataaac
    37021 aggacatcgc cggtgttgat attgctcagc agtcccatca ggtcgccctg cttggtaatg
    37081 gccggcccgg aggtaacccg gatattggca cccatttcat tggcaatcac atgggccagt
    37141 gtggtcttgc ccagtccggg cgggccgtaa aacaaaatat gctccagcgg ttcgccgcgc
    37201 cggcgggcgg cctgaatcgc tatggacagc ttgtcgcgga caggggtctg cccgatgcat
    37261 tcggccagcc gctgcgggcg aagagctata ttaaactgct cttctttatc cccgccggaa
    37321 tctgcactta atactctgtc aattgccata ccggtaattc tttatcttgc tcaacggttt
    37381 acacttcgac gccctgcttg atgcggtaca ccagcgggac aagctcttcg gctgttttta
    37441 tttccggatg tttgcggcag gccagctcaa tcagttccat cgcctcggcc cgcttctcac
    37501 cccaagcaat caggatttcc aatgcctcca tctggtaggc actaaaagcc gaacggcttt
    37561 gcgcagcggc ggtttggagc gcataggccg tcagcttgcc tttcagttcg gatgagaccg
    37621 tctgtgccat ttttttgccg attcccggca atgtcgccag cagtttttcg tcgccggact
    37681 cgatagccgt agcaatgcgg tcaatcggca aggccagcga tttcaatgct ttcttgatgc
    37741 ccattccctt aacgccggta taaatatcga aaaagtcgcg ttcgaagcgg ctcagaaagc
    37801 ccaccatccg cgggatgagg ttgccgccgc ccaacgtgcc ttcatagtac tcaaaggtac
    37861 acagcgtaac cggctggccg agccgatttc caagctgcat cacggcatag cccggcagca
    37921 tgacctcata acagaacgat ccggcctgta tcagggcggt gtcttcggtc agggatacaa
    37981 gttttccttc aatttgtgca atcatcgctt ataacctgcg cttatcttca tttatcggct
    38041 ctaaacagcc tgatgttcgg atgcacagca cagggcaatg gctatagcat cggccacatc
    38101 cggcggtttg ggcacatcgg gcagagccag tacagtctga atagcccgct gcatctggcc
    38161 tttgtgtgcc cgtccgtttc ccgtcagcga cttttttatc cgcgtggccg aaaagttttt
    38221 aacagaagcc ccgctttctg cggccttctg caaaataacc ccgcgtgcat gccccatcag
    38281 aatcgccgtc tggggatgct tgtagtgtgc atacagctgt tcgacggcga cgatctgcgg
    38341 ccgatgggct tgcagaatct gcccgatatc cgccgcaatc tggttgagcc gcttttccag
    38401 ctcggctcgg ccgtcggtac gaaatacgcc ggcttccacc agccgggtat ccagccgttt
    38461 ccgctccaca acagcatagc cgcatacctg caatcccgga tcaatcccga gaatcttcat
    38521 tgccgtctcc agactgtccc tgcgggggta tcttccagca caatgccgaa ttcggccagt
    38581 ttgttccgta tcgtatccgc agccgcaaaa tcttttttct tccgggctgc ctgccgctgc
    38641 tcaatcatca atccaattaa atggtccagt agctgctcat caccgccgcc ggcggacggg
    38701 tagtcttctt tgacgatacc cagtacatcg ccgccaagtc tgcgaaactg ctcatccaca
    38761 gcccgcagcg tctcctgcgt cggctttccg gccagcgttt gatgcgccag cttgaccatc
    38821 tcaaatataa ccgacagcgc cacagcagta ttcagatcgt cattcattgc cgcttcaaat
    38881 ttctgccgca tcgccgcaag ccggccggct gcatcggcat caacctgtcc ggcaggagcc
    38941 gtccgaatcg ccttgcgaac cgccagcacc gcctctgtca tgcggtcata gccgctctgg
    39001 gcagccgtca gggccgcatc ggaaaaatct attgggctgc ggtagtggct ttgcagaatg
    39061 agctgccgaa ccgccagcgg actgtattta cggctcagcc gctcgtgcct ttcgaccggg
    39121 cagacatcaa aaatctgctt gagggtgata aagttgccca agcttttgcc cattttctgg
    39181 ccgtccaccg tgaccatatt gtgatgaatc cagtagcgga caaacggcac gccgttggcg
    39241 gcttcggact gggcgatttc gcattcgtgg tgtggaaact ggttttcgat gccgccgccg
    39301 tggatatcga tggttttgcc cagatacttc atgctcatca ccgagcattc gaggtgccag
    39361 ccgggatagc ccctgcccca cgggctgttc cactgcataa tatgagacgg ctcggccttt
    39421 ttccaaaggg caaaatctgc cggatgccgc ttctgggcat taatatccac ccgggcgcca
    39481 gccagcatct cgtccacatt ccgtccggac agcctgccgt aagcgggaaa cttgctgaca
    39541 tcaaaataga ccgatccgtc ggcctcatac gcatagccct tgtccagcag gatttggacc
    39601 agttcaatct gttcggggat atggccggtt gcccgcggac tgatgtccgg ccggagacaa
    39661 ttcagcgcat ccatatcctc aaagtagctg cgggtataat actcggcaat ggccatcggg
    39721 tgcttcttct cacgctgggc ctgcttggaa atcttgtctt caccggcatc ggcatcatcg
    39781 gtcagatgtc ccacatcggt aatattctgc acatacgtta ccgcataccc cagatagcgc
    39841 agataccgca gcagcacatc aaagctgaca tagcttttgc cgtgtcccag atgcgcatgg
    39901 ccgtaaaccg tcggtccgca gacatacatc cccacgcggc cttcatggag cggcctgaat
    39961 tcttccgttg ttcgggtcag tgtattatac agccgtacag tcataatctt ctcttgattc
    40021 tgcgtcgcag cattataacg gcagtagcgg caatggcctc tccccgccct actgcatcca
    40081 gtccctcgtt tgttttggcc ttgatattga cattggcaaa gtcggtttcc aaaaggcctg
    40141 caacctgacg cttgatttgc cctttaaacg gccccagccg cggaagctgg gcatggataa
    40201 ccacatccag attcaccact tcccactggt gttttttaac ctgttccctt gcccgcatga
    40261 ccagttcgct gctgtcggca tccttccact gcggatcggt atcaggaaac agcatgccga
    40321 tatcgcccag cccggcagcg cccagcatgg catcgataac cgcatgcagc acagcatcgc
    40381 cgtcgctgtg tcccagcagg cccagttcaa acggcacctg cactccgccc agcatcaggg
    40441 gacgctctgg caccagccgg tggatatccg tgccgatgcc ggtccggtat tcgtagtcca
    40501 gtccatccac gccctacagc acccccttgg tactgggaag ctggctgccg gtaattgccg
    40561 ctgccgcccc caaggcctga ccgagggcct tgaaaatcgc ctcggcgatg tggtggctgt
    40621 tggagccgta gggcacttcc gcatgcaggt tgaacttgcc ggcggtcgaa aaactccgca
    40681 gaaattcttc cacacactcg gtgtcaaact cgccgatttt tccgctggga aactgggcat
    40741 ggaaaaccaa atacggtctg cccgaaaggt ccactgccac aaccgccttg gcatcttcca
    40801 tcggcacaac gctgtgcccg tagcgggcga tgcccttctt gtcgcccaat gcttccaaaa
    40861 acgcctgccc aaggcaaatg ccgacatcct caatggtatg atgcgcatcg accgacaggt
    40921 cgcctttggc cttgacagtt aaatccatcc ggctgtgttt gctcaggtgc gtcagcatat
    40981 ggtcaaaaaa cccgatgccc gtgtcaatct cgtagcagcc ctgcccgtcc agatgcagct
    41041 ctgcccggat atccgtctca ttggtcttcc gccggatttc tgccttccgt tttgccatct
    41101 tgttttcctt aatccaacaa tctcttcaat gcggccagca ttgtatcatt ctgctcgggc
    41161 gtgccaacgg taatccgcag cttgtcttcc agacccggca gttcgaaata ccgaacaaaa
    41221 atattctgtt ctgtcaaggc attataaatc gccttcgcct ctttttgaac acaccgtgcc
    41281 aaaacaaaat tcgtctggct gtccggcaca tcaaagccca gtgctcgaag ccggcctatc
    41341 agccgtgttc gttccagctt gattttccgg atattgtccg caaaatacgc ctgatcgctg
    41401 acggccgccg ccgccgcggc gatagcggct gcattaaccg gataggaatc acgcactttc
    41461 atcagtccgg caaccagatt tttatgggca atcccgcagc cgaaccgcat tcccgccagc
    41521 gaatacccct tgctcatcga ccgcaggatg atgacattgt caaacttttt taccagccgc
    41581 aaggcattat cttcggcaaa atccgcatag gcctcatcta tcagcagcac gccgttcaga
    41641 tgcgctgcca gccgttcaag ggcctctgcc ggcagaaaat cgcaggtcgg agcattggga
    41701 ttgcatacaa tggtcagagc cgcattgatt tgggcaagtt ccgccagaga accgccggca
    41761 tcgcggcgga cttcaattgc cggacagccc tgcagctgtg ccagcaccgg atagagcgaa
    41821 taggtcggct gcgcatacgc caccggccgc tgggcatcgc agaacgcccg aacgcagata
    41881 ttcagcagat catctccgcc gttggtgcaa ataatgttgt ccggctcaac cgcccaaact
    41941 tcagctgcgg ccaaacgaaa tgcctgaccg agcgggtccg ggtagcggcg aagtttctcg
    42001 gcgtcgaggg tctgaaccgc ctgtatcaca cgcggagagg caggataggg attttcattg
    42061 gtattcagct tgaccacagc ggtcgatttg ggctgaaaac ccggcgtata cccggccatt
    42121 tgctcaattg tcggacggaa atatcccata ttttctccag ctgtcagttt cgggaattat
    42181 accccctttg gggccgcagc aaaagaagat tttgcacaac tggaagcggc ttatttgccg
    42241 cagtagtcaa tcagccggcc gatttcgctg cggagatttt tgcgatggac aatcatatcg
    42301 atgaatccgt gctccaagag gaattcagag gtctgaaaac cttcgggcag ctccacttta
    42361 atcgtctgct gaataacgcg gggtccggca aaaccgataa gggctttggg ttcggcaata
    42421 atgatgtcgc ccaaaagagc aaaactggca gccacgccgc cggtcgttgg atcagtcaac
    42481 accgaaatat acaatccccc tgcctgatca tagcgagcca gcgcagcact ggttttagcc
    42541 atttgctgaa gagcaacaag gccttcatgc attcgcgcgc cgccggaaca gcagacgacc
    42601 accagcggca gcgattcctc aatagccctg tccaccgcca tgcagaactt ctcgccgacc
    42661 accattccca tcgaaccgcc caaaaaactg aaattcatca ccgccagcat cacgccgcgt
    42721 ccccggatga aggccttgcc tgccagcatg gcctcatggg cgctcatctg ggccggatcg
    42781 atctgaatgc ggtctttgta gcttttatcg ccccatgtga attgcagcgg atcgctgctg
    42841 gccaaatcag agccgatttc ctcaaacgac ccttcatcgg ccagctgccg caggcgggta
    42901 accccgtcaa ttcgaaaatg atactgacat tccggacaga cgctgagatt ctcgtccact
    42961 gtcttgcgaa agagcatatt gcggcaggat ggacacgaca gccacagccc ttccggcatt
    43021 tcccggcggg gtttcagcga aaaaccattc cagcttgctt ttttcttttt aaccatttgc
    43081 atgcactatt gctgtaacgg aaaacggctg tcggccaagg ccttccgcaa ggcggctatg
    43141 gccgctgcac gatcagtcct gccgaaaatc gcatttccgg ccacaaatgt atcggcgccg
    43201 taatcgcggg ccgtacctat tgtagcggca tcgatgccgc cgtccacttc aatccggaca
    43261 ttcggccctg caatctgccg aacccgacgg catttctgag cggccttctc gataaaggac
    43321 tgaccgccga agcccggatt aaccgtcatt accagcacca tatcacacag cggagcaacc
    43381 ttttcaatcg tctccaccgg cgtcggcgga ttcagtgtta tgcccactgt aactcccata
    43441 tcgcgaagca tgttgacaaa atccgccgga ttgtcaaccg tttcaatatg aaaggttata
    43501 tggtccgcac ccgctttgac aaactgcggc gcataggttt gcggatcggt tatcatcaga
    43561 tgcgtatcca gcaccgcatc ggtgcattgg cgaatccatt tcacgaccgg aggaccaatg
    43621 gtgatattcg gcacaaaatg cccatccatt acatccagat gcaccatggt gatgccttcg
    43681 tgcatcactt gtgctatctc cgaagacaag ttggcaaaat ccgcactcag gatagacgga
    43741 acaatctgat gcgttcctgc tgctggcaat ctgcgcataa cgtttatact gtttcaaatg
    43801 tccgcagaag gcacagaaaa tccaatcccc aacggcggat tttctgtgcc cctgcagtgt
    43861 gggcttactt atcttcgagg atgtcgaggc acttaaactt cttgccctca aggaattcca
    43921 gactggctcc gccgccggtg gagatatgac tgaccttgtc agccagtccc atctgcataa
    43981 cggcgctggc gctgtcgccg ccgccgatga cgctgcgggc gccatgggcg gtagcatcgg
    44041 cgaccgcttt ggcgacggcc tttgtgccgg catcaaacgg gggcatctca aagacgccca
    44101 tcggaccgtt ccagacaatc gttttggctg ttttgatttt ttcagcaaac agcgtggcgg
    44161 ttttgggtcc gatatccaga ccttcccagc cgtccggaat gctgccttcc acaactttat
    44221 gttcggcatg ggctttgaac tcggcagcaa tgaccgtatc cacaggcagc agcatttcgc
    44281 agccgatctc tttggccttc tgaatcagcc ctttggcatt ctcgacgaaa tcattttcgc
    44341 acaagctttt gccgaccgtc tggcctttgg ccttgaagaa ggtataggcc attgctccgc
    44401 caatcagcag gcagttgacc ttgggaagaa ggttttcaat cactttgagc ttatcggaaa
    44461 cctttgcgcc gcccaaaatc gccacgaatg gccgctcggg tttttccacc gtttcgccga
    44521 ggaacttcag ctccttttca atcaggaaac cggacacccg cggcttgtct ttcatcatgc
    44581 agggcacggt gtacatcgac gcattgtcgc ggtgtgctgt gccgaaagca tcatcgacat
    44641 acacatccgc caaatccgcc agctgcttgg caaaggcgtc tttggcagcg cggagcgccg
    44701 gatcttcctt ggcggcctta tccttgatga cttcttccgg atgaaaccgg acattttcga
    44761 gtaccagcac atcgccgggc ttcagagcgg cggctttggc tttcacttcc ggccctacgc
    44821 aatcgccgac aaactgcaca ggcctgccca gcagctcgcc cagccgcttg gcaaccggcg
    44881 ccattgaata tttcgggtcc ggagcccctt caggccggcc cagatggctc atcagaataa
    44941 gcgccccccc gccggccagc acatgctgaa tcgtcggaag cgcctgcaca atacggttat
    45001 cattggtgat attctgattt ttgtccagcg gcacgttgaa atccacgcgc atcaggactt
    45061 tcttgccttt gacattgata tctgcaacag ttttttttgc catagtggtt ttccttatcc
    45121 agcatccggg ataaaa
//
LOCUS       DAOTBO010000015        42548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_743_length_42548_cov_5.414586, whole genome shotgun sequence.
ACCESSION   DAOTBO010000015 DAOTBO010000000
VERSION     DAOTBO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 42548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42548
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_743_length_42548_cov_5.414586"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLE88_03355"
     CDS             complement(<1..191)
                     /locus_tag="PLE88_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOM75537.1"
                     /translation="MAHCPDTDERMNIVIAGHVDHGKSTVIGRLLADTDSLPKGKLEQ
                     VRQTCRRSSKPFEYAFLLDA"
     gene            complement(224..1105)
                     /gene="cysD"
                     /locus_tag="PLE88_03360"
     CDS             complement(224..1105)
                     /gene="cysD"
                     /locus_tag="PLE88_03360"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOM75538.1"
                     /translation="MDHLEQLENLSIHILREAYASFKNLCMLWSIGKDSTVLLWLARK
                     AFLGHVPFPLVHIDTHYKIPEMIAYRDRLTRQWNLDMIYGQNTEALEAKKTFPDGNAD
                     RLTCCKLLKTEALKHTLSGQWPRYRFNHAAGHYEADANTEPFTGVIVGLRADEEGSRS
                     KERYFSPRDRQSEWDIADQPPEFWNQFKTDFAPGTHVRIHPLLDWTELNVWEYIEREK
                     IPTVSLYYNQGDGKRYRSLGCWPCTFPIDSQARNPQEIIDELRSGQLANIAERSGRAQ
                     DAEDGGGLETLRREGYM"
     gene            complement(1109..1645)
                     /gene="cysC"
                     /locus_tag="PLE88_03365"
     CDS             complement(1109..1645)
                     /gene="cysC"
                     /locus_tag="PLE88_03365"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOM75539.1"
                     /translation="MSEKTGKKGFVLWLTGLSGAGKSTLAERIYNHLEQMGKPVERLD
                     GDVLRGVFPNTGFSKEARDEHIKRVGFMASRLEHHGVIVIASFISPYRAARDFVRSLC
                     RNFIEVYVKASLEECRRRDVKGLYQKALAGQIREFTGIDDPYEEPLHPEIVVDTEQQT
                     VEQSVRQIMDYLKPYLQE"
     gene            1957..2346
                     /locus_tag="PLE88_03370"
     CDS             1957..2346
                     /locus_tag="PLE88_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOM75540.1"
                     /translation="MKLTEAEWQIMNALWQDWPATARQIAERLPKDVNWAYTTIKTML
                     KRLSDKKAVKEIKNGNVGVYEPILTRQNARRNALKLLVNQAFDGAFGPLLHFLLEDQK
                     LSDKQKEELLRVLQQGEQKGAHNDPVF"
     gene            2330..2533
                     /locus_tag="PLE88_03375"
     CDS             2330..2533
                     /locus_tag="PLE88_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75541.1"
                     /translation="MIQFFNTISDKWLAFQWAMLWQTAVLIAIIWMLDVLIRKWAWPQ
                     VRYALWLLVLVKLLLLPTLTRRR"
     gene            complement(2598..2840)
                     /locus_tag="PLE88_03380"
     CDS             complement(2598..2840)
                     /locus_tag="PLE88_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75542.1"
                     /translation="MSIRYDFHESAAGCSTLVSTEEVTAAEISAARTSLPRSGSAVLL
                     RLTPAFSAACYLLDAIVVQFVSGKTDILSPAGSGAA"
     gene            2758..3675
                     /locus_tag="PLE88_03385"
     CDS             2758..3675
                     /locus_tag="PLE88_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOM75543.1"
                     /translation="MSAAVTSSVETSVEQPAALSWKSYLMLTWMLGTAILTVWLVVRL
                     LGFGREYLKDDGQYQLPERFYAQLHAAGERLKLRRLPRVILANKVSCPAVFGIFCPVL
                     LMPAEQFRTMSNPDTEHILLHELAHIKRGDLIVPAIYMVLQIIYWFNPLLWMIRKTLQ
                     NLRELCCDAAVARLLRENTVHYRRTLLETARQLLAEPVDPGLGLLGLFENSNWLVTRL
                     QRLEKKTWKNCPLRIATVITLVSVMTVCVLPLGNRTKEQQLAFYMGYLRIDSSIGDIS
                     RAVLSMEHDYDFSQPYAGLCGQDSAAGNE"
     gene            complement(3728..5668)
                     /locus_tag="PLE88_03390"
     CDS             complement(3728..5668)
                     /locus_tag="PLE88_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOM75544.1"
                     /translation="MLASVVSAADNNSSQEDFFNMPLESLMDIEVYSASKHTQPASQA
                     PSSVTVITQEEIKRYGYRNITDILNSVPGFYETYDRNYSYMGVRGFGRPGDFNSRILV
                     LVDGQRLNDNIGNTALLGNESLIDADLIDRVEIVRGPGSALYGSNALFAVVSIFTKSG
                     SQYNGGQIAGRFGSRRHTGGRFTYGSKTDNGIEFLASGTYADWEGDRLYYREFDDPST
                     NFGRTRNDDENLHNMLLKGSYRDFTFFAAGSRRQKGIPTASYETVFGAEDTRTWDDYT
                     LIGLSYNHQIDEDFSILWRLSYHHYNYRGHYVYDDGGLYENKDWWKGRWITGEVQITK
                     RFDDHHTLIVGAESQTNIRQDQKNWDFDVYLNDRRHSKNWGIYIQDEIRLTDRLTVNA
                     GIRRDYYDQTGAQLNPRAAVIYQLTDTATVKLMAGRAFRTPSVYELYYQDNGNTAKAN
                     PNLQPETIKTYEAVFENRFSEKWRGTFSAFHYQVNDLIDQTIDPDDGLSQFRNLSQVT
                     ADGYEAALIGKWANGLQTRSSYASVRTLDKANRSSLANSPAHLVNFNLIYPLLENKLF
                     AGLDIKYTSKRKTLSGGHTDDAVITNLTLTYDNILKNLDFQIGLYNLLDVNYEHPGFT
                     EHLQDKIEQDGRTFGVKLTYRF"
     gene            complement(5855..6538)
                     /gene="nfi"
                     /locus_tag="PLE88_03395"
     CDS             complement(5855..6538)
                     /gene="nfi"
                     /locus_tag="PLE88_03395"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOM75545.1"
                     /translation="MQPISNLHQWDLSPAQAKQLQKTLAGQVRLQPMRKQPRCVAGLD
                     CALNKAQTAIYAAAAVFSFPDLKPLETAVARLPLVFPYVPGLLSFREIPVYLEVIMKL
                     QNPVDLFLIDGQGIAHPRRLGLASHLGLFLNTPTIGCAKSRLIGTFDTPPLLKGHFSW
                     LYDGADKIGAVLRTRSGTKPLFVSPGHLCSFQDAVDYTLACCTKYRLPEPARLAHQAA
                     SNAKTSAAF"
     gene            complement(6633..>7226)
                     /locus_tag="PLE88_03400"
     CDS             complement(6633..>7226)
                     /locus_tag="PLE88_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75546.1"
                     /translation="KTQRHYIAIGYGLADWWEVYAQLGLADVKTKGNAVYDDGDSWKG
                     GINFDNDFAWGLGTRFTFAEQDKVKWGLSAQMNWLDTSWDEKGSEDGEITYLWKDTID
                     IESFDLLVALGPTVDMGCWKLYGGPFYYYLNGDYDYKSVDNLGWVYKESADLEEDSSF
                     GGFIGAIFPIGNNMDWTTEFSATGDGWAIGTGIGWKF"
     assembly_gap    7227..7326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7327..7518)
                     /locus_tag="PLE88_03405"
     CDS             complement(<7327..7518)
                     /locus_tag="PLE88_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75547.1"
                     /translation="MNKGMIVLAVLVASMSVGALAVGFMGPPTAELKQGQWNLGYNYT
                     YSDMDLEKTSLKWAWFDVGV"
     gene            7777..10629
                     /gene="uvrA"
                     /locus_tag="PLE88_03410"
     CDS             7777..10629
                     /gene="uvrA"
                     /locus_tag="PLE88_03410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOM75548.1"
                     /translation="MSEAKYIRITGACEHNLKNISIDIPRDKLVVVTGLSGSGKSSLA
                     FDTIYAEGQRKYVESLSAYARQFLEQMQKPQVEHIEGLPPTIAIEQRSASSNPRSTVA
                     TTTEIYDYCRLLFARAGTPHCWICGKVISSQHATQIVDSILQWPAETRIQICAPLISG
                     QKGEHRDVFVQIQREGFVRVRVDNQIYDAKAVPLLDKNKKHDIAAVVDRLVLKEGIRI
                     RLADSIETALKIGDGVVLVMKQEPSAQKTEGGNGGWTDVVYSEKYACVDHPQASLAEL
                     SPRLFSFNSPYGACPSCDGLGTILEFDPDLIVPDKSVSLENGAIEAWRKGGKRMNIYY
                     NRLVKRFCRNFNIDKAAPFSEIPADIRKILMYGTTDADEAAYGVSFEGVIPNLVHRWK
                     HTTSEFVKERLHGYLSEQPCKQCGGTRLRPEAAAVTIGDKNICAITRMSIDQAYSYFK
                     SLRLDEEKTQIAAQPLKEILARLKFMKDVGLGYLTLDRTSRTLSGGEAQRIRLATQVG
                     SGLVGCCYVLDEPTIGLHRRDNDRLLGILKKLRDIGNTVLVVEHDDDVIRSADHIIDI
                     GPAAGRHGGELVAAGTLDAICRCERSITGQYLSGAKRIELPAQRRKVLMSNCIEVRGA
                     EEHNLKNITVKFPLGVLTCVTGVSGSGKSTLVTEILLKALKRRLYGSPEKPGRHKSIS
                     GINRIDKVIEIDQSPIGRTPRSNPATYTGVFDLIRQLFAMTREAKIRGYKPGRFSFNV
                     KGGRCEHCQGQGTKKIEMHFLPDVFVTCQECKGTRYNPETLQITYKGKNIAEVLDMRI
                     DDARQFFANFPKIVRLIKALCDVGLGYMTLGQSSTTLSGGEAQRVKLAAELGKPGTGH
                     TLYILDEPTTGLHFADIHNLMNVLRRLTDMGNSVIVIEHNLDVIKMADYIIDLGPEGG
                     EEGGRVIAAGTPEKIIQNDHSYTARYLKEKLAAERG"
     gene            10673..12007
                     /locus_tag="PLE88_03415"
     CDS             10673..12007
                     /locus_tag="PLE88_03415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM75549.1"
                     /translation="MSHQQKLPFIGLYQRCWDSIRTTFRSLRYRNYRLFFIGQGLSLV
                     GTWMQQVAVSWLVYRLSHSAVALGAVAFAGQAPVFLLAPVGGITADRCSKHRILLLTQ
                     MLSMVQAAVLSVLVLSGTVQVWQIGLLSLMLGTINAFDMPTRHAFVIEMIEDSGDLGN
                     AIALNSSMFNGARLAGPALAGVIISIFGESLCFVLNAVSYLAVILSLLKMQIGRFEPP
                     SKPVHPVAQLKAGFAYAYRFTPVRDVIGFIAAMSLVGMPYAVLLPVLAKTVLDGGPQT
                     YGLLIGSAGAGALFGALYMAWRKTVRGLGRILVFGGSLFGGSLLVLSAAESAGFSMAL
                     LLLCGFGMMVLMAGCNTILQTMVDDSKRGIVMSIYMMALIGMAPLGSLAAGFMAHRLG
                     LHLTLLMGGLLCLLASGLFALRLPRLRGIVRPVYVEKGIITETAGRVQDAGE"
     gene            12336..13067
                     /locus_tag="PLE88_03420"
     CDS             12336..13067
                     /locus_tag="PLE88_03420"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOM75550.1"
                     /translation="MKSNCLQIEQVQMSFSRFLWKSTAAACWFSVLLTVIVGSVQAVV
                     QPPAAITADLNNKGAQSIEYKVKAAFIYNFTKFTVWPPEKHQASSEQQNGQASQTKPL
                     FIGILGKNPFEDAFVPILNKDVGRRMELVEIGSFAEFQRSYGRRSDALDAYRKANEQT
                     LAKCDVLFICDSEKSFVSELITLTAGRSILTVSDMANFISNGGMISFVKEDNKVRFEV
                     NLKAAQKENLKIGSQLLGLARQVHK"
     gene            13124..15145
                     /locus_tag="PLE88_03425"
     CDS             13124..15145
                     /locus_tag="PLE88_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75551.1"
                     /translation="MKKLVIHTIRKKLIGIIMTICVVCLVMSGAAFSLWSYISFRNEM
                     ISSMTVQAEMTAGHCSAALAFDDAAHAEKILASFAAKPSIVSCCIYNAQGKHFAAYIR
                     KDFADHHTAVTLQNEGHRIDKGYLQIFMPIVLDGEKIGTVVLVSNLEPLKANFRNNGF
                     IIAAVTIAAALGGYLLARKFQQIVSKPILELAALARAVREKRDYTQRAASISQDEIGI
                     LTEAFNQMLDEIQKEMEDRIKAQNELIQHRDQLEEMVAERTSELKSANYNLAMAVEKA
                     NLMARQAQEANRVKSEFLANMSHEIRTPMNAIIGFSELLIEEPLDEQQRSFVQTILNS
                     GQNLLRIINDILDFSKIEAGKLQTEIIECDLQGFLGDIESLFSPAAREKGVDFKVLCC
                     DELPVKIYTDPIRLRQCLLNLCSNALKFTAQGHVYVNVSTERDGKEDFIRFDVEDTGI
                     GIPKDKLNAIFEAFTQADNSTTRKFGGTGLGLTITRQLTHLLGGDITVRSEEGRGSVF
                     TMRLPIGTCSKKPAIEPYPVLNEMAALSEAAAQPPAPDPAGRILVAEDAVANQKLITI
                     LLERKGHKVTLVENGQQAIEAVQKNSFDLIFMDMLMPVMSGYEAVRQLRAAGCTLPII
                     ALTANAMSDDEKKCLQCGCDEYISKPINRQKLDALLTKYLRQPAVESRS"
     gene            15420..15764
                     /locus_tag="PLE88_03430"
     CDS             15420..15764
                     /locus_tag="PLE88_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM75552.1"
                     /translation="MKFERELLKGVAPVVVLEILSRGKMYGYELSEALEQRSRGILTL
                     GRGTLYPLLYNLEAKGLIAAEWEESKSARKRRYYSITSSGKAELAAKKEQWMALQQGV
                     NLVFGLGSLSPA"
     gene            15822..17468
                     /locus_tag="PLE88_03435"
     CDS             15822..17468
                     /locus_tag="PLE88_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75553.1"
                     /translation="MEKKCLSDLPRVAVEYIDSVIKAMKYRRNVRIEVRQELIDHFAD
                     ALADCKTNDEKQKLAEELIAEFGDVKLLGTLLRRAKKRCRPLWRTMVARAFQLVGVLF
                     VLLVLYIGWFFSGKPVITTNYLEVLNRQVRPVADDSQNAWPFYKQAVEKYVQYEANEE
                     REKFDFRNPSTLSTEERGFVQKSISGNQQSLDLIRQGNQRPYYWPVYDVQEGQNPELI
                     AISIPHLKEYRRLAELLCWQGLLSAEAGRLDKAFQEVLESYSFGKHLRGQHTMLIEQL
                     VAIALEAMSTKTLRIVLSEYGEHIDAAILEAARKRFEAEIADEDFTADFDGEKLFIYD
                     EIQRSFTQSRIGKSHLYIKRLEQNVPALAFGAQGKLVKHWFRILFTHPGKEETLKMSE
                     EFYSAIKANAGKTPAEIKLLGTETKADKIEKEIFNDNIFLGLLMPAFNAVISRTYRNR
                     IDSEATLTVLAILEYQKQKGVLPENLNVLVEDGLLKEIPIDPFSDKPLVYKKTDGGFT
                     LYSVGLNFMDDGGTRSKDSHDRPALWGEGGDAVFWPVTDS"
     gene            complement(17489..17755)
                     /locus_tag="PLE88_03440"
     CDS             complement(17489..17755)
                     /locus_tag="PLE88_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75554.1"
                     /translation="MPLSKHIILGVHITDRIRHVEAVQHLLTEYGCSIRTRLGLHETD
                     KGFCSPNGLLILEMTDDDAKTTELMGKLNAIEGVEVKKMIFDHA"
     gene            complement(17772..18860)
                     /locus_tag="PLE88_03445"
     CDS             complement(17772..18860)
                     /locus_tag="PLE88_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOM75555.1"
                     /translation="MNPAVLEIVRRLLHQPTAPYREAAVRRYIADFCKARSIQIHQDR
                     VGNLTAVYGTSYPDSKLAFAAHMDHPGFIIETDAHRRRTTALFYGGLKDAYFRSAGIC
                     IFCQSQIVKARIIKMQSDKRFRRRRIWLQVSGDVRQGAVGMWDLPACRIRGSRLYSRA
                     CDDLIGCASILALLDLLAQRRIRKKVTALFTVAEEAGLHGAKHLCCSGRLHSGLRIIS
                     VETSAALPAAKMEDGVIVRVGDRASLFSPGLTAFLLEAARRIQSKDNTFKYQRKLMDA
                     GTCEATVYSRFGYAAAGICIPLANYHNCNARTGRIAPECVSLGDLENMVKLFAGVVKY
                     CRKADEFLNAAPPAYQQSSGKLGEFFYT"
     gene            complement(18841..19296)
                     /locus_tag="PLE88_03450"
     CDS             complement(18841..19296)
                     /locus_tag="PLE88_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOM75556.1"
                     /translation="MKHTQKAVQLFGSGLNCAQAVLAAFAEELGMDTPTALKVASGFG
                     GGIGRTGQVCGALTGAVMVLGLKYGCGQAMDKTAKYELYRKVQKLTEEFKLRTGSLYC
                     RDLLGFDFLTPQGQMMAAQPGAFEQCDGFVHTAAELAEEMLQADESCSA"
     gene            complement(19310..21088)
                     /locus_tag="PLE88_03455"
     CDS             complement(19310..21088)
                     /locus_tag="PLE88_03455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOM75557.1"
                     /translation="MIKVNNQKADSSASKNNDISLETLPPYQPRRFVPAGLELTNRDS
                     VAALYRQLADRPIDNTDQLEQWLADRSELEAAVAQCQSILYIRMTCQTDDPQRAAAYR
                     TFMDTVVPAVKPLSDKLDRKWLLLTEQLQYQPPRYAVYSRTVRSDVQLYRDANVPLQT
                     QDALLSQQYQTLTGAMTVPFEGRIWPIVQMRKFLLETDRPLRQRAWMAASRRYLQDKD
                     QLDDIFDKMISVRSRIAENAGFSNYRDYKFREYHRFDYTPQDCRQFHAAVRQHLVPLL
                     KEMLGYRAKQMGLDKLRPWDLNTDPLGRAPLRPFETTQQFAAGVYQMFAQLDRQFGEG
                     FRMMMDSGLLDLESRPGKAPGGYQSTLYEARKPFIFMNAIGTNDDLCVLMHEGGHAFH
                     TLACADEPLWAYRHAPMEFCEVASMSMELLAGAHLSVCYDQAQRRRWWREQLETIVRT
                     LVSVAVNDAFQHWLYEHPAHTPHQRNAQWLALNQQYEDGIVDWSGLEDCRASMWQRIL
                     HFYQVPFYYIEYGIAQLGALGIWLASLKNRQQAIAQYKTALALGGSRPLTELFAAAGI
                     PFDFSADTIKPLAQAVRSQWMEYCIT"
     gene            complement(21187..21573)
                     /locus_tag="PLE88_03460"
     CDS             complement(21187..21573)
                     /locus_tag="PLE88_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75558.1"
                     /translation="METETSNANYLETISILHYVLAVLIYFKGLIAPFIIGIAAVALS
                     HSPHDTNLFVLFLTFFAALLLILPLTCIFGTLVLLAGRYISTRKYLVYCQIIAGLECL
                     CVPLGTILGIFTLIQLTKPTVKATFK"
     gene            21771..22088
                     /locus_tag="PLE88_03465"
     CDS             21771..22088
                     /locus_tag="PLE88_03465"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM75559.1"
                     /translation="MQYLYEKQAAIAKAIAHPVRVAVLDFLRTGEQCVCDIAAAVGSE
                     RTNLSKHLSVMVAAGVLESRKDGLKVMYRIKTPCVLRFLECLTGCLREQAAEQRKFLE
                     AIH"
     gene            22121..23452
                     /locus_tag="PLE88_03470"
     CDS             22121..23452
                     /locus_tag="PLE88_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOM75560.1"
                     /translation="MNLKAEWKKLAWIISVFLLCFYLPVGVGRFDNAVSEALSLTKWY
                     AREHVLLCLVPAFFIAGAIGVFVSQGSVMKYLGPKANKVLAYGVASVSGTILAVCSCT
                     VLPLFAGIYRMGAGLGPAVAFLYSGPAINILAIIMTARILGMEMGIARAVGAILFSIV
                     IGVLMHLIYRKEEIAKAEAAMAVPQAASSRPLWKNAVFFAAMVLILVAANWARSGDVR
                     AFFLCCPGGLQTYAVEGKLLEQTDTTVTVVDNTGTKHVIPVDQFKGVEPIKKNPVYEW
                     ISQARWGIVAAAGVLLAAMCIFWFTKPELIEWVDASWTFAKQILPLLLIGVLAAGFFL
                     GRPGKEGIIPNIWVEKALGGNGLGANLFAAVAGAFMYFATLTEVPILQGLMGSGMGKG
                     PALALLLAGPALSLPNMLVIRSIMGTQKTVVYVLLVIVMAAISGMLFGAFF"
     gene            23462..23968
                     /gene="tsaA"
                     /locus_tag="PLE88_03475"
     CDS             23462..23968
                     /gene="tsaA"
                     /locus_tag="PLE88_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOM75561.1"
                     /translation="MVDKADEFTVKAIGIIRTPFKHPQEAPIQPAFAKGAEGVVDIFE
                     PYEAALKDLDGFERIWLIYYCHRAAEWKPLSKPYLDTVERGLFATRAPARPNPIGISS
                     VRLVRIEGRRLYVRDIDALDETPLLDIKPYSGRFDVFETKYNGWMDAVDTSNPKADGR
                     FWKPQTSE"
     gene            23981..24211
                     /locus_tag="PLE88_03480"
     CDS             23981..24211
                     /locus_tag="PLE88_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOM75562.1"
                     /translation="MKKIQILGTGCPKCKKLSENAEAAAKEMGIEYTLEKITQINDMM
                     KMGVMVTPALAVDGVVKTAGKVLTVEEIKKLL"
     gene            24240..24614
                     /locus_tag="PLE88_03485"
     CDS             24240..24614
                     /locus_tag="PLE88_03485"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOM75563.1"
                     /translation="MADRESCLCSGGPKLIFACSGAADVGAIADQAARKMSKDGIGKM
                     FCLAGIGGRIDGIMKTTEAASMILAIDGCPLNCVKACLEKAGFTAFKHLQLADLGLEK
                     GKSPVTEEAVSRVADKAKLMLA"
     gene            24777..26108
                     /gene="merB"
                     /locus_tag="PLE88_03490"
     CDS             24777..26108
                     /gene="merB"
                     /locus_tag="PLE88_03490"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HOM75564.1"
                     /translation="MNKMHKIMIVMALAVLVGVVIVMKQQEPQSPALQNTSTQQAQTA
                     VSELPVENKAASPMPAAKPGTLPMLLDLGADKCVPCKMMAPILEALKKEYEGKLHVVF
                     IDVWDNPNEASRYGIRLIPTQIFYDAGGRELFRHEGFISKEDILTKWKELGVNLADAG
                     GMPPAFERLVPARADTRPKEQICYMCDGDISPQTLVTVKTDKGDVRLCGLHCYFIMYS
                     CLMEDKTDFENKVTVSDFESGRPVPLTEAVFLAGTDAKGRPMLRAYANADAANQARQT
                     DGGNVIGLQALRQNELSCRCGFCDRACYPQDAAEVIVEGGLRTWGCCSHCALGVAART
                     GKDIEIHEKDRLTGEPIIVKTLNGSILSLDPPTAVAWFGQRQKPDGSWTSAGCFHQGF
                     FVNPDNLIKWVEQNPYETGRLISIHQALADKMKLSPEQIQKACKIGECTPK"
     gene            26146..26841
                     /locus_tag="PLE88_03495"
     CDS             26146..26841
                     /locus_tag="PLE88_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOM75565.1"
                     /translation="MQDLFTQLTRAVEGAWFIAVPASLIWGILSILLSPCHLASIPLI
                     VGFIDGQGRISAKRAFWISTVFAVGILITIGAIGAITAALGRMMGDVGRYGNYFVALL
                     FFAVGLHLLDVIPMPGSGPGQIGIKRKGMLAAFILGLVFGIALGPCTFAYMAPMLGVT
                     FKLAMTNFSYGVVLLLAYGIGHCSVIVFAGTFTEVVQRYMNWNEKSKGADNLKKICGL
                     LVLLGGVWLIYTA"
     regulatory      26904..26967
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            27052..27429
                     /gene="crcB"
                     /locus_tag="PLE88_03500"
     CDS             27052..27429
                     /gene="crcB"
                     /locus_tag="PLE88_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOM75566.1"
                     /translation="MLQKLLWLGLAGAAGTLTRYWLSGLVQKNVSVDFPIGTMTVNIV
                     GCLLFGLLWAFIESRLSISGQMRMIIFLGFFGAFTTFSSFAFETSQLLDESQWLWAAG
                     NILLQNILCLAGMITGLTVGRWI"
     gene            27450..27803
                     /locus_tag="PLE88_03505"
     CDS             27450..27803
                     /locus_tag="PLE88_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOM75567.1"
                     /translation="MQLPSEAQLLRIFIGEADRFGGKPLYEAIVHLARQKGMAGATVV
                     RGLMGFGAHSRMHTAKILRLSEDLPIVIEIVDKPERIKDLLAELDGMIKEGLVTLEKV
                     TVFAYRCENGNRKFI"
     gene            28001..29230
                     /locus_tag="PLE88_03510"
     CDS             28001..29230
                     /locus_tag="PLE88_03510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM75568.1"
                     /translation="MAFSIEIILSPIEQKGQFFMNNHAHRRAMGFVLLLGVVSLFADM
                     TYEAARSLSGPYLAILGASGAIVGFTAGAGELLGYGLRFFSGLLSDKTQRYWLMTIAG
                     YAVNLLAVPLLALAGHWGWAVGLIMAERFGKALRTPARDTMLSCATSVVGRGWGFAVH
                     EAMDQIGAMAGPLIVMIVLAVNSSYRFAFAVLAIPAVLALSVLIAARVIFPHPHKLEE
                     EIPQQGDGKFPAVLWLYLAAVGFVAAGFADYPLIAFHIKSHALLADKWIPLLYACAMG
                     IDALAALGFGRWYDKNGVLALAGAVIISSIFALFAFSFRPAAVIAGILLWGIGMGAQE
                     SIIRAAVADMVPRHRRGTGFGLFNSCFGIAWFAGSFLMGILYDVSLPALIGFSIAAQL
                     ASLPLLLAVQKRLSPSK"
     gene            complement(29232..30416)
                     /gene="arsB"
                     /locus_tag="PLE88_03515"
     CDS             complement(29232..30416)
                     /gene="arsB"
                     /locus_tag="PLE88_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOM75569.1"
                     /translation="MSNPQAAKGLNIFEKYLTVWIILCILGGIVMGKLAPGLAASLDG
                     MAIYVNGAPVVSIPIAACLFLMMYPIMVKIDFAEVLKAGKSIKPVGLTLFINWAIKPF
                     TMYAIALFFLGTLFYQFIGPNAVDWVKMPLGLDLPVGAEYGVGTIVLKNGVKMLQVPL
                     WRSYLAGCILLGIAPCTAMVLVWGYLAKGNDGHTLVMVAINSLTMLLLYGPLGGFLLG
                     VGKLPVPWQALLLSIAIYVALPLAAGFISRKWILAHKGADWFEQKFLHILTPITILAL
                     LLTLVLLFSFKGQTILNNPLTILWIAIPLFIQTCLIFAIGYGLSKMLNLSYPDAAPSA
                     MIGASNHFEVAIATATMLFGLQSGAALATVVGVLIEVPVMLMLVKICLRTQNWFSRSV
                     RP"
     gene            complement(30419..31465)
                     /gene="arsM"
                     /locus_tag="PLE88_03520"
     CDS             complement(30419..31465)
                     /gene="arsM"
                     /locus_tag="PLE88_03520"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOM75570.1"
                     /translation="MTGKGNNKIRDAVRLGYADIARGQSGCCCGSAEPNKLAEGIGYR
                     REELDIVPQGANMGLSCGNPTAIADLKPGQVVLDLGSGGGLDVFIAASKVGPTGYVIG
                     VDMTAEMVSKARAGIEAFAEKTGLSNVEFRLGEIEHLPAADASVDVVISNCVVNLSPD
                     KQQVWKEIARVLKPGGKACISDLALTNPLPQKVQESIAALIGCVGGAVLIERTQAMIA
                     AAGLVHPQVAVKPSNMDRMASCNDSLYQSIRQHLPADKKLSDYIVSADFIAYKDHSAA
                     VSSATKELIALGASVACNCHPCVIFHTNKAQQMGLGHTEIQQAVHIGKMVRTGAARQM
                     DELLEKEIPHHNKE"
     gene            complement(31480..31920)
                     /locus_tag="PLE88_03525"
     CDS             complement(31480..31920)
                     /locus_tag="PLE88_03525"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOM75571.1"
                     /translation="MKQRVLFFCKHNSCRSQMAEAYLKKLAPDQFEAISAGLYPQMIH
                     PLVYLVMQEDGIDIAGQTAKSIDLYLGKELLDWVIIVCQEGEAECPQLMPLALHTERW
                     PLADPAKKTGPKEEVVDAFRQTRDAVKQRVQNWLTMHNYPDKTD"
     gene            complement(31917..32705)
                     /locus_tag="PLE88_03530"
     CDS             complement(31917..32705)
                     /locus_tag="PLE88_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM75572.1"
                     /translation="MKQEAAILKVLADPIRLRLMTLLAQSGQLCVCQLVDALNEPQFK
                     ISRHLGELRKARLVTAQRQGTWMHYRLSPAATMFEQSLHNFFRDYLKDHPQAQQDLKR
                     LGESNCRVPQKGKNKLKILFLCTGNSCRSQMAEGWARHLKGDILEPYSAGIETHGLNP
                     CAVKVMAEAGVDISQHRSKHLNELAGMDFDYVITVCDNARESCPLFSGKAKSIHINFE
                     DPPRLAKDAKTEEDALNCYRRIRDEIRQFIETLPEALTDQRSGL"
     gene            complement(32790..33248)
                     /gene="dtd"
                     /locus_tag="PLE88_03535"
     CDS             complement(32790..33248)
                     /gene="dtd"
                     /locus_tag="PLE88_03535"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOM75573.1"
                     /translation="MRAVIQRVKKASVSAEGQITGRIEQGLLVFLGIGRDDSRADIDF
                     IADKITNLRIFEDKDGKMNLSVRDIGGGILLVSQFTLYGDCRRGRRPDFSAAGTPQVA
                     KILYQQTIDVLRRSQLAVETGVFAAYMIVDAVNDGPVTLIIDSKKDIKKL"
     gene            complement(33252..33737)
                     /locus_tag="PLE88_03540"
     CDS             complement(33252..33737)
                     /locus_tag="PLE88_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOM75574.1"
                     /translation="MQNQIQTTYRRILFCTDLSENADFAFQFAVDAAMRRPGSELYLL
                     HVIPETEAQFWKTYIYEVENVDNKAKRDLDERIAEAYVSKLPEGLKLHIEYRIGKDWQ
                     EIVAFAKEMEIDLIVMGRQGRGALQKTLFGNVNEKVVRKADCAVLVVPLSYQKRLQEK
                     G"
     gene            complement(33767..35068)
                     /locus_tag="PLE88_03545"
     CDS             complement(33767..35068)
                     /locus_tag="PLE88_03545"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOM75575.1"
                     /translation="MTPLQYGIIGCAVLFVLLAASMPVAFTMAIVGFAGFAAIVTPKA
                     ATAMMTTELYETFTQYSLTVIPMFVFMGQIAFHAGISRRLFDTAYHWLGHLPGGLAIS
                     TVGACTAFGAICGSGPATAATMAAVALPEMKRYKYHPELSCGAVAAGGSLGMLIPPSV
                     VFIVYAILTEQSIGKLFIAGIFPGLLMALLFCLTIYIKCKINPVLGPCGPRLGWGAKI
                     VSLLGITETLILFLAVIGGMFAGWFTPTEAAAVGALGSLIIAALRRQLSRKILIQSVK
                     ETVRTSCMVMIIVTGANIFGRFLAVTRIPFELASWLAGLPLPGWIIMFMIILFYLLAG
                     CFIDALGLIMLTIPIFFPVVQELGYNPIWFGVIIVTVTQMGVITPPVGVNVYVVGGIE
                     RDVSLQTIFRGAVPFLGAMVLSAVLFVMFPKICTFLPTLVG"
     gene            complement(35065..35595)
                     /locus_tag="PLE88_03550"
     CDS             complement(35065..35595)
                     /locus_tag="PLE88_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOM75576.1"
                     /translation="MDSHSEHDVVRRIRQAYLQFLKGVVFGLTAVSGVAVFVMIGIIC
                     ADVLLRLPCINRPFVGAYDIVRIAGAVALAAALPYTTAVKGHVAIEYFFHKLGPVSRI
                     AVDSVMRLLSIMLFGFLGWQSLLYGRSMYISGQVSQTLQMPLFWLPWIIGICCFAAAL
                     VVVYQLLFPRQEMIKP"
     gene            complement(35597..36697)
                     /locus_tag="PLE88_03555"
     CDS             complement(35597..36697)
                     /locus_tag="PLE88_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOM75577.1"
                     /translation="MKRIIVMAVAAIVVLSLGIAGCSKKTQPPAEPAETTAPESSQQP
                     ASASMPTVKLSYSVFFPPTHIQTKTAEAWAEEIRKRSGGSIEITIYPAQTLTKADQCY
                     DGVVSGISDIGMSCFAYTRGKFPLLEGLDLPLGYPSGTAATRIATAMAQEFAPAELAD
                     VKLLYIHAHGPGILASKKPVRKLEDLSNLKIRATGLSAKIVQTLGATPIAMSQPETYE
                     ALQKGVVDATLCPVETLKGWKQGEVIQYMTDSSIIGYTTAMFVVMNKASWAKLSPQQQ
                     KIVEAVSAEWVDKHGQAWDQADEEGMAFIKELNREIIPLSPQEQQRWKEAVKPILDEY
                     IAAAAAKNLPGKEFLAKIQALIEEQKAAGNAQ"
     gene            complement(36843..37808)
                     /gene="queG"
                     /locus_tag="PLE88_03560"
     CDS             complement(36843..37808)
                     /gene="queG"
                     /locus_tag="PLE88_03560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOM75578.1"
                     /translation="MTIEADIKQKALDVGFDAVGITTAEPIESAQAEYFRRWIEGGNA
                     AKMAYLHRNIQKRFDPGQLLPQAKSVICVALNYRPKPEELVRNPSVRIARFALYEDYH
                     PFIKKRLFELADFIGNSGCTAVQAAARRTRIQFKVCVDSAPLAERALAQRAGLGFIGK
                     NHMLIHPELGNQILLGELITTLELKPDSPPEKQSCGDCGRCIRACPTGALASDGTFDA
                     RKCISFQTIEAPAAAPQPTLPDGSSANGCAPHYIFGCDECLLACPYEAAAPLRKNPDF
                     RIHPEWQNLTAKQIRSMQQEEFAQIFAGSGFIRLGLNRLKQNIGI"
     gene            37913..40765
                     /gene="ileS"
                     /locus_tag="PLE88_03565"
     CDS             37913..40765
                     /gene="ileS"
                     /locus_tag="PLE88_03565"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOM75579.1"
                     /translation="MSDKDGKKGYKDTLNLPQTSFAMKANLTQREPQQRKLWEKEDIY
                     GKIMAARKEAPLYILHDGPPYANGDIHMGHVINKVLKDFVVKYKTMTGFCAPFVPGWD
                     CHGLPIEVKVMAELGEKAKQMSKPEIRRECHKYASKYVKLQTKQFQSLGVFGDFEHPY
                     LTLKPQYEQGILEIFAELVGNGLVYKQLKPIHWSVGCETALADAELEYKDITSSSIYV
                     NFPMDAASAQKVRQMGLAPADSKVCFMIWTTTPWTLAANLAVALHPWHDYVGLTYNRN
                     GTTFTSIVCADRLEAVIKAAGCPEGQYSVSQPVKGSAFEKLRYLHPYIEKNPTDKDAY
                     FAINAEFVTTDDGTGIVHIAPGHGAEDYVAGMQYGLAVYSPVRDDGTYDDTVPQWLRG
                     KKVLEVDTEVNAYLRQLGLLVHEDSILHSYPHCWRSKMPVILRATEQWFISVDKDVEK
                     FGGKSLRNLALEQIKDVTWVPSWGQKRIQGMLESRPDWCISRQRAWGLPIPAFVNSRG
                     QSLLTKESVLAVARHIGKKGSDSWFTDSPKEILGSDFVLPDGFDWDDLRKEENIFDVW
                     FESGCSWHSVIRMAGQPVPVDLYLEGSDQHRGWFQLSLLPALGATGRPPFKTVLTHGF
                     TVDEKGMKQSKSAGNYVSALEEIEKYGADVLRLWVASVNYQEDMRCSDELIGRLQEAY
                     RKIRNTLRYLLSNINDFVPAEMAVDYKDMQVVDQWAMEKLQELIAQVHEAYESFMFHR
                     VYGLIYNFCTVEMSSIYMDVLKDRMYCDGAASRSRRSGQTAMYRILDALVRMLAPILV
                     HTAEEAWAAIPYKSQPVDSVHLALMPQPDASINPSANAGRWGKIMALRDDVLKVLEPL
                     RKNQLIGSNQEAAVQIETNDEELLTILNQIGIETFAALCIVSQVSAAKGAALKITASK
                     SPYPKCQRCWNYWPTVGQNADYPDLCDRCIKVTAGC"
     gene            40940..>42548
                     /gene="recJ"
                     /locus_tag="PLE88_03570"
     CDS             40940..>42548
                     /gene="recJ"
                     /locus_tag="PLE88_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOM75580.1"
                     /translation="MRNIAAGMQKKVWQIRPASRDAAKLAQSLNISPLVAQILINRQL
                     DQTEPARAFLSPKLSDLIAPEQMFGISEAAACIRQAIESRKKISIYGDYDVDGITSVA
                     ILWHLLQMLGAQVDYYIPHRIDEGYGLNSDAVRRLAAGGTELLITVDCGITAVEPVRL
                     AREMGIQTLITDHHQPGAVLPDAAAIVHPRLDPAYGNPHSAGAMVAFKLAWAVANAYK
                     KGVQLSAPLRQYLLNATTLAGIGTIADVVDLRGENRVLAGYGLKALEASNLVGIRTLV
                     QTAELYAADVNSYAVAFRLAPMLNAAGRMGHARLAVELLTTESEVRAIQIAQYLKDQN
                     RLRQKYQLDIFKQARQRIVQMGLHHPDRKTIVLADENWHTGVIGIVAARIVDDFYRPT
                     ILINTGEGIGQGSGRSIEGFNLHAHLEACSDYLISFGGHEMAAGLRIEPSRIAAFAEA
                     FEASAEQAFREKMPASCLTIDAEVRIADFTERLMKELNYLEPFGQGNPKPLFAVRGVK
                     LIAPPRKVGARSDHLQLSIADASGAVRC"
BASE COUNT         9994 a        10490 c        11676 g        10288 t
ORIGIN      
        1 gcatccagca gaaaggcata ttcaaacggc ttgctgctgc gccggcaggt ttggcggacc
       61 tgctcgagtt tgcctttcgg cagggaatcg gtgtcggcca gcagccgccc gataaccgtg
      121 cttttgccgt ggtccacgtg tccggcaatc acaatgttca tccgttcatc tgtatcgggg
      181 cagtgtgcca tactctgtcc ttcttgcagt gtctgaaata atcttacata tacccttccc
      241 gccggagggt ttccagtccg ccgccgtctt cggcatcctg cgcccggccg gaccgctcgg
      301 caatattggc cagctgcccg ctgcggagct cgtcgataat ctcctgcgga tttctggcct
      361 gcgagtcgat gggaaaggtg cagggccagc aacccagaga gcggtagcgc ttgccgtcgc
      421 cctgattgta atacagcgac acggtgggga ttttttcccg ttcgatgtat tcccagacat
      481 tcagctctgt ccagtccagc agcggatgaa tgcgcacatg ggttcccggc gcaaaatcag
      541 tcttgaactg attccaaaac tccggcggct ggtcggcgat gtcccattcg ctttggcggt
      601 cgcgtggcga aaagtagcgc tctttggagc gggatccttc ctcgtccgcc cgcaggccga
      661 caatcacgcc ggtaaacggc tcggtattgg catcagcctc atagtggccg gcggcgtggt
      721 taaaccggta ccgcggccac tgcccgctga gcgtgtgctt aagcgcctcg gtcttgagca
      781 gcttgcagca ggtcagccgg tcggcattgc cgtcagggaa ggtctttttg gcttccagcg
      841 cctcggtgtt ttggccgtaa atcatatcca gattccattg acgggtcagg cggtcgcggt
      901 aggcaatcat ctcgggtatt ttgtaatggg tatcgatatg caccagcgga aacggcacat
      961 gtccgagaaa cgccttgcgg gccagccata aaagtaccgt gctatccttg ccgattgacc
     1021 agagcataca gaggtttttg aagctggcat aggcctcccg caggatgtga atgctcagat
     1081 tttcaagctg ttccagatga tccacgcttt attcctgtaa atagggtttc agatagtcca
     1141 taatctgccg gacggactgt tcaaccgtct gctgttcggt atcgaccacg atttcgggat
     1201 ggagcggttc ttcgtacggg tcgtcgatgc cggtgaactc cctgatttgg ccggccagcg
     1261 ctttctggta gagacctttg acatcccgcc ggcggcattc ctccagcgag gccttgacat
     1321 acacttcgat aaagtttctg cacagactgc ggacaaaatc tctggccgct cgatacggcg
     1381 aaatgaacga ggcaatgacg ataacgccgt gatgctccag acgcgaagcc ataaagccca
     1441 cccgcttgat atgctcatcg cgggcctctt tggaaaagcc ggtgttgggg aatacccccc
     1501 gcaggacatc gccgtccagc cgttcgaccg gtttgcccat ctgctccaga tgattgtaaa
     1561 ttcgctcggc aagcgtactt ttgcctgccc ctgacagccc cgtcagccac agcacgaatc
     1621 cttttttgcc tgttttttcg ctcaaaaatc aagcctcgtc atcaatggcg gcccgaaggg
     1681 ccaacgtcat ccagcccaga gcagcgccct aagggaatca taaacgatac aaaaaatacc
     1741 ctgaaggggc aatatacact tggcaatata accaaataaa cggctaaata caatcccgaa
     1801 ctgccgaaaa caggactttt cggggcggat aaaggattgc ggggtgggag cccgaatttt
     1861 ttttaaaaat tttttttgaa acattttttg gttgacggct tctacatatg tagtaaaata
     1921 tcttctacac ttgtagaaaa tcagaaagga ctgcttatga aactgactga agccgaatgg
     1981 caaattatga atgccctgtg gcaggattgg cccgccaccg cccggcagat tgcagagcga
     2041 ttgccgaaag atgtcaactg ggcttatacc accattaaaa caatgctaaa gcggttgtcc
     2101 gataaaaagg ccgttaaaga gatcaagaat ggcaatgtcg gggtctatga gccgattctg
     2161 acacggcaaa acgcccggcg caacgcgctt aagttgctgg tcaatcaggc gtttgacgga
     2221 gcatttgggc cgctgctgca ttttttatta gaggatcaga agttatcgga taaacaaaaa
     2281 gaggaattat tgcgggtact gcagcaaggc gagcagaaag gagcacacaa tgatccagtt
     2341 ttttaatact atttcagaca agtggttagc atttcaatgg gcaatgcttt ggcagacggc
     2401 ggttttaatc gcgattatct ggatgctgga tgttctgatt cgtaaatggg cgtggccgca
     2461 ggtgcgttat gcgctgtggc tgctggtttt ggttaagttg ttgcttctgc cgacgcttac
     2521 gcgtcgccgg tgagcgtcac atcccagatt ccagacgtgg cgcagcaaat cgtcagcatc
     2581 cagttcaagc ctgtgcctca ggcggcgccg gaccctgcag gggataaaat atcggttttg
     2641 ccggaaacaa actggacgac tatcgcatcc aatagatagc aggcggcaga aaaagctggg
     2701 gtaagccgta atagaaccgc agagccggaa cgcggcagtg atgttctggc ggctgaaatc
     2761 tctgcggctg ttacttcttc tgttgaaacc agcgttgagc aacctgcggc gctctcgtgg
     2821 aaatcgtatc tgatgctcac atggatgctg gggactgcaa tcctgaccgt ttggctggtt
     2881 gtgcggctgt tgggttttgg ccgggagtat ttgaaagacg atggacagta tcaactgccg
     2941 gaacgttttt atgcacagct tcacgctgcc ggagaacggc tgaaactgcg gcggctgccg
     3001 cgggtgatat tggcaaataa ggtttcctgt ccggcggtgt ttggaatttt ttgccccgtt
     3061 ctgctgatgc cggcggaaca attcaggacg atgtcaaatc cggacaccga gcatatcctt
     3121 ctgcacgaac tggcacacat caaacgcggc gacctgattg ttccggccat ttatatggtc
     3181 ttgcagataa tctattggtt taatccgctg ctgtggatga ttcgaaagac gctgcagaat
     3241 ctgcgcgagc tttgctgcga tgccgcggtc gcccgcctgc tgcgggaaaa caccgtccat
     3301 taccgccgga cgcttttgga aacagcccgg cagctcttag ccgaaccggt tgatccggga
     3361 ttgggcctgc tgggtctgtt tgaaaactcc aactggctcg tcacccgcct ccagcggctt
     3421 gaaaagaaaa cttggaaaaa ctgcccgttg cgcatcgcca ccgtgattac gctggtttcc
     3481 gtaatgaccg tctgtgtcct gcctctgggc aaccgaacca aagaacagca attagctttt
     3541 tacatgggct atctgcgaat tgactcgtcg ataggtgata tcagccgggc cgttctctca
     3601 atggagcatg attatgattt cagccagccg tatgcgggct tatgtggaca ggacagcgct
     3661 gccggcaatg aatgaatcct tggctcaatg gtgctgttaa ggctgaatgc ctgtttaccg
     3721 gagccgttta gaagcggtag gtgagcttga cgccgaaggt tctgccgtct tgctcgattt
     3781 tatcctgcag gtgctcggta aaacccggat gctcatagtt gacatccaag aggttgtaca
     3841 gcccgatctg gaagtccaga ttcttcagga tgttgtcata ggtcaatgtc agattggtaa
     3901 tcaccgcatc atcggtatgg ccgccagata gggttttgcg cttgctggta tatttaatat
     3961 ccagtccggc gaagagtttg ttctccagca gcggataaat caggttgaag ttgacaagat
     4021 gagccggcga attggccagc gaagagcggt tggctttatc cagtgtgcga accgatgcat
     4081 aactgctgcg ggtttgaagg ccgtttgccc attttccgat cagcgccgcc tcatagccgt
     4141 cggcggtaac ctgactgagg ttgcgaaact gtgataaccc gtcatccggg tcgatggtct
     4201 gatcgatgag gtcatttact tgatagtgaa aggcgctgaa tgttcccctc cacttctcgg
     4261 agaagcggtt ctcaaaaacg gcctcatagg ttttaatggt ttcaggctgg aggttgggat
     4321 tggctttggc ggtgttgccg ttatcctgat agtaaagttc gtacacgctg ggggtgcgga
     4381 aagcccggcc ggccatcagc ttgaccgtcg ccgtatccgt cagctgatag ataacggccg
     4441 cacgcggatt cagctgagcc cctgtttgat cgtaatagtc gcgccggatt cctgcattga
     4501 ccgtcaggcg gtctgtcagg cggatttcat cctgaatata gatgccccag ttcttggagt
     4561 gtctgcggtc gttgaggtag acatcaaaat cccagttttt ctggtcctgc cggatgttgg
     4621 tttgtgattc ggcaccgaca atcagggtgt ggtgatcgtc gaagcgcttg gttatctgga
     4681 cctcgccggt aatccagcgg cctttccacc agtccttgtt ttcatagagt ccgccgtcat
     4741 cgtaaacata gtggcccctg taattgtaat gatgatagga taaccgccag agaatagaga
     4801 aatcctcgtc aatttgatgg ttataggaca atcctatcag ggtgtagtca tcccacgtac
     4861 gggtgtcttc ggctccgaag accgtttcat aagaggccgt cgggatgcct ttttggcggc
     4921 gtgagccggc agcaaaaaag gtaaaatcgc ggtaagagcc cttcaacagc atattgtgga
     4981 gattttcatc atcgttgcgg gtacggccga agtttgtgga cgggtcgtca aattcgcgat
     5041 aatacagacg gtcgccttcc cagtcggcat aggtgccgga tgccagaaac tcgatgccgt
     5101 tgtcggtctt gcttccgtat gtgaagcgtc cgccggtatg ccggcggctg ccgaatctgc
     5161 cggctatctg gccgccgttg tattggctgc cggattttgt aaaaatgctg acaacggcaa
     5221 acagggcatt gctgccgtac agcgccgagc ccggtccccg cacgatttca acgcggtcga
     5281 tcaaatccgc atcgatcagc gattcattgc ccagcagggc tgtattgccg atattgtcat
     5341 tgagccgctg gccgtccacc agcaccagaa tacgggagtt aaaatccccc ggtctgccga
     5401 atcctcggac gcccatgtaa ctgtaattgc ggtcgtaggt ttcataaaag cccgggacgg
     5461 agtttaaaat atccgttata ttgcggtatc cataccgttt gatttcttcc tgtgtaatca
     5521 ctgtaacaga agaaggggcc tggctggccg gctgggtatg tttggaagcg gagtacacct
     5581 caatatccat cagggactcc agcggcatat tgaaaaaatc ctcctgcgag ctgttattat
     5641 cggccgctga aacaaccgat gcgagcataa acagcaatgc aggaaacacc atccagttcc
     5701 aatttttaaa taaatcgcct cctgttgata aagataaaac ttctcctgct actaacttga
     5761 ataagatgct tatttgtcgg gaatcggcag cccgaggttt acgagttttt tgaagattag
     5821 ctgggcaatt gtgtgtaggg ggctattggc agattcagaa agcggctgat gttttagcgt
     5881 tcgaagcggc ctgatgagcc agccgtgccg gttcgggcag gcggtatttt gtacagcagg
     5941 caagcgtata gtcaacggca tcctgaaaac tgcacaaatg gccgggggag acaaaaaggg
     6001 gttttgtgcc gctgcgggtg cgcaggacgg ccccgatttt atctgcgccg tcatagagcc
     6061 acgaaaagtg cccctttagc aggggcgggg tatcgaatgt gccgatcagg cggcttttgg
     6121 cgcagccgat agtcggggtg ttcagaaaca ggcccagatg gcttgccaaa ccgagccggc
     6181 gggggtgggc gatgccttgg ccgtcaatca ggaataaatc gaccggattt tgcagcttca
     6241 ttatgacttc cagatagacc ggtatttccc gaaatgacag caatcccggc acatagggga
     6301 aaaccagcgg cagccgggcg acggcggttt cgaggggttt gaggtcggga aatgaaaaca
     6361 ccgccgctgc tgcgtatatg gcggtttggg ccttgttcaa agcgcagtcc aagccggcga
     6421 cacaccgcgg ctgttttcgc atcggctgca ggcggacttg gccggccaat gttttctgca
     6481 gctgctttgc ctgtgcagga gagaggtccc attgatgaag attgctgatc ggctgcatgg
     6541 tgttggcgga gaaaataaaa aaggaaccac agcgctcggc tgcgattcct ttttatgctg
     6601 tcctaatgct aaacagacat gtaatcgatt ggctaaaact tccagccgat gccggtgccg
     6661 atagcccagc cgtcgccggt ggcggaaaat tcggttgtcc aatccatatt gttcccgatc
     6721 gggaaaatgg cgccgataaa tccgccgaag ctgctgtctt cttcaaggtc agcagactct
     6781 ttgtaaaccc agcccaagtt atctactgat ttgtaatcat agtctccatt gagatagtaa
     6841 taaaaaggtc cgccgtagag tttccagcat cccatatcca cagtggggcc taaagccacc
     6901 agcagatcaa aagattcgat gtcaatagtg tctttccata aataagttat ttcaccatct
     6961 tcagagcctt tttcgtccca gctggtgtcc agccagttca tttgagcgga aagaccccat
     7021 ttgaccttgt cctgttcggc aaatgtgaag cgggtaccca atccccaagc aaaatcattg
     7081 tcaaagttta taccgccttt ccatgaatcc ccgtcatcat aaacggcatt ccctttcgtt
     7141 ttgacgtcag ccagacccag ttgtgcatag acttcccacc aatctgccag gccgtacccg
     7201 attgcaatgt agtgccgctg ggtcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnngaca ccaacgtcaa accaagccca tttcagcgag gtcttttcga ggtccatatc
     7381 agagtaggtg tagttgtacc cgagattcca ttgtccctgt ttcagctcag cggttggcgg
     7441 gcccataaag ccaactgcca atgccccgac actcatcgac gcaaccagca ctgctaaaac
     7501 aatcattcct ttgttcataa ctactccttt ctaaaaaaat tttcctgata gatattaccc
     7561 gcctattttg taattatcgg tctgctgatt ggcatgtcaa caaaaaataa caaaaaaaat
     7621 atattatata taatatacct gaaaacagca gtattgtttg cagtcagccg gatatggtgt
     7681 gataaaagcc gtttacaagg gcagccaatc aaggtatgat tacctgtttg tgttaatccc
     7741 cgaaaaaagg actctttata ggaattacaa aaagacgtga gtgaagccaa atacatccga
     7801 atcaccggtg cctgtgagca caacctcaag aatatcagca ttgatatccc gagggacaag
     7861 ctggtggtgg tgacagggct gagtggctcg ggcaaaagct cgctggcgtt tgataccatt
     7921 tatgcagaag gccagcggaa gtatgtcgaa tcgctcagcg cctatgcacg gcagtttctg
     7981 gagcagatgc agaagccgca ggtggagcat atcgaggggc ttccgccgac aattgccatt
     8041 gagcagcgca gcgcctcgag caatccgcgt tcaacggtgg cgacgacgac ggagatatac
     8101 gactactgcc gtcttttatt tgcgcgtgcc ggcacgccgc actgctggat ttgcggcaag
     8161 gttatcagca gtcagcacgc cacgcagatt gtcgattcca ttctccaatg gccggctgaa
     8221 acgcggattc aaatctgtgc tccccttatc agcggccaga aaggagagca cagggatgtt
     8281 tttgttcaga ttcagcggga aggatttgtg cgggtgcgtg tggataacca gatttatgat
     8341 gccaaagcgg tgccgctgct ggacaagaac aaaaagcacg atattgctgc tgtggtggac
     8401 cggctggttt taaaagaggg cattcgcatc cggctggccg attcgattga aacagccctg
     8461 aaaatcggcg atggggttgt gctggtgatg aagcaggagc cgagtgcgca aaaaaccgaa
     8521 ggaggcaacg ggggctggac ggatgttgtt tacagcgaaa aatatgcctg tgtggaccat
     8581 ccgcaggcgt ctttagccga gcttagcccg cgtttgttca gtttcaacag tccctatggg
     8641 gcctgtccgt cgtgtgacgg tctgggaaca attctcgaat ttgatccgga cctgattgtg
     8701 ccggacaaaa gtgtttcact tgaaaacggg gctattgagg cgtggcgcaa aggcggaaag
     8761 cggatgaata tttattataa ccggctggtc aagcggttct gccgcaattt caatatcgac
     8821 aaggccgcgc cgttcagcga aatcccggcc gatatccgca agattttgat gtacggaacc
     8881 acggacgctg atgaagcggc ctatggcgtc agctttgagg gtgttatccc caatctggtg
     8941 caccgctgga agcacacgac cagcgagttt gtcaaggagc gtctgcatgg gtatctgtcc
     9001 gaacagccgt gcaagcagtg cggggggacg cgactgcggc cggaggcggc tgctgtgacg
     9061 attggggata aaaatatttg cgcaatcacg cggatgagca tcgatcaggc gtacagttat
     9121 tttaaatcgc tgcggctgga tgaagagaaa acgcagatcg ccgcccagcc gctcaaggaa
     9181 attctggctc ggctgaaatt tatgaaggat gtaggattgg ggtatctgac cttagaccgc
     9241 accagccgca cgctgagcgg gggagaagcc cagcggattc gcttggccac acaggtcggc
     9301 agcggactgg tcggctgctg ttatgttctg gatgagccga cgattggact gcaccgccgc
     9361 gacaatgacc ggctgctggg cattctcaag aagctgcgcg atatcggcaa taccgttctg
     9421 gtggtagagc acgatgatga tgtcatccgg tcggctgacc atattattga cattggtccg
     9481 gctgccggcc ggcacggggg cgagctggtt gcggccggaa cattggatgc tatctgccgc
     9541 tgcgagcgct cgattaccgg tcagtatctc agcggggcca agcgcatcga actgccggcc
     9601 cagcggcgaa aagtgctgat gagcaactgc atcgaggtcc gcggggccga agagcataat
     9661 ctgaagaata taacggttaa atttccgctg ggtgtgctga cctgtgtaac cggcgtttcc
     9721 ggctcgggca agagcacgct ggtaaccgaa attctgctca aagccctgaa gcgtcgtctg
     9781 tacggctcgc ccgaaaaacc cggccggcat aaaagcatca gcgggattaa ccggatcgat
     9841 aaagtaattg aaattgacca gtcgcctatc ggccgcactc cccgcagcaa tccggctacc
     9901 tatacgggtg tgtttgacct gatccgccag ctttttgcga tgacgcgaga ggccaaaatc
     9961 cgcggctaca agcccggccg gttcagcttt aatgtcaaag gcgggcggtg cgagcactgt
    10021 caggggcagg gcacgaaaaa gatagaaatg cattttctgc cggatgtgtt tgtgacctgt
    10081 caggaatgca agggaacccg ctataatccc gaaacgctgc agatcaccta taaaggaaag
    10141 aacatcgccg aggtgctgga tatgcggatt gacgatgcgc ggcagttctt tgccaacttt
    10201 cccaaaatag tgcggctgat aaaggccctc tgcgatgtgg ggctggggta tatgacgtta
    10261 ggccagtcca gcacgacgct gtcgggcggc gaagcccagc gggtgaaact ggcggcagaa
    10321 ttgggcaagc ccggcaccgg ccatacgctg tacattctgg atgagccgac aacagggctg
    10381 cattttgccg atattcacaa tctgatgaat gtgctgcggc ggctgacgga tatgggcaat
    10441 tccgtgattg tgattgagca taatctggat gttatcaaga tggccgatta tatcattgac
    10501 cttggcccgg agggcggcga ggagggcgga cgggttattg cggccggcac gcccgagaaa
    10561 ataatacaaa atgaccacag ctatacggcc cgctatctca aagaaaaact ggccgctgaa
    10621 agggggtaag ccgttttctg ccggccgact ctgtcaggac accgaaacac ccatgtcgca
    10681 tcaacaaaaa cttccattca ttggcctgta ccagcgctgt tgggactcga tacgaacgac
    10741 cttccggtcg ctgcggtatc gcaattaccg cctgtttttc atcggccaag gtctttctct
    10801 tgtcggcacg tggatgcagc aggtggccgt cagctggctg gtgtaccggc tgagtcattc
    10861 ggccgttgcg ctgggggccg ttgcttttgc ggggcaggcg cctgtttttc ttctggcgcc
    10921 ggtcggcggc atcacagctg accgctgcag caagcaccgc attctgctgc tgacgcagat
    10981 gctttccatg gtgcaggctg cggtgctgtc ggttctggtt ttgagcggga ctgtgcaggt
    11041 ctggcagatt gggctgctga gtctgatgct gggaaccatt aatgcatttg atatgccgac
    11101 gcgccatgcc tttgttatcg agatgattga agacagcggc gatctgggca atgccatcgc
    11161 attgaattcc tcgatgttca acggggctcg gctggcaggg cctgcgctgg caggcgtgat
    11221 tatttccatt ttcggcgaaa gcctctgttt tgtgctcaat gctgtcagtt atctggcggt
    11281 tattctatcc cttttgaaga tgcagatagg ccgttttgaa ccgccgtcaa agccggttca
    11341 tcctgttgca cagctcaagg ccggatttgc ctatgcctat cgatttaccc ctgttcgcga
    11401 tgttatcgga tttattgcag caatgagtct ggttggcatg ccctatgctg tactgctgcc
    11461 ggttttggcc aaaaccgttc tggacggcgg accgcagaca tatggtcttt taatcgggtc
    11521 cgccggagcc ggagcgctgt tcggcgctct ttatatggcg tggcggaaaa ccgttcgggg
    11581 cttgggccgg atactggttt tcggcggctc gctgtttggg ggctcactac tggtgctttc
    11641 ggcagccgaa tccgcgggat tttcaatggc tttgctgctg ctgtgcggat ttgggatgat
    11701 ggtcttgatg gccggctgca atacgattct tcaaacgatg gttgatgaca gcaagcgggg
    11761 gatagtgatg agcatctata tgatggcgct gattgggatg gcaccgctgg ggagtctggc
    11821 ggccggtttt atggcacatc ggctggggct gcatctgacc ttgctgatgg ggggattgtt
    11881 gtgccttctg gccagcgggc tgtttgccct gcggctgccg cggctgcggg ggatagtccg
    11941 tccggtctat gttgaaaaag gaatcatcac agaaacggcc ggcagagttc aagacgccgg
    12001 tgagtaggca caagacaaaa cagcagagcg ggcaattgga agtctcacga aatccagagt
    12061 tggggatcgt tttagtgcag cgtccgataa cttaatgtca gctgtatctc cgtaaaaata
    12121 cggacggaaa tttgtaagac acttcccctc aaatctgttt aacaataccg aaaatttatc
    12181 taaaagcggt tttttcatta agccgcaggg tttctttgtc gagaccaata gcagttttcg
    12241 ctgtttgttg ccataattgg tatggtaatg tttttgcagg gctgaccaga gaggtgtgga
    12301 ataaccgccg gcaaataatg gatttagaaa cggctatgaa atcaaattgc ttacaaatcg
    12361 aacaggtaca aatgtctttc agccgttttt tatggaaaag tactgctgcg gcttgttggt
    12421 tttctgtttt gctgacggtt atcgtcggct cggttcaagc cgttgtccag ccgccggcag
    12481 caatcacggc agatttaaat aataagggtg cccaatcgat tgagtacaag gtaaaagcgg
    12541 cctttatcta taacttcaca aagtttacgg tttggccgcc ggaaaaacat caggcatctt
    12601 cggaacagca gaatggtcaa gcaagtcaga ccaagccgct gtttatcggt attttaggaa
    12661 aaaatccatt cgaagatgcc tttgtgccta tattgaacaa ggatgtcggc cgccgcatgg
    12721 aactggtgga aatcggcagc tttgcggagt ttcagcgttc ttacggccgc cgttccgatg
    12781 cgctggatgc ctaccgcaaa gccaatgagc agacgcttgc gaaatgcgat gttttgttca
    12841 tctgcgacag cgaaaaatcc tttgtcagtg aactgattac tctgacagcc ggccggagta
    12901 ttctgactgt cagcgatatg gcgaatttta tcagcaacgg cgggatgatt tcttttgtaa
    12961 aggaggacaa caaagtccga tttgaagtaa atctcaaagc cgcccagaag gagaatctca
    13021 aaatcggctc gcagctgctc ggtttggccc gacaggtaca caagtaacat ctgtgcattc
    13081 agcagggaga caattcatct gacactttaa gtctttgcag gatatgaaaa agctggttat
    13141 tcatacgatt cggaaaaagc tgattggcat cattatgacc atctgcgtgg tgtgtctggt
    13201 gatgtccggc gccgcattca gtctatggag ctatatttcg tttcgcaatg agatgatttc
    13261 cagtatgacg gtgcaggcgg aaatgactgc cggtcattgt tcggctgcgc ttgcttttga
    13321 tgatgcagcg catgccgaaa aaatcctggc ctcctttgca gccaaaccct ccattgtaag
    13381 ctgctgcatt tacaatgcgc aggggaagca ttttgcagcg tacattcgca aagattttgc
    13441 ggatcatcat actgccgtga cgcttcaaaa cgaagggcat cgtatcgaca agggctattt
    13501 gcagatattt atgccgatcg ttcttgacgg ggaaaaaatc ggcacggtgg tgctggtttc
    13561 caaccttgaa ccgctgaagg caaatttccg gaacaacggc tttattattg cagcggttac
    13621 cattgctgcg gcacttggcg ggtatctgct ggcgcgaaaa ttccagcaga ttgtgtccaa
    13681 gccgatactg gagctggcag ctttggcgcg tgctgtccgt gaaaagcggg attatacgca
    13741 gcgggctgcc agcatatccc aagatgaaat aggtattctg accgaagcat ttaaccaaat
    13801 gctggatgaa attcagaagg agatggagga tcgcatcaag gcgcagaacg agctgatcca
    13861 gcatcgcgac cagctggagg agatggtcgc cgagcggacc agtgagctga aatctgccaa
    13921 ttacaatctg gcaatggcgg ttgaaaaggc caatctgatg gctcgtcagg cgcaggaagc
    13981 caaccgggtc aagagcgaat tcttggccaa tatgagccat gaaatccgga cgccgatgaa
    14041 tgccattatc ggcttcagcg agctgctgat tgaagaaccg ctcgatgagc agcagcggtc
    14101 ctttgtgcag accatactca acagcggcca gaacctgctt cggatcatca atgatattct
    14161 ggacttttcc aagattgagg ccggcaagct tcagacggaa atcatcgaat gtgatctgca
    14221 ggggttcttg ggcgacatcg aatcgctctt tagtccagcg gctcgggaaa agggcgttga
    14281 tttcaaggtg ctctgctgcg atgaactgcc ggtcaaaatc tataccgacc ccattcgtct
    14341 gcgtcagtgt ctgctgaatc tgtgcagcaa tgccctgaag tttaccgccc aggggcatgt
    14401 ttatgtcaat gtatccaccg agcgcgacgg gaaggaggat ttcatccgct ttgatgtgga
    14461 agataccggc attggcattc ccaaggacaa gctcaatgcg atttttgaag cgtttacaca
    14521 ggccgacaac agcacgaccc gcaaattcgg tggcaccggt ctggggctga cgattacccg
    14581 ccagctgacc catttgctgg gcggcgatat taccgtccgc agcgaagaag gccgcggctc
    14641 tgtctttacg atgcgtctgc cgatagggac ctgctctaaa aaaccggcta ttgaaccgta
    14701 tccggtactg aatgaaatgg ccgctctgtc cgaagcggct gctcagccgc ctgctccgga
    14761 tccggccggt cggatattgg tggctgaaga tgctgttgcc aaccagaagc tgataactat
    14821 ccttctggag cgcaaaggcc ataaagttac gcttgtggaa aacggccagc aggccattga
    14881 agcggttcag aagaacagtt ttgacctgat ttttatggat atgctgatgc cggttatgag
    14941 cggctacgaa gccgtgcgtc agctgcgggc ggccgggtgc actctgccga ttattgcgct
    15001 gacggccaat gcgatgagcg acgacgaaaa gaaatgcctg cagtgcggct gcgatgagta
    15061 tatttcaaag cccatcaacc gccagaaact ggatgccctc ctgacgaaat acctccgcca
    15121 gccagccgtc gaaagccggt cctgatgaca cgatgcctgc cagacagcga cagagactta
    15181 cgatgacgac ctataaacag tgctttatgc ttctttaacc ttcctgcaac tgattcgcca
    15241 tacgcttcat cccgataggg atgttgggtt tatttttcat ttacaaaatg cattattcgg
    15301 agatgtaata tcctgtaatg tttttaatca aaattaaata aaatatagtt gacattacaa
    15361 ggtatgtgtt acataatata taaagcaata taataatctt tgttttcagg agaaaatcaa
    15421 tgaaatttga acgggaacta ttgaagggcg ttgcgccagt ggtggtcctt gaaattctaa
    15481 gccgcggtaa aatgtacggt tatgaactga gcgaggccct tgaacagcga agccggggta
    15541 ttttgacgct tggccggggt acgctgtatc ccctgctgta taatctggag gccaaaggtc
    15601 tgattgccgc cgaatgggag gaaagcaaat ccgcccgcaa acggcggtat tactccatca
    15661 ccagcagcgg caaggcagaa ctagccgcca aaaaggagca gtggatggcg ttgcagcagg
    15721 gcgtcaatct ggtgtttgga ctgggcagct taagtccggc atagtttggc tggcacggtc
    15781 aagctctgct tggccgtggg gataaaacgc aggagtcaaa catggaaaag aaatgcctat
    15841 cagatttacc gcgggttgcg gtcgaatata tcgattcggt catcaaggcg atgaaatacc
    15901 gccggaatgt acggattgaa gtgcggcagg aattgattga ccattttgcc gatgcgctgg
    15961 cggattgtaa aacaaacgac gaaaagcaaa agctggccga agaattgata gccgaattcg
    16021 gcgatgtcaa actcttgggc acgctgcttc gccgggccaa aaaacgctgc cggccgctgt
    16081 ggcggacgat ggttgcgcgg gcatttcagc ttgtcggcgt tctttttgta ttactggttc
    16141 tgtatatcgg ctggtttttt tccggcaagc ccgttatcac tacgaactac ctggaggtcc
    16201 tcaaccggca ggtacggcct gtcgccgatg acagtcaaaa tgcatggccg ttttataaac
    16261 aggccgtcga aaaatatgtt caatatgaag cgaatgaaga acgggaaaaa tttgattttc
    16321 gaaacccctc gacactgtca acggaggagc ggggttttgt tcaaaaatca atcagcggca
    16381 atcagcaatc gctggacttg atccgccagg gaaatcaaag accttactat tggccggtct
    16441 atgacgtgca ggaaggtcaa aatccggagt taatagcgat atccattccc catttgaaag
    16501 aatatcgaag attagcggaa ctcctgtgct ggcagggatt gctgagcgct gaagcgggtc
    16561 gtttggataa ggcctttcag gaagttctgg agagctactc gttcgggaag catcttcggg
    16621 gtcaacatac gatgctgatt gaacagcttg tagcaattgc cctggaagcg atgtccacaa
    16681 agaccctgcg aattgtctta tcggaatatg gcgaacacat tgacgcagca attctggaag
    16741 ccgcccgaaa acgatttgaa gccgaaattg ccgacgaaga ttttacagct gattttgacg
    16801 gagaaaaatt gtttatatat gatgaaatcc aacgcagctt tactcaatcc cgaatcggga
    16861 aaagtcactt gtatataaaa cgtcttgaac aaaatgtacc cgcgttagct tttggagcac
    16921 aagggaaatt agtgaagcat tggtttcgga ttcttttcac ccatcccggc aaagaggaaa
    16981 cgttaaaaat gagtgaggag ttttattcag ctataaaagc aaacgccgga aaaacacccg
    17041 ccgaaattaa actgctgggt acggaaacta aagccgataa aattgaaaag gaaatattca
    17101 acgacaacat ttttttgggt ctcctgatgc ctgcattcaa tgccgtaata agccgtacct
    17161 atcgaaatcg aattgacagt gaagcaacat taacagtatt ggcgatactg gaatatcaaa
    17221 agcaaaaggg agtactcccg gaaaatctga atgtgctggt tgaagatggt ttacttaaag
    17281 agattcccat cgatccgttc agtgataaac cgttggttta taaaaagacc gacggaggat
    17341 ttacactgta cagcgtgggc ttgaatttta tggatgacgg cggaacccgg agtaaggata
    17401 gtcacgacag gccggctttg tggggcgagg ggggcgatgc agttttctgg ccggtcacgg
    17461 atagttgata cctgttgact gatcactgtt acgcatggtc gaagatcatt tttttgacct
    17521 cgacgccttc gatggcgttc agtttgccca tcagttccgt tgtcttggca tcgtcatccg
    17581 tcatttccag aatcagcaga ccgttgggtg agcagaagcc cttgtctgtc tcatggagac
    17641 ccagacgggt tcggatgctg cagccgtatt cggtcagcag gtgctgtacg gcttcgacgt
    17701 gccgaatgcg gtcggtaata tgaacgccca gaataatgtg ttttgacagc ggcataaaga
    17761 cctccacaaa atcaggtgta aaagaattcg ccgagtttgc ccgacgactg ctgataggcc
    17821 ggcggcgctg cgttcaggaa ttcatccgct ttgcggcaat atttgacaac gccggcaaac
    17881 agcttgacca tattttccaa atcgcccagc gaaacgcatt caggagcgat tctgccggtg
    17941 cgtgcattgc agttgtgata attagccaat ggaatgcata tccccgcggc ggcatagccg
    18001 aagcggctgt acacggttgc ctcgcaggtg ccggcgtcca tcagtttccg ctggtatttg
    18061 aatgtgttgt cttttgactg tattcgtcgg gctgcttcca gcagaaaagc cgtcagtccg
    18121 ggtgaaaaca gactggctcg gtcgccgacg cggacaatta cgccgtcttc cattttggcc
    18181 gctggtagtg cggcgctggt ttccacactg atgatgcgca gcccgctgtg cagccggccg
    18241 ctgcagcaga ggtgcttggc gccatggagg ccggcttctt cggcgaccgt gaaaagagcc
    18301 gttacttttt tgcggatgcg tcgctgcgcc agcaagtcca gcaatgccag aatcgaggca
    18361 cagccgatta aatcgtcgca ggcacggctg tacagacggc tgccgcgtat ccggcaggcc
    18421 ggcaggtccc acattccgac ggcgccttgc cgtacatcgc ctgatacctg cagccatatg
    18481 cggcgtcttc ggaaccgctt gtcagactgc atttttataa tccgggcctt gacgatttgg
    18541 gattgacaaa aaatgcagat gccggcgctg cggaagtatg catccttcag gccgccgtaa
    18601 aaaagggccg ttgtgcgcct gcggtgcgcg tccgtttcga taataaagcc gggatgatcc
    18661 atatgggctg cgaaagccag tttcgagtcc ggataagaag tgccgtaaac ggctgttaaa
    18721 ttgcccacgc ggtcctgatg aatctggatg ctgcgtgcct tgcagaaatc ggcaatgtac
    18781 cggcggacag cggcctcgcg atagggggct gtcggctgat gcagcaggcg tcgaacaatc
    18841 tcaagcactg caggattcat cggcttgcag catttcctcc gccagttcag cagcggtatg
    18901 gacaaatccg tcgcactgtt caaatgcgcc cggctgggcg gccatcattt gtccctgcgg
    18961 cgtcaggaag tcaaatccca gcaggtcgcg gcagtagagc gagccggtgc ggagtttgaa
    19021 ttcttctgtc agcttctgga cctttcgata cagctcatac ttggccgttt tatccattgc
    19081 ttggccgcag ccgtacttga gccccagcac catgaccgca ccggttagtg ccccgcatac
    19141 ctgacccgta cggccgattc ccccgccgaa gcccgaggcc accttcagag ccgtcggggt
    19201 atccatgccg agctcttccg caaaggctgc caatacagct tgggcacagt tcaaacccga
    19261 cccaaagagc tgcaccgctt tttgtgtgtg cttcattgtt tatcccgatt taagttatgc
    19321 aatattccat ccactggctt cgaacggcct gtgccagcgg cttgatggtg tcggccgaaa
    19381 aatcgaacgg gatgcctgct gccgcaaaca gttccgtcag cggccggcta ccgccaagag
    19441 ccagcgctgt tttgtactga gcgatggcct gctggcggtt tttcaaagat gccagccata
    19501 tccccaatgc tcccaactgg gcgattccat actcgatgta gtaaaacgga acctgataga
    19561 aatgcagaat ccgctgccac atcgacgccc ggcagtcttc cagccccgac cagtccacaa
    19621 tgccgtcttc atactgctga ttcaaggcaa gccattgagc gtttcgttgg tgcggtgtat
    19681 gggccggatg ttcatagagc cagtgctgaa aggcatcatt gacagccacg gaaaccagcg
    19741 tgcggacaat cgtctccagc tgctcgcgcc accaccgccg ccgctgggct tgatcatagc
    19801 ataccgacag atgtgcccct gccagcagct ccatggacat cgaagccact tcgcagaact
    19861 ccatcggagc atgccgataa gcccacagcg gctcatccgc acaggccagc gtatgaaagg
    19921 catgtccccc ttcgtgcatc agcacgcaca ggtcatcgtt agtgccgatg gcattcataa
    19981 agataaaggg tttgcgtgcc tcatataagg ttgattgata ccccccgggg gctttgcccg
    20041 ggcggctttc caagtccagc aggccgctgt ccatcatcat cctgaagccc tcgccgaact
    20101 gcctgtccag ctgagcaaac atctgataaa cgccggcagc gaactgctgt gttgtctcaa
    20161 acggccgcag cggcgcccgc ccgagaggat ccgtattcag atcccacggc cgcagtttgt
    20221 ccaagcccat ctgttttgcc cgatagccca gcatttcctt caaaagcggg acaagatgct
    20281 ggcgcacagc ggcatgaaac tgccggcagt cctgcggggt gtaatcgaac cggtgatatt
    20341 ccctgaactt gtagtccctg taattgctga agccggcgtt ttcggcaatc cggctgcgga
    20401 cagaaatcat tttgtcaaaa atatcatcca gctggtcttt atcctgcaaa taccgccggg
    20461 aggcagccat ccaagcccgc tggcgcagag gacggtcggt ttccagcaga aatttgcgca
    20521 tctgtacaat cggccagatg cggccttcaa agggcactgt cattgcaccg gttagagtct
    20581 gatactgctg gctgagcagg gcatcctgcg tctgcagagg cacattggca tcgcgataca
    20641 gttgtacatc gctgcggacg gtgcggctgt aaacggcgta gcgaggcggc tgatattgca
    20701 gttgttcggt cagcaggagc catttgcggt cgagtttgtc agaaagaggt tttaccgccg
    20761 gaaccactgt gtccataaaa gtccgataag ctgcagcccg ctgcggatca tcggtttggc
    20821 aggtcattcg gatataaaga atgctttgac actgagcgac agcggcttcc agctccgagc
    20881 ggtcggccag ccactgctcc agctggtctg tattatctat cggcctatcg gccagctggc
    20941 ggtaaagggc cgcgaccgaa tcccgatttg tcagctccag tccggccgga acaaaccgac
    21001 gcggctgata aggaggcagc gtttccaaac tgatatcgtt gtttttgctc gcggagctgt
    21061 ctgctttttg gttattcact tttatcattt aataggccgc cggtttttta gggtgattat
    21121 atcacggttc caatctttgt aaatctgtct tatcgccggc aaataatccg cagtaaccag
    21181 caggcatcat ttgaacgtcg ccttcactgt cggtttggtt agttgtatca gcgtaaagat
    21241 gcctaaaatt gttcccagcg gcacacatag acattccaag cccgcgataa tttgacaata
    21301 aaccaaatat ttgcgggtgc ttatatatcg tccggccagc agcaccagag tgccaaaaat
    21361 acaggtaagg ggcaaaatca gcagtaaagc tgcaaagaag gtcaagaaca aaacaaacaa
    21421 gttggtatca tgcggactat gcgacagagc aactgccgca atgccgataa tgaatggtgc
    21481 gattaaacct ttgaagtaaa tcaacacggc cagcacataa tgaagtattg aaatcgtttc
    21541 caaataattg gcattacttg tttcagtttc cataggaatt gctccaagag aatatttata
    21601 ttgcagccga tttgaaaatt gtacagatgc aggaattcaa ctgcaatata aaatgctcgt
    21661 tatatgtgga caaagcaagt tcgcaattat ttttttgttg atagtaatga acagttgtta
    21721 tactatgtga gtatatactc atatagacac aattattgtg gatatttcaa atgcagtatt
    21781 tatatgaaaa acaagctgcg attgccaagg caattgccca tccagtgcgg gtggcggtac
    21841 tggattttct ccgtactggc gaacaatgcg tttgtgacat cgctgcggca gtcggttccg
    21901 aacgaacaaa cctttccaaa catctgtcgg tgatggtggc ggctggcgtg ctcgaatccc
    21961 gaaaggatgg gctaaaggtg atgtatcgca tcaagacacc ttgtgtactg cggtttctcg
    22021 aatgtctgac agggtgcctg cgggaacagg cggccgagca gaggaaattt ctggaagcca
    22081 tacattaaca gcatacttaa tcaataagct gaggactctt atgaatctca aggcggaatg
    22141 gaaaaagctg gcatggatta tcagtgtttt tttgctgtgt ttttatctgc cggtgggtgt
    22201 ggggcgtttt gataatgccg tcagtgaagc cctgtcgctg actaaatggt atgcacggga
    22261 gcatgtgctg ctctgtctgg ttccggcgtt ttttatcgcc ggagcgattg gtgtttttgt
    22321 cagtcaaggt tcggtgatga aatatttagg ccccaaggcc aacaaggttt tggcctatgg
    22381 tgtggcatcg gtctcgggga caatattggc tgtctgttcc tgtacggtgc tgccgctgtt
    22441 tgcgggcatc taccgcatgg gtgcgggttt aggaccggcg gtggcttttt tgtattcagg
    22501 accggcgatt aatattctgg cgattattat gacagcccgc attcttggaa tggaaatggg
    22561 tatagcccgt gcggtggggg cgattctgtt cagcattgta attggggtat tgatgcatct
    22621 gatttatcgt aaagaggaaa tcgccaaggc cgaggcggcg atggctgtgc cgcaggccgc
    22681 atccagccgg cctttgtgga aaaatgcggt tttttttgcg gcaatggtgc tgattcttgt
    22741 ggcggccaat tgggcacgaa gcggcgatgt acgggctttc tttttatgct gccccggcgg
    22801 cttgcagacc tacgccgtgg agggcaagct tcttgaacaa acggatacaa cggtaacggt
    22861 agtggacaat acaggcacaa aacacgtgat tcctgttgac cagtttaaag gtgttgagcc
    22921 gattaagaaa aatccggtct atgaatggat atctcaggcc cgttggggca ttgttgccgc
    22981 ggcaggggtg cttctggcgg ctatgtgcat cttctggttt accaagccgg aactgattga
    23041 gtgggttgat gcctcttgga cttttgccaa gcagattctg ccccttctgc tgataggtgt
    23101 gctggcggcg ggatttttcc ttggtcggcc gggcaaggaa ggaattattc cgaatatctg
    23161 ggtagaaaag gctttgggcg gcaatggcct tggcgctaat ctttttgcgg cggtggcggg
    23221 agcatttatg tattttgcca ccttgacaga agtgccgatt cttcaggggc tgatgggatc
    23281 cggcatgggc aagggaccgg cattggcgct gctgctggca ggacctgccc tgtcgctgcc
    23341 caatatgctt gttatccgct caataatggg cacacagaaa acagttgtgt atgtgctgct
    23401 ggtgattgtg atggcagcga tttcgggaat gctcttcggg gcgtttttct aaaggaaaaa
    23461 aatggtagat aaggccgatg aatttactgt caaggcgata ggcattattc ggacaccttt
    23521 taagcatccg caggaggctc cgattcagcc ggcgtttgcc aaaggtgccg aaggtgtcgt
    23581 tgacatattc gagccgtatg aggcggcact gaaagatttg gacggctttg agcggatttg
    23641 gctgatttat tattgccacc gggcggccga gtggaaaccg ctgtccaagc cctatctgga
    23701 tacagttgag cggggactgt ttgctacacg ggcgccggcc agaccgaatc ccatcggcat
    23761 atcatcggtg cgattggtgc ggatcgaagg ccgccggctg tatgtccggg atattgatgc
    23821 actggacgag acgccgctgc tggatattaa accctattca ggcagatttg atgtttttga
    23881 gaccaagtat aacggctgga tggatgcggt cgatacgagc aatccgaagg ccgatggacg
    23941 tttttggaaa ccgcagacaa gtgaataaaa gggataggca atgaagaaga tacaaatatt
    24001 aggcacgggc tgtccaaaat gcaaaaagct ttcggaaaat gcggaagcgg cggccaaaga
    24061 aatggggatt gaatatacgc tcgaaaaaat tacccagatt aatgacatga tgaagatggg
    24121 ggttatggta actcctgcgc tggctgtgga tggggttgtt aaaacggccg gcaaagtgtt
    24181 gactgttgag gaaataaaaa agcttcttta acaaagcaaa acattgaaag gatacaaaaa
    24241 tggcggatcg agaaagctgt ctgtgttcgg gcggacccaa actgattttt gcttgttccg
    24301 gggcagcgga tgtaggtgca atagccgacc aagcggctcg aaaaatgtca aaagacggca
    24361 tcggcaaaat gttttgtctg gccggcatag gcgggcgcat tgacggcatt atgaaaacaa
    24421 ccgaagccgc ctccatgatt ttggctatag acggctgccc gctgaactgt gttaaggcct
    24481 gtcttgagaa ggccggtttt accgcattca agcatcttca gcttgcggat ttggggctgg
    24541 aaaaaggcaa gagcccggtt acagaagaag ccgtcagccg ggttgccgac aaagccaagc
    24601 tgatgctggc atagcacaac ggcggcttta tggaacctct gccaattgac aagacagttc
    24661 aatgtccctg tgacagcggc ggggcgcatc cctttttgct tctggtaatc tttgcaggag
    24721 catggctgtt gttttactgc ataaaaatgc tgattaggtc caagggagta ttgccgatga
    24781 ataaaatgca taaaataatg attgtaatgg cgctggcggt tctggttggg gttgtcattg
    24841 tcatgaaaca gcaggagccc caaagccctg ctttgcagaa tacctcgaca cagcaagctc
    24901 aaacagccgt atctgaatta ccggtagaaa acaaggctgc ttctccgatg ccggcagcaa
    24961 agcccggtac gctgccgatg ctgctggatt tgggggctga caagtgcgtt ccctgcaaga
    25021 tgatggcgcc gattctggag gcattgaaaa aggaatatga gggcaaactc catgtggtat
    25081 ttatcgatgt gtgggacaat cccaatgagg cctcacgata cggcattcga ctgattccta
    25141 cgcagatttt ctatgatgcc ggcgggcggg agctgtttcg acacgagggg tttatctcta
    25201 aagaagatat tctgaccaag tggaaggagc tgggcgtgaa tttggcagat gccgggggta
    25261 tgccgccggc atttgagcgg cttgtgccgg cccgggcgga tacgcggccc aaagaacaga
    25321 tttgctatat gtgtgacggg gatatttcac cccaaacgct ggtgacggtt aaaactgaca
    25381 agggcgatgt gcggctatgc gggctgcact gctattttat tatgtactcc tgcttaatgg
    25441 aagacaagac ggattttgaa aacaaagtga cggtttcgga ttttgaatcc ggcaggccgg
    25501 tgccgctgac cgaagccgtc tttctggcag gaaccgatgc caaaggccgt ccgatgctgc
    25561 gggcttatgc aaatgctgat gcggccaatc aggcccgcca gaccgacggg ggcaatgtga
    25621 ttggtttgca ggcgcttcgg cagaatgaac tgtcctgccg atgcggattc tgcgaccgtg
    25681 cgtgctatcc gcaggacgcc gccgaggtga ttgttgaagg cggactgcgc acgtggggct
    25741 gctgctcgca ttgtgcgctg ggggtagctg cccgaacggg caaggatatt gaaattcacg
    25801 aaaaagacag actgactggt gagccgatta ttgtcaaaac gctcaacggc agcatcctgt
    25861 cgctggatcc gcctacagca gtggcgtggt tcgggcagcg tcagaaaccc gacggatcct
    25921 ggacctctgc cggctgcttt catcagggat tttttgtcaa tccggataat ctgataaaat
    25981 gggtcgaaca gaatccctat gaaaccggca gacttatcag cattcatcag gcgctggccg
    26041 ataagatgaa gctgtcgccg gaacagatac agaaggcctg caaaatcggt gaatgtacgc
    26101 cgaaatagcg gccggcacag taaatctgca ggagaatccg tattcatgca ggacttgttt
    26161 acacaactga ctcgtgcagt cgagggcgca tggtttattg cggtgcctgc ctctttgatc
    26221 tgggggattt taagtattct actcagtccg tgccatctgg ccagcattcc gctgattgtc
    26281 ggctttattg atggacaggg tagaatttct gccaaacggg cgttttggat atcgactgtt
    26341 tttgctgtgg ggattcttat cacaattggt gccatcggag ccattacggc cgccttgggc
    26401 agaatgatgg gcgatgtggg cagatacggt aattattttg tggcgctgct attttttgca
    26461 gtcgggctgc atctgctgga tgttatcccg atgccggggt ccgggccggg gcagattggg
    26521 ataaagcgta aaggaatgct ggctgcattt attttagggc tggtgttcgg cattgcgttg
    26581 ggcccctgca cctttgctta tatggcgccc atgctggggg tcacattcaa gctggcaatg
    26641 accaatttct catacggtgt tgttctcttg ctggcctacg gcattggcca ttgttccgtt
    26701 attgtctttg cgggtacatt tactgaggtt gtacagcggt atatgaactg gaatgagaaa
    26761 tcaaaaggcg ctgataatct taaaaaaata tgcggtctgc tggtgctgct gggcggggtg
    26821 tggctgattt atacggctta aacggggctt ggcaaaaagg gaggataatt taattgattt
    26881 ttgccgattg atatataaaa tagcattcag acggggatgg agttccccct ataaccgctt
    26941 caaaaagctg ataactcctg caggaactgc tgcgggagtt ttttattgac tcacaacgcc
    27001 ccgcagggaa caataacacg agcagtatta cctaaacgcc ggagattaag gatgttgcag
    27061 aaattgttat ggctgggtct tgccggggca gccggaacat tgacacgcta ttggctgtca
    27121 gggctggtcc aaaaaaatgt ttcggttgat tttccaatcg gaacaatgac ggtaaacatt
    27181 gtcggctgtc tgctgtttgg tctgctgtgg gcttttatcg agagccggct gtctatcagc
    27241 ggccaaatgc gtatgattat ttttctggga tttttcgggg cctttactac attttcgtca
    27301 tttgcttttg aaaccagtca gctgctggat gaatcgcaat ggctgtgggc agcgggcaat
    27361 atcctgctgc agaacattct gtgccttgcc ggtatgatta ccggcttgac ggttggcagg
    27421 tggatttaat cttaaaaagg agaccggtta tgcaattgcc ctcggaagca caattgctgc
    27481 gaatctttat cggagaagcg gaccgtttcg gcgggaaacc gctgtatgaa gcgattgttc
    27541 atctggcccg ccaaaagggc atggccgggg ctacagtagt aagagggttg atgggtttcg
    27601 gtgcgcacag ccgtatgcac acagccaaaa ttcttcgcct ctcggaagac ctgccgatag
    27661 ttattgagat tgtcgataag cccgaacgga ttaaagatct tcttgccgaa ctggatggga
    27721 tgataaaaga gggactggtg acgctggaaa aagtgaccgt atttgcctac cgctgcgaaa
    27781 atggaaatcg taagtttatt tagcggtgac tttttaaatt atggaataga caggacagta
    27841 tggtactgcg caaattacga attagattac tgcgattggc gttccttccg attgtttttg
    27901 tggcagtttt tgtaagaccc aaatagtctc tggattcatt taccgctttt tttgtttctg
    27961 agatatctgt tctggatcag catggaatta tactggtgct gtggcatttt ccatagagat
    28021 tattttgtcg ccgattgaac agaagggaca gttctttatg aacaatcatg cccatagacg
    28081 ggcgatgggg tttgtgctgc tgctgggtgt tgtctcgctg tttgcggata tgacgtatga
    28141 ggcagcgcgg tccctttcgg ggccgtatct ggcgattctg ggggccagcg gggcgattgt
    28201 cggctttacg gcaggcgcgg gtgaactgct cggctacggc ctgcggtttt tttcgggcct
    28261 tttgagcgat aagacgcagc gttattggct gatgaccatt gccggctatg cggtgaacct
    28321 cttagccgtc cccttgctag cgctggctgg tcactggggc tgggctgtcg gtctgataat
    28381 ggccgaacgg ttcggcaagg cgcttcgcac gcccgcacgg gatacaatgc tgtcctgcgc
    28441 cacatcggtg gtggggcgcg ggtggggttt tgctgtgcat gaggcgatgg accagatcgg
    28501 ggcgatggcc ggtccgctga ttgtcatgat tgtgctggcc gtcaacagct cttaccggtt
    28561 cgcgtttgcc gttctggcga ttccggctgt tctggcgctg agtgtgctga ttgcagcgcg
    28621 cgttatattt ccgcatccgc ataaactgga agaggaaatt ccgcagcagg gggacggaaa
    28681 atttccagcg gttttatggc tctatctggc ggccgttggt tttgtggcgg caggttttgc
    28741 cgattacccg ctgattgcct ttcacatcaa atcgcatgcg ctgctggcgg acaaatggat
    28801 tccgctgctg tatgcctgtg cgatgggtat tgatgcgctg gccgctttag gctttggacg
    28861 ctggtatgat aaaaacggcg ttctggcact ggccggagcc gtgattattt catcgatttt
    28921 tgccctgttt gccttttcgt ttagacctgc cgcagtcatt gcgggcattc tgttatgggg
    28981 tatcggcatg ggcgcgcagg aatctattat ccgggcagct gtggcggata tggtgcccag
    29041 acaccgccgc ggcacaggat tcgggctgtt caacagttgc ttcgggattg cctggtttgc
    29101 cggcagcttt ctgatgggca ttctctatga tgtttcgctg cccgccctga ttggattttc
    29161 gattgcggca caactggctt ctctgccgct tcttctggct gtccaaaaac gcctcagccc
    29221 gtcgaagtaa gctatggcct tacggaacgg ctaaaccagt tctgtgtgcg gaggcagatt
    29281 ttaaccagca tcagcatcac gggcacttca atcaaaacgc cgacgacggt tgccaaagcc
    29341 gctcccgatt gaaggccgaa cagcatcgtg gctgtagcaa ttgcgacctc aaaatggttg
    29401 gatgccccaa tcatggccga gggagcggca tccggataag acaggttcag cattttggac
    29461 aatccataac caattgcaaa aatcagacag gtctgaataa acaatggaat tgcaatccac
    29521 agaatggtca gaggattatt caaaattgtt tggcctttga aggagaacag cagcaccagc
    29581 gtcagcagca gggcaagaat agtaatcggc gtcaggatgt gcagaaattt ctgctcaaac
    29641 caatcagccc ctttatgagc aaggatccat tttcgggaaa taaaaccggc cgcaaggggc
    29701 agcgcgacat aaatggcgat ggacaataat aaggcctgcc acggcaccgg cagtttgccg
    29761 acccccagaa gaaaaccccc cagcggccca taaagaagca gcattgtcaa tgaattaatg
    29821 gccaccatta ccagcgtatg cccgtcattg cctttggcca gataccccca gaccagcacc
    29881 attgccgtac acggtgcgat gccaagcagg atgcagccgg ctaaataact tctccaaagc
    29941 ggaacttgca gcattttgac gccgtttttc agtacgattg tgccgacgcc gtattctgcg
    30001 cctaccggca gatccagccc caacggcatc ttgacccaat cgacagcatt cgggccgata
    30061 aactgataaa acaaagtacc gaggaaaaac aacgctatcg catacatggt aaagggtttg
    30121 attgcccagt tgataaacag cgtcagtcct accggcttga tgcttttgcc ggctttaagg
    30181 acttcggcaa agtcaatctt gaccatgatc ggatacatca tcaaaaacag acaggccgct
    30241 atgggaatgg agactacagg tgccccattg acatagatgg ccatgccatc cagagatgcg
    30301 gccaagcccg gtgccagctt gcccatcaca atacctccca gaatgcacaa aataatccag
    30361 acggtaagat acttttcaaa tatgttcagc ccttttgctg cttgcggatt agacattgct
    30421 actccttgtt atggtgcggt atctcttttt ccagcagttc atccatttgc cttgcggctc
    30481 ctgtccggac cattttgccg atatgaacgg cctgctgtat ttctgtatgc ccaaggccca
    30541 tctgctgtgc tttgttcgtg tggaatatga cacacggatg acagttgcac gccactgatg
    30601 cgccgagggc aatcagttcc ttggtggcgc tgctgacagc tgccgaatgg tccttatacg
    30661 cgataaaatc tgcgctgaca atgtaatcgc tgagtttctt gtctgccggc agatgttggc
    30721 gaatcgactg atacaaagaa tcattgcagc tggccatcct atccatgtta gatggtttta
    30781 cagcaacctg cggatgaacc agacccgccg cagcaatcat cgcctgcgtc cgctcgatca
    30841 gaactgctcc ccccacacag ccgataaggg cagcgattga ctcctgaacc ttttgaggca
    30901 acggattggt cagtgccaaa tcggagatgc aggccttgcc gccgggtttc aaaacacggg
    30961 caatttcctt ccagacctgc tgcttgtccg gtgagagatt aacgacacag ttggaaataa
    31021 ccacatcgac cgaggcatcc gctgccggca gatgctcaat ttcccccaga cgaaattcca
    31081 cattggatag ccccgtcttt tctgcaaagg cttcaatgcc cgccctcgct ttgctgacca
    31141 tctcggcagt catgtcgacg ccgataacat agcctgtcgg accgactttg ctggcggcga
    31201 taaacacatc cagtccgccg ccgctgccca aatccaagac aacctgaccg ggcttaaggt
    31261 cggcgatagc cgtcggattg ccgcaggaaa gccccatatt agcgccctgc ggcactatgt
    31321 ccaattcctc ccggcgatag ccgatgccct cagccagctt gttcggctct gctgaaccgc
    31381 agcagcagcc cgattgacct cgggcaatat cggcataccc cagacgcact gcatctctga
    31441 ttttattatt tcccttgcca gtcataaaaa ctccctattc taatccgttt tgtctggata
    31501 gttgtgcatt gtcagccaat tctgaacgcg ctgcttaact gcatcgcgcg tctggcgaaa
    31561 ggcgtccact acctcttcct ttggaccggt tttctttgcc gggtctgcca aaggccaccg
    31621 ttcggtatgc agtgccaaag gcatcaactg ggggcattcc gcctctccct cctgacaaac
    31681 gataataacc caatccagca gttctttacc cagatacaag tcgatgcttt tggcagtctg
    31741 accggcaata tcaatgccgt cttcctgcat gacaagataa accagcggat gaatcatctg
    31801 gggataaagg ccggcgctga tggcctcaaa ttgatccggc gccagtttct tcaaataggc
    31861 ctcggccatc tggctgcggc aggaattgtg cttgcaaaaa aataacacgc gctgtttcat
    31921 aagccgctcc tttgatcggt taatgcttcc ggcagagtct caatgaactg cctaatttca
    31981 tcccgtatgc ggcgatagca gttcagtgcg tcctcttctg tttttgcatc ttttgccagt
    32041 cgcgggggat cttcaaaatt gatatggata ctttttgcct tgccggagaa cagcggacag
    32101 ctttcccgtg cattatcaca gacggtaatt acataatcga aatccattcc cgctaattca
    32161 tttagatgtt tagagcgatg ttgtgaaata tccactcctg cctctgccat tactttgaca
    32221 gcgcagggat tcaatccgtg tgtttcaatc ccggctgaat acggttcaag tatgtcccct
    32281 tttaagtgtc tggcccaccc ctcggccatc tgactccgac aggaattgcc cgtacataaa
    32341 aagaggattt tcagtttatt cttccccttc tgcggcactc tgcagttgga ttcccccagc
    32401 cgtttgagat cttgctgtgc ttgcggatgg tcttttaaat aatcgcgaaa gaaattgtgc
    32461 agggattgct caaacattgt tgctgcaggg gataatcggt agtgcatcca agtcccttga
    32521 cgctgcgcgg taaccaatct tgccttgcga agctcaccca aatgccgcga tatcttaaac
    32581 tgcggttcat taagcgcatc gacaagctgg catacacaca actggccgct ttgtgccagc
    32641 agtgtcatca accgaagccg aatgggatcg gcaagaacct ttaaaatggc cgcttcttgt
    32701 ttcatttata tgctcctttt atgcatatat gtatatatac gcatatgtat gacaaaaaag
    32761 tcaatagaaa attataaaat ttagttaact tatagctttt ttatgtcctt tttgctgtcg
    32821 ataataagtg ttaccggacc gtcattgacg gcatccacta tcatatacgc tgcgaaaact
    32881 ccggtttcga cggccaattg acttcttcgg aggacatcga ttgtttgctg gtaaagtatt
    32941 tttgccacct gaggcgtgcc ggcagcggag aaatccggcc gacgccctct gcggcagtcg
    33001 ccgtataatg tgaactggct gaccagcagg atgccgcctc cgatgtcccg aaccgacagg
    33061 ttcatttttc cgtctttgtc ttcgaaaatc cggaggttgg ttattttgtc ggcgataaaa
    33121 tcaatatctg cgcgggagtc gtctctgccg atccccagaa acaccagcaa tccttgttcg
    33181 atgcggccgg tgatttgacc ctctgcgctg acagatgctt ttttgacgcg ctggatgaca
    33241 gcccgcattg ctcatccttt ttcttgaagc cgcttttgat agctcagcgg caccaccagc
    33301 acagcacaat cggctttgcg gaccaccttt tcattgacat tgccgaatag cgtcttctgc
    33361 aatgcgccac ggccctgccg ccccatcaca atcaggtcta tttccatctc tttggcaaat
    33421 gccacaattt cctgccagtc ttttccgatg cggtattcga tatgcagttt caatccctcg
    33481 ggcagtttgg atacataggc ctctgctatt cgctcatcca agtcgcgttt ggccttgtta
    33541 tcaacgtttt cgacctcgta gatgtaggtt ttccagaact gggcttctgt ttcggggatg
    33601 acatgcagca gatacaattc gctgcccggc cgccgcattg ccgcgtccac tgcaaattgg
    33661 aaagcgaaat ccgcattttc cgacagatcg gtgcagaata gaattcgcct gtaggttgtt
    33721 tgtatttggt tttgcatacc atttcaccgc ataatgaatt atttgctcag ccgaccagtg
    33781 tcggcaggaa ggtgcagatt ttcggaaaca tcacaaacaa aaccgctgat agaaccatag
    33841 cgcccaaaaa tggaacagcg ccgcgaaaaa tagtctgcag cgatacatcc cgttcaatcc
    33901 cgccgaccac atagacattg acgccaaccg gcggggttat gactcccatt tgcgtaaccg
    33961 tcacaatgat gacgccgaac cagatggggt tatagcccaa ttcctgaacg acagggaaaa
    34021 agatgggtat ggtcagcata atcagcccca gcgcatcaat gaaacagccg gccagcagat
    34081 aaaacaggat aatcataaac ataataatcc atcccggcag gggcagccct gccagccagc
    34141 tggccagttc aaacggaatt cgggtaactg ccagaaaccg tccgaaaata ttggctccgg
    34201 tgacgataat catcaccata caggaggtgc gtacggtttc cttaaccgac tggataagga
    34261 ttttgcggct taactgcctg cgcagtgccg caataatcag actgcccaga gccccgacag
    34321 ccgcagcttc ggtgggggtg aaccagccgg caaacattcc gccgataaca gccaaaaaca
    34381 gtatcagcgt ttctgtaatg cccagcagag aaacaatttt tgccccccag cccagtcgcg
    34441 gaccgcatgg ccccagtacg ggattgattt tacattttat gtagattgtc aaacagaaca
    34501 gcagggccat cagcagcccc gggaaaatgc cggcgataaa cagttttcca atggattgtt
    34561 cggtcagaat cgcatagacg atgaatacca cgctgggcgg aatcagcata cccaaggaac
    34621 cgccggcagc aacagccccg cagcttaact cggggtgata tttgtaccgc ttcatttccg
    34681 gcagggctac ggcggccatc gtagcggctg tagccggacc ggaaccgcaa atagccccaa
    34741 atgctgtaca ggcccctaca gtggatatcg ccagaccgcc gggcaaatgc cccagccagt
    34801 gataggccgt atcaaacaga cggcggctga tgcctgcatg aaaggcaatc tgccccatga
    34861 aaacaaacat cgggatgacg gtcagactgt attgggtgaa tgtttcatag agttctgtcg
    34921 tcatcatggc tgtggcggcc ttgggcgtta caatggccgc aaagcccgca aatcccacaa
    34981 tcgccatcgt aaaggccacc ggcatgctgg ccgccagcag cacaaacagc accgcacatc
    35041 cgatgattcc atactgcagc ggtgtcatgg ctttatcatc tcctgccggg ggaacaagag
    35101 ctgatataca acaaccagcg ctgcagcaaa acagcagata ccgataatcc acggcagcca
    35161 gaacagcggc atctgcagtg tctgtgaaac ctgcccgctg atatacatcg accggccgta
    35221 caggagactc tgccagccca gaaagccgaa cagcataatc gacagaagcc gcatcacgct
    35281 gtccaccgca atccggctga cagggccgag cttgtgaaag aaatattcga tagccacatg
    35341 gcctttgacg gctgttgtgt agggcagagc agcggccaaa gctacggctc ctgcaatccg
    35401 cacaatatca taagccccaa caaaaggcct attgatacac ggcagacgaa ggagaacatc
    35461 cgcgcagatg atgccgatca tgacaaaaac agcaactccc gagacagcgg tcagcccaaa
    35521 caccactccc ttaagaaact gcagataggc ctgtcggata cgccgcacca cgtcatgttc
    35581 gctgtgggaa tccattttat tgagcgtttc ctgctgcctt ttgttcttca atcaaagctt
    35641 ggattttggc cagaaattcc ttgcccggca gatttttggc cgctgctgca gcgatgtatt
    35701 catccaatat cggcttgaca gcctctttcc accgctgctg ttcctgaggg gaaagcggga
    35761 ttatttcacg gttgagctct ttgatgaatg ccattccttc ctcatcggcc tgatcccacg
    35821 cctggccgtg cttatcgacc cattcggcgc tgactgcttc aacaatcttc tgctgctggg
    35881 ggctgagctt ggcccagctg gccttgttca ttaccacaaa cattgcagta gtgtagccaa
    35941 taatggagct gtcggtcata tattgaatga cctcgccctg tttccagccc ttgagagttt
    36001 ccaccgggca cagcgttgca tccaccacgc ccttttgcag ggcttcgtag gtttccggct
    36061 ggctcattgc gatgggtgtt gctcccagcg tctgaacgat tttggctgaa aggccggtgg
    36121 cacgtatttt cagattcgac agatcttcca gcttgcgcac cggcttcttg gaggccagaa
    36181 tacccggacc gtgagcgtga atataaagca gcttcacatc ggccagttca gcaggcgcaa
    36241 attcctgcgc cattgcggtt gcaatccgtg tggcagctgt gccgctcgga taccccagcg
    36301 gcagatccag accttccagc aaagggaatt ttccccgggt ataggcaaaa cagctcatcc
    36361 cgatatcgga aattccgctg accactccgt cataacactg gtcggccttg gtgagggttt
    36421 gggccggata aatggtgatt tcgatgctgc cgccgctgcg cttcctgatt tcctcggccc
    36481 aggcctcagc cgtttttgtc tggatgtgtg tgggcgggaa aaacacactg tacgacagct
    36541 tgacagtcgg cattgacgcc gaggccggct gctgggatga ttccggggca gtcgtttcag
    36601 ccggctcagc cggcggctgt gtctttttag aacagcctgc gatacccagc gataaaacaa
    36661 cgattgcggc aactgccatt acgataattc gtttcatgac attacctcct gaaagttgaa
    36721 tagtttctgc tgtgcattct gcacacagtt atttcctatg ctgctatgct aagcattcaa
    36781 atccctcgat gcaagagcaa ttttatcacc aacaagactt ttttcagatg cttttttagt
    36841 cttcatattc cgatattctg cttgagacgg tttaagccca gtcgaataaa accggaaccg
    36901 gcgaaaattt gggcaaactc ttcctgctgc atgctcctaa tctgcttggc tgtgagattc
    36961 tgccattccg gatgaatgcg gaaatcggga tttttccgaa gcggggcggc tgcttcatag
    37021 gggcaggcca gaaggcattc gtcgcagccg aaaatataat ggggagcgca gccgttggct
    37081 gagctgccat cgggcagcgt gggctgcggt gcagctgcgg gtgcttcgat ggtttggaag
    37141 ctgatgcatt ttcgggcatc aaaagtgccg tctgaagcca gtgcgcctgt cgggcaggcg
    37201 cggatgcacc ggccgcagtc gccgcaggat tgcttttccg gcgggctgtc gggtttcagt
    37261 tcaagcgtcg taatcaattc gcccagcaat atctgattgc ccagctcagg gtgaataagc
    37321 atatggtttt tgccgataaa gcccaatccc gcacgctgtg ctaaagcccg ttctgccaac
    37381 ggagcagaat caacacagac tttgaattgt attcttgtcc ttcgggctgc cgcctgaacc
    37441 gccgtacagc cggaattgcc aataaagtca gccaattcaa acagccgctt tttgatgaat
    37501 ggatgatagt cctcatacag cgcaaagcgg gcgatccgga cagaaggatt tctaaccagc
    37561 tcctctggtt tagggcggta attgagggcg acgcaaatga cagactttgc ctgcggcagc
    37621 agttggccgg ggtcgaaccg cttttgaata ttgcggtgca gatacgccat cttggcggca
    37681 ttgccgccct ctatccatcg ccggaagtac tctgcctgag cagactctat cggctcggcc
    37741 gtcgtaatcc ccactgcatc aaagccaacg tcaagggcct tttgtttgat atctgcttcg
    37801 atagtcatag cgctatctta aattgggcaa tccattatta caaggtcaga cttgacagaa
    37861 cagggaaaca gacataccat aacctttttg aaattcagta aaaggatgca gtatgtcgga
    37921 taaagatggc aaaaaaggat ataaagatac actgaatctt ccgcaaacca gttttgcgat
    37981 gaaggccaac ctgacccagc gcgagccgca gcagcgaaaa ctgtgggaaa aggaagatat
    38041 ttacggcaaa atcatggccg cccggaaaga ggccccgctg tacattctgc acgacggccc
    38101 cccgtatgcc aatggtgata ttcatatggg ccatgtcatc aataaagtgc tgaaggactt
    38161 tgttgttaaa tataaaacaa tgacgggctt ttgtgccccc tttgttcccg gctgggactg
    38221 ccacggcctg cctattgagg ttaaggtgat ggccgaactg ggcgaaaagg ccaagcagat
    38281 gtccaagccc gaaatacgca gggagtgcca taaatacgca tcgaaatatg tcaaactcca
    38341 aacgaaacag tttcagtctt tgggtgtctt cggcgacttt gaacatccgt acctgacgct
    38401 caagccgcag tacgagcagg ggattctgga aatctttgcc gaattggtcg gcaatggact
    38461 ggtctataag cagctcaagc ccattcattg gtctgtgggt tgtgaaacgg ccttggccga
    38521 tgccgagctg gaatacaagg atattacatc ttcgagcatt tatgttaatt ttccgatgga
    38581 tgccgcctcg gcgcaaaagg ttcggcagat gggtctggcg cctgcagaca gcaaggtctg
    38641 ctttatgata tggacaacga ccccgtggac cttagcggcc aatttggctg ttgcactgca
    38701 tccatggcat gattatgtgg gtttgacgta taacagaaac ggcacgacat ttacctcgat
    38761 tgtctgtgcg gatcgtctgg aagcagttat taaggccgcc ggttgtccgg aagggcagta
    38821 ttctgtcagc cagcccgtca aaggcagcgc cttcgaaaaa ctgcggtatc tgcatcccta
    38881 tattgagaaa aatccgaccg ataaggacgc ttattttgct attaatgcgg agtttgttac
    38941 caccgatgac ggcaccggca ttgtgcacat tgcacccggg catggagccg aagactatgt
    39001 ggccggaatg cagtacgggc tggctgttta ttcgccggtt cgtgatgatg gtacgtatga
    39061 tgatacggtg cctcaatggc tgcgcggcaa aaaagtactt gaggttgata ccgaagtaaa
    39121 tgcctacctc aggcagttgg ggctgctggt gcatgaagac agcattctgc acagctaccc
    39181 gcactgctgg cgaagcaaaa tgccggtaat tttacgtgcc accgagcaat ggtttatcag
    39241 tgtggataaa gatgtggaaa agttcggcgg taaaagtctt cgcaatctgg cactggaaca
    39301 gataaaagat gtaacctggg tgccgagctg ggggcaaaag cggattcagg gaatgctcga
    39361 atcccgccct gattggtgca tcagccgcca gcgggcttgg gggctgccga taccggcgtt
    39421 tgtcaacagc agagggcagt cgctgttgac caaggagtcg gtcttggctg ttgcgcggca
    39481 tatcggcaaa aagggttccg acagctggtt taccgattcc cccaaagaga ttttaggcag
    39541 cgattttgtt ctaccggacg gatttgactg ggatgacctg cgcaaggaag aaaatatatt
    39601 tgatgtatgg tttgagtccg gctgcagctg gcacagtgtc atacggatgg ccggccagcc
    39661 tgtaccggtg gatttatact tggaaggttc tgaccagcat cgcggctggt ttcagctgtc
    39721 gcttctgccg gccttggggg caaccggcag gccgccgttc aaaacggtgc tgacgcatgg
    39781 ttttactgtt gatgaaaagg gaatgaagca gtccaagtcg gcaggcaact atgtcagtgc
    39841 gctggaggaa atagaaaaat acggggctga tgttctccgg ttgtgggttg ccagcgtcaa
    39901 ttatcaggaa gatatgcgct gcagcgatga gctgattggc cgtctgcagg aggcctatcg
    39961 caaaatacgc aataccctgc gataccttct gagcaacatc aatgacttcg tgccggccga
    40021 aatggctgtg gactataagg atatgcaggt ggttgaccag tgggcgatgg aaaagcttca
    40081 ggaactgatt gctcaggtgc acgaagcgta cgaaagcttt atgttccatc gcgtctatgg
    40141 attgatttac aatttctgca cagtcgagat gagcagtatc tatatggatg tgcttaaaga
    40201 ccggatgtac tgcgatggag ccgccagccg cagccgccgc agcggacaga ctgcgatgta
    40261 tcgcatactc gatgcgctgg tgcggatgct ggcgcctatc cttgtccaca ctgccgagga
    40321 ggcgtgggct gcgatcccct ataaaagtca gcccgttgac agcgttcatt tggcgctgat
    40381 gccgcagccg gatgcgtcca tcaatccctc tgccaatgcc ggccgatggg gaaaaattat
    40441 ggctctgcgg gatgacgtgc tcaaagtgct ggaaccgctt cgcaaaaatc agcttatcgg
    40501 cagcaatcag gaagcggcgg tacagattga aacaaatgat gaggaactgc tgacgatcct
    40561 caatcagata ggcattgaga cttttgctgc tctgtgcatt gtcagtcagg tttctgcggc
    40621 caagggagcg gctcttaaga ttacagcgtc taagagtccg taccccaagt gtcagcgctg
    40681 ctggaactac tggcctactg tcggccagaa tgccgactac cctgacctgt gtgaccggtg
    40741 tatcaaggtg actgccggat gctgaaagga aagctgctct ggactgataa cagggcaggt
    40801 ttattctcca aagaataagg gcagatttgc atcagcgagc ttttctgaac gccttagata
    40861 ctgggggttg gaaattgaca tatcagggtg tataatagcg caggtcaacc gagcagaata
    40921 gatagggatt ggataagcga tgcgaaatat agcggcaggg atgcagaaaa aggtctggca
    40981 gatacgtccg gccagccgtg atgcggctaa attggcccag tcgctgaata tatcgccgct
    41041 ggtcgcgcaa attcttatta accgccagct tgaccagacc gaaccagccc gtgctttttt
    41101 atctcccaaa ctgagcgact tgattgcgcc ggaacagatg ttcggcattt ccgaagcggc
    41161 ggcgtgcatt cgtcaggcga ttgaaagccg caaaaaaata tccatttacg gcgattatga
    41221 tgtggatggc atcaccagtg tggcaattct gtggcatctg ctgcagatgc tgggggcgca
    41281 ggtggattat tatattcccc atcgtatcga tgagggctat ggtctcaaca gcgacgccgt
    41341 gcgccgactg gccgccggcg gcacagagct tctgataacg gttgattgcg ggattaccgc
    41401 tgtcgaaccg gtgcggctgg ctcgggagat gggcatccaa acccttataa ccgatcatca
    41461 tcagccgggg gcagttctgc ctgatgcggc ggctatcgtt catccgcggc tggatcccgc
    41521 ctacggcaat ccgcactcgg ccggtgcgat ggtggcattt aagctggctt gggcagtggc
    41581 taatgcctac aaaaaaggag ttcaattatc agccccgctg cgtcaatatc tgctcaatgc
    41641 caccacgctg gccggcatcg gcacgattgc cgatgtggtg gaccttcggg gtgaaaaccg
    41701 tgtgctggcc ggctatggat tgaaggcgct ggaggcctcc aatcttgtcg ggattcggac
    41761 gctggtgcag acagccgagc tgtatgccgc tgatgtcaac agctatgctg tagcattccg
    41821 tctggccccg atgctcaatg ccgccggacg aatgggacat gcccgtctgg cagtcgagct
    41881 gctgacgaca gaaagcgaag tccgagcaat ccagattgca cagtacctca aagaccagaa
    41941 ccgtctccgc cagaaatatc aattggatat cttcaagcag gcccgccagc ggattgtgca
    42001 gatggggctt catcatccgg accgaaaaac cattgtgctg gctgatgaga actggcacac
    42061 tggtgttatc ggcatcgttg ctgcccggat tgtagatgat ttttatcggc cgacgatttt
    42121 gataaacacc ggcgagggaa ttgggcaggg ttccggccgc tctatcgagg gctttaattt
    42181 gcatgcgcat ctggaggcct gttcggacta tctgataagt ttcggcggcc acgagatggc
    42241 ggctggcttg agaatcgaac cgtcgcgcat agcggcgttt gcagaggcat ttgaggcatc
    42301 cgcagaacag gcattcaggg aaaagatgcc cgcgtcctgt ttgacgattg atgccgaagt
    42361 gcgtatagcc gattttaccg aacggctgat gaaagagctc aactacctgg agccgttcgg
    42421 acagggcaac cccaaacccc tctttgccgt tcgcggcgtc aagctgattg ctcccccgcg
    42481 caaggtcggc gcccgcagcg accacctcca gctttcgatt gccgatgcca gcggagctgt
    42541 ccgctgta
//
LOCUS       DAOTBO010000016        38323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_858_length_38323_cov_5.454766, whole genome shotgun sequence.
ACCESSION   DAOTBO010000016 DAOTBO010000000
VERSION     DAOTBO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 38323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38323
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_38323_cov_5.454766"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLE88_03575"
     CDS             complement(<1..553)
                     /locus_tag="PLE88_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOM75581.1"
                     /translation="MKQRVVITGMGIVCPLGNDVAAMWNGLMTGQSGAGRTTLFDASA
                     FPTTFDAEVKNYDPARFVKYMHLHKNGNRGSLFVLGACVQACRQAGIDVETDAPADGI
                     DRTRLGIYLGAGEGPAVADAFFNSIAESWDSQAHRFDWPRWTHAFASRTTPALELEQE
                     PNMPSAHLALLTGARGITRNCLTA"
     gene            complement(554..1876)
                     /locus_tag="PLE88_03580"
     CDS             complement(554..1876)
                     /locus_tag="PLE88_03580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOM75582.1"
                     /translation="MNKRRVVVTGIGAVTPLGLSSSDLWSGLLEGRCGIRKIRAFDAS
                     AFPCQLAGEVAEYNIRDYVPKSYRKATKLMSRDIELSVIAADEAIRNSGLITKAAETE
                     SRSVPPERFAISFGAGLISCDMQEIAPCIAMSLTDGRFDIRKWGKDGMQALTPLWLLK
                     YLPNMLPCHIGIIHDIQGPSNTITCGETAGHIAVAEAAEMIARGDADAALAGGCEAKV
                     NPIVMLRQCLLKRSAENANDTPEQACRPFDADAGGSVFGEAGGVLVLEELQAAVCRKA
                     PIAAELVGAASSNNLNADFIHLESDGKGLQIAIRKALEEAHIRPEQIDLIIPTGTGIA
                     ADDRAEAAALEAVFGSALQTINVWPIKSMVSHTGAAAGVVDLIAAVLAIQHGTIGPAV
                     NFSRPADGCRLKIHTKQTTQNIRFALCCGYSFGGQTAAVVIKKYEDSL"
     gene            complement(1873..2358)
                     /locus_tag="PLE88_03585"
     CDS             complement(1873..2358)
                     /locus_tag="PLE88_03585"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOM75583.1"
                     /translation="MRWIWIDKFLEFKSGQYAVALKNVTLAEEHLHDHFPGFPIMPEC
                     LMIEAMAQTGGILVGEARNFAEKVILAKIKKAVFYHYVCPGDTITLHARIESIAPEAA
                     SIEGEIKRGDQRIAQISLMFSHIDKNLAGKQFPEENFVFTDMFHSLLTGFVERPTEPA
                     L"
     gene            complement(2453..2845)
                     /locus_tag="PLE88_03590"
     CDS             complement(2453..2845)
                     /locus_tag="PLE88_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOM75584.1"
                     /translation="MAMSRDEIFEKVKDVLVDALGLDDDDVTEDATLMGDLGAESIDF
                     LDIVFRLEKAFNIKVPREELFPAEAVMNNPEYVSGGKVTAKGLAELRARMPHTDLSNF
                     EKDPDVNKIADLFTVGAVVNFIESKISA"
     gene            complement(2908..3405)
                     /locus_tag="PLE88_03595"
     CDS             complement(2908..3405)
                     /locus_tag="PLE88_03595"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOM75585.1"
                     /translation="MGFLTKIPPMKFVLIDKITALESGQRLTAVKSVSLAEEYLADHF
                     PTFPVLPGVLLLEGLIESASWLVREYQNFSNSMILLESAANVKYKSFAAPGMQIHYTV
                     TAAAIEQNTSSFKGVGTCGDESIVEAKLTLRHFNLADENPRLARTDTILIENLKQRWK
                     LLYNG"
     gene            3680..4597
                     /locus_tag="PLE88_03600"
     CDS             3680..4597
                     /locus_tag="PLE88_03600"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75586.1"
                     /translation="MAQSRRFSLKLAVIVLLAAGIVTDKLSATAHETELSTRTLRSMA
                     RAYMAFGKYDKAHILASQALQQARSGKTDAGELALCLIDMGTVCSYEGLLNEARQRFE
                     EGAELQKQALFESHPYVAHTLRMLSAVCRRQGDLEQAQSCLSQAIEIMLRHCGLQDRE
                     MAPFILESANLQFAQGQMEQAEGNYRSALDMYEQYYGSEHLMTADVLEQMSKFWLACN
                     NFTQAQETMSQSLAIKSRIFGRYHPMLIDGWLTMARLSRMQGQMERCEYYLAKTEEAA
                     ALTRNAVTLAQVYEQINRIRKADLVAAAF"
     gene            complement(4607..5236)
                     /locus_tag="PLE88_03605"
     CDS             complement(4607..5236)
                     /locus_tag="PLE88_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75587.1"
                     /translation="MNARLNSGKLGLCILAGIVLTGCPIQKRLPIPQQTFLLEVQRPQ
                     EEKPAALPVCLRISSCTPASPYAGTPFIYRTGPTVYEKDAYNAFLVSPDEQITEILNQ
                     WYLSSGLFSCPPKADTLRMEPHLNILHTDFRDKNKPAAVVQMNFRLTRTDKTCSCPIV
                     LLNKTYSAEIPLLQTPAASDIVSGLSTALTSILQELERDVVQSIASTSL"
     gene            complement(5233..6180)
                     /locus_tag="PLE88_03610"
     CDS             complement(5233..6180)
                     /locus_tag="PLE88_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOM75588.1"
                     /translation="MKQNSTYFKIGIFILGCTAILLGGLAWLSSSAFRGKPVLVETYL
                     NESVQGLNIGSAVTHRGVKIGRVKTITLVALEYALKPDTPEYNAFHSYVAVIMEIDSR
                     AFLGVDNNRLLVESLLDSKIKLGLRFKLSFQGITGLAYMEADYFDPQRSVPPDVPWKP
                     KYIYVPSTASLFTSFTQAVERIYQRLEKMPIEGVLEKMQNTLSSMDQAVQEAEVGKIS
                     QSVTALAEDLRQTNRRLQDFLESAKSLPADFDSAILQLNSTLSHIEQLIDRNESDVEK
                     LLINLNIVTQHLKQLAEKLNTDPAQFLLSKPRPPSEYIK"
     gene            complement(6199..6960)
                     /locus_tag="PLE88_03615"
     CDS             complement(6199..6960)
                     /locus_tag="PLE88_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM75589.1"
                     /translation="MNEPIIQVENLTAGYEDTVILERVSFEVYRGEIFVILGGSGCGK
                     STLLRHLIGLQTPMAGNIRILGQDITAAAGSQKEQILSTFGVSFQGGALLGSMTAIEN
                     VMLPLSEHTNLPTGAIEWIAHQKLELVGLGGYADYKPAQLSGGMQKRAALARAMALDP
                     QILFLDEPSAGLDPITAAQLDELIIQLSQSMGITFVIVTHELQSIFALASRAIMLEKN
                     AKGIIAQGHPNDLKNDKTNPAVWRFFNRMPPSLPV"
     gene            complement(6965..8107)
                     /locus_tag="PLE88_03620"
     CDS             complement(6965..8107)
                     /locus_tag="PLE88_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM75590.1"
                     /translation="MAEPSADKSFGTTFSVKRSKTGEVHLELAGSLTYAFVGSLWNPC
                     IHAVRHSNPQKLIIELDKMKDYDSAGIGLLTFLQSLGRKKGYSVEIAHLPTELAALLE
                     RYCQTGEKPSAPPAGLGAVASLGKTAAEILADIHSLIAFFAQVLVTGLRYAVHPLRFR
                     WNDFFVMTELAGFRALGIVSLLGFLFGLIIAFSSAMPLRQFGVEIYVADLVAIALARV
                     LGPFITAIIVAGRTGSAYAAEIGTMKINNEIDALEVMNLDPVGFLVLPRVLAAILATP
                     LLTVIANLWGLAGSGLVILSLGYPLSSYLQHVESMLSAADVLVGIVKAVVFGGLVGMV
                     GCLRGLQTQLGAGAVGIATTRAVVTAIILLVITEGIFAVLLHYLGI"
     gene            8394..8843
                     /locus_tag="PLE88_03625"
     CDS             8394..8843
                     /locus_tag="PLE88_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75591.1"
                     /translation="MKRAIFLLWTFICLLSVSEGKICYLVLSPTQPTSSDVIELFAGG
                     TTECNFANIDFMRFNAEGNVLQWDIYYSMGLLTIVDAWDASKSIGTFSPGSYELVVNV
                     YATQQPYGSYYLDDRGYLTFNVVPEPATLLLAASGGLVFLFKPRKTR"
     gene            complement(8864..11311)
                     /locus_tag="PLE88_03630"
     CDS             complement(8864..11311)
                     /locus_tag="PLE88_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOM75592.1"
                     /translation="MTESKRLKEVLDSGQFAVLAELTGGPGFNFGPIEKFLFDHQQNK
                     KPLPEGFCFAGIALPQNPGGVANIDPADVLAAVTGKNLLGQLDCIPHVSCKDSNSDGL
                     ISTLAGYRQQGIQSILALTGDKPASAKKVFELESIGLLSLIKRLNRQAMLAAKPGQWN
                     SVHQFYAGAAVSPYKYTEPSLMQQYYKMEKKIAAGAEFLITQVGWDWKKTLELTLYIK
                     DNRIKIPVIGNAYWLTTLTPAPRLMHDIKLAGCFVSNALLSKIQSESIDEHIERTAQQ
                     VAMFKSLGYAGVDIGGVHNYDIFLKILTRAAEIGTDWEKFKDNLFWPAENPFYLYDEA
                     GKPVKLSKTKKKPNERFFDFMHRAILDPQYRGFRCFKKTVKFFNADKEDTITYKLFNA
                     VEKAFKYCVFDCQECGDCYLPENFGYCTIGGCEKGLDNAPCGDATVEGYCGNNLRRLC
                     IGEKIYNSASAEPGGRDKLRRIINKPRIPALEHTSSLVNYLFGKDHTMKDPLIGIGES
                     IHASIPKTGQIMKELHNLGTDAYTRPSGPLNYIRALIEDQAAEGASYIAVNLDAFGEN
                     NPQQAVSMMIEYTRLVRQWGKGVPICVDSSNDDVLTAGLKEWYNTDQPVRPPLLNSIK
                     VYTADRMMPLKKTYDFAFIGLLVSEEKPTGPGGSHSIDELYALAHQLFDKAMQYGFKP
                     SEIFFDSTVFPIAIDMPMEPGVPGYTYRAFETIKKIKNDPKMRGCHFSLGISNCCRDL
                     PGRKIGICRAYVAKAMEYGLDAGIINVSHHYGQTPPDPELLKLIDAYAKLDGTIEKLN
                     EAMTLMGQFCASCRKPV"
     gene            complement(11748..12806)
                     /gene="thiE"
                     /locus_tag="PLE88_03635"
     CDS             complement(11748..12806)
                     /gene="thiE"
                     /locus_tag="PLE88_03635"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOM75593.1"
                     /translation="MNSILYRIIDANYNRAREALRVMEEYCRFGLNHSGLSGRAKQLR
                     HTLCATLSKLDTFALLCSRDTAGDVGRERKVEGQINRQTIEDCFTAASKRASEALRAL
                     AETAQTIDPEIAAVMERLRFEVYTLEKDAAMASYVKTKFEKVRLYVLINADPHTSDTD
                     VINLARICAENGADCLQLRAKNLTDARLLSLAEAFTATCRQNGTVSIINDRTDIAVLA
                     GADGVHLGQNEIPVPKARRLAGAPLIIGVSTHNTDELHKAIDNRCDYVGLGPAFASPT
                     KPHVPAAGLGYIRHALPLLEQAGIFHTAIGGITLDNLMELQQAGVKTIAVCQTIAGAD
                     EPAFICKALKNKLLNPCC"
     gene            complement(13466..13548)
                     /locus_tag="PLE88_03640"
     tRNA            complement(13466..13548)
                     /locus_tag="PLE88_03640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13513..13515),aa:Leu,seq:caa)
     gene            complement(13598..14704)
                     /gene="proB"
                     /locus_tag="PLE88_03645"
     CDS             complement(13598..14704)
                     /gene="proB"
                     /locus_tag="PLE88_03645"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOM75594.1"
                     /translation="MRNFKQAKRIVIKIGTSTLTTTDGVNSAYIRTLAAQVAYLRQLG
                     KQVILVSSGAIGMGAKRLKLTGPVTDMKMRQACAAIGQPLLMHEYHKAFGEAGMIVSQ
                     VLLTAEVLNHRKSYLNLRNAIEQLLHLGIVPIINENDCVSTAEIGTAFGDNDKLSALV
                     ASKIDADLLIMLTDIDALYDKDPRKYKEAKPVHFVEEITEPLIKAAGGRGSAHGTGGM
                     KTKLQAASIASAAGCRTVLAHGKEPDVIQKIIDGQIIGTLFAARQEKLSNRARWILHN
                     RPEGTIYIDEGAMAAIRKHKSLLPSGVKGVEGTFPTGAVVMINDAAKAITSLSSDELN
                     RVAGLHSTKIRDLLGPNRRDVVALPEDIVFVETG"
     gene            complement(14707..16005)
                     /locus_tag="PLE88_03650"
     CDS             complement(14707..16005)
                     /locus_tag="PLE88_03650"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOM75595.1"
                     /translation="MSIIETARLARNASIRLAAVESAQKNRALEAIRRAIADDAQAIF
                     AANQQDLRQAEQEGLSAPLLKRLKFDQNKIDEACAGIDALIRLEDPVGRTLSAIELDT
                     GLELYKVTCPIGVIGVIFESRPDALVQISCLCLKSGNAVLLKGGSEALRTNRILSDTI
                     MKAAAAAGLPNGWLGLLETRTDVAEMLHLDEYIDLIIPRGSNEFVRHIMNHTAIPVLG
                     HADGICHIYIDAQADIEMGVKITVDSKCQYAAVCNAAETLLVHRTIAAAFLPKLKEAM
                     DALQVELRGCPLTRQIIPVKEATEQDWKTEYLDYILSIKTVDSLTEAIDHINTYGSGH
                     TDAIVTQSRQAALTFMNGVDSASVFWNASTRFADGFRYGLGAEVGISTNKIHARGPVG
                     LEGLLIYKWHLAGSGHIVADYVGPNAKKYTHRKLNKDWIF"
     gene            16160..16230
                     /locus_tag="PLE88_03655"
     tRNA            16160..16230
                     /locus_tag="PLE88_03655"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16192..16194,aa:Gly,seq:tcc)
     gene            16266..17303
                     /locus_tag="PLE88_03660"
     CDS             16266..17303
                     /locus_tag="PLE88_03660"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOM75596.1"
                     /translation="MKCQQLTGIRQMRMVRLPVPALTRPNDVLLKIQYVGVCGSDVHY
                     YETGRIGSQIVAYPFTVGHECSATVEDFGAGVCGLEKGQPVVVEPAVSCGQCDQCRTG
                     RPHTCRALKFLGCPGQIEGCLCEYIVMPAECCFPTKGNLTLQQGVLCEPFAIGLYAVS
                     LSGLRKGQTAAVLGTGPIGLSCLVGLRHQGAARIYGSEKIPQRCSIAKTAGADWVGNP
                     DNEDIVRAILNQEPDGIDVVFECAGQQETLDQAVKILKPGGKLMLIGIPRQDTIHFSP
                     DQIRRKEIAILNVRRQNHCTQKAIDLLADGRVNLDFMITHTFSFDQTQEAFDLVSGYR
                     DGVVKALIRVS"
     gene            17519..18007
                     /locus_tag="PLE88_03665"
     CDS             17519..18007
                     /locus_tag="PLE88_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75597.1"
                     /translation="MHLDKSHSNNGCVIILARPADSLCSVLPAFLREYRFVCTVCAHI
                     YEVLIQLNASEIIRPAVLIVRPSMLTAESLAFLGRQYPKLHIIGWLEREERLSDLSFG
                     SNKGHIIPMTAGLKQLERMLKTLCRLGSVQDKQQPNEMRMLEHTRDVLTEQEVEALLG
                     AG"
     gene            18009..18992
                     /locus_tag="PLE88_03670"
     CDS             18009..18992
                     /locus_tag="PLE88_03670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOM75598.1"
                     /translation="MKKSQTTCMLIGSAQGVFSETPAGLRIYTSIFEGITAAQKDHWD
                     AIYAVLASIPHPRKQAIEALHSVCPQARIVLLVQMAEELQARELLDRFGWPSDWLNYC
                     ICPVSSRCLTAQTPASDDYRMTSDDKDRRIRELEKLVMQDDLTGLKNRRYLRHFLPTI
                     LQKADLSHSQITVLLFDLDDFKHYNDTYGHAVGDEVLRQTARLIRRCCRSQDVVARLG
                     GDEFAVVFWDASDAEHHSSRKTPEERRKSSQNHPREPVFMAERMRREISAAAFDQLGS
                     KGKGALTISGGLASYPGDGRTAEELLEKADQAMLEAKRCGKNRVLLVGQSA"
     gene            complement(18993..20288)
                     /locus_tag="PLE88_03675"
     CDS             complement(18993..20288)
                     /locus_tag="PLE88_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOM75599.1"
                     /translation="MPQEQHLNRRILLVDDEIRILDELKKVLAPSETTSQELQDLESR
                     LFGTSSKKSNRLKTYHVECCCQGDQAIEVVRNAIEIGKPFAVAFLDIRMPPGPDGVWT
                     AEQIRRLDPNVQIVMMTGYSDFDINEIARRVPPEDKLLYMQKPIHSQEILQFALALTA
                     KWQSDYLLHLQNQKLQEQNEALKEHDRLKSEFVMTVSHELRTPLTIFKNILSNAMAGV
                     MGKIPPKLQRNLEMADEAIARLTSIINDFLDISKLDVGRMKLYPEKLCVQNVIRDIVE
                     MIRFVTDAKNIQLELTLHSSDLYIYADYEKFARVINNLIENAVKFVPEQTGKITIRVE
                     DRGTRIGIAIEDNGPGVPDKDKEKIFDRFVQVEKHVGPGKHGTGLGLAICRELVQLHS
                     GRIWVEDNPGGGAVFRILLPKYDPLLAKESSAAVQPAAV"
     gene            complement(20292..22160)
                     /locus_tag="PLE88_03680"
     CDS             complement(20292..22160)
                     /locus_tag="PLE88_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75600.1"
                     /translation="MSLRERILVVLFFMLCCYAGLEYLVLKKILAPGFEKQERQQAQE
                     TLRRCTDAIGTQLSDLQKELSMQARSGRLYPLPTSSDNSLDTLMESLGLDFAVIYNLN
                     WKPIALAGAVTAEKADSVFFDADNTFINRNIPGYSSKALLYDARCPLLAAAVPIIRSP
                     VSQTIEGTLVGGRFLTRDYLESLRKNLQVQFNWEFLHPNQLSEFNKQIADHIDAGNPY
                     DFVPLGEQILQASTILYDCRRQPVVLIKTFQDRSISRQGIYTLYAALYIRLIAGFAAV
                     FLLTILLQNAVVKPINKLCWHIIDIGHSGSSKTALTLQRKDEIGILASEFNAMCRRLQ
                     EAQVKLMEKSYLSGLTEMSSGILHNVRNALSPITTRIERIKGQFHQIPLANLEQAQQE
                     LQCAVLEPQRRQDLMRFVELTIQNILMTLKEMLSGLDELSEQVIQIEDMLQMQRTFGR
                     NERPIQFMTPMQLLEKALEMVPEKFRKECRIQIHPQIKKLPPIPVEPVTFVQVLQNLL
                     INAAESLENEKPLYRKIHIVADVENSDGKNMLHWQIRDNGIGIEAENLRQIFERGAST
                     KRKGLSGIGLHWCANTITAMHGRIWAESPGSHHGATFHILIPVSTEEIPALVGKEI"
     gene            complement(22376..23065)
                     /locus_tag="PLE88_03685"
     CDS             complement(22376..23065)
                     /locus_tag="PLE88_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75601.1"
                     /translation="MKKTTSSAGKPMFGLRLFLLTVAAGAVGIVIGIFSLYRYTPGAY
                     QPPASPNSDQISPYLTHELAPDFLNRVQLDKSFELIIKQDGLNEIISQQDWPAELGEI
                     TFDKPMVIFANRAIYLMGTVNYRDLSSVATVQAQPVLNPDGRLRLNIQSIRLGLLPVT
                     TLVGTLVQQILDANQEYFSDQPQLAAVIEAIIRSEPFEPVFTIYNYTVRLSRFHIEEG
                     ILKLYLEPVRP"
     gene            23171..24112
                     /gene="pyrF"
                     /locus_tag="PLE88_03690"
     CDS             23171..24112
                     /gene="pyrF"
                     /locus_tag="PLE88_03690"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOM75602.1"
                     /translation="MANHFADRLADAVKQKNTPLVVGLDPVYGRLPEPIRKNRFMNDE
                     TDAGAAVDAIFEFCARTLKIAAPLVAAVKINIGYFERYLWEGIETYYALIAEAEALGL
                     EIIGDVKRGDVGHTALAYAEAHLQNPEFYDMEDIITPDAITINGFAGADSILPFADTA
                     DEQGKGVFVWVRSSNPGASTIQDFSDASGVKMYEKLAQIVGEIAGQSKRIGSSGYSNV
                     GMVVGGTSPDETAALRQRYPYCWFLVPGFGSQGATAPDCVRFCGQNGLGALINASRSI
                     IYAYENPHYKSQFGDNWEKCIEQAIIDAKMELARARF"
     gene            24124..25506
                     /gene="murD"
                     /locus_tag="PLE88_03695"
     CDS             24124..25506
                     /gene="murD"
                     /locus_tag="PLE88_03695"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOM75603.1"
                     /translation="MEPAFFKNKRIVVMGLGRFGGGLDSALFAAKAGAKVLVTDLAGE
                     SDLQDSISELRGLDIEYHLGGHKDDDFRHGDVLIVNPAVPPDNRFVQLAAQAGLLITS
                     QLELFFQMCPACIVGITGSNGKSTTTALTAHLLQAGCGQEGVGYKNVWLGGNIGHQPL
                     LDNLDNIDKADIVVLEISNFQLDQLARIQAAPHIALITNLTANHLDRHGTFESYCRTK
                     ENIFKYQRLDESRPAVSIFNAEDSITRAWYLKYLDQKGRRCLSFCADDVPNDAAAAFK
                     LPGRTNRSNLAAALAVVSCFNVPAKRVVEAAGTFEGLPNRCRLVAEVNGVRWYDDSKA
                     TTPVSTMAALNGIDEPKILIAGGYDKHISFAELGLCIAKRAKAVILMGQTAPKIAQAI
                     EAAGTTNCIIRYADSMEQAVRVSSDLAVPGDVVLMSPACASYDMFKNYIQRAEAFIQA
                     VKALEAPLRI"
     gene            25550..26461
                     /gene="menA"
                     /locus_tag="PLE88_03700"
     CDS             25550..26461
                     /gene="menA"
                     /locus_tag="PLE88_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOM75604.1"
                     /translation="MKLSLLGWRCWLRAARPFSWTASMTPVLLGAALVPYLQKSGRWL
                     LLPFIAAASLFMHAATNMVNEYFDYKKGVDTPDSFGGSRILVDGLLSPKQVLAGGLVL
                     YALTVCIGLIFIVFYGWPILLLGLIGLAGGFFYSALPIGYKYRGLGDPFVFILMGPLM
                     VIGSFFVLTGAYQTDVLLISLPIGCLVTAILSANNLRDIQHDTQAGIRTTASVLTHRW
                     ARAEYAGLVMLAYLITLTLMGLKILPLWSLLTFPTLLLAVRTVRAAWRSRLSRPEQIA
                     ALDVQTAQLHLLFGMQLIFSVLLGAFL"
     gene            26458..27072
                     /locus_tag="PLE88_03705"
     CDS             26458..27072
                     /locus_tag="PLE88_03705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOM75605.1"
                     /translation="MKALLVLLLAAQAGFFVLFSPWTKDIPFWPVMTVMTAVLGGGAL
                     ALDWQMLKAVFRFDCSAVILGVGSAIILYGLFWLGYWISSAVLPFAASQVQSVYAVRA
                     GQNPWIITGLLCLIIAPAEEIFWRGFVLHRLSRQFGFAAGLAAAAAVYALVHIWSFNL
                     MLSAAAGICGVYWALLFFLTQNLWSCIISHCLWDVMIFVLFPIQ"
     gene            27069..27779
                     /locus_tag="PLE88_03710"
     CDS             27069..27779
                     /locus_tag="PLE88_03710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOM75606.1"
                     /translation="MTNENAQFVFDAIAADYDRFNRLFSFGIDRWWRRKLVEMVRPHP
                     HQQILDLCCGTGEVIFSFLRHSLVRTVAGADISEAMIDFAIEKQIQYSTKPWMQNKSL
                     AWHVCDAAQTGLAAGSFDIITCAFGLRNISDCSAVLNEMRRLLKPKGKLGILEFALPS
                     HPLAGKVYDFYLNRLMPHLAKPVLGASDPVRYLADSIRRWHNTVDFAGQLDKAGFKLI
                     CKTPLTAGIAALWLAHSQ"
     gene            27870..29303
                     /locus_tag="PLE88_03715"
     CDS             27870..29303
                     /locus_tag="PLE88_03715"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOM75607.1"
                     /translation="MTFTQLLSLTQSSRLRVCADSRQVRPGDVFVAVRGTQADGHNFI
                     RQAAAQGAAYIVAQQPVAGIECILVEDSARALGLLAQASYDNPNSKLTNLAVTGTNGK
                     TTTSYLVRSVIERTGAKCGLIGTIEYSTGSSTTAAPLTTPDAVTLACAADEMVRSGAE
                     YMMIEASSHALSQKRLAGISFTAAAFTNLTGDHLDYHQTLDDYLEAKLSLFMDLPPHG
                     LAILNYESDEAHTIAEVIEPATRILWYGIDRDVDLIAHIHRMDAAGSTFSIIFNSISE
                     KVITPLPGRHNILNCLAAAGLCLAAGFTLSDIAEGLSILESVPGRLEPVDSEGVQRRK
                     IGVFVDYAHTDDALKNVLGTLKPLCKGRLIVLFGCGGDRDKTKRPRMARVAEQMADMI
                     IVTSDNPRTEDPVAIINDILSGFSDPHSSRLIVEPDRAKAIRRAIEEARPNDMVLLAG
                     KGHEKYQIVGTQKFHFSDAEEVLKVVG"
     gene            29330..30742
                     /gene="murF"
                     /locus_tag="PLE88_03720"
     CDS             29330..30742
                     /gene="murF"
                     /locus_tag="PLE88_03720"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOM75608.1"
                     /translation="MDPISLRQLIQGIEGLQLNPSVQPTADICGISIDSRTIEPQQAF
                     FAISGQNFDGHSYAAAAIEKGAACVIVEKIAMPPQECRVPMILTNSTVAALGRLAAWY
                     RQQLSAKVIGITGSVGKTTTRHILYQVLSARYKCRQAQKSFNNHIGLPLTILSAQKDD
                     DILLLEMGTNHPGEIAVLTNIANPDAAVITRVSPAHLEGFGSVEAILKEKASIALGVK
                     PDGTVYLNGDQPELATHIKTIFPGRVVTFGTTSDCDIAGTDLETEGCSGYLSIEGRRI
                     KVPLAGRANLMNVLTVWAICKDFNITLSDFQHAVEALTPIPMRLQIYSLGRLTILDDC
                     YNANPVSMANALSCLHSVASQGRRKVFLAGCMAELGAQSAQLHRELGAAATAEGVEIL
                     LAAGPFAKAVLEGAVQAGLPPERMAAFEKTDQLCDNLHKWIQADDIILIKGSRSAGME
                     KSIVRLRDLFEKQCISNARE"
     gene            30746..31885
                     /gene="mraY"
                     /locus_tag="PLE88_03725"
     CDS             30746..31885
                     /gene="mraY"
                     /locus_tag="PLE88_03725"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOM75609.1"
                     /translation="MIYHLLWWLRDIITHRLGFYAYQDVLFRAILAALTSLAIAVLLA
                     PRMIRWLMRMKVGDRPEFHHAALNELMRDRANTPTMGGIIILTAILAATGLWARLDNP
                     FIHKAVFILIWYGGIGAMDDWLKLTSKNRQHSRDGLKPWEKLVFQFGGAVLVAIFLYS
                     DFRQIEDARRLWLPFYKHGVPLAGWVFAIVTVLYISATSNAVNLTDGMDGLASGCLSI
                     MSTVLVILCYIASEYMSRSAQVTWASYLLLPHVPQSGELCIFYAAISGAMLGFLWYNC
                     HPAQVFMGDIGSLPLGAAMGYGAVVTRNEILLFVIGGVFMMELFSVVLQVAYFKYTGG
                     KRIFRCAPIHHHFHLAGWSEPQVVVRFWLLSVAFAVLALATLKLR"
     gene            31905..33191
                     /locus_tag="PLE88_03730"
     CDS             31905..33191
                     /locus_tag="PLE88_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOM75610.1"
                     /translation="MKLSLHYGQGRVELIIPQANLAGFIQPRHQATPSDGRQLLLQAI
                     EPCRRQLASRTQGRCVGILLPDGTRDLPHQEILPLLLEPLGRARKLLFFICTGTHPGN
                     TPANQEILRTIAACSMNLPARWEVIVHDCQDASFQSAGLTKRGTDVLYNSRLDEAEII
                     LCLSDIKHHYFAGYSNPIKNIVPGLCSFSVAEQNHRWTLDARSRAGFHPWHPCAGRQE
                     NPLACDMLEAAERIVRDRPFWALAVISSRKVIQWADLGLAQQVCARAFQRADEWNVHT
                     VEQTHYMVVSPGGLPNDVDLYIAQRALELTHSAAADGGHILFLAACPDGIGSPQTRDQ
                     FERPLTEPLESILNRRPSEYRLFSHKPYRQAMLIRRLSRLWMHTEIDSATVRAIHMEP
                     CTDPQQVIDDWLVQEPTAQILAVDGANKLLLECRTI"
     gene            complement(33210..33845)
                     /locus_tag="PLE88_03735"
     CDS             complement(33210..33845)
                     /locus_tag="PLE88_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75611.1"
                     /translation="MNIEQHPGTRIQKPMLKPVLLSIVILVSGIVIGSGLTWLALPRP
                     AMPELPPAPEFIRSRMVERMAEELHLSVEQQEQIKPIIAKHMNAIDALRAEAQPKIRQ
                     EIEAMNEEILQTLDETQKQRWQEQIKRMQEGFRRMHRRGPGEERRDRDEGRMGPGDDR
                     RDRDDQRMGPFDRQRPEMPGRPRRRMPDPADGNFLPEVQPPQLQPGTPPQP"
     gene            complement(33842..34102)
                     /locus_tag="PLE88_03740"
     CDS             complement(33842..34102)
                     /locus_tag="PLE88_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75612.1"
                     /translation="MKELTFSKLVSLARQECPPGVHAADEVIAALSCLMAQTADPYRA
                     YVWMGAFSAAAAACILIAVSQLGQSAADSVSEVMTFVSWVAQ"
     gene            complement(34102..34824)
                     /locus_tag="PLE88_03745"
     CDS             complement(34102..34824)
                     /locus_tag="PLE88_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM75613.1"
                     /translation="MIACSDQMFYTDKQMIEHLFEHLVIGALPGSNTAAIKPQIDGVQ
                     FASAASNKTSNILFSELDDICRSQKGDSEAYRRLIERYQSHISRLLWRFTRDKTFHEE
                     LVQETFVQAYLSLHTYKSQAPFEHWLTRIATRTGYRFWKQSNRHQHLSLDDGQWEQTA
                     AAEPDTLTPEQAADIVHRLLSQLPPRDRLVLTLRYLEQCDVEETARRTGWSQTLVKVQ
                     THRAKQKLKALLEKSHIQFEMD"
     gene            complement(35024..35896)
                     /locus_tag="PLE88_03750"
     CDS             complement(35024..35896)
                     /locus_tag="PLE88_03750"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOM75614.1"
                     /translation="MRLFISLGQSSVNELKFDQGPVYVGRQMGSQVFLPDKAVSRQHA
                     VFYTTKDGNWVIEDLGSSNKTYLNSHPIHKSELKHNDVVRIADFLIRVSLEPDEDQNR
                     RNELEDTLTVEHTAVARDLHTVERNFDALDAPPVKFAVKRIKQYHEAIELISKATKLD
                     MLYKNLMDLLFRQFIAKHVWMAFRKAPAGPMDIEGGRQITTEHIDRTDLAVPASLAEA
                     LAGNKYLLIHQLPRQIIARGIRSVMITPILTAHTCHGVIYVANSTDHPHYSLAEMDYL
                     ILLSIHVAYCIEKL"
     gene            36035..36119
                     /locus_tag="PLE88_03755"
     tRNA            36035..36119
                     /locus_tag="PLE88_03755"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36069..36071,aa:Ser,seq:tga)
     gene            36402..36572
                     /locus_tag="PLE88_03760"
     CDS             36402..36572
                     /locus_tag="PLE88_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75615.1"
                     /translation="MEQLQALDNRFLVYGREELIGISLQDIKHKVVDFPHGRSKQLCG
                     RILEQAQVIVAE"
     gene            complement(36689..36856)
                     /locus_tag="PLE88_03765"
     CDS             complement(36689..36856)
                     /locus_tag="PLE88_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75616.1"
                     /translation="MKKGAPLPALAFWGTIFARLFTVNVAIYDETLKAQWTFNIQFST
                     NNDIKSLLIGQ"
     gene            complement(37241..38254)
                     /locus_tag="PLE88_03770"
     CDS             complement(37241..38254)
                     /locus_tag="PLE88_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOM75617.1"
                     /translation="MLISLVKLFLSLFQSRKQLLLENALLRQQLIICQQSVKRPKLKP
                     QDRIVLVWLSRVFSKWKESLVIVKPETVIGWHRKGFRLYWRWKSRKAGRPAIDWPLIK
                     LIRKLQKENPLWSPQRIQGELAKLGFNVCDNTVLKYLRKPKPDADKRQRWRTFLKNHA
                     HCIAAIDFLVVRTLFFRAIYVFIAISHERRRILHWGISSKPHSQWAIQQLRETFAFDE
                     STKYVIRDNDAIFREEFKQHIKRFGLKDTPTAPHSPWQNPIAERVNGTLRQECLNHVI
                     ILNEKHLRSVLTDYIDNYYNVSRTHMSLNKDSPVPRPVQTYGKIVSKPVLGGLHHVYT
                     RVA"
BASE COUNT         9230 a         9180 c         9806 g        10107 t
ORIGIN      
        1 aagcggtcag gcaattgcgt gtgatcccgc gagccccggt cagcagcgcc agatgtgctg
       61 aaggcatatt cggttcctgc tccagctcca atgccggtgt tgttcttgat gcaaacgcgt
      121 gtgtccagcg gggccagtcg aaacgatggg cttggctgtc ccaactttct gcgatggagt
      181 tgaaaaaagc atcggcaacg gccggtcctt cgcctgcccc cagataaatt cccagacggg
      241 ttcggtcgat tccgtcagca ggggcatcgg tttctacgtc gatgccggcc tgcctgcagg
      301 cctgtacaca cgcacccagc acaaacaggg agccgcgatt gccgtttttg tgcaggtgca
      361 tatatttgac aaagcgtgca ggatcataat ttttaacttc ggcgtcaaac gtcgtgggaa
      421 atgctgaagc gtcgaaaagg gtggtgcggc ccgcccctga ctgaccggtc atcaggccgt
      481 tccacatcgc cgctacatcg tttcccagcg gacaaacaat acccattcct gtaatgacaa
      541 cgcgctgttt catttatagc gaatcctcat atttctttat cacaaccgcc gctgtctgac
      601 cgccgaaaga gtagccgcag cataatgcaa accggatatt ttgggttgtt tgttttgtat
      661 gaatcttcag ccggcagccg tcagcaggtc gggagaagtt cacagcaggc ccgatagtac
      721 catgctggat tgccaaaact gcagcaatca aatcgaccac tcccgcagca gctcctgtat
      781 gcgaaaccat gcttttaata ggccagacat tgattgtttg cagggccgag ccgaacaccg
      841 cttccagagc ggcggcctca gccctgtcgt cagcagcaat gccggtcccc gtcgggataa
      901 tcaggtcaat ctgctcgggt ctgatatgtg cttcctcgag ggctttgcgg attgcaattt
      961 gaagcccttt gccgtcggat tccaaatgga tgaaatctgc attcaaattg ttgcttgatg
     1021 cggctccaac cagctcagcg gcaatagggg ccttgcggca gacagccgcc tgcagctcct
     1081 cgagcaccag cacgccgccg gcttctccga aaacagagcc gcccgcatcg gcatcaaaag
     1141 gccggcaggc ctgttcgggt gtgtcgttgg cattttcagc agagcgcttc agcaggcatt
     1201 ggcgaagcat cacaatggga ttgaccttgg cttcgcagcc gccagcaagg gcggcatccg
     1261 cgtcaccgcg ggctatcatt tcagccgctt ctgcgacagc aatatgtcct gcggtttccc
     1321 cgcaggtgat ggtattgctg gggccttgta tgtcgtgaat gatgccgata tggcagggca
     1381 gcatattggg cagatacttc aacagccaca ggggagttaa tgcctgcatt ccatccttgc
     1441 cccatttgcg tatgtcaaac cggccgtctg tgagactcat cgcaatacag ggagcgattt
     1501 cctgcatatc acagctgatt agtccggcgc cgaagctgat agcaaaccgt tccggcggaa
     1561 cggaacgaga ttctgtttcc gcggctttgg taatcagccc gctgtttcgg atggcctcat
     1621 cggcggcaat gactgacagc tcgatatccc ggctcatcag tttggttgcc ttgcgatatg
     1681 atttagggac atagtcgcga atattgtatt ctgccacttc gccagccagc tggcagggaa
     1741 acgccgaagc atcaaatgca cggattttac ggatgccgca tcggccttcc agaagacccg
     1801 accataagtc agaacttgac agccccagag gggttactgc gccgatgcct gtaactacca
     1861 cgcggcgttt gttcataggg ctggctccgt cggacgttcg acaaaaccgg ttagaagaga
     1921 atgaaacata tcggtaaata caaaattctc ttccgggaac tgcttgccgg caaggttttt
     1981 gtcaatgtga ctgaacatta agctgatttg agcaatccgc tggtctccgc gtttgatttc
     2041 cccttcgatg ctggcggcct ccggagcgat ggattcaatc cgagcatgga gcgttatcgt
     2101 atcgccggga cagacataat ggtaaaagac ggccttttta atctttgcca aaatgacctt
     2161 ctcggcaaaa tttcgtgctt caccgacaag gataccgcct gtttgggcca ttgcttctat
     2221 catcaggcat tccggcatga tcggaaagcc cggaaaatgg tcgtgcaggt gctcttcggc
     2281 cagcgtgaca ttttttaagg ccacagcata ttggccgctt ttaaattcta aaaacttatc
     2341 aatccatatc caacgcatga tttttgattt tctattaaaa aaggacatcg gcagaaaaga
     2401 tatgctctct cctgccgcgg cagagaaaat tattgtgccg aggcggccaa tatcaggcgc
     2461 tgattttgct ctcaataaag tttacaaccg cacctactgt gaaaaggtcg gcaattttgt
     2521 tgacatccgg atctttttca aagttgctca aatcggtgtg aggcatacgg gcccgcagtt
     2581 ctgcaaggcc ttttgcggtg accttgccgc cgctgacata ttcgggattg ttcatcacgg
     2641 cttcagccgg gaaaagctct tcgcgcggaa ctttgatatt aaatgccttt tcaagacgaa
     2701 atacgatatc cagaaaatcg atgctttctg cacccaagtc gcccatcagc gtggcgtctt
     2761 cggtaacatc gtcatcgtcc agtccaagtg catctacaag cacatccttt actttttcaa
     2821 aaatttcatc gcggctcatt gccataataa attgcctcca ttacggtctt tatggaattc
     2881 attaaagaac tcattctata cgagattcta tccattatac agcaatttcc aacgctgctt
     2941 aagattttca atgagaatag tgtcggttcg ggccaatcgg ggattttcgt cggccaagtt
     3001 aaaatgccga agtgtcagct tggcctctac aatggattca tccccgcacg ttccaacgcc
     3061 cttaaaactg ctggtattct gctcgatggc tgcggctgtt acggtgtagt gaatctgcat
     3121 acccggcgcc gcgaaactct tgtattttac attggcagcg ctttccagca gaatcatact
     3181 attggaaaaa ttctgatatt cgcgcaccag ccatgaagcg gattctatca atccttccag
     3241 caaaaggaca cccggaagta ccggaaatgt cggaaagtgg tccgccaaat attcttccgc
     3301 cagcgatacg cttttgacag ctgttagacg ctgacccgat tccagtgctg taattttatc
     3361 aatcagtaca aatttcatag gagggatttt agtaagaaat cccataattt ccagtttttt
     3421 ttacgttttt tctctgatgt aattaaatta caaaatatag cgatattgtg ggtatgcatt
     3481 attcgggata atcgggcact tgggatttag tcctataaag tgctgtttca tatgcaaaac
     3541 gatttataaa aaatttcccc atcgatagaa attctggcca attctgctca agtcagccaa
     3601 tctttcagcc gtaaagacac aatgtatctt atgttttaaa tggactattt gatagacgtt
     3661 ctgttaagag ggtagaagaa tggcccaaag ccgccgtttt tctctaaaac ttgccgttat
     3721 tgttttgttg gctgcaggaa tagtaacaga taaactctct gcaacagccc acgaaacgga
     3781 actatccacg cggacacttc gctcgatggc acgggcctat atggcatttg ggaaatatga
     3841 caaagcccat attttggctt cacaggcgct gcagcaggcc cgttccggca aaaccgatgc
     3901 aggggagctg gccttatgcc tgattgatat gggaaccgta tgcagttatg aaggcttgct
     3961 caacgaagcg cggcagcggt ttgaggaggg agcggagctc cagaagcagg ccttatttga
     4021 gagtcatcct tatgttgccc ataccctccg catgcttagc gctgtctgca gacgtcaggg
     4081 cgatcttgaa caggcccaat cctgcctgtc gcaggcgatt gaaataatgc tccggcattg
     4141 cggcctgcag gacagagaaa tggcaccttt tatactggaa tcggctaatc tgcagtttgc
     4201 acaagggcag atggagcagg cggaaggcaa ttacagatcc gcattggata tgtatgaaca
     4261 gtactacggc tccgagcatc tgatgaccgc tgatgtgctg gagcagatgt caaaattctg
     4321 gcttgcctgc aacaatttca cccaagccca agagacgatg tcccagtcgc tggcgattaa
     4381 aagccgcatt ttcggtcggt atcatcccat gttgattgac ggctggctga caatggctcg
     4441 gctgtcccga atgcagggac agatggagcg atgcgaatac tatctggcca aaacagaaga
     4501 agcggcggct ttaacccgca atgcagttac tttggcacag gtatatgagc aaatcaatcg
     4561 aatccgcaaa gcagatttgg tcgctgcggc attctaaacg gcagcatcat aaagaagtac
     4621 tcgctatcga ttgcacaaca tctctttcaa gttcctgcag aatgctggtc aaggcagtac
     4681 ttaaaccgct tacaatatcg gacgctgcgg gagtttgtaa aaggggtatt tcggccgaat
     4741 aagtcttgtt cagcagtaca attgggcagg aacaggtttt atccgtccgg gtaagccgaa
     4801 agttcatttg cacgacagcg gctggcttgt ttttatcccg gaaatcagtg tgcaggatat
     4861 tcaagtgagg ttccatcctc aatgtgtctg cttttggcgg gcaggaaaat aagccgctgg
     4921 acaggtacca ttgattcaaa atctccgtga tttgctcatc ggggcttact aaaaaggcat
     4981 tgtaagcatc tttttcataa acagttggtc cagtccgata gataaatgga gtacccgcat
     5041 aaggcgaagc gggtgtacag gaactgatgc gaaggcagac gggcaaagcg gccggttttt
     5101 cttcctgcgg gcgctgaacc tccagcagaa aagtctgctg cggaataggc agacgctttt
     5161 gaatggggca gccggtcaga actatccctg ccagaatgca gagccccagt tttccgctgt
     5221 ttaatcttgc gttcatttga tgtactccga tggcggtctt ggttttgaca acagaaattg
     5281 cgccgggtct gtattgagtt tttctgcaag ctgtttgaga tgctgcgtca cgatattcag
     5341 attgataagc agcttttcta catccgattc attgcggtca atcagttgtt caatatgaga
     5401 cagagtcgag tttagctgga ggatggcact gtcgaaatcg gccggaaggc tctttgcaga
     5461 ttcaagaaaa tcctgcagcc ggcgattggt ttgacgcaaa tcttcagcca gcgcagtgac
     5521 agattgactg atttttccta cttcggcctc ttgaacggct tggtccatgc tgctcagcgt
     5581 attctgcatc ttctccaaaa caccttcgat aggcattttt tcaagccgct gatagattcg
     5641 ctctaccgcc tgtgtaaagc tggtaaacaa gcttgctgta gaaggaacat aaatgtattt
     5701 gggcttccac ggtacatctg gcggtacgct gcgctgggga tcaaaataat ctgcttccat
     5761 ataagccagc cccgtaatcc cctgaaagct gagcttgaat cgcaggccga gctttatttt
     5821 gctgtcaagc agcgactcaa ccaggagccg attattatcc acccctaaaa atgcgcggct
     5881 gtcaatttcc ataatcaccg ccacatagct gtgaaaggca ttatactcag gggtatcggg
     5941 tttaagcgca tactccagag caaccagcgt gattgtcttg actcggccga tttttacgcc
     6001 gcggtgcgtg acggcagaac cgatatttaa gccctgtacg gattcattga gataggtttc
     6061 caccagcacc ggctttccgc gaaaggcgct gctgctgagc cacgccagcc cgcccagcag
     6121 aatggccgtg catcccagaa taaaaatccc gattttgaag taagtgctgt tttgcttcat
     6181 tgcagtcttc cgatggtatt ataccggcaa agaaggcggc attcgattga aaaatcgcca
     6241 gactgccgga ttggttttat cgtttttcag gtcgttggga tggccttggg ctatgatgcc
     6301 tttggcattt ttttccagca taattgcccg actggccaga gcaaatatgc tttgcagctc
     6361 atgtgttaca atcacaaagg taatccccat gctctgcgac aattgaataa tcagttcatc
     6421 cagctgtgcc gccgtgatgg gatcaagccc tgccgacggc tcatccagaa acagaatttg
     6481 cgggtccagc gccatagccc ttgccagtgc agcgcgtttc tgcatcccgc cgctcagctg
     6541 ggccggcttg taatccgcat agccgcccag cccgaccaac tccagttttt gatgagcaat
     6601 ccattcgatg gcgccggtcg gcagattggt gtgctccgac aaaggcagca taacattttc
     6661 aatggctgtc atagaaccga gcagtgctcc gccctgaaag gataccccga aagtcgagag
     6721 gatctgttcc ttctgactgc ctgcagcggc tgtgatgtct tggccgagaa tccgaatgtt
     6781 tccggccatc ggggtctgca gtcctatcaa gtgccgcaac agggtacttt tgccgcatcc
     6841 ggaacctccc aaaatgacga aaatctcccc ccggtacacc tcaaacgaga ccctttcaag
     6901 gataacagtg tcctcatatc cggccgttag attctctact tggataatcg gttcgttcat
     6961 tggattagat tcccaaataa tggagcagaa cagcaaaaat cccttctgtt ataaccagaa
     7021 gaataatagc cgtaacaacc gcccgtgtgg tagctatgcc caccgcgcct gctcccagct
     7081 gggtttggag gccgcgaaga cagccgacca ttccaacaag tcccccaaag actactgctt
     7141 tgactatccc taccagtaca tctgctgccg acagcattga ctccacatgc tgaagatagg
     7201 acgacagcgg ataacctaaa gacaaaatga ccaatccgga acctgccaaa ccccataagt
     7261 ttgcaatcac agtcagcagg ggagtcgcca atatagccgc cagtacacgc ggcagcacca
     7321 gaaaacctac aggatccaga ttcatcactt ccagagcatc aatttcgttg ttgattttca
     7381 tggtgccgat ttctgccgca taggctgagc cggttcgccc ggccacaatg atggcggtaa
     7441 tgaaaggccc cagtacccga gccaatgcga tagccaccaa atcggccaca tagatttcaa
     7501 ccccaaactg acgcagcggc attgctgaac tgaaggcaat aatcaatccg aacaaaaaac
     7561 ccagcagcga tactatgccc aaagcgcgaa agccggccaa ttcagtcatc acaaaaaaat
     7621 cattccagcg aaaccgcagc ggatggacag cataacgaag tccggtcacc aaaacctgtg
     7681 caaagaaagc aataagcgag tggatatctg ccaatatttc ggctgctgtc ttgcccaacg
     7741 aggcgactgc tccaagaccg gcaggggggg ctgaaggttt ttcacctgtc tgacaatacc
     7801 gctccagcag agccgcaagt tccgtcggca gatgagcaat ttcaacactg tatccttttt
     7861 tgcggcccaa tgactgcaga aaggtcaaaa ggccaatgcc ggcactgtca tagtctttca
     7921 ttttgtcaag ttcgataata agtttttgag gattgctatg acggacggca tggatgcagg
     7981 gattccatag agaccccaca aaggcatatg tcagcgaacc ggccagttca aggtgaactt
     8041 ccccagtttt gctgcgcttt accgaaaaag ttgtgccgaa tgatttgtct gcagatggct
     8101 ctgccattta tcgtaagccc gataagatac tttacataat ctgtccaaaa acttgtaacg
     8161 gatgcggtaa tgcctgtcaa gagcattgtt tttaatgccg cagattatat atttttaaga
     8221 tctgaaagta ccgaaccagc aggcaaaaaa acaatctgcc aagtgtcttg ttaatttatg
     8281 tatcaagata tattttatcc tatatgacag attttccttg atttgtttct taatttatgc
     8341 taaactaata gttgaggaat gggggagttg gtgagattgg agagattgga aagatgaaaa
     8401 gagcaatatt ccttttgtgg acgtttattt gcctgctttc tgtttcagag ggcaagattt
     8461 gttacctcgt gttatctcca acccagccga cctcttccga tgtaatagaa ttatttgccg
     8521 gcggcacgac tgaatgcaat tttgctaata ttgattttat gcggtttaat gccgaaggca
     8581 atgttttaca gtgggatatt tattacagca tggggctgct gactatagtt gatgcttggg
     8641 atgcctccaa gtccatcggg acgttcagcc ccggttccta tgaacttgtt gtgaatgttt
     8701 atgccactca gcagccctat ggcagctatt atctggacga tagagggtat ttgacattca
     8761 atgttgtgcc ggaaccggcc acacttctgc ttgcagcttc aggcggtctt gtctttcttt
     8821 ttaagccgcg aaaaacacga taataatcca gcgttaattt tttctatacc ggttttctgc
     8881 aggaggcgca aaactgtccc attaatgtca ttgcttcgtt gagtttctca atagtgccat
     8941 ccagtttggc atatgcatca atgagcttca gcagctccgg atctggcggc gtctggccgt
     9001 aatggtgcga tacattgata attcctgcgt ccaatccata ttccattgct ttggccacat
     9061 aagcacggca gatgccgatt ttccggcccg gcaagtcccg gcagcagttg ctgattccaa
     9121 gtgaaaaatg gcatcctctc atcttgggat cattctttat tttcttaatt gtctcaaaag
     9181 cccgatatgt atagcccggc acccccggct ccattggcat atcaattgcg ataggaaata
     9241 cggtcgaatc aaagaagatt tccgatggct taaaaccata ctgcatcgct ttatcaaaaa
     9301 gttgatgagc cagagcatat aattcgtcaa tcgaatggga ccctcccgga ccggtcggtt
     9361 tttcttcgct gaccagcaat ccgataaagg caaagtcata agtcttttta agcggcatca
     9421 ttctatcagc ggtataaact tttatcgaat tgagcagcgg cggccgtacc ggctggtcgg
     9481 tattgtacca ttctttcagg ccggctgtca gtacatcatc gttgctgctg tcaacacaaa
     9541 tcggcactcc tttgccccat tgacgcacca gccgggtgta ttcgatcatc atactgacgg
     9601 cctgctgcgg attattttct ccgaatgcat ccagattgac cgctatatag gaagcgcctt
     9661 cggcagcttg gtcttcaatg agggcgcgaa tatagtttag cggcccgctg gggcgtgtgt
     9721 aggcatcggt gcctaaatta tgcagttctt tcataatctg acctgttttc gggatggatg
     9781 catgtatgga ttcaccaatg ccgattagcg ggtctttcat cgtatgatcc tttccaaaca
     9841 aataatttac cagcgaactg gtgtgttcca atgccggtat ccttggtttg ttaataatgc
     9901 gccgcaattt atcacgtccg cccggttctg ctgaagcaga attatatatt ttctcaccga
     9961 tacacagccg ccggagattg ttgccgcaat aaccttctac agtagcatcg ccgcaagggg
    10021 cgttgtccag gcctttttcg catccgccga tagtgcaata gccgaaattc tccggcagat
    10081 agcagtcgcc gcattcttga cagtcaaaaa cacaatattt aaatgccttt tcaaccgcat
    10141 taaacaactt ataagtgatt gtatcttctt tgtcggcgtt aaagaacttg acggtcttct
    10201 taaagcaccg aaatccccga tactgcgggt caagtattgc tcggtgcata aaatcaaaga
    10261 aacgttcatt tggttttttt ttggttttgg acagttttac cggcttgccc gcttcatcat
    10321 ataagtaaaa cggattttca gccggccaga agaggttgtc tttgaacttt tcccagtctg
    10381 ttcctatctc agcggcacga gtgagtattt tcaggaaaat atcgtaatta tgcacacctc
    10441 cgatatccac gccggcatag ccaagtgact tgaacattgc aacctgctgt gcagtacgct
    10501 caatatgctc atcaatggac tcagattgga ttttggacaa taatgcgtta cttacaaagc
    10561 agccggccag tttgatatcg tgcatcagcc gaggagctgg agtgagggtt gttagccagt
    10621 atgcattgcc gataacaggg atcttaatcc tgttgtcctt aatataaagt gtcaattcca
    10681 gcgttttttt ccagtcccag ccgacttgag taatgagaaa ttcagcgcct gcagcgatct
    10741 ttttttccat tttgtaatac tgctgcatca atgaaggttc cgtatattta tagggagaga
    10801 ctgcagcacc ggcataaaat tgatgcaccg agttccactg gccgggtttg gctgccagca
    10861 ttgcttgtct gttgagacgt tttataagag aaagcaggcc gatgctttca agttcaaaaa
    10921 cctttttggc agaagcgggt ttatctccgg tcagggcaag aattgattgg attccctgct
    10981 gacggtatcc tgccagcgtg ctgattagtc catcgctgtt gctgtctttg cagcttacgt
    11041 gcgggataca atcaagctgc cccagcaagt tttttcctgt aacagcagcc agaacatcag
    11101 cagggtctat gttggctacg ccgccgggat tttgcggcag tgcaatgccg gcaaaacaaa
    11161 agccctccgg caagggcttt ttattctgct ggtggtcgaa gaggaatttt tctatggggc
    11221 cgaaattaaa acctggacct cccgttagtt cagctaatac ggcgaactgt ccgctgtcaa
    11281 gtacttcctt tagtcgtttt gattctgtca tcgtcttgct ccataaatgt gttcaagcaa
    11341 tgatcaggta ttataaagga ttataaaagt gtgaatatat tgaacttccg tttttttaat
    11401 acaattttgc tgtttgattc agtggagcaa tcgtcaaaat acgcgaaagc tgagatgtgg
    11461 acaaattgtt aataatttgg aggattatac tggtaccgga tgcaatatgc cttgatttat
    11521 ttatgcttat tgctatctct cctgatgttt ttgaaatgat tattttgcaa gtattgtttt
    11581 tcttcttata aactttttct attaaataag ttacgaatag ccattaagaa cagttgaatt
    11641 acagggggac tggtcggtat aaaaatacaa atttgaaatg ccgaaaaagc caattatttt
    11701 tatgatatat ctgtttgagc agttggtgaa aaagctgtcg ataactttta acaacaagga
    11761 tttaagagtt tgttttttaa cgccttacaa ataaaggcag gctcatcagc cccagctatt
    11821 gtctgacaga cggcaatggt ttttactcct gcctgctgaa gctccattag attatctaat
    11881 gtaatcccgc cgatagctgt atgaaaaata ccggcttgct cgagcaaagg cagggcatgc
    11941 ctgatataac caagaccagc agccggtaca tgcggtttag tcgggctggc aaaggccggt
    12001 ccaagcccta catagtcgca tctattatcg attgccttat ggagttcatc tgtgttgtgt
    12061 gtactaacgc cgataattaa aggagctccg gcaagccgtc tggccttggg aacaggaatt
    12121 tcattttgcc ccaaatggac cccgtctgct ccggcaagta ccgcgatatc agtccggtca
    12181 ttgataatgc ttacagtgcc gttctgcctg caagttgctg taaacgcttc tgccaagctg
    12241 agtaagcggg catccgtgag gtttttggct cttagctgaa gacagtcggc cccattttcg
    12301 gcacagatgc gggcaaggtt gataacatct gtgtcagatg tgtgcggatc agcgttaatt
    12361 aaaacataca gccgtacttt ctcgaattta gttttcacgt atgatgccat cgcagcatct
    12421 ttctcgaggg tatatacctc aaaccgcagt ctttccatta cggctgcgat ttccggatct
    12481 atagtttgtg ccgtttcggc cagtgcccga agtgcttcgc tggcccgttt agacgcggcg
    12541 gtaaaacagt cttcaattgt ttggcggttt atttggcctt caactttccg ttctcttccc
    12601 acatctccgg cggtatccct gctgcataac agggcaaatg tatccaattt gcttaatgtt
    12661 gcgcataggg tatgccgaag ctgtttagcc cgtccgctta gtccggaatg gttcaatcca
    12721 aagcggcagt attcttccat gacccttaaa gcttctcggg cacggttata attcgcatct
    12781 atgattcgat aaagaataga gttcataccc ttcaattgta aggaatttat gcagaaaaat
    12841 acagtgcagt taaaaaaact gggctggtat tctgtaccag ccccgcgcat caccctcgtc
    12901 cgctttcgcg gtagatgtca tttagattta ttaaatacga agatgtgcaa gccctgtgcc
    12961 ataagttgaa ataggacaat aatgcatcag aaataaagaa taactgtatt ttttataaca
    13021 gtttaggaat tttagatttc ggttgtcaaa aaaatgactt ggtgattttt gcagggacgt
    13081 tgcgaaattg caacgctgta tacctatagt tattcttata ggttattttt tatagggcaa
    13141 aagttaaatt tgtaacagcg aattaggtat gtttcaaaaa atcaacataa atatcaacat
    13201 ttgtttcaaa aattaacaca tttgacactt tccatttatg cgtgcagacc gttaatgggc
    13261 aaaccggccc ggactcgacc aaacgcggct tggacagaag atctttgtaa tgaataaaag
    13321 ctgcataata aaatgcaaat tacgatgttg caggtcttga aatggtgcct attcgagcaa
    13381 tccccataaa ataatagagc aggataagaa atagtgcgga aatgagacag ataacaatac
    13441 aaactccggc tgtcggttcc tatactaccg gaggtgggac ttgaacccac accctgttgc
    13501 caggacggga ttttgaatcc cgcgcgtctg ccattccgcc actccggctt atgaaggcga
    13561 tactatagac atttcaatca tctgcgtcaa tcgtttttta tccagtttcg acgaaaacga
    13621 tgtcttcagg cagagctact acatcgcgcc tattcggacc gagcaaatcc cgtatctttg
    13681 tgctgtgcag tcctgctaca cggttcaatt catccgaact gagacttgtt atagctttgg
    13741 ccgcatcatt aatcattaca acggctccgg taggaaatgt gccttcaacg cctttaaccc
    13801 cgcttggcag caggcttttg tgtttccgta ttgctgccat tgccccttca tcaatataaa
    13861 tggttccttc cggccgatta tgaagtatcc agcgggcacg gttgcttaat ttttcttgac
    13921 gtgcggcaaa caacgtaccg atgatctgac cgtcaatgat tttttgaata acatccggct
    13981 cttttccatg tgccagcacg gttctgcagc cggcagctga agcaatcgaa gcggcctgaa
    14041 gttttgtttt cattccgcca gtgccgtgag cactgccccg gccgcctgcg gcctttatca
    14101 gcggctctgt aatttcctct acgaaatgga caggcttggc ttctttatac tttcgagggt
    14161 ctttatcata cagggcatct atgtccgtca gcataatcag taaatccgca tcaattttgc
    14221 tggcgaccaa cgcgctgagc ttgtcgttgt ctccaaaagc tgtaccgatt tctgcagtgg
    14281 acacgcagtc gttttcgttg ataattggaa caatacccag atgaagcagc tgttcaattg
    14341 catttcgaag attcaaatag cttttacgat ggttcaggac ctctgcggtc agcagtactt
    14401 gggaaacaat catgcctgct tcgccgaacg ccttgtgata ttcatgcatc agaaggggct
    14461 ggccgatggc ggcacaggcc tgccgcattt tcatatctgt tacagggcct gtcagcttaa
    14521 gccgtttagc ccccatccca attgcgccgc tggagacaag gatgacttgc ttgccaagct
    14581 ggcgtaaata agctacttgt gctgccaacg tgcggatata agcactatta acgccatcgg
    14641 tagtcgtcag tgtgctggtg ccgattttga ttacaatccg tttggcctgt ttgaagtttc
    14701 gcatagtcag aaaatccagt ctttattcag tttccggtgg gtgtacttct tggcgttcgg
    14761 cccgacatag tcggcaacaa tatgaccgct gccggccaga tgccatttgt aaattagcag
    14821 tccctctaat ccgacgggac cgcgggcatg gattttgttg gtactaatgc ccacttccgc
    14881 ccccaagcca tagcggaaac cgtcagcaaa gcgggtgctg gcgttccaaa acacgctggc
    14941 cgagtccacg ccgttcataa acgtcaaagc cgcctgcctg ctttgggtta caatcgcgtc
    15001 cgtatgtccg gagccgtagg tattgatatg gtcaatcgct tccgtcagcg aatccactgt
    15061 tttaatagag agaatataat cgagatattc tgttttccag tcttgttccg ttgcttcctt
    15121 aacgggtata atttgccgtg tcagcgggca gccgcgcagc tcgacctgca acgcatccat
    15181 cgcctctttg agcttcggca gaaatgcggc cgcaatagtg cgatgcacca gcagggtttc
    15241 agcagcattg catacggcgg catactggca tttggaatct acggtaattt ttacccccat
    15301 ttctatatct gcttgggcgt caatataaat atggcaaatc ccgtctgcat gccctagaac
    15361 agggattgcc gtatggttca taatatgccg gacaaattca ttgctcccgc gaggaataat
    15421 caaatcgatg tattcatcca gatgcagcat ttcggcgaca tctgttcgcg tttcaagaag
    15481 ccccagccac ccgttgggca gaccggcggc ggctgcggcc ttcatgatgg tgtcagacaa
    15541 aatgcgattg gtccgcagcg cttcagaacc gcctttaagc agcacggcat tgccgctttt
    15601 aagacatagg cacgaaatct gcacaagagc atccgggcgg gattcgaaaa taactccgat
    15661 aaccccaatg ggacaggtaa ctttgtacaa ttccagaccc gtatcaagtt caatggcgct
    15721 gagagttctg ccgaccggat cttctaagcg gattagagca tcgatgccgg cgcatgcctc
    15781 gtcaatcttg ttctggtcaa atttcagccg cttcagcaga ggggcggaca ggccctcttg
    15841 ttcggcctgc cgtaggtcct gttggttggc ggcgaaaatg gcctgtgcgt catctgcaat
    15901 agcccggcgg atggcctcca gtgctcgatt tttctgtgcc gactcaactg cggccagccg
    15961 tattgaggca ttccgtgcca gacgggcggt ttctatgata gacatataat caatttcctg
    16021 ctcacaaact catttattat cccgaaaccg ttactatagc cgaaatgccc cccaaaagca
    16081 atgtttggcc attaaccatg aaaaaatcct aagaagccct tgcgtttgaa tggaaaggga
    16141 attataatcc ggctgttttg cgggcgtagc ttaatggtaa agccccagcc ttccaagctg
    16201 gtcatgacgg ttcgattccg ttcgcccgct ttatgaacaa taaaccgctt ggagcagccc
    16261 cctatatgaa atgtcagcag cttaccggta tccgccagat gcggatggtt cgacttcctg
    16321 tgccggcatt gacgcgtccc aatgatgttt tgttgaaaat ccaatatgtt ggagtgtgcg
    16381 gcagcgatgt tcactattat gaaacaggac gcatcggcag ccagatagtt gcatatccct
    16441 ttacggttgg gcatgaatgc tcggcgacgg tggaggattt cggtgcaggg gtctgtggac
    16501 ttgaaaaagg tcagccggtt gtagtagagc cggctgtttc atgcgggcaa tgcgaccagt
    16561 gccggaccgg ccggccgcat acctgccggg cactgaaatt tctgggctgt cccggtcaga
    16621 tagaggggtg tttatgtgaa tatattgtga tgcctgctga atgttgtttc ccaacaaaag
    16681 gcaatctgac gctccagcag ggggtactct gcgagccatt tgctataggg ctgtatgcgg
    16741 taagtttatc gggcttgcga aagggacaga cggcggcagt attaggaaca gggccgattg
    16801 gattaagctg tctggtagga ttgcggcatc agggtgctgc gaggatttat ggcagcgaaa
    16861 agattcccca gagatgttct atagccaaaa ccgctggtgc agattgggtc ggcaatccgg
    16921 acaatgaaga catagtaagg gcgatattaa atcaggagcc agacggaata gatgtagtat
    16981 ttgaatgtgc cggtcagcag gaaacgctgg atcaggccgt aaagattctc aagcccggcg
    17041 gtaaattgat gctcatcggg attccccgtc aagatacaat tcatttcagc cctgaccaga
    17101 ttcgacgaaa ggaaattgcc atccttaatg tccgacggca aaatcactgt acacagaagg
    17161 ccatcgattt gctggctgat gggcgtgtta atctggattt tatgattacc catactttta
    17221 gctttgacca aacacaggag gcttttgatt tggtttccgg ttatcgcgat ggtgtagtca
    17281 aggcattaat tcgcgtcagt tgattgtatt gcctgccccg acgaaatacc ctattttgct
    17341 catattaaat cccaatgtgc tattcctgca attgagtcta atcaatagta gtatattata
    17401 aggacatttg cttgcctgta tgaaggtttg agaaagccca aaagtcgatg aaaaatgcct
    17461 tttatagttg actttttgtc ccgaacaagt tatagtatta ttatcggaga tatgggtgat
    17521 gcatttggac aagagtcatt caaataacgg ctgtgtgatt atccttgctc gtccggcaga
    17581 ttcgctttgt tcagtgttgc cggcattcct gcgggagtat cggtttgtat gtactgtctg
    17641 cgcacatatc tatgaagtgc tcattcaact caatgcatct gaaataatcc ggccggcggt
    17701 tttaattgta aggccttcta tgctgacggc ggaatctctg gcttttctgg gtcggcagta
    17761 tccaaagctg catattatcg gctggctcga gcgtgaagaa agactgtcgg acctgtcatt
    17821 tggttcaaat aagggacaca ttataccaat gaccgctgga ttaaaacagc tggagcggat
    17881 gctaaagaca ctatgccgac tgggcagcgt tcaagataaa cagcagccta atgaaatgag
    17941 aatgctcgaa catacgcgcg atgttctgac cgaacaagag gttgaggcat tgctgggggc
    18001 tggataaagt gaagaaatca cagacaacgt gtatgctcat cggctcagcg caaggagttt
    18061 tttcagaaac tcccgctggt ttgcggattt atacttcgat ttttgaagga attacagccg
    18121 cacagaaaga ccactgggat gcgatttatg ccgttcttgc ttcgataccc catccgcgaa
    18181 aacaagccat tgaggcactc cacagcgttt gtccgcaggc acgtattgta cttctggtcc
    18241 agatggctga agaattgcag gcacgagaat tgttggaccg tttcggctgg ccgtcggatt
    18301 ggctgaatta ttgcatttgt cctgtcagca gccgctgttt aacagcccaa accccggctt
    18361 cggatgatta tcggatgacc agcgatgaca aggaccgccg aatacgagag ctggaaaagc
    18421 tggtgatgca ggatgatctg actggtctga aaaaccgacg ctatctgcgg cattttctgc
    18481 ctacaatttt acagaaggcg gatctgtcac acagtcaaat tacggttctc ctgttcgatt
    18541 tagatgattt caagcattac aatgacacat acgggcatgc ggttggcgat gaggtacttc
    18601 ggcagactgc ccggcttata cgccgctgct gccgcagtca ggatgtcgta gcacgactgg
    18661 gcggcgatga gtttgcagta gttttttggg atgcttctga tgcagaacat cacagcagcc
    18721 ggaagacccc cgaggagcgc cgaaagagca gtcaaaatca cccccgcgag ccggtgttta
    18781 tggctgaacg catgcgaaga gaaatcagtg cagccgcatt tgaccagctg ggttccaagg
    18841 gcaagggggc actgaccatc agcggagggc tggcatcgta tccgggcgac ggcagaaccg
    18901 ccgaagaact tcttgaaaaa gcggatcagg caatgcttga agccaagcgc tgcggcaaaa
    18961 acagggtttt gctggtaggc cagtcggctt gactatacgg ctgccggctg gacagcggca
    19021 gaagactctt ttgccagcag cggatcatat ttcggcagca gaatacggaa aacggccccg
    19081 ccgccgggat tgtcttccac ccaaatccgt ccgctgtgga gctgaactaa ctcacggcaa
    19141 atagccaatc caagtcctgt tccatgcttg cccggaccga cgtgtttttc gacctgaaca
    19201 aacctgtcga atatcttttc cttatcctta tcaggaacgc cggggccgtt atcttcaatt
    19261 gctatgccga tgcgtgtacc gcggtcttcc acgcggatgg tgattttccc tgtctgttcc
    19321 ggaacaaatt tgactgcgtt ttcaatcaga ttgtttataa cgcgtgcaaa tttttcgtaa
    19381 tcagcataaa tataaaggtc gcttgaatgt agtgttaatt ccagctggat atttttggca
    19441 tcggttacaa aacgaatcat ttcaacaatg tctcggatga cattctgaac gcagagcttt
    19501 tcaggataga gcttcatcct gccgacatcc aatttggaaa tatccagaaa atcattgatg
    19561 atgctggtca ggcgtgcgat ggcttcatcg gccatttcaa gattccgctg cagtttgggc
    19621 gggattttgc ccatcacgcc ggccattgca ttggacagaa tgttcttgaa aattgtcagc
    19681 ggtgttcgca attcatgtga aactgtcatt acaaattcac ttttgagccg gtcgtgttct
    19741 ttgagagctt cattctgttc ctgcagtttc tgattttgca ggtggagcag gtaatccgac
    19801 tgccatttgg ctgtcagcgc taaggcaaat tggagaatct cctgtgaatg aatcggcttc
    19861 tgcatataaa gcagcttgtc ttcagggggc acgcggcgtg caatttcgtt aatgtcaaaa
    19921 tccgagtagc ctgtcatcat cacaatctgt acattgggat caagccggcg tatctgttcc
    19981 gctgtccaga cgccgtccgg ccccggcggc attcggatgt ccaaaaaagc aacagcaaag
    20041 ggcttgccga tttcgatggc attgcggaca acttcaatcg cctgatcccc ctgacagcag
    20101 cattcgacat ggtaagtttt caggcgattg gattttttgc tgctggttcc aaacaggcgg
    20161 ctttcaaggt cctgcagttc ctgactggtt gtttcactgg gagccagcac ttttttaagc
    20221 tcatccagaa tgcggatttc atcatccacc agcagtatgc gtcgatttaa atgctgttct
    20281 tgtggcatta gctatatctc cttgcccaca agggcgggta tctcttctgt actgacagga
    20341 atcaggatat gaaacgtggc tccatgatga ctgccggggc tttcagccca aatgcggccg
    20401 tgcatagcgg tgatggtatt ggcacaccaa tgcaggccaa tgcccgaaag ccctttccgc
    20461 tttgtagaag ccccgcgttc aaaaatctgc cggagatttt ctgcttcaat accaataccg
    20521 ttgtcgcgaa tctgccagtg gagcatattt ttcccatcgc tgttttctac atcagctaca
    20581 atatggattt tgcggtacag aggtttttca ttttccagcg attcggctgc attaatcaga
    20641 agattctgga gaacctgaac aaaggttacc ggctcgacag gaatgggggg cagtttttta
    20701 atctgcgggt ggatctgaat gcggcattct ttacggaatt tttcgggaac catttccaat
    20761 gctttttcca aaagctgcat cggggtcata aactgaatcg gccgctcatt tcgtccaaaa
    20821 gtccgctgca tctgcagcat gtcctcaatc tgtatcacct gttcagacag ttcatcaaga
    20881 ccggacagca tttccttgag ggtcatcagg atattctgga ttgtcagctc cacaaatcgc
    20941 attaaatcct gccgtcgctg cggctcaagc acggcacact gcagctcctg ctgtgcctgt
    21001 tcaagattgg ccaaaggaat ctgatgaaac tgtcccttga tgcgttcaat gcgggtcgta
    21061 atcggactaa gggcattgcg tacattatgg agaatacccg atgacatctc cgtcaatcca
    21121 gacagatagg atttttccat cagtttgact tgggcttcct gcagccgccg gcacattgca
    21181 ttgaattccg atgccaatat accgatttca tctttccgct gcagcgtcag agcggttttg
    21241 ctgctgccgg aatgtccgat atcaatgata tgccagcaca gcttgttgat gggtttgacc
    21301 actgcattct gcagcaggat ggtcaggaga aacacagctg caaaacctgc tatcagccgt
    21361 atgtacaaag ccgcataaag agtgtagatt ccctgccggc tgatactgcg atcttgaaag
    21421 gtttttatca gcacaaccgg ctgccggcgg caatcataga gaattgtgga tgcctggaga
    21481 atctgctcgc ccagaggcac gaaatcatag ggattgccgg catcaatatg gtcagcaatc
    21541 tgcttattaa attcagatag ctgattcgga tgaaggaact cccagttgaa ttggacctga
    21601 agattttttc gaagcgattc aaggtaatcc ctcgtcagaa aacggcctcc aaccagcgtg
    21661 ccttctatcg tttggctgac aggcgaacgt ataattggta cagcggccgc caaaagagga
    21721 catctggcat catacagaag agctttactg gaatagccgg gaatatttcg gttgataaat
    21781 gtgttatccg catcgaaaaa cacagaatcc gccttttcag ccgttacagc gccggccaag
    21841 gcaatcggct tccagttgag attgtatata acagcaaaat caagtcccaa cgactccatc
    21901 agtgtatcca gcgaattatc ggatgaagtc ggcaggggat acaagcgtcc agaccgggcc
    21961 tgcatcgaaa gttccttttg cagatcgctc agctgtgttc caatggcgtc ggtgcagcgg
    22021 cgaagtgttt cctgtgcctg ctggcgttcc tgtttttcaa atcccggcgc cagaatcttt
    22081 tttaatacca agtactccaa gcccgcatag cagcagagca taaaaaacaa aactaccaaa
    22141 attcgttcac gcaatgacac ggctgttttt ccttgcagaa caggtatatc taaaacgatc
    22201 tgtctttgtg tctgtgctat cggaaaaata tagatgcaaa ttgcgggcag aatgcaaagg
    22261 ccgataataa cgctgccgaa atccaattgg caaaggaggg tgtgagatag gcattgatgg
    22321 aagaatcgct gcgtccgata aggatgcccc agaaaaaaca agcccgacga ggcgattaag
    22381 gcctgaccgg ttccagatag agcttcaata tcccctcttc gatatgaaaa cggctgagac
    22441 gtacagtgta attataaatg gtaaatactg gttcaaacgg ttcgcttcgg atgatggcct
    22501 caatgacagc tgccaactgg ggctggtcgg aaaaatactc ctgattggcg tccaatattt
    22561 gctggacaag cgtaccaacc aatgtagtta ccggcagcaa tcccagacga atcgattgaa
    22621 tattcagccg gagtctgccg tcgggattca gcactggctg tgcctgaacg gtagcaacgg
    22681 atgacaagtc tcgataatta accgtgccca ttagataaat ggcacgattg gcaaaaatga
    22741 ccatcggttt gtcaaaagtt atttctccca gctcggcagg ccaatcctgc tggctgatga
    22801 tttcattcag cccatcctgt tttataatca gttcgaagga tttgtctaac tgtacccgat
    22861 tgagaaaatc cggagctaat tcatgagtca gatacggact aatctggtct gaatttgggc
    22921 ttgctggcgg ctgataggcc cctggcgtat aacgataaag agaaaaaatg cctatcacga
    22981 tgccgactgc ccccgccgcc accgttaata gaaacagccg cagaccgaac attggtttgc
    23041 ctgccgaaga tgtcgttttt ttcatttccg gtttcctaaa aatgctgttg ctctgataaa
    23101 gcgttttgat tatagtaaca tacccgtaac tttacaggaa atttcagaat tatttgagaa
    23161 aggaaattca gtggcaaatc attttgcgga tcgtttagca gatgctgtca aacagaaaaa
    23221 caccccattg gtggtagggc tggatccggt ctatggccgt ctgccggaac ctatccggaa
    23281 aaatcgtttt atgaacgacg agacggatgc aggagcggcg gtagatgcaa tttttgagtt
    23341 ttgtgcccgt acacttaaaa tagcagcccc gctggttgcg gcggttaaaa ttaatattgg
    23401 ttattttgag cgctatctgt gggaaggcat tgaaacatat tatgccctta ttgcagaggc
    23461 cgaggcattg gggctggaaa ttatcggtga tgtcaaacgc ggcgatgtgg ggcatacggc
    23521 gctggcttat gcggaagcac atcttcaaaa tccggagttt tacgatatgg aagatattat
    23581 aactccggat gctatcacca taaatggatt tgccggagcc gacagcattt tgccctttgc
    23641 cgatacagct gatgagcagg gcaaaggtgt gtttgtctgg gttcgcagca gcaatccggg
    23701 cgccagcacc attcaggact tttccgatgc atcaggtgtt aaaatgtacg aaaagcttgc
    23761 ccagattgtc ggtgagattg ccggccaaag caagcgcatc ggctcaagcg gctacagcaa
    23821 tgtcgggatg gttgtcgggg gcaccagtcc ggatgaaaca gctgctttac gccagcgata
    23881 tccttattgc tggtttttgg tgcccggatt tggatcacag ggtgccacag cgcctgactg
    23941 tgttcgcttt tgcggacaaa acggccttgg ggctttgatt aatgcatcgc ggtcaattat
    24001 ctatgcctat gaaaatcccc actataaaag tcaatttgga gataattggg agaaatgcat
    24061 tgagcaggcc atcattgatg ccaaaatgga attggcaagg gctcgttttt aagcaaattg
    24121 ttgatggaac cggcattttt caaaaacaag cggatagttg ttatgggctt gggccgtttc
    24181 ggcggagggc tcgattcagc gctgtttgct gccaaggctg gggcgaaagt gctggtaaca
    24241 gatttagccg gcgaaagcga cttgcaggat tctatttctg aattaagagg tctggatata
    24301 gaataccatc tcggcggaca caaagatgat gattttcgcc atggcgatgt gctgattgtt
    24361 aatcctgctg tgccgccgga caataggttt gtgcagcttg cagcgcaggc cggcctgctg
    24421 attacctcgc aattagagct tttttttcag atgtgcccgg cctgtattgt cggtattaca
    24481 gggtccaatg ggaaaagcac cacaacggca cttacggctc atcttttgca ggccggatgc
    24541 ggacaagaag gtgttggcta caaaaatgtt tggctgggcg gcaatattgg gcatcagccc
    24601 cttctggata atctggacaa tatcgataag gcggacattg ttgtgctgga aatcagcaat
    24661 tttcagcttg accagctggc acgtattcag gcggctccgc atattgctct gattaccaac
    24721 ttaacggcca atcacctcga ccgtcatggc acattcgaat cttactgtcg gacaaaggaa
    24781 aatatcttca aatatcagcg gctggacgaa agccgaccgg cagtatcgat ttttaatgca
    24841 gaggacagca ttacccgtgc atggtatctg aaatatcttg accagaaagg gcggcggtgt
    24901 ctgtcctttt gtgcggatga tgtgccgaat gatgcagcgg cggcattcaa gctgcccggc
    24961 cgaacaaatc gttccaatct ggccgcagcg ctggcggtag tttcctgttt caatgtgcca
    25021 gcgaaacggg ttgtcgaggc agcgggaaca tttgaaggtt tgccgaaccg ctgccggctg
    25081 gtcgccgagg ttaatggtgt gcgctggtat gacgactcga aagccaccac acctgttagc
    25141 acaatggctg ctctcaatgg tattgatgaa cctaagattc ttattgccgg cggttatgac
    25201 aagcacatca gttttgctga attggggctg tgcattgcca agcgggcaaa ggccgttatt
    25261 ctgatggggc agacagcgcc gaagattgca caggcgatag aagcagccgg cacgaccaac
    25321 tgtatcatcc gctacgccga ctcgatggag caggccgtcc gtgtgtccag cgatctggcg
    25381 gtaccggggg atgtggtgct gatgagtcct gcctgtgcca gctatgatat gttcaaaaac
    25441 tacatccagc gggcggaggc attcatccaa gcagtaaaag cattggaagc accgttaagg
    25501 atttaacaac acccgatatc cgaaacaaaa tcgaattgag gaatcatctg tgaaattgtc
    25561 attacttggg tggcgctgct ggcttcgggc tgcgcggccg ttttcgtgga cggcttcgat
    25621 gacgccggtt ttactggggg cagctttggt tccgtatctg cagaagtccg gccgctggtt
    25681 gctgctgccg tttattgcag cggcatctct ttttatgcat gctgccacga atatggtcaa
    25741 tgagtatttc gattataaaa agggtgtaga tactcctgac agtttcggcg gcagccgcat
    25801 actggtggat ggcctgcttt cgcccaagca agtgcttgct ggcggtcttg tactgtatgc
    25861 cctgacagtc tgcatcggac tcatttttat tgtcttttac ggctggccga ttctgctgct
    25921 ggggctgatt ggattggccg gaggattttt ttactcggcg ctgccgatag gctataaata
    25981 ccgcggattg ggcgatccgt ttgtctttat tctgatgggg ccgctgatgg ttatcggcag
    26041 cttctttgtt ttgactggcg cataccagac agatgtcctg ctgatttcgc tgccgattgg
    26101 gtgtctagtg acggcgattc tttctgccaa taatctgcgg gatattcagc acgatacgca
    26161 agcaggcatc cgcacgacgg cctcggtgct gacacaccgc tgggcacggg cagaatatgc
    26221 cggtttggtg atgttggcgt atctgataac gctgacgctt atggggctaa agattctacc
    26281 gctctggtcg ctgctgacat ttccgacact gctgctggcg gtacgaactg tccgtgccgc
    26341 atggcgcagc cggctcagcc ggccggagca gattgctgcg ctggatgtac agacggctca
    26401 actgcattta ttgttcggga tgcaattgat tttttccgtt ttgctggggg cttttttgtg
    26461 aaagcgctgc ttgtactgct gctggctgct caagcaggtt tttttgttct ttttagtccg
    26521 tggacaaagg acattccttt ttggcctgta atgactgtaa tgactgctgt gcttggggga
    26581 ggggcactgg ctttggattg gcagatgctc aaggcggttt tccgatttga ctgttctgct
    26641 gttattctcg gcgttggaag cgcaattatt ttgtatgggc tattttggtt gggatattgg
    26701 atctcatcgg ctgtcctgcc ctttgccgca tcgcaggtgc aatcggttta tgctgtgcgg
    26761 gctgggcaaa atccatggat tatcacagga cttttatgct tgattatcgc tccggcagag
    26821 gagatcttct ggcggggctt tgttttgcat cgactatcaa ggcaattcgg ttttgcagcg
    26881 ggacttgcgg ctgctgcagc ggtatatgcg ctggttcata tctggtcgtt taacctgatg
    26941 ctgtcggctg ctgccggcat ctgcggtgtc tattgggcgt tgctgttttt cttgacccaa
    27001 aacctttggt cttgtattat cagccactgc ttgtgggatg taatgatttt tgtgctgttt
    27061 ccaatccaat gacaaatgaa aacgcacaat ttgtttttga tgcaatagcg gccgattacg
    27121 accggtttaa tcggctgttt agtttcggca ttgaccgctg gtggcggcga aagcttgtag
    27181 agatggtgcg gccgcatcct catcagcaga tactggattt atgctgcgga acgggcgaag
    27241 tgattttcag ttttttgcgg cattcgctgg tgcgaacggt tgcaggggca gacatcagtg
    27301 aagcaatgat tgattttgca atcgaaaaac agatacagta cagcacaaag ccgtggatgc
    27361 agaacaagtc gcttgcctgg catgtctgcg atgcagccca aaccggtctg gcggccggca
    27421 gttttgacat tatcacctgt gccttcggcc ttcgcaacat ctcggactgt tcggctgttc
    27481 ttaatgaaat gcgccggctg ctcaaaccca agggcaagct gggcatactg gaatttgcgc
    27541 tgccgagcca tccgctggcg ggaaaggttt atgactttta tttgaatcgc ctgatgccgc
    27601 atttggccaa gcccgtgctt ggtgcgtcag atccggtgcg gtatctggct gactccatcc
    27661 gccgatggca taatacggtt gattttgccg gccaattgga caaggcagga tttaaattaa
    27721 tctgcaaaac tcccttgacg gcaggcattg cagccttgtg gcttgcccac agccagtaaa
    27781 tggactcgtg ggctgttgtt aaaaagcaaa aacgccgata tagtatgcag gttttcaaaa
    27841 tgacagactg ttttcagcag gatatatgaa tgacatttac acaattattg tctttaaccc
    27901 agtcaagccg cctgcgggta tgtgcggatt cgcgtcaagt tcgcccggga gatgtatttg
    27961 ttgctgttcg cggtactcaa gccgatggac ataactttat ccggcaggca gccgctcaag
    28021 gagcggccta tattgttgct cagcagccgg tagcgggtat cgaatgtatt cttgtcgagg
    28081 acagtgctcg agcgctggga ctgctggcgc aggcctcata cgacaatccc aacagcaaac
    28141 tgaccaatct ggcagttacc ggaaccaacg gcaaaacaac cacgtcctac ttggttcgtt
    28201 ccgttatcga aagaaccgga gccaaatgcg gactaatcgg caccattgaa tacagcaccg
    28261 gttcgagcac aacggcagcg ccgctaacca cgccggatgc cgttaccttg gcctgtgctg
    28321 ccgacgaaat ggtccgcagc ggcgccgaat atatgatgat agaagccagc agtcatgcct
    28381 tgagccagaa gcggctggcc ggcatttcgt ttacggcggc ggcattcacg aacctcaccg
    28441 gcgaccatct ggactatcat caaacactgg atgattatct cgaagccaag ctgtcgctgt
    28501 ttatggattt gcccccgcat gggctggcga ttctgaacta tgaatcagac gaagcccaca
    28561 caattgcgga agtgatcgag ccggctacgc gcattctctg gtacggcatc gaccgtgatg
    28621 tggatctcat tgcccatatt catcggatgg atgcagcggg tagtacattc agcatcattt
    28681 tcaacagcat cagcgaaaaa gtaattacgc cgctgcccgg gcggcataat atcctcaatt
    28741 gtctggccgc agcagggctg tgtttggcag ctggttttac gctttcggat attgccgaag
    28801 ggctgtcgat tcttgaatcg gtacccggtc gtctggagcc ggtggacagc gaaggcgtac
    28861 agcgccgtaa aattggggtg tttgtggatt acgcccatac ggatgatgcg ctgaaaaatg
    28921 ttttaggaac gcttaaacca ttgtgtaaag gccggcttat tgttttgttc ggctgcggcg
    28981 gagaccgcga caagaccaaa cggcctcgta tggcgcgtgt agccgaacag atggccgata
    29041 tgattattgt taccagcgac aaccctcgta cggaagatcc ggtggcgatt attaacgata
    29101 ttttatctgg attttcagat ccgcacagca gccggctgat agtcgaaccg gatagagcca
    29161 aagctattcg ccgtgcgatt gaggaggccc gccccaatga tatggtgttg ctggccggca
    29221 aggggcatga aaagtatcaa atagtcggta cacagaaatt ccatttcagt gatgctgaag
    29281 aggtgctgaa ggttgttgga taatgtccct ttcaaaggta taggcaaaga tggatccgat
    29341 ttctctccgg cagcttattc aaggaattga aggccttcag ctcaatccga gtgtacagcc
    29401 gacagcagat atctgcggca tcagcattga ttcccgaacg attgagccgc agcaggcctt
    29461 ttttgctatt tctggtcaga actttgacgg ccattcatat gcggcagcgg ctattgaaaa
    29521 aggggcagcc tgtgtgattg tcgaaaaaat agcaatgccg ccgcaggagt gccgtgttcc
    29581 gatgatttta acaaatagta cggttgcggc actgggacgg ctggctgcat ggtaccggca
    29641 acagctttct gccaaagtca ttggcatcac tggttctgtt gggaaaacca cgacacgcca
    29701 tatcctttat caggtattat ctgctcgtta caaatgccga caagcccaaa aaagttttaa
    29761 taatcatatt ggtctgccgc tgacaattct ttctgcccaa aaagatgacg acattctctt
    29821 gctggaaatg gggaccaatc atcccggtga gatcgctgtt cttacgaaca tagctaatcc
    29881 cgatgccgcc gtgattacgc gggtcagtcc tgcccatctg gagggattcg gctcggtgga
    29941 ggcgattttg aaggaaaagg cctctattgc ccttggtgtt aagcccgatg gcacggtgta
    30001 tctgaatggt gaccagccgg aattagcgac gcatataaag actatttttc ccggccgagt
    30061 cgttacattt ggaacaacat cagactgtga tattgctgga acagatttgg aaaccgaagg
    30121 atgcagtgga tatctttcca tagaaggccg gcgtatcaaa gtaccgctgg ccggcagggc
    30181 aaacctgatg aacgttttga cagtttgggc aatttgcaag gattttaata ttactttatc
    30241 ggacttccaa catgctgtcg aagcccttac accgattcca atgcggcttc agatttattc
    30301 tctaggcaga ttgacgatat tggatgactg ttataacgcc aatcctgttt caatggccaa
    30361 tgcgctgtcc tgtctgcatt ctgtagcaag tcagggcagg cgaaaggttt ttttagccgg
    30421 ttgcatggcg gaattgggtg cacagtcggc ccagcttcat cgggaacttg gcgccgctgc
    30481 cactgccgaa ggtgttgaaa tcctgctggc ggccggtccc tttgcgaagg cggttttgga
    30541 aggagcagtg caggccgggc tgccgcctga acggatggca gcatttgaaa agacagacca
    30601 gttgtgtgat aatctgcata aatggattca agctgacgat ataatattaa taaaagggtc
    30661 acgttcggcc ggaatggaaa agtctattgt ccggctgcgg gacttgtttg aaaagcagtg
    30721 tatatcgaac gcaagggagt gatccttgat ttatcatctg ctatggtggc tacgggatat
    30781 cattacgcat agactcggct tctatgcata tcaggacgtg ctgtttcgag cgatcctggc
    30841 tgccttaacc agtctggcca tagccgtgct gctggcgccg cggatgattc gctggctgat
    30901 gcggatgaaa gtcggggacc ggccggagtt tcatcatgca gccctcaacg aactgatgcg
    30961 cgaccgtgcc aatacgccga caatgggagg gattattatt ctgacggcaa ttctggcagc
    31021 gacggggctg tgggcacgtc tggacaatcc gtttatccac aaagcagttt ttatcctgat
    31081 ttggtatggc ggcattgggg ctatggatga ctggctgaag ctgaccagca aaaaccgcca
    31141 gcacagccgt gatgggctca aaccgtggga aaagctggtg tttcaattcg ggggagctgt
    31201 tttagtggct atctttctgt attcggattt tcgccagata gaagatgccc gccggctgtg
    31261 gctgccgttt tataaacatg gggtaccgct ggccggatgg gtatttgcta tcgtgacggt
    31321 tctttatatc tcggcgacca gcaatgcggt gaatctgacc gacggaatgg acgggctggc
    31381 atcgggatgt ttgagtatta tgtccacagt gttagtgatt ctgtgctata ttgcttcgga
    31441 gtatatgtct cgctcggcac aggtcacttg ggccagctat ctgctgctgc cccatgtgcc
    31501 gcagtcagga gaactgtgca tcttttatgc ggcaatttcg ggggcaatgc taggcttttt
    31561 gtggtacaac tgccacccgg cgcaggtatt tatgggcgat atcggttcgc tgccgttggg
    31621 ggcagcgatg gggtacggag cggtggtaac ccgcaatgaa atcttgctgt ttgtcatcgg
    31681 cggtgtcttt atgatggaac tgttcagcgt ggtgctgcag gtggcatact tcaaatacac
    31741 cggcggcaaa cgcatcttcc gctgtgcgcc gattcatcat cattttcatt tggccggctg
    31801 gtcggaaccg caggtagttg tgcggttctg gcttttgtca gttgcctttg cggtcttggc
    31861 gctggcaacc ttgaagctgc gataagtacc ctgcgggaga tgcaatgaaa ctatcactgc
    31921 attacggtca ggggagggtc gaactgatta ttccgcaagc caatttggcc ggatttattc
    31981 agccacggca ccaggcaacg ccatctgacg gcagacagct gctcctgcag gcaatagagc
    32041 cctgccgtag gcagctggcc agccggacac aggggcgatg tgtgggcatc ctgcttccgg
    32101 acggtacgcg cgatttgccg catcaggaaa ttctgccgct gcttctggaa ccgcttggca
    32161 gggcacggaa actgcttttt tttatctgta ccggcacgca ccccggcaat accccggcca
    32221 atcaggaaat cctccgtacg atagcagcct gctcgatgaa tttgcctgcc cgctgggagg
    32281 tgattgtaca cgattgtcag gacgcctctt ttcagtcggc tggtcttact aaacgaggga
    32341 cagatgtgct gtataacagc cggctggacg aagcggagat catcctttgt ctgtcagata
    32401 ttaaacacca ttattttgcg ggctattcta atccgataaa aaacatagtg ccgggtttat
    32461 gttctttttc tgttgctgag cagaatcacc gctggacgct ggatgcccga agccgtgccg
    32521 gatttcatcc gtggcatccc tgtgccggcc gtcaggaaaa tccgctggcc tgcgatatgc
    32581 tggaggcagc agagcggatt gtcagggatc ggccgttctg ggcactggcg gtcatcagca
    32641 gccgcaaggt tattcagtgg gccgatttgg gcttggcgca gcaggtatgt gcgcgggctt
    32701 ttcagagggc cgatgaatgg aatgtgcata cggtcgagca gacacattat atggtggttt
    32761 cgccgggagg gttacccaat gatgtggatc tttatattgc ccagcgtgca ctggaattaa
    32821 cgcacagcgc agcagctgac ggcggccata ttttgttttt ggcggcctgt cccgacggca
    32881 tcggcagtcc ccagacccgc gaccagttcg aacggccatt gacagagccg ctggaatcca
    32941 ttttaaatcg gcggccatca gagtatcgtc ttttcagtca taagccgtat cggcaggcaa
    33001 tgctgattcg gcgtttgagc cgcctgtgga tgcatacaga aattgactcg gctactgtcc
    33061 gcgccatcca tatggaaccc tgtacggatc ctcagcaggt aatcgacgat tggctggtac
    33121 aagagcctac agcacagatt ttggctgtgg atggagccaa taagctgctg ctggagtgtc
    33181 gaacaattta gagaaaagcc ctgatacggc tacggctggg gtggagtgcc gggctgcagc
    33241 tgaggcggct ggacctccgg cagaaaattg ccgtcggctg ggtctggcat ccggcggcgc
    33301 ggtctgccgg gcatctcagg acgctgacgg tcaaacggcc ccatccgctg gtcatctctg
    33361 tcgcggcggt catcgccggg ccccatccgg ccctcgtctc tgtctcgccg ttcctcgccg
    33421 ggacctctcc gatgcattcg cctgaagcct tcctgcatcc gcttaatctg ctcctgccat
    33481 cgctgttttt gggtttcatc aagggtttgc aggatttctt cattcatcgc ctcgatttcc
    33541 tgccggattt tcggctgggc ttcggctcga agggcatcta tggcgttcat atgcttggca
    33601 ataataggct tgatctgctc ctgctgttcg acagaaagat gaagctcctc ggccattcgc
    33661 tcgaccatgc ggcttcggat gaattctgga gccggcggca gttccggcat tgccggacgg
    33721 ggtaacgcca gccacgtcag ccccgatccg ataacaatac cagacacgag aatcacgatg
    33781 ctcaacagca ctggcttgag catcggcttt tgtatccgcg ttcccggatg ttgttcaatg
    33841 ttcattgtgc aacccaagaa acgaaggtca ttacttcgct gaccgaatca gctgcagact
    33901 gtcccaattg cgatacggca atcaggatgc aagctgctgc cgctgccgag aaagctccca
    33961 tccagacata cgcccggtat ggatcggctg tctgtgccat caagcaggac aaagcggcga
    34021 taacctcatc agccgcatgc actccgggtg ggcattcctg ccgggccagc gaaaccagtt
    34081 tgctgaatgt taattctttc attagtccat ctcaaattga atatgacttt tttcaagcag
    34141 ggccttgagt ttctgtttag ccctgtgcgt ctgcaccttg acaagtgtct gactccagcc
    34201 cgtccggcgg gctgtttcct ctacatcgca ctgctccaaa taccgcagcg tcagcaccag
    34261 ccgatcccgc gggggaagct gggacagcag ccgatgcaca atatcggcgg cctgctcggg
    34321 tgtcagggta tccggctcgg cagcggcagt ctgctcccat tgtccgtcat ccagtgacag
    34381 gtgctggtgg cgattgcttt gtttccagaa gcgataaccc gtgcgcgtag ctatgcgtgt
    34441 cagccaatgc tcaaacggcg cctgcgattt gtaggtatgc agggacaaat acgcctgcac
    34501 aaaggtttcc tgtaccagtt cttcgtggaa ggtcttgtcc cgtgtaaatc tccacagcag
    34561 ccggctgata tggctctggt accgttctat cagtcgacgg taggcctctg aatcgccttt
    34621 ctgcgaccgg cagatatcat ccaattccga aaacaagata ttagatgtct tattagaggc
    34681 tgctgatgcg aactgtactc cgtctatctg cggttttatc gctgctgtgt tactgccggg
    34741 cagtgcgcct ataaccaaat gttcaaacag atgttcaatc atctgtttgt cagtatagaa
    34801 catttggtct gaacaagcaa tcatttctgc ctcaatacag ccatattatg cattcacttg
    34861 ccaatgaaca tggttctctg cctgtatcgt cacatttcat ctgcatctct ataaaatagt
    34921 gaaaagcgga gactgttggt tacaaatttt tacttaaaaa tgtgaaaaaa gagggacttg
    34981 tatgtcaatt tagaggcagg atatggacat ttcctgttgt ttattagagt ttttcaatgc
    35041 aataggcaac atggatagaa agcagaatca gataatccat ttcggccagc gaataatggg
    35101 gatggtcagt agaattggca acataaataa caccgtggca ggtgtgggca gtcaaaatgg
    35161 gggtgatcat cactgagcgt atgccgcgtg ctataatctg gcggggcaat tggtgaatca
    35221 gcaggtattt attgccggcg agggcctcag ccagactggc cggaactgcc aaatcagtcc
    35281 ggtcaatgtg ttcagttgta atctgtcggc cgccttcgat atccatgggg ccagcagggg
    35341 ctttgcggaa agccatccat acatgcttgg ctataaactg gcggaacagc aaatccatca
    35401 ggtttttata cagcatatcc agcttggtgg ctttggagat aagctcgatg gcctcatgat
    35461 actgcttgat ccgcttgacg gcaaacttga cagggggggc atcgagggca tcaaagttcc
    35521 gctcgaccgt gtgcagatcc cgcgctacag ccgtatgctc gacagtaagc gtatcctcca
    35581 gctcattgcg gcggttttgg tcttcatccg gttccagact gacgcgaatt aaaaaatcgg
    35641 ctatccgcac gacatcgttg tgttttaact cgcttttgtg aatcggatga ctattcagat
    35701 aggtcttatt ggatgatccg aggtcttcta tgacccagtt tccgtcttta gtggtataaa
    35761 aaacggcgtg ctggcgagaa acggctttgt cgggaaggaa aacctgactg cccatctgac
    35821 ggcccacata gacgggtcct tggtcgaatt tcagttcatt gacgctgctc tggccaagac
    35881 tgataaaaag ccgcataaat gaccctttcc aaaagcttgg acaaccctca gtttaccaaa
    35941 gtttcatcca gctgtcaatt tttcttgaat tttatgcctt cagtatgtaa tctttggatt
    36001 gccaaaggat tacgtcctaa taaaggaccg ctctggagga gtgaccgagt ggcttaaggt
    36061 gacggtcttg aaaaccgttg cggggcaacc cgccgggggt tcgaatccct cctcctccgc
    36121 ttcatttttg ggctgaaatc gcggtttttc gcaaaatgtc ggcgatttca gccctcttta
    36181 atgcgccttt caattcaggc gacttttgcc aatctttgcc aggaaccacc cctttgcgtc
    36241 catagacagg gggtacagta gggggtaaaa gttgggggca ctttgcaaaa gtcagcccgg
    36301 ctgtgcccgg tttccggatg agccggccgt aacggcaaac acaagccaga tgtcgcaaca
    36361 caggcaaacg tggccagcgt ggcagcctaa attgcgccaa gatggagcag ttgcaggcgc
    36421 tggacaacag gttcttagtt tatggacgag aagaactcat cggtattagc ctgcaagaca
    36481 ttaaacataa ggtcgttgat tttccgcatg gccgctctaa acaattatgc ggccgtatcc
    36541 tcgagcaggc tcaggtcatc gtagccgagt gattttgtcg catggaaata acttgagagc
    36601 gtgaaaagat tgtaaatatt ttgctcacaa caacttatgc tctttgcacg caataaccag
    36661 tatttcattg aaaccagata gcaatttttc attgaccgat aagaagcgat tttatatcat
    36721 tattagttga aaactgaata ttgaaagtcc actgggcttt gagcgtctcg tcgtaaatcg
    36781 ctacatttac cgtgaagagg cgtgcaaaga tggtgcccca gaaggcgagt gccggtagcg
    36841 gtgctccctt tttcatgggg tcggctccat aaaaagggct tcaatcagaa aatggggggt
    36901 tattgaaagg agattggctg tgatagggaa gaaaactttt atcagggctg ccgtttgttc
    36961 aatcttgtca cttgctactt tttgtcatgc caaatcagtc tatgtcattg gggacacgga
    37021 caacagcacg atactgacgt acaaagtcgg tgtcttacgc aaaaacccat ctgacgcctg
    37081 aattggacgt tttgtgagat agacgcaggt tggaattgct ctttcataag ttcagatgga
    37141 cagattttgg gcgggttccg ctgaacccaa cggtgaaaat tctttgattt ttcgatatcg
    37201 gcgcagactg tgggcgtggt gcctatgaat agctcacatt tcaggcaacg cgggtataga
    37261 cgtgatgcag gccgccgagg acgggtttgc tcacaatttt gccgtaagtc tgaaccgggc
    37321 gagggacagg cgagtccttg ttcagcgaca tgtgagtgcg gctgacgttg taatagttgt
    37381 caatgtaatc ggtcagcacc gaccgcaggt gtttctcgtt caggataatg acatgattca
    37441 ggcactcttg tcgtagtgtg ccattgaccc gttcggcaat tgggttctgc cacggggaat
    37501 gcggcgcggt gggcgtgtct tttaggccga agcgtttgat atgctgcttg aactcttctc
    37561 tgaagatagc gtcattatcg cggataacat atttggtgga ttcgtcgaat gcaaaggtct
    37621 cacgcagttg ctggatggcc cattgggagt gcggcttact gctaatgccc caatgcagga
    37681 ttcttcgccg ttcgtgcgaa atggcaatga atacatagat tgccctgaag aacagcgtcc
    37741 ggacgacaag gaagtcgatg gcagcgatac agtgggcgtg atttttcaaa aaagttcgcc
    37801 accgctggcg tttgtcggcg tcaggtttcg gcttccgtag gtatttaaga acggtgttgt
    37861 cgcagacgtt aaatccgagt ttggccagtt cgccctgaat ccgctgcggt gaccagagcg
    37921 gattttcctt ctggagcttt cggataagct taatcagcgg ccagtcaatc gctggtctgc
    37981 cggcttttcg tgacttccag cgccagtaga gcctaaatcc cttacgatgc cagccgatga
    38041 cggtttcagg tttgacgatc accaacgatt ccttccattt ggaaaagact ctggacagcc
    38101 agaccaaaac aatgcgatcc tgtggcttga gtttgggtcg tttgaccgac tgctggcaga
    38161 taatcaactg ctgccgtaac aaggcatttt cgagcaatag ctgttttcgc gattggaata
    38221 aactcagaaa aagcttcaca aggctaatta acatcgtttc ctctggattg taaactttcg
    38281 cactttttag gcgactaatt ttaatggttt atcaacaaaa aca
//
LOCUS       DAOTBO010000017        37973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_871_length_37973_cov_5.662693, whole genome shotgun sequence.
ACCESSION   DAOTBO010000017 DAOTBO010000000
VERSION     DAOTBO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 37973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37973
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_871_length_37973_cov_5.662693"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="PLE88_03775"
     CDS             complement(<1..1884)
                     /locus_tag="PLE88_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOM75618.1"
                     /translation="MTAWTEAQKAAISAVGRKVLVTASAGTGKTAVLAERAVRRISDS
                     LAPVHADAMLVLTFTDAAAEEMRSRIAEALYKTYRQTAAKNLRLELLRLDRAYISTIH
                     AFCKRILLEFFFLIDLDPAFGIVDEDEQRLVKHEVLTEMLEEAWADEQLAQAMRTLFD
                     GRRVQAGSRSFIDRIIPLSEFLDSVVSRDDFYRRAAEISDIHSQAYRQLQDTQKEILL
                     QELAFCRQKLSAATALDSQWCNGQYVSDYIQQTVLPAVLKCEQLIGQEKYEECRQYIA
                     GINKWGQLRKKDKINQSQQELIKDLIKAVKDQLMALCDFALLADGYSQWIAPQAGLQT
                     QTLLELLKRFDRCYRAVKQRRNVLDFADLEHRALALLESHPDVSAQLRKRFEHIFIDE
                     YQDINAVQQRLIEHLARPDNLFVVGDIKQSIYGFRQSRPEIFLSHLESAADIAQPSSQ
                     PGRVDLQDNFRCRPEIISCVNALFERVMKTSVAGMDYDHRAHLRAAFPYEPFRSSAGP
                     AHPVELYLLDREENGQADSDDTSDSDADFSQTLSEVQRQAAFIARRIRQMVGSQPEFS
                     IFDKRLNAYRPAAYRDIVILLRSPAGRAQQYVEILRLAGVPVSSQSSCGFFETTEITD
                     CLCL"
     gene            2149..2928
                     /locus_tag="PLE88_03780"
     CDS             2149..2928
                     /locus_tag="PLE88_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOM75619.1"
                     /translation="MSLGQILRKRREQLKLTLDEVAALTGFSKPYLSTIETGHVKNPP
                     SDKLLRKLEEYLKFEPGHLQHLAHVERMPVDIRHEYEAIDAENRRFRQIIKDIIAHKI
                     NPEKLDKLLNGCDLQADAESPYREKPGQWVPIINKVPAGYPAEFDDMGYPVGFGDDYV
                     RCPDIHDPNAFAVRVVGDSMQPKFAEGDVVIFSPAADIQNGDDCFVRLREPHETTFKR
                     IFFEPDNRIRLQPRNNLYAPVIVESGRVNGLYRAVIHYQKL"
     gene            3090..6158
                     /locus_tag="PLE88_03785"
     CDS             3090..6158
                     /locus_tag="PLE88_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75620.1"
                     /translation="MANSVLIMVSMALCSGLAAETQPQTAPDLAGQDLHLTAPTMTVC
                     HQPEAEWPQVILLEGGAAVQIGDNLLSSRSAVIFLRPQGREQAAFGIGQAWQTRVYME
                     GDVSVQQGSRSKATPIQHFIVEGAQAIVTQFLATGQVFATADTNTAVSFDYLADSPLY
                     QQAVKAGQQIPLGPAVRKTARVPSPEEVIAHPEIQPEKTASRMATSIAQQIEHSRKPD
                     KSAPTGPVYPVHLSAVWEPVPQIQKTQMPDGQEVVTASGRFYLWQKRPDDTVIEFMAD
                     QLVLFFGKGQFELESDASKSGQLGMGTIQAVYLSGNIVMTEGRRTTRADELYYDFANQ
                     RALVVNASMRIFDEKRGLPVYLRASLLGRVSKDIFEAQDVQLTSSEFYFPQLSVNASR
                     MVLLTGEALEMRRRLTEQKDTAAQYEGQLKDVTVRYGSTAFFGWPSMVTNFARPDIPL
                     SRLTVGSDSEFGTAVETRWHLARLLGIRDPAWANSRLAADYFSDRGVGAGIENEYETD
                     DSKGSLISYIMTDRGKDDLGRYRKNIEPDKDIRGRFSFRHRQYLPDDWQLTLETSYLS
                     DRNFLEAMYRNEFYTDKDQETVLHLKRIWDNQAFSILGKIRINDFETTTDELPSVEYH
                     RTGQSFWDDRLTWYSDSQLGRFRNRFDNDADLRPTDTSDFYTFAYTRNEVDWPLMWNT
                     VKLVPFAAGSYGYEDDYGFDRTLDGRRIDSENKVILGEAGIRASTMFWKEDPFVRSAL
                     WDISGLRHIITPYMEAVVYEASDKTADMRDVIHAGIQQRWQTHRGSQQELRTLDWMRL
                     DIETTWVSEDAKAAVSPLADDPFGYGLRQVYGPAGFIYNDPSIPLLLRRDDSFFGTVR
                     DTLNTEYIWRISDTFSLLSDMNYDINSGRIQQLDAGVSRYVYPDISYYLGTRYLYPVV
                     LPVDKTRDGIPDIYEKGSNSFVAAVTYRLSQRYTATFSQEYNFDFGKAIRSDLALIRH
                     YHRLFYAMSLSLDESLNRSSVMFSVWPQGVEELTVGSRKYTALTGSRWED"
     gene            6213..7163
                     /gene="fba"
                     /locus_tag="PLE88_03790"
     CDS             6213..7163
                     /gene="fba"
                     /locus_tag="PLE88_03790"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOM75621.1"
                     /translation="MALVTTKKMFKMAYKNGYAIGAFNVNNMEITQGIVAAVAEEKAP
                     LILQISRGARSYASMSYLKAIIDVAVRENPDIPICMHLDHGDTFETCKQCVDDGFTSV
                     MIDGSHHPFEENIRLTKKVVEYAHDNGVVVEAELGQLGGIEEDVVGLSHDDVLKHLTD
                     PDQAVEFVERTGVDSLAVAIGTSHGAYKFKSAPKLAFDVIEKISEKLPGFPLVMHGSS
                     SVLPEFKELINKYGGKMPDAMGVPEEAISKAAKMAVCKVNIDTDLRMALTAKIRQVFA
                     EKPGEFDPRNYLGPGREAIKQMVKHKLHVLGCAGKAAECL"
     gene            7269..9506
                     /locus_tag="PLE88_03795"
     CDS             7269..9506
                     /locus_tag="PLE88_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOM75622.1"
                     /translation="MKPGKKTFISIGIIVLGILILAGSSCNPEQETRNGASSDKKKSS
                     SAGPSNTVLDAAVESICRGEFVQAEQLLAPLSDTQQAQQLLHLIQCYYQIEQHRQEEK
                     AAAYQEQSQKLKTIEERLSGKETIDANDIDETMAAVYQAREYAEDQEKETLLTRPFIQ
                     NLLSRMKAMGAEYEQQGKWIDAYAHCYYWLTLLYEDDPALRDKAEELADLAEIELSMK
                     DSSCGETAAQRYEGIQAVMLHRALQLLDANYVNGVDYRAMISKALVRCRRIGIMLAKT
                     QEKTAWTAPAEAIDRWNTGLEVLDKQLAEAPSEPLQMSEMTDFLEDVLALNAVTLKLP
                     DEVIIAHVTEAAFAALDPFTNMVWPWSVQDFEKSMTQQFTGIGVEISKATGVLKVVSL
                     LPDTPAYKSGLDADDEIIAVNGEPTDEMTIFCAVSKITGPKGTKVTLTVRRPSTGQVK
                     DVTITRDKIVVQPLRGWTREADGRWNYFIDPENRIGYVRLTSFTETSGPDLDEVLSKL
                     EKNGLSGLILDLRFNSGGYLQSAAEVVDLFVREGIIVKSSPRYGLDTYEIAHRSGTHP
                     DYPLVILINGSSASASEIVAGALQDPEHRRATLVGQRTYGKGSVQVVTGFTGGGSQLK
                     YTVAYYHLPSNQQVKNRYQMEKLGRKDWGIAPDVTVEMLRNEVQKMLDIQRDNDVLSQ
                     TDHPENGAGSRRHQLEETLRADPQLSIGLLVLQSKLIAQGRTLALTQTAADNRPAEPN
                     AVNVP"
     gene            9520..11013
                     /gene="lysS"
                     /locus_tag="PLE88_03800"
     CDS             9520..11013
                     /gene="lysS"
                     /locus_tag="PLE88_03800"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOM75623.1"
                     /translation="MSEDAFTDISKFERQRREKLQKLRQWKIDPYGGRFENAVPAETV
                     KAGYTDGQEGQRARCAGRIVLLRDIGKLIFVTLRDSSGTIQLGFSKQLMAQQWDFLKL
                     LDLGDIIGAEGQLGKTKTGEITIWTDSVTLLSKALLPPPEKFHGLADPDLRYRMRYVD
                     LWANPEVMKRFKTRSAIIASIRQFLNERGFLEVETPMMQAQAGGAAARPFITHHNSLN
                     LDLYLRIAPELFLKRLLVGGMEKVFEINRNFRNEGLSTRHNPEFTMMELYEAYADYRV
                     MMDLSEELISRLIETHVGSWKLPFGSMEIDYSRPWRRARYADLLAEHAGCRIDDMPSV
                     RAKARQLGIPETNMDDAVVINAVFEETVESHLINPTFVMDYPAAICPLTKQSKADPRY
                     AERFELFIGTMELANAYTELNDPDVQERNFRMQLRGLSEEESMAKMDEDFIQALKYGM
                     PPAGGLGIGIDRLIMLLTNATSIRDVVLFPLLKPLAVKTADTAENTD"
     gene            11114..12469
                     /locus_tag="PLE88_03805"
     CDS             11114..12469
                     /locus_tag="PLE88_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOM75624.1"
                     /translation="MKQISVLLCFRYLQHRRIVLLSIAAVALSCALLIATDSLFTGFI
                     SAVENSVGQHLGDIILEAPANRTITDYEVLIASLQQAACIRSATAVLSSQGLLLSAPG
                     KVRAVRVWGIELPRRLEVTPLQESLLFQKDKSAQQVSFDVPGAAEMTGGIVGIGILTR
                     PDEMTDEYDIEAVKGMLGQPMMLTCGAASYEQDSQDRTNGRPRFSRRVVRFALADVMM
                     TGVNDFDESFVMLPLDYLSRQLNPQSPPAANIIHIRLADGADEQEAMAVVRGLWNTFA
                     QTRLEWYNLASIESARAMQARLIAEYKKQMGVLLLIFGLVSMGIILLVFCIFYLIVMT
                     RRKDIGILKSCGLSSGSVAALFVLFGTVVGLIGAAAGIGLGWLIIDNINAIEQAVASV
                     FGIKLWKASTYLFSRIPNTMHWGSVVWITACGIGAAAAGSLIPAVAAARIRPVEILQY
                     E"
     gene            12527..13879
                     /locus_tag="PLE88_03810"
     CDS             12527..13879
                     /locus_tag="PLE88_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOM75625.1"
                     /translation="MIKWLLAWRYFIGRPISILAVAAVTLCVFIVVVVMTVMNGLVED
                     FKQKNHSYAGDCVIESDSLTGFAYYEEFLETLGRQPFIHAASPVVKGVGLLTMPGADW
                     NIGIEIHGIDPVLHSQATGFADTLYYHKDAPADAFRPSYLPETAGCIVGIDIMSPRRQ
                     SDGGYYHPDTPVALELIISAFPLNLRGGLARAGTDFISTKSFYYSDDSHSGLVKVDGA
                     VVYVPLAEAQILCGMDAPLKRASSIHIRFAAGTSPLDALEKVRTLWSEHQSRYGSQPG
                     GELLTHVRVQSWQENRRSIIAPMEKEQTMMIMLFIMLGLITVFIVLVVLYMIISHKSK
                     DIGILKSIGTSAAEMLGVFLLYSVFIGLIGAGLGSLGGWFFLSKINPLEDWLFEHYQW
                     QLWDRAVYAIGDIPHQVDADVLAAVIGAAVLSCLLGAIIPSYQAARKKPAQSLQVNQL
                     "
     gene            13889..14593
                     /locus_tag="PLE88_03815"
     CDS             13889..14593
                     /locus_tag="PLE88_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM75626.1"
                     /translation="MEIILKATNLHKTYRMGKVRLEVLRGINLEIPRGQFAAVIGASG
                     SGKSTLLHILGALDKPDSGSVHYENQRLDRMRWSQLNHFRNRTVGFVFQFYHLLDELT
                     VLENVMTPAMVSAGIFRWIKTKASIRKRAYELLEQFGLKDRLDHKPYELSGGERQRVA
                     IARALINQPPLLLADEPTGNLDSRTGHGILDTLAELNRQGQTIIMVTHDERIAARASR
                     IIRLVDGKVKEVAVNQ"
     gene            14618..15475
                     /gene="ilvE"
                     /locus_tag="PLE88_03820"
     CDS             14618..15475
                     /gene="ilvE"
                     /locus_tag="PLE88_03820"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006185.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOM75627.1"
                     /translation="MGPKIWINNCLVDQAEAKVSVFDHALLYGDGVFEGIRVYGGRIF
                     ECKAHLDRLYESAKVIMLKIPMEMEGLTRAMEDTVAANGIREGYIRLIVTRGIGDLGL
                     NPFLCKKAQIIIIADQIQLYPEELYDKGIKVISVSSVRNHPLTCPPQVKSLNYLNNVL
                     AKIEAVQAGADEGIMYNHLGYVAEATGDNVFIVKNGIVYTPPIQAGSLGGITRRVVIR
                     LAKQENINVVETDLTRFDLYVADEMFLTGTAAEVIGVAEMDGRTIGSGNPGPITLRLR
                     KKFFEYAHS"
     gene            15456..16484
                     /locus_tag="PLE88_03825"
     CDS             15456..16484
                     /locus_tag="PLE88_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOM75628.1"
                     /translation="MNTPIPENAAFLGSWLTAGIDALFEPVRNELAEVQGLIAEQMQS
                     PEEEINSCLRFLRQRPGKMLRPALLLLCGKTIEERLTTEHRDLAAMIELVHMASLLHD
                     DVIDSADLRRGFPSANALWGNTAAVLLGDYLLCRAFSLGILCRLADAAKLLGQTAQTL
                     CTGELKQNLLKGRPSLNEQDYLQIIESKTAVLFACGCRLGAMASGASEAQEKALKDFG
                     LHFGMAFQIADDLQDILSTAAQTGKTQGIDRTQEKWTLPVIRWISQDGSDRQARINQW
                     SSATDVRVLINQMRSDGSIDYAFQQARQEVQQAKQQLDSLPDTPARQSLKMLADHITE
                     PIRTVLNA"
     gene            complement(16561..17337)
                     /locus_tag="PLE88_03830"
     CDS             complement(16561..17337)
                     /locus_tag="PLE88_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75629.1"
                     /translation="MKKLLVGIVLVGCAAAVASAADVKTTIDLTYASKYIWRGFDMLD
                     DASAWQPSVNFGFDNGFSANAWASYAGTGGNDQMSRVNATEYRYTLAYSGTYAESCPW
                     KTDYTVGWRYYDFIDMPTKDKDVQEVFAEFAMPSLLGDSGLVPRGAVYQMWDSKGEGL
                     NKDFSGTIYAMGFGYNFKLEQAPDLPMMFGWDIVYNDGTGGPDVDNDWSHMVWSLKTS
                     FTCPLTGGKVVPGVYFQNSFEDTVNNEDELWGMISYALTF"
     gene            complement(17559..19007)
                     /locus_tag="PLE88_03835"
     CDS             complement(17559..19007)
                     /locus_tag="PLE88_03835"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOM75630.1"
                     /translation="MTATKKRPGKTTLSETRKKKPSSASVATVKRPAKAFHTYEQAIQ
                     YLFDKTDYEKQQKLRYNVTAFDLERMNKLLKGLGNPHQKTKLVHIAGTKGKGSTATML
                     AKMVEANGYKVGLYTSPHVMTLHERIVVNSQMISRKEMLDLMNRVYPVIEKLSEANEC
                     PTFFEIMTALAFMYFADHQVDLAVIETGLGGRLDSTNVIMPELVGITSISIDHQNLLG
                     PTIDCIAKEKAGVIKKGVPVVTVPQDAAAMRVLKKYAAVQKAPLIVTGKDIDFSYRFE
                     SSREYGPHTRICLTTPSSRFEHLRVPLPGDHQAINCGLALAMLDQLKNRGYTIDDQKA
                     VMGLSEVRLAGRMEMISEDPRILVDAAHNAASIRALIQAIGQHVPYDSMVIIFGCNSD
                     KDIRGMLTELQYGADKVIFTRSNSPKAVFPTDLAEMYTELCGKMCQTALSLKEAIRVA
                     CSAVTREDLICITGSFYLVGQAKQMFHPEPSI"
     gene            19460..21466
                     /gene="tkt"
                     /locus_tag="PLE88_03840"
     CDS             19460..21466
                     /gene="tkt"
                     /locus_tag="PLE88_03840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOM75631.1"
                     /translation="MAKLTELDNLCIQTIRFLALDGVQAAKSGHPGMPMGMAAAAYAL
                     WQRHLKFDPAHPEWINRDRFVLSAGHGSMLLYALLHLFGYDLPLDELKRFRQWGSKTP
                     GHPEYGHTPGVEVTTGPLGQGISNAVGMAIAQKYLAARYNKETFNLFDYHIYVICGDG
                     CLQEGISGEASSLAGHLGLDNLIVIYDDNHITIDGETSLSFTENVALRYRAYGWNVLE
                     IGGDGHDIDALDGALEFAKAQSERPTLIKFRSHIGYGSPNFQDTHTAHGAPLGEDEIK
                     LIKKNAGWDPEQKFVVPAEVTAHTGKAKETGRKLYADYQKLWAAYARAYPELVKELQD
                     ALNGNVVIDEKKLPVFDPATPLATRAASGKTLDALMPSMPLVLGGSADLTPSNNTRFK
                     GAEDFQKTNRAGRYIRYGVREHAMGAIMNGIAVSKILRPYGGTFMVFADYMRPAVRLA
                     AMSGYPTIFVFTHDSIGVGEDGPTHQPVETLASLRIMPNLLTFRPADANETALMWKYI
                     LEHPDMPAATALTRQNLPVLDPAKYPGMKNFNKGAYVLIGQTKPDVLLLATGSEVALA
                     LAAHDKLAAEGIKAQVVSMPCWTLFDKQDDAYKESVLPSAVKARVGVEAGVENGWWKY
                     LGTCGQFVGMHTFGASGTYKACFENFGFTVDAVVAAAKKSIAKA"
     gene            21578..22675
                     /locus_tag="PLE88_03845"
     CDS             21578..22675
                     /locus_tag="PLE88_03845"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOM75632.1"
                     /translation="MEWNKIKRIAVMTGGGDCPGLNAVIRAVTKSAINRYGLEVWGVE
                     DGYRGLIEDRIGLLNYNDVSNILTLGGTILGSCNSSNPFSFPVRRGQRTIHKDVSDTC
                     IANLRKRGIEALVCIGGDGTMASAAAFAKKGLPIIGVPKTIDNDIWGTDITFGFNTAV
                     TTAAEAIDKVHTTASSHHRVMIVEVMGRYAGWIALHAGVAGGSDVILIPEIPYQLEKI
                     AQYVLARSKKGKRFSIITVAEGAKPKGGKAVVFKTIKDSPDPVRLGGISFVLAEQLGE
                     LTGLSCRAVNLGHIQRGGSPTPFDRVLATNFGHYALELLMKGQINQLVVWTEGRLGSV
                     PLARIAGKIKTVRPNDPLIAAAKAVGTSFGV"
     gene            22706..25969
                     /gene="mfd"
                     /locus_tag="PLE88_03850"
     CDS             22706..25969
                     /gene="mfd"
                     /locus_tag="PLE88_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOM75633.1"
                     /translation="MLDLGQDKTVRQIAARLADHNPALGPVTVSGTWGSFAPLLAAYL
                     LKTLKRPILYIRPHIDDADDAADDLQVFIGLPVQTFPVWESPDEITDAADEILAQRLR
                     LSLWMTRQQPPAEPFVVSTCVQALNQPVARPQLLFELGLSLSVGQTVEPDLVIGWLAD
                     HGFERTDSVDMPGQFAQRGGIIDIYAPVCTAGNTPNTAEAMPVRIEFFGDCIDSIRRI
                     DLDSQLSHQMLSSVQITAPPLSIAPEGTELFINLLPAETLIILEEPAEIAEVSDVFLS
                     RLEDVRGLYPWPAVYKAIQRFTTLHISRFAGSASPDFQLDVCSVQQFEQKAGSLWKDQ
                     QAVLAELLREARTQQVLFYCENQAEIQRVSEILQEKFTVIPDTFLLLSGFIHRGFMIR
                     SLNTLVVTHHEVFGQVSIRRRVQRIQGLSPVDTALDLKKGDYVVHLTYGIGKYCGIQM
                     MEKAGAKAEYITLEYADKIQIHVPISNMHLVQKFIGSSPKRPMLSRIGTKKWQRQKEN
                     VARGVAELAAELLDVQARRQQMGGFAFGSDTIWQKEFEEAFAYLETPDQTTAAQCIKD
                     DMASSRPMDRLLCGDVGYGKTELAMRAAFKAVQAGKQVAVLVPTTVLCVQHGRTFRQR
                     FADFPVAVEILNRFQTPKETRRILSGCRSGTVDILIGTHRLLSDDVAFKDLGLLIIDE
                     EQRFGVEHKERLKRFRVNVDILTMTATPIPRTLHMSLIGLRDISSLSTPPLDRRAVAT
                     KVCRFSPEVIRKGILFEISRGGQVFFLYNRVQTIEHFAHTIRAIVNDSSVRIDIAHGQ
                     MHKHQLEDAMIRFINGDTNVLICSTIIESGIDIPNANTIFIYDADRFGLAQLHQLRGR
                     VGRYKHRAYAYLLLPESRPITPIAARRLKAIEEYSQLGAGFRIALRDLEIRGAGSILG
                     PEQSGHINTVGYELYCRLLAEAVKRLKNEPIEKEIQTVIDLGFETYIPKSYIPSDRQR
                     LEVYRRIGTARTAEDIDRLRTELRDLFGPVPEQVNMLLDTAQIRLWADRWRIQSIIVQ
                     KPDIIFTFFDGASGIDLFARYSGTIRIPDPRTVHIRLEKSYFEIKTLLAFLRKILRQ"
     gene            26434..27687
                     /gene="glyA"
                     /locus_tag="PLE88_03855"
     CDS             26434..27687
                     /gene="glyA"
                     /locus_tag="PLE88_03855"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOM75634.1"
                     /translation="MSSALEQTDKVVFDLISQEEQRQAASIRMIPSENYVSKAVMQAT
                     GSCLTNKYSEGYPGKRYYEGQQITDQIETLAQNRAKALFGAQAANVQPHSGSPANLAA
                     YCALAQPGDTIMGLALPHGGHLTHGWKVSYTGKFFNSVQYELDPATGRLDYDKIADLA
                     KKHRPKILISGSSAYPRIIDFSAFAEIANRVGAYHVSDMAHIAGLVAGKVHPSPVPYA
                     DIVTTTTHKTLRGPRGGMILCRAEHEAAVNKAVFPGLQGGPHMHTISALAVALGEAMT
                     PDFAAYAAQIVRNAKRLAEKLLGYGFNLVSGGTDNHLILIDLRNKGLAGKPLAKALDR
                     AHIVCNYNSVPGDTAGPFNPSGLRLGTPAITTRGMKEEQMDQIAAWIDTISRNLDNDD
                     VISRIGQAVIELCRQFPLPDQFVRV"
     gene            27703..28767
                     /locus_tag="PLE88_03860"
     CDS             27703..28767
                     /locus_tag="PLE88_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM75635.1"
                     /translation="MAEHPLKLAAIGLSDEAVELLDIAQESGLYAIAAVGDERNERAE
                     KTARQFDCPAYTDYRQLLVQTSADIVFFGGPVYQCAEFIRPAMEARCHILKSCPPALH
                     FEQLAHFYRTAGKEHVCFITAQNGRFGLPFEHIRNYLSQIGQDDPHPWHLISAVCHVP
                     MGPPEQEMRWLYDPTMAGGGVLLHHCYDLIDQLILCFGLPQRVYALTINQAPDKQQRM
                     SLTEDTAVVAMQFTDALIAQICASRTLGPARWHLRVHGKLQHLTATKEEIIICDNLGN
                     LLEQKAYPNDERPSKMRMLQHLAAGLKNPPEQPLYPEHGFDLNTMAVIEAAYLSARTG
                     MAEEPARILKLAETGTASIL"
     gene            29250..30623
                     /gene="rsxC"
                     /locus_tag="PLE88_03865"
     CDS             29250..30623
                     /gene="rsxC"
                     /locus_tag="PLE88_03865"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOM75636.1"
                     /translation="MAKIRGLFTFRGGVHPPENKEYSEQCPIEVVPTPKQTAVLLSQH
                     LGAVCKPLVAKRDKVTAGQMIGKTDAFVSAPVHSPVNGTVRDITLLPHPVIGRTMGII
                     IDVDQQITVPKQPAAGQFDKNFDPSAYSVEDICRAAQEGGLVGMGGAGFPTRVKIEPN
                     PSMPKETLIINGCECEPFITCDYRLMLEWTWQVLAGIRLAAKAAGVSKIRIGIEDNKP
                     QAIGVMTEAVQSLGGTEDIQVVPVKTKYPQGGERQLIQAILKKTVPTGSIPPAIGICV
                     LNVATCAALAEAVVLHQPLTHRVVTVTGRAISRPGNYYVPIGISAAELIDYCGGLTEQ
                     AAKVVLGGPMMGFAIADLSQPITKTSGALTLLTRQDVTEAKFSHQQTPCIRCGRCLAV
                     CPERLNPTKIAHAVKHFRMDIAVQNYLTGCIECGCCSYVCPAHIELSGYIKTGKLLMA
                     REKKRLG"
     gene            30661..31671
                     /locus_tag="PLE88_03870"
     CDS             30661..31671
                     /locus_tag="PLE88_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOM75637.1"
                     /translation="MLSKITVSPAPHISQKRTTRGVMLDVIIGLSPAIAAGIFFFRTQ
                     AALVLAVSVGSCMFFEWMCNAIRKKPNSLGDLSAVVTGLILAMSLPPAIPLWAVVIGS
                     GFAIIICKMLFGGLGSNIFNPAMAARAFLTASFGLLMTTWTVPAPLDPSMPLLKAENT
                     LAVTQATPLAWSKEAIKHKASAQTANQQIGAAFFGTVGGCLGETSSLALLIGGLYLLV
                     RRTVTWHIPVCVLLSAAVLASLFYCVNPDRYASPLLHLFGGGMLICAFFIATDPVTAP
                     LTAKGMCIFGLGVGALIMLIRIVGEYPEGVMYAVLIMNALSPLIDRATPCIPAGGKPN
                     VQ"
     gene            31661..32275
                     /locus_tag="PLE88_03875"
     CDS             31661..32275
                     /locus_tag="PLE88_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOM75638.1"
                     /translation="MSNKQTKNRIVYFLEESWLLMVSSVVFGCLLAVFNALWSPRIEA
                     NQQSKFAQLAGGLLTGAVSFKEAGTFNGITVMKALTADGSPAGWAFVCEGAGFADKIK
                     LVVAVDTAFEKMAGFGVLSSNETPGFGDKITIKPEDGGFYQPQFIGAPVETLTLTKTG
                     DASKIDSEIVAISGATISSEAVVSILNASLLPVKEAMRQQGLLP"
     gene            32360..32974
                     /gene="rsxE"
                     /locus_tag="PLE88_03880"
     CDS             32360..32974
                     /gene="rsxE"
                     /locus_tag="PLE88_03880"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOM75639.1"
                     /translation="MSSNASIGKTFLAGIWDSNPVLRLMLGMCPTLAVTAAVKPAVTM
                     GLSVVFVLFCSNAVVSLMRNLLKPHLRILMFTLTIAAFVTIADLALKAYLPDMSAALG
                     PYIPLIIVNCIIIGRAEACASKNGLLVSIVDAVGMGLGFTLALCLLASIREVLATGKW
                     FDIPVMPAAFVPWAAMGMPVGAFVTLGLILAGVNLITKKNKEKN"
     gene            33016..33612
                     /locus_tag="PLE88_03885"
     CDS             33016..33612
                     /locus_tag="PLE88_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOM75640.1"
                     /translation="MDTAQTLLLGFIGIVIVNNLVFTKFLGICPYLGVSGRIDMAFGM
                     GLAVTFVVTLAGTLTWIIDHSFLIGTPLEVMRYLCFIMVIAGAVQLVEMYLKKFFPPL
                     YESFGIFLPLITTNCAILGMCLFINLWAIDSLLMAAVLSAGAGIGFTLAICIMAGIRE
                     NLQLADVPPCLRGAPITLITAGLLTLAFMGFAGMIKGS"
     gene            33698..34555
                     /locus_tag="PLE88_03890"
     CDS             33698..34555
                     /locus_tag="PLE88_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOM75641.1"
                     /translation="MLNILLAGIINNAWPAAALALGLSTVFAAVLLIASIKLKVQEDP
                     KVEQIHKALPNIDCGACGFAGCAAYAKAVAANPELLGKCAPGGSDTANAVAAILNLQL
                     SGSGAPRRPIIHCRACQQDKTYFAKYEGIIGCTSANAQPNVQACAFGCLGFGGCVRRC
                     KFDALHIIDGLAVVDYEKCTGCGACAAGCPRGLIEMVPFTHPIMMTVACSSRENGKNT
                     RAFCKVGCIGCGLCAKQSDLFAVSDNLARIDYARYAPSEAAQAAMDKCPTGAIVYRGT
                     NAPVPRQAG"
     gene            complement(34606..35637)
                     /locus_tag="PLE88_03895"
     CDS             complement(34606..35637)
                     /locus_tag="PLE88_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOM75642.1"
                     /translation="MLQERTRKSVQAVIGLFAAAALLWAAFKDRPVAIGSGWRMTMGT
                     IARIVVVTERPSDGLKAIEAAFEKIYDIEKRMSDYDPNSLLSKVNREAFESPVEVDAE
                     LFEVLAAAVEYSRLSEGAFDITVGPVVQIWRQARVTGAAPAPQQLEAAKERVGYGNLI
                     LDAQNRTVRFTKEGMFLDLGGIAKGYAVDKAVEILQKAGLRGGMVDIGGNIRCFGTPA
                     DGAPHWLIGLQDPASDKNILAKLKLDNRAVATSGDYRRFTIIAGQKHSHIIDPTTADS
                     AQTLSSVTIIAPTAMQADALSTAVSVLGDKKGMPFIESLPDVEAILIPRGEKPVFEKT
                     AGAGRYLLD"
     gene            35703..37496
                     /locus_tag="PLE88_03900"
     CDS             35703..37496
                     /locus_tag="PLE88_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOM75643.1"
                     /translation="MIHKQIQSIKDIISRQFLPYVRKPARYIGGEFNQICKDLNRCDV
                     RIALCFPDIYEIGMSYLGLEVLYSIINNLEGAAAERVFAPWFEAEEVMRSKKIPLFTL
                     ESHAPVRDFDIIGFSMTNELCYTNLLNMLDLAGLEIRAENRSDNDPLVIVGGQAANCA
                     EPISAFADLFVLGEGEEAAVELIKLYRRIQAANGTKQDYLEACAQQFSYVYVPKFFQV
                     NDAPDSSSYVKPTKEGVRDKFMNAVVRDFENAPIPEKPIVPFVQTVHDRVSIEIMRGC
                     PGHCRFCQASWCRRPVRVRSVGRIVEAAKKQYEATGYDTVSLLSLSTSDYPQLEELVE
                     AMNAVFSPLHVGLSVPSLRVQKQLQLLPKMVASVRKSGLTIAVEAASEKLRKVIGKPI
                     ADEDLLAAVRAAFEAGFLKVKLYFMVGFPGETEEDILKIADLSKTISALRKETDGKWA
                     DVNAAVSWLVPKAHTPFGWLGQRDRAYFEQARRLILDRKRQLNLRYVKFNFHSIERSI
                     LESAVGRGGRRLCEVIETAWRNGAKFDLWDEGFKFAVWQAAFASAGMDLEQEAAHALP
                     PGGTTPWGHLGGPDERYLLKEYQQAMTLVQS"
     gene            37498..>37973
                     /locus_tag="PLE88_03905"
     CDS             37498..>37973
                     /locus_tag="PLE88_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75644.1"
                     /translation="MMCKAHSKKNRVCFVSLKAYPLFNPDISSVFGGAEVDLYMLATE
                     LANDERFEVSFIVGDYGQPPLEVRQGVTLIKSLDAKKTKILHGWKIWKALRRADADVY
                     MQEACSLGTFLIALFCKIHRRRFVYRTASTQEADGTYRHDHPIAGRLFLWSLKNAAA"
BASE COUNT         9142 a         9561 c        10290 g         8980 t
ORIGIN      
        1 caggcatagg cagtcggtaa tctcggtggt ttcaaaaaac ccgcaggagc tttgagaact
       61 gacaggcacc cctgccagcc gaaggatttc gacgtattgc tgcgcccgtc cggccggcga
      121 acggagcaaa atgacaatat cgcgatacgc cgcaggtctg tatgcgttca ggcgcttgtc
      181 aaaaatactg aactcgggtt ggcttccgac catctgccga atccgccgcg caataaatgc
      241 cgcctgccgc tgaacctctg acagcgtctg cgaaaagtcg gcatcactgt cggaagtgtc
      301 gtcggaatct gcctgaccgt tttcttcccg gtccagcaga tacagctcca ccggatgggc
      361 aggtcctgcc gaactacgaa acggctcata gggaaaggcc gctctcaagt gtgcccgatg
      421 gtcataatcc attccggcaa ccgaggtctt catcacccgc tcaaagagag cattgacgca
      481 gctgatgatt tcggggcggc atcggaaatt atcctgcaga tcgactcggc cgggctggct
      541 cgacggctgg gcaatatcgg cggcggactc caaatgcgac aggaaaatct ccggtctgct
      601 ctgacggaaa ccgtaaatgc tttgcttgat atcgcctacc acaaaaagat tgtccgggcg
      661 ggccagatgc tcaataagcc gctgctgaac agcattgata tcctgatatt catcgataaa
      721 gatatgctca aaacgcttcc gcagctgggc ggacacatca gggtgcgatt ccagcagggc
      781 caaggcccga tgttccagat cggcaaaatc cagcacattc cggcgctgtt ttaccgcccg
      841 atagcatctg tcgaaccgct tgagcagttc cagcagtgtc tgagtctgca atccagcctg
      901 cggagcaatc cattggctgt agccgtcggc cagcaaggca aaatcacaga gggccattaa
      961 ttgatctttt acagccttaa taagatcctt gatgagctcc tgctgagact gatttatttt
     1021 atctttcttt cgaagctggc cccacttatt aatacctgct atatactgcc ggcattcctc
     1081 atacttctcc tgcccaatga gttgttcgca cttcaaaacc gccggcagga ccgtctgctg
     1141 gatatagtcc gatacgtatt gaccattgca ccactggctg tccagagccg tcgcagcaga
     1201 cagcttctga cggcagaaag ccagctcctg cagcagaatc tctttttggg tatcctgcag
     1261 ctgccgatag gcctgactat gaatgtcgct gatttcagct gcgcggcggt aaaagtcgtc
     1321 ccggctgaca acactgtcga gaaactcgct gagagggata atgcggtcga taaaacttct
     1381 ggaaccggcc tgaaccctgc ggccgtcaaa cagcgtgcgc atcgcctgtg caagctgttc
     1441 atccgcccaa gcctcttcga gcatttccgt cagtacttcg tgcttcacaa gccgctgctc
     1501 atcttcgtcg acgatgccga aagccggatc caaatcaatg agaaagaaaa actccagcag
     1561 aatgcgtttg cagaaagcat gaatggtgct gatatacgca cgatccagac ggagaagctc
     1621 taaacggagg tttttggcgg ctgtctggcg ataggtttta tagagtgcct ctgcaatccg
     1681 gctgcgcatc tcttcggctg ccgcatcggt aaatgtcaga acaagcatcg catcggcatg
     1741 caccggcgca agggagtcgc tgattcgtct tacggcccgc tctgccagta cagctgtttt
     1801 ccctgttcca gccgatgcag tcaccagcac cttccgcccg acagcgctga ttgccgcctt
     1861 ttgtgcttcc gtccatgctg tcatgtattt tatccctgcc cttctgactc tccgaaagtc
     1921 agccctactc taaacaaatc agaatgccgc atcaataccc ttatgacaaa aactgctccc
     1981 ccttatgtcc tgcaaaacag gcataatctt atgcaattaa gatgcgtctt gcatccaatt
     2041 ttttcggccg gatttaaaaa tccttgtgtt tcaggagttt tatttttttg ttggataaat
     2101 aacaaaatgg cttgcatatt gtactccacc gcattataat aggccattat gtcgttaggt
     2161 caaatacttc gaaagcgtcg ggaacagctt aagctgacac tggatgaggt ggctgcattg
     2221 accggctttt ccaagccgta tctttcgaca attgagaccg gtcatgtcaa aaatcctccc
     2281 agtgacaaac tgcttcgcaa actcgaagaa tatctcaaat tcgagccggg tcatttgcag
     2341 cacctcgccc acgttgaacg gatgccggtt gatatacgtc atgagtacga agcaatagat
     2401 gccgaaaatc ggcgatttcg gcagattatt aaagatatta tcgctcataa aatcaatccc
     2461 gaaaaactcg acaaactgct gaacggctgt gacctccaag ccgatgccga aagcccttac
     2521 agggaaaaac cgggccagtg ggtacctatt atcaacaagg ttcctgcggg ctatccggca
     2581 gaatttgacg acatgggata tccggttggt tttggggacg attatgtccg ctgtccggat
     2641 attcatgacc ccaatgcctt tgccgttcgc gttgtagggg attcaatgca gccgaagttc
     2701 gcagagggtg atgttgttat attttcgccg gccgccgata ttcaaaatgg cgatgattgt
     2761 tttgtccgcc ttcgagaacc ccatgagacg acattcaagc gtattttctt cgaaccagac
     2821 aacagaattc gcctgcagcc gcgcaataac ctttatgccc ctgttattgt cgaatccgga
     2881 cgtgtcaacg gcctctaccg cgcggtgatt cattaccaaa aactgtaagg atacaaaacc
     2941 catcctgtgt gactttcacc atgattattc cggtttttaa ctggaattga cagcgtgaaa
     3001 cgctataatc tctgacattt aatcctattc ctaaaaagga ctattcagga gcaggtaaaa
     3061 ccggatttat gtcattggga acaaacctca tggcaaacag tgtcctgata atggtgtcga
     3121 tggcactatg cagcggactg gctgcggaga cgcagccgca aactgcgccc gatttagcgg
     3181 ggcaggatct tcatctgacc gccccgacaa tgacagtctg tcatcagccg gaggccgaat
     3241 ggcctcaggt tatcctgctg gaaggcggcg cggcagtcca aataggagac aatcttttat
     3301 ccagccgcag tgccgttatc tttcttcgcc cgcagggccg cgaacaagcg gcttttggca
     3361 tagggcaggc atggcagacg cgggtgtata tggagggaga tgtttccgtt cagcagggca
     3421 gcaggtccaa agccactcct attcagcatt tcatcgtcga gggggcacaa gcgattgtta
     3481 cccaatttct ggcaaccggt caggtgtttg ccacagcaga tacaaacacg gcggtatctt
     3541 tcgattatct ggcagacagc ccgctatacc agcaggcagt aaaagccggc cagcagatac
     3601 cgttggggcc tgcggttcgc aagacagccc gagtacccag tcctgaagag gttattgctc
     3661 atcctgaaat tcagccggaa aaaacggctt cgcggatggc aacttcgatt gcccagcaaa
     3721 ttgaacacag tcggaaacca gacaaatccg caccgacagg accggtttac cctgttcacc
     3781 tgtcggccgt ttgggagccg gtcccgcaga ttcagaaaac ccagatgccc gatgggcagg
     3841 aagttgttac ggcttcgggg cggttttatc tatggcagaa acggcctgat gatactgtca
     3901 tagagtttat ggctgatcag ctggttctgt tcttcggcaa aggacaattc gaattggaat
     3961 ccgatgcttc caaaagcggc cagctgggaa tggggaccat tcaggcggtc tatctttcag
     4021 gcaatatcgt aatgacagag ggccgccgaa cgacacgggc tgatgagctg tattacgatt
     4081 ttgccaatca gcgggcgctg gtggtgaatg catcaatgcg gatttttgat gaaaaaagag
     4141 gcctgcctgt ttatttgcgg gcttcgctgc tcggacgcgt cagcaaagat atttttgaag
     4201 cccaagatgt gcagctgacc agcagcgaat tttatttccc gcagctgtcg gtcaatgcct
     4261 ctcggatggt gctgctgacc ggcgaggcgc tggaaatgcg gcggcgtctg acggaacaga
     4321 aagatacagc cgctcaatac gaaggtcaac tcaaggatgt aaccgtccgc tatgggagta
     4381 cggccttttt cggctggccc agcatggtaa ccaatttcgc ccggccggat attccgctca
     4441 gccggctgac tgtcggcagc gattctgaat tcggcacggc ggtggaaacc cgctggcatt
     4501 tggcacggct gctggggatc cgcgatcccg cttgggcaaa cagccggctg gcggcggatt
     4561 atttcagcga ccgcggcgtg ggagcaggaa ttgaaaacga gtacgagacg gatgattcca
     4621 aaggatcgct gatcagttat atcatgaccg accgcggcaa ggacgatttg ggacggtacc
     4681 gcaagaatat tgagccggat aaggatattc gagggcggtt cagctttcgg caccgccagt
     4741 atctgccgga tgactggcag ctgacgcttg agacaagcta tctgtcggac cgcaattttt
     4801 tggaggcgat gtaccgcaac gagttctata ccgacaaaga tcaggaaaca gtccttcatc
     4861 tgaaacgcat ctgggacaat caggcctttt cgattctggg caaaatccgc atcaacgatt
     4921 ttgaaacaac aaccgatgaa ctgcccagcg tcgaatacca tcgaacaggc cagtcgttct
     4981 gggatgaccg gctgacgtgg tacagcgaca gccagctggg gcggtttcga aaccgatttg
     5041 acaacgatgc ggatttaaga ccgaccgata ccagcgactt ttacaccttt gcctataccc
     5101 gcaacgaggt ggattggccg ctgatgtgga acaccgtcaa actggtgccc tttgcagctg
     5161 gcagctacgg ctatgaggat gattacggtt ttgacagaac gcttgacggg cggcggattg
     5221 acagcgaaaa caaggtgatt ctgggcgaag cgggcatacg ggcctctacc atgttctgga
     5281 aggaagatcc gttcgttcgc tccgccttgt gggatatcag cggactacgt catattatta
     5341 cgccgtacat ggaggctgtt gtttacgaag ccagtgacaa gacggccgat atgcgggatg
     5401 tgatccacgc cggcattcag cagcgctggc agacccaccg gggcagccag caggaactcc
     5461 gcacgctgga ttggatgcgt ttggacattg agacaacatg ggtcagtgag gatgcgaaag
     5521 cggctgtcag tccgctggcc gacgacccct tcgggtacgg tctgcgtcag gtgtacggac
     5581 cggccggatt tatttataat gatccgtcta tccccctgct gcttcgccgc gacgacagtt
     5641 tcttcggaac agtcagagac accctgaata cagaatacat ctggcgaatt tcggatacct
     5701 tctccctgct gagcgatatg aattacgaca tcaacagcgg ccgcattcag caactggatg
     5761 caggcgtcag ccgctatgtt tatccggata tcagctacta cctcgggacg cggtatctgt
     5821 atccggttgt tctgccggtc gataaaaccc gcgacggcat tcccgacatt tacgaaaaag
     5881 gatccaattc ctttgtggcg gcggttacct atcgtctgag ccagcggtac acagcgactt
     5941 tttcgcagga atacaacttt gatttcggca aagctattcg cagcgatttg gcactcatac
     6001 gccactatca ccggctcttt tatgcgatga gtttgtcgct ggatgaatcc ctcaaccgca
     6061 gttcggtgat gttcagcgtc tggcctcagg gtgttgaaga gctgacggtc ggctcccgca
     6121 aatataccgc cctgaccggc tcccgatggg aagattaaaa tgcttcgagc aggtaaatag
     6181 taactgttaa cctatttttt aaggaagttc tcatggcttt agtaacaacc aaaaaaatgt
     6241 tcaagatggc gtacaagaac ggctacgcga tcggtgcgtt caacgttaac aatatggaaa
     6301 ttacccaagg catcgttgca gcggtcgcgg aagagaaggc accgctgatt ctccagatat
     6361 cccgcggggc cagatcgtat gccagcatga gctatctgaa agcgattatc gatgtggccg
     6421 ttcgggaaaa tccggacatt ccgatctgca tgcatttgga ccacggcgat acctttgaga
     6481 cctgcaagca gtgtgtggat gacgggttta catcggttat gattgacggt tcgcatcatc
     6541 cctttgaaga aaacattcgg ctgaccaaaa aggtcgtcga atatgcccac gacaacggcg
     6601 ttgtagtgga agccgaactg gggcagctgg gcggcatcga agaagatgtt gtcggtctga
     6661 gccatgatga cgtgctcaag catctgacgg acccggatca ggcggttgaa tttgtcgagc
     6721 ggacgggagt agattcactg gctgtggcca tcggcaccag ccacggtgca tacaagttca
     6781 agtctgctcc gaagctggcc tttgatgtga ttgagaaaat cagtgaaaaa ctgcccggct
     6841 tcccgctggt tatgcacggt tccagcagcg tgctgccgga attcaaggaa ttaatcaata
     6901 aatacggcgg caagatgccc gatgcaatgg gtgtgccgga agaagccatt tccaaggccg
     6961 ccaaaatggc tgtctgcaag gtcaatattg atactgacct gcggatggcg ctgacagcaa
     7021 aaattcggca ggtctttgcc gaaaagcccg gcgaattcga tcctcgcaat tatctgggtc
     7081 ccggccggga agccatcaag caaatggtca aacacaaact gcatgtattg ggctgtgccg
     7141 gcaaggccgc tgaatgcctg taaccgattg caggccgaat ccaatcgact aaccgcggca
     7201 gaacggcttc ggccgttctg cctgctctat tgcttagaca agagcccaag acacgaggta
     7261 cacaatactt gaaaccaggc aaaaaaacat tcataagcat tggaatcatc gttctgggaa
     7321 tactgatttt agcaggcagc tcctgcaacc cggaacagga aaccagaaac ggtgcttctt
     7381 ctgataagaa gaagtcctcg tcagcaggtc cttccaacac ggttctggat gcagcggtcg
     7441 aaagcatctg tcgtggggag ttcgtccaag ctgaacagct tttggcgccg ctgtctgata
     7501 cccagcaggc acagcagctg ctgcatttga tccagtgcta ctatcagatt gagcagcacc
     7561 ggcaagaaga aaaggccgcc gcgtatcagg aacagtccca aaaactcaaa accatcgaag
     7621 agcgtctgtc gggcaaagaa accattgacg ccaatgatat agatgagaca atggcagctg
     7681 tctatcaggc gcgggaatat gcagaagacc aagaaaagga gacgctgctg acccgtccgt
     7741 ttattcagaa ccttctaagc cggatgaagg ccatgggggc ggagtatgag cagcagggca
     7801 aatggattga tgcctatgcc cactgctatt attggctgac gctgctgtat gaagatgacc
     7861 cggccctgcg agataaggcc gaagagctgg ctgatttggc agaaatcgaa ctgtcgatga
     7921 aagacagcag ctgcggcgaa acggccgccc agcggtacga aggcattcag gccgttatgc
     7981 tccatcgggc tttgcagctt ttggatgcca actatgtcaa tggcgtggat taccgggcaa
     8041 tgatttcaaa agctctggtg cgctgccgcc ggataggcat catgctggcc aagacacagg
     8101 aaaaaacggc ttggactgcc cccgctgaag ccattgaccg gtggaacaca ggattggaag
     8161 tactggacaa gcagctggcc gaagcccctt ctgagccgct gcagatgtcc gagatgaccg
     8221 atttccttga agatgtattg gctttgaatg cagttacgct gaagctgccg gatgaggtga
     8281 tcattgccca tgttacagag gcagcgtttg cggcactgga tccgtttacc aatatggtct
     8341 ggccgtggag tgtgcaggat tttgaaaaaa gcatgaccca gcaatttacc ggcatcggcg
     8401 ttgaaatctc caaggccacc ggcgtgctga aggtcgtcag tctgctgccg gatacgcctg
     8461 cctataaatc ggggctggat gctgacgatg aaatcattgc ggtcaacgga gaaccgacgg
     8521 atgagatgac cattttctgt gctgtcagca aaattacagg tcccaaagga accaaggtaa
     8581 cgctgacggt ccgccgccct tcaaccggac aggtaaagga tgtcaccatt acccgcgaca
     8641 agattgtggt ccagccgctg cggggctgga cacgggaggc tgacggccgg tggaattatt
     8701 tcattgatcc tgaaaaccgc atcggctatg tccgtttgac ttcctttacg gaaacatccg
     8761 gaccggattt agatgaagtg ctcagcaagt tggaaaagaa cggcctttcc gggctgattt
     8821 tggatttgcg gttcaacagc ggcggctatc tgcagtccgc cgcggaagtg gtggacctgt
     8881 ttgttcgtga agggatcatt gtcaaaagca gcccgcgcta tggtctggat acatatgaaa
     8941 tcgctcaccg cagcggcacc catccggatt atccgctggt tattcttatc aatggttcca
     9001 gcgccagcgc ttcggaaatt gtcgccggcg ccctgcagga tcccgaacat cgccgggcta
     9061 cgctggtcgg tcagcggacc tacggcaagg gcagcgtgca ggttgtaacc ggctttaccg
     9121 gaggcggatc gcagctgaaa tacaccgtcg cctactacca tctgcccagc aaccagcagg
     9181 tcaaaaaccg ctaccagatg gaaaaactcg gacggaaaga ctggggcatt gcgcccgatg
     9241 taacagtcga gatgcttcgc aatgaagtcc agaagatgct cgatattcag cgggacaacg
     9301 atgtcttgag ccaaaccgat catcctgaaa acggtgcagg cagccgccgg catcagctcg
     9361 aggaaacact gcgggccgat ccgcagcttt ctatcggtct gctggtgctt cagagcaagc
     9421 tgattgcgca ggggcgtaca ctggcattga cgcaaacagc tgccgacaat cgtcccgccg
     9481 aaccgaacgc cgtgaatgtg ccgtaaagga atttaagcga tgtcagaaga tgcatttaca
     9541 gatatcagta aatttgagcg gcagcgccgc gaaaaactgc agaagctgcg ccaatggaag
     9601 attgatcctt atggcggccg tttcgaaaat gctgttcctg ccgagacggt caaagccggc
     9661 tatacagatg gtcaggaagg ccagcgggcc cgctgtgccg gacgaattgt actgctcagg
     9721 gatatcggca agctgatttt tgtgacgctg cgggattcca gcggcacaat ccaattgggc
     9781 ttcagcaaac aattgatggc ccagcagtgg gactttctga aactgcttga ccttggcgat
     9841 attatcgggg ccgaggggca gctgggcaag acaaaaaccg gcgagattac catctggacc
     9901 gattcggtaa ccctgctgag caaagccctg ctgccgccgc cggaaaaatt ccacggtctg
     9961 gcggatccgg atctgcggta tcggatgcgc tatgtggact tgtgggccaa tcccgaagtg
    10021 atgaagcgct ttaagacccg cagtgctatt attgcatcaa tacgccagtt ccttaatgag
    10081 cgcggctttt tggaagttga aactcccatg atgcaggcgc aggccggcgg cgctgcggcg
    10141 cgcccgttta tcacgcatca caattctctc aatctggatt tgtacctgcg gatagcgccg
    10201 gagctgtttc tcaaacggct tctggtcgga ggaatggaaa aagtctttga aatcaaccgg
    10261 aatttccgca acgaaggtct cagcacgcgg cacaatccgg aatttacgat gatggagctg
    10321 tatgaggcgt acgctgatta ccgtgtgatg atggatctgt ccgaagagct gattagccga
    10381 ctgattgaaa cacacgtcgg aagctggaaa ctgccattcg gttcgatgga aatcgattat
    10441 tcccgccctt ggcgccgggc tcgctatgcc gacctgctgg ccgagcatgc aggctgccgc
    10501 atcgatgata tgccgtcggt acgggcaaaa gcccggcagc tgggcatccc tgaaaccaat
    10561 atggacgatg cggtggtcat caacgcggtt tttgaggaaa cagttgaatc gcatctgatt
    10621 aatccgacct ttgtgatgga ttatccggca gcgatttgtc cgctgaccaa gcaaagcaag
    10681 gctgatccgc gctatgccga gcggttcgag ctgtttatcg gcacgatgga gctggcgaat
    10741 gcctatacag aactcaacga tccggacgtg caggagcgga atttccgaat gcagcttcgg
    10801 ggtctgtcgg aagaggaaag catggcgaag atggatgagg attttattca ggcgctcaaa
    10861 tacggaatgc cgccggccgg cggactgggc attgggattg accggctgat catgctgctg
    10921 accaacgcca ccagtatccg tgatgttgtc ctgtttcccc tgctcaaacc gctggccgtc
    10981 aaaacagccg acacggcaga aaatacggac taactattgc gctgcaggct ccagagcggc
    11041 tcttgaagac gcctcccaaa tcatggatga atgctcttgt gcctgcggtc attaattaat
    11101 aaggacagat ggcttgaagc aaatcagcgt gttactgtgt tttcggtatc ttcagcatcg
    11161 gcggattgtc ctgctgagca tcgccgccgt agcgttaagc tgtgcgctgc tgattgcaac
    11221 agacagcttg tttaccggct ttatcagcgc cgttgaaaac agcgtcggcc agcatttggg
    11281 ggatattatt ctcgaggcac cggccaatcg caccattacg gattatgaag tcctgattgc
    11341 atcgctgcag caggctgcct gcatccggtc ggcaacagcc gtcctcagca gtcaggggct
    11401 tctgctgtcg gcaccgggga aggtgcgagc ggtccgggtc tggggaatcg agctgccgcg
    11461 ccggctggag gtgacgccgc tgcaggagtc cctgctgttt caaaaggaca aatcggctca
    11521 gcaggtgagt tttgatgtgc cgggtgcagc agaaatgaca gggggcattg tcggcattgg
    11581 gattctgacc cggcccgacg aaatgaccga tgaatacgat atagaagcgg tcaaaggaat
    11641 gctcggacag ccgatgatgc tgacctgcgg tgcagcatcg tatgagcagg acagccaaga
    11701 ccgaaccaac gggcggccgc gcttttcacg ccgggtagtg cggtttgcgc tggcagatgt
    11761 gatgatgacc ggggttaatg actttgacga atcctttgtg atgctgccgc tggactatct
    11821 gtcccgacag ctgaatcccc aaagcccgcc ggcagccaat attatccata ttcgtctggc
    11881 cgacggtgcg gatgagcaag aggccatggc cgtcgtgcgc ggtctatgga atacctttgc
    11941 ccaaacgcga ttggagtggt ataatttggc ttccattgaa tcggcccggg caatgcaggc
    12001 acgtttgatt gcagaataca aaaagcagat gggggttctg ctgctgattt tcggtctggt
    12061 ctcaatgggc atcattctgc tggtgttctg tattttctac ctgattgtga tgacccgccg
    12121 caaggatatc ggaattttga aaagctgcgg cctgtccagc ggttctgtgg cggcgttgtt
    12181 tgtgctgttt ggaaccgtcg tggggcttat tggagcagca gccggcatcg gtctgggctg
    12241 gctgattatt gacaatatca atgcaattga acaggcggtt gcgtcggttt tcggcatcaa
    12301 gctgtggaag gccagcacct acctgttcag ccggattccc aatacaatgc actggggctc
    12361 cgtcgtttgg attaccgcct gcggcatcgg agcggcagcg gctgggtcgc tgattccggc
    12421 ggttgctgcc gcccgcatca ggccggtaga aatcctgcaa tatgaataaa caataagccg
    12481 gtttctgcac ggctggactg cattcgagcg gaaagcagaa aaagaaatga ttaaatggct
    12541 tctggcatgg cggtatttta ttgggcgtcc gatttcgatt ctggcggtcg ctgcggtaac
    12601 gctgtgcgtt ttcatcgttg tggttgttat gacggtgatg aacggtttgg ttgaagattt
    12661 taagcagaaa aaccacagtt atgccggcga ctgtgtgatt gaatccgatt cactgacggg
    12721 ctttgcttat tatgaggagt ttctggagac cctcggcagg cagcctttca tccatgcggc
    12781 atcccctgtc gtcaaaggag tgggactgct cacaatgccg ggggccgact ggaacatcgg
    12841 cattgaaatt cacggtattg atccggtcct gcattcgcag gcaaccggtt ttgctgacac
    12901 cctttactat cacaaagatg ctccggctga tgcttttcgg ccgtcttatt taccagaaac
    12961 cgccggctgc attgtcggca tcgatatcat gagtcctcgc cgtcaatccg atggtggcta
    13021 ctatcatccc gatacccctg ttgcattgga gctgattatc agtgcctttc cgcttaatct
    13081 gcggggcgga ctggcacgag ccggcacaga ctttatcagc accaaatcgt tttattacag
    13141 cgatgacagc cacagcgggc tggtcaaggt tgatggagcg gtggtctatg tgccgctggc
    13201 cgaggcacag attttgtgcg gtatggatgc cccgctgaag cgtgccagtt ccattcatat
    13261 tcgctttgct gcgggaacat cgcctcttga tgccctcgag aaagtccgga cgctgtggag
    13321 cgagcatcaa tcccgctacg gcagccagcc cgggggcgaa ctgctgacgc acgtccgtgt
    13381 ccaaagctgg caggaaaacc gccgcagcat cattgcccca atggaaaaag agcagaccat
    13441 gatgatcatg ctttttatca tgctgggtct tattactgtg tttatcgtcc ttgtggtgtt
    13501 gtatatgatt atcagtcata aaagcaaaga tatcggcatt ttgaaaagca tcggcacttc
    13561 ggctgcggaa atgctggggg tatttcttct ttattcggtc tttatcggct tgataggtgc
    13621 cggtttaggc agtttgggcg ggtggttttt cttgtcaaaa ataaatccgc tggaagattg
    13681 gcttttcgag cattatcagt ggcagctgtg ggaccgtgcg gtatatgcta tcggcgatat
    13741 cccccatcag gtcgatgcgg atgttctggc ggctgtcatc ggggcggccg ttttatcgtg
    13801 tctgctcgga gccatcatcc ccagctatca ggccgcccgg aaaaaaccgg cacagagctt
    13861 acaagttaat cagctgtaga aatggattat ggaaataatt cttaaagcaa cgaatctgca
    13921 taaaacctac cgaatgggga aagtccgcct ggaggtattg cgcgggatta atcttgaaat
    13981 tccgcgggga caatttgcgg ccgttatcgg ggcaagcggc agcggaaaaa gcacacttct
    14041 gcatattctt ggggcattgg ataagcccga cagcggctcc gttcattacg aaaaccagcg
    14101 tctggaccgg atgcgatggt cccagttaaa tcattttcgc aaccgtacag taggttttgt
    14161 ttttcagttt taccatcttc tggacgaatt aaccgtgctg gaaaatgtga tgaccccggc
    14221 gatggtttcg gcgggaattt tcaggtggat aaagacaaaa gcgagcatcc gcaaacgggc
    14281 atatgagctt ttggagcagt ttggtcttaa agaccggctt gaccacaaac cctatgaatt
    14341 gtccggcgga gaacggcagc gggtcgcaat tgcccgtgca ttaataaacc agccgcccct
    14401 gcttttagcc gatgagccga caggaaatct tgactctcgt accggacatg gtattttaga
    14461 taccttagcc gaattaaacc gacaaggaca gacgataatt atggttacac acgatgagcg
    14521 cattgccgcc cgcgcaagcc ggattatccg cctggtcgat ggaaaagtca aagaggttgc
    14581 ggtaaatcag tagaactgct taacaaatgg agaatttatg ggaccgaaaa tatggatcaa
    14641 taactgttta gttgaccagg cggaagcaaa ggtgtcggtt tttgaccatg cacttctgta
    14701 tggggacggc gtttttgagg gcattcgggt gtacggcggc cgcatttttg aatgcaaggc
    14761 acatctggac cgactttatg aatcagccaa agttataatg cttaaaatcc caatggaaat
    14821 ggagggatta acccgcgcaa tggaggacac agttgcggct aacgggatac gagagggtta
    14881 tattcgtctg attgttacgc gcgggattgg ggatctggga ttaaatcctt ttttgtgcaa
    14941 aaaagcacag attatcatta ttgccgacca aattcagctg tatcctgagg aattgtacga
    15001 caaagggata aaagtaatca gcgtttcaag tgttcgcaac catccgctga cttgtccgcc
    15061 gcaggtaaaa tcgcttaact acctcaacaa tgttttggcc aagatagaag cggtccaggc
    15121 gggcgccgat gaagggatta tgtataatca tctcggatat gttgcagaag caaccgggga
    15181 taatgtcttt attgttaaaa acggcattgt ttatactccg ccgattcagg ccggctcgtt
    15241 aggaggcatt acgcggcgtg tggttatccg tctggccaaa caagaaaaca tcaatgtagt
    15301 ggaaaccgac ttgacacggt ttgatctcta tgtagccgat gaaatgttct taacggggac
    15361 tgccgctgaa gtaatcggtg tggcagaaat ggacggccgg acaatcggtt caggcaatcc
    15421 gggaccgatt acgctgcggc tgaggaaaaa attctttgaa tacgcccatt cctgaaaatg
    15481 cggcttttct gggcagctgg ttgacggcag gtatagatgc cctttttgaa cctgttcgaa
    15541 atgaactggc agaagtacag gggctgattg ctgaacagat gcaatcgccg gaagaggaga
    15601 taaacagctg cctgcgtttt ctgcgccagc ggccgggcaa aatgcttcgt ccggccctgc
    15661 tgctgctgtg cgggaaaacc atagaagaac gtctaacaac ggagcataga gaccttgcgg
    15721 ccatgataga actggtgcat atggcgtccc tgctgcacga tgatgtgatt gattcagccg
    15781 acctccgtcg gggatttccc agcgccaatg ccttatgggg aaataccgca gcggttttgc
    15841 tcggtgatta tctgctctgt cgagcttttt ctctgggcat tttatgccgt cttgccgatg
    15901 ccgccaaact gttggggcaa accgctcaaa cgctgtgtac gggggaattg aagcagaatc
    15961 ttctcaaagg ccgtccgagt ctgaacgaac aggattatct tcaaatcatc gagtccaaaa
    16021 cagctgtttt atttgcatgc ggctgccggc tgggggcgat ggcttccggc gcttcggagg
    16081 cacaggaaaa ggcgctgaaa gacttcgggc ttcacttcgg aatggccttt cagatagccg
    16141 acgacttgca ggacatcctg tccacggctg cccaaaccgg caagacacag ggtattgaca
    16201 ggacacagga aaaatggacg cttccggtca tccgctggat tagtcaggac ggctcagacc
    16261 ggcaggcaag gattaaccag tggagcagcg caacggatgt ccgcgtacta atcaatcaaa
    16321 tgcgttcgga cggcagcatc gattacgcct ttcagcaggc ccggcaggag gttcagcagg
    16381 ccaaacagca attggacagt ctgccggata cgccggcccg ccagtcgctg aagatgctgg
    16441 cagaccatat tacagaaccg atacgcacag ttttgaatgc ttagcgtctt tcggcccgcg
    16501 cataaaaaaa acaaggccgg ctgcaaaacc ggccttgtct tttcttgtct tcaaaacttc
    16561 ttagaatgtc agggcgtagg aaatcattcc ccacagctcg tcttcattgt tgacagtatc
    16621 ttcgaaggag ttctggaagt aaacacccgg aacaacctta ccgccggtca gcgggcaggt
    16681 aaaggaggtc tttaagctcc agaccatatg cgaccagtcg ttatcaacat ccggaccgcc
    16741 ggtcccatca ttgtacacaa tatcccagcc gaacatcatc ggcagatcag gggcctgttc
    16801 gagcttgaag ttatatccaa aacccatcgc ataaatggtt ccggagaaat ctttatttaa
    16861 tccttcaccc ttggaatccc acatctgata gacagcgccg cggggtacca aaccggagtc
    16921 gcccagcaga gacggcatcg caaactctgc aaacacttcc tgcacatctt tatcctttgt
    16981 aggcatgtca ataaaatcat agtagcgcca gccgactgtg tagtccgtct tccacgggca
    17041 gctttcggca taggtgccgc tgtaggccag cgtgtagcgg tattcggtcg cattgacccg
    17101 gctcatctgg tcatttccgc cggtgcctgc ataggatgcc catgcattgg cgctgaagcc
    17161 gttgtcaaag ccgaaattca cgctcggctg ccaggcagag gcatcatcca gcatgtcgaa
    17221 acctctccag atgtacttgc tggcatacgt taaatcaatg gttgtcttca catccgcggc
    17281 gcttgctaca gcagcagcac accccaccag tacaatccct accaacagtt tcttcatttt
    17341 tgatctcctt aacaaaaatt ggttttcgac tctacttcac taattattat atctcgacta
    17401 ttccgaaaaa aaccttgatg ttttccaccc ttgcgccgtc aaatatactt aaaaaaatac
    17461 agacttcaat acctatttta gaaaaaaact agaaataatc cgaagcatca gaaccccggc
    17521 cgcaagagat aaaattgcgt tcttcggatt aaaacaaatc agatgctcgg ttccggatga
    17581 aacatctgtt tggcctgacc taccagataa aaactgccgg tgatgcagat taaatcttca
    17641 cgcgttacag cgctgcaggc cacgcgaatg gcttctttga gcgacaaagc cgtctggcac
    17701 attttcccgc acaattcggt gtacatttcc gccaggtcgg tcgggaacac cgctttaggc
    17761 gaattgctgc gcgtaaaaat aaccttgtcg gctccatatt ggagctcagt gagcatcccg
    17821 cgaatatctt tgtcactatt gcagccgaaa atgatgacca ttgaatcata cggcacatgc
    17881 tgaccaatag cttgaatcag agcgcgaatg ctggcggcat tatgagccgc atccaccaga
    17941 atgcggggat cttcgctgat catttccata cggccggcca gccggacttc gctcaatccc
    18001 ataacggctt tctggtcgtc aattgtatag ccgcggtttt tcagctggtc cagcatagcc
    18061 aaagccaagc cgcagttgat ggcctgatga tctcccggca gcggcacacg caaatgctca
    18121 aaccggctgc tgggcgttgt caggcagatc cgcgtatgcg ggccgtattc gcgggaggat
    18181 tcgaaccgat acgaaaaatc aatatccttg ccggttacaa tcagcggcgc cttttgaaca
    18241 gcagcatatt tcttgagaac ccgcatggct gccgcatcct gcggaacagt aacgaccggc
    18301 acaccttttt tgattacgcc ggccttttct ttggcgatgc agtcaatcgt cggccccagc
    18361 agattttggt gatcgatgct gatggaggta atgccgacca attccggcat aatcacattg
    18421 gtgctgtcca aacgtccgcc aagtcccgtt tcaatgacgg ctaaatcgac ctgatgatcc
    18481 gcaaaataca taaacgccag agccgtcata atctcgaaga aagtcggaca ttcattggcc
    18541 tctgaaagct tctcgataac cggatagacg cgattcatca aatcgagcat ttctttgcgg
    18601 ctgatcatct gggaattcac cacaatccgc tcatgcagag tcatcacgtg aggcgaggta
    18661 tacaagccga ccttgtagcc gttggcttcc accattttgg ccagcattgt agcagtcgaa
    18721 cccttgcctt ttgtgccggc gatatgaacc agttttgttt tttgatgcgg attgccaagc
    18781 cctttgagca gcttgttcat ccgttcaagg tcaaaagcag ttacattata gcgcagtttc
    18841 tgctgcttct catagtccgt cttatcgaac agatactgaa tggcctgttc atacgtatga
    18901 aacgccttcg caggacgttt gacagtagcg acggaagcgg atgagggttt ctttttacgc
    18961 gtttctgaaa gagtagtctt ccctggtctt ttctttgttg ctgtcataga taatgtaatg
    19021 ttaacacaaa aattctaata agtcaatgaa gcataaacat ttcttgcgca aaaaaacaaa
    19081 aacgttcttt gtttatatac ataagtcctt attatagata caaaagcaca acgtgtgaga
    19141 taaattcaat tatagaaagc atgcctatca gatatactaa tatatgtatt aatattattg
    19201 atttttgttt ctgctttaac aaaagttggg ctcaattggc catactttat ttcaactata
    19261 aaatttcttg cattaacata tcctgactgt taacataatt ctctttattt tagacgtgaa
    19321 aacagagatt ttgtttaata tattctgtca aattatgaga ttcaaatatt cttagagctg
    19381 cacacatcca agatgctggg acagggtatt caatacatca agcttaaaaa acaaacttaa
    19441 aaatgagcga gagtaaacta tggccaaact gacagaactg gacaatctat gtattcaaac
    19501 aattcgtttt cttgctttgg atggcgtaca ggccgcaaaa tccggtcatc cgggaatgcc
    19561 gatggggatg gcagcggctg cgtatgcttt atggcagcgt catcttaaat tcgaccctgc
    19621 tcatcccgaa tggattaacc gcgaccggtt cgttctatcg gccggacacg gcagtatgct
    19681 tttatatgca ctgctgcact tattcggcta tgatttgccg ctggatgaac tcaaacggtt
    19741 tcgccagtgg ggcagcaaaa ctcccggcca tccggaatac ggtcatacac ccggcgttga
    19801 ggtaaccaca ggtccattgg ggcagggtat ttcgaatgcg gttggaatgg ccatagccca
    19861 aaaatatctg gccgcacgct ataacaaaga gacattcaat ctttttgact atcacatcta
    19921 tgtcatctgc ggcgacggct gtctgcaaga aggcatcagc ggggaagcgt cgagcctggc
    19981 cggccatctg gggctggata acttgattgt catctatgat gataaccata ttacaattga
    20041 cggcgaaaca agcctgtctt ttaccgaaaa tgtagcactg cggtatcgtg cctatggctg
    20101 gaacgtgctg gaaatcggcg gcgacgggca cgacattgat gccctcgacg gcgctttgga
    20161 gtttgccaag gcccagtccg aacggcccac gctgattaag tttcgctctc atatcggcta
    20221 cggcagcccc aattttcagg atacgcatac ggctcacgga gcaccgctgg gtgaagacga
    20281 aatcaaattg attaagaaaa atgccggctg ggatccagag cagaaattcg ttgttcccgc
    20341 cgaggttacc gcacataccg gcaaagcaaa agaaaccggt cggaaattgt atgccgatta
    20401 ccagaagctc tgggctgcct acgcccgggc atacccggaa ctggtcaaag aacttcaaga
    20461 tgccttgaac ggaaatgtcg tcattgatga gaaaaaactg cctgtttttg atccggcgac
    20521 gcctctggcc acccgtgcag cctcgggcaa gacactggat gccctgatgc cttcaatgcc
    20581 gctggtgctg ggcggctcgg cagacctgac accctcgaat aatacccgct ttaaaggggc
    20641 cgaggatttt cagaagacca accgcgcggg gcggtatatc cgatacgggg ttcgggaaca
    20701 tgctatggga gcaattatga acggcatagc cgtcagcaaa atccttcggc catacggcgg
    20761 cacatttatg gtatttgccg attatatgcg tccggctgtt cgtctggcgg caatgtccgg
    20821 ctatccgacg atttttgtct ttacccacga cagcatcgga gtcggtgaag atggtccaac
    20881 ccaccagccg gttgaaacgc tggcctcttt gcggattatg cccaacctgc tgacattccg
    20941 gccggctgat gccaacgaaa cagcattgat gtggaaatat attctggaac atcccgatat
    21001 gcccgcggca acagcactga cacgtcagaa tctgcccgtg ctggatccgg ccaaatatcc
    21061 gggcatgaag aacttcaaca aaggtgcgta cgtccttatc ggacaaacca aacccgatgt
    21121 gctgctgctg gccaccggtt cggaagtagc gctggcgtta gccgcccatg acaagcttgc
    21181 tgctgagggc atcaaagccc aagttgtttc gatgccctgc tggacgctgt ttgacaaaca
    21241 ggatgatgct tataaagaaa gtgttcttcc atcggcagtc aaagcccgtg taggagtaga
    21301 ggccggcgta gaaaacggct ggtggaagta tctgggcact tgcggtcagt ttgtcggcat
    21361 gcatacgttc ggagccagcg gaacttataa ggcctgcttc gaaaactttg gattcaccgt
    21421 ggatgcggtc gtcgcagcgg ctaagaaatc catcgcaaaa gcataacagc cgccggagtc
    21481 tgttgaaata caaggccttt gtttttatcc atcaaggcct tgttttttgc gctggatgcc
    21541 gtaaagtata agaaaagcag actcttagga gccaacaatg gaatggaaca aaatcaaacg
    21601 catcgccgtg atgaccggcg ggggggactg ccccgggctt aacgctgtca ttcgagccgt
    21661 aaccaaaagt gcaatcaacc ggtacggact ggaagtctgg ggcgtagaag atggctaccg
    21721 cggtctgatt gaagaccgta tcggtcttct aaactacaat gatgtcagta atatcctcac
    21781 gctgggcggc accattttgg gctcttgcaa ttcgtccaat ccgttttcat ttccggttcg
    21841 tcggggccag cgcaccattc acaaagatgt atccgatacc tgcattgcaa acctccgaaa
    21901 gcgggggatt gaggctttgg tctgcatcgg cggcgacggg acaatggctt cagcggcagc
    21961 atttgccaaa aagggactgc cgatcatcgg cgtacccaaa accattgaca acgatatctg
    22021 gggaaccgac attaccttcg gtttcaatac agcagtaact acagcagccg aagcgattga
    22081 caaggtccat accaccgcca gctcgcacca tcgggtgatg attgtggaag taatgggacg
    22141 ctatgccggc tggattgccc tgcatgccgg cgtggcaggc ggctctgatg tgattctgat
    22201 tcccgagatt ccttatcagc tggagaagat tgctcaatat gtattggccc gctccaaaaa
    22261 aggcaaacgc ttcagcatta tcaccgttgc cgaaggcgcc aaacccaaag gcggtaaagc
    22321 ggttgtcttc aaaaccatca aagatagtcc cgatccggtt cgtctaggcg gcatttcatt
    22381 tgtgctggcc gaacagctgg gggaattgac ggggctgtcc tgtcgggcgg tcaatctggg
    22441 ccatattcag cgcggcggat cgccgacccc cttcgaccgc gtgctggcaa ccaatttcgg
    22501 tcattatgca ctggagctgc tgatgaaagg acagatcaac cagcttgttg tctggacgga
    22561 aggacggctg ggcagtgtgc ctctggcccg cattgcgggt aaaatcaaaa ccgtccggcc
    22621 aaatgacccg cttatcgccg ctgccaaagc cgtcggcacg agcttcggcg tatagtaata
    22681 ccggcataaa gccggattca ataatttgct ggatttaggt caggataaaa cagttcgtca
    22741 gattgcagcc cgtctggcag accacaatcc tgcattgggg cctgtaacag tcagcgggac
    22801 atggggatcg tttgcgcctc tgctggctgc ctatctgctc aaaacactca aacgtccgat
    22861 tctttatatc cgaccgcata ttgacgatgc cgatgacgcc gccgacgact tgcaggtttt
    22921 catcgggctg cctgtccaga catttccagt ctgggaaagc cccgatgaaa ttaccgatgc
    22981 cgccgatgaa atccttgccc agagactgcg gctgtcattg tggatgaccc gacagcagcc
    23041 gcctgccgag ccgtttgttg tttcaacctg cgttcaagct ctcaaccagc cggttgctcg
    23101 cccgcaactg ctttttgaac tgggattgtc tttgtctgtc ggtcagacag tagagccgga
    23161 ccttgtaatt gggtggctgg ccgaccatgg cttcgaacgc accgacagcg tggatatgcc
    23221 cggtcagttt gcacagcgcg gcggcattat cgatatttat gctccggtct gtacagcagg
    23281 caacacaccc aatacagccg aagccatgcc ggttcgcata gagtttttcg gcgactgtat
    23341 cgacagcatt cgccgaattg atttagattc gcagctttcc catcaaatgc tgtcttcggt
    23401 gcagatcact gccccgccgc tgtcaattgc ccccgaaggc accgagctgt ttattaatct
    23461 gctgcctgcg gaaacgctga ttattctgga ggaacctgca gagattgcag aggtatccga
    23521 tgtattcttg agccggctgg aagatgtgcg cggactgtat ccgtggccag cggtttataa
    23581 agccattcag cgttttacga cactgcacat cagccgattc gccggttcgg catcgcctga
    23641 cttccagctg gatgtctgca gtgttcagca atttgagcag aaggccggct ctctctggaa
    23701 agaccaacag gccgttctgg cagagctgct tcgtgaagcc cggactcagc aggttctttt
    23761 ttattgcgaa aatcaggccg aaattcagcg cgtcagcgaa atcctgcagg aaaaatttac
    23821 tgttattccc gatacctttt tgctgctatc cggctttatt catcgcggct ttatgattcg
    23881 ctcgcttaac acccttgttg ttactcatca cgaggttttc ggtcaggtct ccatccgccg
    23941 ccgggtccag cggattcagg ggctttcgcc ggtcgatacc gcgctggact taaaaaaagg
    24001 cgactatgtt gttcatctga cctacggcat cggcaaatat tgcggcattc agatgatgga
    24061 aaaggccggc gccaaggccg aatatataac acttgaatat gccgacaaaa tccagataca
    24121 tgtgccgatt tccaatatgc atctggtaca gaaatttatc ggttcgagtc ctaaacggcc
    24181 gatgctcagc cgcatcggga caaaaaaatg gcagcggcag aaggaaaatg tcgcccgggg
    24241 tgttgcagaa ctggctgccg agctgctgga tgtgcaggcc cgccgccagc agatgggcgg
    24301 atttgccttc ggatccgata caatctggca gaaagaattt gaagaggcct ttgcctatct
    24361 ggaaacgccg gaccagacaa ccgctgccca gtgcatcaaa gacgatatgg cttccagtcg
    24421 gccgatggac cgtctgctgt gcggagacgt tggttacggc aaaaccgagc tggcgatgcg
    24481 ggcggccttc aaagccgttc aggccggaaa gcaggttgcc gttctggtac caacgacagt
    24541 tttatgtgtg cagcatggcc ggacctttcg gcagcggttt gctgactttc ctgttgccgt
    24601 tgagattctc aaccgcttcc aaacgcccaa agaaacccgc cggattcttt ccggctgccg
    24661 cagcggcacc gttgatatcc tcatcggcac gcaccgcctg ctgagcgatg atgttgcgtt
    24721 taaggatttg gggctgctga ttatcgatga ggaacagcgg tttggggtgg aacacaagga
    24781 gcggctgaag cgattccggg tcaatgttga tatcctgaca atgaccgcca cgccaatacc
    24841 gcgcacgctg catatgtcgc tgatcggtct gcgcgacatc agttcgctgt ctacgcctcc
    24901 gctggaccgg cgggctgttg caacaaaggt ctgccgattc agccccgagg ttattcgcaa
    24961 agggattttg tttgaaatca gccgcggcgg gcaggttttt ttcctataca acagggtaca
    25021 aaccatcgag cattttgccc atacgatacg ggccatcgtc aatgattcgt cggtgcgcat
    25081 tgatatcgcc cacggccaga tgcacaagca ccaattggaa gatgccatga ttcgctttat
    25141 caacggcgac accaatgtgc tgatttgctc gaccatcatc gaatccggta tcgacatccc
    25201 taacgccaat accatcttca tttatgacgc tgaccggttt gggcttgccc agcttcacca
    25261 gttgcggggg cgtgtcggcc ggtacaagca ccgtgcgtat gcctatctgc ttctgccgga
    25321 atcgcggccg attacgccga ttgcagcacg gcgtctgaaa gccattgaag aatactccca
    25381 gcttggcgcc ggtttccgca ttgcgttgcg cgatttggag attcggggcg ccggcagcat
    25441 tcttgggcct gaacagtccg gccatatcaa taccgtcggc tacgaactct attgccgcct
    25501 gctggccgaa gccgtcaagc ggctgaaaaa tgagccaatt gaaaaagaaa tccaaaccgt
    25561 tattgacctt ggttttgaga cttatattcc caaaagctat attccgtccg accgacagcg
    25621 attggaggta taccggcgca tcggtacagc acgaactgcc gaagacatcg accgcctgcg
    25681 taccgaactg cgggatttgt tcggcccagt gccggagcaa gtaaatatgc tgctggatac
    25741 cgcacaaatc cgtctctggg ccgaccgatg gcgtatccag agtatcattg tccaaaaacc
    25801 cgacattatt tttaccttct ttgacggggc ttccgggata gatctttttg cacgttattc
    25861 gggaacaata cgcatacccg acccgcgaac tgttcatatt cggcttgaaa aatcctattt
    25921 tgaaatcaaa acccttctgg ctttcctgcg aaagattctg cgacaataga cgatttttgt
    25981 aattcagaat tattgctcac tattcagaga taattttttc gcattctcgg ggctaaggcc
    26041 gatgcatcag ctgcctcttg agtaatgaaa caatatatac gaaagacggc aaacgagatg
    26101 gagacattgc cgagaaaata ttacattttg cggtggcaca gtcattccgg cggttagtcc
    26161 tctttgccgg gcagacagta cctatcaatt tatcttatac atcgccagtg ccatcctata
    26221 agatgcaggt attacaagcc cttaaaaaaa gacaaaaaac agctggaatc aacaagcatt
    26281 aaacatgcgg ccttgccaaa gcaaaaaatg ggattggccc ttttgaaaag cggcttttcg
    26341 gtatattggg gattaataaa atgcttgttt ttaccctatc aatatgatat aagccggttt
    26401 taataatcct aaactcactt tggagaaata gttatgtcat cggcccttga acagacagac
    26461 aaggttgtat ttgatttaat cagtcaagag gagcagcggc aggcggcttc gattcgaatg
    26521 ataccgtcgg aaaattatgt ttccaaagcg gtcatgcagg ccacaggaag ctgcctgacc
    26581 aataaatact cagagggcta tccgggcaaa cgatactatg aaggccagca gattaccgac
    26641 cagattgaaa cgctggcaca aaatcgagcc aaggcattat ttggagccca agcagccaat
    26701 gtacagccgc attccgggtc gccggccaat cttgcggcct attgtgcgct ggcccagccg
    26761 ggcgatacga ttatggggct tgcgctgccg cacggcggac atttaaccca cggctggaag
    26821 gtcagctata caggcaagtt tttcaattcg gtgcaatatg agctggatcc agccacaggc
    26881 cggctggatt acgacaagat agccgattta gcaaaaaaac accgtccgaa aatcctcatt
    26941 tccggctcca gtgcttatcc gcggattatt gacttttcgg ccttcgcaga aatcgccaac
    27001 agggttggcg cctatcatgt atcggatatg gcccacattg cgggtttggt tgccggaaaa
    27061 gtccatccca gtcctgttcc ttatgcagac atcgtaacaa caacaacgca taaaaccctg
    27121 cgcggacccc gcgggggaat gattttatgt agagcggaac acgaagcggc cgtcaacaag
    27181 gctgttttcc cgggcctgca gggagggccc catatgcata ccatttcggc tctggctgtg
    27241 gcgctgggcg aggcgatgac gcctgacttt gcggcctatg ccgcgcaaat tgttcgaaat
    27301 gccaagcggc tggccgaaaa acttctgggc tatggcttta accttgtttc cggcggaact
    27361 gataaccacc tgattctcat tgatttgcgc aacaaaggcc ttgccggaaa acccttagcc
    27421 aaagcgctgg atcgggctca tattgtctgc aattataact cggttcccgg cgatacggcc
    27481 ggccccttta atcccagcgg actgcgtctg ggaacgccgg ctataaccac gcgggggatg
    27541 aaggaagaac agatggacca gatagccgct tggattgata ccatcagccg gaatctggat
    27601 aacgatgacg taatcagcag gattggacaa gcggtaatag agttgtgccg gcagtttccg
    27661 ctgccggatc agtttgtacg ggtttgataa tccccgaaat ctatggcaga gcatccatta
    27721 aagctggctg cgataggcct gtcggacgaa gccgttgagc ttttggatat cgcgcaggaa
    27781 agcggcttat acgcgattgc ggctgtggga gatgagcgca acgaacgggc agaaaaaacc
    27841 gctcgccagt ttgattgtcc ggcttatacg gactaccggc agctgttggt gcaaacatcc
    27901 gcagacatcg tgttttttgg aggaccagtt taccaatgtg ccgagtttat ccggccggca
    27961 atggaagccc gctgtcatat tcttaaaagc tgcccgccgg cgctgcactt cgaacagctg
    28021 gcgcattttt accgcacagc cggcaaagaa catgtctgtt ttatcacagc tcagaatgga
    28081 cgtttcggac tgccctttga gcatattcgg aactatctat cccaaatagg gcaagacgat
    28141 cctcatcctt ggcatttgat ttcggcggtc tgccatgtac cgatggggcc gccggaacag
    28201 gagatgcgct ggctgtatga cccgacgatg gccggcgggg gtgtgctgct gcatcactgt
    28261 tatgatctga tagaccagct gattttgtgt tttgggctgc cgcagcgggt gtatgccctg
    28321 accatcaatc aggcccccga caaacagcag cggatgagct tgacggaaga tacggctgtt
    28381 gtggcaatgc agtttaccga tgcgctgatt gcgcagattt gcgccagccg gacattgggt
    28441 ccggcccgct ggcatctgcg ggtacacggc aaactgcagc atttaacggc aaccaaagaa
    28501 gaaatcatca tctgcgataa tctgggaaat ctgctggagc agaaggccta tccgaacgat
    28561 gaacgcccca gcaaaatgcg gatgctgcag catctggcag cagggctgaa gaatccgccc
    28621 gaacagccgc tttatcctga acacggtttt gatttgaata cgatggccgt gattgaagca
    28681 gcgtatttat cagcccgcac gggcatggcc gaagaacctg caaggattct taaattggct
    28741 gaaacaggaa cagccagcat tctatagcat cggacagaca tcctgtacag ggcattggtt
    28801 catcggcttg ctgatggagt cgaaaatgcc cggatggaac tatccattgc cctgcaccaa
    28861 ggccctgttc aacagataaa atgctgtcca tttttgcttg aaaagagcat tgattatggt
    28921 atatatgttc tttttaagta cttaaagaaa tgaatacaaa cgattattgt tccggctgca
    28981 gacaaaaaga gacctgcaga caggcatatg aaaagttagg ctgctatcaa ggtcctaaca
    29041 ttgcctggaa ggccatcagg gcatttgttg tgccgattgc gatttttgtc gcttcagcag
    29101 ccggtgcaaa tctgcttttc caaagatatt ttgaaggaac aagtttgaca ctggtttgtt
    29161 ttgtaggagc tgtcagcata acaacagcgg cagttttttt gttttaccgc cgacccaaaa
    29221 aagacatagc ggataaaagg taagcatcca tggccaagat acggggactt tttacatttc
    29281 gaggcggggt tcatccgccg gaaaacaaag aatacagcga acaatgcccg attgaggtgg
    29341 taccgacgcc caagcagacg gccgttctgc tcagccagca tctgggagca gtttgcaagc
    29401 cgctggtagc caagcgggat aaggtcactg ccggccaaat gatcggcaag acagatgcct
    29461 tcgtttcagc tccggttcat tctccggtca atggaactgt gcgggatatt acgctgctgc
    29521 cgcatccggt catcggccgc actatgggga ttatcatcga tgttgaccag cagattactg
    29581 ttcctaagca gcctgctgca ggacaatttg acaaaaactt tgatccgtcg gcgtactctg
    29641 tcgaagacat ctgccgggca gctcaggaag gcggcttggt tggtatgggc ggcgcaggct
    29701 ttcccacacg tgtcaaaata gagccgaatc cgtcgatgcc caaagaaacc ctgattatta
    29761 acggctgcga gtgcgaacct tttattacct gcgattatcg tctgatgctc gagtggacat
    29821 ggcaggtgct ggcagggata cggctggcgg ctaaagcggc aggcgtatca aaaatccgca
    29881 tcggcattga ggacaacaag ccccaggcca ttggcgtgat gacagaagca gtccagtcgc
    29941 tgggcggcac agaggatatc caagttgtgc cggttaaaac caaatatccg cagggcggcg
    30001 aacggcagct tattcaggca atattgaaga aaactgtccc caccggcagc attccgccgg
    30061 ccatcggcat ctgtgtgctg aatgtggcaa cctgtgcggc tctggcagag gctgtcgtac
    30121 tccatcagcc gctgacacac cgggtggtaa cggttaccgg acgggctatc agccgtccgg
    30181 gcaattatta tgtgcctatc ggcatcagtg ctgctgaact cattgattac tgcggcggac
    30241 tgaccgagca ggccgccaag gttgtgctgg gcgggccgat gatgggattt gcgattgcag
    30301 acctgtccca gccgattacc aaaaccagcg gtgcgctgac actgctgacc cgtcaggatg
    30361 ttacggaagc caaattctcc catcagcaga cgccgtgcat tcgctgcggg cgatgcttgg
    30421 cggtctgtcc ggagcggctg aacccaacca aaattgccca cgccgttaag catttccgga
    30481 tggatatcgc cgttcaaaac tacctgactg gatgcatcga gtgcggctgc tgcagttatg
    30541 tttgtccggc tcatatcgag ctgtcgggct atatcaagac tggaaaactt ctgatggcac
    30601 gggaaaaaaa acgtctgggg taacgttcga atacgctata ttgcagagga ttaaaaatca
    30661 atgttaagca aaattacggt ttcaccagcg ccgcatatca gccagaaacg gactacccgc
    30721 ggtgtgatgc tggatgtaat tatcggcctg tcgcctgcga tagcggcggg aattttcttt
    30781 ttccgaacgc aggcagcgct ggtgcttgcg gtcagcgttg ggtcgtgcat gtttttcgaa
    30841 tggatgtgca atgccatccg caagaaaccc aattcactgg gcgatttgag tgccgtggtg
    30901 acagggctga ttttggcgat gtcgctgccg ccggccattc cgctgtgggc ggttgttatc
    30961 ggaagcggat ttgccattat tatctgcaaa atgctgttcg gcggtttagg ttcgaatatc
    31021 tttaatccag caatggctgc aagggctttt ttgacggctt cattcggtct gctgatgacc
    31081 acctggacag tgccggcgcc gcttgatccg tcgatgccgc tgctgaaggc cgaaaacact
    31141 ctggcggtaa cgcaggccac ccctctggct tggagcaagg aagcgattaa gcataaagca
    31201 tctgcccaga cggccaatca gcagattggg gcggcttttt tcggaacggt aggcggatgc
    31261 ctgggcgaaa ccagctcgct ggcattgctt atcggaggtc tgtatcttct ggttcggcgg
    31321 acggttactt ggcatattcc cgtttgtgtg ctgttatcgg cggctgtgct ggcatcgcta
    31381 ttttattgtg tcaatccgga tcgctatgcg tctcccctgc tgcatctatt tggcggcggg
    31441 atgctgattt gcgccttttt tatcgctacc gatccggtta ccgcaccctt gaccgcaaaa
    31501 ggaatgtgta tctttgggct gggagttggg gcgctgatta tgctgatccg cattgtcggt
    31561 gaatatcccg aaggcgtcat gtatgcggtg ctgataatga atgccctttc gccgctgatt
    31621 gaccgtgcaa cgccgtgtat tccggcagga ggcaaaccca atgtccaata agcaaacgaa
    31681 aaatcggatt gtgtattttc tggaggaaag ctggctttta atggtctcat ccgttgtttt
    31741 cggatgtctg ctggcggttt tcaatgccct ctggagtccg cggattgaag ccaaccagca
    31801 atccaagttt gcccagctgg ctggaggcct gctgactggg gcagtcagct tcaaagaagc
    31861 tggaaccttc aacggcatta cggtaatgaa agccctgacg gccgacggct caccggcagg
    31921 atgggcgttt gtgtgcgagg gggcgggctt tgccgataaa atcaaactgg tggtagcagt
    31981 agatacggca tttgaaaaaa tggccggctt cggggtttta agcagcaatg aaaccccggg
    32041 cttcggcgat aagattacga ttaaacccga ggatggcggc ttttatcagc cccaatttat
    32101 tggtgcaccg gtggagacgc tgacattgac taaaaccggc gatgcatcca aaattgacag
    32161 tgagattgtc gccatcagcg gtgcaaccat cagcagtgaa gcggtggtat ctattttgaa
    32221 tgcatccctg ctgccggtta aagaggcgat gcgtcagcag ggcctgcttc cgtagaagca
    32281 ccggctgaaa aactgaacac tgattgtccg ctggaagttg atttcgataa caaagaaaaa
    32341 aaataaagag gacctgtaaa tgtccagtaa tgcttcgata ggaaagactt ttttggcggg
    32401 gatttgggac agtaatcccg tgctccggct gatgctgggg atgtgtccaa cgctggcagt
    32461 tacggcagcg gtcaagccgg ctgtgacgat gggcttgagc gttgtgttcg tgctgttctg
    32521 ctcgaatgcg gtagtcagcc tgatgcggaa tctgctcaag ccccacttgc ggattttgat
    32581 gtttaccttg acgatcgctg catttgtgac cattgctgat ttggcattga aggcctatct
    32641 gccggatatg agtgcagcgc tgggacctta tatccccctg atcattgtca actgcatcat
    32701 catcggccgg gccgaagcct gtgccagcaa aaacggcctg ctggtcagca ttgtcgatgc
    32761 ggttggaatg ggcctgggct ttacgctggc gctgtgcctg ctggcatcga ttcgcgaagt
    32821 gctggcaacc ggcaaatggt ttgatatacc ggtgatgccg gcggcgtttg tcccgtgggc
    32881 ggcgatgggg atgccggtcg gggcgtttgt cacgctggga ctgattctgg caggcgtcaa
    32941 cctgataacg aaaaaaaaca aggaaaaaaa ctgaaaatca ggcgaaggtt gccaacttta
    33001 tagaaagata aaccaatgga tactgcacaa acactgctgc tgggctttat cgggattgtg
    33061 attgtcaaca atctggtgtt tactaagttc ttaggcattt gtccgtatct gggtgtttcg
    33121 gggcggattg atatggcctt cgggatggga ctggcggtaa cctttgtggt gacgctggcc
    33181 ggcacgctga catggattat tgaccatagc tttcttatcg gcacgccgct ggaagtgatg
    33241 cgctatctat gctttattat ggtaattgcc ggagcggtgc agctggtcga gatgtatctg
    33301 aagaaattct ttccgccttt gtatgaaagc ttcggtattt ttctgccact gattaccacc
    33361 aactgcgcaa ttctgggaat gtgtttattt atcaacctgt gggctatcga cagtctgctg
    33421 atggccgccg tactcagtgc cggagccggc attggtttta cactggccat ctgcattatg
    33481 gccggaatcc gtgagaatct tcagctggcc gatgtgccgc cgtgcctgcg cggggcgccg
    33541 attacgctga ttacagcggg actgctgact ttggcattta tggggtttgc gggaatgata
    33601 aagggcagct gacaatggat aacattcgac tgacagccgc agccttcagg ggttgtccgt
    33661 caaaaatctt tgataacata ttagacagga atccacgatg ctgaatatcc tacttgccgg
    33721 aataatcaat aacgcctggc cggcagcagc gctggcgctg gggttgtcaa ccgtgtttgc
    33781 agcggtgctg ctgattgcca gtatcaagct gaaggtacag gaagatccaa aggtggagca
    33841 gattcacaag gccctgccga atatcgactg cggggcatgc ggttttgccg gctgtgcggc
    33901 gtatgcgaag gccgtagcgg ccaatcccga actgctgggc aagtgcgctc ccgggggcag
    33961 cgatacagcc aatgcagtag cggccattct gaatcttcag ctgtccggca gcggcgctcc
    34021 gagacggccg attattcatt gtcgggcctg tcagcaggat aaaacttatt ttgccaaata
    34081 cgagggtatc atcggctgca ccagcgccaa cgcccagccg aatgtgcagg cctgtgcgtt
    34141 cgggtgtctg ggcttcggcg gctgcgtccg ccgctgcaaa tttgacgccc tgcatattat
    34201 tgacgggctg gcagtggtgg attatgaaaa gtgcaccggc tgcggagctt gcgcagcagg
    34261 atgcccccgc gggctgattg aaatggtgcc gttcacgcat ccgatcatga tgaccgtagc
    34321 ctgcagcagc agggaaaacg gaaaaaatac gcgtgccttt tgcaaagtcg gctgcatcgg
    34381 ctgcggactg tgcgctaagc agagcgactt atttgcagtt tccgacaatc tggcacgaat
    34441 cgattatgcc cgttatgccc catccgaagc agcacaggca gcaatggaca aatgccctac
    34501 cggggcgatt gtctatcgcg gcacaaatgc gcccgtgcct cgccaagccg gttaaatatc
    34561 aacagcataa aaaacgacag ccgattgcaa ttttgtcaag catcttcaat cgagcagata
    34621 acggcctgca ccggcagttt tctcaaacac cggtttttct ccgcggggga tgagaatagc
    34681 ttcaacatcg gggagggatt caatgaacgg catccctttt ttatcgccca agacacttac
    34741 agctgttgaa agagcgtcgg cctgcatggc tgttggggcg atgatggtta cgctggagag
    34801 ggtttgagcg ctgtcggcgg tggtggggtc gatgatgtgg ctgtgttttt ggccggcgat
    34861 aatagtaaaa cgccggtagt caccgctggt ggcaacggcc cggttgtcca atttcagctt
    34921 ggcaaggata tttttgtcgc tggccggatc ctgcagacca ataagccaat gtggggcccc
    34981 atcggcaggt gtgccgaagc agcgaatatt gccgccgata tcaaccattc ctccgcgcag
    35041 gccggctttt tgaagaattt caacggcctt atcaaccgca tagcccttgg caatcccgcc
    35101 taaatcgagg aacattccct ctttagtgaa acgcactgtg cggttttgcg catctaaaat
    35161 aaggttgccg taacccaccc gttccttagc cgcctctaac tgctggggcg cgggggcagc
    35221 gccggttacc ctggcctgtc tccatatctg cacgaccggt ccgacggtaa tatcaaacgc
    35281 cccttctgaa agccgactgt attcaaccgc agcagccagc acttcaaaca attctgcatc
    35341 cacctctacg ggcgattcaa acgcctccct gttgaccttc gacaggaggg agttggggtc
    35401 gtaatcgctc atccgctttt caatgtcgta tattttctca aaggcggctt ctatcgcttt
    35461 tagaccatcc gatggtcttt ctgtgacaac aacaatgcgg gcgatggttc ccatcgtcat
    35521 ccgccagccg ctgccaatcg ctacagggcg gtccttgaac gctgcccaca aaagagcggc
    35581 agccgcaaat agtccgataa cagcttgaac tgatttcctt gttcgttctt gaagcattaa
    35641 aaattcctga atttttatgt cggcttctgg tcagcaaatg tcgaagtaag tataataagt
    35701 ttatgattca taaacaaata caaagcataa aggatattat aagtcggcag tttttgccgt
    35761 atgtgcgcaa gcccgcccgc tatatcggcg gcgagtttaa ccagatatgc aaagacctta
    35821 atcgctgcga tgttcggatt gcattgtgtt tccctgatat ttacgaaatt gggatgagct
    35881 atctgggttt ggaagtgctc tacagcatta ttaacaatct cgaaggtgca gccgcagaga
    35941 gggtctttgc gccatggttt gaagccgaag aagtcatgcg gagcaaaaag atacccttgt
    36001 ttacgcttga atcgcatgca ccggttcgcg acttcgatat catcggattc agcatgacca
    36061 atgagctttg ctacacgaat ctgctgaata tgctggatct ggcagggctg gagatacgag
    36121 cagaaaaccg gtccgacaac gatccgcttg tcatcgtcgg cggtcaggcg gccaactgtg
    36181 ccgaacctat cagcgctttt gccgatctgt ttgtgctggg ggagggggaa gaagctgccg
    36241 tagaactgat caagctgtat cgccgcattc aggccgccaa cggcacaaaa caggattatc
    36301 tggaagcgtg tgcacagcag ttctcgtatg tttatgtgcc gaagtttttc caggtgaatg
    36361 atgctcctga cagcagcagt tatgttaagc cgacaaaaga gggagtaagg gacaaattta
    36421 tgaatgcggt cgtacgggat ttcgagaatg ctcctattcc tgaaaaaccc atagtgccgt
    36481 ttgttcagac cgttcacgac cgggtcagca ttgagattat gcggggttgt cccggacact
    36541 gccgcttttg tcaggccagc tggtgccggc ggcctgtgcg ggtgcgaagt gtcgggcgaa
    36601 ttgttgaagc tgctaaaaag caatatgaag caacaggata tgacaccgtc agcttgctgt
    36661 ccctttcgac atccgattac ccgcagctgg aagaattggt cgaggccatg aatgctgtgt
    36721 ttagccctct gcatgtcggg ctgtcggtgc cgagtttgcg agtgcaaaag cagcttcagc
    36781 tcctgcccaa gatggtcgca tcggttcgca aaagcggtct gacaattgct gtcgaggcag
    36841 ccagcgaaaa actgcgcaag gtcatcggca aaccgatagc agatgaggac ttgcttgcgg
    36901 cagttcgggc tgcctttgaa gccggttttt tgaaggtcaa actgtatttt atggtcggct
    36961 ttcccggcga gacggaagag gatattttga aaattgcaga cttatccaaa acgatttcag
    37021 cactgcgaaa agagaccgac ggcaaatggg cggatgtcaa cgcagcagtc agctggctgg
    37081 tgcccaaagc ccacacccct ttcggctggc tggggcagcg ggatcgtgct tatttcgaac
    37141 aggcccggcg gctgatttta gaccgcaagc ggcagctgaa tctgcggtat gtgaagttta
    37201 attttcattc aattgagcgc agtattctgg aatcggctgt tggacgcggc ggccggcggc
    37261 tgtgcgaggt catcgaaacg gcctggcgaa acggcgccaa atttgactta tgggatgagg
    37321 gatttaagtt cgccgtctgg caggcagcat ttgcgtcagc cggcatggat ttagagcaag
    37381 aggccgccca cgcgctgccc cccggcggca ccacaccgtg gggccatctg ggaggtccgg
    37441 atgaaagata tttgcttaag gaataccagc aagcgatgac tttggtgcaa agttaacatg
    37501 atgtgtaaag cgcacagtaa aaaaaatcgc gtttgtttcg tatcgctcaa agcctatccg
    37561 cttttcaatc cggatatcag cagtgtgttc ggcggggcgg aggtggattt gtatatgctg
    37621 gcaaccgaac tggcaaacga tgaacggttt gaagtgtcgt ttatcgtagg cgattacggg
    37681 cagccgccgc tcgaagtccg acaaggcgtt actttgataa aatcgctgga tgcaaagaaa
    37741 actaaaattc tgcacggctg gaagatctgg aaggcactgc ggcgggctga tgcggatgta
    37801 tatatgcagg aagcctgctc gctggggaca tttctgattg ccctgttctg caaaatccat
    37861 cgccgccgct ttgtctaccg caccgcatct acgcaagaag cagatggaac ataccggcac
    37921 gatcatccca ttgccggtcg gctttttctc tggtcgctta agaatgctgc tgc
//
LOCUS       DAOTBO010000018        37956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_872_length_37956_cov_5.718055, whole genome shotgun sequence.
ACCESSION   DAOTBO010000018 DAOTBO010000000
VERSION     DAOTBO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 37956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37956
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_37956_cov_5.718055"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..574)
                     /locus_tag="PLE88_03910"
     CDS             complement(5..574)
                     /locus_tag="PLE88_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75645.1"
                     /translation="MSGRISPVCSVFLSAVLILSGCAKKMDAVPPTPQTPSTAQSERP
                     ISDSTPAAVLDKALELWRTGQKEQAADIFLQINWETAAVFTQNSVFAVSEGQFKTLPR
                     RRRQELLQKALLESTAVRELTQYLVKQARQPGTDIEKYRRALAACGQRLSANDQLAII
                     QLVGKAVSEYAKKELLTFHKLKERAFDDR"
     gene            631..756
                     /locus_tag="PLE88_03915"
     CDS             631..756
                     /locus_tag="PLE88_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75646.1"
                     /translation="MNKSVSIPAVGMFNNKDADRYLMFHKKSLLNDFAAAFAAMD"
     gene            complement(1075..1554)
                     /locus_tag="PLE88_03920"
     CDS             complement(1075..1554)
                     /locus_tag="PLE88_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM75647.1"
                     /translation="MNLSIRKASAADRQAVIDIIQATDFFRPVEIEIACEVFDEAAAD
                     KPGNTYQSYTAQADGTVVGWICFGPTPCTLGTFDVYWIAVHPAMQRHHIGTRLLAFAE
                     AEIRRQNGRLIVIETSGSEKYLPTRYFYQRSGYVLAAEVKDFYAPGDAKWIFTKTIA"
     gene            complement(1561..2556)
                     /locus_tag="PLE88_03925"
     CDS             complement(1561..2556)
                     /locus_tag="PLE88_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOM75648.1"
                     /translation="MHNTFGDAGDELYESRAGVMDQVQAVAQALAALGVGFEILAVEN
                     LRHLTAILSGRRETVIVNLAEEFLDSIQEACYVPAVCRAFGKSCTGSDTPALLLAQNK
                     IFAKAVLLGAGLPCPQGIAVAAGQKPDWHRLPAGRYIAKPAFCDASEGISAESVFVLP
                     DERQAAEMLLDKLHRQFAQPVIVEQFIPARELNVSVIQHGGRTDVMPLAEIDFSAFSD
                     AQPRIVDYDAKWRKESFGYSHTPRRIPADIPPQTAGRIAQLAAAAFETLGCRDYARVD
                     FRLDQQLHPYILEVNPNPDISPDAGFAAAIAAGGLSYGDFIHAIITNAHHRQTES"
     gene            complement(2627..3649)
                     /locus_tag="PLE88_03930"
     CDS             complement(2627..3649)
                     /locus_tag="PLE88_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOM75649.1"
                     /translation="MKLLVGLVYDLRSEYLAQGYSQEDVAEFDSDATLILLQEAIERC
                     GYRTERIGNGKALAQKLAAGCRWDMVFNIAEGIGGRCRESYVPALLEMYGVPYTFSDP
                     LVCAMTLDKRMAKQWVALHGLATPPFAVVEAMEDIRQVHLNYPLFAKPLAEGTGKGVD
                     QNSKIDEPAALEAVCAQLLDRYRQPVLVEEFLPGREFTVGVLGTGQQAAVLGTMEIEM
                     ASKDHPLIYSYLNKEECETRIYYHPLLDDRALKEQVEQLALQSYRVLQCRDAGRVDIR
                     CDRTGRPCFIEINPLAGLHPQHSDLPMIAAQEGMSYDTLIGTILENAFARYARQKEHR
                     KAVPLL"
     gene            complement(3686..5059)
                     /locus_tag="PLE88_03935"
     CDS             complement(3686..5059)
                     /locus_tag="PLE88_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOM75650.1"
                     /translation="MEQHTYAVAAEVMEEQSSEPPSSMRAVQSPSSCPTETETPLCKK
                     LSQWNDWRWQIRNRIRTLDQLAQYVPLLSGRGELQKVIDKYPMAITPYYASIIRRADM
                     SDPVFRMSVPNIQELFDSPCLTDDPLEEHEDMPVPGLVHRYRDRALLIATTMCSMYCR
                     HCTRKRIAGTRETAISARRMRQVQEYLFNHPEITDVIVSGGDPLTMSDAAIETVLSTL
                     RSVPSVQVIRIGTRVPVVLPMRITDQLVAMLKKYHPLWINTHFNHPQELTAEARAACA
                     KLADAGIPLGNQTVLLRGVNDDPQVIEQLCRGLISTRVRPYYIYQCDLVRGVEHFRTS
                     VRKGIEIMEYLRGRVSGIAIPTFVVDAPHGGGKIPVLPTYVVSMSPTHTVLRNYEGLL
                     VTYPEPGVDTVPAAGAAKAEPGVWELASGRGDVILPAHTQRMDRREKIHAKRAGAVQT
                     TGYLFDA"
     gene            complement(5094..5651)
                     /locus_tag="PLE88_03940"
     CDS             complement(5094..5651)
                     /locus_tag="PLE88_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM75651.1"
                     /translation="MKAALEVFSEYGIDAATIGDITQRADLGKGTFYRHFADKSEIVA
                     CLVEQAIDKLIGKLQIVGQATNVETALEDIINVHCRFFMEHPEEFILLFQGRLFLKLD
                     RQITEQMEGPFSRYLEVIENLLSPFMKEKKDIVRIRRLACAVAGFVFGFFSFAMIGME
                     AEEIETSIKPLRQSFVKSLSLFLAQ"
     gene            6025..6624
                     /locus_tag="PLE88_03945"
     CDS             6025..6624
                     /locus_tag="PLE88_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOM75652.1"
                     /translation="MAARMKKGLAIAIMCLLTASLALAAEDGGRRGRQNQPGGDRQNQ
                     PGGDRQRGGFNPDPGGMQQRMMEMMKTRLGASDDEWKIIEPRLSKVMELQRTTSNMGG
                     MMRGMGGMFGRGGRRPDVAGRQDALAAETDPVQKASDSLQETLDKTDASAEEIKAKLQ
                     AFRDAREKAQQELAKARQDLRDVLTLRQEAQLVLMGMLD"
     gene            6645..8360
                     /gene="gspE"
                     /locus_tag="PLE88_03950"
     CDS             6645..8360
                     /gene="gspE"
                     /locus_tag="PLE88_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOM75653.1"
                     /translation="MNPFLAVLQKNGLLSEPDIESVNQHLSQGEDLLTALLATGRLPE
                     EVILRHLAEAMQYPFYESLDQFESSKSFLARFPARILLERHIAPVPGSNGSVLTLICN
                     PFDLSGVDQLRLFTGSDYEIGLAPRGEISRYVKRYLGLGADTIQTMMDEADDKELQFL
                     TQTESEDVDLAEAAEGASIIRFVNQILTEAIELRATDVHIEPFEDELQVRYRIDGVLQ
                     AATIPPSVKRFQSAIISRIKILSHLDIAEKRVPQDGRIKVMVSGKEIDIRVSIIPMLH
                     GEAVVLRLLDCSSMLLGLDKIGMGPQDLAIFEEVIKQPHGIILVTGPTGSGKTTTLYA
                     ALSKINDVERKIITIEDPIEYQLRGVNQIQVSTKTGLTFARGLRSILRHDPDVILVGE
                     IRDLETAEIAVQASLTGHLVFSTLHTNDAPGALTRLVDMGMEAYLVSSSLAAVLAQRL
                     VRMICPDCREPLTEEEMEPLRKRYPTLVSQQLYRGRGCKACQQTGYRGRKGIFEQMVI
                     SEDIRALLMQNVSAREIRRQAMKEGMKTLRQDAKRFLDAGMTTIEEVLRVTKDDATAH
                     LTGVS"
     gene            8363..9571
                     /locus_tag="PLE88_03955"
     CDS             8363..9571
                     /locus_tag="PLE88_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM75654.1"
                     /translation="MPEFIYTATDRSGQVITDTITAPGRRDAIETLFARGLQPAKIEE
                     KTVSETKSVRRQGRVTRSDIETFTRELGNLLASGLSLSKSLKILSRESSKAAARQLWS
                     DIHDNVANGMGLAEALGQRPRYFSSIYVAMVQAGETGGFLELVLGQIADFRSREQELK
                     SRVQAALIYPIILMFLAVLILLFLLIYFIPRFSSIFAEFGGTLPPLTRGIVAVSQAVM
                     HYWFIIAGIIVLAVVTVRSFLQREEGQAAFERWILNTPIIGKLNARFAFVRFARMLGT
                     LLNAGVPLLAALRVAKEAIGYKTLSNTMNNAIEKVRKGVSLSQSLAECPQLFSGANIE
                     MISVAEESSRLGEELLRLAEVNEKELDRNLKTAVSFAEPLMLFAMAALVGTIVIGMLL
                     PIFNLQELIN"
     gene            9598..10035
                     /gene="gspG"
                     /locus_tag="PLE88_03960"
     CDS             9598..10035
                     /gene="gspG"
                     /locus_tag="PLE88_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOM75655.1"
                     /translation="MQKPRMPKAVGFTLIELLLVLVILAALATVVVPKFTKRSEQAKI
                     TAAQTDIANMEVVLDAFEIDTGRYPTTSEGIRALVEQPSNADGWKGPYLKRGVPKDPW
                     GNEYIYRQPGQHNEFGYDLSSPGPDGQPGTTDDIINWSLEERR"
     gene            10042..10560
                     /locus_tag="PLE88_03965"
     CDS             10042..10560
                     /locus_tag="PLE88_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75656.1"
                     /translation="MRGYSRHSVRSGAFTLLELILVMIILCTVLAIAAPSLRGFFSSR
                     KLNDIAEQMLIMTRYARLQAIFAGYPYRLNFDPNRREYWLSARRESVYEELKTSFGTR
                     HLIPTDIELAIDNVPQDGGIYYFEFTPQGYTKVCSIELRDSHRTRLALVCWSPSENFE
                     LVELRDDETLYN"
     gene            10538..10933
                     /locus_tag="PLE88_03970"
     CDS             10538..10933
                     /locus_tag="PLE88_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75657.1"
                     /translation="MMKHYTTNSVIQKQAGFTFMEILATMVLIGLILPAAMKGISVAV
                     ALASDSIRKQEALGLAENRLAEILLDEEWKSGAESGSFEPDAPGYRWTLETADRTEPG
                     LKQLDLTVSWEHRGYPKQIRLTTLVYNAE"
     gene            10947..11645
                     /locus_tag="PLE88_03975"
     CDS             10947..11645
                     /locus_tag="PLE88_03975"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOM75658.1"
                     /translation="MSKQAFTLLELIVAIALMDVIAVSLYASMHIGFTAKKNTQAAMI
                     PFRSVLPVFETIRNDLICAMPPTGILAGTFLGEDANNAAQKDADVLSFYTDSYQPATD
                     EIACNIIYVQYGLEEDPQRGGIVLKRKTIKNLLSTKTIIPDTEIIGRNIYGFDVKYYD
                     GYTWLDTWTSSTQNNTLPRGVRITLDLSPESRRSGQSPDQTRGTLFTRTFMLSSSSLA
                     AEQEAASSSSSARQ"
     gene            11642..12976
                     /locus_tag="PLE88_03980"
     CDS             11642..12976
                     /locus_tag="PLE88_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOM75659.1"
                     /translation="MKRNRIQLNRRIKRPGTVLIITIWIVTMLASLIIVFSHYIRVEA
                     LAASNHIALVQAEAAASAAVQYIIAMLASQETTEVNWSSNPYEGVQVGEGYFWVLRPN
                     LSEDRKYDFGLCAESGKININSASLETLLKLPSMTAELAASIIDWRDSNQELTAGGAE
                     NEYYLLLSNPYQCKNSPLETIEEVLLIKGATQQILYGEDTNRNGILDTNENDAEASPP
                     SDNANGRLDPGFFHYVTIHSYESNKDKDGNTRINVNDGRSQSQLADLLRQAVGGDYTR
                     YMNNIRQRRNYRSLIEVYFVSQMSYDSFNKIIDRLSTSNQEKASGLININTAPEEVLL
                     CLPGLEQSDVDAMIKYRTSRSETEESILWITKVLSREKAEAIGSYITTRTLQYSADIV
                     AASADGRAFRRYYVVIDTADGTPKVIYRQPLHTLGWPLDPAILLQLREGKGI"
     gene            13000..14370
                     /locus_tag="PLE88_03985"
     CDS             13000..14370
                     /locus_tag="PLE88_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75660.1"
                     /translation="MKQNRTMLGIVLYERSIAVCEAHLSGEGIRIRTSACYTMPEGQS
                     ADTLSSQSTAFRAFLKEHGFKAGKAVVGIPVKQMMTTLVKAPPVKNPELLHQTIQLNL
                     ERKIEADLTDIAFDYEVDADSSQSDILVTMVLKRTIAQIKELLESCRIHPVQITNTSL
                     GLDLPVSGTADCHIIEYPDSFELCLFRRGRLAAVQYIAKSGQQGLDAQTAQKIVRLVN
                     QTLWTASGQTDTASYTLWTFDAASDTSRSGAAVFGNFQCHRLKAAAGEGLSGLAAILA
                     EKVITGHPIPINYLNGRHSQAKPTLYKQWLHRGILGGAAVLVLIGGFLFNWHSDTKQI
                     ARLQEQLESMKSGVAAAQEMIDRVSTARAWFSDSPAFLEILRELTLSFPPNGNIWLTS
                     LAMDESHSQILTGRAVREQAVLDVAEELRKKTAFDDVKILYIRKMGKGTDIMTFAISL
                     RVREGI"
     gene            14372..14923
                     /locus_tag="PLE88_03990"
     CDS             14372..14923
                     /locus_tag="PLE88_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75661.1"
                     /translation="MALTNRERLIMILAAAAIVILAADRYILTPILDKREQTRQMRNS
                     LQTELEQSLAALERKKVLQQRWAQMQEAGLSYEVEAIENRVLRHLEETSGRSRFLLTS
                     IQPDRPTAGKQLGEIDFMVSGSGTMAAVTQFLWDIETSKLPLRIKSLQLGAADENGSQ
                     MTLQLKLSSIYLIKPESAKDTKS"
     gene            14920..15789
                     /locus_tag="PLE88_03995"
     CDS             14920..15789
                     /locus_tag="PLE88_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75662.1"
                     /translation="MTRMPLFIVLFLLSAVCIESLYAGPDIPPPPMEEPEAYGLPGEA
                     LTPPPAEVIEAIGAILQAQEPPAGTQPEKPVSSAETAEKTVQKAETAAAEPAPDSEQQ
                     IESESASAEPPAAQTEDLRPNSENGKELTGYDRYRIISQQNIFSRLRTPARPRSSRND
                     EKPAEEVKPVVIALYMLRGIAEQGSRKIAFVEDAISGQTLRAEIGSEVLNGTIRQIFI
                     DRVVFEENGQVRDIKIGDAFGKRESGRRDSGQTAPTAAPAAPAAPRAPAGSQSQEELL
                     RQMIERRNRELNR"
     gene            15808..17910
                     /gene="gspD"
                     /locus_tag="PLE88_04000"
     CDS             15808..17910
                     /gene="gspD"
                     /locus_tag="PLE88_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOM75663.1"
                     /translation="MNRKNYLIVIAAFMCLLTLPAPVGAQEAGPADPNGIVDLDGRFL
                     LNFQDALVEDILDYLSESVGLVIVAETFPTKRINLISKQPLSIDEVISLMNTIMKEQG
                     YAAVRMDRTLKMVPIENAKQMNLPVTTGSDPDKIVAGDDIVTHIIPIRYANAARLRED
                     ISALLPSYAVVTSNEASNSLIITDTTANIKRICKIVKAVDTQMAAVIDVRVFRLMYAD
                     ARNTAQLINEVFEQQSTQQTSRSDTNPFARMMEFGRGGPGGRDRGSQQSDQSSGGNAN
                     TRVIASADTQTNAVVVSGPSDVLEVIAKVVAELDANPRDERQLFVYKVKNAKAANLKD
                     KLNSLFQELQQLNQMGTSRTTTGRGGTARNVQTGLGGMFGGATGTTAASSDISDEVYI
                     EADEDTNSLLIMTSSRNYEKIKKIIEDLDYPVPQVLIKVLLAEVTTSHDIDLGVEFSH
                     LNLQPDGDSQEVKTIFPTNLASGDNTSGLTTKIIQSDLTATLRALEKIGRLNVLSRPY
                     ILTSNNQTATITVGQEVPFIRDTRMTETGQTINTIEYEDIGIILEVTPTINEDGLVIM
                     DVKPEISTTTAETVPISETVNAAVFAKRSSSSRVAVQNGQTIVIGGLMQDTETKSVSK
                     VPLVGDLPLVGELFKRTTVEKEKTELLIFLTPQVASNINDLKKISDHERSNSDVLKTT
                     DNPDLQKHLDKLESVYNK"
     gene            18107..18778
                     /locus_tag="PLE88_04005"
     CDS             18107..18778
                     /locus_tag="PLE88_04005"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HOM75664.1"
                     /translation="MLSNLPLASLLAANTLPLFGVLFLDWDAFAMVLLYWAENVILGF
                     YNIVKMILVKSPHPAGHLGKLFMIPFFLIHYGGFTAGHGLFVMVLFGRTISGGILPQQ
                     SWPCFLVFIQLLFHVIGEVIGMIPLNMVYAIAGLFISHGISFGYNFLYRGEYIKTNLR
                     TLMSEPYGRIVILHIAILFGGFLSKTLGSPVGILVLLVGLKTFIDVKLHLRQHCSTLQ
                     ATDNR"
     gene            18775..20004
                     /locus_tag="PLE88_04010"
     CDS             18775..20004
                     /locus_tag="PLE88_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOM75665.1"
                     /translation="MTINKQKPELLAPAGTLEKLKFACAYGADAVYFGMEFGSLRSFA
                     GNFTLKDAKDGLDYLHARGKKGYITLNIYPFSEEYDKLIRLAKQLDSIGADAFIISDV
                     GVLAELKKLHLHAAVHISTQANTTSWQAALAYKELGAKRINLARELSLEQIQEIQLHL
                     AGQVDTEVFIHGAVCFSYSGRCAISDYLAGARANRGECKHPCRWKYYLVEEKRPGQFL
                     PVFEDNRGLYLFNSKELALWEYVPALAAAGIGSFKIEGRMKSIHYLSSVVSFYRQVID
                     GKTFTQEEGLQLLNRIPNRGYSAGFIRGAIMPEDYSVDKSISEGTSVFVGNVIHSAAD
                     GCTIEVRNTIRSGDTLEVLTPDGQLSEITLSVPLKTVAGEYRDIATNGQFIQLPVFLP
                     EFSILRRLGDIKLQKSS"
     gene            20064..21455
                     /gene="murC"
                     /locus_tag="PLE88_04015"
     CDS             20064..21455
                     /gene="murC"
                     /locus_tag="PLE88_04015"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOM75666.1"
                     /translation="MNIKGKKYHFIGIGGIGTSGLAKVLMKNGAIVSGSDMSDSPVIS
                     QLRTRGAAVTLGHSPDNIPPDADEVVISAAITGANPELAGASERGLPIYKYAEMLGLL
                     FDRYTGIAIAGTHGKSTTSGWLAWILDRGGLKPNFIVGADIPQLGGSSGVGNGHFFVA
                     EACEYDRSFLNLHPTIAVVLNIEQDHLDYYRDEDEIVEAFGDFVEQVKPDGYIIANGQ
                     DKNVARILRRLKTGPKIITFGLESFCDYSARNLHLSGGSYEFDVYHNETLLGHIRIGL
                     PGRHNVLNALAVIAAAEAAGLNWSILQRELGDFAGIDRRLMLKAKIGGITILDDYAHH
                     PTEIRASLAAIREKYAPKRLWCLFQPHQYSRTRFLLDDFAESFKLADITIVPEIYFVR
                     DTAESRSKVSAETLVERITQHQCQALFIKDFAGIVEYLKQNVTDGDLVVTMGAGTIWK
                     VADEYIQWLGKHC"
     gene            21421..22299
                     /gene="murB"
                     /locus_tag="PLE88_04020"
     CDS             21421..22299
                     /gene="murB"
                     /locus_tag="PLE88_04020"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOM75667.1"
                     /translation="MNIFSGLESIVRKNCPLRDFTWYGLGGNAEYLIQPKTADQLREI
                     LNRCRENDMPVRVLGFGSNLLVSDEGVRGAVLKLDADAFCRYEFSGTTLTAGAGVNLN
                     KLVLESVRKGLGGLEALTGIPGSVGGAVRMNAGGNFGDIGSCVKSVTVMDKYGNQFEK
                     EKPELVFDYRWTNITAPVILSATIELTESDPDAMLKTVKEVWIYKKNSQPLNTRNAGC
                     IFKNPRGMSAGALIDRAGLKGTQLGGAVVSEKHANFIVTESGCTSSDVKQLIELIRTK
                     VKEVFDVDLELEIEIW"
     gene            22439..23413
                     /locus_tag="PLE88_04025"
     CDS             22439..23413
                     /locus_tag="PLE88_04025"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOM75668.1"
                     /translation="MQKELTQLKIAVLAGGIGSERSISLLSGQTVCSALKEAGFTVVL
                     SDITPDNTAILGDDSIDVFFPILHGQFGEDGHLQQLFEQRNLCFTGSGSQASRLAFDK
                     LLSKQVFFQAGLPIARHLVVHSSDTEADLKQLLPRLADKFVIKPVRQGSSVGVTITDD
                     LNIAAAKAMECLHQYGDCMVEEFIAGREITAGILNGTALPLIEIRSKAAFYDFKAKYE
                     SDETEYLFDTINDQSLVKKIQDTAAACFKELGCRHLSRVDFIVTNDGRPVILEINTLP
                     GFTSHSLLPMAARKAGISTPQLCRQIVEAAWKDYNNRSHRCSCRRSGS"
     gene            23476..24411
                     /locus_tag="PLE88_04030"
     CDS             23476..24411
                     /locus_tag="PLE88_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75669.1"
                     /translation="MTRKKAKSDTGSGGFFFQKKKRRKSGTAISWVTALKITLSIMFL
                     TLLAAGGAVGLIYLERYVKEAAAAQTPTGSLKLVNSPAWLNQDWIDALVKAAGGKRFE
                     LDDKSARQVARRLEQLSWLTDVRVQTTPEFLEVTAIYRRPIAIVSVSRTRKFYLDADM
                     VVLDYIPVSAIPVIEITGLAKTNVSEPGQIWLADDAKVAVELLNWLYLMDVHFQQEQK
                     LPKPLIDEIISVDVSNFAARKSRSAPNIVLNVKDGTRILWGAAWGQSDVYLEASEKIK
                     VARLYEYFIDHGYTLQGSAKNIELRWLEDSIPRPQ"
     gene            complement(24440..25612)
                     /locus_tag="PLE88_04035"
     CDS             complement(24440..25612)
                     /locus_tag="PLE88_04035"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOM75670.1"
                     /translation="MNITAHPVWTLIVLLTIEGLIVFIDSRPVGKKLFSILPSMFWIY
                     TIPMLAHTAGILPKQSEVYSWITAWVLPAALTLLLLSSDVSGIVRLGPVALGVMAIAA
                     IGILLGGPAAMLLLRPWLPDDAWKAIGPLCGSWIGGSFNMIAVKEAVGTPDELFGLII
                     LTDTLIAYGWMALLVSLAAHQSRFDCWNGSRIGLLNDLAARARQKSSPADSGNRIAGM
                     AAVAIIASAAAGISVWAGRLLPSVQGIINPAVWSIMLATLMGIGLSFTPARRLQTCGS
                     SRAGFLLLYLVLASLGAKTGLSYAGNLPVFLAVGVIWIVIHAACLLAAARLMKVPLAL
                     LAAASQACIGGTASAPVTAGIYHPQLAPVGLLMAVLGNIVGTFLGLLSAHLCRWLW"
     gene            complement(25609..26709)
                     /locus_tag="PLE88_04040"
     CDS             complement(25609..26709)
                     /locus_tag="PLE88_04040"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOM75671.1"
                     /translation="MTELLASYAVTESISRGRQIPEPPHPFRGAFERDRDRIIHSAAF
                     RRLEGKTQVFTTGLDDHYRTRLTHSIEVAQIGRTIARALNLNEALTEAICLAHDLGHP
                     PFGHTGEHILNDLMADYGGFEHNSQALRIVDFIECPYPDFRGLNLMFETRLGLVKHTS
                     PYDRPFRHSGWPVRPSLEGQIADIADRIAYNCHDLEDGLRSRLLDLSELTGLALYKLA
                     EKKIALDRIHDYVIRRTRLVKTIMDILASDVIETSRKKINEASIVRLEQVYEHPHPLI
                     GLSDSIHTALCQLETFLMQNMYLDPTIRQTAEKAQDWLAALFEDLCLCPDKMPHFYRQ
                     MIASQGLYRVVCDYIAGMTDRYCLQLALPLTQ"
     gene            complement(26706..27218)
                     /locus_tag="PLE88_04045"
     CDS             complement(26706..27218)
                     /locus_tag="PLE88_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOM75672.1"
                     /translation="MERKNLWAPWRIKYIQGLSHKEGCFLCDYLAAVEKDRDNCLLWR
                     TEQAFVVFNRFPYNNGHLLIAPNRHIATLDDASDEELLTLLTLVRDCQKALSLAIRPH
                     GFNVGMNFGRCAGAGLPGHLHIHVVPRWDGDTNFMHVCSDTDVISQGLYELYDQLVEI
                     SRTEHLPSRR"
     gene            complement(27239..28228)
                     /locus_tag="PLE88_04050"
     CDS             complement(27239..28228)
                     /locus_tag="PLE88_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75673.1"
                     /translation="MKNCWLWLILPLVLMTAGCRHTGQLPQDTWLLVSFEKGVPITYK
                     MVSERVTEIDLTTSDPSKKSRPQTMSEKLELVMSYTPMEVDPFGLTTLKAECVSAKVT
                     RSSFSGRKESQDAMETLPGKGFILKLSPTGEIADYSDLERITKELGKESFDKSATQNI
                     KNPDMLYDFIAMQWFLWDSAAAVPDPVRLTVGQTWQTKQLVPWPAPLYQPPARITTYT
                     LDHITAEPDQPRKAVIQCRYALSDEPMKDFVRPYDETSFQMRGLFGFLRNYKFKSLEG
                     TGTQIFDIDRGLIESEQQQYTLNVMAEFMLPLGDSLPVLTVSQTISLEYIPNP"
     gene            complement(28262..29209)
                     /locus_tag="PLE88_04055"
     CDS             complement(28262..29209)
                     /locus_tag="PLE88_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75674.1"
                     /translation="MSRNQGVILAVVAAGAAVLLSGCGPSAQLGLKLTPDSTARYKAT
                     TELIKDFRFDQPNLGKLREEQTKTWIEMGFTQTVESVDADGIAAVKVTINDLAVNVIN
                     KNEPKFAFNSADEKDKSSPLAKLLGQSYTIKLSPNGKAELLDAQAALASVSSGYEKQL
                     VTGLLDAKAVAERHQIAALPKEKAADISLKDSWTQVVPSPPGLLAPKTYKKVYTFTRL
                     DGHIATIDMVASETAEPAAEGTAASGSMGMFAKMFDNEDIYTGTMKLDTSTGTVLLSE
                     ETLVSRYVAQEMPENGDPAKGPDTLTMQFTNRIRLEKLD"
     gene            complement(29431..30960)
                     /locus_tag="PLE88_04060"
     CDS             complement(29431..30960)
                     /locus_tag="PLE88_04060"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOM75675.1"
                     /translation="MTDFGSSQAKEVSKVTVFLNDTKSYSPMIFGGFLEHFNRQIYGG
                     VYEPQSPLSNEKGFRLDVIEALKELKVPVVRWPGGCFVSGYHWKAGIGPNRIPVDDMA
                     WGVRESNAFGTDEFVELCRLAGWQPYICNNAGNGTIDEMVEWVEYCNTPETGAAYLRI
                     ANGHLAPFNVKLWSIGNENYGPWEIGHKTADQWAPMVREAAQRMKAVDAGIKLAAAAV
                     ASDEWVGRLLEAAGQYLDYISIHGYWLICNEKNQQPDYLTCVMYSNGPENQIVQTIQL
                     LEKYGYRGRIKIAFDEWNLRGWHHPGFPRREVSNYNDPHIIELVRARDKNLIPSQYTM
                     ADALFSASFFNACLRHSEDVGMANIAPLVNTRGPLYVYPGGIVKRTTFHTMAMYANLL
                     GSRVAKTEVRAGPLAGGGQSIPVLDAIGTVNEAQSVCTVALVNRHPLQEVICTIDFRG
                     APLPSGWLEAVVLAGDMPEAYNDIEHPHRVSPEKKQLLLDNGSVKLSPHSLTILKIPL
                     K"
     gene            31281..32948
                     /gene="ilvD"
                     /locus_tag="PLE88_04065"
     CDS             31281..32948
                     /gene="ilvD"
                     /locus_tag="PLE88_04065"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOM75676.1"
                     /translation="MNSDRIKKGFQRAPHRALLRACGLKTEDMNKPFIAVANSYCDIV
                     PGHVHLHEAAKAVKQAIRQAGGIPFEFNTIAVCDGIAMGHTGMKYSLASREIIADAVE
                     TMIRAHCFDGMVCIPNCDKVVPGMLMATVRLNIPTLFVSGGPMAAGKPKNGKATDLIT
                     IFEGVAQYNAGKITEADLTELECIGCPTQGSCSGMFTANSMNCLCEAVGIALPGNGTI
                     LAAALRRQELYKQAGRRIVEMVKEDLRPSSIITEQSLDNAFVLDMAMGGSTNTVLHTL
                     AVANEAKIPYDLDRINAISAKCPNICKVSPSSHWHIEDVDAAGGISAILKEISKTGLL
                     HTDCMTVSGVTLGQRIKDADIKNPDCIRPLEKAYSNTGGLVILRGNLAPNGCVVKAAG
                     VAPSMLKHSGPAVIFESQEEACEGILAGKVKPGDVVIIRYEGPKGGPGMQEMLSPTSY
                     IMGAGLGESVALITDGRFSGGTRGACIGHVSPEAAVGGPIALVKEGDIVEIDIPANKV
                     DVKLSDAELSQRRKSWKPRPPRITEGCLAKYAAMATSADTGAVLKWD"
     gene            complement(33056..33376)
                     /locus_tag="PLE88_04070"
     CDS             complement(33056..33376)
                     /locus_tag="PLE88_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75677.1"
                     /translation="MNKIIISIVVLTIAAGILFAFSGTPAVSHSESTAASAMASCCTQ
                     APAEGKCCADCQCTDCTCKAGSCGDSCKCETCKCGTCSCSDGKCGKGQNGCPKTGAGC
                     KKAA"
     gene            34500..35774
                     /locus_tag="PLE88_04075"
     CDS             34500..35774
                     /locus_tag="PLE88_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM75678.1"
                     /translation="MVRSCSSGVIRDRILRIAVYVILATLTGIVGGCITGKKTSTAGR
                     ADYGFIFLGDLHFDRPGHHDMDWVCSNYPGDVVQIQDYCRITEEHTPGLFGAISGTIK
                     ERPHPVFAVVQAGDFVEGLCGSYELQRIQFEEAKTFAEGYFKTVPFLISKGNHDITGP
                     AAQEAYRDVILPWIGGQLGRAVTQASYAVRRSNDLFIFFDAIRPDLDWLEETLRNSNA
                     RHTFFVTHKPVVPFDARANWHLFAEEGAAAQRERLLELLGRHRAIVLCGHLHAYSLLS
                     RKTAGGGFVQLAANSVVRQKEGAIEKYFVSKDAYGADLVSLEPSFSPVTLEVRKHNLE
                     TEKPEIGFFEYARTAGWCFIQCCPDKVVVEVYPGCSQTPWRVYDISLFGGNYTIEKAK
                     PDAGRRHQREDTGMPEITVEVEAGIGSFPGHK"
     gene            36160..36612
                     /locus_tag="PLE88_04080"
     CDS             36160..36612
                     /locus_tag="PLE88_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM75679.1"
                     /translation="MNNTWYSSGLRFECVGCGGCCAGPAEGYIWVSKPEIEFLARHLG
                     RSVKDFKKMYLKRIGLKYSIREDPLTKDCVFLEMVSNGCRSCAVYAVRPNQCRTWPFW
                     SFNLHSPEQWEMAAQRCPGINRGRLYTAEEIEAIKNQQTWWDNTIKKQ"
     gene            36660..37091
                     /locus_tag="PLE88_04085"
     CDS             36660..37091
                     /locus_tag="PLE88_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75680.1"
                     /translation="MHKQLIEEVAAIYRRLDEQTAPLAGGCTACGECCDFERFGHRLY
                     VTTPELLYFGYFARQPIQEMNGGICPYRISGKCSVYPYRFSGCRIFQCRQDSQQQQNT
                     ISEQTVRQLKTLCTHYSIPYHYVYLQAGLKMLRTGMLPEQL"
     gene            complement(37128..>37956)
                     /locus_tag="PLE88_04090"
     CDS             complement(37128..>37956)
                     /locus_tag="PLE88_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOM75681.1"
                     /translation="RTKIHLRSAPLDAGPYIKRCLFDAYKSVVLTSATLSCAPEGRDE
                     EGFAFFADRIGLDTYKALQVGSPFDYQRQVRLFIEADLPDPNEEAFLEPACEAIKKYL
                     LKSGGRAFVLFTSYSMLTETANRLRDWMTEQKMELLVQGGTLDRARMLARFKQGSRSV
                     LFGTDSFWQGVDVPGESLSNVIIVKLPFAVPNHPLIAGRIDMLKQRGENPFFAYQLPM
                     AVIKFKQGFGRLIRTRQDTGMVVVLDSRIVRKSYGRQFLDAIPQCRIDIVGLHDWAD"
BASE COUNT         9392 a         9670 c        10061 g         8833 t
ORIGIN      
        1 attattatcg gtcatcaaag gctcgttcct ttaatttatg aaaagtcaat aattccttct
       61 ttgcatattc agatacggcc tttccaacca gctgaatgat cgcaagctgg tcattggccg
      121 acagccgctg accgcaggca gcaagagcac gacggtattt ttcaatatcc gtcccgggct
      181 gcctggcttg tttgaccaaa tactgtgtca gctcccttac ggcggtcgat tccagcaacg
      241 ctttctgcag aagctcctga cggcgccgtc ggggcagtgt tttaaactgg ccttctgaaa
      301 cggcaaaaac agagttttgt gtaaagacag ccgctgtttc ccagtttatc tgcaaaaaga
      361 tatcggcggc ctgttccttc tgccccgtcc gccacaattc cagcgcctta tcgagcaccg
      421 cagccggcgt tgaatctgaa ataggccgct ccgattgagc tgtcgaggga gtttgcggag
      481 tcgggggcac agcatccatt tttttagcgc atcccgataa aatcagcact gccgaaagga
      541 atacactgca aaccggacta atcctgcctg acataatttc tcctttttta agaatgcgat
      601 tgcattttaa tcccaaatca gcaggaaaac atgaacaaat cggtatcaat acctgctgtt
      661 gggatgttta ataacaaaga cgcagaccga tacttaatgt ttcataaaaa atccctttta
      721 aatgattttg cggctgcatt tgctgcaatg gattaattcc gactttcaaa cctcctttac
      781 ccgcttaaaa aaacaggccg gtaaatcggc tctatactgg ctcaggtatg agcattaact
      841 ggcccgaaaa caagccgcag aatataatac tcctcctacc gtctccccaa agaggggggc
      901 gtgtgtgaga gattcctctg agaggatcat catggtctgc cggaaaaggt tcgcccagct
      961 gacgctctga cagccggcaa tcaatcggct tggttaatct cctcttgaaa ggattctgcc
     1021 aagagcaccg gcaccgcagc agccggcagg atgcggacat gaccggcgaa tgtattaggc
     1081 aatcgttttg gtgaaaatcc atttggcatc gccgggggca tagaagtcct tgacctcggc
     1141 ggccagcaca tagccgctgc gctggtagaa atagcgggtc ggcagatatt tctcggagcc
     1201 gctggtttca ataacaatca gccggccgtt ttggcggcga atctcagctt cggcaaaagc
     1261 cagcaggcgg gtgccgatat gatgccgctg catcgccgga tgcacagcaa tccagtatac
     1321 atcaaaagta cccagtgtac aaggagtcgg gccgaaacag atccagccga caactgttcc
     1381 atcggcttgg gctgtgtacg attgataggt attgccgggc ttatcggcag cggcttcatc
     1441 gaagacctca caggcaattt caatctcgac ggggcggaaa aaatctgtcg cctgaatgat
     1501 atcgatgacg gcctgacggt cggcggctga cgctttgcga atggacaggt tcatatctcc
     1561 tcacgattcg gtctgacgat ggtgtgcatt ggttataatt gcatggataa aatcgccata
     1621 cgacagaccg ccggcagcga tagctgcggc aaagccggca tcgggcgaaa tatccggatt
     1681 gggattaact tcgagaatat acggatgaag ctgctggtcc agacgaaaat ccacacgggc
     1741 ataatcgcgg cagcccagcg tctcaaacgc tgccgcagct agctgagcaa tgcggccggc
     1801 tgtctgcggc ggaatgtcgg ccggaatgcg gcggggtgta tggctgtagc cgaacgattc
     1861 tttgcgccac ttggcatcat aatccacaat gcggggctgg gcatccgaaa aggcactgaa
     1921 gtcgatttcg gcaaggggca tcacatctgt cctgccgccg tgctggataa ccgacacatt
     1981 taattcgcgg gccggtataa actgctcgac aatcaccggc tgggcaaatt gccgatgcag
     2041 cttgtccagc agcatttctg cggcctgccg ctcatccggc agaacaaaga ccgactcggc
     2101 gctgatgcct tcgctggcgt cgcagaaggc gggcttggcg atataccgcc ccgccggcag
     2161 ccggtgccag tcaggcttct gtccagcagc cacagcgatg ccctgcggac agggcagtcc
     2221 tgcccccagc aggacagctt ttgcaaaaat tttgttctgg gccagcagca gcgccggcgt
     2281 atcgctgccg gtgcagcttt tgccgaaagc ccggcagaca gccggcacat agcaggcctc
     2341 ctgaatggag tccagaaact cctctgccag attcacaatc actgtctccc tgcgcccgct
     2401 taaaatcgct gtcagatgcc gcaggttttc caccgccaga atctcaaatc cgacacccag
     2461 cgctgcaagc gcctgtgcca ccgcctgcac ctggtccatc acgccggcac ggctttcata
     2521 cagctcatcg cctgcatcgc caaaggtatt atgcaaaatc agaacagatt ccatcggatc
     2581 atcaacaaac cgccggttct aaatcaggtt cgttgtttac aggcttttac agcagcggca
     2641 ctgcctttct gtgttctttt tgccgggcat aacgcgcaaa ggcattttca agaatggttc
     2701 ctatcagcgt atcgtagctc atcccttcct gagctgcaat catcggcaga tcggaatgct
     2761 gcggatgcag gccggccagc ggattgattt cgataaagca gggccggccg gttcggtcgc
     2821 agcggatatc cacgcgtccg gcatcgcggc actggaggac acgatagctt tgcagggcca
     2881 gctgttcgac ctgctccttc aaggccctat cgtccagcag gggatgataa taaatgcggg
     2941 tttcgcactc ttccttgttc agataggaat agatgagggg gtggtctttg gaggccattt
     3001 caatttccat tgtccccagt acggccgcct gctgtccggt accgagcacg ccgacggtaa
     3061 actcgcggcc gggcagaaac tcttccacca aaaccggctg ccggtagcgg tccagcaatt
     3121 gggcacagac ggcctcgagg gccgccggct catcgatttt agaattctgg tccacccctt
     3181 taccggttcc ttcagctaaa ggcttggcaa acagcggata gttcagatgc acctgccgga
     3241 tatcctccat cgcttcgacc actgcaaacg ggggagtcgc cagtccgtgc agcgcaaccc
     3301 actgcttggc catgcgctta tccaacgtca tcgcacaaac cagcggatcg gagaaggtat
     3361 agggcacacc gtacatctcc agcagcgccg gcacatacga ttcgcggcag cgtccgccga
     3421 tgccttcggc aatattgaac accatatccc agcggcagcc ggccgccagc ttctgtgcca
     3481 atgccttgcc gttgccgatg cgctcggtac gatagccgca ccgctctatg gcttcctgca
     3541 gcagaatcag cgtggcatcg gaatcgaact cggcaacatc ttcctgtgaa tatccctgcg
     3601 ccagatattc gctgcgcagg tcatagacca atcctacaag gagcttcata acagtccgtc
     3661 ccggctgaaa ataacaggcc tcattttacg catcaaacaa ataaccggtg gtctgtacag
     3721 cccccgcccg cttggcatgg attttttccc gccggtccat tctctgggta tgcgccggca
     3781 gaatcacatc accccggccg gaagccagct cccagacgcc cggttcggct ttggccgcac
     3841 ccgccgccgg caccgtatca actcccggct ccggataggt caccaaaagc ccttcatagt
     3901 tgcgcaggac cgtatgcgtc ggactcatcg aaaccacata cgtcggcaga accggtatct
     3961 tgccgccccc atgcggggca tcgacaacaa atgtcggtat ggcaatgccg ctgacccgcc
     4021 cgcgcagata ttccataatc tcaatgcctt ttctaaccga ggtacggaaa tgctcaacgc
     4081 cgcggaccaa atcgcattga tagatataat acggccggac acgtgtgctg atcagaccgc
     4141 ggcaaagctg ctcaataacc tgcgggtcat cattgacgcc gcggagaaga accgtctggt
     4201 tgcctaaggg gatgccggcg tcggccagct tggcgcaggc agcgcgggcc tcagcggtca
     4261 attcctgcgg atggttaaag tgcgtattga tccaaagggg atggtatttt ttgagcatcg
     4321 caaccagctg gtctgtgatg cgcatcggca gaaccaccgg cacacgcgtt ccaatccgta
     4381 tgacctgcac cgaggggact gaccgcagtg tgctcaggac ggtttcaatg gccgcatcag
     4441 acattgtcag cgggtcgccg ccggaaacga taacatcagt aatttccgga tgattgaaca
     4501 gatattcctg tacttgccgc atccgccgag cagaaatagc tgtctcgcgt gttcctgcga
     4561 tccgcttgcg ggtgcagtgg cggcagtaca tcgaacacat tgttgtggca atcagcagtg
     4621 cccgatcacg gtagcggtgc accagccccg gaaccggcat atcttcgtgc tcttccagcg
     4681 ggtcatcggt cagacacggc gaatcgaaga gctcctgaat attcgggaca ctcatccgaa
     4741 agaccggatc ggacatatcc gcacgccgga tgatagaggc gtagtacggc gtaatggcca
     4801 tcgggtactt gtcgataacc ttctgcagct cgccccggcc ggacagcagc ggcacatact
     4861 gcgcaagctg atccagcgtt cggatgcggt tgcgtatctg ccatcgccag tcattccact
     4921 gggacagctt cttgcaaagg ggtgtttcgg tttcggttgg acaggaggag ggcgactgaa
     4981 ccgccctcat actactgggg ggttcagagg actgctcttc catgacttcc gctgcaacgg
     5041 cgtaagtgtg ttgctccatg gtttcagcgt tcctttcttt ttctatttat tggttattgt
     5101 gccagaaaca gagacaaact tttgacaaag ctttgtctga ggggtttgat gctggtttca
     5161 atttcctcag cttccatccc tatcatcgcg aaggaaaaga acccaaacac aaaacccgcg
     5221 accgcacacg cgagccgccg aatccgcacg atatcttttt tctctttcat aaagggcgac
     5281 agcaggtttt caattacctc cagataccgg ctgaaaggcc cttccatttg ttcggttatc
     5341 tgacgatcca gtttcaggaa taaacgcccc tgaaacaata gaataaactc ctccggatgc
     5401 tccataaaaa agcgacaatg gacattaata atatcctcaa gagcagtttc gacattggtt
     5461 gcctgcccta caatctggag ttttcctatc aacttatcaa ttgcctgctc aaccaaacag
     5521 gccacaattt ccgatttgtc ggcaaaatgg cgataaaatg tccctttgcc taaatcagct
     5581 cgctgagtta tatctcctat tgtcgccgca tcaatgccat attcactgaa aacctctaac
     5641 gcagctttca aaagctttgt ttttgtcttt tctacccgtt tttcagtcct ggaaacggct
     5701 tttttaaaga tctcaagccc atttccagaa tgttcagcaa gattatccat taccataaac
     5761 ggaccatata gtcacttgtg accaaatagt caatatcctt ttcgggaaaa tttactaaaa
     5821 tgttcacaaa atttttcaat taagggtaaa aacgaaaaaa agaccgatta atcttcaacg
     5881 aaaacaacag gtttatggta ttttaggcac atctgttttt tttgtaacga aacggcgggt
     5941 ccaatcacta ttagaatgcg tgcaggaatt catggcgcaa ttcctgcctg tactgttggg
     6001 ttatttgttt tacaggagaa atgtatggca gcaagaatga aaaaaggact cgcaatagcg
     6061 ataatgtgcc tcttgacggc atcgttggct ttagccgctg aagacggcgg gcgccgcggc
     6121 cgtcagaatc agcctggggg cgaccgtcag aaccagcctg gaggcgatcg tcagcgcgga
     6181 ggattcaatc cagatccggg cggaatgcag cagcgaatga tggagatgat gaaaacacgt
     6241 ctgggtgctt ccgatgatga atggaaaatt attgaacccc gcttgtccaa agtgatggag
     6301 cttcagcgga caacgagcaa tatgggtgga atgatgcggg gaatgggcgg catgttcggc
     6361 cgcggcggac gccggccgga tgtggccgga cgtcaggatg cgctggccgc tgaaaccgat
     6421 cccgtccaga aggcttctga cagtctgcag gaaactttgg acaagactga tgcctctgct
     6481 gaggaaatca aagccaaact gcaggcgttt cgtgatgccc gcgaaaaagc ccagcaggaa
     6541 cttgccaagg cccggcagga tctgcgtgat gtgctgacgc tgcgtcagga ggcccaactg
     6601 gtcttaatgg gtatgcttga ttaactttta ccggattgtt tcttatgaat ccattcttag
     6661 ccgttttgca gaaaaacgga cttctgtccg agccggatat cgaaagcgtt aatcagcacc
     6721 tttcgcaggg cgaagacctg ctgaccgcac tattggccac cggccggctg ccggaagagg
     6781 tgattctgcg tcatctggcc gaagcgatgc agtatccctt ctatgaaagt ctggaccagt
     6841 ttgagtcttc taaaagcttt ttggcccggt ttccggcgcg gattctgctc gaacggcaca
     6901 tcgcacctgt accgggcagc aacggcagtg tcctgacgct gatttgcaat ccgtttgact
     6961 tgtccggcgt tgatcagctt cgtctgttta caggcagcga ttatgagatt ggtctggcgc
     7021 cgcgggggga aatttcccgt tatgtcaaac gctatctggg tctgggcgcc gataccattc
     7081 aaaccatgat ggacgaagcg gatgataaag agctgcagtt cctgacgcag actgaaagcg
     7141 aagatgtcga tttggccgaa gctgccgaag gggcttccat catccgcttc gtcaatcaga
     7201 ttttgaccga agccatcgaa ttgcgggcca ccgacgtgca tatcgagccg ttcgaagacg
     7261 agctgcaggt gcgctaccgc atcgacggtg tcctgcaagc ggccaccatt ccgcctagcg
     7321 tcaaacgctt ccagtcggct attatctcgc gcatcaagat tctcagccat ctggatattg
     7381 ccgaaaagcg tgtcccgcag gatggccgca tcaaggtaat ggtcagcggc aaagaaatcg
     7441 acattcgcgt gtctattatc ccgatgctgc atggggaagc cgttgtgctg cgtttgctgg
     7501 attgctcttc gatgctgctg gggcttgaca aaatcggcat gggaccgcag gatttggcta
     7561 tttttgaaga agtcatcaag cagccccacg gcattattct ggtaaccggc ccgaccggca
     7621 gcggcaaaac gacaaccttg tatgcggcgc tgtcgaaaat caacgatgtt gagcggaaga
     7681 ttatcaccat cgaagatcca attgaatacc agctgcgggg tgtcaatcag atacaggttt
     7741 cgaccaaaac cggactgaca tttgcccgtg gtcttcggtc tattctgcgg catgaccccg
     7801 atgtgattct ggtgggtgaa atccgcgatt tggagaccgc cgaaatcgct gttcaggcgt
     7861 cgctgaccgg ccatttggtc ttcagcaccc tgcataccaa cgatgcgccg ggggccttga
     7921 cccgtctggt tgatatggga atggaggcct atttggtgtc atcgtcactg gcggcggttt
     7981 tggcacagcg gctggtgcgg atgatatgtc cggactgccg cgaaccgctg accgaagaag
     8041 aaatggagcc gctgcggaag cggtatccga cacttgtgtc ccagcagctg tatcgggggc
     8101 ggggctgcaa ggcctgtcag cagaccggct accgcggacg caagggcatc tttgagcaga
     8161 tggtcatcag cgaagatatc cgtgccctgc tgatgcagaa cgtcagcgcc cgcgagatcc
     8221 ggcggcaggc catgaaggaa ggaatgaaaa cactgcggca ggacgccaaa cggtttctgg
     8281 atgcgggtat gacaaccatc gaagaagtgc tgcgcgtcac caaggacgat gccacggctc
     8341 atttgacagg agttagctaa cgatgccgga attcatttac acagccaccg accgcagcgg
     8401 gcaggttatt accgacacga ttacagcgcc gggacggcgt gacgccatag agacgctttt
     8461 tgcacgcggt cttcagccgg ccaaaatcga agagaaaact gtatccgaaa ccaaatccgt
     8521 acggcgtcag ggtcgcgtga cgcgcagcga cattgaaaca ttcacccgtg aattgggcaa
     8581 ccttctggcg tcgggattgt cgctgagcaa atcccttaaa atattaagcc gcgaatcatc
     8641 caaagcggct gcccgccagc tctggtcgga tattcacgac aatgtcgcta acggaatggg
     8701 tctggccgaa gcacttgggc agcggccgcg ctatttttct tccatttatg tggcaatggt
     8761 tcaggccggc gaaaccggcg gttttctgga gctggtgctg gggcagatcg ccgatttccg
     8821 ttcgcgggag caggaactca aaagccgcgt gcaggcagcc ctgatttatc cgattatcct
     8881 gatgtttctg gcggtgttga ttctgctgtt cctgctgatt tactttatcc cgcggttttc
     8941 gtccattttt gccgaattcg gcggcacact accgcccctg acgcggggga ttgtcgccgt
     9001 cagtcaggca gtgatgcatt actggtttat tattgccggg atcatcgtgc tggcagtggt
     9061 gactgtccgg agctttctgc agcgggaaga aggacaggca gcatttgaac ggtggattct
     9121 gaacaccccc atcatcggga aattaaatgc ccggtttgcc tttgtgcggt ttgcccgaat
     9181 gctgggcacg ctgctgaatg cgggcgtgcc gctgctggca gcgctgcggg tagctaaaga
     9241 agccatcggc tacaaaacgc tttccaatac aatgaacaat gccattgaaa aggtgcgcaa
     9301 aggcgtttcg ctgtcacagt cgctggccga atgcccgcaa ctgttttccg gtgccaatat
     9361 cgaaatgatt tctgttgccg aagaaagttc gcggctgggc gaggagctac tgcggctggc
     9421 ggaagtcaat gaaaaggagc tggatcgcaa tcttaaaacg gcggtgtcgt ttgctgagcc
     9481 gctgatgctg tttgcaatgg cagcgctggt gggaaccatc gtcatcggaa tgctgctgcc
     9541 gatattcaac ctgcaggaat tgatcaatta acccttaatt tcaaggagtt tgaggttatg
     9601 cagaaaccac gaatgccaaa agcggtcgga tttaccttga ttgagctgct gctggtgctg
     9661 gtcattctgg cggcgctggc tactgtcgtc gtgcccaaat tcaccaagcg gagcgaacag
     9721 gccaaaatca cagctgccca aaccgacatt gccaatatgg aagtggtgct ggatgcattt
     9781 gaaatcgaca ccggccggta tccgaccacc agtgaaggca tccgggcgct ggttgagcag
     9841 ccgtccaacg ccgacggctg gaaaggacct tatctgaagc gcggtgtccc caaagacccg
     9901 tggggcaacg agtatatcta ccgtcagccc ggccagcaca acgaattcgg ctacgatttg
     9961 tcttcgccgg gcccagacgg tcagcccggc acgacagacg atatcatcaa ctggtctctg
    10021 gaagaacggc ggtagccggc aatgcggggc tacagcagac attctgttcg gtccggggcc
    10081 tttacgctgc tggaactgat tctggtgatg attattttat gcacggtatt ggccatagca
    10141 gcgccgtcgc tgcggggctt tttctcgtcc cgcaagctca acgacattgc tgaacagatg
    10201 ctgattatga cccggtatgc ccggcttcag gccatttttg cgggatatcc ctaccggctg
    10261 aacttcgatc ccaaccggcg ggaatactgg ctgtcggccc gccgggaaag cgtctatgaa
    10321 gaattgaaaa ccagcttcgg cacacggcat ctgattccaa ccgatattga attggcgatt
    10381 gacaatgtac ctcaggacgg cggaatttat tattttgagt ttaccccgca gggctacaca
    10441 aaagtctgtt ctattgaact gcgggacagc catcggacgc ggctggcgct ggtgtgctgg
    10501 agtccaagcg aaaattttga gctggttgag ctgcgcgatg atgaaacatt atacaactaa
    10561 ctctgttatt caaaagcagg ccggctttac ctttatggaa attctggcca cgatggtgct
    10621 gatcggtctg attctgccgg cggccatgaa aggcatttct gttgctgtgg cgctggcatc
    10681 cgacagcatc cgcaagcagg aagcactggg cttggccgaa aaccggctgg ctgagattct
    10741 tcttgacgag gagtggaaaa gcggcgccga aagcggcagc tttgagccgg atgcccccgg
    10801 ctaccgatgg acactcgaaa cagccgaccg gaccgaaccc ggccttaaac agctggacct
    10861 gaccgtgtcg tgggagcacc ggggctatcc caaacagata cggttgacaa cgctggtgta
    10921 caatgcggaa taagcatcta caatttgtgt caaaacaggc ctttacgctg ctggaattga
    10981 ttgtcgccat cgcactgatg gatgtcatcg ccgtatcgct gtatgccagt atgcatatcg
    11041 gttttacggc taaaaagaac acacaggcgg cgatgattcc cttccgctcc gtgctgcctg
    11101 tgtttgaaac catccgcaat gatttaatct gtgccatgcc gcccaccggc attctggccg
    11161 gaacttttct gggtgaagat gctaacaatg ccgcccagaa ggatgccgat gttctgagct
    11221 tttataccga cagctaccag cccgctaccg atgaaatcgc ctgcaatatc atctacgtcc
    11281 aatacggact ggaagaagac ccgcagcgcg gcggaattgt cctgaagcgc aaaacgatta
    11341 aaaacctgct gagcaccaaa acgattattc cagatacaga aatcatcggc cgcaatattt
    11401 acggctttga tgtcaaatat tatgacggct atacctggct ggatacgtgg acatccagca
    11461 cacagaacaa cacactgccc cgcggtgttc gcattacgct cgacctgtcg cccgagagca
    11521 gacggtcagg ccaaagcccc gatcaaacac gcggtacact ttttacgcga acctttatgc
    11581 tgtccagcag ctctttggcc gctgaacagg aggcagcttc ttcatcctca tctgcaagac
    11641 aatgaagcgc aatcggatac aactgaatag acgcatcaag cggccgggaa ccgtgctgat
    11701 tattaccatc tggattgtaa ccatgctggc cagtctgatt attgtctttt cgcactatat
    11761 ccgggtggag gccctggcgg catcgaacca tatcgcgctg gtacaggccg aagcggcggc
    11821 cagtgcggct gtacagtata tcatcgcgat gctggccagt caggaaacaa cggaagtcaa
    11881 ctggagcagc aatccgtatg agggtgtgca ggttggagag ggctatttct gggtactgcg
    11941 tccgaatctt tcggaagacc ggaaatatga cttcggcctg tgtgccgaat ccggcaaaat
    12001 caatatcaac tcggcctcgc tggaaacgct gttgaagctt ccgtcgatga cggcggaact
    12061 ggctgcatcg attattgatt ggcgcgacag caatcaggag ctgaccgccg gcggggcaga
    12121 aaatgaatat tacctgctac tgagcaatcc ttaccagtgc aagaattcgc cgctggaaac
    12181 cattgaagaa gtgctgctga ttaagggtgc cacccagcag attctgtacg gcgaagacac
    12241 caaccgcaac ggcatattgg ataccaatga aaacgatgct gaagcgtcgc ctccgtccga
    12301 caacgccaat gggcgtctgg atccgggatt ttttcattat gtaaccattc acagctatga
    12361 atccaataaa gataaggacg gcaatacccg catcaatgtc aatgacggga gaagtcagag
    12421 ccagctggcc gacttgctgc gtcaggccgt gggcggggat tacacgcggt atatgaacaa
    12481 tatccgccag cgccgcaatt accggagtct gatagaggtc tattttgtca gtcagatgag
    12541 ttacgacagc ttcaataaaa tcattgacag gctgagcaca agcaatcagg aaaaggcctc
    12601 cgggctgatt aatatcaaca cggctcccga agaagtgctg ctgtgtctgc ccgggctgga
    12661 gcagagcgat gtggatgcaa tgattaaata ccgcacctct cgaagtgaaa cggaagagag
    12721 catcctgtgg attactaaag ttctcagccg ggagaaagcc gaagccatcg gcagctacat
    12781 taccacgcgc acgcttcagt attcggcgga tatcgtggcc gccagtgccg acggacgcgc
    12841 ctttcgccgc tattatgtgg tgattgatac ggccgatgga acgccgaaag tcatttaccg
    12901 ccagccgctg catacgctgg gctggccgct ggatcctgcg attttattgc agctgcggga
    12961 aggcaaagga atctgaacca ttaaaaagaa agagtatcaa tgaagcagaa tcgaactatg
    13021 ctgggcattg tcctgtatga acggagcatc gctgtctgcg aggcgcatct gtccggcgag
    13081 ggaatccgca ttcgaacctc ggcctgctat acgatgccgg aaggacagtc tgctgatacg
    13141 ctgtcttcgc agagcacggc attcagggca tttttgaaag aacacggctt taaagccggc
    13201 aaggcggtgg tagggattcc tgtcaagcag atgatgacca cgctggtcaa ggcgccgccg
    13261 gtaaaaaatc cggagctgct tcatcagacc attcagctca atctcgagcg caagatcgag
    13321 gccgatctga cagacatcgc cttcgactac gaggtggatg cagacagcag ccaatcggat
    13381 attctggtga caatggttct caaacgcacg attgcccaaa ttaaagaact gctggaatcc
    13441 tgccggattc atcctgtaca gattaccaat acatcgcttg gattggatct gccggtttcc
    13501 ggtacggctg actgccatat tatcgaatat ccggactcat ttgagctgtg tcttttccgc
    13561 cgaggccgtc tggccgctgt ccagtacatc gccaaatccg gtcagcaggg actggatgcc
    13621 caaacagccc agaaaatagt ccgtttggta aaccagacgc tgtggacagc atccggtcag
    13681 accgataccg cttcttacac tctttggaca tttgatgccg ccagcgacac atcccgctcc
    13741 ggggcggctg tcttcgggaa cttccagtgc caccgtctaa aagcagctgc cggcgagggt
    13801 ttgagcgggc tggcggcgat tttggccgaa aaagtaatca ccggacatcc gataccgatc
    13861 aactatctta acggacggca tagtcaggcc aaaccgacac tttataagca gtggctgcat
    13921 cggggcattt tggggggggc agcggttttg gttctgatcg gcggcttttt gtttaactgg
    13981 cattcggaca ccaagcaaat tgcccgcctg caggagcagc tggagtcgat gaagagcggt
    14041 gttgccgcag cccaggaaat gatagaccgg gtttccaccg cacgggcgtg gttcagcgac
    14101 agccctgcct ttctggaaat cctgcgtgaa ttgacgctgt cgtttccgcc caacggcaat
    14161 atctggctga cctcgctggc aatggacgaa tcgcacagcc agattctgac cggccgggct
    14221 gttcgagagc aggccgtatt ggatgtggcc gaggaacttc gaaaaaagac ggcctttgat
    14281 gatgtcaaaa ttctttacat ccgaaaaatg ggaaaaggaa cagatattat gacctttgcc
    14341 atcagtcttc gtgttcggga gggcatctga tatggctctg acaaaccgtg aacgtctgat
    14401 tatgattttg gccgcagcgg ctattgtgat tcttgctgcc gaccggtata ttttgacgcc
    14461 gatactggat aagcgggaac agacgcggca gatgcgcaac tccctgcaga cagagctgga
    14521 gcagtcgctg gcagctctgg agcggaagaa agtgcttcag cagcggtggg cacagatgca
    14581 ggaggcgggg ctttcgtatg aggtggaagc catagaaaac cgcgttcttc ggcatctgga
    14641 agaaacatcc ggacggtcgc gctttctgct gacgtctatt cagccggatc ggcctactgc
    14701 cggcaagcaa ttaggggaga tagattttat ggtctcgggc agcggcacaa tggcggctgt
    14761 aacacagttt ctgtgggata ttgaaacatc caagctgccg ctgcggatta aatcgctcca
    14821 gctcggcgcc gccgatgaga acggctcgca gatgacactt caattaaagc tgtccagtat
    14881 ctacctgatt aagcctgaat ctgcaaagga tacaaaatca tgacacgaat gcccttattt
    14941 atagtcctat ttctgctgtc agctgtatgc atagaatcgc tgtacgccgg cccggatatc
    15001 ccccctccgc cgatggaaga accggaggcc tatggactgc cgggggaagc attaacgccc
    15061 ccgccggcag aagttatcga ggcaatagga gcgattctgc aggcacagga accgcctgcc
    15121 ggcacccagc cggaaaagcc ggtctcatca gccgagacag ccgaaaaaac tgtacaaaaa
    15181 gccgaaacag cagctgctga accggcaccc gattccgagc agcaaataga gtcggaatcg
    15241 gcatctgctg agccgccggc agcccaaaca gaggaccttc ggcctaattc tgaaaacggg
    15301 aaggaattaa ccggctacga ccgctaccga attatttcgc agcagaacat cttttcgcgg
    15361 ctgcgtacgc cggcacggcc tcggtcgtcc cgcaacgacg aaaaaccggc tgaagaagtc
    15421 aagccggtgg ttatagcgct gtatatgctg cgggggattg ccgaacaggg cagccgtaag
    15481 attgcctttg tcgaagatgc catcagcggc caaaccctcc gggcagaaat cggcagcgag
    15541 gtactgaatg gcacaattcg ccaaattttt attgaccgtg ttgtctttga agaaaacggg
    15601 caggttcggg atatcaaaat cggcgacgca ttcggcaagc gggaaagcgg aagacgtgat
    15661 tccggccaga cagctccgac ggcggctcca gctgcccctg cggcgccgag ggctccagcc
    15721 ggctcacaaa gtcaggaaga actgctgcgg cagatgatag aaaggcgaaa tcgagaactt
    15781 aaccgataaa ataaaggaac aggtcttatg aacagaaaaa attatttgat tgtgattgcg
    15841 gcttttatgt gtctgctgac tctgccggca cctgtcgggg cccaagaagc cggcccagcc
    15901 gatcccaacg gcattgttga cctcgacgga cggtttcttt tgaactttca ggatgcgctg
    15961 gttgaagata ttctggatta tctatccgaa agcgtagggc ttgtgatcgt cgcggaaaca
    16021 tttcccacca agcgcatcaa cctcatcagc aaacagccgc tcagtattga tgaagttatt
    16081 tccctgatga atactattat gaaagaacag ggctacgcgg ctgtacgaat ggaccgcaca
    16141 ctgaaaatgg ttcctattga aaacgccaaa cagatgaatc tgccggtcac cactggaagc
    16201 gatccggata aaatcgtcgc cggcgatgac attgtaaccc acattatccc tatccggtat
    16261 gccaatgctg cccggctgcg ggaagacatc tctgctctgc ttcccagcta tgcggtggta
    16321 acatccaatg aggccagcaa cagtctgatt attacggata ccaccgccaa tatcaagcga
    16381 atctgcaaga ttgtcaaggc tgtcgatacg cagatggccg ctgttatcga tgttcgggtg
    16441 tttcggctga tgtatgctga tgcacgcaat acagcccagc ttattaatga agtctttgag
    16501 cagcaaagca cccagcagac atcccggtcg gatactaatc cgtttgcccg gatgatggaa
    16561 ttcggccgcg ggggtcccgg cgggcgcgac cgcggctcac agcagtcgga ccagagcagc
    16621 ggcggcaatg ccaacacgcg ggtcattgct tcggcggaca ctcagaccaa tgctgtcgtg
    16681 gtcagcggac catcggatgt gctggaggtc attgccaagg tggttgccga actggatgca
    16741 aatccccgcg atgagcggca gctgtttgtt tataaggtca aaaatgccaa agctgccaat
    16801 ctcaaggata agctcaacag cttgtttcag gagctccagc agctcaatca gatgggcaca
    16861 tcgcgtacaa ccaccggccg cggcggcacc gcccgcaatg tacagaccgg tttggggggg
    16921 atgttcgggg gggccaccgg cacaactgct gccagctccg acatttcgga tgaggtgtat
    16981 attgaagcgg atgaagatac caactccctg ctgattatga cgtcttcgag aaattatgaa
    17041 aagataaaga agatcattga agaccttgat tatccggtgc cgcaggtgct gattaaggtg
    17101 ctgctggctg aagtaacaac cagccatgat atcgatttgg gggtggagtt ttcgcacctg
    17161 aatctgcagc ccgacggcga tagtcaggaa gttaaaacca tcttccccac caatcttgcc
    17221 agcggcgaca acaccagcgg tctgacgacc aaaattattc aatcggattt gacggctacg
    17281 ctgcgggctc tggaaaaaat cggccggctt aatgtcttga gccgtccgta tatcctgacc
    17341 agcaacaacc agaccgctac catcaccgtc ggtcaggaag tccccttcat ccgtgatacg
    17401 cgaatgaccg aaacgggaca aaccatcaat accattgagt acgaggatat cggcattatt
    17461 ctggaggtta cgccgaccat caatgaagac gggctggtga ttatggatgt caagccggaa
    17521 atctcaacca caaccgctga aacagttccg atttccgaga cagtcaatgc cgcggtattt
    17581 gccaaacgct cttcctccag ccgggtggcc gttcagaacg gacagaccat cgtcatcggc
    17641 ggtctgatgc aggataccga aaccaaatcg gtcagcaagg taccgctggt tggcgatctc
    17701 ccgctggttg gcgaactgtt caaacgcaca accgtggaaa aggaaaaaac cgagctgctg
    17761 atatttttga cgccgcaggt ggccagcaat atcaacgatc tgaagaaaat ttccgatcac
    17821 gagcgcagca acagcgatgt gctcaaaacc acagacaacc ccgacctgca gaaacatctg
    17881 gacaagctgg aatcggttta caataaatag gaactgcctt gtcactttac ccgaaggggc
    17941 cgcagcagca agcggcccct tctttttttc cgcgctgcca aaggacagcc aactttgtct
    18001 tgcctgccga ggccaatctg ataaaatctt ctcaagaaca aacgcagccg aaattgtatg
    18061 ctgaaaatct atgccgtcgt atgctcggat aatccgtttg caaaatatgc tgtccaatct
    18121 gccgctggct tccctgctgg ccgccaatac cctgcctctt ttcggagtat tgtttctgga
    18181 ctgggacgcc tttgcgatgg tcctgctgta ctgggcggaa aatgtaatcc tcggttttta
    18241 caatattgta aaaatgattc tggtgaaatc cccccatccg gccgggcatc tgggcaagct
    18301 tttcatgatt cccttttttc tgattcacta cggcggattt acagctggac atgggctttt
    18361 tgtgatggtt ctttttgggc ggactatctc cggcggcatc ttgccgcagc agtcatggcc
    18421 ctgctttttg gtttttattc agctgttatt ccatgtcatc ggggaggtta ttggaatgat
    18481 ccctctgaat atggtatatg ctattgccgg tttattcatc agtcatggca tctcattcgg
    18541 ctataatttc ctttacagag gtgaatatat taaaacaaac ttgcgaaccc tgatgtcgga
    18601 gccctatggg cggattgtta tactgcatat cgccattttg ttcggaggtt ttctatcgaa
    18661 aacgttaggc tcacctgtcg gcatcttggt gctattggtt gggctcaaga cattcattga
    18721 tgtcaaactg cacctgcgtc agcactgttc aacactgcaa gcaaccgata acagatgacg
    18781 ataaacaaac aaaaacccga actgctcgcc ccggccggaa cgctcgaaaa actcaaattc
    18841 gcctgtgcct atggggccga tgcagtctat tttgggatgg agttcggttc gctgcgcagt
    18901 tttgcaggca atttcactct caaagatgcc aaagacggtc tggattatct gcacgctcgc
    18961 ggcaaaaaag ggtatatcac gctgaatatc taccccttca gcgaggaata cgacaagctg
    19021 atccgcttgg caaaacagct tgacagcata ggggctgatg catttatcat ttcggatgtg
    19081 ggggttcttg cggaattaaa aaaactccat ctccatgccg ctgtgcacat cagcacacag
    19141 gccaacacca ccagctggca agccgctctt gcctacaagg aactcggggc aaaacggatt
    19201 aatttagccc gggaattatc gctggaacaa atacaggaaa ttcaactgca tcttgccggt
    19261 caggttgaca cagaagtctt cattcatggt gcggtttgct tcagctattc cgggcggtgt
    19321 gctatcagtg attaccttgc tggtgctcgg gccaatcgag gcgagtgcaa acacccctgc
    19381 cgctggaagt attatctggt cgaggaaaaa cggccggggc agtttctgcc tgtctttgag
    19441 gacaaccgcg gcttatatct gtttaacagc aaagaacttg cgctatggga gtatgtgcct
    19501 gcattagcgg cggcaggcat cggatccttt aaaatcgaag ggcggatgaa gtcaattcat
    19561 tatttgtctt cagtcgtgtc attctaccgg caggtcatcg acggtaaaac tttcacacag
    19621 gaagaagggc tccagctgct caatcggatc cccaatcgcg gctattcagc aggatttatc
    19681 aggggagcca ttatgccgga agattattct gtcgataaaa gcatctccga aggcacatct
    19741 gtttttgtcg gcaatgtcat tcattctgct gccgacggct gcacaattga agttcgcaat
    19801 actatccgct caggcgatac ccttgaagtt cttacgccag acggccagtt atccgaaatt
    19861 accctctccg ttcccttgaa aacggttgca ggtgaatacc gcgatatcgc caccaacggc
    19921 caattcatcc agctgcctgt ttttcttcct gaattttcca tccttcggcg gctgggagat
    19981 ataaaactgc agaaatcttc ttgaaaaaag ctctgtgttt cgattacaac ttctgccgat
    20041 ataaagtaca aggttcttaa agaatgaata ttaaaggcaa gaaatatcat tttatcggca
    20101 ttggcggcat cgggaccagc ggtttggcca aggttctaat gaagaacgga gctatagtat
    20161 cgggttcgga tatgtccgac agccccgtca tcagccagct ccgcacccgc ggcgctgccg
    20221 tcacactggg ccacagccct gacaatatcc ctccagacgc cgacgaagtc gttatcagcg
    20281 ctgctattac aggggccaat cccgaattgg caggcgcttc cgagcgcgga cttcctattt
    20341 ataagtatgc tgaaatgctg ggcttattgt ttgaccggta tacgggtatt gccatagccg
    20401 gcacacacgg caaaagcact accagcggct ggctggcatg gattttggat aggggaggcc
    20461 tgaaaccaaa ctttattgtc ggcgcggaca tcccccaatt aggcggctcc agcggtgttg
    20521 gcaatggcca tttttttgtc gccgaggcct gcgaatacga ccgcagtttt ctgaatctgc
    20581 acccaacaat tgccgttgtg ctaaatattg agcaggatca tctggattat tatcgcgatg
    20641 aagacgaaat tgttgaggcc tttggcgatt ttgtagaaca agtcaagccg gatgggtaca
    20701 ttatagcaaa tggtcaggat aaaaatgtcg cccgcattct acggcggttg aaaacaggcc
    20761 ctaaaatcat aaccttcggt cttgaaagct tttgtgacta ttcggctcgg aatctgcatt
    20821 tgtccggcgg aagttatgaa ttcgatgtgt accataatga aaccctgtta ggacatatcc
    20881 gtatcgggct tcccggacgg cataatgtac tcaatgccct cgctgttatt gccgcagcag
    20941 aagcggccgg tttaaattgg tccatcctgc agcgggaact gggggatttt gccggcattg
    21001 accggcggct gatgcttaag gccaaaatcg ggggcatcac cattttggat gattacgctc
    21061 atcatcctac agaaattcgt gcttcgctgg cggctatccg agaaaaatat gctccgaagc
    21121 ggctgtggtg cctttttcag ccccatcaat acagccgaac ccgttttttg cttgatgatt
    21181 ttgcagaaag ctttaagctt gccgatataa ccattgtgcc ggaaatctat ttcgtgcggg
    21241 acactgccga aagcagaagc aaagtcagcg cagaaacgct ggtcgaacga attacccaac
    21301 atcagtgtca agctctgttt attaaggatt ttgccggtat agtggagtat ttgaaacaaa
    21361 atgtaactga cggcgacttg gttgttacaa tgggcgccgg aaccatttgg aaggtagccg
    21421 atgaatatat tcagtggctt ggaaagcatt gttagaaaaa actgtcccct gcgggatttt
    21481 acctggtatg ggctgggggg caatgcagag tatcttattc agcccaagac agccgaccag
    21541 ctaagagaga tcctgaatcg gtgccgggaa aacgatatgc cggtacgggt tctgggattt
    21601 ggatcgaacc tgctggtcag cgatgaaggc gtgcgcggtg cggtgctcaa actggatgcc
    21661 gatgcgttct gccgctatga gttcagcggg acaaccctga cggctggcgc tggagtcaac
    21721 ctcaataagc tggtgcttga atcggtccgc aaaggcttag gcggattaga ggcattgact
    21781 ggcatcccgg gttcggtagg cggtgcggtc cgtatgaacg cggggggcaa tttcggcgac
    21841 atcggctctt gtgttaaaag cgtaactgtt atggataagt acggcaatca gtttgagaaa
    21901 gaaaagcccg aattggtgtt cgactaccgc tggaccaaca ttactgcccc ggtcattctg
    21961 tctgcaacga tcgaattgac cgagtcagac ccggatgcaa tgcttaaaac agtcaaagag
    22021 gtctggattt acaagaaaaa ctcccaaccc ctgaatacac ggaatgccgg atgcattttc
    22081 aaaaatcccc gaggtatgtc tgccggcgcc ctgattgacc gtgccggatt gaaggggacg
    22141 cagttgggcg gagccgttgt gagtgaaaag catgccaatt tcatcgtgac tgaaagcggc
    22201 tgcaccagct ctgatgtcaa gcagctcatt gagctgatcc ggaccaaagt caaagaagtc
    22261 ttcgacgtgg atctcgaact ggaaattgaa atctggtaat aaataatccg aaaccctaag
    22321 tacaaaaatc caagtaattc atttgcatta agacacagac atgcagggta ctgtgtttgt
    22381 tttggattga gaacttagaa tttcggattt tttcataaca acagccgata taaattaaat
    22441 gcagaaagaa ctgacacaac tgaaaattgc ggttttagcc ggcggcatcg gctcggaaag
    22501 gtctatcagc ttattgagcg gccagacagt atgcagcgcc ctcaaagaag caggatttac
    22561 ggttgttttg agcgatatta ctcccgacaa cacagctatt ttaggggacg actctattga
    22621 tgtcttcttc ccgattctgc atggtcagtt tggggaagat gggcatcttc agcagctttt
    22681 tgagcagcgc aatctgtgct ttaccggttc gggctcacag gccagtcggc ttgcttttga
    22741 caaactgctc agcaaacagg ttttttttca ggcagggctt ccgatagccc ggcatctggt
    22801 tgttcattcc tccgatacgg aggcggattt gaagcagctg ctgccccggc tggccgacaa
    22861 attcgttatc aagcccgtcc ggcaaggcag cagtgtcggg gttaccatta ccgacgatct
    22921 caatatcgcc gccgccaaag caatggaatg cctgcatcag tacggcgact gcatggttga
    22981 ggagtttatt gccggccgag aaattaccgc cggcattctc aatggaactg cccttccgct
    23041 tatcgagatc cgcagcaaag ccgcttttta cgattttaag gccaagtatg aaagcgatga
    23101 aacagaatat ttatttgata cgattaacga ccagagtctt gtgaaaaaga ttcaggacac
    23161 agcagctgcc tgctttaagg aacttggctg ccggcatttg agccgggtgg attttatcgt
    23221 taccaatgac ggccggccgg tcatactgga aatcaatacg ctgccgggat ttaccagcca
    23281 ttcactgctt ccgatggctg cccggaaagc ggggatttca acgccgcagc tgtgccggca
    23341 gattgtcgag gcggcatgga aagactataa caaccgttcg catcggtgtt cctgtcgccg
    23401 aagcggcagc tgagcttaaa accgtctttc ggcggataga gtctgccggt tttactattg
    23461 tgtactattt cgctgatgac acgaaaaaaa gcaaaatcgg atacaggttc aggcggcttc
    23521 ttttttcaaa agaaaaaacg ccgcaaaagc ggcaccgcta tttcgtgggt aacggcgctg
    23581 aaaattaccc tgtcgattat gttcctgaca cttctggccg ccggaggtgc tgtcggtctg
    23641 atatatcttg agcgttatgt taaagaggct gctgctgcac aaacaccaac gggttcgctg
    23701 aagctggtca actcgccggc gtggctcaat caggactgga ttgacgcctt ggtaaaggcc
    23761 gccggcggca aacggtttga gctggatgac aaatctgccc gtcaggtggc aagacggctg
    23821 gaacagttgt cgtggctgac cgatgtgaga gtgcaaacca caccggaatt tctggaagta
    23881 acagcaattt atcgaagacc tatcgcgata gtttcggtca gtcgaacccg caaattttat
    23941 ctggatgcgg atatggttgt actggattat atccccgttt cggctattcc cgttatcgaa
    24001 atcacgggac tggccaaaac caacgtatcc gaaccggggc agatctggct ggccgatgac
    24061 gccaaagttg ccgttgaact tcttaattgg ctgtatctga tggatgtgca ctttcagcag
    24121 gaacaaaaac tgcctaaacc gctgatagat gaaatcataa gcgttgacgt atccaatttc
    24181 gccgctcgga aaagccggtc tgcccccaat atcgttttaa acgtcaaaga cggcactcgg
    24241 attctttggg gagctgcctg ggggcagtca gatgtttatt tagaagccag cgaaaagatc
    24301 aaagtggccc gcttgtatga atactttatc gaccacggct ataccttgca gggatccgca
    24361 aaaaatattg aactccgctg gctggaagac agcattccgc ggccccagta agaatgattc
    24421 tttctgccta ctggcaaaat taccacagcc atcggcacag atgcgctgac aggagtccca
    24481 aaaatgtccc gacgatattt cccagcacag ccatcagcaa gcccacaggt gcaagctggg
    24541 gatgataaat gccggccgtt acaggcgcag aagcagttcc tccgatgcag gcctgactgg
    24601 cggcagccag cagagccagc gggactttca tcaaccgtgc tgcggccagc agacaggcag
    24661 catgaatcac tatccagatg actccgactg caagaaaaac cggcagattt cctgcatatg
    24721 acaaaccagt tttcgctccg agggaagcca gcaccagata cagcagcagg aagcccgcac
    24781 ggcttgagcc gcaggtctgg agtctgcggg cgggtgtgaa agacagcccg attcccatca
    24841 gtgtagccaa cataatcgac catacagcag gattaataat gccctgcacg gaaggaagca
    24901 atcgaccagc ccagacagat atgccggcgg cagcagatgc gatgatggcg acagcagcca
    24961 taccggcaat gcgattgccg ctatccgcgg gagaagattt ctgtctggca cgagcggcta
    25021 aatcgttcaa taaaccgatg cgggagccgt tccagcagtc aaagcggctt tgatgagcag
    25081 ccagcgacac cagcagcgcc atccatccat aggcaatcag cgtatcggtc agaataatta
    25141 gtccaaacag ctcatcgggg gttccaacag cctctttgac agcaatcata ttaaatgagc
    25201 cgcctatcca agagccgcat aaaggaccga tggctttcca tgcgtcatcg ggtagccaag
    25261 gacgcaacag cagcattgct gccggtcccc ccagcagaat acctattgcc gcaattgcca
    25321 tgacgcccag cgccaccggc ccgagacgga caataccgct gacatcagac gacagcagca
    25381 gcagcgttaa tgcggccggc agtacccatg ccgttatcca gctgtaaact tcactctgct
    25441 tgggcagaat accagcggta tgggctaaca tgggaatggt ataaatccag aacattgacg
    25501 gcaggatgct gaacagtttt ttgcccacag gccgggaatc aataaacact atcagcccct
    25561 cgattgtcag caggacaatc agagtccaaa caggatgagc ggttatattc attgcgttaa
    25621 aggcaaagca agctgaagac aatagcggtc ggtcattccc gcaatatagt cgcagacaac
    25681 ccgatacagt ccctgtgaag caatcatttg ccggtaaaag tgcggcattt tgtctgggca
    25741 caaacacaaa tcctcaaaca gcgccgccag ccaatcctgc gccttttcgg ctgtctggcg
    25801 gatggtcgga tccagataca tattctgcat caggaatgtt tcaagctggc aaagggccgt
    25861 atggatgctg tcggacagac caatcagcgg atggggatgc tcatagacct gttcgagccg
    25921 aacaattgat gcctcattta ttttttttcg gctggtttct ataacatcgc ttgccagaat
    25981 atccataatg gttttgacca gacgggtgcg ccggatcaca tagtcgtgaa tacggtcaag
    26041 tgcaatcttc ttttcggcca gcttatacag cgctaaaccg gtcagttcag acaggtcaag
    26101 cagccgagac cgaagaccgt cttcaagatc atggcaatta taggcaatcc ggtcggcaat
    26161 gtcggcaatc tgaccttcca aagacggtcg gacaggccag ccggagtgcc tgaaaggacg
    26221 gtcataaggg ctggtgtgtt tgaccaagcc cagacgtgtt tcgaacatca ggttcagacc
    26281 gcggaaatca ggataaggac actcgataaa atcaacaatc cttaaggcct gactgttgtg
    26341 ttcaaagccg ccatagtcag ccatcaaatc attgagaata tgctcgccgg tatggccgaa
    26401 aggcggatgc cccaagtcat gggcaagaca aattgcctct gtgagggcct cgttgaggtt
    26461 gagagcacgg gcaattgtgc ggccgatctg ggcaacctcg atactatggg tcaaacgggt
    26521 acggtaatgg tcgtccagac cggttgtaaa gacctgtgtt ttgccttcca gccggcggaa
    26581 ggcagcactg tggatgatgc ggtcgcggtc gcgttcaaaa gccccccgaa acggatgggg
    26641 cggttcgggg atttgccggc cgcggctgat agattccgta acagcataac tggcaagcaa
    26701 ttcggtcatc gtcggcttgg cagatgttcg gttcggctga tttcaacaag ttggtcgtag
    26761 agttcataaa gcccctgact gatgacatcc gtatcactgc agacatgcat aaagttggtg
    26821 tcgccgtccc agcggggcac aacgtgaata tgcaaatggc ccggcaatcc cgctccggca
    26881 cagcgtccga aattcattcc gacattaaaa ccgtgcgggc gtattgccag cgacagagcc
    26941 ttctggcaat cccgaaccaa ggtcagcaaa gtcagcaatt cctcatcgga tgcatcatcc
    27001 agcgtggcaa tatggcgatt gggagctatc aataggtgtc cattgttgta tggaaaccgg
    27061 ttgaagacaa caaacgcctg ttccgtgcgc cataacaggc aattatcacg gtctttctcg
    27121 acagcagcca gataatcaca cagaaaacag ccctccttgt gggaaaggcc ttgtatatat
    27181 ttgatccgcc atggcgccca gagatttttt cgttccatgg attcggctcc ttgcgccgtc
    27241 agggattcgg gatatactcc agtgatatgg tctggctgac cgtcaggacc ggcagcgaat
    27301 cacccaacgg cagcataaac tctgccatca cattcagggt gtactgctgc tgctcgcttt
    27361 caatcagccc ccggtcgata tcaaaaatct gtgtacctgt tccttcaagg gatttgaact
    27421 tgtaattgcg aagaaaacca aaaagccccc gcatctggaa actggtttca tcgtacggac
    27481 ggacaaaatc cttcatcggc tcgtcgctga gggcataacg gcattggata acagccttac
    27541 gcggctggtc cggttcggca gtaatatgat ccagcgtata ggttgtgatg cgggccggtg
    27601 gctgatagag cggagccggc caaggaacca gctgtttggt ctgccatgtt tgaccgactg
    27661 tcaaacgaac cggatccggc acagcggcgg ctgaatccca caaaaaccac tgcattgcaa
    27721 taaagtcata aagcatgtcg ggatttttaa tattctgggt ggcgctttta tcaaatgact
    27781 ccttgcccag ctctttggtt atccgctcaa ggtcgctgta atctgcgatt tcccccgtcg
    27841 gagacagctt caggataaac cctttgcccg gcagggtctc catagcatcc tgcgactctt
    27901 ttcggccgga aaacgatgaa cgggttactt tggcggatac gcattcggct ttaagggttg
    27961 tcaggccgaa ggggtcaacc tccatcggcg tgtagctcat caccaactcc agtttttcac
    28021 tcattgtctg agggcgagat ttcttggatg ggtcgctggt ggtcaagtcg atttctgtga
    28081 cccgctcgga gaccattttg taggtaatcg gcactccttt ttcaaacgaa accagaagcc
    28141 atgtatcctg cggcagctgc cccgtatggc ggcagccggc agtcatcagc acaagcggca
    28201 gaatcagcca aagccagcaa ttcttcacag cgattccttt cctgcaaaac cggcacagcg
    28261 gttagtctaa tttctccaag cgaatgcgat tggtaaactg catggtcagg gtatcggggc
    28321 ccttggcagg atcgccgttt tccggcatct cctgagcgac ataacggctc accaatgtct
    28381 cttcgctcag cagaactgtt cccgtactgg tatccagctt catggtgccg gtatagatgt
    28441 cttcgttatc aaacattttg gcaaacatgc ccatagaacc cgaagcagcc gtcccctctg
    28501 cagccggttc agccgtttca gaggccacca tatctatggt agcgatgtgg ccatccaaac
    28561 gggtaaaggt ataaaccttt ttataggtct ttggagccag caatcccggc ggtgaaggca
    28621 caacttgcgt ccacgagtct ttgagggaaa tgtcagccgc tttctctttc ggcaatgcgg
    28681 caatctggtg acgctcggca actgccttag catccagcaa acctgttaca agctgttttt
    28741 cgtatcccga ggaaacagag gccaaagcgg cttgtgcatc cagcagttca gctttgccat
    28801 tcggtgaaag cttaatggtg tagctttgcc ccagcagttt ggccagcggg ctggatttat
    28861 ctttctcgtc agcgctgttg aaggcaaatt tcggctcatt cttgttgatt acattaaccg
    28921 ccaagtcatt aatcgttacc ttgactgcgg caatgccgtc ggcatctaca ctttcgactg
    28981 tctgggtgaa gcccatctca atccatgtct tggtctgctc ttcacgcagc ttgccgaggt
    29041 tgggctggtc aaaccggaag tctttaataa gctctgtggt tgctttatag cgagcggttg
    29101 aatcgggagt caacttcagc cccagctgcg ccgagggtcc gcacccgctc aacagcactg
    29161 ccgcacccgc tgccaccacc gccagaatca caccttgatt gcgtgacata accgctctcc
    29221 ttaatgcata aaaggtttac cgttctatcc aaccatttcg gcgattgatt ataactgtta
    29281 acaactcatc tgacaacagg aaataagaca ggaatctgaa aattgtggag atttttttgt
    29341 ggcaatggag aaaaaaatcc agtcgctatg gttttcatag ccctttttta acttgtacgg
    29401 tctgaaaaag ccccggctgt tcagtttgag tcatttcagg ggtattttaa gaattgtcag
    29461 cgaatgcggc gaaagcttga cacttccatt gtctaacagc agctgcttct tttcaggact
    29521 aacccgatgg ggatgctcga tgtcattata ggcctcgggc atatcacctg ccaacactac
    29581 tgcctctaac cagcctgacg gcaggggggc tcctctaaaa tcgattgtgc agattacctc
    29641 ttgtaagggg tgcctgttga ccagagcgac tgtgcatact gactgtgcct cattgacggt
    29701 tccaatagca tcaagcacgg gaatcgattg gccgccgccg gcaagcggtc cagcccggac
    29761 ttccgttttg gcaacccgac tgcccagcag gttggcatac attgccattg tatggaaggt
    29821 tgttcgctta actatcccgc cgggatacac atacagcggt ccccgtgtat tgaccagcgg
    29881 ggcaatgttg gccattccca catcctccga atgccgcagg caggcattga aaaacgaggc
    29941 cgaaaacaga gcatcggcca tcgtatattg cgacgggatc aaatttttat cccttgcccg
    30001 gaccaattca ataatatggg gatcattata attgctgact tccctgcgcg gaaatcccgg
    30061 atggtgccag ccgcgcagat tccattcatc gaaagcgatt tttatacgcc cgcggtatcc
    30121 gtacttttca aggagctgga ttgtctggac aatctgattt tccggaccat tggagtacat
    30181 cacacaggtc agataatccg gctgctggtt cttttcattg catatcagcc agtatccgtg
    30241 tatagaaata taatccaagt attggcctgc cgcttcaagc agccggccca cccattcgtc
    30301 gcttgctacc gccgctgcag ccagtttgat gcccgcatcg acggccttca ttctttgggc
    30361 cgcttccctg accatcggcg cccactggtc ggctgtttta tggccgattt cccacggtcc
    30421 gtaattttca tttccgatac tccatagttt gacattgaaa ggggccaaat gaccgttggc
    30481 aatacgcaga tatgcagcgc ctgtttcggg ggtattgcag tattcaaccc actcgaccat
    30541 ttcgtcaatt gtcccgtttc cggcattgtt gcagatatag ggctgccagc cggccagccg
    30601 gcaaagctcc acaaactcat cggtgccgaa ggcattggac tcccttacgc cccaagccat
    30661 atcatcaaca ggaatccgat tgggaccaat gccggctttc cagtgatagc cgctgacaaa
    30721 acagcctccc ggccatcgga cgacaggcac cttcagctcc ttcaacgctt cgataacatc
    30781 cagacggaat cctttttcat ttgaaagcgg cgattgcggc tcatagactc ctccatatat
    30841 ctggcgatta aaatgctcca gaaaacctcc gaaaatcatc gggctgtagg attttgtatc
    30901 attgaggaaa actgttactt tcgatacttc tttcgcttgg gaagaaccga aatcagtcac
    30961 agaatctgct gattggccca aaagagcagg aaatactgtg aaaaaaacag cggcggctaa
    31021 aatggactgc ttatacattt aagtgtgcct ttcaaaatgg gagacttgaa tgtcgcattg
    31081 ttaatctcga tatgaaacgg cattttaaca taaaacaaaa ggctgtcaat tcaaacaggc
    31141 tgagctcctt tttgtaattt tgggattggc taaaactgcc tagactgagt ataatttatg
    31201 agtttttggg tgaccgtaaa aatcggattt ttagaatcga agataaaatt gatgacaggt
    31261 tcttaataag ggagacggct atgaacagtg acagaattaa aaagggattc cagcgtgcac
    31321 cgcacagggc gcttttgagg gcctgcgggt tgaagacaga agatatgaac aaacccttta
    31381 ttgcggtggc caacagctac tgcgatatcg tgccgggaca tgtgcatctt cacgaagcag
    31441 ccaaggcggt caagcaggcc attcgccaag ctggggggat accgtttgag ttcaatacca
    31501 tcgccgtctg cgacgggata gccatgggac ataccgggat gaaatattca ctggcatcgc
    31561 gagagattat tgccgatgct gtggagacga tgattcgcgc ccactgcttt gacgggatgg
    31621 tctgcattcc caactgcgat aaagttgtgc cggggatgct gatggcgacg gtgcggctga
    31681 acatcccgac actgtttgtc tccggcgggc cgatggctgc cggcaaaccc aaaaacggca
    31741 aagctacaga cctgattacc atttttgaag gagttgctca atacaacgcc ggaaaaatta
    31801 cggaagcaga tctgacagag ctggaatgca tcgggtgtcc gacacaggga agctgctcgg
    31861 gaatgtttac agccaacagc atgaactgct tgtgcgaagc agtcggaatc gccctgccgg
    31921 gcaacggcac cattctggct gcagcgctgc ggcggcagga attatacaag caggccggca
    31981 ggcggattgt agagatggtt aaagaagacc ttcgtccatc gtcgattatt acggaacagt
    32041 cgctggacaa tgcttttgta ctggatatgg cgatgggcgg ctcaaccaat acggtgctcc
    32101 atactctggc tgttgccaat gaagcgaaga taccgtatga ccttgaccgc atcaacgcca
    32161 tcagtgccaa gtgtcccaac atctgcaagg tgtcgccgtc aagccactgg catatcgaag
    32221 atgtagatgc cgccggcggc atcagtgcga ttttgaagga aatcagcaaa acggggctgc
    32281 tgcataccga ttgcatgacc gtctccggcg ttacgcttgg acagcggatt aaagatgcgg
    32341 acattaaaaa tcctgactgc attcggccgc tggaaaaagc atactccaat acaggcgggc
    32401 ttgtaattct gcggggcaat ctggcaccca acggctgcgt ggtcaaagcc gccggcgtcg
    32461 caccgtcgat gctcaagcac agcggtccgg cagtcatttt tgaaagtcag gaagaggcct
    32521 gtgagggtat tttggccggc aaagtcaagc cgggggatgt agtgattatc cgctatgagg
    32581 gtcccaaggg cggaccgggg atgcaggaga tgctgtcgcc gaccagctac attatgggtg
    32641 cgggtcttgg cgagtcggtg gcgctgatta ccgacggacg tttttccggc ggcacacgcg
    32701 gggcctgcat cggacatgtc agtcccgaag cagcggtcgg cggtccgatt gccttagtca
    32761 aagaaggcga cattgttgag attgacattc ccgccaacaa agtggatgtt aagcttagcg
    32821 atgccgagct atcccagcga aggaagtcct ggaagccgcg cccgccgcga attacggaag
    32881 gctgtttagc caaatatgca gctatggcta ccagtgcgga tacgggcgct gtgttgaaat
    32941 gggactaact gcaggccaac aagtaatatt gcaaatatga aaagaggcgg ctggaatgca
    33001 gccgcctctt ctttttttta aagccgctga ttggcagcgg caaattctct acttattaag
    33061 cggccttttt acagccggcg ccggttttag ggcagccgtt ttggcctttg ccgcacttgc
    33121 cgtcagaaca gctgcaagta ccacacttgc aggtttcgca tttgcacgaa tcgccgcatg
    33181 aacccgcctt gcaggtgcaa tccgtgcatt gacagtcggc acagcattta ccctcggcgg
    33241 gcgcctgcgt gcagcagctt gccatagccg atgccgctgt cgattcacta tgcgagacag
    33301 caggggtacc ggaaaaagca aacaaaatcc cggccgcaat tgttaacacc acgattgaaa
    33361 tgattatttt attcatagta tctacctttc agtttgattg gaaaaaattt gatatttttt
    33421 aacaacaaaa acagatcagt gatggataat atcaacaatt cagctggcaa atcctctctt
    33481 gtattgatgc attgcaatat ggcagtacag agataacaga agggaattct atttcgaggg
    33541 tccccaacgc aggaagcatt tgaagatcca gtgctgttgg gtgtatagct ggaggttcaa
    33601 aatttaaggg ggaaaccagc caatcacagc atgtctgagt acaggacata gcacagcagc
    33661 aattgacaga atatgaagaa ctttccgaag attgccggcc agaacagcag gaatctgatt
    33721 gctcagaaac cttgcttttt aagctgaaat cagaaatgct gcagtggcat aaatgctgtt
    33781 ttgcaacagg aagcaccgca actgctaaca aaccggccca aaaacctata tatcgctgga
    33841 tattcatctt tattctctaa attttatgca tattttacaa tttatacgaa ttaggacacc
    33901 aaaaggtttt ttttattata aattcaattc gatattggtt tgtcgctatg ctttgtctgc
    33961 aatccattat tgccctgtat ttttcgaagt tcttgaccgt ctaaaaaacc acaaagaaca
    34021 ggccccaatc cccgcccatg ccagcgatat tactcagaat gactcaaatt gagcgggcgg
    34081 cttatttata tgctggagat aataacgccc tccatccgct cccaacaata tttcagatta
    34141 cagaaatgtc aagatttacc gatagttaag tttcccagaa aaatgccaac tgtctgttat
    34201 gggatcagtt catggacatt tgacagtaaa atagataggg tcgtccatgg aatcacctcc
    34261 cctactcgat taagcgtatt tgcccatccg ttggcaactg agcacatagc tgaaaataaa
    34321 aagctccttg ccggccggtg atacaggaaa aaaccgattt tgatattcag tgttttgata
    34381 tcgtgggaga ttaagggagt ggcctacaaa gtaaaaaagt tattttttgt aaaaaagaaa
    34441 agtggtacac tgtctggtac gtgatatatc tttttaaggt tttgcaagaa tcaaaaaata
    34501 tggttaggtc gtgttcatct ggagttatac gagaccgtat attgcggatt gcggtgtatg
    34561 tgatcctggc gacactgaca ggaattgtcg gcggatgcat caccggcaaa aagacatcaa
    34621 cagccgggcg ggcggactat ggttttattt ttctgggcga cctccatttt gaccggccgg
    34681 ggcatcatga tatggactgg gtctgttcca attatcccgg cgatgttgtg cagattcagg
    34741 attattgccg gattaccgag gagcatacac cggggctgtt tggggcaatc agcgggacca
    34801 taaaagagcg gccgcatcca gtattcgctg ttgtgcaggc gggggatttt gtggagggct
    34861 tatgcggcag ttatgaactg cagaggatac aattcgaaga ggcgaaaaca tttgcggagg
    34921 gatatttcaa gacggttccg tttttaatca gcaagggtaa tcatgacatc accggtccgg
    34981 cggcacaaga agcgtaccgg gatgtcatac tgccgtggat aggcggccag cttggccgag
    35041 ccgtcacaca ggcgtcatac gcggtccgac gaagcaatga cctgtttatt ttctttgatg
    35101 ccattcgtcc ggaccttgac tggcttgaag aaacgctgcg gaattcgaat gcccggcata
    35161 cattctttgt gacgcacaag ccggtggtgc cgtttgatgc aagggcgaac tggcatctgt
    35221 ttgccgaaga gggagccgca gcgcagcggg aacggctgct ggaattgttg gggcggcatc
    35281 gggcgattgt gctgtgcggg catcttcatg cttattcgct gctgagccgc aagacagccg
    35341 gcgggggatt tgtacagctt gccgcaaaca gcgttgtgcg tcaaaaggag ggagcaattg
    35401 aaaaatattt tgtgagcaaa gatgcctatg gggccgattt ggtgagcctg gaaccgtcat
    35461 tttcgccagt gaccttagag gtgcggaaac acaatctgga gactgaaaaa ccggagattg
    35521 gattttttga gtatgcgcgg accgccggct ggtgttttat ccagtgctgt ccggataagg
    35581 tggtggttga ggtctatccc ggctgcagtc agacgccatg gcgagtatac gacatttcct
    35641 tatttggcgg caattacaca atcgagaaag caaaaccaga tgctggccgc cgacaccagc
    35701 gagaggatac cggtatgccg gaaattacag tggaggtcga agcgggtatc gggagttttc
    35761 cggggcacaa atgagattga aaacagaaaa cgcagatttc acagaaggaa tttttttgac
    35821 gggattaatg ggattaaaca aataaaagaa aagagataca gtattcttta acgttcccat
    35881 ttggggatag ttaagggtcg tactttgttt cttgttatgg tctatcatgg atcgttttaa
    35941 gctgttgcaa aatccagaaa aggagtaatc tttgaatcgg gaagtaatca agagctcgca
    36001 tttgagatat tcttatcttt ttatttgttt taaatgtgag tttatcgcta ttgaaaaaaa
    36061 cgcgataaga atttgactgg agttgcagta cttttaaccg ttataattat cttgagttgt
    36121 ttctgtaaat cggtcctaaa tttctaagtt aggctgcaat tgaacaatac atggtattca
    36181 tcgggtttgc ggtttgagtg tgtcgggtgc gggggatgct gcgcagggcc ggcggagggg
    36241 tatatctggg tgagcaagcc ggagatagag tttctggcac ggcatcttgg ccggtctgtg
    36301 aaggatttta agaagatgta tctgaagcgg attggactaa aatacagtat ccgggaagac
    36361 ccgcttacga aggactgtgt ttttcttgag atggtcagca acggctgccg cagctgtgct
    36421 gtttatgcgg tccgtccgaa ccaatgccgc acatggccat tctggtcatt taacctgcac
    36481 agccccgagc agtgggagat ggcggcgcag cggtgtccgg ggatcaaccg gggacggctt
    36541 tatacggctg aggagattga agcgataaaa aatcagcaaa catggtggga taatactatc
    36601 aaaaaacagt aagatgacga acagtccttt gggagggggc ttttattgat tcgcatttta
    36661 tgcataaaca gttaatagaa gaggttgcag caatttaccg gcggctggac gagcaaacag
    36721 cccctttggc aggcggctgc acagcctgcg gggagtgctg cgattttgag cgtttcggac
    36781 atcggctgta tgtaacaacg cctgagcttc tgtattttgg atattttgcc cggcagccga
    36841 tacaggaaat gaatggcggt atttgccctt accgcatcag cggaaaatgc agcgtttatc
    36901 cgtatcgttt ttccggctgc cgcatttttc agtgcagaca agatagtcag cagcagcaga
    36961 acactatcag cgaacagaca gtccgccaat tgaagacact atgcacccac tatagcattc
    37021 cttatcatta tgtttacctg caggccggat tgaaaatgct gcggacaggg atgctgccgg
    37081 agcagctgta aaagaccggt actgttatat gccgtctgtg caaaccgtca atctgcccaa
    37141 tcatgcaggc cgacaatgtc aatccggcat tggggaatag catccaagaa ctgccggccg
    37201 taggatttgc ggacaatgcg gctgtccagc acaacaacca tgcccgtatc ctgccgggta
    37261 cgaatgagcc gtccaaagcc ctgcttgaac ttgattacag ccattggaag ctgataggca
    37321 aaaaaaggat tttcgcctcg ctgcttaagc atgtcgatgc gtccggcaat cagcgggtgg
    37381 ttcggcacag caaacggcag tttgacaata atgacgttgg acagactttc ccccggcaca
    37441 tccaccccct gccagaagct gtcggtaccg aacagcacgc tgcgggatcc ctgcttaaaa
    37501 cgtgccagca ttcgcgctcg gtcgagggtg ccgccctgta ccagcagctc cattttctgc
    37561 tcagtcatcc agtcccgcag ccgattggcc gtttcggtca gcattgagta gctggtaaac
    37621 agcacgaacg cacggccgcc gcttttgagc agatattttt ttatcgcctc acaggccggc
    37681 tccaaaaaag cctcttcatt gggatcgggt aaatcggcct caataaacag ccgcacctgc
    37741 cgctggtaat caaaaggtga accgacctgc agggctttgt aggtatcaag gccaatgcgg
    37801 tcggcaaaaa atgcaaagcc ctcttcatct cttccctcag gcgcacagct tagggtagca
    37861 ctggtcagca caaccgattt gtaggcatca aacagacagc gcttgatata cgggcctgca
    37921 tccagaggag cgctgcgcag gtggattttg gtgcgt
//
LOCUS       DAOTBO010000019        37014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_901_length_37014_cov_5.623285, whole genome shotgun sequence.
ACCESSION   DAOTBO010000019 DAOTBO010000000
VERSION     DAOTBO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 37014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37014
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_37014_cov_5.623285"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PLE88_04095"
     CDS             <1..1228
                     /locus_tag="PLE88_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75682.1"
                     /translation="HPMEGADGNTDGTPSPLTFRRYQRFAAGGAGLIWVEAIAVVPEG
                     RSNSHQLWIHKENKKAFSELIYLIRRTANDSIGSKHRPVMVAQLTHSGRYSRPKDCPE
                     PMILQSDPYRDALCPMPVPAANYASALPPDKTVLVDDEYLDNLQWKYIEAAKLAFDAG
                     FDAVDIKACHGYLISELLSSRCRQGKYGTTFENRSRFLLEVIDKIHQNLGADCPISCR
                     LGFYDAIPYPYGWGVSHDDFSKADLDEPKKLMGLLVQRGVRLFNFTIGNPYLNPHLGR
                     PFNRSATDGYISPEHPLKGVERMITLAGELQKCYPDAVFVGTGYSWLGPMMGYAGAAA
                     KALGKSKMIGVGRMAFAYPDFAKDLLNKGTLEENKVCIACSGCSQLLRRGLPTGCIIR
                     DSSFYGKEKKSKSPTS"
     gene            1191..2834
                     /locus_tag="PLE88_04100"
     CDS             1191..2834
                     /locus_tag="PLE88_04100"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOM75683.1"
                     /translation="MGKRKKANPQPADNSSPLPPAGIRRHWAASEAAAGAGLALFLFT
                     IYTATMLQGVDAGDSAELQFMSPLLGVCHPPGYAIEVCFGKLFSMLPFGPSAAWRINF
                     MMVISGTAGCLALYGTIRRITGSIIAGVVGALTLGLSSIYWSFSVAAEVYVFYAMFLL
                     WGLYCIVRFIDSKRSVWLYAAAAVLGICTADRISEILIMPGFLLLWASVRKEVKLSIL
                     QLLAAAIVFILPFLFTVSFHLIQNHPQKRTEALYGRDSALRFQILADHFEVYIPPLLQ
                     RIKGAVQYCLGLMYKKDAKFNTSVMAWDMNKYVWLLSGMGAAGERFPSGDRRNSEQGT
                     GTSIGILGLAAAAAGLIQHRKNWGWLLLGLWMFSANLIFILWHHRWDNLTFTIPGLAG
                     LAMLAGIGASGGQEAGRKTVIYRYICLIIPLFLLVTNYPLVNRNTAAEKKNLEQLQKI
                     SKASFAANSAIITTYWPAMTYRYLIQLEGGREDIRIIHEETKNWQKLVDYFLNKGLPV
                     YMEKNRMNPDILQQAQKQTETDVWQAGFVKLPQLEPHRN"
     gene            3000..4119
                     /gene="prfB"
                     /locus_tag="PLE88_04105"
     CDS             join(3000..3059,3061..4119)
                     /gene="prfB"
                     /locus_tag="PLE88_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_123785094.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOM75684.1"
                     /translation="MELAELRTSIGDLEARIEQVGTGFDVPAKIALRDKLEKQIAQPH
                     FWDNPETARQVVTQLSTVKSVIAPIQEAEKTLQDIHELFVLASEEGDEATLASIQADL
                     EKLAAQCDKIEIAGMLNGPDDSRPCFFGIHAGAGGTESCDWAAMLLRMYTRYFDDNKY
                     RYEQLDLTPGEEAGIRSITLRVEGPFAFGKLSCETGVHRLVRISPFDAAGRRHTSFAA
                     VDVMPEYEDDIEIDINEEDLRIDYYRAGGAGGQHVNKTSSAVRITHLPTNIVVQCQND
                     RSQHKNKAEAMKLLKSRLYMLEQQKRDAELAKLYSSKGDIAWGNQIRSYVLQPYQMIK
                     DHRTDYQSGNVQAVLDGDIEPFIESYLRYRAQKKHKPS"
     gene            4116..5600
                     /locus_tag="PLE88_04110"
     CDS             4116..5600
                     /locus_tag="PLE88_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOM75685.1"
                     /translation="MKTHPQKLYSRRHFLKTAAASAAAVYAGCCSTQQICRRQSAGKP
                     NIIYILADDLGYGDLSCYGQKRFSTPHIDRLADEGLLFTRHYAGSTVCAPSRCSLMTG
                     LHTGHCQIRGNLSVEPEGQWPLAAGTKTAASVLKEAGYSTGAFGKWGLGGPDSSGDPN
                     RQGFDEFFGFKCQTMAHKYYQRYLWRNNEKVYLPGNDPEKQTGQYCHDLIVDQAIEFI
                     KTHHKRPFFAYLALTIPHAELAVPADSLEKYKGLFPEDNPFIPNPNQDYGRQLHPRAA
                     YAAMIDRMDRDVGRILTLLKELHIDDNTIVFFSSDNGPHKEGGGDPDFFDSNGPLRGY
                     KRDLYEGGIRVPLLVRWPSVIKAGTRTDHISAFWDTLPTFAQLAGTQSFPDSDGISFL
                     PVLLGNPDAQLRHPYLYWEFHERGGKQAVLIGNQKAVRLNAMNNPDAPLELYDLAEDP
                     SEENNIAAAHPQLIEKMTFLIHQAHKPNKTFPFFASERNSSLQR"
     gene            5625..7151
                     /locus_tag="PLE88_04115"
     CDS             5625..7151
                     /locus_tag="PLE88_04115"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOM75686.1"
                     /translation="MTKQLIDIAVGVQKTDIFTCAADIAAVGVFEDNYSGHLIKELDK
                     RLGGQIKFIEKLGDFDGKAASLCLLYTNGKLAAKRLLLVGLGKRSKLTAAVLHKCAAA
                     AAGKAVEINAGSAVFCLHSDAAAKLAMEDIGQILTEGAMFGAFRYDEYITNPEKKRPA
                     RLKIILADSSPAVVKQLKQGAAAGIITGSAQNYARAFANRPANVITPAVLAAEAKRLS
                     QISSRLRCTIFNQRQLQTKKMEGILAVGQGSNNPPRLIVLEYTPAGASGRLSSVALVG
                     KAITFDSGGISLKPGEGMQDMKFDKSGGLAVLGAMRAIAALQPKVRVFGIIPAAENMP
                     SGRSYRPGDIITTYSGKTVEIHSTDAEGRMILCDALAYAANTLGCPVIVDIATLTGAC
                     VVALGQKRAGLLGNDDELMEQLKAASAKTGERIWPLPCDDEYLEAMKSKIADLKNTGG
                     KWGGACTAGAFLSQFVGKAKWAHIDMAGVGVWGGAEGETAGSIGFGVRLLTEFVRNYK
                     "
     gene            complement(7176..9473)
                     /locus_tag="PLE88_04120"
     CDS             complement(7176..9473)
                     /locus_tag="PLE88_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOM75687.1"
                     /translation="MNKTENKKHPYWNPDLPVELRVQDLLSRMTLEEKVAQMLCVWQS
                     KCGTFFDEKGHFNPQSSKLNLQNGLGQIGRLSDAAGGLTAYKTAQMANAIQEYMRKNT
                     RLGIPVVIHEECLHGHAALGATSFPQPIGLAATFDPELVMRLYQMTAEETRLGGAHQA
                     LTPVLDVAREPRWGRVEETFGEDPYLAAQMGLAAVRGFQGDGTFKDKKHLIATLKHFA
                     AHGQPESGTNCAPVNVSQCILREVFLYPFKKAVQEGPAISVMASYNEIDSVPSHANKW
                     LLQDVLRKEWGFKGYVVSDYYAIRELNERPGFFGQYLAENPKQAAALAVRAGVNIELP
                     DPDCYPHLIQLVKEGSIPESLLDNLVAPMLEWKFRLGLFEDPWVDPDAAERIVGCEEN
                     SRLAMEAARKTVTLLKNDGAVAPLNASAIKTLAVIGPNADRVMLGGYSGTPPRYTTVL
                     QGLRDFLGNSAEILYHEGCKITVGGSWQQDEVILSDPAEDRKSIAEAAKIARQADAVV
                     LVIGGNEQTSREAWDKNHMGDRADLRMVGLQDELVDAVAASGKPVIAVLFNGRPLDIR
                     HLAEKVPVIFECWYLGQETGPAVAEALFGQLNPGGKLPISFPRSVGHIPAYYNYKPSA
                     RRGYLFDSVSPLYAFGFGLSYTQFAFTNLRLQKQTIRPDESTTVCVDVTNTGSREGDE
                     VVQMYIRDCYSTVTRPVKELKGFQRISLAPGQTRTVHFDIEPEHLAFYNIDMDYIVEP
                     GAFEIMVGNSSRDCDLKKVILTVSG"
     gene            complement(9538..10944)
                     /locus_tag="PLE88_04125"
     CDS             complement(9538..10944)
                     /locus_tag="PLE88_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM75688.1"
                     /translation="MESNAQKLSIFEKIGYGLGDTASNLFFQIFIIFLLYFYTDVFGI
                     TAAQLGLMLLVTRFWDAVNDPIMGMLADRTVSRWGKFRPYLLFGAVPFGILGVLMFTT
                     PQFSPTGKLIYAYVTYIAMTMIYTAVNVPYAALMGVISPDSMERTVVSSFRFVLAFVG
                     MFIVQYALLRLVKLFGGGNEQLGWQLAAAALAGLAVVLFLITFFTTRERVQPIGQQRN
                     KLADDLKDLLTNKPWLLIGSATVFQLMFIVIRSSSIMYYFEYYLGNKELSFFGKTKVY
                     SFEDIVSAYLLTGTVFTILGAVLTKWIARAFGKSQTYSGGLAIAGIASALVFVLGPDD
                     IVLLFVLQFITSFAIGPVSVLQWAIYTDTADYSEWKTNRRATALIMAASLFALKMGVA
                     LGGTAQAWIMAAYGYVANQPQTPQALTGIRMVMSVYAGIFALLGAAVMLFYPLNNQRM
                     KTIESQLNERRQKNSAGN"
     gene            complement(11034..11795)
                     /locus_tag="PLE88_04130"
     CDS             complement(11034..11795)
                     /locus_tag="PLE88_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75689.1"
                     /translation="MRKKIGFTLIELLVVIAIIALLLSIMMPALKRAKEAAQRTVCIN
                     NLKSLGIANELYANQHNGAYVPILDRNRKDFQNHWITNMSFRANLDLDSFQSNSTMKY
                     KSPEKFLCPTDKIGKDPANAVQGVLISYGYNYTDWGWDMVDYAGHKMSNITSPATRIA
                     FIDSNDWWVQWSAADYRKGWDVLGQANIQAYKDVGLHGPTIYRHSEGANIGFYDGHAE
                     YNKKEEIFIIKDFEASPKQPGRWVVNPQKYLQNNL"
     gene            complement(11944..12840)
                     /locus_tag="PLE88_04135"
     CDS             complement(11944..12840)
                     /locus_tag="PLE88_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM75690.1"
                     /translation="MKKLNAAKIVLGVLCAAMSVSSPAAYRDVVMADNPLSFWEFEDA
                     SSANGDVCLDSATRAYKNGTYITNGTGVADIALVPGKVGQAAQLNGSGSGGAGNCIDI
                     WDGAAYPYSLSKDSFGYPLQTVSLEAWIKSSTTGGYARIMQHNGAYNTLDGYGIGSYG
                     STTGDITVIGAGTTWYTGTGNVFDGEWHHIVVTYNPLLDNEDGIDDMYEYVYIDGVGK
                     WGNVIEDGALGFSYDRLTLGSEGNRWYLYNGFVGLLDEVAIYDTVLSADRVAAHYAAG
                     IIPEPATLTLLGLGAIALLRKK"
     gene            complement(12951..14648)
                     /locus_tag="PLE88_04140"
     CDS             complement(12951..14648)
                     /locus_tag="PLE88_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75691.1"
                     /translation="MRSQYMLGLVLLLSGFARADNVVKTLYLDGVYMGQTTAEAALVY
                     AYDRLIIGAEGGVGYRYNEYNGKIDEFAVYAGILNSLRITAHYNALYSDPNAYVNQVL
                     SDNPLLYLRFEDPNSFNNSPAKNSGTANVNGTYIDSVSLSAGVAGKAAVFHGDAPDGA
                     GDCVDVSDASGAFSLEDITIELWMQITADPNTYPRLFQHNGGWDSPSSYGVMLVDANT
                     IGVIGGGTTNYFDTSGGVSIADGNWHHLVVTYDSTIEQITHKSYVEEVMDDNPVLYLR
                     FEEFPLVDSSANSYWVAHNTGVQLSPFETSWGIDNSVYIPNSADNAVAAGPAAAMPAD
                     LTLTGDNYAFADGDITFEFWVNSTGMDAYATIFQQGRTELVAPGIGNSNGTLRILCGD
                     DGNPDNNQNWTYTGAATPLDGKWHHIVLTYDEIDPDHMTIELYKDGAKVSTKAITSTT
                     GQAKLGPELNHLVIGGTGTYTATNPGNRFSGLIDEFAVYAGVLSESRVATHYAAGLAA
                     MKPKTCRQVWIKGQGLTADVDKDCDVDLTDFAAVASGWLLCNDPALFGTDPDCAANW"
     gene            complement(14706..15956)
                     /locus_tag="PLE88_04145"
     CDS             complement(14706..15956)
                     /locus_tag="PLE88_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM75692.1"
                     /translation="MGKMRVWITVLIIAMAAAAPAVEVISIDINNYGNEIAYSGEAAV
                     SGASQWIAYYGGWGVPAGSPRSANLAKTGTIQASTYAEQVWIGDPGGHSYVTGASSGL
                     LDDGFVSNTPSPTFTGNDPNISFIGLDLFGDAGLSHAYKGTFDVYLYGNSAGSFKLTD
                     ANSVSPRLIAAGAVTGTVTPGVFELGKNYVVFENISIADPNSVRLWYSNELNGIQLVG
                     KKMPVQIQKTGTVISAVNYTAAYDTNSRDGEPTRYGPDTGYTAGIGSYVSYLAPGEYM
                     DYDITVDLENMGQYSLTAGVVTASGSASMQIFIDGVLLGAVTCPQYADGMIYETTART
                     INLFPGSHTITWKTPTIRYNNIVDLRLNYSGPVVMSNCAQVRTYGFNYPGDLTGDCRV
                     DMQDLALIIENWANCYDPQPGACD"
     gene            complement(15983..17008)
                     /locus_tag="PLE88_04150"
     CDS             complement(15983..17008)
                     /locus_tag="PLE88_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM75693.1"
                     /translation="MAKKRSVPTVREIASHCGVSVATVSRVLNRKYTNGFSVRQELHN
                     HITKVADELGYRPNLAAKNLVQRQTMTVAILGCNTVFGWPSNIYQNTSEAAIRLLQSR
                     GYNVCITAPNLERDNTELPPWRVDGVLVLQECSPETIDEMERIDVPYVVVNGKGGPSC
                     SSVVPDDIHAARRAVEHLLGLGHRRIAYAGPTPQHRSHASVADRHQTYLSELRIRGLE
                     PIAGHNEQFISGLDYLVSVVLKQKATAIVAYDHVIALKILHDAHILNIDIPRQVSLIC
                     FNDEYLCDMVMPPLTTIGVPSRQMGQIAAEMLLRQMEAAPGERPAEHIRVQQELVVRS
                     STAEPIR"
     gene            17427..19538
                     /locus_tag="PLE88_04155"
     CDS             17427..19538
                     /locus_tag="PLE88_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOM75694.1"
                     /translation="MIEHIHTCRLLYKYNFHTSIKYGLLFVFFTFNSLLYSAVNLLTN
                     PGFEEGTTGWQARNCSISAVTASPRSGLQCGRAYNRTAVWQGIKQSILGKAAPGQTVT
                     ISGWVRLENTASDTIKLTVEQRDDAGTKYIGISQKTVSNSQWTQLTGQFTLAVSGTLT
                     YLDIYFEGPAAGISFYVDDAEVLGPDSSTASCRIDISDRHQIIEGFGAAGAWYEGLLV
                     NHPQKNTLYDLMFNQLGLEIYRIRNTFEIDSAYMDRTAQIIREARALTGRPLKIMISS
                     WSPPAYLKSNNNTREGTLKKNEYNEYMYPEFANWWYRSLVSWASRGIAATYINIQNEP
                     DYVNSGWDTCYLWATETSTMAGYNLAFEAVWQELNGQMGLLMPKMLAPETTGFSNIGN
                     YISNLIDDSHVYGYAHHLYNCSNSTGGAGCGDEPDRYLTTMANFASQYGTKPLFQSEY
                     EDATNAWPDALNLALLLHNSLTVEGVAAYLYWDLFWNSGGLITLTSSGYTLNNDYYGF
                     KHFSAFIHSGWQRLGTVLDIAGLRISAYISPDNQHLSAVIINPGSAAVELTVSIPEFS
                     INSGDIYRSTASQNTEHLGTFNPTDTLSIPAMSITTLSLTGSVIPPDCSKVIAWGFRL
                     PEDLDGNCRVDWSDLLLLAHQWLTETPQPPAPNYSADLTGDNHIDLFDAAALLKQWMV
                     CNEPETVGCIENWQPAILEPH"
     gene            complement(19632..21716)
                     /locus_tag="PLE88_04160"
     CDS             complement(19632..21716)
                     /locus_tag="PLE88_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOM75695.1"
                     /translation="MRSQRWILYWLAVFLGSTAAGEDGYRLWMRYERADSACLLQYHR
                     QATEIVIQGQTETFTVIEKELQQGLSGLLGVAVPSASSVTQDGAIVVGTYQTSPIIQE
                     LFSKSRIDSLGSEGYLLCSVFWKKHPITAVAAKEPIGALYGVFHLLRLMQTQQPLQEL
                     CIHQKPQIQYRLLNHWDNLNGSVERGYAGRSLWQWNRLPSQIEQRYTDYARANASIGI
                     NGTVLNNVNAQAEVLTSQYLQKAAVLADIFRPYGIRVYLSVKFTAPIEIGGLDTADPL
                     RPQVRQWWRQKAEEIYRFIPDFGGFLVKAYSEGQPGPQAYNRTHADGANMLAEVLRPH
                     GGILMWRSFVYDTSIDSDRAKCAYKEFVPLDGQFGPNIFIQTKNGPIDFQPREPFNPL
                     FGAMPKTPLMMELQITQEYTGQAKHLVYLAPQWKEVLFSDTFANGPNTLVKDIVDGSA
                     QGHSISGIAGVSGIGSDRNWTGHHFSQANWYAFGRLAWDMSLSSGQIAEEWIRMTWTN
                     DPQSVSAIRCMMEGSWQACVDYMTPLGLHHLMAEGHHYGPDPGFVHAGRADWSSTYYH
                     RADLEGLGFDRTDKGSNAVSQYHPPLRDWWNCSETCPEKYLLWFHHIPWNQQLSSGRT
                     LWKELQAHYQSGVEYVEWMQRVWESLKGNIDAERYKDVQQRLTVQYENAVQWQNVCLT
                     YFGRFAKGDSDK"
     gene            complement(21769..22977)
                     /locus_tag="PLE88_04165"
     CDS             complement(21769..22977)
                     /locus_tag="PLE88_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOM75696.1"
                     /translation="MKNSRIYWIWAIALMAGFMFSTAGCSAEHQKEAGHSGTAKADYE
                     LPPQADILAAMRLANQYFMDKWPDPGKPIVTDKTRPSNIWTRGTYYEGLMALYEIDPQ
                     KTYYDYAVAWGQAHQWGLRSGSRTRNADDQCCGQTYIDLYRIDPKPERIAQIKECIDA
                     MVSSDKNDDWWWIDALQMAMPVFARLGVLYQDNRYFEKMYQLYIYTKEKHGDNGLYNP
                     QDHLWWRDKDFDPPYREPNGEDCYWSRGNGWVFAALVRVLDIMPSDAPHRDEYIKTFR
                     EMAAALLPIQRPDGFWNVSLHDPTNFGGKELTGTAFFTYGFAWGVRKGMLEEAVYLPA
                     AVRGWNGMAKEALHPNGFLGYVQSTGKQPSDGQPVTYDKVPNFEDYGLGGFLLAGSEI
                     YKLSQQYRSR"
     gene            complement(23005..24171)
                     /locus_tag="PLE88_04170"
     CDS             complement(23005..24171)
                     /locus_tag="PLE88_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOM75697.1"
                     /translation="MKKETMTSRQRVSAALNHQQPDRVPIDLGGFQTGIHVKAYNNLL
                     DYWGLQEKIQIMDPVQQLAKPSETVLQRFHVDIRYITSRSPAGFDGSIKTNRRSGRIW
                     HDLTDEFGVIWSMPDDRPLYMDISYHPLANASITELRHYPFPNGKDPSRFAGLRDEAA
                     RLRQETPYALSTGIGGVIYETCWYLRGLERWYIDMMENPAFCEMLLDKMLAYWMDYYT
                     GFLREVGDLVDIVMLGDDLAGQDGPLFNPEFYRKVVKPRQKKLIQHIKLLTAAKIWYH
                     TCGGCKLYIPDLLDNGIDILNPIQIGAAGMEPAQIKRQFGDKLVFWGGAIDSQHVLPF
                     ASPQQVREEVRRHLEIFKPGGGYVFNNIHNIQAEVPVENIVALYDAAYEYGFYE"
     gene            complement(24209..26914)
                     /locus_tag="PLE88_04175"
     CDS             complement(24209..26914)
                     /locus_tag="PLE88_04175"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOM75698.1"
                     /translation="MGWMFLICSCAAQPTASPWPAMEAQCRPWTYWWWMGSAVEKDSI
                     AFQLEAYQKAGLGGVHIIPIYGVKGYEKQYIEYLSPQWLEMLDYTVRQARQLGMGVDM
                     TLGTGWCFGGPTITNQQANAVVEHAVQTIEAGQSSVKLRSALPLYVAAYGPGQKAVDL
                     TSRLKPDGTLDWTASDGTWQVYELWQKPSGRKVKRAAPGGEGHMLNPFYRPAMEEYLA
                     WFDQAFKDYSGLKPRATYHDSYEYVSNWSPDLLSEFYKRRGYRLEEFLPEFLGYSQDT
                     ERTSRLKGDYRQTLSDLMVENYSLWVQWSHKNGFITRNEAHGSPGNLLDLYALADIPE
                     TEFFRLDRNPLVAKFASSAAHTAGRLRTSSETGTWLKEHFHVTLGHLKNFIDGLFISG
                     INHIFYHGTCYSPRDAEWPGWVFYASTQMNPRNSIWYDVNALNAYITRCQSVLQAGRP
                     DNDLLVYWPISDYWHNPKGMNLDMTVHSTQWLAQQPIGRLAKELWNKGYAFDYISDRQ
                     LQQVYYDTGRIKVPGGDYRVVVVPHCTYIPLDSLQKLFLLAQQGAIILFEKALPQDVP
                     GLGDLERRRAALKALMDSIIWQPQSGLVKTGKVGQGAFYVSEDVASLMNQAGIQRETL
                     VDHKGLYYIRRRDDKGYWYFVTNQDEQFTENPAVSAFAGWITLTRPAQSILIIDPMDA
                     KTGFSPMRQTQDGRTQVYIQLQAGQSLILRLFGEKQNDAVRWTWHQDADSLRLEGSWK
                     VDFITGGPQLPPSFSTTVLESWTKQGQEAQRFAGTARYRLTFDLPQMRADAWRLDLGR
                     VAASARVRLNGQSLGTVFSVPFAVEVPKTLLKSNANQLEIEVTNLSANRIRDLDIRQV
                     PWKYFGDINIVNIDYKKFDASQWEIVESGLLGPVTLIALNEYSPQ"
     gene            complement(27056..30343)
                     /locus_tag="PLE88_04180"
     CDS             complement(27056..30343)
                     /locus_tag="PLE88_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOM75699.1"
                     /translation="MKHGWLVFVAISIVSAGWGKSLSASVSLSGTWRFALDKEGQGIS
                     QEWFKRVLPETIQLPGTLQEQGFGDEVSTATPWMSRLHDKHWYLRVDYERYAQPGNIK
                     IPFWLQPERHYIGWAWYQRDIEIPAVWQGRRVVLTLERPHWSTTVWLDEKQIGFNDGL
                     SAPHIYPLGLVSPGRHRLTICVDNRMLQDIREDAHSITDSTQTNWNGLIGTLSLTSTT
                     PVWIDDVRAFSDIETKTVHLKVWIGNMTGKAGSGTLYVNDKPIPVQWTAEAGYAEADY
                     PLGDSAALWDEFHPSLHRLTLKLTGQQADDQRELTVGLRQIKAEGGRFYLNGRRVFFR
                     GTHEGCQFPLTGYPPTDVESWRKIFKICKDYGLNHMRFHSWCPPEAAFTAADEMGIYL
                     QPECSNWGQYSSRDTRLVEWLERETERIIRAYGNHPSFVLFSTGNEPAGPWQEPLLKW
                     CRDWKARDNRRLYASQTGRFFAERPGPVPDIDYLDTMRIGSYMFRGATGWFGSDFSRS
                     LEGTTYPVISHETGQWCVYPDFSEIPKYTGKLKPKNFEIFRDSLAEHGMLDQAGDFLL
                     ASGKLQAACYKEEIEALLRTPGMGGFQLLDLHDYPGQGTALVGILNVFWESKGYITPS
                     EFRRFCNATVPLARMKRQIYTTAEQLNVDCEIAHFGAQPLQDVQPFWKIVDSGGKEAA
                     GGQFERKTITVENAIPLGTVSISLGSLKAPAKYRLVVGLLGMEIENDWEFWLYPAQLD
                     TAVPQDVVLTRSFEAAAAALQKGQKVLLMPTSDQLQWQSPPIGRLPIFWNRLMGPGWE
                     RFLGLVCQPSHPALAFFPTDEHYDWQWQDVFSPACRAVNIDALPKTLKPIVQMIDDWN
                     RNYKLAAVFECRVGSGRLLVCAADLESNLDRRPAARQLRRSLLDYMGGSHFNPSAEIS
                     LEQLEGLHFDNRIMKKLQAAVTASMQNPGNPASNILDGNPNTYWLAGGRGQGHPYELA
                     ITFPQAVALKGLVWMNRQNHREHEGDVRDYRIEISSDGQNWTLAAQGQLKSTFHPQTI
                     DFGKTTDASRIKIHILSGFGGDSTASVADVAVLYAGPKLADDGSQPSIYQKQKTATEE
                     IFEGPIP"
     gene            complement(30348..32063)
                     /locus_tag="PLE88_04185"
     CDS             complement(30348..32063)
                     /locus_tag="PLE88_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75700.1"
                     /translation="MTFTPEQLNRLFYLFGGVRDGTISRQEFKELDALLAENQQVLDL
                     YWDYVSLCTDLCNLQAATQYNKGISVSLESRLEEPECLQAASEAVSLEMLQLWGEYEK
                     QARAIHLPQEDESEIPVSKIPVEKPIRRVSRSSLLTIAVSLAAILVLLVYIYLHPYPS
                     YEVATVLDAIDAQSASGAAFREGQRISAMSDWIQLDKGVVKIETDRGVQIILEAPAAF
                     QFTSGSEIVLDQGRLLTLITSEMGNGFSVQTTDSKIIDMGTLFGVQADPHRGTELHVF
                     KGKTVLIPSTKSKTKNAVDVSAGQAFSIDRQTSEVKPIALNQEAFVQAIDSKSRFLWR
                     GRHRIDLSDVVGGGSGFGTGRFGMGLNPKNGQFGTVENLIRKSPNTYILIPSSVFIDG
                     VFVPNGQTEQVVSSRGHCFEDCPATSGVYYADISNCPEFIRTEKDGRRYPLVLDGINY
                     SQPGKACIFLHANLGITFDLDAFRSRLPDVRIVCFQADIGVSESVPGSFSADFWVLVD
                     GRVRYKQTDVQTKGRIDTVAIELNENDRFLTLAATEGQIEEENRPSRIGYDWCLFANP
                     ILILE"
     gene            complement(32060..32605)
                     /locus_tag="PLE88_04190"
     CDS             complement(32060..32605)
                     /locus_tag="PLE88_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM75701.1"
                     /translation="MGEETKHGNTRNYQLFIKLYLANERRIYGYIRSFIPNWSDVDDL
                     IQEVASVMWSKFGEFERGTNFSAWALKIAHNQVLNYYKKRKKDKLYFSDQVIEALAEK
                     GIKQMPDVEERLEILRKCLNKLKSHEQTLIQMRYEPGASTKSVSLQTGKELHVLYRLL
                     NKIHAKLLLCIRQMLAEGEPL"
     gene            32782..35487
                     /locus_tag="PLE88_04195"
     CDS             32782..35487
                     /locus_tag="PLE88_04195"
                     /inference="COORDINATES: protein motif:HMM:NF036710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM75702.1"
                     /translation="MRLCAIAIIICLLLSPSMVYAARYMENLDRGTVAVRISSNQVYV
                     GWRMLGTEWGKNIGYHVYRGSTRITSTPITDSTNILDTTTLSSTYSISAVIDGVERPA
                     SPPVAVWDTFYRDIPLQRPAGGTTPDGVTYTYSPNDCSVGDLDGDGQYEIILKWDPSN
                     SKDNSQSGYTGNVYLDAYELDGTLLWRIDLGINIRAGAHYTQFVVYDLDSDGRAELVC
                     KTADGTTDGQGTILGNPTADYRNSSGYILSGPEYLSVFDGQTGAFLDTVDYIPPRGRV
                     SDWGDSYGNRVDRFLACAAYLDGVHPSVVMCRGYYTRAVLAAWDFSGGKLTLRWVFDT
                     NNGYASYRGQGNHNLSVADVDGDGFDEIVYGSCTIDHDGTGLYSTGLSHGDALHVGDL
                     DPSRPGLEVFAIHEPAGETVIGTSFRDAATGEIIWSMTPGDVGRGCADDIYAGNPGAE
                     MWSSNKGGLRDRYGNYLGRSPSSTNFVIWWDSDLIRELLDSNHIDKYGTSSDTRLLTA
                     TNCSSNNGTKSTPCLSADILGDWREEVIWRTSDNTALRIYTTTALTSHRLYTLMHDPQ
                     YRAAIAWQNVAYNQPPHPSFHIGAGMSTPPIPDIVPAGIPVLYGDFNNDAIVDFEDLP
                     YFLDLWLLNSCTLAADADFNQDCMVNLYEFAALAGNWAGSDVTPPAAPAGLTATAGNG
                     TVSLDWSDNSESDLAGYKVYRSIISGSNYTLVSPLLNSSNYIDNDVVNGMTYYYVVTA
                     IDKQSNESQISDEVLAKPDVITSLTIQENEPGFLGIVTGTIDSNHAGFTGTGFANTNN
                     AVGSYIEWAVNTPAAGSYDLRWRFANFGTKDRTGSVSINGSQQAAGISFPITGAWTTW
                     NLTEAVTVYLAEGNNVIRLTAETSEGLANIDWIEITGLWLSAGKS"
     gene            35716..>37014
                     /locus_tag="PLE88_04200"
     CDS             35716..>37014
                     /locus_tag="PLE88_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75703.1"
                     /translation="MKRNMYLAVLLFSVLSLMAYADVTTTVTSAADTGIRDATNTYGA
                     DPYMYIYGAPYHMGYVRFNLAPLSIYTVLNAQLVMTVSGGAPRNDSLVTGRWAVHGLN
                     NVSGNTPQDWNELTLNRSNTGLEVEWTGQTLDIAGGRVTNLDADDGVGTTETIVNGPG
                     GSYAAGTTLTLVDSATGSKPLVNFLQSRVNDNGLVTFIIRQDAGGSRGYGLATKENAT
                     QTYWPYLQITYIPGGAVEPNPADGSTITNLNLSQICWRNYLSDLAVVWFGKGDANEID
                     YKSRLTYLATIEEPANETCLSIPAEMVPLDVPATYTWVVESWKYPATDPNHIGDPNEL
                     MNIKVWKFHTSAIPVVKTNPGEQYKFPGETAVFSATFEALTEMTGAIWQRNGQPLNTS
                     DPDVNITINAAGEHLYTTTLTLSNVSAADDGAYTCIGQNSG"
BASE COUNT         9418 a         9748 c         8766 g         9082 t
ORIGIN      
        1 tcatccgatg gaaggggctg acgggaatac ggatggtacc cctagccccc tgacatttcg
       61 ccgataccaa cgtttcgctg ccggcggtgc tggattgata tgggtagagg ccatcgcagt
      121 ggttccagag ggtcggtcta attcacatca gctctggata cataaagaaa acaaaaaagc
      181 attttccgag ctgatttatc tgattcggag aacagccaac gacagcattg gttcaaaaca
      241 ccgccctgtc atggtagcac agcttacaca ttccggcagg tacagtcgcc ccaaggattg
      301 cccagaacca atgattcttc aatctgatcc ctatcgcgat gcactatgtc cgatgcctgt
      361 tcctgctgct aattatgcaa gtgctctgcc tccagataag actgttttag tagacgatga
      421 atatcttgat aatcttcaat ggaaatatat agaggcggca aaactggcct ttgatgccgg
      481 ctttgatgcg gtggatatca aggcctgtca tggatacctg ataagtgaac ttctatccag
      541 ccgctgccga cagggaaaat acggcactac atttgaaaat cgctcacgct ttctccttga
      601 ggtcatcgac aagattcatc agaatcttgg tgcggattgc ccgatttcct gccggcttgg
      661 cttttacgat gctatcccct atccatacgg ctggggagtc agccacgatg atttctctaa
      721 agccgatctg gatgaaccta aaaaactgat gggactgctc gtgcagcgag gtgttcgact
      781 tttcaatttc acaatcggta atccgtatct gaatccacac cttggccgcc cattcaatcg
      841 ttcggccaca gatgggtata tctcacctga acatccgctt aaaggcgttg agcgaatgat
      901 tacactggcc ggtgagctgc aaaagtgtta tcctgatgct gtttttgttg gaacgggcta
      961 tagctggctt ggtccgatga tgggatacgc aggagcagct gcaaaagcat tgggaaaaag
     1021 taagatgata ggtgtcgggc ggatggcttt tgcctatccg gattttgcta aagacctgct
     1081 aaacaagggg actttggaag agaataaagt ctgtattgcc tgcagcggat gttcgcagct
     1141 gctgcgccgc ggtctgccga ccggatgtat tatcagggac tcgagctttt atgggaaaga
     1201 gaaaaaaagc aaatccccaa ccagctgata acagctcccc ccttccgcct gcaggtatcc
     1261 gcagacattg ggcagcgtcg gaagcggcag caggcgcagg attggcgtta tttcttttta
     1321 cgatttacac cgcaactatg ctgcagggtg tggatgcagg ggattcggcc gaactgcagt
     1381 ttatgagtcc tctgttaggg gtgtgtcatc cgccggggta tgctattgaa gtatgctttg
     1441 gcaagttgtt cagcatgctg cctttcggac cctccgcagc atggcggatc aattttatga
     1501 tggttatcag cggcacagcg ggctgtcttg cgctgtatgg aaccatccgg cgaatcacag
     1561 gaagcatcat agctggagtg gttggggcac tgacgctggg attaagcagt atttactggt
     1621 ccttcagtgt ggcagcagaa gtatatgttt tttatgcgat gtttctgctt tgggggctct
     1681 attgcattgt ccggttcatt gacagcaaac gttctgtttg gctgtatgcg gcagcggcag
     1741 ttcttgggat atgtacagca gacagaatat cagagatact gataatgccg ggatttttac
     1801 tgctgtgggc ttccgtgcga aaagaagtta aactcagcat tctgcaattg ctggccgctg
     1861 ccatcgtgtt tattcttcct tttctcttta cagtaagttt tcatcttatc caaaatcatc
     1921 cccaaaaaag aaccgaggcc ttatacggac gggactcggc acttcgattt cagattttgg
     1981 ccgatcattt cgaggtttat atccctcctc tgcttcagcg catcaaaggg gctgtacagt
     2041 actgtctggg gctgatgtac aaaaaagatg ccaagtttaa tacctcagtg atggcgtggg
     2101 atatgaataa gtatgtttgg ctgctaagcg gtatgggagc agcaggcgaa cgatttccgt
     2161 caggggacag gcgaaacagc gaacaaggaa ccggcacgag tatcggaata ctcggattag
     2221 ccgccgcagc agccggatta attcagcacc ggaaaaactg gggatggctg ctgcttggat
     2281 tatggatgtt ttcggccaat ctcatcttta tcctctggca tcaccgctgg gataacctta
     2341 catttacaat ccccggcctt gctggattgg cgatgctggc aggaataggt gcctcaggcg
     2401 gacaagaagc gggcagaaaa acagttattt atcggtacat ttgtttgatt attccgcttt
     2461 ttctgctggt tacaaattac ccgttagtca accgcaatac agccgcagaa aagaagaatt
     2521 tggagcagtt gcagaaaatt tccaaggcct cttttgccgc caacagcgca atcataacaa
     2581 cctattggcc cgctatgact tatcgttact taatacagct cgaaggagga cgagaggata
     2641 tacggattat tcatgaagaa acaaaaaact ggcaaaaact cgtggattat ttcctcaaca
     2701 agggcctgcc ggtctatatg gaaaaaaaca ggatgaatcc cgatatactt cagcaagccc
     2761 agaagcaaac agagacggac gtatggcagg caggatttgt gaagctgccg caattggaac
     2821 cgcatcggaa ttgacatgct ttcaaaacag aattattatt cctccccttc taaaacgaat
     2881 cggactataa acctttttag aaatccttgc aaattttggt tatttagggt ataagattaa
     2941 attacacagc agtaatacta tataggtgct ggattaaaaa gataacagat gcataattta
     3001 tggaattagc agaacttcgt acatcaattg gtgatttaga ggcccggatt gaacaagtcc
     3061 gggactggct ttgacgtgcc tgccaaaatt gcgcttcgtg ataaattaga gaagcaaatt
     3121 gcacaacctc acttctggga caatcctgaa accgcccgtc aggtcgttac gcagcttagt
     3181 accgttaaat cggtgattgc gcctattcag gaagccgaaa aaacccttca ggacattcat
     3241 gagctgtttg tattagcaag tgaagaagga gacgaggcaa cattggcttc gatacaggcc
     3301 gatttggaaa aactggctgc tcaatgcgat aaaatcgaaa tagccggaat gctcaatggt
     3361 ccggatgatt cacgcccctg ctttttcgga attcacgctg gagccggagg gactgaaagc
     3421 tgtgattggg cggcaatgct gctgcggatg tatacacgtt attttgacga caacaagtat
     3481 cggtatgagc agcttgatct tacgccgggc gaggaagccg gtattcgctc gattaccctg
     3541 cgggtcgaag ggccgtttgc cttcggcaag ctttcctgcg aaacgggtgt ccatcggctg
     3601 gttcgcatca gtccgttcga tgcggccgga cgccggcata caagctttgc cgcagtggat
     3661 gtgatgccgg agtatgaaga cgacatcgaa atagacatta atgaagagga cctgcggatt
     3721 gactactacc gcgccggagg agctggcggt cagcatgtca ataaaaccag ctccgccgtc
     3781 cggataacac atctgccaac caatattgtc gtccaatgtc aaaatgaccg cagtcagcat
     3841 aaaaacaagg cagaagcaat gaaactgctc aagagcagat tgtatatgct tgagcagcag
     3901 aagcgcgatg cagaactggc taaactttat tccagtaaag gcgatatcgc ctggggcaat
     3961 cagatacgaa gttacgtact ccagccgtat cagatgatca aagaccatcg tacagactat
     4021 cagagcggca acgtacaggc agttctggac ggggatatcg aaccgttcat cgagagctac
     4081 cttcgctatc gggcacagaa aaaacataaa ccttcatgaa aacgcatcca caaaaactgt
     4141 attcacggcg tcactttctt aaaacagctg ctgcgagtgc ggcagctgtt tacgcaggct
     4201 gctgttcaac ccagcaaatc tgccgccgac agtcagcagg taaacccaat atcatttaca
     4261 tcctcgccga tgacctcgga tatggcgatt taagctgtta tggtcaaaag cggttcagca
     4321 ctccccatat tgaccgattg gctgacgaag gtcttctctt tacccggcat tatgccggca
     4381 gcacagtctg tgctccgtcg cggtgttcgc taatgaccgg actgcatacc ggccactgcc
     4441 agattcgggg caacctgagt gttgagccgg aaggccaatg gccgctggca gcaggaacaa
     4501 aaacggcggc ctctgttctg aaggaggccg gctatagtac cggtgctttc ggtaaatggg
     4561 ggctgggagg acctgacagt tcaggcgacc cgaatcgtca gggctttgat gaatttttcg
     4621 gctttaaatg ccagacaatg gctcataaat actaccagcg gtatctttgg cgaaataatg
     4681 aaaaggttta cctgcccggc aacgaccctg aaaagcaaac cggccaatat tgtcatgact
     4741 taatcgttga ccaggcaatt gaatttatta aaacgcacca caagaggcct ttttttgcct
     4801 atttggctct gactatcccg catgccgaac tggcagttcc tgcagactcg ctcgagaaat
     4861 acaaagggct gtttccagaa gacaatccgt ttattcccaa tcccaatcag gattatggac
     4921 gtcagctcca tccccgggcg gcctatgcgg caatgattga ccggatggac cgagatgtag
     4981 gacggatttt aaccctgctg aaagaactgc atattgatga caataccatt gtcttcttca
     5041 gcagcgacaa cgggccgcac aaagaaggcg gcggagatcc agactttttt gacagcaatg
     5101 ggcctttgcg gggatataag cgggatttat acgaaggcgg tattcgtgta ccgctgctgg
     5161 ttcgctggcc cagcgtcatc aaagccggaa cccgcacaga tcatatcagc gcattttggg
     5221 atacattgcc gacatttgct cagctggcag gaacacaaag ctttcctgac agtgacggca
     5281 tttcatttct gcctgtgctg ctgggcaatc cagatgcaca acttcggcat ccttatctgt
     5341 attgggaatt tcacgagcgc gggggcaaac aagccgtcct tattgggaat cagaaggccg
     5401 ttcgcctgaa tgcgatgaac aatccggatg ctcccttgga attgtatgat ttagctgagg
     5461 atccgtcgga agaaaacaat attgcagctg cgcatccgca attaattgaa aaaatgactt
     5521 ttcttatcca tcaagcacac aagccaaaca aaacatttcc tttctttgca tctgaaagaa
     5581 acagctcgtt acagagataa taaatttaat gaaagaccga accaatgact aaacagttaa
     5641 tagacattgc agttggggta caaaagaccg acatctttac ctgtgctgcc gatatagcag
     5701 cggtgggagt ttttgaggat aattactcag gacatctgat aaaagaactt gataaacggt
     5761 tgggcgggca gatcaaattc atagaaaagc ttggggattt tgacgggaaa gctgcaagcc
     5821 tttgtctgct gtatacaaat ggcaagttag ctgctaaacg gcttcttctg gtcggcttag
     5881 gcaagcgcag taagctgacg gctgctgtgc tgcacaaatg cgctgcagca gctgccggca
     5941 aagcggtcga gataaatgcc ggctcggccg tattctgtct gcattcagat gccgctgcga
     6001 agctggcaat ggaagatatt ggtcagattc tgactgaagg agcaatgttc ggagcttttc
     6061 gctatgatga atatataaca aatcccgaaa agaaacgtcc tgcgcgcctg aaaatcattt
     6121 tagccgatag cagccctgct gtcgtcaagc agctcaaaca aggagccgct gccggcatca
     6181 tcacaggttc ggcccaaaac tatgcccgtg cattcgccaa ccgccccgcc aatgtgatta
     6241 cccctgccgt gctggctgca gaagccaaaa ggctttccca aatcagttca cggctgcgct
     6301 gtacaatatt caaccaaagg cagctgcaga caaaaaaaat ggaaggtatt ttagctgtgg
     6361 ggcaaggttc caacaatcct cctcgtctga ttgtattaga atacacaccc gcgggagcat
     6421 cagggcgcct ttcttcggtt gcgctggtcg gcaaagcgat aacatttgac tcaggcggta
     6481 tttcactcaa accgggcgaa ggaatgcagg atatgaaatt tgacaaatcc ggcggattgg
     6541 cggttctcgg cgcgatgagg gctatcgctg ccttacagcc caaagtgcgt gttttcggca
     6601 tcattccagc tgccgaaaat atgccgtccg gcagaagtta tcgtccgggt gatattataa
     6661 ccacatacag cggcaaaact gtagaaatcc acagcacgga cgcagagggc cgtatgattt
     6721 tgtgcgatgc tctggcgtat gccgccaata cgctgggctg tcctgttatc gttgatatag
     6781 ccacactgac cggcgcctgt gttgtagcat tgggacagaa gcgggcgggt ttgctgggca
     6841 atgatgatga gctgatggaa caactgaagg ccgcctcggc caaaaccggt gagcggattt
     6901 ggccgctgcc ctgtgatgac gaatatctgg aagcgatgaa aagcaaaatc gctgacttga
     6961 aaaataccgg cggcaaatgg ggtggggcct gtacggcagg ggccttttta agccagtttg
     7021 taggcaaagc caaatgggca catattgata tggccggcgt cggcgtttgg ggaggcgcag
     7081 aaggcgagac ggccggctcc atcggctttg gagtgcggct tctgacagaa tttgttcgga
     7141 attacaaata gcggcttctt atccgaaaac gctgcttatc cgctgactgt cagaatcact
     7201 ttcttaagat cgcagtcacg ggaagaattg ccgaccatta tctcaaaagc ccccggctca
     7261 acaatatagt ccatatcaat gttataaaac gccaaatgtt ccggctcaat atcaaaatgc
     7321 acagtgcggg tctgtcccgg agccagagaa atccgctgga aacccttcag ttctttgacc
     7381 ggacgggtta cagtactgta acagtcgcgt atatacattt gcacgacttc gtcgccttcc
     7441 cggctgcccg tattggtcac atcaacacag acagtagttg attcgtccgg acgaattgtc
     7501 tgtttttgga gccgaagatt ggtaaatgcg aattgcgtat agctgaggcc gaatccaaat
     7561 gcatacagag ggcttacaga atcaaacagg tatccgcggc gggcagaagg tttataatta
     7621 taataagcag gaatatgtcc gacagagcgg ggaaaagaaa tcggcagctt gccgccggga
     7681 ttgagctgcc caaacaaggc ctccgctacc gctggcccgg tttcctgccc cagataccag
     7741 cactcaaaaa taacaggaac cttttcggcc agatgacgaa tatccagcgg ccgaccgtta
     7801 aagagaaccg caataaccgg cttgccggaa gccgctaccg catcgactag ttcatcctgc
     7861 aaacctacca ttcgcaggtc tgcccgatcg cccatatggt ttttatccca tgcctcacgc
     7921 gaggtctgtt cgttgccgcc gatgacaagc acgacagcat ccgcctgccg ggcgattttt
     7981 gctgcttcgg caatcgactt gcggtcttcg gctggatcac tcaaaatcac ttcatcctgc
     8041 tgccaagaac cgccgacggt gattttgcag ccttcgtgat aaagaatttc tgcagaattg
     8101 cccaaaaagt cacggagacc ctgcaggaca gtcgtataac gcggcggagt tccgctgtaa
     8161 ccgcccagca taacgcgatc cgcattgggt ccgattacgg ccagtgtttt aatagccgat
     8221 gcgttcaacg gtgccactgc gccgtcattt ttaagcagcg taacagtttt tctggctgct
     8281 tccatcgcca gacggctgtt ttcttcacac ccgacaattc gttctgcggc atccggatcg
     8341 acccacggat cttcgaacaa tcccagcctg aatttccact caagcattgg cgcaacaaga
     8401 ttatccaaaa gtgattctgg aatgcttcct tccttgacaa gctgaatcag gtgcggatag
     8461 caatccggat ccggcagttc aatattcacg ccggctctga cagccagcgc tgcggcctgt
     8521 ttgggatttt cggctaaata ctgaccgaaa aagccgggcc tctcgttaag ctcccgtatc
     8581 gcataataat ccgacactac atacccttta aagccccatt ccttcctcag gacatcctgc
     8641 agcaaccatt tgttggcatg ggaaggcaca ctgtcaattt cattatagga agccataaca
     8701 ctaatagcag gcccttcttg cacggccttt ttgaaaggat ataaaaaaac ttcacgcagg
     8761 atacactggg atacattaac cggagcacag ttggttcccg attcaggctg gccgtgggcg
     8821 gcaaagtgtt tgagcgttgc tatcagatgt tttttatcct taaaagtccc atccccctga
     8881 aatccccgaa cagctgcaag ccccatttgg gctgccagat agggatcttc accaaaggtt
     8941 tcttcaaccc tcccccatcg cggctctcgc gccacatcca gaaccggagt caaggcctga
     9001 tgggctcccc caagacgggt ttcttcggct gtcatttgat aaagccgcat aacaagctcc
     9061 ggatcgaacg tggctgctaa tcctatcggc tgcgggaagc tggttgcccc taaggcagca
     9121 tgcccgtgaa ggcactcctc atgaatgaca acaggtatgc caagtcgggt atttttccgc
     9181 atatattcct gaatagcatt ggccatctgg gctgttttat aagcagttaa gcccccggcc
     9241 gcatcgctga gccgccctat ctggccgagg ccgttttgca agttgagttt gctgctttgc
     9301 ggattaaaat gtcctttttc atcgaaaaaa gtcccacact tgctctgcca tacacacagc
     9361 atctgcgcga ctttttcttc caacgtcatc cgcgaaagaa gatcttgtac ccgcagttca
     9421 accggtaaat caggattcca atacggatgc ttcttgttct ctgtcttatt catcggcact
     9481 ttttcaatcc ttttcttttc gtgttgttct atttgatgtt ctcaaaaaat ggtctgttca
     9541 attgcctgca gagtttttct gccggcgctc atttaattgt gactctatcg ttttcatccg
     9601 ctggttattc aatggataaa acagcataac agccgcacct aacagagcaa aaataccagc
     9661 atacacactc attaccatgc gaatgcctgt aagggcctgc ggggtctgcg gctgattggc
     9721 aacatagccg taagcagcca taatccatgc ctgtgctgtc ccgcccagcg ctacacccat
     9781 tttcaaggcg aaaagggatg cagccataat caatgcagtc gcccgccgat tggttttcca
     9841 ttcggaataa tcggctgtat cggtataaat agcccactgc agaaccgata ccggtccgat
     9901 ggcaaatgac gtaataaact gcagcacgaa gagaagaaca atatcgtccg gccccagcac
     9961 aaacaccagt gcactggcga tccctgctat ggccaatccg ccggaataag tctgggattt
    10021 tccaaaagcc cgggcaatcc atttggtcag aacagccccc agaatggtaa aaactgttcc
    10081 tgtcagcaga taagccgaaa cgatatcctc aaaggagtag acttttgttt tgccgaaaaa
    10141 tgacaattcc ttattgccaa ggtagtattc aaaataatac ataatactgc tgcttcggat
    10201 cacaatgaac atcaattgga aaaccgtggc gctgccaatc agcagccatg gcttattggt
    10261 cagaagatcc ttaagatcat cggccagttt attacgctgt tggccgatgg gctgcacgcg
    10321 ttcgcgggta gtaaaaaaag taatcagaaa caacaccacc gccagacctg caagggctgc
    10381 cgcagccagc tgccagccaa gctgctcatt gcctccgccg aacagcttca ccagtcgaag
    10441 cagggcatac tgcacaataa acattccgac aaacgccaga acaaaacgga atgacgaaac
    10501 aaccgttcgc tccatagagt caggactgat aacccccatc agcgctgcat acgggacgtt
    10561 aacggcggtg taaatcattg tcatagcaat gtacgttaca taggcgtaga tcagtttgcc
    10621 ggtcgggcta aactggggtg ttgtaaacat cagtacaccc aatatgccga acggcaccgc
    10681 accaaataaa agatagggtc gaaacttgcc ccatcgtgaa accgtacggt cggcaagcat
    10741 gcccataatg ggatcgttga ctgcatccca aaaacgggtc actagcagca ttaagccaag
    10801 ctgggctgct gttatgccga acacatccgt ataaaagtaa agcagaaaaa taatgaaaat
    10861 ttggaaaaac aggttggaag ctgtatcccc aagaccgtaa ccgatttttt caaaaataga
    10921 tagcttctgt gcgttcgatt ccattgctga ttcctttaaa aaaacagatc tttagacagg
    10981 tttatctgcg ggtaatgtct ttatccagtc tccatactga taaaaaatgc cttttacaga
    11041 ttgttctgaa ggtatttttg gggattgacc acccaccggc cgggctgttt ggggctggcc
    11101 tcaaaatcct tgataataaa aatttcctct ttcttgttgt actcggcatg gccgtcataa
    11161 aaaccgatat tggccccctc gctgtggcgg tagatagtag gaccgtgcaa tccgacatct
    11221 ttataggcct gaatattggc ctgccccagc acatcccaac ccttgcgata gtccgcagca
    11281 gaccactgaa cccaccagtc attagagtca ataaaagcga tgcgtgtcgc aggagaggta
    11341 atgttgctca ttttgtggcc ggcataatca accatatccc atccccagtc ggtataatta
    11401 tagccgtagc tgattaatac accctgtact gcattggcgg gatctttgcc gattttatcg
    11461 gtcgggcaca gaaacttctc aggcgattta tatttcatcg tcgaattgct ttgaaaagaa
    11521 tccaagtcca gattggcacg aaaggacata ttggtaatcc agtggttctg aaaatcctta
    11581 cggtttctgt ctaaaatcgg cacataagct ccattatgct gattggcata caattcgttg
    11641 gcgattccca gtgacttgag attattaatg catacggttc gctgggccgc ttctttggct
    11701 ctcttcagtg caggcatcat aatcgacagc agtagagcaa taattgctat caccaccagc
    11761 agctcaatca aggtaaatcc tattttcttt ctcatgtctt tttccttcca aacatttatc
    11821 ctttaattct ccctgctgct atccgattat acacaccccc tcccccttgc agcggcccgc
    11881 tggacagcgg gccgctgcta cttctgttcc ttttccctac acgctgccaa ttttaaaaca
    11941 ggactacttc ttccgcaata aggcaattgc acccaatccc agcagggtta acgtagccgg
    12001 ctcaggtatt atacctgcgg cgtagtgagc tgcaacacgg tcagcgctta gtacagtatc
    12061 ataaattgcc acttcgtcga gcaggccgac aaaaccattg tacaaatacc atcgattgcc
    12121 ttcactgcca agggtcagac ggtcgtaact aaagcccaac gccccgtctt cgataacatt
    12181 gccccatttg ccgacaccgt caatatagac atactcgtac atatcgtcta tgccatcttc
    12241 attatccaga agcggattgt aggtcacaac gatatgatgc cattcgccgt caaagacatt
    12301 gcccgtgccg gtgtaccatg tagttccggc tccaatcacc gtgatgtctc cggtcgtcga
    12361 accataggac ccaatcccgt aaccatccaa ggtattatag gcgccgttgt gctgcataat
    12421 gcgggcatag ccgcccgtgg tgctgctctt gatccaagcc tcaagcgaaa ccgtctgaag
    12481 aggatagccg aagctgtcct tggacaagga atagggatag gcagcaccat cccagatatc
    12541 aatgcagttg ccggcaccgc cgctgcctga accgtttaac tgcgccgcct ggccgacctt
    12601 tccgggtacc agagcaatat ccgcaacgcc tgtaccatta gtaatgtagg tgccattttt
    12661 ataggcacgg gttgccgaat cgaggcagac atcgccgttg gcactggaag catcctcgaa
    12721 ctcccaaaac gacaagggat tgtctgccat tacaacatcc ctgtaggcag ccggcgatga
    12781 aacgctcatc gctgcgcaca aaacacccaa aacaatcttt gctgcattca gtttcttcat
    12841 cgttacatcc tcccaaccaa aaaattaatg cctgcctcgc acgccgagac agattctgcc
    12901 gcatttgttt ttatctatct ggcaatcttt attctgcttg atgctgccgc ttaccaattt
    12961 gcagcacagt ctggatccgt cccaaacaac gcaggatcat tgcacagcag ccagccggaa
    13021 gccaccgccg caaaatcggt caaatccaca tcacagtcct tgtctacatc ggcagtcagc
    13081 ccttgccctt taatccacac ctgccggcag gtcttgggct tcattgccgc cagtcccgct
    13141 gcgtagtgtg tggccacacg actttccgaa agcacgccgg cgtacactgc aaattcatca
    13201 atcagacccg agaagcgatt gccgggattc gttgctgtat aggtacccgt gccgccgata
    13261 acaaggtgat tcagctcggg ccctaatttg gcctgaccgg ttgtactggt aatcgctttg
    13321 gtcgatactt tagccccatc cttatacagt tcaattgtca tatgatccgg atcaatctca
    13381 tcatatgtca gaacaatatg atgccatttg ccgtccaagg gtgtagctgc accggtataa
    13441 gtccagttct gattgttatc gggatttccg tcatcgccgc ataaaatccg caaagtacca
    13501 ttgctgttcc cgatgcccgg agccaccagt tctgtcctgc cctgctgaaa aatagtagca
    13561 taagcatcca tccctgtgct gtttacccag aactcaaatg tgatatcgcc atcagcaaaa
    13621 gcatagttgt cgccagtgag cgtcaaatct gccggcattg ccgctgccgg ccctgccgct
    13681 acggcattat cagcgctgtt gggaatatag accgaattgt cgattcccca cgaggtctca
    13741 aagggactca gctggacgcc cgtattatgt gcaacccagt agctgttggc agatgaatcc
    13801 acaaggggaa actcttcaaa ccgcagatac agcaccggat tatcatccat cacttcttcc
    13861 acataggatt tatgtgtaat ctgctcaatc gtcgaatcgt atgtaacgac cagatgatgc
    13921 caattgccgt ctgcaatgga tacgcctccg gacgtatcga aataatttgt cgttccgcca
    13981 ccgattacac caatagtgtt tgcatcaacc agcataacac catagctgct ggggctgtcc
    14041 catccgccat tatgctggaa caaccgcgga taagtattag gatctgctgt gatctgcatc
    14101 cataattcaa tcgtaatatc ctccagagaa aaagcccccg atgcatccga gacatccacg
    14161 caatcccctg ctccatcggg agcatcgccg tgaaatacgg ctgctttgcc cgcaacaccg
    14221 gccgaaaggg atacactgtc aatgtaggta ccgtttacgt tggcagttcc gctgtttttg
    14281 gcggggctgt tattgaaact gttggggtcc tcaaaccgca gatacagcaa aggattgtcg
    14341 ctgagtacct gattgacata agcattggga tcggaataca gcgcattgta atgagcggta
    14401 atccgcaaac tatttaagat gccggcgtac accgcaaatt catcaatctt gccgttgtat
    14461 tcgttatagc gatagccaac gcccccctct gctccgataa taagacggtc ataggcatag
    14521 accagcgcgg cttcggctgt cgtctgaccc atataaacac cgtccagata aagggtcttc
    14581 accacattgt ctgcccgcgc aaaaccgctc agcagcagaa caagccctaa catatattga
    14641 gatctcatat tctttctcct cataccgatc gtttcctgat accttgttat ccagcttggg
    14701 cgaatttaat cgcatgcacc gggctgggga tcatagcagt tagcccaatt ttcaatgatc
    14761 aatgccagat cttgcatatc gacccggcaa tctcccgtca ggtcgccggg ataattgaat
    14821 ccgtacgtcc gtacttgggc acaattgctc attacaacag gcccgctgta attgagtctg
    14881 aggtccacaa tattgttata gcggattgtc ggtgttttcc acgttattgt gtgactgccc
    14941 gggaaaagat tgatcgtacg tgctgttgtc tcatagatca tcccatcggc atactgggga
    15001 caggtgacag ctcccaacaa taccccatca ataaatatct gcatagaagc ggaacctgag
    15061 gcggtaacaa cacctgcagt gagactgtac tgccccatat tctcaagatc aaccgttata
    15121 tcataatcca tatattctcc cggagccaaa tacgagacat aacttccgat tcctgccgta
    15181 tatcctgtat ccggcccata acgggtaggt tctccatctc ggctattggt atcataggct
    15241 gccgtataat tgacggcact gataacggtg cctgttttct gaatctgcac cggcattttc
    15301 ttgccgacaa gctggatgcc gttcagctca ttggaatacc acagccggac actgttgggg
    15361 tcagcaatcg atatattttc aaacacaaca taatttttgc ctaattcgaa taccccgggg
    15421 gtgactgttc cggttacagc tcctgccgct ataagacgag ggctgacgct gttggcatcc
    15481 gtcagtttaa agcttcctgc gctgttgccg tatagataaa catcaaatgt gcccttgtag
    15541 gcatggctca gaccggcatc gccaaacaga tccagaccaa taaaggatat attcggatcg
    15601 tttcccgtaa acgtgggaga aggcgtattg cttacaaaac cgtcatccaa taaaccgctg
    15661 cttgcgccgg ttacatagct gtgtccgccg gggtccccga tccaaacctg ctcggcataa
    15721 gtgctggcct gaatcgttcc ggttttagca agattggcac ttcggggcga accagccggc
    15781 actccccagc ccccgtaata ggcaatccat tgagatgcac ccgaaaccgc cgcctcgccg
    15841 gaataggcga tttcattgcc gtaattattg atgtcgatgc tgataacctc aacagcaggg
    15901 gcagcagcag ccatagcgat tatcagaact gttatccaaa ctctcatctt tcccataata
    15961 ttgcctccag taaattgcct ttttatcgaa ttggctctgc cgtggacgaa cgaacaacca
    16021 gctcctgctg cacgcgaata tgttcggcag gccgttcgcc gggagccgct tccatctgcc
    16081 gaagaagcat ttcggcagcg atttgcccca tttgccgtga cggcactccg atggttgtca
    16141 gcggcggcat taccatgtca cacagatatt catcgttaaa acagattaaa ctaacctgcc
    16201 ggggaatatc gatattcaag atatgggcat cgtgcaggat tttcagggca ataacatggt
    16261 cgtaggccac aatagccgta gctttctgct taagaacaac cgataccaga taatccaatc
    16321 cggaaataaa ctgttcatta tgcccggcga tgggctccaa tccgcgaatc ctcaattcag
    16381 aaagataggt ttgatgacga tcggccacac tggcatggct gcggtgctgg ggtgttggac
    16441 ctgcataggc aatccgccga tgccccaatc ccagaagatg ctccactgca cgccgggccg
    16501 catggatatc gtccggcaca accgatgaac aactgggacc ccctttcccg ttgacaacaa
    16561 catagggcac atcaatccgt tccatctcat cgatagtctc aggagaacac tcctgcagta
    16621 caagcacgcc gtccacacgc caaggcggca attctgtatt atctctttcc agatttggcg
    16681 ctgttataca gacattgtag ccccggcttt gtagaagacg tatagccgcc tcactggtat
    16741 tctgataaat attgcttggc cagccgaaaa cagtattgca acccaggata gccactgtca
    16801 tcgtctgacg ctggacaagg tttttagcgg caagatttgg acggtagcca agctcatcgg
    16861 ctacttttgt gatgtgatta tgcagctctt ggcgaacgga aaaaccattg gtatatttgc
    16921 ggtttagaac gcggctgacc gtagcaacac ttacaccgca gtgggatgct atttcccgaa
    16981 cggtaggtac cgaccgtttc ttggccatcc ttcctctcct cgtcagcatg aaaagcatca
    17041 gtaaacgttt acttatagct tattctctca tatcataagc tgtcaagaaa taatcttata
    17101 aatcacaatt ttgtcctaat tattcacccg aactggaatc cttgtgaata tttataagta
    17161 tttattttaa aagattttaa agcaaaatct aaaaactgtt ctgcacgcat tgtaattttg
    17221 ttttggtaaa atatttttat cggactttgt gtaaaaaaag cctgatattg gggttatttc
    17281 gacgtaaata gaaggtatgc ggtaaatcac attgacaaca ctggtctttt ctaaataaaa
    17341 taagttatat cctcagtaaa cgtttactga acaaagaaga ggcaaaataa ttgcctgaat
    17401 ttctcatata agggggcaaa agaaaagtga tagaacacat ccatacttgc cgtttattgt
    17461 acaaatataa ttttcacaca tctatcaaat acggactttt atttgtcttt tttaccttta
    17521 attctctcct ttacagtgct gtcaatttgt taaccaatcc cggctttgaa gaaggcacaa
    17581 ccggctggca agccagaaac tgctccattt ctgcggtcac cgcctccccc cgcagcggct
    17641 tgcaatgcgg ccgtgcctat aaccgaacag ccgtctggca aggcatcaaa caatcgatac
    17701 tcggcaaagc agcacccgga caaaccgtaa ccatctcggg ctgggtacgg cttgaaaata
    17761 ccgccagcga caccattaaa ttgacggttg agcagcggga cgatgcgggc acaaaatata
    17821 tcgggatttc ccagaaaaca gtcagtaata gccaatggac ccaattaaca gggcaattta
    17881 cgctggctgt ttctggcaca ttgacttatc tggatatcta ttttgaaggt cctgcagccg
    17941 gaatcagttt ctatgtcgat gacgccgaag ttcttggacc agattccagt acagcaagct
    18001 gtcgcattga tatctctgat cgtcatcaga ttattgaagg attcggtgct gccggcgcat
    18061 ggtatgaagg cctgctggtc aatcatcccc agaaaaacac tttgtatgat ttgatgttta
    18121 atcagcttgg attggagatt tatcgaatcc ggaatacctt tgaaatcgat tcagcatata
    18181 tggaccgtac cgctcaaatc attcgggaag cacgggctct taccggaagg ccgttgaaaa
    18241 ttatgatttc ttcatggtct ccgccggcct atttgaaaag caacaacaac acccgtgaag
    18301 gaacgctgaa aaaaaatgag tacaacgagt atatgtatcc ggaattcgca aattggtggt
    18361 atagaagtct ggttagttgg gcttcccggg gaattgcagc aacttatatt aatatccaga
    18421 acgagccgga ctatgtcaat tccgggtggg acacctgcta tttatgggcg acagagacct
    18481 cgacaatggc cggctataat ttggcttttg aagcggtctg gcaggagctg aatgggcaga
    18541 tggggctttt gatgccgaag atgctggccc cggaaacaac cggattttca aatattggga
    18601 attatatcag caatcttatc gatgattccc atgtctacgg ctatgctcac catctgtaca
    18661 actgcagcaa cagtactggc ggtgccggct gcggagatga gccggaccgc tatctaacga
    18721 ccatggctaa tttcgcctct caatacggca ccaaacccct gtttcagtcg gaatatgagg
    18781 atgcaaccaa tgcttggccg gatgccctga atttagccct gctgctgcac aattctctga
    18841 cagttgaagg ggttgcagct tacttgtatt gggatttatt ctggaacagc ggaggtctga
    18901 ttaccttaac ttcatccggc tatactctca ataacgatta ttatggattc aagcattttt
    18961 ctgcgtttat tcactccggc tggcagcggc tgggaaccgt tctggatatt gccgggctgc
    19021 ggatatctgc ctacatcagt cccgataatc agcatctgtc agcagttatt atcaatcccg
    19081 gctctgctgc cgtcgagctg actgtttcta tccctgagtt ctcaatcaac agcggcgaca
    19141 tttatcgcag tacggcctcg cagaatactg aacatctggg aacatttaat ccaaccgata
    19201 cattgagtat cccagcaatg tctatcacta ctctgtcctt aaccggctcg gttattcccc
    19261 ctgattgcag caaggtcatc gcatggggtt tccgcctgcc ggaagacctt gatggaaact
    19321 gccgtgtcga ttggagcgat ttgctgctgc tggctcacca atggctgact gaaacgccgc
    19381 agccgccggc acccaactac tcggctgatt taacagggga taatcatatc gacctgttcg
    19441 atgcagcagc cctgctcaag cagtggatgg tttgcaatga gccggaaact gtcggatgca
    19501 tagagaattg gcagccggct attctggagc cccattaaat aagacagttt tactatctca
    19561 atgccgccaa caatcttcaa gacccatcaa gaactccgca agccggacgg gggcgattgc
    19621 ttaccaatca ttcacttgtc tgaatctccc tttgcaaaac gcccgaagta agtcagacat
    19681 acattctgcc attggacagc attttcgtac tgtactgtca gacgctgctg tacatctttg
    19741 taacgttcgg catctatatt ccccttgagt gattcccaga cacgctgcat ccattcgaca
    19801 tattctactc cgctttggta atgggcctgc aactctttcc acagcgtgcg tcccgagcta
    19861 agctgctgat tccatggaat atgatgaaac cacaggaggt atttttccgg gcaggtttcg
    19921 gagcaattcc accaatcccg cagcggcgga tgatactggc tgaccgcatt gctgcctttg
    19981 tcggtgcggt caaagccgag tccttccaag tcggcacgat gataatacgt ggaactccaa
    20041 tctgccctgc cggcgtgcac aaatcccgga tccggaccgt agtgatgccc ttcggccatc
    20101 aaatgatgca accccaacgg agtcatataa tcgacacacg cttgccagga accttccatc
    20161 atacagcgta tcgctgatac agattgcggg tcattggtcc aggtcatccg aatccattct
    20221 tcggcaattt gcccgcttga cagcgacata tcccaagcca agcgtccaaa agcataccag
    20281 ttagcctgtg agaaatgatg gcccgtccag ttgcggtcgc tgccgattcc gctgacacca
    20341 gctatgccgc tgatagaatg gccttgggca ctgccgtcaa caatatcctt caccagagtg
    20401 ttgggaccgt tggcaaaagt atctgagaaa agcacttctt tccactgcgg cgctaaatag
    20461 accaaatgtt tggcctgtcc ggtgtattcc tgtgtaatct gaagctccat catcagcggc
    20521 gttttcggca ttgccccaaa cagcggatta aacggctctc gcggctgaaa gtcaatcggt
    20581 ccattcttcg tttgaatgaa gatattcgga ccgaattggc catccagcgg cacaaactct
    20641 ttataggcgc atttggctcg gtctgaatca atagaagtat cataaacgaa cgaacgccac
    20701 atcagaatgc ctccatgcgg ccgcaggacc tcggcgagca tattggcccc atccgcatgc
    20761 gtccggttgt acgcctgcgg gcccggctgg ccttccgaat aggccttgac cagaaatccg
    20821 ccaaaatcgg gaataaaacg atatatttcc tctgccttct gacgccacca ttgacgtacc
    20881 tgcggccgta gaggatctgc cgtatccagc cccccgattt caataggggc tgtaaatttt
    20941 acgctcaaat acacccgaat gccgtaagga cggaaaatat ccgccagcac agcggccttt
    21001 tgcaggtatt gacttgtcaa aacttcggct tgggcattta cattgttcaa cacagttcca
    21061 ttgatgccga tggaagcatt ggcacgggca taatccgtgt aacgctgttc aatctggctg
    21121 ggaagccggt tccactgcca caatgagcgg ccggcatagc cgcgctccac agaaccattg
    21181 agattatccc aatgattcag aagccgatac tgaatctgcg gcttctgatg aatgcagagc
    21241 tcttgaagcg gctgctgagt ctgcatcagc cggagcaggt gaaagacccc atagagtgct
    21301 cctatcggct ctttagcagc aacggctgtt atcggatgct tcttccagaa cacagagcaa
    21361 agaagatatc cttctgaacc aagactgtct attctgcttt tgctgaacaa ttcctgtata
    21421 atcggcgatg tctggtatgt gccgacgaca atggctccat cctgtgtaac agacgatgca
    21481 gaaggaaccg caactcccaa caaaccggac aggccctgct gcagctcctt ttcaatcacc
    21541 gtaaatgtct cggtctgccc ctgaataaca atttcagttg cctgcctgtg atactgcagc
    21601 agacaggcgg aatccgctct ttcataacgc atccacaagc ggtaaccgtc ttcgccggct
    21661 gccgtacttc cgagaaaaac agccagccaa tacagaatcc atcgttggct tctcatgcaa
    21721 cataaagtca tctgacacct gatcactttt atcataagca gagttgattt accggcttct
    21781 gtactgctga gataatttgt agatttcgct gccggccaaa agaaacccgc ctaatccata
    21841 atcttcaaaa ttgggcactt tgtcatacgt tacaggttgg ccgtcggagg gctgcttgcc
    21901 ggtactctga acatatccga gaaaaccgtt gggatgcagc gcttctttgg ccattccgtt
    21961 ccagccgcga acagcagccg gaagatagac ggcttcttcc agcatgcctt tgcgaacccc
    22021 ccaggcaaat ccataggtga agaatgccgt tcccgtcaat tccttgccgc cgaagttggt
    22081 tggatcgtgc agactgacat tccagaagcc gtccggccgc tgaatcggca gcagggcagc
    22141 ggccatttcc cggaaggttt tgatatactc gtcgcggtgc ggcgcatccg aaggcattat
    22201 gtccaaaacc cgaaccagcg ccgcaaacac ccagccgttg ccccgcgacc agtagcaatc
    22261 ctcgccgttg ggttcgcggt aaggcgggtc aaaatcctta tcccgccacc acagatgatc
    22321 ttgcggatta tacaggccgt tgtcgccgtg tttttccttg gtatatatat agagctgata
    22381 cattttttca aagtagcggt tatcctgata aagaactccc aaacgagcaa acaccggcat
    22441 tgccatctga agcgcatcaa tccaccacca gtcatcgttt ttatcactgc tgaccatggc
    22501 atcaatgcat tccttgattt gggcgatccg ctcgggtttg ggatcaatgc ggtataaatc
    22561 tatataggtt tgcccgcagc attggtcatc ggcattgcgg gttctgctgc cgctgcgcag
    22621 cccccattga tgggcttgtc cccaagcaac cgcatagtca taataggttt tctgcgggtc
    22681 gatttcataa agagccatca gcccttcata atacgtgccg cgggtccaga tattgctggg
    22741 acgcgtttta tccgttacaa tcggtttgcc ggggtccggc cacttatcca taaaatactg
    22801 attggccagc cgcatggctg ccagtatatc cgcttgcggg ggcagctcat agtcggcttt
    22861 agctgtccca gaatgaccag cctctttctg gtgttctgcc gaacagccag ctgtgctgaa
    22921 cataaaacct gccatcagag caattgccca tatccagtaa atcctgctgt ttttcattgt
    22981 gcatctccat tgggtatccg acttttattc ataaaatccg tattcataag ccgcatcata
    23041 cagcgcaacg atgttttcga caggaacttc ggcttggatg ttgtgaatat tgttaaagac
    23101 atagccgccg ccgggcttga atatttcaag atgccggcgg acttcttccc ggacctgctg
    23161 cggtgacgca aaaggcagta catgctggga gtcaatcgcc ccgccccaga aaaccagttt
    23221 atcgccaaac tggcgcttga tctgtgccgg ctccattcca gcagcaccga tttgaatggg
    23281 attgaggata tcaattccat tgtccagcag gtcaggaata tagagtttgc accccccgca
    23341 ggtatgatac cagattttgg ctgctgtcag caatttgata tgctgaatca gcttcttctg
    23401 acggggcttg acaacctttc gataaaactc cggattgaac agaggcccat cctgacctgc
    23461 caaatcatcg ccgagcatta caatatccac aaggtcgccg acttctctta aaaagccggt
    23521 ataataatcc atccaataag ccagcatctt atccagcagc atttcgcaaa aggctggatt
    23581 ttccatcata tctatatacc accgctccag cccccgcaga taccagcaag tctcataaat
    23641 caccccgccg atgccggtgc tgagggcata gggagtttcc tgacgcagac gagccgcttc
    23701 atcccgcaga ccggcaaaac gggacgggtc tttgccgttg ggaaacggat aatgtctcaa
    23761 ctccgtaatg gatgcatttg ccaaaggatg ataggaaata tccatataca gcggccggtc
    23821 atcgggcatt gaccatataa cgccgaattc atcggtcagg tcgtgccata tacggccgct
    23881 tcgccgattg gtttttatgg atccgtcaaa tcccgccgga cttctggatg ttatatagcg
    23941 aatatccaca tgaaaccgct gcaaaacagt ttcgctgggc ttggcaagct gctgaactgg
    24001 atccataatc tgaatcttct cttgaaggcc ccagtagtcc agcagattgt tgtaggcctt
    24061 tacatgaatg ccggtctgaa aaccgccgag atctatcggc acacggtccg gctgctgatg
    24121 gttcaatgca gcggataccc gctgacgcga agtcattgtc tctttcttca tttctgcact
    24181 ttcagaactg gatatccaac aagctgtttt actgcgggct gtactcgttt aaggcaataa
    24241 gggtcacagg ccccaaaaga cccgattcaa caatctccca ttgcgaagca tcaaactttt
    24301 tataatcaat attaacaata ttgatatccc caaagtattt ccacggcact tggcgtatat
    24361 ctaaatcccg aatgcgattg gccgaaagat ttgttacctc aatctccagc tgattggcat
    24421 tggacttcag cagtgtcttg ggtacttcga cggcaaacgg cacagagaac actgttccca
    24481 gagactgtcc gttcagacga acacgtgcac tggcagccac tctgcctaag tccagccgcc
    24541 aggcatcagc cctcatctgg ggcaagtcaa aagtcagacg atagcgggcc gtgcctgcaa
    24601 atcgctgagc ctcttggcct tgtttggtcc aagattcaag cacagtggtc gaaaaagaag
    24661 gcggaagctg cggcccgccc gtaataaaat ctactttcca gctgccttcc agcctcaggg
    24721 aatccgcatc ctgatgccat gtccatcgga cggcatcatt ttgtttttca ccaaaaagac
    24781 gcagaatcag cgattggccg gcttgcaatt ggatataaac ctgtgtcctt ccgtcctgtg
    24841 tctgccgcat tgggctgaat ccggttttgg catccattgg gtcgataata agaattgatt
    24901 gtgccggccg agtcagagta atccatccgg caaaagcaga cacagctgga ttttctgtaa
    24961 actgttcatc ctgattggtt acaaaatacc aatatccttt atcatctctg cggcggatat
    25021 aatataatcc cttatggtca accagagttt cgcgctgaat gccggcttga ttcattagag
    25081 atgcgacatc ttcactgaca taaaatgcac cttggccgac tttgcctgtc ttcaccaaac
    25141 cggactgcgg ctgccaaatt attgagtcca ttaatgcttt cagagcagcg cgacgtcttt
    25201 ccaagtcgcc caatcccggt acatcctgcg gcagcgcttt ctcaaacaga atgatcgccc
    25261 cctgctgagc cagcaaaaac aacttctgca gactgtcaag agggatatag gtacaatgcg
    25321 gcacaacaac aacccgataa tcgccgccgg gaactttgat tctgccggtg tcataataga
    25381 cctgctgaag ctggcggtca gaaatatagt caaaggcata tcctttgttc cataactctt
    25441 ttgccaatcg gccgatgggc tgctgggcca accattgcgt actgtggacg gtcatatcca
    25501 gattcatacc cttcggattg tgccagtagt cgctgattgg ccaatagacc agcaagtcat
    25561 tatccggccg gccggcctgc aataccgatt ggcatcgcgt aatataggca ttcagggcat
    25621 tcacatcata ccagatagaa ttgcggggat tcatctgggt ggatgcataa aagacccaac
    25681 cgggccattc ggcatctctg ggagaataac aggtaccgtg ataaaaaata tggttgattc
    25741 cgctgataaa cagcccatca ataaaatttt tcaaatggcc cagcgtcaca tgaaagtgct
    25801 cttttagcca tgttcctgtc tcgctgctgg ttcgcagacg gcctgccgta tgagccgccg
    25861 atgaagcgaa tttggccacc agcggattgc ggtccagtcg gaaaaactcc gtctcgggaa
    25921 tatcagcaag agcatacaaa tcaagaaggt ttcccggaga accgtgggct tcattgcggg
    25981 ttataaagcc gtttttatgc gaccattgga cccacagcga ataattttct accatcaaat
    26041 ctgacagcgt ttgacggtaa tctcccttca ggcggctagt ccgttcggtg tcttgactgt
    26101 atcccagaaa ctccggcaaa aactcttcga gacggtaacc ccgccgtttg taaaactcgc
    26161 tcagtaaatc cggcgaccaa ttggagacat attcatacga atcatgataa gtcgcccggg
    26221 gcttcagccc gctgtaatcc ttaaatgcct gatcgaacca agccagatat tcttccatcg
    26281 ccggccgata aaacggattg agcatatgcc cttcgccacc cggtgccgcc cgcttgacct
    26341 ttcggccgga cggcttttgc cagagttcat acacctgcca tgtaccgtcg gaagctgtcc
    26401 agtcgagggt gccatcgggt ttcaggcggc ttgtcaaatc caccgccttt tgtccgggac
    26461 cataagcagc aacatacaac ggcaatgcag atctgagttt taccgatgat tgacctgctt
    26521 caatagtctg cacggcatgc tcaacaaccg cgttggcctg ctgatttgta atcgtcggcc
    26581 ccccaaagca ccagccggtg cccagcgtca tatccacgcc cattccaagc tgacgggctt
    26641 gacgcactgt ataatccagc atctccaacc attgggggct gagatactca atatactgct
    26701 tttcgtatcc tttgacgccg tagatgggaa taatatggac tccgcccagc cctgcctttt
    26761 gatatgcctc caattgaaac gcaatactgt ccttctccac cgcgctgccc atccaccacc
    26821 aataggtcca cggccggcat tgggcctcca ttgccggcca cggcgaagcc gtcggttgtg
    26881 ctgcacagct gcagataaga aacatccatc ccataagccc tgttattaat aaccgcttgc
    26941 tcccgctgca ttttataaca gccacgtctt cagtcctttc ctgtcagcag tattagaagc
    27001 acctgtaagc acaatcacag gtaccttttc tccgaataag aataaccaat ttgcattaag
    27061 ggatcggccc ttcaaatatt tcttctgtcg ctgttttctg cttttgatag atgctcggct
    27121 gcgacccatc atctgccagc ttcggaccgg cataaagaac agccacatcg gctacagaag
    27181 cagtggaatc gcccccaaat ccagacaaga tatgtatttt aatgcggctg gcgtccgttg
    27241 ttttgccgaa atctatcgtt tgaggatgaa atgttgattt caattggcct tgtgctgcca
    27301 gtgtccaatt ctgtccgtca ctgctgattt caatcctgta atcacggaca tcaccttcat
    27361 gttcccggtg gttctggcgg ttcatccata caagcccctt cagcgcaacg gcctgcggaa
    27421 atgtaattgc caactcatac ggatgcccct gcccgcgtcc tcccgcaagc caataggtat
    27481 tgggattgcc gtcaaggata ttggaagccg gattgccggg attctgcatg ctggcggtaa
    27541 cagcggcctg cagtttcttc attattcggt tatcaaaatg cagcccttcc aactgctcta
    27601 acgaaatctc tgccgacgga ttaaagtgac tgccccccat ataatccagc agactccggc
    27661 gaagctggcg tgcggccggc cgcctatcga ggtttgactc caaatccgcc gcacacacca
    27721 gcagccggcc tgaaccgacg cggcattcaa atactgcggc cagtttataa ttgcggttcc
    27781 agtcatcaat catctgcaca atcggcttga gcgtcttggg cagagcatcg atattcacag
    27841 cccggcacgc agggctgaaa acatcctgcc attgccaatc gtaatgctca tccgtcggga
    27901 agaatgccag cgccggatga gacggctggc agaccagccc taaaaaccgc tcccagccgg
    27961 gacccatcag acgattccag aaaatcggca gccggccgat aggcggactt tgccactgaa
    28021 gctggtccga agtcggcatc agcaaaacct tctgcccttt ttgaagtgcc gctgccgccg
    28081 cttcgaaaga acgtgtaaga acaacatcct gcggcactgc tgtatccaat tgcgccggat
    28141 acagccagaa ctcccagtca ttctcaatct ccatgcccaa aagccccact acaagccggt
    28201 atttggccgg cgctttgaga ctgcccagag agattgaaac cgtccccagc ggaatagcgt
    28261 tttcgacagt tatggttttt ctttcaaact gaccgccggc agcttctttg ccgccggaat
    28321 caacaatttt ccaaaacggc tggacatcct gaagcggctg cgctccgaaa tgcgcaatct
    28381 cacaatcaac attgagctgt tcggctgttg tataaatctg gcgcttcatc cgcgccagcg
    28441 gaactgtcgc attgcagaaa cgccggaact ccgacggtgt aatgtatcct ttggattccc
    28501 agaaaacatt caaaatccca accagtgccg tcccttgtcc gggataatcg tgcagatcca
    28561 gaagctgaaa tccgcccatt ccgggtgttc gcagcaaggc ctcaatttcc tctttatagc
    28621 aggcggcctg cagcttcccg ctggcaagca gaaaatcgcc cgcttggtcc agcatgccgt
    28681 gttcggccag cgagtctcgg aatatctcaa aattcttcgg tttcagtttt ccggtgtatt
    28741 tggggatttc cgaaaaatcg ggatacacgc accattgacc tgtttcatgg gaaataacag
    28801 ggtacgtcgt tccttccaaa gaacgggaaa aatcgctgcc gaaccagccg gttgcaccgc
    28861 gaaacatata cgagccgatg cgcattgtat ccagatagtc aatatccggc acagggccgg
    28921 gacgttccgc aaagaaacgg ccagtctgac tggcatacag acgccggttg tcgcgggcct
    28981 tccagtcccg gcaccatttc agcaagggtt cctgccaagg cccggccggc tcattgccgg
    29041 ttgaaaacaa tacaaaagaa ggatgattgc cgtaagcccg gatgattcgc tctgtttccc
    29101 tttcgagcca ttccacaagg cgggtatcgc gggatgaata ctgtccccag ttggaacatt
    29161 ccggctgcag atagatgccc atttcatcgg ccgctgtaaa agccgcttcc ggaggacacc
    29221 aggaatgaaa ccgcatatgg ttaagaccat aatctttgca gatcttgaat attttccgcc
    29281 acgattccac atctgtgggc ggatagccgg tcagcggaaa ctgacaccct tcatgcgtcc
    29341 cgcggaagaa aacccggcgc ccatttaaat aaaagcgccc cccttcggcc ttgatttgcc
    29401 gcaatccgac tgtcagttcc cgctggtcgt cggcctgctg ccccgtcagc ttcagcgtca
    29461 gccggtgcag cgaaggatgg aactcatccc acagggcagc actgtccccc agcggataat
    29521 ccgcttctgc atatcctgcc tccgccgtcc attgtacggg aatcggctta tcattgacat
    29581 acaaggtacc gctgccggcc ttgccggtca tattgcctat ccatactttc aaatgtactg
    29641 tttttgtttc tatgtctgaa aacgcccgca catcatcaat ccaaacgggc gtcgtagagg
    29701 ttaaagaaag agtgccgatt aggccattcc agttggtttg cgtggaatcg gtgatgctgt
    29761 gagcgtcttc gcggatgtcc tgcagcattc ggttgtcaac acaaatcgtc aaccgatgcc
    29821 gccccggact tacaagacct aatgggtaaa tatgaggcgc tgaaagcccg tcattgaagc
    29881 cgatttgctt ctcatcgagc cagaccgtcg tagaccagtg gggtcgctcc agagtcagta
    29941 ccacgcgccg gccctgccag acggccggga tttctatgtc ccgctgatac cacgcccagc
    30001 caatataatg ccgttcgggc tgaagccaga agggtatctt gatattgccc ggctgggcat
    30061 atcgttcata gtctacccgc agataccaat gtttgtcatg cagccgcgac atccacggtg
    30121 tggctgtaga tacttcatct ccaaagccct gctcctgcag cgttccgggc aattggattg
    30181 tttccggcag cacccgctta aaccattcct gactgattcc ctgcccttct ttgtcaaggg
    30241 caaaccgcca cgttccgctt agagaaacag aggcagataa agatttcccc catccagccg
    30301 atacaatgct gattgcaaca aatacaagcc atccgtgctt catttgttca ctccagtatt
    30361 aaaataggat tagcaaaaag acaccagtca taaccgattc tgctgggacg gttttcttct
    30421 tctatctggc cttctgtagc agccagcgtc aaaaaccggt cgttttcatt taattcaata
    30481 gcaaccgtgt cgatacgccc tttggtctgc acatccgtct gcttgtaccg cacccgccca
    30541 tcgacaagca cccagaagtc cgccgagaag gagccgggaa cagattcgct gacgccgatg
    30601 tctgcctgaa aacaaacaat tcggacatcc ggcagacggg aacggaacgc atccaagtca
    30661 aaggttatcc ccagattggc atggagaaaa atacaggctt tgccgggctg gctgtaattg
    30721 atgccgtcca aaaccagcgg gtaacggcgg ccgtcttttt ctgtccggat aaattcaggg
    30781 caattggaga tatcagcgta atacaccccg gatgttgcag gacaatcctc aaagcaatgc
    30841 ccccgagagc tgacgacctg ctctgtttgt ccgttgggaa cgaatactcc gtcgataaaa
    30901 acactggacg gaattaaaat atacgtgttt gggcttttgc gaatcagatt ttccaccgtt
    30961 ccaaactgcc cgttttttgg attcaatccc attccgaagc ggccggttcc aaaaccgctg
    31021 ccgcccccca ccacatccga caggtcaatc ctatgccgtc ctctccaaag aaaacggctc
    31081 ttggaatcaa tcgcctgtac aaacgcctcc tgattcagcg ctatcggctt gacctcgctg
    31141 gtctgtcggt cgatgctgaa ggcctgccct gcagatacat caactgcatt tttagttttg
    31201 cttttcgtgc ttggaatcaa aacagttttc cctttgaaca catgcagctc ggttcctcgg
    31261 tgcggatctg cctgaacgcc gaaaagcgtc cccatgtcta taattttaga gtctgttgtc
    31321 tgaacactga aaccgtttcc catctcggat gttatcagcg tcagaagacg tccctgatcc
    31381 aatacaattt ctgagccgct tgtaaactga aatgccgcag gagcttccag aataatctga
    31441 actcctctgt ccgtctcaat tttgaccacg cctttatcga gctgaatcca atcggacatc
    31501 gctgaaatac gctggccttc acgaaatgct gctcccgaag cggattgtgc atctatcgca
    31561 tccagcactg ttgccacctc ataggacgga tacggatgga gataaatata aaccagcaaa
    31621 actaatattg ccgccaaaga aaccgctatc gtcagcagcg aggaccggga aaccctgcga
    31681 attggttttt ccaccggaat ttttgagaca ggtatttcgg attcatcctc ctgaggcaga
    31741 tggatggccc gtgcctgctt ttcatattca ccccagagct ggagcatctc gagagaaacc
    31801 gcctcggatg cggcctggag acactccggt tcttccaaac ggctctccag agacacagaa
    31861 atgcccttat tgtattgcgt ggccgcctgc agattgcaca gatccgtaca caaagacaca
    31921 taatcccaat aaagatccaa tacctgctgg ttttcggcca gcagtgcatc gagctcctta
    31981 aactcctgcc ggctgatcgt cccgtcccgc acacctccga acaggtaaaa caaacgattt
    32041 aattgttcgg gtgtaaaagt cacagcggtt ctccctccgc aagcatctgc cggatgcaca
    32101 gtagaagttt ggcgtggatt ttatttaaaa gccgatacag cacatgcagt tccttgccgg
    32161 tttgtaagga tacgcttttg gttgaagcac ccggctcata gcgcatctga atgagcgtct
    32221 gctcatggct tttcaattta ttcagacatt ttcgcaaaat ctccagacgc tcttcaacat
    32281 ccggcatctg ttttatcccc ttttcagcca gtgcttcaat aacttggtcg ctgaaataaa
    32341 gcttatcctt tttccttttt ttgtaataat tcagcacctg attatgtgca atttttaacg
    32401 cccatgcgga aaagttggtc ccccgctcaa actcaccaaa tttagaccac attacagagg
    32461 ccacttcctg aatcaggtca tccacatccg accaattagg tataaacgac ctgatgtatc
    32521 cgtatatgcg gcgctcattg gccagataca gttttataaa aagctgataa ttccttgtat
    32581 ttccgtgctt tgtctcttct cccattctgt ttccctctga gctcctaaac tggtttatta
    32641 tagccctcat tactggttag gatatagatg tttttataat tatttataaa aaaaatataa
    32701 aatctgtaaa aaagcggctt agaaactata tatccaataa agaaactgct cgaagggtaa
    32761 acttattgct cgggggtgta aatgagacta tgcgcgattg ctattattat atgcttgctg
    32821 ctgtcgcctt ctatggtata tgcggctcgc tatatggaaa atctcgaccg cggcacagtt
    32881 gctgttcgga taagctccaa tcaggtatat gtcgggtggc gcatgctcgg caccgaatgg
    32941 gggaaaaata tcggctatca tgtctatcgc ggctcaaccc gcattacctc aacaccaatt
    33001 accgattcga caaacattct tgatacaaca acactttcca gcacgtatag tatcagtgcc
    33061 gtcatagatg gggtcgaacg gccggcctct ccgccagttg cggtatggga tactttttat
    33121 cgggatattc ccctccagcg tccggcaggc ggcaccactc ccgatggagt cacttatact
    33181 tacagcccca acgactgcag tgtcggcgat ctcgatggcg acgggcagta tgaaatcatt
    33241 ctcaaatggg acccctccaa ttcaaaagac aactcgcaat cgggttatac cggcaatgtc
    33301 tatctggatg cctatgagct ggacggtacg ctgttatggc ggatcgattt gggcattaat
    33361 atccgcgccg gcgcgcatta tacgcaattt gttgtctatg atttagacag cgacggacgg
    33421 gcggaactgg tctgtaaaac agctgacgga acaaccgatg ggcagggaac cattctcggc
    33481 aatccgacag ccgattaccg caacagttcc ggctacattc tttccgggcc ggaatatctg
    33541 agtgtgttcg acggacagac cggtgccttc cttgacacag tcgattatat tcctccgcgg
    33601 ggcagagtca gcgactgggg cgattcctac ggcaaccgcg tggaccgctt tctggcctgt
    33661 gcggcctatc tggacggcgt gcatcccagt gtcgtgatgt gccggggcta ttacacacgg
    33721 gccgtcttgg ctgcttggga tttcagcggc ggcaaactga ctctgcggtg ggtctttgat
    33781 acgaataatg gttatgcctc ttacagggga cagggcaatc ataatctcag cgtagccgat
    33841 gtggatggcg acggctttga tgaaatcgtt tacggctcgt gcaccatcga tcatgatggg
    33901 acaggacttt acagcacagg cctcagtcac ggggatgcgc tgcatgtggg cgacctggac
    33961 cccagccgtc cgggactgga ggtttttgcc atccatgaac cggctggcga gactgtcatc
    34021 ggcacaagtt ttcgagatgc agccaccggc gaaatcatct ggagcatgac gccaggagat
    34081 gtggggcggg gctgcgcaga cgatatttat gcaggcaacc cgggggcgga aatgtggtcc
    34141 agcaacaaag gagggctgcg cgaccgctat ggcaattatc tgggccgttc gccaagttcg
    34201 accaactttg taatctggtg ggacagcgac cttatccgtg aactgctgga cagcaatcat
    34261 atcgataaat acggaaccag cagcgacaca cggctgctga cggcgaccaa ctgctcctcc
    34321 aacaacggca ctaaatcgac gccgtgttta agcgccgaca ttctgggcga ctggcgcgaa
    34381 gaggtcatct ggaggaccag cgataataca gctttgcgca tctatacgac aactgccctg
    34441 accagccatc ggctttacac cctaatgcac gacccgcagt accgtgcggc catcgcatgg
    34501 cagaatgtag cgtacaatca gccgccacac cccagttttc atatcggggc ggggatgagc
    34561 acaccgccga ttcccgatat tgtccctgca ggaattcctg ttttgtacgg cgatttcaac
    34621 aacgatgcca ttgttgattt tgaagacctg ccgtactttc tggatttatg gctgctaaac
    34681 agctgtacat tggccgcaga tgctgacttt aatcaggact gcatggttaa cctgtatgag
    34741 tttgctgctc tggcaggcaa ttgggcaggg tctgacgtga ccccgcccgc tgcaccggcc
    34801 ggcctgactg caacagcggg aaacgggacg gtatcgctgg actggagcga caacagcgaa
    34861 agtgatctgg ccggatacaa ggtctaccgg tcaatcatct caggctccaa ttacacgctt
    34921 gtatcacccc tgctgaacag ctccaattat atcgacaatg atgtcgtcaa cgggatgacc
    34981 tattactacg tggttactgc catcgataaa cagtcaaacg aatcccagat ttctgatgaa
    35041 gtattagcca aacccgatgt tataaccagc cttacaattc aggaaaatga gccgggtttt
    35101 ctcggaatcg tgacaggcac tattgattcc aatcacgccg gctttacagg aaccggcttt
    35161 gccaatacga acaatgctgt cggcagctat atcgagtggg ccgtcaacac ccctgcggcc
    35221 ggcagttatg acctccggtg gcgttttgcc aactttggaa caaaagaccg aacaggatca
    35281 gtcagcatca acggctccca gcaggccgcc ggcatcagtt tccccattac cggcgcttgg
    35341 acaacatgga atttgaccga agcggttaca gtctatctgg cggaaggaaa caatgtgatc
    35401 cgtctgacag cagaaacctc tgagggtctt gccaatattg actggattga gattacagga
    35461 ctttggttat ccgccggaaa aagctgaaaa aaagaggagt cgttatgaca taaatccttg
    35521 catctgaaaa aacagcaaaa caaacttttt tgtagatttc gtgtaaaaaa atcccgaaat
    35581 attgatatcc cttattggat aacaatggga ggatagtgcg gtacagtttt attttaataa
    35641 gagacaggaa tattcctcaa aatataacgt aacgggtaaa taacggaaat atacaacttt
    35701 taggaggtaa tttgtatgaa acgaaacatg taccttgcag tcctgttgtt cagtgtcctt
    35761 agtcttatgg cctatgcaga cgtaacaacg accgttacca gtgcggcgga taccggcatt
    35821 cgagatgcca ccaacaccta cggggcagat ccctatatgt atatctatgg ggcgccctac
    35881 catatgggct atgtacggtt taatctggcc cccctcagta tctacactgt actgaatgca
    35941 caattggtaa tgactgtgtc cggcggggcg ccacgcaacg actctctggt tacaggacgc
    36001 tgggcagtcc atggcctgaa taatgtttca ggcaatacac cgcaggactg gaacgaactg
    36061 accctcaacc gcagcaacac ggggcttgaa gtcgaatgga caggccaaac actcgacatc
    36121 gccggcggcc gtgtaacaaa tctggacgcc gatgacggcg ttggcacaac agagacgatt
    36181 gtaaacggtc cgggaggatc gtatgccgcc ggaacaacat taaccctcgt cgattccgca
    36241 accggcagca aaccgcttgt aaacttcctc cagagccgtg ttaatgacaa tggtctggtg
    36301 acttttatca ttcggcagga tgccggcggc agcagaggat atggccttgc caccaaagag
    36361 aatgcaacac agacctattg gccttacctg cagattacat atattcccgg cggcgcagtc
    36421 gaaccgaatc cggcagacgg cagcactatc acaaatctca atctctccca aatctgctgg
    36481 agaaactacc tcagcgacct tgctgttgtc tggttcggaa agggagatgc caatgagatc
    36541 gattacaaat cacgcttgac ttatctggcc actattgaag agccggccaa cgaaacctgc
    36601 ctttccatcc cggccgaaat ggtcccgctg gacgtaccgg ccacctatac ttgggttgtt
    36661 gagtcttgga aatatccggc caccgacccc aaccatattg gtgatccaaa tgagctgatg
    36721 aatatcaagg tgtggaagtt tcatacatca gctattcctg tcgtcaagac caatcccggc
    36781 gaacaataca aattccccgg cgaaacagcc gtcttctcgg caaccttcga agctctgacg
    36841 gaaatgaccg gagctatctg gcagcgaaac ggccagccgc tgaataccag cgatccggat
    36901 gttaacatca cgataaatgc ggcaggtgaa catttgtaca caacaacctt gactttaagc
    36961 aatgtttcag ccgcagatga tggggcttat acctgcattg ggcagaattc gggc
//
LOCUS       DAOTBO010000020        35119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_998_length_35119_cov_5.852669, whole genome shotgun sequence.
ACCESSION   DAOTBO010000020 DAOTBO010000000
VERSION     DAOTBO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 35119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35119
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_35119_cov_5.852669"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..2608)
                     /gene="rnr"
                     /locus_tag="PLE88_04205"
     CDS             complement(410..2608)
                     /gene="rnr"
                     /locus_tag="PLE88_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOM75704.1"
                     /translation="MELIKKQILTLLTRRDYKPMPPRMLAQLIGAAGEYSAFEAALEQ
                     MRRRQEIAIDAHNNNVSLPPMGHRVIGTFRANERGFGFIVPLVPNAYGDLYVSPENTG
                     GAMNEDTVVARVVKSGRRLGQVRYAGVIIDIVERGSSRFVGTLQRHQSFWYVQPEGRG
                     FYRPIIVDDVSASGAREGDKVVLEIVSYPTDSMPARGVIVQVLGRAGCYDAEIRSIMV
                     RYGLPEEFSAECRQQARLAAEGFRPENAEGRSDISDEVVITIDPPDARDFDDAISLKR
                     DRAGNWQLGVHIADVSRFIEQDSPLDQEARLRGNSVYLPQKVIPMLPETLSNGICSLQ
                     PNQKRFAKSVYITYDANGGILGAEFDNTIICSRCRLTYQEADAILHGRAAGYPGEVVA
                     LLKDMETLSRVIERRREKNGMLHLELPETELVYDDQGRVIDAHPADTSYPHTIIEMFM
                     VEANEAVARLLDRFGVPFMRRIHPEPDAYTTSSLGRFVKLCGLKVPRHLDRSAIQDLL
                     AAVKGSPMSYAVNMYVLRSMQKAEYSPLHIGHFALASSHYCHFTSPIRRYADLLVHRL
                     IDSYVKGKLTKIGRDEILDEAQLIELGKHLTFTEIQAEEAEKELQTVLILQMLSERIG
                     QDMEVVVSGLTNFGVFVQCTKFGIEGMIEPGDLGLDEWKYDDRSQAVVGKWSGESVHL
                     GQLMKVRIAAVNVAARQLFLTPAEPLVKERPGLNRRKFKRKTHSRQKRRQ"
     gene            complement(2700..2978)
                     /gene="rpsT"
                     /locus_tag="PLE88_04210"
     CDS             complement(2700..2978)
                     /gene="rpsT"
                     /locus_tag="PLE88_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOM75705.1"
                     /translation="MAHSLSAKKRVRQNAKRRAINRARKSMIKTQIKHLESALNAGDA
                     EKAKEQFRSLARRLDKVASTSTMHKNTAARIKSRMARKVNKLAAKTAG"
     gene            complement(3045..4445)
                     /locus_tag="PLE88_04215"
     CDS             complement(3045..4445)
                     /locus_tag="PLE88_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75706.1"
                     /translation="MDPDSYEANLEKARAFFERAEEVAATDNFDYAIDLYMEGLRLSP
                     DALEDGHAPLRRLALIRQGKGGKKPSVLEKMKHLMKGKTPLEDMLNAEYLLAKDPDNL
                     SYAESLLKAAVAGGYLRTAEWMAHIIFEANKASDKPSFDTYILLKNMYKQMQMYSNAV
                     VACTLALQLKPNDSILRDELRDLSASMTMEKGKYGKGQDFRDSIRNRDAQERLHRQDN
                     IVKDLDVKHQAVEQARRKIQQGQATVTNILELANALFELETPEGEEEALTLLDNAYAK
                     SKDFTFMKRHGEFRIKKLKNHIRQLTTATEKEPQNQALQKQLQQAMHQLDEVELDHYR
                     KCVDNYPTDLRMKYDYGRCLIKSQEFDRAIPLFQEAQKDPRLKAAAMDKMGLCFLLKG
                     WTEDAIEIFQKALDNCPNREGPLAKDITYNLARAYETSSNSEEALKLYRKLALIDFSF
                     KDVGQRIDNLRKNNIK"
     gene            complement(4618..7386)
                     /gene="polA"
                     /locus_tag="PLE88_04220"
     CDS             complement(4618..7386)
                     /gene="polA"
                     /locus_tag="PLE88_04220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOM75707.1"
                     /translation="MSKKLYIIDGHAHIYAAYYAPMRPLTAPSGEPTKAVFVFTTALL
                     GLIEKHKPDMLAVAMDSKTPSFRAKMYKEYKAHRPPMPEDLPGQINRIEQILEAMNIP
                     LLRVDGWEADDIIGTLAKQAAVRGIDAYICSKDKDMLQLLGEHIIAYDVKTDTVTDVK
                     TLQDEMGLTPLQIIDMMALQGDAADNIPGVPDVGPKTALEWIRQYGSLDNLYAHADEI
                     KGKRGENLRAYKQQAYLSRDLVIINCAAPVELNEQTFALRPPDKEKLAAIYRELGFTR
                     LLSSLEVPTEGGRTAQMESQSASGVQRDLFAQPEKQTAYKSGAKGHYELIDTVLKLEQ
                     FVSELQTQQRFAFDTETTHIDPMRADLVGLSFSWQAGSAYYLPVRAPMGQNRLDMADV
                     RKKIGPILADKKRYKIAQNLKYDYLVLENAGLPIALEPGRVFDTMVASYCLNADRESH
                     SMDAMAKDYLGIEPIPISALIGKGRNQLTFDRVDTAAACEYSAEDADVTYRLYEYLSQ
                     RLETVPALKDLFETLEMPLVEVLARMEANGVSLDTALLRRMSDEMMQTAEKLTHRIYA
                     LAGIPFNIDSTKQLAEILFDRLGLPSVRAGKTQRSTDADVLEELKNAHPIVPLIVEYR
                     QIVKLKNTYTDKLVQLINPRTNRVHASFNQTITATGRLSSSDPNLQNIPVRTELGRKI
                     RSAFIPAEKSGCILSADYSQIELRLLAHFSRDKQLLKAFAENQDIHRFIASQVYGVPL
                     NEVTSEMRNKAKAVNFGIIYGQGPFGLSKSIGISQAEAKQFIEDYYKRYPAIREFMNN
                     AINRAKTLGYAETILHRRRPISGLSSRNFNVRSQAERLTVNTITQGSAADLIKRAMIN
                     IHRRIERDKLPVKMILQIHDELVFELPRQGAEEHAAWIEKEMAEAIQLEVPLKVDVSI
                     GPSWLTEK"
     gene            complement(7389..7817)
                     /locus_tag="PLE88_04225"
     CDS             complement(7389..7817)
                     /locus_tag="PLE88_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75708.1"
                     /translation="MKPWTKTERIAVCIGCFMAVLSFFLLSAAIRSIRQANQMQLKYR
                     QIQPLVDRSVELSRQINAELWAVMEEAQAIVADQAKPADQQPSDLEQRPQLNRQRIEG
                     LAEIMTQQQRIIEQICEIQRQNGDSTRTESPETKAVLEKY"
     gene            complement(7881..8657)
                     /locus_tag="PLE88_04230"
     CDS             complement(7881..8657)
                     /locus_tag="PLE88_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOM75709.1"
                     /translation="MLQQIRNKKAFICDMDGVIYHGRRLLDGAAAFVDWLKREGKQYL
                     FLTNSSERSPAHLRYKLGKMGIEISEDHFMTSALATASFLHSQKAEARVYMIGGKGLE
                     EALREAGCILADEKPDYVVIGETREYSYDKLETAILAVQAGAKLIGTNPDISGPGEKG
                     IVPACGALTAPIELVTGKKAYFIGKPNPLIMRHSLKRLGCRREDTVIIGDRMDTDIIA
                     GIESEIETVLVLSGITSRQDMERFAYRPSLILDHVGQIAE"
     gene            9035..9331
                     /locus_tag="PLE88_04235"
     CDS             9035..9331
                     /locus_tag="PLE88_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOM75710.1"
                     /translation="MHIVHVFVDVKKDYIDKFKAATVENARHSRQEPGVIQFDVLQQQ
                     DEPSRFLLVEIYRSQQDATTHKGTAHYQKWRDTVADMMAQPRRSINYTMVSLPN"
     gene            9390..10346
                     /locus_tag="PLE88_04240"
     CDS             9390..10346
                     /locus_tag="PLE88_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOM75711.1"
                     /translation="MNVLIYGGGAVGLGIASCLIHSQIAVDIIARPQTVLQLRREGLF
                     RKGLFGDFFAAPTEFGSYSSLEALPTRIYDYILVCTKSYDTLQAAQDIRRHSGCFDAD
                     TRIVLFQNGWGNAEIFSSFFPKEQIYCARVITGFTRAKANEVVITVHADAIHIGRLFA
                     AASEKIQNLCSAITRGGIPCRPTNDIEKDLWAKMLYNCALNPLGAILNVPYGALAENK
                     STRFIMNSIIREIFAVLACTGYRTHWDDADSFLDVFYSRLVPDTAEHRSSTLQDMNAE
                     KKTEIDALNGAVIRLADTHHIQVPYNQTLYHIIKFREPLSGD"
     gene            complement(10504..11715)
                     /locus_tag="PLE88_04245"
     CDS             complement(10504..11715)
                     /locus_tag="PLE88_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOM75712.1"
                     /translation="MFDTTGKAQGNIKRKDSTLKKLQRMQKALHHEEPDRVPISDFFW
                     GSFIQRWRKELNLAGDANPYYYYDLDWIVTVPNMDPFIRPFEMLEESSKQVIVKTGFG
                     AVIRKKFDAPMPEFIAFETDTIDKLEALEFDDPWDRRRYFEAGDNQIAGVGDGFERNS
                     PPWIETVQSLRPDFPVFGSIIECNECLTRLIGPANNMLWIGMYPERMGRIIARIGEFY
                     FECAKAQIHAARGLLDGFVIWGDVAYKNSMFFSPDYWRRYFKPAVKAIADYAHEQGLM
                     VIYHGCGNINAILPDYIEIGIDALNPLEAKAGLDAVELRRQYGHKLGICGNSNIQIWE
                     SNDKEAIRKEVLRKLNAAKGGGYIFQSDHSVASNVSGHTYDYIVKLVRQYGNYPLHLG
                     QYNPSFVGDCI"
     gene            complement(11767..12843)
                     /locus_tag="PLE88_04250"
     CDS             complement(11767..12843)
                     /locus_tag="PLE88_04250"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOM75713.1"
                     /translation="MTISANPMLGIGFHSVGAMLAANCYAPQKLIRRWSWEIFWIVQA
                     SWCWLLWPIIGAVLTIPDLPNVLADAVRECPLALVWSFLFSMAYGVGGIAFNLSIRYI
                     GFSLTYAVAVGLSAVLGTVVPPLVRGQFGAILSKTGSGWVLFGVAIGALGIAVCGIAG
                     RLKEHDLAAQASGMGQFSLFKGFGLSLLAGILSAVYGFALDVAAPVAEIAESYGAGHW
                     KGNVIYLTANTGAFVTSMIYCLFLARKNASFRDFFRLKEGAESSSLWGNYILAILTGT
                     LWYGQFFFYNLGHVRMDQYAFTSWAIHMIMLVLFSNLTGILFHEWKACSFRTRLAIVA
                     GLAVLIVSVLLLTYGNYLGQQATY"
     gene            complement(12875..15148)
                     /locus_tag="PLE88_04255"
     CDS             complement(12875..15148)
                     /locus_tag="PLE88_04255"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOM75714.1"
                     /translation="MQAGLDLDAYNLIWNSPSLNAGQSMPCGGGNIGLNVWVENGDIL
                     FYVSQTGAYDENNCLMKFGRMRVKLSPNLLQEAAVFRQTLKLKEGYIEIVCKKGEHTA
                     TINLWVDVYKPVIHVDIACSQPISVQAIYESWRTTPRELADEENNSNRSFLGSGVKAL
                     VRNDTIAFRDDGILVYHCNDNNKPNAFDLCVEQQGLADVKDQMWNPLKNLISGGLICG
                     GNMEPAGTGEGRYASTDFKSWILKSIKPAVSHSIAIYLHIDHAETQQLWQQGLDRLVN
                     IYEKIDGREKTLAWWDEFWNRSYIAIQPSKESSNSLPWTIGRNYQLFRYQLGCNAYGE
                     YPTKFNGGLFTFDPEYVDQRITCTPDYRRWGGGSFTAQNQRLVYWPMLKSGDYDMMLP
                     QFDFYLRPLKNAELRTEVYWGHKGACFTEQMESFALPVGFEYGWKRPPYYDPGIEYNS
                     WVEYQWDTALEFCWMILEYYRYSGNDISRYIPLIESCLIFFDEHYQRLSSRRTPHSLD
                     EHGHLVLYPGTACETYKMATNSVTTVTALNTVITRFLELPAFYLDGQRRLYYKEYLKR
                     LPPISFRQKDGHKTIAPAERYERINNIELPQLYPVFPYGRYGIGRPDLEVAIDTWKYG
                     CDRPEQKGFISWHQDAIFCARLGLTDEAAEITAKKMRDSGRRCPTFWGPGHDWVPDHN
                     WGGCGMIGLQEMLMQAVDNKIYLLPAWPEDWDVDFKLHAPYQTTIEGKVRNGSLEELK
                     VVPQSRLKDIEICSKYK"
     gene            complement(15334..15672)
                     /locus_tag="PLE88_04260"
     CDS             complement(15334..15672)
                     /locus_tag="PLE88_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOM75715.1"
                     /translation="MKRYGSVIQVKEAKLDAYVKLHAAVWPEVLSMIKKCNIQNYSIY
                     LRKLPDGKYYLFSYFEYVGQDFDSDMKKMAADAATQKWWDVCKPCHEPFPDRAEGEWW
                     ASMEEVFHLD"
     gene            complement(16141..17727)
                     /locus_tag="PLE88_04265"
     CDS             complement(16141..17727)
                     /locus_tag="PLE88_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOM75716.1"
                     /translation="MQRHFLPIDWIVLAVYFLSTMCIGFYFYRKSRSREGFTAAGRAL
                     PGWVCGLSIFATYLSSISFLALPGKSYASNWNPFVFSLSLPIAAWIAVRFFMPYYRKT
                     GDVSAYSHLEHRFGPWARMYASSFYILTQLARIAVVMYLMALPMSILLDCDIRLIILI
                     TGISVTIYSFIGGIVAVIWTDAFQAVVLIGGAILCLLLILFNMPQGPQQVFQIAAEHH
                     KFSMGSVGGSLAQPTFWVVLVYGIVINLQNFGIDQGYIQRYIASKNHLEARKSIWLGG
                     LLYLPVSAVFFFIGTSLFAFYKVHPDYLEEVRLQVAQQQLIQEGFSRDQGEFSWELSN
                     RASLLIDQDLGDRVFPHFIGRMLPAGVTGLLVAAIFAAAMSTVSTSLNSSATLLATDW
                     YQRYIRPDASEKQFMTVLYAATIFCGLLGTGLSLLLINVTSALDAWWILSGILGGGML
                     GLFLLGLISRTVNSSIAAAAVVFGLLVIAWMTLSPKIKSWPVSLQSHFHAFLIPVFGT
                     LMILLTGLLLQFIIRPKQHL"
     gene            complement(17889..21134)
                     /locus_tag="PLE88_04270"
     CDS             complement(17889..21134)
                     /locus_tag="PLE88_04270"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOM75717.1"
                     /translation="MKRIMPLIVFNVGLVVCAGCLSSLNPASHQLIPVLLRCEYRTDP
                     IGIDIISPRFSWQLQSDQRGQKQTAYQIYVASDLQLLQNNQPDMWDSGKVQSDETAAV
                     AYAGKPLASDSVYFWKVRLWDKDGNSSPWSLPAKWSMGLLHQEEWQAEWIGYDIDEKP
                     IPTPDVVSRAKWVWAQPNANISAPPGTCYFRKAFQLPQDWIIQKAVCYYTADDFVQLH
                     LNGSLLRSFRNRKVIYEVVLADYLRPGVNVLAFRAVNEGNADSPAGFWAAVRIESADG
                     RILEFATDNEWLLSTDEQLGWKTVDYDDAGWSRAVEIGPLGITPWEQTKPMKRLLPPA
                     RYFRKDFAVSNKEIERATLYATALGIYQCSINGQRVTDDYLSPGWTDYRKRVYYRAYD
                     VTKQLRRGSNAIGGILADGWYAGYVGGGLNRNHYGRKTRMLLQLNIQYEDGTKQIVGT
                     AKDWKASTGPILYADLLQGEGYDARQELPGWDMPGFDESAWRPVIAGDTEVNPQIRAA
                     VTEPVRVFSSIKPVSVAEPVRGIYVFNMGQNFAGVVRLQVHGRPGQRIQVRHAERLNP
                     NGTIYTTNLRTASATDIYICKGSGKTEVWQPYFTQHGFQYVEITGLDEKPDLDTVTGL
                     VLTSDTPIAGSFDCSDPMVNRLYSNIVWTQRMNFIDIPTDCPQRDERFGWTGDAQVYI
                     NTACYNNDVQSFFTKWLMDLTDAQRDDGQFPRYAPLKVDPTDGGPAWADAGVICPWTI
                     YKMYGDKRILQTHYDAMKKFIEFNRKRCTSALLPPEKFQCFGDWLNINDETPHDVIYT
                     AYFAHSTHLFAMIADALGKTDDAQTYGTLFEQLKSAFSNAYVGPDGTIKGDTQTAYVL
                     AIAYDLLDPAHQKLAAEHLVRRIRECGYHLSTGFIGTKDLMLALAKIGRNDLAYRLLY
                     NDSFPSWGFTIKQGATSIWERWNGWTPQDGFADPGMNSFAHYSFGAVGQWIFENIGGI
                     QSQGPGFKTMMIHPQPDGKMMWAKTSYHSVRGLIVSNWEIRNGCFVLDVTIPPNTEAD
                     VFIPAQDAEKVLESGIPAQQAEGVTFLKQQAGTIVYKVKSGKYQFRSPWTP"
     gene            complement(21156..24488)
                     /locus_tag="PLE88_04275"
     CDS             complement(21156..24488)
                     /locus_tag="PLE88_04275"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOM75718.1"
                     /translation="MRLLSILLPASALWAAVGDEEEPVRFVGEVKVSNSDYKNGYHDG
                     QMPVAIGVQNYQILRANRTHPEFSDGLGWTYNHAPMLAYVNGQFYCQYLTNPCGEHIP
                     PGMTMLTRSLDGKHWTKPQVLFPIYFTADPNDATRITFVFMHQRMGFYLADNGRFLTM
                     AFYGSPDGDGVGRVVREIYADNSLGPIYFIRVNDNWTGEVKYPLYTASPDAGFVEACR
                     SFLEDKIRRIQWWEEDYQAKDRDDFYRVTWGGDIGRSRPAKAFCFYTRPDGAVVGFFK
                     SRWVTMTRDQGQTWSPLVQCSTLTYGSAKIWAQKLDNGQYALVYNPTDSEARHPLCIA
                     VSKDAIHYDHLVNIHSEVPPKRFWGREKRPGPQYVRGIVEGNGNPPGSDLWVVYSVNK
                     EDIWISRVPVPVQWQVQGSVADDFSQMDTGGVIKGWNIYSPQWCPIEVVDFPSKADKS
                     MRIKDADPYDYAKAVRVFQKSANLICSFQLYIESSAQVLDIEIVSTKGSRLVQTRLDT
                     SGRLLTKNGNQPYQTVAALNSGKWYSVEFHVDAPKQQYSVLLDGNMIVQDASFSSVEE
                     LPERIVFRTGPYRLADDVQKYKSGSERIPGTDEPGADEQVEAAVYYIQDFRAFDTGQS
                     VLSPRTFEHYIDDFNSMEEENIVNEIPNAQSWDWIKENVPLFECPDKTLEQIYYYRWW
                     TFRKHLKRTPDGYVFTEFLDSVGHSGKYNTISCAVGHHIYEGTWLRDCRYIDDYARFW
                     YTGHNGGLQPHFHKYSNWATAALYRRYLVNQDKLFIIGLLDAFVQDYQAWVDEKAAEN
                     GLFWQYDVRDGMEESISGSRTIKNMRPPLNCYMYANAMAIAKIAEVAGNLELSKEYIQ
                     KASDLKLRIQQLMWDGTSAFFKVRHPDGTLANVREELGYIPWYFNLPEKGFEAAWQQF
                     TDPQGFKAPMGITTAERRHPEFRSHGVGTCEWDGAVWPFATSQTLTALSNFLRYYSQS
                     YVSKKDYFEALLTYARSHQRNGKPYIGEYLDEISGIWLTPDSNRSRYYNHSTFCDLVI
                     SGLVGLLPREDDIVEVDPLLPDGAWDWFCLDRVVYHGKILTILWDRTGEKYNQGKGFR
                     IFADSKEIGHADELSHLTVKLQ"
     gene            complement(24565..26136)
                     /locus_tag="PLE88_04280"
     CDS             complement(24565..26136)
                     /locus_tag="PLE88_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5605 domain-containing protein"
                     /protein_id="HOM75719.1"
                     /translation="MNRFGCLVVLGIMYHCICFGADENVERWGVYELSLSGPAAGNPF
                     IGIQFTAVFKHGDQVFEPEGFYDGNGVFKIRFMPNSEGLWTYQTKSNCDALNGKTGQF
                     TCIAPSADNHGPVRVRNQYHFAYEDGTPFFPFGTTIYEWAFYSESMQTQTIETLKTAS
                     FNKARFLVIPPWQEYYVKGPHALNCFPFVGNSKDNWDFSRFNPEFFHRLERCVAELGK
                     IGVQAELILFRPYDNGKWGFDTMDMATNERFLRYVVARFAAYRHVWWSLANENSFMES
                     LTDQDWDHLFRILQRSDPYGHLRSIHNADRIYDHTQPWVTHVSLQYYMAVRFFGVSPL
                     LRDIYKKPVIHDEINYEGNRDRRWGQLSGEEMVYRFWIAYIGGAYATHGETIQRPNDP
                     DWISVGGDLKGQSPPRIAFLRKIAEEGPPEGLDPVDPYFETNIAGQIGNYYLIYFGKE
                     PLTEWEYKLPQKGLREGMRFRVDIIDTWNMTITPVNQEFEIEKQNQYTYIDKKKAKII
                     LPGKPYMALRIRRVD"
     gene            complement(26166..29303)
                     /locus_tag="PLE88_04285"
     CDS             complement(26166..29303)
                     /locus_tag="PLE88_04285"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOM75720.1"
                     /translation="MAQAELKKAGVFVLIALLVWTVAGYCADVPEISFLTLDKNGHLV
                     YGTDQKGNQIPDFSHCGYRGANHPIPDCTIRVVLSPVKGDNTGRIQAALDYVAMLPQD
                     SEGIRGAVLLRRGKYSVWGGLTINSSGVVLRGEGMGSDGTVLIAAGTNRRTLLRFAGI
                     NDRKWHSDRLRHVADEFVPVGAYHIRLDETKGLKAGDNICITRPSTQEWIEEMEMNRF
                     GGGLKGLFDWKPGSRNIVWDRVITDVNDLVITIDAPLTAAINPQATLQTYNWPGRICK
                     VGVENLCLRSEYNLDNPKDEDHAWMAVTVENAEDVWVRQVAMEHFAGSAVAVWESCRR
                     ITVEDCLSLAPISEEGGYRRHTFFTMGQQTLFLRCWSEKGRHDFSVGHCAAGPNAFVQ
                     CESFQSLEDSGPIESWACGVLYDNVRIDGNALRLGNRGSRGEGIGWAAANSVLWQCQA
                     AVIECDKPPTAWNWAFGCWGEFEGKGFWHESNSFINPHSLYVAQLQQRLGGKAQSISL
                     MKNETSSTTSPSVELAAEMTAHSEKKAVTLREYIEAAPKRQLIPTDSTDAKRIEDISS
                     IIPENHSNTSGKTIALSNGRLVCDNQLVIGGSTEVAWWRGNIRPDAAASFGVGITRFV
                     PGRNGPGYTDDLNDLTDRMIANGQAILEHNYGLWYDRRRDDHQHVRRMNGDVRPPFYE
                     QPFARSGVGTAWDGLSRYDLTKYNVWYWNRLKQFADLCDRKGLVLIHHNYFQHNILEA
                     AAHWADFPWRSANNINDTGFPEPPPYAGDKRIYMDEQFYDTRHPVRTSLHQAYIRQCL
                     GNFADNSNVIQMISQEYTGPVEFVQFWLDTIIDWQEKAGRSPLIGLSCTKDVQDQILS
                     DPKRSKVISLIDIRYWWYQGDGLLYAPQGGQHLAPRQHARLLHPRRTSFSQVVRAVRE
                     YRTRCSDKAVIYSADANYGWAVLMGGGSIPNLPSTTDKDLLKAVPSMNPYNLAGNPEG
                     QYALMDPAGDALIYADSGDTVVIDFSSIHADFTVSYINLKTGQSEQTQKHVSGGTIVQ
                     MELENTPCLLWLRRQ"
     gene            complement(29515..30588)
                     /locus_tag="PLE88_04290"
     CDS             complement(29515..30588)
                     /locus_tag="PLE88_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOM75721.1"
                     /translation="MCQQQPASFKPGLVLEEFVFTNAPFPSCHAATIAEVNGELVCAF
                     FGGTAERNPDVGIWISHTFSGKWTPVVEIANGTITPELRYPTWNPVLFLPKGAELLLF
                     YKVGPSPTQWWGMMMTSSDGGWNWSKPKTIIGEKGKALIGAVKNKPIQLSDGTILAPS
                     SREDSGWKVHIEASSDNGKTWTELTGPLNDPNEMSAIQPTLLTYPNGQIQMLCRSRQD
                     RILETWSGDGGKNWSPMQPTILPNPNSGIDGVTLADGRQLLVYNHSTRSQPAVGHKGR
                     GILNVALSRDGKNWQAALVLEYEDQPNSQFSYPSVIQTADGLVHIVYTWHRQRIKHVV
                     LNPEKLQTVPMPDGQWPKNAYPR"
     gene            complement(30853..31953)
                     /locus_tag="PLE88_04295"
     CDS             complement(30853..31953)
                     /locus_tag="PLE88_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75722.1"
                     /translation="MKLLWKKNWLQTQQHFTDWWKCKGFVLGCWGTGIASDGMPHEDV
                     ILPPPAADFQQYHTDPELVSARIHYEMAHKVWPADVLPVAWPDIGTVTLAPYLGAVPE
                     YKKDNIWYRPCIADPDTHPPLLYDPAHPYSRMLKAVLGRTVASSQGHYFVGMPAIVPN
                     LDVLAQLRGTEELLIDMIERPAWVHEKLEEIEIAYRQVFDSLYEIIKDEDGAMAFGYF
                     MLWGRGKTGLLQCDVAALFSPKMFQDFVVPYLRWSVSYLDYSMFHVDGHQCLGHIDAL
                     LEIEGLDAIEWTPDSQVPPAGSSQWYELYKRIRSAGKAVWVANLDADDVIPLLDAIGP
                     EGVYLNVISAGGQIIRPWQMEQLSDKLESYRR"
     gene            complement(31970..33958)
                     /locus_tag="PLE88_04300"
     CDS             complement(31970..33958)
                     /locus_tag="PLE88_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75723.1"
                     /translation="MNKRKETGLRIWMLAYTGAVLPFFFGCITTFQRPQTAFYISPNG
                     DDSAAGSLEQPWRTLQHARDFIRTINQNMHHDIVVYLRGGIYPLDRTFELTSLDSGMN
                     GYRIIYKAYQNEKPILNGAVPVRDWEHHVGNIWKARLVRDRKLRALYINGKRAMMAGM
                     SRGVIGQGGYGTFTITGKEEWASTSGTEFAGIKFKSDQVGVYRNPQNVELCQSNIWSE
                     PILCVKDMFYEDGYTIAMLQQPYGAIALNMSWVPFHADPRPDRPIYIRNAFELLDEPG
                     EFYFDDQADTVYYYSRGEDMTTAQVYAPAVEGLVSIKGENTSLRVRNIQFYGLTFAYD
                     DYLLSLLGDSRGWVSVQSLSQFVRYIENGNWHPNRYNDITIPKGTVTLCNADSIRLER
                     NRFEHLASAVALNLENDVINTAVVGNYFGDLMGTSVNIGHPQHYQIGDGQQFPRGIEG
                     VCKNILIANNYVHNACLDFRQLEGMTGFFVENVKIVHNEICGTPYGGIALGWWWGNAQ
                     IPPSTVAKNNSIMYNRIGSTHRVLQDGGIIYVLGEQPDSEIAYNYCYDGPRCIYPDDG
                     SAYWKIHHNVILNELSSSSENTVLWLHIWTKACHDMRIDNNYVRADNIRCEGTNCPIT
                     RTIVEPNAPPWSKQAEAIIKKAGLEPAYQDIKPGFKHD"
     gene            complement(33955..>35119)
                     /locus_tag="PLE88_04305"
     CDS             complement(33955..>35119)
                     /locus_tag="PLE88_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM75724.1"
                     /translation="LGWKLMLCIWLLAAVAASFTITGGLVAVMVTDSFQSILMILGAA
                     LLAVITASQLESFSALLHVQCGSTPSELTWKLLHPAGSENPWYAFVLGYPVLSLWFWC
                     SDQTIVQRALGAKNLKQSQGGTLFAAFLKIIPPFIFLLPGIFAAQLLPDIKDDKQVFL
                     SLVSTYLPAGLVGLIVSVLVAAVISTLDSGLNSFSTIFTLDIYKRWIAPTASEHQIKQ
                     IGRITTLFSTLLAVWIALFLSKAEGTNLFNLFQGIIGYMAPPVSAVFVLGIFWRRSTA
                     TAALLTLIVGSLISLVIGFCDITNIFANEAGEDIFPHFLLLSFYLFCSILVFMIAASL
                     LTQHSISETPLPTLAQTYRQNPGLGPVGLAGWILLAVIMVGLYLFFQYGMKAS"
BASE COUNT         9124 a         9205 c         7638 g         9152 t
ORIGIN      
        1 gcaattccca atggatcttc tgccagcagc cgccagctta taaataccct tccattttcc
       61 tgaggtatcg ctacaattcc acgccccagt gtctcaagct gccgcccgca tgcattaccg
      121 gcaaaaacgg ttatacctgc ggccagcaca gcctcaataa tatgcctcat agacccgcct
      181 ttttgaatat tatatcccgt actttatagc ccatataaaa gtcctgccaa aaatacatag
      241 tattgttaaa aacgtctgtc agattgactt tatggctata cacaatcgat taaatcactc
      301 tgatttggcc gactaattaa ggagatattt aattcaaaat atgcagtata ataaagcaaa
      361 agagctgctt acaggtgtaa tatcgatgaa tccacccaaa accggccatt tattgccggc
      421 gtttctggcg gctatgagtt tttcgcttaa atttgcggcg atttaggccg gggcgttctt
      481 tcaccagcgg ctctgccggc gttaaaaaca gctgccgcgc cgccacatta accgctgcta
      541 tacgtacctt catcaattgc cccaaatgca cgctttcacc cgaccacttg cctacaaccg
      601 cttgtgaacg atcatcatat ttccactcat ccagccccaa atcgcccggt tcaatcatcc
      661 cttctatgcc gaatttggtg cactgcacaa agactccgaa attcgtcaat cctgaaacta
      721 ctacctccat atcctgcccg attcgctcgg aaagcatctg caatatcaaa accgtttgaa
      781 gctctttttc tgcttcctcg gcttgaatct cagtaaatgt cagatgctta cccagttcga
      841 taagctgtgc ctcatccagt atctcatccc tgccgatttt tgtcagtttc cctttgacat
      901 aggaatcgat aagacgatgc accagaaggt cggcatatcg gcggataggg ctggtaaagt
      961 gacaataatg gcttgaagcc agcgcaaaat gcccgatatg caggggggaa tattccgctt
     1021 tctgcatgct gcgcagtaca tacatattga ctgcatagga catggggctg cccttgacgg
     1081 ccgccaataa gtcctgtatc gccgaccggt ccagatgccg gggcactttc agtccgcaca
     1141 gtttcacaaa acgtcccaaa ctggatgttg tataggcatc gggttctgga tgaatgcgcc
     1201 gcataaacgg tacaccgaaa cgatccagca gccgggcaac cgcctcgttg gcctctacca
     1261 taaacatctc gataatcgta tgcggatagc tggtatcagc cggatgtgca tcgataaccc
     1321 gtccttggtc atcatacacc agttccgtct caggaagttc caaatgaagc attccgtttt
     1381 tctctcgccg gcgttctatc acacgcgaca gtgtctccat atctttcagc agagccacaa
     1441 cttcgccggg ataaccagcg gctcgtccat ggagaatcgc atcggcttcc tgataggtca
     1501 gccggcatcg agaacaaata attgtgttgt caaattcagc ccccaaaatg ccgccgtttg
     1561 catcataggt gatatagacg cttttggcaa accgcttttg gttaggctgg agagaacaga
     1621 tgccgttgct gagcgtctcc ggaagcatcg gaataacctt ttgcggcaaa tagacactgt
     1681 tgccccggag gcgggcttcc tgatccagcg gcgagtcctg ttcaataaag cggcttacat
     1741 cggcaatgtg caccccaagc tgccagttgc cggcccgatc ccgttttaag ctgatagcat
     1801 catcaaaatc acgggcatca gggggatcaa ttgtaataac cacttcatca ctgatatcgc
     1861 tgcgtccttc ggcattttca ggacgaaatc cttcggcagc cagccgcgcc tgctgacggc
     1921 attcggcgga aaactcctcc ggcagtccat aacggaccat aatagaacgg atttccgcat
     1981 catagcagcc tgcccggcca agaacctgca cgataacacc gcgtgccggc attgaatctg
     2041 tcggataact gacaatttcc agtactactt tgtcgccttc tcttgcaccg ctggccgaaa
     2101 catcatccac aataatcggc cgataaaagc ctcttccttc cggctggaca taccagaaac
     2161 tctgatgacg ctgaagtgtc cctacaaacc ggctgctgcc gcgttcaact atatcaatta
     2221 tcacacctgc atagcgcact tgtcctaagc ggcgaccgct tttgacaaca cgtgcaacaa
     2281 cggtgtcttc attcatcgcc ccgccggtat tttcgggact gacgtacaaa tccccatacg
     2341 cattcggcac cagcgggaca ataaatccaa aaccgcgttc attagcccga aaagtgccga
     2401 ttacgcgatg tcccatcggc ggaagtgaaa cattattgtt atgtgcatcg atggcaattt
     2461 cctgcctgcg gcgcatttgt tccaatgccg cctcaaatgc agaatattcg cctgctgccc
     2521 ctattaattg ggcaagcatg cggggcggca ttggtttgta atcccggcgt gttaagagag
     2581 tgagtatctg ttttttaata agttccataa ttgttcaaaa aggcacaaaa aaggcgtcca
     2641 gccgatactt atatcggccg gacgccttac tatctaaaac aaaacttgcc ttgtccaaat
     2701 tagccagcgg ttttagccgc cagtttatta acttttcgag ccattcgcga tttgatacgg
     2761 gctgccgtgt ttttatgcat cgtactcgtg gaagcaacct tatccagacg ccgtgccagc
     2821 gaccgaaact gttctttggc tttttctgca tcgccagcat tcaatgccga ttcgagatgc
     2881 tttatttgag tcttaatcat acttttgcgg gcccgattta tcgcccgacg cttggcattc
     2941 tgacgaactc tttttttggc tgataatgaa tgtgccactg tttactccta aatttccgat
     3001 tttaatagtc tgaaataaaa ttatctgatt tcaaaagaaa acaactattt tatattattt
     3061 tttcttaaat tgtcaatacg ctgccctaca tctttaaaac taaaatcaat tagggctaat
     3121 ttccggtaga gtttcaacgc ctcttcagaa ttactactcg tttcataagc ccttgccaga
     3181 ttgtaagtta tgtcttttgc caaggggcct tcgcgattcg gacagttgtc cagcgctttt
     3241 tgaaaaattt ctatagcatc ctctgtccat ccttttaaca gaaaacagag ccccatttta
     3301 tccatcgccg ctgccttcaa acgcgggtcc ttttgggcct cctgaaacag cgggattgcc
     3361 cggtcaaatt cctgactttt aataaggcag cggccgtaat catatttcat ccgaagatcc
     3421 gtcggataat tatctacaca tttgcggtaa tgatccagtt caacctcatc cagctggtgc
     3481 attgcctgct gcagctgttt ctgcagtgcc tgattttgcg gttccttttc agttgctgtc
     3541 gtaagctgac ggatatggtt tttcagcttt ttaatgcgaa actccccatg ccgcttcata
     3601 aaagtaaaat ctttggattt ggcgtacgca ttatccagca gtgtaagggc ttcctcctcg
     3661 ccctcggggg tttcaagctc aaacaacgca ttagccagct caagaatatt agttactgtt
     3721 gcctgtccct gctgaatttt ccggcgggct tgttcaaccg cctgatgctt gacatcaagg
     3781 tctttaacaa tattatcctg acggtgaaga cgttcctgtg catcccggtt gcgaatagaa
     3841 tcccgaaaat cttgcccttt gccgtacttg cccttctcca tcgtcatact ggcgcttaag
     3901 tcgcgcagtt cgtcccgcag aatactatcg ttgggcttca gctgaagcgc cagtgtacag
     3961 gccaccaccg cattggagta catctgcatc tgtttataca tatttttcag cagaatataa
     4021 gtatcgaaac tgggtttgtc gcttgcctta ttggcctcaa aaataatatg ggccatccat
     4081 tcggccgtgc gcaaataccc ccctgcaacg gctgccttca gcagagattc tgcatatgac
     4141 aaattatcag ggtctttggc cagcagatat tcggcattga gcatatcctc cagcggtgtt
     4201 ttgcccttca tcaaatgctt cattttttcc agaaccgaag gttttttccc tccttttccc
     4261 tgacgtatca acgcaagccg ccgaagcggg gcatggccat cctccaaggc atccggggat
     4321 aagcgaagac cttccatgta caggtcaatt gcataatcaa aattgtctgt agcagccact
     4381 tcttcagccc gctcaaaaaa cgcacgggcc ttttccaagt tggcttcata agaatctgga
     4441 tccatatttt atactcttta tcgttaagct tgcggcctat atcatactgt ttcagaccga
     4501 tttatgcaac aattttattt acgacgattt cttttgcccc gatacatttt taatacccaa
     4561 gctgcaaaca gtaacccttt ttctcctgtc aatctgccag cccgcctgaa atcccttcta
     4621 tttctctgtc agccaactgg ggccgatgga tacatccacc ttcagcggca cttccagttg
     4681 aattgcctcg gccatttctt tttctatcca ggcggcatgt tcctccgctc cctgccgggg
     4741 caattcaaaa accagctcat catggatttg aagtatcatc ttgacaggca gcttgtctct
     4801 ttctatccgg cggtgtatat taatcattgc ccttttaatt aaatccgccg ctgatccttg
     4861 ggttatcgta ttcaccgtca gccgttctgc ctgtgaacgt acattaaaat tcctgctgga
     4921 cagtccgctg ataggacgcc ggcgatggag gattgtctct gcataaccga gagttttagc
     4981 cctgttaatc gcattattca taaactcgcg aatcgcagga taccgcttgt aataatcttc
     5041 aatgaactgt ttggcttcgg cttgactgat cccgatggat ttagacaatc cgaaggggcc
     5101 ttgcccataa atgattccga aattcaccgc ctttgcctta ttgcgcattt cgctggtcac
     5161 ttcattcagc ggcaccccat aaacctgcga agcaataaag cgatgaatat cctgattttc
     5221 cgcaaatgcc ttcaaaagct gcttatcgcg tgaaaaatgc gccagcagcc gcagttctat
     5281 ctgcgaatag tcggcgctca gaatgcagcc gctcttttca gccggaataa atgctgaacg
     5341 tatcttccgt cccagttccg ttcgcaccgg aatattctgc aggttcggat ccgacgaaga
     5401 cagccgcccc gttgccgtaa tggtttgatt gaacgaagca tgcacccgat ttgtacgcgg
     5461 attaatcaac tggaccagct tgtcagtata agtattcttc agcttgacaa tttgacggta
     5521 ttccactatc agcggcacaa tcgggtgtgc atttttcaat tcttccaaaa catcggcatc
     5581 cgtactccgc tgggtcttac ccgctcgcac gctgggcagt ccaagccggt caaacaggat
     5641 ctcggcaagc tgcttggtgg aatcgatatt aaacggtatg ccggccagtg cgtaaatccg
     5701 atgggtaagt ttttccgcag tttgcatcat ctcatcggac attcttcgca gcagcgccgt
     5761 atccagcgac acaccattgg cctccattcg cgccagcacc tccaccagag gcatttcaag
     5821 cgtctcaaat aaatccttca atgccggcac ggtctccagc cgctgggaca gatactcata
     5881 cagccgatag gttacatccg catcctctgc agagtactcg catgccgctg cggtatcaac
     5941 cctatcaaat gtcagctgat tccgcccctt gccgattaaa gccgaaatgg gtatcggttc
     6001 gatgcccaga taatccttgg ccatcgcatc catactatga ctctcgcggt cagcattaag
     6061 gcagtacgag gccaccattg tatcaaatac acgccccggc tccagcgcta taggcaatcc
     6121 ggcgttttcc agtaccagat aatcatattt gagattttgg gcgattttat accgcttttt
     6181 atcagctaaa atcggtccta tctttttgcg aacatcagcc atatcaagcc ggttctgtcc
     6241 cattggcgcc ctaaccggca gataataggc gctgccggcc tgccaagaga aacttaatcc
     6301 caccaaatca gcccgcatcg ggtcgatatg ggtagtttcg gtatcaaatg caaaccgctg
     6361 ctgtgtttgg agttcgctga cgaattgttc cagcttcagg accgtatcaa tcagctcata
     6421 atgacccttg gcaccagact tatacgctgt ctgtttttcc ggttgggcaa acaggtctct
     6481 ctgaacgcca gaggcagatt gggactccat ttgagcagtc ctgccgcctt cggtgggcac
     6541 ttccagcgat gaaagcagcc gcgtaaaacc caattctcta taaatcgctg ccagcttttc
     6601 cttatccggc ggtcgaagtg caaaggtttg ttcattcagt tccacagggg cggcacagtt
     6661 aataattact aaatcccggc tcaaatacgc ctgctgttta tatgcccgca gattctcgcc
     6721 ccgcttgcct ttaatttcat cggcatgggc atacagatta tccagcgaac catactgccg
     6781 tatccattcc agcgccgttt tcggcccgac atccggcaca cccggaatat tatccgccgc
     6841 atcgccctgc agagccatca tatcaataat ctgcagcggt gtcagtccca tttcgtcctg
     6901 cagggttttt acatctgtta cggtatcggt ttttacatca taggcaatta tatgttcacc
     6961 cagaagctga agcatatctt tgtctttgga gcagatatac gcatcaatcc ctctgacggc
     7021 ggcctgcttt gccaacgtgc caataatatc gtcggcctcc cagccgtcta cccgcagcaa
     7081 cggaatattc atcgcctcta aaatctgttc aatacggttg atctggccgg gcaggtcttc
     7141 gggcataggc ggccgatgag ctttgtattc tttatacatc ttggcccgaa aactgggcgt
     7201 cttggaatcc atcgccactg cgagcatatc cggcttgtgc ttttcaatca gtcctaaaag
     7261 ggctgtcgtg aaaacaaata ccgccttggt cggttctccg ctgggcgctg tcagcggccg
     7321 catcggcgca taatacgctg cataaatatg cgcgtggccg tcaataatgt acagcttttt
     7381 tgacatattc agtatttctc caatacagcc ttggtttcag ggctttcggt ccgggtagaa
     7441 tccccgtttt gtctctggat ttcacaaatc tgctcaataa tgcgctgctg ctgtgtcata
     7501 atttctgcaa gtccttctat tcgctgccgg ttgagctgtg ggcgctgttc gagatcagac
     7561 ggctgctggt cagctggctt tgcttggtca gcaacaatag cttgggcctc ttccatcacc
     7621 gcccatagtt cagcattaat ctgccggctc aattctacgc ttctgtccac cagcggctgg
     7681 atctgcctgt acttgagctg catttgattt gcctgccgga tgcttcgaat cgctgcagac
     7741 aacaaaaaga aagaaagtac cgccataaaa cagccgatgc ataccgctat tcgttctgtt
     7801 tttgtccatg gcttcataag aatacctatg ttttacatat acttagacaa aatcaactat
     7861 cgaccgattt tgtatgtctt ctattctgca atttgcccca catggtcaag aatcaagctt
     7921 ggccgatagg caaaccgttc catgtcctgt ctgctggtaa tgccgcttaa aaccagcacc
     7981 gtttcaatct cagattctat ccccgcaata atatctgtat ccatccgatc tcctataatc
     8041 acggtgtcct cccgccggca tcccagccgt ttgagagaat gccgcataat caggggattg
     8101 ggtttcccga taaaatacgc ctttttgcca gtaaccagtt caatcggtgc tgtcaatgcc
     8161 ccgcaggccg gcacaattcc tttttcaccg ggtcccgata tatccggatt ggtgccgatc
     8221 agtttagccc cagcctgcac cgccaaaatc gccgtttcca gtttatcata ggagtattcc
     8281 cgggtttctc ctataactac atagtccggc ttttcatcgg ccaaaatgca gcccgcctca
     8341 cgcagggctt cttctaatcc cttgccgcca atcatataaa cacgggcctc ggctttctgg
     8401 gaatgcagaa aactggctgt ggctaaagca ctggtcataa agtgatcttc cgagatttca
     8461 atacccattt tccccagttt atatcgcaaa tgagctggtg aacgctccga actattggtc
     8521 agaaacagat actgcttgcc ctcccgttta agccaatcca caaaggccgc cgcaccatcc
     8581 agaagccgcc ggccgtgata aataactcca tccatatcgc agataaacgc ctttttgttc
     8641 cgaatctgct gtagcatttg ttttccccat ttacagtttt acatccagcg ataaaattat
     8701 actataatgc cggatgcttc tgaagtgaac gaaaaaggcg ggtaattcga tgattgagga
     8761 gttaaattat ggaaaatctg cttccacttc aaactttccg ccttatctgc ttgattccca
     8821 attctcaaaa gaaaagaccg tattatgggc tatggaacat ttttatcgga tacctaattc
     8881 ctattcgaca catattcggc atagccatta tccagaatat agacaaatgg atttgattga
     8941 ggtatcttct tcgcagctgc aggggagatt tttgctcttt tttcaggtat tgtgaaattt
     9001 aaatcttaca ggcaatcgta aaaaggattc aagaatgcat attgttcatg tctttgtcga
     9061 tgtcaaaaag gattacatag acaaatttaa ggctgccact gttgaaaatg cccgtcacag
     9121 tcgtcaagaa ccgggagtta tacagttcga cgtgctccaa cagcaggacg aacccagccg
     9181 ctttctgctg gtcgagattt accgctcgca acaggacgct acaactcata aagggaccgc
     9241 acattaccaa aaatggcgcg acaccgtcgc cgatatgatg gcccagcctc gcaggagtat
     9301 taactataca atggtaagcc ttcccaacta aaaaacggcg ggacattgtt ttgaaattat
     9361 tgcgttttta tcggagccaa aaacagagga tgaatgtact gatttacggc ggaggtgcag
     9421 taggattagg tattgcaagc tgtctgattc actcccagat tgccgttgac attattgccc
     9481 gaccccagac ggtgctgcag ctgcgcagag aaggattgtt tagaaaagga cttttcggag
     9541 actttttcgc tgccccgacg gagtttggct catacagctc gctggaggca ttgcctacac
     9601 gtatttatga ttacatactg gtctgcacaa aatcctacga taccctgcag gcggctcaag
     9661 atatccgcag gcattccggc tgttttgacg ccgatacgcg gattgtgctc tttcaaaacg
     9721 gctggggcaa tgcagagatt ttctcttcat ttttcccgaa agagcagatt tactgcgccc
     9781 gcgtcataac cggttttacc agagccaaag ccaatgaagt ggtcatcact gtgcatgccg
     9841 atgcaattca tataggaaga ttgtttgcgg ccgcatccga aaaaatacaa aatctctgct
     9901 cggctattac acgtggcggc atcccctgcc ggccaaccaa tgatatcgaa aaagatctat
     9961 gggctaaaat gctctacaac tgtgccctta atcctcttgg agctattctc aatgtacctt
    10021 atggagcgct ggcagaaaat aaatcaaccc gctttatcat gaacagcatc atccgcgaga
    10081 tatttgcagt gctggcctgc acaggatacc gcacgcactg ggacgatgca gacagttttc
    10141 tggatgtctt ctacagtcgg cttgtgcccg acaccgccga gcatcgttca tcgaccctgc
    10201 aggatatgaa cgcggaaaag aaaaccgaaa ttgatgctct caacggggct gtcatacggc
    10261 tagctgacac ccatcatatc caagtcccct ataaccagac actctatcat attatcaaat
    10321 tcagggagcc gctgtcaggg gattaaatat agatactggg cattggcgat ttcggttcgg
    10381 cttacataaa acgggttaac cgcagcagtg tatcggaaaa tatcactaat agtcgtttta
    10441 ccgcctttga taattcggct gattgggcga cccaaacagg aggttttacc gcaaagcagc
    10501 ggattaaata cagtcgccga caaatgacgg attatactgg cctaaatgaa gcggatagtt
    10561 tccgtattgg cgaaccaact ttacaatata atcataggta tggccggata cattgctggc
    10621 gaccgaatga tcagactgga aaatatagcc gccgcctttg gctgcattca gcttgcgaag
    10681 gacttccttg cgaattgctt ctttgtcatt cgactcccaa atttgaatat tactgtttcc
    10741 gcagatgccg agtttgtgtc cgtattgtct ccgaagctcc acagcatcca atccggcctt
    10801 ggcctccagc gggttcaggg catcgatgcc tatttcgata taatcgggca gaattgcatt
    10861 gatattgccg cagccgtgat aaatcaccat cagtccttgt tcatgggcat agtcggcaat
    10921 cgctttgaca gccggcttga aataccgtcg ccagtaatcg ggcgaaaaaa acatgctgtt
    10981 tttgtatgcc acatctcccc agatgacaaa gccgtccaaa agtcctcttg cggcgtgaat
    11041 ctgtgctttt gcacactcga aataaaactc gccgatgcgg gcaattatcc tgcccattcg
    11101 ttcggggtac ataccaatcc agagcatatt attggccggg ccgatcagcc gggtcagaca
    11161 ttcgttgcat tcgatgatgc tgccgaagac aggaaaatcc ggccgtagcg attgtaccgt
    11221 ctcaatccag ggcggggaat ttcgctcaaa accatccccc actccggcaa tctggttgtc
    11281 gcccgcttca aaatagcggc ggcggtccca agggtcatca aactccaggg cttcaagctt
    11341 atcaatcgta tctgtctcaa aagcaataaa ttcgggcatg ggtgcatcga actttttgcg
    11401 aatgacggcg ccgaaaccgg ttttgacaat aacctgttta ctgctttctt caagcatttc
    11461 aaacggccga atgaatgggt ccatattcgg aacagtaaca atccagtcca agtcgtaata
    11521 atagtacgga tttgcatcgc cggcaaggtt cagctcctta cgccagcgct gaatgaaact
    11581 gccccagaaa aaatcgctta tcggcacacg gtctggttct tcgtgatgca gtgctttctg
    11641 catccgttgg agttttttca gcgtgctgtc tttgcgttta atattcccct gcgcttttcc
    11701 ggttgtatca aacataccca tgtgtctgcc tctggatgaa tagattccgt gttattctaa
    11761 acttattcaa taagtcgcct gctggcccag atagttgccg taagtcaaca gcaaaaccga
    11821 gacaatcagc acggccaatc ctgctacaat tgccaggcgc gtgcggaagg aacaggcctt
    11881 ccattcatga aagagaatgc cggtcaagtt gctgaacaaa accagcataa tcatatgaat
    11941 cgcccagctt gtaaatgcat actggtccat gcgcacatgg ccgagattat aaaagaaaaa
    12001 ctgcccatac cacagggtcc cggtcagtat tgccaagata taattacccc ataagctgct
    12061 ggattcagca ccttctttga ggcggaaaaa atctctgaat gaggcatttt tccttgccag
    12121 gaacaagcaa taaatcatcg acgtcacaaa ggcgccggtg ttggctgtca gataaatcac
    12181 attgcctttc cagtgtccgg cgccgtaaga ctcagcaatc tcggcaacag gcgcagctac
    12241 atcgagggca aatccgtaca ccgccgataa tataccagca agcagcgaca gtccaaatcc
    12301 cttgaacaga ctgaactggc ccattcctga agcctgtgca gcaaggtcat gctctttgag
    12361 ccggccggca atgccgcaga cggctatacc caatgcgcca attgccacgc cgaaaagaac
    12421 ccagcccgaa ccggtttttg acaggatagc cccaaactgc ccgcgcacca gaggcggcac
    12481 aaccgtgccc aataccgcag atagcccgac ggcgacagca taagtcagcg aaaatccgat
    12541 gtagcgaatg gacagattaa atgcgatgcc tccgacacca taggccatac tgaataaaaa
    12601 cgaccagacc aaggccagcg gacattctcg cactgcatct gccagcacat tgggcagatc
    12661 gggaatcgtc agcaccgcac caatgatcgg ccacaacagc cagcaccacg atgcctgcac
    12721 gatccagaat atttcccatg accagcggcg gatgagtttc tgcggcgcat agcaatttgc
    12781 cgcaagcatt gcgccgacac tgtggaaacc gattcccagc atcggattag cagaaattgt
    12841 catcgtttta tttctctctg gaagttatca aatatcactt atatttgctg caaatttcta
    12901 tgtctttcaa tcggctttga ggcactactt tcagttcttc caaactgccg tttctaactt
    12961 ttccctcaat tgttgtttgg tacggcgcgt gaagcttaaa atcgacatcc cagtcttccg
    13021 gccatgccgg caaaagataa atcttattat ccactgcctg cataagcatt tcctgcaaac
    13081 cgatcatgcc acaaccgccc cagttgtggt cgggcaccca gtcatgcccc ggcccccaga
    13141 atgtcggaca tcgcctgcca gaatcccgca tcttttttgc ggttatctca gccgcttcgt
    13201 ccgtcagccc aagccgtgca caaaaaatgg catcctgatg ccaactgata aatcctttct
    13261 gctccggccg gtcgcatcca tatttccagg tatcaatggc aacttccaga tccggcctgc
    13321 caataccata cctcccgtag gggaaaactg gataaagctg cggcagttcg atgttgttga
    13381 tgcgttcata tcgttcagcc ggggcaatcg tcttgtgtcc atctttctgg cgaaagctga
    13441 tggggggaag ccgtttgagg tattccttat agtataggcg tctttgccca tcaagataga
    13501 aagccggcag ttccagaaat cgggtaatta ccgtatttaa tgcagtcact gttgttacag
    13561 agttcgttgc cattttgtaa gtttcgcatg cggtcccggg atacagcacc agatgcccat
    13621 gctcatccag agagtggggt gtcctgcgcg aagacagcct ttgatagtgt tcatcgaaga
    13681 agattaagca gctttcaatc aatgggatgt atcggctgat atcattgccg gaatagcggt
    13741 agtattcaag aatcatccag cagaattcca gtgcagtatc ccactggtac tccacccatg
    13801 aattatactc aatcccggga tcataatagg gaggccgctt ccagccgtat tcaaatccta
    13861 ccggcaaagc gaaactttcc atctgctcag taaaacatgc tcccttatgc ccccaatata
    13921 cctccgtgcg cagttccgcg tttttcaggg gccttaaata aaagtcaaac tgcggcaaca
    13981 tcatatcata gtcgccgctt ttaagcatcg gccagtaaac cagccgctga ttttgggctg
    14041 taaagctacc tcccccccat cggcggtagt ccggcgtaca tgtgatgcgc tggtcaacat
    14101 attccggatc aaaggtaaaa agtcccccat tgaatttggt gggatattcg ccgtaggcat
    14161 tgcagccgag ctggtagcga aacagctgat aattcctgcc gattgtccag ggaaggctgt
    14221 tgctggattc cttggaaggc tgtatcgcga tataactgcg attccagaat tcatcccacc
    14281 aagccaatgt tttctcccga ccgtcgattt tttcatagat gttaacaagc ctatcgagcc
    14341 cttgctgcca caattgctgt gtttcggcat ggtcgatgtg cagatagatg gcgatggaat
    14401 gagaaaccgc cggtttgatg cttttgagta tccaggattt gaagtccgta ctggcgtagc
    14461 ggccttcacc agtgccggct ggttccatat tcccaccgca aatcaagccg ccggatatga
    14521 gatttttaag cggattccac atctgatctt ttacatctgc caaaccctgc tgctcgacac
    14581 acaagtcaaa tgcattcggc ttgttattgt cgttgcagtg atagacgagg ataccatcat
    14641 cccggaatgc gattgtatcg ttgcgaacaa gggccttgac tccagagccc aagaatgagc
    14701 gattgctgtt gttttcctcg tcagcaagtt cacggggagt tgtccgccag gattcataga
    14761 ttgcttggac agatatcggc tggctgcagg caatatctac atgaatgaca ggcttataga
    14821 catccaccca gaggttgatg gttgcggtgt gttccccttt tttgcagact atttcgatat
    14881 atccttcttt cagtttcaat gtctgacgaa aaacagcagc ttcctgcagc agattgggtg
    14941 acagtttcac gcgcatccgg ccaaatttca tcaaacagtt attttcgtca taagcaccgg
    15001 tttgcgacac ataaaataaa atatcgccgt tttcgaccca aacattcaga ccaatgttac
    15061 cgccgccgca aggcatggac tggccggcat taagactcgg actgttccaa atcaggttat
    15121 atgcatccag atcaagaccc gcctgcacaa gaccttgaaa tgctgttaaa aaaaaagggc
    15181 aggcaactgt gataaaatat attgtttttt tgatactata catccttgcc tatcacctta
    15241 ttgcagttgt cctgctaatc aatcctctac taatcgggta aaaccaacgt cttactctgc
    15301 ctgcctgtac ctctttgcgc cgtcagaata cttttaatcc aagtgaaata cctcctccat
    15361 gcttgcccac cattctcctt cggctcggtc cggaaagggt tcatgacaag gcttgcatac
    15421 gtcccaccac ttctgtgttg ctgcatcagc ggccattttt ttcatatccg aatcgaaatc
    15481 ctgacccaca tattcaaaat agctgaataa ataatacttg ccatcaggca gtttgcgcag
    15541 atagatggaa tagttctgga tattgcactt tttgatcatg ctgagaactt caggccagac
    15601 agctgcgtgc agttttacat acgcatccaa tttcgcttct ttaacttgaa taacacttcc
    15661 gtaacgtttc atgtcagtct ccgaaataag ccggatttgt cgataaagca aaacgccctc
    15721 ttgacagctg catcgaatcc gttagtggca ataattataa gcagatttca taaaagagtc
    15781 catgcttttc agtgcatttt atacttgatt ccccacgata gggaattcat gttgaatttt
    15841 aggataaatt ctttcatgat ggtgcatcag aatatcatcc ttcaggttca tttcttattc
    15901 cataacattt taaaatgaat ttatagccgt ctctttgagg aatgtcgctg catcttatta
    15961 taatgttcct gtgtcatttt atcttacagg tattccgaac ttcattggtt tggcttccgc
    16021 cctgttggct tatcagaagt ttcatatagc gattggtcat atcagaaacc caagccaagt
    16081 actcatcgac cggcagcaag atttaatgtt agacatatcg caaaatcatt ggaactcttt
    16141 ttaaagatgt tgttttggtc ttatgataaa ttgcagcaac aatccggtta acaggatcat
    16201 tagagttcca aacaccggta tcaagaaagc atgaaaatgg ctttgcaggg aaacgggcca
    16261 tgattttatc ttgggagaca acgtcatcca tgcaataacc agcaagccga atacgaccgc
    16321 agccgctgca atcgagctat tgactgttcg actgataagc cccagcaaaa acaaacctag
    16381 catgcctccg cccaatatgc cggataagat ccaccatgca tccaaggcac tggtcacatt
    16441 gattaacagc agagacaatc ccgtccccag cagcccgcag aatatcgtag ccgcatacaa
    16501 cactgtcata aactgttttt cggaagcgtc aggccgtata taccgctgat accagtctgt
    16561 tgcaagcagt gtcgccgatg aattcagact ggtcgacacc gtactcattg ctgcagcaaa
    16621 aattgctgcc accaacaatc ctgtaacacc ggcaggaagc attcgcccta taaaatgagg
    16681 aaatacccga tcgccgagat cttgatctat cagtaaagaa gcccggttcg ataattccca
    16741 agaaaattcc ccttggtccc tgctgaaacc ttcctgaatc agctgctgtt gggcaacctg
    16801 cagccggact tcttccagat aatccggatg gaccttataa aatgcaaaaa gactggttcc
    16861 aataaaaaag aaaacagcag aaacaggcaa ataaagaagc cctcccagcc agatgctttt
    16921 acgtgcttcc aaatgatttt tggaagcgat atatcgctgg atataccctt gatcgatgcc
    16981 gaaattctga agattgataa caatcccgta aaccagaaca acccaaaagg taggttgtgc
    17041 caatgaaccc cccacacttc ccatgctgaa cttatgatgt tcggcagcaa tctgaaacac
    17101 ctgctgcggc ccttggggca tattgaacag tatcagcagc aaacatagaa ttgccccgcc
    17161 aatcagcaca accgcctgaa atgcatcggt ccatatcacc gcaacaattc ctccgataaa
    17221 ggaatatatt gttaccgaga taccggttat cagtataatg aggcggatgt cgcaatccaa
    17281 tagaatactc attggcaggg ccatcaggta cataacaacc gcaatgcggg ctaattgggt
    17341 caggatataa aaagagctgg catacattcg tgcccaggga ccaaagcgat gttccaaatg
    17401 ggaatatgca gatacgtctc cggtttttcg gtaatacggc ataaaaaaac gcactgcaat
    17461 ccacgctgca ataggcagcg ataagctgaa cacaaacgga ttccaattgc ttgcatatga
    17521 ttttcccggc aatgcaagaa aactgatact gctaagatat gttgcgaaaa tagataaacc
    17581 acacacccag cccggcagcg ccctgcctgc agcggtaaag ccctcccggg accggctttt
    17641 gcggtaaaaa taaaaaccga tgcacatggt gcttaagaaa tatacagcca aaacaatcca
    17701 gtcgatgggt aagaaatgac gctgcatgaa gtctcctgca ttcaggcatt gcttttgttg
    17761 aatatcataa gaaatccttt atcacttttt taatgaaact tcgcaattcg cagaaactcc
    17821 ctgtcaatca gtttggccat tggctgtaca agagcccata gaacaactgt ttgcttctgc
    17881 tattcatttt acggcgtcca aggagaacga aattggtatt tccctgattt aactttgtaa
    17941 acaatcgttc ctgcctgctg ctttagaaat gtaactccct ctgcttgttg tgcagggatt
    18001 cctgattcca atactttttc ggcatcttgg gcaggaataa atacatcagc ttcggtatta
    18061 ggaggaatcg ttacatccag tacaaaacaa ccattcctga tttcccaatt cgatacaatc
    18121 aatccccgaa cagaatgata ggatgttttg gcccacatca tttttccatc cggctgagga
    18181 tgaatcatca tagtcttaaa gcccggtcct tgggattgga tacctccaat attctcgaat
    18241 atccactgac cgaccgctcc gaatgaataa tgagcaaacg aattcatccc gggatcggca
    18301 aaaccgtctt ggggagtcca gccgttccac cgctcccaga tggatgtagc accctgttta
    18361 atagtaaagc cccaagaagg aaaactgtcg ttatagagca gccgataggc aaggtcatta
    18421 cgcccaatct tggccagcgc cagcatcaaa tccttcgtgc cgataaagcc ggtggacagg
    18481 tgatagccgc actctctgat tcgccgaaca agatgctcag cagccaattt ctgatgagct
    18541 ggatccagca gatcatacgc gatggccaat acataggccg tctgggtgtc ccccttgatt
    18601 gttccatccg gtcccacata tgcattgctg aatgcgctct tgagctgttc aaacagagtg
    18661 ccgtaggtct gagcatcatc tgtcttgcca agtgcatcgg cgatcatggc aaaaaggtga
    18721 gtgctatgcg caaaatacgc tgtatatata acgtcatgag gggtttcatc gttaatattg
    18781 agccaatcgc caaaacactg aaatttttca ggcggcaaaa gagcacttgt gcagcgtttt
    18841 cgattgaatt caataaattt cttcatggca tcataatgcg tctggaggat gcgcttatca
    18901 ccgtacattt tataaatcgt ccacgggcaa ataacaccag catcagccca ggccggaccg
    18961 ccgtctgtcg gatcgacttt cagcggggca tagcgaggaa attgtccatc gtcgcgctgg
    19021 gcgtcagtca gatccatcag ccatttggta aaaaacgact gtacgtcatt attataacaa
    19081 gccgtattta tataaacctg ggcatcacca gtccagccga atcgttcatc tcgctgggga
    19141 cagtccgtcg gaatatcaat aaaattcatc cgctgcgtcc agacaatatt ggagtagagc
    19201 cggttgacca ttggatcgga acaatcgaaa ctgccggcta tgggagtgtc actggtcaaa
    19261 accaatccgg taaccgtatc caaatccggc ttttcatcca atccggttat ttctacatat
    19321 tgaaatccat gctgtgtaaa atacggctgc cagacttctg ttttcccgct gcctttgcag
    19381 atataaatat ctgtcgctga tgccgttcgc agatttgttg tgtaaattgt gccgttcgga
    19441 tttaaccgtt cagcatgacg cacttggatg cgctgacctg ggcggccgtg aacctgaagg
    19501 cgaacaactc ctgcaaagtt ttgacccatg ttaaaaacat atatcccgcg cacaggttcg
    19561 gcaacagaaa ccggcttgat gctcgaaaaa accctgaccg gctcggtcac cgctgcccgg
    19621 atttgaggat tcacctctgt atctccggca atcaccggcc gccatgcaga ttcgtcaaag
    19681 cccggcatat cccaacccgg cagctcctga cgggcatcat agccctcgcc ctgcagcaaa
    19741 tctgcatata aaattggccc cgtcgatgcc ttccagtctt ttgccgttcc aacaatttgt
    19801 ttagtcccat cctcgtattg aatgttgagc tgcagcaaca tgcgtgtctt gcggccataa
    19861 tggttccggt tcagccctcc tccgacatag ccggcatacc agccgtctgc caaaatgccg
    19921 ccgatggcgt tgctgcccct tcgaagctgc ttggttacat catacgcccg ataatatacc
    19981 cgttttcgat agtcggtcca gccgggcgac aaataatcgt cagtgacacg ctgaccattt
    20041 atagaacact ggtaaattcc cagtgcagtt gcgtaaaggg tggcacgttc aatttcttta
    20101 ttacttactg caaaatcctt gcgaaaataa cgagctgggg gaagcagccg cttcatcggc
    20161 tttgtctgtt cccacggtgt tattccgagc ggaccaatct caacagcacg ggaccagccg
    20221 gcatcgtcat aatccaccgt tttccatccg agctgttcat ctgtgcttaa cagccattca
    20281 ttatcggtcg caaattccaa gattcggcca tctgcagatt caatccggac agccgcccaa
    20341 aaaccggctg gactatccgc atttccttca ttgacggcac gaaacgctaa cacattcaca
    20401 cccggcctaa ggtaatcagc cagcacaact tcgtagatga ctttacgatt gcggaatgac
    20461 ctgagcaggc tgccgttgag atgcagctgc acaaaatcat cggccgtgta gtagcatacg
    20521 gctttttgaa taatccaatc ctgtggaagt tggaaggctt ttctgaaata acaggttccc
    20581 ggcggggcgc tgatattggc gttaggttgt gcccataccc attttgcacg gctcacaaca
    20641 tccggcgtcg gaatcggttt ctcatcgata tcatatccaa tccattcggc ttgccactct
    20701 tcctgatgga gcaggcccat agaccatttt gcaggcagac tccatgggga cgaattcccg
    20761 tctttatccc aaagacgaac cttccagaaa taaacgctgt cggatgcaag cggtttccca
    20821 gcatacgcaa cggcagctgt ttcatcactt tggacctttc cagaatccca catatccggc
    20881 tggttgtttt ggagaagctg caggtcagat gcaacataaa tctgataagc agtctgtttt
    20941 tgaccgcgct ggtcggattg gagctgccag ctgaaacgag gagatataat gtctatacct
    21001 attggatctg ttcgatattc acatcgcagt aaaacgggga tgagctgatg agaagccgga
    21061 ttcagagaac tcaagcagcc ggcacagaca accaaaccaa cgttaaaaac gattagaggc
    21121 atgattcgtt tcattgtaga ttttccgctg cattattatt gaagttttac tgttaggtgc
    21181 gacagctcat cggcatgtcc gatttctttg ctgtctgcaa aaattcgaaa tccctttcct
    21241 tggttatatt tttcaccggt tcggtcccac aatatcgtaa ggatttttcc atgatagact
    21301 accctgtcca gacagaacca atcccatgcc ccatcgggaa gaagcggatc aacctctaca
    21361 atgtcatctt cccgaggcag taaaccgacc aatcctgaaa tgaccagatc gcaaaaagtt
    21421 gaatgattgt agtagcggct tcgattcgaa tccggtgtca gccagatacc gctgatttca
    21481 tcgaggtatt ccccgatata aggtttgccg ttgcgctgat gcgagcgggc ataagtcaac
    21541 aaggcctcaa agtagtcttt tttgctgaca tacgactggc tataataccg caggaagttt
    21601 gacaaggccg tcagagtctg gcttgttgcg aacggccaaa cagcaccatc ccattcacag
    21661 gttccgaccc catgactgcg aaattcagga tgccgccgtt cggcggttgt aattcccata
    21721 ggagctttaa aaccctgcgg atccgtgaac tgctgccagg ccgcttcaaa acctttctcc
    21781 ggcagattaa aataccacgg aatatagcca agttcctccc gaacattagc caatgtgccg
    21841 tccggatggc gtactttgaa aaacgcggat gtaccatccc acatcagctg ctgaatccgc
    21901 aacttcaagt cggatgcttt ctgaatgtat tcttttgaca gctcaagatt gcccgccacc
    21961 tcggcaattt tcgcaatagc catagcattg gcgtacatat agcagttcag cggaggccgc
    22021 atatttttaa ttgttcggct gccgctgatg gactcttcca ttccatcccg gacatcgtat
    22081 tgccagaaaa gcccattctc cgcagctttt tcatcaaccc aggcttgata atcctgcaca
    22141 aaagcgtcca gcaggcctat gataaacagt ttatcctgat taaccaaata ccggcgatat
    22201 aatgctgccg tagcccagtt gctgtatttg tggaaatgcg gctgcaaacc gccgttatgt
    22261 ccggtgtacc agaatcgggc atagtcatca atgtagcggc agtctcttag ccatgtccct
    22321 tcatagatat gatgaccaac tgcacagctg atcgtgttgt atttgccgct gtgaccaaca
    22381 ctgtcaagaa attcggtgaa gacgtagccg tccggcgtcc gttttaggtg tttgcgaaaa
    22441 gtccaccagc gataatagta gatttgctca agtgttttat cggggcattc gaagaggggg
    22501 acattttctt tgatccaatc ccaagattgg gcgtttggta tttcattgac aatattttct
    22561 tcttccatgc tgttaaaatc atcgatatag tgctcaaagg tcctggggga taatacagac
    22621 tgtcccgtat caaaggcacg gaaatcttgg atgtaatata ctgcagcttc aacctgttca
    22681 tcggctcccg gttcatctgt acccggtata cgctcgctgc cgcttttgta tttttgaaca
    22741 tcatcggcca gccgataggg gccggttcgg aaaactattc gttccggcag ttcttcgacc
    22801 gatgaaaaag aagcatcttg tacgatcata tttccatcca atagaacaga atactgctgt
    22861 tttggcgcat caacatgaaa ttcaactgaa taccattttc ctgaattcaa tgccgcaacc
    22921 gtctgataag gttggtttcc attttttgtc agcagacgac cgctggtatc cagccgtgtt
    22981 tgaaccagac ggctgccttt tgttgaaaca atctcgatat ccagaacttg tgcagacgat
    23041 tcaatataca gctggaaaga acatatcaga ttggctgatt tctgaaaaac ccttaccgct
    23101 ttggcataat cataaggatc ggcatccttg atccgcatac ttttgtcagc tttgctggga
    23161 aaatctacaa cttctattgg acaccattgc ggagaataga tattccaacc tttaataaca
    23221 cccccagtgt ccatttggct gaaatcatcc gcaacagaac cctgcacctg ccattgaaca
    23281 ggaactggaa cacgggatat ccagatgtct tccttgttca cggaatatac gacccataag
    23341 tcgctgccgg gcggattgcc attgccttca acaatcccgc gtacatattg gggacccgga
    23401 cgtttttccc gaccccagaa ccgcttggga ggaacttcgc tgtgaatgtt gacaagatgg
    23461 tcataatgta tcgcatcctt gctgacggcg atgcacagcg gatggcgggc ttcgctgtcg
    23521 gtaggattat aaaccaaggc atattggccg ttgtccagtt tttgtgccca gattttagca
    23581 ctgccatagg ttaatgtgct gcactggacc agcggagacc aggtttgtcc ctgatcacgg
    23641 gtcatcgtaa cccaccggct tttgaagaaa ccgaccaccg caccatccgg tcgtgtataa
    23701 aaacaaaatg ccttggccgg tcttgagcgg cctatatcac cgccccatgt gacgcggtaa
    23761 aagtcgtctc tatctttggc ttgataatcc tcttcccacc actgaatcct tcgaatttta
    23821 tcttccagaa agctgcggca ggcctccaca aatccggcat caggtgaagc ggtatagaga
    23881 ggatatttca cctcgcctgt ccagttgtca tttacgcgaa taaaataaat cggtcccaat
    23941 gaattatccg catatatctc ccggacaacc cgacccactc catccccatc aggcgatcca
    24001 taaaaagcca tcgtcaaaaa ccgcccattg tcagccaaat aaaaacccat tcgctgatgc
    24061 ataaatacaa acgttatacg agttgcatca ttcggatcag cggtaaaata aatcggaaac
    24121 agaacctgcg gcttggtcca gtgctttcca tccaatgacc gtgtaagcat cgtcataccg
    24181 gggggaatat gctctccgca gggattcgtc aagtactgac aataaaactg cccgttgaca
    24241 taagccagca tcggtgcatg attataggtc caacccaatc catcagagaa ctctggatgg
    24301 gttcgattgg cccgcaggat ttgataattt tgtacgccga tcgctaccgg catctgaccg
    24361 tcatgatagc cgtttttgta atcgctgttg gatactttta cctcccccac aaatcgaaca
    24421 ggttcctctt catcacctac ggctgcccaa agagcagagg ccggcaacaa aatagacaac
    24481 aatctcacta taactagaaa ttgcctggtt ttaagcatat tttgaaacag tcctttctgg
    24541 caagggattt tacccctacc ccgttcaatc gactcgacgg attctcaatg ccatataggg
    24601 tttgcccggc aagataattt ttgctttctt tttgtcaata taagtatatt gattctgttt
    24661 ttcgatttcg aattcctgat tgacaggagt aattgtcata ttccaagtat caataatatc
    24721 aacccgaaat cgcatgccct ccctaagacc cttctgcggc agtttatact cccattctgt
    24781 caagggttct ttgccaaaat aaatcagata atagttgccg atctggccgg ctatatttgt
    24841 ttcaaaatac ggatcaacag gatccagtcc ttccggcgga ccttcttcgg ctatctttct
    24901 taaaaaagca atgcgcgggg ggctttggcc cttcaagtcc ccacccacag agatccaatc
    24961 tggatcattc ggccgctgaa ttgtttctcc atgggttgca tacgcacccc cgatataagc
    25021 aatccaaaac cgataaacca tttcttctcc ggacagctgc ccccagcggc gatcacggtt
    25081 gccttcgtaa ttaatctcgt catgaataac cggtttttta taaatatcac gcagcagagg
    25141 agagactccg aaaaaccgga ccgccatata atattgcaga ctgacatgtg ttacccaagg
    25201 ctgggtatga tcgtaaatcc ggtctgcatt atggatagaa cgcaaatggc cgtacggatc
    25261 gctgcgctgc aaaatccgga aaaggtggtc ccagtcctgg tctgtcagag actccataaa
    25321 actattttcg ttggcaaggc tccaccacac atgccgatac gcagcaaagc gagctaccac
    25381 ataacgaaga aaacgttcat tcgtggccat atccatggta tcaaatcccc atttcccgtt
    25441 atcataaggt cggaacagaa tcagttccgc ctgaaccccg attttaccaa gctctgcgac
    25501 acagcgctcc agccggtgga aaaactcagg attaaaacgg gaaaaatccc agttgtcttt
    25561 tgaattgccc acaaacggaa aacaatttaa ggcgtgaggc ccttttacat agtattcctg
    25621 ccagggaggg atcaccagaa agcgtgcttt gttaaaggat gcagttttca atgtttcaat
    25681 cgtttgcgtc tgcatggatt cagaataaaa cgcccattcg taaatggttg tgccgaacgg
    25741 gaaaaagggg gtaccatctt catacgcaaa atgatattga ttccgcacac gtactggccc
    25801 atggttgtcg gccgaggggg ctatacaggt aaactgcccc gttttgccgt tcaaggcatc
    25861 acagttgctt ttcgtctgat aggtccagag accttcgcta ttgggcataa aacgaatttt
    25921 aaagacgccg tttccatcat aaaaaccttc cggctcaaag acctgatcgc catgcttgaa
    25981 aacagccgta aattgaattc caataaacgg atttccagcc gcaggtccgg acagcgatag
    26041 ttcataaact ccccaccgtt cgacattttc atctgcccca aagcagatac agtgatacat
    26101 tattcccaac acgacgagac atccaaacct gttcattctt gtctgccctt tacagaatac
    26161 tttatttatt gtcgccgaag ccataataga caaggcgtat tctctaattc catctgcacg
    26221 atagtgccgc cggagacatg tttttgggtc tgttcagatt gtcctgtctt caaattgata
    26281 tacgaaacag taaaatcagc atggatactg ctaaaatcaa tcacaacagt atctccggaa
    26341 tcggcataaa tcagcgcatc tcctgctgga tccatcagag catattgccc ctctggattg
    26401 cccgccagat tatacggatt catagaagga actgctttga gcaaatcctt atctgtcgtt
    26461 gagggaagat tgggaataga ccctccgccc attagaaccg cccatccgta attggcatca
    26521 gccgaataaa tgacggcctt gtccgaacag cgcgttctgt attcccggac agccctaaca
    26581 acttgtgaaa atgaggtgcg gcgaggatgc aggaggcgtg catgctgccg gggagccaga
    26641 tgctgtccgc cttggggggc atataacagg ccgtccccct gataccacca gtatcggatg
    26701 tcgattaggg agataacctt ggaccgttta ggatcagaca atatctggtc ttgaacatcc
    26761 ttggtacagc taagtccaat taaaggcgac cgcccggctt tttcctgcca atcaataatc
    26821 gtatcaagcc agaactggac gaattcaacc ggacccgtat attcctggct gatcatctga
    26881 attacattgc tgttgtctgc aaaattccca aggcattggc gaatataggc ctgatgcaat
    26941 gaagttctga ccggatgcct tgtatcgtaa aactgttcat ccatgtaaat gcgtttatcc
    27001 ccggcataag ggggcggttc gggaaaacct gtatcgttga tattgttggc ggatcgccaa
    27061 ggaaaatccg cccagtgtgc ggcagcttcc aggatgttat gctgaaaata gttgtgatga
    27121 atcaaaacca aacccttgcg atcgcataga tcggcaaatt gctttaagcg attccaatac
    27181 cacacattat attttgtcag atcgtaccgg cttaatccat cccatgcggt tccaactccg
    27241 cttctggcaa acggctgctc ataaaatggc ggccgaacat cgccgttcat ccgccggaca
    27301 tgctgatggt catcgcggcg ccggtcatac caaagtccgt agttatgttc cagaatcgcc
    27361 tgtccgtttg cgatcattct atctgtcagg tcgtttaagt catccgtata gccgggccca
    27421 tttcgtccgg ggacgaatcg ggttattccc actccgaatg atgcggccgc atccggtcgg
    27481 atattgccgc gccaccatgc tacttctgtg gatcctccga taacaagctg attatcgcaa
    27541 accaatcggc cgtttgagag ggcaatggtt ttgccggatg tattgctgtg attttcaggt
    27601 attatgctgg atatgtcttc tattcttttt gcatcggtgc tgtcggtggg aatcaactgc
    27661 cgtttgggag ctgcttctat atactctcgc aaagttactg ctttcttttc ggagtgtgcc
    27721 gtcatttccg ctgccaattc gaccgaagga ctcgttgtgc tggatgtttc atttttcata
    27781 agggagatag actgtgcctt accgcccaac cgctgctgca gctgcgcaac atagagactg
    27841 tgcggattga taaatgagtt ggactcgtgc cagaaaccct tgccctcgaa ctcgccccag
    27901 cagccaaaag cccaattcca agccgtcggc ggcttatcac attcgataac ggctgcctga
    27961 cattgccaca aaacgctgtt tgcagccgcc cagccgatgc cctcgccgcg actgccgcga
    28021 tttccgagcc gcagggcatt gccgtcaata cgaacgttgt catacaaaac accgcatgcc
    28081 cagctttcaa ttggcccgct gtcttccagg gattggaaac tttcacattg tacaaatgca
    28141 ttggggccgg ctgcacaatg gccgacggaa aaatcatgcc gacccttctc ggaccagcag
    28201 cgaaggaaaa gcgtttgctg gcccatagta aaaaaggtat gtctgcgata accgccctct
    28261 tctgaaatcg gagccagtga aagacaatcc tctacagtaa tacgtctgca tgattcccaa
    28321 actgcaacag ctgatccggc aaaatgctcc attgcaacct gacgcaccca aacatcttca
    28381 gcgttctcta ccgtcacggc catccatgcg tggtcttcat ctttgggatt gtcaagatta
    28441 tattccgacc gcaagcaaag attttctact cccaccttac agatgcgtcc cggccagtta
    28501 tatgtctgca gtgttgcttg agggttgatg gctgcagtaa ggggcgcatc aatcgtgata
    28561 acaagatcgt taacatccgt tataacccgg tcccaaacaa tattgcgtga accgggtttc
    28621 cagtcaaaaa gtccctttaa gccccctcca aaacgattca tttccatctc ttctatccat
    28681 tcttgcgtgg agggacgtgt aatacaaata ttatcccctg ctttgagtcc ttttgtctca
    28741 tccaaacgga tatgataggc gccaaccggt acaaattcat cggcaacatg ccgaagtctg
    28801 tcagaatgcc attttcggtc attgatcccg gcaaaacgca gcaatgtccg gcggtttgtt
    28861 cccgcagcga taagcacagt cccatctgaa cccattccct cgccgcggag gacaactcca
    28921 gaggaattaa ttgttagacc gccccaaacc gaatatttac cccgccgcag caaaactgcc
    28981 ccgcgaatac cttccgaatc ctgcggaagc atagcgacat aatctaaagc tgcctgaatc
    29041 cggccggtat tatccccctt tacaggtgaa agaacaaccc ggatagtaca gtccggaata
    29101 gggtgattgg ctcctctata gccgcaatgt gaaaaatcag gaatctggtt gcctttctga
    29161 tctgtcccgt agaccaaatg accgtttttg tcaagagtga gaaaagaaat ctccggcaca
    29221 tccgcacaat atcccgccac tgtccacacc aaaagcgcta tcagaacaaa tacccctgct
    29281 tttttcaatt ctgcttgtgc cattgtcatt ccgtccctgc ctttgcagtt tttgcagaat
    29341 cctgacggta aataaatata tctggccttg ttggttttga taagacccta cttgactgtt
    29401 tttttcagtt gtttcttcat agagatagca taattaaaaa tgggagaatc aatcgacctt
    29461 atttcaactc ggacgattct acgccggtac atccaaacat tagcagaaag attgtcatcg
    29521 tggataggca ttcttcggcc attgtccgtc gggcatcggt acggtttgga gtttctctgg
    29581 attcagcact acatgcttga tccgttgacg atgccaagta tatacaatgt gtaccaatcc
    29641 gtcagcagtc tgaatcacgg atgggtaaga aaattggctg tttggctggt cttcgtattc
    29701 aaggaccaat gccgcctgcc aatttttgcc atcgcggctg agcgcaacat tcaggattcc
    29761 gcgccctttg tgccccacag ccggctggct gcgagtcgaa tggttataaa caagcagctg
    29821 acggccgtct gccagtgtta cgccgtcaat tcctgagttc ggattcggca gtattgtcgg
    29881 ctgcattggt gaccagtttt ttccgccgtc tccggaccat gtttctaaga ttctatcctg
    29941 ccggctgcgg caaagcatct ggatttgtcc attgggatat gtcaataatg tcggctggat
    30001 agcggacatt tcattgggat cgttcagcgg cccggtcagc tcagtccagg ttttgccgtt
    30061 gtcgctgctg gcctcaatat gaactttcca cccgctgtct tcacggctcg aaggggccaa
    30121 aatcgtacca tcggacaatt ggatcggctt gtttttgact gccccaatca gtgccttgcc
    30181 tttttcaccg attatagttt tgggcttact ccagttccaa ccgccgtcgg aagacgtcat
    30241 catcatgccc caccattggg tcggggatgg gccaacttta tagaacagta gcagttctgc
    30301 acctttgggc aaaaacagaa ccggattcca agttgggtat cgcagttcgg gtgtaattgt
    30361 accattggca atttcgacaa ccggagtcca cttgccgctg aatgtgtgac tgatccagat
    30421 gccgacatca ggattgcgtt cggccgttcc gccaaaaaag gcgcagacta actccccatt
    30481 gacttcggcg atagttgctg catgacaaga gggaaaaggg gcgttggtaa aaacaaattc
    30541 ttcaagcacc agtcccggct tgaacgaagc aggttgctgc tggcacaact cttgtccggc
    30601 agttgggatt gtagagggtt gcgtgctatc tgcctctttt gggggttcgt gggcactcac
    30661 agcatttaag ctcaaaacaa acaagaaaat acacagcctc aataatattt tcatcatagg
    30721 ctcaaaattc cagttccgca aaaacattga cttatgggta ctcgaaaacc acaagaaata
    30781 aaatgcatcc tgtcaaatat aaagcttcta taaacctgcc aagcaatcag gcgcgcaaag
    30841 cagctaatca tttcagcggc gataagattc cagcttatca gaaagctgtt ccatctgcca
    30901 gggacgaatt atctgtccgc cggctgaaat gacattcaga tacacacctt caggcccgat
    30961 cgcatccaat aatggaataa catcatccgc atcaagattg gcaacccaaa cggcctttcc
    31021 cgcacttcgg atccttttat acaattcgta ccactgagac gaccctgccg gcggaacctg
    31081 agaatccggc gtccattcaa tggcatccaa tccctcaatt tccagcagtg catcgatatg
    31141 tcccagacat tgatgtccat ccacatggaa catcgaataa tccagatagc tgacggacca
    31201 tcttaaatac ggtacaacaa aatcttgaaa cattttaggg gaaaacagcg ctgctacatc
    31261 acactgaagc agacctgttt taccccgccc ccagagcata aaataaccaa aagccatagc
    31321 accgtcttca tctttgataa tttcatacag actatcaaat acctgccggt aagcgatttc
    31381 gatttcctca agtttttcat gcacccacgc aggacgttcg atcatatcta tcaacaattc
    31441 ctctgttccc cgcagctggg ccagcacatc aagattggga acaatggcag gcattcccac
    31501 aaaataatgt ccttgactgc ttgcgaccgt tctcccaagt actgccttca gcatccggct
    31561 atagggatgt gcaggatcat acaaaagtgg gggatgcgta tcaggatcag caatgcaggg
    31621 tcgataccag atgttatcct ttttatactc cggaacagca cccagataag gggctaaagt
    31681 aacagtgccg atatcgggcc aggcaacagg cagcacatcc gccggccaga ctttgtgcgc
    31741 catttcataa tgaatgcgtg cggataccaa ctccggatcg gtatgatatt gctggaaatc
    31801 ggccgcaggc ggaggcagaa tgacatcttc atgcggcata ccatcactgg ctattccagt
    31861 gccccagcag cccagcacaa atcctttaca cttccaccag tctgtaaaat gctgttgtgt
    31921 ttgcagccag ttttttttcc agagtaattt catctgataa tcttcgttgt taatcgtgct
    31981 tgaagccggg tttgatatcc tgataggccg gctcaagacc agcctttttg ataatcgcct
    32041 ctgcctgctt ggaccatggc ggggcattcg gttccacaat cgtgcgagta atcgggcaat
    32101 tggtgccctc acagcgaatg ttgtcggccc taacataatt gttgtcgatc ctcatatcat
    32161 ggcaggcctt tgtccagata tgcagccaca atacagtgtt ttctgaagag ctcgaaagct
    32221 cgtttaaaat aacattatga tgaatcttcc aataggccga tccgtcatcc ggataaatgc
    32281 accgaggacc gtcatagcaa taattatatg caatttccga atccggctgc tcgcccagca
    32341 cataaataat tcccccatct tggagcacac ggtgcgtgct gccgatgcgg ttgtacatga
    32401 tagaattatt ctttgctacc gtcgaggggg gaatctgggc attgccccac caccagccca
    32461 aagcaattcc gccatagggt gtaccgcaaa tctcattatg tacaattttt acattttcta
    32521 caaaaaaacc ggtcattcct tcgagctgtc taaaatcgag gcaggcattg tggacatagt
    32581 tattggctat cagaatattc ttacagactc cttcaatccc gcgaggaaac tgctgcccat
    32641 cgccgatctg gtaatgctgg ggatggccta tattgaccga cgttcccatc aaatcaccaa
    32701 agtaattccc aacaaccgcg gtattaatca catcattttc cagatttaat gccacagcac
    32761 ttgccaagtg ctcgaaacga tttcgttcca accgaatgga gtcggcattg cacagagtga
    32821 ccgtaccttt tggaatggtg atatcattat agcggttcgg atgccagttg ccgttctcga
    32881 tatatcgcac aaattgcgat aagctctgta cgctgaccca tccgcgtgaa tcgcccagca
    32941 aggataacaa gtaatcatca taagcaaatg ttagtccgta aaattggata tttcggaccc
    33001 gcaaggatgt attctctcct tttatgctga caagtccttc tacggcaggg gcatacacct
    33061 gtgctgttgt catatcctcg cccctcgaat aataatacac cgtatcggcc tgatcgtcga
    33121 aataaaactc tcccggctca tcaagcagct caaaggcatt ccgaatatat ataggccgat
    33181 ccggacgcgg atccgcatga aaaggcaccc agctcatatt caatgcaatc gcaccatacg
    33241 gctgctgaag catagcgatg gtatatccgt cctcgtaaaa catatctttg acgcataaaa
    33301 tcggttcact ccatatattg gattgacata attcgacatt ctgaggatta cggtatactc
    33361 cgacttggtc cgatttaaat ttgatgccgg caaattccgt cccagaagtt gaagcccatt
    33421 cctctttacc agtgatagta aatgtcccgt aaccgccttg cccgataact ccgcgggaca
    33481 tccctgccat cattgctctt ttcccattta tatacaatgc ccgcagtttc cggtctctga
    33541 caagccgcgc cttccagata ttacccacat gatgttccca gtccctaacc ggtaccgctc
    33601 cgttcaagat cggtttttca ttctgataag ctttgtaaat gatccgatat ccgttcattc
    33661 cggagtccag actggtcaat tcgaatgtcc tgtccagcgg atatatcccg ccccgaagat
    33721 ataccacaat atcgtggtgc atattttggt tgatggtccg gataaaatca cgagcatgct
    33781 gcagtgtccg ccacggctgt tccagactgc ccgctgcact gtcatcaccg ttgggcgaaa
    33841 tataaaaagc cgtttgcggt ctttgaaatg tggttataca accgaaaaaa aacggaagga
    33901 ctgcacccgt atatgcaagc atccatatcc tcaaaccggt ttctttgcgt ttattcatga
    33961 cgccttcatc ccatactgaa agaataaata caaacctacc ataataactg ccagaagaat
    34021 ccacccggcc aatccgacag gcccgagtcc cggattttgc cgatacgtct gagccagcgt
    34081 cggcaatggc gtttcagaaa tgctgtgttg tgttaaaaga gaggccgcta tcataaatac
    34141 gaggatactg cagaataaat aaaacgatag cagaagaaaa tgcgggaaaa tatcttcgcc
    34201 ggcttcattt gcaaatatgt tcgtaatatc gcaaaagccg atcaccagtg aaatcaatga
    34261 accgacgatc aatgtcagca aagctgctgt tgcggtcgaa cgtctccaga aaatccccag
    34321 cacgaaaacg gccgataccg gcggagccat gtatccgatg ataccttgaa acaggttaaa
    34381 cagattcgtc ccttctgcct tgctcaaaaa caaggcaatc catacagcca gcagagtcga
    34441 gaagagggtt gttatgcgtc caatttgttt gatttgatgc tccgatgctg tcggggctat
    34501 ccagcgttta taaatatcca gtgtaaagat tgtactgaag gaattgagac ccgaatccag
    34561 tgttgaaatc accgctgcaa ccagaactga aacaatcaat ccaaccaatc ctgccggcaa
    34621 gtaggtactt accagactca aaaacacctg tttgtcatct ttaatatcgg gaagcaactg
    34681 agctgcaaaa atacccggca gcaaaaaaat aaagggcgga atgatcttaa gaaatgccgc
    34741 aaataaggtc cccccctggc tttgctttaa attcttcgct cctaaggcac gttgaacaat
    34801 cgtctgatct gaacaccaaa accacagcga aagaacaggg tatccaagga caaacgcata
    34861 ccaaggattt tctgaaccgg ccggatgcag cagtttccaa gtcagctccg acggcgtgct
    34921 gccgcactga acatgaagca gggcagaaaa agattccaat tgactggccg taataacagc
    34981 cagcaaagcc gctcccaata tcattagaat tgactggaat gaatccgtta ccattaccgc
    35041 aactagaccc ccggtaatgg tgaagctagc ggcaacggca gccagcagcc agatacacag
    35101 catcaatttc cagcccaga
//
LOCUS       DAOTBO010000021        34705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1021_length_34705_cov_5.422136, whole genome shotgun sequence.
ACCESSION   DAOTBO010000021 DAOTBO010000000
VERSION     DAOTBO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 34705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34705
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_34705_cov_5.422136"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..259)
                     /locus_tag="PLE88_04310"
     CDS             complement(71..259)
                     /locus_tag="PLE88_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75725.1"
                     /translation="MEQYTGCRYAGIAGRIGSADCRCRTMFTGYEGVDHQFQKFWIVI
                     KINPEFLNLQTLPDLREN"
     gene            792..863
                     /locus_tag="PLE88_04315"
     tRNA            792..863
                     /locus_tag="PLE88_04315"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:Thr,seq:tgt)
     gene            908..1783
                     /gene="argB"
                     /locus_tag="PLE88_04320"
     CDS             908..1783
                     /gene="argB"
                     /locus_tag="PLE88_04320"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOM75726.1"
                     /translation="MEQAIEKAGALIEAMEYIRRFRDKIVVVKLGGSILDDCELQKNL
                     LTDIVFMSIVGMRPILVHGGGKAITAAMNEAGLEPVWVHGRRYTDERTLTIAEHTLIK
                     TVNQPICQTLQEMGIKAMGLHSLSSCVVFAEPLRLENGDGRKIDLGFVGKVSEVNAEL
                     LITLCGSGVIPVIAPVARAKTGGKLNVNADSVAGHIAAAVKAEKLVVLSDTHGIRRDV
                     SDPGSCISSISEKEIAGMIADNVISSGMIPKVEACLTALDGGVGKAHIIDGRIPHSLL
                     LEIYTDKGIGTQIVK"
     gene            1808..2995
                     /locus_tag="PLE88_04325"
     CDS             1808..2995
                     /locus_tag="PLE88_04325"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOM75727.1"
                     /translation="MKTQDIIEMYSRYVIGNYGRLPRVIVKGQGNKLWDLEGNEILDF
                     FPGWAVSGIGHCHPKVVEAIRRQAGELLHMDNTFYTLQQGQLARLLSERGFGGKCFFC
                     NSGAEANEAAIKLARRHTPREKYKFITAEKSFHGRTLATVTATGQPKYHEGFLPLPPG
                     FVYVPYNDIDALHQAFNEEVAAVMIEPIQGEGGIHEATPEYMQTIRELCDAHGALMIL
                     DEVQTGLGRTGRWFAYQHYEVTPDIITMAKALGGGAAIGAMMAKPQIAASLVPGTHAS
                     TFGGNPLACAAGIAVIEAIEQEHLLDNAHTMGDYLRTRLEQLREKYPIIDHIRGKGLM
                     IGVQLTMPGAKIVSRCLEKGLRINCTQETVLRLMPSMTITQPEIDKAITILDEVLAEG
                     QQA"
     gene            2992..3903
                     /gene="argF"
                     /locus_tag="PLE88_04330"
     CDS             2992..3903
                     /gene="argF"
                     /locus_tag="PLE88_04330"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOM75728.1"
                     /translation="MKSFLSIADCSRDELEALLTLSSELKRLYKSGGRDLCLSGKVLA
                     MLFEKTSLRTRISFQVAMSDLGGTAIYLKPEDIGIIGKREPAKDMARVFSRYVDGIMA
                     RTFAHETLLELDRWATVPVINALSDWSHPCQAMADMLTIQEHCGKLAGLKLAYIGDGN
                     NVARSLAFACAKLGMAFVCASPAGYELDKQTLEAANAVTAGCAVQIRCPEEAVRDADI
                     VYTDTWISMGQEEEKAQRIRDFQGYAVDRTLLSKAAKKVKVMHCLPAYRGNEITDEVM
                     ESEQSIVFDQAENRLHFQRALLKYLME"
     gene            3914..4552
                     /gene="rdgB"
                     /locus_tag="PLE88_04335"
     CDS             3914..4552
                     /gene="rdgB"
                     /locus_tag="PLE88_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOM75729.1"
                     /translation="MKILVATTNPGKLEELSMLLGDIAGRIEWVSLRDFPGLPEIVED
                     GATFADNARKKALGYAQATGLWTLADDSGLCIDALHGQPGVLSARFAADECPAADRKT
                     LDKANYQKVLRLLKDIPDSQRTARFFCHLALADDKQILLEATGAVEGVIINQPRGQNG
                     FGYDPIFYIPSLGKTAAQLDNSSKNQVSHRGHAIRQMKPLLEQLLAQRQAGR"
     gene            complement(4549..6822)
                     /locus_tag="PLE88_04340"
     CDS             complement(4549..6822)
                     /locus_tag="PLE88_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOM75730.1"
                     /translation="MRLHRKLWGGLFILAAFISSTLAAQEAAKPENLFELSLEELMNV
                     EITSMASLTSATRRLQPSAITTITEADIQASGARSLYELLDIYVPNLQWIRNWWEPDD
                     IGLRGIISDRDDKYLLTVNGKVMNDRTHYGAISERDLVLLRDIHHIDVVRGPGSALYG
                     PGAVSMVINIVTHNYKTFQGTEITTRAGVVEEFESVEIRHGREFSDNDGGIFGYAGIG
                     NYVGASKYDAPVHYGFDFPSVSWFDWTGQTGADYYPGDGTAAGDAMTSINMPRDQAAA
                     RGLPPVKLFGQINRGDWTYWARYTRGGQAFSWEGSTLARVPYGWTDWLRGGIVDWVEY
                     EPGQWGPAWDTYPLAQNFYQYQQLTGYAGYDTSLSEKTDLSLGFGYDMFDYNRFVINS
                     IADSYREDEYYARVLLRHRFNERHQAAFGAEVSHHELGLDPHGWPDIGFKSSNDMLSG
                     DNASRWSTNLYSLFGEYQWNITDQWTTFLGARVDDHTYSGKMFSPRAALVHTPNEKDT
                     YKLMWARSVRANFEEEMRAGALNGLGDGDPEKLDSVELRYERTHTKNIDLAASFFWHY
                     NLELISWSGSTGAPVPIGTQKNYGIELEAYYHTDKTRFGISHGFTKLYEFDLAEGQST
                     TLTSEAYGAGEDSALWSKHVTKLTACHKLTDQLTVDGSLRTYWGFSGIKDLERYRLSQ
                     NPNHPIDPDWERGYRGNYYLNLGLQYQPDKNLTFRVDGMNLLGIFDKDLNKRNYGSSG
                     AFRSHAPAVAVTMTYTF"
     gene            7212..9593
                     /gene="topA"
                     /locus_tag="PLE88_04345"
     CDS             7212..9593
                     /gene="topA"
                     /locus_tag="PLE88_04345"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOM75731.1"
                     /translation="MAKTGKSLVIVESPAKAKTINKYLGSSFEVKASMGHVRDLPAKG
                     LNVDIENNFEPTYEITPGRKRTVQALKTAAKGCSSVYLATDLDREGEAIAWHLKECLG
                     LSDENTFRVVFNAITKNDIQKAFANPGKIDMDRVMAQQARRILDRIVGYQISPLLWKK
                     VTRGLSAGRVQSVAVKMIVQRERQIRAFVPEEYWLIPGIFTTSSSCSLGADWKAFMES
                     AADKEKGRCLSEQQQWLAAHNAFKADLITVNGEKFQAKIAQQAEALVSALRESHYTVT
                     DKTTRRIQSRPSPPFITSTLQQAAANRLGFATKRTMLVAQQLYEGIDLGSMGSLGLIT
                     YMRTDSTHLSSEAISAVRRYIGSQIGQQYLPEKPNFYASSKDAQEAHEAIRPTDPDLI
                     PSEIKPFLTDDQYKLYDLIWRRFVACQMAAAQWDTTHVEMTGQKDGFSCLYRATGRVL
                     VFDGFTKIWPTSTEEPQLPALEKGDRLGIISIDAEQHFTKPPARYTEASLVKALEKEG
                     IGRPSTYAQIISTIQERKYVEQIDKKFYATDLGEIVTDKLEECFPRVMDIAFTRHMEE
                     QLDKIEEQHLNWVDVLNEFYRPFKDNLEKAFTTEHAKAQTQPSDYTCPDCKAPLVYRL
                     GKNGRFLSCSAYPNCKFACPCDKDGKMLQEKPSEHLCPVCSKPLVQKRGRFGPFLGCS
                     DYPACKTIVKLDKQGQPLPPKPPAQSSGVKCYKCKNGDLLIRQSKRGPFLGCSNFPRC
                     RTMVSMKHLDELKKLQAEGQWPPASEEEARRILGIDAAAKAKTSKKSATARQV"
     gene            9590..9922
                     /locus_tag="PLE88_04350"
     CDS             9590..9922
                     /locus_tag="PLE88_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOM75732.1"
                     /translation="MTKSDKNISITNKETFETIEPIGKRVLIRKDEDKKQTKGGIQLP
                     DNIEIPTITGRIVSVSAEVEMSSEFPLKQYDKVLFNPKGAIPVDFEGDNRLFVVEVEN
                     IVAVFRKQ"
     gene            complement(10074..10688)
                     /locus_tag="PLE88_04355"
     CDS             complement(10074..10688)
                     /locus_tag="PLE88_04355"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75733.1"
                     /translation="MNPRWSVIAGCVLVFLAVSAGVRGADENSYTLLIQSSPPDGGVV
                     SPGMGVLAMPVGQKIPLAATPNRGYRFAYWLGDVSSNGPADAIVSIDSPKLIVAVFVR
                     EEFEETLPGLSVIDGQMGGGGGQRYIGTPMQSPGGVSPAYGSSGGGYTYNFPNIPDDD
                     DDIPIPDDGGDDDIPIPGDGEVPEPATIFLLGLGTVALVISRKR"
     gene            complement(10746..11396)
                     /locus_tag="PLE88_04360"
     CDS             complement(10746..11396)
                     /locus_tag="PLE88_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75734.1"
                     /translation="MKKMWVMTMSCVLLGSAIQAGLAPTYLPSSSYYQGRHSFAYDLG
                     AQGILSGHLEFAVYKGTEAEVMMDWTGYQGQAQYVYAYQVFNDGANSNAALTYFALTG
                     INPGSIGSVTEDIGQAESVGSFDSEGIAPEGDGYFNASVTKAIWTFADGTLIKGTQSW
                     FLFLYSNYDWIKGDMEVQTTQNDDIPIPDDSNNNIPEPATMLLLVSGLVLSGLRKK"
     gene            complement(11438..11980)
                     /locus_tag="PLE88_04365"
     CDS             complement(11438..11980)
                     /locus_tag="PLE88_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75735.1"
                     /translation="MMKKLGMGLAFLAVVLLPAGAWATIIADAFFTYTPLSGPGTVTV
                     HSWVEDLAPTAALYRYNYQILDPSVNIQWFSAELLPNAAIVNWATYTGLPGEPALWAP
                     NGIGTSFDATFINPILAPGGKSAVLWFDSPQKPTTADGMAAGFTGGYKAFYGNLYTPV
                     PEPTTLLLLAAGAIGLLRKK"
     gene            complement(12680..13918)
                     /locus_tag="PLE88_04370"
     CDS             complement(12680..13918)
                     /locus_tag="PLE88_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOM75736.1"
                     /translation="MTPEFKIEVSSRIKRLPPYLFGRLNALKLEKRRQDIDIIDLGMG
                     NPMDGAPKMVIDKLCEAARDPRNHRYSVSKGIYNLRREMALKYERKWNVSLDPDSEVL
                     ACIGSKEGFSHMCLAMLGPGDTAIVADPAFPIHIYSVALAGANVITVPLGNDQKFLDT
                     IAYVCEHLYPKPKLLILNYPHNPTAMTVEPGFFDTVVDLARRFNIAVIHDFAYGETAF
                     DGYKPPSFLSAKGAKEVGMEFTTMSKPYGMAGFRIGFAAGNKAMLDALATVKGYYDYG
                     IFQPIQIASIIAMRHCEDAIVQQNAKYQARRDVVCDGLKRIGWDVEVPRASMFVWTRF
                     PKEHSKGKGSVDFAMELLENANVVISPGRAFGENGEYHMRIALVENEHRLRQAIRNIG
                     RYIQGKEVVGHRRKKSKDLT"
     gene            complement(14037..15050)
                     /locus_tag="PLE88_04375"
     CDS             complement(14037..15050)
                     /locus_tag="PLE88_04375"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HOM75737.1"
                     /translation="MGIEESSARGTSLMTNTFSFYQSLFFCLLILLPSRWGLAAEAVS
                     FYMDNDSKRLKPNHNTDRHYTAGTKLVFVTQPDWLWLQDFAHWHFGQDGRPVDIVAGF
                     FIGQNIYTPDHADEPAKRRPEDMLFAGWLYSGMFIQRAAEDTLDHIEVNVGVIGPSSG
                     AEQAQRCIHRYFHSGEPVGWEKQITDEFASDFTYIRQQRLAACLPAYSATMDFIADYG
                     FTVGSVHRFVQAGLTCRYGFNMGKTFAPGRLDQPDGISLLRRAVEKSGYIYLRGAAKA
                     VEYNRFLTGLEPEPLVGQLQAGIVLRYKKLDIGYSQTFFTQEFEEQSGKDSVGSLTLS
                     WQF"
     gene            complement(15041..16582)
                     /gene="guaA"
                     /locus_tag="PLE88_04380"
     CDS             complement(15041..16582)
                     /gene="guaA"
                     /locus_tag="PLE88_04380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOM75738.1"
                     /translation="MTREVIAIIDFGSQYGQLIARRVREHKVYSQIYQPSVRIEQLAQ
                     SGLKGIILSGGPASVYAQNAPTCDKRIFELGVPVLGICYGMQIGCQILGAEVRPAASR
                     EYGRTSLEIADASDLFKGISRHTTVWMSHGDQVNQLTEDFVPLAATPTCPFAAVRHNS
                     GRFFGVQFHPEVTHTPRGSEILKNFLYDICGCAGDWQMSDFVEDTIARVRQQVAGGTV
                     ICGLSGGVDSAVTAALLHKAIGSQLICIFVDNGLLRKNERQGVESMFREHFHIDLHVV
                     DWKEKFLAKLAGVTDPQQKRKIIGAEFIEAFKSEAKKIKDARFLAQGTLYPDVIESGS
                     KDGNLAANIKLHHNVGGLPEQLGFELVEPLRDLFKDEVRLVGEYLGLPEEMVWRHPFP
                     GPGLAVRIIGEVTEAKLVILREADDILIEEIRAAGLYRQISQALAVLLPIGTVGVMGD
                     ERTYDNVIALRCVETTDFMTADFSHVPFEVLGRIAGRIINEVRGVNRVVYDVSSKPPA
                     TIEWE"
     gene            complement(16599..18071)
                     /gene="guaB"
                     /locus_tag="PLE88_04385"
     CDS             complement(16599..18071)
                     /gene="guaB"
                     /locus_tag="PLE88_04385"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOM75739.1"
                     /translation="MNAEKIVAEGITFDDVLLIPARSDFVPSQADTATRLTRNIRINI
                     PIVSAAMDTVTESALAIALAQEGGIGIIHKNLSIEAQTREVTKVKRSENGVILDPVTL
                     TPAMTVASARQLMAEQNVSGIPIVENGKLVGILTRRDLKFLADDAVQIREVMTSKNLV
                     TGPADTTLDQAREILKKHKVEKLLLVKNNGELAGLITMRDIDRVQQFPYAVRDSRGRL
                     RVGAAVGVRDHDRIEALIAAEVDVIVVDTAHGHSKNVIEMVKWIKSNYAIDVIAGNIA
                     TAEAARDLIDAGADAVKVGIGPGAICTTRVISGVGVPQITAVMLAASEAEKSGVPVIA
                     DGGIRHSGDITKAIAAGASSVMLGSLFAGLAESPGQLVIYKGRQFKEYRGMGSIGAMV
                     QGSADRYGQDKGTEKDKLVPEGVEGRVPYRGTLSNFVYQLVGGLRAGMGYCGTPTIED
                     LRKRGRFVRISAAGVSESHPHDIMITKESPNYSAYEPFGK"
     gene            complement(18297..19034)
                     /locus_tag="PLE88_04390"
     CDS             complement(18297..19034)
                     /locus_tag="PLE88_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOM75740.1"
                     /translation="MGNSAAAVWQAGLHLLWPDKCLVCGQSIHPADEQLCRSCWQDLS
                     EAVSGDYCRRCGHDVSPYGIVGGRCGYCQQLELPYDGIVRVGRYESALRALILLLKFS
                     EQTQLADRLSQMLQQALLTSPFLSRIDMLVPVPLHWRRRLQRGFNQSCLLAKKLQSLN
                     IPTAADLIRVRNTEQQWDLTPAQRRRNVRGAFAVRRGHPFANKTIVLVDDVTTSGATL
                     EECARTLKLAGASGVYAAVVAAAHPVS"
     gene            complement(19099..19995)
                     /locus_tag="PLE88_04395"
     CDS             complement(19099..19995)
                     /locus_tag="PLE88_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75741.1"
                     /translation="MTDRKGFTLIELLTVIGITALLLAVAAPSLLLAKEQARGIYCRS
                     NLKQMCAAAAAYAMNSDDYYPIAYYQRFSAGQDGSALLPQEPPGDCGSQALSEAISFS
                     FCWDFTAVITADGRRLIRPGLLWQSDTLEKVHQCPSYKGSDNWSGNPYTGYNYNTSYI
                     GHGQGETVNPAVFTGIVKSAQDASGQSIVMPARTSHVRNPSSCVIFGEGHYAGGANKL
                     MRSPFVWEGDTDWDVRTGGTQGYRHMNQTNIGWAAGHVTAQRELYTDTHPRYKSQLEA
                     FNCSNKIKIGFLSPDNSLYDLK"
     gene            complement(20000..20926)
                     /locus_tag="PLE88_04400"
     CDS             complement(20000..20926)
                     /locus_tag="PLE88_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75742.1"
                     /translation="MAVCQVPATSDFAVEVTAAEGSFNGSRYRNPNAVLGKPAVQCLN
                     DRLWDPTLPVSFRVKLVEAAYNLSLDGAGVITTINPGESITVKFDHPVVDYPQNPFGQ
                     DFIVFGNAFFQYSQQVKISDQTNLNTVLLANPAQTAFGQIIVSVSQDGQIWHTFENGP
                     WADDLFPTQAYQWDRANARWTDIEMDFTRPVDPNLTLADFSGLTAAEAIDLYAGSAGG
                     TSFDLNDLPHYDQLLPDPNSGYRWIQYVRLEGGQGPFALGGQVDAVADVAACGDPTHP
                     YPSGDITRDCRVDMADLAIVAASWLECTYKCE"
     gene            complement(21025..21687)
                     /locus_tag="PLE88_04405"
     CDS             complement(21025..21687)
                     /locus_tag="PLE88_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM75743.1"
                     /translation="MKRFVVYMAGLTIAAAGFAGMTFEDIDFWVGQGTQRAALVIDWN
                     DGPNELVKVWGFRWDGTATGADLVLAVCAADADFYAMGGSGPYGFAIGGLGYDKNGNG
                     FFDISKTASASNFDSSGYMAVAGYAFDGWSAAESGDVWCGGWETDGFWGYFYNEAGVW
                     ISSMIGASSRVLADGDWDGWSWGAAQNGWYGGAPSASVAVPEPTAAVLLAIGLLFVSN
                     RK"
     regulatory      complement(22024..22237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            22718..24700
                     /gene="acs"
                     /locus_tag="PLE88_04410"
     CDS             22718..24700
                     /gene="acs"
                     /locus_tag="PLE88_04410"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOM75744.1"
                     /translation="MTEAKKGMESLLTEQRKFPPPPHIQSKAWISSMEEYQKMWEQSI
                     HNPDEFWLEQAKMLTWFKEPTKALEYTWNSKNRIIRHTWFADGQLNISYNCLDRHLTT
                     ATANKIAILWQGEEDKAVRKLTYAELHKEVCKFANVLKSKGIQKGDRVAIYMPMVPEL
                     AIAMLACCRIGAIHSVIFGGFSADAIKDRVNDSDCKMLITANVSLRAGKHIHLKNIAD
                     EALAKCPTIQNCIVVEVTNEPYEMKPDRDHWYHDEMAKVSSNCPAENMDAEDPLFILY
                     TSGSTGKPKGVVHTTAGYLLHAAMSHKYIFDIHDNDIYWCTADIGWVTGHSYIVYGPL
                     ANGATSLMFEGVPTYPDAGRFWQICDKFGVTVFYTAPTAIRALMRLGDEWVNKYKLDT
                     LRILGSVGEPINPEAWIWYYEKVGHSRCPIVDTWWQTETGGIMITPLPGAMTLKPGSA
                     TRPFFGVDPVVLRDDGTPCNRNEGGKLCIRKPWPGMMRTMWGAHDRFIDTYFSMFENV
                     YFTGDGCRVDEDGDYWLMGRIDDVVNVSGHRIGTAEVESALVSHPKVAEAAVTPIPHE
                     IKGQGLYAYVTLKEGVQESEDLKKELILHVRKEIGAIAAPEAIQFAPALPKTRSGKIM
                     RRILRKIAEKDTSNLGDITTLADPSVVEKLIAGRGK"
     gene            24785..26044
                     /locus_tag="PLE88_04415"
     CDS             24785..26044
                     /locus_tag="PLE88_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOM75745.1"
                     /translation="MNEQNPQYSIKNLGWRVTLAGMGINLALGILYTWSVFSKAIPAE
                     WNWTETHKSLPYMFACLIFSLVMVPAGRMQDKLSPKLAAAIGGILVGVGFIVASRTTS
                     PLGYLIGFGVLAGAGIGFGYASATPPAVKWFPKAKTGMIAGIVVSGFGLASVYTAPLS
                     KWLISEYTIQTAMLILGIAFFVIVVVLAQMLTPPPAGYSAEKNSSSSGVSADPTKKEH
                     FTPGEMLSTAQFYMMWFMYACGAGAGLMVIAKLAAIAEIQAGIKLGFLLVAALAIGNG
                     AGRILLGMLSDKIGRKATLFLCFVIQAAAIMLLSVTKQGTFLASVPILTVLSALIGAN
                     YGANLALFPSLTKDYYGLKNFGINYGLVFTAWGVGGFMLSYLAGRMYDIYKTFAIAYY
                     GASILLILAAACVFFIKPPHRQISNPQ"
     gene            complement(26109..26711)
                     /locus_tag="PLE88_04420"
     CDS             complement(26109..26711)
                     /locus_tag="PLE88_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM75746.1"
                     /translation="MFKPREIAVVLCAAVICGCAGQHGQYNRKTAKPVMEDPFNPDRP
                     PTAKTLYLMADILSSQGRDASAEQLFKRIQREYPDFLPAYNDLAAMQMRQRRLAEAME
                     TLSAGLKISPRDPVLLNNMGMCWLIRKDYAKALDYFTEAAAVMPTNTRYRANMAAALS
                     LLGRREEALALFKQILPEEEALENIRILCDRAASRPAASQ"
     gene            complement(26729..27241)
                     /locus_tag="PLE88_04425"
     CDS             complement(26729..27241)
                     /locus_tag="PLE88_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75747.1"
                     /translation="MKCGKTMLLLGVVCGMILNGCGEEKIIGPAPIDQNTVNQKLTAS
                     YSDLAIQNAIIAQHTLFPYHFVNNSAQLNALGERDLAVLAAHFRQNPGKIVLVQGQTE
                     PLLYQSRAQTVYEKLLQAGIPNEKIQIGSGFAGGEGIPSTAVIEILENAQTTTFKDTA
                     STGMTELRMQ"
     gene            complement(27269..28201)
                     /locus_tag="PLE88_04430"
     CDS             complement(27269..28201)
                     /locus_tag="PLE88_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM75748.1"
                     /translation="MMEFLHSESAVIIGILIFCAVFSIGLSILLYMAHLQKKKSVEIR
                     MLGVFEDDPFAEKPPKRSLSSFLVKLGNIASHGRASSSLWEQMIQAGYLNPYAPTVYT
                     GIKVLLFVIGFFITALLLLPFDSETLMLSSRLTLIFVGATAAFFLPNLYILYRMKHRR
                     DEIYRHLPEAVDLLEICVSSGLGLDMAWNIVADEIRQVSSVLSGAMSLANFEMHLGVS
                     RIEAMKHMAERTGVDELKSLAAILIQTDRFGTSIAETLQVFAQSIREERNFAAQELAE
                     KMSVKMLFPLILFIFPAVLIVMVGPAILALKTAF"
     gene            complement(28225..29205)
                     /locus_tag="PLE88_04435"
     CDS             complement(28225..29205)
                     /locus_tag="PLE88_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM75749.1"
                     /translation="MDPKTINLIISVTVFGLVLSVWCIGVLLWLGRYLLKLKAVQRRL
                     GISKAGQEDSQTLRLWRDLQIRQMPQETVEKDSIGLGKKLEYWVRDAGWKVPFRTVAL
                     GVVGLAALVFVMVYNIFGGIWLAVCGVFITLYLFVQYAQKRIMHRANLFERQLVDALG
                     VAARALRAGHPLVGAFQLIGEEIGDPLGGLFRQIYQEQAFGSDLKDSLKKAAREVRNT
                     EFKLFSTSVIVQLQSGGNLADLMDTLASVIRSRIWLNKRIRVLTAQTQFSKVILIAMP
                     ILMFFLLNAVDPEYMKPLYTTAGGNVLLILMIVNLLLGSWVMNKMAKIDF"
     gene            complement(29215..30561)
                     /locus_tag="PLE88_04440"
     CDS             complement(29215..30561)
                     /locus_tag="PLE88_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOM75750.1"
                     /translation="MNTGTDAGMNGFDSDKKIALHRIKSRVHQRLLGLLDLKEAQRFP
                     IEQLQKECTAKIDALLMQEKYPLTGPEKQQLLDEILDEIFGLGPLEPLLRDPTISDIL
                     VNGYQQVYIERRGRLERTDITFQDDAHLMRVIQRIGTNVGRRIDESSPMLDARLIDGS
                     RVNAIIPPLALDGPALSIRRFGTNPIDIHTLLELGTLTEEMSFFLESCVQCKINILIS
                     GGTGTGKTTLLNALSRWIPAGERVVTIEDAAELQLQREHVVRLETRPPNIEGKGQITQ
                     RDLVRNSLRMRPDRIIIGEVRGDEALDMLQAMNTGHEGSMTTIHANSPRDALRRLENM
                     VSMAGMNFPVRAIREQISSALDILIQIGRLVGGARKIINIAEITGMEGETVCLNDIFR
                     FQQQGVDENGNAFGRFESCGVRPRLVDKLIAQGIELPPTLFQRRVLETASAKKDVH"
     gene            complement(30585..31730)
                     /locus_tag="PLE88_04445"
     CDS             complement(30585..31730)
                     /locus_tag="PLE88_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM75751.1"
                     /translation="MTPKTKIFLVTKDPKTVSHIETVARNGSPIEIDKVFPEISHVPA
                     YLDEGEPAAGIVDIDPAPQETLSELEELIRNYPKMCTVVISEQITQELILSAMQAGAR
                     HFLQKGSIHSELSGVMARLLTHTGAKRKTGSIMTVFSASGGCGATTIALNLAMEMHVS
                     TSKPVLVIDLDRCYGAAAAYLDVKSEYGIDDVLTYEGRIDKHLIQSSSCCYKENFHVL
                     LKGSGASRKTGIDIKPESLRMVLQACREAYAYTVIDAPRMDEQTVRELSEQSDFTIVV
                     FQLTLKDLAFARSIISMFRTLGLPDEKIIPLANRFAKRGPLLRIDDGKRALGTDTMYL
                     VRSDWRNAMRCLNKGQTLAEGAPRSAIRKDLRKLAAVIQEVKSEEKR"
     gene            complement(31821..33296)
                     /locus_tag="PLE88_04450"
     CDS             complement(31821..33296)
                     /locus_tag="PLE88_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HOM75752.1"
                     /translation="MRTTASQSGVQMNSWIAAMVLAILASPGMAAIAADAAGSEVAIV
                     YHQSQIMQTPWKVSRVAVTDPAIADVQVLTANQVLIQGLAVGTTDILLWSEDETQVVQ
                     KKVQVTLDMDTLKADIGQLFPTSSLMLSQSGSNLVIRGQHKNALHAQQLQEYLSKMKI
                     PFIDMSEVVGVQQVELQVRVAEVSKTGLRSLGVDWTQAGSDFSSGVAPGGPIAESVSF
                     WPGAINAYEVGTPATAFGHITSADLVFFLDALQENQYLRILANPTLIALNGQEASFLA
                     GGEYPIPVPQSGTGTAATITIEYKEYGVRLNFKPVVLGDNTIRLYTAPEVSELDYTNG
                     TSINGTVVPGVLSRKTETTVELKSGQSFAIAGLLKNTISAANSAIPGLGNLPVLGPLF
                     RSTRYQQGETELVILVTANLVSPLDIDPKTSPLPGFLHKAPSDWELYLEGRIESRQPA
                     GLDGVDADWLKRLGLDQVSGPGAWDSYQSPAPLSRAQLQAE"
     gene            complement(33371..34246)
                     /gene="cpaB"
                     /locus_tag="PLE88_04455"
     CDS             complement(33371..34246)
                     /gene="cpaB"
                     /locus_tag="PLE88_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOM75753.1"
                     /translation="MKWTIVLLVVLGIIAGVCAVVLVSALQGSATIKEDVEVVVAKDN
                     LSAWKVLTVEDVEVIKVPRKEAPADAYSNTALVVGKVLTSDVVIRQAITKSKILTQGG
                     PQEIAAILAPRMRAVSVTVSSDQVSGGLLYPQCFVDVLATFKLSGSMRSESKGEAVAT
                     TLLERVKVLAIAGESILSAASKEDKTAASARTSQKLTVTLELDSKQAEALQLAINNGS
                     ISLVLRNPLDMEAVDPQPMVLNSGQLSGPGRPVIPVVGSNPEEGSKTTQRSTAITVIR
                     GNKTSEVQVPEESSQ"
     gene            complement(34310..>34705)
                     /locus_tag="PLE88_04460"
     CDS             complement(34310..>34705)
                     /locus_tag="PLE88_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75754.1"
                     /translation="IIIGHQRLVPLIYEKSIILTFGALKYGWLFFKWQQITNITRHAA
                     RIAILPGNRSGEVAALISTRLAECGIPANGVSISVPNGWNPGVGNELTLEISVPAANV
                     DILRLKGPLIGLPTPTSLTARVVMSKEGP"
BASE COUNT         8746 a         9358 c         8395 g         8206 t
ORIGIN      
        1 caaaatccat gtacatagaa cggggctttc tgcactgtcg ggagttatta tgttttttta
       61 cccggcagct tcagttttcc cgcaggtccg gcagggtctg taaattcaga aattctggat
      121 ttattttgat gacaatccag aatttctgaa attgatgatc tacaccctca taccccgtaa
      181 acatcgtccg gcagcggcaa tccgcactgc ctatccggcc ggcaattcct gcataacggc
      241 atccggtata ctgttccaaa ggcggcggtg tgcctgttga ggccgtcaaa taccatcgac
      301 aacaccggac aattagagtg ttatccagta ttgtcgacga caacactgat tttagtgtaa
      361 aaatccagag ctgttgataa ttgttgatta ctctttcata ctctatctgg cgtatgctct
      421 cactgccttc cagagctccc atctcctccg gcagcaacat ttgtcgccga tgccgctggg
      481 cttgctctgg atattcagca tccgctttgc cgtttgcaat gtgcagataa aaacatttca
      541 gctgccttgc agggcaggct gcagtgtttg agtgtctgta acaattgagg agtctataat
      601 tttcttgcaa gagttcttta ttttttgtaa agtcatcgaa aaattattga cctgttatgg
      661 tttgacagcc gctttctggc atggcagctt gccagccgga cagcaggaga gttagtgtta
      721 ttataattgc agaagatgct ggaagaacac cttcccatcg ctgtataatc attttctaag
      781 taagcagtta cgccaacgta gctcaacggt agagcaccgg ttttgtaaac cggcggttaa
      841 gggttcaaat cccttcgttg gcttttttgt atttttggga taaaaccttg acagttgagg
      901 atatttgatg gaacaggcga ttgaaaaggc cggtgcttta atagaagcga tggaatacat
      961 ccgccggttc agggacaaaa tcgtagtcgt aaaactgggc ggcagcattc tggacgactg
     1021 tgaacttcag aaaaatcttc tgaccgacat tgtgtttatg tcgatcgtcg ggatgcgtcc
     1081 aattctggtg cacgggggcg gcaaagccat taccgcagcg atgaatgagg cgggactgga
     1141 accggtatgg gttcacgggc ggcggtatac tgacgaacgc acattgacca tcgccgagca
     1201 taccttaatc aaaaccgtca atcagcccat ctgccagacg ctgcaggaaa tgggcatcaa
     1261 ggccatgggg ctgcattcat tgagcagctg cgttgtattt gctgagccgc tgcgtttgga
     1321 aaacggcgac ggtcggaaaa ttgatctggg ctttgtgggc aaggtttcgg aggtcaatgc
     1381 cgaactgctg attacactgt gcggcagcgg agtgattccg gtgattgccc ccgtcgcccg
     1441 cgccaaaacc ggcggcaagc tcaatgtcaa cgctgacagt gtagccggcc acatagcagc
     1501 tgcggtaaaa gccgaaaagc tcgttgttct cagcgacacc cacggcatcc gccgggatgt
     1561 cagcgatccg ggcagctgta tttcaagcat cagtgaaaag gaaattgccg gcatgatagc
     1621 ggacaacgtt atctcgtcgg gaatgattcc caaagtagag gcctgcctga cagcgctgga
     1681 cggaggcgtc ggcaaggccc atattatcga cgggcgcatc ccgcattcgc tgctgctgga
     1741 aatttatacc gataaaggca tcggaacaca aattgtcaaa taggccgtca caataaggac
     1801 acctgcaatg aagactcaag atataatcga aatgtacagt cgttatgtaa tcggcaatta
     1861 tggccgcctg ccgcgggtta ttgtcaaggg acagggcaac aaactctggg accttgaggg
     1921 caatgaaatt ctggactttt tccccggctg ggctgtcagc ggcatcggcc actgccatcc
     1981 caaagtcgta gaggccattc gccggcaggc gggggaactg ctgcatatgg acaacacctt
     2041 ttatacgctc cagcagggcc agctggccag actgctgtcc gagcggggct tcggcggcaa
     2101 atgctttttc tgcaacagcg gtgctgaggc caacgaagca gccattaagc tggcccggcg
     2161 gcatacgcct cgggaaaaat acaaattcat caccgccgaa aagagttttc atggacgaac
     2221 gctggcgact gtgaccgcta ccggacagcc caaataccac gagggattcc tgccgctgcc
     2281 gcccggattt gtgtatgtgc cctataacga catcgatgcc ctgcatcagg cgttcaatga
     2341 ggaagtggcc gcagtgatga ttgagccgat tcagggggaa ggcggcatcc atgaggcaac
     2401 gcccgaatat atgcagacga ttcgggagct gtgcgatgca cacggcgcct tgatgattct
     2461 ggatgaagta cagacaggtc ttggacgcac cggacggtgg tttgcctacc agcattatga
     2521 ggtcacgccg gatattatca cgatggccaa ggccttgggc ggcggggctg ccatcggggc
     2581 gatgatggca aagccgcaaa tcgccgcctc gctggttccc ggcacccacg cctccacctt
     2641 cggcggcaat ccgctggcct gcgccgccgg cattgcggtc attgaagcca tcgagcaaga
     2701 acatctgctg gacaatgccc acacgatggg cgattatctg cggacccggc tggaacagtt
     2761 aagggaaaaa tacccgatta tcgaccatat ccggggcaag ggtctgatga ttggcgttca
     2821 gctgaccatg cccggagcca aaattgtcag ccggtgtctg gaaaagggcc tgcggatcaa
     2881 ctgcacgcag gagactgttc tgcggctgat gccgtccatg acgattacgc agccggagat
     2941 cgacaaggca ataaccatac tggatgaggt tttagcagag ggacagcaag catgaaaagc
     3001 tttttatcca ttgccgattg ttcgcgggac gaactggaag cactgctgac gctcagcagc
     3061 gaactcaagc gtctgtacaa aagcggcggg cgcgatctgt gtctgtccgg caaggtgctg
     3121 gcgatgctgt ttgaaaaaac cagcctgcgg acgcgcatca gctttcaggt ggcgatgagc
     3181 gatttgggcg gcacagccat ttatctcaag cccgaggaca tcggcatcat cggcaagcgc
     3241 gaaccggcca aagatatggc ccgcgtgttt tcgcggtatg tggacggcat tatggcaagg
     3301 acctttgccc atgagacgct gctggagctg gaccggtggg cgacggtgcc ggtcatcaat
     3361 gcattgagcg actggtcgca tccctgtcag gcgatggcgg acatgctgac cattcaggag
     3421 cattgcggca agctggcggg gctgaagctg gcgtatatcg gcgacggcaa caatgtcgcc
     3481 cggtcgctgg catttgcctg tgccaaactg gggatggcct ttgtgtgtgc atcgccggca
     3541 ggctatgagc tggacaaaca gacattggag gcagccaacg ccgtgactgc cggctgtgcc
     3601 gttcagatac gctgtcctga agaagcagtc cgcgatgccg atatagtcta tacggacacg
     3661 tggatcagca tggggcagga agaggaaaag gcccagcgga tacgggattt tcagggctat
     3721 gcggtggata ggacactgct gtcgaaggcc gccaaaaagg tcaaggttat gcactgcctg
     3781 ccggcttacc gcggcaatga gattaccgat gaggtaatgg agtcggaaca gtcaatcgtc
     3841 tttgatcagg ccgaaaaccg cctccacttc cagcgtgccc tcctgaaata cctgatggaa
     3901 taaggcaagt ttgatgaaaa ttcttgttgc gacaaccaat cccggcaagc tggaggaatt
     3961 gtcgatgctg ctgggagata ttgcaggcag gattgaatgg gtctcgctgc gggattttcc
     4021 cggtctgccc gaaatcgtcg aggacggggc gacctttgcc gacaacgccc gcaaaaaagc
     4081 cctcggctat gcccaagcga caggcctgtg gactttggca gatgattccg gcttgtgcat
     4141 cgatgccctc cacggtcagc ccggtgttct cagtgcccgc tttgccgccg atgaatgccc
     4201 cgctgctgac cgcaaaacgc tggataaggc caattaccaa aaagtcctgc gcctgttaaa
     4261 agatataccg gattcgcagc gtaccgcccg gttcttctgc cacttggcgc tggccgacga
     4321 caagcaaatc ctgctggagg caacgggcgc cgtcgaaggc gtcattatta accagccccg
     4381 ggggcaaaac ggattcggct atgacccgat tttttatatt ccgtcgctgg gcaaaaccgc
     4441 cgcacagctt gacaacagca gcaaaaacca agtctcccac cgcggccatg ccatccggca
     4501 gatgaaaccg cttctggagc agcttttggc acagcgtcaa gccggccgct aaaacgtata
     4561 tgtcatcgtg accgccactg ccggggcgtg cgaacggaag gcgcccgagg aaccgtagtt
     4621 gcgtttattc aaatccttgt caaaaatacc cagcagattc atgccgtcca cacggaaggt
     4681 caggttttta tccggctgat actgaagacc caaattgagg tagtaattgc ctcggtagcc
     4741 gcgctcccag tccggatcga tcggatggtt cgggttctgg cttaggcggt atcgttccag
     4801 gtcctttatc ccgctgaatc cccaataggt gcgcagcgaa ccgtcgacgg ttaactggtc
     4861 tgtcagcttg tgacacgccg ttaatttggt aacatgtttg gaccacaggg ccgagtcctc
     4921 tccggcgccg tacgcttcag acgtgagggt ggttgattgc ccctctgcca gatcaaattc
     4981 atagagcttg gtaaagccgt gtgaaatgcc gaaccgcgtt ttgtcggtat gataatacgc
     5041 ctccagctcg ataccatagt tcttttgcgt tccgatggga actggcgcac cggtggatcc
     5101 ggaccaggag atcagttcga ggttgtagtg ccagaagaac gaagcggcca gatcaatatt
     5161 cttcgtgtgc gtacgctcat aacgcaattc gacgctgtcg agtttttccg ggtcgccgtc
     5221 gcccagaccg ttcagcgctc cggcccgcat ttcttcctcg aaattagccc gaacggatcg
     5281 tgcccacatg agtttatagg tgtccttttc gttgggcgta tggaccagcg cggcgcgcgg
     5341 ggaaaacatc ttgccgctgt aagtatggtc atcgacgcgg gcgccgagaa acgtcgtcca
     5401 ttggtcggta atgttccact ggtattcgcc aaaaagcgag tacaggttgg tggaccaccg
     5461 gcttgcattg tcgccgctga gcatatcgtt gctgcttttg aatcctatat ccggccagcc
     5521 gtgcggatcc agccccagtt cgtgatgtga gacttccgca ccgaacgcgg cctgatggcg
     5581 ttcattaaac cgatgccgga gcaatacccg cgcatagtac tcgtcttcac gatacgagtc
     5641 ggcgatactg ttgatgacaa accgattata atcgaacata tcatagccga agcccaaact
     5701 taaatcggtt ttctccgata agctggtgtc ataccccgcg taacctgtta attgctgata
     5761 ttgataaaaa ttctgcgcca ggggatacgt gtcccaggca ggaccccact ggccgggctc
     5821 atactcaacc caatccacga tgccgccgcg cagccagtcc gtccagccgt agggaactcg
     5881 tgccagggtc gagccctccc atgaaaacgc ctgcccgccg cgcgtgtacc gtgcccaata
     5941 ggtccagtca cctcgattga tctgcccgaa caacttgacc ggcggaagac cacgggccgc
     6001 cgcctggtcc cggggcatgt tgatactggt catcgcgtca cctgcggctg tgccgtcgcc
     6061 gggatagtag tccgcgccgg tttgaccggt ccagtcaaac catgacacgc tcggaaaatc
     6121 aaatccgtag tggaccggcg catcatactt gctggcgccc acataattgc cgatgccggc
     6181 gtatccaaaa ataccgccgt cattatccga aaactcgcgt ccgtgtcgaa tttccaccga
     6241 ttcgaattct tcaaccacac ctgcccgtgt cgtgatttct gttccctgga atgtcttata
     6301 gttatgcgtg acaatattga tcaccatgga taccgcaccc ggtccataca acgccgatcc
     6361 cggcccgcgc accacgtcaa tgtgatggat atcccgcagc agcaccagat cgcgctcgct
     6421 gatggcgccg taatgcgtgc ggtcgttcat tactttgccg ttgaccgtca gcaggtactt
     6481 atcgtcgcgg tcgctgataa tgccgcgcaa accgatatca tccggttccc accagtttcg
     6541 aatccactgc aggttcggca cataaatatc cagcagttca taaagcgagc gggcacccga
     6601 ggcctgaatg tccgcctcgg ttatggttgt gatagcagac ggttgcagtc gccgtgtcgc
     6661 ggaggtcagt gaagccattg aagtgatttc cacattcatc agctcctcca gcgacagttc
     6721 gaataaattc tccggcttgg cagcttcctg tgcagcgaga gtggaactta tgaaagcggc
     6781 aagaatgaaa agaccccccc aaagctttct gtgcagtcgc aattccatct tttagggcct
     6841 ttcaatgttt tactcttttg tctcgaaatg ccgatgcaat cggtaattat agcttcggaa
     6901 gtcggaactt tacctgccgg atttaagtat tttttcgaga tgtccgataa aaagatgact
     6961 taagtgatat taagtgtacc aatcatccaa ataaaatttt ttaagatatt tatttaccgg
     7021 actttccaga ataaatttat ctatttcatc agcaaaacag atttatttga ttggcgtaga
     7081 aagagaacag acaggttttt taattttctt gacaggcaga caaaggattt gttatctatc
     7141 gactaaccac agattgataa cggtaaaacc ggagcagata ttattatttt acttaaccga
     7201 taaggcattt tatggcaaaa accggaaaat cccttgtaat cgtcgaatcg ccggcgaagg
     7261 ccaagacgat aaacaaatat ttaggcagca gcttcgaggt caaagcgtcg atgggacacg
     7321 tgcgcgacct tcctgccaaa ggtctcaatg tggacattga gaacaatttc gaaccgacgt
     7381 atgaaataac gccgggccgc aaacgcacgg ttcaggcctt gaaaaccgcc gccaaaggat
     7441 gctccagcgt ttatctggca accgaccttg accgtgaagg ggaagccatt gcatggcatt
     7501 tgaaagaatg tctcggcctg tccgacgaga atacctttcg tgtggttttt aatgccatca
     7561 ccaaaaacga cattcaaaag gcctttgcaa atcccggcaa aatcgatatg gaccgtgtta
     7621 tggcccagca ggcccggcgg atacttgacc gaatcgttgg ctatcaaatc agcccgctgc
     7681 tgtggaaaaa ggtaacacgc ggccttagtg ccggccgcgt tcagtctgtt gctgtcaaaa
     7741 tgattgtcca gcgggaacgg caaattcgtg cctttgtgcc ggaggaatac tggctgattc
     7801 ccggaatctt taccaccagc agcagctgca gcctcggcgc ggactggaaa gcatttatgg
     7861 aatcggcagc ggacaaagaa aaaggacgct gcctgtctga acagcagcag tggctggcag
     7921 cgcacaatgc attcaaggcc gatctaatta cggtcaatgg agagaaattt caggccaaaa
     7981 ttgctcagca ggcagaagca cttgtcagtg cgctgcggga atcccactat actgtaacgg
     8041 ataaaacaac ccggcggatt cagtcccggc cgtcaccgcc gtttattacc tcgacactcc
     8101 agcaagctgc cgccaaccgg ctgggttttg cgaccaagcg caccatgctg gttgctcagc
     8161 agctctatga aggcatcgac ctcggctcca tgggatcatt gggattgatt acctatatgc
     8221 gtacggacag cacgcatttg tcttctgaag ctatcagcgc tgttcggcga tacatcggct
     8281 cgcaaatcgg ccagcagtat cttcccgaaa aaccgaattt ctacgcctca agcaaagatg
     8341 ctcaagaagc acacgaagcg attcgcccca ctgaccccga cctgattccc agcgaaatca
     8401 agccgttttt aacagatgac caatacaaat tatacgacct gatatggcgg cggtttgtgg
     8461 cctgtcagat ggctgctgcc cagtgggata ccacacatgt ggaaatgaca ggtcaaaagg
     8521 atggattttc ctgtttatac cgtgccaccg gccgcgtgct ggtgtttgac ggctttacaa
     8581 aaatctggcc gacctccaca gaagagccgc agctgccggc tctggagaaa ggcgaccgtt
     8641 tgggcatcat ttcgatagat gccgagcagc attttacaaa accccctgcc cgctacacag
     8701 aggcctcgct ggtcaaagcc ctcgaaaaag aagggattgg ccggcccagc acttacgccc
     8761 agatcatttc cacgattcag gagcggaaat atgtcgagca aatcgacaaa aagttttatg
     8821 caaccgatct gggtgaaatc gtcaccgaca agctggaaga atgttttccg cgggtcatgg
     8881 atatcgcctt cacccgccat atggaagagc agcttgataa gattgaagag cagcatctta
     8941 actgggtgga tgtcctcaac gagttttaca ggccgtttaa ggacaatctc gaaaaggctt
     9001 ttacaacaga acacgccaaa gcccaaaccc agcccagcga ttatacctgc ccggactgca
     9061 aagccccgct ggtttaccgg ctgggcaaaa acgggcgatt tttaagctgt tcggcatatc
     9121 cgaactgcaa atttgcctgt ccctgcgaca aagacggcaa aatgctgcag gaaaagccca
     9181 gtgagcatct gtgtccggtc tgttcgaaac ccttggttca gaagcgggga cggtttgggc
     9241 catttttggg ctgttccgat tatcctgcct gcaaaaccat cgtgaaactg gataagcaag
     9301 gccagcccct gccgcccaaa ccgccggctc aatcgtccgg cgtcaaatgc tacaaatgca
     9361 aaaacggcga cctgcttatc cgccagagca agcgcggtcc ctttctggga tgcagcaatt
     9421 tcccccgctg ccggacaatg gtaagcatga agcatctgga cgagctgaaa aaactgcaag
     9481 cagagggcca atggccgccg gccagcgaag aagaagcccg ccgcatcctc ggaatagacg
     9541 ctgcagcgaa ggcaaaaact tccaaaaaat ctgcaacagc gaggcaagta tgacaaaatc
     9601 cgacaagaat atttcaatta ccaataagga aacatttgag acaatcgaac cgatcggcaa
     9661 gcgcgttttg atccgcaaag acgaagataa aaagcagacc aagggcggga ttcagcttcc
     9721 cgataatatt gaaatcccga cgatcaccgg acggattgtg tccgtttcgg cagaagtgga
     9781 gatgtccagt gaatttccgc tcaaacagta cgacaaggtc ttgttcaatc caaagggggc
     9841 aatccctgtg gatttcgagg gcgacaatcg gctgtttgtc gtcgaggttg aaaacatcgt
     9901 agcggttttt cggaaacagt agccgtgcgg atttaaaaaa atacagccga acgcacccgt
     9961 cgttcggcca tatcatctaa acctcctcca gcgccaatct ccgtttcttc cttggacgct
    10021 gcaatcaatg ctatgctatc agtgctgtcg gcgcctcagg aggaggtcgt tttctatcgc
    10081 ttgcggctta taacaagagc cactgtaccc agtccaagca gaaaaatggt agccggttca
    10141 ggtacttctc catcgcccgg aatcggaata tcatcatcgc cgccatcatc cggaatggga
    10201 atatcatcat catcatcggg aatattggga aagttgtagg tgtaaccgcc accggaagat
    10261 ccataggcag gactgacacc gccgggagac tgcataggag tgccgatata ccgttgaccg
    10321 cctccgccgc ccatctggcc gtcgataaca ctaagtcccg gaagcgtttc ttcaaattct
    10381 tcccgaacaa atacagccac tatcagtttg ggggaatcaa tcgatacaat cgcatcggca
    10441 ggaccattcg aggagacatc gcccagccag taagcaaaac gataaccccg attcggcgtc
    10501 gctgccagag gaattttctg ccccaccggc atcgccaaaa cacccatccc gggcgagaca
    10561 acgcccccgt ccggaggact gctttggatt aaaagcgtat agctgttttc gtcggctccc
    10621 cgtacaccag cagaaacagc cagaaaaacc agcacacacc ccgctatcac actccaccgt
    10681 ggattcattt tctttaggcc tttcataaaa ttgcgccccc cggcagcaat attgatgatg
    10741 ccgttttact ttttccgcaa cccggacaaa accagtccgc ttaccagcag cagcattgta
    10801 gccggctccg ggatattgtt gttgctatcg tctggaatcg ggatgtcgtc gttttgcgtg
    10861 gtctggacct ccatatcgcc cttaatccaa tcgtagttgc tgtataggaa gagaaaccat
    10921 gactgcgtcc cttttatcaa ggttccgtct gcaaatgtcc agatggcctt cgtaacggat
    10981 gcattgaaat agccatctcc ttccggagca atgccttcag aatcaaagga gccgacggac
    11041 tcagcctgtc cgatatcttc cgtgacagag ccgatagaac ccggattgat gcctgtcagg
    11101 gcgaaatagg tcagtgccgc attgctgttg gccccgtcat taaaaacctg gtaggcatac
    11161 acatactggg cttgtccctg atatccagtc cagtccatca tcacttcggc ctcggttcct
    11221 ttgtaaacgg caaactccaa atggccgctc aaaatgccct gagcccccaa atcataagca
    11281 aaactgtgcc ggccctgata ataagaactg gacggcagat acgttggtgc cagcccggcc
    11341 tgtatggccg aacccagcaa tacgcacgac attgtcatca cccacatctt cttcatcgca
    11401 cacaacctcc gatcttaaaa aattaaacca tcacccgcta tttttttcgc agcagtccta
    11461 ttgcccctgc tgccagcagc agcaatgtcg tcggttcagg aaccggggta taaagattgc
    11521 cgtagaacgc cttgtagccg cctgtaaatc ctgccgccat gccgtctgct gttgtcggtt
    11581 tctgcgggct gtcaaaccat aaaaccgcag atttcccgcc gggggccagg ataggattga
    11641 taaatgttgc gtcaaaactc gtaccgatgc cgttgggtgc ccacagagcc ggctcgccgg
    11701 gaagaccggt ataggtcgcc caattgacaa tcgccgcatt gggaaggagt tcagcagaga
    11761 accactgaat attgaccgat ggatccagaa tttgatagtt gtagcggtaa agggcggctg
    11821 tcggagccaa atcctctacc caactgtgca ctgttacagt ccccggaccc gacaaaggag
    11881 tgtaagtaaa aaaggcatct gcgataattg ttgcccaagc acctgccggc agcagaacaa
    11941 ctgccaaaaa cgccagcccc attcctaact tcttcatcat caatctcctt tcttacagca
    12001 aaagaaacaa tcctttggat aaagcccgtt atgaaaaaca aaaaggtgtt tcaggcttat
    12061 ggactgcttt gagcagcccc gcgctgaaca gcttttacga ttctcaaagg acgttcactt
    12121 caaaaagagc aattcatccg cctttcggct cgatcaattc ctctccccgc tgtccatgca
    12181 gtacataacc tgctctggaa cgacactctg tcctcatcct cgtaaacgtc tttccagctc
    12241 ttctcctgcc tgtatgaaat gaagcttttt gaactgaacg gtcaaaaaaa cttcctgttt
    12301 cacattgcga ttcctctttc ccgtcctcgg cgataaaaaa ctcttatcgc caacagaatc
    12361 tcaactcctc acagaaagtc tttccaacac tccccaaaaa atgaattgac agctcagcaa
    12421 gataaaaaac tcaaaacata ttcaacaact tgccaagctc gtatctttcc tacttccccc
    12481 actgtcgatg atgaccttat taaagcaaat attccacttc aagtcaagta aaaaacttga
    12541 agaaatccat tttttttatt gttttctatc cagctctgca aaagaagcat aacttattgg
    12601 aatctattga gttaagaaac aataaaaaac cctgaaaaag agcgtttcca gtactgaaaa
    12661 aacggtttta aaaattattt caggtcaaat cttttgactt ttttcggcga tggccaacaa
    12721 cctctttccc ctgaatataa cgtcctatat ttcgaatggc ctgacgcagg cgatgctcat
    12781 tctcgacaag agcaatccgc atatgatatt cgccgttctc accgaaagca cgtccggggg
    12841 aaatcaccac attcgcattc tcgagcaatt ccatcgcaaa atcgacactg cctttgcctt
    12901 tggaatgctc cttaggaaaa cgcgtccaga caaacatgga tgcacgcgga acttcgacat
    12961 cccagccaat ccgtttcagc ccatcgcaga caacatcccg ccgtgcctga tatttagcat
    13021 tctgttggac tattgcatct tcacaatgac gcattgcgat aatgctggca atctgaatcg
    13081 gctggaaaat accgtaatca taatagcctt tgactgtagc cagcgcatca agcattgcct
    13141 tattgcccgc tgcaaatccg atgcggaaac ccgccattcc atagggtttg ctcatcgtgg
    13201 taaattccat tcctacctct ttggctccct tagcagaaag aaaactcggc ggtttatacc
    13261 cgtcaaatgc cgtttcacca taggcaaaat cgtgaatcac cgcaatattg aaccgtctgg
    13321 ccaaatctac caccgtatca aaaaatccgg gctctactgt cattgcagtc ggattatggg
    13381 ggtaatttag aattaaaagc ttcggttttg gatataaatg ctcgcataca taggcaatcg
    13441 tatcgagaaa tttctggtcg ttgcccagcg gaacagtgat aacatttgca ccggccaaag
    13501 ctacgctgta gatatgaatt ggaaacgccg ggtcagctac aatcgccgta tcccccggtc
    13561 caagcatagc cagacacata tggctgaagc cctccttgga gccgatgcag gccaagacct
    13621 cactgtcggg atccagcgaa acattccatt ttcgctcata ctttaatgcc atttccctgc
    13681 ggaggttgta aatacctttg gacacgctgt aacggtggtt gcgagggtct ctggcagctt
    13741 cacacagctt gtcaataacc atcttggggg ctccatccat gggattaccc atccccaagt
    13801 ctataatatc tatatcctga cgccgctttt cgagttttag ggcattaagc cgcccaaaca
    13861 agtaaggcgg aaggcgttta atccgggacg atacttcaat tttaaattca ggagtcatag
    13921 tgtctcaatc tctaaaggtc gtaactgtct gcgaaattta gtgagaaaat ccgaaatttc
    13981 aacacaaatc ccgctttggg tattttaatt atatcggttc aaggcagcaa ataaaatcag
    14041 aattgccacg acagtgttag cgaaccaaca ctgtccttgc ccgactgttc ttcaaactct
    14101 tgggtaaaaa aggtttggga atatcctata tcaagctttt tataccgtaa aacaatgcct
    14161 gcctgcaatt gtcctacaag aggttccggc tcaagtccgg ttaaaaaacg gttatattca
    14221 accgctttgg cagccccgcg aagataaata tagccggatt tttctactgc tctccgcagc
    14281 agagaaatgc catcgggctg gtcgagtcgt cctggggcaa atgttttgcc catattaaat
    14341 ccatagcggc acgtcagccc ggcctgaaca aaacgatgca ctgaaccaac tgtaaatcca
    14401 taatcagcga taaaatccat cgtggcagaa taagccggaa gacatgcggc aagccgctgc
    14461 tgccgaatgt aggtgaaatc ggatgcaaat tcgtcggtta tttgtttttc ccagccaacc
    14521 ggttctcctg aatgaaaata acggtggata cagcgctgtg cctgttcggc cccagatgaa
    14581 ggaccaatta cacccacatt cacttcaata tggtccaatg tatcctctgc tgcccgctgg
    14641 ataaacatgc cgctgtagag ccagccggca aaaagcatat cctccggacg gcgttttgcc
    14701 ggttcatctg cgtggtctgg ggtataaata ttctgaccga taaaaaaacc cgctactata
    14761 tcaaccggcc ggccgtcctg cccaaaatgc cagtgcgcaa aatcctgcag ccacagccaa
    14821 tccggctgag tcacaaaaac cagcttggtg ccggctgtat aatgacggtc ggtattatga
    14881 ttgggcttga gccgcttgct gtcgttatcc atatagaatg aaacggcttc tgctgccaat
    14941 ccccaccgac tcggcaacaa aatcagaaga caaaaaaaca aactttggta aaatgagaat
    15001 gtattggtca tgagtgaagt accccgcgct gacgattcct ctattcccat tctattgtag
    15061 ccggcggctt gctggacaca tcatagacca cacggttgac gccgcgcacc tcgttgataa
    15121 tcctgccggc aatccgtcca agcacttcaa aaggcacatg agaaaaatcg gccgtcataa
    15181 agtctgtcgt ttcgacacac cgcagagcaa tgacattgtc ataggtgcgt tcgtcaccca
    15241 taacccctac cgtgccgatc ggcagaagca ccgccagagc ttggctgatt tggcgataca
    15301 gtccggccgc ccggatttcc tcaatcagaa tatcatcggc ctcccgcagg attaccagct
    15361 tggcttcggt aacctcaccg ataattctta cagccagacc ggggccgggg aacgggtgtc
    15421 tccaaaccat ttcttccggc agtcccaaat actcgccaac cagccggact tcatctttaa
    15481 ataaatcgcg cagcggctca accagctcaa agccgagctg ttcgggaagc ccgccgacat
    15541 tgtgatgcag cttgatattg gctgccagat tgccgtcctt gctgcctgat tcgatgacat
    15601 ccggatacag tgtgccctgc gctaaaaacc gggcatcttt tatctttttg gcttcggact
    15661 tgaaggcctc gatgaattcg gcgccgataa ttttgcgctt ctgctgcgga tcggtgacgc
    15721 cggccagttt tgccagaaac ttctctttcc agtcgacgac atgcaggtcg atatgaaaat
    15781 gctcccgaaa catcgattcc acgccctgcc gctcattttt ccgcaagagg ccgttatcca
    15841 caaagataca gatcaattgg ctgccgatgg ctttatgcag cagtgcggct gttaccgccg
    15901 aatccacacc gccggaaagt ccgcagataa ccgttcctcc ggcaacctgt tgtcgcaccc
    15961 gagcgattgt gtcctccaca aaatcgctca tctgccagtc acctgcacaa ccgcagatgt
    16021 catagagaaa gttcttgaga atttctgacc ctctgggcgt atgggtgact tccggatgga
    16081 actgaacccc gaaaaacctg ccgctgttgt gacgtaccgc cgcaaacgga cacgtcggcg
    16141 tggctgccag aggcacaaaa tcctccgtca gctgattaac ttgatcgccg tgactcatcc
    16201 agacagttgt atgacggctg atgcctttga acaagtctga cgcatcggca atttccagcg
    16261 aggtccggcc gtactcgcgg ctggcggcag gacgcacttc agcccccaga atctggcagc
    16321 caatctgcat cccataacag atccccaaaa ccggtactcc aagctcgaaa atccgtttgt
    16381 cgcaggttgg agcattttga gcatatacgc tggccggccc gccggacaga attattccct
    16441 tcagaccgga ctgcgccagc tgttcaatac gaaccgacgg ctgataaatc tgcgaataga
    16501 ccttatgttc gcggacccgc cgggctatca gctgcccgta ctgactgccg aaatcaataa
    16561 tggctattac ttcgcgtgtc atctgcttta tctgtgtttt actttccaaa tggttcgtag
    16621 gccgagtaat tcggcgattc cttggtaatc ataatatcat gggggtgaga ttcgctgacg
    16681 ccggcggcac tgatgcggac aaaccgcccg cgcttccgca gatcctcaat agtaggcgtt
    16741 ccgcaataac ccatccctgc ccgcaggccg ccaacaagct gatagacaaa attgctcagt
    16801 gttccgcgat acggcacacg gccttcgacg ccttccggca caagcttgtc cttttccgtc
    16861 cccttgtcct gcccatagcg gtcagctgag ccctgcacca tcgctccaat cgagcccatt
    16921 ccgcggtatt ccttgaactg cctgccctta taaataacca gctggccggg gctttcggcc
    16981 agcccggcaa acagcgaacc gagcatcacg cttgaggcgc cggcagcgat ggctttggtg
    17041 atatcgccgg aatggcgaat tccgccgtcg gcaatcactg gcacaccgga cttttcagct
    17101 tcagaagccg caagcattac tgccgtaatc tgcggcactc ccacgccgga gatgacgcgg
    17161 gttgtgcaga tagcccccgg gccgattccc accttaaccg catcggcacc ggcatcaatc
    17221 aaatcccgcg ccgcttcggc tgttgctatg ttgccggcaa tgacatcgat tgcatagttg
    17281 gatttgatcc atttgaccat ctcaatgaca tttttcgaat ggccatgagc ggtatcaaca
    17341 acgataacat ccacttcagc tgcaatcagt gcttcgatgc gatcatggtc acgaactccc
    17401 acagccgccc caacccgcag acggccccgg ctgtctctta ccgcgtatgg aaactgctgc
    17461 acacgatcaa tatcacgcat cgtgataaga cccgccaatt cgccgttgtt tttgaccagc
    17521 agcagttttt cgactttgtg ctttttaaga atctcgcgtg cttgatcgag tgttgtatcg
    17581 gctggtccgg tcaccagatt tttgctggtc atcacttcgc gtatctgcac ggcgtcatcg
    17641 gccagaaatt tcaagtcccg acgggtcaga ataccgacca gtttgccatt ttcgacaatt
    17701 ggaatacctg aaacgttctg ttcggccatc agctgccgcg ctgatgcgac cgtcatagct
    17761 ggcgtcagcg ttaccggatc cagaataacg ccgttttctg accgcttaac ttttgttacc
    17821 tcccgtgttt gggcctctat cgacagattc ttatgaataa ttccaatgcc cccttcttgt
    17881 gccaaggcga tggccagcgc tgattctgtt acagtatcca tcgctgctga aacaatcggg
    17941 atattgatgc ggatattgcg ggtcagacgc gtagctgtat cggcctgact cggaacaaaa
    18001 tcgctccggg ccggaatcag cagaacatcg tcaaatgtga tgccttcggc tacaattttc
    18061 tctgcattca tacaacggct cccgccttaa aaggtctttg gaattttcac tcattctgta
    18121 aggtggttag tgtacataac agcccgtttt tcgtcaaaga aattcccgac acaagcagcc
    18181 aaaatcccat acaagaccct ttatgtcttt ttggcagggt ttacaggata ttggagagca
    18241 atccgatagt taatcgaatt gagcagccgt ccatcggcaa ggcatctttc accagtttat
    18301 gacacaggat gggcggcagc cacaacggca gcatacaccc ctgatgctcc ggccagctta
    18361 agggttctgg cgcattcttc aagagtggct ccgctggtgg taacatcatc cacaaggaca
    18421 attgtcttgt ttgcaaatgg atgccccctg cggacagcaa atgcaccgcg gacattcctc
    18481 cgccgctggg caggcgtcag gtcccattgc tgttcggtat tgcggacacg tatcagatcg
    18541 gcggctgttg ggatatttaa cgactgcaat ttctttgcca gaagacaaga ctggttaaaa
    18601 cctctctgca gacgcctgcg ccaatgcaga ggcacgggaa ccagcatatc aatgcgggac
    18661 agaaagggac tggtcagcag agcctgctga agcatctgag agagacggtc agccagctgc
    18721 gtttgctctg aaaatttcag caaaagaatc agtgcccgca aggccgactc ataacggcct
    18781 acccgaacga tgccgtcata aggcagctcc agctgctgac agtaaccgca gcgacctccg
    18841 acaattccat acggactgac atcgtgtccg caccggcggc agtaatcccc tgacaccgct
    18901 tcggataagt cctgccaaca ggaccggcac agctgttcat cggcaggatg aatgctttgg
    18961 ccgcagacca aacatttatc cggccataat aaatgcaatc ccgcctgcca gacggctgct
    19021 gcactgttgc ccaaaacctt ccttgttccg atttccatgc tgattattat agaaaggatg
    19081 cagttttagg aaaagggctt atttcaagtc atacagggaa ttgtctggcg acaggaaacc
    19141 gattttaatc ttgttagagc agttgaaggc ttccagttga gatttataac gcgggtgtgt
    19201 gtccgtataa agctctcttt gagcggtaac atggccggcc gcccacccga tattggtttg
    19261 attcatatgt cggtagccct gcgtaccgcc agtccggaca tcccaatcgg tgtccccttc
    19321 ccaaacaaac ggcgaacgca tcagtttatt ggctccgccg gcataatgac cctctccaaa
    19381 tattacgcag gaggagggat ttctgacatg tgacgttctg gccggcatta cgatggactg
    19441 tccagaggcg tcttgagcgg atttgacgat tccggtaaag accgccggat tgacggtttc
    19501 gccctgtccg tgaccgatat agctggtatt gtagttataa ccggtatatg gatttccgct
    19561 ccagttatcg ctgcctttgt aggaaggaca ctgatgcact ttttccaacg tatcactctg
    19621 ccagagcaga ccagggcgaa tgagccggcg gccgtcagct gtaatgactg ccgtaaaatc
    19681 ccagcagaaa gaaaaggaaa ttgcttctga cagggcttga gaaccgcaat cgccgggcgg
    19741 ttcttgagga agcaatgccg aaccatcttg tccagccgaa aaacgctgat aataggcaat
    19801 cgggtagtaa tcatcgctat tcattgcata agccgcagca gccgcgcaca tttgcttgag
    19861 attgctgcgg cagtaaatcc cgcgggcctg ctcttttgcc agcagcagcg aaggagccgc
    19921 cacagccagc agaagtgctg taatgccaat aaccgtcagc agttcgatta atgtaaatcc
    19981 tttcctgtcg gtcattatct tactcgcatt tgtaggtaca ttccagccag ctagcggcca
    20041 cgatcgccaa atctgccata tccacccggc agtcccgtgt aatgtcgccg gaaggatacg
    20101 ggtgcgtggg atcaccgcag gccgctacat ccgccactgc atccacctga ccgcccaaag
    20161 caaacggacc ctgaccacct tccaaacgaa catactgaat ccagcgataa ccgctattag
    20221 gatccgggag caattggtca taatggggca gatcgttaag gtcaaaggac gtcccgcctg
    20281 ccgaaccggc atataaatca atcgcttcgg ccgctgtaag acctgaaaaa tcggccagcg
    20341 ttagattggg gtcaaccgga cgggtaaagt ccatttcaat atctgtccac cgggcatttg
    20401 cacggtccca ttgataggcc tgagtcggga ataaatcatc ggcccaggga ccattttcaa
    20461 atgtatgcca gatttgtccg tcttgactga cagaaacgat gatttgacca aatgcagtct
    20521 gtgcgggatt ggccaataga actgtattta gatttgtctg gtcggaaatt ttaacctgtt
    20581 gagaatactg aaaaaatgca ttcccaaata caatgaaatc ctgtccgaat ggattttgcg
    20641 gataatctac aaccggatgg tcaaacttaa cagttatcga ctcacccgga ttaattgtgg
    20701 tgataacacc ggcaccatcc agcgaaaggt tataggctgc ctctacaagc ttgacgcgaa
    20761 aggaaaccgg cagcgttgga tcccagagcc ggtcgtttag gcactgcacg gcgggtttgc
    20821 cgagaacagc gtttggattt cgataacggc tgccgttaaa gctgccttct gccgccgtta
    20881 cctcgacggc aaaatctgat gtcgccggaa cttggcagac agccattcct gcaatccagc
    20941 atatcaaaca taaatatctc attttcaccg gttttaaaaa tgcacaccag aggcgttggc
    21001 cctgctgtgc aaagatgtta gcgtttattt tctgttggat acaaaaagca aaccaatggc
    21061 caaaagtaca gccgccgtcg gttccggcac agccacagac gcactcggag cgccgccata
    21121 ccagccgttc tgggctgccc cccagctcca gccatcccag tcgccatcag ccaatactct
    21181 ggatgaggcc ccgatcatgg aggaaatcca tacacctgct tcattataaa aatagcccca
    21241 gaagccatcg gtttcccagc cgccgcacca gacatcaccc gactctgccg cagaccagcc
    21301 gtcaaaggca taaccggcca cagccatata gccgctgctg tcgaaattgc ttgcggatgc
    21361 tgttttgctg atgtcgaaga aaccatttcc atttttatca tatccaagtc cgcctattgc
    21421 aaagccgtaa ggccctgatc cgcccatcgc ataaaaatca gcatcagccg cacaaacggc
    21481 cagaaccaga tccgcaccag tagccgtacc atcccagcgg aatccccaga ccttaaccag
    21541 ctcgttaggt ccatcattcc aatcgataac aagggctgcc ctctgagtgc cctgaccgac
    21601 ccaaaaatcg atatcttcga atgtcatacc ggcaaatcct gcagcagcaa tagttaagcc
    21661 agccatataa acaacaaatc gcttcattga tgttctccaa agtttccata ttgctttttt
    21721 gaaaagtgcc gcagctgccg gcagattata agcagccgcg gcaagccctt cggcttattg
    21781 ttcaatcctc cgccggattg aacggtggaa ataacacaga ttttgagctt ttgggtctcc
    21841 tttctgaatc tgctctgggc agattcgaaa aaaatccata aaaaaagccg ctgtcggctt
    21901 attgctggaa cagcggctta tgagtatcga ttgcaaactc catgtcggaa aaggcaggtg
    21961 tccggacgct gcaaactggc cttgaccgac aggcgtgccg ctgttccata cgcgagaaca
    22021 aaccaacaca aaacaccttc aggcaggttt tctgacttgc atcctgcttg acaacccctt
    22081 cccatccgaa tagtggacag tgggacgatg ctgtcaagat ttgtgcgctc ggcacagcag
    22141 atacatacag cggcgcgacc gtcacggatt ttcaccgtgt tcccatttcg ttattcagcc
    22201 cggaaagtcc ggccgaacct gaaaatgctt ttccaataat ttcaaagaac tcacttaacc
    22261 atagcaagta cgggctatac ctgacaagta aaaatctggc tgattcttga caatagcaaa
    22321 ataccgtcaa tttatggtca tcgcaaaaat aagctaagtc tttttctgga aaagaagtta
    22381 acactaaata aacaggttaa aatacaacaa cagaatcagc cagatacttt tgaaaaacag
    22441 caatacatag aaacacttgg cgtatcatca actgtttttt cctcttaaat tgatcaaata
    22501 aatcaaacat ttttcacaaa aaccttatga ttataagacc tcttaaaata tatccaaccc
    22561 tgttatgttc cgatttgcac atcgcatttc acaatatttg cgctttttta tgttgaattt
    22621 tccactcaaa ttgaataaat aatgagaaac aaaatcaagc ccctttaatg tagaagcggc
    22681 atcaaggcga cttttttgtg aaaggagaag aggtcctatg acagaagcca aaaaaggtat
    22741 ggagtctctg ctgactgaac agcgaaagtt cccccctccc ccccacattc agtcaaaagc
    22801 ttggatttcc agtatggaag agtaccagaa aatgtgggag caatctattc ataatccgga
    22861 tgaattctgg ctggagcaag ccaaaatgct cacctggttc aaagagccaa ccaaagcgct
    22921 cgaatataca tggaattcca agaaccgcat catccggcat acctggtttg ccgacggcca
    22981 actgaatatt tcctacaact gcttagacag gcatttaacc acagcaaccg ccaataagat
    23041 cgctattctc tggcaaggcg aagaggacaa agccgtccgg aaattgacct atgccgaact
    23101 tcacaaagag gtatgcaaat tcgccaatgt attgaaatca aaaggcatcc agaaaggcga
    23161 ccgtgtagcc atttatatgc cgatggtacc cgaattggca attgccatgc tggcctgctg
    23221 ccgcatcggg gccattcatt ctgtaatttt cggcggattc agcgcagatg caatcaaaga
    23281 ccgtgttaat gattccgact gcaaaatgct gataacggcc aatgtatccc tgcgtgcagg
    23341 caagcatatc catttaaaaa atattgcgga tgaggcactg gccaagtgtc ctacaataca
    23401 aaactgcatt gttgttgaag tcaccaatga gccctatgaa atgaaacccg accgcgacca
    23461 ttggtatcat gacgaaatgg ccaaggtcag ttctaactgc ccagccgaaa acatggatgc
    23521 tgaagacccg ttgtttatcc tttacacctc cggctccaca ggcaaaccca aaggcgttgt
    23581 tcacactacc gccggatatc ttcttcacgc agcgatgtcc cataaataca tctttgatat
    23641 ccatgataat gatatttatt ggtgtactgc cgatatcggc tgggtcaccg gacacagtta
    23701 tatcgtatac ggacccttag ccaacggcgc taccagtctg atgttcgagg gagtgccgac
    23761 gtatccggat gccggacggt tctggcagat atgcgacaag ttcggggtta ccgttttcta
    23821 tactgcccct accgcgatac gtgctctgat gcgtctggga gatgagtggg tcaataaata
    23881 caagctggat accttgcgca ttctgggatc cgtcggtgag cccattaacc cggaggcgtg
    23941 gatctggtac tatgaaaaag tcggacacag ccgctgccca attgtagata cgtggtggca
    24001 gacagaaacc ggcggcatta tgatcacccc tctgccgggc gccatgaccc ttaaacccgg
    24061 cagcgctaca cgcccattct tcggagtgga tccggttgta ctgcgggatg acggaacacc
    24121 atgcaaccgc aatgaaggcg gcaaactgtg cattcgaaag ccctggccgg gaatgatgcg
    24181 gaccatgtgg ggcgcccatg accgatttat cgacacctat ttttctatgt ttgagaatgt
    24241 gtacttcacc ggcgacggct gccgcgtgga tgaagacggc gattactggc tgatgggccg
    24301 tatcgatgat gtcgtcaatg tctccggcca ccggatcgga acagccgagg tggaaagcgc
    24361 attggtcagc catcctaaag ttgcagaagc cgccgttacg cctatcccgc atgaaatcaa
    24421 agggcaaggg ctttatgcct atgtaacgct caaagagggg gtccaagaaa gcgaggacct
    24481 gaagaaagaa ctgattctgc atgtccgcaa ggaaatcggt gctatcgctg ctccggaagc
    24541 aatccagttt gctccggctc tccctaaaac ccgttcgggc aaaatcatgc gtcgcattct
    24601 gcgtaaaatt gcagaaaaag ataccagcaa tctgggcgat atcacaacgc tggccgatcc
    24661 gagcgttgta gaaaaactca ttgctggacg aggcaaatag acaactcctt cataatcttc
    24721 acggccggat gcgctgccag cagcgtgtcc ggcctttttc caaattcaaa acaggagatt
    24781 ggttatgaat gaacaaaatc cgcagtattc aataaaaaat ctggggtggc gcgttactct
    24841 tgcgggaatg ggcatcaatt tagcacttgg aattctttac acctggagcg tattcagcaa
    24901 ggccatccca gccgaatgga attggacgga aacccataaa tctttaccct atatgtttgc
    24961 ctgcctgatt ttttcgctgg taatggttcc tgccggacga atgcaggata aactcagccc
    25021 caagcttgct gcagcaatag gcggcatact ggtaggggtt ggttttattg tcgccagccg
    25081 caccacctct ccgctgggtt atctgatcgg ctttggtgta ttggccggcg ccggtatcgg
    25141 ctttggatac gcttccgcaa ctccgccggc ggtcaaatgg tttccaaagg ccaaaaccgg
    25201 tatgatagca gggattgtcg tttctggatt tggattggcg tctgtttata ctgcgcccct
    25261 gtcgaaatgg ctgatttccg aatataccat tcaaacagcg atgctgattc tcggcattgc
    25321 tttctttgta attgttgtcg tgctggccca gatgctgact cccccgccgg ccggttattc
    25381 agccgaaaaa aacagcagct ccagcggtgt atctgccgat cccaccaaaa aagagcattt
    25441 cactcccggt gagatgctgt caaccgcaca gttttatatg atgtggttta tgtacgcctg
    25501 cggggcaggt gccgggctga tggtgattgc caaattggca gctattgccg agattcaggc
    25561 gggtatcaaa cttggctttc tgctggtggc agcattggcg atcggcaacg gtgcggggcg
    25621 gattcttctg ggtatgctca gcgacaaaat cggacgcaaa gccactctgt ttctgtgctt
    25681 tgtcattcag gcagccgcta ttatgctgtt gtctgtgaca aagcagggaa cttttcttgc
    25741 atctgttccg attttaactg tcttatccgc acttatcgga gccaattacg gggctaattt
    25801 agcgctgttc ccttcgctga cgaaagatta ttacggactc aaaaatttcg ggataaacta
    25861 cgggctggta tttacagctt ggggcgtcgg tggctttatg ctctcgtatc tggctggcag
    25921 gatgtacgac atttacaaga catttgcaat cgcttattac ggggcttcta ttctgctgat
    25981 actggcggca gcatgcgttt tctttattaa gccgccgcac cggcaaatat ccaatcctca
    26041 ataagcagct ggattgttat tggcagtatc tgtacaaaaa gctaatcagt tagccagggg
    26101 atttcaatct attgactggc tgccggtctg cttgcggcac gatcgcacaa gattcggata
    26161 ttttcgagag cctcttcttc cggtaatatc tgtttgaaca aggccaatgc ttcttcccgg
    26221 cgtcccagaa gcgacagtgc cgcggccatg ttggcccgat agcgggtatt ggtgggcata
    26281 acagctgccg cttcggtgaa atagtccaag gccttggcat agtctttacg aatcagccag
    26341 cacatcccca tattgttcag caatacggga tcgcgcggac tgattttcag gcccgctgac
    26401 agcgtttcca tcgcttccgc aagccggcgc tggcgcatct gcattgctgc caagtcgtta
    26461 taagccggaa gaaaatcggg atattccctc tgaatccgct tgaacagctg ctcggcagaa
    26521 gcatcgcgtc cttggctgga cagaatatcg gccattagat acagtgtttt tgctgtcggc
    26581 ggacggtcgg gattaaatgg atcctccata accggtttgg cagttttccg attatattgc
    26641 ccgtgctgcc ccgcacagcc gcagataacc gcggcacaca aaaccactgc aatctctctt
    26701 ggtttgaaca ttacggtatc cttctgggtt attgcattct caattcggtc ataccggttg
    26761 aagctgtatc cttgaaagtt gttgtttgag cattttccag aatctcaatg accgctgttg
    26821 agggaattcc ttctccgccg gcaaaaccgc tgccaatctg gattttctca ttggggatgc
    26881 cggcctgcag cagtttttca taaacagtct gtgctcggct ctgatacagc agaggttcag
    26941 tttgcccctg aaccagtacg attttccccg gattctgccg gaaatgtgca gccagaaccg
    27001 ccaaatccct ttcacccagt gcattaagct gggcgctatt gttgacaaaa tgatagggga
    27061 aaagcgtatg ctgtgcaata atagcgttct gtattgccaa atctgaatag gacgctgtca
    27121 acttttgatt cactgtgttc tggtcgatcg gggcaggccc tataatcttt tcctcaccgc
    27181 agccgtttag aatcattccg cagacaacac caagcaataa cattgttttt ccgcatttca
    27241 ttttgaactc tcctgcttaa gaaggttttt aaaacgccgt ttttaacgcc agtattgccg
    27301 gccctaccat cacaattaaa accgccggaa aaataaacag aatcagaggg aaaagcattt
    27361 tgacactcat tttttcggct aattcctgag cagcaaaatt tctctcttca cgaatagact
    27421 gagcgaatac ctgaagggtt tcagcaatgc tggtgccaaa gcgatcggtc tggattaata
    27481 tggccgccaa tgattttaat tcgtccacac ccgttctctc cgccatatgc ttcattgctt
    27541 cgatgcggct gacacccaaa tgcatctcaa aattagccag agacatagcc ccggacagca
    27601 cactgctgac ctgacggatt tcatcggcaa caatgttcca agccatgtcc agccccagtc
    27661 cggaggacac gcagatttcc agcagatcta ccgcttccgg caggtggcgg tagatctcat
    27721 cgcggcggtg tttcatccgg tagagaatat acaaattcgg caggaaaaat gccgctgtcg
    27781 cacccacaaa aatcagggtc agacgagaac tgagcatcag ggtttcgctg tcaaaaggaa
    27841 gcagcagcag cgcagtaata aaaaaaccga ttacaaaaag aagaactttg atgccggtat
    27901 aaaccgtcgg cgcataagga ttcagataac cggcctgaat catctgttcc cacagcgatg
    27961 aactagcgcg tccatgggaa gcaatattgc ccaatttcac cagaaatgaa gacaaactcc
    28021 gcttgggggg tttttctgca aacggatcat cttcaaaaac tcccagcatg cggatttcca
    28081 ccgacttttt cttctggaga tgcgccatat agagcagaat agataatcca attgaaaaca
    28141 cggcgcagaa aatcagtatt ccaataataa cagcagattc ggaatgaaga aactccatca
    28201 tattgttcta tcgcatcctt tatcctaaaa atcgattttt gccattttgt tcatgaccca
    28261 cgagcccagc agcaaattga caatcatcag tatcagcaga acattgccgc cggccgttgt
    28321 ataaagcggc ttcatatatt cgggatcaac ggcattcagc agaaaaaaca tcagaatcgg
    28381 catggcaatc agaatcacct tgctgaactg cgtctgcgcg gtaagaaccc gaatccgctt
    28441 attaagccag atgcgagagc gaataaccga agcgagggta tccatcagat cagctagatt
    28501 accgccgctt tgcagctgaa caatgacgga tgtcgaaaac agtttgaatt ctgtatttcg
    28561 aacctcccgc gcagccttct taagcgaatc tttcaagtcg cttccaaatg cctgttcctg
    28621 ataaatctgc cggaaaaggc cgcccagcgg gtcgccgatt tcctcgccaa tcagctgaaa
    28681 agctcccacc aaaggatgcc ctgcgcgaag cgcccgcgcc gccacgccca gtgcatcaac
    28741 cagctgcctc tcaaacagat tcgcacgatg cataatccgt ttctgggcat actgcacaaa
    28801 taaataaagc gtaataaaca cgccgcagac agccagccaa ataccgccga atatattgta
    28861 aaccatcacg aagactaacg ctgccagacc gactacgccc agagctactg tacggaatgg
    28921 cactttccat cctgcatccc tcacccaata ctccagcttc tttccgaggc caatggagtc
    28981 tttttccaca gtttcctgcg gcatctgccg aatctgcaga tcgcgccata gacgaagtgt
    29041 ttgggaatcc tcctgaccgg ccttggaaat acccagacgc cgctgcaccg ctttgagctt
    29101 cagcaggtat cttcccagcc acagaagcac cccaatgcac cagaccgata aaaccaatcc
    29161 gaaaacggta acgctgatga ttaaattgat ggtctttgga tccatagtcg cctgttaatg
    29221 cacatccttt tttgcagagg ctgtttccaa tacgcggcgc tggaaaaggg tcggaggcag
    29281 ttcaatgccc tgagcaatca atttgtcgac cagacgagga cgaacgccgc agctttcaaa
    29341 gcgtccaaac gcattgccgt tctcatccac tccctgctgc tgaaagcgga aaatatcatt
    29401 cagacagacc gtttctccct ccatgccggt tatttctgca atattgataa tttttcttgc
    29461 accgccaacc aaccggccga tttgaatcag aatatccaga gcagagctga tctgctcacg
    29521 aatggctcga acagggaaat tcattccggc catactgacc atattttcga gccggcggag
    29581 agcatcacgc ggactgttgg catgtatggt agtcatcgac ccctcatggc cggtattcat
    29641 cgcctgaagc atatcaaggg cctcatcgcc gcgcacttcg ccgatgataa tgcggtctgg
    29701 acgcattcgc aaactattcc gcacaaggtc gcgctgcgtg atttgacctt tgccttcgat
    29761 gttgggcggg cgggtttcca gacgaaccac atgctcccgc tggagctgga gttccgccgc
    29821 gtcttcaata gttaccacac gctcgccagc aggaatccag cgggataatg cattcagcag
    29881 cgttgttttg ccggttccgg ttccgccgga aataaggatg tttattttgc actggacgca
    29941 ggactcaaga aaaaatgaca tttcctcagt caatgtgccc agttccagca gcgtatggat
    30001 atcgatggga ttggtgccaa accggcgaat gctgagggcc ggtccatcca gagccagcgg
    30061 cggaataata gcattaacgc gcgaaccgtc aatcaggcgg gcatccagca tcgggctgct
    30121 ttcgtcaatc cgccggccga cattggttcc gatacgctga atcacccgca tcagatgggc
    30181 atcatcctga aacgtaatat ccgttcgctc cagacgcccc cgccgctcta tatacacctg
    30241 ctgatacccg ttgaccaaaa tatcgctgat ggtaggatcg cgcagaagcg gctccaatgg
    30301 tcccagaccg aaaatctcat ccaatatttc atctaaaagc tgctgctttt ccggccctgt
    30361 aagagggtat ttttcctgca tcaggagagc atcaatcttg gcggtacact ccttctgcag
    30421 ctgctctatg gggaaacgct gtgcctcctt caggtctaac agtcccagca gccgctgatg
    30481 cacccgggac ttaatccgat gcaacgctat ttttttatca ctatcaaaac cgttcattcc
    30541 cgcatctgtg ccagtattca ttgtgccgcc tgttttttgg tgtcttaccg tttctcttcc
    30601 gatttgactt cctgaataac tgccgccagc tttcggaggt ctttgcgaat cgccgatcgc
    30661 ggagcacctt cagccagcgt ctggccttta ttgagacacc gcatagcatt gcgccaatcg
    30721 ctgcggacca gatacatcgt atcggttccc agcgcgcgtt tgccgtcatc aatccttagc
    30781 agcggccccc gtttggcaaa gcgatttgcc agaggaatga tcttttcatc gggcaatccc
    30841 agagtcctga acatggaaat aatcgaacgg gcaaacgcca gatccttaag cgtcaattga
    30901 aaaacaacaa tagtaaaatc gctctgctcg ctcaattccc gaaccgtctg ctcatccatt
    30961 cgaggcgcat caatcaccgt ataggcatac gcttcccgac aggcctgcag aaccattcgg
    31021 agactttcgg gtttgatatc aatgcccgtc ttgcgggatg cccctgaacc tttcaacagc
    31081 acgtgaaaat tttccttgta gcagcaggaa ctcgactgaa tcagatgttt gtcaattcgg
    31141 ccctcatagg tcagtacatc atcaatgccg tattccgatt tgacatccag ataagctgcg
    31201 gcagcgccgt agcagcgatc gaggtcaatc accaagaccg gtttggatgt ggagacatgc
    31261 atttccatag ccagattcag ggctatggtt gtcgcaccac agccgccgct ggccgaaaaa
    31321 actgtcataa tcgagccggt tttgcgtttg gcgccggtat gcgtcagcag ccgtgccata
    31381 actccggaca gttcgctgtg aatagagcct ttttggagaa aatgtctcgc tccggcctgc
    31441 atggccgaaa gaatcaactc ctgcgtaatc tgttcggaaa tgacaaccgt gcacatcttc
    31501 ggataatttc gaatcagctc ttccagctcg ctgagcgtct cctgcggggc cggatcaata
    31561 tcaacaattc ctgccgcagg ctcgccctcg tcaagatagg ccggaacatg cgagatttcc
    31621 ggaaacacct tgtcgatttc aatcggcgag ccgttgcggg caactgtttc aatatggctg
    31681 accgtcttgg ggtctttcgt gaccagaaat atttttgttt ttggagtcat gaatatcacc
    31741 tcatctatgg cattatcatc ttggtttctt gtgtcatccg catgggcgga ttcctatcca
    31801 ttactcccct gttcatccag ttattctgcc tgcagctgtg cccggctgag tggagccggc
    31861 gattgatacg aatcccaagc accggggccg ctgacttggt ccaaaccgag acgcttgagc
    31921 caatctgcat ccactccatc cagaccagcc ggctgacggg attctatacg gccttccaaa
    31981 tacaattccc aatcgctggg agctttatgg aggaatccgg gcagcggcga tgttttcgga
    32041 tcaatatcca gcggcgatac cagattagcc gtaaccagaa tcaccagctc ggtttcgccc
    32101 tgctggtagc gtgtcgaacg gaaaagaggg cctaaaacgg gcagattccc aagtcccgga
    32161 atagccgaat tggcggcact gatggtattt ttaagaaggc cggcgatagc aaaactctgt
    32221 ccgctcttga gttcaaccgt tgtttcagtc ttgcggctga gcactcccgg cacaaccgtc
    32281 ccattgatgg aggtgccgtt ggtataatcc agctcactga cttccggtgc ggtataaagc
    32341 cgaatggtat tgtcgcctag aacgacaggc ttgaaattca gccgaacacc gtattcctta
    32401 tattcaatgg tgatggttgc ggcggttccc gtccccgatt gcggaacagg aatggggtac
    32461 tccccgccgg ccagaaaact tgcctcctgc ccattgaggg caatcagcgt aggattggct
    32521 agaatccgca gatactgatt ttcctgcaaa gcatccagaa agaatacaag gtcagcactg
    32581 gtaatatgac cgaaggccgt tgctggcgtg ccgacttcgt aggcattaat ggcaccgggc
    32641 cagaacgaaa cgctttcggc aatcgggcct ccgggagcaa caccgctgga aaagtcgctt
    32701 cctgcctgag tccagtcaac accgagagac cgcaatcctg tcttgctgac ttcggccaca
    32761 cgcacctgca gctcaacctg ctgtacgcca accacttccg acatatcgat aaacgggatt
    32821 ttcatcttac tgagatattc ctgaagctgt tgggcatgaa gcgcattttt atgctggccg
    32881 cgaatcacca gattggaacc ggattgactc agcatcaggg atgatgtcgg aaacaattga
    32941 ccaatatcgg ctttcagcgt atccatatcc agcgttactt ggactttctt ctgcacaacc
    33001 tgagtttcat cctcactcca aagaagaata tcggttgttc caacagcaag gccttgaatc
    33061 agtacctggt tggcagtcag cacctgcaca tcggcaatag ccggatcggt aacggcaacc
    33121 cgggacacct tccaaggcgt ctgcataatt tgcgattgat gatacacaat agccacttcg
    33181 ctgccggcag catctgcggc tattgctgcc atccccgggc ttgccagtat agccagcacc
    33241 attgccgcta tccaactatt catctgtacg cctgattgac tcgccgttgt tctcattgct
    33301 taatcccctt gcctgaaact tcccttcatt atttacatgc tcaacgccat aatcctgccg
    33361 taaagcattc ttattgagac gattcctcgg gaacctgtac ttcactggtt ttatttcccc
    33421 ggataactgt aatggctgta ctccgctgag tggttttact gccttcttca ggattgctgc
    33481 cgactaccgg gataaccggt cgtccgggtc cgctgagctg gccgctgttg agcaccatcg
    33541 gctggggatc caccgcttcc atatccagag gattccgcag caccagtgaa atggaaccgt
    33601 tattgatagc cagctgcaga gcttcggcct gtttggaatc aagttcaagg gtcaccgtca
    33661 gtttttggga ggtgcgggct gaggcggctg tcttatcctc tttggatgca gcagacagaa
    33721 tagattcacc ggcgattgcc aatactttaa cccgttccag cagggttgtt gccacggctt
    33781 cgcctttgga ttcgctccgc attgaaccag acagcttgaa tgtcgccaaa acgtccacaa
    33841 aacattgcgg atacaaaagg ccgccggaga cctgatcgct ggatacggta acgctgaccg
    33901 cccgcatccg cggagccaga atagcggcaa tttcctgcgg gcctccctgt gtgagaattt
    33961 ttgatttcgt aatcgcctgt cggataacca catccgaagt cagtactttg cccacaacca
    34021 gagccgtatt gctgtaggca tctgccgggg cttctttgcg gggaacttta ataacttcaa
    34081 catcttcaac cgtcagcacc ttccaagccg acaggttgtc tttggcaaca accacttcaa
    34141 catcctcttt aattgtcgct gaaccctgca gagccgaaac aagcactacc gcacacacac
    34201 ccgctataat gcccaacacc accagcagca caattgtcca tttcataaaa cgctccttaa
    34261 atcctttgag ccgataaaat ccttatcatt ctgaaagctg gagacagcat tagggccctt
    34321 cttttgacat gaccacccgt gctgttaaag atgtcggagt cggcagacca attaggggac
    34381 ccttgagcct caaaatatcc acattcgcgg caggaacaga aatctcaagg gtcaactcat
    34441 tgcctacacc cggattccac ccattcggga cagagattga aacgccattg gcaggaatgc
    34501 cgcattcggc caaacgcgtg gaaatcagcg ctgcaacttc tccgcttcga ttgcccggaa
    34561 ggatggcgat acgggcagcg tgacgtgtaa tattggtaat ctgctgccat ttgaaaaaaa
    34621 gccagccata tttgagggca ccaaatgtca gaattattga cttttcataa attaaaggaa
    34681 cgagcctttg atgaccgata ataat
//
LOCUS       DAOTBO010000022        32844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1112_length_32844_cov_6.338650, whole genome shotgun sequence.
ACCESSION   DAOTBO010000022 DAOTBO010000000
VERSION     DAOTBO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 32844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32844
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1112_length_32844_cov_6.338650"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1288
                     /locus_tag="PLE88_04465"
     CDS             212..1288
                     /locus_tag="PLE88_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM75755.1"
                     /translation="MVQAVALMCVFGLGVCFSLLGSISVKLMPRVNIDESRFGTLISG
                     FMFACLVASLVVGIVTDAIGYKWIAILGFVLTAICIFILATGKNYMAVLLPCLLLGFG
                     AMALNTAGNVMAPQVLFGGKDPAAASNVANVFFGLGLFLTPLIVSFLFRQTSYEKAVS
                     VLGIIVILPAIVALMAANYPSSSAKLDIAAAVKLLGQPAVLAAAVVLFCYIALESSFC
                     NWLPSFGKEVVKKENPSIDTAEADVSGQRLLSYFAIAMMVGRLATGLLPGRLNFDLTA
                     NGGWVIAAAAVVAGVVIFLMMSCSKGTQAALLAVLAGLSFAPCFPTTVGVTFKIQTGC
                     LRKSVWYHICYRIGRRGYCSQSPR"
     gene            1425..2186
                     /locus_tag="PLE88_04470"
     CDS             1425..2186
                     /locus_tag="PLE88_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOM75756.1"
                     /translation="MKRILFLIVVTLVWAGCRKQAPAEAPQSPSQPQTSDVQQADPAQ
                     PAASDSAAPKSEEKMVPLPLELPKPMFVGTPENLAGIENLEPDTKGPRPVFYVPEGVK
                     NLALNKPVTSSEMEPITGELSMLVDGDKDAAEGSVIELGPFKQWVQVDLGQSYNLYAI
                     VFWHYHKTPRVYFDVVVQVSDDPEFAMGVTTLFNNDTDNSLGLGAGKDMHYIDKAEGK
                     LVDAKGTKARYVRLYSQANNQNDYSHYIEVEVYGK"
     gene            2190..4673
                     /locus_tag="PLE88_04475"
     CDS             2190..4673
                     /locus_tag="PLE88_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOM75757.1"
                     /translation="MKTAVIPILLVVLAGAAAFRLWRLADRPMHADEAVHAEKFGALL
                     EKGLYCYDPDEFHGPTLNYFTLLSAWLRGEHTYENISEVTLRLVPAVFGIALILTPLF
                     FVQGLGWRAAFFGCILLAFSPLFIYYSRYYIQEILLLFFSAVFLGAVWRYLHCQKLYW
                     VILAGVALGLMHTTKETFVFAAGAGACAVLYTIWTGTGRCKGWHLLAGFCTAAAISTM
                     FYSSFGSNWQGIGDSFAAYAGWFQRAGSSSVHSHPWYYYLDLLTWIEFLEPITWNEDG
                     IAALGLIGAAAVFFQNNSLHVQAARFVAIYTLFLTVVYCAIPYKTPWNMMGFVYGLAL
                     LAGFTADRLLDAVQGCWSKFVFWTLLGLYGLASPVVQSWFLNFRYASDPINPYVYAHT
                     SKDVFEMVRQVQTAADTQTPISVIAAGDDYWPLPWYLRSFSKVGYWNHPDPSVCDVPL
                     ILAQARHEQELLNLLYSVPPAGQRHLYLPLFDKACWLRPGVEWRGYIRKDQWDKMFSV
                     SPILSDTSEEPMLMQSRPDKTQIANLLKFSHQAMNTTFEIFIQHTDGTYAGRAARAAF
                     REIDRLEAQLSRFVENSDISRINRLQPGQTAAVDEDTLNCLAAAKQAWELTGGAFDVT
                     IGRMIEVWKKQDSQQAAELLRQRPSMAMLELHPQTCSVGLLGEGVYIDLGGIGKGYAV
                     DAAGRILKEWGISRSFIHAGTSSVLALDAPQGQSGWKVTLTNPADETIVARLAMANEA
                     LSCSGLLRGSHIINPFTGQPVADRRACWIRLGPNAALADALSTAAMILPIEKLKALYE
                     TVDGISIMAVLIEPNGRTELLQLGQWPKD"
     gene            complement(4670..5443)
                     /locus_tag="PLE88_04480"
     CDS             complement(4670..5443)
                     /locus_tag="PLE88_04480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM75758.1"
                     /translation="MVELSIIIPAYNESRKIAGDILAADQFCISSGIAGQIIIVDDGS
                     TDGTADAARTAAAQIRTPCLVEQLKSNRGKGCAVRTGMLKSQGAFAAFADSGCCVPFY
                     KILTALDLIRSGQCQIAHGSRKLSGVHIVRPQSAYRKICSGLFHWFLIHDIKRLANLT
                     DTQCGFKVYQGEAARRIYAQSRIDGFMFDVEIILLALAAGYTIREFPIDWAWDPDSRL
                     KPAREAIRVFCDLLTLKRRFADVLKDSDGRIARAHSPVG"
     gene            complement(5620..6981)
                     /locus_tag="PLE88_04485"
     CDS             complement(5620..6981)
                     /locus_tag="PLE88_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOM75759.1"
                     /translation="MSITVEEIFETVNMTLRQNFDIRTVTLGINLKDCMDRDLDHFCG
                     RIEQRIVAMGRKLNAAADSLEKKYGIPIINRRIAVSPVSLLAEPLPCTPATAVRIAKT
                     LDHCAAQADINFIGGFGGYVQKGLTASDENLMNALPQALSQTQRVCSFFNLGTTQTGL
                     NMTAVERFGHILKDLAFRSKTGIGCAKVAVFANVPGDNPFMAGAHHGIGEPDYALNIG
                     ISGPGVVKAVVEQNPDCDLTELSEVIKRTVFKITRAGELIGREAADSLGVEFGIVDIS
                     LAATPAAGDSVAEIIEAMGICRMGLPGTTAALALLIDAVKKGGAMASGRVGGLSGTFI
                     PVSEDAGMIRAVKQGALTLEKLEALTSVCSVGMDMFAIPGSTPADVISAIIADELAIG
                     IANNKTTSVRVIPVPGKRPGQTVHFGGLLGTAPIMPVPRKAAAAFIRRKGRMPAPIQS
                     LRN"
     gene            complement(7059..8876)
                     /gene="asnB"
                     /locus_tag="PLE88_04490"
     CDS             complement(7059..8876)
                     /gene="asnB"
                     /locus_tag="PLE88_04490"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOM75760.1"
                     /translation="MCGIAGFNWNDPHLIEQMTERLRHRGPDDKGYYCDPLVSLGHRR
                     LSIIDLSERGRQPMGYNGLQIIYNGEIYNYRQLRSELESAGYRFSSDTDTEVILQAYH
                     RWGTDCVHRFNGMWAFCIYDSSKKLLFLSRDRFGVKPLYYFFDGRHFVFASELKAIRL
                     STGNFRINPAAVNYFFYQKYIGDGLTIYDQCSKLRPSENLIFDLEKREIRNYFYFKLS
                     EQIEQCSNQSVKERLDKVESLIRDAVSKRLVADVPVGSFLSGGLDSSVISAFIARQRE
                     NFRTFSIGFQDESYDEIPFSRKAAEYIRTKHHIEYLNIDESLIMRVISHMDEPLGDAS
                     ILPTALLSQMTRRYVTVCLSGDGGDEVFAGYDSYLAYHIAKFIPPVFVQFSRPLTHLL
                     PPSDRKLNLTFKMQKFIEDYHPHPARRHLDWMATFTDRNRRMLLDEHYIKTEDFIGPL
                     GRSDLTGIQLNDFQRYLPEDILKKVDLASMMHSLEVRTPFLDYRLVPLVLSLPDAYKI
                     RFFRTKWLLKNIAAGLLPNEIIHRPKRGFTVPISSWVKKSSFIRQFIFEKEFYSHGLI
                     NKNYACKLLDEHLNRQRDNARKLWLIFVFNYWHHTAGRQ"
     gene            complement(8864..10036)
                     /locus_tag="PLE88_04495"
     CDS             complement(8864..10036)
                     /locus_tag="PLE88_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM75761.1"
                     /translation="MSSQRSMKILMLNYEFPPIGGGGGYAHQCLLKEYSRHPELQIDV
                     LTSSPAIGLTIETFSDNIRLYRVGIHKANLHFWKKSEVLEWLFKAERQLNKLLSSNNY
                     DLAHAFFGFPTGWLTCRRRKILPYLISLRGSDVPGANPRLTLEYKLLGPVFRKIWRNA
                     SLLAVNSKGLARRAKAFLPDLDYPVIPNGVDTERFRPASDKPALSPFKLITVGRLTEV
                     KRLDLALEAVCKALSAGLDVELTIVGDGNLTTRLKQLADALEISRRIHFTGWLDPDTL
                     PEIYPVHHCFLLTSLNEGMNNALLEALASGLAVITTPCEGTDELVQNNGIVVEHAGPQ
                     NLADAIIKIAQDSAAYSQMCKTSRTIAERFSWSSAAELYIRYYHQLAASGDKQCAE"
     gene            complement(9993..10718)
                     /locus_tag="PLE88_04500"
     CDS             complement(9993..10718)
                     /locus_tag="PLE88_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM75762.1"
                     /translation="MHAPDNIEQLNAAGWQWALSSGREQLGNLEANLRFLEKARIVKP
                     GLKIHEAGCGIGTIVDHLRSQGCQASGSDIAANAVAYGKNKYPGIDLAVHSAEELPYE
                     DESFDVVLSFDVMEHLFDVDKHLREIRRVLKKSGCYAFQTPNKYVHAVYDTLRLGNLS
                     WKTYHPSLQTPHKLKARLHRHGFSVQFYKMNPGTLFLAKKLGRFAFLARFISILPFES
                     LPLCLQTNLYVVAKKHENPDAQL"
     gene            complement(10718..12847)
                     /locus_tag="PLE88_04505"
     CDS             complement(10718..12847)
                     /locus_tag="PLE88_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOM75763.1"
                     /translation="MVRRKIKSPHPRQPMSQSALSPIISTGLLASILITVLLGGIPFA
                     LGRYIEFKSPDPFDSAAYVYSAKHLLDGARMGIDEIPTAHPVTLLINIFGVKFFGFSE
                     TGPKIIQTILQTAALVFMFYALWRIFGSAPAAVSTILAAVYLSAPLIAKFGNVKEQYM
                     IALAVCSACSFLLYEDRRKRFWLAACGFFAILPFYCKPTGAAIALAITSYLLISRCIS
                     RHWKNLLTEFLLFLGGILIGLVVPASLFIWQKNIHSFLRTFPVVAGQITLVFLAVGTV
                     IVYAVHYAHTLHLIAQLKQIRRSIWLAGIAALLIAVTVSVAGVSAANRYWTQSRSYPT
                     VSAARDVASYIFDIPIVNASIKIYHIATGSLERLIIASGVKGGYVGYSWKAITLSKLA
                     PQVFRYYKALCVPILLALASICTALLSLKSPLEKRHQSVWMLAIWWITDMGLIWVSPH
                     SYEQYYLPLCASGAMLGSFAVWRWQQKLTSAANKMPWLFIGAAGVILLAVLSIPIFIG
                     QRYSPDTGADYIKNYGSRRRGFAEALARIKADTPYPWQLAGDYIRTHSKEQDTIYVWG
                     WVPGIYVRAQRMAGLPKAYEGNMHVTPPAELAAEIEQMKNSFEQAPPHFIVDSRKRHF
                     PFDRPPLELWPMVPARLFGNEKPRLLSSSPQETAAFDSSYAAFLEKQHGPDEAKRYEA
                     MKPLREFVMHNYSFAGQFGDHMVFEKK"
     gene            complement(12857..13819)
                     /locus_tag="PLE88_04510"
     CDS             complement(12857..13819)
                     /locus_tag="PLE88_04510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOM75764.1"
                     /translation="MPETGHPALSIVIPVFNEEENLRPLYAEIAAALKDFAYEVLFVD
                     DGSTDNSFFVLTDIQKNTSNVRLIRFRRNFGQTAALSAGFKYARGSIIVPLDADGQND
                     PADIPRLVSKLQEGFDIVSGWRKDRKDNAVTRTLPSRAANWLIGRITGVRLHDYGCTL
                     KAYRAESLKSIRLYGEMHRFIPALARWGGENVTEMVVNHRPRTRGKTKYGLNRTFKVI
                     LDLITIKFLASFSTKPIYVFGGLGVLCFIGSFISGITVLAMKLLQNYSMNRNPLLIIS
                     LILMTTAIQFILMGLLAEILVRTYHESQDRPIYVIDKIIEPQMS"
     gene            14012..15157
                     /locus_tag="PLE88_04515"
     CDS             14012..15157
                     /locus_tag="PLE88_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOM75765.1"
                     /translation="MKYHNTTKLFCILCAVLAVGSLAGCQRSETKTAELKTEPAPKTT
                     KPAPQTQVGQTARPSVSPAIQTPNAPSIKVENPSVNFGQVGPNSVHKAVYAFSNSGNA
                     PLSVTNVQSTCGCSLPTLIKNDQRFAIPLSEPVLYEPGQSGKVEVTFTAPSTKGLTTK
                     HLYIISNDPVTPRAELLINAEVTVKIAVSPENADLRFDKPNAGMPSLIVKSLDEQSFS
                     IRSISIPNDVMKIDFDPGVKKKEFVLEPVVALDKLQQYTTGVIQITTDHPQSGTLVVH
                     YAAQPKYEVSRPRIILQNVEPQVPVIKDVIIRSNYGEKVEIASVQSRQGYMTIESQEP
                     DGNNIKLMVKITPPAQGAQRYITDEMILTLKNGEELSIRCSGWFRVN"
     gene            15506..16447
                     /locus_tag="PLE88_04520"
     CDS             15506..16447
                     /locus_tag="PLE88_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75766.1"
                     /translation="MSPLVAQAKSFALRINPPPPPEPVKPKIEPKPAPTPAQASTQPA
                     QPPVPKVQASVKFDLLATVRYETAPEKSLALVSTGSKLEWFRQGEKVGQHEIKEIRDG
                     SVIFTQAGGKPQEIFVPAKTEVKSLLKNEQKAVSAVRGPTGIQDTPAAGPNGMQDQPG
                     VADAEAVRAARTRETAVSTRSTVDARIQRVRSAPPVPTVEEQKASIESSILGIHEIMA
                     RQDDNLSEEERRQENQMWAELLQALNQEKENLEKAAQTKPSESVPDNLPGRSDSQETT
                     AAAAEKNQDAPAVPQPAASSSAQTPPAEGPAEPNQTG"
     gene            16524..20672
                     /locus_tag="PLE88_04525"
     CDS             16524..20672
                     /locus_tag="PLE88_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOM75767.1"
                     /translation="MQSYREAGIGQRKQWLAGLCILFWLSAVLMGQSGGVLRSRVYKL
                     KHITSQQAKELLVQLNIGKSYNTLTRDVLIVTSDVGTDLLKATEIVPFLDQEPPVQIR
                     VLMTAADSACLPTVEMLAARLKTITFGTMNEAPVRGAAKAAIVDTVNGKLLAIGTVDV
                     LNEIEAAAADLGQQSQTSASAQKAESAAAEKPLEPNLPATLMPLAETNLPAQEEPNAL
                     TPQTSAFSTEKPLEEAARQIFEGVPQLSDVNAPSDSAPAAAAEKSPEEDFLSEELMKA
                     LADAEETARAAQSAAQPVQETAPVKEAQTQVPEPNAAQAKPQEAKSEPTQPEQTAPAE
                     PIDPMKVLQALMAQAQQEEKALVEEQAQAEAAQQAAASSQDKEHLEKLQTELAQLRQK
                     LADLEALAGQAAPSGNAAEAKVQSAGSAPIPAEIAEKELETVIDLPQEVELEALVDLV
                     GKQLGLNYMYDPTILKGQKVQLKIHGGKIKVKDTYALLESVLRFKGFIMTRRGQLVTI
                     MRDSELAKAEPVLRAPDEPIQPGDIVVSSLFTLQYIDAATAQNMLKTMNLGTNFTPLE
                     TNSLIVTDYAYRMDRIRQVLTMVDVPAAAKEYRFRTLKYIKPSDIVGKLKDLAGQLQG
                     VTLQISAAAATPAAATQTRTVTTRNPQTGQMETRQVPIPTPATATATPAPQTSAAVTG
                     AAKDTVFIDTDDRTNRILIVGRADQIALIEELVDVLDVPQYDLKYVREYIIQNVEAIE
                     VVNVLNELGLANVAVATQAAGRTAARTAARTVSPQAQQVQEAAVAVQRTPTAGTAASG
                     DQPYVSIRPATNSLLVNGTAEQHRAIELVIAHVDVVQKDQRTIRQYEIQYVDTQTIID
                     ALTDLGIIAPSTSASTGAAARGTSARTSSLGRTTAVAQAVPTAAEQAAAPMVLPTAEG
                     GSEVELTADYPQITVLQTTNSLLVYATPRQHDAIALVIAHADRQLDVTTTPYVVYALE
                     NQDPVELAEVLTKLIQETVEERAKTSSPEAKIQTAPASGTAAGGGAPPTLEEQKIRII
                     PDPMSYSLIVYANKRNQQWIGELIRELDEYRPQVLLDCTLVEITKDESFKYDLDVISK
                     TYNELSLRSPSPISTISGNFSQQQYGEARSKSGEFTGFYNSDAIQALLTAVQTKSYGR
                     VMARPKVLVNDNQEGEIKTENKTSIAQQKSIVQPATGTSPSYTTTDVSFSEYSEGVIL
                     KIKPHISKGDMLRLEISLSRTDFTERQDVTVAGQTYPRPPDLLSTDITTVATVPDGTT
                     IILGGLEGVDQQKASTKVPIMGDLPIVGGLFRGVNDKGSQSKLYVFVKANILRPSDQI
                     EGLDDIRRVSSKNRQAFEEMEKKFQEHQDWPGIKPKPMEPVTVLEEDDFEQLRQELKE
                     LNER"
     gene            20713..22413
                     /gene="gspE"
                     /locus_tag="PLE88_04530"
     CDS             20713..22413
                     /gene="gspE"
                     /locus_tag="PLE88_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOM75768.1"
                     /translation="MKELLIQAAQRSGVVDAQLLRDFFDQHADDQRRIDQILLTAPTF
                     TEDMVLRLFAEALGIPFLDEINPSDVPREFIEQIPAPYVQHHYLIGIRPQGGNGLIRV
                     IAANPLNMTAVDNISKMLRCPVEMGLSTRAAITAAIDVAYEQKNTVIEEVAEELDAQN
                     LDQLADEVSSAEDLLDVVNRPPVIRLVNDILFRALQMRASDIHIHPFETKIVVRYRID
                     GILYDTLTLNRNVLPLVISRIKVMAGMDIAERRMPQDGRCSVRIGQREIDLRVSTVPT
                     SYGERAVLRLLDKSTGILTLEQLGLWQEDKEIFDKMINRTHGVIFVTGPTGSGKSTTL
                     YACLNRLDSTVKNIMTVEDPIEYQLEGISQMQVAPKKGMNFVNALRHILRQDPDVIMV
                     GEVRDQETAAMAIQSSLTGHLVFSTLHTNDAAGAISRLLDFGVEPYLVSSSLVCVLAQ
                     RLVRRICPDCKQAYDPPEHELRDLGIIQKDKTPFYMGAGCAKCFDTGYRGRTGIYELM
                     VINDEIRDMIYSRQTAGAIKRKALEYGMQTLRMDGARKVLAGTTTLAEVLRVTQSDNI
                     "
     gene            22438..23652
                     /locus_tag="PLE88_04535"
     CDS             22438..23652
                     /locus_tag="PLE88_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM75769.1"
                     /translation="MPRFEYIAINADKKTEKGTITAESALAARKHLRSRGLHPMDVTQ
                     VRVEAARRTFKDVFARGARREVASFTKELSTMLNAGIKLTDALSVLTLQVSHPHLRAA
                     VTEIRDRVVTGESFADALAEYEQFFDVIYVSMMRVGEATGTLGDSLATMAGFMDKRQR
                     LEAKMTTVMMYPAILLVFCVIVVLVITIAVIPKITEQLVKTGQQLPWITSVIMKLSAL
                     LTSWWVLVIMAGIAGLVLLYKRLMRTQRGAYLRDKLLLGLPVVGKLIRQEIVARFTAT
                     LATLLGSGLSMAESLRVVSQVTGNVIMMNAIKQARERILSGADISTPLRDSGVIDPAI
                     AHMVTVGEKSGELEQMLRLISENLEADSDLRIERFSRVIEPVIIIFMAGIIGIIAYAT
                     LLPLFKYSVTQF"
     gene            23708..24136
                     /gene="gspG"
                     /locus_tag="PLE88_04540"
     CDS             23708..24136
                     /gene="gspG"
                     /locus_tag="PLE88_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOM75770.1"
                     /translation="MRQESKGFTLVELMAVLLIIALLAGLAATNFMGQTDKAKVITTK
                     ATMKTLHNAVNMFKLDTGRYPSEDVGLIELIEQPTDVEGWNPQGYLESTTLPKDAWKN
                     DFVYMLNPESGKPFVIISYGADGKEGGEGYDADLYSTDTD"
     gene            24102..24698
                     /locus_tag="PLE88_04545"
     CDS             24102..24698
                     /locus_tag="PLE88_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75771.1"
                     /translation="MMRTCTARIRTRPLFCTDSPAGFVLVEMLLVVGLIALMTGVAVI
                     SFGAMWGNLRFKRQAEQLLTAFQMAQDAAAQSDRRYAVVLDFTEQAFILRQFKSLDLQ
                     GMDPQEAIIQTVHFDDSLTLDYVLYDDLEDTRQSENVTEARFLAGRSGWQYGGKVVLR
                     DAEGRPWTIVIHRFAKPPELFEGEVEIYLPQYPEQVPF"
     gene            24734..25654
                     /locus_tag="PLE88_04550"
     CDS             24734..25654
                     /locus_tag="PLE88_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75772.1"
                     /translation="MRKTSNITPSGFTIAEVAAALVLLSLILSSVLVLMNRYAAAVVD
                     LQLHQQAFELARANMERLLSEPKLSDISDYGTSDTNPELEWQTVVEPFYEPVTNQMWI
                     RAVCSAGYMDTKGQYQNVELEHWITNLTAEQVKQILAQQKAEDAYLELLQEGQLTAIQ
                     ETTRAYLEQMGLDVEAYEKLMAQQRRKKLEYLSRNGFEGYQNFLGQLEEEENAFLEKI
                     GMNFDGYNEFARTYVPRTEASESFSVDLPQEGSETMQDTAKDASDTARPAERSDPSRK
                     PASDGGFNWDNVPPELVPLIEQLLGIKKNG"
     gene            25651..26433
                     /locus_tag="PLE88_04555"
     CDS             25651..26433
                     /locus_tag="PLE88_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75773.1"
                     /translation="MKRSKQNAFTLVETLIVLMLAALIVTAVLGIYQQVRGAAVAILE
                     RMEKNRLQTEILQKIAEDIDRLAAPGFDAAIRFRSKLDNGFRSSQLVLENSYYGSTNQ
                     KQTYERIIWQTSYDPQGKTLILYRMHDGLNVEDKVLEKDAASSPSAQMFIPVAAGVTY
                     FDIQAQQGENILGVWLSDTLPKAVRIGISFAPLQQLADGSIGVPEENILYRTVAVDRT
                     RAIPYEFVRKKLELPMEQDPNELLNEADPNTVSGKQHSGVKK"
     gene            26463..27878
                     /locus_tag="PLE88_04560"
     CDS             26463..27878
                     /locus_tag="PLE88_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOM75774.1"
                     /translation="MTADRSSGFAFLVTLIVLVVLASLAAGLSVRLTMAKRRQQYMVD
                     YQRARYGADSAMKYILSVLPAKNFPLQNRKDKPDFSDLYWMDQQEYGRFIADWAADAT
                     DEQIQAVLKPGASLRPAEQSNPKDLIAQLLALFGAEEKLNETEQSNRIDANIPDSTIS
                     SAAMEGFEVVELDPNDVQVPGPYGPPWPYVIEPIELEMGPCRITIVIEDENAKMPLSW
                     LVTSSAQANKRAENALKTFCEWMAWDKQSLARLQNIIETAMKEVAKKKMFTLNPSPIL
                     LRPVSQTPQTPAATPQTQRATTRMTTSPAAAQAAAQPAAQTRPPSAHATDFAKLFHSS
                     LLDLEALAEPLPNTGQRQESPLKYLSLWGAQRVNINTAPRHVLEAAFTLAIDPLTASQ
                     LAQSVIVQRREKPFRTAAELRELGRLDSETMTVLQNYITTTSTFFQVRVISRSGNARA
                     AAVATVIKEGRQMERLAVMYE"
     gene            27911..29335
                     /locus_tag="PLE88_04565"
     CDS             27911..29335
                     /locus_tag="PLE88_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75775.1"
                     /translation="MDEKGSLGLYISRGKATAVWVSADTQPSVKLLFHVVPQPGESQS
                     IALQAARTAMQQAAAFGEVFAAIDCGCYTQYFLHSEFEDYAQVAGTIKYDAEEAAAAD
                     AANLAVAFDITGRDSFGSKVTVYAADRQYLTDILMDLQEGGLDPTYMEPDAVCLGRAL
                     CQLTPIDEQADALFVILSEPNCYLIRPKQDYAPTVRTFLAGQGQDITAVLAREVVLLA
                     AAEEAQNPLTTLVLIGRTDAIDKTRLHQRTGLEIKTETPEKSLGIADAERAGCYELLA
                     AYGAALAGQTRGHKADFRQDFMPYQGRRKMLVGSLRTISISLTVLLTAVALFFQLKAF
                     QMRRYVSRMSEKTLLEHKAVTYGKLPPRGMTPSSNLRREYTRAKEMEEGLGPGDEKSV
                     PAKLTFFFEAVSKTPPGVDINIQQITITERSMKVKGDTNNKAGTMALFNEINKHPRIS
                     LTSERTSSSPDGRDTFEITIEPKK"
     gene            29348..29851
                     /locus_tag="PLE88_04570"
     CDS             29348..29851
                     /locus_tag="PLE88_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75776.1"
                     /translation="MKQMLKNPAFYYILLPVLAGLWLLTAGFIFYPRSVAAWEDSRKE
                     YESSREWIEKLVILQPKRLAFQVDPSAKSEEFDFTRTIYEFAQVFSISPNNYTSNVRG
                     LVKRAGRPSRSAAITIKSIDIERLAQFLSAMLLRWPELNCEVLSLEKNKAGKNDWKVE
                     LTLTYYY"
     gene            29945..31114
                     /locus_tag="PLE88_04575"
     CDS             29945..31114
                     /locus_tag="PLE88_04575"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOM75777.1"
                     /translation="MSKKVAILGSTGSIGRNALRVLEALQPDYEVIGLSAHNNIRLLA
                     EQASKFNPRYIAITDNPGQLALKQIFSGFQGQILTGPESLVELASLDEADIVLCAVVG
                     AAGLPAALAAARLGKTLAIANKEPLVIAGELLTEAARQSGAVLLPIDSEHSAIFQAMQ
                     AGQPDEIRRIILTASGGPFRQTPLEQIKNATIQQALAHPTWSMGPKITIDSATMMNKA
                     LEVIEAVWLFGVPVEKIEVLVHPESVVHSMVEFIDGSIIAQLGTPDMKVPIQYALTWP
                     KRMRGIGDRLQLSSLGQLHFETPNLERFRALELGFEVARTAGSAPVVFNAANEAAVAA
                     FLDGRIHFGQIVELIEDCLLSHSVQRHLTLDELLSIDGWARRFVAEALVRQTAAG"
     gene            31138..>32844
                     /locus_tag="PLE88_04580"
     CDS             31138..>32844
                     /locus_tag="PLE88_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOM75778.1"
                     /translation="MTNKQTTSQVAEKGGKNTPQIIVFVLAAAAVVIWGINNPSVALR
                     ILAVMLGFGGIVMIHEFGHFIVAKLGGIKVEAFSIGFPPVVVGIRKLKKGWRIRLLPK
                     IGQPQHLEEGDNETEYQIGLIPVGGFVKMLGQSDSGAAEATDDPRSYANRPIWIRIAT
                     VAAGVVFNAIGAVIIFIILFMNGINLKPAMVGQVVPNSPAQEAGLRPGDEIIQVDGER
                     FVDFEAVLLAPVLSVPGEPVTFVVREADSGRERKVRIVAEKQAGDSSNLRKMGIAPLT
                     TLTVEPQIARSPELVDEIYKLTGLYPGDQVKAVNGQPVDHPWDFARKVSGIFDSTVRL
                     TIARRWQSADGSEMLVDVDFPMQVGPVVENFRDEFDLAHFSRLVPRLKIEAVMEPTHA
                     DTLIRWFQTAILRRPLEPAAWDLLKAGDILLKVADVEYPNYKQLRELTTAYEGKPLPI
                     TVLRSGQQGDTLPIEIVVTPKARIGSKRIMIGFAAGLDLDNPVVGQVLSRTDQAEDLP
                     AVPAGAVITAINGRAVENFYQIAAMLQDNAGRSVSIDYQYNGQFGRTNLSVDAYEPVH
                     AQA"
BASE COUNT         8228 a         7919 c         8882 g         7815 t
ORIGIN      
        1 attggcgccg gtgctgagca tgctgccgtg ggtgggaact gacggacaga cttttctggc
       61 caaggtgcat caatggtgtg cattggtttt ttcacttgca gccatcacac aattgtatat
      121 gctgattcga atggaagtcc ttcgggatat gaaggcagaa tcacaatcgc tgtaaaataa
      181 tgcaggatgt cgattggaaa ggagtgtgag tatggtacag gcagtcgctt taatgtgcgt
      241 gttcggcttg ggtgtatgtt tttcactgct gggttccatt agtgtgaagc tgatgccccg
      301 tgtcaatatc gatgaaagcc ggtttgggac actgatttct gggtttatgt ttgcctgtct
      361 ggtcgcctcg ctggttgtcg ggattgtgac cgacgcgatc ggctacaagt ggattgcaat
      421 tctcgggttt gtcctgacgg caatctgcat tttcattttg gcgacaggca aaaattacat
      481 ggctgtactg ctgccctgtc tgctgctggg cttcggagcg atggccctga atacggccgg
      541 caatgtaatg gccccgcagg tgcttttcgg cggcaaagat ccggcggcag ccagcaatgt
      601 ggccaatgtg tttttcggat tgggcctgtt tctgacgccc ctaattgtca gttttctgtt
      661 tcggcaaacc agttatgaga aagcagtctc tgtgctgggc attattgtca ttctgccggc
      721 cattgtggct ctgatggcag ccaactatcc ttccagcagc gcaaaactgg atatcgcggc
      781 agccgtcaag ctgctgggtc agccggcagt actggcggca gctgttgttc tattttgcta
      841 tattgcactg gaatcatcgt tctgcaactg gcttccctca ttcggaaaag aggtggtcaa
      901 aaaggaaaat ccctcgatcg ataccgctga agcagatgta tcggggcagc gcttgctgtc
      961 ctattttgcc attgcgatga tggtcggacg actggcaacc ggacttctgc cgggccggct
     1021 caattttgac ctgacagcca atggcggctg ggttattgca gcggcggctg tggttgccgg
     1081 cgtggtcatt ttcctgatga tgagctgttc caagggtaca caggcggccc tgctggcagt
     1141 cttggcgggg ctgtcgtttg caccttgttt tccgacgacg gttggagtaa cgttcaaaat
     1201 tcaaaccgga tgtttacgga agtctgtttg gtatcatatt tgctatcgga ttggccggcg
     1261 gggttattgt tcccaaagcc ctcggtaatg ctgccaaagg tgcatccatt caaaaaagcc
     1321 tcaaactgct gctgccggcc tgcgggattt tgattgtttt agcaatcatt ttgggtaaac
     1381 tcaaaggaat tgcaggttaa tattgtttta ttgtggagtt gtcgatgaaa cgcatactgt
     1441 ttttgattgt cgtaacactg gtttgggctg gctgcaggaa gcaggcccct gctgaggcgc
     1501 cgcagtctcc ttcacagccg cagacttcgg atgttcagca agcggatccg gcccagccag
     1561 ctgcctccga tagtgctgct ccaaagtccg aagaaaaaat ggttccgctg ccgctggagc
     1621 tgcccaagcc gatgtttgta ggcacgccgg agaatttggc cgggatcgaa aatctggaac
     1681 cggataccaa aggtccgcgg cccgtttttt atgttcccga aggagtcaag aatctggcac
     1741 tgaacaaacc ggtaacaagc tcagaaatgg aacccataac aggtgagctg tcgatgctgg
     1801 tggacggcga taaggatgct gccgaaggca gtgtgattga acttgggccg tttaagcagt
     1861 gggtacaggt tgatttaggc cagtcttaca atctttatgc gattgtcttt tggcattacc
     1921 acaagacccc gcgggtgtat tttgatgtcg tggtgcaggt ctctgatgat ccggagtttg
     1981 cgatgggtgt aacaaccctg ttcaataacg acacggataa ttcgctcgga ttgggcgccg
     2041 gaaaggatat gcactacatt gataaagccg aaggcaaatt ggtcgatgcc aaaggcacca
     2101 aagcccggta tgtccgtcta tacagtcagg ccaacaatca gaacgactac agccactaca
     2161 tcgaggtaga agtatacggc aagtagtcca tgaaaacggc ggtcatcccg attctgctgg
     2221 tggtgctggc tggggcagcg gcttttcgcc tgtggcggct ggctgaccgg ccaatgcatg
     2281 cggatgaggc ggttcatgct gaaaaattcg gggcccttct cgagaagggc ctctattgtt
     2341 atgatcccga tgaatttcac ggaccaaccc tgaattattt tacgcttctc agcgcttggc
     2401 tgcgggggga gcatacgtat gaaaatatca gcgaagttac actgcggctg gtacctgctg
     2461 tttttggtat tgcgctgatt ctgacgccgt tgttttttgt ccaaggcctc ggatggcggg
     2521 cagctttctt cggctgtatc ttgctggcgt tttctccttt gttcatttat tacagccgct
     2581 actatattca ggaaattctg ctgctgtttt ttagtgccgt tttcctcggt gctgtctggc
     2641 gttatctgca ttgccaaaaa ctttactggg taattctggc cggtgttgct ttgggactaa
     2701 tgcatacaac caaagaaaca tttgtctttg cagcgggcgc aggtgcttgt gctgtgctgt
     2761 ataccatctg gacaggtacc ggccggtgca aaggatggca tctgctggcc ggattctgca
     2821 cagcggctgc aatatcgacg atgttttatt cctcattcgg cagcaactgg caggggattg
     2881 gcgattcttt tgctgcctat gctggctggt tccagcgtgc cggcagcagc agtgttcaca
     2941 gccatccgtg gtattattat ttggatctgc tgacgtggat tgaatttctt gagccgatca
     3001 catggaatga agacggcatt gcagcactgg ggcttatagg agccgctgct gtattttttc
     3061 aaaataacag cctgcatgtt caagcagctc gatttgtggc gatttataca ctgtttctaa
     3121 cggtggtgta ttgtgctatt ccctataaaa cgccgtggaa tatgatgggt tttgtttatg
     3181 gactggcgct gctggccggt tttactgccg atcggctgct cgatgcggtt cagggatgct
     3241 ggtcgaagtt tgtgttctgg acactgctgg gactttacgg gctggccagt ccggttgtac
     3301 agagctggtt tcttaatttt cggtatgcgt ctgatccgat taatccttat gtgtatgccc
     3361 acaccagcaa ggatgttttt gaaatggttc gacaggtcca aacagctgcc gacacccaaa
     3421 caccgatttc tgttatagcc gccggtgatg attattggcc gctgccgtgg tatctgcgga
     3481 gcttttccaa agtagggtac tggaatcatc ctgatccttc tgtttgtgat gtgccgctta
     3541 tccttgctca agcccggcac gagcaggaac tgctgaatct gctgtactca gttccgccgg
     3601 ccggtcagcg ccatttgtat ctgcccttgt ttgataaagc ctgctggctt cgtccgggtg
     3661 ttgaatggcg gggctatatt cgtaaagacc agtgggataa aatgttttct gtttcgccga
     3721 tcctttccga tacatcagag gagccaatgc tgatgcaaag ccgacctgat aagacccaga
     3781 ttgccaatct tctcaaattc agccatcagg caatgaatac gacgtttgag atttttatcc
     3841 agcatacgga cggtacatat gccggccgtg cggcccgggc agcatttaga gaaatcgatc
     3901 gtctggaagc ccagttgagc cggtttgtgg aaaacagcga catcagccgg attaaccggc
     3961 tccagccggg acaaacagcg gcggtagatg aggatacatt gaactgtctg gcagccgcca
     4021 aacaagcgtg ggaattgaca ggaggggcat ttgatgtcac gatcggcagg atgattgagg
     4081 tctggaaaaa gcaagacagt cagcaggcag ccgaactgct gagacagcgg ccctcaatgg
     4141 cgatgctgga gctgcatccg cagacgtgca gcgtcggcct gctgggcgag ggagtctata
     4201 tcgatttagg cggcatcgga aaaggttatg ctgtagatgc ggccggccgc attttaaagg
     4261 aatggggcat cagccgttca ttcatccatg ccggaaccag ttccgtgctg gctctggatg
     4321 ccccgcaggg acaaagcggc tggaaggtta cgctgaccaa tccggccgat gaaacaattg
     4381 tcgcccgtct ggcaatggcc aatgaagcgc tgagctgttc ggggcttttg cggggcagcc
     4441 atattatcaa cccctttacc ggccagcctg ttgcagatcg gcgggcctgc tggattcgat
     4501 tgggtccgaa tgccgccttg gccgatgcgc tttcaacggc tgctatgatt ttgcctattg
     4561 agaaactaaa agccctttat gaaacagtag acggaatttc cataatggct gttctcattg
     4621 agccgaatgg ccggacggag cttctgcaat tgggccaatg gccgaaagat tagccaaccg
     4681 ggctgtgcgc acgggcaata cggccgtcgc tgtctttgag cacgtcggca aaacggcgtt
     4741 tcagcgtcag aaggtcgcag aaaacccgaa tcgcctcacg ggccggttta aggcggctgt
     4801 caggatccca cgcccagtca atcggaaatt cacgaatcgt gtagccggca gccaaagcca
     4861 gcagaatgat ttctacatcg aacataaagc cgtcaatgcg gctttgggca taaatccggc
     4921 gggcggcctc gccctgatat accttaaagc cgcactgggt atcggtcaga ttggcaagac
     4981 gcttgatgtc gtgaatcaaa aaccagtgaa acagtccaga gcagattttg cggtaggccg
     5041 attggggacg cacaatatgc acgccggata attttcgaga gccgtgggca atctggcatt
     5101 gcccggaacg gatcagatcc agcgcggtta agattttgta aaaaggcacg cagcagccgc
     5161 tgtcggcaaa ggcggcaaat gcgccttgac ttttgagcat tccggttcgt acggcacaac
     5221 ccttgccccg attgcttttg agctgttcaa ccagacatgg cgtgcgaatc tgtgctgcgg
     5281 ctgttcgggc agcatcggca gtgccgtctg tgctgccgtc gtcaacaatg ataatttggc
     5341 cggcgatacc ggatgaaatg cagaactggt cagccgccag aatatcgccg gcgatttttc
     5401 ggctttcatt ataggctgga atgataatag aaagttcaac catattacag tatgcctgtt
     5461 tttatgctgc tttgaatact gtaggccata ttcgctttta aatcaatcct gttctgctaa
     5521 tttatatacc cctcgatgct gatttattta tacctgggtt accttcaatt cacgatggcc
     5581 ggtatctgcg ggttcttgag cggctgactc cgcctgcgtt tagttccgaa gagattgaat
     5641 aggagctggc atacggcctt tgcggcggat gaaggccgca gcggccttgc ggggaacagg
     5701 cataatcgga gctgttccca aaagtccgcc gaaatgaaca gtctgaccgg ggcgtttgcc
     5761 gggcacagga ataacgcgga cgctggtggt cttgttattg gcaataccga tagccagctc
     5821 gtcggcaata atggcgctga taacatcagc cggagttgag cccgggatgg cgaacatatc
     5881 catcccgacc gaacaaacgc tggtcagtgc ctccagtttt tccagcgtca aggcgccctg
     5941 tttgacggca cgaatcatac cggcatcctc gctgacgggg ataaatgttc ccgaaagtcc
     6001 ccccacacgg ccggaagcca ttgcaccgcc tttcttgact gcgtcaatca gcagtgccaa
     6061 ggccgccgtt gtaccgggca gacccatccg gcaaatgccc attgcttcaa taatttcggc
     6121 caccgaatcg ccggcagccg gggtggcggc taaggagata tccacaattc cgaattctac
     6181 acccagactg tctgctgctt cgcggccaat cagctcgccg gcacgggtta tcttaaaaac
     6241 ggtgcgcttg atgacttcag aaagttcagt taaatcgcag tcaggattct gctcaacaac
     6301 ggctttgaca acgcccggcc ctgagatacc gatattgagg gcataatcgg gttcgccgat
     6361 gccgtggtga gcaccggcca taaagggatt gtcgccggga acgttggcaa agaccgctac
     6421 cttagcacag ccgatgccgg ttttgcttcg gaaagccaag tcttttagga tatgtccaaa
     6481 ccgctcgaca gcagtcatat taagccctgt ctgtgtggtg cccaagttga aaaacgagca
     6541 gacgcgctgg gtttgagaca aggcctgcgg cagagcgttc atcagatttt catcggaagc
     6601 ggtcaggccc ttctggacat agcccccaaa gccgccaata aagttgatgt cggcctgagc
     6661 cgcgcagtgg tccagcgttt tggcaatgcg cacagcggtt gccggcgtgc agggcaaggg
     6721 ctcggccagc agagaaacag gactgacagc aatgcggcgg ttgattatgg ggatgccgta
     6781 ttttttttcg aggctgtcag cggcggcgtt gagttttctg cccatggcaa cgatgcgctg
     6841 ctcgatgcgg ccgcagaagt ggtccagatc ccggtccata cagtctttga gattgatgcc
     6901 taaggtgaca gtgcggatat caaaattctg ccgcagcgtc atattgacag tttcaaaaat
     6961 ttcttcaacg gtaatactca taatttctcc tgctgattca ttttcagttc tgggctattg
     7021 tagccgccga aggctgcttt tgcaaaagca gcttgctgtt attggcggcc ggctgtatga
     7081 tgccaataat taaagacaaa aatcagccat aacttgcggg cattgtctct ctgtctgttc
     7141 aggtgctcat ccagcagttt gcaggcataa tttttattga ttaaaccatg gctgtaaaac
     7201 tctttctcaa agataaattg acgaataaaa gatgatttct tcacccaact cgagatagga
     7261 acagtaaacc cccgttttgg gcggtggatg atttcattcg gcagcaggcc agccgcgata
     7321 tttttcagca gccatttggt ccgaaagaat cgtatcttgt aagcgtccgg cagggataag
     7381 accagcggca caagccgata atcgagaaag ggcgtgcgga cttccaatga atgcatcata
     7441 gaagccaaat ccactttttt taagatatct tccggcagat aacgctgaaa atcattcagc
     7501 tgaatgccgg ttaaatcact ccgcccaaga ggaccgatga agtcctcggt tttgatataa
     7561 tgttcatcca gcagcatccg gcgattgcgg tcggtgaagg ttgccatcca gtccagatgg
     7621 cggcgggcag gatgggggtg ataatcctca ataaattttt gcatcttgaa agtcagatta
     7681 agtttcctgt cggaaggcgg cagcaaatgt gtcagcggac ggctgaactg tacgaataca
     7741 gggggaataa actttgcaat atgataagcc agataagaat catatccggc aaaaacttca
     7801 tctccgccgt ctccagaaag acagacagtt acataacggc gcgtcatttg cgacagcagt
     7861 gctgtcggca gaatggatgc gtctcccagc ggctcatcca tatggctgat gacgcgcata
     7921 atcagtgatt catcgatatt tagatattcg atatgatgct ttgtgcggat gtattcggcc
     7981 gcttttctgg aaaaagggat ctcatcgtag gattcatctt gaaagccgat ggaaaacgtt
     8041 cggaaatttt ccctctgacg ggcaataaaa gccgatatga cggatgaatc cagtcctccg
     8101 gacagaaaac tgccgaccgg tacatctgcg accagccgtt tggagacagc gtcgcgaata
     8161 agactttcca ctttgtccaa ccgctctttc acgctttgat tggagcactg ttctatctgt
     8221 tcggaaagtt tgaaataaaa gtaattgcga atttctcttt tttctaaatc aaaaatcaga
     8281 ttttcagagg gcctcaattt gctgcattgg tcgtaaatcg ttaagccgtc accgatgtat
     8341 ttctggtaga aaaagtaatt cacagcagcc ggattgattc ggaaattgcc tgtactcagc
     8401 cggatggctt tcagttcgga agcgaaaacg aagtgccggc cgtcaaaaaa ataatacagc
     8461 ggcttcacac cgaaacgatc gcgtgaaaga aaaagcaatt ttttggaaga atcataaata
     8521 cagaatgccc acatcccatt gaagcgatga acgcagtcgg ttccccatcg gtgataggcc
     8581 tgcaggatga cttcagtatc tgtatcggaa gagaaacggt atcctgccga ctccaattct
     8641 gaccgcaatt ggcgataatt gtatatttca ccattgtaaa tgatctgaag accgttatag
     8701 cccatcggct ggcggccccg ttctgacagg tcaattatag acagccggcg gtggccgaga
     8761 ctgaccagcg gatcgcagta atagcccttg tcatcgggac cgcgatgacg cagccgctct
     8821 gtcatctgtt caatcagatg ggggtcattc cagttgaatc cggctattcc gcacattgtt
     8881 tgtcgcccga agcagcaagt tggtgataat atcgtatata cagttcggct gctgaagacc
     8941 acgagaaccg ttcggctatt gtacgcgaag ttttgcacat ttggctataa gcggccgaat
     9001 cctgtgcgat tttgataatg gcatctgcta agttctgcgg accagcatgt tccactacaa
     9061 ttccattatt ttgcaccagt tcatcagtgc cttcacaggg tgttgtgatg acggctaaac
     9121 cgctggccag cgcttccagc agagcattgt tcattccttc attcagactg gtcagcagaa
     9181 agcagtgatg aaccggatag atttccggca aagtatcagg atccagccat ccggtaaaat
     9241 gaatcctgcg gctgatctca agagcatcag ccagctgttt aagacgggtt gtcagattgc
     9301 cgtcgccgac gatggttaat tcgacatcta aaccggccga aagtgccttg cagacagctt
     9361 ccagtgccaa gtccagacgc ttgacctctg ttaagcggcc tacggtaatc agcttaaacg
     9421 gggacagagc cggtttgtca gaagccggcc taaaacgctc ggtatctact ccattgggga
     9481 taactgggta atccagatcc ggaagaaagg ctttggcacg ccgagccaaa cctttgctgt
     9541 tgactgccaa aagcgaagcg tttctccaga ttttacggaa gacaggcccc agtaatttgt
     9601 attccagcgt cagacgggga ttggccccgg gtacatccga tccccgcagc gagataagat
     9661 agggcagtat cttgcggcgg cggcaagtca gccagccggt cggaaagccg aaaaaagcat
     9721 gagccaagtc ataattattg ctggataaca gcttgtttaa ttgccgttct gctttgaaca
     9781 gccattcaag aacttcgctt tttttccaaa agtgcagatt ggccttgtgg atgccgactc
     9841 tgtataagcg aatattatct gaaaatgttt ctatggtaag gccgattgcc ggagaactgg
     9901 taagaacatc gatctgcagc tccggatggc ggctgtattc tttcagcagg cattgatggg
     9961 catatcctcc cccgccgccg ataggcggaa attcataatt gagcatcagg attttcatgc
    10021 ttctttgcga cgacataaag gtttgtttgc agacacagcg gcaaagactc aaaaggaaga
    10081 atagaaataa agcgtgccaa aaaagcaaac cgccctaatt tttttgctag aaacaaagtt
    10141 cccggattca ttttataaaa ctgaacagaa aacccgtgcc tgtgcagccg tgctttcaac
    10201 ttgtgcggag tctgaagaga cggatggtac gttttccagc ttagattccc aagacgcagg
    10261 gtatcataaa cggcatgcac atacttgttg ggtgtttgaa aggcatagca gccgcttttt
    10321 ttgagaaccc gacggatttc acgaaggtgc ttatccacat cgaaaagatg ctccattaca
    10381 tcaaaactta aaaccacatc aaaggattca tcctcatacg gcagttcctc cgcagaatgc
    10441 accgccaaat caatcccggg gtatttattt tttccgtaag caaccgcatt ggcggcgatg
    10501 tcgcttccgc tggcctgaca cccctgcgac cggagatgat caacgattgt gccgattccg
    10561 cagccggctt catggatttt cagtcccggc ttaacaatac gggctttttc aagaaaccgc
    10621 aaattggcct ctagatttcc gagctgttcc ctgccgctgg acaaggccca ttgccagccg
    10681 gcggcattca attgttcaat attatccggt gcgtgcatta ttttttttca aacaccatat
    10741 gatctccaaa ttgtccggcg aagctgtaat tgtgcatcac aaattcccgc agtggtttca
    10801 ttgcctcata gcgtttggct tcgtcgggcc catgctgttt ttcaagaaaa gcggcatacg
    10861 aggagtcaaa agcagccgtc tcctgagggc tgctgctgag cagtcggggt ttttcgttgc
    10921 cgaacagtct agccggcacc atcggccata gctccagcgg cggtcgatca aacgggaaat
    10981 gccgtttgcg gctgtccacg ataaaatgcg gcggtgcctg ctcgaagctg tttttcatct
    11041 gctctatttc ggctgccagc tccgccggcg gtgtgacatg catattgcct tcgtaggcct
    11101 tcggcagccc agccatacgc tgggcccgca cataaatgcc gggtacccag ccccaaacat
    11161 aaattgtatc ctgctctttg ctgtgcgtgc ggatatagtc gccggcaagc tgccaaggat
    11221 agggcgtatc ggccttgatg cgggccagcg cttcggcaaa accgcggcgg cgggaaccgt
    11281 aatttttgat gtagtcagca ccggtatcag ggctatagcg ctggccgata aagatgggaa
    11341 tagacagaac cgccaaaaga atcaccccgg cagcgccgat aaacagccac ggcattttat
    11401 tggcggccga agtcagtttc tgctgccacc tccagacagc aaaactgccc agcattgccc
    11461 cagatgcaca cagcggcaga taatactgct catacgaatg aggactgacc caaatcagcc
    11521 ccatatcagt gatccaccaa atcgccagca tccagacaga ttgatgccgt ttttcaagcg
    11581 gtgatttgag agacaacaac gccgtacata tagaagctag tgccagcaga atcggaacac
    11641 acaaggcctt gtagtagcga aaaacctgcg gagccagttt gctcagagta atggctttcc
    11701 agctgtatcc tacatagccg cctttaactc ctgaggcaat aatcagacgc tctagactgc
    11761 cggtcgcgat gtggtaaatt ttgatagaag catttactat cggaatgtca aatatatacg
    11821 aagcaacatc gcgggcggca gaaaccgtag gataactgcg gctttgcgtc caataccggt
    11881 tggcggcaga tactcccgcc actgaaacag tcactgcaat cagaagcgcc gcaatgccgg
    11941 ccagccagat gctccggcga atttgtttaa gctgggcaat caagtgcagt gtgtgggcat
    12001 agtgaacggc ataaacgatt acagtaccga cagccagaaa taccagtgtt atttgaccag
    12061 caacaacagg aaatgtgcgt aaaaaagaat ggatgttctt ttgccaaata aacagagatg
    12121 ccggaacaac aagaccgatg aggataccgc ccagaaacag aagaaattct gtcagcaggt
    12181 ttttccaatg acggctaatg cagcggctta tcagcagata ggatgtgatg gccaaagcaa
    12241 tcgcagcacc ggtcggcttg cagtaaaacg gcagtatggc aaaaaatccg catgccgcaa
    12301 gccaaaaccg tttacggcgg tcttcataca gcaaaaagct gcatgcagaa cagacagcca
    12361 gtgcaatcat atactgttct ttgacgttgc cgaacttggc aatcagcggc gcagacagat
    12421 agactgccgc aaggatagtg ctcacagccg ccggtgcaga accaaaaatt ctccagaggg
    12481 cataaaacat aaacaccaat gcggcggttt gcaaaatggt ctgtatgatt ttcggtccgg
    12541 tttcgctgaa gccgaaaaat tttacgccga agatgttaat cagcagggtc actggatgag
    12601 ccgtcgggat ttcatcgatc cccattcggg caccgtccag aaggtgtttt gccgaataca
    12661 cataagcagc gctgtcaaac ggatccggcg atttgaattc gatatagcgg ccaagcgcaa
    12721 agggaatgcc ccccagcaaa acggttatca ggatcgacgc cagcaggccg gttgagataa
    12781 taggggacaa agcggactgg gacatcggtt ggcggggatg agggcttttt attttgcggc
    12841 ggaccatagg caatcgtcag gacatctggg gttcaataat tttatcaata acataaatag
    12901 gccgatcctg cgactcatga taggtccgca cgaggatttc agccagcagg cccatcagaa
    12961 taaattgaat agctgtcgtc atcaaaatca gcgagataat cagcagcgga ttgcggttca
    13021 ttgaataatt ctgcagcagc ttcatcgcca atacggttat tcccgaaata aaggaaccga
    13081 tgaaacacaa tacccccaat cccccgaaaa cgtaaatagg tttcgttgaa aacgaagcta
    13141 aaaacttaat ggtaatcagg tccagaatga ccttgaatgt ccgattcaag ccgtatttag
    13201 tttttccgcg cgtacggggc cggtgattga caaccatctc ggtaacattc tcgccgcccc
    13261 agcgtgccag cgccgggatg aagcgatgca tttctccata caaacgtatg gatttgagcg
    13321 actcggcacg ataggccttc agagtgcatc cgtagtcgtg caggcggact ccagtgatcc
    13381 gcccgatgag ccagttggct gctcgcgaag gaagtgtgcg ggttacggcg ttgtccttgc
    13441 gatctttccg ccagccgctg acaatatcaa aaccttcttg gagtttgctg accagtcgag
    13501 ggatatctgc cggatcattt tgaccgtcgg cgtctaatgg aacaataatg ctgccgcgtg
    13561 catatttgaa gccggcgctg agtgcggcag tttgaccaaa gtttctccga aatcgaatca
    13621 agcgaacatt tgatgtattt ttctgtatat cagtaagaac aaaaaagctg ttatcggtgc
    13681 tgccgtcatc aacaaagaga acttcgtatg caaaatcctt taaggccgct gcgatttcag
    13741 catataaagg acgaagattt tcctcttcgt taaacaccgg tatcactatt gataacgcgg
    13801 gatgtcctgt ttctggcata accggctcct aaatacaagt actttcagca gttccgttaa
    13861 ttttacaggg acctgcggat tctgtaaaga cttatctgca atagaccgaa aacacagttg
    13921 tcaatccaaa gttttgtgct aaactgtaaa attattatcg gcttatttga acagaaatta
    13981 tgggcaaaaa gaatttaaaa aggaaacggg tatgaaatat cataacacta ccaaactttt
    14041 ttgtattctg tgtgctgtat tggctgtggg gagtttggcc ggatgccagc ggagtgaaac
    14101 gaaaactgca gaattgaaga cggaaccagc tcctaaaacg actaagccgg caccgcagac
    14161 acaggttggg cagacggctc gaccttcggt ttctccggcg atacagaccc ccaatgcacc
    14221 ttcaataaaa gtcgaaaatc cctccgttaa tttcggtcag gtaggtccaa attctgttca
    14281 caaagccgta tatgccttca gtaattcggg caatgcccct ttgtcagtaa ccaatgtcca
    14341 aagcacgtgc ggctgctctc tgccgacttt gattaaaaac gatcagcggt ttgcgattcc
    14401 attaagcgag ccggttttgt acgaacctgg ccagtcgggc aaagtagaag tgacttttac
    14461 agcaccttct acaaagggtt taacgacgaa gcatctctat atcatctcca atgaccctgt
    14521 tacaccgcga gccgaactgc ttataaatgc ggaagtaact gtaaaaatag cagttagccc
    14581 tgaaaatgcg gatttgcgct tcgataaacc caatgcagga atgccttccc ttattgtcaa
    14641 aagtttagat gaacagtctt tctctattcg ctcaatatca atacccaacg atgtgatgaa
    14701 gattgatttt gatcccggcg taaagaagaa agagtttgtt ctggagccgg tggttgcctt
    14761 ggataagctg cagcagtaca ccacaggggt tattcagatc acaacagacc atccccagtc
    14821 cggtacgctg gtagtgcact atgcagccca gcctaaatat gaggtctcac gtccgcggat
    14881 tatcctgcag aatgtcgaac cccaagttcc ggttattaag gatgttatca tccgaagcaa
    14941 ttacggtgag aaagtggaga tcgcttctgt tcaatcccgt cagggttata tgaccattga
    15001 aagccaagag ccggatggca ataacatcaa actaatggtc aaaattaccc cgcccgctca
    15061 aggtgcccaa cggtatatca cggatgagat gatacttacc ctcaaaaacg gtgaagaatt
    15121 atcgatccgc tgcagcggct ggtttagagt caactgattt tggccgaaaa catcttttag
    15181 gccaaagccg ccttttttcg gccgttttgt ttgggggaac tttcttggtc caaaattgtc
    15241 taaggaagta cgagaagggc tggattttgt ctatacaggt gttattaggc ctgaaaagta
    15301 agaaatcgtc tgtttcggag aagaaatgct taaaaccctt cgaataacca gtttaatcgc
    15361 cgtgatttta gcgatatgcg gggttattgc agttatattt tttggactga agggtgattc
    15421 cgaaataagg gcttacttag aaaaaccggg cattgtggag gagttaaagt caaaaatagg
    15481 cgacgcaaaa ggtaaaggag atgttgtgtc tccattggta gcacaggcaa agagttttgc
    15541 ccttcgtatt aatccgccgc ctccgccgga acctgtaaaa ccaaaaatag agcctaaacc
    15601 tgctccaaca ccggctcagg cgagtacaca gccggcacaa ccccccgttc ctaaagtgca
    15661 ggcctctgtt aaatttgact tgttggctac agtcagatat gagacagcac cagaaaagtc
    15721 gctggctttg gtttccaccg gcagtaagct cgaatggttt agacagggcg aaaaagtcgg
    15781 tcagcatgaa atcaaagaga ttcgtgacgg cagtgtgata tttacgcagg caggcggcaa
    15841 accgcaggaa atctttgtac cggcaaaaac tgaagtcaaa tcacttctta aaaatgagca
    15901 aaaggccgtt tcagcagtca gaggaccgac ggggatacag gatactccgg ctgctggtcc
    15961 caatggcatg caggaccagc cgggggttgc tgatgctgaa gctgtgcgtg cagcgcgtac
    16021 ccgtgagact gccgtttcaa cgcgaagcac agtagatgcc cgtatccagc gcgtacgttc
    16081 agccccgccg gtaccaactg tggaagagca aaaggcctca atcgagagtt ctatattggg
    16141 gattcatgag attatggccc gtcaggacga caatctctcc gaggaagaac ggcgtcagga
    16201 aaatcaaatg tgggcggaac tgcttcaagc tctcaatcag gaaaaggaaa atcttgaaaa
    16261 agctgcacag actaaaccgt ccgagtcagt gccggataat ctccccggcc gcagcgattc
    16321 ccaagaaaca actgccgcgg ctgcagaaaa aaatcaagat gcccctgctg tgccgcagcc
    16381 ggcggcatct tcttctgccc agactcctcc agcagaaggg ccggctgaac ccaaccagac
    16441 cggataaata agttttttac ccagcagaca aaaagatgtg actgaaaagc agaaataacg
    16501 agtaatgcag aggaaacaaa gatatgcagt cgtatagaga agcaggtatt ggacagcgga
    16561 aacagtggct ggcaggattg tgcatattgt tttggctgtc ggctgtcttg atgggacaat
    16621 ccgggggtgt gctgcgcagc cgggtttaca aactcaagca cataacaagc cagcaggcca
    16681 aagaactgct tgtccagctt aatatcggca aaagctacaa taccctgacc cgtgatgtgc
    16741 tgattgtaac cagcgatgtc ggtacagacc tgcttaaagc gacagagatt gttccttttc
    16801 tggatcagga gccgcctgtt caaattcgtg tgctgatgac cgctgcagat tcggcctgtc
    16861 tgcctactgt ggagatgctt gcggcccgat taaaaacaat tacttttggt acgatgaatg
    16921 aagctccggt cagaggagcg gctaaggcag ctattgtaga tactgtaaac ggcaaactgc
    16981 ttgcgattgg tacagttgat gttttaaatg aaatcgaagc agcggcggca gatttggggc
    17041 agcaatccca gacatcagca tctgctcaaa aagctgaatc ggccgcggca gaaaagccgc
    17101 ttgaaccgaa tctgccggca actctgatgc ctttagccga aacaaatctt ccggcacagg
    17161 aagaaccgaa tgccttgacg ccgcaaactt cagcgttttc gactgaaaag ccgttggagg
    17221 aggcagctcg tcagattttt gaaggagtgc cgcagctgtc tgatgttaat gcgccgtcgg
    17281 attcagcgcc ggcggccgcc gcagaaaagt caccggaaga ggattttctc agtgaagaat
    17341 taatgaaagc gctggctgat gcagaagaga cggcgcgtgc agcccagtca gctgctcaac
    17401 cggtacaaga aacggccccc gtcaaagaag cccaaacaca agtcccggaa ccaaatgctg
    17461 cccaagcaaa acctcaggaa gcaaaatccg agccaactca gccggaacag actgcaccgg
    17521 cagagccgat tgacccgatg aaggttttac aggcgctgat ggcccaagcc cagcaggaag
    17581 agaaggcttt ggtagaagag caagcacagg ctgaagctgc ccagcaggct gctgcttcca
    17641 gtcaggataa ggaacatcta gaaaagctgc agacagaatt ggcccagctt cgtcaaaaac
    17701 tggccgatct ggaggcctta gccggccaag cagccccttc gggcaatgcg gccgaggcga
    17761 aggttcaatc tgccggcagc gccccgatac ctgccgagat tgctgaaaaa gagctggaaa
    17821 ccgttattga tttaccccaa gaagttgagc tggaagcgct ggtggattta gtcggcaaac
    17881 agctgggact taactatatg tatgatccca caatactgaa aggtcagaag gtccagctga
    17941 aaattcacgg cggcaaaatc aaggtgaagg atacttatgc cctgctcgaa tctgttttgc
    18001 gattcaaggg atttattatg acccgccgcg gccagctggt caccatcatg cgggattcgg
    18061 aacttgccaa agcggaaccg gttctgcgag cgccggatga gccgattcag ccgggtgata
    18121 ttgtggtcag cagtctgttt acgctccagt atattgatgc ggccacggcg cagaatatgc
    18181 tcaagacgat gaatctgggc acaaacttca cgccgttgga aacaaacagc ctgattgtaa
    18241 ccgattacgc ttatcggatg gatcgtatcc gtcaggtatt gacgatggtg gatgtgcccg
    18301 ccgcagctaa ggaatatcga ttccggacgc tcaagtatat caagccttcg gatattgtag
    18361 gcaaactcaa agatctggca gggcaattgc agggtgttac gctgcaaatc agtgcagcag
    18421 cagccactcc cgccgctgct acgcaaaccc gtacagtaac gacccgcaat cctcaaaccg
    18481 gccaaatgga aacccgtcaa gtgccgattc cgaccccggc gactgctact gccacgcctg
    18541 ctccgcagac atctgcggca gttactggag cagccaagga tacagtattt atagatacgg
    18601 atgaccgcac caatcggatc ctcattgtcg gcagagcaga ccagattgca ttgattgagg
    18661 agttggttga tgtgctggat gtgccgcagt atgacctgaa gtatgtgcgg gaatatatca
    18721 tccagaatgt cgaggcaatc gaagtggtca atgtgcttaa tgaattggga ctggccaatg
    18781 tagctgtcgc gacacaggca gctggccgga cagctgcgcg aacggccgct cgcaccgtat
    18841 cgccccaagc acagcaggtc caggaggcgg ctgttgcggt gcagcgcaca ccaacagccg
    18901 gtacggctgc ctccggcgac cagccgtatg tgtcgattcg tcctgccacc aattcgctgc
    18961 ttgtcaacgg cactgctgaa cagcatcggg cgatcgagct ggttattgcc catgtggatg
    19021 tagtgcaaaa ggaccagcga actatccgcc aatatgaaat ccagtatgta gatactcaga
    19081 ctattatcga tgctctgacg gatttgggta ttatcgctcc gtcgaccagt gcatcgacag
    19141 gcgctgccgc acgcggcacc tcggctcgga cgtcttcttt aggccgtact acggctgttg
    19201 cgcaggctgt accgacagca gccgagcagg ctgcggcgcc catggttctg cccaccgccg
    19261 aaggcggctc ggaagtggaa ttgacagccg actacccgca gattacggtt ttgcagacga
    19321 ctaattcgct tctggtctat gccacgccgc gtcagcacga tgcgattgct ttggtaattg
    19381 cacatgctga ccgtcaattg gatgttacca ctacgccgta tgtagtttat gcgctggaaa
    19441 atcaagatcc tgtggaactg gccgaagtgc tgacaaaact gattcaggaa acggttgagg
    19501 aacgagccaa aacctcctcg cccgaagcca aaattcaaac ggcaccggca tcaggcactg
    19561 ctgccggcgg tggggcgcct ccaacgctgg aggaacagaa gattcgcatc atcccggatc
    19621 cgatgtccta ttcgctgatt gtctatgcca acaagcgcaa ccaacagtgg attggtgaac
    19681 tgatcaggga actggatgaa taccgcccgc aggtgctgct ggattgcacg ctggtggaaa
    19741 ttaccaagga tgagtcgttc aagtatgatt tggatgtcat atccaaaacc tataacgaac
    19801 tttcgctgag atccccttcg cccatatcga cgatttccgg taatttcagt cagcagcagt
    19861 acggcgaggc aaggtcaaaa agcggcgaat ttactggttt ttataacagc gatgcgatac
    19921 aggccctgct gacggctgtt cagaccaaaa gttacggacg agttatggcc cgtccgaaag
    19981 ttcttgtcaa tgacaaccag gaaggtgaaa tcaaaacaga aaataagaca tccattgctc
    20041 agcagaagag cattgttcag ccggctactg gtacatcgcc atcctatacc acaacagatg
    20101 taagcttcag cgagtattca gaaggcgtaa ttttgaaaat aaagccgcac atcagcaagg
    20161 gcgatatgct gcgtcttgaa atatcattaa gccgaaccga ttttacggaa cggcaggatg
    20221 ttacggtggc agggcaaacc tacccgcgtc cgccggatct tctttcgact gatataacga
    20281 cggtggccac tgtcccggac gggacaacca ttattctggg cggcctggaa ggcgtggatc
    20341 agcaaaaggc cagcacgaag gtgccgatta tgggcgatct gccgattgtg ggaggattat
    20401 tccgaggcgt taatgacaaa ggcagtcaaa gcaagctgta tgtttttgtc aaagccaata
    20461 ttctccgtcc cagcgatcaa atcgaaggtc tggacgatat ccgtcgcgtt tcctcaaaga
    20521 atcggcaggc ctttgaagag atggaaaaga aattccagga gcatcaggat tggccgggca
    20581 tcaaacccaa gccgatggag ccggttacag tcctggaaga agatgatttt gaacagctcc
    20641 ggcaggaact taaggaactc aatgaaaggt aaataatcgg caggcgggca tatcccgccg
    20701 cctgcggaaa atatgaaaga actattaatc caagcagccc agcgcagcgg ggtggtggat
    20761 gcccagctgc tgcgggattt ttttgaccag catgccgatg accagcggcg gatcgatcag
    20821 attttgctga cagcgccgac atttacagag gatatggtgc tgcggctgtt tgccgaagca
    20881 ctgggtattc cttttctcga tgagataaat ccctctgatg tgcctcgtga gtttattgag
    20941 caaattccgg ccccgtatgt tcagcatcat tatctcatcg gcatccggcc gcagggtgga
    21001 aatggcctga tacgggttat tgcagccaat ccgctcaata tgacagccgt cgataacatt
    21061 tccaaaatgc tgcgttgtcc ggtcgagatg ggattgagca cccgagcggc tattacagcg
    21121 gcgattgatg tggcttatga acagaaaaat acggtcatcg aagaagtagc cgaggaactg
    21181 gatgcccaga acctcgacca gctggccgat gaagtcagca gtgccgaaga cctgctggat
    21241 gtggtcaatc gaccgccggt gattcggctg gttaatgata ttctgtttcg cgccctgcag
    21301 atgcgggcca gcgatatcca tattcacccc tttgagacca aaattgtcgt gcgctaccgc
    21361 atcgacggca ttctgtacga taccctaacc ctcaaccgca acgttctgcc tctggtgatt
    21421 tcacgcatca aagtgatggc cggcatggat attgcagagc gccgaatgcc gcaggatggg
    21481 cggtgcagcg tccgcatcgg ccagcgtgaa atcgacctgc gtgtcagcac tgtgccgacc
    21541 agttatgggg aaagggctgt tctgcgtttg ctggataaaa gcaccggcat cctgaccctc
    21601 gagcagctcg gcctgtggca ggaagataaa gagatttttg ataagatgat taaccgcacg
    21661 catggggtca tttttgtaac gggaccaacc ggcagcggca aaagcacaac gctgtatgcc
    21721 tgcctgaacc gcctggattc gaccgtcaaa aatataatga cagtcgagga ccctattgaa
    21781 taccagctgg agggcatcag ccagatgcag gtggcgccga agaaggggat gaatttcgtc
    21841 aacgccctgc ggcatatcct gcggcaggat cccgatgtca ttatggtcgg cgaggttcgc
    21901 gatcaggaaa cagcggcgat ggcgattcaa tcgtcgctga ccgggcattt ggttttcagc
    21961 accctgcata ccaacgatgc ggccggtgct atcagccgcc tgctggattt cggggtcgaa
    22021 ccgtatttgg tcagctcctc gctggtctgt gtgctggcgc agcggcttgt gcggcgtatt
    22081 tgccccgact gcaagcaggc ctatgacccg cccgagcatg agctgcggga tttgggaatc
    22141 atacagaagg acaagacgcc tttttatatg ggagccggct gcgccaaatg ttttgatacg
    22201 ggctaccgcg gacgcacagg catctatgaa ttgatggtca tcaatgacga gatccgcgat
    22261 atgatttaca gccgccagac ggcaggcgcc atcaagcgca aggcccttga atacggaatg
    22321 cagactttgc ggatggacgg tgcccgaaaa gttctggctg gaacaacgac tttggcagag
    22381 gttctccggg ttacgcagtc ggataacata taaggattgt cagaagtaca attgcttatg
    22441 ccgagatttg aatatatcgc tatcaatgcc gataagaaaa cggaaaaggg caccattacc
    22501 gcagaaagtg ccttggcagc ccgcaagcac cttcgcagcc gcggactgca tccgatggac
    22561 gtaacgcaag tcagggtcga ggcggcccgc aggacattta aggatgtatt tgcccgcggc
    22621 gcccgccggg aagttgcctc ttttacaaag gaactgtcga cgatgctgaa tgccggcatc
    22681 aaattgaccg atgccttaag cgttttgacc cttcaggtca gtcacccgca cctgcgggca
    22741 gctgtaacgg aaatccgtga ccgtgtagta acgggcgaat cgtttgcgga tgccttggcc
    22801 gaatacgagc agtttttcga tgttatttac gtcagcatga tgcgggtggg cgaagccacc
    22861 ggcacgctgg gagacagtct ggcaacaatg gccggcttta tggataagcg ccagcggctc
    22921 gaagcgaaaa tgaccacggt gatgatgtat ccggccattc tgctggtttt ctgtgtaatt
    22981 gttgtgctgg ttattaccat tgctgtgatt cccaagataa ccgagcagct tgttaaaaca
    23041 ggccagcagc tgccgtggat tacttcggtg attatgaagc tttcggcact gctgacgagc
    23101 tggtgggtgc tggtgattat ggccggcatt gccggtcttg tgctgctgta taaacggctg
    23161 atgagaacac agcggggggc ttatctgcgg gataagctgc tgctggggct gccggtcgtg
    23221 ggcaagctga tccggcagga aattgtagcc cgctttaccg caacactggc tacgctgctt
    23281 ggttccggct tatccatggc tgaatcactg cgggtcgtca gccaagtgac cggcaatgtt
    23341 attatgatga atgccattaa gcaggcccgc gagcggattt tatccggtgc tgatatttcg
    23401 actcctttgc gggacagcgg tgttattgat ccggcaatag cccatatggt aactgtcggt
    23461 gaaaaaagcg gtgagctgga gcagatgctt cggctcatca gcgaaaatct ggaggcggat
    23521 tcagacctgc gaatcgaacg gttcagtcgg gtgattgagc cggtgattat catttttatg
    23581 gccggcatta tcggcatcat tgcctacgct acgctgctgc cgctgtttaa gtattcggta
    23641 acgcaatttt aacagaatcc agattacaga aatgacagaa atcttaattt tgaatagtga
    23701 ggtaactatg agacaggaat caaaaggatt tacgctggtt gaactgatgg cagtgctgct
    23761 gataattgct ctgctggcgg ggctggcggc taccaatttt atgggccaga ccgacaaggc
    23821 caaggtgatt accaccaaag caacaatgaa aacgctgcac aatgcggtta atatgttcaa
    23881 gctggatacg ggacgctatc ccagcgaaga tgtcgggttg attgaactga ttgagcagcc
    23941 gacggatgtc gagggctgga atccgcaggg ctatctggaa agcacaaccc tgcccaagga
    24001 tgcgtggaag aacgactttg tctatatgct caatcccgaa agcggcaagc cctttgtaat
    24061 aatcagctat ggagctgacg gcaaagaggg cggcgaagga tatgatgcgg acttgtacag
    24121 cacggatacg gactagacct ctgttttgta cggatagtcc ggccggtttt gtgctggttg
    24181 aaatgctgct ggttgtcggg ctgattgccc tgatgaccgg tgtggctgtt atcagtttcg
    24241 gggcgatgtg gggcaatttg cggtttaagc gtcaagccga gcagctactg acggcctttc
    24301 aaatggctca ggatgccgct gctcaaagcg accgccgcta tgcggttgtt ctggatttta
    24361 ctgagcaggc ctttattctt cgccaattta aatcactgga ccttcaggga atggatccgc
    24421 aggaagcgat tattcaaaca gtgcattttg atgattcact gactttggat tacgtgctgt
    24481 atgatgattt ggaagatacg cggcagtccg agaatgtgac cgaggcccgt tttctggccg
    24541 gccggtccgg ctggcagtac ggcggcaagg ttgttctgcg ggatgcggaa gggcggccat
    24601 ggaccatagt catccatcgt tttgccaagc cgccggagct gtttgaaggc gaagtcgaga
    24661 tctatctgcc gcaatatccg gaacaagtgc cgttttagtg aaaatgggaa cgtctggtct
    24721 attattggaa ataatgagaa aaacaagcaa cataacgcca agcggtttta ctatagcaga
    24781 agtggctgca gcactggtgc tgctgtcgct gatattatcc tcggtgctgg tactgatgaa
    24841 ccggtatgcc gcggcagtag ttgatttgca gcttcaccag caggcctttg aactggcgcg
    24901 agccaatatg gagcggctgc tgtccgaacc gaaactgtcg gacataagcg actacggcac
    24961 cagcgatacc aatcctgaat tggaatggca gacggttgtt gagccattct atgagcctgt
    25021 aaccaatcag atgtggattc gggctgtctg ctcagcgggg tatatggata ccaagggaca
    25081 gtatcagaat gtggagttgg aacactggat tacaaatctg acagctgagc aggtcaagca
    25141 gattctagcc cagcagaaag ccgaagacgc ctatttggag cttctgcagg aaggtcagct
    25201 gacggcaatt caggaaacaa cccgtgccta tctggagcag atgggcttgg atgtggaagc
    25261 atacgagaag ctgatggctc agcagcgtcg gaaaaagctg gaatatcttt cccgcaatgg
    25321 atttgaagga taccaaaact ttcttggaca gcttgaagaa gaggaaaacg cctttctcga
    25381 aaagattgga atgaacttcg acgggtataa tgaatttgca cggacttatg tacccagaac
    25441 cgaagcaagc gaatcttttt ccgtcgattt gcctcaagaa ggatcggaaa cgatgcagga
    25501 tacggcaaaa gatgcatcgg ataccgcccg cccggcggaa aggtctgatc cgagccgcaa
    25561 gccggcatcc gacggcggat ttaactggga caatgtgccc cccgaactgg tcccgctgat
    25621 tgaacaattg ctgggaatta aaaagaacgg atgaaaaggt ctaagcaaaa tgcgtttaca
    25681 ctggttgaga cgctgattgt gctgatgctg gcggccttga ttgtcacagc agtgctgggc
    25741 atttatcagc aggttcgcgg tgcagcggtt gcgattctgg agcggatgga gaagaatcgc
    25801 ctccagacgg agattcttca gaaaattgcc gaagatatcg accggctggc tgcccccggt
    25861 tttgatgctg ctattcggtt tcgcagcaag ctggataacg gattccgctc atcgcagctt
    25921 gttctcgaaa acagttatta cggcagcacc aaccagaaac agacctatga acggattatc
    25981 tggcagacgt cgtatgatcc tcaaggcaag actctgattt tatatcggat gcacgacgga
    26041 ctaaacgtag aggataaggt tcttgaaaaa gatgctgcct catcaccgag tgctcagatg
    26101 tttattcccg ttgccgccgg ggtaacctat tttgatattc aggcccagca aggtgagaac
    26161 atcttggggg tttggctgtc tgataccctg cccaaagccg tacgaatcgg catctcattt
    26221 gccccgctgc agcaattagc ggatggaagc atcggtgtgc cggaagaaaa tatcctctat
    26281 cggacagtag ctgttgaccg cacacgggcg ataccgtatg agtttgtcag aaaaaagctt
    26341 gaactgccga tggagcagga tcccaatgaa ctgctcaacg aagccgatcc aaataccgtt
    26401 tccggtaaac agcacagtgg tgtgaaaaaa tgaaatcagg ccgaaaaaca acatgttttt
    26461 ctttgacggc ggacaggtcc agcggatttg cctttctggt aacactgata gtattggttg
    26521 tgctggcttc gctggctgcc ggattatcgg tgcggctgac catggccaag cggcgccagc
    26581 agtatatggt tgattatcag cgagcccgct atggggccga ctctgcgatg aaatatattt
    26641 tatctgtgtt gccggccaaa aatttccccc tgcagaaccg caaggataag ccggattttt
    26701 ctgacttata ctggatggat cagcaggaat acggccggtt tattgcggac tgggcagctg
    26761 acgccacaga tgagcagatt caagctgtgc ttaagccggg agcgtcgctt cggcctgctg
    26821 aacagtccaa tcccaaagac ctgattgccc agctgctggc cttatttgga gcagaggaga
    26881 aattgaatga gacagagcaa tccaatagga tagatgcaaa tattccagat tccactattt
    26941 cttcagctgc catggaagga ttcgaagtgg tcgaactgga tcccaatgat gtccaagtgc
    27001 cggggccata tgggccgcct tggccttatg taattgaacc cattgagctg gaaatggggc
    27061 cttgccgtat tactattgtt attgaagatg aaaatgccaa gatgccgcta agctggctgg
    27121 tgaccagctc ggcacaggcc aacaagcgtg cggaaaatgc acttaagaca ttctgcgaat
    27181 ggatggcttg ggataaacag agtcttgctc ggctgcagaa tattatcgag acggcgatga
    27241 aggaagtggc caaaaagaaa atgtttaccc tcaatcccag tccaattctg ctcaggcctg
    27301 tttcccaaac gccccagaca cctgctgcaa ccccccagac acaacgagca accacccgga
    27361 tgacgacttc tccagccgcg gcccaagcag ccgcccagcc cgctgcccag acgcggcccc
    27421 cgtctgccca cgcaacggat tttgccaaac tgtttcacag ttccctgctg gaccttgaag
    27481 ccttggctga gccgctgccg aataccggcc agcgtcagga aagtccgctg aaatatttat
    27541 ccttgtgggg agcacagcgg gttaatatca atactgcacc gcggcatgtg ctggaggctg
    27601 cgtttacact tgccattgat ccgctgactg ccagccagct ggcccagtcg gttatcgttc
    27661 agagaaggga aaaaccgttt cggacggcgg ccgaattaag agaacttggc cgtttggatt
    27721 cagaaacaat gactgttctg cagaattata taacgaccac cagtacattt tttcaggtgc
    27781 gggttatcag ccgaagcggc aatgcccgtg ctgcggcagt ggctacagtt atcaaggaag
    27841 gcagacagat ggaacggctg gccgttatgt acgagtaacc taacaccgat ataaaaccgg
    27901 agtcaataac atggatgaaa aaggaagctt aggactttat atttcgcggg gtaaagcgac
    27961 agcggtatgg gtttctgcag atacccagcc atccgttaag ctgctgttcc atgtggtgcc
    28021 ccagcccggt gaatcacaga gtatagccct gcaggccgcc cggactgcaa tgcagcaggc
    28081 agcggcgttc ggcgaggttt tcgctgcgat cgattgcggc tgctatacgc aatatttcct
    28141 gcattcggaa ttcgaagatt atgcgcaggt agccggcacg attaagtatg atgccgaaga
    28201 ggctgccgct gcggatgcag cgaatttggc ggttgccttt gatattaccg gacgggattc
    28261 ttttggttca aaggttacgg tttatgcggc tgacagacag tatctgacag atattctgat
    28321 ggatttgcag gagggcggtt tggatccgac gtatatggag ccggatgctg tttgtctggg
    28381 acgcgccctt tgtcagttga cgcctataga cgagcaagcc gatgcgctgt ttgtgatttt
    28441 gtcggagccg aattgttatt taattcgccc gaagcaggat tatgctccta cggtacgcac
    28501 atttctggct ggacaaggtc aggatataac ggcagtgctg gcacgggaag tagtgctttt
    28561 ggccgcagcg gaagaggccc aaaatccgct gacaacactg gtgcttatcg ggcgaacaga
    28621 tgcaattgat aaaacccgcc tgcaccagcg caccggtttg gaaataaaaa cagaaacgcc
    28681 cgaaaaatcc cttggcattg cggatgccga aagggccggc tgctatgaac tgctggccgc
    28741 ctatggagca gcactggcag gccaaacgcg cggtcataaa gctgattttc gccaggactt
    28801 tatgccctat caaggccgcc gcaaaatgct tgtcggcagt ttgcggacca tcagcatttc
    28861 tctgacggta ctgctgacgg cggttgcttt gttctttcag ctgaaggcct ttcagatgcg
    28921 ccgctatgta agcagaatga gcgaaaaaac tcttctggaa cataaggcgg ttacctatgg
    28981 caaactgcct ccgcggggaa tgacgccttc ttccaatctg cgccgggaat acacccgcgc
    29041 caaagagatg gaagaaggac tgggtcccgg ggatgaaaaa tcggttcctg ccaaactaac
    29101 atttttcttt gaagccgtca gcaaaacacc gcccggagtt gatattaata ttcagcagat
    29161 taccattacc gagcgttcca tgaaggtcaa gggcgatacc aataacaagg ccggcacaat
    29221 ggctttgttc aatgagatta acaaacatcc gaggatatcc ctgacatcgg agagaaccag
    29281 ctcaagtccc gatggtcgcg atacatttga aattacgatt gaacccaaga aatgacgaga
    29341 tcgaccaatg aagcagatgc ttaaaaatcc agcattttat tatatcttat tgccggttct
    29401 ggcgggtctt tggttgctga cggccggatt tattttttat ccccggtcag tagccgcctg
    29461 ggaggacagc cggaaagaat acgaaagcag ccgggagtgg atagaaaaat tggtaatcct
    29521 tcagcccaaa cggctggcct ttcaggtgga tccgtctgcc aagtcggaag aatttgattt
    29581 tactcggaca atttacgaat ttgctcaggt tttttccatt tctcccaata actatacatc
    29641 caatgtgcga ggcctagtca agcgggccgg gcgaccgagc cgttcagcag caattacgat
    29701 taaaagcatc gatattgaac ggcttgcaca gtttctttct gccatgctgc tccgctggcc
    29761 ggaattgaat tgcgaagtat taagtttgga aaaaaacaag gccggcaaaa atgactggaa
    29821 ggtcgaactg actttgacct actattatta gcaagactgg aaagcggtaa atcatcctct
    29881 tgaataggaa cttttcaggg catttcttga cgtccggcac gttttctgcg atactttata
    29941 attgatgagt aaaaaagttg ctattttggg ttcgaccggc tctatcgggc gcaatgcgct
    30001 tcgtgttctt gaggcgctgc agcctgatta tgaagttatt ggactgagtg cccataacaa
    30061 cattcgtctg ctggccgagc aggcgtcgaa attcaatccc cgctatattg ccattacgga
    30121 caatcccggc caattggctc taaagcagat tttttccggc tttcaagggc agattctaac
    30181 gggacctgag agtttggtcg aattagcttc gctggatgag gcagatatcg ttctttgtgc
    30241 ggttgtgggc gcagccggac ttccggcggc gttagccgct gccagattag gcaaaactct
    30301 ggcgatcgcc aataaagaac cactggttat cgccggtgaa ctgctgaccg aagccgcccg
    30361 ccaaagcggc gctgttctgc tgccgattga cagtgagcat tctgcgattt ttcaggctat
    30421 gcaggccggt cagcccgacg agattcgccg gattatattg actgcttccg gcgggccctt
    30481 tcgccaaacc cctttggagc agataaaaaa tgctaccatc cagcaggctc ttgctcaccc
    30541 gacttggtca atggggccta aaattacaat cgattctgcg acgatgatga ataaggcatt
    30601 agaggtcatc gaagccgtat ggctgtttgg tgtgccggtt gagaagattg aagtgcttgt
    30661 ccatcctgaa tcagttgtcc attcaatggt tgagtttatt gacgggtcca tcattgccca
    30721 gcttggtacg ccggatatga aagtgccgat tcagtatgct ctcacatggc ctaagcgcat
    30781 gcgtggaatc ggggaccgtc tgcagctaag tagtttgggg cagctgcatt tcgagacgcc
    30841 gaatctggag cgtttcaggg cgctagagct gggttttgaa gtagcgcgta ccgccggctc
    30901 ggcgccggtg gtctttaacg ccgccaatga agcagcagtg gcagcatttc tggatgggcg
    30961 aattcatttt ggacaaattg tagaactgat tgaagactgt cttctgtccc attctgtaca
    31021 gaggcatttg acactggacg aattgctgtc cattgacggc tgggcacggc ggtttgtggc
    31081 tgaagcactg gtgcggcaga cggcagcagg ttaacaggaa acattttagg ggatttcatg
    31141 acaaataagc aaacgacatc acaggttgca gaaaaagggg ggaaaaatac cccgcagata
    31201 atcgtctttg tgctggctgc agcagccgtt gttatttggg gaatcaataa tccatctgtt
    31261 gccttgcgca ttctggcagt gatgctcgga ttcggcggca tcgtgatgat tcacgagttt
    31321 gggcatttta tcgttgccaa attaggcggc atcaaggttg aagcattctc catcggcttt
    31381 ccacctgttg ttgtaggaat acgcaagctt aaaaagggct ggcggatacg tctgctgccg
    31441 aaaatcggcc agccgcagca tctggaagaa ggcgataacg aaaccgaata ccaaatcggt
    31501 ctgatacccg tcggcggatt tgtaaagatg ctgggccaat cggattccgg tgccgccgaa
    31561 gccaccgatg acccccgttc gtatgccaac cgtcctatat ggattcgtat tgccacagtg
    31621 gcggcaggag tggtattcaa tgcgattggc gctgtaatta tttttatcat cttatttatg
    31681 aacggcatca atctgaaacc tgctatggtg ggacaggtgg tgcccaattc tcctgctcaa
    31741 gaagccggtc ttcgtcccgg tgatgagatt attcaggtag acggagaacg ttttgttgat
    31801 tttgaagccg tattgctggc acctgtgctg tcggtgccgg gtgagccggt tacttttgtg
    31861 gtgcgggaag cagacagcgg ccgggagagg aaagttagaa ttgttgcaga aaagcaggcc
    31921 ggtgattcat ccaatttgcg caaaatggga attgctccgc tgacaaccct gacggttgaa
    31981 cctcagattg cccgttcgcc tgaactggtc gatgagatct ataaactgac aggtctctat
    32041 ccgggtgatc aggtcaaagc tgtgaatgga cagccggttg accatccttg ggattttgct
    32101 cgaaaggtat cagggatttt tgattcgaca gtaaggctga cgatagcgag gcggtggcag
    32161 tcagccgacg gttctgaaat gctggtcgat gtggattttc caatgcaggt agggcctgtg
    32221 gtggaaaact tccgtgatga atttgatctg gcacacttta gccggctggt tccgcgtctg
    32281 aaaatagaag ctgtcatgga gccgactcac gccgatactc tgatacgatg gtttcaaacg
    32341 gcgatacttc gccgcccgct tgagccggcg gcctgggatc tgctcaaagc gggagatatt
    32401 ctgcttaagg tggctgatgt tgaatatccc aattacaaac agcttcggga attgacaacg
    32461 gcttatgagg gcaaaccgct tcccattaca gtccttcgca gcggccagca gggcgatact
    32521 ttgccgatag aaatagttgt aactccaaaa gcccgaatcg gcagcaaacg gattatgatt
    32581 ggctttgcgg cgggtcttga tttagacaat ccggtggtgg ggcaggtttt gtcccgaacc
    32641 gatcaggcag aagacctgcc ggccgttcct gctggagctg tcattactgc aatcaacggc
    32701 cgggcggtag agaattttta tcagattgca gcgatgctgc aggacaacgc tggccgaagc
    32761 gtcagcatcg attatcagta caatggtcag ttcggcagaa cgaacctgtc ggtggatgcc
    32821 tatgagcctg ttcatgcgca ggcg
//
LOCUS       DAOTBO010000023        32432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1129_length_32432_cov_5.681811, whole genome shotgun sequence.
ACCESSION   DAOTBO010000023 DAOTBO010000000
VERSION     DAOTBO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 32432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32432
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_32432_cov_5.681811"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..592)
                     /locus_tag="PLE88_04585"
     CDS             complement(317..592)
                     /locus_tag="PLE88_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75779.1"
                     /translation="MYRRKKSQTITDVDKAYGNDTFLHEMLHALVYVWEEKYGSQAHV
                     GHGSKETKEEFEKCRELLVNAIYQPAEQTMPPNNEDNEIEHEVDDEE"
     gene            complement(562..873)
                     /locus_tag="PLE88_04590"
     CDS             complement(562..873)
                     /locus_tag="PLE88_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75780.1"
                     /translation="MSDNIHCSDEINFYPPTHENLLQNYNKREAGVVRNLIAQYKSKK
                     DTSEKAITDVARHIFLMYHLDPRLIGKASYGGDKSKAKVTSNKKIKSIDICIAEKKAK
                     Q"
     gene            complement(1100..1759)
                     /locus_tag="PLE88_04595"
                     /pseudo
     CDS             complement(1100..1759)
                     /locus_tag="PLE88_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            1995..2633
                     /locus_tag="PLE88_04600"
     CDS             1995..2633
                     /locus_tag="PLE88_04600"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOM75781.1"
                     /translation="MFTGIIEAVGEIRQVLPQGQQMQLVIGLGRAGEGVRAGDSIAVN
                     GVCLTAAKVEGGCAVCDVSEETLKRSTLSRLKVGSKVNLERAMSAQGRFGGHFVQGHV
                     DGTGRIAEIRRQGEFAVFRFEVSTDILNQIVLKGSVAIDGISLTVARVDAAGFEVALI
                     PTTIKETCWMTAKVGDAVNIETDILVKIIQKQLSAILPQQTITVEKLKDYGF"
     gene            2630..3676
                     /gene="pdxA"
                     /locus_tag="PLE88_04605"
     CDS             2630..3676
                     /gene="pdxA"
                     /locus_tag="PLE88_04605"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOM75782.1"
                     /translation="MNGTINNLLGNNTVIGITMGDPGGIGPEIVVKALADPMIRRLAK
                     FVIFGLDEQLEYAADLAEVEPFWLRHPHEKISRNYPRQVVVADYDEYSVPAWVHASTR
                     VSGEASMRFCLDAIDAARDGLIDAIVTAPISKTSWHLAESPWTGHTEMLADRCKSPRK
                     VMMFVAGPLKLALATIHEPLFEIRHKFTIGCVFEPIDLLDMALKQYFGIAKPHIAVAG
                     LNPHAGEDGQFGDEERRIISPAILLAQEAGINCTGPYPADTLFYHAVHGAYDGVVAMY
                     HDQGLIPIKMAAFDQAVNVTLGLPIIRTSPGHGTAFDIAGKGIANPNAMKEAIRVAVQ
                     MAKTSQKNKIQTGK"
     gene            3827..4711
                     /gene="rsmA"
                     /locus_tag="PLE88_04610"
     CDS             3827..4711
                     /gene="rsmA"
                     /locus_tag="PLE88_04610"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOM75783.1"
                     /translation="MQTKQDIERLLAGAGVAPNRRLGQNFLIDLNLMRLLLDAAQMHQ
                     QDVVLEVGTGTGSLTQAIAPRCGQVITVEYDSGLAKIARSQLAAVPNVTLFNMDVLEN
                     KNAIHHEILQALQTAQDSLGGRLLLVANLPYSVASCVMANLICGPVTAQGMYVTVQKE
                     VAERMAAGPNHEEYGVLSILMAAVGSVHILRKLPPSVFWPRPQVDSAMVSFVRDEKKA
                     AQIHDMHTFQEVIGLFMTHRRKMLKACVRFAPASLAHIRHWPDIFKEAFVDPHSRPEE
                     LSAAQYISVANLCYEQRR"
     gene            4756..5868
                     /locus_tag="PLE88_04615"
     CDS             4756..5868
                     /locus_tag="PLE88_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOM75784.1"
                     /translation="MQTRRQTKWKAAICFGWVLVLAAGLSAQNRTFLTGGDISMLSRL
                     EETGVVFKDDGQPKDLIKLMGDYGANCFRLRLFVEPSGRGGVIQDVPYTINLAKRIKA
                     AGAVFLLDFHYSDTWADPSHQSKPKAWENLSFADLTRQVQLYSADVIRQLTQAGVQPD
                     MVQIGNEIAPGFLWPDGKLSGKEPEAEWQRFTTLLKAGIAGVKQADPNETIQIVIHIE
                     CGGDAKKTDWFFSHLDQYGVAYDIIGLSYYPWWHGTLADLRTNLTQTAERFKKPVFVV
                     ETAYFNQPFDPGRGTFKDNLAWEKSPAGQQAFLCDVVQSVRDVPDGMGMGVLWWYPES
                     VPIRQSGGWNNGATALFDSNGRPLPALSCFQPPAGK"
     gene            complement(5876..6739)
                     /locus_tag="PLE88_04620"
     CDS             complement(5876..6739)
                     /locus_tag="PLE88_04620"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOM75785.1"
                     /translation="MRTIAYNQVVQSVETLCIDCAFQLPPDVAQALRQAAGRETNPRA
                     REILEQLIENARIAAEERIPLCQDTGLAVVFVQQGADVQIVPPDDKPAATIVDAICQG
                     VAAGYEKGFLRKSVVAEPLNRRQNTQTNTPPVIHYSVVPGQTVHISLMAKGGGCENKS
                     CLRMFNPTDSKEAVIDWIVQAVKQAGASACPPFVVGVGLGGNFELSCLLAKKSLLRKI
                     GEANPDPFYAQMEQDLLAAVNASGLGPMGLGGDTTALAVCIETAPCHIASLPAAVNIE
                     CHSHRHKSITI"
     gene            complement(6739..8919)
                     /locus_tag="PLE88_04625"
     CDS             complement(6739..8919)
                     /locus_tag="PLE88_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75786.1"
                     /translation="MKLKIAAVLGLFLTAGAAGWAVTSAIIRHQTAADFGKGKTDKVI
                     ADSAGTLRLARQSREIHCDGLLNDVWSIHTILAEPDGTLFLGTGPNAAVIRYDGRKAE
                     QVYPRDSADPNQSADSTIRNEHIFALARDVAGRLLIGVSGTKGRLVRLSGQAETVFTD
                     PRVQYIFAIAADADNNIYLATGPEGLIFRLDPFCQNPEVIWDAKDKNILSLAIKDHIL
                     YAGADQRGLIYKIDTVQKQTSVLYDAEQDEIPALWIDSDGNVYAAATSAQAAMLQLQT
                     PAAPQRRAPGRPDVDNDKRPVPLEGSLNTPNSSETKEEKKEAAPAPQPPAPPAAKAAG
                     HIYKITPEGFVTDMFTEMAVLYSLHCADGKLWVGTGNKGQLFAIDPAADEQALVFEDK
                     ISTQITSALPVNGVIYLGLSNPARLIQLDSAVAPTGTFESALLDAGQPARWGKLQIEA
                     DIPKGGRITMACRSGNVQDPNDATFSPWSPETVIDGPVELACPVARFCQYRLTLTAGS
                     DNASPVVRQVTAASVIPNLAPTVAAVKAEPSRDPKKVGIIDISFSARDDNRDQLEYTL
                     EFRKLGWTVWIPMKDQLDQNRFEWDGRTVEDGRYEIRVTASDRKSNTPEAALTGSRIS
                     EPVVIDNTAPQITDAQLRIEGTSIVLQMTVTDALSVLGRVQYTVDSNEKWTAGLPDDG
                     VCDTLTERFTMRIDDLKSGQHIVAVSVSDDLGNTLYKTYEAAIP"
     gene            complement(8944..10731)
                     /locus_tag="PLE88_04630"
     CDS             complement(8944..10731)
                     /locus_tag="PLE88_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOM75787.1"
                     /translation="MRYSGTGKAIFCILWMVLIGQMGRAENLPPADCISLDEVRPDMD
                     AYCLTVFSGSKVEKFPLKVLSVVRNQRPGQDMILVLGTDERFNQAGAVHGCSGSPVYI
                     DGRLAGALAAGWDGSLDSLYLVRPIKDMLEVGTAQGTKVSASTTAAFDFSQPLDLEVY
                     YRQYMERLQERRTDNDLLLPLSTSLPAEVCQQYSTVLRRIGLMPAAAGPTVLTATAED
                     AGNFEPGSVLAVVLCGGDISLSGIGTATAIVGDQIYGFGHSLNGLGPVNLPIAAGVVH
                     TVVASRSYSFKLSSPGPILGTLEFDQNCAVRGTIGKIPRTIPLQIEVSRFNDPRQRVY
                     NCFLADDRGMTPSILQVVLSGAAQMQGPLPSEHTVRYAGQIVLEDGQPLLIDNVSSGR
                     QLSDVVSDAASAVDLLMNNPFGPVPIRSINARIEIDPVSIAASIWKVNVSNARVRPGQ
                     TITAEVTLRSFRAPEKTAAIELKVPETLDAGKYKIHIFGAAGYQAFVSKAAPYKFRAY
                     DLPTLKTGLEGLFKYRRDRLYAVMPIPATGLVIRQHELGQLPPTKVLLMQDAKRLQPL
                     EPYNAWAEGSTSLDAVVDGTAEIEITVEK"
     gene            10933..11730
                     /locus_tag="PLE88_04635"
     CDS             10933..11730
                     /locus_tag="PLE88_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75788.1"
                     /translation="MDAGHRHELKTNELAQQLGQLPKLAKENANFIIGTALIVIGLIT
                     WPVFSRMRLQKDMAAQSSITQSIQMLDKDVYDILQAQDGDAQNQALGTLLVNADTLLE
                     KAEKIDNPNLAALARIKAAQAIRTELHLRKDVSPEMLENQIQKAKSAYEQALESAKTP
                     TLKAMAKLGLGLCAEELGQTAQAGDIYKEIVSDKTFEATVFPKQAQQRLDSLEDNAQT
                     FTFAPAPAEAPAPAAPVQTSQTEPLAVIEAAPEPAVPATPEQTSSQP"
     gene            11734..12828
                     /locus_tag="PLE88_04640"
     CDS             11734..12828
                     /locus_tag="PLE88_04640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOM75789.1"
                     /translation="MSDLNDIPASPVDDSDDEALDNQVIELDRNLTDEETTQLAGRPL
                     RFRVGSNLKFNRLDKYLCGRFSQFSRTRLQALIREQGVNVNGRPAKPSHKLQAGDIIE
                     LILPARELRELTAENIPLHILYEDDDILVLNKQANLIVHPARGYKSGTLVNGLVYHFQ
                     HLSSCPDDIRPGIVHRLDRNTTGCLVVAKNDTAHWKLSRQFADRTTKKTYLAIVHGTP
                     ELHSDCIKQPLGMHPVIREKFSIRPDIGKEAVTFYEVLEAFRGFSLLKLDLKTGRTHQ
                     IRVHLSYIKHPIVADDMYGGKLVYPWQIENRQPAAEEPLLARCALHAWKLELTHPVTQ
                     QRKTFEAPLPDDMQRFLDALRTYRSLPAKA"
     gene            complement(12825..13562)
                     /locus_tag="PLE88_04645"
     CDS             complement(12825..13562)
                     /locus_tag="PLE88_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOM75790.1"
                     /translation="MGFLYGCTAGRINIAALSPEDRQHVESIIDRLEPMIRQRRIQED
                     LAALTFEDLFVPLDKKQKSFLRRFLNLDVRRLQLTIPYQGIPTQTPDFIALRGQTITR
                     TDGRTEELPVQFLPIPVYEAFSAMVSQMKQDIGAGIYVESGYRPAAYQLYLFLVYLKN
                     HEYSVLKTARFAAWPGYSEHGWPQHQAIDIITEQGINGQDNPEEMTATKQYQWMLENA
                     GRFGFEMSYPEGSAMAFEPWHWRYKGS"
     gene            13759..>14815
                     /locus_tag="PLE88_04650"
     CDS             13759..>14815
                     /locus_tag="PLE88_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75791.1"
                     /translation="MVKEADNLTPAMKQFRHFKSKYPDAILFFRMGDFYETFFEDAHI
                     VSRVLGLVLTSRGKSGDTPIPLAGLPYHAVDGYLKKMLQAGYKVAVCEQVEDPKLAKG
                     VVKRDVIRIVTPGTLTDEMLLEAKEDNYLAAVWLGTKQAALSWVDISTGHFFIRSLAD
                     KHILDELLRLGVREVLLAECRGQLFEAEQKKLVHQIRQLTGAVITERPGWYFDPFGAK
                     ENLLKHFGVSTLEGFGISEDFEGICAAGAILEYLDETQKTALNHITSLRLVKPSDYLQ
                     IDPVSLRSLEVVRTIRGESSKGTLLDCIDQTVTGMGSRMLRSWVCMPLRCTAEITARQ
                     DAIEELVNNDALCTQIRK"
     assembly_gap    14816..14825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14826..16373
                     /gene="mutS"
                     /locus_tag="PLE88_04655"
     CDS             <14826..16373
                     /gene="mutS"
                     /locus_tag="PLE88_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOM75792.1"
                     /translation="IADLERIAARISTYRTSPKDLVALGITLRTIPQIRQLLSDCSAS
                     MLQSLCGRCDSMDELADLLEQAIEPEAPSHLRDGGVIRSGFSDQLDNLRSLSKDGQSW
                     LAEYQQRQIQRTGIDKLKIGFNNVFGYYIEVSHAHADKVPADYVRKQTIKNAERYITD
                     ELKQYEEQALGAREKALELECRLFEQVRQETTRYIRRLQELAHVLAVCDCLAALAYLA
                     RQNHYIRPKVTDGRELVIIEGKHPVLAQMLGAEFVPNDIILSEDGKDAAIITGPNMAG
                     KSTYIRQTALLVLLAQTGSFIPAKKAHIGVADRIFTRVGASDELTRGQSTFMVEMTET
                     ANILNNATDRSLVILDEVGRGTSTYDGLALAWAITEHLANVIKCRTLFATHYHELTEL
                     GQLLANVKNLNVAVREWAGEVVFLHKIIPGGTDKSYGIHVAKLAGIPKSILTRSSEIL
                     KELENTFAKEAAGEHLSKNKTADEQLLFVQKHKSVLDKLAGLNVNKLTPIEAINLLAE
                     IQQEISR"
     gene            16539..16874
                     /locus_tag="PLE88_04660"
     CDS             16539..16874
                     /locus_tag="PLE88_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75793.1"
                     /translation="MIDKLQKVVNSLLGLCEEGGAGVSEEDIVSPEYHKYEPLGIMIL
                     KMQDGVLEKRYFLRMEKWLLCDPAAVEYYIDFQMLTALLHEHFNKSRLEKIADSFREI
                     ITGKAAANS"
     gene            complement(16988..18592)
                     /locus_tag="PLE88_04665"
     CDS             complement(16988..18592)
                     /locus_tag="PLE88_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75794.1"
                     /translation="MDKNAVKRFFKGTYTVWKCFLWGLRWLSIVAFGVLFFTGIYFEL
                     PWKVLVCLAVIPAVGIFVPRKIQPWVWMGLTLFLLVVWGWVNLPEHNSSRWRVYQYDK
                     ALAEIERDYLPEGMENAADLYQPILDTYSETIFYYSFSSPQEDALTLSQPWDPAAYPQ
                     LDAWMLSLQPAIERLSAAARVEQCRFEIPYNLAALKPQMERINRLKGWARLLLRAANR
                     DLYLGHQEQALEKMLAVVNLSQHLYQQQTLFDQAGAFYVELMGARALETYLVRYCENP
                     DWAVKIEDVFEDLNPRWTRNWPHILQREKLLTKNIAALLYEVNEAGRIRISHNAAAAL
                     QEGLGYPARRLFLNQHQINRLAVIGLWLSLPMSPQRLAQLIDKRFDYYSLQVQKGQQL
                     PNFSIKYVWTLGLNAQSIIDWLAMQQVGYFWALDGQYRRHQAIVRQMHLFSGLKRYFL
                     QHGQWPERLEHIERDSDELLIDPLTSRPFVYERLDSEFRLYSLGPNGVDDGGINHPKL
                     KKDDILLWPRKTTQQDNVRTSKPSNG"
     gene            complement(18684..19151)
                     /locus_tag="PLE88_04670"
     CDS             complement(18684..19151)
                     /locus_tag="PLE88_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOM75795.1"
                     /translation="MIADHLNNASLYNGLDRRIAAGLELLKKDTVHQSLPGRYEIEGD
                     ALFYIVDEYQSKPFEQGRPEIHQQYLDIQYIASGTECIGYAVLNGLDQEQPYDSRKDV
                     AFYKHCPQMSRIVLKAGMFAVFWPNEVHMPGCSAGVGQRIKKIVVKVRMERQV"
     gene            complement(19185..20666)
                     /locus_tag="PLE88_04675"
     CDS             complement(19185..20666)
                     /locus_tag="PLE88_04675"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOM75796.1"
                     /translation="MAKLAKLDDIVSLCKRRGFIFQSSEIYGGLASCYDYGPLGAELK
                     RNVRNAWWKAVVQMRDDVVGLDCSILMHPMVWKASGHADKFADLVTVCKKCNTRSRVD
                     HLQDKSDGGSDQEHTEHINIEIEAGKPHDLSRKVCPACGAVGQFSQPMAFKLMFETQM
                     GANVDDQMTLFLRPETAQGIFVNFRNVLDSTRVKVPFGIGQIGKSFRNEVTTKAFIFR
                     TREFEQAELEFFCKPGTDEQWYDHWKQQRFSWYLNLGIKKENLRFREHEKDELAHYAK
                     ACVDIEYKFPFGQGDWQELEGIANRTDYDLRQHQRGMRTMNDWFEQKGDLTKITLKEE
                     DPDYVKGPLSYFDEETRERYVPYVIEPSAGIDRSTLAFLVDAYDEDEVNGEPRSLLRF
                     HPHLAPIKAAVFPLVKKDGMPEIARKLYDDLKGSFACFYDEKGAVGRRYRRQDEAGTP
                     FCLTVDGQTKEDNTVTIRERDSMVQIRVSLDQVKACLHDKLNL"
     gene            20860..21036
                     /locus_tag="PLE88_04680"
     CDS             20860..21036
                     /locus_tag="PLE88_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75797.1"
                     /translation="MAHIGIPGGIEWLTIFFIGFFGIGMKLAFMVFVIVFLIKINSSI
                     ESVKKKLDQLEQKR"
     gene            complement(21137..22012)
                     /gene="prmC"
                     /locus_tag="PLE88_04685"
     CDS             complement(21137..22012)
                     /gene="prmC"
                     /locus_tag="PLE88_04685"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOM75798.1"
                     /translation="MAMEVWTIKKLLEWVSGYLEQKGVDSPRLSAELLLCYVLGLERI
                     QLYILYDRVVEKPQLDKLRPLIKRAGDHEPIAYLVGRCEFYSLPLVITPDCLIPRPET
                     EHLVEKAILFLRSRPGMQRVLDLCTGSGCIAAAIAKNVQNVQITATDISDAALKTAAA
                     NIEKLKLTDKVQLLCGDLFDPIIEGLDQSRFDLIVSNPPYVSEPEYARLDKNVRDYEP
                     KGALLAGRDGLEVYRRILERVGHFLKTDGALMMEIGYAQGPAVKELLEKTALFKEIRI
                     EKDPACNDRIAIAKI"
     gene            22153..22617
                     /locus_tag="PLE88_04690"
     CDS             22153..22617
                     /locus_tag="PLE88_04690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOM75799.1"
                     /translation="MRVEPRSSDFESTPAIQFPDCTKISEYTITIVPRYCETDQAGVV
                     HHTVYPVWFEMGRTELLRANGLAYSELEKAGIYFVVAQLSVKYRRPCFYDEQLHLTTV
                     CTRITSARVEHTYTLKRPSTGLILAEGSSILACVDKQGKPRRMPEFMYPAES"
     gene            complement(23623..24495)
                     /gene="amrB"
                     /locus_tag="PLE88_04695"
     CDS             complement(23623..24495)
                     /gene="amrB"
                     /locus_tag="PLE88_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOM75800.1"
                     /translation="MNIRKAAVAGQFYGAPAKECREEIEECLPPRDLKIELPESIVAA
                     IVPHAGWVFSGSLAAVAFKAIRQVNGDVDTFVIFGAAHRYYGDRPAVYDSGQWETPLG
                     RIEIDADLSSRIIEQGADADLQAHRSEHSIEVQVPFIQYLFPKAQIVPVVMPITGTEH
                     TFGARIGRLISAINDKKIVCVASTDLTHYGPRYGFCPQGTGPDALRWAREVNDRLFID
                     TAIRLEAEQLVQIAIDKGNACGPAAAAVVVAAAKAMGRSRGLLLGHTTSHDIMLEKFN
                     QPSEESVGYAAIVF"
     gene            24612..25118
                     /gene="purE"
                     /locus_tag="PLE88_04700"
     CDS             24612..25118
                     /gene="purE"
                     /locus_tag="PLE88_04700"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOM75801.1"
                     /translation="MDKTRQSPVAVVMGSDSDAEVMEGCVKQLREFGIDPVVRILSAH
                     RTPAAAGEFAENAESNGIRVIIAAAGMAAHLAGAMAAHSLLPVIGVPLQAKEGPCGLD
                     ALLSTVQMPPGIPVATMAIGKAGAKNAAVFAVEILALNNPELAAKLLQFRKEQAQKVI
                     EKDAKLDI"
     gene            complement(25191..26645)
                     /locus_tag="PLE88_04705"
     CDS             complement(25191..26645)
                     /locus_tag="PLE88_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOM75802.1"
                     /translation="MDTIHGLKKIFAAVLLLVNGQIALLTADELAVVSNKPVLLSPVQ
                     QRMQQEISIDFRDTPIDDVLMMMAKQADVDILKSPKVTGTVTATLTDVPLSEALAGIL
                     ETHGYTYVATDNIIRVVAKEDVQEIREKLVSRVFRVTYADVKEVEQALQRFISEQGSI
                     SANPGTSNIIVTDVESKIQAIQSFIEEIDRVTPQILVEAKIYDVSSKDRFDLGIQWQA
                     GTATSYGEPGTGQRLGNDIGTIGNMYNGVRDSALTGAFNGTISKATDTDGLLRFGILN
                     DNVHIDALLRAEKEDVRAKLLANPRVMVLDNQLAQIKIVEEIPYQELTETSAGGSIGT
                     TSFRDVGIELRVTPHLTRDGLIRLQLNPKFSVRTGDVAVAGVNNTNPQPIVASRETIT
                     TALIKDGQTVVIGGLKKQDVSQQINKVPILGDLPLLGGLFKFQGESTVNSELVVFITP
                     HIVTQPTLSAEEQKHLENTVFVSPRLPETRYGNN"
     gene            complement(26693..27469)
                     /locus_tag="PLE88_04710"
     CDS             complement(26693..27469)
                     /locus_tag="PLE88_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75803.1"
                     /translation="MRKSGLHKQISSIFDGVPMPQNSLSAAASPPSQVPPTAAASHSL
                     EASAASRPTLAQRLSADPSVCISTPAVSSKRPMPLPKSAVPSAPKGSGGFSSQVKKAV
                     FGSAKGQVDSRQKKMAVLVGILSVVFAAVLYVTLGGLGKGKAIAAVSSPDPEQSVPAL
                     QKTIEDWKRPDPVPNDLRDATVPVVRQAVPVQTADTDAEVGDLTVRGILFSQHKPSAI
                     VNGQVVTEGQTINGVTVVRISRDTVEFEANGKRWTRSVQR"
     gene            complement(27426..28220)
                     /locus_tag="PLE88_04715"
     CDS             complement(27426..28220)
                     /locus_tag="PLE88_04715"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOM75804.1"
                     /translation="MRTIAITNQKGGCGKTTTAVNLAAALAVRGQRTLLVDLDPQGHA
                     TLGLGLIPEHCQHTIFDVIVQDDLPVSRILMPSGIPLLMIAPSNVLLSGAEAALMTMN
                     QREYVLYNRLLQADLFFDYCIIDCSPSLSLLTLNALNAASDVIIPVQTHYYSIEGLKQ
                     VLETLSVVQERFNPQLQVRGILLTLAEKNAILSRDVERQMRDYFGQLIFQTVIHRNIR
                     LAEAPAAGQSIITYDPANPGAKEYRQLAEEITYEKVRTTQADFVHI"
     gene            complement(28238..28870)
                     /locus_tag="PLE88_04720"
     CDS             complement(28238..28870)
                     /locus_tag="PLE88_04720"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HOM75805.1"
                     /translation="MKLKKIKLSRYARMLIAVWSLCIVLLVLGYWQWYLPLQAERIEV
                     QRQFSESSQQLEQARLAAREETKQQLKQRLEQSSRTIADFSVPADGVTNLVFQIGQIS
                     NDLRLIDFSSKNPKTSYASTLGSTKRLSEGWLSVEFFASFEQFVRFINQLERNCPAVF
                     VEKLQFRRATAESKGHDIKLELSFLASAQPSAEPVASAAGLTAADKTLKN"
     gene            complement(28873..29517)
                     /locus_tag="PLE88_04725"
     CDS             complement(28873..29517)
                     /locus_tag="PLE88_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75806.1"
                     /translation="MMFSIDLLKGKGLPEKTDLKRIGLKAVPLLIPILAVGGLAASWQ
                     HSKALLESQKAALQTHQEQMALYAEDAAAYSKTLAQINSLQGCLKDISAALAYRIQVS
                     DLLVELVQTLPDSIFLYEMKLDRNSVVEKIQQADSADAKQRLVVRRKLNLILCGFDAQ
                     RSDPAVQEYLDRLKQSARLRSVFEQIKPTARQQGQVDNRNAVYYEIECVLKEQG"
     gene            complement(29514..30590)
                     /gene="pilM"
                     /locus_tag="PLE88_04730"
     CDS             complement(29514..30590)
                     /gene="pilM"
                     /locus_tag="PLE88_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOM75807.1"
                     /translation="MALNLLTHRQIEPAGLDIGTSAVKLVRLRKTSDGYALAAAVRQP
                     IDGGTGDEKQRRLSCAEAVKACFQKAGLKSRSAVTGVSGPEVMIRGFKLGPLPMEAVG
                     QAVAIEAQQVCPLDMKHSVLDYQLIESQPIVSPDGRTVQPKHGVMAVAAEHVLQEKMQ
                     ILAEAGVRVLMIDADALALLNCLNELQLLETGGTAAVVDMGMMFTNVVIYGQDGLPFV
                     RDIHIGGQRIAEQLSQMLAVSESQACRFLAGEPSPEVPQNKVLLALNDAIRPLANAIN
                     ETLRFYSFQEKGAGLDSIYLSGGLAQTGSFVEFLADALPAPVHILNPFEAMPVDDAIP
                     NAAELIQSGPAFAVAAGLAMRMVE"
     gene            complement(30603..31850)
                     /locus_tag="PLE88_04735"
     CDS             complement(30603..31850)
                     /locus_tag="PLE88_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75808.1"
                     /translation="MKTVRQNILPQKEQTGSVLVLVLVIVMVCFIIGTSVLLLGTQSR
                     MASLDQIQDMEARLAADAGVERAVQEINNAVIAKTWSADLLPSASGVEMPFSNAIYSV
                     TTTYDSADGYRIASTGIDRNRSRTVRAALRLKGLFDNAIQCRNNIILKSGTVVQSVDS
                     NVSLNPDDCSQKVIIGTNSTEACSIILNNNVVVDGDVVVGFGGDVARVIKDQGAIFDR
                     SYALSSTLDFPQVSPPPLLGPNTKIEVKTGEKTIGPGGDFPAGGRFSSVKLGQGATLR
                     VIGDCTLYITGTVDMGQNSEILLDPAYNASLTVYLDGNWISDNNSGINNAIQIPSRFK
                     LYGTGPIGQKIDLKAKTDAFGVIYAPNADLTVYSGGNIYGSFVANNFELKSPANFYYD
                     AALKTVSVTDEGARFVISRWNEE"
     gene            complement(31915..>32432)
                     /locus_tag="PLE88_04740"
     CDS             complement(31915..>32432)
                     /locus_tag="PLE88_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75809.1"
                     /translation="MKSMPMKAGFSLTELTLAIAISSIILLAVAVVMLDTQRGWLDSY
                     AKVNSRAIADAAVAKTAFDKVVRKASRTLYYFDAIDDITVFYYSDWLASPQLDRFARF
                     YRNSADPSCLYVQYGDVGTGQVLSEVCLASTVADLEFRPLNGAIEMKLVLQDDHESTV
                     MVTTAILHNDG"
BASE COUNT         8085 a         8361 c         8064 g         7912 t
ORIGIN      
        1 atctgtccga tgccgccgat gggggcagac tccctatgag ggaattatat gtgaaaataa
       61 aactccaaaa tctgaactta tacacccatt tttctcaatg atgcacacgc ccggggcaaa
      121 cctgttctca tcggcgaggc cagccctgtc ttttggcata cagatcaagg cgaagccagc
      181 tggaatggcg atttcgtgcc ttattttaat gttaatagtt gaagggattg aggaaatttg
      241 gcaagatctt tgaaagttaa aaaagaggtg cctgtgaatc tgaggcacct cttagcaaat
      301 aatcactacg cagtgtttat tcttcatcat ccacttcatg ctctatttca ttgtcttcgt
      361 tattgggggg cattgtttgt tcagcaggtt gatatatagc atttaccaac aattctctgc
      421 atttctcaaa ttcttctttt gtttccttgg agccatgtcc tacatgtgct tgactcccat
      481 atttttcttc ccatacataa actaaggcat gtaacatttc atgtaggaaa gtgtcattac
      541 cataagcctt atcaacatct gttattgttt ggcttttttt tctgcgatac aaatatcgat
      601 gctttttatt ttcttattag aagttacttt tgccttactt ttatcacctc catatgatgc
      661 cttgccaatc agtcttggat ctaaatgata catcaaaaat atatgtcgag caacatcagt
      721 gattgctttt tcactagtat ctttcttaga tttatactga gcaattaaat tcctgactac
      781 acctgcttcc cttttattgt aattttgtaa cagattctca tgagtaggtg gataaaagtt
      841 tatttcatca gagcaatgta tattatctga catttcccat ttaaacggtt aaaaacatga
      901 ttttctccta atgacattaa caattttata aaattcgcta ccgcaggggc aagaaaaatc
      961 cattccatgc ccggttttcc tcaaaatccc gtaaatatac ggttttcatg cactcgaaca
     1021 cttacagaca tactttttga cagaccttga ttgctttgac gttcgtaaaa tcaggttttt
     1081 aagcattctt cggcatcatt tatccggtca acaccggctt caagtgtttg attctttcaa
     1141 ggatttcata gaacagcgat cttgaaacca gcacttcagc catttgaaac gtcacatacc
     1201 gagcatgact gacgaccttg gttccgatct taaccagttt ttcccgcatg gtatgcaatg
     1261 accaatcgtt tatggacttg ggcagtgcca accgcctcaa gaagttgcca atattatatg
     1321 ccatacagaa caactggagt cgaacctgat tatcatcgaa atcatggcag gacagccgtg
     1381 tccatttcaa ggcatacttg ccttccctga tccactgctc tgcggttcca cgcttgttat
     1441 aaaacttcac cacattgctg tgtttccagc gaagattggt cacgataaaa ccaacgctcg
     1501 gaaataactg ctctgcgtgc cattccactt tcgcaacgac tcgtctgtct attttccaac
     1561 tggcggcttg gtatcgaaaa ctgtgatact gaacaatcgg ctctttcgga ggacgtccca
     1621 caggacgagt caaaagatgc tcaatgtggc tctggagtat gttattgccc tttaatcgaa
     1681 ttgcatagaa atacccctcc gtttcaagca ggcggtaaat atcaggattg gcaaacgccg
     1741 cagcaccacg aaaataacgg gtaatatagg ttctcagaga gaatgcgacc cacaaaataa
     1801 tccacataaa aaataaaaga tttctgcaag attagtttgc atacccacat tccggtgtcc
     1861 tcatattttt tatccttggt tatcgtgtct tgattcatac gactttccta cagaaaacaa
     1921 gtgttgactt ttgaggtatt ccgccgtagg atgtttttat aagtattgat aaaaacacaa
     1981 tttgggaaaa gacgatgttc acgggtatta tagaagcagt tggcgaaatt cggcaggttc
     2041 tgccgcaggg acagcagatg cagcttgtta tcggtctggg gcgggccggc gagggggtgc
     2101 gggcaggcga cagtatcgca gtcaacgggg tgtgcctgac cgccgccaag gtggagggag
     2161 ggtgtgcggt atgcgatgtg agcgaagaaa cccttaaacg gagtaccctc agccgcctga
     2221 aggtcggctc caaggtcaac ttggagcggg cgatgagtgc ccaaggacgc ttcggggggc
     2281 attttgtaca gggtcatgtg gacggaacag gacgcatcgc agagattcgc agacagggcg
     2341 agtttgcagt gtttcggttt gaagtatcaa cggatatttt aaaccaaatt gttctgaaag
     2401 gatctgttgc catcgacggc atcagcttaa cggtcgcgcg ggtcgatgcg gcaggctttg
     2461 aggtcgccct gattccgaca actattaaag agacctgctg gatgacagca aaggtcggcg
     2521 atgcagtcaa tattgaaacg gacatactgg tcaaaattat ccagaaacaa ttgtcggcta
     2581 tcctgccgca gcagacaatc actgtcgaaa agttgaaaga ttacggcttt tgaatgggac
     2641 tatcaacaac ctgctgggca ataacacggt tattggaatc acgatgggtg atcccggggg
     2701 cattgggccg gaaatcgtag tgaaggccct tgccgacccg atgattcgcc gcttggccaa
     2761 atttgtgatt ttcggcttag acgaacagct cgagtatgcc gcagatttgg cggaggtaga
     2821 accgttctgg ctgcgtcatc cgcatgaaaa aatcagtcgg aactatccgc ggcaggtcgt
     2881 tgtggccgat tacgatgagt attcggttcc cgcttgggtg catgcttcca cacgtgtttc
     2941 aggtgaggca tcgatgcggt tttgcctgga tgccatcgat gcggcccggg acggcctgat
     3001 agacgccatt gttacagctc ccatcagcaa aaccagctgg catctggctg aaagcccatg
     3061 gaccggccat actgagatgc tggccgaccg ctgcaaatcg ccgcgaaagg tgatgatgtt
     3121 tgttgcaggg cctctgaaac tggcattagc cacgatacac gagccgctgt ttgaaatacg
     3181 gcacaaattc acgattgggt gtgtatttga gccgatagac cttctggata tggcactgaa
     3241 gcagtatttc gggatcgcca agccccatat tgcggtggcg ggtctgaatc cgcacgccgg
     3301 cgaggatggt cagttcggcg atgaggagcg gcggattatc agtccggcaa tcctgctggc
     3361 acaggaggcc ggcatcaact gcacgggtcc ctacccggcc gacacgctgt tttaccatgc
     3421 cgttcacggt gcctacgacg gcgttgtggc aatgtaccat gatcaggggc tgattcccat
     3481 caagatggca gcgtttgatc aggcggtcaa tgtaacactg ggactgccga tcatccgcac
     3541 ttcgccggga cacggtacag cctttgatat tgccggcaaa gggattgcca atcccaatgc
     3601 tatgaaggaa gccattcggg tggctgttca aatggcaaag acgagccaaa aaaacaaaat
     3661 ccagaccggt aagtaacagg gcaaaacagg catagtttcc gaaataacag agttcttttc
     3721 aaacagcata acaatgcaga caatatgttt tgattttcct tgttatttgc ctgtttgggc
     3781 tgtattttgg atttcgaagt taaagatttt ccggacttgc tgaatcatgc agaccaaaca
     3841 ggacattgag cggctgctgg cgggagcggg cgtagcgccc aaccggcggc tggggcaaaa
     3901 ctttctgatt gacctcaacc tgatgcgtct gctgctggat gcagctcaga tgcatcagca
     3961 ggatgtcgtg ctggaagtcg gcaccggcac cggctccttg acgcaggcca tcgccccccg
     4021 ctgcgggcag gtcattaccg tggaatacga tagcggtctg gctaagattg cccgcagtca
     4081 gctggcggct gtgcccaatg ttaccttgtt caatatggat gtgctggaaa acaaaaatgc
     4141 cattcaccat gagatactcc aagcacttca gacggctcag gacagcttgg gcggccgtct
     4201 tctgctggtg gccaacctgc cctacagcgt cgcttcctgc gtgatggcca atctcatctg
     4261 cgggccggtt acggcacagg gaatgtatgt aacagttcag aaggaagtag cggaacggat
     4321 ggcggcaggg cccaatcatg aagaatacgg cgtcttgagc attctgatgg ctgccgtcgg
     4381 cagcgtgcat atcctgcgaa agctgcctcc ttcggttttc tggccgcggc cgcaggtcga
     4441 ttcggccatg gtcagctttg ttcgggatga aaaaaaggcc gcccagattc atgatatgca
     4501 tacctttcag gaagtcatcg gcctgtttat gactcaccgg cgcaaaatgc tcaaggcctg
     4561 tgtccggttc gcccctgctt ctttggctca tattcgccac tggccggata ttttcaagga
     4621 ggcctttgtg gacccgcact cccggccgga agaactttct gccgctcaat atatctctgt
     4681 tgccaacctt tgctacgagc aaaggcggta gcatgcaata tgcggcagct tcttttaaca
     4741 gctgaaagga aacctatgca gacaagaaga cagacaaagt ggaaagctgc aatctgtttc
     4801 gggtgggtgc tggttttggc agcgggactt tcggcacaga accggacatt tctgaccggc
     4861 ggggatattt cgatgctcag ccggttggag gagaccggcg tggtttttaa ggacgatggg
     4921 cagccgaagg atttgattaa gctgatgggc gactacggtg caaactgctt tcggctgcgg
     4981 ctgtttgtcg agccgtccgg ccggggcggc gtgattcaag atgtccctta taccatcaat
     5041 ctggccaaac gcatcaaggc ggcgggggct gtttttctgc tggattttca ttattccgac
     5101 acgtgggctg atcccagcca tcagagcaaa cccaaggcgt gggagaatct gagttttgca
     5161 gatttaaccc ggcaggtgca gctctacagt gctgatgtca taaggcaatt gacccaagcc
     5221 ggcgtgcagc cggatatggt gcagatcggc aatgagattg cgccgggatt tctgtggccg
     5281 gacggcaaat tgagcggcaa agagcccgaa gctgaatggc agcggtttac tacgctgctg
     5341 aaggcgggaa tcgccggcgt taagcaggcc gacccgaatg agacaatcca gattgtcatt
     5401 catatcgaat gcggcgggga cgcaaaaaaa accgactggt ttttcagcca tttagaccaa
     5461 tacggagtcg cttacgacat catcggcctc agttactacc cgtggtggca cggaacattg
     5521 gcggatttgc gaacgaacct gacgcagacc gccgagcgat ttaaaaaacc ggtctttgtc
     5581 gtcgagaccg cctatttcaa ccagccgttt gacccgggcc ggggaacctt taaggacaat
     5641 ctggcatggg aaaaatcgcc ggccggtcag caggcgtttt tatgcgatgt agtacagagt
     5701 gttcgggatg tgccggacgg catgggaatg ggggttcttt ggtggtaccc cgaatcggtg
     5761 ccgattcgac aatccggcgg ctggaacaac ggtgcaacag ctctctttga cagcaacgga
     5821 aggcccctgc cggcattgag ctgctttcag ccgccagcag gaaaataaac aaaaatcaaa
     5881 tcgtaatgct tttgtggcgg tggctgtggc attcgatgtt gacagccgcc ggcagcgaag
     5941 cgatatggca cggggcggtt tcaatacaga cggccaatgc ggtggtatcg ccgcccagtc
     6001 ccattggacc cagaccggag gcattgaccg ctgccagcag gtcctgctcc atttgtgcat
     6061 agaatgggtc ggggtttgcc tctccgattt ttcgaagcag cgattttttg gccagcaggc
     6121 aagacaattc aaaattgccg cccagcccca cacccaccac aaagggcgga caggcgctgg
     6181 cgccggcctg cttgactgct tggacaatcc agtctattac ggcttccttc gagtcggttg
     6241 gattaaacat ccgcaagcag cttttgtttt cacagccgcc gcctttggcc atcagggaaa
     6301 tgtgaaccgt ctgtccgggc acgaccgaat aatgaatgac aggcggggta ttggtttggg
     6361 tgttctgccg ccggttgagc ggttcagcca caacgctctt gcggagaaat cctttttcgt
     6421 agccggccgc tacgccttgg cagattgcat caacaattgt cgctgccggc ttgtcgtccg
     6481 gcggcacaat ctgcacatcg gccccctgct ggacaaaaac caccgccaaa cccgtatcct
     6541 gacagagcgg aatcctctcc tcggcggcga ttcgggcatt ttcaatcaat tgttcgagaa
     6601 tctcccttgc ccgaggattg gtttctctgc cggcggcctg ccgcagggct tgtgcaacat
     6661 ccggcggcag ctgaaaagca cagtctatgc acaatgtttc gacagattgg acaacctgat
     6721 tgtaggcaat tgttcgcatc agggaatcgc agcctcatag gttttataga gtgtatttcc
     6781 caagtcatcc gagaccgaga ccgccacgat gtgctggccg gatttgagat catcaatccg
     6841 cattgtaaac cgttcggtca gggtatcgca tacgccgtca tccggcagcc cagctgtcca
     6901 cttttcgttg ctgtcaacgg tgtactgcac cctgcccaat acagacaagg catcggtcac
     6961 tgtcatctgc agcacaatgg aggttccttc gatgcgcagc tgggcatctg taatttgcgg
     7021 agccgtattg tcaatcacca ccggttcgct gatgcgcgag ccggtcagag ctgcttccgg
     7081 cgtattgctt ttgcggtcgc tggccgtaac gcggatttca tagcgtccgt cctcaacggt
     7141 tcgaccgtcc cactcaaaac ggttttgatc cagctggtcc ttcatcggaa tccagaccgt
     7201 ccaccccagc ttgcggaact ccagcgtata ttcgagctga tcccggttat catcgcgggc
     7261 tgaaaaggaa atgtcgatga tgccgacttt tttagggtcg cgtgagggtt cagccttgac
     7321 cgccgcaaca gtcggagcca gattgggaat taccgaggcg gcggttacct gccgcaccac
     7381 cggtgaagca ttgtcgctgc cggccgtcag ggtcagccgg tactggcaga accgtgccac
     7441 aggacaggcc aattcgaccg gaccatcaat aaccgtttcc ggactccatg gtgaaaatgt
     7501 cgcatcgttg gggtcctgaa cattgccgct gcggcaggcc attgtaatcc ggccgccttt
     7561 ggggatatcg gcttcaatct gcagttttcc ccagcgtgcg ggctgtccgg catccagcag
     7621 agctgactca aatgtgccgg tcggcgccac agcactatcc agctgaatca gccgagcagg
     7681 attggacagc cccagataga taaccccatt gaccggaagc gctgaagtaa tctgagttga
     7741 tattttatcc tcaaacacca gtgcctgttc atcggctgcc ggatcaatgg caaacagttg
     7801 tcccttattg ccggtaccga cccacagttt gccatcggcg caatggagcg aatacagaac
     7861 agccatttca gtgaacatat cggtcacaaa tccttccggc gttattttgt agatatgacc
     7921 ggcggccttg gctgccggcg gtgccggcgg ctggggagcc ggcgcggctt ccttcttttc
     7981 ctcctttgtt tcgctgctgt tgggggtatt caatgaacct tccagcggaa caggcctttt
     8041 gtcattgtca acatcgggcc ggccgggggc acggcgctgg ggggctgcgg gggtctggag
     8101 ctggagcatt gcggcctgtg cactggttgc agcggcatag acattgccgt cggaatcaat
     8161 ccataatgcc gggatttcgt cctgctcggc atcatacaac acagaggtct gtttttgaac
     8221 ggtatcgatt ttataaatca gaccgcgctg gtcggcgccg gcgtagagaa tatgatcttt
     8281 tatcgccagt gaaaggatat ttttatcttt ggcatcccag attacttcgg gattctggca
     8341 aaatgggtct aatcgaaaga taagcccctc ggggccggta gccagataga tattgttatc
     8401 ggcatctgct gcaattgcaa aaatatactg cacacgcgga tcggtaaaca ccgtttcggc
     8461 ttggccggac agacgaacca gccgcccctt tgtcccgctg acgccaatca acaaacgccc
     8521 tgccacatcg cgcgccagag caaagatatg ttcgttgcgg atagtcgaat cagctgattg
     8581 gttgggatcg gcagaatcac gcggatatac ctgttcggct ttccggccgt cataacggat
     8641 gactgccgca ttgggaccgg ttcccaaaaa cagcgttcca tctggctcgg ccagaatagt
     8701 atgaatcgac catacatcat tcagcaggcc gtcgcagtgt atttctctgc tttgccgcgc
     8761 cagccgcagt gtgccggcgg agtctgcaat gaccttatcc gttttgcctt tgccgaaatc
     8821 cgccgccgtt tgatggcgga tgatggcaga ggtaactgcc catccggctg cccctgctgt
     8881 cagaaacagt cccaaaaccg cagctatttt cagtttcata agtctccttt atatgcactc
     8941 tgcttacttt tctactgtaa tttcaatctc ggcagttcca tcgaccaccg catccaaaga
     9001 ggtgctgcct tccgcccaag cattgtaggg ctccagcggc tgaaggcgtt tggcatcctg
     9061 catcagcagc actttcgtgg gaggaagctg ccccagctca tgctggcgga taaccagtcc
     9121 tgttgctgga atcggcatca cagcatacag ccggtcacgg cggtatttga acaacccttc
     9181 gaggccggtc ttgagggtgg gcaggtcgta ggcacggaat ttataagggg ctgccttgga
     9241 gacaaaggcc tgatagccgg cagcgccgaa aatgtgtatt ttatactttc cggcatcaag
     9301 ggtttccggc accttcaact ctatggctgc tgttttttcg ggggccctaa atgaccggag
     9361 agttacttcc gctgtgatgg tttgaccggg acggacgcgg gcattagaga cgttcacttt
     9421 ccagatggag gcggctatgc tgacgggatc aatttcgatt cgggcattga tcgagcgaat
     9481 cggaactggg ccaaaaggat tattcatcag caagtctaca gcggaggcag catcagaaac
     9541 aacatcgctg agctggcgcc cggaggaaac attatcaatc agaagcggtt gtccgtcttc
     9601 cagaacaatc tgaccggcat agcggactgt atgttcggaa ggcaaaggcc cctgcatctg
     9661 agcggcaccg ctgagaacaa cctgcagaat ggacggcgtc ataccgcggt cgtcggccaa
     9721 aaagcagtta tatacccgct gccgggggtc attaaaacgg ctgacttcga tttgcagggg
     9781 aatggtgcgg ggtattttgc cgattgttcc cctgacggcg cagttttggt caaattccag
     9841 agttcctaaa atgggtcccg gtgaggacaa tttgaaagaa tatgagcggc tggccacaac
     9901 cgtatgcacc actccggcgg caatgggcag attgactgga cctaaaccgt taagactatg
     9961 cccgaaacca tagatttggt caccgacgat tgccgttgca gtgccaatgc cggacagcga
    10021 tatatccccg ccgcatagga cgactgctag cacgctgccc ggttcaaaat tgcctgcatc
    10081 ctccgccgtt gccgttaaaa cggtaggtcc ggcggctgcg ggcatcagcc cgatccggcg
    10141 caatacagtg ctgtactgct gacatacttc agccggcaag gaggttgaca gcggcagcag
    10201 aaggtcgtta tcggtccggc gttcctgcag acgctccata tattgacgat aatatacctc
    10261 taaatcaagg ggttgtgaaa aatcgaaggc tgctgtagtt gaagcagaaa cctttgttcc
    10321 ttgggcagta ccaacctcaa gcatatcttt aataggacga acaagataca gagaatccag
    10381 agacccgtcc cagcccgcag ccaaagcacc cgccaaacgc ccatcaatat aaaccggcga
    10441 gccgctgcag ccatgcacag caccggcttg gttgaagcgt tcatcggtgc ccaaaacaag
    10501 aatcatatcc tgccccgggc gctgatttcg cacaacactg aggactttga gggggaattt
    10561 ttccactttt gaaccggaaa aaacagtcaa gcaataggca tccatatcag gacgtacttc
    10621 gtcgagactg atgcaatcgg cagggggtaa attctcagca cgccccatct gccctattag
    10681 aaccatccat agaatgcaga atatcgcttt tccggttcca ctgtatctca ttagaattcc
    10741 ccgtgcctat tcgttcttta tcgattttat agaggaaaaa ttagtatcgt caaaaactgt
    10801 tgcatgtcaa aaacaatttt cctataatgt ttcggttgtt ttaaggccgg ctgattttgt
    10861 aaaggttctt tcaggcagta aagccggagc aataacaaaa taattataga ctattttaat
    10921 cgaaaggtca ctatggatgc cggacatcgt catgagctga aaaccaatga attggcccag
    10981 cagcttggtc agttgcccaa actcgccaaa gaaaacgcca actttattat cgggacggca
    11041 ttgattgtca ttggtctgat aacctggccg gtgttcagcc ggatgcgtct tcagaaagat
    11101 atggctgccc aaagcagcat cacccagtcc attcagatgc tggacaaaga tgtctatgat
    11161 attctgcagg cacaggacgg agatgcccag aatcaggcgc tcggaacgct tttagtgaac
    11221 gcggataccc ttctggaaaa agcagagaaa attgacaatc ctaatctggc cgctttagcc
    11281 cgtattaaag ccgcacaagc cattcgcacc gaactgcatc tgcgcaaaga cgtcagcccc
    11341 gaaatgctcg aaaaccagat ccagaaagca aagagtgcct atgagcaggc tctcgaatca
    11401 gccaaaaccc caacgcttaa ggcaatggcc aaattggggc tgggattatg tgctgaagag
    11461 ctcggtcaaa ccgctcaagc aggtgatatt tacaaagaaa tcgtcagcga taaaacattt
    11521 gaggcgacag tttttcccaa gcaggcccag cagcgtctgg acagcttaga agataacgcc
    11581 cagacattta catttgctcc tgcgcctgcg gaagcacccg cccctgcagc acccgtccag
    11641 acaagccaaa ccgagccgtt agccgttatt gaggccgcac cagagccggc agttccggca
    11701 acacccgaac agacatcgag ccaaccttaa tccatgtctg atttgaatga tatcccggcc
    11761 tctcctgttg atgattccga tgatgaggcg ctggacaatc aagttattga gctggacaga
    11821 aatctgaccg atgaagaaac cacacagctg gccgggcggc cgctgcggtt tcgggtaggg
    11881 tccaatctga agtttaaccg gcttgataaa tacctgtgcg gacgattcag ccaattcagc
    11941 cgcacacggc ttcaagccct catccgggag cagggcgtca atgtcaacgg ccggccggcc
    12001 aagcccagcc ataagcttca ggccggcgac atcattgagc tgattctgcc ggcacgcgag
    12061 ctgcgtgagc tgactgccga aaatattccg cttcatatct tgtatgagga tgatgatatt
    12121 ctggtgctca ataagcaggc caatctgatt gtgcatccgg cgcgcggcta taaaagcggc
    12181 actctggtta acggattggt ttaccatttt cagcacctgt catcgtgtcc ggatgacatc
    12241 cgtcccggta ttgttcaccg gctggaccgc aataccacag gctgtctggt tgttgccaaa
    12301 aacgacacag cccactggaa gctttcccgc caatttgccg accgcacaac gaaaaaaacc
    12361 tatcttgcta tcgtccacgg cacaccggaa ctgcattctg actgcatcaa gcaaccgctg
    12421 ggcatgcatc cggtcatccg ggaaaaattt tctattcggc ccgatatcgg caaagaggcg
    12481 gttacttttt atgaagtgct ggaagcattt cgcggctttt cattgctcaa actcgatctt
    12541 aaaaccggcc gtacgcacca gattcgggtc catttatcgt acatcaaaca cccgattgtc
    12601 gccgacgaca tgtacggcgg caaactggtt tacccatggc agattgaaaa ccgccagccc
    12661 gccgccgaag aaccgctgct ggcccgctgt gccctgcacg cctggaaact ggagctgact
    12721 catccggtca cccagcagcg aaagactttt gaagcacccc tgcctgatga tatgcagaga
    12781 tttttggacg ccctgcggac ataccgctcc ttgccagcca aagcctaaga ccccttatac
    12841 cgccagtgcc aaggttcgaa ggccattgca gaaccttccg gataagacat ctcaaagccg
    12901 aatcggcctg cattttcgag catccattga tattgttttg ttgctgtcat ttcttcggga
    12961 ttatcctgcc cgttgatacc ctgttctgta atgatgtcta ttgcctgatg ttgtggccag
    13021 ccatgttcac tgtaccccgg ccaggcagca aaacgggctg ttttcagaac tgaatactcg
    13081 tgatttttga gatacacgag aaacagatat aactgatatg ccgccggccg atagcccgat
    13141 tccacataaa tgcctgcccc gatatcttgt ttcatctgac tgaccattgc cgaaaaggcc
    13201 tcatagaccg gtatcggcaa aaactggacc ggcaattctt ctgttcggcc gtcggttcgt
    13261 gttattgtct ggccgcgaag agcaatgaaa tcgggcgtct gggtcggtat cccctgatag
    13321 ggaatggtca attggagccg ccggacatcc agattcaaaa atctccgcag aaaactcttt
    13381 tgttttttgt ccagcgggac aaacaaatcc tcaaacgtta gtgccgctaa atcttcctga
    13441 atccgccgct gccgtatcat cggctccagc cggtctataa tactttcgac gtgctggcga
    13501 tcctcgggcg aaagtgcggc aatattgatt cggccggctg tgcatccgta taaaaatccc
    13561 atacagacag ctgctatccc gattgaaaat cgcaaaaaaa cgaatttttt gatttgttct
    13621 ttacgcatct gcctgcccag aagtataagc agatagcgga ggcaagtaaa taggcctatt
    13681 gtgcccgtat tgctgaaaca attcaagttt ccgttgttta tcttgagaaa ccagacgttg
    13741 gctgtataat cgctggttat ggtaaaagaa gcggacaatc tgacgccggc aatgaaacag
    13801 ttccggcatt tcaaatcgaa atatccggat gcgatactgt tttttcgaat gggcgacttt
    13861 tacgagacct tttttgagga tgcccacatc gtatcgcgtg ttctggggct ggtgctgacc
    13921 agccgcggca agagcgggga tacgcctatt ccgctggcag gactgcccta tcacgcagtt
    13981 gacggctacc ttaaaaagat gcttcaggcg ggctacaaag tagccgtctg cgagcaggtt
    14041 gaggatccta agctggccaa gggtgttgtc aagcgggatg taatacggat tgtaacaccc
    14101 ggaacgctga cggatgagat gctgctggaa gccaaagagg acaactattt ggccgctgta
    14161 tggctgggaa cgaaacaagc ggctctgtca tgggtggata tctccacagg tcatttcttt
    14221 attcgttcgc ttgcggacaa acacattctg gatgagctgc tccggctggg ggttcgggaa
    14281 gttctgctgg ccgaatgccg aggccagctg tttgaagcgg agcaaaaaaa attagtccac
    14341 cagattcggc agcttaccgg agctgtcata acagaacggc ccggatggta ttttgacccg
    14401 tttggggcaa aagaaaatct gctcaagcat ttcggagtca gcacgctgga aggtttcggc
    14461 atcagcgagg attttgaagg catctgtgct gccggggcta tccttgagta tctggatgaa
    14521 acacaaaaaa ccgcccttaa ccatattacg tcgcttcgtc tggtcaaacc cagtgactac
    14581 ctgcagattg atcctgtttc actgcggtca ctggaagtgg tgcgaacgat acggggggaa
    14641 tcatccaagg gaacgctgct ggactgcata gaccagactg ttaccggcat gggatcgcgg
    14701 atgcttcggt catgggtatg catgccgctg cgctgcactg ccgaaataac agcccggcag
    14761 gatgccattg aagaactggt taacaacgat gcgttgtgca cccagatacg caagcnnnnn
    14821 nnnnnatcgc cgatctggaa cgcatcgccg cccgcatcag tacctatcgc acctcgccga
    14881 aggatttggt ggcattgggc atcaccttgc ggaccattcc gcaaattcgc caattgctgt
    14941 cggactgttc ggcctcaatg ctccaaagcc tttgtggccg ctgcgacagt atggacgaac
    15001 tggcggacct gctggaacag gcgattgagc cggaagctcc ctcgcatctg cgagacggcg
    15061 gcgtaatccg ctccggcttc agcgaccagc tggataacct gcggtcactt tccaaagacg
    15121 gccaaagctg gctggccgaa taccagcagc gtcaaattca gcggaccggc attgataagc
    15181 tgaaaatcgg tttcaacaat gtttttgggt attacatcga agtcagccat gcccatgccg
    15241 ataaagttcc ggccgattat gtacgcaaac agacaataaa aaacgctgaa cgctacatca
    15301 cggacgagct gaagcaatac gaagaacaag cccttggcgc ccgggaaaaa gcactggagc
    15361 tggaatgccg tttgtttgag caggttcgtc aggaaacaac ccgttatatt cgacggctgc
    15421 aggaattggc gcacgtgctg gccgtatgcg actgccttgc cgccttggcg tatctggcgc
    15481 ggcagaatca ttatatccgg ccgaaagtta cagacggcag ggaactggtt attattgaag
    15541 gcaagcaccc tgtgctggcg cagatgctgg gagctgaatt tgttcccaac gatataatcc
    15601 tgtccgaaga cggcaaagat gcagccatta tcaccggtcc gaatatggcc ggcaagagca
    15661 cctatatccg ccaaactgcc ctgctggtgc ttttggcgca aaccggctca tttatacccg
    15721 ccaagaaagc ccatatcgga gttgcagacc gtattttcac gcgggtcggc gcctcggacg
    15781 aactgacccg cggtcagagc acctttatgg tggagatgac tgaaacggcc aatattctca
    15841 ataatgccac ggaccgttcg ctggtaatcc ttgacgaagt aggccggggg acgagcacct
    15901 acgacgggct ggctttggct tgggcgatta ccgagcatct ggccaatgtc attaaatgcc
    15961 gtacgctctt tgccacacat tatcatgagc tgactgaact ggggcagctt ttggccaatg
    16021 tgaaaaatct gaatgtagct gtgcgggaat gggccggaga ggttgttttt cttcataaaa
    16081 tcattcccgg aggaactgat aaaagctatg gaattcacgt ggccaaattg gccggcatcc
    16141 cgaaatcgat cctgacccgg agcagcgaaa tcctcaagga actcgaaaat acctttgcca
    16201 aagaagcggc cggagaacat ctttccaaaa acaaaactgc tgatgaacag ctgctgtttg
    16261 tccaaaagca taaatctgta ctggacaagc tggccggact gaatgtcaac aagttgactc
    16321 ccatcgaagc aatcaatctg ctggctgaaa ttcaacagga gataagccgt taaacaaggg
    16381 cttcaggcag gcataaagtc cgataacttt tgtctgtccg cagcaatcat aaatgctctt
    16441 tatttttctg ctgaagattt tttttatttt ccttttccaa tatccgacgt ataaggtaga
    16501 aacaagagca agaagaaaag aggaaacaaa tgtttcaaat gatagataaa ctgcagaaag
    16561 tagtcaattc ccttctgggt ttatgcgaag agggaggagc tggggtttcc gaagaagaca
    16621 ttgtaagccc cgaatatcat aaatatgaac cgctgggaat tatgattctc aaaatgcagg
    16681 atggtgtgct tgaaaaacgc tatttcctgc ggatggaaaa atggctgctt tgtgaccctg
    16741 cagcggttga atattacatt gattttcaaa tgctgacggc ccttttgcat gagcatttca
    16801 ataaaagccg gctggaaaaa attgcggatt ccttcagaga gataataaca ggcaaagccg
    16861 ccgcaaactc ctgacacccc tggcagccca aatggtgctt atttaggcaa attagaacag
    16921 catttcgggg attatagtta tagcctgctg cgggaccgta atgttaatgc agtcgaaaag
    16981 ggagtattta gccgttgctg ggttttgaag ttctgacgtt gtcttgctgt gttgtctttc
    17041 tcggccacag cagaatatca tcttttttca gttttggatg attaatcccg ccgtcatcga
    17101 ccccgttagg accaagcgaa tacaaacgga attcagaatc caaacgctca tatacaaacg
    17161 gcctgctggt aagcggatcg ataagcaatt catcgctgtc cctttcgata tgctcaagac
    17221 gctccggcca ttggccatgc tgcaaaaaat accgcttcaa gcccgaaaac agatgcatct
    17281 gacgaacaat ggcctgatgc cgccggtact gaccatccag tgcccaaaag tagccaacct
    17341 gctgcattgc cagccagtcg attatactct gggcattcag ccccagtgtc caaacatatt
    17401 tgatgctgaa attcggcagc tgctggcctt tctgcacctg cagggaataa tagtcaaagc
    17461 gtttatcgat cagctgggcg agccgctgcg gcgacatcgg cagactcagc cacagtccga
    17521 tgactgccag ccggttgatt tgatgctgat tgagaaacaa tctccgggca ggatagccca
    17581 gcccttcctg caaagcagca gctgcattgt gtgagatgcg aatccggccg gcctcattga
    17641 cttcatacag cagggctgca atatttttcg tcagaagctt ttcccgctgc aggatatgcg
    17701 gccagttgcg cgtccatcgg ggattgaggt cttcaaaaac atcttcaatt ttcaccgccc
    17761 agtcgggatt ttcgcaatac cgcaccaaat aggtttccaa tgccctagcg cccatcagct
    17821 ccacataaaa cgctccggct tggtcaaaaa gggtctgctg ctggtacaaa tgctgcgaca
    17881 gatttacaac cgccagcatt ttttcgagag cctgttcctg atgcccaaga tataaatcgc
    17941 ggttagctgc ccgcagcagc agccgtgccc agcctttgag ccgattgatg cgctccatct
    18001 ggggcttcag cgccgccaga ttatagggaa tttcaaatcg gcactgttcc acgcgggctg
    18061 ccgccgacag cctttcaatt gccggctgca aactcaacat ccaggcatcc agctggggat
    18121 aagcagcagg atcccacggc tggctcagtg ttaatgcatc ttcctgcgga ctgctgaagc
    18181 tgtagtaaaa aatagtctcg ctgtatgtat caaggatggg ctgatacaaa tcagcggcat
    18241 tttccatccc ctccggcaga taatcccgct caatttcagc cagtgccttg tcatactgat
    18301 agacgcgcca tcggctgctg ttatgttccg gcagattgac ccatccccag acaaccagca
    18361 aaaacagagt tagtcccatc catacccacg gctgtatctt tctgggcacg aaaatcccca
    18421 ccgccggaat aactgccaga cagaccagca ccttccacgg cagttcaaaa taaataccgg
    18481 taaaaaacaa aacaccaaag gcaacaatgc tgagccaccg aagcccccac aaaaagcact
    18541 tccagacagt gtaagtgccc ttaaaaaaac gctttacggc attcttatcc aatccagccc
    18601 cctgcctatc tgcggcggcc gctttggtct ttagttacca acttccacgc tgaccgcttg
    18661 acgtggagcc cataactaac aactcaaacc tgccgttcca tacgcacttt gacaacaatt
    18721 ttcttgatcc gctggcccac accggccgaa cagcccggca tatggacttc gttgggccag
    18781 aagacggcaa acattccggc cttcaggacg attcggctca tctgcgggca atgcttataa
    18841 aaagcaacat ccttacggct gtcgtaaggc tgctcttgat caaggccatt taaaaccgca
    18901 taaccgatgc attcggtgcc tgacgcaatg tattgaatat caagatactg ttgatgaatc
    18961 tccggccggc cttgttcaaa cggcttggat tggtactcat caacaatgta aaacagggca
    19021 tcgccctcaa tttcataccg tcccggcagc gactgatgca cagtgtcctt cttcagcagc
    19081 tccagcccag cggcaattcg cctgtccaat ccgttgtata atgatgcatt attgagatga
    19141 tctgctatca tccgttattt tccttgtctg ccggccagaa tagctcacaa attcagctta
    19201 tcgtgcaggc acgctttaac ctgatccaaa gaaacgcgga tctgcaccat gctgtcgcgt
    19261 tcacggatgg ttacagtgtt atcttccttt gtttggccgt caacagtcag acagaacggc
    19321 gtgccagctt catcctgacg gcggtagcgg cggccaacgg cgcccttttc gtcatagaag
    19381 caggcaaaac tgcctttgag gtcatcatac agtttccggg caatctcggg catcccatct
    19441 ttcttaacca gcggaaacac agccgcctta atcggcgcca gatgcggatg aaaccgcagc
    19501 agactgcgcg gctcgccgtt aacctcgtcc tcatcgtagg catccaccag aaacgccaat
    19561 gtgctgcggt cgatgcccgc cgatggttca atcacatacg gaacatatcg ttcacgggtt
    19621 tcttcatcga aataagacag cggccccttg acataatcgg ggtcctcctc cttaagcgtt
    19681 attttcgtca aatcgccctt ttgctcgaac cagtcattca ttgtgcgcat cccgcgctga
    19741 tgctgccgca ggtcgtaatc ggtgcggttg gcgattcctt ccagctcctg ccaatcgcct
    19801 tgtccaaagg ggaatttgta ttcgatatca acacaggcct tggcataatg cgccagttca
    19861 tctttttcat gttcgcggaa gcggagattt tcttttttaa tcccaaggtt cagataccag
    19921 ctaaaacgct gctgcttcca atgatcgtac cattgttcat cggtaccggg cttgcagaaa
    19981 aactccagtt cggcctgctc gaattcgcgc gtgcggaaaa taaatgcttt ggttgtaact
    20041 tcattgcgga aggatttgcc gatttgaccg atgccgaacg gcaccttaac ccttgtagaa
    20101 tccagcacat tgcggaaatt aacaaaaatg ccctgggccg tttcggggcg gagaaacagc
    20161 gtcatctggt catccacatt ggcccccatc tgggtttcaa acatcaactt aaaagccatc
    20221 ggctggctga actgccctac tgctccgcag gccggacaga ccttgcggct taggtcgtgg
    20281 ggcttgcctg cctctatctc gatattaata tgttcggtat gttcctgatc ggagccgccg
    20341 tcgcttttat cctgcagatg gtccaccctg ctgcgggtat tgcacttttt acagacagtt
    20401 acaagatcgg caaatttatc ggcatggccg gacgccttcc agaccatcgg atgcatcaaa
    20461 atcgagcaat ccaagcccac aacatcgtcc cgcatctgca caaccgcttt ccaccatgca
    20521 ttgcgtacat tgcgcttcag ttctgctccc aatgggccgt aatcatagca gcttgcaagg
    20581 ccgccgtaaa tctcactgga ttgaaaaata aatccccgcc gtttgcacag cgatacaata
    20641 tcgtcaagct ttgctaattt ggccatacct ttaccttttc tggagttcag aatacttctt
    20701 ttgttcagaa agtttttatt ttaccggtta tatctaaaat tacaagacga agattttgta
    20761 cataagagat tagattgtgg gcaacatttg ttaatgtaca gcgtctttct ataaaaaagc
    20821 tttatgttaa gcaagactta ggacgaaagg ggaataccaa tggcgcacat agggataccg
    20881 ggcggaatcg agtggctgac catctttttt atcggcttct tcggcattgg catgaaattg
    20941 gcttttatgg tatttgtcat cgtttttctg ataaaaatca acagttctat cgaaagtgta
    21001 aagaaaaaac ttgaccagct tgagcagaaa agatgaacat cgggattaca caagtatccc
    21061 aatcgaaatg aattattctg cgcaggcatc ataggcgggt attaactatt gcaggtatag
    21121 ctattccccg aaaaaattat atcttagcaa ttgcaatacg gtcattgcag gcaggatctt
    21181 tttcgatgcg gatttcttta aataatgcag ttttttcgag cagttctttg acggccggcc
    21241 cttgtgcata gccaatttcc atcatcaatg ccccatccgt cttcaaaaaa tgacctaccc
    21301 gctcgagtat ccgccggtag acctctaagc cgtctctgcc ggccaacagc gcccccttcg
    21361 gttcataatc ccgaacattc ttatccagcc gggcatattc cggttcgctg acatacgggg
    21421 gattggacac aatcaagtca aaccggctct ggtcaagtcc ttcgataatc ggatcaaaca
    21481 aatccccaca caacagctgg actttgtcgg tcaatttcag cttctcgata ttggccgcag
    21541 cggtcttcag ggccgcatca ctgatgtctg tcgccgtaat ctgtacattt tggacatttt
    21601 tggcaatggc agcagcaata cagccggaac ccgtacacaa atccagtacc ctttgcattc
    21661 ccggacggct tcgcaggaac agaatcgctt tttccaccag atgctctgtt tccgggcggg
    21721 gaatcaagca atcaggcgta atcaccagcg gcaacgaata gaattcgcac cgtcccacca
    21781 gataggcaat cggctcatgg tcccctgctc gtttgatcag cggccgcagc ttgtcaagct
    21841 ggggcttttc gaccacacgg tcatacagta tgtaaagctg gatacgctcc aacccaagta
    21901 catagcacag cagcagttcg gcactcagcc gaggggaatc cacacctttt tgctctaaat
    21961 agccgctgac ccattcaagc agttttttta ttgtccatac ttccatagcc attttttaat
    22021 actttaagtg tctttttgct actctttttt tgtcttatcc ttaacatccc ttaactgata
    22081 cttgattgtt cagtttatgt tggttatcat tagcatcaat tacaaaaaac agatgtgatg
    22141 aaggggtaat atatgcgtgt tgaaccgcgg agcagtgatt ttgaaagtac gcctgcaatc
    22201 cagtttcccg attgcacaaa aatcagcgaa tatacgataa ccatcgtgcc gcgctattgc
    22261 gagaccgatc aggcaggcgt tgtgcatcat acggtttatc cggtgtggtt tgaaatgggc
    22321 cgcacggaac tgctgcgggc taacgggctg gcatacagcg aactggaaaa ggcagggatt
    22381 tactttgttg ttgcacagct ttccgtcaaa taccgccgcc cctgcttcta cgatgagcag
    22441 ctgcatttaa ccactgtctg cacccgcata acgagtgcac gcgtagagca tacttacaca
    22501 cttaaacggc cttccaccgg tcttattctg gctgaaggaa gcagtatctt agcttgtgtg
    22561 gataagcagg gaaaaccgcg gcgtatgccg gaatttatgt atccggccga gtcttaaagt
    22621 ctgccagaaa aaatttctta aactcaaatc acggccaact tcaaaacaga ttgttgtacc
    22681 ccgaatgcaa ctatttatat tcaagcaact tacaacttta atgatgcgct cttacccgga
    22741 agtttatgtt ggcaggcaat tgcagctata aaaaaattaa gccggctaat tatttttacc
    22801 ctccaaatgc cccataatat acggaaaaaa tgtgcaataa aacatctttt ctgtgtttta
    22861 taaatatttc cttatatttt ctttgcattc tcttgaaaat tcatttgcaa tctgatatag
    22921 taacgtttgt cgttattgat aagtacggca taaaagcata aatctggggg gaaacagatg
    22981 accgcttttg aacatacatc tgaattaaca ctggcagtcc aaggggcacg cagtccaccg
    23041 gccgaataaa aaggtaacaa ttttactcaa aatgaattta gtgatttcat cgctttcgtc
    23101 ttggtaaagg ccatatcatc taaaatctaa aaaggaactt caacatgaca ctttttacta
    23161 tccaatgcaa cgctttcaaa aatccaccga tgactgcccg tcaaggccgg gggccgccga
    23221 acttgaaaaa aaagatcgtt gactgcagcg agtttccaga ggccttttta ttttgtatga
    23281 tatatataga aacgggaacg ccctgattta cggaaacatt gatatgccgc cgaggtacaa
    23341 agaaactgtt ctgatacatt tttatcggcc gtgctggaat gctcatgaac ggggcccgcc
    23401 gggaagaaga tgggaaggtc ccaaaaacgc tctcataaaa taacaacact gcagccaaac
    23461 aaccgaatac tgcaactcta tatatcccag ctgttattat aagtaaaatt ccctttccct
    23521 ccttcctaaa gaaacaggaa gattaagccg gcagggtagt cctgccggcg gtatattttt
    23581 tctattccgg caacgggtta ttgcggcggc agcggccgcc tctcaaaaga caatcgcagc
    23641 gtagccgacg gattcttcgc tgggctggtt gaatttttcg agcatgatgt cgtgactggt
    23701 ggtatggccg agaagaaggc ctctgctgcg tcccatggct ttggcagcag cgacgacgac
    23761 agccgctgct gccggaccgc aggcattgcc cttatcaatt gcaatttgaa ccaactgttc
    23821 tgcttccagc cggattgccg tatcaataaa taggcgatca ttaacctcgc gagcccatcg
    23881 cagagcatca ggtcctgtac cctgcgggca gaaaccgtag cgggggccat agtgcgtcag
    23941 atcggtcgag gccacacaga caattttttt gtcgttaatg gcgctaatca accgtccgat
    24001 gcgcgctccg aaagtatgct cggtcccagt tatcggcatt acaaccggca caatttgggc
    24061 tttggggaaa agatactgga tgaagggtac ctgtacctca atgctgtgct cggagcgatg
    24121 agcctgcaaa tcagcatcgg ccccctgttc aatgatacgg ctggacaaat cagcatctat
    24181 ctcaatccgt cccaaaggtg tttcccattg gccggagtca tagacggccg gcctgtcgcc
    24241 ataatagcga tgggctgccc caaagataac aaacgtatcc acatcgccgt tgacttgacg
    24301 aatagcctta aatgcaacgg cggccaatga accgctgaaa acccagccgg catggggcac
    24361 aatggcggcc acaatagact cgggcaattc tattttcaga tcacgcgggg gcaggcattc
    24421 ttcgatttcc tcgcggcatt ccttcgctgg tgcgccgtaa aactggccag ctacggctgc
    24481 ttttcgaata ttcatcggaa acctcctgta ttctctgctt gcaaatatcc gcttcagcgg
    24541 atatatttaa ggattgaggc tgcattctgc acaagctaaa ttatattgta actgctaaaa
    24601 gggaaaatac aatggacaaa accagacaat cgccggtagc ggtggtgatg ggttctgaca
    24661 gtgacgcaga ggttatggag ggttgtgtca agcagctgcg ggaatttggg atagatccgg
    24721 ttgtacggat tttatcggcc caccggaccc ctgctgccgc tggcgaattt gcagaaaatg
    24781 cagaaagcaa tgggattagg gtaatcattg ccgcagccgg gatggctgct catctagccg
    24841 gggctatggc agcccattca ctcttgcctg ttatcggtgt gcctttgcag gctaaggaag
    24901 gcccttgcgg actggatgcc ctgctgagca cggttcagat gcctccggga atacccgtgg
    24961 caacgatggc tatcggcaag gcaggggcga aaaatgcggc ggtgtttgcg gttgagattc
    25021 tcgccttgaa caatcctgaa ctggcagcca aactgctcca gttccgtaag gaacaagcac
    25081 aaaaagtcat cgaaaaagac gccaaattgg atatatagca ttaggttttt cgaagccatt
    25141 aaaaaaacag ctggggaata aaatccccag ctgcatacaa caaaatcgga ttagttattc
    25201 ccgtatcgcg tctcaggcag ccgcgggctg acaaagacag tattttcaag atgcttttgc
    25261 tcttcagcag acagggtcgg ctgagtaaca atatgaggtg taataaagac caccaattcg
    25321 ctattaacgg tactttcacc ttgaaactta aacaggccgc ctaaaagagg cagatctccc
    25381 agaatcggaa ccttattgat ctgctgggac acatcttgtt ttttaagacc gcctatcaca
    25441 acagtctggc cgtctttaat cagggcagtg gtaatagttt cgcggcttgc gacaatcggc
    25501 tgaggattgg tgttgttaac tcccgctaca gccacatcgc ccgtccggac gctgaacttg
    25561 ggattgagct gaagtcgaat cagaccatct cgggtcaaat gaggcgtcac tcgaagttcg
    25621 atgcccacat cgcggaagga agtggttccg atgctgccgc cggcactggt ttctgtcaat
    25681 tcctgatagg ggatttcttc cacaatcttg atttgtgcca attgattatc cagcaccatt
    25741 acacgcggat tagccaacag tttagcccgc acatcttctt tttcggcacg aagcagtgca
    25801 tcgatatgaa cattgtcatt taatatccca aaacgaagca gaccgtcggt atcggtggct
    25861 ttgctgatgg tgccgttgaa agcccctgtc agcgccgaat cccgcacgcc gttatacata
    25921 ttgcctatgg taccgatatc attgcccaga cgctgaccgg tgccgggttc gccatagctg
    25981 gtggcggtgc cggcctgcca ctggatacca aggtcaaaac ggtcttttga agagacatcg
    26041 tagattttgg cctcgacgag aatctgcggg gttacacggt caatctcttc aataaaagac
    26101 tggattgcct gaatcttgct ttcgacatcc gtaacgataa tgttgctggt gccgggattg
    26161 gcggagatag aaccctgctc ggaaatgaaa cgctgaaggg cttgttcaac ttccttaaca
    26221 tcagcatagg taacgcggaa gacgcggctg accagttttt cgcgaatttc ctgaacatcc
    26281 tctttggcta caactcggat gatattgtcg gtagccacat acgtatagcc atgcgtttca
    26341 aggatgccgg ccaaggcctc cgacagcgga acatccgtca gggtagcagt tacggtgccg
    26401 gttactttgg gacttttgag gatgtccaca tcggcctgct tggccatcat catcagcaca
    26461 tcatcaatcg gagtatcgcg aaaatcaata ctgatttcct gctgcatccg ctgctggacc
    26521 gggctgagca gaacaggctt attgctgacg acggcaagtt catcggccgt cagcaaagca
    26581 atctgcccat tgacaagcag cagcactgcg gcaaatattt ttttgaggcc gtgtatcgta
    26641 tccataatcc tatcctttcc tgattattca gatcgctgta gatgggcatt tattatcgct
    26701 gaacagaacg ggtccagcgt tttccattcg cttcaaactc aacggtatcc cgcgaaatcc
    26761 ggactactgt aacgccatta atcgtctgac cttcggtcac tacttgaccg ttgactatgg
    26821 ccgacggctt gtgctggcta aacagaatgc cgcgcaccgt cagatcgccc acttcggcat
    26881 cagtatcagc tgtctgcaca ggcaccgcct gacggacaac cggtaccgtc gcatcgcgaa
    26941 ggtcattggg aaccgggtcg ggacgcttcc agtcttcaat agttttctgc aaagccggca
    27001 cactctgttc aggatccgga ctgcttacag cggcaatggc tttgcctttt cccagcccgc
    27061 ccaaggtcac atacaataca gcagcaaaca ccactgataa aatgccgact aaaacagcca
    27121 tttttttctg acgcgaatca acttgtcctt tagctgagcc gaaaacagct ttcttcacct
    27181 gcgaagaaaa accgccgcta cctttgggtg ctgatggaac tgctgacttc ggcaagggca
    27241 tcggccgttt ggacgaaacc gccggcgtac tgatacagac cgatggatcg gcgctgaggc
    27301 gctgggccag cgtaggtctg gatgcagcag acgcctccaa ggaatgggat gcggctgccg
    27361 tcggcggtac ctgactgggc ggcgatgcag ctgcagagag gctgttttga ggcatcggca
    27421 cgccgtcaaa tatggacgaa atctgcttgt gtagtcctga ctttctcata ggttatctct
    27481 tccgctaatt gcctgtattc ttttgcaccc ggattggccg gatcgtaagt gatgatggat
    27541 tggcccgctg ccggggcctc tgccaatcga atattacggt gaatgaccgt ttgaaaaata
    27601 agctgaccga aataatcccg catctgccgt tccacatcac gtgagagaat tgcatttttt
    27661 tcggccaagg tcagcaaaat gccccgcact tgaagctggg gattgaagcg ttcctgaact
    27721 acactgaggg tttccagaac ctgttttagt ccttcgatgg aataataatg cgtctgaaca
    27781 ggaataatga catcggaagc cgcattgagg gcgttaagcg tcaacaaact gagagaagga
    27841 gaacaatcga taatgcagta atcgaaaaac aggtcagcct gaagaaggcg attatacaga
    27901 acatattcgc gctggttcat tgtcatcagg gctgcttcgg cgcctgacag aagcacattg
    27961 ctgggagcaa tcatcagcag cggtatgccg gaaggcatca gaatacgcga taccggcaaa
    28021 tcatcctgaa caatgacatc aaagattgta tgctgacagt gctccggaat cagtcccaat
    28081 ccgagagtgg catggccctg aggatccaga tcaactaaaa gcgtgcgctg cccccggaca
    28141 gccagtgcag cagccaaatt gactgctgtt gtggtcttgc cgcagccgcc cttctgattt
    28201 gttattgcaa tggttctcac gatgccgtat tttgccttta attcttaaga gtcttatctg
    28261 ctgcggttag gccggctgcc gatgcaaccg gttcagcgga gggctgagca gatgccaaaa
    28321 acgacagctc cagcttgata tcatgacctt tggattccgc tgttgcccgc cgaaactgga
    28381 gtttttccac aaacaccgca gggcagttcc gctccagctg gttgatgaag cggacaaact
    28441 gttcaaatga tgcaaaaaat tccaccgaca gccatccttc ggacagccgc ttggtacttc
    28501 cgagcgtcga agcgtaggaa gtctttgggt tcttgctgga aaaatcaatt aggcgcagat
    28561 catttgaaat ctggccgatt tgaaaaacaa gattggttac accgtcagcc ggtacagaaa
    28621 agtccgcaat agtccggctg gactgctcca atcgctgctt gagctgctgt tttgtttctt
    28681 ctcgggctgc caggcgggct tgttccagct gctggctgct ttcggaaaac tgccgctgga
    28741 cttcaatccg ctcggcctga agcggcagat accactgcca ataccccaag accaagagga
    28801 cgatgcagag gctccataca gcaataagca ttctggcata ccggctgagt tttatttttt
    28861 tcagcttcat gatcatccct gctccttaag aacgcattca atttcataat agacggcatt
    28921 ccgattgtcc acctgcccct gctggcgggc tgtcggcttg atttgctcaa atacagacct
    28981 aagacgcgcc gactgcttga ggcggtcgag atattcctga acagccggat cgcttcgctg
    29041 ggcatcaaaa ccgcagagaa tcagattcag ttttcggcgg acgaccagcc gctgcttggc
    29101 atcggctgaa tcggcctgct ggattttttc aacaacagaa ttccggtcca gcttcatctc
    29161 atagagaaag atgctgtccg gcagggtttg tacaagttcg accagcagat ccgaaacctg
    29221 aattcgatag gccagggctg cagagatgtc tttcaagcat ccctgcagac tattaatctg
    29281 ggcgagggtt ttgctgtatg cggcagcatc ctcggcatac aaggccatct gctcctgatg
    29341 cgtctggagg gcggctttct gggattccag aagcgctttg ctatgctgcc atgaagcggc
    29401 cagcccccct acggccaaaa tcggaatcag taaaggaact gctttaagac caatccgctt
    29461 cagatcggtc ttttccggca atccctttcc tttgagcaaa tcgatagaaa acatcattcg
    29521 accatcctca ttgcaagacc tgcggccaca gcaaatgccg gaccgctttg aatcagttcg
    29581 gctgcgttgg gaatagcatc atcaacaggc atggcctcaa acggatttag aatatgtacc
    29641 ggcgccggca gagcgtcagc aagaaattcc acaaaagacc ccgtttgcgc caatccaccg
    29701 gataaataaa tgctgtccag gccagcgcct ttttcctgaa acgaatagaa ccgcagggtc
    29761 tcgttaatcg cgttggccag cggccggatc gcatcattta aagccagcag caccttattc
    29821 tgcggcacct ccggtgaggg ttcgccggcc agaaaccggc aggcctggct ttcggaaacc
    29881 gcaagcattt gactgagctg ctcggcaatc cgctgcccgc cgatatgaat atcgcgcaca
    29941 aacggcaggc cgtcctgacc gtaaatgacg acattggtga acatcatacc catatcgacc
    30001 actgctgccg ttccgccggt ttcgagcaac tgcagctcat tgagacaatt cagaagggcc
    30061 aatgcatcgg catcaatcat caggacccga acgcctgcct cagcaagaat ctgcattttc
    30121 tcttgaagta catgttcggc ggcaaccgcc atcacaccat gtttcggctg gacagtacgg
    30181 ccgtcagggg acactatcgg ctgactttcg ataagctgat agtccagcac actatgcttc
    30241 atatcaagcg gacagacctg ctgggcttca atggcgactg cctgaccaac agcttccatc
    30301 ggcagcggtc ctaatttaaa gccccgtatc atcacttccg ggcctgaaac accggtcaca
    30361 gcacttcggc ttttcaggcc ggccttttga aagcaggcct taaccgcttc ggcacaactc
    30421 agacgacgct gtttttcgtc tcctgtgccg ccgtcaatcg gctgtctgac agccgccgca
    30481 agagcatacc catcactggt tttgcgcagg cggaccagct tgaccgcgga cgttccgatg
    30541 tccagaccag ccggttcaat ttgtcgatgc gtcagtaaat tgagtgccat acttttgttc
    30601 cgttattctt cattccagcg gctgatgaca aatcgagcac cttcatccgt tacagaaacc
    30661 gtcttgagtg cagcgtcata gtaaaaattt gccggacttt tcaattcgaa attattggcc
    30721 acaaaacttc cgtaaatatt gcccccggaa tagaccgtca aatccgcatt tggcgcataa
    30781 ataacgccaa atgcatcggt tttggccttc aaatcgattt tttggccaat cggccctgtc
    30841 ccgtacagct taaaacgcga gggaatctgg atggcgttat tgatgcctga attgttgtcg
    30901 gaaatccagt tgccgtccaa atagaccgtc aaggaggcat tataagccgg atccagaaga
    30961 atttcgctgt tttgccccat atccacagtg ccggttatat acaacgtgca gtcaccgata
    31021 acccgcaggg tagcaccttg ccccaatttg acagaggaaa accggccgcc ggcgggaaaa
    31081 tcaccgcccg gtccgattgt tttttctcct gttttaacct cgatttttgt attgggtccc
    31141 agcagcggcg gcggggaaac ctgcggaaaa tcgagggttg aagacagtgc atagctgcgg
    31201 tcaaaaatgg caccctgatc tttgatgact ctggctacat cgccgccgaa tcctaccaca
    31261 acatcgccat ctacaacgac gttattgttc agaataattg agcaggcctc tgtactgttg
    31321 gtcccgataa taactttctg actgcaatcg tccggattta atgagacgtt tgaatcaacg
    31381 ctttgaacga ctgtaccgct tttaagaata atattgtttc ggcattgaat tgcattatca
    31441 aacagacctt tgagccgcaa agcggctctg actgtacggc tgcgattgcg gtcgatacca
    31501 gtagatgcta tgcgatagcc atcggcagaa tcataggtcg ttgttacgga ataaatagca
    31561 ttggaaaaag gcatttcgac gccgctggcc gaagggagca gatcagcaga ccaagtttta
    31621 gcgatcactg cattattgat ctcttggacc gcacgttcta ccccggcgtc agcggccaga
    31681 cgggcttcca tatcttgtat ctggtcaagg gatgccatgc gtgactgtgt tccaagcagt
    31741 aaaacgctgg ttccgataat aaaacagacc atcacaatca ccagtacaag gaccaaaaca
    31801 gagccggtct gttctttttg aggcaatatg ttctgtctta cagttttcat ttatcggact
    31861 ttctgaatct tcaatacatt ttctaaccag gtatcggcct ggatgcagga tgacttaacc
    31921 atcattgtgc aaaatagccg tcgtaaccat tacagtcgat tcgtggtcat cctgcagaac
    31981 caatttcatt tcaatagcac cgttaagcgg tcggaattcc aaatctgcca ccgtggaagc
    32041 cagacaaact tccgacaaaa cttgacctgt gcccacatcg ccgtattgaa catagaggca
    32101 ggatggatca gcagaattac ggtagaatcg ggcaaaacgg tccaattggg ggcttgccag
    32161 ccaatcgctg taataaaaaa ctgtaatatc atcaattgca tcgaaataat acagcgtgcg
    32221 ggatgctttg cgcacaacct tgtcaaaggc cgttttggct acggcagcat ccgcaatcgc
    32281 cctgctgttg acttttgcat acgaatccag ccagccccgc tgtgtatcca gcatcactac
    32341 ggcaacagcc agcaggataa tagaactgat cgctatggct aaagtcagtt ctgttagaga
    32401 aaatccggct ttcattggca tggatttcat aa
//
LOCUS       DAOTBO010000024        28658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1351_length_28658_cov_5.108450, whole genome shotgun sequence.
ACCESSION   DAOTBO010000024 DAOTBO010000000
VERSION     DAOTBO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 28658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28658
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_28658_cov_5.108450"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLE88_04745"
     CDS             complement(<1..817)
                     /locus_tag="PLE88_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75810.1"
                     /translation="MARERWGSKLGVILAVAGSAVGLGNFLRFPGVAASNGGGAFMVP
                     YLVSFFLLGLPLMWIEWTIGRFGGGFEHSTAPGIFDTVWRKSRFIKYFGVIGIFGPVV
                     IFSYYTYIESWLLGYSFFALTGKYAACTNQQAMASFLKGYQGAAANEYFSGLGTAYLF
                     FVITFILNVAVVGFGVNRGIERFCKWALPLLLVIACVLVVRVLTLGTPDPTRPENSIL
                     NGLGFLWNPDWSALAKGHVWLAAAGQIFFTLSVGIGVILTYASYLTKQDDVALS"
     gene            complement(930..1706)
                     /locus_tag="PLE88_04750"
     CDS             complement(930..1706)
                     /locus_tag="PLE88_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOM75811.1"
                     /translation="MIIDCHTHLYSSQRGNLAQEHLEICQRLDGCFVMAGLSDDRQSV
                     NQDLVDYVSGSSKAYGFATYNPVQDDVSLKGVRKATRDRGLCGVVFYCAENAFHPAHS
                     RAMQFYESAEELGLIVFFHNCPPYSPQSVLDFAQPWLLDEVARRFPNLKIIIGRMGMP
                     FYWQTEGLLAKHKNIYADLSVHPQRIWQTYNLVLSAYEAGIMDKLLFGSGFPFARPDA
                     CIETLLGFNRLLADTHLPQVPREKLRSIVERDTLKLFGLK"
     gene            1925..2341
                     /locus_tag="PLE88_04755"
     CDS             1925..2341
                     /locus_tag="PLE88_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOM75812.1"
                     /translation="MDCPICKTAMIVLELEEVEVDYCPQCVGIWLDAGELELLLDDAH
                     KAKELLESFKAAKSDEKKRKCPICRKTMEKVLVGQHDSKQELIDRCPRQHGLWFDRGE
                     LQAVLTMGRFDDAGRIQRLLGDLFCPEAKNPQKLSQ"
     gene            2676..3161
                     /locus_tag="PLE88_04760"
     CDS             2676..3161
                     /locus_tag="PLE88_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOM75813.1"
                     /translation="MRCPGCKGLLRAERHDYIEFHICSHCRGIWVAGEQFHALAVRVA
                     AEGQVPSSAKLTFEPRKALKPAEDNPARLCPVCDKPMQEFSYAYDSNIFLDRCRQCRG
                     IWLDPNEIIDIAAHIQYNPDVDAIGRLLIKDENAQDETQWLYMLAAAALAVLRAVLFK
                     R"
     gene            3276..3851
                     /locus_tag="PLE88_04765"
     CDS             3276..3851
                     /locus_tag="PLE88_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOM75814.1"
                     /translation="MILSAGLIGMLVILGVVVLLVLFVIGVYNSLVRLRNQVDNAWSQ
                     IDVQLKRRHDLIPNLVETAKGYMQHERGTFEAITEARSRAMGAKSVAEASKAEGALTE
                     ALSKFMLVVENYPDLKANQNFLSLQETLTSTENKIAFARQGYNDQVLFYNNKIQMFPS
                     NIIAGMFNFTKRDYFEIENEAERSVPKVSFS"
     gene            3955..5556
                     /locus_tag="PLE88_04770"
     CDS             3955..5556
                     /locus_tag="PLE88_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HOM75815.1"
                     /translation="MRELIRINRRNSILLQALVMNVISGLCGRIGISECLASGMAQEA
                     PRAARSVHLWYPAPEGMVYYNELTVEQSAPGSYFCVCGFKNGYFGIQEISPERKVVIF
                     SVWDPGNQDNPDMVPEDQRVKVLYAGQGIRIKRFGNEGTGGQSMFDYDWKIGQRYRCM
                     VKAAVQDQRTAYAAFFYLPEQQTWKHLATFETITGGKSLQGYYSFIEDFRRNGQSAQQ
                     IRRARYGNGWIKTVDGQWIALSKAVFTADNNPSIHIDAGRIEGGFFLQNGGETKNSTP
                     LSSSLTCSTDSFEQPEDPQWKLIWNDEFNGQGLVDEEKWNYEEGFIRNNESQYYTRRR
                     PENARQENGTLVIESRKEAFKEGKYTSASIHTSGKQSFLYGRFEVRAKLPTGNGTWPA
                     IWMLGDSIHQVGWPACGEIDIMENVGYDPETIHANIHTKAYNHVLKTGKGSKIRADKP
                     WEQFHLYAVEWYEDHLDFFMDNHKYFTFQNEGTGPDVWPYDRAHYLILNTAIGGAWGG
                     QQGIDDSIFPQKFLIDYVRVYQQQN"
     gene            5578..>6003
                     /locus_tag="PLE88_04775"
     CDS             5578..>6003
                     /locus_tag="PLE88_04775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOM75816.1"
                     /translation="MWELIRINKRNSILLLAAMGAVLLLLGYILGVLFGGNPQAGWMG
                     MTAACCIWLVLMLISLSSGDQILLASSRAVPVTADVHPMLFNVVEEMTIAANLPKMPK
                     IYIINDPAPNAFATGRSPQSASVAVTAGLLARLNRDELQG"
     assembly_gap    6004..6013
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6014..6801
                     /locus_tag="PLE88_04780"
     CDS             <6014..6801
                     /locus_tag="PLE88_04780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOM75817.1"
                     /translation="HIVHRDILFVTLAGVMLGSIVLISEIFMRSLFYGSLGSQRRYSS
                     RSSNQGGGQLIMFVVAILFAILAPIFAQLLYFALSRRREYLADAGAVRLTRYPEGLAS
                     ALEKIAGCPINMESANKVTAPMYIANPFRGRKAVHLYSTHPPIEERIRILRNMSHGAG
                     YIDYEKSYEAITKRSPVVPMSALKEEAVAIRQGAAEPAAGPTARQQARRLGDLMRTVS
                     AFAFLTCPGCGLKMKIPPEYKGSRVECPRCRHIAVLKQQQTNA"
     gene            complement(6846..7493)
                     /locus_tag="PLE88_04785"
     CDS             complement(6846..7493)
                     /locus_tag="PLE88_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75818.1"
                     /translation="MKSGIILLSAAVLLLGASSPVFSYPAPAIVQPIGQWTLQVKYSQ
                     PEQLLVKLAGKEAPQRFWYIILTVTNNSSFDTVDFYPVCQLVTDTFQIIDADKKVPKA
                     VFDVIARKHQGSYPFLESLDFVEHRIFAGEDNTRDFAIIWPDFDPKARQISLFIGGLS
                     NETAVVLHPVQKDPQGNPVKIFMQKTLQLKYSIGGDERLRDTAALKEIESRWVMR"
     gene            7661..8575
                     /locus_tag="PLE88_04790"
     CDS             7661..8575
                     /locus_tag="PLE88_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOM75819.1"
                     /translation="MLKKYTIENGKLIENGRDDAQVHVYIAPDEAEKRYLIDQLKIDE
                     HTLNSSLDPDELSRLEFEPEHIAIIFKRPRHYQASDDFLFKVLSAGVFVFKDRLIIVM
                     GEEAPLFEGKPFIKIQTIQDVILKLLYRSISHFVEHLKGIDAMANEIEKQVNVSMENK
                     YLINMFTLEKSLVYYLNGIISNGVLIEKLKNNAVRIGFSQENIEFLDDILIENNQCKG
                     QADIYSQVLSSLMDARASIVSNNLNIRMKQLTIITIAIMLPTFVVSLFSMNVRIPLSQ
                     YHNAFWIISAMAAMTTTIVAVLWWKRRW"
     gene            8930..10066
                     /locus_tag="PLE88_04795"
     CDS             8930..10066
                     /locus_tag="PLE88_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOM75820.1"
                     /translation="MKNQPLDTHVQTETNKTPEIEKLFRAVIKADASDLHLKVNMPPK
                     IRVHSKLKSTTGDPLTEEKIERMVFEIMSEDQKQFFLKNGALDFAYQVGDMDRFRINV
                     FRQRSYISLAARRISGRIPPFESLHLPPIVEKIAESHDGLILVVGPTGCGKSTTIASM
                     IDHINRLYACHIVTIEDPIEFLHVDKKAIVSQREIGIDVPSYEEALRSLMRQDPDVVL
                     VGEMRDQETLTAAMRAAETGHLVFGTLHSNNCSQTIQRILDLFPQEERDLMRQTFALT
                     IRAIIAQQLLPGIRPDVPRVPAVEIMINTPIIRKLISDQREADLPTAIRAGEGEGMQD
                     FTESLRRLVEEEFIDLKVALQYAPNVEELKMALKGIRSTASGIL"
     gene            10105..11742
                     /locus_tag="PLE88_04800"
     CDS             10105..11742
                     /locus_tag="PLE88_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOM75821.1"
                     /translation="MPMDVIASVDYGGYVSLWKLLFFMLAFVLWAPMVNWVFTDSQAV
                     RTNTLVWTLTIALAGIGALFVWLLIPVFIIGLLLYLLVFAGASMAYVIHRNSRVAEFE
                     KVLTSEHIRGLLVNPAKKMEKASRGITFITANGNEVPLPEPKSREAEGFILACEVIDD
                     AVYHRADVVRFVPQKEDYALLYEIDGVTTQQNPRTREDIDHFVYYVKELAGLEVEEKR
                     KPQKGRFTAVLPDNKRTSWEVSTSGSTAGEQIRLERISELVSRKIADLGLNDNQFESI
                     NSLRDIKSGLVIISGPPKSGVTTTFYTLLGNHDPFMNNINTLEKNPAAELPNITQVQF
                     TLGDSGENTYARRLQSVLRRGPDIIGVADCEDDQTAKLCTAAAKDGKIVYVCISATSV
                     SDAMDKWCGWVGDKGLIADTLEAVINQRLVRRLCMECRQAYQPNPALFKKFNLPPDDA
                     RMFYRPGEIEYDKHGKPIVCKNCQGTGFFGRVGLFETIRITPELRDAIRKAKTVQEIT
                     TAFRRAGMLYMQEQSIKKVSESITSINEVIRNFSAKS"
     gene            11776..12879
                     /locus_tag="PLE88_04805"
     CDS             11776..12879
                     /locus_tag="PLE88_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75822.1"
                     /translation="MELAIIVIGILLTMAYFYLKCTPMSSLSTLLASIFALIIAFSWY
                     EKITELFISRGYGVDWAHFGCFLLSFILTFALLRALADLLVGVNIDLGKPVKLITALV
                     CGFLTGIILTGTLLVALGMLPLQGKVFYSRFDPSKPVSLNSPKVPLLNADGFVVGLYR
                     LVSAGSMSSGKSFGVVHADFLKQIHLNRLRVQEGVLTVSSRKALILPSGKNQKPVREW
                     DIPNRGKLTVVRMGIVARNIADGGANNSSGQIQFFPGQIRMICKPSGTDAKKMLAGSG
                     KAIWPVGFIEGGKLVEKHLEEIITPETKGLKDRTIWLDAAFDVPQGQEGVLLQFKQYA
                     MVQLPKAVSTSDEVEKALLEGSAEANAESSPTE"
     gene            complement(12980..13129)
                     /locus_tag="PLE88_04810"
     CDS             complement(12980..13129)
                     /locus_tag="PLE88_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75823.1"
                     /translation="MSKHEIIDCILEINKSAKPEFLEQFSVEDLDLYLEHLMQVDLEE
                     VCLCA"
     gene            13262..14455
                     /locus_tag="PLE88_04815"
     CDS             13262..14455
                     /locus_tag="PLE88_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75824.1"
                     /translation="MTAEPLTHTILKVYLSALGASNKTLKSRGKIEPLNLEIFHSAGY
                     NNQKELSGKNYCGINMDLQDNAFNAGRMRHQPQLKLWRYAGLMLTYRCTAACRFCYYY
                     CSPQAGGLMTADTAIAAWQGLIRIAGKDARVHITGGEPFLCFDRLAEICRLACRLGLT
                     PIDSIETNGFCSGSDKELSEKLQFLNDCGLERLKISWDPFHEEYIDIEQVKRTVFIAR
                     RILGPQRVLVRWEKYLSAPSGIRQQSQAQRQAVLQRALADDVCRFTGRAAEALAHLAP
                     QQPLTQFSDKNCRNALLSAKGVHIDPDGNVFVGQCSGMILGNAGRTPLDVIWKNWQPD
                     RGDFWGVLYDKGPFGFLEKAQKQGFHPRSQYASKCHLCTDIRRFFFDNQFFSTIIGPS
                     DCYGR"
     gene            14440..15717
                     /locus_tag="PLE88_04820"
     CDS             14440..15717
                     /locus_tag="PLE88_04820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOM75825.1"
                     /translation="MLWQIENADWRFVLAAGDTTSYDTIVGRMVVEQRLCTEEEVRRC
                     LEELKERASSRPATLEQLLLEKQFLTPSQAARLQSMIRESKDVSSQIPGYKILGKLGS
                     GAMAVVYKAKQISLDRTVAIKVLPKKFVQKSDYVERFYKEGRIAAKMNHNNIVQAIDV
                     GEVGGLYYFVMEYVEGRTLYDDLSKGKIFSEKEAIDIILQLASALGHAHSLGLVHRDV
                     KPKNIMISKDGVVKLADMGLARETTDVKAAKHEQGKAFGTPYYIAPEQIRGLVDIDGR
                     ADIYALGATLFHMVTGRVPFEASTPSEVMRKHLKEPLTPPDHINTSLTAGISEVIEVM
                     MAKEREERYRNMEEVIADLQAVRDGKPPLLARQKFDVEALEQLEEGMAIETTAGTERV
                     YTEEMIAKYRIGLLALGILSAILLLVILFLVMR"
     gene            15755..16360
                     /locus_tag="PLE88_04825"
     CDS             15755..16360
                     /locus_tag="PLE88_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOM75826.1"
                     /translation="MSRRPTDTRERILDTATNLFSSHGFASTSIDDILKAVGITKGAF
                     YHYFNSKEHLCQVILDQAVCVFHQLAESLLESPDSAWPLRQWIEMLARKQQSGQWLFD
                     RLLYRLTVESSQLSPEAQNQLRMFWQWCQVFYESLIRKTIGEKGDWPKPSSSARLLAA
                     ALFGAVWLDRCAGSDEEQGAIRQALLELIAAAQADPAKTAE"
     gene            complement(16394..16464)
                     /locus_tag="PLE88_04830"
     tRNA            complement(16394..16464)
                     /locus_tag="PLE88_04830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16430..16432),aa:Ala,seq:cgc)
     gene            complement(16550..17749)
                     /locus_tag="PLE88_04835"
     CDS             complement(16550..17749)
                     /locus_tag="PLE88_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM75827.1"
                     /translation="MRTIAVINQKGGCGKTTVSINLSAALAGRGYRTLLMDMDPQGHC
                     AVGLAVPEEQIEQSIYDVLIGMGRGEPMRLKEILWQICDRFELAPSSIDLATFEQQMN
                     GVKERENCLRKVLDAVREEYDYVVIDCPPSVGLLTFNALRAATDVIVPVEMGYFSLHG
                     LSRQLETLNILCQQCEQKINVMVLASMYDIRTKMGREILAELKKHFSERMFKTVVNFN
                     TKLKEAASLGQPISEYDPACKGYKDFLALADELIGADTQMHRANLVNSLQARLSTISA
                     SAQELLESTGPAKQEEKAQPALSFNEKLADFYGVRQIDGQVVFSTLYPRAQTVQIAGD
                     FNGWDPQQTSFQKVGDTGKWVLSLPLDRGVYRYRLVVDGQWQQDPYNGQTEMNPFGDY
                     NSVLYVQ"
     gene            18080..18283
                     /locus_tag="PLE88_04840"
     CDS             18080..18283
                     /locus_tag="PLE88_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75828.1"
                     /translation="MTEEQIVKWMRERVQKNGFTDATSLAESFLKEYKISDALDPIFS
                     RSLDAGFKVAQEIRDRRLALQPN"
     gene            complement(18454..19764)
                     /gene="dnaA"
                     /locus_tag="PLE88_04845"
     CDS             complement(18454..19764)
                     /gene="dnaA"
                     /locus_tag="PLE88_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOM75829.1"
                     /translation="MAAQNSTEILDGILERVKAIDPVNTRTWFDNLHIAAFDHGLVQI
                     QCPDQSCAEFLQDRCQGTFTQAAQAVTGHLVSVQFLYNGSPHKKPTLPAEAAGRLRLH
                     PDYTFENFVVGPCNRLAHASCVAVSQNPGVIYNPLFLYGSAGLGKTHLLHAVCHDTRK
                     KQADISIRFLSCEEFVNGFISAIEKGTLSEFQSLHRTVDVLIIDDVQFLREREQSQEE
                     FFHTFNALYNNRRQIVLTSDSAPQDLAALEERLISRFKWGLVARLDPPSYETRIAIVK
                     KKASLRGLELPDGVAELVAEHIKNNIRELEGALTMIYAMSKTAGVPITVELARQALGV
                     QGADAAKNITISEIVRAVSEQFDVRIADIQGKKRSQSIALPRQVCMYLARCLTRHSLE
                     EIGGHLGGRDHATVMHACAKIEQMYKTDAQFRRRIDTLISRIKP"
     gene            complement(20043..21611)
                     /locus_tag="PLE88_04850"
     CDS             complement(20043..21611)
                     /locus_tag="PLE88_04850"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOM75830.1"
                     /translation="MKCPKHSSIIFFCLVWQSLSFGTALDDYVASPDSSYSWIQVGSS
                     WFDGSQWTVSTTAVNLRLTSQTWRSSSEVTPSVWTHWVTVIVPGGLLLGSVKNTALVL
                     INGGSITDPAPTLDPSDPVNRQFRDLALATRSVIVDLRGVPNQPLVFADEGFGRSEDE
                     IIAYSWEKFLSGGDLYWPVQLPMVKSVVACMNAVQAFRPAVKNFVLTGGSKRGWTAWL
                     TAAVDSRVAAIAPIVSDLLNMRRSFAHHWSCYGFWAEALHPYEEMGIFNWFEHPRTSE
                     LMSIVDPYEYRSRLGIPKFIVTAAGDDFFVHDSIQFYIDDLVGETFVRTMPNTNHYLD
                     GAYEQVVANMVPYYDAFLRGQARPSFTWTTQPDGSMAIQTSATPLSVTLWQADNPAAR
                     DFRRVTIGDAWIPTPLTAVSSGLYIADPGVPPTGWRSYFVELVFDFSGSFLGQLDDYD
                     YHFTTEMRVLPEVRPFEADFTRDRYIDTEDLIILADNWLTDTPYYDIIPRRSGDGLLN
                     LGDFTAFGLHWLTP"
     gene            complement(21738..22280)
                     /gene="msrB"
                     /locus_tag="PLE88_04855"
     CDS             complement(21738..22280)
                     /gene="msrB"
                     /locus_tag="PLE88_04855"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOM75831.1"
                     /translation="MNLFYMQIVYIALLTALAAFMKGCPAEPSREIQPTSAAIQKEIP
                     MDANETPFFTKTDQQWKKILTPEQYYVLRQKGTERPFSGKYDLHFEKGIYKCAACGNP
                     LFGSEAKYNSGCGWPAFWTPTNPDAVAESRDTSHGMIRTEITCSRCGSHLGHVFNDGP
                     APTGLRYCINSAALNFEKNP"
     gene            complement(22284..22409)
                     /locus_tag="PLE88_04860"
     CDS             complement(22284..22409)
                     /locus_tag="PLE88_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75832.1"
                     /translation="MGFLKAIGTIVENSHIVNSGDLKQLDYFCMALPQRAVWLNR"
     gene            22461..23510
                     /locus_tag="PLE88_04865"
     CDS             22461..23510
                     /locus_tag="PLE88_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4056 domain-containing protein"
                     /protein_id="HOM75833.1"
                     /translation="MTRKGIFGKASLVAVLAAILLAGCQTAGKPRMRIGSFFGDAAGL
                     TFVDPNHLGPHSRRHNFHEKSGLLYTCGAGFIDLGHLREAADRTEYLARITYQNLLQQ
                     DQSFSYRLIEPARYEVKVIYPPGWASLSQLEKERIASEAAIIFGQYGAHTSLIWHEIL
                     TWYGFASSGIFSENISSFSWEDPYSDVLGTHLAVEAMRNPAPYEEAMTRLISQKLAQL
                     DVQPISVAQEAARKVAGRWYTGGYYFFVEMKRRNFDVGFDDDLLTPWLVPEMCPDAVP
                     QPCPRPTLEPLAAWGFCFELALYPAEFEKTKIFKALGRKKAVIYPLEDFPLLIEHIRA
                     EAQKTYGPQIDCPVF"
     gene            23545..24615
                     /locus_tag="PLE88_04870"
     CDS             23545..24615
                     /locus_tag="PLE88_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4056 domain-containing protein"
                     /protein_id="HOM75834.1"
                     /translation="MTQKYRLDKLTGLTLLLQKAAVCLGIAALLTAGSGCGLTGLTGP
                     RARLGYVPAATVGVVFADPDNLGSHAAVYSPFENSGLVYTCGAGVLDIDHIRGSADLT
                     RYFIGRVREALTKGQQQFSYKVSGETSRHYITIAYPPDWETHPDKQRIIEEIAFEAGP
                     YLAFDATIWHEILTWFGVHFMGFEPEFNSAFSYEDLYSNLLGCRLAVDALQDEQNDYD
                     RAMTQRLNAVLQELGVQPRQIAVEAVKSVEGRWYTGGLIPDMKMRNFDIGLDGFITPV
                     LIENVKNCPDARAVPLPAPALERLQQYGFSLTHEIKPNVFEQGAIFKAAGSKRIVPDE
                     HYPVLIEFMKQDAARRGYQYSD"
     gene            24682..27219
                     /locus_tag="PLE88_04875"
     CDS             24682..27219
                     /locus_tag="PLE88_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM75835.1"
                     /translation="MRLYLIYGLVLYSQLAAGQTYYADSRQGNDAADGTSPQTAWKTL
                     EKMNSIVFEPGDKLLLAAGSVFEGQLKPQGAGALIDGTPAAVCIDSYGDGPKPRIDAQ
                     GRYHSALYLYNTQYWDIANLELTNTGSDRQSGRCGVYVHIRDFGTAHSIHLRHLYIHD
                     VNGSLIKNQGAGQGILWRNEGRRRPSCFDDLLIENCRLVRCERNGIIGAGYSRRDSGK
                     LFTPSLRVVIRGNLLEEIPGDGIVPLGCDGALVERNVMRRCTRLLPEGEAAAGIWPWA
                     CDNTIIQYNEVSGHKAPWDGQGFDSDWDCTNTLIQYNYSHDNEGGFLLICNNGGASPA
                     TAVNTGTIVRYNISVNDGLRTQPTREGMFSPTFHISGPVKNTKIYNNVIIVPKKPDAR
                     IDATLIKMDNWGGPWPEDTLFANNIFYVEGQTRCDWGGSIGHSFSNNLFFGTFVSRPN
                     DPSGISADPKFAGPLFGGDGFEGLTAYRLKPDSPCIDAGIPVGGTADFWGNPLSSRPD
                     IGVYEKQPDTPQPSAEIQPLPFVDIHDPHIIRHKDFYYIYSTGRRGQVIGAIRSNDLK
                     RWYRIDSPLAAVPDWVTEQIPRCRSLWASDVHLINGRYCLYYSASTFGSQRSMIGMLS
                     NTTLDPADAAYKWVDEGKIIESDRGKDFNAIDAAVAADEQGRVWLCFGSFWGGIKMIE
                     LDAGTGKALGDNPTVYSLARRQQGTSAIEAPYIIRRGPWYYLFVSFDACCRGARSTYN
                     IRVGRSENVLGPYRDADGIPMLDGGGTLVLEGGQRYKGPGHNSILQDGGTDYLVYHTY
                     DAQNRGMSILQIRPLVWDSDGWPHPEAPLECVNRTPAWDAPRRRMAE"
     gene            complement(27397..>28658)
                     /locus_tag="PLE88_04880"
     CDS             complement(27397..>28658)
                     /locus_tag="PLE88_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM75836.1"
                     /translation="RKRTFKLQQLNKRLRHKMAECRQAEEQARVLAAEAVAANRTKSE
                     FLANMSHELRTPMNAIMGFTEVLSDDTLTQEQRCYVQMISTSSKGLLALINDILDYSK
                     IDAGRMVIEICDCRIGELLTEVESMLRPQAMQKGIRFEVLQCDMVPETIKTDPLRLRQ
                     CLINLIGNAIKFTEAGHVYVNVACIEDEGQIWVRFDIEDTGIGIPKDKQMIIFDSFTQ
                     ADSATTRKYGGTGLGLAITKRLAELMGGSVRVVSQVGVGSVFTLTIPAGVKTPEAVWN
                     KYKQIDELNEMMDTMKGTAMYIGKALVAEDNPSNQKLMQILLQKLGLEVVLADDGVGA
                     VEKGASGSYDIILMDMQMPNMNGYDATRQLRSRGVKTPIIAVTANAMCGDEQKCLDAG
                     CDGYLSKPIDRTKLNEIIGQFIPAHAS"
BASE COUNT         6964 a         7338 c         7760 g         6586 t
ORIGIN      
        1 ccgacagagc cacatcgtcc tgcttggtca gatagctggc ataggtcagg atcacgccga
       61 taccgacgct gagggtaaaa aagatttgtc cggccgcggc cagccagaca tggccttttg
      121 ccagtgcgct ccaatcggga ttccacagaa agcccaagcc gttgagaatg gaattttccg
      181 gacgggttgg atcgggcgta cccagcgtca gaacccgcac aactagcaca caggcaatga
      241 ccagcagcag cggcagcgcc cacttgcaaa aacgctcgat gccccggtta acgccgaagc
      301 ccacgacggc cacattaagg ataaaagtaa tgacaaaaaa taggtaagcg gttcccagcc
      361 cggagaaata ctcattggcc gcagcgccct gataaccctt gagaaaagag gccattgcct
      421 gttggttggt gcaggcggcg tatttgccag tcagggcgaa aaagctgtag cccagcagcc
      481 atgattcgat gtaggtataa taggaaaaga taaccaccgg tccaaagatg ccgatgacgc
      541 cgaaatattt aatgaagcgg cttttccgcc agacggtatc gaagatgccg ggggcggtgc
      601 tgtgctcaaa accgccgccg aagcggccga tggtccattc gatccacatc agcggcagtc
      661 ccagaaggaa gaaggacacc agataaggaa ccataaaggc gccgccgccg ttgctggcgg
      721 ctacccccgg aaagcgcaaa aagtttccca gacctaccgc actgccggca acggccagaa
      781 tcacgcccaa tttagagccc cagcgttctc gtgccattat atgttttccg cgtgaataaa
      841 tatattagac aacaaaccgc agcatcctgc cccgatgcgg ctgaagcaat ctgcgtctct
      901 taaaaagcgg ccgcttttgc aaaaacaagc tatttcagcc caaacaactt caaggtatcc
      961 cgttcgacga tactgcgcag tttttcacgg ggtacctgcg gcagatgcgt atcggccagc
     1021 agtcgattga agcccagcag cgtttcaata cacgcatccg gccgggcaaa ggggaaaccg
     1081 ctgccgaaca gcagcttgtc cataatgccg gcttcatagg cgctgagaac cagattatag
     1141 gtctgccaaa tccgctgcgg gtggacgctc aggtcagcat aaatattttt gtgtttggct
     1201 aaaagtccct ctgtctgcca gtagaacggc attcccatcc gtccgatgat tattttcaaa
     1261 ttcggaaacc ggcgggcgac ttcatccaaa agccacggct gtgcaaaatc aagaacggat
     1321 tgcggagaat acggcgggca attgtggaaa aagacaatca gccccagctc ctcagcagat
     1381 tcgtagaact gcatagcgcg gctatgggcc ggatgaaagg cgttttcggc gcagtaaaag
     1441 acaacaccgc acaatccgcg gtcacgggtt gctttgcgga ctcccttcag ggatacatca
     1501 tcctgcacgg gattgtaggt tgcaaatcca taagccttgg aactgccgga gacataatca
     1561 accaaatcct gattgactga ctggcggtca tccgacaagc cggccataac aaagcagccg
     1621 tccagccgct ggcaaatctc taaatgctcc tgtgccagat tgcctcgctg cgaagaatat
     1681 aaatgggtat ggcaatcaat aatcatctat gctctttttg cctttcttgg attaattgag
     1741 ggcaaattgt accttttgag gatatgatag tccagcataa aaacagctgt caagcacaaa
     1801 ccgctgtaaa ccgacaaaaa aacccgtcaa ttagcggatg cattcctaac agtgcttgac
     1861 aataagactg ccggcggcta ctatggatgg aacgtattca tatcttattt tttgggagta
     1921 tgttatggac tgcccaatct gcaagacagc aatgatagtt ctcgaattgg aggaggtgga
     1981 agttgattac tgcccccaat gtgtcggcat ctggctggat gcaggcgaac tggaactgct
     2041 gctggatgat gcacacaagg ccaaggagct gctggagtca tttaaggcgg ccaaatcaga
     2101 cgaaaaaaag cgaaaatgcc cgatatgccg caagacgatg gaaaaggtac tcgttggcca
     2161 acacgacagt aaacaggaat tgatagaccg ctgccctcgt cagcacgggc tgtggtttga
     2221 ccgtggggaa ttgcaagctg ttttgacgat ggggcggttt gacgatgccg gccgcatcca
     2281 acgcctgctg ggtgatttat tctgcccaga ggccaaaaat ccgcaaaagt tgtcacaata
     2341 actggctttg ggcgtttata tcttaaaaaa accaattaag aagaagaagc ggtggataac
     2401 gcagcaaacg gatttgttct gatgccggtg ggcgatatcc cgctgtatat ttgtttcggc
     2461 aagcggcaat tcgccaggcg gggcgttttg gggaatgcga aggaattttg gttgacgccg
     2521 gatttttttg tcggcactgg caggacagta ctgggacgac atctggatag agggtaaaca
     2581 aagctgcttt atgcagcgtt agtagttaag gccggttgtg gatcatagcc aagctggtga
     2641 gtgaggcggc atagcaggag aaaaaaagcg gtctcatgcg ttgtccgggg tgtaaagggt
     2701 tgctgcgggc cgaaaggcac gactatatcg agtttcatat ttgctcgcat tgtcgcggca
     2761 tctgggttgc cggcgagcag tttcatgcct tggcggtacg ggtggcagcc gaaggacaag
     2821 tgccgtccag cgccaagctg acattcgagc cgcgcaaagc cctgaagccg gcagaagata
     2881 atcctgcacg gctttgtccc gtctgcgaca aaccgatgca ggagtttagt tatgcctatg
     2941 actccaatat ttttctggac cgctgccggc agtgccgggg aatctggctg gacccgaatg
     3001 aaattatcga cattgccgct catattcagt ataatccaga tgtggatgca atcggcaggc
     3061 tgctgataaa ggatgaaaat gctcaggatg aaacccaatg gctgtatatg ctggccgcag
     3121 cggcattggc ggttttgcgg gcagtgcttt ttaagcggtg ataaaaacaa caactgcaaa
     3181 ccagcagggg aatatgaaat aataggtgca tttggcacag aagatgaaca aatacggccc
     3241 agaaacatca tatagagcag gaaaggagta atacaatgat attgagtgcg ggattgatcg
     3301 ggatgctggt gattttggga gtagtggtgc tgctggtgct gtttgtcatc ggcgtttaca
     3361 acagtctggt gcggctgcgc aatcaggtgg ataatgcgtg gtcgcagatc gatgtgcagc
     3421 tgaagcgccg tcatgacctg atccccaatc tggtcgaaac ggccaaaggg tatatgcagc
     3481 atgagcgcgg cacattcgag gcgattaccg aagcccgcag ccgggcgatg ggcgccaaat
     3541 ccgtagccga ggccagcaag gccgaaggcg ccctgaccga ggcactgagc aaatttatgc
     3601 tggtagtgga aaattatccg gatctgaagg ccaatcagaa ctttctgtcg ctgcaggaaa
     3661 cgctgaccag caccgaaaac aagattgcct ttgcccgtca gggatacaat gatcaggtgc
     3721 tgttctacaa caacaagatt cagatgttcc cctcgaacat catcgccggc atgttcaatt
     3781 tcaccaagcg ggattacttt gaaattgaaa atgaagccga acgcagtgtc ccgaaggtca
     3841 gttttagctg atgattggag agtctgaaga gaacagcaca acagcggccg ggattgttaa
     3901 aacaggaacc gaaaatgcag aatatctttc agcatcagga atgcgcaatt aaaaatgcgg
     3961 gaattaatac gcatcaatag acgcaattcg attctgctgc aggcgctggt tatgaatgtt
     4021 atatcagggc tctgcggcag aataggaata agcgagtgcc ttgcctctgg gatggcccaa
     4081 gaggcacccc gtgccgcccg cagtgtgcac ctttggtatc cggcgccgga gggcatggtt
     4141 tattacaatg aactgaccgt ggaacaatcc gcaccgggca gctatttttg cgtatgcggc
     4201 tttaagaatg gttattttgg cattcaggag atcagcccgg aacggaaggt tgtcattttt
     4261 tctgtctggg atccgggcaa tcaggataat ccggacatgg taccggaaga tcagcgcgtc
     4321 aaggtgcttt atgccggcca gggaatccga atcaaacggt tcggcaatga gggcaccggc
     4381 gggcaaagca tgtttgacta cgactggaag atcggtcagc gatatcgctg tatggtcaag
     4441 gcagctgttc aggatcagcg gaccgcctat gcagcgtttt tctatctgcc cgagcagcaa
     4501 acatggaagc atctggcaac atttgaaacg atcaccggcg gtaaatccct gcagggatat
     4561 tactcgttta tagaagattt tcgaaggaac ggccaaagtg cacaacagat tcgccgcgct
     4621 cgctacggaa acggctggat taagacagtc gatggtcagt ggatagcttt gagcaaagcc
     4681 gtttttactg ccgacaacaa tccgtccata catattgatg cgggtaggat tgaaggcggt
     4741 ttcttcctgc aaaacggggg agagactaaa aacagcaccc ccctgagttc atcgctgacc
     4801 tgttcaaccg attcatttga acagcccgaa gatccccagt ggaaactaat ctggaatgac
     4861 gagtttaacg gacagggact ggtagacgag gaaaaatgga actacgaaga aggctttatc
     4921 cgcaacaacg aaagccaata ttacacacgc cggcggccag agaatgccag acaggaaaat
     4981 ggaacgctgg taatcgaatc ccggaaggaa gcgtttaagg agggcaaata tacttcagcc
     5041 agtattcata cctccggcaa acagtcgttt ctttacgggc ggtttgaggt ccgcgccaaa
     5101 ctgccgaccg gcaacggaac ctggccggcg atctggatgc tgggtgattc catccatcaa
     5161 gtcggctggc ctgcctgcgg cgagatcgac atcatggaaa atgtcggata cgatccggaa
     5221 actattcatg ccaatattca taccaaggcc tataaccacg tcttgaaaac aggtaagggc
     5281 tcaaaaatcc gtgccgacaa gccgtgggaa cagttccatc tgtacgccgt ggagtggtac
     5341 gaggatcatc tggatttctt tatggacaac cataagtatt tcaccttcca aaacgaagga
     5401 accggccccg acgtttggcc ttacgatagg gcacactacc taatcctgaa tacagcgata
     5461 ggaggagcgt ggggaggaca gcagggaatt gacgacagta ttttccccca gaagtttctg
     5521 attgattatg tacgtgttta tcaacagcaa aattaatttt tttgattgtt atctgctatg
     5581 tgggaactga tacgtattaa taaacgcaat tcgattctgc tgctggcagc aatgggagca
     5641 gtcctgctgc tgctggggta tattctggga gttttgttcg ggggcaatcc gcaggccggc
     5701 tggatgggaa tgacggcagc ctgctgcatc tggctggtgc tgatgctgat cagcttatcc
     5761 agcggcgacc agattctgct ggcttccagc cgggctgttc ctgtaacagc ggatgtgcac
     5821 cctatgctgt ttaatgtggt ggaagagatg accatagcag ccaacctgcc gaagatgccc
     5881 aagatctata tcattaacga tccggcccca aatgcctttg ccaccggccg cagcccgcaa
     5941 agcgcctctg tggcggtaac tgccgggctg ctggcccggc taaaccggga tgagctgcag
     6001 ggtnnnnnnn nnngccatat tgtgcatcgg gatattttgt ttgtgacact ggctggggtt
     6061 atgctgggca gcattgtgct tatcagcgaa attttcatgc ggagtctgtt ctatggatcg
     6121 ctgggatcgc agcggcggta ttcgtcacgc tcctccaatc agggcggcgg acagctgatc
     6181 atgtttgtcg tggcgatttt gtttgcgatt ttagccccga ttttcgccca gctgctgtat
     6241 tttgccctgt cccggaggcg ggagtatctg gccgatgcag gggctgtgcg gctgacgcgc
     6301 tatccggagg ggctggccag cgcgttggag aaaatagccg gctgcccgat taatatggag
     6361 tcagccaaca aggtaactgc tccgatgtat attgccaatc cgttccgcgg cagaaaggcg
     6421 gtccatctgt acagcacgca tccgccgata gaagagagga ttcggattct gcggaatatg
     6481 agtcacggtg ccggatatat cgattatgaa aaatcctatg aggcgataac caagcgctcc
     6541 cctgttgtac cgatgtcggc tttgaaagaa gaagcggtag caattcggca gggcgcggct
     6601 gaaccggccg caggcccgac agctcggcag caggcccgcc ggcttggcga cctgatgcgc
     6661 acggtcagcg catttgcatt tctaacctgt ccgggctgcg gattgaaaat gaaaatcccc
     6721 ccggagtata aaggcagccg cgttgaatgt ccccgctgcc ggcatatcgc agtgctcaag
     6781 cagcagcaaa ccaatgcata ggattttgtt gggggtctgc ccgggaaaga aagaacgcgc
     6841 ggcagctacc gcattaccca gcggctttca atttctttca atgcagccgt gtcccgaagt
     6901 ctttcgtcgc cgccaatgct gtatttcaat tggagagttt tctgcataaa gattttgacg
     6961 ggattgccct gcgggtcctt ctggacagga tgaagcacca cagcggtttc attgcttaag
     7021 ccgccgatga ataagctgat ttggcgggcc ttggggtcaa aatccggcca gatgatggca
     7081 aaatcgcggg tattgtcttc gcctgcaaat atgcggtgct ctacaaaatc cagcgattca
     7141 aggaacggat aactgccctg atgttttctg gcaataacat caaaaaccgc cttgggaacc
     7201 tttttgtcgg catcaattat ttggaaggta tccgtaacaa gctgacaaac agggtaaaaa
     7261 tcaaccgtat caaacgaact gttgttggta actgttaaga tgatatacca gaatcgctga
     7321 ggggcctctt tgcccgcaag cttgaccaga agctgctcgg gctgggaata cttgacctgc
     7381 aatgtccact gtccaatcgg ctgaacgatg gccggggcag gataagaaaa aacaggtgat
     7441 gaggcaccca gcagcaaaac cgcagctgat aataaaataa ttccagattt cattaaaata
     7501 tccccaatat tattattgcc agatatatta agtatggtac attttactgc tgaatcggcc
     7561 gcaggtaaag aaaaacttca acgagaagtc gggttttacc caaaatcatc gcatagagga
     7621 gataattagt cttatataac agattgcccg gggtaaacct atgctgaaaa aatatacaat
     7681 tgaaaacggc aagctcattg aaaacggcag ggacgatgcg caggtacatg tttatatcgc
     7741 accggacgaa gcggaaaaaa ggtatctcat cgaccagcta aagattgatg agcataccct
     7801 caattcatcg ctggaccctg atgagctttc ccgactggag tttgagccgg agcatattgc
     7861 catcattttc aaaaggcccc gccattatca ggcatcggat gatttcttat tcaaagtgct
     7921 gtcggctggt gtatttgtct tcaaggaccg gctgattatt gtaatggggg aagaagcccc
     7981 gctgtttgag ggcaagccat ttatcaagat tcaaacaatc caagatgtga ttctcaagct
     8041 tttgtaccgc tccatatccc attttgtcga acacctcaaa ggcatcgatg cgatggctaa
     8101 cgaaatcgaa aagcaggtca atgtgtcgat ggagaataag tatctgatta atatgtttac
     8161 cctcgaaaaa tcgctggtgt attacctcaa cggcattatt tccaacggag ttctcattga
     8221 gaagctgaaa aataatgctg taaggatcgg cttctcgcag gaaaatatag agtttctgga
     8281 tgatatactg atagaaaaca accaatgcaa agggcaggcg gatatttatt cgcaggtgct
     8341 ttcatctctg atggatgcgc gggcatcgat agtaagcaat aatctgaaca ttcgaatgaa
     8401 gcagcttact attatcacta tagcgattat gctcccgaca tttgttgtca gcttgttctc
     8461 gatgaatgtt cggattcctc tgtcgcagta ccacaatgct ttctggatca tttcggctat
     8521 ggccgcaatg accacaacta tcgttgcggt actatggtgg aagcgtcggt ggtagtccta
     8581 taattgtatg aaaaatgaaa gataagggcc tatgcctttt agagggggta taattcctgt
     8641 ccgaaccggg gattttgcag acggcaggaa acatcatggt tgacattgac aataaagtta
     8701 ttctacttta taatgcatta ttttaagcct gtcttttgaa atatagcagg gcaattagtg
     8761 cccccggaac tgttagagac cgaaaaagac gcgtaagcag gcccaaattg tcttaaatag
     8821 attaaaagat atatttcaaa agaggcaatc tgtggaataa taagccgatt tcggctgggt
     8881 tgaggactaa tacttgttat ctatttctta gcacagcagg agatgtttga tgaaaaatca
     8941 gccgctggat acccatgtcc agaccgagac aaacaagacg cctgaaatag agaaattgtt
     9001 ccgggcagtc attaaggccg atgccagcga cctgcacctg aaagtcaata tgccgcctaa
     9061 aatccgggtt cacagcaagc tcaagagcac cacaggcgat cccctgaccg aagaaaagat
     9121 tgagcggatg gtttttgaga ttatgagcga agaccagaag cagtttttct taaaaaacgg
     9181 agcgctggac tttgcctatc aggtcggcga tatggaccgc ttccgcatca atgtctttcg
     9241 ccagcgcagc tatatttctc tggctgcccg acggatcagc ggacgcatcc cgccgtttga
     9301 atcgcttcat ctgccgccga ttgtggaaaa aatcgccgaa agccacgacg gactgatttt
     9361 ggtagtcggt ccaaccggct gcggcaaaag tacaaccatt gcttcgatga ttgaccatat
     9421 caaccggctt tatgcctgtc atattgttac gattgaagac ccgatagaat ttcttcacgt
     9481 ggacaagaag gccattgttt cgcagcgtga gatcggcatt gatgtgccgt catacgaaga
     9541 ggccctgcgg agtctgatgc gtcaggaccc ggatgtggtg ctggtcggcg agatgcgcga
     9601 ccaggaaacc ctgacagccg ccatgcgggc tgccgaaaca gggcatctgg ttttcggcac
     9661 actgcattcc aacaattgct cgcagacgat tcagcgcata ttggacctgt tcccgcagga
     9721 agagcgcgat ctgatgcgtc agacctttgc cctgaccatt cgggccatta ttgcccagca
     9781 gctcctgccg gggattcgtc cggatgtgcc gcgggtaccg gcagtagaga ttatgattaa
     9841 caccccgatt attcgaaaac ttatcagcga ccagcgcgag gccgatctgc cgacggccat
     9901 tcgggccggc gagggcgaag gcatgcagga tttcacggaa agtctgcgcc ggctggtgga
     9961 agaagagttt attgatttga aggtcgctct gcaatatgcg ccgaatgtag aagaactcaa
    10021 gatggccctg aagggcattc gctcgacggc cagcggtatt ttataaatcg ccaaccctat
    10081 attgccgatg cgggagactt atcaatgccg atggatgtga ttgcttctgt ggattacggc
    10141 ggctatgtat cgctgtggaa gctgctgttt tttatgctgg cgtttgttct ttgggcgccg
    10201 atggtgaatt gggtctttac ggattcacag gccgtccgaa ccaatacgct ggtctggacg
    10261 ctgaccatcg cactggccgg aatcggggca ttgtttgtat ggctgctgat accggttttt
    10321 atcatcggtt tgcttttgta tctgctggtt tttgccggcg cttccatggc ctatgttatc
    10381 caccgcaatt cgcgcgtggc cgagtttgaa aaggttctca ccagcgagca tattcgggga
    10441 ctgcttgtca atccggccaa aaaaatggaa aaagccagcc gggggattac cttcatcacg
    10501 gccaacggca acgaggtccc tttgcccgag cccaaaagcc gggaggcgga aggatttatt
    10561 ctggcctgtg aagtgatcga tgatgcggtc tatcatcggg ccgatgtggt ccgctttgtc
    10621 ccccagaaag aggattatgc cctgctgtat gaaattgacg gcgttaccac ccagcagaat
    10681 ccgcgaaccc gggaagacat agaccatttt gtctattatg tcaaagaact ggccggcttg
    10741 gaggtggagg aaaagcgcaa gccccagaaa ggccgcttta cggctgtgct tccggacaat
    10801 aagcggacca gctgggaagt cagcacgtcc ggctcgacag ccggcgagca aatccggctg
    10861 gaacggatat ccgaattggt ttcccgaaaa attgccgatt tgggcttgaa tgacaatcag
    10921 tttgaatcga taaacagcct gcgcgacatc aagagcggtc tggtgattat ctccggtccg
    10981 cctaaaagcg gcgttacgac gacattttat actctgctgg gcaaccacga tccgtttatg
    11041 aataatatca atacgctcga aaagaatccc gccgcagagc ttcccaatat aacgcaggtg
    11101 cagttcacat tgggcgattc gggagaaaat acctatgcac ggcggctgca gagtgtgctc
    11161 cgacgcgggc cggacatcat cggcgtagcc gactgcgaag acgaccagac ggccaagctt
    11221 tgcacggctg ccgccaaaga cggcaaaatc gtgtatgtct gcatttccgc taccagcgtc
    11281 agcgatgcga tggataaatg gtgcggctgg gtcggcgaca aagggctgat tgcggacacg
    11341 ctggaagcgg ttatcaacca gcggctggtg cgccggctgt gtatggagtg ccggcaggcc
    11401 tatcagccca atccggccct gttcaagaag tttaacctgc ccccggacga tgcccggatg
    11461 ttctataggc cgggagaaat tgaatacgac aagcacggca agccgattgt gtgcaagaat
    11521 tgtcagggaa ccggattttt cggacgcgtg ggattgtttg aaaccatccg cattaccccg
    11581 gaactgcgcg atgccatccg caaggccaaa acagtgcagg aaattaccac cgccttccgg
    11641 cgggccggaa tgctgtatat gcaggagcag tcgattaaga aggtttccga aagcatcacc
    11701 agcatcaatg aggtcattcg gaatttttca gcaaaatcat aaggcggccg aaacggctct
    11761 gtgcgaggga tcaatatgga actggcaatt attgtaattg gcattttact gacgatggcg
    11821 tatttctatc tcaaatgcac ccccatgtcg tctttatcga ctctgctggc gtcaattttt
    11881 gcattgatta tcgctttcag ctggtatgag aagatcaccg agctgtttat ttcacgcggc
    11941 tacggcgtcg attgggcgca tttcggctgc tttctgctgt cgtttatctt gacctttgct
    12001 ctgctgcggg cgctggcgga tctgctggtc ggggtcaata tcgaccttgg caagcccgtc
    12061 aagctgatta ccgcccttgt ctgcggcttt ttgacaggga ttattctgac cggaaccctg
    12121 ctggtggcat tgggtatgct tccgctgcag gggaaggtgt tttacagccg cttcgatccg
    12181 tccaagccgg tctcgctcaa cagccctaag gtgccgctgc tgaatgcgga tggatttgtg
    12241 gttggtctgt accggctggt cagcgccggc tcgatgagtt caggcaaaag cttcggtgtg
    12301 gtgcatgccg acttcctcaa gcagatacat ctcaaccggc ttcgggttca ggaaggcgtg
    12361 ctgacggtca gctcccgcaa ggcattgata ctgcccagcg gcaaaaatca aaaaccggtg
    12421 cgggaatggg atatacccaa cagaggaaaa ttaaccgtcg tgcgaatggg cattgtggcc
    12481 cgaaatattg ctgacggcgg ggccaacaat tcatccggac agatccagtt tttcccgggt
    12541 cagattcgaa tgatttgcaa gccgtccgga acggatgcaa aaaaaatgct ggccggttcc
    12601 gggaaggcga tatggccagt cggatttatc gagggcggca agcttgtcga aaagcatctt
    12661 gaagaaataa tcacgcccga aacaaagggt ctcaaagacc ggaccatctg gctggatgcg
    12721 gcctttgatg ttccccaagg tcaggaaggt gttcttctgc agtttaagca gtatgcgatg
    12781 gtgcagctgc ccaaggccgt gtccacttcg gatgaggtgg agaaagccct tcttgaaggt
    12841 tctgcggaag ccaacgccga aagcagcccg acagagtaaa cggctcctgt cattgtccaa
    12901 ataaactcct ccgcctgaca gcagactttt ttgaccggct cctgctcagg aaatacaaac
    12961 cagcagacaa atcagtgcat taggcgcata agcagacctc ttccaggtcc acctgcatga
    13021 gatgctccag atacaaatca aggtcttcga cgctgaactg ttcgaggaat tcgggtttgg
    13081 cggatttatt gatttcgagg atgcagtcga taatttcgtg tttgctcatt actcataact
    13141 ccgttaacga atctcttttt cttccttgac ttagtaagca tatcggacaa tgccgcaaaa
    13201 atacttgaat ggcgctaaaa aagattttta agccggccgg tctcctccgg atactacatc
    13261 tatgacggca gaaccgctga cacacacaat attgaaggtt tatttatcgg cacttggagc
    13321 gtccaataaa actctcaaaa gcagaggaaa aatagagccg ctgaatttgg aaatttttca
    13381 tagtgccgga tataataatc agaaagaact gtcggggaaa aattattgcg ggatcaatat
    13441 ggatttacag gacaatgcct ttaacgccgg acggatgcgg catcagccgc agctgaaact
    13501 gtggcgctat gccggcctga tgctgacata ccgctgcacg gcggcctgcc gattctgcta
    13561 ttactattgc agtccgcagg ctggcgggct gatgacagcc gatacggcga tagccgcttg
    13621 gcagggactc atccgcatcg ccggcaaaga tgcccgcgtt cacattaccg ggggcgagcc
    13681 gtttctttgt tttgaccgtt tggcagaaat ctgccggctg gcctgccggc ttggtctgac
    13741 gccgattgat tcgattgaaa ccaacggatt ctgcagcggc agcgataaag aactgtctga
    13801 aaaactgcag tttttgaacg actgcggatt agaacggctc aaaataagct gggacccctt
    13861 tcacgaggaa tatattgata ttgaacaggt taagagaacg gttttcattg cccgccggat
    13921 actcggcccc cagcgtgttc tggtcaggtg ggaaaaatac ttgtcggctc catcgggcat
    13981 tcgacagcaa agccaagccc agcggcaagc tgttttacag cgtgcgttgg cggatgatgt
    14041 ctgccgattt accggccggg cggcggaagc gctggcccat ttggctccgc agcagccctt
    14101 gacgcaattt agcgataaaa attgtcgaaa tgctcttctg tctgccaagg gtgtgcacat
    14161 tgaccccgac ggcaatgtct ttgtcggcca gtgcagcggg atgattctgg gcaatgcggg
    14221 ccggacgccg ctggatgtca tctggaaaaa ctggcagccg gacagagggg atttctgggg
    14281 cgtgctgtat gacaaagggc cgtttggttt tcttgaaaaa gcccaaaagc agggatttca
    14341 ccctcgcagt cagtatgcat cgaagtgcca tctttgtacg gacattcggc gttttttctt
    14401 tgacaatcag tttttttcta cgataattgg ccccagtgat tgttatggca gatagaaaat
    14461 gcggactgga gattcgtctt ggccgccggt gatacaacaa gctacgatac gatagtcggt
    14521 cgcatggtcg ttgagcagcg gctgtgtacg gaagaagaag tgcgccgctg tctggaggaa
    14581 ttaaaggaac gggcatccag ccggccagct acactcgagc agcttcttct cgaaaagcag
    14641 tttttaaccc ccagccaagc cgcccgcctg caaagcatga ttcgcgaaag caaggatgtc
    14701 tccagtcaga ttcccggcta taagattctc ggcaagctcg gttccggtgc gatggccgta
    14761 gtgtacaagg ccaagcagat ttcattggac cgcacggtgg ctataaaagt gctgccgaaa
    14821 aaatttgtcc agaagtccga ctatgtagag cggttttaca aggaaggccg gattgccgcc
    14881 aagatgaacc acaacaacat tgtgcaggcc atcgacgtcg gcgaagtcgg ggggctgtat
    14941 tattttgtaa tggaatatgt cgaaggccgt acgctgtacg acgatttgtc caaaggcaaa
    15001 atcttcagcg aaaaagaagc cattgatatc atccttcagc tggccagcgc tttggggcat
    15061 gcccattcgc tgggactggt tcaccgtgat gtcaagccca agaatattat gatcagcaag
    15121 gacggcgtgg tcaagctggc ggatatgggt ctggcccggg agaccaccga tgtcaaggca
    15181 gccaagcacg aacagggcaa ggccttcggt acgccttatt atattgcacc cgagcagatt
    15241 cgcggactgg tggatattga cggacgagca gatatttatg cactcggggc aaccctgttt
    15301 catatggtaa ccggccgtgt gccctttgag gccagcacgc cttcggaagt gatgcgcaag
    15361 cacctcaagg agccgctgac cccgccggac catatcaata cctcgttgac agccggtatt
    15421 tccgaggtca tcgaggtgat gatggccaag gagcgggagg aacggtaccg caatatggag
    15481 gaagtgatcg ccgaccttca ggcggtgcgc gacgggaaac ccccgctgct ggcccgccaa
    15541 aagtttgatg tggaagccct tgagcagctg gaggagggaa tggccataga aaccaccgcc
    15601 ggcaccgagc gggtgtacac cgaggaaatg atagccaaat atcgcatcgg gcttctggcg
    15661 cttgggattc tgtcagctat cctgctgctg gtgattttgt ttctggtgat gcgatagtcc
    15721 gcatcaggcg gcatcggcgg actgccatac gattatgtca agacgaccaa ccgatacgcg
    15781 agaacggatt ctcgatacgg caacgaatct tttcagctct cacggattcg cctcgacttc
    15841 gattgacgat atcttaaaag cggtcggcat caccaagggg gcattctacc actatttcaa
    15901 cagcaaggaa cacctctgcc aagtaattct ggatcaggca gtttgtgtat ttcatcagtt
    15961 agctgaatcc cttctggaaa gccccgattc ggcttggccg ctgcggcagt ggattgagat
    16021 gctggcccga aaacagcagt ccggccaatg gcttttcgac cgtcttctgt accgtttaac
    16081 cgtcgagagc tctcaattga gtcctgaagc gcaaaaccag ctgcgaatgt tctggcagtg
    16141 gtgccaggtg ttctatgaaa gtcttatccg aaagaccatc ggggaaaaag gcgattggcc
    16201 caagccgtcc tcgtctgccc gactgcttgc ggcggctctc tttggagccg tatggctgga
    16261 ccgctgcgcg ggctctgatg aggaacaagg agccatccgc caagcactgc tggagctgat
    16321 tgcagcagcc caggcagatc cggcaaaaac agccgaatga acaacacggc ataaacaagc
    16381 cggtgctgat gactggagct ggcgggaatc gaacccgcat ctccgcgatg cgaccgcggc
    16441 gcactcccat tatgcgacag cccctctacg gctgtctatc atatcccaaa ggacagaaag
    16501 cgtcaagaag caaaaacgcc ggaaatcacc aaggggtggt tttaggcttt tactgcacgt
    16561 ataggaccga attgtaatcc ccgaaaggat tcatttcggt ttggccgtta tagggatcct
    16621 gctgccattg gccgtccacc accagccgat agcggtatac ccctctgtcc agcggcaggg
    16681 agagaaccca tttgccggta tcgccgacct tctggaaaga tgtctgctgg ggatcccagc
    16741 cgttgaagtc gccggcaatc tggacggttt gcgcacgcgg atacagggtg ctgaagacaa
    16801 cctgcccgtc aatctgccgc acgccgtaaa agtcggccag cttctcattg aacgacaaag
    16861 ccggctgggc tttttcttcc tgcttggccg gtccggtgct ttccagcagc tcttgggcac
    16921 tggcgctgat agtgctcaga cgggcctgca gcgaattgac cagattggca cggtgcattt
    16981 gggtatcggc gccgatcagc tcatcggcta aagccaagaa atccttatag cccttgcagg
    17041 cagggtcata ttcactgatg ggctggccca agctggcggc ctctttgagt tttgtgttga
    17101 aattaaccac ggttttgaac atccgctccg agaagtgctt tttcaattct gccagaattt
    17161 cccggcccat tttggtgcgg atgtcataca tactggccag caccatcaca ttaattttct
    17221 gttcgcactg ctgacagaga atattgagtg tttccagctg gcggcttaga ccatgcagcg
    17281 aaaaatagcc catttctacc ggtacaataa catccgtggc ggctcgcagg gcattgaacg
    17341 tcagcagacc gactgacggc gggcagtcaa tcactacata atcatactct tcccgaacgg
    17401 catccagtac tttgcgcagg cagttttccc tttccttgac gccgttcatc tgctgctcaa
    17461 aggttgccaa atcgatgctc gacggcgcca gctcgaaccg gtcgcaaatc tgccagagta
    17521 tctccttcag ccgcatcggt tcgccgcgac ccatcccgat gagaacatca tagatgcttt
    17581 gctcaatttg ttcttccggc actgccagac cgacagcaca gtgaccttgc gggtccatat
    17641 ccatcagtaa tgtccgataa cctcgtccgg ccaaggcagc ggacagatta atcgaaacgg
    17701 tggttttgcc gcaaccccct ttttggttga taactgctat ggttctcata gtatttctcc
    17761 ctataccgcc tataatctta gaaaaatccc tttttctgta taacgataat aaatctataa
    17821 cagctctttt ttcagtatcg gaaaaatgaa ttgaaattct ttagagtttt atcggacaaa
    17881 tcctaaaaaa tcccaaattt cagtagtatt atggggcatt tttagtgtca gaggaacccg
    17941 cagaaacaga acaaaaaaag tctggttatc ggtttaaaat caaagttgaa gcgttttctg
    18001 aatgtctgcc gataataatt atagcagtaa ttgacagccg gaggaatttt gccggctcga
    18061 ttttcatgga gctatagaaa tgactgaaga gcagattgtc aaatggatgc gtgaaagagt
    18121 tcaaaaaaac ggctttacgg atgcaacatc actggctgaa agctttttaa aagaatacaa
    18181 aataagcgat gctttagacc ctatattttc acggtctttg gatgccggct tcaaggtagc
    18241 tcaggaaatc cgcgaccgcc ggcttgcatt gcagcccaat tgagcaaaaa ggagcctcca
    18301 acagcgtttg cttgggcagt acacatttca tccgcatatt tttgctgccg tttaattcgg
    18361 aatcttctgt tgcccaaaca ggcatagcac cgttgcagca ctgccggatg tatttacaaa
    18421 cttctttgag tacactatgc tggaagagta tttctacggt ttgatgcggc tgatcagagt
    18481 gtcgatgcgg cggcggaact gtgcatcggt tttgtacatc tgttcgattt tggcacaggc
    18541 atgcataact gtggcgtggt cgcgcccgcc taaatgcccg ccgatttcct ccaaactgtg
    18601 gcgggtcagg cagcgtgcca gatacataca cacctgccgg ggcagggcaa tgctttgact
    18661 gcgttttttg ccttgaatat cggcaattcg cacatcaaac tgctcgctga cggcccggac
    18721 gatttccgat atcgttatgt tttttgcagc atccgcacct tgaacgccca gcgcctgccg
    18781 cgccagctca acagtaatgg gtactccggc tgtcttggac atggcataaa tcattgttag
    18841 cgcgccttcc agctcgcgaa tattgttttt gatgtgttcg gctaccagtt cggctacacc
    18901 gtcaggcagc tccagtcccc gcaggctggc cttttttttg acaatcgcaa tgcgggtttc
    18961 ataactgggc ggatccagac gggcgaccag cccccattta aaccggctga taagccgctc
    19021 ttccagcgcc gccaaatcct gcggggcgct gtccgaagtc agcacaatct gccgccggtt
    19081 gttgtacagg gcattgaagg tatggaaaaa ctcttcctga ctctgttccc gttcgcgaag
    19141 aaactgcaca tcgtcgatta tcagcacatc aactgtgcgg tggagactct gaaactccga
    19201 cagtgttcct ttttcgatag cgctgataaa gccgttgaca aattcctcgc agcttaaaaa
    19261 ccgaatggat atgtccgcct gcttcttgcg ggtatcgtgg cagacggcgt ggagcagatg
    19321 tgtcttgccc agtcccgcgc tgccgtaaag aaacagcgga ttgtaaatca caccgggatt
    19381 ctgactgacc gccacacagc ttgcatgggc cagccggtta cacggcccga cgacaaagtt
    19441 ttcgaaggta tagtccggat gcagccgaag acggccggcg gcttcagccg gcagagtggg
    19501 tttcttgtgg gggctgccgt tgtacagaaa ctgcaccgat accaaatgcc ctgtaaccgc
    19561 ctgtgcggcc tgcgtgaaag taccctgaca gcggtcctgc agaaattcgg cacacgactg
    19621 atcggggcac tgaatctgca ccaagccatg gtcgaaagcc gcaatatgca gattgtcaaa
    19681 ccacgtgcgg gtattgaccg gatcaatcgc tttgacccgt tcaagaatcc cgtctaatat
    19741 ctcggtactg ttctgtgcag ccaaatgaac tccttatact gccaagctca aaacacggct
    19801 ttttaatccg cggtgtttga gatggcatta aaaatatcag taaaaaagta tccacacttc
    19861 gcatttctgc ggtcaaacag gcagtaaact ctacaactga cacctgacct tgtcaaaggt
    19921 ttttatcacc gaggatagac aggcaaccgg ctttgccgca gctaagtgcc gacagttcaa
    19981 tgacttagtg ccaacaatcc gatacagtgt gattcgaatg cacatatctg tcgggcatct
    20041 gcttacggcg tcagccagtg cagaccgaaa gcggtaaaat cgcccagatt gagcagtcca
    20101 tcgccgctgc gtcggggaat gatgtcataa tagggcgtat cggtcagcca gttgtcggcc
    20161 aaaatgatca ggtcttcagt atcaatatac cggtcgcggg taaaatcggc ctcaaacggc
    20221 cgcacctccg gcagcacccg catttcagta gtaaagtggt agtcgtaatc gtccagctgc
    20281 cccaaaaacg atcctgaaaa atcaaacacc agctccacaa aataggaccg ccacccggtc
    20341 ggcggcacac ccggatctgc gatgtacagc cccgacgaaa cagccgtcag cggcgtcggt
    20401 atccaggcat cgccgatcgt gacccgccga aaatcccgtg cagcaggatt gtctgcctgc
    20461 cagagtgtta cagacagcgg cgtagccgat gtctggatag ccatactccc gtcgggctga
    20521 gtggtccagg taaatgacgg acgcgcttgc cccctcaaaa aagcgtcata gtaaggcacc
    20581 atattagcca cgacctgttc ataagccccg tcgaggtagt gattggtgtt gggcatagtg
    20641 cggacgaacg tttctccgac aaggtcatcg atatagaact ggatggagtc gtggacgaag
    20701 aaatcatcgc cggcggcagt cacaataaac ttgggaatgc ccagccggct gcggtattca
    20761 tagggatcaa caatgctcat cagctcgctg gtgcggggat gctcaaacca gttgaaaatg
    20821 cccatttcct cgtagggatg cagcgcctca gcccagaagc cgtagcatga ccagtggtgg
    20881 gcaaaactgc ggcgcatgtt cagcaggtcg ctgacaatcg gagcgatggc cgctacccgg
    20941 ctgtcgacgg cggcggtaag ccaagccgtc cagccccgtt tggagccgcc ggtcagcaca
    21001 aagtttttaa ctgccggccg aaacgcctgc accgcattca tacaggcgac aacagatttg
    21061 accatcggca gctgcaccgg ccaatagagg tcgcctccgc tgaggaattt ttcccacgaa
    21121 taggcgataa tctcatcctc gctgcggccg aagccctcat cggcaaacac cagcggctga
    21181 ttgggcacgc cgcgcaaatc aacaatcact gagcgggttg caagggccaa atcacggaac
    21241 tgccggttga ctggatcaga cgggtccagc gtcggtgccg ggtcagtaat gctgcccccg
    21301 ttgattaaaa ccaatgccgt gtttttaaca ctgcccagca gcaatccccc cggcacaatg
    21361 accgtgaccc agtgcgtcca aaccgaggga gtaacttcgg agctgctgcg ccatgtctga
    21421 gaagtcaagc gcagattgac ggctgttgtg ctgactgtcc attggctgcc gtcaaaccac
    21481 gatgagccaa cctgtatcca gctgtacgac gaatccggac tggcgacata atcatccagc
    21541 gctgttccaa aggaaagaga ctgccagacc agacagaaaa aaatgatgct gctgtgtttt
    21601 ggacacttca tcggacaccc tcgctctcat tctttttcca gccggcttgt aaagccagcc
    21661 ggatggttcg attttactgc aaatcgaccg gatttgtcaa ttaaaaagcc gttttaaccg
    21721 ttgctgcact tcgaagatta gggatttttt tcgaagttca aggctgccga gttgatgcag
    21781 tagcgcagac ctgtcggggc agggccgtca ttgaagacat gccccaaatg cgacccgcag
    21841 cgggaacagg taatttccgt gcgaatcatc ccatggctgg tatcgcggct ttcggcaacg
    21901 gcatctggat ttgtgggtgt ccaaaatgcc ggccagccgc agccggagtt atattttgct
    21961 tcagacccga ataagggatt gccgcaggcg gcgcatttat atattccttt ttcgaaatgc
    22021 aggtcatatt taccgctgaa cggccgctcg gttcctttct gccgtaaaac ataatactgc
    22081 tcaggcgtca agattttctt ccattgctgg tctgttttgg tgaaaaaggg ggtttcattg
    22141 gcatccattg gtatctcctt ttgtatggcg gcggaagtcg gctgaatctc tcgggaaggt
    22201 tctgccggac agcccttcat aaaagccgct aatgcagtca gcaatgcaat atagacaatc
    22261 tgcatataaa atagattcat gctttaccgg tttaaccaca ccgctcgctg cggcagagcc
    22321 atgcagaagt aatccagttg ttttaaatcg ccggagttga cgatatggct gttttcgact
    22381 attgtaccaa tagcttttaa gaaacccaat gagaaaatcc tgccctgctg tttggctgct
    22441 gcaatttaac aggagaaaaa atgaccagaa agggtatttt tggcaaagca tctctggtcg
    22501 ccgtgctggc tgcaattctg ctggccggct gccagactgc gggaaagccc cgcatgcgga
    22561 tcggctcttt ttttggcgat gcggcgggtc ttacatttgt ggacccgaat catcttggac
    22621 cgcacagccg caggcataat ttccacgaaa aaagcggatt gctttacaca tgcggagccg
    22681 gctttatcga tttagggcac ctgcgtgagg ccgccgaccg cacggaatac ttagcccgca
    22741 tcacctatca gaatctcctg cagcaggacc agagtttttc ctaccgcctc attgaaccgg
    22801 cgcggtatga ggtgaaagtt atttatccgc ccggctgggc atcgctcagt cagcttgaaa
    22861 aagaacgcat cgcttcggag gcagctatta tcttcggcca gtacggcgcc cataccagcc
    22921 taatttggca tgaaatcctg acttggtacg gttttgcctc gtcgggcatt ttctccgaaa
    22981 acatatcctc attttcgtgg gaagacccgt attcggatgt gctgggtacg catctggccg
    23041 ttgaagcaat gcgcaacccg gcgccctatg aagaagcgat gacaagactg attagtcaaa
    23101 agctggcgca gctggatgtt cagccgattt cggtagcaca ggaagcagcg cggaaagtag
    23161 ccggccgctg gtacaccggc gggtattatt tctttgtgga gatgaagcgg cgcaattttg
    23221 atgttggttt tgatgacgac ctgctgaccc cttggctggt gccggagatg tgccccgacg
    23281 ccgtaccgca gccctgtccg cgccccactt tggaaccgct ggcggcttgg ggattttgct
    23341 ttgaattagc actgtatccg gcggaatttg agaagaccaa gatattcaaa gcgctgggcc
    23401 ggaaaaaagc ggtaatttat ccgcttgagg attttcctct gcttatcgaa catatacggg
    23461 ctgaggctca aaaaacctat ggcccgcaga ttgactgccc tgttttttga ttctggacaa
    23521 ccgtttgaca gcaaggatac aacaatgacg cagaagtatc gactggacaa gttaacagga
    23581 ttgacattgc tgctgcaaaa ggcagcagtc tgcttgggca tagcagccct tctgacggcc
    23641 ggttctggct gcggcttgac cggcttgacc gggccccgtg cccggctggg ttatgtcccg
    23701 gcggctactg tgggggtggt gtttgccgac ccggacaatc tgggctcgca tgccgcggtt
    23761 tacagcccgt ttgaaaacag cggcttggtt tatacctgcg gggcaggggt tttggatatc
    23821 gaccacatcc gcggcagcgc cgacctgacc cggtatttta tcggccgggt tcgggaagcc
    23881 ctgaccaagg gccaacagca gttttcctac aaagtctcgg gggagacatc gcggcactat
    23941 attacaattg cttacccgcc ggactgggaa acgcaccctg acaaacagcg gataatcgaa
    24001 gaaatcgcct ttgaagccgg cccctatctg gcctttgatg cgaccatttg gcatgaaatc
    24061 ctgacttggt tcggcgtgca ttttatgggc tttgagccgg agttcaactc ggctttttcg
    24121 tacgaggatc tttactccaa cctgctgggc tgccggctgg ctgtcgatgc gctgcaggat
    24181 gagcaaaacg attatgaccg ggcaatgacg cagcggctga atgctgtcct tcaggaactg
    24241 ggcgtgcagc cccgccagat tgccgttgaa gcggtcaaaa gcgtagaagg ccgctggtac
    24301 accggcggtc tgattcccga tatgaaaatg cggaattttg atatcgggct ggacggtttt
    24361 ataacgcctg tgctgattga gaatgttaaa aactgcccgg atgcccgtgc agttccgctg
    24421 ccggcgccgg cgctggaacg gcttcagcaa tatggttttt ccctgaccca cgaaatcaag
    24481 cccaatgtct tcgagcaggg ggctattttc aaagcggccg gctccaaacg gattgtcccg
    24541 gatgagcact atccggtact gattgagttt atgaagcagg atgctgcccg gcgcggctat
    24601 cagtatagcg actaaacagc tgtattgggg caagccggat gcagtgtgta atcaaaacag
    24661 ctgaaatgga aaggaccccc aatgcgattg tatctgattt acgggcttgt cctgtacagc
    24721 caacttgccg cagggcagac ctattatgcc gacagccggc agggcaatga tgccgccgac
    24781 ggcaccagcc cgcagacggc atggaagacg ctggaaaaaa tgaacagcat tgtctttgag
    24841 ccgggcgaca aactgctgtt ggccgccggc agtgtttttg aaggccagct caagccccaa
    24901 ggtgcaggcg ccctgatcga cggcacgccg gcagctgtct gcatcgatag ctacggcgac
    24961 ggccctaaac cccgcattga cgcgcaggga cggtatcatt cggcactgta tctgtacaat
    25021 acacaatact gggatatcgc caacctcgaa ctgaccaaca ccggttcaga ccgtcaatcc
    25081 ggccgctgcg gcgtctatgt tcacatccgc gatttcggca cagcccattc tattcatctc
    25141 cgccatctct atattcacga tgtcaacggc tcgctgatta aaaatcaggg cgccggccag
    25201 ggtatcctat ggcgcaacga gggccgccgc aggccgtcgt gttttgatga tttgctgata
    25261 gaaaactgcc gtctggtccg ctgcgagcgc aacggcatca taggcgccgg ctactcacgc
    25321 cgcgacagcg gcaaactgtt tacgccgagt cttcgcgtgg ttatccgcgg caatctgctg
    25381 gaagaaatcc ccggagacgg gattgtgccg ctgggctgcg acggcgcact ggtagaacgc
    25441 aatgtaatgc gccgctgcac acggcttctg cccgaaggcg aagcggccgc aggcatctgg
    25501 ccgtgggcct gcgacaacac catcatccag tacaatgaag tcagcggcca caaagccccg
    25561 tgggacgggc agggatttga ctccgactgg gactgcacca acacgctgat ccaatacaat
    25621 tacagccacg acaacgaagg cggttttctc ctaatctgca acaatggcgg ggcttcgccg
    25681 gcgacggccg tcaataccgg caccatcgtc cgctacaaca tcagcgtcaa tgacgggctt
    25741 cgcacgcagc cgacccgtga gggaatgttc agcccgacct ttcacatcag cggaccggtc
    25801 aagaacacca aaatctacaa caatgttatc attgtgccta aaaagcccga tgcccgcatc
    25861 gatgccacgc tgattaagat ggacaactgg ggcggaccgt ggccggaaga tacgttgttt
    25921 gccaataata ttttttatgt ggaaggccaa acccgctgcg actggggcgg cagcattggc
    25981 cattcctttt ccaacaacct ctttttcggc acatttgtca gccggcccaa tgacccgtcc
    26041 ggcatttcag ccgatccgaa atttgcagga ccgctgttcg gcggcgacgg ttttgagggc
    26101 ttgacggcct accggctgaa gcccgattcg ccctgcatcg atgcaggcat tcccgttggc
    26161 ggaaccgccg acttctgggg caatccgctc agcagcagac ccgatatcgg cgtctatgaa
    26221 aagcagcccg ataccccgca gccatccgcc gaaatccagc cgctgccctt tgtggatatc
    26281 cacgatcccc atatcatccg ccacaaggac ttttattaca tctattcgac tggccggcgc
    26341 ggacaggtta tcggcgctat ccgttccaac gacctcaagc gatggtaccg catcgattcg
    26401 ccgctggcgg cagtgcccga ctgggtaacc gaacagattc cccgctgccg ctccctgtgg
    26461 gcctcggatg tgcatctgat taacggacgc tattgcctgt attattcagc atccactttc
    26521 ggcagtcagc gttctatgat agggatgctg tccaacacga cactggaccc tgccgatgcc
    26581 gcttataaat gggttgatga ggggaaaatt atcgaatccg accggggcaa agacttcaat
    26641 gccatcgatg ccgccgttgc cgccgatgag caggggcggg tctggctgtg cttcggctct
    26701 ttctggggcg gaattaaaat gattgagctg gatgccggca caggcaaagc actgggggac
    26761 aatccgaccg tctattcgct ggcccgccgg cagcagggca ccagtgccat cgaagcaccg
    26821 tacattatcc gccgggggcc gtggtattac ctgtttgttt cgttcgatgc ctgctgccgc
    26881 ggcgcccgaa gcacctacaa catccgcgtc ggacgttctg aaaatgttct tggaccgtac
    26941 cgcgatgccg acggcatacc gatgcttgac ggcggcggca cgctggttct ggaaggcggc
    27001 cagcgctaca aaggccccgg ccacaattcc attctgcagg atggcggcac ggattatctg
    27061 gtctatcata cctacgatgc acaaaatcgg gggatgtcga ttctgcagat tcgcccgctg
    27121 gtttgggaca gcgacggctg gccgcacccc gaggcaccgc tggagtgcgt caataggacg
    27181 ccggcttggg atgcaccccg ccggcggatg gctgaatagg cgggaaacta ttgagggcag
    27241 agatcggtcg ctttttttct tctctttgtc agggccccat aatcccggaa ggcggcaagc
    27301 cggctctgcc gccggcacag accggccgac ggtgctgccg acggattgct tccatatgcc
    27361 gaaagcccat ttctcaaaca gcccgacagg gctgcgtcag cttgcatggg ccggtataaa
    27421 ctggccgata atttcgttga gtttggtgcg gtctatgggt ttagacagat acccgtcgca
    27481 gccggcgtcg aggcacttct gctcatcgcc gcacatggca ttggcggtta cggcaataat
    27541 gggtgtttta acgccccggc tgcggagctg ccgggtggca tcatagccgt tcatattggg
    27601 catctgcata tccatcagga tgatgtcata cgagccggat gctcctttct caactgctcc
    27661 aacgccgtcg tcggccagta cgacctccag tcccagcttc tggagaagta tctgcatcag
    27721 cttctggttg gaagggttgt cttcggcaac caatgctttt ccaatataca tagccgttcc
    27781 tttcattgta tccatcatct catttagctc atcgatttgc ttgtatttgt tccagacggc
    27841 ttccggcgtt ttaacacctg ccggaatcgt cagcgtaaaa accgagccga cgccgacttg
    27901 actgaccaca cggaccgaac cgcccatcag ctcggccagc cgcttggtaa tggccagccc
    27961 caagccggta ccgccgtatt tgcgggtggt agcgctgtcg gcctgcgtga acgaatcgaa
    28021 aataatcatc tgcttgtctt tgggaattcc aatgccggtg tcttcgatat caaaacgcac
    28081 ccagatttgg ccctcgtcct caatgcaggc aacgttgaca tacacatggc cggcttcggt
    28141 aaatttgatg gcattgccga tgaggttaat caggcattgc cgcagccgca gcgggtcggt
    28201 tttgattgtt tccggcacca tatcgcactg gagtacttca aaacggattc ccttctgcat
    28261 cgcctgcggc cgcagcattg attctacttc ggtcagcagt tcgccgatgc ggcagtcgca
    28321 gatttcaatg accatccggc cggcgtcaat cttggaatag tccagaatat cgtttatcag
    28381 cgccagcaga cctttgctgc tggtgctgat catctgaaca tagcagcgct gctcttgggt
    28441 gagtgtgtca tcggaaagaa cttcggtaaa gcccataatg gcgttcatcg gcgtccgcag
    28501 ctcatgactc atattggcca gaaattcgct cttggtccgg ttggccgcca cggcctccgc
    28561 cgccagaaca cgggcttgct cctcggcctg acggcattcg gccattttat ggcgcagccg
    28621 cttgttgagc tgctggagct tgaaggtgcg cttgcgga
//
LOCUS       DAOTBO010000025        28188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1379_length_28188_cov_6.438453, whole genome shotgun sequence.
ACCESSION   DAOTBO010000025 DAOTBO010000000
VERSION     DAOTBO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 28188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28188
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1379_length_28188_cov_6.438453"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..1121
                     /locus_tag="PLE88_04885"
     CDS             600..1121
                     /locus_tag="PLE88_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM75837.1"
                     /translation="MDSDRTKTEYFIHLLSSNHYRIYAYIQSVVINEADADDIMQDTS
                     TIMWKKFDDFSAGTDFVAWGISIAKYRILEYRKKKKDCFLSQETLDLLAAQSEKMMPE
                     LERRFQALKDCLGKLPRKDLELLKLRYTHSCSAKMIALRIGTTIHMVYQNMSRIKMLL
                     VNCVQKQLRTGDF"
     gene            1121..2839
                     /locus_tag="PLE88_04890"
     CDS             1121..2839
                     /locus_tag="PLE88_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM75838.1"
                     /translation="MHPDIQDNLRLPRLIEEMHDSVITKEGIADLDTQLKRDPQARQR
                     YLEYAYLHSGLHRFLTSPEAGFAGHIPHDSDLSVYNDALMELAAAETTAPAVEIFHPK
                     QEIHPAVLHGRTERVARTVNKASLFVAVLSLTVMLLFLAYIKFVPVSHSQLVGRLARV
                     ADAQWADASGMIQEGCDLYTGPMKLICGLAEIILDNGTKIIVQAPSEFELESMNQIFL
                     KAGRLVVDVNKQNDRHFVVRTINAAVVDFGTEFGVSFDGRNTHAYVFQGEVEIRNSSD
                     LLRYEKNLTVKEGQGGQVSSSGDISSQTRSQDHFIRSDEFEVRLRAIQGSAYHRWLAY
                     SLQLRKDPSLAAYYTFEKDPANPDLLVNVAAETFGSLNGQLCSASGEAPPAWTQGRWP
                     QKTALAFDRSRRQYAEIAADARLCINGPITLAAWIYCADAKDGGHIVSNRLPPRSFCN
                     YQLGYHSPVEIDWRRNIHLARKLDSNDDSNQIASRQLPELFGWILVAVTHDNETLKFY
                     LNGQLVDSQRWPHQQEFREAPLLIGADYAPGDNSRFNGKIDEIIIAKRVFSGEEIAEM
                     YRAGRP"
     gene            2921..3739
                     /locus_tag="PLE88_04895"
     CDS             2921..3739
                     /locus_tag="PLE88_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75839.1"
                     /translation="MRKQKGFILIKLLVVIAIIAQLLTLIIPGLNMAKRKASMVADLA
                     NVKSTSLAWYMYQGENNGLICSLTPQNAYPWVKNPVDTAGNTPSLTQVTPIVTDEDEK
                     RGISKGVLFEYIKNADVFHCPMDQRKSLYDLSNIFRTYSMPAYLNWSGTLTGTNEIHR
                     FGKIVGPGTKIMLVEEADMRNYNNGAWSFGAPEWTGGGLEWWDPLAVTHGDGSTLGFC
                     DGPAENHKWVDPFTKDRPNKLAKTGTTNYGREAPPAGHTTDIQYIYSGWGYRKK"
     gene            3910..6018
                     /locus_tag="PLE88_04900"
     CDS             3910..6018
                     /locus_tag="PLE88_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75840.1"
                     /translation="MENGKRVLLGVFLVMVGLLQAVTIEWVSDDYGSSFDQPWINLLT
                     SQGYTVTARANQYYRTLDAAKIAALNAADLVIISRKTSSGDYASNSTEVAQWNGLTAP
                     VLCMSGFLARSSRWKWLNADQNEADAIMKVLDPSHPFFWGVPLDANNQVDTIATGVAS
                     VIATTNPGNSTVLATRASDQYIWIAKWDTGQEYYPGSGQYAGGPRVFCGMGQDVSGSA
                     FNVTEAGKSLFLNIVYEMSGAAWNRKPLVNAGPDRIAYIGNAVALAGSAIDPEATVMT
                     ISWTQVSGPAAASIADASAAATTAAFSAAGTYVLKLEASDGELATSDTMTVYVRDPAK
                     DTLIAHWDFDGLPDPNTLIDVSGHGFDGIYHRTTPGEPNVVPGHITGSLKAADFMEIS
                     YWEIPNARAGDPNFNDIQTGATFAVWAKADAQGAVSTPVIFGFGIDAVRFQINANRWN
                     LVQTGLDVFSVRPVFDGFWHHVAGVYDGVNAQFKIYVDGVLDGTTATPSGYLLTTGAF
                     PPQIGNRADAVRMWPGQMDDLRVYNYALSDAQIAALAAEGDLIPYLTAGIDQTVQYTG
                     APVPLDATLIVNDGIPAPMALSWSVTKVPAGVDPEDVIFSDKTAEDPLVTFPTVAGTY
                     VLKLTGSDGVVEVSDEVSLVILIPTCADILAEGLGMAADLSGPAGVPDCYVNMYDLAA
                     MAASWLTCNNPEDTGCIWPY"
     gene            6255..11858
                     /locus_tag="PLE88_04905"
     CDS             6255..11858
                     /locus_tag="PLE88_04905"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOM75841.1"
                     /translation="MKNAKRLLFVVVVALTGLPQAATIVWVSDDYSANFDQPWINLLT
                     SQGYTVLTAADGATTYWRTLDEAKIAVLNAADLVIVSRKTSSGEYASSSTEVAQWNGL
                     TAPVMCMSAYLTRNVRWKWLDGLQSEADAMMQAAVASDEIFRGVPLDGSNQVDVINSG
                     VASTVITAAAGSGTVLATRATDQSVWIARWDAGAEFYPGSGTVAGGPRMFCGLGQDVS
                     GAAYNITEAGRTIFLNAVQSLCFRKGIVGSVAETGGSTEVWEKPAISDTFSVVLNKLP
                     LAAVDVTVQPVSNAADIQIDGSAGPGEPIVLTFTPASWDVPQTVAVRAVPDELEEGTE
                     YAAIEFRIHSTDPDFNDGLIFPVQITVFDSPINDCPTADLNHDCRVDIGDLALLAAAW
                     LGDPASPANLYPDSIIDLKDLSLFSQNWLDQEGPVVISEFMASNGVTISDGDNQSSDW
                     IELYNMTDAAVNLEGWYLTDDAARLTKWRFPAGSVIQPYSYFLVFASGKNDLLYPYID
                     SSGFLHTNFNLNASGGYLALTGPDGQTVVHAYPSYPAQEKDISYGLYRLQNRYFVQPT
                     PLSANSAVYEGLVADTKFSRNRGFYTAPFEVSISCATPGAAIHYTTDGSTPNESSPAY
                     TGPIAISATTCLRAAAFKPGWRPSNTDTQTYLFIQDVISQTAPPGYPAVWNGYPADYE
                     MDPDICTNPAYADLIDDALLSLPTVSLVTDKKNLFDPATGLYLNTTMEGDLWERPVSM
                     EFVDPSGTEKSFQEDCGLRIQGGASRNPNNSPKHSLRLLFKSAYGASKLNYPFFGDDA
                     VDSFDTLVLRADYNNSWIHWDPQQRLRGQYLRDQFARDTQLAMGRMSAHGRFVHLYIN
                     GLYWGLYNATERPEASFAASYYGGEKEEWDAFNAGVVVDGDDAAWKQALSIASAGVSD
                     AAGYAALSEYVDIPNLIDYMLVNFYGGNQDWGAANWYAAGRKTGTERFKFFCWDAERT
                     LEDPVAHNVTGVNAANSPAYFYAQLRANPEFRMQFADQAHRHLFNGGLLTPSACIARW
                     MERAGQINTAVIAESARWGDYRRDVHVRGEAFLYTRNDHWIAEQNRLTGSYFPVRTNT
                     LIQQLRNAGLYPAVDAPTFNINGTYQHGGYVGNPSALTMDVTGTTSYVSTELVSEGAA
                     VRVYMPADNSLGLTWTGQSFSPGADWTDGSTGTGVGYDLSTPYDALIQTDVQSLMYQK
                     QPSVYCRIAFEVSNPSVVSSLKLQIKYDDGFIAYLNGQEVCRSSNISYEIPPTAAAAS
                     HTASAFEEFDITSAVGQLTAGTNILAIHGINSSRTSSDLLILPKLIASVGSTVIPPIW
                     YTTDGTDPRQSGGAVSPSASLYSGAVTLTESCCVKARALTNGTWSALNEAAYAVGPVA
                     ESLRITELMYHPADPNCEYIEFRNIGSQTINVARVQMTRGVTFTFPSLSLAPGEYAVV
                     VQNLAAFAGRYNTAGMTIAGQYAGSLENAGEKIKLVDAAGQVIHLFDYSDDWYKFTDG
                     YGFSLTIKDETNPDLALWDQKAGWRPSAVSGGSPGTGDAGIVPPLGAIVINELLAHSH
                     AAEPDWIELHNTTAHDINIGGWFLSDSDADDLTIRKYEIALDTVIPGNGYLVFYQDQT
                     FGNTTDPGCHIPFALSEGGETVYLHSGLGGVLTGYVEKESFGASESGVAFGRYYKAST
                     DSYNFVAMSVNTPGQGNAYPKVGPIVISEMMYNPPAGGSFDHDDYEYIELRNIEDETI
                     TLEEHDVLLDIDVPWKFTNGIDYTFPLGVSIAPGGSIVVVKNPAAFSERYPDVPAGVI
                     YGPYSGKLDNGGEKLELAMPGDEEAAVRYYICVDRVNYSDGSAPGDLWPVAADGQGGS
                     LHRKNDQLYGNDVANWQAALPSPGN"
     gene            complement(13139..15757)
                     /locus_tag="PLE88_04910"
     CDS             complement(13139..15757)
                     /locus_tag="PLE88_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75842.1"
                     /translation="MPFTVDNTSRMEAYWNKKRIFQLHQADPSAIERFFEHFGPWIYT
                     WLYYQTGADPQIALELTDLTFRRIILNITSFDTAKQSLSQWLMEQAKLALSEGMAGRQ
                     IQPQRPWAWSQLPEHILQALAGLRFQAIPDTTAAYPFVQELVQAALAEMDANDRELMI
                     YRYHHLDMPEHIAEQMGIRIEEVNNRLYRCRHSFRRIFTQLLSAANPAFSESSATGSI
                     ELLDSNLEKLLSSTAIYQTPSPAQRESILSSMLEAARQAAQNQPPKRLMSRTAAIIGI
                     AVIFIILAAAAGILRSLYQKPSAPPAVSAVSNPSAEKEPKKIEQPTASVGEHNDPEKE
                     KLKQVFELGQAGNLDALLEILKSGHFAGQMAAAHFIGKLGEPSAIALLEQAQMQWYPN
                     GPSDNPFANAIKEILSRHPEAAKPVEPAKTEPNKTEEKTALPPLLTGKVIGITGIPIA
                     GAKLELLTNPLFAKQQPQKAIAAAQTDSNGNYHLAASHQGPAFLICKIQGEPPLEVMQ
                     AVWCSKDNTCIVNIGGQPSLTGSLTLQGNIFAGQRLILSNTVDYTDASFRAEFTTDAQ
                     GNFSLGGIPAGTYYLFHKQADQHLLRLAVFELPLDGSFHFTMDLQPVRVLLDYPANET
                     LPVKAALACAPGILEVPAVISEDGQIIFSNIYAGSYWASVQLSTGVWIQQEVTISSEP
                     AEQVISIAPVLQGNSVLSGRFLSSSTIDLCILNPQRRIRIDLKPAEDGTYLCRDCPAD
                     IYSIAAFFNGQFIEFLQIDLQEQSEIVLDIDPQELIRSRSPLYAVAADTKGLIIGDAQ
                     IWLTGTEELVVTQTTGRGAFLAAPAGRYMLSAACPGYLSAEQSIELRPSMLTAQPDPD
                     NTILIRLENTAHQNTP"
     gene            complement(15879..17363)
                     /locus_tag="PLE88_04915"
     CDS             complement(15879..17363)
                     /locus_tag="PLE88_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOM75843.1"
                     /translation="MALIKLSNSDRTFFQLVADATFTNPFSRQRAEIDRKISGIGGIP
                     DRKQQVSYAVEVIRERLANLDTNKRCRFQDFAGQDRLLMRYTFLFDVYHAYAAAFDAF
                     IAHQQTSASPQKVPFAAAAIKALILRGFTDEEAILYFSMFYQIRRAFYFIDRGLVGKS
                     DCMRKLRMDLWNNIFTYDIRDYEQYMWDKMEDFSTLLEGPTGSGKGAAAAAIGRSGFI
                     PYNPASQEFAASFTRIFISINLSQFPETLLESELFGHTKGSFTGAVSSHQGILSLCGR
                     HGSIFLDEIGEISIPAQVKLLKVLEERLFSPVGSHEQLRFEGRVIAATHQPIERLCRD
                     GRFREDFYYRLCSDCIQVPPLAQRIKENPQELTELVEHFCRQIIGELNTQLVDRVISV
                     IGQRLGPNYSWPGNVRELAQCIRRIILKRQYEGQAVKQSENADALTESIKNGTLTADQ
                     LLNSYCRMLYQRLGTYEETARISGLDRRTVKKRIKQAESHDQFQ"
     gene            17552..17743
                     /locus_tag="PLE88_04920"
     CDS             17552..17743
                     /locus_tag="PLE88_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75844.1"
                     /translation="MEKKALLTLILCGLAAVVIYAAGKTTSGFGPICQTNYVSVNANS
                     EHIDRLCTEMVLSQFIACH"
     gene            17769..19151
                     /locus_tag="PLE88_04925"
     CDS             17769..19151
                     /locus_tag="PLE88_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM75845.1"
                     /translation="MKLLLISPATGNWRQIGRRKFFNGRTFRFSMLSLLTVARLSPPD
                     TDIRLVDEQIETVPLDESFDLVGITCMTATAPRAFELCTYFKQRHIPVVLGGFFPSLN
                     PQKALDYADAVVVGPAFEAWPRLCSDLKAGRLEKIYYGNPAGTIPSTLPREMIRSRKY
                     STPNAVYATMGCRNNCHFCSISAVYKANHYTRPVHEVIDEIRSFKTKFFIFVDDNLTQ
                     NRSYALQLLGQLEPLKKHWITQASIEVAHDQELLTALAKAGCVGLFIGLETFNEQSLN
                     QSDKNFNKPERYKNAIRALHRHGIFVESGVIFGFDEDCVDVFERTLKMLENIGMDAVQ
                     VSVLTPLPGTPLYSQMADRIMDNNFEHYDYRHVVFEPRQMSAEQLQAGADWIIRKFYS
                     PWRIFRRSFRWLWAPNGRSHFIYPFVLNWAYFGRTIVFGIRGYNPARMRFSIADFRFP
                     VLKKAPVRLF"
     gene            19199..19759
                     /locus_tag="PLE88_04930"
     CDS             19199..19759
                     /locus_tag="PLE88_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75846.1"
                     /translation="MKRKMMGLGLVVLSLGAMYLFSSVQPVNGAEKKTLLRVGVYDSR
                     GIAVAYMNSEHFDKILAEKRLALEQAKKDNVSNKVKELEAWGRQHQARAHQQGFGTAP
                     VHECFECIKQQIPAAAKAAGVDVIVSKWECDYLADDADVVDVTMELAKLFNPRPKAYE
                     WIGQLKDHKPISAEEIERLERENPNF"
     gene            19835..20803
                     /locus_tag="PLE88_04935"
     CDS             19835..20803
                     /locus_tag="PLE88_04935"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM75847.1"
                     /translation="MVEQQETMNRETTTLSKPFTVEPMHKVDPAMYPSAVVDYFAHYG
                     LDGDWCEARHLFGSFESAGFTLAAHLYEPSNYIATAVLLHGYLNHSGQFRHLIRFLLD
                     KGFAAAVYDLPGHGLSSGPAAAIESFDQYVQTAQDFLKLVKGYLIGPFYAVGFSTGAA
                     ILTEMMLEKSEGDLGKIVLAAPLIHWTAYEQSKGTYKIYSAFTDKIARFHRKNSSDKD
                     FLVFNKTQDYLHAKHLSLKWVKALFDWNDQIESMNPCDREVLVLQGDKDGTVEWKYNL
                     NLLSRKFPNVRIEMIAGANHELFNEAAEYRQQALALIGKYFQDQLA"
     gene            20847..21926
                     /locus_tag="PLE88_04940"
     CDS             20847..21926
                     /locus_tag="PLE88_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75848.1"
                     /translation="METKRKTKGSWGLRFSVVVLGVVLGVLFYWFLCFIEDDIGTMAG
                     PDWQVIRSRHVAAELDEKQKSLSSEVDALQRKIQVQSEQQQLLNSATNNLQNTINQLL
                     SIQKESVAKNLVFSEKSIQTLQESQAAFLKNQEKNQEYSREIAELTRQKREQEDALKA
                     VNETIKAQEQEAQKDFQEACRRHWLKTAVLKLAFLVPIFLAASFFFMKYRISAYWPLV
                     WAVFLAAFVKIALVAHEYFPTRYFKYIAWAVVTAIVIRILIYLIRMIAAPKKDLLVKQ
                     YQQHYDKCICPVCSKPIRTGPLRYIGAFGKKEHALFGQPAAAQQEPYICPSCGTKLYD
                     KCASCGGIRHILLPYCEHCGAEMGI"
     gene            22040..25486
                     /locus_tag="PLE88_04945"
     CDS             22040..25486
                     /locus_tag="PLE88_04945"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HOM75849.1"
                     /translation="MDTEQSMKLSRSFDWHNATQFLGALNDNIFQGLMIFFLIGLLGA
                     DKKADINAMAGIIFVVPFLLFVPAAGVLADRFSKRNIIVLAKAAELVIMILGCVVFYY
                     NFPMGIWTVLFLMCTQSAFFGPCKYGIIPELVHRDHIARANSYLESMTYLSIVIGAAA
                     GPILAKLSGRNFALAGLACVAVAAAGFLTSTRIAKTKPAGEKSQASLLFVKDVWSTLQ
                     QVRGDRQLMLTIWASAYFMLIGGFAKINLIPYGMEVCGYSDIDSGFLFVMAAVGIGIG
                     CWLAGKLSGRTVEMGIVPLGAIGMAASSIGLGMVSPAGGAVPTPILENPRWSVFILTF
                     LMGISCGLYIVSIHSFIQIRTPVSIRGRVLAASSFLGWVGVLLAALLVKFVCGNLGVP
                     PRWMFLVWGILTFLLAVAAVMIMPDFLIRLIVVLITRLIYRIRVVGMENIPMEGPALI
                     VSNHVSWADAVVLMATQQRRIRFVMDKTIYSNPWLGWVFRLGRMIPISQEPKQIISAI
                     RQARQALEEGYLVCIFAEGMLTRTGMLGRFHAGFEKIVRDTDYPVIPTYLGGLWGSIL
                     SHYHGRLMSAWPQRLPCPVCVHFGRPMPSESSVRDIRWAIEELSVDYFSSRYSNQHSL
                     GQTLIQAARKRWNQPCLSDTAGKRLTFGRTLIAAAALADKIETLTAGQRNLGIFLPPS
                     VAGALANYAAALLNKTVVNLSYTASDSDRACMVETAEIKTVLTSRTFLDKLGFRADTL
                     PGVLFIEDLLAAVSDGDKRKAFLKARFWPADKLARIPRNFSADETAVILFSSGSSGRP
                     KGVELTHCNIQSDIECALTVFKIFKSDKLCGVLPFFHSFGLTCTLWLPLIAGVPACFV
                     SNPLGGKLVGETIAAEKATLLFATPTFLLNYLRHCRPEDFKTIRFIVAGAEKLKTKLI
                     DAFEEKFGIRPCEGYGATECSPLIAFNIPDVEIDGVRQIGTKEGTVGHTIPGLAVKVL
                     DVETGRPVELGQPGLLFVKGPNVMKGYLKQPEITRQVLKDGWYNTGDIVSIDEDGFVT
                     IHDRLSRFSKIGGEMVPHLTIEETAMEKLGFHESVLAVTSLPDEKKGEQLVILYDKRK
                     VDGDKLYAVLTESSLPKLYIPKRENLIGIDEIPHLGSGKLDMIKLRQTAEKYIQMQKS
                     ES"
     gene            25535..>28188
                     /locus_tag="PLE88_04950"
     CDS             25535..>28188
                     /locus_tag="PLE88_04950"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOM75850.1"
                     /translation="MEFFAVLLVFLGIGLAAAGPVAIILAIVLFNKISSIQQRLNRLE
                     GKTEHRGLSMPAVPKEPVWSGQAAVDRTAAKPAVEEPIIPPAQPPMPVQIAEINVSAP
                     VSNQFGVKPSLPYAPAPQPKPTDTGLGRIRPVPTAGKSGWELTIGTTIALAVGTILVI
                     AGVGFFLKYVYEKMLFGPVTRVSMVAVGGIAAIIIGELTRRRGYEIVAKGIAALGFAL
                     LYAAVFGGSRVYHLFGTEWAFALSMIITGSAMTYAVVLNEVFIAFLSLLGGYLSPAII
                     TTGRNLPIPLFSYILVLSGGALGCALVRRWRAVNWIAMVGTYLLYTAWFEKFYTAAQI
                     QTALFWLGVFGCIYLVQPVLYALTRKVAARLEDVILIVVNSAVVFYYLWRMLYADYQG
                     QLGLAAAAWGMMHLVLLVFAMLRCRDDGRLQAVLGVLGSAFIAAAIPLYFDALQPSLV
                     GWSVEAIVLTFVSIRYNSLWSRGMAFLTAAVASAGLFYHLPLHPDADFRLVFNAPFGT
                     WIWVSVSILICHALWRVMASGQNEAGQLLTQVYFVWGRLLLAIGAVLEWYAYCDWHIE
                     FLKQGQAYFLMGVMIVAAILLIGFATRPICPHGDFVRMTAVLTAGAGAIFTAMAMMGV
                     YYDQFRMFLNIPFAMACVFIGSFYFSIRQIRKALPSESHQRQMPDALFMLGLLLIFAV
                     ISEQFYMYWYCRNQYGPTVVDWKMRAHFSILMAWMFYGLVMLAAGIHFNKFSIRILGV
                     IASGLSAAGLFCQLPLHANGAFTFVFNMPFVAWVVVSAGLLAGHVLWRWMKAAVLEES
                     RLAAQIYYAAGVLLLTAGCWLEWHAHCRWQIEPSTAGHSNVLLGGILLSGITMLILLA
                     RPLPPVGDFVQRIGILSALGGAVFTAIAA"
BASE COUNT         6848 a         6556 c         7596 g         7188 t
ORIGIN      
        1 gctatgtgtg tgaaactctc caattataac tgttcaagaa aaggggaggt tgacagaccg
       61 gataaatgat gccgaagaat gcttaaaaac ctgattttac gaacgtcaaa gcagtcaagg
      121 tctgtcaaaa agtatgtctg taagtgttca agtgcatgga aaaccgtata tttacgggat
      181 tttggataat actggggcat ggaatggatt tcttgaccct gcggtagcga attttgtaaa
      241 attgtagatg tcgttaggag gaattatgtt ttttaactgt ttaaatggga aatgtcagtt
      301 agatacacaa tttctgcaac ctaattatcc tattctggag tcgctttttt ctaaaatgca
      361 tatttgaggc gttttatact ggacacctta tttggcaagc ggtgttggct gctggagctg
      421 caaaatgcga ttttggcatc agacagaagc aaaaggccgg gtgttcggga agcggcacaa
      481 atttaattct aatttttata ttcagaaaag catctgaatg gccattacca tataggagaa
      541 aataaacgat ataagcttaa gcttttagga ggaataggac tctttggata tcaacggcaa
      601 tggattcaga tagaacgaaa acagaatatt ttatccattt attgtcttct aatcattacc
      661 ggatttatgc atatattcag agtgttgtga ttaatgaagc cgatgcagat gacatcatgc
      721 aggatacttc gacgataatg tggaaaaaat ttgatgattt ttccgccgga acggattttg
      781 tcgcatgggg tatatcgatt gccaagtatc gcattctcga ataccgcaaa aaaaagaaag
      841 attgcttcct gagtcaggag acgctggacc ttctggccgc tcagagcgaa aagatgatgc
      901 cggagctgga acggaggttt caggccttaa aagactgctt ggggaaactg ccccgcaaag
      961 atctggaatt attaaaattg cgttataccc atagctgttc tgcaaaaatg atagccctgc
     1021 ggattggaac gacaatccac atggtttatc agaatatgtc gagaattaaa atgcttctgg
     1081 ttaactgtgt gcagaagcag ctgcgaaccg gagattttta atgcatcccg atatccaaga
     1141 caatctccga ctgccccggt taatcgaaga aatgcacgat tctgtcatca cgaaagaggg
     1201 aatcgccgac cttgacactc agctgaaacg cgacccgcag gcaaggcagc gctatttgga
     1261 atatgcctac ctccattccg ggctgcacag gtttttaaca tctccggagg ccggctttgc
     1321 cggtcacatt ccgcacgatt cggatttgag cgtgtacaat gatgcactga tggaattggc
     1381 tgccgccgaa accaccgcac cggccgtaga gatttttcat cccaagcagg aaatccatcc
     1441 tgctgtgctg cacggccgga cagagcgggt tgcccgaaca gtcaataagg catcgctgtt
     1501 tgttgccgtt ctgtcgttga cggtcatgct gctgtttctg gcgtatatca aattcgttcc
     1561 ggtctctcat tcccagctgg ttggccggct tgcccgtgtt gcagatgccc agtgggcaga
     1621 tgccagcgga atgattcagg aaggatgcga cctttacacc ggcccgatga aactgatctg
     1681 cggactggcg gagattattc tcgataacgg aaccaaaatc attgtccagg cgcccagtga
     1741 atttgaactg gagtccatga accagatttt tctcaaggcc ggccgcttgg tggtggatgt
     1801 gaacaagcag aacgacaggc actttgtcgt ccggacgatt aacgccgctg ttgtcgattt
     1861 tgggacggaa ttcggggtca gctttgacgg gcgcaatacc catgcttatg tctttcaagg
     1921 cgaggtggaa atccgcaaca gctctgatct cctgagatat gaaaaaaatc tgacagtcaa
     1981 agaagggcag ggaggacagg tcagcagcag cggagacatt tcctcccaga ctcgttctca
     2041 ggaccacttc attcgctcgg acgagtttga agtgcggctc agggcaatcc aaggttcggc
     2101 ttaccatcga tggctggcgt acagtcttca gcttcgcaaa gatccgtcgc tggcggcgta
     2161 ttataccttt gagaaagatc ctgccaatcc cgacctgctg gtcaatgtgg cggcggaaac
     2221 cttcggaagt ctcaacgggc agctctgcag tgccagcggc gaagccccgc ccgcctggac
     2281 gcagggacgg tggccgcaaa aaaccgctct ggcgtttgac cgttcccggc gccagtatgc
     2341 ggaaattgcc gccgatgccc ggctctgcat caacgggccg attactcttg ccgcgtggat
     2401 ttactgcgct gatgcaaagg acggcgggca tattgtgtcc aaccgcctcc cgccgcgttc
     2461 tttctgcaat taccaattgg gctaccacag tcctgtagag atagactggc gccgtaatat
     2521 ccatcttgcc agaaaactgg attcgaatga tgacagcaac caaatagcca gcagacagct
     2581 gcccgaactg ttcggctgga tactggttgc agtgacccat gataatgaaa ccctgaagtt
     2641 ctacctgaac ggacagctgg ttgattcgca gcggtggccg catcaacagg agtttcggga
     2701 ggcacctctg ctgattggtg cggactatgc acccggcgat aactcccggt tcaatgggaa
     2761 aatcgacgaa atcattattg ccaaacgtgt tttcagcggc gaggaaatcg ccgagatgta
     2821 ccgggccggc aggccctgaa aggccggcaa agaaccggct gctatgcgga gaaggatcgg
     2881 atagcagaaa tagaatatgt tgttctcaga agaatagaac gtgcgcaaac aaaagggatt
     2941 tatattaatc aagctgctgg tggtgatagc gattatcgct cagctgctga ccctcattat
     3001 cccgggtctg aatatggcca aacgaaaggc ctcaatggtg gccgacttgg cgaatgtcaa
     3061 aagcacctct ctggcatggt atatgtatca gggagaaaat aacggtctga tttgttcatt
     3121 gacccctcaa aacgcctatc catgggtcaa aaatccagtt gatacggccg gaaacacccc
     3181 ttcgctgacg caggttaccc ccattgtaac ggatgaggat gaaaaacggg gcatcagcaa
     3241 gggcgtcctg tttgaataca tcaaaaacgc tgacgtattt cactgcccga tggatcagcg
     3301 gaaaagctta tatgacctgt ccaatatctt ccgcacgtat tctatgccgg cttacctgaa
     3361 ctggtccggc accctgaccg gcactaatga aatccaccgt ttcggaaaga tcgtgggtcc
     3421 ggggacaaaa attatgctgg tcgaagaggc ggatatgcgg aattacaaca acggcgcctg
     3481 gagtttcggg gctccggaat ggacaggggg cggattggag tggtgggatc ctctggcggt
     3541 aacgcacggc gacggcagca ccttgggatt ttgcgacggg cctgcagaaa atcataagtg
     3601 ggtggatccg tttaccaagg accggcccaa caagctggca aagactggaa ccaccaacta
     3661 cggccgagaa gctcccccgg cagggcacac aacggatata caatatattt acagcggctg
     3721 gggataccgg aagaaataac cggatgaata acagcggcag gattgataga agacaatgcg
     3781 attagcagca aacagattcc gggagaaatt gaacagtgga agcctgcata ggatttaatg
     3841 aaatcggcgt gaagtcgatg aggctgcagg aattacctaa ccaatcattt tttttgaaag
     3901 ggtcatgaga tggaaaatgg aaagagggta ttgttgggtg tgtttcttgt gatggtcggg
     3961 ctgctgcagg cagtcaccat cgaatgggtg tcggatgatt acggctcaag tttcgaccag
     4021 ccgtggatca atctgctgac cagtcaggga tacacggtta ctgctcgtgc caaccagtac
     4081 taccgcacgc tggatgcggc gaaaatcgca gcgctgaacg cggcggatct ggtcatcatc
     4141 agccggaaga catccagcgg cgattatgcc agtaacagca cggaagtggc acaatggaac
     4201 ggcttgacgg cgccggtgct gtgcatgagt ggatttctgg cacgaagcag ccgttggaaa
     4261 tggctgaacg ccgaccaaaa cgaggcggac gcaattatga aggttcttga tccatcccac
     4321 ccgttttttt ggggcgtgcc cttggatgca aacaatcagg ttgacaccat tgccaccggc
     4381 gtggcaagcg taattgccac gaccaatccc ggcaattcca ccgttctggc gacccgtgcc
     4441 tcggaccaat acatctggat tgccaaatgg gataccggcc aagagtatta tccgggttcg
     4501 ggacagtatg ctggcggacc gcgtgtgttc tgcggcatgg ggcaggatgt ttccggttcg
     4561 gctttcaacg ttactgaagc cggcaaaagt ttgtttttga atattgtgta tgaaatgagc
     4621 ggcgctgcgt ggaaccgcaa gccgctggta aacgccggac cagaccgcat cgcctatatc
     4681 ggtaatgctg tcgcgctggc cggatctgcc atcgatccgg aagcgacggt gatgaccata
     4741 agctggacgc aggtttccgg accggcagcg gcctccatcg ccgatgcctc cgcggcggct
     4801 acgactgccg ccttcagcgc cgccggaaca tatgtcctga aactggaagc cagcgatggc
     4861 gagctggcaa catcggatac aatgaccgtt tatgtcagag atcccgccaa ggacaccctg
     4921 attgcccact gggatttcga cggtctgccg gaccccaata ctctgattga tgtcagcgga
     4981 catggttttg acggcatcta ccacagaacg actcccggcg agccgaatgt tgtccccgga
     5041 catattaccg gctctctgaa ggcagctgat tttatggaaa tcagctattg ggaaatcccc
     5101 aatgcgcggg caggcgaccc gaattttaat gatattcaaa cgggcgccac atttgccgtc
     5161 tgggccaagg ccgatgcgca aggagcagta agtacgccgg ttatcttcgg ctttgggatt
     5221 gatgctgtga gatttcagat taatgcaaac cggtggaatc tagttcagac gggattggac
     5281 gtgttcagcg tccgtccggt ttttgatggc ttctggcatc atgtcgctgg tgtctatgac
     5341 ggcgtcaatg cccagttcaa gatatatgtt gacggcgttt tggacgggac cacagctact
     5401 ccaagcggct atctgctgac caccggggct tttccgccgc agatcggaaa ccgcgcggat
     5461 gctgtccgta tgtggcccgg gcagatggat gacctgcggg tttacaacta cgctctcagt
     5521 gacgcccaga ttgccgcact ggccgcagaa ggcgacttga ttccatacct gacggcaggc
     5581 atagatcaga ccgttcagta cacaggggct ccggttccgc tggatgcgac gcttattgtc
     5641 aatgacggca ttcccgcccc gatggcctta tcgtggtcgg ttaccaaagt gccggcaggc
     5701 gtggatcccg aggatgttat tttcagtgac aagacagctg aagatccgct ggtaaccttc
     5761 ccgactgttg ccgggactta tgtcctgaag ctgacaggca gcgacggcgt tgttgaagtc
     5821 agtgatgaag tcagcctcgt aatcctgatt ccaacctgtg ccgatattct ggctgagggt
     5881 ctgggaatgg ccgcagatct gagcggtcct gccggagtgc cggattgcta tgtcaatatg
     5941 tacgatctgg cagcgatggc ggccagctgg ctgacatgca ataacccgga agataccggc
     6001 tgtatatggc cgtattagaa tgattgatcg gagggaaagc cctgtttttg ggggctttcc
     6061 cttctgttcg gttatgttgg ggaaacatcg caaacaggtc ggcaagattg tttgcagccg
     6121 tttttttggg caacggcaga tgagccgtat gggggctgga agccggatag ttgctgtcac
     6181 gatgcagctg cggcgtatca tcgttgtcat agtttaactc cttttgaatg gttcggttta
     6241 ggaacggcaa tgagatgaaa aacgcaaaga gactattgtt cgttgtagta gttgccctga
     6301 cgggcctgcc gcaggcggct actatcgtat gggtatcgga cgactacagt gctaatttcg
     6361 accagccgtg gatcaatctg ctgacgagtc agggctacac agtccttacg gcagctgacg
     6421 gcgcgacgac atactggcga acgctggatg aggcgaagat cgcagtgctc aacgcagcgg
     6481 atctggtcat tgtcagccgg aagacctcca gcggtgaata cgccagcagc agcacggaag
     6541 tggcgcagtg gaacggcctg acggcgccgg tgatgtgcat gagcgcttat ctgacacgaa
     6601 atgtccggtg gaaatggcta gacggcttgc aaagcgaagc ggatgcgatg atgcaggctg
     6661 ctgttgcgtc agatgaaatt ttcaggggag tgcctctgga tggcagcaat caggttgatg
     6721 tgattaattc aggcgttgcc agcacggtta tcacagccgc tgccggcagc ggcacggttc
     6781 tggcaacccg cgcgacggat cagtccgtct ggattgcccg atgggatgcc ggagcggagt
     6841 tttaccccgg ttcagggacg gttgccggcg gaccgcgcat gttttgcggt ttaggtcagg
     6901 atgtttccgg tgcggcgtac aacattaccg aagccggccg gactattttt ctcaatgccg
     6961 tgcagtccct ttgctttcgc aaagggatcg tcggttcggt tgcagaaacc ggcggcagca
     7021 ccgaagtatg ggaaaaaccg gccatttcgg atacattcag cgtggtttta aacaaattgc
     7081 cccttgccgc agtggatgtg actgttcagc ccgtttcaaa tgctgccgat attcaaattg
     7141 acggttctgc cggaccgggc gagccgattg tgctgacctt tacaccggcc agctgggatg
     7201 tcccccaaac tgttgcagtc cgcgcagtgc cggatgaact ggaagaagga accgaatacg
     7261 ccgcaatcga attcaggatt cacagcaccg atcccgattt taacgacggc cttatttttc
     7321 cggtacagat taccgtattt gattccccaa tcaatgactg cccaacagca gacctgaatc
     7381 atgattgccg tgtggatatc ggcgatttgg cgttgttagc cgcggcgtgg ttaggagatc
     7441 cggcaagccc ggcaaactta tatcctgaca gcattattga tttaaaagat ctgtccttat
     7501 tttcacaaaa ctggctcgac caagaaggcc cggttgtcat cagcgagttt atggcctcca
     7561 acggcgttac cataagcgac ggcgataatc agtcctccga ctggattgaa ctgtataaca
     7621 tgacagacgc ggccgttaat ctggagggct ggtatttaac cgatgatgca gcacggctga
     7681 cgaaatggcg gtttccggcg ggctctgtaa ttcagccgta cagctatttt ctggtttttg
     7741 cctccggcaa gaatgatctg ctctatccgt acattgactc cagcggtttt ctgcatacca
     7801 atttcaatct gaatgcctcc ggcggttatc tggcattgac tggcccagac ggtcagacgg
     7861 ttgttcatgc ctacccatcc taccctgctc aggaaaagga tatttcctac ggcctatatc
     7921 ggcttcaaaa tcggtatttt gttcaaccca cgccgctgtc agccaacagc gcggtgtatg
     7981 aagggctggt ggcggatacc aaattctccc gcaatcgagg cttttacacg gcgccgtttg
     8041 aagtttctat cagctgtgca acgccggggg cggcgattca ttatacaacc gacggcagca
     8101 cgcccaatga aagcagcccg gcttataccg gtccgattgc aatctccgcc acaacctgtc
     8161 ttcgtgcggc ggcctttaag ccgggatggc gtccgagcaa cacggatacg cagacatatt
     8221 tatttattca ggatgtaatc agccagaccg ctccgcccgg atacccggct gtatggaatg
     8281 gctaccccgc cgattacgag atggatccgg atatctgcac gaatcccgct tacgccgatc
     8341 tgatcgacga tgccttgctg tcgctgccga cagtgtccct tgtaaccgac aagaaaaacc
     8401 tgttcgatcc ggcgacaggg ctttatctga atacaacaat ggagggcgat ttgtgggaac
     8461 ggccggtttc aatggagttt gtcgatccca gcggaaccga aaagagtttt caggaggatt
     8521 gcgggctgcg gattcagggg ggcgccagcc ggaatccaaa caactcgccc aagcattcgc
     8581 tgcggctgct gtttaaatcc gcctacgggg cgtccaaact gaattacccc ttcttcggcg
     8641 atgatgcagt cgattccttt gatacactgg tgcttcgcgc cgactacaat aattcctgga
     8701 ttcactggga tccgcagcag cgtctgcggg ggcagtacct gcgggaccag tttgcacgcg
     8761 atacccagct tgcaatgggg cggatgtctg cgcacgggcg ttttgtgcat ttgtatatca
     8821 acggccttta ttggggcctg tacaatgcga cggaacgtcc ggaagcatcg tttgcggcga
     8881 gttattacgg aggcgaaaag gaagaatggg atgcatttaa tgccggcgtt gttgtcgatg
     8941 gcgatgatgc agcgtggaaa caggccctca gcatcgcctc cgcaggcgta tcggatgcag
     9001 ccggatatgc cgctctgtcg gaatatgtgg acattccaaa cctgattgat tatatgctcg
     9061 tcaactttta cggcggcaat caggactggg gcgccgccaa ctggtacgcg gcgggcagaa
     9121 aaaccggcac cgaaagattc aagtttttct gctgggatgc cgagcgaacc cttgaagatc
     9181 cagttgctca caatgtgacc ggcgtcaatg ccgccaacag tccggcctac ttttacgcac
     9241 agctcagggc caatccggaa tttcgaatgc agtttgccga tcaggcgcac cgccatcttt
     9301 ttaacggcgg cttgctgacg cccagcgcct gtatcgcgcg atggatggaa cgagccgggc
     9361 aaattaatac cgccgtcatt gccgagtcgg cccgctgggg ggactaccgg cgggatgtcc
     9421 atgtgcgggg cgaggctttc ctgtatacac gcaatgacca ctggatagcc gaacaaaacc
     9481 gactgacagg aagttatttc cctgtccgca ccaatactct gattcagcaa ttgcggaatg
     9541 ccggattgta tccggcggtg gatgccccga cgttcaatat caatgggaca tatcagcacg
     9601 gcgggtatgt cggcaatccg tctgcgctga cgatggacgt gacaggtaca acctcttatg
     9661 tgagcacaga acttgtttca gaaggcgctg cggttcgcgt ctatatgcct gcagacaatt
     9721 cgctgggctt gacatggacc ggacaatctt tcagtccggg tgccgactgg acagacggtt
     9781 ccaccggcac gggcgtcgga tatgatctca gtacgccgta tgatgctctg attcagacgg
     9841 atgttcagtc tctgatgtac caaaaacagc cgtctgtgta ctgcaggatt gcctttgagg
     9901 tatccaatcc gtctgttgtc agcagtttga agctgcagat caaatacgac gatggtttca
     9961 tcgcctatct taacgggcag gaagtatgcc gaagctccaa tatttcctat gagattccgc
    10021 cgactgcagc tgctgccagc catacggcat cggcatttga agaattcgat attacttccg
    10081 ctgttggaca attgactgcc ggaaccaata ttttggcgat tcacggcatc aacagcagca
    10141 ggacaagcag tgatttgctg attttgccga agctgattgc ttctgtcggc agcacggtca
    10201 ttccgcctat ctggtacacg acagacggga ctgacccgcg ccagtctggc ggggcggtgt
    10261 ctccttctgc ctcgctctat tccggagctg taacgcttac agagtcctgc tgtgtgaagg
    10321 cccgtgcgct gaccaacggc acatggagtg cgctgaatga ggcagcttat gccgtggggc
    10381 cggtggctga aagtctgcga attacggagc tgatgtacca tccggccgat cccaactgcg
    10441 agtatatcga gtttcgcaat atcggcagcc agacgattaa cgtggcgcgg gtgcagatga
    10501 cgcggggggt gacttttaca ttcccgagtc tgtcccttgc tccgggcgag tatgccgttg
    10561 tggtgcagaa tctggccgca tttgcgggcc gctataatac cgccggcatg acaatcgccg
    10621 gccagtatgc cggaagtctg gaaaacgccg gagaaaaaat aaagctcgtt gacgcggccg
    10681 gacaggtcat tcacctgttt gattacagcg atgactggta caaattcacg gacggttacg
    10741 gattttcgct gactattaaa gatgaaacca atccggatct ggcactatgg gatcaaaagg
    10801 ccggctggcg tcccagtgcc gtctccggcg gatcgccggg gaccggcgat gcaggcattg
    10861 tcccgccgct gggagcgatt gttattaacg aactgctggc gcattcccac gctgccgaac
    10921 cggactggat tgagctgcac aacaccactg cccatgacat caacatcggc ggctggttcc
    10981 tttccgacag cgatgccgat gacctgacta tcaggaagta tgaaatcgca ctggatacgg
    11041 tcatcccggg caacggttat ctggtcttct atcaggacca gactttcggc aataccaccg
    11101 atccgggctg tcatattcct tttgcgctga gcgaaggcgg ggaaacggta tatctgcatt
    11161 ccggcctcgg cggcgtgctg accggctatg tggaaaagga aagctttggc gcatcggaaa
    11221 gcggagtggc ctttgggcgg tattacaagg ccagcacaga cagctataac tttgtggcaa
    11281 tgagcgtcaa tacgcccggg caggggaacg cataccccaa ggtcggtccc attgtgataa
    11341 gtgagatgat gtataatcct ccggccggcg gttctttcga ccatgatgat tatgaataca
    11401 tcgaattgcg aaatattgaa gacgagacga taacgctgga agaacatgat gtcctgctgg
    11461 atatcgatgt gccatggaag tttaccaatg gcattgatta caccttcccg cttggggtat
    11521 ccattgctcc cggcggctct attgtagtag tgaagaatcc cgctgcgttc agtgaacgtt
    11581 atccggacgt tccagccggc gtcatttatg gtccctattc ggggaaactg gacaatgggg
    11641 gggagaagct ggaattagca atgccgggtg atgaagaagc ggcggtaagg tattatatct
    11701 gtgtggatcg ggttaattat agtgacggat cagccccggg agacctctgg ccggtagcag
    11761 cagatggaca aggcggttcg ctgcaccgga agaatgacca attatatggc aacgatgtcg
    11821 ccaactggca ggccgcttta ccgagtcccg gtaattgacc tgctggttgc tgatctttga
    11881 aaagataacg gatcgccttc catcttgtgt aactgttatt attaagggct gttcgacaca
    11941 acaaacaatc agaattgttt tttgagaagt tcaatagata ttgagcattt cttttatatg
    12001 gcaaaatggg gggacagtcc caataggttt tgccgggctt actggagatc atcagcatgg
    12061 gattgaccac cgtaaacatg ggctgacaat gctttatgcg ggaactttat ggcaggtttc
    12121 ttaaattgaa cctgacattt gaaacgaaat cttctggaaa agtttaatct atcagctcgt
    12181 gctcgattta ctcaagagtt ttccctttgg tttcaggccg ttttgaatag atgatgatta
    12241 aacctataat tgtttgacgt caagcagaaa gcgggacagc cccccgcaga gggataaggg
    12301 gctgtggcgg tagatggcga ctctatctgt ccgatgctgc cgatgggggc agactcccta
    12361 tgagggaatt atatgtgaat atatgtaaat acctgtatct atggaaaatt tggctaaaaa
    12421 tccatactca cagtctgcgc ctatggataa tgagctaaaa atccataggc gcaggtattt
    12481 acaggtctca gatgcaatcc caaaccccgc cgtcaggggc tatacgagga ttttgagcgg
    12541 agtttgtttc gagcaggcga atatgatgac gcattggaat tttatgaata aaatgagtta
    12601 tgacggccct tcgggcctga taaggaaggg gctgttttta tcagcgtggg cactgcccac
    12661 cctacccggc tattcagact gcttctgtcg gctaccctct catccaactt ggatcaattt
    12721 tcggggagca ggccacacat ggggctgaac tggttaaaat gaagggaacc tctaataatt
    12781 tgatttttga ggatcaaaat ctcagttaac gttttttagg taaatttttg aacacagtat
    12841 tccaaaattt cggtttttcg ccgtcaataa acggacaatc gacccgctta ccagcggttc
    12901 gccggggcgt tttcgcccat ccgacgcacc gggggcgctt aggccgtcgc caacagacag
    12961 aaccggtcta tattatacgc cagattccgc aatccaatct tggccgcagc ccgcgtggta
    13021 ccgatcgcgc gcatgatcaa gcatccggcc cgctggctct ttttttcgga aataaccgca
    13081 aatctaaaat ccgctgtgta gttgctgaat tttcagaatg ctatgaaaaa tctatcttct
    13141 atggcgtgtt ttgatgggct gtattttcta aacgtatgag tattgtattg tcaggatccg
    13201 gctgtgctgt cagcatggat ggccttagtt caattgattg ttctgccgat agataccccg
    13261 ggcatgctgc tgagagcatg taacgtccag ctggagccgc caaaaaggcc ccgcggccgg
    13321 ttgtttgggt aacaaccaat tcttctgtgc ctgtcagcca gatctgggcg tctcctataa
    13381 tcagcccctt ggtatccgcc gcgacggcat aaagcgggct tctcgaccgt attaattcct
    13441 gcgggtcaat gtccaaaact atttccgact gttcttgcag atctatctgc aaaaattcga
    13501 taaattggcc attgaagaaa gcagcaatac tatagatgtc cgctggacag tcacggcata
    13561 gataagttcc atcttcagcg ggttttaaat cgatgcggat tcgccgttgg ggattgagga
    13621 tgcatagatc tattgtgctg gagcttaaga accgccctga aagaacagaa tttccctgca
    13681 ggaccggggc aatggaaatg acctgttcag ccggttcaga tgatatagta acctcctgct
    13741 ggatccagac accggtactc aactgaacgc ttgcccaata ggagccagcg tagatgttgc
    13801 taaatataat ctgtccatcc tcggaaatga cagccggaac ctctaaaatc ccgggggcac
    13861 aagccaaagc ggctttcacc ggcagcgtct cattagccgg atagtcaagc aaaacacgca
    13921 ccggctgcaa atccatcgta aagtgaaaac tgccgtctaa tggcaattca aaaactgcca
    13981 gccgcagcaa atgctggtct gcctgcttat ggaaaagata atatgttcct gctggaatgc
    14041 cgcctaagga gaaattacct tgagcatcgg tggtaaattc ggcccgaaaa gatgcgtctg
    14101 tataatcaac ggtattgctt aaaataagcc gctggccggc aaagatattc ccttgaagtg
    14161 tcaaactgcc ggttaaagac ggctggcctc caatatttac aatgcaggta ttgtctttgg
    14221 agcaccacac ggcttgcatg acttccaaag ggggctcgcc ttgaattttg cagattagaa
    14281 aggcaggccc ttggtgggat gcggcaagat ggtaattccc attgctgtct gtctgggcag
    14341 cggcaatggc cttttgcggc tgctgttttg caaaaagggg attggtcaac agttcaagct
    14401 ttgcaccggc aattggaatc cccgtgatgc cgattacctt gccggtcaaa agcggcggca
    14461 gggcggtttt ttcttctgtt ttgtttggtt ctgtttttgc aggctcgact ggtttggctg
    14521 cttccgggtg ccggcttaaa atttctttaa ttgcattggc aaaaggattg tcggatggac
    14581 catttgggta ccattgcatc tgggcctgct cgagcagagc aatagctgag ggttcgccaa
    14641 gtttgccgat aaaatgagcc gctgccatct ggccggcgaa atgaccggat tttaaaatct
    14701 ctaaaagcgc atccagattg cctgcctgac ccagttcgaa aacctgcttg agtttttctt
    14761 tttccggatc attgtgttcg ccgactgatg cagtcggctg ttcaattttt ttcggttcct
    14821 tttccgccga aggattagat acagctgata ccgcaggggg agcggaaggt ttctgataaa
    14881 gactccgcaa aatgcctgcc gctgcggcaa gaataatgaa gataacagca atgcctataa
    14941 tggcggccgt acggctcatg agtcttttgg ggggctgatt ttgagcggcc tgtcgggcag
    15001 cttcgagcat cgacgacaag atagattctc tttgggctgg actgggtgtc tgatatatcg
    15061 cggtggaact cagcagtttt tccaggttgc tgtccagcag ttcaatactg cctgtggcac
    15121 tggattcgga aaaagccgga ttggcagctg ataacagctg tgtaaaaatc ctgcgaaagg
    15181 aatgccgaca gcggtataga cgattattga cttcttcaat acgaatgccc atctgttcgg
    15241 cgatgtgctc cggcatatcc agatgatggt agcggtaaat catcagctcg cggtcattgg
    15301 catccatttc tgccagcgct gcctgcacca attcctgcac aaacgggtag gcggctgttg
    15361 tatcgggtat tgcttggaat cgcagacctg ccagtgcctg cagaatatgc tctggcagtt
    15421 gagaccacgc ccatggacgc tggggctgaa tctgccggcc ggccattccc tcgctgagag
    15481 ccagcttggc ctgttccatg agccattgag acaggctttg ttttgcggta tcaaaagaag
    15541 taatgtttag aatgattcgt cggaatgtca ggtctgttaa ttcaagagca atctgcggat
    15601 cagcacccgt ctgataataa agccaggtat agatccatgg cccgaaatgt tcaaaaaatc
    15661 tctcaatggc cgatgggtct gcctgatgga gttgaaaaat gcgtttttta ttccaatatg
    15721 cttccattct gctggtgtta tctactgtaa agggcactat tttgcctaca ttcgtataac
    15781 agcatttggc gtctgtcttt tctcaagata gcgtaaattt agtttcgtga aaacatatca
    15841 tatatatcgg caatcaataa taaaaaattg ccttaaaatc attggaattg atcgtgcgat
    15901 tctgcctgtt tgattctttt tttaacggtt cgtctgtcaa gaccagatat ccgggcagtt
    15961 tcttcataag ttccaagccg ctgataaagc atgcggcaat agctgttcag caactggtcg
    16021 gccgtgagtg tcccgttttt aatgctttct gtcagggcat cggcattttc cgattgcttg
    16081 acagcttgac cttcatactg tcgtttcaag ataatccgac ggatgcattg agccagttca
    16141 cgcacattgc ccggccaaga ataattgggt cccaatcgct gtccgataac cgaaataaca
    16201 cggtccacca attgtgtatt cagctccccg ataatctgcc ggcagaaatg ctcaactaat
    16261 tcggtcagtt cttgcggatt ttctttgatg cgctgtgcca gcggagggac ctgaatgcag
    16321 tctgaacaca gccgatagta aaaatcctcg cgaaatcggc cgtctctgca cagacgttcg
    16381 atgggctgat gagtagctgc aatgacacgg ccttcaaaac gaagttgttc gtgactgccg
    16441 acaggactga acagccgttc ttcaagtacc ttaagcagtt taacctgtgc cgggatgctg
    16501 atttcgccga tttcatccag aaaaattgag ccatgccggc cgcagagact caaaatgccc
    16561 tgatgagagc tgactgcacc ggtaaaactg cccttggtat gcccgaacaa ttccgactcc
    16621 agcagggttt cgggaaactg tgacagatta atcgagatga atatacgggt aaaactggcg
    16681 gcaaactctt ggctggccgg attgtaggga atgaagccgc tgcgtccgat tgcagcggct
    16741 gcagcacctt taccgcttcc ggttggacct tccaaaagtg tcgaaaaatc ctccatttta
    16801 tcccacatat attgttcgta atcgcggatg tcatacgtaa aaatgttgtt ccataaatcc
    16861 atccgaagct tccgcatgca gtcactttta ccgaccagac cgcgatcaat aaagtaaaag
    16921 gcccgccgga tctggtagaa catggagaag taaagaatgg cttcctcatc cgtaaatcct
    16981 cggagaatca gggctttgat tgctgccgct gcaaaaggga ctttctgcgg ggatgctgag
    17041 gtctgctgat gagcaataaa ggcatcaaag gcagccgcat aagcgtgata aacatcgaac
    17101 aggaaagtat agcgcatcag gagcctatcc tgcccggcaa aatcctgaaa gcggcagcgt
    17161 ttattagtgt ctaaattagc caaacgctcg cggataactt caactgcata cgaaacctgc
    17221 tgtttccggt caggaatgcc gccgattccg ctgattttac ggtctatttc tgcacgctgg
    17281 cggctgaatg gattggtgaa tgtcgcatca gcgaccaatt gaaaaaacgt ccggtccgaa
    17341 ttggaaagtt tgataagtgc catacataaa aggtacagca tttatgcaat aaatgtcaat
    17401 aaaaaaccat gaataattta tctgagtcta tttgatactt tgttaactta ttgagaaatc
    17461 tatgcttacg gtattttggt ttatctggca cacggattgt actattaaca agcaatggaa
    17521 ataagaattg aaaaatgaaa gggtacaagc gatggaaaag aaggcgttgt taactttaat
    17581 tttatgcggt cttgctgcag ttgttattta tgcggctggg aagacaactt ctggcttcgg
    17641 accaatttgc caaaccaatt atgtctctgt aaatgccaat tctgaacata ttgatcgctt
    17701 atgtacggaa atggttctaa gtcaatttat agcctgtcat taggatggga aagaaaaagg
    17761 acaaaacaat gaaactatta ctgatttcac cagcaacagg caactggcgg caaatcggcc
    17821 gccgaaagtt tttcaacggc agaacatttc gtttttcgat gttgtctctc ctgacagttg
    17881 ccagattgtc ccctccagac accgatatcc gactggtcga tgaacagata gaaaccgttc
    17941 cgctggatga atcctttgat cttgttggaa ttacctgtat gacagcaaca gcaccgcgtg
    18001 cgtttgaatt atgcacgtat tttaagcaaa ggcatattcc agtggtgctg ggaggttttt
    18061 tcccgagttt aaatccccaa aaggcattgg actatgctga tgctgttgtc gtcggaccgg
    18121 cgtttgaggc ttggccgcgt ttgtgcagcg atcttaaagc gggccggctt gagaagattt
    18181 attacggcaa tcctgccgga accatccctt cgacgctgcc ccgagaaatg atccggtcaa
    18241 ggaagtattc taccccgaat gcagtgtatg caacaatggg ctgcagaaac aattgtcatt
    18301 tctgctctat cagtgctgtt tataaggcca atcattatac ccgtccggtt catgaggtaa
    18361 tcgatgaaat ccgttctttc aagaccaagt tctttatatt tgttgacgac aatctgactc
    18421 agaatcgcag ctatgcactc cagctgctcg ggcagctgga gccgctcaaa aagcattgga
    18481 ttacacaggc ctctatcgaa gtagcccatg accaggaact tttgacggca ttggccaaag
    18541 ccggatgcgt cggtttgttc atcgggctgg aaacgtttaa tgaacaaagt cttaaccaat
    18601 cggataaaaa ttttaataag cccgaaagat ataaaaatgc gattcgtgcg ctccacagac
    18661 acgggatttt tgtggaatcc ggcgtaatct ttggttttga tgaagactgc gtcgatgtat
    18721 ttgaacggac attgaaaatg cttgagaata tcggcatgga tgccgttcag gtttctgttt
    18781 tgacgcccct gccgggcact cctttataca gtcagatggc agaccgtatt atggataata
    18841 attttgaaca ttatgattat cgccatgtag tttttgagcc ccgtcaaatg tcagctgagc
    18901 agctccaggc cggcgcggat tggattatcc gaaaattcta ttcaccatgg agaatatttc
    18961 ggcggtcctt tcgctggctg tgggccccga atggacggag tcattttatc tacccatttg
    19021 tcctcaactg ggcctacttt ggcaggacaa ttgtgtttgg gattagagga tacaacccag
    19081 cacggatgag attttcaata gccgattttc gatttccggt tttgaaaaaa gcgcctgtta
    19141 gacttttttg aaagtcgata tagaaaagat aactatgaaa acgaaaggaa aggttgaaat
    19201 gaaacgcaaa atgatgggtt taggattggt tgtgttatcg ctcggagcga tgtatctgtt
    19261 tagctcggtt cagccggtga acggcgcgga aaaaaagaca ctgctgcggg tcggtgtcta
    19321 tgacagccgg gggattgcgg ttgcatatat gaattcagag cattttgata aaatcctcgc
    19381 ggaaaaacgt ctagcactgg aacaggcaaa aaaagacaac gtttccaaca aggtcaaaga
    19441 attggaagca tggggccggc agcaccaggc aagagcccac cagcagggct ttgggacagc
    19501 gccggttcat gaatgttttg agtgcatcaa gcagcagatt ccggccgctg ctaaggccgc
    19561 cggtgtggat gttattgtca gcaagtggga atgcgattat ctggcagacg atgcggacgt
    19621 tgtggatgta acaatggaac tggcgaagct gtttaatccc cgtccaaagg cgtatgaatg
    19681 gataggccag ttgaaggacc acaaaccgat cagtgctgaa gaaatcgagc gtctcgaaag
    19741 agaaaatccg aatttttaga aaaattcatc agaaaaggaa ggattgttgc cgcaaagaat
    19801 gagtagttgc tttttgtgag gtgttgtagt acaagtggta gaacaacaag agacaatgaa
    19861 tcgggagacc acgacgttgt ccaagccatt tactgttgag ccgatgcata aggtcgatcc
    19921 ggctatgtat ccgtcggcgg ttgtggatta ttttgcccat tacggtctgg atggcgactg
    19981 gtgtgaggca aggcatttgt tcggctcgtt tgaatctgcc ggttttacct tagcggcgca
    20041 tctgtatgag ccgtccaact atatcgcgac ggcggtcttg ctgcacggct atctcaatca
    20101 cagcggacaa ttccggcatt tgattcggtt tcttctggat aagggatttg ctgccgccgt
    20161 ctatgacctg cccggccacg gtctgtcatc cggcccagcc gcagcgatcg aatcttttga
    20221 tcagtatgtc caaactgcac aggattttct aaaacttgtg aaagggtatt taatcggacc
    20281 gttttatgct gtcggtttca gtactggtgc ggcgattctt acagagatga tgctggaaaa
    20341 atccgaagga gacttgggaa aaattgtcct tgccgctccc ctgatccatt ggacggctta
    20401 tgaacagtct aagggaacgt ataaaatata cagcgccttt acggacaaaa ttgcacgttt
    20461 tcaccgtaaa aattcctcgg ataaggactt tttggttttc aataaaacgc aagactatct
    20521 gcacgccaaa cacctgtctc ttaaatgggt caaagccctt ttcgactgga atgatcaaat
    20581 tgaatccatg aatccctgcg acagggaagt tctggttttg cagggcgaca aagacggcac
    20641 ggtggagtgg aaatacaatc tgaatctgct cagccggaaa tttcccaatg tccgtatcga
    20701 aatgattgcc ggcgccaacc atgagctgtt caatgaagcg gctgaatacc gacagcaggc
    20761 gctggccctt atcgggaagt attttcaaga ccaattggca tagtgagaac aacagctata
    20821 ttaaatgcaa tacaggagaa caggttatgg aaacgaaacg gaaaacaaaa ggatcctggg
    20881 gactgcggtt ttcagttgtt gtgctgggag ttgttttagg tgtactgttt tactggtttt
    20941 tatgctttat cgaagatgac attggcacga tggcagggcc cgactggcaa gttatccgga
    21001 gccggcacgt ggcagcggaa ctggacgaaa agcagaaatc gctgagcagt gaagtcgatg
    21061 ctcttcaacg caagatacaa gtgcagtccg aacaacagca gcttttgaac agcgccacaa
    21121 ataatctgca gaatacgatt aaccagctgc tttctattca gaaagaaagc gtagcgaaaa
    21181 atcttgtgtt ttcggaaaaa agcattcaaa ccctccaaga aagccaggct gcatttttga
    21241 aaaatcagga aaaaaatcag gaatacagca gggagatcgc cgagctgacc cggcaaaagc
    21301 gggaacaaga agatgcgctg aaagctgtga atgagactat aaaagcccaa gagcaggagg
    21361 cacagaaaga ctttcaagaa gcctgccggc ggcattggct caagacagcc gtattgaagc
    21421 tggcgtttct ggtgccgatt tttctggcag cttccttttt ctttatgaaa taccgaatca
    21481 gtgcctattg gccgctggta tgggctgtgt ttctggcggc atttgtcaaa attgccctcg
    21541 tagcacatga atatttcccc acccgatatt ttaaatatat tgcttgggct gttgtaacgg
    21601 caattgtgat aaggattctc atttatttaa tccgtatgat agctgcgccg aagaaagatt
    21661 tgcttgtcaa gcagtatcag cagcattatg acaaatgcat ctgccccgtt tgcagcaagc
    21721 ctattcgaac ggggccgctt cgttatatcg gtgcttttgg caagaaagaa catgctctgt
    21781 tcggacaacc ggcggcagcg cagcaggaac cctatatttg cccctcctgc ggtacaaaat
    21841 tatatgacaa atgcgccagt tgcggcggca tccgtcatat ccttctgccg tactgtgaac
    21901 actgcggcgc agaaatggga atatagatgc ggtgattggg aaatatgtca aatttagaag
    21961 gtgaaaattg gattaaatca gcttaaaata cacaaaaaat atctgcaaat ttggttattt
    22021 cgtaaatcag ggtaaggcct tggatacaga acaatcaatg aaattaagcc gttcatttga
    22081 ctggcacaac gccactcagt ttttgggcgc tctcaatgac aatatttttc agggtctgat
    22141 gatatttttt ttaataggcc tgctgggagc agataaaaag gcagatatca atgcgatggc
    22201 cggcattatt tttgttgtac cgtttttgct gtttgtgccg gctgccggcg tactggctga
    22261 tcgattcagc aaacgcaaca ttatcgtgct ggcaaaggcc gccgaactgg tgattatgat
    22321 actgggctgt gtggtattct actacaattt tccgatgggg atttggactg tcttgttttt
    22381 gatgtgcacc cagagcgcat ttttcggacc ctgtaagtac ggtattattc ctgagttggt
    22441 ccatagggat catatcgctc gggccaacag ttatctggaa agcatgacat atctgtctat
    22501 tgttatcggt gctgccgccg gaccgattct ggcaaagctc agcggccgca attttgcttt
    22561 ggcaggattg gcttgtgtgg ctgttgctgc ggcgggtttt ttaaccagca cccgcatagc
    22621 caaaacaaaa ccagccggtg aaaagtcgca ggcatcgctg ctgtttgtca aagatgtatg
    22681 gagcacgctt cagcaagtta gaggcgaccg ccagctcatg ctgacgatat gggccagcgc
    22741 ttattttatg ctgattggcg ggtttgccaa aattaatcta ataccctatg ggatggaagt
    22801 ctgcggctac agtgatatag acagcggctt tttgtttgtg atggcggctg tcggcatcgg
    22861 cattggctgc tggctggccg gaaagctcag cgggcgtacg gtggaaatgg ggattgtgcc
    22921 tttgggggca atcggtatgg cagcatcttc tatcggcttg ggaatggttt cccccgccgg
    22981 cggagcagtc ccaacgccta ttcttgagaa tccacggtgg agtgttttta ttctgacatt
    23041 tctgatgggc atcagctgcg gcctttatat tgtatcaatt cattcattta ttcagattcg
    23101 aacaccggta tccattcgcg ggcgggtgct ggcggcttcc agttttctgg gctgggtggg
    23161 tgttctgttg gcggccttgc tggtcaaatt tgtctgcgga aatctcggtg tgccgccgcg
    23221 atggatgttt cttgtgtggg ggattttgac ctttctatta gctgttgcgg cagttatgat
    23281 tatgcccgat ttcctgatac ggctgatcgt tgtgctcatt acacggctta tctatcgcat
    23341 acgggtcgtt ggcatggaaa atattccaat ggagggacct gcactgatcg tttccaacca
    23401 tgtgtcatgg gccgatgccg ttgttctgat ggctacccag cagcggcgta ttcgctttgt
    23461 gatggacaag acaatttaca gtaatccttg gctgggatgg gtgttccggc tggggcggat
    23521 gattccaatc agccaagagc ccaaacagat tatcagtgcc ataaggcagg cccgacaggc
    23581 gttggaggaa ggatatctgg tctgtatctt tgccgaaggg atgcttacgc ggacaggaat
    23641 gctgggacgg ttccatgccg gttttgagaa aattgtcaga gataccgatt atcctgttat
    23701 tccgacatat ctgggcggcc tgtggggcag tatcttaagc cattaccacg gacgattgat
    23761 gagtgcgtgg ccgcagcggc tgccgtgtcc ggtttgtgtc cattttggtc gcccgatgcc
    23821 gtctgagtcc tcggtgcgcg atatccgctg ggcgattgag gaattatctg tggattattt
    23881 cagcagcaga tattctaatc agcacagctt aggacagaca ctgatacaag ccgctcggaa
    23941 acgctggaat cagccgtgcc tatctgatac ggccggaaaa cggctgacat tcggcaggac
    24001 gcttattgcc gctgcagcgc tggctgataa aattgagact ctaaccgctg gccaaagaaa
    24061 tcttgggatt tttctgccgc cttctgttgc aggggctttg gccaattatg cggctgcgct
    24121 gctgaataaa acagttgtca acttgtccta tactgcctcc gacagcgacc gggcttgcat
    24181 ggtggaaacc gccgaaatta aaacggtact taccagccgg acatttttag ataaactcgg
    24241 cttcagggct gacacgctgc cgggcgtgct ttttatcgaa gacctgctgg cggctgtctc
    24301 agacggagat aaacgcaagg catttctgaa ggcgcgtttc tggcctgctg acaaactggc
    24361 acgaattccc cgaaatttca gcgccgatga gactgccgtg attctgtttt cctccggcag
    24421 ttcaggcagg ccgaaagggg tggaattaac ccattgtaat atccagtccg atattgagtg
    24481 tgctttgacg gtatttaaga tatttaaaag cgataaattg tgcggcgtgc tgccgttctt
    24541 tcattcattt ggtctaactt gtaccctgtg gctgcctttg attgcagggg taccggcttg
    24601 ttttgtgtcc aatccgctgg gtggaaaact cgtaggcgaa acgattgcgg ctgaaaaggc
    24661 aaccttgctt ttcgctacgc cgacgtttct gctcaattat ctgcgccact gccgtccgga
    24721 agatttcaaa actatccgtt ttattgtggc aggagccgag aagctcaaga ctaagctgat
    24781 agatgctttt gaggaaaaat tcggaattcg gccctgtgaa ggatatggag caacggaatg
    24841 ttcccctttg atcgcattca atatacccga tgttgaaatt gacggtgtac ggcaaatcgg
    24901 caccaaagaa ggaacagtgg ggcatacaat tcccggattg gctgtcaaag tattggatgt
    24961 tgaaacaggc cggcccgtcg aactgggaca acccggattg ctttttgtca aagggcccaa
    25021 tgtgatgaag ggctacttga agcagccgga aataacccgg caggtcctta aagacggctg
    25081 gtataatacc ggcgatattg tcagcatcga tgaagacgga ttcgtaacca ttcatgaccg
    25141 cctgagccga ttcagcaaaa tcggcggtga aatggtaccc catctgacca ttgaagaaac
    25201 ggcgatggaa aagctgggtt ttcatgagtc ggttttggcc gtaaccagtc ttccggatga
    25261 aaaaaaaggt gaacagcttg ttattttata tgataaacga aaagttgacg gcgataaact
    25321 atatgctgtt ctgacagaga gcagtctgcc gaaactgtat atccccaaac gggagaacct
    25381 catcggcatc gatgaaatcc cgcatcttgg ttccggcaag ctggatatga taaagcttcg
    25441 ccagacagct gaaaaatata ttcagatgca gaaatcagaa agctgaaaag atacagattt
    25501 tcttcttaaa aaataacttt aaagggcaaa aaatatggaa ttttttgctg tactgctggt
    25561 ttttctggga ataggattag ctgctgccgg accggttgca attattcttg ccattgttct
    25621 gtttaataaa atcagcagca ttcagcagcg gctgaatcga ttggagggta agactgaaca
    25681 cagaggtctg tcaatgccgg ctgtacccaa agaaccggtc tggagcggtc aggcagcagt
    25741 tgacaggact gcggcaaagc cggctgttga agaaccgata attccgccgg cacagccccc
    25801 gatgccagtt caaattgccg agataaacgt ctcggctcca gtgtctaatc agtttggtgt
    25861 taagccctca ctgccctatg cgcctgcgcc tcagccaaaa ccgacagaca ctggattggg
    25921 acggattagg ccggtgccga ccgcaggcaa gagcggctgg gaactgacaa tcggcacgac
    25981 gattgcgctg gccgtgggaa caattctggt gattgcagga gtgggatttt ttctcaaata
    26041 tgtctatgaa aagatgctct ttggacctgt aacccgggtt agtatggtag ccgtcggcgg
    26101 gattgcggcc attatcattg gcgagctgac ccgccggcgc ggttatgaaa ttgttgccaa
    26161 aggcattgca gcactgggtt ttgcactttt gtatgccgcc gttttcggcg gcagccgggt
    26221 ctatcatctt tttggtactg aatgggcatt tgctttgtcg atgattatta ccggctcggc
    26281 aatgacatat gctgttgttc taaatgaagt gtttattgcc ttcctttcat tgctgggcgg
    26341 ctatttgtcg ccggctataa tcacaaccgg tcgaaatctt cccatcccgc ttttcagcta
    26401 tattttagtg ctcagcggcg gggcactggg ctgtgcactg gttcgccgat ggagggctgt
    26461 caattggata gcaatggtcg gcacctattt gctttatacc gcatggtttg aaaagtttta
    26521 cacagctgcc cagatacaga cagccctgtt ctggttgggg gtatttggat gcatttatct
    26581 tgtgcagccg gttctttatg ctctgacccg gaaagttgca gctcggttag aggatgtgat
    26641 tctgattgtg gtgaatagtg ctgtggtatt ttattacctt tggcggatgc tctatgccga
    26701 ttatcagggg cagctgggat tggcagctgc cgcatggggc atgatgcatc tggtgcttct
    26761 ggtctttgcg atgctgcggt gcagagacga cggccggctg caagctgttt tgggggtgct
    26821 gggcagcgca tttatagcgg cggcaatccc gctgtatttt gatgctttgc agccgtcact
    26881 ggttggctgg tccgttgagg ctatcgttct tacttttgtc agtatccgct ataacagttt
    26941 atggtcaagg gggatggcat ttttgactgc ggcggttgcc tcagcgggtt tgttctatca
    27001 tctgccgctg catccagatg ctgattttcg tttggttttc aatgcaccct ttggaacatg
    27061 gatttgggtg tctgtgtcaa ttctgatatg ccacgcactc tggcgtgtta tggcgtccgg
    27121 tcaaaatgaa gcgggccagc ttctgacaca ggtatatttt gtctgggggc gcctgctgct
    27181 ggcaattggt gctgtgctgg aatggtatgc atactgtgat tggcatatcg aatttctcaa
    27241 acagggacag gcctattttt tgatgggcgt catgatagtg gcagcaattt tactgatagg
    27301 ttttgcaact cgcccaatct gcccccacgg cgattttgtc cgaatgactg ccgttttgac
    27361 tgctggggcc ggagcaattt ttaccgcgat ggctatgatg ggtgtctatt atgaccaatt
    27421 caggatgttt ttgaacattc cgtttgcaat ggcatgcgtg tttataggtt cgttttattt
    27481 cagcatccgg caaatccgga aagctcttcc ttctgaatca caccagcgtc aaatgcccga
    27541 tgcattgttt atgctgggct tgcttcttat ctttgcagtc atcagtgaac agttttatat
    27601 gtattggtac tgccgcaatc agtacggccc gacagtggtt gactggaaga tgcgggcgca
    27661 tttctcgata ttgatggcct ggatgttcta cggattggtc atgctggcgg cagggattca
    27721 ttttaataaa ttctccatcc gaatactggg agtaatcgca tcggggcttt cggcagccgg
    27781 actgttctgc caacttccgc tgcatgcaaa tggtgcattt acatttgtat ttaatatgcc
    27841 gtttgttgca tgggtcgttg tgtctgccgg attgctggct gggcacgtgc tatggcgatg
    27901 gatgaaagct gctgttttgg aggaaagcag gcttgcagcc cagatttatt atgccgccgg
    27961 tgttctgttg ctgacggccg gctgctggtt ggaatggcac gcacactgcc gctggcagat
    28021 agagccgtct actgctggac attcgaatgt gttgctgggc ggcatcctat tgagcggaat
    28081 aacgatgctg atccttctgg cccggccgct tcccccggtc ggggattttg ttcagcgcat
    28141 cgggatatta tctgctctgg gcggggcagt atttacggca attgcagc
//
LOCUS       DAOTBO010000026        27337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1440_length_27337_cov_5.535078, whole genome shotgun sequence.
ACCESSION   DAOTBO010000026 DAOTBO010000000
VERSION     DAOTBO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 27337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27337
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_27337_cov_5.535078"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2736)
                     /locus_tag="PLE88_04955"
     CDS             complement(<1..2736)
                     /locus_tag="PLE88_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75851.1"
                     /translation="MKTQNRQLHKGSALILVIVVTVLLAVVGVMFLMISRAGQMESAA
                     VIQSKDLDAAVQTVLSRIDDVLVADLFGTNPARPGIADAGNLNPDSDEPWDLAEHHRG
                     FNNGLGVDGIAGTWDDMIDPGPDGQLDPAQAPAANDDVWLPGLLDDYWLASLEPVWWH
                     NNNTPADPTDDIYVWPHISDLWGTIQGASDSLYYQRFDRGQHVYYPQTAARYWLDPDN
                     SNVPSQWSTAPGVWEAYKVSAYNVRAKIIRPKDRMKIILQGAAPNVSEWNNPASTCIY
                     GARADADGDGVADSRWVLVPGLTTSRGEPVFAAVRIIDNCAMLNLNAASCFNVKGYAD
                     PKKRSPFSDMWTYWHEDPVNTFTLKPWHDNQKDGSGRYLTEINYLPFLRGRDLNGVLF
                     DGGASGESGDRWYDLMAAKGLAKVWGGNIYPFAPAAAQAFLLNMEKPLAGRFFDIGDE
                     LELRNRYLITSMTEARFERKSPPDPGVLHFTLDAGGGTYGALRTPVDENTAFVSWKIK
                     VDPANFDKWDLAGNPLAPTNPLFRYDRRHLCTFYSFDRMFRRGGYPLLEADLAGIPAG
                     SRGRIRSLLIPAGPVSTDIASPAEILKGTSGKPYNTIETRRRILQLLYAMREYFFQQN
                     GGDLHEAALSAAQFAANVIDFSDDGSPNSQVPGESEGPFYHPDYGAQANVDCTFITED
                     IIDKMLAEVTGGLVPAGAMPFGLETDDIVFGYERQPFISEVYAKRNVTTGVLEEFAIE
                     LLNPYPDDLKLARIDTAYTVNEDAWRIKVGDGAVINAAVGDLYKFVPKYDDQTAQSPG
                     RYVIRSSAAVTLASGPLASLTYELASLLQINTVTDKEIQLLRPAPAWIKNNRGIDYIV
                     VDKVSGDTVASMLLLVGQNAVKRQDTEWKFIYGRYALQQTGGPSPNYTHTLGQANS"
     gene            complement(2870..4054)
                     /locus_tag="PLE88_04960"
     CDS             complement(2870..4054)
                     /locus_tag="PLE88_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75852.1"
                     /translation="MDCKFDNMTVKSARGFTIAELLVAMALLVMLLTLSGLVFSTTVQ
                     AHRQAGASIEVSRNLRAVAEQINADFRGLRKEAPLFLWFNAVDTNGDAVPDTYYDAIH
                     FFADGDFQAAQTPLPNKMIYGNAARIYYGHANSVNLIANPIAVPDFKQAEILARRCHI
                     LTADSQLLTVMPYPNLGIPPDYSIFINPAASQFLLGNENVWEYDTISLTDWINTLNYS
                     TNNAGYFLRTVMFDTSVPDGNISRPMVNIDAADGLWNLFCQGVVQMQIQWAYQNEDLV
                     SFTGLAPAPPLFIGVRWWPDVDPVGNGNVPPNDVDSDFSLMGVNKFGVCFTLPKGTTD
                     TIANWYMVNTDNASKRCQTLAYPFRKDFYPKALKFTFVLKDSNGIFADGKIFTHIVYI
                     GD"
     gene            complement(4100..4984)
                     /locus_tag="PLE88_04965"
     CDS             complement(4100..4984)
                     /locus_tag="PLE88_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75853.1"
                     /translation="MTEMLIATGIMAIGLVMVATIFPVGVKLTTLTTERAVGAVAADE
                     AFAKIRLYGLRDFQFWPSALLHQADPAGFPDEYSTCSNYIDVNRYSLGPDRIYNTVDD
                     IPVTADWEQFRYPSVLPTAYPEGQKYHWSALCRRAGDKAVQITVFVNRRNTAGNSYYG
                     HLGFSGDASNPYPAANNLPWPSPVRVQVRLAGLRKLQVVPEDNPKWTNLLLGSSILDF
                     FSDGYTIADDYSGRIYRILEVNRTDGILTLAEDWITDPAKPAGLAHYIWVVPPAAGSD
                     RYPCVGVYQKVIQLDQIQ"
     gene            complement(5001..5855)
                     /locus_tag="PLE88_04970"
     CDS             complement(5001..5855)
                     /locus_tag="PLE88_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM75854.1"
                     /translation="MPTTRRQNGISLTEILVVAGIIAILMGISIPAARSLMDSYESTA
                     GVRKLINSAMSNARAIAVREQTYAGIRFQPDMNGHTYMIFIVHDPAATGLANGFRALT
                     GRRPMKLPDDVGAVCSYWIKRADFYTTYDWNKLMPVKLDDAVLTDNAANLAPVGTGGV
                     NRCLLDASTFSIIFSPQGRLTVHPVWVRNKDGTTDGSSNDTIFNVLVKVQAGISQFLQ
                     DDYGLDPTNCGVGPEISVQSFKLYSKSDYKQANAAARWTSYLQTINDEHISPYTGELV
                     LKYREQNP"
     gene            complement(5895..6764)
                     /locus_tag="PLE88_04975"
     CDS             complement(5895..6764)
                     /locus_tag="PLE88_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75855.1"
                     /translation="MKYKSKTLLTGFTLVELLTTLAVIGVLLALLIPALNQVTKTAAN
                     VKQRGQFHAIGVALEAFRSDTGDYPPSQWNPAVYGNYTASQRLAEAIIGQDGFGFHPA
                     SQFRADGTVDGTPATALYAPAVDVTANPDNLKLRKGPYLELENANAVKLGSLYGTAAA
                     PLVDTYILADMYKITKNLATGKMTGMPILYYKANRLKSRHAFEAADNETTIAQLNTYN
                     VYDSINGIAAKPNAAYHPLYANPGWFYGQTANPNFTSPPRPYRAESFLLHSAGPDGRY
                     GTSDDVFNFEQEK"
     gene            complement(6792..7754)
                     /locus_tag="PLE88_04980"
     CDS             complement(6792..7754)
                     /locus_tag="PLE88_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75856.1"
                     /translation="MKKLQNNSGFTLIELLTVVAIIAMLIALFGAGLQKVKIVQRNLQ
                     QKAALKAMEVGLELFSKDFQEYPDSAQVSDGSTVVCGAQRLAEALVGRDELGFHPKTK
                     WHPNLDMAAPAPHPGLSLYTSQTQKDRKSPYMEMRRLGVYTIYELWAGNNGGSTIYDS
                     GAVTPNGTHRAPVITDIFNRNRITLPSGDSERAGMPVLYFKADSSKRFRVDASNQPVT
                     NPAESEYKNWVYNFSDNLPMLRLPWLRDPAADTNNDIPPHYPDEDNDGTADNPAQVFY
                     EQLSRRHTPGSNWDKPFNPSTFVLISAGWDGIYGTKDDITNFNY"
     gene            complement(7870..8469)
                     /locus_tag="PLE88_04985"
     CDS             complement(7870..8469)
                     /locus_tag="PLE88_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75857.1"
                     /translation="MRQACRQHGFTLLEMLVTAAVILILISILIGAGRRIKERAAEDL
                     NRSMLEVLCTAVEQYYDEAGRFPFVANAGFNENDLEGLLAAVVDPPDSLVEKDGLGNE
                     ISNASSAALYYFLSRIPSSRSIAAAVTDTLITSKDASGQAIRMEQPAGSGIWTDLPRY
                     IDSWKTSIRYEYQLGWAFPVLTSAGPDKVFGTPDDITSK"
     gene            complement(8509..9465)
                     /locus_tag="PLE88_04990"
     CDS             complement(8509..9465)
                     /locus_tag="PLE88_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM75858.1"
                     /translation="MKRTKGFTIVELLTVMGVIAVLIGLLVPALGLVKDYSKRIQQKA
                     QMHSIDVGIEMFKNEFGNYPESKDNSFAAVNPIDSTVYCGANKLAEAMIGWDFLGYHP
                     RSGFTANGYNDVNGDGTAEFVYDTTNGIQTGVPGSPYNEVNGAENIANRKRFIDLENA
                     NGFQMQDVYAPAVVTGAGWTKSTNVVLCDVYAKKRLSSKKTGMPILYYRARTGFQFQD
                     YTNGIDDDIYNYNDNINLLALNSPDDNAIQHPLYDGSNDYADFESIILNQQVLQATVT
                     AANPTGVKRPYRADSFFLLSAGKDGLYGTADDVVNFEKEVTE"
     gene            complement(9527..10783)
                     /locus_tag="PLE88_04995"
     CDS             complement(9527..10783)
                     /locus_tag="PLE88_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM75859.1"
                     /translation="MAIFQYQALDAKGNETKGEIEALSSKEAISKIRNKGLFPTKVRA
                     QNVSKKAKVAREAAGPKRRGANGKVKIKAVTQFARQMSTLQDAGLAILRSLRILEEQQ
                     KKGTFKRVIGYVADEIEGGATLSEAMSHYPRCFNRLFVNMVAAGEVGGVLDVILNRVA
                     DFMEKSERLKSKIKGAMVYPAVVMTAAFFIVMGLMIFIVPTFSEVLKDMSDGKAQLPG
                     LTRGLLAISNWLLGRKGLNAALVAVCPFVVFSLLKLVRQFRYGRYAMDWLKLHTPIIK
                     KLVYKTSVARWTRTLATLISAGVPILEALNITRETSENEIYSAMLAKVQAAIRQGDTF
                     ANPLRQSKTVDSIVVNMVDVGEETGDLDKMLLKIADNFDEEADVLVGSLMSMLEPIMI
                     IMLGGLVGTIVLAMFLPMVKMIQVLM"
     gene            complement(10830..12581)
                     /gene="gspE"
                     /locus_tag="PLE88_05000"
     CDS             complement(10830..12581)
                     /gene="gspE"
                     /locus_tag="PLE88_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOM75860.1"
                     /translation="MAKIPPVYELKGRTLGRILIKMGLLTREKVHKCLEIQKQGGNKQ
                     KLGQIFIEQGLIKPSDLRIALAGQRGMEYIDIEGMEIPKAVLEQIPAQMARTYKIIPI
                     EFDKSRNQLTVAMDSPDNFRATDDLSTLMGYKVVAKVADEEVIRKYLDKYYAEEDSSI
                     NELIGEIESDKTLTQLAGRDASIDLDDLKDAAESTPVIKLLNLVLMQAIRDKASDIHF
                     EPFEDTYKMRYRIDGVLYEMEPPPKYIAMALSSRIKVMANLDIAERRLPQDGRIPLVM
                     GGNPVDLRVSILPTMFGESVVLRVLDRSQVNLSLDMLGMREQDLAKMRSLIQKPNGII
                     IVTGPTGSGKTTTLYSALKELNDVESKIITTENPVEYDIDGLIQVQIRPEIGLTFARC
                     LRSILRQDPDIVMVGEIRDLETAQIAIQASLTGHLVFTTVHTNDAPSTVARLLDLGLE
                     PFLMTATIEGIVAQRLVRKICPHCKTEYEPTEEMLMELNLRPEDVGGKRFFYGKGCDK
                     CNRSGYRGRLGIFEIMVFNDELRDLVMNNASTSVLREAARRAGMKTLRENGLAAIYDG
                     ITTIDEVSRETIAAEED"
     gene            complement(12875..14017)
                     /locus_tag="PLE88_05005"
     CDS             complement(12875..14017)
                     /locus_tag="PLE88_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOM75861.1"
                     /translation="MASLNIDRLLEACIKMGGSDVHITVGRPPVLRISGRLRSLDTKV
                     LEPEDTVALMKSITPERNQQELQEVGSTDYGFAFGDAGRFRTAVFKQRGNIAMVLRLI
                     PSQLLSLEQIGLPHICAALCRRPRGLFLVTGPTGSGKTTTLAAMINYINETLDRHIIT
                     VEEPIEYYHQHKKSIINQREVGLDVTTFAEALRRSLRQDPDVILVGELRDLETMEAAI
                     TAAETGHLVFGTLHTTGCQGTINRIIDAFPVSQQEQIRVQLSTNLIAVLSQTLCPKKT
                     GRGRVAAYEFMIVTPAISNLIRENKVYRIESSIQIGKKLGMQLLDDHLWELYDKEIID
                     LDELLDKARNPGEMLKAAKEKAGGSLGSAAAKKLEEEYGPVLKTSD"
     gene            complement(14054..15790)
                     /locus_tag="PLE88_05010"
     CDS             complement(14054..15790)
                     /locus_tag="PLE88_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOM75862.1"
                     /translation="MSKERRHLGEILYKKGYVSKENLIAAIKKGKQTNKRLGEVLLEM
                     KLATEDQIYECLAKQFGYEFVNLDNVQIPPNVAKLVPEDIIKRHKIIPIEVNNGELKV
                     VIKDPMDLDTLDLLRFRLNMEIRCCLASPTKIENFLNKSEADEPAKDDYRSSIDQTAA
                     ELAAMGHTIEAEVRRAQAAEDEMDDGPIVRLANLIIDEAVRMRASDIHIEPMADRVRI
                     RYRIDGVCVERDNIPKNMQAPLLARIKIMSGMDIGERRLPQDGRIKRTVDGQDIDFRV
                     SCLPGYHGESTVLRILRPESVNIGIQAIGFEPDDYERFIRIIRRPNGIFLVTGPTGSG
                     KTTTLYSALQELNRPDKKIITAEDPVEYNISGINQCQVKTEIGLTFEAILRSMLRQAP
                     NIILIGEIRDGVVADIAIQAALTGHLVFSTLHTNDAPSAITRLIDMGVKPFLVASSIQ
                     AIMAQRLVRVICKECKVVDTTPDPHYLRLLRITPEDIHKHPIYKGAGCARCQGTGYKG
                     RIAIFEMLEMNNQLRELAFSRAPASELRKAALASGMRSLLEDGKIKVFKGITTPDEVS
                     RVAQAEELVVDV"
     gene            complement(15863..17104)
                     /locus_tag="PLE88_05015"
     CDS             complement(15863..17104)
                     /locus_tag="PLE88_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOM75863.1"
                     /translation="MKKVGIISIGNELLNGHTTDTNASWLAGELLAMGLPTAGVWTVP
                     DEISRIIHAFDEASREAEILLITGGLGPTDDDLTRQAAASWLGTDLVFQPEWLDKIAD
                     FFKKRGRTMPARNQSQAYIPAGCRRLDNPAGTACGFWGLRNGVFFAAMPGVPEEMKRM
                     FNEQVKDQIDTAAQGAFTAAGKLHCFGAGESDIAQRLGDLMNRRRNPLINCTCGAGEV
                     VLHIIASADSKMQAMKMVQDDKQRLKALLGNIVFGEDDSSLPAAVGQLLREKHKTIAA
                     AESCTGGLLSKMLTDIPGSSAYMLAGWVTYSNEAKVRQLGVPEQFIRDFGAVSRQVAS
                     AMAEGAAKRSGADIAVSITGIAGPDGGSEEKPVGLVFIGVWTDGICDVQRCRFGDADR
                     QSIRQRAAMTALNLVRLRLQV"
     gene            complement(17260..19569)
                     /locus_tag="PLE88_05020"
     CDS             complement(17260..19569)
                     /locus_tag="PLE88_05020"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOM75864.1"
                     /translation="MDNAIQRTINMMTGPASSRPPMTPPQGPARPAGGAGGGMELTPR
                     EILGILRRHLWMIILMTILGTVIGGGLWYLCDRYYPRYTSVRAIDVDPPVDMDPMQIA
                     SIQVQKDLYYAFRFTKASMIQQQNMLLTLLEQPRVRETDWFKSFAKIDSQGQIVGDVE
                     KAIREALKDLQDNLSATAPRDNNYLLVSMKCRNARDAKTIVDEMVRIFMRQQQELAQS
                     TLKQELAQLKNQRDQIQTKLNQIEASLESIRSGTRFARLNLGENQSFRDYMDEKLADL
                     ENRYSEYDSEKSRLESIIATLDVRAKAYDFDEVVRQEVENDPIARQMRSNIAAMEPLL
                     DQQRARFGDEHRLVKQTQAALKKMYDEYDARQKMIGELIRQSNLINARDTMTALTSQL
                     ETATQQLNAARAEYKEIDRIRAEYARYEQQREENQKLLEDMNTLIEKKNAQHDNPRLS
                     KLSSPYGATDPREKSFPRWELFFPGGFILGLLAGLGLAFLIELANDLVRTPSDVVRHL
                     RVPLMGMIWHVSDDDEAEGVDLYHVVRQAPYSMMSEGYRQLRTNLRLLGPGGAMHKVL
                     CVTSGQAGDGKTTVAVNLASTLLMEGRRVLLLDANFRRPSTARLFPRSQANGSSGFSD
                     FGLSNYLMGQCSEDRIIRDSGITGLYLIDSGPLPANPAELFTSERMKTLLDYCRSQFD
                     YIIIDGPAMLVSDAKTLAAQADAALVVLNAAVTRRGAALRILRELKDINANVAGAVLM
                     GAKSRKGGYFREVYRSYQKYQQVQVQQTV"
     gene            complement(19599..24326)
                     /locus_tag="PLE88_05025"
     CDS             complement(19599..24326)
                     /locus_tag="PLE88_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75865.1"
                     /translation="MAKKLNKKVALVVGILLVGIVCVLAGGVVYLKVHRDPDRALAQA
                     RQALEAGDYKKAESQLGRAFAYGKDDAYKIERLFELADFHLIHNAQHQAEWNKAMGCW
                     SKIVSIDTNNVQARRKLLDFYYQAAEAGDARAWQNVLENTTKLLDTLKAGQAEPDTTL
                     LTAHAKSLLYIAQRGETTDRRQHLSDCMTILEQLKEKEPGNAEVYLLLAEAAGLQGEL
                     DTQSGVIRAAENARQKALEHLQAGVERADDKSAAVANLLRYRLQSTVGDPNGLEAFRA
                     EIETYRTQIQPNDVFWLVVSIAYEFPGRQSAQAEINQAIEAVRRAHEMKPDNFEYVLR
                     LARLMYRKANAFADAAALADARQIAEDALSMDAVQDVTGPLQGRNLNYRFTLNAFLAD
                     LYLDHALTARREGRTAEADELTAKAESCVAVISGILGTSDSPTAQKYQGLLALAKDQK
                     DKGLRLLHKAYEQWRALDRPQEFSNADPLVCAVLADLMKDSGEPGMRREFLQWAIYNN
                     SRYVLQKPKLILDYAEVLGQLRAWNNTERMVEEYQNRYGANGQSQMLAIRAAAALGKF
                     DQARRLLAQLDGTSAPVLQLRLEVLTAQIAQIAGSIAEGRQAQPPKEPTAEQLKKLDT
                     LRAERNTVLQQALQLEPAQMDPQLLNVICMDMIQNKQAPQAAAFLDIYLAANPDAYGL
                     QLLRLRASQDDPAAQPTAQLQEQAADRIQNPRQKALLRSALYRAQGDYEKAMAALMQD
                     AGPDAANDPEILNAQFDIAVDQKDSAKAEGLLQMIRSKNADGCEGGLAAARVEMLKEN
                     YPQALRRLDECLTLRPLSSYLYYLKSQVQHQMQDSTAAIQNARQAVQMNPQHAAYARN
                     LAMLLFARNNELGTKVTPEQRSEAEQALTLAMLLNPADWQLQGLYAESIQQQAPDRAL
                     QIRQRLLQTVPTASNALMLGNMALRMAQSEWDTAKRTGLVELAGKAYQQAIDLEPDND
                     SIRQAYADYLRLTQQSDKALDLLKGDQNLLWKYYLRGGQFEQAEAILKDLLAKHPDDV
                     LLYQGLVIASEGAGKRQQVKEYLDALVKLEQTKDGELWIMQKYLDNAFTAEAESRIPS
                     FQERYPDDITILLIKAWTAMGHGRLDEALSLTNLYLEKDTANAGAWRLRGRLYRLMNQ
                     QQKAIEDLQRSKGLQDNPMVRMELANVYNETRQITAAIGELASALDDPKAPIQVRLML
                     EALYQKNNRAADLDKFYQGTLEKYPNSAFWHYRAGMYYLSQKNYTRAEELLLSAWKLA
                     SAQNQPNLELVQGYLEVLYAAKQYDKLITFAGGLIDTPGAPVAYMYLGLVQSQQGQKD
                     KAIDSFYKALDKAQTSETMQELILQKVRPAAGEEVLDGWVTKTLAADAKSLPALLLAC
                     RLAQEKSAYNKAIEYADQCIGLIAPDNPQLFLYNLKKVNLLIMAYAKTADSSYFERAA
                     DMLEKMLQIQPNNSSLLNNMAYLLADNDQQLEKALNYARSAHQQDPGNPVFLDTYAFV
                     QCKVGQYDTAEQNLLRAIQIYDVSGQPVPWDVYKHLAMAQEGLNKPQQAMETYQKALD
                     SAQEMPDKEKQQLQKAIERLRQQ"
     gene            complement(24357..25148)
                     /locus_tag="PLE88_05030"
     CDS             complement(24357..25148)
                     /locus_tag="PLE88_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75866.1"
                     /translation="MASSALQSYLKPSFVVCTALLLTAAAAKETIIRVLGVQMTKQRI
                     ELRHPLAEMNEQALRPFVVKQKEQILNRDVLESLGTEEYLQWILEDPEAPAGSPLRFC
                     SLFITYYTGNPDMVPHVPDECYVGGGNERKDGQTLTLDIPLPQGERKLAFQYVIFGRA
                     GTMAAKEDNFSVQYFFHANGRYCGDRTQTRLVLGSNWFSKYSYFCKVEWKFYGADAFG
                     VVYPSQEQTLEASRRLLARLLPELEANHWPDWEAANAKPQQRQRQ"
     gene            complement(25149..26132)
                     /locus_tag="PLE88_05035"
     CDS             complement(25149..26132)
                     /locus_tag="PLE88_05035"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOM75867.1"
                     /translation="MNQKQDILVAADRTEKQPSWLDLGIHLYIRAAIIALLIWWIFRE
                     EINGLVLQWISNPSWSHGFLIPLFSLYFLHQNKEEILAIQQVRPSWFAGLGALLFLLA
                     LHPINLVMLKFMYGKPLTMIAVIGAVVLFLGGWKLLRYTWLPVAFLFFAVPLPQRLYF
                     QLTNPMRQLAAQAATVILNLVPQLEAEVHGSVIDVLYKGKALEPALDVADACSGMRLL
                     MAFVALGVAMAYLHWRPIWQRLVLLASTVPIAILCNIVRVTVTGFIYVLGNPKYAQGI
                     YHDMLGMLMLPLAFALYGGLAWLMSNLFVEEEQTQEDIIVRKSVPRQSQET"
     gene            complement(26152..>27337)
                     /locus_tag="PLE88_05040"
     CDS             complement(26152..>27337)
                     /locus_tag="PLE88_05040"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOM75868.1"
                     /translation="AAGLTEAQLEREIASILSPSILKNPSVTVTLMASQQRYFSIIGQ
                     GVAQSARYPLPRYPIRLTDAIALAGGMGDFNVSYIYVSRDVRQQDVEPGAAGTDSKPQ
                     QPAPGKEDEMLELITPSETRRPGGLFVTSAEMATQGELEALAAPEGIEPVQGVTDVKS
                     PEALLPQQTSKRIEWVFEDGKWKPIEVEGPSEQSPQQPELLRPEQYAQTKPQRQIPSD
                     FGLSEVGRSSTVTRVIQIPRDRLESGDPRYNIVIRPGDRITVPRDLVGEFWVAGNVNA
                     PNAYSITGRPITLKQAIATAGGLNAIAWPQKVEVVRRIGKNKAGLMQEEIVLVDLQKI
                     AQGLQPDFFIKPYDYINVGTHGTSRWLAVLRNAFRATYGFGFIYDRNFADRDYLGDPL
                     PF"
BASE COUNT         6294 a         7913 c         6566 g         6564 t
ORIGIN      
        1 gctgttggcc tgcccaagtg tgtgcgtgta gttgggagaa ggaccacctg tctgctgcaa
       61 tgcataacgc ccataaatga atttccactc cgtatcctgc cgtttgacag cattttgccc
      121 cacaagcagc agcatagaag caaccgtgtc gccagaaacc ttgtccacaa ctatataatc
      181 gattccgcgg ttgtttttaa tccacgccgg cgccggccgg agcagctgaa tttccttatc
      241 ggttacagta tttatctgaa gaagggatgc cagttcatag gtaagcgatg ccagcggacc
      301 ggaagccagc gtcaccgcag cagaacttcg aatgacatac cggccgggtg attgggcagt
      361 ttggtcatca tacttaggta caaatttata gagatccccg acagcagcat taatcaccgc
      421 cccatcgcct acttttatcc gccatgcatc ttcattaaca gtataagccg tatcaatacg
      481 tgcaagcttc agatcgtcag gataaggatt cagcaattca atggcaaact cttccagtac
      541 gcccgtagtc acattgcgtt tggcatacac ttctgaaata aagggctggc gctcatagcc
      601 gaaaacaata tcgtctgttt ccagaccaaa cggcatagct ccggccggca caaggccgcc
      661 ggttacctct gccagcatct tgtcaatgat gtcttctgta ataaaggtgc agtccacatt
      721 ggcctgcgcg ccgtaatcgg gatggtaaaa aggcccttcc gactccccgg gaacttgact
      781 gttgggcgat ccatcatccg aaaaatcaat cacattcgct gcaaattggg cagcgcttaa
      841 ggccgcctcg tgaaggtcgc cgccgttttg ctgaaagaaa tactcccgca tggcataaag
      901 cagctgcagg attcggcgcc gcgtttcaat agtattgtac ggcttgccgg atgtgccctt
      961 gagaatctca gccggcgaag cgatgtctgt ggaaacagga ccggctggaa tcagcagcga
     1021 gcgaatccgg ccgcggcttc cggcaggaat ccccgccaaa tctgcttcca gcagcggata
     1081 gccgcctctt cggaacatcc ggtcaaagct gtaaaatgtg cacagatgcc gccggtcata
     1141 tcgaaacaaa ggattcgttg gcgcaagcgg attgcccgcc aaatcccatt tatcgaaatt
     1201 ggccggatcg actttgattt tccatgatac aaatgccgta ttctcatcga ccggagttcg
     1261 caacgcccca tatgtcccgc cgccggcatc caatgtaaaa tgcaaaacac ccgggtcggg
     1321 aggcgatttt cgttcaaacc gcgcctctgt cattgaggta ataagatagc ggttccgcaa
     1381 ttccagttca tcaccgatgt caaaaaatcg gccggcaagg ggtttttcca tattcagcag
     1441 aaacgcctga gcagccgcag gagcaaaagg ataaatattg cccccccata ctttggctaa
     1501 tccttttgct gccattaaat cataccaccg gtcgccggat tcccccgaag ctccgccgtc
     1561 aaacaaaacc ccgttcaaat cccgcccgcg cagaaacggc agatagttta tttcagttaa
     1621 ataccgccct gaaccgtctt tttgattgtc atgccatggc ttcaaagtaa acgtattgac
     1681 aggatcttca tgccagtaag tccacatatc cgaaaacggc gatcgtttct tagggtcggc
     1741 ataacccttc acattgaaac agctggccgc attcaggttg agcatcgcgc agttgtcgat
     1801 aatgcggacg gcggcaaaga ccggctcgcc ccggctggtc gtcaggcccg gcaccagtac
     1861 ccagcggctg tcggccacgc cgtcgccatc ggcatcggct cgggcgccgt aaatgcaggt
     1921 actggccgga ttgttccact ccgacacatt cggcgctgct ccctgcagaa taatcttcat
     1981 tctatccttg ggtctaatta tttttgcccg cacattatag gcactgacct tataggcttc
     2041 ccaaacccct ggagcagtgc tccactgcga tggaacattg ctgttgtccg ggtcaagcca
     2101 atatcttgct gccgtctgcg ggtaatatac atgctgacct cggtcaaacc gctgataata
     2161 caagctgtct gaagccccct gaattgtccc ccacaaatcg ctgatatgag gccagacgta
     2221 aatatcatcc gtcgggtcag caggcgtatt gttattgtgc caccagaccg gctccagcga
     2281 ggccagccaa taatcatcca gcaatcccgg cagccagaca tcatcatttg ccgccggcgc
     2341 ttgggccgga tcgagctggc cgtcaggacc gggatcaatc atatcatccc acgtaccagc
     2401 tattccatcc acgcccaaac cgttgttgaa gccccgatga tgttccgcca aatcccaggg
     2461 ttcatcggaa tcgggattaa gattgccggc gtcggcaata cccggccggg ccggattggt
     2521 gccgaacaaa tcggcaacaa gaacgtcatc gatacgggat agcaccgtct ggacggcagc
     2581 atccagatcc ttgctctgga ttactgcggc cgattccatc tggccggcac gggagatcat
     2641 caggaacatt acccccacca cggccagcag caccgtaacg actatcacca gaatcagagc
     2701 ggaaccttta tgcagttgtc tattctgtgt tttcacaata ccacctttat tgtcatttta
     2761 gactatacag atactcctgc tggaaaaatc cctttgcaga acgccttgtt tgccttcaat
     2821 gacatttcca ttccgtttca gcttaccgtc aatggccccg cgtttgtctt caatcgccta
     2881 tatatacaat atgcgtaaat atcttgccgt cggcgaaaat cccgttgctg tccttgagca
     2941 caaacgtaaa cttcagcgct ttgggataaa aatccttccg aaaaggatat gctaaagttt
     3001 ggcatctttt gcttgcatta tccgtattta ccatatacca attagcgatg gtatccgtcg
     3061 tccctttggg cagcgtaaaa cacacgccga atttattgac gcccattaac gaaaaatcgg
     3121 aatccacatc attggggggc acattaccat ttcccacagg gtccacatcc ggccaccatc
     3181 gcacgcctat aaataaaggc ggagcggggg caagccccgt aaaggagaca agatcctcat
     3241 tctgataagc ccactgaatc tgcatctgaa caacaccttg gcaaaagaga ttccacagac
     3301 cgtcggcggc gtcgatattg accatcgggc ggctgatatt gccgtccggc acagaggtat
     3361 caaacatcac cgttcggaga aaataaccag cgttattggt gctgtaattg agagtattga
     3421 tccagtccgt cagcgaaatc gtatcatact cccagacatt ttcattgccc aaaaggaact
     3481 gggatgctgc gggatttatg aaaatagaat agtcgggcgg tatgcccaaa ttgggatacg
     3541 gcataaccgt caaaagctga ctgtcagccg tcaaaatgtg gcagcggcgg gcaagaatct
     3601 ctgcttgttt aaaatcagga acagctatcg ggttcgctat cagattaacc gaattggcat
     3661 ggccgtaata aatgcgggcg gcattgccgt aaatcatctt gttcggcagc ggcgtttggg
     3721 ccgcctgaaa atccccatcg gcaaaaaaat gaatggcatc ataataggta tccggaacag
     3781 catcgccatt ggtatcaaca gcattaaacc acagaaacag cggcgcttct ttccgcagac
     3841 cgcgaaaatc ggcattgatc tgctcggcaa cagcccgcaa attgcgggat acctcgatcg
     3901 atgctcctgc ctgccggtgg gcctggactg tcgtcgaaaa gaccagcccc gacagggtca
     3961 gcagcatcac cagcagagcc attgccacca gcagctctgc aatcgtaaag ccccgtgctg
     4021 attttaccgt catattgtca aatttacaat ccattttcga aaccgctggt tgcacagatg
     4081 tatttctcaa ctgccgctgt tattgaatct gatccaactg aatcactttc tgatacaccc
     4141 cgacacaggg atatcggtcg ctgcccgccg ccggtggtac aacccagata taatgggcca
     4201 atcccgccgg ctttgccgga tcggtaatcc agtcttccgc aagggttagt attccatcgg
     4261 tgcggttcac ttcaaggatt cgataaatcc ggccggaata atcatctgca atcgtatagc
     4321 cgtcactgaa aaaatccaga atgctgctgc caagcagcag attggtccat ttcggattgt
     4381 cctccggtac aacctgcagc ttccgcaaac cggcaagacg cacctgcact ctgaccggcg
     4441 aaggccaagg gagattgttc gccgccggat agggattgga tgcatcgccc gaaaatccca
     4501 aatgcccgta atagctgttt ccggccgtat tgcggcggtt gacaaacacg gtaatctgca
     4561 ccgctttgtc tcctgcccga cggcacaggg cagaccagtg atacttctgc ccttcgggat
     4621 aagcagtcgg caaaacagac ggatacctga actgctccca atcggcggtt acaggaatat
     4681 cgtctacggt gttatagatt ctatccggcc ccagactata ccggttgaca tcaatataat
     4741 tgctgcaggt actgtattca tccggaaaac cggcaggatc tgcctgatgc agcagtgcgg
     4801 acggccagaa ctggaagtcc cgcaggccat aaagccggat tttggcaaag gcctcatccg
     4861 ccgcaaccgc ccctactgcc cgttcggtag tcagtgttgt cagtttgacc ccaaccggaa
     4921 agattgtggc taccatcacc aatccgattg ccataatgcc ggtggcaatc agcatctcgg
     4981 tcaatgaaaa tccaaattgt ttacggattc tgttcacggt atttgagcac cagttctccc
     5041 gtataagggc tgatatgttc gtcattgatg gtttgcaaat aacttgtcca gcgggcagcc
     5101 gcatttgcct gcttgtaatc tgatttgctg tacagcttaa aggattgcac gctgatttcc
     5161 ggaccaacac cgcagttggt ggggtcaagt ccataatcat cctgaagaaa ctgactgatt
     5221 cctgcttgga cttttaccag cacattaaaa attgtatcat tgctgctgcc gtcagttgtg
     5281 ccgtctttat ttcgcaccca aaccggatga acagtcaggc ggccttgcgg cgaaaaaata
     5341 atcgaaaagg tgctggcatc cagcagacac cgatttaccc ctcctgtgcc gacaggggca
     5401 agattggctg cgttatctgt taaaactgca tcatcaagtt tgacgggcat cagcttattc
     5461 cagtcatagg ttgtgtagaa atccgcccgc ttaatccagt acgaacagac cgcccctaca
     5521 tcatccggca gcttcatcgg cctgcggccg gttagtgccc gaaatccgtt ggcaagcccg
     5581 gtcgctgcag ggtcgtggac aataaaaatc atataggtat gcccattcat atccggctga
     5641 aagcgaatgc cggcataggt ctgctcgcga acagcaatag cccgggcatt ggacatggcg
     5701 gagttaatca gtttgcgaac accggctgtc gattcgtacg agtccattaa gctgcgggct
     5761 gccggaatgg agatgcccat caggatggcg ataattcccg ccacgacgag aatctcggtc
     5821 agtgaaatcc cattttgtcg tctggtcgtt ggcattctaa atcccagccg ctgcttggca
     5881 ctcgcagcga ggcgctattt ttcctgctca aagttaaaga catcatccga tgtgccatat
     5941 ctcccatcgg ggccggcact gtgcagcaga aatgattcag cccggtacgg ccgcggggga
     6001 ctggtaaaat tcggattggc tgtctgcccg taaaaccagc cgggattggc atacagcgga
     6061 tgataggccg cattcggctt ggcggctatg ccgttaatgc tgtcatagac attgtaggta
     6121 ttcaactggg ctattgtagt ttcattatcg gctgcctcaa aagcatgacg gcttttgagg
     6181 cgattggcct tgtaatacag aatcggcatt ccggtcattt tgcccgtagc cagattcttc
     6241 gtaattttat acatatcagc cagtatataa gtatccacca aaggagcggc cgctgttccg
     6301 tacagactgc ccagtttgac cgcattggca ttttccagct ccagatacgg ccctttgcgc
     6361 agtttaagat tgtccggatt ggccgtcaca tccaccgccg gagcatacag ggctgtggcc
     6421 ggcgtgccat caactgttcc atcggctcgg aactgcgacg caggatgaaa gccgaagccg
     6481 tcctgaccga taatggcttc tgccagccgc tgcgaggcgg tataattgcc gtacacagca
     6541 ggattccact ggctgggcgg atagtcgccg gtgtcgctgc ggaaggcctc caaagccacg
     6601 ccgattgcgt gaaactgccc tctctgcttg acattggcgg cggttttggt cacctgattg
     6661 agtgctggta tcagcaaagc cagcagcacg ccgataaccg ccagcgttgt cagcagctcc
     6721 accagcgtaa agccggtcag cagtgttttc gatttgtatt tcataaaaac gcctcctgaa
     6781 tgctaaccgt atcaatagtt aaaattggta atgtcatcct tggttccata aatgccgtcc
     6841 cagccggcag aaatcaggac aaaagtgctc ggattgaagg gcttgtccca attcgaaccc
     6901 ggtgtatgac gccgcgacag ctgttcgtaa aaaacctgcg ccggattatc cgcagtccca
     6961 tcgttgtcct catccggata atgcggggga atatcattat tcgtatctgc cgccgggtcg
     7021 cgcagccaag gaagccgcag catcggcaga ttgtccgaaa aattatagac ccagtttttg
     7081 tattcggatt cagcggggtt cgtaaccggc tggttagagg catctacccg aaaccgcttg
     7141 gaagaatcag ccttaaaata taataccggc attccggccc tctcactgtc gccgctgggc
     7201 agggttatcc ggttgcggtt gaatatgtcc gtaataaccg gcgcccgatg ggtcccattg
     7261 ggtgttaccg ccccggaatc gtaaatcgtc gaaccgccgt tattgcccgc ccataactca
     7321 taaatggtat aaacccccag acgcctcatt tccatatacg gcgatttccg gtctttttgg
     7381 gtctgggagg tataaagcga aaggcccgga tgaggtgccg gagccgccat gtccagattc
     7441 ggatgccatt tggttttggg atgaaatcca agctcatccc ggccgacaag ggcttcggct
     7501 aaccgctggg caccgcagac aacggtactt ccatcggata cttgagccga atccggatat
     7561 tcctgaaaat ccttgctgaa cagttccagt cccacttcca ttgccttcag ggcggccttc
     7621 tgctgcaggt tgcgctggac aatttttacc ttttgaaggc cggcaccgaa cagcgcaatc
     7681 agcatcgcaa ttatcgccac cactgtcagc agctcaatga gcgtaaaacc gctgttgttt
     7741 tgcagctttt tcattgtttc aagtcccggt ttaatatgtt cgggtttttt gaatttttaa
     7801 gcgataagca ttgagaagcc ccactaaacc aacggcttag tcggctccct aactcaaaac
     7861 ttaaaatgct tatttacttg taatatcatc gggcgtcccg aaaactttgt ccggccctgc
     7921 gctggtcaag accggaaaag cccagcccag ctggtactca tagcgaatgc tggtcttcca
     7981 ggagtcgata taccgcggca ggtcggtcca gatgccggac ccagccggct gctccatccg
     8041 aattgcctgc cccgaagcat ctttactggt tatcagcgta tccgtgacag cggcggcaat
     8101 cgagcggctg gacgggattc ggctcagaaa gtaatacagc gccgcactgg atgcattgga
     8161 aatctcattg cccagcccgt ctttttccac aagcgaatcc ggcggatcaa ccaccgcagc
     8221 caaaagccct tctaaatcgt tctcattaaa tccggcgttt gccacaaaag gaaaacggcc
     8281 ggcttcatcg tagtactgct cgacggctgt gcagagaact tccagcatgc ttcggttaag
     8341 gtcttccgcc gcccgctcct tgatgcggcg gccggcgccg atcaatatgc tgataagaat
     8401 cagaataacc gccgccgtaa ccagcatttc cagcagcgta aaaccgtgtt gtcggcacgc
     8461 ttggcgcata tagacccttt agcgatgcaa gggaaagcat tcccccgctt attcggtaac
     8521 ttccttctca aagttgacca catcgtcagc cgttccgtaa agaccgtctt tgcccgccga
     8581 cagcagaaag aaggaatccg cccgataagg ccgcttgaca cccgtgggat tggcagccgt
     8641 tacagtggcc tgaagaacct gctgattcaa aatgatgctt tcaaaatctg cataatcgtt
     8701 gcttccgtca taaagcggat gctgtatcgc gttgtcatcc ggcgaattca gagccagcag
     8761 attgatgttg tcattatagt tataaatgtc atcgtcgatg ccgtttgtat agtcttgaaa
     8821 ctggaaaccc gtgcgggccc ggtaatacag aatcggcatc cctgtcttct tggaagacag
     8881 acgctttttg gcatacacat cgcagagcac aacattggtt gacttggtcc agccggcccc
     8941 tgtcacaaca gccggtgcat agacatcctg catttggaag ccgttggcat tttccaagtc
     9001 aatgaaccgc ttgcggttgg cgatgttttc ggcgccgttg acttcattat agggcgaccc
     9061 gggcacaccc gtctgaatgc cgttggttgt atcatagaca aactccgctg tcccgtcgcc
     9121 gttgacatca ttgtagccgt tggcggtaaa accggaacgg ggatgatacc ccagaaaatc
     9181 ccaccctatc atggcctcgg cgagtttatt ggctccgcaa tacacggttg aatcgatggg
     9241 atttacagcg gcaaaggagt tgtctttcga ttccggatag ttgccgaatt cattcttaaa
     9301 catttcaatc cccacgtcaa tactgtgcat ctgggccttc tgctggatgc gcttggaata
     9361 gtccttcaca aggcccagcg ccggcaccag cagtccaatc agaaccgcaa tcacccccat
     9421 cactgtcagc agttcaacaa tcgtaaaccc ctttgttcgc ttcattgttt tgtcctttct
     9481 aaaaataaat gagttcgttt tctgcagtcc ggatctgtcc gtccttctac atcagcacct
     9541 gaatcatttt taccatcggc agaaacattg ccagcacaat cgtcccgacc agtcccccca
     9601 gcataataat cataatcggc tccagcatgc tcatcagcga accgaccagc acatcggctt
     9661 cctcgtcaaa attgtcagca atcttcagca gcatcttatc gagatcgcct gtttcctcgc
     9721 ctacatcgac catgttgacc acaatggagt ccactgtttt cgactgccgg agcggattgg
     9781 caaatgtatc cccctgccgg atagcggcct gtaccttggc cagcatcgcc gaatagattt
     9841 cattttccga tgtttcacgg gtaatgttga gcgcttccag aataggcacg ccggcactga
     9901 tcagcgtcgc cagcgtccgc gtccagcggg caacggaggt tttatacacc agcttcttga
     9961 taatcggagt atgcagcttc agccagtcca tcgcatagcg cccatagcgg aactggcgaa
    10021 caagcttcag cagcgaaaac accacaaacg gacagaccgc cacgagtgcg gcattaagtc
    10081 ccttgcgccc cagcagccaa ttgctgatag ccagcaggcc gcgcgtcagc cccggcagct
    10141 gggctttgcc gtcgctcata tccttcagca cctctgaaaa agtcggaaca ataaaaatca
    10201 tcagccccat cacaataaaa aacgcagctg tcatcaccac cgccggatac accatggccc
    10261 ctttgatttt ggatttgagc cgttcgcttt tttccataaa gtcggccacg cggttgagga
    10321 tgacatccag cacgccccca acctctccgg ccgcaaccat gttcacaaac agccggttga
    10381 agcagcgcgg atagtggctc atcgcctccg acagggtcgc accgccttca atctcatccg
    10441 ccacataccc gatcacccgc ttgaaggtcc cttttttctg ctgctcttcc agaatccgca
    10501 gcgaccgcag aatcgccaga cctgcatcct gcagagtgga catctgccgg gcaaactgcg
    10561 taaccgcctt gattttgacc ttgccgttgg ccccccgccg cttcggacca gcggcctctc
    10621 tggctacctt ggcctttttg gacacattct gtgcgcggac tttggtgggg aacaatcctt
    10681 tgttgcgaat cttgctgatg gcttctttgc tgctgagcgc ctcaatctcg cctttcgttt
    10741 cgtttccttt ggcatccaat gcttgatatt gaaatatcgc cattgtcgtg ccctcctaag
    10801 aaaataaagc cgtataccga ttcatcgcat taatcctctt ccgccgcaat ggtttcacgg
    10861 gacacttcat caatcgtggt gatgccgtca tagatagctg ccagaccgtt ttcccgcagc
    10921 gttttcatcc ctgcccgcct tgctgcttcc cgcagaacgg atgtagaggc attgttcatc
    10981 accaaatccc gcagctcatc attaaacacc atgatttcaa aaatgcctaa tcgcccgcga
    11041 tagcccgacc ggttgcattt gtcgcagccc ttgccgtaga agaaccgttt gcccccgaca
    11101 tcttccggac gcaggttcag ttccatcagc atctcttcgg tgggttcata ttccgtcttg
    11161 caatgggggc atattttacg caccagccgc tgggccacaa tgccctcgat ggtagcggtc
    11221 atcaagaatg gttccagccc caaatccagc agacgggcaa ccgtgctggg ggcatcgttg
    11281 gtatgcacgg tggtgaaaac caaatgccct gtcagcgagg cctgaatagc aatctgtgca
    11341 gtctccaaat cgcggatctc accgaccatc acaatatccg ggtcctgacg cagaatggat
    11401 cgcagacagc gggcaaatgt cagaccgatt tcaggacgta tctgcacctg aatcaatccg
    11461 tcaatgtcat attcaaccgg gttttcggtc gtaatgatct tgctttcgac atcgttgagt
    11521 tctttgaggg cagaatagag cgtggtggtt ttgccggagc cggtcggacc tgttacaata
    11581 atgatgccgt tgggcttttg aatcaggctg cgcatcttgg ccaaatcctg ctcgcgcatc
    11641 cccagcatat ccagcgacag attcacctgc gaacggtcga gcacgcgaag caccacactt
    11701 tcgccaaaca tcgtcggaag gatgctcaca cgcagatcaa caggattgcc gcccatcacc
    11761 agcgggatac gaccgtcctg cggcagacgc cgctccgcaa tatccagatt ggccataacc
    11821 ttgatgcggc tgctcaacgc catagcgata tacttgggcg gcggctccat ctcgtagagc
    11881 acgccgtcaa tacgataccg cattttatag gtatcctcga acggttcaaa atggatatcc
    11941 gaggccttgt ctcgtatggc ctgcatcagc acaaggttca gcagctttat caccggcgtt
    12001 gattcagcgg catcttttaa atcatccaag tcaatgctcg catcgcggcc ggcaagctgg
    12061 gtaagggttt tatcgctttc tatttcgcct atcagctcat tgatgctgct gtcctcctcg
    12121 gcgtaatatt tgtccaagta ttttcgtata acctcctcat ctgccacttt ggctacaact
    12181 ttatagccca tcagcgtgct taaatcatcg gtagcccgaa aattgtcggg actgtccata
    12241 gcaacagtca gctgatttcg agacttgtca aactcaatgg gaatgatttt gtatgtccgc
    12301 gccatttggg caggaatttg ttccaacact gctttgggta tttccatccc ctcaatgtct
    12361 atatattcca tccccctctg gccggcaagt gctatccgta gatcactggg ttttattaag
    12421 ccctgttcaa taaagatttg acccagtttc tgtttatttc ccccctgctt ttgaatttca
    12481 agacatttat gtaccttttc tcttgtcaac agacccatct taataaggat gcgtccaagt
    12541 gttcttcctt ttaactcgta tactggaggt attttggcca taatccatca aactcctttg
    12601 cctgatatta actatttaca ggcaaaaatc attgacctta ttaattatac ggctttctga
    12661 attaaaaaca aattttttat agattggaca cttgatatat aatacgcaat taaatcctta
    12721 aagatttcaa ataacgaaat acatcaaaaa aacctaaaaa aacaaaaaaa attaacattt
    12781 ttctgttttt ttgtatggac acatgccata taaaacaacg ataaggtaat tctatcagct
    12841 cttcttttta tgataagagg atattacagg tcttttaatc tgaagtcttt aagacaggac
    12901 catactcttc ttcaagcttc ttagccgcag cagagccaag acttccccca gctttctctt
    12961 ttgcggcttt aagcatttca ccgggattac gggccttatc caacagttca tccagatcga
    13021 ttatctcttt atcatagagt tcccataaat ggtcatccag aagctgcata ccaagtttct
    13081 tgccgatctg gatgctcgat tcgatacgat agactttatt ttcacgaatc aggtttgaaa
    13141 tagccggcgt aacaatcata aactcatacg ccgccacccg cccgcggccg gtttttttcg
    13201 gacaaagcgt ctggcttagt acggcaatta aattggtact aagctgcacg cgaatctgct
    13261 cctgctggct gacaggaaac gcgtcgataa tacggttaat cgttccttga cagccagtcg
    13321 tgtgcagagt tccgaacacc aagtgtccgg tttcggctgc cgtaatggcc gcttccatcg
    13381 tttccaagtc ccgaagttcg cccaccagaa tcacatcggg gtcttgacgc agagaacggc
    13441 ggagggcctc cgcaaatgtt gtaacatcca gacctacttc acgctgattg ataatggatt
    13501 tcttgtgctg gtggtaatat tcgatgggtt cttcgacagt gatgatatgc ctgtccagtg
    13561 tttcgttaat ataattaatc atcgccgcca gagtggtcgt tttaccgctg cccgtcgggc
    13621 cggtaaccaa aaacaaaccg cgcggacgcc ggcacaaagc cgcgcaaata tgcggcagac
    13681 cgatttgttc cagactcagc agctgcgacg gaatcagacg cagcaccatt gcaatattgc
    13741 cgcgctgctt gaacactgct gttcggaaac gtcctgcatc tccaaaggca aatccatagt
    13801 cggtacttcc cacttcctgc aattcctgct ggttgcgttc cggcgtgatg cttttcatca
    13861 gcgcaaccgt atcctccggc tccagcactt tggtatccaa agaccgcaga cgtcccgaaa
    13921 tacgaaggac cggaggacgc cccaccgtaa tatggacatc cgaaccgccc attttaatgc
    13981 acgcttccag caatcggtca atattcaaac ttgccatatc aaaacacctg tcctaaaata
    14041 caaaaaatga ttcctataca tcgacaacca gttcttctgc ctgtgctacg cgcgatactt
    14101 catccggcgt tgtaatgcct ttgaagacct tgattttgcc gtcttccagc agcgaacgca
    14161 ttccggaagc caaagcggct ttccgcaatt cactggcggg cgcacgcgaa aaagccagtt
    14221 cccgaagctg gttgttcatc tcgagcattt caaaaatagc gatacggccc ttgtaacccg
    14281 ttccctgaca tcgggcacag ccggcacctt tataaatcgg atgcttatga atatcctccg
    14341 gagtaatccg cagcagccgc aggtaatgcg ggtcgggggt ggtatctacg accttgcatt
    14401 ctttgcagat gacacggaca agacgctgag ccataatcgc ctgaatggaa ctggccacaa
    14461 ggaagggctt gacccccata tcaatcaatc gcgtgatggc gctgggggca tcgttggtgt
    14521 gcagggtact gaagaccaag tgaccggtca gagcggcctg aatcgcaata tccgctacca
    14581 cgccgtctcg aatttcacca atcagaatga tattgggggc ctgacgcagc atagaccgca
    14641 gaatggcctc aaatgtcaat ccgatttccg ttttcacctg acattgatta atgccggaaa
    14701 tattgtattc gacggggtct tctgctgtaa taatcttctt atccggcctg tttaattcct
    14761 gaagtgctga atacagggtt gttgttttac cggaacccgt cggacctgta acaagaaaga
    14821 ttccattggg ccgccgaatg atgcggataa accgctcgta gtcatccggc tcaaaaccga
    14881 ttgcctgaat gccgatattg acggattcgg gccgcaaaat acgcagaacc gtactctcgc
    14941 cgtgataacc cggcagacag gatacacgga agtcgatatc ctgtccatca accgtccgct
    15001 taatacggcc gtcctgcggc agtcgccgtt cgccgatatc cattcccgac ataattttga
    15061 tgcgggccag cagcggcgcc tgcatgtttt tgggaatatt gtcccgttcc acgcacacac
    15121 cgtctatccg gtatcggatt cgcacccggt cagccatggg ctcgatatga atatcgcttg
    15181 cccgcatccg aacggcttca tcaataatca ggtttgccag acgaacgatc ggcccatcgt
    15241 ccatttcgtc ttcggccgcc tgtgcccggc gaacctcggc ttctatcgta tgccccatag
    15301 ccgccagttc cgcggctgtc tggtcaatgc tgctgcggta atcatctttg gcaggttcat
    15361 ccgcttctga tttattgaga aaattttcaa tcttggtggg gcttgccaag cagcagcgga
    15421 tttccatatt cagccgaaaa cgcagcaaat ccaatgtatc caaatccatc ggatctttga
    15481 tgacaacttt taactccccg ttattgactt cgatagggat gattttatga cgcttgatga
    15541 tatcttcggg aactaattta gcaacatttg gcgggatttg gacattatcg agatttacaa
    15601 attcataacc gaattgttta gccagacact cgtaaatctg gtcttcggtg gccagtttca
    15661 tctccagcag cacttccccc agccgtttat tggtttgctt gcctttttta attgcggcga
    15721 ttagattctc tttgctgaca taccctttct tatagagaat ttcgcctaag tgtcttcttt
    15781 ctttcgacat tggatctcac ttattactgg acaaaaaaca gtaaagcaca gaaatgtatt
    15841 gtaccataaa aaaaacggct cgtcaaacct gcaatcgcag ccgaacaaga tttaatgctg
    15901 tcatcgccgc ccgctgccga atagactgcc ggtctgcatc tccaaaccgg catctttgca
    15961 catcacaaat gccgtccgtc cataccccta taaacaccag ccccaccggc ttttcttcgc
    16021 tgccgccgtc cggacccgca ataccggtaa tagaaacagc tatatcagca ccggatctct
    16081 ttgccgcccc ctctgccatt gccgacgcaa cctgccggct tacggcgcca aaatcccgaa
    16141 tgaattgttc gggaactccc aattggcgca cttttgcttc attgctgtaa gttacccagc
    16201 cggccagcat ataggcactg gagccgggta tatcggtcag cattttcgat aacaatcctc
    16261 cggtacagga ttcagctgcg gctatcgttt tgtgcttctc tcggagcagc tgccccacag
    16321 ccgcaggcaa actcgagtca tcttctccaa atacgatatt cccgagcaat gccttcaatc
    16381 gctgcttatc atcctgaacc attttcattg cctgcatttt actgtcggca gatgcaataa
    16441 tgtgaagtac gacctctccg gccccgcagg tacaattgat caggggattt cgcctgcggt
    16501 tcattaaatc gcccaatcgc tgtgcaatat cggattcacc agcaccaaaa caatgcagct
    16561 tgccggccgc tgtaaacgca ccttgtgccg ccgtatcgat ttggtctttg acctgctcat
    16621 tgaacatgcg cttcatctcc tccggcactc ccggcatggc ggcaaaaaaa acaccgttgc
    16681 gcaaacccca gaacccgcaa gccgtacccg ctggattatc aagacgccgg cagccggcag
    16741 ggatataggc ctgcgactga tttcgagcag gcatagtccg gcctcgcttc ttgaaaaaat
    16801 ctgcaatttt gtccagccac tccggctgaa atacaagatc ggtcccaagc caagaagccg
    16861 ccgcctgccg tgtcaaatca tcatccgtcg gccccaagcc gccggttatc agaaggattt
    16921 ctgcctcccg tgatgcctca tcgaaggcat gaataatccg gctgatttca tcgggaaccg
    16981 tccagactcc ggctgttgga agacccatcg ccagaagttc ccccgccagc cacgaggcat
    17041 tggtatccgt tgtatgaccg ttgagcagct cattgccgat gctgataatg ccgactttct
    17101 tcatacttta attaaaacaa agcagcggct gaatgtccag ccccaaacct gtctgccgcc
    17161 ggcaggatgg tctgccaagg attccgtttt cctgataaga taaaaaaagc ccggctgata
    17221 ctgatgccgg gcttagaatc tgttttgagc agaaccgagt tatactgtct gctggacttg
    17281 gacctgctga tatttctgat aagaacgata gacctcccgg aaatatccgc ctttgcggct
    17341 cttggctccc atcaagacag ctccggcaac attggcattt atatccttca gttcccgcag
    17401 gatccgcagc gctgccccgc gacgggtaac agcagcattc agcaccacca atgccgcgtc
    17461 ggcctgtgcc gccagcgtct tggcatcgct gaccagcatc gcaggtccat caatgatgat
    17521 ataatcaaac tgggatctgc agtaatccag cagcgttttc atccgctcac tggtgaacag
    17581 ttcagccgga ttggccggca gcggcccgct gtcaatcagg tacaacccgg tgatgccgct
    17641 gtcccgaata atgcgatcct cggaacattg tcccatcagg taattgctca ggccgaaatc
    17701 cgaaaatccc gatgaaccat tggcctgtga gcgggggaat aaccgcgctg tggacggccg
    17761 gcggaaattg gcatccagca gcagaacccg gcggccttcc atcagcaggg tgctggccag
    17821 attgacggca acggttgttt ttccgtctcc ggcctgtccg ctggtcacgc acaatacctt
    17881 gtgcattgct ccacccggcc ccaacagacg caaattggtg cgcagctgcc gatagccctc
    17941 gctcatcatc gaataagggg cttgacgaac cacatgatac aaatcaaccc cttctgcctc
    18001 atcatcatcg ctgacatgcc aaatcatccc catcagcggc actctcagat gccgcaccac
    18061 atcgctgggc gtgcggacca aatcattagc cagctcaatc aggaaagcca gtcctaaacc
    18121 agccagcagg cccagaataa agccgcccgg gaagaacagc tcccagcgcg ggaaactttt
    18181 ttcccgggga tctgttgcgc cgtaggggga cgacagcttg ctcagacgcg gattgtcatg
    18241 ctgggcattt ttcttctcaa tcagggtgtt catatcctcc agaagcttct gattttcttc
    18301 ccgctgctgc tcataccgcg catattcagc acgaattcgg tcgatttctt tgtattcggc
    18361 acgggccgca ttgagctgct gggttgcagt ctccaactgc gaagtcagcg ctgtcattgt
    18421 atcccgtgca ttgatcagat tggactggcg aatcaattca ccaatcatct tctgacgggc
    18481 atcatactca tcgtacatct ttttaagagc cgcctgcgtc tgtttgacaa gacgatgctc
    18541 atcgccgaaa cgggcccgct gctgatccag cagcggttcc attgccgcta tattgcttct
    18601 catctggcgg gctatcggat cattttcaac ttcctgcctg accacttcat caaaatcata
    18661 tgccttggcc cgcacatcca aggttgcaat gatactttcc agccgcgatt tttcgctgtc
    18721 atattcacta tatcggtttt ccaaatcagc cagcttctca tccatataat cccggaaaga
    18781 ctgattttcc ccaagattca ggcgggcaaa ccgcgtgccg gaacggatgg attccagact
    18841 ggcttcaatc tgattcagtt tggtctgtat ctggtcacgc tggtttttaa gctgagccag
    18901 ttcctgcttg agcgtgcttt gagccagttc ctgctgctgc cgcataaaaa ttcggaccat
    18961 ttcatcgaca attgttttag catcccgggc attgcggcac ttcatggaaa ccagcagata
    19021 gttgttgtcg cggggggcgg ttgcactgag attatcctgc aaatccttca aggcctcgcg
    19081 gatagctttt tcaacatcgc cgacaatctg gccctgactg tcgatcttgg caaaactctt
    19141 gaaccagtct gtttcccgga ctctcggctg ctccagcaag gtcagcagca tattctgctg
    19201 ctgaatcatc gatgccttgg tgaagcgaaa tgcatagtag agatccttct gcacctgaat
    19261 gctggctatc tgcatcggat ccatatccac cggcgggtcc acatcgattg cacgaacaga
    19321 cgtgtagcgc ggataataac ggtcgcacaa ataccacagt ccgccgccga taaccgtgcc
    19381 cagaatcgtc atcagtataa tcatccacag atgccgccgc agaatgccca aaatctcccg
    19441 cggggtcaat tccataccgc cgccggcccc gccggcaggt ctggccggcc cctgcggggg
    19501 ggtcatcggg ggacgggacg atgccggccc ggtcatcata ttgattgtcc gctggattgc
    19561 attatccata tcaagtctcc cactacaaaa ccgttttatt attgctgacg cagccgctca
    19621 atcgccttct gaagctgctg tttttcctta tccggcattt cctgtgcaga atcaagggct
    19681 ttttgatagg tctccatcgc ctgctgcggc ttattgagcc cttcctgtgc cattgccaga
    19741 tgcttataaa catcccacgg aaccggctgg ccggaaacat cataaatctg aatcgcccga
    19801 agcagattct gttctgccgt gtcatactga ccaactttgc actgaacaaa tgcataggta
    19861 tccagaaaaa ccggatttcc cggatcctgc tgatgagcgc tgcgtgcata attcagcgct
    19921 ttttcaagct gctggtcgtt gtcagccagc aggtaggcca tgttgttcag cagcgacgaa
    19981 ttgttcggct gaatctgaag cattttctcc agcatgtccg ccgcgcgttc aaaatagctg
    20041 ctgtctgccg ttttcgcata ggccatgatc agcagattga ccttcttgag gttatacagg
    20101 aacagctgcg gattgtcggg cgcaatcaaa ccgatgcact ggtctgcata ctcaatcgcc
    20161 ttattgtaag cactcttttc ctgcgccaac cggcaggcca acagcagggc cggcagcgat
    20221 ttggcatcag ccgccagcgt tttcgttacc cagccgtcca gcacttcttc tccggccgcc
    20281 ggccgaactt tctgcagaat cagctcctgc atagtctcgc tggtctgggc cttgtccagc
    20341 gctttataaa agctgtcaat cgccttgtcc ttctgcccct gctggctctg aaccagtccc
    20401 agatacatat acgccaccgg tgcccccggc gtatcaatca aaccgccggc aaaagtgata
    20461 agtttatcat attgcttggc tgcatacaag acttctaaat atccctgtac caactcgaga
    20521 ttcggctggt tttgggcact ggccagcttc caagccgaca gcagcaattc ctcagcgcgg
    20581 gtgtaatttt tctggctgag gtagtacatc cccgcccggt aatgccaaaa cgcactgttg
    20641 ggatattttt ccaaagtccc ctgataaaac ttgtccaaat ccgcagcacg gttattcttc
    20701 tgatacagcg cttccagcat cagccgcacc tgaatcgggg ctttcgggtc atccagtgcg
    20761 gaggccagtt ctccaatggc agcggttatc tgccgggtct cgttatagac attggccagc
    20821 tccatccgaa ccatcggatt atcctgaagg cctttgctcc gctgcaggtc ctcaatggct
    20881 ttctgctgct gattcatcag ccgatacagc cggccgcgca gccgccatgc tcccgcattg
    20941 gcggtatcct tttccagata cagatttgtc agcgaaagcg cctcatccag ccggccatgc
    21001 cccatcgctg tccatgcttt tatcagaaga atggtaatgt catccggata cctctcctgg
    21061 aaactgggga ttctgctttc cgcctcggca gtaaaagcat tgtccagata tttctgcata
    21121 atccacaatt cgccgtcttt ggtttgctcc agcttgacca gcgcatcaag atactctttg
    21181 acctgctgac gcttgccggc gccctcggag gcgatcacaa gtccctgata cagcagtaca
    21241 tcatcgggat gtttcgccag aaggtctttc aagatcgcct cggcctgttc aaactgcccg
    21301 ccgcgaaggt aatacttcca cagcagattc tggtcgcctt tgagcaaatc cagcgcttta
    21361 tcgctctgct gggtcagccg gaggtagtcg gcatacgcct gccgaatgga atcattgtcc
    21421 ggctcaaggt ctatcgcctg ctgataggcc ttgcctgcca gctcaaccaa tccggtccgc
    21481 ttggccgtat cccattccga ctgcgccatg cgaagggcca tattgcccag catcagggca
    21541 ttggaggccg tcggaacagt ctgcagcagc cgctgacgga tctgcaaagc ccgatccggc
    21601 gcctgctgct ggatgctttc ggcatagagg ccctgcagct gccagtcggc cggattaagc
    21661 agcatcgcca gcgtcagcgc ctgttcggct tcgctgcgct gttccggcgt cacttttgtc
    21721 cccagttcat tgttccgcgc aaacagcagc atcgccagat tccgggcata cgcagcatgt
    21781 tggggattca tctgaaccgc ctgccgggcg ttctgaatag ccgctgtgct gtcctgcatc
    21841 tggtgctgaa cctgactttt cagataatac aaataactgg acagcggacg cagggtcagg
    21901 cactcatcca gacggcgcag cgcttgggga taattttcct tgagcatctc cacccgtgca
    21961 gccgccagcc cgccttcgca tccgtcggca tttttcgaac gaatcatctg gagcagacct
    22021 tctgccttgg cactatcctt ctggtcaacg gcgatatcaa attgagcatt cagaatctcc
    22081 ggatcattgg ccgcatccgg cccggcatcc tgcatcagag ccgccatcgc cttttcatag
    22141 tctccctgtg cccgatagag ggctgaccgc agcagggctt tctgccgggg attctgaatc
    22201 cggtccgcgg cctgctcctg cagctgcgct gtcggctgcg ctgccggatc gtcctgagac
    22261 gcccgcaggc gaagcagctg caaaccgtaa gcatccggat tggctgccag ataaatatcc
    22321 agaaaggcag ccgcctgcgg ggcctgcttg ttttgaatca tatccataca aataacattc
    22381 aacagctggg gatccatctg agccggctcc agctgaagcg cctgctgcaa aaccgtattc
    22441 cgctcagccc gcagagtatc gagcttcttg agctgttcgg ctgtgggctc cttgggcggc
    22501 tgtgcctgcc ggccttcggc aatagaaccg gcaatctggg cgatttgggc tgtcagcact
    22561 tccagccgca gctgaagcac tggggcagac gtcccatcca gctgggccag cagacggcgg
    22621 gcctgatcaa atttgcccag tgccgccgcc gcacgaatcg ccagcatttg gctctgtcca
    22681 ttggctccat agcggttttg atactcctcc accatccgtt ctgtattgtt ccacgcacgc
    22741 agctggccca gaacctcggc ataatccaga atcagcttgg gcttctggag cacatagcgg
    22801 ctgttgttgt agattgccca ctgcagaaat tcgcggcgca tacccggttc gccgctgtct
    22861 ttcatcaaat cggcaagcac cgcacagacc agcggatcgg cattggaaaa ttcctgcggc
    22921 ctgtccagcg cccgccactg ctcataagcc ttatgcagaa gacgcagacc cttgtctttc
    22981 tggtccttgg ccagagccag cagaccctga tacttctgcg cggtgggact atccgatgtc
    23041 cccagaatcc cgctgataac agcaacgcac gattcggctt tggctgtcag ctcatcggct
    23101 tcagctgttc tgccttcccg ccgggctgtc agcgcatggt caagatacag atccgccaaa
    23161 aacgcattca gcgtaaaccg gtaattgaga ttgcgtccct gcagcggtcc cgtcacgtcc
    23221 tgcacggcat ccatcgacag cgcatcctcg gcaatctgcc gggcatctgc caatgccgcc
    23281 gcatccgcga aggcattggc cttgcggtac ataagccgtg ccagacggag gacatactca
    23341 aaattatcgg gcttcatctc gtgtgccctg cgaaccgctt caatggcctg attgatttcc
    23401 gcctgcgccg actgcctgcc cgggaattca tacgcgatcg agacaaccag ccagaacaca
    23461 tcgttgggct gaatctgcgt ccggtatgtt tcaatttcag cccggaatgc ttccagccca
    23521 ttcggatcgc cgacggttga ctgcagccga tagcgaagca gattggccac cgccgccgat
    23581 ttgtcatcgg ctcgctcgac gcctgcctgc agatgctcca aggccttctg ccgtgcattt
    23641 tcggcagccc gaatcacacc ggattgagta tccagttcgc cctgaaggcc ggccgcttca
    23701 gccagtagca gatacacctc cgcattgccg ggctcttttt ccttcagctg ctcgaggatg
    23761 gtcatgcagt cactcagatg ctggcggcga tcggtggttt cgccccgctg ggcaatgtac
    23821 agcagcgatt tggcgtgggc tgtcaaaagg gtcgtgtccg gctcggcttg gccggctttg
    23881 agcgtatcca gcagcttggt ggtgttttcc agaacattct gccacgcccg tgcgtcgccg
    23941 gcttcggcgg cctggtagta aaagtccagc agcttgcggc gggcttggac attgtttgta
    24001 tcaatcgata caatcttgct ccagcagccc atcgccttat tccattccgc ctgatgctgg
    24061 gcattgtgaa tcaaatggaa atccgccagt tcaaacagcc gttcaatctt ataggcatca
    24121 tctttgccgt atgcaaaagc ccggcccagc tgcgactccg cttttttata atcccccgcc
    24181 tccagtgcct gacgcgcttg ggccagtgcc cggtccggat cgcggtggac tttcaggtac
    24241 actacgcccc ctgccagaac acacacaatt ccgaccagca gaatgccgac gacaagtgct
    24301 acttttttat tcagtttttt tgccatacag tcctctattc gaacgaccca tcgcggttac
    24361 tgtctttgcc gctgctgcgg ctttgcattg gcggcttccc agtccggcca atgattggct
    24421 tccagctccg gcagcagccg ggccagcagt ctgcggctgg cttccagcgt ctgctcctga
    24481 ctgggataaa cgaccccgaa ggcatcggcg ccgtaaaatt tccattccac cttgcagaaa
    24541 tacgaatact tgctgaacca gttgctgccc agaaccagcc gtgtctgcgt ccggtcgccg
    24601 cagtaccggc cgttggcgtg aaagaaatac tgcacggaaa aattgtcttc tttggctgcc
    24661 atggtgccgg cccggccgaa aatgacatac tgaaatgcaa gttttcgctc cccctgcggc
    24721 agcggaatat cgagagtcag cgtctggccg tctttgcgct cattgccgcc gccgacatag
    24781 cactcatccg gcacatgcgg caccatatcc ggattgccgg tataatacgt aataaacagc
    24841 gagcagaaac gcagcggact gcccgccggc gcttcgggat cttccaaaat ccactgcaga
    24901 tactcttccg tgccgagact ttccagcaca tcccggttga gaatctgctc cttctgcttg
    24961 accacaaacg gccgcaacgc ctgttcgttc atctctgcca gcggatgccg cagttcgatg
    25021 cgctgcttgg tcatctgcac gcccagcacg cggataatgg tttccttggc ggctgcagcc
    25081 gtcagcagaa gagccgtaca gactacaaac gacggcttca aataagattg taaagcagat
    25141 gaggccactt aggtttcctg tgattgccgg ggtacggact tgcgaacaat aatatcttcc
    25201 tgcgtctgtt cttcctcaac aaacaggttg ctcatcagcc aagccaatcc cccgtagagc
    25261 gcaaatgcca gcggcagcat cagcattccg agcatatcat ggtaaatgcc ctgcgcatac
    25321 ttgggattgc ccagcacata aataaatcct gtaacagtaa cgcgcacaat attgcagaga
    25381 atggctatcg gcacggtgct ggccagcagc accagccgct gccagatcgg acgccaatgc
    25441 aggtatgcca tcgccactcc cagcgccaca aacgccatca gcagccgcat cccgctgcac
    25501 gcatccgcca catccagcgc cggctcaagg gctttgccct tgtacagcac atcaatcacc
    25561 gagccgtgca cttcggcctc cagctgcggt acaaggttca ggataaccgt ggccgcctgc
    25621 gctgccagct gacgcatcgg attggtcagc tgaaaataca gacgctgcgg cagcggcacc
    25681 gcaaagaaca aaaatgccac cggaagccat gtataacgca gcagcttcca tcctcccaaa
    25741 aacagcacca ccgctccgat gactgcaatc atcgtcagcg gcttgccgta cataaacttc
    25801 agcatcacaa ggttgatcgg atgcagcgcc agcagaaaca gcagtgctcc cagccctgca
    25861 aaccagctgg gacggacctg ttgaatcgcc agaatctctt ctttgttttg atggagaaaa
    25921 tacagactga acagcggaat caaaaatcca tgcgaccagc tcggattgga aatccactgc
    25981 agcaccagac cgttaatctc ttcgcggaaa atccaccaaa tcagcagggc tataattgct
    26041 gcgcggatat acagatggat acccaaatcc agccagctgg gctgtttttc ggtcctgtct
    26101 gcagccacca atatgtcttg tttctgattc attctgcgcc gtccgccaag cttagaacgg
    26161 aagcggatct cccaaatagt cgcggtctgc aaagttgcgg tcataaataa atccgaagcc
    26221 gtacgtggcg cggaatgcgt ttcgcagaac agccagccag cggctggtcc catgcgtgcc
    26281 gacattgatg tagtcatacg gtttgatgaa aaagtccggc tgcagccctt gagcaatctt
    26341 ctgcagatcc accagaacaa tttcttcctg catcaggccg gccttgtttt tgccgatgcg
    26401 ccgaacaact tcaaccttct ggggccatgc aatcgcattc aggccgccgg ccgtggcaat
    26461 cgcctgcttg agcgtgatag gacggccggt aatcgagtag gcattcgggg cattgacatt
    26521 gccggccacc cagaactctc ccaccaaatc ccgcggcacg gtaatccggt ctcccggacg
    26581 aataacaata ttataccgcg gatctccgga ttccagacga tcccgcggaa tctgaataac
    26641 acgggttacg gtgctgcttc ggccgacctc cgaaaggccg aaatccgacg gtatctgccg
    26701 ctggggtttc gtctgggcgt actgctccgg ccggagcagc tccggctgct gcggcgattg
    26761 ctccgaaggc ccttccactt ctatcggctt ccacttgccg tcttcaaaaa cccattcgat
    26821 ccgtttggat gtctgctgcg gcagcagagc ttcagggctt ttcacatcgg ttactccctg
    26881 caccggttca atgccttccg gtgctgccaa tgcctccaat tcgccttgcg ttgccatctc
    26941 ggccgacgtc acaaacaagc cgccgggccg gcgggtctcg ctgggcgtaa tcagctccag
    27001 catctcatcc tctttgccag gagccggctg ctggggcttg gaatccgtgc ctgcggcgcc
    27061 gggctcaaca tcctgctgcc tgacatcgcg cgacacataa atatagctga cattgaagtc
    27121 ccccattccg cctgccagcg ctattgcgtc cgtcaggcga atcggatacc gcggcagcgg
    27181 ataccgggcc gattgagcaa ctccctgccc gataatcgaa aaataccgct gctgggaggc
    27241 catcagggtg acggttacag acggattttt aagaatactg ggcgacagaa tactggcaat
    27301 ttcccgttcc agctgcgctt cggtcagacc agccgcc
//
LOCUS       DAOTBO010000027        26371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1516_length_26371_cov_5.522762, whole genome shotgun sequence.
ACCESSION   DAOTBO010000027 DAOTBO010000000
VERSION     DAOTBO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 26371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26371
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_26371_cov_5.522762"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..215)
                     /locus_tag="PLE88_05045"
     tRNA            complement(145..215)
                     /locus_tag="PLE88_05045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Gly,seq:ccc)
     gene            complement(509..1666)
                     /locus_tag="PLE88_05050"
     CDS             complement(509..1666)
                     /locus_tag="PLE88_05050"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOM75869.1"
                     /translation="MRTILKGAAVLLVVSVFLTAGCAGPSAKTAAAKLDITKADFGTT
                     SDGKKVDIYTLTNTNGMEVKITNYGGTVVSLRVPDRSGKLDDIVLGFDNIKDYEQKSP
                     YFGCIIGRYGNRIGKGQFTLDGVKYQLATNDGENHLHGGLKGFDKVVWEAQPIKNAKS
                     VGLKLHYLSKDMEEGYPGNLDVTVTYTLTNKNELKIDYVATTDKATVCNLTNHSYFNL
                     AGQGKGDILGHELMLNADNYTPVDKGLITTGQIVSVKGTPMDFTKPTRIGAGINADDQ
                     QIKYGGGYDHNWVLNKKGSKMTLAARVYEPSTGRLMEIYTNEPGIQFYSGNFLDGTLT
                     GKEGRVYKHRYAFCLETQHYPDSPNKAQFPSVVLRPGEVYKTSTVHKFSTK"
     gene            complement(1851..2624)
                     /gene="nuoB"
                     /locus_tag="PLE88_05055"
     CDS             complement(1851..2624)
                     /gene="nuoB"
                     /locus_tag="PLE88_05055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOM75870.1"
                     /translation="MIHSLIARIKQGYRTYPFPDKPPVLPERFCGRPRLHPNLCNQCG
                     ACQKTCPVNAIDCVNDRIRIDLGKCLFCGDCAKACTRSAIVFTGDYRLAVNRREDLLV
                     SDIEPKLAEAMNQQCRRIFGRSLKLRQVSAGGCNACEADTNVLTTLVFDLGRFGIQFV
                     ASPRHADGILVTGPVTQNMRYALEETYAAVPSPKFVIAVGSCAIAGGPYSGHSEICGG
                     VDALIPVDLYIPGCPPHPYTILDGLITFLGRRQPQNGKD"
     gene            complement(2632..>3960)
                     /locus_tag="PLE88_05060"
     CDS             complement(2632..>3960)
                     /locus_tag="PLE88_05060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOM75871.1"
                     /translation="RRGLLHLASALVKDSYPALTPDCPQAHWFEREIAEQFGVRPQGH
                     PWLKPIRYHKSYTGRDAWARPADAPILPCVTDFFRVEGSEIHEVAVGPVHAGIIEPGH
                     FRFQCNGENVLHLEIALGYQHRGIESRLIGAPDKRSIHYMQTAAGDTTVGHTLTYCHV
                     IEALSCCSVPIRAHVLRGIALELERLANHTGDLGALAGDVGYLPTASFCGRIRGDFLN
                     LTALLCGNRFGRNFVCPGGVGFDLEQRHIDDLRSRLTATVKDTACAVDCLWSTPSVLS
                     RFDGTGTLPRQTAVELGLVGPAARACGLDRDCRYNFASGIYQFHQMPVAAWNTGDVFA
                     RAYVRWLEIQHSAAFILNQLDTMPAGSVHQSLADRMPNKIAVALTEGWRGEICHAAVT
                     DSTGKFVRYKIADPSFHNWMGLAVAMRGQQISDFPLCNKSFNLSYCGHDL"
     assembly_gap    3961..3970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4138..5607)
                     /locus_tag="PLE88_05065"
     CDS             complement(4138..5607)
                     /locus_tag="PLE88_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOM75872.1"
                     /translation="MLWMLIFVPLAAAVASFLIRSDALRRMLLIAAALCHTAAAAACW
                     VYPTGPILGGWFDIDPIAQYFLTLTSVLFLAGAFYAVGYLKAEQTGRKKDFMEDAFFI
                     NAPQAVFIGCLLLFLTTMTMVLASQHFGLLWVAMEATTLVSAPLIYFHRHHRSLEATW
                     KYLLICSVGIALALLGNFFLAIAASGTQTESISLLRNALIANGSKLSTPWIQAAFIFF
                     LVGYGTKMGLAPLHTWLPDAHSEAPSVVSSLLSGALLNCAFVGILRAFEVACAAGQTA
                     FAQDLLVLFGLVSIAFAAVFIIGQADYKRLLAYSSVEHMGILALGTGLGQAAVSGAML
                     HAVNHSLTKAVLFMTAGNILAVYKTKTASRVRGLFSVMPVSGLLWVAGLLAITGFPPF
                     GTFLSEFTILQSAVEQGYYGTAAAFLILLGFIFIGFSAIALRMTQGQKPESVQPACER
                     PPAVVPPLVLITAVLLLGVYVPEPVNDMLHRAAVLLGGR"
     gene            complement(5607..6242)
                     /locus_tag="PLE88_05070"
     CDS             complement(5607..6242)
                     /locus_tag="PLE88_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75873.1"
                     /translation="MWTDISMVILILTNMAVLGTSRLRACIRIVAVQGVALGILPLLI
                     HRFEPGLLVFAFLSIILKGIIFPMMLGRTLRQLNVWREDAPYVGYGLSLLVGPLLLAF
                     SSWISPRLPLPYSVHSPMLIPVALFTLMNGLYVIVSRKNALTQVLGYLVLENGIYAFG
                     MAVAYHQPLLIELGILLDVFVGVFVMGIAIFHISRQFDHIDTDQLTMLRDH"
     gene            complement(6246..7163)
                     /locus_tag="PLE88_05075"
     CDS             complement(6246..7163)
                     /locus_tag="PLE88_05075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOM75874.1"
                     /translation="MMQTAMIIQPLLAITLAPLLLGVINRVKAFFAGRRGQPLFQLYY
                     DLWRLLHKGAVYSTAASWIFRAAPVVSLAAFLTAACIIPLGGVRSLFSFSGDLILAAY
                     LFGLARFFVVLGALDTGSSFEGMGASREVHFSALAEPALFIALMALARQTSSLSLSGI
                     FSSITPDFWTAHSATLLLVSAALAIVLLAENARIPVDDPNTHLELTMIHEVMVLDYSG
                     PDFAFILYGSALKLWLYAALLTGAILPLGTLPPLAAAAVSAAAIFLTAVFIGIVESSM
                     ARLRLLRVPHLLFTAAALAILALVFALRS"
     gene            complement(7160..9124)
                     /locus_tag="PLE88_05080"
     CDS             complement(7160..9124)
                     /locus_tag="PLE88_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOM75875.1"
                     /translation="MAFILYGIGLIAASGFLCLFIRNGRICTRIGAAGVLAGSLLGLY
                     GAGYVLLSGTILSLDLPWQVPYGAFHIELDRLSSLFLMPLFVVCSVAAVYGCGYMAAY
                     KDRRLGVPAFFFNLLTAAMAMVVLARNSILFLVSWEVMTLASYFLVTFEDEHRSVRQA
                     GWVYLIAAHIGTAFLLAFFVLLGKYNNNWDFAGVDVGDRQAAILFLLALVGFGTKAGL
                     MPLHVWLPEAHPAAPSPVSAVMSGVMLKMGIYGIVRTISVLPDVQIEWGWLLTGLGIL
                     SGVLGILFAAAQHDIKRMLAYSSIENIGIILIGLGLGIVGMAGQSPALTVLGFSAALL
                     HTVNHALFKSLLFMGAGSVVHATGTRLIDVLGGLIKKMPFTATGFALGAAAICALPPL
                     NGFISEFLLYYGSFKAVLSERIEIVVPAAAAIAALAAIGGFALAAFAKVFGIVFLGLP
                     RSQRAQVAHESPLSMTAPMGVLALLCVMASAAAPWLFSSIACVVSSAGFSKAADDPIP
                     AARPMLYSIAAASILLLMLAAGAALLRWKILRGRTTAEEQTWGCGFAAPTSRMQYTGS
                     SFAQPIVDMFRAVLGSRRKVVRITEFFPRQAEFETHTPDVSSEYLYRPIFKWVDWFIG
                     KMRWVQYGIVQVYVLYIALTLMILLIWKLR"
     gene            complement(9125..9841)
                     /locus_tag="PLE88_05085"
     CDS             complement(9125..9841)
                     /locus_tag="PLE88_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM75876.1"
                     /translation="MQLTVRDVARLFAVSEKTVYRWLDQKTLPVYRVNNMYRFNRAEL
                     LEWAMANRINVSAELFSEPESADSPLQGLGQALRAGGIYYRVGGTDKASALRSVVELI
                     HLPPEVDRQFLLNVLLAREEMASTGIGDGIAIPHVRNPIVLHVPQPTVSLCFLEKPID
                     FKAIDGRPVYCLFTIVSPTVRAHLHLLSRLSYALRDAALKHAIEIQASRDEIFSHIGR
                     IEADLDAPQSPLPDQQKEKK"
     gene            complement(10003..11325)
                     /locus_tag="PLE88_05090"
     CDS             complement(10003..11325)
                     /locus_tag="PLE88_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOM75877.1"
                     /translation="MQAAPQRILSLFDAVCIAAGIIIGAGIYQITPAVARGAGTVAQL
                     MSLWIIGGLLSLCGALSYAELAAAYPNSTGGDFVYLTKAYGRPAGFLFGWSQLVIVRP
                     GDIAVMAFAFATYGRQIWEPAAVDPQISQRLYAAGAVVVLTLMNMLGVRQGKWTQNLL
                     TAVKAVGLIGIAAAGLAVNRPVEPARPLEPIPAGLALIFVLFTYGGWNEVAYVAAEVK
                     NPGKNIVRALAVGMAAVMILYLLVNGAFVYGLTFGGLAASEAPASDMLACLLPSGGRQ
                     LISALICVSALGAVNGLIFTGARISYAMGSEYRLFRLLGNWNQHTGGPVWALAVQGSI
                     AVCLIVVFGGYMDAILYTAAAVYAFYMATAFSVIVLRYRQPQVPRPYQVSFYPLPPLI
                     FCIVCAFLIYNSVSYAWAFRPASLLVLATAAGLGLAVYIAVERYNYNC"
     gene            complement(11373..12236)
                     /locus_tag="PLE88_05095"
     CDS             complement(11373..12236)
                     /locus_tag="PLE88_05095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM75878.1"
                     /translation="MRKIVIAAGIAAGMVCFMLAAGPEVQPVRPGGEGAGADLERWLN
                     DLEQARRDNNLEKIDQLISLARQNRQIFRPAIQATGPDRQPGAGRRISQNQQDIQFQK
                     DPLAKNENEKTILAVLEDILQNQRAMNVPQSDGRLLRLLAEFANAKNIVEIGTSTGYS
                     AVWFGMALQKTGGKLTTFEIDQRRAAAARANFKRAGLDDCITVIEGDAHKTVQMLKEP
                     IDIIFLDADKDGYIDYLNQLLPLVRPGGLVIAHNITPGMADMRFIDAVTGNPELETIV
                     RSGVSLTLKKR"
     assembly_gap    12373..12382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12660..14099
                     /gene="purF"
                     /locus_tag="PLE88_05100"
     CDS             12660..14099
                     /gene="purF"
                     /locus_tag="PLE88_05100"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOM75879.1"
                     /translation="MADKKEACGIFGIFGDPDAVHKTYFALHSLQHRGQESAGIASSD
                     GELIHCYTGMGQVSRVFREGRGILERLDNPIAIGHVRYSTSGSANIANAQPFLSEYSR
                     GQVAVAHNGNLINASLLRDEYEAYGHIFKSSNDTEIIVHLMAKPTHVTKPDPLGHVLN
                     HLQGSYSLVFLFPDRIEAARDPYGIRPLCLGRTADGFYCVASETCALDAIGADYVRDV
                     EPGEIVTLDKHGLSSRFFVPPGSVKPAHCIFEHVYFAKQSSIIFGENVHAVRVRCGRR
                     LAIEHPVDADVVIPVPDSGTSAAVGYALQSGIPFDMGFVRSHYIRRTFISPSQKMRDL
                     AVKLKLAVVRDVVKGKRVVVVDDSIVRGTTTKGKIQSLRNAGAKEIHMRVACPPVKNP
                     CFYGVDFPTREELLANQVASIEEIRRFLQVDTLGYISLEGLLGCASLPPDHYCHACWS
                     GQYPIPVSLVVNKFSMERHQMQLFDDIEL"
     gene            14100..15176
                     /gene="purM"
                     /locus_tag="PLE88_05105"
     CDS             14100..15176
                     /gene="purM"
                     /locus_tag="PLE88_05105"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOM75880.1"
                     /translation="MRKKTFISYEESGVSIDANDRMVERISRSVVSTHGPRVINLENG
                     FAGLFRLDYDEKLFKRNYRNPVLVACTDGVGTKVLIAQQMGRFDTVGIDLVAMSVNDM
                     IVQGAEPLLFLDYLGVNKLEPEIIAQMVESIAAGCRMADCALIGGETAEMPDIYDKKG
                     EYDMAGFAVGVVERDRIITGSDVRKGDVVLGLASSGLHSNGFTLVRHICFKKNKLKTA
                     DRIDALDGARLGDILLEPTRIYVRSIVNLLHSYKVKKVVHGMAHITGGGLVGNIPRVL
                     PADCNAVLKKNAWPVPKIFRYLQKIGPVEEYEMYRVFNMGIGFVLIVAADFADAIAAK
                     LTKSGEQVYRIGMITAGSRKVVLK"
     gene            15246..16289
                     /locus_tag="PLE88_05110"
     CDS             15246..16289
                     /locus_tag="PLE88_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75881.1"
                     /translation="MGKTSANRSSRSALAAGKHSLPALILYSILAIYLFYPYINRFST
                     VQFLYVINPIAAALGAYILSRRWLYHPAASVTAGAAYGFGPFGLGFTGFHPLAGFSWM
                     MLPWLLLPAVYWRQGQTPDGLRFGGRAAFSLLPFVWMVLLFRIAAQPWIGPFALLPLT
                     ASLTLEDFKGLIFPLHEAGQHIVFGLYPTAFVMALMGLFVYAKLQRIGPLIPVAVGTV
                     LAFCGPVFQVSPVVWAAFPVLFLSLLSGLGIEALSAAGKADSKWVAVCAIFATVLSAF
                     FGGLSIGIVLISPEVFKLTALLYGLAAAALWLLLCLIRLQLHWPLARWALLTCAAAID
                     LFLASRYFTAKLF"
     gene            complement(16340..17095)
                     /locus_tag="PLE88_05115"
     CDS             complement(16340..17095)
                     /locus_tag="PLE88_05115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOM75882.1"
                     /translation="MEQQVNWDKIFLQHLEMEAFFVGDFTVKRPQRAQSDVLSGAVCA
                     KTADEEFLTDDSLEKIAAEISACRKCEIGRSRINPVAGQGNPKARLMFVGEGPGAEED
                     QQGLAFVGRAGKLLTDIITAMGLKRQDVFIANIIKCRPPGNRDPKAEEIINCWPFLKR
                     QIALIRPQVIVALGAPAARTLLNTNESIGKLRGRFFEYRVSDEAPPVKLMPTYHPAYL
                     LRNYSYENRKRVWEDMIKVLSELGLPVPQKKKD"
     gene            complement(17176..19794)
                     /locus_tag="PLE88_05120"
     CDS             complement(17176..19794)
                     /locus_tag="PLE88_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOM75883.1"
                     /translation="MERFPYPSTGLNHLDELLTRLQYGDNVVWQVDAIERFKELAAPF
                     AARAVAEHRKLVYFRFAQHLPLLEPQPGLTIVELDADTGFETFTTQVHQIISQQGRGA
                     FYVFDSLSDLLTTWATDLMVGNFFTVTCPYLYELDTIAYFALLRDYHSFQTVARIRGT
                     TQLLLDVYHLEGTRYIHPLKVWNRYSPTMFLPHRDDGTQFTPVTDSVDAARLFSYISD
                     IARSRTRRKLDYWDRLFLNAETLVSRYADTDKDNPEYKQAVETICRIMMGREERMLAL
                     LKSHFTLRDLLDIKARLIGTGYIGGKSVGMLLARKILSQDASFDWQSRLEPHDSFYIG
                     SDIFHTYIVQNGLWKLRMEQKTPEGYFAKAPELQSGLLKGKFSSDIREQFQEMLEYFG
                     QSPIIVRSSSLLEDSFGNAFAGKYESLFLVNQGDPDERYAKFEEAVRTIYASTVNEDA
                     LTYRLQRGLDQMDEQMALLVQRVSGSPHGSYFYPDAAGVGVSCNTFVWHKDLDPQAGL
                     LRLVAGLGTRAVNRVDDDYPRLVALDAPSLRPPVSAGNQRQFSQHYIDVLNIADNCEQ
                     TLPIQQYLASNPDWDFHLLAAEDQEAADRMRQLGIDGPRQYIMTFDLLLEHTDLAAVM
                     RRLLKTLEQFYDYPVDIEFTVNLAGNLPPQINLLQCRPLQASGVGSRIRLTERLDPER
                     ILFRTQGSFMGGSISRTVNKIIYVHPSAYIALTQQEKYELARLIGKLNRQFCSRKSDF
                     TMLMGPGRWGTSTPSLGIPVRFSEINQVTVLCEISYPGGNLMPELSYGSHFFMDLVEE
                     RIFYIALFCEKSDVLFQPGLLLKRPNLLTRLVPEAEKFSQTVFVYEITDKSLRLEADV
                     FSQKLVCYFEAPKTSK"
     gene            complement(19934..21274)
                     /gene="gdhA"
                     /locus_tag="PLE88_05125"
     CDS             complement(19934..21274)
                     /gene="gdhA"
                     /locus_tag="PLE88_05125"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOM75884.1"
                     /translation="MATQFVDSIMEQVTARNPGEVEFHQAVREVAESVEVVIKKNPEF
                     AKAKVFERIVEPERQLMFRVAWVDDKGTVQVNRGFRVQFNSAIGPYKGGIRFHPSVYL
                     GIIKFLGFEQIFKNALTTTPIGGGKGGSDFDPKGKSDNEVMRFCQAFMNELFRHIGAD
                     TDVPAGDIGVGGREVGYMFGQYKKLANVFTGVLTGKGLTYGGSLVRTEATGYGLVYFM
                     TRMLQDRGTDWKGKTVVSSGSGNVSIYAVQKAQQLGARVIAMSDSNGYIVDEAGINLA
                     TVKRIKEVERGRIKDYVNAHPKAKYVEGCQGIWTVPCDIAMPNATQNELDKQAAETLV
                     KNKCIAVGEGANMPSTPEAIEVFLNSGVSFGPAKAANAGGVATSALEMCQNSMRLNWT
                     FEEVDNRLRSIMNAIYDQCIGAAEAYAAKGNLVAGANIAGFLKVAQAMMAQGIV"
     gene            21531..22409
                     /locus_tag="PLE88_05130"
     CDS             21531..22409
                     /locus_tag="PLE88_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOM75885.1"
                     /translation="MAKIFCDLAELKNFSRTAELHGISQSAVSQQLAHLEMVHNTQFI
                     NRKERPLELTASGQAFYRACKDILERYNQFNHELASLTKTTCRVNIASIFSIGMHTLQ
                     PYIKKFIRNYPDVNLVVEYLDAAEIYTRLLKGSIDMGVVALARHDRATQVYPFENEPL
                     VVVCALGHPLAEAKEIDIHQLQGSKFIAFSKNVPTRQHIDSILKRYGVAVQIAMEFDN
                     IETIKRAIEINSGISILPKPCVQTEVTSRTLVELNLSNEHFFRPTGIIVRKDHQLNKA
                     AQALLDLMRKDTSIEL"
     gene            22423..23250
                     /locus_tag="PLE88_05135"
     CDS             22423..23250
                     /locus_tag="PLE88_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75886.1"
                     /translation="MIKLKSMQILIIGLCTVLNIWASDVCPNADKTDSYNSQTADSGA
                     GDLEQILSRIVESTRQLKSCQARLSYLFVQDPDLLASRTLRTGTLYYINGGEKSLLRI
                     HFQDIKQDDFEPEARREEYLFDGVWLTRIDFKLKQVDQFQQAPEDKPMDVFELISHNF
                     PIVGFSGTDKLQQDFNIALKEKNNDPNEPICLLLEVKQGSKFHEEYEKIDFWIDPANY
                     LPKRLLAYAVQGDLYDISFSDIEINKKLEKSVFTIETPADFRKNVEPLKNSGDRKGT"
     gene            23254..23730
                     /locus_tag="PLE88_05140"
     CDS             23254..23730
                     /locus_tag="PLE88_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOM75887.1"
                     /translation="MAKGLVLYYSRSGNTKMMANAIAEAMEKAGLPTKCKSVTDAKVS
                     DLVDADAVVVGSPTYYGRAAAAVAQLFDESVSKHGKLDGKVGAAFSSSANIGGGNETT
                     ICEIINMMLIHGMIVQGDPQGDHYGPVSIGKPDERVLTQCARRGQRVAALTLKLFG"
     gene            23829..24002
                     /locus_tag="PLE88_05145"
     CDS             23829..24002
                     /locus_tag="PLE88_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75888.1"
                     /translation="MQAYEELKQLVAEIEADINKAEGGNKAAGTRVRKQMQRIKQAAQ
                     NVRNNILEIRSSE"
     gene            24114..24587
                     /locus_tag="PLE88_05150"
     CDS             24114..24587
                     /locus_tag="PLE88_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75889.1"
                     /translation="MPPALLVDLSTIDLNTVEYDRSAIAEVNPQSYEMSQLDGIIWHD
                     LERMLCVGFKDVTEHEFWVRGHIPGRPIMPGVIMVEAAAQLCSFFMKRIYGLTGFIGF
                     AGIDNTKFRETVPPGKRLYLLGHIHKVRSRQFSAMVQGVVDGKMVFDTVISGMRV"
     gene            complement(24653..24725)
                     /locus_tag="PLE88_05155"
     tRNA            complement(24653..24725)
                     /locus_tag="PLE88_05155"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24689..24691),aa:Glu,seq:ttc)
     gene            complement(24735..24908)
                     /locus_tag="PLE88_05160"
     CDS             complement(24735..24908)
                     /locus_tag="PLE88_05160"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM75890.1"
                     /translation="MPAKVDPGTCTGCGSCVDACPSEAIEMQDGKACVDADKCVDCGV
                     CVDECPVEAIRME"
     gene            complement(25100..>26371)
                     /locus_tag="PLE88_05165"
     CDS             complement(25100..>26371)
                     /locus_tag="PLE88_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75891.1"
                     /translation="AWSNRAFEEAPQRQGVSVMFAYALAVHGQYDLAAQYAQPLRQTD
                     QIAALTMALVELAADQKQAALDSLRTAVEMAPNTFAADKAIRLIKDQGSDYIPSVSLE
                     TIQKDLQQQYGKRVVPAFNVPADHYALRLLFNGSDFHYGMEISPRLIIENTGTEPLII
                     ADGSCLSGVIRIDAVLKGDLNVEIPNLLTTKVRPSRPIQPKSYVSVPLDLNTGKLRKL
                     LAAYPQADVEVHFTVYLDPVLSPDGRVENRLQGLNPVRAAIQRPGVVLSRDFLIQRLE
                     ALSKGQPGQKLQAASLFAGLLAEQKAFELSQADFRHIQTERMLLVDAVRRSLADDDWK
                     VRIQTMASLLDGSVPMEYGLIREISANLHHDLWPVRFMAMYLLASAQPGTFQKVIDWA
                     AQYDTYPLNRRLAAALGANTGPQTQTDPNSL"
BASE COUNT         6919 a         6913 c         6328 g         6191 t
ORIGIN      
        1 ttcaaaaatg tccacaaaag aataacactt ttaaaggatt tgtccatttc caactaaagc
       61 tttacactgg acgctggtgt gacgctgggg aatttataga gttgaaaagt agtgaaaaaa
      121 tgttgttttt ggtagtaaaa ccactgcggg tgacgtgaat cgaacacgcg tgtccagctt
      181 gggaagctga cgttctacca ctgaactaca cccgccaaaa cattcttgca ggcatatata
      241 tatctttaca aaggctattg tcaagaaaaa ctttatataa gctaagttca agacttcaga
      301 tatccctcaa taatacaggt ttttttgatt ctttgctttt ttctggtatt ttgggcttta
      361 atccggcaga tcagtcaaca gccctctctg gatgcaaaaa aactctgcag aaacaatgaa
      421 tccattgttt ctgcagagct cacttatctg caaaaggcct tttcagccgc tgtgatgtca
      481 gtttgaaagc tgatatccgg ttgtaatatt attttgttga aaacttatgg acagtagagg
      541 tcttgtaaac ttctcccggc cgcagcacaa ccgatgggaa ctgggcttta ttgggagaat
      601 ccggatagtg ctgcgtttct aaacagaaag catagcgatg cttataaacg cggccttcct
      661 tgcctgtcag cgtgccatcc aagaagttgc ccgaatagaa ctgaataccc ggttcgttgg
      721 tgtaaatctc cattaagcgg cctgttgacg gctcatatac acgggcggcc agcgtcatct
      781 tgctgccttt tttattcagc acccaattgt ggtcgtagcc gccgccgtac ttaatctgct
      841 ggtcatcagc attaatgcca gcaccaatcc gtgtcggctt ggtaaagtcc ataggcgttc
      901 ccttgaccga aacaatctgg ccggttgtaa tcagaccttt gtcaacagga gtgtaattgt
      961 cggcattgag catcaattca tgaccgagta tgtcaccctt gccctgccct gcaaggttaa
     1021 aatagctgtg attggtcaga ttgcagacag ttgccttgtc ggttgttgct acataatcaa
     1081 ttttcagttc atttttattg gtcagggtat aggtcactgt tacatccaga ttgcccggat
     1141 agccctcttc catatccttg ctgaggtaat gaagcttcag ccctacactc ttggcattct
     1201 taattggctg ggcttcccag acaactttat cgaagccctt caggccgccg tggagatgat
     1261 tttcgccgtc attggttgca agctgatatt tcacgccgtc cagcgtaaac tggcctttgc
     1321 cgatgcggtt gccgtaacgt ccgataatgc agccaaaata agggcttttt tgttcgtaat
     1381 ccttgatatt atcaaatccc agcacaatat catccagttt gccgctgcga tccggaacac
     1441 gaagcgagac tacagttcct ccataatttg taatcttgac ctccatcccg ttggtatttg
     1501 tcaaggtgta gatatcaact ttcttgccat cgctggttgt accgaaatcg gcttttgtaa
     1561 tatccaattt ggccgccgcc gttttagccg aaggacccgc gcatccagct gtcaaaaaaa
     1621 cagataccac cagcaaaacc gccgcacctt tcagaattgt ccgcattgca atacctcctg
     1681 tgctaaagtt tctctctgtt caaccaaacg cataagcgtg attatagtag tcctgccccc
     1741 tctgtcaact ctcaattgca ggttttatgg atgttttaca gcaagaacta attgagttca
     1801 gggcagcccg agggatttaa catggccgat ttggcatttt caacaatgca ttagtccttg
     1861 ccgttttgcg gttgacggcg gcccagaaat gtaatcaaac catccagaat tgtataagga
     1921 tgaggcggac agccggggat atataaatcc acaggaataa gagcatccac accgccgcaa
     1981 atttcagaat ggccgctgta agggcctccg gctatagcgc agctgccgac tgcaattaca
     2041 aacttcggcg aaggcactgc ggcataagtt tcttccaatg catagcgcat attctgcgta
     2101 accggcccgg taacaagaat tccatcggca tggcgcggcg aggcgacaaa ctgtatcccg
     2161 aatcgtccca aatcaaagac cagcgttgta agcacattgg tatcggcctc acaggcattg
     2221 catccgccgg cactgacctg acggagcttg agagaccgac cgaagattcg ccggcattgc
     2281 tgattcatcg cttccgccag tttcggctca atatctgaaa ccaataaatc ctctcgtcga
     2341 ttgaccgcaa gccggtaatc gccggtaaac acaatggcgc tgcgggtgca tgctttggcg
     2401 caatccccgc aaaaaaggca cttgcccaga tcgatgcgaa tccggtcatt tacacaatca
     2461 atcgcattca caggacaggt cttctgacaa gcaccgcact gattgcagag attgggatgc
     2521 aggcggggtc ttccgcaaaa ccgctccggc agaaccgggg gcttatccgg aaacggatag
     2581 gttcggtaac cctgtttgat tcgagcaatg agcgaatgaa tcatactgtc ctcacaggtc
     2641 atgtccgcag taagaaaggt tgaagctctt gttgcacagc gggaaatccg aaatctgctg
     2701 gccgcgcatc gccacagcca gacccatcca attatgaaaa gacgggtccg ctattttgta
     2761 ccgaacaaat ttccccgtgc tgtcggttac agctgcatgg cagatttccc cccgccatcc
     2821 ttcggtcaac gctacggcga ttttgttggg cattctgtca gcaagagact gatgtactga
     2881 tccggcaggc atcgtgtcca gctgattcag aataaaagcg gcactgtgct ggatttccag
     2941 ccagcggacg taggcccgtg caaagacatc accggtattc catgccgcta ccggcatctg
     3001 gtgaaactgg taaatgccgc tggcgaaatt ataccggcaa tcccgatcca gtccgcaggc
     3061 gcgggctgcc ggacctacca gccccaattc cacagctgtt tggcgcggca gagtaccggt
     3121 tccatcaaat cggctcaata ccgacggtgt tgaccagaga caatcgactg cacaggccgt
     3181 atccttaaca gtagcggtca aacggcttcg caggtcgtca atatgccgct gttccaaatc
     3241 aaaaccaact ccgccgggac atacaaaatt ccggccgaaa cggtttccgc acagcagcgc
     3301 agtcagattg agaaaatctc cccggattcg cccgcagaag gaagccgtag gcagataccc
     3361 gacatcccct gccaatgctc ccaaatcgcc ggtgtgattg gccagccgtt ccagttccag
     3421 tgctataccg cgaagcacat gagcccgaat cggcaccgaa cagcagctca gcgcttcgat
     3481 cacatggcaa tacgtcaagg tatggccgac ggttgtatct cccgctgccg tctgcatata
     3541 atgaattgac cgtttgtccg gggcaccgat caggcgggat tcaatgccgc ggtgctggta
     3601 tcccaatgcg atttctaaat gcagaacatt ttcgccgttg cactgaaaac ggaaatgccc
     3661 cggctcgata atgcccgcat gaaccggtcc gacggccact tcgtgaatct cgctgccttc
     3721 gacacggaaa aaatctgtta cgcaaggaag aatcggtgca tccgccggtc gggcccaggc
     3781 atctcgtccg gtgtaagact tatgatagcg aatcggcttc agccaagggt gtccttgcgg
     3841 gcgcacacca aactgctcgg caatttcccg ctcaaaccag tgggcttgcg ggcagtcagg
     3901 cgtcagtgca ggataactgt ctttgacaag ggcagatgcc aaatgcagca gcccccggcg
     3961 nnnnnnnnnn cagtttcact gcctctttcg catccggcat gccgaacaat aaagcgatac
     4021 gcatattttc gccgacgcgg cggataataa agtcccgaaa ttcctctatc ggcaaaaccg
     4081 gcactgcatc gatatcggca gttttacaat tgtataagga aagcatctgt gtatccatta
     4141 tcgcccccct aatagaacgg cggcccgatg gagcatatca ttcaccggct caggcacata
     4201 aacacccaga agcagcaccg ccgttatcaa caccagcggg ggcacaaccg ccggcggccg
     4261 ctcgcaggca ggctgaaccg attcgggttt ttgcccctgt gtcatccgca aggcaattgc
     4321 cgaaaatcca ataaaaataa aacccagcag tatcagaaaa gcagcagcgg ttccataata
     4381 tccctgctcg acagccgatt gtagtatagt aaactcgctt agaaaagtcc caaagggagg
     4441 aaaaccggta attgccagca aacccgccac ccacagcagg cccgatacgg gcatcacaga
     4501 aaacaatcct ctcacccggc ttgcagtttt ggtcttataa acagccaaaa tattgccggc
     4561 cgtcataaag aggaccgcct tggtcagcga atgattgact gcatgcagca tggctccgga
     4621 aaccgcagct tgacctaatc ctgtacccag cgccagaatc cccatatgct cgacgctgga
     4681 ataggccagc agccgcttgt aatcggcctg cccgataatg aacaccgcag caaatgctat
     4741 cgacacaagc ccaaacagca ccagcaagtc ctgtgcaaag gccgtctggc cggcagcaca
     4801 ggctacctca aaagcccgta aaatcccgac aaacgcacaa ttgagcagcg cccccgacag
     4861 cagtgatgaa acaactgacg gcgcttcgga gtgagcatcg ggcagccacg tatgcagcgg
     4921 ggccagtccc attttggttc catagccgac cagaaagaag ataaatgcgg cctgaatcca
     4981 cggggttgac agtttcgaac cgttggcaat caaggcattc cgcaggagac taatcgattc
     5041 ggtctgcgtt cccgaagccg caatcgccag aaaaaagttg cccagcagcg ccagcgctat
     5101 gccgacagag caaatcagca gatatttcca agtcgcttcc agcgaacggt ggtggcgatg
     5161 aaaatagatc agcggagccg aaaccagcgt agttgcttcc atagctaccc ataacaggcc
     5221 gaaatgctga cttgcaagaa ccattgtcat cgttgttaaa aacagcagaa ggcagccgat
     5281 aaatacggcc tgcggggcgt ttatgaaaaa cgcatcttcc ataaaatcct ttttccggcc
     5341 ggtttgctcg gccttcaaat agccgacggc ataaaacgcc cctgccagaa agagcacact
     5401 ggtcagagtt aaaaaatact gggcaatggg gtctatgtca aaccacccgc ccagaatcgg
     5461 cccggtcgga taaacccagc aagccgccgc cgctgccgta tggcacagcg ctgcagcaat
     5521 cagcagcatc cggcgaagag cgtcggaccg aatgagaaag ctggccacag ccgctgccag
     5581 cgggacaaat atcagcatcc aaagcatcag tgatccctca gcatcgttaa ttgatccgta
     5641 tcgatatggt caaactgccg gctgatatga aaaatagcaa ttcccatcac aaaaacaccg
     5701 acaaatacat ccagcagtat ccccagttca atcagcagcg gctgatgata cgccaccgcc
     5761 atcccgaagg cataaatgcc gttttccaga accagatagc ccagcacctg cgtgagggca
     5821 tttttccggc tgacgatgac atacagcccg ttcatcaggg taaacaaagc aaccggaatg
     5881 agcatcgggg aatgcacgct gtacggcaga ggcaagcggg gactaatcca gcttgaaaaa
     5941 gccagcagga gagggcccac caaaagagaa agcccatagc cgacatacgg ggcatcctcg
     6001 cgccagacat tcagctgccg cagggtacgc ccgagcatca tcggaaaaat gatgcctttg
     6061 agaatgatgc tcaagaatgc aaacaccagt aaacccggct caaagcggtg aatcagcaga
     6121 ggcagaatcc ccagcgcaac gccctgtaca gccacaatcc ggatgcaggc acgcagccgg
     6181 ctggttccca gcaccgccat attggtcaaa atcagaatca ccatgctgat atcagtccac
     6241 acggcttagc tcctcaatgc aaacaccagt gccagtattg ccagagcagc tgcggtaaaa
     6301 agcagatgcg gcacacgcag cagacgcagc cgtgccatac tggattcaac aataccaatg
     6361 aaaactgccg tcagaaaaat tgcggctgcc gaaacagccg cagcggccag cggcggcagt
     6421 gtgccaaggg gaagaatcgc acccgtcagc agcgctgcat aaagccacag cttcagggca
     6481 gaaccgtaaa gaataaatgc aaaatccggt ccgctgtaat ccaagaccat tacctcatgt
     6541 atcattgtca attccaggtg cgtgttgggg tcatctaccg ggatgcgggc attttcagcc
     6601 agcagcacta tggccagtgc agcagaaacc agcagcaacg ttgccgaatg agccgtccag
     6661 aaatccggcg taatggatga aaagatgccc gacagagaga ggcttgatgt ctggcgtgcc
     6721 agagccatca gcgctatgaa taaagccggt tctgccagcg ccgaaaaatg cacttcgcgg
     6781 ctggctccca ttccctcaaa actggagccg gtatccaagg cccccagaac aacaaagaaa
     6841 cgcgccaagc cgaataaata agcagccaaa atcaaatcgc cgctgaaact aaacagactc
     6901 cgcacgcccc ccagcggaat gatgcaggca gccgtcaaaa acgctgccag actgactacc
     6961 ggcgccgcgc ggaaaatcca cgaggcagcg gtgctgtaca ccgctccttt atggagcagc
     7021 cgccacaggt cataatacag ctggaacaaa ggctgcccgc gccggccggc aaaaaacgcc
     7081 ttgacacggt tgattacacc cagcagcaaa ggagcaagag tgatcgcaag aagcggctga
     7141 attatcattg cagtctgcat catcggagct tccaaatcag aagaatcatc agcgtaagtg
     7201 caatatacag tacatacacc tgcacgatac catactgcac ccagcgcatt ttaccaataa
     7261 accaatccac ccatttaaaa atgggtcggt acagatactc gctgctgaca tcgggtgtat
     7321 gggtttcaaa ttccgcctgc cgcggaaaaa actctgtaat acgcacaacc ttgcgccggc
     7381 tgccgagaac agctctgaac atatccacaa tcggctgagc aaatgaggaa cctgtatact
     7441 gcatccgcga tgtcggggcc gcaaagccgc agccccaagt ctgctcttct gccgttgtcc
     7501 ggccgcgcag aattttccac cgcaaaagcg ctgccccagc cgccagcatc agcagcagta
     7561 tggacgctgc agcaatacta tacaacattg ggcgggcggc gggaatcggg tcatctgctg
     7621 cctttgaaaa acccgcgctg ctgacgacac aggcaatgga tgaaaacagc catggagcgg
     7681 ctgcactggc catcacacag agcaatgcga gtactcccat cggggctgtc attgacagcg
     7741 gcgattcatg ggccacctga gcccgctgcg aacgcggcag acctaaaaat acaattccaa
     7801 agactttggc aaatgccgct aaagcaaaac cgccgatggc tgccaaagcg gcaattgctg
     7861 ccgctgccgg cacgacgatt tcaattcttt ccgaaaggac ggccttaaat gagccataat
     7921 ataaaaggaa ctcgctgata aatccgttca gcggcggcaa ggcgcagatg gctgccgcac
     7981 ccaaggcaaa tccagtcgct gtaaacggca tctttttaat caggccgccc agcacatcga
     8041 tcaaacgggt accggttgca tgaacaacgc tgccggctcc cataaacagc aggctcttaa
     8101 aaagggcatg attgacggta tgcagcagtg ctgcgctgaa tcccaaaacc gtcagtgccg
     8161 gcgactgccc tgccataccg acaattccca accccaagcc gattaaaata atgccgatat
     8221 tttctatgct tgaatacgcc agcatccgtt tgatatcgtg ctgagccgcc gcaaacagaa
     8281 tgcccagaac tccagagagg attcccagac ccgtcaacag ccagccccat tcaatctgaa
     8341 catcagggag tactgaaatt gttcttacaa taccgtaaat gcccatcttg agcataaccc
     8401 cagacataac cgctgaaaca ggactgggcg cggccggatg ggcttccggc agccatacat
     8461 gcagaggcat caagcccgct tttgtgccaa aaccaaccaa ggccagcaga aagagaatag
     8521 cggcctgtct atctccaaca tccaccccag caaaatccca attgttattg tatttgccaa
     8581 gcagtacaaa aaaggccagc agaaatgctg ttccgatatg agcagcaatc agataaaccc
     8641 agccggcctg tcgaacagac cggtgttcat cctcaaatgt aaccagaaaa taggacgcca
     8701 gcgtcattac ttcccaagac acgagaaata aaatgctgtt gcgcgccagt acaaccattg
     8761 ccatagcagc cgtcagcaag ttaaagaaaa acgccggtac ccccagacgg cggtccttat
     8821 aggccgccat atagccgcag ccgtacaccg cggcaaccga acagactaca aaaaggggca
     8881 ttaaaaacag agatgaaagt ctatccagtt cgatatggaa tgccccatat ggtacttgcc
     8941 aaggcaaatc cagcgacaga attgtaccgc tcagcaggac ataccctgca ccatacagcc
     9001 ccagcaacga accggcgagg acacctgcag cgccgattcg ggtacagatt ctcccattgc
     9061 ggataaatag acagagaaaa cccgatgccg caattaaacc aatcccatat aatatgaaag
     9121 ccatttactt cttctccttc tgttggtctg gcagcggcga ctggggggca tcaaggtccg
     9181 cttcaatacg cccgatatgt gaaaaaatct catcgcggct tgcctgtatc tcaatagcat
     9241 gcttaagagc tgcatcccgc agggcataac tcagacgcga caaaaggtgc aggtgtgccc
     9301 gaacagtagg gcttacaatt gtaaagaggc aatagactgg ccggccgtca atggccttaa
     9361 aatcaatcgg cttctcgaga aaacacagag aaaccgtcgg ctgaggcaca tggagtacaa
     9421 tcggattgcg gacatgggga atcgcaattc catcgcctat gccggtcgag gccatttcct
     9481 cacgggcaag caatacattg agcagaaact gtctgtccac ttccggcggc agatgaatca
     9541 attcaacaac cgaccgcagt gccgatgcct tgtctgtccc gccgacccga tagtatatgc
     9601 cccctgcccg cagcgcctgt ccaagcccct gcaaaggaga atccgcagat tccggctcgc
     9661 tgaacaattc tgcagagacg tttattcggt tggccatcgc ccattcgagc agttctgcgc
     9721 ggttaaatcg gtacatattg ttgacacgat agaccggcaa tgtcttctga tcaagccagc
     9781 gatatactgt tttttcactt accgcaaata agcgggccac atctcgtact gtcaattgca
     9841 tagaaatttg tctctcttaa tggacatatt tggacattta taaacatatg tttaaagaag
     9901 tcaatagcat aaaaaatttt ttctgtatac tgactgactt ttttaaaaat acatttatga
     9961 cgcgggggat gtttatacag cgtgtcgatt tcttaactta tattaacaat tataattata
    10021 tcgctcaaca gcaatataaa cagcaagccc taagcctgca gcagttgcca gcaccagcag
    10081 actggctggc ctaaatgccc aagcgtaaga aacagaattg taaatcagaa aggcacaaac
    10141 aatacaaaaa atcaggggcg gcaggggata gaatgagact tggtacggac ggggtacctg
    10201 cggctgcctg tatcggagta caatcactga aaatgctgta gccatgtaga aagcatagac
    10261 cgccgcagct gtgtacaaaa tcgcatccat gtagccgccg aataccacta tgaggcacac
    10321 ggctatcgat ccctgcactg ccaatgccca aacaggtcct cctgtatgct gattccaatt
    10381 gcccagcagg cggaacaatc gatactcact tcccatggcg tatgaaatcc gggcacccgt
    10441 aaatatcaga ccgtttacag cgccaagtgc cgaaacacat atcagggcac taatcagctg
    10501 ccggcctcca cttggaagca aacaggcaag catatcactg gccggcgctt ccgatgcagc
    10561 cagcccccca aaagtcaatc catacacaaa tgccccatta accagcagat acagaatcat
    10621 cacggccgcc atcccgactg ccaatgctcg gacaatattt ttgccgggat tcttgacctc
    10681 tgctgccaca tatgccacct cgttccatcc gccataggta aacagaacaa aaatcagcgc
    10741 caaaccagcc ggaatcggct caagcggcct tgccggctcg acaggccggt tcactgctaa
    10801 tccggcagcg gcgattccta tcaatcctac agcttttacg gccgtcagca gattttgcgt
    10861 ccatttaccc tgccggactc cgagcatatt catcagcgtc aatacaacaa ctgcccctgc
    10921 ggcgtaaagc cgctggctta tctggggatc aaccgccgcc ggttcccaaa tctgccgtcc
    10981 gtatgtcgca aaggcaaaag ccatcacggc tatgtcgccg gggcggacaa taaccagctg
    11041 cgaccatccg aataaaaaac cagccggtcg gccgtatgcc tttgttaaat agacaaaatc
    11101 accgccggtg ctgttcggat aggcagccgc cagttccgca taacttagtg ccccgcataa
    11161 ggacagcagc cccccgataa tccataaact catcagctgt gcaaccgtgc cggccccgcg
    11221 tgcaacagcc ggggttatct gataaatacc cgctccaata atgatgcctg ctgcgataca
    11281 gactgcatca aataaagata aaatcctctg gggagcagct tgcatgcttg ttttccagac
    11341 tgcctgctta ctattcgatg aaccgcagga gtttatcttt tcttaagcgt taatgacacc
    11401 ccggaccgca caattgtttc caattccgga tttcccgtga cggcatcaat aaaccgcata
    11461 tcggccatgc ccggtgtgat attgtgagca ataacaagcc cgccaggacg caccaaaggc
    11521 agtaactgat tcagatagtc gatgtatccg tccttatctg catccaaaaa aattatatct
    11581 atcggctctt ttagcatttg caccgttttg tgggcgtctc cctcgataac agtaatacaa
    11641 tcatcaagcc cggctcgctt aaaattggct cgtgccgcag ctgcccggcg ctggtctatt
    11701 tcaaaggtcg ttaatttccc gccggttttc tgcagcgcca ttccaaacca gacagccgaa
    11761 tagccggtag aggtcccgat ttccacaata tttttggcat tggcaaattc tgcaagcagg
    11821 cgaagaaggc gtccatcgct ttgagggaca ttcatagccc gttgattttg gagaatatct
    11881 tccagaacag caaggattgt cttttcgttt tcattcttgg ccaaagggtc tttttgaaat
    11941 tgaatatctt gctgattttg gctgattctc cgtcccgctc ccggctgtct gtctggccca
    12001 gtcgcttgta tagccggcct gaatatctgc cggttttgtc gggccagact gatcagctga
    12061 tctatctttt ccagattatt atctcgccgg gcctgctcca aatcattcag ccagcgttcc
    12121 aaatctgccc cggcaccttc cccgcccggc cggactggtt gtacttcggg tccagcagcc
    12181 aacataaaac acaccatacc cgccgcaatc cccgctgcaa ttacaatctt tctcatacat
    12241 tgtcctttcg ttattgattc cgactggatt gacactactg gatttggata atactgctcc
    12301 ttatttctga tttacttgta tttggttatt ataatgcaga aaggatataa attgtatatc
    12361 gaaatccctg acnnnnnnnn nncatcccat tgaactcttc atcttccctg agacgctgaa
    12421 atattgaatt agatgtggat atgggagaga gaatatctat cctatcttac attgtcgctt
    12481 ctgccctgca acatgccgat agagcagatt gacaaactgc gcatactcgg ttcatttttt
    12541 tgtccctgca gtcttgcaac gaatctgccg ctgaacaaaa agtaaaatca ttgacttatc
    12601 gcagcatatc ggttaacttt tatttttaag ttgtttttca tcgattttac gaggactcaa
    12661 tggcagataa aaaagaagcg tgcggcattt tcggtatttt tggcgacccg gacgccgttc
    12721 ataaaacata ttttgctctt cacagtttac agcatcgcgg acaggaatcc gccggcatag
    12781 ccagcagcga tggcgaactt atccattgct atacggggat ggggcaggtc agccgtgtct
    12841 tccgggaggg ccggggcatt cttgagcggc tggataatcc gattgctatc gggcatgttc
    12901 ggtattctac atccggatct gccaatatcg ccaacgccca gccgttttta agcgaatatt
    12961 ctcgcggaca agtggcggta gcccataacg gcaatctgat aaacgcctcc ctgcttcggg
    13021 atgaatacga ggcctatggt catatcttca agagttccaa cgatacagaa attattgtcc
    13081 atttgatggc caaacctact catgttacca aaccggaccc gctgggacat gtccttaacc
    13141 acctgcaggg ttcgtattca ctggtttttc tttttccgga ccgtatcgaa gcggctcgag
    13201 atccgtatgg catccgccct ttgtgtttgg gacgtaccgc cgacggcttt tactgcgttg
    13261 ccagcgaaac gtgtgctctg gatgctattg gggctgacta tgtacgggat gtcgagcccg
    13321 gcgaaatcgt cacgctggac aaacacggac tgagcagccg cttttttgta ccgcccggtt
    13381 cagtcaaacc ggcccactgc atttttgagc atgtgtactt tgccaagcag agcagcatta
    13441 tttttgggga aaatgtgcac gcggtacgtg tacgatgcgg gcgccggctg gcgattgaac
    13501 atccggtcga tgcggatgtc gtcattcctg taccagattc gggcaccagt gccgccgttg
    13561 ggtatgccct gcaaagcgga ataccgtttg atatgggatt cgtgcgaagc cactatataa
    13621 gacgcacctt tatttcaccg tcccaaaaga tgcgtgatct ggccgtaaag ctcaaactgg
    13681 ccgtcgtgcg ggatgtggtc aagggcaagc gggtggttgt tgtcgacgac tcgattgtcc
    13741 gcggcaccac caccaagggc aaaatccaat cccttcgcaa tgccggcgct aaggaaatcc
    13801 atatgcgggt ggcctgtccg ccagttaaaa atccctgctt ttatggagtt gattttccaa
    13861 cccgggaaga actgctagct aatcaggttg ccagcattga agaaatacgc cggtttctgc
    13921 aggttgacac actaggctat atttcgctgg aaggcctgct gggctgtgct tcgctgccgc
    13981 cggaccatta ttgtcatgca tgctggagcg gtcagtatcc gattccggtc agtttggttg
    14041 taaataaatt ttcaatggaa cgtcatcaaa tgcagctttt tgacgatatc gagctataaa
    14101 tgagaaaaaa gacattcatc agttacgaag agtcgggagt cagtatcgac gccaatgacc
    14161 ggatggtcga acgcatcagc cggtcagtcg tttccactca tggaccgcgt gtgatcaatc
    14221 ttgaaaacgg ttttgccggt ctgttccgtt tggattatga tgaaaagctt tttaagcgaa
    14281 attatcgcaa tccggtgctg gtggcctgta ccgacggcgt cggcaccaag gtattgatcg
    14341 cccagcagat gggacgattt gacaccgttg gcatcgactt ggtggcgatg agcgtcaatg
    14401 atatgattgt tcaaggcgcc gagccgctgc tgtttctgga ctatctgggt gtaaacaagc
    14461 tcgaaccgga aattatcgcc cagatggtag aaagcattgc cgccggctgc cgaatggccg
    14521 attgcgctct tatcggcggc gaaacggcgg aaatgcccga tatttacgat aagaaagggg
    14581 aatatgatat ggccggcttt gctgtcggtg ttgtggagcg cgacaggatt atcaccggca
    14641 gcgatgtcag aaaaggcgat gtcgtgctcg gattggcttc cagcgggctg cacagcaacg
    14701 gctttacgct ggtacgtcat atctgcttta agaaaaataa actgaaaaca gccgaccgga
    14761 ttgatgcctt ggatggcgcc cgactgggcg acattctgct ggaaccgacg cgaatttatg
    14821 tccgttcgat cgttaatctg ctgcacagct acaaagtgaa aaaggttgtt cacgggatgg
    14881 cccatatcac cggcggagga ctggtgggca atatccctcg cgtgctgccg gcagactgca
    14941 atgcggtgct gaagaaaaat gcctggccgg tgccgaagat attccgctat ctgcagaaaa
    15001 tcggacctgt agaagagtat gaaatgtacc gcgtttttaa tatgggcatc ggatttgtgt
    15061 tgattgtcgc agcggatttt gccgatgcga ttgcggccaa gctgaccaaa tccggcgaac
    15121 aggtatatcg cattggaatg attacagcag gttccagaaa agttgtcttg aaataacatc
    15181 ctgttttaag acaacggctt tttttaccct gcggtttccg gtctcaaacg gcatcaagga
    15241 tagaaatggg caaaacatcg gcaaatcggt cgagtcggtc tgctcttgca gcaggcaagc
    15301 attctttgcc ggcattaatt ctctacagca tattggcgat ttatctgttt tatccctata
    15361 taaaccgctt cagcacagtt caatttctat atgtaatcaa tcccatcgcc gcagcactag
    15421 gggcatatat cctcagccgg cgctggcttt accacccggc agcttcggtg acagccggtg
    15481 ccgcatatgg ctttggcccg ttcggattgg gatttaccgg ttttcatcca ttggcaggat
    15541 tctcctggat gatgctgcca tggcttttgc tgccggcggt ttactggcgg caggggcaaa
    15601 cgccggatgg gctgcggttt ggcgggcgtg ctgcattttc acttctgccg tttgtatgga
    15661 tggttcttct gttccgtatc gccgcccagc cgtggatagg gccgtttgca cttcttcctt
    15721 taactgcgtc gctgacgctt gaggacttta aggggcttat tttccctctg catgaggccg
    15781 gacagcatat tgttttcggt ctgtatccga cggcatttgt gatggcgctg atgggattgt
    15841 ttgtatatgc caagctgcag cggatcggcc cgctgattcc tgttgccgtt gggacagtgc
    15901 tggcattttg cgggccggtt tttcaggtaa gccccgttgt atgggcggca ttcccggtgc
    15961 tgtttttatc tcttctaagc ggattgggta ttgaagcgct gtcggcggct ggaaaagcag
    16021 attccaagtg ggttgccgtc tgtgcaatct ttgcgacggt attatccgcc tttttcgggg
    16081 gactttcgat tggcatcgtc cttatttcgc cggaggtatt taagctgacg gcattgctgt
    16141 acggcttggc agcagctgcc ctgtggcttt tattgtgcct gatacgtctg cagcttcact
    16201 ggccgctggc ccgatgggct cttctgacct gtgcagccgc aatcgacttg ttcttggcaa
    16261 gccgctattt taccgccaaa ctgttttaag ccgtatcggc aggcctgttg tcgcaagccg
    16321 ccggccaaaa ttaaatggtt tagtcctttt tcttttgcgg cacaggcagg ccgagttcgc
    16381 tgagaacctt aatcatatct tcccagacac gtttgcggtt ttcataggag taattgcgca
    16441 gcagataggc cggatgatag gtcggcatca gcttgaccgg cggagcctcg tcagaaactc
    16501 ggtattcaaa aaatcggccg cggagtttgc cgatggattc attggtattg agaagcgttc
    16561 gagccgccgg cgctccgagc gcaacaatta cctgcggccg gatcagcgca atctggcgtt
    16621 tcaaaaaagg ccagcaattg atgatttctt ctgcttttgg atctcggttg cccggcggtc
    16681 ggcatttgat aatattggcg ataaaaacat cctgccgctt aaggcccatg gcggtaatga
    16741 tgtcggtcag aagcttgccg gccctgccga caaacgccag cccctgctgg tcttcctcgg
    16801 ctccgggacc ttcgcccaca aacatcagcc gtgcctttgg attgccttgt ccggcaacag
    16861 gattgatgcg gctgcggcca atctcgcatt ttcgacaggc gctgatttcg gcagcaattt
    16921 tttccagcga atcatccgtc aggaattcct catccgctgt ttttgcacag accgcaccgg
    16981 acagaacatc agactgagcc cgctgcggcc ttttgacggt aaaatcgccg acaaaaaagg
    17041 cctccatttc caaatgctgg aggaaaattt tatcccaatt gacctgttgt tccatatccg
    17101 aagtatagga gattgccggc tattttcaaa caaaaaaact tggcacaaat ccgcatcctc
    17161 tgccgttgcc aagacctatt tcgatgtttt gggagcttca aaatagcaca ccaacttttg
    17221 actgaacaca tccgcttcga gccggagaga tttgtctgtt atttcataaa caaaaacagt
    17281 ctgactgaac ttttctgctt ccggcacaag gcgtgtcagc agattgggac gcttcaggag
    17341 caatcccggc tgaaataaca catcgctttt ctcacagaaa agagcgatat aaaaaatccg
    17401 ctcttccaca agatccataa agaagtggct gccgtaggac aattccggca tcagattgcc
    17461 gccgggataa gaaatctcgc acagcactgt tacctgattg atttcggaaa accgcaccgg
    17521 aatgccaagc gatggcgtcg aggttcccca gcgtcccggc cccatcagca ttgtaaaatc
    17581 cgattttctg ctgcagaatt ggcgattgag cttgcctatc aaccgcgcca gttcgtattt
    17641 ttcctgctgg gttaatgcga tataggccga tgggtgcaca taaataatct tattgacggt
    17701 ccgtgaaata ctcccgccca taaagctgcc ctgcgtccgg aacagaatcc gctccggatc
    17761 cagccgttct gtaagccgaa ttcggctgcc gacgcccgag gcctgaaggg gacggcactg
    17821 tagcaggtta atctgaggcg gcagattgcc tgctaaattg acagtaaact caatatcgac
    17881 tggataatcg tagaactgct cgagggtttt gagcaagcgg cgcataacag cagccaaatc
    17941 cgtatgttcc agaagcaaat cgaatgtcat aatatactgg cgggggccgt caatacccag
    18001 ctgcctcatc cggtcggcgg cttcctgatc ttcagccgcc agcaggtgga agtcccagtc
    18061 gggattcgat gccaaatact gctgaatcgg cagcgtttgc tcgcagttat cggcaatatt
    18121 gagaacatct atatagtgct gtgaaaactg gcgctgattg cccgcactga cgggcggacg
    18181 caaagacggt gcatccagtg ccaccagacg cgggtaatca tcgtccacac ggttcactgc
    18241 acgcgtaccc aaaccggcta ccagccggag cagccccgcc tgaggatcca agtccttatg
    18301 ccatacaaag gtattgcagg acactccaac tccagctgca tccggataaa aataggaacc
    18361 atggggagaa ccggagacgc gctgcaccag caaggccatc tgttcgtcca tttggtccaa
    18421 gccccgctgg agacgatatg tcagagcatc ttcgttgacc gtgcttgcat aaattgtgcg
    18481 aactgcttct tcaaacttgg cataccgttc atccggatcg ccttgattga ccagaaacag
    18541 actttcgtat ttgcctgcaa atgcattgcc gaagctgtct tccagcaaac tgctggaacg
    18601 gacaataatc ggcgactggc cgaaatactc gagcatttcc tgaaattgct cacggatatc
    18661 gctggaaaat ttgcctttga gcagtccgct ctgaagttcc ggtgccttag caaaatagcc
    18721 ttcgggagtc ttttgctcca ttcgaagttt ccagagtcca ttctgtacaa tgtaggtatg
    18781 gaaaatatcc gaaccaatat agaaggaatc gtggggttca agccggcttt gccagtcaaa
    18841 ggaggcatcc tgcgacagga tttttcgtgc cagcagcatc ccaaccgatt tgccgccgat
    18901 atagccggta ccgatgagcc gggctttaat atccagaagg tcacgaagcg taaaatgcga
    18961 ctttaagaga gccagcatcc tctcctcgcg ccccatcata atacggcaga tagtttcaac
    19021 tgcctgtttg tattcaggat tatctttgtc ggtatcagca tagcggctga caagtgtttc
    19081 agcattaaga aataatctat cccaataatc cagttttcgg cgggttcggc tgcgggcaat
    19141 gtccgaaatg taggaaaaaa gacgggcggc atctacacta tccgtaacag gtgtaaattg
    19201 tgttccatca tcgcggtgcg gcagaaacat tgtaggcgag tatcggttcc acactttcaa
    19261 cggatgaata tagcgagtcc cttcaagatg ataaacatcc aacagcaatt gcgtggtgcc
    19321 gcgtatgcgg gcaacagttt gaaaagaatg ataatcccgc agaagtgcaa aataggcaat
    19381 cgtgtctaat tcatagagat aagggcatgt aaccgtaaaa aagtttccaa ccatcaaatc
    19441 ggttgcccat gttgtcagca ggtctgacaa gctgtcaaat acataaaaag cccccctgcc
    19501 ctgctgagaa attatctgat gaacctgtgt cgtaaatgtc tcaaagccgg tgtcggcatc
    19561 cagttcgacg atcgtcaatc ccggctgcgg ctccaacagc ggcaaatgct gtgcgaaacg
    19621 aaagtaaacc agcttgcggt gttccgcgac agcgcgagcc gcaaagggag ctgcaagttc
    19681 tttgaatcgc tcaatagcat ctacctgcca gacaacatta tcgccgtact gaagccgggt
    19741 cagaagctca tccagatgat tcagtcctgt actggggtaa ggaaatcgct ccatagtgca
    19801 cttggccttt tttgtattct ttctctattt tatcaagcat ccgatagact gcaaaaaata
    19861 aaatcccccg ccctgaaggc aaggggattt taatcattga gcaactatct gtttaacgtt
    19921 ttggtcaaca ggtttagaca ataccctgtg ccatcattgc ctgcgcaacc ttgaggaaac
    19981 cggcaatgtt ggcgccggca acaagatttc ccttagccgc gtatgcttca gcagcgccga
    20041 tgcactggtc ataaatcgca ttcataatgc ttctgagccg gttatccact tcctcaaagg
    20101 tccaattgag ccgcatcgag ttttggcaca tctccaaagc gctggtagcc acaccgccgg
    20161 cattggcagc cttggccggg ccgaacgaaa caccgctgtt gaggaagact tcgatcgctt
    20221 ccggcgtcga aggcatattg gcgccttcac cgacggcgat gcatttgttc ttaacgagcg
    20281 tctcagctgc ctgcttgtcc agctcatttt gggttgcatt gggcatcgcg atatcgcagg
    20341 gaaccgtcca aatcccttga catccctcaa catatttggc cttgggatga gcattgacat
    20401 agtctttgat gcggccgcgt tcgacttcct tgattctctt tacagtggcc agattaatac
    20461 cggcttcatc cacaatgtaa ccattgctgt cagacattgc gatgactcga gcaccaagct
    20521 gctgggcctt ttggacagca taaatagaaa cattgccgct tccagagctg acaaccgtct
    20581 tgcctttcca gtcggtgccg cgatcctgca gcatccgggt catgaaataa accaatccgt
    20641 agccggtggc ctcagtacgc accaaggagc cgccataggt caagcccttg cctgtcagaa
    20701 cgcctgtaaa gacattggcc agctttttat attgaccaaa catatagccc acttcacggc
    20761 ctccaacacc aatatcgcca gccggaacat ctgtatcggc gccgatatga cggaacaatt
    20821 cattcataaa tgcctgacag aaacgcatga cttcgttgtc gcttttgcct ttgggatcaa
    20881 aatcactgcc gcctttgccg ccgccgatgg gtgtcgtagt caaggcattt ttgaaaatct
    20941 gctcaaatcc caaaaacttg atgatgccca gataaacgga gggatggaaa cggattccgc
    21001 ccttgtaggg cccaatagca ctgttgaact gcacccggaa accgcgattg acctgaacag
    21061 ttcccttatc atccacccaa gctacacgga acatcaactg acgttccggc tcgacaattc
    21121 gttcaaaaac cttggctttt gcgaattcag gatttttttt gattacaact tctacagact
    21181 cagctacttc acgaaccgct tgatgaaact caacttcccc gggatttcga gcagtaacct
    21241 gctccataat actgtcaaca aactgtgtcg ccattgtctt ttgcctttcc ttagaataac
    21301 gtttttggta ttcttaatcc gtacgacaat aaggattttt actttttatg ctaaaccgct
    21361 ttttcggtat ttaccgcgct tttgttcgtg cgcatcagca gttaaaaaaa tgaaaacaaa
    21421 gcctttacat tctatcatgt gtccttataa tagtattaac tattgcttta gaccaataat
    21481 aatttaagat actataaatc agtttatcta ataacacaat gaatctggaa atggctaaaa
    21541 tcttttgcga tttggcggag ttaaagaact tctcccgcac cgcggaactt cacggcataa
    21601 gccagtcagc agtttcccag caactagcgc atctagaaat ggtgcacaat acccaattta
    21661 ttaatcgcaa ggaacgtcct ttggaattga cggcctcggg acaggcattc taccgtgcct
    21721 gcaaagacat ccttgaacgc tataaccagt tcaaccatga gctcgccagc ttaactaaaa
    21781 ctacctgccg cgtcaacatc gcatctattt tcagcattgg aatgcacacg ctgcagccct
    21841 atataaaaaa atttatacga aactaccccg acgtaaacct tgttgtggaa tatctggatg
    21901 ccgcagaaat ctatacccga cttctcaagg gaagcattga tatgggagtt gtcgcccttg
    21961 ctagacatga ccgcgcgacg caggtttacc catttgaaaa tgagccgctg gttgtggttt
    22021 gtgcgctggg acatccgctg gccgaagcaa aagagattga catccatcag cttcagggca
    22081 gtaaatttat tgcattttct aaaaatgtgc ctacccgcca gcatatcgac agcatcctta
    22141 aacgctatgg cgtggcggtt cagattgcaa tggaatttga caatattgaa accattaaac
    22201 gagctattga aatcaactcc ggcatcagca tcttgcccaa gccctgcgta cagacagaag
    22261 ttaccagccg cacactggtt gaactgaacc tttctaatga gcatttcttc cgccctaccg
    22321 gcattatcgt acgcaaagac caccaattaa acaaggccgc ccaagctctt ctggatttaa
    22381 tgcgaaaaga cacatcaatc gaattgtaaa cagcaggaca ttatgataaa gttaaaatct
    22441 atgcagatac tgattatcgg attatgtaca gttttgaata tctgggcatc ggatgtatgc
    22501 ccaaatgctg ataaaacaga ttcatacaat tctcaaacag ctgattcagg ggcaggagat
    22561 ctcgagcaaa ttctatccag aatcgttgag tccacccgac aattaaaatc ctgtcaagcc
    22621 cggctgtcgt atcttttcgt tcaggatcca gatttgcttg cttcccgcac gctgcggact
    22681 ggaacactgt attacatcaa tggaggagaa aagtcccttt taaggataca ttttcaagat
    22741 ataaaacagg atgattttga gcccgaagca agacgggaag aatacctgtt tgacggtgtt
    22801 tggctgactc gtattgattt taagcttaag caggtggatc aattccagca ggcgccggaa
    22861 gataagccga tggatgtgtt tgagctgata agccataatt tcccaatagt aggcttttca
    22921 ggtacggata agctgcagca ggatttcaat atcgctctaa aagaaaaaaa taacgaccca
    22981 aacgagccga tttgccttct tttggaggta aaacaaggtt caaagtttca cgaggaatac
    23041 gaaaaaattg atttctggat agatcctgcc aactatttgc ctaaacgtct gctggcttac
    23101 gccgtacagg gggatttata tgatatctcg ttttctgata tagaaatcaa caaaaagttg
    23161 gaaaagtcgg tttttacgat tgaaacgccg gccgatttcc gtaaaaatgt agagccattg
    23221 aagaattctg gtgataggaa aggaacatag agtatggcca aaggactggt gttgtactat
    23281 tcccgaagcg gcaataccaa aatgatggcc aacgcaattg ccgaggccat ggaaaaagcc
    23341 ggattgccaa ccaaatgcaa aagcgttaca gatgctaaag tcagtgattt agtcgatgcc
    23401 gatgctgttg ttgtagggtc tccgacctat tatggacggg cggcggcggc tgtggcacag
    23461 cttttcgacg aatcagtcag caagcacggc aaactggacg gcaaggttgg ggctgccttc
    23521 agcagctcgg ccaacatcgg cggcggcaac gaaactacaa tctgtgaaat catcaatatg
    23581 atgctcattc acgggatgat tgtgcagggc gatccgcagg gcgaccatta tggtcctgtt
    23641 tccattggaa agccggacga gcgtgtattg acacagtgtg ctcgccgcgg tcaacgtgtg
    23701 gctgcattga ccctaaaatt attcggataa atcaacaatt tatatttgaa aagcatccaa
    23761 agatatgtaa ttatttcgtt tacttgaata agagcaagac taacagcgat tccaaggaga
    23821 tacgatacat gcaggcttac gaagaactga aacagctggt agcagaaatt gaagccgata
    23881 ttaacaaagc agagggcggc aataaggcag ctggaacccg tgtgcgtaaa cagatgcagc
    23941 gaatcaagca ggccgcacag aatgtccgaa acaacattct cgagatccgg tccagcgaat
    24001 agaccgcttt gcccattttg gaacctacga ttagttttgc cggcatcgcc ggaagaagag
    24061 gtctgagcat cgtaaacggt taatacccta taaccattcc gtaagaggag agtatgcctc
    24121 cagcgctatt ggttgattta tcaacaattg atttgaatac tgtggaatat gaccgcagcg
    24181 ccatagctga ggttaatccg cagtcgtacg agatgtctca actggacggc atcatctggc
    24241 acgatttgga gcggatgctg tgcgtgggct ttaaagatgt taccgaacat gagttttggg
    24301 ttcgaggtca tataccggga cgtcccatca tgccgggcgt tatcatggtg gaagcggctg
    24361 ctcagttatg cagttttttt atgaaacgca tctacggttt gaccggcttt atcggtttcg
    24421 caggaattga taataccaag tttcgtgaaa cggttccgcc gggcaaacgg ctttaccttt
    24481 tagggcacat tcacaaagtt cgaagccggc agttttccgc tatggtacag ggagttgttg
    24541 acggcaaaat ggtgttcgat accgtcatca gcggcatgcg ggtttgaagt gctgacgcat
    24601 catgtcaaga aaaacggccg acctgcaacg cagattcggc cgttttcata tatagcccca
    24661 aggggattcg aacccctgtc gccgggttga aaacccggtg tcctaggcct ctagacgatg
    24721 gggcctgaaa aactttattc catacggatc gcttcgactg ggcattcatc cacacaaaca
    24781 ccgcagtcaa cgcatttatc cgcatcaacg catgctttgc catcctgcat ctcaatggct
    24841 tcactggggc aggcatcaac acacgacccg cagcctgtgc aggttcccgg atcaactttc
    24901 gcaggcattt ttatcccctt tccttgaata gttaatatct tttcattctt aaaacagagg
    24961 cggaagttta acacaaaaca aaggaaaaac tcaatacttt ttttaaaaaa tttttaaaaa
    25021 ccaacacgca aaaaggacca acacaactcc aaaacaaatt gtagagttat cgtgtcgccc
    25081 tttaaaaatc aaacttcttt cagagggagt ttggatcggt ctgggtttga ggccctgtat
    25141 tggctcccaa agcagccgcc aagcggcggt tgagaggata ggtatcatat tgggcagccc
    25201 aatcaataac cttctggaaa gtcccgggct gggcactggc aagcagatac atcgccataa
    25261 accgcacagg ccaaaggtcg tgatgaaggt ttgctgagat ttcacgtatc agaccatatt
    25321 ccattgggac agatccatcc aaaagagagg ccatcgtctg gatgcgaact ttccagtcat
    25381 catctgcaag acttcttcga accgcatcta ccagaagcat ccgttcggtt tggatatgcc
    25441 ggaagtcggc ttgactcagc tcaaaggcct tttgctccgc cagcaaaccg gcaaacagcg
    25501 aagcggcttg aagtttctgc cccggctgcc ctttggacag agcctccaga cgctgaatga
    25561 gaaagtcgcg tgacagcaca acaccgggcc gctgaatggc tgcccgcaca ggattgagac
    25621 cttggagccg attttccact ctgccgtcag gcgaaagaac cggatccaga taaacagtaa
    25681 aatgaacttc gacatctgcc tgcggataag cagccagcag tttgcggagc ttgccggtgt
    25741 tcagatccag cggcacagat acatacgatt tcggctgaat cggtctggat ggtcggactt
    25801 ttgtcgtaag cagattggga atttccacat tcaaatcccc tttaaggaca gcgtctattc
    25861 gaattacgcc ggaaagacaa ctgccatctg ctatgatcaa tggttcggtg ccggtatttt
    25921 caataatcag ccgaggtgat atctccatcc cgtaatggaa atcagaacca ttgaaaagca
    25981 atcgcagcgc ataatggtcg gccggcacat taaaagccgg gacaacccgc ttgccatact
    26041 gctgctgcag gtctttttga atcgtctcaa gactgaccga cgggatataa tccgatccct
    26101 gatccttgat aaggcggatg gccttgtctg ctgcaaacgt attgggggcc atttccacag
    26161 cagtcctaag agagtccaga gcggcctgtt tctggtcagc agcaagttca accaatgcca
    26221 ttgtcaaagc tgcaatttgg tccgtttgcc ggaggggctg agcatactgg gcagccaaat
    26281 cgtattgtcc atgaacagcc aatgcataag cgaacattac gcttaccccc tgccgctgcg
    26341 gagcttcttc aaaggcccga ttgctccaag c
//
LOCUS       DAOTBO010000028        26105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1544_length_26105_cov_5.847255, whole genome shotgun sequence.
ACCESSION   DAOTBO010000028 DAOTBO010000000
VERSION     DAOTBO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 26105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26105
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_26105_cov_5.847255"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLE88_05170"
     CDS             <1..368
                     /locus_tag="PLE88_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75892.1"
                     /translation="TILATGQGQLQAATVPAKLQALNLIESVRPAGSANPAAALRYAM
                     QLKDASGSGPELIYFLTDGFDFNLPQSAHFADEFTQMHQTLAPAAVVHAIGFWPQPQD
                     RKTLQQLANSTGGEYIEVK"
     gene            446..1252
                     /locus_tag="PLE88_05175"
     CDS             446..1252
                     /locus_tag="PLE88_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOM75893.1"
                     /translation="MFWNAEQVVLGQAEPTQSAATLYHQFFTAGGPIVWFILLPMSIF
                     TLYLAIDLLIVIRRSRLLPPAISSDIATYAVRYGIGALSGRLSGQPDLLSRSLLRALE
                     RSRQFGGQVESIHQFAAEALQEQGISLLRKAQWCQIIGSVAPMVGLFGTVYGMIRAFN
                     LLGQGGEGPRYELLAEAISIALVTTFWGLLVAIPALFVYGVFQTRIEAFVSEAATELE
                     ALLGRIFETSGIFQNELGRQESSAAQTKKMPDKNPSRPSSQQALTAEVQK"
     gene            1249..1707
                     /locus_tag="PLE88_05180"
     CDS             1249..1707
                     /locus_tag="PLE88_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOM75894.1"
                     /translation="MKRLLLHNRPFETELFDMTPVIDVVFLLIIFFMLVCQFIAAEQL
                     KVSLPDQIKTAQPQETAANLPLTITVLPASEGRILCAVGSQKLADVQGEDLARLICVA
                     VDESLAAHAGGKTVHLRCDKSIQFGQIKYILSGIAQSRADNLDWAVLTEP"
     gene            1729..2970
                     /locus_tag="PLE88_05185"
     CDS             1729..2970
                     /locus_tag="PLE88_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOM75895.1"
                     /translation="MYELVTICISLGLLAFLLHLKMRLGRAMLLTAFALAVLLGTAPH
                     MLLQTAAQEWKSKPLGQTTGYLFVSLTTLLTFVNILGIALTHTGISQRLLPSIQGLFR
                     SRRAALASIPLMMGMLPTPGGIMLSAPMVRQLGDQIGIERSRQAALNYLFRHQWESVW
                     PLFPAVPLIQGMLGISAADLVLHNLPIMLCGTAAGIIFLLLPGLPPKQIDIRQERFTD
                     NLAGFLHAFWPIGITALLYAAAGITPAAGIIAVTVLFLILHKVRFKQWVAIFKSGIEF
                     DVVLLILGALLFKLIIESSRSVDAVVSFLTAMHVPPLFLVFFLPFLVVFLTGLTMPTA
                     AITFPFLVPFIGTGPAAKLGIETLAFSGIICGLAMTPIHLCLSLSASYFDTSLLRIIG
                     RMAGPIVSIAAAGAVMALMAG"
     gene            complement(3047..3649)
                     /locus_tag="PLE88_05190"
     CDS             complement(3047..3649)
                     /locus_tag="PLE88_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOM75896.1"
                     /translation="MKQQVAAVSIIALICAGIGVGAGVLIMKPKLSQSAKTIEELQLK
                     MQQTKTESEDALQRASGEIVRLKTELSRANSTITQARNDLARATAELARLQAEAQRPA
                     KEADKTSQEEAQTSAVAASQPSTSASPVSAAGSGAGLTPAAPKTAAVEYVVKEGDSLW
                     KIAASQLGSGVRYEEILALNPQLKKDSTLVVGSKIKIPAK"
     gene            complement(3745..4377)
                     /locus_tag="PLE88_05195"
     CDS             complement(3745..4377)
                     /locus_tag="PLE88_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75897.1"
                     /translation="MMTGRKKQYRGFALLVVLIAAAILMLLYFVQIDVFVAPEPKSSQ
                     PAAFEQRPWLLEDLLAGPGQSIPMPSPPKAQIDRPFERTAAVSRNQSSRGRITAAFET
                     DGRIVAQWNGTWTEGPTNFQINAVMKGNISVSKTYEDAAGKDKSRLFFIARGQYCKTE
                     EGSVGSVQEEGTAYLLGWLCSDYSAVGCITITTDRKWTAEYRFEWPAEDI"
     gene            complement(4390..5418)
                     /locus_tag="PLE88_05200"
     CDS             complement(4390..5418)
                     /locus_tag="PLE88_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOM75898.1"
                     /translation="MQNRNQLFDFAIAQHATDIHICAGAPVQFRIGRKLKPVTEEILT
                     PDKSRQICYEMLTEEQIRKFEQQLDFDLMLAYHGSRFRINVGFFNGTVGATIRVLDGT
                     PRPPDELFLPPVVKSLAQKEKGLILITGSTSQGKTTTLASMIDFINEHQKRHIVTIED
                     PIEYLHANKKGIVRQREVGRDTASFSSGLRAALRQDPDVIAIGEMRDYETIKIALTAA
                     ETGILVISTLHIISIDKMIERLLSYAPEDSENTIRFLLAETLQGIIHQELIPLIGGGQ
                     RVACEIMINTAGVKNIIRHRGGYHLRTVIDTGKRYGMCTMAESVGALLEANLISAESA
                     KRVLSNYE"
     gene            complement(5642..7135)
                     /gene="tig"
                     /locus_tag="PLE88_05205"
     CDS             complement(5642..7135)
                     /gene="tig"
                     /locus_tag="PLE88_05205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOM75899.1"
                     /translation="MAQENTQTTEETKEKIPNVVTVSDAGPCKKKVAIEIPAEVVHEK
                     LDEKYKELRKEAILPGFRKGRAPIRLLEKRFGTDISRQVKLELMAEASEAALKDNKID
                     ALRDPDIDHEKVELPEEGPMKFEFEVEVRPEFDLPELEGIEIEKPKIDITDARVEEEL
                     MSLRKQAGVWIPKDGPAAEGDRVVADVILVTEGSPEHDKYDNIEISVRRTGFVGAVPV
                     DGLDALLVGAVSGTEKKTTVEVPATFYNEQYRGKKVDLTITVKEVKKLEPAEMNSEFF
                     SRFSVSDENELKENIREQLSARAERQARAAMSEQVYAYLRENTDFELPEAVVAEQSLS
                     ILQRQYTNMLMQGMTRQQIDEQMEQLKAGSEEHAADQLKLYFILDKIADKYGITATEE
                     EINGHIAYVAALRGRRPEKMREELIRDGSLAQFALQVREEKCIAKILETAKIREVEPE
                     KLKKPAPKKSARKPARKKETGHEETEVVQERKKTESKRSKKADKTDE"
     gene            complement(7353..7424)
                     /locus_tag="PLE88_05210"
     tRNA            complement(7353..7424)
                     /locus_tag="PLE88_05210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7390..7392),aa:Gly,seq:gcc)
     gene            7895..9181
                     /locus_tag="PLE88_05215"
     CDS             7895..9181
                     /locus_tag="PLE88_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOM75900.1"
                     /translation="MVKPFFDLQAHQTTVKTEVIAGFTTFLTMAYIIFVNPNILAEAG
                     MDKSALIAVTCLVTAVSTIATGLLANAPIAMAPGMGLNAFFAYTLVLTRQVTWQTALA
                     VVFLSGLFFLILTLAGLRKKLVEAIPQSLTAAISVGIGLFITFIGLQNLGLIVAHPVT
                     LVTSAHLTPTILVGLAGLAVMIIFEMRRIPGALLIGIAFSTALAALLGYISLPQKLVS
                     ININILPTAFKMDFVGALKWGMFSSIFTLMFIDMFDSIGTLLAVAPQAKLVDAGGKFE
                     KLDRLLTIDAAATMFGAVCGTSTTTSYIESAAGIAQGGRTGLTAVVTGILFLAAMLFV
                     PLAGIVPNYATAPALIMVGLFMTRQIRLIDFSDMQQGLPAFIIMVMIALSYSISTGLA
                     FGFITYTLIEILTGKFRNIKPAMWVIAVLSVLYFCV"
     gene            9305..9769
                     /locus_tag="PLE88_05220"
     CDS             9305..9769
                     /locus_tag="PLE88_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOM75901.1"
                     /translation="MMLKVTSSAFEHNGMIPAQYTADGEDISPPVSWEGLPEDTQSIA
                     LICDDPDAPMGTWVHWLVWNIPPQMTGLAERFPTDAQLDSGIRQGITDFGQSGYGGPA
                     PPSGTHRYFFKVYALDTMLNLPAQADKRQLEKAMSGHILAKGELVGKYSRRR"
     gene            complement(9782..10915)
                     /locus_tag="PLE88_05225"
     CDS             complement(9782..10915)
                     /locus_tag="PLE88_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM75902.1"
                     /translation="MTVIVRKPDIIEKLKILSADAQYDLACACGSTKDEHRRRSSEGK
                     WIYPITLASGGRASLFKVLMSNVCSNDCKYCPLRENQDIRRCSLSPDEIVRLFLDYYN
                     QGKVFGLFLSSGVIGTADSTMERIDAIARLLRNRHAFRGYIHLKIIPGASIPAINEAV
                     SLASAVSLNIETPGAEYLSKVSSRKDYIRDIIEPIKHISRLTARGSRWHRVKQTTQFI
                     VGAAGEPDRQIVRYMGGLYERLKLDRIYFSAYQQGLGSPDLPAEQAASREDILTREHR
                     LYQVDFLMRKYGFKDSEILFDAHGQLSLTEDPKTTWAKMHPDFYPVDINHADKFELLR
                     VPGLGPAAVNRILQQRRSGRICCIEDIGLAGKLLAKAKPYLAF"
     gene            11140..13116
                     /locus_tag="PLE88_05230"
     CDS             11140..13116
                     /locus_tag="PLE88_05230"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOM75903.1"
                     /translation="MPNTLTYKILQKHLVEGELKAGQPIGIRIDQTLTQDATGTTAFL
                     LFESMGIARVQTELSVSYVDHNMAQFGPENHNDHLYLQTVAAKSGVYHSRPGNGICHQ
                     VHLERFARPGATLLGSDSHTPTCGGMGMLAIGAGGLDVAAAMGGGPFYLTCPKVIGVE
                     LTGRLPDWVSAKDVILKLLNILTTKGNVGCVVEYFGRGVQTLSVPQRATITNMGAELG
                     VTTSIFPSDERTLEFLELQERDDVYVPLSADPEAPYDRVIQIDLSALEPMAACPSSPD
                     NIAPVSEIAGRKVHQVIIGSCTNSSYMDVMMVAKTLQGRKTDPMVEFAIAPGSRQVLE
                     MIADNGALTTLIAAGARILESACGPCIGQGFSPADGTVTLRTFNRNFAGRTGTPGDQC
                     YLVSPETAVASAIMGEITDPRNLKKMMGIAYPRIKMPKAFLIDDSMIEKPVSLDEISR
                     VEVVRGPTIVVPESPRPLPEHLTGQVLLKCGDKITTDHIMPAGAFLKYRSNVPQYSKY
                     VFNCFNEEGKPTFAERAMALKKQGIGGIVAAGESYGQGSSREHAALCPMYLGVRVILA
                     KSIERIHKANLINFCIVPVEFSNPDDYEAIHPDDIVEIPDLLAAIKLRDTLVVLNKTA
                     GIEIKGKLTLSHRDRDILLAGGLLSYTHKKAVAR"
     gene            13113..13664
                     /locus_tag="PLE88_05235"
     CDS             13113..13664
                     /locus_tag="PLE88_05235"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOM75904.1"
                     /translation="MSTSTIITVFGTSKTQPGEPEFETAKILGRLLAKKGFTIANGGY
                     SGTMLATAQGAAEADGQVIGVTCRAFKRGKANPYISREIQTNSLTERLAILVELGKAY
                     IVLPGGTGTLLELADVWEHKNKGFSNAEKPIILMGAFWKPLLEMMGRADAESLRSVDY
                     AEGPEEAAAIITAKLSTKQVDYA"
     gene            13657..14478
                     /locus_tag="PLE88_05240"
     CDS             13657..14478
                     /locus_tag="PLE88_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75905.1"
                     /translation="MPKKLYCLFFIIIFSFASLAADQDAKSAVKEAVGVSVVDDGFLA
                     DGVEGWAKKETSSGVWSFVPEKMIDAGGGKVIASGQPVQMLPCAVLEQIVKLAGQNKQ
                     LQIRLWAVFTKYKYANYLYSVYFLPIQDQSPKPPAESQTPAGTQHKAAQPEDSIIPEE
                     ILAQIKSTRTPDLKKFQQIAQVTGDVNLIGRTGYLIEQNGKRYFQPDGFGMKIDSQPL
                     LLLPNEARQTTERQLNLTPGRQRYSVSGLITTFEGQRYLLLRRAIRTYTHGNFTP"
     gene            14459..15793
                     /gene="hisD"
                     /locus_tag="PLE88_05245"
     CDS             14459..15793
                     /gene="hisD"
                     /locus_tag="PLE88_05245"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOM75906.1"
                     /translation="METLPPELRSMNFDSILLRTTDADYAQRLERLKAEHSMGGFLQS
                     RGQLIQTVRNIVADVANRGDAAVAEYTEKFDKVKLQPAEFRIPAEELKKAYEQLDRPL
                     LKSLKKAIANVRKYQSDIFIGRKNRYPGIRYMPLKRAALCIPGASAPLPSTVIMTAVP
                     AQAAGVQELAVFSPPRYNGSIHPVILGVCCELGIAEVYRIGGAQAVAAAAYGTQTIRK
                     VDKIVGPGHDVVQLAKKEVFGLVDIDSFAGPSDVLIVANDKADPAWVAADMLSQAEHN
                     PGAGLVVTDSLDFARKVLAELGRQCAALDRAEAAARCLMDYSAVVVCESMEAVIDWAN
                     DFAAEHLQVQCGRQSKAISKKLINAGAIFIGPYSPVAVGDYWAGPSHTLPTRQTSKFF
                     SALTSNDFVKSTSIIEYNRQQLEDAADDIVRLARIEGLDAHAKSIQKRLKRP"
     gene            complement(15834..17561)
                     /locus_tag="PLE88_05250"
     CDS             complement(15834..17561)
                     /locus_tag="PLE88_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75907.1"
                     /translation="MNQQDLEKYSSAITLSDMEVFVFPELMYALVLANIMSPVIWRWR
                     EDPAFKKLEGKNIYRRLMRMRQYVMDEYEFNLDLQTWGLTRKDVELSRFKDFLSPDDI
                     ARSNALFGYTGDSYYFDVDIRRHFGLDQYDGDVIPYWKTETVEAMNAFRRKEGYAVGA
                     GECVSLSALYAAAAFIVCGLSLEDIYMVLTPLHSQNFFDVSDGIITNNRRLVTKSMWF
                     NGTEISDKAQRALRNEQITIVAHHTGYIHCFYQQATIDPQRYQHFCRRLSDYLQTELN
                     HLNLANFLRSRGKYQKYFQFCRHRRGERMFVKAENLFGYEHGSKFRIYDETFEKLLEE
                     VSEDDFMVYPYPGRICCEQLMAFIQYEKIDIQSSADRLKLARFIAPYTGQDAEACVSE
                     MADFLKIEPKLPAVDKTYQKTEPIKLSPEWNRQQIAEYLDSMRSRNITADLAFYAYRD
                     MTRCDWRPFMKAALERSPVSIEKYRDSDIPDVYQQLCSLPDESIYEGPRLAQPDEVAN
                     YNTGDGLEKAITLANIIHQRQPELTLNLTVDGHTVVLQADREYAFQSAKGLKKHIHME
                     QAGYQVSDG"
     gene            complement(17575..18570)
                     /locus_tag="PLE88_05255"
     CDS             complement(17575..18570)
                     /locus_tag="PLE88_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOM75908.1"
                     /translation="MPANLTPRYKKAEEQYKAAATDQERLLALEEMLREIPKHKGTEH
                     MQADIKRRMSKLKEALESESKRSGSRHGDIFHIPRSGAGQAALIGMPNAGKSSILAAL
                     THAPVTVAPYPFATDKPVPGMAMFEDVPIQLVDTPPITADYAPSGLVNTLRGADLLAI
                     VIDLSADVLEQMDVCLRYLDSHELILDEAAANPDSREYRLGRKTFVLAAKADIAPPGT
                     LETLKELTQRPFEYIAVSAELRQGLDTLMRRLFEMLDVVRIYAKKPGAEPDFKEPFTL
                     KRGATVYDLACHIHRQLAEKLKTARAWNAPGIHDGQNVPREHVLSDKEIIELHFG"
     gene            complement(18692..19447)
                     /locus_tag="PLE88_05260"
     CDS             complement(18692..19447)
                     /locus_tag="PLE88_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOM75909.1"
                     /translation="MIGYDLHIHTEYCGHAPEMTVEVICRRADELQLSAIAITDHIYE
                     PADMFGIEQIRKEVRTCQPRCRVYIGAEIDVDADYTDGRLVTDVFEGLDYVIAGFHYV
                     PTQGHYPRSPDDRRLDEKTFLQVWESSLLGIVSNPAVHTLAHPGRMLAACMDLDIHFE
                     YGLQVMRKAAEISARNHIAWELNELTGSRLPAYWQSQWHRIYQIALDAGVKLVYGSDA
                     HTPAAIGTHAFVNQLLRMLPPNCLSQPEEIILR"
     gene            complement(19444..20601)
                     /locus_tag="PLE88_05265"
     CDS             complement(19444..20601)
                     /locus_tag="PLE88_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75910.1"
                     /translation="MHTDQQHKQWMQLMVLTAAHFVLDAFPGLMHTVLPGLQQSFHLS
                     VAAGGILLTAFLVSANGIQVLIGHLRPDRQQPFFLYGGLILVCSILLFTAVPKNSIVL
                     VWLSLIAVLSGLGVGMTHPEALRAVHRLNGISSALGSAVFMAGRVIGFAAGGWGSTHL
                     FKMWGLFSIIPFCAASLGVLVLTLCFRVRLAVESDEASRRAAQSSNLYVSFWLVMAIA
                     TLSACSVQVLMWIVPQRLNEIGTDLTVGGVAVSLFSMAGGIGGIIVARWADRYGEIKL
                     IEWMLAVGTPFVGAYLLLADRSWAAGLLCVGGFFCYGAYPLMISAARNSRGPNLGRRM
                     GFIVGGIWLAACLLPMLLGPAAHRFGTGPILFCVPVGFLAAWILAFVSGKR"
     gene            complement(20604..21389)
                     /locus_tag="PLE88_05270"
     CDS             complement(20604..21389)
                     /locus_tag="PLE88_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM75911.1"
                     /translation="MTPAVVELDKVTFCRKKRMILSDVSWRIEAGKHWALLGANGSGK
                     TTLLKILTGYEWPTFGTVRVLGERFGICDIGRLRKLIGWVSSAMTQRLPMQDLAVEVV
                     ASGLDASIGLYRQISPEEFAKSLDMLKKLRADSIAHQDYGTLSQGEMQKVLIARALVC
                     EPKLLILDEPCIGLDPAARQRFMNDLAVLARQDNAPTIILVTHHIEEIDPWIQQVLLL
                     KNGQVLAVGEPDDVITSRRMSELFDYPCSVFRQGAEFLLRIDN"
     gene            complement(21390..22289)
                     /locus_tag="PLE88_05275"
     CDS             complement(21390..22289)
                     /locus_tag="PLE88_05275"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOM75912.1"
                     /translation="MANVVLQTDIKGVQCRRGKVRDIYDLGDALLIVASDRISAFDVV
                     MKCGIPYKGQVLTQISKFWFDFLTGVVENHLISDNVENFPAPFSNYPQQLAGRSMLVK
                     KTKVLPVECIVRGYITGSGWKEYQQNGTVCGIKLPKGLKQCEKLPEAIFTPSTKAEIG
                     THDENIDFDACVKLVGRETAEYIRDKSIEIFTKAGQYAASKGILLADTKFEWGLIDGR
                     IILIDEVLTPDSSRFWPADKYQPGREQESFDKQFVRNYLEEIRFNKSGPGVVLPDDII
                     SKTSAKYIEAYERLTGKKFIPAE"
     gene            22561..23085
                     /locus_tag="PLE88_05280"
     CDS             22561..23085
                     /locus_tag="PLE88_05280"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOM75913.1"
                     /translation="MKHKSNIVLIGMPGSGKSTIGVLLAKRLGMSFLDTDVLIQAHWG
                     RSLQELIQLHRMEGFCRIEQEYVRSINAVNTVIATGGSVVYYKDAMQALGVNGLIVYL
                     RLPLELLQKRLGDLNLRGVVLEPGQTLAALYAKRIPLYEQWADISVDLNGLNHEDSVK
                     AILDKLNAAKRKEH"
     gene            23088..24332
                     /locus_tag="PLE88_05285"
     CDS             23088..24332
                     /locus_tag="PLE88_05285"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOM75914.1"
                     /translation="MKLEKYSMGIGDRFGHQGKAQLSALITAKSEGIHVIPVWNKSNR
                     EHSIIGTVPADTRAEAEAATAAMGWTDAYYVDADHINLNNVTPFIACSDFFTLDVAEA
                     IGKPCDKAALDKFVRRHSNLTGQLSFDGIEQTFDITEDIIRRIGSKYLFAVQEAGRIY
                     RHLVSAKGQGNFITEISMDETDTPQTPLEMLFILAAAADEGIPVQTIAPKFTGRFNKG
                     VDYVGDIEQFEKEFFANVAVIAYAIRQFGLPDNLKLSVHSGSDKFSIYEPIRRALKKF
                     NCGLHLKTAGTTWLEELIGLATAGGDGLKIAKEVYAKAYAKMDDLCAPYATVIDIDKH
                     MLPSPQEVYGWDGLTYAAALKHDPKCELYNPHLRQLLHVGYKIAAQMGNRYLDALAEY
                     EDIVAACVKENILTRHIRRIFI"
     gene            24748..25266
                     /locus_tag="PLE88_05290"
     CDS             24748..25266
                     /locus_tag="PLE88_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOM75915.1"
                     /translation="MVFHEMVPWKKDRRNVKVRTEQDEFPLMSLQRRMNRLFDDFFGD
                     FGDFSLSPFRSLARGANEFIPRIDVTENDTEITVTAELAGMDEKDIEITLQDDVLTIK
                     GEKKAEREEKDVNRYLCERSYGSFCRAIELPAEIQQDKIEATCKKGILTVRLPKTPAA
                     QSKAKKIEVKGE"
     gene            25507..>26105
                     /locus_tag="PLE88_05295"
     CDS             25507..>26105
                     /locus_tag="PLE88_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75916.1"
                     /translation="MADNNTIPNGISSLPVKSFMTVGPTLHYSHSHVHAMWGITVFVF
                     VIVCYFWNQIQIGGPVSIDPMELADTSLFRLGQIVVRPISIYEYPWHIIVLGTLMGIL
                     AVAPVLVSQLYSFRYSLPLVLSIFFIGQLYLFSGFVLVSCLAAACRPLRFRSRIVAIA
                     LCMAPQLIYWAVWGGYPTADPVRWGFSFAPWIYAWLAALA"
BASE COUNT         6417 a         6721 c         6365 g         6602 t
ORIGIN      
        1 agacaattct ggctaccggc caggggcagc tccaagccgc aacagtacct gccaaattgc
       61 aggcgctgaa cctaattgaa tcggttcggc cggccggttc ggccaatcca gccgcagcac
      121 tgcgttacgc catgcagcta aaagatgcat ccggcagcgg tcccgaactt atttatttct
      181 taaccgacgg atttgacttt aacctgccgc agtctgccca ttttgccgac gagtttacgc
      241 agatgcatca aacgcttgca cccgctgcgg tcgtgcatgc aatcggcttc tggccgcagc
      301 cgcaggaccg caaaacgctt cagcagcttg ctaacagcac cggcggggaa tatattgaag
      361 ttaagtaaac aatcgtcatc aaaaaacaac cagtaaattt ctgataaagt gcggccatat
      421 ccgcttgaaa tattaggaca acaggatgtt ttggaatgca gaacaggtcg ttttagggca
      481 agctgaacct acccaatcgg cagcaacgct gtatcatcaa ttttttactg ccggcggtcc
      541 gattgtatgg tttatcctgc tgccgatgtc aatttttacc ctgtatttag ctattgatct
      601 gctgattgtt attcgccgca gccgtcttct gccgccagcc attagcagcg acattgcaac
      661 gtacgcagtc cggtacggca ttggcgcttt gtcaggccgt ttatcggggc agccagacct
      721 gctgagccgt tcgctgctgc gggcattgga gcgcagccgg cagttcggcg ggcaagttga
      781 gtccattcat cagtttgccg ctgaagccct tcaagagcag ggcataagtc ttttacggaa
      841 agcccagtgg tgtcagatta tcggctctgt ggctccaatg gtcggcctgt tcggcactgt
      901 ttacgggatg atacgggcgt tcaatctgct cggacagggc ggggaaggtc cccgctatga
      961 gctgctggcc gaggctattt cgattgccct tgttacaacc ttctggggtc tgctggtagc
     1021 aattccggcg ctgtttgtgt acggcgtttt tcaaacacgc atcgaggcgt ttgtaagtga
     1081 ggcagcaacg gaactggaag ctctcctggg acgcatcttc gaaaccagcg gcatcttcca
     1141 aaacgaactc ggccgacagg aatcctcggc tgctcagacc aaaaaaatgc cggataaaaa
     1201 tcccagccgg ccgagctccc agcaagcgct gaccgctgag gtgcagaaat gaaacgcctt
     1261 ttgctgcaca atcgaccttt tgaaactgaa ctgttcgaca tgacacccgt tattgatgta
     1321 gtctttctgc tgattatctt ctttatgctg gtctgtcaat ttattgctgc tgaacagctc
     1381 aaggtcagtc tgccggacca aattaaaacc gcccagccgc aggagactgc cgccaatctt
     1441 cctctaacca ttacggttct gcctgcatcg gaaggccgga ttctctgcgc tgtcggaagc
     1501 cagaagcttg ccgatgtgca aggtgaggat cttgcccgtc tgatttgtgt agctgtggat
     1561 gaatcgcttg cagctcatgc aggaggcaag accgttcatc tccgctgcga caaatccatt
     1621 cagttcgggc aaatcaagta tattctgtct ggcattgccc aaagccgcgc tgacaacctt
     1681 gactgggctg tactgaccga accctaacaa acccctcctt gacccgttat gtatgagctt
     1741 gtcacaatct gcatatctct gggcctgttg gcttttctgc tgcatctcaa gatgcggctg
     1801 ggacgggcca tgctgctgac tgcatttgct ttggctgtgc tgctgggtac agcaccgcat
     1861 atgcttttgc aaacagctgc ccaagaatgg aaaagcaaac cgctcggtca gacaacaggg
     1921 tatctgtttg tatcactgac taccctgctg acatttgtaa atatattggg gattgccctg
     1981 acgcacacag gcatatccca gcggcttctg ccgtcgatac aggggctgtt ccgcagccgg
     2041 cgggcagctc tggcttctat tccgctgatg atgggaatgc tgcccacgcc cggggggatt
     2101 atgctgtcgg cgccaatggt gcggcaatta ggcgatcaaa tcggcatcga gcgcagccgt
     2161 caggcagcat taaactatct gtttcgtcac caatgggagt ccgtctggcc gctgtttccg
     2221 gccgtccccc ttatacaggg aatgctcggc ataagcgcag cagatctggt gctgcacaat
     2281 ctgccgatta tgctgtgcgg tacagcggca ggcattattt ttcttctgct gccggggctg
     2341 ccgcccaagc agattgatat cagacaggaa cggtttacag acaatttggc cggctttctg
     2401 catgcctttt ggccgatcgg cataacagcc ctgctgtatg cagcagcagg aattactcca
     2461 gcagcaggca tcatcgccgt aacggttctt ttcctgattc ttcacaaggt ccggttcaaa
     2521 cagtgggttg ccatcttcaa atcaggaatt gaatttgatg tggttctgct tattctcggg
     2581 gcgcttttgt ttaagcttat cattgagtcc agccgctcgg tggacgctgt ggtttctttt
     2641 ctgacggcaa tgcatgtgcc gccgttgttt ctggtgtttt ttctgccgtt tttggtggta
     2701 tttttgaccg gcttaacaat gcccacagcg gctattactt tcccattttt ggtgcccttt
     2761 atcggcaccg gtcccgcagc caagctgggc attgaaacac ttgccttttc ggggatcatc
     2821 tgcggacttg caatgactcc cattcattta tgtctgtcgc tttcggcaag ctatttcgac
     2881 acctcattgc tgcgcatcat aggccgtatg gcaggcccga ttgtttccat tgctgctgcc
     2941 ggcgctgtta tggcgctgat ggctggctga attcaaaagt accgcttggt accggcggct
     3001 ggagatagtc ccccagtcac gcttcgacag cctatccagt caaagactac ttggccggaa
     3061 ttttaatttt agaaccgaca actaaagtgc tgtctttctt taattgggga ttgagtgcca
     3121 agatttcttc ataccggaca ccgctgccaa gctgcgatgc ggcgattttc cacaaactgt
     3181 cgccctcttt aacaacatat tcaactgctg ctgtctttgg cgctgcgggt gtcaaaccgg
     3241 ctccgctgcc ggcagcagaa acaggcgaag cagaggtgga tggctgtgag gccgcaacag
     3301 cagatgtctg cgcttcttcc tgacttgtct tatccgcttc ctttgccgga cgctgcgctt
     3361 cggcctgaag acgagcaagt tcagctgttg cgcgagccag atcattccgg gcttgagtaa
     3421 tcgtgctatt ggcccggctc aattcggtct tgagccgtac tatttctccc gaagcccgct
     3481 gaagagcatc ctccgactcg gtttttgtct gctgcatctt tagttggagt tcttcgatgg
     3541 ttttggcgct ttgggaaagt ttgggtttca taatcagaac acccgctccc acaccgatac
     3601 cggcacaaat cagtgcgatt atactgaccg ctgctacctg ttgtttcata tatttgcttc
     3661 cttaaaaaag ataataacaa gccggaattg taccctcctt ttattcagaa atcaacagat
     3721 ataattagcg caaattcgtc aatctcaaat atcttctgcc ggccattcaa atctatactc
     3781 ggccgtccat ttccggtcag tcgtgatagt aatgcatccg acagcgctgt agtccgaaca
     3841 cagccaacca agcagatatg cagtcccctc ttcctgaact gacccgacag aaccttcttc
     3901 tgttttacaa tattgtcctc tggcaataaa gaacagacgg cttttgtctt ttcccgcagc
     3961 atcttcgtat gtcttggata cgctgatatt gcccttcatc accgcattga tttggaagtt
     4021 tgttgggcct tcggtccacg taccgttcca ttgggccacg atacggccat ctgtttcaaa
     4081 agccgctgta atccggccgc gcgaagattg attgcggctg acggcagcgg ttctttcaaa
     4141 cggcctgtct atctgggcct tggggggact gggcatagga atactctgtc ccggccctgc
     4201 cagcaggtcc tccagaagcc acggccgctg ctcgaatgcc gccggctggc tgctcttggg
     4261 ttcgggggca acaaaaacat cgatctgcac aaaatacaac agcatcagaa tggccgctgc
     4321 tatcagtaca accagaagtg caaatcccct atattgcttc ttcctgcctg tcatcatcgg
     4381 tttgatgttt cattcgtagt ttgacagcac cctcttggcg ctttcggcac tgataaggtt
     4441 ggcctccagc agcgccccta cggattcggc catggtacac attccgtatc ttttgcccgt
     4501 atcaataact gtacgcagat gatagccgcc gcggtggcgg ataatgtttt taacgccggc
     4561 ggtattaatc atgatttcac acgctacgcg ctgtccgccg ccaatcagcg gaatcagctc
     4621 ctgatgaata atcccttgga gagtttctgc cagcagaaac cgaatggtgt tttcgctgtc
     4681 ttccggggcg tagcttaata accgttctat catcttgtca atggagatga tatgcaaagt
     4741 cgaaatcacc agaatgccgg tttcagccgc tgtcagtgca atcttaatgg tctcgtaatc
     4801 ccgcatctcc ccaatggcaa ttacatccgg atcctgccgc aaagccgccc gcagaccgct
     4861 ggaaaacgag gcggtgtcgc gccccacctc ccgctgacga acaattccct ttttgttggc
     4921 atgcagatac tcaatcggat cttcaatcgt gacgatgtgc cgtttttgat gctcgttgat
     4981 aaaatcaatc attgaagcca atgtagttgt tttgccttgg ctggtgctgc cggtaatcag
     5041 aatgagccct ttttcctttt gcgccaagct cttgacaacc ggtggcagaa acagttcatc
     5101 cggaggccgg ggagtgccgt ccagcacacg aattgttgca ccaaccgtac cattgaaaaa
     5161 acccacatta atgcgaaacc gcgatccgtg ataagccagc atcaggtcaa aatccagctg
     5221 ctgctcgaac ttgcgaattt gttcttctgt cagcatttca tagcatatct ggcggctttt
     5281 gtccggcgtt aaaatttcct ccgtcaccgg tttaagcttg cgcccaatac gaaactgcac
     5341 cggtgcaccg gcacaaatgt gtatatccgt ggcatgctgg gcgatggcaa aatcaaaaag
     5401 ttgattgcgg ttttgcataa gctttttccc attgacaacg ctctaattat actccatgcc
     5461 gacctaaaag acaaatccaa tccatttcaa ctgtttaacc ttcaagatgc atatccctta
     5521 tgccgcatca aagaattggg gctgaataaa ataaaaaacg gctgtctgga attgacagcc
     5581 gtttagaaag tttttgccaa tagttgaatt gcgatcagta aatctccaga attcttttct
     5641 gttattcgtc ggtcttgtct gcctttttgg aacgtttgga ctcggttttc ttccgctcct
     5701 gaacaacttc tgtttcctca tggcctgttt cctttttgcg ggcgggtttt cgggcagact
     5761 tcttcggtgc cggcttctta agcttttccg gctcgacctc gcggattttt gcggtttcga
     5821 gtattttggc aatgcatttt tcttcccgta cctgtaacgc aaactgtgcc agcgatccat
     5881 cgcggataag ttcttcccgc atcttttccg gccgacgccc gcgcagcgca gccacataag
     5941 caatatgccc gttgatttcc tcttctgttg ctgtaattcc gtatttatcg gcaatcttgt
     6001 cgagaataaa atacaacttg agctggtcgg cagcatgctc ttccgaaccg gccttgagct
     6061 gttccatttg ctcatctatc tgctggcgtg tcataccctg catcagcata ttggtatact
     6121 gccgctgcag aatcgagagg gactgttcgg ctactaccgc ttccggcagt tcaaagtccg
     6181 tattctcacg gagataggca taaacttgtt cactcattgc cgcacgtgcc tgacgttcgg
     6241 cacgagccga aagctgctcg cggatgtttt ccttcagctc gttttcatct gaaacgctaa
     6301 accggctgaa gaactcgctg ttcatttcgg caggctcgag ctttttgacc tccttaacgg
     6361 taatagtcag atcgaccttc ttgccgcggt attgctcatt ataaaaagta gcaggcactt
     6421 cgactgtggt tttcttttcg gtaccgctga cagctcccac cagcagcgca tccaaaccgt
     6481 ccacaggcac tgctccaaca aaaccagtcc gacggacaga aatctcaatg ttatcgtatt
     6541 tgtcatgttc aggactgcct tcggtaacaa gaattacatc ggccaccacc cggtctcctt
     6601 ctgcggcagg tccgtctttg ggaatccaca cgccggcctg cttgcgcaag ctcatcaatt
     6661 cctcctcaac gcgtgcatcg gtaatatcta ttttgggctt ttcaatttcg atgccttcta
     6721 actccggcag gtcgaattcc ggacgaactt caacctcaaa ttcaaacttc atcggccctt
     6781 cttccggcag ttcaaccttc tcatggtcaa tatccggatc ccgcagggca tcaattttat
     6841 tatctttcaa ggccgcttcg ctggcttcgg ccatcaattc cagcttgact tgccggctga
     6901 tatctgttcc aaatcgtttc tccaaaaggc gaattggggc tctccccttg cgaaaaccgg
     6961 gcaagatggc ctctttccga agttccttat atttttcatc caatttctcg tgtacaactt
     7021 cggccggaat ctcaatcgca acttttttct tgcacgggcc ggcatcgctg acagtaacta
     7081 cgttgggtat tttttctttg gtttcttccg ttgtctgggt attttcctgc gccattgatt
     7141 tattcctaac tgcttgttat tttgtagttt ataaaaataa aaaaggccgg cgaacacaat
     7201 tcgacgacca aaagcatccc aaaacccttt cgggctttac aaacgcttat gcgaccagcg
     7261 ccaggccact ttcagccgat acctcgcaaa agctatgtca atttttcaag tacttcttta
     7321 acgctttaca acacttaaat tcgtttcaaa aaagcgggtg atgagattcg aacccacgac
     7381 attcacgttg gcaacgtgac gctctaccac tgagctacac ccgcattacg aaccccatac
     7441 tatacatact cctgcaccaa tttcaacccc caagcacaat tttttgagat ttttctttct
     7501 ttaagtcctc taaacagcct ttgccccttg caggggcaat tttttacttt atcgaaggta
     7561 aaaacttgaa aagccaaagg catcccatcc ctaagccgaa aacgcataag cagggaatag
     7621 cacatatatt ggtctttacc tatccgctat gtctgcatac tatataatct aaccaattaa
     7681 aaaatcacaa aggcttcaaa ttggcataaa tgacgccgtt ttcgaaaagt ttgggaactc
     7741 ttccagaaac actttggccg cttgtttaca ccccatacac atattttcaa tcccacaaca
     7801 aaatcaaatt caattggaca ataattgttg ttgaaaaggc agctcatgct gtataatcag
     7861 cgtgtaaaat ggatgttacc ggaagggcag cattatggtc aaaccgtttt ttgatctgca
     7921 ggcacaccaa accacggtta aaacggaagt tatcgctgga tttaccacct ttttgacgat
     7981 ggcttatatc atctttgtca atcctaatat tctggccgaa gcggggatgg ataaatccgc
     8041 cctcatcgcc gttacctgcc ttgttacggc cgtttccacc atcgccacag gccttttggc
     8101 caatgctccc atcgctatgg caccggggat gggactgaac gcctttttcg cttatacttt
     8161 ggttctaacc cgtcaagtta cttggcagac cgctttggcc gttgtatttt tatcgggttt
     8221 attctttctg attctcacct tggccggact tcgcaaaaag ctggtggaag ccatccccca
     8281 atcgctgact gccgctatct ccgtcggcat tggactgttt atcaccttca tcggtctgca
     8341 gaatctcggc cttattgttg cgcatccagt aacgctggta acttcggcac acttaacgcc
     8401 taccattctt gtgggtctgg ccggcttggc tgtcatgata atttttgaaa tgcggcggat
     8461 tcccggtgcc ctgctgattg gaattgcatt ttcaaccgct ctcgcggcgc tgttggggta
     8521 tatcagcctg ccgcagaagc tggtttcgat taatataaac atcctgccga cagcattcaa
     8581 gatggatttt gtcggtgctt tgaaatgggg gatgttttcg agcattttta cgctgatgtt
     8641 tattgatatg tttgacagca tcggcactct gctggcggtt gccccgcagg cgaagctggt
     8701 agatgccggc gggaaattcg agaaactgga tcggctgttg accattgatg cggccgccac
     8761 aatgtttggt gctgtctgcg gcacatcgac caccacctct tatatcgaat ctgcagctgg
     8821 aattgcgcag ggcggccgga ccggcctgac tgcggtagta acagggattc tgtttcttgc
     8881 agcaatgctg tttgtgccgc tggcaggcat tgtgcctaat tatgcaacag cgccggcgct
     8941 gattatggtt ggcctgttta tgacgcggca gatacgcctg attgattttt ccgatatgca
     9001 gcagggactg ccggcattta ttattatggt gatgatcgct ctaagctact ctatcagcac
     9061 aggactggct ttcggtttca ttacctatac gctgattgag atcctgacgg gaaaattccg
     9121 taacatcaag ccggcaatgt gggttattgc cgttctatcg gtgttatatt tctgcgttta
     9181 gacaaattaa agagtaaaac agttcggatt ttaatcggtg ttttgctatt ggttttatgc
     9241 aaatcttggc ggattttttc ggattttaag acgcatgaaa gcaaaacagc agggaggatt
     9301 ttttatgatg ctgaaggtta caagttcggc atttgagcac aatggaatga ttccggctca
     9361 atacaccgcc gacggcgaag atatttcacc tccggtttct tgggaaggtc tgccggaaga
     9421 tacgcaaagc atcgcgttga tttgtgacga tcccgacgct ccaatgggga catgggtgca
     9481 ttggctggta tggaatatcc cgccgcaaat gacagggctg gctgagcgtt tcccgaccga
     9541 tgctcagtta gacagcggga ttcgccaagg cataaccgat ttcggccaaa gcggttacgg
     9601 cggaccggcg ccgccgtcgg gcacacaccg gtacttcttc aaagtctatg ccctcgatac
     9661 aatgttaaat ttgccggcgc aggcagacaa gcgtcagctg gaaaaagcaa tgagcggtca
     9721 tattctggct aaaggcgagt tggtgggcaa atactcccgc agacgctgat aaggtgcaga
     9781 actaaaaagc cagatagggc ttggcttttg ccaagagctt tccggccagt ccaatatctt
     9841 caatgcagca gatgcggcca gagcggcgct gttggaggat gcggttgacg gctgctggac
     9901 ctagaccggg tacacggaga agctcgaatt tatcggcatg gttgatatca acagggtaaa
     9961 aatcggggtg catctttgcc cacgtcgttt tgggatcttc agtcaacgaa agctgcccat
    10021 gggcatcgaa aagaatctcg ctgtccttaa aaccgtattt tcgcattaaa aaatcaacct
    10081 gataaagccg atgctcacgg gtcaggatgt cctctcgact ggcggcctgc tcggcaggca
    10141 aatccggact gcccaaaccc tgctgatagg cgctgaaata aatgcgatcc agtttcagcc
    10201 gctcatacag gccgcccata tatcggacaa tctgccggtc cggttcaccg gcagccccga
    10261 cgataaactg cgtcgtttgc ttaaccctgt gccagcggga cccccgggca gtcaatctgc
    10321 tgatatgctt tattggctcg ataatgtccc gtatataatc ttttcggctg gacactttgg
    10381 acaaatattc ggcacccggg gtttcaatat taagcgatac cgccgaagcc agagacacgg
    10441 cctcattgat agccggaatg ctggcaccgg gaataatctt caaatgaata tagcccctaa
    10501 aggcatgtct gttgcgcagc agacgggcaa tcgcatcgat gcgttccatt gtggaatcgg
    10561 cagtaccgat gactccggaa ctcaaaaaaa gcccgaaaac cttgccctga ttataataat
    10621 ccagaaacag gcggactatt tcatccggcg acagactgca tctgcgtatg tcttgatttt
    10681 cacgcagcgg acagtatttg cagtcgttgg aacagacatt ggacatcaat accttaaaca
    10741 aactggccct gccgccgctg gcgagagtta tagggtaaat ccatttgccc tcgctgctgc
    10801 gtctgcgatg ctcatctttg gttgagccgc aggcacaggc caagtcatac tgggcatcag
    10861 cagagagaat cttaagtttt tcgatgatat caggtttgcg gacaattact gtcattaatc
    10921 tattttatcc caatgtctgc accttcattc aacttgctct ttgtaattct atcggcattc
    10981 cgccgtcaaa agcaatcccc gatgcggcag tttttcccat tttttccggg ctttttacaa
    11041 ccatcacaaa ttactgctga atcttgtatt tggagccatt ttgtgataaa aggagctatt
    11101 gtaactgaat atagatggaa ccaatttaag gacagaaaaa tgccaaacac cctgacctat
    11161 aagattttac agaagcattt agttgaaggg gaactgaaag ccggtcaacc tattggaata
    11221 cgaatagacc agacgcttac tcaagatgca accggcacga cagcttttct gctgtttgaa
    11281 tcaatgggaa ttgcccgcgt gcagacagag ctttcagtca gctatgtgga tcacaatatg
    11341 gcccagttcg ggccggaaaa ccataacgac cacctctatc tgcaaaccgt tgcagccaaa
    11401 agcggtgttt accattcgcg gccgggcaac ggcatttgcc atcaggtcca tctggagcga
    11461 ttcgcccggc cgggagcgac actgctggga agcgactctc atacgcctac ctgcggcggc
    11521 atgggaatgc tggccatcgg ggccggaggc cttgatgtag cagcggcaat gggtggcggg
    11581 ccgttttatc tgacctgccc taaagtgatt ggtgtagaac tgaccggccg gctgccggac
    11641 tgggtcagcg ccaaagacgt gattttgaaa ttgctgaaca tcctgacgac taaaggcaat
    11701 gtcggctgtg ttgtggaata cttcggcaga ggcgtccaga cgctgtctgt gccccagcgg
    11761 gccacaatta ccaatatggg cgccgaactt ggtgttacca ccagcatctt ccccagcgat
    11821 gagcgcacct tggagtttct ggaacttcag gaacgcgacg atgtctatgt tcctctgtcg
    11881 gcagacccgg aagccccgta tgacagggtc attcaaatag acttgtcagc gctggagccg
    11941 atggcggcct gcccgtcatc gccggataat atcgcgccgg tttcggaaat tgcgggccgc
    12001 aaggttcatc aggttattat cggaagctgc acgaattcca gttatatgga tgtgatgatg
    12061 gtggcaaaga cccttcaagg ccgcaaaacc gacccaatgg tcgaatttgc cattgctccc
    12121 ggaagccgac aggtactgga aatgatagcc gacaacggtg ctctgacaac cctgatagcc
    12181 gctggtgcac gcattctcga atcggcctgc ggaccatgca tcgggcaggg attttctcct
    12241 gccgacggca cggtaacctt gcggacattc aaccgcaact ttgccggacg taccggaaca
    12301 cccggcgacc agtgctatct ggtcagccct gaaacagctg tggcttcggc cattatgggc
    12361 gagattaccg acccgcgcaa tttgaaaaaa atgatgggca ttgcttatcc gcgaatcaaa
    12421 atgcccaagg catttctcat tgatgacagt atgattgaaa aaccggtttc tttagacgaa
    12481 atcagccggg ttgaagtggt gcggggaccg accattgtcg tgccggaatc accccggccg
    12541 ctgccggagc atctgaccgg acaggtattg ctcaaatgcg gcgataaaat tacaaccgac
    12601 catataatgc ctgccggtgc atttttaaaa taccgctcga atgtccccca gtacagcaaa
    12661 tacgtattca actgctttaa cgaggaaggc aagccgacct ttgccgaacg ggcaatggcc
    12721 ttaaaaaagc agggtattgg cggcattgtc gcagccggcg aaagctacgg tcagggttcc
    12781 agccgtgaac atgcggcctt atgcccgatg tatctgggtg ttcgagtgat tttggccaaa
    12841 tctattgagc gtattcataa ggccaattta attaacttct gcattgttcc tgttgagttc
    12901 tccaatcccg atgattatga agcgattcac cctgatgata ttgtggaaat tccagatctg
    12961 ctggccgcca ttaagctgag ggacacactt gttgttctga ataaaacggc aggaattgaa
    13021 attaaaggca aattgaccct gtcccaccgg gaccgggata ttctgctggc cggcggactg
    13081 ctgagctaca cccacaagaa agcagtagcc cgatgagtac cagcacgatt attaccgtct
    13141 ttggcacaag caaaacccag ccgggcgagc cggagtttga aacggctaag atactggggc
    13201 ggctcttggc aaaaaaggga tttaccatcg ccaacggcgg ctacagcggt acaatgctgg
    13261 ctacagcaca aggagcagcc gaagcagacg gacaggttat cggcgttacc tgccgggcat
    13321 tcaaacgcgg caaagccaat ccttatatca gtcgggaaat tcagacaaac agcttgaccg
    13381 agcggcttgc tatactggtt gaactgggca aggcctatat tgtattgccc gggggaacgg
    13441 ggacgctgct ggagctggca gatgtctggg aacataaaaa taaaggattt tcaaatgcag
    13501 aaaaacccat tatactgatg ggggcatttt ggaagccgct gctggaaatg atgggccggg
    13561 cagatgccga aagtctgcgt tcggttgact atgctgaagg tcccgaagag gccgcagcga
    13621 taataacggc aaaactcagc acaaagcagg tcgattatgc ctaaaaaatt atattgcttg
    13681 ttttttataa taattttcag ttttgcatcc ttggctgctg accaagatgc aaaatctgcc
    13741 gtcaaagaag ctgttggggt cagtgtagtt gatgacggct ttcttgccga cggagtcgaa
    13801 ggctgggcca aaaaagagac ctcttctgga gtgtggtctt ttgtgccgga aaaaatgatt
    13861 gatgccggcg gaggaaaagt tattgcttcc ggacagccgg tacagatgct gccctgtgct
    13921 gttctggaac agatagtcaa actggctggg caaaacaaac aactccagat tcgcctatgg
    13981 gctgtgttta cgaaatacaa atatgcaaat tatctttaca gtgtctattt tctcccgatt
    14041 caagaccaat cccccaagcc accggcggaa agccaaacac ctgctgggac gcagcacaaa
    14101 gcggcacagc cggaagattc gattattcct gaagaaatac ttgcccagat taaatcaact
    14161 cgaacacctg atttgaaaaa attccagcag attgctcagg taactgggga cgttaatctc
    14221 ataggacgga caggttatct gattgaacaa aacggcaaac ggtattttca gccagatgga
    14281 ttcggaatga agattgacag ccagccactt ctgctgctgc ccaatgaagc ccgtcagaca
    14341 accgaacggc agcttaatct aacacccggc cggcagcgat acagtgtttc cgggctgatt
    14401 acaacattcg aggggcagcg gtatctgctg cttcggaggg ctatccggac ttatacgcat
    14461 ggaaacttta ccccctgagc tgagatcgat gaactttgac agtattctgc ttcgaacaac
    14521 ggatgccgat tatgcccagc ggcttgagcg tttgaaggcc gaacactcta tgggcggttt
    14581 tctccaaagc cgcggtcagc tgattcaaac tgttcgcaat atcgttgctg atgttgccaa
    14641 tcgcggcgat gcagctgtgg ccgaatacac cgaaaaattt gacaaggtca aactacaacc
    14701 ggcagagttc agaataccgg cagaggagct gaaaaaggcc tacgagcagt tggacaggcc
    14761 gctgcttaaa tcgctcaaaa aagctatagc gaatgtgcgg aaataccaga gcgacatatt
    14821 tatcggcagg aaaaaccgtt atcccggcat ccgctatatg cctctgaaac gtgcggcgct
    14881 gtgcataccc ggcgccagtg cccccctgcc cagcactgtg attatgacgg ccgtgccggc
    14941 tcaagcagca ggtgttcaag agctggctgt tttcagtccg ccgcgctata atggcagcat
    15001 tcaccccgtt attctcggcg tgtgctgtga attgggcata gcagaggttt atcgcattgg
    15061 gggggcacag gcggtggctg ctgcggctta cggaacccaa accatccgca aggtcgataa
    15121 gatcgtcggt cccggccatg atgtagtgca gctggccaag aaagaggttt tcgggctggt
    15181 tgatatcgat tcctttgcag gccccagcga tgttctgatt gtcgccaatg acaaggccga
    15241 tccggcatgg gtggcagcgg atatgctcag tcaggccgaa cacaatcccg gtgcagggct
    15301 ggtcgtaacc gattcactgg actttgcacg caaggtcttg gctgaattgg gcagacaatg
    15361 tgccgcctta gaccgtgccg aggccgcagc cagatgcctg atggattaca gcgctgttgt
    15421 tgtgtgtgaa tcaatggagg cggttattga ctgggccaat gattttgccg ccgaacactt
    15481 gcaggtgcag tgcggccggc aaagcaaggc tatcagcaaa aaactcatca acgcaggagc
    15541 catcttcatc ggcccttatt ctccggtcgc tgtcggggac tactgggcag gtcccagcca
    15601 tacactgcca acccgacaga ccagcaaatt cttcagtgca ctgaccagca acgactttgt
    15661 caagtccacc agcattatcg aatacaaccg gcagcagtta gaagatgccg ccgatgacat
    15721 tgtgcggctg gcccggatcg aaggtcttga cgctcacgcc aaaagcatcc agaaacggct
    15781 gaaaaggccg taatcttttt tcccccaatg ggtagaagta acggcggaac aacttatccg
    15841 tcactaacct gatagcctgc ctgctccatg tgaatatgct tcttaagccc tttggcagac
    15901 tggaaggcat actcgcggtc tgcctgcagt accacagtat gcccatcgac agtcagattc
    15961 aatgttagtt caggctggcg ctgatgaata atattggcta atgtgattgc tttttccagt
    16021 ccgtcgccgg tattgtaatt ggcaacctca tccggctggg caagacgcgg gccttcgtaa
    16081 atcgactcat ccggcagact gcaaagctgc tgatacacat ctggaatatc agaatcccga
    16141 tatttttcaa tggaaaccgg cgatcgctcg agggctgctt tcatgaaggg tctccaatcg
    16201 caccgcgtca tatcgcgata ggcataaaac gccaaatcgg ccgtgatgtt tctggaacgc
    16261 atggaatcca aatactcggc aatctgctgg cggttccact ccgggctcag tttaatcggc
    16321 tcggtttttt gatatgtttt atccacagcc ggcagcttgg gctcaatttt gagaaaatca
    16381 gccatttcac taacacaggc ttcggcgtcc tgaccggtat agggggcaat aaaccgagcc
    16441 agcttcagcc gatcagcaga cgattgaata tcaatctttt catactggat aaaggccatc
    16501 agctgctcgc agcagatacg gccgggatag gggtaaacca taaagtcatc ttctgagact
    16561 tcttcaagca gtttttcaaa ggtctcatca taaatccgaa acttgctgcc atgctcatag
    16621 ccgaaaagat tttcggcctt tacaaacatc cgctcgcctc gccgatgccg gcagaactgg
    16681 aaatatttct gatatttgcc gcgtgagcgg agaaaattgg caaggttgag atgattcagc
    16741 tccgtttgga gataatcgga cagccgccgg cagaaatgct gatagcgctg ggggtcgatt
    16801 gtggcctgct ggtaaaagca gtggatataa cctgtatgat gcgcaacaat agtaatctgc
    16861 tcgttgcgaa gcgcccgctg ggccttgtcg gaaatctctg tgccgttaaa ccacatcgat
    16921 tttgtaacca gccggcggtt gttggtaata ataccgtcgc tgacatcaaa aaagttctgg
    16981 gagtgcagcg gcgtcagcac catatagata tcttccagac taagtccaca gacaataaaa
    17041 gcagccgctg catacagggc cgaaagagat acacactccc cagccccgac agcataccct
    17101 tctttgcggc ggaaggcgtt catggcttcc acagtttctg ttttccagta gggaatcaca
    17161 tcgccgtcgt attggtccag cccgaagtgg cggcgtatat ccacatcaaa ataataggaa
    17221 tcgcccgtat agccgaacag ggcattgctg cgggcgatgt catcgggaga caggaaatcc
    17281 ttaaagcggc ttaattcaac atccttgcgc gtcagtcccc atgtttggag gtccagatta
    17341 aattcatatt catccattac atactggcgc atgcgcatca ggcggcgata gatatttttg
    17401 ccttccagtt ttttgaatgc cggatcctct cgccagcgcc agataaccgg actcatgata
    17461 ttggccagca ccagtgcata catcagctcg ggaaaaacaa acacttccat atccgacagg
    17521 gtaatcgccg agctgtattt ttccaaatcc tgctgattca taaaacctgt tcctttatcc
    17581 aaaatgaagc tcgataatct ccttgtcgga aagcacatgt tcgcgcggca cattctggcc
    17641 gtcgtgaata cccggcgcat tccaagcgcg ggcggtttta agtttttcag ccaactggcg
    17701 atggatatga caggccagat catacactgt tgcgccgcgc ttgagcgtaa agggttcttt
    17761 gaagtcgggt tcggcaccgg gttttttggc ataaatccgc accacatcca gcatttcaaa
    17821 cagacgccgc atcagggtat caagcccctg ccgcagttcg gctgatacgg caatatactc
    17881 aaacgggcgc tgggttaatt ccttgagtgt ttccagcgtt ccgggcggag cgatatcggc
    17941 tttcgctgcc agcacaaatg ttttgcgccc cagacgatac tcccggctgt cgggattggc
    18001 cgccgcttca tcgagaatca attcgtggga atccagatac cggaggcaga cgtccatctg
    18061 ttccagaaca tcggcagata aatcaataac aatcgccagc aaatccgccc cgcgcagggt
    18121 attgacaagt cctgaggggg cataatcggc agttatcgga ggggtatcaa caagctgaat
    18181 cggaacatcc tcaaacatcg ccattccggg cacgggcttg tctgtggcaa acggatacgg
    18241 tgctaccgtt accggagcat gggtcagggc tgccaaaatc gagcttttgc cagcattggg
    18301 cattccaatc aaagcggcct gtccagcacc gctgcgggga atgtggaaaa tatcgccgtg
    18361 cctgctgccg ctccttttgc tttcgctttc cagcgcctct ttcaatttgc tcattcggcg
    18421 cttgatatcc gcctgcatat gctcggttcc tttgtgcttg ggaatctccc gaagcatttc
    18481 ctccagcgcc aagagccgtt cctggtctgt agcggcggcc ttatactgtt cttcggcctt
    18541 tttgtatcgc ggtgtcagat ttgcaggcat atcgtaattg tcaatgaaaa atgtcagatt
    18601 actccattga ttcggctact tttctattat atgaaaaaaa acctaccgtg taaacctgca
    18661 ttgaacaaaa tatcacaaat cagccgagac tttatctcag aataatctct tccggctggg
    18721 aaagacaatt ggggggaagc attcggagca gttgattaac gaacgcgtgt gtgccgatgg
    18781 ccgccggcgt atgggcatcg gaaccataga ccagcttgac gccggcgtcc aacgcaatct
    18841 gataaatccg gtgccactgg ctctgccagt atgccggcag acggctgccg gtcagttcat
    18901 ttaattccca agcaatatga ttacgggctg aaatctctgc ggctttgcgc atcacttgca
    18961 agccgtactc aaaatggata tccaagtcca tacaagccgc cagcatgcga ccgggatggg
    19021 ccaatgtatg aaccgccgga ttgctgacga ttcccagcag cgaactttcc cagacctgca
    19081 gaaacgtttt ttcatccagc cggcggtcat cgggcgagcg ggggtagtgg ccttgggtcg
    19141 gcacataatg gaagccggca atcacataat ccaatccctc gaagacatcc gtaaccagcc
    19201 gtccatcggt atagtcggcg tctacatcta tctctgcacc gatatagacc ctgcagcgcg
    19261 gctgacaagt cctcacttcc tttcggattt gctcaatgcc aaacatatca gccggttcgt
    19321 aaatatggtc ggtaattgca atcgctgaca gctgcagctc atcagcccgc cggcagatga
    19381 cctcgaccgt catttccggg gcatgaccgc aatattcagt atgaatatgc aaatcgtaac
    19441 caatcatctt tttccactca caaacgccaa tatccatgcg gccagaaaac cgactggcac
    19501 acagaacaaa atcggtcccg ttccgaagcg atgagcggcc ggccccagca gcatcggcaa
    19561 taggcaggcc gccagccaga taccgccgac aatgaatccc atccgtctgc ccaagttcgg
    19621 ccccctgctg ttacgggcgg cgctgatcat cagcggatag gccccgtagc agaaaaatcc
    19681 gcccacgcaa agcagccctg cggcccaact tctgtctgca agcagcagat acgctccaac
    19741 aaagggggta ccgaccgcca acatccattc aattaatttg atttctccat accggtcggc
    19801 ccatcgtgcc acaataatgc cgccgatgcc cccggccatg ctgaacagcg acaccgccac
    19861 tccgccgacc gttagatccg taccgatttc attaagccgc tgcggcacaa tccacattag
    19921 cacctgtaca gagcaggctg ataaagtcgc aatcgccatc accagccaaa aagaaacata
    19981 cagattggag ctctgcgccg ccctgcggga agcttcatcg ctttctacag ccagccgaac
    20041 acgaaagcaa agcgtcagca ccagcactcc aagcgaagcg gcacaaaacg gaattatact
    20101 gaaaagtccc cacatcttaa acaagtgggt gctgccccaa cctcctgcgg caaatccgat
    20161 aacgcggccg gccataaaaa ccgctgaacc gagggccgaa gaaattccat tgagccgatg
    20221 cacggcccgc agagcttccg ggtgagtcat tccaacgccc aagccgctca gcacagcaat
    20281 cagcgaaagc caaactagta ctatggaatt tttagggact gccgtaaaca gcagaatgga
    20341 acaaaccaga attaatccgc catatagaaa gaagggctgc tgccggtcgg ggcgcaaatg
    20401 gccgataagc acttgaatgc cgttggctga tacgagaaat gcggtcagca gaatcccgcc
    20461 ggcggccaca cttaaatgaa acgactgctg aagcccgggt aatacggtgt gcattaaacc
    20521 ggggaatgca tccagcacga aatgagccgc tgtcagcacc atcaactgca tccattgctt
    20581 atgctgctgg tcggtgtgca tagtcagttg tcaatccgca gcaggaattc ggcgccctgc
    20641 ctgaaaacac tgcaggggta atcaaacaac tcactcatcc ggcggctggt aataacatcg
    20701 tcaggctcgc caacggccaa aacctgacca tttttcagca ataaaacctg ctgaatccaa
    20761 ggatcgattt cttcgatatg atgcgttaca agaatgattg tgggggcatt gtcctgccga
    20821 gccaacactg ccaaatcatt cataaaccgc tgacgagctg cgggatccag tccgatgcag
    20881 ggttcatcca aaatcagcaa tttcggctcg cacaccaatg cccgtgctat cagcactttc
    20941 tgcatctcgc cttgggataa tgttccataa tcctgatggg cgatcgaatc ggcacggagc
    21001 tttttgagca tatccagcga tttggcaaat tcttccgggc tgatttggcg atacaatcca
    21061 atcgaggcat ccagtccgga ggcaaccacc tcaacagcca gatcctgcat aggcagccgc
    21121 tgggtcatcg ccgagctgac ccacccaatg agcttccgca gccggccgat atcacagatg
    21181 ccgaatcgct cgcctaaaac acgaactgtt ccaaacgtcg gccactcgta gccggtcaat
    21241 attttcagca atgtcgtttt gccggaaccg ttggcgccga gcaatgccca gtgttttccg
    21301 gcttcaattc gccatgacac atcggacaaa atcattctct ttttacggca aaaggtaact
    21361 ttatccagct ctacaaccgc aggcgtcatc tattccgccg gaataaattt cttgccggtt
    21421 aaccgctcat aggcctcaat atatttggcg ctggttttgc tgataatatc gtcgggcaaa
    21481 acaacaccgg gaccagattt attaaagcgg atttcttcga gataatttcg tacaaactgc
    21541 ttatcaaagc tttcctgttc gcgtccgggc tggtatttat ccgccggcca gaagcgggat
    21601 gaatccggtg taagcacctc atcaataagg ataatccggc cgtcaatcaa accccactca
    21661 aattttgtat cggccagcag aatacctttg gatgcggcat attggcctgc tttggtaaag
    21721 atttcgatgc tcttgtcgcg tatatattcg gccgtttccc tgccgaccag tttgacacag
    21781 gcatcaaaat cgatgttttc atcatgcgtg ccgatttccg ccttggttga gggagtaaaa
    21841 attgcttccg gcagtttttc acactgtttg agtccttttg gcagtttgat tccgcagacg
    21901 gtcccattct gctggtattc tttccaccct gagccggtaa tatagccgcg aacaatgcat
    21961 tcaacaggta gtactttggt ctttttcaca agcatactgc gtcctgccaa ctgctgcgga
    22021 taattagaaa acggggcagg aaaattttct acattatcgc tgataaggtg gttctcgacc
    22081 acgccagtca agaaatcaaa ccaaaatttc gatatttggg taagcacttg acccttatag
    22141 ggaattccac acttcataac gacatcaaaa gcgctgatgc ggtccgatgc tacaatcagc
    22201 agtgcatcac ccaagtcgta aatatcccga accttgccgc gtcggcattg gactccttta
    22261 atgtctgtct gtaatactac attggccatc gcctaactcc agtactgata tgtgatacct
    22321 tttgtgtatt ttcacgaacc gcacgcctta ctatattcaa taatccagaa tatgcaagtg
    22381 caatctggct taacacaaga gaaacaatct ttccagcagc atgaagccgt aatgttattg
    22441 tctatgccga taaccaatcg agctgaaagc aaagaatttt gataaatttt tataaaaaca
    22501 caagagacaa tctattctgg atgattataa tacgtttatg ctaagacggc ataattgagg
    22561 atgaagcata aatcaaatat tgtattaatc gggatgccgg gcagcggcaa aagtactata
    22621 ggagttcttt tagccaaaag actgggtatg tcttttctgg atacggatgt actgattcaa
    22681 gcccattggg gacggagtct gcaggaactg attcaattgc acagaatgga gggtttctgc
    22741 cggattgaac aggagtatgt gcgttcgata aatgcggtaa acaccgttat tgctaccggc
    22801 ggcagcgtcg tgtattataa ggatgcaatg caagcacttg gagtaaacgg cttgattgtt
    22861 tatctgcgac tgccgctgga gcttttacaa aaacgcttgg gggatttgaa cctgcgaggc
    22921 gtcgtacttg aaccgggtca aacgctggcg gctttgtatg ccaaacggat accgttgtat
    22981 gaacaatggg ccgatatatc cgttgacttg aacggcctta atcacgaaga cagcgtcaag
    23041 gccattcttg ataagctgaa tgcagcaaaa cgaaaggaac attgataatg aaactggaaa
    23101 aatattcgat gggtataggc gaccgcttcg gtcatcaggg caaggctcag ttatcagcac
    23161 tgataacggc taaatccgaa ggcattcatg tcatcccggt ctggaataaa tccaaccggg
    23221 aacacagcat cattggcaca gtacctgccg atacacgtgc ggaagcggaa gctgcaacgg
    23281 ccgctatggg ctggacagat gcctattatg tggatgccga ccatatcaat ctgaataatg
    23341 taaccccgtt tatcgcctgc agtgattttt ttacattgga tgtagcagaa gctatcggca
    23401 agccctgtga taaggccgca cttgacaaat ttgtccgcag gcacagcaac ctgacaggac
    23461 agctgtcttt tgacgggatt gagcagactt ttgatataac cgaggacatc atccgtcgca
    23521 tcggctcaaa ataccttttc gccgttcagg aagccggccg gatttatcgc catctggtct
    23581 cggccaaagg acagggaaat ttcattacag aaatttcgat ggatgaaacc gacacaccgc
    23641 aaacaccgct ggagatgctg tttattctgg cagctgctgc cgatgaaggc atccctgtcc
    23701 aaaccatcgc accgaaattt actggccgct ttaataaggg tgtggattat gttggtgata
    23761 tcgaacagtt tgaaaaagaa ttttttgcca atgtagcggt cattgcatat gcgattcgcc
    23821 agttcggcct gccggataat ctgaagctga gcgtccattc cggcagcgac aagttttcca
    23881 tttacgaacc gatacgccgg gcgctgaaaa aattcaactg cggccttcac ttaaaaacgg
    23941 ccggaacaac gtggctggag gaactgatcg ggctggcaac cgccggcggc gatggcctaa
    24001 agattgccaa agaggtgtac gccaaggcct atgcaaaaat ggatgactta tgtgcccctt
    24061 acgcaaccgt cattgatatc gacaagcaca tgctgccctc acctcaggag gtttacggct
    24121 gggacgggct gacctatgcg gcagcactga agcatgaccc gaaatgtgaa ttgtataatc
    24181 cgcatctgcg tcagctcttg catgtcggct ataagattgc cgcacagatg ggcaaccgct
    24241 accttgacgc tctggccgag tacgaagata tcgtcgctgc ctgtgtcaaa gagaatatcc
    24301 tcacacgtca tatccgacga atctttattt gatcggcccg ccgatttcaa caattcgtac
    24361 ttcgtaaaat gtttcagcaa tgcgataaaa atctctctgc gctgctaaaa tgttaactcc
    24421 aattttggag ggggcaaatt tgctgaaagt tctatattta tcgcggcttg catctctcat
    24481 ccatcggccg gcgacaaaga aatgaagtca ttgcaagtcg atgctaacgc ggcctaaagg
    24541 gtgccatcct gtcaaagtga aaatcgccag aagtgcaaaa atggcagaaa taagaccttt
    24601 tctttcctgc ttgtaattac tataagatat tgcatataaa tgagttatag taaattttta
    24661 ttttttggca cagcatttgt aatattgaaa aacgagtcgt aaaaaagaaa aaaaacaacc
    24721 atttattgaa agaaaggggt gagtgctatg gtctttcacg aaatggtacc ttggaaaaaa
    24781 gacagacgca acgttaaagt ccgaactgaa caggatgaat ttcctctgat gagtctgcaa
    24841 cgccgcatga accgattgtt tgacgatttc ttcggtgatt ttggtgactt ttctctcagt
    24901 cctttccggt cactggcacg cggagcaaat gaatttatac ctcggataga cgtgacggaa
    24961 aacgacaccg agattacagt taccgctgag ctggccggaa tggatgaaaa agacatcgaa
    25021 atcacacttc aggacgacgt gctgaccatc aaaggggaaa agaaggccga acgcgaagaa
    25081 aaagatgtca atcgctatct gtgtgaacgg tcttacggct cgttctgcag agcgattgag
    25141 ctgcctgcgg agatacagca ggataagatt gaggccacct gtaagaaagg aattttaacg
    25201 gtacgtcttc ccaaaactcc tgccgctcaa tcgaaagcca aaaagattga ggtcaaaggc
    25261 gaataactac acaggggtca tggcagccgt ttggctgtca tacccttttt tgttttttcc
    25321 cgttatttcg acattctata ttctgtattt tctgaattct gcacaggaat ataaaaaaat
    25381 aattctttct tctgagggtg gatggtataa tgtgcactca cggatgtcca gcttaatgtc
    25441 taatagcgat gctcatgcga attatttatc agattttgaa caaataacta ttgcgaacaa
    25501 tctgtaatgg cagacaacaa tactataccc aatggcatat cttcattgcc ggttaaatca
    25561 tttatgacag tcggtcccac cctgcattac agtcattccc atgtacacgc catgtgggga
    25621 ataaccgttt ttgtatttgt catcgtttgt tatttttgga atcagattca aatcggagga
    25681 ccagtatcga ttgatccgat ggagctggcc gatacatcct tatttcgttt aggccaaatt
    25741 gtcgtacgcc cgatcagtat ttatgagtat ccgtggcata ttattgtctt gggcacatta
    25801 atgggcattc tggcagtcgc tccggtattg gtctcgcaat tatacagctt ccgatattcc
    25861 cttccgctgg tgctgagcat cttttttatc ggacagctct atttgttcag cggatttgtt
    25921 ttggtcagct gccttgcagc ggcctgccgg ccgctgcggt ttcggtcccg tattgtcgcg
    25981 atagctctgt gcatggctcc gcagcttatt tactgggcgg tttggggagg atatccaact
    26041 gcagatccgg tgcggtgggg cttttcattc gccccttgga tttatgcctg gctggcggct
    26101 ctggc
//
LOCUS       DAOTBO010000029        25983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1554_length_25983_cov_5.533400, whole genome shotgun sequence.
ACCESSION   DAOTBO010000029 DAOTBO010000000
VERSION     DAOTBO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 25983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25983
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1554_length_25983_cov_5.533400"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /gene="atpA"
                     /locus_tag="PLE88_05300"
     CDS             <1..1373
                     /gene="atpA"
                     /locus_tag="PLE88_05300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOM75917.1"
                     /translation="MLQFDGGVVGEAFNLEEDSVGAIIYGDYTKVKEGASVRATGKVL
                     SVPVGEALLGRVVNALCEPIDGKGRIAAESRRLVEFPAPGIADRQPVKEPLATGIKSV
                     DAMIPIGRGQRELIIGDRKTGKTAIALDTIINQKGKGVVCIYVAIGQKESTVAEVVAT
                     LARHGAMEHTIVVSASASESAAMQYIAPYAGTAIGEYFMYEKHGHALCVYDDLSKHAI
                     AYRELSLLLRRPPGREAYPGDIFYLHSRLLERACKLSDAKGAGSLTALPIIETLEGQV
                     SAYIPTNIISITDGQIYLQRSLFLAGVRPAIDVGISVSRVGGAAQPKAMKKVAPKLRL
                     DLAAFRELQDFARLGTELDEAAQKQLERGYRMVEVLKQKQFAPMSVAQEVLSILAGSS
                     GALDDIPVTRVNHFMDELLSWMQIEAPEYLESIMQKGDIDDALMTGLTEAIEAYKTIY
                     RFSFGA"
     gene            1492..2394
                     /gene="atpG"
                     /locus_tag="PLE88_05305"
     CDS             1492..2394
                     /gene="atpG"
                     /locus_tag="PLE88_05305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOM75918.1"
                     /translation="MANTRKILQRRNAAANISKVTGTMETISAVRYRQYYHQWSEGLA
                     FYDSLAQLAYLMVTSQSKIQHPLLEEPKIKSKTNALIVIGSDRGLCGAYNNEIYRQID
                     THIRMAKRFGRQLNIYAKGKKVLAYLEHLKITPAGIYDEFSEVPTAEQANKIGDFFIE
                     AFLKGKIDRLGIVYNRFFSPASQKAQTLTILPVVDLIDDLTTRATVIWPWELNFEDFE
                     LSPSPEDIFETLSKMIIRTSIAGCFLEAAVSEHLSRVVCMRNASDNADEMIRELTKDY
                     NRARQGQITVELLDIVSGVEAAKR"
     gene            2416..3831
                     /gene="atpD"
                     /locus_tag="PLE88_05310"
     CDS             2416..3831
                     /gene="atpD"
                     /locus_tag="PLE88_05310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOM75919.1"
                     /translation="MNRGRITQVIGSTFEAEFAAQVPAVYNALEVKGEFEGGRIDLVG
                     EVQQHLGGGRVRAVALGSTAGLRRGMEVIDTGNPLMVPVGKETLGRVFNLLGRPVDGG
                     PDISVKEKRPIHRKPPKFTALTAKSEMFETGIKVIDLLCPFVKGGKTGLFGGAGVGKT
                     VLIQELIARIATQHGGYSVFAGVGERTREGNDLWLEMQHTHIGTSEARVLDNTCLVFG
                     QMNEPPGARLRVALTALTMAEALCEQGGGETLLFIDNIFRFSQAGSEVSALLGRIPSA
                     VGYQPTLASEMGQLQERITSTASGAITSVQAVYVPADDLTDPAPATTFTHLDSSVVLS
                     RKITEKGIYPAVDPLESSSRILDPNIVGQEHYDTAQRVLEFLQRYNSLQDIIAILGID
                     ELSREDRQIVSRARRLERFFSQPFIVSVQFTGLEGKYVSVADTVRSCREICEGRWDHL
                     PEQAFMYIGAVEEAQEKAQKK"
     gene            3835..4284
                     /gene="atpC"
                     /locus_tag="PLE88_05315"
     CDS             3835..4284
                     /gene="atpC"
                     /locus_tag="PLE88_05315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOM75920.1"
                     /translation="MVGLSQGKFRVVLLTPKAKLLDVRAGSVVVPAHDGQLGILRNHC
                     PLLAALGFGIMQVHEMADRPDAYFIIEGGFVRVSENHVTVLAYDVTTFEGKDKKEVER
                     MVSHAQSVIAGQEYIRSQQQAIDQRKARFIVQMAQMASVQSAGAESQ"
     gene            4441..4737
                     /locus_tag="PLE88_05320"
     CDS             4441..4737
                     /locus_tag="PLE88_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75921.1"
                     /translation="MMAEEGKKTENAAVNPDVAAFIRSLDEPHRMLIILKAQLYGGRW
                     EPMLDDLQNRLKGKPYIFKLSNRIKEDIERINRLQAFEAQQGIDLADYVKLESL"
     gene            4763..5881
                     /locus_tag="PLE88_05325"
     CDS             4763..5881
                     /locus_tag="PLE88_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM75922.1"
                     /translation="MKKYSLMAAVWVIGMSVHAAGFKNSPQYVLPDTAPLVPYELAAQ
                     VGWEVNWQMNLPVKTNEQIARLGVAGENLFVMTDSNVMFCIDRAQGRLRFTTPLSARN
                     LPVQPPLYYDEKFWFIVGTELLVFDPAVGDFISRKKIERINGAGANSLALNDTHLFIA
                     GSDNRIHAVNRDGYWQEFMASADNNAPIVSVLAAGSSAVFATQTGNIVGMRADGPQKL
                     WQFDATGPIDVPLVRDGAYIYAGSRDCKLYKIDMATGRLAWTAPYPSGAPIQKPFTVG
                     RKIVYLYNALNGLAGVSKETGKAVWTAPAGVDVVCETPDKGFIFAQPGVLKVVDNNTG
                     RDLYSVNFAQVQRWAVNMTDAVIYAGDVQGRLMSITVR"
     gene            5921..7531
                     /gene="purH"
                     /locus_tag="PLE88_05330"
     CDS             5921..7531
                     /gene="purH"
                     /locus_tag="PLE88_05330"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOM75923.1"
                     /translation="MDVRIRRALISVSDKTGVVEFAKGLQAMGVDIISTGGTAKTLQQ
                     AGLNVVSVESVTGFPEMMDGRVKTLHPKIHGGLLAVRDNAAHAAAMKTHHIEPIDLVC
                     VNLYPFEKTVAKAGCTLEEAIENIDIGGPSMVRSAAKNHRFVTIVTSPAQYDAVLDQM
                     KQNNGATRIELRQELARAAFALTASYDAAIAKYLDANAGELFPDRLSLAWVKQQELRY
                     GENPHQRGAFYRRTDSTEVSVGRAELLDGGTPISFNNLMDANAAFELCKEFDEPAAVV
                     VKHMNPCGCAVDEDICKAYEKAYLGDVVSAFGGIVAVNRPVDRELAAVIMESYARFGK
                     ARGAAGFFAEVIIAPSYEPDAVQTIRTLKEWGQRVRLMQTGSIDRSKTDAREYDIRCI
                     VGGLLLQQRDLAGWEPDALTFPTRLKPTKEQMEDLRIAWLTAKHVKSNTITLVKDKML
                     VGVGAGQMNRVESGLIAFKHAGCLSKGAAMGSDAFFPFPDNVENAAKAGVACIIQPGG
                     SKKDDEVIAAADRLGIAMVFAQKRHFKH"
     gene            7554..8528
                     /gene="rfaD"
                     /locus_tag="PLE88_05335"
     CDS             7554..8528
                     /gene="rfaD"
                     /locus_tag="PLE88_05335"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOM75924.1"
                     /translation="MILVTGGAGFIGSAIVAELNARGVDDIFIVDILGKDQRWKNLRH
                     LRYADYMEADDFSQMVSDSELEMDIDAVIHMGACSDTTETDCSYLVKNNYEFSKLLAG
                     WALDLEARFIYASSAATYGDGSQGFCDDEDLLETLRPLNMYGYSKQMFDLWCRRQGLL
                     DKFVGLKYFNVFGPNEYHKAEMRSFVIKAFEQINQTGSVKLFKSHRPDFPDGGQLRDF
                     IYIKDAVDMTLFFLEHPDTAGIFNIGTGKARNWNDLATAVFNAMGRPVSIEYIPMPEM
                     LRDKYQYFTQADISKLRNAGYDNDTTPLEEAVSDYVKNYLMKGEYLGA"
     gene            9077..10300
                     /gene="nusA"
                     /locus_tag="PLE88_05340"
     CDS             9077..10300
                     /gene="nusA"
                     /locus_tag="PLE88_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOM75925.1"
                     /translation="MNQELIRIVENIAKDKNIDKESLFQDLESAMVSAARKHFGIQPN
                     SGVEITVHIDRTSGTIRAFKDGVEIDIRQLGRIPAQTAKQVMIQKIKADERDSIFAQY
                     AQRKGEIVNGTVARFEGGALIVNIESRVEAIMPKSEQITGESHHLGERVRALILDVRE
                     SSSQVKIILSRTHPDFIRRLFDLEVPEVAEGIIEIKSLAREAGYRTKIAVDTTDDKVD
                     PVGACVGVRGSRIRNIVDELGGEKIDIVRWSDSSQVLIANALMPAKVSEIAICFELGR
                     ATVVVAEDQLSLAIGKHGQNVRLAARLTGWDIDILTPSEYNREVDRIAACMKDIAGLD
                     ENFVDKLLALGIISVLDLEEVGTGPLVEELQVEPALAERIIAAAEEAAKKAAAENTLT
                     QAEGLLRQEKNKSKK"
     gene            10351..13014
                     /gene="infB"
                     /locus_tag="PLE88_05345"
     CDS             10351..13014
                     /gene="infB"
                     /locus_tag="PLE88_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOM75926.1"
                     /translation="MAKASTRVHLLAKELNVPSKAIIEKCQAEGLDQITNHMSVISVG
                     LAATIREWFSEGVHATTVEQAKPVDLEKVRIKRKKVVSPQEEAAPPVEAPQEIPSSVP
                     EEVPVPQPAMPVYQEPEPMPAGLAEPASPLPETPLPQPVVEEPAAAVAAAVEPQTPPP
                     APQKPDIILPAGPMLEKPTPAVLTGPTVIRVEKVEDRYPKRHKGPRPPREKPAAESLL
                     IPADLPVKGPKAGKAKTYGRKKDKSDIDLEPGFGRGDLKRLRARDLEERQARLAAARG
                     ETVRAKPSRRIESKTAEEAAVHIERPAKAVVTEPILVKDLSNALAVKVSDIIAKLLLQ
                     GVMVTANQAISAEAAELVALEYGTELVIERKKNVLEEIEEKFNSRPRLNLQKRPPIVT
                     MLGHVDHGKTSLLDRIRKTSVAAGEAGGITQHIGAYQVELNGKKVTFLDTPGHEAFTS
                     LRARGANMTDIVVLVVAADDGVMPQTVEAIHHAKAAGVSIIVALNKIDIPGVDVNRVY
                     GQLASHDLTPTVWGGTTELVQTSAVTGAGIEELIELLDYQAELNDYRADPDVPAQGWI
                     VEAKMSQTKGPVATVLVKEGTLQKGSIVLAGGACGRIRTMTDSRGRSVKTAPPSTPVE
                     ITGLDSVPAAGDKFFCLEDLNQAKAAAEQIQHITREEGLAKRSLITLDNLFAHIEAGR
                     VKELNLIVKADVQGSVDVLIKYLTELSTDEVKVKVIHAAVGGINEGDVVLAEASSAII
                     IGFNVVPDERVRQIAESKKVDIRLYNIIYRITEDLKAAMAGLLEPEYKEQFLGRLVVR
                     DIFKVSSIGTIAGCYVSSGMVSKNAKLKLIRGGIVVKDNCSIESLKHFKNDVREVKAG
                     LECGIKVAGYDDIKIGDEFEAYEIIEVARTL"
     gene            13041..13454
                     /gene="rbfA"
                     /locus_tag="PLE88_05350"
     CDS             13041..13454
                     /gene="rbfA"
                     /locus_tag="PLE88_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOM75927.1"
                     /translation="MPSRRQQKIARVIRDSVSRTIFCRLSDPRITGLISVTEVDVSPD
                     MKNATVYLSIMAADERASSISYEAICHAIGPIQMQLGHDLIGRYCPRLRFARDEKMKK
                     TLQTLQLIEQAEQEYTAHPLQKDLPDTQDIHDEEW"
     gene            13467..14171
                     /locus_tag="PLE88_05355"
     CDS             13467..14171
                     /locus_tag="PLE88_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75928.1"
                     /translation="MKDSKLYSPKLTKLLRSLKRQAGSCSVPRYADPIEAVIYGLICE
                     QFSETAAERIYKRMKSHFVDLNDLRVSRVEEILDVFRDASPAAEHCARAITQVLNAIF
                     DKYDRIALDMLGPDGKRQAHKELSEIKGITPFAVSYCFLTALEGHAVPLNAAMVEYLR
                     KNEYVHPEASESEIAGFLERQIPANDAYTFYMLLRCLIEQGALEKPKSSEKAAASAKT
                     AKKKTAAVSKKTAKKK"
     gene            14191..15069
                     /gene="hisG"
                     /locus_tag="PLE88_05360"
     CDS             14191..15069
                     /gene="hisG"
                     /locus_tag="PLE88_05360"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOM75929.1"
                     /translation="MNAEQVLKLGIPKGSLQNATFELFERAGFNITGADRSYFPRIDD
                     PQIQLILLRAQEMSRYVEDGVLDAGFTGYDWIRENESDVYEVCELQYSKATSNPARWV
                     LAVPNESPVEKAEDLEGGIIATELVNATRRFFEQKKVNVKVEFSWGATEVKARLVSGI
                     VELTETGSSLKANNLKIIDTIMISTTRFIANKQAWADGFKREKIESIAILLNAAIDAR
                     TRVGLKMNVKKKDMPAIVKLLPAEKSPTISPLADSEYAALEIIVEEKVERELIPQLKR
                     AGASGIFTYPLNKVIH"
     gene            15102..15851
                     /locus_tag="PLE88_05365"
     CDS             15102..15851
                     /locus_tag="PLE88_05365"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM75930.1"
                     /translation="MSGHSHWAGIKHKKAVVDAKRGKLWSKIARMIIVAAKAGGGDPA
                     ANLSLRYAIEKAKAANMPKDTIEKAIKKGTGELGGVEYVEVLYEGYAPGGVAIMVEGL
                     TDNRNRTAPEIKKIFERRGGSLGVSGCVRRMFKKKGLITVKTDAVGEDELMELALSAG
                     ADDMQNTGQVYEITCAPDVFDALKSALEQKQIPLQSAELSMIPDTTVPITDVETAQKI
                     LNMMEEFEDHDDVQEVYANFDIPDEILAKIQ"
     gene            15937..18483
                     /locus_tag="PLE88_05370"
     CDS             15937..18483
                     /locus_tag="PLE88_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOM75931.1"
                     /translation="MRRVSFLSGLKLLFSFLFFAASAGAFAGGTQADYQRAAELRSRL
                     AGKVYRDQIEPNWFADGSMFWYRRQTGPNTHEFVTVDLNNPQRQPAFDHQRLAAALTE
                     QGVKDAAADRLALERLEFNLTDNTLRFQTDGKIWQCRLDTYALQIVSEAPEDSLTPMR
                     PEQASAASTRTGSQTYIIFLNQTEGEVSLFWLDSEGQRQPYGTLAAGQQRRQNTYEGH
                     VWLIVGSDGSPIAVYQAQGRPGRAVIGMEPAFRRGRRTDGTAQRPQRIRPPRAVSPDG
                     NLEAFIKDYNVWLRRLDSDQEVQLSRDGTADNFYMDRFYWSADSAKFVAVRRKPAQEH
                     KVYFVESSPRDQLQPKLHSIDYLKPGDQVDVDKPCLFNAQNEKQIPVSDDLFANPWSV
                     SDIRWMPDSSRFTFLYNQRGHQVLRIVAVDAASGTAEPLIDEKSDTFICYSSKYFCRF
                     LDESNEIIWMSERDGWNHLYLYDANSGQVKNQITKGPWVVRDVIEVDTQKRQIWLRAG
                     GIYPQQDPYYIHYVRVNFDGSGLTILTEGDGTHSVKFSPDKRYLIDTWSRVNQPPVHE
                     LRRSEDGKLLCELDRADISDLLKAGWQMPEPFVAKGRDGQTDIYGIIIRPTVFDPRLS
                     YPVIENIYAGPQDSFVPKAFSPFLRMMEMAELGFILVQIDGMGTSNRSKAFHDVCWKN
                     LSDAGFPDRILWIKAAAQKYPYMNISRVGIYGGSAGGQNAAAAVMKHGDFYKAAVADC
                     GCHDNRMDKIWWNEQWMGWPVGPEYEANSNVTLAKNMQGKLMLIVGEMDQNVDPASTM
                     QVVNALIKADKDFELLVVPGAGHGAGEGRYPSRRRADFFVRHLLGVEPRS"
     gene            18485..18817
                     /locus_tag="PLE88_05375"
     CDS             18485..18817
                     /locus_tag="PLE88_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOM75932.1"
                     /translation="MPCLVFGLPLPAGLDTISPMEPIAVQVIFRGRVQGVGFRYTARR
                     IASQHNLTGFVRNCPDGSVEALLQGQASAVDACIEDIQDSFSGYIRDIQRIPQPVNPR
                     WDDFRITY"
     gene            19139..20140
                     /locus_tag="PLE88_05380"
     CDS             19139..20140
                     /locus_tag="PLE88_05380"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOM75933.1"
                     /translation="MAASEEFKRAVELIENARSVLLTTHIRPDGDACGCIRALINILR
                     QQKKTAKPLFLSPLPSWYAALFNPRPAVLGNDLQPRQLDDFCADAELVMIVDTDSRVQ
                     LPGLADWLARCGKKVLVIDHHITSDRLGTVTITDTAAAAAGEIVFDLIKFAGWPLEGA
                     VAESIFIALASDTGWFKYGNTDSRIFRTAAELIEAGAEPNRIYQLLYQSFSPARLHLM
                     IRMLEHLQLHADGRIATQYILRSDFDQTGASGPDTENLIDECQRIQSVEAAALLVELA
                     DGGFRCSLRSKGKVDVRKIAQKYGGGGHTLAAGVNLKGPLDGALNLIVSEIKSQLTA"
     gene            complement(20451..20524)
                     /locus_tag="PLE88_05385"
     tRNA            complement(20451..20524)
                     /locus_tag="PLE88_05385"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Val,seq:cac)
     gene            20881..21159
                     /locus_tag="PLE88_05390"
     CDS             20881..21159
                     /locus_tag="PLE88_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75934.1"
                     /translation="MHGDEMDQAHFHEKLCELLNDIDGVTAPEQDKVIRLAKKTKEGH
                     ELLQKKLINLQQSLDSLRLGIKYLIFDLEATRRENNELRKRLEQEPPH"
     gene            21376..22089
                     /locus_tag="PLE88_05395"
     CDS             21376..22089
                     /locus_tag="PLE88_05395"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOM75935.1"
                     /translation="MKEWTRDNTSEFAVMTRQQVREFDAWAIGQMGIPGIVLMENAAR
                     EAAEVILRLWPKERVCIFCGGGNNGGDGFAIARHLYNHGISVQLAVCADPARIKGDAR
                     INYDICRALKLPMEVLDILSADMQQGVQKAAESYGLLVDALLGTGMQGELADSMIRLI
                     SVLNACGRPIAAVDIPSGLDCDTGLPLPVCIEAAATITFAAIKKGFTVCPQSRQATGR
                     VFVADIGIVPASIRATDSY"
     gene            complement(22067..23512)
                     /locus_tag="PLE88_05400"
     CDS             complement(22067..23512)
                     /locus_tag="PLE88_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75936.1"
                     /translation="MAVRQSSSGNTMMYALITFVALFIIAAVFAVVYYIKSEEYRTQW
                     EQAKEDLNQIANPSEKAALVRIVGKPEQGKTYLGTMQKVVDDLYAFILGQQPSADIPA
                     TVKFNEISMKIDALNKNVLGQDLNPAVGLNGVALLKTIEDLKQKLDGARQQMANLQSI
                     NDTLQSDLADAAAKSELDRQKFIAELDQFQTEMNVIRNRFDSLQKSMQDSVIEQVQTF
                     QNKLEEEQAKLRQKQLDLQETEKKLGQTDMLLKNAMSKLEAIKPKPDREVQAFQPDAQ
                     IIRVDLQNGIVYLDAGINDHVYRGLTFAIYDRNKPVSEEGQNKAEIEVFQVSEQISAA
                     RIIKSDKKNPVVKEDLVVNLIWDRKGTNQFVVAGEFDFNNDGRIDADGAQRIRELIER
                     WGGRVVEDITVDTDFLIIGSAPAALPRPTQDDLDIDPLAQQRYELSMQKVQAYDGLLK
                     KANNLGIPVFNQKRFMYLIGYDTLLNKNPSL"
     gene            23690..23926
                     /locus_tag="PLE88_05405"
     CDS             23690..23926
                     /locus_tag="PLE88_05405"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOM75937.1"
                     /translation="MQQVGPEQFGKWLAVMGLGLTAAGLFMLMLAKIGLFRLPGDLRF
                     EGENWKIFFPLTSCLVISVIVTVVFWLVQYFVRK"
     gene            23934..24719
                     /locus_tag="PLE88_05410"
     CDS             23934..24719
                     /locus_tag="PLE88_05410"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOM75938.1"
                     /translation="MTKKMQKIRKEDASATIGLVRLQRILASAGIDSRRKCEELIREG
                     AVTVNGRIIQELPAFADPQHDDIRVNGQRIRKPQKVYFLLNKPKGVICTNMDPQHRKR
                     AIDLIDCSERIFCVGRLDADTTGAIILTNDAELTNKLTHPRYELPKTYQVVVRGRMEG
                     EDVEKLKKGVWLSDGKTGAASVKILRKTNLETMLQITIRQGLNRQIRRTLAALGYKVK
                     SLKRTQIGDIVLKDIPVGGYKRLTAAQIAYLKRATESAIDRQA"
     gene            25189..25262
                     /locus_tag="PLE88_05415"
     tRNA            25189..25262
                     /locus_tag="PLE88_05415"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25223..25225,aa:Ile,seq:gat)
     gene            25378..25450
                     /locus_tag="PLE88_05420"
     tRNA            25378..25450
                     /locus_tag="PLE88_05420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25411..25413,aa:Ala,seq:tgc)
     gene            25712..>25983
                     /locus_tag="PLE88_05425"
     rRNA            25712..>25983
                     /locus_tag="PLE88_05425"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         6380 a         6285 c         7256 g         6062 t
ORIGIN      
        1 agatgctcca gttcgacggc ggggtggtcg gcgaggcatt caatctggaa gaagattccg
       61 tcggggccat tatctacggc gactacacca aagtcaaaga aggcgcatcg gtccgcgcca
      121 ccggcaaagt gctttctgta ccggtcggcg aggcgctgct ggggcgtgtg gttaacgctc
      181 tttgtgagcc cattgacggc aaaggccgca ttgcggccga gtcccggcgg ctggtcgaat
      241 ttcctgcccc cggcattgca gaccgtcagc ctgtcaagga gccgctggct accggcatca
      301 agagtgtgga tgctatgatt cccatcggcc gcggccagcg cgagctgatt atcggcgacc
      361 gcaaaaccgg caagacggcc attgcgttag acaccatcat caaccagaaa ggcaaaggcg
      421 tcgtctgcat ttatgtggcc attggccaga aggaatcgac ggtggccgaa gtggtcgcca
      481 ccctcgcccg ccacggcgct atggagcata ccattgtggt gtcggcctcg gcttcggaaa
      541 gtgcggcgat gcagtatatc gccccctatg ccggcacggc catcggggaa tattttatgt
      601 atgaaaagca cggtcatgcc ctgtgcgttt atgatgactt gagcaagcat gctatcgctt
      661 atcgcgagct gagtctgctg cttcgccgtc ccccgggacg cgaggcctat ccgggcgata
      721 ttttttacct gcacagccgg ctgctggagc gggcctgcaa gctgtccgat gcaaaaggcg
      781 ccggttctct gacagcgctg cccatcattg agacgctcga aggacaggtc tctgcctata
      841 tacccaccaa tattatttcc atcaccgatg ggcagattta cctgcagcgc tcgctgtttt
      901 tagccggtgt tcgtccggcc attgatgtgg gcatcagcgt gtcgcgtgtc ggcggggcgg
      961 cccagcccaa ggcgatgaaa aaagtggccc ccaagctgcg gctggatctg gcggccttcc
     1021 gcgagctgca ggactttgcc cgtttgggta cggaactgga cgaagcggca cagaaacagc
     1081 tggaacgcgg ctaccgaatg gtggaagtgc tcaagcagaa gcagtttgcc ccgatgagcg
     1141 tggctcagga ggtgctcagt attctggccg gttccagcgg agcgctggat gatatccccg
     1201 tgacgcgggt caaccatttt atggatgaat tgcttagctg gatgcagatt gaagctccgg
     1261 agtatctgga gtccattatg cagaagggcg acattgatga tgccctgatg accggtctga
     1321 ccgaggccat cgaggcctac aaaacgattt atcggttctc atttggagca taaagaacag
     1381 gcatcgaggt gcagttggac agcggcttgt cggcgggatt ttcagttttt tcgccggcac
     1441 agtggaaaaa cgatgcgagc cctgaatagc tggaaattga acttgttata gatggcaaat
     1501 acccgaaaaa tactgcagcg ccgcaatgcg gccgccaata tcagcaaggt gaccggtacg
     1561 atggaaacca tcagtgccgt acgctaccgc cagtattatc accagtggtc ggagggtctg
     1621 gctttttatg attcgctggc ccagctggcc tatctgatgg ttacctcgca gagcaaaatc
     1681 cagcatccgc tgctggaaga gccgaaaatc aaatccaaaa cgaatgcatt gattgtcatc
     1741 ggctcagacc ggggcctgtg cggggcctac aacaacgaaa tctatcgtca gattgatacc
     1801 catatccgca tggccaaacg gttcggacgc cagctgaaca tttatgccaa gggcaagaag
     1861 gtgctggcgt atctggagca tctgaaaata actcccgcag gaatctatga cgaattttcc
     1921 gaagtcccga cagccgagca ggcaaacaaa atcggtgatt ttttcattga agcctttttg
     1981 aaaggaaaaa tcgatcggct cggcattgtc tataaccggt tcttttcgcc ggccagccag
     2041 aaagcccaga cactgacaat tctgccggtg gtcgatttga ttgatgacct gacaacgcgg
     2101 gctacggtta tctggccgtg ggaactcaat tttgaggatt ttgaattaag tccctctccg
     2161 gaggatattt ttgagacgct ttcaaaaatg atcatccgca cttctattgc cggatgtttt
     2221 ttggaagcag ccgtcagcga gcatttgtcc cgggtggttt gcatgcgcaa tgcctcggac
     2281 aatgccgacg agatgattcg ggaattaacc aaagattata accgggcacg gcagggacag
     2341 attacggtgg aactgcttga tattgtcagc ggtgtagaag ccgcaaaaag ataaattttt
     2401 taatgaagct caattatgaa tcgcggacgc attacacaag ttataggaag tacatttgaa
     2461 gccgaatttg ccgctcaggt tccggcggtt tacaatgccc ttgaagtaaa aggcgagttt
     2521 gaaggcggcc ggattgattt ggttggcgag gtgcagcagc atctcggcgg cggacgggtg
     2581 cgggcggtgg ctctgggcag cacagccggt cttcggcgcg gaatggaagt aatcgacacc
     2641 ggaaatcccc ttatggtgcc ggttggcaaa gaaaccttgg gacgggtatt taaccttctg
     2701 ggcagacctg ttgacggcgg acctgatatc agcgtcaagg aaaaacgccc gattcatcga
     2761 aaaccgccca aatttactgc cttgacggct aaatccgaaa tgtttgagac cggcatcaaa
     2821 gttatagacc ttctttgtcc gtttgttaaa ggcggtaaga ccggcctgtt tggcggggca
     2881 ggtgtcggca agacggtgct cattcaggaa ctgattgccc gtattgcgac ccagcacggg
     2941 ggctattctg tctttgccgg tgtgggcgaa cgaacccgtg agggcaatga cctctggctg
     3001 gaaatgcagc acacgcacat cggcacatcg gaagcgcggg tgctggacaa cacctgtctt
     3061 gtttttggtc agatgaatga gccgccgggg gctcgtctgc gtgttgcgct gaccgccttg
     3121 accatggccg aagccctgtg cgaacaaggc ggcggcgaga cgctgctgtt tatcgacaat
     3181 attttccgct tttcgcaggc cggttctgaa gtatccgctc tgctggggcg cattcccagc
     3241 gctgtcggat accagcccac gctggccagc gaaatgggac agcttcagga acgcatcacg
     3301 tcgactgcat caggggctat cacatcggtt caagccgttt atgtgccggc tgatgaccta
     3361 actgacccgg cccctgctac gacatttacg catttggatt cgtcggttgt attgtctcgt
     3421 aaaatcacgg aaaaaggcat ttatccggct gtggatccgc tggaaagcag ttcccgaatt
     3481 ctggacccga acattgtggg acaggagcat tacgatacag cccagcgggt gctggagttt
     3541 ctgcagcgct ataacagcct gcaggatatt attgcgattc tcggcattga tgagctcagc
     3601 cgcgaagacc gtcagattgt ttcccgcgca cgccgtctgg agcggttttt ctcacagccg
     3661 tttattgttt cggttcagtt tacgggtctt gaaggcaagt atgtttccgt tgctgatacg
     3721 gtgcgaagct gccgggaaat ctgtgaaggc cgatgggatc acctgccgga acaggccttt
     3781 atgtatatcg gagcggttga agaggcacaa gaaaaggcgc aaaagaagta acatatggtc
     3841 ggattgtcgc aaggaaaatt tcgcgttgtt ctgctgacgc caaaggcaaa attgctggat
     3901 gtccgagccg gttctgttgt tgttccggcc cacgacggac agctgggcat cctccgcaat
     3961 cattgtccgt tgctggcggc gctgggattt ggcattatgc aggttcatga aatggcggat
     4021 cgtcctgatg cctactttat cattgagggg ggctttgtcc gtgtcagcga aaaccatgtg
     4081 acagtgctgg cctatgatgt gacgacattc gaaggaaaag acaaaaaaga agttgaacgg
     4141 atggtttctc atgcgcaaag cgtcatcgcc gggcaggaat acatccgctc ccagcagcag
     4201 gccattgacc agcgaaaagc gcggtttatt gttcagatgg cgcagatggc ttctgtccag
     4261 tctgctgggg cagagagcca ataggaagca gtgttttttg cctttatccg ggcggactgg
     4321 acagtcaagc ccggctgcgg ttgcgaaagt gtcatatttt tactgggtcc agaaagaaag
     4381 catttggcag cagttttttt atgggctaca ataacacggt aatcttttgt ggataaggca
     4441 atgatggcag aggaaggcaa aaaaaccgag aatgcagctg taaatccgga tgtggctgcg
     4501 tttatccggt cccttgatga gccgcatcga atgttgatta tcctcaaagc gcagctctac
     4561 ggcggccgct gggagccgat gctggatgac ctgcagaacc ggttgaaagg aaaaccctat
     4621 atctttaagt tgtccaaccg catcaaagaa gacattgagc gaatcaatcg gctgcaggcc
     4681 ttcgaagccc agcaggggat tgatttggcc gattatgtaa aactggaatc attgtagcag
     4741 cggagaataa ggattgatga atatgaaaaa atacagcttg atggcagccg tatgggtcat
     4801 tgggatgtcg gtccatgcgg ccggttttaa gaactccccc caatatgtcc ttccggatac
     4861 cgctcctctg gtgccctatg agctggcagc gcaggttggc tgggaggtca attggcagat
     4921 gaatctgcct gtcaaaacca atgagcagat agcccgtttg ggcgttgccg gcgagaatct
     4981 gtttgtcatg acggattcga atgtgatgtt ctgcatcgac cgtgcacaag gccggctgcg
     5041 gttcactacc cccctaagcg ctcgcaatct gcctgtgcag ccgccgctgt attacgatga
     5101 aaaattctgg tttattgtcg gtacagaact gctggtattt gatccggcag tgggcgattt
     5161 cataagccgc aaaaagatag agcgcatcaa tggggccgga gccaacagtc tggctctcaa
     5221 tgatacgcat cttttcattg ccggttcgga taaccgcatt cacgctgtca accgtgacgg
     5281 ctactggcag gagttcatgg cttcggcgga taacaatgcc cctattgtgt ctgtgctggc
     5341 cgccggcagc agcgcagttt ttgccaccca gaccggcaat attgtgggaa tgagagccga
     5401 tgggcctcaa aaactgtggc agtttgacgc aacaggtccg attgatgttc ctctggtgcg
     5461 ggacggggcc tatatttatg ccggcagccg cgactgcaag ctgtataaaa ttgatatggc
     5521 taccggccgt ctggcatgga cagcgcccta tccttcgggt gcccccatcc agaaaccctt
     5581 cactgttgga cgaaagattg tctatctcta caatgcatta aacggcttgg ccggcgtcag
     5641 caaagaaacc ggcaaggccg tctggaccgc accggcaggc gttgacgtgg tttgtgaaac
     5701 tccggacaaa ggttttattt ttgcccagcc cggtgtgctc aaagtcgtgg ataataatac
     5761 aggccgggat ttgtattcgg tcaatttcgc gcaggtccag cgatgggccg tgaatatgac
     5821 cgatgcggtt atatatgcag gcgatgttca aggacggctg atgagcatca ccgtccggta
     5881 agcagtacaa gcgaattgat tggaaaggga ttgcgaaaca atggatgtgc ggattcggcg
     5941 ggcgttaatc agtgtctcgg ataaaaccgg tgttgtggaa tttgccaagg ggcttcaggc
     6001 catgggcgtc gacattatca gcaccggcgg aacagccaag acgcttcagc aggcagggct
     6061 caatgtggtc agtgtggaat cggttacggg atttcccgaa atgatggacg ggcgagtcaa
     6121 aacgctgcat ccgaagattc acggggggct actggcggtt cgcgacaatg cggctcatgc
     6181 agcggcaatg aagacccatc acattgagcc gatagactta gtctgtgtca atttgtatcc
     6241 cttcgagaag actgttgcta aagccggctg cacactggaa gaagcgatcg aaaatattga
     6301 catcggcggc cccagcatgg tgcgctcggc tgcaaaaaat catcggtttg taaccattgt
     6361 aaccagtccg gcccaatacg atgctgtttt ggaccaaatg aagcagaaca acggtgcaac
     6421 ccgcatcgag ctgcggcagg aactggccag agcggccttt gccctgacgg cttcgtatga
     6481 tgccgccatc gctaaatacc tcgatgccaa tgcaggcgag ctgtttccgg accgcctgtc
     6541 gctggcatgg gtcaaacagc aggagctgcg ctacggagag aatccccatc agcggggggc
     6601 cttttaccgc cggacggaca gcaccgaggt cagcgtcggt agagccgagc ttctcgacgg
     6661 cggaacgccg ataagcttta ataacctgat ggatgccaat gcggcatttg agctgtgtaa
     6721 agaatttgat gagccggccg ctgtggtcgt caagcatatg aatccctgcg gctgtgcagt
     6781 tgatgaggat atctgcaaag cctacgaaaa ggcctatctg ggcgatgtcg tcagtgcctt
     6841 tggggggatt gtggcggtaa accgccctgt ggacagggaa ctggccgccg ttattatgga
     6901 atcttacgcc cgcttcggca aggcgcgggg cgccgccggt ttttttgccg aagtgatcat
     6961 tgccccgtcc tatgagcccg atgccgtcca gaccattcgt actctcaaag agtggggcca
     7021 gcgggtgcgc ctgatgcaga ccggatccat cgaccgttcc aaaacggacg cccgcgaata
     7081 cgatatccgc tgtatagtcg gcggtttgct actgcagcag agggatttgg ccggctggga
     7141 accggatgcc ctgacttttc cgacacggct gaagcccaca aaagagcaga tggaagacct
     7201 gcggattgct tggctgacag ccaaacatgt caagagcaat acgattacgc tggtcaagga
     7261 caaaatgctg gtcggagtgg gtgccggcca gatgaatcgt gttgaatccg gtctgattgc
     7321 cttcaagcac gccggctgcc tctccaaagg tgccgcaatg ggatcggatg catttttccc
     7381 cttcccggac aatgtggaaa acgccgccaa agcgggagtg gcctgcatta ttcagccggg
     7441 cggctcgaaa aaggacgatg aggtcattgc tgctgccgat aggctgggaa tcgcaatggt
     7501 ctttgcccaa aagcggcatt tcaaacatta acactattac ggagggcagt gtaatgatac
     7561 tcgttaccgg cggggcaggt ttcatcggtt cggccattgt cgccgaactg aacgcacgcg
     7621 gcgtggatga tattttcatt gttgatatct taggcaaaga ccagcggtgg aaaaacctcc
     7681 ggcatctccg ctatgccgat tatatggaag cggatgattt ttcccaaatg gtttccgatt
     7741 ccgagctgga aatggatatc gatgcagtca ttcatatggg ggcctgttcc gataccaccg
     7801 aaacagactg ttcctatctg gtaaagaaca attatgaatt ttcaaagctt ctggccggct
     7861 gggcgctgga ccttgaagcc cgcttcatct atgcttccag tgcggccact tacggcgacg
     7921 gctctcaggg cttttgcgat gatgaagatc ttctggaaac actgcgaccc ctgaatatgt
     7981 acggctacag caaacaaatg tttgacctgt ggtgccgcag acagggcttg ctggacaagt
     8041 tcgtcgggct taaatacttc aatgtctttg gccccaatga atatcacaag gccgagatgc
     8101 gcagttttgt cattaaagcc ttcgagcaga taaaccaaac cggaagcgtc aaactcttta
     8161 aatcccaccg tcccgatttc cccgacggcg ggcagcttcg ggatttcatt tatatcaagg
     8221 acgctgtcga tatgacgcta tttttcctgg aacatcccga tacagccggc attttcaata
     8281 ttggcacagg caaagcccga aactggaacg atttggcaac tgctgttttt aatgccatgg
     8341 gcagacctgt gtccattgaa tatattccaa tgccggaaat gcttcgggat aagtaccaat
     8401 atttcactca ggccgacatc tccaagctcc gcaatgccgg ttatgataat gatacaacac
     8461 ccttggagga ggcagtgagc gattacgtca agaattatct gatgaaaggc gagtatctgg
     8521 gggcttaatt gctggtatct gccggctgtt attcacactt ttttttctgc ggctgaacag
     8581 gccaattatt taatcctgtt atttattctt tgagttggtt tttggttttg attgtttaga
     8641 aatctttaaa ttcaggtgta gtatatcgcc ttcttgccgt tttgattgcc ggcatgagaa
     8701 atcttgcgga ttgtcgatta taaaccttga caggctggcg gtttctcagg taatatgctt
     8761 ttttgcggca ggaacgcaat gatgaaagga tagcaacaat gttgcagata ggaaaagtaa
     8821 gtcgtgcgta cgcatagggt tacagtgtac aagacggttt ctcgtacgct gtgcggccgg
     8881 ctgcttttga atcatagacg ctcttcaggg actttgcaga attgtttttt ctgggagggt
     8941 gcaatggccg aagggtagaa ccaatgtgtt caacccggct cggttgaaat gtaccgggcc
     9001 tgtgcaagtt ctgcatttgt cgaagtggca acctgaaggt ttccactttt tttttggcat
     9061 gaaggaaaag gacaagatga atcaggaatt gattagaatt gtagaaaaca ttgctaaaga
     9121 caagaatatt gataaagagt ctcttttcca agacttggaa tcggcaatgg tttccgctgc
     9181 ccgcaagcat ttcggcatac agccgaattc cggcgttgaa attacggtcc atattgaccg
     9241 tacgtcggga actatccgtg cattcaagga cggcgtggaa attgatattc ggcagcttgg
     9301 gcggattcct gcacagacag ccaagcaggt aatgattcaa aaaatcaaag ccgatgagcg
     9361 cgacagtatt tttgcccaat atgctcagcg taagggcgaa attgtcaatg gaacagtcgc
     9421 ccgctttgaa ggaggggcgt tgattgtcaa tatagaaagc cgggttgagg cgataatgcc
     9481 caaatccgag cagattacag gagaatccca tcacctgggc gaacgtgttc gtgccttgat
     9541 tctggatgta cgcgagtcgt caagtcaggt taagattatt ctgtctcgaa cacatccgga
     9601 ttttatccgc cggttgtttg atttggaagt acccgaggtg gccgaaggta ttattgaaat
     9661 taaatcactg gcccgagaag ccggctatcg gaccaaaatc gcagtggata caaccgatga
     9721 taaagtcgat ccggtgggcg cttgtgtcgg ggtgcggggc agccggatac gcaatattgt
     9781 tgatgaactc ggcggggaga aaatagacat tgttcgctgg agcgattctt cgcaggtatt
     9841 aattgcaaat gctctgatgc ctgccaaggt cagtgaaatt gctatctgtt ttgaattggg
     9901 ccgagcaact gttgtcgtcg cagaagacca gctcagtctg gctatcggca agcacggcca
     9961 gaatgttcgt ctggcagcgc ggctaaccgg ctgggacatc gacattctga cgcccagtga
    10021 gtacaataga gaagttgatc gcattgctgc ctgcatgaag gacattgccg gtttagatga
    10081 aaactttgtc gataaattgc tggcactggg tatcatctca gtactggatc tggaagaagt
    10141 cggcaccggt ccgctggttg aggaactgca ggtggaaccg gcgctggccg aacgcattat
    10201 tgctgctgcg gaagaagccg ccaaaaaagc agccgcggag aataccttaa cacaggccga
    10261 agggcttttg aggcaggaaa aaaataagtc aaaaaaataa aatcaggata agcaggcggc
    10321 gctgattggg attctttaca ggagacggtt ttggcgaaag caagcacacg cgtacattta
    10381 ttggccaagg aattgaatgt ccccagcaag gccattattg aaaagtgtca ggcagagggg
    10441 ttggatcaga ttaccaacca tatgtcggtc atttctgtag gattagccgc aaccattcgc
    10501 gaatggttca gtgagggagt ccacgctacc acagtcgagc aggccaaacc cgttgacctc
    10561 gagaaggtgc gaatcaagcg taaaaaagtt gtgtcccctc aggaagaagc agctccgccg
    10621 gttgaagctc cgcaggaaat accctcttct gtgccggagg aagttcctgt cccccagcct
    10681 gcgatgccgg tttatcagga gcccgagccg atgccggccg ggttggcaga accagccagc
    10741 ccgctgccgg agacgccgct tcctcagcct gttgtcgaag aacctgccgc agcggttgct
    10801 gctgctgtgg agccccaaac tccgcccccg gcgccgcaga aaccggacat tattttgccg
    10861 gccggcccca tgcttgagaa acccactccc gctgtgctga ctggtccgac ggttattcgt
    10921 gtcgaaaagg ttgaagatag atatcccaaa cggcacaaag ggccgcggcc tccccgcgaa
    10981 aagcctgctg ccgaatccct gctgattcct gcggatctgc cggtcaaggg gccgaaggcc
    11041 ggaaaagcca aaacatacgg ccggaagaaa gacaaaagcg atattgattt agaacccggt
    11101 ttcggccgcg gcgaccttaa gcgtctgcgc gcccgtgatc tagaagaacg ccaggcccgt
    11161 ttggcagctg cgcggggcga gaccgtgcgg gcgaaaccgt cgcgccgcat tgagtccaag
    11221 acagccgagg aagcggcagt ccatattgaa cggcctgcaa aagctgttgt aaccgagccg
    11281 attctggtta aagatttgtc gaatgcttta gcagtcaagg tcagcgatat catcgccaag
    11341 ctgctgctgc agggtgttat ggttacagcc aatcaggcca tctccgccga agccgcagag
    11401 ttggtggcgc tggagtatgg aaccgaactg gttattgaac gcaaaaagaa tgttctggaa
    11461 gaaatagaag agaagttcaa cagccgcccg cggctgaatc tccagaagcg tccgcccatt
    11521 gtaacgatgc tggggcatgt cgaccacggc aaaaccagtc tgctagacag aatccgcaaa
    11581 accagcgttg ccgcaggtga agccggcgga attacccagc atatcggggc ctatcaagtg
    11641 gagctgaacg gcaagaaggt cacttttctg gatacgcccg gccatgaggc atttacatca
    11701 ctgagggcac gcggggccaa tatgacagat attgttgtct tggtggtagc cgccgatgac
    11761 ggcgtcatgc cgcagactgt tgaagccatt caccatgcca aagcggccgg cgtgtctatc
    11821 attgtggcct tgaataaaat tgatattccc ggtgtggatg tgaaccgggt ttacgggcag
    11881 ttggccagcc atgatctgac gccgaccgtc tggggcggca caaccgagct tgtgcaaacc
    11941 agtgctgtta ccggggcggg catcgaggaa ctgattgaac tgctggatta tcaggcagaa
    12001 ctgaatgatt accgggctga tccggatgtg ccggcgcagg gctggattgt agaagctaaa
    12061 atgtcccaga ccaagggacc tgtggcgacg gtcttggtca aggaagggac actgcagaag
    12121 ggctccattg tgctggccgg aggggcctgc ggacgcatcc ggacgatgac ggatagccgc
    12181 ggacggagcg ttaaaacggc accgccttcg acgccggttg aaattaccgg attggacagc
    12241 gttcctgctg ccggcgataa attcttctgt ctggaagacc tgaatcaggc caaagcggcc
    12301 gccgaacaga ttcagcatat cacccgcgaa gaaggtctgg ccaaacgctc gctcattacg
    12361 ctggataatc tctttgccca tattgaagcc ggacgcgtta aggagttgaa tctgattgtc
    12421 aaagccgatg tacagggctc cgtcgatgtg ctgattaaat acctgacaga attaagcacc
    12481 gacgaggtca aagtgaaggt cattcatgcg gcagtgggcg gcatcaatga aggcgatgtc
    12541 gtgctggcag aggcctcctc ggctattatc atcggattta atgttgttcc cgatgagcgc
    12601 gtccgccaga ttgccgagag caagaaagtg gatattcgtc tgtataatat catttatcgc
    12661 attaccgaag acctcaaggc cgcaatggcc ggactgctgg aaccggaata caaagaacag
    12721 ttcctcggac ggctggtggt gcgtgatatc ttcaaggtct ccagcatcgg gaccattgcc
    12781 ggatgttatg tgtccagcgg aatggtttcc aaaaatgcca aactcaaact gattcgcggc
    12841 ggcattgttg tgaaagacaa ctgctccatc gagtctctga agcactttaa aaacgatgtc
    12901 cgggaagtca aggcgggtct ggaatgcggc atcaaggtgg caggatacga tgatattaaa
    12961 atcggcgatg aatttgaagc ctatgaaatt atagaagtcg cccggacgct gtagtccgtt
    13021 ggaacatcgg agaacaagtt atgccctcca gaagacagca gaaaattgca cgggtgatac
    13081 gggattcggt cagccggact atattctgcc ggctcagcga cccccgcatc accggattga
    13141 tcagcgtaac ggaggtggat gtttcgccgg atatgaaaaa tgcgaccgtg tacctgagca
    13201 tcatggccgc agacgagagg gcctccagca tttcgtacga agcgatttgc catgcgattg
    13261 gtccgattca aatgcagctg ggacacgact tgatagggcg gtactgtccg cgtctgcggt
    13321 ttgcacggga cgagaaaatg aaaaagaccc tgcagacgct tcagctgatt gaacaagctg
    13381 aacaggaata tacggctcat ccgcttcaga aagatttgcc ggatacgcag gatatccacg
    13441 acgaagaatg gtgagaggtg cacggtatga aagacagcaa actttacagc cctaaactaa
    13501 caaagcttct gcgttcgctc aagcgtcagg caggcagctg ttcggtgccc cgttacgccg
    13561 atccgataga ggcggttatt tacggtttga tttgcgagca attcagcgaa accgctgccg
    13621 aacgcatcta taagcggatg aaaagccatt ttgtggatct caatgacctg cgggtgtccc
    13681 gcgttgaaga gatactggat gttttccggg atgcctcgcc ggcggcggaa cattgcgcac
    13741 gggcaattac acaggttctg aatgcgattt ttgacaaata cgatcgcatt gcgctggata
    13801 tgctgggtcc ggacggaaaa cgtcaggccc ataaggaact gtctgaaata aaaggcataa
    13861 cgcctttcgc cgttagctat tgttttttga ctgcattgga ggggcatgcc gtgcccttga
    13921 atgcggctat ggtagagtac cttcgcaaaa atgaatatgt ccaccccgaa gcgtcggagt
    13981 ccgaaatcgc cggtttcctt gaacggcaga ttccggccaa cgatgcgtat actttttata
    14041 tgctgctgcg ctgtctcatc gagcaggggg ccttggaaaa gcccaagagc agcgagaaag
    14101 ccgctgcttc agccaaaacg gcgaagaaaa agactgcggc agtgtccaag aaaacagcaa
    14161 aaaagaaata gtgtgtcagg aaacaacagg atgaatgcag aacaggtgct taaattaggc
    14221 attccaaagg gaagcctgca gaatgcaaca tttgaactgt ttgaacgggc tggattcaat
    14281 attaccggcg ccgatcgcag ctattttcca cggattgacg acccgcagat tcagctgata
    14341 ctgctgcggg cgcaggagat gagccggtat gttgaagacg gtgtgctgga tgcgggattt
    14401 accggctacg attggattcg ggaaaatgaa tccgatgttt acgaagtctg tgaactgcag
    14461 tacagcaagg caacctccaa tcccgcacgc tgggtgctgg cggtgcccaa tgagtcaccg
    14521 gtcgaaaagg ccgaagacct tgaaggcggc atcattgcta cagagctggt caacgctacc
    14581 cgccggtttt ttgagcagaa aaaggtcaac gtcaaagtcg agttcagctg gggagccacc
    14641 gaggtcaagg cgaggctggt cagcggcatt gtggaactga cggaaaccgg ctcatccctg
    14701 aaggctaaca acctgaagat tattgatact atcatgatat ccaccacccg ttttattgcc
    14761 aataaacagg catgggcaga tggtttcaaa agggaaaaaa tcgaaagcat tgccattttg
    14821 ctcaatgctg ctattgatgc cagaacccgt gtggggctga agatgaatgt gaaaaaaaag
    14881 gacatgccgg ctatcgtaaa gcttctgccg gctgaaaaaa gccccacgat ttcgcccttg
    14941 gctgattcgg agtatgcagc gctggagatt attgtggaag aaaaagtgga gcgtgagctg
    15001 attcctcagt taaaaagggc cggggcttcg ggcatcttta cctatccgct caataaagtg
    15061 attcattaac tgcacagata agtttttgca aaggatgatg tatgtcagga cattcacatt
    15121 gggccggcat caagcataag aaagccgttg ttgatgccaa acgcggcaaa ctctggagca
    15181 agattgccag aatgattatt gtagcggcca aagcaggagg cggcgatcct gctgccaatc
    15241 tgtccctccg ttatgcgata gaaaaggcca aggcggccaa tatgcccaaa gatactatcg
    15301 aaaaggccat taaaaagggc accggcgaac tgggcggcgt cgagtacgtg gaagtactct
    15361 acgagggata tgcccccggg ggagtagcaa ttatggttga aggccttacc gacaaccgta
    15421 accgcacagc gccggaaatc aagaaaatct ttgaacggcg gggcggttcg ctgggggtca
    15481 gcggctgtgt ccggaggatg tttaagaaga aaggccttat taccgtcaag accgatgccg
    15541 tcggggaaga cgagctgatg gagctggcac tgtcggcagg ggcagatgat atgcagaata
    15601 ccggtcaggt ctatgagatt acctgtgccc cggatgtgtt tgatgccctc aaaagtgccc
    15661 ttgaacagaa gcagattcct ctgcagagtg ccgagctgtc aatgattccc gatacgaccg
    15721 ttcccatcac cgatgtagaa acggcccaga agattcttaa tatgatggag gaattcgaag
    15781 accacgacga tgtgcaggag gtgtatgcca acttcgatat tccggacgag attcttgcca
    15841 aaatccagta ggataactgc tgttttataa agggggatag tctctcggct ccggctgtcc
    15901 cggcaaacag ggatttattt gaaagggaaa gaaaaaatgc gcagagtatc ttttttgtct
    15961 ggtttgaagc ttcttttttc ttttctgttt tttgcagcat cagctggagc ttttgcaggg
    16021 ggaacgcagg ccgattacca gcgggcggcc gagctgcgca gccgattagc cggcaaggtt
    16081 taccgggacc agatagaacc gaactggttt gccgacggct ctatgttctg gtaccgccgg
    16141 cagaccggcc ccaatacgca tgaatttgtt acggttgatc tgaacaatcc ccagcgtcag
    16201 ccggccttcg accatcagcg gctggcggcg gcattgacag aacaaggagt caaggatgca
    16261 gcagccgacc ggctggctct ggaaagactg gagtttaatc tgacagacaa taccctgcga
    16321 ttccagaccg acggcaaaat ctggcagtgc cgtctggata cgtacgcgct tcaaattgta
    16381 tcagaagccc ccgaggattc tttgactccc atgcgtccgg agcaggcgtc ggcagccagc
    16441 acccgcacgg gcagtcaaac ctatattatc tttttgaacc agaccgaagg ggaagtttcc
    16501 ctgttctggc tggacagcga gggccagcgg cagccctatg gtactcttgc tgccggccag
    16561 cagcgccggc agaataccta tgaaggccat gtatggctga ttgtgggctc cgacggcagt
    16621 ccaatcgctg tttatcaggc ccaaggccga ccgggacggg ctgttatcgg aatggaacct
    16681 gctttccgcc gcgggcgccg aaccgatggc actgcccagc ggccccagcg catccggccg
    16741 ccgcgggctg tcagccctga tggaaatctg gaagcgttta ttaaggatta caatgtctgg
    16801 ctgcgccgtc ttgacagcga tcaggaagtc cagctcagcc gcgatggaac tgccgacaac
    16861 ttctatatgg atcgcttcta ttggtctgct gattcagcaa aatttgtggc cgtccgccga
    16921 aaaccggctc aggaacacaa ggtctatttt gttgaatcat cgccaagaga tcagcttcag
    16981 cccaaactcc attccatcga ctatctcaaa ccgggcgatc aggtggatgt ggacaagccc
    17041 tgtctgttta atgcccaaaa cgaaaagcag attcctgtca gcgatgacct gtttgccaat
    17101 ccatggagtg tttccgatat acgctggatg ccggattcaa gccgctttac gttcctctat
    17161 aatcagcggg gccatcaggt gctgcggatt gtggcggtgg atgcagcatc cggtacggct
    17221 gagccgctta ttgatgagaa aagcgataca tttatctgct attcgagcaa gtatttttgc
    17281 cgctttctgg atgaatcgaa tgaaattatc tggatgtccg agcgggacgg ctggaatcat
    17341 ctctatctgt atgatgccaa ttccggacaa gtcaaaaacc agattaccaa aggtccttgg
    17401 gtcgtcagag atgtcataga ggtcgatacc caaaaacggc aaatctggct gcgtgccggc
    17461 ggcatttatc cgcagcaaga tccttactac atccattatg ttcgcgttaa cttcgacggg
    17521 tccggcttga ctatcctcac cgaaggcgat ggaacgcatt cagtcaagtt ttcccccgac
    17581 aagcgttatt tgattgatac ctggtcgcgc gtcaaccagc cgccggtgca tgagctgcgc
    17641 cgcagcgagg acggtaaact cctgtgcgaa ctcgatcgag ccgacatatc ggacctgctc
    17701 aaggccggct ggcagatgcc cgaaccgttt gttgccaaag gccgcgatgg acaaaccgac
    17761 atttacggca tcattatccg cccaacggtt ttcgatccga gactgtcgta tccggtgatt
    17821 gagaatattt acgccgggcc gcaggattca tttgtcccca aggctttctc gcctttttta
    17881 cggatgatgg aaatggccga actgggcttt attcttgtcc agattgacgg gatggggact
    17941 tccaatcgct caaaggcctt tcatgatgtc tgctggaaaa atctctcgga tgccggattt
    18001 cccgaccgca tcctttggat caaggccgct gcgcaaaaat acccgtatat gaatatcagc
    18061 cgggtgggta tctatggcgg ctctgcaggc ggccagaatg ccgccgccgc cgtgatgaag
    18121 catggcgatt tttataaggc agcggtagcc gactgcggct gtcatgataa ccggatggac
    18181 aaaatctggt ggaatgagca atggatgggc tggccggtag gtcccgaata tgaagccaat
    18241 tccaatgtta cattggccaa aaatatgcag ggcaaactca tgctgattgt cggggaaatg
    18301 gaccagaatg tcgatccggc ttcgacaatg caggttgtca atgcccttat taaagccgac
    18361 aaggatttcg aactgctggt ggtcccgggg gcagggcatg gagcagggga aggacgctat
    18421 ccaagccggc gtcgagccga cttctttgtc cgccacctgc tgggtgttga accgcgcagc
    18481 tgaaatgcct tgtttagttt ttggcttgcc gctgccggcg ggcttggata caataagccc
    18541 tatggaaccg atagccgtac aggtcatttt cagggggcgg gtgcagggtg tgggcttccg
    18601 ctatacagca cgccgcatcg catcccagca caacctaacc ggttttgtgc ggaactgtcc
    18661 cgacggttcg gtcgaagcgc tgctgcaggg gcaggcttct gcggtggatg cctgcatcga
    18721 ggatatccaa gacagcttca gcggttatat tcgtgatatc caaaggatac cccagccggt
    18781 caatccccgc tgggatgatt tccgaataac ttattgaatt tcattttggg atcccgtcta
    18841 atacagcatt ctggaaaaat agaccagcaa gagccgctga tcgatgagga tgcgttaaaa
    18901 aggagcagca cggcctgatg caggcggcag gcccctcaag ggtgtttggc cggagggtaa
    18961 tatgctgtta aagctgcgcg gatttcgagc cgatagacag atttcggaaa cgcaatgcaa
    19021 taaaatccgc gccagaggaa aaacagactt gattggatta ttcctgccgg cggcggcttt
    19081 ctaatctgcc tgctgcagat ttataataag atagggaaca agcagaggat ggacatcgat
    19141 ggcagcatct gaagagttca aaagagccgt tgaactgatt gaaaacgccc gcagtgtttt
    19201 gctgaccacg catatccggc cggatggcga cgcctgcggc tgcatccggg cgctgataaa
    19261 cattcttcgt cagcaaaaga aaactgcaaa accgctcttt ctttcgccgc tgccttcttg
    19321 gtatgccgct ttatttaatc cgcggcctgc tgttctgggc aatgatctac agccgcggca
    19381 gctggacgat ttttgtgccg atgccgagct tgtgatgatt gtcgataccg acagccgggt
    19441 ccagcttccc ggtttggctg actggcttgc ccgctgcggc aaaaaagtcc ttgtgattga
    19501 ccatcatatt accagcgacc ggctgggtac agtaaccata accgacaccg ccgccgcagc
    19561 cgccggcgaa attgtttttg acctgattaa gtttgccggc tggccgcttg agggtgctgt
    19621 agccgaatcg atttttattg ctcttgcttc ggataccggc tggttcaagt acggcaatac
    19681 cgacagccgc atcttccgta ccgccgcgga gctgattgaa gccggcgctg aacccaacag
    19741 gatatatcag cttctttatc agtctttttc tccggcccgg ctgcatctga tgattcgtat
    19801 gctcgagcat ctgcagctcc acgccgacgg ccgcattgcc acgcagtata ttctccgcag
    19861 cgattttgac caaaccggcg cctccggccc cgacaccgaa aaccttatcg acgaatgcca
    19921 gcgtatccag tccgtcgaag ccgccgccct ccttgtcgaa ttggccgacg gcggctttcg
    19981 ctgctccctt cgcagcaagg ggaaggttga tgtccgaaaa atcgcccaaa agtacggcgg
    20041 cggcggtcat accttagccg ccggcgttaa cctcaaaggg cctctcgacg gtgccctaaa
    20101 ccttatcgtc agcgaaatca aatcccagct gacagcatag gcactgtgta ttttgtatca
    20161 atttcaccgt tatttaaaat cagaatccaa tatgtcttga agttgccgtt tcctattgac
    20221 gacttgctgc acggtttaaa tcttgataac cttgccaagc tgggaatctt cgggccgcat
    20281 atcggattgc gggaaggaat agtggataag acaattttac gcttaaaagc tctgcgacgg
    20341 tctgtatttt cttaatgatt aaaaaaatct tggcctgagg actttggcgg ggcacaaatt
    20401 taagcatttc ctaaaatcgc aagttgcgat ttcatttgaa cttacagcac tgggcgatac
    20461 aggattcgaa cctgtgacct cacgggtgtg atccgtgcgc tctaaccagc tgagctaatc
    20521 gccccgaaaa aactgattta agcatagccg cactgggctg tcaagcagtt tccctttccc
    20581 ctgcaggcat attgtcaaaa cggctgttta tacaccgtat aaaagacaaa aaaaatatca
    20641 tgaaagaccg ataaagtatt tattttttga gtaatttatg gattttttta ggattctgaa
    20701 ataaaatcag taaaatttcg gataaaaagg cagtaatttg ctataatacg cttaatggct
    20761 tcggggtgac agcaagcata tcaaaaaaat gaccgttttc agggccgaaa acgggggaca
    20821 attcagcgac ggatccatta tacgaacaca atatgtgaat cggcttcgga gaatgtagct
    20881 atgcacggtg atgaaatgga tcaggcacac ttccacgaaa aactctgcga attgctgaat
    20941 gatatcgatg gtgtaacggc accggagcag gacaaagtaa tccggctggc caaaaagacc
    21001 aaagaagggc acgaattgct gcagaaaaaa ctgatcaatc tgcagcagtc gctggattca
    21061 ctacggctag gcatcaaata tctgattttt gatttggagg ccacccgccg ggagaataat
    21121 gagcttcgca agcgtctgga gcaagaaccg ccgcattaaa agaatctgcg acagacggtt
    21181 gcaaacaaca cagcagttat gccgccggct gtggaggggt gtttgactgc tttgaatgtg
    21241 cgccgtgcgg tctatcaagc ctatcaactc tgtactttca atttctgcag aatcgtgtat
    21301 atttattgac agcggttcct ttggaaggtg cgatgttgcc gccttattgc ctcgattggt
    21361 caaaaaggaa aagcgatgaa ggaatggacc agagacaaca cgagtgaatt tgctgtaatg
    21421 acacgtcagc aagtgcggga gtttgatgct tgggctattg gccagatggg cattcccggc
    21481 attgtattga tggaaaatgc cgcacgcgag gctgccgagg tgattttacg cttatggccc
    21541 aaagagaggg tttgtatttt ttgcggcggg ggcaataacg gcggcgacgg ctttgccata
    21601 gcccggcatc tgtataatca cggcatatct gtgcagctgg cggtgtgtgc cgatccggcc
    21661 agaattaaag gagatgcccg catcaactat gatatctgcc gtgccctgaa actgccgatg
    21721 gaggtactgg acatcttgtc tgccgatatg cagcaggggg tgcaaaaagc ggccgaatcc
    21781 tacggtctgc tggtagatgc tctgctggga acggggatgc agggcgaact ggcagatagc
    21841 atgatacggc tgattagcgt cttaaatgcc tgcggtcggc cgattgcggc ggtcgatatc
    21901 ccctccggcc tcgattgcga tacaggcctg ccgctgcctg tgtgcatcga agcagcggcg
    21961 acaattacgt ttgcggctat caaaaagggc tttactgttt gtcctcagtc ccgtcaggca
    22021 accggacggg tatttgtggc cgatatcggg attgtaccgg catccatcag agcgacggat
    22081 tcttattgag cagcgtgtcg tagccgataa ggtacataaa ccgcttctga ttgaagaccg
    22141 gaatacccag attgttggct tttttcagca gcccatcata ggcctgaacc ttctgcatcg
    22201 acagctcata ccgctgctgg gcaagggggt ctatatccaa gtcatcctgt gtcggacgcg
    22261 gcagtgcggc cggcgcagaa ccgatgatca gaaaatccgt atcaacggtt atatcttcca
    22321 cgacgcgtcc tccccagcgc tcaatcagtt cgcgaattcg ctgggcaccg tcggcatcaa
    22381 tgcgtccgtc gttgttgaaa tcaaactccc cggctaccac aaactggttg gtgcctttgc
    22441 ggtcccaaat caggttgacg acaaggtctt ccttgacgac aggattcttc ttgtccgatt
    22501 tgatgatccg cgctgcgctg atctgttcgg aaacctgaaa gacctctatc tcggccttgt
    22561 tctggccttc ttccgagaca ggtttattgc ggtcatagat tgcaaatgta agcccgcgat
    22621 agacatggtc gttaatgcct gcatccaagt acacgatgcc gttctgcagg tccacgcgaa
    22681 taatctgggc atcgggctga aaggcctgca cttcacggtc aggtttgggc ttaatggctt
    22741 ccagcttgga catggcattt ttcaacagca tatccgtctg ccccagcttc ttttcggttt
    22801 cctgcaggtc cagttgcttc tgccgcagct tggcctgttc ttcttccagt ttgttctgga
    22861 aagtctgcac ctgttctatt acagaatcct gcatgctctt ctggagcgaa tcaaaacggt
    22921 tgcgtatgac attcatctct gtctggaact ggtccagttc ggcaatgaat ttctgccggt
    22981 ccagctcgga tttggcggcc gcatcggcca agtcgctctg gagggtatcg ttgatgcttt
    23041 gcagattggc catctgctgc cgcgccccgt cgagcttctg tttgagatct tcaatcgtct
    23101 tcagcagcgc tacgccgttg agaccgacgg caggattgag atcctgaccg agaacgtttt
    23161 tgttcaaggc atcgattttc atcgagattt cgttgaattt cactgtggcc ggaatatcag
    23221 ccgacggctg ctgaccgaga ataaacgcat acaggtcatc cactactttc tgcatcgttc
    23281 ccagataggt cttgccttgt tcgggtttgc cgacgattcg gaccaaagcc gctttttcag
    23341 agggattggc gatttgatta agatcctctt tggcctgttc ccactgggtg cggtattctt
    23401 cggatttgat gtagtaaaca acggcaaaaa ctgccgcgat gataaacaat gcgacaaagg
    23461 taatcagcgc atacatcatt gtattgcctg aagaagactg gcgaacagcc atattcccga
    23521 ctccttaaaa agggactaat gatacatatt attccgcatt catctgtcag ccgaagactg
    23581 ccggcgtttt tgtagtatcc gttgtcggac aggattcgtc aagaaaaact tgtgtaaatt
    23641 caggccgtac agctataata gtgtcaatgg tattgggatg gagtgttcta tgcagcaggt
    23701 tggaccggaa caattcggca agtggctggc ggtgatgggg ctgggattga cggcagcggg
    23761 tctttttatg ctgatgctgg ctaaaatcgg cttgttccgg cttccgggtg accttcgttt
    23821 cgagggggaa aactggaaga tctttttccc gctgacaagc tgtttggtga tatctgtgat
    23881 tgttacagtg gtattttggc tggtgcagta tttcgtccgt aaataggcgg tatatgacta
    23941 aaaagatgca aaaaatcaga aaagaagatg cttccgccac gataggtctt gtacggctgc
    24001 aaagaatact tgcctctgcg ggtattgact cccgacgcaa atgcgaagaa ctcattcgcg
    24061 aaggggcggt taccgtcaac ggccggatta ttcaggaatt gccggctttt gcagatccgc
    24121 agcacgatga catacgggta aacggccagc ggattcgaaa gccccagaaa gtctatttcc
    24181 tcctcaacaa gcccaaaggc gtgatttgca cgaatatgga tccccagcac cgcaagcgtg
    24241 ccattgatct tatcgattgt tcagaacgta ttttctgcgt cggccggctg gatgccgata
    24301 ccaccggcgc tattattctt accaacgatg cggaactcac caacaagctg acgcaccccc
    24361 gctatgaact gccgaaaacc tatcaggttg ttgtgcgcgg acgaatggaa ggagaagatg
    24421 tagaaaagct caaaaaaggc gtctggcttt cagacggcaa aaccggtgct gcttccgtaa
    24481 aaatattacg taaaaccaat ctggagacaa tgctgcaaat cactatccgc caaggcctca
    24541 acaggcagat tcggcgaacg ctggcggcct tgggctataa agttaaatcc ctcaaacgca
    24601 cccagattgg cgatattgtt ttgaaggaca ttcctgtggg cggatacaag cggctgactg
    24661 ctgcacaaat tgcctatttg aagcgtgcga cagagtctgc cattgacaga caagcttaaa
    24721 aggctggccg caggataacc gatttctgtt ggaaagaata tctgtgattt tggcctgctg
    24781 aatacggaat tatcggtcgg aaaatgacaa aaatgttgaa accagccggc ggcggcattc
    24841 tggaacaaaa aattttttat tttttttaaa aattactgtt gacgaaacgg caaaagtaaa
    24901 taaactgtaa tttcttgatg cgggtcaaac agataaagtt ttccggctga gccgaaaaaa
    24961 aagacgtttc agacgttttg aagaaaaaat tacaaaaatt aaaattttgt acttgaccgc
    25021 tcaaaagtgc gagagtaaca tctcgagtct tgaaattcag caatgtcgct gattactttt
    25081 gagcagcggc attttttatt gctcttttga gaagtaaaga atgagttgag tcttatagag
    25141 ccggccgggc cggggcagca gtgcaccgga aggtgggctt tgcaggtcgg gctcatagct
    25201 cagctggcta gagcgcacgc ctgataagcg tgaggtcgga agttcaagtc ttcctgggcc
    25261 cattgaattg catatcggtg attgacgatt gatgcgtggg ccagcagccg acggcacagc
    25321 aggcagacgt ctctggatgg aatgagtcaa tcgtcagata tcggttggca gcaattcggg
    25381 gacgtagctc agttgggaga gcggcggctt tgcaagccgt aggtcgagag ttcgaatctc
    25441 ttcgtctcca gtggactcaa tatcaaaaac gagtttgtca gctgtcagta ccaagcaatg
    25501 gactggcggc tgctgacaat aagctctttg acaagtgcat agcggatatt tctgtagaat
    25561 aagcaattgg gttgtgtatt ctgtacacat ccagctgcaa tttcaacaga cagaattagc
    25621 aataattctg gaatgagcgt ttgtacacca aacctaagaa caatgagcat gaaagctcat
    25681 taggcgaagc cagtcaatct gtgattgata tggtcaagtt acaaagagtg tatgggggat
    25741 gtcttggtgt cgagaggcga tgaaggacgt ggcagactgc gataagtccg gggcaggagt
    25801 ctagcatccg ttaatcccgg aatgtccgaa tggggaaacc cggtgccggc gggcaaccga
    25861 ccggtatcat ccgtggctga atgcataggc catgtgaagc gaacgcaggg aactgaaaca
    25921 tctcagtacc tggaggaaag gaaatcaacc gagactccgt cagtagcggc gagcgaaagc
    25981 gga
//
LOCUS       DAOTBO010000030        25062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1630_length_25062_cov_5.504619, whole genome shotgun sequence.
ACCESSION   DAOTBO010000030 DAOTBO010000000
VERSION     DAOTBO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 25062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25062
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_25062_cov_5.504619"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLE88_05430"
     CDS             <1..677
                     /locus_tag="PLE88_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75939.1"
                     /translation="AAADDGRPLCRWHELETLPLYGNGPENTSDPNAVITAKLTAADT
                     SPLHLDLIQEPAVLDYICTINRPDSGTLPGRININTAPLHVIAAAIPPVLADPNAADP
                     DKTVTFSSLQLAEEIIQHRPYAKLADLLTKVPRMKQYSDGPWKEENVGMQSISDDIEE
                     EHWILSNLANKFTVRSDVFTAYILVRLGEDGPQRRMIAVFDRSQVWTKNDRPKLVALH
                     PVADPR"
     gene            781..1737
                     /locus_tag="PLE88_05435"
     CDS             781..1737
                     /locus_tag="PLE88_05435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOM75940.1"
                     /translation="MRILITGGAGFIGSHLAEACLREGHSVTVIDDLSTGSLKNIASV
                     EHHPDFEFVCDSVRNPNTMHLLVEKCDVIYHLAAAVGVQLIVDRPVHTIETNIHGTEV
                     VLSVANKFRKKVLIASTSEVYGKSEAIPFHEDDDTVLGSTRFSRWSYACSKAIDEFLA
                     LAYHQQYGLPVVIVRLFNTVGPRQTGQYGMVIPRFVARALKNEPIQIYGDGRQSRCFC
                     CVHDVTDGLMRLMNCRQAEGKVFNLGSDAEITIDALADKIIGLTQSRSIKQYLSYEEA
                     YGRPFDDMKRRVPSLERIRTAIGFVPRYTLDQTLELIIEDLK"
     gene            1848..2720
                     /locus_tag="PLE88_05440"
     CDS             1848..2720
                     /locus_tag="PLE88_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOM75941.1"
                     /translation="MKRCRIFRVVLIAVFMAAMAGCTTVPITGRSQFNTVPDPLINSM
                     ALQEYNSFLKDPETKLSTDPDKTAMVQRVGKRIADAVERYMNENYMNDRIAGYKWEFN
                     LVESKEKNAWAMPGGKVVVYTGLLEVTQDDAGLAVVMGHEIAHAIAKHGAERMSQALM
                     VTLGGIGLSQAMKDKPEATRNIFLSSYGVGASLAVILPYSRLHEKEADQLGLIFMAMA
                     GYDPEAAVGFWERMAASKEGSQIPEFLSTHPADQTRIQSIKEYLPEARRYYKPQGQQA
                     PQSQQTGKLGVYGL"
     gene            complement(2810..3355)
                     /gene="hpt"
                     /locus_tag="PLE88_05445"
     CDS             complement(2810..3355)
                     /gene="hpt"
                     /locus_tag="PLE88_05445"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOM75942.1"
                     /translation="MQFERILATSEQLDQIVSDLAEKIAETYRGRRNAAAVVMLEGAR
                     RFASDLLAKLDFTMDVIFVKASSYYGQTVSTGFVQIEGPQDLSQLVRGKNILLIDDIY
                     DTGQTMATMLSRLSEYQPESIKICVLLEKTIPHIQNIPIDFTGLKVDDAFIVGYGLDY
                     NGCFRELPYIAALSGIPAAER"
     gene            complement(3357..4178)
                     /locus_tag="PLE88_05450"
     CDS             complement(3357..4178)
                     /locus_tag="PLE88_05450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOM75943.1"
                     /translation="MGKHDITILFEDEQIILINKPAGLSVTADRSGAPDVVMMLNKQL
                     RLTEPLRLVHRLDKDTSGILLIAKNKTAQSRYSRLFARRTVQKLYLAIVKGPLPYAGG
                     SIKDPIARSRRNPQAMQVHPRLGKPAHTVWKQLADFGAVSLVCAQTVTGRTHQIRIHF
                     AHRGMPLAIDALYGSAEPLMLSAFKPDYQAKQDKDEPPMISRLTLHAYQLTLPIGQEE
                     TLKTFTAPLDKKFAAVIKLLAKHTAKLAPGFVNQDDLRQILNANPLHYLLADTGE"
     gene            4323..6392
                     /locus_tag="PLE88_05455"
     CDS             4323..6392
                     /locus_tag="PLE88_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM75944.1"
                     /translation="MGILDIIVFVVFVVCVVGVGLYKSRNEQSGEDYFLAGRGLKWWL
                     IGFSLIAANISSEQFVGMSGNAASHVGMAIASYEWMAAITLVVVAFGFLPYFLKAGIY
                     TMPEFLEYRYNPLARVIMAVSMLLIYMLLLGAVTYSGALTINTMAEEMGMKVVPTVAQ
                     ASLVIGIIAMIYVAAGGLKACAWADLIQGSALILGGGVVMYFAFKMLGSINEAAYIDD
                     VATGAVAFKSFEPNQGAMERFVELNRVRLNMILPKNDSVLPWTALLLGLWIPNFYYWG
                     LNQYITQRTLGSASLAEGQKGIVFSAFMKLLIPFVIVIPGIISFNLFASEMKSKAVND
                     NAPVIARYQKANPATTLVQVVEAPDAQSVENWAEGKFMIALYPTVQSQKAARTKNPYV
                     LPMVKADYDKLAVRPYSVFKSDDKSMKTVFGPLAAEMEAYNKATEQAAKAANAQIHTE
                     KFIGYKYDSALGQLLSKVLPRKTGWVGFVLAALLGAIVSSLAAMLNAASTIFTMDVFK
                     KYLNPNASQKTIVLLGRICVVVFSVIAVTLAPALGNPKISHSIFTIIQESQGYLSPGV
                     LAVFVFGLVVRIGPAICGTAGLVTNFIVYGGLKYVSTITRLMENKAMETLIGNFLNRM
                     AISFVACLLVMTIITVLKPLKEPVEFRHNTSIELTPSKGAMIAGVIVVILTLILYAIF
                     SPIGIAR"
     gene            6407..7648
                     /locus_tag="PLE88_05460"
     CDS             6407..7648
                     /locus_tag="PLE88_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75945.1"
                     /translation="MDIATDKQAFAYLKELAASKGMKMDGAKVRRSSSRFCLGVEHGD
                     YNGTELFGVGTDRFIWMAYKPNGTNTMRLFSGNFPQDGIVEFEIGRTPAPKSPEVSDT
                     WARFPYGVDFILRREGYRIAKGIDAVLYGNIPGGGMSRSASLTLNLILSILDANGIAE
                     KDSMKIVDLAQAVENDYIGSPCGKLDQIMILFAKEGKGTHYIPAARSIQYVPLGAGAK
                     DFRIVVMDTGTVRPGLEKSTYKIRRAECEELVKMAQEAGFRITCLADVRDDALFGRIK
                     SHFEKSHPHLVSRMTYIYKAQKRFYRMMDAWKAGDIETVGSVFRQDGIGLRDEYVISG
                     PELETMCDIVRTIPGVLGERMLGGGDKGAAGCLVLAADADRVRQVVETAYPRSRPDFA
                     DKYAVHTCKVVDGIVLFENAI"
     gene            7786..9306
                     /gene="nifA"
                     /locus_tag="PLE88_05465"
     CDS             7786..9306
                     /gene="nifA"
                     /locus_tag="PLE88_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HOM75946.1"
                     /translation="MAKSLAKEVVLLADIAKAFAESLNLEATLESILKALESHLELER
                     GAITLLDPETETIGIKVAHGLSDASKKQITYKVGEGITGLVVQTGKEVVVPDISKDPR
                     FLAKTGQRPRSKGQKIAFFCVPIKLEGRAIGAISVDRKVRPADDFDAHVRLLEVIATM
                     VAQAVKLNKLVESDRRELKDENERLLRELKTRFNIHNMVGTSGAMQEVYRLIEQVANS
                     NATVLIRGESGTGKDLVAHAIHYNSYRSAKPFIKVNCTALPENLLESELFGHEKGAFT
                     GAVERKPGRFELAEGGTLFLDEIGDFSLHLQVKLLRVIQFKEYERVGGYETLKANVRI
                     IVATNKNLEEEIKNGLFREDLYYRINVFPIYMPPLRERKNDVMLLADYFLEKYARENH
                     KNITRISTPAIEMLTSYHWPGNVRELENCIERAVLLCNEDVIRSEHLPPSLQIAKKTV
                     ETGTYTLPEKIEYLEREMIVDALKKTQGCQRHAACELGITERMLGYKIRKYNILWK"
     gene            9531..10295
                     /locus_tag="PLE88_05470"
     CDS             9531..10295
                     /locus_tag="PLE88_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75947.1"
                     /translation="MNKAVLFWMGFVSALAISADAQEEKAVFSVTSDFNSKYVWRGHN
                     LVDDWVWQPSIGLSKGAWSAGIWGNLDMTDENSQSGEFTEYDFYGSYTFSITEQLSLS
                     FGYIYYRFPSAGSTQEVYSSLVWDTILSPSLTVYYDFDDINGTYITTGISHSIEKVME
                     LTDSIPVGLDLSCNVGWGDSNYNEGYWLNGSSEAIRSSGWNDLFVTVGFPMPLGKWTL
                     TPSLNYVTILDSDIRKGLVSDDRDLFFTGFSLSAEF"
     gene            10707..12071
                     /locus_tag="PLE88_05475"
     CDS             10707..12071
                     /locus_tag="PLE88_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOM75948.1"
                     /translation="MYAKKCLSSFSFRPLLGIVLGLICSPVVMAAEGEPPLEGLYQAV
                     DTVWVLIAAFLVFFMQAGFGMVEAGFIRAKNTCNILTKNFLDFCMASLGFFLVGYALM
                     FGDGNGFIGTKGWLLMGLDETVNGLPLYAFWLFQAAFCGAAATIVAGGMAERMNFRAY
                     LIYSFLISAFIYPLIGHWIWGGGWLSKMGFMDFAGSTVVHTTGGIAALVGTILLKPRL
                     GKYGIDGKPRAIAGHNIPLASLGVFILWFGWFGFNPGSTLGITRGLAATASRVALNTN
                     MAAALGGIAALGMVWMRFGKPDLSMAMNGALAGLVAITAPCAFVEPWAACLIGISAGI
                     IVVLGVELLDKLQIDDPVGAVPVHGICGIWGTLCVGIFGKSSLGLPNEGFIYSGNPMQ
                     FGVQMVGSIATIFFVLLTMEIVFKLIDLTIGLRVSRIEELRGLDIGEHGMEAYAGFQV
                     FSTT"
     gene            12111..12452
                     /locus_tag="PLE88_05480"
     CDS             12111..12452
                     /locus_tag="PLE88_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOM75949.1"
                     /translation="MKLIIAYIQPHKLEDVKTALVKANVGKMSVTNSLGCGEQMGYEE
                     SYRGIKFEVNLLKKVRLEIAVNEDFVQRTVDAIISAARTGEIGDGKIFIIDLVDCIRI
                     RTGDHGKAAIG"
     gene            complement(12476..13495)
                     /locus_tag="PLE88_05485"
     CDS             complement(12476..13495)
                     /locus_tag="PLE88_05485"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOM75950.1"
                     /translation="MQVAVYSTKPYVEDFFKRANQQCGHKLVFLQEPLTIDTVPLAKG
                     SQAVCIFVNDTADAAVLMKLKEAGIKLVALRCAGFNNVDIEAAQRLGLCVVRVPAYSP
                     YAVAEHTVALMLALNRRIHRAHWRVREGNFSLDGLMGFDLHGSSVGIIGTGRIGEVVA
                     QILHGFGCRLYAYDVNRNPACEKLGVQYAELDAIYRTCSIITLHCPLVPQTYHLIDKA
                     AIDKMQDCVMLINTSRGALIDTKAVIDGLKSRRIGYLGLDVYEEEGDLFFQDLSDQII
                     QDDVFARLLMFPNVIITGHQAFFTHNALQAIAHTTLSNITAYEKGNCQNILTCSRIIK
                     TPASR"
     gene            complement(13755..14522)
                     /locus_tag="PLE88_05490"
     CDS             complement(13755..14522)
                     /locus_tag="PLE88_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HOM75951.1"
                     /translation="MKSIRHISQAALIAGMLMLSACRPAQRDPYAPVLVTPAVQATET
                     LGVSVQNRPIYMTAIGKGPETVLVMAAIHGNEDAGVPLVQQLIQVLRSRTDLHPTHTV
                     LIIPIANPDGYALQSRMNARGIDLNRNFPASNRIDSAVFGQAGLTEPESQILYNLILT
                     RQPARIISIHQPLYCLDYDGPGEEIAQFMSYFCPLPVKKLGSRPGSLGSFAGTEQNIP
                     IITMELAEEDSRLSPHQLWLRYGPALLAAITYPRPPY"
     gene            14838..15500
                     /locus_tag="PLE88_05495"
     CDS             14838..15500
                     /locus_tag="PLE88_05495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOM75952.1"
                     /translation="MHKTIKLGIAASALLILLTGASVMGDDTQPAADRVNTKPETVKD
                     ANDFTLPDLDGKPVQLGSFKGKKIVVLEWVNYDCPFVKAHYSKELPTSTALYLKYKDK
                     DVVWLLINSTYYATAESVKEWAAGLKLALPVLMDTDGKIGRLYKAQTTPHLFVISKEG
                     KLVYEGAFDNAPLGKTPADESYLNYVDRVLEELTNGKEVSIRKTKPYGCTVKYPPSEK
                     SR"
     gene            15550..17124
                     /gene="aroE"
                     /locus_tag="PLE88_05500"
     CDS             15550..17124
                     /gene="aroE"
                     /locus_tag="PLE88_05500"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOM75953.1"
                     /translation="MKLPGIEYDMTYLAVSLYAKDVDSFCIQLSKARSQGAEAIEIRA
                     DALNQPDPESVSRMIQAVQRAGLPVIVTCRDKIEGGFQEIDFSHRLTILRKAILSGAD
                     MVDIELRNFRHPDAYSVLKAALEDSRTRLILSCHNFDGPFDNLNLLYESMLALYPAAI
                     PKLVYYARHINDCFAALNLLKSADTPAIVFCMGPAGQITRILSKKFGAFLTFACLEEE
                     ATAPGQIPVHQMKSLYRWDCLNSQTEIFGVVGNPVCHSLGPVLFNTCFDADSINAVFL
                     PFLVEGEKTEFDAFLDYVGTAAGFGGFSVTMPHKGRALDYAHQHGDYVDGLAETIGSV
                     NTLKIGFNGIISAYNTDYIGAMDSLTAALGGDHRCLHAVKVAVVGAGGVARAVVAGLV
                     DMGARVTIYNRTVSKAQALANEFRCKAAQLDALSQTEAAVLINCTSVGMRPNTEGCPV
                     PEGVLNPEMTVFDTVYNPMETVLLKKARQIGARTVSGAEMFIRQAMAQYRIFIGRDPD
                     EALMRKIVYDRLQAAG"
     gene            17249..18496
                     /locus_tag="PLE88_05505"
     CDS             17249..18496
                     /locus_tag="PLE88_05505"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOM75954.1"
                     /translation="MKKEKVVLAYSGGLDTSVILPWLKETYGYEVVAFAAELGQGSEL
                     AGIQKKALATGAVKCVVKDLRREFVEDYLWPMLKSGAVYENGYLLGTSIARPLIAKHQ
                     VEVAHQTGATAVAHGATGKGNDQVRFELTYMALDPSLKIIAPWKDPQFTLTSREAAVD
                     YAKKHNIPIAQSKKKIYSQDRNLWHISHEGADLEDPWNEPKDSLFVISRPVSKTPNKP
                     DYIEIGFEKGIPVKLNGRAIGGVKLIERLNDLGGIHGIGQADLVENRLVGMKSRGVYE
                     TPGGTILSTAHRALEMLTLDRETAHYKQQVALRYAELVYYGQWFCRLRQALDAFIDVT
                     QENVTGTVRLKLYKGQATLAGVKSPKSLYNPHLASFTMGAEYNSTDATGFIRLFGLPM
                     KVAGIVERQSTASKAGQNKKAKK"
     gene            18518..19693
                     /locus_tag="PLE88_05510"
     CDS             18518..19693
                     /locus_tag="PLE88_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOM75955.1"
                     /translation="MAVDWIWLIFIFVLGACVGSFLNVVIYRLPRGKSLVSPPSACPA
                     CGKPIRFYNNIPLISWLVLKGRCSDCSAPISCRYFIIELLTSLLFGGVYLWFFWFEYR
                     QTGLPGSTPIEKFFTGGWLFYLVVITLAAAFLAASAVDLEMWIIPLEICWFSTMVGLA
                     GAAIAPFLIHQGYALEKLFPSAAYSPKIAALTVGAALGLLVSLAGLYLGIIKPSYEIQ
                     EQQEDHGHPDKEKPEPQFDDRKEILKEVIFLLPVVIGAFSVLKVTQMPAISEKWCSII
                     GYPVISGLLGALAGYFAGCAVVWITRILGTLAFAKEAMGLGDVHLMGAAGAVIGAKWV
                     LLAFFLAPFLGILWALYQAFFQKIRQIPYGPFLSIAVFSVIIFHDWIQRVLTDFYGF"
     gene            19717..20394
                     /locus_tag="PLE88_05515"
     CDS             19717..20394
                     /locus_tag="PLE88_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM75956.1"
                     /translation="MKRTVQKMVFATFLVVTAVMLTGCTNWKKKYNALDVEHQNLKGL
                     YENCVASLESSASEKSQMSQELAKSQQELAELKRQMQQGKTAAQATGFSGDVKVDEAA
                     GTITVTLPNEILFASGKATLKGATSSELDQIYSVLRERYSGKKVDVVGHTDTDPIRKT
                     KDQWKDNWELSAERALTVLRYLVDRGVSPQNIRAVACGESRPVASNSTASGKAKNRRV
                     EIVVHVR"
     gene            complement(20401..22965)
                     /locus_tag="PLE88_05520"
     CDS             complement(20401..22965)
                     /locus_tag="PLE88_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOM75957.1"
                     /translation="MKRRSFDESDRIRLNLERLDIQTRQRTAAEYLFSEAPLMPYAVA
                     FIIGILFADAACRHNRLFWLTAAGALLTAALCFILSFRTPASGRLGLLLFCSCLTVFS
                     LAALRCCSILAIGPNHILHYLGSSQRELATLQGKVISPVIVNAPTADSSIPWLESKSS
                     FYIKTSRLRSSSGWQTVSGIIRVQVNEPIRHVRPGNTVQVYCWLSRFGPPANPGQFDL
                     RRYMHRKGVYLAASVDIREGIEVLDSSSSYLTQVRSVLYRIAANALLDETITTDEDAA
                     SLASALLLGERGGLHPQLLAAFGNTNLAHYISLSGLHIGILAGSLWGLLRTAGLPRRS
                     RAVLCIFLILIYALIVPPRAPTLRAVILSCFFFISVLAARRPSGLNTLALSAVILLYI
                     RPYDLFNAGWQLSFLSVLGILLFYKPVQFYLLNWFFYSFLPYLRFFGMQHFLHSLLEM
                     LAVGISAWLAIAGLLLFYFGKINPLSPLWTVLLFPFVIVILYAGFLKILLSAVLPTAA
                     ALCGLILNLTTDGFEKTVIFLAQINLDQLAFRPSSLWAGFIYFLMAAFYLTPFRYSRS
                     RKTLLGLLVISFLLPALWQKISVSRQNMLEIVCLSVGHGQAIVLSGPAGEHIIFDAGS
                     ITHQHLAASVIVPFLQQKGIAALDAAYLSHGDLDHINAAADLADWIGIRAVYANSILI
                     ESAQRPSVEKELKDRLEQMHKPLRPPAEYRSKKGMHIRSIWPTQEAVSNLSLSENDKS
                     EVFLIEYAGRRILLCGDIEDYAQSELMRRDPPLQADVVILPHHGSTANLQTGFVEHFQ
                     PQVVVASCAANRLATIYRPLPSSGIKAFYTPADGAVTIQIKADGTLSAVGFSNGFN"
     gene            complement(22984..24255)
                     /gene="serS"
                     /locus_tag="PLE88_05525"
     CDS             complement(22984..24255)
                     /gene="serS"
                     /locus_tag="PLE88_05525"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOM75958.1"
                     /translation="MIDIKQIRENPQKYKDGSKAKKFQIDIDRLLEVDKCLLACKKRL
                     QDIATDKNRIGKDIPKLPPEQKNEVLAALSRLKEEEAELNEEIKNLQPEFDALMMQVP
                     QPPAPEVPYGEDDTQNVELRRWGTVRQFDFQPKDHVELGLALDIIDIERGVKLAGTRN
                     YFLKGDGALLHWAVLRFAMDHMIAKGYTPMVVPLLMRDEVMMGTGYFPGSEEQTYQME
                     RDALNLAGTAEVPVTAYHSGEILSQDKLPIKSVALSTCFRREAGAAGKDTRGLYRIHQ
                     FDKVEQVIIGENDEAKSIQYHYEILNNSEEVLQALHLPYRVVNVCTGDLGRGQVQKFD
                     IETWMPSRNGYGETHSASRFYEFQARRMNLRYKTATGRNEFCHTLNNTVIASPRILIP
                     ILELYQNADGSVTIPEVLRPYMFGKERIEPK"
     gene            complement(24305..>25062)
                     /locus_tag="PLE88_05530"
     CDS             complement(24305..>25062)
                     /locus_tag="PLE88_05530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOM75959.1"
                     /translation="CAASTQAVGEAAMLVRHGKADIMIAGGAHSMIHPLGVTGFNRLT
                     ALSTRNDSPTTASRPFSASRDGFVLGEGAAIVILETLESAQQRKVPILAELIGFGSSN
                     DAFRVTDMHEEARGAVQAMQAALNDAGVTIYDIDYISTHGTSTAENDSIETLAIKKVF
                     GDHAKKVPCSSPKSQFGHLIGATGCAELITCIMAIQEQILPPTMNLTDPDPALDLDYV
                     PNCPRRTRVNTVMSESFGFGGQNNVLILRRFSD"
BASE COUNT         6309 a         5704 c         6673 g         6376 t
ORIGIN      
        1 ttgctgcagc agatgacggc aggccgctgt gccggtggca tgagctggaa acgctgccgc
       61 tgtacggcaa cggaccagaa aatacatcgg acccgaatgc tgtcatcaca gccaaattaa
      121 ccgcagcgga tacatcaccg cttcatttgg acttgattca ggagcctgct gttttagatt
      181 atatatgcac gattaaccgc cccgatagcg gcacacttcc cggccgcatc aatatcaaca
      241 cggccccgct gcatgtgatt gctgcggcta taccccctgt gctggctgac cccaatgccg
      301 ccgacccgga taaaacggtt actttttcat cactgcagct ggcagaggaa atcatccagc
      361 atcggcctta tgcaaagttg gcagatctgc tgactaaagt accgaggatg aagcagtatt
      421 ccgatgggcc ttggaaagaa gaaaatgtcg ggatgcagag tatcagcgac gatatcgagg
      481 aggaacattg gattctgtcg aatcttgcca ataagttcac agtccgcagc gatgtcttta
      541 cggcctacat tctcgtacgg ctgggagaag acggtccgca gcggcggatg atcgctgttt
      601 ttgatcgcag tcaggtgtgg actaaaaacg accgtcccaa actggttgcg ctgcatccgg
      661 ttgccgaccc gcgataatct gaatcacaaa aggctgaaac atcaaaacgc aggaattggc
      721 ggtgcaggtt gattaagtga gtttcttttt ggcaaaaaca ctcattctgg agatcgtgta
      781 atgcgaatat taataacagg cggagccggc tttattggtt ctcatctggc agaggcctgt
      841 ctgcgggagg gtcatagcgt aaccgttatt gatgacctct ctaccggctc cctgaagaat
      901 attgcatctg tcgagcatca tccggatttt gaattcgtat gcgattccgt tcgcaatccc
      961 aacacgatgc atctgctggt cgaaaaatgc gatgtgattt atcatctggc cgccgctgtg
     1021 ggagttcagc ttattgttga tcgtccggtg cataccatcg agaccaatat ccacgggacg
     1081 gaagttgttc tgtcggttgc caacaagttt cgcaaaaaag tccttattgc ctctaccagt
     1141 gaagtgtacg gcaaaagcga ggcgattccc ttccacgaag atgatgacac cgttctgggc
     1201 agcacgcgtt tttcccgctg gtcatatgcc tgcagcaagg ccatcgatga gtttctggcg
     1261 ttggcctatc atcaacagta tggtctgccg gtggttattg tccggctgtt taacacagtc
     1321 gggccgcggc aaaccggaca atacggaatg gttattccgc gttttgtcgc ccgggcgctg
     1381 aaaaatgagc ctattcagat ttacggcgac ggccggcaat cccgatgttt ttgctgtgtc
     1441 cacgatgtga ctgacggcct gatgcgtctg atgaactgcc ggcaagccga aggaaaagtc
     1501 tttaacctcg gttctgatgc ggagataaca atcgatgctc ttgccgacaa aattattgga
     1561 ttgacccaaa gccgaagcat caagcagtat ttatcctatg aagaagcata cggccgaccc
     1621 tttgacgata tgaaaaggcg agttccaagt ttggagcgga ttcgaacggc aatcggattt
     1681 gtcccgcgat atacccttga tcaaacactt gagctgatta ttgaagattt gaaataattc
     1741 aggcgattaa agcgtttata tctccagaaa atacgcttgc atctttctgt tttcggttat
     1801 aatagaaagc gttaacggga aagcaatctg taaaaggagt caaatatatg aaaagatgca
     1861 gaatattccg agttgtttta atcgcggtgt ttatggcggc aatggccggc tgtaccacag
     1921 tacccataac cgggcgcagc cagtttaata ctgtaccgga cccccttatc aactcgatgg
     1981 ctctgcagga gtataatagt tttctcaaag atccggaaac caagttaagc actgacccgg
     2041 acaaaacagc tatggtgcag cgggtcggca aacgcatcgc tgatgctgtc gagcggtata
     2101 tgaatgaaaa ctatatgaac gaccggattg ccgggtacaa gtgggagttt aatctggttg
     2161 aaagtaaaga aaagaatgct tgggcaatgc ccggaggcaa ggttgtggtt tataccggtc
     2221 tgctcgaagt aacgcaggat gatgcagggc tggcggtggt aatgggccac gagattgctc
     2281 atgcaattgc caaacacggt gcggaacgca tgagccaagc cctgatggtg acactgggcg
     2341 gcataggtct gtctcaggcg atgaaagata aacccgaagc tacgcggaat attttcctgt
     2401 cgtcctatgg ggtcggagcg tcgctggctg tgattctccc atacagccgt ctgcatgaaa
     2461 aagaggccga ccagcttggg ttgattttta tggcgatggc tggctatgac cctgaagcgg
     2521 cagttggatt ctgggagagg atggctgctt ccaaagaggg tagccagata ccggaatttc
     2581 tcagtacgca tccggcagac cagacccgca tacaaagtat caaagaatac ctgccggagg
     2641 cccgccgcta ttataagccg caaggccagc aggcccccca atcccagcag accggcaagc
     2701 tgggtgtata tgggctttaa tctgcaatta gccggagtct gtaagaatcc tctgatagac
     2761 tgggtatttt aggccgagct ggttggcacc gggtcttttt gtggaatgct catctttcag
     2821 ccgcaggaat accggataat gcggctatgt aaggaagttc gcggaagcat ccattgtagt
     2881 cgaggccata gccgacgata aaggcgtcat ctactttcag acctgtaaaa tcaatcggga
     2941 tattctgtat atgggggata gtcttttcca gtagaacgca gattttaatg ctttccggct
     3001 gatattctga taatcgggaa agcattgtcg ccattgtttg tcctgtatca taaatatcat
     3061 caatcagcag gatattttta cctcggacca gctgggataa gtcctgcggt ccttcaattt
     3121 ggacaaaacc tgtcgaaact gtctggccgt aatagctgga tgccttgacg aaaataacat
     3181 ccattgtaaa gtccagcttt gcaagcaggt cactggcaaa acggcgagct ccttccagca
     3241 taactacggc tgctgcattt cttctgccgc gataggtctc ggcgattttt tcagccaaat
     3301 cagatacaat ttggtccaat tgttcggatg ttgcaagaat tcgttcgaac tgcattttat
     3361 tctccggtgt cggcaagcag gtaatggaga ggatttgcgt ttaatatctg cctgaggtcg
     3421 tcctgattta caaatcccgg ggcaagtttt gccgtatgct tagccagcag tttaataacg
     3481 gcagcaaatt tcttatccag cggggctgta aaggttttca gtgtctcttc ctgaccgata
     3541 ggaagcgtaa gctgataagc atgcagtgtc agacggctga tcatcggcgg ttcatcttta
     3601 tcctgcttgg cctgataatc gggtttaaaa gcagacagca tcagcggctc cgcactgccg
     3661 taaagggcgt cgattgccag cggcataccc cgatgggcaa aatgaatccg tatctgatga
     3721 gtgcgtccgg tcactgtctg tgcacaaacc agtgaaacag cgccgaaatc agccaattgc
     3781 ttccacacgg tatgtgcggg cttgcctaag cggggatgca cctgcatggc ctgcggattg
     3841 cgccggctgc gagcaatagg atcttttatg gagccgccgg cataaggcag cggacctttg
     3901 acaatggcca gatacagctt ctgaacagtc cgtcgggcaa acagccggga ataacggctc
     3961 tgtgcagtct tgtttttggc aatcagcagg ataccgctgg tatctttgtc caaccgatgc
     4021 accagccgca gtggttctgt gagccgcagc tgtttattga gcatcataac cacatccggc
     4081 gcgcccgaac ggtccgcagt aactgataaa ccggcgggtt tgttgatgag aataatctgc
     4141 tcatcttcaa agagaatagt aatgtcgtgt tttcccattg caggattttc gcaattattt
     4201 tgagatttat ttcatcctgt attgacgatt attttattga tataccacaa aaaatgtgca
     4261 taatccacga aataataagg caaccaatta gcaggaaagt actgttttta ggagatcttg
     4321 gcatgggtat tctggacatt attgtatttg tggtgtttgt cgtctgtgtg gtcggtgtag
     4381 gattgtacaa aagccgcaat gaacagtccg gcgaagatta ctttttggcg ggccggggac
     4441 tgaaatggtg gctgattggt ttttcgctga ttgctgccaa tatttcttcg gagcagtttg
     4501 tgggaatgag cggcaatgcc gccagccatg tgggtatggc aattgccagt tatgaatgga
     4561 tggctgctat tacgctggtt gtggtggcgt tcggttttct gccgtatttt ctcaaggccg
     4621 gcatttatac aatgccggag tttcttgaat atcggtacaa tcctttagcc cgggttataa
     4681 tggccgtctc gatgcttctg atttatatgc ttctgctggg agcggttacc tattccggcg
     4741 ccttgacgat caacaccatg gcagaagaga tgggaatgaa agtggttccc acggtggctc
     4801 aggcctctct ggtgataggg attatcgcga tgatttatgt ggccgccggc ggtctgaagg
     4861 cctgtgcgtg ggccgacttg attcagggca gcgccctgat tctgggcggg ggggttgtga
     4921 tgtattttgc attcaaaatg ctcggcagca tcaacgaagc agcgtatatc gacgatgtgg
     4981 cgaccggtgc ggttgccttc aagagtttcg agccgaatca aggggcgatg gagcggtttg
     5041 tggaacttaa ccgggtgcgg ctgaatatga ttttgccgaa aaatgattcg gttcttccat
     5101 ggacggctct gctgctgggc ttgtggatac ccaattttta ctattggggg ctgaaccagt
     5161 atattaccca gcgcacactg ggttcggcct cgctggcgga aggccagaaa ggcattgtgt
     5221 tttcagcgtt tatgaagctg ctgatacctt ttgtcattgt aatacccggc attatttcat
     5281 ttaacctgtt tgcctcagag atgaagtcta aagctgtcaa cgacaatgct ccggtgattg
     5341 cccgctacca gaaggccaat ccggccacaa cactggtgca ggttgtggag gcgccggatg
     5401 cccagtctgt tgaaaattgg gcggaaggaa aatttatgat cgccctttac cccactgtcc
     5461 agagccaaaa ggccgcccgg acaaaaaacc cgtatgtgct gccgatggta aaggccgatt
     5521 atgacaaatt agcagtgagg ccctattcgg ttttcaagtc ggatgataaa tcgatgaaaa
     5581 cggtttttgg gccgctggcg gcggagatgg aagcatataa taaagccaca gaacaggctg
     5641 ccaaggcggc gaatgcccaa atccatacag aaaaattcat cggctataaa tacgattcgg
     5701 cgctgggaca gctgctcagc aaggtgcttc cccgcaaaac aggatgggtt ggctttgttc
     5761 tggccgcttt gctgggagcg attgtcagtt cgctggcagc gatgctgaat gccgcttcca
     5821 caattttcac aatggatgta tttaaaaagt atctcaatcc caacgcctcc caaaaaacca
     5881 ttgttctgct gggccggatt tgtgttgtgg tcttttcggt gattgcagta acactggctc
     5941 cggctctggg caatccgaaa atcagccaca gcatctttac gatcattcag gaaagtcagg
     6001 ggtatctttc cccgggtgtt ctggcggttt ttgtgtttgg cttagttgtt cgcataggac
     6061 cggctatctg cggcacggcg ggtctggtga ccaactttat cgtttacggc ggactgaaat
     6121 atgtatccac catcaccaga ctgatggaaa acaaagcgat ggaaactctc attggcaact
     6181 tcctcaacag aatggctatc agttttgttg cctgtcttct ggtgatgacg attatcactg
     6241 tcttaaagcc gcttaaagag ccggtcgaat tcagacacaa tacctcgatc gagctgactc
     6301 cctccaaagg tgccatgatc gccggggtga ttgtggttat cttgacattg attctgtacg
     6361 cgatttttag tcctattgga atagccagat aaaggagaat acagcagtgg atatagcaac
     6421 cgataaacag gcttttgctt acttgaagga gcttgctgct tccaaaggaa tgaagatgga
     6481 tggcgccaag gtgcggcgca gctcttcccg cttttgtctg ggcgtagagc atggcgacta
     6541 caacggtacc gagctgtttg gtgtcggcac agaccgtttt atctggatgg cctataagcc
     6601 caacggcacg aatacgatgc ggctgttcag cggcaatttt cctcaggacg gcattgtgga
     6661 gtttgaaatc ggcagaacgc ctgccccgaa atcgcctgag gtcagtgata cgtgggcgcg
     6721 tttcccgtac ggtgttgatt ttattcttcg ccgggaaggg tatcgtattg caaaaggcat
     6781 tgatgcggtt ctttacggca atatccccgg cggaggaatg agccgaagtg cctcgctgac
     6841 gctgaatttg attttgtcca ttttggacgc caatggaatt gcagaaaaag attcgatgaa
     6901 gattgtggac ttggcgcagg ccgttgagaa cgactacatc ggttcaccct gcggcaaact
     6961 ggatcagatt atgattctct ttgccaaaga gggcaaggga acgcattaca ttccggctgc
     7021 acgctccatt cagtatgtac cgctgggggc cggtgcaaaa gatttccgca tcgttgtgat
     7081 ggatacaggc actgttcgtc ccggtctgga aaaatcaacc tataaaatcc gccgtgcaga
     7141 atgtgaagaa ttggttaaga tggcccaaga agccggtttt cgtataacct gtctggccga
     7201 tgtaagagat gacgccctgt tcgggaggat aaagtcccat tttgaaaaaa gccaccctca
     7261 tctggtcagc cggatgacct acatctacaa agcccaaaag cgtttttacc ggatgatgga
     7321 tgcatggaag gccggcgaca ttgaaacagt cggcagtgtt tttcgtcagg atgggattgg
     7381 cctgcgggat gagtatgtta tttctggacc ggaattagag acaatgtgtg acattgttcg
     7441 cacgataccg ggtgttttgg gtgagcgtat gctcggcggc ggggacaaag gagccgcagg
     7501 atgtctggtg ctggcagctg atgccgatag agtcaggcag gtcgtggaga ctgcctatcc
     7561 gaggagccgg ccggattttg ctgataaata tgctgtccat acctgcaagg tagtagatgg
     7621 gatagtctta tttgaaaatg ccatttaatc cggaataatt ggaaccggtt ccgtaaaaaa
     7681 aggaaccggt ttttttgttg aacagttgtc tacaaaattg tataatccgc ttaattcaac
     7741 acaatattgt aagtaaccca caggctttca gcagggagaa taaagatggc caagtcgttg
     7801 gcaaaagaag tggtgctgct ggctgatatt gccaaggcat ttgcggaatc gctcaatctc
     7861 gaggcgacgc tcgaatctat tcttaaggcc ttggaaagcc accttgaact ggaaagaggg
     7921 gcaattaccc tgctggatcc agaaacagaa accatcggca ttaaggtggc tcatggtctt
     7981 tccgatgctt cgaaaaagca gattacctat aaagtcggcg aaggcatcac aggtctggtt
     8041 gttcagaccg gcaaagaagt cgtagtcccc gacatcagca aagatccgcg ttttctggct
     8101 aaaacgggcc agcggccgcg ttcgaagggg cagaaaattg cttttttctg tgttcccatt
     8161 aagcttgaag ggcgagccat cggcgctatc agtgtagacc gcaaagtgcg tcctgctgat
     8221 gattttgatg cccatgtccg gctgcttgaa gtcattgcta cgatggttgc tcaggccgtt
     8281 aagcttaata agcttgtgga atcagaccgt cgcgaactta aggatgagaa tgaacgtctg
     8341 ctgcgtgaac tgaaaacaag attcaacatt cacaacatgg ttggaaccag cggggccatg
     8401 caggaggtct atcggcttat tgagcaggtt gccaacagca atgcgacagt tcttatccgc
     8461 ggcgaaagcg gcacaggcaa ggatttagtt gctcatgcca ttcattataa cagctatcgt
     8521 tctgcaaaac cctttattaa ggtaaattgt acggctttgc ccgaaaatct cctcgagagc
     8581 gagctttttg gtcatgaaaa gggtgctttt accggcgctg tggaacgaaa gccggggcgt
     8641 tttgaattag ccgagggcgg aactttgttt cttgatgaaa tcggtgattt ttctctgcat
     8701 ctgcaggtca agctgctgcg cgtgattcaa tttaaggaat atgaacgtgt cggcggctat
     8761 gaaactctga aagccaatgt ccgaatcatt gtggcgacaa ataaaaatct cgaggaagaa
     8821 atcaaaaacg gcctgttccg cgaggattta tactaccgca tcaatgtctt tcctatttat
     8881 atgcctccgc tgcgggagcg aaagaatgat gtgatgctgc tggcagatta ttttctcgaa
     8941 aaatatgcac gggaaaatca caaaaatatc acccgcattt ctactccggc aattgaaatg
     9001 ctgaccagct accactggcc gggaaatgtc cgggaactgg aaaactgcat cgaaagggcg
     9061 gttcttctgt gcaatgaaga tgttattcga tctgaacacc tgcccccgtc gctgcagatt
     9121 gcaaaaaaaa cagtggaaac gggcacatac acgctgccgg agaagattga atatcttgag
     9181 cgagaaatga ttgttgatgc actgaaaaaa acacaaggat gccagcggca tgctgcatgc
     9241 gagctgggca taacagaacg catgctgggc tataagatac gaaagtacaa tattctttgg
     9301 aagtaacaaa ataaacaata atgtagataa taattgtaat aactacatta ttgttgtttc
     9361 ctgcaaatca gcttagatgg tattgatatt ttgtatctca ttgtaaataa ataagataca
     9421 aactaaatat cctgccgcct tttttttgca tatctgttgc atatctctat tttcaaaatg
     9481 ttcggccgcg tcaggccgca ggcagattaa gcacatttca ggagaataaa atgaacaagg
     9541 cggtattgtt ttggatggga tttgtttcag cgttagcaat ttctgcagat gcccaagagg
     9601 agaaagcggt cttctctgta accagtgatt tcaacagcaa gtacgtctgg cgggggcata
     9661 acttggttga cgactgggtg tggcagccct ctattggact atccaaggga gcttggtcgg
     9721 cagggatctg gggaaacttg gatatgacgg atgaaaacag ccagtcagga gaatttactg
     9781 aatacgattt ctatgggagc tatactttca gcataaccga acagctgtcg ctgtctttcg
     9841 gttatatcta ttacagattt cccagtgccg gcagtacaca ggaagtctat tccagtcttg
     9901 tttgggatac catcctcagt ccctctctga cagtatatta tgatttcgac gatatcaatg
     9961 gcacctacat cacaaccggc atcagtcatt ctatcgaaaa agttatggaa ttaacggatt
    10021 ccataccggt cggccttgat ttgtcgtgca atgtcggctg gggcgattct aattacaatg
    10081 aaggatattg gttgaacggc tccagcgagg cgatccgcag cagtggatgg aatgacttat
    10141 ttgtcacagt gggtttcccg atgccccttg gcaaatggac tcttacgccc agtctgaatt
    10201 acgtgaccat ccttgattcg gatatccgca aagggcttgt ttcggatgat agggatttgt
    10261 tttttacggg atttagttta tccgcagagt tttagcaaat cagcatataa atttttcttt
    10321 tctggtttac cggcattcct tctgccgaag gctgaacagg atcgaccaac gtgattggat
    10381 agaggaatga tgcaacacct aaatataatc tgccggtttt aagaattttt cagccgacta
    10441 atatgcgtgt tcgtcccagc cggtaagaat gaatttaatt gcaattgaga ttttaagatt
    10501 tttgcctaaa aaatacaaaa atgtagaaat atttaattaa aaaaacgtaa atgtatcttt
    10561 tgatttctgg ctattgtcat gttggtgttt cctaactatc ggtaatacaa gtacatatag
    10621 catttttagc aaactatgcc tattggcaca aaggttgctc aagcataaat tttagaaaaa
    10681 tgaattacta attgaaaggg gtaaaaatgt acgcaaagaa atgtttgagt tcatttagtt
    10741 tcaggccgct gctggggatt gttctgggat tgatatgctc gccagttgtg atggcagccg
    10801 aaggagagcc ccctcttgag ggcctgtatc aggcggtaga tactgtctgg gttttgatag
    10861 ctgcgttttt ggtattcttt atgcaggccg gcttcggcat ggtcgaagcc ggctttattc
    10921 gagccaagaa tacctgtaac atattgacca agaattttct ggatttctgc atggcctcgc
    10981 tgggattttt tctggtcggc tatgctctaa tgttcggcga cggcaacggg tttataggaa
    11041 ccaagggctg gcttctcatg ggacttgacg aaacggtcaa cggcctgccg ctgtatgcat
    11101 tttggctgtt tcaggcagcc ttttgcggtg cggcggctac gattgttgcc ggcgggatgg
    11161 ccgaacggat gaatttccga gcgtatctga tttattcgtt tttaatttcg gcctttattt
    11221 atccgctgat cggccattgg atatggggcg ggggatggct tagcaaaatg ggttttatgg
    11281 attttgccgg ttccacggtg gttcatacca ccggcggcat agcggcgctg gttggcacta
    11341 ttttgctcaa gccccgtttg ggaaagtacg gcatcgacgg caagccccga gcgattgccg
    11401 gacacaatat cccgctggct tcattgggcg tttttattct atggttcggc tggtttgggt
    11461 ttaatccggg ctctacgctt ggaatcacaa gaggattggc ggcaacggcg tcgcgtgtag
    11521 ccctgaatac aaacatggca gctgccctcg gcggcattgc tgcactgggg atggtctgga
    11581 tgcgcttcgg caaacccgat ttgtcaatgg cgatgaacgg cgccttagcc ggactggttg
    11641 ctattaccgc tccgtgcgcg tttgtcgaac cgtgggctgc ctgcctgata ggaatttctg
    11701 ccggtattat tgtagtgctt ggagtagaac tgctggacaa gctccagatc gatgatcctg
    11761 tcggtgctgt gccggttcat ggaatctgcg gcatctgggg gaccctctgt gtgggaatct
    11821 tcggcaaatc gtctttgggg cttcctaacg agggctttat atacagcggc aatccgatgc
    11881 agttcggcgt ccagatggtc ggcagtattg ccactatatt ctttgtcctg cttacgatgg
    11941 aaattgtttt caaattaatt gatttaacca tcggcctgcg cgtcagccgt atcgaagaac
    12001 ttcgcggact ggatatcggc gaacacggaa tggaggctta tgccggcttc caggtattca
    12061 gcaccacata ggcaaccgat ataaacataa acaacaagga gacagtgaac atgaaattga
    12121 taatagctta tatacagccc cacaaactgg aagatgtcaa aacagcactg gtcaaggcca
    12181 atgtcggcaa gatgtctgtc accaattcac tcggctgcgg ggagcagatg ggatacgaag
    12241 aaagctaccg cgggattaag tttgaagtga atctgcttaa gaaggtgcgt ctggaaattg
    12301 ccgtaaatga ggactttgtg cagagaaccg tcgatgcgat tatctctgcc gcccgcaccg
    12361 gcgaaatcgg cgacggcaaa atctttatta ttgatttagt ggattgcatc cgcattcgca
    12421 ccggggacca cggcaaggcc gctatcggat gacaggacaa tctgcatcgg gaatcctatc
    12481 ggctggccgg cgttttgatg atgcggctgc atgtcaagat attttgacag ttgccttttt
    12541 cgtaggcggt gatattcgac agcgtagtgt gagcaattgc ctgcagggca ttatgggtaa
    12601 agaatgcctg atggccggtt atgatgacat tgggaaacat cagcagccgc gcgaagacat
    12661 cgtcttggat aatctgatcg gacaggtctt ggaaaaacag gtcgccttct tcctcataga
    12721 catccagacc cagatagccg atgcgccggc ttttgagtcc gtcgataaca gccttggtat
    12781 caattaatgc ccctcggctg gtgttgataa gcatgacgca atcctgcatc ttgtcaatgg
    12841 cggctttgtc aataagatga taggtttggg gaacaagagg gcagtgcaga gtgatgatgg
    12901 aacacgtgcg gtaaatcgca tccaattccg catactgaac gcccagtttt tcacaagccg
    12961 gattgcggtt cacatcgtag gcatacagcc tgcatccgaa gccgtgcaga atctgtgcga
    13021 caacttctcc tatgcggccg gtgccgatga tgccgacgga acttccgtgc aggtcgaagc
    13081 ccattagacc gtcgagagaa aaattgcctt cccgcacacg ccaatgggca cggtgaatcc
    13141 gtcgattcag cgccagcatc agggcaacag tatgttcggc tactgcatag ggcgaatagg
    13201 ccggcacacg gaccacacac aaccccagcc gctgggctgc ctcaatgtcg acattattga
    13261 agccggcaca gcgaagagct accaatttga ttcctgcttc tttaagcttc atcagaacag
    13321 cagcgtcagc agtgtcattg acaaaaatgc atacggcttg gctgcctttg gccagcggta
    13381 cagtgtcgat ggtcagcggc tcttgcagaa aaacaagctt atgcccgcat tgttggttgg
    13441 cacgcttaaa aaaatcttct acatatggtt ttgtgctgta aacggcaact tgcatcggtt
    13501 tattccttgt ttctgtttgt acgttgggca gccaaggcgg ttcaatgtca tttctggtgg
    13561 acaggttgcc agcgtatcga gtacactatg aacaactcag atgattcatc tatttcgaat
    13621 tgctgtattc agattgtcaa agtaccaact tcctaatcaa gacataggag gctgacaatt
    13681 ttcccaattc aacatattaa tttaaatcgt attatacaag acgatttgta ttaatagact
    13741 cacaaatatg cagattaata aggcggccgg gggtaggtta tggctgccag caatgccggg
    13801 ccatatcgaa gccagagttg atgaggcgaa agacggctgt cctcttcggc cagctccatt
    13861 gtgatgatgg gaatattttg ttctgtccct gcaaatgatc ccagcgatcc cggcctcgaa
    13921 ccgagttttt taaccggaag ggggcaaaaa tacgacataa actgtgcaat ctcctcgccg
    13981 ggaccgtcat aatccagaca atacagcggc tggtgtatgg atataattcg ggcaggctgg
    14041 cgggttaata tgagattgta gagaatctgc gattcgggct ctgtaagtcc ggcttggccg
    14101 aaaactgcgc tgtcgattcg gttggatgca ggaaaattgc ggttcaggtc aataccccgc
    14161 gcattcattc gggattgaag agcatagcca tccggattgg cgattgggat gatgagaacc
    14221 gtatgggttg ggtgcaagtc tgtccggctt cgcaaaacct gaatcagctg ctggaccaaa
    14281 gggactccgg catcttcatt gccgtggatg gccgccataa ccagtaccgt ttccggccct
    14341 tttccgatgg cggtcatata gatagggcga ttctgtactg aaacaccaag tgtttctgtg
    14401 gcctgtaccg cgggtgttac aagaaccgga gcataaggat cccgctgggc cggccggcag
    14461 gctgacagca ttagcatccc tgcaatcaaa gcagcttgac tgatatgtct aatggatttc
    14521 atcttaattt tgagagccca aaacaatgct tatctgagca attttttatc gagcaaaaca
    14581 gccttcaaac attagaaaac gcatacaatt tttgcacaat cccgcaggaa tttgtgcttc
    14641 ggcatatagt gttggtgctt gctgtggaat ataagccccg ccagcctgcc tcccatagaa
    14701 gcctcaattc tgcagaaatt gtgaatttgc tattgagggg attttagtcc acatatcttt
    14761 tttttcaaac ttatttcatc caaatccgta taaatgcgca aaataatgat cgcccattta
    14821 gcagaaagga tataacaatg cacaagacaa taaaattagg gatagcagcg tcggctttgc
    14881 tgatattgct gacaggggct tcagttatgg gtgacgacac ccagccggct gctgatcggg
    14941 tcaatacaaa gcccgaaact gtcaaggacg ccaacgattt tacattgccc gatctggacg
    15001 gcaagccggt gcagttaggc agcttcaaag ggaagaaaat tgttgtgctg gaatgggtta
    15061 attatgactg tccattcgtc aaggcacatt acagcaagga acttcccacc agtacggccc
    15121 tttatcttaa atacaaggat aaagatgttg tttggctcct tatcaacagc acgtattatg
    15181 caactgcaga gtctgtaaaa gagtgggctg caggattaaa gcttgcgctg ccggttctga
    15241 tggataccga tggcaagata ggcagattgt acaaagccca gaccacgccg catttatttg
    15301 tgatcagcaa ggaaggcaag ctggtgtatg agggggcttt tgataatgct ccattgggta
    15361 aaacgcctgc ggatgaatcg tatctgaatt atgtcgaccg ggtgctggaa gaattaacca
    15421 acggcaaaga ggtttccata cggaaaacaa agccctacgg ctgcacagta aaatacccgc
    15481 cgagcgagaa aagccgttag catatatcct gtaaggcggg catttccggc aactttgggc
    15541 ttgcaatttg tgaaactgcc gggtatagaa tacgatatga cgtatctggc agtttcactt
    15601 tatgcaaaag atgttgacag cttctgcatc cagttgtcaa aggcccgctc ccaaggcgct
    15661 gaggcaatcg aaatacgtgc agatgccctt aatcagcccg atcctgagtc tgtctcaaga
    15721 atgattcagg ccgtccaaag agcgggtttg ccggttattg taacctgccg ggataagatt
    15781 gaagggggtt ttcaagagat tgatttctcg catcgtctga caattttacg caaagccatt
    15841 ctgtcggggg cagatatggt cgatatcgaa ctgcggaatt tcagacaccc ggatgcctat
    15901 tccgtactaa aagcagctct ggaggattcc cggacacggc tgattctttc ctgtcataat
    15961 tttgacggtc cttttgataa cctcaatctg ctgtacgaat cgatgctggc gctgtatccg
    16021 gcggctattc cgaaattggt ttattatgcc cggcatataa atgattgctt tgcggcgttg
    16081 aaccttctca aatcggcgga tacgccggcc atcgtgttct gcatggggcc ggcaggacaa
    16141 atcacccgca tcttatcgaa aaaatttggg gcatttctga cattcgcctg tctggaagaa
    16201 gaagcgactg ccccggggca gattcctgtg catcagatga aatccctgta ccgctgggac
    16261 tgcttaaaca gccaaaccga aatattcggc gtagtgggaa atccggtttg ccattcgctg
    16321 ggaccggtgc tttttaatac ctgctttgat gccgacagca tcaatgctgt ttttctaccg
    16381 tttttggtgg aaggggaaaa aacagaattt gatgcctttc tggattatgt aggcactgcg
    16441 gcgggttttg gcggcttcag cgtaacgatg ccgcacaaag gtcgggccct cgactatgcc
    16501 catcagcatg gcgattatgt cgatggattg gctgagacaa tcggctcggt caataccctc
    16561 aaaatcggat ttaacgggat tataagcgcc tataataccg attatatagg agctatggat
    16621 tcgctgacag ccgcactggg gggggatcat cgatgcttgc atgcagtcaa agtggctgtt
    16681 gtcggggccg ggggggtagc acgggcggtt gtggctgggc tggtcgatat gggcgccaga
    16741 gtaaccattt acaaccgaac cgtcagcaag gcacaggcgc tggctaatga atttcgctgc
    16801 aaagctgctc aattggatgc actgtcccag acagaggcgg ctgtattaat caactgtacc
    16861 agtgtaggga tgcggccgaa tacagagggc tgtcctgttc cagaaggtgt tctgaatccg
    16921 gaaatgacgg tttttgacac ggtttataat ccgatggaaa ccgtactttt aaagaaagcc
    16981 cgtcagattg gggctcgaac ggtcagcggt gccgaaatgt ttatccggca ggccatggcg
    17041 caatatagga ttttcatcgg cagggacccc gatgaggctc ttatgcggaa aattgtctat
    17101 gaccggctgc aggccgccgg ctagcctgta tcgggtttga gtttctgtct gcagaaggtt
    17161 ttcattgctt tttttcctgt atggatatat attttccgac tttattgtga aaaacataat
    17221 ttcagagaat aaaacggatg gcagaaaaat gaaaaaagag aaagttgtat tggcatacag
    17281 cggggggttg gatacgagtg taattctccc atggttgaaa gagacctatg ggtatgaggt
    17341 tgtagcattt gcggccgaat tggggcaggg cagcgagctg gccggcattc aaaagaaagc
    17401 tttggcgact ggtgctgtca aatgtgtggt taaagacttg cgcagggagt ttgttgaaga
    17461 ttacctgtgg ccgatgctca aaagcggggc ggtctatgaa aacgggtatc ttttaggcac
    17521 cagcattgcc cgtcctttga ttgccaagca tcaggtagag gtagcccacc agaccggagc
    17581 aactgcggtt gcccacggcg ccaccggcaa gggcaatgat caggtgcggt ttgagctgac
    17641 ctatatggca ctggatccgt cactgaagat tattgctccc tggaaagacc cccaattcac
    17701 tctgaccagc cgggaagctg ctgtggacta tgccaaaaaa cacaatattc cgatagccca
    17761 gtcgaagaaa aaaatctatt cgcaggaccg taatctctgg catatcagcc acgagggggc
    17821 ggatttagag gatccttgga atgaacccaa ggacagtttg ttcgttatca gccggcctgt
    17881 ttccaagacc ccgaataagc ccgattatat cgaaattggc ttcgaaaaag gcataccggt
    17941 caaactcaat gggcgggcaa tcgggggtgt taagctgata gagagactta atgacctcgg
    18001 cggcattcat ggaattggac aagcagactt ggttgaaaat cgtctggtgg gaatgaaatc
    18061 gcggggtgtg tatgaaacac cgggcggaac gattttaagt acggctcatc gggcgttgga
    18121 gatgctcacg cttgaccgag agactgctca ttacaagcag caggttgcgc tgcgttatgc
    18181 cgaactggtt tattacggcc aatggttttg cagattgcgt caggccctgg atgcgtttat
    18241 tgatgtaacg caggagaatg ttacagggac agtgcggctc aaactataca aagggcaagc
    18301 aacccttgcc ggcgtcaaaa gtcccaagag cttgtataat ccccacttgg ccagttttac
    18361 gatgggggcc gaatacaatt cgaccgatgc gaccggcttt atccggctgt ttggtctgcc
    18421 gatgaaggtt gcaggaatcg tcgagcggca aagcactgct tcaaaagctg gtcaaaacaa
    18481 gaaagcgaaa aaataactcc tgatatagag gcgatttgtg gctgtcgatt ggatatggct
    18541 gatttttatc tttgtattag gggcctgcgt cggcagtttt ctgaatgttg taatctatcg
    18601 gcttccgagg ggaaagtcgc tggtcagtcc gccttcggcc tgtcctgcct gcggaaagcc
    18661 catccggttt tacaataaca tcccgctgat ttcgtggctg gttttgaagg gacgctgttc
    18721 cgattgcagc gccccgattt cctgccggta tttcataatc gagctgctga cttcactcct
    18781 gtttggtggt gtgtacctgt ggtttttctg gtttgaatac cggcagaccg gcttaccggg
    18841 ctctacgcca atagaaaagt tttttacagg ggggtggctg ttttatctgg ttgtaataac
    18901 attggctgcg gcttttcttg cagcttctgc agtagatttg gaaatgtgga taattccgct
    18961 tgaaatatgc tggttttcga cgatggtggg gctggcaggc gcggcaattg ctccattttt
    19021 aatccatcag ggatatgcat tggaaaagct ttttccgtct gcggcatatt cccccaaaat
    19081 cgctgcattg acagttggag cggccttagg actgcttgta tcactggccg gtttatatct
    19141 tggcatcatc aaaccaagtt acgaaatcca ggagcaacaa gaggatcacg gccatccaga
    19201 taaggaaaaa ccagagccgc agtttgatga ccgcaaagag attcttaaag aagtcatttt
    19261 tcttctgcct gtggtgatag gagctttttc agttcttaaa gtgacgcaaa tgccggccat
    19321 atctgaaaag tggtgcagca ttatcgggta tccggtcatt tcggggcttt tgggcgctct
    19381 ggctgggtat tttgcaggct gtgcggtggt atggattacc cggattttgg gaactcttgc
    19441 ctttgcaaaa gaggcaatgg gtttaggaga tgtgcatctt atgggagcgg ctggggcagt
    19501 cattggagca aaatgggttc tgctggcgtt ttttttggcg ccttttttgg gcatcctatg
    19561 ggccttatat caggcatttt ttcaaaaaat ccgtcaaatt ccttacggac ctttcttgtc
    19621 aatcgctgtt tttagtgtta ttatctttca cgattggata caacgtgtct taacagactt
    19681 ttacgggttt tagctgaaat cgaaacggag gtctctatga aacgaactgt acagaagatg
    19741 gtatttgcaa cgtttttggt ggtgacggcg gttatgctga ccggctgtac caactggaaa
    19801 aagaaatata atgcactgga tgtggaacac cagaatctca aaggtcttta tgaaaactgc
    19861 gttgcttctt tggaatcttc ggcttctgag aaaagccaaa tgagtcagga actggccaag
    19921 agtcagcagg aactggctga attgaagcgg cagatgcagc agggcaaaac ggcagcccaa
    19981 gcaaccggtt tttccggtga tgtaaaggtg gatgaagcag ccggaacgat tacggtaact
    20041 ctgcccaatg aaattctgtt tgcctctgga aaagccacat taaaaggtgc caccagttcg
    20101 gagctggatc aaatatacag tgttttgcgg gagcgctaca gcggcaagaa agtcgatgtc
    20161 gtcggccata cggatacaga tccgattcgc aaaacaaagg accaatggaa agataactgg
    20221 gaactgtctg ccgaacgtgc gttgacagtg ctgcgttatc tggttgaccg cggtgtaagc
    20281 ccgcagaata tccgtgcggt tgcctgcgga gaaagccgtc cggttgcttc caattctaca
    20341 gcgtcaggaa aggccaagaa ccgccgggta gagattgtgg ttcatgtccg gtaacagcat
    20401 ttagttgaag ccattcgaaa agccgacggc tgaaagtgtg ccgtcggctt ttatttgtat
    20461 agtaacagcg ccgtcggcag gggtgtaaaa ggccttgatg cccgatgatg gcagcggccg
    20521 gtaaatagtt gccagacggt tcgcagcgca gcttgccacc acgacttgcg gctgaaaatg
    20581 ctcgacaaag ccggtttgca ggttggcggt ggagccgtga tgcggcaaaa tgacaacgtc
    20641 cgcttgcagc gggggatcac ggcgcatcaa ctcactttgt gcatagtctt caatatctcc
    20701 gcagagaaga attctgcggc cggcgtactc aatcagaaag acttccgact tgtcattttc
    20761 ggataaggaa agattagaga cggcctcttg cgtcggccag atggaacgga tatgcatgcc
    20821 ctttttactg cggtactctg ccggcggccg taacggcttg tgcatttgtt ccagacggtc
    20881 tttaagttcc ttttcgacag acggcctctg ggcggattca ataagaatgc tgttggcata
    20941 aacagcccgg atgccgatcc agtcggcaag atcggctgct gcgttgatgt ggtcaaggtc
    21001 tccatggctt aaataagcgg catccagcgc agcgatgccc ttttgctgca gaaagggcac
    21061 aatgacgctg gctgcaagat gctgatgtgt gatggaaccg gcgtcgaaaa taatatgctc
    21121 gccggccggt cccgacaaca caatcgcctg cccatgcccg acagacaagc agactatctc
    21181 cagcatgttt tgccggctga cagaaatttt ttgccagagt gccggcagca aaaaactgat
    21241 aaccagcagc ccaagaagtg tttttcttga tcggctgtat cgaaaaggcg tcaaatagaa
    21301 agccgccatt agaaagtaaa tgaatcctgc ccaaagactg cttggccgaa atgcaagctg
    21361 atccaaatta atctgggcca aaaaaataac ggttttttca aaaccgtctg tcgtcagatt
    21421 cagtatcaat ccgcacaatg ccgcagcggt cggaagaaca gccgacagga gaattttaag
    21481 gaatccagcg tacaagatga cgatgacgaa cggaaatagt aaaaccgtcc aaagcgggga
    21541 gagaggattg attttgccga aatagaacag caaaaggcct gcaattgcca gccaggccga
    21601 tatgccgaca gccagcattt ccaatagcga atgcagaaag tgctgcatcc cgaaaaagcg
    21661 aagatacggc agaaaagaat aaaaaaacca gttcagcaga taaaattgaa ccggtttata
    21721 aaacagcaga atacctaaga ccgacaaaaa agacaattgc cagcccgcat tgaatagatc
    21781 gtaaggccga atatacagca ggatgaccgc agacagtgct aatgtattca gcccacttgg
    21841 ccggcgggcg gcaagaacag aaatgaagaa aaagcagctt aaaattaccg cacgcagtgt
    21901 cggagcccgc gggggaacaa tcagtgcata aatcaaaatc agaaagatgc acagcacagc
    21961 ccgagaccgc ctgggcagac cggctgtccg cagcagcccc cagagcgagc cggccagaat
    22021 tcctatatgc agtcccgata agctgatata gtgggccaaa tttgtattgc cgaatgcagc
    22081 cagcagctgc ggatgcagac cgcctctttc ccccaacagc agcgcagagg ccagagaagc
    22141 ggcatcttca tctgttgtaa tcgtttcatc cagcagagca ttggccgcga tacgataaag
    22201 aaccgaccga acttgggtca gatatgatga agaggaatcg agcacctcaa tgccttcccg
    22261 tatgtcgacg gatgcggcca gatagacccc tttgcgatgc atataccgac ggagatcaaa
    22321 ctgaccggga ttggcgggcg gtccaaaacg gctcagccag cagtaaacct gtacggtatt
    22381 acccggccgg acgtggcgga tgggctcatt gacctgcacg cggatgatgc cggacactgt
    22441 ttgccagccc gaggagcttc tgagacggct cgttttaatg taaaaagaac ttttggactc
    22501 aagccaagga attgacgaat cggccgtagg ggcattgaca atgacaggag aaatcacctt
    22561 gccctgcaga gttgctaatt ctctctggct gctgccaagg tagtgaagaa tatgattggg
    22621 accgattgca agaatagaac agcagcgcag tgctgcaaga gaaaagacag tcagacagga
    22681 gcaaaacagc aaaagcccca atcgccccga tgcaggggtc ctgaaagaca agatgaaaca
    22741 caaagctgcc gtaagcagtg caccagccgc tgtaagccag aaaagacggt tatgccggca
    22801 ggcggcatcg gcaaacagaa tgccgatgat aaaagcaact gcatagggca tcagcggagc
    22861 ttcggaaaaa agatattcgg cagccgtccg ctggcgagtt tgaatatcca gccgctcgag
    22921 attcagccgg attcggtcag attcgtcgaa gctccttcgc ttcacgcccc tgcctccgtg
    22981 cggttatttg ggctcgatcc tttctttgcc gaacatatat ggccggagta cttcaggaat
    23041 tgttacactg ccgtcagcgt tctgatacag ttcgagaata ggaatcagaa tgcggggcga
    23101 agcaatgacg gtattgttga gcgtgtggca gaattcattt ctgccggtgg cagttttata
    23161 ccgcagattc attcgccggg cctgaaattc ataaaaacgg ctggccgaat gtgtttcgcc
    23221 ataaccgttg cgtgacggca tccaggtttc aatatcgaat ttctgcactt gcccccgccc
    23281 aagatcgcct gtgcacacat tcaccacgcg gtagggcaga tgaagcgcct gtagaacctc
    23341 ttcagaatta ttcaggattt cataatgata ctgaatgctc ttggcctcat cattttcacc
    23401 gatgataacc tgctcgactt tgtcgaattg gtggatgcgg taaagcccgc gggtatcttt
    23461 gccggcggca ccggcctcgc ggcggaaaca tgtgctcaag gccacagatt ttatgggaag
    23521 cttatcttgg cttagaatct cacctgaatg gtaagctgtt acaggaacct cggctgtacc
    23581 ggccaaattc agtgcatccc gttccatctg gtaggtctgt tcttcagagc cggggaaata
    23641 gcccgttccc atcatcactt cgtcccgcat caaaagcggg acaaccatag gggtataccc
    23701 cttggcaatc atatggtcca ttgcgaagcg gagaacagcc caatgcagca gagcaccgtc
    23761 gcctttaaga aaataattgc gtgttcccgc cagctttacc ccgcgctcaa tatcaataat
    23821 gtcaagggcc agccccagct ctacgtggtc tttcggctga aaatcaaatt gccgaactgt
    23881 tccccaccgc cgcagttcaa cattttgcgt atcatcctcg ccgtatggga cttctggtgc
    23941 gggcggctgc ggaacctgca tcatcagagc atcgaactcg ggctggagat ttttgatttc
    24001 ttcattgagt tcggcttctt cttctttgag ccgggaaagg gcggccagca cttcattttt
    24061 ttgctccggc ggcagcttcg gaatatcctt gccgatgcgg tttttgtctg ttgcgatatc
    24121 ctgcagcctc tttttgcagg ccagcaggca tttgtccact tccagcagcc ggtcaatatc
    24181 tatttgaaat ttcttggcct tggagccgtc tttgtacttc tgcggatttt cccgaatctg
    24241 ctttatgtct atcatggcgt tgcctctaaa aaaccggata ttcctctctc atatcctgcc
    24301 tacattagtc cgaaaaccgc cgtagaatca agacattgtt ctgtccccca aagccgaagg
    24361 attcactcat cacggtattg acacgggtcc tgcgggggca gttgggcaca taatccaaat
    24421 ccagcgccgg atcggggtct gtcaagttca tggtcggcgg caatatttgt tcctgtatgg
    24481 ccataatgca ggtaatcagc tctgcacagc ccgttgctcc aataaggtgg ccgaattggc
    24541 tcttcggact gctgcagggg acttttttgg catggtcgcc gaagaccttt ttgattgcca
    24601 gcgtctcaat cgaatcattt tcggccgtac tggtgccgtg cgtactgatg taatcgatgt
    24661 cataaattgt tacaccggca tcgttcagtg cggcctgcat ggcttgtact gctccgcggg
    24721 cttcttcatg catatcggta acgcgaaatg catcgttgct gctgccgaag ccgatgagtt
    24781 ctgctaaaat cggaaccttg cgttgttggg ccgattcaag ggtttcaaga atgacaatcg
    24841 ctgctccttc gccgagaaca aaaccatccc ggctggcgga gaagggtctg gatgctgttg
    24901 tcgggctgtc attgcgggtt gaaagggcag ttagccggtt gaatccggtt acacccagcg
    24961 ggtgaatcat ggaatgggct cctccggcga tcataatatc ggccttgccg tgtcggacca
    25021 gcatggctgc ttcgccgaca gcctgtgtgc tggcggcaca ag
//
LOCUS       DAOTBO010000031        24018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1736_length_24018_cov_5.378375, whole genome shotgun sequence.
ACCESSION   DAOTBO010000031 DAOTBO010000000
VERSION     DAOTBO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 24018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24018
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_24018_cov_5.378375"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLE88_05535"
     CDS             <1..971
                     /locus_tag="PLE88_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75960.1"
                     /translation="IKGLKDFHAAAKVFRNHTDQFSADPFGAGQFITNCDWYQVETPA
                     VCRFQMDGPQAGGSGTLSEPGPLKMDVHLLWPQTAEMMVADYPENLGTAKKLWYAVRA
                     DGQTLAEGRFGAWILGADQIDVALEGKTVLELETRVDSLKNKTIFWAEAVIVTADGKE
                     IPLTRLPLQYEGLIPTENFAQDYYGGKICIAGKPRDSAVGANPQKPNEPGLIRVNLSG
                     LHASRLKAVIGGDYPLGPAADHRKTVSFRSRGSEARFIMLIEPYENDSVIAAVLSDGQ
                     MVKTELKDGRVQVLAFQNLDGNPDQVQVEFSEYSNGKRTFYEKAVK"
     gene            1055..2098
                     /locus_tag="PLE88_05540"
     CDS             1055..2098
                     /locus_tag="PLE88_05540"
                     /inference="COORDINATES: protein motif:HMM:NF027693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4986 domain-containing protein"
                     /protein_id="HOM75961.1"
                     /translation="MSRIRKPCTGFDNSVFAALFSFKMSKICAVLAVSMIGAAFAADG
                     WPSGREKIEFKAEPFSLQDVRLTEGPFRDAMLRTQHYLHDLESDSLLEPLNVPREMIY
                     TKNNWFEFPPEEIADSPILVTDGRKPHEWIKPVEGRTLELGTENVGRPSDVELIPYYR
                     LWNCKYAVYWQLTDQDGWEKLQLQLQAQRQARAEQAARRAARQAALKARTIDAVQIGQ
                     PDSETAHEMKSAESRTGENNGRTWRDAGPGGWFEYRLKVLPELPMTLLCTYWGGDSGR
                     IFDILVDGQVIAVQRLDRPKPGEFLEVEYEIPDNLVKNKSSVVIRFDGHNGSITGGLY
                     GCAVLKPSEQLDK"
     gene            2279..4153
                     /locus_tag="PLE88_05545"
     CDS             2279..4153
                     /locus_tag="PLE88_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOM75962.1"
                     /translation="MNPLNRIFAICFIFLVLLLGRPAAWCQTEYSAVSPDEQLQIHFI
                     LQPAGVPAYRIDYLGKPIVLESRMGFQGDFNRDFEVIGTATRTHQSQWINQFGERRIV
                     PDYYQELTVDLQHASGRLLKLIFRAYNEGAAFRYCFPEQQTQTFVFPDERTEFRLPEG
                     TWGYEEHGTEGEYKRSKTADIEPYCERPLTLEYAHGLWASLAEAANFSYPRMLLSPLE
                     GHPGALVSALGGNTSNTSAPNQRHDSTVTLQAGQSTPWWMLVVGRRPGDLLERNYLML
                     NLNPPCALEDTSWIRPGKVMRDTTLTTANSKAIIDFAAAGGLQYVHLDWKWYGSEDAQ
                     TGDATTVRADNLDIQEIIRYGNQNNVGLIVYVDRRQIKKQRDILFPLYQQWGVKGVKI
                     GFIDVGPQAESEWITETVAKAAQHHLMLNIHDGYRAAGNNRTYPNLMTVEGIRGNEHM
                     PTPEHNCTLPFTRYIAGIGDYTVCYYDRRLKTTHAHQLAMAVVSFSPLQWIYWYDRPT
                     MYAGEPEIEFFRQVPTVWDETKVIDGQIGQYAVIARRQGDSWFIGTINNSQPRQLTIP
                     LHFLDANKTYTAAVFYDDQTVMTKTKVGIDRRPVNKQSVIDAPLSAGGGQAVWIRPLP
                     "
     gene            4183..5607
                     /locus_tag="PLE88_05550"
     CDS             4183..5607
                     /locus_tag="PLE88_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOM75963.1"
                     /translation="MVSVVCIFFGAFLSAMAAADTLVVYPAPETIPANDAFTVQVRTQ
                     ENPWRRLFIYAVQVDMHNPQRSSMAFFDFSGTAEVAVEYNQGDIQSARIRPLSYGIHP
                     EIKGRTLLFKLQRPCNIAIEVNGNIFNNLHLFAGPVEPSKPDPNDPNTLFFGPGIHES
                     ASSIRLPSGKTVYLAGGAVVKSKLVCDSVENVRIAGRGVLYQPHRGIEITFSKNIEID
                     GICVINPSHYTVLGGQSQGIAIRNLKSFSSRGWTDGIDLMSCSDVLIDGVFLRNSDDC
                     IAVYGHRWDYFGDAQNITVQNAALWADVAHPVMIGTHGNPDSPEVIENITLKNIDILN
                     HDEPQINYQGCIAMNASDENLIRNIRVEDVRIEDFQQGQLLNLRVTFNRKYAKAPGRG
                     IENILFRNVSYAGQHANLSVISGYNEQRTVRGVVFENLTINGRLIWDQMPGKPAYFAT
                     SDKAGIFVGEHVEAVEFRTDAAVP"
     gene            complement(5685..6689)
                     /locus_tag="PLE88_05555"
     CDS             complement(5685..6689)
                     /locus_tag="PLE88_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM75964.1"
                     /translation="MNISNVAKIAGVSNATVSRVMRGSPNVKPATVMRVKNAIKYLGY
                     TPKTRNRRPAGKGSKSCFPKGLVGVVVYPDSVGETSSPIQIAVISSIEKKLRDQGASM
                     IHSQIIDSLPPLIDRGLIDAMIAVYWPTSLINRFEDVLRHQILTVFVGTESADQWGDH
                     IHSNNKSIGILAAQYLLTKGCRKLCYIGYKGHSANIDRHKGFADTVKSDKRDLMAFAL
                     PDLYNLDEMTSVVERMCQEGLPDGIFVFDDNWLSKLYPLFQQKFGPRFYDIRLIGCNN
                     EEAYLSGLHPQPATIDINPRIIAEHAVRQLKWRCENRSDPSQTIIRIEPTLVLPNNER
                     "
     gene            7275..9473
                     /locus_tag="PLE88_05560"
     CDS             7275..9473
                     /locus_tag="PLE88_05560"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM75965.1"
                     /translation="MKEKEFFSLLNLDRDGLEKVKASVQLEDFQQASVCFLDYMRNRR
                     KPVYYRGWDQRTIRSDYDTAQADQICAGRLVHVDMNDAIDWHTPRMDDMEWLYCLHRH
                     EFLTTLGRAYWYTGNEKYAKAFKWILSDWIDKNPAPDVEWMLEIPSEQARAYFMKQGN
                     WRPIEAGIRLYTAWFPCYYHFLNSPEFTPEFHLKMLRSMVDHARFLRRFYTRHVSYFN
                     VSPNWGLMESNGLAHMGILLPELKEAEDWRNTALSRFEEQLRMQILPDGVHVERCSGY
                     HLVCTFCILQIAELALLNGHRISQTWLDNLEKMINFVLYLMKPQGVYPMLSDGDESDV
                     IGSRASWGLWEDINNLNMLEDSNDLRYVLKAGARLFHRPDMLYMATLGREGTQPSKTS
                     VAFPDGGFYVSRSGWDKGDKYMVLNCGLLGVYDQNCVHGHADALSMDVAAYGKTLIID
                     PGRYMYEGPYRIWFERTEAHNTVCVDGQDQSEMRDGWLFNTKAVPVCKSWVAGRNFDY
                     FDGSHDGYQRLHDPVTHRRRVLFVKPDYWIVVDDLIGIGKHAFDQYFHFPEKTAVQLD
                     EPTLRAVVRYGDGVWLTILPCAAEGISASIVEGSENPIQGWVSYDYGIKMPAPVLRYA
                     KTCPAPARFTVIVYPAEGRPADVEARWMEQDVLEIQRNGSLERVLFSDSGFKAFGEFE
                     FEGQMLYAQFDAGHTLRRVCGAEVSRIVYAEKVILDSTAKHIQQNFEKSF"
     gene            9518..11365
                     /locus_tag="PLE88_05565"
     CDS             9518..11365
                     /locus_tag="PLE88_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOM75966.1"
                     /translation="MVNKQINTQERDRFINAILSKMTLEQKVGQCFTVHWGGSIITPY
                     VMEAIEKLHIGGLRVTPFGQNSRRGKHYHQNLSYDYEFPKGYKKIKENLFIPAAAFFV
                     SPEQYARQLNTLQKAACSRPHGVPLHISIDQEGDISRDFSFAGINLFPSAMGLTSTGD
                     PQIAYEACKAAARQLRAIGVTNIHSPVLDVNINPMNPEINIRAYGDDPEIIAEYALAS
                     IKGFMEGGLVPTAKHFPGRGDSDIDAHYAVPCVGGDWKRLESVELLPYRVLIANGLPS
                     IMLAHTTVPAVDPSMEISTVSKTIVTGLLRETLGFEGVITTDSITMGALMEKYGVGRS
                     CALAFKAGCDLILNKTEDEFRDQGFCELKRFVEDGEVSEEQLNASVKRILTMKYNLGL
                     FDTAGQVDADKAAEPMRDKEIIRIARTAAEKCATVLRDEAHLLPLSPAQTILVIEQQV
                     LDGYCGFDLTCNNKMFNMAMYEQSMNIIGMDTKFKASQEDEQFVMQYIDQADVIVATN
                     YYWRLCPANNTDLIKKLIAKGKDVIVVTNCPYELGAVAEAKTVICTYSVTPESLKVAA
                     EIIFGKTKPQGKWMLRHYLMPVRSKKESSVRLKGGVTETGDNEITQVVF"
     gene            11558..13366
                     /locus_tag="PLE88_05570"
     CDS             11558..13366
                     /locus_tag="PLE88_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75967.1"
                     /translation="MTIYDYSVIGFYLAFMFLLGPIYRNFSKTASDFFCGGGGMLWWV
                     VGSSAFMTTFTAWAFTGGAAKAYETGTFFLVIFICNTAALVFCYFFVVGKYRQMRIIT
                     VMEGVRRRYGRTSEQILTWLLILTKTVYGGAFLYTIAVFMGSIFNVPMGLLILLLGVV
                     ITLMTVIGGSWSATAGDFVQMILVLVITVTMGILTLVKIGGPAAFVQAVPSRHFYWTQ
                     FDRPAVLAAFIITLLVNQLIQMNSLMEGAARFIFVKNARQAKKAVWIQIVGMVVLPLI
                     WIIPPMAAAVWHPHLAAEYPRLNNPSEAAYVAMAMELLPAGLLGLLVCAIFAATVTSL
                     NSQLNIVGGSFVRNVYIRVMRPAASEKEQIFVGRLFMLIYGAVWILLGLAFQQVKGLK
                     LFDMMLLMAASLGLPMAVPLFLGIFFKKTPPWTGWSTMLAGFLPAFFLGLLFQVDGVV
                     QFLWQDPNRTTADLIRYVCRMPDLNASEVADMKIALTTGIVAVICCGWFFLSMLFYRK
                     NDTPYIHQVSEFFQDMARPIGPDEYELDMHANELRQCTVLGNLCLIYGCFVALLVFVP
                     NKPAGRLIILCCGSIITGIGLALRYAAGRSAAKHRQ"
     gene            13584..14780
                     /locus_tag="PLE88_05575"
     CDS             13584..14780
                     /locus_tag="PLE88_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOM75968.1"
                     /translation="MRKGITAALWMCLGAVVSAGIAGDISADGRVDLLDIVMMADQWL
                     DLSGLPSADIAPSGGDGQVNSLDFAVLSSHWLLAAPETSRFADIPAGAFQMGDSFGEG
                     YMDERPVHPVAVRSFYMARCEISNSRFCEFLNAAWSRAAIYISQGVIYGSANHQPYCD
                     TSVSSPLSQIEFNAGRFQVRSKGGRDMSDDPMVQVSWYGAAAWCNWLSEQEGRPVCYD
                     LTAGRCDFAKKGYRLPTEAEWEYAARGGRSGCRYPWGDTLTENQANYFVNPPFGFHLL
                     WNDGIPPFTSPTGFFDGTLKYKADYNWAAAFSSWQTVSGANGFGLFDMAGNVWEWCND
                     WYDQSYYTAEAQTNPTGPAAGTARLLRGGSWSNPAYSCRVSARGCNTPQSREGYYGFR
                     PVLCLD"
     gene            14940..15209
                     /locus_tag="PLE88_05580"
     CDS             14940..15209
                     /locus_tag="PLE88_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75969.1"
                     /translation="MATQTKKRPKTSKPASAVAAENIAGVTQIKKMTRQEIEQKALAV
                     GVAPDTMDTTELIRAIQRAEGYSPCFGTSDGQCPYLDCCFRGNCL"
     gene            15444..16487
                     /locus_tag="PLE88_05585"
     CDS             15444..16487
                     /locus_tag="PLE88_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOM75970.1"
                     /translation="MVEFFKTVIADFWGTLSEMSPYLLFGFFAAGLVGAFINAQQVER
                     HLGGSGFWQVFKASLFGVPLPLCSCGVIPVSMSLYNHGASRGATVSFLLSTPQTGIDS
                     IVVTYGMMGPIFAVFRPLAAFLTGLIGGIFTNVFDHSPQPAAAPKPDCPDCAAGRRPS
                     RLIYALKHGFITLPADIGTAMLAGIIIAALISALVPDDFFAGVLSTAGGGFVAMLAMM
                     AMGIPVYVCATASVPIAVSLIDKGLNPGAALVFLMTGPATNAAALSILWNKLGPRSAV
                     IYLLTTAGCALAGGLVLDAIFRGDISTITHAHGAMLPQWMKTLSAIVLLAALANGIIR
                     SKRRKEKTAPVPA"
     gene            complement(16484..17500)
                     /locus_tag="PLE88_05590"
     CDS             complement(16484..17500)
                     /locus_tag="PLE88_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75971.1"
                     /translation="MNLTDTKYSQYALVREMMDAVNVVGRFNPSQTKTVAAKIASIGK
                     LFLTGEGSSRIFPAKNAIRKCLRWGLPLQICTDGSRQAAEYDLSQFAVFCASNSGRTK
                     EVVLLAKKLAAEGNENRFGLSANANTLLEEACKETFVLTCGWEQAVAATKSVIEQALF
                     YESILWTIRGQDMTAACTALSRQVQTALTLPIAQSVIAAAVEAPTIYFAGYNDGVAEE
                     LTLKTNEITRNKSDYLEGTYAVHGIEEVMDPKDIVFVVDPIEAEIEKFQEVLVKGVGL
                     KVVAIATHPTPFETIQVPDAGELTPYVYLSAGWNLLVEIGLALGINLDKPTRARKVGN
                     ELIV"
     gene            complement(17543..19738)
                     /locus_tag="PLE88_05595"
     CDS             complement(17543..19738)
                     /locus_tag="PLE88_05595"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOM75972.1"
                     /translation="MDTVSILPEDSIGYHFIPAEYIPQGQDEYSRRNQQVGKPLDQWR
                     RLRAHEIELLVKNGNTADKWENILVTDPFDPKRITNTEFFGFVRLGRVRNIVLEHHDL
                     QVPAGITNSRIVACDIGDDAAIHNVRYMAHYIIGERCILVNIDEMHTTNHAKFGNGIL
                     KEGEDEKVRVWLDIMNETGSRKVMPFDGMIAADAYLWAKYRDDLPLQQKLKEITQNSF
                     DSRRGFYGMVGDQCVIKNCRIIKDVKIGAHCYIKGANKLKNLTINSDVHEPTQIGEGV
                     ELVNGIIGYGCHIFYGVKAVRFILANNSNLKYGARLINSFLGDNSTISCCEVLNNLIF
                     PAHEQHHNNSFLIASVVMGQSNMAAGVTVGSNHNSRANDNEIQAGRGFWPGLCTSVKH
                     SCRFASFVLLAKADYPAELNIPLPFCLLSNNASKDQLEIMPAFWWLYNMYAMARNTWK
                     FQNRDKRLRKVQNIEFDCLAPDTAEEIIAARRLLEIWTARAHLRQSGRSENGRSPDQL
                     AALGRQLLSRKENLEGLEVLGENIEKSHRKAVITKVYEGYQAYGDMLHYYAVKNLLGW
                     MQSGGSLNDMRNALQGPCRRVWVNLGGQLVPDEDVLQLRKDIADGKLNSWEAIHSRYN
                     ELWQAYPLEKQKHAYYTLCGLLETDCLTPEQWQAALDKAARIQDFICEQVYQSRKKDF
                     DNPFRQATYRNLDEMTAAIGTVEKDAFITQVREETQRFKQMIDDVKKRG"
     gene            complement(19886..20863)
                     /locus_tag="PLE88_05600"
     CDS             complement(19886..20863)
                     /locus_tag="PLE88_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOM75973.1"
                     /translation="MLTFGSIQLDMPFIQAPLSGYTDYPMRILARRFGCPLTFTGVML
                     DKIALHHKALRKAKFQPQADEHPVGAQLLGDDPEVIAQAACKFIEVGYDLIDLNFACP
                     APKVLRRARGGYRMQQPEFIRQTFRLTRQLVKTPVFMKIRIGYDSSAASEEDFWTICR
                     NAAEDGVDMLAIHGRTVVQKYKGKADWSRIIEAKKRFGQLTIFGSGDVLDAETAVQRL
                     AESGLDGVIIARGAVGNPWIFQEIRALFSGQQKPPSPSLAEQGQIMLEHFEMICQSRP
                     ERKAVPYFRKFLAGYCRRHPERKKVLLALMACKSRQAVIDTIRQLYCLH"
     gene            complement(20927..20997)
                     /locus_tag="PLE88_05605"
     tRNA            complement(20927..20997)
                     /locus_tag="PLE88_05605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20963..20965),aa:Gln,seq:ctg)
     gene            21255..22610
                     /locus_tag="PLE88_05610"
     CDS             21255..22610
                     /locus_tag="PLE88_05610"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOM75974.1"
                     /translation="MAKKASKAAIEQIRAAIRSFPTGPGLYFMKDAQEKVLYIGKARN
                     LRSRVASYFQPSANLAENRSPWIAEMISRTAAVDYLQTESEVDAVLQEARLIKDIHPP
                     YNTDLKDAKTFPYLEITTRQEYPAVYITRNPQDQRSLLMGPFTSAGDLRAVIGVLQRI
                     YRFRTCTLDIRSSDDKRRFFRPCILYNIRQCTAPCGDRVSKEAYRAQIRDLIRFLCSK
                     RSRTLADLKRRMTDAAAALDFETAAMYRDRIRLIEALDKRGSLDRNVQPEVFAGDPSE
                     ALVKLQTLLESSQPVRIIEGFDIAHLAGSAMVGAMVQFIDGRPFKDGYRRYKIRSVKG
                     IDDYACLQEIVSRRYKQAMAGQELWPDLVLIDGGIGQLHAVEEVFSRMHAPLPKLASL
                     AKKQEIVYLHGRTEPLRLPAHSPVRKLLQYVRDEAHRFAQHYHHLLRSRRLTEDETQK
                     D"
     gene            23259..23891
                     /gene="nadD"
                     /locus_tag="PLE88_05615"
     CDS             23259..23891
                     /gene="nadD"
                     /locus_tag="PLE88_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOM75975.1"
                     /translation="MSQQEKIILFGGSFDPIHRGHLEVAAHALHTLGAGRLIFIPARR
                     SPHKRRIAAEGSHRLAMIRLAIEGTNRLDVSDCELRRPEPSYTIDTVGFFRQQAGPQA
                     TLYWLMGADQLEEFGKWHQVGRLLDLCRICVMLRGGYPLPNIRRFAGIFTDEQIGRLE
                     NDLIRTPEIHISSTDIRRQLACGHMPADALHPAVADYIKQHRLYGYKPSE"
BASE COUNT         5602 a         6144 c         6432 g         5840 t
ORIGIN      
        1 atattaaagg actaaaagat tttcatgccg cagcaaaagt atttcgcaat cataccgacc
       61 agttcagcgc cgatcctttc ggtgccggcc agtttattac caattgcgat tggtatcagg
      121 tggaaacgcc ggcggtctgc cggttccaaa tggatggacc gcaggccggc ggcagcggta
      181 ccctatctga accgggaccg ttgaaaatgg atgttcatct gctgtggccc cagacggcgg
      241 aaatgatggt tgccgattat ccggaaaacc tcggaacggc caaaaaactg tggtatgcgg
      301 tgcgggcgga tggacagacg ctggcagagg gacgcttcgg cgcttggatt ctgggggccg
      361 atcaaataga tgtggctttg gagggcaaaa cggttctgga gctggagacg cgggtcgatt
      421 ccctgaaaaa caagacgatt ttctgggctg aggcagtcat tgtaactgcg gatggaaaag
      481 aaattcctct cacccggctg ccgcttcaat acgaagggct gataccgaca gaaaattttg
      541 cccaagacta ttatggcggc aaaatctgca ttgccggaaa accccgcgat tctgctgttg
      601 gagcaaatcc tcaaaagccc aatgagcccg gccttatacg cgtgaactta agcggacttc
      661 atgccagccg attgaaggca gtcatcgggg gcgactatcc cctcggtccg gcagcggatc
      721 atcgcaaaac cgtcagcttc cgcagccggg gcagtgaggc ccgctttatc atgctgattg
      781 aaccctatga gaatgacagc gtcattgccg ctgtgctttc tgatgggcag atggtcaaaa
      841 ccgaactgaa agacggcagg gtacaggtgc ttgcttttca aaacctcgat ggcaacccag
      901 accaggtaca ggtcgagttc agcgaataca gcaatggcaa aaggacgttt tatgaaaaag
      961 cagtaaaata aattacagga aatagcctat tctactgctg ggcagcattg taaaaacaga
     1021 caatcagcat aagccaggga aaggaataac ccatatgtcc agaatccgta aaccgtgtac
     1081 aggatttgac aacagtgtgt ttgcagccct attttctttc aagatgagta aaatctgtgc
     1141 cgtacttgca gtgagtatga ttggtgctgc gttcgcagca gatggatggc cttccggccg
     1201 ggaaaaaata gaatttaagg ccgaaccttt ttcactgcag gatgtccggc tgacggaagg
     1261 accctttcga gatgccatgc ttcggacaca gcattatctg cacgatcttg aatcggacag
     1321 tctgctggaa cctctcaatg tcccccgtga gatgatctat acaaagaaca actggtttga
     1381 atttccgccg gaagaaattg ccgacagccc cattctggtt acagacggcc gaaaacctca
     1441 cgaatggatt aaacctgtgg aaggccggac gctggagttg gggactgaga atgtaggccg
     1501 gcccagcgat gttgagttga ttccatacta ccgtttgtgg aactgcaaat atgccgttta
     1561 ttggcagttg accgatcagg atggctggga aaaacttcag ctgcagcttc aggctcagcg
     1621 tcaggcacgc gccgaacagg ccgcccgccg ggccgcccgc caggctgctt tgaaagccag
     1681 aacaatcgat gctgttcaaa tcggccagcc ggattccgaa acagcacatg aaatgaaaag
     1741 cgctgaaagc cgcacaggag aaaataacgg ccgaacatgg agagatgccg gaccgggggg
     1801 atggtttgaa taccgcctca aggttctgcc ggagcttcca atgacactgc tgtgcaccta
     1861 ttggggcggt gattccggac ggatatttga tattctggtg gacggacaag tcattgccgt
     1921 tcagagatta gaccggccca aacccggaga atttttagag gtggaatatg aaatacccga
     1981 caatttggta aagaataagt catctgttgt catccgtttt gacgggcaca acggcagcat
     2041 cacaggcggt ttgtatggat gtgcggtatt gaaaccatcg gaacagctgg acaagtaatc
     2101 ccgctggccg gcctctgtct gcggacagga cagaagagag acccatgcgt ccttatgcac
     2161 aaaaaactgc tgcgttttaa tgagtccgta tcaagtgccg gcgacgccgt cacagcggtt
     2221 tttgatgacc caacagcagt ctgccgaatg aacacgaaaa gagaaaggac agttgaacat
     2281 gaatccgcta aatagaattt tcgcaatatg cttcattttt ctcgtattat tgctgggccg
     2341 gccggcggct tggtgtcaga ctgaatattc ggcggtatcg cccgatgagc agctccagat
     2401 ccattttatt ctgcagccgg caggcgttcc agcgtaccga atcgactatc tgggcaaacc
     2461 tattgttctg gagtcgcgaa tgggttttca aggtgatttc aacagagatt ttgaagtcat
     2521 cggaaccgcg acgcgtaccc atcaaagcca gtggattaac caattcgggg aacggcggat
     2581 tgtcccggat tattatcaag aactgacagt ggatctccag catgcctcag gacgcctgct
     2641 gaagctgatc ttcagagcgt ataatgaggg tgctgcattc cggtattgtt ttcccgagca
     2701 gcaaacacag acatttgttt ttccagatga acggactgaa ttccgcctgc ccgaaggcac
     2761 atggggatat gaggaacacg gcaccgaagg ggagtataag cggtcaaaaa cagcggatat
     2821 cgagccgtac tgtgagcgtc cgctgacgct tgagtatgcc cacggactct gggcatcgct
     2881 ggcagaggcc gccaatttca gttatccccg catgctgctg tctcctttgg aaggtcatcc
     2941 gggggcgctg gtcagtgcgc tgggtggaaa tacctccaat acctctgcac ccaaccagcg
     3001 ccatgattca acagtgaccc ttcaggccgg ccagagtacc ccgtggtgga tgctggttgt
     3061 gggcaggcgt ccgggcgatc tgctggaacg caactatctg atgctgaatc tgaatcctcc
     3121 gtgtgccctt gaggacacgt cttggatccg gccgggcaaa gtgatgcggg ataccaccct
     3181 tacaaccgct aattccaagg ccattattga ttttgcagct gcaggcggct tgcagtatgt
     3241 ccatttggat tggaaatggt acggctccga agatgcccag accggcgatg ccacaaccgt
     3301 tcgggcagac aacctcgata ttcaggaaat catccgctac ggaaaccaaa acaatgtggg
     3361 tctgattgtc tatgtggacc ggcgtcaaat caaaaagcag cgcgacattc ttttcccgct
     3421 gtaccagcag tggggcgtca aaggcgtaaa aatcggtttt attgatgtcg ggccgcaggc
     3481 agagagcgaa tggattaccg aaactgtcgc caaagctgcc cagcatcatc tgatgctgaa
     3541 tatccatgac ggctacaggg cagccggcaa caaccgcacc tatcccaatc tgatgacagt
     3601 ggaagggata cggggcaatg agcatatgcc tacgccggag cataactgca cgctgccgtt
     3661 tacacggtat atcgcaggca tcggcgacta tacagtctgc tattatgacc gccgtctgaa
     3721 aaccacacat gcccaccagc tggctatggc agttgtgtcc ttcagccccc tgcagtggat
     3781 atactggtat gaccggccga ctatgtacgc cggtgaaccg gagattgagt ttttccggca
     3841 ggtgccgacg gtctgggatg aaaccaaagt catcgacggc caaatcggcc agtacgccgt
     3901 catcgcccgc cggcaaggcg acagctggtt tatcggcact atcaataatt cccagccgcg
     3961 gcaactgaca attccgctcc atttcctcga cgcaaacaaa acatacactg ccgctgtttt
     4021 ttacgatgat cagacggtta tgaccaagac caaagtcgga atcgatcgac gccctgtaaa
     4081 caagcagtct gtgattgatg cgccgctgag tgccggcggc ggacaggctg tctggatccg
     4141 acctctgccg taaaaattcg ttataaatag gagcagaatc ctgtggtatc agtagtatgt
     4201 atattcttcg gcgcctttct ttctgcgatg gccgcagcgg acacgctggt cgtttatcct
     4261 gctccggaga ctatccccgc aaatgatgca tttaccgtgc aggtccgaac tcaggaaaac
     4321 ccatggaggc gcttgttcat ctatgcagtg caggtcgata tgcacaaccc ccagcggtct
     4381 tccatggcct tctttgattt ttcaggcact gcggaagtag ccgttgaata taatcaggga
     4441 gatatccagt cagcccgaat tcgtcctctt tcgtatggaa ttcatccgga aataaaaggc
     4501 cgaacgcttt tgtttaagct tcaacggccg tgcaatatag ccatagaggt caacggaaat
     4561 atcttcaata atctgcatct ttttgccggt cctgttgagc ccagcaagcc ggatccaaac
     4621 gatccaaaca cactgttctt tggccccggg attcacgaat cggcttccag tatccggctg
     4681 cccagcggta aaacggttta tctggccggc ggcgcagtcg tcaagagcaa actcgtatgc
     4741 gattctgttg aaaatgtccg cattgcagga cggggcgtgc tgtatcagcc ccatcgcggg
     4801 attgaaatta ctttctcaaa aaatatcgag attgacggca tctgtgtgat taatccttcg
     4861 cattatactg tcttgggcgg tcagtcgcag gggattgcaa tacgaaatct caaatctttc
     4921 agcagccgcg gctggacgga cgggattgac ctgatgagct gcagcgatgt gctgatagac
     4981 ggcgtctttc tgcgcaattc cgatgactgc attgctgttt acggccaccg ctgggactat
     5041 tttggagatg cccaaaatat aacggtgcag aatgcggcac tgtgggctga tgtggcacat
     5101 ccggtgatga taggcaccca cggcaacccc gacagtcccg aagttatcga aaatataacg
     5161 ctgaagaata tcgatattct caaccatgat gagccgcaga tcaattatca gggctgcatc
     5221 gcaatgaacg cctccgatga aaacctcatt cgaaacatcc gtgttgaaga tgtccgcatt
     5281 gaagattttc agcaggggca gctgctgaac ctgcgggtca catttaaccg caaatatgcc
     5341 aaggcccccg gcagaggcat agagaacatc ctgtttcgaa atgtctccta cgccggtcag
     5401 cacgccaacc tgtctgtcat cagcgggtat aatgagcagc ggactgtccg cggcgtggtg
     5461 ttcgagaatc tgaccatcaa cggccggctg atttgggatc aaatgcccgg caagcccgcc
     5521 tattttgcta cctccgataa ggccggcatt tttgtcggcg agcatgtgga agcagtggaa
     5581 ttccgtacgg atgcagctgt gccgtaggcc aagctgttgc tgctgcatcg gcataactgg
     5641 acacaaccgg aatggtatct gctgtccgtc tgcggtctgt ttatttatcg ttcattattg
     5701 ggcagaacca aggtcggctc gatgcggatg attgtctggg aaggatcact gcggttctca
     5761 cagcgccatt tcagctgccg gacggcatgc tcggcgatga tccgcggatt gatgtcaata
     5821 gtggccggct gcgggtgcag cccgctcaga tatgcctctt cattattgca gccgatcagc
     5881 cggatatcat aaaagcgggg accgaacttc tgctgaaaca gcgggtagag tttgctgagc
     5941 cagttgtcgt caaatacaaa aattccatcc ggcagcccct cttggcacat ccgctcaacg
     6001 accgatgtca tctcgtccaa attgtataaa tcgggcagcg caaacgccat caggtctctc
     6061 ttgtcggatt tcacggtgtc agcaaatccc ttgtgccgat cgatattggc gctgtggcct
     6121 ttgtagccga tatagcacaa ttttctgcac cctttggtta acaggtattg agccgccaga
     6181 attccgatgc ttttattgtt gctgtgaata tggtcccccc actgatcggc tgattctgtg
     6241 cctacaaaca cggttagaat ctggtggcgg agaacatctt caaatcgatt aatcagagac
     6301 gtcggccaat agacagcaat catagcatca atcaaaccgc ggtcaatcaa aggaggcaga
     6361 ctgtcaataa tctgagaatg aatcatggac gccccttgat cgcggagttt tttttcaatg
     6421 ctcgaaatga cggcgatttg aatcggcgag gaggtttcac cgaccgagtc cggatataca
     6481 acgactccaa ccaatccttt gggaaaacag cttttggagc ctttgccggc cggccggcgg
     6541 tttcgagttt ttggtgtata accgaggtat tttatggcat tttttacccg catgaccgtg
     6601 gctggcttaa catttggact gccgcgcatt acccgtgaaa cagtagcgtt tgaaactcct
     6661 gctattttgg caacattgga tatgttcatt tggctattcc gcttatgctg tttaagaaca
     6721 ataaccccat tagagtaggc caaagaacaa catttttcaa atatattttt ttaatttcat
     6781 gaaaaaataa aaatatatca tatgtaataa tctataaccg atttttatat aataagttac
     6841 gatatatgta aaggggcgtc ttgtttcctg tgttttgtaa atacacattg tatttagtgt
     6901 caatatatgg tttttttgag aatataaaag cctatatatt caattaacaa ataatattta
     6961 caagaaaatg tcattataat gttacatttt gatatactgt aaatacttta tatataagcg
     7021 gatatgagaa tataatgtag ttatggcagt ttggagataa aatatattac ataaaaataa
     7081 aatatttcat gaaaggatat aagtaaaatg gtcttttttt tatggaagaa gaaatcaaat
     7141 ggtgataaaa taacgagcgg taattttcat ctggaaacgt aaatatttcc tgttattgca
     7201 gggagcataa attgcaaaga agtgctggat gtttgatctg aaaaattgga atagctttta
     7261 ttcaggacct attgatgaaa gaaaaagagt ttttttctct gcttaattta gaccgggatg
     7321 gtcttgaaaa agttaaagcg tccgtacaat tggaggattt ccaacaggca agcgtttgct
     7381 ttctggacta tatgcggaac cggcgaaagc ccgtttacta tcgaggatgg gaccagcgaa
     7441 caattcgaag cgattacgat acggcccaag ccgatcagat atgtgccggg cggttagtgc
     7501 atgtggatat gaacgatgca attgactggc acacgccccg aatggatgat atggaatggc
     7561 tgtattgcct tcatcgccat gagtttttaa caacgctggg acgggcctat tggtacacag
     7621 gcaatgagaa atatgccaaa gcatttaaat ggattctttc agactggatc gataaaaatc
     7681 ctgcccccga tgttgagtgg atgctcgaaa ttccatcgga gcaggcccgt gcctatttta
     7741 tgaagcaggg caattggcgc ccgattgagg ccggcatccg tttgtacacc gcctggtttc
     7801 cctgctatta ccattttttg aattctccgg aatttacccc ggagtttcat cttaaaatgc
     7861 ttcgttcaat ggtcgaccac gcccggttcc tgcggcgttt ctatacccgt catgtcagct
     7921 acttcaatgt ctctcccaac tgggggttga tggaatccaa tggtctggca catatgggta
     7981 ttctcctgcc cgaattgaaa gaggctgagg attggcgcaa tacggcactt tcacgctttg
     8041 aagaacagct gcgcatgcag attcttccgg atggcgtgca tgtcgagcgc tgttcgggct
     8101 atcatttagt ctgcacattc tgcattctgc agattgcgga attggcgctt ctgaatggcc
     8161 accgcatcag ccaaacatgg ctggacaatc tcgaaaaaat gataaatttt gttctgtatc
     8221 tgatgaagcc gcagggtgtc tatccgatgc tcagcgacgg cgatgaaagc gatgtcatcg
     8281 gcagccgtgc cagctgggga ttgtgggaag acattaacaa tctcaatatg ctcgaagatt
     8341 ccaacgacct gcgctatgtt ctcaaggcgg gagcccggtt atttcaccgg ccggatatgc
     8401 tctatatggc tacgttgggc cgtgagggga ctcaaccttc gaaaacatca gttgcatttc
     8461 ctgacggcgg cttttatgtc tcccgcagcg gttgggacaa aggcgataaa tatatggtcc
     8521 tgaactgcgg cctgttgggc gtctatgacc aaaattgcgt gcatggacac gcggatgctt
     8581 tgagcatgga tgtggcggcc tacggcaaaa cgctgattat cgacccgggc cgctatatgt
     8641 atgaggggcc gtacagaata tggtttgagc gtacggaagc ccacaatacc gtctgcgtgg
     8701 acggacaaga ccagtctgaa atgcgcgacg gctggctgtt caataccaaa gccgtaccgg
     8761 tctgcaagtc gtgggtggcc ggccgaaact ttgactattt tgacggcagc cacgatggct
     8821 accaacggct ccacgatccg gttacgcatc gccggcgggt gctgttcgta aagcccgatt
     8881 actggatagt tgtcgatgac ctgatcggaa ttggaaagca tgcctttgac cagtatttcc
     8941 attttccgga aaagacagcc gttcagctcg atgagccgac gctgcgggct gttgtcaggt
     9001 atggtgacgg tgtatggctg acgattctgc cgtgtgctgc tgaaggcatt tcagcaagca
     9061 tcgtcgaagg cagcgaaaat cccattcagg gctgggtgtc gtatgattac ggaattaaaa
     9121 tgcctgctcc ggttcttcgg tatgctaaaa cctgtccggc gccggcacgc tttactgtga
     9181 ttgtatatcc ggctgagggc cggcctgccg atgtcgaagc ccgatggatg gaacaggatg
     9241 ttctggagat tcagagaaat gggagtcttg agcgggtact cttttccgac agcggtttta
     9301 aggcattcgg agaattcgag tttgaagggc agatgcttta tgcgcaattt gatgccggcc
     9361 ataccctccg gcgcgtctgc ggagccgaag tcagccgaat tgtgtacgca gagaaagtga
     9421 ttcttgactc taccgccaag cacatccagc agaattttga aaagtctttt taatccgatg
     9481 cagcgatcga agcagttttt gtaaaggaaa catccgcatg gtcaacaaac aaatcaatac
     9541 acaagaaaga gacagattca ttaacgccat cctttccaag atgaccctcg agcagaaggt
     9601 cgggcagtgt tttaccgtcc actggggcgg ctcgatcatt acgccgtatg tcatggaggc
     9661 gattgaaaag ctgcacatcg gcggtctgcg cgtaactccg tttggacaaa actcccgccg
     9721 cggcaagcat tatcatcaga acctcagtta tgactacgaa tttccaaagg gctacaaaaa
     9781 aatcaaagaa aacctcttta ttccggcagc ggcatttttc gtctctccgg aacaatatgc
     9841 ccggcagctc aatacccttc aaaaagccgc ctgcagccgg ccccacggcg tgcccctgca
     9901 tatttccatc gatcaggaag gcgatatcag ccgcgatttc agctttgccg gcatcaatct
     9961 gtttccctcg gcaatgggtt taacgtccac cggcgatccc cagattgcct atgaggcctg
    10021 caaagccgca gcccgccagc tccgtgcaat cggggtaacc aatattcatt cgccggtgct
    10081 ggatgtgaac atcaatccga tgaatccgga aatcaatatc cgtgcctacg gggatgatcc
    10141 cgaaattatc gcagaatatg ccttggcatc tattaaagga tttatggaag gggggctggt
    10201 tccgacagcc aagcatttcc ccggccgggg cgattcggat attgatgccc actatgccgt
    10261 cccgtgcgtc ggcggcgact ggaagcggct ggaatcggtg gagctgctgc cttaccgcgt
    10321 gctgatagca aacggcctgc cgagcattat gctggcacac accacggtgc cggcggttga
    10381 tccgagcatg gaaatctcaa cggtatcgaa aacgattgtt accggcctgc tgcgggaaac
    10441 attgggtttt gaaggagtca tcaccaccga cagcatcacg atgggcgccc tgatggaaaa
    10501 atacggcgtc ggaaggtcct gtgcattggc attcaaggcc ggctgcgatc tgattttgaa
    10561 taaaaccgag gatgaattcc gcgatcaggg attctgcgaa ctcaagcggt ttgttgaaga
    10621 cggcgaagtg tccgaagagc agctgaatgc ttctgtaaag cgcattttga cgatgaaata
    10681 caatctgggc ctttttgaca cagcaggaca ggtggatgca gacaaggccg cagagccgat
    10741 gcgggataag gagatcattc gaattgcccg caccgctgcg gaaaaatgcg ccacggtgct
    10801 gcgggatgaa gcgcacctgc tgcctctcag tccggcccag acgatactgg tgatcgagca
    10861 gcaggtgctg gatgggtatt gcggtttcga cctgacctgc aataacaaga tgtttaatat
    10921 ggcgatgtat gagcaatcga tgaatattat cggcatggat accaagttca aggcctcgca
    10981 ggaagatgag caatttgtga tgcagtatat cgatcaggcc gatgtcatcg tggcaacgaa
    11041 ttactactgg cggctctgcc cggccaataa caccgatctc atcaaaaagc ttatcgccaa
    11101 aggcaaggat gtgattgttg taaccaactg tccctatgag ttaggggctg tggccgaagc
    11161 caaaactgtc atttgcacct attcggttac gccggagtct ctcaaagtgg cggcagaaat
    11221 tatttttgga aaaaccaagc cgcagggcaa atggatgctc aggcactacc taatgccggt
    11281 ccggtctaaa aaggaatcgt ctgtcaggct caaaggcggc gtcacggaaa caggcgacaa
    11341 tgaaattacg caggttgtct tctgatgcaa gacccccgtt tgcatcggca aaacagatgc
    11401 agccggtcgg cccccctact gcgggatggg acttgaatga actaatgtaa gaggacagga
    11461 acggatggtc aggtgtttag ccaacccggc cgcaggcttg gatagaaccc ggccggagaa
    11521 ggattgtaca gcctgcatca gcggttaagg agtaacgatg acaatctatg attatagtgt
    11581 gatcggcttt tatctggctt tcatgttcct cctcggaccg atttaccgaa acttcagcaa
    11641 aaccgccagc gattttttct gcggcggcgg cggaatgctc tggtgggttg tcggcagttc
    11701 ggccttcatg accaccttca cggcttgggc ctttaccggc ggtgcggcca aggcctacga
    11761 aaccggaact ttctttctgg ttatttttat ctgcaatacg gccgctcttg tcttctgcta
    11821 tttctttgtc gtcggcaaat accgtcaaat gcgcatcatt accgttatgg aaggtgtccg
    11881 cagacgctac ggcagaacca gcgagcagat tttaacatgg ctgctgattc tgaccaaaac
    11941 agtctacggc ggcgcctttt tatataccat tgctgtattt atgggcagta tttttaatgt
    12001 gccgatgggg cttctgattc ttctgctggg cgtggttatt accctgatga cggttatcgg
    12061 cggttcctgg tcagccacag caggagattt tgtgcagatg attctggttc tggtcattac
    12121 agtcacaatg ggcattttga cactggtcaa aatcggcggg ccggccgctt ttgttcaggc
    12181 ggttccctcc cgccatttct attggactca gtttgacaga ccagctgttc tggcggcttt
    12241 tattattacc ttgctggtca atcagctcat ccagatgaac agtctgatgg agggggcagc
    12301 gcgttttatt ttcgtcaaaa acgcccgcca agccaaaaaa gccgtatgga ttcaaattgt
    12361 cggaatggtt gttctgcccc tgatttggat tatcccgcct atggcggctg ctgtctggca
    12421 cccccatctg gcagcggagt atccccgctt aaacaatccc agcgaagccg cctatgtggc
    12481 gatggccatg gagctgctgc cggccggcct tctggggctg ctggtgtgtg caattttcgc
    12541 tgccaccgta acctcgctca acagccagct gaatattgtg ggcggcagtt ttgtccgaaa
    12601 tgtgtatatc cgggtgatgc ggcctgctgc atccgaaaaa gagcaaatct tcgtcggccg
    12661 gctgtttatg ctgatatacg gtgcggtatg gattctgctg gggctggctt ttcagcaggt
    12721 caagggattg aaactttttg acatgatgct cctgatggct gcctcgctgg gactgccgat
    12781 ggcagtgccg ctgtttttgg gcattttctt caaaaaaacg ccgccttgga ccggctggag
    12841 cacgatgctg gcgggctttc tgccggcctt ttttctggga ctgctgtttc aggttgacgg
    12901 ggttgttcag ttcctttggc aggatccgaa taggacgacg gctgatttga taagatatgt
    12961 ctgccggatg cccgatttga atgcctcgga agtggcggat atgaaaatcg cattgaccac
    13021 cggcatcgtc gctgttattt gctgcggatg gtttttcctg agtatgctgt tttaccggaa
    13081 aaacgacacg ccgtacatcc atcaggtcag cgaattcttt caggatatgg ccagaccaat
    13141 tgggccggat gaatatgaac tggatatgca cgccaacgaa ctgcgccaat gcacggtgct
    13201 gggcaatttg tgccttatat acggctgttt tgtagcgttg ctggttttcg tgcccaataa
    13261 gcccgccggc cgtctgatta ttctctgctg cggcagcatc ataaccggca tcggcttggc
    13321 tttgcggtat gcagcaggcc ggtctgcggc aaaacaccgg cagtaaaaac agattcaaac
    13381 accaaaagac cggacagtgg ataaatacct gctgcccggc gcggattgtt tcgagagttc
    13441 ccccgataat gcaagcagca aacagtttca aatagcggct tttgcaggtc gtcaaatcgg
    13501 atttggcttg attttgcggt aataaattgc tataatatat gcacttaaag aagctgtgca
    13561 tttcgcaatg cggggaacag gtggtgagga aaggtataac agctgcctta tggatgtgct
    13621 tgggtgccgt tgtgtcggcc ggcattgccg gcgatatcag cgcagacggc cgtgtggatc
    13681 ttctggacat tgtcatgatg gccgaccaat ggctggacct gtccggcctg ccgtcggctg
    13741 atattgcccc atccggcggc gacggccaag tcaatagcct cgattttgcc gttctgtcat
    13801 cccattggct tcttgccgcc cctgaaacaa gccgctttgc cgatattccc gcaggagcat
    13861 ttcaaatggg cgacagcttc ggtgaaggtt atatggatga gcggccggtg catccggttg
    13921 cggtccgttc attttatatg gcccgctgcg agatttccaa cagccgcttt tgcgagtttc
    13981 tcaatgctgc ctggagccgg gcggcgattt atatctcaca gggagtcatc tacggctcag
    14041 ccaaccatca gccctattgc gacacatcgg tatccagccc cttaagccag atagaattca
    14101 atgcaggccg ctttcaggtg cgcagcaagg gcggccggga tatgtctgat gacccaatgg
    14161 tgcaggtaag ctggtacggt gctgcggcat ggtgcaattg gctcagcgaa caggaaggcc
    14221 ggccggtctg ctatgattta accgccggcc gctgcgattt tgccaaaaaa gggtatcgtc
    14281 tgcccaccga agccgagtgg gaatatgcag cccgcggcgg acggtccggc tgccgctatc
    14341 cgtggggaga tactctgact gaaaatcagg ccaattattt tgtcaatccg ccgtttggtt
    14401 ttcatctgct ctggaatgac ggcatcccgc cgtttacatc gccgacggga tttttcgacg
    14461 gaaccttgaa atacaaagcc gattacaact gggcggctgc gttttccagc tggcagaccg
    14521 tcagcggcgc caatggattt gggctgtttg atatggccgg caatgtctgg gaatggtgca
    14581 atgactggta cgatcagtcg tattacactg ctgaagccca aaccaatccg accggtccgg
    14641 ccgcaggaac cgctcgcctg cttcgcggcg gaagctggag caatcccgct tacagctgca
    14701 gggtctctgc acgcggctgc aatactcccc aaagccgaga gggatactac ggtttccggc
    14761 cggttttgtg tctcgattaa caaagaattc accgcttcag tcctgaaaaa gggctttcat
    14821 accaaaccaa ttcgcagcat gcatcgtatt gaatttaacg gatttttccc ttgctatact
    14881 atacggatgc tgtaaattat acttgttaga tggatttttt tatcagaaag gacgctaaaa
    14941 tggcaactca aactaaaaaa cgccctaaaa cttcaaaacc ggcttcggct gttgctgccg
    15001 agaatattgc gggtgtaact cagataaaaa agatgacccg gcaggaaatt gaacaaaagg
    15061 ccttggctgt cggagttgcg ccggatacga tggacacaac cgagcttatc cgggccattc
    15121 aaagagcaga agggtatagc ccctgttttg gaacgtctga cgggcaatgt ccatatttgg
    15181 actgctgttt cagaggcaat tgcctataaa ctgcggcttg ctgttctaaa aaaggggtgc
    15241 ttgctgcttg tatagctcct ccagatgtaa tttcaattaa aaaaaagcag agcaggaagg
    15301 tttgtttagt cacatacagg acatattaga tattgtattg tctgtatgcc cccctttttt
    15361 ttcttggaac aaaccaaatg gaatcctcgt agattatttt agtgtaaaac ctatgcaaat
    15421 tatccttatt taagatagag tttatggttg agttttttaa gacagtcatt gcagatttct
    15481 ggggtacatt gtccgaaatg tctccctatc tgctttttgg tttttttgcc gccggactgg
    15541 tgggggcttt tataaatgca cagcaagttg agcggcattt aggcggcagc ggtttttggc
    15601 aggtgtttaa ggcatcgctg ttcggtgtcc cgctgccgct gtgctcatgc ggggtaatac
    15661 cggtttcaat gtcgttgtat aaccacggcg ccagccgggg ggcgacggta tcctttctgt
    15721 tgtcaactcc gcagaccgga atcgacagca tcgttgtaac ctatgggatg atggggccga
    15781 tttttgcggt tttccgtccg cttgctgcct ttttgacggg gctgattggc gggattttta
    15841 ccaatgtttt tgaccattcg cctcaaccag cggcagcccc caagccggac tgcccagact
    15901 gcgctgccgg cagacgcccc agccggctga tatatgccct caagcacggt tttatcaccc
    15961 tgcctgccga tatcggaaca gccatgctgg ccggcatcat cattgcggcg ctgatttcag
    16021 cgctggtgcc ggacgacttt tttgcaggcg tgctttccac tgccggcggc ggctttgtgg
    16081 cgatgctggc gatgatggca atggggattc ccgtttatgt ctgtgctacg gcatcggtgc
    16141 ctatcgcggt atccctgatt gataaagggc tcaatcccgg tgcagcactg gtctttttga
    16201 tgacagggcc ggctaccaac gcggcggccc tgtcgatact ttggaacaag ctgggacccc
    16261 gcagcgcagt gatttatctg ctgacgacag ccggctgtgc cttggcaggg ggacttgtgc
    16321 tcgatgcgat tttccgcggc gacatcagca ccataaccca tgcccacggc gcgatgctgc
    16381 cgcaatggat gaaaaccctc agcgctattg tcctgctggc ggcgctggcc aacggcatca
    16441 tccgctccaa acgccgaaaa gaaaaaactg cccctgtgcc ggcttaaaca ataagttcat
    16501 ttccgacttt gcgggcacgg gtgggcttgt ccagattgat gcccagcgcc agtccgattt
    16561 caaccagcag attccagccg gcactgagat agacatacgg cgtcagttca ccggcatcgg
    16621 gaacctgaat cgtctcaaac ggcgtcggat gcgttgcaat ggcaaccacc ttcagaccga
    16681 cccccttgac cagcacctcc tgaaacttct cgatttccgc ttcaatcgga tcgaccacaa
    16741 acacgatatc cttgggatcc atgacctctt cgatgccgtg caccgcatag gtgccttcca
    16801 gataatcgga tttgttgcgg gttatttcgt tggttttaag cgttagttcc tccgcaacgc
    16861 cgtcattgta gccggcaaaa taaatcgtcg gcgcctcaac agccgcagcg atgacggatt
    16921 gggctatcgg cagcgtcagg gctgtctgga cctgccggga cagcgccgtg caggcggccg
    16981 tcatatcctg cccccgaatg gtccacagaa tggactcata aaagagcgcc tgctcgataa
    17041 cgcttttggt tgccgcgacg gcctgttccc agccgcaggt tagaacgaat gtttctttgc
    17101 aggcctcttc caaaagcgtg ttggcgttgg cactgagacc aaagcggttt tcgttgccct
    17161 cggcagccag tttcttggca agcagaacta cttcttttgt ccggccggaa ttggacgcgc
    17221 agaacaccgc aaattgggaa aggtcgtact cggcggcctg acgggagccg tcggtgcata
    17281 tctgaagagg tagtccccac cgcaggcatt tgcggatggc gtttttggcg ggaaaaatcc
    17341 ggctggagcc ctcgccggtt aaaaacagct ttccgatgga agcaattttg gcagcgacag
    17401 ttttcgtctg cgagggattg aaccgtccga cgacattgac ggcgtccatc atttcccgga
    17461 ccagtgcata ctgggaatat ttagtatccg ttagattcat ctgattgtcc tttcgaatgt
    17521 ccgattatcg tttagctcga catcaacctc gcttttttac atcatcaatc atctgcttaa
    17581 atcgctgcgt ttcttcgcga acctgcgtga taaaagcatc cttctcaacc gttccgatgg
    17641 cggcggtcat ctcatcgagg tttcgatagg tcgcctgccg gaagggattg tcaaagtcct
    17701 tcttgcggct ctgatagacc tgttcacaga taaagtcctg aatccgggcg gccttatcga
    17761 gggcggcctg ccactgctcg ggcgtcaggc aatccgtttc gagcagaccg cagagggtat
    17821 agtaggcgtg cttctgcttt tccagcggat aggcctgcca gagttcattg taccggctgt
    17881 gaatggcctc ccagctgttc agtttgccgt cggcaatgtc ttttcgaagc tgcaggacat
    17941 cctcgtccgg caccagctgc ccgccgaggt tgacccacac ccgccggcag gggccttgca
    18001 gtgcattccg catgtcgtta agcgagccgc cggactgcat ccagcccagc agatttttga
    18061 ccgcataata atgcagcata tcaccatagg cctgatagcc ctcatagacc ttcgtgatga
    18121 ctgccttgcg gtgcgatttt tcgatatttt cgccgaggac ttccagccct tcaagatttt
    18181 ctttgcggct gagcagctgg cgtccgagtg ccgccagctg gtcggggctt ctgccgtttt
    18241 cgctccggcc gctctgccgc aggtgagccc gcgccgtcca gatttccagc agccgccggg
    18301 cggcaatgat ttcttcggct gtgtcggggg ccagacagtc gaattcgata ttttggactt
    18361 ttctgagccg tttgtcgcgg ttctgaaatt tccacgtatt gcgggccatg gcatacatat
    18421 tgtacagcca ccagaaggcc ggcataatct ccagctggtc tttggaagca ttgttgctca
    18481 gcaggcaaaa cggcagcggg atattcagtt cagccggata atcggccttg gccagcagca
    18541 caaaggatgc aaaccggcag gagtgcttga cgctggtgca caatcccggc cagaagccgc
    18601 ggccggcctg aatctcgttg tcgttggccc ggctgttgtg attggacccg accgtcacgc
    18661 cggcggccat gttgctctgt cccatcacaa cagaggcaat cagaaacgag ttgttgtggt
    18721 gctgctcgtg agcggggaaa atcagattat tgagcacctc gcagcaggag attgtggaat
    18781 tgtctcccaa aaaggagttt atcagccgcg cgccgtactt cagattggaa ttgttggcta
    18841 agatgaagcg gacggccttg acaccgtaaa agatatggca gccgtagccg atgatgccgt
    18901 tgaccagttc caccccttcg ccgatctggg tgggttcgtg gacatcggag ttgatggtca
    18961 ggtttttgag cttgttggcg cctttgatgt agcagtgggc gccgattttg acatctttga
    19021 tgatgcggca gtttttaatg acgcactggt cgcccaccat cccgtaaaag ccccgccggc
    19081 tgtcaaaact gttctgggtg atttctttga gtttctgctg cagcggcaga tcgtcgcggt
    19141 atttggccca cagatacgca tcggcggcaa tcattccgtc aaacggcatg accttgcggc
    19201 tgccggtttc gttcataata tccagccaga cgcggacttt ttcgtcctcg ccctctttga
    19261 gaataccgtt gccgaatttg gcatgattgg tcgtgtgcat ttcgtcaatg ttgaccagaa
    19321 tgcagcgttc gccgatgatg taatgggcca tgtagcgaac attgtgaatg gcggcatcgt
    19381 cgccgatatc gcaggcgaca atgcggctgt tggtgatgcc ggccggaacc tgcaggtcat
    19441 ggtgctccag tacgatgttg cgcacccgtc ccagacgcac aaaaccgaaa aattcggtat
    19501 tggtaatccg cttggggtca aacgggtcgg taacgaggat attttcccat ttgtcggctg
    19561 tgttgccgtt ttttaccagc agttcgattt cgtgcgcccg caggcggcgc cactggtcca
    19621 gcggttttcc cacctgctga ttgcgccggc tgtattcgtc ttggccctga gggatgtatt
    19681 ctgcggggat gaaatgatag ccgatactgt cttccggcag gattgaaacc gtatccatct
    19741 gcgttctccg ttttaaaaaa ccctctcaaa atgcctctaa cttcttcatt tataagaaat
    19801 ctcctgcggc ttgtcaaaga ttattgcaaa atgccgcgcc tatttttccc cgccggccgg
    19861 atagccccca tccggccgga ccgattcagt gcaggcagta cagctgccgg atggtgtcga
    19921 tgacggcctg ccggctcttg caggccatca gggcaaggag gacttttttg cgttcgggat
    19981 gccgtctgca gtagccggcc agaaatttgc gaaagtaagg aactgctttc cgctcaggac
    20041 ggctttggca aatcatctca aagtgttcaa gcataatctg cccctgctcg gccagagaag
    20101 gacttggcgg tttctgctga ccgctgaaaa gggctcggat ttcctgaaaa atccacggat
    20161 tgccgacagc cccgcgggca atgataacac cgtccagtcc actttcggca agccgctgga
    20221 cggccgtttc ggcgtccaga acatccccgc tgccgaatat cgtcagctga ccaaaccgct
    20281 ttttggcctc gatgatgcgg ctccagtcgg ctttgccctt gtatttctgt acgacggtgc
    20341 gtccgtggat ggccagcata tcgacgccgt cttctgcggc gttgcggcag atcgtccaga
    20401 agtcctcttc cgacgcggcg ctgctgtcgt agccgatgcg tatcttcata aacacggggg
    20461 tcttgaccag ctgacgtgtc aggcgaaacg tctggcggat aaactccggc tgctgcatcc
    20521 gatagccccc gcgtgcccgc cgcagcacct tcggggcagg gcaggcaaag ttcaggtcaa
    20581 tcaggtcata gccgacctcg atgaatttgc aggccgcctg cgcaataacc tccggatcgt
    20641 cgcccagcag ctgcgcgcca accggatgct catcggcctg cggctgaaat ttggccttcc
    20701 gaagcgcctt atgatgcagg gcaatcttgt ccagcataac gccggtaaaa gtcagcggac
    20761 agccgaaccg gcgggccaaa atccgcatgg ggtagtcggt atagccggac aacggtgcct
    20821 gaatgaacgg catatccaat tgtatggaac cgaatgtgag catctgtcaa aaagtacctg
    20881 tcataaaaaa agccctctgc cgacgcttca acaaagggct tttatactgg ggtacaggga
    20941 ttcgaacccc gactaactga tccagagtca gtcgtgctac cgttacacta taccccatta
    21001 acttctttgt atgcaataac ttctgtaaag cacgacgaac cggtcaacaa tgttttattt
    21061 accttacaat gcggctgttg attttgcaag aaaaataagg gaaattccta agcacaaaac
    21121 cggatccgca aggtgctgta tggcagcggc acgcctgctt tttagcattg ctcagcggtt
    21181 tttttccggc cggcaattgc ggcttccggc ggcgcacaaa ttgccttttc tgataaatcc
    21241 ggtacaatac cgttatggca aaaaaggcat ccaaagcggc aatcgagcag attcgggcgg
    21301 caatccggtc ttttccgacc ggtccgggtc tgtattttat gaaggatgcg caggaaaaag
    21361 tgctctacat cggcaaggcc cgcaacctcc gcagccgggt ggcaagctat tttcagccca
    21421 gcgccaatct ggccgaaaac cgcagcccgt ggattgccga gatgatcagc cgcacggcgg
    21481 cggtggatta tctccagacc gaaagtgaag tcgatgccgt tctgcaggaa gcccggctga
    21541 ttaaagatat tcatccgccc tacaacaccg acctcaaaga tgccaagacc tttccctatc
    21601 tggagattac cacccgtcag gagtatccgg cggtgtatat cacccgcaat ccgcaggacc
    21661 agcgcagtct gctgatgggt ccctttacca gcgccggcga cctgcgggcg gtcatcggcg
    21721 tgcttcagcg gatttaccgg ttccgcacct gcacgctgga catccgaagc agcgatgaca
    21781 aacgccggtt cttccgcccc tgcattctgt ataacatccg tcaatgcaca gccccctgcg
    21841 gcgaccgcgt ttccaaagag gcctaccgcg cgcagattcg ggatttgata cggtttctct
    21901 gctccaagcg cagccggacg ctggcggatc tgaagcggcg gatgaccgat gccgccgccg
    21961 cactggattt tgaaacggcc gccatgtacc gcgaccgcat tcgcctgatt gaagccctcg
    22021 acaagcgcgg cagtctcgac cgcaatgtcc agccggaagt gtttgccggc gacccgtccg
    22081 aagcactggt caaactgcag acgctgctcg aatccagcca gccggtgcgg attatcgaag
    22141 gatttgatat cgcccactta gccggcagcg cgatggtcgg cgcgatggtg cagttcatcg
    22201 acggcagacc gtttaaggac ggctatcgca gatataaaat caggtctgta aaggggatag
    22261 acgattatgc ctgcctgcag gaaattgtgt cccgccgcta taagcaggcg atggcagggc
    22321 aggaattgtg gcccgatctg gtgctgattg acggcggcat cggccagctg cacgccgtcg
    22381 aggaggtgtt cagccggatg catgccccgc tgcccaagct ggcttccctt gccaaaaagc
    22441 aggagattgt gtatctgcac ggacgcacag aaccccttcg gctgccggcg cattcgccgg
    22501 tgcgaaagct gcttcagtat gtccgggatg aagcccatcg gtttgcccag cactaccatc
    22561 atcttctccg aagcaggcgc ttgacagagg acgaaactca aaaggactga agcgtggttc
    22621 ggtatggata aaaaaaaaga agagcttcac cggtggatga gaagctcttc ttttgccgct
    22681 tgagcggagc tgctgctcac actcttattt ctttttagcg acttactttt tggtggcttt
    22741 ctttaccgct ttcttagcag gcgccttctt tgctaccttc tttgctactt tctttgccat
    22801 ggctttgtgt cacctccttt cgactgtttt taaggagttg taatccgttt ttacttttca
    22861 catagcatta ttatacacaa ttatcggcca aaagttcaac cttccttgac aaaaaaacga
    22921 ttctgcgcag cgcagtggcc tgtgtccgat gctgccgccg cgaaaaaaac agcgccgctt
    22981 cttccgcgcc gccgatgcag ccgcgccgct gccgccaatg cacctttgca aattctgcat
    23041 ccgttggggc atttgcttta aatatatgaa tatcttgaaa aatgcactct ccggcggatc
    23101 ggtctgctca cccgcagaca gccgctgttg aaaccagtgc agcgctgctg cgcccaagaa
    23161 gatgacaaaa acaaaaaaaa ttgaaaaatt gcagaaaatt ttcttgcatg gtaaaatgaa
    23221 accatccaag tttgacagtt ttttcaggtg cgctttcaat gagtcagcag gaaaaaatta
    23281 ttctcttcgg gggctccttt gatccgatac accgcggaca tctcgaagtg gctgcccatg
    23341 cattgcatac gctcggcgcc gggcggctga tttttattcc cgcgcggcgc agtccgcaca
    23401 agcggcggat agccgccgaa ggcagtcatc gtctggcgat gattcggctg gccattgaag
    23461 gaacaaaccg cttggatgtc agcgactgtg agctgcgccg ccccgaaccc agctacacga
    23521 ttgatacggt tggcttcttc cggcagcagg cagggccgca ggcaaccctc tattggctga
    23581 tgggcgccga ccagctggaa gagtttggca agtggcatca agtcgggcgc ctgctggacc
    23641 tctgccgcat ctgcgtgatg ctgcgggggg gatatccatt gcccaacatc cgccggtttg
    23701 cgggcatctt taccgatgag cagattggcc gtctggaaaa cgacctcatc cgcacgcccg
    23761 aaattcacat cagcagcacc gacatccgcc ggcagctggc ctgcgggcat atgcccgccg
    23821 atgccctgca tccggctgtg gcagactata tcaaacagca ccgcctctat ggctataagc
    23881 cgtctgaatg agaacgccgg aaatatgcag aagtccggat aaagacttct gcccaaagaa
    23941 ataagcactg ctaacccgac agatattgtc cagatggtcc caattctgca tgcactatct
    24001 tgctatcttc aaaaatct
//
LOCUS       DAOTBO010000032        23930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1744_length_23930_cov_5.969885, whole genome shotgun sequence.
ACCESSION   DAOTBO010000032 DAOTBO010000000
VERSION     DAOTBO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 23930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23930
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1744_length_23930_cov_5.969885"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1876)
                     /locus_tag="PLE88_05620"
     CDS             complement(185..1876)
                     /locus_tag="PLE88_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM75976.1"
                     /translation="MTELEKIRVHELVIRMSDKDISPEEFSELDSLIASSPEAARYYN
                     ACMIASIGLHQSGKIIQSAQESIEPILEESLWRHLAESERTAAAVQIEKQQKPFEKAP
                     VDPMPYNLSQRNAAKRLLVFALTSIAALLCMMIYVYTHPRPIKEPVAVLVDQYNARWA
                     SNGQEMNPGLQLFNGQPLYLMQGVAKILFDYGAEVVIQGPAEVVLISPEKVSLQHGRL
                     YANVPRKATGFIVETPRSTVIDLGTEFGIKVDVTGAMDIHMFKGKANLVLGPKGRAED
                     NTILTAQQARRIDAETGEMIPIEVQPNSFIRDFNSQKGLVWRGDNINLADIVGGGNGF
                     GTGQIGYGIDPVTGLFVPFKVPNRPTSAGSGRFVPVHGNPFIDGIFVPDGGAGLVQVS
                     SQGHCFEDCPDTNGLYWIPALNGGQITDWEGGPMVSLLLQDKLYGDAACPAIFLHSNL
                     GITFDLEAIRQMLPAAQITEFRSVCGISQTGPNRVPYADFWVLVDGQLRFEQRGVKAA
                     QLYDITVPIRSNDRFLTLITTDGGEEFCLKDEHGAYAIDSDWALFGTPTLVLEAR"
     gene            complement(1873..2412)
                     /locus_tag="PLE88_05625"
     CDS             complement(1873..2412)
                     /locus_tag="PLE88_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM75977.1"
                     /translation="MDAGYNESERYKEFLRLLMANQHRIFGFIFALVPDMEAAEDLQQ
                     ETMLVMWEKYREFKPGTNFAAWGIQIARNKVYQYYHEKNRRKVVSFSDEALDNLIGNM
                     DVFDQTPERIKALQKCLSEISESDRKLLQMRYVNNQTFKQIAQEIQKPLPGLYKHMAK
                     VHYLLQRCVQRTLVAWNVI"
     gene            3209..7960
                     /locus_tag="PLE88_05630"
     CDS             3209..7960
                     /locus_tag="PLE88_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOM75978.1"
                     /translation="MKNSILPIFMILSSIAVIVPVLSADRRLIDFNDQWRFARGPQED
                     DAKQLTFDDGGWQAVTIPHDWAIAGPFNPQEHGYAAKLPWKGVGWYRKQFTLDAGDSG
                     KRVYLDFDGVMAFPKVYINGHLAGQWDYGYMSFRVDATPYIRFGQPNLIAVEVDTRSQ
                     GTRWYPGAGIYRKVTMTLCEPIHLTHWATFITTPEVTDQAAAVKVQTAVENHTDNNAS
                     IEISYLIIDPDGKTAAQTRQTAALASGETKSLEQVLRVDNPRRWDITAANLYTAAVMV
                     RSGGAITDSENIRFGIRTFAFTANDGFHLNGRRVQIKGVNLHHDQGPLGAAFYPRAME
                     RQLEIMKEIGVNAVRTSHNPPAPELLDLCDRMGLVVWDECFDKWDDKAGRVNGQPPLQ
                     EYGEKQIRNFVMRDRNHPCVVVWSIGNEINNASASREGKDGPRVSMMRQFVLKYDATR
                     PVGMADHIPQTANQPILDALDVVGWNYARRYSIFRERYPDKPIIYSESASALSTRGFY
                     ELPLPTTKTDYSDKLQVDSYDLNAAPWSDIADAEFALMEQDSFVAGEMVWTGFDYLGE
                     PTPFDAEARSSYFGIVDLCGIPKDRYYLYRSYWRPEAATVHILPHWNWPDRIGQVVPV
                     FVYTNGDSAELFLNGKSLGRRIKGQMPDKPVNLAEGKPASASSVQSDQYAAAMALDGS
                     SQTRWCAADGSSNQWLQADLQGIQTIRYISLEFEKEAKNYGYEIRVSSDGSSWQTIVH
                     QQTSNQPQWGGPREAFHQADVKARYVRIVFTALSSQTWASLRRLGVYERPVESDYYAP
                     TYRYRLRWNEVLYEPGELKAVAYKNGQEIGQAVMRTAGQPAHIRLTPDRTRLIADGMD
                     LSYVLVEAVDQDGTVCPLADNMVHFKIEGPAQIAGVGNGNPMSLEPFQADYRKLFYGK
                     AMLILRTVRGVSGPIRITAASEGLAGGQAELVAAADSPQQISRPIEISSPDGKLTAQF
                     KLQDGRPVYSIRLAEQLLLADSPLGLLCSSGDFTRQMQIAGVSDSVPAEQAYTLPHGK
                     RSRCLYQANQRTITLENQHRQPIEIIFSVSNDGVGFCYRLPQTVDRQQTSVLHEATGF
                     AFPEGTISWLHPMHDAKTGWEKTYPSYEAHYEIAQPVGKPAPYDAGWAFPALFCVGQA
                     GWVLVSETNVDQTYCACRLAKESAGGIYRIAFPQAAEHRGPQDPVEPQIQLPFTSPWR
                     IVIVGKDLRTIVESTLATDLAAPSKISDSAFIRPGRASWSWLRYDSGGTELGKLKEYL
                     DMAARLGWEYMLVDCDWDRKIGYEKIAQLAQEADAKGVKLILWYNSNGQWNTAPMTPK
                     DRMHLPAVRRQELRRLKEMGIAGIKVDFFGGDKQATMQLYMDILKDAADFGILCNFHG
                     ATLPRGWQRTWPNMITTEAVMGMEYCTFDQRNTDLQPQHCCVLPFTRNVVAPMDFTPV
                     VMSRSIRSVRRVTSPAFELALPVIFESGIQHFGLAPFEAAGLPEFAVDYLKAVPTAWD
                     ETRFVAGYPGRDVILARRKQTAWFAAGINGEKTTKQMAFDLSFLPAGQAAVLITDSPS
                     DDTLIMEKIDTAAKQFTIELKPCGGFVLYTL"
     gene            complement(7983..8330)
                     /locus_tag="PLE88_05635"
     CDS             complement(7983..8330)
                     /locus_tag="PLE88_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOM75979.1"
                     /translation="MAIPESQLETWAKQGAITTAKSTADSVKNALNSYNNWPNGIDFE
                     VYLQGSYKNDTNIRGDSDVDIVAQLNSTFYSNLSVLSIVVDYSLCRASILPCFCHYIY
                     LAGSRRVGLSPRG"
     gene            complement(8346..9263)
                     /locus_tag="PLE88_05640"
     CDS             complement(8346..9263)
                     /locus_tag="PLE88_05640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOM75980.1"
                     /translation="MTKDIEKFENKIILGDCLEIMKDIPDDSVDMTFADPPFNLKKKY
                     NHYEDSKEKEEYLQWCNKWISEMVRITKPSGSIFVHNIPRWLSHFAEHLNRIAFFRHW
                     ISWDSGGAPMGKTLLPNHYGILYYTKSKSYKNFKFYDIRYPHPRCRICKEFLKDYGGK
                     KDQAHDFGPLLSDVWSDIHRIRHKKRRDEHPCQLPVPLLERLILMSTDEGDIVFDPFV
                     GTGTTAVAAKRLGRKYIGIDIDPQYIKIAEKNLKQEKETKINGYYVSIYLGEIRTMRD
                     KDFKEIWKNRDEDVKFLENKMYRLNKKIY"
     gene            complement(9266..10264)
                     /locus_tag="PLE88_05645"
     CDS             complement(9266..10264)
                     /locus_tag="PLE88_05645"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:NF016443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HOM75981.1"
                     /translation="MTKIPKPTTESKFKLVINKNTFYLYDRDFEEKYEGYISSIRELL
                     LILRNKVSIEGLKKDFFVELIRDKENGLRALLALTGISNEYLKRLITLIRVIDDEELN
                     KLTLKSKWQTETEEISESGVSEWSDSKIAGLIKDNENFAKGIVNIFFEGSSVPVLTQA
                     LPLFELKKLSVEKLEFKIESLVDTLIRYKEKGSYAGKKENNPEVLIEKVLDELEIPYV
                     SGDLKKLVGVLDEEKRTMDFIIPNQENPLLIIESSYVVTTSSGQGDKAKTEINVARLI
                     KKYYPKALFIGFVDGIGWYVRKNDLKRMVGAYDDVFTFHKEELNRFKDLLSAIFGK"
     gene            complement(10266..11495)
                     /locus_tag="PLE88_05650"
     CDS             complement(10266..11495)
                     /locus_tag="PLE88_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75982.1"
                     /translation="MQNFKPITQKLFPNDYQLIDGTDEIFELNFALWEYENLNKENLI
                     KRSAYFKSIEGKPTIHYQTCNLQNLEEIHRNSSLRTKAFFLNGKYSTGYATHSLFPYR
                     GKFHPQMIRALLNMMDVKPGDLILDPMSGSATLSVEANLLGINSLAVDVSPFCCLMGR
                     VKTFALNLNSKKLNQITKEQEKIFEKLNKPKVPDYFVKNSNDESKPYYEIALLAYLDA
                     MGFARRSSKSLNTLFPVVLNRYMATIKNFQTAREKLDIKIGKSKIIEDSALNLPLEDD
                     EIDGVITSPPYSFAIDYVYNDKPQLEYLGYKPAKLKEDMIGLQGRGISEKLELYFSML
                     DKVFEELARVTKKGSKTIFIVGTNDIQTKGVRLETKIKELAEGQKFKFLFEILKPIKG
                     LQNTMKEEYILIFENNK"
     gene            complement(11498..11698)
                     /locus_tag="PLE88_05655"
     CDS             complement(11498..11698)
                     /locus_tag="PLE88_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM75983.1"
                     /translation="MSNKISENLKKLRAQKSYSLEKVARLADLSLNTVVKIENGINKN
                     PTIETITKLAKALDVEIDDLIK"
     gene            11782..12192
                     /locus_tag="PLE88_05660"
     CDS             11782..12192
                     /locus_tag="PLE88_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75984.1"
                     /translation="MEKVKQNYQGSSLLLPAHGRVGARETSTLVAHFAKQNTKFGSDR
                     KRGRAKIPSPQPPSFLPARAFQFCARSAQSFGIMLKKCSNFRIADRTLVLDFKKAFKI
                     AEKYHAEALCAETIPCDFTKSEKAERAGFEPAAG"
     gene            complement(12158..12244)
                     /locus_tag="PLE88_05665"
     tRNA            complement(12158..12244)
                     /locus_tag="PLE88_05665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12208..12210),aa:Ser,seq:gga)
     gene            complement(12463..14034)
                     /locus_tag="PLE88_05670"
     CDS             complement(12463..14034)
                     /locus_tag="PLE88_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5605 domain-containing protein"
                     /protein_id="HOM75985.1"
                     /translation="MKKRILKRMAVLYCVSAMAVAASAMRVEQWGIFELTLTGPSEGN
                     PFTEVQLSAVFRQGDRTFEPEGFYDGGGVYKVRFMPDRQGIWTYTTKSSCTVLDGKTG
                     TIEAAPPAANNHGPVRVANTFHFAYADGTPYRPFGTTCYAWTHQTEALEEQTLQTLAA
                     APFNKLRMCVFPKRYEWNQNEPRYYPFEGTPPDRWDFSRFNPEFFRHLEQRIGQLRDL
                     GIEADVILFHPYDKGHWGFDRMPAEADDRYLRYVVARLAAYRNIWWSMANEYDFMTEK
                     RPSDWDRFFQIVQSKDPYQHLRSIHNGTLLYSHCKPWVTHVSIQNGAAVADFGRAELY
                     RDVYNKPIVFDEVKYEGNIPNRWGRLTAEEMVLRFWMGLIAGTYVGHGETILDPNDVL
                     WWSKGGVLKGQSPPRLAFLRTIVQDGPADGFEPIDKWQDYPFAGKRGEYYLGYFGRQR
                     PTVWPFELHKAGLAEGMRFRVEVIDTWNMTITPVDGVFTVCKKDNYYFADTQARSVAL
                     PGRPYMAVRIRRVRD"
     gene            complement(14113..15639)
                     /locus_tag="PLE88_05675"
     CDS             complement(14113..15639)
                     /locus_tag="PLE88_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOM75986.1"
                     /translation="MLAKLFSVTLEGIDGILCEVEVDVSPAAMSKPTVVGLPDAAVKE
                     SIERVRSAIVNSGFKYPDLPYLVNLAPADVRKEGPAFDLPIALGVLACTGGLSSERLH
                     DFVVVGELALDGRIRPVNGMLSMAMTAAAGGYKGILVPIENAAEAAVVQDIEVYAVGS
                     LAQAVGFLSDLLPLEPTVIDIESLFHQASAYEVDFSDVKGQESVKRALTIAAAGGHNV
                     MMIGPPGAGKTMLAQRMATILPDMTLAESLETTRVYSSVGLLPKGKALIATRPVRAPH
                     HTASGPALVGGGAHPRPGELSLAHHGILFLDEFAEFPRTVLEMIRQPLEDGTVTISRA
                     KGTLHFPARFVMLAAMNPCPCGYFGTNAKRCRCTPNQVERYLAKVSGPLLDRMDIHIE
                     VPPVEFHKLRSTAAGSSSKELRRQVQEARQRQLARFGKSTQTNATMTHKQVETFCSLD
                     PQAEMMLKQAITEFHLSARAHDKICKVARTIADLEQQEQIQPHHLAEAVSYRKLDRKL
                     "
     gene            15843..16379
                     /locus_tag="PLE88_05680"
     CDS             15843..16379
                     /locus_tag="PLE88_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM75987.1"
                     /translation="MDMIEYLRKRRSVRAFDNRPIEPDKIALLKEAVLRSPSSRNFDP
                     WEFIFVDDKQLLEQLSRSKPHGAEFLKDAAVGIVVCADPAVSDVWIEDCAIASILAQL
                     TAQSLGLGSCWIQIRQRFYNSRMSSETYIRNVLGIPEALCVLCIIAVGYPAEYPQPIP
                     RRQLKDAKIHVNRMQSAR"
     gene            complement(16349..17191)
                     /locus_tag="PLE88_05685"
     CDS             complement(16349..17191)
                     /locus_tag="PLE88_05685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM75988.1"
                     /translation="MDSFFVSDLDGTLLRSDGTLSDYSRKTLTELLQAGLQFTVASAR
                     AWGEIVPVLGDLPLTLPVIAVNGAFLTDYKTGRHLVINSIARDFAESIYHHILDHQLE
                     PFIVTHNGTEDCLYWQNLKNEQMQWYHDMLHVQKDKRIRRISDLRHALAEQVIAFAVM
                     GPPDQVKALAEYLDAQYPALLENFLFENFYTPGHWWLTIHDQKACKSKAVSMLARMTG
                     HTLDSITVFGDHINDIKMFQTAGRAVAVANAEDQLKRCADLVIGSNDEDAVVRYLADC
                     MRLT"
     gene            complement(17281..17631)
                     /locus_tag="PLE88_05690"
     CDS             complement(17281..17631)
                     /locus_tag="PLE88_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM75989.1"
                     /translation="MIIKKMTDVRLQDVTMDGAENVKVRVLFGPADQVPTFAMRLFEL
                     GPGGHTPFHSHPFEHEVVVLDGDIAAVDESGPHPVQMGQVLLVMPNEKHQFINRSGTH
                     PARFLCLVPAAYQK"
     gene            complement(17770..20001)
                     /locus_tag="PLE88_05695"
     CDS             complement(17770..20001)
                     /locus_tag="PLE88_05695"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOM75990.1"
                     /translation="MPSNPQVVARQVERIVRQLHSLSTFPSVAADLLGRLNDSAAGPE
                     ALAEHIQADPALAAMVLSLAHQEGVRFSGGPSVAEAAAKLPAALLREALISVKVFDVL
                     EGGESPDAQRPLPRREMALHALAAGCCADELAQCVLPEEQRTAAYLAGLLHDIGKYAL
                     DEVMPKSFARMVEQARSGSCCLIEIEQQHLGLDHALIGKRLAEKWFLPDAITAAIWLH
                     HCDAQTLAADLPYGPIVRVAALADRLVRTAGIGHSGSYDTPQDIAELAQLLSLNSEQI
                     SRIADSLAETVSRRSRSLGLGGAAGSDRQYVSLIRKTAVHLAESNRKLAESTMEHRLL
                     TAQAKLIGDFLDAIDQNALPADIAEAFAAAWQTHCQSGLTCVYVVPDATEPFVEAAVT
                     DRQGRTDIKLLQMPDGIPPIPHSIQHQSAIQPAADAAKWILEQLECDFDPARMKMVSL
                     SIGDQIPAVLVYEDFSPLREAARDSMVLLCCRIAAAAIAMAQAVRKNGEMAERFVQMM
                     GTLRQARSELTRRQSLEGLAQMAAGAAHELNTPLAVISGRAQLLLTAEEDPDKKQMLR
                     QIQQRTEEISQIISDLMAFAQPAAPQKRTVPIDELLRKAIDKTRMEAGLSALEITISG
                     AEQAPPVYVDAHQVIQSLSYILMNALQSYKGGSGPIWIDCLAGSDERSAVVAIRDAGC
                     GMDADTLAKAVQPFFSYRPAGRRRGMGLAHAQRLLLLNGGRLSLDSKPDKGTTVTIVL
                     PKV"
     gene            complement(20036..20638)
                     /locus_tag="PLE88_05700"
     CDS             complement(20036..20638)
                     /locus_tag="PLE88_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM75991.1"
                     /translation="MEKIKNLFTTGEAAQICNISQQTIIRCFDSGRLGGFRVPGSRFR
                     RIPRDQLIKFMRENSIPLDNLNAQKTGKVKVLIVDDDTEIVELMVDVLSRDGRFEIRT
                     ASSGYDAGMQTQQFMPDVIILDYMLPDVNGNIVCQTIKRNPDFENIKIIIVSGVVNQD
                     EIQALLNAGAAEFLKKPFNIAELVDKILSVVDQSNLSSVS"
     gene            complement(20939..21397)
                     /locus_tag="PLE88_05705"
     CDS             complement(20939..21397)
                     /locus_tag="PLE88_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOM75992.1"
                     /translation="MEFKSIHEVLQFALSKEEASVQFYRELAGRMQNPASRRLAESLA
                     QQETSHVQMIALEMNKQGFCVRANEQLEDKEFIWDEHLQLDDEARNMNPVEIMAMAIQ
                     KERAAFQLYAQLLGLAHDIQFRTMLMDLAEEEVRHILLLEQQYEILIHEK"
     gene            complement(21411..21995)
                     /locus_tag="PLE88_05710"
     CDS             complement(21411..21995)
                     /locus_tag="PLE88_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HOM75993.1"
                     /translation="MMTDNHFIDAAADGASSETRVLRPEMRHQDIVFRGTSLTAEERN
                     KIRKALRTLHTLEIMAVAVYRCQIGRQNTELNRELIAAMCNEMTHVQDFQVGLYEYGL
                     SPSLFRWAWHLAGCVMGTIARLLGAEAILNMGIWVESKAVSHYSRLLEAAPWDEPMRN
                     IIRKDQEDERGHIRIWRRLLTAAQQKQPTALQRP"
     gene            22291..22569
                     /locus_tag="PLE88_05715"
     CDS             22291..22569
                     /locus_tag="PLE88_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75994.1"
                     /translation="MTWWEAEQIANYLEKLEEALEDWSLTISQMRDELSELSRHIKKI
                     QAELPRTWQASERLFLTQLSERAAALQEHIRERLKREKPRDSRHKISY"
     gene            22631..23266
                     /locus_tag="PLE88_05720"
     CDS             22631..23266
                     /locus_tag="PLE88_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM75995.1"
                     /translation="MHIDTLVMGDFQTNCYCVRPDASAGSCLVIDPGLEPEPLVQFLT
                     NKTIKVEAIILTHGHADHIGGVETLRQYWPDVQVAIHKDDASMLTDPADNLSILAGYM
                     VQARPAEVLLDSEDVYYQAAGLRFKILHTPGHTPGGICLYASEDGVLFSGDTLFAGSV
                     GRTDFPGGNYALLIQMITQKLLVLPDQTRIYPGHGPATTLRNEKKFNPFLI"
     gene            23368..23625
                     /locus_tag="PLE88_05725"
     CDS             23368..23625
                     /locus_tag="PLE88_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOM75996.1"
                     /translation="MCDSCGCGEKTFEAKVAEVIDSIRPMLQNDGGDIELVGIDADKT
                     VRVRLQGACRGCPGAQMTLKMGVERLLKQRVSEVKQVVAVS"
     gene            complement(23660..>23930)
                     /locus_tag="PLE88_05730"
     CDS             complement(23660..>23930)
                     /locus_tag="PLE88_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75997.1"
                     /translation="LSVLAKENKIPFYIAAPTSTIDLTCRTGADIPVEHRDGQEVRQN
                     TAPQDIQVYNPAFDITPSANITAIITERGILDPINPEAIFRHIVP"
BASE COUNT         5952 a         6204 c         5666 g         6108 t
ORIGIN      
        1 gccaacatca caacatactt cttcatcttt ccttacctcc aaaaaagtta aagttgcatt
       61 agtacatcaa ttttttacat cactcttaat atttccaaac gctgttttct tctcttcact
      121 tttcctcttt ttgccgctgt ttgtctcctt tcttaacttt actgaactat ttactgtatt
      181 ttcgttatcg ggcttccaaa accaatgtcg gtgttccaaa taacgcccaa tcactgtcga
      241 tagcataggc gccgtgctca tccttcagac agaactcttc tccgccgtca gtggtgataa
      301 gagttaaaaa gcggtcattc gaacgaatcg gcaccgttat gtcatataat tgggctgcct
      361 tgactcctcg ttgttcgaac cgcagctgtc catcgaccaa tacccaaaaa tctgcataag
      421 gaacgcgatt gggaccggtc tgactgatgc cacagacaga tcgaaattct gtaatttggg
      481 ctgccggcag catttgacga atggcctcaa ggtcaaatgt aatgccaaga ttggaatgaa
      541 gaaaaatagc cgggcaggcc gcatcgccat acaacttgtc ctgcagcagc aaagaaacca
      601 taggcccccc ctcccaatcg gttatttggc cgccgtttaa ggcggggatc caatacagcc
      661 cgttggtatc cggacaatcc tcaaagcaat ggccctgtga ggatacctgt acaaggcctg
      721 caccgccgtc ggggacaaaa ataccatcaa taaatggatt accatgaacc ggaacaaatc
      781 tgccgctgcc ggcacttgtg ggtctattgg gcaccttgaa tgggacaaac agccccgtta
      841 ccggatcaat accgtaacca atctgaccgg ttccaaagcc gtttccgccg ccgacaatat
      901 cggccagatt gatattatcg ccgcgccaga caagcccttt ctgggagtta aaatcccgaa
      961 tgaagctgtt cggctgaacc tcaatgggta tcatttcacc cgtttcggca tcaatccgcc
     1021 gggcctgctg tgccgtcaga atagtattgt cctctgcacg gcccttggga cccaacacca
     1081 gattagcctt ccccttgaac atatggatat ccattgcgcc ggttacatcc actttgatgc
     1141 caaattctgt tccgaggtca ataaccgtgg agcggggtgt ttctacaata aagccggttg
     1201 ctttacgggg cacattggca tacagccggc catgctggag agatactttt tccggactta
     1261 tcagcacgac ttcggcaggc ccctggatga caacttcagc cccataatca aaaaggattt
     1321 tggcaacacc ctgcatcaga tacagcggct gaccattgaa aagctgcagc ccgggattca
     1381 tttcctgacc atttgatgcc cagcgggcat tatactgatc aaccagcaca gccaccggtt
     1441 ctttgatggg gcgggggtgg gtatagacat aaatcatcat gcacagcagc gccgctatgg
     1501 aggtcagtgc aaagaccaag agccgcttgg ccgcatttcg ctgcgataaa ttgtacggca
     1561 tgggatctac gggtgctttt tcaaagggct tttgctgctt ttcaatctgc actgccgcgg
     1621 ctgtacgttc cgattcggcc agatgacgcc acagggattc ttcgagaatc ggctctatgc
     1681 tttcctgagc cgactgaata attttaccgg attgatgcag cccgatcgag gcaatcatac
     1741 atgcattata gtaccgtgcc gcttccgggg atgaagcaat cagactgtcc agctctgaaa
     1801 actcttccgg cgaaatgtct ttatccgaca tccggattac caattcatga acgcggattt
     1861 tttcaagttc ggtcatatca cattccatgc caccaaggtt ctctgaacac accgctggag
     1921 gagatagtga actttggcca tatgcttgta gagtccggga agcggttttt gtatctcctg
     1981 agcaatctgc ttaaatgttt gattgttcac ataccgcatc tgcagcagct tgcggtccga
     2041 ttcgctgatt tcggacaagc atttctgcag cgctttgatg cgctccgggg tctggtcaaa
     2101 gacatccata ttccctatca ggttatccag cgcttcatcg ctgaaagaaa ccaccttgcg
     2161 ccggtttttt tcatgataat actgatacac tttgttgcgg gctatctgga tgccccacgc
     2221 cgcaaaattc gttccgggct taaactcccg gtatttttcc cacatcacaa gcatcgtctc
     2281 ctgctgcagg tcttcagccg cctccatatc agggacaagg gcaaaaataa atccgaatat
     2341 gcgatgctga tttgccatca gcaaccgcag aaactcctta tagcgttcag attcattgta
     2401 gccggcgtcc actaagattg tctcctcata cacctttttc gggcgtttct acaccctgcc
     2461 aacacatatt cttctttttt ggtaatcaag caaatagagg gaattagaat aaaaagtagg
     2521 ttttttttgt aataatttcg gccacattca tgacttctca ggtctgttat ttgtcgtctt
     2581 gataacactc gccagcaaag gcattatttt cagtattatt ttctcaagtt tctcacataa
     2641 gacgacaata tagagtcgta tacctaaaat atctgtattg cataatgctc tataggaaaa
     2701 ttgttaaaaa tgctaaaatc tttcgattat ggctatcttt tcctacaatg ctcccttttg
     2761 ttcaggtgcg ggatgacagc ttccgattat ggctgagcac cgatggctcg cccgatccat
     2821 gcggtcctta ttgcacaggc tgccggctgg acgaacccgc gtgaggggac caaatatcca
     2881 tccaagcagg ccgacagcag tctattgagt aaagaaagca tatcagccga ttttcggcat
     2941 agtgccgcac ctgcagccgg cagtatcctt cagatggaac tgtcagccgc gtttttttgt
     3001 agctgactcg tgcaagaaaa cggttccttt tatgtttttt tatcaacaag cacagcgccg
     3061 tcctgaaata gccataaaat acgacttgat ttatcgcctc tgaccgatag aatggcctct
     3121 gatgattcag aagaaactgt tcgcccaatg ggttcaaggt cggctgtttg gagtgcctct
     3181 gaaaacacta ttttacagga caatcgttat gaaaaattca atccttccaa tctttatgat
     3241 tctgtcatct attgctgtta tcgtccctgt gctttcggcc gaccgccggc taattgattt
     3301 taatgaccaa tggcgctttg caagaggccc gcaggaggat gatgccaaac agctgacatt
     3361 tgatgacggc ggctggcagg cggtaaccat tcctcatgat tgggcgattg caggtccgtt
     3421 taatccgcaa gagcacggat atgccgccaa actgccgtgg aaaggcgtcg gctggtaccg
     3481 caaacaattc accctcgacg ccggcgacag cggcaagcga gtctatctgg actttgacgg
     3541 cgtgatggct ttccccaaag tgtacatcaa cggccatctg gcaggccaat gggactacgg
     3601 atatatgtcc ttccgggtag atgccacgcc gtatatcagg ttcggtcagc ccaatctcat
     3661 cgctgtcgaa gtcgataccc gcagccaagg tacgcgatgg taccccggcg ctggcattta
     3721 ccgcaaggta acaatgacgc tttgcgagcc gattcatctg acgcactggg caaccttcat
     3781 taccacgccg gaagtaaccg atcaggccgc cgctgtaaaa gtccagacag ccgttgagaa
     3841 ccatacggac aacaatgcgt ctattgaaat ttcttatctt ataatagacc cggatggaaa
     3901 aacagccgcc cagacacggc aaacagcagc gctggcttcc ggcgaaacga aaagtcttga
     3961 gcaggttctt cgggtggaca acccgcgccg ctgggatatc accgccgcca atctctatac
     4021 ggcagccgtc atggtccggt caggcggcgc aataaccgac agcgagaaca tccgcttcgg
     4081 catccgcacc tttgcattta cggccaatga cggttttcat ctcaacggcc ggcgcgtgca
     4141 gataaaaggc gtcaatctgc atcatgatca gggcccgctg ggggcagcgt tttatccgcg
     4201 ggcgatggag cggcagctgg aaatcatgaa agaaatcggc gtcaatgccg ttcgcaccag
     4261 ccataatccg ccggcgccgg aactgctgga tttgtgcgac cggatgggat tggtcgtgtg
     4321 ggatgaatgc tttgacaaat gggatgacaa agccggccga gtcaatggac agcccccgct
     4381 gcaggaatac ggggaaaagc agattcgcaa tttcgtaatg cgggaccgca atcatccttg
     4441 tgtggttgtc tggtctatcg gcaacgaaat caacaatgcc tccgccagcc gggaagggaa
     4501 agacggcccg cgggtcagca tgatgcgcca gtttgttctt aaatacgatg ccacccgccc
     4561 tgtcggcatg gcagaccata ttccgcaaac ggccaatcag ccgattttgg atgctctgga
     4621 tgtagtcggg tggaactatg cccggcggta ttccattttc agagaacggt atccggacaa
     4681 gccgattatc tacagtgaat cggcatcagc cctgagcacg cgcggttttt atgaattgcc
     4741 gctgccgact accaagaccg actattccga taagctgcag gtggactcct atgacctcaa
     4801 tgccgctcca tggtcggaca tcgccgatgc ggaatttgcc ctaatggaac aagacagttt
     4861 tgtcgcaggc gaaatggtct ggacaggatt cgactatctg ggagaaccca ccccctttga
     4921 tgcagaggcg cgaagctcct acttcggaat tgtggacttg tgcggcattc ccaaagaccg
     4981 ctattacctg taccgcagct attggcggcc ggaagcagcg accgtccaca ttctgccgca
     5041 ttggaactgg cccgatcgca tcggacaagt agttccggta tttgtttaca ccaacggaga
     5101 ctccgcggag ctgtttttaa acggaaaatc gctgggacgc cgcatcaaag ggcagatgcc
     5161 ggacaaaccc gtcaatttgg ccgaaggcaa gccggcctcg gcttcgtctg ttcagtcaga
     5221 ccaatacgct gctgcaatgg ccctcgacgg cagcagccaa acacgctggt gtgccgccga
     5281 tggaagcagc aatcaatggc tgcaggcgga tcttcagggc atccagacga ttcggtacat
     5341 tagtcttgaa tttgaaaagg aagccaaaaa ttacggctat gaaatccgtg tttcgtccga
     5401 cggcagcagc tggcagacga ttgttcatca gcagaccagc aatcagcccc aatggggcgg
     5461 cccgcgcgag gcctttcacc aagccgatgt aaaagcccgt tatgtccgca ttgtatttac
     5521 tgctctcagc agccagacat gggcatccct tcgccggctg ggagtctatg agcgtccggt
     5581 tgagtcggat tattatgccc cgacctaccg gtatcggctg cggtggaatg aggtcctgta
     5641 cgaaccgggt gagctgaaag ccgtggctta caaaaacgga caagaaatcg gccaagctgt
     5701 gatgcggacg gccggacagc cggctcacat tcgcctgaca cccgaccgga cccggcttat
     5761 cgccgacggg atggatttat cttatgtttt agtcgaggca gttgatcaag acggtaccgt
     5821 gtgtcctttg gcagacaata tggttcattt caaaattgaa ggccccgccc aaattgccgg
     5881 cgtcggcaac gggaatccga tgtctctgga gccgtttcag gccgattacc gcaagctctt
     5941 ttacggcaaa gcaatgttga ttctgcgcac ggtcagagga gtatccggtc cgattcgcat
     6001 tacggcggca tcagaaggtt tagccggcgg tcaggccgaa ttggtcgccg ccgccgactc
     6061 tccgcagcag atcagccggc cgattgaaat ttccagcccc gacggcaagc tgacagccca
     6121 atttaagctg caggatggac ggccggtgta cagcatccgt ctggcagagc agctactttt
     6181 ggccgattcc ccgctgggtc tgctctgcag cagcggagat ttcacccgcc agatgcagat
     6241 agcgggggtc tccgatagcg tgccggctga acaggcttac acgctgccgc acggcaaacg
     6301 cagccgctgc ctgtatcagg ccaatcagcg gacaatcacg ctggaaaacc agcaccgcca
     6361 gcccatagaa atcatcttct cggtctccaa tgacggcgtg gggttctgct accggctgcc
     6421 gcaaactgtt gaccggcagc agacctctgt attgcacgaa gcaaccggct ttgcttttcc
     6481 ggagggaacc atcagttggc tgcatccgat gcacgatgcc aaaaccggct gggagaagac
     6541 gtatccgtct tacgaagcgc actatgaaat tgcccagccg gtcggaaagc cggcgccgta
     6601 tgatgcgggc tgggcatttc cggcgctgtt ttgtgtggga caggccggct gggtgctggt
     6661 cagtgaaacc aatgtggacc agacttattg tgcctgccgt ctggcaaaag aatccgccgg
     6721 cgggatctat cgcattgcct ttccgcaggc ggccgaacat cgtgggcctc aggatccggt
     6781 tgagccgcag attcagctgc ctttcacaag cccgtggcga atcgtgattg tcggcaaaga
     6841 tctcaggacg attgtggaat ccacgctggc aaccgacttg gccgcccctt ccaaaatcag
     6901 cgattcggcg ttcatcaggc ccgggcgggc ttcgtggagc tggctgcggt acgacagcgg
     6961 cggcaccgag cttggcaagc tgaaggagta tctggatatg gccgctcggc tcggctggga
     7021 atatatgctg gtagactgcg actgggaccg caaaatcggc tatgaaaaaa tcgcccagct
     7081 ggcccaagaa gccgatgcca aaggagttaa actgatcttg tggtacaact ccaacggcca
     7141 gtggaatacc gccccgatga cgcccaaaga tcggatgcat ctgcccgctg tccggcgcca
     7201 agaactcagg cggctcaaag aaatgggcat tgccggcatc aaagtggatt tcttcggcgg
     7261 cgataaacaa gcaaccatgc agctgtatat ggatatcctc aaggatgcgg ccgattttgg
     7321 aatcctgtgc aatttccacg gcgcaaccct gccccgcggc tggcagcgga catggcccaa
     7381 tatgattacc accgaagcgg taatggggat ggaatactgc acttttgacc agcgaaatac
     7441 cgacctgcag ccccagcact gctgcgtgct gccctttacg cgcaatgtcg tagcgccaat
     7501 ggatttcacg ccggtggtga tgagccgcag catccgctcc gtccgccggg ttaccagtcc
     7561 ggcattcgag ctggcgctgc cggtcatttt tgaatccggc atccagcatt tcggactggc
     7621 gccctttgag gcggccggcc tgccggagtt tgctgtcgac tacctgaaag ccgtgccaac
     7681 ggcatgggat gaaacccggt ttgttgcggg ttatcccggc agagatgtca tactggcacg
     7741 gcggaagcaa acagcatggt ttgcagccgg catcaacggg gagaaaacga ctaaacagat
     7801 ggcctttgac ctgtcgtttc tgccggccgg tcaagcggca gtattgatta ccgacagccc
     7861 ctcagatgat acgcttataa tggagaaaat cgatacagca gcaaaacaat ttacgattga
     7921 attgaaaccc tgcggcggat tcgtccttta tacgctttaa ttgtctcttt ttaattgtct
     7981 ctttagcccc gcggagataa gcccaccctt cggctcccgg caagatagat ataatggcaa
     8041 aaacagggca agatagaagc tctacagaga gagtaatcaa cgactatcga caagacggaa
     8101 agattactat aaaaagttga gtttagttgg gcgacaatat caacatcgct gtcgccacga
     8161 atgttagtat cgtttttata tgaaccctgc aaataaacct caaaatcaat tccattcggc
     8221 caattattat aagaatttaa agcattttta acagaatcag cggttgattt agcggtagta
     8281 attgcacctt gttttgccca tgtttctaat tgtgattctg gtatagccat agcttttgcg
     8341 tatatttaat aaattttttt gttcagtctg tacattttat tttccaaaaa ttttacgtct
     8401 tcatctctgt ttttccaaat ctctttaaaa tctttgtctc tcattgttct aatctcgcca
     8461 aggtagatgg aaacataata cccatttatc tttgtttctt tttcttgttt gagatttttc
     8521 tcagcaattt ttatatactg cgggtcaatg tctatcccga tatatttccg tcctaaacgt
     8581 ttagccgcga cggctgttgt ccccgttcca acgaaagggt caaatacaat atcaccttca
     8641 tcagtgctca tcaaaatcaa tctttccaaa agtggtacgg gcaattgaca ggggtgttcg
     8701 tctctccgtt tcttgtggcg tattcggtgt atatcagacc aaacatcaga aagaagcgga
     8761 ccaaagtcat gtgcttggtc ttttttgcct ccataatctt tcaaaaattc cttgcaaatc
     8821 cgacaccgtg ggtgcggata tctgatatcg taaaatttaa agtttttata tgattttgat
     8881 tttgtgtaat acaaaattcc gtaatggttt ggaagtaatg tttttcccat tggcgcaccg
     8941 ccggaatccc aagaaatcca atgtctaaaa aaagcaatcc tgtttagatg ttctgcaaaa
     9001 tgagaaagcc agcgagggat attatggaca aaaattgatc cacttggttt tgttattctt
     9061 accatttcgc taatccactt attgcaccat tgcagatatt cctctttttc tttactgtcc
     9121 tcgtaatgat tatatttttt ctttaggtta aagggtgggt cagcaaaagt catatccact
     9181 gaatcatcgg ggatatcctt cataatttct aaacaatcac ctaaaattat tttattctca
     9241 aatttttcaa tatcttttgt cataattatt taccgaaaat agctgatagc aaatctttaa
     9301 aacgatttaa ttcttctttg tgaaaagtaa aaacatcgtc gtatgctccg accattcttt
     9361 ttaggtcatt tttcctcaca taccagccaa taccatctac aaagccgata aataatgcct
     9421 tgggatagta ttttttgata agtcgtgcaa cattgatttc tgtttttgct ttatcgccct
     9481 gccctgaaga agtggttaca acataagaag attcaataat aagcaacgga ttttcttggt
     9541 tgggaataat aaaatccatt gttctctttt cttcatcaag tacaccaact aattttttca
     9601 aatctccgga aacataagga atttcaagtt catctaaaac tttttcaata agcacttctg
     9661 gattattttc ttttttccca gcatacgatc ccttttcttt gtaacgaatt aaagtatcaa
     9721 ccaaagactc tattttaaat tctaatttct caacgcttaa ctttttcagc tcaaataagg
     9781 gtagtgcttg agtaagcact ggcacacttg agccttcaaa gaaaatattt acaataccct
     9841 ttgcaaaatt ctcattgtcc ttaataaggc cggcaatttt tgagtcgctc cactcactaa
     9901 ctccgctttc gctaatttct tctgtctccg tttgccattt actttttagg gtaagtttat
     9961 ttaattcttc atcatcaata accctgatca aggttatcag ccgttttaaa tattcgttcg
    10021 agataccagt aagggctaat aatgctcgca aaccgttttc cttatcacga attaactcca
    10081 caaaaaaatc ttttttaagc ccttctattg agactttatt tcgtaaaatt aaaagcaatt
    10141 ctctaattga ggaaatatag ccttcatatt tttcctcaaa atcccggtca taaagataaa
    10201 aagtattttt atttataacc agtttaaatt ttgattcagt tgttggtttt ggaattttag
    10261 tcatattatt tgttgttttc gaaaattaaa atgtattctt ctttcatcgt gttttgaagc
    10321 cctttaattg gctttaaaat ttcaaaaaga aatttaaact tttgtccttc tgctaattct
    10381 ttaatttttg tttccagtct cactcctttt gtctgaatat cgttagttcc aacaataaag
    10441 atggttttac ttcctttttt ggtaaccctt gctaactcct caaatacttt atccaacata
    10501 ctaaaataaa gttctaattt ttcgctaatt cctctgcctt gcaacccaat catatcttct
    10561 tttaatttag ctggtttgta cccaagatat tccaattgtg gcttatcatt atatacataa
    10621 tcaattgcga aagaataggg tggagaggta ataacgccat caatctcatc atcttctaat
    10681 ggcaaattta atgcgctatc ctcaataatt ttactctttc ctatttttat gtctaatttt
    10741 tctcttgcgg tctgaaaatt tttaatagta gccatatatc tgttaagtac aaccggaaaa
    10801 agagtattta gagatttaga acttctacga gcaaacccca tagcgtcaag ataagctaac
    10861 aaggcgattt cgtaatacgg cttgctttcg tcattgctat tttttacaaa atagtcaggc
    10921 acttttggtt tattcaattt ttcaaaaatt ttctcttgct ctttggttat ctgattaagt
    10981 tttttactat taagattcaa tgcaaaagtt tttactcgtc ccattaaaca acagaatgga
    11041 ctcacatcca cagcaagcga attaataccg agtaaatttg cttctactga taaagtcgca
    11101 ctaccagaca ttgggtcaag aataaggtcg ccgggcttaa catccatcat atttagtaat
    11161 gccctaatca tctgcggatg aaatttccct ctatagggga ataaactatg tgtagcatat
    11221 cctgtagaat atttgccatt tagaaaaaat gctttggtgc gtaatgaact attgcgatgt
    11281 atttcttcta aattttgaag attacaggtt tgatagtgaa tagttggctt gccttctatg
    11341 gatttaaaat aagcactgcg cttgattaaa ttttctttat taagattttc gtattcccaa
    11401 agagcaaaat ttaactcgaa gatttcgtca gttccatcga ttaactgata atcattaggg
    11461 aataattttt gggtaattgg tttaaaattt tgcataatta tttaatcaaa tcgtcaatct
    11521 ctacatctaa cgctttcgca agtttggtta tggtttcaat ggtagggttt ttatttatcc
    11581 cattttcaat ttttaccaca gtatttaaag agaggtcagc gagacgagcc actttttcaa
    11641 gagaataact tttttgagcc cttagtttct ttagattttc tgaaatttta tttgacattt
    11701 gcatagttat tgtttaacat tatagtatat agattatatt ctaaacattc ttctttttct
    11761 attataatga taactaatta tttggaaaaa gtaaagcaaa attatcaagg gagttccttg
    11821 ttgttgcccg cgcacgggag ggttggggca agggaaacat caactctggt ggcgcatttt
    11881 gcaaagcaaa atacgaaatt cggtagcgac agaaagcgag ggcgggcaaa aattccttcc
    11941 ccccaacccc cttccttttt gcccgcccga gcgtttcagt tttgcgcgcg aagcgcacaa
    12001 tcattcggaa ttatgctcaa aaaatgttcg aacttccgca ttgccgaccg cactttggtt
    12061 ttggatttca aaaaagcctt caaaattgcg gaaaaatacc atgccgaggc actttgcgcc
    12121 gagacaattc cttgcgattt cacaaaaagt gaaaaagcgg agagggcggg attcgaaccc
    12181 gcggcagggt aaaccctgca cagcctttcc aaggctgctc gataagccgc tccgacacct
    12241 ctccggctgc aattctgcca caatctgttt tagcagatat ttttaatccc ttttgtcacc
    12301 ggcagttata tatttccccg gcaatttaat ccgtcaacag gcagtatctt atcaaactgc
    12361 cgcggcaaaa tcaaagcaaa atcataaaac tatcgaccct gcttgggctt attctttgac
    12421 ctgttgtttt aattgttctg tgctgcacaa tacatcttcc agtcagtccc gaacccgcct
    12481 gatccggacg gccatatacg gtctgccggg cagcgcaacc gagcgggctt gcgtgtcagc
    12541 gaaataatag ttgtcctttt tgcaaaccgt aaaaacccca tcaaccggag taatggtcat
    12601 attccatgtg tcaatcacct caacccgaaa ccgcatgccc tccgccagac cggccttgtg
    12661 aagttcaaac ggccagacag tcggcctctg gcgtccaaaa taccccagat agtattcccc
    12721 gcgtttgcct gcaaaggggt aatcctgcca cttgtcaatc ggctcaaagc catcggccgg
    12781 accgtcttgg acaatcgtcc gcagaaaagc cagccgcggc ggactttgcc ctttcagaac
    12841 accgcctttg gaccaccaca ggacatcgtt ggggtcaagg atggtctcgc cgtgccctac
    12901 ataggtgcct gcaattagac ccatccagaa ccgcagcacc atttcctctg ccgtcaaccg
    12961 gccccagcga ttgggaatgt tgccttcata tttgacctcg tcaaaaacaa tgggcttgtt
    13021 gtacacatcg cggtacagtt cggccctgcc gaaatccgca accgctgcgc cgttttggat
    13081 gctgacgtgt gtcacccacg gcttgcaatg gctgtacaga agtgtgccgt tatgaatcga
    13141 acgcaggtgc tgatagggat ctttggactg gacgatttga aaaaaccggt cccagtcgga
    13201 ggggcgtttt tccgtcataa aatcgtattc gttggccatc gaccaccaga tattccggta
    13261 ggccgctaag cgtgccacca cataccgcaa atagcggtca tctgcttccg ccggcatgcg
    13321 gtcaaatccc caatgcccct tgtcgtaggg gtgaaacaag ataacatccg cctcaatccc
    13381 cagatcgcgc agctggccga tgcgctgttc caaatgcctg aagaattccg gattaaaccg
    13441 tgaaaaatcc caccggtcag gaggagtccc ctcaaaggga taataccgcg gctcgttctg
    13501 attccactcg tagcgtttgg gaaacacgca catgcgaagt ttgttaaacg gcgctgcggc
    13561 cagtgtctgg agtgtttgtt cttccagcgc ctcggtttga tgggtccagg cataacaggt
    13621 cgtaccgaac ggccggtagg gcgtaccgtc ggcatacgcg aaatgaaagg tattggcgac
    13681 gcgcaccggc ccgtgattat tcgctgccgg cggggctgcc tctatggtac cggttttacc
    13741 atccagtacc gtacagctgc ttttagtcgt ataggtccaa atcccctgcc gatccggcat
    13801 gaaccgcacc ttgtacacgc cgccaccgtc atagaatccc tccggctcaa atgtgcggtc
    13861 tccctgcctg aagacggcgc tgagctgaac ttctgtaaac ggattgccct ccgacggacc
    13921 tgtcaaggtc agctcaaaaa tcccccattg ttcaacacgc attgcgctgg cagccacggc
    13981 cattgccgaa acgcaataca ataccgccat ccttttgaga atgcgtttct tcatatactg
    14041 gttcctttcg aaaaaggggg ggcagatacg ttctgtcgtc gggcagatgg tatgtgtccc
    14101 cgtcttctgc tgtcaaagtt tccggtcgag tttgcggtag ctgaccgctt ctgccagatg
    14161 atgcggctgg atttgctcct gctgttcaag gtcggcaatg gtccgagcaa ccttgcagat
    14221 tttatcatga gcacgggcgg acaagtgaaa ctcggtaatg gcctgcttga gcatcatttc
    14281 ggcctgcggg tccagactgc agaaagtctc aacctgtttg tgggtcattg tggcgttggt
    14341 ctgggtgctt tttccaaagc gtgccaattg tctctgccgg gcttcctgca cctgccggcg
    14401 cagctcctta gagcttgagc cggctgccgt gctgcgaagc ttgtgaaact ccaccggcgg
    14461 cacttcgata tgaatatcca tccggtccag cagcggcccg gacactttgg ccagatagcg
    14521 ttccacctga ttgggcgtgc agcggcagcg tttggcattg gttccaaaat agccgcacgg
    14581 gcagggattc atcgctgcca gcatgacaaa ccgggccgga aagtgaagcg tacctttggc
    14641 acgggaaatg gtaactgtac cgtcttccag cggctggcga atcatttcca gcacggtgcg
    14701 gggaaactcg gcaaactcat ccagaaacaa gatgccatgg tgagccaagc ttaactcgcc
    14761 cggtcgggga tgcgcgccgc cgccgaccag cgccggcccg gacgccgtat gatgcggggc
    14821 ccgaaccggc cgggttgcaa tgagagcttt gcctttgggc agaaggccta ctgaggaata
    14881 gacgcgcgtg gtttccagcg attcggccag cgtcatatcg ggcaggatgg tggccatccg
    14941 ctgagccagc atcgtctttc cggcaccggg cggcccaatc atcattacat tatggccccc
    15001 cgccgctgcg atcgtcagcg cccgcttgac gctttcctga cctttgacat ccgaaaagtc
    15061 aacctcatag gccgaggcct gatgaaacaa ggattcaata tcaatgactg tcggctccag
    15121 cggcagcaaa tcggacaaaa aaccgacagc ctgtgccaag gaaccgaccg catagacttc
    15181 tatatcctgc accacagccg cttcggcagc attctcgatc ggcacaagaa tccctttata
    15241 gccgccggca gcagctgtca tcgccatgct gagcatcccg tttacaggac gaatgcggcc
    15301 gtccaaagcc agctccccta caaccacaaa atcgtgcaga cgctcactgg acaatccccc
    15361 tgtgcaggcc agtacaccca gtgctatggg caaatcaaat gcagggcctt ctttgcggac
    15421 atcagccgga gccagattga ccagatacgg caggtcagga tatttgaagc ccgaattgac
    15481 aatcgcactg cggacccgct caatgctttc ctttaccgcc gcatcaggca gacctaccac
    15541 cgtcggctta gacatggccg ccggggatac atccacctcc acctcacaga gaatcccatc
    15601 aatgccttcc agcgtcaccg agaacaattt tgccagcata cccatgcctt tccgctgccc
    15661 ttttccaacc gcagactgcc cggattttac aaaggcggca ttgcactggc aagcattttt
    15721 ctcccagccg cacaggcagg ccgcagacag catttgaccg ctgccgggat tttgtttaaa
    15781 ataagccgtc ggtaattggc ggcttgcaat agaatccgtt tcatggaggc ataggcgttt
    15841 agatggatat gattgaatat ctgcggaaaa gacgaagcgt gcgggcattt gacaatcggc
    15901 cgatagagcc ggacaagatt gcccttctta aagaagccgt tttacgaagt ccctcctccc
    15961 gaaactttga cccgtgggag tttatctttg tcgatgacaa gcagctcctc gagcagctga
    16021 gccgaagcaa gccccacggg gcggagtttt tgaaagatgc cgctgtgggc attgtggtct
    16081 gtgcggatcc agccgtcagc gacgtctgga ttgaagattg cgccattgcc tctattctgg
    16141 cgcagttgac agcccaatcc ctcggattgg gcagctgctg gattcaaata cggcagcgct
    16201 tttacaacag ccggatgtcc agcgaaacat acatccgcaa tgttctgggc attcccgaag
    16261 ccctttgtgt tctctgtatt attgctgtcg gctatccggc ggaatacccc cagcccattc
    16321 cgcgccggca gctcaaagac gctaaaattc acgtcaatcg catgcagtcg gccagatagc
    16381 ggaccacagc atcttcatca ttgctgccga taaccaaatc cgcgcatcgc ttgagctggt
    16441 cttcggcgtt ggcaaccgca acagccctgc cggctgtctg gaacattttt atgtcgttga
    16501 tgtggtcgcc gaagacagta atgctgtcga gggtatgtcc ggtcatccgg gccagcatgc
    16561 tgacggcctt cgacttgcag gccttctggt cgtgaatggt cagccaccaa tgaccgggcg
    16621 tatagaaatt ctcaaacaga aaattttcca gcagtgccgg atattgcgca tccagatatt
    16681 ccgccagggc ctttacctga tcgggcggcc ccatcacggc aaacgcaatg acctgttctg
    16741 ccagagcatg cctcaggtcg ctgatgcgcc ggattcgctt gtctttttgg acgtgaagca
    16801 tatcgtggta ccactgcatc tgctcgtttt tcaaattttg ccaataaagg caatcttcgg
    16861 tgccgttatg agtaacaata aacggctcga gctgatggtc aagaatatgg tgataaatcg
    16921 actctgcaaa atcccgggca atactgttga tcaccaagtg cctgccggtt ttgtaatcgg
    16981 tcaaaaaagc cccattgacg gctatcaccg gcagcgtcag cggcaaatcc cccaacaccg
    17041 gcacaatttc cccccacgcc cgggcgctgg ccacggtaaa ctgcaggccc gcctgcagca
    17101 attcggtcag cgtctttcgg ctgtaatccg aaagcgtacc gtctgaacgc aacagtgtgc
    17161 cgtccaaatc tgaaacaaaa aaggaatcca ttttttagac agagtttata cctgcattgc
    17221 aatcgccgat ttcagaaact aacatctatg tacaatcaac cgcagcaaaa acgcataccc
    17281 ttatttttgg tatgcagccg gaaccagaca caaaaaacgg gccggatgcg tgccggagcg
    17341 gttgatgaac tgatgttttt cattgggcat aaccaagagg acttggccca tctgcacagg
    17401 gtgagggccg gattcatcga cggcagctat atcgccgtca agaacaacaa cttcatgctc
    17461 gaacgggtgc gaatgaaacg gcgtatggcc gccggggcct aattcaaaaa gccgcatcgc
    17521 aaaggtcggt acctgatcgg ccggtccaaa cagcacccgg actttgacat tctcagcccc
    17581 atccatcgtg acatcctgca gcctaacatc ggtcattttt ttgataatca tataagactc
    17641 ctgccagtgg gaaatttgac tatcagaata caaaatacaa cccaaatatc aagttcaaat
    17701 gcccaaaaag gccgcatgat tcggtgttga ctgttcaggg ccgaatggct gcgtgcggac
    17761 tgcagaggat tatactttcg gcagcactat cgtaactgtt gttcccttgt cgggtttgct
    17821 gtccagcgaa aggcggccgc cgttgagcag aagcagccgc tgagcatgag ccagccccat
    17881 cccgcggcgg cgtccggcgg gacgatagga aaagaacggc tggactgctt ttgccagtgt
    17941 gtctgcatcc attccgcagc cggcatcacg aatcgccacc actgcgctgc gctcatcaga
    18001 gccggcaagg cagtcaatcc agatggggcc gctgccacct ttgtaggact gcagcgcatt
    18061 cattagaata taggacagac tctgaatgac ctgatgcgca tccacgtaaa cagggggcgc
    18121 ctgttccgct ccgctaatgg taatctccag tgcactcagg ccggcttcca tacgcgtttt
    18181 atcgatagct ttccgcagca gctcatcgat tggaacagtt cgcttctgag gagcagccgg
    18241 ctgggcaaaa gccatcaaat ccgagataat ctgcgatatc tcttctgtgc gctgctgaat
    18301 ctggcgaagc atctgctttt tgtcgggatc ttcttcggcc gtcagcagca gctgcgcccg
    18361 gcccgagata accgccagcg gagtattcag ttcatgggcc gctccggcag ccatttgtgc
    18421 cagaccttcc agcgactgcc gacgggtcag ttcggatcgc gcctgacgca gtgtccccat
    18481 catctgaaca aaccgctccg ccatctcgcc attttttcgg acggcctgtg ccatcgctat
    18541 cgcagcagca gcgatgcggc agcacagcag aaccatgctg tctcttgcgg cttcccgtaa
    18601 cggcgaaaaa tcctcataca ccagaaccgc agggatctgg tcgcctatgg acagagaaac
    18661 cattttcatc cgggcagggt caaagtcaca ttcgagctgc tcaagaatcc atttggctgc
    18721 gtctgcagcg ggctgtatgg cagactgatg ctgaatgctg tggggtattg gaggaatgcc
    18781 gtcgggcatc tgaagcagct ttatatctgt ccggccctgc cgatctgtca ccgccgcttc
    18841 tacaaacggc tccgtcgcat ccggcacaac ataaacgcag gtcaaaccgc tttgacaatg
    18901 cgtctgccaa gccgcagcga atgcctcggc gatatcggcc ggcaaggcat tttggtcaat
    18961 agcatccaga aaatcgccaa tgagctttgc ctgagcagtt aacaggcgat gctccatcgt
    19021 tgattcggcc aatttgcggt tgctttcggc caaatgaact gccgttttgc gaatcagcga
    19081 aacatactga cggtcggaac ccgctgcgcc gcccagcccg agggagcggc ttcttcggct
    19141 gaccgtttcg gccagcgaat cagcaatccg ggaaatctgc tcggaattca gtgaaagaag
    19201 ctgcgccagc tctgcaatat cctgcggcgt atcgtaactg ccgctgtgcc cgatgccggc
    19261 ggtgcgcact aagcggtcgg ccagtgcggc aacacgaaca ataggtccat aaggaaggtc
    19321 agcagccagc gtctgagcgt cgcaatgatg cagccagatc gccgctgtga tcgcatcagg
    19381 aagaaaccac ttttctgcca gacgcttgcc gatcagcgca tggtcgagcc ccaaatgttg
    19441 ctgctcgatt tcaatcagac agcagctgcc tgaacgcgcc tgctctacca ttctggcaaa
    19501 acttttgggc atcacctcat caagggcata tttgccgatg tcgtgaagaa gccctgcaag
    19561 ataggccgca gtccgctgtt cctccggaag aacgcattgg gctaattcat cggcacagca
    19621 gccggctgcc agcgcatgca gcgccatttc gcgccggggc agcggacgct gggcatcagg
    19681 actttcgccc ccctcaagca catcaaaaac tttgaccgaa atcagcgctt cgcgcagcag
    19741 tgccgccggc agtttagcgg ccgcttcagc caccgaagga ccgccgctga aacgtacacc
    19801 ttcctgatga gccagcgaca gcaccatagc cgcaagagcc ggatcagcct gaatgtgttc
    19861 agccagagct tccggcccag ccgcactgtc attcagacgg ccgagcaaat cagcggcaac
    19921 cgacgggaat gtggacaaag aatggagctg acgaacaatg cgttcaacct gacgagcgac
    19981 aacttgcgga ttgctgggca tgcagatatc ctttgctgta tatcatccca accaattatg
    20041 aaaccgaaga cagattggac tggtcaacaa cggataaaat cttatccacc agttcggcaa
    20101 tattgaacgg ctttttgaga aactctgccg cacccgcatt cagcagggcc tgaatttcat
    20161 cctgattgac tacgccgctg acgataataa tcttaatgtt ttcaaaatca ggattgcgtt
    20221 tgatggtctg gcagacaatg tttccattca catccggtag catataatcc agtattatca
    20281 catccggcat gaactgctgg gtctgcattc cagcatcata gccgctggag gcggttcgaa
    20341 tctcaaaacg tccatcccgg ctgagaacat ccaccatcag ttctacaatc tcggtatcat
    20401 catcgactat caaaaccttg actttgccgg ttttttgcgc attgagattg tccagcggga
    20461 tgctgttttc ccgcataaac ttaatcaact ggtcacgggg aatgcggcgg aagcgagaac
    20521 cgggtacccg aaagccgcct aaccgccccg aatcgaagca gcggataata gtctgctggc
    20581 tgatattaca gatttgtgcc gcctcacctg tcgtaaaaag attttttatt ttctccattt
    20641 tatgcctctt gcatttgaaa agaccgataa tccttcccag tcgtctcttt ttgcctaaat
    20701 taactatcgg acaaacgggc tgtcaagcat taaaaaaatg taattttcaa tactgaaagg
    20761 cctgttgagg catttactgt gtaaatgaat agtcttttag taaaaatcag cctttttagg
    20821 tacaatagaa attgtatatc cacaatagaa tataaatgca gagtattgag atttggtgtc
    20881 tttttgtcgg tattaagccg gttcagcatc ggcaggcaat ttgccaaaaa tccctgtttt
    20941 atttttcatg aatcaggatt tcatactgct gctcaagcag caggatatgc cgcacctctt
    21001 cttccgccag atccatcagc atcgtgcgga attgaatatc atgggccaag ccgagaagct
    21061 gggcatacag ctgaaatgcc gccctttctt tttggatagc catagccata atctcgaccg
    21121 gattcatatt gcgggcttca tcatccaatt gcagatgttc atcccatata aactccttat
    21181 cttccagctg ttcatttgcc ctgacgcaga aaccctgttt gttcatttcg agtgcaatca
    21241 tctggacatg gcttgtctcc tgctgagcca gtgattctgc cagccggcga gacgccggat
    21301 tctgcatgcg gccggcaagc tcgcggtaaa actgcacgga tgcttcctct ttggataagg
    21361 caaattgaag cacttcgtga atcgacttga attccattag aatgctccat tcagggccgc
    21421 tgcaaggcag tcggctgctt ctgctgtgca gccgtcagca gccgccgcca tattcgtata
    21481 tgccctcttt catcttcctg atctttccga ataatgtttc gcatcggctc atcccaaggg
    21541 gctgcctcaa gcagacgact atagtggctg acggccttgg attcaaccca gatgcccatg
    21601 ttcaggattg cttcagctcc cagcaatcgc gcaatagtcc ccatcacaca acctgccaga
    21661 tgccaagccc accgaaacaa actgggggat aatccgtatt cataaagtcc cacctgaaaa
    21721 tcctgcacat gggtcatctc gttgcacatc gctgcaatca gttcccggtt caattcagta
    21781 ttctggcggc caatctgaca gcggtaaacg gcaacggcca taatctcaag agtgtgcagt
    21841 gtgcgaaggg ccttgcggat tttgtttcgc tcttcagccg tcagggaagt gccccgaaac
    21901 actatatcct gatgtcgcat ttcgggacgc agcacccttg tctcagaaga agcaccatcg
    21961 gccgcagcat ctatgaaatg attgtctgtc atcatagccg ctattatgtc gggcgcaggg
    22021 ggaagtgtca aaactttttg ccgacaagaa atcagccttc aatttcgctg aaacagaatt
    22081 gagatgtccc tgaccgaaat taggacaatc tatatcgctc atcaatgaaa tttgttgatg
    22141 cagcgtatcc catcatcggg actttggcat aacagaccaa acgcatcttc ttgctaattt
    22201 cccttttgca agcatctccg gcgaaccatc gcccttgttc tgccgtaaaa aaagagaggt
    22261 ttttgcctaa aacaggaaag gacagaccca atgacatggt gggaagctga acaaatagcc
    22321 aattatttgg aaaaactgga agaggctctt gaagattggt ctctgaccat ctcccaaatg
    22381 cgcgacgaac tgtcggaatt gtcccgtcat ataaaaaaaa tccaagccga actgccgcga
    22441 acctggcaag catccgaacg gctgttcctt acccagcttt ctgaacgggc agcggcactg
    22501 caggagcata tcagggaacg gctcaaacgc gaaaaaccca gagacagtag gcataaaatc
    22561 tcttattagt ccctcctctt tgtggtatag tatttccctg tgtaatacaa aacaggaacg
    22621 gagatagcgt atgcacattg acacccttgt catgggagat tttcaaacca actgttattg
    22681 tgtcagacca gatgcatcgg caggcagctg tctggttatt gacccgggac tggagccgga
    22741 gccgctggtg cagtttttaa cgaacaagac tatcaaggtt gaagccatta tccttacgca
    22801 tggtcatgcc gaccatatcg gaggcgttga aacacttcga cagtattggc cggacgtgca
    22861 ggtggcaatt cacaaagacg atgcttctat gctgaccgat cctgcggata atctctcgat
    22921 actggccgga tatatggtac aggcccgacc tgccgaggtg cttttagatt cggaagatgt
    22981 ttattatcag gccgctggtc ttcggtttaa aatactgcat acgccggggc acacgccggg
    23041 aggaatttgt ctgtatgcgt cagaggacgg cgttctgttt tccggcgata cattatttgc
    23101 cggctctgtc ggacgtactg attttccggg gggtaattat gcgctgctga ttcagatgat
    23161 cactcagaaa cttctggttc tgcctgacca aacgcggatt taccccggtc acggcccggc
    23221 aaccactctg cggaatgaaa aaaagttcaa cccattcctg atataaaaaa tctgtgatga
    23281 tgctttcttt tctgtttgtg ctggggcgga tttgtgataa tataacagca tccatttgaa
    23341 atattaaaga ctcgaggagt attgaatatg tgcgattcat gcggctgcgg cgaaaaaacc
    23401 tttgaagcaa aggttgcgga agtcattgat tccattcgcc cgatgcttca aaatgacggc
    23461 ggggatattg aattggttgg cattgatgcg gataaaaccg tccgtgttcg cctgcagggt
    23521 gcctgccggg gctgcccggg ggctcaaatg accttgaaaa tgggcgtgga acgtctcctc
    23581 aagcagcgtg tcagcgaagt caagcaagtc gttgctgtca gctaatcggc atcggcgata
    23641 tacaggcact gaacaatatt tacggcacaa tgtgcctgaa gattgcttct ggattgatgg
    23701 ggtcaagaat cccgcgttct gtaatgatag cggttatgtt tgcagacggc gttatatcaa
    23761 aagccggatt atagacctgt atatcctgcg gagccgtgtt ttggcggact tcctgtccat
    23821 cgcgatgctc aacaggaata tcggcaccgg tccggcaggt caagtcaatg gtgctggttg
    23881 gagcagcaat ataaaatgga atcttatttt ctttagctaa aacagataaa
//
LOCUS       DAOTBO010000033        23673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1771_length_23673_cov_5.344695, whole genome shotgun sequence.
ACCESSION   DAOTBO010000033 DAOTBO010000000
VERSION     DAOTBO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 23673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23673
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1771_length_23673_cov_5.344695"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..3353
                     /locus_tag="PLE88_05735"
     CDS             180..3353
                     /locus_tag="PLE88_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM75998.1"
                     /translation="MRTALVCLLAVAAAVYGIGFFVIVPAAQSSIARFCGAGVSIQSG
                     WFTGVGSVRLNGVVVAGETASGDESAILRADRMDVQFIPLHLLKGRLSIHSITLSDFA
                     VTADYDAGQKNWNFSRLAFGARNAGGQTAIPLIQMRRGALRIRRIADTAETIATIGFN
                     GDIAVPAGLREYSFRLETDGRFGYGDSSIQGLLTLGQGDRQSRLAAAGYIRMGQARVL
                     DNAWNLEGIQFECTFDAQTVSIQRCRFLLGQGQADIRGLIHRTGRRPVDLEVSAHGLM
                     TADRFEPDAIVCSRPILSLLEPAAARFLGRYHACGKADIEAQMHGYLDDLSDSQFSGR
                     IRCVDIAVRDDQFPYTVSGIQGDIELKGRTLYLNNLKGRHGDSILLVNGTVSNFGPQA
                     EIDLRTTSESIRFDEDLYNALKDSAKRLWLAFKPQGAAGLDYRFRRAADGRKDAAVAL
                     HLKNASAVYNRIPYLLENLTGTIRIETGRILFENVRASYDDGRSILISGQAEQLQTED
                     PVFSVYVEGRRIPTDAALREALPEAAQALWNRLEISAAADFDIRIQSSPAAKEQFDYT
                     AQVRLQGQRLLYKEFPLAIRDVQLSAEIADDRILLHRLEGRTDNGPVVLSGTVYPAGR
                     LPQQAGFCLELELRDFDLNEAFWNIAGIEQMPGKFRLLGRVDVSGRLETNLPETECRG
                     NDLIAVLKESRLAWEDTPLGRLQGRLRIQNETVKLTDFQWQAIPLESLPLDAAGRQVR
                     ALYAWARPKGQAGLVVHDGCVRMNSQRKPSGIELNAQVKLEGLSCGAAGQVSLGDGVF
                     SGSAAGDFESQRWEVSGDYVINRLQYRHWLAEHVSGRAAYDPNTMRLKGSNLTAQLYD
                     GAVSGEFQVNLAAGVQPGYLLKLDCQDVPVIKLLEARQPIEPQQQMQGLVSGSLTLQG
                     DFQTPAEPRGRLTAAVVDMKMGRQSLPGKILTAVQLRQPEEFVFSRMDVAAVIRGAEL
                     ILDDVVMVGQPLIFRGKGRLNLQSRQITMELTAWNRPPAQEDTFLDRLLRGMGAALWR
                     VEVRGDMKDPVVQAVFLSVFRPPTGLF"
     gene            3415..4656
                     /locus_tag="PLE88_05740"
     CDS             3415..4656
                     /locus_tag="PLE88_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOM75999.1"
                     /translation="MFDVAIIGAGAAGLMAAVYAAQAGAKTCVFEKNSAAGKKLLLTG
                     GGRCNLTHTGSIDDFIKACHPFGNTLRHAFYALRPADLIDFFARRGLETAAEPDGCIF
                     PRRGCAEDVLRILLGEVNRCGAAVYYGARVAEVSKNTGGFQIKTAQASYACKCVVIAA
                     GGASWPQTGSTGDGYKLAETFGHTVIEPIGILCPVVCSQPWPGRLQGISLERVSIRMQ
                     LGGKRQTVTGAAVFTADGIGGPAAFDVSRAAAETLRRGNSVPLWLNFCPDCTAEMLEA
                     QIIRHCAEHPQKDLAGILSLRLPRRIGELLQEMTAADEPVCAGHLRRQQRRRLVELLS
                     AMPLTAIRCAPLEKATVTRGGICRSQIDFRTMQSRLCEGLFFAGEVIDVDGPCGGYNL
                     QIAFSTGALAGMRAAGCARLK"
     gene            complement(4712..6016)
                     /locus_tag="PLE88_05745"
     CDS             complement(4712..6016)
                     /locus_tag="PLE88_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76000.1"
                     /translation="MYSTRMLIYAILLMSLMVPFAPAAPDASVPQVAGKPANVQYPFI
                     AEVTGNNVYIRSGKGPLYYHCGKLNIGNTVTVVEDADGWSKIVPPEGCYSWIHKDYVK
                     VSAGSNKGEVIGENVRVWAGSDFIEASRSDSMQVKLNKNKDQIDEDDFVELLSDQPEN
                     SAYYKIKPPAGAFLWVSSEFLKYVGPLVHKTPVAVPPRPDANEVLPPAEEKTVPAFSN
                     LTPQEPSAQAAGVQEPAAKEAAAPTPPAPKPVTKESVLLKQCYELSAQIDQQLKKPLS
                     EQNYADISKSLEAIKNDPEAGKAASYSQILLERIARYELAIRVTDIVKQQDEALAAAK
                     EQIEKAHQAQLSRLPKTLEFLFVGTLKPSYVYTDKTGQKRYVLMDGSGKILCYVTAAS
                     PEIAARLEQAVDTQIGIRGNIVSNSKALVTLVSATAVEAMPQ"
     gene            6398..7207
                     /locus_tag="PLE88_05750"
     CDS             6398..7207
                     /locus_tag="PLE88_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76001.1"
                     /translation="MAFDAAIKEYLKEIDDAPLLSWEEEKALAKAVREENDPQAREQL
                     IRSNLRLVVNIAKKFSGHGLTLGDLIEEGNLGLMRAVDTFDPEHGVRFSTYSAWWVKQ
                     SIKRAILSNSQPIHIPTYMVELINQWRQVYAELETRLGRVPDLDEMAKAMSVPVRKAQ
                     AIREIVAVVDSGFQHEPTEQNAGLDEAVEDDTIINPEDDLGTNEQLERALELLDQMDT
                     RQAEVLRLRFGLGGQEPLTLKQIGEKLGLTRERVRQLQRNALAQLNALLTT"
     gene            7291..7818
                     /locus_tag="PLE88_05755"
     CDS             7291..7818
                     /locus_tag="PLE88_05755"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOM76002.1"
                     /translation="MPAQAGELKAFIREIPDFPKPGILFRDITPLLAHPQALAQAIEA
                     LAAPFADKGIDVVAAVEARGFIFGSAVARTLGAGFVPIRKKGKLPFHTESVSYGLEYG
                     RDVVEVHTDAIRPGMKVLMVDDLLATGGTMAAACKLIEKLGGTIAGLTFLIELSGLNG
                     RQQLKPYVLHTVICY"
     gene            7833..8942
                     /gene="aroB"
                     /locus_tag="PLE88_05760"
     CDS             7833..8942
                     /gene="aroB"
                     /locus_tag="PLE88_05760"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOM76003.1"
                     /translation="MSKPIQTLDVRIPAAEAGCYTIAVGQGILADVWADIRRRWPNCS
                     PFVVTDAAVAEAGHLKTLLGTAAAPVYVIEPAGEASKHIQTVAAIVEAMEKERLGRDT
                     VVAALGGGTVGDIAGFAAAIFKRGVPVIQIPTTAVAQADSAIGGKTGVDSSVSKNAFG
                     VFWHPAAVYIDVKTLLTLDDRQYRAGLVESVKHAAIADAVYFEYLENNIDAILKKDLS
                     MLEYMAMTNCRIKASVVQEDPTEKNKRRILNYGHTIGHAVEAASGFGLLHGECVAIGM
                     KAAGRIEKAMGLVRDSRLERIETLLRKLQMPAKIPANLKKERILDLLKMDKKAVGQWP
                     RFVLLESLGQVWCKDGQWAADVPTGIVKETVSQLY"
     gene            8960..10702
                     /locus_tag="PLE88_05765"
     CDS             8960..10702
                     /locus_tag="PLE88_05765"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOM76004.1"
                     /translation="MRYSKTLIPTVKEVPADAEIPSHQLMIRAGLMRKLASGTYIYLP
                     AGQRMLAKVMQIVREEMNRTGAQEIIMPVVQPLELWQQTGRDIDYGQTLGTFVDRHGR
                     GNVLSPTAEEGFTYLAAGEIKSYKQLPMNLYQINTKYRDEFRPRFGVLRSREFIMKDA
                     YSFHTSEECLHKTYMDMYHAYCRVFERCGLEYVIVLAESGEMGGTGSHQFTVPCASGE
                     DIIVYTADNRQAWNIEKAPVDPLPKQPPVPVGPVEDFYTPKAGRIDDACAYMKVKPHQ
                     LIKTLIYKSPEGVILALVRGDHEVNTEKLTRLMDGAHIEMADEAAIVELTGAAVGFAG
                     PVGLCGKAAKVFIDYGVAAMAVGATGANKTDYHIMNVVPGRDFPLEGAHIRVADIRNA
                     VEGDTFEGKKLLFKRGIEVGQVFKLGDKYTRKLDVKYVDADGKEKYPLMGCYGIGINR
                     IIASAIEIGHDKDGMILPITIAPWEVIVTPAGQEEEVTAAAERIYQQLLEAGVEVLLD
                     DRDIRAGVKFKDADLLGIPIRITIGKKTLAEGRVECKRRCDSDCTTLPLDTAAAEVAQ
                     IVSRMKDKLNAVKN"
     gene            10869..11414
                     /locus_tag="PLE88_05770"
     CDS             10869..11414
                     /locus_tag="PLE88_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOM76005.1"
                     /translation="MWDIKTPKPAKLIVGILACSRQAAEASQLPLTEAFGPADLVSQI
                     WPFDMTEYYADQAGPNIVRQFLAFETLLDPGRLAWVKHQTNQMEQELARRLNTPYPRP
                     VNLDPGLVEPSKLVLASTKNFAHRIYIGEGIYAEVTMTYVRGRWETFPFTFPDFKSGR
                     YNEFLNLVRQAVVRQQRELEK"
     gene            11544..12653
                     /locus_tag="PLE88_05775"
     CDS             11544..12653
                     /locus_tag="PLE88_05775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOM76006.1"
                     /translation="MAINHLTIAALVLPAAACLCSGLLTACVRRWAMRTDFVARPRED
                     RYHRSVVALGGGIAIFWTIALFVLIPAAVVKFVIAPGHLSLVDSSVARHIPGFIDKSG
                     GLLVVIAAALILHLLGLWDDKKRLGPFVKLTFQFAAALLAAVWADIRVEMFIENKLIT
                     TVLSVFWIVLMINAFNFLDNMDGTSAGIALIAACVLMAAAFRSGQVFVGAMGLVFIGA
                     LAGFLVFNFPPAVIFMGDCGSMVVGFFVALLTLRTTYYDPAVGTPLYSVFMPAVVMAV
                     PLYDFISVTFLRLKQGKSPFVGDTQHFSHRLKRRGLTDRQVALTLYLATLCTGLGAVG
                     LQQADLLGAVLIAAQTVMVLIFIAILESAGSREAS"
     gene            12650..14959
                     /locus_tag="PLE88_05780"
     CDS             12650..14959
                     /locus_tag="PLE88_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOM76007.1"
                     /translation="MKASVHPPAQRPPVLQRLDHILLCVMMGLIVLRAAVIENPHLDQ
                     PQTRLMLSAEVVSLLISAVLLVCFAVWLLAAVLFNQFRWRKAGFGPAAALFFLAGLLS
                     AFFASDKRAAVTDAVTLLCPMLTALLLVQLLDTRQSIRLVLLLLAAVGAAAAVQCADQ
                     LMTANQMLIADYQADPVQHLQKLGIEPNSLEHWMYEHRLYSKDIRGFLMTSNSSASFF
                     LLAFFAAFGLFTQAVAKDSAQEQTAAAVCYGLAAAVCGAGVLMTQSKGGIGALLLGLL
                     LWLVLFGFGQRIWQHRRLAGAALLLAVLLTAAGIIAWGIRYGRLPGGNSMLVRWQYWQ
                     STAQMIRDHLPTGVGGGNFSESYMYYKNPAASETIQNPHSWPLSLLSQYGPLGLLAFV
                     WGAFGPMLKSFRSRFTLPDNSGQSQSAQSGLLWAGLLACCGCMLLFVRPALVDTEFLY
                     QNVEVRSAAYLVLYLVPAGVFVLAYVLLWAASTGDRSPAGRSDRLNTALLCGLAAVLI
                     HNLIDFAVFEPGIWSIFWLLTAVLMADFHLAGGQTPSVIHLDAPVRLGAAAGLALVCA
                     VYAVGVLLPPVRANQLLKQALRDDRRRIELLDRAIACDPLSPKTAYTAAAMFTQTYRS
                     QRVKDVRFLEKALDFAEIAGRRNPASFKPFRLRGEIYSLLAQQADGTRKTLYLEKAAE
                     AFRMALERYPGSDRMHFALAAAAEQLHQPQTALQHYQRAVEIEDAYRIQFRTMYPDRQ
                     TVISRLGHQAYQEAKEKIQTLGGAGETGR"
     gene            15051..16169
                     /locus_tag="PLE88_05785"
     CDS             15051..16169
                     /locus_tag="PLE88_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOM76008.1"
                     /translation="MNNRLPKVLGAVCLLVCASAAAAESRFVPDSVWPDNNGVHINAH
                     GGGVLYYHDAYYWFGEHKTAGRRGNTAQVGVGCYSSKDLYNWKDEGIALAVSSDPNSE
                     IVRGCIIERPKVVYNSKTNTFVMWFHLELRGQGYRAARTGLAVSDSVTGPYRYLRSFR
                     PNARQWPVNAQQQDKMAGQENYLQRDFEIGQMARDMTVFVDDDGKAYHIHAAEENYTL
                     HISELTDDYLDFTGRYARVLPWGHNEAPAVVKHQGRYYLLTSGCTGWAPNAARSAAAE
                     HIFGPWTELGNPCRGVNPHNNLGPEKTFGGQSTFILPVQGKKEAYIAMFDVWVPSNPI
                     DGRYIWLPVRFEDGRLVIEWLDEWDLSVFDKSAARGIR"
     gene            complement(16330..22494)
                     /locus_tag="PLE88_05790"
     CDS             complement(16330..22494)
                     /locus_tag="PLE88_05790"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOM76009.1"
                     /translation="MAQSIRWFLDREKQMNSTAKRPFYIGLAALLFCFTTAWGDLFDP
                     PLKNPSFEDNPLAPGAVTYTFNEWHDISGWVEYTTGPEGNGLPWTPYGNIWAGFIGNP
                     TGTNTGLIYQQVGTWSENMGCEVIFLVGKRAGKAPFPIRIALWAGGNATSAADGVSLT
                     AIGATMIDSVTISPDFGGQDVATLESHVFLSTGTGFSATDALWLEFKSMGTINSQMYI
                     DNVQIRAAVLCNTPVPANGAVRIQPAPVLSWQEPPLEEDASYTLYFGPNPDIASNPKW
                     TDVHPPFHPGTLEYMTTYYWRIDTVAGDTVFMGDDWTFTVGGKTSDPNPANGAVNLPM
                     TDKNLMWSGDSWATSYKVYAGTQFPLLYLGEVLTPIYPHYPTPYELTTYYWRVDEYVN
                     GQLAVEGDVWQFSTRQRMDACPEGDLSGDCVVSLADILLMAQQWLNPFGCDGFAAACA
                     DLVGQDGVNLSDMAVISSNWQRRGESAIVINEIHYNPDLKTERVEFIELYNTGPFDVD
                     MSGWRFCDGVDYTFPQGTIFPAESYLVIAEDASQAYRPVTISQKYGTPSNLVMGPFSG
                     SLNNEGENIKLCDAQGNTVDQVDYGLGFPWPTVGDAVPDVAPGGTGHSIQLIHPKLDN
                     NLGGSWRSAYPTPGDMNTAVFAENSPPQIRQVAHFPQQPKSGQVVTITAKVTDPDGVA
                     SVVLRYQLVDPGSYIPITLPNLNTATPTVPNPDYENPANWTAAAMYDNGQNGDAVAGD
                     SVYTVQIPASVQVHRRLIRYRITAADTAGLSVRVPYADDPQPNFAYFVYDGVPAWSGA
                     INPSGSAPLNEVQHFNPVVMRSLPVYHLIARETDVLTCQYNSSWDDTQYHFGGTLVYD
                     GKVYDNIHYRVRGQASTFMWGKNKWKFDFNRGHYFQARDDYGRPYAKKWDKMNVGMGG
                     CPWWQYPHPGPWDQGAGGLFLNETLAYRLYNMAGVPASHTQYFQFRIIDAADENGSTQ
                     YSGDFWGLYLAIENPDGAFLDEHGLPDGNLYRMDSGYEKKNQGATQIKDNSDIAAFVS
                     SSTGYNKTNPYQPLSWWQANTDLQTYYSSKATGIAINDSDRRPEANCIYYSNPASGKW
                     MMLPWDLDLTFEWGTHYTDWEHWRYVLSYAEANIEYQNRCRELLDLLFNPEQAGQVVD
                     EIAAVISDSDLTKSFIEAERAMWDYHPRISKKGQWYANNEFLTSAYGGTQDWAGMLTY
                     YKTVLSPTGYNNGYTYGVKALTAEAQDASIPYKPTVSYIGSNGYPSNDLRFQTSPFSD
                     PQGADTFAAMKWRIAEVRPFVKQPLPTITGGTAEPTPITLIAQQSVWKYFKGTAEPSL
                     PVENWRQNGFNDNEWLTGQTSIGMQDNDDNTELSDMYKSYYTVYLRNTFDISDVSRIQ
                     NLTLHVYVDDGCIIWINGTEVGRLYCPDGFIAYNQAIAYNHEATSYETVILTAPYTYL
                     VNGTNTIAVQAIQSSTSSSDFSIDVSVTAQLAAQTSPEPFYEDSYAYRVTKGKYEIDA
                     VWESPELTSFSSTITIPGSVVRPGRTYRVRCRMKDNTGRWSHWSSPVEFAAGQPLAGG
                     VLDSLRITELMYNPAADASGAYDSQEYEFIELKNIGSKTISLNSVTISDGITFDFAAA
                     PSALQTLQAGQYVLVVKNKAAFEFRYGTGVSDRIAGQYSGKLSNSGETLVVSDFWNGT
                     IVRFTYNDGFGWPTAANGGGHSLIVKDSAVEEEPLGTLDYGGNWRASTYRHGSPGQDD
                     PAPVVDVRLNEVIAHTDYSEPAHPEYDSNDYIELFNTSNYSVELNGYWYLSDDINQLK
                     KWPLPASTLAAGGHIGYDEVSGFHNPITQGFGLDKAGEQVFLSYLPGTSQDRIVDCIR
                     FRGQENGVSLGRYPDGGDDWFVMPPSRNAANAVPIQDVVISEIMYHPLEGSSNDEYIE
                     LYNPTSASILLANADGSWRLSNAVSYVFPAGISLAAGQRLIVVPFDPTDIIRLEAFKT
                     AYGADSLTPAVNVFGPYIGALSNGGERLALERPQAPDAIGQQVSWVQVDEVYYGTFSP
                     WPVQADGLGKSLVRISSESSASGNNPANWCDGTPSPGR"
     gene            complement(22633..23319)
                     /locus_tag="PLE88_05795"
     CDS             complement(22633..23319)
                     /locus_tag="PLE88_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76010.1"
                     /translation="MKSKCYAMVLALLVISSGLVSAATVIGVNFCDAWDKPHMEGKTA
                     DGFDNWTDSWPLWNWDNPSSGSVVVRGSNDLVTCTWSSNNTWAGGLEDTSEQQLYRVY
                     LDDTGNGALVTISGLGNWLASIGAAGYTVRIYHSTDNGSGFVGVDLTDGTSILETVQE
                     TNHWTTDGGVRAFVDSGVLSASTLVLDPLPRDGANRATIAAFKITAIPEPATLLLLAA
                     GGLVIRRKHS"
BASE COUNT         5237 a         5995 c         6878 g         5563 t
ORIGIN      
        1 aggggcttac ttccggcggg atattgccga taaggcgctg cgacctcttg ggcaataaaa
       61 gataagcagc cggacctaaa ggaccaagcc ggacgcttgc cgtgtccgat aacagctcca
      121 tgtcaagtca tacaagaaca aaaaggcttc gcaaaatccg acggcgaatt tttcggtctg
      181 tgcgcactgc gctggtgtgt ctgctggctg ttgcggcggc ggtgtatgga ataggatttt
      241 ttgtcattgt gcctgctgca caaagcagca tagcgcggtt ttgcggcgcc ggtgtatcca
      301 ttcagtccgg ctggtttacc ggagtcggtt cggtccgcct gaatggggtt gtcgtggcgg
      361 gcgaaactgc atcgggggat gaatcggcga ttttgagggc ggatcgcatg gatgtgcagt
      421 ttattccgct gcatttgctc aaagggcgtc tgtcaattca ttcgataaca ctgtcggatt
      481 ttgcagttac ggccgattat gatgccggcc agaaaaattg gaatttcagc cggctggcgt
      541 tcggggctcg gaacgcaggc ggacagacag ccattccgct gatacaaatg cggcggggtg
      601 cgcttcgcat ccgccggatt gccgatactg cagaaacgat tgcgactatt ggattcaacg
      661 gcgatattgc ggttccggcg ggtttgcggg aatacagttt ccggcttgaa acagacggcc
      721 ggtttggcta tggcgacagc agcattcaag gtctgctgac gctggggcag ggcgatcggc
      781 aaagccggct ggcggcggca ggatatatcc ggatggggca ggcacgggtg ctggataatg
      841 cctggaacct tgaggggatt cagttcgagt gcacatttga tgcccagacc gtttcgattc
      901 agcggtgccg ttttttgctg gggcaggggc aggccgatat tcggggcttg attcatcgca
      961 caggccgccg tccggttgat ttggaggttt ccgcgcacgg tttgatgacg gctgaccggt
     1021 ttgagccgga cgccatagtg tgcagccggc cgattctgtc gctgctggag ccggcggcag
     1081 cccggttctt agggcggtat catgcgtgcg gcaaggccga tatcgaagcc cagatgcacg
     1141 ggtatctgga tgatctgtcg gattcccaat tcagcggccg gatacgctgc gtggatatag
     1201 ccgtccggga tgatcagttt ccctatacgg tcagcggcat tcagggagat attgagctca
     1261 aaggccgtac gctttatctg aacaatctca aaggccggca tggggacagc attctgctgg
     1321 tcaatggaac agtcagcaat tttggacctc aggccgagat agatcttcga acaaccagcg
     1381 agagtatccg ttttgatgag gatttgtaca atgccctcaa ggattcggct aagcggctgt
     1441 ggctggcatt caagccgcag ggggcggcgg ggctggatta tcgttttcgc cgggcagcgg
     1501 atggacgaaa ggatgccgct gttgcactgc atctgaagaa tgcttcggct gtgtataatc
     1561 ggattccgta tctgctggaa aatctgacag gaaccatccg catagaaacc ggccggattc
     1621 tgttcgaaaa tgtcagagcc agttatgatg acggccgcag cattctgatc agcgggcagg
     1681 ccgaacaact gcagacagag gatccggtat tcagcgttta tgttgaaggc aggcggattc
     1741 cgacggatgc ggcgcttcga gaagccctgc cggaggcagc acaggcactg tggaatcgtc
     1801 tggaaatctc tgcggctgct gattttgata tacgcatcca atcttctccg gcggccaaag
     1861 agcagttcga ttacaccgcg caggttcggc tgcagggcca gcggcttctg tataaggaat
     1921 ttcctcttgc gatacgggac gtgcagctgt cagcagaaat tgctgatgac cgtattttgc
     1981 tgcatcggct tgaaggccga accgacaacg gccctgtggt gctcagcgga acggtttatc
     2041 cggcaggccg tctgcctcag caggcgggtt tctgtctgga gctggagctg agggactttg
     2101 atttgaatga ggccttttgg aatatcgccg gcatcgagca gatgccggga aaattccggc
     2161 tgctgggccg agttgatgtg tcgggacggc tggagacgaa tcttccggaa acagaatgcc
     2221 gcggcaatga tctgattgcg gtgctcaagg agagccgatt ggcgtgggag gatactccgc
     2281 tggggcgttt gcaaggccgg ctgcggatac aaaatgaaac tgtaaagctg accgattttc
     2341 agtggcaggc tattcccctt gaatcgctgc cgctggatgc ggcaggccgg caggttcgcg
     2401 ccctgtatgc gtgggcacgt cccaaaggtc aggcagggct tgtagtgcat gatggatgcg
     2461 tgcggatgaa ttcccaaaga aagccctcgg ggattgaatt gaatgcccag gtaaaactgg
     2521 aagggctttc gtgcggtgcg gctggacagg tttcactggg ggatggtgtt ttcagcggat
     2581 ctgcagcggg agattttgaa tcgcagcggt gggaagtttc cggcgattat gtgatcaacc
     2641 ggcttcaata ccgccactgg ctggcagaac atgtgtccgg ccgggcggct tacgatccca
     2701 ataccatgcg gctgaaggga tcaaacctca cagcgcagct gtatgacggt gcagtcagcg
     2761 gtgagtttca ggtcaatctt gccgccggcg tccagccggg atatctgctg aagctggact
     2821 gtcaggatgt ccctgtcata aaactgctgg aagcccggca gcccatagag ccccagcagc
     2881 agatgcaggg attggtttcc ggttcgctga cattgcaggg ggattttcag acccctgcag
     2941 aaccgcgcgg ccggctgact gcagcggtgg tggatatgaa aatgggccgt cagtcgctgc
     3001 cgggcaagat tctgacggct gtgcagctac gccagccgga agagtttgtt ttcagccgaa
     3061 tggatgttgc cgccgttatc cgcggagcag aactgattct ggatgatgtg gtgatggtgg
     3121 ggcagccgct gatttttcgc ggcaaagggc gtttgaatct ccaaagccgg cagataacga
     3181 tggaactgac cgcatggaat cggccgcctg ctcaagagga tacttttttg gatcggcttc
     3241 tgcggggaat gggtgcggca ttgtggcggg tggaggtccg cggcgatatg aaggatcctg
     3301 ttgttcaggc agtttttctg tctgttttca ggccgccgac aggacttttt taaggccggt
     3361 gaggcagctg gttttgggag acgcatttac cgaacttttg aatgcacaac agcaatgttt
     3421 gatgtagcta ttatcggggc aggagcagcg ggtctgatgg cggctgtcta tgcggcacag
     3481 gccggcgcca agacctgtgt tttcgagaaa aactcggctg ctggcaaaaa actgctgctg
     3541 acgggcggcg gacggtgcaa tttgacgcac accggcagca tagatgattt catcaaagcc
     3601 tgtcatccct tcggcaatac cctgcggcat gctttttatg cgctccgtcc ggccgatttg
     3661 atcgatttct tcgcccggcg cgggctggag acagcggctg aacccgacgg ctgcattttt
     3721 ccgcgcaggg gctgtgcgga ggatgtcctc aggattcttt taggcgaagt caaccgctgc
     3781 ggcgcagccg tttattacgg tgcacgggtc gcggaggttt ccaaaaacac agggggcttt
     3841 cagattaaaa cagcacaggc gtcctatgcc tgcaagtgtg tggtgattgc cgccggcggg
     3901 gcttcatggc cgcagacggg ctcgaccggc gacgggtata agcttgccga gaccttcggt
     3961 catacagtta tagagccgat agggatatta tgtccggttg tatgcagtca gccatggccg
     4021 ggccggctgc agggcatcag tttggaacgg gtatcaattc ggatgcagct gggcggaaaa
     4081 aggcagactg tcacgggtgc ggctgtgttt acagccgacg gcatcggagg accggcggcc
     4141 tttgatgtca gccgggctgc cgccgaaact ctccggcggg gcaattccgt cccgctttgg
     4201 ctgaatttct gccccgattg cactgctgaa atgctggaag cacaaatcat ccggcattgc
     4261 gcagaacatc cccaaaaaga ccttgcgggg attttgtcgc tgcggttgcc gcggcggatc
     4321 ggcgagcttt tgcaggagat gacggcggca gatgaaccgg tttgtgccgg ccatctacgc
     4381 cgccagcagc ggcggcggtt ggtcgaactg cttagcgcta tgccgctgac ggcaattcgc
     4441 tgtgcgccgt tggagaaggc aacagtgact cgcggcggaa tctgccgcag ccagattgat
     4501 ttcaggacga tgcagtcgcg gctgtgcgag gggttgtttt ttgcaggcga agtgattgat
     4561 gtcgatggtc cctgcggggg gtataatctg cagattgctt tttcaacggg tgcattggcg
     4621 ggaatgagag ccgccggctg tgcccggctg aaataaaaaa gccccgtcgc ctgagtcgcc
     4681 ggggctgttg ggctgtctgt tttgttcgtt tttactgagg cattgcttcc acagctgtgg
     4741 cagaaaccag cgtaaccagt gccttggaat tgctgacgat attgccgcga atgccgattt
     4801 gcgtatcgac ggcctgttca agccgtgcgg caatttcggg tgaagcggcg gtaacatagc
     4861 agagaatttt gccggagccg tccatcagaa cataccgctt ctggccggtc ttgtcggtat
     4921 aaacatacga aggtttcagc gtgccgacaa agaggaattc cagcgtcttg ggcagcctgc
     4981 tgagctgggc ctgatgggct ttttcaatct gttctttggc agctgccagc gcttcatcct
     5041 gctgcttgac gatatcggtt acgcggatgg ccagttcgta gcgggcgatg cgctccagca
     5101 gaatttgcga gtaggatgcg gctttgccgg cttcggggtc gtttttgatg gcttccagcg
     5161 atttggagat gtcagcgtag ttctgttcgc tgaggggttt tttgagctgc tggtcaatct
     5221 gggccgacaa ttcgtagcac tgcttgagaa gcaccgattc ctttgttact ggcttgggtg
     5281 caggcggtgt cggcgcagca gcttccttag cggccggctc ctgaacaccg gcggcttggg
     5341 cagaaggttc ttggggagtc aaattgctga atgccggaac ggttttttct tccgcgggag
     5401 gcaggacttc gttggcatca ggacgagggg gcacagcgac cggtgttttg tgcaccagag
     5461 ggccgacata cttcaggaat tcactgctga cccacaggaa agctcccgcc ggcggcttga
     5521 ttttgtaata ggcgctgttt tcgggctgat cggaaagcag ttcgacaaag tcgtcttcat
     5581 caatctggtc tttattttta ttgagtttga cctgcatgct gtccgaacgg gaggcctcta
     5641 taaagtccga accggcccac acacggacgt tttcgccgat tacctcgcct ttgttcgagc
     5701 cggccgaaac cttgacatag tccttgtgga tccacgaata acacccttcc ggcgggacaa
     5761 ttttagacca gccgtcggca tcttcaacga cggtaacggt gtttccgata ttcagtttgc
     5821 cgcaatgata gtaaagcggc cctttgccgg agcgtatata gacattattg cctgtaacct
     5881 ctgcgatgaa cggatattga acattggccg gcttgcctgc cacctgcgga acagatgcat
     5941 ccggggctgc cggtgcaaat ggaaccatca gggacatcag cagaattgca taaatcaaca
     6001 ttcttgtgga atacattgca atgtctccca atcgaattgt tattttctat atccggactt
     6061 tcctttacga ttttctctaa attcaaaggt tatcactgac tatcttgtct gtcaactgtt
     6121 tttcaagata agcttgggat ggccatggaa catcgtattt ctgaaaaatg gtgtatgcgg
     6181 cgaaaggtaa atattggaga cgttaagata atatggaaga ttctatacag aattactgaa
     6241 agaaaagggc ggccgacggt taactgcatg cacgattcag tcgatataaa ccgcagggtt
     6301 tgctggacaa aacagcagac cctgtcggcc ctataaaaaa gggccttgtg cagacaattt
     6361 ccaaatttcg attgagacgc acggtaggag taaatctatg gcttttgatg cagcaataaa
     6421 agaatatctc aaagaaattg atgatgcccc tttgctcagc tgggaagaag aaaaggcctt
     6481 ggctaaggcg gttcgggaag aaaacgatcc gcaagctcgg gaacaattga ttcgaagtaa
     6541 tctccggctg gttgtaaata tcgcaaaaaa attttccggt catggtctga ctttggggga
     6601 tttgattgaa gagggcaatc tgggtttgat gcgggctgtg gacacatttg atccggaaca
     6661 cggcgtccga ttcagcacct attcggcctg gtgggtcaaa cagtccataa agcgggctat
     6721 tttaagcaat tcacagccga ttcatattcc gacctatatg gtcgagttaa tcaatcaatg
     6781 gcggcaggtc tatgctgagc tggaaacccg cttgggtcgg gtgccggatt tggatgaaat
     6841 ggcaaaagcc atgagtgtgc cggttcgcaa ggcgcaggcc attcgtgaaa ttgtagcggt
     6901 ggtggattct ggatttcagc atgaaccgac agaacagaat gccggccttg acgaagccgt
     6961 agaagatgat acaatcataa acccggaaga tgaccttggc accaatgagc agttggaacg
     7021 ggctctggaa ttgctggatc agatggatac ccgccaagcc gaggtgcttc gattgcggtt
     7081 tggtctgggc ggtcaggaac cgctgaccct caagcaaatc ggcgaaaaac tgggactaac
     7141 gcgggagcgc gttcgtcaat tgcagagaaa tgcccttgcc cagctcaatg cgttgctgac
     7201 aacgtaatca gagaccgatg cagacagccg ataaggcggg gttcgtagga acgcccgcct
     7261 ttttttgttg cagaaagtga ggaaacagcg atgccggctc aagcaggcga attgaaagcg
     7321 tttattcggg agattccgga ttttcccaaa ccggggattt tgtttcggga tattacgccg
     7381 ctgctggctc acccgcaggc gttggcacag gccattgagg cgcttgcggc gccgtttgct
     7441 gacaagggaa tagatgttgt tgcggcggtg gaggcacggg gttttatttt tggctcggcg
     7501 gtggctcgga cgctgggagc cggatttgtg cctatccgca agaaaggcaa gctgcctttt
     7561 cataccgaat cggtcagcta cgggctggaa tacggcaggg atgttgtgga agtccatacc
     7621 gatgctatac ggccgggaat gaaggtgctg atggtcgatg acctgctggc taccggcggg
     7681 accatggccg cggcctgcaa gctgattgaa aagctcggcg gaaccatagc gggactgaca
     7741 tttttaattg agctgtcggg actgaatgga cgacagcagc tgaaaccgta tgttcttcat
     7801 acggtaattt gctattgata gtgaatggga aaatgagcaa gccgatacaa acactggatg
     7861 tccgaattcc ggcggctgaa gcgggatgct atacgattgc cgtcgggcag gggatattgg
     7921 cggatgtgtg ggcggatatt cgacgccggt ggcccaactg ttcaccgttt gttgttacgg
     7981 atgcggcagt ggccgaggcg gggcatttga aaacgctgct ggggactgcc gcggcgccgg
     8041 tgtatgtgat tgagccggcc ggagaggcat ccaagcatat ccagacggtt gcggcgattg
     8101 ttgaggcgat ggaaaaggag cggcttggcc gggatacggt tgttgcagcg ctgggcgggg
     8161 ggacggtggg cgacattgcc ggctttgcgg cagcgatttt caagcggggt gtgccggtta
     8221 tccagattcc caccactgct gtggctcaag ccgactcagc cattggcggc aaaaccggtg
     8281 tggacagctc tgtcagcaaa aacgcctttg gtgtcttttg gcatccagcg gcggtttata
     8341 ttgatgtcaa aacgctgctg acgctggatg accgccagta ccgtgcaggg ctggtcgagt
     8401 ctgttaagca cgccgctatc gccgatgctg tgtattttga atatcttgaa aacaatatcg
     8461 atgcgattct caaaaaagac ctgtcgatgc ttgaatatat ggctatgacc aattgccgga
     8521 tcaaggcgtc ggttgtgcag gaagacccga cggagaagaa caaacgccgg attttgaatt
     8581 acgggcatac catcggccac gccgtcgaag cggccagcgg ttttggtctt ctgcacggcg
     8641 aatgcgtggc catcggaatg aaagccgccg gccgcattga aaaggcaatg ggactggtcc
     8701 gcgatagccg gctggaacgc atcgaaacgc ttttgaggaa actccaaatg ccggcgaaga
     8761 taccggccaa tctgaaaaaa gagaggattc tcgacctgct gaaaatggac aaaaaggccg
     8821 tcggacaatg gccgcggttt gtgctgcttg aatcgctggg gcaggtgtgg tgcaaagacg
     8881 gccagtgggc ggcagatgtt ccaaccggga ttgttaaaga aactgtcagt cagctttatt
     8941 aaaacaggaa agacaacgta tgcgttatag taagactctg ataccgactg tcaaagaagt
     9001 gcccgcagat gcggaaatac caagtcatca gcttatgatt cgtgccggcc tgatgcgcaa
     9061 gctggccagc ggcacctata tttatctgcc ggccggccag cggatgctgg ccaaggtgat
     9121 gcagattgtc cgggaggaaa tgaaccggac cggcgcgcag gaaatcatta tgccggttgt
     9181 tcagccgctg gaactgtggc agcagaccgg ccgcgatatc gactatggcc agaccttggg
     9241 caccttcgtt gaccgccacg ggcggggcaa tgtgctgtcg ccgacagccg aagagggctt
     9301 cacctatttg gcagccggcg aaatcaaatc atacaagcag ctgccgatga atctgtatca
     9361 aatcaacacc aagtaccggg atgaattccg gccgcggttc ggcgtcctgc ggtcccgcga
     9421 atttattatg aaggatgcat acagcttcca taccagcgag gaatgcctgc acaagaccta
     9481 tatggatatg tatcacgcct actgccgggt gtttgaacgg tgcgggctgg agtatgtaat
     9541 tgtgctggca gagtcggggg agatgggcgg taccggctcg catcagttta cagtgccctg
     9601 tgccagcggc gaagatatca tcgtctatac cgccgacaac aggcaggcat ggaatattga
     9661 aaaggcccct gttgacccgc tgcccaagca gccgcctgtt ccggtcggtc cggtcgagga
     9721 tttttatacc cccaaggccg gcaggattga cgatgcgtgt gcctatatga aggtcaagcc
     9781 gcatcagctg atcaaaaccc tgatttacaa gtctcccgag ggcgtcattc tggcgctggt
     9841 acggggtgac catgaggtca atacggaaaa actgacccgt ctgatggacg gggctcatat
     9901 tgaaatggcc gatgaagcag cgattgtgga actaacgggt gcggcggtag gatttgccgg
     9961 gccggtgggg ctttgcggca aagccgccaa ggtctttatc gactacggcg tagcggcgat
    10021 ggccgtcggg gcgaccgggg ctaataaaac cgattaccat attatgaatg ttgtccccgg
    10081 ccgcgatttt ccgctcgaag gagcgcatat acgggtggct gacatccgca acgctgtcga
    10141 aggcgatacc ttcgaaggca aaaaacttct gttcaagcgg ggcattgagg ttgggcaggt
    10201 cttcaaactg ggcgataaat acacccgtaa actggatgtg aaatatgtgg atgccgacgg
    10261 caaggagaaa tacccgctga tgggctgcta cggcatcggc atcaaccgca ttattgcctc
    10321 agccattgaa atcggccatg acaaagacgg gatgattctg cctatcacca tagccccgtg
    10381 ggaggtgatt gttaccccgg ccggacagga agaagaagta accgccgccg ctgagaggat
    10441 ttatcagcag cttctggagg caggagtaga agtgctgctg gatgacaggg atattcgggc
    10501 aggcgtcaag ttcaaggatg cggatctttt gggcattccg attcgcatta ctatcggcaa
    10561 gaaaaccttg gcggaaggcc gggtagaatg taaacgccgc tgcgattcgg attgcacaac
    10621 cctgccgctg gataccgccg ccgctgaggt ggctcaaatt gtcagccgga tgaaagacaa
    10681 actcaatgct gtcaaaaatt aaatgataag acggccccct gatggttttg ccgaaaagag
    10741 attatgttta ttcagttgtg tttgtaattc tgaaaaatgg aatgtgtctg aagcgattta
    10801 agcccgctgc tttcggcagg ctttcagaca cacttcatcc gtttccagat tccggcgatt
    10861 agccatttat gtgggacatt aaaacaccaa aaccagccaa gctgattgtc gggattttag
    10921 cctgcagccg gcaggccgca gaggcctcgc agctgccgct gacagaagct tttggtccgg
    10981 cggatttggt cagccagatt tggccgtttg atatgaccga atactatgca gatcaggccg
    11041 gcccgaatat cgtgcgtcag tttctggcat ttgaaacgct gcttgacccg ggccggctgg
    11101 catgggtcaa gcaccagacc aatcaaatgg aacaggaatt ggcaaggcgg ctgaatacgc
    11161 cgtatccgcg gccggtcaat ctggaccccg gactggtcga acccagcaag ctggtgctgg
    11221 cctcgaccaa gaactttgcc caccgcatct atatcggcga gggtatctat gcagaagtta
    11281 caatgaccta tgtccgcggc cggtgggaaa catttccttt tacatttccc gatttcaaga
    11341 gcggccggta taatgagttc ttaaatctgg ttcgccaagc ggttgtccgc cagcagcggg
    11401 aattggaaaa ataaatttgg tttctgccga aaaaaagcga aaatagaaaa acggttgtcg
    11461 aactgcaaaa tgaaatcgga taacctaaca gagaacggtt gaatcctgtt gaatatcagt
    11521 agaaaaaatc tggtgccgag tgaatggcaa ttaatcattt aacgatagca gcactcgttt
    11581 tgccggcggc ggcatgcctg tgcagcggac tgctgacggc ctgcgtgcgg cgctgggcaa
    11641 tgcggacgga ttttgttgcc cgtccgagag aagaccgcta tcaccgctcg gtcgttgctc
    11701 tgggaggggg gatagcgatt ttctggacca ttgcgctgtt tgttctgata ccggcagccg
    11761 ttgtgaagtt tgtcatcgcc cccggccatt tgtcgctggt cgattcttcg gtggcccggc
    11821 atattccggg atttatagac aaatccggcg gcttgctggt cgtcatagca gcggccctga
    11881 ttcttcatct gctgggactg tgggatgata aaaaacggct gggaccgttc gtcaagctga
    11941 cctttcagtt tgcagcggca ctccttgcgg ctgtgtgggc tgatatccgc gtcgagatgt
    12001 ttatcgaaaa caaactcatc acgacggtgc tgtcggtttt ctggattgtg ctgatgatca
    12061 atgcctttaa ttttctggat aatatggacg gcaccagcgc cggcatcgcg ctgatagccg
    12121 cttgcgtgct gatggcggcg gccttccgca gcggacaggt ttttgtggga gcgatggggc
    12181 tggtctttat cggggcactg gccggctttc ttgtgtttaa tttccccccg gcagtgattt
    12241 tcatgggcga ctgcggctca atggtggtcg gtttttttgt tgcactgctg acactgcgaa
    12301 cgacttatta tgacccggca gtgggaacgc cgctttattc ggtgtttatg cctgctgtgg
    12361 tgatggctgt gccgctttat gattttatca gtgtaacctt tctgcggctc aagcagggca
    12421 aaagtccgtt tgtcggcgat acgcagcatt tttcgcatcg gctgaagcgg cggggactga
    12481 ccgaccgaca ggtagcgctg acgctttatc tggcgactct gtgtaccggt ctgggagcgg
    12541 ttggcctgca gcaggccgac ctgctggggg cggtgctgat tgctgcccag actgtaatgg
    12601 tgcttatttt tatcgccatt cttgaatctg ccggcagcag ggaggcgtca tgaaagcatc
    12661 tgtccatccg ccggcacagc ggccgcctgt cctgcagcgg ctggatcata tcctgctgtg
    12721 tgtgatgatg ggtcttattg tgctgcgtgc ggcagtgatt gaaaatcccc atcttgatca
    12781 gccgcaaacg cgtctgatgc tgtcggcaga agttgtcagt ctgcttatct cagcagttct
    12841 gctggtctgt tttgccgtat ggctgctggc tgctgttctg ttcaatcagt tccgctggcg
    12901 aaaggccggt ttcgggccgg cggctgcctt gttttttttg gccggcctgc tatcggcgtt
    12961 ttttgcttcc gacaagcgtg ccgccgtaac cgatgcggtg actctgctgt gcccgatgct
    13021 gactgctttg ctgctggttc agctgctcga tacgcgtcag tcgattcgtc tggtgctgct
    13081 gctgctggcg gctgtcgggg ctgctgcggc cgtgcagtgc gctgaccagc tgatgactgc
    13141 caatcaaatg ctgattgctg attatcaggc cgatcctgtc cagcacttgc agaaactggg
    13201 cattgagcct aacagtctgg agcactggat gtatgaacac cggctgtatt cgaaggatat
    13261 ccgcggcttt ctgatgacca gcaattcgtc ggcctccttt tttctgctgg cattttttgc
    13321 cgccttcggg ctgtttacgc aggctgttgc caaagacagt gcacaggaac agacagcggc
    13381 agcggtctgc tacggactgg cggcggcggt ctgcggagcc ggcgtattga tgacgcaaag
    13441 caaaggcggc atcggcgccc tgctgctggg gctgctgctg tggctggtgc tgtttggctt
    13501 cgggcagcga atctggcagc atcggcggct ggcgggggcg gcgcttcttt tggctgtgct
    13561 tctgaccgct gcgggaataa tcgcatgggg cattcgctat ggccggctgc ccggcggcaa
    13621 ttccatgctg gttcgctggc agtattggca gagcacagcc caaatgataa gagaccactt
    13681 gccaaccggc gtcggcggcg gaaatttctc cgagtcatat atgtactata aaaatccggc
    13741 cgcttcggaa accattcaga atccccatag ctggccgctg tcgctgctgt ctcaatacgg
    13801 gccgctgggg ctgctggcat tcgtatgggg ggcctttggg ccgatgctga aaagctttcg
    13861 gagccgcttt actctgccgg ataattccgg gcagtctcag tcggcccaga gcggactttt
    13921 gtgggcgggg cttcttgcct gctgcggatg tatgctgctg tttgttcgtc cggcattggt
    13981 cgatacagag tttctttatc agaatgttga agtccgttca gcggcctact tggtgttgta
    14041 ccttgtgccg gcgggggtat ttgtgctggc gtatgttctg ctgtgggcgg catcgacagg
    14101 tgaccgctcg ccggccggcc gcagcgaccg gctgaataca gctttgctat gcggtctggc
    14161 ggctgtgctg attcacaacc tgattgattt tgctgttttt gagccgggaa tatggagcat
    14221 cttctggctg ctgacagctg tcttgatggc cgattttcat cttgcagggg ggcaaacgcc
    14281 gtctgtgatt catcttgatg cacctgtgcg tcttggggca gcggcgggat tggcgctggt
    14341 gtgtgcggtc tatgccgtcg gggtcttgct gccgccggtt cgtgccaatc agctgttgaa
    14401 gcaggccttg cgggatgacc gccggcgaat cgagttgctc gacagagcca tagcctgcga
    14461 tccgctctct cccaagacgg cctataccgc tgctgccatg tttactcaga cctaccgaag
    14521 ccagcgggta aaggatgtgc ggttcctcga aaaagcgctg gattttgcag aaattgccgg
    14581 ccggcggaat ccagccagtt tcaagccctt ccgtctgcgg ggagaaattt acagtcttct
    14641 ggcccagcag gccgatggca cccggaaaac gctttatctg gaaaaggcgg ccgaggcgtt
    14701 tagaatggca ttggagcggt atccgggctc cgaccgcatg cattttgcgc tggcggcggc
    14761 tgccgagcag cttcatcagc cccagacggc tctacagcat tatcaaaggg ctgtagaaat
    14821 tgaagatgcc taccgtattc agtttcgaac catgtatccc gaccgtcaaa ccgtcatcag
    14881 ccggctggga catcaggcct accaagaggc caaagaaaag attcagacgc ttggcggcgc
    14941 cggagaaaca ggccgataaa gagatttaca atgcagcccc gagcacggca ataggacgct
    15001 tgctgccggc gtctatacag caaaatgtct gctgatcagg agaggatgcc atgaacaata
    15061 gactgccgaa agttttaggg gctgtctgcc tgctggtttg tgcttcagcg gcagccgccg
    15121 aaagccgttt tgtgccggat tccgtttggc ctgacaataa cggcgtgcat atcaatgccc
    15181 atggcggcgg ggttttgtac tatcacgatg cctactactg gttcggcgag cataaaacag
    15241 ccggccggcg aggcaacacc gctcaagtcg gcgtaggctg ctacagctcc aaagacctgt
    15301 acaactggaa ggatgaaggc attgcgctgg cagtgtccag tgatcccaac agcgaaatcg
    15361 tccgcggctg cattatcgag cgtcccaagg tggtctataa cagcaaaacc aacacctttg
    15421 taatgtggtt tcatctggaa ttgcgcgggc agggctaccg ggcggcccgg acggggctgg
    15481 ctgtcagtga ttcggttacc ggtccgtatc ggtatcttcg ttcgtttcgt cccaatgccc
    15541 gccaatggcc ggttaatgcc cagcaacagg ataaaatggc cggtcaggag aattatctcc
    15601 agcgggattt tgaaatcggg caaatggcgc gggatatgac cgtttttgtt gatgatgacg
    15661 gcaaggcgta ccacatccac gccgccgaag aaaattacac cctgcatatc tccgaattga
    15721 ccgatgacta tctcgacttt accggccgct atgcccgtgt tctgccatgg gggcataacg
    15781 aagcgccggc tgtggtcaaa catcagggcc gttattatct gctcacttcc ggctgtaccg
    15841 gctgggcgcc caatgcagcc cggtcggcgg ccgcggagca tatctttggg ccgtggacag
    15901 agctgggcaa tccctgccgg ggagtgaatc cgcacaacaa tctcggtccg gaaaagactt
    15961 tcggcggcca aagtacattt atcctgccgg tacagggcaa aaaagaggcc tatattgcga
    16021 tgtttgatgt ctgggtgccc agcaatccca tcgacggccg ctatatctgg ctgccggtgc
    16081 gctttgagga cggccgattg gttatcgaat ggctcgatga atgggatttg tcagtgtttg
    16141 acaagtccgc tgcacgaggt attcgatagg caaatcctgc ctctgaatgc tgaacaggcg
    16201 gcactcaaca accggattgt aaatattgtt tttattacaa acggcggata gccatataat
    16261 cgccgccttt tacctcgaca atccaataag gaaaaaacca gactcccgcc ggcagattcg
    16321 ttttcctgat tatcttccgg gagagggagt cccgtcacac caattggccg gattgttgcc
    16381 gctggccgag gattccgaag aaatgcgcac cagcgacttg cccagcccat cggcctgaac
    16441 aggccaaggc gaaaaagtgc cgtaatagac ttcatcgact tgaacccagg aaacctgctg
    16501 ccctattgca tccggcgcct gcggtcgttc gagggccagc cgttcgccgc cgttggataa
    16561 tgccccgata tacggcccga agacatttac cgctggcgtc agcgagtctg caccataagc
    16621 cgttttgaag gcttccagac ggataatgtc cgtcggatcg aaaggaacta caatcagtct
    16681 ctggccggct gccagtgata ttccggcagg gaagacatag gacacggcat tgctcagccg
    16741 ccatgaccca tcggcattgg ccagcaggat ggatgcactg gtcggattgt acagttcaat
    16801 gtattcatcg ttggaagagc cttccagcgg atggtacata atctcggaaa tcaccacatc
    16861 ctgaatggga actgcattgg ctgcattgcg cgacggcggc ataacaaacc aatcatcgcc
    16921 gccatcggga tagcggccca gagaaacgcc gttttcctga cctcgaaagc gaatgcaatc
    16981 tacaatgcgg tcctgactgg tgccgggcag ataggacaaa aagacttgtt ctccggcttt
    17041 atccagccca aagccctgtg tgataggatt atggaagccg ctgacttcat catacccgat
    17101 atgcccgccg gcagccagag tggacgcggg taacggccat tttttaagct ggttaatatc
    17161 atcagacagg taccaatatc cattgagttc cacagaataa ttcgatgtgt tgaacagctc
    17221 aatatagtcg ttggaatcat actccggatg agccggttcc gagtagtcgg tatgagcaat
    17281 cacctcatta agccgcacat cgacaacagg tgctggatca tcctgcccgg gagaaccgtg
    17341 gcgatacgtg ctggcacgcc agtttccgcc gtaatcgagt gtccccaaag gttcctcttc
    17401 gacagcagag tccttgacta tcagcgagtg tcctccgccg ttggcagcag tcggccagcc
    17461 gaaaccgtcg ttgtaggtaa accgaacaat ggtaccgttc cagaaatcgc tgactacaag
    17521 ggtttcgccg gaattagaca gtttgccgga atattgaccg gcaatccggt cagaaacacc
    17581 cgtgccgtag cggaattcga aggcggcctt gtttttgacc accagtacat actggcctgc
    17641 ctgaagcgtt tgcagggctg agggagctgc ggcaaaatca aaggtaattc cgtcgctgat
    17701 agtgaccgaa ttcagggata tcgtcttaga gccgatattc ttcagttcga tgaattcgta
    17761 ttcctgactg tcataggccc cgctggcatc ggcagcggga ttatacatca gttcggtaat
    17821 gcgaagggaa tcgaggactc ctccggccag cggctggcct gcagcaaact ccacaggact
    17881 ggaccaatga ctccagcggc cggtgttatc tttcatccgg cagcggacac ggtaggttct
    17941 gccgggcctg acaacactgc cgggaatggt aattgtactg ctgaaggaag tcagttccgg
    18001 gctttcccag acggcatcga tttcatattt gcctttggtc acgcgatagg catacgagtc
    18061 ctcgtagaat ggctctgggc tggtctgcgc ggccagttgg gcggtaacac tgacatcaat
    18121 cgaaaagtca gagctgctgg tgctggactg aattgcctgc acggcaatgg tgttggtgcc
    18181 gttgaccaga tacgtgtagg gggccgtcag aatgacggtc tcataactgg ttgcttcatg
    18241 attgtaggca attgcctgat tgtaggcaat aaaaccatcg ggacaataca gccggccgac
    18301 ttcggtgccg ttaatccaga tgatgcagcc gtcatccaca taaacatgaa gcgtcaggtt
    18361 ttggattcgg ctgacatcgg aaatatcaaa agtatttctc aaataaacgg tgtaatagga
    18421 cttgtacata tcggacaact ccgtattgtc atcgttgtct tgcatgccaa tactggtctg
    18481 gcctgtcagc cattcattgt cattaaatcc gttctgacgc cagttttcga caggcaacga
    18541 cggctctgca gtccccttga agtacttcca tacagactgt tgggcaatca gtgtaatcgg
    18601 ggttggctcg gcggtgccgc cagtgattgt cggcagcggc tgtttaacaa agggacgcac
    18661 ttcggcaatc cgccatttca tcgcagcaaa agtatccgcc ccctgcggat cactgaaagg
    18721 cgaagtctgg aaccgcaaat cgttggacgg atagccgttc gaaccgatat aggaaaccgt
    18781 cggtttgtag ggaatggaag catcctgcgc ttcggcagtc aaggctttga ccccataggt
    18841 gtagccgttg ttgtatcccg ttggggaaag gacggtcttg taataggtca gcatgccggc
    18901 ccaatcctgc gtgcccccgt aggcgctggt cagaaattcg ttgttggcat accactgccc
    18961 ttttttgctg atgcggggat gatagtccca catagcccgt tccgcttcga taaacgattt
    19021 ggtcaggtcc gaatcactga tgaccgcagc gatttcatcg accacctgac cggcctgctc
    19081 aggattgaac agcagatcca gaagttctcg gcagcggttc tgatactcaa tgttggcttc
    19141 tgcataactg aggacatacc gccagtgttc ccagtctgta tagtgtgtgc cccactcaaa
    19201 tgtcaaatcc agatcccagg gcagcatcat ccacttgcct gatgccggat tgctgtaata
    19261 aatgcaattg gcctccggtc tcctgtcgga atcattgatt gcaatccccg tcgctttgga
    19321 gctgtaatag gtttgcaggt cggtattggc ctgccaccag gacagcggct gatagggatt
    19381 ggttttgttg tatcctgtcg aactgctgac aaacgccgca atatccgagt tgtctttgat
    19441 ttgtgtagca ccctgatttt ttttctcata cccgctgtcc atgcgataga gattgccgtc
    19501 gggcaggcca tgctcatcga gaaatgctcc atccggattt tcaatcgcca gatacagccc
    19561 ccagaagtcg ccgctgtact gggtggaacc gttttcatcg gcagcgtcaa taatgcggaa
    19621 ctggaaatac tgtgtgtgac tggcgggaac gccggccata ttgtaaagcc gataagccag
    19681 tgtttcattc agaaacagcc cccccgcgcc ctgatcccat gggccgggat gcggatactg
    19741 ccaccatgga caaccgccca tgcccacatt cattttgtcc cattttttgg catagggtcg
    19801 gccatagtcg tctcgggctt ggaagtaatg gccccggtta aagtcaaatt tccatttatt
    19861 cttgccccac ataaatgtcg aggcctgacc gcggacacgg tagtgaatat tgtcatacac
    19921 tttcccgtca tagaccagag tgccgccaaa atgatattgg gtatcatccc atgaactatt
    19981 gtactgacag gttaatacat cggtttcacg tgcaatcaga tgatagaccg gcagggagcg
    20041 cataacgacc ggattaaagt gctgaacctc attgagagga gcactgccgc tggggttgat
    20101 tgccccgctc caggccggca cgccgtcgta gacaaaatag gcaaaattcg gctgcgggtc
    20161 atcggcatag ggaacacgga cactcaggcc ggctgtatcc gccgccgtaa tccggtaccg
    20221 aatcagacgc cgatgtacct gtaccgaggc aggaatctgc actgtataaa cagaatcgcc
    20281 ggcgacggca tctccgtttt gcccattatc gtacatggcc gctgctgtcc agttggcagg
    20341 attttcatag tccggattcg gcaccgtcgg agttgcagta ttgagattcg gcagtgtaat
    20401 gggaatataa ctgccgggat cgaccagttg ataacgcaga acaacagagg ccactccgtc
    20461 cggatcggtt acttttgccg taatagttac cacttgtccg cttttgggct gctgcggaaa
    20521 gtgagccact tgccgtatct gcggggggct gttttcggca aaaactgcag tattcatatc
    20581 gccgggagtc ggataagcac tgcgccagct tccgcccaga ttgttatcta gttttgggtg
    20641 aatcaattga atcgaatgcc ccgtgccgcc gggcgctaca tctggcacag catctccgac
    20701 tgtcggccaa gggaagccca atccataatc gacctgatcg acggtattgc cctgtgcatc
    20761 gcacagtttg atgttttcgc cttcgttatt gagcgaaccg ctgaacggcc ccatcaccag
    20821 attggacgga gttccgtatt tttgggaaat ggtcaccggt ctgtatgcct gactggcatc
    20881 ttcggcaata accaaatacg attccgcagg gaaaatcgta ccttgcggaa aagtgtaatc
    20941 cacgccgtca cagaagcgcc agccggacat atccacatca aaggggcctg tattatacaa
    21001 ttcgatgaac tcaacccgct cggttttcag gtccggatta taatgaattt cattaataac
    21061 aatggcagat tctccccgcc gctgccagtt ggaggaaatc accgccatat ccgaaagatt
    21121 gacgccgtcc tgaccgacca agtccgcaca ggcagcggca aaaccgtcgc agccgaacgg
    21181 attcagccat tgctgagcca tcagcagaat atccgccaag ctgacaacac agtcgccgct
    21241 gagatcgcct tcggggcagg catccatccg ctggcgggtg gaaaactgcc aaacatcgcc
    21301 ttcgaccgca agctggccgt tgacgtattc atccacacgc cagtaatagg ttgtcagttc
    21361 atacggagta ggataatgcg gatagatcgg cgtcagcacc tcccccagat acagcagggg
    21421 aaactgtgtt ccggcataga ctttgtagga tgttgcccag ctgtcgcctg accacatcag
    21481 attcttgtct gtcatcggca gattcactgc cccgtttgca ggattgggat cagacgtttt
    21541 gccgcctacc gtaaaagtcc aatcatcacc cataaaaaca gtatcgcctg caacggtatc
    21601 aattcgccag taataggtcg tcatgtactc cagtgtgccg ggatgaaacg gcggatgaac
    21661 atccgtccat ttgggattcg aggcaatatc aggattaggg ccaaaatata atgtatagct
    21721 ggcatcttct tcaaggggcg gttcctgcca gcttaacacc ggcgccggct ggatacgcac
    21781 tgcaccattg gccggaacag gggtattgca tagaaccgct gcacggattt ggacattatc
    21841 gatgtacatc tggctgttaa tggtccccat gcttttgaat tcaagccaga gggcatctgt
    21901 tgcggaaaaa ccggttccgg tgcttaaaaa aacatgactt tcaagtgtcg caacatcttg
    21961 gccgccaaaa tcgggactga tagttacact gtcgatcatg gttgctccga tagcagtcag
    22021 ggatactccg tcggcggcgc tggtcgcgtt gccgccggcc cagagggcaa tccgtatcgg
    22081 aaagggtgcc ttgccggccc gttttccaac caagaaaatt acttcacagc ccatattttc
    22141 ggaccacgtc ccaacctgct gataaatcaa acctgtattg gtccctgtag gatttccgat
    22201 gaatcctgcc cagatattgc catagggagt ccatggaagc ccattgccct ccggccccgt
    22261 agtatattcc acccagccgc tgatatcatg ccattcatta aaggtataag ttacggctcc
    22321 cggggcaaga ggattgtctt cgaagcttgg atttttaagc ggcgggtcaa ataagtcccc
    22381 ccaagctgtt gtaaaacaga ataataacgc agcaagtcca atataaaacg gccgttttgc
    22441 ggtgctgttc atttgctttt ctctatccag aaaccagcga attgattgtg ccaaaccgaa
    22501 ccccccttat tcatacatac gtcagattcg tttgggaagt taacctcaaa atcgctcaaa
    22561 ttttctattt accaacaaca gcgagccgca cgatatcgtg cggctcgcca caatccattt
    22621 ttcaatcgag gatcaactat gcttccgacg aatcaccaga ccgcccgcag ccagcagcaa
    22681 cagggtagcc ggttccggga tagctgtgat cttaaatgcg gcaatagtcg cccgattagc
    22741 cccgtcccgc ggcaaaggat caagaaccag cgtgcttgcc gacagaacac cggagtccac
    22801 aaatgcccga acaccgccgt cggttgtcca atggttggtt tcctgcaccg tttcaagaat
    22861 cgaagtgccg tcggtcaggt cgactcccac aaaacccgag ccgttgtcgg tgctgtggta
    22921 gatgcggacg gtgtaacccg ctgcaccgat agaggccagc cagtttccca atcccgaaat
    22981 ggtaaccaac gcaccattgc cggtgtcatc cagatacacc cggtagagct gctgttcact
    23041 tgtgtcttcc agaccaccgg cccaagtgtt gttcgaactc cacgtacaag tcaccaggtc
    23101 attgcttccc cgaaccacga cacttccgct gctgggattg tcccagttcc acagcggcca
    23161 ggaatcggtc cagttatcaa acccgtcagc ggttttgcct tccatgtggg gtttatccca
    23221 ggcgtcgcag aaattcaccc caatcacggt tgcagcactg accagaccgg atgagatgac
    23281 caacagggcc agcaccattg cataacattt gcttttcatc tttccttctc ctaacaaaaa
    23341 tgaatactca aacacttcca cccgcgctac acatgcaggc cggattaccg cactgttgta
    23401 tttcaaaccc ttcactggag cccggcgctg ccgggttcca atttactgat tcatcgattg
    23461 cacagactgg aaagccccgc ctgctgcagg ggctttccag tctcgtttta tttcaaaaca
    23521 gacataccat ttatttcaca tccgcttagg ggatacatgt cggtacgatg ttgcaatcca
    23581 gccatgtatt cgcaaaaggc acaaagtcat ataggttgac gcggcaatcg cggaattcat
    23641 tgccgatgcc gtctgggccg gccacatcgt att
//
LOCUS       DAOTBO010000034        23266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1818_length_23266_cov_5.076903, whole genome shotgun sequence.
ACCESSION   DAOTBO010000034 DAOTBO010000000
VERSION     DAOTBO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 23266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23266
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_23266_cov_5.076903"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..816)
                     /locus_tag="PLE88_05800"
     CDS             complement(37..816)
                     /locus_tag="PLE88_05800"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOM76011.1"
                     /translation="MALMWCVLKVLGRASSNTRYLIACLGLILMVLATAVTFMMLGEN
                     NTSTAAVPESIPAQTPAVSKPSPPVETQMVAISYTELTTPPDKSLMQTLTARLEAALP
                     YCVIGWIFGITVLSIWYLGGWCQLQRLRRIGTKTVANAVSQTTAELARLLGVKQAVRI
                     VESVLVQVPTVIGWLKPIILLPAAALTGLDEIQLKAMIAHELAHIKCCDYLVNIAQTV
                     MEILGFYHPAVWWVSRQIRIERENCCDDIAVGLIQNRKDRP"
     gene            complement(908..1186)
                     /locus_tag="PLE88_05805"
     CDS             complement(908..1186)
                     /locus_tag="PLE88_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOM76012.1"
                     /translation="MYKDQKTGNTTTLNLMQIMSEKGLLIRDDESFKHVYKPAVSEEK
                     TQKQIVSDMLDKVFAGSAEQLVMWALSAKGVWPEELKKIRQILDKMEE"
     gene            1578..3185
                     /locus_tag="PLE88_05810"
     CDS             1578..3185
                     /locus_tag="PLE88_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76013.1"
                     /translation="MHTLLFIKKTLFCLFFGLVIVLPWEQASGYSGSQRRERPVRPGM
                     EQDGIQDEQRLRLAEEAQGAMNRINQNLRTILELAQRNQRPAAQVIEEAESILEQSKQ
                     YASVLNDAQKAEFMLLQAWKGFYQKNPVEAVNWSMRACRTNEASQDAWISQALFCMLS
                     DKRPLKPQIERPRPQTQQRRPQVYSRPRRNEEMLAVETKPQPFSEKGILAFDLMGMRD
                     DLLREQFGRLEFRTASGKKVEYRPGTDTLCMLLWQQGSAADANDVRFRGGNNPANFAR
                     NPLEMEPIFADTTQNTDIESQRSYMKMLAQACKNQPNVKFVQMNADASDTAGKVAQEL
                     AKDSSIEEAGALVFAADPASNAGQFTRVNARKPFLMIVNKEGKIKYAGTAGDFVPAFI
                     LTELTGIPIPLEQKTDSGMGGGLEPMLMPELFLQPIADPNARPADPNALRRSSASPAR
                     PAAGFRQLPLEEEVQAEKELSYARDLFMELAQKRGLTYTRGVELCREIIRKYPNTRYE
                     QEARLLLRQVPENQRARYQLTNQELGL"
     gene            3207..4094
                     /locus_tag="PLE88_05815"
     CDS             3207..4094
                     /locus_tag="PLE88_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOM76014.1"
                     /translation="MTLSSKPGEVPTLYITAECLPEAWEKTVIAVWEQGLTIKTQYDK
                     PGDPPSKDATVMIAVADPFKEPRIHKNFPGGPEELEAYRQEVVDGIHDHWIDPAAGKW
                     TYTYHERLAAYSPVSDLRAPHSKRPFAPVNQIQYIIDALAACTHTRRAQAVTWMPTCD
                     PKTDDPPCLQRIWCRLTEADEGFVLNMNTHWRSRDLYKAWFMNAYALTDLQRLIAGRI
                     AEKIGKSVRVGRYVDISDSLHIYGAYFPNAQIEVEKMKSTPFTMRAWDSSHPAFEMMT
                     QEARCKLAQDPDWYAKAKG"
     gene            4100..5356
                     /locus_tag="PLE88_05820"
     CDS             4100..5356
                     /locus_tag="PLE88_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOM76015.1"
                     /translation="MHSSKLHACAAICFCCLLAAIAFAQEWNYPPAQPGSDTKDISFY
                     KASDTEYAAGPRTGRPRNVIVLIGDGMGINHVALARHRAVGAAGRLHMERLPVFGLVR
                     TYSADNPVTDSAAAATAMACGIKTSNGKIGVAPDGTAYDSILERLGKKGWRTGLVATS
                     AITHATPAGFAAHSSKRSAETDIAADLLNARVDVLFGGGRKYWIGPPAGVRDDGRNLI
                     AEAAAAGYQIIYSGSQLTSLKPACVLGLFAEEGMMTFPPEPSLAQMTQTAVGLLSSPA
                     GEWFAPRPRFFLMVEGSQIDWAAHNNDADNCIRQTLLFDLAVKEAMDFAQRNRDTLVI
                     VTADHETGGLILRTTKEKQLRAYWTSKDHTAADVPIYSFGPGSQRFSGMMNNTDIPKR
                     IAELVGIRDFPSVKLSVEQKTAVPMQ"
     gene            5621..6433
                     /locus_tag="PLE88_05825"
     CDS             5621..6433
                     /locus_tag="PLE88_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOM76016.1"
                     /translation="MILWLETGGILLIAAAGIVLGWRAAGSMRARLFAMAAAFAVVGL
                     ILLSRWSSLWDTFPFLIPIAASRLRFTLLAFAVSLGLTAPLGQLQSYASRFATCMVMS
                     IFIVILVSLPFLGPAIIQHDLSALPTRIDADGVCRQTQPFTCGPAAAVTALSRLGIKA
                     AEGQLALQSRTSPIIGTSPWNLCRTINANYQQQGIRCSFLHLDSLEQIPPDSILLAVV
                     RDAPAADHCVAVLAVREHTITVADPVAGLIHIPRDSFNRLWRNCGILLQRTL"
     gene            complement(6660..7856)
                     /locus_tag="PLE88_05830"
     CDS             complement(6660..7856)
                     /locus_tag="PLE88_05830"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOM76017.1"
                     /translation="MKVLVINAGSSSIKYQLFDMPARQIRAKGMIERIGQPDASLVHS
                     YNGSKYSAQVRAENHDAGMAVILSVLTDSKIGVVEKIGEIEAVGHRVVHGGEEFTGSV
                     IINSHVLDSIERFADLAPLHNPANLTGIKAAMHSLPNAVQVACFDTAFHTTIPPVAYI
                     YGLPYEIYEKYRVRRYGFHGTSHRYVARRCAKLMGLDKSQVNIITCHLGNGCSITAVK
                     AGKSVDTSMGLTPLEGVVMGTRCGDIDPAILFYLADKGYDTKQLNAMCNKNSGLLGIS
                     GISNDMRNLLTQMANGSQRAKLAVDIFCYRITKYIGAYTAVLGRLDAIVFTGGIGENN
                     AALRAQICADQPQIGIEIDSSRNQASEPKERLISAGTSRVKVYVIPTDEEAAIAGDTY
                     ELAVKQ"
     gene            8134..8781
                     /locus_tag="PLE88_05835"
     CDS             8134..8781
                     /locus_tag="PLE88_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76018.1"
                     /translation="MGSRNNRRALKVLRYLRQKHSHQAKQIDILCRDMVGAHREFSVK
                     LATLNFAVSFYESLLGCSDMETLLDTAAGRFRAALNGADAAIFLLSDHGFDVHIANAG
                     LFEPVEKGQFANWFTPKLVDTISRMNRICWLEQMLRMGLQAPPGVLKTISLAAAPLGQ
                     FEKGAGFVLIYRPASMPLQPDELSCVAAVAPGLRKAIAGFMFKNSSTSANSGFTI"
     gene            8946..10532
                     /locus_tag="PLE88_05840"
     CDS             8946..10532
                     /locus_tag="PLE88_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOM76019.1"
                     /translation="MIAEYNTAIGMRAAAEVILPYNPLKTVFFIGWFYLCLYAVQRSD
                     NSPLIRDRLRYVSNAAALFIGPLHLIVLFIADTVRRMQDGQLLLPDLPAYVLDSIREK
                     PARLRVRHAGGQPAIELLDTAGRRFDEVYAKQGRAEGQERRIQQHTEQMILEAIHSRA
                     TDILIDPKDESHYSIRYRIDGFLRVREVLESKTCNAIINSLKAIAGMDIAEKRRPQDG
                     SFMARIPEGDVFFRMATSGVLGGEKLAIRVLDQTRQLMDLAQIGFSPRQIKTLLDIVS
                     QPQGMILICGPTGSGKTTTLYALLQTIDFSQRNVITVEDPIEHVMPNVSQIEVNPKAG
                     VTFANSLRSILRQDPDVICVGEIRDAETAQMALQAAQTGHLVFATLHASSNMAAMVRL
                     MDLGIKPLLIASALSVIISQRLVRCLCDYCKGPAELSQSQLEYCLKNNVDQKRILQPG
                     GCGHCGGTGYYGRTALLDIMFMDDSIRTLLCNNQLTPGDMKKQGDHSFIASLWKEGMR
                     LVLAGRTTLDEVKRIASDAG"
     gene            10538..11149
                     /locus_tag="PLE88_05845"
     CDS             10538..11149
                     /locus_tag="PLE88_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOM76020.1"
                     /translation="MDITTGLVIAAGAAGALIGFKKGLYVMFATLFNLLAAVFIAVLS
                     VRPLWSFSTDNELSPGYAAACLLLLFILVFSFLQTAAWLYFLRHRQPYFPLWLDKPAA
                     AVLGYLAGCLTAAMLLLSVCILPFSAQDKISRLLPRDQMKKTAVPYVVKACNFLAWYS
                     LECFDGDAELAADYLLNLAEPKTEEPVRFYIPQEPAKPNLPQP"
     gene            complement(11205..12086)
                     /gene="folD"
                     /locus_tag="PLE88_05850"
     CDS             complement(11205..12086)
                     /gene="folD"
                     /locus_tag="PLE88_05850"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOM76021.1"
                     /translation="MTANIIDGKQTAAQMREELKQKVALLKSKGIRPGLAVVLVGQDP
                     ASQSYVAAKEKACEEIGIYSDDNRLPAETTEQQLLELVGKLNADPRIHGILVQLPLPK
                     HIDENKILLAIHPDKDVDGFHPVNVGKMVVGQECFLPCTPHGVIQLLLRHNVVLDGAE
                     VVVVGRSNIVGKPVANMLLQKSKTGNATVTVCHTRTKDIAAHCRRADIIIAAAGRPNT
                     ITADMVKDGAVVIDVGVNRIEDAGRKCGYRLVGDVDFEAVSRKASLITPVPGGVGPMT
                     ITMLLYNTVQAAERSAV"
     gene            complement(12157..13923)
                     /locus_tag="PLE88_05855"
     CDS             complement(12157..13923)
                     /locus_tag="PLE88_05855"
                     /inference="COORDINATES: protein motif:HMM:NF010032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOM76022.1"
                     /translation="MTKLDPTKMKDWQIAEAAEENMKPLYQLARDLGLAYGELLPVGE
                     KLAKLDYAKILDRLRYAPTGRYIDVTAITPTPLGEGKTTTTIGLVQGLGKLGKRVCGC
                     IRQPSGGPTFNIKGSAAGGGLSQCIPMVPFSIRLTGDIECVTNAHNLAMVALTARMQH
                     EHNYDDERLAKIGLRRLNIDPDNVQIKWAIDFCAQALRNITIGKGGKMDGLEMESGAQ
                     ISVSSEVMAILAVSKNLRDLRERMSKIVVAYDKNGNEVTTADLEVDGAMTAWMLDAIN
                     PNLVQTIEGQPILVHAGPFANIAIGQSSIIADLVATKLCDYVVTESGFGADIGFEKFW
                     NLKCRMSGLKPHAVVIVATIRALKMHGGGPVVKPGKPLGEEYTKENLELLEKGCDNLI
                     AHIEIVRKSGIRPIVCINKFYTDTDAELRLVRRIAEQHHAEAAVSDHWLKGGEGALEL
                     SEKVAAIAQEEKDFRYLYDLEMPHQQRIEKIAVEIYGAKGVTYTDKALAKIRKIDSEP
                     RMRQLGTCMVKTHLSLSHDPDLKGRPTGWELPIRDVMVYEGAGYVVPIAGDIKLMPGT
                     GSDPAFRRIDVDVKTGKVTGIF"
     gene            14357..16747
                     /locus_tag="PLE88_05860"
     CDS             14357..16747
                     /locus_tag="PLE88_05860"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOM76023.1"
                     /translation="MAYAGPLIVFNDNGGWCWYQDERVIVYNNKLIIGSIADASGSGG
                     AARDGDVEVAAYDLVNQSITRFTLHDALNSDDHAAPAFLVRPDGRILSMYAKHGGDVY
                     ARYRITVSPGDTSAWQPEQLYTASAGVTYSNLFRLSSTGKTYNFYRGENYNPNVLISS
                     DDGSSWTYAGRLIRIGTGGTRPYVKYASNNTDKIWFSYTDGHPRDVADNNIYVAYLQG
                     TDIYNAYGTDIGNLSPSEGIAPSAGTIVYDAPADGSQRGWTSDMHLDAAGWPVVAYTT
                     RISNDDHRYRYARFDGTKWTDYQIAYAGQCLYTEENDYTGLITLDPRNPDIVYISANV
                     HPVTGAPLISSADGRRHWEIFRGITDDGGATWRWEYITKNSAVDNIRPVVPIWDDPRT
                     ILLWMRGTYYTYTNYNTQIVGMIDPEAISPDSPEITRQPESAAAPIGQTAMFTVQAVG
                     REPLNYVWYKVDSSGDTAVASGSAAWILSDIQPSDYGWYYCIVSNSAGSAMSSLVRLM
                     PAELTAWWPLDGSYADATGHGYNAMPAGSPVFAAGHTGQAVHFNGSSYLNCTNGSSLT
                     LQNGGTISAWVKADPALQNTAWATVVAKGRWAWRLCRYGFGSGNSISFHFNSPNYEYQ
                     ANGSIPALDNTWHHLAATYDGQSIKLYVDGQLDASAATPELVNSNADPVYIANRSDAA
                     RYWVGLIDDVRIYSFAMDAAAVGLLYQDKSCYQLNPYDVNRDCRIDLDDWMVFAADWL
                     DSGIDPQTQVCSANPQRDLTGPQGAPDCRVNLCEFAQLASFWLDCLRLPASDCVQP"
     gene            complement(16796..16990)
                     /locus_tag="PLE88_05865"
     CDS             complement(16796..16990)
                     /locus_tag="PLE88_05865"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOM76024.1"
                     /translation="MECRKKENLEFCTCSYPGCSNKGLCCECLRYHLQNKQLPGCCFP
                     PEAEKTYDRSFRAFAKAWGL"
     gene            complement(17005..18486)
                     /locus_tag="PLE88_05870"
     CDS             complement(17005..18486)
                     /locus_tag="PLE88_05870"
                     /inference="COORDINATES: protein motif:HMM:NF027805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOM76025.1"
                     /translation="MNRYWMPPFLAGMMVFCVSCSRPAAEQPAAAAAAGTGAAADKTP
                     AAGTVAPAAKPAPAAPAAVPTVTLITLDPGHFHAALVQKTMYPQINPTVYVYAPQGDE
                     LAGHLKLIESYNSRPDNPTHWKEEIYTGADFAAKAFSEKKGNVLVLAGDNQKKIEYIK
                     GGIEAGLHVFSDKPMCINAEGYALLKEAFQTAGQKKLLLYDIMTERYEITTILQKELA
                     HKPEVFGQLKTGSADDPAIVKESVHHFYKVVSGSPLKRPAWFFDTARQGEGIVDVTTH
                     LVDLILWEAFPEQPIAADQTRVEAARRWPTILTAEQFRQVTQTSGFPPYLSGALTADG
                     ALACYANGQIDFTVRGIWARVKVEWNYQAPEGAGDTHFSVMKGTRANLVIRQGKEQNY
                     RPELYVEPAEGVSADDLAAAMFKATLDLQRTWPGVRALRDGGRWHIAVPDRYRIGHEA
                     HFAQVTEKFLKFLEAGRMPEWELSNMLTKYYITTTALGMAKTQ"
     gene            complement(18518..19372)
                     /locus_tag="PLE88_05875"
     CDS             complement(18518..19372)
                     /locus_tag="PLE88_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOM76026.1"
                     /translation="MKAQSSRRDFLKQTGLLAAAAGLGSPQTYSAQVPVPGLASPLNK
                     LGLASYTTRNFSLDQTIQMTQRLGLKYLSIKDVHLPLTASDSDIQASIEKITKAGITP
                     YACGVVYMKNAEQVDRAFEYAKAAGFKIIVGVPNHDLLERVSQKVAQYDIRVAIHNHG
                     PTDKVYPTPQSAYEKIKDLDKRIGLCIDVGHTLRSGIDPAEDIVRFADRLLDVHLKDV
                     TAASEEGTTVEIGRGAANMPAIVKALLAIGYSGVAGFEYEKDAKDPLPGTAESIGYVR
                     GVLAALQK"
     gene            19629..21068
                     /locus_tag="PLE88_05880"
     CDS             19629..21068
                     /locus_tag="PLE88_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76027.1"
                     /translation="MDCKWSVTRRDFLAASGKITAGIAAVGALSSCRSASPAVSAGRI
                     RKANEKINVGIIGIRSRGWALYDDLKQIQDVHIKALCDVDGNILAQRAGELEKELGYK
                     PDTYKDMRRLYEDPSIDAVVIATPNHWHALATIWACQAGKHVYVEKPACHNIWEGRQM
                     IEAARRYDRLVQVGFQNRSIQNVRQAMQFLHEGGIGRVYMARGLCYKRRDPIGKVKEG
                     IGTGPDYQYWVFNRPGKLYSADYMANVDYNMWTGPAELLPFSYNRFHYNWHWIWNYGG
                     GDTHNQGPHQFDVARWGLNKKEHPVKVSSSGGYFGPPSDQETPNIQTANIEYADGTLL
                     VFETRGLSTSSEEGIRVGNLFYGTKGWMHVDGSSWKTFFGPDNEPGPSSQQTEESADP
                     MNASGAGGGGHIGNFIAAVRTGRREVLTAEVEEGVISSMLPIMANIAYRVGHTLEFDG
                     SSEKFKDSRPANKLVKRQGRGEFTIPSYV"
     gene            21084..21788
                     /locus_tag="PLE88_05885"
     CDS             21084..21788
                     /locus_tag="PLE88_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOM76028.1"
                     /translation="MKRLILLAAAGLLIWTAGCAKENNASEWTVLFDGTHVGAWRLLN
                     GQDFPKTGWKIQDNLLAFEPDRQRSGDIITKDQFSDFELELEFKLTSGANSGIKYFVV
                     ESASSGNAGLGLEYQLLDDDRHPDAKAGIGGNRTLGSLYDLIAPAENKIVYPVGQWNT
                     AKIVSVGSHVEHWLNGRKILEFERGSADFMNRIAQSKYKSIAGFGLAPAGHILLQDHG
                     NAVYFRNIRIRTLKPR"
     gene            21807..23087
                     /locus_tag="PLE88_05890"
     CDS             21807..23087
                     /locus_tag="PLE88_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOM76029.1"
                     /translation="MRQIILSALLAACVAASYAQPDSSRPADARRPMPDPAVIEASDG
                     TFYVFATGQGLPVYHSANLVDWVQVGRVFKTPVPEWAARAVPGTRGIWAPDIVKLGDR
                     YCLYYSVSTFGSQRSVIGMAVNKTLDHTSPDYQWVDEGLVIESFPDRCNFNAIDPAVF
                     QCQDGRTFMVWGSYWTGLKMTQINPQTGKPLDNPPQILDVAARPDNPAHAIEGAYVIY
                     RSGFYYLFVSFDSCCDGAESTYKVMIGRSQQPQGPYEDFKGRPMLQGGGTLLLAGHDN
                     WRGPGHNSVLTTEQGCWLVHHTYDTFHLDSQRIIQVRPLYWAQDGWPVAGEPLGPGNP
                     MKAPKTDIKAAQIIGSWRLSADYGDEKIIDFLPSGRVAHRKEAKWSFDGRQLTVFLPG
                     IAADSVTCFVEPSGSSFIGRDSQGVILRAKRLNP"
BASE COUNT         5119 a         6314 c         6269 g         5564 t
ORIGIN      
        1 cgccgcagcg ggacaggcgg tttgtgctgc agccggtcag gggcggtctt tgcggttttg
       61 tatgaggccg acggcgatgt catcgcagca gttttcgcgc tcgatgcgaa tctggcggct
      121 gacccaccag acggcggggt gataaaagcc gaggatctcc atgaccgtct gggcgatgtt
      181 gacgaggtag tcgcagcatt tgatgtgcgc cagttcatgc gcgatcatgg ccttcaactg
      241 aatttcatcc aggccggtca gcgctgcggc gggcagcaag ataatgggct tgagccagcc
      301 gataacggtc ggaacctgca ccagtaccga ttcgacaatg cggacagcct gtttgacgcc
      361 cagcaagcgg gccagttcgg ctgttgtttg cgatacggca ttcgcgacgg tttttgtacc
      421 gatgcggcgg agccgctgca attgacacca gccgcccaag taccagatgc tcaacaccgt
      481 aatgccgaaa atccagccga taacgcagta tggcagagcc gcttccagcc gtgcggtgag
      541 ggtttgcatc agggatttgt caggaggtgt tgttaattcg gtgtaagaaa tggcgaccat
      601 ttgcgtttca acgggcggcg agggttttga aacagccgga gtttgtgcag gaatgctttc
      661 gggtacagcg gcggtagacg tgttgttctc gcccaacatc ataaatgtta ccgccgtcgc
      721 cagcaccatc aaaataagtc caaggcaggc aatgagataa cgtgtattgg aagatgccct
      781 gccgagcact tttaatacgc accacatcag ggccatgatg gacaggccct gccagagaaa
      841 atggatcaag acccagccga tttaccggat ggtttcttcg gataaaaagt gtccaagtgt
      901 gcccatatta ttcctccatt ttatctaaaa tttgacggat tttctttaat tcttccggcc
      961 agaccccttt ggccgacagt gcccacatga cgagttgttc cgctgaaccg gcaaagacct
     1021 tgtcgagcat atcgctaacg atctgttttt gggtcttttc ttcgctgacg gcgggtttat
     1081 agacatgttt gaaggattcg tcgtcccgaa tcagaagtcc tttttccgac atgatttgca
     1141 tcaaattgag cgtggtggtg ttgccggtct tctggtcttt atagatttcg gcgttgacct
     1201 agcggacggt agaagggcct ttagtccaca ggactttcaa tatcgccagt tccttttcag
     1261 tggggtattg tcttttttcc cgtgccatta tttctcctta aaaacgtctt taatatcaga
     1321 cggtctcaac ccgagtctgt tacaaatagt tacgaattta ttcgtacttt ctttttcatg
     1381 ccatatttcc gtcgaaaggg tgcttataag gaagatggca gctgaagtgt atcaagcaca
     1441 taccaaaaac aagaaaaagc gtatcgtgat tacgtttcag tgaaagtttt tctttgtcaa
     1501 atccggcttg ggtggtaaaa ttaaagcatt attatggggt tcataataag tcaggggatg
     1561 ctttgaagga aaagataatg catacgttat tattcataaa aaagactttg ttttgtctgt
     1621 tttttggcct tgttattgtg ctgccgtggg agcaggcatc tggttacagc ggctcccagc
     1681 ggcgcgaacg ccccgttcgt cccggtatgg agcaggacgg cattcaggat gaacagcggc
     1741 ttcgtctggc cgaagaagcc caaggggcga tgaaccgcat caatcagaac cttcggacta
     1801 ttcttgaact ggcgcagaga aaccagcggc ctgctgcgca agtcatcgaa gaggcagaat
     1861 ctattctgga acaaagcaag cagtatgcat cggtcttgaa tgatgcccag aaagccgaat
     1921 ttatgctttt gcaggcgtgg aagggctttt atcaaaaaaa tcctgtagaa gcggttaact
     1981 ggtcgatgcg ggcttgccgg acaaacgaag ccagtcagga cgcttggatt agtcaggcgc
     2041 tgttctgcat gctcagcgac aaacgccccc tgaagcccca gattgagcgc cctcgtcctc
     2101 aaacccagca gcggcgtccg caggtttatt cgcgcccgcg gcgaaatgag gagatgctgg
     2161 ctgtcgaaac gaagccgcag cccttcagtg agaagggtat actggccttt gacctgatgg
     2221 gaatgcgtga tgatttgctg cgcgagcagt ttggacggct ggagtttcgt acggcctccg
     2281 gcaaaaaagt cgaatacaga cccggcacag atactttatg catgctctta tggcagcagg
     2341 gttcggcggc ggatgccaat gatgttcgct ttcggggcgg caataatcct gcaaattttg
     2401 cccgcaatcc gctcgaaatg gagccgatct tcgcagatac gacacagaat accgatatcg
     2461 aaagccagcg aagttatatg aagatgttgg cacaggcctg caaaaatcag cccaatgtga
     2521 agtttgttca aatgaatgca gatgcctctg atacggccgg aaaagtggca caggaattgg
     2581 ccaaggattc ttccattgaa gaagccggag ctctggtttt tgcagcggat ccggcctcga
     2641 atgccggaca gtttacccgg gtcaatgccc gaaagccttt tctgatgatt gtgaataaag
     2701 aaggtaaaat taaatatgcc ggcacggcgg gagatttcgt cccagccttc attctgacag
     2761 aattgacagg aataccaatt ccgctggagc agaaaaccga ttccggaatg ggcggcggct
     2821 tggaaccaat gctgatgccg gaattgtttt tgcagccgat tgcggatccc aatgcccgtc
     2881 cggcagaccc caatgccctg cgccggtctt cggcctcgcc tgctcggccg gccgcaggat
     2941 tccgccagct gccgcttgag gaagaagtgc aggccgagaa ggaattgtcc tatgcccgtg
     3001 atttatttat ggaattggcg caaaaacgcg gattgaccta tacccgaggg gtcgaactgt
     3061 gccgggagat tatccgcaaa tatcccaata cccgctatga gcaggaagcc cgccttctct
     3121 tgcggcaggt tccggaaaac cagcgggccc gttatcagct taccaatcag gaactcggct
     3181 tataatccat ccatggaggt ctctttatga cgttatcctc aaagcccggg gaggtgccga
     3241 ctctgtatat tacagcggaa tgtctgcctg aagcatggga aaagactgtc atagctgtat
     3301 gggagcaggg tttgaccatc aaaacccaat acgataaacc cggcgacccc cccagcaaag
     3361 acgctacggt gatgattgcc gtggccgatc cctttaagga accgcggata cacaagaatt
     3421 ttcccggcgg accggaggaa ttggaggcct atcggcagga agttgttgac ggcattcacg
     3481 atcactggat tgatccggcc gccggcaagt ggacctatac ctatcacgag cgtttggctg
     3541 cctacagccc tgtcagcgac ctccgcgctc cgcacagcaa aagacccttt gcccccgtca
     3601 atcaaatcca gtacatcatc gatgcgctgg cggcctgcac gcatacccgc cgggcacagg
     3661 ccgtaacgtg gatgcccacg tgtgatccta aaaccgatga tccgccgtgc cttcagcgaa
     3721 tttggtgccg gctgacagag gccgatgagg gttttgttct gaatatgaat acccactggc
     3781 gcagccggga tttgtacaag gcatggttta tgaatgccta tgccctcacc gacctgcagc
     3841 ggctgattgc cggcagaatc gcggaaaaaa tcggcaaatc cgtccgtgtc ggccgctatg
     3901 ttgatatcag cgattccctg cacatctacg gcgcctactt tcccaatgct caaatagagg
     3961 tggaaaagat gaagtcaacg ccctttacga tgcgtgcgtg ggattcatcg catccggcct
     4021 tcgagatgat gacgcaggaa gcccgctgca aacttgccca agaccctgac tggtacgcca
     4081 aagcgaaagg ataaacccaa tgcattccag taaattgcac gcctgtgcgg cgatttgttt
     4141 ttgttgtctt cttgctgcaa tagcatttgc ccaagagtgg aattacccgc cggcgcagcc
     4201 gggcagcgat acaaaggata tttcttttta caaggcatcg gatacagagt atgccgccgg
     4261 ccctcggaca gggcggcccc gcaatgttat cgtgctgata ggcgacggaa tgggaatcaa
     4321 ccatgtcgca ttggctcgtc atcgtgctgt gggagccgca ggacggctgc atatggagcg
     4381 tcttccggtc tttggtctgg tgcgcaccta ttcggccgat aatcctgtta ccgattcggc
     4441 tgccgcagct acggcgatgg cctgcggcat caaaaccagc aacggcaaaa tcggcgtagc
     4501 tccggatggt acggcgtatg attctattct ggagcgtctg ggcaaaaagg gctggcggac
     4561 aggattggtg gcaacctcgg ccattactca cgcaacgccg gccggctttg ctgcccacag
     4621 cagcaagcgc agcgcagaaa ccgatattgc tgcggatctg ctcaatgcac gtgttgatgt
     4681 tcttttcggc ggcggccgca aatactggat cggtccgccc gcaggcgttc gggacgatgg
     4741 gcgcaatctc attgcagagg ccgccgcagc gggctatcaa attatctaca gcggcagcca
     4801 gttgacgtcg ctgaagcctg cttgtgtact ggggcttttt gcggaggaag ggatgatgac
     4861 ctttcccccc gagccgtcgc tggcccaaat gacacagaca gctgtcggcc tgctgtcctc
     4921 gcccgccggg gaatggtttg caccgcgtcc gagatttttc ctgatggtgg aaggcagcca
     4981 gattgactgg gctgctcaca acaacgatgc cgataactgt atccgccaga cgctgctgtt
     5041 tgatttggcc gtcaaggaag cgatggactt tgcccagcgc aatcgggata cccttgttat
     5101 tgtaaccgca gaccatgaaa ccggaggcct gattttaagg accaccaaag aaaaacaact
     5161 cagagcatac tggacatcca aagaccacac ggctgccgat gtgccgattt acagttttgg
     5221 ccccggaagt cagcggtttt ccggcatgat gaataatacg gacatcccca aacgcatcgc
     5281 tgagctggtc ggcattagag atttcccctc tgtaaagctg tctgttgaac agaaaaccgc
     5341 tgtcccgatg cagtaaattg ctgttttacc ggccccagag gtctgaaaaa attcaaccgg
     5401 cggctcttag tgtttccgca gcgtccgata aaacattttt tattttaatt gctgccgttt
     5461 tttcctgctc cataatggga tttgtcttca cgatagcaat tgtcttcttg cccgagaaag
     5521 atatttgtgc tattattgaa ttaagcggga ttgatgaaaa gttcaggcgg attttatagc
     5581 ctgctcagtg cttaaaagcc gccaaaacgg acgggagaaa atgatactct ggctggaaac
     5641 aggtgggatt ttgctgattg ctgctgccgg cattgttctg ggctggcgtg cggcgggttc
     5701 gatgcgtgca cggctttttg caatggccgc cgcatttgcg gttgtcggac tgattctcct
     5761 gtcccgctgg agcagtttat gggatacatt tccatttctg attccgattg cggccagccg
     5821 tctgcgtttt acgcttctgg cctttgccgt ctcgctgggg ctgacggcgc cgctgggcca
     5881 gctccagtcc tatgcatcac gttttgccac ctgtatggtg atgagcattt ttatcgtgat
     5941 tctggtcagt ttgccgtttt tgggaccggc gattattcag catgatttat cggctctgcc
     6001 gacccgcatt gatgccgacg gcgtctgccg tcagacccag ccgtttacct gcggcccggc
     6061 ggcggctgta acagccctca gccggctggg catcaaagcc gccgaaggac agcttgcgct
     6121 gcagtcccgc accagcccga ttatcggcac ctcgccgtgg aatctctgcc gtaccatcaa
     6181 tgccaattat cagcagcagg gcatccgctg ttcctttctg catctggatt ccctcgaaca
     6241 gattccgccc gacagtatcc tgctggccgt cgtgcgggat gcgcccgccg ccgaccactg
     6301 tgttgcggtg ctggccgtgc gggaacatac cattacggtt gccgaccctg ttgccggcct
     6361 gattcacatc ccccgagaca gcttcaaccg cctctggcgc aactgcggca tcctcctcca
     6421 gcgcacgctg taaaatttag agtgtgaatt ctttatcaca aaggcacgaa gaaagttgaa
     6481 gcttttatgt agtattatta tcttttcttc tcgctcaggt ccttctgcca atacagtttt
     6541 taggtttgtc ttcccttatg ccttcataat ggagtctatt atccacctac ttcaaatcag
     6601 ccgcaggaga catttaataa aacagataca acctgcccgg cggcagcatg tcccggtacc
     6661 tattgtttga cggccaattc gtaggtgtcc ccggcaatag cggcttcttc atcggttgga
     6721 atgacataaa ccttcacgcg gctggtcccg gcgctgataa gccgttcttt gggctcgctt
     6781 gcctgattgc ggctgctgtc gatttcgatg cctatttgcg gctggtcggc acagatctgt
     6841 gcccgcaggg cggcattgtt ttcgccgatg ccgccggtaa agacaatcgc atccagccgg
     6901 cccaatacgg ccgtataggc gccgatatac ttggtaatac ggtagcagaa gatatccacc
     6961 gccagcttcg cacgctgact gccgtttgcc atttgcgtca gcagattccg catatcattg
     7021 gaaatgcccg aaatccccag caggccgctg tttttattgc acatggcatt gagctgcttg
     7081 gtatcgtagc ccttgtccgc cagatagaac agaattgccg gatcaatatc cccgcagcgg
     7141 gttcccatga ccacgccttc cagcggcgtc agccccatcg aagtatcgac gctcttgccg
     7201 gctttgactg ctgtaatcga gcagccgttg cccagatgac aggtgataat gttgacctgg
     7261 cttttatcca gccccatcag cttggcgcag cgccgtgcaa cataacgatg gctggtgccg
     7321 tgaaagccgt accgccgcac gcggtatttt tcataaatct cgtaaggcag gccgtagata
     7381 taagccaccg gcggaatagt ggtatggaaa gccgtatcga aacaggcgac ctgtaccgca
     7441 ttcggcaggg agtgcatggc cgccttaatg cctgtcagat tggccggatt gtgcaggggg
     7501 gcaaggtcgg caaaccgttc gatagagtcc agcacatggc tgttgataat aaccgagccg
     7561 gtaaactctt cgcccccgtg gacaacccga tggccgacgg cttcgatttc gccgattttc
     7621 tccacaacac cgattttgga atctgtcaaa acagacaaaa taaccgccat gccggcatca
     7681 tggttttcgg cgcggacctg tgccgaatat ttgctgccgt tgtacgaatg caccagcgac
     7741 gcatcgggct gaccgatacg ctcaatcatt cctttggccc gaatttgacg tgccggcata
     7801 tcaaaaagct gatatttgat ggatgaactg ccggcgttga ttaccagtac cttcattgtt
     7861 acttttctgc ctttctgtat ctattatatc taacaataat tttttaaata aactcgaatt
     7921 atgactgtca gaacaatttc cgtcaacggc caaaatgcca taaaaataat gaagaagaaa
     7981 aattgcagca gaccggcaag ctgcccatca gagtccgata aatccaaaaa aacggagtac
     8041 tattttgttt ctaagtcggt taagccgaat agataaaaag ccgaaaaaaa gcataagaat
     8101 aaacgaattg ttcagctcta aaagggaaag catttgggtt ccagaaataa tagacgtgca
     8161 ctgaaagtcc tcaggtatct gcgtcaaaag cacagccatc aggccaagca aatagatata
     8221 ttgtgtcggg atatggtggg ggctcatcgg gagttttccg tcaaacttgc cactttgaat
     8281 tttgctgtat cgttttacga atcgcttctg ggctgcagcg atatggagac gcttctggat
     8341 actgccgcag gccgattccg ggctgccctc aatggtgccg atgcagcgat atttcttctg
     8401 tcggaccacg gttttgatgt gcatattgcc aatgcaggcc tttttgagcc ggtcgagaag
     8461 gggcagtttg caaattggtt tactccaaaa ttggttgaca ccatcagccg gatgaaccgc
     8521 atttgctggc tggaacaaat gctgcgtatg ggcctgcagg ccccgccggg tgttttgaaa
     8581 acaatttcat tggccgcagc accgctgggg cagttcgaaa aaggggcggg tttcgtgctg
     8641 atttatcgtc ccgcatcaat gccgctacag ccggatgaat tatcctgcgt ggcggcggta
     8701 gcgccgggac tgcgaaaggc aatcgctgga ttcatgttta agaacagcag cacttcagcc
     8761 aacagtggat ttaccattta gcaaagccgc tgattgtcct gcacaatctg acacactgct
     8821 gatttctcct gttttaaatg tgcaaaaact gttgcatccg cagtctccgc agagtatcct
     8881 gttaaaggtg tgctgggtct ggagtgcagc aaatttgtta gtgaacggca cgtagcaggg
     8941 agaccatgat agccgaatac aacactgcga taggcatgcg ggcagccgcg gaagtcatac
     9001 tgccgtacaa tcccttgaag accgtttttt tcatcgggtg gttttatctg tgtctttatg
     9061 cggttcagcg ttccgataac agccccctta ttcgcgaccg cctgcgttat gtatccaatg
     9121 cggcggcatt gtttattggt ccgcttcact tgattgtgtt gttcatagct gatacggtgc
     9181 gccgaatgca ggacggccag ctgctgctgc cggatctacc ggcttatgtc ttggacagca
     9241 ttcgggaaaa acccgctcgc ctccgcgtcc ggcatgccgg cggccagccg gcaatcgaac
     9301 tgctggatac cgccggccgc aggtttgatg aagtgtatgc caagcagggg cgtgccgaag
     9361 gccaggagcg ccgcatccag cagcacaccg agcagatgat tctggaggcc attcattcgc
     9421 gggctaccga tattctgatt gatcccaagg atgaatccca ctattccatc cgttatcgca
     9481 ttgatggatt tctccgtgtt cgggaggtgc tcgaaagcaa aacctgcaac gctattatca
     9541 acagtctcaa agcaattgca ggaatggata ttgcagaaaa gcgccgccct caggacggct
     9601 cttttatggc gcggattccg gaaggcgatg tctttttccg gatggcaacc tccggtgttc
     9661 tcggcgggga aaagctggcc atccgtgtat tggaccagac gcggcagctg atggatcttg
     9721 cccaaatcgg tttttctccc cgacagatta aaacgctgct ggacattgtc agccagccgc
     9781 agggtatgat actcatatgc ggaccaaccg gcagcggcaa aacgaccact ctgtatgcgc
     9841 tgcttcaaac aatcgatttt tcccagcgca atgtcattac cgtcgaagac cccatcgaac
     9901 atgtgatgcc caatgtcagc cagatagagg ttaaccccaa ggcgggagtt acctttgcca
     9961 actcgctgcg gagcattctc cggcaggatc cggatgtgat ctgtgtcggc gaaatccgtg
    10021 acgccgaaac cgcgcaaatg gcgctgcagg ccgcccaaac ggggcatctg gtttttgcca
    10081 cactgcatgc cagcagcaat atggcggcca tggtgcgtct gatggatttg ggcatcaagc
    10141 cccttctgat agcttctgcg ctgtcggtga tcatctcgca gcggctggtg cggtgcctgt
    10201 gcgattactg caaagggcct gcggagctgt ctcagagtca gctggagtat tgtctgaaaa
    10261 ataatgtgga tcagaagcgg attctgcagc ccggcggctg cgggcattgc ggcggaaccg
    10321 gctactatgg gcgaacggcg ctgctggata taatgtttat ggatgattcc atacggacgc
    10381 tgctgtgcaa caatcagctg acgcccggcg atatgaagaa gcagggcgac cacagtttta
    10441 tcgcctcgct gtggaaagaa ggcatgcgct tggtgctggc aggccggacc actctggatg
    10501 aagtcaagcg aatcgcttcg gatgcaggat aacggaaatg gatataacaa caggactggt
    10561 tattgcggcg ggggctgcag gggcactgat cggctttaag aaggggctgt atgttatgtt
    10621 tgccacgctg tttaatctgc tggcggctgt ttttatcgct gtgctttcgg tccgtcccct
    10681 gtggtctttc agcacggata acgaactgtc ccccggctat gcagcggcct gcctgctgct
    10741 gctgtttatt ctggttttta gcttcctgca gacggccgca tggctgtatt tcctgcggca
    10801 tcgtcagccg tattttcccc tctggctgga caagcccgca gcagctgtgc tggggtatct
    10861 ggccggctgc ctgaccgctg ctatgctgct gctgtctgtc tgcattctgc ctttttctgc
    10921 acaggacaag atcagccggc tgctcccgcg cgaccagatg aaaaaaacag ccgttccata
    10981 tgttgtaaag gcctgcaatt ttctggcgtg gtattcgctg gaatgttttg acggcgatgc
    11041 cgaacttgcg gcggactatc tgctgaatct ggctgaaccg aaaaccgaag aaccggtccg
    11101 cttttatatc ccgcaggagc cggccaaacc caatctcccc caaccgtgac tgccctgtgt
    11161 ccggcagcgg agtgcccccg cagccgacgt gagaacagcc tgtttcagac agcgcttctt
    11221 tcagcggcct gaacggtatt atacagcagc atcgtgattg tcattgggcc gacaccgccg
    11281 ggaaccggtg taatcagcga tgccttcctg ctgacggctt caaaatccac atcaccgacc
    11341 agacgatatc cgcattttct gcctgcatcc tcaatgcggt tgacccctac atcaatcacc
    11401 accgcgccgt ctttgaccat atcggctgtg atggtattgg gccggccggc cgcagcgata
    11461 atgatatcgg cacgccggca gtgagcagcg atgtctttgg tgcgggtatg gcagacggtt
    11521 acggtggcgt tgccggtttt gctcttttga agcagcatat tggccaccgg tttgcccacg
    11581 atattgcttc gcccgacaac caccacctct gccccgtcga gcacaacatt gtgacgcagc
    11641 agaagctgga tgaccccgtg cggcgtgcag ggaagaaaac actcttgccc gacaaccatt
    11701 ttgccgacat tgacggggtg aaaaccgtcc acgtccttgt ccggatgaat ggccagcaaa
    11761 atcttgttct cgtcaatatg cttcggcagc ggaagctgaa ccagaatgcc gtgaatgcgg
    11821 ggatcggcat tgagcttgcc gaccagttcc agaagctgct gttcggttgt ttcagccggc
    11881 agacggttgt catcgctgta aatgccgatt tcctcgcagg ccttttcctt tgcggcaaca
    11941 taagactgcg acgccggatc ctgacctacc agcacaacgg ccagcccggg tctgattcct
    12001 ttgcttttca gcagagcgac tttctgcttg agttcttccc gcatctgggc ggctgtctgt
    12061 ttgccgtcaa taatgttcgc tgtcatggat ctttattctc ccggtaatac cttgctgcct
    12121 gcattgtaag gattcggcgg cgtttggcaa gaaatatcag aaaatgcctg taaccttgcc
    12181 ggttttcaca tccacatcga tgcgccgaaa tgccggatca gagccggttc cgggcatcag
    12241 cttgatatcg ccggcaatcg gcacaacata accggccccc tcataaacca tcacatcgcg
    12301 aatcggcagc tcccagccgg tcggacggcc tttgaggtct gggtcgtgcg ataaagacaa
    12361 atgggtttta accatgcagg ttcccaattg ccgcatcctc ggttcgctgt cgattttgcg
    12421 gattttagcc aatgccttat cggtataggt aactcctttg gctccgtaaa tttcgacggc
    12481 aatcttttca atccgctgct gatgcggcat ctccaagtcg tacagatacc taaagtcttt
    12541 ttcttcctgt gcaatcgcag ccactttttc agacagttca agtgcccctt cacccccttt
    12601 gagccaatga tctgaaacag ccgcttcggc gtggtgctgt tcggcgatgc ggcgaaccaa
    12661 tcggagttcg gcatcggtgt cggtgtagaa cttgttgatg cagacgatcg gacggatgcc
    12721 ggatttgcgg acaatctcga tatgggcaat caggttgtcg catccttttt cgagcagttc
    12781 cagattttcc tttgtatatt cctcacccaa cggcttgccg ggcttgacga ccgggcctcc
    12841 gccgtgcatc ttcaaggccc gaatggtggc aacaatgacg accgcatggg gcttcagccc
    12901 ggacatccgg catttaagat tccagaattt ttcaaatcca atgtcagctc cgaaaccgct
    12961 ttccgttacg acataatcgc acagttttgt ggcaacaagg tcggcgataa tgctgctttg
    13021 gccgatggcg atattggcaa aggggccggc atgcacgagg atcggctggc cttcgatagt
    13081 ctgcacgaga ttcggattaa tggcatcgag catccaagct gtcatcgctc catcgacttc
    13141 cagatccgcg gtggttactt cgtttccgtt tttgtcgtag gccacaacaa ttttagacat
    13201 tcgctcccgc agatcccgca gatttttgga aaccgctaaa attgccatca cttcgctgga
    13261 cacggatatc tgggctccgg attccatttc cagtccgtcc attttgcccc ctttgccgat
    13321 ggtaatgttg cgaagggctt gggcgcagaa atcaatcgcc catttaatct ggacattgtc
    13381 cggatcgatg ttgagccgcc tgaggccgat tttagccagc cgttcatcgt cgtagttatg
    13441 ctcgtgctgc attcgggctg tcagcgcaac cattgccaga ttgtgcgcat tggttacaca
    13501 ttcaatatcc cccgtcagcc ggatcgagaa gggaaccatt gggatacact gagacaatcc
    13561 cccgccggcc gcagacccct tgatattaaa tgtaggaccg ccgctgggct gccgtatgca
    13621 cccgcagacg cgcttgccca gcttaccgag cccctgtact aatccgatgg tagtggtcgt
    13681 tttaccttcc cccagcggcg tcggggttat cgccgtcaca tcaatatatc ggccggttgg
    13741 agcatagcgg agacggtcga ggattttggc gtaatccagc ttagccagtt tttcacccac
    13801 cggaagcaat tcgccgtatg ccaatcccaa atctcttgcc agctggtaaa ggggcttcat
    13861 attttcttcg gcagcttcgg caatctgcca gtctttcatt tttgtcgggt ccaattttgt
    13921 cattgtcgca tccaaaagtt tcaatcaggt tttatctgtg catctgcaag ccgttaaaaa
    13981 cattgaaatt atgccatgtt aaagagttat cgactatctc tggatggaac ttttttaata
    14041 ctattttgct ttttattggg tattgtggtc agagaaagta taaaacacaa cagtattttg
    14101 ctgagccagt ttctggtcaa gaatagtcgt gtatctatat acagtataaa agattgacaa
    14161 tctgcggctg tttttgataa aatagataag ttgtcgataa cggtcagtta atactctttt
    14221 ttgtgggggg cagtttttga gaatagcagc agcaacaaag ctgtttcaag cagcagtatc
    14281 tattctttcc gctttgttta gatttctatg ttgccgtccg ataatccgtt tattcttgtt
    14341 gttggtcttg ggtttgatgg cttatgccgg cccgctgatt gtgtttaacg acaacggcgg
    14401 atggtgctgg tatcaggatg agcgcgtcat tgtctataac aacaagctga ttattggctc
    14461 tattgccgat gcctctggaa gcggcggagc ggctcgtgac ggcgatgtcg aagttgccgc
    14521 ctatgatttg gtgaaccagt cgatcacgcg ttttactttg catgatgcgc tgaattccga
    14581 tgaccatgcc gcccctgcat ttttagtgcg gcctgacggc agaatcctgt cgatgtatgc
    14641 caaacacggc ggcgatgtct atgcccgtta ccgcatcacc gtcagccccg gcgacaccag
    14701 cgcttggcag ccggaacagc tctatacggc ctctgccgga gtaacctatt ccaatctctt
    14761 tcgcctcagc agtacgggca agacctacaa cttctaccgc ggcgaaaact acaaccccaa
    14821 tgtccttatt tcttcagacg acggcagcag ctggacctat gccggccggc tgatccgaat
    14881 cggcaccggc ggcacccgcc cctatgtcaa atatgcctcc aacaatacgg ataagatatg
    14941 gttctcttat accgacggcc atccgcgcga tgtggcagac aataatatct atgtcgccta
    15001 tctgcaaggg acagacatct acaacgccta tggcacggat atcggcaatt taagcccttc
    15061 ggaaggcatc gccccttcag ccggaaccat cgtttatgat gcccctgccg atgggagtca
    15121 gcgcgggtgg acatccgata tgcatttgga tgccgccggc tggcctgtgg ttgcctatac
    15181 aacccgcatc agcaatgacg accatcgcta tcggtatgcc cgctttgacg ggacaaaatg
    15241 gaccgattac cagattgcct acgccggcca atgcttatat acggaggaaa atgactatac
    15301 cggcctgatt accctcgacc cgcgcaatcc cgatattgtc tatatttccg ccaatgtgca
    15361 tcctgttaca ggagccccct tgatttcctc agccgatggc cggcgccatt gggagatttt
    15421 ccgcggcata accgatgacg gcggggcaac gtggcgatgg gaatacatca caaaaaattc
    15481 tgctgtggac aatatccgtc ctgttgtgcc catctgggat gacccgcgga caattctttt
    15541 atggatgcgc ggcacgtatt acacctatac caattacaat acccagattg tggggatgat
    15601 agatcccgag gcgatttcac cggactcgcc cgaaattacc cgccagcccg aatcagcggc
    15661 tgcaccgata gggcaaacag ccatgtttac ggtacaggcc gtcggccggg aacccctgaa
    15721 ttatgtgtgg tacaaagttg acagcagcgg cgacacagca gtagcctccg gttcggctgc
    15781 atggattctg tccgatatcc agccgtccga ttacggctgg tattactgca ttgtgtccaa
    15841 ttccgccggt tcggctatgt catcgctggt ccggctgatg cctgcagagt tgacggcgtg
    15901 gtggccgctg gacggcagct acgccgatgc gaccggtcac ggctacaatg ccatgccggc
    15961 aggaagcccc gtctttgccg cagggcatac aggacaagcc gtccatttca acggcagcag
    16021 ttatctcaat tgcaccaacg gttcttcgct gacgctgcag aacggcggca ccatctccgc
    16081 atgggtgaaa gccgatccgg ccctgcagaa tacggcttgg gcgacagtgg tggctaaggg
    16141 ccgctgggct tggcggctgt gccgctacgg ttttggctcc ggcaattcca tctcattcca
    16201 cttcaattcg cccaactatg agtatcaggc caacggcagc atccctgctt tggacaatac
    16261 atggcatcat ctggccgcca cctacgacgg ccagagcatc aagctctatg tggatgggca
    16321 attggatgcg tccgcagcga ctcccgaatt ggttaacagc aatgccgatc ctgtctatat
    16381 agccaatcgc tcggacgccg cacgctactg ggtcggactg atcgacgatg tgcgcatcta
    16441 tagtttcgca atggacgctg cggcagtcgg actgctgtat caggacaaat cctgctacca
    16501 gctcaatccc tatgatgtca acagagactg ccggattgat ctggatgatt ggatggtttt
    16561 cgccgccgac tggctcgaca gcggtatcga cccgcagacg caggtctgca gcgccaatcc
    16621 gcagcgggac cttaccggtc cgcagggcgc ccccgactgc cgggtcaatc tctgcgaatt
    16681 tgcccagctg gcttccttct ggcttgactg cctgcggctt ccggcatccg actgcgtcca
    16741 gccgtaaagc ggttatcggc cggctgctga cgcctgcttt cggaacgctg accggttaca
    16801 gcccccatgc cttggcaaat gccctgaagc tgcggtcgta ggttttctcg gcctccggcg
    16861 ggaaacagca gcccggcagc tgtttgttct gcaggtgata ccgcaggcat tcgcagcaaa
    16921 gccctttgtt gctgcagccg ggatagctgc aggtgcaaaa ctccagattc tccttttttc
    16981 tgcattccat aagcggtctc ggtcctactg tgttttggcc atacccagcg ctgtggtggt
    17041 aatgtagtat ttggtcagca tattggatag ttcccactcg ggcatccggc cggcttccag
    17101 aaacttcagg aatttttcag tcacctgtgc aaaatgcgcc tcatgcccga tgcggtagcg
    17161 gtcgggaacg gcaatatgcc agcgtccgcc gtcccgcagt gcccgcacgc ccggccaagt
    17221 gcgctgcaga tccagcgttg ccttaaacat ggccgccgca agatcgtctg ccgatacgcc
    17281 ttcggccggc tcgacataca gttccggccg gtagttctgc tctttgccct gacgaatcac
    17341 cagattggcg cgggtgcctt tcatcacgga aaagtgcgta tcgccggcgc cttcgggggc
    17401 ctgataattc cactcgactt ttacccgtgc ccagatgccc cgcacggtga aatcaatttg
    17461 accattggca tagcaggcca aggcgccgtc ggcagtcaag gcgccgctta aataaggcgg
    17521 aaagccgctg gtctgcgtca cctgacgaaa ctgttcggct gtcaggattg tcggccagcg
    17581 ccgggcggct tccacgcggg tctggtctgc tgcaatcggc tgttcgggaa atgcttccca
    17641 taaaattaaa tccaccagat gggtggtcac atccacgatg ccctcgccct gacgggcggt
    17701 atcaaaaaac cacgccggac gcttcagcgg actgccggaa accaccttgt agaaatgatg
    17761 cacgctttcc ttgacaatcg ccggatcgtc ggccgaaccg gtcttcaatt gcccgaagac
    17821 ctccggcttg tgcgccaact ctttctgcag gatggtggta atctcgtacc gctcggtcat
    17881 gatgtcatac agcagcagtt ttttctgccc agccgtctgg aaggcctctt tcagcagggc
    17941 gtatccttcg gcgttgatgc acatgggctt gtcggaaaag acatgcagcc ctgcttcgat
    18001 gccgcctttg atgtactcga ttttcttctg attgtcgccg gccagcacca gcacattgcc
    18061 tttcttttcg gaaaatgctt tggctgcaaa atcggctcct gtatagattt cttccttcca
    18121 atgcgtcgga ttgtccggcc ggctgttgta gctttcgatg agtttcagat ggccggccag
    18181 ttcatcgccc tgcggtgcat agacatacac cgtcggattg atttgcggat acattgtttt
    18241 ctgtaccagt gcagcatgaa aatgccccgg atccagcgta atcagcgtca ccgtcggcac
    18301 ggcagccggt gcagcggggg cgggtttggc tgccggggcg actgtgccgg ctgccggcgt
    18361 tttatcggct gctgcgcctg tccctgctgc ggcggccgca gcaggctgtt cggcggccgg
    18421 cctgctgcac gatacacaaa aaaccatcat tcctgcaaga aacggaggca tccaatacct
    18481 gttcatagct ttcctttctc cgaatggttt ggtcaatcta tttttgcaag gccgccaaca
    18541 caccccgcac gtatccgatg ctttcggcag tacccggcag gggatctttg gcgtcttttt
    18601 cgtattcaaa gcctgcaaca ccgctgtagc caatcgccag cagggccttc actatggcag
    18661 gcatattggc tgccccgcgg ccgatttcga cggttgtgcc ttcctcggaa gcggcggtaa
    18721 catccttcag atgcacatcc agcaggcggt ctgcaaagcg gacaatatcc tccgccggat
    18781 caatgccgct gcgcagcgta tggcctacat cgatgcaaag cccaatccgc ttgtccagat
    18841 ccttgatctt ctcgtacgcg ctttggggcg tggggtaaac cttgtcggtt ggcccgtgat
    18901 tgtggatggc cacccggata tcatactgcg caaccttttg gctgacccgc tcgagaaggt
    18961 catggttggg caccccgaca attattttaa atcccgcggc cttggcatat tcaaaggccc
    19021 ggtccacctg ttctgcattt ttcatatata caacgccgca ggcatacgga gtaatgccgg
    19081 ctttcgttat cttttctatc gaggcctgaa tgtcgctgtc cgatgcagtc aggggcaaat
    19141 gcacatcttt aatggacaga tacttcaatc ccagacgctg ggtcatttga atcgtctggt
    19201 ccagcgagaa attgcgggtt gtataggaag ccaatcccag cttgttcaaa ggagatgcca
    19261 gacccgggac cggcacttgt gcgctgtaag tttgggggct gcctagccct gcggcggctg
    19321 ccagcaggcc ggtttgtttt agaaaatccc gacgtgatga ttgtgctttc attgtgctgc
    19381 ctctgtgaat taaggtcgct tttttaaaat aacggttacc ggtgcctatg gcaagcatca
    19441 aacggcaaag atactgcaat gtcaagaaaa attcatcacg gcgtgaggaa tttcctcttt
    19501 ttggcttgac aatgcagcag caataacgca acaataggca cacagattaa aatcaaacag
    19561 attaaagcga aaagtccccc aacggctttt cgcagaagaa tccttaaaat ccgaaaagga
    19621 gaacaacaat ggattgcaaa tggtctgtta cacgccgcga ctttttggct gcttcgggca
    19681 agattaccgc cggcattgct gctgtgggcg cattgagttc ctgccgttcc gcttctcccg
    19741 ctgtctctgc cggccgcatt cgcaaagcca atgagaaaat caatgtcggc attatcggca
    19801 ttcgcagccg cggatgggcc ttgtacgacg acctcaaaca gattcaagat gttcatatca
    19861 aggccctctg tgatgtggac ggcaatatac tggcccagcg ggccggcgag ctggaaaaag
    19921 aactcggcta taagcccgac acctataaag atatgcgccg gctctatgag gatccgtcaa
    19981 tcgatgctgt tgtaattgcc acaccaaatc actggcatgc tctggcgaca atctgggctt
    20041 gtcaggccgg caaacatgtg tatgtcgaaa agccggcctg ccacaacata tgggaaggac
    20101 gccaaatgat tgaagccgca cggcgctatg accgtctggt tcaggtgggt ttccagaacc
    20161 gttccatcca gaatgtccgt caggcgatgc agtttctcca cgaaggcggc attgggcggg
    20221 tctatatggc acgcggcctg tgctacaaac gccgcgaccc catcggcaag gtcaaagagg
    20281 gcattggcac cggtccggat tatcaatatt gggtatttaa tcgccccggc aagctgtatt
    20341 cggccgatta tatggccaat gtcgattaca atatgtggac cggtccggca gagctgctgc
    20401 cgttcagcta caaccgcttc cactacaact ggcactggat ctggaattac ggcggcggcg
    20461 acacccacaa tcagggaccg catcagtttg atgttgcccg ctggggcctg aacaagaaag
    20521 aacatcccgt caaagtcagc tcttccggcg gctatttcgg tccgccgtcc gatcaggaaa
    20581 cccccaacat tcaaacggcc aatatcgaat atgccgacgg cacattgctg gtctttgaaa
    20641 cccgcgggct ttcaaccagc agcgaagaag gcatccgcgt gggaaatctc ttttacggca
    20701 ccaagggctg gatgcacgtg gacggcagct cgtggaagac cttctttggg ccggacaacg
    20761 aacccggccc ctcctcgcag caaaccgaag agtcagccga cccgatgaat gcctccggcg
    20821 ccggcggcgg cgggcatatc ggcaatttca ttgcagccgt ccgtaccggc aggcgtgaag
    20881 tgctgacagc cgaagtcgaa gaaggggtga tctcgtcaat gctgccgatt atggccaata
    20941 ttgcctaccg cgtggggcat acgctcgagt ttgacggctc cagcgaaaaa ttcaaagaca
    21001 gccgcccggc caacaagctg gtcaagcgtc agggccgagg cgaatttacg attcccagtt
    21061 atgtttaagc aaaggatgca aaaatgaaac gactcatact gctggctgcc gccggcctgc
    21121 tgatctggac tgccggatgt gccaaagaaa acaacgcttc cgaatggaca gtgctgttcg
    21181 atggaaccca tgtcggtgct tggcggctgc tgaacggtca agactttcct aaaaccggct
    21241 ggaagattca agacaacctg ctggccttcg aacccgacag gcagcgcagc ggcgatatta
    21301 tcaccaaaga ccagttcagc gattttgaac tggagctgga attcaagctc accagcggcg
    21361 ccaacagcgg catcaagtat tttgtggtgg aatccgccag cagcggcaat gccggactgg
    21421 ggctggagta ccagctgctg gatgacgaca ggcatcccga tgccaaagcc ggcatcggcg
    21481 gcaaccgaac cctcggttcg ctctatgacc tcattgcgcc ggctgaaaac aaaatcgtct
    21541 atccggtcgg ccaatggaat accgccaaga ttgtctcggt cggcagccat gtggaacatt
    21601 ggctcaacgg ccgcaaaatt ctcgagtttg aacgcggctc agcggatttt atgaaccgga
    21661 ttgcccagag caaatacaag agcatcgccg gctttggtct ggcgccggcc ggccatattc
    21721 tcctgcagga tcacggcaac gcggtgtact tccgaaatat ccgcatccgc accctgaagc
    21781 cccgataacc tgttgggagt tggcatatga gacaaatcat tctgtcagcg ctgctggcgg
    21841 cctgcgtggc tgcaagctat gcccagccgg acagttccag accggcagac gcccgccgcc
    21901 cgatgcccga tccggctgtg atagaggcct ccgacgggac attttatgtc tttgcaaccg
    21961 gtcaggggct gccggtgtac cattcggcca atctggtgga ctgggtgcag gtaggccggg
    22021 tctttaagac gcccgttccc gagtgggctg cccgggcagt gcccggcacc cgtggcatct
    22081 gggcgcccga tattgtcaag ctcggtgacc gctattgtct ctattacagc gtctcgacct
    22141 tcggcagcca gcgctcggta attggcatgg ccgtcaacaa aactctcgat catacctccc
    22201 cggattatca gtgggtggat gagggattgg taatcgaatc ctttccagac cggtgcaatt
    22261 ttaatgccat tgatccggct gtctttcagt gtcaggacgg ccgtaccttt atggtatggg
    22321 gttcctattg gaccggcctc aagatgaccc aaatcaatcc ccaaacaggc aaaccccttg
    22381 acaatccgcc gcagattctc gatgtggccg cccggccgga taatccggcg catgcgattg
    22441 aaggggcata tgtgatttac cgcagcggct tttattatct gtttgtgtcg tttgattcct
    22501 gctgcgacgg ggcagaaagc acctacaaag tgatgattgg ccgctcacag cagccgcagg
    22561 gcccgtatga ggattttaag ggacgcccga tgctgcaggg cggcggcaca ctgcttctgg
    22621 ccggccacga caattggcgc ggtcccggac ataactccgt tctgacaacc gagcagggct
    22681 gctggctggt tcaccatacc tatgatacat ttcatctgga cagccagcgc atcattcagg
    22741 tccggccgct ttactgggca caagatggct ggcctgtggc cggcgaaccg ctgggtcccg
    22801 gcaatccgat gaaggcgccc aaaaccgaca tcaaagccgc tcaaatcatc ggaagctggc
    22861 ggctgtcggc cgattacggc gacgagaaaa tcatcgattt tctgccgtcc ggccgggtgg
    22921 cgcatcggaa agaagccaaa tggtcttttg acggcagaca attgactgtg tttttgccgg
    22981 ggattgccgc tgattctgtt acctgctttg ttgagccgtc gggctcatcg tttatcggca
    23041 gggattcgca aggtgtcatc ctgagagcca aacggctgaa tccgtaatcg tgcaaaatgc
    23101 cctgtgtccg ggggcgggca gggctgatgc tgcgcctgaa tgcaggcagg caaaatgctg
    23161 gttgagcggg gggcaatgcc gcctttaagc aggggtatta atccgacagc cattgtccag
    23221 atggtcccaa ttctgcatgc actatcttgc tatcttcaaa aatccg
//
LOCUS       DAOTBO010000035        22959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1847_length_22959_cov_5.651851, whole genome shotgun sequence.
ACCESSION   DAOTBO010000035 DAOTBO010000000
VERSION     DAOTBO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 22959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22959
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1847_length_22959_cov_5.651851"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PLE88_05895"
     CDS             complement(<1..1123)
                     /locus_tag="PLE88_05895"
                     /inference="COORDINATES: protein motif:HMM:NF018444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76030.1"
                     /translation="MIAFAPQDGLDADALLGPDGLIARQWPAFESRPQQVTMARRIQQ
                     AMREGFHLAAEAGTGVGKSFAYLAAAIDQAVRQTGKVLISTYTLNLQQQLIEKDIPFL
                     EEIIPGSFTARLAKGRGHYLCRRRLEYALRHQQTLFDDTAQLAALAAWAAETSDGSLS
                     DLPSAPSADVWQLVQSEHGNCRGRKCFYFSKCFYWRARRTLETADIIVANHALLFSDL
                     ALKEAGVGILPEYTYVVVDEAHNLEHVAEEYFGIRISQYAVVSLLDRLYHHRKRKGLL
                     AYAKEGSQARTAVQACRRAARLFFGQVQAWYAHSGQDSAARCQPEFVEDNLSPSLRTL
                     RLALANLAKQSRDEDQQYELTRFGDQLNGLETDLKDFVGQ"
     gene            complement(1143..2540)
                     /locus_tag="PLE88_05900"
     CDS             complement(1143..2540)
                     /locus_tag="PLE88_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76031.1"
                     /translation="MKHTAGHCIVVCILFCLSTLCLASDNPNVANPNAPSPSFRARTN
                     PPSAGQPQRFVTRPNAPYETSGNDIVTGNVAGMKYFRGFVPYGSSYYSGAYASDRGAS
                     SVSDFLRRSAEPTIADRNPGRIASYYDPQRAVSSFQRQTGIASFAPPSLARQMSDNLV
                     PLESLPMQTPFRPRPLSSNNQELELLLSRQAELEKAADLDAAQRQQEPYKFKSIFDQT
                     LTPQEIQVLKDAAQKTEPQNKSKEEAKTPEQQPETADQVTADERPESAARQVLDEISS
                     KVNRDLEAALKSINPDEGRALLGEHKTFESLAQAKFDGYIKTAETFLSQGQFYKAADT
                     FALAAVWKPADARAYLGQAFSLFAAGEYMSSAYYLSRAIELDATTASRHYDLPAITGG
                     RDVYESRLTELTSWQQRSGSGELAMLMAYVYWLDGKLQQAAGAIRIAADTMAESSATA
                     VLKKLILPNESAGQP"
     gene            complement(2591..3457)
                     /locus_tag="PLE88_05905"
     CDS             complement(2591..3457)
                     /locus_tag="PLE88_05905"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOM76032.1"
                     /translation="MKKPKFILFADPSRPHAVEAMERFTAFAGGKIIVLSNCLESVCP
                     LETLQQADFAVVFGGDGTILGAARQLCQTGVPVIGVNVGKLGFLAEFSIEELQRDFER
                     ILMGQALIEKRMILHCQVLQQGQPVFESTAVNDAVITAGPPFNMIELELSVQGQKLAL
                     CIGDGVIVSTPTGTTAYNLSAGGPILSANLAAVVITPICPHSLSFRPIVINAENQVRI
                     VPMRLNEQTTLLLDGQVQRKLSVGQAVLLQKHPGQFRVVNNPRRTQWETLASKLNWAE
                     KPRYSETSDKLS"
     gene            complement(3472..5388)
                     /locus_tag="PLE88_05910"
     CDS             complement(3472..5388)
                     /locus_tag="PLE88_05910"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOM76033.1"
                     /translation="MLPQKLQNILRAVLWGVISTAGYCQPLYYTEASAAKLTAEARQR
                     IEAIAALLENHPQGFGMPVEKRDFWEQLGNTEAGQNILRKAESLIQRPMPLLTDELYL
                     MYSQNGNRSAAEDVIFARQDRLAALCLAECIENKGRFIGSLEEVLNSCVSFRSWVYPA
                     HDGNLDNFYQRAITIDLKAAHDAHNFALCLYLLGSKINPQLVEKTRTQLHERIFAPYC
                     RDLFEGQRSCWWKTATHNWNPVCHSGVVGAAITLLPEKFDRALFIERAIHYMDYYYQG
                     FTPDGYCSEGLGYWNYGYGQYIVLSEAIYSATKGQIDLFSNPKGLAAAMYPFRIEILN
                     GVYPAIADCSVNIRPQAEWMGYVNARLGLGDARWPVIRPAASNRSLVEFLMFCRMDLN
                     RPVLTPPFMPDNGGPRTWFADAGVLLCRPSNSGSLAAVFKGGHNAEHHNHNDLGSFIV
                     VVGDQPVIVDPGSVEYTRTTFGKDRYTIPKLRSYGHSVPRIGGQEQIAGRDAEAKILH
                     TDFNETTDTVVMDLASAYPVQGLRKLQRTFIYHRSPIESLEVIDEFALTDSNTYEIAL
                     ITYGTCKQTGSDRLEITSKNRTLEVRLENTDNNIKISDEVVKSTRGDFRRIAIQFADP
                     VKIGKISMRISPKL"
     gene            complement(5412..7328)
                     /gene="dxs"
                     /locus_tag="PLE88_05915"
     CDS             complement(5412..7328)
                     /gene="dxs"
                     /locus_tag="PLE88_05915"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOM76034.1"
                     /translation="MSTLLETIQGPQDLRRLSMEQLNHLAAEIRELITQTVSQTGGHL
                     ASNLGIVDLTIAMHRVFNFRRDRLLWDVGHQCYAHKILTGRKELFGRLRQENGLSGFP
                     SPEESEYDVFSVGHAGTSIATALGMALGAMHNGTDEKIVALVGDASIVNGLSFEALNN
                     VSLVKRQMLIVLNDNSMAIDVTQGAMAAFLSRVRLSHTYEDIRKTTNRILEHLPLVGR
                     PMEEALENLKKTLRMANTPSRLFESMNIAYFGPVDGHDIGSLIDLFEALSELNTPAIL
                     HVYTRKGKGYLPADDNPRKYHSTGPFEVNGSGSQAESEQRTFTSAFGEALVEMAAGDR
                     RIVAITAAMPDGTGLVPFREKFPQRYYDVGIAESAAVDIAAGLAKQGLRPVVCIYSTF
                     LQRSFDQIFQEASLQNLPVIFCVDRAGAVGSDGPTHHGFLDVGCLRMLPNLTVAAPAD
                     EMELKAALQAAVAGNGPFAVRYPRDAVQSIPENNAPYQVGKSAVVRQGDSDFVVVTLG
                     PILQEALQAADMLEKEGLSITVVNARFAKPIDSSLEVWFEQGKTVIFAEDSNRACGFG
                     SAVIERMLQTAGDNSPIRQAVGKAILLGAPDGFLPVASRRRQLEWMGLTAAQLARTIL
                     TLSGSAAKTAETSI"
     gene            7557..8753
                     /locus_tag="PLE88_05920"
     CDS             7557..8753
                     /locus_tag="PLE88_05920"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOM76035.1"
                     /translation="MANAVVSQSGGPTGVINASLVGVIEEAAKHKEIDRLYGGLHAVA
                     GIVKEQFIDLKKLSVDTLEKVAASPSSALGSSRDKPDAAYCEKILNVFKKRDVRYFYY
                     IGGNDSANTCYIINTMAEKTGYDLKAFHVPKTVDNDLLVTDHCPGYGTAAKFVACALM
                     GDDLDNRALPGVKIDVIMGRHAGFLTAAAVLAKQREDDGPHLIYIPERPVTMDKFLAD
                     VDAVYKKLGRCVVAVSEGICDHDHITWAEKLAQNAERDAHGNVQLSGTGALADFLAAQ
                     IKGKLKIKRVRADTFGYLQRSFAGLQSETDVKEARACGRMAVLYSLKEDNGSVAMKRT
                     GNGAKYGVEYFRTELSSVAEKTKSLPDAYINADGNGMTEAFVEYAMPLAGTLPQTEYL
                     GNYPLV"
     gene            complement(8843..9364)
                     /locus_tag="PLE88_05925"
     CDS             complement(8843..9364)
                     /locus_tag="PLE88_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76036.1"
                     /translation="MKKKTAFTIIEVLMALVILAMLTTAAALAFDASVKNYQTNQGIY
                     QTVNTARAALLRITNDIRTANAVALIADEPATQVSLVTRDGKNITYRFNADDSTLYYD
                     DNSTGQSYALCGHVAAMTFSRTECQVERDNGAGGMTTFTGIRDVRIVMTVTDDAGKVS
                     KTLAAAALVRRNL"
     gene            complement(9375..9809)
                     /locus_tag="PLE88_05930"
     CDS             complement(9375..9809)
                     /locus_tag="PLE88_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76037.1"
                     /translation="MKTYRQYRGFTLLEALFAAMLIGLVVVALVAASGAFTVANGYGV
                     DLSTAEFLIEQVREMTTRPEVTIDGLISLDDVAWGPPKDAAGNTLPEFSEFIQKIDAD
                     YLQPGNLTQVSSSPTDFIRLTVTIYKNNVPISSTSWVRARLN"
     gene            complement(9910..11079)
                     /locus_tag="PLE88_05935"
     CDS             complement(9910..11079)
                     /locus_tag="PLE88_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM76038.1"
                     /translation="MSLLEKLKPQDTKAKGLQRSQHVAAAIASTSAGVAHKGGFHIDF
                     GPSKKDILNFTNQLAVMVRAGISLQDALESIGTQIAKEKFRYVVLDLKARIEGGQSFS
                     QALAAHPEVFSNLYINMVAAAEISGSMSSMLQRLTEYLDQEAETRSQVISAMVYPGII
                     ATMAIVCVTFLLTFVLPRFLVVFAGKEHLLPGPTKVIMALSMVMRNYWFLVFPGIGAA
                     LMGFWYFTSKTTVGKYWWDKTKLRLPLLKTLCRSLYITRGLHTMGVLTNAGVPILDTL
                     SITAHISGNVLFEDMWKGVFEEVRQGKKIAPSLGNYALMPANVVQMIQSGEESGTLGD
                     VLRDISEFYARELKTVIKTVTSMIEPLMIVVMGVLVGFIAMSIILPIFKMSSVVG"
     gene            complement(11098..12186)
                     /locus_tag="PLE88_05940"
     CDS             complement(11098..12186)
                     /locus_tag="PLE88_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOM76039.1"
                     /translation="MIDTKQMLAAAIKNSASDVHINVGMPPIMRINTELIMMDLPEVS
                     NEDAGRMILEMIGEDKFKLFLEKRDWDFSTMIEDGSRFRVNAHFQKETFALSFRAISN
                     SIPDIEDLHLPEVVKELTDLPRGLVLVTGHTGSGKSTTLASMINEINRKYRKRIITLE
                     DPVEYLMENKSCMIEQREIGSDCPSFASGLRHALRQDPDIILVGEMRDLETTSSTITA
                     AETGHLVLSTLHTINASQTVERIIDVYPPSQQNQIRAMLANTLQAVISQTLFRRVDQP
                     GMVPCTEILLCTSAVRNCIRENRIYEIPNIIETSRRLGMQTMDHSIQQMYIKGYIDRD
                     EAIIRATNPGKMEKILPATPQGALQEQV"
     gene            complement(12233..14089)
                     /locus_tag="PLE88_05945"
     CDS             complement(12233..14089)
                     /locus_tag="PLE88_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOM76040.1"
                     /translation="MKRIFGKKVQPQAAALLELDSGQTDLAPGLQNLYSPQITTQTRL
                     RDIADILAEDEILTLGALDQIRQDQQAGGELEKLLAERGITAEQILAAKAKLHGYAFE
                     RIAPQDVDKKAFELLEISAIRSQQIMPVRFREDGTLVVATTQPSNVFAIDDVKRQTGK
                     SVDVVVCPAEDIEAVCSEFDESKFDCSVEEFAMEDIELVQDTEEEVEDLEKSAGQSPV
                     IKFVNFLLSNALHEGASDIHIEPKEKYTKIRYRIDGILFDIKQAPSRMHPAIVSRLKI
                     MANLDISERRVPQDGKIAVIMGGRGVDLRVSVLPTCHGEKVVIRLLDSKSIMISLEKS
                     GMQPRILKAFQEQIALPHGILLVTGPTGSGKSTTLYSALAQMDSDRLNVSTVEDPVEY
                     NLEFCNQVQVNEKAGLTFAAALRSLLRQDPDIIMVGEIRDQETARIAVQAALTGHLVL
                     STLHTNDAPSSVSRLVNIGIEPYLIAASLNGILAQRLVRRICNNCKEPFAAPEGLQKY
                     LDIAGIEPHELMHGRGCDQCRNSGYAGRCGIHELLIVDDEFRRIINTDSSVNSMREAF
                     RRSGWPNLFEDGLQKVKQGITTIEEVLRVAEVADALDPAAAGEKKQGAAVAV"
     gene            complement(14086..14742)
                     /locus_tag="PLE88_05950"
     CDS             complement(14086..14742)
                     /locus_tag="PLE88_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76041.1"
                     /translation="MLNYLKDGSQSPVLEQAQQAAQSPDTASRPADYLTVSGHGRKVR
                     QSTILLIAVFAVGALAVWFMIKKTAPASAEAAASQDQTQLDAALAQLNAMQSEVNSQM
                     NSVVGRFYQFSNIDQVGVDELKKNPFRRELDFAADAPDSDALYAQQQMRLREEANRLQ
                     MGLELWSITATPKGMCCMINDKVLYVGDTFREMTVKSIENKTVLLDYKGVPILLKMEQ
                     "
     gene            complement(14774..15304)
                     /gene="pilO"
                     /locus_tag="PLE88_05955"
     CDS             complement(14774..15304)
                     /gene="pilO"
                     /locus_tag="PLE88_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOM76042.1"
                     /translation="MNSGIRYLIFFVLIVGLSYVAWAYMIKPANAHLTQERAKMEALQ
                     AKLNELERATATAQDLNVQLQKIEEAIAAFESKLPPSSEIHTVLENVTLIAQRQGLTP
                     KAIRTLKNRQNRGYIEQPLSMELHGNFNSYYAFLLELEKLDRITKIRELTLKKSKQEG
                     QTEATFVMSIFFQSAA"
     gene            complement(15339..16049)
                     /locus_tag="PLE88_05960"
     CDS             complement(15339..16049)
                     /locus_tag="PLE88_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOM76043.1"
                     /translation="MATINFVPDDYVQQRRASRANILYLMLLMAMLGAIGITFGFIKM
                     RQRAVQAELAQLNERMAKAQQQIAQLEELKAKSKTMMKTMVMTAELLEPVPRSVILAS
                     LTNNLPSGVSLTEFKLEEKEVQTARPSPAKPAGSNQYQRAAATAKSEPTDQPQVRIET
                     NLDIKGLAPSDIQVAGFIARLSSSILFDAVSLVESKEVEIDKIRMREFRLKAALKPGL
                     TLTKEDIDSIREKRNQLM"
     gene            complement(16068..17045)
                     /gene="pilM"
                     /locus_tag="PLE88_05965"
     CDS             complement(16068..17045)
                     /gene="pilM"
                     /locus_tag="PLE88_05965"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOM76044.1"
                     /translation="MVLGFNSKKTCPIGVDMGSGYLRMVQIGRNGNGLYLHSATLRAV
                     PADIEFRSPAWQHWAVDTVRSLMREGQFKGKEVITALPTDDLFIDPVKVPRTTVDRLD
                     EVIPPKLQKRLPFPVNDALFQYIVAEPTERAPSDSADILTIAVNREAVNRHLAIYEAA
                     GLEVSKIGIWPTAMITSFSKFFCRRSNEQNVIALLLTVGTSHSNVVITRGANLLFARA
                     ISIGYAQLEQTQMLQRLLSELDACVRYFEESFSGARIERLVFLAGSGASRTLCDRFAE
                     LARRMQIPAQVGDVTSAIEINQTPQCLIDRRNSRVDWATAFGLSLEGLS"
     gene            complement(17530..19146)
                     /gene="nadB"
                     /locus_tag="PLE88_05970"
     CDS             complement(17530..19146)
                     /gene="nadB"
                     /locus_tag="PLE88_05970"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOM76045.1"
                     /translation="MPTRQADIRRYLVPINTASTQQLFTDCLVIGAGIAGLRAALEAA
                     PHCNVTLVCKGTFSDTNTWNAQGGIASVLDEVEDSFEAHIEDTLRTGCGLGDKPVIER
                     VVREGPGLVRQLADWGTAFDLVDGEIDISLEGGHSHARIAHSHGDSTGRGIAETLIAR
                     VQQSPTINVMEHFFTIDLLTSEDGQCVGVIGRRPSGSLQIIWAGATILATGGAGRLYR
                     ETTNPECATADGLAMAWRAGAVLRDMEFMQFHPTTLYIAGASRALVTETLRGEGAILR
                     DINHEPFMKEYDPRGELAPRDVVSRAILDRMLKTQATHVYLDIRHFDKAHFKKRFPLI
                     SELCESFDIDVSTDLIPVRPSAHYMIGGLKADISAATSIPRLFACGEAASTGLHGANR
                     LGSNSLLEGLVFGRIAGQSAVELLAKAGTVGYRRMQFDVPPSDRSRLDTDDVRNSLRA
                     LMWRNVGITRSATPLQEAMEIIQFWQRYVMDKVFSEPFGWECQNMLTVALLMAHAAYK
                     RQESRGVHYRQDYPNRDDKHFSYHIELARE"
     gene            complement(19157..19894)
                     /gene="bioD"
                     /locus_tag="PLE88_05975"
     CDS             complement(19157..19894)
                     /gene="bioD"
                     /locus_tag="PLE88_05975"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOM76046.1"
                     /translation="MLELRLPKKNGLFITGTDTGVGKTLITGAIAAVLRRQGLKVGVF
                     KPIATGCRPSREGLVSTDAEFLALCADADYPLSVITPVTYKTPAAPLVCAEIEGRPID
                     FDAIAAAYKYLCDTCDIVLVEGIGGILVPMTPTETVLDLAVEFDLPAVIAARPNLGTI
                     NHSLLTIQAARNAGLPVAGLVISGYNAARADTAEETAPDVICSISRVNLLAVVPFDED
                     SSVENGKLSPLIIDTLSLCDWNALSRA"
     gene            complement(19940..22000)
                     /locus_tag="PLE88_05980"
     CDS             complement(19940..22000)
                     /locus_tag="PLE88_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM76047.1"
                     /translation="MLYKQLAYVISFLVAVGLLSAEEIHLLKPVPAGSVRIEDAFWSP
                     KLNIWNSITLKNVLDKFEAAGAFRNFDRIAGKLEPKHEGPPWFDVLVYETIRGASDFL
                     VAYPDKELEARIDGYIQRIAAAQAADPDGYVMTYTQLEEPDHRWGLNGGFLRWQHDVY
                     NAGALVEAGIHYYRATGKIDLLRAAVKLANYMTQIIGPEPKKNIVPAHSLAEEAMMKL
                     YVLFQESPSLKERLNVPVDEAAYLHLAEFWIEMRGRHCGQPASEMWDRNGRGCEEWIR
                     QQKYGSGRPSWGEYAQDHKPVFQQETMEGHAVRATLLCTGLSAAAAVNGKTAYADAAV
                     RLWENMVGKRMHITGGVGAFAHEEKFGPDYTLPNDAYLETCAAVGAGFFHFNMNLLFA
                     DARYIDQLERTLYNNVINGVSLDGTRYYYQNPLSANGHRRWQWHDCPCCPPMFLKFVS
                     GLGGYIYAYDSQEIYVNLFIGSKAVIPLENQSVKIVQQTEYPWKGNVKIVLDTQAQTE
                     FALFVRIPGWARGQENPFGLYTSDLASQFELRVNGQSISASEISRGYAVIKRAWNKGD
                     EVILELPVQPRRIYAHPEVKACRGRLAIQSGPLVYCIEQQDNPAIDTISLKTGSPLVL
                     EYNPDFLGGVNCIKAEAFSIGPNGQAQNVTLTAIPFYCQDNRGNTARMEVWLPDEKRK
                     SVSN"
     gene            22168..>22959
                     /locus_tag="PLE88_05985"
     CDS             22168..>22959
                     /locus_tag="PLE88_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM76048.1"
                     /translation="MKQYVTIITLGIVLWAAPLLEAMDNPFSYEDGINDCDVIKYNGE
                     YYITGNWLGGDMFRSRNLTDWGERIHIFSWNNTWHVQRNANRDMDIHGTHIAYDNGQF
                     HLYAHLDTPAGNLLGIVHAVSNQVTGPYTEPVDAPFATDTIDVKTFRDEDGSLHFYST
                     RFGGVSANHNDYRSMQDYSTFTSGYKTLIWPTGGWEIHPELPEFSYGPTINEGPFVFK
                     YRGKYYMLYNANHTGDTSYAIGCAMADTASSFSNAGKLPSPVLGKT"
BASE COUNT         5564 a         6122 c         5622 g         5651 t
ORIGIN      
        1 gctgtccgac aaaatctttc aaatccgttt ccaaaccgtt gagctggtcg ccgaaacgtg
       61 tcagctcata ttgctggtct tcatctcggc tctgttttgc cagattggcc agagcaagcc
      121 gcagggtccg aagggaagga ctcaggttgt cctctacaaa ttccggctga cagcgtgcgg
      181 cgctgtcctg accgctgtga gcataccaag cctgcacctg tccgaaaaag agccgggccg
      241 cccgacggca ggcctgcacc gcggtgcggg cctgactgcc ctctttggca taagccagca
      301 gccctttgcg cttacggtga tgataaagcc ggtccagcaa actaacaacg gcatattgac
      361 tgatgcggat gccgaagtac tcttcggcaa catgctcaag attgtgggct tcgtcaacca
      421 cgacataggt atactccgga agaatcccga cgccggcttc tttgagggct aaatcactga
      481 acaggagtgc atggttggcc acaataatat cggccgtttc caatgtgcgc cgggcacgcc
      541 aataaaaaca tttggaaaag tagaagcact tgcgtcctcg gcagtttcca tgttcgctct
      601 gaaccaactg ccagacatca gccgaaggag cagagggaag gtctgacagc gaaccgtcgg
      661 aggtttctgc tgcccaagca gccaaggctg ccaattgagc agtatcatca aacagggtct
      721 gctggtgtct gagggcatac tccagccgcc gcctgcaaag ataatgaccg cggcctttgg
      781 ctaatcgggc ggtaaaactg ccgggtataa tttcttccag aaagggtata tctttttcaa
      841 taagctgctg ctgaaggttg agtgtatagg tgctgataag cactttgcca gtctggcgaa
      901 cagcctgatc aatggcggcg gccagataag caaagctctt gcccactccc gtaccggctt
      961 cagcggccag atgaaaaccc tcgcgcatgg cctgctgaat tcggcgtgcc attgtcacct
     1021 gctgggggcg tgactcaaaa gccggccatt gacgggctat cagaccgtca ggacccagca
     1081 gtgcatcggc atccaaacca tcctgtggcg caaaagcaat caccgccgcc tccgtatttt
     1141 actcacggct ggccggccga ttcatttggg aggataagct tctttaatac cgctgtcgct
     1201 gaactttcag ccattgtatc agcagcaata cgaatagccc cggcggcctg ctgcagtttg
     1261 ccgtccagcc aatagacata ggccatcagc atcgccaatt cgccagagcc gctgcgctgc
     1321 tgccagctgg tcagctcggt cagacggctt tcatatacat cacgcccgcc agtaattgcc
     1381 ggcaagtcat aatggcggct ggccgttgtc gcatccaatt ctattgcgcg gcttaaataa
     1441 taggcactgc tcatatattc gccggcagca aacagtgaaa acgcctgtcc cagataagcc
     1501 cgggcatccg cgggcttcca tacagccgcc agtgcaaatg tatcggcggc cttatagaat
     1561 tgcccctgcg acagaaatgt ctcagctgtt ttgatatacc cgtcaaattt tgcctgtgcc
     1621 aacgactcaa atgttttatg ctcgcctaaa agcgcccggc cttcgtctgg atttatcgat
     1681 ttcagcgccg cctcaaggtc ccgattgact ttgctgctga tttcatcgag cacttgccga
     1741 gcagcagatt cagggcgctc atccgccgtg acttggtcgg cggtttcagg ctgctgttca
     1801 ggagtttttg cttcttcttt ggacttattc tgcggttcgg ttttctgtgc cgcatctttg
     1861 agcacctgaa tttcctgcgg tgttagggtt tgatcaaaga tgcttttgaa tttgtagggt
     1921 tcctgctgtc gctgtgcggc atcaaggtca gcggcctttt ccagttcggc ctgccggcta
     1981 agcagcagtt ccaactcctg attgttggat gacagcggcc gcgggcggaa aggtgtctgc
     2041 atcggcaagg attcaagggg tacaagattg tccgacatct ggcgcgccag tgacggcggg
     2101 gcaaaagacg cgatgccagt ttgtctttga aacgatgaaa ccgccctctg ggggtcataa
     2161 tagctggcta tgcggccggg attgcggtcg gctatcgtcg gctcagccga gcgcctcaaa
     2221 aagtcgctga cagaactggc gccccgatca gaggcatagg caccgctgta atagctggag
     2281 ccatagggga caaagccgcg gaaatacttc atacctgcca cattgcctgt aacaatatcg
     2341 ttgccgctgg tttcgtaagg agcattggga cgtgtaacaa accgctgcgg ctgaccggca
     2401 ctggggggat tggtgcgtgc ccgaaaagac gggcttggtg cattgggatt ggccacattg
     2461 ggattatctg aagccaaaca tagagtcgaa agacaaaaca gaatacagac aactatgcaa
     2521 tgacccgctg tgtgtttcat atggcacttt cttttgaaca tatcaaaccc tcttttataa
     2581 tttcaacaaa ttaggatagt ttatcagatg tctcactgta tcggggtttt tctgcccagt
     2641 tgagtttgct tgccagtgtt tcccattggg tccgccgcgg attgttgact acgcggaatt
     2701 gtccggggtg cttttggagc agtaccgcct gccccacaga caacttgcgc tgcacctgac
     2761 catccaacag caaagttgtc tgttcattga gccgcatagg cacaattcgg acttgatttt
     2821 cagcattgat gactatcggc cgaaaactta acgaatgcgg acagatcggg gtaatcacta
     2881 ctgccgccag attggctgac agaatcggtc cgcccgctga cagattatac gccgtcgtgc
     2941 cggtaggcgt tgaaacgatt acaccgtcgc cgatgcacaa cgcaagtttc tgcccctgga
     3001 cggataattc cagctctatc atattaaacg gcggacctgc tgtaatcaca gcatcattca
     3061 cagcggtgga ttcaaaaacc ggctgaccct gctggagcac ttggcaatgc agaatcattc
     3121 gtttttcaat gagcgcctga cccattaaaa tacgttcaaa atcacgctgg agttcttcga
     3181 tgctgaattc ggccagaaag cccaatttgc caacattaac tccaatgacc ggtacaccgg
     3241 tttgacaaag ctgacgagct gcacccagaa ttgtcccgtc acccccgaaa acaaccgcaa
     3301 aatctgcctg ctggagagtc tccaacggac agacgctttc caaacaattc gacaagacta
     3361 ttatcttccc gccggcaaag gcagtaaagc gttccatcgc ctctaccgca tgcggtcggc
     3421 tcgggtcagc aaacaagata aatttcggct ttttcatagg gattttaata tctataattt
     3481 cggcgaaatc cgcattgata ttttcccaat tttgactgga tcggcaaatt gtattgctat
     3541 ccgccggaaa tcaccacgcg tggattttac tacttcatcg ctgattttaa tattattatc
     3601 ggtgttttcg agccggactt ccagagtcct gtttttggag gtaatctcca gccggtctga
     3661 acctgtctgt ttacaggtgc cataagtaat caaagcgatc tcatatgtgt tggaatctgt
     3721 caatgcaaac tcgtcgatta cctccagtga ttctatgggg cttctatgat agatgaaggt
     3781 acgttgaagt tttcttagcc cctgaaccgg ataagcactg gcaagatcca ttacaaccgt
     3841 atcggtcgtt tcattaaagt ccgtatggag aattttggct tcagcatccc tgccggcgat
     3901 ttgctcttgt ccgccgatcc gcggtacaga atggccatac gaacggagtt tgggtatcgt
     3961 gtaacggtct ttgccgaatg tggtacgggt atattctaca gaacccggat ccacaataac
     4021 cggctgatca cccaccacta caataaagga acctaaatca ttgtggttat gatgttcagc
     4081 attatggccg cccttaaata cggcagccaa agagccgctg ttggatgggc ggcaaagtag
     4141 cacccctgca tccgcaaacc aagttcgggg gccgccgtta tcgggcataa acggcggagt
     4201 caaaaccgga cggttcaaat ccattcggca aaacatcaga aattccacca gcgaacggtt
     4261 tgacgccgcc ggtcggataa caggccagcg tgcatcgccc agtcccagcc gagcattgac
     4321 atagcccatc cactctgctt gaggccgaat atttacgctg cagtcggcta ttgccggata
     4381 tacgccattg agaatttcaa tacggaatgg atacatcgca gcagccagcc ccttaggatt
     4441 gctgaacagg tctatctggc ccttcgtggc tgaataaatc gcctcgctca ggacaatata
     4501 ctgtccatat ccgtaattcc agtagcccag tccttccgag cagtatccat ccggcgtaaa
     4561 accctgataa taataatcca tataatgaat tgcccgctca ataaacaagg cacggtcgaa
     4621 tttttccggc agcagtgtta tagccgctcc tactacgccg gaatgacaga ccggattcca
     4681 attatgggta gctgttttcc accagcagct gcgttggcct tcaaaaagat ctcggcaata
     4741 gggagcaaaa atccgttcat ggagctgtgt gcgggttttc tcaaccagct gcggattgat
     4801 tttgctgccc agcagataca gacacagcgc aaagttgtgt gcatcatgag cagcttttag
     4861 gtcaatagtg atagcacgct gataaaaatt atccagattg ccgtcatgag caggatatac
     4921 ccacgagcgg aagctcacgc aggagtttag cacctcctcc agcgatccga taaaacgtcc
     4981 tttgttttcg atgcactcag ccagacacag cgctgccagc cggtcttggc gtgcaaatat
     5041 tacatcttct gcagcagaac ggttgccgtt ttgggaatac atcaaataca attcatccgt
     5101 tagcaaaggc atcggccgct ggataagcga ttctgccttg cggagaatat tctgtccggc
     5161 ttcagtattg cccagctgct cccagaaatc ccgcttttcg accggcatgc caaatccctg
     5221 cgggtggttt tccagcaatg cagcaattgc ttcaatccgc tggcgcgctt ccgccgttaa
     5281 tttggctgcg gacgcttcgg tataatataa cggctgacaa tagccggccg tactgattac
     5341 tccccataat acagccctta aaatattttg gagtttttgg ggaagcattg attatcctcc
     5401 tcttccgcta cttaaatact tgtttctgct gttttggcgg cagaaccgga tagtgtcaat
     5461 atagttcgtg ccagctgggc agcagtcagc cccatccact caagctgccg gcggcgcgat
     5521 gcaaccggca gaaaaccgtc aggagccccc agcaggatgg ctttgccgac ggcctgccgg
     5581 attgggctgt tgtcccctgc tgtttgcaac atccgctcaa ttaccgccga accaaaaccg
     5641 caggccctat tgctgtcttc ggcaaaaatg actgtcttac cctgttcaaa ccacacttcc
     5701 agtgaggaat cgatcggctt agcaaagcgg gcgttaacca ccgtaataga aagcccttct
     5761 ttctccagca tatcggccgc ctgcagcgcc tcctgaagaa taggccccag cgttaccact
     5821 acaaaatctg aatccccctg acggacaacg gcggatttgc cgacttggta tggagcgttg
     5881 ttttcaggaa tagactgcac cgcatcccgc gggtagcgaa ccgcaaacgg tccatttccc
     5941 gcaacggccg cttgcagcgc tgctttcagc tccatctcat cggccggagc cgccacagtc
     6001 agattcggca gcattcgcag acagccgaca tccagaaaac cgtgatgcgt tgggccgtcg
     6061 ctgccgactg caccggcacg gtccacgcag aagatgaccg ggagattctg cagggaggcc
     6121 tcctgaaaaa tctggtcgaa actgcgctgc agaaatgtcg aatagatgca caccaccggc
     6181 ctcaagccct gcttggccag tccggctgca atatcaactg ccgcactttc ggctatgcct
     6241 acatcataat agcgctgggg aaatttctcc cgaaacggca ctaatccggt cccatcaggc
     6301 atagcagccg taatggccac gattctcctg tcgccagcgg ccatttcaac cagagcttca
     6361 ccaaaggccg aagtaaaggt tcgctgctcg ctttcggcct gactgccgct gccgttgacc
     6421 tcaaaaggac ccgtgctgtg gtattttctc ggattgtcgt cggcgggcag atatcctttg
     6481 cccttgcggg tatagacatg gagtatggcc ggagtattca gctccgacaa ggcctcaaat
     6541 aaatcaatca gcgaaccgat gtcgtgtcca tccaccggac caaaataagc aatattcatg
     6601 ctctcaaaca gccggctggg cgtattggcc atgcgcagcg tttttttaag attttccaag
     6661 gcctcttcca tcggccggcc aaccagcggc agatgttcga gtatgcgatt ggtcgtttta
     6721 cggatatctt cataggtatg gcttagccgc acgcgcgata aaaatgccgc cattgctcct
     6781 tgggttacat caatagccat cgaattatcg ttaaggacta tcagcatctg ccgtttgacc
     6841 agcgatacat tattgagcgc ctcaaacgaa agtccattga caatacttgc atcgccgacc
     6901 aaggcaacaa ttttttcatc ggttccgttg tgcattgccc ccagtgccat cccgagggct
     6961 gtggcaatag atgtgccggc atggccgaca ctgaagacat catactcgct ctcctcaggg
     7021 ctgggaaaac cggacaatcc attttcctga cgaagccgac cgaataactc tttgcggcca
     7081 gttagaatct tatgggcata gcattgatgg cctacatccc acagcagccg gtcgcggcgg
     7141 aagttaaata cccgatgcat tgcaatggtc agatccacaa tacccaagtt gctggccaga
     7201 tggccgccgg tctgactgac tgtttgggtt atcagttcgc ggatttcagc ggccaaatga
     7261 tttaattgtt ccattgataa gcggcgcaaa tcctgcggcc cttggattgt ctcaagcagt
     7321 gtactcatcc aacctcaaat tcaggacaat atcgttcgtc aaacaaaaca atgtattata
     7381 atgcttttaa gaaaactttt caaacatctt atattacagc atcgaaggat taattttgaa
     7441 tactgcatct ccaaaatcaa ggtttggcct tgcaaaaaag ggactgttgt gtataatttc
     7501 ctgcccaatg gtattaaaac agtaaacaga aactacctta taagaaaggt tttatcatgg
     7561 ccaatgcagt tgtcagtcaa tcgggggggc caacgggtgt tattaatgcc tcgctggtag
     7621 gcgtcatcga agaagctgcc aaacacaagg aaattgatcg tctatacggc ggcttgcacg
     7681 ctgtagcggg gattgttaaa gagcagttta tcgaccttaa aaaactatcg gttgatacgc
     7741 tggagaaagt tgccgcttcg ccctcgtctg ctttgggttc cagcagggat aagcccgatg
     7801 cggcctattg tgaaaagata ttaaatgtat tcaagaaaag agatgtccgt tatttctatt
     7861 atatcggtgg caatgactcg gctaatacat gctacattat taatacgatg gccgaaaaga
     7921 ccggttatga tctaaaggcc ttccatgttc ctaaaacagt agataatgac ctgctggtaa
     7981 ccgaccattg cccagggtat ggaacagcgg ccaaatttgt ggcttgtgct ttgatgggcg
     8041 atgacttgga caaccgcgct cttcctggcg ttaaaattga tgttattatg ggacggcatg
     8101 ccggtttttt gacagcagca gcagtccttg ccaagcagcg tgaagatgat gggccgcatt
     8161 tgatttatat tccagagcgg cccgtcacca tggataaatt tttagcggat gtggatgctg
     8221 tttataagaa actgggccgc tgtgtggtag cagtcagtga aggcatctgc gaccatgacc
     8281 atataacatg ggcagaaaag cttgcccaaa atgccgaacg ggacgctcac ggcaatgttc
     8341 agctttccgg cacaggggcc cttgccgact tcttggccgc ccaaatcaag ggcaagctca
     8401 aaattaagcg cgttcgggca gacacctttg gttatctgca gcgcagtttt gccggacttc
     8461 agtcagagac cgatgtcaag gaagcccgtg cgtgcggtcg aatggctgtg ctgtattcgc
     8521 tgaaggaaga caacggttcg gtggccatga aacgcacggg aaatggagcc aaatacggcg
     8581 tggaatattt ccgaactgag ttgtccagcg tggccgaaaa aaccaaatct ctgccagatg
     8641 cttatatcaa tgccgacggc aacggaatga cagaggcctt tgttgaatat gcgatgccgc
     8701 tggcaggaac tctgcctcag acagaatatc tggggaatta tccccttgtt tgaaaagtgc
     8761 cggtggtatt aaactggcag actaaaaaat tatgcgggat tgcttcaaaa acccgccgga
     8821 aatcaataac cggcaggttt ttttacaggt tgcgccgtac caatgcggcg gcggcaaggg
     8881 ttttagagac tttgccggca tcatcggtta cagtcatcac gattcggaca tcccgaatgc
     8941 cggtaaaggt agtcataccg ccggcgccat tgtcgcgttc gacttggcat tcggtacgag
     9001 agaaggtcat cgctgctaca tgtccgcaca gggcatagct ttgcccggtg ctattgtcat
     9061 cgtaataaag cgtactgtca tcggcattaa aacgataggt aatatttttg ccgtcgcggg
     9121 taaccagact gacttgtgtg gcaggttcat cagcaataag ggcaacagca ttggctgtgc
     9181 ggatatcgtt tgttatgcgc agcagagcgg cccgcgcggt gttgacggtc tggtaaattc
     9241 cctggttggt ctgataattt ttaaccgacg catcaaaagc caaagcggct gctgttgtca
     9301 gcattgccag aataaccagc gccatcagca cctcaatgat ggtaaatgcg gttttcttct
     9361 tcatacatca ctcgctaatt cagacgagcc cgaacccagc ttgttgagct gataggcaca
     9421 ttgtttttgt agattgttac ggtcaggcga ataaaatcgg ttggcgagga ggagacctgc
     9481 gtcagattgc cgggctgcag ataatctgca tcgattttct gaataaattc cgaaaactcc
     9541 ggcagggtgt tgcctgcggc atctttagga ggcccccaag ccacatcatc cagcgatatc
     9601 aatccgtcaa tggtcacttc cggccgggtg gtcatttctc gcacttgttc aataagaaat
     9661 tcagcagtgg ataagtctac accgtaaccg tttgccacag taaatgcccc gctggcggcg
     9721 accagcgcca ctacaaccag ccctatcagc atagcggcaa acagggcttc cagcagggta
     9781 aaacctctgt attgtctgta tgttttcata atcaatgccc cgttatttgg cgcagattat
     9841 ccctggattc aggaacagcc cgctgtgcac ctgtcccttt gctctgcgga taatctgcgg
     9901 ttttgggttt tatccgacaa cactggacat cttgaaaatg ggcagaataa tgctcattgc
     9961 aataaagccg accagcacac ccattaccac aatcatcagg ggctcaatca tactggtgac
    10021 cgttttaata accgtcttga gctcacgggc atagaattca gaaatatccc gcaggacatc
    10081 acccaaagtt cccgattctt cgccgctttg aatcatctga acaacattgg cgggcatcag
    10141 ggcataattg cccagcgagg gggcgatttt cttgccttgg cgcacttctt caaaaacacc
    10201 tttccacata tcctcaaaca acacattgcc tgatatatgg gctgtaatag acagtgtatc
    10261 cagaatcggc acacccgcat tggtcagcac acccatcgta tggagacccc gtgtgatata
    10321 cagactgcgg cacagtgttt taagaagcgg aagacgcagt ttggttttgt cccaccagta
    10381 ttttccgact gttgtcttgc tggtaaaata ccaaaagccc atcagtgcag caccaatgcc
    10441 cggaaatacg agaaaccagt aattgcgcat caccatactc agggccataa tcactttggt
    10501 tggccccggc aggaggtgtt ccttgccggc aaacaccacc aaaaatcgag gcagcacaaa
    10561 tgtcagaaga aaggttacgc agacgattgc catcgttgca ataattccgg gatagaccat
    10621 tgcactgata acctgactgc gggtttccgc ttcctgatca aggtattccg tgaggcgctg
    10681 gagcattgaa ctcattgaac cggagatttc tgccgccgcc accatattga tatacaggtt
    10741 tgaaaaaacc tccggatggg cggccagggc ctgtgaaaag ctttgtccgc cttcgatgcg
    10801 tgcctttaaa tccaaaacaa catagcggaa cttttctttg gcaatctggg tgccgataga
    10861 ctcgagggca tcctgcagac tgatgcccgc ccgaaccata acagccagct ggttggtaaa
    10921 attcagaatg tctttcttgc tcggcccaaa atcgatgtga aatccgcctt tgtgtgcaac
    10981 gccggctgaa gtcgaggcaa ttgcagccgc cacatgctgg ctgcgctgga gtcctttggc
    11041 tttggtgtcc tgcggtttga gcttttcaag caatgacata gagcgtctcc cttagcatca
    11101 gacctgttcc tgcagagcgc cttgcggtgt tgcaggaaga attttttcca ttttgccggg
    11161 gttcgtcgca cgaataatag cttcatcccg gtcaatatat cccttgatgt acatctgctg
    11221 gatgctgtga tccatcgtct gcatacccaa gcggcgtgag gtttcaatga tattgggaat
    11281 ttcataaatc cggttttcac ggatgcagtt gcggacggcg gaggtgcaca gcaggatttc
    11341 tgtgcagggg accatgccgg gctgatcaac gcggcggaac agcgtctggg aaataaccgc
    11401 ctgcagggta ttggccagca tcgcccgaat ctgattttgc tggctgggcg gatacacgtc
    11461 gatgatacgc tcgaccgtct gcgaggcgtt gatggtatgc agggtactga gcaccagatg
    11521 gccggtctcg gctgcagtga ttgtagaact ggtcgtttca aggtcgcgca tttcgccgac
    11581 cagaataata tccgggtcct gacgcagcgc atggcgaagg ccggaggcaa aagacggaca
    11641 gtccgagccg atttcccgct gctcaatcat acacgatttg ttttccatca gatattcgac
    11701 agggtcttcc agcgtaataa tacgcttgcg gtacttgcga ttgatttcat taatcatcga
    11761 tgccagcgtg gtgcttttgc ctgaaccggt atgccctgtc accagcacca gtccgcgggg
    11821 cagatcggtc agctccttaa ccacctcggg caggtgcagg tcttcgatat ccggtatgct
    11881 gttggagatg gcacgaaatg agagggcaaa ggtttccttc tgaaaatgag cattaacgcg
    11941 gaaccggctg ccgtcctcaa tcattgtcga aaaatcccag tcgcgctttt ccagaaagag
    12001 tttgaattta tcctcgccta tcatttcgag aatcatccgg ccggcatcct cattggacac
    12061 ttcgggcaga tccatcataa tcagttctgt gttgatgcgc ataatgggag gcatgcccac
    12121 attgatatgc acgtcggaag cactgttctt gatagcagct gccagcattt gttttgtatc
    12181 aatcataatt caactcctca ataatctgtg cctttccata cacccccggc tctcacaccg
    12241 caacagcagc gccctgtttt ttttcgccgg ccgccgcagg gtccagggca tccgctactt
    12301 cggccacgcg gagcacctcc tcaatcgtcg taatgccctg cttgactttc tgcagtccgt
    12361 cttcaaacag attcggccac ccgctccggc ggaaggcctc acgcatactg ttgaccgaag
    12421 aatcggtatt gataatgcgg cgaaactcat catcgacaat cagcagttcg tgaatgccgc
    12481 aacggcctgc ataaccgctg tttcggcact ggtcgcaacc cctgccgtgc atcaattcgt
    12541 ggggctcaat gccggctata tcgagatatt tctgcaatcc ttcgggggcc gcaaacggct
    12601 ctttgcagtt attgcagatg cgccgcacaa gccgctgggc taaaatgccg ttcagcgagg
    12661 cggcaatcag atagggctcg atgccaatat taaccagccg tgaaacactg ctgggggcat
    12721 cattggtatg cagcgtgctg agcactaagt ggccggttaa agcggcctgg acggctatgc
    12781 gagctgtttc ctggtcacgg atttcgccca ccataataat gtccggatcc tgacgcaaaa
    12841 gagaccgcag ggctgccgca aacgtcaggc cggctttttc gttgacctga acctgattgc
    12901 agaattcaag attatactct accggatctt cgaccgtgga cacattcagc cggtcactgt
    12961 ccatctgggc cagtgctgaa tacagggtgg tgcttttgcc cgaaccggtg ggaccagtaa
    13021 ccagcaggat gccatgcggt agagcaatct gttcctgaaa cgcttttagg atgcggggct
    13081 gcatccccga cttttccaga ctaatcatga tgcttttgct gtccagcaga cgaatcacaa
    13141 ctttttcgcc atggcatgtc ggcagcacgg acacgcgcaa gtctactccg cggccgccca
    13201 taatcacagc aatcttgcca tcctgcggca cccggcgctc ggagatatcc agattggcca
    13261 taattttcaa acgcgagaca attgcaggat gcatgcgcga cggagcctgt ttgatatcaa
    13321 acagaatacc gtcaatgcgg tagcgtatct tggtgtattt ctctttgggt tcgatatgga
    13381 tatcactggc tccttcgtgc agagcattgc tcagcagaaa attcacaaac ttgataaccg
    13441 gcgactggcc ggcactcttt tcgaggtcct caacttcttc ttcggtatcc tgcaccagct
    13501 cgatatcttc catcgcgaat tcctcgacgc tgcagtcaaa tttgctctcg tcgaattcgc
    13561 tgcagacagc ctcgatatct tcggccggac acactaccac atctaccgat ttacccgtct
    13621 gccttttgac atcatcgatc gcgaaaacat tggacggctg ggttgttgcc actaccaatg
    13681 ttccgtcttc tcggaagcgg accggcataa tctgctgact tcggatggca ctgatttcca
    13741 gcagttcaaa cgcctttttg tccacatcct gcggtgcaat ccgctcaaag gcataaccgt
    13801 gcagtttagc tttcgcagct aagatttgct cggcggtaat gccgcgctct gccagcagtt
    13861 tctccagttc gccgccggcc tgctggtctt gacgaatctg atccagcgca cccagcgtca
    13921 gaatctcatc ttctgccaga atatcggcaa tatcccgcag acgggtttgg gtcgttatct
    13981 gcgggctgta caaattctgc aagcccggcg ccaaatcagt ctgtccggaa tccagctcca
    14041 gcagagccgc agcttgcggc tgcacttttt ttccaaaaat acgcttcatt gttccatctt
    14101 caataaaata ggcacgcctt tataatccag cagaactgtc ttgttctcta tggatttgac
    14161 ggtcatctca cgaaaggtgt cgccgacata cagcacctta tcattaatca tacagcacat
    14221 tcctttggga gttgcggtaa tgctccacag ttccagaccc atctggagac ggttggcttc
    14281 ctcgcgcagg cgcatctgct gctgagcata caacgcatcg gaatcgggcg catcagccgc
    14341 aaaatccagt tctcgccgga agggattctt tttcagctca tccacaccaa cctgatcaat
    14401 gttggaaaac tggtaaaacc gcccaaccac cgaattcatc tgtgaattca cttcgctctg
    14461 catcgcattc agctgggcta aagcagcatc aagctgggtt tggtcctgac tggcagcggc
    14521 ttcggccgag gcaggggctg tttttttaat cataaaccaa acggccaatg ccccgactgc
    14581 aaagactgct atcagcagta ttgtgctctg ccgcactttt ctgccgtgac cggaaactgt
    14641 cagatagtcc gcaggccggc tggctgtatc cgggctttgc gccgcctgct gggcctgctc
    14701 cagaaccggc gactgacttc cgtctttgag gtaatttaac ataacgattc tccttgactg
    14761 tacagtaaag ggttcaggcc gcactctgga agaaaatgct catcacaaat gtcgcttctg
    14821 tctgaccttc ctgtttgctt ttcttaagcg tcagctcgcg aatcttggta atgcggtcca
    14881 gtttctcaag ttccagaaga aacgcatagt acgagttgaa attgccgtgc agttccatcg
    14941 acagcggctg ctcgatatag ccgcggttct ggcggttttt gagggtgcga atcgccttcg
    15001 gggttaatcc ctgccgctgg gcaatcaggg ttacattttc cagcacagta tgaatttcgc
    15061 tgctgggagg cagcttgctt tcaaaagcgg caatggcttc ttcgattttc tgaagctgaa
    15121 cattgaggtc ctgtgccgtt gccgtcgccc gctctagttc gttgagcttt gcctgcaggg
    15181 cttccatctt ggcccgttct tgtgtcagat gggcattggc cggcttaatc atatacgccc
    15241 aagccacata cgagagtccg acaatcagca caaaaaagat taaatagcga atcccgctgt
    15301 tcattacaag gttcctttcg aataaatcgc ttatccgctt acatcagctg attccgtttt
    15361 tcacggatcg agtcaatatc ctctttggtc agggtcaggc cgggtttaag ggcggctttg
    15421 agccgaaatt cgcgcatcct gattttgtct atttccactt ccttggattc aaccagcgaa
    15481 acagcatcaa ataaaatcga gctgctcagc cgcgcaatga agccggccac ctgaatatcg
    15541 ctgggcgcca gacctttaat gtcgaggttc gtttcgatac gaacttgagg ctggtcggtc
    15601 ggctcggatt tggctgtggc agccgcccgc tgatactgat tgctgccggc cggtttggct
    15661 ggtgatggac gggctgtctg aacttctttt tcctccagct tgaattccgt cagggagacg
    15721 cccgaaggca ggttgttggt cagagacgcc aatattacac tgcggggaac aggttccagc
    15781 agttcggcgg tcatcaccat tgttttcatc atggttttgc ttttggcctt cagctcttcc
    15841 agctgggcaa tctgctgctg ggccttggcc attcgttcat tgagctgagc cagctcggcc
    15901 tgcacagccc gctgacgcat cttgatgaag ccgaatgtaa tgccgatagc accaagcata
    15961 gccatcagca gcatcaaata cagaatattt gcccggctgg cacgccgctg ctgtacataa
    16021 tcatctggaa caaaattaat agttgccata atcgtattcc tatctgatta acttaatcct
    16081 tctaagctca gcccgaaggc tgttgcccag tctacccggc tgttccgccg gtcaataaga
    16141 cactggggcg tttggtttat ctcaatggca ctggtaacat cacctacctg tgcaggaatc
    16201 tgcatccggc gagccaattc agcaaaccgg tcgcaaagcg tgcggctggc gccggatccg
    16261 gccagaaaga ccagccgctc tatccgagca cccgaaaaac tttcctcaaa ataccgaaca
    16321 caggcatcca gttctgacag caaacgctgc agcatctggg tctgctcaag ctgggcatat
    16381 ccgattgaaa tagcacgggc aaacagcaga tttgccccgc gggttatgac cacattgcta
    16441 tgggaagtgc cgacggtcag caacagcgcg ataacattct gctcgttgct gcggcggcag
    16501 aaaaacttgg aaaagcttgt aatcatggcc gtcggccaga taccgatttt gctgacttcc
    16561 agtccggcgg cttcgtaaat cgcaagatga cgattcactg cttcacggtt aactgctata
    16621 gtcaagatat ctgcgctgtc cgaaggggct ctctcggtcg gctcggccac tatatactga
    16681 aacagagcat cattaaccgg aaacggcaga cgcttctgga gtttaggcgg tattacttca
    16741 tcgagccgat ccacggtcgt acgcggcacc ttcaccggat cgataaacaa atcatccgtc
    16801 ggcaatgctg tgataacctc tttgcctttg aactgccctt ctcgcatgag ggagcgaaca
    16861 gtatctactg cccagtgctg ccaggcggga ctgcgaaact caatgtcggc agggacagca
    16921 cgcaaagtgg cactgtgcag atagagtccg tttccattcc tgccaatctg caccatccgc
    16981 aaatagccac tgcccatatc gacgccgatg gggcaggttt ttttgctgtt aaatcctaat
    17041 accatacata tccttaaact tacaaatcgg gctggtttgg aattatctgc ccaattgtct
    17101 cagaaaaata caccttaaac agtttggttt cttctggtgt gtcgtccata ttggcggcag
    17161 ggttaaccag ccaaattctg acaatcgctg ggatttgacg gcaaataccc aataaaatct
    17221 aagaaacaac cccggagcgg tcaaaaagat aggttgaatt cttaatcaaa aatctacgcc
    17281 cgataatatt attgctgata agacaggact acaaatattg aaaaaagccg attaattttg
    17341 agctttcaaa aaaaggataa tctggacaat tttgtgctga taaaatctat tcagatatac
    17401 accaagatac ttcgaagtgt ccgataaata aaattgatgt acccgccttt attagagcat
    17461 tctgctccct caggcacctg ccgcaggccc gttattctat gtttcgagtg ttgaatgttt
    17521 cgagataact tattcgcggg ccagttcgat atggtatgaa aagtgcttgt catcacggtt
    17581 gggataatcc tgtcggtagt gaactccgcg ggattcctga cgcttatagg cagcatgggc
    17641 catcagcaac gccactgtca gcatattctg acattcccaa ccaaacggct cgctgaaaac
    17701 cttgtccatt acataacgct gccagaactg gataatttcc atcgcctctt gcagcggtgt
    17761 tgcactccgg gtgatgccga cattgcgcca catcaaagcc cgcaacgaat tccggacatc
    17821 atcggtatcc aatcggctcc ggtcggacgg cggtacatca aactgcatcc ggcgataacc
    17881 tactgtgccg gcttttgcca gcagttccac agcagattga ccagcaatgc gtccaaagac
    17941 cagcccttcg agcagggaat tgctcccgag gcggttggcg ccgtgtaggc cggtcgaggc
    18001 agcttcgccg caggcaaaca gccggggaat gctggtggct gccgaaatgt cagctttcag
    18061 gccgccaatc atataatggg cactggggcg aacaggaatc aggtcggttg atacatcgat
    18121 atcgaaactt tcacacagtt cactgataag cggaaaacgc tttttgaaat gggctttatc
    18181 gaagtggcga atatccagat aaacatgtgt tgcctgtgtt ttaagcatcc ggtcaagaat
    18241 agcccggctg acaacgtcac gcggcgccag ctctcctctg ggatcatatt ccttcataaa
    18301 tggttcatgg ttaatgtcgc gcagaatagc cccctcgccg cggagcgttt cggtgaccaa
    18361 ggcccgtgag gcaccggcaa tatagagggt cgtcggatga aactgcataa actccatatc
    18421 ccgcaaaaca gcccctgccc gccaagccat tgccagaccg tcagcggtag cacattccgg
    18481 attggtggtt tcacggtaaa gccgacctgc gccgccggta gccagaatcg tggcaccggc
    18541 ccagataatc tgcagggacc cgctggggcg gcgtccgatg acaccgacgc actggccatc
    18601 ttcgctggtc agcagatcta ttgtgaaaaa atgctccata acattaattg tcgggctttg
    18661 ctggacacgt gcgataaggg tttcggcaat gccgcggcct gtagaatcgc catgggaatg
    18721 agcaatacgg gcatggctgt ggcccccttc cagcgagata tcaatttcgc cgtccaccaa
    18781 atcaaaggct gttccccagt cagccagctg gcgtaccaag cccggacctt cccggacaac
    18841 ccgttctata acaggtttgt cccccagccc gcatcctgtg cggagggtat cttcaatgtg
    18901 agcctcaaaa ctgtcctcaa cctcgtccag caccgatgca atgccgcctt gggcattcca
    18961 cgtgttggtg tcagagaagg tgcctttgca gaccagcgtt acattgcagt gcggggctgc
    19021 ttccagcgca gcgcgcagtc ccgcaatgcc ggctccaata actaaacagt cggtaaacaa
    19081 ctgctgtgtg gaagcggtgt ttatcggtac caaataccgt cgaatatcgg cttgtcgtgt
    19141 cggcattctt gcagtcctat gcccggctta atgcgttcca gtcgcataaa ctcaaagtat
    19201 caataatcag cgggctcagc ttgccgtttt cgacgctgga gtcctcatca aacggcacca
    19261 ctgccagcag atttaccctg ctgatactgc agataacatc cggggctgtt tcctcagcag
    19321 tatcggctcg ggcggcatta tacccgctga taaccaatcc tgcaacaggc aggccggcat
    19381 ttcgggctgc ctgaatcgtc aataagctgt gattgatggt acccagattt ggccgggcag
    19441 caataacagc cggaaggtca aactctacag ctaaatccag aactgtttca gtcggggtca
    19501 tcggcaccag aattcccccg atgccttcaa ccagtacaat atcacaggta tcacacagat
    19561 acttataagc cgctgcaata gcatcaaaat caattggtct gccttctatt tcagcacaga
    19621 ccagcggtgc agcaggcgtt ttgtacgtca ccggcgtaat gacgctcaag gggtaatccg
    19681 catcggcaca tagagccaga aactcggcat cagtgctgac cagcccctcg cggctaggcc
    19741 ggcagccggt ggcaatgggc ttgaaaacgc cgactttaag gccctgccgg cgcagaacag
    19801 ctgctatggc acccgttatc aaggtcttgc ccacgccggt atctgtgccg gtgataaata
    19861 aaccattctt tttgggcagt cttaactcaa gcatactcat tccaatttat gattgaatat
    19921 caaattgcac tatgtcgact cagttagata cagatttcct tttctcatcc ggcagccata
    19981 cctccattct ggcggtattt cctctgttat cctgacaata aaacggaatt gctgtcaatg
    20041 taacgttttg agcttgtccg tttggcccaa tactgaatgc ctccgctttg atacaattca
    20101 ctccgcccaa aaaatctgga ttgtattcca aaaccaatgg gctgcctgtt ttcagagaaa
    20161 tggtgtctat tgccggatta tcctgttgtt cgatacagta aacaagcggg ccagattgga
    20221 tcgccagccg ccccctgcag gccttcacct ccggatgggc atagatacga cggggctgca
    20281 ccggaagctc cagaatgact tcatctcctt tgttccatgc ccgtttgata acggcatagc
    20341 cccgacttat ttccgatgca gatatacttt ggccgtttac gcgtaattca aactgtgagg
    20401 ccaaatccga ggtgtagagt ccaaacggat tttcctgtcc ccttgcccat cccggtatgc
    20461 gtacaaataa tgcaaattct gtctgtgctt gggtatccag cacaattttc acgttacctt
    20521 tccaagggta ttcggtttgt tgcacaattt tcacagattg attttccagc ggaattacag
    20581 ctttgctgcc gataaacaga ttcacataga tttcctgact gtcataggca taaatataac
    20641 ctcccagccc cgagacgaat tttaaaaaca tcgggggaca acacgggcag tcatgccact
    20701 gccagcgcct gtgtccgttg gccgatagcg gattttgata ataataacgg gtaccgtcga
    20761 gagaaacacc attaatcaca ttgttgtaca aggtgcgttc cagctggtcg atatatcggg
    20821 catccgcaaa cagcagattc atattgaaat gaaaaaaacc agccccgact gctgcacagg
    20881 tttccagata cgcatcattg ggaagggtat aatccgggcc gaatttctct tcatgtgcga
    20941 acgcccccac gccgccggtg atgtgcatcc gctttccgac catattttcc cataggcgaa
    21001 ccgccgcatc cgcataggcg gttttgccat taacagccgc ggcggcactt aaacccgtgc
    21061 ataacagcgt ggcacggaca gcatgccctt ccattgtttc ctgctggaaa acaggtttgt
    21121 ggtcttgtgc gtactctccc cagctgggtc tgccgctgcc atacttctgc tggcgtatcc
    21181 attcttcgca tccacggccg ttacgatccc acatttcaga ggccggctgg ccgcaatgac
    21241 ggccgcgcat ttcaatccag aattcagcaa gatgaagata agccgcttca tccacaggaa
    21301 cattcagcct ctctttcaga gaagggcttt cctgaaacag cacatacagc ttcatcatcg
    21361 cctcttctgc cagtgaatgc gcaggaacga tatttttttt cggctccggc ccgatgattt
    21421 gggtcatata gttggccagc tttacagcag ctctaagcaa atcgatcttg cctgttgcac
    21481 gataataatg aattccggct tctaccagcg cgccggcatt gtaaacatca tgctgccacc
    21541 gtaaaaaacc gccattcaat ccccagcgat gatcgggctc ctccagctgg gtgtatgtca
    21601 taacatagcc gtcgggatct gccgcctgtg cagcagcaat ccgctgaata tatccatcaa
    21661 ttcgggcttc aagttcttta tccggatagg caaccaaaaa atcggatgct ccgcgaatgg
    21721 tttcatagac aagcacgtca aaccaaggcg gtccttcatg ttttggctct aacttgccgg
    21781 caatccggtc gaaattacga aaagccccag ctgcttcaaa tttatccagc acattcttta
    21841 atgttatcga attccagatg ttgagcttcg ggctccagaa cgcatcttct atccggacag
    21901 atcccgcagg gacaggtttg agaagatgaa tttcctctgc agaaagcaga cctacggcaa
    21961 cgagaaaact tatcacatac gccaattgct tatacagcat tttttctctc acactaagtt
    22021 aaaaagaggg gaatcaggcg tttttcagct tggttctgat attttttctg gacaattgac
    22081 ctctgttgtg ttatattttt agattagtaa aaatcagaac aatgtcaaac gaaaagtttg
    22141 tctattccgc tcaaataaac agacgatatg aagcagtatg taactatcat cacattgggc
    22201 atcgtcttat gggcagcccc gctattggaa gcaatggata atccgttcag ttatgaagac
    22261 ggcatcaacg actgcgatgt catcaaatat aacggcgaat attacatcac aggcaactgg
    22321 ctgggcggcg atatgttccg ctcgcgcaat ctgaccgact ggggggaacg aatccatatc
    22381 ttctcgtgga acaatacttg gcacgtccag agaaatgcca atcgtgatat ggatattcac
    22441 ggcacccata ttgcctatga caatggacag ttccatttat atgcccatct ggatacacct
    22501 gccggcaatc tgctcggaat tgtccatgcc gtcagcaatc aagtgacggg accttatacg
    22561 gaacctgttg atgcaccgtt tgcgactgat actattgatg tcaaaacatt ccgcgatgaa
    22621 gatggttccc ttcattttta cagcacccga ttcggcggcg tcagcgccaa ccacaacgat
    22681 taccgcagca tgcaggatta cagcacgttt accagcggct ataagacatt aatctggcct
    22741 accggaggct gggaaattca tcctgaactg ccggaattca gctatggtcc gaccattaat
    22801 gaaggacctt tcgtatttaa gtaccgcggc aaatactaca tgctgtataa tgccaaccat
    22861 accggcgata catcctatgc catcggctgc gcaatggctg acacagccag cagtttcagc
    22921 aatgccggca aacttccgag tccggttctc ggaaaaacg
//
LOCUS       DAOTBO010000036        22910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1850_length_22910_cov_5.903347, whole genome shotgun sequence.
ACCESSION   DAOTBO010000036 DAOTBO010000000
VERSION     DAOTBO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 22910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22910
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_22910_cov_5.903347"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /gene="folP"
                     /locus_tag="PLE88_05990"
     CDS             <1..480
                     /gene="folP"
                     /locus_tag="PLE88_05990"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOM76049.1"
                     /translation="TALADERMTALALKHKVPVILMHMQGTPETMQAAPFYEDAASEV
                     FAFLKERAAAAQEAGIPAEHIFLDPGIGFGKTTEHNLQILSRLDRLCRLGYRVLVGTS
                     RKRFIGQITGKDRPEQRLFGTAATVALAVQKGVSIVRVHDVEPMVDVVKTAAAVLRA"
     gene            complement(529..960)
                     /locus_tag="PLE88_05995"
     CDS             complement(529..960)
                     /locus_tag="PLE88_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76050.1"
                     /translation="MKKTSIIAVLAAVLWLAGCENKELTTCQQDNQTLQEQVHTLQQQ
                     LQQAQAAAAEKDQKIEALKAENTKVQTQAMESIKTMMEKQAAHDAEVKKNLDAKTQEA
                     KDLQQKLQASQAVMEDLQKQLKAAQEQLKAAAPKPAEAPAQ"
     gene            complement(997..4794)
                     /locus_tag="PLE88_06000"
     CDS             complement(997..4794)
                     /locus_tag="PLE88_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM76051.1"
                     /translation="MKQIVLSTAAAIGILCMASAFSIAQTVPDWENPAVFGINKEKPH
                     CTLIPYESAGKALQADRSRSDWYQSLNGKWKFRWSPDPDSRPVDFYKPLYNVQSWDTI
                     PVPSNWQLLGYDTPIYSNTRYPFAKNPPRVMDTPPVQYTTFQMRNPVGSYRTEFSIPS
                     KWKDRQVFIVFDGVESAFYLWINGQKVGYSQDSRTPAEFNITKYLQPGRNILAAEVYR
                     FSDGSYLEDQDFWRLSGIFRNVYLYATPQLHIRDFFVRTDLDDNYRDAVLDVETELVN
                     FASSPAAAGLTVELVGANGSKTASLTVDKIAVAAGSTAVCRPSLKISNPAKWSAEAPN
                     LYTLVLTLQDSSGRTLEYLSSRIGFRKSEIKGGQLLVNGQPIYIKGVNRHEHDPDTGH
                     YVSRQSMIQDIMLMKQNNVNTVRTCHYPDAPEWYDLCDEYGLYIIDEANIESHGMGYG
                     AESLAKKPEWKEAHMARTQAMVERDKNHPCVIIWSLGNEAGDGPNFEATSAWIKQRDP
                     SRPVHYEQAGQKPHTDIVCPMYATIDTIVRYASREQTRPLILCEYEHAMGNSLGNIHD
                     YWVAIEQYKHLQGGSIWDWVDQGLRKKPDTVFTVKDLSPSKLTAVLNGKLTEGPGGAK
                     AVSGYLEVEPADALDITGTALTLEARVKPFRAGQEQHQPIIAKGDQQYSLKINGERAE
                     FFIYDNTWHSVYADLPDNWFGQWHHLAASYDGSALKLYIDGKLAGTETYTGRIAHCDY
                     PVGIGIDTQNRGRRFGGEIAKVRIYKAVLSDAQIAAANAKPDASAVLQLDFNPSDIKK
                     TTPSKDVWFWAYGGDFGDYPNDGNFCCNGLVQPDRKPNPHLYEMKKVYQNISVSADDL
                     SAGKITIRNKYFFIPTDFVDVQWELAESGTVIQKGTLGAVSIQPQQSQSVTIPFAKPA
                     IKPGREYLLTVRFVLNADQPWAPKGHLMAWDQFVLPFEPQPAPAISLETMPTLTVEEN
                     TGSILVKGQDFVLTFDKAAGALAGWNYRKTELIASPLVPNFWRAPTDNDNGNNMPNRC
                     GIWKNAGPKRTVSDVAVKQLCPQAVRITVNCRPAAGDTALQTVYTVYGSGDVLMDNTL
                     TCGANLPELPRIGMQMEMPGQFSTMTWYGRGPQESYWDRNTGYAVGLYRQDIYKPDHI
                     YVRPQENGNKTDVRWAAWTSKKGIGLLAVGQPLIYASAWPYTMADLEKARHRHEPAFR
                     DTITVNIDYKQTGVAGDNSWGARPHRQYTLWPQTYQWQVRLTPVKNADAVKQAVFQTL
                     PKL"
     gene            complement(4899..5813)
                     /locus_tag="PLE88_06005"
     CDS             complement(4899..5813)
                     /locus_tag="PLE88_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76052.1"
                     /translation="MTISCTCPQCGRVCGFKDQYAGRRARCLACNARFIIPAADGAKA
                     VPVQPSPQEPLPGFFRAALADNFSVFIQKESLFGIILSAALIAFHFFAGNADYSFTVG
                     HFRPPLIVGWIVTFGCAGYLLWYCMEIINSTFMDNDFLPDIFVGTGFAFLGESVKNIY
                     LFIAAAAAAAIPGSAITALLEIIGLTHQGLQTAIVLLSLTMWPMMLAMLGSGAAPWEL
                     FRYDRIIRMIAKTAGAYLLTASVTFAALLAVYVTVGLFAQDIKSSAGILAALAARLAA
                     AGALLFAMRTIGLYARHYFRCFPELFPS"
     gene            complement(5815..6951)
                     /locus_tag="PLE88_06010"
     CDS             complement(5815..6951)
                     /locus_tag="PLE88_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOM76053.1"
                     /translation="MEQTYTIDDKCYPAGEGNFLSINDMLAYFEKMGVMRVSDLHIKN
                     GTPPAYRIDGELVRLKGAAVSQTIAERLIYPLLGVKNIESLKRDLAVDCSYKFGSLQF
                     RINAFMDNDGLAAAIRALGTHIPAPETIGFPNNAWQDIVRRKHGLILFTGITGAGKST
                     TIASLLDRINENRACRIITLEDPIEYIFTQKNSMISQRELGRDVDTFAHGLRSMMRED
                     PDIIFVGEMRDSETVALTLTAAETGHLVFSTLHTRDAVGSITRILDFFPHQKQDEVRN
                     QLSLGLRYIISQKLIPRKDGTGRVVAMEILNSNYALANLIRKGKIEQMYSQMQVKTKD
                     QPDERMITMERHLALLVKKGIVDILEAQKWANDLKSFQDAMKND"
     gene            complement(7052..8266)
                     /locus_tag="PLE88_06015"
     CDS             complement(7052..8266)
                     /locus_tag="PLE88_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM76054.1"
                     /translation="MDINYRQIIRRTLFGLLIAAAAIGAMIAFILPYKRIPPAPPLPP
                     AVRQPASFGNQAWPIFRGDAALTGQAAGTLPDKLKPDWTFKTKNAVKSTPIVADNKTF
                     ISSMDSCLYALELHTGREIWRFEADEALEASPVYHNGMVYVGSNRGTFYAVKAQTGQL
                     VWSCKTDGKITGSANIAVHPKTDQTLVIFGSYDNNLYAVDAAGGQIVFQYPARNYING
                     AIAAAQNTAAFGSCDANLYLVPIDAPEQTRTIDAGSYVAANPVLFEGVIYAGNYDGVF
                     LAADAAGGSILWRFDKAEDAFFSSPAVNDRVVVVGCRDGKVYCFSRQSGKLLWTFAAQ
                     DSFDSSPLLCGDKAAVGCSDGRLYLLDINSGKELFAYTLGGSVSSSPAAAQNRLLVGC
                     EDGSVYTFVQAD"
     gene            8419..9483
                     /locus_tag="PLE88_06020"
     CDS             8419..9483
                     /locus_tag="PLE88_06020"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOM76055.1"
                     /translation="MAVGKAFFGTTSDGQKVDCFTLNNARGRIAKIITYGGIVTELWM
                     PDRKGVLDDVVLGFDNLADYETKSPYFGCITGRYANRIAEGRFTLDGVEYSGLAINNG
                     ANHLHGGIKGFDKVVWQAEPFENAEGKGVKLHYLSRDMEEGYPGNLDVTVTYTLTDAD
                     ELKIEYAAVTDKPTVCNLTNHSYFNLAGHGYGTHYEHQLMINADAFTPVWDAGAIPTG
                     EILPVKGTPMDFTRPTPIGLRIDENYDQLVYGSGYDHNYVLNKSDAALSLAARVSEPT
                     TGRVMECWTTEPGVQFYSGNYLDASLVGKGGKGYGRRFGFCLETQHYPDSPNKPNFPS
                     TVLRPGQKFRSTTIFKFLTK"
     gene            complement(9619..10710)
                     /locus_tag="PLE88_06025"
     CDS             complement(9619..10710)
                     /locus_tag="PLE88_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOM76056.1"
                     /translation="MTDWKNPPKLFIPGPVHVVPDVLQQLARPTLGHRRKEYSQLHSE
                     VTQMLKKVLFTEQSVFLSTSSASGIWEGSIRNCVRKDETILCTMCGAFSEKFGDVARS
                     CGRQVEELRVEWGQAITPEMIDKKMATGKYPVLTLVHNETSTGLTNPVYEISAMMKEK
                     YPDVLVLVDSVSGMVGMPLEFDKLGWDVVFASVQKAFSIPPGLAVFAVSNRALEKSKT
                     VPERGYYFDFQEFAKSAAKDQTPTTPSIPHIMALHYQCTKLLAEGMENVWKRHKEMAD
                     YVRNWGQEHGFELLPPKQYASNTLTTFKNTRKIDIAAVNKALQQKHNVVFGNGYGKLK
                     DLTFRIAHMGDTTLADLKELLGWMAEEIK"
     gene            complement(10707..12074)
                     /locus_tag="PLE88_06030"
     CDS             complement(10707..12074)
                     /locus_tag="PLE88_06030"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOM76057.1"
                     /translation="MHIKPFRAYRFNPEVVGNVGDCIAPPYDVIDAAMQEQLHQRNPW
                     NIVRATRGKTSPDDTPQSNVYTRAAEFLEKAFAEKALIAEPYDAVYAYVQDFQIGQRR
                     LERSGIIALGKLQRYGGGVQPHEKTLEGPKADRLNLTRATACQLGQIFMLYDDPANTD
                     RTVIDSAMKQTPVVDTIDTEGVRHRLYVISDVQLISMFAQMMTNKQTIIADGHHRYET
                     ALNYWAETQNEQASWQMMTFVNMRNEGLVIQPTHRLLVNMPDFDSTALLDGLGSEFDI
                     AEYPFQNKAQKQQAQKAMFGKMEAQRNSNCFGFYAGSGAFYTIKLKNTETMSRLYPDM
                     SEAAQKLDVNVLHKLILEKHLGICDVKLASQSHLVYIKDLGDAIEKSIARVDSGRSQG
                     VFFMNPTRIEQVQAVASAGEKMPQKSTFFYPKIFSGLTIRKMDIQPIQPQFTQSNLIE
                     EECSK"
     gene            12398..13255
                     /locus_tag="PLE88_06035"
     CDS             12398..13255
                     /locus_tag="PLE88_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM76058.1"
                     /translation="MKKAFTLIELLVVIAIIALLLSIVVPGLRKAKDKTKDVICRAHI
                     KGIGVAVLLYLSDNESRAFNNRESNGHLWYDAAGKIITPDNTVWWADAYWGLGYRMYA
                     SDPRVFSCPSFVLKDVAGLMYSDHPNYKTTRTDLTRASGYGLNSFFFRDPEAPASDVY
                     RNHRKISTIRSPSRFIVAHDHMEPKMEGNSSGSEQGDTLYVPSGSTFNLVQYRTGTRQ
                     PFYGGIFRHSKKSKNWDEPSQLAARLAQIDKNPNGNVNVLFADGSVDKISETTGQNIP
                     YTMYSGLLK"
     gene            complement(13280..14593)
                     /locus_tag="PLE88_06040"
     CDS             complement(13280..14593)
                     /locus_tag="PLE88_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM76059.1"
                     /translation="MNTTNILFFLFIGLLMLIPSAAEGNSQTPGLSIQSTHSTAVIER
                     RGERFELLLNGKPYFIKGAGGARYLEKLVEAGGNSIRTWSSSRQALDSAGRYGLTVCM
                     GLSMQSPRHGADYRNEKWLQEQRERICKEVEELKSHPALLLWAVGNEVEHLAQPEDCI
                     LVWKEIEILAKNIKAIDPHHPVITVIAGAGKKLEDIKQLCPTLDAIGINSYGTLSFIP
                     SEIRRWGWEKPYIITEFGPRGWWEAPRTAWGLPIEETSTEKARFYFEAYKAAIEDKPN
                     CLGSYVFLWGNKQEKTHTWFNMFLPDGTPTEMMDTMTYLWTGKWPANKCPSIGDKKIW
                     VEGNEMHHIYRPSQEVVFRVQANDPEGDQMTAEWDIRKDESDNPSVGGDWERRVPPID
                     GAVVSSEADRAVIRMPQESGNYRVFVYIRDASGKTATANLPIQVK"
     gene            14969..16423
                     /locus_tag="PLE88_06045"
     CDS             14969..16423
                     /locus_tag="PLE88_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOM76060.1"
                     /translation="MKLIIIVCLSVSVSVLGQNLLINGDFESGTGGWTKWWNSSNAGF
                     PVSDPVEGDNCAGVWWSDVGIYQRLSIGPGLYTVSGQMLHTSAAPLGNNRIGIIQAEV
                     GNGTQIWWKQQIVIDQTSPRDKWIDGQLIVDNRTAGASSLNINLFMYDRDGEHTGNGI
                     VRYDNLSVTPEAAVNDPDYNDDKAVNLLDFSEFSGQWALESLSCNLSGPAVIDMDDLA
                     VFAEAWLMDTPTYPGYELVWSDEFSGSSLNLADWEYMIGDGCSYGVCGWGNNELEYYR
                     AENVSVQNGMLIIEARRENYGGKQFTSGRIRTANKRDFLYGRMEARIKVPTGGGMWPA
                     FWMMPTDSVYGGWAASGEIDIMETRNSTNFIQGTIFYGGGWPNQQSSTGKYEPAGVDF
                     SQDFHVYTLEWEPDIMRWYVDGILYSTKTSSQWYSDGAPGNNRAPFDQRFHLLLNTAV
                     GGNYTGCTSASCITAVFPQQMLVDWVRVYQKTAP"
     gene            complement(16455..16646)
                     /locus_tag="PLE88_06050"
     CDS             complement(16455..16646)
                     /locus_tag="PLE88_06050"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOM76061.1"
                     /translation="MTAETNFEQMAREKRPTLLREFWEFLKHNKKWWLLPIILMLLAI
                     GLLLLLSSTAIAPFIYPLF"
     gene            complement(16656..17075)
                     /locus_tag="PLE88_06055"
     CDS             complement(16656..17075)
                     /locus_tag="PLE88_06055"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOM76062.1"
                     /translation="MTLIKPPLNPSDKELRTFGFACLTAAAAASLILFKTARATGVQC
                     LFIFFLGAAVCGLGLIRPLWAKPVYRVMILLGFPIGWLLSHMILAFFFYCIFTPVGLC
                     RRLLGNHPLRRYDESRLETYWIPRPKKPTAKDYFRQF"
     gene            complement(17081..18925)
                     /locus_tag="PLE88_06060"
     CDS             complement(17081..18925)
                     /locus_tag="PLE88_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOM76063.1"
                     /translation="MTKPVYILGISAFYHDSAAALLKDGRILAAAQEERFSRKKHDAD
                     MPTNAIGYCLSEAHIGPEDLDYVVFYEKPLIKFDRLLETWLDYAPRGFGQFLTAIPQW
                     LHQKLQIPRHMDKVLGGGFKKSFIFTEHHEAHAASAFFPSPFQEAAVVTLDGVGEWTT
                     ASIGIGKDSRIDLLYELHFPHSLGLLYSAFTFFCGFKVNSGEYKLMGLAPYGRPVYAD
                     LILNNLLDLKEDGSFRLNMDFFTYGYTNYMIGKKFAQLFGGPARKPESPLTQREMDIA
                     ASIQKVTEEVMLRIVRFAGCKTGMKNLVMAGGVALNCVGNGRILREGPFDNLWIQPAA
                     GDAGGALGAALFVHYQLLNNPRIADGQQDQQEGSLLGPAYSNEYVRSRLNAAGAKYRF
                     FDQEEDLLARVAEAIEAGQVVGWFQGRMEFGPRALGSRSILGDARNEHMQSRMNLKVK
                     FRESFRPFAPSILQEDAAEYFELCQESPYMLLTAAVRPEHRIPLTPQQESCCGLDKLK
                     VKRSMLPAVTHVDYSARIQTVNPQRHGRFYRLLKQFRQKTGCPAVINTSFNVRGEPIV
                     CTPDDALKCFIHTDMDVLAIENFILYKKEQPQTFLSAAGNYPVLVNPD"
     gene            complement(18918..21125)
                     /locus_tag="PLE88_06065"
     CDS             complement(18918..21125)
                     /locus_tag="PLE88_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM76064.1"
                     /translation="MKKQTPAKQNLSIASSAGSSSRARLWLFRLICVFVGPILFAGIW
                     ECGLRLLGWGYSTKAIVKCTLNGEEFCCHNYQFGWQFFPRRMARNFDGFVFKAEKPHK
                     TCRIFVLGESAAMGMPAPAYNFGRILQVMLEELYPGVRFEVHTAAMVAINSHAVLKIA
                     EECTRYNPDVLILYMGNNEVVGPYGPGTVFSPLLSSLGLIRANLAVKSTRTGQLLEQA
                     LDSAKFRNPAPSRWGGLGMFLGKQVRYDSPAMDYVYRHFEQNLTDICSIAGRRRIPVI
                     ISTVGANLKDCPPFASLHREDLTKAQLQEWETLYQEGIDYETAGQHQSAVEKYHAAEQ
                     IDPTFADLQFRLGRCYWLAGNFTAAKNHYRSAVEYDTLRFRADARINQIIRSAAEKFK
                     QSGIVFVDGAAALEEHSPQQTPGGELFYEHVHLNFTGNYVLATALLPAVQQCLSGFPA
                     SSAGAVLSEQQAAERLAYTPFERHDFLNRLYQKMYSQPPFTNQLYHAEFMQTVQQQIR
                     QLETMLQKAGISDCRRQYTQAMQRRPDDWQILWQYAVFAGTGLNDIKAQEEALRKVIR
                     LCPYDAAYLSLGKNLHQQGKLKEAQEILRRLLDLKAGYDGKAHLELAMIYRQIADTSN
                     YIKHLSKAIWFDPAGSIEAYGALAEAYEKSGELNKAVQTLYRAVAIFPRQQTAAAHAT
                     LGYMLNSQGNYEKALEEMKIALEINPAFAEDKLFKSLLSHIETRLSRRQKTND"
     gene            complement(21210..>22910)
                     /locus_tag="PLE88_06070"
     CDS             complement(21210..>22910)
                     /locus_tag="PLE88_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76065.1"
                     /translation="RAELERYRRDYGVYLPVQLGLYIVGIENEIAAGLWDADIERFCR
                     DLNQLGYPLYIRIGYECNGDHNHYDPIAYKAAFIRITNALRSNNVEAATVFNVIQGPY
                     QSWYPGDAYVDWMSINAFGVWNIQHPYTPLFLNDAHARGKPVLIGEASPVFWHTDQGE
                     ASWNGDFVPYFNLINSWAGIKNFCYINTNWGGQWGDCRLQPYPIVAQHYKDQMDSALY
                     LHGTDEVSLRADITGIIDTIPPAKINDITVTATQHGILLQWQPPADDVGINRYEILRD
                     GILAGYNTQHYFEDTTVAAGKTCRYQITAIDKGGNRGPVSEPIMAVAAPSIDKVINGQ
                     FDQGRGPWATAWNATGLSMSSSIDSTSKLSGENSCKLIINSVSGIPWHLQYYQNLNTQ
                     AGFRYTLTFQAAADRNTSISVALQETHSPYSTFISYTADLTPVPQTFTVSGISPDKDN
                     VNLAFMLGASAPRTIWLDAITLTETSSNPDPQNCSEVYAAGFELPADLSGDCSVDIED
                     LIVWAAFWLDANCGPDNGYCQKADAGLDGSVTLADWEAFCLYWLECNDPHNSSCQPTW
                     "
BASE COUNT         5342 a         5672 c         6169 g         5727 t
ORIGIN      
        1 acagcccttg ccgatgagcg gatgactgca ttggccctaa agcacaaggt gcctgttatc
       61 ttaatgcata tgcaggggac gccggagacg atgcaggcgg cgccgtttta tgaggatgcg
      121 gcgtcagagg tttttgcatt tctaaaagag cgtgcagcgg cggcacaaga ggccggcatt
      181 ccggcggagc atatttttct tgatccgggc atcggcttcg gcaaaaccac agagcataac
      241 ctgcagattc tcagccggct ggaccggctg tgccggctgg gctatcgtgt tttggtcggt
      301 accagccgca agcggtttat cggacagatt accggcaaag acagacctga acagcggctt
      361 tttggaacgg cggcaacggt ggcgctggcg gtacagaaag gcgtttccat tgtgcgtgtg
      421 catgatgtag agccgatggt cgatgtggta aagactgcag cggcagtttt gcgcgcataa
      481 aaaagccgtc tgcttcacgg gcagacggct ctggaatggt aatcgctgtt attgggcggg
      541 tgcttcggcc ggtttcggag cagcggcctt gagctgttcc tgagcggctt tgagctgctt
      601 ttggaggtct tccatgacgg cctgcgaggc ctgcagtttc tgctgcaggt ctttggcttc
      661 ctgtgtcttg gcgtcgaggt ttttcttcac ttcggcgtca tgggcggcct gtttttccat
      721 cattgtcttg atgctttcca tcgcctgtgt ctgcactttg gtgttttcgg ccttcagggc
      781 ttcaattttc tggtcttttt cggcagcagc ggcctgtgcc tgctgaagct gctgctggag
      841 cgtatggacc tgctcctgaa gggtttgatt atcctgctga caggttgtca gttctttgtt
      901 ttcgcagccg gcaagccaca gtacggccgc cagcacggca atgatgcttg tttttttcat
      961 tgtcagtccc tttctaaaat gaaaatcctt tttaatttac agcttcggca gcgtctgaaa
     1021 aactgcctgc ttgacagcgt cagcattttt tacaggggtc aggcgaacct gccactggta
     1081 agtctgcggc cagagtgtat attgcctgtg cggacgggca ccccagctgt tgtcgccggc
     1141 tacaccggtc tgcttgtagt caatgttaac cgtgatggta tcgcggaaag ccggctcatg
     1201 acgatgacgg gccttttcca aatcggccat ggtatagggc caagcgctgg catagataag
     1261 cggctggccg acagccagca ggccgatgcc ttttttgctg gtccaggcag cccatcgcac
     1321 atcggtttta ttgccgtttt cctgcggccg cacatagatg tggtcgggct tgtaaatatc
     1381 ctgccggtac agaccgacgg catagccggt attgcggtcc cagtacgatt cctgcggacc
     1441 ccgcccatac caagtcattg ttgaaaattg ccccggcatt tccatctgca tgccgatgcg
     1501 cggcagttcg ggcagattag cgccgcaggt cagggtgtta tccatcagga catcgccgct
     1561 gccgtagacg gtataaacag tctgcagcgc cgtatcgccg gcggccggcc tgcaattgac
     1621 agtaatccga actgcctgag ggcaaagctg cttgaccgcc acatccgata cggtccgctt
     1681 gggaccggcg tttttccaga tgccgcagcg attgggcata ttattgccgt tgtcgttgtc
     1741 ggtgggagcc cgccagaaat tgggcaccag aggcgaggcg atcaattcgg ttttccggta
     1801 gttccagccg gctagcgcgc cggcggcttt gtcgaatgtc aggacaaaat cttggccttt
     1861 gacaaggatg ctgccggtgt tttcttccac tgtcagagtc ggcattgtct ccagcgatat
     1921 ggcgggcgcc ggctgcggct caaacggcag gacaaactga tcccatgcca tcaggtggcc
     1981 tttgggagcc cagggctggt cggcattgag gacaaaacgg accgtcagca ggtattcccg
     2041 gccgggcttg atagccggtt tggcgaaggg aatcgttacc gattggctct gctgcggctg
     2101 aatgctgaca gcccccagag ttcctttctg aatgaccgtt ccgctttctg ccagttccca
     2161 ctgaacatcg acaaaatctg tcggaatgaa aaagtatttg ttgcggatgg ttatcttgcc
     2221 ggcagacagg tcatcggcac tgacggaaat attctgatag accttcttca tctcgtacag
     2281 atgcggattg ggcttgcggt cgggctgcac gaggccgttg cagcagaaat tgccgtcgtt
     2341 gggataatcg ccgaaatcgc cgccataggc ccagaaccag acatctttgg acggggtggt
     2401 tttcttgata tccgatggat taaagtccag ctgcagcacg gcggaagcat cgggtttggc
     2461 attggcggcg gcaatctggg catcggaaag aacggctttg taaatgcgga ccttggcaat
     2521 ttcgccgccg aagcgccgtc cgcggttttg ggtatcgatg ccgatgccga ccggatagtc
     2581 gcaatgggcg attcggccgg tgtaggtttc ggtgcctgcc agcttgccgt cgatatagag
     2641 tttcaatgcg cttccgtcat aggatgcggc cagatggtgc cactggccga accagttgtc
     2701 cggcaggtct gcatacacac tgtgccacgt gttgtcataa atgaaaaatt ccgcccgttc
     2761 gccgttgatt ttcagggagt actgctggtc gcctttggca ataatcggct gatgctgttc
     2821 ctgccctgcc cggaagggtt ttacccgtgc ttcgagggtc agggccgttc cggtgatatc
     2881 cagtgcatca gccggttcga cttccagata gccgctgacg gctttggcac cgccgggacc
     2941 ttctgtcagc ttgccgttaa gaaccgctgt cagcttggac gggctgaggt ctttaactgt
     3001 aaaaacggta tccggtttct tgcgcaatcc ctgatctacc cagtcccaga tggagccgcc
     3061 ctgcaggtgc ttgtactgct cgatggcgac ccagtagtca tgtatattgc ccagactgtt
     3121 gcccattgcg tgttcgtatt cacagagtat caggggacgt gtttgttcgc ggctggcata
     3181 gcggacgatt gtgtcaatgg tagcatacat cgggcagacg atatccgtat gtggcttttg
     3241 tccggcctgc tcataatgaa ccggacgcga aggatcccgc tgctttatcc atgcgctggt
     3301 ggcctcaaag ttcgggccgt cgccggcttc gttgcccagc gaccagatga tcacgcaggg
     3361 gtggtttttg tcgcgttcta ccatcgcctg cgtgcgggcc atatgggcct ctttccattc
     3421 gggcttcttg gctaacgact cggcgccgta gcccatcccg tgggattcaa tgttggcctc
     3481 atcgataata tacagcccgt attcatcaca cagatcatac cattccgggg catccggata
     3541 atggcaggtg cgaacggtgt tgacattgtt ctgcttcatc agcatgatgt cctgaatcat
     3601 ggactggcgg ctgacataat ggcccgtatc cgggtcatgc tcgtggcggt tgacgccttt
     3661 gatatagatg ggctggccgt tgaccagcag ctggccgcct ttgatttcgg atttccgaaa
     3721 accgatgcgg ctggacagat attccagcgt tcggccgctg ctgtcttgca gtgtcagcac
     3781 cagagtataa aggttgggtg cttctgcaga ccatttggcc ggattggaga ttttcaggga
     3841 aggacgacaa acagcagttg agccggctgc aacagcaatt ttgtcaacgg tcagcgaggc
     3901 ggttttgctg ccgtttgcac cgaccagctc aactgtcaaa ccagcggccg ccgggctgga
     3961 tgcaaaattg accagctcgg tttcgacatc cagtacagca tcccggtaat tgtcgtccaa
     4021 atccgttcgg acgaagaaat cccgaatgtg cagctgaggg gtcgcataca aataaacatt
     4081 gcggaaaatg ccgctgagcc gccaaaagtc ttggtcttcc agataggagc cgtcgctgaa
     4141 gcgatagact tctgcggcca agatgttgcg gccgggctgg agatattttg tgatattgaa
     4201 ttcggcagga gtccggctgt cttgactgta gccgactttc tggccgttaa tccacagata
     4261 aaaggcggat tctactccgt caaagacgat gaaaacctgc cggtctttcc atttggaggg
     4321 gatggaaaac tctgttcgat aggagccgac aggattgcgc atctgaaacg tggtgtactg
     4381 aaccgggggt gtatccatta cgcgcggggg gtttttggca aacgggtatc gggtgttgga
     4441 gtaaatcggg gtatcataac cgagcaactg ccagttggaa ggaacaggta tggtatccca
     4501 cgattggaca ttgtacaggg gcttgtaaaa atccactggc cggctgtcgg gatcaggcga
     4561 ccagcggaat ttccatttgc cgttaagcga ctgataccaa tcggacctgc tgcggtcggc
     4621 ctgaagggct ttgccggctg attcataagg aatcagtgtg cagtgcggtt tttccttgtt
     4681 gatgccgaat acagcgggat tttcccagtc gggaactgtc tgcgcgatag aaaatgccga
     4741 tgccatacag aggattccta tagcagctgc ggtagagaga acaatctgtt tcattcctga
     4801 atctcccaaa atgtcgtttt ccgttcgata aggcatttgt ccgattggta aaagtataac
     4861 ttaaagccct ccggattcaa gcgcatattt tgggagtttt acgatgggaa aagttcggga
     4921 aagcatcgga agtaatgacg ggcatagagt ccgatggtgc gcatcgcaaa aagcagtgcc
     4981 cccgccgctg ccagccgagc cgccagagcc gccagaatcc cggccgatga ttttatatcc
     5041 tgtgcgaaca agccgacagt tacataaacc gccagcaggg ccgcaaatgt gacagatgcc
     5101 gttagcagat aggccccagc tgttttggct atcatccgga tgattcggtc gtagcggaac
     5161 agctcccacg gggcggcgcc gctgcccagc attgccaaca tcatcggcca catcgtcagc
     5221 gacagcagga cgatagccgt ctggagtcct tgatgcgtca aaccaattat ttccagcagc
     5281 gcggtaatgg ctgatccggg aatggcggca gcggcagcgg cagcaatgaa caaataaata
     5341 tttttcaccg attcgcccag aaaagcaaaa cctgtaccga caaagatgtc cggcagaaaa
     5401 tcattgtcca taaaggtgct gttgatgatt tccatgcaat accacagcaa atagccggca
     5461 cagccgaatg tcacgatcca gccgacaatc agcggggggc gaaaatggcc gactgtaaat
     5521 gaataatcgg catttccggc aaagaagtga aatgcgatca aagcggcact caggataatg
     5581 ccaaacagac tttctttttg aatgaaaacg ctgaaattgt ccgccagtgc cgctcgaaaa
     5641 aaacccggca gcggttcttg cggcgacggc tggacgggta ccgcttttgc gccgtcggcg
     5701 gccggaatga taaagcgggc attgcaagcc agacagcgtg ctcttctgcc ggcatattgg
     5761 tccttgaagc cgcacacacg gccgcattgg ggacaagtgc acgagatcgt catcttagtc
     5821 gtttttcatt gcatcctgaa agctcttgag gtcattggcc catttctggg cttccagaat
     5881 atctactata ccctttttga ccagcaaggc cagatgccgc tccattgtaa tcatccgctc
     5941 atcgggctgg tctttggttt tcacctgcat ctgcgaatac atctgctcta ttttgccctt
     6001 gcgaatcagg ttggccagag cataattgct gttgagaatt tccattgcca ccacgcgtcc
     6061 tgtgccgtct ttgcggggaa tcagtttctg actgatgata tagcgcagcc ctaacgaaag
     6121 ctggttgcgg acctcatcct gcttctgatg cggaaagaag tccagaatgc gtgtgatgct
     6181 gccgactgcg tcgcgggtgt ggagtgtgga aaagaccaga tggcctgttt cggcagccgt
     6241 cagggtcagg gcgacggttt ccgaatcccg catttcgccg acaaaaatga tatccgggtc
     6301 ttcacgcatc atcgagcgca agccgtgggc gaaggtgtcc acatcgcgtc ccagctcgcg
     6361 ctgggatatc atcgaatttt tttgggtgaa aatgtattcg atagggtctt ccagtgtaat
     6421 gatgcggcaa gcgcggtttt cattaatgcg gtccagcagt gaggcaatag ttgtgctttt
     6481 gccggctccg gtgatgccgg taaacagtat caggccgtgc ttgcggcgca cgatatcctg
     6541 ccatgcattg ttgggaaagc cgatagtttc aggagccgga atatgcgttc ccagcgcccg
     6601 aatggcggcg gccagcccgt cgttgtccat aaacgcattg atgcggaatt gaaggctgcc
     6661 gaacttgtag gagcaatcca cggccaaatc gcgtttgagc gattcaatat tcttgacgcc
     6721 taacaggggg taaatcaatc gttctgcaat cgtctggctg acagcggctc ctttgagacg
     6781 gaccagttcg ccgtcgatgc ggtaggccgg cggagtgccg tttttgatat gcaggtcgga
     6841 gacgcgcata acccccattt tttcaaaata ggccagcata tcattaatgc ttaaaaaatt
     6901 tccttcgccg gcaggataac atttgtcgtc aattgtgtaa gtctgttcca taacaattgt
     6961 ttccatcacg agaaaaagta cggattagcg gctgtattgt agcaccaaag gtaaatgaag
     7021 aaaagaggtt ttaagcaggt atttttgaaa gttagtcggc ctgaacaaat gtataaacgg
     7081 agccatcctc gcagccgacc agcagccggt tttgggcggc agccggtgag gatgaaacag
     7141 agcctcccag cgtgtaggca aacagttctt tgccgctgtt tatatccagc agataaagcc
     7201 ggccgtcgct gcagccgaca gcggctttat cgccgcacag cagcggactg ctgtcgaagc
     7261 tgtcctgtgc ggcaaaggtc cacaggagct tgccgctctg acggctgaag caatagacct
     7321 tgccgtcgcg gcagccgacc acgacaaccc ggtcgttcac cgcaggcgag gaaaagaaag
     7381 catcttctgc cttatcaaaa cgccacagaa tagaaccgcc tgccgcatct gcggccaaaa
     7441 agacgccgtc ataattgcct gcatagataa cgccttcgaa gagcacaggg ttggccgcta
     7501 catatgagcc ggcgtcaatg gtgcgtgttt gttcgggtgc atcgatgggg acaagataca
     7561 gattggcatc gcaggagccg aaagcggcgg tattctgcgc agcagcaatc gccccgttaa
     7621 tgtagtttcg agccgggtac tgaaagacaa tctgacctcc ggcggcatcg acggcataaa
     7681 gattgttgtc ataggagccg aaaatcacga gagtttggtc tgtttttgga tgaacagcta
     7741 tattggctga gccggtaatt ttgccatcag ttttgcacga ccagacgagt tggccggttt
     7801 gagccttgac ggcgtaaaag gttccccgat tagagccgac ataaaccatg ccgttatgat
     7861 aaacagggct ggcctccaag gcctcatcgg cttcaaaccg ccagatttcc ctgccggtgt
     7921 gaagttcgag ggcatacaga caactgtcca ttgaggaaat aaaggtctta ttgtcagcaa
     7981 cgataggggt tgatttgacg gcgtttttgg tcttgaaagt ccagtcgggt ttgagtttgt
     8041 ccggcaaagt tcctgccgcc tgtcccgtca gggccgcatc gccccgaaag ataggccatg
     8101 cttgattgcc aaaggatgcc ggctgccgca cagcgggcgg caaaggcggg gcagggggaa
     8161 tgcgcttgta tggcagaata aaggcaatca tcgcaccgat tgcagcggca gcaatcagca
     8221 ggccaaacaa agttcgcctg attatttgtc tatagttgat atccattttc ctgtaatcct
     8281 ctgcgatgtt gtaagaataa tagagaaaac agccgcttcg gtcgagaaaa atcatatcaa
     8341 attcgttgca tttagacggc tttcggatac gataagcagt gtcagacaag cataaaatca
     8401 atcgaaagga accagacgat ggcggtaggg aaggcgtttt tcggcacgac ttcggacgga
     8461 cagaaagtag attgctttac actgaataat gcccgcgggc gaattgcaaa aatcataaca
     8521 tatgggggta ttgttaccga actgtggatg cctgatcgca aaggcgtttt ggatgatgtt
     8581 gtgctgggtt ttgataatct ggccgattac gagaccaaaa gcccctattt cggatgtatc
     8641 acaggccggt atgccaaccg tattgccgaa ggccgattca cgctggatgg tgtggaatac
     8701 agcggtttgg cgattaacaa cggagccaat catcttcacg gcggcatcaa gggctttgac
     8761 aaggttgtct ggcaggccga gccgttcgag aatgcggagg gcaagggcgt caaactgcat
     8821 tatttaagca gggacatgga agagggctac cccggcaatc tggatgtaac tgtaacctat
     8881 acgctgacgg atgctgacga gctgaagatt gagtatgcgg ctgttacgga taaaccgact
     8941 gtttgcaatc tgacgaatca cagttatttc aacttggccg gccacggtta tggaacccat
     9001 tatgagcatc aattgatgat caatgccgat gccttcaccc ccgtttggga tgcaggggct
     9061 attccaaccg gcgagattct accggttaag ggtaccccga tggattttac ccgcccgacg
     9121 cctatcggcc tgcgcatcga tgaaaattat gaccagctgg tgtacggcag cggctacgac
     9181 cataactatg tgctgaataa atccgatgca gcgctgtcac tggcagcacg ggtcagcgag
     9241 ccgacaacag ggcgggtgat ggaatgctgg acaaccgagc cgggagttca gttctattcg
     9301 ggcaattatc tggatgcatc gctggtcggc aaagggggca aaggctacgg ccgccgcttc
     9361 ggcttctgtc tggaaacgca gcattatccg gactctccca acaagccgaa ttttccttct
     9421 acagttcttc gtccggggca gaaattccgc agcaccacta tttttaaatt tctgacaaaa
     9481 taatggcttg cttaataaaa aagcccgctg gattattcag cgggctttct gttttcaggc
     9541 gtaggtgtgc aggcttcagc cgggttttat acgcatgcgc acggcagggt gcactcttac
     9601 tgtgtaagtc ttctgttatt actttatctc ttcggccatc cagccgagca gctctttcag
     9661 gtctgccaaa gtcgtatcgc ccatgtgagc gatgcggaag gtcaggtctt tgagcttgcc
     9721 gtagccgttg ccgaatacga cgttatgctt ttgctgcagg gctttattca cggctgcaat
     9781 gtctatcttg cgagtgtttt taaaggtcgt taaggtattg gatgcatact gcttgggcgg
     9841 cagcagctca aaaccgtgtt cctgacccca gttgcggacg tagtcggcca tttctttatg
     9901 ccgcttccag acattttcca ttccttcggc cagcagcttg gtgcactgat aatgcagcgc
     9961 cataatatgc gggatcgacg gcgttgtcgg cgtctggtct tttgcggccg acttggcaaa
    10021 ttcctgaaag tcgaaataat acccgcgttc gggaacggtt ttgctttttt ccaaggcacg
    10081 gttgctgacg gcaaacacgg ccagccccgg cggaatcgaa aacgccttct ggacagaggc
    10141 aaataccaca tcccagccca gcttgtcaaa ctccagcggc atgccgacca ttccgctgac
    10201 cgaatccacc agcaccagca catccggata cttctccttc atcatggcgc tgatttcata
    10261 gacgggattg gtcagaccgg tgctggtttc attgtgcacc agtgtcagga caggatattt
    10321 gccggtagcc atttttttat caatcatttc cggcgtgatg gcctgtcccc attcaactcg
    10381 gagttcttca acctgccgtc cgcaggaacg ggcaacatcg ccgaatttct cactgaaagc
    10441 accgcacatg gtgcacaaaa tggtttcatc tttgcgtacg cagttgcgga tgctgccttc
    10501 ccagatgccg gaggctgatg aagtgctcaa gaagacgctc tgttcggtaa acagaacctt
    10561 tttgagcatc tgggttactt cgctgtgaag ctgggaatac tctttgcggc gatggcccag
    10621 tgtcggccgg gctaactgct gcagcacatc cggcactaca tgtaccggac ccggaatgaa
    10681 taatttcggg gggtttttcc agtctgtcat ttcgaacact cctcttcaat aaggttagat
    10741 tgagtaaatt gaggttgaat gggttgtatg tccattttac gaatggttaa tccagagaag
    10801 attttggggt aaaaaaaggt agatttctga ggcatttttt cacccgcact ggcaacggcc
    10861 tgtacctgtt caatccgcgt gggattcata aagaatacgc cctgacttcg gccgctgtcc
    10921 acccgtgcaa tcgatttttc gatggcatcg ccgagatcct tgatgtatac aaggtgactt
    10981 tggctggcta atttcacatc gcagatgccc agatgctttt ccaaaataag cttatggagc
    11041 acattgacat ccaatttctg cgcggcctcg gacatatcgg gatacagccg gctcattgtt
    11101 tccgtattct tcagctttat ggtataaaag gcgccgctgc cggcgtagaa cccgaaacaa
    11161 ttgctgttcc tttgggcttc cattttccca aacatagctt tttgcgcctg ctgcttttgg
    11221 gccttattct gaaagggata ttcagcaatg tcaaattcgc tgccaaggcc gtccagcagg
    11281 gcggtgctgt caaaatcggg catatttacc agcagccgat gcgtcggctg aataaccaga
    11341 ccttcattgc gcatatttac aaaggtcatc atttgccagc ttgcctgctc attctgtgtt
    11401 tcggcccagt aattcaatgc cgtttcatat cggtgatggc cgtcggcaat aattgtttgc
    11461 ttgtttgtca tcatctgggc aaacatactg ataagctgca catcgctgat gacatacagc
    11521 cgatgccgta cgccctctgt gtcgatggta tcgacaaccg gcgtctgctt cattgctgag
    11581 tcaatgacag tccggtccgt attggccggg tcgtcataaa gcataaatat ctgacccagc
    11641 tggcaggcgg tggcgcgtgt cagattcaac cggtcggcct tggggccttc gagtgttttt
    11701 tcgtggggct gaaccccgcc gccgtaccgc tgcagtttgc ccaaggcaat gattccgctt
    11761 cgttcgagcc gtctttgacc gatttgaaag tcctgaacat aagcataaac tgcatcatac
    11821 ggctcggcta tgagggcttt ttcggcgaag gctttttcca aaaactcggc cgcacgggta
    11881 tatacattgc tttgcggtgt gtcatctgga gaggtttttc cgcgtgtagc acggacaata
    11941 ttccaaggat ttcgttgatg cagctgttcc tgcatagctg cgtcaatgac atcataagga
    12001 ggagcaatac aatcgccgac attgcctact acttccggat taaatctata tgctctaaat
    12061 ggtttaatat gcataagtta taaatattct tgctgagaaa gtgccggttt tttaatgttc
    12121 attcaatcac aacaaaactt catttcggta caaatactta gagtaatata ccacaaagga
    12181 atgctaaatc aaacgaatta taacattttt ttacattatt tgataaagtt cgatattttt
    12241 aaattgaccg tttattggct gtacaaaagg ttgtttttaa aataaaaaat ggatacaata
    12301 caaagaaaat ccgtattttt tgggattgag gatttagatg tctgagctcc agaccgttat
    12361 aatagaggat cattcttgca caatggggag agaggggatg aaaaaggcct ttacactcat
    12421 cgaattgctt gtcgttattg ctattattgc gcttctgttg tccattgttg tgccgggttt
    12481 aagaaaggcc aaagataaaa ccaaagatgt tatctgccgg gctcatatta aaggaatagg
    12541 cgtagcggtt ctgctctatt tgagcgacaa tgaatcccgc gccttcaaca atcgggaatc
    12601 caacggacac ctttggtatg atgcggcagg caaaatcatc acgccggaca atacagtctg
    12661 gtgggcggat gcttactggg gattgggcta taggatgtac gcttcggatc cgagagtttt
    12721 cagctgtcca tcgtttgtgc tcaaagatgt ggccgggctg atgtactcgg accatccaaa
    12781 ttataaaaca acacggacag atttaaccag agcttcgggc tatgggctga acagcttctt
    12841 tttccgggac ccggaggccc ctgcatcgga tgtataccga aaccacagga aaatcagcac
    12901 tatcagatct cccagccgct ttatagtggc acatgatcat atggagccga agatggaggg
    12961 caattccagc ggcagcgagc agggcgatac gctgtatgtt ccaagcggaa gcacatttaa
    13021 tctcgttcag tatcgtacag gtacccgcca gcccttttac ggcggcattt tcaggcattc
    13081 caaaaagagc aagaactggg atgaaccttc ccagctggca gcacgattgg cccagataga
    13141 taaaaatcca aacggcaatg tcaatgtcct atttgctgac ggcagtgttg acaaaatcag
    13201 tgaaacaacc ggtcagaata ttccctacac gatgtacagc ggtctgctca aatagaagcg
    13261 atacggctgc cggatgggat tacttgacct gaatgggcag attggcagtg gccgttttgc
    13321 ccgatgcatc acggatgtaa acaaaaactc ggtaatttcc gctttcctga ggcattctga
    13381 ttaccgcacg atccgcttcg gaggagacca ccgctccgtc tatgggggga acgcgtcttt
    13441 cccagtcccc gccgacgctg ggattgtctg attcatcttt gcgaatgtcc cattcagcag
    13501 tcatctggtc tccttcggga tcgtttgcct gaactctgaa aacaacctct tgcgagggcc
    13561 tgtagatatg gtgcatctca ttcccttcga cccagatttt tttgtccccg atactcgggc
    13621 atttgttggc aggccatttg cctgtccaca ggtatgtcat cgtatccatc atttcagtgg
    13681 gggtgccgtc cggcagaaac atattgaacc atgtgtgcgt tttttcctgc ttatttcccc
    13741 agagaaagac ataagagccc agacagttgg gcttgtcctc aatagcggct ttgtatgcct
    13801 cgaaataaaa ccgggctttt tctgtgctgg tttcctcgat gggcagtccc caagctgtgc
    13861 ggggtgcctc ccaccagccg cgcggcccga actccgttat tatataaggc ttctcccagc
    13921 cccagcggcg gatctcagaa ggtatgaagc tcaatgtccc atagctgttg atgccgatgg
    13981 cgtctaatgt cgggcagagc tgcttaatgt cttcaagttt cttgcccgca ccggctataa
    14041 ctgtaataac gggatgatgg ggatcaatcg ctttaatgtt tttggcaagg atttcgattt
    14101 ctttccagac aagaatgcag tcctcgggct gggccagatg ctcgacttca ttaccgacag
    14161 cccacagcag aagggcgggg tggcttttca gttcttcgac ttctttgcag atgcgctctc
    14221 tttgttcttg aagccatttt tcattgcggt aatcggcgcc gtgacgaggg ctttgcattg
    14281 acagacccat gcagacggtc aatccgtacc ggccggcact gtccagtgct tgccgggagc
    14341 tgctccatgt ccgtatggaa ttgcccccgg cttcaaccag cttttccaaa tatctggctc
    14401 cgccggcacc tttgataaaa tagggcttcc cgttcagcag cagttcaaac cgctctcccc
    14461 gccgctcaat gacagccgtc gaatgagtgc tttggatgga tagcccgggt gtctgtgaat
    14521 tgccttctgc ggcagacggt atcagcataa gcaaaccgat gaataaaaag aatagaatgt
    14581 ttgttgtgtt cataaagata tccagaaaca gttcctcatt ttttaattag agtatctata
    14641 ccctcctgtg tcaacacaat tacaatccag caataattgg cacaataaat ccttaacaat
    14701 ctcaaaacac ctgaaaatgc agtgtcttgc aggattttta tgcaatagtt ttccgcagca
    14761 ataattattc ctaaaaaaat aaacattctg cgtttggtta tcggctgggt atttataatc
    14821 cgactctata aaaaaactcc agcaaccgtt tgctaatttc ggaaaataaa tataaataat
    14881 agtttaagtc ttgtttgact ggggtgaatt tgctataatc ctgataatgg ggcgccggta
    14941 atgttccctt attggaataa gtatcaaaat gaagcttatc ataatagtct gtttatcggt
    15001 ttctgtgtcg gttttgggac agaatctgct tattaatgga gattttgaat cgggaactgg
    15061 cggatggaca aagtggtgga acagcagcaa tgctggtttt ccagtcagcg atcctgttga
    15121 aggggataac tgcgccggag tctggtggtc ggatgtcggg atataccaac gtctttcaat
    15181 cggccccggt ctttatactg taagcggtca gatgctccac acatcggcgg ctccgctggg
    15241 caacaatcgc atcggtatta ttcaggccga agtgggcaat ggaacacaga tatggtggaa
    15301 acagcagatt gtgatagacc aaacctctcc ccgtgacaaa tggatagacg gccagctgat
    15361 tgtcgacaac cgaaccgccg gagcttcctc tctaaacatc aatctgttta tgtatgaccg
    15421 cgacggtgaa cataccggca acggaattgt acgctatgac aatctttccg taacgcctga
    15481 ggctgcggtc aatgatcccg actacaacga tgacaaggca gtgaatcttc tggatttttc
    15541 ggagttttca ggtcaatggg cgctggaatc gctgagctgc aatttaagcg gtccggctgt
    15601 tatcgacatg gatgatttgg cggtatttgc tgaggcatgg ctgatggaca caccgaccta
    15661 tccgggatat gagctggtct ggtcggatga gttcagcgga tcatcgctca atctggccga
    15721 ctgggaatat atgattgggg atggatgcag ttacggagta tgcggctggg ggaataacga
    15781 gttggagtat taccgggcgg aaaatgtcag tgttcagaat ggtatgctga taatcgaagc
    15841 aagacgggaa aattatgggg gcaagcagtt tacatccgga cgcattcgaa ccgccaataa
    15901 gcgggatttt ctgtatggga gaatggaagc ccgcatcaag gttcccaccg gcgggggaat
    15961 gtggccggca ttctggatga tgcctaccga cagtgtttat ggcggctggg cggccagcgg
    16021 tgaaattgat attatggaaa cccgaaacag caccaatttt atacaaggga caatttttta
    16081 cggcggcggc tggccgaacc agcaaagttc aaccggcaaa tatgagccgg ccggtgtaga
    16141 tttttcgcag gattttcatg tttatacgct cgaatgggaa ccggatataa tgcgctggta
    16201 tgtagacggc attctgtatt cgaccaaaac cagcagccaa tggtacagtg acggtgcccc
    16261 cggcaacaac cgggcgccgt ttgaccagcg tttccatctt cttctcaata cagccgtcgg
    16321 cggcaactac accggctgca caagtgcatc ctgcattact gcggttttcc cgcagcagat
    16381 gcttgtggac tgggtacggg tttatcagaa aactgcacca tagtgccgcc ggcaaacata
    16441 tatccaaaga ggcctcaaaa cagcggatag atgaacggtg caattgcggt gctgcttaga
    16501 agcagcagta aaccgattgc cagcagcatg aggataatcg gcaaaagcca ccatttctta
    16561 ttgtgtttta gaaattccca gaattcgcgg agaagcgttg gacgtttttc acgggccatt
    16621 tgttcaaaat tggtttcggc tgtcatggat tattcctaaa attggcggaa atagtctttt
    16681 gcagttggtt ttttggggcg gggtatccaa taagtctcta aacgggattc gtcgtatcgg
    16741 cgcagcgggt gatttcccag cagccgccgg caaagaccca ctggggtaaa aatacaataa
    16801 aaaaagaatg ccaaaatcat atgactcagc agccatccga tgggaaatcc cagcagaatc
    16861 atcactctgt aaaccggttt tgcccataag ggccgaatca gtcccagacc gcacacagcg
    16921 gcgccgagga aaaatataaa caggcattga acgcctgttg ctcttgccgt tttgaacaaa
    16981 atcaggctgg cggccgctgc cgctgtcagg caggcaaaac cgaacgtccg aagctcttta
    17041 tcagaagggt ttaaaggggg ctttatcaag gtcatgctgt ctaatccgga ttaacaagga
    17101 caggatagtt gccggctgca gacaggaaag tctggggctg ttcttttttg tacaaaatga
    17161 agttctctat ggcaagcaca tccatatcgg tatggataaa gcacttcaag gcatcgtcag
    17221 gagtacagac aatcggttct ccgcggacat taaaactggt attgataacg gctggacatc
    17281 ctgttttctg gcggaattgc ttaagcaggc ggtaaaatcg gccatgccgt tgggggttga
    17341 cggtttgaat gcgggcgctg taatccacgt gcgtaacagc cggcagcatg ctgcgcttga
    17401 ccttcagttt atcaaggccg cagcagcttt cctgctgcgg tgtcagagga atgcgatgct
    17461 caggccgaac ggccgctgtc aaaagcatat aagggctctc ctgacaaagc tcaaaatact
    17521 cggcagcgtc ttcctgcagg atgcttgggg caaagggccg gaaagactcc cgaaacttga
    17581 ctttcagatt cattcgggac tgcatgtgtt cattgcgggc atcgcccaga atgctgcggc
    17641 ttcccagcgc ccgcggtccg aactccatcc gcccctgaaa ccagccgacc acctgtcctg
    17701 cctcgatggc ttctgccacg cgggccagca ggtcttcttc ttggtcgaaa aagcggtact
    17761 ttgcccctgc tgcattcagg cgggaacgaa cgtactcgtt tgagtaagcc ggtcccaaca
    17821 gagagccctc ctgctggtcc tgctggccgt cggcgatccg cggattgttc agcagctgat
    17881 aatgaacaaa cagcgctgcc cccaatgccc cgcctgcatc gccggcggcc ggctgtatcc
    17941 agagattgtc aaacggtccc tcgcgcagga tgcgtccgtt gccgacgcaa tttaatgcca
    18001 caccgcctgc catcaccaga tttttcattc ccgtcttgca gccggcaaaa cggacaatcc
    18061 gcagcataac ttcctctgtt actttctgta tggaagcggc gatatccatt tcccgctggg
    18121 tgagaggaga ctccggcttt cgggctggac cgccgaagag ttgggcaaat tttttcccta
    18181 tcatataatt ggtatagccg taggtaaaaa aatccatatt cagtcgaaat gagccgtctt
    18241 ccttcaagtc cagaaggtta ttcaaaataa ggtcggcgta aacagggcgt ccgtagggag
    18301 ccagccccat cagtttgtat tccccggagt tgaccttaaa gccgcagaaa aaagtaaatg
    18361 ccgaatacag caggcccaag gagtggggga aatggagttc atagagcaga tcgatgcggc
    18421 tgtcttttcc tattccaatg ctggcggttg tccattcccc gacgccgtct aatgtgacga
    18481 ccgcagcttc ttgaaagggg ctggggaaaa atgcgctggc tgcatgtgct tcatgatgtt
    18541 ctgtaaaaat aaaagacttt ttaaaaccgc cgcccaaaac cttgtccatg tgccggggaa
    18601 tctgaagctt ctgatgcagc cattggggaa ttgcggttag gaactgtccg aagccgcgcg
    18661 gggcatagtc cagccatgtt tcgagaagcc ggtcaaattt gatcagcggt ttttcataga
    18721 atacaacata atccagatct tccggcccga tgtgtgcttc gctcagacaa tagccaatgg
    18781 cattggtcgg catgtcggca tcatgttttt ttcggctgaa gcgttcttct tgagctgctg
    18841 ccagaatccg gccgtctttg agcagggcag ccgcactgtc atgatagaat gccgaaattc
    18901 ccaaaatata aaccggttta gtcatttgtt ttctgcctgc ggctgaggcg tgtttcaatg
    18961 tggctcaaaa gcgatttgaa taatttatct tccgcaaacg ccggatttat ttccaaggcg
    19021 attttcattt cttcaagggc cttttcatag ttgccctgcg agttgagcat ataacccagc
    19081 gtggcatggg cggcggctgt ctgttgccgc gggaaaatag cgaccgccct gtaaagcgtt
    19141 tgaacagctt tgttgagttc tccggatttt tcatacgcct cggccaatgc gccgtaggct
    19201 tcgatggaac cggctggatc aaaccagata gctttggata aatgcttgat atagttggag
    19261 gtatcggcaa tctgccgata aatcatcgcc agttccagat gggcttttcc gtcgtagccg
    19321 gctttcaggt ccagcagccg gcggagtatc tcctgcgctt ctttcaattt gccctgctga
    19381 tgaagatttt tgcccaaact gaggtaggcc gcatcgtatg ggcagagccg aatgaccttg
    19441 cgcagggctt cttcctgtgc tttgatgtcg ttgagacctg tgcctgcaaa aacggcatat
    19501 tgccacaata tctgccaatc gtccggccgc cgctgcattg cctgtgtgta ctgccggcgg
    19561 cagtcggata tgccggcctt ttgcagcatg gtttcaagct gccgaatttg ctgctggacg
    19621 gtctgcatga actccgcatg gtaaagctga ttggtaaaag gcggctggct gtacattttc
    19681 tgatagagac ggttcagaaa atcatgccgc tcaaaaggag tgtaagccag ccgctctgca
    19741 gcctgctgtt cgcttagaac agctccggcc gaagatgccg gaaaacccga caggcactgt
    19801 tgaacggccg gcagaagggc agttgccagt acataattgc ctgtgaagtt cagatgcaca
    19861 tgttcgtaaa acaactcgcc ccccggcgtt tgctgcgggc tgtgttcttc gagggcggcg
    19921 gcaccgtcaa caaatacaat gcctgattgt ttaaattttt cagcggccga gcggataatt
    19981 tgattaatcc gtgcatctgc ccgaaaccgc aatgtgtcat attccacagc gctgcggtaa
    20041 tggtttttgg cggctgtgaa attgccggcc agccaatagc agcgtcccag ccgaaactgc
    20101 aaatctgcaa aagtcgggtc aatttgttca gccgcatggt atttttcaac agctgactga
    20161 tgctggccgg ctgtttcata atcaatgcct tcttggtaca gcgtttccca ttcctgcagc
    20221 tgggcctttg tgagatcttc ccgatgcagc gatgcaaaag gagggcaatc tttgagattg
    20281 gcccccacgg ttgaaataat gacaggaatt cgccggcggc cggcaatgct gcagatgtcg
    20341 gtgagattct gttcgaaatg acgatagaca tagtccattg cagggctgtc gtagcgaacc
    20401 tgtttgccca gaaacatacc cagtcctccc catcgagacg gggccggatt tctgaattta
    20461 gccgaatcga gagcctgctc taaaagctgg cctgtccgtg tcgatttaac ggccagattc
    20521 gcccgaataa gcccaagact ggacagcagc ggcgaaaaaa cagttcccgg tccgtacgga
    20581 cccaccactt cattgttgcc catatacagg ataagcacat ccggattata gcgggtgcat
    20641 tcctcagcaa ttttcaaaac ggcatgcgag ttgattgcga ccattgcggc tgtatgaact
    20701 tcaaaccgca caccgggata caattcctcc agcattactt gaagaattcg cccaaaattg
    20761 taagcgggag cgggcattcc catggcggcc gattcaccca aaacaaaaat acggcaggtt
    20821 ttgtgcggtt tttcggcctt aaatacaaat ccgtcaaaat tgcgggccat ccggcggggg
    20881 aagaactgcc agccgaactg gtagttgtga cagcagaatt cttcgccatt gagggtgcat
    20941 ttgacgattg ctttggttga ataaccccag cccagtaatc gaagtccgca ttcccagatg
    21001 cccgcaaaca gaatagggcc gacaaaaacg cagataaggc gaaacagcca gagacgtgct
    21061 ctgctggagc tgcccgctga tgaagcaata gataaattct gttttgccgg cgtctgtttt
    21121 ttcatatacc tattgtatcg gatacgacct gtcaggaaat tgaataattg ctctctgcac
    21181 cggcagggct gagggcgcag cccaaaagtt taccacgtcg gctgacaaga gctgttgtga
    21241 ggatcgttgc actccagcca gtagagacag aaggcttccc aatctgccaa tgttacggaa
    21301 ccgtccaatc ctgcatcggc cttctgacaa tacccgttat ctggaccgca gttggcatca
    21361 agccagaaag cagcccatac aataaggtcc tctatatcaa cggaacaatc tcctgatagg
    21421 tcagcgggca gttcgaagcc ggctgcgtaa acttcggagc agttttgcgg gtcgggattg
    21481 ctggatgttt ccgtcagtgt aattgcgtca agccaaatcg tccgaggggc actggcgccg
    21541 agcataaagg cgagattgac attgtctttg tccggcgaga tgccgcttac cgtgaatgtt
    21601 tgtggtaccg gcgttaagtc ggccgtataa ctgataaagg tgctgtaggg tgaatgggtt
    21661 tcctgcaggg cgacgctgat agatgtgttt ctgtcggctg cggcttggaa tgtcagcgta
    21721 taccggaatc cggcttgggt attgagattt tggtaatact gcagatgcca agggatgccg
    21781 cttactgaat taatgattag tttgcaggag ttttcccctg agagtttaga cgtcgaatcg
    21841 atggaacttg acattgacag tccggtagca ttccatgctg ttgcccacgg gcctctgccc
    21901 tgatcgaact gaccattgat gaccttgtca atactcggcg ccgcaaccgc cataattggc
    21961 tcagagaccg gaccgcggtt gccgcccttg tcaatggctg tgatttggta gcggcaggtt
    22021 ttgccggcag caacagttgt atcttcaaaa taatgctggg tgttgtagcc ggccaaaatg
    22081 ccgtcccgca ggatttcata ccggttaatg ccgacatcat ctgccggggg ctgccattgc
    22141 agcaaaattc catgttgggt ggcagtgacg gtaatatcat ttatttttgc cggcgggata
    22201 gtgtcgatta tgcctgtaat atccgctcgc agcgaaactt catctgttcc gtgcaggtac
    22261 agcgccgagt ccatctggtc cttgtaatgc tgggcaacaa tcggatacgg ctgcaggcgg
    22321 cagtcgcccc actggccgcc ccaattggta ttgatatagc agaaattttt gattcctgcc
    22381 cagctgttta ttagattaaa ataaggcacg aaatcgccat tccagctggc ttcgccttga
    22441 tctgtatgcc aaaagacagg gctggcctcg ccgatgagaa caggtttgcc ccgggcgtgt
    22501 gcatcattga gaaaaagggg tgtataagga tgctggatgt tccagacgcc gaaggcattt
    22561 atgctcatcc agtccacata cgcatcgccg gggtaccagc tctgataggg accttgaatg
    22621 acattgaaaa ccgtggctgc ctcaacgttg ttgctccgca gagcgttggt gatacgtatg
    22681 aaagcggctt tataggcaat cgggtcatag tggttatgat cgccgttgca ctcgtaacca
    22741 atgcggatat agagggggta tccgagctgg ttgaggtctc ggcagaaacg ttcaatatcg
    22801 gcatcccata agccggcagc gatttcattc tcaatgccga cgatataaag ccccagctgc
    22861 accggcagat agactccata atcccgccgg tatcgttcca gctccgcccg
//
LOCUS       DAOTBO010000037        22780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1863_length_22780_cov_4.689329, whole genome shotgun sequence.
ACCESSION   DAOTBO010000037 DAOTBO010000000
VERSION     DAOTBO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 22780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22780
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1863_length_22780_cov_4.689329"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLE88_06075"
     CDS             <1..780
                     /locus_tag="PLE88_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76066.1"
                     /translation="LFGQILHGECGYLHDLRALNLSPTYYQGMWRVYHYIDMDGNLYP
                     THGIGPMAWCMDINRGDTLDYLVCFSSPSVGLQEYAKAHYPDSRWARQEFANGDVATC
                     LIKTKLGKTIICKHDTSLPRPYSRDFLVQGSKGLIQKYPQPVIHIEGRSPEHAWESFD
                     GYLQEYEHPVWLNLRKKTENAPDSGLGHGGMDYIEDYRLIQALRQGIAPDIDVYDSVL
                     WSAIIPLSRQSLAKGSAPVAFPDFTGGAWKQKRPLGVDRIL"
     gene            738..3182
                     /locus_tag="PLE88_06080"
     CDS             738..3182
                     /locus_tag="PLE88_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76067.1"
                     /translation="METETPAGCGPDSVSEQGEVMAGRHSTKTAKPLNRVALSKVDQY
                     ALTLSDYDCLYPPAEKIPAIVVPHFPLLGKLTALRFLEWVQQHPGGVVSLPTGKTPEF
                     FIKWVRRFLRDWDKPAVQAELEQAGLDPSVRPAMKSLYFVQIDEFYPIHSEQTNSFSY
                     YVKKYYLKGFGMDPAKAMLIDPCRIGIPNCRQLDAVWPDKYVDLSLRCRSPKNKLEQQ
                     QKQLLERIDQWCMDYESRIRQRGGIGFFLGGIGPDGHIGFNISGSDHYSTTRLCATNY
                     ETQAAAAGDLGGIEIARKCHVITIGLHTITSNPDCTAIIMAAGESKAAVVAKAIQSDP
                     DVKYPATALQKLPNARFYLTQGAAKLLVQRHYQDVCTASQLSDSQVEQILAELSVQKR
                     KPIIRLTPHDYQSNPFSRQLLKKRKETVAQLNQLAADNLERKIAKGMESLENTCFLHT
                     EPHHDDIMLGYFAQIARHFRKASNRHYFLTFTSGFTSVSNPFMVRQAVNLQRFVHSEE
                     FAYLYRQGYFDPTNQMARNRDVWQYLDGVAASNADMKNEGCARRFLRNLFELYPVKSL
                     SGIDKILTDLLEYFRTQYPGGLDPHHIQLLKGRCREWEAECLWGYYGWNCDAVSHLRL
                     GFYTGSIFTPQPSRRRDVLPIVRHLQRVQPDVISVALDPEGSGPDTHYKVLQATAEAL
                     RIYQQQTRRTDIRIWGYRNVWYRFDTSEANVFVPVSLNMFSVMHQSFENAFLSQKEAS
                     FPSYEYDGPFSELAQQIQVQQYQTLKTCLGREWFYQHRSPLIRATRGFVFLKEMEPEE
                     FYRSCRKLRESIEDIS"
     gene            3193..4146
                     /locus_tag="PLE88_06085"
     CDS             3193..4146
                     /locus_tag="PLE88_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76068.1"
                     /translation="MAFQPTLLILAAGMGSRYGGLKQIDPVGPHGELIIDYSLWDALR
                     AGFRKIVFVIRRSFEQAFREAVESKIGSRAETAYVCQELDCCLGGFELPAGRQKPWGT
                     GHAILTAQEMIHEPFAVINADDYYGPAAYRIMAEQLQTIQTRPHEYAMVGYILRKTLS
                     ACGAVARGICRHDSDMYLTAIAECTGIRPQGRQAVYADAQGHEHMLSGDEIVSMNLWG
                     FAPDIFAFLQQQFSAFLSRCGRDNKSEFYIPAAVNTLVQSGQKKVKILTTPDSWFGVT
                     YRGDKEMAQACIRELISRQVYPDHLWEHSVQQPHTLSGETK"
     gene            4143..6098
                     /locus_tag="PLE88_06090"
     CDS             4143..6098
                     /locus_tag="PLE88_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOM76069.1"
                     /translation="MNRAVFCAALLTAFSHTLPAAPAVCIIPQPVQLEMHQGSFEMTP
                     KTVIAADAPLRPLVEQFQQMLQPALGYLPAAVSSADRTNGSCILLECLPELDSLGPEG
                     YRLLVKPDRVVLQAPQQAGLFYGIQTLRQLLPSEIFSRQPVPDRRWTIPCVEITDYPR
                     FKWRGLHLDVCRHFMPKEFVKKYIDLLAVHKMNTFHWHLTDDQGWRIEIKKYPKLTAI
                     GAWRKETVIGKNTATYDGQPYGGFFTHEDIREVVEYARQRHITVVPEIEMPGHCLAAL
                     AAYPELSCTGGPFEVRTSWGIEANVFCAGNDQVFQFLEDVLDEVLALFPSPYIHIGGD
                     ECPKERWKQCPKCQARMKAEGLNDEHQLQSWFIKRMERYLASRGRRLIGWDEILEGGL
                     APGAAVMSWRGEQGGIAAAKAGHEVVMAPNSYAYFDYYQADPKTEPLAIGGFVPLEKV
                     YGYNPVPDVLSAEQQGFILGVQGQIWTEYIANGDYAEYMAYPRACALAEVAWTPPAQK
                     EYEAFYQRLQEHLKRLKALQVNFRPLDPLRTIAGSWKSGQTGQTFAPLQWNVSTVLKE
                     AGTYEVAFQYTGGAHRLDIQSVELLAGDTIVASDYHFGITGGRTQDNVYRLTVRQYDP
                     TAAYVLRAVVRSDGGTDSNGYVYLTKK"
     gene            6119..7231
                     /locus_tag="PLE88_06095"
     CDS             6119..7231
                     /locus_tag="PLE88_06095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOM76070.1"
                     /translation="MTADFADIVKAFRVEGILDSVTPCGSGHVNDTYIVACRQNGQHR
                     RYTLQRINHSIFTHPQQLMDNVLRVTQHIRHKLQQEQAADIERRVLTVIPTHSGGSCC
                     QDCHGCYWRMYQYIDEAQSRDYLSSPDMAFEAARMFGRFQRLLVDLPEPKLAETIPDF
                     HNTPKRLADFIRALESDSHNRAAEAKEEIRFVLDHAGMCDHLLRQVRQGLIPERITHN
                     DTKISNVLFDSRTGQGICVIDLDTVMPGLSLYDFGDMVRTAACPAAEDEENLSKIRLE
                     RTFFAALVRGYISQTADFLTPAEKQHLVFAGKLITFEQMIRFLGDYLNGDSYYKISRP
                     GHNLIRARTQMKLVQSIQEQEEALHACVNSLLETVQ"
     gene            7269..9539
                     /locus_tag="PLE88_06100"
     CDS             7269..9539
                     /locus_tag="PLE88_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOM76071.1"
                     /translation="MTLALIDWLIIGSFLATVIVIGLIVSRKGSRDAAAFFVGGRSMP
                     WWLLGVSMVACTFSCDTPNLVTGMVRGGGVAANWGWWAFLITGMVTVFIYAQLWRRSK
                     VMTDLEFYELRYSGKSAAFIRGFRSLYLGIFFNCLIMGTVTLAAIKIGQVIFDIPPWA
                     AVVLGSVGVVVYATAGGIQSCIWADFFQYSVAMFGAVYAAVVACQQPQVGGLSGLLSN
                     PNVLGRLSWMPDFTTWLAWVPLLLMPVAVQWWSVWYPGAEPGGGGYIAQRMLSAKDEK
                     NAIGATLFFNFMHYAMRPWPWIIVALASLVVYPSLDSIKADFPGIDPSYLREDIAYPV
                     MISKLGPGLLGLVVASLIAAYMSTIGTHLNWGSSYVVNDWYKRFMRPQAGQKELVLAG
                     RVCTVVLMLIAAFFALTFLEDANQAFEILLLSGAGTGGIYLLRWFWWRINAWTEIAAM
                     VAATIAAIWIVLGTGDEGTLKWYVDWAAVRDYVCVIDDAAVVDSVTAQQKQLLAQTEE
                     VRKELDQRIAAWRRARRGDASARTAARTEYEAALDRLHRIQQAASIPLLVQAAADESL
                     QPKLQQFYHQQKLWELQREKKRLSRTGDGAPRSIEDITISFGGVVKNGKIYLFHPLLN
                     NLKFSVQLLVCVGLVTIVWLITTCITKPADPHTLRRFYKLCHPGGPGWRKVVQDAKAD
                     GEEIDSKNAVGDWKLPMQLVCVFLGCVAIYASLFAVGSFIYGHRIWGFALSAAALAAV
                     VLLFKSFSKIGVESES"
     gene            9550..10221
                     /locus_tag="PLE88_06105"
     CDS             9550..10221
                     /locus_tag="PLE88_06105"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOM76072.1"
                     /translation="MNNRYAVKRCSQAVSIDADWNKSCWQTVEPLSVKVPNWQPAGSQ
                     PLPQTEVKVQYDDAHLYVIFRVSDYYVTALTTQTHGPVYKDSCVEFFFSPACGRPDSY
                     FNIETNCCGVLLAQHHTGPRQNSRFLDIADCRQIQIAGTVSGPIRQEIAKPMTWVVEY
                     AVPLEILLHYTAIDKPAPGVCWRGNFYKCADGCSRPHWISWLPIPSDIPDFHRPDCFG
                     LLEFM"
     gene            10354..12327
                     /locus_tag="PLE88_06110"
     CDS             10354..12327
                     /locus_tag="PLE88_06110"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOM76073.1"
                     /translation="MQILSACLWAVQPAMFYTSFEEFAPGEFTTLENALGTWTAAQGK
                     ASVSSYYRSGRQCLHLAGGETAVELVLPADRPEFSHLSFWAERFTAQDPFQFRILAGS
                     QSGWKEIYNGDAAIKTGRGFLSWVKVGLPEQNCSALRFVCTAPPGKGILIDDLQLHRP
                     AAMTLQNVTTAQWVGPVLLRKPHNPVLAVKVVTEGVLNPLTVTSLTITPEGTTNTSAI
                     ESFRIFYTGTEERFSTDSAFGSAQNPSDTMFFAGRQVLQEGVNYFWVSCTLKASANLF
                     DKVDASCIQITADGQNLVPNPASGPIAKRIGYNVRDAGDDGVRGYRIPGLAATLRGTL
                     IAVYDIRRHGMGDLPGDIDVGMSRSTDGGQSWEPMKVIIDMGPPHDQNGVGDPAVLVD
                     KTDNTIWVAALWSKGNRAWSGSGPGMSPDETGQFVLVNSRDDGLTWSEPIVITPQIKD
                     PKWRLVFQCPGNGITLRDGTLVFPAQFKDENDVPYSTLVYSKDHGQTWNIGSGAKSNT
                     TEAQIAELNDGSLMLNMRDNRGGWRSVCVTRDLGRTWQEHPTSRCALPEPVCMASFIR
                     FSSVLDGDTRNILLFSNPATAGGRRNMTIKASLDEGMTWPQQYHTVFYEPPCAGYSCM
                     TRIDKHTVGILYEGGDSALLIFEKFPIAEIVPE"
     gene            12429..13601
                     /locus_tag="PLE88_06115"
     CDS             12429..13601
                     /locus_tag="PLE88_06115"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOM76074.1"
                     /translation="MEFKDLAEQYRTELFDNVIPFWLKYSPDPVYGGYFTCLDRKGQV
                     YDTDKFIWLQCRQVWLFSMLYNRCRKEPLWLDTAKQGIEFLIRHGMDSEGNWYFALDR
                     TGQPLVGPYNIFSDFFAAMALSQYGLAAQDSRCTDLALQTYQNILRRKDNPKGRYNKQ
                     IAKTRALRGLGFPMIMANLALEMEHVLSADELNRILDICIREVMGLHVDKKRGLVFEN
                     VLADGSHLDCFDGRLLNPGHGIEAMWFLIDVGLRRQDKTLVEQAAEVILNTLTYAWDP
                     AFEGIFYFMDSQNKPPQQLEWDQKLWWVHLETLVALAKAYRATRRSACLEWFNRVHHY
                     AWSRFADPQYGEWFGYLNRRGEVLLPLKGGKWKGCFHVPRALWLCYKEFETLQAEV"
     gene            13645..15711
                     /locus_tag="PLE88_06120"
     CDS             13645..15711
                     /locus_tag="PLE88_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOM76075.1"
                     /translation="MTTAFKIFCSLQTAVFFGAFLLPALSVSAAEVSPVQRAKAPASL
                     IPYPQQVQWHSGQCILNTCTIVHQPSTASPQMSDIVNSLVQILQSAGSKVSTEGNAAY
                     PIWLVLNEQQPNQAAEAYHLLVTADKTVVTASKPAGLFYGVQTIRQLLQQENGRIFLP
                     CCEMTDWPAFPYRGFMHDVGRNYQDIELLKRHLDIMAHYKLNLFHFHLTDDPGWRIEC
                     RMYPQLNEPQNYRPTRQPGKYYTYEQINDLIAYARQRCIQIVPEIDTPGHSEYFKKAF
                     GFEMQDAEGIAVLKNILNEFFEQVNTPWFHMGSDEVQLKNPAFMKEMADFIRSKGKTA
                     LVWRPGHLPDTHVITQLWTGQSRPVEGVPYLDSQANYINHMDPLVGPIRLFFQQPCRT
                     PAGNDMALGGILCHWPDVNISSQMDIYTQSPVYPALVTYAERIWRGLPANREDCWAKL
                     PPAEDPGFQDFAAFEKDLIVHRDRFFQDIPFPYVKQTDICWKLIGPFDHKNDPAKAFE
                     PEEQIRDDYVVEGRRCQWVQARGGTIHINHFFGFEGHLPPAASGTAYGLTYLHSEKEQ
                     DMHFWIGFNGYSRSDRRRGAPNPAQGQWSNVHSAVWVNDQPVGPPVWKQPDAGTSLEI
                     PFVDEDYFYRPPVRIKLRAGWNKILIKAPKAPPAWKWMFTCVPVEWDGRRAVEAKGLV
                     FSTAPQ"
     gene            15638..19840
                     /locus_tag="PLE88_06125"
     CDS             15638..19840
                     /locus_tag="PLE88_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM76076.1"
                     /translation="MFPLNGTAVEPSKQKGWYFQQPRSKMYVQTSRIQPAAANHMSRR
                     RKIEHRSSHHLFDECSTAGNGSGNDSKAIGMMKTEKNSKRFENQKAAAVLCLVLGCCV
                     LVQLTKGNKHDWENESVIGWGKEPARPYGWSFASLKSAAQAYAWTTPKELIDTQAASD
                     FVYPLNGSWHFYWVRHPDLRPADFYKPDYDVSSWAKIPVPSNWELHGYGTPIYVNIRY
                     PHAAAPPAVLGEVPPYFTAFRQPNPVGCYRRTFTVPTEWKNKEVYIHFGGVSSAFYLW
                     INGQKVGYSQDSRLPAEFRITDFLRPGDNTVAAEVYRWSDGSYLEDQDFWRLSGIYRD
                     VFLFAAPSVQVWDYAIRCDLDDRYQDAAVAVTAAIRNLSQTESQHKLRVHLAEPNGKS
                     MPSVLCESDLIPIPPAGQQSVTLKADVRNPRKWTAETPVLYTILLELCDASGQTLEVK
                     ACKYGFREIAIQNSRLYINGVPVLLKGVNRHEHDPDRGQAVEPAGMQRDADLMKQHNI
                     NAVRTSHYPNQPLWYDLCDLYGLYVIDEANVESHGMGYGEASLGHVPSWELAHTDRVA
                     RMVLRDQNHPSVIIWSLGNEAGPGRNFQACRNAVLAIDSTRPIHYERMNEVADIDSVM
                     YPSVEWLKQAGQNDSPKPLLMCEYAHAMGNAVGNLQEYWDVIESSRRLIGGCIWDWVD
                     QGLRKYTGFKRADGTPEWFFAYGGDFGDQPNDGNFCCNGLVGPDRHITSKLREVKKVY
                     QYVKFSLGEVGADTIQVRLANRYCFTPLDQFGGRWTLTEDGVPIAGGTFRPPAAEPGQ
                     SADITLAVRQPRIFAGAHYYLTIVLEQTEKTLYADKGHIVACEQLRVPYQAAEMKPVR
                     MDIAGQLTLHQTADGIVAEGQSFTAAWDRRSGTLSSLVYNGREVLHCGRGPQLNVFRA
                     FTDNDQWFKDDFIRAGLKELICTVRDMQVWQVSKNAVQIHIVTDCIAAGREGCGLMHT
                     ALFTVFGNGWIDVQNTIEPYGPMPLLPKVGVQMFIGSDYTTFTWLGRGPHESYVDRKR
                     SADIGLYQGQVADQYEPYVRPQENGNKTDVRWAALTDSSGRGMMVITGGTYSVSAHHN
                     TALDFDRAGHIHRVIPRSEIVLCIDAKHMGLGGASCGPAPMNQYRFTPESTQMHYSIC
                     PVHTSDTRNMAQQARTVLPVPLPPEIADQKVPNAAGGYSRQITLSTPAGTSIVYRLES
                     TNKASEMRPYAGPFVFDQAGTIFAQSCSEDGLKSMAVRADYPPYYDLLELDKSDWKIL
                     YTDSFQPGEGEAHHVIDGRTETYWHTNWQTNRKPFPHEIHVDFSTVYTLAGFRYLARQ
                     DSTNGRINAYEVSVSEDGQHWTTVKQGNLANTAQWQDILFDTPRTARFFKLTALNEHG
                     KSYYASAAELDIMAIRPANEAP"
     gene            19865..21412
                     /locus_tag="PLE88_06130"
     CDS             19865..21412
                     /locus_tag="PLE88_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM76077.1"
                     /translation="MIKKNRSTNNPRYPMDTAKRPSLSFASAPLFTVAVLLLACTAKG
                     AGDDTIEAVIGTKTHVLPHPRQLLWQQREYNAFVHFSINTFTDREWGDGTEDPSLFNP
                     SELDVNQWVRVFKEAGMKMVILTAKHHDGFCLWPSRYTDHSVKNSPWKNGQGDLLADL
                     SEACRQAGLDFGIYVSPWDRHEPSYGNSSEYNAFYMGQLTELLTQYGPLGEVWFDGAP
                     GLDSPLRKNQVYDFEMYRALVRRLQPDACLFADDGPDCRWVGNEDGTAGETCWSLLQR
                     SNFAPGRADRNKLNTGQIDGTDWVPAECDVSIRPGWFYHAAEDRQVKTVEQLLDIYFK
                     SVGRNGLLLLNVPPDRRGLIHPVDRERLLLFRRALDQIFQTDYAKGAAAAASNVRSGR
                     QVYHPQNLLDSDFDSIWATDNGITKGWVELDLPEETRFNVILIQEYIAQGQRVKAFGI
                     DAWIHDQWQPIAEGTTIGYKRLLRVSPVRTRRVRLRIEDSTDCPVLSRLGLYYAPAVK
                     GNEILSE"
     gene            21480..>22780
                     /locus_tag="PLE88_06135"
     CDS             21480..>22780
                     /locus_tag="PLE88_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOM76078.1"
                     /translation="MDRREFVKFLGFQIGSASALVEAMSNRLPQQRPNILFIFSDDHA
                     VQAISAYGSTINQTPNIDRLAREGIILDRCYCCNSICAPSRAAVLTGKHSHANGVMTN
                     MDRFDGTQPTFPQMLRQAGYQTALIGKWHLKSDPTGFDYWDILPDQGNYYNPDFLTPA
                     GKTRHIGYVTDLITDKSLDWLSAQRDARKPFLLMCQHKAPHRNWMPNLKHLHLYEDAE
                     IPEPPTLFDDYASRNPMLKNNEMEIGRHLMMDSDLKVAGSPLKDALGRSFSNAERARM
                     TPEQRQQWDAAYSPRNAAFEKANLSGKDLLRWKYQRYVKDYLRCIASVDENIGRLLDW
                     LEVSGLARNTLVVYSADQGFYLGEHGWYDKRWMYEESFRMPFIARWPGHIKAGIRVSA
                     LAQNIDFAPTFLEAAGLTVPHEMQGVSLCSLFDGKVPNRWR"
BASE COUNT         5149 a         6336 c         6366 g         4929 t
ORIGIN      
        1 ctgttcggac agattctgca cggcgaatgc ggctacctgc atgacttgcg ggccttgaat
       61 ttatccccca cttattatca gggcatgtgg cgggtgtatc attatatcga tatggacggc
      121 aatctctatc cgacccacgg catcggcccg atggcatggt gcatggatat caaccgcggg
      181 gatacgctgg attatctggt ctgcttcagc agcccctcgg tcggcctgca ggaatacgcc
      241 aaagcccatt accccgacag ccgctgggcc cggcaggaat tcgccaacgg cgatgtcgcc
      301 acctgtctga tcaagaccaa actgggcaaa accatcattt gcaaacacga taccagtctg
      361 ccgcggccct acagccggga ttttctggtg cagggcagca aaggcctcat ccagaagtat
      421 ccccagccgg ttatccatat cgagggacgc tcccccgaac atgcatggga aagcttcgat
      481 gggtatctgc aggagtacga gcatcccgtc tggctgaatc tgcgaaagaa aaccgaaaac
      541 gcccccgaca gcggcttggg gcacggcggg atggactata ttgaggatta ccggctgatt
      601 caggccctcc gtcaggggat tgcccccgat atcgatgtgt acgactccgt gctgtggtcg
      661 gcgattattc cgttaagccg gcagtcactg gccaaggggt cggcgccggt tgcgtttcct
      721 gattttacag gcggggcatg gaaacagaaa cgcccgctgg gtgtggaccg gattctgtaa
      781 gcgagcaggg agaagtgatg gcaggacgac acagcacaaa gacggccaag cccttgaaca
      841 gggttgcctt gagcaaggtg gaccaatacg ccttgacact gtccgattac gactgtctgt
      901 atccgccggc agagaaaatc ccggccattg tggtgccgca tttccccctg ctgggcaaac
      961 tgacggccct gcggtttctc gaatgggtcc agcagcatcc cggcggcgtg gtgtccctgc
     1021 cgaccggcaa gacgcctgag tttttcatca agtgggtccg ccggtttctg cgggattggg
     1081 acaagccggc cgttcaggcc gaattggagc aggccgggct ggatccatcc gtcaggccgg
     1141 cgatgaaaag cctgtatttt gtgcagattg acgaattcta cccgattcat tccgagcaga
     1201 ccaacagctt ttcgtattat gtcaaaaagt actatctcaa gggcttcgga atggacccgg
     1261 ccaaggcgat gctgattgac ccctgccgga tcggcatccc aaactgccgg cagctggatg
     1321 ccgtctggcc tgataaatat gtggacctgt cgctgcgctg ccgcagtccc aaaaacaagc
     1381 tggaacagca gcagaaacag ctgctcgagc ggatcgacca gtggtgcatg gactacgaaa
     1441 gcagaatccg ccagcggggc ggaatcggat tctttttagg cggcatcggc cccgacggcc
     1501 acatcggctt caacatcagc ggctcagacc attattccac cacgcggctg tgtgcgacca
     1561 attatgaaac gcaggcggcc gcggcggggg atctgggcgg catcgagatt gcccgcaagt
     1621 gccatgtcat caccatcggc ttacacacca ttacctccaa tccggactgt acggccatca
     1681 tcatggccgc cggcgaatcc aaagccgccg tcgtggcaaa agccatccag tccgaccccg
     1741 atgtgaagta tccggcaacc gccctgcaga aactgcccaa cgcccgcttt tacctcaccc
     1801 aaggcgcagc caaacttctg gtccagcggc actatcagga tgtgtgcacc gccagccagc
     1861 tgtcggacag tcaggtcgaa cagattctgg ccgaactgtc cgtccagaag cgaaagccca
     1921 tcatccggct gacgccgcac gactaccaaa gcaatccgtt cagcaggcag ctgctcaaaa
     1981 aacgcaagga aaccgtcgcc cagctcaacc agctggccgc cgacaatctg gaacgcaaaa
     2041 tcgccaaagg catggaatcg ctggagaaca cctgctttct gcataccgag ccgcatcacg
     2101 atgatattat gctgggctat tttgcccaaa tcgcacggca tttccgcaaa gccagcaacc
     2161 gccattattt tctgaccttt accagcggct ttacatcggt ctccaatccg ttcatggtcc
     2221 ggcaggccgt caatctccag cgctttgtgc attcggagga attcgcctat ctgtatcggc
     2281 aagggtattt cgacccgacc aatcaaatgg cccgcaaccg cgatgtctgg cagtatctgg
     2341 acggcgtggc cgcttccaat gcggatatga aaaacgaggg ctgtgcccgc cgctttctgc
     2401 ggaatctctt tgaactgtat cccgtcaagt ctctcagcgg catcgataag attctgaccg
     2461 acctgctgga gtatttcagg acccagtatc ccggcgggct cgacccgcac catatccagc
     2521 tgctcaaagg ccggtgccgg gagtgggagg ccgaatgcct gtggggctat tacggctgga
     2581 actgcgatgc ggtatcgcat ttgcggctgg gcttttatac cggcagcatc ttcacccccc
     2641 agccgtcccg ccggcgggat gtcctgccta tcgtccggca tttgcagcgg gttcagcccg
     2701 atgtcatatc cgtggcgctg gacccggaag gcagcggccc cgacacccat tacaaggtgc
     2761 tgcaggccac cgccgaagcg ctgcggatct accagcagca gacccgccgc accgacatcc
     2821 gaatctgggg ctatcgcaat gtctggtacc gctttgacac gtcggaggcc aatgtcttcg
     2881 tgccggtaag tctaaatatg ttttcggtga tgcaccagtc ttttgaaaat gcgtttctca
     2941 gtcagaaaga ggcctcgttt cccagttatg aatacgacgg ccccttctcc gaactggccc
     3001 agcagattca ggtccagcag taccagacat taaaaacctg ccttggacgc gaatggtttt
     3061 accagcatcg aagcccgctg atacgggcta cccggggctt tgtgttcctc aaagaaatgg
     3121 agccggagga attttaccgc tcctgcagaa aactgcgcga atccattgaa gatatttcct
     3181 gaaaggaagc ggatggcttt tcaaccgaca ttgctgattc ttgccgccgg aatgggcagc
     3241 cgctacggcg ggctcaagca gatcgatccg gtcggccccc atggcgaact cattatcgat
     3301 tactccctct gggatgcgct gcgggccgga ttccgaaaga ttgtgtttgt catccgccgt
     3361 tcgtttgagc aggcctttcg ggaggccgtc gaatccaaga tcggcagccg tgctgaaacc
     3421 gcctatgtct gtcaggaact ggactgctgt ctgggcggct tcgaattgcc cgcgggccgg
     3481 caaaagccgt ggggaaccgg acatgcgatt ctgacagcac aggagatgat ccatgagccg
     3541 tttgcggtca tcaacgccga cgattattac ggtcctgcgg cctatcggat catggccgaa
     3601 cagctccaga cgattcagac ccgcccccac gagtacgcca tggtcggcta catcctgcgc
     3661 aagacgctgt cggcctgcgg agccgtcgcc cgcggcatct gccggcacga ttccgacatg
     3721 tacctgaccg ccatcgccga atgcaccggc atccggcccc aaggccgaca ggcagtctat
     3781 gccgatgcac aggggcacga gcatatgctc agcggcgatg agattgtgtc gatgaatctg
     3841 tggggctttg cgcccgatat ttttgccttc cttcagcagc agttttccgc gtttctgagc
     3901 cgctgcggcc gggataacaa aagcgaattc tacatccccg cggcggtgaa cactctcgtc
     3961 caaagcggac agaaaaaagt gaaaatactg acaacaccgg acagttggtt cggcgtcacc
     4021 taccgcggcg ataaagagat ggctcaggcc tgcattcggg aactgatcag ccggcaggtc
     4081 tatccggacc atctctggga acattctgta cagcaaccac acaccctatc aggagaaaca
     4141 aaatgaacag agcagtcttt tgcgcagcgc tgctgacggc attctctcac acgctgcctg
     4201 ctgcaccggc ggtctgcatt ataccgcagc cggtccagct ggagatgcat cagggcagtt
     4261 ttgaaatgac ccccaagacg gtgattgctg ccgacgcccc cctgcggcct ctggttgagc
     4321 aatttcagca gatgctgcag ccggcgctgg ggtatctgcc ggcagccgtc agcagtgcag
     4381 accggaccaa cggcagctgc attctgctcg aatgcctgcc ggaattagac tcccttggcc
     4441 cggaagggta tcggctcctc gtgaagccgg acagggttgt tcttcaggcc ccgcagcagg
     4501 ccggcctctt ctacggcatt cagacgctcc gtcagctgct gccttcggaa atcttcagcc
     4561 ggcagcccgt gcccgacaga cgctggacaa tcccctgcgt cgagatcacc gactatcccc
     4621 gtttcaaatg gcgcggactg catctggatg tctgccggca ttttatgccc aaggagtttg
     4681 tgaaaaaata catcgacctg ctggccgtcc acaagatgaa cacctttcac tggcacctga
     4741 ccgacgatca gggctggcgg attgaaatca agaaatatcc gaaactgacc gcaatcggcg
     4801 cttggcgcaa agaaaccgtc atcggaaaaa acaccgccac ctatgacgga cagccgtacg
     4861 gcggattttt cacccacgag gacattcgcg aggttgtgga atacgccaga cagcggcata
     4921 tcaccgttgt tccggaaatc gaaatgcccg gccactgctt ggcggcgctg gcggcttacc
     4981 cggaattgtc ctgcaccggc gggccgtttg aagtccgcac cagctggggc atcgaggcca
     5041 atgtgttctg cgccggaaat gatcaggtct ttcagtttct cgaagatgtg ctggatgagg
     5101 tgctggcgct gtttcccagc ccctatatcc acattggcgg cgacgaatgc cccaaagagc
     5161 ggtggaagca atgccccaag tgtcaggcac ggatgaaggc cgaaggattg aacgatgagc
     5221 atcagttgca gagctggttt atcaaacgga tggaacggta tctggcatcc cgcggccggc
     5281 gcttaatcgg ctgggatgag attctcgaag gtggtctggc tcccggcgcc gccgtcatga
     5341 gctggcgggg tgaacaaggc ggcatcgcag cagccaaggc cggccacgag gtggtgatgg
     5401 cccccaacag ttatgcctat tttgactact atcaggccga tccgaagacc gaaccactgg
     5461 caatcggagg ctttgtgccg ctggaaaagg tttacggcta caatccggtc ccggatgtcc
     5521 tctccgccga acagcagggc ttcatcttgg gggttcaagg gcaaatctgg accgagtata
     5581 tcgccaatgg ggactatgcc gaatatatgg cttatccccg cgcctgcgcg ctggcggagg
     5641 ttgcctggac gccgccggcc cagaaagagt acgaagcgtt ttatcagcgt ctgcaggagc
     5701 acctcaagcg gctcaaggcc ctgcaggtca acttccggcc gctggatccg ctccgaacga
     5761 ttgcaggcag ctggaaatcc ggccaaacag gccaaacatt cgctccgctg caatggaatg
     5821 tttcaacggt cctgaaagag gccggaacat acgaagttgc atttcaatac accggcgggg
     5881 cgcaccggct ggatattcag tccgttgaac tgctggccgg cgacacaatc gtcgcatcgg
     5941 attatcactt cgggatcacc ggtggcagga cgcaggacaa tgtctatcgg ttaacggtcc
     6001 gccagtatga cccgacggca gcctatgttc tgcgggcggt tgtccgcagc gatggcggta
     6061 ccgattccaa cggctatgtg tatctgacaa aaaaataaga ccgacggagc aacagccaat
     6121 gacggcggat tttgctgaca ttgtaaaagc gtttcgggtc gagggcatcc tcgacagcgt
     6181 gaccccctgc ggttccggcc atgtcaatga cacctatatc gtcgcatgcc gtcagaacgg
     6241 acagcaccgg cgctatacgc tccagcgcat caaccattcc attttcaccc atccgcagca
     6301 gctgatggac aatgtgcttc gggtgacgca gcatattcgg cacaaactgc agcaggagca
     6361 ggccgctgat atcgagcggc gcgtcttgac tgtcatcccg acacacagcg gcggcagctg
     6421 ctgtcaggat tgccatggct gctattggcg gatgtaccag tatattgacg aggcccagtc
     6481 ccgggactat ttaagcagcc ccgatatggc gtttgaggcc gcccgcatgt tcgggcggtt
     6541 tcagcgcctg ctggtggatt tgccggaacc caaactggcc gaaacaatac cggattttca
     6601 taacaccccc aagcggctgg cggatttcat ccgtgccctc gagtccgatt cgcataaccg
     6661 cgccgctgag gcaaaagagg aaatccgctt tgtgctcgac cacgccggga tgtgcgacca
     6721 tctgctccgg caggtccggc agggacttat tccggaacgc atcacgcaca acgacaccaa
     6781 aatcagcaat gtgctttttg acagccggac cggacaaggc atttgcgtta tcgacttgga
     6841 cacggtgatg cccggccttt ccctgtatga cttcggcgat atggtccgaa cagccgcctg
     6901 tccggcagcc gaagatgagg agaatctgtc taaaatccgc ttggagagga ccttctttgc
     6961 cgcgcttgtc cgcggctata tcagccaaac agcggacttc ttgaccccgg ccgaaaagca
     7021 gcatctggtc tttgccggca aactgatcac ctttgaacag atgatccggt ttttgggcga
     7081 ttatttgaat ggagacagct attacaaaat cagccgcccc ggccacaatc tgatacgcgc
     7141 acggacccag atgaaactgg ttcagtccat ccaagaacag gaggaagcgc tgcatgcttg
     7201 tgtcaattcc ctgctggaga cggtccaata gaacgcacaa acagaattcg ttataacagg
     7261 agatggctat gacgctggca ttgattgatt ggctcattat cgggtctttt ctggcaaccg
     7321 tgattgtcat tgggctgatt gtgtcccgca aaggcagccg cgatgcggcg gcgttttttg
     7381 tcggaggccg gagcatgccg tggtggcttc tgggcgtatc gatggtggcc tgcacatttt
     7441 cctgtgatac gccgaatctg gtcaccggca tggtccgcgg cggcggcgtg gcagccaact
     7501 ggggctggtg ggcatttctg attaccggca tggtaaccgt atttatctat gcccagctgt
     7561 ggcggcgttc aaaagtgatg acggatctgg aattctacga actgcgctac agcggcaaaa
     7621 gcgctgcctt tatccgcggt ttccgctccc tgtatctggg gatctttttt aactgtctga
     7681 ttatggggac ggtgacgctg gccgctatca agattggaca ggtcatcttt gacatcccgc
     7741 cgtgggccgc ggttgtgctc gggtcggtag gcgtggttgt ctatgccacc gccggcggca
     7801 ttcaaagctg catctgggcg gactttttcc agtacagcgt ggccatgttc ggggcggtct
     7861 atgcggcggt ggtggcctgt cagcagccgc aggtcggcgg cttaagcggg cttctgtcca
     7921 atccgaatgt cctcggacgg ctcagctgga tgccggattt taccacatgg ctggcatggg
     7981 tccccctgct gctgatgccc gtggcggtcc agtggtggag cgtctggtat ccgggagctg
     8041 aacccggcgg cggcgggtac attgcccagc ggatgctgtc ggccaaagat gaaaaaaatg
     8101 ccatcggggc aaccttgttt ttcaacttta tgcactatgc catgcggccg tggccgtgga
     8161 ttatcgtggc gctggcttct ctggtggtct acccttcgct ggacagcatc aaagccgatt
     8221 tccccggcat tgacccatcc tatctgagag aagatattgc ctatccggtg atgatttcca
     8281 agctgggacc cggcctgctg gggcttgtcg tcgcctcgct gattgcggcc tatatgtcca
     8341 ccatcggcac gcacctgaac tggggctcat cctatgttgt caacgactgg tacaaacggt
     8401 ttatgaggcc gcaggccggt caaaaagaat tggttcttgc cggccgcgtc tgcaccgtcg
     8461 tgctgatgct gattgccgcc ttttttgcct tgacgtttct ggaagatgcc aatcaggcgt
     8521 ttgagatact cctgctgtcg ggggccggaa caggcgggat ttatctgctc cgctggttct
     8581 ggtggcgaat caatgcctgg actgaaattg ccgcgatggt cgccgctacc atcgcggcaa
     8641 tctggattgt tctgggcacc ggcgacgaag gcacgctcaa atggtatgtc gactgggcgg
     8701 cggttcggga ctatgtatgc gtcattgacg acgccgccgt tgtcgattcg gtgacggctc
     8761 agcagaaaca gctgctggcc cagacggaag aagtccgaaa agaactggac caacggattg
     8821 ccgcgtggcg ccgggcgcgg cgcggcgatg cctcggcccg tactgccgct cgcaccgaat
     8881 acgaggccgc cctcgaccgg ctccatcgca tccagcaggc ggcctccatc ccgcttctgg
     8941 tgcaggcggc cgccgatgaa tccctgcagc cgaaactcca gcagttttac catcagcaga
     9001 agctgtggga actgcagcgc gaaaagaaac ggctcagccg aaccggagac ggcgccccga
     9061 gaagcatcga ggacatcacc atctccttcg ggggcgtggt caagaacggc aagatctatc
     9121 tgttccaccc cctcctgaac aatctgaagt tttccgtcca gctgctggtt tgtgtcggtc
     9181 tggttacaat cgtatggctg atcacgacct gcatcaccaa gccggccgac ccgcacacct
     9241 tgcgccggtt ctataagctt tgccatcccg gcgggccggg ctggcggaaa gtggttcagg
     9301 acgccaaggc cgacggcgaa gagatcgact cgaaaaatgc tgtcggcgac tggaaacttc
     9361 ccatgcagct ggtatgcgtc tttttgggct gcgtggcgat ctatgcatcc ctgtttgccg
     9421 taggcagctt catctacggc catcgaatat ggggatttgc tctttcggct gcggccttgg
     9481 cggccgttgt cctgctgttc aaatcgttct caaaaatcgg cgtggaatcc gaatcctgac
     9541 ggtcggggta tgaacaaccg ctacgcagtc aaaagatgtt cgcaggctgt ctccatcgat
     9601 gccgactgga ataagtcctg ctggcagaca gtcgagccgc tgtctgtaaa ggtgccgaat
     9661 tggcagccgg ccggttctca gccgctcccc cagacagagg tgaaagtgca gtatgacgat
     9721 gcgcatctgt atgtcatctt ccgtgtcagc gattattatg taacagcgct gaccacccag
     9781 acacatggtc cggtctataa agacagctgc gtcgagttct tcttttcgcc ggcttgcggc
     9841 aggccggatt cctattttaa cattgaaaca aactgctgcg gtgtgcttct ggcccagcat
     9901 catacaggcc ctcgacaaaa cagccgtttt ctggacattg cagactgccg gcaaatacag
     9961 attgccggca ccgtctccgg tccgattcgg caggaaattg ccaagccgat gacatgggtt
    10021 gtggagtatg ccgtcccgct ggaaatcctt ctgcattata cggccattga caaacctgcc
    10081 ccgggggttt gctggcgggg aaatttttac aagtgtgccg acggctgttc gcgtccgcac
    10141 tggatcagct ggctgccgat tcccagtgat atcccggatt tccaccggcc ggactgcttc
    10201 gggctgctcg aatttatgtg aaccggcggt atatccaatg aaagcaatac agcagcggca
    10261 gagaaatcgg actccgcggg tcgaaagtgt ctccggcagc tgcgggaata aatcgaggat
    10321 gatagaatga aaaacgcagg cttttttatc tttatgcaaa ttctttcagc ctgtctgtgg
    10381 gcggtccagc cggccatgtt ttatacctct tttgaagagt ttgcacccgg cgagtttacc
    10441 acgctggaaa atgcgctggg aacgtggacg gctgcccaag gcaaggcatc tgtaagcagc
    10501 tattacagaa gcggccggca atgccttcat cttgccggcg gggaaaccgc tgttgaactg
    10561 gtgctgcctg cggacaggcc cgagttttcg catctttcgt tctgggccga acggtttacc
    10621 gcacaagacc cgttccaatt ccgcattctg gccggcagcc agtcggggtg gaaggaaatc
    10681 tataacggcg acgccgccat taaaacggga cgcggtttcc tcagctgggt caaggtcgga
    10741 ctgccggagc agaattgttc ggcgctgcgg tttgtctgta cggcccctcc cggcaagggg
    10801 attttgatag atgacttgca actgcaccgg ccggctgcaa tgacactgca aaacgtgacg
    10861 acggcgcagt gggtcggacc ggtcctgctg cgaaagcccc acaatccggt cttggcggtc
    10921 aaagtggtga cagagggcgt tctgaacccc ctcacggtaa ccagcttgac aatcaccccc
    10981 gaaggcacta ccaataccag cgccatcgag tctttccgga ttttttacac aggaactgag
    11041 gaacggtttt caactgacag cgcattcggc tctgctcaaa atccgtccga taccatgttt
    11101 tttgcaggcc ggcaggtcct gcaggaaggc gtcaattact tttgggtatc ctgtacgctc
    11161 aaagcatctg cgaatttatt cgacaaagta gatgcctcct gcattcagat aactgccgat
    11221 ggacagaatc tggttccaaa tccggcatca ggcccgattg ccaaacgcat tggttacaat
    11281 gtccgggatg ccggcgatga cggcgtcagg ggctaccgga ttcccggatt ggctgctact
    11341 ctgcgaggca ccctgatagc agtctatgac attcggcggc acggcatggg cgacctgccg
    11401 ggcgacatcg atgtagggat gagccgaagc accgacggcg gccaaagctg ggaaccgatg
    11461 aaggtcatca tcgatatggg gccgcctcac gaccagaacg gcgtcgggga tcctgccgtg
    11521 ctggtggaca agaccgacaa caccatttgg gtggcggccc tatggagcaa gggcaaccgg
    11581 gcatggagcg gttcagggcc cgggatgagt cccgatgaaa caggccaatt tgtcttggtc
    11641 aacagccggg atgacgggct gacatggtcg gaaccgatag taattacccc ccagatcaaa
    11701 gatccgaagt ggcgtttggt atttcagtgc cccggcaacg gcattacact ccgggacgga
    11761 acgctggtat tccccgccca gtttaaggat gaaaatgatg tgccctattc cacattggtt
    11821 tacagcaaag atcacggtca aacatggaac atcggctccg gcgcaaaatc caacaccaca
    11881 gaggcccaga ttgctgaatt gaatgacggc tccctgatgc tcaatatgcg ggataatcgc
    11941 gggggctggc gctccgtatg cgtaacgcgc gacttgggca ggacttggca ggaacatcca
    12001 accagccgct gtgccttgcc ggaacccgta tgcatggcca gtttcatacg gttttcatcc
    12061 gtccttgacg gggatacgcg caatatcctg ctgttttcca atcccgccac agccggcgga
    12121 cgcaggaata tgacgatcaa ggccagcctg gatgagggca tgacatggcc gcagcagtat
    12181 cacaccgtgt tttatgaacc cccttgtgct ggctattcct gtatgacccg tatcgataaa
    12241 cataccgtcg gcattctgta tgaaggcggc gacagtgcat tattgatctt tgagaagttt
    12301 ccgattgcgg aaattgttcc ggaatagtct cggcccagtc tggttggcaa tcctgcacct
    12361 gacggcaaac ggccgatcga ttgtctacga cagagaaaca tttttttaca gctgcatgga
    12421 gaaacataat ggagtttaag gatcttgcag aacaataccg aacagaattg ttcgacaatg
    12481 tcattccctt ttggctgaaa tattctcccg atccagtcta tgggggctac tttacctgtc
    12541 tggacaggaa aggacaggtc tatgataccg ataagtttat ttggcttcag tgccggcagg
    12601 tctggctgtt ctcgatgctg tataaccgct gccgaaaaga gcccctgtgg ctggacaccg
    12661 caaaacaggg catcgaattt ctcatccgcc atgggatgga cagcgagggt aactggtact
    12721 ttgcactgga ccggaccgga cagccgctgg tggggccgta caacatcttc agcgattttt
    12781 ttgccgctat ggcactgagt caatacggcc ttgcagcgca ggacagccgc tgcacagatc
    12841 tggccttaca aacctatcag aacatcctcc gtcgaaaaga taatcccaaa ggccgctaca
    12901 acaaacagat tgccaaaacc cgtgcgctgc gcgggctggg atttcctatg attatggcaa
    12961 atttggcatt ggaaatggag catgtgctgt cggccgatga gctgaaccgc attctggaca
    13021 tctgcatccg ggaagtgatg gggctgcatg tagataaaaa acgggggctt gtgtttgaaa
    13081 acgtgcttgc cgacggcagt catctggatt gttttgacgg ccggcttctc aatcccggcc
    13141 acggcatcga agctatgtgg tttcttatcg atgtcggctt gcgccggcag gataaaacgc
    13201 tggtcgaaca ggcagccgaa gttatcctga atacgctgac ctacgcgtgg gatcccgcct
    13261 ttgagggcat tttttacttt atggacagcc aaaacaaacc gccccagcag cttgaatggg
    13321 accagaagct ctggtgggtg catcttgaga cgctggtggc tttggccaag gcctatcgcg
    13381 caacacggcg ttcggcgtgc ctcgaatggt tcaaccgcgt gcaccattat gcctggagcc
    13441 gatttgccga tccgcagtac ggcgaatggt ttggctatct gaaccgccgc ggagaagttc
    13501 tgctgcccct taagggcggc aaatggaaag gctgttttca tgttccgcgg gcattgtggc
    13561 tgtgctataa agaatttgag acactgcagg ccgaagtctg aacagcttcg ccggcagcca
    13621 tccattgcag agaaagggcc atccatgaca acagcattca aaatcttttg ctcgttgcag
    13681 acagcggttt tcttcggcgc ctttttgctg ccggcgctgt ccgtttcggc tgcggaggta
    13741 tcgccggttc agcgtgcaaa agcccctgca agtctgattc cgtacccgca gcaggtccag
    13801 tggcattccg gacaatgcat tctaaacacc tgcacaattg ttcatcagcc ctcgacggca
    13861 tcgccgcaaa tgtccgacat cgtaaacagt cttgtccaga ttctccagtc cgccggcagc
    13921 aaggtatcca ccgaaggcaa tgcggcctat ccgatttggc ttgtcctcaa tgagcagcag
    13981 cccaatcagg cggcagaggc ataccatctt ctggttacag ccgacaagac agtcgtgacg
    14041 gcttcgaagc cggcgggttt attttacgga gtccagacca tccggcaatt gctgcagcag
    14101 gaaaacggca ggatttttct gccctgctgc gagatgacag actggccggc ctttccctac
    14161 cgcggcttta tgcatgatgt gggacggaat tatcaagata tcgaattgct gaaacgccat
    14221 ctggacatta tggcgcacta caaactgaat ctgtttcatt ttcacctgac cgacgatccg
    14281 ggctggcgga ttgaatgccg gatgtatccg cagctgaacg aaccgcaaaa ttaccggccc
    14341 acacgccagc cgggcaagta ttatacctat gagcaaatca atgatctcat tgcctacgcc
    14401 cgtcagcgct gcatccagat cgtaccggaa atcgatacgc ccggtcacag cgaatacttt
    14461 aaaaaggcct ttggatttga aatgcaggat gcagagggaa ttgccgtgct gaagaacatt
    14521 ttaaatgaat ttttcgagca ggtaaacacc ccttggtttc atatgggttc cgatgaagta
    14581 cagctgaaga atccggcatt tatgaaggaa atggcggact ttatacggag caaaggcaaa
    14641 accgcactgg tttggcggcc gggccatttg ccggacacac acgtgattac ccagctgtgg
    14701 acaggacagt cccgccctgt tgagggagtg ccttatctgg attcacaagc caactacatc
    14761 aatcatatgg atcctctggt tggtccgatc cggctgtttt tccagcagcc ctgcagaact
    14821 cccgccggca atgacatggc tctgggcggc attctctgcc actggccgga cgtcaatatc
    14881 agcagccaaa tggatattta tactcaaagt cctgtctatc cggcattggt cacttatgcc
    14941 gaacgcatct ggcgcgggct gccggccaac agggaagact gctgggcaaa gctgccgccg
    15001 gccgaagatc ccggctttca ggactttgcc gcctttgaga aagacctcat cgtgcaccgc
    15061 gaccgctttt ttcaggatat tcctttcccc tatgtaaaac agaccgatat atgctggaag
    15121 ctcatcggcc cgtttgacca caagaatgat ccagccaaag catttgagcc ggaagaacag
    15181 atccgcgatg actatgtcgt cgagggccgc cgctgccaat gggtccaagc acgcggcgga
    15241 acaattcaca tcaatcattt tttcggattt gagggacatt tgcccccggc ggcttcgggt
    15301 acggcatacg gattgacata tctgcactcc gaaaaggaac aggatatgca tttctggatt
    15361 ggcttcaacg ggtattcccg gtcggaccga cgccgggggg ctccgaatcc ggcgcagggg
    15421 caatggagca acgttcattc agctgtctgg gtcaatgacc agcccgtcgg cccgcctgtc
    15481 tggaaacagc cggatgcagg cacgagtctg gaaatcccgt tcgtcgatga agattatttt
    15541 taccggccgc cggttcgcat caagctccga gccggctgga acaagatact gattaaagca
    15601 cccaaagctc cgccggcatg gaaatggatg ttcacgtgtg ttcccgttga atgggacggc
    15661 cgtcgagccg tcgaagcaaa agggctggta ttttcaacag ccccgcagta aaatgtatgt
    15721 gcagaccagc aggatacagc ccgctgcagc caatcacatg agccgccggc gaaaaatcga
    15781 acaccgcagc agtcatcatt tgtttgatga atgcagcaca gcaggaaatg gcagtggaaa
    15841 cgattcgaaa gcgattggaa tgatgaagac agaaaaaaac agcaaacggt ttgaaaacca
    15901 aaaagccgcc gcagtgttgt gccttgtctt gggctgctgt gtgctggtcc agctgacaaa
    15961 gggcaataaa catgactggg aaaatgaatc cgtcatcggc tggggcaaag agcctgcacg
    16021 cccctatgga tggtcatttg cctccttgaa atccgctgcc caagcctatg cgtggactac
    16081 gccgaaagaa ctgatcgata cacaagcggc atccgatttt gtctatccat taaacggctc
    16141 atggcatttt tactgggtcc gccatccgga cctgcgtcca gcagattttt acaaaccaga
    16201 ctatgatgtc agcagctggg cgaagattcc ggttccttcc aactgggaac ttcacggcta
    16261 tggaacgccg atctatgtga acatacgcta cccccatgcc gccgcgccgc cggctgtgtt
    16321 aggagaagtg cccccctatt ttaccgcatt tcggcagccc aatcccgtag gatgctaccg
    16381 acggaccttt accgttccta ccgaatggaa aaacaaagag gtttacattc attttggagg
    16441 cgtttcctct gccttttatt tgtggatcaa cgggcagaaa gtcggctaca gtcaggacag
    16501 ccggctgccg gcggaattcc gcattacgga ctttttaaga ccgggggaca ataccgtcgc
    16561 cgcagaagtg tacagatggt cggacggcag ttatttggaa gatcaggatt tttggcggtt
    16621 gagcggcatt tatcgagatg tcttcctgtt tgcagcccca tcggttcagg tatgggatta
    16681 tgccattcgg tgcgaccttg atgaccgcta tcaggatgct gcggttgccg ttacggccgc
    16741 catccgcaat ctgtcccaaa cagagtccca gcacaaactg cgggtgcatc tggcagaacc
    16801 caatggaaaa agcatgccgt ctgtgctgtg tgaatcggat cttatcccca ttccgccggc
    16861 cggccaacag tccgttacct taaaagccga cgtaaggaat ccccgcaagt ggacagcgga
    16921 aacacctgtg ctttatacaa ttcttctgga gctgtgcgac gcgtccggcc agacgctgga
    16981 ggtcaaggcc tgcaagtacg gatttcggga aatcgcgatt cagaacagcc ggctgtacat
    17041 caacggggtt ccagtgcttc tcaaaggcgt aaaccgtcat gaacacgatc cggacagggg
    17101 acaggccgtt gaaccggcag gaatgcagcg ggatgcggac ctgatgaagc agcacaacat
    17161 caatgcggtg cgcacgtcgc attatccgaa ccagccgcta tggtatgatc tgtgcgactt
    17221 gtacggcctc tatgtgattg acgaagccaa cgttgaatcg cacggaatgg ggtatggaga
    17281 ggcctccctt ggccacgttc ccagctggga actggcgcat accgaccgtg tggcgcgcat
    17341 ggtcctcagg gatcaaaacc atcccagcgt tattatctgg tctctgggca atgaagctgg
    17401 tccgggacgg aatttccaag cctgccggaa tgcggttttg gcgattgatt caacgcgtcc
    17461 gattcattac gagcggatga atgaggtagc cgatatcgac agcgtgatgt atccttccgt
    17521 ggaatggctc aaacaggccg gacaaaatga ctcccccaag cccctgctga tgtgtgagta
    17581 tgcccatgcg atgggcaacg cggtcggaaa tctgcaggag tactgggatg tcatcgagtc
    17641 cagccgccgt ttgatcgggg gatgcatctg ggactgggtt gatcagggct tgcggaaata
    17701 taccggcttt aagcgggccg acggaacacc agaatggttt tttgcttacg gcggagattt
    17761 cggcgatcag cccaacgatg ggaatttctg ctgcaacggc ctcgtcgggc ccgatcggca
    17821 catcacatcc aaactgcgtg aagtgaaaaa agtgtatcag tatgtcaagt tctctctggg
    17881 cgaagttggc gctgatacaa tacaggtcag gctggcaaac cggtattgtt ttactcctct
    17941 ggatcagttt ggcggccgct ggacgctgac ggaagacggg gttccgatcg ccggcggtac
    18001 atttcgtcct ccggctgcgg agcccgggca atccgcagac ataacactgg ccgttcggca
    18061 gccccgcatc tttgccggcg cacactatta tttgactatt gtactggagc aaacagaaaa
    18121 aacactgtac gcagacaagg gacacattgt cgcctgcgaa cagttgagag tgccttacca
    18181 agcagccgaa atgaaaccgg ttcgcatgga tatagcagga cagctgacgc ttcatcaaac
    18241 agcagacggc atcgttgcgg agggacaatc cttcactgcc gcctgggacc gccgcagcgg
    18301 gaccttgtct tcactggtgt acaacggccg tgaagtgctg cattgcggac gcggccctca
    18361 attgaatgtc tttcgcgctt ttacggataa tgaccagtgg tttaaagatg atttcatccg
    18421 ggccggattg aaggagctaa tctgcaccgt tcgggacatg caggtctggc aggtctcgaa
    18481 aaatgccgtg cagatacaca ttgtcaccga ctgcatagca gccggcaggg aaggctgcgg
    18541 attgatgcat accgctttgt ttacagtctt cggcaacggc tggatcgatg tgcagaatac
    18601 aatagaaccc tatggaccta tgccgctgct gccgaaggtg ggggtgcaga tgttcatcgg
    18661 ttcggattat acaacattta catggctggg ccgcggaccg catgaaagct atgtggaccg
    18721 caaacgcagc gccgatatcg gtctctatca ggggcaggta gccgatcagt atgaaccgta
    18781 tgtacggccg caggaaaacg gcaataaaac cgatgtccgc tgggcagccc tgacggattc
    18841 ttcgggccgc ggcatgatgg tcattacggg aggaacatac tccgtcagtg cgcatcataa
    18901 cacggctctg gatttcgatc gcgccggcca tatccatcgt gtgatccccc gcagcgagat
    18961 tgtattgtgc attgatgcca agcatatggg actaggcggg gccagctgcg ggcctgctcc
    19021 gatgaatcaa taccgtttca ctccggaatc gacacagatg cactattcga tctgccctgt
    19081 ccataccagc gatacccgca acatggctca gcaggccaga accgtccttc ctgtccccct
    19141 gcctccggaa atagcagatc aaaaagtgcc caatgcagcc ggcggatact cccgtcaaat
    19201 taccctatcc acaccagcag gcacaagtat cgtttaccgg cttgagagca ccaacaaagc
    19261 ctccgaaatg cgtccgtatg ccggcccctt tgtatttgat caggccggaa caatctttgc
    19321 tcaaagctgt tctgaagacg gattaaaaag catggcggtc cgtgctgatt atcccccata
    19381 ttacgatttg ctggagctgg acaagtccga ttggaaaatt ctgtacaccg acagctttca
    19441 gcccggcgaa ggggaagcgc accatgtcat cgatggccgc actgagacct attggcacac
    19501 caattggcag acaaatcgca aaccctttcc gcatgaaatt catgtggatt tttccacggt
    19561 ctatactctg gccggattcc ggtatctggc ccgtcaagat tccaccaacg gccgcatcaa
    19621 tgcctatgag gtgtctgtca gtgaagatgg acagcactgg actacagtca aacaaggcaa
    19681 tttggccaat acagcccaat ggcaggatat attgtttgac acaccccgca ccgcccggtt
    19741 tttcaaactt acagcactca acgaacacgg aaaaagttat tatgcctcag cagcagaatt
    19801 ggatataatg gcaatcaggc cagccaatga ggccccttag gttttatagc cggagatgaa
    19861 cagtatgatc aaaaagaatc gcagcacgaa caatccgagg tatccaatgg acacagcaaa
    19921 acgcccgtct ctttcattcg catcagcccc gttgtttact gtcgctgtac tcctgttggc
    19981 ctgcactgca aaaggggccg gggatgacac gatcgaggcc gtcatcggca caaaaacgca
    20041 tgttcttccg catccccgcc agctgctttg gcagcagcgg gaatacaatg cctttgtgca
    20101 cttttcaatc aataccttca ccgacaggga atggggcgac gggacagaag atccgtccct
    20161 tttcaatccc tctgaactcg atgtgaacca atgggttcgt gtcttcaaag aggcggggat
    20221 gaaaatggtc atcctcacag ccaaacacca cgacggcttc tgtttatggc caagccgcta
    20281 caccgaccat tcggttaaaa acagtccctg gaagaatggt cagggcgacc tgctggctga
    20341 cctatccgag gcctgccggc aggccggcct tgattttggc atctatgttt ccccctggga
    20401 ccggcatgaa cccagctatg ggaactcttc cgaatacaac gccttttata tggggcagct
    20461 gacagaattg ctgacgcaat acggcccctt gggagaggtc tggtttgacg gggcgcccgg
    20521 cttggacagt ccccttcgga aaaatcaggt ttacgacttt gaaatgtacc gtgcattggt
    20581 gcgcaggctc cagcccgatg cctgcctgtt tgccgacgac ggtcccgact gccgctgggt
    20641 cggcaacgaa gacgggacgg ccggagagac ctgctggtcc ttgctgcaaa gaagcaattt
    20701 cgcccccggc cgtgcagacc gaaataaact gaacaccggc cagattgacg gaaccgactg
    20761 ggtgccggcc gaatgcgatg tttctatacg ccccggatgg ttttaccatg ccgccgagga
    20821 tcgtcaggtc aaaacggtgg agcagctgct ggatatctat ttcaagtccg taggacggaa
    20881 tggtcttttg ctgttgaatg tgccccctga caggcgcggc ttgattcatc ctgttgacag
    20941 agagcgtctc ctgctgtttc gccgggctct ggaccaaatc tttcagaccg attatgccaa
    21001 aggggcggcg gcagcagcgt ccaacgtgcg cagcggcaga caagtgtatc atccccagaa
    21061 cctgctggat tcggatttcg actccatctg ggcaacagac aacggcatta ccaagggctg
    21121 ggtcgaactg gacctgccag aggaaacgcg ttttaatgtt atcctgattc aggagtatat
    21181 agcacagggc cagcgtgtga aggcctttgg gatcgatgca tggatccatg atcaatggca
    21241 gccgattgcg gaagggacaa cgattggata caagcggctg cttcgggtct cgcccgtccg
    21301 aacgcggcgg gttcggctgc gcattgagga ttccaccgat tgcccggtac tcagccgcct
    21361 gggcttgtat tatgcccccg cagtaaaggg caatgaaatc ctctcggaat agacagagcc
    21421 ggccaatacc gctattcagc acagcaacgt caacaaataa ccaatggatg caagaaacaa
    21481 tggacagacg tgagtttgtt aaattcttag ggtttcaaat cggctcggcc agtgcgctcg
    21541 ttgaggcgat gtccaaccgc cttccccaac aacggccgaa tattcttttt attttctccg
    21601 atgatcatgc cgttcaggcg atcagcgctt acggttcaac aatcaatcag acgccgaaca
    21661 ttgaccggct ggcccgagaa ggaatcatac tggaccgatg ctactgctgc aattcgatct
    21721 gtgcgcccag ccgagcggct gttctgaccg gcaaacacag ccatgccaac ggcgtaatga
    21781 ccaacatgga ccgtttcgac ggcacacagc ccacatttcc ccagatgctg cgtcaggcag
    21841 gctatcaaac ggctctgatc gggaaatggc acttgaaatc cgacccgact gggtttgatt
    21901 actgggacat cctgccggat cagggcaatt attacaatcc tgacttctta actcccgccg
    21961 gaaaaacccg ccatatcggc tatgtcaccg acctcattac cgataaatca ctggactggc
    22021 tttccgcaca gcgtgatgcc cgcaagccgt tcctgctgat gtgtcagcat aaggcccccc
    22081 accgaaactg gatgcccaat ctgaaacatc tgcatttgta tgaagatgcg gagattccgg
    22141 aaccgcccac gctattcgat gattatgcca gccgcaatcc aatgctgaag aacaatgaga
    22201 tggagatagg ccgtcatttg atgatggaca gcgatctcaa ggttgccggc agtccgctca
    22261 aggatgctct gggccgcagc ttcagcaatg ccgaacgtgc gcgaatgacg cctgagcagc
    22321 ggcagcagtg ggacgccgca tacagccccc gaaatgcggc ttttgaaaaa gccaatctat
    22381 ccggcaaaga ccttctgcgg tggaaatacc agcggtatgt caaggattat ctgcggtgca
    22441 tcgccagcgt ggatgaaaat atcggacgct tgctggactg gcttgaagtg tccggattgg
    22501 ctcgtaatac gctggtggtg tacagtgccg atcagggctt ttacctcggc gaacacggct
    22561 ggtatgacaa acgctggatg tatgaagaat cctttcgcat gccgtttatt gcccgctggc
    22621 ccggccatat aaaagcaggc attcgggttt ccgctctggc acagaatatt gattttgcgc
    22681 caacgtttct ggaagctgcc gggctgacgg tgccccatga aatgcagggt gtcagtctgt
    22741 gttctttgtt tgacggcaaa gtaccgaatc gatggaggaa
//
LOCUS       DAOTBO010000038        22639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1880_length_22639_cov_5.428091, whole genome shotgun sequence.
ACCESSION   DAOTBO010000038 DAOTBO010000000
VERSION     DAOTBO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_22639_cov_5.428091"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLE88_06140"
     CDS             <1..1314
                     /locus_tag="PLE88_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM76079.1"
                     /translation="MLDSAIASNGGTCFVRLKPWAERKDPRLHVASIAQRLQEQLFMI
                     PDALCLALQPPAIMGLGAAGGFEVRVQDRGGAGLQELARLGSDFVYKAMNDPVITRLN
                     NSFTMSVPQLYIDVDRVKAQTLDVSLGTIFNTLQTCLGSLYINDFNLYGRTFRVIAQA
                     EPHLRTSVEDIARLEVRSRSGQMIPIRTLATVRETVGPRDVTHYNMYPATTITGVARA
                     GYSSGQALQRVKELLDEMLPPSMGYEWSGMSLQEIKAGGKTIYLFLLASLFAYLFLAA
                     QYESWSIPLAIVLAVPLGLLGAAGLTWLRGYDNNIYTQMGIVLLIGVVCKTSILLVEF
                     AKQLHEQGYSIFDAAVEAARLRFRPILMTALTTALGMVPLTVAAGAGAAARRAIGTSV
                     FGGMIVATVLGVVMIPTFYVAVQWTKEKARSIEKQIEQKIIHPYQ"
     gene            1422..2741
                     /locus_tag="PLE88_06145"
     CDS             1422..2741
                     /locus_tag="PLE88_06145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM76080.1"
                     /translation="MRIIQLTPGSGDTFYCENCLRDGLLVQTIRRAGQDIVMVPMYLP
                     LSRDTAEAAQTAPLFFGGVNVWLQQKCPLFGKTPRWLDALFDSPLLLKGVRRLAGMTR
                     AADLGPMTVSMLAGPEGRQAKEVQRLVDFFQQPLHKPDICIFSNLLLAGLAGPIKERL
                     QCKLVCWLQDERGFIEALGMPWSRKVWNRISELLPLFDMFISVSRFYAQVMQECLPIP
                     AEKMAVLPPGIDVSLYEQAAVSAQAPTIGFLGRMCWDNGLDILQEAFCRIVQSGSELS
                     KNLRLLICGGRTSADKKYIERIEKGFENAGIRQQVQFMEDFDFQARHSFLKQLTVLCT
                     PSRQTPGYAMNVLEAMACGVPFAAPNIGVYGEWAQMTGGGILYESNTPLELEKTLRQV
                     LQNPDQLRRQGTNARTAVQNCFDIRKNAQVLMQRLHECRDGTKQCSN"
     gene            2729..3406
                     /locus_tag="PLE88_06150"
     CDS             2729..3406
                     /locus_tag="PLE88_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM76081.1"
                     /translation="MLELINVCKKYSSPAGDVAVLNNINLFIEAGQSVSIMGPSGSGK
                     TTLLNLMGGLDRPTEGKVILEQKNLGQLSGRQLARVRNSQIGFVFQLHYLLPQCTVLE
                     NVLLPTLAVHQSTSQREDSYHRAMNLLERTGLRRYADARPGQLSGGQRQRAAVVRALI
                     CRPRILLADEPTGSLDADTAEQISDLLLELNQSESVALVVATHSEKLAAKARRRFRLE
                     KANLTEV"
     gene            3524..6697
                     /locus_tag="PLE88_06155"
     CDS             3524..6697
                     /locus_tag="PLE88_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM76082.1"
                     /translation="MGDSVQFSLRRLGLLRLGSVKYAMAVPGYFFRQELADDLAAATG
                     ISTAPLLALQGVLESADGSVHISRVNVYGIDRRFWKFALTPPADTTIPDGSVWVSQAV
                     ASRLGGQKTNLLIRLPAESIVSQDLIFSLEEAKTAAWPVTIGATVSDETLGRFGLSAR
                     QEAPLNVFVPIGWLAEKARTAGKANLLLAGDGGQKRLGISALNAKLRLLLHPEDLDLQ
                     IGCPAGQNAIELRSSRIFIEEPIARAALASGSGGTGILTYFVNEIRLNDKSVPYSMIS
                     GVGGIPEFDGLRDDEIVIHEWLADQLGADAGDRLNLTFFKVAPTRQLIEQNELFTVRR
                     VVPMMGVYADSSLMPDFPGLAQVENCRDWKPGIPIKLDKIRPQDELYWDRYKGTPKAF
                     VSLAAAQKIWANRFGSLTAVRWNTDAVTEQAIRTELMQRIDAVSLGFLFEDVRSAADT
                     SASGSTDFAGLFAGLSMFLIFSAAVLLALVFVFHTETRAAQVGLLLACGWDWLRIFAL
                     LMAEGIAVALGGCAAGAAASMLYAKGLIAALNTGFWAGAVGSLNLFFHAAFQTVLKGF
                     SVSVLICIFTIQVALYHRIRRPVHQFLTGTFETYRSGTPIRLKLFAAFSGMLLAAGVG
                     LPWGADQESQAGLFFLCGTLLLAGFFFAAAWGLGWLRLSSRSFARSQPLLAVRNIPRR
                     TWRSLAVLITLACGVFLVLSIGANYRHAGMMIQRRDSGAGGFALLAETTLPLTQLPSL
                     PADPQSGLPAIAPNSIVGMRVYQQEEANCLNLNRVLRPTLLGVKPEELSRRGAFAFQK
                     VIESPAAADGWQLLLQTQDDGTIPAIGDYATVFWGLGKKIGQSLNYRDEGGRNIKLKI
                     VGILKDSVLQGRLLISEDHFIRLFPSEDGMRMFLIETDWKNDSQQAARLSRLYRDMGM
                     EAIPAGQKLAAFYEVENTYMAIFLVLGGFGLILGSAGLGLVLVLNILERRAELAMMQA
                     VGFTGEDVCRMLFMEHAVLLAAGVICGLLPALLAVAPAIAARGQDFPGGRIALIIAAL
                     LVSGAVWIRVAVAGAARMNWLEILKNE"
     gene            6722..8113
                     /locus_tag="PLE88_06160"
     CDS             6722..8113
                     /locus_tag="PLE88_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOM76083.1"
                     /translation="MNKHALIAPIITGLILTMAVYHWVNAFRSFQVPPRLAGQDGRPQ
                     RDQTQTQTAQIEGTLQRFDGSASALQASWPQFRGPSRSGIIHSDVPLARQWPAQGLPK
                     LWEIQLGDGYAAPVVHNGCVYLVDYDLQAEADAIRCFSLDDGKEIWRYSYPVKIKRNH
                     GMSRTIPAVTDRYLVAMGPKCHVTCLDAVSGQFRWMIDLVREFGATEPLWYAGQCPLI
                     VDGKAILAPAGPDVLMMAVDCQTGQIVWKCPNPNKWKMTHTSILPMQFEGMDFYVYAA
                     SGGVAAADAAQGTLLWQTDAWFLRTNVPTPVDAGGGKIFLSAGYNKGSLMLQLERQGG
                     KLIPKVLFTRPPEIFGSDQQTPIFYKGYIYGVRPDKQFVCLDTEGNIVWTSGSENRYG
                     LGPYIIANDMIYLLNDDGVLSLIEASSEKFNLLSQTRVLSGHESWGPMALAAGRLLVR
                     DLTTLVCLDVAAK"
     gene            8183..9211
                     /locus_tag="PLE88_06165"
     CDS             8183..9211
                     /locus_tag="PLE88_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76084.1"
                     /translation="MIKQTNKIYSAVIVGLVLAAAAAAGLIAFFLFDASGSQIKPAPE
                     YSYNIEQYAKIDPELILYRQTGDTLQLRFEKCSAIAVDAVRRMYIAGDQKIAVYDAQH
                     SIQREIELKDEPTCLHIDFDGGLIAGLKNHLVFLDTEGQTRNQWPAPAENALLTSIAA
                     DRDNIFAADAVNKLVWRFSRTGEVQNQIGRKDAAANIPGIVVPSPYFDIAAYPDGLLR
                     VVNPGRHWIEAYTADGHRQWYWGRAGVDIEGFSGCCNPVALAVLPDGGFVTCEKGLVR
                     VKVYSAEGQFIGVAAGPEQLGWSEPLRICTTAEECKSKGFDVAVDSDGRIYVLNTVNN
                     TVKIFEKK"
     gene            9208..9459
                     /locus_tag="PLE88_06170"
     CDS             9208..9459
                     /locus_tag="PLE88_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76085.1"
                     /translation="MNNKHTGQTTRRRLALDALRWAMTSGFGLLSLRLTRRESGIQEH
                     PCIDVKGLTGCRNCPVWDACRLPRALSVKQFINNDKQKS"
     gene            9440..10006
                     /locus_tag="PLE88_06175"
     CDS             9440..10006
                     /locus_tag="PLE88_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM76086.1"
                     /translation="MTNRNPDKLYRRTFLREGLWTAVLTALAAAAAALFAKSRRSPAM
                     VWQIDPLKCIACGRCATHCVLNESAVKCVHAYAMCGYCDLCTGYFVPEPKELSTAAEN
                     QLCPTGAIRRTYVEDPYFEYTIDESLCIGCGKCVKGCGAFGNGSLFLQVRHDRCLNCN
                     ECAIAAACPSQAFKRVPVENPYLLKDQL"
     gene            10003..11301
                     /locus_tag="PLE88_06180"
     CDS             10003..11301
                     /locus_tag="PLE88_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM76087.1"
                     /translation="MSRIPSVLWILLWLVVAAPALSVQRFPPPEFENEYVIPSPTTPM
                     PRSEIYQAADVVVLTAALLAASWLVLKKRSRRAIVLLMLASLAYFGFWRKGCICPIGA
                     IGNISLALFNSDFAIPWTTAAFFFLPLVFALFFGRVFCGAVCPLGALQDIVLLRPIQI
                     PLWLETPLRLLAWLYLLMAILYAAAGSAFLICRYDPFVAFFRFSANPAMWVISFAMLA
                     MAVFIPRPYCRFLCPYGVILRQLARFSKLSVTITPDECIHCRLCENACPFGAIDKPTV
                     EWPQTERSKGRRRLLLLMALLPVLTAAGAALGYALHSKMAMSHPVVRLAYLTGLEQKG
                     LLKELPEEIKAFHAAGQSSEVLCRQADAKQRQFAVGGALAGGGMGLIAGGMLIAGSIF
                     WKRTDYQANRGSCLACGRCFNSCPRHRLRLKRNQQPEKNG"
     gene            11311..13062
                     /locus_tag="PLE88_06185"
     CDS             11311..13062
                     /locus_tag="PLE88_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM76088.1"
                     /translation="MEAQRISQRYSSAVFQSAVGVAIVAGVFCLIIAGMMAVQAYHMK
                     TADPARAAELEAMKVQARQYPADEALARSILQLDTQIRRDQFARLYFLQRGMVLLAAA
                     AAVCVGAVLGAGAYRPRTPVLKPQGDLKMLQIRQAVRIRTALTITLIVMGAAGLFWMF
                     STPAADTLSSGGRAEIPDDSSPVESQTASMEEMLAHWPTFRGPEGLGICRFENIPEQW
                     DGPTEKNILWKAAVPLPGHNSPIVWGNHVYLTGATKERQQIFCFDARSGQLLWAGDVS
                     IPPSLQRDEMDIMEETGYAASTAVTDGRRVCAIFAGGDMGCFSADGRRLWEKHLGVPE
                     SAYGYAASLTAFENLVIVQWDVGFEPGGSKLMALDWLTGKTIWETARPVPNSWSSPTA
                     AQVGGQWRLLTAGSPFVIVYDPKTGAEQFRAACIEGDIAVTPITAGGRIFVVEPYNKL
                     VALDAQNVQDQAAAPILWQAEASMPDICSPVTDGHYIWTLTTEGGLNCFSVSDGSKIY
                     SQQLSGNFLSSPTLVGDVLYLLSEKGTMILARTGQDYAEIRRNEIGEKCFASPAFAPG
                     RIYIRTMNHLFCIGKSD"
     gene            13059..15407
                     /locus_tag="PLE88_06190"
     CDS             13059..15407
                     /locus_tag="PLE88_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOM76089.1"
                     /translation="MSEIDLTIVDACIERIGRQPCYALPLLQAVQEAFGYLPEEAIER
                     LSEKTDCTASTLWSVATFYDQFRLKPAGRYAIRVCVGTACHVKGSDQIYEAFRRYLKI
                     AEGEDTDADRLFTVDRVACLGCCTLAPAVQIGEVTYGHVTQETVGAVLTDFLERQKTA
                     SRHPDRRVGHDVPPEGEIRIGLGSCCVARGSAKLLAAIKQVMEETAIRASIKHVGCVG
                     MCYQTPLLEIVSPSGQSTLYVRVEPEDAKPILLKHFRPSGKLRSLANCICDAVDRILT
                     DSVALPIDRYPADVREPDISDFLSRQKRIATEHCGSLSPVDIDEYKSRGGFQAFQKAL
                     TDLSPEDIIEQIHQSGLRGRGGAGYPTHLKWTAVRLAEAEQKFIVCNGDEGDPGAFMD
                     RMLMESYPFRILEGMMIAARAVGASRGYLYIRDEYPLAVERISRAIEQCHAHGYLGEG
                     IVASDFSLELKIYKGAGAFVCGEETALLKSIEGKRPAPHLRPPYPARQGLWGYPTLIN
                     NVETYSLVPWIIRQGAAAFAQLGTPQSRGTKVFALAGKIRRGGLIEVPMGITIRQVVE
                     EIGGGIEGDKKLKAVQIGGPSGGCVPAEMADISIDYESLHAAGAIMGSGGLVVLDETD
                     CMVDIARYFLEFTQRQSCGRCTFCRIGTKRMLEILQRLCSGQGKADDLDKLHQLAVMV
                     QNTSICGLGKTAPNPVLSTLKYFRREYEAHLNGVCPAGKCRGLITYSINDECIGCTIC
                     AQRCPADAIAMKPYEKHQIDQAKCIRCGTCKSVCPADAVEVK"
     gene            15414..16979
                     /locus_tag="PLE88_06195"
     CDS             15414..16979
                     /locus_tag="PLE88_06195"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOM76090.1"
                     /translation="MITLTIDNQTVEVAAGATILEAARKLGIYIPVLCFADGCKPSTS
                     CMVCVVCVEGFKTLVPACGTRAAPNMVVHTQSEEIAKARKAAIELLLSDHVGDCEGPC
                     EMGCPAGMDIPKMIRQIAAEDWQGAIRTVKQDIPLPAILGRICPAPCEKVCRRAQADE
                     PVAICLLKRFVADVDLDSSNPYMPHCTQPTGRKAAVIGAGPAGLSAAYYLKQAGIECD
                     VYDEHAQPGGALRFADIDRMRLPLSVVEREIGQLLSIGVRFIGNIRVGRDVSIDELKS
                     QYDAFLLAVGPKDAAQAAADWGIAVIDDKIQTDRQKYSTSVKGIFAAGGCTGSRNLCI
                     RAVADGKKAAYAIQSYLLGLNGVPVRTYCHRMGVVEKEQLQFFLKHASGAERTQPLTV
                     NSGYLPQQARQEAQRCLHCDCRKADQCALRQRATELAARQKSWESQEDLVARIVKDKN
                     IIFEPGKCIKCGLCVQTAKKEGQKIGLAFEGRGFEMKIAVPLNKSLADGLTQAALKCI
                     EICPTGALAPAAQ"
     gene            17007..19418
                     /locus_tag="PLE88_06200"
     CDS             17007..19418
                     /locus_tag="PLE88_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM76091.1"
                     /translation="MAVRSFCNVLVAIAALLLLGAHSYGNDWPHWRGPDYDGISKETQ
                     WDPSGKDSKILWEAQVGIGFSAVSVANGKAYTIGNINKETDVVYCFDAVTGKELWRHE
                     YPEKLGAKYYEGGTHSTPTIENGKVYTVSKFGKVFCLDADTGAVVWQIELPFKTPEWG
                     FATSALIVGEKLILNVGSAGLALDKATGRIIWKSDNSEPGYATPVPYMHDGIPAVAIF
                     GKDTVMGIQIADGKQLWSAPWKTQYDVNASDPIIVGKECFVTSGYNRGAGLIDFSQAQ
                     PKMVWENKNMRSQLSGPVLIDGYLYGIDDNQLVCLNWKTGQKKWAFADVKKGAVSAAG
                     STLIVIGEKGMLYTIKASPESCQPIASVPVLNGKCWTMPVLANGRLYVRDTGGHLVCV
                     DMQDKNAPLVTPAIVPAENPQWPQWQGPNRDNLSSETGLLKQWPAEGPKMLWAAEGIG
                     EGYSSPTIAEGRIYITGMKDNEGFLTCLDMDGKQLWTANYGPEWRRATPGVRCTPTVD
                     NGCVYVISGTGQVGCFDAQSGQKLWLVDAFGQLEGQYGMWGIAESPLIVEDKVIFIVG
                     GKKAMVAALNKKDGSTVWTVPSNGSRSAYSSPIAFQWGGKTSIAGMTESVLFGLNAAD
                     GTVLWTFPIQDYVSRNQKIHPNTPYFKDGLIFYTSGYDMGAVQLRIAPDAASVEKVWT
                     NPEIDCHHGGFVVLDGYIYTSDWRGNDNGYWLCADWKTGKILWTHKWLNKGSLTFADG
                     LLYCYAEKDGQVGLVKPSPEAFNLISAFPITKGQKEHWAHPVVCGKTLYIRHGDVLMA
                     YDIAA"
     gene            19431..20258
                     /locus_tag="PLE88_06205"
     CDS             19431..20258
                     /locus_tag="PLE88_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOM76092.1"
                     /translation="MTIVLCRCRNAGWIAEDILQSALELLKPAGRRLVCVDDLCALAA
                     EQDPLFQQWAAKENLVVAACFERAVRALFAHAGIREQLVARFVNLRAIQTPQEAKAAV
                     RNAIPEQGCPDALSGEPVDIQTAQSDWVPWFPVIDRQRCRNCMQCLNFCLFGVYGQVD
                     QTVRVLRPKKCKTGCPACARVCPYAAIIFPKYDKSPINGDAVDQQQWEQASAASAESF
                     KHRLSANIYQFLKNRAQNSPDSLSPDLRKLKDQFDIPDSIFNPPPSQSGPPERSENG"
     gene            20328..21935
                     /locus_tag="PLE88_06210"
     CDS             20328..21935
                     /locus_tag="PLE88_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOM76093.1"
                     /translation="MVFILHRYIFRELFRVFLLATVAMTLMLSAGMLVPIISQFGVSP
                     QQMLHLLGYFLPITLTFVLPMSALFAASMIYGRLAADRELDACRASGISLWTLVYPGL
                     TLSILVAAANLGLSFYVAPAFVHRSERSVKANAEQILFRNIQRKGYYALPQSRYKLFA
                     DFADPKNNLLEGVVIVDLAEDRPARLVTAERARVRIDTYSDYNEAAIVAQNSHRLDEV
                     APAFIGNVSITTRFASLLTDKIKFQKLEQLKRIQADKMNYFPICQQALEVRAQLAMKL
                     LAADINAQMQTTSEYYQFEDADRTRVYRLSVGQCQIDPKKPSRIELTGPVRLLQIDAL
                     RNSLTIQYDSPRGVLAFELEGGDLRLEMTLDNPLWQRPGVPSGRSLQKYVNNIRFPEK
                     LAGLIPADQFLSKAGDIVLGQSDLPVAADASLKAACRNLLNRLQRVDNEIYAEIHSRL
                     VLGLGCVALILTGIALGIQFRGGHMLTAFGASAIPAGLLVVFILSGKELTKNPAAPAM
                     TGVTVMWTGLIVLGIVTLISYRKLLRT"
     gene            21952..>22639
                     /locus_tag="PLE88_06215"
     CDS             21952..>22639
                     /locus_tag="PLE88_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOM76094.1"
                     /translation="MMKILDRYIARNFLVGYVIALCVMLGMFLTVDLFVHLDEFAELR
                     SVDVPADAAWGNVVLAIGRFYAIRCCLWYKDMAGMIMVVAAVFSLARMTRSNELIAVM
                     ASGTSLKRILAPILFLSLLLTGLMIVDQEAVIPRFSYELSRKHDELSQQGTFDIWFIG
                     DNNGSLFCSPRFDEKNRVLYEPLIILRQPRADSEDIFRIRGKIQADAAVYDSQRKGWI
                     LTNGRWMQVSE"
BASE COUNT         4958 a         5724 c         6474 g         5483 t
ORIGIN      
        1 atgctggaca gtgcgatagc ctccaacggc gggacctgct ttgtccggct caagccctgg
       61 gccgagcgca aagacccgcg gctgcatgtt gccagcattg cacagcgtct gcaggaacag
      121 ttgtttatga tacccgatgc cctatgtttg gcgctgcagc cgccggcgat tatgggattg
      181 ggcgcagccg ggggttttga agtgcgcgtg caggaccgcg gcggggcagg gctgcaagaa
      241 ctggcgcggc tcggcagcga ctttgtgtat aaggcaatga acgacccggt gattacccga
      301 ctgaacaatt cgtttacgat gtctgttccg cagctgtata ttgatgtgga ccgcgtcaag
      361 gcccagacgc tggatgtgtc ccttggtaca atttttaata cgcttcaaac ctgcttgggc
      421 agtctttaca ttaatgactt caatctgtat ggacggacgt tccgcgtgat tgctcaggcc
      481 gaaccgcacc tgcgtacttc ggttgaggat attgcccggc tggaagttcg aagccgcagc
      541 ggccagatga ttcccatacg cacactggca accgtgcgcg aaacggtagg cccgcgggat
      601 gtaacgcatt acaatatgta tccggccaca acgattacgg gtgttgccag agccggctac
      661 agttccggtc aggcgctgca gcgtgtcaaa gaactgctgg atgaaatgct ccccccctcc
      721 atgggctatg aatggtccgg gatgagtctt caggaaatta aagccggcgg aaaaacaatt
      781 tatctgtttc tgctggcatc gttgtttgct tatctgtttt tggctgccca gtatgaaagc
      841 tggtcgattc cgctggcgat tgtgctggct gttcctctcg gtctgctggg agcggccggt
      901 ttaacttggc tgcggggcta cgacaataat atctataccc agatgggaat tgtcctgctg
      961 ataggtgtcg tctgtaaaac ctccattctt ctggtggaat ttgccaagca gctccacgag
     1021 caggggtatt ccatttttga tgcagccgtc gaggcagcac ggcttcggtt ccggcctatt
     1081 ttaatgaccg ctttgacgac agcgctggga atggttccgc ttacggttgc cgcaggagcc
     1141 ggagcagcgg ctcggcgagc cattggaaca tcggtgttcg gcggaatgat tgtggcgacg
     1201 gtcttgggag tggtgatgat tcccactttc tatgttgctg tccagtggac caaggaaaag
     1261 gcacgcagta tcgaaaagca gattgaacag aagattatcc atccttatca gtaggataac
     1321 agctgccgca tcttcggcaa gatcccggat atgagggatt gcacagacag gccttttcgg
     1381 gtatcctgat gccgtcaatg gatgcttgat gaggatttga gatgcggata atacaattga
     1441 cgccgggctc cggggatacg ttttattgtg aaaattgtct gcgggacggc ctgttggtgc
     1501 agactatccg gcgcgcgggg caggatattg tgatggtgcc gatgtatctg ccgctcagcc
     1561 gcgatacggc cgaagcggcc cagaccgctc ctttattttt cgggggagtg aatgtctggc
     1621 tgcagcagaa atgtccgctt tttggcaaaa caccccgctg gctggatgcc ctgtttgaca
     1681 gtccattgct cttaaagggt gtccgcagac tggccggaat gacacgggct gcagatttag
     1741 ggccgatgac ggtttcaatg ctggccggtc ccgaaggccg gcaggccaaa gaagttcagc
     1801 ggctggtgga tttttttcag caacctctcc ataagccgga tatctgcatc ttttccaatc
     1861 tgcttctggc cggattggcc ggaccgatta aagaacgctt gcaatgcaaa ttggtttgct
     1921 ggcttcagga tgaaagggga tttattgaag cattagggat gccttggagc cgtaaagttt
     1981 ggaatcggat ttccgagctt ctgcctcttt ttgatatgtt tatttcagtc agccgctttt
     2041 atgcccaagt gatgcaggaa tgtttgccta ttcccgccga aaaaatggca gttttgccgc
     2101 cgggtattga tgtgtccctg tatgagcagg ccgctgtatc tgcccaagct ccgaccattg
     2161 gttttctggg ccgaatgtgc tgggataacg gattggatat tctgcaggag gccttttgcc
     2221 gaatcgtcca aagcggcagc gaactatcaa aaaatcttcg gcttctgatt tgcggcggaa
     2281 ggacttcagc ggacaaaaaa tatatcgagc gtatagaaaa gggattcgag aatgccggta
     2341 tcagacagca ggttcagttt atggaagact ttgattttca ggcacggcac agttttctga
     2401 agcagctgac ggtattgtgt accccgagcc gccagacgcc gggatatgcg atgaatgtgc
     2461 tggaagcgat ggcatgcggc gttccgtttg ctgcgccgaa catcggggtg tatggtgagt
     2521 gggctcaaat gaccggcgga ggaattttat atgaatccaa tacacctttg gagctcgaaa
     2581 aaacattgcg tcaggtattg cagaatccag accagctcag acgacagggg accaacgccc
     2641 ggacagctgt gcagaactgt tttgatattc gcaaaaatgc ccaagtactg atgcagcggc
     2701 tgcacgaatg ccgcgacgga accaaacaat gctcgaattg ataaatgtct gcaaaaaata
     2761 ttcctcgcca gcaggggatg tagcggtgct gaacaatatc aatctgttca ttgaagccgg
     2821 acaaagcgtg tctattatgg ggccgtccgg cagcggcaaa acaaccctgc tgaacctgat
     2881 gggcggcctg gataggccca cagaaggtaa agtcatttta gaacagaaaa atctcggaca
     2941 gctctccggc cgccagctgg cccgtgtgcg aaacagccaa atcggctttg tttttcagct
     3001 tcactatctg ctgccccagt gtacggtgct ggaaaatgtt cttctgccca cgctggcggt
     3061 tcatcaaagc acttcccaaa gagaagacag ctaccatcgg gcaatgaatc tgctggaacg
     3121 aacgggtttg cgccggtatg ccgatgcccg cccgggacag ctttccggcg ggcagcggca
     3181 gagagcggct gtggtgcggg cactgatatg ccggccgcgc atcctgctgg cggatgagcc
     3241 gacaggttcg ctggatgccg atacggccga acagatttcg gacctgctgc tggagctcaa
     3301 ccaaagcgaa tcagtggctc tggtcgtggc aacgcattcg gaaaaactgg ctgccaaggc
     3361 ccgccggcgg tttcgattgg aaaaggcaaa tctgacggaa gtttaatttc ggatgacatt
     3421 gttccgtgta ataaaacgcg atttagtttt tttctggagg cgtcatattc caacagcgct
     3481 tctggctgca gtctgctgcg cagtcctgac aggagctata ctggtgggcg attcggttca
     3541 gttcagtctg cggcgccttg gccttctgcg tctcgggtct gtgaaatatg caatggcggt
     3601 tccgggttat ttttttcggc aggagcttgc cgatgacctt gctgctgcaa ccggcatttc
     3661 aacagcaccg ctgctggcgt tgcagggtgt tttggaatca gcggatggtt cggtgcatat
     3721 cagccgtgtg aatgtctatg ggatagaccg ccgcttttgg aaatttgcat tgactccgcc
     3781 ggccgatacg acaatccccg atgggtcggt ctgggtcagt caggctgttg cctcgcgtct
     3841 gggaggacag aaaacaaatt tgctgattcg cctgccggcc gaatcgattg tatcgcagga
     3901 tttgattttt tctctggaag aggccaaaac tgcggcttgg ccggtaacta ttggcgcgac
     3961 ggtcagcgat gaaacattgg gacgttttgg actttctgcc cggcaggagg cgccgctgaa
     4021 tgtctttgtg ccgattggct ggctggcgga aaaagcccgt acggcaggca aagccaatct
     4081 gcttttagcc ggcgacggtg gccagaagcg tcttggtatc tccgctttaa acgccaagct
     4141 taggctcctg cttcatccgg aagatttaga cctgcagatt ggctgtccag caggccagaa
     4201 cgcaatcgag cttcgcagca gccgtatttt cattgaggag ccgattgctc gtgctgcact
     4261 ggcctccggc agcggaggaa ccggaatttt gacatatttt gtcaatgaaa tccgcctgaa
     4321 cgacaaaagt gttccctatt cgatgatttc cggtgtcggc ggaatccccg aatttgatgg
     4381 tttgcgggac gacgaaattg ttattcatga atggctggct gaccagttgg gggcagatgc
     4441 cggtgaccgt cttaacttga cattcttcaa agtcgccccc acccggcaat tgattgagca
     4501 aaacgagctg tttaccgttc ggcgggtagt tcccatgatg ggagtgtatg cagattcttc
     4561 gctgatgcct gattttcccg gactggcaca ggttgaaaac tgccgcgact ggaagcccgg
     4621 catcccgata aaactggaca aaatacgccc ccaagatgaa ctgtattggg acagatacaa
     4681 aggaaccccc aaagcattcg tatcgctggc ggccgcacag aaaatctggg ccaatcgatt
     4741 tgggtccctg accgcggtgc gctggaatac agacgccgtc acggaacagg cgattcgaac
     4801 tgaattaatg cagagaatcg atgccgtatc attgggattt ctattcgagg atgtccggtc
     4861 tgcggctgac accagcgcct ccggttcgac agattttgcc ggtttgtttg ccggattaag
     4921 catgttcctg attttttcgg cagctgttct tctggctctg gtatttgtat ttcataccga
     4981 aacccgcgcc gctcaagtgg ggctgctgct ggcctgcggc tgggattggc tgcgaatatt
     5041 tgcgctgctc atggccgagg gaattgccgt ggcgctgggt ggctgtgcgg cgggtgcagc
     5101 tgcatcgatg ctctatgcca agggattgat tgcggcgctt aacaccggct tctgggcagg
     5161 cgctgtcggc tccctgaatt tattttttca tgcggcattc caaaccgtgc tgaagggttt
     5221 ttctgtcagt gttctgatat gcatattcac catccaagtt gccctgtacc atcgcatccg
     5281 ccgcccggtt caccagtttt tgaccgggac cttcgaaaca tatcgctcgg gtacccctat
     5341 acggctgaag ctctttgccg ctttcagcgg aatgcttctc gccgcgggcg tcggcttgcc
     5401 gtggggggcg gatcaagagt ctcaggcggg attatttttt ttatgcggga cgctgctgct
     5461 ggccggattc ttttttgccg ccgcatgggg cttgggctgg ctgcggctgt ccagccgctc
     5521 ttttgcccgc tcccagccgc ttttagccgt ccgaaacatc ccccgccgca cttggcggtc
     5581 gctggctgtt ttaatcacgc tggcctgcgg cgtctttttg gtgctcagca tcggcgccaa
     5641 ttacagacac gccggtatga tgatacaaag acgcgactca ggggcaggag gatttgccct
     5701 gctggccgag acgacattgc ctctgacgca gcttccttct ctgccggccg atccgcaaag
     5761 cggcctgcct gcgatagcgc ccaacagcat tgtaggaatg cgggtctacc agcaggagga
     5821 agcaaactgt ctgaatctca atcgtgttct gcgcccaacg ctgctggggg taaagcctga
     5881 agaactgtcc cggcggggcg cctttgcatt tcaaaaagtc attgaaagcc ccgctgctgc
     5941 tgatggctgg cagctgttgc tgcaaacgca ggatgacgga acaattcctg caatcggcga
     6001 ttatgcaacc gttttctggg gtttaggcaa aaaaatcggc cagtctctga attatcgaga
     6061 tgaaggtgga agaaatatta agcttaaaat cgtgggaata ttgaaagatt ccgtccttca
     6121 gggacgtttg ctgatttcgg aagaccattt tatccgtctt tttccatccg aggacggaat
     6181 gcggatgttt ctgatagaaa cagactggaa aaatgacagt cagcaggcag cacgattgtc
     6241 acgattgtat cgtgatatgg ggatggaggc gattcctgcc ggccaaaagc tggcggcatt
     6301 ctacgaggtg gaaaatacct atatggcgat ttttcttgtg cttggcggct tcggtttgat
     6361 attgggatcg gctggattgg gattggtact ggtgctcaat atactggagc ggcgtgccga
     6421 actggcgatg atgcaggcag taggattcac cggggaggat gtgtgccgaa tgctttttat
     6481 ggagcatgcg gtcctgctgg cggcaggggt gatctgcgga ttgctgccgg cgctgctggc
     6541 tgtcgcgccg gcgatagcag cccgcgggca ggatttcccc ggcggccgta tcgctttgat
     6601 tattgccgct ttgttggtca gcggcgccgt ctggattcga gtagcggttg ccggtgcggc
     6661 aagaatgaat tggcttgaaa tacttaaaaa tgaataactg tcccagagag ctggcttcaa
     6721 gatgaacaaa catgcactga tagcacctat aataacagga ttgatcctga caatggctgt
     6781 gtaccattgg gtcaatgctt ttaggagctt tcaggttccg ccgcgtctgg ccggtcagga
     6841 cggcagacct cagcgcgatc agacacaaac gcaaaccgcc caaattgaag gcacgctgca
     6901 gcgcttcgac ggcagtgcct ctgctttgca ggcaagctgg ccgcagtttc gcggccccag
     6961 ccgcagcgga attatccatt ccgatgttcc tctggcgcgt caatggcctg ctcagggcct
     7021 ccccaaactc tgggagattc aattgggcga cggatacgcc gctcctgtgg tgcacaacgg
     7081 ttgtgtatat ctggtggatt atgatttgca ggccgaagca gatgctatcc gatgcttttc
     7141 gctggacgac ggcaaagaaa tctggcggta cagctatccg gttaaaataa agcgcaatca
     7201 cgggatgagc cgtactatac ctgcggtaac agaccgttat ctggtggcca tgggacccaa
     7261 atgtcatgtt acctgtttgg atgctgtcag cggtcaattt cgctggatga ttgatttggt
     7321 ccgcgaattt ggcgccacag agccgctttg gtatgccggc caatgtccgc tgattgtgga
     7381 tggcaaagct attctggctc ctgccgggcc ggatgttctg atgatggccg tcgattgtca
     7441 aaccggtcaa attgtttgga aatgtcccaa tccaaacaag tggaaaatga cgcatacctc
     7501 tattttgccg atgcagtttg agggtatgga tttctatgtc tatgccgcca gcgggggagt
     7561 agctgctgcg gatgccgcac aaggaaccct gctgtggcag acagatgcgt ggtttctgcg
     7621 aaccaatgta ccgacccctg tcgatgccgg cggagggaag attttcctgt ctgccggcta
     7681 caataaaggc agtttaatgc tccagctgga acggcagggg ggaaaactta tccccaaagt
     7741 cctgtttacg cgtccgccgg aaattttcgg ctcagaccag cagactccta tcttttataa
     7801 ggggtatatt tacggtgtcc gtccggacaa acaattcgtt tgtttggata cagagggtaa
     7861 tattgtctgg acaagcggtt cagagaaccg gtatggttta gggccgtata tcatcgcaaa
     7921 cgatatgatt tatctattga atgatgatgg agttttgtcg ctgattgaag catccagcga
     7981 gaagtttaat cttttatcgc agacacgagt gctcagcggc cacgagtcct gggggccgat
     8041 ggcattggcg gcagggcgcc tgctggtgcg ggatttgaca acgcttgttt gtctggatgt
     8101 cgcggcaaaa tgacgcggct tgtattgaac agcaaaatcc attatactcc tgtaagtttt
     8161 catagctgta gggttgcttt agttgataaa acagacaaat aaaatctatt ctgcggtcat
     8221 tgttgggctg gttcttgcag ctgcggcagc ggcaggcctg attgcatttt ttttgtttga
     8281 tgcttccggt tcccagataa agcctgcccc ggagtattca tataatatcg aacaatatgc
     8341 aaaaatagat ccggaactga ttttataccg tcagaccggc gacacgctcc agctgcgatt
     8401 tgagaaatgt tctgccattg ctgttgatgc cgtccggcgg atgtacattg ccggcgatca
     8461 aaaaatagcg gtttatgatg cccagcattc catccagagg gaaatcgagc tgaaggatga
     8521 accaacctgc ctccacattg actttgacgg cggcttgata gcgggtttga aaaatcatct
     8581 ggtattttta gatacagagg gtcaaacacg gaaccagtgg ccggcgccgg ctgaaaatgc
     8641 gctcctgacc tctattgcag ccgataggga caacatattt gccgccgatg cagtcaacaa
     8701 gcttgtctgg cggttcagcc gcaccggaga ggttcaaaac cagattggcc gcaaagatgc
     8761 agcagccaat atccccggta ttgttgttcc aagtccctat tttgacatag cggcctatcc
     8821 agacggtctg ctccgtgtag tcaatcccgg ccggcattgg attgaggcct atactgctga
     8881 tggacaccgc caatggtatt ggggcagggc cggtgtggat atcgagggct tcagcggctg
     8941 ctgcaatccg gtggcgctgg ctgtgctgcc ggacggagga tttgtaacct gtgaaaaagg
     9001 attggttcgt gttaaagtgt acagcgccga gggtcagttt atcggcgtgg cggccgggcc
     9061 cgagcagctg ggttggtcag agcccctgcg gatttgcaca acagcggaag aatgcaaatc
     9121 caaaggattt gatgtcgctg ttgattccga cggccgtatt tatgtgctca ataccgtaaa
     9181 taatacagtc aagatatttg aaaagaaatg aataacaaac acacaggaca gactacgcga
     9241 cggcgtctgg ctctggatgc cctgcgatgg gcgatgacat ccgggtttgg actgctgtcg
     9301 ctgcggctga cgcggcggga gtccggcata caggaacatc cctgcatcga tgtcaagggg
     9361 ctgaccggat gccgcaattg tcccgtttgg gatgcctgcc gtcttccgcg ggcgctgtcg
     9421 gttaaacagt ttattaacaa tgacaaacag aaatcctgac aagctgtatc ggcgcacttt
     9481 tttgcgtgaa ggactctgga ctgctgtgct gacagctctg gcagcggcgg cagcggctct
     9541 ttttgccaaa tcacgcagat cccccgcaat ggtctggcag attgatccgt tgaaatgcat
     9601 tgcctgcggc cgctgtgcaa cccactgcgt tctcaatgag tctgctgtca aatgcgtcca
     9661 cgcatatgca atgtgcggct actgcgattt atgcaccggc tattttgttc ccgaacccaa
     9721 agaattgtcc accgccgccg aaaatcagtt atgcccaaca ggggctatcc ggcggactta
     9781 tgtggaagat ccttattttg aatatacgat agatgagtcc ttgtgcatcg gctgcggcaa
     9841 atgcgtcaag ggctgcggcg ctttcggcaa tggttcgctg tttctgcagg tccgccatga
     9901 ccgttgtctg aactgcaacg agtgtgcgat tgcagcagcc tgtccttcgc aggccttcaa
     9961 gcgggtacct gtggaaaatc cctatctatt gaaggatcag ctatgagccg aataccatct
    10021 gtgctttgga ttctgctatg gttggttgtc gctgcgccgg cgctgtcagt gcagcggttt
    10081 ccccctccgg aatttgagaa tgagtatgta atcccctctc cgacaacacc catgccccgc
    10141 tctgaaatat atcaggcggc ggatgttgtt gtattgaccg cggctctgct ggcggcctct
    10201 tggctggttc tcaaaaaacg ctcccgacga gccattgtcc tgctgatgct ggcatctctg
    10261 gcctacttcg gcttctggcg gaagggctgc atctgtccga ttggtgctat cggaaatata
    10321 tcgctggcgc tgttcaattc agactttgcc attccgtgga cgacggcggc atttttcttt
    10381 ctgccgctgg tctttgcgct gtttttcggc cgcgttttct gcggggccgt ctgtccgctg
    10441 ggcgcactgc aggacatcgt gctgctgcgt ccgatacaga ttcctctctg gctggagact
    10501 ccgctgcgcc tgctggcatg gctgtatctg ttgatggcga ttctgtatgc cgccgcaggc
    10561 agcgcgtttc tcatttgccg ctatgaccca tttgtagcgt tctttcgatt cagcgccaat
    10621 ccggcgatgt gggtcatcag ctttgcaatg ctggcgatgg cagtctttat cccccggccg
    10681 tactgccgct ttttatgtcc gtatggcgtc attctgcgtc agttggcgcg gttttcaaaa
    10741 ttatccgtaa cgattacgcc ggacgaatgc atccattgcc gtctatgtga aaatgcctgc
    10801 cctttcggtg ccatcgataa gcccacggtc gagtggccgc agacggaacg ctccaaaggc
    10861 cgccgtcggc tgctgctgct tatggccttg ctgccggttc tgacggccgc aggcgcggcc
    10921 ttgggctatg ccctgcattc gaaaatggca atgagtcatc cggttgtgcg gctggcctat
    10981 ctgacaggcc ttgagcagaa gggtcttctc aaagagctgc cggaggaaat aaaagcattc
    11041 catgccgccg gacagagcag tgaggtactc tgccggcagg ccgatgcaaa acaaagacag
    11101 tttgccgtcg gcggtgcctt ggcgggcggc gggatgggat tgattgccgg cggcatgctg
    11161 attgccggca gtattttttg gaaacggacc gattatcagg ccaaccgcgg cagctgcttg
    11221 gcctgcgggc ggtgttttaa cagctgcccg cgtcatcggc tgcggctgaa gagaaatcag
    11281 caaccggaaa aaaacggatg ataagacagt atggaagccc aacggattag tcaacggtac
    11341 agcagcgcag tttttcagtc ggctgtcggg gtggcgattg tagccggcgt attctgtctg
    11401 attattgccg gcatgatggc tgttcaggca tatcatatga aaacagctga tccagcccgc
    11461 gcagcggaat tggaagcaat gaaagtccaa gcccgtcaat accctgcaga tgaagcgctc
    11521 gcccgctcta ttcttcagct tgatacgcag attcggcgcg accagtttgc acgtctttat
    11581 ttcctccagc ggggaatggt gttgctggca gcggcggcag ccgtgtgtgt gggtgctgtt
    11641 ctgggggccg gtgcctatcg tccccgcact cctgtcttaa agccgcaggg tgatttaaaa
    11701 atgcttcaga ttcggcaagc tgtccgcatt cgcacggctc tgaccattac gctgatagtg
    11761 atgggagccg ctgggctgtt ctggatgttt tcgactcccg ctgctgacac actctcttcc
    11821 ggcgggcgtg ctgaaatccc ggatgattca agccctgtcg aatcccaaac ggcatcaatg
    11881 gaggaaatgc tggcccattg gccgacattt cgcggccctg aaggactggg catctgccgc
    11941 ttcgaaaata ttcccgaaca atgggacggc ccaacagaaa agaatattct ttggaaagcg
    12001 gctgtgccgc tgcccggcca taactccccg atcgtctggg gcaatcatgt ctatttgacc
    12061 ggtgccacaa aagagcgcca gcagattttc tgttttgatg cccgcagcgg ccaattgctg
    12121 tgggcgggcg atgtgtcgat tcctccctcg ctgcagcggg atgaaatgga cattatggaa
    12181 gagaccggct atgcggccag cacagctgta acagacggca ggcgggtgtg tgccattttt
    12241 gccggcgggg atatgggctg tttttcagcg gacggcagac ggttgtggga aaagcatctg
    12301 ggagttccgg aaagtgccta tggctacgcc gcttcgctga cggcgtttga aaatcttgtt
    12361 attgttcaat gggatgtggg ctttgagccc ggcggcagca aactgatggc gctggattgg
    12421 ctgacgggta aaacgatatg ggaaaccgcc cgtcccgtcc ccaattcctg gtcatctccc
    12481 accgccgcac aggtgggcgg gcaatggcgt ctcttaaccg ccggctctcc ctttgtgatt
    12541 gtgtacgatc cgaaaacggg cgccgagcag tttcgggccg cctgcattga aggggatatt
    12601 gcggttactc ctattaccgc cggcggcaga atctttgttg tcgaacccta caataaactg
    12661 gttgcccttg atgcccagaa cgtgcaggat caggcagctg cgcccattct ttggcaggcc
    12721 gaggcgtcca tgcccgatat ctgcagcccg gtcactgacg gccactatat ttggacactg
    12781 acaaccgaag gcggcctgaa ctgctttagt gtttccgacg gcagcaagat atattctcag
    12841 cagctgagcg gaaactttct gtcctcgccg acgctggttg gagatgtgct gtatctgctc
    12901 agcgaaaagg gcacaatgat tctggcccgg actgggcagg actatgccga aatccggcgc
    12961 aatgagatcg gcgagaagtg ctttgccagt ccagcctttg caccgggacg gatttatatt
    13021 cgaacgatga accatttatt ctgtattgga aagtcggatt gagcgagatt gatctcacta
    13081 ttgtagatgc ctgtatcgaa cgcatcggta gacagccttg ttatgcgctg ccgctgctgc
    13141 aggctgttca ggaagcattc ggctatctgc cggaggaagc catcgagcgg ctgtcggaaa
    13201 aaaccgactg caccgcttcg acactttggt cggtggcgac cttctacgac cagttccgtc
    13261 tcaagccggc cggcagatat gccattcggg tctgcgtggg aacggcctgt catgtaaaag
    13321 gcagcgacca gatttatgag gcctttcggc gctatttgaa aatcgccgaa ggcgaggata
    13381 ccgatgcaga ccgtcttttt acagtcgatc gggtggcctg tctgggctgc tgcaccttag
    13441 cgccggcagt gcagattggc gaggttacat acggacatgt aacacaggaa acggtcgggg
    13501 cagttctgac ggactttctc gaacggcaga agacagcaag ccgccatcct gaccgtcgag
    13561 tcggtcacga tgtgcccccg gaaggggaaa tacgcattgg tctgggctcc tgctgcgtgg
    13621 cgcgaggcag cgccaagctg ctggctgcaa taaagcaggt gatggaagaa acggctattc
    13681 gggcaagcat taagcatgtc ggctgtgtgg gaatgtgcta ccaaaccccg cttctggaaa
    13741 tcgtttcccc ttctggccag tcaaccctct atgttcgggt cgaaccggaa gatgccaagc
    13801 ccattctgct caagcatttt cggccttccg gaaaacttcg ctcccttgcc aactgcatct
    13861 gtgatgcggt ggataggatt ttgacggatt ctgttgcttt gccgatagac cgttatccgg
    13921 cggatgtgcg cgaaccggat atttccgatt ttttaagccg tcagaaacga attgcaaccg
    13981 agcattgcgg atccctcagc ccggtggata tcgacgaata caagagccga ggtggcttcc
    14041 aagcgttcca aaaagccctg acagatcttt cacccgaaga cattatcgaa cagattcatc
    14101 aatctggtct tcgcggaaga ggcggggcag gctatccgac ccatttgaaa tggactgccg
    14161 ttcgtctcgc agaggccgag cagaaattta tagtctgcaa cggcgacgaa ggcgaccccg
    14221 gcgcttttat ggaccgtatg cttatggaat cttatccttt ccggattctg gaagggatga
    14281 tgattgccgc tcgcgctgtc ggggcaagcc gcggatattt gtatatccgt gatgaatatc
    14341 cccttgctgt ggagcgcatc agccgggcga ttgaacaatg ccatgcccat ggctatctgg
    14401 gcgaaggaat cgtagcaagc gatttctcgc ttgaactgaa gatttacaaa ggagcagggg
    14461 catttgtctg cggcgaggaa acagccctgt tgaaaagcat cgaaggcaag cggcctgctc
    14521 cgcatctgag gcctccctat ccggcccgac agggattatg gggctatccg accctgatca
    14581 ataacgtaga gacctattcg ctggtgcctt ggattatcag gcagggagcc gcggcgtttg
    14641 cccagctggg cactccgcaa agcaggggta cgaaggtctt tgctttagcc ggcaagatac
    14701 gccgcggcgg cctgattgag gtgccgatgg gtattacgat tcgacaggtg gttgaggaaa
    14761 tcggcggcgg cattgaaggc gataaaaagc tcaaggccgt ccagattgga ggtccctccg
    14821 gcggttgtgt gccggctgaa atggcggata tttctattga ttacgaatcc ctccatgcgg
    14881 ccggggcgat tatgggatcc ggcggtttgg tggtattgga tgaaactgac tgtatggtcg
    14941 atattgcccg gtattttctg gaatttacac agcgccagtc ctgcgggcgg tgcacattct
    15001 gccgtatagg aaccaagcga atgctcgaaa tcctccagcg tctctgcagc gggcagggaa
    15061 aagccgatga tttggataaa cttcaccagc tggccgtgat ggttcagaat accagcattt
    15121 gcggtttggg caaaaccgca cccaatcccg tattgtcaac attgaagtat tttcgccgcg
    15181 aatatgaagc gcatctcaac ggcgtctgtc cggccggaaa atgccgcggt ctgattacct
    15241 attcgattaa tgatgagtgt atcggctgta caatttgtgc ccagcggtgt ccggcagatg
    15301 ccattgcgat gaaaccgtat gagaaacacc aaatcgacca agccaaatgc atccgctgcg
    15361 gcacctgcaa gtcggtctgt ccggcggatg ctgtcgaggt caaataacaa ttgatgatta
    15421 cattaaccat tgacaatcag acagttgagg ttgctgctgg agcgaccatt ctggaagccg
    15481 cccgaaaact tgggatttat atccctgttc tgtgttttgc tgacggctgc aaaccctcaa
    15541 caagctgcat ggtctgtgtt gtgtgcgtgg aaggttttaa aacactggtt ccagcctgcg
    15601 gcactcgcgc tgcccctaat atggttgttc atacgcagtc cgaagagatt gccaaagccc
    15661 gtaaggccgc catcgaactg ctgttgagcg accacgtcgg cgattgcgag ggaccctgcg
    15721 aaatgggctg tccggccggt atggatatcc cgaaaatgat ccgacagatt gctgctgagg
    15781 attggcaggg ggcgattcga actgtcaaac aggatattcc gctgccggcg atactgggtc
    15841 ggatttgccc agccccctgc gaaaaagtct gccgcagggc acaggccgat gaacccgtgg
    15901 caatctgcct tctgaaacgg tttgtggcgg atgtggattt agattcttcc aatccttata
    15961 tgccgcactg cacccaaccg actggccgaa aggccgctgt tatcggtgcc ggtccggccg
    16021 gattgtccgc cgcttattac cttaaacagg ccggcattga atgcgatgtc tatgacgagc
    16081 atgcacagcc cggcggggca cttcgctttg cagatattga cagaatgcgg ctgcctttgt
    16141 ctgtagtcga gcgggaaatc ggccaattgt tgagcatcgg agtccgtttt ataggcaata
    16201 tccgcgttgg cagggacgtt tccatagatg aattgaaaag tcaatatgat gctttcctgc
    16261 tggccgtcgg cccgaaagat gctgcacagg ccgctgctga ttggggtatt gctgttatag
    16321 acgacaaaat acagacagat cgtcagaaat actcgacttc tgtcaaaggt atttttgcgg
    16381 ccggcggctg tacgggttca cgaaatctct gtattcgggc agtggccgac ggcaaaaagg
    16441 ccgcttatgc gatacaatcg tacctgctgg gattaaacgg tgtgcctgta cgaacctatt
    16501 gccatcggat gggagtagtc gaaaaagagc aattacagtt ttttcttaaa catgcctccg
    16561 gtgcagaacg tacacaaccc ctgactgtta atagcggtta tttgccccag caggcccgac
    16621 aagaagccca aaggtgcctg cactgcgatt gccgaaaggc tgaccaatgc gccctgcggc
    16681 agcgggcgac agaattggca gcgcgtcaga aaagctggga aagccaggaa gatttagttg
    16741 cccggattgt aaaggacaaa aatatcatct tcgagccggg caaatgtatc aaatgcggcc
    16801 tgtgtgtgca aacggcaaaa aaggaaggcc aaaagattgg tttggcgttt gagggacgag
    16861 gctttgagat gaaaattgct gtgccgctta ataaatcact ggcagacggt ttgacacagg
    16921 cggcgttgaa atgtattgaa atttgtccga cgggagcatt ggctcctgcg gctcaatgaa
    16981 taaaacttct ttttcgggag aacaaaatgg ctgttcgttc attctgcaac gtattagtag
    17041 caattgcggc acttttactg ttgggggctc attcgtacgg gaatgactgg ccgcattggc
    17101 gggggcctga ttacgacggc atcagcaaag aaacgcaatg ggatccatcc ggtaaagaca
    17161 gcaaaatcct ctgggaggca caggtaggaa tcggtttttc cgctgtgtcc gtggccaacg
    17221 gcaaggccta tacaatcggc aacatcaata aggaaaccga tgttgtctat tgttttgatg
    17281 ccgttaccgg caaggaactc tggcggcatg aatatcctga aaaactgggt gcaaaatact
    17341 atgaaggcgg cacacacagc accccgacca tcgaaaacgg aaaagtctat actgtcagca
    17401 aattcggcaa agtattctgt ctggatgccg ataccggcgc cgttgtctgg caaatcgagc
    17461 ttcccttcaa aacgcccgaa tggggttttg caacctcagc gctgatagtc ggcgaaaagc
    17521 tgattttaaa tgtcggttct gccggtctgg ccttggacaa ggccaccggc cggatcatct
    17581 ggaaaagcga caacagcgag cccggctacg ccacacccgt cccgtatatg cacgatggaa
    17641 tacctgctgt agcgatattc ggcaaagata ccgttatggg tatccagatt gcagatggaa
    17701 aacagctctg gtctgccccg tggaaaaccc agtatgatgt gaacgcctct gatccgatta
    17761 ttgtcggcaa agaatgcttc gttacttccg gatacaatcg gggggccggt cttattgatt
    17821 tttcccaagc ccagcccaaa atggtctggg aaaataaaaa tatgcgctcc cagttgagcg
    17881 ggcctgtttt aatcgatgga tacctgtacg gtattgatga caaccagctg gtttgtctga
    17941 attggaaaac cggacagaag aagtgggcct ttgcagatgt taaaaagggc gcagtcagtg
    18001 ctgccggcag caccctaatt gtcatcggcg aaaagggaat gctctataca atcaaggcct
    18061 cccctgaaag ctgtcagccg attgcgtccg ttccggtgct caacggcaaa tgctggacaa
    18121 tgccggttct ggccaacgga cgcctctatg tccgtgatac aggggggcat ttggtctgtg
    18181 tggatatgca ggacaaaaac gccccgctgg ttacgccggc gattgtccct gcggaaaatc
    18241 cccagtggcc gcagtggcag gggcctaacc gcgataatct cagttccgaa accggccttc
    18301 tcaagcaatg gccggccgaa ggccccaaga tgctgtgggc cgctgaaggc atcggcgagg
    18361 gctactcctc cccgaccatt gccgaaggcc gcatttatat taccggaatg aaagacaacg
    18421 aaggtttttt gacctgtctg gatatggacg gcaaacagct ctggacagcc aattacggcc
    18481 ccgaatggcg ccgtgccaca ccgggcgtac gctgtacgcc cacagtggat aacggctgtg
    18541 tctatgtcat cagcggcacc ggtcaggttg gctgctttga tgcccagtcc ggtcagaagc
    18601 tctggctggt ggatgccttc ggccagctcg aaggacaata tggtatgtgg ggcattgccg
    18661 aatcccccct cattgttgaa gataaggtca ttttcattgt cggcggcaag aaagcgatgg
    18721 tggccgccct gaataaaaaa gacggttcaa cagtctggac agtcccctcc aacggcagcc
    18781 ggagtgccta ttcttctccc atcgccttcc agtggggcgg caaaaccagc atcgccggca
    18841 tgaccgaaag cgttttgttc ggcctcaacg ccgccgatgg aactgttctc tggacattcc
    18901 cgattcagga ctatgtctcc agaaatcaga aaatccatcc caatacaccc tatttcaaag
    18961 atgggctgat tttctatacc agcgggtatg atatgggggc cgttcagctt cgaattgcac
    19021 cggatgccgc cagcgttgaa aaggtctgga ctaatcctga aatcgactgc caccacggcg
    19081 gctttgttgt acttgacgga tatatctata caagcgactg gcgcggcaac gataacggct
    19141 attggctctg cgcggactgg aaaaccggca aaatcctctg gacgcataaa tggctcaaca
    19201 agggcagctt gacctttgcc gacggcttgc tctattgtta tgctgaaaag gacgggcagg
    19261 tcggacttgt caagccgtcg ccggaagcgt ttaatttaat cagtgcgttt ccgattacaa
    19321 aaggccagaa agagcactgg gcacatccgg tcgtctgcgg gaaaacactt tacatccgcc
    19381 acggcgatgt cttgatggcg tatgatattg cggcttaaaa tcgggattgt atgacgattg
    19441 ttctgtgcag gtgcagaaac gccggatgga ttgcagaaga tatccttcaa tcggccttgg
    19501 aactgcttaa gccggccggt cgccgtttgg tctgcgtgga tgacctctgt gcattggccg
    19561 ccgaacaaga tcctttattc cagcagtggg cagccaaaga aaatctggtc gttgcggcct
    19621 gttttgagcg ggctgtcagg gccctgtttg cccatgcggg tatccgcgag cagctggtcg
    19681 cccggtttgt caacctgcgg gcaattcaaa cacctcagga ggcaaaggca gctgtccgaa
    19741 atgctatccc ggagcagggc tgtccggatg ctctttcggg tgagccggtt gatatccaaa
    19801 cagcccagtc ggattgggtg ccgtggtttc cggtcatcga ccgtcagcgc tgccgaaact
    19861 gcatgcagtg cctcaatttc tgcctgtttg gggtgtacgg tcaggttgac cagacggttc
    19921 gggtgctccg gccgaaaaaa tgcaagaccg gctgtcctgc ctgtgcccgt gtctgcccct
    19981 atgcggctat tatctttcct aaatatgaca aaagccccat caacggcgat gcagtcgacc
    20041 agcagcaatg ggagcaggca tcggccgcct ccgctgaatc gttcaaacac cgcctgtcgg
    20101 ccaatattta ccagttcctg aaaaaccgtg cccagaacag tcccgattct ctttctcccg
    20161 acctgcgaaa gctcaaagac cagttcgaca ttcctgacag catctttaat ccgccgcctt
    20221 cccagtcggg tccgcctgaa cgcagcgaaa acggctgaat tttatattgt caatcgctgc
    20281 ggtttgtgcc acaatagcac agacaaattg acaggcaagg aaatccaatg gtttttatac
    20341 tgcacaggta tatttttcgc gaattgtttc gcgtttttct gctggcgact gtcgccatga
    20401 cgctgatgct cagcgccggg atgctggttc cgatcatcag tcagttcgga gtcagtcccc
    20461 agcagatgct ccatctgctg gggtattttc tcccgatcac gctgacattt gtgctgccga
    20521 tgagcgccct ttttgcggcc tccatgattt atggacgatt ggctgctgat cgtgaattgg
    20581 atgcctgtcg ggccagcggt atcagtctgt ggacactggt ttatcccggc ctgaccctgt
    20641 ccattctggt ggcggcggcc aatctggggc tgagcttcta tgtggcaccg gcttttgtcc
    20701 accgcagcga gcgcagcgtc aaggccaacg ccgagcagat attgtttcgc aatattcagc
    20761 ggaaagggta ttatgccctg ccccagagcc gctataagct gtttgccgat tttgcggatc
    20821 cgaagaataa cctgctggaa ggggtggtga ttgtggattt ggccgaagac cggccggccc
    20881 gccttgtgac ggccgaacgg gccagagtcc gcatcgatac atacagcgac tacaacgaag
    20941 cggctattgt cgcccagaat tcccatcggc tggatgaagt ggctccggcg tttatcggca
    21001 acgtgtccat taccacacgg tttgcctcgc tgctgactga caaaatcaaa tttcaaaagc
    21061 tcgagcagct caagcgtatt caggcagata aaatgaacta tttccctatc tgccagcagg
    21121 cgctggaggt tcgggcacag ctggcaatga aattgctggc ggccgatatt aacgcccaaa
    21181 tgcagaccac cagtgagtat tatcaattcg aagatgcaga tcgcacacgt gtctatcgcc
    21241 tgagtgtcgg ccagtgtcaa atcgatccga aaaagcccag ccgcatcgag ctgaccggac
    21301 ctgtccggct gctccaaatt gatgccttgc gaaacagtct gacaattcag tatgacagcc
    21361 cccgaggcgt tttggctttt gaattagaag ggggggattt gcggctggag atgaccctcg
    21421 ataatcccct ttggcagcgt ccgggtgttc catcagggcg ttcactgcaa aagtatgtca
    21481 ataatatccg tttccccgaa aagctggccg gcttaatacc cgccgaccaa tttctgtcga
    21541 aagccggcga tatcgtactg gggcagtcgg atttgccggt ggcggcagat gcttctctca
    21601 aagcggcctg ccgaaatctc ctgaaccggc ttcagcgggt ggacaacgaa atctatgccg
    21661 agattcactc ccggctggtt ttgggattgg ggtgcgtggc cttgatactg acgggtatag
    21721 cccttggcat tcaattccgc gggggccata tgctgactgc cttcggagcc agtgccattc
    21781 ccgccggcct gctggtggtg tttatccttt ccggcaagga gctgacaaaa aatccagccg
    21841 ctcctgcaat gaccggtgtc acggtcatgt ggaccggact gattgtgctg ggcatcgtaa
    21901 ccctgattag ctatcgaaaa ctgctgcgta cataagaagc tgttaagata aatgatgaaa
    21961 atcttagatc gttatattgc gagaaatttt ttggtcggct atgtcatcgc cttgtgcgtg
    22021 atgttaggga tgttcctgac cgttgacctg tttgtccatc tggatgagtt tgccgaactc
    22081 cgttcggtgg acgtgccggc ggatgctgct tggggcaatg tcgttttagc gataggccgc
    22141 ttttatgcca ttcgctgctg tttgtggtat aaagacatgg ccggaatgat tatggttgtg
    22201 gcggccgttt tttcgttggc ccgaatgacc cgcagcaatg agctgatagc tgtgatggct
    22261 tccggtacaa gcttaaaacg cattctggcc ccgattcttt ttctgtcgct gctgctgaca
    22321 gggctgatga ttgttgatca ggaggcagtt attccccggt tttcctatga attgagccgc
    22381 aagcatgatg aattatccca gcagggaaca ttcgatatct ggttcatcgg cgacaacaac
    22441 ggcagtttgt tctgcagccc ccgctttgat gaaaaaaacc gcgttctcta cgaacccctg
    22501 attattctcc gccagccccg tgctgattcc gaagatattt tccgcatccg cggcaaaatt
    22561 caggccgatg ctgctgtgta tgacagccag cgcaaagggt ggattctgac caatggccgc
    22621 tggatgcagg tatcggaaa
//
LOCUS       DAOTBO010000039        22327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_1909_length_22327_cov_6.048761, whole genome shotgun sequence.
ACCESSION   DAOTBO010000039 DAOTBO010000000
VERSION     DAOTBO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 22327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22327
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1909_length_22327_cov_6.048761"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..2956)
                     /gene="ppdK"
                     /locus_tag="PLE88_06220"
     CDS             complement(170..2956)
                     /gene="ppdK"
                     /locus_tag="PLE88_06220"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOM76095.1"
                     /translation="MAQKKAVKTAPNAVKKPVKKAASKAGKMVYFFGGGKAEGNAKMK
                     ELLGGKGANLAEMASLGIPVPPGFTITTEVCTHYYQNKKKYPAGLDKEVAAALAKVEK
                     IMGRKLGDVNNPLLVSVRSGARSSMPGMMETVLNVGLTSKTIPGLIKQSGNERFVYDA
                     YRRLIMMYSDVVMEKAAGIEPKDDEGIRRQLERLMDQMKKAKGYTSDTDLTAEDLKIL
                     CDQFKAKVKDVLGKPFPDDAYDQLWGGIEAVFASWNGKRAVSYRRIEGIPDDWGTAVN
                     VQTMVFGNMGDDCATGVAFSRNPGNGENTFYGEYLVNAQGEDVVAGIRTPAPINKESQ
                     SEHNKHLVTLEKGMPKLYKELVAIKNRLEKHYRDMQDIEFTIEKGRLFMLQCRVGKRN
                     GPAAVRMAVDMVKEKLITKEMAVMRVTPAQLDELLHPIIDPKVEKEQKVLAKGLPAGP
                     GGATGQIVFTANDAVNWAKEGKKVILVREETNPEDVEGMRAAQAILTARGGMTSHAAL
                     VARGWGKCCIVGCGALHVDLAAKTVRVDGTALREGDWITLNGTRGNVYVGQLPMMDAG
                     EENKVLMDFLKICDAVRKLGIRTNADTPEDAARAKAFGAEGIGLFRTEHMFYGKNSEQ
                     PLFCLRKMIMSKTLDERRKALNELFPFVKRDIKATLAAMDGMPVVIRLLDPPLHEFVP
                     QDEARQAELAQSLGISIAELTKRAEDLHETNPMMGHRGVRLGITYPEVTEMQVRAIYE
                     AAAELTKEGKKPYPEIMIPVVCDVNELKNQYAIIDAVYKEVLAKFGLKKLKTLTGTMI
                     EIPRAALVANQIAEYASFFSFGTNDLTQMSFGFSRDDIGGFVPDYVSKGILPGDPFQS
                     IDVIGVGELIKIGIERGRSAKKDLEVGICGEHGGEPASIEFCHKVGMNYVSCSPFRVP
                     IARLAAAQAAVKNK"
     gene            complement(3250..4254)
                     /locus_tag="PLE88_06225"
     CDS             complement(3250..4254)
                     /locus_tag="PLE88_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76096.1"
                     /translation="MALLRTAVVGAGKMGRIHAKVYSKLDNVQLVAVADSNPEKAAAV
                     AEQFDCLAVADPADLIGKVDAVTISAPTAAHYDLASLFIQNKIPVLIEKPLAAAVEQG
                     RDIVKLAKKHNVVVAVGHSERCNPVVQAMKRLHIEPMFIEVTRVSPYPFRSMDIGVVL
                     DVMIHDIDIILSLASSPVKKVDAAGVNVIADHEDICNARIVFENGCIANVTASRLALK
                     TERKVRVFSRQAYLSLDYFRKEGIIIKTDPNVDVVKWIRQHQQAQDFDFSTVNWKDLL
                     HIENLQIEDAEPLKLEQQAFLASILDPSKRPEVTAEEGLAALECAEQILESIRAHRWR
                     "
     gene            complement(4265..5074)
                     /gene="lpxA"
                     /locus_tag="PLE88_06230"
     CDS             complement(4265..5074)
                     /gene="lpxA"
                     /locus_tag="PLE88_06230"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOM76097.1"
                     /translation="MSQQIHPTAVIAPSAQVGSNVVIGPYTVVDEGAVIGDNTILDTH
                     VVIGKNTRLGKHNRVYAGAVIGRPPQVLGFDENSRLGGLIIGDHNVIREYVTIHPSMY
                     PDHNTIVGSGNLLMINVHLGHDCHLEDKIVISNCTQVSGHCKIETGAWLSGMVAVHQF
                     VTIGRWCYAAGYTGINHDVPPFVIVSGHYPLEIRAVNKRGLARAGLSDEQKDAVFDAF
                     KWIWRGDGPILERVKELAQRDGLAEPVRAMVDSLINSSKQRFGRHLELYRE"
     gene            complement(5139..6446)
                     /gene="lpxC"
                     /locus_tag="PLE88_06235"
     CDS             complement(5139..6446)
                     /gene="lpxC"
                     /locus_tag="PLE88_06235"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOM76098.1"
                     /translation="MSQQKTIKSEAKLSGRGLFSGCDVKVVFKPADPDTGVVFVRRDL
                     QPPVYIPVSPQNIAVRQRRTALQNSDAYVETSEHCLAAVSALDIDNLIIEIDAPELPG
                     FEGSSEEFFKTLQKCGIQMQEAEQNPIIITEPIAINEGDKSIYALPSGTGGLSITYDL
                     DYTQHTGIGRQIYSCDLSESHFAQHLARTRTFVLEAEARQMQAMGIGTHLSPKEILVI
                     SSDGPIKNSFRFPDECVRHKVVDLIGDLRLAGRPIIGRIVAYKSGHALNQKLVMQIVE
                     AERRSRKERLLESKPVLDIRRIQRILPHRYPFLLVDKVVEVEPDHWIKAVKNVTFNEQ
                     FFQGHFPGRPVMPGVLIVEAMAQVSGLLFAQTLEHTGKLAVLLTMDSVKIRKSVIPGD
                     QLILTAQADKVRSRAAKCNCTAMVGNEIVAEAQLKFMLVDDEV"
     gene            complement(6424..7449)
                     /gene="lpxD"
                     /locus_tag="PLE88_06240"
     CDS             complement(6424..7449)
                     /gene="lpxD"
                     /locus_tag="PLE88_06240"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOM76099.1"
                     /translation="MLLSELAEKLKAKLTGGGEKTIVGVNTIQDAAPDQVCFLTSAKH
                     AKGLAGSRAAAVITDKPLPECLIPQLVVDNVNKALITAMELFAPKLAGFGGIHPTAVI
                     EPSAQIDKTAAVGPGAYISHGVKIGAHTVIGPGCKIGEKTVIGMHCRLDANVVVYHNC
                     QIGNFCIIQANSTIGSTGFGYAFIDGQHRLIPHNGGVILEDGVEIGANSCVDRAKFGN
                     TIIGAGTKIDNLVQIAHNVRIGRLCLLAGQVGIAGSTVLGNGVAMGGQSGIIDNIILG
                     DGAMVCAKSAVTENVPPGQAVLGMPPQEMHRELKCVAIYQRLPELAKELKELRQRVKQ
                     LESAENH"
     gene            complement(7481..8074)
                     /locus_tag="PLE88_06245"
     CDS             complement(7481..8074)
                     /locus_tag="PLE88_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOM76100.1"
                     /translation="MKNAKLMYTFSAVAVVVSLGIYRQAHLDAAAAAAPAKIGVVNVT
                     QVLENCAKHKQWQEKMQSERTAMENEYKKMKDELDALQANLKLRTPGSEDALRLQSEM
                     LEKKALMDAKDEFYRTKVESEMQRWTESLYQKLLTVVQDVAKTKGLDMVIADEQLDLP
                     APSLRDFMLTVKTKKLLYRSEQYDLTAEVLAALDKAQ"
     gene            complement(8209..10521)
                     /gene="bamA"
                     /locus_tag="PLE88_06250"
     CDS             complement(8209..10521)
                     /gene="bamA"
                     /locus_tag="PLE88_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOM76101.1"
                     /translation="MRQLRSQAAVLLAVCICAGLAGAQDSALSPEGLTIKSLEVAGNV
                     TLTRAEILAVVRSRSGLPFRAETAAEDMRRIGRLEAVERVYYNTRIENGTVVLTYVVE
                     ERNLVRSLALVGNKKLKDAVLLKELGFHTGDYLDVFLVRAGADAMEALYRKKGFPWAK
                     VTVSEEGLLLGQVTYQIEEGPRPKIAHVEYNGNQMLRDGQLSDVVKTKPRKFLFFPVY
                     YTPEQVEQDTQKLLEAYHKKSFLDAQISNKVVFNETKSRAYVTFDVAEGPAYTVNAIR
                     LSGNEFFSSEQLSGNMKLRQNYFYSEAWADFDAKKIKSTYGQQGFIDAQVDAKRTILP
                     EARVDVDYEIKEGSRYRIGEVTVVGNTTLQDRTIRRIMDEEGFTPGQWYNADIARGNG
                     EGELEKIIRQSVVAEAASIVPTGTDPNTRDALVTIREGQTGSIMLGAGIASDSGVIGS
                     ISLTQRNFDITDWPESWSELFTGKAFRGAGQQFRLTASPGTEVSTYSVSFTEPYLFDM
                     PMALNLVGSSFERWRETYDESRLTGRFGLEKRYADDWRRGLSFRLEDVKIENLDWDAP
                     QDVIDVKGSNMLYGTRLYIRKDTTDSRFTPSRGYNFDAGYEQVFGDHTFGILDGTYRW
                     YTTLYEDLAELKTVLETKITAATTVGNAPTFERFYLGGQSSLRGFEYRGVSPRSGPSK
                     EPIGSDWMVVGNAEVAVPLGSQMFSWLFFTDAGLIDDGNVRSSVGTGIQILIPQFFGP
                     VPMRFELAAPITKDQDDDTQVFSFSVGALF"
     gene            complement(10553..11971)
                     /gene="dnaB"
                     /locus_tag="PLE88_06255"
     CDS             complement(10553..11971)
                     /gene="dnaB"
                     /locus_tag="PLE88_06255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOM76102.1"
                     /translation="MTQIKKKQIPRNDSWTDADLQQLLQGRKIPASPEAEAAVLGSML
                     LDRQAIADVIETIGADAFFLTEHRLIFEAIVSLYEKNSEIDLLLLRDELKKRNCLEAV
                     GGVEYLVRVAETVPSAANVAYYTGIVQEKALLRQLAYVCGEILNEACREEGEASEKLD
                     SAEQRVFAVTEKKIRGTAYPVRDLITETFENIEKRKGSHVTGLPTGFEELNYLLAGLQ
                     KGEMIIIAGRPSMGKTSFALNMAEALGADEGIPVAIFSMEMSRHQLVERFLCSRSGID
                     SQLVRKGMLSTDQMAELMRVGGELYEKPIFIDDTPNLNPLVVRAKCRRLKSRYDIQAV
                     FIDYMQLMSLGGYVESRQQEISTISRQLKGLARELDVPVVVLSQLNRGAEGREGHRPR
                     MSDLRESGSIEQDADVIMLLHREAYYHQGDEEWMSANQDKLNLAEVIVAKQRNGPTGT
                     VELIFDGRLTRFKPKSHITEPF"
     gene            complement(11934..12425)
                     /gene="rplI"
                     /locus_tag="PLE88_06260"
     CDS             complement(11934..12425)
                     /gene="rplI"
                     /locus_tag="PLE88_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOM76103.1"
                     /translation="MKVLLVEDVEKLGWLGDVVEVKNGYARNYLLPQGLATIPTEANI
                     KSLEAEKQRRAEIRRLERQQKEQLAARMEGAQVIIAAKANEMGHLFGSVTERDIADAL
                     RQQGFEVQDDMVKMPAGHIKEIGTHEVVLRIASDLRPAVRVVVVSEDQTVDADKKETN
                     TQE"
     gene            complement(12486..12746)
                     /gene="rpsR"
                     /locus_tag="PLE88_06265"
     CDS             complement(12486..12746)
                     /gene="rpsR"
                     /locus_tag="PLE88_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOM76104.1"
                     /translation="MAKSQSNPNTKRRKITATIREQTQCRFCREGKTYIDYKDVETLQ
                     KLLTNRGKIFSRRRSGNCASCQRKVKAAIKRARFMALLPFTA"
     gene            complement(12830..13252)
                     /gene="ssb"
                     /locus_tag="PLE88_06270"
     CDS             complement(12830..13252)
                     /gene="ssb"
                     /locus_tag="PLE88_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOM76105.1"
                     /translation="MANLNKVLLMGNMTRNPQLSYLPSNTPVVEFGLAINRRYKKQDG
                     TMADEPCYVDCRLFGKRAEVVNKYFKKGDPIFVEGRLQYDAWEKDGKKQSKLRVFVEN
                     FEFIGKGGQAAGASPAESSDYEQAPETSYGPAEDDIPF"
     gene            complement(13311..13745)
                     /gene="rpsF"
                     /locus_tag="PLE88_06275"
     CDS             complement(13311..13745)
                     /gene="rpsF"
                     /locus_tag="PLE88_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOM76106.1"
                     /translation="METAVKRLYEGMFLVDSALAAQDWQGILDEVQHILKRADAEIVS
                     LKKWDERRLCYEIQRKSRGTYILAYFNCPTDKVKGIERDVQLSERILRALILRTDGMT
                     QQDIEKPTPAEMQPAQDTSDKPAEQNNAEETSDVAEETAETA"
     gene            complement(13829..14521)
                     /gene="pth"
                     /locus_tag="PLE88_06280"
     CDS             complement(13829..14521)
                     /gene="pth"
                     /locus_tag="PLE88_06280"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOM76107.1"
                     /translation="MTKDWNEVFGCLTGIGSLETKEAPAVKLIAGLGNPGAAYAGTRH
                     NVGFMVLDAIAQQLGAAFSRSRFNALFEEVSAQGYRLLLLKPQQFMNRSGHSVASAAG
                     FYKLQPDKILVVTDDMALPVGRIRLRAKGSAGGHNGLKDVIARLGTDQFARLRVGIGP
                     SGTMDSADYVLSKFAAEEQSIIAQAVHTAAGAALCWAAEGIDAAMNRYNVQTEAGSPQ
                     KNDASGQPPSAM"
     gene            complement(14560..15207)
                     /locus_tag="PLE88_06285"
     CDS             complement(14560..15207)
                     /locus_tag="PLE88_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOM76108.1"
                     /translation="MSETTVLKARIRQKAGSNEAVRLRRQGQLPAVLYGHKKETVSIS
                     LDAHDFLEGLHGGARIFELDTDGRKETVLVKQLQYDYLGKDIVHADLMRVDLSERVKV
                     EVSVELRGTAEGTHQGGMVELILDRLEVECRVSDIPEVIPVNIKHLGLNQAIHAGQIE
                     LPEGMKLITDPEAVVVTCHEAAAAKAEEAAPAEGAEAPTEPEVITERKPTEEAAS"
     gene            complement(15250..16209)
                     /locus_tag="PLE88_06290"
     CDS             complement(15250..16209)
                     /locus_tag="PLE88_06290"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOM76109.1"
                     /translation="MLNNPTIIFSGTSNPELAMAISRYMNIELGKAEITRFPDGEKLI
                     RLESDVRGRDVFIIQPTCAPVDENLVELLIFLDCVRRASAARITAVIPYFGYARQDRK
                     DAGRVPITAKLVANLITSAGASRVLSLDMHAKQLQGFFDIPVDHLTAEPVIVQYLKRK
                     KMTDLTVVAPDVGNMKTASRYATALGAELAIIHKRRLTGQEILCEEIIGSVEGRNIVM
                     VDDMIATAGTITGAAKKVRSLGAREIVVGATHGVFAEPALERLKEAPIDEIIVTDTIP
                     LKESVRKLPHLKVLSVASLIGEAMRRIHSNESISTMFKHYGEL"
     gene            complement(16225..17184)
                     /locus_tag="PLE88_06295"
     CDS             complement(16225..17184)
                     /locus_tag="PLE88_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOM76110.1"
                     /translation="MIDKIAIILAAGQSTRMNTRLPKVLHEVCGRPMLEWVIDACRNA
                     GVQRHIVVVGYGKDQIIGRYRDVSDVEFVEQKEQRGTGHAVMCCRDKLQGFTGQTLVL
                     CGDAPLIRTETLTVLLEKHRQEKAAVTLATAVLPDPAGYGRIIRDSWGNIQGIVEHND
                     CDEKQRAITEVNPGYFCFQTQYLLEVLDKIKPNNAKNEYYLTDALHILLADGHKATAV
                     TAVAAEDAMGVNNRQQLAVVSKIMQQRIQNRLMTAGVTIVDPPNTWIDDRAKIGQDTV
                     IEPFTCISGAVHIGSGCRIGPFAFLQEGAVVADGSVVGVFKQL"
     gene            complement(17213..17284)
                     /locus_tag="PLE88_06300"
     tRNA            complement(17213..17284)
                     /locus_tag="PLE88_06300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17249..17251),aa:Gln,seq:ttg)
     gene            complement(17666..18601)
                     /locus_tag="PLE88_06305"
     CDS             complement(17666..18601)
                     /locus_tag="PLE88_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOM76111.1"
                     /translation="MVASLLLIFGLAGVIVGAHLLVDGASAVARRLRVSDLVIGLTVV
                     AFGTSLPELTVNIFAVVQNQPAVAVGNITGSNIANILLILGLSSLIAPVQVGRGTVWK
                     EIPMCLGGSIVLAFFCAAGAQLSRIEGILLLSLFLVFLAYIVGIARELPGLDNTHEHF
                     KSVSLPAAMVMVAAGLIFLIGGGRAVVQGAVRLAAAAGLSEAFIAATVVAVGTSLPEL
                     AASVAAAVKKKPDIAVGNVVGSNIFNIFLILGISAVLRPLPIAPTLMIGLWIGILSAA
                     TLFFCMFIGKRHQLERWQGGILLILYIVYLILQAQ"
     gene            complement(18655..19122)
                     /locus_tag="PLE88_06310"
     CDS             complement(18655..19122)
                     /locus_tag="PLE88_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOM76112.1"
                     /translation="MFEHYSEHARSVMEHARQEAQRYGHDHLGTEHILLGLLEVKEGC
                     AAYVLKHRSVNLSQAKEQVRKLVLPGSISPEGDYDRLPCTEHAQRVLQDAVNEARMLK
                     QSTIGTEHLLLGLLYEKECIATEVLRNLGLKLDQVRQDVMDFLNSPACISRKE"
     gene            19499..20113
                     /locus_tag="PLE88_06315"
     CDS             19499..20113
                     /locus_tag="PLE88_06315"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOM76113.1"
                     /translation="MKYRKIPDETIRRMPMYLRAFSMLKTEGLETISSRQLAQRLHLN
                     PPQIRKDLSYFGTFGTRGIGYPVAATADQIRDILKLNTPQKAALVGAGRLGSAVAAYP
                     GFGMFGFEIAAVFDNAADKIGQKIGGITVEDAEKISTIRQRGIYLAILAVPAYAAQSV
                     TDKLVKAGIRGILNLSPCYLDVPKRIKVVTIDIATALGMLPYYM"
     gene            20195..21235
                     /locus_tag="PLE88_06320"
     CDS             20195..21235
                     /locus_tag="PLE88_06320"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOM76114.1"
                     /translation="MSVSTVTKEQFKSFVAGLIGSEQMVIGVQAKQDKFAFGPLLRAE
                     DLRLDYDVTILSPRKYLLPPREVLLNYQVCGEAQSIWDCRPMILIGVHPYDMIAINQM
                     DELFRQGQYDAHYMNRRNNITIIACDVATPSMNVFASSMDTATVESGFDILLTDIGSG
                     YVVQADTDKGQALVKKMAGIKPATAEDLAKRQAIQDKNRKTLNKHPLECKPSFIPKLL
                     EKAYDHPIWEEKARLCYSCGSCNQVCPTCYCFDVQDEVNWDLKTGIRYRAWDGCLLEN
                     FATVAGNHNFRKDRSARFRHRLYRKGKYVPAKIGGQIACVGCGRCITACTAKIANPVE
                     VYNTLLEKTDIG"
     gene            21274..22110
                     /locus_tag="PLE88_06325"
     CDS             21274..22110
                     /locus_tag="PLE88_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOM76115.1"
                     /translation="MCNCCNGKNDIYRPELWTITKHRMLNGTEMFMHLEQDSGRGFDY
                     MPGQFVEVSLAGIGEAPISISSSPTQKGLELVVRNVGGLTRSIHKLQTGDKLGIRGPY
                     GSTYPVEQTKGKDLVFICGGIGLVPQRSFIRYALDHRQDYRQITVLIGTKCYDMRLFR
                     EEIALWQERKDMVVMETVDEAHDCWDGNVGVVTTLIPKIESDLPSSAVLVCGPPIMYK
                     FVLMALADAEVPEENIYVNLERRMKCGVGKCGHCQINDKYVCQDGPVFRWSDLALVPE
                     AI"
     gene            22113..>22327
                     /locus_tag="PLE88_06330"
     CDS             22113..>22327
                     /locus_tag="PLE88_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76116.1"
                     /translation="MTKPRVAIFDFACCEGCQLQIVNLEEEILNLIELVDVVEWREAM
                     SEQSHEYDIAIIEGSITREEDAKRLEV"
BASE COUNT         5314 a         5950 c         5486 g         5577 t
ORIGIN      
        1 ataggcaagg aataattttc agttaactga tttgcatcga tgaaaagcaa actcaattca
       61 accaacgata gcaatagatg ttttagatat ggattgtcgt tagtatggaa ttaatagaaa
      121 atcacccagc ccctcggcag gggctgggtg aagattgaaa gcgaaagtgt tatttatttt
      181 taaccgcagc ctgtgcggcg gccagacgag caatcggtac acggaatgga gaacagctga
      241 catagttcat acccaccttg tggcagaact caatgctagc cggctcaccg ccatgttcgc
      301 cgcagatacc aacttcaagg tcttttttgg ctgaacggcc gcgctcaatt ccgattttaa
      361 tcaattcgcc gacaccaatt acatcgatgc tctggaacgg atctccgggc agaatgcctt
      421 tggagacata atcgggcaca aaaccgccga tgtcgtcacg gctgaagccg aagctcatct
      481 gcgtcaggtc gttcgttcca aagctgaaga aggaggcata ttcggctatc tgattggcta
      541 ccagcgcagc ccgcgggatc tcgatcattg ttccggtaag ggtcttcagt ttcttgagac
      601 caaacttggc cagcacttct ttatagactg cgtcgataat cgcatactgg tttttcagct
      661 cgttgacatc gcagactact ggaatcataa tttccggata gggcttcttg ccttctttgg
      721 tcaattcagc agccgcttca taaattgccc gcacctgcat ctcagttact tccgggtatg
      781 taatgcccag acgaacgccg cgatgtccca tcatcggatt ggtttcgtgg agatcctctg
      841 cacgcttggt cagttcggca atgctgatgc ccaaggactg tgccagctcg gcctgtcggg
      901 cctcatcctg cggcacaaac tcatgcagcg ggggatccag caggcgaata accacgggca
      961 ttccatccat agcggccagc gtggctttga tgtcgcgctt gacgaagggg aacagttcgt
     1021 tgagggcttt tcggcgctcg tcgagggtct tggacataat catcttacgc aggcaaaaca
     1081 gcggctgctc gctgtttttg ccgtagaaca tatgctcggt acggaagagg ccgatgcctt
     1141 ctgcgccgaa tgctttggca cgggcggcat cttcaggcgt atcagcgttg gtgcggatgc
     1201 ccagcttgcg tacggcatcg cagattttca agaaatccat cagaaccttg ttttcctcgc
     1261 cggcgtccat catcggcagc tgaccgacat agacattgcc ccgtgtgcca ttgagggtaa
     1321 tccagtcgcc ttccctgagg gcagtgccat caacacggac cgtcttagca gccaaatcga
     1381 catggagggc accgcaaccg acgatgcagc atttgcccca gccgcgagca accagtgctg
     1441 catggcttgt catgccgccg cgggcagtaa gaatggcctg agcggctctc attccctcga
     1501 catcttcggg gttggtctct tcgcggacca gaatgacctt cttgccttcc ttggcccaat
     1561 taacggcatc gttggcagta aagacaatct gcccggtggc accgccggga ccagccggaa
     1621 gacctttggc cagaaccttc tgctcctttt ccaccttggg atcgataatc gggtgaagca
     1681 attcatccag ctgggcagga gtaacccgca ttacagccat ttccttggtg atgagctttt
     1741 ctttgaccat atctacggcc attcgcaccg ccgcaggacc attgcgcttg ccgacacggc
     1801 actggagcat aaacagacgt cccttttcga tggtaaactc aatatcctgc atatcgcggt
     1861 agtgcttttc cagccgattc ttaatggcta ccagctcttt gtaaagcttg ggcattccct
     1921 tttcgagggt taccagatgc ttgttgtgtt cggattgcga ttctttgttg atgggggcag
     1981 gggtacggat accagccacc acatcttcac cctgagcatt gaccagatac tcgccataga
     2041 aggtattttc accgttgccc ggattgcggg agaaggccac gcctgtggcg caatcgtcac
     2101 ccatattgcc aaagaccatg gtctggacat taacagcggt accccagtcg tccggaatgc
     2161 cttcaatgcg gcggtagctg acagcccgct tgccgttcca tgaagcgaac acagcttcaa
     2221 taccgcccca caactggtca taggcatcat cagggaaggg cttgccgaga acatccttaa
     2281 ccttagcttt gaactggtcg caaagtatct tgagatcttc tgccgttaaa tcggtatcgc
     2341 tggtataacc cttggccttt ttcatctggt ccatcagacg ttcaagctga cggcggatgc
     2401 cctcgtcgtc tttgggttcg atgccggctg ccttttccat gacgacatcg gaatacatca
     2461 taatcaaacg gcggtaggca tcatagacga accgctcatt gccggactgc ttaatcaggc
     2521 cgggtatggt tttgctggtc agaccgacat tcagaacggt ttccatcatt ccgggcatcg
     2581 atgaacgagc accggaacgg acggacacaa gcaggggatt atttacatcg cccagcttgc
     2641 ggcccataat cttttcaacc ttggccagag cggcggcaac ttctttatcc aggccggcag
     2701 ggtatttctt tttgttctga tagtaatggg tgcagacctc tgtggtgatg gtgaatccgg
     2761 gtgggaccgg gatgcccagc gaggccattt cggccaaatt ggcgcccttg ccgcccaaga
     2821 gttccttcat tttggcgttt ccttcagctt ttccgccgcc gaagaaatat accattttgc
     2881 ccgctttgga ggcggctttt ttcacgggct tcttaactgc attcggggca gtttttacgg
     2941 cttttttttg tgccattgtg aaaatctccc ttatcttaaa cggttaaaat ttcgcgtatt
     3001 ctggcgtttt tgctgtccta cacagaccgc acgatataaa tcagggtgtc gttcaataaa
     3061 aggatgtgaa atatagccgc aaaacggctc tctgtcaaca atgaaaacaa gataaggaac
     3121 agcttttaga gtgccgaaaa tgctgttttc gaactacata atatgatttt cgaagattta
     3181 tatatctccc cgtcattgtt tttgggcttt accacacaat cttttttagg ttttttaaat
     3241 taaatcacat tatcgccacc ggtgggcacg gatagattcg aggatttgtt cggcgcattc
     3301 aagggcagcc agcccttctt cggcggtaac ttcggggcgt ttggacggat cgaggatgga
     3361 ggccagaaag gcctgctgct cgagtttaag aggttctgca tcttcaatct gcaggttttc
     3421 gatatgcagc aaatctttcc agttgaccgt agaaaagtca aaatcctgcg cctgctggtg
     3481 ctggcgaatc cacttgacga catcgacgtt ggggtcggtc ttgatgatga tgccctcttt
     3541 gcggaaataa tccagcgaca gataggcctg ccgactgaag acacggactt tgcgttcggt
     3601 tttgagtgcc agccgggagg cggtaacatt ggcgatgcag ccgttttcga agacgatgcg
     3661 ggcattgcaa atatcttcgt ggtcggcaat aacattgact ccggcggcat cgactttttt
     3721 gacggggctg ctggccaaag aaagaatgat atcgatgtcg tgaatcatta catccagcac
     3781 cacgccgata tccatcgaac ggaaaggata ggggctgacg cgggttactt caatgaacat
     3841 cggctcgata tgcagccgtt tcatcgcctg cacgacggga ttgcagcgct ctgaatggcc
     3901 gacggccaca acaacattgt gctttttcgc cagtttcaca atgtcgcggc cctgctcaac
     3961 tgcagcagcc aggggttttt caatcagtac cggtatttta ttttgaatga acagggatgc
     4021 caaatcataa tgcgccgccg tgggggcgga aatggtaacg gcatctactt tgccgatcag
     4081 gtcggcagga tcggcgacag ccagacaatc gaactgttcg gcaacagcag cggccttttc
     4141 gggattgctg tcggccacgg cgacaagctg gacattgtcc aacttggaat agactttggc
     4201 gtgaatccgg cccatcttgc cggcacccac gacggcagta cgcaacagag ccattggtaa
     4261 tgtcctattc cctatacagt tccagatgcc ggccgaaacg ctgcttgctg ctgtttatca
     4321 gcgaatctac cattgcgcgg acaggttcag ccagtccatc ccgctgggcc aattccttga
     4381 cacgttcaag aataggaccg tcgccgcgcc aaatccattt gaacgcatca aagacagcgt
     4441 ccttctgctc gtcagacagg ccggcacgtg ctaaaccgcg cttattgacg gcacgaattt
     4501 ccagcggata atggccgcta acgataacaa atgggggaac atcatggtta atgccggtat
     4561 agccggcggc atagcaccag cggccgatgg tgacaaactg atggacggca accataccgc
     4621 tcagccaagc gccggtttcg attttgcagt ggcccgagac ctgcgtgcag ttgctgatga
     4681 caattttgtc ttcaaggtgg caatcgtggc ccagatggac atttatcatc agaagattgc
     4741 cgctgccgac aatggtatta tggtcggggt acatactggg atgaattgtt acatattccc
     4801 gaatgacatt atggtcgccg ataatcagcc cgcccagccg gctgttttca tcaaaaccca
     4861 gcacctgcgg agggcggcca ataacagccc ctgcataaac acggttatgc ttgccgagtc
     4921 gggtattttt gccgatgacc acatgggtat cgagaatggt attgtcgccg ataaccgccc
     4981 cctcgtctac gacagtatag gggccgatca ccacatttga ccctacctgt gcactgggtg
     5041 caatcacagc ggtaggatga atttgctgac tcatactttt tttttagaac ctccaaaata
     5101 taaattggtt attatctcac agtcgcaaat actgctgttt atacttcatc gtccaccagc
     5161 ataaatttca gctgggcttc tgccacaatt tcattgccga ccatagcggt acagttgcat
     5221 ttggctgcgc ggcttcggac tttgtctgcc tgcgctgtca agataagctg atcgcctggg
     5281 ataacggatt ttcgaatttt aacgctgtcc atcgtcagca ggacagcgag ctttccggta
     5341 tgctcaaggg tctgggcaaa cagaaggccg gatacctgag ccattgcctc gacaatcaga
     5401 acgccgggca tcacgggtcg gccgggaaag tgtccttgaa aaaactgctc attaaaggtc
     5461 acatttttga cagcttttat ccagtggtct ggctcgactt caacaacctt atcgaccagc
     5521 aggaatggat agcgatgagg cagaatgcgc tgaatgcggc ggatatccag cacgggtttg
     5581 ctttccagga gacgttcctt tcggcttcgg cgttcggctt cgactatctg catcaccagt
     5641 ttctggttaa gggcatggcc tgatttatag gcgacaattc tgccgatgat gggccgccct
     5701 gccagccgca ggtcgccgat gagatcgacg actttgtgcc gcacgcattc atcgggaaaa
     5761 cgaaaactgt ttttaatagg tccatccgag ctgataacca gtatttcctt agggctcaga
     5821 tgcgttccga ttcccattgc ctgcatttgg cgggcttcgg cctcaagcac aaacgtccgg
     5881 gtgcgggcca gatgctgtgc aaagtgcgat tcgctcaaat cgcaggaata aatctggcgc
     5941 ccgatgccgg tatgttgggt atagtccaaa tcataggtga tgctcaatcc gccggtacca
     6001 gacggcaggg catagatgga tttatcgccc tcatttatcg ctatcggctc ggtgataata
     6061 ataggattct gttcggcctc ctgcatttgg atgccgcatt tctggagcgt tttaaaaaac
     6121 tcttcactgc tgccttcaaa gcccggcagt tcaggggcat ctatttcaat aatcaggttg
     6181 tcaatatcca aagcgctgac ggcagccaga cagtgttctg aagtttcaac ataggcatcg
     6241 ctattttgca gagcagtccg ccgctgtcgg acggcaatgt tctgggggga caccggaata
     6301 tatacagggg gctgaaggtc tcggcgcaca aaaacaactc cggtatcggg atctgccggc
     6361 ttaaagacta ctttgacatc acagccgcta aagagtcccc gaccggatag tttggcttca
     6421 cttttaatgg ttttctgctg actcaagctg tttgaccctt tgtctaagtt ccttgagttc
     6481 tttggccagt tcgggaaggc gctgatagat tgctacacac tttagttccc gatgcatttc
     6541 ctgcggcggc atacccaaaa cagcctgtcc gggcggcaca ttttcggtaa cggcactttt
     6601 ggcacagacc atcgcgccat cacctaaaat tatattatca ataatgcccg actgaccgcc
     6661 cattgcaacc ccgtttccga gcaccgtgct gccggcaatg ccaacctgac cggccagcag
     6721 gcagagccgg ccgatccgga cattgtgggc aatctggacc agattatcta ttttagttcc
     6781 cgctccgata atggtattgc cgaatttggc cctgtccaca cagctgtttg caccgatttc
     6841 cacgccgtct tccagaatca cgccgccgtt gtggggaatc agacgatgct gtccatcaat
     6901 aaaggcatag ccgaaaccgg tcgaaccaat ggtagagttg gcctgaatga tgcaaaaatt
     6961 accgatttga caattgtgat agacaaccac attggcatcc agccgacaat gcatcccgat
     7021 aacggtcttt tcgcctatct tgcagccggg ccctatgact gtgtgagcac cgattttgac
     7081 accatggctg atatatgcac ccggaccaac agcagcagtc ttatcgatct gtgctgacgg
     7141 ttcgattaca gccgtcggat gaatcccgcc aaaaccggcc aactttggag caaacaactc
     7201 catagctgta ataagcgcct tgtttacatt atcgaccacc aattgcggga ttaggcattc
     7261 cggcaggggt ttatcagtaa ttacagccgc cgcacgagaa ccggctaatc ctttggcgtg
     7321 tttggctgat gttaaaaagc atacctgatc tggggcggca tcctgaattg tattgacacc
     7381 gactattgtt ttttcaccgc cgccggtcag ttttgctttc agcttttctg caagttcgga
     7441 caataacata tataggctcg ctgcaaaagt cattcagccg tcattgggcc ttatccagtg
     7501 cggccaaaac ttcggcagtc aggtcatact gttcgctgcg gtagagcagt tttttagtct
     7561 tgactgtaag cataaaatcg cgaagggacg gcgccggcag gtccagctgt tcatcggcga
     7621 tgaccatatc cagtcctttg gtcttggcaa catcctgaac gacggtcagc agcttttgat
     7681 agagcgattc tgtccagcgc tgcatttcgg attctacctt tgtgcggtaa aactcatctt
     7741 tggcatccat cagggccttc ttttcgagca tttcactctg aagacgcagg gcatcctcgc
     7801 tgccgggtgt tcgcagctta aggttggcct gcagagcatc cagttcatcc ttcatttttt
     7861 tgtattcgtt ttccatcgcg gtccgctcgc tctgcatttt ttcctgccat tgtttgtgtt
     7921 tggcacagtt ttcgagcacc tgtgttacat tgacgacccc gattttggcc ggtgctgcag
     7981 cggcggctgc gtcaagatgt gcctgccgat aaattccaag tgagaccaca acagctacag
     8041 cggagaacgt atacatcaat tttgcgtttt tcattttgaa tcctttcttt ctgctgggca
     8101 tctgaagatg cgccataaag ggtcaacttt caatcaaaaa acgaaaaaac ttcacgcttt
     8161 acacacgtct gcaccatatc gcatctgctt tcctatcatc ggccggactt agaagagagc
     8221 accgactgaa aaactgaaga cctgagtatc atcgtcttgg tctttggtaa tcggggcggc
     8281 cagctcaaaa cgcatcggca ccggcccgaa gaactggggt atcaaaatct gtatgccggt
     8341 acccacggag gagcgaacat ttccgtcatc aatgaggccg gcatccgtaa agaacagcca
     8401 cgaaaacatt tggctgccca gcggcactgc cacctcggca tttccgacaa ccatccagtc
     8461 gctcccaatg ggctccttgc tgggaccgct gcgggggctg acgccgcggt attcaaaacc
     8521 gcgcagcgag ctttgaccgc ccagatagaa ccgctcaaat gtaggagcat tgccgaccgt
     8581 agtagcagca gtgattttgg tttcgagcac cgttttcagc tcagccaagt cttcatagag
     8641 ggtggtgtac cagcgatagg tgccgtcgag aatgccaaat gtatggtcac cgaagacctg
     8701 ctcatagccg gcgtcaaaat tatagccgcg gctgggtgta aaacggctgt cggttgtatc
     8761 tttgcggata tacagccgcg taccataaag catattggaa cccttgacat caattacatc
     8821 ctgaggagca tcccaatcca aattctcaat tttaacatct tccagccgaa aactaaggcc
     8881 tctgcgccag tcatcggcat aacgtttttc cagaccaaag cgtccggtca ggcgcgattc
     8941 atcgtatgtt tcgcgccagc gttcaaagct ggagccaacc agatttaatg ccattggcat
     9001 atcaaacaga taaggttcgg taaaactgac cgaataagtg ctgacttccg tgccggggct
     9061 ggctgtgagc cggaactgct ggcctgcgcc gcgaaaggcc ttgccggtaa acagctcgct
     9121 ccagctttcg ggccagtcgg ttatgtcaaa attgcgctgg gtcagcgaaa tgcttccgat
     9181 gacgccgctg tcgctggcta taccggcacc cagcataatc gaaccggtct gtccttcgcg
     9241 aatggtgacc aaggcatcac gggtattggg atcggtgccc gtaggaacaa tagaggctgc
     9301 ttcagctaca accgactggc gaataatttt ttccaactcc ccttcgccgt tgccccgggc
     9361 aatatccgca ttgtaccatt ggccgggtgt aaaaccctct tcatccataa tgcgccggat
     9421 agttcgatcc tgcagcgtgg tattaccaac gacggtaact tcgccgatgc ggtagcggct
     9481 gccctcttta atttcatagt ccacatccac ccgggcttcg ggcagaatgg tacgcttggc
     9541 atcaacctgc gcatcgatga acccctgctg gccataggtg ctcttgattt ttttagcatc
     9601 aaaatcagcc caggcctcgc tgtagaaata attttgacgc agcttcatat tgccgctgag
     9661 ctgctcactg gagaaaaatt cattgccgga caggcggata gcattgaccg tataagccgg
     9721 cccttcggcg acatcaaatg ttacataggc cctgcttttg gtttcgttga acaccacttt
     9781 gtttgaaatc tgcgcatcaa gaaaggattt tttgtggtag gcttccagca gcttttgggt
     9841 atcctgctcc acctgctcag gggtataata gacggggaaa aagaggaact tacgcggctt
     9901 ggttttaaca acatcgctga gctggccgtc ccgcagcatc tggttcccgt tatactctac
     9961 atgggcgatt ttcggcctgg ggccttcttc gatctgatag gttacctgtc ccagcagaag
    10021 gccttcttct gaaactgtta cctttgccca agggaagccc tttttgcggt agagggcctc
    10081 catggcatca gcaccggccc gcaccagaaa cacatccaag taatcgccgg tgtgaaagcc
    10141 cagttccttc agcagcacgg catctttgag tttcttattt ccgactagcg caagagaacg
    10201 gaccagattg cgctcctcta caacatatgt cagcacaacc gttccatttt caatgcgcgt
    10261 gttgtagtag accctttcga cggcctccag ccggccgata cggcgcatgt cctcagcagc
    10321 ggtttcggcg cggaacggca ggccggaccg agagcggacg acagccagaa tttcggcacg
    10381 ggtcagcgtt acattgccgg ccacctccag acttttaatt gtcagacctt ccggagaaag
    10441 ggcagagtct tgtgcccccg ccaagccagc gcagatacaa acagccagca aaaccgccgc
    10501 ttgagaccgc aactgccgca tgttcacctc cctataagtt ttctgcatac cattaaaacg
    10561 gttctgtaat gtgacttttc ggcttaaagc gcgtcagacg cccgtcaaaa atcagctcaa
    10621 cagtaccagt agggccgttt cgctgcttgg caacaatgac ttccgccaga ttcagtttgt
    10681 cctgattggc actcatccac tcctcatccc cctgatgata ataggcctcg cggtgaagca
    10741 gcataatcac atcggcatct tgttcgatgc tgccgctctc gcggaggtcg ctcatcctcg
    10801 ggcggtgccc ttcgcggccc tcggcgccgc ggtttaactg actcaaaaca accaccggca
    10861 catccagctc gcgggccagt cctttgagtt ggcgggaaat ggtactgatt tcctgctggc
    10921 gggattcaac atagcccccc agactcatca gctgcatata atcgataaag accgcctgaa
    10981 tatcatagcg gcttttaagc cggcggcact tggctcgcac aaccagcgga ttcagattgg
    11041 gcgtatcgtc aataaaaatg ggtttttcat aaagttcgcc gccgacacgc atcagttcgg
    11101 ccatttggtc ggtagagagc attccctttc ggaccagctg ggaatcgatg ccgctgcggc
    11161 tgcatagaaa ccgctcgaca agctggtggc ggctcatttc cattgaaaaa attgccacag
    11221 ggatgccttc atcggcaccg agcgcttcgg ccatattcag tgcaaaactg gttttgccca
    11281 tgctcgggcg tccggcaata ataatcatct cgcccttctg gagacccgcc agcagatagt
    11341 ttaattcttc aaaacccgtc ggcagacctg ttacatggga gcctttgcgt ttctcgatat
    11401 tctcgaaggt ctcggtaatc agatcacgga ccggataagc cgtaccgcgg attttctttt
    11461 ctgtcactgc aaagacccgc tgctcggcag aatcaagctt ttcggaagct tctccttctt
    11521 cccggcaagc ctcgttgaga atttctccgc agacgtaagc cagctggcgc agcagggctt
    11581 tttcctgcac aattccggtg taataggcca cattggccgc cgaaggtact gtctcagcaa
    11641 cacgaaccag atactcaaca ccgccgacgg cctcaagaca gttgcgcttt ttgagttcat
    11701 cccgcagcag gagcaggtca atttcgctgt ttttttcata cagcgagaca atggcctcaa
    11761 aaatgagacg gtgctcggtc agaaaaaaag catcggcgcc gatggtctca atcacatccg
    11821 caatcgcctg ccgatccaag agcattgagc cgagcacagc ggcttcggcc tcaggagaag
    11881 ccggaatttt tcgtccctga agaagctgct gcaaatccgc atctgtccag gagtcattcc
    11941 tgggtatttg tttctttttt atctgcgtca acagtctgat cctctgagac gacaacgacg
    12001 cgaacagccg gccgcaggtc cgatgcaatt ctcagcacga cctcatgtgt gccgatttcc
    12061 ttgatatgac cggccggcat tttcaccata tcatcctgca cctcaaaacc ctgctgacga
    12121 agggcatcgg caatatcccg ttccgtaaca gagccgaaca gatgccccat ttcgttggcc
    12181 ttagcagcaa taatcacttg agccccttcc atacgggcgg caagctgttc cttctgctgc
    12241 cgttccagcc ggcgaatttc ggcccggcgc tgtttttcgg cttccagcga cttaatattg
    12301 gcctctgtcg gaatcgttgc aagtccctgc ggcagcaggt aattgcgggc atagccgttt
    12361 ttcacttcaa ccacatcgcc cagccatccg agtttttcca catcttccac taacagtact
    12421 ttcattatag tatctctcca ttaattttcc atagaaattg ccgtgttgac gaaagcggct
    12481 cggacttaag ccgtaaacgg cagcagagcc ataaaacggg cccgcttaat ggcggctttg
    12541 actttccttt gacagctggc acaattgccg ctgcgccgtc gggaaaaaat cttgccgcgg
    12601 ttggtcagca gcttctgcag ggtctcgaca tctttatagt cgatatatgt cttgccttcc
    12661 cggcagaaac ggcattgggt ttgctcgcga atcgtcgcgg taattttccg tcgtttcgta
    12721 tttggattgc tttgagattt tgccatataa aatcctgttt attaaatgtt aactgttttt
    12781 tcttcctaat tcacaaaagc catagtgcgc ggccagcata atgatgagtt tagaacggga
    12841 tatcatcctc tgccggaccg tacgaagttt cgggagcctg ttcgtaatcc gaagattcgg
    12901 ctggactggc accggccgct tggccgcctt tgccgataaa ctcgaagttt tcgacaaaca
    12961 ctctcagctt gctctgtttt ttaccgtctt tttcccatgc atcgtattgg aggcgtcctt
    13021 ccacaaaaat cgggtcacct tttttaaagt atttgtttac aacctcggcc cgtttaccga
    13081 acaggcggca atccacatag cagggctcat cggccattgt gccatcctgc tttttatacc
    13141 ggcggttaat agccagaccg aactctacca ctggcgtatt gctgggcagg tatgacagct
    13201 gcggattgcg ggtcatattg cccatcagca gcactttatt caaatttgcc attgtttaat
    13261 cctttctttt gcagctatcg gacgcttcca attcgccttg tgggcagata ttaagcggtt
    13321 tcggcagttt cttcagcgac atcggaagtt tcttcggcat tgttttgctc ggcgggctta
    13381 tccgaagtat cctgtgccgg ctgcatttcg gcaggggtcg gcttttcgat atcctgctgg
    13441 gtcattccgt cagtccgcag tatcagcgca cggagaattc gttcggaaag ctgaacatcg
    13501 cgttcaatgc ctttcacttt atcggtgggg cagttgaaat acgccagaat ataagttccc
    13561 ctgctctttc gctggatttc gtagcacaag cggcgttcat cccacttttt gagcgaaaca
    13621 atttccgcat cggcgcgctt taaaatatgc tgcacttcat cgagaatgcc ctgccaatct
    13681 tgtgccgcca gcgcagaatc gaccaggaac atcccttcat acaatcgttt tacagccgtt
    13741 tccaaagttc atacctccca ttgggttcat aggattttaa tatttcatat ttttttctgc
    13801 cgccggacac agtgcccggc ggcttgattc acatcgcaga aggcggctgt ccagaagcat
    13861 cgttcttctg tggactgccg gcctctgttt gaacattgta ccgattcatt gctgcgtcaa
    13921 tgccttctgc ggcccagcac aaggcggcac cggccgctgt gtgaaccgct tgggctataa
    13981 tggactgctc ttctgcggcg aatttcgaca gcacatagtc ggccgaatcc atcgtaccgc
    14041 tgggaccgat acccacacgc agtcgagcaa actgatccgt accgaggcgg gcgataacat
    14101 ctttaagccc gttgtgacct ccggcagagc cttttgcccg cagccgtatg cgtcctaccg
    14161 gcagagccat atcatcggtt acaaccaaaa ttttatccgg ctggagctta taaaacccag
    14221 ccgccgaagc aaccgaatgg ccgctgcgat tcataaactg ctggggctta agcagcagca
    14281 ggcggtatcc ttgggccgac acctcttcga acagggcatt aaacctgctg cggctgaatg
    14341 cagcacccag ctgctgggca atggcatcca aaaccataaa gcccacgtta tgccgcgtgc
    14401 cggcataagc ggcacccgga tttcccagtc ccgcaatcaa tttcacagca ggtgcttctt
    14461 ttgtttccaa agagccgatg cccgtcaggc atccaaatac ttcattccaa tcttttgtca
    14521 tggctgcctt atccccatga acatatccat cgggagctat caggaggcgg cttcctcagt
    14581 gggcttgcgc tcggtaataa cttccggctc cgtcggggct tcagcacctt cagcaggagc
    14641 agcttcttcg gccttcgccg ctgcggcctc atggcaggtt acaacaaccg cttcaggatc
    14701 tgtaataagc ttcatccctt cgggaagctc aatctgacca gcgtgaatgg cctgattgag
    14761 gccaaggtgc ttgatattaa ccggtatcac ttcggggata tcactgacgc ggcattccac
    14821 ttcgaggcgg tccagaatca attctaccat tccgccctga tgcgtccctt cggcagtgcc
    14881 tcttaattcg accgaaacct caaccttcac ccgctcgctg agatcgacgc gcatcaggtc
    14941 tgcatgaaca atatctttgc cgaggtaatc gtactggagt tgtttgacca gcactgtctc
    15001 ttttcggccg tctgtatcca gctcaaaaat acgggctccg ccgtgcagcc cttcaagaaa
    15061 atcgtgggca tccagcgaaa tcgacaccgt ttccttttta tgaccgtaaa gaacagccgg
    15121 aagctgtccc tgccggcgca gacggactgc ctcattggat cctgcctttt ggcggatgcg
    15181 tgcttttaac accgttgttt ctgacataca tacctctttc ttagttctga tttttcgtgt
    15241 ttgaaacgac tacagttcgc cataatgctt aaacatcgtg ctgatggatt cgttgctgtg
    15301 aattcgccgc atcgcttccc cgataaggga agcaacactg agcaccttca gatgcggcag
    15361 cttgcggacc gattccttga ggggaatcgt atcggtcaca atgatttcat caatgggagc
    15421 ttctttcaga cgctccaatg ccggctcggc aaaaacccca tgagtcgccc cgacaactat
    15481 ctcccgggca ccaagagacc ggactttttt ggcagcgccg gtaatagtgc cagcggttgc
    15541 aatcatatca tccaccatga caatattgcg gccttcgacc gaaccgatta tttcctcgca
    15601 gagaatttct tgccccgtca gacgccgctt gtgaataata gccagctctg cgccgagtgc
    15661 ggtggcatac cgcgacgcgg tcttcatatt gccgacatcg ggagccacaa ccgtcagatc
    15721 ggtcatcttt ttgcgcttta agtactgcac tatcaccggt tcggcagtca gatgatcgac
    15781 aggaatgtcg aaaaagccct gaagctgctt ggcatgcata tccagcgaca gaacacggct
    15841 ggcgccggcg gatgtaatga ggttggcgac caattttgcc gtaattggca cgcggccggc
    15901 atctttgcga tcttgacggg catagccgaa atacggaatc accgccgtaa tccgggcggc
    15961 gctggcccgc cgcacacaat ccagaaaaat cagcagttca accagatttt catcaacagg
    16021 agcacatgtc ggctggatga taaagacatc gcggccgcga acatccgatt cgagacgaat
    16081 cagtttttcg ccgtcgggga agcgtgttat ctctgctttg ccaagttcga tattcatata
    16141 ccggctgatg gccatagcca attccggatt gctggttcca ctgaatataa tagttggatt
    16201 attcagcatt gtctgctatc cctgttaaag ttgtttgaat acgcccacaa cagagccatc
    16261 ggctacaacc gcgccttcct gcaggaaggc aaagggaccg atacggcagc cgctgccgat
    16321 atgaacagcg ccgctgatgc aggtaaaagg ttctattaca gtatcctgac cgatttttgc
    16381 acggtcatca atccaagtgt tgggcggatc gacaattgtt acgccagccg tcatcagacg
    16441 gttttgaatc cgctgctgca taattttgct gaccacggca agctgctggc ggttgttaac
    16501 gcccattgca tcctcggccg ccactgcagt aacagcggtg gccttatggc catcagccaa
    16561 aagtatatgc agagcatctg tcaggtaata ttcattttta gcgttgttcg gcttaatttt
    16621 atcaagtact tccagcagat attgcgtttg aaagcagaaa tagcccggat tgacttctgt
    16681 gatcgcgcgc tgtttttcgt cgcagtcgtt gtgctcgaca atgccctgaa tattgcccca
    16741 gctgtcgcgg atgattcgcc catagccggc gggatctggc aagacagccg ttgctaaggt
    16801 aaccgccgct ttttcctgac ggtgcttctc gagcagaaca gtaagggttt cggtgcgaat
    16861 cagcggtgca tctccgcaaa ggaccagcgt ttggccggta aatccctgca gcttgtcccg
    16921 gcagcacatt actgcatgac cagttccccg ctgctctttc tgctccacaa actctacatc
    16981 gcttacatcc cgataacgtc cgataatctg gtcttttccg tagcccacca caacaatatg
    17041 tctttggacg ccggcattgc ggcaggcatc aatgacccac tcaagcatag gccgtccgca
    17101 tacctcatgc agcaccttgg gaagccgcgt attcattcgg gtactttgtc cggccgccag
    17161 aataattgca attttgtcaa tcatcagaaa tttccaaaaa tactgtaaaa ctctacccgg
    17221 cctggactcg aaccaggaat agaggtacca aaaacctctg tgttgccaat tacaccaccg
    17281 ggtattaaaa acagccgata tctctcacgt ggcgcaagcg caaagttaca acaaaagccg
    17341 actgtctgtg tcgtccgtat tagctggtcg cagaatccgc ttcagaaaac ggattgaccg
    17401 ggccctgccg gaggcatgag ctgaccttgc tgcgatacct gtcagcccca tttccaacgc
    17461 tgtaaaccgc aatttcagcg atttttcaat tctttcgtcg caaccccatg cgacagggca
    17521 taaatcaagg caataactat aaacttaaaa tgccataaaa tgcaagagat tttttaaaaa
    17581 tttggcgaaa ggcataccat ccagcctctt tttgattttt aatctattgc aaaaaaggca
    17641 atgaatttcc caatacaaag ccggcttatt gggcctgcaa tataagatat acaatatata
    17701 gtataagcag aatcccgccc tgccagcgtt caagctggtg acgttttcct atgaacatgc
    17761 agaaaaacaa agtcgcagcc gaaaggatgc ctatccacag cccaatcatc aatgtcggcg
    17821 caatcggcag cggccgaagg accgcgctga tgcccagtat tagaaagata ttaaatatat
    17881 ttgagccgac gacatttccg acagctatat ccggtttctt ttttactgca gccgctactg
    17941 aggcagccag ttccggcaaa ctagttccga cggctacaac cgttgcggca ataaaagcct
    18001 cactcaaacc ggcggcggcc gccaatcgga cagctccttg cactactgct ctgccgccgc
    18061 caataaggaa tatcaaaccg gcagccacca tcaccatcgc ggcgggcagc gagacagact
    18121 tgaaatgctc atgggtattg tctaaacccg gtaattccct tgcaataccg acaatgtagg
    18181 ccaaaaaaac cagaaaaaga ctaagcagca gaatcccttc aatacggctt aactgagccc
    18241 ctgccgcaca gaaaaacgcc agaacaatag aaccccccag acacattggg atttctttcc
    18301 atactgttcc cctgcctacc tgtacgggcg caatcaacga cgataagccc agaataagca
    18361 atatattggc aatattgctg ccggttatgt tcccaacagc caccgccggc tgattctgca
    18421 caactgcaaa gatattgacg gtcaattcag gaagactggt tccaaatgca acaaccgtca
    18481 aacctataac caagtccgat acccggagac gacgggcgac agccgatgcc ccatcaacca
    18541 gtaagtgggc acctactatg actccagcca atccaaaaat cagcaacagg gatgcaacca
    18601 tttaaggcaa tcctttctta tatcagactt gctgttatta agattgctcc gctgttactc
    18661 cttgcgtgaa atacaggccg gactgttgag aaaatccata acatcctgac gtacttggtc
    18721 gagcttgagt ccaagattgc gaaggacttc tgtggcgata cactcttttt catacaacaa
    18781 acccaacagc agatgctctg tgccgattgt tgactgcttg agcattcgtg cttcattcac
    18841 agcatcttgc agtacacgtt gagcatgctc tgtgcagggt aatcggtcat aatctccttc
    18901 tggagatatc gaaccgggta atacgagttt tcggacctgt tctttggcct gtgataggtt
    18961 tacgctgcgg tgtttgagaa cataagcggc acagccctct ttgacctcaa gcaaacccag
    19021 tagaatatgt tccgtaccca gatgatcgtg accatacctc tgggcttctt gacgggcatg
    19081 ttccattacg ctgcgggcat gttcggaata atgttcaaac atcgggcacc tttttcacaa
    19141 aaatatctct attataggtt gaatggaatc gctatcctca tttgtcaaaa gcatacagtc
    19201 aatttagtat aaagtctaat aaaactatta aaagtgcttt tgcagaatat acaaagcaaa
    19261 aacacattaa aatagttgtg atataattca cataataata ttgctatact tatttttatt
    19321 atttatataa accttattag tgttttattt cacaataaat aattttaatt ttgttatata
    19381 gatatcatta tttctgcttg acagggtaca acaaaaatgg tagttatctt tttaagcttg
    19441 tgaatgtgaa cacaaccaca aattcggctg attatcattg ctacgtagtg tattagttat
    19501 gaaataccga aaaatcccgg atgagacgat ccggcggatg ccgatgtatc tgcgagcttt
    19561 ttcgatgctg aaaacggagg gactggaaac gatttccagc cgtcagttgg cccagcggct
    19621 tcatttgaat ccgcctcaaa taagaaagga tttatcttat ttcgggacat tcggcacccg
    19681 cggcatagga tatccggtag cagcaactgc tgaccagatt cgagatatcc tgaagctgaa
    19741 tacgccccaa aaggccgcct tggttggggc cgggcggttg ggcagcgcgg tagcggccta
    19801 tccgggtttt ggaatgttcg ggtttgaaat agccgctgtt tttgacaatg ctgccgacaa
    19861 aatcggacag aaaatcggcg gcattaccgt agaggatgct gaaaaaatca gtacaatccg
    19921 gcaacggggt atttatctgg cgattttggc tgttccggct tatgcagcgc agtcggttac
    19981 agataaactt gtgaaggcag ggatacgagg gattttgaat ttatcgccct gctatctgga
    20041 cgtgcctaag cggataaagg ttgtaacgat tgacattgca acggcgctgg gtatgctgcc
    20101 gtattatatg taatgccggc ctggctcggc ggtgctctgt aatgctgtca ggcagaaaaa
    20161 caacaagcaa caacagtaca ggaaagcaaa aaccatgagt gtaagcacag taaccaaaga
    20221 gcaattcaag tcattcgttg ccggactaat cggctcggaa caaatggtta tcggcgtgca
    20281 ggccaaacag gacaaatttg cgtttggtcc gctgctgcgg gcagaagacc tgcgtctgga
    20341 ttatgatgtt accattctat cgccgcggaa atatcttctg ccgccccgtg aagttctgct
    20401 gaattatcag gtctgcggcg aggcgcaaag catctgggac tgccggccga tgattctcat
    20461 cggcgtgcat ccttacgata tgattgccat caatcagatg gatgaactgt tccggcaggg
    20521 tcaatatgat gcccattata tgaatcgccg caacaatatc acgattattg catgcgatgt
    20581 ggctaccccc agcatgaatg tattcgcttc ttcaatggat accgccactg ttgagagcgg
    20641 ctttgatatt ctgctgacgg atatcggcag cgggtacgtc gtgcaggccg acaccgacaa
    20701 aggccaagca ctggtgaaga aaatggccgg tatcaaaccc gccactgcgg aggacttggc
    20761 caaacgacag gccattcagg acaaaaaccg caaaacgctg aacaaacacc cgctcgaatg
    20821 caagccgtct tttatcccga aacttcttga aaaggcctat gatcatccaa tctgggagga
    20881 gaaggcccgg ctgtgctatt cctgcggctc ctgcaatcag gtctgcccga cctgctactg
    20941 ctttgatgta caggacgaag taaactggga tcttaaaacc ggcatacgtt accgcgcctg
    21001 ggatgggtgc ctgctggaaa acttcgccac cgtagccggc aatcacaatt tccgaaaaga
    21061 ccgctctgcc cgcttccgtc accgtttgta ccgcaaaggc aagtatgtgc cggccaaaat
    21121 cggggggcag attgcctgcg taggctgcgg acgctgcatt acagcctgca ccgccaagat
    21181 tgccaacccc gtagaggtgt ataacacttt gctggaaaaa acagatatcg gctgatcctt
    21241 actgactgaa acacaataaa ggagattata cagatgtgca actgctgcaa cggcaaaaac
    21301 gatatttatc gtcccgagct atggacgatt accaaacacc gaatgctcaa cggcacagag
    21361 atgtttatgc atctggagca ggattcgggc agagggttcg actatatgcc cggccagttt
    21421 gtggaggtgt ctctggccgg catcggagaa gctcctattt ccatctcctc atcgccgaca
    21481 cagaagggac tggaactggt tgtccgcaat gtaggcggtt taacacggtc tattcacaag
    21541 ctccaaaccg gcgataaact cggcatccgg ggaccctacg gctccaccta tcctgtcgag
    21601 cagacaaaag gcaaagattt ggtctttatc tgcggcggca ttggactggt gcctcagcgt
    21661 tcgtttatcc gctatgccct cgatcatcgg caggattacc gtcagattac cgttctgatc
    21721 ggcaccaagt gctatgacat gcggctgttc cgcgaggaaa ttgccctttg gcaggagcgc
    21781 aaggatatgg tggtgatgga aaccgttgat gaggcccatg actgctggga cggcaatgtc
    21841 ggagttgtta ccacgctgat tcccaagatt gaaagcgacc tgccctccag tgctgtgctg
    21901 gtatgcgggc cgccgataat gtataaattc gtgctcatgg cgctggcgga tgccgaagtg
    21961 ccggaggaaa acatctatgt caacctggaa cgccgcatga agtgcggtgt cggaaaatgc
    22021 ggccactgcc agattaacga caaatatgtc tgtcaggacg gccccgtatt ccgatggagc
    22081 gatttggccc ttgtgccgga ggcgatctga ttatgaccaa accacgagta gcaatttttg
    22141 attttgcctg ttgtgaaggc tgccagcttc agattgtcaa cctcgaagag gagattctca
    22201 atctcattga gctggtcgat gttgtcgagt ggcgtgaagc catgagcgaa caaagccacg
    22261 aatatgatat tgccattatc gaagggtcaa tcacccgcga ggaagacgcc aagcggctgg
    22321 aggttat
//
LOCUS       DAOTBO010000040        21329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2031_length_21329_cov_5.871721, whole genome shotgun sequence.
ACCESSION   DAOTBO010000040 DAOTBO010000000
VERSION     DAOTBO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 21329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21329
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2031_length_21329_cov_5.871721"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLE88_06335"
     CDS             complement(<1..517)
                     /locus_tag="PLE88_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76117.1"
                     /translation="MIRKTLNHRNIRYWALIVSVGVHSAALAFFTSVRLSGRISQAAV
                     KPAISLQAIEHIVQESGPRPKPQVEPIHTAAAPCEEQKPPMPSPADSAVPAQQSQLPS
                     ESAAQPTVPNEVEFFGQKSIVQRICYVVDCSGSMHGQMYRVREQLKESILKLTAHQAF
                     CAVFFMNGQTIL"
     gene            complement(583..1791)
                     /locus_tag="PLE88_06340"
     CDS             complement(583..1791)
                     /locus_tag="PLE88_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOM76118.1"
                     /translation="MTAREYRPYPTRKQQKDRSFRIIAIALIVLIAAAVIFIQQWKNK
                     QPHSVPDEVQKVSLDNVLPAKPAAAPAEPQRVLNPSVALKTESVPPAVPSPTPAEPAK
                     TAVSGTAVPTESVKPAEPAAVPKPAETVTTVQIPQDVLTAASADTGQTSEKAKEIIKQ
                     ATELRDAGKVIFARDLLNDALNMQLSPVIRSAVKLELSKLAQKWLFSPQIFPDDKLTG
                     QYLVQPGDLLQDIARDHKVPFEILMEINGISRPELLQAGKKIKVINGPFNVVVYKKSF
                     TMDLYLQNKYIKTYRVGLGKEEHETPAGRWRVAKGGKLIKPTWTDPDTGKRYLGTDPD
                     YPLGSRWIAIEGIDENTRNRTGFAIHGTKEPETIGTRSSRGCIRLHNGEVIEVYNLLY
                     PGISEVLIEE"
     gene            complement(1806..2378)
                     /locus_tag="PLE88_06345"
     CDS             complement(1806..2378)
                     /locus_tag="PLE88_06345"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOM76119.1"
                     /translation="MDKSALRQKLKQQLMQMSREEIIAKSKIICDYVIGSEVFQKASV
                     VMAFLSLPHEVDTTPLILAAWQRGKTVAVPKISWQQRHMIPVEIASLETGLKTDEHGL
                     RNPIGGIPVPFDEIDLVITPGLGFDAEGNRLGRGGSYYDNFFTSNKITAARWALAFSH
                     QICPAIPHDDSDVPVDAVVTENGIILCKRN"
     gene            complement(2397..3764)
                     /locus_tag="PLE88_06350"
     CDS             complement(2397..3764)
                     /locus_tag="PLE88_06350"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOM76120.1"
                     /translation="MTQGGNNLFSEGERKALEKQAPLAVRMRPAVLDEFVGQEHFAGP
                     DKLLRRMLEAGNVTSLIFFGPPGTGKTTLARIVAGSIQATFHYLSAPAASVKDVRDII
                     AQATDRLLTSKRKTLLFIDEIHRFNRAQQDVLLDDVENGIITLIGATTENPYFSVNSP
                     LISRSTVFEFQPLSKDAILTLLGRALADKEKGLGKMAVDADPEALEFLAEICDGDARK
                     ALTALEVAVMSQARSSTPIKLTVEIAKESVQKKAIAYDGTGDTHYDLASALQKSMRGS
                     DPDATVYWLARMIAGGEDLRFIARRIAVCAAEDVGNADPTATILAASALQIAEFVGFP
                     EAQLPLAQAAIYIACAPKSNACANAIWQAVSDVQSGRTIPVPPHLRDAHYAGAKKLGR
                     GLDYKYPHQSQTGYVEQDYLGQKLEKPYYRPKNIGREKIIAEYLQKLKNQVQQYQRQA
                     ESETD"
     gene            complement(3940..5052)
                     /gene="mnmA"
                     /locus_tag="PLE88_06355"
     CDS             complement(3940..5052)
                     /gene="mnmA"
                     /locus_tag="PLE88_06355"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOM76121.1"
                     /translation="MNHYKLELISSHIAPWEPKNSLSRQIAVLMSGGVDSSVTAHLLH
                     QAGWDVLGITMRIPTVCRTDKRGCCGAEAAYVCAQLKLPHYFVDVTAPFNELIIDRFR
                     TEYRRGRTPNPCADCNRLLKFTLMWDLVEKEFGIRHLATGHYAQVLHTPHGPRLRRGH
                     HHAKDQSYFLYGIPRRRLADFVLPLGQMTKEEVRTIARNIGIGVAEKSESMELCFAGE
                     GNYRAALLEGDKPGNLLDIRGNIIGTHKGIAHYTLGQRRGLHYAAGKPVYVAGIDAEH
                     NTVTLGTREEISHTIITAGEVNILIPEQIKTGQTLFGKLRSYGPVHQCQITEFDDIHL
                     TVRFEKPQFAPCPGQKLVLYNTDEDIVAGGTIEAFQ"
     gene            complement(5049..5987)
                     /gene="miaA"
                     /locus_tag="PLE88_06360"
     CDS             complement(5049..5987)
                     /gene="miaA"
                     /locus_tag="PLE88_06360"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOM76122.1"
                     /translation="MSSKTQKILILGVTASGKGALAHELASALGGEIISVDSMKVYRR
                     MDIGTAKPPLEKRRLIPYHLIDVVEPSDSFSVDRFLSLAEQAVADIQSRQKTVVAVGG
                     TAMYIKALLYGLFEGPGTDENIRQKLKQQAAAEGLELLHKRLAAVDAEAAGRIHPNDE
                     KRIIRALEVFELTGKPISHFQKQWNTPHPPEGWQIFGLRRPKETENRRMNARVKRMVD
                     QGLREEVAALLAEPKPLSKQASAAIGYAEMIAHLKGQIDLDEAVEQIKINTRRFAKAQ
                     RTWFKTFRGIRWLDIEQEGTVQTILEKVLELMDTQV"
     gene            complement(6113..7072)
                     /locus_tag="PLE88_06365"
     CDS             complement(6113..7072)
                     /locus_tag="PLE88_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOM76123.1"
                     /translation="MILAGLLIGGLVFLYAGAELLVKGAVAIAQRLGVGPLVIGLTIV
                     AFGTSMPELVVSIQAAQDGAGDIAAANVIGSNIFNIALILGLAAVICPIRITAQLVKV
                     DIPILIGVSLLCILLLADRHLSRLEGAFLFAGIIAYTVFAFYQARQKPPQTVLQEVEQ
                     MLSRQKTSQLTPFGLPAAGAIAGGLVLLVAGSKMLVSGSVRLAHLLGLSEAIIGLTIV
                     SAGTSLPELATSIVAAVRRQPDISVGNIIGSNIFNILAILGLSSLLMPYSAPGITNVD
                     LWVMTSITALCLPLMWSRFILSRLEGGFMLICYGIYLAYLWPK"
     gene            complement(7077..7721)
                     /locus_tag="PLE88_06370"
     CDS             complement(7077..7721)
                     /locus_tag="PLE88_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOM76124.1"
                     /translation="MTAAITNHLKRRILSGFFVVLPLFITAVILKFLVGIITDMLSPV
                     LLWIFLNIPVWIKTHVSLAILIAAIYLLGLTTGHLAGRWLVNRIDQMLLSIPFLNAIY
                     GSSRDVVRIFLNPERQAAFKEVVLVEFPAPGLGAFGFITGQIINPEGQSCYKVFIPTT
                     PNPTTGFLEVIEQSRVIRCPMTVEEGIKIIMSGGILGSETLQPKINSMERKQET"
     gene            complement(7784..8251)
                     /locus_tag="PLE88_06375"
     CDS             complement(7784..8251)
                     /locus_tag="PLE88_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76125.1"
                     /translation="MSSTHVSLQDKIGYIRELLSSDQTEKALLFIDKNGTQNQEMKNA
                     LGVCLMRAGRIEQAVTVLRDLVFQNHICIPDETPPLYQANYATALLLQNYNQMAIEII
                     KRIPVNSHPYVAQLHQAIGQWQKSLPLLRQMQCLLGLYPSGCRVPMDFKPGCI"
     gene            8883..9800
                     /locus_tag="PLE88_06380"
     CDS             8883..9800
                     /locus_tag="PLE88_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOM76126.1"
                     /translation="MDIQTSQNQNNPETFKASLADKANLTGQVLAELLGQQDRIPVRL
                     REAMDYMLQSGGKRIRAALVMWTCETVASKLNRDAQIAAAAVEMVHTYSLIHDDLPAM
                     DNDDMRRGRPSCHKQFDEATAILAGDALLTLAFEILSEKIEDAEIAVRMIRTLSRAAG
                     PSGMIAGQAADMQYANTGGTLASLEYIHTNKTGAMFAASAALGAIAARASDRQIQTLL
                     EYGMKIGLGFQIADDLLDITATSTQLGKTAGKDAAQGKLTYPALVGTEKSREIFEKLT
                     CQAIAAVEDFGPQADILRQLARELLNRTQ"
     gene            9801..10760
                     /locus_tag="PLE88_06385"
     CDS             9801..10760
                     /locus_tag="PLE88_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM76127.1"
                     /translation="MNILPHAVLSGMLSILPMAVGGCKVCSNRTPAALQFGVAADVQY
                     AQKPPAMGRHYARSLDALKRCVEEFNRRSPAFVIQLGDLIDGGENAQEDMREAVSVYD
                     ALVMPHYHLLGNHDFSGLPRSSTMCLLGLEQAWYAFDVQGWRFVVLDTQDLAVEGGWE
                     KSSLRYQQSLDMLIQLQAAGAANAQTYNGGLGSQQLVWLDAQLTDADRQGMPVIVFGH
                     LPLMPSDEKHIAWNAEQVVSVFEKHRCVRAYFSGHRHSGGYTLHNGIHYITLEAMVDS
                     ADTDGAWAIVKLAPQTIIIEGTGAVASRTLELPPHKAERRRLD"
     gene            complement(10757..11479)
                     /locus_tag="PLE88_06390"
     CDS             complement(10757..11479)
                     /locus_tag="PLE88_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HOM76128.1"
                     /translation="MAIMQDNLNCLKNLLRLMCCDGKIEPQEKAFLARAAQELAVPVK
                     NWRILLQTVLEDPVGCWPVDDHTKAAAALKAMIVMAKADGRVDEVEKQFVLQFAKAAG
                     IRKEEWKRLLSEIDVANLFAPFQSPAKTLMILKEDFDNLDAFLKTACDSGLTVQTADV
                     QGFLKAPAENDTIICFHASPDKDITLTRAVMLLEKTAPVLLVCILTRFQGHQVKYLHE
                     AGVKTCIIEPVYPKDLYGILCA"
     gene            11680..12171
                     /locus_tag="PLE88_06395"
     CDS             11680..12171
                     /locus_tag="PLE88_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76129.1"
                     /translation="MKKWLFIASVILTASFVLADDQPSRPSPDSPRPQRQEVLRPAAA
                     ERLGAVQRQQQERLDRLEQQLNHLIKRLESQAEPIPGPKAVVPPVKAIKPQRGPRGHR
                     PFWCAMMVCCAVVHILSAVWVFQDIRTRGAGSGVWIVIALLAGLFGVLVYAVVRLGDI
                     RKT"
     gene            12387..12875
                     /locus_tag="PLE88_06400"
     CDS             12387..12875
                     /locus_tag="PLE88_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76130.1"
                     /translation="MLALLNISGLELLFACCAVFGLILFVIRLILMFIGMGSEGLDSA
                     DAGHIDTGAMESPGQGGDFHSGLHDSDLSFKTLSLQGITAFFMMFGLVGWAVLRQGNY
                     AAWIPILCGAAAGAATVWVMKKIFQLAGSLQSSGTMDLQNAVGQEGTVYLTIRPGKSG
                     KV"
     gene            12961..14214
                     /locus_tag="PLE88_06405"
     CDS             12961..14214
                     /locus_tag="PLE88_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOM76131.1"
                     /translation="MAFRPENALSLQNIRINVPSTFTVGISTEPGIMNNAAERLLRLN
                     PHQIEEMAKEIIFGQLRLTVASLTIEQINQDRESFLEAIRKNVEPELNKIGLFLINVN
                     ITDITDSSNYIESIGKKAAAEAINTAKVDVAEQEKFGAIGKAQAVREQTIRVAENFAE
                     SEKGQKKAEADREKAIRIAENIAAADKGQKKAEADKRIYVQGQEALAVTGENKAKADI
                     AEAEAQLAIKRAEALQKGEVARRQAEVEIQKAQYLAEQQRLNAEVIVRQEIDKRKVEI
                     EAEALAERYRREARGQADAILAKYEAEAKGIRQVLESKAAGYESLVKSCNGDAKAAST
                     LLMIEKIEDIVARQVEAIKNLKIDKITVWDSGSDKGSSTANFLSSMIKSLPPLQEIAS
                     MAGVELPEYLGKMADKQNASGDAKR"
     gene            complement(14339..16177)
                     /locus_tag="PLE88_06410"
     CDS             complement(14339..16177)
                     /locus_tag="PLE88_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOM76132.1"
                     /translation="MKTKLFIGVLMVLMTSPLFAEPQLAESRISSVIVYHGQALVTRT
                     IQLDHPGGDLELLVENLPERILPETLYAQASDGIQVLSVRYRIKEIEKDNSKEVKELE
                     DQIYTLERQLRDTVRSMDNANDRLDRYREQWKFITDTSKVEMTHGVLNAPTIIDLNVF
                     MASKNSEIHKELVILENQKLDLEKQLAAVREKKDKLVTEYKRQIRQALVYVHVPAVGA
                     ADIVQLSYLVDSADWSPQYNLKANPAHQNVHIDYNAVVHQSSGEDWIGTELALSTAAP
                     ALVAAPPVLNPMQITLTGAGADKRGLPAPSAVPQPAVSQRVEPLLGEPFQQPTQQQES
                     AYSYGDQTERFAQLAQSRRASIARGIKASAELNQIAIYNQMIEFEADKRLLQQIKERT
                     VALQRAEGVSVIYKLPGKLTLPSKSDQQLVTIAAIDCPAVFTFAAAPLLTDYVYLIAD
                     ITNTSNTILLPGAGDIFRDGEFVGKADIKLVTIGQTFTAGFGVDSQIQVAREFKDKKI
                     ETLWGNRVDQQEYRIEISNYKNEPAACRLLDRLPWTENPALFIELTAVSHPLSQDADY
                     LRTQKDKGILRWDLTLKPETSGKNATVINYTFTMKYDNDMVIRPMQ"
     gene            complement(16218..18065)
                     /locus_tag="PLE88_06415"
     CDS             complement(16218..18065)
                     /locus_tag="PLE88_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76133.1"
                     /translation="MNKHIRLVLTLRILVLIELSGAVLYAVPEQPAKKIYGKLPVKEV
                     TIFKDGHAYILHEGKMKTDSQGNIPLDSLPNPVMGTFWAYSAEPKAVLTSVVSSREEL
                     DAECTALTLEDLLKGNLGKNTLIKEITSNAPWQGTVLRILEEKAFPPSSQIDPALNAY
                     SYQPPQSQSREKIVLLKAADGIRAIPISQIQSVTFLDNPSDTVSRKEIKETMTLHLNW
                     NGRAPAAEAAVGMAYVQRGIRWIPNYRVEIDGKGHAVLKLQATLINELADLNDVTAHL
                     VIGVPNFAFKDTPDPISFQETAARLSSHFRPDASTAYALSNAIMSQMRSSAAERRATP
                     APGDGATDLGQEFEGKTEDLYVFSLEHLTLRKGQRMVLPVAELTLNYEDIYTVDIAFA
                     PPLEMRRNFSSDQQLQLARLFHKPNAVHKIRLDNTSEYPLTTAPAAILKDGRMLAQGM
                     MTYTAVGNKGDLELTTAVNIGVKKSDEQTIMIPNAVNWNGNNYSKIEMKGTIELTNFS
                     DRSIKLQVRRSVLGNMDCADTDGIIKQLGNGYDGLVFEEGLPFWWSWCSWPWWWYRFN
                     TVGTAEWTVELSPKESKNPNLRLALFLGIKPAAGPGTGYAFTVGAEQRP"
     gene            18217..18918
                     /locus_tag="PLE88_06420"
     CDS             18217..18918
                     /locus_tag="PLE88_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM76134.1"
                     /translation="MMKTHQHILIVEDEPKIRDGLRDFLEFHGFEVTAAADGLEAERL
                     ADDKHFDLILLDLMLPHISGEQLCRKWRQQGLTAPVIMLTAKGQEHERIAGLDMGADD
                     YITKPFSLEELLARIRAVLRRTDPARSVGQTFAFGPFTVDTQALCLIAGGRRTPISPR
                     QAQMLVVFAACPNRIVSRRELYEKVWHESLDGIETRTIDMHIARLRELLKDAGAHEDL
                     IQTVRGIGYKYECEQ"
     gene            19019..21175
                     /locus_tag="PLE88_06425"
     CDS             19019..21175
                     /locus_tag="PLE88_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM76135.1"
                     /translation="MLKHFGIICLVVGMGLLILSGLGLSSLGMYKKGLAAERRQEFIA
                     VAEQVRYEIKQQLDAFLQAEQGRPWTDYQYAYVPESFNQAAALVRSPLADLFTNNLAV
                     GYFQLDSAGQIITPYSPPGQEVLMPQAAAYFQNIRNHLLPSLRLGLNLQPYRIDPSAP
                     SGANGAYGKLAAERIGSVAMTPSPDRSETAPTKKDMPAVKSPVPAEQKASAVKEPDMR
                     RGVYAVSPLEDIHQKTQVIRQSRAAYEMNVDNTQVLMGQAPVFGSAPLLDEAEQQPHS
                     QTRRPAAAESQRQVPQEAIPPSLPQSQTAGASMPANAFSRQQSQDPRQRPMDFQTTLS
                     AAEQMETVQVRIEPFVPITVPEPAGRNGIFAGQIFLLRHVQIEDRHWIQGFQLNEAEL
                     LRRVSDAVARLLRRGMDYEISRQERPDAAHSAILDFGFGEVVLNLLEQQPDWIQMQAR
                     RLAGWFWAILAVVWLAVAAAMAALWTNMRQQAHLSRKKDDFISAVSHELRTPLTSIRM
                     YTEMLENNWIKTDDKRRQYYTAMRQESERLSRLIENVLDFSRIQRNKKQYHFAVGDVN
                     DPIRQAAQMMRPCIEQAGFVLVEQLDEIAPFMFDRDAVMQIVVNLLDNAVKYARTAED
                     KHIFLRSRQVRDRIFIEVEDRGPGVPRSQQKKIFDAFYRCEDESTRQTAGTGLGLALV
                     RQYAQAHCGNVEVAAVQPSGALFRVMLDVRTVKSSG"
BASE COUNT         5042 a         5276 c         5650 g         5361 t
ORIGIN      
        1 ccagaattgt ctgcccgttc ataaagaaaa ctgcacaaaa agcctgatgc gcagtgagtt
       61 taaggatgga ctctttcagc tgctcccgta cacggtacat ttggccgtgc atactgccgg
      121 aacagtccac aacataacag atacgctgga cgatgctttt ttggccgaaa aactccacct
      181 cattcggcac ggtcggctgt gccgctgatt cggatggcag ctgggattgc tgggccggca
      241 cagcagaatc ggcaggagac ggcatgggcg gcttttgctc ttcacagggt gcggcagcag
      301 tatggatcgg ctcgacctgt ggttttgggc gaggcccgga ttcctgaaca atatgctcaa
      361 tggcctgcag cgagatagca ggtttcacag ctgcctgcga aatgcggccg gacaacctaa
      421 cgctggtaaa aaatgccagc gccgctgaat gcacacccac cgaaacaatg agcgcccagt
      481 agcggatatt tctatggttg agtgttttcc ttatcatctc gtatttgaag ctgtcctgat
      541 aaatttgcag gatgtcctgt atgaacaggg ctgcagcggc ttttattcct caattaatac
      601 ttctgaaatg cccggataca acagattata cacttcaatt acttcgccgt tatgaagccg
      661 gatgcacccg cgggaagaac gcgttccaat ggtttccggc tctttggttc catgaatggc
      721 aaagcctgtc cggtttcggg tgttttcatc gatcccctcg atggcaatcc agcgcgaccc
      781 caacgggtaa tcggggtctg ttcccagata acgcttgccg gtgtcgggat ctgtccaggt
      841 gggcttgatg agcttgccgc ctttggctac tcgccagcgg ccggcaggcg tttcgtgctc
      901 ttctttgcct aatccgacac ggtaggtttt aatgtatttg ttctgcagat acaggtccat
      961 ggtgaaactt ttcttgtaaa ctactacatt aaagggaccg ttgatgacct tgattttttt
     1021 gcctgcctgc aagagttccg gacgcgaaat gccattgatt tccatcagaa tctcaaaggg
     1081 gactttatgg tctctggcga tatcctgaag caaatcgccg ggctgcacca agtactggcc
     1141 ggtgagttta tcatcgggaa atatctgcgg actgaagagc catttttgcg ccagtttgga
     1201 caattccagt tttacagccg aacgaatcac cggacttaac tgcatattca gtgcgtcatt
     1261 gagcaaatca cgggcgaaaa tgacctttcc ggcatctcga agctcagttg cctgcttgat
     1321 aatttccttg gctttttcac tggtttgtcc cgtatcggca ctggcggctg tcagaacatc
     1381 ctgcggaatc tgaacggttg ttaccgtttc tgccggcttg ggtacagccg ccggttcagc
     1441 cggcttgacc gattctgtcg gcactgctgt tccagagaca gcggttttgg ccggctcagc
     1501 gggtgtcggc gaaggaactg caggcgggac ggattctgtt ttcagcgcca ccgatggatt
     1561 aagaacacgc tgcggctcgg ccggcgcagc tgccggctta gccggcagaa cgttgtcgag
     1621 tgaaaccttc tgtacctcat ccggaacaga gtggggctgt ttgtttttcc attgctggat
     1681 gaaaattaca gccgccgcaa tcagaacaat cagtgctatg gcaatgatcc gaaaagagcg
     1741 gtctttctgc tgtttgcgtg taggataagg ccgatattct cgtgctgtca aaatagacct
     1801 ccttatcagt ttcgtttaca taggataatt ccgttttcag ttaccaccgc atctaccggc
     1861 acatcactgt cgtcatgggg tattgccggg catatttggt ggctgaaggc caaagcccag
     1921 cgggcagcag tgattttatt gctggtaaaa aaattgtcgt agtaagaccc gccccggccg
     1981 agccgattgc cttcggcatc aaaccccaat cccggcgtaa tcaccaagtc aatttcatca
     2041 aatgggacag gtatgccgcc gatgggattc cgcaagccgt gctcatcggt tttcagacct
     2101 gtctccaaag aggcaatctc tacgggtatc atatgacgct gctgccacga aattttcggc
     2161 accgctactg ttttgccgcg ctgccaagca gccaaaatca gcggcgtggt gtcgacttca
     2221 tgcggcagag acaaaaacgc cattaccacc gatgccttct ggaacacctc cgatcctatt
     2281 acataatcgc agattatttt gcttttggcg atgatctctt ccctgctcat ctgcatcagc
     2341 tgctgtttga gcttctgccg caatgcgctt ttatccatcc tgaattacct taattgtcag
     2401 tccgtttcgg attctgcctg ccgctgatac tgttgtacct gatttttgag tttttgtaaa
     2461 tattccgcga tgattttttc tctgccgata tttttgggcc gataataagg tttttccagt
     2521 ttctgaccaa gatagtcttg ttctacgtat cctgtctgtg attgatgggg atatttgtaa
     2581 tccaaaccgc gaccgagttt tttagctccg gcgtagtgag catcccgcaa atgaggcggc
     2641 acaggaatag tcctgcccga ctgcacatcc gatacggcct gccaaatggc gtttgcacag
     2701 gcattggatt tgggtgcaca agcaatataa atagccgcct gtgctagcgg aagctgcgcc
     2761 tcgggaaagc ctacaaattc ggcaatctgc aaggctgaag ccgctaaaat tgtcgcagta
     2821 ggatcggcat tgcccacatc ttctgctgcg caaactgcga ttcgccgggc gataaaccgc
     2881 aaatcttccc ctcctgcaat cattcgtgcc agccagtaca ctgtggcatc cggatccgag
     2941 ccccgcatcg atttctgcag agcgctggca agatcataat gtgtatcacc cgtgccgtca
     3001 taggcgatgg cctttttttg tacggactct ttggcaatct caacagtcag tttgataggg
     3061 gtactactgc gggcttgaga cattacagct acttccagag cggtcagggc tttccgcgca
     3121 tcgccgtcac agatttctgc cagaaattcc agcgcctctg ggtcggcgtc aacagccatt
     3181 ttgcccagcc ctttttcctt atcggccagt gcccggccaa gcagggtcaa gatggcatct
     3241 ttgctaagcg gctgaaattc aaatacagtg ctgcggctaa taagagggga attcacactg
     3301 aaatacgggt tttctgttgt tgctccaata agcgtgatga tgccgttttc aacatcatcc
     3361 agcaatacat cctgctgggc acgattaaaa cgatggattt catcgataaa aagcagagtc
     3421 ttgcgtttgc tggtcagcag ccggtcggta gcctgtgcta tgatatctcg tacatctttt
     3481 accgaagccg ccggtgcgct gagataatga aaggttgcct gaattgagcc ggcaacaatg
     3541 cgtgccaaag tcgttttgcc ggttcccggc gggccgaaga aaattaaact ggtaacattg
     3601 cctgcttcga gcattcgcct cagcagcttg tcgggaccgg caaaatgttc ttgtccaaca
     3661 aattcgtcca gtacagcagg acgcatccgt actgccagcg gtgcctgctt ttccagagct
     3721 ttccgctctc cttcgctgaa cagattatta ccgccttgag tcataatcag ccattatatg
     3781 tttttctttt acaaatgtcc aatccaaagg cattcggctg gatacacgat aaaccttata
     3841 ccatttatat atttttattt ggcaccgtaa ttggacggcc tgccgcagaa agaaaaaatt
     3901 gtggaatttt aggggcgatt tcggaaggcg gcagaagtgt tactgaaacg cttcaatagt
     3961 tccgccggcg acgatgtctt cgtcggtgtt atagagaacc agcttctgcc cgggacaggg
     4021 ggcaaactga ggtttttcaa atcggacggt cagatgaatg tcatcaaatt ctgtgatttg
     4081 gcattgatgc acggggccat atgagcgaag tttgccgaaa agtgtttgac cggtcttaat
     4141 ctgttcaggg atgaggatat tgacttcgcc ggctgttatg atcgtgtggc tgatttcttc
     4201 acgcgtaccc agagtgacgg tattgtgttc ggcgtcaatg cctgctacat agactggttt
     4261 tcctgctgca tagtgcagac cgcgccgctg gcccagagtg taatgggcaa tgcccttgtg
     4321 cgtaccgatg atgttgccgc ggatatccag caggttgccg ggtttatccc cttcaagcag
     4381 agcagcgcga taattgcctt cgccggcaaa acaaagttcc atactctcgg atttttccgc
     4441 tacgccgatg ccgatgtttc gggcgattgt gcgaacctcc tctttggtca tttgccccag
     4501 cgggagtaca aaatcagcca gccgccgccg cgggatgccg tataggaaat aactttgatc
     4561 ttttgcgtga tggtgtcccc gccgaaggcg cggtccatgg ggggtgtgca atacttgggc
     4621 atagtgtccg gtggcaagat gccgaatgcc gaattctttt tctaccaaat cccacataag
     4681 ggtaaatttc aaaagcctat tgcagtcagc gcagggattt ggagttcgcc ccctgcggta
     4741 ttcagtgcga aagcggtcga taatcaactc gttaaacgga gcagttacat ccacgaaata
     4801 gtggggcaac ttcagctggg cacacacata cgctgcttct gctccgcagc agccccgttt
     4861 atcagtgcgg cagactgtgg ggatgcgcat tgtaatgccc agcacgtccc agccggcctg
     4921 atgcagcaaa tgggctgtaa cggaactgtc aacaccgccg ctcatcagca cagctatctg
     4981 acgggacagg ctgttcttag gctcccacgg tgcaatatgc gaagaaatca attccaattt
     5041 atagtgattc atacttgtgt atccattaac tccagcactt tttccagtat ggtctgcaca
     5101 gtgccctctt gttcaatgtc aagccagcgg atgccgcgga atgttttgaa ccatgttcgc
     5161 tgggctttgg caaagcggcg ggtattgatt ttgatttgct cgacggcttc atccaagtct
     5221 atctgtcctt ttaaatgtgc aatcatctct gcatagccga tggcggccga ggcctgtttg
     5281 ctcagcggtt tcggctcggc cagcagggcc gcaacttcct cgcgcaatcc ctggtcaacc
     5341 attctcttta cgcgggcatt catccgtcgg ttttccgttt ccttcggtcg tcttaagccg
     5401 aatatctgcc agccttcggg cggatgcggc gtgttccatt gtttttggaa atgcgatatc
     5461 ggttttcctg tcagttcaaa cacctccagc gctcggataa tgcgtttttc atcattagga
     5521 tgtatccggc cggcggcctc ggcatccact gccgccagcc gtttgtgaag caattccagc
     5581 ccctcggcgg cggcctgctg cttcagcttt tgtcgaatat tttcatcagt tcccggtcct
     5641 tcaaacagtc catacagcag cgctttgata tacatcgctg ttccgccgac agctactacg
     5701 gttttttggc ggctctggat atccgcaaca gcctgttcgg ccagactaag aaatctatcc
     5761 acactgaacg aatcgctggg ttcaacaaca tcaatcaggt ggtacggaat aagccggcgt
     5821 ttttccagag gcggtttggc cgtaccgata tccattcgcc gatagacctt cattgaatcg
     5881 acgctgataa tttctccgcc cagcgcagag gccagctcgt gggccaatgc acctttaccc
     5941 gacgctgtca cccccagaat caggattttt tgtgttttgc ttgacataga gttcattgac
     6001 tgacaggcag gcattttact ggaaagccga cggtttggcg atagtaaaat tccggatgca
     6061 gctgcacttc tttggcaagc ctcttaagca cctgccgcac tctggacaag ggctatttag
     6121 gccataaata ggccagatag attccataac agatcagcat aaaaccgcct tccagacggg
     6181 acagaataaa gcggctccac atcagcggca ggcaaagtgc ggtgatagac gtcataaccc
     6241 acaggtcaac attggtgatg cccggggcag agtacggcat cagtaaggag gaaagtccta
     6301 aaattgctaa aatattgaag atattggaac caataatatt cccgacagat atatcgggct
     6361 gacgacgcac cgccgcaaca atcgaagtgg ccaattcggg cagactggtt ccggcactga
     6421 cgatggtcag gccgataatt gcttctgaca atcccagcag atgtgccagc cggacagagc
     6481 cgctgaccag catttttgaa ccggctacca gaagaaccaa ccctccagct attgcccctg
     6541 ctgctggaag tccgaacggt gtcagttgac tggttttctg gcggctgagc atctgttcga
     6601 cctcttgaag aacggtttga gggggctttt gacgggcctg ataaaaggca aaaacggtat
     6661 aggcaataat gcccgcaaac agaaaagccc cttccaatcg agacagatgc cggtcggcca
     6721 gcagaagaat gcaaagcaga gaaacaccaa tcagaatcgg aatatcgacc ttgacaagct
     6781 gtgctgtgat gcgaatcgga caaataacag ccgccagtcc cagaatcaag gcaatgttga
     6841 aaatattgga tccgataaca ttggcagcag cgatgtcgcc cgcgccatcc tgtgccgcct
     6901 gtatgctgac tacaagctcc ggcatactcg tgccgaatgc cacaatggtc agcccgatta
     6961 ccagcggtcc gactcccaga cgctgagcga tagcgacagc tcccttgacc agcagttcag
     7021 cccctgcata caagaataca agacctccga ttaaaagccc ggcaagaatc atttttttat
     7081 gtctcctgtt ttctctccat agagttgatt ttcggctgga gtgtttcaga gccgagaatt
     7141 cctcctgaca taattatttt gatgccctct tcgacagtca ttggacagcg gattacccgc
     7201 gattgttcaa tcacttccag aaaacccgtt gtgggatttg gggttgtggg gataaaaact
     7261 ttgtagcagg attggccttc cggattgatg atttggccgg tgataaatcc gaatgctccc
     7321 agaccggggg cgggaaattc aaccagaacc acttctttaa atgccgcctg ccgttcagga
     7381 ttcaggaaaa tccggactac atcccgtgat gagccgtaaa tggcattcag aaaaggaatg
     7441 ctaagaagca tctgatcaat tcgattcacc agccagcggc cggcaagatg tccggttgtc
     7501 aatcccagca ggtagatggc cgcaataaga attgccagcg atacatgtgt tttaatccag
     7561 actggaatat tgaggaatat ccaaagcagc acaggtgaca gcatgtccgt aataatgcct
     7621 acaagaaatt tcaggataac cgccgttata aacagcggca atactacgaa gaatcccgac
     7681 aaaatccttc gcttgagatg attggttatt gctgcagtca ttgtttatta tcctttattc
     7741 agttggcaat tgtatccaac atttttacat ttgaaaacat caactatata cacccgggct
     7801 tgaaatccat aggcacacgg cagcccgaag ggtatagacc cagcaggcac tgcatctgac
     7861 gcagaagagg cagggatttt tgccattggc ctatggcctg atgcagttgt gcgacatacg
     7921 gatgagagtt gacaggaatt cttttaataa tttcgatggc catctgattg taattctgca
     7981 gaagcaatgc cgtagcgtag ttggcctgat aaagcggcgg agtctcatca gggatgcaga
     8041 tatggttttg aaataccaaa tcccgcagca cagtgacggc ctgctcgata cggcctgcac
     8101 gcatcagaca tacgccgagg gcgtttttca tttcctgatt ctgagtgccg tttttatcaa
     8161 taaacagcag ggccttttcg gtttggtctg aacttaaaag ctctcgaatg tatccaattt
     8221 tgtcctgtaa agaaacatgt gttgatgaca taatatctcc tgataaccaa acagtttttt
     8281 gcaccttttt cgtaaacagc aatcagcaaa ttgccgtttc agcgaacaat acaatgttgg
     8341 gaatccggag agattacacg ttgagcatgc aattgagtgc taaaagggac gtgggattga
     8401 ttttgacgtg ccgaggtaac caataattgg aaatagttag gggtatattg gcttgaaaac
     8461 gtgcaaacac atagcaagca ttataagccc aaacttggtg agacaggttt tgactgcgcg
     8521 tctggaaact tgccgagtcg caataaactg gagagatata gtcgtcatta aaggcataag
     8581 gtgcggccgg aagtttatgg ttttttaaaa aagaatcctc caagccatcc gctagtcctt
     8641 gtgcaaacac cagaacggcc agcataccgc agaaaaaacg tattcgagat tgcttctgca
     8701 ttgtgagatt ttattgtaat tgctgaaagg ataacagatt cttctgctgg atacaagaga
     8761 aatcggtgtt gccagtactt tatcgaggga gcatatctgg taaaaaatca gttgtttttg
     8821 ctgttaatat cctttcagcg gaatataata atttgcagga atttctgaca ggatagacac
     8881 agatggatat tcaaacttca caaaaccaga acaatccaga aacatttaag gcatcgctgg
     8941 cagacaaagc aaacctgaca ggccaagtgc tggctgaact gctgggccag caggacagaa
     9001 tcccggtgcg tttgcgggag gcgatggact atatgcttca aagcgggggc aagcgcatcc
     9061 gggcagctct ggtgatgtgg acatgcgaaa ccgttgccag caagctcaac cgcgatgcac
     9121 agattgctgc ggctgcggtg gagatggtac atacctattc gctgattcat gacgatctgc
     9181 cggcgatgga taatgatgat atgcggcggg ggcggccaag ctgccataaa cagtttgatg
     9241 aggccacggc gattctggcc ggcgatgcgc tgctgacgct ggcctttgaa atcttgtcgg
     9301 aaaagataga ggatgccgag attgctgtgc ggatgattcg aacgctcagc agggcggcag
     9361 gaccgtcggg gatgatagcc ggacaggcgg ctgatatgca gtatgccaat accggcggca
     9421 cgttggcttc gctggaatac attcatacca acaaaacggg tgcgatgttt gctgcttcgg
     9481 cggccttggg ggcgattgct gcccgggcgt ccgaccgcca gattcagacg ctgctggaat
     9541 atggaatgaa aattggtctg ggttttcaga ttgctgacga cctgctggat attacagcca
     9601 ccagcaccca gctgggaaaa accgccggca aggatgccgc tcaggggaaa ttgacttatc
     9661 cggcattggt cggtacagaa aaatcacgtg aaatttttga aaagctgact tgtcaggcaa
     9721 tcgctgccgt tgaggatttc ggccctcagg ctgatatcct gcggcagctg gcaagggagc
     9781 tgctgaaccg gacacagtaa atgaatattc tgccgcatgc ggttctttca ggaatgttaa
     9841 gtattttgcc aatggctgtc ggcgggtgta aagtttgttc caaccggact ccggccgcac
     9901 ttcaatttgg tgttgcagcc gatgtgcagt atgcccagaa accgccagca atgggacggc
     9961 attatgcacg ctcgctggat gctctcaaac gctgtgtcga ggaattcaat cgccggtcgc
    10021 cggcgtttgt gattcagtta ggcgacctga ttgacggcgg ggaaaatgcc caagaagaca
    10081 tgcgcgaggc ggtttcggtc tatgatgctc tggtaatgcc gcattatcat ctgctgggca
    10141 atcatgattt ttcaggtctt ccgcgcagca gcacaatgtg tctgctgggt ctggagcagg
    10201 catggtatgc ctttgacgta cagggctggc gttttgtggt gctggatacg caggatttgg
    10261 ctgtagaggg cggctgggag aaatccagcc tgcgttatca gcagtcgctg gatatgctta
    10321 ttcagctcca agctgccggc gcagccaatg cacagactta caacggcggt cttggcagcc
    10381 aacagcttgt ctggctggat gcccaactga cagatgccga caggcagggc atgcccgtga
    10441 ttgtattcgg acacctgccc ctgatgccgt cggacgaaaa gcatattgcc tggaatgccg
    10501 aacaggtggt atcggtgttt gaaaagcatc gctgcgtgag ggcgtatttc tctgggcatc
    10561 gccacagcgg cggatacaca ctgcataacg ggattcatta tattactctt gaggcgatgg
    10621 ttgattcggc tgatacagac ggcgcatggg caattgtaaa acttgcgccg cagactatca
    10681 ttattgaagg caccggcgct gttgcttccc gaacgcttga actgccgccg cataaggcag
    10741 aacgccgccg gctggattaa gcgcagagta ttccataaag gtctttggga tagaccggct
    10801 cgataatgca ggttttcaca ccggcttcgt gcagatattt aacctgatgc ccctgaaagc
    10861 gggtcaggat acataccagc aagaccggag cggttttttc cagcagcatg acagcacggg
    10921 tcagcgtgat gtctttatca ggcgaggcat gaaaacagat aatagtatcg ttttcggcgg
    10981 gtgccttgag aaaaccctgc acatctgccg tctgaacagt caacccgctg tcgcaggcgg
    11041 ttttcaagaa tgcatccaag ttgtcaaaat cctctttgag gatcatcagt gtttttgccg
    11101 gagactgaaa cggagcaaac agatttgcaa catcaatttc cgacagcagc cgcttccatt
    11161 cctccttgcg gataccggcg gctttggcaa actggaggac aaactgcttt tcgacctcat
    11221 cgacccggcc gtcggccttg gccataacaa tcatcgcttt cagcgcagcg gcagctttgg
    11281 tatggtcatc cacaggccag cagccgaccg gatcttcgag gactgtctgc agcaggatcc
    11341 gccagttctt gactggtact gccagctctt gggcagctcg ggctagaaat gctttttcct
    11401 gcggctctat ctttccgtca cagcacatca gccgcagcag atttttcagg caattcaggt
    11461 tgtcctgcat tattgccata agccttcccg atacttttta tttcgtacaa atccccatca
    11521 taagcgctcg gtcttagccg tcaataaaaa ttattgtgat tggcattgta ccggattttt
    11581 caaaaaatta aacgtttgtt ttcggcttgc gtttaagaaa agatggggtt aatataaaaa
    11641 tcatcttgtt ctatggctgt ttaatttagg agacggcata tgaaaaagtg gctatttatc
    11701 gcatcagtta tcttaacggc ttcctttgtt ttggcagatg accagccatc ccgcccttcg
    11761 ccggacagtc cccgcccgca gagacaagaa gtgctgcggc cggcagccgc cgaacgactc
    11821 ggggctgtcc agcgtcagca gcaggaacgt ttagaccgcc tggaacagca gctcaatcat
    11881 ctgatcaaaa gactggaatc tcaggcagaa ccgattcccg ggccaaaagc tgttgtgccg
    11941 cctgtcaagg ctattaagcc ccagcgcggg ccgaggggac accggccttt ctggtgtgca
    12001 atgatggtct gctgtgctgt tgtacacatc ctttcagcag tctgggtttt tcaggatatt
    12061 cgtactcgcg gagccggttc aggagtctgg attgttattg ctctgcttgc cggtctgttt
    12121 ggcgtattgg tctatgcggt tgttcgctta ggcgatatac gcaagacctg atggctgcgg
    12181 attttaacca gcggttgctg acttcaggag gaagacagcc gctgcggaat gtgctgctcc
    12241 ctgaattgcc atttgcagcc ggcggaacga ttttttatgc agcatcctcg gccagcaggc
    12301 gttttataaa tagcaaatgg ggataatagc agattcggtt gtggatatat ttgctataat
    12361 accgcaagaa atggacgggt aaatcgatgc ttgctttgct caatataagt ggcttggaac
    12421 tcctttttgc ctgctgtgcg gttttcggac tgattttgtt tgtcatccgc ctgattttga
    12481 tgtttatcgg aatgggcagt gaaggtttag actcggcaga tgccggccat atcgatacag
    12541 gcgcaatgga atctcccggc caaggcggcg attttcatag cggcctgcac gacagcgatc
    12601 tgagctttaa gaccttgtct ttgcagggga taactgcctt tttcatgatg tttggtttgg
    12661 ttggctgggc ggtacttcgg cagggcaatt atgctgcctg gattccgatt ttatgcggtg
    12721 ctgccgccgg tgccgccact gtctgggtaa tgaaaaagat atttcaattg gccggttcgc
    12781 tgcaatctag cggcaccatg gatctgcaaa acgccgttgg acaggagggg acggtttatc
    12841 tgactattcg tcccggcaaa agcggcaagg tctaggtaac ttttcaggat cggcttagtg
    12901 ttttagaggc aattacacaa ggcagcgagg acatcaaaac cggtcagccg attcgtgtga
    12961 ttggcgtttc ggccggaaaa tgccctgagt ctgcagaata tccgcatcaa tgtgcccagc
    13021 acgtttaccg tcggtatcag caccgagccg gggattatga acaatgctgc tgagcgtctg
    13081 ctgcggctga atccgcatca gattgaggaa atggccaaag aaatcatttt tgggcagctt
    13141 cgtctgaccg tcgcttcact gacgattgag cagattaacc aggaccgcga aagttttctc
    13201 gaagccatcc gcaaaaatgt tgagccggaa ttgaataaaa tcgggttgtt tctgattaac
    13261 gtcaacatta ccgacatcac cgacagctcc aattatattg aaagtattgg caagaaagcc
    13321 gctgctgaag ccatcaatac cgccaaggta gatgtggcag aacaggagaa attcggcgct
    13381 atcggcaaag cacaggccgt ccgtgaacaa accatccgtg tggcagaaaa ctttgccgag
    13441 tctgaaaaag gccagaaaaa ggccgaggcc gacagggaga aagcaatccg cattgctgaa
    13501 aatattgctg ctgccgataa aggccaaaag aaggcggaag ccgacaagcg catttatgtt
    13561 cagggacagg aagcgctggc tgtaaccgga gaaaacaaag ccaaagcaga tattgcggag
    13621 gccgaagcac agcttgcgat caagcgtgcg gaagcactgc agaaaggtga ggtagcccgc
    13681 cggcaggcag aagtggaaat ccagaaagcg cagtatcttg ccgagcagca gcggcttaac
    13741 gccgaagtta ttgtccgtca ggaaatcgac aaacgcaagg tggagattga agccgaggcg
    13801 ctggccgaac gctaccgccg cgaagcccgc gggcaggccg atgccattct ggccaaatac
    13861 gaggccgagg caaagggtat ccggcaggtg ctcgaaagca aggccgccgg ctatgagtcg
    13921 ctggtcaaga gctgcaatgg tgacgccaaa gccgcttcga cattgttgat gattgaaaag
    13981 attgaagata ttgtcgcccg tcaggtggag gccatcaaga acctcaagat tgacaaaatt
    14041 accgtttggg acagcggctc agacaaaggt tcttcgacgg ccaattttct gtccagcatg
    14101 ataaaatccc tgccgccgct gcaggaaatt gcctccatgg ccggtgtcga gcttcccgag
    14161 tatttgggca agatggcaga caagcaaaac gcttcaggcg atgccaaacg gtaagggagc
    14221 gggatttccc tatatgtatc tgccctgttt gtcttttcaa aaaaagcggc atcccttatg
    14281 tgcttgcgca aatgggatgc cgctttgatt ttaaaacgcc cttggctgta ataaatagct
    14341 attgcatcgg acggatgacc atatcgttat cgtatttcat tgtaaaggtg taattgatga
    14401 cagtagcgtt tttcccgctg gtttcgggtt tcagtgtcag atcccatcgc aggatgccct
    14461 tatccttctg ggttcgcaaa tagtcggcgt cctgactcaa ggggtgcgat acggccgtca
    14521 gttcgataaa caaggccgga ttttctgtcc atggcagacg atccaacaga cggcaagcgg
    14581 ccggctcatt tttgtaattg ctgatttcga tgcggtactc ctgctgatct acacggttgc
    14641 cccagagagt ttcaatcttt ttatccttaa attcacgggc tacctgaatc tgagaatcca
    14701 cgccgaagcc ggcggtaaaa gtttgtccga tggtcaccag tttgatatcc gctttgccga
    14761 caaattcgcc gtctcggaat atatcgccgg ctcccggcag aaggatggtg ttgctggtgt
    14821 tggtaatgtc ggcaatcaga tatacataat cggtcagcag aggagctgct gcgaaagtga
    14881 acacagccgg acaatcgatg gccgcgatgg taacaagctg ttggtcggat ttgctgggca
    14941 aggtcagctt gccgggcagc ttatagatca cactgactcc ctcagcccgc tgtagggcaa
    15001 cagttctttc tttgatctgc tgaagaagcc gcttgtcggc ctcgaattca atcatctgat
    15061 tgtaaatagc aatctggttc aattccgcac tggccttgat gcctctggcg atagacgccc
    15121 gccgggactg ggccaattga gcaaaacgct cggtctggtc cccatacgaa taggcggatt
    15181 cctgctgctg ggtcggctgc tggaatggtt ctcccaagag tggttcaacg cgctgggata
    15241 cagctggctg gggtacagct gaaggagccg gcaatccgcg tttatctgcc ccagcgccgg
    15301 tcagggtaat ctgcatcgga tttaagaccg gcggggcggc aaccagcgcc ggtgcggcgg
    15361 tgcttaacgc caattcagtg ccaatccagt cttcgccgct gctttgatgg actacggcat
    15421 tgtaatcgat gtgcacattt tgatgagccg gattggcctt gaggttatac tgcggcgacc
    15481 agtcggcact gtcgactaag taactgagct gcacaatatc ggcagcgccg accgctggca
    15541 catgcacata aacaagtgcc tgtcgaatct gtcgtttata ttcggtgacc agtttgtctt
    15601 ttttttcgcg gactgccgcc aattgctttt ccaaatccag cttttgattt tccagaatga
    15661 ccagttcctt gtgaatttca ctgtttttag acgccataaa cacatttagg tcaataatgg
    15721 tcggagcgtt caggacgccg tgggtcatct cgacttttga ggtgtcggta atgaatttcc
    15781 actgctcacg gtagcggtcg agtcgatcgt tggcgttgtc catactgcgc accgtgtcgc
    15841 gcagctgacg ttctaaagta taaatctgat cttccagttc tttaacctct ttactgttgt
    15901 ctttttcaat ctctttgatg cgatagcgga ctgacagaac ctgaatgccg tcggaggcct
    15961 gcgcataaag ggtttcgggc agaatacgct cgggcaggtt ctcaaccagc agctccaaat
    16021 ccccgccggg gtggtctaat tgaatagtgc gggttaccag cgcctgtccg tgatacacaa
    16081 tgacgctgct gatgcggctt tcggccaact gcggctcagc aaaaagaggg cttgtcatca
    16141 ggaccattaa tacccctata aaaagttttg ttttcatttt ttttctcctt aaaataacgg
    16201 tgtctgtttt ttgtttttca gggcctctgc tctgccccaa ccgtaaacgc gtacccagtt
    16261 cccggtccgg cagccggctt tatccccaga aataatgcca agcgcaggtt agggttttta
    16321 gattcctttg gagataattc gactgtccat tcagctgttc cgacggtatt gaaccggtac
    16381 caccaccacg gccagctgca ccagctccac cagaaaggca gcccttcttc gaagacaagg
    16441 ccgtcatagc cgttgcccaa ctgtttgata attccatctg tatcagcgca gtccatattg
    16501 cccagcacgg aacgcctgac ctgaagcttg attgagcggt cgctgaagtt ggttagttcg
    16561 atagttcctt tcatctcgat tttcgaatag ttattgccat tccagttgac ggcgttaggg
    16621 atcataatgg tttgctcatc ggactttttg actccgatat tgaccgcggt ggtcagttcg
    16681 aggtcgccct tgttgccgac agctgtgtag gtcatcatcc cctgagcaag catccggccg
    16741 tctttgagga tcgccgccgg agctgtagta agggggtact cagaggtgtt gtccagccgg
    16801 attttatgga cggcattggg tttgtggaat aaccgggcaa gctgcaattg ctggtcgctg
    16861 ctgaagttgc ggcgcatttc cagaggcggg gcaaaagcga tatcaacggt gtaaatatct
    16921 tcgtaattca atgtcaattc ggcaacaggc agcaccatcc gctgtccctt gcgcaaagtg
    16981 aggtgttcga gcgaaaacac atacaaatct tctgtttttc cctcaaattc ctgccctaga
    17041 tctgtcgccc cgtcgccggg tgccggtgtt gcacgtcgtt cagcagcaga agaccgcatt
    17101 tggctcatta tggcattgga aagggcatag gccgtactgg catccggacg aaaatgactg
    17161 gacaatcggg cagcggtttc ctgaaatgaa atcggatctg gtgtatcttt gaatgcaaaa
    17221 ttgggcactc caataaccaa atgtgccgtt acatcgttca aatcagcaag ttcgtttatc
    17281 agtgttgcct gcagcttcaa gacagcatgt cctttcccgt caatttctac ccgataattg
    17341 ggaatccaac ggatgccgcg ctggacataa gccatgccga cggccgcttc agccgcaggt
    17401 gctcgtccgt tccagttaag atgcaacgtc atcgtttctt ttatttcttt ccggctgacg
    17461 gtgtcgctgg gattatccag aaacgtaacg gattgaatct gactgatggg aatcgcccga
    17521 atgccatcgg cggctttcag gaggacgatt ttttctcggg attgggattg cgggggctga
    17581 taagaataag catttaaggc tgggtcaatt tgagacgaag ggggaaaggc tttttcttcc
    17641 agtattcgca gtaccgttcc ttgccaaggt gcattgctgg tgatctcctt gataagggtg
    17701 tttttgccaa ggttgccctt gagcaggtct tccagcgtca aagctgtaca ttctgcatcc
    17761 aattcctctc ggctggaaac cacactggtc agcaccgctt tgggctcggc gctgtaagcc
    17821 cagaaagtac ccatcaccgg attgggcagc gaatccagcg ggatattgcc ctgactgtcc
    17881 gttttcattt tgccttcatg caaaatgtag gcgtgtccat ctttgaagat ggtaacttcc
    17941 ttgacaggca gtttgccgta aatctttttg gccggctgtt ccgggacagc atataataca
    18001 gccccggaca attcaatcag taccaaaatc cgcagggtca ggacaagacg aatgtgctta
    18061 ttcatttttt ttctccttaa aaatcagtta gcgttctatg attaataata cggcagaatg
    18121 ggaaaagaag gtgtaaaagg tctgtaaaat aggatccttt tggttttaca aaaaagttac
    18181 agttgcatag agcttttctt gcctataatg taatagatga tgaaaacgca tcagcacatt
    18241 ctgattgtag aggatgagcc gaagatacgg gatgggcttc gggacttttt ggagtttcac
    18301 ggatttgaag tcaccgcagc ggctgacggt ctggaagcgg agcggctggc agatgataag
    18361 cactttgacc tgattttgct ggatctgatg ctgccgcata tcagcggcga acagctttgc
    18421 cgcaaatggc gccagcaggg tctgacagcc ccggttatta tgctgaccgc caaaggacag
    18481 gagcacgagc gcatcgccgg actcgatatg ggagccgatg attacattac taagcccttc
    18541 tcgctcgaag agctgctggc acgcatccgg gcggtgcttc gccggaccga tcctgcccgc
    18601 tcggtcgggc agacgtttgc ctttgggcct tttacggtgg atacgcaggc gctttgtcta
    18661 atcgccggcg gccgcaggac gcccatcagt ccccggcaag cccagatgct ggtggtgttt
    18721 gccgcctgtc caaaccgcat tgtcagccgc cgggaattgt atgaaaaggt ctggcatgaa
    18781 tcactcgacg gcattgaaac ccgtacgatt gatatgcata ttgcccgtct gagggaactg
    18841 ctcaaagatg ccggggctca tgaggacctt atccagactg tccgaggcat cggttataaa
    18901 tatgaatgcg aacaataaaa agattctgaa cggacaaacc ggctgttttg agatattcga
    18961 atttgtctct tgcagatagt aaaagggacg gcagcataag gattggacaa gatttttgat
    19021 gttgaagcat ttcggtataa tttgtctggt tgtcgggatg gggctgctga ttctcagcgg
    19081 cttgggtctg tcgtcgctgg gaatgtacaa aaaagggctt gccgccgaac gccggcagga
    19141 gttcattgcg gttgccgaac aggttcgtta cgaaatcaag cagcagctgg acgctttttt
    19201 acaggccgaa cagggccggc cgtggacaga ttaccagtat gcttatgttc cggagtcgtt
    19261 taatcaggcc gctgctctgg ttagaagtcc gctggcagac ctgtttacaa acaatttggc
    19321 tgtcggctat ttccagctgg attctgccgg tcagattatc actccctaca gtccgccggg
    19381 gcaggaggtt cttatgccgc aggcggcagc ttattttcag aatatccgaa atcatctgct
    19441 cccttctctg cggttgggcc tgaatctgca gccctatcgc attgatccgt cggctccatc
    19501 cggagcgaat ggagcatatg gaaaactggc agccgaacga atcggcagtg tagcaatgac
    19561 accatcgccc gaccgcagtg aaactgcccc aacaaaaaaa gacatgccgg ccgtaaaatc
    19621 tcccgttcca gctgagcaaa aagcatctgc tgttaaagaa ccggatatga ggcgaggtgt
    19681 ctatgccgta tcaccgcttg aggatatcca tcagaaaaca caggttattc gccagagtcg
    19741 agcggcctat gaaatgaatg tggacaacac acaggttcta atgggccaag cccctgtatt
    19801 cggcagcgca cctctattgg atgaagccga gcagcagccg cattcccaaa ctcgcagacc
    19861 tgctgcggcc gaatcccaga gacaggtgcc gcaagaggca atcccgcctt cacttccgca
    19921 gtcccagaca gcaggggcat cgatgccagc aaatgcgttc agccggcagc aatctcaaga
    19981 cccgcggcag cgcccgatgg atttccagac aaccctatct gctgccgagc agatggagac
    20041 tgttcaggtg cgcatagagc cgtttgtgcc gattaccgtg ccggaacctg ccggccgcaa
    20101 tgggatattt gccggccaaa tatttctgct gcggcatgtt cagattgaag accgccactg
    20161 gattcagggt tttcagctta atgaagcgga actgctccgc cgggtgtctg acgccgttgc
    20221 gcggcttctg cggcggggaa tggactatga aatctcccgt caggagcggc ctgatgcggc
    20281 tcattcagcc atattggatt tcggttttgg ggaggtggtg ctgaacctgc tggaacagca
    20341 gcccgactgg attcagatgc aggcccggcg gctggccggc tggttttggg cgattttagc
    20401 agttgtatgg ctggctgttg cagcagcgat ggccgcctta tggacgaata tgcgtcagca
    20461 ggctcatctg tcccgcaaaa aagacgattt tatttctgct gtgtcgcatg agctgcggac
    20521 accgctgacc agcattcgaa tgtacaccga aatgcttgaa aataactgga ttaaaacaga
    20581 cgataaacgc cgccagtatt atacagcaat gcggcaggaa agcgaacggc ttagccggct
    20641 tattgaaaac gtgctggatt tttcgcggat tcagcgaaat aaaaaacaat atcattttgc
    20701 agtcggtgat gtcaatgatc caatccgtca ggccgcccag atgatgcgcc cgtgcatcga
    20761 gcaggccggc tttgtgctgg ttgaacagct ggatgagatt gctcccttta tgtttgaccg
    20821 cgacgctgtg atgcagatag ttgtcaatct gctggataat gccgttaaat atgcccgcac
    20881 agcggaagat aagcacatct ttctgcgcag ccggcaggtc cgggatcgga tttttataga
    20941 agtcgaggac cgcggccccg gcgtcccccg cagccagcag aaaaaaattt tcgatgcttt
    21001 ctaccgctgt gaggatgaat ctacccgcca gactgccgga accggtcttg ggctggcttt
    21061 agtcagacag tatgcccaag cccactgcgg aaatgttgaa gtagcggctg tgcagccctc
    21121 cggggcgctg ttccgcgtga tgctggacgt cagaacggtt aaatcgtctg gctgagccgt
    21181 tcaatggcct tgagcaggac tttgccgggg ggtttgatga tatgggcttc gcagccaagg
    21241 tcataacagt gtttggccag ttcctgatct tcgctggtag tcagcataat aaccgggatg
    21301 tctcgaagga cgggatcttt tttgattgt
//
LOCUS       DAOTBO010000041        21095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2052_length_21095_cov_5.551806, whole genome shotgun sequence.
ACCESSION   DAOTBO010000041 DAOTBO010000000
VERSION     DAOTBO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 21095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21095
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_21095_cov_5.551806"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLE88_06430"
     CDS             <1..470
                     /locus_tag="PLE88_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOM76136.1"
                     /translation="AHSQTIVEFGPGTGAFTKEILAEMPQNARLLAVEADPNLALLLR
                     KKFDKAVIAAGSAQKIQRIMKAHRFEAADCIISGLPWASFEPQLQDEILMEAEAVLKP
                     GGKFAAFGYVHALTLPGAKRFRQQLHRIFGPVQVSRVIWANVPPAVVYCCTKK"
     gene            complement(583..2067)
                     /gene="rpoN"
                     /locus_tag="PLE88_06435"
     CDS             complement(583..2067)
                     /gene="rpoN"
                     /locus_tag="PLE88_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOM76137.1"
                     /translation="MMKLSMTGQMRMEQKMKLAPRMIQSMEVLQLPMLALQEKIEAEL
                     NSNPVLEIAENREESAEASSDEFSEVSQERELVVREDSDYIDDFQRLENIKDVYGDYI
                     GNDESFSYRVSRLSASSGEEDKKMEALQNTAAQQLSLHDYLKEQWRMVEADTEIKAAG
                     DMIIDYIDEKGYLSVHLEQLHNKDKNGFGMEHLQKALELIQKLEPAGVGARDLRECLL
                     IQMRQFPDDMSLEMRVVQECWEELLENHLPLIAKKLNCSLEQVNRAIERMSKLDTSPG
                     LLVGRNENHPISADIIVEPDEHDGYTVRLVETKLPNLRVSEFYQKMAHDRRIDEQTRQ
                     FLQKNIRSAQWFMDAIRQRKHTLLRVANAIVKHQKDFFDKGKLYLRPLPMAAIAQEVG
                     VHIATVSRAVSGKYMQCPQGILPLRSFFSGGMEDACGQEHSWDAVKAKLQEIVDKEDK
                     SNPLSDDEIREKLMAEGMGNIARRTVAKYRKLLNIPTARLRKKY"
     gene            complement(2180..2782)
                     /gene="recR"
                     /locus_tag="PLE88_06440"
     CDS             complement(2180..2782)
                     /gene="recR"
                     /locus_tag="PLE88_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOM76138.1"
                     /translation="MNPAYTKTLNELVEQFAKLPGIGPKTAERLAFHILKSSTDEAMA
                     LSQAISNVKSRIQQCQICYNLAESEICGICSDSRRDKSVICVVEQPKDVISLEKTGLC
                     KWVYHVLNGHIAPLEGIEPEDLTIEALLNRVRQGSVKEVIMATNPNLEGDGTSLYIHS
                     LLRPFGIKITKLARGLPAGSTIEFSSGSILTDAILNRSEF"
     gene            complement(2786..4492)
                     /gene="dnaX"
                     /locus_tag="PLE88_06445"
     CDS             complement(2786..4492)
                     /gene="dnaX"
                     /locus_tag="PLE88_06445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOM76139.1"
                     /translation="MAYTVIARRYRSQTFDDVVGQDGVGKTLKNAIQTGRVAHAYLFC
                     GTRGVGKTTMARILAKSLNCLQSDSPTVTPCLKCDSCVSINAGEDIDVVEIDGASNRG
                     IDNIRELRQNALYRPARSRYKIYIIDEVHMLTTEAFNALLKILEEPPAHVKFIFATTE
                     PNKVLPTIQSRCQRFDFQSLSPEVIGRQLEKILKNENIEYEKDLVIYLSRLANGSMRD
                     SLSLLDQLISSGTQPLSTQQLAELIGIPDRQKVLNLLNAIACHDGAATLQSLDQLISS
                     GQSTRQICRTLIDFMRDLMVFLCAGPDNKILMLTQQEKQQLSQIAQEFDIASLIYNIT
                     ALEKLEWTIHNSESGRALLEASLLRLALSEHFIGLDQLRSAMGKDLPGGGSSGSVKKK
                     SLTDNSPAGTSPSLFENSTPPSVAPNPESPTSFWSALIKQCGDADASLPAYLQQARAI
                     SFENSTLCIQFPRTGQGQIAKNRCESKLDILGKFASQQAQTPVRLRIEFEPDSSSQQI
                     EKTMAQADSAPIVNRMKRQEILNDPAVQMVLQGLNATPVEIQKVDIAETDIENQLLEG
                     AE"
     gene            complement(4601..4699)
                     /gene="ffs"
                     /locus_tag="PLE88_06450"
     ncRNA           complement(4601..4699)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLE88_06450"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4891..5073
                     /locus_tag="PLE88_06455"
     CDS             4891..5073
                     /locus_tag="PLE88_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOM76140.1"
                     /translation="MIVHIEDNCTACGLCVDTCPEVFQMGSEHAEVIVKEIPAELEEL
                     VQQAADECPVEAIVVE"
     gene            5267..6901
                     /gene="pruA"
                     /locus_tag="PLE88_06460"
     CDS             5267..6901
                     /gene="pruA"
                     /locus_tag="PLE88_06460"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOM76141.1"
                     /translation="MQNTVSNIPKPLNETVYSYAPQTAPRTLLKQELHRLEQEQIEIP
                     LIIGGKEVKTGHLGRCIQPHCHSSCLAVFHMASEKEVLMAIEASQKAKICWQTMPYQQ
                     RASIFLKAAELLSSKYRYTLNAATMLNQSKNAYQAEIDSACELVDFWRFNCYFMQELY
                     KDQPLCSPQGTLNYVEYRPLEGFVFAVTPFNFTSIGGNLPTAPAMMGNTIVWKPASTA
                     VYSNYFIMKILQEAGLPDGVINFIPGSGSQIGKTVLASPLLAGLHFTGSSEVFNEMWT
                     FIGKHIRNYKTYARIVGETGGKDFLVAHISADPETLIAAVIRGAYEYQGQKCSALSRM
                     YLPKSLWRRVRDELVGQIKNIKVGPVQDFSNFMNAVIDKPAFDNAQRYIDHARCASDA
                     EILCGGGSNDAEGYFIEPTLILTSNPQYKSMVEEIFAPILTAYIYEDEDYEKMLELCD
                     QTSSYGLTGCVFAQDRYAICKAYETLRNAAGNFYINDKPTGAVVGQQPFGGSRGSGTN
                     DKAGSPLNLLRWISVRAVKENFLPVRNYRYPFMEQE"
     gene            complement(6932..7759)
                     /gene="proC"
                     /locus_tag="PLE88_06465"
     CDS             complement(6932..7759)
                     /gene="proC"
                     /locus_tag="PLE88_06465"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOM76142.1"
                     /translation="MNTIGLIGAGNIGEALIRGVLSSKLYLPDEVIASDVRPERLDYI
                     AKEYGIRTTQNNLELINYARVLIICVKPQQVARMLDEIAGKLNPDMLVISIAAGITTK
                     YLQNRLGNIQIIRAMTNTPVMVGEGATALYSATAKKEGVEHAIRLFSAVGKAVVVDRE
                     ELIDAVTAVSGSGPAYFFLLMEEMVTAAEELNIPADIAETLVFQTARGAGMLVNQAYA
                     RGESPAELRRKVTSPRGTTEAAIHVFLERDFGGTIFAAIKRARDRSLELSREAEQEN"
     gene            complement(7935..9575)
                     /locus_tag="PLE88_06470"
     CDS             complement(7935..9575)
                     /locus_tag="PLE88_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76143.1"
                     /translation="MRIRISHPALRDLFDQLRFSPLSQKKKQLAAAEQLGMIIDSQQE
                     YPYDFIVYRITGYRPRMDTPLPLISGKDLLLDLRVWIAQLSSELHIQADEQPEPLLTI
                     QQIARRFSVSAKTVRRWQKRGLTGKMYVFRDGRKRMGFLESAVQDFAQAHSEQIQRSS
                     RFSLLKSEEILQIKAMARELSATRRFRSRNELILEISSRLHRSRQTIRSVLNRWQKEE
                     KNPPCMPFTRGKIGPREAAQLYKLRQQGCSIPDLMQRFRRSRCSIYRIINQQRAKELL
                     ARRLEYIDSDEFHCGDAEQIILQMPLGDLLSGRQAETVLLSRQQEIELFRRYNYLKYL
                     AFQERSRLERRCIRSEQLGRIEKLLKEAEEVKNIIIEANMPLVASIAGRHLSSGQTLN
                     ELVSEGNMTLLAAVEKFDYRRGYRFSTYASWAIAKDFARLLPAESRRPDRAAGTDLAN
                     LPADLRLEQLSDAAAVEQAQRDLRKIIENNLDSREQYIILNHYALDAGAIPKKSKTLK
                     QIGEELGLSQERIRQIELQALQKLRQSLSPEQFDLLTK"
     gene            complement(9602..9982)
                     /locus_tag="PLE88_06475"
     CDS             complement(9602..9982)
                     /locus_tag="PLE88_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76144.1"
                     /translation="MKKTIEEIAHQDGRYDARALKFVFEGLAATIDKLRRQEDPEAAP
                     RHITGQELAWGLAEAAQKRWGRLAAMVLGYWGVHTTRDFGEIVYLMIDSGWMTSQETD
                     TIEDFDDVFGFAEVFEKQYRIEIR"
     gene            complement(10091..12097)
                     /gene="metG"
                     /locus_tag="PLE88_06480"
     CDS             complement(10091..12097)
                     /gene="metG"
                     /locus_tag="PLE88_06480"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOM76145.1"
                     /translation="MRKLLVTSALPYANGPIHIGHLVEYLQTDIWVRFQKMMGNECRY
                     FCADDTHGTPVMIRARSEGIAPEALIQRVHSEHYRDFTGFGIEFDNYYTTHSEENRQL
                     SERIYQQAFKIGAIVKKVIQQAYDEQEKMWLPDRYIKGTCPRCKAEEQYGDSCDVCGA
                     HYQTTELINPVSTISGKPPVLRESTHYFFRLGHFESALKELFAQGYVQATVQNKLDEW
                     FSAGLKDWDISRDGPYFGFKIPGEEDKYFYVWLDAPIGYMASCKNYCDRNGLDFEQVW
                     NGGEYEIYHFIGKDIMYFHALFWPALLLAAGLRTPDKLFVHGFLTVNGQKMSKSKGTF
                     IMAETYLRHLDPQYLRYYYASKLSGDVGDIDLNVEDFVNRVNADLVGKLANLASRSGP
                     MLTEKLGGRLGRLDTEGQMLFKELAAAKEEIVYSYENLNYAAAIRRITSLADICNKYV
                     EQHQPWAAIKSNPEYARTSLTSILNAVRVLTLYLKPVLPAFAAKIEQFLNVKPLTFAD
                     TETPLENHTIQKFIRLIERIEKEKVDAMIEESKPQAAAPAVTEPVSLDEPLAAECTIE
                     DFMKVDLRIARITKAAAVEGADKLLALELDLGALKKNVFAGIAKAYRPEELVGRLVVC
                     VANLKPRKMKFGISEGMICASGPGGTEVFLISPDTGAKPGQRVH"
     gene            complement(12103..13410)
                     /gene="ricT"
                     /locus_tag="PLE88_06485"
     CDS             complement(12103..13410)
                     /gene="ricT"
                     /locus_tag="PLE88_06485"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOM76146.1"
                     /translation="MSQVDSTKSKAAKNVKLMLVRYGRLGLLGWFEHHEPRIPRLNSH
                     VVIKTERGLELGEIVGIHSYRAGQFKSTPEQVEQYYYNRTKDYPIGEGGTFVRFATRE
                     DLHEQEHLEKNAIAEAKCCQRIADELGLNMRIIEAEHLFGGERIIFYFTSETRVDFRD
                     LVKRLAKEYQTRIELRQIGSRDEARLISDYETCGQQCCCSRFLKILEPVNMRMAKLQK
                     ATLDPSKISGHCGRLKCCLRYEDETYLELKQRLPHRGTAVQTPKGVGKIVDMQILTQL
                     VVVQTESGDRQAWPLEEIKILSSEEAEALTANKPAQSSNSQEPPFVYDNAGGSKKADE
                     ALEENSVLDDLNDEEGILPLSEEPQNGRLNQQSAPAVRQNGSSQEINRSKNRRRRSKN
                     KNRKPQNAPAGNAGRDLASQNGQTRSNPSDSSSNQPFSAPSGE"
     gene            complement(13477..14607)
                     /gene="holB"
                     /locus_tag="PLE88_06490"
     CDS             complement(13477..14607)
                     /gene="holB"
                     /locus_tag="PLE88_06490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOM76147.1"
                     /translation="MALKDIFCQDKAVEILQHAYASGRMAHAYLFSGDDGVGKRTTAR
                     AWAKMLLCENKRIISQNPYFADSCGQCHSCRTFEGGGHPDFQMITKELIQFTRESKNK
                     TTPIKMPIDVIREFLIEKIANRPMMGQYVVYVIDEAEKVNRESQNALLKTLEEPPKYC
                     VIILLCSQLDEMLPTTRSRCQLVRFGPVSEERIIQRLCSGGVSKTEAVFWSRFSQGSI
                     GQALAWSQLQIDDDAGLFAVKKELIEKIASLNLPDVLDIADWLSQNTKKIAAAWNSIS
                     KNVSISDITRRVEKGLIQMAVFALNDAMKLASGFSSSDLVNEDQLDSIHKLADKYDAE
                     TAAKQVEICYRLLNWVDSSVNEKLIYEQLLFNIANSDIMYSL"
     gene            complement(14607..15224)
                     /gene="tmk"
                     /locus_tag="PLE88_06495"
     CDS             complement(14607..15224)
                     /gene="tmk"
                     /locus_tag="PLE88_06495"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOM76148.1"
                     /translation="MSSMLKGKFIVLDGPDGCGKSTQVRLLAEHIAGAGIDVVSYRDP
                     GTTVVGEKIRAILLDPSHEGMGDNVEVLLYMAARAQLWLEHIGPDLAAGRCVLMDRWV
                     SSTCAYQGWAGGFGIERVIRIAADALTRVWADRTIILDVDLNTAKQRMCRQLDRMEQK
                     GDAYHAKVREGFLKLPDYDSNARIVNGRLSIENVHRDILNWIQQV"
     gene            complement(15221..16000)
                     /locus_tag="PLE88_06500"
     CDS             complement(15221..16000)
                     /locus_tag="PLE88_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76149.1"
                     /translation="MVQKAFDIVKARWPEAVLIVVLQAALMLLAEQLLSISQIEQGVK
                     PPQLSFWAGFLLGMGSILYVVLWQMIYLGFLKTAAAEGRQAQVPTELVRSGRPYFWRI
                     VFFQVLLGFVVFFINGILVGIGGAIFWKGRPLSQIPEWFGQLCGLAALLIVLKPMLLV
                     PARVLVYDESALEAFVKVRYYRIVHMDSLPQLALVGVGVIALTTLLGTTITPKTIWHV
                     VFMGLHHLIFSFVFLMPALTAVLWMQQQYDKEHYSQEENTE"
     gene            complement(16052..19525)
                     /locus_tag="PLE88_06505"
     CDS             complement(16052..19525)
                     /locus_tag="PLE88_06505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOM76150.1"
                     /translation="MADKGFTHLHLHTQYSLLDGAISAKKLFSRCSELGMSAAAITDH
                     GNMFGVVDFYIQAKEAGIKPILGMEAYIAPESRFDRQKGGGVKENAFHLLLLAENLTG
                     YRNLLKLSSIGFTEGFYYRPRIDMQVLAEMNDGLICTTACIAGQVPSALIRNDWEGAK
                     KAVSQYLDIFSRERFFIEIQEHDNEEYPDLRSKLIHLAKEMGVRLVATNDVHFLEKED
                     FEAHEVLCCINTGKKITDPDRLKYPPSAYLKSPQQMREMFSDVPEACDNTLLIAQRCC
                     VELDLSTRHAPVYRPENGSTPEEYLRKLVYEGAKKKYGRLTSEIEQRIERELTVISGK
                     GFASYFLIVWDFCNYAQKNNIPVGARGSAVGTVVGYCLGICDVDPIRYDLLFERFMDP
                     ERTEMPDIDIDICQVSRPAALEYVRQKYGQIAQIITFGTMKARAVIRDVCRVMNVPLS
                     EADRLAKLVPAELKMTLDKALEVEPDLKAAYEKDPATKRVIDIGRRLEGLARHASVHA
                     CGVVIADEPLTNFLPLYKQAGSDDLITQFEGPTVEKVGLLKMDFLGLKTLSVIQRAVD
                     LVESLHGKKIDIESIDIADQKVFRDIFGSGRTKGVFQFESGGMQDLLMKLKPDRIEDL
                     IAANALYRPGPMALIPDYIDRKHGAKWDVPHPIMREVLAETFGIMVYQEQVMRICNRL
                     GDIPLREAYALIKAIGKKKEKVIKAEKKRFVEGCLKKGLTESQAEEIFDLIERFAGYG
                     FNKSHATRYSFVAYQTAWLKCYYPVEFMAALLTYEMGSTDKVVEYIEECKQMGIEVLP
                     PDINESFVDFTVIYNTQRGPHPDKGVIRFGLAAVKGVGQKAVEQIIAAREKTGRFKSL
                     FHFCETVDLRAVNKQVLEALIKAGAFDSLGGSRAQMMAALEKAMQVGASMQADRVSGQ
                     MNFFGTGILNTTAQEDAKNLPDVPPWPEQQMLQYEKEVLGFYVTSNPLSRHAEMISVY
                     STANTSQLAQKGEGHSVIIGGMIAKIRYTVTKNGKSAGSKMAVFDLEDVQGKCEVVLF
                     PKTLEKYGALLKVDEIVFVRGTVDCRRELPNIVCDELYSMNEAAEKLAARVRIDLLTL
                     DVTEEKIRKIRQLCSTHRGKSPVNISVQTPGGYQIVALADKTLNVRADVDFIRKMETV
                     VGRGKVKLLRH"
     gene            19707..19790
                     /locus_tag="PLE88_06510"
     tRNA            19707..19790
                     /locus_tag="PLE88_06510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19741..19743,aa:Leu,seq:cag)
     gene            complement(19875..21056)
                     /locus_tag="PLE88_06515"
     CDS             complement(19875..21056)
                     /locus_tag="PLE88_06515"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOM76151.1"
                     /translation="MGDVTKVKTEAVCVPSTISVRKFHDSNANGIQDANEPDLEWLID
                     ITDPAGSPNTYLSPLTVLTTLTGTWQICEQYDSRWIQIALFVDSAAMPISLCAGVTVS
                     AGCDEVHEVVFGNIQAGSITACKFNDENVNGIRDIGELPVEGVRFVLSGSNIRGESVD
                     LIAYTGADGCVSFTGLLPGSYELCEVVPINWTATTDECRQITLEEGQDISFDFGNFCG
                     GFADFNTKGYWHNKNGLDETEDADIAYINTLAPWLACSTNTQPFGPINGKYCSGSNVP
                     AAKVGGAIIAPAGTPKAEQSIFLVYKNSIVKLQLAQQLYAFILNVRHRLTECGPDSWI
                     LLSDGTWKQASVLISEALAKWASGTNAERSAITKELDWLNNSMVAYRSCTPCEIVYPL
                     P"
BASE COUNT         5340 a         5370 c         4577 g         5808 t
ORIGIN      
        1 tagctcattc gcagacaatt gtagagtttg gaccggggac aggagcattt acaaaagaga
       61 tccttgcaga aatgccccag aatgcacgat tgctggcagt tgaagcggat cctaaccttg
      121 cgctgctgct tcgcaagaag tttgacaaag ctgttatcgc ggccggttct gcccagaaaa
      181 tccaacgcat tatgaaagct catcgatttg aagctgctga ctgcatcatt tcaggattac
      241 catgggccag ttttgaaccg cagcttcaag atgagatctt gatggaagct gaggcggtct
      301 taaaaccggg gggaaaattc gcagcatttg gatatgtaca tgccctgacg ctgccgggag
      361 caaaaagatt tcgccagcag cttcatcgca tttttggacc tgtgcaggtt tctcgtgtca
      421 tttgggccaa tgtcccccct gccgttgttt actgctgtac taaaaagtaa gttaatgggt
      481 cggactggta gttgccctaa ggcagcaaag gaacatcaac tcacgaaaat ttacattctt
      541 tcctgatcgg taaaatctga tccgagtgaa aagattaaaa tgtcagtatt tttttcgcag
      601 gcgagccgtt ggaatattta acagtttgcg gtatttggca actgttcgtc gggcgatatt
      661 gcccattcct tcggccataa gcttttcacg gatttcatca tcactcagcg gattggattt
      721 gtcttctttg tccacaattt cctgcagttt ggccttgacc gcatcccagc tgtgctcttg
      781 gccgcaggca tcttccattc ctccgctaaa gaaactgcga agcggcagga ttccttgagg
      841 acactgcata tatttacccg aaactgcacg ggaaaccgtg gcgatgtgca cgcctacttc
      901 ttgtgcaatt gcagccatcg gcaaaggcct gaggtagagc ttgcctttat caaagaaatc
      961 tttctggtgc ttaacaatgg catttgccac ccgcagaaga gtgtgtttgc gctggcgaat
     1021 ggcatccata aaccactggg ccgatcgtat attcttttgg agaaattgcc gtgtttgttc
     1081 atcaatgcgg cgatcgtgtg ccattttctg ataaaattcg ctaacgcgca gatttggcag
     1141 ctttgtttcg accaagcgaa ctgtatatcc atcgtgctca tccggttcta caattatatc
     1201 ggcggatatg ggatggtttt cgttccgccc cacaagaaga cccggagagg tatcgagctt
     1261 gctcatccgc tcaatagccc ggtttacctg ttccaaagaa cagtttaatt tttttgcaat
     1321 taatggaaga tgattttcca gcaattcttc ccagcattcc tgcaccacgc gcatttctaa
     1381 cgacatatca tcaggaaact gacgcatttg tatcagcagg cattcccgca aatcgcgggc
     1441 accgacaccg gcaggctcaa gtttttgaat caactccaag gccttctgca aatgctccat
     1501 tccaaatcca tttttgtcct tattgtgaag ctgctccaga tggacggata aatacccttt
     1561 ttcatctata taatcgataa tcatatcccc agcagctttt atctccgtgt cggcttcaac
     1621 catcctccac tgttccttca agtaatcatg gagcgacaat tgttgagctg ctgtattctg
     1681 gagggcttcc atcttcttat cctcttctcc gctggaagcc gataggcggg aaacccgata
     1741 tgaaaacgat tcatcgttgc cgatataatc gccataaacg tctttgatgt tttccagacg
     1801 ctggaagtca tcaatataat ccgaatcttc tcgtactact aattctcttt cctgtgatac
     1861 ttcagaaaac tcatcagatg aagcttcggc cgactcttca cggttttcgg ctatttctaa
     1921 aacaggattg ctattcagtt cggcctcgat tttttcctgc agcgccagca tcggaagctg
     1981 caaaacctcc atcgattgaa tcatccgcgg agccagtttc atcttctgct ccattcgcat
     2041 ctggcctgtc atagaaagtt tcatcatcgt ctccgcgtta gtttgagata aaaaagtata
     2101 tgggtgtagt cacacagttt tcgtcttctc caaattatac aaaaataagg cgaaaaatct
     2161 atgtcatgca tgaaaaattt cagaattcgc ttcggtttaa gatagcatct gtaagaattg
     2221 aaccgctgga aaattctatc gtggaaccgg ccggaagccc ccttgccagc tttgttattt
     2281 taattccaaa cggccgcaac agagagtgaa tatagagaga tgtgccatcg ccttcaaggt
     2341 tcggattagt tgccataatc acttctttaa cagaaccttg gcggactcga ttgagcagtg
     2401 cctcaatggt taaatcctct ggttcaatac cttcaagagg agctatatga ccatttagga
     2461 catggtaaac ccatttacag aggcctgttt tttccaagct gattacatct ttgggctgtt
     2521 ccaccacgca aatgacggat ttatctcgtc gggaatctga acaaatacca caaatctcac
     2581 tttcggcaag attatagcaa atttgacatt gttgaatcct gcttttaaca tttgaaatgg
     2641 cctgcgacaa tgccattgcc tcatccgtac ttgatttgag gatatgaaaa gccagccgtt
     2701 cagcggtttt tggaccaata cccggcagtt tggcaaactg ttcaacgagt tcattgagtg
     2761 tcttggtata agctggattc atcagctact ctgcaccttc caaaagctgg ttttcaatat
     2821 ctgtctctgc aatatctact ttttgtattt caactggtgt ggcatttagc ccctgaagca
     2881 ccatctgaac cgccggatcg tttaaaattt cctgacgttt catgcggtta actatggggg
     2941 cagaatctgc ttgggccatg gttttttcta tctgctggct tgaactgtcc ggctcaaatt
     3001 ctatacgcaa tcggacaggt gtttgtgcct gctgagaagc aaacttaccc agaatgtcca
     3061 atttgctttc acatctattt ttggctattt gcccctgccc cgtgcgtgga aattgaatac
     3121 aaagcgttga attttcaaaa gatatggcgc gggcctgctg caaataagct ggcagactgg
     3181 catctgcatc gccgcattgt ttgataaggg ctgaccagaa cgatgttggc gattcaggat
     3241 ttggtgcaac tgacggcggc gtagaatttt caaacagaga gggacttgtt cctgccgggg
     3301 aattatcggt cagagatttt ttttttacag aacctgagga gccgccgcca ggcaaatctt
     3361 tccccatcgc cgaacgcaac tggtcaagtc caatgaaatg ttcgctgagt gccagccgca
     3421 gcaaggaggc ttccagcagt gcccgaccgg attcgctgtt gtgaattgtc cattcaagtt
     3481 tttcaagagc ggttatgttg tatatcagtg acgcaatatc aaattcctgc gcgatttgac
     3541 taagctgttg tttttcttgc tgggtcagca tcaaaatttt gttgtccggg ccggcacaga
     3601 ggaagaccat caaatcacgc ataaaatcaa tcagtgtcct gcatatctgc cttgtacttt
     3661 gcccgctgct tataagctgg tcaagagact ggagagtggc cgctccatca tggcaggcta
     3721 tggcattcaa aaggttcaac accttctgcc tgtcgggaat gcctatcaat tcggcaagct
     3781 gctgagttga taagggctgc gttcctgagc tgataagctg atccagcagg cttagtgaat
     3841 cccgcatcga accgttggcc agtcgtgaca aatatataac gaggtctttt tcgtattcaa
     3901 tattttcatt cttcaggatt ttttcaagct gccggccaat aacctcaggg ctgaggcttt
     3961 gaaaatcgaa ccgctggcag cgtgactgga ttgtaggcag tactttattg ggttccgttg
     4021 tagcaaagat gaacttcaca tgagccggcg gctcttccaa tatcttcaga agtgcattga
     4081 atgcctccgt tgtcagcata tgcacttcat caatgatata aatcttatac cgcgaacgcg
     4141 ccggccggta taacgcattc tgacgaagct cacggatgtt gtcaataccg cggttgctgg
     4201 caccgtcaat ttccaccaca tcaatgtctt cccccgcatt gattgacaca caactgtcgc
     4261 atttgagaca tggagtaacc gtggggctgt ctgattggag gcaattaagt gatttcgcta
     4321 aaattcttgc catcgtggtt ttgccaacgc cgcgtgtgcc gcaaaaaaga taagcatgag
     4381 ccacacgccc cgtctggatg gcatttttga gcgtctttcc aactccatcc tgcccgacta
     4441 catcgtcaaa tgtctgtgat ctatagcgtc gtgctattac cgtataagcc atctaaaata
     4501 tccaaatatg cagtctcaac aattaaaaaa acggcttttt atacaataca tctgagaacg
     4561 gtaaaatgca atcgaaaaca ggaattcaca cgcccaaaaa ggtggtcggg tttaccacag
     4621 cacaacatca gaatcgctta tggctgctcg gttccccgcc tgaccaggtt cacgcccgat
     4681 gcttgcatgg gacccgaccg tcacaatgaa taacgatgtc tttcccagta cattttgaag
     4741 aagtaatgta ccaaaaaaaa acaaaatttc cactgaaaaa tcataaaaaa tatttcgcag
     4801 acattgatca tcagatatac taagaatgga ccatcaagag tccggctttc tggacaagag
     4861 agtaccgtag tttaatcgta ggagaaaaat atgattgtgc acatcgaaga caattgtact
     4921 gcctgcggtc tttgtgtaga tacctgcccc gaggttttcc aaatgggcag cgaacatgcc
     4981 gaggttatcg ttaaggaaat cccggcagaa ctggaggaac ttgtccagca agctgctgat
     5041 gaatgccctg tcgaggcgat tgtagtcgaa taattaccat tctcaacagt gaacgtcgcc
     5101 aaaacagaga tacaaacaga aggtgcattt taatcagccg tccctatcac atatgcaatt
     5161 cgaaaaataa gtattgtgtc agattgtctt gaagactctg cctgtttttc actataacaa
     5221 atgccatctg ttttgtgtgg atttttaata acgaaaggtt ttaatcgtgc agaatacggt
     5281 gagtaatatc cccaagccgc taaatgaaac ggtttattct tatgcccccc aaacagcccc
     5341 tcgaactcta cttaagcagg aactccaccg gcttgagcaa gagcaaatag aaatccctct
     5401 gattatcggc ggaaaagagg ttaagaccgg tcatctgggc cgctgtattc agcctcattg
     5461 ccatagctcc tgcctggcgg tttttcatat ggcaagcgaa aaggaagttc tcatggcaat
     5521 agaagcgtcc caaaaggcca aaatctgctg gcagaccatg ccttaccagc agcgggcatc
     5581 tatcttctta aaagcagctg aattgctgtc ttctaaatac cgctatacac tgaatgcggc
     5641 aacgatgctc aatcagagca aaaacgctta tcaggcggaa atcgacagtg cttgcgagct
     5701 ggtcgacttt tggcgattta attgttattt tatgcaggag ttatacaaag accagccgct
     5761 ctgttccccg caaggaacat taaactatgt cgaatatcgc cccttggaag gatttgtttt
     5821 tgcggttaca ccttttaatt tcacctccat tggaggcaat ctgccgacag cgccggctat
     5881 gatgggcaat acgatagtgt ggaaaccggc ttcaacggcc gtgtattcca actatttcat
     5941 tatgaaaatt cttcaggagg cgggtcttcc tgacggtgtc atcaatttta tacccggttc
     6001 cggcagtcag ataggcaaaa cagttctggc ttcgcccctt ttggctggtc tgcactttac
     6061 cggcagcagt gaagttttta atgagatgtg gacgttcatc ggcaaacaca ttcgaaatta
     6121 taaaacctat gctcgaattg tcggggaaac cggaggaaaa gattttcttg tggcccatat
     6181 ctccgccgat cccgaaacgc ttattgccgc cgttatccgc ggggcctatg aatatcaagg
     6241 gcagaaatgt tctgccctgt cgcggatgta tctgccgaag agtctatggc gaagggtacg
     6301 agatgagctg gtcgggcaga ttaaaaatat taaagtgggc cccgtgcagg atttcagcaa
     6361 ttttatgaat gcggttattg ataaaccggc ttttgataat gcccagcgct atatagacca
     6421 tgcccgctgc gcctccgacg cagaaatact ctgcgggggc ggcagcaatg atgcggaagg
     6481 ctattttatt gaaccgacac tcattcttac atcaaatcct cagtataaat caatggtgga
     6541 agaaattttt gctccgattc taacggccta tatttacgaa gatgaagatt atgaaaagat
     6601 gctggaatta tgcgaccaaa ccagttctta cggcttgaca ggttgtgttt ttgcacaaga
     6661 ccgatatgca atctgcaagg catatgaaac acttcgcaat gcggcgggta atttttacat
     6721 taatgacaaa cctactgggg ctgtagtcgg tcagcaaccc tttggaggtt cacgcggatc
     6781 ggggaccaat gataaggccg gttcaccgct taaccttctg cgatggatat ccgtacgggc
     6841 tgtaaaagaa aactttctgc ccgttcggaa ttatcgctac ccgtttatgg aacaggaata
     6901 atactcaata tcccatttac cggtgtaatt attaattttc ctgttcagct tcccgggata
     6961 attccaagct gcggtcgcgc gctcgtttta tagccgcaaa tatggttccg ccgaaatccc
     7021 gttcaagaaa gacatgaatg gctgcttctg ttgtgccgcg cggggatgtt actttccggc
     7081 gaagttctgc aggactttcg ccgcgggcat aggcctgatt taccagcatt ccggctccac
     7141 gggctgtttg aaatacaagt gtttcagcaa tatcggccgg tatattcaat tcttctgctg
     7201 cggtaaccat ttcttccata agcaggaaaa aataggccgg accagagccg ctgacagctg
     7261 ttactgcgtc tatcaattct tctcggtcca caacaactgc ttttcctacg gccgaaaaaa
     7321 gacggatggc gtgttctaca ccttcttttt tggctgttgc cgaatataac gcggttgctc
     7381 cttctccaac cattacaggg gtattggtca tagcacggat tatttggata ttgccgagcc
     7441 ggttttgaag gtatttcgtc gtaattccgg ctgcgatgga aatgaccagc atatcagggt
     7501 ttagcttgcc ggctatctca tccagcatac gggcaacctg ctggggcttt acacaaataa
     7561 tcagtacccg ggcataatta attaattcga gattattctg cgtggttcga ataccgtact
     7621 ctttggcaat ataatccagt cgttccggcc gaacatccga ggcaatgact tcatcgggca
     7681 gatacagctt ggacgacaaa actccacgga taagcgcttc acctatattc cctgccccga
     7741 tcaagccgat ggtattcatt gtttcccctt ttttctgtat ggaattttct tcaaagtttg
     7801 atgctgggta attttgacca ttgttttatc tgttgttgta ttcaactgct cactttcagt
     7861 aatgtcaaga aattctttta agaccattcc gattttcgtc tgcatataca gcgtgtgaaa
     7921 gtccattcca agcctcattt ggtcagcagg tcaaactgtt cggggctcaa actttgccgc
     7981 aatttctgta gcgcctgcag ttcaatctgt cgtatccgtt cctggcttaa tcccaattcc
     8041 tctccgatct gtttgagggt tttggatttt ttaggaatcg cgccggcatc gagcgcataa
     8101 tggttcagga taatgtactg ttctcgtgaa tcaagattat tttcaataat cttccgtaag
     8161 tcgcgctgtg cctgttcaac tgcggccgca tctgacagct gctccagccg aagatcggca
     8221 ggcaaatttg ccaagtctgt tcctgccgcc cgatccggac gcctgctttc tgccggcagc
     8281 aggcgggcaa aatctttggc aatcgcccag cttgcatacg tgctgaagcg atacccccgg
     8341 cggtaatcaa atttttcaac cgccgctagc aaagtcatat tcccttcgct gaccaattca
     8401 tttagtgttt gccccgaaga cagatgcctg ccggctatgc tggcaaccag cggcatattc
     8461 gcctcgataa tgatattttt gacttcttca gcttccttca gaagcttttc aattcggcct
     8521 aactgttctg agcggataca cctgcgttcc agccggctgc gctcctgaaa ggccagatat
     8581 ttgagataat tatagcggcg aaacaattct atttcctgct gtcggcttaa taaaacagtc
     8641 tcggcttgac gtccggagag caagtcgcct aatggcatct gcaaaataat ttgttcagca
     8701 tcaccgcaat gaaattcatc actgtcaatg tactctaaac ggcgggccag cagttccttg
     8761 gcacgctgct gattgataat ccggtaaata gaacaacggc tgcgtctgaa acgctgcatc
     8821 aggtcgggaa tggagcagcc ttgctgccta agcttatata actgagcggc ttctcgcggc
     8881 ccgatttttc cccgtgtaaa gggcatgcac ggcggatttt tctcttcttt ctgccatcga
     8941 tttagaaccg agcgtatcgt ttgacgcgac cggtgcagac gggaagagat ttcaagaata
     9001 agctcgttgc ggcttcgaaa acggcgggtt gcagataatt cccgagccat cgctttaatc
     9061 tgcagtattt cttcgctttt caaaagagag aatctgcttg atcgctgtat ttgttccgaa
     9121 tgggcttgag caaaatcctg cacagccgat tccaaaaaac ccatgcgctt gcggccgtct
     9181 ctaaatacat acattttgcc tgttaaaccc cgtttctgcc agcgccggac agttttggcc
     9241 gatacggaga atcggcgggc gatctgctgg attgtaagaa ggggttcggg ctgctcatcc
     9301 gcttggatat gaagttcgct gctgagctgc gcgatccata cccgcagatc taacagcagg
     9361 tccttgcctg aaataagcgg cagcggcgta tccatacggg gacgatagcc cgtaatccga
     9421 tagacaatga aatcataagg atattcctgc tgggagtcaa tgatcatacc cagctgttcg
     9481 gcggcagcaa gctgcttttt cttttgggac agaggcgaaa aacgaagctg gtcgaacaaa
     9541 tctctcagcg ctggatgact gatgcggatt ctcatacatc cttcacctct gcgatatctt
     9601 gttatcgtat ctcgatgcgg tactgttttt caaaaacttc ggcaaaaccg aagacatcgt
     9661 caaaatcctc gatagtatcg gtttcctgcg aggtcatcca gccggaatca atcatcagat
     9721 aaacaatttc accaaagtcc cgggtggtat gtacgcccca gtagccgaga accatcgccg
     9781 ccagccgtcc ccagcgtttc tgtgctgctt cagccaaccc ccaagccagt tcctgcccgg
     9841 taatatgacg cggagctgct tcaggatctt cctgccgacg gagcttgtcg atcgttgcgg
     9901 ccagcccctc aaagacaaac ttcagcgccc gggcatcata acggccatcc tgatgagcaa
     9961 tctcctctat tgtttttttc actgctgtct ttcctgctgt tctatgaaat aattagattt
    10021 tccagcaaaa atctttcaag caactgggcc ttacgaatgt ccaacagcac ctaaattggc
    10081 tgcccctata ttaatgaacc cgctggccgg gcttggcgcc ggtgtcagga ctgataagaa
    10141 agacttccgt cccacccggt ccggaagcac aaatcattcc ttcggatatc ccaaatttca
    10201 tcttgcgggg ttttaaattc gccacacaga ctaccagccg ccctaccaat tcttcgggcc
    10261 tataggcctt ggcaatgccg gcaaaaacat ttttctttaa ggctcccaaa tccagttcca
    10321 aagccagcag tttatcggct ccctcgaccg cagcagcttt ggtgatccgg gcaatccgca
    10381 aatccacctt cataaaatcc tctatcgtgc actctgccgc taagggctca tccagtgata
    10441 caggttctgt aacggccggc gcagctgcct gcggtttgct ttcttcaatc atagcatcca
    10501 ccttttcctt ttcaatgcgt tctatcaaac gaataaattt ttgaattgta tgattttcca
    10561 gcggtgtttc tgtatcggca aatgtcagcg gcttaacatt caaaaactgt tcgattttgg
    10621 ccgcaaaggc cggcaggacc ggctttaaat aaagggtcag caccctgaca gcattcagga
    10681 tagacgtcag agacgtacgg gcatactccg gattggattt gattgccgcc caaggctggt
    10741 gctgttcaac atacttgttg catatatctg ccagtgacgt aatcctgcga atcgctgctg
    10801 cataattcag gttctcatag ctatagacga tctcttcttt ggcagccgca agctccttaa
    10861 acagcatttg accctctgta tccagacggc cgagtctgcc gcccagtttt tccgtgagca
    10921 tcgggccgga gcggctggcc agattggcca gtttgcctac caaatcggca ttaacgcgat
    10981 tgacaaaatc ctcaacattt aggtcaatat ctcccacatc tccagacagt ttagatgcat
    11041 aataataccg cagatactgc ggatccaaat gccgcagata ggtttcggcc ataataaatg
    11101 ttcctttgct cttgctcatt ttttggccat tgaccgtcaa aaaaccatga acaaagagct
    11161 tatccggcgt ccgcaggccg gcagcaagga gcagggccgg ccaaaacagg gcatgaaagt
    11221 acattatgtc cttgccgatg aaatgataga tttcatattc cccgccgttc cagacctgct
    11281 caaaatccag tccgttccga tcacagtaat ttttgcacga ggccatataa ccaattggag
    11341 catccagcca gacgtaaaaa tacttatcct cctcccccgg aatcttaaag ccgaaatatg
    11401 gcccgtctcg cgagatatcc caatccttta gaccagcgct gaaccattca tccagtttat
    11461 tttgaaccgt ggcctggaca tatccctgtg caaataattc cttaagcgcc gattcaaaat
    11521 gaccaagccg aaagaaataa tgtgtgcttt ctcttaatac cggcggcttt ccgctgatgg
    11581 tgctgaccgg atttatcaat tcggttgtct gataatgcgc accgcagaca tcacaggaat
    11641 cgccgtattg ctcttctgct ttgcagcggg ggcaggttcc cttaatatag cgatccggca
    11701 gccacatttt ttcctgctca tcataggcct gctggataac ctttttgaca atggcaccaa
    11761 ttttaaaagc ttgctgatag atgcgctcgc tcagctgccg gttttcttcc gagtgggtgg
    11821 tgtaataatt atcaaactca attccaaaac cggtaaaatc ccgatagtgc tcggaatgaa
    11881 cacgctgtat cagcgcttcg ggagcaattc cttcgctgcg tgcccgaatc attaccggtg
    11941 taccatgggt atcatccgca cagaaataac ggcactcatt ccccatcatt ttttgaaagc
    12001 gcacccatat atccgtttgc agatattcta ctaaatgtcc aatatgaatt gggccgttcg
    12061 catatggcaa ggctgaagtt accagtaatt ttcgcataga tgctattctc cagagggggc
    12121 agaaaaaggt tgattggacg atgaatccga tggattggac cgggtttgtc cattctggct
    12181 ggcaaggtcc cggccggcgt tgccggccgg agcgttctgc ggttttctgt ttttattttt
    12241 tgacctgcgg cggcggtttt tactgcgatt aatctcctga gatgagccgt tttgccgcac
    12301 agccggagcg gactgctgat ttaggcggcc attttgcggt tcttcagaca gcggcagaat
    12361 cccttcctca tcgttcaaat catcaagtac cgaattctct tctaatgctt catctgcttt
    12421 tttacttccg ccggcattat catacacaaa aggcggctct tgagaattgc tgctctgtgc
    12481 cggcttgttt gccgtcagcg cttctgcctc ttctgaagaa agaatcttta tctcttccag
    12541 cggccatgct tgacggtctc ctgattcagt ctgcacaaca accaattgtg ttaatatctg
    12601 catatctaca atcttgccga cacctttggg agtttgaact gccgtaccgc gatgaggcaa
    12661 tcgctgcttt aattcgagat acgtttcatc ttcatagcga aggcagcatt tcaaccggcc
    12721 gcagtgcccg ctgatttttg acgggtccag cgtggctttt tggagtttag ccatccgcat
    12781 attaactggt tccagaatct ttaaaaaccg gctgcagcaa cattgctggc cgcaggtttc
    12841 ataatcgctg attaaacgcg cttcatcacg cgaaccgatt tgccgcagtt caattcgtgt
    12901 ctgatattcc ttggccaacc gcttcaccaa gtcacgaaaa tccacgcggg tttcgcttgt
    12961 aaaataaaaa atgatccgtt ctcctccaaa cagatgctcg gcttcaataa ttctcatatt
    13021 tagacctaac tcatctgcta tccgctggca gcatttcgct tctgcaattg catttttttc
    13081 aaggtgttcc tgctcatgca aatcctctcg tgtggcaaat cgaacaaatg tacccccttc
    13141 gcctatcgga tagtctttgg tgcgattata ataatactgc tcaacctgct cgggagtact
    13201 tttaaattgt ccagcacgat aactgtgaat gccgacaatt tcccccaatt ccaagccccg
    13261 ttctgtttta ataacaacat gcgagttcag acgcgggatg cgcggctcat gatgttcaaa
    13321 ccatccgagc aatccaagcc gtccatatcg aacgagcatc aatttgacat tttttgctgc
    13381 tttgcttttt gtactgtcaa cctgcgacat cttatataaa actcctcaaa gacgcagaga
    13441 ataaatccga ctaattatgc aaaatatcta attattttat aaactataca ttatatccga
    13501 atttgcaata ttaaacaaga gttgttcata gataagtttt tcgttaacgc ttgaatcaac
    13561 ccagttcagc agccgataac atatttcaac ctgtttagcg gccgtttctg catcatattt
    13621 gtcagctaat ttgtgaatac tgtcgagctg gtcttcatta accaagtctg aagaagagaa
    13681 accgctggcc agcttcatcg catcatttag agcaaaaacc gccatttgga tcagtccctt
    13741 ttccacacgc cgggtaatat cagatatgct gacattttta gaaatgctat tccaagccgc
    13801 tgcaattttt tttgtatttt gagacaacca atccgcaata tccagaacgt ccggcaaatt
    13861 tagtgatgca atcttttcaa taagctcttt tttgacagca aaaaggcccg catcatcgtc
    13921 aatctgcaac tgtgaccaag ccaatgcttg cccaatgctt ccttgcgaaa agcggctcca
    13981 gaagaccgcc tctgtcttgg aaacacctcc cgagcaaagc cgctgaataa tccgttcttc
    14041 tgagaccgga ccgaaacgta ccagctggca tcgagaccgg gttgtaggga gcatttcatc
    14101 caattgagaa cataataaaa ttatcacaca atatttcggc ggttcttcca gtgtcttgag
    14161 caaggcgttc tgactttcac gattgacttt ttctgcttcg tctataacat aaacaacata
    14221 ttgccccatc atggggcgat tggcaatttt ttcaataaga aattcacgaa tgacatcaat
    14281 tggcatcttt atcggtgttg ttttgttttt actctcccgg gtaaattgaa tcagctcttt
    14341 ggtgatcatt tgaaaatccg gatgtcctcc gccttcaaat gttctgcacg aatggcactg
    14401 gccgcaactg tctgcaaaat aaggattttg gctgataatc cgcttgtttt cacaaaggag
    14461 cattttagcc catgcccgtg ctgttgttct tttgccgaca ccatcatcgc cgctgaataa
    14521 gtatgcatgt gccattcggc ctgatgcata tgcatgctga aggatttcaa cagctttatc
    14581 ttgacaaaat atatctttca gagccattaa acctgttgta tccaatttaa gatatcccga
    14641 tgcacatttt caatagacag ccgaccgttg acaatccggg cattcgaatc atagtcagga
    14701 agcttcaaga atccttcccg cacctttgcg tggtaagcat ctcctttttg ttccatccgg
    14761 tccagctgcc ggcacatccg ctgtttagct gtgtttaaat caacatcaag gataatagtt
    14821 ctgtcggccc acacacgtgt caaggcatcg gcggcaattc gtatcacccg ctctatcccg
    14881 aatccacccg cccagccctg ataggcacac gtgctggata cccacctgtc catcagtaca
    14941 catcttccgg ctgctaaatc cggaccaata tgctcgagcc ataattgtgc ccttgctgcc
    15001 atataaagca atacttcaac attatccccc atcccttcat gtgacggatc cagcagaata
    15061 gcacgtattt tttctccgac tacagttgtg ccgggatccc gataggacac cacatctatt
    15121 cctgctccgg cgatatgctc tgccagaagg cgaacctgtg tactcttgcc gcagccgtcg
    15181 gggccatcca acactataaa tttacccttc agcatgctgc tcattcggta ttttcctctt
    15241 gtgaataatg ctctttgtca tactgctgct gcatccacaa tacagctgtc agtgcaggca
    15301 tcaaaaatac aaaagaaaag ataagatgat gcagacccat aaaaacaaca tgccaaattg
    15361 tctttggtgt tattgttgtg cccagcagtg tcgttaacgc gattacaccc acaccaacta
    15421 aggccagttg tggcagcgag tccatatgca cgattctata atatcgtact ttgacaaatg
    15481 cctctaatgc agattcgtca tataccagca cccgggccgg caccagcagc atcggcttaa
    15541 gcacaatcag cagtgctgca agcccgcaca attgaccaaa ccattccgga atctgtgaaa
    15601 gaggccgccc tttccagaaa atagcccctc cgatacccac caaaatccca ttgataaaaa
    15661 acacaacaaa tcccagcagc acctgaaaga aaacaattcg ccagaaataa ggccgtcccg
    15721 aacgcaccaa ttctgtcggc acttgtgcct gccggccttc agcggctgcg gtcttgagaa
    15781 atccaaggta tatcatctgc cacaatacta catataatat agagcccatt ccgagcagaa
    15841 aaccagccca gaaagacaat tgaggcggtt tcactccctg ttcaatctga gaaatcgaaa
    15901 gaagctgctc tgccagcaac atcagcgctg cctgcaggac cacaatcaga acggcttcgg
    15961 gccagcgggc tttgactata tcaaatgctt tttgtaccat ctttttacac aactaattta
    16021 gtgtacatcc tcaacaaatt tggacaagag tctaatgtcg caacagtttg acttttcccc
    16081 gccctaccac tgtctccatt ttacggataa agtccacatc ggcccggaca ttgagcgttt
    16141 tatctgccaa ggcaactatc tggtaaccgc cgggtgtctg cacgcttata tttacagggc
    16201 ttttgcctcg gtgagtagaa cacaattgac ggatttttct gattttttct tctgttacat
    16261 ccagcgtcag cagatcaatt cggacgcggg cagccagttt ctcagccgct tcattcatcg
    16321 aatacagctc atcgcaaaca atattcggca attccctgcg gcagtccacc gttccccgaa
    16381 caaatacaat ctcatctacc ttcagcaggg cgccgtattt ttccaatgtt ttcgggaaca
    16441 aaacaacttc gcactttccc tgcacatctt ccagatcaaa tacggccatt ttagatccgg
    16501 cgcttttgcc gtttttagtg accgtatagc ggattttggc tatcattccc ccgatgatta
    16561 ctgaatgtcc ctcgcctttc tgtgccaatt ggctggtgtt ggcagtcgag tagacactaa
    16621 tcatctcggc atggcggctg agtggattgc tggtaacata aaatccaagt acttcttttt
    16681 catactgcag catctgctgt tcgggccaag gcggcacatc ggggagattt tttgcatcct
    16741 cctgcgcggt tgtattcaat atccctgtgc caaaaaaatt catctgtccg ctgacccgat
    16801 cggcctgcat agatgctccg acctgcatgg ccttttccag tgccgccatc atctgcgcac
    16861 ggctgccgcc caagctgtca aacgccccgg cttttatcag tgcctcaaga acctgcttat
    16921 taaccgcccg aagatcaacc gtttcacaga aatgaaacaa gctcttaaaa cgccctgttt
    16981 tttctctggc cgcaataatc tgttcgactg ccttttggcc aacgccttta accgccgcca
    17041 agccaaaacg gataactcct ttgtctgggt gcgggccgcg ctgggtgttg taaatgaccg
    17101 taaaatcaac gaagctttca ttaatatccg gcggcagaac ttctattccc atctgcttgc
    17161 actcttcaat atactcaacc accttatcgg ttgagcccat ttcataggtc agaagagccg
    17221 ccataaattc caccggatag tagcatttca gccaagccgt ctgataagcc acaaatgaat
    17281 agcgggtcgc atggctttta ttgaatccgt agccggcaaa ccgctcaatc aggtcaaaaa
    17341 tttcctctgc ttgagattcc gtcaggcctt ttttaaggca tccttccaca aagcgttttt
    17401 tctcggcctt gatgaccttt tctttctttt tgccaatggc cttgatgagg gcataagcct
    17461 cccgcagggg aatatcaccc aagcggttgc agatgcgcat gacctgttcc tgatagacca
    17521 taataccaaa cgtttccgcc aaaacctcac gcataattgg atgaggcaca tcccacttcg
    17581 ccccgtgctt gcgatcaata taatcaggaa tcaaagccat tggcccgggc cgatacagcg
    17641 cattggctgc aatcaaatcc tcaatgcggt ccggcttgag cttcatcagt aaatcctgca
    17701 tcccgccgga ttcaaactga aaaacccctt tggttcgtcc ggacccgaag atgtcccgaa
    17761 agactttttg atctgcaata tcgatagact cgatatcaat ttttttcccg tgcagacttt
    17821 ctaccaaatc caccgcccgc tggatgacgc ttaacgtctt aagccccaga aaatccattt
    17881 tcagcaggcc caccttttca acggtagggc cttcaaactg cgtaatcaaa tcatcggaac
    17941 cagcttgttt ataaagcggc agaaaattcg tcagcggctc atcggcaata accacaccgc
    18001 aggcatgcac tgaggcatgg cgagctaaac cctccaatct tcgcccgata tcaatgactc
    18061 gctttgtagc cgggtctttc tcataggccg ccttcaggtc aggttcaact tctaacgctt
    18121 tatccagcgt cattttcagc tccgccggaa cgagttttgc cagtcggtct gcctctgaca
    18181 ggggcacatt catcacccgg caaacatccc gaatgacggc gcgggccttc atggttccga
    18241 acgttattat ttgtgcaatc tgaccatatt tctgccggac atattccaag gcggccggac
    18301 gggatacttg acagatatct atatcaatat caggcatctc cgtccgctcc ggatccataa
    18361 aacgctcaaa aagaaggtca taacggatgg gatccacatc gcatatccca aggcagtagc
    18421 caaccactgt tccgacggca ctgcctctgg cgccgactgg aatattgttt ttctgggcat
    18481 aattgcaaaa gtcccacaca ataagaaagt agctggcaaa tcctttgccg gatatgacag
    18541 ttaattccct ttcgatgcgc tgttcaatct cactggttag tctgccgtat ttcttttttg
    18601 ccccttcata taccagtttg cgcagatact cttccggcgt gctgccgttt tccggccgat
    18661 aaacaggggc atggcgggtg cttaaatcca gctcaacaca gcatctttgg gcaatcagaa
    18721 gcgtattatc acaggcttcc ggcacatcgg aaaacatctc ccgcatctgc tgagggcttt
    18781 tcaaataagc actgggggga tatttcagcc ggtcaggatc cgtaattttt ttccctgtgt
    18841 tgatacagca gagtacctca tgcgcctcga aatcctcttt ttcgagaaaa tggacatcgt
    18901 ttgtcgctac caaccgaaca cccatctctt ttgccagatg aatcagcttg gatcgcaggt
    18961 cagggtattc ttcgttgtca tgctcctgta tttcaataaa aaaacgttcc ctgctaaaaa
    19021 tatccagata ttggctgacc gccttttttg ctccctccca atcatttcga ataagtgcag
    19081 acggcacctg ccctgctatg caggccgttg tacaaattag gccatcattc atttcagcca
    19141 atacttgcat atcaattcgc gggcgatagt agaaaccttc tgtgaagccg atactgctga
    19201 gcttaaggag gttcctgtat cctgtgaggt tttcagccag cagcagcaaa tgaaaggcat
    19261 tttccttaac accaccacct ttttgtctgt cgaaacggct ttccggggca atataagcct
    19321 ccatccccaa tatgggcttg atgcccgcct ctttggcttg aatataaaaa tctactaccc
    19381 cgaacatatt tccatgatcg gtaatcgcgg ctgcactcat tcccaattcg ctgcatcggc
    19441 tgaacagttt tttagccgaa attgccccgt ccagaaggga atactgtgta tgtaaatgaa
    19501 gatgcgtaaa tcctttatct gccatcgaac cttcctgtag atttcaagtc tatcaactaa
    19561 aaccgtatca taataatgga atcgtaaaaa atcaaccata acatcggttg cgtatgttcg
    19621 aatcagtatg atcctgaata aatttcaaag tgccgaaaaa aggggttgac gaactcaaaa
    19681 aaaagtgtat atagtgcttt tttgctgcgg atgtggcgga attggcagac gcgcaggctt
    19741 caggtgcctg tgggggcaac cccgtggagg ttcgactcct ctcatccgca ttattatcgg
    19801 ctgtcaaaaa caaaaagccc gccttcttag aaaaaggcgg gcttttaatt atcctattcg
    19861 cttgttttct ttctttacgg caaaggataa acaatctcac agggagtgca gctcctgtat
    19921 gcaaccatcg agttgttcag ccagtccagc tcctttgtaa tggccgacct ttctgcgttt
    19981 gtgccggaag cccatttagc aagggcttca cttatcagca cggatgcctg cttccaggtg
    20041 ccgtcggaca gaagaatcca gctatcgggt ccgcattctg tcagccgatg acgcacattc
    20101 aaaataaatg catacagctg ctgtgccaac tgaagtttta caatgctgtt tttgtaaacc
    20161 agaaagatag attgttcggc cttgggggtc cctgctggag caatgattgc tcccccgacc
    20221 tttgcagcag gaacatttga accgctgcag tatttaccat tgatggggcc gaacggctgg
    20281 gtatttgtac tgcacgccag ccagggggca agggtattga tgtaggcaat atctgcatct
    20341 tccgtttcat caagtccatt cttgttatgc cagtatcctt tggtattgaa gtcggcaaat
    20401 ccgccgcaga aatttccaaa atcgaaggat atatcctgtc cttcctccaa ggttatctga
    20461 cggcattcat ctgtggtagc cgtccaattt atcggcacaa cttcacataa ctcatagctg
    20521 ccgggcagaa ggccggtgaa tgaaacgcag ccgtctgcgc ctgtataggc aattaaatct
    20581 acagattcgc ctctgatgtt ggatccagaa agaacaaaac ggacaccttc gaccggcaat
    20641 tcaccgatgt ccctgattcc attgacattc tcatcgttaa acttgcaggc ggtgatgctg
    20701 ccggcttgga tgttcccgaa cacaacttcg tgtacttcgt cgcatccggc tgatactgtt
    20761 acacctgcac aaagcgaaat cggcattgcg gcagaatcca caaacagcgc tatttgaatc
    20821 cagcggctgt catactgctc acagatttgc caagtacctg tcagcgttgt taataccgtc
    20881 agcggcgaca aataggtatt aggacttccg gctggatcgg taatatcaat cagccattca
    20941 agatctggtt cgttggcatc ttgaatgcca ttggcatttg aatcgtgaaa tttcctcacg
    21001 gatatggttg atggaacaca cacagcctct gttttaacct tggtaacatc ccccattttt
    21061 aggtccattg actaagttag gatttccctg taaag
//
LOCUS       DAOTBO010000042        20762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2099_length_20762_cov_5.754383, whole genome shotgun sequence.
ACCESSION   DAOTBO010000042 DAOTBO010000000
VERSION     DAOTBO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2099_length_20762_cov_5.754383"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLE88_06520"
     CDS             <1..1118
                     /locus_tag="PLE88_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76152.1"
                     /translation="DFDGDNRAEVALKTAPTDKDYRNPDDHLLPGRVMSGPEYCSILD
                     GMTGEELDRVEWPARGNPDDWGDAKNNRASRHLMGVAYLDGKRPSVLVLRGTYTLMRV
                     DAYNFENKKLKLLWQWSGDEENPKVRGQGMHGLHAADIDADGRDEIILGSAVLDDNGK
                     ILWNTGLGHPDACYVTDILPERPGLEIIYGIEPPCAQNGICLVEARTGKLLWGCQHPT
                     THAHSCGLFGDFDPANPGLEFYIGEKDRSAHWIYGMDGRLLSSENLGRLNPVPLYWDS
                     GSIKPYAYEGRIQYYHGQTIGRYEGMILAVADIVGDWREELITVVPGQLRIYTTAIPA
                     RDRRAALMQDALYRLDVAMVSMGYLYPPQLSYYLKQN"
     gene            1157..2116
                     /locus_tag="PLE88_06525"
     CDS             1157..2116
                     /locus_tag="PLE88_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOM76153.1"
                     /translation="MSNRVKVCIFIVLICANAGLGAEEPNQLETVWLFSYFKGNGEDG
                     LHLAYSTDGMVWRPLKNDESFLRPQVAGKLMRDPSICRGPDGLFHMVWTTGWWDKGIG
                     LAHSKDLMQWSRQEFLGVMEQEPAAKNCWAPEIFFDESTQTYLIFWATTVPGRFPQTD
                     NPRDDNNHRIYYITTKDFKTYTDTALFYDPGFNVIDAFIANAGQRYVMFFKNETKHPK
                     TEKNIRMAFADRASGPYGPPSEPITGKYWAEGPAAVKIGSRWIVYFDKYTERRYGAVV
                     SEDLVNWQDISGQVEFPGGARHGTVFPVSRDVAEKLLKTGTER"
     gene            complement(2122..2820)
                     /locus_tag="PLE88_06530"
     CDS             complement(2122..2820)
                     /locus_tag="PLE88_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOM76154.1"
                     /translation="MAKIKDKSGLVRRVWHTFCRLAIVSITQLYCRMRIWGRENIPSA
                     GPVLVLSSHQSFLDPILCQNWIWRPFYFVARDTLFKGFWGALIDSFYTIPIKQDQPDI
                     SAMKAIIDVLRQGNIVCLYPEGSRTEDGKIDRVKPGFSLLVRRTNACVVPMVIDGAFE
                     CWPRTAKYPKPHRVGVLYGQPIPADYIKEIGDEKFIDELNCILRTLHNTLRQKMGRPI
                     IDYALQNPLTASKS"
     gene            complement(2801..3490)
                     /gene="cmk"
                     /locus_tag="PLE88_06535"
     CDS             complement(2801..3490)
                     /gene="cmk"
                     /locus_tag="PLE88_06535"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOM76155.1"
                     /translation="MNRCIITIDGPAGVGKSTAARMLAGRLNAVFLDTGATYRAVTLA
                     AMQAGADLTDAKAVAGVMEQTQFEFIHQGDVLKVFINGRDCTQDIRLPEVTDKVHHIA
                     SQPQLRARLVKLQQDFASRFEAVVTEGRDQGTVVFPDAQHKFFLTADPYERTRRRYIE
                     LQTAGTPVDFDALAQQILLRDASDEKRSVGPLAPASDAVVIDTTCLDAQAVVEKMLSV
                     IQKKSYGKDKR"
     gene            complement(3487..4791)
                     /locus_tag="PLE88_06540"
     CDS             complement(3487..4791)
                     /locus_tag="PLE88_06540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM76156.1"
                     /translation="MNELEKKQDLIEEQIYRISTLVAGNFELQTVLDRLAEAAVKITN
                     TTACSIRLLDEEEGDLKMRSTYGLSEQYRNKGPVTKDDPVVKQAFQGQAVVIDDMRID
                     DRIIYREATLAEGLISQLTVAMVFKDQPIGVLRLYSPEPARFDRDAIRVARLVAAQCA
                     IAITNAKLYAQAIEGAKMAEQMRLAAVIQRRMIPQKAPCMCGLDIAAVYKPCYQIGGD
                     LYDFLQVGQKSLVVGISDVIGKGVPAAIMMSMFRGTLRAYADGGYGRHSMEEIIHKLN
                     RVACNECRDGEFITLFIARIDIDDNTLTYCNCGHEPALLIRGDKISELEEGGLVLGIL
                     PDAHYITRTIPFEQGDMLLMYTDGLIDAMNFDGQNWGKERLLAALPQCPRSTAEQFIG
                     SLLGYRRRFVGLANQTDDTSIVAIRRDNSVNTHGQPCSCSQV"
     gene            4957..6333
                     /locus_tag="PLE88_06545"
     CDS             4957..6333
                     /locus_tag="PLE88_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOM76157.1"
                     /translation="MEIDRKVGEQVVLFISVVKWAVLGMFVGGIVGCSTAVFIKTLNW
                     SISFTSQYPYYFLLMPAGFLASSLIVKLLAPQAEGHGTEKIIEAIHKQSGKISPAVVP
                     VKLVATIITIALGGSAGKEGPAAQIGAALASCFADLLRFDDNDRKKLVICGISAGFSS
                     VFGTPVGGAIFGVEVLFAGSMMYEVLLPSFIAGVTAYQVSSVLGLNYFQRPIEFIPVF
                     SGSFFIKVIAAGFFFGLVSLLFIEIMTVTHKAAKKIPLQLWIRPLLGGLICAILALVF
                     SRDYLGLGVETIESCLKGMPVVWYAFLMKVIFTSVTLAFGGSGGIVTPIFFIGAAAGN
                     TYGHFFGLDPAVFSSIGFVSLLAGCANTPISASIISIELFGPQLAPYAAAACIVSFLM
                     TGHRSIYSSQVLAIRKSASIDVQIGRELIAVTACAHPREKSLSAFLLRLIKKLKIVLK
                     GDFPQKQD"
     gene            6352..6891
                     /locus_tag="PLE88_06550"
     CDS             6352..6891
                     /locus_tag="PLE88_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOM76158.1"
                     /translation="MELFEAIKKRQSYRGGFTNQTVPREDLVKIVQAGLDAPSGKNCQ
                     TTTFVIADDPAVVNQIAQMHTTNKAMQQARAFIACIIDKNPAPSYEGYAFQVEDCAAS
                     VENMLLAITALGYGSVWIDGWLRLEGRNEAIGRLLGVPDDKIVRVLLPVGIPSEEYKR
                     PAKKAFNERAWFNRYGLSG"
     gene            complement(7391..7855)
                     /locus_tag="PLE88_06555"
     CDS             complement(7391..7855)
                     /locus_tag="PLE88_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76159.1"
                     /translation="MTLKYQHTQRGGLSFYLVLIVFTGLYAVLIIEAVNSRLQHDDKI
                     ALAICPLLGGYIILMLSGLTVTINDERIRIQFGPGVFWKTYPVNKIADCRPVRNCWWW
                     GWGIRWYIRGWLYNISGLDGVEITFKNGKKVRIGTDQPQSLADAINKAIQHT"
     gene            7950..8924
                     /locus_tag="PLE88_06560"
     CDS             7950..8924
                     /locus_tag="PLE88_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM76160.1"
                     /translation="MTHLFVKDIQPGRQIQDVYMVTQPVLRNTTKGDLYIAMYLSDKT
                     GKLNARMWQATEQIYQSIPSEGFIAIRGKSELYQGNLQIVVNEVQVVQPDQVRLSDYM
                     PRTEKNITEMYEQLKGILKTICNGDLKRLTEAFLNDQELMKQFCIAPAAMQMHHSYLG
                     GLLEHTLNMLQAAQALFGLYPKIQKDLVLVGIFLHDIAKTQELTYKMGFGYTDRGQLL
                     GHIIQGTQMITQKADDLKADGTPVDPTVLDSLLHIIISHHGQYEFGSPKLPATPEAFM
                     VTYLDNLDAKMNQTAALIETDAGDGGNWTSYQKSLETKLYRARVLNSD"
     gene            complement(8937..10205)
                     /gene="murA"
                     /locus_tag="PLE88_06565"
     CDS             complement(8937..10205)
                     /gene="murA"
                     /locus_tag="PLE88_06565"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOM76161.1"
                     /translation="MDIFEIDGPVRLAGSINVDGSKNSALPILAAALLAPGISTVSNA
                     PDLADIRSLETLLESLGAKVWRDGNGLHIDASVIDNPVGDYDIVRKMRASICILGPLL
                     ARCGRVKVSMPGGCAIGDRPVDIHLRGIKALGANVYLENGYIVAEAPAGGLIGTQIFL
                     GGPFGSTVLGTDNVMMAAATAKGTTVIESAACEPEVADLANFLNAMGAKISGIGSPRL
                     VIEGVKSLKPVTWSVIHDRIEAGTFMVAAAITRGQMKIQHCNLNHMMAVTDRLRHIGV
                     NVESCDDGCEVSFDGPIHPADITTQPYPGFPTDLQAQFMALLALGEGNSVITEKIYPD
                     RFMHVAELNRMAANLRKEGPSVIVAGVKKLIAAPVMASDLRASAALVLAGMAAEGMTT
                     IQRVYHIDRGYHRIEEKLNAVGAKIRRKSI"
     gene            10313..11188
                     /locus_tag="PLE88_06570"
     CDS             10313..11188
                     /locus_tag="PLE88_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOM76162.1"
                     /translation="MRDFWTILAGIAQERQAVLSEETEPFWDVFSISMTVLGLLVSAV
                     WMCQFGGFSALGKAPIRRHRMPAGLPMVLLGTWILMLAALSGLLRSPSDNLARSRFLY
                     LGMAVLEIILIAVMLTLAYRLFARRLKGFGFDFRTLLKDAGWAGLNLIGIYPFILAGL
                     WIILRIGRLVNPDFSLEVHESLQMLTDGSLTLKLLVAGFAVGVVPIFEEMLFRGFLQT
                     QIRTLTNRPWLAVVITSVIFAILHPTTHIAALFMLSCGLGYAYERSGSLFRPILMHFL
                     FNGISVAMTLLMPLA"
     gene            11345..11740
                     /locus_tag="PLE88_06575"
     CDS             11345..11740
                     /locus_tag="PLE88_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76163.1"
                     /translation="MSVRRQGLTLMELMVVILIAAILSAAAVPLLRGKIERSKWAEAN
                     AAAGVIRNAVKVYFLETGDTLTGNMADAAVLNALHLEAADITGTYFTASDYVIDNVDT
                     DGTAVITVTASKTNAPSGSKKLTASGSFE"
     gene            11920..12285
                     /locus_tag="PLE88_06580"
     CDS             11920..12285
                     /locus_tag="PLE88_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM76164.1"
                     /translation="MDKANPNLRVEFHGLIVVATLTDEKILEESQLKGLEGSFMPLIA
                     ETPAIQLIVDFSNVQFLTSSVLGLLIRISKKIYEKQGKLRLCSINPKILEIFRITRLD
                     KIFEIFPNVDEAMIGLKRS"
     gene            12387..12842
                     /locus_tag="PLE88_06585"
     CDS             12387..12842
                     /locus_tag="PLE88_06585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM76165.1"
                     /translation="MAEVSKILHQSWEIHSTESEAKRIIQTLLSTLQEHAFSEDMIFA
                     IHLSLEEAFVNAIKHGNHSDPEKMITVHCLITPEKFDISIADEGFGFDPQGIPDPRSN
                     GNLYKCTGRGILLIQSYMDVVEYNDRGNCIHMVKYRNSQKPVTPKTTQS"
     gene            12991..13752
                     /locus_tag="PLE88_06590"
     CDS             12991..13752
                     /locus_tag="PLE88_06590"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOM76166.1"
                     /translation="MGKIEKFFMAAVICIAAAECLPAVQTEPNSPAELNIGFGIAAYN
                     SVYEGDDDKVLAVPMLFYESEKIFFRGRMGGYRFFGDQTFSVALIGQWRVDGYDDNDS
                     RHLRGMKDRKMTLDGGLSATYVDGWGATTVSLVTDLLGRHNGQEFSISYGKRFSSGQW
                     SFIPSAGVLYKSADLTDYYYGVKAGEAAAQRPAYSADDVWDPFVAVQTSVKISRRWSF
                     LAALRYEWLGSEITDSPIVQSHYQTLLMAGMLCRF"
     gene            14007..16211
                     /locus_tag="PLE88_06595"
     CDS             14007..16211
                     /locus_tag="PLE88_06595"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOM76167.1"
                     /translation="MKKWLFGGMIVFVIVWNGCAASPQAVQQHEALQPQQIRAVMASV
                     ADWQLAHPSERPTADWTHGALFAGLTAWANMAGSDAYFKSLLAFGEKNNWQPYRRPYH
                     ADDHCVGQMYIDLYKLYKDPKMIAPIQQRFDFILANPPASELKMVREGNQDRWNWCDA
                     LFMAPPVWVKLAGVTGQQKYLDYMNKEWWATTDYLYDKKEHLYYRDSRYFDQREANGQ
                     KIFWSRGNGWVFAGLARVLEEMPEDYSDRERYVELYQQMAAKLISIQPRDGLWRASLL
                     DPQSYPTPESSGSGFFCYGLAWGINHGYLDEKTYLPAVVKAWQALVGCVHPDGKLGYV
                     QPIGKDPKQVSADLTDIYGVGAFLLAGSEVYKISVRNGAAAVKLNIANPAVIFRDNET
                     VELDWSRLQKAVRGLSKENAAVFDFKTNRLLVTQFIDEGKSTKLLFQSSFAPGEKKYF
                     WVMPQPPALEKAVSEYTTYCRFIPERKDDFGWENDKVAFRMYGPALEYETITSGIDAW
                     GKCVPTLVIDKFIKNYVEKQIPYHQDHGEGGDFYKVGPTLGCGGLAPFVDGKVILPRN
                     FVQWKVLANGPIRSLFELSYNPWQAGSASVSEVKRISIDLGSNMNRIECTYTSPNAQT
                     LPLAAGIILRETSDRKWTSDQTIAYWLPTDFITGNMGCGVVFGADYKTNTIEADSHLL
                     LTLSQKVGSPVVYYAGSCWDKNEEFSDFEKWQQYLVNFKKRIDNPLVIKMNK"
     gene            16240..17070
                     /gene="kduI"
                     /locus_tag="PLE88_06600"
     CDS             16240..17070
                     /gene="kduI"
                     /locus_tag="PLE88_06600"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOM76168.1"
                     /translation="MEVRYAPDPVRFSRMTTQEIRDNFLIENLFTPNRIDLVYSDVDR
                     AIVGSAVPKEKTLELSSAEELKAEFFCQRRELGVLNIGQSGTVAVDGRTYALGSKDCL
                     YIGRGSKTISFASSDAGRPAKFYLLSYPAHKEYPAAHIKIERANPIRLGSIETANKRT
                     IYQFIHPKGVQSCQLVMGFTVLEPGCVWNTMPAHTHERRMEVYMYFDMPDNVRVFHLM
                     GRPNETRHIVVKSEQAVIAPSWSIHSGAGTGAYSFCWGMGGENQAFDDMDHLTMDDIK
                     "
     gene            17088..17849
                     /gene="kduD"
                     /locus_tag="PLE88_06605"
     CDS             17088..17849
                     /gene="kduD"
                     /locus_tag="PLE88_06605"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: protein motif:HMM:TIGR01832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HOM76169.1"
                     /translation="MILDQFKLDGKKAIVTGGARGLGQAIAVGLAEAGADIALADVLD
                     MSETKGQIEKLGRRCIAITADLSRKESADIIVSQTVKQLGGIDILFNNAGIIRRAPFT
                     EFSEKDWDDVMNINIRGLFFLSQAAVKVMIQQGRGGKIINTASMLSFQGGILVPSYTA
                     SKSAVMGLTRLMACELAKYNIQTNAIAPGYMATDNTKPLRDDPVRNKAILDRIPAGRW
                     GTPEDLKGVAVFLASAASDYMNGYTVAIDGGWLAR"
     gene            18124..19833
                     /locus_tag="PLE88_06610"
     CDS             18124..19833
                     /locus_tag="PLE88_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOM76170.1"
                     /translation="MRKKLGQLLAEKKYISQAQLASALQRQQKDRHKLGQILQEEGLI
                     NTRQLTEALSEQAGIEKLDDVEKIPIDKDIISLVPAEVVSQYRLLPISRQNGRLTVAM
                     VDPFDAEAIANLRLLTGLSIRRCYCSPADLEKATRRHYGSNVARMVNDLAPAEKKAAQ
                     TVETIDTAQMTTARLHELAREPSLVNLVNLILLEAIEARASDIHIEPFETEIKIKYRI
                     DGMLVEKSSSPKRLHPAIVSRIKIMAAMNIAERFVPQDGHIEFSGPKGKIDIRVSTVP
                     TIFGEAVTMRLLDRSAALIQLSDLGMSPHTLAGFSQCLTKPHGIVLVTGPTGSGKTTT
                     LYAALNKIYDPSLKIITIEDPVEYQLDGVVQMPVNPRRGLTFSRGLRHILRQDPNIIM
                     VGEIRDKETADIAIRAALTGHLVFSTLHTNDAAGAVTRLIDMGVEPFLLASSLEGVLA
                     QRLVRKICPFCRVPYHPAESLLNSLNNSVRIPLDAALYHGTGCDECNHTGLSGRIGIF
                     ELLRITDTLRKLIAERPTTEQILAAAPSDHVSMRHDGIEKILSGLTTPEEVLRVTHTV
                     EEE"
     gene            19893..>20762
                     /locus_tag="PLE88_06615"
     CDS             19893..>20762
                     /locus_tag="PLE88_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM76171.1"
                     /translation="MFQYKGIDAAGRPIAGSIEAVDRKAAIAELAGQGCFVRQIQESA
                     LDAAGTIGTIQSGISRSGIKAKDILAMTAQLATALEAGLPMFSALEILKSQQKKAAML
                     NLLNELTGAVSSGQSLSEALGRHPNLFSSLYLSMVRVGETGGILEKTMQQLVGLMSRE
                     EKIRSNLISASAYPLFVLSAGLISMVIILVWVLPRIIRTIGIDPSMLPLPTRMLMGFS
                     HFLLAFGWLAALGIGFAVYCFIRWKRTPGGRMTWDSFKLKLPLFGNVVRSLAVSEPTL
                     RAKRPTASDRTTLP"
BASE COUNT         5250 a         4712 c         5469 g         5331 t
ORIGIN      
        1 atgattttga tggggacaac agagcagagg tggcgctgaa aacggccccc acagataagg
       61 attaccgcaa tcctgacgat catcttctgc ccggacgagt gatgtcgggg ccggaatact
      121 gttctattct ggatggtatg acgggagaag aattggatcg ggtagagtgg ccggccagag
      181 gcaatcctga tgactggggg gatgcgaaaa acaaccgggc ttcacggcat ctgatgggag
      241 ttgcatatct tgatgggaaa cggcccagcg tgcttgtgct gcgggggact tataccctga
      301 tgcgggtgga tgcttacaat tttgaaaata agaaacttaa actcctgtgg caatggagcg
      361 gcgatgagga aaatccgaag gtccgggggc agggaatgca cggccttcac gcggcggaca
      421 tagatgcaga cggccgggat gaaatcatcc tcggctctgc agtgctggat gataacggca
      481 agatactctg gaataccggg ctggggcatc cggatgcatg ctatgtaaca gatattctgc
      541 cagagcggcc gggtttggag attatttacg gcattgagcc accatgcgcc caaaacggca
      601 tctgcctggt cgaagcccgg acaggaaagc tcctttgggg ctgccagcat ccgacaacgc
      661 acgcacacag ctgtggactg tttggagatt ttgacccggc caatccggga ttggagtttt
      721 atattggcga gaaagaccgc tctgctcact ggatatacgg aatggatggt cgtctgctca
      781 gcagcgaaaa cttggggcgg ctcaatcctg tgccgctgta ttgggattcc ggaagcatta
      841 agccgtatgc gtatgaggga cggattcagt actatcatgg acagaccatc ggccgctatg
      901 aaggaatgat tctggcggta gcagatatcg tcggcgactg gcgtgaagaa ctgattacag
      961 ttgtgccggg gcagctgcga atttatacca cagctattcc cgcccgcgac agacgtgctg
     1021 ctctgatgca ggatgcgctt tatcgattgg atgtggcaat ggtgtcgatg ggctatcttt
     1081 atccccccca gctgagttat tatttaaagc agaattaata catttaggaa atgcagacac
     1141 aacaggagtg tccaaaatga gcaaccgcgt gaaagtttgt atttttattg tcttgatatg
     1201 tgccaatgcc ggccttgggg cagaagaacc gaatcaattg gaaaccgtat ggctgtttag
     1261 ctatttcaaa ggcaacggcg aagatggcct ccatttggct tacagtacag acggaatggt
     1321 ctggagaccg ctgaaaaatg atgaatcgtt tctgcgcccg caggttgcag gcaagctgat
     1381 gcgggatccg agcatctgcc gcggtccgga tggcttattt catatggtct ggacgactgg
     1441 atggtgggat aaaggcatcg gcttggctca ttccaaagac ttgatgcaat ggtcccggca
     1501 ggagttcttg ggcgttatgg aacaggaacc agcggccaaa aactgctggg caccggaaat
     1561 cttttttgat gaatcgacac agacttatct gattttctgg gcaacgactg tcccggggcg
     1621 gttcccgcaa acggacaatc cccgagatga caacaaccac cgcatttatt atatcacaac
     1681 caaagatttt aagacctaca ccgatacagc gctgttttat gatccgggat tcaacgtgat
     1741 tgatgcgttt attgccaacg ccggccagcg gtatgtgatg ttttttaaaa atgaaaccaa
     1801 acacccgaag accgagaaaa atatccggat ggcctttgcc gacagggcat ccggtcccta
     1861 tgggccgccg tcagaaccga taaccggcaa gtactgggca gaaggtcctg cggcggtcaa
     1921 aatcggcagc cggtggattg tctattttga taaatatact gagcgtcgct atggggccgt
     1981 tgtatccgag gatttggtta attggcagga tatctccgga caggtggaat ttcccggcgg
     2041 agccagacat ggtactgtat ttcccgtcag ccgggatgtg gcggaaaaat tgctgaagac
     2101 aggaacggag agatagaaat tctacgattt tgaagccgtc agggggtttt gcaaagcata
     2161 atcaatgatg ggtcgcccca tcttctgccg caatgtattg tgcagggtgc gcaggatgca
     2221 gtttaattca tcgataaact tttcatcgcc tatttcttta atataatctg ccggaatagg
     2281 ttgaccatat aaaacaccga cgcggtgggg tttggggtat ttggcagtcc gcggccagca
     2341 ttcaaatgcc ccgtcaataa ccatcggaac tacgcaggca ttggtgcgtc gcacgagaag
     2401 cgaaaagccc ggttttaccc tgtcaatttt cccgtcctct gttcgtgagc cttctggata
     2461 gagacagacg atattgccct gtcgaagaac atcaatgatg gccttcatag cagaaatatc
     2521 gggctgatcc tgcttgatgg ggatggtata aaacgaatcg atgagagctc cccagaatcc
     2581 tttaaacagc gtgtcgcgcg cgacaaaata gaatggccgc caaatccagt tctggcagag
     2641 gataggatct aaaaaacttt gatggctgct gagtaccaat acagggccgg cagaggggat
     2701 attttctctg ccccaaatcc gcatccggca gtacagttgg gttatgctga caatggcaag
     2761 ccgacagaat gtgtgccaga ctctccggac aagtccgctt ttatctttta tctttgccat
     2821 aggatttctt ttgtataaca gacagcattt tttcaacaac ggcttgggca tcaaggcagg
     2881 ttgtgtctat caccaccgcg tcggaagccg gtgcaagcgg gccgaccgag cgtttttcat
     2941 ccgaagcatc tcgaagcagg atttgctggg ccaaggcatc aaaatctacc ggcgtgccgg
     3001 cagtctgtaa ttctatgtag cggcgccgcg tgcgttcata aggatctgct gtaagaaaaa
     3061 atttatgctg cgcatcggga aataccactg ttccctgatc gcggccctcg gtaacgacgg
     3121 cttcaaagcg ggaagcgaaa tcctgctgaa gcttgaccag acgggcgcgc agctgaggct
     3181 gggaggcgat gtggtgcacc ttatcagtaa cttcgggcag gcggatgtcc tgcgtgcagt
     3241 cccggccgtt gatgaacacc ttcagcacat ccccctgatg gataaactcg aattgggttt
     3301 gttccatcac acctgcaacg gcctttgcat ctgtcaaatc ggctccggcc tgcatagccg
     3361 ccaaagtaac agcacggtag gtagcgccag tatccagaaa tacagcatta agccgtccgg
     3421 ccagcatccg tgcagctgtg cttttgccga ctccggccgg cccgtcaatt gtgattatgc
     3481 atctgttcat acctggctgc agctgcaagg ttggccgtgg gtgttgacac tgttgtcgcg
     3541 gcgaatagcc acaatgctcg tatcatcggt ctgattggcc agtcctacaa agcggcggcg
     3601 ataccccagc agactgccga taaactgctc ggctgtcgaa cgggggcatt gcgggagtgc
     3661 cgctaacaga cgctctttgc cccagttctg accgtcaaaa ttcatcgcat caatcagacc
     3721 gtcggtgtac atcaaaagca tatccccctg ttcaaacggg atggtgcgtg ttatgtaatg
     3781 tgcatcaggg agaataccca gtaccagtcc gccttcttcc agttcggata ttttatcgcc
     3841 gcgaatcagc agggccggct catggccgca attgcagtag gtcaatgtgt tgtcgtcgat
     3901 gtcaatacgg gctataaaca aggtgataaa ttctccgtca cggcattcat tgcaggcgac
     3961 ccgattgagt ttgtggatga tttcttccat actgtggcgt ccgtagcccc cgtcggcata
     4021 agcccggagc gtgccgcgaa acatgctcat cataatggcc gcagggacgc ctttgccgat
     4081 gacatcagag attccgacaa caagggattt ctgaccaacc tgcagaaaat catacaaatc
     4141 accaccgatt tggtagcagg gtttataaac agcggctata tccagaccgc acatgcaggg
     4201 ggctttttgg ggaatcattc gccgctgaat gactgctgcc agccgcatct gttcggccat
     4261 tttagcccct tcaattgctt gggcgtaaag tttggcattg gtgatagcaa tggcgcactg
     4321 ggctgctacc agccgtgcta cccgaatggc atcgcggtca aagcgggcag gttccgggct
     4381 atagagccgc aataccccaa tcggctggtc tttgaaaacc atcgccacgg tcagctgact
     4441 gatcaggcct tcggctaagg ttgcttccct atagataatc cggtcgtcaa tccgcatgtc
     4501 atcgatcact actgcttggc cttgaaaggc ctgcttgacc acaggatcgt ccttggtgac
     4561 cgggcctttg ttgcggtatt gctcactaag gccgtacgta ctgcgcatct tcagatcgcc
     4621 ttcttcttcg tccagcagcc gaatggagca ggcggttgta ttggtaattt taacggcggc
     4681 ttctgccagt cggtccaata ctgtctgaag ttcaaaatta ccggccacca gtgtagaaat
     4741 ccgataaatt tgttcttcta tcagatcttg ttttttctct aattcgttca ttttcttcaa
     4801 aaaaacacga ttttatcttt acaacggata cccattatac acaattctat tgaaaacaaa
     4861 agtaaaattg ctatctgcag attctatcaa aatatgctat aaatttgatt tgataatttg
     4921 tttgttcttt atttaagcat ttgaggatat attaaattgg aaatagatag aaaagtcggt
     4981 gagcaagttg tcctatttat cagcgttgtt aaatgggccg tattggggat gtttgtgggg
     5041 gggattgtcg gttgttcaac agctgttttt atcaaaacgc tcaattggtc tatttccttt
     5101 acatcccaat atccctatta ttttctgctg atgccggctg gttttctggc cagcagtctg
     5161 atagttaaat tattagcccc ccaagccgaa ggtcacggga cagaaaaaat aatcgaggcg
     5221 attcataagc agtcggggaa aatttcgccg gcagttgttc ctgtcaagct ggttgcgacc
     5281 attattacga ttgccttagg cggttcggct ggcaaagaag gcccggctgc gcaaataggg
     5341 gcggctttgg catcttgttt tgctgatctt ttgcggtttg atgataacga ccgcaaaaaa
     5401 ctggttatct gcggcatcag cgctggattt tcatctgtat tcggtacccc agtcggcggg
     5461 gctatctttg gtgtggaagt actgtttgcc ggttctatga tgtatgaagt acttttgcct
     5521 tcatttatcg ccggagttac agcctaccaa gtttcttcgg tcttgggtct gaactatttt
     5581 caacgtccca ttgaatttat accggtattc agcggctcat tttttattaa agtaatagcg
     5641 gcgggattct ttttcggact tgtttcactg ctttttattg aaataatgac ggtgacccat
     5701 aaagcggcca aaaagatacc gcttcagtta tggataaggc cgctgttagg tggtctgata
     5761 tgtgcaattc tggcattggt gttttcaagg gattatttag ggcttggtgt tgaaacgata
     5821 gaatcttgtc tgaaaggtat gccggtggtg tggtatgcgt ttcttatgaa agtcatattt
     5881 accagtgtta cgctggcttt cggcggcagt gggggaattg ttacgccgat tttctttatc
     5941 ggcgctgcgg cgggaaatac ttatggccac tttttcggtc tggatccggc agtattctcc
     6001 tcaatcgggt ttgtcagtct tctggccgga tgtgccaata cgccaatcag cgccagcatc
     6061 atctcgatag aactcttcgg ccctcagctt gctccctacg cagcggctgc ctgcatcgtc
     6121 agttttctga tgactggtca ccgcagtatc tattccagtc aggttctggc tatacgaaag
     6181 tctgcttcaa ttgatgtgca gattggccgg gaattaattg ctgtgacggc ttgtgcacat
     6241 ccccgagaaa aaagtctgtc ggcttttctt ctcagactga tcaaaaagct aaaaatagtg
     6301 ctcaagggtg atttccccca gaagcaggat tgaagaaaat ggaggcattg tatggaactg
     6361 tttgaagcta tcaagaaacg gcagagctac cgcggcggat ttacaaatca aactgtcccg
     6421 cgtgaagact tggtaaaaat tgtgcaggca ggactggacg ctccctcagg caaaaactgt
     6481 cagaccacta cttttgtgat agcggatgat ccggctgtgg tgaatcagat tgcacagatg
     6541 cataccacca acaaagccat gcagcaggca cgggctttta ttgcctgcat cattgataaa
     6601 aatcctgccc cgtcgtatga aggttatgcc tttcaggtag aggattgtgc ggcatcggtg
     6661 gaaaatatgc tgctggccat tacggctctg gggtatggaa gcgtctggat tgacggttgg
     6721 ctccgtcttg aaggccgcaa cgaggctatc ggccgactgc tgggagtgcc ggatgataag
     6781 atagttcggg tgcttttgcc ggtcggtatt ccttctgaag aatataagcg gcctgccaaa
     6841 aaggccttta atgagcgtgc ttggtttaac cgatatggct tatccggctg aacaatgtct
     6901 gcaatacctc aaaatctgcg atgtcctaaa tgtggttcac aagatatagc agaaattctt
     6961 tatggtccgg ttaagatgac tccagaatat atcaggatga ttcaggccaa gaaggtcctg
     7021 atttgcttca agccccaacc gaaaaactca ccccgatacc actgcaacta ctgcggatat
     7081 gagtggtagg ataaatcacg taaaactggg cttaatgttg actttgcgtc cgtaacggcg
     7141 tttgcgcgca ttgatttttc tgcctttccg agtggactga cgtactcgga aaccgctttt
     7201 acgagcccgt ttaactaagc ttttgcgatg gttttgcatt gtatcactag ttcctgaatt
     7261 cctgctgttc tcaactggtt cattcggcaa agcccgaaag acattagaaa atgaacgcaa
     7321 aattataacg gcgtgtttat cttttgcaag aggagtttga aaatcttaga aaaataaaaa
     7381 atcatacaaa tcaggtatgt tgtatcgcct tattgatggc atcagctaaa ctctgcggct
     7441 ggtctgttcc tatacggact tttttgccgt tcttaaatgt gatttctaca ccatccagac
     7501 cggaaatgtt gtataaccat cctctgatat accagcgtat tccccaaccc caccaccagc
     7561 agtttctgac aggacggcag tcggcaattt tatttacggg ataggttttc caaaatactc
     7621 cggggccgaa ttgaattcga atcctttcgt catttatggt tacagtaagt cctgaaagca
     7681 tcaagataat atatccccca agcagaggac agattgccag agcaatcttg tcatcgtgtt
     7741 gcaggcgact attaacagct tcgattatca agacggcata aagtccggta aaaacgataa
     7801 gaacaagata aaaggacaat ccgccgcgtt gggtatgctg atatttcagt gtcatttggg
     7861 gttttccttt ccaaattcga tgctctcctc tataataacg ggtcaaagcg ggaactgatg
     7921 cacaaaatcc gagcttaaaa gggacattta tgacgcattt gtttgtaaaa gatattcagc
     7981 cgggacgaca gattcaggat gtttacatgg taacccagcc ggtactgcga aacacaacaa
     8041 aaggggactt gtatattgca atgtatctgt ctgacaaaac aggcaaactg aatgcccgga
     8101 tgtggcaggc cacagagcag atatatcaat ccattcccag cgaaggattt attgccatac
     8161 gaggcaaaag cgaactgtat cagggaaatc tgcagattgt agtcaatgag gtgcaggtgg
     8221 tccagcccga tcaggttcgg ctcagcgact atatgccccg caccgagaaa aacattactg
     8281 aaatgtatga gcagcttaaa gggattctga aaacgatttg caacggcgat cttaagcgtc
     8341 tgaccgaagc atttctaaat gaccaagaat tgatgaagca gttttgcatt gctccggcgg
     8401 caatgcagat gcatcatagt tatctgggcg gcttgttgga acatacactc aatatgcttc
     8461 aagcggccca agcactgttt ggattgtatc cgaaaataca aaaggatttg gtactagttg
     8521 gcatttttct gcatgatatt gccaaaacgc aggagctgac gtataaaatg ggctttggct
     8581 acacagacag ggggcaattg ctggggcata ttattcaagg gactcaaatg attacccaga
     8641 aggccgatga tttgaaggcc gacggcacac ctgtggatcc gacagtcctc gacagcttgc
     8701 tgcatattat tatcagccat cacggccaat acgaattcgg ctcaccgaag ctgcctgcta
     8761 cgccggaggc ctttatggtc acttaccttg acaatcttga cgccaagatg aatcagactg
     8821 ctgcactgat tgaaactgat gccggcgacg gcggcaactg gaccagctac caaaaatcgc
     8881 tggagacaaa attataccgc gcccgggttt taaatagcga ttagcatgcc ggccggctaa
     8941 atgcttttgc ggcggatttt ggcgccgacg gcgtttagtt tttcttcaat tctgtggtag
     9001 ccgcggtcga tatgatagac acgctgaatc gttgtcattc cttcagcggc catccccgct
     9061 aaaaccaaag cagctgaggc acgcaagtcg gaagccatta ccggtgcggc gataagtttc
     9121 tttacgccgg ctacgatcac gctggggcct tccttgcgga ggttggccgc catgcggttc
     9181 aattctgcga catgcataaa tctgtctgga tatattttct cagtaatgac gctgttgccc
     9241 tcgccaaggg ccagcagggc cataaattga gcctgcaaat cagtgggaaa tcccgggtat
     9301 ggctgggttg tgatatcggc cgggtgaatg ggaccatcga agcttacttc gcagccgtca
     9361 tcgcaggatt ctacattgac gccaatatgc cgcaggcggt cggtaacagc catcatatgg
     9421 ttgagattgc agtgttggat tttcatctgg ccgcgtgtaa tagcggcggc aaccataaag
     9481 gtgccggctt cgatacggtc atggatgact gaccaggtta ccggtttgag cgatttgacg
     9541 ccttcaatga ccagccgggg cgaaccgatg ccgctgattt tagctcccat tgcattcaga
     9601 aaattggcta aatctgcaac ctcaggttcg caggccgccg actcgatgac ggttgttcct
     9661 ttggctgttg cagctgccat catcacattg tctgttccca atacagtcga gccgaaaggc
     9721 ccccctaaga aaatctgtgt gccgatcagc ccgccagcag gggcttcggc gacgatatag
     9781 ccgttttcca gatacacatt tgccccgagg gctttgatgc ctcgcaaatg aatatccaca
     9841 ggcctgtcgc cgattgcgca gccgccgggc atagagactt taaccctgcc gcagcgagcc
     9901 agcagaggcc cgagaataca aatactggcc cgcatttttc gcacgatatc atagtccccg
     9961 acaggattat cgataacgga tgcatctata tgcaatccgt ttccgtcccg ccagaccttt
    10021 gcacctaagc tctccagcag tgtttcaaga gagcgaatat cagccagatc gggagcgttt
    10081 gaaactgtgg aaatgcccgg tgccagcagt gccgcggcca gaataggcag agccgaattc
    10141 ttggagccgt ccacatttat cgagccagcc aaacggaccg gcccgtcaat ttcaaaaatg
    10201 tccataagcg attccttgca aaatagattg gaatgtacta taacacccaa agtgctgcaa
    10261 agcaagatga gattgacgat aaaacgcagc aaaggaattc agtcgctcag tcatgcggga
    10321 tttctggact attctggcag gtatcgcaca agaaaggcag gctgtattat ccgaagaaac
    10381 cgagccgttc tgggatgtct tttcgataag tatgaccgta cttggtttgt tggtatcagc
    10441 tgtctggatg tgtcaattcg gcggcttcag tgcactgggc aaggccccca tccgacggca
    10501 tcgtatgccg gcagggctgc cgatggtgct gctgggaact tggattctca tgctggcggc
    10561 attgtcggga ctgctccgca gcccatccga caatttggct cgaagccgtt tcctttattt
    10621 ggggatggct gtgctggaaa tcattctgat tgctgtgatg ctgacattgg cgtatcggtt
    10681 gtttgcgcgg cgtcttaaag gctttggttt tgactttcga acacttctca aagatgccgg
    10741 ttgggcgggt cttaatctga taggaattta tccgtttatt ctggccggat tatggataat
    10801 actgcgaatc ggccggctgg tgaatccaga tttttcactg gaggttcatg aaagtcttca
    10861 gatgctgacc gatggatctc tcaccttaaa acttcttgta gccggctttg ctgtgggagt
    10921 tgtaccgata tttgaggaaa tgctgtttcg gggatttctg caaacgcaga ttcgtacttt
    10981 aaccaaccgg ccatggctgg ctgtcgtgat aacttcggtg atatttgcaa ttctccatcc
    11041 gacaacccat atcgccgcct tgtttatgtt atcctgcgga ctgggttatg cttatgagcg
    11101 cagcggctca ctattccggc ccattttgat gcactttttg tttaatggaa ttagtgttgc
    11161 aatgaccctg ttaatgccgc ttgcttgagc attgacacaa cttagtccga taaatcggtt
    11221 aatgctggta aatccctgta aatccttcat cgcataaggt ctaatcttta attttaaggt
    11281 tatgtttgac aatataccga ccgatgtttt ggatgaaaat gtcttgaatg cgggagatat
    11341 gcatatgtcg gtacgacggc agggattaac gctgatggaa ctaatggttg tcattttaat
    11401 tgcggccatc ctgtccgcgg cggcagttcc tttgcttcga ggcaaaatcg aacggagcaa
    11461 atgggcggaa gcgaatgcgg ccgctggtgt catacgcaat gctgtgaagg tttattttct
    11521 tgaaacagga gatactttga ccggcaatat ggctgatgcg gcggttctta atgctctcca
    11581 tctggaagca gctgatatca caggcaccta ttttacggcc tctgattatg tcatcgataa
    11641 cgtcgatacg gatggcacgg ctgtgataac ggttacagcg agcaagacca acgccccttc
    11701 gggcagtaaa aaacttacag cttccggctc atttgaataa tccggatatg gggtggcacg
    11761 gagtattttg gaatacctct tctatgctgg ttccgggttg atattccagc aaacatttca
    11821 actgcaattc tattttttat cccatttctc catttaagaa ggttgacaga aggggatttt
    11881 ttggatagta tccgccggta aattgagcag gagaataaaa tggacaaggc aaatcctaat
    11941 ttaagggtag aatttcacgg tttaatagta gtggctaccc tgacagatga aaaaattctc
    12001 gaggagtcgc agctgaaggg gctggaaggg tcatttatgc cgctgatagc tgaaactccg
    12061 gctatccagt taattgtgga tttttcaaat gtacaatttt taaccagttc tgtcctcggc
    12121 ttattaattc gcatcagcaa aaaaatctat gagaaacagg gcaaacttcg gctctgttca
    12181 attaatccca aaattcttga aattttccgc attactcgtc tggataagat ttttgagata
    12241 tttcccaatg tggatgaagc gatgataggg ctgaaacgct cctaaagcgg ggtagtcgga
    12301 aatacgataa tatgtaagct taggttttgt atctatggag cgattaaaag acaaaaaagg
    12361 tatagttttt tgaaagagat tattccttgg ctgaagtctc aaaaattctg catcaatcgt
    12421 gggagattca cagcacggaa tccgaagcaa aacggattat tcagacactt ctttccacct
    12481 tgcaggaaca tgcgttcagc gaggatatga tatttgccat ccatctttcg ctggaagagg
    12541 cgtttgtcaa tgccatcaag catggcaatc attccgaccc ggaaaaaatg attaccgttc
    12601 attgtttgat taccccggaa aaattcgaca tttcaattgc cgatgaagga tttggttttg
    12661 atccgcaggg aatacccgat ccccgcagca acggcaacct ttataaatgt accggccgag
    12721 ggatcctgct gattcaatcc tatatggatg tagttgagta caatgaccgc ggcaactgca
    12781 ttcacatggt aaagtatcga aactctcaaa agcccgtcac tccgaaaacg acccagtcat
    12841 agcgtttacc gcttcttata aatcagccaa aaaagagtac ccaattgctt ctggatgatg
    12901 tattctataa atgcattttc ctgtaaaata gtttagtaat gtcttgatac acaaagatat
    12961 ttataggcac aagcagggaa aggattgggt atgggaaaaa tagaaaagtt tttcatggct
    13021 gctgttattt gcattgcggc ggcggaatgc ctgccggcag tccagacaga gccgaatagt
    13081 ccggccgaac tgaatatcgg tttcggcatt gccgcctata acagcgtata tgaaggcgac
    13141 gatgacaaag tgcttgcggt gccgatgctg ttttacgaat ccgaaaaaat ctttttccga
    13201 ggacggatgg gagggtatcg cttctttggg gatcagacct tttctgttgc cttgataggg
    13261 cagtggcgag tggacggcta tgatgacaat gacagccgtc atttgcgggg aatgaaggac
    13321 cgcaagatga cattggacgg cggactttcg gcaacctatg ttgacggctg gggtgcgaca
    13381 acggtatcgc tggtaacgga tttgctgggt cgccacaatg gtcaggaatt ctcaatcagc
    13441 tacggtaaac ggttttcatc cggccaatgg tctttcattc cgtcggcagg tgtgctctat
    13501 aagagcgctg atttaacgga ttactattat ggagttaaag ccggcgaagc tgctgcacag
    13561 cggcctgcct attcggccga tgatgtatgg gatccttttg tggctgttca gacgagtgtc
    13621 aagataagcc ggcgttggtc tttcttggca gcgctgcgtt atgaatggct gggcagcgag
    13681 attaccgaca gcccgattgt ccagagtcat taccagacac ttctgatggc cggaatgttg
    13741 tgccgatttt agacagactg acataaaaat tgatttgaag gcgttttact tatgtattaa
    13801 agtagagcgg caatcaatga tataaatgag tgaatggcag ggcggcaaat aaaacgattt
    13861 tagtcggaag aagcgtaaag ccgaattatc ttgttttatc ccgattcttg caagtttgga
    13921 gaatcgaaga ttttccaaac ttcgtatccg caaagagttg tgcgagaaaa gaataaaaat
    13981 tgcagtaggc ttttaggagt ttgtctatga aaaaatggct ttttgggggc atgatagtgt
    14041 ttgtcatcgt gtggaacgga tgtgcagcgt ccccgcaggc agttcagcag catgaagcat
    14101 tgcagccgca gcagattcgg gcggttatgg cttctgttgc tgattggcag ctggctcatc
    14161 ccagtgagcg tccgacagca gactggacac atggggcgct ctttgcagga ttgactgctt
    14221 gggctaatat ggccggcagc gacgcctatt tcaaatcctt gcttgctttt ggcgaaaaaa
    14281 acaattggca gccgtatcgg cgcccgtatc atgccgacga ccattgtgtc ggccagatgt
    14341 atattgattt atacaagctg tataaagatc ccaagatgat agcgccgatt cagcagcggt
    14401 ttgatttcat tttggctaat cccccggcca gcgagttgaa aatggtccgg gaaggcaatc
    14461 aggaccgctg gaactggtgc gatgctctct ttatggcacc gcctgtatgg gtcaagctgg
    14521 ccggcgtcac cggacagcag aaatatctgg attatatgaa taaagaatgg tgggccacaa
    14581 ccgattatct gtacgataaa aaagagcatc tgtattaccg agacagccgc tattttgacc
    14641 agcgggaagc caatggccag aagatcttct ggtcgcgcgg aaacgggtgg gtatttgcag
    14701 gattggcccg ggtgctggaa gaaatgcccg aagactactc cgaccgagag cgctatgtgg
    14761 aactatacca gcaaatggct gcaaagctga tttctataca gccccgcgac ggtctgtggc
    14821 gggcaagtct tctcgatccg caaagctatc cgacaccgga aagcagcggc agcggctttt
    14881 tctgttatgg actggcatgg ggcatcaatc atggatactt agatgaaaaa acatatctgc
    14941 cggctgtggt gaaggcctgg caagctctgg ttggctgtgt tcatccggat ggcaaactgg
    15001 gatatgtcca gcctatcggt aaagatccta aacaggtcag cgcagatctg acggatattt
    15061 acggcgtagg tgcgtttctg ctggccggaa gcgaagttta taagatatcc gttcgaaacg
    15121 gtgcggccgc tgtaaaactt aacatcgcca atcctgctgt gattttccgg gataatgaaa
    15181 cagtcgaatt ggattggagc cgtctgcaaa aggcggtgcg gggattgtcg aaggaaaacg
    15241 ccgctgtctt tgattttaaa accaatcggc ttctggttac acagtttatt gacgagggca
    15301 aaagcacaaa actgctgttt caaagttcat ttgctccggg cgaaaaaaaa tatttctggg
    15361 taatgcccca gccgcccgct ctggaaaagg ctgtttctga atatacgact tactgccgct
    15421 ttattcctga gcgaaaggac gattttggat gggaaaatga taaagttgca tttcgtatgt
    15481 acggtccggc gctggaatac gaaaccatta ccagcggcat tgatgcatgg ggcaaatgcg
    15541 tgccaacact ggtaattgat aaatttatta aaaattatgt cgaaaagcag attccttatc
    15601 accaagacca cggcgaaggc ggggactttt acaaggttgg accgaccctc ggctgcggcg
    15661 gacttgcacc gtttgttgac ggcaaggtta ttctgccccg caattttgtg caatggaagg
    15721 tgctggcaaa cggcccgatt cgctcgctgt ttgaactgag ttataatcct tggcaggccg
    15781 gctcggcttc cgtcagtgaa gtcaagcgta tttccatcga tttgggcagc aatatgaacc
    15841 gcattgaatg tacctacacc agcccgaatg cccaaaccct gccgctggcg gccggcatta
    15901 ttctgcgtga gaccagcgac cgcaaatgga cgtcggacca aaccattgcc tactggctgc
    15961 cgacggactt cattacaggc aatatgggct gcggtgttgt ctttggagca gactataaaa
    16021 caaataccat cgaggcggac agccatctgc tgttgactct gtcccagaaa gtcggaagcc
    16081 ctgtggtcta ttatgccggc tcctgctggg ataaaaatga ggaattcagc gattttgaaa
    16141 aatggcagca gtatctggtg aattttaaga agcggataga caatccgctg gttatcaaga
    16201 tgaataagta aagcaaatcc aaaaaaggga aatactgtta tggaagtccg atatgcacct
    16261 gatcctgtgc gttttagcag gatgacaaca caggaaatac gagacaattt tctgatagag
    16321 aatctgttta ctccgaatcg gatcgatctg gtttattcag atgtggatcg ggcgatcgtg
    16381 ggttcggcag tgccgaaaga aaagacgctt gaattatcat ctgccgagga attaaaggcc
    16441 gagtttttct gtcagcggcg tgaattgggc gtcttgaata tcggccaaag cgggaccgtt
    16501 gccgtcgacg gccgaacata tgcactgggc agcaaggact gcctttatat cggacggggc
    16561 tccaagacaa tctcgtttgc cagcagcgat gccggccgtc cggccaaatt ttacctgctc
    16621 agctatccgg ctcataaaga atatcccgcc gctcatatca aaatagagcg ggccaatccg
    16681 attcggcttg gctcgatcga aacagccaac aagcggacta tttatcagtt tattcatcct
    16741 aaaggtgttc aaagctgtca gttggtcatg ggatttacgg tattggaacc gggatgtgtg
    16801 tggaatacaa tgcctgccca tacgcacgag cggcgcatgg aagtttatat gtattttgat
    16861 atgccggaca atgtaagggt ttttcacctt atgggccgtc cgaatgaaac ccggcacatt
    16921 gtagtcaaat cggagcaggc agttattgcc cccagctggt ctattcattc cggtgcagga
    16981 acaggcgctt acagtttctg ctgggggatg ggcggggaaa atcaggcctt tgatgatatg
    17041 gaccatttga caatggatga tataaaataa gatgcaggga gtatgcaatg atattggatc
    17101 aatttaagct ggatgggaaa aaagcaatcg taaccggcgg tgcacgggga ctgggtcagg
    17161 ctatagccgt tggacttgct gaggcagggg cagatatagc gctggcggat gttctggata
    17221 tgtccgaaac aaaggggcag attgaaaaac tgggacgccg gtgtattgcg attacggctg
    17281 acctgagcag aaaagaatct gccgacatta ttgtcagtca aaccgtcaag cagcttggag
    17341 gaattgatat tttgttcaac aatgccggca tcatacgccg ggccccattt acggaattca
    17401 gcgagaaaga ctgggacgat gtgatgaata tcaatattcg ggggctgttt tttctgagtc
    17461 aagcggccgt caaggtgatg attcagcagg ggcggggcgg taaaattatc aataccgcct
    17521 cgatgctcag ctttcaaggc ggcattttgg tgccttccta tactgcttct aaaagtgctg
    17581 tgatgggttt gactcgtctt atggcctgtg aattggctaa atacaacatc cagacgaatg
    17641 ctatcgcccc cggctatatg gcaaccgaca atacaaaacc gctgcgggat gatccggttc
    17701 gcaataaagc cattcttgac cgaattccgg ccggacgctg gggaacgccg gaagatttga
    17761 aaggagtggc ggtttttctg gcgtctgcgg cgtcggatta tatgaatggc tataccgtag
    17821 ctattgacgg cggctggctg gcaagataat aaatattcgc cattttttga ccggctgaat
    17881 cctaaattgg tatctcgttg ggacagttat tggattcggc cggtcttttt tgctgaggca
    17941 atgaaaaggg atttaaaatc tgtgttttga tgaaacaaat taccatttct attcgtcata
    18001 ataattgttt attgcaggtt ttcacacaaa gacagtctat ccaaataaag ggaatgcgta
    18061 tcttttttgt ttgttaagcg aatcggataa gacagctggt agtaaagctt cgaaagggta
    18121 caggtgagaa aaaaactcgg acaactgctt gctgaaaaaa aatatatcag ccaagcacag
    18181 cttgcatcgg cactgcagcg gcagcagaaa gaccgccata agctggggca gattctgcag
    18241 gaagaaggcc tgattaacac ccgtcagctg acggaagccc tttcggaaca ggcagggata
    18301 gaaaagctgg atgatgttga aaaaattccg attgacaaag atattatatc actggttccg
    18361 gcagaagtag tcagccaata tcgcctgctg cctatcagcc gccaaaacgg ccggctgacg
    18421 gtcgcgatgg tggatccgtt cgatgctgag gcgatagcta atctgcgttt attaacagga
    18481 ctttctattc gccggtgcta ctgctcgccg gcggaccttg aaaaagcaac ccggcgtcat
    18541 tacggcagca atgtagcccg catggttaat gatttggctc cggctgaaaa aaaggccgcc
    18601 cagacggttg aaacaatcga tactgcgcag atgactaccg cccggctcca cgagctggca
    18661 cgagaaccct ctcttgtaaa tctggtcaat ttgattctgc tggaagccat cgaagcgcgt
    18721 gccagcgata ttcatataga accgtttgaa accgaaatta aaattaagta ccgcattgac
    18781 ggaatgctgg ttgagaaatc ctcttctccc aaacggctgc atccggcgat tgtatcccgc
    18841 atcaagatta tggctgcgat gaatatcgcc gagcggtttg tcccgcagga cggccatata
    18901 gagttttcag gacccaaggg caagattgac atccgggttt ctactgtgcc aactattttc
    18961 ggggaagccg taacaatgcg tctgctggat cgcagtgcag cattgattca attgagcgat
    19021 cttggaatga gcccccatac gctggccggt ttctcgcaat gtttaactaa gccgcacggc
    19081 attgttttgg ttaccggacc gaccggcagc ggaaaaacca caacgcttta cgcggcgctg
    19141 aataagattt atgaccccag cttaaaaatt attaccattg aagatccggt agagtatcag
    19201 cttgatggcg ttgttcaaat gccggtcaat ccgcgtcgcg ggctgacatt cagccgcggt
    19261 ttgcggcata ttctgcggca ggatccgaat attattatgg ttggcgagat acgcgataaa
    19321 gaaacagcag atatcgccat tcgggcagca ttgaccggac atctggtttt ttccacactg
    19381 cacaccaatg atgctgcggg agccgtaaca cggctgattg atatgggagt cgagccgttt
    19441 ttgctggcca gttcgctgga aggtgttttg gcccagcggc ttgttcgcaa aatctgtccc
    19501 ttttgccgtg tgccgtatca tccggcggaa agtctgctga acagcttaaa caattccgtt
    19561 cgcatcccgc tggatgcggc gctttatcac ggaaccggct gcgatgaatg caatcatacg
    19621 ggcttaagcg gccgcatcgg gatttttgaa ctgctgcgaa ttactgatac gctgcggaag
    19681 ttgattgcgg aacggccgac gacagagcag attcttgcag cggctccgtc tgatcatgtc
    19741 agtatgcggc atgatggaat tgagaagatt ctgtcgggat taacaacacc cgaagaagta
    19801 ctgcgggtta cccataccgt cgaagaagaa taaccggcgg cagagcaaac gtcctctgca
    19861 aaggatggac aggcaaaagg aaccgagtgg caatgtttca atacaaaggg attgatgcag
    19921 cgggacgacc cattgcagga agcattgagg ctgtggatcg gaaggcagcc atcgctgagc
    19981 ttgcggggca gggatgcttt gtccgccaga ttcaggaatc ggctctcgat gcggccggca
    20041 cgataggaac aatacagagc gggatttcaa ggtccgggat taaggccaaa gatattttgg
    20101 caatgacggc tcagctggcc accgcactgg aagccggctt gccgatgttt agcgctctgg
    20161 aaatcctcaa atcgcagcag aaaaaagcag cgatgcttaa tctgctgaat gaattgacag
    20221 gagccgtcag ctccggtcaa tcgctcagcg aggcattggg acggcatccg aatttatttt
    20281 caagtcttta tctgtcgatg gtgcgagtcg gggagacagg cggcattctc gaaaaaacaa
    20341 tgcagcagct ggtaggtttg atgagtcggg aggagaaaat caggtccaat ctaattagtg
    20401 cttccgctta cccgctgttt gttttgtcgg ccggcttgat ttctatggtg attattctgg
    20461 tctgggtgct cccccggatt atccgaacga taggcattga tccctcgatg ctgccgctgc
    20521 cgacgcggat gctgatgggg tttagtcatt ttctgctggc ctttggatgg ctggcagcgc
    20581 tgggtatagg atttgccgtt tattgtttta tacggtggaa gcgcaccccc ggcgggcgaa
    20641 tgacgtggga cagcttcaag ctgaagcttc ctctgttcgg caatgtagtg cggtctctgg
    20701 ccgtcagcga accaacgcta cgggcaaaac gccctaccgc cagcgaccga actacattgc
    20761 cg
//
LOCUS       DAOTBO010000043        20155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2194_length_20155_cov_5.490000, whole genome shotgun sequence.
ACCESSION   DAOTBO010000043 DAOTBO010000000
VERSION     DAOTBO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_20155_cov_5.490000"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /gene="rplO"
                     /locus_tag="PLE88_06620"
     CDS             complement(<1..293)
                     /gene="rplO"
                     /locus_tag="PLE88_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOM76172.1"
                     /translation="MLSNEITQNVGAHPKRKRVGRGRGSGHGKTCGRGHKGNASRSGS
                     SMPLTFEGGQMQLFRRLPKRGFNNAQFSVRYEVVNLSQLERVFSDGTDVGLNEL"
     gene            complement(301..792)
                     /gene="rpsE"
                     /locus_tag="PLE88_06625"
     CDS             complement(301..792)
                     /gene="rpsE"
                     /locus_tag="PLE88_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOM76173.1"
                     /translation="MQTEQPLEDTVVKVFRNAKVVKGGRRFSFSALVVVGDRNGTVGY
                     GYAKANEVPPAVEKAIKDAKKNLHQVPIVEGTIPHPVIGKCRATQVTLLPASPGTGVI
                     AGSAVRAVLEYAGVRNVLTKVYGSRSAKNVVKAVMNGLMKLRDKETVEALRGVGLEVE
                     KRW"
     gene            complement(852..1202)
                     /gene="rplR"
                     /locus_tag="PLE88_06630"
     CDS             complement(852..1202)
                     /gene="rplR"
                     /locus_tag="PLE88_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOM76174.1"
                     /translation="MKQTRIRRNWRSRRKIFGTPERPRLVVFRSSRHIYAQIIDDVAG
                     ITLVSASTRAKALRDSIPYGGNQNAAKGVGTAIAKQALDVGIKAVCFDRNGYKYHGRV
                     KALAEAARQAGLAL"
     gene            complement(1284..1850)
                     /gene="rplF"
                     /locus_tag="PLE88_06635"
     CDS             complement(1284..1850)
                     /gene="rplF"
                     /locus_tag="PLE88_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOM76175.1"
                     /translation="MSRIGKKPIAIPAGVSVEQKGCTIKISGPRGTLERQIQPDIQVT
                     VDGKQIVVSNPDSASRFKKALHGTVRAHLNNMVIGVSKGFEKAMQIYGTGYNVKEQGG
                     KLVLSVGYCHPVELPIPKGVKVDIKTPATRGNDVPALFTLTSADKQLLGQFAAEIRKV
                     RPPEPYQGKGIRYADEHVIRKEGKALGT"
     gene            complement(1851..2249)
                     /gene="rpsH"
                     /locus_tag="PLE88_06640"
     CDS             complement(1851..2249)
                     /gene="rpsH"
                     /locus_tag="PLE88_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOM76176.1"
                     /translation="MSLSDPIADMLTRIRNAARVRRQQVYVKANKVCQGVAQVLKDEG
                     YITDFDRIEDAHQGLLRIQLKYTPEGDPVITSITRVSKPGCRVYRGKDELPRVMGGLG
                     IAIVSTNLGLMSDRKCRQHNVGGELICTVS"
     gene            complement(2292..2477)
                     /locus_tag="PLE88_06645"
     CDS             complement(2292..2477)
                     /locus_tag="PLE88_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOM76177.1"
                     /translation="MSTKALENKARKKPKYRSRAYTRCQICGRARAVYRKFKICRICF
                     RKMANEGKIPGVKKASW"
     gene            complement(2507..3049)
                     /gene="rplE"
                     /locus_tag="PLE88_06650"
     CDS             complement(2507..3049)
                     /gene="rplE"
                     /locus_tag="PLE88_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOM76178.1"
                     /translation="MARLKDLYKSKIVPALREKYAYSSPMAVPRLQKIVVSMGVGKAV
                     QDKKYLENAIKDLTVITGQKPLICAAKKSVSNFKVRQGDKTGLKVTLRRDRMYEFMDR
                     LINLAIPRVKDFRGLNPNGFDGHGNYSMGLDEQSVFPEIDPARIETQQGMNITFVTTA
                     QTDDEARDMLKLFGMPFKAN"
     gene            complement(3079..3417)
                     /gene="rplX"
                     /locus_tag="PLE88_06655"
     CDS             complement(3079..3417)
                     /gene="rplX"
                     /locus_tag="PLE88_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOM76179.1"
                     /translation="MARHVKKGDTVVVIAGDSKGATGKVMRVIPDKQRVLVEGVNLAY
                     KHVRPSQKNPQGGRIRVERPIHISNVLPVNPKGTKGTRVRFTVDEKGNKQRVAADGTE
                     IGIVTRAAKK"
     gene            complement(3449..3838)
                     /gene="rplN"
                     /locus_tag="PLE88_06660"
     CDS             complement(3449..3838)
                     /gene="rplN"
                     /locus_tag="PLE88_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOM76180.1"
                     /translation="MIQQETMLEIADNSGVKTAQCIKVLGKCSSHKGKYTRPTATVGD
                     IICVAIKKHLPNCQLDRKKVYRCVIIRTKYPVRRPDGSYVRFDSNAAVIIDADKNPVG
                     TRIFGAVARELREKNFMKIVSLASEVV"
     gene            complement(3851..4117)
                     /gene="rpsQ"
                     /locus_tag="PLE88_06665"
     CDS             complement(3851..4117)
                     /gene="rpsQ"
                     /locus_tag="PLE88_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOM76181.1"
                     /translation="METKKDELRTRQAVVVSKSGNKSIVCQIDYKVRHPHYGKYIRRR
                     TKLGVHDPGNLAGVGDVVEITECRPISKTKSWRLVKVLKKAVIE"
     gene            complement(4122..4319)
                     /gene="rpmC"
                     /locus_tag="PLE88_06670"
     CDS             complement(4122..4319)
                     /gene="rpmC"
                     /locus_tag="PLE88_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOM76182.1"
                     /translation="MKPSEIRDMRPDERQEKLSELARQLFTLRSQAVTENLENRHAIR
                     NVRRDIARIKTIIRQDQIKGR"
     gene            complement(4337..4759)
                     /gene="rplP"
                     /locus_tag="PLE88_06675"
     CDS             complement(4337..4759)
                     /gene="rplP"
                     /locus_tag="PLE88_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOM76183.1"
                     /translation="MALMPKRVKYRKHMRGKNKGKASRKNFVAFGEYGLQSLEASWIT
                     GRQIEAGRIAATHFLKREGKVYIRIFPDHSYTAKPLEVRMGKGKAEVEGWVAVVRPGT
                     VLYEIGGVDEDVAKRALLRVSHKMPVKCRFITRRHSLG"
     gene            complement(4784..5569)
                     /gene="rpsC"
                     /locus_tag="PLE88_06680"
     CDS             complement(4784..5569)
                     /gene="rpsC"
                     /locus_tag="PLE88_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOM76184.1"
                     /translation="MGQKTSPIGFRTGITLPWQSNWFAPKANYGEFLVEDYKIREFVD
                     KKFNRQPPYAAVSKVEIARTRNEVKVTLHTARPGMVIGPRGAEVDKLREELEALIDRK
                     ISVNVIEIKEPALDAKLVGESIAEQLKKRAAHRRVMKMTCENAMNAGALGVKIICSGR
                     LGGAEIARSEVQKMGSIPLQTLDANVDYAVSTAFTTYGAIGIKVWIYKGKFGQEITPV
                     VQSKPRRTGKGGAEKRSGRSSSRQGEADWGADGAADSNVEVNE"
     gene            complement(5591..6139)
                     /gene="rplV"
                     /locus_tag="PLE88_06685"
     CDS             complement(5591..6139)
                     /gene="rplV"
                     /locus_tag="PLE88_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOM76185.1"
                     /translation="MLSGKKLTQVCRERAIQDSQLADHLARGGMSRDEALSAVRKWKK
                     GLYTPKPGKEDVERLAAALGVEVADISEWRASYRYAPMSARKVRLVTQLIAGRPVQDA
                     LDLLTFTNKRAAPTVKKVLQNAIANADEQEADVDALVVSEARADQAGRRLGTKQWFPK
                     DRGRAHPIRKEASHIHITVSEG"
     gene            complement(6182..6454)
                     /gene="rpsS"
                     /locus_tag="PLE88_06690"
     CDS             complement(6182..6454)
                     /gene="rpsS"
                     /locus_tag="PLE88_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOM76186.1"
                     /translation="MGRSTKKGPYVDEKLYLKVRKQADTGSKEPIKTWARACTIIPEF
                     VGYTFMVHNGKIFHKVFVTEDMVGHKLGEFSMTRTFKGHSGGGAAK"
     gene            complement(6506..7360)
                     /gene="rplB"
                     /locus_tag="PLE88_06695"
     CDS             complement(6506..7360)
                     /gene="rplB"
                     /locus_tag="PLE88_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOM76187.1"
                     /translation="MAIRIYKPTSNGRRNASVIDYKAVLTTDKPEKSLCRRIRKTGGR
                     NHTGEITVRFRGGGARRIYRIIDFKRNKDNIPATVQSIEYDPNRNCFISLVQYADGEK
                     RYILAPEGIRVGMTVESGPAVEPRIGNAMPLAAVPVGVEIHNIEMNAGQGGKLVRTAG
                     GVARLMAKEGDWATVVLPSGEMRMVRKECRATIGQLSNGDYQNVKIGKAGRKRYMGRR
                     PHVRGKAMNPVAHPLGGGEGRANGGRHPCSPTGVPAKGGKTRNPRKVSSKRIIRRCKS
                     NRGVQLVL"
     gene            complement(7397..7675)
                     /gene="rplW"
                     /locus_tag="PLE88_06700"
     CDS             complement(7397..7675)
                     /gene="rplW"
                     /locus_tag="PLE88_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOM76188.1"
                     /translation="MDDYSIIIRPLVTEQGMHFANAKNAYSFEVNKKANKIQIRNAVE
                     RLYGVKVTDVRTANVKGKPRRRGRYIGTTKSWKKAVVVLHQDYHIDLF"
     gene            complement(7694..8323)
                     /gene="rplD"
                     /locus_tag="PLE88_06705"
     CDS             complement(7694..8323)
                     /gene="rplD"
                     /locus_tag="PLE88_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOM76189.1"
                     /translation="MIDVKVYNREGKELESVQVDEALFGGTVRYPLLKQAIVMYHANK
                     RVGTAASKNRGLVEGSTRKLYKQKGTGHARAGTVRTPVRVGGGVAFAKAARDFSHDMP
                     KKQRRLACDSALLAKMLAGRLVVVDELTFDAPKTREFARILGNLQIDRSCLVTISRQD
                     VNLYKSARNVPKVDVMTVSDLNAGDVCRKQKVLFTKEAFLSLLDKKKSN"
     gene            complement(8357..9013)
                     /gene="rplC"
                     /locus_tag="PLE88_06710"
     CDS             complement(8357..9013)
                     /gene="rplC"
                     /locus_tag="PLE88_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOM76190.1"
                     /translation="MVMLLGKKVGMTQIYDDAGKMVSVTVIQAGPCKVMQVKTPAADG
                     YAALQLGYDDVKKNRILKPAQGHAAKAQTAPKRFVREWRLNDADTPPYGLGDEVRVSA
                     FAGVKYVDVSGISKGKGYAGVMKRHHFGGFPASHGTERKHRAGGSIASHASDRGHGGN
                     LKKGKRMGGRMGGKRVTSKHNIVVAIDEENNLLVVKGAVPGPSGSYVEVRSSHTRPQK
                     "
     gene            complement(9105..9416)
                     /gene="rpsJ"
                     /locus_tag="PLE88_06715"
     CDS             complement(9105..9416)
                     /gene="rpsJ"
                     /locus_tag="PLE88_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOM76191.1"
                     /translation="MTDTERIRIRMEAYDHRALDSSAREIVEQARRTNARVSGPVPLP
                     TRIERYTVLRSPHVDKKSREQFEMRTHKRLIDIYDANSRTVEALNRLVVPTGVFVRIK
                     A"
     gene            complement(9795..11876)
                     /gene="fusA"
                     /locus_tag="PLE88_06720"
     CDS             complement(9795..11876)
                     /gene="fusA"
                     /locus_tag="PLE88_06720"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOM76192.1"
                     /translation="MAADLSKVRNIGIMAHIDAGKTTVTERILFFTGKTYKIGEVHEG
                     TAVMDYMQEEQERGITITSAATKCPWKNHIINLIDTPGHVDFTAEVERSLRVLDGAVA
                     VFDASEGVQAQSETVWRQGQKYHLPCICFINKMDKIGADFEMCIESIRNRLLAYPVAV
                     QIPIGAESDFAGFVDLLTMKAVYFKTEETGASIYEKAIPPSLVEKAEQMRHRMIEAAA
                     EFDEALMHAYIHDEPLTEERITAGLRKGTIAGKLHPVLVGAALKNMGVRRLLDAVAMF
                     FPSPLERPPAVGYKKGDQKQPVQVLCDPHKPFVGLSFKIMTDKHGDLYFIRVYQGTLK
                     TGTRVLNINRDRKENITRIFEMHANSRQILESAQAGDIVAVVGLKETLTGDTLCDTHF
                     PVMLESITFPETVISMSIEPRSASDRSKLSEALVSLKREDPTFDFKFDQETGQTIISG
                     MGELHLEVLEHKLVRDMGVNVRVGKPRVAYREAISQTAQAEGKFIRQTGGRGQYGHVV
                     LTVEPFFEENGRYSRKNEFINAIIGGAIPKDYIPAVERGAMEGLASGALSGYPIVGAR
                     VKLIDGSFHPVDSSEIAFEQAALIAVREAVQKAGPILLEPIMRVQVIVPEANYGAVQS
                     GLVAKRAEIVDSRLHGNMRVIDAKVPLAEMFGYSGQIRSATAGRGSFTMEPLDYEKVP
                     EQISEKILLGY"
     gene            complement(12039..12512)
                     /gene="rpsG"
                     /locus_tag="PLE88_06725"
     CDS             complement(12039..12512)
                     /gene="rpsG"
                     /locus_tag="PLE88_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOM76193.1"
                     /translation="MAKKFTASYTQLKPDPKFNSKLVAKFVNCLMWDGKKSAAYKVFY
                     DAMDQVAKKIKDVPPLEVFETAVNNVKPLLEVRSKRVGGASYQVPMQVGPKRQQSLAF
                     RWILAAARGKKGKPMSERLASELVDAYNKEGAAMTTRENVHRMAEANKAFAHFAW"
     gene            complement(12543..12908)
                     /gene="rpsL"
                     /locus_tag="PLE88_06730"
     CDS             complement(12543..12908)
                     /gene="rpsL"
                     /locus_tag="PLE88_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOM76194.1"
                     /translation="MPTINQLVRNGRSRKGKKRISDISGSPQKRGVCLIVRTQTPKKP
                     NSALRKIARVRLTNGKEVTAYIPGIDHNLQEHSTVLIRGGRVRDLPGVRYHIIRGVLD
                     TAGTANRKQGRSKYGTKKS"
     gene            complement(12938..17206)
                     /gene="rpoC"
                     /locus_tag="PLE88_06735"
     CDS             complement(12938..17206)
                     /gene="rpoC"
                     /locus_tag="PLE88_06735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOM76195.1"
                     /translation="MVESIYDRINDYGSVKIALASPNDIRSWSFGEVRKPETINYRTY
                     RPEKDGLFCERIFGPERDWECACGKYKGTKFKGIICDRCGVKVTHSRVRRKRMGHINL
                     AAPVAHIWFFKALPSRLSTLLGMKTQDIEKIVYFQDYVVTDAGQSPLKVKQLLTEDEY
                     RESLSKYGNSFRASMGAEAIKELLQKLDLEALSAELRQEISETNSKQKIKDLTKRLKL
                     VNAIKSSENKPEWMILDVCPVIPPDLRPLVMLESGNFATSDLNDLYRRIINRNNRLKK
                     LIDLNAPEVIIRNEKRMLQQAVDSLLDNSRCRRPVLGSNNRPLKSLTDMIKGKQGRFR
                     ENLLGKRVDYSARSVIVVGPNLKLYQCGLPKKIALELFQPFIIRKLKDRGLADTIKSA
                     KRMLERREEHVWDILEEVIHQHPVLLNRAPTLHRMGIQAFEPVLVEGNAIMLHPLVCG
                     GFNADFDGDQMAVHLPLSIEAQVEAHTLMLSINNIFSPASGSPMLSPSQDIVLGNYYI
                     THMASGQKGEGRLFRDPAEAITAMEMKTVSVHAKVKVRLPADKKILGENGEEKGPVIE
                     TTPGRCLFNDILPAKMPFYNMVMDKKRLGNVIQDCFDICGSRDTIDLLDNIKELGFKR
                     ATLAGMSFGITDLRIPDEKPAIIEATQKRVDRVMKNYQSGILTEGERYNQIIDAWTHA
                     RVAVTNAMMNAMKNDTRDGKPYLNPIWVMRHSGARGSIDQIQQLAGMRALMAKPSGEI
                     METPIKSNFREGLNVLEYFTSTHGARKGLADTALKTADSGYLTRKLADVAQNVMISQV
                     DCGTLQGITKTTVYKGETIDIPLKQLIIGRTARDNIRNPITDEMIVRENEVITPEAAE
                     KIEAMGLDTIRVRSPLTCESEVGVCSKCYGWDLSTGRLVEEGLAVGIIAAQSIGEPGT
                     QLTMRTFHTGGIASVSLIETDQKSPRDGIVKYVDLNVVETDYDGRPQMISLRRNGEMA
                     LVDEKGRELERFKVPYGGLIQVKDGQSVKRGQTLYAWDPHRVPILAEVPGVVRLMDII
                     EGETMRIEEERKGQKGRPVVIEHKGDKHPQVMIESPEGKILDVHYLPAKARIEVEEGQ
                     NVKAGTLLARLPRSSAGTQDITGGLPRVTEIFEARKPRDPAAMAEISGTVELKTDKRR
                     GKMTIIVRNESGMEKEHHVPRDRHLNFHTGDHVEAGDALTDGPLVPHDILRIKGEEAL
                     QRYLLREVQNVYRAQSVTINDKHIEIILSQMMKKVEIENVGDGPFLPGEVVDKFEFRR
                     ANAELSKSLKITDAGDTDLKVGSVYPKEVVEAANEKAEMLGGNPAKGKKARPATANTL
                     LLGITKASLQSESFISAASFQETTKVLTEASLAGKVDNLHGLKENVILGHLIPAGTAF
                     KPYLDMKVKHLAEPPIPKQLEELREAKEAEAAKDKAVKAALGLE"
     gene            complement(17291..>20155)
                     /gene="rpoB"
                     /locus_tag="PLE88_06740"
     CDS             complement(17291..>20155)
                     /gene="rpoB"
                     /locus_tag="PLE88_06740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOM76196.1"
                     /translation="YQRLRPGNPAQLEKAQTLFNEKFYDINRYRLGSVGRFRLNRKFG
                     TDVDLKEMALRAEDFLHTMKYILNLRSGKGEIDDIDHLGNRRLRTIDELAASEIRKGL
                     LKLRRTVQERMSMKTPTEAIRIADLVNSKSVSSSIEFFFGRGELSQVVDQTNALSQLT
                     HERRLSALGPGGLNRKRAGFEVRDVHISHYGRVCPIETPEGTNIGLIVSLGIFAKVDE
                     YGFLVTPYRKVQDKKLLDEIVYLRADEEMKAVFAPPSMVNPKTNRLLDGMVLARKEGD
                     LTQCDSSEINYVDVSPRQTVGVSAALIPFLEHDDANRALMGSNMQRQAVPLLNTEPPL
                     VGTGLEVEVAKNSSMVVKARKSGTVTAVDAMHIVIDQTDEYPLQKFVGLNERTCLNQR
                     PIVTLGQKVKKGQIIADGGGTSQGCLALGRNVLAAFIPFDGYNFEDAILISERLVEDD
                     VFTSVHIDEFSVEVRETKLGREEFTRDIPNVSEKALRNLDENGIVREGTRVGPDDILV
                     GKVSPKSKTELTPEEKLLHAIFGRAGEDVKNDSLEVPSGFEGVVIKTRHFKRRTAETD
                     EQRKALKDKMRKYEAEMVAKECDVFRRMMKEIHDKLGIEVIDPGTRQKVGASDDNDVI
                     SEQIETFTVDWVKPASARGDVQKIVDQYWPKIIELQDEKKHELERMKHGDELPSGVLE
                     MVKVYVATKRTLSVGDKMAGRHGNKGVIAKIMPVEDMPFLEDGTPVDICLNPLGVPSR
                     MNVGQILETHLGWAAKVLGFDAITPVFAGATEEDIKAVTEEANAHVRKRIQEIEEKKL
                     VPPADELFTQIPPSLKTRIYDGRTGEPFDQDVTIGYIYMMKLHHLVDDKIHARSTGPY
                     SLITQQPLGGKARTGGQRFGEMEVWALEAYGAAYTLQELLTVKSDDIEGRTKIYESMV
                     KGTNTLEAGTPISFDVLCNEIRGLGLNIQLEKKRIGSTPI"
BASE COUNT         4007 a         5936 c         4994 g         5218 t
ORIGIN      
        1 agctcattga gacccacatc ggttccatcc gagaaaacac gctccagctg ggagagattg
       61 accacctcat agcgaacgga aaactgggca ttattaaagc cgcgtttggg cagccgccgg
      121 aacagctgca tctggccgcc ttcaaacgtc agcggcatgg agctgccgga acggctggca
      181 ttgcctttgt ggccgcggcc gcacgttttc ccatgccccg atcctcttcc gcgtccgacg
      241 cgcttgcgct tcggatgtgc tccgacattt tgtgtaattt cattgcttaa catgccgtta
      301 tcaccacctt ttttccactt cgagccctac accgcgcaga gcttcgacag tttctttgtc
      361 acgaagtttc attaagccgt tcatcaccgc tttgaccaca ttcttggccg agcggcttcc
      421 gtacaccttg gtcaggacat tgcgaacgcc ggcatattcc agcacagccc gcacggccga
      481 gccggcgata acgcctgtac cggggctggc cggcagcagg gtgacctgcg tcgcacggca
      541 tttgcctatg accggatgcg gtatcgtgcc ttcgacaatc gggacctgat gcaggttctt
      601 tttggcatcc ttgattgcct tttctaccgc cggcggaact tcattggcct tggcatagcc
      661 gtatccgacc gttccattgc ggtcccctac gacaaccagc gccgagaagc tgaaacgacg
      721 tccgcccttg accacctttg cattccggaa aaccttgacc actgtatctt ccagcggctg
      781 ttccgtctgc atgtctgttc tgatttcttc agccaacttc tatctccgta atcacgtgat
      841 tgatgctgtc tctacagagc caatccggct tggcgagcgg cctctgccag agctttgact
      901 cgaccatgat atttgtagcc gttccgatca aagcatacgg ccttgattcc gacatccaat
      961 gcctgcttgg caatcgccgt gccgacccct ttggccgcat tctgattgcc gccgtaggga
     1021 atcgaatccc gcagggcctt ggcacgggtg ctggccgaaa ccagcgtgat gccggcgaca
     1081 tcgtcgataa tctgggcata aatatgccgg ctggaacgga aaacaaccag acgcggccgt
     1141 tcgggagtgc caaaaatctt ccgacgggag cgccagttgc gtcgtattcg agtctgtttc
     1201 attcgttctt tctgcatatc cgattcctta tacctgacgg acccgcgccg cagcggcgct
     1261 gtccggtcaa ccacaccata ccattatgtg ccgagggcct tgccttcctt gcgaatcaca
     1321 tgttcatccg catagcggat acccttgccc tgatagggtt ccggcgggcg gactttgcga
     1381 atctcggcgg caaactgccc gagaagctgc ttgtcagcgc tggtcagcgt aaacagcgcc
     1441 ggcacatcat tgccgcgggt ggcaggggtt ttgatatcca ccttgacgcc cttgggaatc
     1501 ggcagctcga ccggatgaca atagccgacg ctcagaacaa gcttgccgcc ctgctccttg
     1561 acgttgtagc cggtaccgta aatctgcatg gctttttcaa aacccttgct gacgccgatg
     1621 accatgttgt ttagatgcgc ccgaactgtg ccatgaaggg ctttcttgaa acggctggcg
     1681 gaatccggat tgctgaccac aatctgtttt ccgtccaccg ttacctgaat gtccggctgg
     1741 atctggcgtt ccagcgttcc gcgggggccg ctgattttaa tcgtgcagcc tttctgctct
     1801 acgctgacgc cggccggaat ggcgattggt tttttcccta tgcgactcat ttagctcacc
     1861 gtacaaatca gttcaccgcc gacattatgc tgacggcatt ttctatcact catcaggccc
     1921 aaattggtgg atacaatggc aatccccaat ccgcccatca cacgcggtaa ttcatccttg
     1981 ccgcggtaca cacggcatcc cggcttgctg acgcgggtta tcgatgtaat cacagggtcg
     2041 ccttccggcg tgtacttcaa ctgtatccgc agcaacccct gatgggcgtc ttcaatccga
     2101 tcaaagtccg taatataccc ttcgtctttg agcacctgcg cgacgccctg acacacttta
     2161 ttcgccttga catagacctg ctgcctgcgc acgcgggcgg catttcgtat ccttgtcagc
     2221 atatccgcaa tcggatcact taaactcatt caacggactc cactaaattc tgtttctcgt
     2281 ttgtcgtctt cctaccagct tgccttcttg acgcccggaa tcttgccttc attggccatc
     2341 ttgcggaagc agatgcggca gattttgaat ttgcgataga ccgcccgcgc ccgcccgcaa
     2401 atctgacagc gggtatacgc acggcttctg tatttgggtt tcttccgggc tttattttca
     2461 agtgccttgg tggacattga ttcactccaa aaacgctgta ttttccttag ttggccttaa
     2521 acggcatccc gaacagtttg agcatatcgc gcgcttcatc gtccgtttgg gccgtcgtaa
     2581 caaatgtaat attcataccc tgctgggtct caatccgggc cggatcgatt tccgggaata
     2641 cgctctgctc atccagaccc atcgaataat tgccgtgtcc gtcaaagccg ttggggttga
     2701 gcccgcgaaa atccttgacc cgcggaatcg ccagattaat caggcggtcc ataaattcat
     2761 acatccggtc tctgcgaagc gttactttca ggccggtctt gtccccttgg cggaccttaa
     2821 aattcgacac gctcttcttg gctgcacaaa tcagcggctt ctggccagta attaccgtca
     2881 aatccttgat ggcattttcc aggtatttct tgtcctgaac cgcctttcca acacccatcg
     2941 aaacaacgat cttctgcagc cggggaacag ccatcgggct gctgtaggca tatttctcac
     3001 gcagcgccgg aacaatcttg cttttgtaca aatctttcaa acgagccata gcttaccttt
     3061 ttgccttgac agaccagttt atttcttcgc ggcccgcgtg acgatcccga tttcggttcc
     3121 gtctgcggca acgcgctgtt tattgccttt ttcatcgact gtaaaacgca cgcgggttcc
     3181 ctttgtgccc ttgggattca ccggcagaac attgctgata tgaatcggac gctctacccg
     3241 gatgcggccg ccctgtggat ttttctgcga gggacgcaca tgcttgtacg ccagattcac
     3301 gccttccacc agcacacgct gtttgtccgg aataacccgc atcaccttgc cggtggcgcc
     3361 tttgctgtcg ccggcaatca ctactaccgt atcacctttt ttaacatgtc gtgccattgt
     3421 attcctcatt cctgctgggc ggcagctgtt agaccacctc gctggccagc gaaacaatct
     3481 tcataaaatt cttttcgcgc agctcccgtg ccactgcgcc gaaaatgcgt gttccaaccg
     3541 ggtttttgtc ggcatcgata atcacagcgg cattgctgtc aaaccgcaca taactgccgt
     3601 ccggccgccg caccggatac ttggtccgga taatcacaca ccgataaacc ttttttctgt
     3661 ccaattggca gttcggcaga tgttttttga tcgccacgca gataatatct ccgacggtag
     3721 cggtcgggcg ggtatatttg cccttatgcg aactgcactt gcccagcacc ttaatgcatt
     3781 gtgccgtctt gacgccgctg ttatcggcga tttccaacat tgtttcctgc tgaatcaatg
     3841 gtagcgccct ttattcaatg actgcttttt tcaatacctt taccagccgc cagctttttg
     3901 ttttgctgat cggtctgcat tccgttattt ccaccacatc gccgacgccg gccaggttgc
     3961 cgggatcatg aacgcccagc ttggtgcggc gccgtatata ttttccgtaa tgcggatgtc
     4021 tgaccttgta atcaatctgg cagacaatgc ttttgttgcc gcttttgctg acaaccaccg
     4081 cctgccgcgt tctcagttca tcttttttcg tttccatgcc cttaccggcc ctttatttga
     4141 tcttgtctga taatggtttt gatccgtgca atatcgcggc gcacattgcg aattgcatgg
     4201 cggttttcca gattttccgt cacggcctga ctgcggagcg tgaacagctg ccgcgccagc
     4261 tcgctgagtt tttcctgccg ttcatccggc ctcatgtctc tgatttccga tggcttcatc
     4321 gctacctctg ctgctcttat ccaagcgaat gtcttcgagt tataaaccgg cacttgacag
     4381 gcatcttgtg cgaaacccgc agcagcgccc gtttggccac atcttcgtcg acgccgccga
     4441 tttcatacag aaccgtgccc ggtctaacaa cagcaaccca gccctccact tcggccttgc
     4501 ccttacccat acgcacttcc aatggcttgg cggtatagga gtggtccggg aaaatacgga
     4561 tatacacttt gccttcgcgc ttgagaaagt gggtcgccgc aatacgtccg gcctcaatct
     4621 gccggccggt aatccagctg gcttccagcg actgcagccc gtattcccca aacgccacaa
     4681 aattcttgcg cgaggccttg cctttatttt tgccccgcat gtgcttgcgg tattttactc
     4741 gtttcggcat caacgccata agctttatcc tttaacttgg cagttattca ttcacttcca
     4801 cattcgaatc ggcggcaccg tcagcacccc agtctgcctc tccctgccgt gaactggacc
     4861 ggccggagcg cttctccgca ccgcctttgc ctgtgcggcg cggcttgctc tggacaacag
     4921 gggttatttc ctgaccgaat ttgcctttat aaatccagac cttgattccg attgcgccgt
     4981 aggtcgtaaa agccgtcgaa accgcgtaat ccacattggc atccagcgtc tgcagcggaa
     5041 tgctgcccat tttctgcacc tcgctgcggg caatttccgc accgcccaga cggccgctgc
     5101 agatgatttt gactcccaag gcaccggcat tcatcgcatt ttcacaggtc attttcatca
     5161 cacggcgatg agcagcacgc tttttgagct gctcggcaat gctttcgcca accagtttgg
     5221 catccagagc cggctcctta atctcaatca cattcacgct gattttgcgg tcaatcagag
     5281 cttccagctc ctcgcgaagc ttatccactt cagccccgcg cggcccaatg accatccccg
     5341 gccgggcggt atgcagcgtt actttgactt cattgcgggt tcgggcaatt tcaacctttg
     5401 aaaccgctgc atacggcggc tgacggttga atttcttgtc cacaaactcc cgaatcttat
     5461 aatcttcaac cagaaattcg ccgtaattgg ccttgggagc aaaccagttg ctctgccagg
     5521 gaagcgtaat cccggttctg aacccaattg gagatgtctt ttgacccata acgtgtcctg
     5581 tgtttttaga ttaaccttct gaaactgtta tatgaatatg gctggcctct ttgcgaatcg
     5641 gatgggcgcg gccgcggtct ttcgggaacc actgcttggt gccgagccgg cgtcctgcct
     5701 gatcggcacg ggcttcggaa acaaccagcg catcgacatc ggcttcctgc tcatccgcat
     5761 tggcgatggc gttctgcagc actttcttga ccgtcggcgc tgcccgcttg ttggtaaacg
     5821 tcagcaaatc cagcgcatcc tgcaccggcc ggccggcaat cagctgcgta acaagccgca
     5881 ctttccgcgc tgacatcgga gcatagcgat agctcgcccg ccactcggaa atgtcggcaa
     5941 cttcaacgcc cagcgccgct gcaagccgct cgacatcttc cttgcccggc ttgggagtat
     6001 aaaggccttt cttccacttg cggacagccg acagtgcctc atcacggctc ataccgccgc
     6061 gggccagatg atcggccagc tggctgtcct ggattgcccg ttctctgcaa acttgtgtta
     6121 atttttttcc gctaagcatg ttcaaccttt ttatttactg gcattaccaa cggccggcgg
     6181 tttattttgc tgccccgccg ccggagtgtc ctttgaaggt ccgcgtcatt gaaaactcac
     6241 ccagtttatg accgaccata tcttccgtta caaagacctt gtgaaagatt tttccgttat
     6301 gaaccataaa ggtatagccg acaaattcgg ggataatcgt acaggcacgc gcccatgtct
     6361 tgatgggctc cttgctgccg gtatctgcct gcttgcgcac cttcaggtac agcttttcgt
     6421 ccacataagg gccttttttt gtcgaacgtc ccattcagat tcctcgtttg taattccctg
     6481 tattagtcct gtttactgtg ccgtcttaaa gaaccagctg aaccccgcgg ttgctcttgc
     6541 agcggcgaat aatgcgcttg ctgctgacct tgcgggggtt gcgggtcttg ccgcccttgg
     6601 ctggaacgcc tgtcggcgaa caaggatgac ggccgccgtt ggcccggcct tcaccgccgc
     6661 cgagcggatg ggccaccgga ttcatcgcct tgccgcggac atgaggtctc cgccccatat
     6721 accgcttgcg gccggctttg ccgattttga cattctgata atcgccattg ctgagctgac
     6781 cgatcgtcgc ccggcactct ttgcgaacca tccgcatttc gccgctgggc agcaccaccg
     6841 tcgcccaatc gccttctttg gccatcagac gggctacgcc gccggctgtg cgaaccagtt
     6901 ttccgccttg accggcattc atttcaatat tgtgtatttc gacgcctacc ggcactgccg
     6961 ccagcggcat tgcattgccg atgcgcggct ccaccgccgg cccgctttcc accgtcatcc
     7021 ctacccgaat gccctccgga gccaaaatat agcgtttttc gccgtcggca tattgaacca
     7081 gagaaataaa gcagttgcgg ttcggatcgt attcgatgga ctgcaccgtt gcgggaatat
     7141 tgtccttgtt ccgcttgaaa tcaatgatgc ggtaaatccg gcgagcgccg ccgccgcgaa
     7201 accgcacggt aatttcgccg gtatgattcc gcccgcccgt tttgcgaatg cgtctgcaca
     7261 gcgacttttc gggcttgtct gttgtcagga cggccttgta gtcaattacg gacgcatttc
     7321 gccgtccatt gcttgtcggt ttataaattc gtatcgccat aagttctgcc tgcctttggt
     7381 ttcttagaac gtcttgttaa aacaggtcaa tatgataatc ttgatgcagc acgaccaccg
     7441 cctttttcca gcttttggtt gtccctatgt agcggccgcg ccggcggggc ttgcctttga
     7501 cgttggcggt gcggacatcg gtcaccttga cgccgtaaag ccgctcgacc gcattgcgaa
     7561 tctgaatctt gttggctttc ttgttcacct caaagctgta ggcatttttg gcgtttgcaa
     7621 aatgcattcc ctgttcggtc accaacggcc tgataataat gctgtaatcg tccacgtttg
     7681 tttcctgcta tttctaattg ctttttttct tgtccaacag cgacagaaac gcttctttgg
     7741 taaacagaac cttctgcttg cggcagacat cgccggcgtt cagatcgctg actgtcatca
     7801 catccacttt ggggacgttg cgagccgatt tataaagatt cacatcctga cggctgatgg
     7861 tcaccagaca gctgcggtca atctgaaggt tccccagaat acgggcaaac tcacgcgtct
     7921 taggtgcatc aaacgtcaat tcatccacga cgaccagccg gccagcgagc attttggcca
     7981 acagggcgga atcgcaggcc aggcggcgct gcttcttggg catatcgtgg ctgaaatccc
     8041 gtgcggcttt ggcaaacgcc acgccgccgc cgacccgaac cggcgtacgc actgtgccgg
     8101 cacgggcatg gccggtgccc ttctgcttgt agagcttgcg tgttgaacct tcgaccagtc
     8161 cccgattttt gcttgcggcc gtgccgaccc gcttgttggc gtggtacatc acaattgcct
     8221 gcttgagcag gggataccga accgtacccc cgaacagggc ttcatccacc tgcacgcttt
     8281 ccagttcttt gccttcgcga ttatagactt ttacatcaat cattgttcat tccagtcgct
     8341 atccgaatca tttcctttac ttctgcggcc gggtatggct ggagcgcact tccacataac
     8401 tgccggaagg tcccggcacc gctcccttga ccaccagaag gttattttcc tcatcaatcg
     8461 ccaccacgat gttatgcttg ctggttaccc gcttgccgcc catccgaccg cccatgcgct
     8521 tgcctttctt gaggtttccg ccgtggccgc ggtcgctggc atgcgatgca atcgagccgc
     8581 cggcacggtg cttgcgctcg gttccgtgtg aggcggggaa tccgccgaaa tgatgacgct
     8641 tcatcacgcc ggcatagccc tttcccttgc tgatgccgga aacatccaca tacttaacgc
     8701 cggcaaaagc cgatacccgg acttcatccc ccagtccgta gggcggcgta tcagcatcgt
     8761 tcaagcgcca ttcccgaaca aaccgcttgg gcgctgtctg tgctttggcg gcatgcccct
     8821 gcgcaggttt gagtatccga ttctttttga catcatcata ccccagctgc aatgctgcat
     8881 acccgtcggc ggcgggggtt ttgacctgca tcaccttgca gggaccggct tgtattaccg
     8941 taaccgacac catcttgccg gcgtcatcat aaatctgggt catcccaact ttttttccaa
     9001 gcaacatgac cattgtttta acttcctttg tgcgtgctgc actctcgcct gaatgtgttt
     9061 acttgttctg cctgccgctt tctaaatcct tcggactgcc gcaatcaggc cttgattcta
     9121 acaaaaactc ccgtaggaac gaccaatctg ttaagggctt caaccgtacg ggagttggca
     9181 tcataaatat caatcaaccg cttgtgcgtg cgcatttcaa actgctcacg ggactttttg
     9241 tccacgtggg ggctgcgcag cacggtatag cgttcaatgc gcgtcggcag cggaacaggc
     9301 ccgctgacac gggcgttggt tcgccgggcc tgctcaacaa tttctctggc cgagctgtca
     9361 agcgcccgat gatcgtatgc ttccattcga atcctaatgc gttcggtatc tgtcatggtt
     9421 aacccttata ttgaccttca gtatcgccat ccaaacaatc gaaagccggc aaatttacac
     9481 actctcggct ctgaatacaa gaaaatttgt gtaaaaaata ccgcctttct caaaaaaagg
     9541 acgttttttt cttctttcag gcaggaaagg catctattcg ccttccttta atcgaggccc
     9601 atctacaatc gggcgtttgg agccattttg tccggctctt ttgttaatcg ctaccgccgg
     9661 cttgtggccc taactgccat ccgtgtacca tctcattttt ttgaaatgta cagcccaact
     9721 cgatacgcgg gaaatccttt ttcccgctcg ctccggctgg aaaagttgtc ttttagcaaa
     9781 aagatttcag attgtcaata gcccaataaa attttttctg aaatttgttc aggaactttt
     9841 tcataatcga gcggttccat cgtaaaactg ccgcggcctg ctgttgcact gcggatttgc
     9901 cccgaataac cgaacatttc agccaaaggc actttcgcat caatgacccg catattgccg
     9961 tgcaggcggc tgtcaacaat ttcagcccgt ttggctacaa gcccgctttg gactgcgcca
    10021 tagtttgcct ctggaacaat cacctgcacc cgcataatcg gttccagcag gattgggcca
    10081 gctttctgga cggcttcgcg gactgcgata agggcggcct gctcaaaggc aatttcggaa
    10141 gaatcaacgg gatgaaacga cccgtcaatc aatttaactc ttgcgcccac aataggatag
    10201 ccggacagcg ccccgctggc caggccttcc attgcccccc gttcgacggc agggatatag
    10261 tctttcggta tagcaccgcc aataatagca tttatgaatt catttttacg tgaatagcgt
    10321 ccattttcct caaaaaatgg ttccacagtc aatacgacat gaccatattg cccgcgtcct
    10381 cccgtctgcc ggataaactt gccttcggcc tgagctgtct ggctgattgc ctcacggtag
    10441 gcaacgcgcg gttttcctac cctgacattg actcccatat cgcgcaccaa tttatgctcc
    10501 aaaacctcca gatgcagctc tcccattccg ctgatgatgg tctggccagt ttcctggtca
    10561 aatttgaagt caaaggtcgg atcttctctc ttgagcgaaa ccaatgcttc tgacaatttc
    10621 gaacggtccg aggccgagcg cggctcaatc gacatgctga tgactgtctc ggggaacgtg
    10681 atgctttcga gcatgaccgg aaaatgcgta tcgcataatg tatcccccgt taaggtctct
    10741 ttcagtccca ctaccgccac aatatcccct gcctgcgcgg attcgagtat ctggcggctg
    10801 ttggcatgca tttcaaaaat acgggtgata ttttccttgc ggtcccgatt gatattcagc
    10861 acgcgggtgc ctgttttgag agttccctga tacacacgga taaagtaaag atcgccgtgc
    10921 ttgtctgtca taatcttaaa agacagtcct acgaacggct tatggggatc gcagaggacc
    10981 tgcacgggct gtttttgatc gccttttttg tatccgaccg ccggcgggcg ttcaaggggc
    11041 gaggggaaaa acatggcaac ggcatccagc agccgccgca cgcccatatt tttcagcgcc
    11101 gcccccacca gcaccggatg cagctttccg gctattgttc ccttgcgaag tccggctgta
    11161 atccgttcct ccgtcagcgg ctcatcgtgg atataggcgt gcatcagcgc ctcatcaaac
    11221 tctgccgccg cctcaatcat tcgatgccgc atctgctcag ctttttcgac cagagaaggc
    11281 gggatggctt tttcatagat actggctccg gtttcctccg tcttgaaata aacggctttc
    11341 attgtcagca ggtccacaaa tcctgcaaag tcggactcgg cgccgatagg aatctgcaca
    11401 gccaccggat aagccagcag tctgtttcga atgctttcga tgcacatttc gaaatcggcg
    11461 ccgattttgt ccatcttatt gataaaacag atgcagggaa gatgatattt ctgcccctgc
    11521 cgccaaacag tttcgctttg tgcctgcacc ccttcggagg catcaaaaac ggctaccgcc
    11581 ccgtccagaa cgcggaggga gcgctcgact tcggcggtaa aatccacatg gccgggggta
    11641 tcaattaaat tgataatgtg gtttttccac ggacatttgg tcgcggcaga ggtaatggta
    11701 atgccccgtt cctgctcttc ctgcatataa tccatcaccg ctgttccctc gtggacttcg
    11761 ccgattttgt aggttttgcc tgtaaaaaag agaatgcgtt cggtgacggt tgttttgccc
    11821 gcatcgatat gagccattat cccgatattt cgaactttgg ataaatcggc tgccatacgg
    11881 ctatcctgtt attctgcatc tcccatcggc tgcctgcacg ctgctgtcag gcctaaccga
    11941 accacaaacg agcacttcca tcggtatcgc aaaacgataa aaaaactgcc gcggctgttt
    12001 ggttcaaaca aaaaccgcgg cagtcaataa gcgacgtatt accaagcaaa atgggcaaag
    12061 gccttgttcg cctcggccat gcggtgcaca ttttcccgcg ttgtcatagc tgccccttct
    12121 ttattgtagg catccaccag ttccgatgcc agccgttcgg acatgggttt tccttttttg
    12181 ccgcgggcgg ccgccagaat ccagcgaaaa gccagagact gctgccgctt cgggcctacc
    12241 tgcatcggca cttgatagct ggcaccgccg acacgcttgc tgcggacttc cagcagcggc
    12301 ttgacattgt tgaccgccgt ttcgaagact tccagaggcg gcacatcctt gatcttttta
    12361 gccacctgat ccatcgcatc ataaaaaacc ttgtaggcgg cgcttttctt gccgtcccac
    12421 atcaggcaat tcacaaactt ggcgaccagt ttgctgttga acttcggatc gggttttaat
    12481 tgggtgtacg atgctgtgaa tttttttgcc attgcttctg ccctaagggt ctaagtctat
    12541 agttatgatt tcttcgtgcc gtacttgctg cggccctgct tgcgattggc tgtaccggcc
    12601 gtatccagca caccgcggat aatatgatac cgtacgcccg gaaggtctcg aacacgtccc
    12661 ccgcgaatta gcaccgtgct gtgttcctgc aggttgtggt caattccggg aatataggcc
    12721 gttacttcct ttccattggt caggcgcaca cgagcgattt tacgcagcgc cgaattgggc
    12781 tttttgggtg tctgcgtacg cacaatcagg cagacgccgc gcttctgggg gcttccgctg
    12841 atatcggaaa tgcgcttttt gccttttcgg ctgcgaccgt ttcttactaa ctgatttata
    12901 gttggcataa cttctccaga aaatattaga atttttatta ttcaagaccc aatgccgctt
    12961 taacggcctt atctttggcc gcttccgctt ccttggcttc tctcaattcc tcaagctgct
    13021 tgggaatagg cggctcggcc aaatgtttga ctttcatatc caaatagggc ttaaaggccg
    13081 tcccagccgg tatcaggtgg ccaaggatca cattttcctt caaaccatgc aaattgtcta
    13141 ctttacctgc cagcgaggcc tctgtcaaga cttttgtagt ttcctggaaa ctggcggcag
    13201 agataaagct ttccgactgc agtgaggcct tggtaattcc caacagcagg gtgttggccg
    13261 tagccggacg ggccttttta cccttagcag gattgccgcc gagcatttcg gccttttcat
    13321 tggccgcttc aacaacctct ttgggatata cagaaccgac tttcaagtct gtatcgccgg
    13381 catccgtaat cttcaaactc ttggacaatt cggcgttggc gcggcggaat tcgaacttat
    13441 ctacgacttc cccgggcagg aacggaccgt ctccgacatt ttcgatttca accttcttca
    13501 tcatctgaga aaggatgatt tcaatgtgtt tgtcgttaat cgtaacgctc tgggcacggt
    13561 acacattctg gacttcccgg agcaggtatc tctgcaaggc ctcttcaccc ttaatccgca
    13621 gaatatcatg gggtacaaga ggtccatcgg tgagggcatc gcctgcttcg acatggtcgc
    13681 ccgtgtggaa attgaggtgc cggtcgcgcg gaacatgatg ttctttttcc attccagatt
    13741 cattgcggac aataattgtc atttttccgc gccgcttgtc ggttttcaat tcgaccgtac
    13801 cggaaatttc tgccatcgca gcggggtctc tgggttttcg tgcctcaaag atttccgtaa
    13861 cacgcggcaa gccgcctgtg atatcctgcg tgccggcgga acttcgcggc aggcgggcca
    13921 gcagcgttcc ggccttcaca ttctgccctt cttccacctc gatgcgggct ttggcaggaa
    13981 gatagtgcac gtccagaatc ttgccttcgg ggctttcgat cattacctgc gggtgcttgt
    14041 cgcccttatg ttcaatcaca acggggcggc ccttttgtcc cttgcgctcc tcttcgatgc
    14101 gcatcgtctc gccttcgata atatccatca aacggacaac accgggcact tcagccagaa
    14161 tcggcacacg gtgcggatcc caagcataga gggtttgacc gcgcttgacg ctttggccgt
    14221 ccttaacctg aatcaggccg ccgtaaggca ccttgaaccg ttcgagttcc cgtcccttct
    14281 cgtccaccaa agccatttcg ccgtttcggc gcagtgaaat catctgcggt ctgccgtcgt
    14341 agtctgtttc gacaacattc aaatcaacat atttcacaat gccgtcccgc ggcgatttct
    14401 ggtcggtttc aatcagcgat accgaggcga tgccgccagt gtggaatgtt ctcattgtca
    14461 gctgtgtacc cggctcgcca atcgactggg cggcaatgat gccgacggcc agaccttctt
    14521 ctacaagccg gccggtgctc aaatcccagc cgtagcactt gctgcagacg cccacttcac
    14581 tttcacaggt cagcgggctg cggacccgaa ttgtatccag ccccatcgcc tcaatcttct
    14641 cggcggcctc gggagtaatc acctcgttct cgcggacaat catctcatcg gtaatcggat
    14701 tgcggatgtt gtcgcgggcg gtgcgtccga tgatcagctg cttgagcgga atatcaatgg
    14761 tttcgccttt atagacagtc gtcttggtaa tgccctgcag cgtgccgcaa tccacttggc
    14821 tgatcatgac attctgggcg acatcggcca gcttgcgggt caggtagccg gagtcggccg
    14881 tcttcagcgc tgtatcggcc agacccttgc gggcgccgtg ggtggaggtg aagtattcca
    14941 gcacgttgag gccttcgcgg aagttggact taatcggcgt ttccataatc tcgccgctgg
    15001 gcttggccat caaggcacgc atgccggcca gctgctgaat ctggtcgatg ctgccgcggg
    15061 cgccggaatg acgcatgacc caaatcgggt tgagatacgg tttcccgtcg cgggtgtcat
    15121 tcttcatcgc attcatcatc gcgttggtca ccgccacgcg ggcatgggtc caagcgtcga
    15181 taatctggtt gtagcgttca ccctcggtca ggataccgct ctggtagttc ttcatcacgc
    15241 ggtccacccg tttctgggtg gcctcgataa tcgccggctt ttcatccgga atgcgcaggt
    15301 cggtgatgcc gaagctcatt ccggccaaag tagcccgctt aaagcccagc tctttaatat
    15361 tatccagcag gtcaatcgta tcgcggctgc cgcagatgtc aaaacaatcc tgaatcacat
    15421 tgcccagacg cttcttatcc atgaccatgt tataaaacgg catcttggcc ggcagaatat
    15481 cattgaacag gcagcggccc ggtgtggttt cgatgacggg gcctttttct tcgccgtttt
    15541 cgccgagaat ctttttatca gccggcagac gcaccttgac tttggcatgc acgctgacag
    15601 tcttcatttc cattgccgta atcgcttcgg ccgggtcgcg gaacagccgc ccttcgccct
    15661 tctgaccgga tgccatatgg gtaatatagt agttgcccaa gacgatatcc tgcgagggac
    15721 tgagcatggg agaaccgctg gccggagaga aaatattgtt gattgacagc atcagggtat
    15781 gggcttccac ctgcgcctcg atgctcagcg gcagatgcac cgccatctgg tcgccgtcaa
    15841 agtcggcatt aaatccgccg cacaccagcg gatgcagcat aattgcattg ccttctacca
    15901 gcaccggctc aaaggcctga atgcccatcc ggtggagggt cggagcccgg ttgagcagaa
    15961 cggggtgctg atgaatgact tcttcaagga tatcccagac atgctcctcg cggcgttcca
    16021 gcatacgctt ggcgcttttg atggtatcgg ccagaccgcg gtctttaagc ttgcggataa
    16081 taaacggctg gaacagctcc agtgcaatct ttttgggcag gccgcactgg tacagtttca
    16141 gattgggtcc gaccacaatc accgagcggg cggaataatc cacgcgcttg cccagcagat
    16201 tttcgcggaa tcggccctgc ttgcctttaa tcatatccgt cagcgacttg agcggccggt
    16261 tgttgctgcc cagcaccggc cggcggcagc ggctgttgtc cagcagggaa tcgaccgcct
    16321 gctgaagcat ccgcttttcg ttgcggataa tcacttccgg agcattgaga tcgatcagct
    16381 tcttgagacg gttgttgcgg ttgatgatcc ggcggtacag atcgttgagg tcgctggtgg
    16441 caaaattgcc gctttccagc atcaccagcg gacgcagatc cggcggaatc accgggcaga
    16501 catccagaat catccattcc ggcttgtttt cgctggactt gatggcattg accagcttga
    16561 ggcgcttggt caagtccttg attttctgct tgctgttggt ttcgcttatt tcctgccgca
    16621 gttctgcact gagggcctcg agatccagct tctgaaggag ctccttgatg gcttctgcgc
    16681 ccatcgatgc acgaaaactg ttgccatatt tgctcagcga ttcccgatat tcatcttcgg
    16741 tcaggagctg cttgaccttc agaggactct gtccggcatc ggtgacgaca taatcctgaa
    16801 aatagacaat cttttcgatg tcctgagttt tcatccccag aagggtgctc agacggctgg
    16861 gcagtgcctt gaaaaaccag atatgggcca caggagccgc cagattaata tggcccatcc
    16921 gcttgcggcg gacccggctg tgggttacct tcacgccgca gcggtcgcag atgatgccct
    16981 tgaacttggt gcctttgtac ttgccgcagg cgcattccca gtcgcgctcc ggtccgaaaa
    17041 tccgctcgca gaacaatccg tctttttccg gccggtatgt gcggtaattg attgtctcgg
    17101 gcttccgtac ttcgccgaag gaccagctcc gaatatcatt cggactggct aaggcaatct
    17161 tgaccgaacc gtaatcattg atacgatcgt aaattgattc taccataatc gcaacagctc
    17221 cgatttaacg ttcacattcc ctgtttgcca caggtattta taacgtttcc aaaaaagtga
    17281 acctgtttgc ctaaatcggc gtcgagccga ttcgcttctt ctccagctgg atattcaatc
    17341 caagccctcg aatttcgttg cacagcacat caaaggaaat cggcgtgccg gcctcgagag
    17401 tattggttcc tttgaccatc gattcgtaaa tcttggtgcg gccttcgatg tcatcgctct
    17461 taaccgtcag cagttcctgc agggtatagg cagcgccgta ggcttccagc gcccagactt
    17521 ccatttcgcc gaaccgctgg ccgcccgtcc gggccttgcc gcccaagggc tgctgggtaa
    17581 tcagactgta cggaccggtc gaacgggcgt gaatcttgtc gtccaccaag tgatgcagct
    17641 tcatcatata gatgtagccg atggttacat cctgatcaaa cggttcgccc gtgcgtccat
    17701 catagatgcg ggtcttcagc gacggcggaa tctgcgtaaa cagctcatcg gccggcggaa
    17761 ccagtttttt ctcttcgatt tcctgaatcc gcttgcggac atgggcattg gcttcttcgg
    17821 ttacggcctt gatgtcctct tcggttgccc cggcaaagac cggcgtaatg gcatcaaagc
    17881 ccagcacctt ggccgcccat cccagatggg tttccagaat ctggccgaca ttcatacggc
    17941 tgggaacgcc cagcggattc aggcagatat ccaccggtgt gccgtcttca aggaacggca
    18001 tatcctccac cggcataatc ttggcgataa cgcctttatt cccgtgacgg ccggccatct
    18061 tgtcgccgac ggacagcgtc cgcttggtgg ccacatagac cttgaccatc tccagcaccc
    18121 cgctgggcag ttcatcgccg tgcttcatcc gctccagctc atgctttttc tcatcctgca
    18181 gctcgataat cttcggccag tactgatcaa caattttctg cacatcgccg cgggcggagg
    18241 caggcttgac ccaatcgacc gtaaaggttt caatctgctc ggaaatcaca tcgttgtcgt
    18301 cgctggcgcc gactttttgg cgggtgccgg ggtcgatgac ctcgatgccg agcttatcgt
    18361 gaatctcttt catcatccgg cggaacacat cgcattcttt ggcgaccatt tccgcttcat
    18421 acttgcgcat cttgtctttg agggctttgc gctgctcatc ggtctctgct gtacggcgtt
    18481 tgaagtgccg cgtcttgatg accacgcctt caaaaccgct gggcacttcg agcgaatcgt
    18541 ttttgacatc ctcgccggcc cgtccgaata ttgcgtgcag cagcttttct tccggcgtca
    18601 gttccgtctt gctcttgggt gaaaccttgc cgaccagaat atcgtcggga ccgacgcgcg
    18661 tgccttcccg gacgattccg ttttcatcca gattgcggag ggctttttcg ctgacattgg
    18721 gaatatcgcg ggtaaactct tctcgtccca gtttggtctc gcggacttca acactgaatt
    18781 catcgatatg cacactggta aagacatcgt cttccaccag ccgctcgctg atgagaatcg
    18841 catcctcaaa attatatccg tcaaacggga taaatgccgc cagcacattg cgtcccagcg
    18901 ccagacaccc ctgactggtt ccgccgccgt cggcaatgat ctgtcctttc ttgaccttct
    18961 gccccagcgt cacaatcggc cgttgattca ggcatgtccg ctcattgagc ccgacaaatt
    19021 tctggagcgg gtattcatcg gtctggtcaa tgacgatatg catcgcatcc acagctgtaa
    19081 cggtgccaga cttgcgggcc ttgacgacca tgctggagtt tttggctact tcgacttcca
    19141 gccccgtgcc gaccagcggc ggttcggtat taagcagagg aaccgcctga cgctgcatgt
    19201 tggagcccat cagcgcgcgg ttggcatcat cgtgctccaa aaagggtatc agggctgccg
    19261 acacgccgac cgtctgacgc ggcgaaacgt ccacataatt gatttcgctg ctgtcgcact
    19321 gcgtcaggtc gccttccttc cgcgccagca ccattccatc cagcagccgg ttggttttgg
    19381 gatttaccat gctgggcgga gcaaagacgg ccttcatttc ctcgtcagcc cgcagataga
    19441 cgatttcgtc aagcaacttt ttatcctgca ccttgcggta cggcgtcacc aaaaagccgt
    19501 attcatccac cttggcaaaa atgcccaagg agacaatcaa gccgatgttg gtgccttcgg
    19561 gcgtctcaat ggggcagacg cggccgtaat ggctgatgtg cacatcgcgc acttcgaagc
    19621 cggcccgttt gcggttcaga ccgccggggc ccaaggcact caaccgccgt tcgtgggtca
    19681 gctggcttaa ggcattggtc tggtctacaa cctgactcaa ttcgccccgc ccgaagaaaa
    19741 actcgatgct ggaagaaaca ctcttgctgt taaccaaatc ggctatgcgt atagcttcgg
    19801 ttggagtttt catgctcatc cgttcctgca cggtccggcg gagcttgagc agtcctttgc
    19861 ggatttcaga agccgccagc tcgtcaatag tccgcaggcg gcggttgccc aaatgatcga
    19921 tatcgtcgat ttctcccttg ccgctgcgca gattcaggat gtatttcatc gtatgcagga
    19981 aatcttcagc ccgcagcgcc atctctttga ggtcaacatc ggttccgaat ttgcggttga
    20041 gacggaaccg cccgaccgat cccaagcggt agcggttgat atcgtaaaat ttttcattga
    20101 aaagcgtctg ggccttttcc agctgggccg gattgcccgg acgcagccgc tgata
//
LOCUS       DAOTBO010000044        20104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2205_length_20104_cov_5.064692, whole genome shotgun sequence.
ACCESSION   DAOTBO010000044 DAOTBO010000000
VERSION     DAOTBO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 20104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20104
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2205_length_20104_cov_5.064692"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLE88_06745"
     CDS             <1..406
                     /locus_tag="PLE88_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76197.1"
                     /translation="NVIGCIKTTKTAAGFDATAYPLMLYRNEYGTIPVKVDGSSERLD
                     VAAALTDDKAFLTVGVVNATRDTYKLRLNLAGASGTGAAKTWVIANANPLAYNEPGKE
                     PVIAIQQAADTDLGQPITAAPLSITLFKLPLK"
     gene            complement(488..2548)
                     /locus_tag="PLE88_06750"
     CDS             complement(488..2548)
                     /locus_tag="PLE88_06750"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOM76198.1"
                     /translation="MLQHGVVFAATVFLGAFLLFQVQPLLGRYVLPWFGGTPEVWTTC
                     MLFFQLFLLAGYLYAHFLIRIRNPRIQAALHIAGLAGALLFLPILPSDNFKPTPNSNP
                     LLQILLICTLTVGLPYLMLASASPLIGAWFAKTFPAKNPYRLYALSNAGSLLGLMGFP
                     FVFEPFFTRLQTVYLWSAAFAVFAVLFAACMLLAGRAAPVQPAAKSCPSGSKRQPERR
                     WLWLALPAAAVVELLAVTNKITQDITVIPFLWVLPLSIYLLSFIICFDHQRWYKRSVF
                     LLLFILGMGGIMYVHLFEEGMSAYTLVGIYVFMLLGCCMVCHGELYRLRPAQEHLTVY
                     YLMIAAGGAMGGFFVAVICPLIFNVYIELYLGLLVCACLLLLADQSLSAAGARRRPFW
                     IAAVFVVGLLGIFLMGQRTSDSQRSIENSRNFFGVLGIWEEAWDNPADHKLLMQHGTT
                     FHGLQFQSAQKRHLATAYYSPDSGIGLLLRHLPKQENRRIGIVGLGVGTLAAYGHPGD
                     TLCFYEINPDVERLARKYFTYLSDCPAQIDIVKGDARLSMEHQPSQNFDVLAIDAFSS
                     DAIPVHLLTAEAMAIYLKHLAPEGVLAFHISTMHLDLHSVIWKLADHFGLKTAWIESY
                     KDDSSGALDSDWILLAQDDRFLGSEAIRLNASPQDRRRKDVLLWTDDHINLLQILKKN
                     PLSR"
     gene            complement(2677..4107)
                     /locus_tag="PLE88_06755"
     CDS             complement(2677..4107)
                     /locus_tag="PLE88_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOM76199.1"
                     /translation="MNKNTIPDNLRRLLEKIGQEHILHFFDELSEGQKKNLIDQISKL
                     ELDKIPLWVEKYIKNEAPVAIPDHFEPAPSYPAAPKTPAMAQKYARAKKRGEDLVRQG
                     RVAGFVVAGGQGTRLGYDGPKGNYPISPVKNKTLFQIFAETLRAASKKYGITIPFYIM
                     TSPLNYAATVDIFESADWYGLSPDNVFIFQQGTLPNFSFDGKILLAAKDQIAASPDGH
                     GGSLKALYASGAVADMKQRGVEYISYWQVDNPLVKLIDPLFIGLHDLDKAEMSSKALV
                     KAGPLEKVGNFCLVDGKVTVIEYSDLPDEQALRRNPDGTLVFELGSIGIHIINRSFIE
                     KLNAGGNFALPFHRAVKKIPHIDGNGTLIQPDKPNGIKLETFVFDALPMAERSIILQT
                     RREEEFAPVKNASGTDSPEVTRRMMIERAANWLQHAGVKVPRKPDGSPDCVLEIAPSF
                     ALEPEDIAAKRGGTPPITAGSEIYLE"
     gene            4561..5547
                     /locus_tag="PLE88_06760"
     CDS             4561..5547
                     /locus_tag="PLE88_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM76200.1"
                     /translation="MRKKGFTLIELLVVIAIIAMLLAILMPALNKVKKIAQRVVCGTN
                     LKGLGTAQTVYANDYDDEYAVQGQSGKHKWDWITGAFDDTAKNWANNGIITIGASLYL
                     LVREADVSPKSFVCPSGNETEYDGKNSMNLDIVQLWDFGNEYKAPGSGYPYQHVSYSY
                     HMPYANGGGKSSLSANGSRSAAFAVMADKNPYFDPKNVEIGSVTQDNFMDHVGPVGAY
                     WDTTTGISESDYRVKRANSASHAKEGQNVLFADGHNAYENTTDVGIKNDQIYVPRPTG
                     ALTELQIRQGNVTIMGSGTSPAAGHEPAIQPQGLEDSYLVNDDDMKEKPKTS"
     gene            complement(5595..6824)
                     /locus_tag="PLE88_06765"
     CDS             complement(5595..6824)
                     /locus_tag="PLE88_06765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM76201.1"
                     /translation="MKIAFVVSRFPTLTESFVVQQIAAMIERNWDAAVWAFAESQQTE
                     IQKEVKEYGLLNKTVYLSMPSAKTALRLKGVFWLVASLLLSPKNASSLLYSFIKDRSF
                     SYQTLLLLYAYLKGKPDVVHAHFGPNAIPFLGLKKTGLNLKLITSFHGYDVSVYTRRQ
                     GEHVYDELFLYGDIFTYNSESTRQKLLNLGCPEGKMIKLPMAVEVEKIPFKERFLNPS
                     DKIRLLSVGRLVEMKGREYALKAAAKLKDKYPLQYDIVGDGPLRPDLEKLAADLGLGS
                     SVKFWGWTSSEALTDLYDQAHLFVHPSITSSDGNQEGQGMVLLEAQACGIPVVATKHG
                     AFPDSVIDGRTGFLVPEKDADALAERLDFLISSPHLWPQMGRLGRRFVQDNFDSKILN
                     NRLEDIYRRLVQAKQSQ"
     gene            6955..8565
                     /locus_tag="PLE88_06770"
     CDS             6955..8565
                     /locus_tag="PLE88_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOM76202.1"
                     /translation="MNEQKRVLIIGLDGFCWGLGEKFLKENWMPALGSFTTGGSFGTL
                     QSVLPYETSPAWVSFQTGCFPPKTGIFSFHTFDSQTSRIRLNSSGDIRVPTLWELLSS
                     QGKKTICVNMPLTSPPPAIDGIIIPGLLCPQLNSQTVHPSAIYGRFIKPRKDYQIVDN
                     TYRNTVSAFAEQSIRTEQVRCEVALELMDGYDWDVFCLQIQSTDLFQHRFWWALDSGA
                     NGYNQEDYRQAAAFYKCCDDIISRLIDKAGSNVRVILVSDHGFTAQRWTLAVNRWLLE
                     KGYLVLLEKEKSGWQRAKEKSVFLKYFAQQAGRTKGFFVKAAERFFPPKTPPFSEIEL
                     LHLRQSIDFMRSKAFYLGSVGGVIYIHPSVPHRDDFRNRLIEELLTDFGPAAQPALVK
                     AVKTCAEEYGDLEGAALLPDLLLEYEPGVSAVINPLAPQLVQSYQNTERQPGTHDRNG
                     LFAAQGENIKKNFRCDCQIVDIVPTVLAMLDIPIPAHIDGRILSEIFEKEPRTAYSGG
                     ISKDSQPTYYSDREQSEVERRLADLGYL"
     gene            complement(8560..10581)
                     /gene="asnB"
                     /locus_tag="PLE88_06775"
     CDS             complement(8560..10581)
                     /gene="asnB"
                     /locus_tag="PLE88_06775"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOM76203.1"
                     /translation="MCGIAGYFNHAKTRTDLSGLYKAVRLIAHRGPDDEGYAVFNMDT
                     QTAQKYCGPDSPAVLKSQMPDIVQHPHFAHHLAFGFRRFSIVDLSEKGHQPFWSPDGS
                     VCLIFNGEIYNYIELQKELLQLGYCFTTACDTEVLLAGYLTWGMDVLKFCNGPIAMVC
                     YDRRKNTLLMARDRLGKAPLYYAVHQGTLYWASEIKSILSLTGHSAFSINEQAVYDYL
                     NYGWRDLDNTTFWNGVCTLPAAHWTEIDVASQPSYDQINASLHRYWDFPPQRLHPGQI
                     PFREAVGRFRELFTDAVRIRARADAKVAFSLSGGLDSSSITAAAAHIADQSFRTYSIK
                     FPGQDCDEEPFARKVYELYPDKIDYHIYTPANEDFWQVASDFIWLQEEPFHWPSAELF
                     QAYFRHARRQDYKVILIGTGGDELLAGYNDYFFPLLMYWRKNSRFLPLAANLFLKEDI
                     WPKYCIRQRIKIFNALFHNRFDALRRYIPVSFFNAGGQEVAAGYLRGPLASGSYQKHK
                     QIAPTDFQALAVGYMSNWLMNYWLRNSNRAHFGVPIESRSPFLDYRLVDFVFTLPPEY
                     MVHNGWTKYILRKAMAQWLPSEVSWRRIKQGMPFNTLPWFNHAKPIVEALLRNTAQNP
                     YLDTAQLFNDYDRLLRENPYFLWRGINFCLWWKRVILLSPLQIPAGTTS"
     gene            complement(10609..11580)
                     /locus_tag="PLE88_06780"
     CDS             complement(10609..11580)
                     /locus_tag="PLE88_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76204.1"
                     /translation="MTTLLNEPWDYLIVLDACRFDYFEQMWKNYLPAGGQLKRIESPG
                     SSTDEWRDNLFGGFYDNIVYITANPQINMSMPVYGYCAKEHFKFVHELWKDSWDSHWG
                     TVLPESVTRRALEIIAQYPAGMRFVIHYLQPHAPYIHVPYDRPSEFEAFNRFLLNPNQ
                     PDPSRPVRSLLFGRLLPLFRRLYRRTNQPEWILRKLLWIPPRGPMEIAWRTLGTAGLK
                     EAYKNNLKAVLEQVRILAQSLPGTLVITSDHGELLGEEGQFAHPRGSQHPILRNVPWL
                     VIKTNTVRCPSEAGPPDQPKGSAAPQTETVKNEDEIIQRLRALGYYD"
     gene            complement(11561..13492)
                     /locus_tag="PLE88_06785"
     CDS             complement(11561..13492)
                     /locus_tag="PLE88_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76205.1"
                     /translation="MLNPEAQRYTTHLSLSQLISDAAGGRLQAEYADENLIRCRFCRQ
                     ICNTPVLGTIDYLPHFGIFGVYISHRDQNNTLGIDFDFPGIRQAAGGLFLSEDAHLLI
                     WTTDKAKLVIRDSSRIKDIQEQEIPVKTDNGRVTLQVPAYSADSTELALYVFTEQAAD
                     FLEQIRCLSEIEKRRITRAHWFYYSDWKDFWKYLLGGRFYNLRSPYPFESQLIHSTFY
                     FYFGYLHQQTQKPLYAFLAFYVAYLTLLSLPEDDCWRHGIWQEQDSHYVHWIAGVMVF
                     TDFYKRCRLDIFLDKSRRILDRCIALSDHCRDDTRWYLHDSLEAGGMLHLSPYKNHFA
                     SAAFGKSANCTLTLNTHISTLLAFKMFYDNTGDARYEDAYRKGFKRLGHVLQQRPASG
                     RYAIVYGLRDLLTRALVGKNRIFLQSIIARYDHLLGQHILPRLKKKHPRLAMPNGFIE
                     RDLTFSFYNQFYHLLNTEHLLMIYSIEPSQWLKKLISRSVRYSLASKMVLYLARSDEL
                     ALTFLGVLLQYGTQIDIGGLKSLEQYLLYFVGKGLALPAKILACPFISDCQSDIRTSN
                     PKILVLKADKPLLNAAGLIVNLSAEKQHFTIESRHQTVYYLEDWNGRQQELSAAVSLA
                     GLSSLKLCIRKMPNDNTAE"
     gene            complement(13524..15053)
                     /locus_tag="PLE88_06790"
     CDS             complement(13524..15053)
                     /locus_tag="PLE88_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOM76206.1"
                     /translation="MEQNAQTTKSEPDLYYRSLKGGYWIISSRIAVNILGFIKSLFLF
                     NYFLLNDLGIISAAVMMMEIMSTFTQTGFESALIQKKEDIRDYLDTAWTAGIFKGIFL
                     FAVLFSAAPLLAMFRIPQEKVPLAIWAFRAMSICFLLQGFHNIGAVYFSKNLDFHKTF
                     ILSLASSLADIVLSIALVLVFRSIWGVIAARLVSGAVACIGTYVLSSYRPRLHFNPAK
                     AGELWKYGRWIFGQNILGYLLENGDDFFVWFYLGIQPLALYRAAYQFSNAPFMQINSF
                     FHQVLFPAFSKLQDDPERLRQAYLKVCRVASAVAIPSCLLIFSLGPDFVRLFLPPRLL
                     PMVPVLQILAFKGLLKSLSSVRVPFFQAVGKPNVLLLLMWLRVSVLCLTIYFCTRAWG
                     IVGTAVSVTLVNILVNPVALLLMRMQYQYKIGEVLKQSLLAWCAGALMTLLVLGIKQT
                     ACPDANYICFFGLLIVGALSYLLILIGLDRYKSYGYRDMLREQYILLCKAMQKSRKMF
                     M"
     gene            15659..16213
                     /locus_tag="PLE88_06795"
     CDS             15659..16213
                     /locus_tag="PLE88_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76207.1"
                     /translation="MDSGEQDKQTRDRKAGEFVCLLTSQQARIYAYILSLVPNFSDAD
                     DILQDTTKIMWERFDDFTPGTDFLAWAKKIAYFLIMKYYRDIQKKNTAFYDEQLIRKL
                     DADSQALSVSFKDYHIHLRDCLKKLKQKDRKLLRLRYYENQKVREMASRFGCSIQYVY
                     RNISRIHQFLLACIQKQQMLKDLT"
     gene            16213..17895
                     /locus_tag="PLE88_06800"
     CDS             16213..17895
                     /locus_tag="PLE88_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76208.1"
                     /translation="MPLNAIQKKEIMDLLILSLEGEADSRQLQRLKSLLNDSPEARDY
                     YLRGIVAAECIRKIDWEALDLVNAAGREDFLNAGLWKALADEEIKAPSVQVAAAEEPA
                     VLIQKVQRQKVVRKISKSSIVSLAAAAAAIAFVVLFAHFAPPKGGMEVAVLSDSLNAR
                     WADASAHLKSGMRLTAGGGPWALQEGAAEVIFDNDARIVLEGPCEFDILTSDQILLNY
                     GYLYAAVPRGAIGFTVNTPSARIIDLGTEFGVQADGGGDTCLHVLKGRTALIAGSKSD
                     KQSIEVGAGSAKKVSADLQTVTDIPCNRSLFVRAIDSQTHFAWKGQTSLDLADIAGGG
                     NGLGTGRINMAIDPISGNPVKAEPMPELRTAANAYHPVVSSPLIDGVFVPNGQTQQIV
                     SSQGHVFRECPTSSGACYGNITYAIRILDSQAIPNAGASERWNAHCLLMHANMGITYD
                     LQAIRALLPDIKIVRFQTKFGIEKEAIRPNASNADFWILIDGQIRHQKIQVKEKTLFS
                     ADIELSDKDRFLTLATTDGQDPEGRILDNLMLTTIDSDWCMFVDPVLILEAK"
     gene            18040..18699
                     /locus_tag="PLE88_06805"
     CDS             18040..18699
                     /locus_tag="PLE88_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76209.1"
                     /translation="MKRTALILMCMCTAASYGVVIYDNPAGGTIWAWNWGDNGMGHTA
                     AEVNYGGNIVIQHTGVVNNTTGGNVNYRYGSKWDITVSGNSSADPADYTIAFDLRNVS
                     GSWDPIPLNLAVLTKESGGDQGHGYATMNVAQAAGWVHYEFNLANWANNWWQGAAWNL
                     TNPSWSLEVGMPWPGISTPTGTSFTQIWEMDNLKITMIPEPASLILLGLGALSVVKKR
                     N"
     gene            18809..>20104
                     /locus_tag="PLE88_06810"
     CDS             18809..>20104
                     /locus_tag="PLE88_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM76210.1"
                     /translation="MKKIVSAIAIAAFLCAASAEAVVIYQNDYNSETPGGGVPGSWNW
                     TNGAVTTHTAVYADIGQNMVVRHTGIVNNSGTAAQNSRYGSKWNITVSGNTSADPADY
                     TIELDLRNVSGNWNPIPLEVWVLTLNPAAGTGTYGHGFPTANLTQADGWVHLKFNLGE
                     YTKNWWEGTNWDLTNSTWSLEIGMPYPGQSVAVGQSWTQVWEMDNLKITMGADTLPYE
                     PSVTPVNADGTVGKPVNNNAQVEVTLHFKAGGDPSVETSFPVNPAILGHYIYLSNGNP
                     NDPNVALLDYVLQVHNPDPNLTSPDVSYGPITLTKDTTYYWQVEEAINDGTGNPYPAG
                     DPNNLLGPLWSFVTTAGSAMIDAVSPELTVVGAGTNAVLTVSGSNIETYQWYKIGSPD
                     VLLTNGSKYAGADTAVLTIKDVQLADEGYYYCIGSNGIPT"
BASE COUNT         5117 a         4796 c         5492 g         4699 t
ORIGIN      
        1 caatgtgatc ggctgcatca agaccaccaa gacagcggct ggctttgatg caacggccta
       61 tccgctgatg ctgtaccgca acgaatacgg gacgataccg gtcaaggtgg acggttccag
      121 cgagcgtctg gatgtagccg ccgcgctgac cgacgacaag gcattcctga ccgttggcgt
      181 ggtcaatgca acccgggata cctataagct gcggctgaat ctggccgggg caagcggcac
      241 aggtgcagcc aagacatggg tgatcgccaa tgccaatccg ctggcctaca acgagcccgg
      301 caaggagccg gtgattgcga ttcagcaggc cgcggatacg gatctcggcc agccgattac
      361 agcggctccg ctgagcatca ctctgttcaa gctgccgctt aaataggttc atttcaggat
      421 agggaataag gcgccgccgg cagatttatg ccggcggctt tttttatggg tgcccccgcc
      481 gtccggctca gcgggacagc ggatttttct tgagaatctg cagcaggttg atatggtcgt
      541 cggtccagag caggacatcc ttgcggcgcc ggtcctgcgg gcttgcgttg agccggatgg
      601 cctcagagcc gaggaagcgg tcatcctgcg ccagaagaat ccagtctgaa tccagcgccc
      661 cggaggagtc gtctttgtag ctttcaatcc aagcggtctt gaggccgaaa tggtcggcca
      721 gcttccagat gaccgaatgc aggtccagat gcatggtaga gatatgaaat gccagaacgc
      781 cttcgggtgc cagatgcttc aggtaaatgg ccatggcttc ggcggtcagc agatggacgg
      841 gtatggcgtc gctgctgaag gcgtcaatcg ccagcacatc gaaattctgg gacggctggt
      901 gctccataga cagtcgggca tcgcctttta cgatgtcgat ctgcgcaggg caatcggaca
      961 gataggtgaa atattttctt gccagacgct ccacatcggg attgatttcg taaaagcaga
     1021 gggtgtcgcc gggatgtccg taagcggcca gcgtgccgac gcccagcccg acaatgccga
     1081 tgcggcggtt ttcctgtttg ggcagatggc gcaggagcag gccgatgccg ctgtcgggac
     1141 tgtaataggc ggtggcaaga tgcctctttt gagccgactg gaactgcaga ccgtgaaaag
     1201 tggttccgtg ctgcatcagg agcttatgat cggctgggtt atcccatgct tcttcccaga
     1261 ttcccaaaac gccgaaaaag ttgcgtgagt tttctatgga gcgctggctg tcggaggtgc
     1321 gctgtcccat cagaaagatg cccagcagcc ccacaacaaa gaccgccgca atccagaacg
     1381 gccgacgccg ggcaccggcg gcggaaagag actgatcggc cagcagcaga agacaggcgc
     1441 agaccagcag cccgagatat aattcaatgt acacattaaa aatcagcggg cagatgacgg
     1501 cgacaaaaaa accgcccatt gccccgccgg cagcaatcat caggtaatag accgtcaggt
     1561 gttcctgcgc cggacggaga cggtagagtt ccccgtggca aaccatgcag cagcccagca
     1621 gcataaagac ataaatgccg accagcgtat aggcggacat cccttcttca aaaaggtgga
     1681 catacattat tccgcccatt ccgagaataa acagcagcag gaataccgac cgcttgtacc
     1741 agcgctgatg gtcaaagcag atgatgaagc tgagcagata gatgctcagg ggaagaaccc
     1801 ataggaaggg aataacggtg atatcctgtg taatcttgtt ggtaacggcc agcagctcca
     1861 cgacagccgc cgccggcagg gccagccaca gccaccgccg ctccggctgg cgtttgctgc
     1921 cggatggaca ggatttagca gcgggctgga cgggtgcggc tctgcctgcc agcagcatac
     1981 aggccgcaaa caggaccgca aaaacggcaa aggcggcaga ccacaaataa accgtctgca
     2041 ggcgggtgaa aaacggctca aaaacgaatg gaaatcccat caatcccagc agcgagccgg
     2101 cgttggacag ggcatagagc cggtacggat ttttagccgg aaaggttttg gcaaaccatg
     2161 ctccaatcaa aggggaagca gaagccagca tcagataggg caggccgacg gtgagggtgc
     2221 agatcagcag gatttgcagc aggggattgc tgttgggtgt cggcttgaaa ttgtcgctgg
     2281 gcaggatggg cagaaacagc agggcgcctg ccagcccggc gatatgcaag gccgcctgaa
     2341 tgcggggatt ccggatgcgg attagaaaat gggcatacag gtaaccagcc agcagaaaca
     2401 gctgaaagaa cagcatgcag gttgtccaga cctccggtgt tccgccgaac cacggcagta
     2461 cataccggcc gagcaggggc tgaacctgaa acagcagaaa tgcccccaaa aagaccgtgg
     2521 cggcaaagac aacaccgtgc tgtagcaatc ctgttttctg cattctgggc agattataaa
     2581 gaaacggccg cagtttcgga ataaaaaagt aaagttgaca gctgcaatat agaaattgcg
     2641 gtcatcacaa aagccgtccg ccttgtgcgg tttgtcttat tcaagataaa tctcgctgcc
     2701 ggcggtaatg gggggtgtac cgccgcgttt ggcggcgatg tcttcgggct caagggcaaa
     2761 gcttggggct atttccagca cgcagtccgg cgagccatcg ggcttgcggg ggaccttgac
     2821 gccggcatgt tggagccagt tggccgcccg ttcaatcatc atccggcggg ttacttcggg
     2881 gctgtcggtg ccggaagcgt ttttgacggg ggcaaattcc tcttcccggc gggtctgcag
     2941 aataatcgac cgctcggcca tcggcagggc gtcaaacacg aaggtttcaa gtttgatgcc
     3001 gttgggtttg tcaggctgaa tgagcgtgcc atttccgtcg atgtggggga tttttttgac
     3061 cgcccgatgg aacggcaggg cgaaattgcc gccggcattg agtttttcga tgaatgagcg
     3121 gttgataatg tgaatcccga tagagcccag ttcgaacaca agcgttccgt cgggatttct
     3181 gcgcagagcc tgctcatccg gcaggtcgct gtattcgatg acggttactt tgccgtcgac
     3241 caagcagaaa ttgcctacct tttccagcgg tccggctttg acaagggcct tggaactcat
     3301 ttcggccttg tctaaatcat gcagtccgat aaaaagcggg tcaatcagct tgactaaggg
     3361 attatccacc tgccagtagc taatatattc gacgccgcgc tgtttcatat cggcaacagc
     3421 ccccgatgcg taaagggctt ttaagctgcc gccgtgtcca tcggggcttg cggcaatctg
     3481 gtctttggca gccaacagga ttttgccgtc gaagctgaaa ttgggcaggg tgccctgctg
     3541 aaagatgaac acattgtccg ggctgagccc gtaccaatcc gccgactcga agatatctac
     3601 tgttgcagca tagttcagcg ggctggtcat aatataaaag gggatggtga tgccgtattt
     3661 tttagatgcg gcacggagtg tttcggcaaa gatttgaaaa agcgttttat tcttgacagg
     3721 gcttatcgga taattgcctt ttgggccgtc gtagccgaga cgggttccct gtccgccggc
     3781 aacgacaaag ccggcaacac ggccttgacg gaccaaatct tcaccgcgtt tcttagcgcg
     3841 ggcatacttt tgagccattg ccggcgtttt tggagcggcc gggtaagaag gggcgggttc
     3901 gaaatggtca ggtattgcga ctggggcctc attttttata tatttttcta cccataatgg
     3961 tattttatca agttccagct tggagatttg gtcaatgagg ttttttttct gtccttccga
     4021 cagttcatcg aagaaatgga gaatatgttc ctgcccgatt ttttcgagta agcgacgcag
     4081 attatctggt atagtatttt tattcataaa aaagtacctt tgctgtaaat cattcgtttt
     4141 ttaatacgag ctgttttgta taatgaaaac aggacatttt ttcaattcag aaattttttt
     4201 aaaaaaagct tgacatcgcc gacaaagaga tatacattgt aaattgaaag cgagtgaaca
     4261 gaggcggact ggattcgcgg tgttcaacag aatattattt ttgccattat tttatttata
     4321 gtttttggtt atcttcctgc gaatccttcc caaatgtcat tccttttcag agcagatgtt
     4381 tagcagcttt gatgcggcct ctgacatatt gagtgcctcc ggtctaacgg taagttgtcc
     4441 ggtaagattt acaaaacaaa tggaaaggcc ctgcgttggg gcaaaaacgg cattattgat
     4501 gctttggaaa aggccggaag gcagttgtgg agtaaatctt gcctgaaagg agatctaaca
     4561 atgagaaaga aaggttttac actgattgag ctgctggtgg ttatcgccat catcgccatg
     4621 cttttggcga ttttgatgcc ggccttgaac aaggtcaaga agatcgccca gcgcgtcgtt
     4681 tgcgggacca acctcaaggg tcttggcacc gcacaaaccg tttatgccaa cgactatgat
     4741 gatgagtatg ctgttcaggg gcagtccggc aaacataagt gggattggat tacaggcgcc
     4801 tttgatgaca ctgccaagaa ctgggcaaac aacggcataa ttactattgg tgcaagcttg
     4861 tacctgctgg ttcgtgaagc cgatgtcagc ccgaagtcct ttgtttgccc gtccggcaat
     4921 gaaacagagt acgacggcaa gaactcgatg aacctcgata tcgttcaact gtgggacttc
     4981 ggcaacgagt ataaagcccc tggttcgggc tatccgtatc agcatgtcag ctattcttat
     5041 catatgcctt atgccaacgg cggcggcaag agctctctgt cggccaatgg aagccgctct
     5101 gctgcatttg ctgtaatggc agataagaat ccgtattttg acccgaagaa tgtcgaaatt
     5161 ggaagcgtaa cccaagacaa ctttatggac catgtaggtc cggtcggcgc ctactgggat
     5221 accacaaccg gtatttcgga atccgattac agggtcaagc gagcgaattc tgcttcccat
     5281 gccaaagagg gccaaaacgt gctgtttgct gacggccaca acgcctatga aaacaccacg
     5341 gatgtcggca tcaagaatga ccagatttat gttccgcggc ccacaggcgc ccttactgaa
     5401 cttcagattc gtcagggcaa tgtaacaatt atgggcagcg gcaccagccc ggcagcagga
     5461 cacgaacctg cgattcagcc gcagggcttg gaagattcct atcttgttaa cgacgatgat
     5521 atgaaggaaa agccgaagac aagctgagat ttcgtgcagt ccaaaagccc ttctcctgac
     5581 agagaagggc ttttttattg ggattgcttt gcctgtacca agcgtcggta gatgtcttcc
     5641 agccgattgt tcaggatttt tgaatcaaaa ttgtcttgta caaacctgcg tcccaaacgc
     5701 cccatctgcg gccataagtg agggctgctg attagaaaat ctaatctttc tgccagtgca
     5761 tcagcatcct tttcgggaac taaaaatcct gttcggccgt ctatcacgct gtcagggaaa
     5821 gccccgtgtt ttgtagcaac aaccggtatt ccgcaggcct gtgcctccaa taaaaccatg
     5881 ccttgtcctt cctgattgcc gtcggaggag gtgatgctgg ggtgaacaaa gagatgggct
     5941 tgatcgtaaa ggtccgttaa tgcttcgctg ctggtccagc cccagaattt gacgctgctg
     6001 ccgaggccca aatcggcggc caatttttcc agatcgggcc gcagagggcc gtccccgaca
     6061 atgtcatact ggagggggta tttgtctttc aattttgcag cagctttgag tgcatattcc
     6121 cgccccttca tttccaccag ccggcctaca ctgagcagcc gtattttgtc tgacggattt
     6181 aaaaaccgtt ctttaaacgg aattttttca acctctacag ccatcggcag cttaatcatt
     6241 tttccttctg ggcagcccaa attcagcagt ttctgccttg ttgactcgct gttgtaagta
     6301 aatatgtctc cgtatagaaa cagttcgtca tagacatgct cgccctgccg gcgggtataa
     6361 actgatacat cgtagccgtg aaaagaggta atcagcttta gattgagtcc tgtttttttt
     6421 aatcccagaa atgggatggc gttgggaccg aaatgagcat ggacaacatc gggttttccc
     6481 ttcagatatg catacagcag taaaagggtt tgataggaaa aacttctatc tttgatgaag
     6541 gaataaagca gggaagaggc atttttgggg gataacaaaa gactggctac aagccaaaag
     6601 actcccttga gccgcaacgc tgttttggcc gagggcatag acagataaac tgttttattt
     6661 aaaaggccgt attctttgac ttctttttgt atttctgtct gctgggattc tgcaaaagcc
     6721 caaaccgccg catcccagtt tcgctcaatc atcgccgcaa tttgctgaac cacgaaactt
     6781 tcggtcagag tgggaaaacg acttacgaca aatgcgattt tcatttttaa tttaccagtc
     6841 tattcatccg gctgctattt gacgattatt tttgttgatt atatcaggga atagcataat
     6901 atttaaggtt cgtcaatctt aataagagta aagatcttgt gcgaaaacgg cttaatgaac
     6961 gaacaaaaac gtgttctgat aatcggttta gacggttttt gctggggctt gggggagaaa
     7021 ttcttaaaag aaaactggat gccggctctg gggtcgttca cgacgggcgg gagttttggg
     7081 actttacaaa gtgttctgcc gtatgaaaca agtcctgcat gggtgagctt tcagacgggc
     7141 tgttttccgc cgaaaacggg catcttttca tttcatacat ttgattcaca aaccagccgg
     7201 attcgattaa acagcagcgg ggatattcgt gtgccgacac tgtgggagct gctgagcagt
     7261 caaggcaaga aaaccatctg cgtcaatatg cctctaacca gcccgccccc tgcgattgac
     7321 ggcatcatta ttcccggtct tttatgcccg cagttgaaca gtcagacagt tcatccatcg
     7381 gctatttacg gccgctttat caagccccgt aaggactacc agattgtgga taatacctat
     7441 cggaacacag tgagcgcatt tgctgagcag tctatccgaa ccgagcaggt ccgctgtgaa
     7501 gttgcgctgg aattgatgga cgggtatgac tgggatgttt tttgcctgca gattcagagt
     7561 accgatttat ttcagcaccg attctggtgg gcgctggatt ccggagccaa tggatacaat
     7621 caggaggatt accggcaggc ggcggctttt tataagtgct gcgatgatat tatcagccgg
     7681 ctgattgaca aagccggttc caatgtccgt gtgatactgg tgtcggatca tggctttacg
     7741 gcccagcgct ggacgctggc ggttaatcgc tggctgcttg aaaagggcta tctggtgctg
     7801 ttggagaaag aaaaaagcgg ctggcagagg gccaaagaaa agagtgtttt cctgaaatat
     7861 tttgctcagc aggccggacg gacaaaagga ttctttgtga aagccgctga aaggttcttt
     7921 ccccccaaaa cgccgccgtt cagtgaaatt gaactgcttc atcttcgcca gtctattgat
     7981 tttatgcgtt ccaaggcgtt ttatctcggc tcggtggggg gcgttatcta tatccatcct
     8041 tctgtgcccc atcgcgacga tttcagaaac cggctaatcg aggaactgct gacggatttt
     8101 ggtccggcgg cacagccggc tcttgtcaaa gcggtcaaaa cgtgcgctga ggaatacgga
     8161 gatttagagg gggctgcctt gctgccggat ttgctcttgg agtatgagcc gggtgtctcg
     8221 gctgtcatca atccgcttgc accccagctt gttcagtcct atcagaacac cgaaaggcag
     8281 ccgggtacgc acgaccgaaa cggcctgttt gccgcacagg gtgagaatat aaagaaaaat
     8341 ttccgatgcg attgtcagat tgtcgatatt gtcccgaccg tgctggccat gctggatatt
     8401 cctatccctg cgcatatcga tggccggatt ctaagcgaaa tctttgaaaa agaaccccgg
     8461 acggcataca gcggcggcat ttcgaaagac agccagccga cctactattc ggacagggag
     8521 caatccgagg ttgaaagacg tttggcggat ttggggtatt tatgatgttg tgccggcggg
     8581 gatttgcaga ggggatagaa gaataaccct tttccaccag aggcagaaat tgatgccgcg
     8641 ccacaggaaa tagggatttt ccctgagcaa tcggtcataa tcgttgaaca gttgggccgt
     8701 atccagatag ggattttggg ctgtatttct caggagtgcc tccacaatcg gcttggcatg
     8761 gttaaaccac ggcagggtgt taaagggcat tccctgcttg atgcgtctcc agcttacctc
     8821 agagggcagc cactgtgcca tcgcttttcg cagaatgtat ttggtccatc cgttatgcac
     8881 catatattcc ggcggaaggg taaaaacaaa atcaaccagt cggtaatcca aaaaaggaga
     8941 gcgggattcg ataggcaccc cgaagtgcgc ccgattggag ttgcgcagcc aatagttcat
     9001 cagccagtta gacatataac cgacagccag tgcctgaaaa tccgtcgggg caatttgttt
     9061 gtgtttctga taagagccgc tggccagcgg tccgcgaaga tagccggcgg cgacttcctg
     9121 accgccggca ttgaagaaag aaacagggat atagcgtcgg agagcatcga aacggttgtg
     9181 aaacagggca ttaaaaatct ttatccgctg acggatgcag tatttaggcc atatgtcttc
     9241 tttcaaaaaa aggttggctg ccagcggaag gaaccggcta ttttttctcc agtacatcag
     9301 cagtgggaaa aagtagtcgt tgtatccggc cagcagttca tcgccgccgg tgccgatgag
     9361 aataacttta tagtcttgcc ggcgtgcatg gcgaaaatag gcctgaaaca gctcggctga
     9421 aggccagtgg aaaggttctt cctgaagcca gataaaatcg ctggcaacct gccagaaatc
     9481 ttcattggca ggcgtgtaga tgtgatagtc gattttgtcc ggatacaatt catagacctt
     9541 gcgggcaaaa ggttcctcgt cgcagtcttg gccggggaac ttgatggaat aggtgcgaaa
     9601 cgattgatcc gcgatatggg ccgcagcggc ggtgatggag ctggagtcaa gcccgccgct
     9661 cagggagaag gccactttgg catctgcacg tgcgcgaatc cgcacagcat cggtaaacag
     9721 ttccctaaac cggccgaccg cctcgcggaa ggggatttgg ccgggatgca gccgctgggg
     9781 tgggaagtcc cagtagcggt gcaggctggc attgatctgg tcataactgg gctgcgaggc
     9841 cacgtcaatc tctgtccagt gtgccgccgg cagggtgcat accccgttcc agaaggtggt
     9901 gttgtcgagg tcgcgccagc cgtagtttaa ataatcataa accgcctgct cgttgatgga
     9961 aaaagccgaa tgccctgtca gcgagagaat ggacttgatt tccgatgccc agtacaacgt
    10021 tccctgatgg accgcgtaat acagcggagc cttgcccaga cggtcacggg ccatcaacag
    10081 cgtatttttg cgccggtcgt agcaaaccat agcaatcggg ccgttgcaga acttcaaaac
    10141 gtccattccc catgtcagat agccggccag cagcacttcc gtatcgcagg ccgtcgtgaa
    10201 gcaataaccc agctgaagca attccttctg cagttcgatg tagttgtaga tttctccgtt
    10261 aaagatcaga caaaccgagc cgtcggggct ccaaaagggc tgatgcccct tttctgacaa
    10321 gtccacaatc gaaaagcgcc ggaatccgaa tgccagatga tgcgcaaaat ggggatgctg
    10381 aacgatatcg ggcatctgcg atttcagaac cgccggcgaa tcgggcccgc agtatttctg
    10441 ggcggtctgt gtgtccatat taaaaacggc gtacccttca tcatccgggc cgcgatgggc
    10501 aatcagccgc acggccttgt aaagcccgga taaatccgtg cgtgttttcg catgattgaa
    10561 atagccggct atgccgcaca ttgttttgtc cttctctgga ggctggcgtc agtcataata
    10621 tccgagtgca cggagccgct ggataatttc atcctcgttt ttgactgtct ctgtctgcgg
    10681 ggcagcagat cctttcggct ggtccggcgg ccccgcttcg gatggacagc ggacagtgtt
    10741 ggttttaatc accagccagg gaacatttcg caaaatggga tgctgggacc ctcggggatg
    10801 agcaaattgc ccttcttcgc cgagcaattc gccgtggtcg gaggtaatga ccagcgtgcc
    10861 gggcaaagat tgagccagta ttcggacctg ttccagcacg gccttcaggt tgtttttata
    10921 ggcctctttt aagcctgctg tccccagtgt tctccatgca atttccatcg ggcctcgggg
    10981 gggtatccag agcagcttgc ggagaatcca ttcgggctga ttggttcggc ggtagagacg
    11041 cctgaacagc ggcagcaggc ggccgaacag cagtgaccgg acaggccggg acggatccgg
    11101 ctgattggga ttcagaagaa accggttaaa cgcctcgaat tccgaaggcc tgtcataggg
    11161 cacatggata tacggcgcat ggggctggag atagtgaatc acaaaacgca ttccggccgg
    11221 atactgggca ataatctcca aggcccgccg ggttaccgat tccggcagaa cggttcccca
    11281 gtgcgaatcc cagctgtctt tccagagttc atgaacgaat ttaaaatgct ctttggcaca
    11341 gtacccatag accggcatcg acatattgat ttggggattg gcggtaatat agacgatatt
    11401 gtcatagaaa cccccgaaca gattgtccct ccattcgtcg gtcgaggagc cgggtgattc
    11461 aatccgcttc agctgcccgc cggcaggcag atagtttttc cacatctgtt cgaaataatc
    11521 gaaccggcag gcatctaaga caatcaaata atcccacggt tcattcagca gtgttgtcat
    11581 tgggcatttt tcttatgcaa agttttaagc ttgataatcc ggccaagctg acggcagcgg
    11641 atagttcctg ctgtcggccg ttccaatctt caagataata cacagtttga tgccggcttt
    11701 ctattgtaaa atgctgcttt tcggccgaca gattgacaat cagtcccgcg gcgttgagca
    11761 ggggtttgtc ggccttgagc accagaatct tcggattgga ggttctgata tcggactgac
    11821 agtcggagat gaacggacaa gccaagattt tggcgggcaa agccaatccc tttccgacga
    11881 aatacaggag gtattgttcc aaagatttga ggccgcctat gtcgatttgc gtgccgtatt
    11941 gcagcaaaac gccaagaaaa gtcagggcca gctcatcgga gcgggccaaa tacaaaacca
    12001 ttttagaggc caacgaatag cggacacttc ggctgatgag ctttttaagc cattggctgg
    12061 gttcgatgct gtaaatcatc agcagatgct ccgtattgag caggtgatag aactggttgt
    12121 aaaaactgaa tgtaagatcc ctttcgataa atccgttcgg cattgccagc cgcggatgct
    12181 tctttttaag cctcgggagt atatgctgcc caagcagatg gtcatagcgg gcaatgatgg
    12241 actgcaagaa aatcctgttt tttccgacca aggcacgggt taataaatct cgcaggccgt
    12301 agacaattgc atacctgccg gatgccggcc gctgctggag gacatggccc agccgtttga
    12361 atcccttccg gtatgcatcc tcgtatctgg catcgcctgt gttgtcgtaa aacatcttga
    12421 aggccagcag tgtactgata tgggtattta aggttaacgt gcagtttgcg gattttccga
    12481 aagcggcgga tgcgaaatga tttttgtaag gggacagatg cagcatcccg cctgcttcca
    12541 gactgtcgtg aagataccag cgggtatcgt cgcggcaatg gtcagacagg gcaatgcagc
    12601 ggtccaaaat acggcggctt ttgtcgagaa aaatatccag ccggcagcgc ttatagaagt
    12661 ccgtaaatac catcacgccg gcaatccaat ggacatagtg actgtcttgc tcctgccaga
    12721 tgccgtgccg ccagcagtca tcttccggca gacttagaag cgtcaaatag gccacataga
    12781 aggccagaaa ggcgtaaagg ggcttttggg tctgctggtg cagatagcca aaatagaagt
    12841 agaaggtaga atgaatcagc tgggattcaa acggataagg gctgcggaga ttgtaaaagc
    12901 gcccgcccag caggtatttc cagaagtcct tccaatcgga ataatagaac caatgtgcac
    12961 gtgttatccg gcgtttttcg atttcggaaa gacagcggat ttgttccaga aaatcagctg
    13021 cctgttcggt gaagacgtac agcgccagtt ctgtggaatc ggctgaatag gcaggcacct
    13081 gaagggtaac acggccgtta tccgtcttaa ccggaatctc ttgttcctga atgtctttga
    13141 tgcggctgct gtcacggata acaagttttg ctttgtctgt tgtccatatt aacagatgcg
    13201 cgtcttcaga aaggaaaagg ccgccggccg cctgccggat gccgggaaaa tcaaaatcga
    13261 tgcccagcgt attgttttgg tctcgatgag agatataaac gccgaagatg ccaaagtgcg
    13321 gaagataatc aatggtaccc agtaccggtg tattgcaaat ctgccggcag aaacggcagc
    13381 gaattaggtt ttcatccgca tactctgcct gcagccggcc gccggcggca tcagaaatca
    13441 gctgggacaa tgatagatgt gtagtgtagc gctgtgcttc tgggttaagc atggtaggat
    13501 cccgagttat caagaccgaa ggtttacata aacatctttc tgcttttttg cattgccttg
    13561 cacagaagaa tatactgctc acgcagcata tcccggtatc cgtagctttt gtaacgatcc
    13621 aatccgatta aaatcaataa atagctcaag gcgccgacaa tcaggagtcc aaaaaagcaa
    13681 atgtaatttg catcgggaca agccgtctgt ttgatgccca aaaccagcag cgtcatcagc
    13741 gcaccggcac accaagccag cagggattgt ttcagcacct cccctatttt gtactggtat
    13801 tgcatccgca tcaaaagcag cgcaacggga ttaacaagga tattaaccag tgtaacagat
    13861 accgccgtgc cgacaatccc ccaagcccgt gtgcagaagt aaattgtcag gcatagaaca
    13921 gaaacccgaa gccacatcag cagcaggagc acattgggtt ttccaacggc ctgaaagaag
    13981 gggacacgaa ccgaactgag tgatttaagc aaacctttga aagccaatat ttgaagcaca
    14041 ggaaccatcg gaagcagccg tgggggcaga aacagccgca caaaatcagg ccccaagcta
    14101 aagataagca gacaagaggg aattgcgaca gctgacgcaa cgcggcacac cttcaaatag
    14161 gcctgacgca aacgttccgg gtcatcctgc agttttgaaa atgccgggaa gaggacctga
    14221 tgaaagaagc tgttaatctg cataaaaggg gcgtttgaaa actgataggc ggctcggtaa
    14281 agggccagcg gctggatgcc cagataaaac cagacaaaaa agtcgtcgcc gttttccagc
    14341 agatacccaa gaatgttctg tccgaaaatc cagcgtccgt atttccacaa ttcaccggct
    14401 ttggccggat taaaatgcag ccggggccgg taggacgata aaacgtaagt gccgatgcac
    14461 gcaacagccc ccgaaaccag ccgagccgca attacccccc agatactgcg aaaaaccagt
    14521 accaatgcaa ttgacagcac aatatctgcc agcgaggaag ccaaggacaa tataaaggtc
    14581 ttgtgaaaat ccagattttt gctgaagtaa acggccccaa tgttatgaaa accctgaagc
    14641 agaaagcaga tgctcatcgc ccgaaatgcc cagattgcca gcggcacttt ctcctgcggg
    14701 atacgaaaca ttgccagcag gggagcggcc gaaaaaagca ctgcaaagag aaaaatgcct
    14761 ttgaaaatcc ctgccgtcca agcagtgtct aaataatcgc ggatgtcttc ttttttctga
    14821 ataagggcgg attcaaagcc ggtttgggta aatgtggaca ttatttccat catcatcacg
    14881 gccgcggaga tgattcccaa gtcgttgaga agaaaatagt tgaaaagaaa gagcgatttg
    14941 ataaagccca agatgttgac agcaatccgc gagcttatga tccaatagcc gcccttgagg
    15001 gagcggtaat aaagatccgg ttcacttttg gtcgtttgag cgttttgttc catcttttgc
    15061 agccggcaat cctaaatgca atgctcttaa actataccaa tataagctct taaaagtcaa
    15121 ggaattaaga gcatttcaga caaagggggc tgctgatgga aacacaagat acaaaaaaag
    15181 ccgacagctt gactgcctta atggctcgat tttcctgctg ggggcaaaat aacagcaggc
    15241 ttctgcttaa gaatttgctt ttgactgaag cctgtcggaa ctggcggggt atgggggagg
    15301 ttcccgactc attgttcgtg ggcgtgtcag atataattgg ataaggattg gatacggaca
    15361 ataaacagaa gatgacaatc tggacttata atagttgaaa taattgcacg aagcagctaa
    15421 ttcagtaaag aaatctagag attattttag atggcagtaa ttatatcaga caaaaatctg
    15481 cgctcttcag ccggctttgt gcggaagagg cagtttttta aaaaaaaatt ataagccatt
    15541 gcaaataaag gtattagcat cttttttcga agactttttg gtttaaaata taagagattt
    15601 agtcattaaa ttatgttaga aatgcccagc aggcggttaa aaccaaagac ggataattat
    15661 ggacagcgga gaacaggata aacagactcg agatagaaaa gcaggggaat ttgtctgtct
    15721 cctgacgtcc cagcaggcac ggatttatgc ctatattttg tcgctggttc cgaatttcag
    15781 cgatgccgat gatatccttc aggataccac caagattatg tgggagcgtt ttgatgattt
    15841 cacacccggc acggattttt tggcgtgggc taagaaaatc gcctattttc tgattatgaa
    15901 gtattatcgt gatatccaga aaaagaacac ggctttttat gatgaacagc tgatccgaaa
    15961 gctggatgcc gactctcagg ccctgtcggt ttcgttcaag gactaccata ttcatttaag
    16021 agattgtctc aaaaagctta agcagaagga tcgcaaactg ctgcggctgc gctactatga
    16081 gaatcagaaa gtcagagaaa tggcatcgcg attcggctgt tcgattcagt atgtttatcg
    16141 caatatttcc cgcatccatc agtttctgct tgcctgcatt cagaaacagc aaatgctgaa
    16201 ggacctgaca taatgccgct gaatgcgata caaaagaagg aaataatgga cctgctgatt
    16261 ttgtctcttg aaggcgaggc cgacagccgg cagctgcaga ggctcaaaag tcttctcaat
    16321 gattcgccgg aggcacggga ctattattta aggggcattg ttgctgccga atgcatccgc
    16381 aaaatagact gggaagcgct ggatttggta aatgcagccg gtcgtgagga ttttctcaac
    16441 gctgggctgt ggaaggcgtt ggcggatgag gaaataaaag ccccctccgt tcaggtggcg
    16501 gcggctgaag agcctgctgt tctgattcaa aaagtccagc ggcagaaagt cgtacgaaaa
    16561 atcagcaaaa gctccattgt ttcgctggcc gcggccgcag cggcaattgc gtttgtggtt
    16621 ttgtttgcgc attttgcgcc ccccaagggc ggaatggaag tcgctgtgct gtcggacagt
    16681 ctcaatgccc gctgggcgga tgcatctgcc cacctgaaat cgggcatgcg gctgacggcc
    16741 ggcggggggc cgtgggctct gcaggagggg gcagcggagg tgatttttga caacgatgca
    16801 aggattgttt tggaagggcc gtgtgaattt gacattttga ccagcgacca gattctgctg
    16861 aattacggct atttgtatgc ggcggtaccg cggggggcta tcggctttac ggtcaatacg
    16921 ccgtcggccc gcattattga tttgggcacg gagttcggcg tacaggccga tgggggcggc
    16981 gatacctgcc tgcatgtgct gaagggcaga acggccttga ttgccggcag caaatcagac
    17041 aagcagagca tcgaagtcgg cgccggatca gctaaaaaag tatctgcgga tttgcagacg
    17101 gtgaccgaca ttccctgcaa ccggagttta tttgttcggg caattgattc ccaaacgcat
    17161 tttgcatgga aagggcagac ctcgcttgac ttggcggata tagccggagg cggcaatgga
    17221 ctggggactg gaagaatcaa tatggcaatc gacccgataa gcggcaatcc ggtcaaagca
    17281 gaacctatgc cggaactgcg aaccgcagcc aatgcctatc atcccgtcgt ctccagcccc
    17341 ttgattgacg gcgtctttgt tcccaacggc caaacgcagc agattgtcag ttcgcaggga
    17401 catgtctttc gggaatgtcc gacatcaagc ggcgcctgtt acggcaatat cacctatgcg
    17461 atacgaatcc ttgactccca ggcaatcccg aatgccggtg catcggaacg ctggaatgcc
    17521 cattgtctgt tgatgcatgc caatatgggc attacctatg atcttcaggc catccgggct
    17581 ctcctgccgg acattaagat tgttcgcttt cagacaaaat tcggaatcga gaaagaggcc
    17641 atccggccca atgccagcaa tgccgacttc tggatactga ttgacgggca aatccggcat
    17701 caaaaaattc aggtcaaaga gaaaacgctt ttttctgctg atatcgagct ttccgacaaa
    17761 gaccgctttt tgacgcttgc tacaaccgat gggcaggacc ccgaagggcg gattttggac
    17821 aatttgatgc tgacaacgat tgacagcgac tggtgcatgt ttgttgatcc ggttctgatt
    17881 ctggaagcga agtaagccat tatgaagaaa aggaggtgat cgacaaggca gtatgtgaat
    17941 aaaaaagttc gatttgtatc aggcaacagg attgtgcgga acggaatctg aaaaagaaac
    18001 caagcaaata gaataaacat tttattggag gaccgaaaga tgaaaagaac ggcattaatt
    18061 ttgatgtgca tgtgtactgc agcaagttat ggtgttgtta tctatgataa tccggccgga
    18121 ggtaccatat gggcttggaa ttggggtgat aatggaatgg ggcatacggc agccgaagtg
    18181 aattatggcg gcaatatcgt tattcagcat acgggtgtcg tcaataacac taccggcgga
    18241 aatgtaaact atcgatacgg ttccaagtgg gacatcacgg tcagcggaaa cagcagcgcc
    18301 gatccggcgg attataccat cgcgtttgat ctgcgcaatg tttcgggcag ctgggatccg
    18361 atcccgctca atcttgccgt gctgaccaaa gagtccggcg gcgaccaggg ccatgggtat
    18421 gcaacgatga atgtggctca agcggccggc tgggttcatt acgaattcaa tctggccaac
    18481 tgggccaata actggtggca gggcgctgca tggaatttaa caaatcccag ctggtccctt
    18541 gaggtcggca tgccgtggcc gggcatctct actcccaccg gcacatcttt tacgcagatc
    18601 tgggagatgg ataatctgaa aattacgatg attccggaac ccgcatcgct gattctgctg
    18661 gggctgggtg ctctgtctgt tgtgaaaaaa agaaattagg atagtggggt ctctcttgtc
    18721 tgcagaggca tttccttgtg aatgaggatg cggacaggca gcggccctgt aaaccgtagg
    18781 caacgagaaa caaatggagg cagtgattat gaaaaaaata gtttcagcta ttgcaatcgc
    18841 agcttttctg tgtgcggctt ctgcagaggc ggtagttatt tatcagaatg attacaacag
    18901 cgagacgccg ggcggcgggg ttccgggaag ctggaactgg accaacggcg cggttacgac
    18961 gcatacggct gtctatgccg acatcggcca aaatatggtt gttcgccata cagggattgt
    19021 gaataattca ggcaccgccg cgcagaacag ccgatacggc tcgaaatgga acatcacggt
    19081 gtccggaaac accagcgccg atccggcgga ttatacgatt gagctggacc tgcgcaatgt
    19141 ctcgggcaac tggaatccga ttcccttgga agtgtgggtt ctgacgctca atcctgctgc
    19201 cggcaccgga acctatggac atggttttcc gactgccaat ctaacccagg ccgatggatg
    19261 ggttcatctg aagttcaatc tgggcgaata caccaagaac tggtgggaag gaaccaactg
    19321 ggatttgacc aattcgacct ggtcccttga aatcgggatg ccgtacccgg gtcagtcagt
    19381 ggctgtaggg cagtcctgga cccaagtgtg ggaaatggat aatctgaaaa ttacgatggg
    19441 agccgataca ctgccttacg aaccgtcggt tactcctgtc aatgcagacg gcacggtggg
    19501 caaaccggtc aataataatg cacaggtaga ggtaacgctc catttcaaag cgggcggcga
    19561 tccgagtgtt gagacgagtt tccccgtgaa tccggcgatt ttgggccatt acatctatct
    19621 gtcaaacggc aatcccaacg atccgaatgt ggcgctgctg gattatgtcc tgcaggttca
    19681 caatcccgac cccaacctga ccagtccgga tgtttcgtac ggtcccatca cacttacaaa
    19741 ggatacgacc tactattggc aggttgagga agccatcaac gatggaacgg gcaatccgta
    19801 cccggccggc gacccgaaca atctcctggg accgctgtgg tcatttgtta caacagcagg
    19861 ctcggcgatg attgatgcgg tcagccccga attgaccgtg gtgggtgcag gtaccaatgc
    19921 ggttttgacg gtcagcggaa gcaatattga aacctatcag tggtataaaa tcgggtctcc
    19981 ggatgttttg ctgaccaacg ggagcaaata tgcgggtgcc gataccgccg tgctgacgat
    20041 taaagatgtt cagctggccg atgaaggcta ctactactgc atcggcagca acggaattcc
    20101 cacg
//
LOCUS       DAOTBO010000045        19751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2258_length_19751_cov_5.998172, whole genome shotgun sequence.
ACCESSION   DAOTBO010000045 DAOTBO010000000
VERSION     DAOTBO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 19751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19751
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_19751_cov_5.998172"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..543)
                     /gene="def"
                     /locus_tag="PLE88_06815"
     CDS             complement(19..543)
                     /gene="def"
                     /locus_tag="PLE88_06815"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOM76211.1"
                     /translation="MSDYSKCTITRYPTPVLLEKAQPITDIDDGIRKLAERMIDIMVE
                     NKGIGLAGPQAGVNLRIFVISLDGSKENAKVYINPTIKPEGPVVVNEEGCLSLPGLWG
                     KIKRYSRCTVTATDLNGQTFTETGEGLLARALQHEYDHLEGRMIKDRLSQPAKLRARH
                     RLKQLEEEYKNQAK"
     gene            complement(568..1320)
                     /gene="lptB"
                     /locus_tag="PLE88_06820"
     CDS             complement(568..1320)
                     /gene="lptB"
                     /locus_tag="PLE88_06820"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOM76212.1"
                     /translation="MTLLETQGLVKKYSGRTVVNEVSITIGQRSIVGLLGRNGAGKTT
                     TFRMCIGMIVPDGGRVFFEGHDVTTLPMYKRARLGMGYLSQEPSVFQRLTVRENLLAI
                     LETMAVSSSERKRRADDLIERFGLGEVVSSQARFLSGGERRKLEIARAMVTNPSLILL
                     DEPFSGVDPIAVEELQAEIRRLVACGVSILITDHNVERTLEVSDKAYIIDHGKVIAEG
                     TPKAIIQNELVKKSYLGSTFKGDEFDEPPATS"
     gene            complement(1415..3562)
                     /locus_tag="PLE88_06825"
     CDS             complement(1415..3562)
                     /locus_tag="PLE88_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOM76213.1"
                     /translation="MDNFAEIALLLVLAAALGAVGLKLRQPLIIMFLAVGILAGPSGL
                     GIIESYSQIELLAHIGIALLLFIVGLRLDINLIRTIGPVALATGLGQIIFTSAIGFVI
                     ALLMGMPVISAVYVAVALTFSSTIIIVKLLSDKKEIDSLHGQIAVGFLIVQDIAAIVA
                     LIILTTFRPQAAAEHSPLISTFLIAVKGLLFFGCIGLLMRFILPRLRRLAQSPETLLL
                     FAVAWAVFLAAVSEWLGFSKEVGAFMAGVSLASTEYRDSISSRLSSLRDFLLLFFFID
                     LGARLEWSTVGAGLTNAVIFSLFVLIGNPLIVLIIMGLMGYRRRTSFLAGLTVAQISE
                     FSLIVAAMGVSLGHIPEETMGLITLVGVTTIFVSTYMILYSGPLYRLLAGPLKLFERK
                     NPYRETVEGGAAKSDTVDVILFGLGNYGSGLAEHLLERRKSFIGVDFDPTVLDKWRRQ
                     GIPVLYGDLGDPDLHEQLPLDRARWVVSAIRSRELNLTLLGLLKHRGYTGKIALTAVN
                     EKEAKLYEKQGAQVVFRPFHDAAEQAVDALTQAMELLTSNVNWPAAFSEIRLRPGSAY
                     AGQTIRSIPLRAQTGASILAVSRAGTVHYDPGADFQVFPGDRLVIVGTPEQLARAQEL
                     LNHFPQPPAEEEIRFVMDQLPITENSPQAGQTLADLQFRVKYNVTVIGICRGEQKITS
                     PGPNEKLSAGDRLIIIGTAQAVSRLKNSAPASA"
     gene            3811..7194
                     /locus_tag="PLE88_06830"
     CDS             3811..7194
                     /locus_tag="PLE88_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOM76214.1"
                     /translation="MFSKTAALCFIIGLSVSILPSQAATSVKIVSLDAYDVVYDSFSQ
                     RIYASVPSAAAAYGNNLAVIDPVSGVIEDSVFVGSDPQKLAVSGDGEYLYIGLNGAGS
                     VSRFHIPSKTVETFSLGTGSDATTYAEDIAVQPGNSKVIAVSRYRKGVSPRNYGVAVY
                     DNGIKRPQQISGANVIEFSDSPGVLYGYNNETTEFAVRTLIVNDYGVSTAAAKSGLIS
                     GFGVDMEYADGFLYSTNGGVVNTQLMQLAGQFGVSGKLEPDPMVRRVFFADGATLKAF
                     NTTTFLLEGQETVSGVSGSPRNLIRWGSKGLAFSTSGKQVVIVETDLVPLPPAVIRLT
                     IDGPDYLTSYKGQYKLIAEFDDGTVADVTRKSRWTTVPDTYTHIDNTGRLYVLGTDQA
                     GTANISAEYVFAGTQYTAVKTISYEGIIPAAGNLVRLQIDGPSQVLQQSTVQMTGTAF
                     FDDGASYDVTGKAVWSLGSTDFARIDQTGLLTLGAISRPRDLIIYASFGYKDAEAQAA
                     KTVIVLEDASQVSSCDWPTYQGDPQHTGFAAVSLNPQDFSLRWAKTISAGSAMKPVSA
                     AGGRVFIAVNSALLCLDARDGEILWSQPLNSPHHQPTYAYRYVYVQTDQHSPGYIRAF
                     EADTGAQIFASTFSQQWDTWWAPVVYDGEIYIAGGYYGGAGAYNAFTGEKKWNVSLPQ
                     YDAWTPAVKGELMFAYVGGTLYALERKYGTTSYTINDPSFSWQGWSMNLAPVLSDAEN
                     VLVIYNGRLKCFDLNAKNIRWEIAGGFSGQPSVAEGMVYAVNSGMLEARDEMDGSLIW
                     SWSAPEGSLKSPLIATRTHVIACTNANTYAVELLSRQSEWSYPASGYLALGNDTLYIA
                     GSNGVLTAIAVPEYIPAEPIKLEIEGPIDIFESSANAYQAIVTYSDGRVRDRTALCSW
                     SVTEKPWCSLDQGLLHVGELLYPQETVALSAVYRQDNGSVADTRLITIHANAAEEGII
                     ARNLEKAMQSKQKILYELQQAMMYEAASGSIAEDLKQKSSKSNSGISRPLLNKAVNQI
                     EHTLNFETVSRKHLEASIVNLAAALEIFASDSPSLGIASGMVVPQMSAAAEAVGCPYA
                     LTGDLDSNCSVNMTDLMILAENWLVDCSLQPANPACQQK"
     gene            7379..8896
                     /locus_tag="PLE88_06835"
     CDS             7379..8896
                     /locus_tag="PLE88_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOM76215.1"
                     /translation="MLQYDKCVRAVFAFCCIGLSGFAVSGESQTDYLAIMLSGQKIGY
                     AVHTRTEADDKVTTAERLQMTLGRGGQGMKVTMEETHIETADGKPLGFEMVMNTSGME
                     QKTTAAVKDGKAVVTRYVMGQPQEMTIDWPADALLNEGLRLYQLKRGLKAGDAYEIKI
                     FRPDMLMALTAQISIGEKTMIDLFGRMLELTQVESTLQVGGQQITVTSYVDESTRALK
                     SRVPMMGMVLELIACDKSVALREDDIVDFLDKMTIASPIKLMNLNKIGSITYILKPTE
                     DTKKLDIPTSLSQLVETMPDGIAVTVRPKVPAGPIAFPYKGDNPEALEALKPTDYLQS
                     DNEVIINLAKNAVSGTDDAAVAARRIEAFVDGFITKKDLSVGYASAAEVAKSRQGDCT
                     EHAVLAAAMCRAAGIPARVVCGVVYADSFLSKQNIFGGHMWTEVWLGDGWYGLDATRS
                     GEGGFGAGHIALAYGSGDPIDFFGLVNTLGCFKITHISVTAKSDAAQPEPLSVRN"
     gene            8919..9113
                     /locus_tag="PLE88_06840"
     CDS             8919..9113
                     /locus_tag="PLE88_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76216.1"
                     /translation="MNQNACSNHENHLCWLYEAGLPQNEPDRYALLVRNPKYVCKNCG
                     RVAASKENLCAAVLLGTWEE"
     gene            complement(9156..13442)
                     /locus_tag="PLE88_06845"
     CDS             complement(9156..13442)
                     /locus_tag="PLE88_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOM76217.1"
                     /translation="MAASKAIRSGFIFTVLLISLSFGLDLEPEQTTAVLPQHNHLDVF
                     VMSYCPFAQAAEAGLLNYLIAMPAGQSFEIEIHYIFYKTIQDGTIVYTCLHGEKEVQE
                     NLVQMMIRDLAPEYFHLYLLARIGSRAPWQELAAGVGLTQAEIEHIQTAIELLRDELI
                     EREYAVTTQTYKIYDGSPAYVWNGERIKNLTDIGVFKDLQFEFLGVRCSPDGVYDPNP
                     KTEQERQEMHQRTAQQTRLHQSEQPPAAELYRDVSETVKNELLLKIKEPQPHRFRGLE
                     IGDLLVFHSQRYIGQAEVEKDQIVYHFDRQTSEHKNTIRHWRSDLPDTLPPIISIHEA
                     MAKVQHPDGARLVYISPDSDIFPLDPIPSNPCWVVRNLGEGGLELIVIDAVTGQELGF
                     GIAPPATAYSMTGPTDYNPCSGNWNEWADSARDWFNTMGYSTDRITWPSRANIQSHIQ
                     SSSIALFYELAHGGSTYFSSGCQSNGNFYENTYASDIETWITAYSKMRFTFVGSCEGL
                     CNTGDNTFAYEFSKGSTNDTVAVGYCHMDATYCETCWSNSIAWQNKLFERMNAGDTIR
                     QAFNAANAAYPACVGNASGDCTRFYGDTALKAKPLLKREPDCGDTVTGDVLLSRNLYC
                     AGNGLIVGDNDLTIDCQGHLIDGNGTGIGIVNNGYDNVTIKNCTIREFGTGIYLYGAA
                     DYNKLEYNTVYSNTGHGISLNNSWWTDINQNNVHSNSYNGLYLYNSKYAEVIDNDFTS
                     NGWHGLQIMPQEGISGSGGNNTFEINYLYGNSWSGAGINTSSNTFTGDRANSNKAYGF
                     HITDSTGNTFTDCDAMSNVDGFYIANAANTTITSTIPNNTDIWYNIDYGVYIANSTNT
                     VVEKSHLYNTAETEDSETDTAISISNSSGTVIRSNTLGFNERGVYVNNSDGTAIQNNV
                     FGNTETVDILLLNSDTCSITGNAMDNNTGSGIQLHSSHNNSIQNNTINNSTYYGFYLN
                     GSTLNTYIGNTVTRSGSYGVYAIGGSNSNDFTGNFFSMNNRGIFISGSIGNEFTGNTV
                     CSSVYQDFYIASEAIGTTTGNNNICDSVYNYADIGQVYGCDTMCSGCRRLEDELVITA
                     NTTVCPETYNITDAGTLGIVRFGAGGITLDGNGAVLIGPSADTGYGIISNGYDNVTIK
                     NLTVKNYFILIDGRDGENIQVLNNRLDNAAAGIRFWGVNYGKMLNNCITGVNQYGAVV
                     TSSSTACKLIYNTIADSPSIGAVFCGNGSSALLANSILWNNTGWQIMMNTASGPSQLD
                     VSYCDIEGGTSDVSIGSGCTLNWGWGIIAVNPLFAGSGDYHLQSQAGRWNPLTSSWTA
                     DTFTSRAIDAGNPGMSLSNEPVHRPNLRVNLGAYGNTAQASKTPLGWSLLTDINNNGT
                     VNLADFVCFSSQWLRNDTYDLPSDFTRDSVVSLSDLLYMTKEWLLQTAWH"
     gene            13897..>19751
                     /locus_tag="PLE88_06850"
     CDS             13897..>19751
                     /locus_tag="PLE88_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOM76218.1"
                     /translation="MRKSFLDIAYLVLILAIIIGSALGDCPSGDITGDCIVDIDDLFV
                     AAENWLSSQMPLHSGLIAHWGFDEQAGETAADSAGGHTGQLYGEPQWVRDEEFQREAL
                     IFDGVDDYIEVPDYKGIAGTASRTCTAWIKTSKTNSHIINWGLQSQEGGMWAVLVGDT
                     GALRVQVSNGYIYGVTNIADGEWHHIAVVLQGDGSPDISEAMLFVDGLSETSIGGVLA
                     KPVNTVTSENVKIGVNILKTAYLQGRLDEVQIYNQALREDEILRLYQTGNALTHNPDF
                     TGDGRVDTEDFIPLSDQWLRAEPAVIISEFLASNDAANPPDISQGQIRDGNGESSDWI
                     ELYNQTDFPIALGGWGLTDNAGNPLKWQFPSGTVLSGHSYLIVFASGKEQSDYPSNYP
                     YVDPQGFLHTNFKLSADGEYLALTRPDGTVEHAYDSVLNTVSGHYGFPPQEKNISYGM
                     LHNEEYFFALPSPGQSNRKSFLGFVEAPEFSHERGFYETSFWLTLNTSTPDAVIRYTT
                     NGTDPTLSNGTTYTGPIAVSVSTSPVGRCIRAAAFKPGYQASPIKTRTYLMKATEYMK
                     GLPAVCLSGSPTQVFYNPNGIMAIVGGTWVNGVWQQVNSTDYNNVLGHGMDYERPVSM
                     EYIRRTDGTEFQEDCGIRVHGSAWMRPRYTIPSVSDAWYGTSKYSLRLYFRSRYGDNT
                     FAHPILEQFPEVDEMDTFVLRAGHNDQTNPFVRDEMIRRLQYQMGNQTARGTFVNLYI
                     NGVYKGYYNLCERINEDYCQKWFNSDKPWDVVGWVATGYSFYLEARDGDTKAFEDFIN
                     YAKNNNLANPLYYAQVEQQMDMPAFVDYIIVQTWGGNWDWPQNNWTAAAERSAARKWR
                     FFVWDAEGCMDGDTSRNRFSNLNTDNSPLSVLYRALSVNQDFKILFADRLQKHFLDAG
                     GAMTQANLKALFWQLAGEVKGVIPDISTYIPNTYIPVRESIYFTQCRNQDLFKFAAPR
                     VFLYGREIQKDYALPGAQITMQNADGVSGDIYYTLDGKDPRLTLVERANTATTTLVAE
                     NASKRVLVPTKDIGTTWTQVNYDDSLWNDGLPADNSKTGGVGYERNPGEATSTLPYVS
                     YNVESKMYNNYTSAYVRIPFIVDAGQMQSWNYLSLKMRYDDGFVAYINGTEVCRVGIS
                     GPPAWNSTAPSHENSSLETFIISNYLYLLQPGLNILAIHGANYSKNSSDFLVSAILEA
                     GYDSANGIAPSAQKYTGPISLSKSVHIKARTLSGTQWGALREAFVSVGTISDNLRISE
                     FLYNPDGDPNEEFIELTNIGTTPLNLNQVSFTKGIQYTFGDTVINPGQYLLLAADKTV
                     FENRYGTGLPVVGEYAGKLDNAGETIQISDILGNPIQTVNYKDSWYEIADSDGFSLTA
                     VDPAYEIPISSDDLAAHWKFDETTGTTVYDMAGLHHGTIYNMQDTARVYGAKENALLF
                     DGTNDYIAVGGYKGISGSASRTCTAWIRTGTVMSQIISWGELQTGRKWAVRVNDDGTL
                     RAEVQEGYIYGTTPVADNQWHHVAVVLIDDGSPDISEAILYVDGKAETTFGGIQARAV
                     NTAAVSDVLIGVNLNGTVFFQGLMDDLRIYSRALTADEIAAMIAQVNWDSKDLWRPSA
                     VRGGTPGRDETPTERVPLPGAIVINELLAHSHAGLPDWIELYNTTNQAIDISGWFLSD
                     SFSSQTNRKKYRIPDGTILTPANPYYVLEETAFNNISDAGCRIPFALSEGGDTLYLQS
                     ANGEELTGYFTKEEFGATASGVTLGRFQKSTGTWNFVPMSVPTKGADNAYPLVGPVII
                     TEIMYNPGPNPIDQDYEFLELMNISSQPVRTASYMSTYTSPDIHIEEWIPWRFTNGIE
                     FEFPLDLQLEPGQRILLVKKLSSFNSRYTGVPAGTVIFQWTSGSLANEGETLQLAMAG
                     DQEYQKERYYIREDRVNYDDQSPWPTQANGTGLSLTHLRPMETGNNYTNDPV"
BASE COUNT         4927 a         4670 c         5279 g         4875 t
ORIGIN      
        1 atgtatccaa tcgttttgtt attttgcctg atttttatac tcttcttcaa gctgcttaag
       61 acggtgtcgg gctcggagtt tggctggctg gcttagtcga tctttaatca tccgcccttc
      121 aagatggtca tattcatgct gcaaagccct tgccagcaga ccttcgccag tttcggtgaa
      181 tgtctgccca ttcaggtctg tggcggtaac tgtgcagcgg ctgtaccgtt ttattttacc
      241 ccagaggccg ggcagagaca gacagccctc ttcattcaca acaaccggtc cctctggctt
      301 gatagtcgga ttgatgtaaa ccttggcgtt ttcctttgag ccgtccagag agatgacaaa
      361 gatgcgcaag tttacaccgg cctgcgggcc ggccagaccg atgcccttgt tttcaaccat
      421 gatgtcaatc atccgctcgg ccagcttgcg gataccgtca tcgatatccg taatcggctg
      481 cgccttttcc agcagtaccg gcgtcggata gcgtgttatt gtgcatttgg aataatcaga
      541 catggttcag tcctattgac aggtttgtta gcttgttgcc ggcggttcgt cgaactcgtc
      601 ccctttgaag gtgctgccca agtagctctt cttgaccaac tcgttctgga tgatggcctt
      661 cggtgtgcct tcggcgatga ctttgccgtg gtcgataata taggctttat cggacacttc
      721 caatgttctt tcgacattgt ggtcggttat caggatgctg acgccgcacg ctaccagccg
      781 gcggatttcg gcctgcagtt cttcgacagc aatcgggtcc acgccggaaa acggctcatc
      841 cagcagtatc agagaaggat tggtaaccat tgcccgtgca atctcaagct tccgccgctc
      901 cccgccggac aaaaaccgtg cctgactgct gacgacttca cccagcccga accgctcgat
      961 gagatcgtca gcgcggcgct tgcgttcgga ggagctgaca gccatcgttt ccaaaatagc
     1021 cagcaggttt tcgcgcacag tcagcctctg aaagacgctg ggttcctgcg acagatagcc
     1081 catacccagc cgggcgcgtt tgtacatcgg cagggtggtg acatcgtggc cttcaaaaaa
     1141 aactctgccg ccgtcgggca cgatcatacc aatgcacatc cggaaggtgg tggtttttcc
     1201 ggcaccgttg cgtcccagca agcctacaat cgagcgctgg ccaatagtaa tgctgacctc
     1261 attgactacc gtccggccgg aatatttttt taccagaccc tgcgtttcca gaagcgtcat
     1321 atcaaccaat ctgtatctcc taaaacagga attttctgaa acaacagtat tataccagca
     1381 ttttgtactg tgtaaaatcc ctttttggag ctggttatgc actggcaggg gcactgtttt
     1441 taagccggct gacggcttga gcagtgccga taataatcaa tcggtcgcca gcagacagtt
     1501 tttcattggg gccgggagaa gtgattttct gttcgccgcg gcagatgccg attacggtta
     1561 cattgtattt tacccgaaac tgtaaatctg caagagtttg gccggcttgg ggggagtttt
     1621 cggtgatagg cagctggtcc atgacaaagc gtatctcttc ttccgccggc ggctggggaa
     1681 aatggtttaa aagttcttga gcgcgtgcga gttgctcggg ggttcctaca ataaccagcc
     1741 ggtcgccggg gaagacctga aaatcggctc cgggatcata atgtacagta ccggctctgc
     1801 tgacggccag aatagaagcc ccggtttgtg cccgcagcgg aatagagcga attgtctggc
     1861 cggcataagc cgaacccgga cgcaagcgga tttcactaaa tgcagccggc cagttgacat
     1921 tgctggtaag cagttccatt gcctgagtaa gggcatctac agcctgctcg gcggcatcat
     1981 ggaaggggcg gaatacaacc tgtgccccct gcttttcgta aagctttgct tctttttcgt
     2041 tgacagctgt cagagcgatt ttgcctgtat aaccgcgatg cttgagcagt cccagcaggg
     2101 tgagatttag ctcgcgcgag cggattgcac tgactaccca gcgtgctcta tccagcggca
     2161 gctgctcgtg caaatccgga tcgcctaagt cgccataaag gactgggata ccctgccgac
     2221 gccatttatc cagaacagta gggtcgaaat cgacaccgat aaagcttttg cggcgttcaa
     2281 ggagatgctc agccagtccg ctgccgtaat tgcccagccc aaagagaata acatctaccg
     2341 tatcggattt agcagcgccg ccctctacgg tttcgcggta gggatttttc cgctcaaaca
     2401 gcttcagagg accggcaagc agacggtaga gaggaccgga atacagaatc atatatgttg
     2461 aaacaaatat cgtagtaact ccgactagcg taatcagccc cattgtttct tccgggatgt
     2521 ggccgaggga aacgcccatt gcggctacaa taagcgaaaa ttcgctgatt tgggcgacgg
     2581 tcaggccggc taaaaagctg gtgcggcggc ggtagcccat tagtcccata ataatcagga
     2641 caatcagtgg attgccaatc agcacaaaca gggaaaagat aacggcgttt gtcagaccgg
     2701 cacctacggt agaccactcc agccgggcac cgaggtcgat gaaaaagaac agcagcagaa
     2761 aatcccgcaa actgctcagc cgggagctga tagaatcccg gtattcggta gaagccagcg
     2821 aaaccccggc cataaaggca ccaacctctt tgctgaagcc cagccattcg cttacggcag
     2881 ccagaaagac tgcccacgcc accgcaaaca gcagcagtgt ttcgggcgac tgggccagcc
     2941 ggcgcagacg cgggaggatg aagcgcatca gcagaccgat acagccgaaa aacagcaggc
     3001 ccttgacggc tatcaggaaa gtggaaatca gaggggaatg ctctgctgcg gcctgcggac
     3061 ggaaggtcgt caggataatc agtgcaacaa tggcggcgat gtcctgtaca atcagaaagc
     3121 ccacggcaat ctggccgtgc agcgagtcaa tttccttttt atccgacagc aatttgacga
     3181 tgataatcgt gctggaaaag gtcagggcaa cagcgacata aacggcactg ataaccggca
     3241 tacccatcag cagggcaata acaaagccga tagcgctggt aaagataatc tgccccagac
     3301 cagttgccaa ggcgacaggt cctatggtcc ggataagatt gatatccagc cgaagcccga
     3361 caataaacag cagcagtgca atgccaatat gggccaatag ctcgatttgg gaatagcttt
     3421 cgatgatgcc taagccggaa ggcccggcca gaatgccgac ggccagaaac ataataatca
     3481 gcggctgacg gagcttgagc cccactgctc ccagtgccgc ggccagtacc aaaagaagcg
     3541 ctatttcggc aaaattatcc atttgcagca tccccaagac aagtttttga ctgcactttt
     3601 atgccacggc ccctaatacg aggaagaaaa gagcacaaac ggacacagta agaccagtct
     3661 aaaccactct tcttatggag aaagtgtatc tgtttttgat tcgaattaca aaattttttt
     3721 ggtagatgga tagatttttg ataaaactgg gatatctttt tgttgttcga ttttaacaca
     3781 tatgacaaca ttattacagg agggtatttt atgttttcaa agactgctgc tttatgtttc
     3841 ataatcggat tgtctgtgtc catcctgccg tctcaagcgg ccacttccgt caagattgta
     3901 agtttggatg cctatgatgt tgtttatgac agtttcagtc agcgaatata tgcttctgtg
     3961 ccttctgccg cagccgctta tggcaacaat ctggctgtta tagatcctgt ttcaggagtg
     4021 attgaagatt cggtttttgt cggcagcgac cctcaaaaac ttgctgtgtc aggcgatggg
     4081 gaatatttat acattggcct gaatggcgca ggcagtgtaa gccgatttca tattccttct
     4141 aaaacggttg aaacattttc tctgggaacc ggctctgatg cgacaacata tgctgaagac
     4201 attgctgtac agccgggcaa ctcgaaagtc atcgctgtgt cccggtatcg caagggtgtc
     4261 agcccgcgaa actatggcgt tgctgtctat gataacggca tcaaacggcc tcagcagatt
     4321 tcaggagcta atgtgataga gttttcggat tcccccggtg tgctttacgg atacaacaat
     4381 gaaacaacgg aatttgctgt tcgtactctg attgttaatg attacggtgt ttcgacagcc
     4441 gccgctaaga gcgggcttat cagcggcttc ggcgttgata tggaatatgc agacggcttc
     4501 ctgtattcga ccaacggagg tgttgtcaat acgcagctta tgcagctggc aggccaattc
     4561 ggggtcagcg gaaaactcga accggatcct atggtgcgcc gggtgttttt tgcggacgga
     4621 gcaacgctga aagcgtttaa tacgacgaca tttctgctgg aggggcaaga aactgtttcg
     4681 ggcgtttccg gctcaccccg aaatctgatt cgctggggca gcaaaggatt ggcgttcagt
     4741 acaagcggaa aacaggttgt cattgtggag accgatcttg tgccgcttcc gccggctgtt
     4801 atccgcttga ccatagatgg accggattat ctgacaagtt ataaaggcca atataaactg
     4861 attgccgaat tcgatgacgg cacggttgcg gatgttacgc ggaaatcccg ctggacgact
     4921 gtgccggata cttataccca tatagacaat acaggaagat tgtatgtctt gggcacggat
     4981 caagcaggaa cggcgaacat ttcagcagaa tatgtgttcg ccggcacaca atataccgct
     5041 gtcaagacca tttcctatga ggggattatt cccgctgcgg gcaatctggt ccggctgcag
     5101 atagacggac cgtctcaggt acttcagcaa agcacggtac agatgacagg aacagccttt
     5161 tttgatgatg gtgcgagtta cgatgtgacc ggcaaagccg tttggagtct gggcagcacc
     5221 gactttgcaa ggattgatca gaccggtctg ctgacgttgg gtgctatcag ccgcccgcgc
     5281 gatctcatca tctatgcatc gtttggctac aaagatgcag aagcccaagc ggctaaaacg
     5341 gtaattgttt tggaagatgc ttcgcaggtc agcagttgcg actggccaac ctatcaaggc
     5401 gatccgcagc atacgggttt tgctgccgtt tcgctgaatc cgcaagattt ctccctgcgc
     5461 tgggcgaaaa ccatcagtgc cggcagtgct atgaagcctg tgtccgccgc cggcgggcga
     5521 gtttttattg cagtcaattc ggccttgctg tgtctggatg ctcgagacgg cgaaatactc
     5581 tggtcgcagc ccttgaattc gccgcaccat cagccgacct atgcttatag atatgtttat
     5641 gtccagaccg atcagcacag tccggggtat attcgtgcct tcgaagcaga caccggcgcg
     5701 cagatatttg caagcacatt cagtcagcag tgggatacct ggtgggcgcc ggttgtgtac
     5761 gatggggaaa tctatattgc cggcggctac tatgggggtg ccggggccta caacgcattt
     5821 accggtgaga aaaagtggaa tgtgagtttg ccgcaatatg atgcgtggac gccagcagta
     5881 aagggcgagc ttatgtttgc ctatgtaggg ggaacgcttt acgcattgga acgtaaatac
     5941 ggaacgacgt cttatacgat aaatgaccca tccttcagct ggcaggggtg gagcatgaat
     6001 ctggcaccgg tactcagcga tgctgagaat gttctggtga tttacaatgg gcggttgaaa
     6061 tgctttgatc tgaatgctaa gaatatccga tgggaaattg ccggcggttt cagcgggcag
     6121 ccgtctgtgg cagagggcat ggtttacgcc gtaaacagcg gtatgcttga agccagagat
     6181 gaaatggacg gcagcttaat ctggagctgg tcggcgccgg agggcagcct gaaaagcccg
     6241 ctgattgcca cccgcacaca tgttatagcc tgcacgaatg ccaataccta tgctgttgaa
     6301 ctgctcagcc ggcaatcgga atggtcttat ccggccagcg gctatctggc attgggcaac
     6361 gatactctgt atattgccgg aagcaatggt gttttaacgg cgattgcagt gccggaatac
     6421 attccggctg aacctattaa gctggaaatt gaaggaccga ttgatatttt tgaaagctct
     6481 gcaaatgcct atcaggccat tgttacctac agtgacggcc gtgtgagaga tcgaacagct
     6541 ttgtgcagct ggtctgtaac tgaaaagccg tggtgttcgt tggatcaggg actgctgcat
     6601 gttggtgaac tgctatatcc gcaggagaca gttgcgctga gcgcagttta caggcaggac
     6661 aatggatcgg ttgccgatac ccgcctgatt accattcatg ccaacgctgc agaagaaggc
     6721 attatcgcac gcaatcttga aaaagcgatg cagtcgaagc agaagattct ctatgaattg
     6781 cagcaggcga tgatgtacga ggcagcatca ggcagcattg ctgaggacct caagcagaaa
     6841 tcgtctaaat cgaactccgg catcagccgg cctctattaa acaaggcagt aaatcagata
     6901 gagcataccc tgaattttga aacggtatcc cgtaagcatc tggaggccag tatcgtcaat
     6961 ctggcggcag ctttggagat atttgcttca gacagtccgt ctcttggcat tgcatcaggt
     7021 atggtagttc ctcaaatgtc tgcggctgcc gaagctgttg gctgtccgta tgctctaacc
     7081 ggggacctgg attcgaattg cagtgtcaac atgaccgatt taatgattct ggccgaaaac
     7141 tggctggttg actgcagttt gcagccggcc aatccggcgt gtcagcagaa ataaaaatgg
     7201 agcaaaacaa aaagccgctg cctgttctcc ctaagcagcg gctttttttg ttttgtttga
     7261 gttcttggcc ggttcggata caatagatgc agaaatagag aatttcccaa acggctttcg
     7321 gcacgatttg ggcttagaac ataactcatt ttgggaataa agagtgaaag gataggcgat
     7381 gcttcaatac gataagtgtg ttagggctgt atttgctttt tgttgtattg gactgtcggg
     7441 atttgcggtt tctggagaaa gccaaaccga ttatctggcg attatgctgt ccggccagaa
     7501 gattggttat gctgtgcata cccggaccga ggccgatgac aaagtaacca ctgccgaacg
     7561 cctgcaaatg actcttggac ggggtggtca aggcatgaaa gtaacaatgg aggagaccca
     7621 tattgaaacc gctgacggca agccgttggg atttgagatg gtgatgaaca ccagcggcat
     7681 ggagcagaaa actaccgccg cagtcaaaga cggcaaggcc gtcgttaccc gttatgtgat
     7741 gggtcagccg caggaaatga cgatagactg gccggccgat gcgctgctga atgagggcct
     7801 gcgtctgtat cagctcaagc gagggctgaa agccggagat gcatatgaga taaaaatatt
     7861 ccgccccgat atgctgatgg ccctgacagc acagatttcc attggcgaaa agacaatgat
     7921 tgatttgttc ggccgcatgc tggaactgac acaggtggaa tcaacactgc aggtgggggg
     7981 gcagcagatt actgtcacca gctatgtgga tgagtcgacc cgtgcgctga agagccgggt
     8041 gccgatgatg ggaatggtgc tggaattgat tgcctgcgac aaaagcgtgg ctctgcggga
     8101 agatgatatc gttgattttc tcgacaaaat gaccattgcc agtccgatta agctgatgaa
     8161 tcttaacaag attggttcga ttacctatat tttaaaaccg acagaggata ctaagaaact
     8221 ggatatcccc acttcattgt cccagctcgt agaaacaatg cccgatggca tcgcagtaac
     8281 tgtaagacct aaggtgcccg ccggtccgat tgcttttccg tacaaaggcg ataatcctga
     8341 agcccttgaa gcgctcaagc cgaccgatta cctgcagtcc gataatgaag tgattatcaa
     8401 tctggcgaaa aatgccgtca gcggtacaga cgatgcagcg gttgcagccc gccggattga
     8461 agcctttgtg gatggattta ttaccaaaaa agatttgtcg gtcggctatg cctctgcggc
     8521 tgaagtagcc aagagccggc aaggcgactg caccgaacat gctgtattgg cggcagcgat
     8581 gtgccgggcc gcgggcattc cggcacgggt tgtctgcggg gtcgtatatg cggactcttt
     8641 tttatcaaag cagaacattt tcggcggtca tatgtggacg gaagtctggc ttggcgacgg
     8701 ctggtacgga ctggatgcaa ctcgttccgg cgaaggcggc tttggggcgg ggcatattgc
     8761 gctggcctac ggcagcggcg acccgataga cttctttggg ctggtaaata cgctgggttg
     8821 ctttaaaatt acacatattt cggtaaccgc caaatcagat gctgcccagc cggagccttt
     8881 gtccgttcga aactaatact aaataaggtc ttgatgcaat gaatcaaaac gcctgttcca
     8941 atcatgaaaa tcacttatgc tggctgtatg aagccggcct gccgcagaat gaaccggacc
     9001 gctatgcact tttagtgcgc aatccaaaat atgtctgcaa aaactgcgga cgggtagcgg
     9061 ccagcaaaga aaacctctgc gcagccgttt tactgggcac gtgggaagaa taatctgccg
     9121 cacgctggat gtgccgctgc ggcggattaa agcagttaat gccacgcagt ctgcaagagc
     9181 cattccttgg tcatataaag caggtctgac aggctgacaa cggagtcccg cgtgaaatca
     9241 gacggcaggt cataggtgtc attgcgcagc cattggctgg aaaagcagac aaaatctgca
     9301 agattgacgg tgccgttgtt gttaatatcc gtcagcagac tccagcccag aggagttttg
     9361 ctggcctgtg cggtgttgcc ataggccccc aaattgacgc gcagattggg ccgatgaaca
     9421 ggttcattag acagactcat tcccggattg cccgcatcaa tagccctgct ggtaaaggta
     9481 tcggcagtcc agctggacgt taatggattc catcggccgg cctgagattg caggtgataa
     9541 tcgccgctgc cggcaaaaag cggattgaca gcgatgattc cccagcccca gttgagtgtg
     9601 cagccggaac cgatgctgac gtcgctggtg ccgccttcga tatcacagta ggagacatcc
     9661 agctgggaag gaccagatgc ggtattcatc ataatctgcc agcctgtgtt gttccacaag
     9721 atgctgttgg ccagcaaggc ggagctgccg ttaccgcaga aaaccgcgcc gatagagggg
     9781 ctgtcggcga tggtgttgta aatcagtttg caggcggtac tgctggaggt aacaacggct
     9841 ccatactgat tgacgccggt aatgcagttg ttgagcatct tcccgtagtt gacaccccag
     9901 aagcggatgc ccgcagcagc attgtcaagg cggttgttga gcacctgaat attctctccg
     9961 tcgcggccgt ctatcagaat aaagtaattt ttaaccgtta gattcttgat ggttacattg
    10021 tcgtatccgt tgctgatgat gccgtatccg gtgtcggccg atgggccgat taaaacggcg
    10081 ccgtttccgt ccagtgtgat tccgccggcc ccgaagcgca caatccccag cgtgcccgca
    10141 tcggtgatat tgtaagtttc cgggcaaaca gtcgtattgg ctgtaatgac cagctcatct
    10201 tccagccgcc ggcagccgct gcacattgta tcgcagccgt aaacctggcc aatgtcggca
    10261 tagttgtata cggaatcgca gatattgttg ttgccagttg tggtgccgat ggcctcagag
    10321 gcgatataaa aatcctgata aacggatgaa cagactgtat tgcccgtaaa ttcattgcct
    10381 atgctgccgg aaatgaatat gccgcggttg ttcatactaa agaaattgcc ggtaaaatcg
    10441 ttggaattgg atccgccgat agcatagaca ccgtagcttc ctgaacgggt taccgtgttg
    10501 ccgatatatg tgttcagggt cgagccgttc agataaaagc cgtagtaggt cgaattgttg
    10561 atggtgttgt tctgaatgct gttattgtgg gaggaatgca gctgaatgcc ggagccggtg
    10621 ttattgtcca ttgcattgcc ggtaatgctg caggtatccg aattaagcag caggatatcc
    10681 acagtttcag tattgccgaa cacattattc tgaatggcgg ttccgtcgga gttgttgaca
    10741 tagacacccc gctcattaaa gcctaatgta ttgctgcgga taacagttcc cgatgagttg
    10801 gaaatagaaa tagcagtatc cgtttcgctg tcttctgttt ccgctgtatt atacagatgg
    10861 cttttttcaa cgactgtatt ggtagaattt gcaatataga cgccgtaatc aatattgtac
    10921 cagatatccg tgttgttagg gatggtggag gtaatggtgg tattggccgc gttagcaatg
    10981 taaaaaccgt cgacgttgga catagcatcg caatcggtaa aggtgttgcc ggtgctgtcg
    11041 gtgatatgga atccgtaggc cttgttggag tttgctctgt cgccggtgaa ggtgttgctg
    11101 ctggtattga tgcccgcacc gctccagctg ttgccgtata ggtaattgat ttcaaaggta
    11161 ttattgccgc ccgaaccgct gatgccctct tggggcataa tctgcaagcc atgccagccg
    11221 ttggatgtaa aatcgttgtc aatgacctcg gcatattttg agttgtagag atataagccg
    11281 ttgtagctgt tggaatggac attgttttga ttgatatcgg tccaccagga attatttaag
    11341 gaaataccgt ggccggtatt ggaataaaca gtgttgtatt caagcttgtt gtagtcggct
    11401 gctccgtaaa gatagatgcc tgtaccgaac tcgcgtatgg tgcagttttt gatggtgaca
    11461 ttgtcataac cgttgttcac tatcccgata ccggttccgt tgccgtcgat aagatgtccc
    11521 tgacagtcaa tagtcagatc gttgtcaccg acaatcaatc cattgccggc acagtagaga
    11581 ttgcggctta gcaagacgtc gccggttaca gtatcaccgc agtccggttc gcgtttcagc
    11641 aggggcttgg ctttcagtgc agtatcgcca tagaagcggg tgcagtcgcc gctggcattg
    11701 cctacgcagg ccggataggc agcattggca gcattgaaag cctgacgaat ggtatcaccg
    11761 gcgttcatcc tttcaaacag cttgttttgc caagcaatgg aattgctcca gcaggtctcg
    11821 cagtaagtgg catccatatg gcagtatccg acagccacag tatcattggt gctgcctttg
    11881 gaaaactcat aggcaaaggt gttgtcgccg gtattgcaca gaccttcgca gcttcccaca
    11941 aaggtgaacc gcattttgct gtaggcggta atccaggttt cgatgtcgct ggcataagtg
    12001 ttttcataaa aattgccgtt gctctgacag ccgctggaaa aataagtgct gccgccgtgg
    12061 gccagttcgt aaaacagtgc aatggaggag ctttggatat ggctttgaat gtttgcccgt
    12121 gagggccacg tgatgcggtc ggttgaatag cccatggtgt tgaaccagtc gcgggcgctg
    12181 tcagcccatt cattccagtt tccgctgcag ggattgtaat cggtgggtcc ggtcatcgaa
    12241 taggcagtag ccggcggggc aattccaaag cccagttcct ggccggtaac ggcatcaatc
    12301 acaatcagtt caagacctcc ttccccaagg tttcgaacaa cccaacaggg attggatggt
    12361 atgggatcca gcggaaagat atccgaatcc ggcgaaatat atactaaacg ggcgccgtcg
    12421 ggatgttgga ctttggccat tgcttcgtga atagagataa tcggcggcag ggtatcggga
    12481 agatcgctgc gccagtgccg gatggtattt ttatgctcgc tggtctggcg gtcaaaatga
    12541 tatacaatct ggtctttttc aacctcggct tgaccgatat aacgctggga atgaaaaacc
    12601 agcaggtcgc cgatttccaa gccccggaag cggtggggct ggggttcttt gatcttcagc
    12661 aggagctcat ttttaactgt ttcggataca tctcgataga gttcggcagc aggcggctgc
    12721 tcggactggt gcaggcgcgt ttgctgagcc gttcgctggt gcatctcctg ccgttcctgt
    12781 tcggttttgg gattgggatc atatacgccg tccggcgagc agcggacgcc taagaattca
    12841 aactgcagat ccttaaacac cccaatatct gtcagatttt tgatgcgttc tccattccat
    12901 acataagcag ggctgccgtc atagattttg tatgtttggg tggtaacagc atactcacgt
    12961 tcaatcagtt catcccgaag cagttcgata gctgtctgaa tatgctcaat ttccgcttga
    13021 gtcagaccga ctccggcagc caattcctgc cacggagcac ggctgccgat tcgtgccagc
    13081 agatacaggt gaaaatattc cggtgcaagg tcgcgaatca tcatctggac gagattttcc
    13141 tggacctctt tctcgccatg cagacaggta tagacaatag tgccatcttg gatagtttta
    13201 tagaaaatat aatgtatctc gatttcaaat gactggccgg ccggcatggc aatgagataa
    13261 ttcagaagcc ccgcctctgc tgcctgcgca aacggacagt agctcattac gaagacatcc
    13321 agatgattat gctggggtaa aacggccgtt gtctgttccg gctcgaggtc caaaccgaac
    13381 gaaaggctga ttaaaagaac ggtgaaaata aaaccgctgc gaatagcttt ggacgctgcc
    13441 ataatcggac tccttgcaaa gaaacaaccc tctctcttct tttgtacccc aataggtaca
    13501 cagtattgca ttatgtgaag cttttactat tttaccactt aagcaggatt aaaatgaaat
    13561 ttttaaaaat atttgtatga agattgtata gcctcacaag gaaaatatat tgtttagcat
    13621 cccttcattc gttttaagcg tattaaaaaa aggagcggaa gattattttt tagttcaaaa
    13681 tctattattt tgaacatttt aatgcgagga aacacaagga attggatgtt tatttgctgg
    13741 aattatcccc aagttaattg ttacaagtta ttcataaaga agaatcctaa aaattgtcaa
    13801 tcaaatgctc tcttgaaata tacaagctga cttggtataa tacttccgtt gtcaataatt
    13861 aataaatcag aatgcataaa atggggttgg ggcatcatgc ggaaaagttt tctcgatata
    13921 gcttatcttg tattaatttt agctattatt atcggctcag cgcttggaga ttgtcctagc
    13981 ggcgatatta caggcgactg tattgtcgac atagatgacc tttttgtagc tgccgaaaat
    14041 tggctgtcca gccaaatgcc cctgcattct ggacttatag cacattgggg atttgatgaa
    14101 caggcaggag aaacggcggc ggattcagca ggggggcata cgggacagct gtatggcgaa
    14161 ccacaatggg tacgcgatga ggagtttcag cgagaggcgc tgatttttga cggcgtggat
    14221 gattatattg aagtgccgga ctacaaaggc attgctggta cagccagccg cacgtgtacc
    14281 gcctggatta agaccagcaa gacaaattct cacattatca attggggttt acagtcacag
    14341 gaagggggaa tgtgggctgt tttagtcggc gataccggcg cgcttcgggt acaagtgagc
    14401 aacggatata tttacggagt taccaatata gctgacggtg agtggcatca tatagcggtt
    14461 gtgcttcaag gtgatggttc gcccgatatc agcgaagcaa tgctgtttgt tgatggactc
    14521 agtgaaacct ccatcggcgg ggttttggcc aagccggtta acacagtcac ttcggaaaac
    14581 gtcaagatag gggtgaatat cctgaaaact gcgtatctgc agggcaggct tgatgaggtg
    14641 cagatttata atcaggcatt gagggaagat gagattctgc gcctttatca aacgggaaat
    14701 gccttgaccc acaacccgga ttttacagga gacggccgcg tggatacaga ggatttcatc
    14761 ccgctgtctg accagtggct tcgggctgag ccggctgtta ttatcagcga gtttctggct
    14821 tccaatgatg ccgctaatcc gccggacatt tcacagggac aaatacgcga cggcaacggg
    14881 gaatcatcgg actggattga actgtataat caaaccgatt ttccgattgc tctgggcggg
    14941 tggggactga ccgacaatgc cggcaatcct cttaaatggc agtttccatc tggaacggta
    15001 ttaagcggac acagttattt aattgtattt gcttctggaa aagagcagag cgattatcca
    15061 agcaattatc cctatgtgga cccgcagggc ttcctgcata ccaattttaa attatccgct
    15121 gacggcgaat atctggctct aacccggccg gacgggaccg ttgaacatgc ttatgattcg
    15181 gtgctcaaca cagtttcggg ccattatgga tttccccctc aggagaaaaa tatttcttac
    15241 gggatgcttc ataatgaaga atactttttt gctttgccat cgccgggaca atccaacagg
    15301 aagtcatttt tgggttttgt ggaggcacca gagttcagcc acgagcgggg tttttacgaa
    15361 acttcttttt ggctgacgct caatacaagc acgcccgatg cggtgattcg ctataccacc
    15421 aacggcacag atccgacgct ttccaacggg acaacctata ccgggcctat tgccgtcagc
    15481 gtttcgacca gtcccgtcgg ccgctgcata cgggcggcgg cattcaagcc tggttatcaa
    15541 gccagtccca tcaaaacccg tacttatcta atgaaggcca ccgagtatat gaaaggactg
    15601 ccggcagtat gtttgtcggg cagcccgaca caggtttttt ataaccccaa cggcattatg
    15661 gcaattgtcg gaggaacatg ggttaatggt gtttggcagc aggttaactc tacggattac
    15721 aataatgtac tcggacacgg gatggactat gagaggcctg tatcgatgga atatattcga
    15781 agaactgacg ggacggagtt tcaggaggat tgcggcattc gtgtccacgg cagcgcctgg
    15841 atgcgtcccc gttatacgat tccatcggtt tcggatgcct ggtatggaac cagcaaatac
    15901 agtcttcgcc tttatttccg cagccgctat ggagacaata cattcgcaca tccgattctg
    15961 gaacaatttc ctgaagtgga tgaaatggat acctttgtgc tccgcgccgg ccacaacgac
    16021 cagaccaatc catttgttcg cgatgaaatg attcgccggc tccagtatca gatgggcaat
    16081 cagacggcgc gagggacatt tgtcaacctt tacatcaacg gtgtgtacaa agggtactat
    16141 aacctctgcg agcggatcaa cgaagattat tgccaaaaat ggttcaacag cgataagccg
    16201 tgggatgtcg tcggctgggt ggcgacaggc tatagttttt atctggaagc ccgcgacggc
    16261 gatacgaagg catttgaaga ttttattaat tatgcaaaaa ataacaactt ggcaaatccc
    16321 ctgtattatg cacaagttga gcaacagatg gatatgccgg cgtttgtgga ttatattatc
    16381 gtgcagacgt ggggcggcaa ctgggactgg ccgcagaata actggacggc ggcagccgaa
    16441 cgttcagctg cccgcaaatg gcgattcttt gtctgggatg cagagggatg catggacggc
    16501 gataccagcc ggaaccgctt cagtaatttg aatacggata actcccctct ttcggtcttg
    16561 tatcgagctc tgagcgtcaa tcaggatttt aagattttgt ttgccgaccg tctgcagaag
    16621 cattttctgg atgccggcgg ggcgatgact caagcgaatc tcaaagccct gttctggcag
    16681 ctggccggcg aggtcaaagg cgttataccc gatatttcaa cctacattcc caatacctat
    16741 attcctgtac gggaaagcat ctattttaca caatgcagaa accaagattt gtttaagttt
    16801 gccgcgccgc gtgtcttttt gtatggccgt gagatacaga aggattatgc tttgcccggt
    16861 gcgcaaataa caatgcaaaa tgccgatggc gtttccggcg atatctatta tactctggac
    16921 gggaaagacc cgcggttgac gctggtagaa cgtgctaaca ccgccacaac gacgctggtg
    16981 gctgaaaatg cctccaagcg ggttttggtt cccacaaagg atatcggaac gacatggaca
    17041 caggtcaatt acgatgattc attgtggaat gatggccttc cggcagacaa ttctaaaacc
    17101 ggcggcgtcg gctatgaaag aaatcccggc gaggcgacca gcactttgcc atatgtcagc
    17161 tacaatgttg aaagcaagat gtacaacaat tacacctcgg catatgttcg cattcccttt
    17221 atagtcgatg caggccaaat gcagagctgg aattatctga gtctgaaaat gcgctatgat
    17281 gacggtttcg ttgcttatat caacggtacg gaggtctgcc gggtcggcat cagcggcccg
    17341 ccggcgtgga attcgacagc accttcacac gaaaacagct cgctggagac atttatcatc
    17401 agcaattatc tgtatcttct tcagccggga ttgaatattc tggctattca cggagcgaat
    17461 tactccaaaa acagcagtga ttttcttgta tctgccattc ttgaggcagg ttatgattca
    17521 gccaacggca ttgctccttc ggcgcagaaa tatactgggc ctatcagcct gtccaaaagt
    17581 gtgcatatca aagcccgaac gctcagcggg acacaatggg gcgcactgcg ggaagctttt
    17641 gtgtctgtgg gaaccatcag cgacaatttg cgcatctcgg aatttttgta taatccagat
    17701 ggtgatccaa acgaggaatt tatcgaactg accaatatcg gcacaactcc tttgaacctc
    17761 aatcaggtca gctttaccaa aggaattcaa tatacctttg gcgatactgt tattaatccc
    17821 ggtcaatatc tgctgctggc tgccgacaag acggtttttg aaaaccgcta tggaaccggt
    17881 cttcctgttg tcggcgagta tgccggtaag ctggacaatg caggggagac cattcaaatc
    17941 agtgatatac tgggcaatcc tatccagacg gttaattata aagacagctg gtatgaaatt
    18001 gccgacagcg acggtttttc gctgacggca gttgatccag cgtatgaaat acccatttca
    18061 agtgacgatc tggctgccca ttggaaattt gatgaaacta cgggcactac cgtctatgat
    18121 atggcagggc ttcatcatgg gacgatttac aatatgcagg ataccgcacg ggtttatggc
    18181 gccaaagaaa atgccctgct ttttgatggg accaatgatt acattgctgt cggcggctat
    18241 aaaggtatca gcggctccgc cagccggaca tgtacagcat ggatcagaac cggtacggtt
    18301 atgtctcaaa taatcagctg gggtgagctc caaacaggcc gcaaatgggc tgtacgcgtc
    18361 aacgatgacg gtacgctgcg ggccgaagtg caggagggat atatttacgg aacgaccccg
    18421 gtagcagata accaatggca ccatgtggca gtcgtactga tcgatgacgg ttcgccggac
    18481 atcagcgagg caattcttta tgtcgatgga aaagctgaga cgactttcgg cggtattcag
    18541 gcccgtgcag ttaataccgc cgctgtcagt gatgttctta tcggagtcaa tctgaacggg
    18601 acggtgttct tccaaggtct tatggatgac ctgcggatat acagccgggc gttgacagct
    18661 gacgagatag ctgcaatgat agcccaggta aactgggaca gcaaggacct ctggcgcccg
    18721 agtgcagttc ggggcggaac gcccggacgc gatgaaacac caaccgaacg agtgccgctg
    18781 ccgggggcga ttgtcatcaa tgagctgctg gctcactccc atgccggcct gcccgactgg
    18841 atagaattat acaatacgac aaatcaggct attgacatca gcggctggtt tttgtctgac
    18901 agcttcagca gccaaacgaa tcgcaagaaa taccggatac ccgatgggac tatcctgact
    18961 ccggctaatc cgtactatgt gctggaagag actgcgttta acaatatctc cgatgccggc
    19021 tgccgcattc cctttgccct gagcgaaggc ggcgacacgc tgtatctcca atcggccaat
    19081 ggagaagaac taaccggcta cttcacaaaa gaagaattcg gggcgaccgc atccggtgtt
    19141 acgctggggc gttttcagaa gagcaccggg acgtggaact tcgtccccat gagtgttccc
    19201 accaaaggtg cagataatgc ttacccgctg gttggtccgg ttatcataac ggagattatg
    19261 tataacccgg ggccaaatcc cattgatcaa gactatgaat ttctggaact gatgaatatc
    19321 agcagtcagc ccgtccgtac ggccagttat atgagtacgt ataccagtcc agacatacat
    19381 atagaagagt ggattccatg gcgatttaca aacggcatcg aatttgaatt tcctttggac
    19441 cttcagctgg agccgggcca gcggattctt cttgttaaaa agcttagcag ttttaacagc
    19501 cgctataccg gtgttccggc aggaaccgtc atttttcaat ggaccagcgg cagcttggct
    19561 aatgaaggcg aaaccctcca gctggcaatg gccggcgatc aggaatacca gaaggaacgg
    19621 tattatatac gtgaagatcg agtcaattat gatgaccaat caccatggcc gactcaggca
    19681 aatggaacag gtctgtctct gactcatctg cggccaatgg aaactggaaa taattacaca
    19741 aatgatcccg t
//
LOCUS       DAOTBO010000046        19417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2313_length_19417_cov_5.704938, whole genome shotgun sequence.
ACCESSION   DAOTBO010000046 DAOTBO010000000
VERSION     DAOTBO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 19417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19417
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_19417_cov_5.704938"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLE88_06855"
     CDS             complement(<1..509)
                     /locus_tag="PLE88_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76219.1"
                     /translation="MFRWICLFGFAAAAAALALHHLVFPCGYNPRFAVRSVVRKLVHL
                     LTLLFLPQTMGWAGRICKLAFLLGLLCFAVLALTGFGPLLCGGRLEGWLLMIHMTAAP
                     VFAVCAAVVVLLAAGRYAFNRADAPAILACAGAGSGRCWLTDSGIGVKIGFWFLAAAG
                     LPLILAPVL"
     gene            complement(509..2347)
                     /locus_tag="PLE88_06860"
     CDS             complement(509..2347)
                     /locus_tag="PLE88_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76220.1"
                     /translation="MGTMFRLLLLAAMAAALLTPAVCADENEKLLGEGNDGNRSRPVH
                     LIELYDHDGNRIRAKDAKPRPFSTKQTCGQCHNYDQIAQGWHFNAHDPQVDSGRPGQP
                     WVLTDVKTRTQIPVTARHWPQTFAPEQVGLSPWDFVNTFFSHFPGGSYGQMPSADPAV
                     AIRKQISGDYEINCLACHNASPQEDQSLAALQAARQNFRWIPTASSGLAVVNGVASSL
                     DDFFDPEFDKGVEITWRTGLFDKDDMVFFDISSKPLDQRCYFCHSNIDLRIGQEAEFT
                     RDDDVHLASGLHCVDCHRNGDDHRINRGYEAQGPGASLTCEGCHLGRDKTGAPEDGRL
                     GAPRPRHAGIPVIHFEKLTCTACHSATWPEEQAGRWKTARIHRVGLHGKHNLDIRQPH
                     IYAPVLMKGTDGKIGPYKLFWPAYWALFENDTVKPFAPKEVLQKARTILDKEVERVDD
                     WRPLTPQEIAEVLALLSTDQKPAVYVAGGKLYRLNDAGELETVAHPAANPYAWPMAHD
                     VRPARQAMGIRQCKDCHTTDSAFFFGKVQIDTPVQIEGGPEFAEMVQLQGINRLYMRV
                     FNFAFVFRPMLKTVAFAACGLIGLVVVAWLLKAAAALTKAFGREED"
     gene            complement(2357..3700)
                     /locus_tag="PLE88_06865"
     CDS             complement(2357..3700)
                     /locus_tag="PLE88_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76221.1"
                     /translation="MNRREFIRTTAALGAGLYVSGKALGADSGSSDVINVALLGAGTQ
                     GQVLMDAVMKAKDPNIRFIAVCDIWEQVNLRKVSRRLKAWESLGYKGTAYTDYKEMLD
                     KEKDLDAVIIATPDFWHSEHACACLAKGLHVYCEKEMSNTIEGARKMVEAQKASGKLL
                     QIGHQRRSNPRYQYCYDKLIRGAKLIGRITTINGQWNRSKASCEDLAAPEGAEIDEAT
                     LNKYGFKNMHQFMNWRWFKGLGGGPIVDLGSHQIDIYSWYLGCNPKTVIASGGIDYWK
                     AHEWYDSVMAVYEYETPDGPVRAMYQTSTTNSANGYYECFMGDRGTLMISESAGRGSV
                     YRENWVLEEEWEPFVKQGLIRKVQTALAEKKDDEAVLDVREGSVAVAEYKIPVTMDVP
                     YHQPHLMNFFNAIRGREKLNCPAEVGFETAVTVLKVNEAVEKACRLEFKPEEFRA"
     gene            complement(3736..5142)
                     /locus_tag="PLE88_06870"
     CDS             complement(3736..5142)
                     /locus_tag="PLE88_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOM76222.1"
                     /translation="MAKITLAILTCLIIGQILAESPAQHADPQAYEGWRLGVQTWSFN
                     RFTLFEAVDKTRSLGLNWIQAYPGQRISDTLTTGFDQNLSPEQRQQVKDKLAESGIGI
                     FAFGVTSIPKDEAAARKLYEFAKEMGIETIVSEPEADQFDLIDKLCAEYSIKLAVHNH
                     PKPSKYWNPDTVLAVCRNRSSWIGACADVGHWVRSGLDPVECLKKLEGRIREVHIKEI
                     DEGHDVIWGTGQGRIKAILEELHRQGYQGTFSIEYEYNWDNNVPEIRQSVAYFNAVGS
                     QLKPTGWKDLFAADLSNADFQEKGWYWDEGVLTVVKGSKDIWTKAQYSDFILDLEFKL
                     AKGSNSGVFLRAGNHEWLPWVEVQVADTFGQPLSRHICGSIYDVKEPTVNAVRPVGQW
                     NRMTLTEKGSRLWVVLNGHLIIDIDLDDWTEPHKNPDGSANKFDIAYKDLPRRGFIGL
                     QDHGTDIWYRNIKIRELN"
     gene            complement(5170..6531)
                     /locus_tag="PLE88_06875"
     CDS             complement(5170..6531)
                     /locus_tag="PLE88_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76223.1"
                     /translation="MKKQLNRRLFLQSSAAAAAFPFFPRTVLGMAPSGSAGSRRLNIA
                     GVGVGGMGQGNLNAVAGTENIVALCDVDWNYAKGTFEKYPQAKRYKDYRVMLEEMGKE
                     IDAVIIATPDHTHACIAMECMKRGKHVYVQKPLTKTVYEARMLTEAARKYGVITQMGN
                     QGHSDEGIRLICEWIWDGAIGDVREVHAWTNRPVWPQGLDRPSETPPVPDTLDWDLWL
                     GPAPYRPYHPAYHPWNWRAWVDFGTGALGDMACHILDPVFWALKLKYPVSVQGSYVAN
                     VIEKWKKQDVAESYPLGSIIHLDYPAREGMPAVKVHWYDGGLMPERPEELEEGRRMGD
                     NDGGVLFVGDKGKLMCGCYGKAPQLIPYSRMQEYKRPPKTLPRVQTSHEMNWVNAIKE
                     GKKASCDFDYAGPFTEMVLMGNLCLFKPGQKILWDGETMKSPNMPDLDKYVNPPYREG
                     WHL"
     gene            complement(6548..9370)
                     /locus_tag="PLE88_06880"
     CDS             complement(6548..9370)
                     /locus_tag="PLE88_06880"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOM76224.1"
                     /translation="MKRLIDFVWLLVLMAAMTGYAVTEQEKQAIQSAMPDQPVVKPAA
                     ERTMLVFSLCNGFKHSSIPYWIEALNVMSQKTGAFKVVHSTEMSVFNPETLRQYDAIC
                     FNNTTKLTPDAAQQKAILDFVKSGKGIVGIHAATDNFYDWPEGMEMMGGVFSGHPWTG
                     DGTWAVKIDDPQHPLMQPFKGQGFKIKDEIYRTAAPLYSRTKQRVLMSLDMSDPATKN
                     ARGVTPEDADTGISWIKQIGQGRLFYCSLGHDHAVTWNPAVLQHYLAGIQYALGDLKV
                     DDRPLAKTLEAEKVDAFIEQLRQYDWDKPKAPLYAFSELIREYGDAANLKGLLEAKLL
                     QVLEADVPLAAKDYICRQLAVIGSGEAVPVLGKMLDASETSDMARYALERIPSDLADK
                     LLLSRLQMASSPTAQIGIINSLGVRRTDAAAAVLAEYAGSANPAVALAAIQALSAVGS
                     RSAAETLQKLTVDEALQERLADALLACADSLCRQGHLSDAQAIYKKLYASGRSAAARI
                     GALNGLAKTNAAEMRTILPEAIKGQDLVLQAAAIQILAGIEDASLLEEAAAQMDTLPD
                     NAKIQLCAALSANPKKIGRDKIERLVGSPEKEVRIAAYQALAVLGNASSVEPLAKAAA
                     AAADRQEAQQARAALYLLSAPEVNKVIIDRIESLVSAGDEKTVVELIRAAGQRGLSEA
                     VPALLRTARGEGDAASESVRVLQGLAGLKDMGEFADLVTEKPLSRNADALAAAARRLD
                     DAAGCTAAVLARYDSAASEEAKVTLLRVLGKLGDAKAAALLRKEKDAPSQTLRQGALR
                     AMMDWPGGDFIDEMKTLAEQEQDQRTRVMAFTAYVRMITETSDKNSDAGVGALIEAFS
                     MAPQPDQQRLVIGALGSFVSQKALDFTARFLDNSALKAEAQASVVRICEAGGISKLPQ
                     AKDLLAKVAAAAENRNLKERAEKLLR"
     gene            complement(9414..10727)
                     /locus_tag="PLE88_06885"
     CDS             complement(9414..10727)
                     /locus_tag="PLE88_06885"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76225.1"
                     /translation="MNKKEISRRTFLKTSTAVGASIVGFPYLIRSSALGLAGTVAPSN
                     RLVTAQIGCGSMGTGDLQSLLNFAPAVQVVAVCDVDDNHSANAKRLVDQKNGNADCRV
                     YRDYRQLLENHTLDIVSHALPDHWHAIVSVACARKGIDMYGQKPLARTIREGRAICDA
                     VKRYGIIWQTGSWQRSVSNFHQAAELVRNGRIGKVSYVEVGLPNGGRGPRYRQLEVPA
                     AFDWQMWLGPAPWRPYQDFGRGNVHWDWRWIMDYSGGQLTDWAGHHVDIAHWGLGLDY
                     SGPVEVEGKGEYPTEGIYDVPMAYDITCTYSTGLTMRIANEAKLPHRMGTCWYGDGGW
                     IHVTRGGLSASNPKILQETIGPDEIHLEKSEDHFKNFLDCVRTRRTTITPAEIAHRSI
                     SVGFLGEIAMLTGRKLKWDPQQELFVGDAEANRLLERSYRAPWHL"
     gene            complement(10816..11808)
                     /locus_tag="PLE88_06890"
     CDS             complement(10816..11808)
                     /locus_tag="PLE88_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOM76226.1"
                     /translation="MARPVTIFTGQWADLPLEKLAELMAGIGYDGLELACWGDHLDVF
                     KAAEDLAYCKKQKAILEKHHLKLFAISNHLAGQLVCDLNNDSRSDMFAPADCAGNAEK
                     KRQWAVKAMKATARAAKNMGVQVVNGFTGSSIWHLLYSFPPVSDAMIDEGFKFFADMW
                     NPILDVFDECGVKFALEVHPTEIAFDLYTAKRALEAIGNRKTFGFNFDPSHLHWQMVD
                     PVRFLKEFPDRIYHTHMKDAALQLDGRSGILASHLNFGNPNRGWDFRSLGHGGVNFEE
                     IIRTLNHIGYNGPLSVEWEDCGMDRIHGATEALQFVRKTDFSPSNVQFDAAFDK"
     gene            complement(11844..13016)
                     /locus_tag="PLE88_06895"
     CDS             complement(11844..13016)
                     /locus_tag="PLE88_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76227.1"
                     /translation="MTLDRTLKMGMVGGGPGAFIGEVHRKAARMDGGIEIVGGAFDVD
                     PRKSKQMGRQLLLNPRRVYRDYREMIDKELRLPEGERMDFVAVCTPNNWHYPIAKDLL
                     EAGFHVMCEKPMTVSVQQARELVKIVQKTRRVFGLMHNYTGYPMVKLARDMVRSGRLG
                     KIRKIVVQYPQGWLATALEKTGQQQASWRTDPKQSGASGCGGDIGTHAENLSEYITGL
                     KMTELCAELTTFVKGRKLDDDVNCLVKFNNGARGLLHASQVSVGEENNLAIWIYGETG
                     GLEWHQEHPNYLYVTPMGQPTQVWKRGNPYVAQASPAAARATRLPSGHPEAFIEAFAN
                     IYRNFADTLRARLTHTKADPIITDFPDVNDGLRGMLFLETLVASAASRTKWTKFKK"
     gene            13293..14345
                     /locus_tag="PLE88_06900"
     CDS             13293..14345
                     /locus_tag="PLE88_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOM76228.1"
                     /translation="MPVKLLQQPQPAIFYDVDGTHKADTCENLKAASKRGELHLGGLA
                     HGCYPGVKMPPKMLPELCVACFWDAHKDQSWGLPMHRNEGIELGYLTRGRLDFIVDGV
                     HYKLKGGDLTITRPWQPHQVGNPLISASRMHWLILDVNVRRPNDPWKWPPWINFAAHD
                     LQKLTQLLSHNEQVVWQGNKQIEKCFEQIAEHIQNAEKPESVQTRLQLYINELLLEVF
                     EMLQTRNIKLNARLSSTHRSVEMFLAGLSEHLEYPWTLESMARHCNLGRSRFSHYCRQ
                     ITNMTAADYLIHCRIERAKKMLSESNGKTILDIAMACGFESSQYFATCFKKKTGTTPT
                     QYRTDVLSGKQQRNVL"
     gene            14582..15433
                     /locus_tag="PLE88_06905"
     CDS             14582..15433
                     /locus_tag="PLE88_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76229.1"
                     /translation="MSYNSFGRKEGFTLIELLVVIAIIALLLSIIIPALGKAKMQVEE
                     ILCRSNLHQYHIATELYTTEEDDTYPNPWVSLYKEYQFPDEIQRYCRWHNPLYNLDSY
                     GDRYGGPYWSYLSATKANICPTFRKYAEQFGQFHMGGDQAGLTCIGQPFVSQFSYSMN
                     GILGDQRNFKGIKKSQIASSPSQTFLWGEENMWPLYDADGSTKLSRYVLNDTALLTPG
                     DCFGSFHKVSKGQLSAQMPQTARGYGTYNGGVSNVLMMDGSSASLSPAGGQTLQYAGR
                     IRSGGTQ"
     gene            complement(15464..15640)
                     /locus_tag="PLE88_06910"
     CDS             complement(15464..15640)
                     /locus_tag="PLE88_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76230.1"
                     /translation="MAKCPYCEKTITLKKGEQNQIRRQTEGVVKKEVLYSCPHCETVL
                     GFGFFLGGLVTGQP"
     gene            complement(15667..16203)
                     /locus_tag="PLE88_06915"
     CDS             complement(15667..16203)
                     /locus_tag="PLE88_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76231.1"
                     /translation="MTKREIASLVLKLMGVFILLKTIGYVPTVFGVLFTTGRYNSNAG
                     FASLALQVMGMLLLLAFALGWAVLIIVLSDKFAKWLIKEDKSLEIAHPISKEDVMVVA
                     VSCIGLYLIVAAIPSIMHHLYNFLLLHRPDGNYPPYWSGGFSRILTLISPLVQIALGV
                     WLFAGSRGIVKLWKRIRG"
     gene            complement(16215..17120)
                     /locus_tag="PLE88_06920"
     CDS             complement(16215..17120)
                     /locus_tag="PLE88_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOM76232.1"
                     /translation="MWGWVTNDCSVLAELSVSAVSREIADAVAEQLAVDLVQEPGRLV
                     IVLRKPQEHKDNYCVSGRMTITVPLETRAAITTTYGDCRAENITRQVAMQSTHGDILF
                     DNIRGDIVAVSTYGDIKLANTQNASVNATTTHGGIKLDNSNIRQVRCSTIYGPIRLKA
                     ILADRLDLQTSQDSIELTACTAKEAELNTSYGAIGGDIGGIERITARTSHAPIDLRCV
                     NESSPNLAATLSTTYNNIAFCPPAGFAGRVSASTSYGHIRTDRSIVVQGVLGEQLNGT
                     IGEGTGSITLTTTHGNIDLKNPNPQ"
     gene            complement(17512..18255)
                     /locus_tag="PLE88_06925"
     CDS             complement(17512..18255)
                     /locus_tag="PLE88_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76233.1"
                     /translation="MGKVIIPRLLPLIFIIEYGLYLYLKNVLSDEIEIKARLLKIFLG
                     IFLFGAGGLLYMFVIYPYVIRYLGGSHQITQKWFNYADKFIYWKRVMGYWIEASEEFP
                     GLKKLIFKTRQRIHALWLPEDQTLHPAIIDFIDSHAQQIEPSYPKPIPVLSNGQWYFC
                     LSFIFIFSVAAAILIENSPFLQSHAIVVFFVLLLFPGTISLVFALGKEFFKDKYLPFA
                     LVLNWSAFMVFVIIIVLYEFYKYHKIIDG"
     gene            complement(18748..19047)
                     /locus_tag="PLE88_06930"
     CDS             complement(18748..19047)
                     /locus_tag="PLE88_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76234.1"
                     /translation="MINGDAAGSAVGVVYAAMTDAIKAMGVIVQVEPHVFEQLLRLQA
                     ASVPLVVFSPVGFFKKNQYLTAYKGLVFYTKTSTALTLPAYVELIQAKKIWIPNM"
     gene            complement(19073..19297)
                     /locus_tag="PLE88_06935"
     CDS             complement(19073..19297)
                     /locus_tag="PLE88_06935"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOM76235.1"
                     /translation="MANGVSEKRGIACLLFLLLLGPLGIHRFYVGKAGTGVLFLLTCG
                     GLGLWWLIDLILLVAGSFTDADGNTVKLSK"
BASE COUNT         4597 a         5465 c         4591 g         4764 t
ORIGIN      
        1 ctcagcaccg gcgccaatat cagcggcaat ccagcagcgg ctaaaaacca gaaacctatt
       61 tttactccaa taccgctgtc ggtcagccag cagcgtccag accctgcccc agcacaggcc
      121 aaaattgcgg gcgcatcggc cctgttaaag gcgtatcgtc ctgcagccag cagtacaacc
      181 actgccgcac aaacagcaaa aaccggcgcc gccgtcatat gaatcattag aagccatcct
      241 tccagacgtc cgccgcataa aagcgggccg aaacccgtca gcgccagcac cgcaaagcaa
      301 agcagcccga gaagaaacgc cagtttgcag atgcgtccgg cccagcccat cgtttgcggc
      361 agaaacagca gtgtcaggag gtgcacaagt ttgcgaacca cagaccgtac agcgaaacgg
      421 gggttatagc cgcaggggaa aaccagatgg tgcagtgcca gcgccgcagc tgcggcggca
      481 aatccaaaca gacaaatcca tcgaaacatc agtcttcctc cctgccaaat gccttcgtca
      541 gggccgctgc tgctttaaga agccaagcca ctacgactaa tccgatcaat ccgcaggcgg
      601 caaaggcaac tgttttgagc atcggccgaa atacgaaggc aaagttaaat acccgcatat
      661 acagccggtt gatgccctga agctgaacca tttctgcaaa ctccggcccg ccttcgattt
      721 gaaccggtgt atcaatctgc acttttccga agaaaaaggc cgaatcggtc gtgtggcagt
      781 ctttgcattg gcgaatcccc atcgcctgcc gggcgggtcg gacatcatga gccatcggcc
      841 aggcataggg gttggccgcc ggatgggcaa ccgtttccag ttcgcccgcg tcattgagac
      901 gatagagttt cccgccggca acataaacgg cgggcttctg gtctgtactt aacagagcca
      961 gaacctcggc aatttcctgc ggagttaaag gccgccagtc gtccacccgc tcgacttcct
     1021 tgtccagaat agtacgggct ttctggagta cctctttggg agcaaagggc ttgacagtat
     1081 cgttttcaaa cagtgcccaa taggccggcc agaataattt ataggggccg attttaccat
     1141 ccgttccctt catcaaaacc ggtgcataga tatgcggctg gcgaatgtca aggttgtgtt
     1201 ttccatgcag accaaccctg tgaatacggg ctgttttcca ccgtccggcc tgttcctccg
     1261 gccaagtagc cgaatggcag gccgtgcagg tgagtttttc aaaatgaatg acgggaatgc
     1321 cagcgtgccg cggccgcgga gcgccaagcc ggccatcttc gggggcccct gttttatccc
     1381 tgcccaaatg acagccctca catgtcagcg atgcgccggg gccttgggct tcatatcctc
     1441 gattaatccg gtggtcatcg ccgtttcgat gacaatctac gcaatggaga ccggatgcca
     1501 aatgcacatc gtcgtcgcga gtgaattcgg cctcctgtcc aatccgtaaa tcgatattgg
     1561 aatggcagaa atagcatcgc tggtcaagcg gttttgagga aatgtcgaaa aagaccatat
     1621 catctttatc gaacagaccc gtccgccacg tgatttccac gcctttgtca aattcgggat
     1681 caaaaaaatc atccagcgag gaagccaccc cattgacgac ggccaatccg ctggaggccg
     1741 taggaatcca gcgaaagttc tgacgggcgg cctgcagggc ggccaagctc tggtcttcct
     1801 gcgggctggc attatgacag gccaggcagt taatctcata gtccccgctg atttgcttgc
     1861 gaatcgccac ggccgggtcg gctgaaggca tctgcccata gctgccgccg ggaaaatggc
     1921 tgaaaaatgt gttgacaaaa tcccacggcg aaagccctac ttgttcggga gcgaatgttt
     1981 gcggccaatg acgggctgta actggtatct gggtgcgggt tttgacatct gtcagcaccc
     2041 acggctgtcc ggggcggccc gaatcgacct gcggatcatg ggcattaaaa tgccagccct
     2101 gagctatttg gtcatagttg tggcattggc cgcaggtctg cttggttgaa aacggtctgg
     2161 gctttgcgtc cttggcacga atccggttgc cgtcgtggtc gtataactca attaaatgca
     2221 ccggccggct tcggttgccg tcgttgcctt cgcccagcag tttttcgttt tcgtcagcac
     2281 aaacggccgg agtcaataaa gctgcagcca tcgccgccag cagcagaagt cggaacatcg
     2341 tacccattcc tgatccttac gcacggaatt cttccggctt gaattcaagc cggcaggcct
     2401 tttcaaccgc ttcattaacc ttcagcacgg taactgccgt ttcaaagccg acctctgccg
     2461 gacagttgag tttttcccgc ccgcgaatgg cattaaaaaa gttcatcaga tgcggctgat
     2521 gatacggcac atccatcgtc acagggattt tgtattcagc taccgccaca ctgccttccc
     2581 ggacatccag cacggcttca tcgtctttct tttctgccag ggctgtctgg accttgcgaa
     2641 tcagaccctg cttgacaaac ggttcccatt cttcttccag cacccagttt tcgcgataca
     2701 cactgccgcg gccggcagat tcggaaatca tcagcgttcc acggtcgccc ataaagcatt
     2761 cataatagcc gttggcgctg ttggtggtgg atgtctggta catcgcccgc accggtccat
     2821 cgggtgtttc atattcatag acagccatca cgctgtcgta ccattcgtga gctttccagt
     2881 agtcgatgcc gccgctggct ataactgtct tcggattgca gcccaaatac cagctgtaaa
     2941 tatcaatttg atgactaccc agatccacta tcgggccgcc gcccaagccc ttgaaccatc
     3001 gccagttcat aaactggtgc atattcttaa agccgtattt attcagcgtg gcctcgtcaa
     3061 tttcggctcc ttcaggggca gcgaggtctt cgcaggaggc cttgctgcgg ttccactgac
     3121 cattgatggt tgtgatgcgg ccgatgagct tggcaccgcg gataagcttg tcgtagcagt
     3181 attgatagcg gggattgctg cgccgctggt gaccaatctg cagaagcttg ccgctggcct
     3241 tctgggcttc aaccattttg cgggcgcctt caatggtatt gctcatttct ttttcgcagt
     3301 acacatgcag acccttggcc agacaggcac aggcatgctc cgaatgccag aaatccggcg
     3361 tggcaataat caccgcatcc aaatcctttt ccttatcgag catttctttg tagtcggtat
     3421 aggcggttcc tttgtatccc agactttccc atgccttcag ccggcgggat accttgcgga
     3481 gattgacctg ctcccaaata tcgcagacag caataaaccg aatattcgga tctttggcct
     3541 tcatcactgc atccatcagc acctgtccct gcgtgccggc gcccagcagt gccacattaa
     3601 tcacatcact gctgccggaa tctgccccca gtgcttttcc gctgacatac aatccggcac
     3661 cgagtgccgc ggttgtgcga ataaactctc tgcgattcaa tcctttttgc ataacacctt
     3721 aaacctttct gtttatcagt ttagttctct gattttgata ttgcgatacc aaatatccgt
     3781 cccgtgatcc tgcaggccga tgaatccccg gcggggcaga tctttatagg cgatgtcaaa
     3841 tttattggcc gagccgtccg gatttttgtg cggctctgtc cagtcgtcaa ggtctatatc
     3901 gataatcaga tgaccgttaa gcacaaccca cagacgcgat cctttttcgg ttagggtcat
     3961 tcgattccac tggccaacag gccggacggc attgaccgtc ggctctttga catcataaat
     4021 acttccgcag atgtgccggc tgagcggctg gccgaaggta tctgctacct gcacttcaac
     4081 ccacggcagc cattcatgat tgccggccct cagaaaaaca ccgctgttgc tgcctttggc
     4141 cagcttgaat tccaaatcca aaataaaatc gctgtattga gcctttgtcc agatgtcttt
     4201 gctgccctta accactgtca ggacaccttc gtcccagtac cagccctttt cctgaaaatc
     4261 tgcattggaa aggtctgccg caaacaggtc tttccatccg gtcggcttta attgggagcc
     4321 aacagcatta aaataggcca ctgattggcg aatttcaggg acattgttgt cccagttgta
     4381 ttcatactct atcgaaaaag tcccctgata cccttgtctg tgcagttctt cgagaatcgc
     4441 cttgatgcgt ccctgcccag taccccaaat aacatcatgg ccttcatcga tttccttgat
     4501 atgaacttcg cgaatccggc cttcaagttt tttaagacac tccaccggat ccaagccgct
     4561 tcgcacccaa tggccaacat cagcacaagc cccaatccaa ctgcttcggt tccggcagac
     4621 agccagcacg gtgtccggat tccagtattt cgaaggtttg ggatgattat gcactgccag
     4681 cttaatgctg tattcggcac agagtttgtc tatcaagtca aattggtccg cctcgggttc
     4741 cgaaacaatc gtctcaatcc ccatttcttt ggcaaattca tagagcttgc gggcggcagc
     4801 ctcatctttg ggaatgctgg tcacgccgaa agcaaaaatg ccgatgcctg attccgccag
     4861 tttgtcttta acctgctgcc gctgttcggg tgaaaggttc tggtcaaaac ctgtcgtcaa
     4921 agtatcggaa atccgctgac ctggataggc ctgaatccag ttaagtccca gtgaacgggt
     4981 tttatcaacc gcctcaaaca gcgtaaaacg gttaaacgac caagtctgca cacccagccg
     5041 ccatccctca taggcctgcg ggtcagcgtg ctgtgccggc gattcggcta aaatctgtcc
     5101 gatgatcagg caagtaagga ttgccagtgt tattttggcc attggtagtt tccttttctg
     5161 ttattgtttt tagagatgcc acccttcccg atacggcggg ttgacatatt tatcaaggtc
     5221 cggcatattc ggcgacttca ttgtttcgcc gtcccagaga atcttctggc cgggcttgaa
     5281 caggcacaga ttgcccatca gcaccatctc ggtaaacggg ccggcatagt caaaatcgca
     5341 gctggccttt ttgccttctt tgatagcatt gacccagttc atctcgtggc tggtctgcac
     5401 ccgcggcagg gtttttggcg gccgcttgta ttcctgcatc cggctgtagg gaatcagctg
     5461 cggggctttc ccatagcagc cgcacatcaa tttgcccttg tcgccgacaa acagcacgcc
     5521 gccgtcattg tcgcccatcc gccgcccttc ttccagctcc tccggccgtt ccggcatcaa
     5581 tccgccgtca taccagtgca ctttgaccgc cggcatccct tcccgtgccg gatagtccag
     5641 atgaatgatc gagcccagcg gatacgattc ggcaacatcc tgctttttcc acttttcaat
     5701 tacattggct acataactgc cctgcacgct gacgggatac ttaagcttca aagcccagaa
     5761 tacggggtct aaaatatggc aggccatatc gcccagtgct ccggttccaa aatctaccca
     5821 cgctcgccag ttccatggat gatacgccgg atgatagggt cggtatgggg ccgggcccag
     5881 ccataaatcc caatcaaggg tatccggcac aggcggcgtt tccgacggcc ggtccaaacc
     5941 ctgcggccag accggacggt tggtccaggc atgcacttcc cgcacgtcac cgatggcgcc
     6001 gtcccaaatc cattcgcaaa tcaggcggat gccttcgtcg ctgtgtccct gattgcccat
     6061 ttgggttatc actccgtatt tgcgagccgc ttccgtcagc attcgcgctt catagaccgt
     6121 cttggtcagg ggtttttgga catagacgtg cttgccgcgc ttcatacatt ccattgcaat
     6181 gcaggcatgg gtatggtcag gcgtagcaat gataaccgca tcaatctcct tgcccatctc
     6241 ttcgagcatc acacgataat ccttataccg cttggcctgc ggatacttct caaatgttcc
     6301 cttggcataa ttccagtcca catcgcacaa agcaacgatg ttctctgtgc cggctactgc
     6361 attcagattg ccttgcccca ttccgccaac gcccaccccg gcaatattca accggcggct
     6421 gccggcagag ccggacgggg ccatacccaa aacggtgcga ggaaaaaaag gaaatgcggc
     6481 agcggcggcc gacgattgca aaaaaagtct gcgattcaat tgctttttca taattccatc
     6541 ctcatggtta ccttaaaagt ttttccgcac gctctttaag atttctgttt tctgccgcag
     6601 cagcaacttt tgcaagaaga tcttttgcct gcggcagttt gctgattccg ccggcctcac
     6661 agatgcgcac gacgctggct tgggcttcgg ctttcagagc cgaattatcc agaaaccgtg
     6721 cagtaaaatc aagggctttc tgcgacacga aggaccccag tgccccgatt acgagccgct
     6781 gctgatcggg ctgaggtgcc attgaaaagg cctcgatcaa cgcaccgaca cctgcatcgc
     6841 tgtttttatc tgaggtttcc gttatcatcc gcacgtaagc cgtaaaagcc ataacccggg
     6901 ttcgttggtc ctgctcttgt tcggccagcg ttttcatttc gtctataaag tcgccgcccg
     6961 gccaatccat catcgcccgt agcgcccctt ggcgaagcgt ctgactgggt gcatcttttt
     7021 ccttcctcag cagtgcagcg gctttggcat ctccgagctt gcccagaacc cgcagcagcg
     7081 ttactttggc ctcttcactg gcggcggagt cataacgggc aagaacagcc gccgtacaac
     7141 cggcggcatc atcaagccgc cgcgccgccg ccgccagtgc gtcggcattg cgggacaatg
     7201 gcttttctgt cactaaatct gcaaattcgc ccatatcctt cagcccagcc agtccctgca
     7261 gcacacggac agattccgat gccgcatcgc cttccccgcg ggccgtccgg agcaatgccg
     7321 gcaccgcttc ggacaggccg cgctggcccg ctgcccgaat cagttcaacc accgtctttt
     7381 catcgccggc acttaccagc gattctatac ggtcgataat tactttgttg acctcgggag
     7441 cactgaggag atacagcgca gctcgtgcct gctgtgcttc ctgacggtcg gcggctgcgg
     7501 cagctgcctt tgccagcggc tcaacagatg atgcgttccc cagcacagcc agtgcctgat
     7561 aggccgctat gcgaacttct ttttcaggac tgccgacaag ccgctcgatt ttgtcccggc
     7621 cgattttttt tggattagca gacagcgctg cacagagctg tatcttggca ttgtccggca
     7681 gcgtatccat ttgagctgct gcctcttcca gcagtgaagc atcctctatg ccggccagta
     7741 tctgaatcgc cgctgcctgc aaaacgaggt cttggccttt tatggcttcc ggcagaattg
     7801 tgcgcatttc cgcggcattg gttttagcca gtccgttcag agcgccgata cgagctgcgg
     7861 ctgaacggcc ggaggcatat agttttttat aaatcgcttg ggcatcggac aagtgcccct
     7921 gccggcacag ggagtcggca catgccagca aagcatcggc cagacgctct tgaagcgctt
     7981 catccactgt cagcttctgc agtgtctcgg cagcactgcg gctgccgaca gcactcagcg
     8041 cttggatagc cgccagagca accgccggat tcgcgctgcc ggcatattcc gcaagcactg
     8101 cggccgcagc gtctgtccgg cgcacaccca gcgaattgat gatgccgatt tgtgctgtcg
     8161 ggctggatgc catttgaagc ctgctcagca gcagtttatc agccaaatca gaaggtatcc
     8221 gctccagcgc atagcgtgcc atgtcggaag tctcggatgc atcaagcatc ttccccaata
     8281 ccggcactgc ttcaccagat ccaatcaccg caagctgccg gcagatataa tccttggccg
     8341 ccagcggcac atcggcctcc agtacctgaa gcagcttggc ctctaacagc cctttgaggt
     8401 tcgctgcatc accgtattct ctgatgagtt cggaaaacgc atacagcggc gccttcggtt
     8461 tatcccagtc atactgcctg agctgctcaa tgaaggcatc caccttctca gcttccagcg
     8521 ttttggccag cggcctgtca tcgactttca ggtcgcctaa ggcatactga attccggcca
     8581 gataatgctg cagcacagct ggattccacg ttaccgcatg gtcatgcccc aacgaacaat
     8641 aaaacagccg cccctgacct atctgcttga tccagctgat gccggtatcg gcatcttccg
     8701 gtgttacacc gcgtgcgttt tttgtagccg gatctgacat atccagtgac atcaaaacac
     8761 gctgcttggt gcgggaatac agcggtgcag ctgtgcggta aatctcatct ttgatcttaa
     8821 agccctgccc tttgaagggc tgcatcagcg gatgctgcgg gtcatcaatt ttaaccgccc
     8881 atgtgccatc gcccgtccag ggatgaccgg aaaagacgcc tcccatcatc tccattcctt
     8941 ccggccaatc gtaaaaatta tccgtcgcag cgtgtatgcc cacaattccc ttgccgctct
     9001 tgacaaaatc taaaatcgct ttctgctgcg ctgcatccgg cgtcagcttg gtggtattgt
     9061 taaagcagat ggcgtcgtac tgccggagcg tttccggatt gaacacactc atctcggtgc
     9121 tgtgaaccac cttaaaagcg ccggtctttt ggctcatcac attcagcgct tcaatccaat
     9181 aaggaataga gctgtgcttg aatccattgc acaagctgaa taccagcatc gttcgctcag
     9241 cagcgggctt gacaacaggc tgatccggca ttgcagactg gattgcctgt ttttcctgct
     9301 ctgttaccgc atatccggtc attgctgcca tcagcaccaa aagccataca aaatctatca
     9361 atcgtttcat acaggcctca ccgtcaatat tctgttaaac taaatttccc ctgctataga
     9421 tgccacggcg cccgatagct gcgttccagg aggcggtttg cttcggcatc gccgacaaac
     9481 agctcctgct gcggatccca tttgagcttg cggccggtca gcattgcaat ttcgcccaga
     9541 aagccgacgc tgatggaccg gtgggctatt tctgccggcg taatcgtcgt gcggcgggtt
     9601 ctgacgcagt cgagaaagtt tttgaagtgg tcttcgcttt tttccaaatg aatctcatcc
     9661 gggccgattg tttcctgaag aattttcgga ttcgaagccg acagaccgcc tcgggtgaca
     9721 tgtatccagc ccccgtctcc gtaccaacaa gtgcccattc tgtggggcag cttggcctca
     9781 ttggcaatcc gcatcgtcag accggtcgaa taggtgcagg taatatcata ggccatcgga
     9841 acatcataaa tcccttcggt cgggtattcg cctttgccct caacctcaac cggaccgctg
     9901 taatccagcc ccagccccca gtgggcaata tctacatggt gacctgccca gtcggtcaac
     9961 tggccgccgg aataatccat aatccaccgc cagtcccaat gcacattgcc ccgtccgaaa
    10021 tcctgatacg gacgccacgg tgctggtccc agccacatct gccagtcaaa agcagccggc
    10081 acctccagct gtcggtatct gggtccccgg ccgccgttag gcagacccac ttccacatag
    10141 ctcaccttgc cgatgcggcc gtttcggacc agctcggcgg cctgatgaaa attgctcacc
    10201 gaccgctgcc agctccccgt ctgccagatg attccatagc gcttgactgc atcgcaaatc
    10261 gcccgtcctt cccgaatggt tcgcgccagc ggcttctgtc catacatatc aatgcccttg
    10321 cgggcacagg ccactgaaac aatcgcatgc cagtggtcag gcagcgcatg gctgacaatg
    10381 tccagcgtgt ggttttccag aagctggcga taatcacggt aaacgcggca gtccgcattg
    10441 ccgttttttt gatcgactaa gcgcttggcg ttggcactgt ggttatcgtc cacatcgcag
    10501 acagccacta cctgtacagc cggagcaaaa ttcagtaacg actgcaggtc ccctgttccc
    10561 atagaaccgc agccaatctg tgccgtaacc agccggttgc tcggggctac agttccggca
    10621 agtcccaaag ccgatgaccg tatcagataa ggaaatccca caatgctggc cccaacagcg
    10681 gtactggttt tcagaaatgt ccttcggctt atctcttttt tgttcatata catcctcttg
    10741 ttttgcctaa atacatccgc aaagtacagc acagatggta taaccgttct gagtccggtt
    10801 ttatttcaat gggccctatt tatcgaaggc cgcatcaaac tgcacattcg acggcgaaaa
    10861 atcggttttc cggacgaatt gaagggcttc tgtcgcaccg tgaatgcggt ccattccgca
    10921 atcttcccac tcgaccgaca gcggcccgtt atagccgatg tgattgagcg tgcggatgat
    10981 ctcttcaaaa ttcaccccgc cgtgtcccag cgagcggaaa tcccaaccgc gatttggatt
    11041 gccgaagttt aaatgcgaag ccagaatccc gctgcgtcca tccagctgca aggccgcatc
    11101 tttcatatgc gtatggtaaa tgcggtcagg aaactccttg agaaaacgaa ccggatccac
    11161 catttgccaa tgcagatgac tgggatcaaa attaaagccg aaggtcttgc ggtttccgat
    11221 ggcctccaag gcccgcttgg cggtatatag gtcaaaggcg atttccgtcg ggtgcacttc
    11281 caacgcaaat ttcacgccgc attcatcaaa tacatccagt atcggattcc acatatccgc
    11341 aaaaaactta aaaccctcgt caatcattgc atcgctgacc ggcggaaaac tatacagcag
    11401 atgccagatg ctcgaaccgg taaatccatt gacgacttga acacccatat ttttggcggc
    11461 tctggcggtg gccttcattg ccttgacggc ccactgacgc tttttttcgg cgttgccggc
    11521 acaatcggca ggagcaaaca tatccgaacg gctgtcattg ttaaggtcgc agacaagttg
    11581 gccggccaga tgattgctga tggcaaacag cttgagatga tgcttttcca aaatagcttt
    11641 ctgcttttta cagtaggcca aatcttcggc ggctttgaac acatccagat ggtcgcccca
    11701 acaggccagc tccaaaccat catacccaat gccggccatc agttcggcca gcttttccag
    11761 cggcagatcg gcccattggc cggtaaaaat tgttacaggt cgtgccataa ttttatctcc
    11821 ttgctcttaa aagcataagc ggattacttt ttgaactttg tccattttgt ccggctggca
    11881 gcacttgcaa ccagcgtctc cagaaacagc attccccgca ggccgtcgtt gacatcgggg
    11941 aaatctgtaa taatcgggtc agctttggta tgcgtcaggc gtgctcgaag ggtatcagca
    12001 aaattgcggt agatgttggc aaaggcctcg atgaaggctt ccggatgacc agacggcaga
    12061 cgggttgcac gggcagcggc aggactggct tgggcgacat agggattacc ccgcttccag
    12121 acctgcgtgg gctggcccat cggggttaca tacagataat tcgggtgctc ctgatgccat
    12181 tccaagccgc cggtttcgcc gtaaatccag atagccagat tgttttcctc gcccacagag
    12241 acctgactgg catgcagcag gccccgagct ccgttgttaa acttgaccag acagttgaca
    12301 tcatcatcca gcttgcgtcc cttgacaaag gtggtcagct cggcgcacag ctcggtcatc
    12361 ttcagaccgg tgatgtattc cgagagattt tcggcatggg tgccgatgtc gccgccgcag
    12421 ccggaagcgc cgctctgctt ggggtcggtc cgccacgagg cctgctgctg gcctgtcttt
    12481 tccaatgcgg tggccagcca gccctgcggg tactgcacca caatcttgcg aatcttgccc
    12541 agacggccgc tgcgcaccat atcgcgggcc agcttgacca tcggataacc ggtataattg
    12601 tgcatcagcc caaaaacgcg gcgggttttc tgaacgattt tgaccaactc gcgggcctgc
    12661 tgcactgata cggtcatcgg cttttcgcac atcacatgaa agcccgcttc cagcaggtct
    12721 ttggcaatcg gataatgcca gttgttgggc gtgcagacag ccacaaaatc catacgctcc
    12781 ccttccggca aacgcagttc cttgtcaatc atttcgcgat aatccctata cacccggcgg
    12841 ggattgagca gcagctgccg gcccatttgt ttggatttgc ggggatcgac gtcaaaagcc
    12901 cctccgacga tttcgatgcc tccatccatc cgtgcggcct tgcgatgcac ttctccgata
    12961 aatgcccccg gaccccctcc gaccattccc atcttcaatg tcctgtcaag agtcatacgg
    13021 cttcctttcg caaaatgaag ttgtcagtta tctcctccat ccccaacgat ccgccctgag
    13081 gatgctgcat cctatcactc ctgcggccaa taaacaagga gaaaagtttc ggccggcaga
    13141 taaataccat acggagaact gctggcaggg acggtttcca aactgccggg atttttcatt
    13201 accacccttg cttctttgac aaattcgctt gcatcataga agcagacagt taaaatatct
    13261 actaaaatca accattggta aattttatac aaatgcctgt aaaactgctg caacaaccac
    13321 agccggctat tttttacgac gtggacggca ctcataaagc cgatacatgt gaaaacctca
    13381 aagccgccag caaacggggc gagctgcact tgggcgggtt ggctcatgga tgctatcccg
    13441 gcgtcaaaat gcccccaaaa atgctgcctg aattgtgtgt ggcctgtttc tgggatgccc
    13501 ataaagacca gagctggggg cttccaatgc accgcaacga ggggattgag ctgggttatc
    13561 taacccgcgg gcgtctggat ttcatcgtcg acggcgtaca ctacaagctc aaagggggag
    13621 acctgaccat tacccggcct tggcagccgc atcaggtcgg caatcctttg atttctgcca
    13681 gccgcatgca ttggctgatt ttagacgtca atgtgcggcg gcccaacgac ccatggaaat
    13741 ggccgccgtg gatcaatttt gcagcgcatg acctccagaa attaacccag ctattaagtc
    13801 ataacgaaca ggtcgtctgg cagggaaaca agcaaatcga aaaatgcttc gagcaaatcg
    13861 ccgagcatat acagaatgct gaaaagccgg aatctgttca aacccgactc cagttgtaca
    13921 ttaatgaact gcttcttgaa gtcttcgaaa tgctccagac ccgtaatatc aagcttaatg
    13981 cccgcctttc aagtacccat cgcagcgtcg agatgtttct ggcaggactg tccgaacatc
    14041 ttgaataccc ttggactctg gagtctatgg ctcgccattg caatctcgga cgcagccggt
    14101 tttcgcatta ctgcaggcag attaccaata tgaccgctgc tgattatctc attcattgcc
    14161 gaattgaacg tgcaaaaaaa atgctgtccg aatccaatgg aaagactatt ctggacatcg
    14221 ctatggcctg cggattcgaa tcaagccagt attttgctac ctgtttcaag aaaaaaaccg
    14281 gtacgactcc gactcaatac agaactgatg tgctgtccgg caaacagcaa agaaacgtcc
    14341 tataatcaaa aaaatacact ttcccattat atttaatttg cccattttaa aaatcgtttt
    14401 agttatacgc aactatcttt attacaaaag gttaaaactt tcttgggggg gactgtggaa
    14461 ttgttaaatc tggttttgat ggggattttt cagaaacaaa agtttgaatt gtgccgtttt
    14521 aaattataaa atcagttatc cgattgctat tatatagatt gttattatat aaggtattat
    14581 aatgtcttat aattcattcg gcagaaaaga gggatttact cttattgagt tgctggtggt
    14641 tattgccatc attgctctcc ttttatcaat aataattccc gctttaggca aggctaaaat
    14701 gcaagttgag gaaattctgt gccgaagcaa tcttcaccaa taccatattg ccacagaact
    14761 ttacaccaca gaagaggatg atacatatcc aaatccttgg gtttccctat ataaagaata
    14821 ccagtttccc gatgaaatac agcggtattg ccgatggcat aatcctcttt ataaccttga
    14881 ttcctatggt gaccggtacg gcggaccgta ttggtcttat ctgtccgcca caaaggccaa
    14941 tatctgccct acgtttcgaa aatatgccga acaattcggc caatttcata tgggcggcga
    15001 ccaagcaggt ctgacctgta ttggccagcc gtttgtctcg cagttcagct acagtatgaa
    15061 cgggattctg ggcgaccagc ggaatttcaa gggaattaag aaaagccaga tagctagttc
    15121 accttcgcag acatttctgt ggggagagga aaatatgtgg ccgctgtatg atgcagatgg
    15181 ctcgacaaaa ctgagcaggt atgtcctgaa tgacaccgcc ctgttgaccc ctggcgactg
    15241 cttcggcagt tttcacaagg tctcgaaagg ccagctttcc gcccagatgc cccaaacagc
    15301 aagaggatac ggaacctaca acgggggggt atccaatgta ctgatgatgg atggttcttc
    15361 ggcttcactg tcaccagctg gggggcaaac tctccagtat gccggccgca ttcgcagcgg
    15421 cggaacgcag taatccatcc cccctcaaaa aaagtgattc ctcttagggt tgccctgtta
    15481 ccagtccgcc taaaaagaat ccaaatccta ataccgtttc acaatgcggg caggaataca
    15541 ggacttcctt tttcacaacg ccctctgtct ggcggcgaat ttgattttgc tcaccttttt
    15601 taagcgttat cgttttttca caatacggac actttgccat aggaacttcc cgtatttttt
    15661 tcagtttcag ccccgaattc tcttccagag tttgacaatt ccccttgagc cggcaaacaa
    15721 ccatacgcca agagcaatct ggacaagtgg actgatcaaa gtgagtatcc ttgaaaatcc
    15781 gcctgaccag tagggcggat agttcccgtc aggcctgtga agtaataaaa aattatacaa
    15841 atggtgcata atcgaaggaa ttgctgccac aatgagatac agtccgatgc agcttacggc
    15901 aacgaccatt acatcctctt tgctgatagg atgggctatt tcaagcgatt tatcctcctt
    15961 aatcagccat tttgcgaatt tatcgctgag cacaataatc aagacagccc atcccagcgc
    16021 aaatgctagc aggagcagca ttcccataac ctgaagagcc agagaagcaa atcctgcatt
    16081 cgaattgtat cgtccggttg taaacagcac accgaatacc gtcggcacat agccgatagt
    16141 tttgagcagg ataaacacgc ccatcagctt aagcaccagc gacgcaattt cacgctttgt
    16201 cattgtttat ccttctattg gggatttggg ttcttcaggt cgatgttgcc gtgggtggtg
    16261 gtcagggtta ttgagccggt gccttcgccg atggttccgt tgagttgttc acccagaacg
    16321 ccctgcacca caatcgaccg gtccgtgcga atgtgtccgt acgacgttga ggcgctgaca
    16381 cgtccggcaa atccggcggg cggacaaaac gcgatattat tataggtggt actaagcgtc
    16441 gccgcaaggt tcgggctgga ttcattgacg catcgcagat caatcggagc atggcttgtc
    16501 cgggccgtga tccgttcaat ccccccaata tccccgccaa tggccccata gcttgtattc
    16561 aattcggcct cttttgccgt acatgccgtc agttcaatcg aatcctgtga cgtctgcaaa
    16621 tccagtcgat cggccaatat cgctttcaga cggatagggc cgtagatcgt cgaacagcga
    16681 acctgccgga tattggaatt atccaatttg attccgccgt gcgtggttgt ggcgttgacc
    16741 gaagcgtttt gtgtgttcgc gagtttaata tcgccatacg tcgaaaccgc gactatgtcg
    16801 ccgcggatgt tatcgaacag aatatccccg tgtgtcgact gcatggcgac ctgccgggtg
    16861 atattttctg cgcggcagtc gccgtatgtg gtcgtgattg ccgcccgggt ttccaacggc
    16921 acagtgattg tcatccggcc gctcacacaa tagttgtcct tatgttcctg cggttttcga
    16981 agcacaatca ccaaccgacc cggctcctga accaggtcca ctgcaagctg ctcagcgacg
    17041 gcatcggcga tctcccgcga gacagctgaa accgacaact ctgccagaac ggaacaatca
    17101 ttcgtaaccc aaccccacac tatataatga catgcgcagc aaacttttca caaaaagctc
    17161 cttaaatttt aatccttacg tttgttttca gatatctgga ttcatgctgc gccgagaacg
    17221 gatgttatcc caatcagtcc ggccataatc attcccacat aagccggcga ggcgatcgcc
    17281 agggcaatcg tacctgcatc cgaatttacc atacccgttt ggaaaggcaa aggttttcac
    17341 agacaaaaac acacaaacaa actcgcacac gccaatccca ataatgccca tttcatgcta
    17401 caatcctttc aaatcagttt tatagttttg agtgccctgc ttgttgagct tacttattca
    17461 gtttgatagc cagcggggcg accttatgtt ttctcacaat tttatctcct cttatccatc
    17521 gattattttg tgatacttat aaaattcata gaggacgatt attatcacaa aaaccataaa
    17581 cgctgaccag ttcagtacaa gagcgaaggg cagatattta tctttaaaaa attcttttcc
    17641 aagtgcaaat acaagtgaga ttgtcccggg aaaaagcaac agcacaaaaa agacaactat
    17701 tgcatgcgac tgcaaaaaag gactattttc aataagaatc gccgccgcta cggaaaagat
    17761 aaatataaaa ctcaggcaaa agtaccattg accattcgat aaaaccggaa tcggttttgg
    17821 ataggacggt tcaatttgtt gcgcgtggga gtcaataaaa tctataatgg cgggatgaag
    17881 tgtctggtct tctggcaacc agagagcgtg aatgcgctgc ctggttttaa agatgagctt
    17941 cttgagtccc ggaaattctt cgctggcttc aatccagtaa cccattaccc gtttccaata
    18001 aataaattta tccgcgtaat taaaccactt ttgagtgatt tgatgtgagc cacccagata
    18061 ccgaattaca taagggtata tgacaaacat ataaagcaat cctcctgctc caaacaaaaa
    18121 tatccctaaa aatattttta acaatcgtgc ctttatttct atctcatccg acaagacgtt
    18181 ttttagataa aggtacaggc catattcaat aataaaaatc aacggcagca aacgcggaat
    18241 aattacctta cccatattga aaagctgctg acaagaaaat tccacaaccg cccggggttt
    18301 tttccaaacg taagatgttt tattgttctt tgaagcgtcc atcatcgttt tgtaaaaaag
    18361 gaaaccgaga ggaatcgcct attcgcatgg agcttctatg cgccctgtat gaatcacttg
    18421 gaaagcatga cggtgttgct gtcggcatcg gtgaacaggc acgcaacagt tctctcataa
    18481 acaccctttt tgttaatgtc tcctttctga catctggatc aaagccgcac ccactgccga
    18541 agcggacccg attagtccgg ctccgattgc tccggcagaa taggttgagg cagccgctac
    18601 acgaataggg ctttcgtatt gattcggata gcccgttcta acttctactt tgctcgcaaa
    18661 ccatccaatc aaacaacaca tgagcaagct taacaatgcc agtacgaata gagcattttt
    18721 cattctgtta acctttcgaa tcatttttta catatttgga atccatatct tttttgcctg
    18781 aatcagttca acgtatgccg gcaaggtcag agccgtcgag gttttcgtat aaaaaacaag
    18841 ccctttgtaa gccgtcagat actgattctt tttgaaaaaa cccaccggag aaaacacaac
    18901 cagcgggaca cttgcggcct gcaaccgcag cagctgttca aatacatgcg gctctacctg
    18961 aacgattacc cccattgctt taatcgcatc ggtcatcgca gcgtacacga ctccaaccgc
    19021 agaaccggca gcgtctccgt ttatcatgat gggtctccta ttgaatattc atttacttgc
    19081 tcagcttgac ggtgttgccg tcggcgtcgg taaagctgcc ggccaccagc agaatcaaat
    19141 caatcagcca ccacagccca aggcctccgc aggtcagcag aaacaacacc cccgtgccag
    19201 ccttgcctac ataaaagcgg tggatgccca gcggacccag cagcagcaaa aacagcaggc
    19261 aagcgattcc tcttttttcc gatactccgt ttgccatagt caatctcctt tctgtctctg
    19321 acaagtcata ttttttcaac gccgcagccg agggcgtctt aatttattga cctttgaata
    19381 actaatggaa cgaatcatat tttaagtccg gcttttt
//
LOCUS       DAOTBO010000047        19413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2314_length_19413_cov_6.048145, whole genome shotgun sequence.
ACCESSION   DAOTBO010000047 DAOTBO010000000
VERSION     DAOTBO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 19413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19413
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2314_length_19413_cov_6.048145"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLE88_06940"
     CDS             complement(<1..185)
                     /locus_tag="PLE88_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76236.1"
                     /translation="MNDGRIVFSQIMDHFPRGDSSLAWTAIAAIIVSKPLHAWIGIIV
                     NPYPISIPKFLLYKVAAV"
     gene            complement(792..1376)
                     /locus_tag="PLE88_06945"
     CDS             complement(792..1376)
                     /locus_tag="PLE88_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76237.1"
                     /translation="MRVRKADEAGYAAALRGLAHNKKQEPQRMDIVARRLADMDGGHN
                     KFLESIIVWLDVRAPRYWWQEADTFRLSTKQSESTMHTLTEELAAIDIHNPAEVEAFI
                     ADNFEPNSCSPQTLAWIYETVPQKNIIEIKKKLPEGFLQTRMWCMSYKTLRNIILQRR
                     THRLPHWREFIRQVLEQVEHPELLPGLDTGKNGR"
     gene            1569..3083
                     /locus_tag="PLE88_06950"
     CDS             1569..3083
                     /locus_tag="PLE88_06950"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOM76238.1"
                     /translation="MMPNKKVFIRELKNYIGQQVRLDGWLYNSRSSGRVLFLMLRDGT
                     GLCQCVAEAANLSKELFVQLEHLGQESSLSVGGTIRAEPRSPGGLELAVSDAIIHCPA
                     ADYPITPKEHGIDFLLKNRHLHLRSLRPWCIGRIRHTVIDAIRRFFNDNGFILVDTPI
                     FTTIAGEGEQTLFEVDYFGQPMHLAQTGQLYLEAAAMSFGKVYCFGPTFRAEKSKTRR
                     HLTEFWMVEPEVAYIDLPGLLELAEDFVFSIIQGVLRNNRAELETLGADIAALEAIQK
                     PFVRLTYSEAAEILTSNRTKEFMAAQLEGFLAQKKAYEEELNQLLLLEAKGGLKKWQQ
                     DKTAGRIIELRGELAELQVKIENNPKHAQLAADFQWGKDLGGSDETIISLMHNRPVFV
                     THYPKKAKAFYMKTDRSREEVVENFDLLAPAGFGEIIGGSIREDNYERLLARIRQEGY
                     NLDSYQWYLDLRKYGSVPHGGFGLGVERTVAWLTGEKHIRQCIAFPRLMDKVYL"
     gene            3100..4248
                     /locus_tag="PLE88_06955"
     CDS             3100..4248
                     /locus_tag="PLE88_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOM76239.1"
                     /translation="MNFDKFLQDAGRWFVSSGLRIFIVLIITLVLVKAFRLLLDHAFS
                     RLEKCQNGKIKNHTETLKPLLYKIFVVVLAAAAVTLILENLGINVEGLLQTKIGSWLI
                     TSGLRIVLVLAVTYIIMKIIGLVLDHAFTRLEKRQDGEMKKRSDTLKSVVRSVINTAL
                     IAVAIIMVLDNLGLKVGPILTAAGVVGVAVGFGAQQLVRDIINGFFILLDDQIRVGDI
                     VDIAGKSGLVENVNLRMTTLRDFAGNVHYVRNGEITVVTNMTKEWSRYVFEIGVAYRE
                     NVDEVMEVIRQIDEELRSDAAFQGDILEPIEILGLDRFADSAIIIKARIRTRPSRQWA
                     VGREFNRRLKKRFDEKGIEIPFPHLKLYMGQGKDGTAPPLRVAMPPKV"
     gene            4352..5491
                     /locus_tag="PLE88_06960"
     CDS             4352..5491
                     /locus_tag="PLE88_06960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOM76240.1"
                     /translation="MKTYFLIAAVGLTAGWISAYGADSKTPMPAAPAAQNPAVQKEAV
                     VVTVNGEKVTEKEIADELAKRIEGQKKRMPAGMELPESYRQQMRKRVVDMKVEQILMN
                     QEAKKKNIAIPDAEVVAEITKIAGRQGQSMEDVEKEIANWGMTMEDLKGQIRYQMQIK
                     ALMKAENKNPEPSDEEVRKFYDDNPQHFDQPEQVRASHILIKVDSDAAAEEKAKAKEK
                     IEGILKRAKAGEDFAALAKEFSEDPGSKDTGGEYTFPRGQMVKPFEDAAFALEPNQIS
                     DIVETQFGYHIIKLSEKIAAGKTPFETVKNQIADYLANQKQNEYWNEYNKKIHESATI
                     EFSPEEKALRDQIEKEAAQRMLQQLQQQAQQQAQPQNKPEQKAEK"
     gene            complement(5616..5912)
                     /locus_tag="PLE88_06965"
     CDS             complement(5616..5912)
                     /locus_tag="PLE88_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76241.1"
                     /translation="MDTFPNSPEAVLPFVFIGCYMFVILLIAVLTVIIFCRIFAKAGY
                     HWALGLITLIPIGNIIMLLILAFSEWPIQRQLRTMQQAQATSQLGQLHPGVHSL"
     gene            6081..6785
                     /gene="msrA"
                     /locus_tag="PLE88_06970"
     CDS             6081..6785
                     /gene="msrA"
                     /locus_tag="PLE88_06970"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOM76242.1"
                     /translation="MRHIQQLIGIGLAGAAIAAGCDRAAPAGEYAASTYTKNTTEGAM
                     TDNQTADSDRKTGRSSGDNNYPIATFAAGCFWAVEYAFSQIPGVKATCAGFMGGTVDN
                     PSYRRVCRGDTNHAEVVQLRYNPQEVTYETLVKFFFLSHDPTTLNRQGPDTGTQYRSA
                     IFYHSREQKEIADKVKNRYTASGRFKKSIVTEITPAATFWKAEEYHQDYARKNPGSCH
                     TVDVKAILSEIAADSF"
     gene            6943..7524
                     /locus_tag="PLE88_06975"
     CDS             6943..7524
                     /locus_tag="PLE88_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76243.1"
                     /translation="MTVVVWLCTGGLAGAVLIEPYDYPNGTVLTASAEEVILSATASD
                     NQPVSIWPVTADIDPFGYAPTGQMVFSHAHICFWNTDRRLRMDFAQPVSTIQLTAGGG
                     SIFTSCQGRLEVYDIHFNLLDVYQTAPLTANQFETMLIFRPANDIYHAIAYSTGSNSF
                     GRLDHLEFAIPEPYSLLLLVGGAAIARWKRQWV"
     gene            7662..8867
                     /locus_tag="PLE88_06980"
     CDS             7662..8867
                     /locus_tag="PLE88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOM76244.1"
                     /translation="MAQRLISTVGLAVMVLLAWALSSDKRRLNWRLIGSGIALQLILA
                     ALLLETEGGQWFFEQARAVVTKIIQFSDTGAEFVFGANFKEHFFAFSVLPTIVFMSSM
                     MSVLFYLGVIQKIIQAMAKVMVYVMDVSGSESLANAANIFFGMAEAPLVIRPYLDTMT
                     RSELMAMMTGGMATISGATLAAYVGFGADAGHLLTASIISAPAGLVIAKIMVPETESS
                     LTKGTVRIDVPRKDSNVLDAACRGAAEGLQLALTVLAVLICFTAFVSLLNWVMSLLPE
                     LGGRPLTLERLLGWICAPLAWVMGVEWKDAAAVGGLIGKKTILNEFIAYRDLGAMKDT
                     LSRRSAVIATYALCGFANFGSVAIQIGGYSTLVPARRKDFASLGFKAMIGGSLAAFMT
                     AAIAGMLTA"
     gene            8971..9408
                     /locus_tag="PLE88_06985"
     CDS             8971..9408
                     /locus_tag="PLE88_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM76245.1"
                     /translation="METKSSSLPEQESACLSCGACCAYYRCSFYWAEADSVPGGTVPA
                     ALTEKLNDFRLAMRGMSGPNPRCIALLGEVGKSVRCSIYDLRPSVCREFPVAWENGQP
                     NPRCDKARMAWGLDPLNPPEAAPRPDDNPADQPPFRPPMPNAA"
     gene            complement(9520..11472)
                     /locus_tag="PLE88_06990"
     CDS             complement(9520..11472)
                     /locus_tag="PLE88_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOM76246.1"
                     /translation="MSILVFQDVYKSFGPEVVFDRLSVHFYAGQKVGLIGPNGSGKTT
                     LLRMILGTELPDAGKVICAKDLRIGYLPQEPVFDGQKTVMEEMYEEMSHILTLNYRIE
                     TFARQMESLSGDELKKAMAQYDKLCHQFETEGGYSCQARIKSILNGLDIGPELYNAKT
                     SVLSGGQLSRLGLARVLVKDTNLLLLDEPTNHLDLQATAWLESFLKTYSGSVILISHD
                     RYLLDKVAEKIVELENRRVRVWKGNYSQYVQTKETVILAERREHEKRLEMVTRTLDFI
                     ARNKDQEGMRKTARGRKIRLQRLLKENPDYLQTRKTVQSIQFSFAQTNIKSDLVLRAE
                     NISKSFGPLTLFENLSFDVVGGQALGITGPNGTGKTTLLKLALGQLAPDAGTIRLGAN
                     LKIGYLDQQANTLNPDHTVLEEMHSVRPDLTPQALRSRLGAFLFSGEDVFKKVGDLSG
                     GQQNRLMLCRLVLAQPDVLILDEPTNHLDIPSRQMLEEALADYNGAIIAVSHDRYFLD
                     RIADSLLVIGTDAAGRRQVGQTRLILSAAPDTDGVYSTYAQLIEQNRQAAQHQKIAQK
                     QKTAPPSGQPKTPTHLRPFNKYTIEQIEEMIEQQERTIEQMQNAFGDEKIYLNHQLLK
                     KLQADFDAEKQKLALLYEVWEYRAAD"
     gene            11694..11933
                     /locus_tag="PLE88_06995"
     CDS             11694..11933
                     /locus_tag="PLE88_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76247.1"
                     /translation="MKRQMTLVFTVAVAVCMGLMGCTTNNIGKTGLEAPGLTRQQVHQ
                     RHYEAIQNDLWQLQDDIDSFFLFDRPGRLSPMVVR"
     gene            12039..12896
                     /gene="cdaA"
                     /locus_tag="PLE88_07000"
     CDS             12039..12896
                     /gene="cdaA"
                     /locus_tag="PLE88_07000"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOM76248.1"
                     /translation="MKAILEYILNIGFYDRLIVAAELLLIGLFVYGIITFLKGTRGER
                     LFRGMIFVLVVGLLVLNLVVKMFGMDRLAYLYNSFLLVVLVVAVAAFQPELRRGLIRL
                     GQTRLFGSSPQQLTRSVEEIIKAVSQMAADRTGAIIVIPQQVALGEFIETGVRIDAKV
                     TSDLIQTIFYEGTPLHDMAMVIQGDRIVAARVQLPLAEASSRFGQLGSRHRAAIGVTS
                     GSDAIVIVVSEETGIVSLAMEGNLIRNISEAQLRRYLTTALVETTPVLEKLGKLTRTE
                     AQEDSSLNT"
     gene            12903..13943
                     /locus_tag="PLE88_07005"
     CDS             12903..13943
                     /locus_tag="PLE88_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76249.1"
                     /translation="MSDKVRTFLGVVFLTLLIWAWAYLSQEEKRSFTGTLEVSPATDP
                     GLLVTFTGKEGATQTRITLTSLNFKGAPSKISDLLKRYNLPQTDPDKERLNFYYNPTD
                     AGQTSGTYTLDILDYLRKSPKMHELALTLESCTPEQAEVTIEQLEEKKLAIQCLDENG
                     LPVKGAVADPAFVNIFVRKGYSGPATVTLSPLLKEAARSAPVTVTPYVSLGVAKIVRE
                     SKEPVKVSVQSEELLKTWSFQTTKPIGIIMSQALQNRYKVTIQNEIEVRTSTPIVATE
                     EAFRAYENVTYPLLIEILDRDAALPEIPPKRIIYNFPPEYVKRGEIALDETKIPRTAT
                     IKLEPINPLTVP"
     gene            complement(13921..15678)
                     /locus_tag="PLE88_07010"
     CDS             complement(13921..15678)
                     /locus_tag="PLE88_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOM76250.1"
                     /translation="MDALLLMLVCFVGYLVAYHTYGKFLARKIFCLRPEAPVPSRQHQ
                     DGIDFVPTRKGILFGHHFTSIAGTGPIVGPAIGIIWGWLPALLWVTLGCVLMGAVHDF
                     GTLVVSLRNDGKSISEIAARYINKRVRLIFFLIVFLALLIIIAIFGIVIATVFKLYPA
                     SILAVWLQIPIAMLLGWAIYRKNADVLFCTIAAVLAMYGCIALGAWLESVRPGMFNFP
                     ADWMIPPTGLWTILLLVYVWFASTLPVTMLLQPRDYINAWQLYIMMGLLACGIAASAF
                     LHPEFALTAPALQDVPSDAPPLLPMMFVIIACGAISGFHSLVASGTSTKQLQAEPDAL
                     FVGYGSMLLEGALAVLVIVCIAGGLSLGVSQMSGPQAWQHFYGLWMGEKGLESNLAPV
                     VIGASNVMTSLGIPAALGMAIIGVFISSFAGTTLDTATRIQRYVISELADDFHLPVLS
                     NRWIATTAAVLTAAALAFINTSEGHIVFGADAAGAKRLWPLFGTVNQLLAALALLVIT
                     MYLKRKGGLRYLVAAVPCLIMLLVTGWAMVLNEISYIKTKNVLLATVGGLVFLLAVWM
                     TAETVIQFSRSCSTGPSED"
     gene            complement(15738..17051)
                     /gene="der"
                     /locus_tag="PLE88_07015"
     CDS             complement(15738..17051)
                     /gene="der"
                     /locus_tag="PLE88_07015"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOM76251.1"
                     /translation="MALPVVAIIGRPNVGKSSLLNSLSGQMISIVDSMAGVTRDRVST
                     IINEGDRYFELVDTGGYGIVDTDALESHVENQIFQAIGSADVVLFMVDIREGVVPLDQ
                     TIAALLRKHSLQVILVANKADSPKQLALASEFLKLGFGEPICISAANLVNRAALMERI
                     CQMLDHLPCEKPAEAVMKLAVVGKRNAGKSTFINAVVGQERVIVSDVPGTTRDAVDVR
                     FEKDGRQFIIIDTAGVRKIGKMQNDIEYYGYTRVTRSIRRADVILFMMDAATKISQVD
                     KKLCALICQENKPCVIVVNKWDLVKNQAAAEDYADYLSKVLVGIRHAPIAFTTAKDGK
                     NIQSVLDLAAELFKQASAKIPTARLNKAVEMLASEKAAGSRKKAGIPKIYYAAQVASN
                     PVCILLFVNNPALFDQPYQRFIISRLGELLGLEEVPIRLLLRRRS"
     gene            17377..17931
                     /locus_tag="PLE88_07020"
     CDS             17377..17931
                     /locus_tag="PLE88_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOM76252.1"
                     /translation="MSNSNKNDSYVLFEASPSLWALVGTYVESAIFLAIAIILMLHPV
                     SKLAAYKIIPVVAVEKIQIGDTIAKYGGMGLAGIVVVNVLYQTIRLKRIRYEVNPDRI
                     EFTRGVFSRKIDNLDMFRVVDIKLHRSLLDCITGVGSVTLVTKDESDPVFEFEKVANP
                     KALYDVIKKASLDADRRQGVIHID"
     gene            complement(18196..18738)
                     /locus_tag="PLE88_07025"
     CDS             complement(18196..18738)
                     /locus_tag="PLE88_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76253.1"
                     /translation="MQIRVIKADGSTEPYLHTKVLGTLHRALAGAGRDGLDIAEMLAE
                     AITAFLYQQPSLGQLTAEQIHLMILSVLSGAGFEEAAEALQHHHLCRSLHRHRIEVVD
                     SQWLQWDKSRIVEDLVSRYRMDRLMARTIAGTVEEKILRLGLLRIRKTLIRHLVTADM
                     EALLEADRQLADVAQQYAAL"
     gene            complement(18771..19262)
                     /gene="nrdR"
                     /locus_tag="PLE88_07030"
     CDS             complement(18771..19262)
                     /gene="nrdR"
                     /locus_tag="PLE88_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOM76254.1"
                     /translation="MRCPFCKEDNDKVIDSRSSEGGRIIRRRRECLLCGRRFTTYEKI
                     GEAFKLSVIKKDGTRVPYDRDKLVAGLQKACYKRPVSAEKIQDIADRIEEEVFRNYDK
                     EVSSLFLGEQAMRFLRKVDKVAYIRFASVYRDFTDAGALLDEVSQAIREPDNADQPHL
                     FNV"
BASE COUNT         5020 a         4479 c         5088 g         4826 t
ORIGIN      
        1 acagccgcca ccttatacaa cagaaatttt ggtattgata tcgggtaggg gtttacaata
       61 ataccgatcc aggcatgtaa aggttttgat acgatgatcg ccgcgatagc ggtccacgca
      121 agacttgaat cgcctcgcgg aaagtggtcc atgatctgtg agaatacaat tcgaccgtcg
      181 ttcatgattg gctccttgaa gtaatccaga gcgattatca tacacgagca aattgaaatc
      241 gatacaggtc ataattttct ttaagtcttt aaattgtttc gtgttataat aaaaatgtta
      301 aaagttaacg ggacagtact gatgtcgaat ttaatcttga aaattttcta agtcgggcat
      361 atagtcaacc tgcgatgtct tttatttgcc gagcactcgt gaatataact tcttttttta
      421 tcagaggtta gcttacttag actggggtga gagagaattt aaccttgtaa aaaactctcc
      481 gccgtcgaaa atttattcgt ccgataaaat attaaaattt tgttggacaa aatattttaa
      541 atttgttaaa gagaaaaaaa cagcttagcc gatttaaaag atacaagagt tttctcccct
      601 ccggaaaaca tcagggaata aggtttcctg atgttttctt tttttaatta gtcagcgtca
      661 gcagtcagct tttatcaata ttcaatcagc cagccccaat aaaaagattt cttttcaaaa
      721 caagcaaaat agatttataa atgcctatcg aactgttaaa cagctgccgg cttcgaaaga
      781 tattctaaag ttcagcgtcc atttttgccg gtatcaagac ccggcagcag ttcggggtgt
      841 tcaacctgct cgagcacctg acggataaat tcgcgccaat gcggcaggcg gtgcgtgcgg
      901 cgctgtaaaa tgatgttacg gagggttttg tagctcatgc accacattcg ggtctggaga
      961 aaaccctcgg gcagtttttt ctttatttca atgatatttt tttggggtac cgtttcatat
     1021 atccaagcaa gcgtttgggg actgcatgaa ttgggctcga aattgtcggc aataaatgcc
     1081 tcaacttctg ctggattatg gatatcaata gcggccagtt cctcggtaag cgtgtgcatt
     1141 gtgctttcac tctgcttggt agaaagccgg aatgtatcgg cttcctgcca ccaatagcgc
     1201 ggagcccgga catccagcca gacaataatg ctttccaaaa acttattgtg gccgccatcc
     1261 atatctgcca gccggcgggc tacaatgtcc attcgctggg gttcctgctt tttgttgtgt
     1321 gccaaacccc gcagtgcagc cgcatagccg gcctcatctg cttttcgtac tcgcatataa
     1381 aaggattcca tccctgttgt ctccgcattt tttgccgcat agagtaatcc ggcacagatt
     1441 gtccgtcaat cctattttat ccgctggatc ggtgaaaaca ctggattaac tatcttgcag
     1501 aaagcaatga tttcggatac agtttaagcc tgaattggat tcaaatcaca gcaaaggaac
     1561 tattgccaat gatgccaaat aagaaggttt ttattcgtga actgaaaaat tatattggtc
     1621 agcaggtacg gctggacggc tggctgtata acagccgctc gagcggcagg gtgctgtttt
     1681 tgatgcttcg cgacggcaca ggtctatgcc aatgcgtcgc agaggcggcc aatctttcaa
     1741 aagagctgtt cgtgcagctt gaacatctgg gtcaggaatc atcgctgtct gttgggggca
     1801 ctatccgggc agagccgcgt tcgccgggcg ggttggagct ggccgtcagc gatgcgatta
     1861 ttcattgtcc ggcagcggat taccccatta caccgaagga gcatgggatt gattttctgc
     1921 tgaaaaaccg tcatctgcac ctgcgttctc tgcgtccgtg gtgcattgga cggatacggc
     1981 acacggttat cgatgccatt cgccggtttt tcaacgacaa cggctttata ctggtagata
     2041 cacccatttt tacgacgatt gccggcgaag gagaacagac tttgttcgag gttgattatt
     2101 tcggccagcc catgcatctg gcgcagacag gccagctgta ccttgaagcg gcggcgatga
     2161 gctttgggaa ggtgtactgt tttggcccga cctttcgggc ggaaaaaagc aaaacccgcc
     2221 ggcatctaac ggaattctgg atggtagagc cggaagtagc ttatattgac ctgccggggc
     2281 ttctggaatt ggccgaagat tttgtcttca gcatcattca gggggtactt cgaaacaatc
     2341 gtgcggaact ggaaactctc ggtgccgata ttgcggcatt ggaagctatt caaaaaccat
     2401 ttgtgcggct gacctatagc gaagcagccg agattctgac aagcaaccgc acgaaagagt
     2461 ttatggcagc tcagcttgaa ggatttctgg cacagaaaaa ggcatacgaa gaggaattaa
     2521 accagttatt attattggaa gccaaagggg gccttaaaaa atggcagcag gacaaaacgg
     2581 ccgggcggat tatcgaactt cgcggggaat tggcagaatt gcaggtaaaa attgaaaata
     2641 atcctaagca tgcacaatta gccgctgatt tccaatgggg caaggatctg ggtgggtcgg
     2701 atgaaactat tatttcgctg atgcacaatc ggcctgtatt cgtaactcat tatcccaaaa
     2761 aggctaaggc gttttatatg aagacggacc gcagcaggga agaggttgta gagaattttg
     2821 accttttagc tccggccggc tttggcgaga taattggggg ttcgattcgg gaggacaatt
     2881 acgaaagatt gcttgcccgc attcgccaag aagggtacaa tttagattct tatcaatggt
     2941 atctggattt gcgaaagtac ggttcggtac cgcatggcgg ctttggtctg ggcgtagagc
     3001 gcactgttgc ctggcttacg ggcgaaaaac acatccgcca gtgtattgcg tttccgagat
     3061 tgatggacaa ggtttatttg taaatagaaa attgaatcta tgaattttga taagtttttg
     3121 caggatgccg gccgctggtt cgtcagcagc ggcctgcgga tttttatcgt tctgattata
     3181 acgctggtgc tagttaaagc gtttcgcctg ctgctggatc atgcattttc acgtctggaa
     3241 aaatgccaga acggcaaaat taaaaatcac acagaaacac tgaaaccgct gctgtataaa
     3301 atctttgtcg ttgtcttggc tgcggcggct gttacgctga tactggaaaa tctgggaatc
     3361 aatgtagaag ggcttttgca aacaaaaatc ggcagctggc tgattaccag cggcctgcgt
     3421 attgtcctgg tgctagccgt aacttatatc attatgaaaa tcatcgggct ggtgctggat
     3481 catgcgttta cccgtcttga aaaacgtcag gacggcgaaa tgaaaaaacg ctcggatacg
     3541 ctgaagtctg ttgtgcgcag cgtcatcaat acggctttga ttgctgtagc cattattatg
     3601 gttctggata atctggggct gaaagttggt cctattctga cagccgccgg cgttgtaggt
     3661 gtggcggtgg gctttggcgc ccagcaactc gtgcgggata taatcaacgg cttttttatt
     3721 ctgctggatg accagatacg ggtgggtgat attgtggata ttgccggcaa aagcgggctg
     3781 gtggaaaatg tcaatctgcg gatgacaacg ctgcgggact ttgcgggcaa tgtgcattat
     3841 gttcgcaacg gtgaaattac tgtcgttacc aatatgacca aagagtggtc gcggtatgtg
     3901 ttcgaaatcg gcgtggccta tcgtgaaaat gtggatgaag taatggaggt gatacggcag
     3961 attgatgaag aactgcggtc tgatgcagca tttcaaggag atattcttga gccaatagaa
     4021 atcctcgggc tggaccgctt tgcggattcg gcgatcatta ttaaggcccg cattcgaacg
     4081 cggccgtctc gacagtgggc ggtcggacgg gagtttaacc ggcggctcaa gaagagattt
     4141 gacgaaaaag gcatcgaaat tccgttcccg cacctgaagc tgtatatggg acagggcaaa
     4201 gacggaacgg cgccgccgct gcgggtagca atgccgccga aagtgtaaaa ctgcggcact
     4261 gccggccagc ggcagaaaaa attatacgct gtgacgggcg aaagtaaggc ccgtccttat
     4321 tttttggcag aactcaaaag aaagggaaaa aatgaaaacg tattttttaa ttgcggctgt
     4381 cggattgaca gctggctgga tcagcgccta tggtgccgac agcaaaacac ctatgccggc
     4441 agcgcctgcc gctcaaaatc ctgccgttca gaaagaagcc gttgtcgtta cagtcaacgg
     4501 tgaaaaagtg acggagaagg aaattgccga tgaactggcc aagcgtatcg aagggcagaa
     4561 aaagcggatg cccgcaggga tggaactgcc tgaatcctac cgtcagcaga tgcgcaagcg
     4621 ggtggtggat atgaaggtag agcagatcct gatgaaccag gaagccaaga agaagaatat
     4681 cgctattccc gatgctgaag tcgtggcgga aatcaccaaa atcgccggac ggcagggtca
     4741 gtcgatggaa gatgtggaaa aagagattgc caactggggt atgacgatgg aggacctgaa
     4801 agggcagatt cgctatcaga tgcagattaa agctctgatg aaggcggaaa ataaaaatcc
     4861 ggaacccagc gacgaagagg ttcgaaagtt ctatgatgac aatccgcagc actttgacca
     4921 gcccgagcag gttcgcgcca gtcatattct gattaaggtt gattcggacg ctgcggcaga
     4981 agagaaggcc aaggccaagg agaaaattga gggtattttg aagcgggcca aagccggaga
     5041 ggattttgct gcactggcca aggaattcag cgaagatccc gggtccaagg ataccggcgg
     5101 ggaatatacc ttcccgcgtg ggcagatggt caagccgttt gaagatgcgg cttttgcact
     5161 ggagcccaat cagataagcg atatcgtcga gacccaattc ggctatcaca tcatcaagct
     5221 gtctgaaaag attgcagccg gtaaaacgcc ctttgagacc gttaaaaacc agattgccga
     5281 ctatttggcc aatcagaagc agaacgaata ttggaatgaa tataacaaaa agattcacga
     5341 aagcgccact atcgaatttt ctcctgaaga aaaagccctg cgggaccaga tagaaaaaga
     5401 ggcggctcag cgaatgctgc agcagcttca gcaacaagct cagcagcaag cacagccgca
     5461 gaataagcct gaacaaaagg cagaaaagta gtccatagaa tagcccacaa accgccggcc
     5521 tggtctgaca ggtccggcgg ttttttgttt gtccgaaggg attttactaa tgaccttctc
     5581 gtccagctga aatgccagtc tgtttttgac agacgttaga gactgtgaac accgggatgc
     5641 agctgtccga gctgcgaagt cgcctgtgcc tgctgcattg tgcgaagctg acgctgtata
     5701 ggccattcgc tgaatgccaa tatgagcagc ataatgatat tgccgatagg aatcagcgta
     5761 atcagtccca gcgcccagtg atagccggct ttggcaaaga tgcggcagaa gatgattact
     5821 gtcaaaaccg caatcagcag gatgacaaac atataacagc cgatgaatac aaacggcaga
     5881 acggcctcag gagaattggg gaaagtatcc atatctttcc tttcggttta agatgttttt
     5941 tttttattct aacagcaaaa cgagaatcag gcaaatataa tgcaaattag ctatcctgaa
     6001 cggatattgg aaggggcagc gaaccggccg gattttagct cgtattgaat taataaacag
     6061 ctatcataaa aggggtatcg atgcggcata tacagcagct aatcggaata gggctggcag
     6121 gagcagcaat agcagccggc tgtgacaggg ccgctccagc aggtgaatat gccgcatcaa
     6181 cctatacaaa gaatacaaca gagggtgcta tgacagacaa ccaaacagca gacagcgata
     6241 gaaaaaccgg ccgatcttcg ggagacaaca actatccaat agcaaccttt gcagcaggat
     6301 gcttttgggc agttgagtat gcattcagtc aaattcccgg agttaaggca acctgcgcag
     6361 gctttatggg gggcacagtt gacaatccca gctaccgccg cgtatgcagg ggcgatacta
     6421 atcatgccga agtagtgcaa ctgcgctaca atccccaaga ggtaacctat gaaacgctgg
     6481 taaaattttt ctttctgtcg cacgacccga caacactgaa tcgtcaaggg ccggacacag
     6541 gtacgcagta tcgctcagca attttctacc acagccggga acaaaaagaa atcgccgata
     6601 aggtcaaaaa ccggtacaca gccagcggcc ggttcaagaa atccattgtt acagaaatta
     6661 ccccggcggc aaccttttgg aaagccgaag agtatcatca ggattatgcc cgcaagaatc
     6721 ccggctcctg tcataccgtc gatgttaagg ccattctgtc tgaaatagcc gccgactcat
     6781 tttaagctca ccctctatcc gctggagggc tgcagcggca agttttgtca gtgaaatcac
     6841 cggctgcaat tgacaatccc cccttgcatt tgctataata agccgctgga gaaaagagga
     6901 tcattttgtg gaggaaggaa agatgaaaaa aaaacggttc tgttgacagt tgttgtctgg
     6961 ttatgcacgg ggggattagc gggggcagtt cttattgagc cgtatgatta tcctaacggc
     7021 accgtcttaa cagcaagcgc ggaagaggtg atactttcag ctaccgcttc cgacaaccag
     7081 cctgtgagca tctggcctgt tacagcagat attgacccgt ttgggtatgc accaacgggg
     7141 caaatggttt tcagtcatgc acatatctgc ttctggaata ccgatcggcg tctgcgaatg
     7201 gattttgccc agccggtttc aacaatacag cttactgctg ggggaggcag tatttttaca
     7261 tcctgccaag ggcggctgga agtctatgat attcatttta atttactgga tgtgtatcag
     7321 acagcacctc tgacagccaa tcagttcgag accatgttga ttttccggcc ggctaatgat
     7381 atttatcatg caattgctta ttccaccggg tctaattcgt tcggccggct tgaccatttg
     7441 gaatttgcca ttcctgaacc gtacagtctg ctgctgcttg tggggggagc tgccattgca
     7501 cgatggaaac ggcaatgggt ttaaagaggc cgtagatttc aactgagctt aaaaaaatct
     7561 ccggctccaa agcgagatga cataatagga ctttagattt ggtctggtca attcaaaaga
     7621 gtgcggtata atagacctct cggaaataca caggaggtct tatggctcag cggcttatca
     7681 gtacagtcgg attagcggtt atggttctct tggcctgggc gctgtcgtcg gacaagaggc
     7741 gactgaattg gcggctgata ggcagcggca tagcacttca gctgattctg gcggcgctgt
     7801 tgctggaaac ggaaggcggg cagtggttct tcgagcaggc ccgggcagtc gttaccaaaa
     7861 tcattcaatt ttccgatacg ggtgccgagt ttgtcttcgg ggcaaatttc aaggagcatt
     7921 tctttgcctt ttctgtattg ccgacgattg tctttatgtc gtcgatgatg agcgtcttgt
     7981 tttacttggg tgtgattcag aagattattc aggcaatggc caaagtaatg gtctatgtga
     8041 tggatgtgtc gggttcagaa tccttggcca atgctgctaa catttttttc ggaatggccg
     8101 aggctccgct ggttatccga ccatatctgg acacaatgac ccgctcggag ctgatggcga
     8161 tgatgaccgg aggaatggcc acgatttccg gggctacttt ggcggcgtat gtcggttttg
     8221 gggcggatgc ggggcatttg ctgacggcct cgattatctc ggctccggcc ggactggtca
     8281 tcgccaaaat aatggttcct gaaaccgaaa gttcgctgac caaaggaacc gtgcgcattg
     8341 acgtaccccg caaagattcc aatgtattgg atgccgcctg ccgcggggct gcggagggat
     8401 tgcaacttgc cctgaccgtg ctggcagttc ttatctgctt tacagcattt gtatccttgc
     8461 ttaactgggt aatgtcgctg ctgccggaat tggggggcag acctctgacc ttagagcgtc
     8521 ttctgggctg gatttgtgcg ccgctggcgt gggtgatggg tgtggaatgg aaggatgccg
     8581 ctgctgtggg aggattgatt ggaaaaaaga ctatccttaa cgagtttatc gcctaccgcg
     8641 atttgggagc aatgaaggac acactcagca gacgttcggc agtcattgca acctatgcgc
     8701 tgtgcggttt cgccaatttc ggctcggtgg ctattcaaat cggcgggtac agcactttag
     8761 tccctgcccg ccggaaggat tttgcttcat tgggctttaa ggcgatgatt ggcggctcac
     8821 tggcggcttt tatgactgca gcaatcgctg gtatgctaac ggcttgacat aacctcaagc
     8881 agaaggtaga attcgggcaa acggcaaaaa gaacatctgt taaaaagtaa tgattttgac
     8941 cggccgctgc acgtctgcgg ctattttagt atggaaacaa aaagcagttc tttaccagaa
     9001 caagaatcag cctgcctcag ctgcggggcc tgctgcgctt attaccgatg ctcattttac
     9061 tgggcggagg cagacagcgt ccccggagga actgttccgg cggcactgac tgagaagctg
     9121 aacgattttc gtttggcaat gcgtggaatg agcggaccca atccccgctg catcgccctt
     9181 ctgggtgaag tcggtaaaag tgtccgatgc agcatctatg acctgcggcc gtcggtttgc
     9241 cgggaatttc cggtggcatg ggaaaacggc cagcccaatc cgcgatgcga taaggcacgt
     9301 atggcatggg gacttgaccc gctgaatccg ccggaagctg cgccccggcc agacgacaat
     9361 ccagctgatc agccgccctt tcggccgccg atgcctaatg ctgcataagt ctttaagatt
     9421 atgcttaatt ctctatcttt tgtatcggaa acgaggcatt ttgatagatt tcactctttt
     9481 agagaaatga tcccgggagg aaatcagaga aaaagcattt tagtcggcgg cacgatactc
     9541 ccagacttca tagaggagag ccagcttctg tttttcggcg tcgaagtctg cttgaagctt
     9601 ttttaaaagc tgatggttca gatagatttt ttcatccccg aaggcattct gcatctgctc
     9661 aatcgtgcgt tcctgctgtt caatcatttc ctctatctgt tcgatggtgt atttgttaaa
     9721 aggccgcagg tgggtcgggg ttttgggctg tccggagggg ggggcggttt tctgtttttg
     9781 agcaattttc tggtgctgtg cggcctgccg gttctgctcg ataagctggg cataggtaga
     9841 gtacacgcca tcggtgtctg gggcagcaga aagaatcagg cgtgtttggc cgacttgtct
     9901 tcggccggcg gcatccgtac cgataaccag caacgaatca gcgatgcggt cgagaaaata
     9961 gcgatcatga ctgactgcga taatcgcccc attgtagtca gccagtgctt cttcgagcat
    10021 ctgcctgctg gggatatcca aatggttggt cggctcatcg agaatcagca catccggctg
    10081 ggccaagacg agacggcaga gcatcaggcg gttctgctga ccgccagaca ggtcaccgac
    10141 ttttttaaag acatcctcgc cggaaaacag aaatgctccc agccgactgc gcagcgcctg
    10201 cggcgttaaa tccggccgga cagaatgcat ttcttccaga actgtatggt ctggattgag
    10261 agtatttgcc tgctggtcaa ggtagccaat cttaagattg gcacccaaac gaatcgtgcc
    10321 ggcatcaggg gcaagctggc ccagagccag cttgagcaga gttgttttac ccgtcccgtt
    10381 ggggccggta attcccaagg cctgtccgcc gacgacatca aatgaaagat tttcaaacag
    10441 ggtcagcggc ccgaaggatt tgctgatatt ttcagcacga aggaccaaat cgctttttat
    10501 atttgtttga gcaaagctga attggattga ttgtacagtc ttgcgggtct gcaagtagtc
    10561 cggattttct ttgagcagac gctggagacg gattttacgg ccgcgtgcgg ttttgcgcat
    10621 tccttcctga tctttgttgc gggcgataaa atccagtgtg cgggtgacca tttcgagtcg
    10681 tttttcgtgc tccctccgtt cggccagaat gacggtttct ttggtttgaa catactggct
    10741 gtaattacct ttccagacgc ggacacggcg gttttccagt tcaacaattt tttcggctac
    10801 tttatccagc aggtagcggt catggctgat caggatgact gaaccgctgt aggtctttaa
    10861 aaaagattcc agccacgctg ttgcctgtaa atccagatgg ttggtcggtt cgtcgagaag
    10921 caggaggttg gtgtctttaa ccagcactct ggcaagcccc aagcgggaca gctgaccgcc
    10981 gctgaggaca gaagtttttg cgttatagag ctctggacca atatccaatc cgtttaaaat
    11041 ggatttgatg cgtgcctgac aggagtaacc gccttccgtt tcgaattgat gacagagttt
    11101 atcgtattgg gccattgctt tcttcaattc gtctcccgag agactttcca tctggcgggc
    11161 aaaggtctct atgcggtaat tgagggtcag aatatgcgac atttcttcat acatttcttc
    11221 cataacagtc ttttgaccat caaagaccgg ctcctggggc agatagccaa ttcgcaagtc
    11281 tttggcgcag atgaccttgc cggcatcggg cagttcagtg ccgagaatca ttcgcaagag
    11341 agttgttttg ccagacccgt taggaccaat caaaccgact ttttgtccag catagaaatg
    11401 cacagacaaa cggtcaaaaa ctacctctgg gccgaaggac ttgtaaacat cctgaaatac
    11461 gagtatagac ataactattc ggatagcatt ttttaggggc tgattgtatc catttttatg
    11521 ctgttgttca aataaaacac gaaatcctta atttaacgag gcttaaaacg attttttttt
    11581 gacttattaa aaaaatagca ttacaatcga taaaggtggc taatgttgtg cgcggacatt
    11641 ttaaacgctg cgagcaggac tgatttaaga ttatatttca taagggttta gcgatgaaaa
    11701 gacagatgac attggtgttc actgttgcgg ttgcagtatg tatgggactt atgggatgta
    11761 cgactaataa cattggcaaa acagggctgg aggccccggg tctgacccgc cagcaggttc
    11821 atcagcgtca ctacgaggcc attcagaatg atctgtggca gcttcaggat gatatagatt
    11881 cgtttttcct gtttgaccgg ccggggcggt taagtccgat ggtggttcga taaactgctt
    11941 tggggcggcg gcattgattg ctatcgtata gaaagatatg aagcacaggt cgatttaatg
    12001 ttaatgcccg aatcggtttc gtgcaaggaa gctgattttt gaaggcgatt cttgagtata
    12061 ttctgaacat cggtttttat gatcggctta tcgtagcagc ggaactgctg ctgataggtc
    12121 tgtttgttta cggcatcatt actttcctca aagggacccg cggtgaacgt ctttttcggg
    12181 gaatgatatt tgttctggta gtcgggctgc tggttctgaa cctggttgtt aaaatgtttg
    12241 ggatggaccg gctggcttat ctgtataaca gttttctgct ggtggttctt gtagtagcgg
    12301 ttgcagcgtt tcagccggag cttcggcggg gtctgatccg gctgggccag acgcggcttt
    12361 tcggcagttc gccgcagcag ctgactcgtt cggtagagga aattatcaaa gcagtatccc
    12421 aaatggccgc tgaccgcacg ggtgcgatta tagttattcc gcaacaggtt gctttggggg
    12481 agtttatcga aaccggtgta cggattgacg ccaaagttac cagcgatctg attcaaacca
    12541 tattttacga aggcacgccg ctgcatgata tggcgatggt aattcaggga gaccgtattg
    12601 ttgcagctcg ggtgcagctg ccgctggcag aagccagcag ccgctttggt cagctgggat
    12661 cgcggcatcg ggcagccatc ggcgtcacca gcgggtcgga tgcaattgtt attgtggtca
    12721 gcgaggaaac aggaattgtt tctctggcga tggaaggcaa tctgattcgc aatatatcgg
    12781 aggcacagct tcgccggtat ctaacaacag cactggtgga gacgacgcct gttctggaaa
    12841 aactgggcaa actgacccga actgaagcac aagaagacag ttcgttaaat acgtaaatcc
    12901 ctatgagcga taaagtgcgt acatttttag gagtggtttt cttaacgctg ctgatttggg
    12961 catgggctta tctgtcacag gaagaaaaac ggtcgtttac aggaactctg gaggtttctc
    13021 cggcaactga ccccgggctg ctggtaacat ttacaggcaa ggagggagcc acgcagacgc
    13081 ggattacgct gacttcgctg aatttcaaag gggcaccatc caagatttct gatcttttga
    13141 agcgctacaa tctgccgcag acagatccgg acaaggagcg cctgaacttt tattacaatc
    13201 cgacagatgc cggtcagacg tcgggaacct atacactgga cattctcgat tatcttcgca
    13261 aaagccctaa gatgcatgag ctggcgctga cattggaatc gtgcacacca gagcaggcgg
    13321 aggttactat tgagcagctg gaggagaaaa aactggcgat tcaatgtctg gatgaaaatg
    13381 ggcttcctgt taaaggtgct gtagcagatc ctgcttttgt aaatatcttc gttcgaaaag
    13441 ggtacagcgg ccctgctaca gtaacgcttt cgccgctgct gaaagaagca gcccgcagcg
    13501 cgccggttac cgtaacaccc tatgtatcgc tgggagtggc caagattgtc cgcgaatcaa
    13561 aagagccggt gaaagtcagc gttcaaagcg aagagctttt aaagacttgg tcgtttcaaa
    13621 ccaccaaacc catcggcatt attatgagcc aggcgcttca gaatcggtat aaggtaacga
    13681 ttcagaatga aattgaagtg cgcacttcga cgccgatagt ggctaccgag gaggcgttcc
    13741 gggcttatga aaacgttacc tatccgctgc tgatagaaat tcttgaccgc gatgccgccc
    13801 ttcccgagat accccccaag cggattatct acaatttccc gccggaatat gtcaagcggg
    13861 gagaaattgc gctggatgaa acaaaaattc cgcgcactgc aactattaaa ctggagccga
    13921 ttaatcctct gacggtcccg tagagcagct tcgggaaaac tggatgacag tttcagctgt
    13981 catccacaca gccaggagaa aaacaagccc tccgactgtt gccagcagga catttttcgt
    14041 tttgatataa ctgatttcat tgaggaccat cgcccagccg gttacaagga gcattatcag
    14101 acagggcacg gcggctacca gatagcgtaa accgcctttg cgtttgaggt acattgttat
    14161 caccagaagc gccagcgctg ccagaagctg attaacggtt ccaaagagcg gccagagccg
    14221 tttagcaccg gcggcatcag caccgaagac aatgtgccct tcggatgtat tgatgaatgc
    14281 cagcgctgcg gcggtcagga cggctgccgt cgtggcaatc caacggttgg acagcacagg
    14341 caggtgaaaa tcatcggcca gttcactgat aacatagcgc tgaatacgcg tggccgtgtc
    14401 aagggtagtt ccagcaaaag aggaaataaa caccccaata atagccattc ccagcgccgc
    14461 aggaatgccc agcgaagtca ttacattcga agcccctatg accaccggcg ccagattgct
    14521 ttcgaggcct ttttcgccca tccagagacc atagaagtgc tgccaagcct gcgggccgga
    14581 catttggctg acacccagac tcaagcctcc tgcaatacaa acaatcacca acactgccag
    14641 tgcgccttcc aaaagcattg aaccgtagcc gacgaacaag gcatctggct cggcttgaag
    14701 ctgcttggtg ctggttccgc tggccaccag tgaatggaag ccgctgatgg caccgcaggc
    14761 aatgatgaca aacatcatcg gcagcagcgg cggagcatct gagggaacat cctgcaaggc
    14821 cggagcagtc agtgcgaatt ccgggtgcag aaaggccgaa gcggcgatgc cgcaggccaa
    14881 aagccccatc ataatataaa gctgccaagc gttaatataa tcgcgcggct ggagcagcat
    14941 tgttaccggc agggtcgagg caaaccagac ataaaccagc agcagaattg tccagaggcc
    15001 tgtcggggga atcatccaat cggctgggaa attgaacatc cccggccgga ccgattccaa
    15061 ccaggccccc agcgcgatgc agccgtacat tgccagtaca gctgcaatgg tacagaacaa
    15121 gacatcggcg tttttcctgt aaattgccca gcccagcagc attgcgatgg gaatctgaag
    15181 ccagacggcc aagatagaag cagggtagag cttgaaaacc gttgctatca cgataccgaa
    15241 gatggcaata ataatcagca gtgccagaaa gactatgagg aaaaaaatca gccggacacg
    15301 cttattgata tagcgggcgg ctatttccga tatgcttttg ccgtcgtttc gcagggaaac
    15361 gaccagcgtt ccgaaatcgt gtacagcacc catcagcacg caaccgagtg ttacccacaa
    15421 gagagccggc agccagcccc agataatgcc gatggccgga ccgacaatgg gaccggtacc
    15481 agcaatggaa gtaaaatgat gaccgaataa gatccctttt cgggttggga caaaatcgat
    15541 gccatcctga tgctggcggc tgggcacagg ggcttcagga cgcagacaga aaatcttgcg
    15601 ggctagaaat ttgccgtacg tgtgataagc taccaaatat ccgacaaaac aaaccagcat
    15661 tagtaaaagt gcatccatag caaacctcct gtttggcagt accgtagagc atcacgctca
    15721 aaactgacct tcatttatta gctgcgtctg cgcagcagca aacgtatcgg gacttcttcc
    15781 aaacccagca gttctcccag ccggctgatg ataaaccgct gatagggctg gtcaaacagt
    15841 gccggattat taacaaacag cagaatgcag acgggattgg aggccacctg tgcggcataa
    15901 taaatcttcg ggatacccgc ctttttgcgg gagccggcgg ccttttccga agccagcatc
    15961 tcaacagctt tgtttaatcg ggcagttgga atctttgctg aagcctgctt aaaaagttca
    16021 gccgccaaat cgaggacgct ttggatattt tttccgtcct tggcggtagt aaaggcaatg
    16081 ggggcatggc gaatgccgac cagaaccttg ctgaggtagt cggcataatc ttcagcagca
    16141 gcttggttct tgaccaagtc ccatttgttg acaacaatca cacagggttt gttttcctga
    16201 caaatcaggg cacacagttt tttatcgacc tgcgaaatct ttgttgcggc atccatcata
    16261 aagaggataa catcggcccg acggatgctt cgggtaaccc gtgtatagcc gtaatattcg
    16321 atgtcgttct gcatttttcc gatttttctt accccagctg tatcaataat aataaactgg
    16381 cgcccgtcct tttcaaaacg gacatccacc gcgtcacggg tagtgccggg aacatcactg
    16441 acgatgacac gttcctgccc gacgacggca ttgataaagg tgcttttgcc ggcattgcgc
    16501 ttgccgacta cagccaattt cattactgct tcagcaggct tttcgcaggg cagatggtca
    16561 agcatttggc agattcgctc catcagggca gcacggttga caaggttggc agctgaaatg
    16621 cagattggct cgccgaaacc cagtttgagg aattcgctgg ccagtgcaag ctgctttggg
    16681 ctgtcggcct tattggcaac cagaattact tggagcgagt gcttgcgaag gagagcagcg
    16741 attgtttggt caaggggaac caccccctct cggatatcaa ccataaacag cacgacatcg
    16801 gcagaaccga tggcctgaaa aatctgattt tcgacgtgtg attcaagggc atcggtgtct
    16861 acaattccat agcccccggt atccaccagc tcaaaatacc ggtcgccttc gtttatgata
    16921 gtgctgacgc ggtcgcgcgt tacgccggcc atcgagtcca caatgcttat catctgcccg
    16981 gacagcgaat tgagcagact gcttttgcct acattgggac gcccgattat tgctacaaca
    17041 ggtaatgcca ttgggtattt ccgttctcaa atattcaatt tcagcgaaat tataaccgaa
    17101 ataacatttt atgggaagta gcaaccgctt tgtataattt aacttgcaaa attaaccggt
    17161 gcgaatacgc tgtgcaataa gatgagtgaa aaagcgacaa aaaaatgccc attctgcaat
    17221 gaattaatcc atattgaagc cgtcaaatgc cggttttgca aagagtttct cgaagataat
    17281 aggggccttc cagtatcact tcattcccga cgggcagttg gcgggaaagc agggctacaa
    17341 ttgtctgccg aacaagacag aaaaacaaga gatgagatgt ctaattctaa caaaaatgac
    17401 tcttatgttc tgtttgaggc atcgccatcg ttatgggcgc tggtcggtac gtatgtcgaa
    17461 tcagcaattt ttttggcaat cgctattatt ctgatgctgc atcctgtcag caaactggct
    17521 gcatacaaga taattccagt tgttgctgtc gagaaaatac agattgggga cacaattgcc
    17581 aagtacgggg gtatgggtct tgcgggcata gtcgtagtca atgttcttta tcagacaatt
    17641 cggctaaagc ggattcgtta tgaagtcaat cctgaccgta tagagtttac acggggtgtt
    17701 ttcagccgga aaattgacaa tcttgatatg tttcgggtgg ttgatataaa acttcatcga
    17761 agtcttctgg actgcatcac gggagtcgga tctgtcacgc tggtgaccaa agatgaatct
    17821 gatcctgtgt ttgaatttga aaaggttgcc aatcctaaag ctctgtacga tgtcattaaa
    17881 aaggcttctc tggatgctga ccgcagacag ggtgtaatcc acattgatta aatacttcct
    17941 ttgccgataa aagcggttta ataaagccgg cccgggcgga ataaaatccc ccgggccttg
    18001 tctcaaagcc gtggcgggct aggttatctc aaatgcaatt ttcgacagtc aaaaatcagg
    18061 cagcccgatc cctgtgaggt tctcatatac tcctcgtcgt taattttttg ggcgctggct
    18121 tcctttgctc gcggctgcct gaagcaaacg tccttgttcg cccggatttt tatacttctt
    18181 gagaaagctg tgtttttaaa gagcggcata ttgctgagcg acatcggcaa gttgacggtc
    18241 ggcctcgagc agtgcttcca tatccgccgt tacgaggtgg cgaatcaggg ttttgcgaat
    18301 acgcagcaga ccaagccgca aaattttttc ctcaacagtc ccggcaatag tacgcgccat
    18361 cagccgatcc atccgatagc ggctaaccaa atcttcgacg attcggcttt tatcccattg
    18421 gagccactgg ctatctacca cttcaatacg atgccgatgc agcgagcggc agagatggtg
    18481 atgctgaagt gcttcagccg cttcttcgaa acccgctccg gacaggacag ataaaatcat
    18541 taaatgaatc tgctctgcag tcagttgccc aagtgaaggc tgctgataga gaaaggcggt
    18601 aatcgcctcg gccagcattt cggctatatc caaaccgtct cttccggcac cggccaaggc
    18661 cctatgcagc gtgccgagaa ccttggtgtg caggtatggc tctgttgaac cgtctgcctt
    18721 tatcactcgt atttgcatcg attgagtcct ttcaatcgct gccgcactaa tcagacatta
    18781 aacaggtgcg gctgatctgc attgtcgggc tcccgaatgg cttggctgac ttcatccagc
    18841 agagcgccgg catctgtaaa atcccgatag acactggcaa aacgaatata tgctacctta
    18901 tccactttgc ggagaaatcg cattgcctgc tcgccaagaa acaaagaaga gacttcttta
    18961 tcgtaattgc ggaagacctc ttcttctatg cggtcggcaa tgtcctgaat tttttcagct
    19021 gaaaccggac gcttataaca ggctttctgg aggccggcaa caagcttatc acgatcatag
    19081 ggaacccgcg ttccgtcctt tttaataacg cttaatttga aggcttcgcc gattttttcg
    19141 taggtggtaa aacgccgtcc gcaaagcagg cactcacgac gccgccgaat gatacgtccg
    19201 ccttcgctcg accgggaatc aatgaccttg tcgttgtctt ctttacaaaa cggacatctc
    19261 ataaccactt ccgcgctaat ggggattact atatattcat ttttgaatat attatcatca
    19321 atatatagtg tgtcaatgga aaaaataaga attttaggat aggcaaggaa taattttcag
    19381 ttaactgatt tgcatcgatg aaaagcaaac tca
//
LOCUS       DAOTBO010000048        19348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2323_length_19348_cov_5.162805, whole genome shotgun sequence.
ACCESSION   DAOTBO010000048 DAOTBO010000000
VERSION     DAOTBO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 19348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19348
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_19348_cov_5.162805"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="PLE88_07035"
     CDS             <1..2235
                     /locus_tag="PLE88_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOM76255.1"
                     /translation="HNYVGTEHILLGLLRETEGIAAQVLMNLGLKLEDVRQEVLNLLG
                     AGVEDNAYQSMGMKMSPAAAQAAKAAKSKTPALDSFGRDLTELAAKGELDPVIGRETE
                     IERLVQILCRRTKNNPVLLGEAGVGKTAIVEGLAQRIVSHEVPELLKDKRLVVLDLAM
                     MVAGTKYRGQFEERIKAVINEVRRAGNVLLFIDELHTLVGAGGAEGAIDASNVLKPAL
                     ARGEVQCIGATTFDEYRKYVEKDAALERRFQTIMVDPPSKEETVEILKGLRDRYEEHH
                     RVHFTDEALYQAVELSSRYISGRCLPDKAIDVIDEAGARVRLKNMTPPPDLAEFEARI
                     EKLQSEKDEAVRNADYERAAALRDDIQRILDEKAEMQRQWQEKNNQTIGVVDAEVVAE
                     VVSKMTGVPLTRLEKEEAQKLLELEAELHKTVVSQDEAINALAKAVRRSRSGLKDPNR
                     PMGSFIFIGPSGVGKTLLARALAEFMFGDADALIQIDMSEYMEKHNVSRLVGAPPGYV
                     GYEEGGQLTERIRRRPYAVLLLDEIEKAHSDVYNMLLQIMEEGRLTDSFGRHVDFKNV
                     ILIMTSNIGAELIKNQSGFGFGKRTEQANYEKMKELLDKEMERHFRPEFLNRLDAQIV
                     FRPLTRQDLHTIVDYELNKVFKRLLEHHLHLELDEAAKEFLIDKGYNPEFGARPLRRA
                     IERYIEDPLSENLLRGKFKGKSLIRITVQDEQNLRFEGVDAPAPLDASNVVVHSGPET
                     QSAQ"
     gene            2284..2670
                     /locus_tag="PLE88_07040"
     CDS             2284..2670
                     /locus_tag="PLE88_07040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOM76256.1"
                     /translation="MDSTFFDKDAFSHHCGIRLLDVKPGYAAAEMPIEPKHLNSVGIV
                     HGAAVFALADAVFAAASNSHGYVAVAVNISISYIKAVSEGVLRAEAQENNPRGRMGSY
                     TITIRDQQGDLAAVFQGLAYRKFDKL"
     gene            2787..3320
                     /locus_tag="PLE88_07045"
     CDS             2787..3320
                     /locus_tag="PLE88_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76257.1"
                     /translation="MTNKKIGLVFTASNLLLLAVLLLLVSAVWAADTATAPAASGSGA
                     VSAPADVPTRAMRQAGAAQRRSGAAADRELMYQDATARRMAEHRAQIAELEEIKKVAQ
                     EENASRTVEAIQKLIEKKDSQFKQSLEQFERQRRARLEQIQERTAALRAREGQTDAGA
                     DKNKPQNAAGDKADKDN"
     gene            complement(3382..4443)
                     /gene="ispG"
                     /locus_tag="PLE88_07050"
                     /gene_synonym="gcpE"
     CDS             complement(3382..4443)
                     /gene="ispG"
                     /locus_tag="PLE88_07050"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOM76258.1"
                     /translation="MIQRRPTKTVCIGTLRLGSGHPILVQTMTKVFTTDVPACLRQIG
                     QLQKAGCGLVRIAVPTRADTQALAQIIAKTSMPIAADIHFSPDRAVEAIEAGAVKIRL
                     NPGNIKDKNSIRRIIDCAKAHNTAIRIGVNEASIRDLQHDVPPARRTSLMLKEMKAYI
                     RMFEKAGFDNLVLSAKSADTVRTIEVNRAIAGAFDYPLHIGLTHAGLPEDAIIPSAAA
                     IGTLLAEGIGDTIRVSVAGNPVREIEIARQILQALGLEEQLSPHLVVCPTCGRCRIDV
                     AALARKVKRLLSGIRKPLHVAVMGCIVNGPGEAADADIAVCAAEGKGYLYRRGQKIAV
                     VQEADLLDALAREIASAAQ"
     gene            complement(4474..4983)
                     /locus_tag="PLE88_07055"
     CDS             complement(4474..4983)
                     /locus_tag="PLE88_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76259.1"
                     /translation="MPIQFHCEACKKKIKAPDTAGGKWGSCPYCKHRCYIPLPKQDDE
                     PELKLVPIDPREETSVEELMRETKTLTQTILLQNSIPEETGRTDGSSRTAQEKDILKQ
                     CILYLRQMADGELGPAARTLSELKKNKKTAVRLLSSMGRAERPEPELADLSDGVLQGL
                     IRDACNKLS"
     gene            complement(4995..5360)
                     /gene="queF"
                     /locus_tag="PLE88_07060"
     CDS             complement(4995..5360)
                     /gene="queF"
                     /locus_tag="PLE88_07060"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOM76260.1"
                     /translation="MAEKPQLEVFDNPRPQRNYTITIRCPEFTSVCPKTGQPDFGTII
                     IEYIPDVLCVELKSLKFYLQSFRSRGIFYEFLTNEILDDLTEAVKPRWMRITSQFTPR
                     GGITTEVRAEFTAQKGTNT"
     gene            5441..6133
                     /gene="queC"
                     /locus_tag="PLE88_07065"
     CDS             5441..6133
                     /gene="queC"
                     /locus_tag="PLE88_07065"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOM76261.1"
                     /translation="MGLKKAVILLSGGLDSVTTLAIAKKMDFSCYALTFQYGQRHFFE
                     ISCAQKAALALGAADHRIIKIEPEVFAGSALVGRQQEVPKDRPMEDGTIPATYVPARN
                     TIFLSYALAWAEVLGAWDIFIGVNAMDYSGYPDCRPAFIEAFEKMANLATAAAVEGKG
                     RYTIHTPIIRMSKAQIIQQGTALGVDYSLTHSCYDPDAQGRPCGRCDSCRLRLKGFAQ
                     AGLTDPLKYPCL"
     gene            6279..7619
                     /gene="fucP"
                     /locus_tag="PLE88_07070"
     CDS             6279..7619
                     /gene="fucP"
                     /locus_tag="PLE88_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOM76262.1"
                     /translation="MAELNVMHYDASGKPKVIPHGIIGSFILVTFLFSLWGFANDITN
                     PMVAAFKNILLISNFESSLVQFAFYGGYCVMAVPAALFIKRFSYKTGILLGLALYAAG
                     CLMFIPSGQMMAFWAFLLSYFVMTCGLSFLETSANPYILAMGPEETATRRLNFAQSFN
                     PMGSLIGMLVAKNFILANLDKTDEAGRRVLRETAPEQFMALCKHDLDVIIGPYMMLGL
                     VVLATFAVFAFARLPRTQGEDDKQINFWPTLGRLVRNAKYVEGVIAQAFYVGVQIMCW
                     TFIIQYAGNELGIDKPTAQLYNMVAMIIFVSSRFICTFLLKYFSPGGLLMSLAIAGFA
                     LICGTIFIRGMMGLYCLVGVSACMSLMFPTIYGIALKGLGDDAKLGAAGLIMAIGGGC
                     LMPPMQGWIMDQNAFNLGFMTLSSTRASFVLPLICFVVIAIFGFRTSNVHGHKY"
     gene            7653..8099
                     /locus_tag="PLE88_07075"
     CDS             7653..8099
                     /locus_tag="PLE88_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004114069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOM76263.1"
                     /translation="MLKHIPAVLSPDLLKLLMEMGHGDELVIADGNFPAASIARQTVS
                     QQLIRLDGHGVPVVLEAVLRLFPLDRYVPKPAALMEVVPGDKVETPIWQEYRRIIKNS
                     GEVFDDFEYIERFAFYERAKKAYAVIATSESALYANIILKKGVVVA"
     gene            8109..8309
                     /locus_tag="PLE88_07080"
     CDS             8109..8309
                     /locus_tag="PLE88_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76264.1"
                     /translation="MIDPARMNRFNPNPEQAEAAYPVRQLEWDGKEYGSPLVRLQGAE
                     KTIENAASAAYDTCLCEVLTIL"
     gene            8324..9241
                     /locus_tag="PLE88_07085"
     CDS             8324..9241
                     /locus_tag="PLE88_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76265.1"
                     /translation="METDSPRPQNIIETTDALEAVSACQSMKNALFTIILLGLLLCQI
                     IFWLHHFGLIKGSQTSEPQPAVSRCNSGASFGPLLLTAAIAQEQEKVSPAGAQTAELV
                     GQVIDKVESGEKAPAQKAVPLVVEKEATTAPSDTAAAEAAPKSEPAAKDESDTNIKTL
                     LKEELALFHISPRFAKGLIAVCNFAILTGAILYSLTLLVCVKISLAGRLGGMNHIVRA
                     FFVSLFLLVFLIPWQVVLPKVLLGAVWLPGELLDGRWDKAGDSVVWAVMLYLRFCGLW
                     LIALWFLLWAQIRSAKWARAILRRLGVVR"
     gene            9246..10289
                     /locus_tag="PLE88_07090"
     CDS             9246..10289
                     /locus_tag="PLE88_07090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM76266.1"
                     /translation="MLICQSVYGTIQPFTSAGQSIIADSVRVFVWGAGLVCRQYARID
                     ERDGMKEQIAYIGMGIMGAPMAVNLLNGGYSVVVHTRTPQKAQPVLDRGARWADSPAE
                     AARHADVVITCLPDTPDVQKVLLGENGVIETARRGTVCIDMSTISPQITQQIAAALAQ
                     KGLVMLDAPVSGGQSGAVEAKLSIMVGGPKEAFERVRPILACLGRAITHCGPAGFGQL
                     TKLANQVMVIHTIVSIAEGLAFAEQAGLDLQTTWEAACSGAAYSHSLKVLGAKILAGD
                     MKPAFKAVLQLKDLRLVMETARQIGQPLPATALAAELLSVLPAIGRGEDGTQALIDVI
                     RHLRMPKESRTES"
     gene            complement(10360..11739)
                     /locus_tag="PLE88_07095"
     CDS             complement(10360..11739)
                     /locus_tag="PLE88_07095"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HOM76267.1"
                     /translation="MSKKNKGIALLIGLIFVAVFSALSAAMFMMSTANARIARNLHSV
                     NEARSCAESGLEVIRYYAQKITLPGSTLPENLFSVFRTSLESILPSNLNASFDSDSQT
                     LSIGSASAPVMLDSSRSFYAEISPATGSIQIRVKGQAGGIGRTILGGFSFGIDENTKS
                     AFDFGVATKGPLSLKSITLDGVNLRVESDVYIESSATNSALTIQNAQIAGDVKIVNPS
                     AFVTMSGGQSSIGGEKGAAAITNHVEIGVSGAEFPIPNPAHFERYINGITIDSSNKSL
                     YSNNATLENVRIAAGTNPKFTGNTIIKGVLYIEQPNVVKFGGNAQITGVIVGNGSLSD
                     NSATNQLNFTGTVVSTSVANLPQTEQYAGLHSETGTFIMAPGFAVSFGGNFGTLNGCI
                     AANGVRTYGSAGGIIGGSIVNYSTEPMRLEGSDLFFNRSGITDIPAGFEINSDISIYY
                     NPSLYDEVP"
     gene            complement(11972..12826)
                     /gene="lipA"
                     /locus_tag="PLE88_07100"
     CDS             complement(11972..12826)
                     /gene="lipA"
                     /locus_tag="PLE88_07100"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOM76268.1"
                     /translation="MNKGQQRLPPWLKRRLGAGQTFSHTHQTLQGLGLETICTNANCP
                     NRGDCWSRGTATVLILGNICTRNCRFCSVAHGKPLPPDPTEPDRIAQMALRMGLKYLV
                     LTSVNRDDLPDGGAGHFRDCIQTCRRVMPALEFELLVPDFRNCQEQAVAILSDALPFV
                     FGHNIETVPALYPTVRPGGDYQRSLELLKIAKKVWPHIETKSSLMLGLGETDQQVLAV
                     LNDLRAVGCDRLALGQYLKPDKYSLDVAEFIRPEKFDEWANLARQLGFRWVHSSPFTR
                     SSYHAEDK"
     gene            complement(12831..13463)
                     /gene="lipB"
                     /locus_tag="PLE88_07105"
     CDS             complement(12831..13463)
                     /gene="lipB"
                     /locus_tag="PLE88_07105"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOM76269.1"
                     /translation="MAVLEIIDAGLMPYAQALALQTELCLKRQQEQIPNTVIITEHPP
                     VITLGARKTENKLRAPAEDIRSCNIELVQVGRGGGTTAHNPGQMVLYPVIKLKTLRLD
                     ITGYIRSLESIGIDLLEGLGVSCCRRKGFPGLWVGEKKIASVGVQIKKWVSFHGMAIN
                     INNDLNIFDWIVPCGLDAVEMTSVYKETGTLSDMAAVKNKLADLCRTYWT"
     gene            13670..14749
                     /locus_tag="PLE88_07110"
     CDS             13670..14749
                     /locus_tag="PLE88_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOM76270.1"
                     /translation="MGITLKKEYTWSLVVPTSMGVRLTPTDAQPMHCGRTLLMQATSA
                     ETNVASVSSYLGLPVKVLTTFVKDSPIARFIKDDLASRHIVYEGKEVPQGGPWGYRHQ
                     FNLADCGFGSRGPRVHNDRAGEVGRTLNVKDFDLQRLFGKEGVQIVHLSGLIAALSPD
                     TGQFCLELARTAKQFDTRISFDLNYRASFWKGREAELAAIFKEIASVADILVGNEEDF
                     QLCLGIEGPEAGGKDIAAKIDSFKAMIGCVKKAFPNAQVYGTTLRQVISANCHLWGAI
                     MAEGDNWHIVEPREIAVLDRIGGGDGFVGGMLYAILKGWEPQKWIQFGWATGALAVTM
                     LTDYAQPADEEQVWSIWQGNARVKR"
     gene            14814..15977
                     /locus_tag="PLE88_07115"
     CDS             14814..15977
                     /locus_tag="PLE88_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HOM76271.1"
                     /translation="MLKTVWNLIRTGVIVVGVLLSFFAVLEVLRTYQTLHAFHPWAGG
                     LFLAAAVGLVIWLAVYVWINLAAFPPALNPPRIEDFAKASQHQLKQHLRYLRRFLQRL
                     QENPGISEDKRGLIGTALDRISQTLSAPSSAEAMRMAIEAVQADAVKPILSDLDELAA
                     RQVRDSMRDIMIGVTLSPYKSADLLIVLYRNLVMVMRIVRIYRTRPALSELLGIFSDI
                     VNVIATVNYINMGKNLIEGLGSRVPGIGRFVDDIAQGIGAGFMTTITGHAAIDRCRSF
                     REWNAAEAKRHLLSHVGDFYNDVKDIFFKDVWGGIRSRIGAAGDNKDKVADALNETGS
                     LLDSFIRVPAKAVGAAGQAVAGTTNKGFELVGRKIIKAGTALTKPFRKTKPTL"
     gene            complement(16464..17177)
                     /gene="tsaB"
                     /locus_tag="PLE88_07120"
     CDS             complement(16464..17177)
                     /gene="tsaB"
                     /locus_tag="PLE88_07120"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOM76272.1"
                     /translation="MTDSQLILAIETSGRTGSAAIGRDDTLAAQIVFSGFMRHSAELF
                     GAIEELLRQAGAVPSDIRQVYITGGPGSFTGLRIAVTAAKMLAFALKSRIIAADSMDV
                     LAENGTDYILQTGQMLDSIVTVLDAKKDFFYTAVYDWTGNGWEKKIGTEMTTAEQLLD
                     RLAQSGRKNVGLLGEGLLYYADKFKSPFTCLLDKAFWPATAGGLYRVGRRLAALNRFA
                     DPLSLTPFYIRKPDAVMKK"
     gene            17394..>19348
                     /locus_tag="PLE88_07125"
     CDS             17394..>19348
                     /locus_tag="PLE88_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76273.1"
                     /translation="MKTLYFWRSYKHAGIAAMLVLCGMWCSLLWAQPSNIDQAKRAKI
                     DTTIRIAQEQMKRGFYQQAQTQLNTLLTSSEYTNYLSERQHQTITALLNEISTALQER
                     TKITQMLQQSDTLASQGKYTEALGLLGQIKDSPFASEQERQMIRDLYQDVSAKQLQEQ
                     QKWQSLYDASTAAYKAGQYEQARQGFVQILEAGASVKGDKTAQEYIQLIDSAAAQPAG
                     TGQADSAEPMVPLLETPAAGTSSAQEAIEPLDLMPQPDASAQTVATVSVQVQEQKERS
                     YLEVVRQKRAVQVDYTTAIVKDAIEKAQQALASNQFEQAQQALRRASSTVEANKMLLG
                     DAFYADYKAQLTNLEQKTEEARQAYQQQQETQRQQQADELNAQIRQTMEEQRARAVKD
                     YLARAYAFLKEQRHEDALGQLEQLLAIDPLNQEAQILKQTLEHMDHYIKQRQIQEESD
                     REEIALLLDVKRRAIPYADEFNFPHNWKEISARREQAVKEAQTPEDMAVNELLDKTVD
                     LSMLTEDTTLAEAIELLRNSVDPPLPIVVYWSDLSQNAFVEKTTPINMSGEGLRRVVL
                     RTALSRVLQAVGSGGFAELAYVVQEGTVTVATRDSLPANYVTEIYDVADLLNPPANYD
                     EDYSSSGYGGGGYGGGGYGGGGYGGGGG"
BASE COUNT         4406 a         4747 c         5577 g         4618 t
ORIGIN      
        1 cataattatg tggggacaga gcatattctg ctggggctgc tgagggagac agagggcatt
       61 gctgcacagg ttctgatgaa tctgggactg aagctggaag atgtccgtca ggaggttctc
      121 aacctgctgg gggccggcgt agaagacaat gcctatcaat cgatggggat gaagatgagt
      181 cctgccgcag cgcaggcggc caaagcggcc aagagcaaaa ccccggccct ggacagcttc
      241 ggcagggatt tgaccgagct ggcagcaaag ggggaactgg atcctgtcat cggccgggaa
      301 acggagattg agcgtctggt gcagattctc tgccggcgca ccaaaaataa tcctgtgctt
      361 ttgggagagg ccggcgtggg aaagacggcc attgtcgagg gactggccca gcggattgta
      421 tcccacgagg ttcccgagct gctcaaggac aaacggctgg tcgttctgga tttggcgatg
      481 atggtggccg gcacgaaata ccgcgggcag tttgaggagc gcatcaaggc ggtcatcaac
      541 gaggtgcgcc gggcggggaa tgtgctgctg ttcatcgatg agctgcatac gctggtgggg
      601 gccggcgggg cggaaggcgc tatcgatgca tccaatgtgc tcaagcccgc tctggcacgc
      661 ggcgaggtgc agtgcatcgg tgcgacgacc tttgatgaat accgcaagta tgtggaaaag
      721 gatgccgctc tggagcgccg cttccagacg ataatggtgg atccacccag caaagaggaa
      781 acggtggaaa tcctcaaagg gcttcgcgac cggtatgaag agcatcaccg ggttcatttt
      841 accgatgagg cgctgtatca ggccgtcgag ctgtcgagcc ggtatatttc gggccggtgc
      901 ctgccggaca aggccatcga tgtaatcgac gaggccggcg cccgtgtgcg tctgaaaaac
      961 atgacgcctc cgccggatct ggcggaattc gaagcccgca ttgaaaagct tcaaagtgaa
     1021 aaagacgaag ctgtccgcaa tgccgattat gaacgtgctg cagcgctgcg ggatgacatc
     1081 cagcggattc tggatgagaa agccgagatg cagcggcagt ggcaggaaaa gaataatcag
     1141 accattgggg ttgtggatgc cgaggtggtt gccgaagtgg tcagcaaaat gaccggtgtg
     1201 ccgctgacgc gtctggaaaa ggaagaagcc cagaagctgc tggagctgga agccgagctg
     1261 cacaagacgg ttgtttcgca ggatgaggcg atcaacgcac tggccaaggc ggttcggcga
     1321 agccgcagcg gcctgaaaga ccccaaccgt ccgatgggga gctttatctt catcggcccc
     1381 agcggcgtcg gcaagacgct gctggcccgt gcgctggccg agtttatgtt cggcgatgcc
     1441 gatgcgctca ttcaaattga tatgagcgaa tatatggaaa agcacaatgt cagccggctg
     1501 gtcggggcgc ctcccggcta tgtgggctat gaagagggcg gccagctgac ggagcgcatc
     1561 cgccggcgtc cctatgccgt gctgctgctg gatgaaatcg aaaaggcgca ctcggatgtc
     1621 tataatatgc tgctgcagat tatggaagaa ggccgcctga ccgattcttt cggccggcat
     1681 gtggatttca aaaatgtgat tctgattatg accagcaaca tcggtgcgga actgatcaag
     1741 aatcagtccg gcttcggctt cggcaagcgg accgaacaag ccaattatga aaagatgaag
     1801 gaactgctgg acaaggaaat ggagcggcat ttccgtccgg aattcctcaa ccggctggat
     1861 gcccagattg tcttccgccc gctgacgcgg caggacctgc atacgattgt cgattatgaa
     1921 ttgaacaagg tattcaagcg gctgctggag caccatctcc atctggagct ggatgaggcg
     1981 gccaaggaat tccttatcga caaaggctac aaccccgaat tcggggcgcg tccgctgcgc
     2041 cgggcgattg agcgctatat tgaagacccg ctttcggaaa atctcctgcg cggcaagttc
     2101 aaaggcaaga gcctgatacg gattaccgtg caggatgaac aaaatctgcg gtttgaaggc
     2161 gtggatgctc cggctccgct ggatgcgtct aatgttgtgg tgcattcagg cccggagacc
     2221 cagtctgcgc agtgatgcgc cggaaattac aggcagacag tttgacggaa acggcccaac
     2281 ggcatggaca gtacattttt tgacaaggat gcattttcgc atcattgcgg aattcgcctg
     2341 ctggatgtca agcccggcta tgcagcggca gagatgccca ttgaacccaa gcatctcaac
     2401 agtgtcggga ttgtgcacgg ggctgctgtg tttgcactgg ccgatgcggt ttttgcggcc
     2461 gcttccaatt cgcacggcta tgtggcggtg gcggtcaata tcagcatttc gtatatcaag
     2521 gcggtctccg aaggtgttct gagagcagag gcgcaggaaa acaacccccg cggccggatg
     2581 ggcagttata caattaccat acgggaccag cagggcgatt tggcagccgt gtttcagggt
     2641 cttgcatatc ggaaatttga taagctgtaa cggcttttcc ccggcgcaaa aaagagtcaa
     2701 aaatactttt ggaatctgtt ttttggccga caatgaggat tagacaggta aataacggca
     2761 gcaattatca gtcacaggag aaaaaaatga ctaacaagaa gattggactg gttttcacag
     2821 caagtaatct tttgctgctg gcggttcttt tgttgctggt gtctgcagta tgggcagcgg
     2881 atacagcgac tgcgccggcg gcttcgggtt ccggtgcggt ttcggctccg gctgatgtgc
     2941 cgacgcgtgc gatgcgtcaa gccggcgcgg cacagcggcg ttcgggcgct gctgccgatc
     3001 gggagctgat gtatcaggat gcgacagccc gccggatggc cgagcatcga gcgcagattg
     3061 ctgagctgga agaaatcaag aaagtggcac aggaagaaaa cgcttcccga acagttgaag
     3121 ccatccagaa gctgattgaa aagaaagatt ctcaattcaa gcagtcgctg gagcagtttg
     3181 aacgtcagcg gcgtgcccga ttggagcaga ttcaggaacg cacagcagcc ctgcgtgccc
     3241 gggaagggca gacagatgct ggtgccgaca aaaataagcc gcaaaacgcc gccggcgata
     3301 aagccgataa agacaactaa accggccgat ttccagacag tcaggcaggg ccgagacggc
     3361 agcatttcgg tcctgctttt tttattgtgc ggcggatgct atttcacggg caagggcatc
     3421 cagcagatcg gcttcttgca caacggcaat tttctgtccg cgcctgtaaa gatacccttt
     3481 gccttctgct gcgcagacgg caatgtcggc atcggcggct tcaccggggc cgttgacaat
     3541 gcagcccata acggccacat gcaggggttt gcggatgccg ctgagcagcc gtttgacctt
     3601 tcgtgccagt gcggccacat caatccggca gcgtccacag gtggggcata cgaccagatg
     3661 gggcgatagc tgttcttcca gcccgagggc ctgcagaatc tgccgggcga tttcaatttc
     3721 ccgaaccgga ttgccggcta cactgacgcg gatggtgtcg ccgatgcctt ctgccagcag
     3781 cgtgccaatc gccgccgccg aaggaatgat tgcgtcttcg ggcaggccgg catgggtcag
     3841 gccgatatgc aggggataat caaatgcgcc ggcaatggcg cggttgacct caattgtccg
     3901 tacggtatcg gcgcttttgg cgctgagcac aagattgtca aagcccgcct tttcaaacat
     3961 ccggatgtag gccttcattt ctttgagcat caggcttgta cgccgtgccg gcgggacatc
     4021 gtgctgaagg tcgcggatgc tggcttcatt gacgccgatg cggatagccg tgttgtgggc
     4081 tttggcacag tcgataatcc ggcggataga gtttttatct ttgatgtttc cgggattgag
     4141 ccggattttg acggcaccgg cttcgattgc ttcaacggca cggtccggcg aaaaatggat
     4201 atcggcagcg atgggcatgg aagtttttgc gataatttgg gccagcgcct gcgtgtcggc
     4261 acgtgtcggc acagcgatgc gcaccagacc gcagccggct ttttggagct ggccaatctg
     4321 ccgcaggcac gccgggacat cggtggtaaa gactttggtc attgtttgaa caaggatggg
     4381 atgaccggaa ccgagccgca gtgttccgat gcagacagtt tttgtgggtc gccgctgaat
     4441 catgactgat gctgaaatgg gtggatagaa tgtttaactg agcttattgc aggcatcgcg
     4501 aatcagaccc tgcaggacgc cgtcggacaa atccgccaat tccggttccg gccgttcagc
     4561 ccgtcccatc gatgaaagca gacggacggc cgtttttttg tttttcttca gttccgacag
     4621 tgtgcgggcg gccggtccca gctcgccgtc ggccatctgt cgcaggtaaa ggatgcactg
     4681 tttgagaata tctttttcct gtgcggtgcg gctgctgccg tctgtgcggc cggtctcctc
     4741 cggaatggag ttctgaagca gaatggtctg ggtcagtgtt ttggtttcgc gcatcaattc
     4801 ctccacgctg gtttcttccc gcggatctat cggcaccagt ttcaattccg gctcgtcatc
     4861 ctgtttggga agggggatat aacagcgatg cttgcagtac gggcagctgc cccatttacc
     4921 gccggctgtg tcgggggctt ttatcttttt tttgcatgct tcgcaatgga actgaatagg
     4981 cataagcggg tctcctaagt gttggttccc ttttgggccg taaattcagc acggacctct
     5041 gtcgtaatgc cgccgcgcgg ggtaaactgc gatgtgatgc gcatccagcg gggcttgaca
     5101 gcttcggtca aatcatcaag gatttcgttg gtcagaaatt cgtaaaatat gcctcggctg
     5161 cggaaactct gcaggtagaa tttgaggctc tttaattcaa cacaaaggac atctggtatg
     5221 tattcaatta taatagttcc aaaatccggc tgtccagtct tggggcaaac actggtaaac
     5281 tccgggcagc ggatagtaat agtataattg cgttgcggtc gaggattatc aaatacttcc
     5341 agttgcggtt tttcagccat tctgcttgtc cttttgagta atactgctta tactttcgat
     5401 ataattttag caaaacttgg cagctttgca aggaaagaaa atgggattga aaaaagcggt
     5461 tatactgctc agcgggggac tggactctgt tacaacgctg gcgatagcaa aaaaaatgga
     5521 tttttcctgc tatgcactga ctttccaata cggtcaaagg catttttttg agatttcctg
     5581 tgcccaaaag gcagcgctgg cgttaggggc tgcggaccat cggataataa aaatagagcc
     5641 ggaggtattt gccggctcgg cccttgtcgg ccggcagcag gaggttccca aagaccggcc
     5701 gatggaggac ggcacaatac cggcaaccta tgtgccggcg cgcaatacaa tctttctcag
     5761 ttatgccctt gcttgggcag aagtgctggg agcgtgggat atttttatcg gtgtaaacgc
     5821 aatggactac agcggttatc cggattgtcg gccggcattt atcgaagctt ttgaaaagat
     5881 ggccaatctg gcaaccgctg cggctgtcga gggcaaaggc cgatacacaa ttcatacgcc
     5941 gattattcgg atgagcaaag cccaaatcat ccagcagggg acggctttgg gggtggatta
     6001 ttccctgacg cacagttgtt atgacccgga tgcacaaggg cgtccttgcg gccgctgtga
     6061 ttcctgccgg ctgcgtctga agggctttgc tcaggcagga ttgactgatc ccctgaaata
     6121 cccctgtttg taaagacctt gaggaatggg caggcagaga agctgtctgc aggggggatt
     6181 tttattgctc caaacgataa agttgttaaa ctaatctgct tatataggga gtttgcggcg
     6241 cctgagaaca ggaacatttt agtgaaaggt caacgaatat ggcggagttg aatgtcatgc
     6301 attatgatgc gtcgggaaag ccgaaggtta ttccgcacgg tattataggg tcctttattc
     6361 tggtaacttt tctgttttct ttgtggggct ttgccaacga tattaccaat ccgatggtgg
     6421 cggcgttcaa aaatattctg ctgatcagca attttgaaag ttcactggtt caatttgcct
     6481 tctacggcgg ctattgcgta atggctgttc cggcggcctt gtttatcaag cggttcagtt
     6541 ataagaccgg cattctgctg ggtctggcgc tgtatgcggc cggatgcctg atgtttatcc
     6601 cgtcggggca gatgatggcc ttttgggctt ttctgctgtc gtattttgta atgacctgcg
     6661 gcctgtcctt tctggaaacc agcgccaatc cctatattct ggcgatgggg ccggaggaga
     6721 cggcaacccg ccgtttgaat tttgcccagt ctttcaatcc gatgggctcg ctaataggga
     6781 tgctggtagc caaaaatttt attttggcca atctggacaa aacggatgaa gccgggcggc
     6841 gggtgcttcg ggagacagca ccggagcagt ttatggctct ctgcaagcac gatttggatg
     6901 tgattatcgg cccgtatatg atgctggggc tggtggtgct ggcgacattt gcggtatttg
     6961 cctttgcccg tctgccacgc acccaaggcg aggacgacaa gcagattaac ttctggccga
     7021 cattgggccg gcttgtccgc aatgccaaat atgtggaagg agttattgca caggcattct
     7081 atgtgggggt acagattatg tgctggacat ttattatcca gtatgcaggc aacgaactgg
     7141 gtattgataa gccgacagcg cagctgtaca acatggtggc aatgattatc ttcgtcagca
     7201 gccgctttat ctgcaccttc ctgctgaaat acttcagccc cggcggcctg ctgatgtcgc
     7261 tggcaattgc cggattcgcc ttgatttgcg ggacaatctt tatccgggga atgatggggc
     7321 tttattgtct ggtaggcgtt tcggcgtgta tgtcgctgat gttcccgacc atttacggca
     7381 ttgccctcaa agggctgggg gatgatgcca agctgggggc ggcggggctg attatggcta
     7441 tcggcggcgg ctgtctgatg ccgccgatgc aggggtggat tatggaccag aatgcattca
     7501 atctgggctt tatgacgctg tcgagcacac gggcttcgtt tgtcctgccg ctgatttgct
     7561 ttgtcgtgat cgctatattt ggttttcgaa cctccaatgt ccatgggcat aaatattgac
     7621 agaccaaccc acttcaacga gaggataaac caatgctcaa gcatatacct gctgttttaa
     7681 gtcctgacct gctgaagctg ctgatggaga tggggcacgg cgatgaactg gttatcgccg
     7741 acggcaactt ccctgccgcc tcgattgccc gccaaacggt ttcgcagcag ctgatccggc
     7801 tggacggcca cggggttccg gtggtgctcg aagcggtttt gcggctgttt ccgctggacc
     7861 ggtatgttcc gaagccggcg gccctgatgg aggttgtgcc gggtgataag gtcgaaaccc
     7921 ccatctggca ggagtaccgc agaatcatca aaaacagcgg tgaagtgttc gacgattttg
     7981 agtatatcga acgctttgcc ttttatgagc gggctaaaaa agcctatgcg gttattgcca
     8041 ccagcgaatc ggctttgtac gccaatatca ttctcaaaaa gggtgttgtt gtcgcctgaa
     8101 ctgcaggatt gattgatccg gcacgaatga atcgcttcaa tccgaatcca gagcaggcgg
     8161 aggcggcata cccggttcgg cagcttgaat gggacggcaa ggaatacgga agtcctctgg
     8221 ttcggctgca gggggcggaa aaaacaattg aaaatgcagc ttctgcggct tatgatacat
     8281 gtctatgtga agtgctaaca atcctttaaa tggagaacca ataatggaaa ccgattcccc
     8341 aaggccccag aatattatag aaacaaccga tgcgctggag gccgtcagtg cgtgtcagtc
     8401 gatgaagaat gccttgttta cgatcattct tctcggattg ctgctgtgtc agattatctt
     8461 ctggcttcat catttcggcc tgattaaagg ttcccagaca tctgagcccc agccggccgt
     8521 aagccgctgc aatagcgggg cttcttttgg gccgctgtta ttgaccgctg caatcgcaca
     8581 ggagcaagag aaggtttctc cggctggtgc ccagacggct gaattggtag ggcaggtgat
     8641 agacaaggtc gaatccggcg aaaaagcccc ggcgcagaaa gcagttccgc ttgtggtcga
     8701 aaaagaggca accacagccc cgtcagacac cgccgccgct gaagcggctc cgaaaagcga
     8761 accggctgca aaagacgagt ctgatacaaa catcaagaca ctcctgaagg aggagcttgc
     8821 gctgtttcat atttcccccc gctttgccaa aggtctgatt gccgtctgca attttgcgat
     8881 tttgacaggt gcgatactgt attcgctgac gctgctggtg tgtgtcaaga tttcgctggc
     8941 agggcggctg ggcggaatga accacattgt gcgggcgttt tttgtatctt tgtttctgct
     9001 ggtttttctg attccgtggc aggtggttct gccgaaggtg ctgctggggg ctgtctggct
     9061 gcccggcgag ctgctggacg gccgatggga caaggccggc gattcggttg tatgggctgt
     9121 gatgctgtac ctgcggttct gcgggctgtg gctgattgcc ctttggtttc tgctgtgggc
     9181 ccagattcgt tcagccaaat gggcgcgggc gattctccgc cggctgggcg tggtacgatg
     9241 acccgttgct gatctgccag tctgtatatg gcacaataca gccattcaca agtgccggcc
     9301 aatccatcat tgccgattcg gttcgggtgt ttgtctgggg tgcgggattg gtatgccggc
     9361 agtatgcccg gattgacgaa agggacggaa tgaaagaaca aatcgcctat atcggaatgg
     9421 gcatcatggg agcgccgatg gcggtcaatc tgcttaacgg cggctattcc gttgtcgttc
     9481 atacgcgcac cccacaaaag gcccagcccg tccttgacag aggggcccgg tgggcagata
     9541 gccccgcaga ggccgcccgg catgcggatg tagtgataac ctgcctgccc gatacaccgg
     9601 atgtccaaaa ggtgctgctt ggggaaaacg gcgttattga aaccgcccgc agaggaaccg
     9661 tctgcatcga tatgtccacc atcagcccgc agataactca gcagatagct gccgctttag
     9721 cccagaaggg tcttgtgatg ctggatgctc ctgtctccgg cggccagagc ggggctgttg
     9781 aagccaaatt gtccattatg gtcggagggc cgaaggaggc gttcgaacgg gtgcgtccga
     9841 ttcttgcgtg cttggggcgg gcgattacgc attgcgggcc ggccggattt gggcagctga
     9901 ccaagctggc caatcaagtg atggttattc acacgattgt gagcattgcc gaagggctgg
     9961 cctttgcaga acaagcgggt ctggatctgc agaccacctg ggaggccgcc tgcagcggag
    10021 cggcctacag ccattcgctt aaagtgctgg gggccaagat attagccggc gatatgaaac
    10081 cggcttttaa ggccgttttg caactgaaag atttgaggct ggtgatggag actgctcgtc
    10141 agatcggcca gccgctgccg gcaacggcgc tggcggctga actgttgagt gtactgccgg
    10201 ctatcggccg aggcgaagac ggcacacaag ccctgatcga tgttattcgt catctgcgga
    10261 tgccgaaaga aagccgtaca gagtcataag acaagcacga tggctctgta cggcccggtc
    10321 cggtatatgc gcacgaaggt cggcattcaa tccgcttttt tagggcactt catcatagag
    10381 cgaaggattg taataaattg aaatatcact gttgatttca aatccggcgg ggatgtcggt
    10441 aatgcccgac cggttgaaga acaagtcgct gccttccagc cgcatgggtt cagtggaata
    10501 gttgacaatc gagccgccga tgatgccgcc ggcactgccg taggttcgga cgccgttggc
    10561 cgcaatgcag ccgtttaagg tgccgaagtt gccgccgaag gaaaccgcaa atccgggcgc
    10621 cataatgaat gtccctgttt cgctgtgcag tccggcatac tgttcggtct ggggcagatt
    10681 ggcaacactg gtgctgacga cagtgcctgt aaaattgagc tggttggttg ccgaattgtc
    10741 gctgagactg ccgttgccga cgatgacgcc ggtgatctgg gcgttgcccc cgaacttgac
    10801 cacattgggc tgctctatgt atagaacgcc tttgataatg gtattgccgg taaacttggg
    10861 attggttccg gcggcgatgc gtacattctc gagtgtggca ttgttgctgt agagactttt
    10921 gttgctgctg tcaattgtga tgccgttgat gtaccgctca aagtgagcgg gattgggaat
    10981 aggaaactct gctccgctga cgccgatttc cacatggttg gtaatcgccg cagctccctt
    11041 ttcgccgccg atgctgctct gcccgccgct catcgttaca aaggcgctgg gattgacaat
    11101 tttgacatcg ccggcaatct gggcgttctg gatggtcagt gcactgttgg tggcagagct
    11161 ttcgatatac acatccgatt ccacgcggag attgacgccg tccaatgtta tgcttttcag
    11221 cgacagaggg cctttggtgg cgacgccgaa atcaaatgct gattttgtgt tttcatcgat
    11281 gccgaagctg aagccgccca aaatggttcg cccaatgccg ccggcttgac cttttacgcg
    11341 aatctgaatg ctgcccgttg cagggctgat ttcggcataa aagcttctgg aactgtccag
    11401 catcacaggt gcgctggcag aaccgatgga caacgtttgg ctgtctgaat cgaaggatgc
    11461 attgagattg gaaggcaata tgctttcgag cgatgtccgg aaaaccgaga acaggttttc
    11521 aggaagcgtt gagccgggaa gagtaatctt ctgggcgtaa tagcggatta cttccagtcc
    11581 cgattcggcg caggagcggg cttcgttgac gctgtgaagg ttgcgggcaa tgcgcgcatt
    11641 ggcggtggac atcataaaca tagctgccga caaagccgaa aaaacagcca cgaaaatcag
    11701 cccaatcagc agcgcaatac ccttgttttt tttagacatt ttttttgcgg tgcacataac
    11761 aggtcccccc aaacagaagt ttggttaatg gctctatcta aaacatacga ttcagcggct
    11821 caaatgccaa attgaaaaag ggcttcgatg cattgtccga taatgcttcg gcaatcagga
    11881 ttacaatgct gcatcagcac catctttgag cacccgactt tgtcgattcc tgtaagctgg
    11941 cgctgcagaa gcagaaagaa ttgtccaatt tctatttatc ttcagcgtgg taggagctgc
    12001 gggtaaaagg cgaactgtgc acccagcgaa agcccaattg ccgagccaga tttgcccatt
    12061 catcgaattt ttcgggtcgg atgaattctg ctacatccag agagtattta tcgggtttaa
    12121 ggtattgtcc caaggccaga cggtcgcagc cgacagcccg caggtcgttg aggacagcca
    12181 gaacctgctg gtctgtttct cccagtccga gcatcagcga ggatttggtc tctatatgcg
    12241 gccagacctt cttggctatt ttcagcagct ccagcgagcg ctggtaatcg ccgccgggac
    12301 gtaccgttgg gtataaggcc ggcactgtct cgatattatg cccaaagaca aagggcaggg
    12361 catccgacaa gatcgcaaca gcttgttcct gacaattccg aaaatccggc accagcagct
    12421 caaactccag agcgggcata acgcgccgac aggtctgaat acagtcgcga aagtgtccgg
    12481 ccccgccatc gggcaaatcg tcgcggttta cactggtcag gaccagatac ttaagcccca
    12541 ttcgaagggc catttgagcg atgcggtccg gctcggtcgg atcgggcggc aggggtttgc
    12601 cgtgggccac agaacaaaac cggcaattgc gcgtgcagat attgccgagt atcaaaacgg
    12661 tggctgtgcc ccgactccag cagtcgccgc ggttaggaca gttggcgttg gtacagatag
    12721 tctccagccc cagtccttga agggtttgat gtgtatgact gaaggtctgc ccagccccca
    12781 gacgccgttt gagccacgga ggcaatcgct gttggccttt attcattttt ttaggtccaa
    12841 tatgtgcggc ataaatctgc cagtttattt ttaacggctg ccatatccga cagcgttccg
    12901 gtttctttat agaccgaggt catttcgacc gcgtccaatc cgcaggggac aatccagtca
    12961 aaaatgttta aatcattgtt gatattgatt gccattccgt gaaacgatac ccatttctta
    13021 atctggacgc cgactgatgc aatttttttc tccccgaccc acaggccggg aaatcctttt
    13081 cgccgacagc aggaaactcc cagcccttcc agcaggtcaa tgccgatgct ttccagcgac
    13141 cttatatagc cggtaatatc cagccgcagc gttttaagtt tgattaccgg atacaaaacc
    13201 atctgaccgg gattgtgggc agtcgttccg ccgccgcggc cgacctgcac taactcgata
    13261 ttgcagctgc gaatatcttc tgcaggcgcc cgcaatttat tttccgtttt tcgtgctccc
    13321 aatgtaatta caggcggatg ttcggtaatt atcacagtgt tggggatttg ctcttgctgc
    13381 cgctttaaac acagctcggt ctgaagggcc agcgcctgag cataaggcat caacccggca
    13441 tcaattattt caagtacagc catctcctgc attttaccag aaataccatt ttcttatata
    13501 ttgaaccagc taatatagcc gtttggcaac caaaaagcaa acctgcaaat cgcatgaaaa
    13561 catttgctca cagcgtgaga tatttcttga cagatttcat tggctgtgtc atattgacgt
    13621 cattgtttag ctcaaacagg atgaaaaaca gtcaaagagg attagtacga tgggtatcac
    13681 gctcaaaaaa gaatatactt ggagtctggt cgttcccacg agtatgggtg ttcgtttgac
    13741 cccgacagat gctcagccga tgcactgcgg gcggacgctt ctgatgcagg caaccagtgc
    13801 cgaaaccaat gttgccagtg tttcatcgta tcttggtctt cctgtaaagg tgctgacaac
    13861 atttgtcaag gacagtccga ttgcccgctt tatcaaagat gaccttgcca gccggcatat
    13921 tgtctatgaa ggcaaggaag tcccgcaggg cggcccgtgg ggctatcggc accagtttaa
    13981 ccttgctgac tgcggtttcg gcagccgggg cccgcgggtt cataatgacc gtgccggcga
    14041 ggttggccga acgcttaatg tcaaggattt tgaccttcaa cggcttttcg gcaaagaagg
    14101 tgtccaaatt gtccaccttt ccggtctgat tgccgctctt tcgcctgata ccggccaatt
    14161 ctgtctcgaa ctggcacgca cggctaaaca gtttgatacc cgcatcagtt ttgatttgaa
    14221 ctatcgggca tcgttttgga aaggcaggga agcagagctt gctgccatct tcaaagaaat
    14281 tgcttcggta gcagacattt tggtcggcaa tgaagaggat ttccagctct gtctgggcat
    14341 cgaaggtcct gaagccggcg gcaaagatat tgctgccaag attgacagtt ttaaggccat
    14401 gattggctgt gttaaaaagg ccttcccgaa tgctcaagtt tacggcacca cgctgcggca
    14461 ggttatcagc gccaactgcc acctgtgggg ggctattatg gccgaaggcg ataactggca
    14521 tatcgttgag ccgcgggaaa ttgctgtgct ggaccgcatc ggcggcggcg acggctttgt
    14581 cggcggaatg ctctatgcga tactcaaggg ctgggagccc cagaaatgga tacagtttgg
    14641 ctgggcaaca ggtgcgctgg ctgttacgat gctgacggat tacgcccagc cggccgatga
    14701 ggagcaggtc tggagcatct ggcagggcaa cgcccgcgtc aaacgctgaa agaacaattg
    14761 acagccctgt ctgcgaatcg ctacaatgcg gataatttag acaggagctg gctatgctga
    14821 aaaccgtctg gaatctgatt cgaaccggcg ttattgtcgt tggggtttta ttgagttttt
    14881 ttgccgtttt ggaggtgctg cggacttacc aaacgctgca tgcatttcat ccgtgggcag
    14941 ggggattgtt tctggcggcc gctgtcggtc tggttatctg gctggcggtc tatgtttgga
    15001 tcaatctggc agctttcccg cctgcactga atccgccccg cattgaagac tttgcaaagg
    15061 cttcccagca tcagctgaaa cagcatctgc ggtatttgcg ccgttttctg cagcggcttc
    15121 aggaaaatcc gggtatttcg gaggacaagc ggggccttat tggtacagca ttagacagaa
    15181 tcagccaaac gctttccgct cccagctctg cagaagcaat gcgaatggct atcgaggctg
    15241 tccaagccga tgcagtgaaa ccgattttat ccgatttgga tgagctggca gcccgtcagg
    15301 tgcgggattc catgcgggac attatgatcg gagtaacact tagcccgtat aaatccgccg
    15361 atctgctgat tgtgctgtac cgcaatctgg tgatggttat gcgaatcgtc cgcatctacc
    15421 ggacacgtcc ggccttgtcc gagctgcttg gcattttttc ggatattgtc aatgtcatcg
    15481 cgactgtcaa ctatatcaat atgggcaaaa atctcattga gggactgggt tcacgggtgc
    15541 cgggcatcgg ccggtttgtt gatgacattg cacaaggcat cggagccggc tttatgacga
    15601 ctattaccgg ccatgctgcc atagaccgct gccggagttt tcgggagtgg aatgcggctg
    15661 aagccaaacg gcatctgctg agtcatgtgg gcgatttcta taacgatgtc aaggacatct
    15721 ttttcaagga tgtctggggc ggcatccgca gccggatcgg ggcagcgggg gataacaaag
    15781 acaaggttgc cgatgctctc aatgaaaccg gcagtctgct ggatagtttc atccgcgttc
    15841 cggctaaagc tgtcggagcc gccggtcagg ctgttgccgg aactactaac aaaggatttg
    15901 agttggtcgg cagaaaaatc ataaaagccg gcacagccct taccaagccg tttagaaaaa
    15961 caaagccgac cttgtaagac cctgccccgc atggagtttc aggagctggc gtcagtctga
    16021 aaatctcttt ttgtttctcg atttctaaac agataggctg taaagtgcag attttcctcc
    16081 agaggcattt tttattgggt tcggcgtgat attatcagac gatgcaaggg atgcagtttc
    16141 ttgatttttt aggcaaaatc cgctataatg aatatagtgt gcggaagcag atagtattca
    16201 attgtggggg tttgaaaaat gtttctatta cagatgttgg ctcgaagacg ccgtcatttg
    16261 agccgtaaac ctgcaagacg ggcaccgcgg tttctcaatc cgtccggcta tgcgctgaat
    16321 gcatggcgga ccttgtagtc cacatcggct gtttatcagg ccttttcgtt cagttgctgt
    16381 actgccggaa aggccttatt ttttgctctt cttttgctgc tgcagactcc ggcagtgtgg
    16441 tatcagcccc gggactttcc gcttcatttt ttcataacag catcaggttt ccgaatataa
    16501 aatggggtta aagacaaggg atcagcaaat cggtttaatg ccgccagccg ccggccaacc
    16561 cgatataacc ctccagcggt cgcaggccag aatgctttat ccagcagaca ggtaaaaggg
    16621 gacttgaatt tgtcagcgta gtagagcagt ccttccccga gcagtccgac atttttccgg
    16681 ccgctttgtg caagcctgtc cagcagttgt tcagcggtgg tcatttccgt accgattttt
    16741 ttttcccagc catttccggt ccagtcataa acagccgtat agaaaaaatc cttttttgca
    16801 tccagtacag tgacaatact gtctaacatc tggcctgtct gcaggatata gtctgtgccg
    16861 ttttctgcta acacatccat cgaatcagcg gcgataatcc ggctttttaa ggcaaaggcc
    16921 agcattttgg ccgccgtaac agcaatccgc aaaccggtaa aactgccggg gccgcctgtg
    16981 atatagacct gtcggatatc agaggggaca gcgcctgctt gtctcaataa ttcctcaatg
    17041 gcaccgaaca gttcagcact gtgccgcata aaacccgaaa agacgatttg ggctgcgagg
    17101 gtgtcatcgc gtccgatagc cgccgaaccg gttcgaccgg aggtttctat tgccagaata
    17161 agctgtgaat ctgtcattgg actcccgaca gttaaattgt ttgttgtggg cagaagatac
    17221 ccgtttttaa caataaaaac aagattgact aagtaagatt acggattttt tcgcttgcat
    17281 tcgtgatatt ttaactatac acttacgggt cgaccgatat aatctgtaaa tgcggtcttc
    17341 ttttggaatt atttattaac tctaatttcg gccttatttt atggaggctt taagtgaaga
    17401 cgctatattt ttggcgcagc tataagcatg cgggtattgc agcgatgctg gtattgtgcg
    17461 ggatgtggtg ttcgcttctt tgggctcagc cgtccaacat cgatcaggcc aagcgggcaa
    17521 aaattgacac cactatccga attgcccaag agcaaatgaa acgcggcttt tatcagcagg
    17581 cccaaacgca gctcaataca ttgctgactt cctctgaata caccaattac ctttcggaac
    17641 gacagcacca aacgattaca gcccttctga atgaaatctc tacagctctc caagaacgca
    17701 caaaaattac ccaaatgctt cagcaaagcg atacgcttgc gtctcaaggc aaatataccg
    17761 aggcgctggg gcttttgggg caaatcaaag acagtccgtt tgccagcgaa caagaacggc
    17821 agatgattcg ggacctctat caggatgttt ccgccaaaca gcttcaggag cagcagaaat
    17881 ggcagtcact gtatgatgcc agtactgccg cctataaggc cggccaatat gagcaggccc
    17941 gtcagggttt tgttcagatt cttgaagcag gggcatctgt caaaggcgac aaaacagctc
    18001 aagagtatat ccagctgatt gatagtgctg cagcgcagcc ggccggcacg ggtcaggcgg
    18061 actctgcaga gccgatggtg ccgctgctgg agacgccggc ggccgggacc tcatcggcgc
    18121 aggaggccat cgaaccgctg gatttgatgc cgcagccgga tgcatctgct caaactgtcg
    18181 caacggtatc ggttcaggta caggagcaga aggaacgatc gtatttggaa gtggttcgcc
    18241 agaaacgtgc tgttcaggtg gattatacga ctgctattgt aaaagatgcg attgaaaagg
    18301 ctcagcaggc gctggcgtcc aatcagtttg agcaggccca gcaggcactc cgccgggcat
    18361 cctccacggt tgaagccaat aaaatgctgc tgggagatgc gttttatgca gattacaaag
    18421 cgcagttaac caatctggag cagaagacag aggaggcccg tcaggcctat cagcagcagc
    18481 aggaaacgca gcgtcagcag caggccgatg agctgaatgc ccagattcgc caaacgatgg
    18541 aagagcagag ggcccgagcc gtcaaggatt atctggcacg ggcatatgcg ttcctgaaag
    18601 aacagcgcca tgaagatgcg ctagggcagc tggaacagct tttggcgatt gaccccctca
    18661 atcaggaggc ccagattctc aagcagacgc tggagcatat ggatcactat atcaagcagc
    18721 gtcagattca ggaagaaagc gacagggaag agattgccct gctgctggat gttaagcggc
    18781 gagcgattcc ttatgcggat gaattcaatt tcccccataa ctggaaggaa atttcggccc
    18841 gccgtgagca ggcggttaag gaggcccaga ctcccgaaga tatggctgtc aatgagctac
    18901 tcgataagac agtggacctt tcgatgctga cggaagatac aacattggcc gaggccatcg
    18961 aactgctgcg caattcggtg gatcctccgc ttccgattgt tgtgtactgg agcgatctga
    19021 gtcagaacgc ctttgtcgaa aaaacgacgc cgattaatat gagcggcgaa ggacttcggc
    19081 gtgtggtttt gcgcaccgca ttaagtcgag tacttcaggc ggttggttcc ggcggttttg
    19141 ccgaactggc ttatgttgtt caggaaggaa cagtgacggt tgccacccgc gattctctgc
    19201 ctgccaatta tgtaacagaa atttacgatg tagcagatct gctgaatcct ccggccaact
    19261 atgatgaaga ttacagcagc agcggctatg gcggcggtgg ctatggcggc ggtggctatg
    19321 gcggcggcgg ctacggcggc ggcggcgg
//
LOCUS       DAOTBO010000049        19264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2341_length_19264_cov_6.245822, whole genome shotgun sequence.
ACCESSION   DAOTBO010000049 DAOTBO010000000
VERSION     DAOTBO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 19264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19264
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_19264_cov_6.245822"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..508
                     /locus_tag="PLE88_07130"
     CDS             269..508
                     /locus_tag="PLE88_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76274.1"
                     /translation="MENNSAAARAARLKARGRKSPDERRRHISEQQPANSHQQTVIPN
                     GHEGSIISAIRLRRIPHFLVRRNLGEGGCYFIFDF"
     gene            complement(896..1264)
                     /locus_tag="PLE88_07135"
     CDS             complement(896..1264)
                     /locus_tag="PLE88_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76275.1"
                     /translation="MADGRTKEARDANAIGLISLNNKFNENEGEKDIREIDIIQDQRS
                     KNPEIEAQKRDLKNLISKGLTRAERLIIILYYYEEMTMKEIGTTLDLSESRVSQMHSS
                     IVARLKAQMTSRKKEFVINV"
     gene            complement(1280..1426)
                     /locus_tag="PLE88_07140"
                     /pseudo
     CDS             complement(1280..1426)
                     /locus_tag="PLE88_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1501..2559
                     /locus_tag="PLE88_07145"
     CDS             1501..2559
                     /locus_tag="PLE88_07145"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOM76276.1"
                     /translation="MRGVLYCMAFAQLTGRESLRDIEACLRAAGPKLYHAGMKTYVSR
                     TTLAKANEVRDWRIYRDIALILIEHARRLYTNQPLAMNLKRAIYAPDSTVIDLSLTLF
                     PWAQHRQHKSAVKIHTQLDLRGNIPAFIRITGGQIHDVHFLDHVLFEAGAFYIMDKGY
                     IDFQRLYHIHQQQSFFVTRAKDNLAFARHQSYPVDKNRGLRSDQAIRLTGPKSSVCYP
                     ELLRRIHYVDIELDKRFVFLTNHFNLSPWTIARLYKYRWQIELFFKWFKQHLHIKAFY
                     GTTPNTVKTQMWIAISVYVLVAILKEELDLEANMYEILQVLGMMLFEKTPIKSLFPAD
                     LHNFSKSELCKQLYLFDL"
     gene            3075..4478
                     /gene="dnaA"
                     /locus_tag="PLE88_07150"
     CDS             3075..4478
                     /gene="dnaA"
                     /locus_tag="PLE88_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOM76277.1"
                     /translation="MQAVITDEMQALHERIAAKIGTTQFQVWFKQAARLTLHSDHLEV
                     AAANPFICNWIQGHFAAAVSEALAEVIGQSRPLRFLIDNKLVKSSPRPILEQQKAERT
                     LNSLKRVPAVPKTAAGQLPLKLTMDTFVVGAKNQLAYNAAQSVIQEEKSPFNPLFLHG
                     GYGVGKTHLLQGVCNAIHVQRPGCRWMYLSAEDFANQYVVALKTRRLDEFRSRFRKLD
                     LLAIDDIHFLSNKNAMQEEFLHTFNSIDLAGKQIILASDAHPREIGQLCQKLVSRFIS
                     GMVVRMEAPDFEMRCRICSRRAQCMKLTLSQEVIEYIAGSITTNVRELEGALLKMAAF
                     ESLSGQPITLSAARQILAEHVVRMDPVVQNSEILSAAADLFGVSVSDMYSAKKDRTTT
                     LARSAAMYLTRRFTKMSFPEIGRAFGNKNHATVILACRKIDQALAAGKTFKWQTKGGW
                     QSMAAKEVLDALIEKIS"
     gene            complement(4542..5420)
                     /locus_tag="PLE88_07155"
     CDS             complement(4542..5420)
                     /locus_tag="PLE88_07155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOM76278.1"
                     /translation="MLLIPYAVDVPFDRRPFVNWLLTASIAAAFVWQVADKGQTVEQF
                     ALNGWTLKGLFGHLWLHADILHLIGNMIFLWVFGNAVCAKLGNLLYLPVYLLFGLAAA
                     TAHRFFDGSPAIGASGAINGVVGMFLVLFWQNEMDCLFVMFIFFRPIVKTFSISSFWM
                     IGLWLVFDILGAIVGGSNTAYFAHLGGFFMGIVLAVLLLKCNLVTMYSDEQSLVELVE
                     QWRQKLQDDKLERIARVSVNKAQKQDTAPPVPAMIRFVCPCGKQIKAPIQAAGKTGLC
                     PVCRRKLIVPHPTAEP"
     gene            complement(6332..6598)
                     /locus_tag="PLE88_07160"
     CDS             complement(6332..6598)
                     /locus_tag="PLE88_07160"
                     /inference="COORDINATES: protein motif:HMM:NF037947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage holin"
                     /protein_id="HOM76279.1"
                     /translation="MKLNVKAFGLACGLLWGIGVFFLTWWLIALEGIDAPPTIINRVY
                     PGYEITPIGAVVGMVWGFIDAGIGGAVFAWLYNLLALKFLNKCS"
     gene            6774..7250
                     /locus_tag="PLE88_07165"
     CDS             6774..7250
                     /locus_tag="PLE88_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOM76280.1"
                     /translation="MAKQISQERKSLYNAGLAVMIAGGCFFALPFLAVSALMAIMVIS
                     FGKSQAGIPIWMPIAGPIVMFVSFIGIALIVAGCVMRSIAARGTAGSGLVLNPEKARE
                     DLKPWTGMAGGMVKDILEEADIKSAGARQTVMIRCPHCQKLNEEDSKFCQECGKSL"
     gene            7491..9044
                     /locus_tag="PLE88_07170"
     CDS             7491..9044
                     /locus_tag="PLE88_07170"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOM76281.1"
                     /translation="MSAEKVIIFDTTLRDGEQSPGASLSVSEKLEIAQQLAKLGVDVI
                     EAGFPVSSPAQFEATRLCAEQVTGPVICGLARANAKDIEAAGRALTPAAKRRIHTFIA
                     TSPIHMEYKLRKKPAEVLKMAVEAVQYARQFTSDVEFSPEDACRSELTFLYEVLSAVI
                     EAGATTLNIPDTVGYMIPYEYGQLIANLKANVKGIDKAVISTHCHNDLGLATANSLAG
                     VRNGARQVECTINGLGERAGNAAMEEIVMAIHTRQDFFGSHLTTHINTREIFRTSQLV
                     SQLTGFVVQPNKAIVGANAFAHESGIHVDGILKKRETYEIMTPEMIGLSGSRMVLGRH
                     SGRHGFMDRCRQLGYNLSEKELEAAYEKFLEIADKKKEVFDEDIAAIINDEIRMVEQL
                     YHLEYLNVTSGTGTLPTGAVKIRLRDGQVRQAAACGDGPIDAAYEAIRQATGQTPTLD
                     NYSIRAITGGKEALGEAMVRIKDGKGRSFVGRGVSTDIIEASAKAYIDAINRMAAYSG
                     NNEAEKAGL"
     gene            9144..10403
                     /locus_tag="PLE88_07175"
     CDS             9144..10403
                     /locus_tag="PLE88_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOM76282.1"
                     /translation="MMTITEKILARAADLSSVKAGQLVNAKIDMIMCHDVTTPPAISM
                     LKQHGIDVVFDVNKIAVTPDHFQPAKDIKSAELHKRLDDWAREKKLPYYYKLGRAGVC
                     HALLPEQGHIRPGEVIVGGDSHTCTYGAFGAFSTGIGSTDLAAAIATGVLWFKVPASM
                     KFVLNGSLPDGVFSKDVILEVIRRIGTDGALYRAMEFTGPALKTMSMEARMTITNMAI
                     EAGGKNGIIGFDETTRAYLDGRLKGRMDYTIFESDADALYVSVETIDCSKLEPIVAFP
                     HLPSNGRMISDCKGLVMDQAYIGSCTNGRIEDLRIAAQILKGRQVKIRTIVVPATPVI
                     WKQAIDEGLAQIFYEAGCVISAPTCGACLGGFMGILAAGERCISTTNRNFVGRMGHPK
                     SEVYLASPATAAASAVEGRLADCRKYL"
     gene            10525..11016
                     /locus_tag="PLE88_07180"
     CDS             10525..11016
                     /locus_tag="PLE88_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOM76283.1"
                     /translation="MAKAHVYKRDHVNTDEIIPARYLNTDNPAELAAHCMEDLDTNFI
                     AKCRPGDVIVCGDDFGCGSSREHAVWAIQGAKVGAVIAHSFARIFYRNCINCGFYPIE
                     LPGALEQIHDGDELDIDYQAGVIDNKTQGRKIRFMPLPDFAIAIVNDGGLLNHIKKKQ
                     TLK"
     gene            11236..11481
                     /locus_tag="PLE88_07185"
     CDS             11236..11481
                     /locus_tag="PLE88_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76284.1"
                     /translation="MQAIPYLFSCSVESSLQGGSVVKAADVSKAQPLLPDQIFVSLVS
                     EAGTKKEVTVPASGRWIDYWNEQTVLTAGSTMQTPHM"
     gene            11601..12539
                     /locus_tag="PLE88_07190"
     CDS             11601..12539
                     /locus_tag="PLE88_07190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOM76285.1"
                     /translation="MKPKASKNRTIELTPQEFNSFSERLLTLHKPASRDEVLNKTIWQ
                     NMEQACRFLPPQIADLLFLDPPYNLNKNFNGRRFSKVPQEQYAKMLDGWLRLLTPLLK
                     PNASIYICSDWNACGVVQMTAGSYFKIRSRITWEREKGRGARTNWKNCAEDIWFCTMS
                     DDYTFHQEAVKLKRRVLAPYTDSAGQPKDWIPVAGGGFRLTAPSNLWTDLTVPFWSMP
                     ENTNHPTQKPEKLLAKIILASSSAGDIVLDPFCGSGTTSVVAKKLGRRYIGIEQDKFY
                     CCLTEKRLQMADCDKTIQGYYDGVFWERNTKPYPQR"
     gene            12655..16104
                     /locus_tag="PLE88_07195"
     CDS             12655..16104
                     /locus_tag="PLE88_07195"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOM76286.1"
                     /translation="MMGTIQFILGRSGTGKTHKCLEGVCSALEKEGEEPLVFLVPEQA
                     TYQAERAVLSQAHLRGFSRLRVLSFNRLAFWLVGRRAGIELSQTGRQMAVHKLLLDLS
                     DRLRVYKGHVYRTGLAGKLSELLCQLQAGSCTAAQIELLAQTLAQKAGQELAAAKWAD
                     IAALFSEYERFFQSSDRRFINPDADLAEAKKNAAGSFLRNCRLWVDGFSGFTGQEMEL
                     LAELLKICSEATIALCADPERLNLESDDESILDPCSVFAQTEQTYCQLRRMIRACRLA
                     IREPILLKKTYRFSQSPALEALEAALAAPDTAPKPIGREDAVHIAACSTVRSETLWIG
                     RTIRRLVKNGLRYRDIAVVIPNMTAYQHYIESAFGRYNIPYFLDRPRLMKSHPLVELL
                     GSALQAAVGGFGTSDVMSFLKSGMAGVPFDAVDDLENYCRAFGIEGEDWIRQTPWDFD
                     TESRFDQIGLDVLRQKAVGPLKALRAALQRCAKLTAAQFTQILWDFMETLDVRTSLSK
                     WAESDISDQQFGHRQLFSNLVSLLDEMNGVFGQTQLSAEDWSSIFMDALSGITIKLIP
                     PTLDQVLVGSIERSRHPEVQAVFLAGATQKQFPVPVMGQTLLTEQDYWLTEQAQLELT
                     NPYEQQLKYRPYLSYIALTRSRRQVYISYPLLDEKGSAVVPWSGLERLRALFPDLQIE
                     YPQAQAVKPEDIETPEQLGLWLAIHLGRDRREQRGSNQAVAAGVLDRVRNDEQAILQA
                     AGRQVSAALDYNNGACLPPDIADRLFAFPMKTSVTRLSNFAACPYQHFAKYVLGLEPR
                     KQLALEPADVGKFYHRVLERLFCALKRVGRDWANVPNEQLQDLCNQQIDEVLASDPQL
                     NNFTHRRIHHKYIIASAKETIGSFVFVLKQFAQASVFKQTEAELEFGPGCPLQVDLEI
                     QQGKRLLLSGRIDRLDIAEIDGKPAGVVYDYKKSGSKRADYAQILYGLDLQLPFYLLA
                     IRNYRPSKSASAEAGTGRTAAPAGAFYMPIDAGIDTKELSRLQRDAVNPKKAAGLFNG
                     CYFNAIDTGAVDKWSLYYSFFVDKGGAPYGYYDNSNALKPEDFTLLLDYTEQCIKRLG
                     TELCAGRIEIRPYRLGTHSPCGYCDYRPVCRFDWQINDYNSLDVCGKKEALEKMRKSL
                     EAV"
     gene            complement(16172..16816)
                     /locus_tag="PLE88_07200"
     CDS             complement(16172..16816)
                     /locus_tag="PLE88_07200"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOM76287.1"
                     /translation="MKWILNVMLVSIVLSGCQSTQTAEKGGWISLFDGKTLNGWTASE
                     NKDTFKVQDGMIVASGPRSHLFYTGPVQNADFTNFEWKADIMTLPNSNSGMYFHTQYQ
                     ETGWPDKGYEVQVNNSHSDWRRTGGLYAVSDVRQSPAKDNQWFTQHIIVRGKHIVVKV
                     NGITTVDYTEPDSIAQTGYPNMPGRKLSSGTFALQGHDPGSTVYFKNIMVKPLP"
     gene            complement(16907..18286)
                     /locus_tag="PLE88_07205"
     CDS             complement(16907..18286)
                     /locus_tag="PLE88_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOM76288.1"
                     /translation="MTDRNNLTARAAAWQALNQCDILRHDTAAILNRLLSRTDRPAQA
                     ADIVFGVIRSRGTLDYILKKYSKTDSSRVKTAAWNLLRIAVYELIYAPKTADYAIVNE
                     AVQLARDAESAKLAGFINAVLRNVQRAVESRLAPLQTESLRRMIPQTTQSGCLFADNL
                     LPDPVKETTQYFSTAFSLPQDLVNTWLKTYGPEQTRMLCFASNRHPSIIVQPNILLVT
                     AAELAQRLAADSVQTEMKAGVLRIRSTGRINTYPSYLEGLFYIQDTAAFDAIALAHPQ
                     SQWAALDLCAAPGGKCITLAMLTRDAGTILASDSSTQRLQMVRQNIQRLRLQSVKVIP
                     GGHIHAEVRRQKTLNLIILDVPCSNTGVLARRIEVRWRWKPRQIESLQQMQRRLLEQA
                     VQLAGPETKILYSTCSIQPEENQHQIAAFLAEHRRFALEAEKLTLPAVESEQAFDHDG
                     AYSALLRPK"
     gene            complement(18283..>19264)
                     /gene="fmt"
                     /locus_tag="PLE88_07210"
     CDS             complement(18283..>19264)
                     /gene="fmt"
                     /locus_tag="PLE88_07210"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOM76289.1"
                     /translation="LMKILYFGSAQFGIPCLEALLKSRHKLAGIFTQPARPAGRHRSQ
                     PTPTDVAVWCAAHGLPCIEADNINAAECISQVAGFGADLLVVIAFGQKVCQQIIGLHR
                     CGAINVHASLLPKYRGAAPIHWAIMNGEIETGVSIITLADRMDAGLILDQDRILIEPD
                     DTSQTLHDRLAQIAAPVLLRTIAKIEDHSIHPVEQDESQVTKAPRLRKEHGYIDWNKP
                     AAAIVNQIRALWPWPGAQSVYVSVKTGRHWRVTISRAQAVPAAGTSASVTGLLDDNLN
                     VVCGSEALKILELKPAGSDLMSFEAFVNGRPCGKGDLFVSSEKALSGLFR"
BASE COUNT         4886 a         4479 c         5158 g         4741 t
ORIGIN      
        1 ccgccattct ccctcgacaa tctttatttc ccccggcatt tcacatttgg catttcacat
       61 ttggcattta acatttggca tttcacattt ggcatttcac atttggcatt taacatttgg
      121 catctccgta ttcctgcatt cttgagttct tgggttctca ctccattgaa tcctaattcc
      181 gtcgagaaaa aaattctatt aatcccgtca aaataagttt gacaattaca aaacttatgt
      241 tataatacgc aaaaatagaa aatttagaat ggaaaacaac agcgcggcgg cgcgagccgc
      301 ccgattaaaa gcgcggggac gtaagtcccc cgatgaaaga aggagacata tcagtgagca
      361 gcaacccgcc aacagtcatc agcaaacggt catcccgaat ggacatgagg gttctattat
      421 ttcagccatc cgcctccggc ggatcccgca ttttcttgtc cgccgtaacc ttggtgaagg
      481 cggatgctat tttatctttg atttttgata tgattttacg aaacgaagcc aattttcaaa
      541 ggagaaaaac atccataagt cttttcaatc tcaagactaa ggtctcaggc ctcacggctg
      601 aggtggcaaa aacgaaccca atcgaaccca atctgaactt ctgccttctg gcttctgcct
      661 taaaaagaac aaacaaaccc aattttccat tttgcaaaat cgccataact cctttatttc
      721 taatgccttc tgcctattgc ctaacgcctg gcgcagccca aaacaaaccc aattcgaacc
      781 caaaagcaaa gccgattcga agccaaaacg aacccaatac aaacccgata gaaacccaac
      841 caaagccaat ttcaaaccag caaaatttct gcaacgacag ggggcttagc cgatttcaga
      901 cattgatgac aaattccttc ttccggctgg tcatttgggc tttgagccgg gccactatcg
      961 aggagtgcat ctgcgaaacc cgcgattcgg ataaatcgag ggtggttccg atctccttca
     1021 tcgtcatctc ttcatagtaa tacaggataa taatcagccg ctcagcccgc gttaagccct
     1081 ttgaaataag gttcttaaga tcgcgtttct gggcttcgat ttcgggattt ttgcttctct
     1141 ggtcctggat aatgtcaatc tcgcggatgt ccttttctcc ttcattttcg ttgaatttgt
     1201 tgttcagcga aatcagcccg attgcgtttg cgtcgcgggc ctcttttgtc ctgccgtcgg
     1261 ccatcgccct gggtaaaagt tcaagtgctt cctttttcca ttgaccaacc tggttgggat
     1321 ggacctcgta agccgaggcg atctcgttga cggtcttctg acctttgatg gcttcgatag
     1381 ctaccttggc cttgaatgcc gctgaaaaat gtctgcgttg tgccataatt ctctgctttc
     1441 ctttcatatc catacacgac agaattatac cacaaccacc accttatatc atggttcgaa
     1501 attcggggag tattatattg catggccttc gcccagttga ctggccgcga gagtttacgc
     1561 gatatcgaag cctgtcttcg tgccgcgggt ccaaaactgt atcatgccgg aatgaagacc
     1621 tatgtctctc gcaccacgtt ggccaaagcc aacgaagttc gcgactggag gatctatcgc
     1681 gatatcgccc tgattctgat cgaacacgcc cgcagacttt atacgaatca accactggcg
     1741 atgaatctca aacgagccat ttacgcaccg gattctacgg tcatcgattt atccctgacg
     1801 ctgtttccat gggctcagca tcggcaacac aaaagtgccg tcaagataca tactcaacta
     1861 gacctacgag gcaatattcc ggcgtttatc cgcattacag gcggccagat acacgatgta
     1921 cattttctcg accacgtgct attcgaggcc ggagccttct atattatgga caaggggtac
     1981 atcgattttc agcgtttgta ccatatccat cagcagcaat cgttctttgt cacgcgtgcc
     2041 aaagacaacc tggcttttgc acgccatcaa tcctatccgg tggacaaaaa tcgggggctg
     2101 cgcagcgatc aagccatccg tttgacgggt ccgaaaagct cggtatgtta tcccgaactg
     2161 ctacggcgaa tccattacgt cgatatcgaa ctggacaagc gtttcgtgtt cttgaccaac
     2221 cacttcaatc tgtcgccgtg gacgattgct cggctgtaca aatatcgctg gcagatcgaa
     2281 ttgttcttca aatggttcaa gcagcatctc cacatcaaag cgttttatgg cacgacaccc
     2341 aacaccgtga agacgcagat gtggatcgcc atcagtgttt atgtgctggt tgcgatccta
     2401 aaagaggaac tcgacctgga agccaatatg tacgaaattc tgcaagtttt gggcatgatg
     2461 cttttcgaga aaacacccat aaaaagcctg tttcctgcgg atcttcacaa cttttcaaag
     2521 agcgagttat gtaagcaatt atatctattc gacttataac gggacagtag tgatttgaca
     2581 tcataaaatt gatgccattt atcaagtctg attttccagc actagacata ctgttagaaa
     2641 gtgccctacc gacgggatat tgttgataaa tataaaaatt taacagcatt ccccaagcag
     2701 gttcagtaca ctttgatctt tcggtccttc ctatccgcca aacacagctg ctaattctga
     2761 aaattcgtta tttatacgac ccgatgcctg tgagtttttt cccttatgat aagtaggatc
     2821 ttgactgctt gccaaaatta gaaccttcat tcacagctta ttcacatagc ggatttaggt
     2881 tgtgaaaaaa ttgttcaaaa gaaaattttt ttgcatataa gttatgtctg cactgaaatc
     2941 ttttctctgt ggaaaagtat tttcaaaaaa caccattgca accttttgtc ttgctaccta
     3001 tgatttgcct aaccggcgct ggccgggtag tcgatttcaa cattgaaaat actgcggtgg
     3061 atgggagtta aactatgcag gctgttatca cggatgagat gcaagcgctt catgaaagga
     3121 ttgctgccaa aatcggaact actcagtttc aggtttggtt caaacaggct gcgcgcctga
     3181 cgcttcacag cgatcatctg gaagtggctg ccgccaatcc ttttatctgc aattggatac
     3241 aggggcattt tgctgccgct gtttcagaag ccctggcaga ggtcattggt cagtcgcgcc
     3301 cccttcgctt tctgatagac aataaattag tcaaatcctc gccgcgtcca atccttgagc
     3361 agcagaaggc cgaacgaaca ctcaattctc ttaaacgggt gcctgctgtg ccaaaaacag
     3421 cagccggtca gcttcctctc aagctaacaa tggatacttt tgttgttggt gccaaaaatc
     3481 agctggctta taatgccgcc cagtccgtta ttcaggagga aaaaagtcca ttcaatccgc
     3541 tattcctcca cggcggctac ggcgtgggca agacacattt gcttcagggt gtctgcaatg
     3601 ccatccatgt ccagcgcccc ggctgccgat ggatgtattt gtcagcggaa gattttgcga
     3661 atcaatatgt tgttgcgctg aaaacacgga gattggacga atttcgcagc cggtttcgca
     3721 agctcgattt gctggcgatt gatgatatcc attttttatc gaataaaaat gccatgcagg
     3781 aagaattcct gcataccttt aacagcatcg atttggccgg aaaacagatt attctggcat
     3841 cggatgcaca cccaagagaa atcggacagc tttgccaaaa gcttgtcagc cgctttattt
     3901 cgggaatggt ggtgcggatg gaggcgccgg attttgaaat gcgctgccga atttgctccc
     3961 ggcgggcaca gtgcatgaag ctgaccctgt cacaggaggt gatcgaatat atcgccggca
     4021 gtatcactac caatgtacgg gaactcgaag gtgccctgct gaagatggcg gccttcgaat
     4081 ccctcagcgg acagccgatt accttgtcgg cagcccgcca gatactggcc gaacatgtcg
     4141 tccggatgga tccggtcgta caaaattccg agattctctc ggcagcggcc gatttgttcg
     4201 gagtcagcgt ttcggatatg tattcggcga aaaaagaccg caccaccacc ctggctcgtt
     4261 ctgcggcgat gtatttgacc cgtcggttta ccaaaatgag cttcccggaa atcggacggg
     4321 cattcggcaa caagaaccat gctaccgtta ttttggcatg ccgcaaaatc gaccaagctc
     4381 ttgctgcagg aaaaaccttc aaatggcaga caaaaggcgg ctggcaaagt atggcggcta
     4441 aggaagtcct tgatgccctt atcgagaaaa tctcctgacg tggaacgatg gccggaagca
     4501 ggcaagggat taagcagcat aaaggcgaaa atcagcccat cttaaggctc agcggtcggg
     4561 tgtggtacaa tcagttttcg cctgcagacc ggacataaac ctgtcttgcc tgctgcctga
     4621 atcggcgcct taatctgttt gccgcaggga caaacaaatc gtatcatagc cggcaccgga
     4681 ggcgctgtgt cctgtttttg ggccttattg acagaaaccc gggctattcg ctccaatttg
     4741 tcatcttgga gtttttgacg ccattgctcc accagttcca ccagcgactg ctcatcgctg
     4801 tacatcgtaa caagattgca cttcaggagc aaaacggcca gaacaatccc cataaaaaag
     4861 ccacccagat gtgcaaaata ggctgtattg ctgccgccta ctatagcccc aagaatatca
     4921 aacaccagcc acagtccaat catccagaag ctggaaatac tgaacgtttt gacaatcggc
     4981 ctgaaaaaga taaacataac aaacaggcag tccatttcgt tttgccaaaa caaaacaaga
     5041 aacattccca cgacaccatt gatagccccg ctggctccta ttgccggact gccgtcaaaa
     5101 aaccgatgag ccgtcgctgc agccagtcca aacagaagat aaacaggcag ataaagcaaa
     5161 ttgcctaatt tggcgcaaac tgcattgcca aacacccaaa gaaaaatcat attgccaatc
     5221 aggtgtaaaa tgtcagcatg cagccaaaga tgaccgaaca aacctttcag cgtccagccg
     5281 ttgagtgcaa actgctcgac cgtttgtcct ttatctgcga cctgccagac aaatgctgct
     5341 gctatcgaag ctgtcagtag ccaattaaca aatggacggc ggtcaaaggg aacatcaacc
     5401 gcatagggaa tcaacagcat cttagcctcc tgataagtct aataaaatca taaccttaaa
     5461 gatatgacgg gtgcaagcac ttttcagtga aaataggcgg atatattcgg tctaaaatgt
     5521 gtcaatgtat aaaagaaaag cagcgggaag gaggaacccg ctgctctgtt ccaggtagaa
     5581 tacctggcat tagaaggagg aggagggtaa tgtctttttt tatctttctg tctatacgaa
     5641 agaaatctta cattgtttta gtaaattata catcatatcg ggaaaaaatc aagaaaaatt
     5701 cagtaaaaat acctaaaaaa agcgacccga aagaaatcgg gtcgcttttt gggcaggtat
     5761 taaggtgtcc ataccactgg aggaagaaag atgaaaaaga gtacttactc atactacgta
     5821 ataggtaagg catggtttgc cataaatgct ctaattattt tgtacttctt gaaataaggg
     5881 ataaaattaa tggcttgtaa cttttctaaa ccattatatg aaatatagat atgtatgttt
     5941 gggcgagttt tttcttgaga aataagagat aattggatat aataataatt gatgagtatg
     6001 gcttcggtca tggggggtta taaagaacca aaacttgggg agaaaacgat gaaaaaatat
     6061 tattacaggt tctgggttcc gtttgtcgcg aataaaaagc gaattcttat tcttgcaatc
     6121 gccagctata tagcgatgtg ctgatttttc ctgcaaaacc accatcctga taagggattg
     6181 cataaaaaac gccagctacg ccaacagccg gcgttttttg ttaagtttct gctggaaacc
     6241 gttaaaaata cacagctata cacacattca ttgctaaaaa atcaagcgat gctgcaatcg
     6301 aatcctttct aacagggtca agaaaagctc tttatgagca tttatttagg aacttaaggg
     6361 ccagcaggtt atacagccaa gcgaaaacgg ctcctccaat gcctgcgtcg ataaagcccc
     6421 agaccattcc gactactgca ccgataggtg ttatttcgta gccgggatat acgcgattaa
     6481 taatggttgg cggggcatcg ataccctcca aagcgatcag ccaccaagtc agaaagaaga
     6541 cgcctattcc ccacaataaa ccacaggcca gcccaaaggc cttcacgttc agcttcataa
     6601 tcgctactcc ttcaaaaaac gatggttata aagtttttta ctttatgtgt atattgataa
     6661 caataaaggc aagcgattta caagaaaaaa cggcacagga agcggaaata gttgtatatt
     6721 gaaatatgcg ggagtagtat agtggtgtaa tatattatta ttgggagttt gttatggcca
     6781 agcagatttc gcaggagcgt aaatcgcttt ataatgccgg cttagctgta atgattgcag
     6841 gagggtgttt ttttgcactg ccttttttgg ccgtttcggc tcttatggcg attatggtga
     6901 tcagttttgg caagagccaa gccggtattc ccatatggat gccgattgcg gggccgatag
     6961 tgatgtttgt gtcgtttatc ggcattgcgc tgattgtggc tggctgtgtg atgcggtcta
     7021 tagccgcgcg cgggacggca gggtcgggac ttgtgctgaa cccggagaag gcacgtgaag
     7081 atcttaagcc ttggactgga atggccggcg gaatggttaa ggatatatta gaagaggcgg
     7141 atattaaatc tgccggcgcg cgacagacag ttatgattcg ctgtccgcac tgccaaaaat
     7201 tgaacgaaga ggactcgaaa ttttgtcaag aatgtgggaa atccctataa tttaagtttt
     7261 ttcttgcttt cgagccgcta aaaagagtaa aattacaaac gtatggagca taaaaaagat
     7321 cagaatgttt ttgtttctgc cgttatcgcc ggtctgatcc ttctgcgccc ctcgggcgca
     7381 taaacgacat tacacatctt aaacccatcg tattttcaaa ttccagtgga tttttacgct
     7441 tattttatgc ataataggcg gcttattctg actatcagga gacctataca atgtcagcgg
     7501 aaaaggtgat aatttttgat acgactctgc gtgatgggga gcaatcgccg ggggcgtcgc
     7561 tttcggtcag cgaaaaactc gaaatagccc agcaattggc aaaactgggc gttgatgtta
     7621 ttgaggcggg atttccggtg tcttcaccgg cccagtttga ggccactcgg ttgtgtgcag
     7681 aacaggtgac cgggccggtg atctgcggac tggcgcgtgc gaatgccaaa gatatagaag
     7741 ccgccggcag ggcactgacc ccggcagcca agcggcgaat tcatacattt atcgccacca
     7801 gcccaattca tatggagtac aagctccgca aaaagccggc agaggtgctc aagatggctg
     7861 tggaggcagt gcagtatgca cggcagttta ccagcgatgt cgagttttcg ccggaggatg
     7921 cctgccgcag cgagttgacg tttttatatg aggttttgtc ggcggttatc gaggccggcg
     7981 ctacgacact gaatatcccg gacacggtgg gttatatgat accctatgag tacggacagc
     8041 tgatagccaa tttgaaggca aatgtcaagg gtatcgataa ggcggtcatc agtacgcact
     8101 gccataatga tttgggcttg gcgacagcca attccttggc aggagtgcgc aacggtgccc
     8161 gtcaggtgga atgcacaatt aacggactgg gcgagcgtgc gggcaatgcg gcgatggaag
     8221 aaattgtgat ggcaattcac acccggcagg atttctttgg cagtcatctg acgacgcata
     8281 tcaatacccg tgaaatattc cgcaccagcc agctggtatc ccagctgacc ggttttgttg
     8341 tgcagcccaa caaggcaatc gtcggcgcca atgcctttgc gcatgaatca gggattcatg
     8401 tggacggcat tttaaagaag cgcgagacgt atgaaattat gacgccggag atgattggct
     8461 tgagcggttc gcgaatggtg ctggggcggc attcaggtcg gcacggcttt atggatcgct
     8521 gccggcagct gggctacaac ctcagcgaaa aggagctgga agcggcgtat gagaaatttc
     8581 tggaaattgc cgacaagaag aaagaggtat ttgacgagga tattgccgct atcatcaatg
     8641 atgagattcg gatggttgag cagctttacc atctggaata cctcaatgtt accagcggca
     8701 caggaacgct gccgactggt gcggtcaaaa ttcggctgcg ggatggacaa gtgcgtcagg
     8761 cggccgcctg cggcgatgga ccgattgatg cggcctacga ggcaattcgt caggccacag
     8821 gccagacgcc gacactggat aattactcga ttcgggcgat taccggcggc aaggaagcat
     8881 tgggtgaggc aatggttcgc atcaaggacg gcaagggtcg gtcgtttgtg gggcggggtg
     8941 tttcgacaga tatcattgaa gccagcgcga aagcttatat tgatgcgata aaccggatgg
     9001 cggcttattc aggaaacaac gaggccgaaa aggcgggctt atagagaaag actgctgcgg
     9061 caatgaaatg gcattttgcc ggtgcagttg tttacaggat taaatgctga tttgggacaa
     9121 gatttgattt caggaagcaa gcgatgatga caataaccga aaagatactg gcgcgtgcgg
     9181 cagatctgtc gagcgttaag gcaggtcagc tggttaatgc gaaaatcgat atgattatgt
     9241 gccatgatgt aacaacgccg ccggcaatat caatgctgaa acaacatggg attgacgtgg
     9301 tattcgatgt gaacaaaata gcggttacac cggatcattt tcagccggcc aaggatatca
     9361 agagtgccga acttcacaag cggctggatg actgggcgcg ggaaaagaag cttccgtact
     9421 attacaaact cgggcgggca ggcgtatgtc atgcgctgct gccggagcag ggacatatcc
     9481 ggcccggcga ggtgattgtc gggggagatt cgcatacctg cacctacggc gcgtttgggg
     9541 cgttttcaac gggcatcggc tcgacagatt tggcggcggc aattgccacc ggtgttctgt
     9601 ggtttaaggt gccggcgtcg atgaagtttg tactgaacgg ttcgctgccg gatggggtat
     9661 tcagcaagga tgtgattctg gaggttattc ggcgcatcgg gaccgacgga gcgctgtaca
     9721 gggcgatgga gtttacgggg ccggcgctga aaacgatgtc aatggaagcg cgaatgacga
     9781 tcaccaatat ggctatcgaa gccggcggca aaaacggcat catcggcttt gatgaaacaa
     9841 cccgggcgta tctggacggt cggctcaaag gccggatgga ttatacgatt tttgaatcgg
     9901 atgcagatgc cctgtacgtc agtgtggaga caatagactg ctcaaaatta gagccgatag
     9961 ttgcatttcc ccatcttcca agcaacggcc ggatgatcag tgattgcaaa gggctggtga
    10021 tggaccaagc ttatattgga agctgcacca acgggcggat cgaagatctg cggattgcag
    10081 cgcagatact caaaggccga caggtcaaaa tccgcacaat tgttgtgccc gctacgccgg
    10141 ttatttggaa gcaggcaatt gacgaaggcc tggcccagat tttttacgag gccggctgcg
    10201 ttatcagtgc gccgacctgc ggggcgtgtc tggggggctt tatgggcatt ctggcagccg
    10261 gcgaacggtg catcagcacc accaaccgca attttgtcgg ccggatggga catcccaaga
    10321 gcgaggtata tctggccagt ccggcaacgg cggctgcatc ggcggttgag ggacggttag
    10381 ccgactgcag gaaatatctg tagccggcag ccgaagttga acacggtttt gagtgcatca
    10441 gagaaaaaat gtgcgactag tagtatttga ttgtttaatg cttgctgtca ggtcagcggc
    10501 attcaggatt tttgggagaa tggtatggca aaagcacatg tatataagcg tgaccacgtg
    10561 aacacggacg aaataattcc cgcccggtat ttgaacaccg ataatccggc ggaattggcg
    10621 gctcactgca tggaggactt ggatacaaat tttattgcca agtgccggcc gggtgatgtg
    10681 attgtctgcg gggatgattt cggctgcggc tccagccgcg aacacgctgt ctgggcaatt
    10741 cagggagcaa aagtcggggc cgtgatagcc cattcctttg cccgcatttt ttaccgcaac
    10801 tgcatcaatt gcggatttta cccgatagag ctgccgggcg ccctggagca aatccatgac
    10861 ggcgacgaac tggatattga ctatcaggcc ggcgttattg ataacaaaac gcagggcaga
    10921 aaaatcaggt ttatgccgct gccggatttt gcgattgcga ttgtcaacga cggcggcctg
    10981 ctgaaccata ttaaaaaaaa gcaaactctc aagtaggcag cccgctgcct gtagtgctgt
    11041 cggcaggatc agaagagatt gatttagtca gccaatcggg atgtcccagc agaactgatt
    11101 caagcgattg aggagactgc ataatgaaca gatgcattgc tgttgaactg ctgctggctc
    11161 cctttgcggg cttagcggcg ggcgggtgga tttgttcgac ggcgaaatct tcgcagcatc
    11221 cgattaagga gccgtttgca ggcaatcccc tatctgttca gctgttcggt ggaatccagc
    11281 ctccaaggcg gcagcgttgt caaagcggcc gatgtttcca aagcccagcc tctgctgcca
    11341 gaccagatat ttgtatcgct ggtttctgaa gctggaacga agaaagaggt cactgttcct
    11401 gcaagcggac gatggattga ctattggaat gaacaaactg tgctgacggc tggaagtacg
    11461 atgcagacac cgcatatgtg ataggcgaca aggcaggcat cgattttaaa attcaaatcg
    11521 atggtctgac aggttagtcg ggactggccc agctgtaatt tgtgctgcgc cggatccatt
    11581 gctgtgggga tttttatcat atgaagccaa aagcttcaaa aaaccgtacg attgagctga
    11641 cgccgcagga atttaattcg ttttcagagc gtttactgac gctgcataag ccggcgtcac
    11701 gcgatgaggt gctgaataag acgatttggc agaatatgga gcaagcgtgc cgctttctgc
    11761 ccccgcagat tgcagacctg ctgtttctgg acccgcctta caatctcaac aagaacttta
    11821 acggcagacg attttcgaaa gtgccgcagg aacagtatgc aaagatgctg gacggatggc
    11881 tgcggttatt gacccctctt ctgaagccca acgcttctat ctatatctgt tcagactgga
    11941 atgcctgcgg ggttgtgcag atgacagcgg gcagctattt caaaatccgc agccggatta
    12001 catgggaacg agagaaggga cgcggggcca ggaccaactg gaaaaattgt gctgaagata
    12061 tctggttttg tactatgtct gacgattata cgtttcatca agaagcggtc aaattgaagc
    12121 ggcgggtttt ggctccttat acggattctg ccggtcagcc gaaggattgg atacctgttg
    12181 caggtggagg gtttcgcctg acagccccgt ctaatctgtg gacggatcta acggtaccgt
    12241 tttggtcgat gcccgaaaat accaatcacc ccacacagaa accagagaaa ctgctggcca
    12301 agattattct ggcaagctct agtgcagggg atatagtgct ggatcctttc tgcggctccg
    12361 gcacaaccag tgttgttgcc aaaaaactgg gccgccgata tatcggcatt gaacaggaca
    12421 agttttactg ctgcctgacc gaaaaacggc tgcagatggc tgattgtgac aaaacgattc
    12481 aaggttatta cgacggtgtt ttttgggaga gaaacaccaa gccgtatcct cagcggtgaa
    12541 gaaggagtat cccgataatc caacagcggc cgaaaagggg ggagtttatg attgcaggca
    12601 gagggtacat atggtaaaaa gagagtggta tgttacacct gcaaaggaag gaatatgatg
    12661 ggaaccatcc agtttattct cggccgaagc ggaaccggaa agacgcacaa atgtctggaa
    12721 ggcgtttgtt ctgcgctgga aaaagaaggg gaggagccgc tggtatttct ggtgccggaa
    12781 caggcgacgt atcaggcaga acgggctgtt ttgtcgcagg cgcatctcag gggcttcagc
    12841 cgtttacggg tgctgtcatt taaccggctg gctttctggc tggtcggccg ccgagcggga
    12901 atagaattgt cgcaaaccgg ccggcaaatg gcagtgcata agctgctgct ggatttatcc
    12961 gaccggctgc gggtgtataa gggccatgta tatcggacgg gattggccgg caagctttcc
    13021 gaattgctct gtcagcttca ggcaggcagc tgcaccgccg cccagattga actgctggct
    13081 caaacactgg ctcagaaagc cggtcaggaa ctggctgctg ccaagtgggc ggatattgcg
    13141 gcactgtttt ccgaatatga acgttttttt caatcttctg atagacggtt tatcaatccg
    13201 gatgcggatt tggcggaagc caaaaagaat gcggccggct cttttctgcg aaactgccgt
    13261 ctgtgggtgg atggtttttc tggttttaca ggccaggaga tggaattgct ggcagagttg
    13321 ctgaagatct gcagcgaggc gacgatagcc ttatgtgcag atccggagcg gctgaatttg
    13381 gaaagtgacg acgaaagcat ccttgatccg tgcagcgtgt ttgcccagac tgagcagacg
    13441 tattgccaat tgcggcgaat gatacgggcc tgcaggctgg caatccgaga accaatactt
    13501 cttaaaaaga cgtaccgttt cagccaatcc ccggcattgg aagcactgga agcggctctt
    13561 gcggctccgg atacagcacc gaagccgatc ggcagagagg atgcagttca tattgccgca
    13621 tgcagcacgg tgcgcagcga gacactctgg ataggccgga cgattcggcg gctggtcaaa
    13681 aacggtcttc gctatcggga tatagccgtg gtaataccga atatgactgc gtatcagcat
    13741 tatatcgagt cggcattcgg ccggtataat attccgtact ttttggacag gccgcggctg
    13801 atgaaaagtc acccgttggt cgaactgctg ggttcggctc tgcaggcagc cgtggggggc
    13861 tttggcacgt cagatgtgat gagttttctt aaatctggaa tggccggcgt gccgtttgat
    13921 gcggtcgatg atttggaaaa ctactgtcgg gctttcggca ttgagggcga ggattggatt
    13981 cggcagacac cgtgggattt tgatacggaa agccgatttg accaaattgg tctcgatgtg
    14041 cttcggcaaa aggcagtggg gccgctgaaa gctttgcgtg cagcgctgca gcggtgtgcg
    14101 aaactaactg cggcacagtt tacccagata ctttgggatt tcatggaaac gctggatgtg
    14161 cggacatcgc tgtcaaagtg ggcagaaagt gatatttccg accagcagtt cggacatcgc
    14221 cagttatttt ccaatctggt cagtttgttg gatgagatga acggtgtgtt tggccagaca
    14281 cagctgtcgg cggaagattg gagctctatt tttatggatg cgctctcggg gataactatt
    14341 aagctaattc cgccgactct tgatcaagtc ttggtcggat cgattgaacg cagccgtcat
    14401 cctgaagtgc aggcggtttt tctggcaggg gcaacgcaga agcagtttcc tgtgccggtt
    14461 atgggacaga cactgctgac cgagcaggat tattggctga ccgagcaggc ccagctggaa
    14521 ctgaccaatc cgtatgaaca gcagctcaaa tataggcctt atttgtcgta tattgctctg
    14581 acgcggtcac gccggcaggt gtatatttcc tatccgcttc tggacgagaa gggttcggct
    14641 gttgtgccgt ggtctggatt ggagcggctg cgggcattgt ttccggactt gcagatagaa
    14701 tatccgcaag ctcaggccgt caaaccggag gatattgaga ctcctgaaca gctgggtctc
    14761 tggctggcaa tacatctggg tcgcgaccgc agagagcagc ggggcagcaa tcaagcagta
    14821 gctgcggggg tgctggacag agttagaaac gatgagcagg cgatcttgca ggcagcaggc
    14881 cggcaggttt cagcagcact ggactataac aacggtgcct gtcttccgcc ggatatagcg
    14941 gaccggcttt ttgcgtttcc gatgaagact tctgtaacgc ggttgagcaa ttttgcggcc
    15001 tgtccgtacc agcattttgc caagtatgtg cttgggctgg agcccagaaa gcagcttgca
    15061 ctggaaccgg ctgatgtggg gaaattttat catcgagtgc tggaaagatt gttttgtgca
    15121 ttaaaacggg tcggcaggga ttgggcaaat gtgccgaatg aacagctgca agacctgtgc
    15181 aatcagcaga tagatgaagt tcttgcttct gatccgcagc tgaataattt tacacatcgc
    15241 cggatccatc acaaatatat tattgcgtca gccaaagaga ctatcggcag ttttgttttt
    15301 gtcttaaaac aatttgctca ggccagtgtt ttcaagcaga cagaggcgga gcttgaattc
    15361 gggccgggct gtccgctgca ggtggatttg gaaattcagc aaggcaaacg actactgctg
    15421 tccggacgaa tcgaccgtct ggatatcgct gaaattgacg gcaaaccagc cggtgttgtg
    15481 tatgactata aaaagagcgg cagcaaacgg gcggattatg ctcaaattct ttatggattg
    15541 gatttacagc ttccgtttta tttgctggct atccgtaatt accggccgtc aaagtcggcg
    15601 tcggctgaag caggaacagg ccgaacagct gctccagcgg gggcatttta tatgccgatt
    15661 gacgcaggaa tagatacaaa agaactttcc cggctacagc gggatgcagt gaaccccaag
    15721 aaggctgctg ggctcttcaa cggatgctat tttaatgcta tcgacacggg tgcggttgac
    15781 aaatggagcc tatattacag tttttttgtg gacaagggtg gggcacctta cggctactat
    15841 gataacagca atgcattgaa gccggaggat tttaccctgc tgctggatta cacggaacaa
    15901 tgcatcaaaa gattggggac cgagctttgt gcaggcagaa tcgagattag accctatcgt
    15961 ctgggcacac actcaccgtg cggttattgt gattatcggc cggtctgccg atttgattgg
    16021 caaatcaatg actacaacag tctcgatgtc tgcggcaaaa aagaggcatt ggagaaaatg
    16081 cgaaagtccc tagaagcagt atagcgggat aaaaacgaag gcctcgcatt gacagcgagg
    16141 ccttcgaaaa aggcgggctt gctggtgttc atcagggcag aggcttgacc ataatgtttt
    16201 tgaagtacac ggtgctgccg gggtcgtgtc cctgaagggc aaaggtgccg ctggacagtt
    16261 tgcgtccggg catattggga tagccggtct gggcgatgct gtcgggctcg gtatagtcta
    16321 ccgttgttat accgttaact ttgacaacga tgtgcttgcc gcggacaata atatgctggg
    16381 tgaaccattg gttgtctttg gccggcgact ggcgtacatc gctaacggca tacagtcctc
    16441 cggttcgccg ccagtcgctg tggctgttat tgacctgcac ttcatagccc ttatccggcc
    16501 acccggtttc ctgatactga gtgtgaaaat acattccgga attggaattg ggcagggtca
    16561 ttatatcagc tttccattcg aaattggtaa agtcggcgtt ttgcacgggg ccggtgtaga
    16621 acaaatggct gcgaggaccg ctggcaacaa tcataccatc ctgcactttg aaggtgtctt
    16681 tgttttcgct ggctgtccag ccgttcaggg ttttaccatc gaacagactg atccaacccc
    16741 ccttttcagc tgtttgtgtg gactgacagc cgctcagcac gatactgaca agcattacat
    16801 tcagaatcca tttcattttt tgtctccctt acttgttttt actttttaca taactgcagg
    16861 attgtcataa ttattaaaca ggtagactat gattttcgga ataggtttat ttaggcctca
    16921 gcaaagccga ataagcccca tcatgatcaa aggcctgctc gctttcgacc gccggcaggg
    16981 ttagtttttc tgcctcgagt gcaaagcggc ggtgttcggc cagaaaagcg gctatttggt
    17041 gctgattttc ttccggctgg atgctgcagg tggaataaag gatttttgtt tcgggacctg
    17101 ccagctgaac ggcttgctcc agaagccggc gctgcatctg ctgcaaagac tctatctgac
    17161 ggggcttcca tcgccagcgg acttcgatac ggcgtgccag tacaccggta ttagagcagg
    17221 gaacatccag tataataaga ttcagcgttt tctgtctgcg tacctctgca tggatgtgtc
    17281 cgccgggtat cactttgacc gactgcagcc gaagccgctg gatattctga cgcaccatct
    17341 ggagacgctg agtactgctg tcgctggcca gaatggtgcc ggcatctctg gtcagcatag
    17401 ccagcgtaat gcatttgccg ccgggggcag cgcacaaatc cagcgctgcc cactgggact
    17461 gcggatgagc caaagcgata gcatcgaaag cagctgtatc ctgaatataa aaaagccctt
    17521 caagatagct gggataggta ttgattctgc cggtgctgcg gatgcgcagg accccggctt
    17581 tcatctctgt ctggacagaa tcagcagcca gccgctgcgc cagttcggcg gctgtaacaa
    17641 gcaggatgtt gggctgaaca ataatcgaag gatgacggtt ggaggcaaag cacagcatac
    17701 gggtttgttc tggaccgtag gttttaagcc acgtgttgac cagatcctgc ggcagagaaa
    17761 aggcggtgct gaaatattgg gtggtttctt tgacgggatc gggcagcagg ttgtcggcaa
    17821 agaggcatcc gctttgggtt gtctggggaa tcatccggcg aagcgactcg gtctggagag
    17881 gcgcgaggcg cgattccaca gcccgctgga cattgcgcag cacagcattg ataaaaccgg
    17941 ccagtttggc tgattcggca tctcgggcaa gctggacagc ttcattgaca attgcatagt
    18001 cggcggtttt gggggcataa atcaattcat agactgctat ccgcaaaaga ttccatgcgg
    18061 ctgtttttac ccgggaggaa tcggtttttg agtatttttt aaggatgtaa tcaagcgtac
    18121 cgcggctgcg aataacgccg aacacaatat cggctgcctg tgccggacgg tcggttcggg
    18181 atagcagacg attgaggatg gcggctgtat catgacggag tatatcgcac tggttaagtg
    18241 cttgccaggc ggcggcacgg gctgtcaggt tgtttctatc tgtcatcgga ataatccgct
    18301 tagagccttc tctgaagaga caaacaggtc tcctttcccg caggggcgtc cgttaacaaa
    18361 cgcttcaaat gacatcaaat cagaaccggc gggcttgagt tcgagtatct tcagcgcttc
    18421 gctgccgcag acgacattga gattgtcatc cagcagtcct gttacggatg cggatgtgcc
    18481 ggcggcgggc acggcctgcg cccggctgat cgtaacgcgc caatgccggc cggtcttgac
    18541 cgatacatac acgctttgag cgcccggcca aggccagaga gcacgaattt ggttgacgat
    18601 agcagcagcc ggcttgttcc agtcgatata tccgtgctct tttcgcagac gcggggcttt
    18661 ggtgacctgg gattcatcct gctcaaccgg atgaattgag tggtcttcta tttttgcgat
    18721 ggtcctcagc agcacgggag cggcaatttg agccagacgg tcatggaggg tttggctggt
    18781 atcgtccggc tctataagga tgcggtcctg gtccagaatc aatccggcat ccatacgatc
    18841 ggccaaagtg attatgctga cgcccgtttc aatttcaccg ttcattatgg cccagtgaat
    18901 cggtgcagcc ccgcggtact tgggcagcag tgaggcatgc acgttgattg caccgcaccg
    18961 gtgaagtccg atgatttgct gacagacttt ctgtccgaag gcaatgacaa ccagaaggtc
    19021 agccccaaag ccggcgactt ggctgatgca ttcggcagca ttgatattat ccgcttcaat
    19081 gcagggcagg ccgtgggcgg cacaccatac ggcaacatcg gtcggtgtcg gctggctgcg
    19141 atggcgcccg gcaggacgcg ccggctgagt aaagatgcct gccagcttgt gacggctttt
    19201 taaaagagcc tcgaggcaag ggatgccgaa ttgggcagaa ccaaagtaga gaatcttcat
    19261 aagg
//
LOCUS       DAOTBO010000050        18918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2405_length_18918_cov_5.594762, whole genome shotgun sequence.
ACCESSION   DAOTBO010000050 DAOTBO010000000
VERSION     DAOTBO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18918
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_18918_cov_5.594762"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..9397
                     /locus_tag="PLE88_07215"
     CDS             <1..9397
                     /locus_tag="PLE88_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76290.1"
                     /translation="RGGNGGNGSDAKGQGDGGNGGNGGIGGDLRAPDSCGGAVYYGKN
                     TKATIVGCRFENCRVIAELSGDYSGGNGGNGGNGEGDGSVGGNGGHGGPAYFIPDKMR
                     LIGNVNATGGTGGNGGPRGRGGNGGYRNGTGGAPGGISYNRSNPNTGIFPSNIYYMAY
                     YWEDTTNVFNYPDSPEDYTDFGFQWEWEPVLVNTETLDYYDPANPDVSLGTYDRFEYQ
                     TVLMINSPFSPYYGLVASTMFVEPTLTVETDPNTNEPVLVADYDNPVITPGQTELIKS
                     LTPTNSEQPNAGACAGANYYGQNSIITMRDTTVSFNSSFANHGGGELYDKGCRATFEN
                     CIFEGNTTLYETAAEVDYKFEGYGGGIFADQPVSMIFNGCQFSANSGFAGGALYCNFG
                     PSDANYLPELQITDTTFTANRADYHFIYSYGGAVYAGNSLTPYEEYYFNYLKDYRYTN
                     APDYALFFRSDLTATEDFTSFIASVYAHSHGEVEFTHPITTQLWDDRVEADYILKPWG
                     EPDKKYPVYPVSVEGSIFDRNLSPYGAGLYLDACDADVSGCHFEANTACVGAGGFVFA
                     SDITAGNNTFFYNTGREIESLGYQSQADNSTVEITGKAPALYLSNSDSFLISNRFVAN
                     ESGGLAGALFINGLSPTGYPQSVFNCLFVENSSGYAGGALIADGGTDVEVLNCTFAEN
                     LVSDRYGYGGGIVAHDAYMSITNTILWGNLAALGPQLCIGDPVEVLQEYDPFYVPATT
                     VFVDFSDLQGGQNDVYIDWNGWPWLEYGDNNIEDNPATLEDESDPQFIEVTDPNDATG
                     RTFYLAEIAAGQLTDSPCVDSGTPTHFIYDPAVGGFVTVPDTASWLQEKLGYEMTTRT
                     DHAADTGIINMGYHYDASLPVLQYSLTTSVFISDREPHGTIQQGSSEKHVEPLTLTYP
                     QGTVVLLQAFAEPEDLYRVARWIGSDDDRSFDSTNTITLTGNRTVQVEFELAVPKYLY
                     VPESYDTIEDAVTAARSGDTIVLTPRPSQPYYISNPDGIDFGRDKYGRPKEIVITSVD
                     PNDPLVVAQTIIDCRGSRYSSKRAFRFSSGQTAQTRIEGITIRNAFTARIGLSAAVPL
                     GRWPWWTGDASWYGGPIIQEWVEDPPNPSPLPPMRALSGMDATGDSYGGAILCENGSS
                     PVIRNCVFENCTVAGGIGGDGANGNYPANMQTDQDLDSQSGGHSGKGTGDGYGGAIAV
                     RSQSSPQIVNCTFRNNRATGGWGGIPGNAGLSYNNGRYGWGGNDPAGLEYAAQYFGYN
                     PQAGYGEGDGHGGAIYVEAGCKPQIKECIFENNYARPGYVSAGGREAAGNDYPQPFDS
                     QTVGAQGLPWGNAGARAGRNGILLTYDTIAGGAIYLEDEADILLENCIFKQNQAYEVY
                     TYNDAPVATRGGAVHSEPNVVLTVVDSMFRENLAGALYCSTGSTLTVDKAQFINNLTY
                     LDAASPTATFDIAGGITVAQDANAVSMITNCQFLGNTSHTGGAAVRADGDMYFENCIM
                     NGNQSLVDGGAVYSFVPLPSPDTHTTKLTFVSCELSGNTAQGYGGAVFAKNCILEVTD
                     SFIVHNTAYSGGAVRISEGELTMGGCLIYANEATGLNAGSYRTVTAEGYGGGVHITDT
                     PFSIVDTRFQNNKANGIISAGGGLCITGSQTYYKQNLLNCLFADNQSDNIGGGIACQL
                     YVDLQLDNCTFANNVSGGQTADWSPRIPYVAGQFVMASDGYKYKSKIGTVANPNLGHD
                     PVEDTAKVYWERAEGGGALHIDHLSRAVLKNSIISGNKGIAVYEKLGGNSKVSYTLFY
                     ANAGGDMHNGADGVVYSGVAAPEYANNKIGDPLFAAGPLGMYYLDQTASPAVNAGSIT
                     AAAAGLDVFTTSPSGSLDTGMVDLGYHYQDAAGLASYSLSTSFRDEAGTAVNNAGTIT
                     TVINGGTVIPASGMFKRGAILELTANVDSAYYLTGWSGGTINDNTTAPKNTVLMARNK
                     DIAVLVRLRRTLYVGTSAQYDTLGDAIYAAQDGDTILVAPGEYTSPSQFPSIANSIFL
                     TGKKITIAGFNPDDENVVRATVFRDYRFELADLDNQTVIDGITMNQCWMYMENSDPII
                     RNCVFSECQFANSTEVHSGTVPAGTDGYHQPPIYGGAIAMFNSSPKIINCTFENNSVR
                     GANGENGFAGGPTHPTGGDGGWPGAAYGGAVYCVFSSKPEFIRCTFTGNEVFGGNGGN
                     GANGWVNAGNTYDGGRGGGWLYDPEMEEYLRLNAFDTWDGWANNSYGDKYSAWSIYAN
                     YYGVYDIDVWARWFGWGDNYTSWEDFMADYNANPYDPLGDPYDQLLDVWRYSGFGGAV
                     YCEFDSDARFEECIFENNQSHGGLTGVGGVIADQTSRPDRQLNIPTAGGAVYAAHDSD
                     LTFDRCVFRNNLADKSMVQLPHTYQVSFGGAVAYEFNCTVTFDDCDFQDNDATVGGGM
                     YGRDSFATIADCNILSNQAYIGAGIYLQMEEADITNTVFRANRAWNSDVSGTIDLAGC
                     GAGLFANVLNLNIRDSVFVQNAADISGGGLMVTGVSDEPTDIFNCLFVHNTAGRDGAG
                     ASVNWHSNARFGSCTFADNSVLGLADFTASGGGLYIGYNSTAQVIDSIFWGNTATQGE
                     SISVATGFASEPRPSTLAVSYTDVMNYPSANAIYVASGCTLDTGAGIFSGLPMFEAPL
                     EADDIAQQYYLNQTASQCKDKGSQTSVQAGLDSYTTSITGARDKGLLDLGYHYLVSPR
                     VQCSIIDEALWLSGRIDLGDLLAFADRWLNAGLVCAESNNWCGGADLNYDGDVNLDDM
                     SNISVCWLVEDTEPPFPNPAQWAMEPNAIPDTYGTIEMLAAVHHDAWWPDSYLEYYFD
                     CIQPPDGPDRGWSTNPYFKVSGLKPGGYKYVLRVRDGSGNMTADSVEADVIPGQKTTL
                     PQAQWLNPPFVMNAQFAIQMEALAYENFGTIGIPVPSLPSGYVIKYQYEYRGTAPGGD
                     SRAYMADRIYIDTGMVAGQTYTYRVRMGLFYEPGDGTSTKIADGPWSEEASVVAIAPD
                     LEPPQPNPAQHASGSPFQIYVASQGVYYHVVTAVAATDPSDVEYKFVCSDSSASSGSS
                     KDPDGIEWRNADNVAGRFYPNGTAQVPNQYWSRIGLVNQTDKVWYVITRDRSPNRNTG
                     QTSVGRTIASPEP"
     gene            9619..10341
                     /locus_tag="PLE88_07220"
     CDS             9619..10341
                     /locus_tag="PLE88_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM76291.1"
                     /translation="MIQVQHLRRCFGPVVAVDDISFTAKQGDVLGFLGPNGAGKSTAM
                     KMLACFLAPDGGTASICGRDILTDPVGVRQVIGYLAENAPAYNEMTVAGFLNFVCDAR
                     RIKGRARREALDRILSMCSIESVYHQTIETLSKGYRRRVGLAQALIHDPKVLILDEPT
                     DGLDPNQKYEVRQLINQMAKEKCIIVSTHILEEVEQVCNRTIIIARGRILVDSTPQKL
                     VQEYGCSLDEVFRRITKTKKSA"
     gene            10375..11106
                     /locus_tag="PLE88_07225"
     CDS             10375..11106
                     /locus_tag="PLE88_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM76292.1"
                     /translation="MQSFLAVLKRELKGYFATPVAYVFLVIFLFFAGYLPFRDGFFEI
                     RQADLRLFFTNLPLLFVFMVPSTAMRLWAEERKTGSIELLLTLPITVPQAVLGKFMAA
                     WIFLGIALLLTLPLPAVVVYLGDPDMGQIITGYLGALLMAGAFLAIGSFFSALTKNQV
                     ISFILSVVACAILVFAGNPTTLDYLSSNVSSAVVGIVETMSFQTHLDSMMRGVVELKD
                     AAYFIILIAAWVYACTLILEERKAR"
     gene            11106..13091
                     /locus_tag="PLE88_07230"
     CDS             11106..13091
                     /locus_tag="PLE88_07230"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOM76293.1"
                     /translation="MNRMMRTVLGIFFAAVIAVSAIWIIQNLGRQLRLDITERKLYTL
                     SQGTKEILADLKQPISMKLFYTKTAAMKAPDQIRFYNNYFTYVKALLEEYQAQSNGKI
                     KLDVIDPRPYSDEELAAIRYGLRRFNITEDESFFFGLVVQTQFGVTKIIDFFSPDRQE
                     FVEYDISYLIDTAVTRQKKRLGVLSSLPVMGDSEYMIRMMQMQGQQGRRKWGIISHLE
                     KQFEVTSIPADAGEITDVDLLLVIHPKDLSEKTQFAIDQFVLKGGRAIICVDPHSIAD
                     APDMQQQFAGGHRSASGLPRLLKAWGLEMPEMKFAGDRVLAVTGSPNPAQRPEKILSI
                     MRLSASEKCFNRDHPISAQLNEVTVVFPGVLNVLEADAEEPKLEHIPLLMTSARGNVW
                     SVSSPYELMSPDYRKFMQDFRDGDKPVVIGYFVSGNFKSAFPDGIEVAEENTDESGKE
                     EQSGQEESDKPKMKKITGLTQAEQPCAVVVLADVDLLCDMVAYQRTFFGLAAMGDNSS
                     LILNTLENLCGSSRLISIRSRGNYKRPFVVVDQIEAKADAETAEEEAAITAQIKGFEQ
                     QLNEKLRSLEGENKGELINQTILKEKQQIELKLHEAEKRLREVKMRKREQIEKLKERM
                     RFYCTIPGPILTLVIAVVLGVTRAVKRRRYISHASDA"
     gene            13117..14250
                     /locus_tag="PLE88_07235"
     CDS             13117..14250
                     /locus_tag="PLE88_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOM76294.1"
                     /translation="MSDRKLAILGIAALIMLGGAILQSHMGRKVNTAVFSSSPLVEGL
                     PIEAVSAVTITSEKGTQTVRLQKKDNVFVVAAKDNYPADVSKINTLISHCLDIRTREK
                     ITSDPANHADLKVTPETARYVIAFQDKDGKDIVGLAVSESDENGNSFARLLNSDDVYT
                     VSDVPWIDTSVMGYINTKLLEVQQDKVSSVAVRTPEDTYVLAAAEEGGEIRLENMPQG
                     KQYKGTTYKTVFNALSYLRFDDVMAAEKAPADLDFNSVYTCKLKDLTVYKVQLAKQGD
                     KTYAKVSADYLDKTEVTKTVGQVESEEELKKKEAKLLAIDAVNAFNQKHQGWIYVIPS
                     YKSGDLSKPLAELIEDIPTKPQAAESDAAEKAADMPADPNAAA"
     gene            complement(14306..16582)
                     /locus_tag="PLE88_07240"
     CDS             complement(14306..16582)
                     /locus_tag="PLE88_07240"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOM76295.1"
                     /translation="MNQTYFEAIAGELSIQSKQVEAVAGLLAQEATVPFIARYRKELT
                     GGLDEIAITTIRDRLVQMMELSQRKQAILKSLAERNLLTPELSEQIEQARTMTAVEDI
                     YLPYRPKRRTRAMIAKEKGLEPLASLLLAQDQQTDPTAAALAFISTEKGVEDAEQALA
                     GARDILAEQISEDKTAREQMRRLYFEQGVFVSKVIKDKETEGRKFENYFDWQEPVASA
                     PSHRILAMRRGEKEGFLTLRIAVPQEAALGILRTLFVRNNSPCAVHVEAAIEDGYRRL
                     LSASMETEVRLETKKRADAEAIRIFAENLHQLLMAPPLGQKRIMGVDPGFRTGCKIVL
                     LDKQGKLLHHDVIYPHNSTEQAEKEARKICQLCEQFRIEAVAIGNGTAGRETETFFRS
                     AGLPSAILIVMVNESGASVYSASEAAREEFPDEDITVRGAVSIGRRLMDPLAELVKIE
                     PKSIGVGQYQHDVDQTLLKQGLDDVVISCVNKVGVEVNTASKQLLTYVSGLGPQLAQN
                     IVNYRNEFGPFSSREALKQVPRLGPKAFEQAAGFLRIRDSENPLDASAVHPESYWVVE
                     KMASDLKCTIRELMTVEAYRNRINLNSYITDTVGLPTLTDILNELAKPGRDPRDKFET
                     AVFAEGITKLEDLKPGMKLSGIVTNITAFGAFVDVGVHQDGLVHISELSNQFVKDPAD
                     IVKVHQKVQVTVLEVDLEKRRIKLSMKEQTPKQPPAKEQGPKTKPPSSSKERHSKGRR
                     VSKIDPNKPLIDQLTLGM"
     gene            complement(16690..18231)
                     /locus_tag="PLE88_07245"
     CDS             complement(16690..18231)
                     /locus_tag="PLE88_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76296.1"
                     /translation="MVPVTISYEDFKALISGRIKRYFTAVEYVTKHAGDKKFLTRPLL
                     GELLSQASQIEELLDSYGARNNCRWAKFRSLTAAIKLFSNVSYELLHIQHSIPAYRLL
                     PVKQDFAKYTEETLYICEEILIEAAKRLLEVAGQLHLPTENLSIPETSYSETLPPGRL
                     PHDLAMRKIEAVSQTVSMLATAFLNLAAESRNALPGRLTKPQAYLCELTGPVSEEKLR
                     SLELRFHNLQSLYDTYVSTTETEVFDNDLPVLRGHISVVLHLLRTARDLAHYYERHAS
                     PVAHPIVKVSKRLVEPEKLLDVLINYCINFVSLYLNCAELLCQDMLKRYTEIGHIEVP
                     VPQYRGFHVRPSTLVSKLVLHYGSDVKMKLGDDSYDASSPLELFRANEKINAHKRRSL
                     AQEIICLDLVPSVPGDKSLKTIVRDIIMTLAERGKIILYEQPLKIEDKPYSPDTKLLE
                     QVIDEIAKLQMMGKIDIGTDIRAVFIGDKRVLQDIQLLAENGYGEDIVGNNIPLPERL
                     AYLRQ"
     gene            complement(18346..>18918)
                     /gene="xth"
                     /locus_tag="PLE88_07250"
     CDS             complement(18346..>18918)
                     /gene="xth"
                     /locus_tag="PLE88_07250"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOM76297.1"
                     /translation="ILSKIAPQDVVVKLEQDTSYQARFLKAKIGKIIVVNTYAPQGMD
                     VNSDKFRYKLDWFNWLRLYFEKNHTPDQPIIWAGDLNVALQDIDVYDPKGLWGHVCYC
                     ETVQKALLEVMSWGFVDIFRLKHPEPGHYTFWDYRVLNAFKRNLGWRLDYIMTTSSLA
                     DKCTDCWIDTLPRGWEKSSDHTFLVADFDI"
BASE COUNT         4468 a         4637 c         5130 g         4683 t
ORIGIN      
        1 ccgcggcggc aacggcggca acggcagcga tgccaaaggg caaggcgacg gcggcaacgg
       61 cggcaacggc ggcatcggcg gcgatctgcg cgcgccggat tcctgcggcg gagctgttta
      121 ttacggaaaa aataccaaag ccaccatcgt tggatgccga ttcgaaaact gccgcgtcat
      181 tgctgaattg agcggcgatt attccggcgg caacggcggc aacggcggca acggcgaggg
      241 cgacggctca gttggcggca acggcggtca cggcggtccg gcatacttca ttccggataa
      301 gatgcgtctt atcggcaatg tgaatgcaac cggcggcacc ggcggcaacg gcggtccgcg
      361 cggccgcggc ggcaacggcg gctatcggaa cggcaccggc ggtgcaccgg gagggattag
      421 ctataatcgg tccaatccca acacgggaat cttcccttcc aatatctatt atatggccta
      481 ttattgggaa gataccacca atgtgttcaa ttatccggat agtcctgagg attataccga
      541 ttttggattc cagtgggaat gggaacctgt acttgtcaat acagaaactc ttgattatta
      601 cgatccagcc aatccggatg tgtctctggg aacctatgac cgatttgagt accagacggt
      661 tcttatgata aacagtccct tctcgccgta ttatggtttg gtcgccagca caatgtttgt
      721 tgaaccgacg ctaacggtcg agacagatcc caacaccaac gaacctgtat tggttgccga
      781 ttatgataat cctgttatta cgcccggcca gacggagctg attaaaagtc tgaccccgac
      841 gaattctgaa cagcccaatg cgggggcttg tgccggagcc aattactatg gccaaaacag
      901 cattatcaca atgcgggata cgactgtatc ctttaactcc agtttcgcca accacggcgg
      961 cggcgagctg tatgataagg gctgtcgtgc cacatttgaa aattgcattt ttgaaggaaa
     1021 tacaacgctt tatgagaccg ccgctgaggt cgattataaa tttgaaggat acggcggcgg
     1081 catttttgca gaccagccgg tcagcatgat tttcaacggc tgtcagttca gcgccaacag
     1141 cggctttgcc ggcggtgccc tttattgcaa tttcggtccg tcggatgcca actatctgcc
     1201 ggagcttcag attacagaca caaccttcac cgccaacaga gccgattatc actttatata
     1261 ttcctacggc ggagcggtct atgcaggcaa ttcgctgact ccttatgaag agtattattt
     1321 caattatctt aaagactatc gctatacaaa cgcccccgac tatgccctct ttttcaggtc
     1381 tgatctgaca gccacagagg atttcaccag tttcattgca tcggtatatg cccattcaca
     1441 cggggaagtg gaatttactc acccgataac tacccagctg tgggatgata gggtggaagc
     1501 cgattacatt ctcaaaccgt ggggtgagcc ggacaagaag tatcctgttt atcctgtttc
     1561 tgttgaaggc agtatatttg acagaaatct gtctccctat ggtgccggct tgtatctgga
     1621 tgcctgcgat gcggatgttt cgggatgtca ttttgaggcc aatacagctt gcgttggagc
     1681 cggcggtttt gtgtttgcca gcgatattac agccgggaac aacacgttct tttataatac
     1741 aggccgcgaa attgaaagtt tgggttacca gagtcaggcc gataacagta ccgttgagat
     1801 aaccggcaaa gccccggcgc tgtatctttc caacagcgat tcgtttttaa tcagcaaccg
     1861 gtttgtcgcc aatgaaagcg gcggcttagc gggggcgctg tttatcaatg gtttgtctcc
     1921 gacgggctat ccgcagtcgg tatttaactg cctgtttgtc gaaaactcat cgggttatgc
     1981 cggcggtgct ctgattgccg atggcggtac ggatgttgaa gtgctgaact gcacgtttgc
     2041 tgaaaatctg gtatccgacc gctacggcta cggcggcggt attgtcgctc atgatgccta
     2101 tatgagcatt accaatacca tcctctgggg caatctggcg gctttgggac cgcagctgtg
     2161 cattggtgat ccggtggaag tgcttcagga gtatgatccg ttttatgtcc cggcaacgac
     2221 ggtctttgtg gacttctctg atctccaagg cggccagaac gatgtgtata ttgattggaa
     2281 cggctggccg tggcttgaat atggagataa caacattgaa gacaatcccg ccacgctgga
     2341 ggatgaatca gatccgcagt ttatagaagt aaccgacccc aatgatgcta ccggccggac
     2401 gttttatctg gcagaaattg ccgccggtca gctgactgac agcccgtgtg tggattccgg
     2461 aacgccgacc cactttattt atgatccagc tgtcggtgga tttgtgactg tgccggatac
     2521 cgccagctgg ctgcaggaga agctgggcta cgagatgaca acccgaaccg accacgcggc
     2581 tgatacgggc atcatcaata tgggctatca ttatgatgct tccctgccgg ttctgcagta
     2641 ttcgttaacc acatcagtct tcatttcgga ccgtgagccg cacggtacca tccagcaggg
     2701 cagcagcgaa aagcatgtgg agccgctgac tctgacctat ccgcagggca cagtggttct
     2761 gctgcaggca tttgctgaac ctgaagatct gtatcgggtg gcgcgctgga tcggttcgga
     2821 tgatgaccgt tcctttgatt cgaccaatac cattaccctg accggcaatc gaaccgttca
     2881 ggtggagttt gaattggctg tgcccaaata cctgtatgtg ccggaaagct acgatacgat
     2941 tgaggatgct gtcactgcgg cacgttcggg cgatacgatt gttctgacgc cgcgtcccag
     3001 tcagccctat tacatcagca atccggacgg gattgatttc ggcagggata aatacggcag
     3061 acccaaagaa attgtgatta cttcggtgga tccgaatgat ccgctggtgg tggcgcagac
     3121 aattattgac tgccgcggaa gccggtattc gtccaagcgg gcgttccgct tctccagcgg
     3181 ccaaacggct caaacacgca tcgaaggcat taccattcgc aatgccttta cagcgaggat
     3241 cggcttgagt gcggcggtgc ctctgggacg atggccttgg tggaccggcg atgcctcatg
     3301 gtatggcggg ccgattatac aggaatgggt cgaagatccg ccgaatccaa gcccgctgcc
     3361 gccgatgcgt gctttgtcgg gaatggacgc gacaggcgac agttacggcg gagcgattct
     3421 gtgcgaaaac ggcagttcac cggttattcg aaactgcgtc tttgaaaact gcaccgttgc
     3481 cggcggtatc ggcggcgacg gagccaacgg gaattatccg gccaacatgc agacggatca
     3541 ggaccttgac agccagtctg gcggtcactc cggaaagggt accggcgatg gctacggcgg
     3601 ggcgatcgca gtgcgtagcc aaagcagccc gcagattgtc aattgcacct tccggaacaa
     3661 ccgcgcaacc ggcggctggg gcggtatccc gggtaatgcc ggactgtcct ataacaatgg
     3721 gcggtacggc tggggcggga atgatcctgc cggtctggaa tatgcggcgc agtatttcgg
     3781 ctataatcct caggccggtt acggtgaagg tgacggccac ggcggtgcta tctacgtcga
     3841 agccggctgc aagccgcaaa tcaaggaatg tatatttgag aacaattacg ctcgtcccgg
     3901 ttatgtcagt gccggcgggc gtgaggctgc aggcaacgat tatccgcagc cgtttgacag
     3961 ccagacggtc ggagcgcaag gtctgccgtg gggcaatgcc ggagcccggg ctggacgcaa
     4021 cggcattctg ctgacctatg atactattgc cggcggagca atctatcttg aagacgaagc
     4081 cgatatattg cttgaaaatt gcatattcaa gcagaatcag gcctatgaag tctataccta
     4141 taatgatgct ccagtggcaa cccgcggcgg tgctgttcac agtgaaccga atgttgtctt
     4201 gactgttgtc gatagcatgt tccgtgagaa tctggccggc gcgctgtatt gttcgaccgg
     4261 atcaacattg actgttgata aagctcagtt tatcaacaat cttacatatc tggatgccgc
     4321 atctccgacg gcaacctttg atattgccgg cggtattacc gttgcacagg atgccaatgc
     4381 cgtatcgatg attaccaatt gccagttcct tggcaatacc tcccatactg gcggtgcggc
     4441 tgtccgggct gatggtgata tgtactttga aaactgcata atgaacggca accagtcact
     4501 ggttgacggc ggtgcagttt acagctttgt gccgctgcct tcgccggata cccataccac
     4561 caagctgacg tttgtcagct gcgagctgag cggcaacacg gctcaaggat acggcggcgc
     4621 tgtgtttgca aagaactgca ttcttgaagt cactgacagc ttcatcgtcc ataataccgc
     4681 ctacagcggc ggtgctgtgc gcatttcaga aggcgaactg acgatgggcg gttgcctgat
     4741 ttacgccaac gaagctaccg gtctgaatgc aggctcctac cgcactgtta cggccgaagg
     4801 atacggcggc ggtgtgcata ttaccgatac gccgttctcc attgtggata cgcgcttcca
     4861 gaacaacaag gctaacggca ttatttctgc cggcggcggt ctgtgcatta ccggaagcca
     4921 gacctattat aagcagaatc tgctgaattg cctgtttgcc gacaaccagt cggataatat
     4981 cggcggcggc atagcatgcc agctgtatgt agatcttcag ctggacaact gcacgtttgc
     5041 caacaacgtc agcggcgggc agactgccga ctggagtcct cgaataccct atgtagcggg
     5101 ccagtttgtt atggcaagcg acgggtataa gtacaagtcg aagatcggta cagtagcaaa
     5161 tcccaatctc ggccatgatc ctgttgaaga cactgcaaaa gtgtactggg aaagagccga
     5221 aggcggtgga gcactgcata tcgatcacct gtcccgtgcc gtactgaaga actcgataat
     5281 cagcggcaac aaaggcatcg ctgtgtacga gaaattgggc ggcaattcga aggtttctta
     5341 taccttgttc tacgccaatg caggcggcga tatgcacaat ggcgccgatg gtgtagtgta
     5401 ttccggcgta gctgctccgg aatatgccaa taacaagatt ggggatccgc tgtttgccgc
     5461 aggtccgttg ggaatgtatt atctggacca gacagccagc ccggctgtga atgcaggcag
     5521 cattacggct gccgcagcag gtctggatgt gttcacgacc agcccgtccg gctcgctgga
     5581 taccggaatg gtagacttgg gataccacta tcaggatgct gccggattgg catcctacag
     5641 tctgtcaacg tcattccggg atgaagccgg tacggcggtc aataacgccg gcacgatcac
     5701 aacggtcatc aatggcggaa cggttattcc cgcttctgga atgttcaagc gcggtgcgat
     5761 tcttgagctg acagcaaatg ttgacagtgc ttattatctg accggatggt ccggcgggac
     5821 gattaatgat aataccacag cgccgaaaaa tactgttctg atggcacgca acaaggatat
     5881 tgcggttctt gttcgtctgc gccgcacact ttatgtcggt acatcggcac aatacgatac
     5941 ccttggcgat gcgatttatg ctgcacaaga cggggatacg attctggtgg ctcccggtga
     6001 atatacctcg ccgagccaat ttccgtctat cgccaacagc atcttcctga ccggcaagaa
     6061 gattacaatt gccgggttca atccggatga tgaaaatgtt gtgcgtgcta cggtattcag
     6121 agattaccgc tttgaactgg cagatttgga taatcagacg gtcattgacg ggattacaat
     6181 gaaccagtgc tggatgtata tggagaattc agatcccatt atccgcaact gcgtgttcag
     6241 tgaatgccag tttgccaatt ctacagaagt gcattctgga acagtgccgg caggtacaga
     6301 tggctatcac cagccgccga tttatggcgg tgctattgcg atgtttaaca gctcgccgaa
     6361 gattatcaac tgcacctttg agaataattc cgtccgaggc gcgaatggtg aaaacggctt
     6421 tgccggcggc ccgacgcatc ctaccggcgg tgacggcggc tggccgggtg ctgcctatgg
     6481 cggtgctgtc tattgtgtct tcagcagcaa gcccgagttt atccgctgta cctttaccgg
     6541 caatgaggtc ttcggcggca acggcggcaa tggagccaac ggctgggtga acgctggaaa
     6601 tacctatgac ggcggacgcg gcggcggctg gctgtatgat cccgaaatgg aagaatatct
     6661 ccgcctcaat gcctttgata catgggacgg ctgggccaat aacagctatg gcgacaaata
     6721 cagtgcttgg tccatctatg ccaactatta cggcgtttat gatattgacg tttgggcccg
     6781 ctggttcggc tggggtgaca attacaccag ctgggaagat tttatggcgg attacaatgc
     6841 caacccgtac gatcctctgg gtgatccgta tgaccagttg ctggatgttt ggcgctacag
     6901 cggattcggc ggtgcggttt actgcgaatt tgacagcgat gcacggtttg aagaatgcat
     6961 ctttgaaaac aaccagtcgc atggcggcct gacgggagtg ggcggagtca tcgccgacca
     7021 aacctcacgg ccagaccgtc agcttaatat accgacggca ggcggggcgg tttacgccgc
     7081 tcatgacagc gatttgacgt ttgaccgctg tgtcttccgg aacaatctgg ccgataaatc
     7141 gatggtccag ctgccgcata cataccaggt cagcttcggt ggtgcggtgg cctatgaatt
     7201 taactgcacc gtaacctttg atgactgcga tttccaggac aatgatgcga cagtcggcgg
     7261 cggtatgtac ggccgtgact cgtttgcaac catagcagac tgcaatatct taagcaatca
     7321 ggcctatatc ggagctggta tctacctcca gatggaagaa gcagacatta ccaacacggt
     7381 attccgtgcc aaccgcgcct ggaattccga tgtctccggt acgattgatt tggccggctg
     7441 cggggcagga ttgtttgcca atgttctcaa cctgaatatc cgcgacagcg tctttgtgca
     7501 aaacgcagcg gatatttccg gcggcggttt gatggtgaca ggcgtgtctg atgaacccac
     7561 ggatatattc aactgcctgt ttgttcataa cacagccggc cgtgacggcg cgggtgcctc
     7621 tgtgaactgg cattccaatg cacggttcgg cagctgcacg tttgccgaca acagtgtcct
     7681 cgggctggct gactttacgg ccagcggcgg cggcctgtac atcggctaca acagcactgc
     7741 ccaagtcatt gacagcatct tctggggcaa tacggctaca cagggcgaaa gcatctcggt
     7801 agccaccggt tttgcctccg aaccgcgtcc gtcaacactg gcggtttcgt acaccgatgt
     7861 tatgaattat cccagcgcca atgcaattta tgtggcttcc ggctgcaccc ttgatacagg
     7921 tgccggcatc ttcagcgggc ttccgatgtt tgaagcgcct cttgaggccg acgacatagc
     7981 tcagcagtat tatctgaacc agacggcaag ccaatgcaag gacaagggaa gccagacatc
     8041 ggtgcaggcg ggattggata gttacacgac cagcatcacc ggggcacgcg acaaaggact
     8101 gctggatttg ggttatcact accttgtgtc gccgagagtc cagtgcagca tcattgatga
     8161 agcgctgtgg ctgagcggac ggattgattt aggtgatttg ctagcctttg cggatcggtg
     8221 gctcaacgcc ggtcttgtat gtgccgagag caacaactgg tgcggcggag ccgacctgaa
     8281 ttatgatggg gatgtcaatc tggatgatat gtccaacatc tcggtctgct ggcttgtgga
     8341 agatacagag cccccgttcc cgaatccggc acagtgggcg atggaaccca atgcgatccc
     8401 cgatacatac ggtacgattg aaatgctggc ggctgttcat catgatgctt ggtggccgga
     8461 ttcgtatctg gagtactatt tcgattgcat ccagccgcct gacggaccgg ataggggctg
     8521 gtcaaccaat ccgtacttca aggtttccgg attgaagccc ggcggctata agtatgtcct
     8581 gcgggtgcgt gatggaagcg ggaatatgac agccgattcc gttgaggccg atgtaatccc
     8641 cggccagaag actacactgc cgcaggccca gtggctcaat ccgccgtttg tgatgaatgc
     8701 ccagtttgcc attcagatgg aagcgctggc ttacgagaac ttcggaacaa tcggcattcc
     8761 ggttccatcg ctgcccagcg gctatgttat caagtaccag tatgaatacc gcggtacggc
     8821 ccccggcggc gacagccgcg cctatatggc agatcggatt tatatcgata ccggcatggt
     8881 tgcaggccaa acctacacgt atcgtgtacg gatgggattg ttctatgagc ccggcgatgg
     8941 gacttccact aagattgccg atggtccgtg gtcagaagag gcttctgttg tggccatcgc
     9001 ccctgatctt gagccgccgc agcccaatcc ggctcagcat gccagcggtt cgccgttcca
     9061 gatttatgtg gcttcgcagg gtgtgtatta tcatgtggtc acggcggttg cggcgacaga
     9121 tcccagcgac gtggaataca agtttgtctg cagcgattcg agtgccagca gcggctcgtc
     9181 caaggatcca gacggcatcg agtggcgaaa tgcagacaat gtagccggcc ggttctatcc
     9241 gaacggaact gcacaagtgc ccaaccaata ctggtcacgc attggattgg ttaaccagac
     9301 cgacaaagtg tggtatgtaa taacacgcga ccggtcgcct aaccggaata caggccagac
     9361 gtcggtcggc agaaccatcg cttcgccgga accataagcc ggatttaact gctaatcctg
     9421 tttttaggcg ggccgtcagt tatgcggccc gctttttttt atctgctccg gcagttgcaa
     9481 atcttgccca caaaagggct tgacagggca gggaaaaacc ggtaaaaaaa ccgaacatat
     9541 ttgcttgaat tgacgtagca aaaaattatt tttgggtaaa ttttggcagg ttcaggccgt
     9601 atcaagcagg agaagactat gatacaagta cagcatttgc gacggtgttt cggaccggtg
     9661 gtggcggtag atgatatttc ttttaccgcc aagcaggggg atgtgctggg ctttttaggc
     9721 ccaaacgggg ccggcaagag tacagccatg aaaatgctgg cctgctttct ggcacctgac
     9781 gggggaacgg ccagcatttg cgggcgggat atcctgacag atcctgtggg tgtgcggcag
     9841 gtgatagggt atttggcgga aaatgccccg gcctataacg aaatgaccgt tgcgggtttt
     9901 ctgaattttg tatgcgatgc ccggcggatc aaaggacgtg cccgtcggga agcgctggat
     9961 cggattctat cgatgtgttc gatagagtca gtttaccatc aaaccattga aaccctttcc
    10021 aagggctacc gccggcgagt cggtctggca caggcactta ttcacgaccc caaagtcctg
    10081 attctggatg agccgaccga tggactggac cccaatcaga aatacgaagt ccgccaattg
    10141 attaatcaga tggctaagga aaagtgcatt attgtctcga cccatatcct tgaagaagtt
    10201 gaacaggtct gtaaccgcac gattattatc gcccgcggga ggattttagt ggattcgacg
    10261 ccgcagaaac tggttcagga atacggctgt tcgctggatg aggtattccg cagaatcaca
    10321 aagaccaaaa agtctgcctg ataattgtgt ataaccgctg ttgggagatt caatatgcag
    10381 agttttttag ctgttctaaa acgggaattg aaaggttatt ttgccacacc ggtggcttat
    10441 gtgtttctgg tgatttttct ttttttcgcc gggtatctgc cgttccgaga cggctttttt
    10501 gaaatccggc aggcggattt gcggctgttt tttacaaatc tgccgctgct gtttgttttt
    10561 atggtgccct caacagcaat gcgtctgtgg gccgaagagc ggaaaaccgg ctccattgag
    10621 ctgctgctga ccctgccaat taccgtgccg caggccgttt tggggaaatt tatggcggct
    10681 tggatatttc tggggatagc gctgctgctg actctgccgc ttccggcagt agtggtctat
    10741 ttgggcgatc ctgatatggg gcagattatt accggctatc tgggggcatt gctgatggcc
    10801 ggggcttttt tggctatcgg ctccttcttc tccgcgttaa ccaaaaatca ggtgataagt
    10861 tttattctgt cggtagttgc ctgtgcgatt cttgtgtttg caggcaatcc aacaaccttg
    10921 gattatcttt cgtctaatgt gtcttcggct gtcgtgggga ttgtggaaac gatgagcttt
    10981 cagacgcatc tggattctat gatgcgggga gtcgtggaac tcaaggatgc cgcttacttt
    11041 attatcctga ttgcggcttg ggtatatgcc tgtacattga ttttggaaga aaggaaggca
    11101 cgctgatgaa caggatgatg cgtacagttc tgggaatctt ctttgctgca gtcattgccg
    11161 tttctgcgat atggattatc caaaatctcg gcagacagct tcgtttggat attacggaac
    11221 gcaagcttta taccctttcg cagggaacca aagagattct ggcagatttg aaacagccga
    11281 tatccatgaa gcttttctat acgaaaactg cggccatgaa ggcgccggac cagatacgct
    11341 tttacaacaa ttattttacc tatgtaaaag cattattgga agagtatcag gcccagtcga
    11401 atggcaagat taagctggat gtaattgatc cgcgtcccta cagcgatgag gagctggcgg
    11461 ccattcgata cggcttgcgc cggtttaata tcacggaaga tgaaagtttt ttcttcggac
    11521 tggttgtaca gacccagttc ggcgtaacca aaattatcga cttcttttcc ccggatcggc
    11581 aggaatttgt ggaatatgac atcagttatc tgattgatac ggcagttacc cgccagaaaa
    11641 aacggctggg tgttctcagt tctctgccgg tgatggggga ttctgagtat atgattcgga
    11701 tgatgcagat gcagggccag cagggccggc gaaagtgggg catcatcagt catctggaaa
    11761 agcagtttga ggttacatca attccggccg atgccgggga aatcaccgat gttgatttgc
    11821 tcttggtgat acatccgaag gatctgtctg aaaagacaca atttgccatt gaccagtttg
    11881 ttctcaaggg gggacgggcg attatctgtg ttgacccgca ttccattgca gatgctccgg
    11941 atatgcagca gcaatttgca ggcggacacc ggtctgcttc tggtctgccg cggctgctga
    12001 aggcctgggg attggaaatg ccggaaatga aatttgccgg cgaccgtgta ctggctgtta
    12061 cgggttctcc caatccggct cagcggcctg aaaaaatact cagcattatg cgattgagcg
    12121 cctcggaaaa atgctttaac cgggatcatc ccatcagtgc ccagctcaat gaggtaacgg
    12181 tcgtgttccc cggtgtcctt aatgttctgg aagccgatgc agaagagccg aagctggagc
    12241 atattccgct gctgatgacc tcggcacgcg gcaatgtgtg gtcggtgagc agtccctatg
    12301 aattgatgag tcccgattac cgcaaattta tgcaggattt cagggatggt gacaaaccgg
    12361 tggttatcgg gtattttgta tccggcaatt tcaagagcgc atttcctgac ggaattgaag
    12421 ttgccgagga aaacacggac gaatccggca aagaagaaca gtccggccaa gaagaatccg
    12481 ataagcccaa aatgaagaaa atcaccggct tgacccaagc cgaacagccc tgtgctgttg
    12541 ttgtgttagc ggatgtagat ctgctgtgcg atatggtggc ctatcagagg acatttttcg
    12601 gtctggccgc gatgggtgac aacagttcgc tgattttgaa tacccttgaa aatctttgcg
    12661 gctccagccg tctgatttca attcgttccc gcggcaacta caaacgtccc tttgtagtgg
    12721 ttgaccagat cgaggccaag gccgatgctg aaacggctga agaagaagcg gctattaccg
    12781 cccagattaa gggttttgaa cagcagttga atgagaaact gcgttctctc gaaggcgaaa
    12841 acaagggcga gttaattaac cagacgattt taaaggaaaa gcagcagatt gagctgaagc
    12901 ttcacgaggc tgaaaagcgc cttcgtgaag tcaaaatgcg caagcgcgag caaattgaga
    12961 aactcaagga gcggatgcgc ttctactgca caattccagg accgattctg acccttgtta
    13021 ttgcggttgt gctgggagta acccgcgctg tgaagcgccg ccgctatatc agtcatgcca
    13081 gtgatgcctg aacttaggaa ataacaggag ataccgatga gcgacagaaa acttgccatt
    13141 ttgggaattg ccgcattgat aatgcttggc ggggcgattc tgcagagcca tatggggcgg
    13201 aaggtcaata cagcggtctt cagcagttcg ccgcttgtgg aagggctgcc cattgaggcg
    13261 gtctcggctg ttacaattac cagtgaaaag ggcactcaaa ccgtacgtct gcagaagaag
    13321 gataatgtct ttgttgtggc agccaaggac aattatccgg ccgatgtcag caaaataaat
    13381 acactgatca gccattgtct ggatatccgc acgcgtgaaa aaattacctc ggatcctgcc
    13441 aatcatgcag atttgaaagt tactcctgag acggcacggt atgtaattgc ctttcaggat
    13501 aaggacggca aagacattgt gggcttggcc gtttcggaaa gtgatgaaaa cggcaattct
    13561 tttgcccgtc tgctaaactc cgatgatgtc tacacggtct ctgatgttcc ttggattgac
    13621 acctctgtga tgggatatat caatacaaag ctgctggaag tacagcagga caaagtcagc
    13681 agcgtggcag tgcgtacgcc cgaggatacg tatgttctgg cagctgcaga agagggcggc
    13741 gaaattcgtc tggaaaatat gccgcaaggc aaacagtata aagggacgac ttataaaaca
    13801 gtgttcaatg ctctcagtta cctgcgattt gatgatgtaa tggcggcaga aaaagctccg
    13861 gctgatcttg acttcaatag cgtttatacc tgcaagctga aagatctgac tgtctataaa
    13921 gtgcagctgg ccaaacaagg cgataaaacc tacgcgaagg tgtcagccga ttatctggat
    13981 aagacggaag taaccaagac tgtgggtcag gtcgaatccg aagaagaact gaaaaagaaa
    14041 gaggccaaac tgctggcaat tgatgctgtc aatgcgttta accaaaagca tcaggggtgg
    14101 atttatgtga ttccttctta caagtccggc gatttgagca agccgctggc ggaattgata
    14161 gaggatattc cgaccaaacc gcaggcggcc gaatcagatg cagccgaaaa agctgctgac
    14221 atgcctgccg atccgaatgc ggctgcataa atcccgccat ccaacagtgc tgtccgggca
    14281 ggggcgtgtt gccgctcctg cctttttaca ttcccagcgt taattgatca atcaagggct
    14341 tattggggtc gattttgctc acgcgtcgcc cttttgaatg ccgctctttg gaagatgacg
    14401 ggggctttgt ttttggccct tgttctttgg ccggcggctg cttaggcgtc tgctccttca
    14461 tggacaattt gatgcggcgt ttttcaagat ccacctccag aaccgtaacc tgtacttttt
    14521 gatgaacctt gacaatgtca gcagggtctt taacaaattg gttcgataat tcactgatat
    14581 gcaccagacc gtcctgatgt acccccacat ccacaaaagc tccgaaagcc gtgatattgg
    14641 tgacaatgcc ggatagtttc atgcccggct tgaggtcttc cagcttggtt atgccttcgg
    14701 caaaaaccgc tgtctcaaac ttgtcccgcg gatctcggcc gggtttggcc agttcgttga
    14761 gaatatcggt cagcgtcggc aggcctaccg tatcggtgat atagctgttc aaattaatgc
    14821 ggttgcggta agcctcaaca gtcatcagtt cacgaatcgt gcatttcagg tctgaggcca
    14881 ttttttccac aacccagtag ctttcaggat gcacagcgct ggcatcaagc ggattttcgg
    14941 aatcgcggat gcggagaaag ccggcggcct gttcaaatgc cttgggcccc agccggggaa
    15001 cttgtttgag cgcctcccgc gacgaaaagg gaccaaattc atttctgtaa tttacaatat
    15061 tttgggcaag ctgcggtccc aaacccgata cgtacgtcag cagctgcttg ctggctgtat
    15121 tgacttcgac gccgacttta ttgacgcagc tgatgacgac atcatccaat ccctgtttca
    15181 gcagggtttg gtcgacatca tgctgatatt gtccgactcc gatggatttg ggttcgattt
    15241 taaccaattc agccagcgga tccatcagcc gccggccgat ggagacagct ccccgcacgg
    15301 taatatcttc atccggaaat tcttctcgag ctgcttcaga tgccgaataa accgacgccc
    15361 cgctttcatt aaccattaca atcagaatcg cagaaggcaa tcccgcagag cggaagaacg
    15421 tttctgtttc ccgtccggct gtcccgttgc cgatggcaac tgcctctatg cggaattgtt
    15481 cacacagctg gcagattttc cgggcttcct tctcggcctg ttcagtgctg ttatgcggat
    15541 agattacatc gtggtgcagc agcttgccct gtttgtcaag cagaacaatt ttgcagccgg
    15601 tgcggaagcc ggggtctaca cccataatcc gcttctgccc cagaggcggc gccatcagaa
    15661 gctgatgaag attttctgca aagatgcgaa tggcttctgc atctgcccgt tttttggttt
    15721 ccagccgaac ctctgtttcc attgaagcag aaagcagccg ccggtagccg tcttctattg
    15781 ccgcctcgac atgaacggca caaggactgt tgttgcggac aaaaagcgtc cgcagaatgc
    15841 ccagcgctgc ctcttgcggg acggcaatcc gcaatgtcag aaatccttct ttttcgcccc
    15901 ggcgcatcgc cagtatccga tgggacggtg ccgatgctac cggttcctgc cagtcaaaat
    15961 agttttcaaa tttacggcct tctgtttcct tgtctttgat gactttggac acaaagacgc
    16021 cttgttcaaa atacagccgg cgcatctgct cccgcgcggt tttatcttca ctgatttgtt
    16081 cggccagaat atcacgtgct ccggctaaag cctgctcggc atcttcgacg cctttttctg
    16141 tgctgatgaa tgccagtgct gcagcggttg gatcagtctg ctgatcttga gccagcagca
    16201 gcgaagccag cggctccagc cccttttctt tggctatcat ggcacgcgtt cgccgcttcg
    16261 gacggtaggg caaatagatg tcttccactg ccgtcatagt ccgggcctgt tcaatctgct
    16321 ctgacagctc cggcgttaga agattgcgtt ccgccagcga cttgagaata gcctgttttc
    16381 gctggcttag ttccatcatc tgaaccaacc gatcccggat ggtggtaatg gcaatttcgt
    16441 caagaccgcc ggtcaattcc ttgcggtagc gtgcgataaa cggaacggtg gcctcttggg
    16501 ccagcagtcc tgccaccgcc tctacctgtt tggattgaat cgatagttcg cctgcaatgg
    16561 cctcaaaata tgtctggttc acacccattc ctttcgctta tgaatgaatc aattaaggat
    16621 gacggatatt ccggcaaaca tgaagaaaat gatcaggata ggtacttttg ttgtcatgcc
    16681 ggatgtgtgc tattgtctca gataagccaa ccgttccggc agaggaatat tattgccgac
    16741 aatatcttca ccatacccat tttccgccag aagctgaatg tcctgaagga cgcgcttgtc
    16801 gccgatgaat acagctcgga tatctgtgcc gatatcaatt tttcccatca tctgcaactt
    16861 tgcgatttca tcaataacct gctctagcag tttggtatcg ggtgaatagg gcttgtcttc
    16921 aattttcagg ggctgttcat agagaatgat ttttccgcgt tctgccagcg tcataataat
    16981 atcccgcacg atcgttttga ggcttttatc gcccgggaca gaaggaacca aatcgaggca
    17041 gataatttcc tgtgccagtg aacgccgttt atgggcattg attttttcat tggcgcggaa
    17101 cagctccagc ggagagctgg cgtcgtagga gtcatctccc agcttcattt ttacgtcgct
    17161 gccgtaatga agcaccaatt tggagaccag tgtggagggc cgaacgtgaa agccgcgata
    17221 ttgggggacg ggtacttcaa tatgtccgat ttctgtatag cgtttaagca tgtcctgaca
    17281 aagcagctca gcacagttca gatataacga aacaaaattg atgcagtaat tgataagaac
    17341 atccagcagc ttttcgggtt caacaaggcg cttgctgact ttgacaatgg gatgggcaac
    17401 cggactggca tgccgttcat aataatgtgc caaatcgcgg gcggttctca gcagatgcag
    17461 gactacgctg atatgcccac gcagaacagg caggtcatta tcaaaaacct ctgtttctgt
    17521 agttgaaaca taggtgtcgt agagggattg cagattatga aaccgcagtt caagcgaacg
    17581 cagtttttct tcgctgacag gcccggttag ctcgcataaa tatgcctgcg gtttggtaag
    17641 ccgccccggc agggcatttc ggctttcggc cgccagattc agaaatgccg ttgccagcat
    17701 ggagacggtc tgagaaacgg cctcgatttt tcgcatggct aaatcatgcg gaaggcggcc
    17761 cggcggcagt gtttcgctgt aggacgtttc ggggatagac agattttcgg tcggcagatg
    17821 cagttgcccg gcgacttcca gaagacgttt ggccgcttcg attaagattt cttcgcagat
    17881 atacagggtc tcttctgtgt attttgcgaa atcctgcttg accggcagca ggcggtaggc
    17941 cggaatcgaa tgctggatat gcagcagctc atagctgacg ttggaaaaca gcttgatggc
    18001 agctgtcagc gagcgaaact ttgcccagcg gcagttattt cgtgctccat aggaatccag
    18061 cagttcctct atttgtgagg cctgagacag caattccccc agcagggggc gggttaaaaa
    18121 ctttttatct ccggcatgct tggttacata ctcaactgcc gtaaaatacc gtttaattcg
    18181 accagatatt aaagctttga agtcttcata cgatattgtt acaggaacca taagcattgg
    18241 tctttgtatt caattagtaa tagtaggata agtttaataa atgccgcaaa caatacattt
    18301 tatcattaaa atctgtaaaa tacaatatgc attattcaag atatttcaga tgtcaaagtc
    18361 ggcaactaaa aaagtatgat cactggattt ttcccagcct cgaggtaatg tatctatcca
    18421 gcagtcggta catttatcgg ctaaagaaga agttgtcata atataatcta atcgccagcc
    18481 aaggttcctc ttaaaagcat tcaggactcg ataatcccag aaagtataat gtcccggctc
    18541 tggatgcttt aaccggaata tatccacaaa gccccaactc attacttcta agagtgcttt
    18601 ttgaacagtt tcacaatagc aaacgtgccc ccacaagccc tttggatcat atacgtcaat
    18661 atcttggagg gcgacattca aatcacctgc ccaaataatc ggctggtcgg gagtatggtt
    18721 tttttcaaaa tataatctca gccagttaaa ccagtctaat ttatatctga atttgtcaga
    18781 attaacatcc attccctgcg gcgcataagt atttacgaca attattttac ctattttggc
    18841 tttgaggaag cgtgcttgat atgaagtatc ttgttccagc tttaccacaa cgtcttgcgg
    18901 ggcaatttta ctgagaat
//
LOCUS       DAOTBO010000051        18544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2477_length_18544_cov_5.734707, whole genome shotgun sequence.
ACCESSION   DAOTBO010000051 DAOTBO010000000
VERSION     DAOTBO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18544
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_18544_cov_5.734707"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="PLE88_07255"
     CDS             complement(<1..1817)
                     /locus_tag="PLE88_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76298.1"
                     /translation="MSSDITPLVQQKVLTEECPAEQLSDSRCPEGRPRVLELLQRRSP
                     KDMLLRQLQQSQSTLAAVKESFEESQRTIKRLTRQKENLMGTCQDQFEYIGQLQHQLA
                     QLQQQKNQTDEQFQLLEAHSAEQACAIASLSEKLRWADIQIVSLEELVQKEREKNAAL
                     TDLQLQFQQVQQELRLALRQKENLAGTCRDQAEYIGELKDKISQLQLLQSDFCSIQEA
                     LQQEQMISRTAQSKLAEMEAARQQQIHQVNDLQSHIELLEQEKQKLACEVQGLQKQLE
                     KTDAELALKDEHIHLAEAQRQQLKTENAALLAELQQQKQENTALLNELAGLKDAGEKY
                     QAAASQCLLFEEQILKLSEQVKEKDIQLEAAAAELGDYRNRRQQWEEEAQRYTSLAEQ
                     LAQQCRMLEAKLQAAEDQLRQTSQAAHRQQEEFHHRIQAAECQRQEAHKQIDALKANL
                     DEACGQISTLQSRLAAAAEAEIKYQQTLEQVESLQKDIRNLHRRLESQTAETESARLL
                     LQESEKAKTILQEKLNQEKTAAKRLSLQKSGLERQLEDAFGALKQLRGSYLQLQQTYD
                     IQADELEKTCNQYQQQIEQMQGQLDGAVRQIQIQDSQIQ"
     gene            complement(2006..2320)
                     /locus_tag="PLE88_07260"
     CDS             complement(2006..2320)
                     /locus_tag="PLE88_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76299.1"
                     /translation="MSDYKKRCLVSSFWEYPQNSNPYQDGHAAAQGRIGRTYAEKNLE
                     AALIIKRLTAMFCFCCIQYFLKNRQSVLTFPDLTKASSGYIAAACSLQRAYKYAWELH
                     LL"
     gene            2745..2820
                     /locus_tag="PLE88_07265"
     tRNA            2745..2820
                     /locus_tag="PLE88_07265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2781..2783,aa:Pro,seq:tgg)
     gene            2854..3516
                     /locus_tag="PLE88_07270"
     CDS             2854..3516
                     /locus_tag="PLE88_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOM76300.1"
                     /translation="MIVNELFYSLQGEGLLAGVPSVFVRLAGCPLRCRWCDTKYAWNA
                     DAGQSMTAEQILEHIACYPTRYVVLTGGEPLMHEGIFDLIRAIHRKAYHLTVETAGII
                     SPAELPIDLMSISPKLSNTIADGTQSPPANPETVMRLIASYPYQLKFVVDDPKDLDEI
                     ACFVNKLAAVDPYKIYLMPQAANTADYLEKSRWLAEYCLQTGFLFSPRLHIMLWNGRR
                     GR"
     gene            3634..4092
                     /locus_tag="PLE88_07275"
     CDS             3634..4092
                     /locus_tag="PLE88_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOM76301.1"
                     /translation="MDKEEEKIRIDKKIAEFKDKCRLNGLRITPQRLAIYKALVETKT
                     HPTAEEVYSQVQKEFSNISLDTVNRTLLTLSEIGAAFIVEGTGQPRRYDGGLENHQHF
                     RCIKCGTIMDFYYEPYDKLDIPDYLQVTVLRKTVYLEGLCGICSGMPAFH"
     gene            4129..4701
                     /locus_tag="PLE88_07280"
     CDS             4129..4701
                     /locus_tag="PLE88_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOM76302.1"
                     /translation="MSLKGTQTEKNLLTAFCGESQARNRYTYFASQAKKEGYVQISAI
                     FEETANQEKEHAKRLFKFLEGGEVEICAAFPAGVIGTTEENLLEAANGENYETTQMYP
                     DFAQTAEKEGFKEIAAVFRKIAVAEARHRDRYQALRNNILNGQVFRRDTKVRWVCRNC
                     GYVHEGLEAPSVCPACNHPQDYFELEAVNY"
     gene            4873..5394
                     /gene="tpx"
                     /locus_tag="PLE88_07285"
     CDS             4873..5394
                     /gene="tpx"
                     /locus_tag="PLE88_07285"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOM76303.1"
                     /translation="MERKNTVTMKGAPLTLTGELVSVGQPAPDFTAAANDLSPVKLSD
                     YRGKVVVIVSVPSLDTPVCDIETRRFNQEAAEFGDQVAVLVVSMDLPFAQKRWCGAAG
                     VGNVRTVSDYQFAEFGTKYGLLIEQLRLLARAVVVVDKLGTVQYVQLVPEIASEPDYD
                     DVRAAIKKTSLSK"
     gene            5432..6613
                     /locus_tag="PLE88_07290"
     CDS             5432..6613
                     /locus_tag="PLE88_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOM76304.1"
                     /translation="MKMLADNVLAVGVQHWDRRLFDELIPLPDGTSYNAYLVKGSQKT
                     ALLDTVDPEKIEALIDHLMRAGAEKIDYIVSHHAEQDHSGGIPDMLMMYPEAKVVANP
                     KCKAMLMDHLHIDADVFIEVQDGQSLSLGDKTLHFLYTPWVHWPETMTTWLPEDKILF
                     SCDFFGSHQAASNLFVQDEARTLEAAKRYYGEIMMPFRSSIRSNLKKLEPLDIRLIAP
                     SHGPVYQKPELIIHAYKDWTDEDKVANAVVLPYVSMHGSTGKMATYLVEALMERGIDV
                     HPFNLSAADIGEIVISLVSAATVVMGIPTVLTGAHPSALYCAALVNALRPKTKFVGII
                     GSYGWGSKMVEQISSLLGNVKAEMLEPVLAKGQAQEETYSKLDALADTIAAKHRQLGI
                     A"
     gene            6661..7035
                     /locus_tag="PLE88_07295"
     CDS             6661..7035
                     /locus_tag="PLE88_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOM76305.1"
                     /translation="MAAKLEIYKCMACGNIVEVVHGGDGQLVCCGQPMKKMVENTVDA
                     AKEKHVPVIEKIDGGFKVTVGAVAHPMEEKHYIEWIELQADGQVYRQFLSPGQKPEAV
                     FWVKAEKAAAREYCNLHGLWKA"
     gene            7080..7598
                     /locus_tag="PLE88_07300"
     CDS             7080..7598
                     /locus_tag="PLE88_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOM76306.1"
                     /translation="MLNKKMEEALNKQINAETYSAYLYWSMSAALEKMNLPGFASWMR
                     VQAQEEMVHASMFYKHIIERDGTVRLAAVDAPPHQWSGVQQVFEAALKHEQHVSGLIN
                     SLFDLAVQEKDHAANLFLQWFVNEQIEEEKNARDIIGRLEIAGGTAGGLYMLDKEMAA
                     RVFTMPAAEQQP"
     gene            7627..8130
                     /locus_tag="PLE88_07305"
     CDS             7627..8130
                     /locus_tag="PLE88_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOM76307.1"
                     /translation="MAITFNADEIFEMAEEIERNGAAFYREAAAKASREDIKKMLLDL
                     AAMEEGHEQTFAQMRKKLSPAEKEQTTYDPDNEVAQYLRTMADFHGTEGKISPSEKLT
                     GNESMTEILKIALQAEKNSIAFYVGIKDLVPSRAGKGKIQTIIIEEMAHVSAIGGKLQ
                     SLRQAAD"
     gene            8211..9284
                     /locus_tag="PLE88_07310"
     CDS             8211..9284
                     /locus_tag="PLE88_07310"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOM76308.1"
                     /translation="MSKAAGGIGLMRSQPAMRKLLLGTCPCIGGAVYYFGWRSLAMIL
                     FCCAVGFFCEWLFCRRRKEPVTEAVFVTAVLFALAMPPGAGWHVLAAGMVFAIVVAKE
                     VFGGFGRNIFNPALAGRCFVYVCFPVAMTGIWYPPAQGSLGALLQWTSARQADGTIAV
                     TGATPMAHLKAGRLVRTPDAPYEAGIPFRIGSQETAAVRSLVLYKAQFFGRISGSMGV
                     TSALLILIGAVFLFWTRTASRTTILSVVLTYAVLNELLFRLGIAPVPGAVPAVLGGGF
                     LLGACFMATDPVSSPRTEWGRFYYGVLIAAATVVIRNFSIFNGGLMFAVLLGNMFAPI
                     IDYGVQTFGQRKKAKRILEENAG"
     gene            9281..9892
                     /locus_tag="PLE88_07315"
     CDS             9281..9892
                     /locus_tag="PLE88_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOM76309.1"
                     /translation="MKDKPWFAVVYMFVMTAFASSILIGFSRLTRSRVEANAQLQFER
                     AVVQVFPDIRFQHDEQIHRIFTQQFEKDPRSGAFRFLKDGRLAGYAVPFSGQGFWDKI
                     EGVIGVAADRRTITGIAFYRQNETPGLGARIEEEEFRSQFAGKRLAESPILLEIVPSA
                     QPLGPNSVHAVTGATQTSDRLQILLNANLHTWLEAVSNEGEAQ"
     gene            9889..10509
                     /gene="rsxE"
                     /locus_tag="PLE88_07320"
     CDS             9889..10509
                     /gene="rsxE"
                     /locus_tag="PLE88_07320"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOM76310.1"
                     /translation="MNSVKSPREIVLDGLWRDNPIFRQILGICSTLAVTNAVLNTAVM
                     CLSLTGVTALSCFTVSVLRNWTPRNIRMMVQTLIIAFYVIIVDLMLRAYWPQMSRNLG
                     PFVGLIITNCIVMGRCEAFAGSNPPFSSFIDGLFNGLGYSIVLLVISVFRELLGMGTI
                     LGFAIPYFSTALWDKWIIMVMPPGAFFALAAVLWICRSIRPKEVKR"
     gene            10506..11105
                     /locus_tag="PLE88_07325"
     CDS             10506..11105
                     /locus_tag="PLE88_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOM76311.1"
                     /translation="MNSAGSEIWLAVVTCFAAVFTNNILLTTFLGMCPFTSVSREVKT
                     AAGLGAAVVFVMTCTTVLNWLAYRYVLVPLQLEFFRYILFIIIIAAFVQFVEMVIDRY
                     LPRLYYNLGIFLPLITVNCAILGASLFMVIRNYSFIVTIGYGFGSGAGWFLAILAMAG
                     IRQRLSEARVPAGLRGPGITLIIAGIMALAFIGFTGVLN"
     gene            11115..12215
                     /locus_tag="PLE88_07330"
     CDS             11115..12215
                     /locus_tag="PLE88_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOM76312.1"
                     /translation="MLEFFLTILIISSIAAGLALLLVLCQRYIVNYGLCRIVINEEKE
                     LAVEGGKPLLSILRDRKIFIPSACGGRGTCGVCKVKVLQGGGPILPTETPFLTKQEQT
                     DRVRLSCQVKVRSDIRIELPRQLLSVQEFVCICSQIEDLTYDMKRFRFELKSPQTIEF
                     TAGQYVQLLCPRYKGSSEEVYRAYSIASDPQQKTQIDLIIRRVPNGICTTWCFDVLRE
                     GDPVQLNGPYGEFCLSRTDAPAVFIAGGSGMAPFVSILHSMRNARCRRSVHYFFGGNT
                     VRDLCLLEQMKEFEHDLEHFSFIPVVAKPAEGENWDGQTGLVTHAVQRSFKDLSTYEG
                     YLCGSPGMIDASIKVLTELGMPADKIYYDKFA"
     gene            12231..12698
                     /locus_tag="PLE88_07335"
     CDS             12231..12698
                     /locus_tag="PLE88_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76313.1"
                     /translation="MKQSPEEKKLEDLLRSTAIVAGGFMGTDPRHPADVIQEDILETE
                     KRGFTPAQLAQRMHELTELAKPYLGCWVETCRGKIRVKTEEFKGALICPWPHPGLFDK
                     RVTAAERLDTGEKIQWTDLNIHLIAEHSFFEGRGAFFRIEPAEIIRILFEGLT"
     gene            12770..12934
                     /locus_tag="PLE88_07340"
     CDS             12770..12934
                     /locus_tag="PLE88_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOM76314.1"
                     /translation="MEKYKCLLCGYIYDPEAGDPDNGIEPGTAFEDLPDDWVCPECGA
                     GKDDFELLEE"
     gene            13036..14574
                     /gene="zwf"
                     /locus_tag="PLE88_07345"
     CDS             13036..14574
                     /gene="zwf"
                     /locus_tag="PLE88_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOM76315.1"
                     /translation="MKKIEATVQKQDLLCIDAAAGPAGFVVFGASGDLAFRKLYPSLY
                     ELYRRDLMSRHFYLLGCGRSALSDSMFRDSIKEKLQTMLDNPDYEMLHTFVQHFSFIS
                     GNYADPLFYQNICERISVLEEQFKVSGSRIFYLSVPPSLYEDIVDKISQMRLHCPAAS
                     GRMQNVRLVIEKPFGRDLKSAKHLDEILQRHFHESQIYRMDHYLGKETVQNILIFRFA
                     NSIFEPVWNRNYIENVQITAAEQAGVEHRAGYYDASGALRDMYQNHLLQMLALIAMEP
                     PVSFQADCLRDEKAKLLRSIRPILPADVPSRFIRGQYAAGTIEGQPVVGYRQEKGVPP
                     DSLTETFVAGKLFIDNWRWKGVPFYLRTGKRLRRKLTEIRITFRQVPHSMFQPAQFID
                     MPANVLRFQIQPQEGMYLSLQAKRPGSKICMSTLEMAVDYQQVFGVIMPESYQRLLLD
                     CMTGDQTLFTRRDSIEISWEILTPILDFWSQQKDLNLYPAGSEGPELQKALLEDTGHS
                     WSSI"
     gene            14824..15453
                     /locus_tag="PLE88_07350"
     CDS             14824..15453
                     /locus_tag="PLE88_07350"
                     /inference="COORDINATES: protein motif:HMM:NF003996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOM76316.1"
                     /translation="MNRKCIIRLSRYKNALKRLKALGFVKVFSGNLADAAGVTSSQVR
                     KDFSLFGITGNRRGGYKVDELIARIREILGKNELQKVVIVGVGNIGKALIRYKGFEAG
                     GIKIVGAFDIDPAKYTRSFEVPVLPMEDFRAFVQEHQVKIGILAVPDVAAHHVSEIMI
                     SAGIKGILNFAPIQLRAADDVVISNINLEVELENLIYFVNAQEKTRASE"
     gene            15453..17294
                     /locus_tag="PLE88_07355"
     CDS             15453..17294
                     /locus_tag="PLE88_07355"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOM76317.1"
                     /translation="MKTRSLKKKILSAFTAVIVLLAASIFALGWYVVRKDLFEQTQLR
                     VIRSLDSARLFYEEEINRIGASLQMADLSQSDDLLRDKLRVHYFYRLKPEQAAAADSE
                     IVRAAASSKQPVGGSRIIGREELERINRGILKNVQIEILPTEKARPTTLKKLETAMAK
                     EYALPILNADGQLQQVVYGGRIINQDFYLVDRIRKLVFGDETFNGIPIGTVTIFQDDV
                     RISTNVLDDKGRRAIGTRISAEVYEAVVEQGQRWLDRAFVVKYWYRSAYEPIRNINNV
                     IIGILYVGILEEPYNAMAAQILMMFIIVILLASALAFVLAFILAGSISRPLTVMRDAT
                     QRLSQGELGHLILSPHSDIEEIHQLARAFNDMSVQLEEREVRLQENHQKLTELNKSYL
                     DLLGFVAHELKGLLSSAVINAYSLRDGLLGLINFKQKKAVDSICRNLDYLDATVKKFL
                     NLSRIERGNLEVNKTEFCLRNVFEAAIDTFAKQLGAKRMSLDNKIDAGLKVRADMDLM
                     MIVANNLVNNAIKYGSDGGQIELSSQTSGEMITIEVYNDSRPIAPDMMGQLFKKFSRL
                     NVPEKKSVKGTGLGLYITKQIVEAHGGTIWVQPREHGNSFIFTIHKE"
     assembly_gap    17297..17306
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17307..17661
                     /locus_tag="PLE88_07360"
     CDS             <17307..17661
                     /locus_tag="PLE88_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOM76318.1"
                     /translation="VVTPLEMIRKKRAEAVNRIISKGYLSTKRVYVGMATCEIAAGSK
                     EVMQVFRQAIANGLTDVYLSQKGCAGRCNLEPTVEVVEEGHIPVKYGRVTPERAREII
                     ERHLKKGEIISEWVI"
     gene            17676..>18544
                     /locus_tag="PLE88_07365"
     CDS             17676..>18544
                     /locus_tag="PLE88_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOM76319.1"
                     /translation="MKQIQYKLTLCDGPTCVHNQSKQLRDALESEIDKAGIRKSVSIH
                     LSGCLGMCKQGPILIVNPGYTMYGNVKPADVSEIVQSHLVSGKPVVRLIIREDHLYNR
                     FFRIFGDADFFGKQMRIALRNCGIIDPENIDDYLSLRGYEALAKVLTDMSPEAVIAEV
                     KKSGLRGRGGAGFPTGLKWELTAREKSSEKYVICNADEGDPGAFMDRGSIEGDPHTIL
                     EGMIIGGYAIGSRNGIIYIRAEYPLAIKRLEKAIAEARQHGFLGEHIFGTDFSFDIEI
                     RLGAGAFVCGEETA"
BASE COUNT         4566 a         4111 c         4900 g         4957 t
ORIGIN      
        1 tcctgaattt ggctgtcttg tatctgtatt tgtcggacgg ctccatccag ctgcccctgc
       61 atctgctcta tctgctgctg gtactggttg catgtttttt ccaattcatc ggcttggatg
      121 tcgtaggtct gctgcaattg caggtatgac ccgcgcagct gcttcaaagc gccgaaggcg
      181 tcttccaatt gacgttccag cccggatttt tgaagggata atcgtttggc agcggttttt
      241 tcctgattca gtttttcttg gaggattgtt ttggcttttt cgctttcctg caaaagcagg
      301 cgggcagatt ccgtctcggc agtttggctt tcaagccggc ggtgcagatt gcgaatgtct
      361 ttttgaagcg attccacctg ctcaagggtc tgctggtatt ttatctccgc ctcagcggcc
      421 gcagcaagac gactttggag tgtgctgatt tgcccgcagg cctcatctaa attggccttt
      481 agcgcatcaa tctgcttgtg tgcctcctgc cgctggcatt cagcggcctg tatgcggtga
      541 tggaattctt cctgctgacg gtgcgccgct tggctggtct gccgcagctg atcttcagcg
      601 gcctgcagct tggcttcgag catccggcac tgctgggcaa gttgttcagc aagcgacgta
      661 tagcgctggg cttcttcttc ccactgctgc cggcgattgc ggtagtcccc cagttcagcg
      721 gcggcagctt ccagctgaat atccttttcc ttcacttgtt cagacaactt tagaatctgt
      781 tcttcaaaca gaagacattg agaagcggcg gcttggtatt tttcaccggc gtctttgagc
      841 ccagccagct cattcagcaa ggcagtgttt tcctgttttt gctgttggag ttctgcaagc
      901 agggctgcat tttctgtttt cagctgctgc cgctgggctt cggccagatg gatatgctca
      961 tccttcaacg ccagttccgc atcggttttc tccagctgtt tctgaaggcc ttgaacctca
     1021 caggccagct tctgtttttc ctgctccagc agttcgatat gagactgaag atcgttgacc
     1081 tgatggatct gctgttggcg ggcagcttcc atttcggcca gttttgattg tgccgtccgg
     1141 gatatcatct gttcctgctg gagagcttcc tgaatcgagc agaagtcgct ttggagcaat
     1201 tgcagctggc tgattttgtc ctttaattcg ccgatatact cggcctgatc acggcaggtg
     1261 cctgccaaat tttctttctg ccttaaggcc aaacgcaatt cctgttggac ttgctgaaac
     1321 tgaagctgta aatccgtcaa ggcagcattt ttttcccgtt ctttctggac aagctcttcc
     1381 agagaaacaa tttgaatatc cgcccagcgc agtttctcgc tgagagaagc gatggcgcag
     1441 gcctgttcgg cagagtgtgc ctcaagcagc tggaactgct catcagtctg gtttttctgc
     1501 tgctgcagct gagccagctg atgctgcagt tgcccaatat attcaaattg gtcctgacag
     1561 gtgcccatca ggttttcttt ctgtcgggtc agccgcttga ttgtgcgctg ggactcttcg
     1621 aaactttcct tgacagcagc aagggtggat tgggactgct gaagctgacg taaaagcata
     1681 tctttgggac tgcggcgctg aagcagctca agcactctcg gtcttccctc tgggcatcgg
     1741 ctgtcggaca gctgctctgc aggacattct tctgtcaaca ctttttgctg taccagcggc
     1801 gtaatatctg aactcactca acactcctta atttcccgaa tggatgtttt acaccggata
     1861 caaacggaac gtatccttct atctatattt ttcggctttc gccggatttt cttgttagta
     1921 taatttttga gaaatatacc ggatgccggc aaaaaggttt ttatgggact aaaaatgtaa
     1981 gattgacttc agcagagcag tttacctaaa gcagatgaag ctcccatgca tacttatatg
     2041 ccctttgtaa agaacaagca gctgctatgt aaccggagga tgccttggtt agatctggaa
     2101 atgtcaaaac cgattggcga ttttttaaaa aatattggat gcagcaaaaa cagaacattg
     2161 cggttaacct ttttattatt aaagccgcct ctaagttctt ttcggcgtag gtacggccaa
     2221 ttctgccctg tgctgccgcg tgcccatcct gatagggatt gctgttttgt ggatattccc
     2281 agaaagacga taccaggcat ctttttttgt aatccgacat ttagtatcct ttttcatcga
     2341 acccactccc ggtatccttt ttcagaaaaa cgatatgcgg tgtcctgttc taagggcggc
     2401 gatgtgtatg ttgggccgtc taataccatc gacaacgctg gaaaattaga gcattatcca
     2461 ctattgtcga taattgttga ttactctctc atactctact cttagagttt atcttccaga
     2521 gctcccatca cctccagcag cagcaacctg ctgccaaaaa agatgcatag tacactctgg
     2581 acattcaaaa tctatttttc ggtttgcaat aattttggta aaatttttgc tgctgcatag
     2641 agtccatcta ttcaaacgat gacactattt cttgaccacc cgaatactgg aataccattg
     2701 acaaacagat tccgattccg tatcctgcgg cagttggctg acagcggggt gtagctcagc
     2761 ttggcttaga gcgcctggtt tgggaccagg aggccgaagg ttcgaatcct ttcaccccga
     2821 tttttcacag atttctaccc ttcgggctga ataatgattg tcaatgaact attctactca
     2881 ttgcaggggg agggccttct ggccggagtg ccgagcgttt ttgttcggct ggcgggctgt
     2941 ccgttgcgat gccgctggtg cgataccaag tatgcttgga atgcggatgc gggacaatca
     3001 atgaccgctg agcaaatact ggaacacatc gcctgctatc ccacccgtta tgttgtcctt
     3061 acagggggcg agccgctgat gcatgaaggc atttttgacc ttattagggc aatccatcgg
     3121 aaggcatacc atttgacagt ggaaacagca ggcattatat ctccggctga gttgccgatc
     3181 gatttgatga gcataagtcc caaattatcc aatacaatag cagatggcac tcaaagccct
     3241 ccagctaatc ccgaaacagt tatgcgactg attgcttctt acccctatca gctgaaattt
     3301 gtagtagatg acccaaaaga tttggatgaa atcgcatgtt tcgtcaataa actggcagca
     3361 gttgatcctt acaagattta tctgatgccg caggcagcca atacggctga ttatctcgaa
     3421 aaatcccgct ggctggctga atactgcctg caaaccggct tcctgttttc accgcggctc
     3481 catattatgc tctggaacgg caggcggggc agatagcggc aataacggct cttttattct
     3541 aataatcaat gataaaattc ttaaaattaa tgaacattat aactgaatag tagtatttat
     3601 taaataagat tagatactat taaggcttca ataatggata aagaggaaga aaaaatcagg
     3661 attgacaaaa aaattgcgga atttaaggat aaatgccgct taaacggttt gcggattacc
     3721 ccccagcgtt tggcgattta caaagcactg gttgaaacaa aaacacatcc gacagccgaa
     3781 gaggtgtaca gtcaggtgca aaaggaattc agtaatattt cactggatac tgtcaacaga
     3841 actctattga cgctttcgga aatcggagca gcttttattg tggaagggac aggacagccc
     3901 agacgctatg acggaggact ggaaaaccat cagcattttc gctgtattaa atgcggtacg
     3961 attatggatt tctactatga accctacgat aaacttgaca ttcctgacta tttgcaggtt
     4021 actgtcctgc gtaaaacggt ctatttggaa ggattgtgcg gcatctgctc gggaatgcct
     4081 gcattccatt aagcaatcac cattttgttt aagaaaggaa aggttagtat gagtttaaaa
     4141 ggaacccaga ccgagaaaaa tctcttgacg gcattttgcg gcgaatcgca ggctcgaaac
     4201 cgctatacct atttcgccag tcaggcaaag aaggaaggtt acgtgcagat ttctgcgata
     4261 tttgaagaaa cagccaatca ggaaaaggaa catgccaagc gtctatttaa gtttcttgaa
     4321 ggaggcgaag tcgaaatttg tgcggctttc ccggcaggcg ttattgggac gacggaggaa
     4381 aatcttttag aggcagccaa tggcgaaaac tatgaaacca cccagatgta tccggatttt
     4441 gcccagacgg cagaaaagga aggttttaag gaaattgcgg cagtattccg caagattgct
     4501 gtcgctgagg cccgtcatcg cgaccggtac caggccctgc gcaacaatat tctaaacgga
     4561 caggttttcc gccgcgacac aaaagtacgc tgggtctgcc gcaattgcgg gtatgtgcat
     4621 gaaggactgg aagctcctag tgtctgtccg gcctgcaatc atccgcagga ttattttgaa
     4681 ttggaagccg ttaactatta accgaggagg gcagcaaaca ccgatgagtc ggtgaccggt
     4741 gcggagggga cagccggctc acccctcatc ggagctgcgg gaatgcggaa ggagcagaaa
     4801 gtgaaaaaaa cacttttgat tattttatgg atttggatta ttttatctgc tggctgtgaa
     4861 aggaacccat ctatggaaag aaaaaatact gttacaatga aaggagcccc cctgaccctg
     4921 acgggtgaat tggtttctgt tggtcagccg gcaccggatt ttactgcagc agccaatgat
     4981 ttatccccgg tcaagctttc ggattacaga ggcaaagttg ttgttattgt gtcggtgccg
     5041 tctctggata cgcccgtctg cgatatcgaa acgcggcggt ttaatcagga agcagccgaa
     5101 tttggagatc aggtggcagt tttagttgtc agcatggatt tgccgtttgc ccagaaaaga
     5161 tggtgcggag cagcaggagt cgggaatgtc cggacggtat ccgattatca atttgccgaa
     5221 tttgggacaa aatacgggct gctgattgaa cagctgcgtc tgcttgcccg ggctgtggtt
     5281 gtagtggata aactcggtac ggttcagtat gttcagctgg tccccgaaat agcttctgaa
     5341 cctgattatg acgatgtcag agcggccata aaaaaaactt ctttgagcaa atgaacgaaa
     5401 ttgcaacatt catgtttgtg aggcaattgc tatgaaaatg cttgctgaca atgtcttagc
     5461 ggtcggcgtt cagcactggg accgccggtt gtttgatgaa ctgattcctc tgcctgacgg
     5521 caccagttac aacgcctatc tggttaaagg aagccagaag acggcgcttc tggacacggt
     5581 ggatcctgaa aaaatagagg ccctgattga ccatttgatg cgggcggggg cagaaaagat
     5641 cgattatatt gtcagccacc acgccgagca ggaccattcc ggcggcatcc cggatatgct
     5701 gatgatgtat ccggaagcca aggttgttgc caatcccaaa tgcaaggcga tgctgatgga
     5761 ccacctgcac attgatgccg atgtattcat cgaagtgcag gatgggcaat ccctttcgct
     5821 gggtgataaa accctgcatt ttctctatac gccgtgggtt cactggcctg agacgatgac
     5881 cacatggctg ccggaagaca aaatcctgtt cagctgcgat ttcttcggat ctcatcaggc
     5941 cgcctccaat ttgtttgttc aggatgaagc ccgcaccttg gaggcagcca aacgctatta
     6001 cggcgaaatt atgatgccgt ttcgcagctc catccgcagc aaccttaaaa aactggagcc
     6061 gctggatatc cgtttgattg ctcccagcca cgggcctgtt taccagaagc ccgagctgat
     6121 tatccatgcc tataaagact ggacggatga ggataaggtt gccaacgccg ttgttcttcc
     6181 gtatgtttcg atgcacggca gtaccggcaa aatggccacc taccttgtgg aggcacttat
     6241 ggagcggggc attgatgttc atccgttcaa tctgtccgct gcggatatag gcgaaatagt
     6301 aatttcgctt gtcagcgcag caacggttgt catggggatt cccactgtgc tgaccggggc
     6361 gcatccatcg gccctgtatt gtgcggcgct ggttaacgcc ctcaggccca aaaccaaatt
     6421 cgtcggcatt atcggttctt acggatgggg cagcaaaatg gttgagcaga tcagcagtct
     6481 cctgggcaat gtaaaggcag aaatgttgga gccggtgctg gccaaaggac aggcccaaga
     6541 ggaaacatac tcgaaattgg atgccttagc cgacacgatt gctgccaagc accggcagct
     6601 ggggatagca tagcaggcgt taataacgaa aaggaatcta ttcagattgg gagtgtaaca
     6661 atggcagcaa aacttgaaat ttacaaatgt atggcatgcg gcaacattgt agaggttgtt
     6721 cacggcggcg atggccagct ggtctgctgc ggccagccga tgaaaaaaat ggtcgaaaac
     6781 acagtggacg cggccaaaga aaagcatgtg ccggtcatcg aaaaaatcga cggcggcttt
     6841 aaagtgacgg ttggtgcggt agctcatcct atggaagaaa agcactatat tgagtggatt
     6901 gaactgcagg ccgacggtca ggtgtatcgg cagtttttaa gccccggcca gaagccggaa
     6961 gccgtgtttt gggtgaaggc cgaaaaggct gctgcccgcg aatactgcaa cttgcacggt
     7021 ctctggaaag cctaagacca acccccggtt gttttagcag attttaagag gagaaaacaa
     7081 tgcttaacaa aaagatggaa gaggcattga ataaacagat taatgcagaa acctattctg
     7141 catatctgta ttggtccatg tcggcagcac tggaaaaaat gaatcttccc ggatttgcca
     7201 gctggatgcg ggtgcaggcg caggaagaga tggttcatgc gtctatgttt tacaagcata
     7261 ttatcgagcg cgacggaacg gtccggctgg cggctgtcga tgcgccgccc catcaatgga
     7321 gcggcgtcca gcaggtattt gaagcagccc tgaagcatga gcagcatgtc agcggcttga
     7381 ttaacagtct gtttgatttg gctgtgcagg aaaaggatca tgcagcaaac ctgtttctgc
     7441 aatggtttgt aaatgagcag attgaagaag aaaagaatgc cagagatata atcggccggc
     7501 tggaaatcgc cggcggcaca gcaggcggat tatacatgct ggacaaagaa atggcggcac
     7561 gtgtgtttac gatgccggcg gccgaacagc aaccttaaga gcagtttgtt tgaataggag
     7621 aatcgaatgg cgattacatt caacgcggat gaaatttttg agatggcaga agaaatcgag
     7681 cgcaacggtg ctgccttcta ccgggaagcg gcagccaaag catccagaga ggacatcaaa
     7741 aaaatgctgc tggatctggc ggcgatggaa gaaggacacg aacaaacttt tgcccagatg
     7801 cgaaagaaat tatctcccgc cgagaaggaa cagacaacgt atgaccccga caatgaagtt
     7861 gcccagtatc tgcggacgat ggcagatttt cacggcaccg agggcaaaat cagtccttca
     7921 gaaaagctta ccggcaatga gagtatgacc gaaatactga agattgctct gcaggcagag
     7981 aaaaattcca tagccttcta tgtggggatt aaggatttgg tgccttccag agccggaaaa
     8041 gggaaaatcc agacgattat aatcgaagaa atggcacatg tctcagccat aggcggaaaa
     8101 ctgcagtcgc tgcgtcaagc cgctgattaa cagcggttgt tattctatct ggatccaagc
     8161 cggctgactt cggggcgtcc agaaagccgg catgttcgac agggtggttt atgtcaaagg
     8221 cagcaggcgg cataggactc atgcgaagcc agccggcgat gaggaagtta ctgctcggga
     8281 cttgtccttg tatcggcggg gccgtgtatt attttggctg gcgttcgctg gcaatgattt
     8341 tgttctgctg tgcggtgggt ttcttctgcg agtggctgtt ctgccgccgc cgaaaagaac
     8401 ccgttaccga agccgtcttt gtaacagcgg ttttgtttgc gctggcgatg ccgcccggcg
     8461 ccggctggca tgtgctggcc gcgggcatgg ttttcgccat tgttgttgcc aaagaggtgt
     8521 tcggaggatt tgggcgaaac atcttcaatc ctgctttggc tggccgatgt tttgtttatg
     8581 tttgttttcc ggttgcgatg acgggcatct ggtatccgcc tgcgcagggc tctttagggg
     8641 cactgctcca gtggaccagc gcccgacagg ccgatggaac gatagccgtt accggggcta
     8701 cacccatggc gcatctgaaa gctggccgcc tggtccgtac tcccgatgcg ccgtatgaag
     8761 ccggcattcc tttccgcatc ggcagtcagg aaacggcggc tgtccgcagc cttgtactct
     8821 acaaggccca gtttttcggc cgcatcagcg gttcgatggg tgttaccagc gccttgctta
     8881 ttttaatagg cgctgttttt ttattttgga ccagaaccgc cagccgcaca acaattctgt
     8941 ctgtagtcct aacatatgct gttttgaacg agctgctgtt tcggctgggc attgctcctg
     9001 ttccgggcgc tgtgccggct gtgctgggag gaggtttttt attgggggcc tgttttatgg
     9061 ctaccgatcc ggtcagttct ccccggaccg aatggggccg cttttattac ggcgttttga
     9121 ttgctgcggc aacggttgtc atccgcaatt tctcgatttt taatggcggg ctgatgtttg
     9181 ctgttttgct gggcaatatg tttgcaccga ttatcgatta cggtgtgcag acattcggcc
     9241 agcgtaaaaa agcaaaacgg attttggagg agaacgccgg atgaaagaca agccgtggtt
     9301 tgctgttgtt tatatgtttg tgatgacagc ttttgccagt tccatcttga ttggtttttc
     9361 ccgcctgacc cgcagccgag tagaagccaa tgcccagctt cagtttgaac gggcggtggt
     9421 gcaggttttt cccgacatac gctttcaaca cgatgagcag atacaccgga tttttaccca
     9481 gcagtttgaa aaagatcctc gcagcggtgc ctttcgcttt ctaaaggacg gccggcttgc
     9541 cggttatgct gtgccgtttt cgggccaggg cttctgggat aaaatcgaag gagtcattgg
     9601 cgtagctgct gacaggcgga cgataacagg aattgctttt taccggcaga atgaaacgcc
     9661 gggtctgggg gcacgcattg aagaagaaga atttcgcagc caatttgccg gaaagagact
     9721 ggccgaaagc ccgatccttt tggaaattgt gccctctgca cagccgcttg ggcccaattc
     9781 tgttcatgcc gtcaccggtg cgacccaaac cagcgatcgg cttcaaattc ttctcaacgc
     9841 caatttacac acatggctgg aggccgtgtc gaatgagggg gaagcccaat gaattctgtc
     9901 aaatcaccgc gggaaattgt gttagatggt ctttggcggg acaatccgat tttccgtcag
     9961 atattgggaa tctgcagcac tctggctgtg accaacgcag tgctcaatac cgcagttatg
    10021 tgtttgtcgc tgacaggggt aacggcttta agctgcttta cggtgtccgt tctgcggaat
    10081 tggacgccgc gaaatatccg aatgatggtg cagacattga tcatcgcttt ttatgtcatt
    10141 attgtggatt tgatgctgag agcttactgg cctcaaatga gccgcaatct cggtcctttt
    10201 gttggcttga ttattaccaa ctgcatcgta atgggccgct gcgaagcatt cgccggctcc
    10261 aatccgccgt tttcctcttt tatcgacggt ttatttaacg gattgggcta ttctatcgtt
    10321 ctgctggtga tttctgtttt tcgcgagctt cttggaatgg gaacaattct tggttttgcg
    10381 attccttatt tttcgacggc tttatgggac aagtggatta ttatggtcat gccgccggga
    10441 gctttttttg ccttggccgc agtcctgtgg atttgccggt ctatcaggcc caaggaggtt
    10501 aaaagatgaa cagcgccggt tcagaaatat ggctggcagt cgtaacctgc tttgcggctg
    10561 tctttaccaa caacattctg ctgactacat ttttgggaat gtgcccgttt accagtgttt
    10621 cccgcgaggt taaaacggct gccggattgg gagcagctgt ggtttttgtg atgacctgta
    10681 caacagtgct gaactggctt gcataccgat atgtccttgt gccgttgcag ttggaatttt
    10741 tccggtatat tttatttatt attattatcg ccgcatttgt gcaatttgtg gagatggtta
    10801 ttgaccgcta tttgccccga ttgtattaca atctggggat tttcttgccg ctgattacgg
    10861 tcaattgtgc aattttaggc gcatcgctgt ttatggttat ccggaactat tcttttattg
    10921 taacaatcgg gtatggtttt ggcagcgggg cgggatggtt tctggcgata ttggctatgg
    10981 ccggcattcg tcagcggctt agtgaagcac gggtaccggc tggcctgcgc gggcccggta
    11041 ttacgctgat tatcgccggc attatggctc tggcgtttat cggatttacg ggggttttga
    11101 actgactgca ctctatgctg gagttttttc taaccatttt gattatcagt tcgattgcag
    11161 cagggctggc gctgctgctg gtgttgtgcc agcggtatat tgtcaattac gggctgtgca
    11221 ggattgttat taacgaggaa aaggaattgg ccgttgaagg gggaaagccg cttttatcaa
    11281 tcctgcggga cagaaagata ttcatcccca gtgcctgcgg aggccgcggg acctgcggtg
    11341 tctgcaaagt caaggtattg cagggcggag gtcctatcct gccgactgag acgccgttct
    11401 tgacaaaaca ggagcagacc gatcgggtgc gtctgtcttg tcaagtcaag gtgcgcagtg
    11461 atatccgaat cgagctgccg cggcagttat taagtgttca ggaatttgtc tgcatatgca
    11521 gtcaaattga agacttgacc tacgatatga agcgatttcg atttgaactg aaaagccccc
    11581 aaacgataga atttacagcc ggtcaatatg ttcagcttct gtgtcctcgt tataaaggca
    11641 gcagtgaaga agtgtatcgt gcctattcaa tagcttctga tccgcagcag aagacgcaga
    11701 ttgatcttat tattcggcgt gttcccaacg gtatttgcac gacttggtgt tttgatgttc
    11761 tccgtgaagg tgatccggtg cagcttaacg gcccctatgg tgaattttgc ctgagccgta
    11821 cggatgctcc ggcggttttt attgccggcg gcagcggaat ggctccgttt gtttctattc
    11881 tgcattccat gcggaatgcc cgctgccgcc ggtcagtgca ttattttttc ggcggcaaca
    11941 cagtccggga tttatgcctt ctggagcaaa tgaaagagtt tgagcacgat ttggagcatt
    12001 tttcatttat ccccgtcgtg gcaaaaccgg cagaagggga aaactgggac ggccagacgg
    12061 gtttggtaac acacgctgtc caaagaagct ttaaggattt aagcacttat gaaggctatt
    12121 tgtgcggcag tcccggcatg atagatgctt ccatcaaagt tttgactgag ctgggaatgc
    12181 cggctgataa aatttattat gacaaattcg cctgatacag gagaccccca atgaaacagt
    12241 ctccagaaga aaagaaactc gaagacctgc ttcgctcaac agccatcgtc gccggcggtt
    12301 ttatgggaac agacccgcgt catccggctg atgttattca ggaagatatc ttggaaacag
    12361 agaaacgcgg ttttaccccg gcccagctgg cccagcggat gcacgaactg acagaattgg
    12421 ccaaacctta tctgggctgc tgggttgaaa cctgcagggg taaaatccgt gtcaagacag
    12481 aggagtttaa aggtgccctc atttgtcctt ggccgcaccc ggggttgttt gacaaacgcg
    12541 tcactgccgc tgaacgcttg gacactggag agaagattca atggacggat ttgaatattc
    12601 atctgattgc cgaacacagt ttttttgagg ggcgaggggc ttttttcaga attgaacctg
    12661 cagaaattat tcgtatttta tttgagggct tgacctgact gcccaaccgg cgataataag
    12721 gccggtataa aaaatttaca ctggaacctc gtttgaaagg aataggacga tggaaaaata
    12781 caaatgtctc ttgtgcggct atatttatga tcctgaggcg ggagatcccg acaatggcat
    12841 cgagccggga acagcgtttg aagacctgcc ggatgactgg gtgtgccccg aatgcggtgc
    12901 cggcaaagac gattttgaac tgttagaaga gtagaccccg ggggccttat ccttggacgg
    12961 attggcgggg tgaaatatgc agctgttctg gaattatggc tgtagtaccc cgcagcattt
    13021 agaacggtct gggctatgaa aaaaatcgag gcgacggttc aaaaacagga tttgctctgt
    13081 atcgatgcgg cagctggtcc tgcgggattt gtggtttttg gcgcatcggg tgacttggct
    13141 tttcgaaaac tatatcccag tttgtatgaa ctttaccggc gtgacctcat gagccgccat
    13201 ttttacctgc tcggatgcgg acgcagtgct ctaagcgatt caatgtttcg agacagtata
    13261 aaagagaaac tgcaaacgat gcttgataat cccgattatg aaatgctgca cacttttgtg
    13321 cagcattttt cttttatcag cggcaactat gctgatcctt tgttttatca gaatatctgt
    13381 gagcgtatct ccgtcttaga ggaacagttc aaagtcagcg gaagccgtat tttttacctt
    13441 tctgttccgc catctctata tgaggatatt gtagataaaa ttagtcagat gagattgcac
    13501 tgcccggctg cttccggccg tatgcagaat gtccggctgg ttattgaaaa accgttcggc
    13561 agagacctga aaagcgctaa acatttggat gaaatactcc agcgtcattt tcacgagtct
    13621 caaatctatc ggatggacca ttatttgggt aaagaaacag ttcagaatat ccttattttc
    13681 cgctttgcca acagcatttt tgaaccggtc tggaaccgca attatattga aaatgttcaa
    13741 attactgctg ccgaacaagc cggtgtagaa catcgagccg gctattatga tgccagcgga
    13801 gcgctgcggg atatgtacca gaatcatctc ttgcagatgc tggcattgat agcaatggag
    13861 ccgcctgttt cttttcaggc cgattgtctc agagatgaaa aggcaaagct tctacggtca
    13921 attcggccaa ttttgcctgc cgatgtgcca agcaggttca tcaggggcca gtatgcagcc
    13981 ggcacaattg aagggcaacc agttgtcggc taccggcagg aaaagggagt gccccctgat
    14041 tctttgactg aaacatttgt cgccggcaaa ttatttattg ataattggcg gtggaagggg
    14101 gttccttttt atctgcgaac cggcaagcgg cttcgccgaa agctgacgga aatacgcatt
    14161 acattcaggc aagttccgca ttcgatgttc cagccggctc agtttattga tatgcccgcg
    14221 aatgtcctgc gatttcaaat tcagccgcaa gaaggaatgt atttgagtct tcaggccaag
    14281 cggccggggt cgaaaatctg tatgagcaca ttggaaatgg cggtggatta ccagcaggtt
    14341 tttggtgtta ttatgcccga atcgtatcag cggctgttgc tggattgtat gacgggggac
    14401 caaactttgt ttacccgccg tgacagtata gaaatatctt gggaaatctt gactccaata
    14461 ctcgattttt ggagtcaaca aaaagatctt aatctgtatc cggcaggttc agaaggtcct
    14521 gaactgcaga aagcacttct cgaggataca ggacatagtt ggtcatctat ataaagcaaa
    14581 tccgataaag agcttacaat ttaagttatt tatattcctt tttttgggtt ttattgagta
    14641 attcatgtta ttataaaaca gtattttgtt ttttattgtg aaaaaaatat caattttgct
    14701 tgattagttt tttaaaactt atataattta agtcataaaa aaactaatag atttattatt
    14761 gcggtcagag atgttgaaaa aacagactaa agttgtcatt aaaaagaggg aatgtcaatg
    14821 gttatgaatc gcaaatgtat catacgattg tcgcggtata aaaatgccct aaaacggctg
    14881 aaggccttgg gctttgtcaa agtgttttcc ggcaatctgg cggatgcggc tggggttact
    14941 tcctcccaag tgcgaaaaga tttttccctg tttggcatca ctggaaatcg gcgaggcggg
    15001 tataaggtgg atgaattaat agctcgaatc cgagaaattc ttggaaagaa tgagctgcag
    15061 aaagttgtta ttgtcggtgt tgggaatata ggaaaggctc tgatacgtta taagggattt
    15121 gaagcggggg gcattaaaat tgtgggggcc ttcgatatag atcctgccaa atatacacgt
    15181 tcgtttgaag tgccggtgct gccgatggag gattttcgtg cctttgtgca ggagcaccaa
    15241 gtcaaaatcg gcattttggc ggttcccgat gtggctgctc accatgtttc agaaattatg
    15301 atttcagcag gcataaaggg tattttgaac tttgctccaa tccagctgag ggcggctgat
    15361 gatgttgtta tcagcaatat caatctggag gtcgaattgg aaaacctgat ttattttgtt
    15421 aatgcacagg aaaagacaag ggcttccgag taatgaaaac ccgttcgctc aaaaagaaaa
    15481 ttctttcggc tttcacagca gttattgtac tgctggcagc gtctattttt gcgctgggct
    15541 ggtatgttgt tcgcaaagat ctttttgagc agacacagct tcgggtgatt cgatcgctgg
    15601 acagcgcacg tcttttttac gaagaggaaa tcaaccgtat cggtgcatcg cttcagatgg
    15661 cagatttaag ccagtccgac gatctactcc gggataagct ccgcgtgcat tatttttacc
    15721 gtctgaaacc tgaacaggcc gcagccgcag acagcgaaat cgtccgtgca gctgcttcgt
    15781 ctaagcagcc tgttggaggc agccgcataa tcggcaggga ggaattagaa cggattaacc
    15841 gtgggatact taaaaatgtg cagattgaga tactgcctac agaaaaggca cgtccgacca
    15901 cattaaaaaa gctggaaact gctatggcca aggaatacgc gctgccgatt cttaatgccg
    15961 acgggcagct tcagcaagtg gtttacggcg gccgtattat caatcaagac ttctatctgg
    16021 ttgaccgcat tcgcaagctc gtatttgggg acgaaacttt taacggaatt cccattggta
    16081 cggtaacaat tttccaggac gatgtccgca tttcaaccaa tgttttagat gataaaggcc
    16141 gacgcgctat cggaacccgt atttctgcag aagtctatga agccgttgtt gagcagggac
    16201 aacgctggct ggaccgtgct tttgtggtca aatactggta tcggtctgct tatgaaccga
    16261 tacgaaatat caacaatgta attattggta ttctgtatgt cggcatcctc gaagaaccct
    16321 acaatgcaat ggctgcccaa atactgatga tgtttataat tgtcatcctg ctggcatcgg
    16381 cactggcttt tgttttagcg ttcatattgg ccggctcgat ttccagacct ctgacagtta
    16441 tgcgggatgc aacccagcgg ctttcgcagg gggagttggg gcatttgatt ctgtctcctc
    16501 attccgatat agaggaaatc caccaattgg ctcgtgcgtt taatgatatg tcagttcagc
    16561 tggaggagcg ggaagtacgt ctgcaggaaa atcaccagaa actgaccgaa ctcaacaaaa
    16621 gttatcttga tctgctcggt tttgtagcgc atgagcttaa agggcttttg tcatcggcgg
    16681 ttatcaatgc ctattctctg cgggatggtc tgctgggttt gattaacttc aagcagaaaa
    16741 aagcagtgga ttccatctgc cggaacctgg attatctgga cgcaacagta aagaaatttc
    16801 taaatctgag ccgaattgaa cgcggaaatc tggaggttaa caaaacagaa ttttgccttc
    16861 gcaacgtgtt tgaagcagca atcgatacct ttgccaaaca gctgggagcc aaaaggatgt
    16921 cgctggataa caaaatcgac gcaggactga aggtgcgggc agatatggat ttaatgatga
    16981 ttgtggccaa taatttggtc aacaatgcca tcaaatacgg ctctgatggg ggacaaattg
    17041 aattaagcag tcaaaccagc ggcgagatga ttacgataga ggtatataat gattcccgcc
    17101 cgatagctcc ggatatgatg ggtcaattat tcaaaaagtt ttcacggctc aacgtaccgg
    17161 aaaaaaagag cgtaaaaggg acgggtttgg ggctgtatat taccaagcag attgtagaag
    17221 ctcacggcgg tactatttgg gtacagccgc gggagcatgg aaattcattt atatttacga
    17281 ttcataagga gtgacannnn nnnnnncgtg gttacaccat tggaaatgat tcgaaaaaaa
    17341 cgcgctgagg cagtcaaccg catcatcagc aaaggttatc tgtccaccaa gcgggtttat
    17401 gtgggtatgg caacctgtga gattgccgcc ggttccaaag aagtgatgca ggtttttcga
    17461 caggccatag ccaacggcct gacagatgtg tacctcagcc agaaggggtg tgccggccga
    17521 tgcaatcttg aaccgactgt cgaagttgtt gaagagggtc atattcctgt taagtatgga
    17581 agggtgacac ccgagcgggc gcgggagatt atagaacggc atcttaaaaa aggcgagatt
    17641 atcagtgaat gggtcatctg agaaaggaaa tcaacgtgaa gcagattcag tataaattga
    17701 cgttatgcga cggcccaacc tgcgttcata accagtccaa acagctgcgg gatgcgctgg
    17761 aatccgaaat cgacaaagcc ggcatccgga agtctgtatc gattcatttg agcggctgtc
    17821 ttggaatgtg caagcagggg ccgattttga ttgtaaatcc cggctatacg atgtacggca
    17881 atgtaaagcc ggcggatgta tcggaaattg tccaatcgca tttggtcagc ggcaaaccag
    17941 ttgtgcgtct gattatccgg gaagatcatt tgtacaatcg gtttttccgt atttttggag
    18001 atgcagactt tttcggcaag cagatgcgga tagccctgcg caactgcggc attattgatc
    18061 cggaaaatat tgatgactat ctgtcgctgc gcggctacga ggccttggcc aaagtcttga
    18121 cggatatgtc tcccgaagct gttattgcgg aagtcaaaaa aagcggcctt cggggccggg
    18181 gcggtgccgg ttttcccaca ggactgaaat gggagctcac agcgcgggaa aaaagcagcg
    18241 aaaaatatgt catctgcaat gccgatgaag gagatcccgg ggcctttatg gaccgcggat
    18301 ctattgaagg tgatccgcat actattttgg aagggatgat tatcggcgga tatgctatcg
    18361 gctccagaaa cggcattatc tacatccgcg cggaatatcc gctggccatc aagcgtctgg
    18421 aaaaagcgat tgcagaggcc cgccagcacg gctttctggg cgagcatatt ttcggcacgg
    18481 atttctcgtt tgacattgaa atccggctgg gtgccggggc tttcgtttgc ggcgaagaga
    18541 ctgc
//
LOCUS       DAOTBO010000052        18336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2524_length_18336_cov_5.152508, whole genome shotgun sequence.
ACCESSION   DAOTBO010000052 DAOTBO010000000
VERSION     DAOTBO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2524_length_18336_cov_5.152508"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLE88_07370"
     CDS             <1..220
                     /locus_tag="PLE88_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76320.1"
                     /translation="ITLAGLIVGSWVNLQRQLDMVSHDVKQLLAAQDEVGRKLEQVQE
                     RSIAHEYRLQAVERKMRDSEHFVQGGGR"
     gene            371..1003
                     /locus_tag="PLE88_07375"
     CDS             371..1003
                     /locus_tag="PLE88_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76321.1"
                     /translation="MDHMGAICLQITADSGQKKESTKGIMALSAAVLLLAGCGLRYAA
                     TEQQKENAWLHWRVCRQAAETAAEENSSSPLCGLTALAEAQSEAFVLDYGLPAWRPEE
                     DNSAALLDEAKAAAAQAQADAAKRPDVWAAADGLLELAIGLAGLVGGVYGARAAGFLK
                     TARAKSQALQEIIAGNELFKRLYPDQAGRFKEAQAHQSASTRQIVASEKH"
     gene            1141..1806
                     /locus_tag="PLE88_07380"
     CDS             1141..1806
                     /locus_tag="PLE88_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76322.1"
                     /translation="MNRMVCIAVVAAAFVLCSVSVQAGQGPGRSRRGGAVQTNPKPAP
                     GADTQEQANKAAEEKARKAAAEEARRQDAERKARREAAEAKGAAKSDEAKAEKQARAE
                     TDEAVQKAKKEKAEKAAGQEAESAGSGKGKGKPEAAGQGKGKGQQAEALGKQFVHEQQ
                     KHQQRQARLERMLELAKEKGDAKTIERIESLMAKEQSRYDSKLGRMMEKDARIGQEAA
                     AEE"
     gene            1867..2073
                     /locus_tag="PLE88_07385"
     CDS             1867..2073
                     /locus_tag="PLE88_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76323.1"
                     /translation="MMRVLLLTAVLLLAASGCGRKEQPPAEPTVKTAEEFKAEAQKEI
                     TAENLSAELDKMEKEVEADMAVEP"
     gene            complement(2175..2939)
                     /locus_tag="PLE88_07390"
     CDS             complement(2175..2939)
                     /locus_tag="PLE88_07390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOM76324.1"
                     /translation="MKNFLHDMILQAGAVTLAHKARLAEVAVHRKSDKDLVTEADIAV
                     EQFLIEKIKSRWPHHAILGEESGHSAGSEYRWVIDPIDGTTSFFHNLPFYSISIALQR
                     QNQTVLAAVYAPVLGELFIAEKDNGACLNDAPIRVSGRTVLSDCVMATGFACLRANLK
                     DNNLPLINRIAPKLRGLRILGSAALDLCYVACGRLDGSWEINLNLYDIAAGMLIAQEA
                     GAIVSDFSGGSDKIPFQIAAASPAIYPQLQPLLQGP"
     gene            3034..4536
                     /locus_tag="PLE88_07395"
     CDS             3034..4536
                     /locus_tag="PLE88_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76325.1"
                     /translation="MTIRRADVTSSRAGGTDRQPLKASLRHRAPQSRRWLRWYLGRYL
                     NYRVPAVRVCAGHSSPMDYLWHAYSADGWRRQPDSGQGCSGDCIVWANRGGGKTQLAA
                     AATLLEGLFKPHCRTLILAGSRQQSGRMYDYLCEFVQRGFSDYLDGRMLADGCRFVNG
                     ATVQVLAQSAASVRGLHVHKLRCDEVELFDPEVLAAANFVTHSSRQLTAAMELLSTMH
                     RPYGPMQTLIDTAAGRRIPVFRWCVWEVIEQCRPERSCSRCPLNSDCGGKARQARGYL
                     KIDDVISQMRRSSRAVFESEMLCLRPNLEKAVFAEFNPAVHVRPAGYDAGLPLYRSLD
                     FGFVNPFVCLWIQTDGSGVVRVIDEYVQSRRLIGEHAQAIRRRTPCEESQVVMTFCDP
                     AGSGCNDVTGTSAVRELKALGFRLKYRASRIYEGIGLIRSALRAGDGQSRLVIDPRCR
                     RLIEALLCYHYPDAASETPCKDGIYDHPIDALRYFFVNYYADSKSLIRPY"
     gene            4809..5483
                     /locus_tag="PLE88_07400"
     CDS             4809..5483
                     /locus_tag="PLE88_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76326.1"
                     /translation="MKMLIAVFIGGAVAAQAIAGLVWSGSLSTPANEGLISGGQTWNS
                     AGEGLTVSWVVSPNEDGSWHYAYTFTNGDGGVLTMRVSHFIISVSDNLTAEDVFSFSG
                     DVEKWTIGTFGPAAGNPGFPQDKTMWGLKLDLQNTQLAAEFDSFRAPMWGDFYAKDGG
                     NPKNYAYNTDFGTAVANPNAYWQTPVDMDGNPLFKVLVPDTIPEPATLLLAGVGMLVL
                     LGKRFR"
     gene            5837..6589
                     /locus_tag="PLE88_07405"
     CDS             5837..6589
                     /locus_tag="PLE88_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76327.1"
                     /translation="MKRWTLFLCVGLVCAPAVKAALVDDFESYQLGKVQAPWTGIFAG
                     TGAAVIGAEENGNQYGSWWGANNGARGMWRPLNENSIANSDTATTVYMRVMTEVATND
                     GSFGLSDVAVPSTWGDFEAQVALINGQLNARNAGSIVPLGMSFTPGQWYNVWMVINNS
                     TDTYDVYVTAAGNATGLAPLADDFVFRNSGAGPVGNDLITFLTLANTRADAQPFRIDD
                     IYITPGVNLAIPEPATLALLAVGVLGLRKKKM"
     gene            6640..7335
                     /locus_tag="PLE88_07410"
     CDS             6640..7335
                     /locus_tag="PLE88_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76328.1"
                     /translation="MKRIGIVLLCAALTAMAQGFTVTNGDFEAGGGSNIPDVTGWYDY
                     STANFWEDAWQTNASWITPNGSNVVVFCSWDSMTGDPLTGSYLYQSIGFSAGQSSLTV
                     SFDWGHPNDTNTGRHDGITVSVWASDGTFVPSDAADIKGAGGVKLLDYASYDHIRAGS
                     YGTVGEIWTQVVTLSLSGANAGEEIFLRFNNYKPLDTADPWPVLDNVVIIPEPATMVL
                     LGLGAAVLRRRGR"
     gene            complement(7483..8703)
                     /gene="lpxB"
                     /locus_tag="PLE88_07415"
     CDS             complement(7483..8703)
                     /gene="lpxB"
                     /locus_tag="PLE88_07415"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOM76329.1"
                     /translation="MTQQTQSRHIFISALEYSAEMHCVHLIAALQQKTAAAVWPGQTP
                     PPQQTLRFAGFGGRQLAEAGCSLLDDTVSRAAMIYNVLGQLGYYRRLIRQAQLYFAHN
                     RPDLVIVCDSPAFNFHIAKAAKKHRIPVLFYVAPQLWAWGPWRIGKLRRCCDKLACIL
                     PFEKDWFAARGIDAQFVSNPLFDPIDTDLEKNFKSYTAYDPAAARIALLPGSREAEIR
                     SLWPAMLTIAAALQKKHPAAVFTACAPDALKRALLEKLKSQSPHADLPVAFAAAGLYE
                     TARQADLALIASGSATLQAAAAGCPMIVMYQSNKWLWHLLARWLVRLRFLSLPNILAG
                     KELVPEFMPYFTSLDPIIARADGLLNTPSRLSSISQALIELVRPMAQRRASDAVADIV
                     LDMLAADTSRPAVG"
     gene            complement(8704..9033)
                     /locus_tag="PLE88_07420"
     CDS             complement(8704..9033)
                     /locus_tag="PLE88_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOM76330.1"
                     /translation="MKTVILLTISNIFMTFAWYGHLRFKDKPLWLVVIASWAIAFFEY
                     CFQVPANRFGHGQFTAAQLKTIQEVITLTVFAAFSILYLHEEFRWNYLVGFIFILLSV
                     FFIFHEW"
     gene            complement(9112..10926)
                     /gene="mnmG"
                     /locus_tag="PLE88_07425"
     CDS             complement(9112..10926)
                     /gene="mnmG"
                     /locus_tag="PLE88_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOM76331.1"
                     /translation="MSGNHSYDIIVVGGGHAGAEAAWAAARMGARTLLLTMSRDTIAK
                     MSCNPAIGGLAKGQIAREIDALGGLMGQAIDAAGIQFRILNRSKGPAVQSPRAQADKY
                     LYKDTVRQYLQNCPNLTIEEGIAADILTAAGAVCGIACTDGRTFTAPVVILTTGTFLR
                     GVMHIGPTQWPGGRLDEPASSELSDCLRRLGFDVQRLKTGTPPRLDGSTIDYDKLQLQ
                     PGDPDPLPFSFLNDRIVRPSVPCWITYTTPRIHTLLRDNLHRAPLYTGQIQSVGPRYC
                     PSIETKIVRFADKQQHQVFLEPEDEKISTIYCNGISTSYPKDVQEEMIRLLPGTEHAR
                     IVHYAYAIEYDYCPARQLKMNLETRRLPGLFLAGQINGTSGYEEAAAQGLLAGINAVL
                     KLGHKDPLILGRDQAYIGVLIDDLLTRDIDEPYRMFTSRAEYRLTLRSDNADQRLTPV
                     GRAVGLVDDPRWSRFEKKQAQMKDFEQYLKSTRRQGYSLWQLVQQPQHPFCQQILQDP
                     DIPDLPREVIEAVLIEARYAGYTARQEKQVAAFRNLENIKLPETLDYSAITHLRFEAK
                     EKLARFRPATLGQAGRIGGITPADLTVIQIYLKKHKSL"
     gene            complement(11169..11858)
                     /gene="nth"
                     /locus_tag="PLE88_07430"
     CDS             complement(11169..11858)
                     /gene="nth"
                     /locus_tag="PLE88_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOM76332.1"
                     /translation="MAKAKQSKLAVDPAQAKERVLKILPILKKTYPDAKIALNWDNPW
                     NLWVAVVLSAQCTDARVNIVTKDLFKKYKGPEDYLKVTQEELEQDIRSTGFFRNKAKN
                     IRAAAKKVLADFGGQMPRTMDELLTLPGTGRKTANCVLANAFGIPAVMCDTHVIRLSR
                     RLGLSRNSDPVKLEFDLMEIVPKTKLGGWTMFSHYLIWHGRTICTARKPDCPHCPIAD
                     CCPSANNPDLW"
     gene            11939..12712
                     /locus_tag="PLE88_07435"
     CDS             11939..12712
                     /locus_tag="PLE88_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOM76333.1"
                     /translation="MAQRTAQAGGVFVGTSGYVYDHWKGTFYPSDLPQGRRFEYYACH
                     FATVELNATFYHLFKDSTFAKWQAQAPAGFVYAVKMWRRITHRKRLHDTAADVQTFLE
                     RAILLQRHLGPVLVQLPPGLHREDGRLEDFLAVCTAAAEALKKPLRFAVEFRHSSWFE
                     EAVYGLLRKYNAALVLPDMPRLYGIRECTTDFIYIRFHGRPQLYQSLYSADVLTDWVR
                     WLEPHLHSGTDVYAYFNNDGDANACKNAAQLHGLLTAKR"
     gene            complement(12833..14668)
                     /locus_tag="PLE88_07440"
     CDS             complement(12833..14668)
                     /locus_tag="PLE88_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOM76334.1"
                     /translation="MNRTQYAGLLTVVWIVFAAAIPAQTLLVQYENQSSAARIEPAQM
                     AGRPGIAVIFEGTDDLHYYASASTAPAPGLELTVWANADGIQFGPAVYPVWSFFNDPA
                     AGKVEVYVGRFTVFVPLVQDILPSRMAEVTVHISGLACTSKLCLTPFTQLIRTAVDFS
                     QAQSWPTVAFEPAPPRPAAQPSLTIPSPDNTPPAGPLAETLADWKEQTVGAGAYGSAG
                     YLLLALLAGLSINIMPCVLPIIPLIIMRLVSQASQSARRRIGLGAAFCAGIVLFFAAF
                     AALSVIVKLSTGTALDMNSLYRSPAGVIGLFILLVFFALGLLDIWTLALPASISAGQS
                     RSAGLAGSVSMGFFAGVLSTPCSGAILGAVLVWAQTQPLYVSSAALILMGIGMALPYA
                     ALAAIPKLLDFVPKPGKWMDIFKKTGGFLLLIIAFKFALAALAKDHLLNVLIYGVIFA
                     FCVWMAGTWVGFSTPKAKKWAVRLTAAAIAAAAGFWLLPPTPPTPIHWQPYDAAIVQK
                     AIEAGRPVLLKFTADWCTNCKIVDKNVYQQPDIAKLVSDKGFAAVLADTTQAAFAASA
                     DMKRIFGEPGNVPVTILLNPKTQTLIKLRGIFSADEFRRLLNEQF"
     gene            15152..16237
                     /locus_tag="PLE88_07445"
     CDS             15152..16237
                     /locus_tag="PLE88_07445"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOM76335.1"
                     /translation="MKTYTIGVIGGDGTGPEVIAEAVKVMDAVSAKFGFKVEKINYDF
                     GGERYKRTGEILPDGAIAELKKLDAILLGAIGHPDVKPGILEKGLLLRMRFELDQYIN
                     LRPVRLFPGVDCPLKDKGPKDIDYVVVRENSGDIYTGSGGLMMKDTPHEVATQTAVYT
                     RFQVDRCLQYAFEYARKKGKKARGVGPDNTLGLVGKTNVLTYVYDLWERAFHEMGDKY
                     YPDIKREYYHVDATCMWMVKSPEWFDVLVTGNLFGDIITDLGAITQGGMGIAAGGNIN
                     PEGVSMFEPIGGSAPKYTGKGVINPLAAICAMQMMLETLGQEQAATAVEQAVRYVTAH
                     KLKSLAAGKMGYSTSQVGDMVAQKVSE"
     gene            16317..>18336
                     /locus_tag="PLE88_07450"
     CDS             16317..>18336
                     /locus_tag="PLE88_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOM76336.1"
                     /translation="MRNSMLHRGILLAGVLCLFSAAWAENLVTNPSFEDSRNNRPVRW
                     RTMVWNGQASLETATIGHTGNASVMIESAGGGDAAWTQTVRVEPNSRYRLSGWIKTEN
                     LQPGSSRGALLNVHDYEGVATPAVSGTTDWTKVECEFVVEMGDSIQINCLFGGWGSAT
                     GKAWYDDITLEKIGTFAYESKTVEAQVSVDASQTFEPISKYIYGQFIEHLGQCIYGGI
                     WAEMLEDRKFYFPVPAERDIWRRTGSGARVLAASPWKVIGPKEAVAMTTDNPLSGKHS
                     VQIGTAAGPAGIYQEELALMQGRSYTGRIVLRGEKAAGPVQVSLVWGDGASQRQTVTI
                     RTLSKSFETVPLKFEAGADADNGRLEIVGSGKGTFVVGAVSLMPADNVEGFRRDTLEL
                     LRQLNAPVYRWPGGNFVSGYDWRDGLGDRDRRPTRTNPAWTGIETNDVGMHEFIALCR
                     LIHTEPMIAVNTGFGDAYSAAAEVEYANGDARTPMGRLRAQNGSKEPFAVKWWCVGNE
                     MFGNWQLGYMQLNHYVLKHNWVEEKMRQVDPTIKTVASGEIGGGWTEGMLRSCADHMD
                     LISEHFYCQNRNDITAHVRQIPDSIKNKCDAHRRFRQTIPGLKDKDIRIAMDEWNYWY
                     GPHVFGELGTRYFMKDALGIAAGLHEYFRNSDICFMANYAQTVNVIGCI"
BASE COUNT         3973 a         4492 c         6032 g         3839 t
ORIGIN      
        1 gataaccttg gccgggctga ttgtgggcag ctgggtcaat ctgcagcggc agctggatat
       61 ggtcagccac gatgtcaagc agcttctggc agcgcaggat gaggtcggcc ggaagctgga
      121 gcaggtgcag gaaagaagca tcgcccacga ataccggctg caggcggtcg agaggaaaat
      181 gcgggattcc gaacacttcg tacaaggcgg cggccggtaa gacaccggca aggcaaacgg
      241 taaagacagg caaaaagaca gccggataca acggacatag cggagcggat aggaccagcc
      301 cccgcggggt aaccgcgcag ggctgtcatt gggatatttg gcagcaggca ttttcagaaa
      361 ggagcacggt atggatcaca tgggagcaat ctgtttacag attaccgcag acagcgggca
      421 gaaaaaagaa agcacaaaag gcataatggc actttcggcg gcggtgctgc tgttggccgg
      481 ctgcggactg cggtatgcgg cgacggaaca gcagaaagag aatgcgtggc ttcattggcg
      541 cgtctgcagg caggcggcag aaacggcggc cgaggaaaac agctcatcgc cgctgtgcgg
      601 gctgacggcg ctggcagaag cgcaaagcga ggcgtttgtg ctggattacg ggctgccggc
      661 ttggcggccg gaggaagaca acagtgcagc gctgctggac gaagcaaagg ccgcagccgc
      721 ccaagcgcag gcggatgccg ccaagcggcc ggatgtgtgg gcggctgccg atgggcttct
      781 ggaactggcc atcggactgg ccggactggt cggtggggtc tatggggcac gggcggcggg
      841 ctttctcaag acggcgcggg ctaaatcgca ggcgcttcag gaaattattg ccggcaacga
      901 gctgtttaag cggctgtatc cggatcaggc cggccgcttc aaagaggcgc aggcgcatca
      961 gtcggcttcg acacggcaga ttgtggcgtc agaaaagcat tgagcggcag gatggttgaa
     1021 cacggcaaaa attttttttt gttggttcag cgccgccgga gtgtataata ggtgcagtct
     1081 gccggcaggc agcagcaagc cggcagagat tggaaagcgc attcacaagg agactctgtt
     1141 atgaaccgta tggtatgtat tgccgttgtt gctgctgcat ttgttttgtg cagcgtgtcg
     1201 gtgcaggccg gacagggtcc gggccgcagc cgccgcggcg gggcggtgca gacaaatcca
     1261 aagcccgcac cgggggcaga tacgcaggaa caggccaaca aggccgctga agaaaaggcc
     1321 cgaaaagcgg ctgccgaaga agcccgcaga caggacgccg agcgcaaagc ccggcgggaa
     1381 gccgcagagg ccaaaggcgc cgccaagtcc gacgaggcaa aagccgaaaa acaggcacgg
     1441 gcagaaactg acgaggctgt ccagaaagcc aagaaggaaa aagccgaaaa ggctgccggc
     1501 caagaggccg aatcggccgg ctccggcaag ggcaaaggca agcccgaagc cgccggacaa
     1561 ggcaaaggca agggccagca ggccgaagcg ctgggcaagc agtttgtgca tgaacagcag
     1621 aagcaccagc agcgtcaggc gcgtctggag cggatgctcg aactggccaa agaaaaaggc
     1681 gatgccaaga ctatcgagcg gattgaatcg ctgatggcca aagagcagag ccgctatgac
     1741 agcaagctgg gccggatgat ggaaaaggac gcccgcatcg gtcaggaagc ggccgctgag
     1801 gaataaagcg gcaggtgccg gttgtgcttt gaaacggaat gcaggttgga tttgggagac
     1861 aaaaatatga tgcgcgtact gctactgaca gcggttcttc tgctggcggc gtccggctgc
     1921 ggcaggaaag aacagccccc cgccgagccg acggtgaaaa cagccgagga attcaaggct
     1981 gaggcacaga aggaaattac ggcagaaaac ctgtcggccg agctggataa gatggaaaaa
     2041 gaagttgagg cggacatggc ggttgagccg taagttcgca acggagtgca cgaataaggc
     2101 ggcgatgctg tgccggcagt gtgcagcatc gccgtctgtt ttgaaggggt cttttgcggc
     2161 aggcagatag tgtttcaggg gccctgcagc agaggctgca attgggggta gatggcgggg
     2221 ctggcggcgg caatctggaa ggggatttta tcgctgcctc cgctgaaatc ggatacgatt
     2281 gcgccggctt cctgtgcgat gagcattccg gcggcgatat cgtagagatt gagattgatt
     2341 tcccacgagc cgtccaggcg tccgcaggcg acatagcaga ggtccagcgc agccgagccg
     2401 agaatccgca gtccccggag tttgggggcg atgcggttaa tcagcggcag gttattgtct
     2461 ttgagattgg cccgcaggca ggcaaagccg gtggccatga cgcagtcgga caggaccgtc
     2521 cggcctgaca cgcggatggg ggcatcgttg aggcaggcgc cgttgtcttt ttcggcgatg
     2581 aacagttctc ccagcacggg ggcatagacg gcggccagaa cggtctggtt ttgccgctgc
     2641 agggcgatgg agatgctgta gaacggcagg ttgtggaaaa aagaagtggt gccgtcgatg
     2701 gggtcaatga cccagcggta ttcgctgccg gcactgtggc cgctttcttc gccgagaatg
     2761 gcgtggtgcg gccagcggct tttaatcttt tcgataagaa actgttccac agcaatatcg
     2821 gcttcggtaa cgaggtcttt gtcgcttttg cggtgcacag cgacctcggc cagccgggct
     2881 ttatgcgcca gcgtgacagc tccggcctga agaatcatat cgtgcaagaa gtttttcatt
     2941 gttgatttct gcatcaaggt ttagttcacc agttcccttt taaccggtgc aggataccgg
     3001 aaagggacag aataagcaag gaggaatata gcgatgacga tacgcagggc agacgtgaca
     3061 agcagccgag caggcggaac agaccggcag ccgttgaagg cctctttgag gcatcgcgct
     3121 ccgcagagcc gccgctggct tcggtggtat cttggcaggt atctgaatta ccgggttccg
     3181 gcggtgcggg tatgtgccgg acacagcagc ccgatggatt atctgtggca tgcctacagt
     3241 gcggatggat ggcgccggca gccggacagc gggcagggct gcagcgggga ttgtattgtg
     3301 tgggccaacc ggggcggcgg caagacgcag ctggcggctg cggcgacgct gctggaaggg
     3361 ctgtttaagc cgcactgccg gacgctgatt ctggccggtt cgcgccagca gtcggggcgg
     3421 atgtatgact acctgtgcga gtttgtgcag cggggctttt cggattatct ggacgggcgg
     3481 atgctggcgg acggatgccg gtttgtcaac ggcgcaacgg ttcaggttct ggcccagtcg
     3541 gccgcgtcgg tgcgcggtct gcatgtgcat aagctccgct gcgatgaggt ggagctgttt
     3601 gatccggagg ttttggctgc cgccaacttt gtaacacaca gcagccggca gctgacggcg
     3661 gcgatggagc tgttgagcac gatgcaccgg ccgtacgggc cgatgcagac gctgattgat
     3721 acagcggcag ggcggcggat tccggtgttt cggtggtgtg tgtgggaggt catcgagcag
     3781 tgccggccgg agcggtcgtg cagccggtgt ccgctgaaca gcgactgcgg gggcaaggcc
     3841 cggcaggcgc gggggtattt gaagattgat gatgtcatca gccagatgcg gcggagcagc
     3901 cgggctgttt ttgaatcgga aatgctgtgt ctgcgtccga atctggaaaa agcggtgttt
     3961 gccgagttca atccggcggt gcatgtgcgg ccggcgggct acgatgccgg cctgccgctg
     4021 taccggtcgc tggatttcgg gtttgtcaat ccgtttgtgt gtttgtggat tcagaccgac
     4081 ggcagcggcg ttgtgcgggt gattgatgag tatgtgcagt cccgccgatt gattggcgag
     4141 catgcgcagg ccatccggcg gcggacgccc tgtgaggagt cgcaggtggt gatgaccttt
     4201 tgcgatccgg cgggatcggg ctgcaatgat gtgacgggca ccagtgcagt cagggaactc
     4261 aaggcgctgg gctttcggct gaagtatcgg gccagccgga tttatgaagg aatcggtttg
     4321 attcgctcgg cgctgcgggc cggcgacggg caaagccgtc tggtgattga cccccgctgc
     4381 cggcggctga ttgaggcgct gctgtgctac cattacccgg atgcggcttc ggagacgccg
     4441 tgcaaggatg ggatttatga ccatccaatt gatgcgctgc ggtacttttt tgtgaattat
     4501 tatgcggaca gcaaatcgct gattcggccg tattagggga tggaatcttt caaaaaatga
     4561 taggcatagg agacaattga acaattcaat aaaagtttgc agctgcaagt ttgcaagtgc
     4621 ttaataataa agatgttaca gtttttaaac ggaacgcggt caggaggctg ccgacaacga
     4681 aaattaagaa atgcgttgtt tttgctgcgg gttttgtgta taataataga caccccttag
     4741 aaggggttgg agtttgcggc aggttaacct gccgtcggtc gaaacaattt ttttggagag
     4801 gaagaaagat gaagatgtta attgcggtat tcataggagg tgctgtcgct gcacaggcga
     4861 tagcgggatt ggtctggagc ggatccttgt caacaccagc caatgaaggt ctcatttccg
     4921 ggggtcagac gtggaattcg gcaggcgaag gattaaccgt gtcgtgggtg gtcagcccca
     4981 atgaagacgg gagctggcat tacgcgtaca cgttcaccaa cggcgacggg ggtgttctga
     5041 cgatgcgggt gagtcatttc attatttctg tgtcggataa tctgacggcc gaagatgtat
     5101 tcagcttcag cggcgatgtt gaaaaatgga ccatcggcac cttcgggccg gcggcgggaa
     5161 atcccggatt tccgcaagac aagacgatgt gggggctgaa gctggatttg cagaatactc
     5221 agcttgctgc cgagtttgac agctttcggg ctccgatgtg gggcgatttt tatgccaaag
     5281 acggcggcaa tccaaagaac tacgcctaca atacggattt tggaacagcc gttgcaaatc
     5341 cgaatgccta ttggcagacg ccggtcgata tggacggcaa tccgctgttt aaggtgctgg
     5401 tgccggatac gattcccgag ccggcaactc ttttgctggc gggagtggga atgcttgttt
     5461 tgcttggaaa acggttccgc tgaaaatctt ttgaaccttt caacagattc cggcaagggc
     5521 agccggaatc tgtttttttg cgccgaaaaa gagggaggtt tatggtatcg gggcttgcgg
     5581 gataattatt ttcttttggg cttggcggtt ttttgtaagg cattgaaata aaaggacttg
     5641 aaataaacga cagttattgc tgtgatatgg ctgcataatt tttcttgtac agccggctgg
     5701 tttatgatat agttgtttgt cggagattta cggaggcggt gtgttgagga aagataccca
     5761 tttcatcgtg catgcctcaa caacacagca ttggaagaaa gcgtgtgtgt attttttgtg
     5821 aggaaaggag agaaagatga aacgatggac tctttttctt tgcgtaggat tggtctgcgc
     5881 acctgctgtg aaggcggctc tggtcgatga ttttgaaagc taccaattag ggaaggtgca
     5941 ggcgccgtgg acggggattt ttgccggcac aggggcggct gttatcgggg cggaagaaaa
     6001 cggcaatcag tacggcagct ggtggggtgc caacaacggc gcccggggga tgtggcgtcc
     6061 gctcaacgaa aacagcattg ccaattcgga tacggctacc acggtgtata tgcgtgtgat
     6121 gacggaggtg gcgaccaatg acgggtcgtt tggcctgagt gatgttgcag tgccgagcac
     6181 atggggcgat tttgaggcac aggtagccct tatcaacggc cagctcaatg cccgcaatgc
     6241 cggcagcatt gttcctttgg gaatgagctt cacgccgggg cagtggtaca atgtatggat
     6301 ggtcatcaac aacagcaccg atacctatga tgtgtatgta actgccgccg gcaatgcaac
     6361 aggactggct ccgcttgcgg atgattttgt tttccgcaat tcgggtgcag ggcctgtcgg
     6421 caacgatttg attacgtttc tgacgctggc caacacccgg gccgatgcac agcccttccg
     6481 gatcgatgac atttatatta caccgggagt gaatctggct attcccgaac cggccacgct
     6541 ggcgctgctt gctgtcggag tgctgggatt gcggaaaaag aaaatgtaat cggtgtttga
     6601 ataataatta gacggattta tgtgtggagg aaagacaaga tgaagagaat agggattgtt
     6661 ttactgtgtg cggctctgac agctatggcg cagggcttta cggtcaccaa cggcgatttc
     6721 gaggcaggcg gggggtccaa tatccccgat gtaaccggct ggtatgacta cagcaccgcc
     6781 aatttctggg aagatgcatg gcagaccaac gcctcgtgga tcacgccaaa cggaagcaat
     6841 gtggttgtct tttgttcgtg ggattcgatg accggagatc ccctgacagg ttcgtatctc
     6901 tatcagagca tcggattttc agccgggcag tcttcgctta ccgtcagctt cgattggggg
     6961 catcccaatg acaccaacac aggcaggcat gacggcatca ccgtttctgt ctgggccagc
     7021 gacggcacat ttgttccgag cgatgcggcc gatatcaaag gcgccggcgg tgtcaagctg
     7081 ctggactatg cctcgtatga ccacatccgg gcggggtctt atggtacggt cggcgagatt
     7141 tggacgcaag tggttacgct gagtctttcg ggtgccaatg cgggagagga aatcttcctt
     7201 cgcttcaaca actacaagcc gctggatacg gctgatccgt ggccggtatt ggacaatgtg
     7261 gtgattattc ccgaaccggc tacgatggtt ctgctgggat tgggtgcggc tgtgctgcga
     7321 aggcgcgggc ggtagtctgc cgggccgcag ggaaacatag ctgatttgca cgaaaagacg
     7381 ccgtctgaga aagggcggcg tcttttttgc tgcgccgaca gaaccgccga aacgcacagc
     7441 ggcggcggga gattcctgcc agcggggcgg aaggattggg ggctatccaa cggcgggtcg
     7501 ggaggtgtcg gcggcaagca tatccagaac gatgtcggca acggcatcgg aggcccggcg
     7561 ctgggccatg gggcgaacca gttcgattaa ggcctgactg atgctgctga gccgcgaagg
     7621 ggtattcagc aggccgtcgg cacgggcaat aatgggatcc agtgaggtaa aatacggcat
     7681 aaactccggg accagctctt ttccggccag aatattgggc agcgacagaa accggaggcg
     7741 caccagccag cgtgccagca gatgccagag ccatttattg ctttgataca tcacaatcat
     7801 cgggcagccg gcagcggcgg cctgcagggt ggcgctgccg ctggcaatca gtgccagatc
     7861 ggcctgccgg gcggtctcgt acaagccggc cgctgcaaag gccacaggca ggtctgcgtg
     7921 gggggactgg cttttgagct tttccagcag ggcgcgtttg agagcatcgg gagcacaggc
     7981 ggtaaagacg gctgcggggt gttttttctg taaggcggcg gcgattgtca gcatcgccgg
     8041 ccacagcgag cggatttccg cctcgcgcga gccgggcagc agggcaatgc gggctgctgc
     8101 ggggtcatag gcggtgtagg atttaaaatt tttctcaagg tcggtgtcga tggggtcgaa
     8161 aagcggattg gacacgaatt gggcatcgat gccgcgtgcg gcgaaccaat ccttttcgaa
     8221 cggcaggatg caggccagct tgtcgcagca gcggcggagc ttgccgatcc gccacggccc
     8281 ccaagcccac agctgggggg cgacataaaa cagcaccggg atgcggtgtt ttttggcggc
     8341 tttggcgatg tggaaattga acgccggcga atcgcagaca atcaccagat ccggccggtt
     8401 gtgtgcaaag tacaactgtg cctgccggat gagccggcgg taatatccca gctgacccaa
     8461 gacattgtaa atcatcgctg cccggctgac ggtgtcatcc agaagggagc agccggcttc
     8521 ggccagctgc ctgccgccga aaccggcaaa gcggagtgtc tgctgcgggg gcggagtctg
     8581 ccccggccag acagccgcgg cggtcttctg ctgcagggcg gcaatcagat ggacgcagtg
     8641 catctcggcg ctgtattcca aggcgctgat aaagatgtgc cgggattgtg tctgttgggt
     8701 catttaccac tcgtgaaaga taaagaaaac cgacagcaga ataaagataa agcccaccag
     8761 ataattccat cggaactcct cgtggagata caaaatcgaa aatgccgcaa acaccgtcag
     8821 ggtgatgact tcctgaatgg ttttgagctg ggcggcggta aactggccgt gtccgaagcg
     8881 gttggcgggc acctgaaagc agtattcaaa aaaggcgatt gcccagctgg caatgaccac
     8941 cagccacagg ggtttatcct tgaaccgtaa atgtccgtac cacgcaaagg tcataaagat
     9001 attcgaaatc gtcagcagga taacagtctt cataagttct gtctcttggg atccctttgt
     9061 ttgttcaaac ggcctatcag cataccggcg gaccggccaa aaagcaagcg gctaaaggga
     9121 cttgtgtttt ttgaggtaaa tctggatgac ggtcaggtcg gcaggcgtga tgccgccgat
     9181 gcggccggct tggccgaggg tagcaggacg aaagcgcgcc agtttttctt ttgcctcgaa
     9241 gcgcagatgg gtgatggcgc tgtagtcgag cgtttcgggc agcttgatat tttccagatt
     9301 gcggaaggcg gcgacctgct tttcctgacg ggcggtgtag ccggcgtagc gggcttcaat
     9361 caggacggcc tcgatgactt cccgcggcag gtccgggatg tcggggtctt ggagtatctg
     9421 ctgacagaac ggatgctggg gctgctggac aagctgccag aggctatagc cctgccggcg
     9481 ggtggatttg aggtactgct caaagtcctt catttgtgcc tgcttttttt caaagcggct
     9541 ccagcggggg tcatcgacca gtccgacagc ccgcccgaca ggggtcagcc gctggtcggc
     9601 gttgtcgctt cgcagggtca ggcggtattc agcccgcgag gtgaacatcc ggtagggttc
     9661 gtcaatgtcg cgggtcagga ggtcatcgat cagcacgccg atataggcct gatcgcggcc
     9721 aaggataagc gggtctttat gccccagttt gagcacggca ttgatgccgg ccagcaggcc
     9781 ttgtgcggcg gcctcttcgt agccgctggt gccgttgatt tggccggcca gaaacaatcc
     9841 cggcaggcgt cgggtttcga ggttcatctt tagctgccgg gccgggcagt agtcgtactc
     9901 gatggcgtag gcgtaatgga cgatgcgggc gtgttcagtg cccggcagga ggcgaatcat
     9961 ttcttcctgc acatctttgg gatacgaggt gctgatgccg ttgcagtaaa tggtggagat
    10021 tttttcatct tccggctcca gaaagacctg atgctgctgt ttgtcggcaa agcggacaat
    10081 ttttgtttcg atgctgggac agtagcgcgg gccgacggat tgaatctggc cggtgtagag
    10141 cggggcacgg tgcaggttgt cgcgcaggag tgtgtgaatc cggggggttg tataggtaat
    10201 ccagcagggg acagacgggc ggacaatgcg gtcgttgaga aacgaaaacg gcagggggtc
    10261 ggggtcgccg ggctggagct ggagtttgtc gtagtcgatg gtggagccgt ccaagcgggg
    10321 gggagtgccg gttttgagcc gctggacatc gaagcccagc cggcggaggc agtcggacag
    10381 ttcgctgctg gccggttcgt ccagccggcc gccgggccat tgcgtcgggc cgatgtgcat
    10441 tacgccgcga aggaatgtgc cggttgtcag gataacgacc ggcgctgtga aggtgcggcc
    10501 gtcggtgcag gcgatgccgc agacggcgcc ggctgcggtg aggatgtcgg cggcgattcc
    10561 ttcctcaatt gtcagattgg ggcagttttg cagatactgt cggacggtgt ctttgtagag
    10621 gtatttgtcg gcctgtgcgc ggggactttg cacagcaggg cctttggagc ggttgaggat
    10681 gcggaattgg atgccggcgg catcgatggc ttggcccatc aggccgccga gggcatcgat
    10741 ttcgcgggcg atttgccctt ttgcaaggcc gccaatggcc ggattgcagc tcatcttggc
    10801 gatggtatcg cggctcattg tcagcagcag cgtccgcgca cccatccgcg ccgccgccca
    10861 tgcggcttcg gctccggcat gcccgccgcc gacaacgatt atatcatatg aatgattacc
    10921 cgacatcttt actaccaacc cgctacccaa cgattgcatc atgctgtaat tctatactgt
    10981 tgccctctcc agaagctgcc tgaatactct gctgcctttt ctgagctgca agtgctttgc
    11041 catagcggca tttttttagg ctgtgtttgt tccatgctgc gtattatcgt ttaatgcgga
    11101 atcggccgca aggttttatt ggttaaatcg gcggctgctt cgggctggcg ggatgggggg
    11161 gtgctcggtt accacaggtc gggattattg gccgaggggc agcagtcggc gatggggcaa
    11221 tgggggcagt cgggcttgcg ggcggtgcag attgtgcggc cgtgccagat aaggtaatgg
    11281 gaaaacatcg tccagccgcc gagcttggtt ttagggacga tttccatcag gtcaaactcg
    11341 agcttgaccg gatcgctgtt tcggcttagg cccagacggc ggctcagccg gatgacgtgc
    11401 gtgtcgcaca tgacggcggg gatgccgaag gcgttggcca gaacgcagtt ggcggtcttg
    11461 cggccggtgc cgggcagggt cagcagttca tccattgtcc gcggcatctg gccgccgaaa
    11521 tcggcaagga cttttttggc agcggcgcgg atatttttgg ccttgttgcg gaaaaagccc
    11581 gtcgagcgga tatcctgctc caattcttcc tgcgtgacct tcagataatc ttcgggacct
    11641 ttgtattttt tgaacaggtc cttggtgacg atgttgacgc gggcatcggt gcattgggcc
    11701 gacaggacga cggcgaccca caggttccac gggttgtccc agttaagggc gattttggca
    11761 tcggggtagg tctttttgag aatcggcagg attttcagca cccgctcctt tgcctgcgcc
    11821 ggatcaaccg ccagttttga ctgttttgcc tttgccatag ccgacagtat aaaccggcag
    11881 ggttctgttt gcaagagcgg acttgtggga atgcggccgc cggattagaa tagccggtat
    11941 ggcacaacgg acagcacagg ccggaggcgt ttttgtcggc acatcggggt atgtgtatga
    12001 ccactggaag gggacatttt atccttccga cctgccgcag gggcggcggt ttgaatatta
    12061 tgcttgccat tttgccaccg tcgagctgaa cgccacattt taccatttgt ttaaggacag
    12121 cacatttgcc aaatggcagg cgcaggcgcc ggccggcttt gtctatgcgg tcaagatgtg
    12181 gcggcggatc acgcatcgaa agcgtctgca cgacacagca gccgatgtgc agacctttct
    12241 ggagcgggcg attttgctcc agcggcatct ggggccggtg ctggttcagc ttccgcccgg
    12301 gctgcatcgg gaggatgggc gattggagga ttttttagca gtctgcacgg cggcggcaga
    12361 agcgcttaaa aagccgctgc ggtttgcggt ggagtttcgc cattcaagct ggtttgagga
    12421 ggctgtgtac gggcttttga ggaaatacaa tgctgcactg gtgctgccgg atatgccgcg
    12481 gctgtacggc attcgggaat gcacgaccga ttttatctac atccgctttc acggcaggcc
    12541 gcagttgtat cagagcttgt acagcgcgga tgttctgacc gactgggtgc ggtggcttga
    12601 accgcatctg cacagcggta ccgatgtcta tgcctatttc aataatgacg gcgatgcgaa
    12661 tgcctgcaaa aacgccgccc agctacacgg gcttctgacg gccaagcgct gattatcgaa
    12721 aagcgctgac tgtcaggatc tttggcggga cgggtgtgcc ggcgtgcagg gcagatagac
    12781 cctgttgggg atagagcatc aggctgtttt atgcggggtc taaaagttct ggtcaaaact
    12841 gttcgttcag aaggcggcgg aactcatcgg ccgaaaagat gccgcggagt ttgatgaggg
    12901 tttgggtttt gggattgagc aggatggtta cagggacatt tccgggctcg ccgaagatgc
    12961 gtttcatatc agcggaagcg gcaaaggcgg cctgcgtggt gtcggccagc acggcggcaa
    13021 agcccttgtc gctgacgagt ttggcgatgt cgggctgctg gtaaacattt ttgtccacga
    13081 ttttgcagtt ggtgcaccag tcggcggtga acttcagcag aacaggcctg ccggcttcaa
    13141 tggccttctg cacaatcgcg gcatcatagg gctgccagtg aatcggcgtc ggcggcgtcg
    13201 ggggcagcag ccagaaacct gccgccgcgg caatcgccgc cgccgtcagg cggacagccc
    13261 attttttggc tttgggtgtt gaaaatccca cccatgtgcc ggccatccac acgcagaagg
    13321 caaaaatcac gccgtagata aggacattga gcagatgatc tttggccaga gcggccaagg
    13381 caaatttaaa ggcgataatc agcagcagaa agccgccggt ttttttgaag atgtccatcc
    13441 atttgccggg cttgggaaca aaatcgagca gtttgggtat tgcagccagt gccgcatagg
    13501 gcaaagccat tccgatgccc atcagaatca gtgcagcgct gctgacataa agcggctggg
    13561 tttgtgccca gaccagcacc gcccccagaa tggcgccgct gcagggggtg ctcaaaacgc
    13621 cggcaaaaaa gcccatgctg accgagccgg ccagtcctgc tgaacggctt tgaccagcgc
    13681 taatcgatgc cggcagcgcc agcgtccaga tgtccagaag ccccagtgca aaaaacacca
    13741 gcaggataaa cagcccgatg actcctgccg gactgcggta caggctgttc atgtccagcg
    13801 ctgttcctgt ggacagtttg acaatcaccg acaaagcggc aaaggcggca aagaacagca
    13861 caatccctgc acaaaaggca gcccccaaac cgatgcgccg gcgtgcggat tggctggcct
    13921 gcgaaaccag ccgcataata atcagcggga taatgggcag cacgcagggc ataatattga
    13981 tagacagtcc tgccagcagg gccaacagca gatagcctgc tgaaccgtag gccccggcgc
    14041 cgacggtttg ctctttccag tcggccagag tttcagccag cggaccagct ggcggggtat
    14101 tgtcagggct ggggattgtc aggctcggct gtgctgccgg ccggggcgga gccggttcga
    14161 acgcaactgt cggccacgac tgtgcctgcg aaaaatcgac agcggtgcgg ataagctggg
    14221 taaacggcgt cagacacagc ttgctggtgc aggccaagcc ggaaatgtga acggttactt
    14281 cagccatgcg cgagggcagg atatcctgaa caaggggcac aaagaccgta aaccggccga
    14341 catagacttc cacttttccg gccgccgggt cgttgaagaa gctccagacc ggatacaccg
    14401 caggaccgaa ctgaatgccg tcggcgtttg cccagactgt caattccagc cccggtgctg
    14461 gagctgtaga agcgctggcg tagtaatgca ggtcgtccgt gccttcaaat atgacggcga
    14521 tgcccggccg gccggccatc tgcgccggct caatgcgggc ggcggacgac tggttttcat
    14581 actggactaa taaagtttgg gccggtatgg ctgcagcaaa aacaatccat actaccgtca
    14641 gcagacctgc atattgtgtg cggttcattg tcgtctcctc aatccgggcg aagcgtcagc
    14701 agcggcctca ggctgccggc gctatctgtt tgttttggca atacatacgt tttaccgatt
    14761 tgattgttcc ttcaataaac caattgccgg cagttttgaa aagcaaaaaa tgcatccgat
    14821 tataaagcaa ttctgtatca atattgcccc taaaccgcag ggctatttca aagacagttt
    14881 gacaactatt gagcaagcac aggatttgcc ggataaggtt catgcttccg gcggtttgtt
    14941 ttggcttggg tgcatagata aaaaatccaa ttgtgcttga aacgaacaga gtttggctgt
    15001 aataattgcg gtttatgccg ttttgcgaaa gcatcggcaa ggcaggtctg taatccatag
    15061 atgataaatc taatctgaaa tagagacaaa tctcagattg cagaacagtt gcggatgtat
    15121 cagttactat ctgttgagaa aggataaacc agtgaaaacg tacacaattg gcgtcatcgg
    15181 cggcgatggg acggggccgg aagtcattgc agaagcggtt aaggtgatgg atgcggtcag
    15241 cgcaaaattc ggcttcaaag tggaaaaaat caactatgat ttcggcggcg agcggtataa
    15301 gcggacgggt gaaatcctgc cggacggcgc gattgcggaa ctgaaaaaac tggatgccat
    15361 tctgctgggg gcgatcgggc atccggatgt caagcccggg attctggaga aaggtcttct
    15421 gctgcggatg cggtttgagc tggatcagta tatcaacctg cgtccggtgc ggctgtttcc
    15481 gggggtggat tgtcctttga aggacaaagg tcccaaagat attgattatg tcgttgtacg
    15541 ggaaaactcc ggcgatatct ataccggctc cggcgggctg atgatgaaag atacgccgca
    15601 cgaagtagcg acccagacgg cggtttatac ccggtttcag gtggaccggt gtctgcagta
    15661 tgcgtttgag tatgcccgca agaagggcaa aaaagcccgc ggcgtcgggc cggacaatac
    15721 gctgggtctg gtaggcaaga ccaatgtatt gacctatgtg tatgatttgt gggaaagggc
    15781 cttccacgag atgggggaca agtattatcc ggacattaag cgggaatact accatgtgga
    15841 tgccacctgt atgtggatgg tcaagagtcc cgagtggttt gatgtgctgg tgaccggcaa
    15901 cctgttcggg gatatcatca ccgacctcgg cgctatcacg cagggcggaa tgggcatagc
    15961 agccggcggc aatatcaatc ccgaaggcgt ttcgatgttt gaacctatcg gcggcagcgc
    16021 tcccaagtac acgggcaaag gggtcatcaa tccgctggcg gctatctgtg cgatgcagat
    16081 gatgctggaa acactggggc aggaacaagc cgccactgca gtggagcagg cggttcgcta
    16141 tgtaacggcc cataagctca aatcgctggc ggccggaaag atgggctatt cgaccagtca
    16201 ggtcggcgat atggtcgccc agaaagtatc cgaatagaac gatggcacac caggcagatc
    16261 ctgacaggcg gggtctgccg gtgtttgaaa atgcacttgt tgtcagggag gttattatga
    16321 gaaatagcat gctgcacagg ggaattcttc tggctggagt tttgtgtctt ttttctgctg
    16381 cttgggcgga gaatctggtg accaatccgt cgtttgaaga cagccgcaat aaccgcccgg
    16441 ttcgctggcg gacgatggtc tggaatggtc aggcgtcgct ggagacggcc acgatcggcc
    16501 acaccggcaa tgccagcgta atgattgaat ctgccggcgg cggcgatgcc gcctggacgc
    16561 agacggtacg ggtcgaaccc aacagccggt accggctcag cggctggatt aagacggaaa
    16621 acctgcagcc gggcagttcc cgcggagcgc tgctgaatgt gcacgattac gaaggcgtcg
    16681 cgacgccggc ggtcagcggc acgacggact ggacgaaggt ggaatgcgaa tttgtggtcg
    16741 aaatgggcga ctcgatccag attaactgcc tattcggcgg ctgggggtcc gcgacgggca
    16801 aggcgtggta tgatgacatc acgctggaaa aaatcgggac ctttgcctac gaatccaaga
    16861 cagtagaggc acaggtgtct gtggatgcct cgcagacctt tgagccgatt tccaagtata
    16921 tttacggcca gtttattgag catctggggc agtgcatata cggcggcatt tgggcggaaa
    16981 tgctggaaga ccgcaagttc tacttcccgg tgccggccga acgggatatc tggcgccgca
    17041 cgggatccgg agcgcgggtg ctggcggctt cgccatggaa ggttatcggc ccgaaggaag
    17101 cggttgcgat gaccacggac aatccgctgt cgggcaagca cagtgtgcag attggcacgg
    17161 cagccggccc tgcgggcatt tatcaggaag agctggcgct gatgcagggg cgttcctata
    17221 caggacggat tgtgctgcgg ggcgaaaagg ccgccggacc tgtacaggtc agcttggtgt
    17281 ggggcgacgg ggcttcccag cgtcagacgg taacgatacg cacactgtcc aaatcattcg
    17341 aaaccgtgcc gctgaagttt gaagccggag cggatgccga caacgggcgg ctggaaattg
    17401 tcggaagcgg gaaagggaca tttgtggtcg gggcggtttc gctgatgccg gctgacaatg
    17461 ttgagggctt ccgcagggat acactggagc tgctgcggca gctgaatgcg ccggtgtatc
    17521 gctggccggg cggcaatttt gtcagcggct atgactggcg ggatggtctg ggcgaccgcg
    17581 accggcggcc cacacggacc aatccggcct ggacaggcat cgaaacaaac gatgtgggga
    17641 tgcatgagtt tatcgctctg tgccgtctga ttcataccga gccgatgatt gccgtcaata
    17701 ccggattcgg cgatgcgtat tcggcggctg cggaagtgga atatgccaac ggcgatgccc
    17761 gcaccccgat gggcaggctg cgggcgcaga acggcagcaa agagccgttt gccgtcaaat
    17821 ggtggtgcgt gggcaatgag atgttcggca attggcagct gggctatatg cagctgaacc
    17881 actatgtcct caagcacaac tgggtggaag aaaagatgcg tcaggttgac ccgaccatca
    17941 agacggtggc cagcggcgaa atcggcggcg gctggaccga aggaatgctg cgcagctgtg
    18001 ccgaccatat ggatttaatc agcgagcatt tctactgcca gaaccgcaac gatattacag
    18061 cgcatgtgcg gcagataccc gattccatca agaacaaatg cgatgcgcac cgccgtttcc
    18121 gccaaaccat tccggggctg aaagacaagg atatccgcat tgcgatggac gagtggaatt
    18181 actggtacgg gccgcatgtc ttcggggaac tggggacgcg ctactttatg aaagatgcac
    18241 tgggcattgc cgccggtctg cacgagtatt tccgcaattc ggatatctgc tttatggcca
    18301 actatgccca gacggtcaat gtgatcggct gcatca
//
LOCUS       DAOTBO010000053        18185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2543_length_18185_cov_5.750083, whole genome shotgun sequence.
ACCESSION   DAOTBO010000053 DAOTBO010000000
VERSION     DAOTBO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18185
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_18185_cov_5.750083"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLE88_07455"
     CDS             <1..549
                     /locus_tag="PLE88_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76337.1"
                     /translation="IVLDVFAKLRQDSRLTALRLAIIPRKPERFDEVAALIEQSGFDY
                     VRYSVLKEQDSQAAGKPAVVLGDTMGDLKKFYALAAVVFVGRSLVPMGGSDMMEPAAL
                     GKCTIFGPHTFNFKQTVEVLLEGGGAVEAADEKALYEKTLQCLTDAAYASAIAAEGQA
                     IIRQNQGATKKTLETINRLLKQ"
     gene            647..1045
                     /locus_tag="PLE88_07460"
     CDS             647..1045
                     /locus_tag="PLE88_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HOM76338.1"
                     /translation="MTSQWAAGNDFAELLERLEKSAFRSRFKLGSKERAYLKEKGFTV
                     IQQHARDFIASRLAPAEIANDGKQTPMRGHPVFIAQHATATCCRKCLEKWHRLPQGRE
                     LTSEEVDRIVSILLIWLKRQAQAPARTELF"
     gene            complement(1073..1972)
                     /locus_tag="PLE88_07465"
     CDS             complement(1073..1972)
                     /locus_tag="PLE88_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOM76339.1"
                     /translation="MRAVKSIFRHAALTAILVFFAASQGCQPQQEKASDAAFIPQWGV
                     CTGLDNTAKLQNAGYDYIELGVQRFLVPQSSDEDFAKNLLEAQSVKAPLYACNSFLPS
                     SLICVGPAADHPAILTYAETAFRRAQAAGIRVIVFGSGSSRRVPDGFEPAQAQRQFVD
                     LLKKMGPIAEKYGVIIAIEPLNKKECNFINTVAEGTAIAREVNHPNIRVLADFYHMAL
                     EDEGPDSIRAAGRLLAHCHIAEKEGRKWPGASGFDFTGYFKALKDIRYRGCISIEGRA
                     DDFDRQLPEALAYMKSQWETAGL"
     gene            complement(2055..2789)
                     /locus_tag="PLE88_07470"
     CDS             complement(2055..2789)
                     /locus_tag="PLE88_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM76340.1"
                     /translation="MAVRNIVNIDENKCNGCGQCVRACAEGAIQMIDGKARLVSEIYC
                     DGLGACLDCCPTGAITIEQRQAEDFDEEAVKKHLAQLAANKTAFQKQTQAAGSVCPGM
                     RVLSFAQEAAAVTGDVSSQLRQWPVQLALVSPTAPYFVNADLMLTADCVPFAMGDFHS
                     SLLGGKAIAIACPKLDDTGRYIQKLADIFTAAKPKSLTVVHMEVPCCSGLRLLAQKAI
                     ALAGIALPIRDITVSLHGRILSDKTI"
     gene            complement(2931..4583)
                     /gene="hcp"
                     /locus_tag="PLE88_07475"
                     /gene_synonym="priS"
     CDS             complement(2931..4583)
                     /gene="hcp"
                     /locus_tag="PLE88_07475"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOM76341.1"
                     /translation="MFCYQCEQTIGGKGCAKNIGVCGKTAETAELQDLLIEAVKGIAM
                     YAYRAGQLGYADRSVDRFVVEALFSTVTNVNFDPVRLEALIAKAGQTLAAAKALYLDA
                     CRKKNIQPQPLSGPALWQVPANRTQMLQQAATVSIAKRLDAQGPDVTGLQELILYGLK
                     GMAAYADHAMILGVEDDKVYAFFHEALDFLTGPSAANVDALVAMAMKVGQINLTVMGM
                     LDQGNTGTYGHPEPTTVRVTPVKGKAIVVSGHDLKDLELLLQQTENKGINVYTHGEML
                     PCLAYPGLKKYKHLVGNYGGAWQDQQKEFEAFPGAILMTTNCIQKPRDSYKDRIFTTG
                     LVAWPEVRHIGKDKDFTPVIQAALAAPGFAEDAETKTITIGFGRNAVMNVAPAVIEAV
                     KAGKIRHFFLIGGCDGAKPGRNYYTEFAQLVPDDCVILTLACGKYRFNKLPFGAIGGI
                     PRLLDIGQCNDAYSAIQIAVALAGAFDCGVNDLPLSMILSWYEQKAVCILLTLLHLGI
                     KNIKLGPSLPAFIGPNVLKVLVEKFNIAPTATPQEDLAQILA"
     gene            complement(4611..5111)
                     /locus_tag="PLE88_07480"
     CDS             complement(4611..5111)
                     /locus_tag="PLE88_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM76342.1"
                     /translation="MRQILIEKMKAVFGSDQKRIDHALAVLNYAESIMAKEQAQPNIV
                     VAAAVLHDIGILSAEQKYHSAAGIYQELEGPPIAKAILESLAFPPADINHICAIVGSH
                     HSGGKIDTPEFRCVWDADWIVNLGDDYASWTQAQKQKIIDSQLRTHAGRRIACRLYLS
                     KNMGKQ"
     gene            complement(5185..5547)
                     /locus_tag="PLE88_07485"
     CDS             complement(5185..5547)
                     /locus_tag="PLE88_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM76343.1"
                     /translation="MNKIEPNLNCLETCFGRPAGLQALIDYANDSIVSKTLLDKPAGT
                     ITLFAFDAGQRLSEHTAPFDAVVQVIDGKGVITINGKDHTVQAGQIIIMPADIPHAVH
                     ADERFKMLLTMIRSRNSA"
     gene            complement(5540..5962)
                     /locus_tag="PLE88_07490"
     CDS             complement(5540..5962)
                     /locus_tag="PLE88_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOM76344.1"
                     /translation="MDIIRLDTDYALRIAAALAGAFENQTSLSARQLSSQCSVPYAIA
                     CKILQKLVKAKVAESIMGAKGGFRLAGPPQQIRFGQVVEAIQGRVCVNRCLLGSFRCP
                     LNKNCPAHPKLAVLQSQIDVFLNELTLKEFVHRENRNE"
     gene            6353..7789
                     /locus_tag="PLE88_07495"
     CDS             6353..7789
                     /locus_tag="PLE88_07495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOM76345.1"
                     /translation="MKRQMILQAAVVLVISFMGMPALAAEAETNGIETLRKTSKAFSE
                     VAKKATPAVVAVKVRTKIEVPSMGFGFGSPFDDEFFERFFGRRFPQTPQEPQERWREG
                     QASGFIISPDGYILTNNHVIDEADEISVVLQDGRTFEKVKLIGADDKTDVAVLKIENI
                     DNLPCVELGDSDDLEIGEWVIAIGNPFGLTETLTVGVVSALGRKLGGEGFQDFIQTDA
                     AINPGNSGGPLLNLDGKVVGINSAIITGDRGYMGIGLAVPINMAKSIKDQLVNTGKVQ
                     RGYLGISMQEISPDMAEFFKLKDSKGALITDVVKDSPAERAGLEKDDVIIQIGSKKIE
                     DVQDVRNVIGFTPPDTIVEMTIIRNGKEKKLNVKVGSQEESELADTSETGKQLGLEVA
                     AIDEEKARQYKAAVGKGVVITQVKSGSAAAFAGLREGMIIYEVNRVSVNTVSEFNQAL
                     KESEKTKKVLLLVKVGRYTQYVVLSLKP"
     gene            complement(7858..8382)
                     /gene="pyrE"
                     /locus_tag="PLE88_07500"
     CDS             complement(7858..8382)
                     /gene="pyrE"
                     /locus_tag="PLE88_07500"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOM76346.1"
                     /translation="MDKELLIKRIKETAYLEGDFILRSGQRSRYYLDKYLFETQPDIL
                     YQLGKEFCRHLTPDVNLIAGAELGGVALAAAAAMESVRPWIIVRNSKKGYGTGKMIEG
                     LLKPGDVVLLVEDIATTGGQVLEAAKIITDAGAKVKKIVCVIDRKQGARENIEQAGYV
                     FESIITKDDLGITD"
     gene            complement(8399..9388)
                     /locus_tag="PLE88_07505"
     CDS             complement(8399..9388)
                     /locus_tag="PLE88_07505"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOM76347.1"
                     /translation="MMPIRIPKPIFKSLYAFFTGASCCLSALLSGCADPCRCPSAKES
                     IVADGAVLKEVSAAFQFTEGPAADAAGNIYFTDQPSNRIMVYTADGRLETFLQPAGRS
                     NGLYFDRQGFLIACADENNELWRINIKTKQHSVLAQSFHGKRLNGPNDVWAAPNGDIY
                     FTDPFYKRSWWSHSDTPQDVQAVYRLAADGQTLMRVADDLEKPNGLIGTPDGKTLYIA
                     DIGAGRTYVYKIQPDGMLTDKRLFCQMGSDGMTLDCCGNVYLTGRGVTVFNPAGEKIR
                     QIDIPQRWTANVTFGGKDGKILFITAGTAVYTLQMNVCGAKQPNRPNFLTSNR"
     gene            complement(9385..10818)
                     /gene="purB"
                     /locus_tag="PLE88_07510"
     CDS             complement(9385..10818)
                     /gene="purB"
                     /locus_tag="PLE88_07510"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOM76348.1"
                     /translation="MAKNTAIYSSPLVERNASKEMAELFGADKKFSTWRRLWLELAKA
                     EKKLGLNITDAQIKQMQKHLDDIDYKKAADYEKKFRHDVMAHVHTFADAAPKAAPIIH
                     LGATSCDIGDNADLIIMREGLRILAGKLAALIDRLGRFAKQYRTLPTLGFTHYQPAQL
                     TTVGKRATLWCADFAMDLAELEYRIEQLPFRGIKGTTGTQASFLALFSGSHAKVKKLD
                     ETVARAFGFTNCCAVTGQTYPRKIDKQIVDALASIAQSAHKMCNDIRLLANLKEIEEP
                     FEKSQIGSSAMAYKRNPMRCERATGLARLVLSLAASPAMTAAEQWLERTLDDSSNRRV
                     AIAEAFLAADGILEIMLNVASGLVVYPKVIASHVAAELPFMATENILMAGVKAGGNRQ
                     ELHERIRIHSHAAAAQVKQYGLPNDLIERLQNDPAFAKVDFKKVMNPNDYIGRAPQQV
                     DEFLAGVVAPIRRKYRNALNKKTELKV"
     gene            complement(10901..11848)
                     /locus_tag="PLE88_07515"
     CDS             complement(10901..11848)
                     /locus_tag="PLE88_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOM76349.1"
                     /translation="MRKRRLGQTDLQLTVIGLGTWAIGGPWQYGWGPQDDADSLKTIQ
                     AAVDAGINWIDTAPIYGCGHAEEVVGQALKAIRPKPLIATKCGLLWNEKREKISCLDR
                     KSILKECDDSLKRLGVEAIDLYQMHWPQPDAQLEEGWEAMARLVEQGKVRWLGASNFT
                     VDQLGRVAAIHPVAAVQPPYSMLRRDIEKDLLPYCTENKIGIVAYSPMQKGLLTGKFT
                     KEYLKTLPPDDHRLHDPNFVGQRFENNLRIIEALQSIAAREGRTVGQLAVAWVLRKEE
                     VTAAIVGARKPEQIKETVSAGNWVLDDDTLDEIENVLKM"
     gene            complement(11924..12622)
                     /locus_tag="PLE88_07520"
     CDS             complement(11924..12622)
                     /locus_tag="PLE88_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOM76350.1"
                     /translation="MEPLQLCIAWYIVFVFSIVLHEFAHALIGYRFGDQTALRQGLVT
                     LNPIPHIQRSPIGMLVVPLLSFIGSGWMIGWASTPYDPYWAQAYPKQAALKTLAGPAA
                     NLLLAVAAGLLIHLGIWAGLFYPPESVRFTQVVQANADGLPAAAAVMVSILFTLNVLL
                     CVFNLIPITPLDGTALMEFILKGRPLYAYRALMAHPGLRAFGIFIAWMIMDIIYGPVH
                     TGALRALYFWHGIR"
     gene            complement(12769..13551)
                     /locus_tag="PLE88_07525"
     CDS             complement(12769..13551)
                     /locus_tag="PLE88_07525"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM76351.1"
                     /translation="MALNKQNTIWDFSGKTAIVTGGGQGIGLCIAQMFLQSGANAVIA
                     DSNADLKSKAHVFLNAPENLLFSVTDVADENAVITMTENALQRFGRIDFLVNNAAAFC
                     DAPLDTLTCRQWQTVLHVNLTGAFLCSKYCRPQLEKNRGAIVNIASTRALMSQPDTLI
                     AYSASKGGLVALTHALANTLGPAVRVNCISPGWIVTDAYQHGGRRTKLTQADSSQHPA
                     GRVGRPEDIAEMVLYLCSDKAGFITGQNIVIDGGMTIKMIYV"
     gene            complement(13541..14335)
                     /locus_tag="PLE88_07530"
     CDS             complement(13541..14335)
                     /locus_tag="PLE88_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOM76352.1"
                     /translation="MEKTQLEEYKRWFTEFVAGYYADGDGFLNQNINLKECHTHRVCK
                     EMRELAAALEMSCSDSFLAEAIALFHDLGRFPQFKRYRTYKDTVSENHCLLSLNVLSA
                     HCLLNNLPAEERAIIKKAVEFHGALKLPLLDDRTLHFARMIRDTDKLDIYNLLVQNYR
                     ILTETPDQFKWELEFPDSPECNSQIVEALLNNCLIDYRQIRTLTDAKLLQLGWVFDVY
                     FDYTLQQIRDRGYLKALIRFLPNTNSTNTVIKHIETYVETRLAGGS"
     gene            14591..14872
                     /locus_tag="PLE88_07535"
     CDS             14591..14872
                     /locus_tag="PLE88_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM76353.1"
                     /translation="MPTVSDIVVRKPSTQEIQSCQKWPIWTCGVSTFEWEYTQTEKCL
                     ILEGQVTVSNLPDNGQTISFGPGDYVQFPNGLKCIWNIKKAVKKHYDFE"
     gene            15054..16430
                     /locus_tag="PLE88_07540"
     CDS             15054..16430
                     /locus_tag="PLE88_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOM76354.1"
                     /translation="MILTVSQDYDYVTPWKKSPMSRSVGSGFIIEGNRILTNAHNVSN
                     QRYIEVKKQNLARRYPAQVLFAGHDCDLAILTVDDSNFFEGTVPLQFGGLPTINSTVQ
                     TYGFPIGGQQISITEGVVSRLETSIYSHSQAAQHLVVQTDAAINPGNSGGPVLQEGKV
                     VGVAFQGLQSADNIGYMIPTTVIAHFLKDIEDGTYHGFGMLGISLFPGLHNPGYKRYL
                     RIPDEQDGVVVTNVLVNSTSWGILETGDVIIKIDDFNVDNDGRIMIDGLSLELTEIMD
                     RKQIGQTLLVNGYRKGQPFERELVIAANKSVLPWNRLYDTKPDYRIFAGLTFVQLNRN
                     FLETWGRNWVTEMPFELRYLFMESNTLNKSPDRKEYIVLSEILPDEVNAYLAGFKNQV
                     VESVNGLNINELADLDKAFNQTSDGFWIVKFLNNNCPMVINSQQAKQREKLIFEKYQI
                     PTDKEAAL"
     gene            16427..17905
                     /locus_tag="PLE88_07545"
     CDS             16427..17905
                     /locus_tag="PLE88_07545"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76355.1"
                     /translation="MKYNSFFNIGISILLLSPLLLYAENSADLTEPMKQSLVFLEISS
                     SRYEQYQPWKQTAISKEEGFGCAVGPNEVLTTAENVINAKFVQARCYGQNEYIPAKVQ
                     VVDYEYNLCLLELDASAMERPLVPLTFAEQFPKGSQLNVYWLSSGGHLTNARCTLDRA
                     EMQNSSISFVSNLIFFAANVSRPFGDGEVCSYGNEVIGMACWGTSSDSGIIPAETINR
                     FLTHCKKEGYSGFGVPGFDIYELLDPAMRKFLKLPDEIKHGVYVGAVYTLGTGSRELK
                     SGDVILAIDGQSLNPYGRYAHPLYDRISFSNLILQKPDGSIIPFEIFRDGQKIIIHVT
                     ARNIKSKEMLIPYYLYSEQPEYIVLGGYVLQKLSRDYLTMWGSDWAGKVPPHLYHYYK
                     DLSFKPSDERKDIVVLSYVLPSEINLGYQQLSRLVVDSVNGKKIKEIKDILDIIQTDN
                     SDFIEISFELDSPTVIIQKDKLNEANMQIAQMYGIPKLINVN"
BASE COUNT         4453 a         4371 c         4711 g         4650 t
ORIGIN      
        1 attgtgctgg atgtttttgc caagctccgg caggacagcc ggctgactgc ccttcggctg
       61 gcgattatcc cgcgaaagcc cgagcggttt gacgaagttg ctgccttgat tgagcaatcc
      121 ggctttgatt atgtccgcta tagtgttctg aaggagcagg attcgcaggc tgccggcaag
      181 ccggctgttg ttttgggcga tacgatgggg gacctgaaga agttttatgc gctggcggcg
      241 gttgtttttg tcggtcggtc tttggtgccg atgggcggct cggatatgat ggagccggcc
      301 gctcttggca aatgcacaat attcggacct catactttta attttaagca gactgtagaa
      361 gtactgctgg aaggcggcgg cgctgttgaa gcagctgatg aaaaggccct ttatgaaaag
      421 accctgcagt gcctgaccga tgcggcgtat gcatctgcga ttgctgcgga gggtcaggcc
      481 atcattcgtc aaaaccaagg cgccaccaaa aagacattgg aaacaattaa tcggttatta
      541 aaacaataac agataaaatt ttttgacata tatgcttgga aattcggatt gctattccgg
      601 attatcaagc ttgttgggca ttcgatgcgt tgtcgggcgg gagaatatga cttcacaatg
      661 ggcggcgggc aatgactttg cggagttgtt ggagcggctg gaaaaatcgg catttcgcag
      721 ccgctttaag ctgggttcca aagaaagggc ttacctgaag gagaagggat ttactgttat
      781 ccagcagcat gcgcgagatt ttattgcaag tcggctggcg ccggcggaga ttgctaacga
      841 cgggaaacag actccgatgc gggggcatcc ggtttttatt gctcaacacg ccacagcaac
      901 ctgctgcaga aagtgtcttg aaaaatggca tagactcccg cagggaagag aactaacatc
      961 cgaggaggta gaccgcattg tttcaattct gctgatatgg ctcaaaagac aggcacaggc
     1021 accggcccga acagaattat tttgaataaa caggttttga aaaagtgttt ttttataggc
     1081 cggctgtttc ccattggctt ttcatataag ccagcgcctc gggaagctgg cggtcgaaat
     1141 catctgcacg gccttctatg gagatgcagc cgcggtagcg gatgtctttg agcgccttaa
     1201 aatagccggt aaagtcgaat ccggaagccc ccggccattt gcggccttct ttttcggcga
     1261 tatggcagtg cgccagcaag cggccggcag cgcggataga gtcggggcct tcgtcttcca
     1321 gcgccatatg atagaaatcg gccagtacgc gaatattcgg gtggttgact tctctggcga
     1381 tagcagtccc ttcggcgacg gtattgataa agttgcattc tttcttgttg agcggttcga
     1441 tagcgatgat gacgccgtat ttttcagcaa tcggccccat ttttttcagc agatccacga
     1501 actgccgctg tgcttgagcc ggctcgaagc cgtcgggcac cctgcgcgag cttccgctgc
     1561 cgaaaacaat gactctaata ccggccgcct gcgcccgccg gaaagccgtt tccgcatagg
     1621 ttaaaattgc cggatggtcg gcagccggtc cgacgcagat caaactggaa ggcaggaagc
     1681 tgttgcaggc atacagcggg gccttgacgg attgagcctc taaaaggttc ttggcaaaat
     1741 cctcatcgct gctttgcggc acaagaaacc gctgcacccc cagctcgata taatcatagc
     1801 cggcattctg aagcttggcg gtgttatcca atcctgtaca aactccccac tggggaatga
     1861 aagccgcatc cgatgccttt tcctgctggg gctgacagcc ctgagatgct gcaaaaaaca
     1921 ccaatattgc cgttagtgct gcgtgccgga aaatgctttt gactgccctc ataatccgtt
     1981 ccctgaatta gctgctgttt gcaattctaa agctgataaa aggattatcc ggccttggct
     2041 ctttaatttc aagcttaaat tgttttatcc gacagaatcc gaccatgcag actgacagta
     2101 atatcgcgga tgggcaaagc aatgccggcc agcgcgatcg ccttctgcgc cagcagtcgc
     2161 agtcccgaac agcagggaac ctccatatgt accacggtta agcttttagg tttagcggcc
     2221 gtgaaaatat cggccagttt ctggatatag cggccggtat catccaattt ggggcaggca
     2281 atcgctatgg cttttccgcc gagcagactg ctgtgaaaat cgcccatggc aaaggggaca
     2341 cagtcggcag tgagcatcaa gtcggcattg acaaaatacg gcgcggtcgg agacaccaga
     2401 gcaagctgga ccggccactg ccgaagctgc gacgagacat cgcctgtcac ggcggctgct
     2461 tcctgtgcaa acgacaagac ccgcatcccc ggacagacag agcctgccgc ttgggtctgc
     2521 ttctggaagg cagttttgtt ggccgccaac tgggccagat gctttttaac cgcttcttcg
     2581 tcgaagtcct cggcttgccg ctgctcgatg gtaatggccc ctgtggggca gcaatccaga
     2641 caggccccca gcccgtcgca gtaaatctcg ctgactaatc gggctttgcc gtcaatcatc
     2701 tggattgcac cctcagcgca ggctcggacg cattggccgc agccgttgca tttgttttca
     2761 tctatgttta ctatattgcg tacagccatt gtaatcctca ttgtattgaa ccgcatagtt
     2821 tgtcccagcc acttccccaa caccggcaca gctgtggggg aagcgttgtt aaccgcccaa
     2881 tatgcatcgt cctgccggcg gctgtcccct ctgcggtggt ctttaagttt ttatgccagt
     2941 atttgcgcca agtcttcctg cggcgttgca gttggggcga tgttgaattt ttccaccagc
     3001 accttgagta cattgggtcc gataaaggcc ggcagcgatg gtcccagctt gatgttcttg
     3061 attccgagat gcagcagcgt cagcaggatg cagacagcct tctgctcgta ccagctcaat
     3121 atcatcgaca gcggcaggtc attgactccg cagtcaaaag cccctgccag cgcaacagca
     3181 atctgaattg ccgaataggc atcgttgcat tggcctatgt cgagcagccg cggaatgccg
     3241 ccgatagcgc cgaacggtag cttgttaaag cgatacttgc cgcaagccag tgtgaggata
     3301 acacagtcat cggggaccag ctgggcaaac tcggtgtagt aattgcggcc cggcttggcg
     3361 ccgtcgcagc cgccgatgag gaagaaatgc cgaatcttgc cggccttgac cgcttcaata
     3421 acagctggcg ccacattcat aaccgcattg cggccgaaac cgatcgtgat ggttttggtt
     3481 tcggcatctt cggcaaatcc cggggcggca agcgctgcct gaatcaccgg tgtaaagtcc
     3541 ttatctttgc cgatgtgacg aacttcaggc caagcgacga gtcctgttgt aaagatgcga
     3601 tccttgtagc tgtcgcgggg cttctggatg cagttggtgg tcatcagaat tgcacccggg
     3661 aaggcctcaa attctttctg ctgatcctgc cacgctccgc cgtagttgcc gacgaggtgc
     3721 ttgtattttt tcaggcccgg ataggccaga cacggcagca tttcgccgtg cgtatagaca
     3781 ttgatgccct tgttttcggt ctgctggagc aggagttcga gatctttgag gtcgtgaccc
     3841 gataccacaa ttgccttgcc cttgaccggt gtgacccgta cggttgtcgg ctcgggatga
     3901 ccatatgtgc ctgtgttgcc ttgatccagc attcccatta cagtcaggtt gatttgaccg
     3961 actttcatag ccatagcgac cagcgcatcg acattggcgg ctgaaggacc ggtcagaaag
     4021 tccagcgcct catggaaaaa ggcatacact ttgtcatctt caacgcccaa gatcattgcg
     4081 tggtcagcat aggcggccat ccctttgagc ccatagagaa tcagctcttg cagaccggtt
     4141 acatccggcc cttgtgcatc gagccgtttg gcgatgctga cagttgccgc ctgctgaagc
     4201 atctgggtgc ggtttgccgg cacttgccat aaggcaggtc ccgacagcgg ttggggctgg
     4261 atgttctttt tgcgacaggc atccagatac agcgctttgg ccgccgccag cgtttgtcca
     4321 gctttggcaa tcagtgcctc cagccgaacc ggatcaaaat tgacattggt tacggttgaa
     4381 aacagggctt caacgacaaa ccggtccaca gaccgatctg cataacccaa ttgtccggcc
     4441 cgatacgcat acatggctat gcctttgaca gcctcaatca gcaggtcctg caattccgct
     4501 gtttcggcag ttttgccgca aacgccgata ttttttgcac accctttacc cccaattgtc
     4561 tgttcacact gataacaaaa cataaacggt tcctttcctg aaagagtttc ttattgttta
     4621 cccatatttt tcgatagata cagccggcag gcaatgcgcc gtccggcatg agttcgcagc
     4681 tggctgtcaa taattttctg cttttgggcc tgtgtccacg atgcataatc atcgccgaga
     4741 ttcacaatcc agtcggcatc ccagacgcag cggaattcgg gcgtatcaat tttgccgccg
     4801 ctgtgatggc tgccgacaat agcacagata tggttgatat ctgccggggg aaaggccagc
     4861 gattcgagga ttgctttggc aataggcggg ccttccagct cctgatagat accggcggca
     4921 gagtgatatt tttgctcggc ggaaagaatg ccaatgtcat gcagaacagc ggcagcgaca
     4981 acgatattcg gctgggcctg ttctttggcc ataatcgatt cggcatagtt caaaacggcc
     5041 aaggcatggt caatccgttt ctggtcgctg ccgaagaccg ctttcatttt ttcaatcagt
     5101 atctgccgca ttggattctc tgttaacagc attggattgc tatttcgcat agtcagcttt
     5161 ttttgtctct ttttcggaca ggaattatgc agaatttctg ctccgaatca ttgtcagcag
     5221 cattttgaat ctttcatcgg catggacagc gtgggggata tcagccggca taatgataat
     5281 ctgtcctgcc tgcacggtgt ggtctttgcc gttaatggtt ataactcctt ttccatcaat
     5341 aacttgaaca acggcatcaa aaggcgccgt atgttcgctg agccgttggc cggcgtcaaa
     5401 ggcaaacagt gttattgtgc cggccggctt gtccagcaga gttttgctga ctatggagtc
     5461 gttggcatag tcaataaggg cttgcagtcc tgccggtctg ccgaaacagg tctctaaaca
     5521 gttcagattg ggttctattt tattcatttc tgttctccct gtgaacaaat tcttttaatg
     5581 tcaactcatt tagaaagacg tcaatctgcg actgcagaac agctaacttc ggatgtgccg
     5641 ggcaattctt gttgagcggg caacgaaaag atccgagcag acaccgatta acgcaaacac
     5701 ggccctgaat cgcctctaca acctgcccaa accgaatctg ctggggcggc ccggctaggc
     5761 gaaaaccgcc tttggctccc atgatgcttt ctgcaacttt agcttttaca agcttttgca
     5821 gaattttgca ggcaatagca tacggcacgc tgcactggct tgacagctga cgggctgaca
     5881 gagaagtttg attttcgaat gcccctgcca gagcagccgc tatccgcaat gcataatcag
     5941 tatcaagacg aataatatcc atatactcaa aaaataatta aattcttttg tgtttcagtt
     6001 ctatctcgga ctattttagt cttacttacg gttattgtca ataatttgtt taaaaaattt
     6061 ttgaattttt ttgttacggc atcgaagaaa tcaaatcaga ctgtttagga tggcaaaaat
     6121 cagtccaaat tataggatta tagtttgggt tctggataaa atataaatag attttacaaa
     6181 acttttacga acaagaaaaa agtgctttgc gtaattttca aaatataaaa agtgtttcgg
     6241 attggagata ttttcccatt gacgcggcgt tttatcaatg cgaaaaggaa agtcaggcaa
     6301 aagtttacag gcatcttgag aatttgttta aatgattaaa gaaaggagac ttatgaaacg
     6361 gcaaatgata ttgcaggcag cagttgtact ggtcatatcg tttatgggca tgccggcatt
     6421 ggcggccgag gcggaaacaa acggcataga aacccttcgc aagaccagca aggccttttc
     6481 cgaggtcgcc aaaaaggcaa cgccggcagt ggtggcggtg aaagtacgga ccaagataga
     6541 agtaccgtcg atgggctttg gatttgggtc gccgtttgat gatgagtttt ttgaaagatt
     6601 tttcggccgc aggtttccgc agacaccgca ggagccgcag gagcggtggc gtgaggggca
     6661 ggcctccggc tttattatca gtcccgacgg ctatattctg acgaacaatc atgtaattga
     6721 tgaggccgac gaaatttcgg tggttttgca ggatggacgg actttcgaga aggttaaact
     6781 gattggggca gatgataaga cggatgtagc ggtactgaaa atcgaaaata tcgacaatct
     6841 gccgtgtgtg gagcttgggg attcagatga ccttgaaatc ggtgaatggg ttattgcaat
     6901 cggcaatccg ttcggcctga ccgagacttt gacggtcggg gttgtcagcg cgctgggccg
     6961 caagcttggc ggcgagggct ttcaggattt tattcagacc gatgcggcga ttaatcccgg
     7021 caattcgggc ggtccgctgc tgaacctcga cggcaaggtt gttggcatca attcggcgat
     7081 tattaccggc gaccgcggat atatgggtat cgggctggcg gttcccatca atatggccaa
     7141 atctatcaag gaccagcttg ttaatacagg caaggttcag cgcggctatt taggcatatc
     7201 gatgcaggaa atctcgccgg atatggcaga gttctttaaa ctcaaagaca gcaagggagc
     7261 cctgattacc gatgttgtca aggattcacc ggcagagcgg gctggtctgg agaaggacga
     7321 tgtgattatt caaatcggca gcaaaaagat tgaagatgtg caggacgttc gcaatgttat
     7381 cggttttact cctcccgata cgattgttga gatgacgatt atacgcaacg gcaaggagaa
     7441 gaagctcaat gtcaaggttg gctcgcagga agaaagcgaa ctggccgata cttccgagac
     7501 cggcaagcag ctgggactgg aggtagcagc cattgatgaa gaaaaggcca gacagtataa
     7561 ggcggctgtt ggcaagggag tggtaataac ccaagtcaag agcggctcgg ctgcggcgtt
     7621 tgccggcctg cgcgaaggga tgattattta cgaggtcaat cgggtttccg taaacactgt
     7681 aagcgaattt aatcaggccc ttaaagaatc tgagaaaacc aaaaaagtcc tgctgctggt
     7741 taaagtggga cgctatacgc agtatgttgt gttgtcgctg aaaccgtagc agcggcatgg
     7801 aaaaaaaagt aagcccggat gcctgttgca tgtccgggct tttttattgc gcctcagtca
     7861 gtctgtaatg cccaaatcgt ctttggtgat aatgctttca aagacataac cggcctgttc
     7921 gatgttttcg cgtgctccct gcttgcggtc gatgacacag acgattttct tgacctttgc
     7981 gccggcatcg gtgatgattt tggccgcttc gagtacctgc ccgccggtgg tggcaatatc
     8041 ttccaccagc agcactacat ccccgggctt aagcaggccc tcaatcattt tgcctgtgcc
     8101 atagcccttt ttgctgttgc ggacgataat ccacggtctg acagattcca tagccgccgc
     8161 cgcggccagc gccacgccgc ccaattcggc gccggcgata aggtttacat ccggcgtcag
     8221 atggcggcag aattctttgc ccagctgata taaaatgtcc ggctgggtct caaacaggta
     8281 tttatccaga taataccggc ttcgctggcc gctgcggagt ataaaatccc cctccagata
     8341 ggccgtttct ttaatgcgtt tgatcagcag ttctttgtcc acgtttgtct cctgattttt
     8401 atcgatttga agttaaaaaa ttcggtctgt tcggctgctt ggcaccgcag acattcattt
     8461 gcagagtata gacagcagtt ccagcggtaa tgaaaaggat tttgccgtct ttgcctccga
     8521 aggttacatt ggctgtccag cgctggggga tatcaatctg gcgtattttt tccccggccg
     8581 gattgaaaac cgttacaccg cggccggtca gatagacatt gccgcagcag tccagtgtca
     8641 ttccatcgga gcccatttgg cagaacagcc gtttgtcggt cagcatgccg tcgggctgga
     8701 ttttatagac ataagttctg ccggcaccga tatcggcaat ataaagagtt ttgccgtccg
     8761 gtgttccaat cagaccgttg ggtttttcga ggtcatcggc aacccgcatc agtgtttgcc
     8821 cgtcggcggc gagccgataa acggcctgta catcctgcgg cgtatcgctg tggctccacc
     8881 atgagcgttt gtagaacgga tctgtgaaat aaatgtcgcc gttgggagcc gcccagacat
     8941 cattgggtcc attcagacgt ttgccatgga aggattgcgc taaaacagaa tgctgttttg
     9001 ttttgatgtt tattcgccaa agctcgttgt tctcatcggc acaggcaatc agaaatccct
     9061 gtcggtcaaa ataaagaccg ttggaccgcc ctgccggctg cagaaaggtt tccagccggc
     9121 cgtcggcggt atagaccata atccgattgc tgggctggtc agtaaaatag atattgccgg
     9181 ccgcatctgc cgccgggcct tccgtgaatt ggaatgccgc ggaaacttcc ttcaaaaccg
     9241 ccccgtcggc gacgatagat tctttggctg atggacagcg gcagggatcg gcgcagccgg
     9301 aaaggagcgc agacaaacag caggaagcgc cggtaaaaaa tgcatacagc gatttgaaaa
     9361 ttggctttgg aatccggatt ggcatcatac tttcagctcc gtttttttgt tcaatgcgtt
     9421 tcggtatttt cggcggatag gagcgacaac accagcgagg aattcatcaa cctgctgggg
     9481 agccctgccg atgtaatcgt tgggattcat cacttttttg aaatccacct tggcaaaagc
     9541 cggatcgttc tggagccgct ctatcaggtc attgggcagg ccgtactgct taacctgtgc
     9601 cgccgccgcg tgcgaatgaa tgcggatgcg ctcgtggagt tcttggcggt tgccgccggc
     9661 ctttacgccg gccatcagga tattttccgt tgccataaac ggcagttcag cggccacatg
     9721 ggaggcaatg accttcggat agacgaccag ccccgaggcg acattgagca taatctccaa
     9781 aatgccgtcg gctgccagaa aagcctcggc aatagccacg cggcggttgg aggaatcgtc
     9841 taaggtgcgc tccagccatt gctcggcggc ggtcatcgcg ggactggcgg ccagcgacag
     9901 gaccaggcgt gccaatcccg tggctcgttc acagcgcatc ggattgcgct tgtaggccat
     9961 tgccgatgag ccgatttgcg atttttcaaa cggttcttcg atttctttca agtttgccag
    10021 aagacggatg tcgttgcaca tcttgtgcgc tgactgtgcg atagaggcca gtgcatcgac
    10081 aatctgtttg tcgatctttc gcgggtaggt ctggccggtt acggcgcagc aattggtgaa
    10141 accgaatgcc cgtgcgaccg tctcatccag ctttttgact ttggcgtgac tgccgctgaa
    10201 cagtgccaga aacgaggcct gcgtgccggt ggtgcctttg atgccgcgga acggaagctg
    10261 ctcgatgcgg tattccagtt cagccaaatc catcgcaaag tcggcgcacc acaaggtcgc
    10321 ccgcttgccg acagtcgtca attgggcggg ctggtagtgg gtaaagccca gcgtcggcag
    10381 tgtgcgatac tgtttggcaa agcggcccag ccggtcaatc agggcggcca atttgcccgc
    10441 cagtatccgc aggccctcgc gcataatgat gaggtcggcg ttgtcgccga tatcgcagct
    10501 ggtagcgccg aggtgaataa tcggggcggc cttcggcgct gcatcagcaa aggtgtggac
    10561 gtgagccatt acatcgtggc ggaatttctt ttcgtagtcg gcggcttttt tgtagtcaat
    10621 atcatccaga tgcttttgca tctgtttgat ttgtgcatcg gtgatattaa gccccagttt
    10681 cttttcggcc ttggccagtt ccagccataa cctgcgccat gtcgaaaatt tcttatccgc
    10741 cccgaacagt tcagccattt cctttgaagc attccgctcc accagcgggc tgctgtaaat
    10801 cgccgtattc tttgccatag cttaaatcct ttttgaaatt aggcatctca aagaaaacaa
    10861 gaaagatgca tattttttct ggagctgtta ttccgctttc tcacattttt aatacattct
    10921 ctatttcatc aagggtatcg tcatccagca cccaattgcc ggcagagacg gtttctttaa
    10981 tttgttccgg ttttcgggcc ccgacaatag cggcggtaac ttcttctttt cgcaagaccc
    11041 acgccacggc aagctggccg acagttctgc cctcgcgggc agcaatggat tgaagggctt
    11101 caataatccg cagattattc tcgaatcgct gcccgacaaa attggggtcg tgcaggcggt
    11161 ggtcatcggg agggagggtt ttaaggtatt ctttggtgaa tttgccggtc aacaacccct
    11221 tctgcatcgg gctgtaggcg acaatgccga ttttgttttc ggtacagtac ggcagcagat
    11281 ccttttcgat atcccgccgg agcatactgt acggcggctg cacggcagcg acggggtgaa
    11341 tagcagcaac ccgccccagc tggtcaacgg taaaattgga agcccccaac cagcgcacct
    11401 tgccctgctc taccagccgg gccattgctt cccagccctc ttcgagctgt gcatccggct
    11461 gtggccagtg catctggtag aggtcgatcg cttccacgcc cagccgcttt aagctgtcat
    11521 cgcattcttt gagaatgctt tttcggtcaa gacagctgat tttttcgcgt ttttcattcc
    11581 ataaaagacc gcatttggtg gcaatcaggg gtttcggccg gattgccttc agtgcctgcc
    11641 ctaccacttc ttcggcatgg ccgcagccgt aaatgggagc agtatcgatc cagttgattc
    11701 cggcatccac agcggcctgt attgttttca gggaatcggc atcgtcctgc ggtccccagc
    11761 cgtactgcca cggtccgccg atagcccacg tgccaaggcc gataactgtc aattgcaaat
    11821 ctgtttgtcc gagtcttcgt ttccgcatac aggcgactcc tatttttctc ttgcaggttt
    11881 gcagtgtacc accgaagtac cctttcggca agcggtcagg ttcctaacga atgccgtgcc
    11941 agaagtagag agcgcgcagc gcccctgtgt gaacgggtcc atagataata tccataatca
    12001 tccacgcgat gaagataccg aaagcccgca atcccggatg agccatcagg gcacgatagg
    12061 cataaagcgg ccggcctttc aggataaact ccatcaaagc cgtgccgtcc aaaggcgtaa
    12121 taggaatcag attgaacacg cacagcagca cattgagcgt aaacaggata ctgaccatca
    12181 cagcagccgc agccggcaaa ccgtcagcgt ttgcttggac tacttgggtg aagcgaacgg
    12241 attcgggcgg ataaaacaat ccggcccaga tgcccagatg aatcagcaga cccgccgcaa
    12301 cagccagcag gagattggcc gcagggccgg ccagagtttt aagagctgcc tgcttgggat
    12361 aggcctgcgc ccagtatggg tcatatggcg tgctggccca gccgatcatc cagccgctgc
    12421 cgataaaact cagcagaggg accaccagca tccctatcgg agaccgctga atatgcggaa
    12481 tcggattgag cgtaacaaga ccctgccgca gggctgtctg gtcgccgaat cgatagccga
    12541 taagcgcatg ggcaaattcg tgaagtacta tcgaaaagac aaatacaatg taccaagcaa
    12601 tgcaaagctg caaaggttcc atattcgttt ccagagtcgt tcggtttttt attcagctca
    12661 tttgcatgaa cagatacttc actataatgt catacatatc tgccggcaag attccgtact
    12721 gtcttgtgga aaaagggtct gtgtttttcc gcaagagcgg cagaactgtc aaacataaat
    12781 cattttaatg gtcataccgc cgtcgatgac gatgttttgg cctgtgataa agccggcttt
    12841 gtcagagcag aggtaaagaa ccatttcagc aatatcttcg ggacggccga cccgccctgc
    12901 cggatgctga gagctgtctg cttgagtcag tttagtccgg cgtccgccat gctggtaggc
    12961 gtcggttaca atccagccgg gactgatgca gttgacccgg acggctggcc ccagcgtgtt
    13021 ggcaagcgca tgagtcagcg ccacaagccc ccctttgctg gccgaataag ctatcagcgt
    13081 atcaggttgg ctcatcaagg cccgtgtgga agcgatattg actatagcac cgcggttttt
    13141 ttcgagctgc ggtctgcagt atttggagca taaaaaagcc cccgtcaggt tgacatgcaa
    13201 gaccgtctgc cattgccggc aagtcagagt atctaacgga gcatcgcaga aagccgcggc
    13261 gttgttgacc agaaaatcaa tccgcccgaa gcgctggagg gcattttctg tcatcgtgat
    13321 gacagcattt tcgtctgcaa catctgtaac cgaaaacagc agattctccg gggcgttgag
    13381 aaatacgtgg gccttggatt tcagatcggc attgctgtcg gcaataaccg cattggcacc
    13441 ggattgcaga aacatctggg cgatgcacaa gccgataccc tgcccgccgc cagttacaat
    13501 ggccgttttt cctgaaaagt cccagattgt gttttgtttg ttaagagcca ccggccagcc
    13561 gtgtttctac atacgtttca atatgtttta taaccgtatt tgtcgaattc gtatttggaa
    13621 ggaaacggat cagcgccttt aagtagccgc ggtcgcgtat ctgctggagc gtataatcaa
    13681 agtacacatc aaacacccat cccagctgca gcagcttggc atcagtgagc gttcgaatct
    13741 gacggtagtc aatcagacaa ttgttcagca gggcctcaac gatctggctg ttacactcgg
    13801 ggctgtcggg gaattcaagc tcccatttaa attggtccgg cgtttcagtt aaaatgcggt
    13861 aattttggac cagcagattg tagatatcca gtttgtcggt gtcccgtatc attcgtgcaa
    13921 aatgcagcgt gcggtcatcc aaaagaggca gcttcagcgc tccatggaac tccacggcct
    13981 tcttgatgat tgcccgttcc tcggcaggca gattattcag cagacagtgg gcactcaata
    14041 cattcagcga cagaagacaa tggttttcgc tgaccgtatc cttgtaggtt cgatatcgct
    14101 taaactgggg aaaccgccca aggtcatgaa acagggctat ggcttctgcc agaaaagaat
    14161 cagagcaaga catttccaaa gcagcggcca gctcccgcat ttctttgcag acccgatgtg
    14221 tgtggcattc cttcaagttg atattctgat tgagaaagcc atcaccgtcg gcataatagc
    14281 cggcgacaaa ttctgtaaac catcgtttgt attcttcaag ctgcgttttt tccatagctg
    14341 tatccttatt gtctattcaa acaaactcct gtcccccgtc ttcttgcgac cggagtgccg
    14401 gagcttgact atgccataag cgttttattg gtttagaccc atcaatgtgt tgtatcatac
    14461 caaacagccg gcaggaatcc agcaggcact tgatatgatg gaaatttttg aggaaaattt
    14521 cgggcaaagc ttgccggcag gcagtatagt ggaatataga aaattttttt ttgacagaaa
    14581 ggatatgctt atgccgactg ttagcgatat tgttgtgcga aaaccgtcaa cacaggaaat
    14641 tcaaagttgc cagaaatggc caatctggac ctgcggcgtc agcacatttg aatgggaata
    14701 tacccaaacc gaaaaatgcc tcattctcga aggccaagta accgtcagca atctgcccga
    14761 taacggtcag acaatttcgt tcgggccggg ggattatgtt caatttccaa atggattgaa
    14821 atgtatctgg aatatcaaga aagcagtcaa aaagcactac gattttgaat aaaccaacca
    14881 gttggttttc ataaaaaatt aaatatatgg tgttttggaa agaatataag atgtttatca
    14941 gcagtaaatt acataatcga acgtggttcc agggcaagat attaggactg ttaattattt
    15001 tagccagtct atgctctgcc caaggttcag atacctatga aaagtccatc gtgatgatct
    15061 taactgtcag tcaggactat gattatgtaa cgccttggaa aaaatctccg atgagccgct
    15121 ctgtcggttc tggttttatc atagaaggca atcggatttt aaccaatgct cataatgtct
    15181 ctaatcagcg gtatatagaa gtaaaaaaac aaaatctcgc ccgacgttac ccggcacaag
    15241 tgctgtttgc cgggcacgat tgtgatttgg caatcttaac cgtcgacgac agcaattttt
    15301 ttgaaggtac tgttcccctg caattcggtg ggctgccaac aataaactcg actgttcaaa
    15361 cctatggttt tcccataggc ggccagcaga tatccatcac cgaaggagtt gtatctcgtt
    15421 tagaaacaag catatacagc catagccagg cagcgcagca ccttgttgtt cagacggatg
    15481 cggctatcaa tcccggcaat tcaggagggc ctgttttgca ggaaggaaaa gttgtggggg
    15541 tggcttttca ggggcttcag tcggccgaca atatcggcta tatgataccc acgactgtta
    15601 ttgctcattt tctcaaagac attgaagatg ggacatatca tggctttgga atgctgggaa
    15661 tatccttgtt tcccggtctt cataatccag gttataaaag atatttaaga ataccggatg
    15721 agcaggatgg agtagtagtt acaaatgtgc ttgtcaacag tacctcgtgg gggatacttg
    15781 aaacagggga tgtaattata aaaatcgacg atttcaatgt tgataatgac ggccgaatta
    15841 tgattgacgg attgtcgctg gagctgacag agattatgga tcgcaagcaa atcggccaga
    15901 ccctgctggt taacggctat cgcaaaggcc agccgtttga gagggaactt gttattgcgg
    15961 ccaataaatc tgtattgcct tggaatcggc tgtatgatac caagccggat taccggattt
    16021 ttgcagggct gacatttgta cagctcaacc gtaattttct tgagacctgg ggacgcaact
    16081 gggtaactga aatgccattt gagctgcgct atctgtttat ggaatccaat acgcttaata
    16141 agagccctga tcgaaaggag tatattgtgc tttcagaaat cctgcccgat gaggtcaatg
    16201 cttatttggc cggatttaaa aaccaggttg tggagagtgt taacggcttg aacataaatg
    16261 agttagcaga tcttgacaag gcctttaatc agaccagtga cgggttttgg attgtaaaat
    16321 ttcttaataa taattgcccg atggtaatta attcccaaca ggccaaacag cgagaaaaac
    16381 tgatttttga aaagtatcag attcccacgg ataaagaggc agcgttatga aatataacag
    16441 ttttttcaat attggcatca gcatattgtt gctttccccc ctactgctgt atgcagaaaa
    16501 ttcagcggat ttgacagagc cgatgaagca gtcgcttgtt tttctggaga tttccagcag
    16561 ccgttatgag cagtaccagc catggaagca aacggcaatc agcaaagagg aaggtttcgg
    16621 ctgtgctgta ggaccgaatg aagtgctgac aacggcggaa aatgtaataa acgcaaaatt
    16681 tgttcaggca agatgttacg gccaaaacga gtatatacct gccaaggtgc aagttgtgga
    16741 ttatgagtac aatttgtgtc tgctggaact ggatgcctcg gcgatggaaa ggccattggt
    16801 tcctttgacc tttgccgagc aatttcccaa aggcagtcag ctgaatgtct attggctgtc
    16861 cagcggagga catttgacaa atgcccgctg tacgctggat agagctgaga tgcagaattc
    16921 gagcatctcc tttgtatcta atttgatctt ttttgctgcc aatgtgtcgc gtccatttgg
    16981 agatggagaa gtatgctcct atggaaatga agtaatcggc atggcctgct ggggaaccag
    17041 ctccgattca ggaattatcc ctgcagaaac catcaatcgc ttcttaactc attgtaaaaa
    17101 agaaggttac tcaggcttcg gcgtgccggg atttgatata tatgaacttc tggatcctgc
    17161 aatgcgcaag tttctgaaac ttccggatga gattaagcat ggtgtttatg tgggtgctgt
    17221 ttatacactt ggaaccggca gccgagagct gaaaagcggc gatgttattt tggcaattga
    17281 tggacaatcg ctgaatcctt atggccgcta tgcacatccc ttatacgacc gtatctcttt
    17341 ttcaaatcta atcttacaga aaccagacgg ctctattata ccctttgaaa tctttcgcga
    17401 cgggcagaaa ataatcattc atgtaacggc aaggaacata aaatcaaaag aaatgctgat
    17461 accatactat ctgtattcag aacagccaga atatattgta ctcggaggct atgtcctgca
    17521 gaaactttcg cgtgattatc ttacaatgtg gggaagcgat tgggcaggca aagtgccccc
    17581 ccacctttac cattattata aggatctgtc ctttaagccc tctgatgagc gaaaggatat
    17641 tgttgtgttg agttatgttt tgccgtcgga aattaatttg ggctatcagc agcttagccg
    17701 gcttgttgtg gattctgtaa acgggaaaaa aatcaaagaa ataaaagata ttctcgatat
    17761 tattcaaaca gacaattcag attttataga aatcagtttt gagctggact ccccgactgt
    17821 gataattcaa aaagataaat taaatgaagc taatatgcaa attgcccaaa tgtacggtat
    17881 tccaaaactg ataaatgtta attaaatata caaatgcgaa agagatgttt cacatgaaac
    17941 atctcttttt ttaatgctct tcctcccctt cattatcatt agtttcacgt gaaacataga
    18001 aaaaatttat tttcttgtgg caaattgtaa acgattttga tttattaggc cgacgattgt
    18061 taggcatcat atttttttaa cggaggccat tatgacagaa tctaagaaaa tcaaaaagcc
    18121 gcatttagga cgaggattag aagctcttct cgggccggta acaaagattc aggaaaatgg
    18181 gccgg
//
LOCUS       DAOTBO010000054        18157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2548_length_18157_cov_5.658436, whole genome shotgun sequence.
ACCESSION   DAOTBO010000054 DAOTBO010000000
VERSION     DAOTBO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2548_length_18157_cov_5.658436"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..2150
                     /locus_tag="PLE88_07550"
     CDS             162..2150
                     /locus_tag="PLE88_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76356.1"
                     /translation="MVDGLNGFEKWIGYFAIDENERYEGRTQRIDRIHFLDRTLYIDG
                     PTNRYTPGYHPGPAEPQLRSLFYLPDGPMPSTDWIQTSPNNNPGLWQISSGQALQSSQ
                     SCFSFNLVNRDAATCYLFEANVTMPQPRDSEDKAGVVAYYKNSTNWVIIGLDRSLGYG
                     ADNWYCHIRTDAYNGVVAAGGFGGRLNYGVYHKIRVERNSTLFRVWIDDMLPPGFSNI
                     QTSITDAGVPGLYSDHAAALFDGIIYTIGWDEYGSNITGWGNGLNGSRQIGTWTVGAD
                     GIAVTGGGTIYKGDLMEAYEFSTHLYKTGIAENRMGIYAAAIDANNFLRAYFDLPADT
                     FVVDGKQNGQHLSPQIINIPNQQDYNLRAVKLPDRVIFFVDGREVLSRSICFPASQTA
                     LFADNMSARFNGILAYRIEAETLPSSWKHTDVGSVGFKGSASYNEGTFTVNGSGSDIW
                     LIQDGFHFVYQDLYGDGEIIARVVSNDITDWWNKAAVMIRDGLNSSAGMALVTLCGGG
                     NLQFLWRTGPGVNAQGIDAPNPPYPQGVWLRLKRTGRQYDGYYSYNGSNWTLIGSRTL
                     NFSSHLQIGLAVTAHHNNRLNGAVFDNVAIGGCTPGEYQTDLNRDCTIDISDLLVLAG
                     QWLDTVSAGTSADLDGNLKITLGDYAILASDWLIDYRY"
     gene            complement(2262..5180)
                     /locus_tag="PLE88_07555"
     CDS             complement(2262..5180)
                     /locus_tag="PLE88_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM76357.1"
                     /translation="MKKNCFWQIALLGILVVVLAACGVFLSSESWGPDESTQKVESSF
                     QDRPFDSDWRFFRGDAAGAQQPDYDDTSWPVLDLPHDWSIEDLPPADGSIPELEAITG
                     LWRFRKGDDADWKARQLNDSDWQLVLLPDTWEHHSDHTADNVFGWFRRHLEIPDNCKG
                     KDFDLLLGCIDDVDEVWFNGWRIGGTGSFPPNYQTAWTIQRRYRVPALLVRGDGTDVV
                     AVRVFDGSGSGGIYQAGSKSVRIGPFDPVLSAGGHFTGYTVGGIGWYRKHFTVEQPNK
                     QVSVLFDGVYMNAEVWLNGRRLGGHPHGYTSFEFDLTRCLNAPGQDNVLAVRVRNEGK
                     NSRWYSGSGIYRHVQLLINELIHVPTGGVFITTPEVSADKALVKAAVEVYNAAAVPTQ
                     AVVRTELQDADGRLAGRVETALSLSAVQTHSAEICIDVPSPRLWSPETPNLYTAKIEV
                     VTAGKISDAVVVPFGIRTIEFDAHNGFRLNGRTVLLKGANLHHDNGPLGAAAIDRAEE
                     RRVELMKANGFNAVRCSHNPPSSAFLEACDRLGMLVIVEAFDQWNEAKENRTEGYQRF
                     FSDWHQQDIASMVRRDRNHPSVIMWSIGNEIPEQFRDEETAKRLRQAVLLHDTTRPVT
                     QAICSDWGKVARSWDELSGLAFKHLDVAGYNYLPDKYESDHVRYPQRVMYGSESFPKE
                     ALLYWSLVEEHPYVIGDFVWSGMDYLGEAGLAHAVLSSQPESFFMSWPWFNAWCGDLD
                     ICGFKKPQSFYRDVVWRRSPIEIFVHRPIPPGLAEKLSWWAWPDELQSWNWPGQEGRP
                     LQITVYSRCETVRLELNGKVVGQQPVSPTLTAQFRVPYAPGELCALGLISGKVAAKAT
                     LKTAGPPKKLRLTADRTSIHASRSDLAYVTVEVVDADGQRVPDAEVLVHFSVMGAGEL
                     AAQGSGVPNEPASFKSPQRKTFQGRCLAILRPRGGAGDIVLHAQAEGLDREKITIHTR
                     "
     gene            complement(5256..6617)
                     /gene="bioA"
                     /locus_tag="PLE88_07560"
     CDS             complement(5256..6617)
                     /gene="bioA"
                     /locus_tag="PLE88_07560"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOM76358.1"
                     /translation="MNNRTKQLIELDKRYLWHPFTQMADWLACEPVIIESGEGFYLID
                     TEGRRYIDGVSSLWCNVHGHRVRKIDDAIRSQLDKIAHSTLLGLGQTASIELAEKLIQ
                     IAPKGLTKVFYSDSGATAVEIAVKIAYQFWQNIGQKGRTKFIALEEAYHGDTVGSVSI
                     GGIELFHGIFKSLLFETYYVPSPHPYRFDGTAEQCVASSLEKMEFLLKEHAGRIAAIV
                     VEPLVQGAAGILVHPKGFLKGAETLARKYDTLLIADEVATGFGRTGRMFACEQEDVHP
                     DLMCIAKGISAGYLPLAATLTTQKIFDAFCVCGDICKTFYHGHTYTGNALGCAAALAS
                     LELFEEHRILETLPEKITLISRFLPRAAILPYVGDVRQCGMMAGIELVKDKNTKEPFP
                     YPLRLGAKVCSAMRPKGAMMRPLGDVIVLMPPVGIDVQTLDTLLHIIEDTLINELPKI
                     AAG"
     gene            6740..8782
                     /locus_tag="PLE88_07565"
     CDS             6740..8782
                     /locus_tag="PLE88_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOM76359.1"
                     /translation="MSIEKICLFFFLAASPLWAGYSLFVSPTGDDTAAGTSPENPIKT
                     LQKSIDLARQIKNQPVIVYLKGGDYEVTKPLTFGMDDSRTADNPLTFKPFENEKPIVF
                     GGRRIRNWQKQPSGIWTAQIDEVKNGQWYFSQLFINGQRRRRARIPNEGFLRVAGFPD
                     GGRDVHYHTDCQRFEYKPGDIDPNWVNMEDVEVIVYHFWTDSHLPIASIDPNTHIVTF
                     KHKAGKIFTDDFTSTGARYIVENVYEGLDAPGEWYLDRKTGVLSCIPMPGEDIAEAIA
                     PVAPELVRIEGSPSDMRYSEHIRFEGIEFAYTHWLLPPGNSNDAQGASSVPAAITLTG
                     CRSICFDRCTVRDLGTFAFDIGAGCREITISRCTLKNLGGGGIRISGGSFSSPPLLRT
                     GRNVIADNHIGPYGEVYPSAVGVLLMHTFGNTICHNEIHHGWYTGVSVGWEWGYQPSI
                     SRDNIIEYNHIHHIGQGLLSDMGAIYTLGVSPGTIIRNNLIHDIDSNHYGGWGIYNDE
                     GSSHILVENNIVYNTKFAGYNIHYAKEIIVRNNIFAYGRFQQLSRGRMEPHRSCFFEN
                     NIVYFTEGALLDGNWKDASYVFYYNPNEPKGRETLSTFEIDWNLYYNPALPLEKVSFN
                     GQTFADWQKRGKDRHSLYADPLFVNPDSFDFRLKQDSPAMKLGFVPIDTSTVGPRP"
     gene            complement(8807..9370)
                     /locus_tag="PLE88_07570"
     CDS             complement(8807..9370)
                     /locus_tag="PLE88_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76360.1"
                     /translation="MKTSYLLLSASLLGILAGCVPSLHQLWTDKTLIFDDTIAGTYKQ
                     DESTWVFVGNPQEKSYTLTISEKEGRQSKLIARLVEVSGKRFFDFYPATDAELEAGEW
                     LKLHILPVHLFFKVEKTAESFKLAAMNPDEVGKLLKEKPQLIKHEVVEDDRVVLTDRA
                     ENLQKFLLEGLAVEKFFGDPMELCPVK"
     gene            complement(9474..9875)
                     /locus_tag="PLE88_07575"
     CDS             complement(9474..9875)
                     /locus_tag="PLE88_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOM76361.1"
                     /translation="MSYNEDTIDDAKCPLNWDDVYGRIGDESIIEEFAGAFLKNSLTL
                     MLSLKSAVASISPEQIELYAHALKGSASNMGAIALAKKAWRLEKAAAEKKLDNVGELF
                     AAVKKEYLDLKEFFENPDWIARAKQMPAKVS"
     gene            10055..10699
                     /locus_tag="PLE88_07580"
     CDS             10055..10699
                     /locus_tag="PLE88_07580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOM76362.1"
                     /translation="MARRAMVDSDLRGRDINDSRVLKVMAEIPREAFVPAKYVGQSYA
                     DYPLPIGMGQTISQPYMVALMTQCLELSGTEEVLEIGTGSGYQTAILSKLAARVYTIE
                     RFCELAQSAQTVLSNLGLTNIEYYIGDGSTGWPEPRHFDRIIVTAGVPEVPPPLIDQL
                     KDGGLLTAPIGGQWSQELIVGRKQNNQLTTRTVCGCRFVPLIGRYGYSEPKEMR"
     gene            10996..12216
                     /gene="tyrS"
                     /locus_tag="PLE88_07585"
     CDS             10996..12216
                     /gene="tyrS"
                     /locus_tag="PLE88_07585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOM76363.1"
                     /translation="MTLDVTEQVKLLKRGTVEVYREDELAQRLAEAAKEGRQLRAKLG
                     MDPTAPDIHLGHTVVLRKLRQFQDLGHKAVLIIGDYTARIGDPTGQNATRPMLSPEQI
                     EQNATTYFQQAGKILDMSPDKVEVRFNSEWLADLRLADIIKLTASMTVARMLERDTFE
                     IRYKKGDPIGIHEFLYPLMQGYDSVAIKSDVELGGTDQTYNNLVGRDLQKLDGQKPQI
                     VITMPILVGLDGVQKMSKSKGNYIGVTDSPADMFGKTMSIPDNLMENYYTLLTDIPVD
                     EIKVLCDPQQTHPKEAKVKLGKLIVQQFYDAPTAQKAAEEFDKIFAQNQLPEQMPEVE
                     IPAEPIMISKLLVQCGLTATGGEARRMCIQGGVRIDDQRYPDPAAVVTPVSGMVVQVG
                     KRKFAKLKIIEQPI"
     gene            12266..12847
                     /locus_tag="PLE88_07590"
     CDS             12266..12847
                     /locus_tag="PLE88_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76364.1"
                     /translation="MMKLPQLTEPDKYTGFYAVDFGDHSGVGFTAEEVAELLESEKFS
                     DIKVYRICRAYPDGTLELKGVRRETFQMEAGMFFYAADEEQARMDFDRLLDWANRQPA
                     PCRAKVHLARLGNSFVTALIYPAEYDDDISRWLLDGHYRTCGAAEGGTGAVNRYYQAD
                     SQVLDRRQLWPASSIEHLQGQALLEAAKRAIVR"
     gene            12844..13059
                     /locus_tag="PLE88_07595"
     CDS             12844..13059
                     /locus_tag="PLE88_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76365.1"
                     /translation="MKSVCSGRHRHLQKHLGWLAGLFLQRFGRKPPEKNAAHMYKQDF
                     STSTQRLGLRFTDRLRDVFRRHWLRLK"
     gene            13174..13260
                     /locus_tag="PLE88_07600"
     tRNA            13174..13260
                     /locus_tag="PLE88_07600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13208..13210,aa:Ser,seq:cga)
     gene            complement(13538..15337)
                     /locus_tag="PLE88_07605"
     CDS             complement(13538..15337)
                     /locus_tag="PLE88_07605"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOM76366.1"
                     /translation="MVDRNLIYKLGLTNEQIEQEVGELFTPEHTQLLAEELEKKVEAL
                     VPGTIVKGKIVTQLGNDVIVELGLKSEGIVDAGEFDDPSEIVPGKEIEVLLEEMDAES
                     GILLSKRKADRIRGWERVIASNKEGDVVKGMVTRRIKGGLLVDIGVPVFLPASQVDIR
                     KPGDISRFIGQEIECKILKIDTENHNIVVSRRKLIEEDRQASKERILREIEVGQIRKG
                     IVKNIADFGVFVDLGGLDGLLHISDLSWGRISHPSELVQLDQEIECMVIGVDRENEKI
                     SLGLKQKQPSPWESAEQRYPVGKRVKGTIVNIMNYGAFVRLEEGIEGLIHISEMSWTK
                     RIAHPGDILTLGQEVEVMVLDVNKEKQEISLGLKQLETNPWTIAGQKYPVGTMVTTKV
                     TSLTNYGAFVEIEPGIDGLIHISDLSWTKKYNHPGEALQKGQEVKCVVLEVDEEKHRV
                     SLGVKQLTEDPWIRAIPEKYIPGQIVKGVVTKLTNFGVFVELEPDLEGLLHISELSDS
                     KVDSPQDVVKIGDVLEVKILRVDTDSRKIGLSLRRVQWAAEDKAAEAAASGQTTASAP
                     IEENESSPRRGGLDGTLMPDSLNIPMPGKKQNP"
     gene            complement(15481..16335)
                     /gene="ispH"
                     /locus_tag="PLE88_07610"
     CDS             complement(15481..16335)
                     /gene="ispH"
                     /locus_tag="PLE88_07610"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOM76367.1"
                     /translation="MKVLVAEKCGFCPGVRNAINLARKTLAEEGKAYSLGHIIHNEDV
                     VRQLSEAGLKPVDSIDEVQNGTVLIRSHGATQEEFEKINKKGLKIVDATCVLVRRVQK
                     IAKLLNDEGYKVVIIGDTNHPEIRAVVGYAKDIAVVGNEEDLDKLNLTKKIGVICQTT
                     QSPDHFADMIAAIARRGFTEMKIINTLCRETMERQQAAVKLCRQVDIMFVLGGLHSAN
                     TRKLAELCKKHNPQTYHLQNWAEFDKKYLFGKTTAGVTAGASTPDEVIEEFVRNLEQW
                     SPPAETDC"
     gene            16555..17115
                     /gene="efp"
                     /locus_tag="PLE88_07615"
     CDS             16555..17115
                     /gene="efp"
                     /locus_tag="PLE88_07615"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOM76368.1"
                     /translation="MKVSEMKKGQTIMMDGILYVIVDYQHVKLGKGGAVYQTKLKNLT
                     DGGIRDVRLRSEETVEEAYLEKRNFEYLYSSPNEHVFMDLESFDQITLDDDAFGDGAM
                     YLKGNEKVQISMYKGKPVVITLPNTVDLTVTDTAPEIKGATATNQYKPATLETGVVVQ
                     VPPFIKVGEKIRVDTRTGEYVTRVKD"
     gene            17328..>18157
                     /locus_tag="PLE88_07620"
     CDS             17328..>18157
                     /locus_tag="PLE88_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76369.1"
                     /translation="MALAAFNKFLVGIFGSRNERIVKSYMQMAQAAGKLESHIQTLTD
                     EQLKAKTDEFKQALRDGRRPQDILPEAFAVVREAARRHIAMRHFDVQLVGGNVLYDGK
                     IAEMATGEGKTLVATLAAYLVHLTGRKVHIVTVNDYLAKRDAEWMGPAYRALGLSVGA
                     IQAEMDTSGDERKQQYACDITYGTNNEFGFDYLRDNMKISLSQMAQGPLEYAIIDEVD
                     SILIDEARTPLIISGPAVDDVSRYKKADSVARQLISLQAAYNNLKKAIETAERTLANA
                     "
BASE COUNT         4620 a         4656 c         4336 g         4545 t
ORIGIN      
        1 ctactcgaac cgatggttta tagcgttttt ccgagaaccg gactcggaag tttgccagca
       61 gtttcagcaa tgccggcaaa cttccgagtc cggttctcgg aaaaacgcta tataccatcg
      121 gttcgagtag ttttaccatc tatacgctgg gacaaccttg ggtggttgat ggtctcaacg
      181 gatttgaaaa atggatcggc tactttgcaa ttgacgagaa tgaacgctat gaaggccgca
      241 cgcagcggat tgatcgaatc cattttcttg accgaacatt gtatattgac ggtccaacca
      301 accggtacac cccgggctat caccccggac cggccgaacc ccagcttcgc agtcttttct
      361 atctgccgga tggcccaatg ccgtccaccg actggataca aacttcaccc aataacaacc
      421 ccggactctg gcaaatcagc agcggacaag ctcttcaaag cagccaatct tgtttcagct
      481 ttaatcttgt caaccgcgac gcggcaactt gttatctttt tgaagccaat gtgacaatgc
      541 cgcagccacg cgacagcgaa gataaagccg gcgtggtggc ctactataaa aactcgacca
      601 actgggtaat catcggtctg gaccgaagtc tgggctacgg agccgacaat tggtactgcc
      661 atatccgtac agatgcatat aacggtgtcg ttgccgccgg cggattcggg ggccgcctca
      721 actacggcgt ttatcataaa atacgcgtcg agcgaaacag cacgcttttc agggtctgga
      781 ttgacgatat gctgccgccg ggcttttcga atattcaaac atccatcact gacgccggcg
      841 tgccgggcct ttactctgat catgcggccg cactatttga cggcattatc tatacaatcg
      901 gctgggatga gtacggcagc aacattaccg gctggggcaa cggccttaac ggcagcaggc
      961 aaatcggcac ctggacagtt ggtgccgacg gcattgccgt aacaggcggc ggaactatct
     1021 ataaaggcga tcttatggaa gcgtatgagt tttccacgca tctgtataaa accggcattg
     1081 ccgagaatcg gatgggtatt tatgctgccg caatcgatgc gaataacttt ctccgggcct
     1141 atttcgatct gcctgctgac acattcgttg tggacggaaa acaaaacgga cagcatctaa
     1201 gcccgcaaat cataaatatt cccaatcagc aggattataa tctgcgagct gtgaagctgc
     1261 ccgatcgagt gatatttttt gtcgacggac gagaagtctt aagccgctct atctgttttc
     1321 ctgcatccca gacagcgctg tttgcagaca atatgagtgc ccgcttcaac gggattcttg
     1381 cttaccgcat cgaagcagaa acgctgccgt cttcatggaa gcatacggat gtaggctctg
     1441 tcggttttaa aggcagtgca agctataatg aaggaacttt tactgtaaac ggttccggca
     1501 gcgatatctg gcttatacag gatggttttc attttgtcta tcaggatctt tacggtgacg
     1561 gggaaatcat tgcgagggtg gtttccaatg acatcacaga ctggtggaat aaagccgcag
     1621 ttatgattcg ggacggtctt aacagcagtg caggcatggc gctggtaacg ctttgcggtg
     1681 ggggaaatct gcagtttctc tggcgcacag gtcccggagt taatgcccaa ggcatcgatg
     1741 ctcccaatcc tccctatccg cagggtgtct ggcttcggct taaacggacc ggccgacagt
     1801 acgacggcta ttattcttat aatgggtcaa actggacatt gatcggctcc cgtacactta
     1861 atttcagcag tcatctccag attggactgg cggtaaccgc acatcataac aaccggctga
     1921 acggagccgt atttgataat gttgctattg gcggatgtac gcccggtgaa taccaaactg
     1981 acctgaatcg ggattgcacc atcgatataa gcgatctact ggtgctggcc ggacaatggc
     2041 tggacacagt ctcagcaggc acgtctgcag atttggacgg caatttgaaa atcacccttg
     2101 gcgattatgc gattttagca agcgactggc tgattgatta cagatattaa acagcatctg
     2161 tcttttgatt ctgctgtttt ctgctgtatt tttaaaaact tttaccagca gttctgctga
     2221 aaatcacctg cttttcaaaa tatctttatt cgtctggcgg attagcgtgt atgaatggtt
     2281 atcttttccc ggtcaagccc ttcggcttga gcgtgcagca caatatctcc agcgccgcct
     2341 ctgggtcgaa ggatagccaa acaccgcccc tgaaatgttt tgcgctgcgg gcttttaaaa
     2401 cttgccggtt catttggaac tccacttccc tgtgcagcaa gctcccctgc acccataaca
     2461 gaaaagtgaa caagaacctc cgcatccggg acacgctggc cgtctgcgtc aaccacttca
     2521 acggttacat aggcaaggtc actgcggctg gcatggatag atgtccggtc agctgtcagc
     2581 cgcaattttt tcggggggcc ggcggtttta agcgtcgctt ttgctgcaac ttttccgctg
     2641 attaatccca gagcacacaa ttcgccggga gcgtacggca cacggaattg ggctgtcaat
     2701 gttggagaaa caggctgctg accgactact tttccattga gctcgagccg gaccgtttcg
     2761 cagcgtgaat aaaccgttat ttgcagaggt ctcccttcct gtcccggcca gttccagctt
     2821 tgcaattcat ccggccaagc ccaccagctg agtttttcag ccagtccagg cgggataggt
     2881 ctgtgcacaa atatttctat cggactgcga cgccacacta catctcgata gaacgattgc
     2941 ggcttcttga atccgcaaat gtccaaatcc ccgcaccagg cattgaacca cggccatgac
     3001 ataaaaaaac tctccggctg gctgctcagc accgcatgag caaggcctgc ctcgcccaga
     3061 taatccatcc ccgaccagac aaaatcaccg attacatagg gatgctcctc gacaagcgac
     3121 cagtaaagga gggcctcttt cgggaatgat tctgagccgt acattacccg ctggggataa
     3181 cgaacatggt ctgattcata tttatccggc aaataattgt agccggcgac atccagatgc
     3241 ttgaaggcaa gcccggaaag ctcatcccag ctgcgggcaa ctttgcccca atctgaacag
     3301 atcgcctggg tcacaggccg ggttgtatcg tgcagtaaaa ccgcttgacg caggcgcttg
     3361 gccgtttcct catcgcggaa ttgctcaggg atttcgttgc caattgacca cataataacc
     3421 gagggatgat tgcggtcccg ccggaccatc gaggcgatat cctgctgatg ccagtcgctg
     3481 aaaaaacgct gatacccttc ggttctattc tctttggctt cattccattg atcgaatgcc
     3541 tcaacaatca ccagcatgcc cagccggtcg catgcttcca gaaaggcaga agaaggcgga
     3601 ttatggctgc agcgaacagc attaaagccg ttggccttca ttaattcaac acgccgttct
     3661 tctgcgcggt cgatggccgc cgccccgagc ggcccattgt catgatgcag attggcaccc
     3721 ttgagaagaa ctgtccggcc gttcagccga aagccattgt gggcgtcaaa ttcaatcgtt
     3781 cgaatgccaa acgggacaac aacggcatcg gaaatcttac cggcagtgac cacttcaatt
     3841 tttgcagtgt acagattcgg ggtctccggc gaccagaggc ggggggatgg cacgtcaata
     3901 catatctccg cggaatgtgt ttggacggcc gacaggctca gcgctgtttc tacgcggcct
     3961 gccaaacggc catctgcatc ctgcagctct gttcgcacaa ccgcctgtgt cgggacagcg
     4021 gcagcattgt acacctcaac cgctgctttt accaacgctt tgtctgcgga tacctcaggc
     4081 gttgtaataa atacaccccc cgtcggcaca tgaatcaact cgttaatcag gagctgcaca
     4141 tggcggtaaa tgcctgatcc ggaataccag cggctgtttt tgccttcatt gcggacccga
     4201 acagccagca cattgtcctg cccaggggcg ttcagacagc gcgtcagatc gaactcaaaa
     4261 ctcgtatagc cgtgcggatg cccgccgagc cttcggccgt tgagccagac ttcggcgttc
     4321 atataaacac catcaaaaag cacagaaacc tgtttgttcg gctgctcgac tgtaaaatgc
     4381 ttgcgatacc agccgatgcc gccgacagta tagccggtaa aatgaccgcc ggcactgaga
     4441 accggatcaa aaggaccgat gcgaacagat ttggaacccg cctgatagat accgccgctg
     4501 ccgctgccgt caaacacccg aaccgctacg acatcggtgc cgtcgccgcg caccaataac
     4561 gctggaacgc ggtagcgccg ctggattgtc caggcggtct gataattggg cggaaaggag
     4621 cctgtaccgc cgatccgcca gccgttgaac catacttcat cgacatcatc aatacagccc
     4681 agcagcaggt caaaatcctt ccctttgcaa ttatcaggaa tttccagatg ccggcggaac
     4741 cagccgaaaa cattgtcggc ggtatgatcc gaatgatgtt cccatgtatc gggaagcaaa
     4801 accaactgcc aatcgctgtc gttcagctgt cgggctttcc aatctgcatc atctcctttg
     4861 cgaaaacgcc ataggccggt gatagcttcc aattctggaa tagatccgtc tgcaggcggc
     4921 agatcctcga tactccaatc atggggcaga tccagaactg gccaagatgt atcatcatag
     4981 tccggctgct gtgctccggc cgcatcgccg cgaaagaaac gccagtctga atcgaacggc
     5041 cggtcttgga aactgctttc aactttttgc gtactttcat ccggtcccca ggattccgaa
     5101 gaaaggaata cgccgcacgc agccagcaca acaacaagta ttcccaataa tgcaatctgc
     5161 caaaaacagt tctttttcat aacggccttt attcattttc tcacagccaa gctgcattta
     5221 aaacttatcg tcagcggata gcaaatcaga catttttatc cggctgcgat tttcggcagc
     5281 tcatttatca gcgtatcttc gataatgtgt agcagcgtgt ctagcgtttg gacatctatc
     5341 cccaccggcg gcatcagaac aatcacatcg cccagcgggc gcatcatagc ccccttggga
     5401 cgcatagcag aacagacttt ggcaccaagc cgcagcgggt acgggaatgg ttctttggta
     5461 ttcttatcct ttaccagctc aatgccggcc atcatcccgc attgtcgaac atcgcctaca
     5521 taaggcagta ttgcagcccg tgggagaaaa cgggaaataa gcgtaatctt ttccggcagc
     5581 gtctccaaaa tacggtgttc ttcaaacaac tccagcgaag ccagcgctgc tgcgcagccc
     5641 agcgcgttgc ccgtataggt atggccgtga taaaaggttt tgcaaatgtc gccgcataca
     5701 cagaaagcat caaagatttt ttgcgttgtc agcgttgctg ccagcggcag atagccggca
     5761 ctgatgcctt tggcaataca catcaggtca ggatgcacat cctcctgctc gcaggcaaac
     5821 attctgcccg ttcggccgaa tccggtagcc acttcatcgg caatcagaag ggtatcgtat
     5881 tttctggcta gtgtttcggc gccttttaag aagcctttgg ggtgcacaag gatgcccgca
     5941 gccccctgca ccagcggctc aacaacaatg gctgcgattc ggccggcgtg ttctttgaga
     6001 agaaactcca ttttttctaa gctggatgcg acacattgtt cagctgtgcc gtcaaaacgg
     6061 tacggatggg ggctggggac atagtatgtt tcaaacagca acgatttgaa aatgccgtga
     6121 aacagctcaa tcccgccgat gctgacagaa ccgaccgtat cgccgtggta ggcttcttcg
     6181 agggcgatga atttcgttcg gcccttctga ccgatattct gccagaactg ataggcaatt
     6241 ttgacggcga tttcaacggc cgtcgcaccg ctgtcggagt aaaaaacctt cgtcagtcct
     6301 ttgggggcaa tctgtatcag cttttcggct aattcaatag atgccgtctg cccaagccca
     6361 agcagcgtgc tgtgggcaat tttgtctaat tgggaacgga tggcatcatc aatttttcgg
     6421 acacggtgtc cgtgcacatt gcaccatagg gagcttacgc cgtcaatata gcggcggccc
     6481 tcggtatcaa tcagataaaa accctcgccg gattcgatga ttaccggctc acaggcaagc
     6541 caatcggcca tctgtgtaaa cggatgccac agatagcgtt tgtccagttc tataagctgt
     6601 ttcgttctgt tgttcacaaa ataccattat gtcaaaaatc cgcaactcgt caagaataag
     6661 agccgataag tgcttgcaga ttcagcggct taattttaaa ctggttttgc agagcaacta
     6721 tttttatcgg agcaaaacaa tgtctatcga aaagatatgc ttgttctttt ttcttgccgc
     6781 ctccccgctt tgggctggat attcgctgtt cgtcagtccc accggagatg ataccgcagc
     6841 cggtacaagt cctgaaaatc ccataaaaac ccttcagaag tctatcgatc tggcaaggca
     6901 aattaaaaac cagcccgtaa tagtttacct caaaggcggt gattacgaag tcacaaaacc
     6961 gctgactttc ggtatggacg attcccgaac tgccgacaat ccgctgacct tcaagccctt
     7021 tgaaaacgaa aagcccattg tcttcggagg ccggcgtatc cgcaactggc aaaaacagcc
     7081 ctctggtatt tggactgcgc aaattgatga agttaaaaac ggtcaatggt atttttcgca
     7141 gttattcatc aatgggcagc ggcgccgccg tgcccgcatc ccaaacgaag gatttttgcg
     7201 cgtagccggt tttcccgacg gcggacgaga tgtgcattac cacacagact gccagcggtt
     7261 cgaatacaag cccggcgata ttgatccaaa ctgggtaaat atggaagatg tcgaggttat
     7321 tgtatatcat ttctggacag attcgcacct gccgattgct tccattgatc ccaacacaca
     7381 catagtaacc ttcaagcata aagccggcaa aatctttaca gatgacttta ccagcactgg
     7441 ggcgcgctat attgtcgaga atgtctatga agggcttgat gccccgggag aatggtattt
     7501 agaccgcaaa acaggtgtcc tatcctgcat tcccatgccc ggggaggata tagccgaagc
     7561 gattgcgccg gttgcaccag agcttgtgcg aatagaagga agtcccagtg atatgcggta
     7621 tagtgagcat atccgttttg aaggaattga gtttgcgtat acccattggc tcttgcctcc
     7681 gggcaacagc aatgatgcac agggagcttc cagtgtgccg gctgccatca cgctgacggg
     7741 ctgccggtct atctgttttg accgctgcac cgtgcgtgat ctcggcacat tcgcttttga
     7801 tattggcgcc ggctgccgcg aaatcaccat cagccgatgt acgctgaaga atctcggcgg
     7861 cgggggcatt cgcatcagcg gcggcagctt cagcagtccg ccgctgctcc ggaccggccg
     7921 taatgtcata gcggataatc acattggacc ctatggcgag gtatatcctt cagctgtcgg
     7981 cgtactgctg atgcatacct tcggcaacac catctgtcac aatgagattc atcacggctg
     8041 gtacacgggc gtttcggtcg ggtgggaatg gggctatcag ccaagcatca gcagagataa
     8101 tatcatcgaa tacaaccata ttcatcatat tggacagggg ctgctgtcag atatgggagc
     8161 gatttacaca ttgggcgtat ccccgggtac aatcatccgc aacaacttga ttcacgatat
     8221 tgattccaac cactacggcg gctggggtat ttacaacgac gaaggatcca gccatattct
     8281 ggtagaaaac aatatcgttt ataacaccaa atttgccggc tataacatcc actacgccaa
     8341 ggaaatcatc gtacgcaata acatctttgc atacggacgg tttcagcagc tcagccgcgg
     8401 ccgtatggag ccgcatcgca gctgcttttt tgaaaataat attgtgtatt ttaccgaagg
     8461 tgccctgctg gacggcaatt ggaaagatgc atcctatgta ttctattaca atcccaatga
     8521 acccaaaggc agagaaacat taagcacttt tgagatcgat tggaacttgt attacaatcc
     8581 tgctttgccg ctggaaaagg tttccttcaa cggccagacc tttgccgact ggcaaaaacg
     8641 cgggaaagac aggcactccc tttatgctga cccgctgttt gtcaatccgg acagttttga
     8701 ctttcgtctc aaacaagatt cgccggcgat gaagctgggc tttgtaccga ttgatacttc
     8761 aactgtcggc ccgcgtccct gaaaggccat tacgaataca gactgcctat ttgacagggc
     8821 acagttccat tgggtcgccg aaaaatttct cgacggccag accttccaga aggaatttct
     8881 ggagattttc tgctctatcg gtcagcacca ctcggtcatc ttcaaccacc tcatgtttga
     8941 taagctgcgg cttttcttta agcagcttgc cgacttcatc cggattcatc gccgccagtt
     9001 taaacgactc agctgtcttt tcgactttga aaaacagatg cacaggcagt atatgcagct
     9061 ttagccactc gccggcttca agctctgcat ccgtagcggg gtaaaagtcg aaaaaacgct
     9121 ttccgctaac ttcaaccaga cgagcaatca gcttgctttg tctgccttcc ttttcgctga
     9181 ttgtgagagt ataggacttt tcttgaggat tgccgacaaa tacccatgtg gactcatctt
     9241 gtttgtatgt gccggctatg gtatcatcga aaatcagcgt tttatctgtc cacaattgat
     9301 gcagcgaagg aacacagccg gctagaatcc ctaataaact cgcggaaaga agcagatatg
     9361 acgttttcat agcatttctc cctaaactat ccagcaaacg ccatttcaga taacaagaac
     9421 ctaccacatt tcttctatta tattatgctc tgggggcttt gggacaaaaa aaatcaggat
     9481 acttttgcag gcatttgctt ggcccgcgca atccaatcag gattttcaaa aaattctttc
     9541 agatccaaat attctttttt aaccgcagca aacagttccc cgacattgtc taattttttc
     9601 tcagcagcag ctttttccag ccgccaagcc tttttagcaa gggcaatagc ccccatattg
     9661 gatgccgatc cttttaacgc atgtgcatag agttcaatct gctcgggact tatgcttgca
     9721 acagcacttt tgagtgaaag catcaaggtc aagctgttct tgagaaaagc accagcaaac
     9781 tcctcaatga tggattcatc accaatccgc ccgtagacgt cgtcccagtt cagcggacat
     9841 ttagcatcat ctattgtatc ttcattataa ctcataagtt ctcgtcttct gctttatcgg
     9901 acatttttca tataaaataa agtggccatt tatatttcga ccaaatcagg gtaaaacatt
     9961 attggcctga ggagattttc attgcatctt aaattaaata agcacaatat aaatttctgt
    10021 tatggtcagt tttgatgaaa aaaaccgcta tgaaatggca cgacgagcaa tggtcgattc
    10081 agacttgcgg ggtcgggata taaacgacag ccgtgtactg aaggttatgg ccgaaatacc
    10141 ccgcgaggcc tttgtacctg caaaatatgt aggccagagc tatgcggact atcctttgcc
    10201 tatcggaatg gggcagacca tcagtcaacc ttatatggtg gctctgatga cccagtgtct
    10261 tgaactttct ggaaccgaag aggtcttgga aatcggaacc ggcagcggct atcagacagc
    10321 aattctgtca aagctggccg ctcgggttta taccatcgag cgattctgcg aactggctca
    10381 atcggctcaa actgtattat ccaatcttgg cctaactaat attgaatatt atatcggtga
    10441 cggcagcacc ggctggcccg agcctcggca ttttgatcgc attatcgtta ctgcaggcgt
    10501 ccccgaagtc ccgcccccgc tgattgatca gctcaaagat ggcggtctgc tgacagcccc
    10561 tattggcgga cagtggtctc aggaacttat tgtgggccga aagcagaata accagctgac
    10621 tactcgtacg gtgtgcggct gccgatttgt tccgctgata ggcagatacg gctattccga
    10681 accaaaagaa atgagataaa agaagatagc caattaccat ataaaaattt ttgtgatcat
    10741 tgttgaaact tctcagagat ttcaactgct gaaatgccgg ttaaattctg acaaatatgg
    10801 atagagatat attgtaaaca taataagatt gtaccaatac tgcttatata gccgaaaatt
    10861 ccattgaaac cgaaaaaaac tgccataatg ggtccgaacc atctgttgca gctgaaatta
    10921 gcttattgcc tgccgagact ttggatgtta taatttgacc ttctaattta aacaagacct
    10981 ttgaggtgca gaacaatgac attagatgtc actgaacaag tcaaactgct caaacgcggt
    11041 acggtagaag tgtatcgtga ggatgaactg gctcagcgct tagcagaagc agccaaagaa
    11101 ggccggcagc ttcgggcaaa actggggatg gacccaacgg cgccggacat tcatctgggg
    11161 cacacggtag tacttcgtaa gctaaggcaa tttcaggact tagggcataa agccgtattg
    11221 attatcggcg attacaccgc ccgcatcggc gatcctactg ggcaaaacgc aacccggccg
    11281 atgctgtctc ctgaacagat tgagcaaaac gcaacaacct atttccagca ggccggcaag
    11341 attctggata tgtcccctga caaggttgaa gtgaggttca acagcgaatg gctggccgat
    11401 ttacggttgg cggacatcat caagctgaca gcatccatga cggtggctcg tatgctggag
    11461 cgggacactt tcgagattcg ctataaaaaa ggggatccca tcggcattca cgaatttctc
    11521 tatcccctga tgcagggata tgattcggta gcaatcaaaa gcgatgtcga attaggaggc
    11581 accgaccaga cctataataa tctggtaggt cgggacttgc agaagcttga tggtcagaag
    11641 ccgcagatcg ttattacaat gccgattctg gtcggtctgg acggcgtgca gaagatgagc
    11701 aaatccaagg gcaactatat cggcgtaact gacagcccgg ctgatatgtt cggcaagacg
    11761 atgagcattc ccgacaatct gatggaaaac tattatacgc tgttgaccga tataccggtt
    11821 gatgaaatta aggttctttg cgatcctcaa caaacccacc cgaaagaagc caaggttaaa
    11881 ctgggcaaac tgattgtcca gcaattttat gatgctccaa cagctcaaaa ggccgctgag
    11941 gaatttgaca agattttcgc ccaaaatcag cttcctgagc aaatgcccga ggtggaaatc
    12001 ccggcagaac caattatgat cagcaaactc ttggtgcagt gcggcttaac agccaccggc
    12061 ggggaagcga ggcggatgtg cattcagggc ggggtgcgga ttgatgacca gcgttatccc
    12121 gaccctgccg ctgtggtaac acctgtcagc gggatggttg ttcaggtcgg caagcgaaaa
    12181 ttcgccaaat taaaaattat agagcagccg atttgacaga caaacaataa acttgcaatg
    12241 tcagcaattt ggcagtttgg agaatatgat gaaattgcct caattaactg agccagataa
    12301 atataccggt ttttatgcag tggacttcgg tgaccatagc ggcgtaggat ttacggcaga
    12361 agaagtagcc gaactgcttg aaagcgagaa gtttagcgat ataaaggttt atcgcatctg
    12421 ccgtgcctat ccagatggaa cattagaatt gaagggcgtt cgccgcgaaa ccttccagat
    12481 ggaagcgggt atgttcttct acgccgccga tgaagaacag gcaaggatgg attttgaccg
    12541 cttgcttgac tgggccaacc gccagccggc gccttgccgt gccaaagtgc atctggctcg
    12601 tttgggcaat agtttcgtca cagccctcat ctatccggca gaatatgatg atgatatcag
    12661 ccgctggctg ctggatggtc attaccgcac atgcggtgca gcggaaggcg gtacaggggc
    12721 agttaatcga tattatcagg ccgattctca agtgctggac cggcggcaat tatggccggc
    12781 gtcgtcgatt gagcatctcc aaggccaagc ccttctggaa gcggctaaac gagcgatagt
    12841 acgatgaaat ccgtttgttc aggcaggcat cgtcatctgc aaaaacattt gggctggctt
    12901 gcagggcttt tcctgcagcg gttcggccga aaaccgcccg aaaaaaacgc cgcacacatg
    12961 tataaacagg acttttccac aagcacacag cggctggggc tgcgttttac tgaccgcctg
    13021 cgggatgtgt ttcgccggca ttggctgcgg ctcaaataaa ttcataatgg cggcctatca
    13081 ggagtaattt tcttgaaata cagagcagat gccggtaaag tatgctcatc tgaccataaa
    13141 attcaccgat tggagcttag cggtcaacca acgggagagg tgtcggagcg gctgatcgag
    13201 ctggtttcga aaaccagtgt gcgcgtaagc gtaccgcggg ttcgaatccc gccctctccg
    13261 gtttttcaac atagtttttg atctatcatc aataatcgcc gcaaaagaaa catcaattct
    13321 atctttttta aaattgcacc gctcaacaat aagacttttc tggtattttg tgtcgtaatt
    13381 gccgcaccta ttgacaaaaa ataatgctta aaaggcataa aaaaaccggc ctttgcttga
    13441 tagagcaaag gccggttttg ctgaagtatc tgttttacgg cagcaatccc tcaaaaatag
    13501 ggattgatac cgtcaatttt cagaggcggc tgcattttta aggattctgc tttttgcccg
    13561 gcatcgggat atttaaagag tcgggcatca acgtgccatc cagaccgccc cggcgcgggc
    13621 ttgattcgtt ttcctctatg ggagccgaag cagtcgtctg accagaagcc gccgcttccg
    13681 ctgctttatc ctcggcagcc cactgtacac ggcgcaagct caggccgatt ttgcgggaat
    13741 ccgtatccac acgcagaatc ttaacttcca gcacatcgcc gattttgacc acatcctgcg
    13801 gcgaatccac tttggaatct gacaattccg aaatatgcag cagaccttcc agatccggtt
    13861 ccagctctac aaacacgccg aaattggtca gcttggtaac aacacctttg acaatctggc
    13921 cgggaatgta tttttccggt attgcacgaa tccacggatc ctctgtcaat tgtttgacgc
    13981 ccagtgaaac ccgatgtttt tcttcatcga cttccagcac cacacatttg acttcctgcc
    14041 ctttctgcag ggcttcaccg ggatgattgt acttcttggt ccagcttagg tcagagatat
    14101 gaatcaagcc gtcaatgccc ggctcgattt ccacaaaagc accgtaattg gtcaaactgg
    14161 taactttagt ggtaaccatc gtgcccacag gatacttctg tccggctatt gtccacggat
    14221 tggtctcaag ctgcttgagt cctaaggaaa tttcctgctt ttccttgttg acatccagca
    14281 ccatcacttc aacttcttgc ccgagtgtca gaatgtctcc ggggtgggca atgcgcttgg
    14341 tccagctcat ctcgctgata tgaataagac cttcgatgcc ttcttccagc cggacaaagg
    14401 ccccgtagtt cataatattg acaatcgtgc ccttgacccg cttgccgacg gggtatcgct
    14461 gttcggcaga ttcccaagga ctgggctgct tctgcttgag tcccagtgag attttttcgt
    14521 tttctctatc gacgccgatg accatgcact cgatttcctg atccagctgc accagttcgg
    14581 acggatgaga aatccggccc cagcttaaat cggaaatatg cagcaggccg tccagcccgc
    14641 ccaaatcgac aaagactccg aaatcggcga tatttttgac aatccctttt cgaatctgac
    14701 cgacttcgat ttcccgcagg atgcgttcct tgctggcctg ccggtcttct tcgataagtt
    14761 ttcggcggga gaccacaata ttgtgatttt ccgtatcgat tttgagtatt ttgcattcaa
    14821 tttcctgacc gataaaccgg ctgatatcgc cgggcttgcg gatatcaacc tgcgaagccg
    14881 gcagaaagac cggcacaccg atatccacca gaagaccgcc cttgatgcgg cgtgtaacca
    14941 tgcctttgac aacatcgcct tccttgttgc tggcaatgac ccgctcccag ccgcgaatgc
    15001 gatcggcttt gcgtttgctc aacagaatac cgctttcggc atccatttct tccaaaagca
    15061 cttcaatttc cttgcctgga acaatctcgc tggggtcatc aaattcgccg gcatccacaa
    15121 tgccttcgct tttcagaccc agttccacga taacatcatt gcccagctgg gtaacaatct
    15181 tgcctttgac aatggtgccc ggaacaaggg cttcgacttt cttttccagt tcctcggcca
    15241 aaagctgtgt gtgttcaggg gtgaaaagtt cgcctacttc ctgctcaatc tgttcattgg
    15301 taagaccgag cttgtaaatg agatttctat caaccattga aattctcctg cggagtctgt
    15361 ttgtcggccg ctgcggcgcc ccgcatttac gccgccgacc ctgacctcaa acaaaaatgt
    15421 taaggtttat aaaaaacaca ttgactacgc cgtcaatgcc atttacgcct ctgattagat
    15481 tcagcaatcc gtttccgccg gaggcgacca ctgctcaagg tttcgcacaa actcttcaat
    15541 cacctcatct ggtgttgacg ctcctgcggt aactcctgcg gtggtcttgc cgaaaaggta
    15601 ttttttatca aattccgccc agttttgcaa atgatatgtt tgcggattat gttttttaca
    15661 taattcggcc agttttcgtg tattggcgct gtggagcccc cccaaaacaa acataatatc
    15721 aacctgccgg catagcttaa ccgctgcctg ctgacgctcc atagtttccc gacaaagtgt
    15781 atttataatc ttcatttcag taaagccgcg tcgtgcaatc gcagcaatca tatcagcaaa
    15841 atgatctgga ctttgcgtag tctggcagat aactcctatc ttctttgtta aatttaactt
    15901 atctaaatcc tcttcattac ctacaacggc tatatcctta gcatagccaa ccactgccct
    15961 tatttctggg tgattagtgt ctcctattat aacaacctta tatccttcat cgtttaataa
    16021 tttagctatt ttttgaacac gacgaactaa aacgcaggtt gcatctacaa ttttaagacc
    16081 ttttttgttt attttctcaa actcttcctg cgtagctcca tgagagcgga ttagtactgt
    16141 tccgttttga acctcatcaa tgctgtctac tggtttaagg cctgcttctg aaagctgcct
    16201 tactacatct tcattgtgga taatatgccc taagctgtag gccttgcctt cttctgccag
    16261 tgtctttctg gccagattga tggcattccg cacgcccgga caaaaaccgc atttttcagc
    16321 tactaatact ttcatcgcaa accgtttgcc agtttactcc tttttgcatc ctgattcaat
    16381 aattccctga aaaggtagga aggttttgta ccgccctgct ggaattcatt ttcttgacaa
    16441 taaagccccc ttctgcgata ctgtttgcct tgttttaccg caagcggcag gggcgttcct
    16501 gccactggag aaatccgcag aaagttcaaa accttatcaa aaagggagta ctaaatgaaa
    16561 gtctcggaga tgaaaaaagg ccagaccatt atgatggacg gcattttata tgtaatcgta
    16621 gattaccagc atgtcaaact cggcaaggga ggagcagttt atcaaacaaa acttaaaaac
    16681 ctgaccgatg ggggcatccg cgatgttcgt cttcgttcgg aagagaccgt agaagaggcg
    16741 tatctggaaa agcggaattt tgaatatctg tattcgtcgc ctaacgaaca tgtttttatg
    16801 gaccttgaaa gtttcgatca gattacactg gatgatgacg cattcggtga tggagcaatg
    16861 tatttaaaag gcaatgaaaa agtgcaaatc agcatgtaca aaggcaagcc ggttgtaatc
    16921 acgctgccca acaccgttga tttgacggtt accgataccg ccccggaaat caaaggggcc
    16981 actgccacca accaatataa gccggccaca ttggaaaccg gtgtcgtcgt gcaagtacct
    17041 ccgtttatca aggtgggtga aaaaatccgc gtggataccc gcaccggcga atatgtcacc
    17101 cgcgtaaaag attagcagag ctttatactg cattccgagg taaatacgac gggttcaata
    17161 cagaaatcga gatccaaagc aaaattaaag cgattatctg ttcaagacaa aatgatcttg
    17221 aactataata tctttgcttt tttctgcata tcaccatttg aattatagat ttgtttagaa
    17281 attgggattt cgaacttcgg cactatacac attttaaggg gcatccgatg gcattagcgg
    17341 cgttcaacaa atttctggtc ggcattttcg gatcgcgtaa tgaacgcata gtcaagtcct
    17401 atatgcagat ggcacaggca gccggaaagc tggaatccca cattcaaacc ttaactgatg
    17461 agcagctcaa agccaagaca gatgaattca agcaggcact ccgggatggc cgacggccgc
    17521 aggatatact gccggaagct tttgccgtgg ttcgagaggc cgcccggcgg cacattgcca
    17581 tgcgtcattt cgatgtgcag ctggtcggcg gcaatgttct gtatgacggc aaaatcgctg
    17641 aaatggccac cggtgaaggc aaaacgctgg tggctacact ggcggcctac ttggtgcatc
    17701 tgacagggcg caaggtgcat atcgttacgg tcaatgatta cttagccaaa cgcgatgctg
    17761 aatggatggg gccggcctat cgggcgctgg ggctgtccgt cggggctatt caggcagaga
    17821 tggacacctc cggcgacgag cggaagcagc agtatgcctg cgatattacc tacggcacaa
    17881 acaatgagtt tgggtttgac tatctgcgcg acaatatgaa aatttctctg tcccagatgg
    17941 cacagggacc gctggagtac gcgattattg acgaagtgga ctccatcctg attgatgagg
    18001 cccgcacgcc gctgattatt tccggtccag cagtggacga tgtcagccgc tacaaaaaag
    18061 ccgacagcgt cgcccggcaa ttgatcagct tgcaggccgc ttataacaat ctcaaaaaag
    18121 ccatcgagac cgccgaacgc acactggcta acgctca
//
LOCUS       DAOTBO010000055        18053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2572_length_18053_cov_5.789254, whole genome shotgun sequence.
ACCESSION   DAOTBO010000055 DAOTBO010000000
VERSION     DAOTBO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18053
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_18053_cov_5.789254"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1138
                     /gene="mutY"
                     /locus_tag="PLE88_07625"
     CDS             56..1138
                     /gene="mutY"
                     /locus_tag="PLE88_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOM76370.1"
                     /translation="MKQTQANEIQNYLLLWFSAHARDLPWRRDRRPYSVWISEIMLQQ
                     TQVAAVIDYYNRFMKRFPTLESLAAAKLDSVLKLWEGLGYYSRCRNLHKAAKTIVTHY
                     GGRLPDTVADLQKLPGIGRYTAGAIASIAFNRSAPILDGNVIRVLCRVFRINSNPKET
                     ATKKRLWQLADSLVHTKEPGRFNEAMMELGATVCTPANPQCPACPLRQYCGAFAHNEQ
                     NELPIKHKQPPLPHYTIVAGIVFKDTQILIDKRRPDGFLGGLWEFPGGKKQKNESFKS
                     AVAREVKEETGIDIEVGTRLCIVRHAYSHFKITLHTYLCRYKSGTARPLACDAVKWIA
                     PKDLKKYALPAANQKIISCLKSAGIL"
     gene            1229..2029
                     /gene="dapB"
                     /locus_tag="PLE88_07630"
     CDS             1229..2029
                     /gene="dapB"
                     /locus_tag="PLE88_07630"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOM76371.1"
                     /translation="MKPALAINGAAGRMGKRLIALAAEQGIFDITGAAESDQHPDLGK
                     DAGLLAGIKQLNVPLSSSLPDSADIVIDFSQPQAAEKSIDYCVKNGTALVMGTTGLSA
                     AQLARLKEAAQKIAVVQATNMSLGMNLLFAIVGRVAKALGQEYDIEIIEKHHRFKKDA
                     PSGSALSLAEAICRETGCAYPDCLIHGRQGKEALRQKGTIGMHAVRAGDIIGQHTVIY
                     STLGETITIEHCAHTRDTFAQGALRAAVWLINQKPGLYTMLDVLGLND"
     gene            2096..3202
                     /locus_tag="PLE88_07635"
     CDS             2096..3202
                     /locus_tag="PLE88_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOM76372.1"
                     /translation="MVKLETLIAAGEKYEASDIHLTTDQPPRLRIKGDLKTIGGEGAT
                     LTEENIMQILEQTLHVRQKERCRQLLQENGSVDYSYEVSLNSKPIRYRVQAYYSMGKP
                     AVAMRRIRYEIPTFEQLHLPPIYKTVMENPEQKGIIIVGGETGSGKSSTLAAMIGYLS
                     RHTERHFVTIEDPVEFVFRDTKTLIHQRELGLDFPNYAEALRAVLREDPDVIMIGEMR
                     DADTVHAAIKAAETGHLVLTSLHTATAAWTFSRILNFFKEAEHDAVRLNLSYNLLAIM
                     NQMLIPSEQKGIGLIPATEVFINTPSVRQYLKEKEKESQLADVIKEGHDGMHSFNMSL
                     ARLISEGHIGVTQAKSYSHNPQELEMLVKVTKTK"
     gene            3321..4022
                     /locus_tag="PLE88_07640"
     CDS             3321..4022
                     /locus_tag="PLE88_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOM76373.1"
                     /translation="MTQYGLPQAAVYPAVLIAAMAGYYILANRLLQPACACPAGILLV
                     WLPEVMLFAVLIWVLSFFRDPPRTIVQDARLLLSPADGTVMAVEELPGFEGFDGPVLR
                     VEIFLSIFNVHINRMPCRVQVGTIHYKPGQFLDARNPQCSKVNEANEIELFRLDDPKD
                     RLLVRQISGAIARRIVCDAATGRQYPAGEKFGMIKFGSCTELYVPARSNLVCMVKKGD
                     KVKAGLTIIARYQEC"
     gene            4016..4936
                     /locus_tag="PLE88_07645"
     CDS             4016..4936
                     /locus_tag="PLE88_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HOM76374.1"
                     /translation="MLIRNSQQSVRSKGRFFRSHRHRLKTIALLPSTITLINGICGFA
                     AIGLAAKGPRHYALAAYMIFYAMIADMLDGRVARISKTTSSFGGQLDSLCDVLSFGTA
                     PAFLMLNLLLYHHRQLVGSAQLILGDLFERFIWITAVVYLSCAAIRLARFNVENEEDE
                     TAHMSFSGLPTPGAAGVVASIVMFSQSLLTDAAAQTVLYQMLYNGILYILPFVTLACA
                     LLMVGRLRYLHLFNHLFRGRKPLNYLYFTIFSVAMIALCGLELSLVLTFTGFAASGPI
                     RWLWRQILLPKLGHKTAEAAEPVHTTAPLN"
     gene            4979..6112
                     /gene="hemW"
                     /locus_tag="PLE88_07650"
     CDS             4979..6112
                     /gene="hemW"
                     /locus_tag="PLE88_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOM76375.1"
                     /translation="MLPQTATVGLYVHIPYCIKKCPYCAFYSEPIEQADCRRFSAALL
                     NELRQQSIAESAETIYIGGGSPSSLPPEVLSGLASELHKRFHSVQEFTVECNPAQADE
                     TLLRRLRSTGVSRISIGAQSFHSQELEVLGRIHSPKQIADAVRAAQTAGFNNIGLDLI
                     FGIPGSTIENWTKTLQAAMDLNVQHIAAYSLTIEPGTPFEQAIQKGTLALIDEQTERR
                     MYETAQSMLAEAGFVQYEISNFAKPGFECRHNIRYWKNLPVVGIGPAAASWYKGLRKT
                     NTADVNRYISAVEAEQPAYTQYERPSPEQTARETAVLNLRMIEGINLAEFHRQTGFDA
                     IALFGKSIAVHLESGLLEQTATHQRLSKKGLSYADTVAEDIIL"
     gene            6210..6986
                     /locus_tag="PLE88_07655"
     CDS             6210..6986
                     /locus_tag="PLE88_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOM76376.1"
                     /translation="MELIDTHAHLTYDGLADRIDDVLARSIQAGVVGWIAVGTAQDEN
                     NKVIDLASRYEALWAAVGFHPHSADNVSRADMDRLAKQLQHEKAVAVGETGLDYYYMH
                     SAAENQKRIFRAHLAIAAVLQKPVVVHTREAFDDTIEILDEFADRIKKVVIHCYGGDA
                     EQTKQILKRGYYVSFTGTITFKRNDTLRQIIKDLVPLDRVMIETDCPFISPEPMRSVR
                     PNEPALLIHTAQCLADLYNLPLTDFANQTTKNSRLFFGLY"
     gene            7627..7797
                     /locus_tag="PLE88_07660"
     CDS             7627..7797
                     /locus_tag="PLE88_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76377.1"
                     /translation="MHIYQDSQENMPQPFYIKPFEVVPREIELYGTFKVQQTADYFWL
                     AHRRNRGAYRLE"
     gene            7985..9025
                     /gene="queA"
                     /locus_tag="PLE88_07665"
     CDS             7985..9025
                     /gene="queA"
                     /locus_tag="PLE88_07665"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOM76378.1"
                     /translation="MKTEELNYTLPEELIAQHPSQRRSQSRLLVLHRAAGTLEDRQFA
                     DLPEYLRPGDCLVLNDTKVVPARFFIQKSTGTRLEGLFLHQEKAGHWQILLKNARRIK
                     SGQTVDLLDRAGKSWCKAVIRDGDVPEQRCLVLDKPQDAFFVLDNIGLPPLPPYIKRS
                     SSIPEDLNRYQTVYARRLGAVAAPTAGLHFDPPLLEKIGQNGIRIAMVTLHVGMGTFR
                     PIQTETLEEHPMHEEWFEISDTAADVINQTIQSGGRIIAVGTTSVRTLESTADGRRIR
                     SAQGHTRLFIRPGYTFKIVDAMITNFHLPKSTLLALVGAFAGLDRILYAYQHAIKQQY
                     RFYSYGDAMLIC"
     gene            complement(9104..9559)
                     /locus_tag="PLE88_07670"
     CDS             complement(9104..9559)
                     /locus_tag="PLE88_07670"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOM76379.1"
                     /translation="MHLDTQFSIFMVNKPGVLAQLLNVLAAEKINIVAMTMMDSVEHG
                     VLRLVGSGSDKIRSILQRMNMQFSETQVLCVNLPNHAGAFADVTNKLAKAHINISYAY
                     CTAGARGGRTTAVLKVADVKKSMKVLEGHAETDNQKTKKSVRRSLASRR"
     gene            complement(9632..10012)
                     /locus_tag="PLE88_07675"
     CDS             complement(9632..10012)
                     /locus_tag="PLE88_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM76380.1"
                     /translation="MNQRNDNELLDITTREDIWIVLFRQSSIGSMGEIEKIADILRAL
                     VCKQHPAKMIIDFSNVRFFSSQMLGLLVDLWRRMKEAGGKLLICGINPQLIRVFRITH
                     LDKLFEFYENSAAAAAALQLQGIQ"
     gene            complement(10082..11098)
                     /locus_tag="PLE88_07680"
     CDS             complement(10082..11098)
                     /locus_tag="PLE88_07680"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOM76381.1"
                     /translation="MKEPSKTLVEVKDLKTWFPIRAGIFSRTVGWVKAVDGVSFSIRQ
                     GQTLGLVGESGCGKTTVGRTLMRLIPPTQGTFIFDQMDVFSLSAGEMKNFRQNVQMMF
                     QDPFGSLNPRMTVENIIGEPLIVHRSMSAKQRRQRIVELMEQVGLSAQHLNRYPHEFS
                     GGQRQRIGVARALALNPKFLICDEPVSALDVSIQSQIINLLQDLQSRLNLTYLFIAHD
                     LAVVEHISDWVAVMYLGHIVEYANRDTLYADPLHPYTVSLLSAIPEPDPSRQKKRIVL
                     KGEVPSPSNPPSGCPFHPRCPMAEKRCAEETQQLEAKDQQGQHWAACWKTSPDRIGQM
                     RSAV"
     gene            complement(11131..12150)
                     /locus_tag="PLE88_07685"
     CDS             complement(11131..12150)
                     /locus_tag="PLE88_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM76382.1"
                     /translation="MSENNNILLSVRNLTTHFHTEQGLVKAVQDVSFDILAGKTLAVV
                     GESGCGKSVTALSIMRLIPWPPGRIVEGRIVFKGESLLDAAESRMRQIRGNDIAMIFQ
                     EPMTSLNPVFTVGNQIVEAVRLHQKKSGSEAWDIAVDMMRKVGIADPQRRVREYPHQM
                     SGGMRQRVMIAMALCCWPSLLIADEPTTALDVTIQAQILDLLRRMRQESGMSIMLITH
                     DLGVVAENADDIVVMYASRVVETACAKELFEKPLHPYTQGLLRSLPRLGARKHRLDVI
                     GGAVPNPLHFPAGCKFHPRCPVGCKDTRCRTAEPPLREVSPGRCAACWYAEGYDSSFK
                     GRNST"
     gene            complement(12224..13207)
                     /locus_tag="PLE88_07690"
     CDS             complement(12224..13207)
                     /locus_tag="PLE88_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM76383.1"
                     /translation="MNNSQPTAVSADQPVKGRSLWADGMRRLRKRRLAMVCFGVVMVY
                     VLLTVLVYLSEAAGWKIPLFDWRQAVGPSYQKPWTVYAWMPEGQTQAQRGWTFFGTDF
                     LGQSTWRKTIYGAKTSITVALFASVISILIGVPLGAAAGYFRGWIDDLIVWLYTTIGT
                     IPGIILIMAFAVVLRSQTFFGVELKGVTAVYVALGLTSWVGICRLIRGEVIKRKESEY
                     VLAARALGCSNGRIIFKHLLPNVFHVIIIDFSLRFVSFIHAEVILSFLGLGETDKPSW
                     GAMIDAARTELARNVWWEMAAATAAIFVLSLALNIFGDALRDCLDPKLRTD"
     gene            complement(13200..14138)
                     /locus_tag="PLE88_07695"
     CDS             complement(13200..14138)
                     /locus_tag="PLE88_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM76384.1"
                     /translation="MTAYIIRRLLYMIPIVIGVLLLTFVLFTLVGGDISQQIAGKNAD
                     AETIAEIRHEYGFDKPLFLSWDSQFLNHFKKAFTFHFGRALDRERISEKILRGVGPSL
                     ALTVPMFFGTLVISISFALVVAFLRGTVWDHIIVILCVAGMSLPYLSFIIFGQYFFAY
                     KLDWFPIFYAPDRPMVSSIVLPVLIGIAAGLGSNLRFYRTIMLDEIRSDYVRTAFAKG
                     LTARRVLFKHVLKNAMIPIVTNVVLSIPYLILGSLLLERFFGIPGLGYLMYEAVSSRD
                     FFVLNAMTFIMALLIAFFTLVADLCYAWVDPRIKFE"
     gene            complement(14135..15970)
                     /locus_tag="PLE88_07700"
     CDS             complement(14135..15970)
                     /locus_tag="PLE88_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOM76385.1"
                     /translation="MINMIKWKVKSAGFVVLAALCLLPAGCSEKGSESDEAVFRYPLP
                     VKVKSLDPGNIDDVYSNLVVSHICEPLYTYHYLKRPFEVIPLLAEALPVISPDKLTYT
                     IRIKQGVYFQDDPCFPDRKGRQLKAEDFVFAFKRIANVRYASVNWGSFKERIAGLDDF
                     REYTKQFKDEFEVDYSKEVEGLQAIDDYTLQIRLTKPWPQLLDDLTSAATAPVAKEAV
                     DYYRKDIMVHPVGTGPYRLKKWARGVFLELERNPAFRGDCYPSDGEPADAAAGLLADA
                     GKTLPFIDRIVFRIIEEEQPAWLLFLRGDMEMMGIPKDNFATAVDMQAQRPKDFLEQR
                     KIQLAISDQPSLYYIGFNMQDAVLGQNKPLRQALNYAFDSEKLNDLFYNGRWRVAHSI
                     IHPLLNEYDPNVVNEPYMGFDMQKARRLLAEAQRIQGGPIPELTIGMTGGDPFSRQFG
                     QFVQRQFESLGLKVRLDFTDWPTYVEKMNKGQHQIFGGGGVRFSTPDALNVLMMFATK
                     YFAPLGNFFFYSNPEFDRLYNQAEVMFPCAERTQLYRKMERIVLEDCPAIFTNHRVQY
                     TLYHGWIKNYKPHPYIYDTMKYIRIDTAEQKNYKRRVRELEKKKL"
     gene            complement(16018..17820)
                     /locus_tag="PLE88_07705"
     CDS             complement(16018..17820)
                     /locus_tag="PLE88_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOM76386.1"
                     /translation="MICRRVIIAAVMIAFIGSGCESKQPSAGQGEVVLNQRLEANIQT
                     LDPADVGDTNSDGVCREFYESLYGYHYLKRPHVVVPELAESLPEISQDGTEYHIRIKR
                     GIYFHDDPCFPDGKGRELKAQDFVYAIKRLADVKVQSKNWYIFDGRIVGLDAFREYTK
                     TCQKGQVDYSRTVEGLYAQDDYTLVIKLIRPWPQLIYWLAFLPTAPMAQEAVDYYGPD
                     IVHHPVGTGPFILKQWHRGVYVEAVRNPHYRRVYYPSEGADGDAEAGLLEDAGKPLPF
                     IDRVIWRIVTEDQPRWLLLMRGDIDINSIPKDNFGQAVSMSTDLTDQMKQRGMKLQLF
                     DEPCTFWLGMNMQDPVIGRNKPLRYAINYAIDRLKFIEIIQSGKGKPAYGFIPVVMNG
                     YDQSIADWSPCRFDLEKARAYMKEAEKIQGGPIPKLRLSIGRTGTVQKQTLQFLSRCL
                     MQIGIQTEIEIYDWPAFLEKLRTSDLQLYFTGWMADYPDVESFLGVFYSPNAPWPNNS
                     LFQNAEFDAIYEKVCIMPDSPERTELYRKAERIVVEQMPCAFIYHRIGYIIHHGWLGN
                     VKADPYKADTIGFGQLRYYKINAEQRDRYRKMFR"
     gene            complement(17846..>18053)
                     /locus_tag="PLE88_07710"
     CDS             complement(17846..>18053)
                     /locus_tag="PLE88_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76387.1"
                     /translation="PASRLHFRRNWTRCPKPQSLLNEHHWIHVLSCLRVNPEKADKLQ
                     SGDAAGKRSAFFLYRGLFMRLDQF"
BASE COUNT         4569 a         4927 c         4286 g         4271 t
ORIGIN      
        1 aaacctggga tgaactgccc aaggtaattg ctcatgcggc ttgctgaaac taacaatgaa
       61 acaaacgcaa gctaatgaga ttcaaaacta cttattacta tggttttccg cccatgcacg
      121 ggatctgccg tggcggcggg accgtcggcc gtactcagtc tggatttcag agattatgct
      181 ccagcagacg caagtcgctg cggtgattga ctattacaac cgctttatga agcgctttcc
      241 caccctcgaa tcccttgccg ctgccaagct ggacagcgtt ctcaaactct gggaagggct
      301 gggctattac tcccgctgcc gcaacctgca caaggccgcc aaaacaattg tcacccatta
      361 cggcggccgg ctgcccgata cagtcgctga cttgcagaaa cttcccggca tcggccgcta
      421 caccgccggc gccatcgcca gcatcgcctt caaccgttcc gccccgattc tggacggcaa
      481 cgtcatccgt gtcctgtgcc gtgtctttcg catcaacagc aatccaaaag aaaccgcaac
      541 aaaaaaacgg ctttggcagc ttgccgattc gctggtgcat acaaaagaac cggggcgctt
      601 taatgaggca atgatggaac tgggtgcaac agtttgcacg cctgccaatc cccagtgccc
      661 ggcctgtccg ctgcggcaat actgtggtgc ctttgcgcac aatgaacaaa atgagctgcc
      721 tattaaacac aagcagcccc ctttgcctca ttatacaatt gtcgccggca tcgtttttaa
      781 agacacccaa atcctcatcg acaagcgcag gcccgacggc ttcttgggcg gtctatggga
      841 attccccggc ggtaaaaaac agaaaaacga atccttcaaa tccgctgtgg cccgcgaggt
      901 taaagaggaa accggcattg acattgaagt cggcactcga ctgtgcatcg tccggcacgc
      961 ctactcacac ttcaaaatta cactgcacac ttatctgtgc cggtacaaat ccggcactgc
     1021 ccggccgctg gcctgcgacg ccgtgaaatg gatagccccc aaagacctga aaaaatatgc
     1081 tctgcctgcc gccaatcaga aaataatcag ctgcctgaaa tctgccggca ttctttaaca
     1141 gctcggtgct tgccaacagc cggcatatcg tctataatga ttatccaaaa caactgaaat
     1201 ccaatataag aaaatttagg agcgggcaat gaaaccagca ctggccataa acggcgccgc
     1261 cggacggatg ggcaaacggc tcatcgctct tgcggccgaa cagggcatct ttgatatcac
     1321 cggtgctgcc gaatctgatc agcaccctga tttgggcaag gatgcggggc ttttggcagg
     1381 catcaaacag ctcaatgtgc ctctgtcatc gtccctgccg gacagcgctg atatcgtgat
     1441 tgatttttca caaccgcagg ctgcagaaaa gtctatcgat tactgtgtca aaaatggcac
     1501 tgcactggtc atgggcacta cgggactttc ggccgcacag cttgccagac tcaaagaagc
     1561 cgcccaaaaa atcgccgtcg tacaggcgac caatatgtcg ctggggatga atctcctgtt
     1621 tgccattgtt ggcagagtag ccaaagcgct ggggcaggaa tacgatattg aaatcatcga
     1681 gaagcaccac cgcttcaaaa aggatgcccc cagcggcagc gctctatcgc tggccgaggc
     1741 catctgccga gaaaccggct gtgcctatcc ggattgtctg attcatgggc gtcagggcaa
     1801 agaggcattg cggcagaaag gaaccatcgg gatgcatgct gttcgtgccg gcgatatcat
     1861 cggccagcat accgtcattt acagtacttt gggcgaaacc atcacaatcg aacattgtgc
     1921 ccatacccgc gacacattcg cacagggggc tttgcgagcg gctgtctggc tgattaatca
     1981 aaaaccgggc ctgtatacga tgctcgatgt gctggggctg aacgattaaa ccagcccaac
     2041 agctgctgta ccggcatagg cattcgtaaa ccgacaaaaa gaaaggaacg cagtcatggt
     2101 caagttggag acattaattg cggctgggga aaaatacgag gcatcggata tccatctcac
     2161 cactgaccag ccgccgcgtc tgcgcatcaa aggagacctt aagaccatcg gaggggaagg
     2221 agctaccctg actgaagaaa atattatgca gattttggag caaaccctgc acgtgcgtca
     2281 gaaagagcga tgcaggcagc ttctgcagga aaacggttcg gttgattatt cctatgaagt
     2341 ctcccttaac agcaaaccca ttcgctaccg tgttcaggcc tattactcga tgggcaaacc
     2401 ggcggttgca atgcgtcgga ttcgctatga gattcccact ttcgaacagc tccatctgcc
     2461 ccccatctac aaaaccgtaa tggaaaaccc cgagcagaaa gggattatta ttgtcggcgg
     2521 cgagaccggc agcggcaagt cgtcgacttt ggcagcaatg atcggctatc tgagccggca
     2581 cacagaacgg cattttgtaa ccatcgaaga cccggtggaa tttgtctttc gcgacaccaa
     2641 aacactgatt caccagcggg aattagggct ggattttcca aactatgccg aggcccttcg
     2701 tgccgttctg cgggaagacc cggatgtgat tatgataggc gaaatgcggg acgccgacac
     2761 agtacatgcg gcaatcaaag cggccgaaac aggacatctg gttctgacat cgctgcatac
     2821 agccaccgcc gcttggacat tcagccgcat tctgaatttc ttcaaagaag ctgaacacga
     2881 tgccgtccgg ctgaatttaa gctataatct gctggctatt atgaaccaga tgctcattcc
     2941 gtccgagcag aaaggaatcg gcctcatccc ggcaaccgaa gtgtttatca ataccccctc
     3001 ggtccgccag tacctgaagg aaaaggaaaa agaatcgcag ctggctgatg tgattaagga
     3061 aggccatgat ggcatgcaca gcttcaatat gtcgctggca cggctcatca gcgaaggaca
     3121 catcggcgtc acgcaggcca agtcctactc ccacaatccg caggaactcg aaatgcttgt
     3181 taaggtaaca aaaaccaaat aagcgggatt gtctgcattt gctgattggg actgcaatag
     3241 agcgctggta ttcagctgtc aatcttgctg cttgcttttg agaggcaaat accgtatttt
     3301 gtaccattat gaagattcca ttgacacaat atggtctgcc tcaggcagcg gtatacccgg
     3361 cggtgctgat tgccgcaatg gctggctact atattctcgc aaatcggctg ctgcagccgg
     3421 cctgtgcttg tccagccggt attcttctgg tgtggctgcc ggaggttatg ctgtttgctg
     3481 ttcttatctg ggttttgtcg tttttccgag atcctccccg aacaattgtt caggatgccc
     3541 gcctgctgct ttcacctgcg gacggcacag tcatggccgt agaagaattg ccgggttttg
     3601 agggattcga tggaccggtc ctgcgggtag aaatcttttt aagtatcttt aatgtccaca
     3661 ttaaccgtat gccctgcaga gttcaggtcg gaaccattca ctacaagccc ggccaatttc
     3721 ttgatgcacg caatccacaa tgcagcaaag taaatgaagc caacgaaatt gaactgttcc
     3781 ggctggatga tcctaaagac cgcctgctgg tccggcagat ttcaggagca atagcccgcc
     3841 ggatagtctg cgatgccgca acaggccgac aatatcctgc cggcgaaaaa tttggaatga
     3901 ttaagttcgg ctcctgtacg gaactttacg tgcccgcccg aagcaatctg gtgtgtatgg
     3961 ttaaaaaagg cgacaaagtt aaagccggac tgactattat agcgaggtat caagaatgct
     4021 gattcgcaat tctcaacaat ctgtcagaag caaagggcgt tttttccgca gccatcggca
     4081 tcggcttaaa accatcgccc tgctgccgtc aactattacc cttatcaacg gcatctgcgg
     4141 ttttgccgcc attggtttgg cagccaaagg cccccggcat tatgcgctgg cggcctatat
     4201 gattttttat gcgatgattg cggatatgct cgacgggcgt gtagcccgaa tcagcaaaac
     4261 aacttccagc ttcggcgggc agctggattc cctttgcgat gtcctcagct ttggcacagc
     4321 ccctgccttt ttgatgctga atctgctgct ctatcatcac cgccagctgg tcggttcagc
     4381 ccagctgatt ttaggcgatc tgtttgaacg ttttatctgg attacagcgg tcgtttactt
     4441 aagttgtgca gccattcgat tagcccgatt caatgtcgaa aacgaggaag atgaaactgc
     4501 ccatatgtct ttctcgggtt tgcctacacc cggggcggcc ggcgttgtcg cctcgatagt
     4561 aatgttcagt cagtcgctgc tgaccgatgc ggccgcccag accgttctgt atcaaatgct
     4621 ttacaacggc atcctgtata ttctcccgtt tgttacgctg gcttgtgcgc tgctgatggt
     4681 aggacggctt cgctatctcc atttgttcaa tcacctgttt cgcggccgca aaccgctgaa
     4741 ctacctgtat tttaccatct tctctgttgc tatgattgcc ttgtgcggtc tggaattgtc
     4801 gctggttctg acattcaccg gttttgctgc cagcggaccg atacgctggc tgtggcggca
     4861 gattctcctg ccgaaactgg gacacaaaac cgcagaagca gccgagcctg tccatacaac
     4921 ggcaccgtta aactgacggc ttatgagaac cacgccatct ggatgtagag ggggcggatt
     4981 gctgccgcag actgcaactg tcggacttta tgttcacata ccatattgca tcaaaaaatg
     5041 tccttactgt gctttttatt ccgaaccgat agaacaggcc gactgccggc ggttttcagc
     5101 tgcgctgctg aacgaactga ggcagcagtc aattgccgaa tcggcagaga ccatttatat
     5161 cggcggcggc tcccccagca gtctgccgcc agaggtgctt agcggacttg cgtcagaatt
     5221 gcataaacga tttcattccg tccaagagtt tacagttgaa tgcaatcctg cgcaggcaga
     5281 tgaaacactc ctgcgccgcc tgcgcagcac cggcgtcagc cggatttcca tcggggccca
     5341 gtctttccat tcacaggagc tggaagtgct cggccgaatc cacagcccaa aacagatcgc
     5401 cgatgccgtc cgagctgccc aaactgcagg atttaacaat atcggccttg acctgatttt
     5461 cggcattccg ggctccacaa ttgaaaactg gacgaaaact ctgcaggccg caatggattt
     5521 aaatgtgcag catatcgctg cgtacagttt aaccatagag ccgggaactc cttttgaaca
     5581 ggcaatacaa aaagggacgc ttgcacttat tgatgagcag accgaacgtc gaatgtatga
     5641 aaccgcacaa agtatgcttg cagaagccgg ttttgtgcag tacgagattt ccaattttgc
     5701 caagcccgga ttcgaatgcc ggcataatat ccgctactgg aaaaatctcc ctgttgtcgg
     5761 tatcggacct gccgcagcaa gctggtacaa gggcctaaga aagaccaata cggccgatgt
     5821 taaccgctac atcagcgctg tcgaagccga acaaccagca tacacccaat acgagagacc
     5881 ttctcctgaa cagacagccc gcgaaacagc tgtgctgaac ctgcggatga ttgaaggcat
     5941 caatctggct gaatttcatc ggcaaaccgg atttgatgcc atcgccctgt tcggcaagtc
     6001 tattgccgta catcttgaat ccggcctgct ggaacagaca gcaactcacc agcgtctgtc
     6061 aaaaaaaggc ttatcatacg ccgatactgt cgctgaagac attattctgt aatatcagca
     6121 cgaaaattta ttagcagtgc cccgttttga aaaatataat aatctctgtt ttactttaga
     6181 aaaagaacag gattcgctat tcttctgcta tggaactgat tgatacgcat gctcacctga
     6241 catacgacgg tctggcagac cgaattgacg atgtgctggc ccgcagcatt caggccggcg
     6301 ttgtcggatg gattgccgtg ggcacagccc aagatgaaaa caacaaggtc attgaccttg
     6361 catcccgata tgaagccctc tgggcagctg tcggcttcca tccgcattca gcggacaatg
     6421 tttcccgtgc tgatatggat cggctggcta aacagctgca gcacgaaaaa gccgttgccg
     6481 tcggcgaaac aggattggat tattattata tgcattctgc cgctgaaaat cagaagcgca
     6541 tttttcgagc gcatctggct attgccgccg ttttgcagaa accggttgtt gtccatacac
     6601 gagaggcctt tgatgataca atagaaatcc ttgatgaatt tgcagacagg ataaaaaagg
     6661 ttgtcattca ctgctatggc ggcgatgccg aacagaccaa acagatactc aaacgcggat
     6721 attatgtatc ttttacaggc acaataactt tcaagcgaaa tgatactttg cggcaaatca
     6781 tcaaagacct cgttcctttg gaccgtgtaa tgattgagac ggattgcccc tttatatctc
     6841 ctgaacccat gcgcagcgtc cgccctaatg aacctgcctt gttaattcat acagcacaat
     6901 gcttagccga cttatataac ttgccgctga cggattttgc caaccagaca accaaaaaca
     6961 gcaggctttt tttcggcttg tattgatggc ttttttaaaa acactatgga atgcacctgt
     7021 tggattgctt tcaaattggt gcagcgtcat cgataaaaaa cactttttta acaacagaaa
     7081 aaatttataa ctctatgttt ataaaatagt tataattttt ataaaaaatt cctgaagatt
     7141 ttccttgcct aagtcgaaga gaagcatata atgatagttg tagttagtgc tctttttctt
     7201 cttatagctc tatgtgtttc ttcgggtgct gaggcggatt tttgataagg ttgatataat
     7261 aaaattacaa atgcgtctca gcgaggccgg catcctcaag tgccggcctt tttattgcgc
     7321 caaccgcaat aaaagacgat tcccatccta ttattttgga agtttgcgat tgagattttt
     7381 tagattaaaa taatcgagaa aatttatacg attcaatcaa cgatttaata aatggattaa
     7441 ccgaggacag aatggacacg tgggttaggc agccggacat ggggtagatg aatatcggca
     7501 ggcattttgc gattacaata atgggcgggt tcaggatacc ttcaagcccg gcggatttta
     7561 attgggacag gattgtggac gaagggaatt tggaatagat aaaaacaatt atgaagcacg
     7621 tttgcgatgc acatttatca agacagccaa gagaatatgc cgcagccgtt ctacattaag
     7681 cccttcgagg tagtccctcg tgaaatcgag ctttatggga ccttcaaagt tcagcaaacg
     7741 gccgattact tctggcttgc tcatcgccgc aaccgcggtg catatcgtct cgaatgaact
     7801 gtctgcggtc atgtctgtgt ttccctgttc tgctgtcctg caagctcact atcggataga
     7861 tttcagcacc ccataacaac ccttataaaa aatacgaaat acttttggca ttttcaggca
     7921 tttccatatt caaacaggca atattttttt gaaaaccatc aataaaactg ctacttaata
     7981 tgtgatgaaa accgaagaat tgaattatac acttccagaa gagctgatag cccagcatcc
     8041 atcgcagcgg cgttcgcaat cccgcctgct ggttctgcat cgggccgccg gaacgctcga
     8101 agaccggcag tttgccgact tgccggagta tttgcggccc ggagattgtc tggtgcttaa
     8161 tgacacgaag gttgtgccgg cccgtttttt tattcaaaaa tcgaccggca cccgattgga
     8221 agggcttttt ctacatcagg agaaggccgg ccattggcaa atcctgctga aaaacgccag
     8281 acgcataaaa tccggccaaa cagtcgatct gctggaccgt gccggcaaat catggtgcaa
     8341 agcggttata agagatggag atgttccaga acagagatgt cttgtattgg ataagccgca
     8401 ggatgctttt tttgttcttg acaatatcgg acttcctccc ctgccgcctt atattaagcg
     8461 ttccagcagc ataccagaag acttgaaccg ttatcaaacc gtttatgccc gccgtctggg
     8521 tgcggtggcc gccccaacgg cgggattgca ttttgaccct ccccttctgg aaaagatcgg
     8581 tcagaatgga atccgcatcg caatggttac gctgcacgtg ggtatgggaa catttcgtcc
     8641 tatccagact gaaactctgg aagagcatcc aatgcatgaa gaatggttcg aaattagcga
     8701 cactgccgcc gatgttatca atcagacaat ccagtcaggg gggcggataa tcgccgtcgg
     8761 gacaacttcc gtccggacac tcgaaagcac ggcggatggc cgccgcatcc gttctgctca
     8821 ggggcacacg cggctgttta ttcggcccgg ctatacattc aaaatcgtgg atgccatgat
     8881 caccaatttc cacttgccga aatctacatt gctggcactg gtgggggctt ttgccggcct
     8941 ggacagaatt ctctatgcct atcagcatgc tatcaagcaa caataccgct tttattccta
     9001 cggcgatgcc atgctgattt gctgaattca gacgactatc tatgcagtga tagtctatct
     9061 ttacaaaact atctgaccat aattcctatt cctgcagcgg ctgttatctt ctggaagcca
     9121 gtgaacggcg taccgatttt tttgttttct gattatccgt ctctgcatgt ccttccagaa
     9181 ctttcatgga tttttttaca tccgccacct tcagaactgc ggttgtgcgt ccgcctctgg
     9241 caccggctgt gcagtacgca taagagatat tgatatgagc tttggctaat ttgttggtca
     9301 catcggcaaa ggctccggca tgattgggca gattgacgca taaaacctgt gtttcgctga
     9361 attgcatgtt cattcgctgg agaatggagc gtatcttgtc cgatccgctg ccgaccagcc
     9421 gcagtacccc atgctcaaca gaatccatca ttgtcattgc aacaatgttg attttttctg
     9481 ccgccagtac attcagcaat tgagccagca caccgggctt gttaaccata aagattgaga
     9541 attgcgtgtc taaatgcatg gcattatcct ttcacataga atcccaggtt tgccttcgtc
     9601 cttaaaaacc gaatccgttc gtcagaccga tttattggat tccctgcagc tgcagtgcag
     9661 ctgccgctgc ggcggaattt tcataaaatt caaagagctt atccagatgg gtaatacgga
     9721 atacacgtat cagctgagga ttgatcccgc agataagaag tttgccgccg gcctctttca
     9781 tcctccgcca caagtccacc agcagcccca gcatttgtga tgaaaaaaaa cgcacattgg
     9841 aaaaatcaat tatcattttg gcgggatgct gcttgcagac aagagcacga aggatatcgg
     9901 caattttctc tatttccccc atgctcccaa tggatgactg ccggaacaaa acaatccata
     9961 tatcctcacg ggtcgtaatg tccagcaatt cgttgtcatt tctctgattc aagaaatact
    10021 ccgtctcacc catcgctgtt tctatcaata gtataccaat ctttccgctg tttgtcaaac
    10081 attaaacagc acttcgcatc tggccaatcc tgtccggcga tgtcttccag caggctgccc
    10141 aatgctgacc ttgctggtct ttggcttcca gctgctgggt ttcttctgca caccgttttt
    10201 cggccatcgg gcagcggggg tgaaaaggac agcccgatgg gggattggag gggctgggca
    10261 cctcgccttt gaggacaatg cgttttttct gccggcttgg atctggctcg gggatagcac
    10321 tgagcagcga taccgtgtaa ggatggaggg gatctgcata gagtgtatcc cgattagcat
    10381 actcgacaat atgccccaga tacattactg cgacccaatc gctgatatgc tcgaccacag
    10441 ccaaatcatg agcaataaac agatatgtca gattcagccg gctctgcaaa tcctgcagca
    10501 ggttgataat ctgcgactga atagatacat ccagcgcgct gaccggttca tcacagataa
    10561 gaaacttcgg attcagcgcc aatgcccgtg ccacgccgat gcgctgccgc tgaccgccgg
    10621 aaaactcatg ggggtagcgg ttgagatgct gtgctgacag ccccacctgt tccatcagtt
    10681 ccacaatccg ctgacgccgc tgcttggcgg acatagagcg atgaacgatc agcggttcgc
    10741 cgattatatt ttctaccgtc atccgcggat ttaaggaacc aaacggatcc tgaaacatca
    10801 tctggacatt ctgccggaag tttttcatct ctccagccga cagggaaaag acatccattt
    10861 ggtcaaagat aaaagtccct tgtgtcggcg gaatcagccg catcagtgtc cgccctaccg
    10921 tggtcttgcc gcagccgctt tcgcccacca gcccgagtgt ctgcccttgc cgaattgaaa
    10981 aactgacacc atcgacggcc ttcacccagc cgacagtgcg gctgaagatg ccggcccgaa
    11041 taggaaacca agtctttaaa tccttgacct caaccaatgt tttggatggt tctttcatca
    11101 cttccgtttt cagcgttaac agctgaattc ctatgttgaa tttctgccct tgaaactgct
    11161 gtcataccct tctgcatacc agcaggccgc acagcggccg ggggatacct cccgcagggg
    11221 cggttctgct gtccggcagc gggtatcctt gcagccgaca ggacagcgcg gatgaaactt
    11281 gcagccggct ggaaaatgga gggggttggg aacagcgcct ccgataacat ccagacggtg
    11341 ctttctggca cccagccgcg gcagcgagcg cagaaggccc tgtgtataag gatgaagcgg
    11401 cttttcgaac aactcttttg cacacgctgt ttccacaacc cgcgatgcat acatcacaac
    11461 gatatcgtct gcattctcgg caaccactcc aagatcatgc gtaatgagca taatgctcat
    11521 gccgctttcc tgacgcatcc ggcggagcaa atccagaatc tgtgcctgaa tggtaacatc
    11581 caaggcagtc gtcggctcat cggcaatcag cagactgggc cagcagcaca gtgccattgc
    11641 aatcatgacc cgctgacgca tccctccgct catctgatgg ggatactcgc ggacccgccg
    11701 ctgcggatct gcaatgccga ccttgcgcat catatccacc gcaatatccc acgcttcaga
    11761 accgctcttt ttctgatgca gccgcacagc ctccacaatc tggttgccga cagtaaaaac
    11821 cggattcaag ctggtcatcg gctcctgaaa aatcatcgca atatcgttgc cccgaatctg
    11881 ccgcatccgg ctttcggccg catcgagaag cgattcaccc ttgaacacaa tccgcccttc
    11941 gacaatccgg ccgggcggcc aaggaatcag acgcataatc gacagcgctg taacactctt
    12001 gccgcagccg ctttcgccga ctaccgccag cgttttgccg gcaagaatat caaagctgac
    12061 atcctgcacc gccttgacca gcccctgctc ggtatgaaaa tgagtcgtca ggttccgcac
    12121 cgacagcaaa atgttattgt tttcactcat tcccggtttt ttcagcagct gctgccgtct
    12181 ccagcagcat tgcagaccct gctgagcatg ttcacccttt ctttcagtct gttctcagtt
    12241 tgggatcaag gcagtcccgc aaagcatcgc cgaaaatatt cagtgccagc gaaagaacaa
    12301 aaattgcagc tgttgcggcg gccatttccc accacacatt gcgcgccagt tccgttctgg
    12361 ccgcatcaat catcgccccc cagctgggct tatccgtttc gcccaatccc agaaaactca
    12421 aaatcacttc tgcgtgaata aaactgacaa accgcaggga aaaatcaatg ataatcacat
    12481 gaaagacatt cggcagcaga tgcttgaaga taatccgccc gttggagcag cccagcgccc
    12541 gggccgccag cacatactcg ctttccttgc gtttgataac ctcgccgcgg atcagccggc
    12601 agatacccac ccagctggtc aggcccaagg ccacataaac ggctgtcacc cctttcagtt
    12661 caactccaaa gaatgtctgg ctgcgcagca caaccgcaaa ggccataatc aaaataatcc
    12721 ccggaatcgt accgatggtg gtataaagcc agacaatcaa atcatctatc caccccctga
    12781 aataaccggc agcagcacca aggggaacgc caatcaggat gctgataaca gaggcaaaca
    12841 gggcaacggt gatgctggtc ttggcgccgt agattgtctt gcgccacgta gattgcccga
    12901 ggaaatcggt cccgaaaaag gtccagccgc gctgggcttg ggtttgccct tcgggcatcc
    12961 acgcatatac cgtccatggc ttttgatacg acggcccaac tgcttgacgc cagtcaaaca
    13021 agggtatttt ccagccggcc gcctcgctca gatacaccag aacagtcagc agaacataca
    13081 ccatcactac gccgaaacag accattgcca aacgccgctt gcgcagacgc cgcatgccgt
    13141 cggcccacag gcttcggcct ttaaccggct ggtcggcaga tacggctgtt ggctgactgt
    13201 tattcaaatt taatcctcgg atcaacccat gcatagcaga gatcggccac cagcgtaaaa
    13261 aaggcaatca gcagggccat aataaacgtc atcgcattca gtacaaaaaa atctcgtgaa
    13321 gaaaccgcct catacatcag atagcccaag cccggaatcc cgaaaaaccg ctccagcagc
    13381 aatgagccca aaatcagata aggaatcgat aaaacaacgt tggttacaat cggtatcatt
    13441 gcgtttttca agacatgctt gaacagaacc cggcgggcgg tcagcccttt ggcaaatgct
    13501 gtgcggacat agtcgctgcg gatttcatcc agcataatcg tccggtaaaa ccgcaggttt
    13561 gaacccaagc cggcagcaat gcctatcaga accggcagaa caatgctgct gaccatcggc
    13621 cggtccgggg cgtaaaatat cgggaaccag tccagcttgt aggcaaagaa atactggccg
    13681 aagatgataa agctcagata cggcagactc atccccgcca cacacagaat cacaataata
    13741 tgatcccaaa ctgtcccccg cagaaaagcg acaaccagtg caaaactgat ggaaataacc
    13801 agagtcccga aaaacatcgg cacggtcagc gccagcgagg gccccacccc gcggagaatt
    13861 ttttcgctga tgcgctcccg gtccagtgcc cggccgaaat gaaaggtaaa ggccttcttg
    13921 aaatgattca gaaactggct gtcccagctc aaaaacaggg gtttatcaaa accgtattcg
    13981 tggcgaatct cggcgatggt ttcggcatcg gcattcttgc cggcaatctg ctgcgaaata
    14041 tcgccgccga caagcgtaaa gagcacaaat gtcagcagca gaaccccgat aacaatcgga
    14101 atcatataca acaggcgtcg gatgatatag gccgtcatag ctttttcttt tccagttccc
    14161 tgacccgcct cttataattc ttttgttcag ccgtgtcaat ccgaatatac ttcatcgtgt
    14221 cataaatata cggatggggc ttgtagttct tgatccatcc gtgatacagg gtatactgca
    14281 cgcggtgatt ggtaaaaatc gccggacagt cttccagcac gattcgttcc atctttcggt
    14341 acagctgggt ccgttccgca cagggaaaca taacttccgc ctgattgtat aatcggtcaa
    14401 attccggatt tgaataaaaa aagaaattcc ccagcggcgc aaagtacttg gtagcaaaca
    14461 tcatcagcac attcagcgca tccggggtac tgaaccgcac tcccccgccg ccgaatatct
    14521 ggtgttggcc tttgttcatc ttttcgacat aggtcggcca gtcggtaaag tccagccgca
    14581 ccttaagccc cagcgattca aactgccgct gcacaaactg gccgaactga cgcgaaaacg
    14641 gatccccgcc ggtcatccca atggtcagct cgggaatagg tcctccctga atccgctgag
    14701 cttcggccag aagccggcgg gctttctgca tatcaaaacc catatacggc tcattgacaa
    14761 cattcggatc atactcattc agcaaaggat gaataatgct gtgagccacc cgccatcggc
    14821 cgttataaaa cagatcgttg agcttttcac tgtcaaacgc atagttcagc gcctgccgaa
    14881 gcggcttatt ttgtcccaac accgcatcct gcatattgaa accaatataa tacagcgacg
    14941 gctggtctga aatggccagc tgaatcttcc gctgctccag aaagtccttc ggccgctggg
    15001 cctgcatatc gaccgctgtt gcaaaattat ctttcggtat ccccatcatt tccatatccc
    15061 cgcgcagaaa caaaagccag gccggctgct cttcctcaat aatccgaaag acaatgcggt
    15121 caataaacgg cagcgtcttg ccggcatcgg ccagcaggcc ggcggccgca tctgccggct
    15181 cgccgtccga aggataacag tccccgcgaa aggccggatt tctctccagt tccagaaaaa
    15241 ctccccgcgc ccacttcttc agccgatacg gccctgtccc gaccggatgc accataatat
    15301 ccttgcggta gtaatctacc gcttctttgg caacaggagc tgtcgcggca ctggtcaaat
    15361 catccagaag ctggggccac ggtttggtca ggcgaatctg cagggtataa tcatcgatcg
    15421 cctgcaggcc ttcgacttct ttgctgtaat caacctcaaa ctcgtcctta aactgcttgg
    15481 tgtattcgcg gaagtcatcc aaaccggcaa tccgctcctt aaagctgccc cagttcacac
    15541 tggcatagcg gacattggca atccgcttaa acgcaaaaac aaagtcttct gctttcagct
    15601 gacggccttt ccgatcggga aagcacggat cgtcctgaaa ataaacgccc tgcttaatcc
    15661 ggattgtata ggtcagctta tcaggactga taacaggcaa ggcttcggcc agcagcggaa
    15721 taacttcaaa aggacgcttc agataatggt aggtatacag cggctcgcaa atatgggaaa
    15781 ccaccaagtt gctgtataca tcatcgatat tgccggggtc caaactttta accttgacgg
    15841 gcaaaggata gcggaatacc gcttcatctg attcactgcc tttttcagaa cagcccgccg
    15901 gcagcaggca taaagccgca agaacaacaa aacctgccga tttaaccttc cacttaatca
    15961 tattaatcat aaaaaagtcc agtctaaaag ccgctgtccc aataccgcgg cctgaaatca
    16021 cctgaacatc tttcgatacc tatcccgctg ctcggcattg attttgtaat accgcagctg
    16081 tccgaaaccg attgtgtcgg ctttataggg atccgcttta acattgccaa gccagccgtg
    16141 atgaatgata tatccaatcc gatggtaaat aaacgcacac ggcatttgtt ccaccacaat
    16201 ccgttcggct tttcggtaca gctccgtccg ctccgggctg tcgggcataa tacagacttt
    16261 ctcgtaaatc gcatcgaatt cagcattttg aaaaagactg ttgttcggcc acggggcatt
    16321 ggggctgtaa aagactccca gaaaactttc cacatcggga taatcggcca tccagccggt
    16381 aaaataaagc tgcaggtcgg aagtccgcag tttttcaaga aacgccggcc agtcgtaaat
    16441 ctcaatttct gtctggatgc caatctgcat cagacagcgg ctcaaaaact gcagcgtctg
    16501 cttctggacc gtgccggtcc gtccaatcga cagccgcagc ttcgggatag gaccgccctg
    16561 tatcttttcg gcctctttca tatacgctct ggctttttcc aaatcaaacc ggcaaggcga
    16621 ccagtcggca atcgactgat catagccgtt catcaccaca ggaataaatc cgtaggccgg
    16681 cttgcctttg ccgctctgga tgatttcaat aaacttcagc cggtcaatcg catagtttat
    16741 tgcataccgc aggggtttat ttcggccgat gacgggatcc tgcatattca ttccaagcca
    16801 gaaggtgcag ggctcatcaa acagctgcag cttcatcccc cgctgcttca tctggtctgt
    16861 caggtcagta ctcatcgata cggcttgtcc aaagttgtcc ttgggtatcg agttgatatc
    16921 aatatccccc cgcatcagca gcagccagcg cggctggtct tcggtaacaa tccgccagat
    16981 aacccgatcg ataaacggca acggcttgcc ggcatcttcc aataatcccg cttcggcatc
    17041 accgtctgcc ccttcggacg ggtaataaac cctgcggtaa tggggattgc gtacggcctc
    17101 cacataaacc ccgcgatgcc actgtttcag gataaacggc ccggtgccga cagggtgatg
    17161 aacaatatcc ggcccgtagt agtccaccgc ctcttgagcc attggcgctg tcggcagaaa
    17221 tgccagccaa taaatcagct gcggccacgg gcggatgagc ttgatgacca gcgtataatc
    17281 gtcttgggca tacagccctt ccactgtgcg gctgtaatcc acctgccctt tttggcaggt
    17341 cttcgtatat tcccggaatg catccagccc cacgattcgc ccatcaaaga tgtaccagtt
    17401 cttgctctgc accttcacat ccgccagccg ctttatggca tagacaaaat cctgcgcttt
    17461 cagttctcgg cctttgccgt ccggaaagca cgggtcatcg tgaaaataga tgcccctctt
    17521 tatccgaatg tgatattcag tgccgtcttg gctgatttcc ggcagggatt ccgctaattc
    17581 gggtacaacc acatgaggac gtttgaggta atgatagccg taaagtgatt catagaactc
    17641 tcgacacact ccatctgaat tggtatcgcc tacatccgcc ggatcaagcg tctggatgtt
    17701 ggcctccagc cgctggttga gaacaacctc cccctgcccg gccgagggct gtttggattc
    17761 acagccgctg ccgatgaacg caatcattac tgctgcgatg ataacgcgtc gacaaatcaa
    17821 gtctgccttt ccatgctaca aaacattaaa actgatccag ccgcatgaac agccccctgt
    17881 acagaaaaaa tgcggatctc tttcctgcgg catcaccgct ttggagcttg tccgcttttt
    17941 caggatttac tcgcaagcac gacaggacat gaatccagtg gtgctcattc aggagacttt
    18001 gcggcttcgg gcatcgggtc cagttccgcc ggaaatgtaa tcggcttgcc gga
//
LOCUS       DAOTBO010000056        18035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2577_length_18035_cov_5.105840, whole genome shotgun sequence.
ACCESSION   DAOTBO010000056 DAOTBO010000000
VERSION     DAOTBO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 18035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18035
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2577_length_18035_cov_5.105840"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLE88_07715"
     CDS             <1..193
                     /locus_tag="PLE88_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76388.1"
                     /translation="GDVRQQAQQWIQNSQITSKDGQNPLTVAYDSQRNTTVVKAAAAV
                     DSLPMRQDQMQTLPAGSLK"
     gene            237..389
                     /locus_tag="PLE88_07720"
     CDS             237..389
                     /locus_tag="PLE88_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76389.1"
                     /translation="MRCTIGLEESRTLYPTGYSINSGDGMRQLHAAGGWEIQKGVAVM
                     RDWLSG"
     gene            467..2095
                     /locus_tag="PLE88_07725"
     CDS             467..2095
                     /locus_tag="PLE88_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76390.1"
                     /translation="MMKKILSFMLLGMLFAAPALALIDDFDAVHNYKTDGVAGTGWDN
                     LFAVGGVQSSLQVQSGSGKLRLQSQGTTYNAPWDMNNDGIRDVGPSLTVSQTGDFVMI
                     TRFLGESGTSASPVFHQSAGLIARNPNTASGENVVLSAFFPVWTGHITWNVVNDVRTG
                     ESGSTVAGWSDRPGAFAAAARYCFMKLERTGNQFRMYISQDGVNWLPLVIQGPAIYNG
                     TQTPVVWTRNDMPETIQIGLFHAIYEQNLGWSDFDFVYAGRQVAAITQNPVVREEGQT
                     STDLTITLTGPAPSAEVQVKVIPYAIPYTYTAEDNDPNDILLVGSEGQGLPLTVTFPV
                     GTTQQTITVKATEDALAEGLERIGLKVLTYSTDPAYDQQLGQWTLVTVIDNEQGGLEI
                     GQGDGLVVDEDGLLTDTFTVSLTQPPANDVTVSITTDGQTTVNPASLTFTTANYSTPK
                     TVSVKGVDDAVLENDPHVSILKFSISSEDAAYNGVSVPDISVQVRENECGAWGYSPYD
                     ENEDCVVNLTDLSALAAHWMECTLPNAAGCLDAR"
     gene            2160..4772
                     /locus_tag="PLE88_07730"
     CDS             2160..4772
                     /locus_tag="PLE88_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76391.1"
                     /translation="MKKLVQLICLFALLTIAGAASAATLLIDLRADDLPEGDLRSWTN
                     QGAAGGVFTIADRPDDGIVDPNAVVQTVAGRKAVTFAGGAWMQSDITAPAGLTGAHPW
                     TVMVWAYATSTTGEDCLFQWGDRGGGTAHFNFGTRAITFWNDVFYTNAPALNQWVHLA
                     ATYDGTNVRLYVNGTDDTVAARSLNINTGNYMRIARAMPNLWGTHPFGGAIASIQAYD
                     AALTKQEIQVLTGLFEAGSLSPTGVSVRENGPASTIYVQINTNPITGQGPQTDLEVTL
                     SLISTQKDIQLGTSPVGQPYVITVPAASYQTPIPISVLAEDDDIIESQHAVRVRAVVT
                     AGDPDYLGKILVPNNGLAITVIDNDYTPCTAPGLTNGAYVDHFDCKWDYTQGPSSMWS
                     GLMDTAGYTTDATSSISAAGALRLASANAFWSGNDTTGPYLYIDVTGDFEVETYISEY
                     ANSIAGQDGVLHNAGGIMVRLADPAAGGAGEDNIQASYFPIWGCGNILWYHNNGARTE
                     TDIADTAWDGSRYLKVVRKGAAFYCYHSPDGNTWIPFPSANPMIRQDMNVPTLQVGLY
                     QCTFNNTLGFVDYDYFSLKKVRGHLTGTLNLTECDADAGTVQFQFETDGVKAPIADIN
                     VTVYAVAASGADPNSDPNDVVIGTAEMGQPYTFTVSPAQYSQPQLIQVAAVADATPEP
                     DQLLTLQAQIASTDPNWNGLLISSDVLIHVMETPGLLLDTGDGVRVAEGDGPDTFTVR
                     LKIPPLAWPVSVNITNAAGQVTVDPAQLSFDAASWKTPQVVTVSAVDDAVLEADPHAT
                     TLTLTAANGNEYDALAPLTVSVSILENECGAWGYVWGDFNRDCKVDVADLSEMAAAWL
                     DCTNPYDTGCVDLR"
     gene            5069..7459
                     /locus_tag="PLE88_07735"
     CDS             5069..7459
                     /locus_tag="PLE88_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM76392.1"
                     /translation="MFKDAGEMGVSIVFAAAVFFQAIVGAQMNETIGYQKEQLLPARP
                     LELSKVRLLGGPLKQAQDKDAEYLLNLEPDRMLAYYRRQAGLEGKAAPYGGWDGGGRN
                     LTGHIAGHYLSAVSLMWAATGDQRFKDRADYIVKELKEVQDKHGDGYLSALEGGRECF
                     EAVRRGQIRSGGFDLNGLWAPWYVLHKTYAGLRDAYRYAGSKTALEMEIKFAAWAEGI
                     LADLTDQQVQKMLNTEFGGMEEVLADLYADTGDIRWLKLSRRFEHHSITEPLKRHQDI
                     LSGKHGNTQIPKLIGSADRFVYDGQAGDLLAAAFFWDAVVQHHSYATGGHGKDEYFGD
                     PDRLSSRIDGRTAETCNVYNMLKLSRRLFSLRPDTHYAEFHERALFNHILASIDPEDG
                     RTCYMVPVGQGVSHEYQDMFGSFTCCVGTGMESHALHADGIYYESGNKLWVNLYAPST
                     ADWSGITIKMETDFPEGDSAALTITAEQPRPFVLALRRPAWAGEGFAVAVNGSPVDNL
                     AQPASYVELNRTWKSGDRIELTLPKRLHLEATPDNPRRVAILWGPLVLAGDLGPEQRR
                     QRSAPREESERIPVFVAADQPVEQWLKPVEGQPGHFRTAGVGRERDVDFVPFYKLHRR
                     TYGIYWDLFTESEWQAKAAEYAAAQERRRKLEQATVAYAQPGEMQPERDFNYQGADDV
                     RVERLGGRPCRAGGSWFSFDMPVEPEHPMTLIATYYTGQWLRQPAVFTILADGRKIGT
                     ETVVRRDPYEFYDVEYLIPPDLVKDKKKITIRFEAAPESRIAAVFGLRMIRADASR"
     gene            7504..10185
                     /locus_tag="PLE88_07740"
     CDS             7504..10185
                     /locus_tag="PLE88_07740"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM76393.1"
                     /translation="MKAMQLLFSAGLLGMIGYCMAAEESARPEINLAVVAVPSSSYVS
                     GDTSLEALNDEAQPRSSRDRRQGAYGNWNRTGTQWVQYDWSQPISTRKIDVYWWDDRQ
                     GVRLPKACRLFYWDGQKFVPVSNPVGLGIAENQFNTTTFDEVQTTKLRLEIDSDGSYS
                     TGILEWKVYDSGRSPAFAPRVSAGVDRVVVLGGRTYLKGSVKTLDGRTETLRWQKSEG
                     PGDVVFEDASKSDTAAVFSKAGEYVVTLTAGKEPLTASSSLRVKVIPAPPKEPLRAVD
                     THRYTIQSPLWNSRAKALIVNWIPHCIRKISDPQLREGGINNFIEAAKKLAGQEHGQH
                     KGYVFSNAWVYNTIESICIALQIDPQGDADIIAAQKMMQETLEDWIPKILAAQEPDGY
                     LQTAFTLSNRQRWSPRFRGDHEGYVAGYFIDCAVAHYEMTGRKDTRLYDAAIKLADCW
                     YNAIGPAPKQEWYDGHQAMEMALVRLGRCVNDAQGQNKGSKYIQLAKFLLDCRRDGSE
                     YDQSHLPVIQQYEAVGHAVRAVYSYTGMADVALETGDIDYQSAVFSLWDNIVNRKYYV
                     TGGIGSGETSEGFGPNYSLRHGGYCESCSSCGQIFFQHRLNLMTHNALYADLYEETLY
                     NALLGSVDLEGRNFYYQNPLISQGRRYDWHACPCCVGNIPRTLLMLPTWMYAKGPDAV
                     YVNLFVGSTVKLEGIAGTDVQMVQKTDYPWKGTVEITVNPVSQAEFTVFVRAPHRSVS
                     ELYSDIPPADGIASLSVNGQKINPAVENGYAVIKRKWKAGDRIELVLPMQIQRIKGID
                     KIEATRGQAALRYGPVVYCCERIDQPLDKVLPPDAALETEWRADFLQGVMVIKGSWYD
                     GTPLLAIPYYARDNRFPDEQSRPRDRGPASSVWLKER"
     gene            complement(10263..10577)
                     /gene="rhaM"
                     /locus_tag="PLE88_07745"
     CDS             complement(10263..10577)
                     /gene="rhaM"
                     /locus_tag="PLE88_07745"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:TIGR02625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOM76394.1"
                     /translation="MIRKAFRMQVYPEYQDEYQRRHSPIWKQLHQTLKDHGVHNYSIF
                     LDKETSQLFGYVEIEDPKRWDAIAQTGICQKWWAYMKDIMPSNPDNSPQSRELTEVFH
                     ID"
     gene            10727..11398
                     /locus_tag="PLE88_07750"
     CDS             10727..11398
                     /locus_tag="PLE88_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76395.1"
                     /translation="MKTTIKLFTGLAGIVLVGAGGLFLWHQVSSKRTIEQLLRENETL
                     KTAISNLTQESQIGYAKVLSQEIRDGAVYTKLLFVEVDPSDFTKHLLRREYEIEGDVV
                     HFDTLIVTFGSQLVMDGKERAMYLWRRIYGEKMPPEQGFPIEQEGQPSPRYSELCAKL
                     SIEDRKLFWDEIWSLSNNPKRLEKLGIKAVFGNAVYRQLKPGLIYVFKISPTGSLYPE
                     IVPDL"
     gene            11467..13806
                     /locus_tag="PLE88_07755"
     CDS             11467..13806
                     /locus_tag="PLE88_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOM76396.1"
                     /translation="MKIQSVKGLICLLSTIGLSCAAFSQQDGCPEGKLAPKPLYRDPV
                     YDGAADPALCWNRAEQKWFMLYTNRRANIQNAEGVSWVHGTPIGIAESSDGGATWTYR
                     CDANIGYQKGSDTYWAPEVIEHKGLYHMYLTYVPGIFSDWNHPRDIIHLTSKNLIDWE
                     YQSTLKLSSDRVIDACVAPLPEGGWRMWYNNEKDRKSIYYADSPDLYVWEDKGKAVGD
                     RAGEGPTVFDWKGHRWMIVDNWQGLGVYRLQEDGSWLRQSQNLLQTPGRGNEDGVIGQ
                     HPDVIVSDERAYLFYFTHPGRTGENARRDAYEQRRSSIQVVELDYRDGWLTCDRDKPT
                     CIMLKAPALEFNVCDYGAVGDGQTVCTQAIQKAIDACAKAGGGTVVLSVPGTYMTGSI
                     FLKDNVNLRLDRAVVLKGVQDESAYPEVYTRVAGIETNWPAALVNAHDLSNVKIYGQG
                     TIDGSGSMWWDKYWNMRREYDAKGLRWVVDYDCKRPRLILIYNASNVSLKDLTLQEPG
                     FWTVHICYSSHITVDGLIIRANLDERVGPSSDGIDIDSSNNVLVQNCDIDCNDDNFCL
                     KAGRDADGLRVNRPTHDVVIRDCIARRGHGLITFGSETSGGIYNVEVYNLKGYGTSTG
                     IRFKSTQGRGGTVKDAYIHDIQMVNVRDAIMLDYDWYPAYNTVPDAVRRQIEAEGKEL
                     PRHWKALMEQVPEDKGTPYIRDIRIANIHAVDSQTAITISGRRNQRAGNFLLENVRIT
                     ASKAGTIKNAENWTFKNVVIEAKDGSTVKFENCENMTGL"
     gene            complement(13971..14717)
                     /locus_tag="PLE88_07760"
     CDS             complement(13971..14717)
                     /locus_tag="PLE88_07760"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOM76397.1"
                     /translation="MKKVIVLLLTIWLGTGVEALLITTADGNGADGWISNDNQSSSYW
                     SNTVHNTTGMQARYNGGGSRFRATFMRFDISSVSGPIAADAKLQLGQTFTKGSDKVMH
                     VYGLVDGDAGELWSETTLCYDNAPGFLTPPEGNNLGYYAIDPARLILLGTFIIPGLGS
                     SSGTTLTPPVTILTHPATLPLADFLNADTNGLVTFVLINAAGTSSNNSENRFASKEDA
                     NGGWPTLVIVPEPATLVLLGLGGLLLSRKR"
     gene            complement(14808..16118)
                     /locus_tag="PLE88_07765"
     CDS             complement(14808..16118)
                     /locus_tag="PLE88_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76398.1"
                     /translation="MLSWTNPEPNNPGETITCDIYFGTTEPNALEPHWGLPLLQAGVI
                     GNSVAIPPLAQFTTYYWVVDIHDSSFPDRTVPGFVWVFDTNNAAPVVNAGPDQYVWLT
                     KAVVNTTSDADTYLRDTTVRGAEPVIDIRGGGTDFAGYLRFDLSALTAMGPGVISNVT
                     LTLTISGGASRNDTPTNARFALYGLNNVPGNTPQNWSEAVLMDTNTGQEWNGTMPMTT
                     ALANGWITDLDDNVEGIVETIGGSAVGSKITISGPPLEAFLKSRIDDNGLVTFILAND
                     DGTDRGFGVGSKENSNPDYRPNLTLTYVPDSAANNGDAAVTLSGTVTDDGLPDPPAAC
                     TVQWTQVSGPSTVTIDPADTLETTVYLPLLGTYVFRLTADDSNMTASDTVQVYVGTDP
                     CDAAQNTPGYSANAADFNRDCMVNLKDFAAFAAQWLDCNSYVCP"
     gene            complement(16112..16837)
                     /locus_tag="PLE88_07770"
     CDS             complement(16112..16837)
                     /locus_tag="PLE88_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76399.1"
                     /translation="MRNLLFSIVLLSLLGLVAHAELAVTTAAGNGADTYVANDDGSQS
                     PDGNFGTEPRMRIRVNGTNRVKIAYIRFDLSNVLLDPTDAYLKFETTYMKSGGKSVNV
                     YGLTDDRLDYWIESGTGGITFNTAPGQVLPAAPGSYTLDTAKTKLLGTFQTSTTAAPV
                     ILTSNPTNLDLASFLRSDTNGLVTLLLIGQDDETELATKENTTAGITFPTLVLPDSNE
                     ILLPVQQHRFRLPMQRFRQASIC"
     gene            complement(17234..>18035)
                     /locus_tag="PLE88_07775"
     CDS             complement(17234..>18035)
                     /locus_tag="PLE88_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM76400.1"
                     /translation="QNSGGNSEPSAPGYLAVKRLLAQWGFNGDAVDQTGVYNGTIYGE
                     PNFVIDGNHHAMEFYGDNDYVDLPDGFENFRAGLTFTVWAKPAAVTSWARFIDLGNGP
                     NSDNIFLTRNGTTSNLTFNCYEGNASAGAVTANNALVLNEWQMFAATVDEAGNVVLYK
                     DGFSIQTGSISKKINYVTRTGNFIGESNWEADALYNGLIDEIRIYNYPLDADTLATIY
                     SDVAGPYCRFKPVLDYSGNCVVDLEDFTVYASSWLECGLWPVTACDEL"
BASE COUNT         4299 a         4421 c         5033 g         4282 t
ORIGIN      
        1 cggcgatgtt cgccagcagg cccaacagtg gattcaaaac agtcagataa caagcaaaga
       61 cgggcagaat ccgttgacgg ttgcgtacga ttcgcagcgg aatacgacgg tcgtaaaagc
      121 ggccgcagct gtcgattccc taccgatgcg ccaagaccag atgcagacgc tgccggccgg
      181 cagcctaaaa tgaaacgagt acttgcggag gactggcatg tgaaaagata cgggtgatga
      241 gatgcacaat cggattagaa gaaagcagga ctctgtatcc gaccggatac agcataaaca
      301 gcggggatgg gatgcggcag cttcacgctg ccggcggatg ggaaatccag aaaggggtgg
      361 cggttatgag ggattggttg agcggataga aaaacaggag ttgtattgca cagtgtgccc
      421 ttgacgggct gaaatccaac atatcaaatt gtttttagga gtgctgatga tgaaaaaaat
      481 actgtcattt atgctgcttg gaatgctctt tgctgcaccg gcactggccc ttatcgatga
      541 ttttgatgct gttcataatt acaaaaccga cggcgtagcg ggtacgggct gggataatct
      601 tttcgccgta ggcggcgtcc agagcagttt gcaggttcaa agcggttccg gcaagctgcg
      661 tctccagtcg caagggacga cctacaatgc tccctgggat atgaacaacg acggcatccg
      721 cgatgtcggg ccttcgctga ccgtcagcca gacaggggat tttgtgatga ttacccgctt
      781 tctgggggag tccggcacct ctgcatcgcc ggtatttcac cagagtgcag gtctgattgc
      841 acgcaatccg aacaccgcat cgggtgaaaa tgtcgtgctc agcgctttct tccccgtttg
      901 gacaggacat atcacctgga atgtggtcaa tgatgtccgc acgggggaaa gcggctcaac
      961 cgtggccggc tggtccgatc ggcccggggc ttttgctgct gctgcccgct actgctttat
     1021 gaagctggag cggacaggca atcaattccg tatgtatatc agccaagacg gcgtcaactg
     1081 gctgccgctg gtaatacagg gaccggccat ctataacggc acgcagacgc ccgttgtctg
     1141 gacccgcaac gatatgcccg aaactatcca gattggtctg tttcatgcga tttatgaaca
     1201 gaatctcggc tggagcgatt ttgactttgt gtatgcaggc cgtcaggtgg ctgcgattac
     1261 ccagaatccg gttgtccgtg aagaggggca gaccagtacc gatctgacca tcacgctgac
     1321 cggtccggca ccgagtgcag aggtgcaggt caaagtgatt ccttatgcga ttccttatac
     1381 ttatacagcc gaagataatg atcccaatga tattctgctg gtcggctcag aaggccaggg
     1441 actgccgctg acggtaacat tccctgttgg aaccacccag cagaccatta cagtgaaggc
     1501 tacagaagat gctttggccg aaggtttgga acgcatcggt ttgaaagtgc tcacctacag
     1561 cacagacccg gcctatgacc agcagttggg ccagtggaca ctggtaaccg ttatcgataa
     1621 tgagcagggc gggcttgaaa tcgggcaagg cgacggattg gttgtggatg aagacgggct
     1681 gcttaccgat accttcaccg tctcactgac gcagccgccg gccaatgatg taacagtgtc
     1741 gattacgacg gatggccaga caacagtcaa tccggcttcg ctgaccttta ccacagccaa
     1801 ctacagcaca cccaagaccg tcagcgtcaa gggagtggat gatgctgtgc tggaaaatga
     1861 tccccatgtc agcatcctca aattcagcat ttccagcgaa gatgctgcct ataacggcgt
     1921 ttctgtaccg gatatctctg tgcaggttcg cgaaaatgaa tgcggcgcat ggggctattc
     1981 tccctatgat gaaaatgagg actgcgttgt gaacctgacc gatttgagcg cattggccgc
     2041 ccactggatg gagtgtacat tgccgaatgc tgcgggctgt ctggatgcac gctgaggcag
     2101 gcaatcaaga cacaggcgtg taaaattgtg gaaagaactt tattttggga gatatgaata
     2161 tgaaaaagct tgtacaactg atatgtttat tcgccttgct gacgatagca ggtgcggctt
     2221 cggctgcaac gctgctgatt gacctgcgtg ctgatgatct gccggaaggc gatttgagaa
     2281 gctggaccaa tcagggggca gccggaggag tatttaccat tgccgacaga cccgatgacg
     2341 ggattgtgga ccccaatgcc gttgtccaga cggttgcagg ccgcaaagct gtgacctttg
     2401 ccggcggtgc gtggatgcag tcggatatca ccgcgccggc aggactgacc ggcgcccatc
     2461 cctggacggt tatggtctgg gcgtatgcga cttcgacaac cggcgaggat tgcctgtttc
     2521 agtggggcga ccgcggcggg ggcaccgctc acttcaactt tggaacgcgg gcgattacct
     2581 tctggaacga tgtgttttat accaatgcac cggcgctgaa ccaatgggtg catctggccg
     2641 caacctatga tggaaccaac gtgcggctgt atgtaaatgg aacggatgat accgtagccg
     2701 ccagaagcct gaacatcaat accggcaact atatgcggat tgcccgtgcc atgccgaatc
     2761 tgtggggaac ccatccgttt ggcggcgcca ttgcttctat tcaggcctat gatgcagcgc
     2821 tgaccaagca ggaaattcag gtgctcacag gtttgtttga agccggttcg ctgagtccga
     2881 cgggtgtatc ggtgcgggaa aatggtccgg catccacaat ctatgtacaa atcaacacca
     2941 atcctattac cggccaagga cctcaaacag acttggaagt aaccctgagt ctgatcagca
     3001 cccagaagga tattcagctc ggtacgtccc ctgttggaca gccctatgtt ataacggttc
     3061 cggccgcatc ctatcagacg cctattccaa tcagcgtgct ggccgaggac gacgatatta
     3121 ttgaaagtca gcatgcggtt cgcgtcaggg cggttgttac tgcaggcgat ccggactatc
     3181 tgggcaagat tcttgtgccg aataacggac ttgcaatcac ggttatcgac aatgattata
     3241 ccccctgtac agcgcccggt ttgaccaacg gcgcttatgt ggaccacttc gattgcaagt
     3301 gggattacac gcagggcccc agctcgatgt ggagcggtct gatggataca gccggctata
     3361 cgacggatgc cacctcctcg atcagcgctg ccggcgcttt gcgtctggca tcggccaatg
     3421 ccttctggag cggcaacgac accacgggac cgtacctcta tatcgatgtg acaggtgatt
     3481 ttgaagtgga aacgtatatt tccgaatacg ccaacagtat tgccggacag gacggtgtcc
     3541 tccataatgc cggcggtatt atggttcgtc tggccgatcc ggcagccggc ggtgcgggcg
     3601 aggataatat tcaggccagc tacttcccga tttggggctg cggcaacatc ctctggtatc
     3661 acaacaacgg cgcccggacg gaaacggata ttgcagacac cgcctgggac ggcagccgtt
     3721 atctgaaggt tgtccgcaaa ggggcagcgt tttactgcta ccacagtccg gacggcaata
     3781 cgtggatacc gttcccgtcg gccaatccga tgattcggca ggatatgaat gtgccgacgc
     3841 tgcaggttgg attgtatcag tgcaccttca acaatacgct gggatttgtc gattatgact
     3901 acttctctct caagaaagtc agaggccatc tgaccggcac gctcaatctg accgaatgcg
     3961 atgctgatgc cggtacggtg cagttccagt ttgagaccga cggcgtaaaa gcacccatag
     4021 ccgatattaa cgtgaccgtt tatgcggtcg ccgcttctgg agccgacccg aactctgatc
     4081 ccaacgatgt agtcatcggc acagcggaaa tgggccagcc ctatacattt acggtctcac
     4141 cggcccaata cagtcagccc cagttgattc aggttgcggc tgtcgctgat gccactccgg
     4201 agccggatca gctgctgacg ctgcaggcgc agattgccag taccgatccg aactggaatg
     4261 gactgcttat cagctccgat gttctgattc atgtgatgga aacgccgggg cttctgctgg
     4321 ataccggcga tggagtccgg gtggctgaag gcgacggacc ggatacattc accgtccgcc
     4381 tgaagattcc gccgctggct tggccggttt cggttaacat aaccaacgct gccggtcagg
     4441 taacagtcga tccggcgcag ctgagttttg atgccgccag ctggaagacg ccgcaggttg
     4501 taacagtatc ggctgttgat gatgcggtcc ttgaagcgga tccccatgca acaactctga
     4561 ctcttacagc cgccaacggc aatgaatacg atgcattggc tcccttgaca gtgagcgtca
     4621 gcattctgga aaatgaatgc ggtgcttggg ggtatgtgtg gggcgatttc aacagggact
     4681 gcaaagtcga tgttgcagac ttaagcgaaa tggcagcagc ttggcttgac tgcaccaatc
     4741 cgtacgatac gggatgtgtg gatttacgct gattggcagt ttcttcggcc gggagcatgc
     4801 cgctcccggc ctttttttct ttttaagcgg gggtgtctat tcggccagct gcagggaagc
     4861 cggcggctga aaactgccgg ataggatttg cgttgtccga cagcagcggg tattatattg
     4921 caatacttca atttctgtat taaacagatg catgcggtgt tggtgccgca ggaaactgcc
     4981 ggattggatt tacagcggaa gaagtgactg gaatagttta aagattctgg taaaatggca
     5041 atctgtattt cagataagga gatcagctgt gtttaaggat gccggggaaa tgggagtttc
     5101 aattgtgttt gcagcggcag tattttttca agcaatcgtt ggcgcccaga tgaatgaaac
     5161 aataggctac cagaaagaac agcttcttcc ggctcggccg cttgagctgt caaaagttcg
     5221 gctgctgggc ggaccgctca agcaggcaca ggacaaggat gccgagtacc tgctgaatct
     5281 ggaacccgat cggatgctag cctattaccg ccggcaggcg gggcttgagg gcaaggccgc
     5341 tccgtatggc ggctgggatg gcggcggacg caacttgacc gggcatattg cagggcacta
     5401 cctttcagct gtcagtctga tgtgggcggc caccggcgac cagcgtttca aagaccgagc
     5461 cgattatatt gtcaaagaac tcaaagaggt tcaagacaag cacggcgacg gctatttgtc
     5521 ggcattggaa ggcggtcggg agtgctttga agcagtccgc cggggccaga ttcgttcagg
     5581 cggttttgac ttgaatggac tctgggcgcc gtggtatgtg ctgcacaaga cctatgccgg
     5641 cctgcgcgat gcctatcgct atgcaggcag caagaccgca ctggagatgg aaatcaaatt
     5701 tgccgcgtgg gcggaaggca tcttggccga tttgaccgac cagcaggtcc aaaaaatgct
     5761 caatacagaa ttcggcggga tggaagaggt gctggcagac ctgtatgcgg atacagggga
     5821 catccgctgg ctgaagcttt cacgccggtt tgagcatcat tcgataacag aaccgctcaa
     5881 gcgtcatcag gatattcttt ccggcaaaca cggcaatacc cagataccca agctgatcgg
     5941 atccgctgac cgatttgtgt atgacggtca ggccggtgat ttgctggcgg ctgccttttt
     6001 ctgggatgcg gtcgtccagc atcacagcta tgccaccggc ggacacggca aggatgagta
     6061 ttttggcgac cccgaccggc tcagtagccg cattgacggc cggacggcag agacctgcaa
     6121 tgtttacaat atgctcaagc tgtcgcgccg tctgttttcg ctgcgtcccg atacgcatta
     6181 tgccgaattt cacgagcggg cgctgtttaa ccatatactg gcctcgatcg atccggaaga
     6241 cgggcgaacc tgttatatgg tgccggtcgg gcagggggtc agtcatgagt atcaggatat
     6301 gttcggcagc tttacctgct gtgtcgggac gggaatggaa agccatgctc tgcatgccga
     6361 cggcatttat tacgagtccg gcaataagct gtgggtgaat ctgtatgctc cctcgactgc
     6421 ggactggtcg ggcataacca tcaaaatgga aaccgatttt ccggaaggtg attcggctgc
     6481 gctgacgatt acagccgaac agccgcgtcc ctttgtgctg gcgctgcggc gtccggcgtg
     6541 ggcgggggaa ggatttgctg ttgcggttaa cggaagccct gtggacaatc ttgcccagcc
     6601 ggcatcgtat gtggaactga atcggacatg gaaaagcggc gaccggattg aattaaccct
     6661 gcctaaacgg ctgcatctgg aagctacgcc cgataatccg cgccgcgttg cgattctctg
     6721 ggggccgctg gtactggccg gcgatcttgg ccctgagcag cgccgccagc gttcagcacc
     6781 gcgcgaagaa tccgaaagaa ttccggtttt tgtagcggct gaccagccgg tcgagcagtg
     6841 gctcaagcct gtggaaggcc agccggggca tttccgcaca gccggcgtcg gcagggaacg
     6901 ggatgtggat tttgtgccgt tttataaact gcatcggcga acctacggga tttattggga
     6961 tttgtttaca gaatccgaat ggcaggccaa ggcggctgaa tatgcggcag cccaagaacg
     7021 ccggcgcaag ctcgaacagg cgacggtggc ttatgcccag cccggcgaga tgcagccgga
     7081 acgggatttc aactatcagg gcgccgacga tgtccgggtt gagcggttag ggggccgtcc
     7141 atgccgggca ggcggaagct ggttttcatt cgatatgcct gtcgagccgg aacacccaat
     7201 gacgctgata gcaacctatt atacagggca gtggctgcgt cagccggctg tctttactat
     7261 tctggctgat ggcagaaaga tcggaaccga aactgtcgtg cggcgcgacc cgtatgagtt
     7321 ttatgatgtt gagtacctaa ttccgccgga tcttgttaag gataaaaaga agataacgat
     7381 acggtttgag gcggcaccgg aaagccgcat tgctgctgtt tttggcttgc gtatgattcg
     7441 tgctgatgcc tcgcgctaaa ggctattaac aaaagcagaa atgacaaggt aaggagacta
     7501 tcaatgaagg caatgcaatt gttgttttcg gccggcctgc ttggcatgat agggtactgc
     7561 atggcggctg aagagtctgc ccggccggaa attaatctgg ctgtggtggc ggttccatcc
     7621 agttcttatg tttcaggcga tacgagtctg gaagccctca acgatgaagc ccagccccgc
     7681 agctcgcggg accggcgcca aggggcctat ggcaactgga accgcaccgg tacgcaatgg
     7741 gtgcagtatg attggagcca gccgatcagc acccggaaga tagatgtata ttggtgggat
     7801 gaccggcagg gtgtccgcct gcccaaggcc tgccgcctgt tctattggga tggtcaaaag
     7861 tttgtgccgg tcagcaatcc tgtggggttg ggaattgccg aaaaccagtt caacacgaca
     7921 accttcgatg aagtgcagac aactaagctg cggctcgaaa ttgattctga tggaagctat
     7981 tcaacaggga tactcgagtg gaaggtttac gattccggcc ggtcgccggc ttttgcgccc
     8041 cgggtgtcag ctggtgtgga cagagtcgtg gtgctgggcg gccgcaccta tctgaaaggg
     8101 tcggtcaaga cgctggacgg ccggaccgaa accctccgat ggcaaaagtc cgaggggccg
     8161 ggggatgttg tttttgagga tgcttcaaaa tccgataccg cagcggtctt ttcgaaagcc
     8221 ggcgagtatg tggtgacgct taccgccgga aaggagccgc tgacagcgtc ttcatctctt
     8281 cgggttaaag tgattccggc gccgcccaaa gaacccctcc gggcggtgga tacccatcgc
     8341 tatactatcc aaagtccgct atggaacagc cgtgccaagg cccttattgt taattggatt
     8401 cctcactgta tccggaagat ttcagatccg cagctccggg aaggcggcat caacaatttt
     8461 attgaagccg ccaagaagct ggctggtcag gaacacggcc agcataaggg gtatgtattt
     8521 tccaacgctt gggtttacaa taccatcgaa tccatctgca ttgcactgca gattgatccg
     8581 cagggggatg cagatataat cgctgcccaa aagatgatgc aggaaactct tgaggactgg
     8641 attccgaaga ttttggcggc tcaggagccg gacggctatc ttcagacagc ttttacgctc
     8701 agtaatcggc agcgctggtc gccgcggttc agaggggacc atgaaggcta tgtggccggt
     8761 tattttatcg attgcgctgt tgcccattat gagatgaccg gccggaaaga tacccgcctg
     8821 tatgatgccg cgatcaaatt ggcagattgc tggtacaatg ctatcggtcc ggctcccaag
     8881 caggaatggt atgatggcca tcaggcaatg gaaatggctc tggtccgtct ggggcggtgc
     8941 gttaatgatg cacaaggcca gaacaaaggc agcaaataca ttcagcttgc caagtttctg
     9001 ctggactgcc gccgagacgg cagtgaatac gaccagagcc atctgcctgt tatccagcag
     9061 tatgaagctg tggggcatgc tgtccgggca gtctattctt atacgggcat ggccgatgtg
     9121 gcattggaaa ccggcgatat cgactatcaa agcgctgttt tttccctgtg ggacaacatc
     9181 gttaatcgaa aatactatgt aaccggcggc atcggcagcg gcgagacatc cgaaggattt
     9241 gggccgaatt actccctgcg gcacggcggc tactgcgaat cctgctccag ctgcgggcaa
     9301 atctttttcc agcaccggct gaatctgatg acgcacaacg ccctctatgc cgatttgtat
     9361 gaagagaccc tttataatgc gctgctgggt tcggtggact tggaaggccg caatttctac
     9421 taccagaatc ctctgatttc acagggcaga cgctatgact ggcatgcctg cccgtgctgt
     9481 gtcggcaata ttccccgcac gctgctgatg cttccgacgt ggatgtatgc aaagggaccg
     9541 gatgctgttt atgtgaattt gtttgtcggc agcacggtga agctggaggg aattgcggga
     9601 accgatgttc agatggttca aaagacagac tatccgtgga aaggaactgt tgagattaca
     9661 gtcaatcctg tcagccaagc cgaatttacg gtttttgttc gtgcgcccca tcgcagtgtg
     9721 agcgaattat acagcgacat tccgcctgct gacgggattg cttctttgtc ggtcaacggc
     9781 cagaaaatca atccggccgt tgagaacggc tatgccgtta tcaagcggaa atggaaagcc
     9841 ggcgaccgga ttgaattggt cctgccgatg cagattcagc ggattaaagg aattgacaaa
     9901 atcgaagcaa cccgcggaca agcagcgctt cggtatggcc ctgttgttta ctgctgcgag
     9961 cggattgatc agccgctgga caaagtgctg ccgccggatg cggctcttga aaccgaatgg
    10021 cgggcggatt tcctgcaggg cgtgatggtc atcaaaggaa gctggtatga tggaacgccg
    10081 cttttggcta ttccatatta cgcacgggat aatcgtttcc cggatgaaca gtcccgtccg
    10141 cgagaccgcg gtcctgcctc ttctgtatgg cttaaagaac gctgaagcac aatgtccctg
    10201 cataaaaagg gcagcccgct gatgggctgc ccttttttta tgcgtttggt cttgcagccg
    10261 gcttaatcga tatgaaacac ttctgtcagt tctctggact gggggctgtt gtcgggattg
    10321 gagggcataa tatctttcat ataagcccac catttttggc agatgccggt ctgggcaatt
    10381 gcatcccatc gtttagggtc ctcaatctcg acatagccga ataattgact cgtttctttg
    10441 tccagaaaaa tagaatagtt gtgaacaccg tggtctttta atgtctgatg cagctgtttc
    10501 cagataggac tatgccgccg ctggtattca tcctgatatt ccggatagac ctgcattcga
    10561 aaggcttttc gtatcattgt actttgcctc agcttggttt ttgattctat tttaggataa
    10621 atgcccagat ttgtcaaaac cggaaaaaca atggaaatcc gcaggatttg ccgataaact
    10681 tggtattcga gctgtggtgc gaaaaagccg attgcggaga acaaggatga agacgacaat
    10741 taaattattc acgggtttgg cagggattgt tcttgttgga gcaggaggcc ttttcctgtg
    10801 gcatcaggta tcctctaagc ggacaataga gcagcttttg cgggaaaatg aaactcttaa
    10861 gacggctatt tccaacctga cgcaggaaag ccagatcggc tatgcgaagg tgctgtcgca
    10921 ggagattcgg gacggcgcag tatataccaa actgctgttt gttgaggttg atccatcgga
    10981 tttcacaaag caccttctcc gcagggaata tgaaattgaa ggcgatgttg tccatttcga
    11041 tacactgatt gttacattcg gcagtcagct ggtgatggac ggcaaagagc gtgcgatgta
    11101 tctgtggcgg cgcatctacg gcgagaaaat gccgcccgaa cagggatttc ctatcgagca
    11161 agagggtcag ccttcccccc gatacagtga actatgtgca aaattgtcaa tcgaagaccg
    11221 caagctgttc tgggatgaaa tctggtcgct ttccaataat cccaaacgtc tggaaaagct
    11281 gggcatcaag gcggttttcg gcaatgcagt ttatcgccag ctcaagcccg gtttaatcta
    11341 tgtgttcaag atttcgccga cgggttcgct ttatccagaa atcgtaccgg atttataaga
    11401 gattttgaaa tacctaacgg atacgaatcg gagaagaaga tagactggat atgtaagggt
    11461 gagattatga aaattcaaag tgtcaagggc ctcatttgtt tattgagtac aattgggctt
    11521 tcttgtgctg ctttttccca gcaggacggt tgcccggaag gaaagcttgc cccgaagccg
    11581 ctttatcgag accccgtgta tgatggtgct gcggatccgg ccttgtgctg gaatcgggct
    11641 gagcagaagt ggtttatgct ttatacaaac cgtcgggcca atatccagaa tgcagagggt
    11701 gtaagctggg tgcatggcac gcccatcggc attgctgaat catccgatgg aggcgccaca
    11761 tggacctatc gatgcgatgc gaatatcggc tatcaaaagg gcagcgatac ctattgggcg
    11821 ccggaggtta tcgagcataa gggtctctat catatgtatc tgacctatgt gccggggatt
    11881 ttcagtgatt ggaaccatcc gcgggatatt attcatctga ccagcaaaaa cctgattgat
    11941 tgggagtatc aatccacgct caaattgtct tctgacagag tgattgatgc ctgtgtggca
    12001 ccgctgccgg aaggcggctg gcgtatgtgg tataacaatg aaaaagacag aaaatctatc
    12061 tattatgccg atagtccgga cctttatgta tgggaggata aaggcaaagc cgtcggcgac
    12121 cgggcagggg aagggccgac agtttttgac tggaaaggcc atcggtggat gattgtggat
    12181 aactggcagg gactgggagt ctatcggctc caagaggatg gcagctggct gcgtcagtcg
    12241 caaaaccttc tgcagacgcc gggcagggga aatgaagacg gcgtgattgg acagcatccc
    12301 gatgtgattg tcagtgatga acgggcttac ttgttttatt ttacccatcc cggcagaaca
    12361 ggggaaaatg cccgccggga tgcttatgag cagcggcgaa gctcaattca ggtagtagag
    12421 ctggactacc gcgacggctg gctgacctgc gaccgtgaca agccgacctg tattatgctg
    12481 aaagctccgg cgctggaatt taatgtctgc gattatggag cagtcggaga cggccaaact
    12541 gtctgtacgc aggctatcca gaaagcaatc gatgcctgtg ccaaagccgg cggcggaacg
    12601 gttgtgctat ctgttcccgg aacatatatg accggttcga tttttcttaa agacaatgtc
    12661 aatctgcggc ttgaccgggc ggttgtgctc aaaggtgttc aggatgaatc ggcctatccg
    12721 gaagtctata cccgcgtagc cggcattgaa accaactggc ctgcggcttt ggttaatgcc
    12781 cacgacttgt cgaatgtcaa aatttacgga cagggtacca tcgacggatc cggttccatg
    12841 tggtgggata aatactggaa tatgcggcgt gaatatgatg ccaaaggact gcggtgggtg
    12901 gtagattacg actgcaaacg gccccggctt atcctgattt ataatgccag caatgtttcc
    12961 ctcaaagacc ttactttgca ggagccgggt ttttggacag ttcatatctg ctacagctcg
    13021 catattacag ttgatggtct gataattcgt gccaaccttg atgagcgggt cggcccgagt
    13081 tcagacggga ttgatattga ttcttcgaat aatgttctgg ttcaaaactg cgatattgac
    13141 tgcaatgatg ataatttctg cctcaaggcc ggtcgcgatg ccgacggctt gcgtgtgaat
    13201 cgtccgacgc atgatgttgt gattcgcgac tgcattgccc ggcgcggaca cggcctgatt
    13261 accttcggca gcgaaacctc cggcggcatc tacaatgtgg aagtctataa cctcaaagga
    13321 tacggcacca gtacgggcat tcgctttaaa tcaacccaag ggcggggcgg tactgtcaaa
    13381 gatgcctata ttcacgatat ccaaatggtc aatgtccggg atgccatcat gctggattac
    13441 gactggtatc cggcttataa cactgttccg gatgcagttc gccggcaaat cgaagccgaa
    13501 ggcaaagaat tgccgcgaca ctggaaagcc ctgatggaac aggttcccga agacaaaggc
    13561 acgccgtata tccgtgacat tcggattgcc aatatccatg ctgtggattc gcagacggcc
    13621 attaccatca gcggccgcag gaatcagcgg gccggaaact ttttactcga gaatgtccgt
    13681 atcactgcca gcaaagcagg cacgataaaa aatgctgaaa actggacatt caaaaatgtt
    13741 gtcatcgaag ccaaggacgg ttcgacggtc aaatttgaaa attgcgagaa tatgaccggc
    13801 ctctaaaatc ccaaacaaga aggcctctat tgaataacac aatagaggcc ttcttcgcgt
    13861 attgtctcta tcagacaata caccctcctc ttcctgcctt tggtttcatc tatgcaaact
    13921 gttacagcgg ttcttataca acgccgtatt gttatattgt ttttgctgta tcagcgtttt
    13981 ctgctgagca gtagtccgcc caatcccagc agaaccagcg tcgccggttc gggaacgata
    14041 acaagagttg gccatccgcc gtttgcatct tctttggagg caaatcggtt ttcgctgttg
    14101 ttggaactcg tgcctgcagc gttaataagc acaaacgtaa ccagaccatt tgtgtctgcg
    14161 ttgagaaaat ctgccagagg cagagtagcc ggatgcgtca gaattgttac cggcggtgtt
    14221 agagtcgtgc ctgaactgct gccgaggccg ggaatgataa aagttcccaa caaaatcagt
    14281 cttgcgggat cgatggcata atatcccaag ttgtttcctt cgggaggtgt aagaaatccg
    14341 ggagcgttat cataacagag tgtggtttcg ctccacaatt cgccggcatc gccatcgacc
    14401 aatccataga catgcataac tttatcactt cccttggtaa atgtctgtcc cagctgcagt
    14461 ttagcgtctg cagcgatagg tccggagaca gaactgatgt caaagcgcat aaaagttgct
    14521 ctgaatcgtg aaccgccgcc gttataacga gcctgcatgc cggttgtatt atggacagta
    14581 ttggaccaat aggaactgct ttggttgtcg ttggaaatcc aaccgtcagc cccattgccg
    14641 tctgctgttg tgataagcag agcctcaacc cctgtgccta accagatcgt taacaacaat
    14701 acaattactt tcttcatttt tccattcctc caaaattcac acatacactt tcttaaaagg
    14761 gtgggcagct tacactgctg cccgccgaaa caccacctac agttcaatca ggggcacaca
    14821 taactgttgc agtcaagcca ttgtgctgca aatgcggcga agtctttcag gttcaccatg
    14881 cagtcgcggt taaagtctgc agcatttgcg gaataacccg gcgtattctg tgccgcatca
    14941 caggggtctg tgccgacata aacctgtacg gtatcggaag ctgtcatgtt ggaatcatcg
    15001 gctgtcaggc ggaacacata ggtgcctaat aacggcaaat acacagttgt ttccagcgta
    15061 tcagccggat caatggttac agtcgaagga ccgctgactt gtgtccactg aacggtgcag
    15121 gcggccgggg gatccggcag accgtcatcg gttacagtac cgctgagcgt aacagctgca
    15181 tcgccgttgt tggcagctga atcgggcaca taggtcagtg tcagattcgg ccgatagtcc
    15241 ggattggaat tttctttgga gccgacgccg aatcctctgt cggtgccgtc gtcattggct
    15301 aaaataaatg tgaccagtcc attgtcgtca atacggcttt ttaagaatgc ttccagcggc
    15361 gggccggaga tagtgatttt gctgccgacc gctgagccgc cgattgtttc gacgattcct
    15421 tcgacattgt cgtcgaggtc tgtaatccag ccgttagcca gcgctgtagt catcggcata
    15481 gtaccgttcc attcttgacc ggtgttggta tccatcaaaa ccgcttcact ccagttttgg
    15541 ggggtgttgc ccggaacatt attcaggccg tacagagcga atcgggcatt ggtgggcgtg
    15601 tcgttgcggg aagcgccgcc tgaaatggtc agggtcaagg taacattcga aatcacaccc
    15661 ggtcccattg ctgtaagagc ggataaatcg aaccggagat aaccggcaaa atcagtaccg
    15721 cccccgcgta tgtcgataac aggttctgcg ccgcgaaccg tcgtatcacg caaataagta
    15781 tcggcatcac tggtcgtatt gacgacagct ttggtcagcc aaacatactg gtctggtccg
    15841 gcattgacca ccggtgcggc attgttggtg tcaaataccc agacaaaacc cggtactgtg
    15901 cgatccggga aggaactgtc gtgaatgtca acaacccagt agtacgttgt aaactgcgcc
    15961 agcggaggga tagcaaccga attacctatt acgccggcct gcagcagggg aagtccccaa
    16021 tgcggctcca gtgcattcgg ctcggttgtg ccgaagtaaa tgtcacaggt aattgtctcg
    16081 cccggattgt tgggttcggg gttggtccag cttaacatat tgaggcctgt cgaaaccgtt
    16141 gcataggaag ccggaaccgg tgctgttgca cgggcaacaa tatctcattg gaatccggca
    16201 gaaccagtgt cggaaaggta atgcccgcgg tggtattttc ttttgtagca agttcggtct
    16261 catcatcctg cccaatcagc agcagtgtca ccaatccatt ggtgtctgac cggagaaagc
    16321 tggccaaatc cagattggtt ggattagatg taagaataac tggagcggcg gtagtagaag
    16381 tctggaatgt gcccaacagt ttggttttgg ctgtgtcgag ggtataactg cccggtgccg
    16441 ccggcagcac ttgaccggga gcggtattga aggtaatccc gccggtgccg ctttcaatcc
    16501 agtaatccag cctgtcatct gtcaatccat aaacgtttac cgatttcccg ccggatttca
    16561 tatatgttgt ttcaaatttc aaataggcgt ccgtcggatc cagcaggacg ttgctgaggt
    16621 cgaagcggat ataggcaatt ttgacacggt ttgtaccgtt gacgcggata cgcatgcgcg
    16681 gttccgttcc aaaattgccg tcgggacttt ggctgccgtc atcattggct acataggtat
    16741 cggcaccatt gccggcagct gttgtgacgg ccagttctgc atgggcaacg agccccaaca
    16801 gactcagcaa aacaattgaa aacaataaat ttctcatagc ggaatcctcc ataaaatgtt
    16861 aataaagata tagaagatct gtatatgcaa aattgccatt tacacagcaa tatcttcgaa
    16921 gtgaaatatg ccttttgtta atacccctcc gtatgctgtt cattcttacc gcaatcctct
    16981 tcatccgcct cacctttccc ggcactattt acacgggcat tgatattagt caatcattac
    17041 atttggacat agtagattta atggtttttt acgcaaaaag ttataatttt tttaggtttt
    17101 attggaggta tatcaggtat taattgtctg gatacatgtt attaatttag tgcgccaggc
    17161 attattcgat aaaaaaaagc gggcagcaca aaaagctgcc cgcctaaaag aaattactaa
    17221 aaaccaaata cttttacagt tcatcgcatg ctgttacagg ccataaaccg cattccagcc
    17281 aagacgaggc gtaaactgta aaatcttcca aatcaaccac acagttgcca ctgtaatcta
    17341 aaacaggttt gaagcggcag taaggaccgg ctacatccga ataaatggta gccagtgtgt
    17401 cggcatccaa cggataattg taaatccgta tctcatcaat caagccattg tacagagcat
    17461 ctgcttccca gttgctttcg ccgatgaagt tgccggttcg agtaacataa ttaatcttct
    17521 ttgagatact gcctgtctga atcgagaagc cgtctttata cagcaccaca ttccctgcct
    17581 cgtccacagt ggccgcaaac atttgccatt catttagaac caaagcatta tttgctgtga
    17641 cagctccggc actggcatta ccctcgtaac agttgaaggt caggttgctg gttgtcccgt
    17701 ttcgagtcag gaagatgtta tcgctgttcg gtccgttacc caaatcaata aaccgtgccc
    17761 agctggttac agctgccggt ttggcccata cagtaaaggt caggccggcc cggaaatttt
    17821 cgaaaccatc gggcaggtca acatagtcat tatcaccgta aaattccatg gcatgatgat
    17881 tgccgtcgat gacaaagttc ggctcgccgt agattgtgcc gttgtagaca cctgtctggt
    17941 caacggcatc gccgttgaac ccccactgag ccagcaggcg tttgactgcc aaatatcccg
    18001 gtgcggatgg ttcggaattg ccgcccgaat tctgc
//
LOCUS       DAOTBO010000057        17750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2633_length_17750_cov_5.638146, whole genome shotgun sequence.
ACCESSION   DAOTBO010000057 DAOTBO010000000
VERSION     DAOTBO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 17750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17750
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_17750_cov_5.638146"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PLE88_07780"
     CDS             <1..1113
                     /locus_tag="PLE88_07780"
                     /inference="COORDINATES: protein motif:HMM:NF027673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOM76401.1"
                     /translation="ETGSGSWITHHVPYGTVRWEVDKFEPEEYNELFAEFRFQTIFRN
                     NPSGHKMFLWWNFREFYNKKFKNNRNTKGILTLAGMPKDYYYLYQAFLQPDKPVLHLC
                     GRHHFCRQFAPDNGIKAYSNAASAELFVNGLSQGIRRNGQYVQPDSVKRSGSGTETVK
                     GIAVNNVFFWNTALQPGKNLIEVRDNRGGRESMVIYQKSAEGTMPESPDSLVVNLTSS
                     NPDNPAFFIDRPAEAQGPFYTDVDGSSDNTFDLLPAPLEGARWIATRRLSEPKNKTAL
                     SFSLRRPATVYVMYATGTFPAHTLRKPDASIQNAAAQLEEALQKAGFDDAGIRGIWRG
                     HDLWLADYKAASRRVEPGQTISIPAQTLDYVVLVKE"
     gene            complement(1116..2720)
                     /locus_tag="PLE88_07785"
     CDS             complement(1116..2720)
                     /locus_tag="PLE88_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76402.1"
                     /translation="MPKQNRKTKRTLKAVFQILCLALLTGVLAVRPAWAEQRAVSEWI
                     YPGPDGKLVYKTTPAGDRIMDFSHAGYKGGGVPLPTVPVKQTVRPTGGDDTAAIQSAV
                     DAVSAMPLEDGFRGAVLLAPGTFTISRTITIAADGVVLRGSGEQGPACSTLQLTGRPF
                     TAVAVRRTAGRRPRGDIQPPEGVQTEITDAYVPSGAQSFTVADVNGLAVGDTILIRKP
                     VTEAWVKFMQMDDLVRDGRPQTWLAVGSTVDAERRIAAIRGSRITVDVPLSDSYDSKW
                     LNPPGVKVVKITPPQRVSNAGVEFLHIQSPPQPISHTQPHFSALRISGQDCWARNLII
                     DETMNSVGASGCRITLEKVSINRKARHQGSSRPAEFAPNASQILLNRCSVNADNVWFV
                     ATGAQVSGPIVILNADFRGDSRAEAHQRWSTGLLFDNVTAPDGSLELRNRGSMGSGHG
                     WAMGWAVIWNCTAKDYIVQNPPGAVNWVIGSVGQLKTAARPFASRPDLPAAIVDAHAT
                     PVVPQSLYLAQLAERLGPQALQNIGY"
     gene            complement(2836..3705)
                     /locus_tag="PLE88_07790"
     CDS             complement(2836..3705)
                     /locus_tag="PLE88_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOM76403.1"
                     /translation="MENAVQKTIGRLKSLMNPCRLCPRQCGVCRSEGQTGFCGIGGMA
                     VVSSAGPHFGEERVLVGSGGSGTIFFAGCNLRCLFCQNFDISHFRSGVPATSRQLAQM
                     MLQLQDSGCVNINLVTPTHIVPPIAEAIVLAKEQGLHLPIVYNCGGYESVETLRLLEG
                     LVDIYMPDMKFSDSAVSRQLADAADYPQRNRCAVQEMHRQTGDLCIENGLAVRGLLVR
                     HLVLPNNLAGSMEILDFLAEQISPATAVNVMDQYRPCFQAGSHPRLNRRPSSNEIESV
                     VRYAAAKGLTIIE"
     gene            complement(3715..4971)
                     /locus_tag="PLE88_07795"
     CDS             complement(3715..4971)
                     /locus_tag="PLE88_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOM76404.1"
                     /translation="MSNIFALVDCNNFYVSCERVFNPRLENRPVVVLSNNDGCIVSRS
                     DEVKALGIPMGKAAFEVRDILDKHHVEMLSSNYTLYADMSSRVMQTLSEFTPNIEIYS
                     IDEAFLDLSGIAGSLTAYGRRIQQTVRQWTGIPVSVGIASTKTLCKLANKLAKKSSKA
                     AGVLNLIDSPYLQEALRRAAAADVWGVGYRTAGKLGKAGILTALDLSRADIEWIRQQF
                     GIEGVRTVYELRGIACYPLQENPPSKKSLAVSRMFGRPVESFDQLSEAVASYASRAGE
                     KLRQAHLAAGVLTVFAATSRFIQKRYFNACTIEFPVQTSDSVELIRASLDALGKIYRS
                     GYQYKKAGVLLHHLGDEAKVQKCLFDAADRDKSRRLMRAIDAINARSGCSICWGAEGL
                     EKPWRTQFNRLSPKYTTRWDQLPVVS"
     gene            complement(4968..5399)
                     /gene="umuD"
                     /locus_tag="PLE88_07800"
     CDS             complement(4968..5399)
                     /gene="umuD"
                     /locus_tag="PLE88_07800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOM76405.1"
                     /translation="MKAKVSAIYRSDISTAYRQPYFEAKVPAGFPSPAADYEENQLDL
                     NKHLIKNPAATFFVRVSGDSMTGAGIHDGDLLVVDRSLEPKDKNVVIAAVNGELTVKR
                     IRIRKKKVFLEAANEKYAPRQISDQTDFQVWGVVTSVIHPV"
     gene            complement(5486..6346)
                     /locus_tag="PLE88_07805"
     CDS             complement(5486..6346)
                     /locus_tag="PLE88_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOM76406.1"
                     /translation="MTQRVSIGVAGWSYPDWQGIVYPAGLKDPLGYLCRYVDCIEINS
                     TFYRQPEEKSCRGWLQRTQTKPDFFFTAKLHRDFSHEGRIEPDMVRQFHRGLAPLLEA
                     GKLRHLLIQFRYDFDDRDGHRDHLQKLAEQFGGQFSIVVEVRHRSWEKADALNFLKDL
                     GVIVANIDYPVGRDSFNLQTCAVGTTGYFRLHGRNAEKWFSKSTRDETYDYYYSTQEL
                     AEIQDRIRKLMALYPSVVVITNNHYRGSEVANALELKAALTGRKVLVPEDLMLRYPQL
                     EKIALNRPLF"
     gene            complement(6413..7135)
                     /locus_tag="PLE88_07810"
     CDS             complement(6413..7135)
                     /locus_tag="PLE88_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOM76407.1"
                     /translation="MHDAGDRFQKETKYRPDTMPAGSSAGAVKPKLYKTYPDSPQIKL
                     PDVRALSAMPLSEALKNRRSIRRYQNRPISAEMLSYLLWAGTGISRVEGGYEFRTAPS
                     AGALYPIETYLAANKVADIPAGLYHYSIRNHSLELLGEGDYGGRLARAALGQGMCASA
                     SAVFIWTAVFERTKQKYGQRAYRYIYLDAGHIAQNLALAAVSLGLGSCPIGALADDEV
                     NQIIGADGTEESVLYMSTVGWF"
     gene            complement(7214..8989)
                     /locus_tag="PLE88_07815"
     CDS             complement(7214..8989)
                     /locus_tag="PLE88_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOM76408.1"
                     /translation="MIYITAILVYLAGLLFVALRQVRKVKTQADFSVAGRTLSVWILV
                     LTMLATWVGTGSILGNAGKAYAIGLGAMILPVGPALGMILLVFIAPKVRNFDTFSVPE
                     IIEARFGFTARVMAVLALVAAYLVIVSYQFNAGGGVLQYAVGKKEPTVLSENQTVTAY
                     QLRKGYVYFVPSEGFTGRPEICLRPAGPESGDTITVALRVVPAEKVQEANIEIHQQGL
                     AGWIAAAPGQKVVLRPVQTAEGRYSVASISGGTLFLQEYKLSIRQATLIAAAFIIAFT
                     MIAGMVSIAYIDIGNGILIIGSLLAAFAYLWNKAGGWEGISTALAAMGRGENMRFWAH
                     LDGFSVINLCLPAFLLVLGDANLYQRFFSSKSAAGAKQATLVLIFAALLIEILIIGCG
                     WTAGALIPDAEEGKYVLIAASRYLMPPWLGCIFMTNIVAIIMSTADSFLLVPSASLIR
                     DIYKAYLNPSATEKQTLFLSRLMVLCLGAAAFLISMTFDRVPGFLSRALYAYTIYGSS
                     ITPALVAALFWKGATRHGAVASILCGTAMTLLWRKMTFMAALLGSYYTMLDEVLPAIT
                     VSVTVLVVVSWLTARRQTKTPLAAA"
     gene            complement(9007..10539)
                     /locus_tag="PLE88_07820"
     CDS             complement(9007..10539)
                     /locus_tag="PLE88_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76409.1"
                     /translation="MKKAALFLISAVLSFSIISSASDSAVKPSAENEITTPPSGYDPF
                     FSKYLDCGGIPVIGSSRVADAAFYRVRELLNSILANRPDIHQALADAGVKYVIVAHDE
                     QVTDIPMYSHMQPKEYWNERARGFGGLTTSCGEENLLNMPMDRYEDESIFVHELAHAI
                     HSPALQRIDPTFQPRLEALYRKAMDKGLYKYDYASTNPSEYWAENVQAYFNCDRQTNW
                     NHNEINTRQELAAYDPEMKDFIAEIFRFTDENAWSYVPLKKQPSVIPVPSDLETDPVF
                     RHYVWCRGFSIFGTAGVSEAALLEADRIVRNMFRYRHDVLKALIDGGLELAVYEDGQK
                     PANLTALNPAAFVDAQDPNRPAVTLPKRLRFGVPQSNLLDGLNSRPNEASMLIGAMAK
                     ALLLYTGFREVDPAYDSRRLKQQYEKDLERLDRRTYEKVRQLYQTAMEQGLWKGTAAA
                     QNAFDYFAAGVQAFFDGGSLTAADGQPLGTRQHLQEHDPRLEAFISGLFKHPERVDWR
                     LR"
     gene            complement(10933..11005)
                     /locus_tag="PLE88_07825"
     tRNA            complement(10933..11005)
                     /locus_tag="PLE88_07825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10970..10972),aa:Arg,seq:ccg)
     gene            complement(11136..11669)
                     /locus_tag="PLE88_07830"
     CDS             complement(11136..11669)
                     /locus_tag="PLE88_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76410.1"
                     /translation="MAKRNRNVTVLFCLIASMTVGAAVLMALDNHRPMTGAYSLSSYL
                     RLDPVEDAVRNTMSVPAGQWQEVEIFYSRTSGGNADELGLMMGLTTGSSPEFHFVVGN
                     GNGAGDGHIQASDLWKLQRLCHGRPGVVRICVIGDGSSNSVTDFQIKRTITLVESLVH
                     TFNISPQRVRYPLNWQM"
     gene            11988..12731
                     /locus_tag="PLE88_07835"
     CDS             11988..12731
                     /locus_tag="PLE88_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76411.1"
                     /translation="MSELDVIRQIAHQALTIPTLTGISDNWLWDRTLRILRTVEHICR
                     LPELVNRAAAVDRFCLISATYFADAGFAHYIDVENVSARLVLADISLAELCDFSTQVV
                     SDRLVGAMSGLRIDKINRIILESCNRQTSMIEAMILSDARNLEDMGAVGLFHELRRYV
                     IHGKGISEILDSWKRKIDYGYWQARLKESFRFEPVRLLAEQRFAAAEYFMTQLATENA
                     ARDLEERLLESLDFSKSADSAEPVASQPG"
     gene            12739..13260
                     /gene="coaD"
                     /locus_tag="PLE88_07840"
     CDS             12739..13260
                     /gene="coaD"
                     /locus_tag="PLE88_07840"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOM76412.1"
                     /translation="MKIEPITAIFPGSFDPITNGHLDVIRRGNKLFDRLIVAVGQNPG
                     KRELFSKSERVDMIRQLVAEMPRVSVESYDCLTVEFAKQKNAQVMLRGLRNLTDVEYE
                     FQLAMTNRTVAGIETVFVMTSEEYGYVNSTMVRQLAILGGDVSKLIPAAVYERLRQKI
                     QKNPHPANPDVPE"
     gene            13299..13664
                     /locus_tag="PLE88_07845"
     CDS             13299..13664
                     /locus_tag="PLE88_07845"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOM76413.1"
                     /translation="MYTIFVEMPFKAQHQLTFEDGTQEPLHEHEWKVCAAVSAEKLNK
                     EEMVIDFEELKSLLDNILQNFQGQQLETLGIFEHRNVSAENLACTIYKQLAPQLPNTV
                     RLRYIEVTEAPGCRARYRP"
     gene            13761..14258
                     /locus_tag="PLE88_07850"
     CDS             13761..14258
                     /locus_tag="PLE88_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOM76414.1"
                     /translation="MICQSCKEKTATIHLTEITNGHRSETHLCQDCARQQGLTIKTQI
                     PINELLNTLLSAQSKSQSTGSVSVQSGRACPVCGMTLKRFAAEPLLGCPYDYTEFQAE
                     LLPLIEQSHNGKSRHCGKTPARTSQQDRNEIMLIQLRRQLEQAVKNEDYETAAKLRDQ
                     IKMYQ"
     gene            14255..15313
                     /locus_tag="PLE88_07855"
     CDS             14255..15313
                     /locus_tag="PLE88_07855"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOM76415.1"
                     /translation="MRPAELCKYPSSWFGGGSDVVSEVVISSRIRLARNIAGYEFLPC
                     LSPARQRQLEDRLREILLSIDLGKKVFFIDIDQASDVERTLLTERYLISRRHALGKGP
                     RGVVIAEDEAFTAMIHEEDHLRIQVFAAGLQLNACWDRINRIDDAIEQKIDYAFDPRL
                     GYLTACPTNLGTGIRVSVMLHLPGLKMTGQMEKFITAARDCDMAVRGLFGEGTEATGD
                     FFQLSNQVTLGISEKQVVEDFTTHIVPRIIAYETQCRQALLEQRPQLLDDKIYRALGV
                     LRSACLISSQEALFLLSNVRLGVNLGRIKNIDIATINELFMLTQPAHLQIRAGRILDP
                     DQRDTLRAEIIRAKLNQN"
     gene            15324..16094
                     /locus_tag="PLE88_07860"
     CDS             15324..16094
                     /locus_tag="PLE88_07860"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOM76416.1"
                     /translation="MRTKHALQFCRLLGLLGAVLTAGISCVSPQHASAPGRAEPLDIL
                     VLTGGHGFEQQPFYEMFSAMEGLRTAFAELDQQSRYWETLQAGDADVIVMYHMRQQIT
                     DKAKANLLALCEKGTGFVVLHHSICAYNDWDAYRKLVGAAYLHQPRTIDGTVVEPGTY
                     KHDVAVPVHIAASHPVTAGLADFELFDEGYKGCWFEPDNTVLLTTTQPASDPVIGWVR
                     TFRNSRVCTLQSGHGKDTFDNPAYRRLVQQAIVWAAGR"
     gene            16314..>17750
                     /gene="dnaK"
                     /locus_tag="PLE88_07865"
     CDS             16314..>17750
                     /gene="dnaK"
                     /locus_tag="PLE88_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOM76417.1"
                     /translation="MAKIIGIDLGTTNSVVAVMEGSSPKVLINASGSRMTPSVVGFTD
                     KGERLVGQTAKHQQVTNPKNTVYSIKRFMGRRHKEVSSEEKMVPYTITGGPDELVKVS
                     IRGKEYTPPEISAMILQDLKKTAEDYLGEKVEQAVITVPAYFNDSQRQATKDAGTIAG
                     LKVERIINEPTAAALAYGLEKKKNEKVAVFDFGGGTFDISILDIGDNVFEVLSTNGDT
                     HLGGDDLDEILINYLADEFKKTEGIDLRQDPMAHQRLKEAAEKAKCELSTQLEATVNL
                     PFITADANGPKHLNITITRSKFEALAEPVFERLRQPCLKAIEDSKLKVSDISEVLLVG
                     GSTRIPKVQQIAQEIFGKVPNKSINPDEVVAMGAAIQGAVLAGDAGVKDILLLDVTPL
                     SLGVETLGGVMTKLIERNTTIPTSKKEIFSTAADGQTSVDIHVLQGEREFARDNRTLG
                     RFQLSDIPPAPRGVPQIEVTFDIDANGIL"
BASE COUNT         4386 a         5040 c         4487 g         3837 t
ORIGIN      
        1 gaaaccggct ccggttcgtg gataacccac catgtgccgt acggaacagt ccggtgggaa
       61 gtcgacaagt tcgagccgga agaatacaat gagctgtttg cggagtttcg cttccaaacc
      121 atcttccgga ataacccgtc gggtcataag atgtttttat ggtggaactt ccgggagttc
      181 tacaacaaga aattcaaaaa caaccgcaat accaaaggca ttttgacgct ggctggaatg
      241 ccaaaagatt actattatct ctatcaggcc ttcctccagc cggataaacc cgtcctgcat
      301 ctgtgcggac ggcatcattt ctgccggcag tttgcaccgg acaacggcat caaggcttat
      361 tccaatgccg catcggcgga gctgtttgtc aacggcctat cacagggtat ccgccgaaac
      421 ggccagtatg ttcagcccga ctccgtcaag cgcagcggca gcggaaccga aacagtcaag
      481 ggaatagccg tgaataatgt ctttttctgg aatactgctc ttcagcccgg caaaaacctc
      541 attgaagtac gcgacaaccg gggcggccgg gaatcgatgg ttatttatca aaagtctgcc
      601 gaaggaacca tgcccgaatc cccggacagt ctggtagtca atctgacaag ctccaatccg
      661 gacaatccgg ccttctttat cgacagaccg gcagaagcgc agggaccgtt ctacaccgat
      721 gttgacggct cttccgacaa tacgtttgac ctcttgcccg caccgctcga aggcgcacgc
      781 tggattgcca ccagacggct gagcgagcca aagaataaaa cggcgctatc tttcagtctg
      841 aggcggccgg caacagtgta tgttatgtat gcgacgggga ccttcccggc acacacgctt
      901 cgaaagcccg atgcatcgat tcagaatgcg gcagctcagc tggaagaagc cctccaaaaa
      961 gccggtttcg acgatgcagg catccggggc atctggcggg gacacgacct ctggctggca
     1021 gattataaag ccgcaagccg ccgcgtcgaa ccggggcaaa ccatctctat tcccgcccag
     1081 acgctcgatt atgttgtctt ggtaaaagaa taaactcaat aaccgatgtt ttgaagagcc
     1141 tgcggcccca gccgctcggc caactgggcc agatacaaac tctgcggcac gacgggcgtg
     1201 gcatgtgcat cgactatagc tgccggcagg tctggacggc tggcaaacgg acgggctgct
     1261 gttttgagct gaccgacgct gccgatgacc cagttgacgg caccgggcgg attttgaacg
     1321 atgtagtctt tggccgtgca gttccagatc actgcccagc ccattgccca gccgtggccg
     1381 gagcccatcg acccgcggtt ccgcaattcc agactgccgt ccggagctgt tacattgtca
     1441 aaaagaagtc ctgttgacca gcgctgatgg gcctccgcac ggctgtcgcc gcgaaaatcg
     1501 gcattcagta tcacaatcgg gccggacacc tgcgctcctg tagcaacaaa ccacacattg
     1561 tcggcgttta ccgaacagcg gttcagcaga atctgactgg cattgggagc aaattccgcc
     1621 ggccgggagg aaccctgatg gcgggccttc cggttaatcg aaaccttctc caaggtaatg
     1681 cggcacccgc tggctcccac gctgttcatc gtctcatcga taatcagatt tctcgcccag
     1741 cagtcctgcc cgctgatgcg cagggccgaa aagtgcggct gcgtatgact gatgggctgg
     1801 ggcggcgact gtatatgcag aaattccacc ccggcattgc tgacacgctg cggcggagta
     1861 attttcacaa ccttcacccc cggcggatta agccattttg aatcgtatga atcggacagc
     1921 ggcacatcca ctgttatcct gctgccccga atggctgcga tccgccgctc ggcatcgacc
     1981 gtcgagccaa cagccagcca tgtttgaggc cggccgtcgc gaacaaggtc atccatctgc
     2041 ataaacttaa cccaagcctc tgttaccggc tttcggatca gaatcgtatc cccaacagcc
     2101 agcccattga catcggccac agtgaaagac tgcgccccgg acggcacata ggcatcggta
     2161 atctccgtct gcactccctc cggcggctga atatctccgc gcggccgccg gcctgcggtg
     2221 cggcgaacag caacagcggt aaagggtctt cccgtcagct gcagcgttga gcaggccgga
     2281 ccctgttctc cgctgcctcg caaaacaacc ccgtcagcgg caatcgtaat cgttcgcgaa
     2341 atcgtaaacg tccccggagc caacagcaca gctccgcgga aaccgtcttc cagcggcatt
     2401 gccgaaaccg catcaacagc cgactgaatc gcagccgtat catcgccgcc ggtcggccgg
     2461 actgtctgct tgacaggcac tgtcggcaac ggcacaccgc cgcctttata gccggcatgt
     2521 gaaaaatcca ttatccggtc gcctgcaggt gtggttttat aaaccagttt cccatccggt
     2581 cccggataaa tccactcgct tacggctctc tgctctgccc aggcaggccg cacggccaac
     2641 acgccggtca aaagtgccag acacaatatc tgaaatactg cttttaacgt tctttttgtt
     2701 ttccggtttt gcttgggcat acacattctc caacaaaaga tttccggcag gttttatacc
     2761 ccccgttttt caagagctta aaagcagccg gcggcgctgt caatgttctt ttgtctgcag
     2821 ggcgatttta aaagctcact cgataattgt cagacccttt gcggctgcat accggacgac
     2881 cgattcaatt tcattgctcg aagggcggcg gttcaggcgg ggatgcgaac cggcttgaaa
     2941 gcagggacga tactggtcca tcacattgac agccgttgcg ggacttatct gttcagccaa
     3001 aaaatccaaa atctccatgc tgccggccag attattgggc aataccagat ggcgaaccag
     3061 taaaccccgt accgctaagc cattttcgat acataaatca ccggtctggc gatgcatttc
     3121 ttgaaccgca cagcggttcc gctgcggata atctgcggca tccgcaagct gccggctgac
     3181 agcactgtcc gaaaacttca tatccggcat atagatatcc accagacctt ccaaaagccg
     3241 cagtgtttca accgattcat acccgccgca attgtacaca atcggcagat gcagcccctg
     3301 ctcctttgcc agcacaatcg cctcggcaat cgggggaaca atatgggtag gggttaccag
     3361 attgatattg acgcagccgg aatcctgcag ctggagcatc atttgtgcca actgccggga
     3421 tgttgccggc accccgctgc gaaaatggct gatatcgaaa ttctgacaaa agaggcaccg
     3481 caaattgcac cccgcaaaga aaatagtgcc cgaccccccg ctgccgacaa gaacccgctc
     3541 ttccccaaag tgcgggccgg ccgaactgac cacggccatc ccgccaatgc cgcaaaatcc
     3601 ggtttgcccc tcactgcggc agaccccgca ctgccgggga caaagcctgc agggattcat
     3661 cagcgatttg agccggccga ttgtcttctg cacagcgttt tccatccgca gtcctcacga
     3721 gacaaccgga agctggtccc atcgggtcgt atatttggga gagagccggt tgaattgcgt
     3781 gcgccacggt ttttcaagac cttcagcccc ccagcagatc gaacagccgc ttctggcatt
     3841 gatggcatct atcgcccgca tcagccgcct tgacttgtcc cggtctgccg catcaaacag
     3901 gcacttctgc acttttgcct catcccccaa atgatgcaga agcacacccg ctttcttgta
     3961 ttggtaccca gaccggtaga ttttcccgag agcatccagc gatgcccgta tgagttcgac
     4021 gctgtcgctg gtctggacag gaaattcaat ggtgcaggcg ttaaaataac gtttctgtat
     4081 aaaccggctg gtggcggcaa aaacggtcag cacgccggcg gccagatgcg cttggcggag
     4141 tttttcgccc gcccgcgagg cataggaggc gactgcttcg ctgagctggt caaacgattc
     4201 gacaggtctg ccgaacatcc gcgaaaccgc cagcgacttt ttcgaaggcg gattttcttg
     4261 cagcgggtaa caggcgatgc cccgcagctc atagaccgtt cggacgcctt cgatgccgaa
     4321 ctgctgcctt atccattcga tatccgcacg ggataaatcc aaagcagtca atatccctgc
     4381 cttgccgagt ttgccggcgg tgcggtaccc tactccccac acatcggccg cagccgcccg
     4441 ccgcagcgcc tcctgaagat agggcgaatc tatcaaattc agcacgccgg cggctttgga
     4501 agatttcttg gccagcttgt tggccagttt gcaaagggtc ttggtggaag caattcccac
     4561 cgatacagga atgcccgtcc actgtctgac ggtctgctga atccgccggc cgtacgcagt
     4621 cagagaaccg gctatgcccg acaaatccag aaacgcctca tcaatcgaat aaatctctat
     4681 attcggcgta aactcagaaa gcgtctgcat cacccggctg gacatatctg catacagagt
     4741 gtaattggaa gacagcatct ccacatggtg tttgtccaga atatcgcgga cctcaaaggc
     4801 ggccttgccc atcggaattc ccagcgcctt cacttcgtcg gaacgggaca caatgcagcc
     4861 gtcattattg gataatacaa caacaggtct gttctccaga cgcggattga aaacccgctc
     4921 gcaggagaca taaaagttat tgcaatccac caatgcaaaa atattgctca taccggatga
     4981 atcacactgg taaccacgcc ccaaacctga aaatctgtct ggtcgctgat ttgccggggg
     5041 gcatattttt catttgccgc ctccagaaaa accttctttt tgcggatgcg gatgcgtttg
     5101 accgtcagtt ctccattcac agcggcaatg acaacatttt tatccttcgg ctccagcgaa
     5161 cggtccacaa ccagcaggtc gccgtcgtgg atgccggcac cggtcatcga atcgccgcta
     5221 acccgcacaa aaaacgtcgc tgccggattt ttaatcagat gcttattgag atcgagctga
     5281 ttttcctcgt agtcggccgc cggcgaggga aaacccgccg gaaccttggc ctcaaaatac
     5341 ggctgtctgt atgcagtcga tatatcggat cgataaatag ctgacacttt tgctttcata
     5401 tctcaattat accacagaat acgactgtca aaaatgttct tttgccccct cttttgcaag
     5461 cggtttgagc cattgaagtt atctgttaaa aaagcgggcg gttgagggcg attttttcca
     5521 gctgcggata acgcagcatc aggtcttcgg ggacaagaac tttccggccg gtcagtgcgg
     5581 cctttaattc caacgcattg gcaacctcag aaccccgata atgattattc gtaatcacca
     5641 caaccgatgg gtaaagtgcc atcagctttc ggatgcggtc ttgaatctcg gccagttcct
     5701 gcgtactgta ataataatca taggtttcat cgcgtgtgga tttggaaaac cacttttcag
     5761 cattgcgccc gtgcagccgg aaatagccgg ttgtcccgac cgcacaggtc tgcagattga
     5821 aggaatccct gccaaccgga taatcgatgt tggcaacaat gacacccaag tcttttaaaa
     5881 agtttaaagc atccgccttt tcccacgaac ggtgccgtac ttccacaacg atgctgaact
     5941 gcccaccgaa ctgttcagcc agcttctgta aatggtcccg atgcccgtcc ctgtcgtcaa
     6001 aatcataacg aaattggatg agcagatggc gcagcttgcc ggcctccagc agcggcgcca
     6061 gtccgcgatg aaactgcctg accatgtcgg gttcaatcct gccttcgtgg gaaaaatccc
     6121 tatgcagctt ggccgtgaag aaaaaatcgg gtttagtttg cgtgcgctgc agccaccccc
     6181 ggcaggattt ttcttccggc tgacgataaa aggtgctgtt gatttcaatg cagtccacat
     6241 acctgcacag atagcccagc ggatccttaa gaccggccgg ataaacaatc ccctgccagt
     6301 ccggataaga ccatcctgca accccgatgg aaacacgctg agtcatttgc aagccgcctg
     6361 aaaaaagtct ctttcccgtg ctcaaattct gactggtctt cggccaatcg gttcaaaacc
     6421 atccgaccgt actcatataa agtacgctct cttctgtgcc gtccgcaccg attatctgat
     6481 tgacttcatc atccgccagc gccccgatgg ggcagctgcc caaacccaaa ctgacagccg
     6541 ccaaagccag attttgggcg atatgtcccg catccaaata aatataccga taggcccgct
     6601 ggccgtattt ctgcttggtc cgctcaaata ccgctgtcca gataaaaaca gccgacgccg
     6661 aagcacacat cccctgcccc agagcggccc gggccagacg gccgccgtaa tcgccttctc
     6721 ccagcagttc gagagaatga ttgcggatgc tgtaatggta gaggccggcc ggaatatccg
     6781 caaccttgtt ggcggccaga taggtttcta ttggatacag tgcccccgct gaaggggctg
     6841 tccgaaattc atatcccccc tcgacgcggc ttatcccggt gccggcccac agcagatagg
     6901 aaagcatttc tgcagaaatc ggccgattct ggtatcggcg gatgcttctt ctgtttttca
     6961 gcgcctccga taatggcatg gcagaaagag cacgcacatc gggcagcttt atctgcggac
     7021 tgtcggggta ggttttatac agcttcggct ttacagcccc tgctgaagaa ccggcaggca
     7081 ttgtatccgg gcggtatttg gtttccttct gaaatctgtc gcctgcatca tgcatttgaa
     7141 ttctccgccg aataaaaatc aactcccccc cgtttccctg accacaggat aaagacccag
     7201 cagcacagag gccttacgct gccgcaagag gcgtctttgt ctgccggcgg gctgtcagcc
     7261 agcttactac aaccagcacc gtcaccgaca cagtaatcgc cggcagaacc tcatccagca
     7321 tagtataata actgccaagc agcgctgcca taaaggtcat ttttctccac agcagcgtca
     7381 tggcggttcc gcaaaggatg gaagcaacag cgccgtgccg ggtagcgcct ttccaaaaca
     7441 aggccgccac caatgccggc gtaatagagc ttccgtaaat ggtataggca tacaaagccc
     7501 tcgataaaaa tcccggcacc cggtcaaatg tcatgctgat caaaaaggcc gctgccccca
     7561 gacacagcac catcagccgg ctcagaaaaa gcgtctgttt ttctgttgca gaaggattca
     7621 gataggcctt gtaaatgtcc cgaatcaacg aggccgaagg aaccagcagg aatgaatccg
     7681 ccgtggacat aatgatcgcc acaatattgg tcataaaaat gcagcccagc catggaggca
     7741 tcaggtagcg ggaggccgca atcagcacat atttgccttc ttcggcatcg ggaataagag
     7801 ccccggccgt ccagccgcag ccgataatca gaatctctat cagcagcgcc gcaaagatga
     7861 gaaccagcgt cgcctgtttg gcgcctgcgg cgcttttgct ggagaaaaac cgctggtaga
     7921 gattggcatc gcccaaaacc agcagaaagg ccggcagaca caggttgatg acagaaaaac
     7981 catccagatg cgcccagaag cgcatattct cgccccgtcc catggcggcc agcgcggtgc
     8041 ttatcccttc ccatccgccc gctttgttcc acagataagc aaatgcagca agaagcgagc
     8101 caataatcaa aatgccgttg ccgatatcga tataagcaat gctgaccata ccggcaatca
     8161 tcgtaaatgc aataataaag gccgctgcaa tcagggttgc ctgacggatg gacagcttgt
     8221 actcctgcag aaacaatgtg ccgcctgaaa tcgaagcaac agaatagcgg ccctcggccg
     8281 tttgcacagg acgcagaaca actttctgcc ccggcgccgc agcaatccag cctgcaagac
     8341 cctgctggtg gatttcaata ttggcctcct gcaccttttc ggctggtaca acacgcaggg
     8401 caacagtaat cgtatctcca gactccggcc ctgccggccg caggcatatt tccggccgcc
     8461 cagtaaaacc ttcggaagga acaaaataaa catacccctt gcggagctga tacgcggtaa
     8521 ctgtctgatt ttcggacagc acggtcggtt cttttttgcc gactgcatac tgcaggacac
     8581 ccccgccggc attaaactga taactgacaa taaccagata cgccgcaacc agtgccagaa
     8641 ccgccatgac ccgagcggta aagccgaaac gcgcttcaat gatttccggc acactgaatg
     8701 tatcgaaatt gcggactttg ggcgcaataa agaccagcag aatcatcccc agcgccggac
     8761 ccaccggcag aatcatggcc cccaaaccaa tagcgtaggc cttgccggca ttacccagaa
     8821 tggaacctgt acccacccat gtggccagca tcgttaagac aagaatccaa accgacagcg
     8881 tacggccggc cacactgaag tccgcttggg tcttgacttt tcgcacctga cgcagagcaa
     8941 caaacagcag ccccgccaga tacaccaaaa tggcggttat atatatcata cgatactccg
     9001 gttaacctat cgaagccgcc agtccacccg ttcaggatgc ttaaagaggc cgctgatgaa
     9061 tgcttcaagc cgggggtcat gctcctgaag atgctggcgt gtgccgagcg gctgtccgtc
     9121 agccgctgtc agagaaccac cgtcaaaaaa ggcctgcacc cccgccgcaa aatagtcaaa
     9181 cgcattctgt gccgcagccg ttcctttcca aagtccctgc tccatagccg tttgatagag
     9241 ctgccggacc ttctcatatg tccgccggtc aagacgctcc aaatccttct catactgctg
     9301 cttgagccgc cggctgtcat aggccggatc aacttcccga aaaccggtat ataaaagcaa
     9361 tgccttggcc atcgcaccaa tcagcatgct tgcctcattc ggcctgctgt tgaggccgtc
     9421 cagcagattg gactggggta caccgaaacg cagccgcttg ggaagcgtga cggcaggcct
     9481 gtttggatcc tgagcatcca caaaagccgc cggattcagc gctgtcaggt ttgccggctt
     9541 ctgaccatcc tcgtaaacag ccaattccag cccgccgtca atgagggcct tcagaacatc
     9601 gtggcggtag cgaaacatat tgcggacgat gcggtcggct tccagaagcg ccgcctcgga
     9661 aacgccggcc gtgccgaaaa tcgaaaagcc gcggcaccag acataatgcc taaacacagg
     9721 gtctgtttcc agatcggacg gaactggaat caccgacggc tgtttcttaa gagggacata
     9781 agaccatgcg ttttcatccg taaatcgaaa gatttcggcg ataaaatcct tcatttccgg
     9841 gtcataggcg gccagctctt ggcgggtatt tatctcgttg tgattccagt tggtctgccg
     9901 gtcgcagtta aaatacgcct gaacattttc ggcccagtac tccgacggat tggtcgaggc
     9961 ataatcatac ttgtacaagc ccttgtccat cgctttccga taaagggctt ccagacgggg
    10021 ctgaaacgtc ggatcaatcc tctgcaaagc cggggaatga atcgcatggg caagctcatg
    10081 aacaaagata ctctcatctt cataacggtc catcggcata ttcagaagat tttcctcccc
    10141 gcagcttgtg gtcagacccc caaatcctct ggcccgctcg ttccagtact ctttgggctg
    10201 catatgggag tacatcggta tatcggtcac ctgctcatca tgggctacga tcacatactt
    10261 gacaccggca tcggccagcg cttggtgaat gtccggccgg ttggcaagaa tcgaattcag
    10321 aagttcgcgg acccggtaaa aagccgcatc cgctacacgg ctggaaccaa taactggaat
    10381 gccgccgcaa tcaagatatt ttgaaaaaaa ggggtcataa ccagaaggag gagtggtaat
    10441 ttcattttct gccgaaggct tgaccgctga gtccgatgcc gaagagataa ttgaaaacga
    10501 taaaaccgct gatatgagga aaagagctgc ctttttcatt tctgactcct gccgtcttta
    10561 tttcgccgat actcccatag aaaaaccgcc gacagtgcca ttttacatcc aattcttgac
    10621 ctttcaagaa ttgttttggt tttccgcagt ttcagacaga atggagcaca aacggcaggc
    10681 agaccgttgc atcattcagg accatgcaaa accgaaattg aataaaaaaa ccgcctgcgg
    10741 gcagaaggag gaagcagcgt gcgctgctta gggctatccc acaggcggca gaaggagctt
    10801 atcttcatcg tatcaaaata atcaatttat gtcaatagca attaccgaag tttttcaaag
    10861 aaagtccagc ccccgcctgt gctgtccctt ttttttgaag ataatggatg gactatctaa
    10921 atgatttgcc aacgtccccg gcgggattcg aacctgcaac ctttggctcc ggaggccaac
    10981 gcgctatccg ttgcgctacg gggacctgtg aacgatacta tacagcccta tctatttcct
    11041 gtcaattcaa tatcaaaaca tccagaaaaa tagcggtttg ctaaatgcaa aatgcggaaa
    11101 tagggatgaa aaacggggaa taaccggaca gttgcctaca tctgccagtt caagggatag
    11161 cggacacgct gaggcgagat attaaatgta tggactaaac tttcaaccag cgtaatagtc
    11221 cgcttgattt ggaaatccgt tactgaattg ctgctgccgt cgccaatcac acagatccga
    11281 accacaccgg ggcgtccgtg acataaccgc tgcagcttcc agagatcact ggcctgaata
    11341 tgcccgtcgc cggctccatt gccgttgccg actacaaaat gaaactcagg actgctgccg
    11401 gtcgtcaagc ccatcatcag gcccagttca tcggcattgc cgcccgaagt acggctgtaa
    11461 aaaatctcca cctcctgcca ttggcctgcg gggacagaca tcgtatttcg aacagcatcc
    11521 tccacagggt ctaaacgcag atagctggac agactgtacg cgccggtcat aggacggtgg
    11581 ttatccagcg ccatcaatac cgccgcaccc actgtcatcg atgctattaa acaaaacaat
    11641 acggttacat ttctatttcg cttggccatc cctgtgtctc gaaattcgat acgtctgatc
    11701 aattccgtgt attgctcggc taactgaatt atactatgta cttcggtagt tcgcatcatt
    11761 tttatacaga aattttacgg atttttaatg ctcccattaa attgtatcaa atacatcggc
    11821 cggccatctg ctcgaatttg aagagggatt tgaaatccct tttctttttt tcaaaaaaaa
    11881 ctataatgaa tgacgattat aacggcagaa aggtatatac tggaaatgcc gtgtatccgg
    11941 ttagcggcaa tccatgcgga gtattgaacg caagataagg agtatttatg tcggaattag
    12001 atgtcattcg tcaaatcgcc catcaggcac tgacgatacc gactctgaca ggaatcagcg
    12061 acaactggtt atgggaccgc acgctccgta ttcttcgaac ggttgaacat atctgccggc
    12121 tgcctgaact ggtcaatcgg gctgcggctg tggaccgttt ctgcctgatt tcggccacgt
    12181 attttgcaga tgccggcttt gcccattata tcgatgtgga aaatgtgtct gcccgtctgg
    12241 tgctggcgga tatttctctg gccgaattgt gtgatttttc aacacaggtt gtgtccgacc
    12301 gtctggtcgg cgcaatgtcg gggcttcgca tcgataagat taaccgcatc attctcgaat
    12361 cctgcaaccg ccagacgtca atgattgagg cgatgattct gtcggatgca cgcaatctgg
    12421 aagatatggg agctgtcggc ctgtttcacg agcttcgccg gtatgtgatt cacggcaaag
    12481 gcatctcgga aattcttgac agctggaaac gcaagattga ttacggctac tggcaggccc
    12541 gcctgaaaga aagcttccgg tttgaaccgg tccgtcttct ggccgaacag cggtttgccg
    12601 cggccgagta ttttatgacc cagctggcta cggaaaatgc cgcacgcgac ttagaggaac
    12661 ggcttttgga atcgctggat ttcagcaaat cggcggattc cgcagaaccc gtcgcaagcc
    12721 agcccggtta acaaaattat gaaaattgaa cccatcacag ccattttccc cgggtctttc
    12781 gatcccatca ccaatgggca tctggatgtt atccggcggg gcaataagct gtttgaccgg
    12841 ctcattgttg ccgtcgggca gaatcccggt aagcgggagc tgttcagcaa atccgaacgg
    12901 gtcgatatga ttcgccagct ggttgcagag atgccccgtg tctctgtgga aagctacgac
    12961 tgcctgaccg tggaattcgc caagcagaag aacgcacagg tgatgctccg gggcttgcgc
    13021 aacctgacgg atgtagaata cgaattccag ctggctatga caaatcggac cgttgccggc
    13081 attgaaaccg tttttgttat gaccagtgaa gaatacggct atgtcaactc cacaatggtg
    13141 cggcagttag ccatcctcgg cggggatgtc tcaaaattga ttcccgctgc cgtttacgag
    13201 cggctccggc aaaaaataca gaaaaatccg catccggcca atccagacgt gccggaatag
    13261 ccaaaaatga ccgcttgaca tccaaaaaaa ggataacaat gtatacaatt tttgttgaaa
    13321 tgccgttcaa ggcccagcat cagctgacct tcgaggatgg cacacaggag ccgctgcacg
    13381 aacacgaatg gaaggtatgt gcggctgtgt cggcggaaaa attgaacaaa gaagaaatgg
    13441 ttatagattt tgaagagctg aaatctcttt tagataatat cctgcagaat tttcagggcc
    13501 agcagcttga aacattgggg atttttgagc accggaatgt atccgctgaa aatcttgcct
    13561 gcacgattta taagcagctt gccccccaat tgcccaatac tgtgcggctg aggtatattg
    13621 aagtaacaga agcccccggc tgccgggcac gctataggcc ctgattgccg ctggtatttt
    13681 tttcactcga aaaaaacaag caaagccggt ataatcagcg tagaggtaaa tggaaccgca
    13741 tacaaaaggc aaaataatgt atgatttgtc aatcctgtaa agaaaaaacg gctacgattc
    13801 atctgaccga aattaccaac gggcaccggt cggaaacgca tttatgtcag gattgtgccc
    13861 gtcagcaggg tctgaccatt aagacccaaa taccgattaa tgagcttctc aataccctgc
    13921 tgagcgctca gtccaagtcg caaagcaccg gctccgtttc ggtgcagtcg gggcgggcct
    13981 gtccggtttg cggaatgacg ctgaagcggt ttgccgcaga accgctgctg ggctgtcctt
    14041 atgattatac ggaatttcag gccgagctgc tgccgctgat tgagcagagc cacaacggca
    14101 aaagccgcca ctgcggcaag acgcctgcac gcaccagcca gcaggatcgc aacgaaatta
    14161 tgctcattca gctgcggcgg cagcttgaac aggccgttaa aaatgaagat tacgaaactg
    14221 ctgccaaact tcgagaccag ataaagatgt accaatgaga cccgcagaat tgtgcaaata
    14281 tcccagttca tggtttggag gcggctcgga tgttgtttcc gaagtcgtca tctcctcgcg
    14341 gatccggctg gcccgcaata tcgccggcta tgagtttttg ccctgtctgt ccccggcgcg
    14401 gcagcggcag ctggaagacc gtctcaggga aatactcctg tccattgatt tgggcaaaaa
    14461 agtgtttttt atcgatattg atcaggcctc tgatgtggag cgcacgctgc tgaccgaacg
    14521 gtatcttatc agccgccgcc acgctctcgg caaaggcccc cgcggcgtgg tcattgccga
    14581 agatgaggca ttcaccgcca tgattcacga agaagaccat ttgcggatac aggtctttgc
    14641 cgccggcctt cagctcaacg cctgctggga tcgaatcaac cgcatcgatg acgccatcga
    14701 gcagaaaatc gactacgcct ttgacccgcg gctgggctat ctgaccgcct gtccgaccaa
    14761 tctgggaacg ggcatccgcg tttcggtcat gcttcatctg ccgggcctga aaatgaccgg
    14821 acagatggaa aaattcatca ccgcagcccg cgactgcgat atggccgtgc gcggcctgtt
    14881 tggagaaggc accgaagcga ccggcgattt ctttcaattg tccaatcagg ttacgctggg
    14941 catcagcgaa aagcaggttg tcgaggattt caccacccat atcgtgcccc gcattattgc
    15001 ctacgaaacc cagtgccggc aagccctttt ggagcagcgg ccgcagctgc tggatgataa
    15061 aatctaccgg gctctgggag ttctccgcag cgcttgtctg ataagctcgc aggaagccct
    15121 tttcctgctc agcaatgttc gtctgggagt caatctggga cgcattaaaa acattgatat
    15181 tgccaccatc aatgaactgt ttatgctcac ccagccggca cacctgcaga tacgggccgg
    15241 ccgaatcctc gatccggacc agcgggacac ccttcgagcc gaaatcatcc gtgccaaact
    15301 caaccagaac taagaagctc cccatgagaa caaaacacgc tctccaattc tgccggttac
    15361 ttggactgct tggggcggta ctgaccgccg gcatttcctg tgtcagcccg cagcatgcat
    15421 ccgcacccgg cagagccgaa ccgctggaca ttctggtgct gaccggcgga cacggctttg
    15481 agcagcagcc cttttatgaa atgttcagcg caatggaagg ccttcgcact gcctttgcag
    15541 aattggacca gcagagccgg tattgggaga ccctgcaagc cggcgatgcc gatgtcattg
    15601 taatgtacca tatgcggcag cagattaccg acaaggccaa ggcaaacctg ctggcgctgt
    15661 gcgaaaaagg aaccggcttt gtggttctgc accacagcat ctgtgcttac aatgactggg
    15721 atgcataccg aaagctcgtc ggagccgcct atttgcacca gccccgcacc atcgacggta
    15781 cggttgtgga gccggggaca tacaagcacg atgttgctgt gccggttcat atcgccgcca
    15841 gccatccggt cacggccgga cttgccgatt ttgagctgtt cgatgaaggc tacaagggct
    15901 gctggttcga accggacaat accgtcctgc tgaccaccac ccagccggcc agcgacccgg
    15961 ttatcggctg ggtccgcaca ttccgaaaca gccgggtttg cacccttcag tccggccacg
    16021 ggaaagatac ctttgacaat cccgcctacc gccggcttgt gcagcaggcg attgtctggg
    16081 cggccggccg gtaatttcac cgcattgcca aaaaacgccc tgaccggcaa ggtctgccat
    16141 tatggcataa atgaccttct ctgccgaaaa ttacaacatc tttctccaca aggagttaag
    16201 ctcaccaaga agccgctggc acagactttg cacatcatcc gacataccgt atttataaaa
    16261 tgccgtaaca ataccaaatt ttacctgttt ggaaaaacat aggagaacag caaatggcta
    16321 aaatcattgg aatcgacttg ggtacaacca actcggtagt agcggtaatg gaaggttcat
    16381 caccgaaggt gctgatcaac gcatcgggtt cccggatgac cccatcggtt gttggtttta
    16441 ccgacaaagg cgagcggctg gtcggccaga cagccaagca ccagcaggtt accaacccca
    16501 agaataccgt atattcgatc aagcggttca tgggccgacg ccataaggaa gtcagcagcg
    16561 aagaaaaaat ggtgccctat accattaccg gcgggccgga tgagttggtc aaggtcagta
    16621 tccggggcaa agaatataca ccgccggaaa tttcggcaat gattctgcag gacctcaaaa
    16681 agaccgccga agattatctg ggcgaaaaag tcgaacaggc cgtcatcacc gtgccggcct
    16741 acttcaatga ctcccagcgg caggccacca aggatgccgg caccattgcc ggactgaagg
    16801 tcgagcgcat catcaatgag ccgaccgccg cggctttggc ttacggtctg gaaaagaaga
    16861 aaaacgaaaa agtcgccgtc tttgacttcg gcggcggaac ttttgatatt tccatcctcg
    16921 acatcggcga taatgtgttt gaagtgctca gcaccaacgg cgatacgcat ctgggcggcg
    16981 atgatttgga tgagattctg attaattatc tggccgacga attcaagaaa accgaaggca
    17041 ttgacctgcg gcaggatccg atggcccatc agcggctcaa agaagcagcc gaaaaggcca
    17101 aatgcgaatt gagcacccag ctggaggcaa cggtgaatct gccgtttatc accgccgacg
    17161 ccaacgggcc caagcacctg aacatcacga ttacccgcag caagtttgag gccttggccg
    17221 agccggtctt tgagcggctg cggcagccct gcctgaaggc gattgaagac tccaaactca
    17281 aagtcagcga tatttccgaa gtgctgctgg tcggcggctc aacacgcatt ccgaaggtcc
    17341 agcagattgc acaggaaatc ttcggcaagg ttcccaacaa gagcatcaat cccgatgaag
    17401 tggttgcgat gggagcagcc attcaggggg cggttctggc cggcgatgcg ggcgtcaagg
    17461 acatcctgct gctggacgtt acccccctgt cgctgggcgt tgaaaccctc ggcggcgtga
    17521 tgaccaagct gattgaacgc aataccacca ttccgaccag caaaaaagag attttctcaa
    17581 cggccgctga cggtcaaacc agcgtggaca ttcacgtcct tcagggcgaa cgcgaattcg
    17641 cccgcgacaa ccgcacgctg gggcggttcc agctgtcgga tattccgccg gcgccgcgcg
    17701 gcgtgccgca gattgaggtc accttcgaca ttgacgccaa cggcatcctg
//
LOCUS       DAOTBO010000058        17376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2715_length_17376_cov_5.959702, whole genome shotgun sequence.
ACCESSION   DAOTBO010000058 DAOTBO010000000
VERSION     DAOTBO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 17376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17376
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2715_length_17376_cov_5.959702"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..743
                     /locus_tag="PLE88_07870"
     CDS             219..743
                     /locus_tag="PLE88_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76418.1"
                     /translation="MNEKALSYGDLVEQWKVELIVQRARHFGIPEHDWPDIQQSIILE
                     ILNFKYDPKNAQGAEERTALTTLIDNRIIDLLRRQTNQDERHEEYLRENGFTSPREES
                     LSYSQNNALHCDIKNAIANLSVKEKAVCAGLMEGLSIRHLAQKLRCRQQSIQYMIQNI
                     RRVFKELGVHKWLQ"
     gene            731..955
                     /locus_tag="PLE88_07875"
     CDS             731..955
                     /locus_tag="PLE88_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76419.1"
                     /translation="MAAVKSPRNHAKMTGFSANNSADRNTESPLGGGLSGQELADLTV
                     LWSRVYGRTLTEDEVLEIYRNVKHLAETLI"
     assembly_gap    1087..1186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1187..1722
                     /locus_tag="PLE88_07880"
     CDS             <1187..1722
                     /locus_tag="PLE88_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76420.1"
                     /translation="RWKEEDVEKAIFDELKQMVIPDRHFRESFRRTLEVALSDSSEYQ
                     RQQDRQMKKRLSEIQSMKDRLLTAYLGSLIDEMTLQNKTTELATEEKSLKMTLQMTPY
                     EPQSDRIKRILGIFEFSQNIEKYWRGSNSAEKRKLLEIVSLNRHLSDVSLCVEKRKPF
                     DFLAKRLVLEKSRGDWT"
     gene            complement(1704..1778)
                     /locus_tag="PLE88_07885"
     tRNA            complement(1704..1778)
                     /locus_tag="PLE88_07885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1741..1743),aa:Pro,seq:ggg)
     gene            1944..3074
                     /locus_tag="PLE88_07890"
     CDS             1944..3074
                     /locus_tag="PLE88_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOM76421.1"
                     /translation="MIYKTYGKTGLNVSAVGFGGMRFDTARSKEENAALLRYAVDKGI
                     NWLDTASGYCDDQSEDIFGLAIGQMASRRSAFYVSTKGMPENCPTAAKAQEAVEKSLK
                     RLRTDYIDFYYVWCIRRWKEYELATRPGGQYEGLLKCLQQGKIRHLVVSTHLRGPDVQ
                     RLLGKMEFEGVLLGVNILNFMYRWQGVQAASELGLGVAAMNPLAGGIIPRHHEQLGFL
                     AGPGETPTEAALRFCISCPQITVTLVGFTLREHIDTACRIVDSAELFSQEDIERIRRR
                     LTCSMDNLCTGCGYCMEYCPRNIPISNYMQYYNYKLLDKRSDDKMIELLKEQYEWGML
                     ADRPAGAEDCVRCGRCELACTQHLDIIRRLEEIAVWEKSCSP"
     gene            3366..5111
                     /locus_tag="PLE88_07895"
     CDS             3366..5111
                     /locus_tag="PLE88_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76422.1"
                     /translation="MQERNDTYITYRLLTGLGIILFAVAGCRDNPETKAAKAVRKQTA
                     EAVRVSATEKDYPAAQQKVMAALVQNRTGGLTQDAALLASGTIALANGRQMQSDLGLK
                     TLPLNQCADNIRKTLRRLEQLLLEKKRIEGLLAGGDLEVAELKKLLVGDAEHPGLQAQ
                     LEQANEQLSQLLSQKQKLQEEKNKVQAVLDEQQAQADALLRKAEMARGDEKLSLQQQA
                     YAVLLQRKDFYVQAQAAENAIKNLDADTALVQVRIDGIKQSIEQVGQKAAAIEASAAR
                     VSLKQQMADIEREIAVYQQSLTALADKLKNDFSAYQETADRTAAIFEEASAEFEKIHS
                     NDAQFSAAIGLAESAHRAALAGEAPVKIQLDLARRLQNMTETADPNFAGVLQSRLSLP
                     LQVQPEVKQKILALYDRAIENYEKALAQASRFGSEAECSLLKSLVIASHDKMKFADLC
                     QDFDLANAAETAMMALIQKGTEKGVCFTQSEVLKIIESEGLNYLPSMPLNMEVFIEGK
                     RQELSAWKRLPISQQEAAVNEALPQIDALITQYGQQMADALEPLKQEMLAAKERGFKE
                     PVPAAGGPGEPNSLF"
     gene            5233..8100
                     /locus_tag="PLE88_07900"
     CDS             5233..8100
                     /locus_tag="PLE88_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOM76423.1"
                     /translation="MKTAETIGAELAGLIKGTVRIDLCTRTAFSTDASIYHILPQCVV
                     SPRDEQDIIAVVSYAAANQIPVAPRGAGSGLAGESLTSGIVIDCRRFMNAIVDTAPDG
                     SWVRVQPGVVLDKLNAHLVSFGRKIGPDPSSSNRAVMGGVVANNATGAHSLQYGYIAD
                     YVDSVRVVLADGTPVTLSSCICPESAPAPVAKLASACAEVLGPSRELIDKAQPATKRN
                     RCGYTLVNVVQNGRLNLARLMVGSEGTLGVFTEVTLRTVPVPAAVGLIQFEFASMEAM
                     ARAVPIIVRSGAAACELMDRTLMDMARQALPRYRDVLPAGCAAALLVEHVGDAAGEVS
                     AKIEHTISAVGTLAADAMKILEPDRQAFLWKSRKDAVPLLNRQKGPSHPVAFIEDVSV
                     DYHHLEKYILGLQEIGRRYDIPMAFYGHAGDGELHIRPYLDLSRPEEIERMRRIAEEV
                     FALAWSLGGSISGEHGDGLLRAAFLEGQYGKDYCQLLRQIKRIFDPAGLMNPGKILSD
                     DPDVMIKNLRAPALKAAEDFQTQLFFKPQEYRYEVEQCNGCGVCLARTAGSRMCPVFR
                     GLDDELASSRAKANLIAAWMARQPLEAAALKDMLSLCINCKMCSIDCPAGVDVSKLVI
                     EARTQLARQTGFSAAETVLSNNRRLSMLASAFSPLSNRVLSLGGVRWLLEKLIGLDRN
                     RRFPHFEHGSFIRKARRHLQKQPPLAEPIDKAVYFVDSFANWNDHELGFAVLDVLNKL
                     GIQTAVPPQRPAPLPAYVYGNLKTARKDLEYNVRQLLPYVQQGYKIVCSEPSAALCLK
                     EEMRLILNTEAAAQIARNTVELMSYIEDIWCRNKRAVLENKNISGWAGKKFAYHAPCH
                     LKALKSTSAALKLLNKFGVNAVDLNAGCCGLAGTAGMQKKHRDLSISVGRPLAEAISA
                     AGADIILTECAACGMQIEHLSGKPAMHPIKILCRIWQENAC"
     gene            8165..8974
                     /locus_tag="PLE88_07905"
     CDS             8165..8974
                     /locus_tag="PLE88_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM76424.1"
                     /translation="MKLTDVLRYECIQAGSAVDDKAMALCEIASLAKKCRLLKSVSEE
                     TILEALQERETLGTTAFGRGIAIPHCRLPGLRDFVVGVLTSPQGIEFESEDGQKVHLI
                     VFIIAPHEQSDRHIRLLSMVSRMLQDDSVVEKLVEAKSADEVKAVLFESARDDLAEQL
                     PVVRCQIHIFVQNEKIFRSILEKLAGLDDILLTVMDAKPTRSYLSKTSLYADFSKNGK
                     AVCKVVVAVVEHSLTNEVIRRVETITGPLLECTGVMVTVQELSYFAGSLEM"
     gene            9105..10061
                     /locus_tag="PLE88_07910"
     CDS             9105..10061
                     /locus_tag="PLE88_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM76425.1"
                     /translation="MADKRIQIYHNLSVMLNAGVPIVRALRLGHSTGKYGRLFSCIEQ
                     DVAGGTALADAFEKHNKDFAPLDIALIRVGDETGQTAEMFEALSQWYAFRQRMINTVR
                     FGLLLPVFYIHAAAVLIPFVSFALSGWDWSIFFEGMFAILALAYVPALTILAVFYLTP
                     RQGLLRRILDQIILIIPLVGKAVRELSLSRYCNIFAITYKAGLPILQCAKMAADSIGN
                     SVLRSRLTGAYENVKAGREMSDGFSRYLPAEFINIWQVGEETGQLDDSAYRLGNMHAE
                     NAQRHFTMLAKIVPFVIYLIVIFLIAFFIIMAYSKLYGTLTA"
     gene            10226..10450
                     /locus_tag="PLE88_07915"
     CDS             10226..10450
                     /locus_tag="PLE88_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOM76426.1"
                     /translation="MRPRSTVVSTGTVKWFNASKGFGFIKPDDGGDDLFVHFSEIRMQ
                     GYASLDENQKVSFEVGQGKKGPCATNVVPA"
     gene            10587..11000
                     /gene="rnpA"
                     /locus_tag="PLE88_07920"
     CDS             10587..11000
                     /gene="rnpA"
                     /locus_tag="PLE88_07920"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOM76427.1"
                     /translation="MERLFFRKSQRVVSEREFAKVLSQKCFVCSQMLRLYAAPNNLNI
                     PRFGVSVGRAIGNAVLRNRLKRLAREVFRLNQHQIPAGYDYILIFTQKMPKQKAGTAK
                     PKKQELSQMQVEDLEHLFLSMLHNLIKKSNKAENK"
     gene            11004..11294
                     /locus_tag="PLE88_07925"
     CDS             11004..11294
                     /locus_tag="PLE88_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOM76428.1"
                     /translation="MNTIAESQTSHNEQPKLMQIGPAARKAGVSRQSLQYYLMVGLLE
                     PTEISPTGRRLFDQQAIERIRLIHKLNKSGYPLRAIRELFVKSQQDRKGLEK"
     gene            11291..12202
                     /locus_tag="PLE88_07930"
     CDS             11291..12202
                     /locus_tag="PLE88_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOM76429.1"
                     /translation="MTDYQTRQRQKNMVVGVFVLIALIAFLWMLFRFRDLPLAVSKIK
                     SFVVLVYFPEAPGVQKDTPVNYCGYQIGRVMNVAPPQVYEVNGLKFHRVGVTMAIENR
                     YTDIPKNVDIYVMKRGLGSSYIELVQDPQKTALAEGFLEDKMVLENGKVGTTSDFFPP
                     QIQAKLENLVDSIAALADNANQIIGDQDNQTNIKKMLANIEAATAQADTTLKSIQQFS
                     DTGNEKVRQISDKIGLAAEQLEGALSEFRQVMAKIESGQGTAGKLLNDGRLYENLIES
                     SRELQMALQQIKEWAADAREKGIRIKW"
     gene            12785..13621
                     /gene="rpsB"
                     /locus_tag="PLE88_07935"
     CDS             12785..13621
                     /gene="rpsB"
                     /locus_tag="PLE88_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOM76430.1"
                     /translation="MNKELARELISAGVHFGHAVSRWDPKMKPYIFGKRGMIHIINVK
                     ETLKGLMIAKKLLANVVSNGRDVVFVGTKRQAKKAVQSAAEKCGMHYVSERWLGGTLT
                     NFRTIRSRLQRLEELEAMEASGQIESQSKKQGARLKRELAKIKTNLEGIRRMSRMPGA
                     MVVVDAKKEYIALREARKLGIPTIAIIDTDSDPDTVDVAIPGNDDSIKAIEILLDQMA
                     SAVAEGKTMVKQEDVTAAAGARAARTRSRRRVLAAAQETQEQTEEKSSTEVSQPAESP
                     AQ"
     gene            13737..14597
                     /gene="tsf"
                     /locus_tag="PLE88_07940"
     CDS             13737..14597
                     /gene="tsf"
                     /locus_tag="PLE88_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOM76431.1"
                     /translation="MAEISAASVVKLRRMSGQGMMDCKKALEEANGDLDKAMELLRKK
                     GLATLEKRAGRETTEGRVLCSTRNDGKTAVMVSLCCETDFVAKSDDFIQAAEALLECG
                     FAAAAEQGAAALMEVAVGGRKFADILTDTVSKTGEKTEVGDFARYTAGPNGIIGTYVH
                     FNHKVGAMAEIEADSPTVARALKTIADEICMHITAMNPMALDKDSFDPAVIERERAIA
                     AEQVKGKPANIIDKIVDGKIAKFLKEHCLIDQSFVKDDSKTVAQVLTEAAKAAGGRAK
                     IKRFVRIEIG"
     gene            14678..15403
                     /gene="pyrH"
                     /locus_tag="PLE88_07945"
     CDS             14678..15403
                     /gene="pyrH"
                     /locus_tag="PLE88_07945"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOM76432.1"
                     /translation="MTAVKYKRVLLKLSGESFCKPGGFGVEGEALESIAQRIARICRL
                     GPQVAIVVGGGNFLRGAAFSEKSHIPRTTADYMGMLATVINACALQETLEKMGQPTRV
                     CSAIEISAICEPFIRRQAIAHLENGRVVILAGGTGNPFFSTDTCAALRASELNADIMI
                     KATKVDGVYTDDPMKNPKAHLVESLSYQQVLEQNLRIMDPAAVSLCRENHIPVVVLNI
                     FKEGALTAALEGKKVGTLISDVA"
     gene            15442..16002
                     /gene="frr"
                     /locus_tag="PLE88_07950"
     CDS             15442..16002
                     /gene="frr"
                     /locus_tag="PLE88_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOM76433.1"
                     /translation="MPTRKIIAMHESAMEKTADFLRNELKSVRTGRASPGLVENLSVD
                     YYGSPTPLKSLATIAVPEPSSIVIKPFDPACLKDIEKAIKTSDLSLAPVLDGKMIRLN
                     IPPLSEERRKQIVQQVKQMGEKTKVSIRNIRRDANKQLDDEQKNKTITEDDCKKAKET
                     IDTLTKKFIDEVDSIIKHKSEEIMTN"
     gene            16020..>17376
                     /locus_tag="PLE88_07955"
     CDS             16020..>17376
                     /locus_tag="PLE88_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76434.1"
                     /translation="MSGQTSSAEKPKPSNRRVWFIVWGLVSIAGLAVLILFGIPLFLS
                     SAGGTQFLLSKINQSVDGKVQMDDFSIGWFKGIHLTNLSYADNAGMASVKVRRLETQP
                     QYWALLGGKINLGKTLIEGPDVYVKIPAVQEPAGKTQKRESGQQSATPSAFPIHQINL
                     ELVDGRATVELGGDVPQAVSFTNIASVVNLAAAGQRSMLDVSMNVVGQGEPAALSAKG
                     QATTDKTGWKLKEGAFDVKIDKLQLASLKPLFALAGKTVDIAGVLNAEGTIAIQNNAV
                     GQAFLTATIDNFSQGTGQQQIKTAKPITVTVSAAQQDSGIKIDKFAVASEFFNVNCSG
                     TAEAIDYAVDADLAQTQNLIAQFADMGGLSMRGMLDLRGKVLMKEQEIKAAGKGTVRE
                     LIVRKGDVETPKTNVDALEFDCTVDQAKNQLRVAMANMTAPPVGAVKISNVVVPMGEA
                     GE"
BASE COUNT         4592 a         3933 c         4655 g         4096 t
ORIGIN      
        1 gggtcatttt tagtcacgtg accgtcacgt gactaacacg tgacacattc tgaccccata
       61 aattagtcac gtgactgtca cgtgaggaca catgtgacta tcttgagtca cgtaactggc
      121 tacataaatg accccataac gtgaagatag tggtggctgc gatgaccggc attgggctgg
      181 tcaggaaaca aaaactttcg atacgaaagg acgcagccat gaatgaaaaa gcattaagtt
      241 acggggattt agtagagcag tggaaggtgg agttaatcgt gcagcgggcc cggcatttcg
      301 ggataccgga acatgactgg ccggatatcc agcaaagcat tattttggaa atcctgaatt
      361 ttaaatatga ccctaaaaat gcacagggag ccgaggaacg gacagcactt acaacattaa
      421 ttgacaaccg gattattgat ctgcttcgca ggcaaacaaa ccaagatgag cgtcacgagg
      481 aatatcttcg agaaaatggc tttacgagtc cgcgagaaga atcattgtca tactcccaaa
      541 ataacgctct tcattgtgac attaagaatg caattgcaaa tctgtcggtg aaggaaaagg
      601 ccgtttgtgc cggattgatg gaaggcctat ctattcgcca tcttgcacag aaattacgct
      661 gtcgtcagca atccattcag tacatgatcc aaaatatccg ccgtgttttc aaggaattgg
      721 gggtacataa atggctgcag taaaaagtcc ccgaaatcat gcgaaaatga ctggattctc
      781 tgccaataat agtgctgatc gtaacacaga aagcccgctg gggggcgggc tttctggaca
      841 ggaattggcg gatttgacgg tgttgtggag ccgggtctat ggccggacat taactgaaga
      901 tgaagtgctg gaaatttaca gaaatgtgaa acatctggca gaaacattaa tataaaggat
      961 tgaaacgatg aatacagtta tttgggcacg agtttcatcg cgagaacagc gggaaggata
     1021 ctcgattgat gcgcagctgc gggtttgtaa ggaaaaagcg cagcgggaag gatggaatgt
     1081 ggttcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntacg ctggaaagag
     1201 gaagatgttg agaaggccat ttttgatgag ctaaaacaaa tggttatccc cgacaggcac
     1261 ttccgagaat ctttccgtcg cacgctggaa gtggcattgt cagattcatc tgaatatcag
     1321 cgccaacaag atcgccaaat gaaaaagcgg ctgtcggaaa tacagagcat gaaggaccgg
     1381 cttttgaccg catatctggg gagcctgatt gacgaaatga ccctacaaaa taagacgacc
     1441 gagcttgcca cagaggagaa gtccctgaaa atgaccctac aaatgacccc atacgaaccg
     1501 caaagtgacc gaataaaacg gattttgggt atttttgagt tctcgcaaaa tatcgaaaaa
     1561 tattggcgcg gttcaaactc tgccgaaaaa cgcaaactgc tggaaatcgt gagtttgaac
     1621 cgacatttaa gtgacgtaag tctttgtgta gaaaagagaa agccgtttga ctttttggcc
     1681 aaacggcttg ttttggagaa gagtcggggc gactggactt gaaccagcga cctcttgacc
     1741 cccagtcaag cactctagcc aacctgagct acgccccgtt cgaagttgcc aatataacac
     1801 cttaatagaa aatagacaac gattttcttt ggattttccc aagaccaacg cctgctcttg
     1861 cggtaccgag gggctgaaaa agatttgaaa tacccagttt tgagatttat aatgcctgcc
     1921 aaaggaaaaa gaggccaaag acaatgatat acaaaaccta tgggaaaacc ggcttgaacg
     1981 tcagtgcagt tggcttcggc ggcatgcggt ttgataccgc acgctcaaag gaagaaaacg
     2041 ccgccctcct gcgctatgca gtggataagg gcattaactg gctggatact gcttcgggct
     2101 actgcgatga ccagagcgag gatattttcg ggcttgccat tgggcaaatg gcttctcgac
     2161 gcagtgcatt ttatgtctct accaaaggaa tgcccgaaaa ctgccccacg gccgccaaag
     2221 cacaggaagc agtcgaaaaa tccctgaagc gtctccgcac ggactatatt gatttttatt
     2281 acgtctggtg cattcgccgc tggaaagaat atgaactggc gacaaggccc ggcggacaat
     2341 atgaagggct tctcaaatgc cttcagcagg gcaaaatccg tcatttggtc gtttctacgc
     2401 atctccgcgg tccagatgtt cagcgccttt tgggaaaaat ggagttcgaa ggggttcttc
     2461 tgggcgtcaa tatcctcaat tttatgtacc gctggcaagg tgtccaagcc gccagtgagc
     2521 tcggcttggg agttgcagca atgaatcctc tggcaggggg gattatcccc cggcatcacg
     2581 agcagttagg ttttttagca ggtcccggtg aaactcctac cgaagcggcc ctgcggttct
     2641 gtatcagctg tcctcagata acagtaacac tggttggttt tactctgcgg gagcatattg
     2701 atacggcttg caggatagtc gacagcgcgg aactgttcag ccaagaggat atagagcgta
     2761 tccgccggcg gctgacctgc agtatggaca atctctgtac cggctgcggt tactgtatgg
     2821 aatactgccc gcgcaatatc cctatttcca actacatgca atattacaat tacaagctgc
     2881 ttgataaacg gtctgatgac aaaatgattg agctgctcaa agagcagtac gaatggggaa
     2941 tgctggccga ccgtccagct ggtgccgagg actgtgtgcg gtgcggcagg tgtgaattgg
     3001 cttgcaccca gcatctggat attatccgcc ggcttgaaga aatagctgtc tgggaaaaga
     3061 gctgttcgcc ttgaaatcgc agcatatttt cggcaaatcg ggcaaaccgg tgtaacagcc
     3121 aagaaaatga aacacaaaaa gacaagggat atcgagaatc ttgttctgca aaacagtcct
     3181 ccgcacaact tcgtcggaag tttgcttttg tatctcaagg aacaaacggg tgcagtctgc
     3241 cttttgccga tgattgatat tcaggaatca ccggtatatt tttgcatttg ataaaatcct
     3301 tgacagaaac ctcgataagc cggtacgata tgcccataat agactcgtaa ttaaggagta
     3361 tgctggtgca agagcgaaac gatacgtata ttacatatcg cctgttgaca ggtcttggaa
     3421 tcattctgtt tgcagttgcc ggctgcaggg acaatccgga gacaaaagcc gccaaagctg
     3481 tccgcaagca gacggcggaa gcggttcgag tatcggctac agaaaaggat tacccagcag
     3541 ctcagcagaa ggtaatggcg gctttggtgc agaatcgcac cggcggactg actcaggatg
     3601 cagcactgtt ggccagtgga acaatagctt tggcaaatgg gcggcagatg cagtctgact
     3661 tgggtctgaa aacgctgcct ttaaatcaat gtgcagacaa cattcgaaaa acccttcggc
     3721 gtttggagca gctgctcctt gagaaaaaac gtatcgaggg gcttttggcc ggcggggatt
     3781 tagaagttgc cgagctgaaa aaactgcttg tcggtgacgc tgaacatccc ggactgcagg
     3841 cgcagctgga acaggccaat gaacagttga gccagctgct ttctcaaaag cagaagcttc
     3901 aggaagaaaa gaataaagtc caggcggttc tggatgagca gcaggcgcag gccgatgccc
     3961 tgctgcgcaa ggctgaaatg gctcgcggcg atgaaaagct gtcgctccag cagcaggcct
     4021 atgccgtttt gctgcagcgg aaagattttt atgttcaggc acaagcagcc gaaaatgcaa
     4081 taaaaaatct ggatgccgat acggctctgg tgcaggtccg gattgacggc atcaaacaga
     4141 gcatcgagca agttggccaa aaggctgcag caatagaggc atccgcagca cgcgtctcgc
     4201 tcaaacagca gatggcagat attgaacggg agattgctgt ttatcagcag tctttgacgg
     4261 cattggccga taaattaaaa aacgactttt cagcctatca ggagacagcc gatcgaacgg
     4321 cggcgatttt tgaagaggcc tcagcggaat ttgaaaagat tcattcaaat gatgcacagt
     4381 tttctgctgc aataggactg gccgaaagtg ctcatcgtgc tgccctcgcc ggcgaagccc
     4441 ctgtaaaaat tcagctcgat ctggctcgcc ggctccagaa catgacagaa accgcagatc
     4501 ccaactttgc cggcgtactg cagagtcggc tgtcgctgcc gcttcaggtt cagccggaag
     4561 taaaacagaa gatattggca ctttatgacc gggcaattga aaactatgag aaagccctgg
     4621 cacaagcatc gcgttttgga tctgaagccg aatgcagtct gctgaaatcg ctggtgattg
     4681 cttcccatga caaaatgaaa ttcgccgatt tatgccaaga ctttgatttg gccaatgctg
     4741 cagaaacggc gatgatggca ctgattcaaa aagggactga aaaaggagtc tgctttacgc
     4801 agtccgaagt gcttaagatc atcgaaagtg agggcctcaa ttacctgccg tcaatgccgt
     4861 taaatatgga agttttcatc gagggaaagc ggcaggaatt gtctgcttgg aaacggctgc
     4921 ccatcagcca gcaggaagcg gccgtcaacg aagctcttcc tcaaattgac gccctgatta
     4981 ctcaatacgg ccagcagatg gccgatgctc tcgaaccgct taaacaggaa atgctggccg
     5041 ccaaagaacg cggtttcaaa gaacctgtcc ctgcggcagg gggtcccggc gagccgaatt
     5101 cactctttta atgagcagac tgcggaatga ccgattggtt tctatcccgg ttgaaaaccg
     5161 ggattttttt gcgcagaatg gaaaggcgat ttaaaactca taaccagcca acaattgagc
     5221 cgaaacaagc aaatgaaaac tgctgaaacc attggagcgg aacttgcggg tctaattaaa
     5281 gggactgtca gaattgatct gtgcactcgg acggccttca gtaccgatgc cagcatctac
     5341 cacattctgc cgcagtgtgt ggtgtcgccc cgggatgagc aggatatcat tgccgttgtc
     5401 tcctatgcag ccgccaatca gattccggtt gctccgcgcg gagccggcag cgggctggcc
     5461 ggagaatcac tgaccagcgg cattgtgatt gactgccgcc gctttatgaa cgctattgtt
     5521 gatactgctc cggacggctc gtgggtgcgg gttcagcccg gtgttgttct cgataaactg
     5581 aatgctcatc tggtttcttt cggccgcaaa attgggccgg atccatccag ttccaatagg
     5641 gcggttatgg ggggtgttgt tgccaacaat gccaccggtg ctcattctct gcaatacggc
     5701 tatattgctg attatgtgga ctctgttcgc gttgttctgg ccgatggaac tcctgtaacg
     5761 ctgtccagct gtatttgccc tgaatcggct ccagcgccag ttgcgaaact ggcctccgca
     5821 tgcgctgaag ttcttggccc cagccgtgag cttatagaca aagcccagcc ggccaccaag
     5881 cgcaaccggt gcggctatac gcttgttaat gttgttcaga acggaaggct gaacttggct
     5941 cgactgatgg ttggttcaga aggtacattg ggggtgttta cggaagtaac cctgcgcacg
     6001 gtacctgttc ccgctgctgt cggattgatt cagttcgagt ttgcttcaat ggaggcaatg
     6061 gctcgagctg ttccgattat tgtccgttcc ggtgcggctg cctgtgaact catggaccgc
     6121 accctgatgg atatggctcg gcaggccctg ccgcgatacc gggatgtgct gccggccggc
     6181 tgtgcggcag ctctgctggt agagcatgtc ggagatgcag ccggcgaggt gtcggccaag
     6241 attgagcata caatttctgc tgttggaact ttggctgctg acgccatgaa gattcttgag
     6301 cctgatcggc aggccttttt gtggaagtca cgcaaggatg ctgttccgct gctcaaccgg
     6361 caaaaaggac catcccatcc ggtggcattt attgaggatg tctccgtaga ttaccatcat
     6421 ctggagaaat acatcttagg gcttcaggaa atcggccggc ggtatgatat ccctatggca
     6481 ttctacggcc atgcggggga tggggagctg catattcgtc cctatctgga cctgagccgc
     6541 ccagaggaaa tcgaaagaat gcgtcgtatt gccgaggagg tatttgcact ggcttggtcg
     6601 ctaggcggct caatcagcgg ggaacacggc gacggcctgc tgcgggccgc ttttctcgaa
     6661 ggccagtatg gaaaggatta ttgtcagctt ctccgacaga tcaaacgcat ttttgatccc
     6721 gcgggcctga tgaatcccgg caaaatcctg tccgatgacc ccgatgttat gatcaaaaat
     6781 ctccgggcgc cggcccttaa agcagcggag gattttcaaa cccaattatt ctttaagcct
     6841 caggaatacc gttatgaagt cgaacaatgc aatggctgcg gtgtttgtct ggcccgcacg
     6901 gccggctcca gaatgtgtcc ggtttttcgg gggctggatg atgaactggc ttccagccgt
     6961 gccaaagcta atctgattgc tgcttggatg gcacgccagc cgctggaagc tgctgccctc
     7021 aaggatatgc tgagcttgtg tatcaattgc aaaatgtgtt ctattgattg tccggcgggg
     7081 gtagatgtat caaaactggt cattgaagcc cgtacccaat tggcccgcca aaccggtttt
     7141 tcagcagccg aaacagtcct ttccaataat cggcggctga gcatgcttgc ctctgccttt
     7201 tcgcctctga gcaatagggt gttgtcgctg ggtggggtcc gatggcttct ggaaaagctc
     7261 atcggacttg accgaaaccg gcgatttccg cattttgagc atggcagttt tatccgaaaa
     7321 gcccgtcggc atcttcagaa acagccgccg cttgctgagc cgatagacaa ggcagtttat
     7381 tttgtggaca gttttgccaa ctggaacgac cacgaacttg gctttgcagt attggatgtt
     7441 ctgaacaaat taggcatcca gacggctgtt cccccccagc gtccagcgcc gctgccggca
     7501 tatgtgtacg gcaacctcaa aaccgcccgc aaagatttgg aatataatgt ccggcagctt
     7561 ttgccctatg ttcagcaggg gtataaaatc gtttgttccg aacccagtgc tgcgctgtgc
     7621 ctgaaggaag agatgcggtt aattctcaat acagaagctg ctgcacagat tgcccggaat
     7681 acagtggagc tgatgtctta cattgaggat atatggtgtc gcaataaaag ggcagtcctg
     7741 gaaaacaaga atatctccgg ctgggcaggg aaaaagtttg cgtaccatgc cccctgtcat
     7801 ctcaaggcat tgaaaagcac ttcagccgct ctgaaactcc tcaacaagtt cggtgtaaat
     7861 gcagtcgatc tcaacgccgg atgctgcggg ctggctggta ccgccggaat gcagaaaaag
     7921 catcgcgatc tttcgatttc tgtcggcaga cctctggccg aagccatttc ggctgccggt
     7981 gctgatatta tcctgacgga atgcgccgcc tgcggaatgc agattgagca tctttcggga
     8041 aaaccggcga tgcatccgat taaaattctg tgccggatat ggcaggaaaa tgcttgctaa
     8101 tcagctcgtt ggcggttacc ttgtttaaaa aacatatata gatttttcgg attagggttg
     8161 ggctatgaag ttgacggatg tgctgcggta cgaatgtatc caagccggct ctgcggttga
     8221 tgacaaagca atggctttat gtgaaattgc atctctggcg aaaaaatgca ggctcttgaa
     8281 atctgtatcg gaagaaacca ttctcgaagc ccttcaggag cgggaaaccc tcggcacaac
     8341 cgccttcggc cgcggtattg caatccccca ttgccgtctg ccgggcctga gggattttgt
     8401 agtaggggta ctgaccagtc cgcagggaat cgaattcgaa tcggaagacg gccagaaggt
     8461 tcatctaatt gtctttatta ttgccccgca cgaacagagc gaccgtcata tccgtctgct
     8521 ttcgatggtt tcccgcatgc ttcaggatga ttctgtggtg gagaaattgg tcgaggctaa
     8581 atcagccgac gaggtaaaag ctgtcctgtt tgagtctgcc agagatgacc ttgccgagca
     8641 gcttcctgtt gtacgctgcc agattcatat ctttgttcag aatgaaaaaa tctttcgcag
     8701 cattctcgaa aagcttgccg ggctggatga tatcctgctg actgtaatgg atgccaaacc
     8761 gacccgttcc tatctgtcga aaacctcatt gtatgcggat ttttccaaaa acggcaaagc
     8821 agtatgcaaa gtggttgtgg cggttgttga gcacagccta accaacgaag tgatacggcg
     8881 tgttgaaacc atcaccggtc ccctgcttga atgcactgga gtgatggtaa cggttcagga
     8941 attaagctat tttgccggct cactggagat gtagcttggc gttttcatcc ggctgttatt
     9001 atccccaaag gcatttcgcc ggatccagac gaaaacaaaa gcaaaaaggt gcaacaggct
     9061 ttctgaaggt atttcagtct cgttcaggat gcagcaagag atttatggcc gacaagcgaa
     9121 ttcagatata tcataatctt tccgtgatgc tgaatgcggg agttcctatt gttcgtgctt
     9181 tgcggctggg acatagcacg ggcaaatacg ggcgtttgtt ttcgtgtata gagcaggatg
     9241 ttgccggcgg cacagcgctg gccgatgcat tcgaaaagca caacaaggat tttgcccctt
     9301 tggacatagc cctgattcga gtcggagacg aaacaggcca aaccgccgaa atgtttgaag
     9361 ccctttctca atggtacgcc tttcgccaga ggatgataaa cacagtccgc ttcggacttc
     9421 tgctgccggt cttctacatt cacgcggcgg cagttcttat cccctttgtc tcctttgcac
     9481 tcagcgggtg ggactggagt attttttttg aggggatgtt tgctattttg gctcttgctt
     9541 atgttccggc attgacaatt ctggctgtct tctatttaac accccgtcag ggtttattac
     9601 gccgcattct ggatcagata atcctgataa ttcctcttgt cggaaaagca gtcagagaat
     9661 tatcacttag ccgctattgc aatatatttg caattacata taaagcaggc ctgcccattc
     9721 tgcaatgtgc aaagatggca gcagattcca tcggcaatag cgtcctccgc agccggttaa
     9781 ccggtgcgta tgaaaatgta aaagcaggcc gggaaatgag cgacggcttc agtcggtatc
     9841 ttcctgctga gtttatcaat atctggcagg ttggagaaga aaccggccag ctggatgaca
     9901 gtgcttatcg gctggggaat atgcacgctg aaaatgcaca gcggcatttc acaatgcttg
     9961 ctaaaatagt tccatttgtt atctatctta tcgtaatttt tttgattgcc tttttcatta
    10021 ttatggctta ctcaaagctt tacggcacat taacagcata atgcgataca gattatgcag
    10081 aataaaaaat tctttacttt tggagtattt tttctttttt ttatcctggc tatacgatat
    10141 aatgctatcc tttggagagt atcctttggt ctctcaaggg tagtctcctt caattaaatc
    10201 gagagacaac agttagacgg tctctatgag accaaggagt acagtagtga gtacaggtac
    10261 agtaaagtgg ttcaacgcga gtaaaggttt tggtttcatc aaaccggatg acggcggcga
    10321 tgatttgttt gtgcatttct ccgaaatccg gatgcagggc tatgcaagcc ttgatgaaaa
    10381 ccagaaagtc agttttgaag tggggcaggg caaaaagggt ccgtgcgcga ccaatgtagt
    10441 ccccgcctaa ggttcacagc ttgacaaact cgaaaggccc tgtctttgga cagggccttt
    10501 tttatttaac tctcttccaa tcaaatcttt agcatataag ctgcgatagc cgataataat
    10561 cacaggatat atcgggagtg tttagtttgg aacgtttatt ttttcgaaaa tcgcagcgag
    10621 tggtatcgga acgggaattt gctaaagtac tgtcgcagaa gtgctttgtc tgctcgcaaa
    10681 tgctgcggct gtatgcagcc cccaacaacc tgaacatacc ccgtttcggc gtatcggtcg
    10741 gcagagcgat aggtaatgcc gttcttcgca accggctcaa acggcttgcg cgtgaggtct
    10801 ttcggcttaa tcagcatcaa attcccgcag gctatgacta tattttgatt tttacgcaga
    10861 aaatgccgaa acaaaaagca ggaacggcta agccaaaaaa acaggaattg tctcaaatgc
    10921 aggttgagga cttggagcat ctttttttgt ctatgctcca taatctcata aaaaagtcta
    10981 ataaagcaga aaataaataa ataatgaata ccatagccga aagccagacc agccataacg
    11041 agcagcctaa gttgatgcag ataggtccgg cggcccgaaa ggcgggtgtg tcgcggcagt
    11101 cgcttcagta ttatctgatg gttggattgc tcgagccgac tgagatttct cctaccggac
    11161 gtcgtctgtt tgaccaacag gcaattgaac ggattcggct gattcacaag ctcaacaaga
    11221 gcggctatcc gctgcgagca atacgggaac tctttgtcaa aagccagcaa gacaggaagg
    11281 gattagaaaa atgaccgatt atcaaaccag acagcggcag aaaaatatgg tcgtcggagt
    11341 ctttgttctt attgcattga ttgctttttt atggatgctg ttccgcttcc gtgaccttcc
    11401 actggctgtc agcaaaataa aatcatttgt tgttctggtc tattttcctg aagcgccggg
    11461 ggtccagaaa gatacgccgg tcaattactg cggctatcag ataggccggg tgatgaatgt
    11521 cgctccaccg caggtgtacg aagtgaacgg ccttaaattc caccgggtcg gggttacaat
    11581 ggcaatcgaa aaccgctata ccgatatacc aaaaaatgtt gatatttatg tgatgaagcg
    11641 aggacttgga agcagctata ttgaactggt tcaggatccg cagaaaaccg cattggcaga
    11701 aggattttta gaagataaaa tggtgctgga aaacggcaaa gtagggacaa ccagcgactt
    11761 tttcccgccc cagatacagg ccaagctgga aaatttggtc gattccattg cagcgctggc
    11821 ggataatgcc aatcagataa taggagatca ggataaccag accaatatta agaaaatgct
    11881 ggccaacata gaagccgcta ccgcacaagc cgataccacc cttaaatcga ttcagcagtt
    11941 ttccgatacc ggcaatgaaa aagtacggca aatatccgac aaaatcggac ttgctgccga
    12001 gcagctggaa ggggcgctgt ccgaattccg gcaggtaatg gcaaaaatcg aatccggtca
    12061 aggtactgca ggtaaattgc tgaatgacgg tcggctgtat gaaaacctca tcgaaagcag
    12121 ccgggagctt caaatggctc ttcagcagat aaaagaatgg gccgccgatg cccgcgaaaa
    12181 aggcatccgc atcaagtggt aagctcttga aatgacttgg attgattgct cagcctgtgg
    12241 tcttcaataa atttgttgct tgtacttttc cagccggcag cataagaggt gccggaagtt
    12301 tttttcaata tttggattag ccctaacgga aagctgcaat aggtacaggg ggggcaataa
    12361 aatctgtaaa tattgaccgg cgttggcaaa tccccgattt gtgccgcttt aggggccttg
    12421 cctgataccc tccattgcga atatccagac taacagcaac agcgttatgc aggagtagaa
    12481 tttgataagt acaaacccga actattttca ctattccata atgggactaa atatatctgt
    12541 aatggggtgt ttttgacaaa ttttcataaa aaaaggcgtg catttgacag ccaaatatgg
    12601 gagcataata gccatcaagt cttgggatgg agagatttca gtaaaaaatt tccatttttt
    12661 ctcttaattt cttgacaggc atagggttta tcaagtacct tatttgtcct gtcggctgcg
    12721 taggccgaga agcagtatag gcaagaagtc aatgtaattt taattgcagt ggaggtaagt
    12781 ttctgtgaat aaggaacttg cccgcgagct aatcagcgcg ggcgtacatt tcgggcatgc
    12841 ggtcagccgc tgggatccaa agatgaagcc gtacatcttc ggcaaacgcg ggatgatcca
    12901 tattatcaat gtaaaagaaa ccttaaaggg gctgatgatt gccaaaaagc tccttgccaa
    12961 tgtggtctcc aacggccgcg atgtggtatt tgtgggtact aaacggcagg cgaagaaagc
    13021 cgttcaaagt gcggccgaaa agtgcggaat gcactatgtg tctgaacggt ggctgggcgg
    13081 cacattgacc aatttccgaa ccattcgttc ccgtcttcag cgtctggagg aactggaagc
    13141 gatggaggcc tccggccaga ttgaatccca aagcaagaag cagggggcgc gtctgaaacg
    13201 cgaacttgcc aagataaaaa ccaaccttga aggcattcgc cggatgagcc ggatgccggg
    13261 ggctatggtg gtggtggatg ccaaaaagga gtatatcgcg ctgcgtgaag cacggaaatt
    13321 gggcattccg actattgcca taattgatac cgattctgat ccggataccg ttgatgtggc
    13381 aatcccgggc aatgacgatt ctatcaaggc aatcgagatt ctgctggacc agatggcttc
    13441 ggctgtggca gagggcaaga caatggtcaa gcaggaggat gtcacggcag cggcaggagc
    13501 acgtgccgcc cgtacgcgtt cccgccgccg ggttttagcg gctgcccagg aaacgcagga
    13561 gcagacagaa gaaaagagca gtactgaggt ctcccagccg gccgagagcc ctgctcagta
    13621 acagtattcg gtgattaaaa cgactggccc cggcagatgc ctcaacagat gcaggggccg
    13681 gctttttttc atcggttatg tacagaataa acagacgaaa tatcaggagt tagacaatgg
    13741 cggaaatttc agcggcaagt gttgttaaac tgcgcaggat gagcggacag ggtatgatgg
    13801 attgtaaaaa agcccttgaa gaggccaatg gggaccttga taaggcaatg gagctgcttc
    13861 gcaagaaagg gttggccaca ttggagaaac gagccggccg tgaaaccacg gaagggcgcg
    13921 ttctttgcag cacacgaaac gacggcaaaa cagcggtcat ggtgtcgctt tgctgtgaaa
    13981 cggattttgt cgccaaaagc gatgatttta ttcaagccgc ggaggctttg ctggaatgcg
    14041 gttttgcagc agcggctgaa cagggcgcag ctgcactgat ggaagttgcc gtgggaggca
    14101 ggaaatttgc tgatattctg acagataccg tcagcaaaac cggcgagaaa accgaagtag
    14161 gtgattttgc acggtacacc gccggtccta acggcatcat tggcacctat gtccacttta
    14221 atcataaagt gggggccatg gcggaaatag aagccgacag tccaacggtt gcccgggcac
    14281 tcaaaacgat tgcggatgaa atatgcatgc acattaccgc gatgaatccg atggctcttg
    14341 acaaagattc ttttgatccg gctgtgatcg agcgggaacg cgctattgct gccgagcagg
    14401 tcaaaggcaa acctgccaac atcattgaca aaattgtgga cggcaagatt gccaagtttc
    14461 tcaaggaaca ttgtctaatt gatcagtcct ttgtcaaaga cgacagcaaa acggtggcgc
    14521 aggtgttgac tgaagcagcg aaagccgccg gcggaagggc aaaaataaaa cggtttgtgc
    14581 ggattgaaat cggctgatgc tgtgatataa tgacaggcat cggaaacaag agtttccgat
    14641 gcctttttta ttgcatctat gggaacagga aaaaacgatg acggcggtaa aatataagcg
    14701 ggtgctgttg aaactgtctg gggaaagctt ctgcaagccc ggcggctttg gtgtagaggg
    14761 tgaagctctt gaatcgatag cgcagcgaat tgccagaatc tgccggctgg gtccgcaggt
    14821 tgccattgtg gtagggggag gcaatttcct gcgcggggca gcgttttcgg aaaagagcca
    14881 tattccgcga acgacggccg attatatggg aatgctggcg acagtgatta atgcctgtgc
    14941 actgcaggaa acgctggaaa aaatgggcca gccgacccgt gtgtgcagtg cgatagaaat
    15001 atcggctatt tgtgaaccgt ttatccgtcg ccaggccata gcgcatctcg aaaacgggcg
    15061 tgtggttatt ctggccggcg gaacgggcaa tccgttcttt tccaccgaca cctgcgccgc
    15121 cctgcgggcg tctgaactga atgccgatat tatgattaaa gcgacgaaag tagacggtgt
    15181 ttatacggat gatccgatga aaaatccgaa agcgcatcta gtggagtcgc tgtcctatca
    15241 gcaggttctg gagcaaaatc tccggataat ggacccggcg gcggtcagct tatgccggga
    15301 aaaccatatc cctgtggtcg tcttgaacat tttcaaggag ggcgctttaa cggctgcact
    15361 cgaaggcaaa aaagtcggaa ctctgatttc ggatgtggcg taataagagt accaacggct
    15421 tgagaaatag aaaggaatgg tatgccgacg cgaaaaataa ttgccatgca tgaaagcgca
    15481 atggagaaaa cagcagattt tctccgcaat gagctcaaat cggtgcgaac agggcgagcc
    15541 tcgccgggac ttgtagaaaa tttaagtgtc gattattacg gttctccgac accgctgaag
    15601 tcgctggcga cgattgcggt gccggaacca tcatcgattg tgattaagcc gtttgatccg
    15661 gcctgtctaa aagatattga aaaagctatt aaaaccagcg atttgagtct ggctcctgtt
    15721 cttgacggta aaatgattcg cctgaatatt ccgccgctga gcgaggaacg ccgcaagcag
    15781 attgtccagc aagtcaagca gatgggcgaa aaaaccaaag tcagcatccg caatatccgg
    15841 cgggatgcca acaagcagct ggatgatgag cagaaaaaca agacaattac ggaagacgac
    15901 tgcaagaagg ccaaagagac aatagataca ttgaccaaaa agttcatcga tgaggtggat
    15961 tcgattatca agcataagtc tgaagaaatt atgaccaatt aatcggaaag gggccagcaa
    16021 tgagcggcca gacaagtagt gcagaaaagc ccaaaccatc aaaccgcagg gtatggttta
    16081 tcgtgtgggg gctggtttcc atcgccggct tggcagtgct gatattgttc gggataccgc
    16141 tgtttttgtc atccgccggc gggacgcaat ttctgttaag caaaatcaat caatcggtgg
    16201 atggaaaggt ccagatggac gatttctcta tcggctggtt caaaggcatc catctgacca
    16261 atctgtctta tgccgacaat gcaggaatgg cttcggtcaa agtccgccgg ctggaaaccc
    16321 agccgcaata ctgggccctg ctgggcggca aaatcaatct gggcaaaacg ctgattgaag
    16381 gccccgatgt ttatgtgaag attcctgcgg ttcaggaacc ggctggaaaa acccaaaagc
    16441 gtgaaagcgg gcagcaatcc gcaacgccgt ctgcttttcc gattcatcaa atcaatctgg
    16501 agcttgtgga cggcagggca acagtggaat taggcgggga tgtgccgcag gcggtttctt
    16561 ttacgaatat tgcttcggtt gtgaatctgg cagccgccgg acagcgatcc atgctggatg
    16621 tatccatgaa tgttgttggg cagggagagc cggcagccct gtcggccaaa gggcaggcaa
    16681 caaccgataa aaccggctgg aaactcaaag aaggcgcctt tgatgtgaag attgataagc
    16741 tgcagcttgc ctcgcttaag ccgttgtttg cactggcagg caagacagtt gatatcgcag
    16801 gtgtcttgaa tgccgaagga acgattgcaa ttcagaataa tgcagtcggg caagccttcc
    16861 ttacggcaac aattgataat ttttcacagg ggaccggcca gcagcagatt aaaaccgcca
    16921 agccgattac tgtgacggtc tcggctgccc agcaggattc gggaatcaaa attgacaaat
    16981 tcgctgttgc ttcggagttt ttcaatgtca attgttccgg caccgcagaa gcaatcgatt
    17041 atgctgttga cgccgattta gcacagaccc agaatctgat tgcccagttt gcggatatgg
    17101 gcgggctttc gatgagggga atgctggatc tgcgcggcaa agtcctgatg aaagagcagg
    17161 agattaaggc cgccggcaag gggaccgtcc gagaactgat tgtccggaag ggagatgtcg
    17221 aaacccccaa aacgaatgtt gatgcgctgg aatttgactg caccgtcgat caggccaaaa
    17281 atcagctccg ggtggccatg gccaatatga ccgccccgcc tgtgggtgca gtgaaaatat
    17341 cgaatgtggt ggttccaatg ggggaggccg gggaaa
//
LOCUS       DAOTBO010000059        17204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2756_length_17204_cov_5.123214, whole genome shotgun sequence.
ACCESSION   DAOTBO010000059 DAOTBO010000000
VERSION     DAOTBO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_17204_cov_5.123214"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLE88_07960"
     CDS             <1..955
                     /locus_tag="PLE88_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOM76435.1"
                     /translation="FLEDTGYMARAAFIMDRIMHKIGLHGKSFIPMLIGFGCSVPAIM
                     ATRILENRRNRLATMLVIPLMSCGARIAIYAIFAAALFPDYLRGPMMWLIYVIGIVLA
                     IGCIQLLRRTLLKGETIPFVMELPPYRLPTLKGLAIHMWDRGWMYLKKAGTIILALSI
                     ILWAAISYPQPPAASLEGLSSSSQQQAVLQYSLAGRIGKALEPVVAPLGFDWKIATAL
                     IGASVAKEVFVSQLSIIYALGDTAENAEALEEHLRSDYTPLQGFCVMLFCLITAPCAA
                     TVAVTCRESGAWKWAALQYFGLTALAYGITFIVYQTGRLIF"
     gene            complement(959..2941)
                     /locus_tag="PLE88_07965"
     CDS             complement(959..2941)
                     /locus_tag="PLE88_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOM76436.1"
                     /translation="MKRPSSYLQRNRISAYLFVVGLLVGVIGAEPSDQTDMPAVKFDL
                     NDPVQRQLFAETLAQQRQPGIEEARQFLLRTGRPKRWTDGNTVYELMAIENGQPIIFM
                     TLCVNSAVSIAADIVRDNPYYQIQGDGVLAGVWDEGVGRLTHQELAGRIIAKESTPAS
                     NHSTLVSGILAASGINPNAAGMAPLAVLYGYDWYDDTEEMGLAAMSSAGQADKIQISN
                     HSYGNVCGWHKENGTWSWYGNPAYQESDLFGQYNYLAEEFDHICWNAPYYLPFRAAGN
                     DRYDTSRPAEGQTYLINSTTSAVFTADSGPYADTWDNGGFDTILSGACAKNVMTVGSV
                     NDAVSGGSRDLSKAAVSTFSAWGPTDDGRVKPDIVANGYTVFSCSSAGDTAYDTASGT
                     SLAAPAAAGAACQLLDLYSRLFSGQSMTAAALKALMIHTGDDLGNPGPDYKFGWGLLN
                     NAKAAEKLLLHKRNPSAANIIEDKVYQTGNTNDTFVFEWDGISPIKATLCWTDPPASK
                     TQVGTLDDSTSKLVNNLGLLIMAPDDLTQYYPFVLDPANPGAAATRGINNRDNVKQVL
                     IEAPLQAGRYTVQISADNFRRGYTSQAYCLIISGQRQPALADIDGDGQIGLADVQTLA
                     QGWMTDNPAGDIFPALGDGQVDLLDFGLLANQWLQP"
     gene            complement(3055..4473)
                     /locus_tag="PLE88_07970"
     CDS             complement(3055..4473)
                     /locus_tag="PLE88_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOM76437.1"
                     /translation="MVRTRFCVAVFCAAGLLLSAPAPGLTEACGPAGTNAYALHRLGI
                     TGRGVSIGLLSAGTVRQSHIAFERKEGSAVSLYDFTGGGFAPSWHDTHIAGIILSGGS
                     PSHPDQIGAAPGAVLHSARISNKQITSAYITDALDELIFNKNCRIILTGLQITHDSVQ
                     PDGSSIWTKIYDYYADTYDVLFINAAGNSCPQITIFGDSYNGLTIAGLVKDQTGRFGK
                     IGSISNRGPTLDGRKKPELAAPTQDLLVPTSTGDDHWTGLDPNGLGLTSYAIPHAAAA
                     AALLLEAAGKTPQADDDKTEVLKAVLVNAADAAAFQLRSGQLSPTDSITQWKPDGGYG
                     RLDALRAYHILTAGPIAKDLPNSRQQGWAYALIEAGQQHQYGLEAKKGRRLVITLTWH
                     RKLQRISNRFYEEPDRFYLDLKIQSPSGKMLVFETPGRNNLIKTDCVLPETGRYAVIL
                     KNPTAASQRDYGLAFELLEPAE"
     gene            complement(4479..4853)
                     /locus_tag="PLE88_07975"
     CDS             complement(4479..4853)
                     /locus_tag="PLE88_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76438.1"
                     /translation="MRHIVWLAGAVTAGMAIAVILKPLWMRQMLVFLQKGKLAYPAAA
                     FKSAVGILFLIFATGCRIPWIIILVGILMTVGPILFCFLPFAKIQSYLNWWAARPAWM
                     YRLWAAAALLLGFLIIWAGWPY"
     gene            complement(4889..5437)
                     /locus_tag="PLE88_07980"
     CDS             complement(4889..5437)
                     /locus_tag="PLE88_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOM76439.1"
                     /translation="MKVNENPPMTWPEAASIAEFKGTWWVAHTKPRNEKALAWQLLSK
                     EVAYFLPMHWKVSRRHRRTFRSLLPLFAGYLFFCGSEDSRLEVLKTNRTAGIIPVENQ
                     SQLIKELLPIETLLKSGKPVLPHDYIQVGQRCRVITGPLMGTEGIVISTPRQTRLVLS
                     VQILGQAASVEIGWDMIEKIDG"
     gene            complement(5520..6479)
                     /locus_tag="PLE88_07985"
     CDS             complement(5520..6479)
                     /locus_tag="PLE88_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOM76440.1"
                     /translation="MSQMKLYPLKFKPIFKQRIWGGRLLRDFFGKDLPSGVHIGESWE
                     LADLPDDKSQIVNGPFNGQTIDQVLAAYPKEITGKPDYHPPFPLLIKILDAQDVLSVQ
                     VHPDIETCRRTGKGEPKTECWYIIDAQPGAVIYKGLKAGVTRSRFAQAIADGTCADYL
                     VKVPVHSGECHFLPSGTCHAIGAGLLIAEIQQPSDTTYRVFDWNRIDPTTGKPRPLHI
                     DDALEAIHFDSSGDTLTVRSVGRLADADEFKVDKGHQAAGCEVLLREQMKVLMILTGS
                     GQITAKGLEPVPFTKGDTLLIPAAFEGVMQFKEDCQYLVVLTG"
     gene            complement(6536..7471)
                     /locus_tag="PLE88_07990"
     CDS             complement(6536..7471)
                     /locus_tag="PLE88_07990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOM76441.1"
                     /translation="MQHNTKRLLAAVCILGCISLVGCGGSQRKTSAAVVPAYCLSIEQ
                     LAAKLGLSVQKAGMPYYELTDANNRVLLFLYDGGRIYVNGKAIGPVGTVTQVGGSYYV
                     SELLVPKIRGNLSAYTPSPAKPGSMDTQIPRSLTGLVVVDAGHGGHDPGASSYLGYYE
                     KGINLSIARKVASYLQQRGIKTIMTRDSDTFVELNERADIANRAGADMFVSIHADSNG
                     DRTHRGYTIYVARSASWASKKAGQNIENTMASTGLPSKGLRNADYRVLVRTDCPAVLV
                     ECGFLSNPSDAALLSDPQFQDRIARAIADGIVKSL"
     gene            complement(7600..8022)
                     /locus_tag="PLE88_07995"
     CDS             complement(7600..8022)
                     /locus_tag="PLE88_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOM76442.1"
                     /translation="MKQKAIDLLRSSNLRRTEPRIAILVAMLKADAPLTQEQIAEHIG
                     GQPPNKTTIYRTLMNLVQKNLVHRVFVEERTWHFELAHHCSLHQCHPHFTCRRCLQTQ
                     CLTDVAVPLLQLPEGYTIHRQQIRIEGICSACRNKPKK"
     gene            complement(8019..8786)
                     /locus_tag="PLE88_08000"
     CDS             complement(8019..8786)
                     /locus_tag="PLE88_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76443.1"
                     /translation="MRILGKLILIGKELRNHSPFTMLGAATGIIFMLAGQRWFKEQAE
                     AMFSVFHPLHVVLSAMVTAALFEIHRKAKNFLVILLIGVVGSIGVATLSDCILPFFGE
                     SILGVAVPTHAAVHEHSTAQNALPEQSAQQSEHIHTDQHHPRWHIGFIEEWYLVFPAA
                     LLGVLLAYFKPATHLPHASHVLISTWASSAHMLMNTQADLSLTVLLGMFIVLFIAVWL
                     PCCFSDIIFPLLFVRSDGAHIGHSCILCGKKEQNAVV"
     gene            complement(8940..9326)
                     /locus_tag="PLE88_08005"
     CDS             complement(8940..9326)
                     /locus_tag="PLE88_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOM76444.1"
                     /translation="MTQRKMIQTDKAPKAIGPYSQALLAGSTLYISGQLGLDPATGKL
                     VEGGTAQQARQALDNIRAILAAADMDMGNVVQVQVFLTEITDFAAVNEVYKSCFKEPY
                     PARAAFQAAALPAGAKVEILAVAVKN"
     gene            9653..10624
                     /locus_tag="PLE88_08010"
     CDS             9653..10624
                     /locus_tag="PLE88_08010"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOM76445.1"
                     /translation="MRIVETQQQMSSIPKGAVLTIGNFDGVHRGHQAILSTARRLARE
                     KGLAFAVMTFDPHPAVVLHPEQVLGILTPLPIKTLLFEAMSVDILIVIKDSMGLMNLS
                     PEDFVDQFLMKNLAPSAVVEGPDFNFGYGRSGSIHTLRELSASRGFEVVEVPFAEFFT
                     ENDRRGVKCSSTTIRHLLEAGDVWQASQILGRPYRLVGKTIAGRGIGRQLGFPTANID
                     PIKQVIPAEGVYAGYAVVGDSLEEVAFGGLRRPAAVSIGRAKTFVSDHPLLLEAHFLE
                     DKVEDLKGKWLALDFMRFIRHQQRFETHQLLQRQIAKDCQKALNFLL"
     gene            complement(10649..11401)
                     /locus_tag="PLE88_08015"
     CDS             complement(10649..11401)
                     /locus_tag="PLE88_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM76446.1"
                     /translation="MEQINPNIQKERNEFESAMSALPESLQPLLLSGKSVADLAHLAK
                     NIIQVVSGCAEIIELAMARNDYVRIQQSWGVFGPNLARLKKFVLDLIKYTRHYPIQKT
                     VCNLNEIIEKALHSCQPLLNKHAVKIQLHLDKSISPVQMDADRMEETLVNLLIHALDN
                     LPAHSGSISIQTLLLTNPPQIELRISDDGPALSDEQIRSLAEPAERSQNMCGTGFEIV
                     LSKLAVEQHDGYMEIASTPPKGNQIHIYLPLS"
     gene            11755..12609
                     /gene="panB"
                     /locus_tag="PLE88_08020"
     CDS             11755..12609
                     /gene="panB"
                     /locus_tag="PLE88_08020"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOM76447.1"
                     /translation="MDKKVTISDLMEAKQAGRKITAVSCYDYTTARLVAQAGIEMILV
                     GDSAAQVMLGYETTLPATMDFMVTITAAVRRAAPNVCLAADMPFLSYQTGLADAVRNA
                     GRFFQEAGVQIVKVEAASAHLDVVKAISDSGMAVMAHIGIRPQQIAKLGRLKAEGTTA
                     ELAMELVDLSRQMVQAGAGMLLLEGTAREVAKIVAQINPVPVISCGSGPDCDGQILII
                     SDILGLSGQAKPKFAKNFAHLDEPIVQAVAAYAQQVRQMQYPSDEHCYHIKSGQLERL
                     RQMLDEST"
     gene            12623..13762
                     /locus_tag="PLE88_08025"
     CDS             12623..13762
                     /locus_tag="PLE88_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM76448.1"
                     /translation="MPDPTAETASEITIKPVYLADKGVLQSYSCYIRRILVGLADTAR
                     GAVVVCPDHGQAEGVLCPSVERIEHPALRLPIFWHQNRRLLLDRLLRQRPTLLHAFYP
                     GQIHLAQWLADELDVPYVITFHQAPSRWMRFEKPIRHAACIIAPSGPIAERLKKQWPG
                     LTERIVCVPVGSFAEDQCSCFSRPGTIPSLVAVQPLDSFEVVKPLLNAIRHLALDGLE
                     LIAAIMGQGRAERDIRRHIRSLGLTSVVALVPPMHPVRDVFSGADIYLHLQDRGCFDA
                     QLLEALAVGLAVAGCREESSGLLIEGQTALFWDAHDELSIYSCLKTMLTQREKTRQLA
                     QNAQAYLSEHHSVSRMVERLAEIYAQAQLNRGFPEPTGQAAPQSA"
     gene            13795..14808
                     /locus_tag="PLE88_08030"
     CDS             13795..14808
                     /locus_tag="PLE88_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOM76449.1"
                     /translation="MKQMSGLFKTLIGWYTKLFAFWILLFAAVGYFFPKPFVFLNSCK
                     MFGPWADRWPAVFQSLRSPNLWFFALTMFGIGAVLTINDFKNIAKRPVIVLIGSIAQF
                     TIMPVGAYAIAKAFHLPPLLGAGLIIAGCAPGAMSSNVMSYLAGADVAYSVSLTTVST
                     LLCPLLTPALTKLLAGTELPVSFWKMFWEIIFMVIVPLMAGFAVRYFFGRFVERIKDV
                     FPAISVTFIIFVCTLVIASNREKMLDATGLIVLAVLLLNLGGMAGGWVVGALFGMRFD
                     QRRTLAIEVGMQNAGLGTVLAIHNLGPEAAIPTAFFVFTCIITASVMTEIWKGRKRAK
                     NAF"
     gene            14824..15633
                     /locus_tag="PLE88_08035"
     CDS             14824..15633
                     /locus_tag="PLE88_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOM76450.1"
                     /translation="MAYIHGYSQREMQRLYEQAEILEEILHTGTLFPAGSRVLEAGCG
                     VGAQTRLLVKRNPDAVFTCIDISEKSLAAARRLKEQAGFEHVSFQKEDIHNLSFADET
                     FDHVFVCFVLEHLDAPAAALKELKRVLKKGGTITIIEGDHGSCFWHPQTPESIAAWYG
                     LIKAQQNIGHDPTIGRRLTPLLTEAGFRLQSCRPAWLYADRLNPALRDGMVNHIIVPM
                     VQSAETQILADNLVPQDMYQKGIADLLRVDEVEEGTFFYTWFKAVAGKNGM"
     gene            15683..16834
                     /gene="pdxB"
                     /locus_tag="PLE88_08040"
     CDS             15683..16834
                     /gene="pdxB"
                     /locus_tag="PLE88_08040"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF001309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HOM76451.1"
                     /translation="MKIVADQNIPFVKECFSSLGEVVLVSGRQITPALIRDADALLVR
                     SITKVDKDLLAGSRVQFVATATIGFEHVDRDYLTANGIGFASAPGSNANSVAEYITAA
                     LLALGRKYKFRLEGKSIGIVGVGNVGSRVEKKCRALGMKAVLNDPPLARQTGDAKYRP
                     LEELFDCDFITLHTPLTKEGPDKTYHLADERFFASLKNGAVFLNTSRGKVHDEAALKS
                     AMKAGRLRAVVLDVWETEPNVDSWLLRYVDISTPHIAGYSFDGKVVGMLMVYEALCRH
                     FGLKTVHTAAEFLPDPAVPEIRITEQQDSEQIIHHTVQQVYAIHRDDFNMREILLQPK
                     EQRGAFFDNLRKNYPIRREFQNTAVLLADTQSPLARKLEGIGFRTGVLR"
     gene            16831..>17204
                     /locus_tag="PLE88_08045"
     CDS             16831..>17204
                     /locus_tag="PLE88_08045"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOM76452.1"
                     /translation="MKPLPDIVIWPGGRLDFSGGCLVMGILNVTPDSFSDGGRYLDAD
                     AAAAHGIEMAKQGAAIIDVGPESTRPGSQPVSAGEQIRRAVPVIEKLAKQIDIPISID
                     TRNPEVAAAAVEAGASMVNDITA"
BASE COUNT         3853 a         4206 c         5004 g         4141 t
ORIGIN      
        1 ctttctggaa gataccggat atatggcgcg ggcggctttt attatggacc ggattatgca
       61 caaaatcggt ctgcacggca aaagttttat cccgatgctc atcggcttcg gctgctcggt
      121 gccggcgatt atggccaccc gcattctgga aaaccggcgc aaccggctgg cgacgatgct
      181 ggtaataccg ctgatgagct gcggggcacg gatcgccatt tatgcgattt ttgcggctgc
      241 cctttttccc gattatctgc gggggccgat gatgtggttg atttatgtca tcgggatagt
      301 cttggcgatt ggctgcatcc agctgctgcg gcggacgctg ctgaagggcg aaacaatacc
      361 gtttgtgatg gagctgccgc cgtatcggct gccgacgctc aaagggctgg ccatccatat
      421 gtgggaccgc ggctggatgt atctgaaaaa agccggaaca atcattctgg cgctgtcgat
      481 tattctgtgg gcggctatca gctatccgca gccgccggcg gcgtctctgg agggcttaag
      541 cagcagcagt cagcagcagg cagtgctgca gtattcgctg gccggacgca tcggcaaggc
      601 tttggagccg gttgttgcgc cgctgggatt tgactggaag atagccacgg cgctgattgg
      661 ggcgtctgtt gccaaagagg tgtttgtctc acagctgtcg attatctatg cgctgggcga
      721 tacggccgaa aatgccgaag cgctggaaga gcacttacgc agcgattata ctccgctgca
      781 gggattctgc gtgatgctgt tctgtctgat tacggccccc tgcgctgcaa ctgtggctgt
      841 aacctgccgg gagtcagggg cgtggaagtg ggcggcactg cagtatttcg gcctgacggc
      901 gctggcctac ggcattacct tcatcgtgta tcagaccgga cggctgattt tttaaccgtt
      961 acggctgcag ccattgattg gccagcaggc cgaaatccag caggtccacc tgcccgtcgc
     1021 cgagggccgg aaagatatcg cctgcgggat tgtccgtcat ccatccctgt gccagcgtct
     1081 gcacatcggc caacccgatt tggccgtcgc cgtcgatgtc ggccagagcc ggctgccgct
     1141 ggccgctgat aatcaggcaa taggcctgcg atgtatagcc gcgccgaaaa ttgtcagcgg
     1201 aaatctgcac ggtgtagcgg cccgcctgta gcggcgcctc gatgagcacc tgcttgacat
     1261 tgtcgcggtt gttgatgccg cgtgtagcgg cagcgccggg attggccgga tccagaacaa
     1321 acgggtaata ctgtgtcagg tcgtcgggag ccatgataag aagtcccaga ttgttaacca
     1381 gcttcgaggt gctgtcgtcc agcgttccaa cttgtgtctt gcttgcaggc gggtctgtcc
     1441 agcagagggt ggccttgatg ggactgatgc cgtcccattc aaagacaaat gtatcgttgg
     1501 tatttccggt ttggtaaacc ttgtcttcga taatgttggc ggcggatgga ttgcgcttgt
     1561 gcagaagcag tttttcagcg gctttggcat tgttcagcag tccccagccg aatttgtagt
     1621 ccgggccggg attgccgaga tcgtcgccgg tatgaatcat cagggctttg agtgctgcgg
     1681 ctgtcataga ttgacccgaa aacaggcggc tgtaaaggtc cagaagctga caggcagcgc
     1741 cggcagcggc aggggcggcc agacttgtgc cggaagcggt atcgtaggcg gtatcgccgg
     1801 cggaactgca ggaaaagacg gtatagccgt tggcaacaat atccggcttg acacggccgt
     1861 cgtcggtcgg tccccatgcg ctgaatgtgc tgacggcggc tttggacaag tcgcgacttc
     1921 cgccggagac ggcgtcattg acagagccga cggtcattac attcttggcg caggccccgg
     1981 acaggatggt atcaaatccg ccgttgtccc aagtatcggc ataaggtccg ctgtcggctg
     2041 taaaaacagc cgatgtggtg ctgttgatca gataggtttg gccttccgca gggcggctgg
     2101 tgtcgtagcg gtcattgccg gcggctcgaa acggcagata gtagggagca ttccagcaga
     2161 tgtggtcaaa ttcctctgcc agatagttat actggccgaa caaatcgctt tcctgataag
     2221 ccggatttcc ataccaagac catgttccat tctctttgtg ccagccgcag acatttccat
     2281 aggaatggtt ggaaatctgg attttatctg cttggccggc tgaactcatt gccgccaatc
     2341 ccatttcttc tgtgtcgtcg taccagtcat atccatagag gacggccagc ggagccattc
     2401 ctgcggcatt ggggttgatt ccagaagctg ccaagatgcc cgaaaccaat gtagaatgat
     2461 tggacgcggg ggtgctttct ttggctataa ttcttcctgc aagctcctga tgtgtcagcc
     2521 ggccgacacc ttcgtcccat acgccggcca gaacaccgtc tccctgtatt tggtaatacg
     2581 gattatcgcg gacaatatca gcggcaatgc tgacagcgga attcacacag agggtcatga
     2641 agataatcgg ctggccgttc tcgatagcca ttaattcata aacggtattg ccgtcggtcc
     2701 accgtttcgg ccggccggta cggagcaaaa actgccgtgc ctcttcaatg cccggctgcc
     2761 gctgctgggc aagcgtttcg gcaaaaagct gccgctggac tgggtcattg aggtcaaatt
     2821 tcacagcagg catgtctgtt tggtcagatg gctctgcgcc gatgaccccc accagaaggc
     2881 caactacaaa taaatatgcg gaaatccgat tcctttgcaa ataagatgag ggtcttttca
     2941 tcaccgcatt ccacttaatc agctattacc ctacatcaga ataacaaaat cgaaggagaa
     3001 aaacaagaca attataatct atagaaaggg attacagcaa agtacccgcc tgtcttattc
     3061 agcaggttcc agcagctcaa aggccagccc atagtcccgc tggcttgcgg cagtcggatt
     3121 tttcaggatg actgcatagc ggcctgtttc cggcaaaaca caatcggttt ttataaggtt
     3181 atttcggccg ggtgtctcga aaaccagcat ttttccggag ggggattgga tttttaaatc
     3241 cagataaaac cggtccggct cttcataaaa ccggtttgaa atccgctgga gttttcgatg
     3301 ccaagtgagg gtgatgacca gccggcggcc tttttttgct tccagtccat attggtgctg
     3361 ttggccggcc tcaatgaggg cataggccca gccctgttgt cgcgagttgg gcagatcttt
     3421 ggcgatggga ccggccgtca ggatgtgata ggcccgcagg gcatccagcc ggccgtagcc
     3481 gccgtcgggc ttccattggg tgatagaatc tgtcggggaa agctggccgc tgcggagttg
     3541 gaacgcagcg gcatcggccg cattgacaag cacggctttg aggacttcgg ttttgtcatc
     3601 atcggcttgc ggggttttgc cggccgcttc cagcaggagg gcggcggcag cggctgcatg
     3661 cggtatggca tagctggtca atcccagacc gttgggatcc agccctgtcc agtggtcgtc
     3721 gccggtgctg gtggggacaa gcaaatcttg ggttggggcg gccagctccg gttttttgcg
     3781 tccatcgagc gtagggccac gattgctgat tgatccgatt ttgccaaatc tgcctgtttg
     3841 atcttttacc agtccggcta tcgttaggcc gttgtacgaa tcgccgaaaa tcgtaatctg
     3901 ggggcaggaa ttgccggctg cattgataaa gaggacgtca taggtgtcgg cgtagtaatc
     3961 gtatattttt gtccaaatag agctgccgtc gggctggacg gagtcatggg taatctgcag
     4021 accggtcaga atgatgcggc agtttttgtt gaaaatcagt tcgtccagtg catcggtgat
     4081 ataggcggaa gttatttgct tgttggaaat gcgggcgctg tggaggacag ccccgggtgc
     4141 agcgccgatt tgatcggggt gactgggcga gccgcccgac aagataatgc cggcaatgtg
     4201 ggtgtcatgc cacgaagggg caaaaccgcc gccggtaaag tcgtaaagcg atacggcgct
     4261 gccctccttg cgttcaaagg cgatatggct ttggcggact gtgccggccg aaagcagacc
     4321 gatagaaacg cctctgccgg tgatgccgag ccggtggagg gcataggcat tcgtgccggc
     4381 aggcccgcag gcctctgtta agcccggcgc cggagccgaa agaagcaatc ccgccgcgca
     4441 gaagacagcc acgcagaatc ttgttctgac catatttctc aataaggcca tccggcccag
     4501 ataatcagaa aacccagcag cagcgccgcg gccgcccaca gccgatacat ccatgccggc
     4561 cgggctgccc accagttcag ataactctgg atttttgcga acggcagaaa gcagaagaga
     4621 atcggcccca ctgtcatcag gatacccacc agaatgataa tccacgggat tctgcagccg
     4681 gtcgcaaaga tcagaaagag aatccccaca gccgatttga aagcagccgc tggatacgcc
     4741 agtttgcctt tttgtaaaaa gaccagcatc tgccgcatcc agaggggttt caggatgacc
     4801 gctatggcca taccagccgt aacggctcct gccagccata caatatgcct catcgccaat
     4861 gctcctttcc ttgtgaaaac gattgggctt aaccgtcgat tttttcaatc atatcccagc
     4921 cgatttccac gctggccgcc tgcccaagaa tctgcacgct cagtaccaga cgggtttggc
     4981 ggggagtgct gatgacaatc ccttcggtac ccatcagcgg acctgtaata acccgacacc
     5041 gctggccaac ctgaatgtaa tcgtgcggca ggacgggctt gcctgatttc agcagggttt
     5101 caatcggaag cagttctttg attaattgac tctgattttc taccggaatg atgccggctg
     5161 tgcggttggt ttttaagact tccagacggc tgtcttcgct gccgcagaaa aaaaggtagc
     5221 cggcaaataa cggcagcagt gagcgaaatg tccgccgatg ccggcggctt accttccaat
     5281 gcatcggcag aaaatacgcc acctcttttg acagcagctg ccaggcgagg gctttttcat
     5341 tgcggggttt ggtatgcgct acccaccatg ttcctttaaa ttcggcaatg gaagccgctt
     5401 cgggccatgt catcggagga ttttcgttta ctttcaacac agctgctgac tccccggtct
     5461 gttctgagtt ctattgtatc gaaaaagggc gtgcatatca acaggttcaa atcagcacat
     5521 catccggtaa gcacaacaag atattggcag tcttctttga attgcataac gccttcaaac
     5581 gctgcgggaa tcagcaaggt atcgcctttt gtgaacggaa cgggctccag tcctttggcc
     5641 gtaatctgtc cagagccggt cagtatcatc aggaccttca tttgttccct cagcagcact
     5701 tcacagccgg cggcttgatg gcctttatcg accttgaatt cgtccgcatc ggccagacgt
     5761 ccgaccgagc ggacggtcag cgtatcgccg gacgaatcga aatgaatggc ctccagagca
     5821 tcatcgatat gcagggggcg gggtttgccg gttgtcgggt caatgcggtt ccagtcaaag
     5881 acccgatagg tcgtatcgga gggctgctga atttcggcaa tcagcagtcc ggctccgata
     5941 gcatggcagg tgcctgacgg aaggaaatga cattcgccgc tgtgtaccgg cactttcacc
     6001 agataatcag cacaggttcc gtcggcgatg gcctgagcaa accggctgcg tgtaacgccg
     6061 gctttgaggc ctttgtaaat gacagccccc ggctgggcat cgatgatata ccagcactcg
     6121 gttttgggct ctcccttgcc ggttcgccgg caggtttcga tatcgggatg gacctgaacg
     6181 cttaaaacat cttgggcgtc aaggatttta atcagcaggg gaaacggggg atgataatcg
     6241 ggttttccgg ttatttcctt tggatatgcc gcaagcacct gatcaatcgt ttgcccgttg
     6301 aaaggcccat tgacaatttg ggatttatcg tcgggcaggt cagccagctc ccagctttct
     6361 ccaatgtgca cgccggaagg caggtctttg ccgaaaaaat cccgaaggag ccggccgccc
     6421 caaatacgct gcttgaaaat cggcttaaat tttaaggggt ataacttcat ctgcgacatt
     6481 gtagcggcaa tatccgatat gtccagtatc ttctgggcaa acaggcagtt tggttttaga
     6541 gacttttgac aatgccgtca gcaatcgcgc gagcaatcct gtcctgaaac tggggatcgc
     6601 ttaaaagggc ggcgtcggaa ggattcgaaa ggaatccgca ttcgaccagt acggccgggc
     6661 agtccgttcg aaccagcacg cggtaatccg catttcgcag gcccttgctg ggaagccctg
     6721 tggaagccat cgtgttttca atattctgac ctgccttttt ggaagcccag ctggctgaac
     6781 gggccacata aatggtgtat ccccgatggg tgcggtcacc attggagtcg gcatggatgg
     6841 agacaaacat atcggctccg gcacggttgg cgatgtcggc tcgctcgttt aactccacaa
     6901 aggtatcgct gtcgcgggtc ataatcgttt taattccccg ctgctggaga taagatgcaa
     6961 cttttcgggc gatactcagg ttgatgcctt tttcgtaata gcccagatag ctgctggcgc
     7021 cgggatcgtg gccgccgtgg ccggcatcaa cgacgaccag tccggtaagg gagcgcggga
     7081 tttgcgtatc cattgagccg ggcttcgcag gagaaggggt ataagcggac aagttgcctc
     7141 tgattttagg cacaagcagt tccgaaacat aataagaacc gccaacctgt gttaccgtgc
     7201 ctacagggcc gatggctttg ccgtttacat agatgcgtcc gccgtcatat agaaacagca
     7261 gcacccggtt attggcatcg gtcagctcgt aataaggcat gccggctttc tggacggaaa
     7321 gccccaattt agcggccagc tgttcaatgc tgaggcagta ggcgggtaca acggcggctg
     7381 aagtcttccg ctgactgccg ccgcagccga ccaagctgat gcagcctaag atgcatactg
     7441 ccgcgagtaa tctttttgtg ttatgctgca caattattcc tgtcgaagat atcctaattt
     7501 tgatttcaaa cgtcttataa gcatcggtta aatcccctca ttctcttgag ttttgtctat
     7561 atagcgggca ggggctgaaa atccaagaga aaaaagcatt tacttcttcg gtttatttcg
     7621 gcatgccgag caaatgcctt cgatgcgaat ctgctggcgg tggatggtat acccttccgg
     7681 caattggagc agggggacag ccacatccgt caaacattga gtctgaagac atcgccggca
     7741 ggtaaaatgc ggatggcatt ggtgcaggga acagtgatga gccagttcaa agtgccatgt
     7801 gcgttcctca acaaacacac ggtggaccag atttttctgc accagattca tcaatgtacg
     7861 atagatagtc gttttgttgg gcggctgccc gccgatatgt tcggcaatct gctcctgcgt
     7921 caggggggca tcggccttca gcattgccac gagaatagca attcgcggct cggtacgccg
     7981 gagattagag gaacgaagca aatcaatggc cttttgtttc atacgacagc gttttgttct
     8041 tttttaccgc atagaataca ggaatggccg atatgggccc cgtcagagcg gacaaacagc
     8101 aggggaaaga tgatatcact gaaacagcag ggcagccaca cagcaataaa taagacgata
     8161 aacataccca gcaggactgt aagactcaga tctgcttggg tgttcatcag catgtgggca
     8221 gatgaagccc aagtgctgat aagtacatgg cttgcgtgcg gaaggtgtgt ggcgggcttg
     8281 aagtaggcca gaagaacgcc caatagggct gccggaaaaa caagatacca ttcctcgata
     8341 aatccgatat gccagcgtgg atgatgctgg tccgtgtgaa tgtgttctga ttgttgtgca
     8401 gattgctccg gcagggcgtt ctgggcggtc gaatgttcat ggactgcagc gtgggtagga
     8461 acagcaacac ccaaaatgct ttcgccaaaa aagggcagaa tgcagtcgct tagggtggct
     8521 acgccgattg aaccaacaac accgattaag aggatcacga gaaaattctt ggctttccgg
     8581 tgaatctcaa ataaagccgc cgtaaccatc gcactgagaa caacatgcaa gggatgaaaa
     8641 accgagaaca ttgcctcggc ctgttcctta aaccagcgct ggccggcaag cataaagatg
     8701 atgcctgtgg ccgcaccgag catggtaaac ggtgagtgat tccgcagttc cttgccgatt
     8761 aatatcagtt tgccgagtat tctcatataa aaagtctcta aatttgtatt tgcaaccaag
     8821 ttgcatttat tgaatatact tatttgcaac ttagttgcaa gaaaaaattt ctttacatct
     8881 cgttaaaaag gcagatggca ggaaacatcc ccccaaatat tagatttatc gataaaaaat
     8941 cagtttttga ccgctacggc aaggatttca acctttgcgc cggccggcag agcggcagct
     9001 tgaaaagccg cccgcgcagg ataaggttcc ttgaagcagg atttgtaaac ctcatttacc
     9061 gccgcaaagt cggttatctc cgtcagaaag acctgtacct gaaccacatt ccccatatcc
     9121 atgtcagcag cggcaaggat agcccgaata ttgtccaatg cctgtctggc ctgctgtgca
     9181 gtgccgccct cgaccagttt gccggtagcg gggtcaagcc ccaactggcc gctgatatag
     9241 agggtgctgc ccgcaagcag cgcctgtgaa tacggcccga tggccttagg ggctttgtcg
     9301 gtctgaatca tttttcgctg ggtcattgtt ttgccctttt aacaggtcag gcttcagcat
     9361 gtaaatcgta cgctgtgcat acaatttttt ttgatttacc ctgcttgctg acggttaaca
     9421 taaaatgcag attcttcgat tattttgagt tttgttaccc tcttttgatt ctgcctattc
     9481 ggtcttttgg gctgcaccga ttgattttta tccgagtata aagagcggcg cggagttttc
     9541 aatgggatat ttgcaatttg gtgaaatatg cctcctgccg gaactaaggt aaaaaaatcg
     9601 cttgccacta attgggaagc actataaaat aaagagctaa tggagacaga aaatgcggat
     9661 tgttgagacc caacagcaga tgtcgtcgat accgaaaggg gcggtcctga ccatcggcaa
     9721 ttttgacggg gttcatcgcg gtcatcaggc gattctttca actgcccgcc ggcttgcccg
     9781 tgaaaagggc ctcgcttttg ctgtgatgac ttttgacccg catccggcgg tggtgctgca
     9841 tccggaacag gtgctgggca ttctgacgcc ccttcccatc aagacactgc tgtttgaggc
     9901 aatgagcgtt gacatcctga ttgtgataaa agacagcatg ggtttgatga atctgtcgcc
     9961 ggaggacttt gtagatcagt ttctgatgaa gaatctggcg ccgtcggcag tggtagaggg
    10021 gccggatttt aatttcggct atggccgcag cggcagcatc catacgttgc gggagctttc
    10081 agccagtcgg ggcttcgagg tggtggaagt gccctttgcc gagtttttta ctgaaaatga
    10141 ccggcggggg gtaaagtgct ccagcacgac aattcggcat cttcttgagg ccggagatgt
    10201 ctggcaggcc tcccagattc tgggccggcc ataccggttg gttggcaaga ccattgccgg
    10261 ccgcggcatc ggaaggcaat tgggatttcc aacagccaat atcgatccga ttaagcaggt
    10321 tattccggcc gaaggggtgt atgcgggtta tgcagtcgtc ggcgattcgc tggaggaagt
    10381 tgcgttcggc gggctgcgcc ggccggcagc tgtcagcatc ggcagggcta aaacatttgt
    10441 cagcgaccat ccgcttctgc tggaagcgca ctttttggaa gacaaggtag aagacctcaa
    10501 aggcaaatgg ctggcgctgg attttatgcg gtttatccgg catcagcagc ggtttgagac
    10561 ccatcagctg ctccaaaggc agattgccaa agactgccaa aaggcgctga atttcctgct
    10621 gtaaagaccg gttattgcgg caggcgcttc aagaaagagg cagataaatg tgtatttggt
    10681 ttcctttcgg gggggtgctg gcaatttcca tatagccgtc gtgctgttcg acagccagct
    10741 tcgacaagac gatctcgaaa ccggttccgc acatattttg acttcgctct gccggttctg
    10801 ccagcgaacg aatctgctca tcggacaggg cagggccgtc atcgctgatg cgcagttcta
    10861 tctgaggggg atttgtcagc aaaagcgtct gaatggaaat gctgccgctg tgtgcgggca
    10921 gattgtccag cgcgtggatc agcagattga caagcgtttc ttccatgcgg tcggcatcca
    10981 tttgcaccgg cgaaatggat ttgtccaagt gcagctggat tttcacagca tgtttgttca
    11041 gcagcggctg gcaggagtgc agggcttttt caatgatctc atttaaatta caaaccgtct
    11101 tctgaatcgg ataatggcgg gtgtatttaa tcaggtcaag cacaaatttc ttaagccggg
    11161 caagattggg tccaaagacc ccccagcttt gctggatgcg gacataatcg tttcgtgcca
    11221 ttgccagttc gataatctcc gcacacccgg acaccacctg aattatattt ttagccagat
    11281 gagcgaggtc ggccacggat ttgccggata aaagcagcgg ctggagcgac tccggcagag
    11341 cagacatcgc agattcgaat tcattgcgct ctttttggat attcggattg atttgttcca
    11401 tcgtcagtcc tcataaaatt gctgttatcg gctgtatcgg aaagaagatg agaggacttg
    11461 acagggaaaa tgaaaaaaag ccagattcca gctaagcaga atctggcttc ggaggagggt
    11521 gatgaaaagt ttggaaagta taatatcaaa gaactggctt ttgtctgtat cgataaaata
    11581 agcaggaatc tttaaaattt tatcggaaaa ccgattaaaa ttaatccaaa aacactcaaa
    11641 aggggcaaac agggctgttt ttcaggattt tataggtcgt gaaaattgcg cttgccaata
    11701 gacggggcgg attgttatac tggcgtgggt ctcttctttt tgtaggaact gctgatggat
    11761 aaaaaagtta cgatatcaga tctgatggaa gccaagcagg cgggcagaaa gattacggcg
    11821 gtaagctgct atgattatac cactgcccgg ctggttgccc aagcagggat tgagatgatc
    11881 cttgttggtg attcggcggc tcaggtcatg ttgggatacg aaacaaccct gccggcaaca
    11941 atggatttta tggttacaat cactgcagca gtcagaaggg cagccccgaa tgtgtgtttg
    12001 gcggcggata tgccgtttct gtcgtatcaa accggcttgg cagatgcggt tcggaatgcg
    12061 gggcggtttt ttcaggaagc cggcgtgcag atagtcaaag tcgaagccgc ttcagcgcat
    12121 ttggatgtag tcaaggccat cagtgattcc ggaatggcgg tgatggcaca tattggaatt
    12181 cggccgcagc agattgccaa actcggccga ctcaaagccg aaggaacaac agcggagctg
    12241 gcgatggaac tggttgatct gtcgcggcag atggttcagg ccggtgccgg catgttgctg
    12301 ctggaaggaa cagcccgtga agtggcaaag attgttgccc aaatcaatcc ggttcctgtt
    12361 atcagctgcg gttcaggccc tgactgcgac ggtcagattt tgattatttc ggatattttg
    12421 ggactcagcg gccaagccaa gccgaagttt gccaaaaatt ttgcccatct ggatgagccg
    12481 attgttcagg ctgttgccgc gtatgcgcag caggttcggc agatgcagta tccttcggat
    12541 gagcattgct atcatatcaa gtccggccaa ttagaaagat taaggcagat gctggacgaa
    12601 tcgacctaaa acaggacttt ttatgcctga cccgactgcc gaaacagcat ctgaaataac
    12661 aataaagccg gtttatcttg cggataaagg tgtgttgcag tcgtattcgt gctatatccg
    12721 cagaatcctg gttgggctgg cagatacggc acggggggcg gttgtggtct gtcccgatca
    12781 cggacaggcc gaaggtgtac tttgtccttc tgttgagcgg attgagcatc cggcgctgcg
    12841 gctgccgata ttctggcatc agaaccgcag actgcttctg gatcggctgc tgcggcaaag
    12901 gccgaccctg ctgcatgcct tttatccggg gcagattcat ttggcccagt ggctggctga
    12961 tgaactggat gtgccttatg tgattacctt tcatcaagcc ccctcgcggt ggatgcgttt
    13021 tgaaaagccg attcgtcacg ctgcatgcat tattgccccg tcggggccga ttgccgagcg
    13081 tctcaagaaa cagtggcccg gtctgaccga gcggattgta tgtgttccgg taggcagctt
    13141 tgcagaggat caatgcagct gtttttcgcg tcccggcaca atcccgagct tggtggcggt
    13201 gcagccgctg gattcgtttg aggttgttaa gcccctgctg aatgcgattc ggcatctggc
    13261 gctggatggg ttggaattga tagcggcaat tatggggcag gggcgtgcgg aaagggatat
    13321 ccgcagacac atccgaagct tggggctgac ctcagttgtt gcactggtgc ccccgatgca
    13381 tccggtgcgg gatgtgttca gcggtgctga tatttacctg catctgcagg acaggggctg
    13441 ctttgatgcc cagctgttgg aggccttggc agtgggattg gcggtggcgg gctgtcggga
    13501 agaaagcagc ggtctgctga tagagggtca gacagcgctg ttttgggatg cgcatgatga
    13561 actgagcata tacagctgcc tgaaaaccat gttgacccag cgggagaaaa cgcggcagct
    13621 ggcacagaat gcgcaggcgt atctgagcga gcatcacagc gtcagccgga tggtcgagcg
    13681 gcttgcagag atctacgccc aagcccagct taataggggc tttccggaac ccacaggcca
    13741 agcagctccg cagagcgcat aagaaacagc ccgcgttttg acaagacacg aagcatgaag
    13801 caaatgtccg gcttattcaa gacattaata gggtggtata ccaagctgtt tgccttctgg
    13861 attctgctgt ttgcggcagt cggttatttt tttcccaagc cgtttgtgtt tttgaacagc
    13921 tgcaaaatgt tcggcccgtg ggcagaccgc tggccggcgg tgtttcaatc gcttcgaagt
    13981 cccaatctgt ggttttttgc cttgacgatg ttcggcattg gggcggtgct gacgataaac
    14041 gattttaaga acattgcaaa gcggccggtg attgtgctga tcggctcgat tgcgcagttt
    14101 acaattatgc cggtcggggc gtatgcgatt gccaaagcgt ttcatctgcc gccgctgctg
    14161 ggggcgggat tgattattgc cggctgtgca ccgggagcaa tgagcagcaa tgtgatgagc
    14221 tatctggccg gagcggatgt ggcgtattcg gtttcgctga cgacggtttc gacgctgctg
    14281 tgcccgcttc tgacgccggc attgaccaag ctactggccg ggacggaatt gccggtttcg
    14341 ttctggaaga tgttctggga aatcatcttt atggtgattg ttccgctgat ggccggattt
    14401 gcggtgcggt atttcttcgg ccgatttgtc gagcggatca aggatgtatt cccggccatt
    14461 tcggtgacat tcattatctt tgtctgcacc ttagtcattg cctccaaccg tgagaaaatg
    14521 cttgatgcaa cgggactgat tgtgctggcg gtgctgctgc tgaacctcgg cggaatggcc
    14581 ggcggatggg tagtaggggc gctgttcggc atgcgctttg accagcggcg gacgctggcc
    14641 atagaagtcg ggatgcagaa cgccggactg ggcacggtct tggctatcca caatctcggc
    14701 cctgaagcgg cgataccaac ggccttcttt gtttttacct gcattattac cgcctcggtt
    14761 atgacggaga tatggaaagg cagaaagaga gctaaaaatg cgttttgaaa ttggagagat
    14821 gggatggcgt atatacatgg gtattcgcag cgggagatgc agcggctgta tgagcaggcc
    14881 gagattttag aagagattct gcatacaggg actttgtttc cggccggcag cagggtcttg
    14941 gaggccggct gcggggtagg tgcacagaca cggctgctgg ttaagcgcaa ccccgatgcg
    15001 gtgtttacct gcattgacat ctccgaaaag tcgctggctg ctgcccggcg gcttaaagag
    15061 caggccggtt ttgaacatgt tagctttcag aaggaagata tacacaatct atcctttgcc
    15121 gatgagacat ttgatcatgt gtttgtctgt tttgttctgg agcatctgga tgcacctgcg
    15181 gcggcgctga aggaattgaa gcgggtgctt aaaaagggcg ggacaattac cattatcgaa
    15241 ggcgaccacg gctcgtgttt ttggcacccg cagacgccgg aatccatagc cgcctggtac
    15301 gggctgatta aagcccagca gaacatcggc catgacccga ctatcggacg ccgcctgacg
    15361 cctcttttaa cggaggccgg atttcggctg caatcgtgcc ggccggcttg gctgtatgca
    15421 gaccggctca accctgccct gcgtgacggg atggtcaatc acatcatcgt gccgatggtg
    15481 cagtcggccg agacgcagat attggcggat aatcttgtgc cccaagatat gtatcagaaa
    15541 ggcatcgcgg atttgctgcg cgtagatgaa gttgaggagg gcactttttt ctatacatgg
    15601 ttcaaggcgg ttgccggaaa gaacggaatg tgatatagta gcctgcctgt tgccatttcc
    15661 gacagtaaag ataaggattt atatgaaaat tgtagcggat cagaatatcc cgtttgtaaa
    15721 agaatgtttt tcatccctag gggaagttgt gctggtcagc gggcggcaga ttaccccggc
    15781 gctgattcgg gatgccgatg ccctgctggt gcgcagcatt accaaggtgg ataaggacct
    15841 gctggccggc agcagggttc agtttgttgc tacggcgacg attggatttg agcatgttga
    15901 ccgtgattat ctgacggcca atgggattgg ttttgcgtca gctccgggct cgaatgccaa
    15961 cagtgtggcc gagtatatca cagcggctct gctggcgctg ggcaggaagt acaaatttcg
    16021 tctggaaggc aagtctatcg gcattgtcgg ggtgggcaat gtcggcagcc gggttgaaaa
    16081 aaaatgccgg gctttgggga tgaaggcagt gctcaatgat ccgccgctgg cgcggcagac
    16141 cggcgatgcc aagtaccgcc cgctggaaga gctttttgat tgcgatttta ttaccctgca
    16201 tacgccgctg accaaagagg gacctgataa gacctaccat ctggctgatg aacggttttt
    16261 cgcttcgctg aaaaacgggg cggttttcct gaatacctca cgcggcaaag tacacgatga
    16321 agccgccttg aaaagcgcca tgaaggctgg ccgactgagg gcggttgtgc tggatgtctg
    16381 ggaaaccgag ccgaacgtgg acagctggct gctgcggtat gtggatattt ccacgccgca
    16441 tatcgccggc tattcctttg acggcaaggt tgtcgggatg ctgatggtct atgaagccct
    16501 gtgcagacat ttcggcctga aaaccgttca tactgccgcc gaatttctgc ctgatccggc
    16561 tgttcctgaa atccgcatta cagagcagca ggatagtgag cagattattc atcatacagt
    16621 ccagcaggtt tatgccatcc atcgggatga tttcaatatg cgggagattc tgctgcagcc
    16681 gaaagaacag cggggggctt tttttgacaa tctgcgcaaa aactatccga ttcggcgcga
    16741 atttcagaat acggcagttc tgctggccga tacgcagagt cctctggccc gcaagctgga
    16801 gggtatcggc tttcggacgg gtgtactacg gtgaagccat tgcctgatat tgtcatttgg
    16861 cccgggggcc ggctggattt ttccggcggc tgtttggtca tggggatatt aaatgtaacc
    16921 cccgattcgt tcagtgacgg cgggcgctat ttggatgccg atgcggcggc ggctcacggc
    16981 atcgaaatgg ccaagcaggg ggcggcgatt atcgatgtgg ggccggaatc tacccggccg
    17041 ggctcgcagc ctgtatccgc tggggagcaa atccgacggg ctgtgccggt tatcgagaaa
    17101 ttggccaagc agattgatat acctattagc atcgatacac gcaatcccga ggttgccgcc
    17161 gctgcggttg aggcgggggc atctatggtc aatgatatta cagc
//
LOCUS       DAOTBO010000060        16990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2803_length_16990_cov_4.956894, whole genome shotgun sequence.
ACCESSION   DAOTBO010000060 DAOTBO010000000
VERSION     DAOTBO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 16990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16990
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2803_length_16990_cov_4.956894"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4164
                     /gene="gltB"
                     /locus_tag="PLE88_08050"
     CDS             <1..4164
                     /gene="gltB"
                     /locus_tag="PLE88_08050"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOM76453.1"
                     /translation="PSCLGPIALSAEPFIEQVFIDGCGLQGQELERKLYLTRKRTERL
                     VYEALGPAAEDFYIVSLSGRTLCYKGMFMAWQLFAYYPDLNDQRFASALAIVHQRYST
                     NTFPNWKLAHPFRCIAHNGEINTLSGNRNCMRMREKRLKCELLGEDLSDLLPVLTPGG
                     SDSASFDNMLELLTMAGRSMPHALMMMIPEAFGAKYHISTDKRAFYEYHASIMEPWDG
                     PAAMVFSDGRLLGATLDRNGLRPCRYTITTDGLVLMASETGVVEFPPEQIRTKGRLQP
                     GKMFLVDTEEGRIITDNEIKSTISRQKPYRRWLDENRIELKGLFDTPTLELGTPETLA
                     ARMRMFGYTREELRVLIKPMAVNAQEPVGSMGTDTPPAVLSDRPRLLYDYFKQLFAQV
                     TNPPIDPLREGLVMSLMSFTGKKLNILEESARHCRQLKLPHPILTNEDMARLRQIVRS
                     DFKVETIDAVFTLDKTDATLGLKGAIDRLIEQAQIAIVQRDASLIIISDKNAGPDKAP
                     IPALLAVSAVHHALIERGLRGEAGLIVETGEAREVQHFCLLCGYGANGINPYLVFEAL
                     YHMHQQRDLPAELEPVQIADNYIAAVKKGILKTMSKMGISTLRSYFNSQLFEAVGISQ
                     TVIDAYFTGTSSRIGGIGLEQIAADICRRHQNAYTEPQPGSLELEFGGDYAFRLTGER
                     HLWNPTTVSRLQHAAVYNDKKAYEEFAKAVNDQSQALCTLRGLFAFTNTHPIPIEEVE
                     PADAIVKRFCTGAMSHGSISREAHECLAVAMNRLGGMSNTGEGGEDPVRYQKEPNGDS
                     KNCAIKQVASGRFGVTIEYLANAREIQIKMAQGAKPGEGGQLPGKKVSSEIARLRHST
                     PGVTLISPPPHHDIYSIEDLAQLIYDLKCANPSAKVSVKLVSEVGVGTIAAGVAKGNA
                     DEVLISGYDGGTGASPWSSIKHAGCPWELGLAETQQVLVMNKLRDRIRVQVDGQIRTG
                     RDVVIGALLGVERFGFGTAALVCLGCTLLRKCHEGACTFGIATQDPELRKRFKGKPEY
                     IINYLRFVAEEVRQWMALLGFRQFHEMVGRTDKLCMQAALEHYKAKGLDYSKIFQPPT
                     ADRPDALYNSRGQANPLKNHRDWELIEQCRPAFDKRQKVRIQMAVRNTDRTIGAILSN
                     RLLQAFGSAGLPEDTIEIECTGSAGQSFGAFLIKGVTLKLIGDSNDYLGKGLSGGRII
                     VQTPPGSPFQAHENIIAGNTLLYGATSGEVFINGMVGERFCVRNSGATAVVEGVGDHG
                     CEYMTGGLVVVLGQTGCNFAAGMSGGIAYVLDELQLFDTLCNLDMVDLESVWKSEDED
                     LLYKLIEKHQRLTQSARARRILEAWPDMVGRFVKVIPIDYRLALERIRAGEERGTDTT
                     PATEEVFHG"
     gene            4157..5578
                     /locus_tag="PLE88_08055"
     CDS             4157..5578
                     /locus_tag="PLE88_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOM76454.1"
                     /translation="MAKIKGFLEYPRQSVPYRPVEERIGDYREIEIPLTPEAIVQQAA
                     RCADCGIPFCHGAGCPLGNLIPEFNELIYSGKWKEACEVLHATNNFPEITGRVCPAPC
                     ETACTLAVNDKPVLIRHIECQIVERGFREGWIVPLVPRSRTGKTVAVVGSGPAGLAAA
                     QQLARAGHKVTVFERENKPGGLLRYGIPDFKLEKWILDRRLKQMSEEGVEFETQVHVG
                     QDISASYLCRRFDAVCLTAGACQPRDLNIPGRGYENIVFAMDFLRMQNKINAGETAVC
                     PSGLHAAGKNVVVIGGGDTGSDCVGTARRQGAKSITQIEILPQPPETRPPDTPWPMWP
                     RTMRTSSSHEEGCRRMWSILTKQFSGTEIRVSRVHCVRVEWKQINGQWKFTEVPGSEF
                     TLDADLVLLALGFVHVEHGPLVTDLGLKLDDRGNIAVTNCQTSNPKVFAAGDAVSGAS
                     LVVRAIHSGRIAANAVDQYLRMR"
     gene            5650..7293
                     /locus_tag="PLE88_08060"
     CDS             5650..7293
                     /locus_tag="PLE88_08060"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOM76455.1"
                     /translation="MSFRLALAQINSTVGDIGHNAQTIAAMAKRAREQAVDVVVFPEL
                     CLCGYPPEDLLHKPGFLQDNIAALEGLLPEIRGLTALVGFAQPDHQQCYNAAAVIRDG
                     VIASIYHKGQLPNYGVFDEKRYFVPGRNPLVIEQGPTRIAVTICEDIWDIRWLDSYLG
                     DYRGHLDLIVNLSASPFYAGKIKDRQAALVRCSRHFNCAAAYCNLVGGQDELVFDGRS
                     MIVTAQGTIAAQAKAFDEDLYIADIEHHAGRPLTIHPISNSFFSDQNDIVEEVWQALV
                     LGTRDYVRKNGFSRVVVGLSGGIDSSLVAAVAVEALGRQNVIGITMPSRFNSAETRSD
                     AQTLASRLGIEFHTIPISDILDIFSRTLGKVDGWSEQGLAYENLQARIRGTILMSYSN
                     QFGWLVLTTGNKSETAVGYSTLYGDTAGGFAVIKDVPKTLVYKLSAYYNDRAGQPIIP
                     ESVITRAPSAELRPNQKDTDSLPDYDLLDAILKNYIELDHSAADLIHNGFPEEQVRRV
                     LRLVDKSEYKRRQCPPGIKITPRAFGKDRRMPITNKYNP"
     gene            7801..10845
                     /locus_tag="PLE88_08065"
     CDS             7801..10845
                     /locus_tag="PLE88_08065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOM76456.1"
                     /translation="MAGERIPAAGEAMINPDFKPWQKSQHIGYKGLRVPSYFSTPGIH
                     PFDQIEWEKRQAKITDETGKVIFEQNDVEVPANWSQLATKVVVSKYFYGDIHQAGQRE
                     NSVKQLVHRVCKAIADRGLKDGYFASEQDGETFYQELTWLCVNQYGAFNSPVWFNVGL
                     YDVYGVKGSEHNYHWDNRLKKAVPCTSSYEYPQASACFILSVDDTMDDIMRLARSEAM
                     LFKHGSGTGTDLSTLRSSREKLSGGGKPSGPLSFMRVYDQIAAVVKSGGKTRRAAKMQ
                     SLMITHPDIREFITCKTEEEKKAWALIDQGYSPDDAYSSIMFQNSNLSVRVTDEFMEA
                     VEKDGIWRTTAVTTGKKLEEMSARELMNLIAEGTRICGDPGLQYHTTINKWHTCPNSG
                     PIHASNPCSEYMFINNSACNLASLNLMKFRNDDGSFNIDRFKQAIRIFIIAQDILVDN
                     GSYPEASITENSHRFRPLGLGYANLGSLIMSLALPYDSDQARAWAAAVTAIMTGTAYA
                     VSAELAAMKGAFEEFEKNKEPMLNVIAMHREHARNIPEMHCPDYLLSAAKDAWDQAFN
                     AGTQHGYRNAQTTVLAPTGTIGFMMDCDTTGIEPDIALVKYKLLAGGGMLKLVNRTVP
                     LALERLGYSPEDIKDICDAIDRDDTIETAEKLRPEHLPVFDCAFKAKNGKRYIHYMAH
                     LKMMAAVQPFLSGAISKTINMPKESTADEIAAAYMEGWKLGLKAVAIYRDGSKRLQPV
                     NTETKTDSKKKKATEETSAPRPFRRRLPETRNSITHKFSVAGHEGYLTVGLYEDGQPG
                     ELFITMAKEGSTVGGLMDVVGTCVSMALQYGVPLITLVDKFRHARFEPAGMTSNKNIP
                     FAKSLIDYIFCWMGCQFIPGYAEKNIPNPPTAPAVENKTVTTAKELVQKTQDLAKKID
                     QAKALQKKTGIPEIRAAGPALQAAKPPLTDPNRFAERIVQTITAVAGAPDGTQASIMS
                     QFNDQFEHFQDDAPACDICGSICVRNGTCYRCFNCGNSMGCS"
     gene            11342..14209
                     /locus_tag="PLE88_08070"
     CDS             11342..14209
                     /locus_tag="PLE88_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HOM76457.1"
                     /translation="MRFRKNLLLYLGLFFIFGQGCILAVSGGVFGQWGADLLVRIESE
                     LRLSGSLYAESLTDRFPSYAWGQGVMFRALAAASKVDSSYLQTVQNMASEFHSRYRCY
                     YNGRWAYNASANNCGDRYYDDNAWIALGYMELYEITGSSTYLSRAQELVAFCMSGENS
                     PTDTPNGGIRWHESNTSGASVCSTAPTCLANLMIYQATGTEQYLTDAVRLYNWLSTSD
                     LRYASGIYHEANQGPLGYQTAVVLQAAVYLYRITCQAGYLAEAQRLAAAMEHEFINKG
                     THALNQTGKWGGHDMTNAYADLYQTDHNRHWLNIASGYLEFLYKHCRDAASGRYPVSW
                     NNTSGSPSAYLIDNASAAMALWRMAGTLGGSEPAYVTVKNRSSSRCFRLYNSSTADNT
                     NLVIYDRQPTDASELFTLTDAGEGYYILRSWSSNKVLQLYSGLTADNTPIVIYPANAL
                     QHVQQWSLIPAGSGYYNIQNRLTGKSLQPYNLGTVNNTSIVAYTTNPTLSAQHWQFEN
                     LAVPTSITLYFSINGGSWIQSQRAILNVGETASLKADAPGAGVWRWDGPKGYTASGNE
                     ITLEHIQAGQAGLYCAVYTNDSGAESYAAFALGIHAPVTLYQHCSYGGWQAKLGLGAY
                     TLADLTAAGVLNNDASSVKVAEGYRVTFYDSDNFQGPSLVKTADDSCFVDDGWNDRIT
                     SLIIEGQPAPTAYWAFDDRAGLTVRDSSSNGFHGQMTNMDQTSWTMGKRCGGLNFDGI
                     DDYVAVPGFTGIKSGQSRTCAAWIKTTQAGGDIISWGTTAPGCKWIVRLNETGGLRTE
                     VAGGYIYGRKRIDDGQWHHIAVVLENDGTADIAEAVLFVDGQPDMPAAAVYCPVNTAD
                     SVPAAIGLYPTVGIRPFKGLIDEVRIYNRALSNTDVRHLYTASVLAGDIVPDGKVNLA
                     DVQRFAEQWLLAGDAASDTNCDGLTDLADFPYLAAEWLTEQ"
     gene            complement(14333..16009)
                     /locus_tag="PLE88_08075"
     CDS             complement(14333..16009)
                     /locus_tag="PLE88_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOM76458.1"
                     /translation="MYAAKSKSVSFNPLTLAGCLLVLTGLFYISATSAADELHPPLYG
                     PVKPLSAGDTLYVFTKGPRSEDNQFIAMQTLQGVSARTRGPRVWIDAGDDTFVNYLAQ
                     TYSIQFDRRYARDFAALLEILKSCTSGRYVLYDMTDRPSLSAATTMAGLLDAVAVDTQ
                     LEAAAKEKGYVLAVDVRGRDCRWVFENYKPQLNSHAIVVHTNNPRQHRSAPYLRDWGP
                     ALQALDWWHNDLSYSRQVYRSMVPCSPVYGWQDPTTSDEGLTIKLHSEAGLFQIPSDW
                     MLNLSVHAAMGPALKDKKFVQKTARIKPVREEGVHYVTFILSDMDNILTEIGTNSFYS
                     QPKFYANPHRGKFPMSWGMAPSLVELSPAGLEMWYKDAAAGDVFVGYCGLGYFYPSTA
                     PAMEIHTRRLAAFMDRADLRTLLLIDRLLPEKNLDGGYYETAKWFTRLQQIRGLFYLE
                     YIQYAPHGGRIFWFDNKPMVTARFDFRQEAFYPAVRSTPQALADSINQLPKNPASPDG
                     YTFVTVHAWSKGLDDVYQTIQLLDKDVRVVNAEEFIEQVRQNLHPLSSPQ"
     gene            complement(16034..>16990)
                     /locus_tag="PLE88_08080"
     CDS             complement(16034..>16990)
                     /locus_tag="PLE88_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76459.1"
                     /translation="VLTLPMAIRLHTWTHNHNSLSVNYGPLTFSLKIKEKYVKLDSTK
                     TAVSDSQWQPGADPAQWPSFEILPDSSWNYGLVLDKNNPAGAFQLIKNKWPDDDFPFT
                     LDAVPLEMKASAKKIPHWGIDQFGLCAPLQDSPVYSDEPVETITLVPMGAARLRISAF
                     PVIGDGPQACRWKPTPPAPQPADYKTSASHVFDELSALCDGIIPASSDDHSIPRFTWW
                     DHCGTKEWVQYDFGQPRAVSEVSVYWFDDTGIGRCRIPQSWRVLYKDQQDWKPVENAS
                     AFETRPDGWNAASFKSVQTASLRLEVQLRPDFSGGILEWKVK"
BASE COUNT         4088 a         4333 c         4647 g         3922 t
ORIGIN      
        1 ccttcgtgtc tggggccaat cgccctgtcg gcagaaccgt ttatcgagca ggtttttatt
       61 gacggctgcg gcctgcaggg gcaggaactc gaacggaagc tgtatttgac ccgcaagcgc
      121 acggaacggc tggtttatga ggcactcggc ccggccgctg aagactttta tattgtgtcg
      181 ctgtcgggca gaaccctctg ctataagggg atgtttatgg cctggcagct gtttgcctat
      241 tacccggacc tcaacgacca gcgttttgca tcggcactgg caattgtgca tcagcgctat
      301 agcacgaata ccttccccaa ctggaaactg gcgcatccgt tccgctgcat tgcccacaac
      361 ggcgaaatca atacgctcag cggcaaccgc aactgcatgc ggatgcgtga aaaacggctc
      421 aaatgcgagc tgctgggcga ggatttaagc gacttgctgc cggtactgac gcccggaggc
      481 agtgattctg cttcgtttga caatatgctt gagctgctga cgatggccgg ccggagcatg
      541 ccgcatgcct tgatgatgat gattccggag gccttcggag ccaaatacca catcagtact
      601 gacaaacggg cattttacga ataccacgcc tcgattatgg agccgtggga cgggccggcg
      661 gctatggttt tcagcgatgg acgtctgctg ggggcaacat tggaccgcaa cggattgcgt
      721 ccctgccggt atacgattac caccgatggt ctggttctta tggccagcga aaccggcgtc
      781 gtggaatttc cgccggagca gattcgcacg aaaggccgtc ttcagccggg gaagatgttt
      841 cttgtggaca ccgaagaagg ccgaattatt acggacaatg aaatcaaaag caccatctcc
      901 cggcagaagc cgtatcggcg gtggcttgac gaaaaccgca tcgagctgaa gggcttgttc
      961 gatacgccga ctctcgaact gggcactccg gaaacgctgg cggcgcggat gcggatgttt
     1021 ggctataccc gcgaggagct gcgcgtgctt atcaagccga tggccgtcaa tgctcaggag
     1081 ccggtcggct ccatgggaac cgatactccc ccggcggtgc tcagcgaccg gccgcgtctg
     1141 ctgtatgatt acttcaagca gctttttgca caggtaacca atccgccgat tgatccgctg
     1201 cgggaaggat tggtcatgag cttaatgagc tttaccggca agaaactcaa tattctcgag
     1261 gagtctgccc gtcattgccg tcagctgaag ctgccgcatc cgattctgac caatgaggat
     1321 atggctcgtc ttcgtcagat cgtccgcagc gatttcaagg ttgagacgat tgatgcggtc
     1381 tttacactgg acaaaacgga tgcaacgctt gggctcaaag gagccattga ccggctgatt
     1441 gagcaggccc agatagccat tgtgcagcgt gatgcatcgc taattattat cagcgacaaa
     1501 aacgccggac cggataaagc cccgataccg gcgctgctgg ctgtttccgc cgttcatcat
     1561 gcgctgatag aacgggggct gcgcggtgaa gccggcctga ttgtggaaac cggtgaagcc
     1621 cgtgaagtgc agcatttctg tctgctgtgc ggctacgggg ccaacggcat caatccctat
     1681 ttagtctttg aagccctgta tcatatgcac cagcagcggg acctgccggc cgaactggaa
     1741 ccggttcaaa tcgccgacaa ttatatcgcc gccgtcaaaa agggcattct caagacaatg
     1801 agcaagatgg gcatctccac gctgcgcagc tatttcaatt cgcagctgtt tgaagctgtc
     1861 ggcatcagcc agacggtgat tgatgcgtat ttcaccggca ccagctcccg catcggcggc
     1921 atcggtctgg aacagattgc agccgatatc tgccggcggc atcagaatgc ctatacggag
     1981 ccgcagccgg gttctcttga actcgagttc gggggcgatt atgccttccg cctgacgggg
     2041 gagcggcatt tgtggaatcc gacaactgtc agccggcttc agcatgcggc ggtttacaac
     2101 gacaaaaaag cctatgaaga atttgccaaa gccgtcaatg accagtccca agcactctgt
     2161 acgctgcggg gactttttgc ctttaccaat acccatccga taccgattga agaagttgaa
     2221 ccggccgatg ccatcgtcaa gcggttctgc acaggcgcga tgagccacgg ttccatcagc
     2281 agggaagcgc acgaatgtct ggctgttgcg atgaaccggc tgggcgggat gagcaatacc
     2341 ggcgaagggg gcgaagatcc ggtgcggtat cagaaggaac ccaacggcga ctcgaaaaac
     2401 tgtgcgatca agcaggttgc cagcggccgc ttcggcgtta ccatcgagta tctggccaac
     2461 gccagagaaa tccaaatcaa aatggctcag ggagccaagc cgggcgaagg cggccagctg
     2521 ccgggcaaaa aggtttcatc ggagattgcc cggctgcggc attccacgcc gggggtgacg
     2581 ctgatttccc ccccgccgca tcatgatatt tattccattg aggatttagc acagcttatt
     2641 tacgacctta agtgtgccaa tccgtcggcc aaggtttctg tcaaactcgt ttcggaggtg
     2701 ggcgtgggga ccattgccgc cggtgtggcc aaaggcaatg cggatgaagt tctcatcagc
     2761 ggctatgacg ggggtacggg agccagtccg tggtcgtcaa tcaagcatgc cggctgtccg
     2821 tgggagctag ggctggccga aacccagcag gttctggtga tgaacaaact gcgcgaccgc
     2881 attcgcgtgc aggtggacgg gcagattcgt acggggcgcg atgtagtaat cggcgccctg
     2941 ctgggagttg aacggtttgg cttcgggaca gcggcactgg tgtgtctggg atgcactctg
     3001 ctgcgcaaat gccacgaggg tgcctgcact ttcggcattg ccacacaaga ccccgaactg
     3061 cgcaagcgct ttaagggcaa acccgaatac atcatcaact acctgcggtt cgtggccgag
     3121 gaagtgcgtc aatggatggc gctgctggga tttcgtcaat ttcatgaaat ggtcggccgg
     3181 acagacaaac tgtgcatgca ggcggccctt gagcactaca aggccaaagg gctggattac
     3241 tccaagattt tccagccgcc cactgccgac cggccggatg cgttatacaa ttccagaggc
     3301 caagcaaatc cgctgaaaaa tcatcgcgac tgggaattga ttgagcaatg ccgccctgcc
     3361 tttgacaaga gacaaaaggt gcgtattcag atggcagtca gaaacaccga tcggaccatc
     3421 ggggccattt tgagcaaccg tcttttgcag gcattcggtt ccgccggact gccggaggat
     3481 accatcgaaa tcgaatgtac cggctcggct ggtcaaagtt tcggggcatt cctgataaaa
     3541 ggcgtaacgc tgaagctgat tggggacagc aacgattatt tgggaaaggg tctcagcggc
     3601 ggccggatta ttgtccaaac gccgcccggc tcgccctttc aggcgcatga aaatattatt
     3661 gccggcaata ccctgcttta cggagcgaca tccggggaag tattcatcaa cgggatggtc
     3721 ggcgagcggt tctgcgtccg caacagcggc gccactgctg tcgtggaagg ggtgggcgat
     3781 cacggatgtg agtatatgac cggcggtctg gttgtggtgc tggggcagac cggatgcaac
     3841 tttgcagccg gaatgagcgg gggcattgcc tatgttctcg atgaacttca gctttttgat
     3901 accttatgca atcttgatat ggtggatctt gaaagtgtgt ggaaatccga ggatgaagac
     3961 ttgctgtaca aactgattga aaaacaccag cgcttgaccc aaagcgcccg ggctcggcgc
     4021 attctggagg cctggccgga tatggtggga cgattcgtca aagtcattcc gattgattac
     4081 cgccttgcgt tggaacgcat ccgggccggc gaagaacgcg ggacggatac cactccggca
     4141 accgaggagg tgttccatgg ctaaaatcaa aggttttctg gaatacccgc gtcagtcggt
     4201 tccgtatcgc cctgttgagg agcgcattgg cgattacaga gaaatcgaaa ttcccttaac
     4261 gccggaagca attgtgcagc aggctgcacg ctgtgccgac tgcggaatcc ccttctgtca
     4321 tggggcgggc tgtccgctgg gcaatcttat ccctgaattc aacgagctga tttattcggg
     4381 gaaatggaaa gaggcctgtg aggttctgca tgcgaccaac aattttcccg aaattaccgg
     4441 tcgggtatgt ccggcacctt gtgaaacagc ctgcacgctg gcggtcaatg acaagcccgt
     4501 tctgattcgc catattgaat gccagattgt cgaacggggc ttccgcgagg gctggattgt
     4561 gccgcttgtt cccagaagcc gcacgggcaa aacagtcgcc gttgtgggtt cggggccggc
     4621 tggtcttgcg gcggctcagc aattggcacg agccggacat aaagttaccg tctttgaacg
     4681 ggaaaacaaa cccggcgggc ttcttcgcta cgggataccc gatttcaagc tggaaaaatg
     4741 gatacttgac cggcgtctga agcaaatgag cgaggaaggg gtggaatttg agacgcaggt
     4801 gcatgtaggg caagatatct cggcctctta tttatgcaga cgtttcgatg cagtgtgtct
     4861 gacagccggc gcctgccagc cgcgggattt gaacattccc ggcaggggct atgaaaacat
     4921 tgtgtttgcg atggattttc tgcggatgca gaacaaaatc aacgccggcg agacggccgt
     4981 ttgccccagc ggtctccatg cggccggcaa gaatgttgtg gttatcggcg gcggcgacac
     5041 aggaagcgac tgtgtaggca ctgcgcggcg gcagggtgct aaatccatca cccaaattga
     5101 aatcctgcct cagccgcccg agacgcgccc gccggatacg ccatggccga tgtggccgcg
     5161 cacgatgcgg accagctcca gccatgaaga gggctgccgg cggatgtgga gcattctgac
     5221 caagcaattc agcggcacag aaatcagagt cagccgtgtt cactgcgtcc gtgtcgaatg
     5281 gaagcagatc aacggccagt ggaaatttac cgaagtgccc ggatccgaat ttacgctgga
     5341 tgccgatttg gtgctgctgg cactcggatt tgtgcacgtt gaacacggtc cgctggtgac
     5401 agatttgggc ttgaaactgg atgaccgcgg caatattgct gttaccaact gtcagaccag
     5461 caatccgaag gtgtttgccg ccggagatgc cgttagcgga gcatcccttg ttgtgcgggc
     5521 tattcactcc gggcgtattg ccgcaaacgc tgttgaccaa tacctgcgta tgcgttagaa
     5581 tgcggtttga atatgttttg cgtgccttgt tttatcggct tacagctgtt tatggtagag
     5641 gatttgtaaa tgtcgttcag actggcgctg gcacagatca attcgacagt cggggatata
     5701 gggcataatg cccaaaccat cgcggccatg gccaagcggg ccagagaaca ggccgtggat
     5761 gttgtggtat ttcccgaatt gtgcctgtgc ggctatccgc cggaagattt gctgcacaag
     5821 ccgggttttc tgcaggataa tattgcggca ttggagggtt tgctgccgga aatacggggg
     5881 ctgactgctc tggtcggttt cgcacagccc gatcatcagc aatgctacaa tgcagccgcc
     5941 gtgattcgtg atggagttat cgcatccatt tatcataagg gccagctgcc caattacggt
     6001 gtttttgacg aaaaacgcta ttttgtcccc ggccggaatc cccttgttat tgagcagggg
     6061 cctacccgca tcgccgttac catctgcgag gacatctggg atattcgctg gttggattca
     6121 tatctgggag actatcgggg gcatctggac cttattgtca atctgtcggc ttcgcccttt
     6181 tatgccggta aaataaagga ccgccaggca gctctggtcc gatgcagcag gcatttcaac
     6241 tgtgcggcgg catactgcaa tctggtcggc ggacaggacg aactggtctt cgacggccgg
     6301 agcatgattg tcacggcaca gggaacgatt gccgctcagg ccaaagcgtt tgacgaagat
     6361 ctgtatattg cggacattga gcatcatgcc ggcaggccgc tgacaattca ccccatcagc
     6421 aactctttct tttcggatca aaacgatatt gtagaggagg tctggcaggc actggtgctg
     6481 ggcacccgtg attatgtccg taaaaatggc ttttcacggg ttgtagtggg attgagcggg
     6541 ggcatcgatt catcgctggt ggcagctgtc gccgttgaag cgctgggacg tcaaaatgtc
     6601 atcggcatca caatgccgtc ccgattcaat tccgctgaaa cgcgcagcga tgcgcagaca
     6661 ctggcaagcc gtttgggcat tgagtttcac acaataccga tttcggacat actggacata
     6721 ttcagccgca cattgggcaa ggttgacggc tggagcgaac agggactggc ctacgagaac
     6781 ctccaagccc gcattcgggg taccattctg atgtcttact ccaaccagtt cggctggctg
     6841 gttctgacca ccggcaacaa aagcgaaaca gcggtgggct attcgacgct ttacggcgat
     6901 acagccggcg gctttgcggt tatcaaagat gtgccaaaga cgctggttta caagctcagt
     6961 gcctattaca acgatcgtgc gggccagccg atcatcccgg aatcggttat cacccgtgct
     7021 ccttctgcag aattgcgtcc gaaccagaaa gacacagatt cgctgcctga ctatgatctg
     7081 ctggatgcga ttctgaaaaa ctatattgag ctggatcact ctgcggctga tctgattcac
     7141 aacggctttc ccgaagagca ggttcggcgt gtgctgcggc tggtggacaa aagcgaatac
     7201 aagcgccgcc agtgtccgcc gggtattaaa atcacacccc gtgcctttgg caaagaccgc
     7261 cgaatgccta tcacgaataa atataatcca taagactcct gccggcagca gtccctaaat
     7321 ttgtcaatgg agcagtgtta gtgttttgac cttgcattcc tttgcatagc cgagtgtttc
     7381 ctaacatcaa tatacgccct tccctgcggg tcagaacccc cctcttatat gtgctgatat
     7441 ggtcttttgg gagggcttaa ttctattttg gactccatat ttccaatata ttctgtatcc
     7501 gtaaataccc agccggacag agcaaaaaca atccagccaa atcctaaaat ctcaagtgaa
     7561 tattggctgt ttcaagcaat taaaaacgcc ccctttaaat ggttcgataa cagcagaaaa
     7621 aaattttttt tctgttagtt ccgtatttac aagcattaac agacagcgtg caattttttt
     7681 cgatttttac cctttgcaca gcgacctgtc aacggtatat tgacgtagta agtattgact
     7741 atatattgtt atttttattt attatcattc aacatattgt attattcagg gaggtttaca
     7801 atggccggcg aacggattcc agcagcgggt gaagcaatga ttaatccaga cttcaaacca
     7861 tggcaaaaat cacaacatat aggatataaa ggacttaggg ttccgtcgta tttttcaacc
     7921 ccggggattc accctttcga ccaaatcgaa tgggagaaaa ggcaggccaa aatcaccgat
     7981 gaaacgggca aggttatatt tgagcagaat gatgtagaag tccctgccaa ttggagtcaa
     8041 ttggcgacaa aagttgtcgt cagcaaatac ttttatggtg atattcatca agccggccag
     8101 cgtgaaaact ccgttaaaca gcttgttcac agggtttgta aagccattgc agacagggga
     8161 ttgaaagacg gctacttcgc cagcgagcag gatggagaga cattttatca agaattaact
     8221 tggctttgcg tcaaccaata tggggcgttt aattcaccgg tttggttcaa tgtggggctt
     8281 tatgatgttt acggggtcaa gggaagcgag cataattatc attgggacaa tcggttaaag
     8341 aaagctgtgc cctgcaccag cagctatgaa tatccgcagg cctcggcgtg ttttattctg
     8401 tcggttgatg atacgatgga tgacattatg cgtctggccc gcagtgaagc gatgctgttt
     8461 aagcatggtt cgggtacagg tacagacctt tcgacccttc gcagcagccg ggagaagctg
     8521 tccggtgggg gcaaaccttc tggaccgctg agttttatgc gggtttatga ccagattgca
     8581 gccgtagtga aatccggcgg caagacgcgg cgggcagcca aaatgcagtc gctgatgatt
     8641 acgcaccctg acattcggga gtttatcacc tgcaaaacgg aagaagaaaa gaaggcatgg
     8701 gcattaatcg accaaggata cagcccggat gatgcttaca gcagcattat gttccagaac
     8761 tccaatcttt ctgtccgtgt gacagatgag tttatggagg cggttgaaaa agacggcatc
     8821 tggcggacaa cggctgttac taccggcaaa aaactggagg aaatgtcagc ccgtgaactg
     8881 atgaacctga ttgccgaagg aacacgcatc tgcggcgatc cgggcttgca gtatcatacc
     8941 actatcaaca aatggcatac ctgtcccaat tccggaccta tccatgcctc caatccgtgc
     9001 agtgaatata tgttcatcaa caattcggcc tgcaatctgg catcgctgaa cctgatgaag
     9061 ttccgcaatg atgacggctc cttcaatata gaccgtttca agcaggccat ccggattttt
     9121 attattgctc aagatattct ggtcgacaac ggcagctacc cggaagcatc catcacagag
     9181 aacagccatc gcttccgccc gctggggctg ggatatgcca atctgggcag tctgataatg
     9241 agtctggcat tgccctatga ttcggatcag gcgcgtgcct gggcggcggc tgttacggcc
     9301 attatgaccg gcacggctta tgcggtcagt gctgaattgg cggcgatgaa gggagcgttc
     9361 gaggagtttg agaaaaacaa ggagccgatg ctgaatgtca tcgccatgca tcgcgagcat
     9421 gcccgcaata tccccgaaat gcactgcccc gattacctgc tcagcgccgc caaggatgca
     9481 tgggatcagg cgttcaatgc cggaacccag cacggctatc gcaatgccca aacgactgta
     9541 ttggcgccga ccggcactat cggattcatg atggactgcg acacaaccgg cattgagccg
     9601 gatattgctt tggttaaata caaactgctg gccggcggcg gcatgctgaa gctggtcaac
     9661 cgcacagtgc ctctggctct cgagcggctg ggctacagcc cggaagatat caaagatatt
     9721 tgcgatgcca tcgatcggga tgacaccatt gaaacagccg agaagctgcg tccggaacat
     9781 ctgcccgttt ttgactgtgc gttcaaagcc aaaaacggca agcggtatat tcattatatg
     9841 gcccatctga agatgatggc tgccgttcag ccgtttttgt ccggcgctat cagcaagacc
     9901 atcaatatgc ccaaagaaag cactgccgat gaaattgcgg cggcctatat ggaaggatgg
     9961 aagctggggc tcaaggcggt agccatttac cgcgatggtt ccaagcgtct tcagccggtt
    10021 aataccgaaa ccaaaaccga cagcaaaaag aaaaaggcaa ctgaagaaac atccgcgccg
    10081 cgtccgttcc gccggcgcct gcccgaaacc cgcaacagca tcacgcataa attttccgta
    10141 gcaggacatg aaggttatct gacggtcggg ctgtatgaag acggccagcc cggcgaactg
    10201 ttcattacga tggccaaaga aggcagtacc gtcggcggcc tgatggatgt ggtgggcacc
    10261 tgcgtatcga tggccctgca atacggcgtg ccgctgatta cgctggtcga taaattccgc
    10321 catgcacggt ttgagccggc cggcatgacc agcaataaga acatcccgtt tgccaaaagc
    10381 ttgatagact atatcttctg ctggatgggc tgccagttca ttccggggta cgccgaaaag
    10441 aatatcccca accctccaac agcaccggct gttgaaaata aaaccgtcac aacagccaaa
    10501 gagctggtgc agaaaacaca ggatctagct aaaaagattg atcaagccaa agcgcttcag
    10561 aagaaaacgg gaatccctga aatacgtgca gccggtccgg ctctgcaggc agccaaaccg
    10621 ccgctgaccg atccgaaccg ctttgccgag cgcattgtac agacaattac cgctgtcgca
    10681 ggcgcacccg acggcaccca agccagcatt atgagtcagt tcaatgacca atttgaacat
    10741 tttcaggatg atgcgcctgc ttgcgacatt tgcggcagta tctgtgttcg gaacggcacc
    10801 tgctaccgct gcttcaattg cggcaactcg atgggatgct cctgatacaa aagggctttg
    10861 aatcctcgtc agaccgacct tggtacgcta ataccgtatc aggcttgggc acagttttat
    10921 taaacttggt ccagattaaa gccaaggtat cctgcaggat atcctctgca tcagccggac
    10981 atataacaag actgaaaata taaacatata actttctctg gtcggctcaa accagattgt
    11041 tgaaccgttt tatcttttct gtgtctttca tatttttaga aaagttcctt cctctcttct
    11101 tccaaagtaa gaagatcgtc accctgctaa taagcaaatt tctgaaaaat ctaaaccaat
    11161 tttggacccg gtaaaaaaca gaagactttg ttgaattttt tagggcaagc cggaaaacga
    11221 gattttggat ataatacagt ttcaatagac ataaaattta atgaaaaaac gaaatttttc
    11281 gtctaatttt ttaattcata agaaatatat gtatagacta tcgaaccatt gagggggtat
    11341 tttgcgattt agaaaaaact tactgctgta tttaggccta ttcttcattt ttggtcaagg
    11401 ctgcatcctg gccgtcagcg gaggcgtctt tggacaatgg ggggctgatt tgctggttcg
    11461 aattgagtcg gaattgcgtc tatccggcag tctctatgca gaatctctga ccgatcgctt
    11521 tccgagctat gcttgggggc aaggcgttat gttcagagcc cttgcagccg cttctaaagt
    11581 tgactcgtcc tatcttcaaa ctgtacaaaa tatggccagc gagttccaca gccgctatag
    11641 atgctattac aacggccggt gggcctacaa tgccagcgcc aataattgcg gcgaccgcta
    11701 ttacgatgac aatgcatgga tcgcattggg ctatatggaa ttgtatgaaa ttaccggcag
    11761 cagtacttat ttaagtcggg cacaggagct ggttgcgttc tgtatgagcg gcgaaaacag
    11821 ccccacagat acccccaacg gggggattcg ctggcacgag tcaaatacca gcggtgcatc
    11881 ggtctgctcg acagccccaa cgtgtttggc aaacttaatg atttatcagg ccacaggcac
    11941 tgaacaatat ctgacggatg ccgtccgctt atacaattgg ctgtccacat cggaccttcg
    12001 gtatgcatca gggatttatc atgaggccaa tcagggacca cttggatatc aaacagcggt
    12061 tgttcttcaa gctgccgtgt acctctaccg gataacctgc caagccgggt atcttgcgga
    12121 agcacagcgt ctggcggcgg caatggagca tgagtttatc aataagggca cacatgctct
    12181 gaatcagacg ggcaaatggg gcggccatga tatgaccaat gcatatgccg atttgtatca
    12241 gacagaccat aatcggcact ggctgaacat tgcatcaggg tatcttgaat ttctttacaa
    12301 gcattgtcgg gatgcggctt ccggccggta tccggtaagc tggaataaca caagcggctc
    12361 gccttcggct taccttatcg acaacgcctc tgcggcgatg gccttatgga gaatggccgg
    12421 cactttaggc ggttcggaac cggcttatgt aacggtaaaa aatcgttcct catcccgctg
    12481 tttccggctg tataactcat cgacagcgga caataccaat ctggttatct acgaccgaca
    12541 gccgaccgat gcttccgaat tgtttaccct cacggatgca ggcgagggct attatattct
    12601 gcgaagctgg tcttccaata aagtccttca gctctattcc ggactgactg cagacaacac
    12661 ccctattgtg atctatccgg ccaatgccct gcagcatgtc cagcaatggt cgttaatccc
    12721 tgccggcagc gggtattata atatccagaa ccgattgacc ggcaaatcgc tgcagcccta
    12781 caatctggga acggttaata ataccagtat cgtcgcttat acaaccaatc ccaccctttc
    12841 tgcgcagcat tggcagtttg agaatcttgc ggtgccaacg tctattacac tgtatttctc
    12901 catcaacggt ggttcctgga ttcaatctca aagggctatt ctgaacgtcg gagaaactgc
    12961 ttctttgaag gcagacgccc ccggagccgg cgtttggcga tgggacggcc ccaagggata
    13021 cacggcaagc ggcaatgaga tcacgctgga acatattcag gccggacagg cagggcttta
    13081 ttgcgctgta tatactaatg acagcggcgc tgaaagctat gcagcatttg cactgggtat
    13141 ccatgcgccc gttacgctgt atcagcactg cagctacggc ggatggcagg ccaaattggg
    13201 gctcggcgcc tacacactgg ctgacttaac ggccgccggc gtgctgaaca acgatgcttc
    13261 ttctgttaaa gtagctgaag gataccgggt caccttctat gacagcgaca atttccaagg
    13321 gccttcgctg gtaaaaacgg ccgatgattc ctgttttgtg gatgacggct ggaatgaccg
    13381 cataacatcc ctgattatcg aaggccagcc ggctccaacg gcctattggg cgtttgacga
    13441 ccgagccggc ctgaccgtga gggattcttc atccaacggc ttccatgggc agatgacaaa
    13501 tatggatcag acttcttgga cgatgggcaa acgctgcggg gggctgaatt ttgacggcat
    13561 tgatgattat gtggctgtgc ccggttttac cggcatcaag agcggccaaa gccgaacctg
    13621 tgcggcctgg ataaaaacaa cacaggccgg cggtgacatc atcagctggg ggactacggc
    13681 acccgggtgc aaatggattg tgcgcctcaa tgaaacaggc ggccttcgaa cagaagttgc
    13741 cggcggctat atttacggcc gaaaacggat tgacgatggt cagtggcatc atattgctgt
    13801 tgttctggaa aatgacggta ctgccgatat tgcggaagcg gttttgtttg ttgatggtca
    13861 gccggatatg ccggctgcgg ctgtgtactg tccggtaaac acggcggatt cggttccagc
    13921 ggctatcggg ctttatccga ccgtggggat acgccccttt aaagggctta tcgatgaggt
    13981 gcgaatatac aaccgtgcat taagcaacac agatgttcga catctttaca ccgccagtgt
    14041 gctggccggc gatattgtcc cggacggcaa agtcaatctg gccgatgtgc agagatttgc
    14101 ggaacaatgg cttctggctg gcgatgcagc atctgataca aactgtgacg gcttaaccga
    14161 tctggcagat tttccttatc tggctgcgga atggctgaca gagcagtagc tatttggcgg
    14221 gcatccacac tatggactgc aagctcggca gccatttaca cacaacgttt gaggcaggcg
    14281 tccgaagctg ctgccgtgtc tgaaatcaaa catgtgcatt tcgagctgca atttactgcg
    14341 gcgatgagag agggtggaga ttctgacgca cctgttcgat aaactcttct gcattcacca
    14401 ctcttacatc tttgtccaga agctgaattg tttgataaac atcgtccagc cccttgctcc
    14461 aggcatgaac ggtcacaaag gtgtatccat cggggctggc gggatttttg ggcaattgat
    14521 tgatgctgtc ggccaacgct tggggagtgg atcgtacagc cggatagaat gcctcctgac
    14581 gaaaatcgaa tctggcggtt accatcggct tgttatcaaa ccagaaaatt ctgccgccgt
    14641 gcggagcata ctggatgtat tccagataga aaagtccccg aatctgctga agtcgagtaa
    14701 accactttgc cgtttcataa tagccgccgt caaggttctt ttccggcagc agccgatcaa
    14761 tcagcagaag cgtccgcaaa tcggccctat ccataaacgc tgccagccgc cgggtatgaa
    14821 tctccatagc aggagccgtg ctgggataaa aatatcccaa tccgcaataa ccgacaaata
    14881 catcgccggc agcagcatct ttataccaca tctccaaacc agccggcgac agctccacca
    14941 atgacggcgc catgccccag ctcatcggaa acttgccgcg gtgaggattg gcatagaact
    15001 tcggctgcga ataaaaggag tttgtcccga tttcagtcag gatattgtcc atatcgctga
    15061 gaataaatgt aacataatgc acaccctctt ctcggacagg ttttatacgg gcggttttct
    15121 ggacaaactt cttatctttg agagccggtc ccattgcagc atgaaccgac agattcagca
    15181 tccagtcgct gggaatctgg aacagccctg cttcggaatg gagcttgatg gtaagtccct
    15241 catcgctggt tgtcgggtcc tgccatccat acaccggtga acagggaacc atgctgcggt
    15301 acacctgccg tgaataagac aggtcgttat gccaccagtc cagtgcctga agggcaggtc
    15361 cccagtcccg cagatatggt gcgctgcggt gctgccgcgg attgttcgtg tgcaccacaa
    15421 tcgcatggct attcagctgg ggcttgtagt tctcaaagac ccagcggcag tccctgcccc
    15481 tgacatcgac tgccagcaca tagccctttt ctttggccgc cgcttccagc tgcgtatcaa
    15541 cagccaccgc atccaaaagt ccggccatag tcgttgctgc actgagggag ggtcgatcgg
    15601 tcatatcata aaggacatag cgtccgctgg tgcaggattt aaggatctcc agcagcgcgg
    15661 caaaatctct ggcatagcgg cggtcaaatt ggatgctgta ggtctgtgcc agataattga
    15721 cgaatgtgtc atctcccgca tcgatccaca cacgaggtcc ccgggttcga gccgataccc
    15781 cctgcagtgt ctgcatggca atgaattgat tatcctccga acgggggcct ttggtaaaaa
    15841 catacaatgt atcgccggca gacaagggct tgacaggacc atagagcgga ggatgcaatt
    15901 catcggcagc ggacgtggcg ctgatgtaaa agagccctgt cagaaccagt aagcatccgg
    15961 ctaaagtcag cggattgaaa gatacacttt tggactttgc tgcatacatt cagataatat
    16021 gctcctgttg ggtttatttc actttccatt cgaggatgcc gccggaaaag tcgggacgca
    16081 gctgcacttc aagtcttaac gatgccgtct gcacagactt aaatgaagct gcattccacc
    16141 cgtcgggcct tgtttcaaaa gcggatgcat tttcgaccgg tttccagtcc tgctggtctt
    16201 tatacagaac gcgccaggac tggggaattc ggcatcggcc gattcccgta tcatcaaacc
    16261 agtaaacgga cacttctgat acggcacggg gctggccgaa atcatactgg acccattcct
    16321 tggtgccgca gtgatcccac catgtaaagc gggggatgga atggtcatca gaagaagccg
    16381 gaataatccc gtcacagagg gctgacaact cgtcaaatac atgggatgcg ctggttttgt
    16441 aatcggccgg ctgcggcgcc ggaggcgtcg gcttccaccg gcaggcttgc ggcccatcgc
    16501 cgattacggg gaatgctgaa atccggagcc gtgcggctcc cataggcacc agtgttattg
    16561 tttcaaccgg ctcatcggaa tatacagggc tgtcctgcag cggggcacac aaaccaaatt
    16621 ggtctatgcc ccaatgggga atctttttag cggaagcttt catctcaagc ggaaccgcat
    16681 ccagcgtaaa ggggaaatca tcatcaggcc acttgttttt aatcagctga aaggctccag
    16741 cgggattgtt tttgtcaagc acaaggccgt aattccatga ggaatccgga aggatttcaa
    16801 aagacggcca ttgtgccgga tcggctcccg gctgccactg cgaatccgat acagcggttt
    16861 tggtgctgtc cagtttgaca tacttttcct tgatcttcag cgaaaatgta agcgggccat
    16921 aattgacact gaggctgttg tgattgtgcg tccacgtatg gaggcggatc gccatcggca
    16981 gagtcagaac
//
LOCUS       DAOTBO010000061        16967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2810_length_16967_cov_5.349042, whole genome shotgun sequence.
ACCESSION   DAOTBO010000061 DAOTBO010000000
VERSION     DAOTBO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16967
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_16967_cov_5.349042"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..792
                     /locus_tag="PLE88_08085"
     CDS             328..792
                     /locus_tag="PLE88_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM76460.1"
                     /translation="MSKKTLVRPSGRTKALPKEQPCRKCTGRCCRYFALQIDTPRSWD
                     DYDNIRWYLSHRDVAVFVEKGKWYLNVKNTCRYLSKSDFRCRNYRLRPHICRTYSTDN
                     CDLAGDQYDYQMHFSNDRQMEDYMRVKFGAGVFEKLESKQAACRKSNRKGRL"
     gene            789..2072
                     /gene="hisS"
                     /locus_tag="PLE88_08090"
     CDS             789..2072
                     /gene="hisS"
                     /locus_tag="PLE88_08090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOM76461.1"
                     /translation="MKIQAVKGTRDFFPPQMAVRNFIIDGWKAASRRCGFVEYDGPIF
                     EYLQMYQIKSGQEIVEQLFHFEDRGGRRLAIRPEITPTLARMVNQQINTLPRPIKWFS
                     VPRLCRAERPQKGRLREFFQWNIDIIGVEDVLADAEVIFCALDYLRSTGLTAGDVTAK
                     ISSRRLLAALLTNIGIPEKDLDVLYPILDKKSKIPSEAFEEMLKQAVDAPAVIQTIQR
                     LMEVHTIESIPSLVSLNQEARAALEELSKIFAYLNRMGVGDFCRFDLGIVRGLAYYTG
                     MVFEIHDAAGQLRAICGGGRYDNLLRDFGGPEITATGMGMGDCVLEVLLRQKGLLKDD
                     TVSIQTLDYFVACADAALFNEAVAVAAAIRSKGFSCDYSYKPASLAKQLKQADAMKAA
                     KCIIVGQELSKEGRLIVRDMLTGTQETVLCESFLS"
     gene            2229..2648
                     /gene="rnk"
                     /locus_tag="PLE88_08095"
     CDS             2229..2648
                     /gene="rnk"
                     /locus_tag="PLE88_08095"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOM76462.1"
                     /translation="MARQRAIYITKSDYERLTNLISQYQSRQMEAPKHLEELEAELKR
                     AHIVSPMKIAPDVITMNSTIRLKDIDTEEVFDYRLVYPQDADPEKGWISVLAPIGTAL
                     LGFSVGDDIQWEVPAGQRHLHVQEVLYQPEAAGDYTL"
     gene            2724..3125
                     /locus_tag="PLE88_08100"
     CDS             2724..3125
                     /locus_tag="PLE88_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOM76463.1"
                     /translation="MKNVPEQRTFSQIDYYRLQNILAHEQNPAYRQHLKRMSRLRGLL
                     LQGCVCPCKKVASDLITMNSVLRLQSKRGTSFDITLVYPSKACPKDRKYSVLSGIGLS
                     LIGKRQGDYAAKGMQVIEILYQPESDGKFYV"
     gene            3181..3726
                     /locus_tag="PLE88_08105"
     CDS             3181..3726
                     /locus_tag="PLE88_08105"
                     /inference="COORDINATES: protein motif:HMM:NF038001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HOM76464.1"
                     /translation="MGSGAANIYEQAFKFWLEENHIPFVQINQTKRLSGSTIGVKNFD
                     FLLNPAASSPILAEVKGRTFHGKSLAGLKGLDCWTTLEDVQALSHWQQVFVSETQKAQ
                     AVFVFVFQLKQIDIETDGWPIYDCLERRFLLVAISVHAYLRQMKPRSGRWNTVTLPAA
                     DFRRHAVPAGKLLLTPDDLER"
     gene            3735..4712
                     /gene="bioB"
                     /locus_tag="PLE88_08110"
     CDS             3735..4712
                     /gene="bioB"
                     /locus_tag="PLE88_08110"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOM76465.1"
                     /translation="MNKTIQHYAECILDGQLLQRPDIETLSELSKEHLDDLLYWANQI
                     RKANFGNQVRICSIVPGRLGGCSQDCAFCAQSIHYDTPVDKTPKTLSDEEIIAAAAEA
                     KHKGVPNFGIVYSGRKVSDAELDRLIELTKRIKSQLGLGVCMGLGVLDEASMQRLAAA
                     GVSRFNHNLETSRRHFKDIVTTHCYEDRVKTIQAALKAGLGVCAGGIFGIGETEDDRI
                     DMAVELGRLGVDMVPMNFLHPIPGTPLGNTPALPPREILRIIALYRFILPKTHLKVAG
                     GRVLNLRDMQSWIFYAGCTSILTGNYLTTAGRAVEDDLRMLKDLGLETA"
     gene            4747..5382
                     /gene="rsmD"
                     /locus_tag="PLE88_08115"
     CDS             4747..5382
                     /gene="rsmD"
                     /locus_tag="PLE88_08115"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOM76466.1"
                     /translation="MRIIAGYKKGMKLFPPRGMDTRPITDRVKESLFNILWNRGRIAN
                     CVAADLFCGTGSMGLEALSRGAKWVTFIDGSREVVQILRQNIQKTGFVSQSKAVCANI
                     LKVGAAPTPEFGLYDLVFCDPPYEMSTHCGPATKIGRLMELIDRQVNAGGWVILRTHL
                     RAHVEGRYGGLEKIDQREWGSMKIIFFEKTPHAGGGQLVPLSGRQEDACDV"
     gene            5375..6589
                     /locus_tag="PLE88_08120"
     CDS             5375..6589
                     /locus_tag="PLE88_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOM76467.1"
                     /translation="MSNRQAAYKVVRRLRKEGYQALFAGGCVRDRLLGRPASDYDVVT
                     DAVPDAVIKLFRRTLKIGAKFGVVLVLIDAKQVEVATFRTEGGYQDGRHPDYVAFASA
                     KEDAARRDFTVNGMFYDPIDKKVLDFVNGQSDLQKRILRTIGQPDHRFSEDYLRMLRA
                     VRLAVKLDFSIETGTWEAIKKHAGSIRSISAERIAMEIEQILTHPRRAVGVRLLAESG
                     LVYPIFDQLTPESAEFGAAVLEHLPKAVDFPLALASFLAGLPAKTAAAQAAGLKLSSD
                     RIRHIRFLSEKQGLLSDAQLPLSQLKLLMHQPYFWDLAELEKAIALAKGLSLESLKAN
                     RRRAMELDQREIHPAPLLNGHELMALGIAPGPAVGRLASEMYIAQLEGHIKTKEQARQ
                     WCQQQLAKYLAE"
     gene            complement(6593..6898)
                     /locus_tag="PLE88_08125"
     CDS             complement(6593..6898)
                     /locus_tag="PLE88_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOM76468.1"
                     /translation="MVYKRFTNQELILRDELAIDRTRLANERTFLSYVRTALALGAVG
                     GTLLHLYPDNLYLVITGVFFFAAGLFCFIFGIWKTVSMTKQIQILRKQSDQKTPDNS"
     gene            7297..7563
                     /gene="rpsO"
                     /locus_tag="PLE88_08130"
     CDS             7297..7563
                     /gene="rpsO"
                     /locus_tag="PLE88_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOM76469.1"
                     /translation="MLAKEKKEKVIAEYRTKPGDTGSPEVQIAILTTRINELTEHLKT
                     HIKDHSSRRGLLKMVGNRSSLLKYIKNKDINRYRQIISRLGLRK"
     gene            7657..9759
                     /locus_tag="PLE88_08135"
     CDS             7657..9759
                     /locus_tag="PLE88_08135"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOM76470.1"
                     /translation="MFTIHKSEKEIGGKLLSLETGRVARQAHGAVIAQMGQTMVLATA
                     VAAQPPSEEIDYFPLSVDYREKYSAAGKFPGGFIKREGRPSTKEILTARMIDRPIRPL
                     FPDGYFNEVQILVSVISADQDYDPDVLGMVAASAALSISKIPFQGPLGACRISRVDGK
                     FVIFPTYKERDRSDLNLVLGGHRQAINMIEVDAKEAPEEAVGQAVQEAHEAVLAVCDL
                     IDDLTAKCGQEKEFSLFELDAALMAQLEKETAEPFYQAYQIQRKQQRCAAIDQIIEQA
                     AAKYVEADPPACTRVQLTRMFEEIQRKVVRKLLLEGKRPDGRGYDEIRPIDCQVGVLP
                     RSHGSALFTRGETQALVSCTLGTGRDEQMVDGLLAEYGQRFMLHYNFPPFSVGEVKPI
                     RGPGRREIGHGALAEKALEQAAPSEEEFPYTIKLVSDIMESNGSSSMASVCGGSLALM
                     DAGVPVKSAVAGISIGLVSDGSRYELLTDIVGDEDHFGDMDFKVAGTHKGITAIQLDI
                     KAKGLAHSIMMEALERARKARLKILDIMAQTLAQPRPQLSEYAPKIVTIKVDPEYIGK
                     VIGPSGKTVRSIQDQTGSLIEIEEDGTISISCVGGNGHLLARDMIEAMTTPPQVGRIY
                     RNARVVSVKEFGAFLEISPGIEGLCHISEFSKEYVKNIESVCKVGDIIPVKLIMIDEQ
                     GRFKLSRKAALEELQKKA"
     gene            9886..10125
                     /locus_tag="PLE88_08140"
     CDS             9886..10125
                     /locus_tag="PLE88_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOM76471.1"
                     /translation="MARGIVKWFDPKKGFGFVLNEEGMDVFVHYSSIEKEGFRCLRTG
                     QEVEYEEVRAAQGLRGKAVKIVSVPASEDASAPSQ"
     gene            10185..11540
                     /locus_tag="PLE88_08145"
     CDS             10185..11540
                     /locus_tag="PLE88_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM76472.1"
                     /translation="MKKDAAILIVEPDTSLASKLAAVLKSHGASVRMVRSFQEARASM
                     EEDLPDLVLCATHLEKGNDGIAVLTEARAKNPAMPVILISSTADIDACKEAIKLGAYD
                     YLVKPLDTGELVRVVQQAIPSVPALRGMDDFVFSGVVSRSPAMQSVYRVLRRVAPTTM
                     PVLIEGESGTGKELTARAIHLNSHRRDKGFFPINCAGLAESLLESELFGHVKGAFTGA
                     ASDRKGLFQLADKGTLFLDEIGDMPPTMQAKLLRVLEDGLVIPVGGSAPIKVDTRFIS
                     ATNHDLAKLVEEKKFRQDLYFRIKGVSVTLPPLRQRPQDIPELFGYFLKKTCEELGRD
                     IHRITEPAMRAMMSYDWPGNIRQLGNVVRVMVAMCDNDTLDLQDLPPEISRIRQLQAP
                     SLAGPAYTLDDMTGKSLQEVEREHIRRTLELTGHNRTEAAKILRIGERTLYRKIKEYK
                     L"
     gene            complement(11537..11923)
                     /locus_tag="PLE88_08150"
     CDS             complement(11537..11923)
                     /locus_tag="PLE88_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOM76473.1"
                     /translation="MPETPFPIQQYVIQEHTTAADTHWDLMLEMGPALWTWRLPVRPE
                     QIGNEAVQAQRISDHPLRFLNYEGPVQHGTGYVRISDKGHFRICTQTPDRLTVELSGR
                     VLQGRFTLCSTDKPSLWSLQTETEQR"
     gene            complement(12068..13063)
                     /gene="pta"
                     /locus_tag="PLE88_08155"
     CDS             complement(12068..13063)
                     /gene="pta"
                     /locus_tag="PLE88_08155"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOM76474.1"
                     /translation="MSGFFEQLIQRASLHKKTIVLPEGTDQRTIDAAAEIVQLDFANL
                     VILGQPDQLDKQLRSRGVNPSGIQILNPKTDPRLDQYAEAFYEFRKHKGVTPEQARAV
                     VEDEVYFGTMMIKLGHADGLVAGAIHSTGDTLRPALQIIKPAVGISTVSSMFFMCFPD
                     KVLLFADCGLVEDPTEKQLVDIAVATAGTALQFGMEARVAMLSYSTKGSATGPNAVKM
                     AIAAEKAKEAVASIYGSRVILDGELQFDAAYVPSVAAKKCPGSPLQGNANIFIFPDLG
                     AGNICYKSTQRLAGAEAYGPIVQGLARPVNDLSRGCSAHDIVATVAITAIQAAAS"
     gene            complement(13119..13192)
                     /locus_tag="PLE88_08160"
     tRNA            complement(13119..13192)
                     /locus_tag="PLE88_08160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13156..13158),aa:Arg,seq:acg)
     gene            13569..13659
                     /locus_tag="PLE88_08165"
     tRNA            13569..13659
                     /locus_tag="PLE88_08165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13603..13605,aa:Ser,seq:gct)
     gene            complement(14247..15596)
                     /locus_tag="PLE88_08170"
     CDS             complement(14247..15596)
                     /locus_tag="PLE88_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOM76475.1"
                     /translation="MKISRILLRSTIVAALGGLLFGFDTAVISGTTEALQNVFSLSSQ
                     SLGFTVASALIGTIIGALFAGQPADKYGRRTTLILIGVLYFISAVGSALAWNWYAFLI
                     FRFIGGLAVGGASVVSPMYIAEIAPARMRGRLVAVQQFNIVLGILLAFFSNYIIAALD
                     VQAAWRWMLLVEAFPAAAFFLLLFGNPRSPRWLVAQGKTSEAAEVLKMLGTDTGTIDE
                     EIAAIQTSLNQEQYRIAEPFFSRAYAKPIMLAMMIALFNQLSGINALMYYAPHIFKMA
                     GAGQNAAFLQAVAVGSMNLIFTILAMLIIDQFGRKKLMLAGSIGYILSLGTTAWAFYA
                     YGTEFTPVGSRIVLISLLVFIASHAFGQGAVIWVFISEIFPNRLRARGQALGSFTHWF
                     MAAAVSWTFPLFAEASGGHTFAFYAGMMVLQLFWVIFFMPETKGIPLEDIQKELGIH"
     gene            15653..15874
                     /locus_tag="PLE88_08175"
     CDS             15653..15874
                     /locus_tag="PLE88_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76476.1"
                     /translation="MIILLGLRLVKDKKGLRINIFLPACQLWKQKRPKQYGSIRVVQA
                     AGRRRIAFGRRFLYNTEYGCLYDASEGLL"
     gene            15849..16367
                     /locus_tag="PLE88_08180"
     CDS             15849..16367
                     /locus_tag="PLE88_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76477.1"
                     /translation="MMLLKAYYELLYERLKPQRDACCRRMRIRLTEQVQTHFADQIGQ
                     EQYAAYLEAGMAFFDERLEAYNPIGMQYLFDRQQRRTALELQQQLDWYDSRREFHNLL
                     TLVRQKAQSPMTDQQMQAAAAQIIQQAGAFPDQSILEGYRDCPALEKLPDYILAMAIE
                     DLLGRSPQGFNA"
     gene            16396..16671
                     /locus_tag="PLE88_08185"
     CDS             16396..16671
                     /locus_tag="PLE88_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76478.1"
                     /translation="MVRKLFIRLAGIAMLTALSLLASCLANGENNLAEPFAFVQLCDP
                     QLGFSDYAQDQQSLRQAVEQINRLDADFAVICGDMVDNAVSRSIADF"
     gene            16640..>16967
                     /locus_tag="PLE88_08190"
     CDS             16640..>16967
                     /locus_tag="PLE88_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM76479.1"
                     /translation="MPFPAPLRIFKNIIGGLQKPYYCVPGNHDIGSKPTAESLQAYRK
                     AFGRDYFAVEHKGAAFLFVNTQLWKESLEGESEKHHRWLQERLTKAFDKKLQIFVVGH
                     YPLFIKS"
BASE COUNT         4139 a         4169 c         4687 g         3972 t
ORIGIN      
        1 gttttgtcca attccttctg gacctcgcgg cgcagcacat ccggcgtgtt gtgcaggccc
       61 tgcggcatca gcacaacatc gacgatatta ggacttcggg acgcacacca ataggcctcc
      121 cgctgcatca ctttgcagat gacaaacttc agccgcatgc ggccattgta taagacaggc
      181 caatgaatgc aaattgaaaa tcggatgcag ggcaggtttc ggctgttttt caaggacgcg
      241 ggagaatgtc ttgacgaaag aaacggattc cctataatgc ccagatgata aaagacaagc
      301 acgataaaat cagggattta tacaattgtg agcaaaaaaa ctttagttag gccgtccggc
      361 agaacaaaag cgttacccaa ggagcagccg tgcaggaaat gcaccggccg ttgctgccgt
      421 tattttgcgc tgcagattga tacgccccga agctgggatg attacgacaa catccgctgg
      481 tatctgtcgc atcgggatgt tgccgtattt gtagaaaaag gcaaatggta tctgaatgtt
      541 aaaaacacct gccggtatct ttccaagtcc gattttcgct gccgcaacta ccggctccgc
      601 ccgcatatct gccgcaccta cagcaccgac aactgcgatc ttgccggcga ccagtacgat
      661 taccagatgc atttttccaa tgaccggcag atggaagact atatgcgggt gaaatttggt
      721 gccggtgttt ttgagaagct ggaatccaaa caagccgcct gcagaaaatc aaaccgaaaa
      781 ggacgcttat gaaaatacag gcagtcaagg gcacacggga tttttttccg ccgcagatgg
      841 cagtccgcaa ctttattatc gacggctgga aagcggcctc ccggcgctgc ggttttgtcg
      901 aatacgacgg cccgattttt gaatatctcc agatgtacca aatcaaaagc ggtcaggaga
      961 ttgtcgagca gctgttccat tttgaggatc gcggcggccg ccgtctggcc atccgtcccg
     1021 agattacgcc gactttggcg cggatggtca accagcagat caatacgctg ccgcggccga
     1081 tcaagtggtt ttctgtaccc cgtctgtgcc gagccgagcg tccgcagaaa ggccggcttc
     1141 gggagttttt ccaatggaat atcgatatca tcggcgtcga ggatgtgctg gccgacgcag
     1201 aggtgatatt ctgcgcgttg gactatctcc gctcgactgg attaaccgcc ggcgacgtga
     1261 cggccaaaat ctccagccgc cgccttttgg ccgctttgct gaccaatatc ggcattcctg
     1321 agaaagacct tgatgtgctc tatccgattc ttgataagaa gtccaagatt ccttccgagg
     1381 cctttgaaga aatgctcaag caggccgtgg atgcacccgc tgttatccag acgattcaac
     1441 gtctgatgga agtgcatact atcgaatcga ttccttcttt ggtcagcttg aatcaggagg
     1501 cccgggcagc gctggaagaa ttatcaaaga ttttcgctta tctgaaccgg atgggtgtcg
     1561 gtgatttctg ccgttttgat ttgggtattg tgcgcgggct ggcgtattac accggtatgg
     1621 tttttgaaat ccatgatgct gccggccagc ttcgagccat ctgcgggggc ggccgctacg
     1681 ataaccttct tcgtgatttc ggcgggccgg aaattaccgc aaccggcatg ggaatgggcg
     1741 attgtgttct ggaagtgctt cttcgccaaa aaggtctgtt aaaagatgat acggtcagca
     1801 tccagacgct ggattatttt gtcgcttgtg cagatgctgc cctgtttaac gaggccgtgg
     1861 ctgttgccgc tgctatccgc agcaaaggat tttcctgcga ttacagctac aagcccgctt
     1921 cactggctaa acagctcaag caggccgatg ccatgaaagc cgccaaatgt attattgtcg
     1981 gtcaggaatt gtcaaaagag ggcaggctga ttgtgcggga tatgctaacc ggaacgcaag
     2041 agaccgtttt gtgtgaaagt tttctatcct gaagctatat cggaatgacg tttttcggga
     2101 tttagcttga cagcaagtcg agtatctggg attatataag caatatttat cgacactata
     2161 agctgtaatt gatgtaaatt ccgattcaat gccccggtta gcggcagcag tgggaaaggg
     2221 tagttttcat ggccaggcag cgtgccattt acataacaaa gtcggattac gaacgactga
     2281 cgaacctaat aagtcaatat caaagccgtc agatggaagc ccccaaacac ctcgaagaat
     2341 tggaggccga actgaagcgg gcgcatattg tgtcgccgat gaaaattgct ccggacgtta
     2401 tcacaatgaa ttccacgatt cgtctcaagg atatagatac tgaggaggtc tttgattatc
     2461 gactggttta tccgcaggat gccgacccag aaaaggggtg gatttcggtg ctggccccta
     2521 tcggaacggc cctgttggga ttcagtgtgg gggatgatat acaatgggaa gtcccggccg
     2581 ggcagcggca tctgcatgtg caggaggtgt tgtatcagcc ggaggccgca ggtgattata
     2641 cgctttagga tgggattctt ttgaaactgg ttcaaggact ttcaagtttt ttatctattc
     2701 cggccctgaa acggcgacgt ttgatgaaaa atgtgccgga acagaggacc ttttcccaaa
     2761 tagattatta tcgcctgcag aatattctgg ctcatgagca aaatcccgcc tatcgtcagc
     2821 atcttaagcg catgagccgg ctgcggggtc ttctgctgca gggttgcgtt tgtccctgca
     2881 aaaaagttgc gtccgatttg ataacgatga attccgtatt gcgtcttcaa tcaaagcggg
     2941 ggacttcctt tgatattact ttagtctatc cttccaaagc ctgccccaaa gatcgcaagt
     3001 attccgttct ttcagggata gggctttctc tgattggcaa gcggcagggg gactatgcgg
     3061 caaaagggat gcaggtcatc gagattttat accagccgga aagtgatggc aagttttatg
     3121 tttaatcggc ttcaatccat cgggattttg gaaacttcct gatcaacggc ttccaagact
     3181 atgggttctg gtgccgccaa tatctatgaa caggcattta agttctggct tgaggaaaac
     3241 cacatcccat ttgtccaaat taaccagacc aaacgattgt ccggcagcac cataggggta
     3301 aaaaactttg attttctgct gaatcctgcc gccagcagcc ctattctggc cgaagtgaag
     3361 gggcgaacct ttcacggcaa gtcgctggca ggcctaaaag gccttgactg ctggactaca
     3421 ttggaggatg tgcaggcatt gtcccattgg cagcaggttt ttgtctctga aacacaaaag
     3481 gcgcaggcgg tatttgtttt tgtctttcag ctcaagcaga ttgacattga aaccgacggc
     3541 tggccgatat atgattgctt ggaacgtcgg tttcttctgg tggcaatatc tgtgcatgca
     3601 tatctgcggc aaatgaagcc ccgcagcggc cggtggaata cggttactct gccggcggcg
     3661 gattttcgcc ggcatgctgt gccggcaggt aaactgctgt taactcccga tgatttggaa
     3721 agataaaaaa cagaatgaat aaaacaatac aacactatgc cgaatgcatt ttagacggac
     3781 agttgttgca gcgtcctgac atagagacct tgtcggaatt gtctaaggag catttggatg
     3841 acctgctcta ttgggccaac caaatccgca aagccaattt cggcaatcaa gtcaggatat
     3901 gctctatcgt tccgggcagg ctgggcggat gcagtcagga ttgcgccttc tgtgcccaat
     3961 cgattcatta tgatacccct gtggacaaaa ctcccaaaac gctcagcgac gaggagatta
     4021 tcgctgctgc agcagaggcg aaacacaaag gtgttcccaa ctttggcatt gtctatagcg
     4081 gcaggaaggt tagtgatgcg gaattggacc ggctgataga actgacaaaa cgcatcaagt
     4141 cgcagttggg actgggtgtc tgcatgggac tgggcgtttt ggacgaggcc tctatgcagc
     4201 ggctggcggc tgcgggagtc agtcggttca atcacaatct cgagacatcc cgccggcatt
     4261 ttaaagatat tgtgaccacc cattgttatg aagatcgcgt aaagaccatt caggctgcct
     4321 tgaaggcagg actgggtgtt tgtgcagggg gcattttcgg cattggcgag accgaggatg
     4381 atcgcatcga tatggctgtc gaacttggcc ggctgggagt ggatatggtg ccgatgaatt
     4441 ttctgcatcc gatacccggc acgccgctgg gcaatacccc tgcgctgccg ccgcgtgaaa
     4501 tcctgcggat tatcgccctg taccgcttta tcctgcccaa gacgcactta aaagtcgccg
     4561 gcggacgggt gctgaatctg cgcgatatgc aaagctggat cttttatgcc ggctgcacca
     4621 gcattttgac cggcaactat ctgacgactg ccggccgggc tgtggaagac gatttgcgaa
     4681 tgctcaaaga cctcggattg gaaacggcct gaagccggcg gctccccggc aggcagatgg
     4741 agtgatatgc gaattattgc aggttacaaa aaaggaatga aattgtttcc gcctcgcggg
     4801 atggataccc ggccgataac cgaccgggtt aaggaatccc tgttcaatat tctatggaat
     4861 cggggacgca ttgcgaattg tgtggcagcc gacctgttct gcgggaccgg ttcgatgggc
     4921 ttggaagccc tcagccgcgg tgcaaagtgg gtaacgttta tcgacggcag ccgtgaggtg
     4981 gtgcagatac tcaggcagaa catccagaaa accggcttcg tttcacagtc caaagctgtc
     5041 tgcgccaaca ttctcaaagt gggtgctgct cctactccgg agtttggatt gtatgatttg
     5101 gttttttgcg acccgccgta tgaaatgagc acacattgcg gaccagccac caaaatcggc
     5161 cggctgatgg aactgataga taggcaggtc aatgccggcg gctgggtcat cctgagaacc
     5221 catttgcggg cacacgtaga aggccgctac ggtggtcttg aaaaaatcga ccagcgtgaa
     5281 tggggcagta tgaaaatcat ctttttcgaa aaaaccccgc acgccggcgg cggtcaactc
     5341 gtgccgcttt ctggccggca ggaggatgcc tgcgatgtct aaccggcaag cagcgtacaa
     5401 agtggtgcgt cggctgcgta aagagggcta tcaggccctg tttgccggcg gctgtgtgcg
     5461 ggaccgcctg ctgggcagac ctgccagtga ttatgatgtt gttaccgatg ccgttccgga
     5521 tgcggtgatt aaattattcc gccggacgct taaaatcggt gccaaattcg gagtcgttct
     5581 ggtgcttatc gatgctaagc aggtggaggt ggctaccttc cgcactgaag gcggctatca
     5641 ggacggacgt caccccgact atgttgcatt tgcatcggcc aaagaagatg ctgcaagacg
     5701 tgattttact gtcaacggaa tgttttatga cccaattgac aaaaaagtgc tggattttgt
     5761 caatggtcaa agcgaccttc aaaagcggat tttgcggact attggtcagc cggatcatcg
     5821 gttcagcgag gattatctcc ggatgctccg ggcggtgcgg ctggccgtca agctggactt
     5881 cagcattgaa accggcacat gggaggcgat taaaaaacat gccggcagca tacgaagcat
     5941 cagtgccgaa cggattgcga tggaaatcga acagattctt acgcatccgc ggcgtgctgt
     6001 gggggtaaga ctgcttgccg aatccggatt ggtgtatcca atattcgacc aattaacccc
     6061 ggaatctgcc gagtttggag cggccgttct ggagcatttg cccaaagctg tcgatttccc
     6121 cttagcattg gcatcatttc tggccggttt gcccgcaaag acggccgctg ctcaagcggc
     6181 cggactgaaa ctcagcagcg acagaatccg ccatattcga tttctttctg aaaagcaggg
     6241 gctgctttcc gatgctcaac tgcccttatc gcaattaaag ctcctaatgc accagccgta
     6301 tttctgggat ttggccgagc tggaaaaagc gattgcactg gcaaagggtc tgtcgcttga
     6361 gtctcttaaa gccaaccgcc ggcgggctat ggaactggac cagcgggaaa tacaccctgc
     6421 cccgctgctt aatggccatg aattaatggc attaggcatt gcccccggac cagcggtggg
     6481 gcggttggcc agtgaaatgt atattgccca gcttgaaggc catatcaaaa ctaaagagca
     6541 ggccagacag tggtgtcaac agcagcttgc aaagtatttg gcagagtaaa gactatgaat
     6601 tatccggtgt tttttgatcg gattgcttcc gcaggatttg aatctgtttg gtcatagaga
     6661 ccgttttcca gataccgaaa ataaaacaga acagtccggc ggcaaagaaa aaaacacctg
     6721 taattacaag atacaggttg tccgggtata aatgcagcaa ggtgccgccg actgccccca
     6781 gcgccagtgc ggttcgcaca taggacagaa atgtccgctc attggccaga cgggtgcggt
     6841 caatagccaa ttcatcccga aggataagtt cctgattcgt aaaacgctta tagaccatcg
     6901 tttttgccag ttcaaatgaa cgaatccaat atagaaaact gtcaacgcag tgtaaagctt
     6961 ttttaaaaaa agagtggcaa tttccaaatc ggggatgcag ttgggggcgg gatcctgttt
     7021 tgcaggtatc agttctgcat ttgcagagat ttgtattgtt caccgcgtca atatgacgac
     7081 atgttcactc tattttttgg tacaaaatct ttatctggaa aggttagcat taaatctgca
     7141 aacagggcta aaccaaacaa aagacacaaa atatggagca aaaaagaatt gcaaacttcc
     7201 atttgccggc tataatgaac ggcttgaaaa aaatggaaaa cctattagaa gctgggcatg
     7261 tagcccataa gttgtttgaa aggaacgatt taaagtatgc tggctaaaga aaagaaagaa
     7321 aaagttatcg ccgaatacag aacaaaaccg ggcgatacgg gttctccgga agttcagatt
     7381 gcgattctta caacgcgaat taacgaactg acagaacacc tcaagaccca tatcaaagac
     7441 cactcctcgc gcaggggcct gctgaaaatg gttgggaacc ggtcttctct tcttaaatat
     7501 attaaaaaca aggatatcaa ccggtaccgg caaatcattt cgcggctcgg cttacgcaaa
     7561 tagcggctgt ttgttttcaa gcaaaccttg cctttcatat attactggag ataggcagag
     7621 ggtacgagga actttcaaaa gaaacgaaaa gtggatatgt ttactataca taagtcagaa
     7681 aaggagatag ggggaaaact tctgtctctg gaaacaggtc gggttgcgcg ccaagctcac
     7741 ggcgcggtga ttgctcaaat gggccagaca atggttttgg caacagctgt tgctgctcag
     7801 cccccttcag aagagattga ttatttcccc cttagtgttg actatcgaga aaaatacagt
     7861 gcggcgggga aatttccggg aggatttatc aagcgtgaag ggcgtccgtc gaccaaagaa
     7921 attttaactg cccggatgat tgaccggcct attcgccctc tttttccgga tggctatttc
     7981 aacgaggtgc agattcttgt cagcgtcatc agcgccgatc aggactatga cccggatgtg
     8041 ctggggatgg tggcggccag tgcagcgctg tctatcagca agattccgtt tcaggggccg
     8101 ctgggcgcct gccggatcag ccgggtggat ggaaaatttg tgatttttcc aacctacaaa
     8161 gagcgcgaca gaagcgattt gaatctggta ctgggcgggc atcgccaagc catcaatatg
     8221 attgaggtgg atgccaaaga agcgccggaa gaggcggtgg ggcaggctgt gcaggaggcc
     8281 catgaggcgg tattggcggt ttgcgatttg attgacgacc tgacggccaa atgcgggcag
     8341 gaaaaggagt tctccttatt tgaactggat gcagccctga tggcacagtt ggaaaaggaa
     8401 acggcagagc cgttttatca ggcctaccag attcagcgca agcagcagcg ctgtgccgcg
     8461 atcgatcaga ttattgagca ggcggccgcc aagtatgtgg aggccgaccc gccggcctgt
     8521 acgcgtgtgc agctgacgcg gatgtttgaa gaaatacagc gcaaggtggt ccgcaagctg
     8581 ctgctggagg gcaaacgccc cgatgggcgc ggctatgatg aaattcgtcc tatcgactgt
     8641 caagttggtg tgctgccgcg ttcgcatggg tcagcgctgt ttacacgcgg cgagacgcag
     8701 gcactggttt cctgcacgct gggaaccggc cgcgatgagc agatggtcga cgggcttttg
     8761 gccgaatacg gccagcgctt tatgcttcac tataacttcc ccccgttttc cgtcggcgag
     8821 gtcaagccca ttcgcggccc ggggcgccgg gaaattgggc atggtgcatt ggccgagaaa
     8881 gcccttgagc aggcagcccc ttccgaagag gagtttccct ataccatcaa gctggtctcg
     8941 gacattatgg agtccaacgg ctccagttcc atggcttcgg tatgcggcgg ttcgctggcg
     9001 ctgatggatg ccggcgtgcc ggtcaaaagc gccgtggcgg gaatttccat cggtttggtc
     9061 agcgacggca gccgctatga gctgctgacg gatattgtcg gcgacgaaga ccatttcggg
     9121 gatatggatt tcaaagtggc cggcacacac aaaggcatca cggccattca gctggatatc
     9181 aaggccaagg gcttggccca ttccatcatg atggaagctc tggagcgggc gcggaaagcc
     9241 cgtctgaaaa tactggacat catggcccaa acgctggcac agccgcgtcc gcagctgagc
     9301 gaatatgcgc ccaagattgt aacaataaag gttgatccgg aatacatcgg caaggtgatt
     9361 ggcccgtcgg gcaagaccgt tcgcagcatc caagaccaga ccggttcgct gattgaaatc
     9421 gaagaagacg gaaccatcag catcagctgt gtcggcggca acggccacct gctggcccgg
     9481 gatatgattg aagccatgac cacgcctccg caggtcggcc gcatctaccg gaatgcccgc
     9541 gtcgtgtcgg tcaaggagtt cggtgcattt ttggagatct cccccggcat cgaagggctt
     9601 tgccatatca gcgagttcag caaggaatat gtcaagaata tcgaaagtgt ctgcaaagtc
     9661 ggcgacatta ttcccgtgaa gctgattatg attgatgagc agggacgctt taagctaagc
     9721 cggaaggcgg ctctggaaga actgcagaaa aaagcgtaat cagcagcacc aagccggctg
     9781 gtgaggagtt tttcggctga cgcaagaatt gttcggtgag acagtattta tacaaagggt
     9841 tttgaagcag ccttggttgg gatgcttcaa aattggagat ggaagatggc aagagggatt
     9901 gtgaaatggt ttgatcccaa aaagggattc gggtttgtgc tcaatgagga ggggatggat
     9961 gtgttcgtcc actactcaag catcgaaaag gaaggcttcc ggtgtctgcg aacaggacag
    10021 gaagttgagt acgaggaagt ccgtgccgct caaggcctgc gcggcaaagc tgttaaaatt
    10081 gtgtccgtgc cggcctcaga agatgcatcc gcgccatcac agtaattgag gtctacaagg
    10141 cagtctgccc agcagctgcc ggcgatggga ttgagtgtgt tggaatgaaa aaagatgctg
    10201 ccatactgat agttgaacct gacacatccc tggcatccaa gctggcggcg gtattaaaga
    10261 gccatggggc ctctgtgcgc atggtccgct cctttcagga agcccgggca agcatggagg
    10321 aagacttgcc ggaccttgtg ctgtgcgcga cgcatctgga aaaaggcaat gacggcattg
    10381 ccgtgctgac ggaagcccgc gctaaaaatc cggctatgcc ggtcattctg atcagctcca
    10441 ccgccgatat cgatgcctgc aaagaggcga tcaagctggg ggcctatgat tatctggtca
    10501 agccgctgga taccggcgag ctggtgcgtg tggtccagca ggcaattccg tctgtgccgg
    10561 ccttgcgggg aatggatgat tttgtttttt ccggcgtggt cagccgcagc ccggcaatgc
    10621 agtcggtcta tcgtgttctg cgccgggttg caccgacgac aatgcctgtc ctgattgaag
    10681 gtgaatccgg caccggcaag gaattgacgg cgcgggccat tcatctcaat tcgcacaggc
    10741 gggacaaagg ttttttcccg attaactgtg ccggcttggc agaaagtctg cttgaaagcg
    10801 aactgttcgg gcatgtcaag ggagcgttta ccggtgcagc ctcggaccgc aaagggcttt
    10861 ttcagctggc cgataagggc actctgtttc tggatgaaat cggcgatatg ccgccgacga
    10921 tgcaggccaa actgctgcgg gtgctggaag acgggctggt gattccggtc ggcggctcgg
    10981 cccccatcaa ggtggatacc cgctttatca gtgcaacaaa ccacgatttg gccaaactgg
    11041 tcgaagagaa gaagttccgg caggacctgt attttcgcat caaaggcgtc agcgtcactc
    11101 ttcctcccct gcggcagcgg ccgcaggata ttcctgaact gttcggctat tttctcaaaa
    11161 aaacctgcga ggagttagga cgtgatattc atcggattac cgagccggca atgcgggcga
    11221 tgatgagcta tgactggccg ggcaatatcc gccagctggg caatgttgtg cgggtgatgg
    11281 tggccatgtg cgacaatgat acgctggatt tgcaggacct gccgcccgag atttcgcgta
    11341 ttcgccagct tcaggccccg tcgcttgcag ggcctgccta tacactggat gacatgacag
    11401 gcaaaagtct gcaggaagtg gaacgcgaac atatccgcag gacgctggag ctgacgggtc
    11461 ataaccgcac ggaagcagcc aaaatcctcc gcatcggcga gcggacgctg taccgaaaga
    11521 taaaagaata caaactttaa cgttgttctg tttcggtctg cagcgaccat aacgacggct
    11581 tgtctgtgct gcagagagta aaacggccct gcaaaaccct tcctgaaagt tccacggtca
    11641 gcctatccgg tgtttgcgtg caaatcctga aatgcccctt gtcggatatt cgaacgtatc
    11701 ctgtgccgtg ctgcacgggg ccttcataat ttaaaaaccg caggggatga tcgctgatgc
    11761 gctgggcttg gacagcttcg tttccaatct gttccggccg aaccggcagc cgccacgtcc
    11821 acagcgccgg acccatttcg agcatcaaat cccagtgcgt atccgccgct gtggtatgct
    11881 cctgaatgac atactgttgg attgggaacg gtgtttcggg catatgatcc tcccgctgcc
    11941 tgattggcgg cagcccgttg ctgtcggtgc gctgctgcgt cggcgttttt ttaagttccc
    12001 gcaggtattg cctcctaata gagaaatcaa aaacgctgcc ggcacatcgg gggtgttttt
    12061 cagacagtta tgatgctgct gcctgaatgg cggtaatggc gaccgttgcg acaatgtcgt
    12121 gtgcactgca gccccgcgac aagtcattga caggccgggc cagaccctgc acgatggggc
    12181 cgtatgcttc tgcaccggcc agccgctggg ttgatttgta gcagatgttg ccggcgccga
    12241 ggtccgggaa aatgaaaatg ttggcattgc cttgcagggg gctgccgggg cattttttgg
    12301 cggctaccga cggcacataa gccgcatcaa actgcagttc gccgtccaga atgacccgac
    12361 tgccgtagat gctggctacg gcctctttgg ctttttcggc agcaatggcc attttaacgg
    12421 cattcggacc ggttgcggaa cctttggtcg aatagctcag catggccacg cgtgcttcca
    12481 tgccgaattg cagggcggtg ccggctgtgg caacggcgat atcaaccagc tgcttttcgg
    12541 tgggatcttc cacgaggccg cagtctgcaa aaagaaggac tttgtccgga aagcacataa
    12601 aaaacatact ggaaaccgtg ctgatgccta cggcgggctt gatgatctga agggcaggcc
    12661 gcagcgtatc gccggtggaa tgaatagccc ccgccaccaa tccgtcggca tggccgagct
    12721 tgatcatcat cgtgccgaaa taaacctcat cctcaacgac agcacgggct tgttccggcg
    12781 taaccccttt gtgcttgcga aattcataaa aggcctcggc atactgatcc agccgcgggt
    12841 cggttttggg attgagaatc tggatgccgg atggattaac gccccggctg cgcaattgct
    12901 tgtccagctg gtcgggctgg ccaagaatga ccagattggc aaaatcgagc tgtacgattt
    12961 cggcggccgc atcaatggtc cgctggtctg ttccttccgg cagaacaatt gtttttttgt
    13021 gcagcgaagc acgctgaatc agctgttcaa aaaaaccaga cataatattg ccactttcaa
    13081 aagtaggata taatttggtt ctttaaaaaa aagaaaaccg cactcgacag gactcgaacc
    13141 tgtaaccttt ggttccgtag accaatgctc tatccaattg agctacgagt gcaagtcacg
    13201 gctgtacaaa aacttgcgat atcttcgcgt gcactgctgc cgtcggccga tgcattggag
    13261 gcattatacg gcaaagcggg gcactttatc aagtattttg ccgaatcctc ttgtgcggca
    13321 gcagttttgc ggctctcacc gtggccggaa ttgcgggtac tttacaaaaa ataaattcaa
    13381 tttgcaaaaa aaatcagctc tgatttttaa taatttagcc cagcctgtac cggcaaggac
    13441 tgaatatggt ccagacagga tttttcagct gaacggttaa aattagagga cttggtcctt
    13501 aattttcttg caatgccggc gtattgagtg taaggtaagc agaaatttga cgaatagtaa
    13561 aagatactgg agaggtggcc gagaggctga aggcaacggt ttgctaaacc gtcgtacggg
    13621 caaaagcccg taccgcgggt tcgaatcccg ccctctccga ttttcatcta aataccattt
    13681 tttcgctcaa aacaggattc ttccattcta agctgggatt tctctttgcc tttttcgtct
    13741 atagctaatg tgtttttttc tgattgccgc aggggcaggt tttgcgctgt gggatcttgt
    13801 cgaaaatgcc ggctgcaaca ccggatcgcg cggcagagtt tggctctgat tgcagccctg
    13861 cgattgagcg gcttattttt tgaatttacc cccaaaacgg ctattgtctt aaccagcgaa
    13921 tccgccttaa tcacgcgtcc tgttttcggg gggtgttata tcctctttaa cttctaatac
    13981 tgttgctcta tcatacactt gaccttcccc ccgaaaatag taccagttgt catgggagta
    14041 taaccgaacc gttctttctg tattgaggct gccggaagat ggggaggcta ttggtcagta
    14101 attaacaata tctcgatagc cagttaatat attgttaaca atttactgat gaggaaaatt
    14161 cttcattttc agtgcaattt tccaatgcgg ctgaaatagc ttttaataga aaccggccag
    14221 ccgccagtca gccggcttaa aagaacttaa tggatgccca gctccttttg gatatcttcc
    14281 agcggtatgc ctttggtttc gggcataaag aagataaccc agaacagctg caacaccatc
    14341 attccggcgt aaaaggcaaa tgtatgtccg ccggaggcct ctgcaaagag cgggaatgtc
    14401 cacgagactg ccgccgccat aaaccagtgt gtaaagctgc ccaatgcctg tccgcgggca
    14461 cgaagacgat tggggaaaat ttcactgatg aaaacccaga taactgctcc ctgcccgaat
    14521 gcgtgagagg caatgaacac caaaagactg atcagcacga tgcggctgcc gacgggggtg
    14581 aattctgttc cataggcata aaacgcccac gctgttgtcc ccagactgag aatatatccg
    14641 atggagccgg ccagcatcag ttttttccgt ccgaattggt cgataatcag catggccagt
    14701 atggtaaaaa tcagattcat gcttcccacc gcaacggcct gcagaaaggc ggcgttttgg
    14761 ccggcgccgg ccattttgaa aatatggggg gcataataca tcagcgcatt gatgcctgaa
    14821 agctggttga acagggcaat catcatcgcc agcataattg gtttggcgta tgctcggcta
    14881 aagaacggtt cggctatcct gtactgctcc tgattcagcg aggtttgaat ggcggcaatt
    14941 tcttcatcaa tcgtgccggt atcagtcccc agcattttca agacctctgc ggcttcggaa
    15001 gtttttcctt gggcaacaag ccagcgggga ctgcggggat tgccgaacag aagcaggaaa
    15061 aaggcagcgg ccggaaatgc ctccaccaac agcatccacc gccaagcggc ctggacatcc
    15121 agagcggcta tgatgtagtt ggaaaagaag gccagcagaa tgcccaatac gatgttgaac
    15181 tgctggacgg caaccagccg gccgcgcatg cgggcggggg caatttcagc gatgtacatc
    15241 ggcgatacaa ccgaagcgcc cccgacagcc agcccgccta taaagcggaa tatcagaaac
    15301 gcataccaat tccacgccag cgcgctgccg acagccgaaa taaaatacag tactccgatg
    15361 agtatcagcg ttgtccgccg gccgtacttg tccgccggct ggccggcaaa cagagccccg
    15421 atgatggtac ctatcagggc gctggcgact gtgaacccca gcgactgact ggaaagggaa
    15481 aatacgtttt gaagggcctc tgtcgtgccg gaaatgacgg ctgtatcgaa cccaaacagc
    15541 aatcctccca aagccgctac aatagtgctt cgaagcaaaa tcctgcttat tttcatcttt
    15601 tcttcctatc ttttatgaga acggctgttt ccaaccctcc ggtgcagctg cgatgattat
    15661 tctgctgggc ctccgccttg tcaaggacaa gaagggattg cggataaata ttttcctgcc
    15721 ggcctgccag ctctggaaac agaagcggcc caagcaatac ggctctattc gggtggtgca
    15781 ggccgccggc cgccggcgga ttgctttcgg ccggcggttt ctttataata cagaatatgg
    15841 atgcctctat gatgcttctg aaggcctatt atgagcttct gtacgagcgg ctgaaaccgc
    15901 agcgggacgc ctgctgccgg cgcatgcgta tccggctgac agagcaagtc cagacgcatt
    15961 ttgcagacca gatagggcag gaacaatatg cggcctatct ggaagccggc atggcttttt
    16021 ttgatgagcg tctggaggcc tacaatccca tcggcatgca gtaccttttt gaccgtcagc
    16081 agcggcggac tgcccttgag ctccaacagc agttagactg gtatgacagc cgccgcgagt
    16141 ttcacaactt gctgaccctt gtccggcaga aagcccaatc cccgatgaca gaccagcaga
    16201 tgcaggcagc ggccgcacag atcatccagc aggccggggc cttccccgac cagagcattc
    16261 tggagggcta tcgagactgt ccggctctgg aaaaactgcc ggattatatt ttggcaatgg
    16321 caattgaaga cctgttggga agaagtccgc aaggtttcaa tgcataaaat acccaagcaa
    16381 ttacggagta tgcggatggt ccgaaaactt ttcatccggc tggcgggcat tgcaatgctg
    16441 acggccctta gtctgctggc atcctgtctg gcaaacgggg aaaacaatct ggctgaacct
    16501 tttgcatttg tgcagctatg cgatccgcag ctgggctttt ctgactatgc acaagatcag
    16561 cagtccctca gacaagcggt tgagcagata aaccgccttg atgcagattt tgccgtaatc
    16621 tgcggcgata tggttgacaa tgccgtttcc cgctccattg cggattttta aaaacatcat
    16681 tggcggactt caaaagccgt actattgtgt gcccggcaac cacgacatag gcagcaagcc
    16741 gacagccgaa tcacttcagg cctaccgcaa ggcattcggc agagactatt ttgctgttga
    16801 gcataagggg gcggcatttc tttttgtcaa tacccagctt tggaaagaat ctctggaagg
    16861 cgaatcggaa aaacaccatc ggtggctcca agaaaggttg acgaaggcat ttgataaaaa
    16921 attgcagatt tttgtggtgg gccattatcc actgttcatc aagtccc
//
LOCUS       DAOTBO010000062        16808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2837_length_16808_cov_5.601265, whole genome shotgun sequence.
ACCESSION   DAOTBO010000062 DAOTBO010000000
VERSION     DAOTBO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_16808_cov_5.601265"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLE88_08195"
     CDS             complement(<1..608)
                     /locus_tag="PLE88_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM76480.1"
                     /translation="MREDSRAPKGLSSITSVILAVFCAAWAPAQEESSWPYANETKEQ
                     KDARMAWWRQARFGMFIHWGVYAVPAGTYKGHQVKGIGEWIMNRGKIPVAEYKEYAKQ
                     FNPVKYDPDAWVKLAKQAGMKYIIITSKHHDGFALFDSKVTDWDVVDATPYGKDLLAP
                     LAKACRKHGIKLGFYYSQAQDWCHPGGAASRKLAVKDGWDNP"
     gene            complement(750..822)
                     /locus_tag="PLE88_08200"
     tRNA            complement(750..822)
                     /locus_tag="PLE88_08200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(787..789),aa:Lys,seq:ctt)
     gene            1469..2893
                     /locus_tag="PLE88_08205"
     CDS             1469..2893
                     /locus_tag="PLE88_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOM76481.1"
                     /translation="MTGIHWTILTELLLIAGFLLAVVLIAHILLQKRTPSGTIAWLLI
                     IIFMPYAGVPLYLLLGGRKMRQRVLEKGELELPCDCDIPLDKASPIDRILRTYHLPGA
                     EAGHTVRLCRTGAETLEAMTALLQSARRSIYLATFIYQPDAVGRQIRDILAEKAAAGV
                     DVRLMMDGVGSLHTPLRFFRPLTEAGGKVTVFLPVLHRPFRGRTNLRNHRKITIVDNQ
                     TVMAGGANIGRHYMGPEFAGQWKDLAFVMTGHSVRHWLHIFARDWEFAAKEPLTEEQL
                     KLPEAVHHHPVTDRDCTLPASGVVQVIPSGPDVPGDVLHDALLTMIYSAQQRFWAASP
                     YFVPDDALCQALLLALRRGVDVRLVLPRRSNHILPDIVRGIPLRQIQQAGGLVLFYTP
                     RMMHAKVVLMDNHAAVLGSANLDIRSLLLNYEAAMFVYGRQDIQAVADYLEDLMLHSQ
                     CGIAPASLPRQLGESIVRLASPLL"
     gene            complement(2904..4046)
                     /gene="nspC"
                     /locus_tag="PLE88_08210"
     CDS             complement(2904..4046)
                     /gene="nspC"
                     /locus_tag="PLE88_08210"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOM76482.1"
                     /translation="MPYELDKLPTPCYLIDVGLLKKNLLVLQSVQNRCGCRILLAQKG
                     YAAWSTYGLCRQYLAGAAASSLHEARLAHECFGGEVHLCAPAYRDDEMDAYLAIVDHI
                     VFNSFSQWFRFRDKVLSASRPIACGLRINPCHSEVKTKLYDPCAPGSRLGIVRQQFAG
                     PLDGITGLHFHTLCELNADSLARTLEAVEVQFSDIIRQMEWMNFGGGHHITRADYDID
                     LLCELIAAFYKRYSNIKTVYLEPGEAIALHTGVLVASVLDIVHNQLDIAILDTSASAH
                     MPDVLEMPYRPPLDGAGLPGEKKYTYRLAGPTCLAGDVIGDYSFDRPLAVGDKLVFGD
                     MAHYTMVKNTMFNGINLPDIVLYDPEQHAYKLQRRFTYADFKSRLS"
     gene            complement(4128..4313)
                     /locus_tag="PLE88_08215"
     CDS             complement(4128..4313)
                     /locus_tag="PLE88_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOM76483.1"
                     /translation="MGRGDKRTAKGKRCRGSYGKSRSREKIKARVKAALRPKAAQAPA
                     APAPDAAAGAVQTEATA"
     gene            complement(4593..5435)
                     /locus_tag="PLE88_08220"
     CDS             complement(4593..5435)
                     /locus_tag="PLE88_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOM76484.1"
                     /translation="MEKVFLNGQITDADKASVPITDSSYLYGIGLFETMRAAGGRVFR
                     LADHLERLAGSAARLGIVNRFSNDQIAAAIDQTLKANALTDARLRLELSNGPLDSNGA
                     PSCHLLITAAAFTPYPADYYAKGIRVILTDFRQNPKDPYCGHKTTCYGPRLTALKNAH
                     EKLAAEALWFTTENTLAEGCVSNVFLVRQGILYTPPLTTPVLPGIGRKTVLEIAKGHH
                     ISCVEQPLGINDLLGADEVFLTNVIMEVMPVTAVEAHTVGDGKPGPVTRKLSASYKDI
                     LSLK"
     gene            complement(5478..7196)
                     /locus_tag="PLE88_08225"
     CDS             complement(5478..7196)
                     /locus_tag="PLE88_08225"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOM76485.1"
                     /translation="MANADSINSSERMDFIRQIVADDMASGRWGGRVHTRFPPEPNGY
                     LHIGHAKSICLNYGIAADFGGKFNLRFDDTNPCKEEQEYVDAIIEDVRWLGADWQDRL
                     FFGSDYFQQMYDWAVELIQKGKAYVCDLTPEQVRDYRGSLTEPGRESPYRNRSVEENL
                     DLFRRMKEGEFPDGSRTLRAKIDMAHPNLNMRDPVMYRILHASHHRTGDAWCIYPMYD
                     WAHGLEDSIEGITHSICTLEFENHRPLYDWFIDAINEGRSEPIHHPQQIEFARLNLTY
                     TVMSKRKLLQLVREGYVRGWDDPRMPTICGLRRRGYSPEAIRSFCRVIGVNKFNSTVD
                     FALLEHCLRDHLNAASPRVMAVLNPLKVVLTNYPDGHVEQMEAVNNPEDLSAGTRKVP
                     FSKVLYIERDDFMENPPKKFYRLSPGQEVRLRYAYFITCRQVIKDAAGNIVELHCTYD
                     PATRGGDAPDGRKVKATIHWVSAAHALDAEVRLYEHLFIKENPDEAEEGRTFLDNINP
                     DSLKILTGCKVEPSLKNTKPLDRWQFERLGYFCTDPDTSAGRLVFNKTVGLKDTWAKI
                     QQKQQP"
     gene            7342..8265
                     /gene="cysK"
                     /locus_tag="PLE88_08230"
     CDS             7342..8265
                     /gene="cysK"
                     /locus_tag="PLE88_08230"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOM76486.1"
                     /translation="MQRNEHTILSAIGNTPLVELKRINPNPQVRIMAKLEGSNPGGSV
                     KDRPALYMIDQAEKDGLLTHDKTILEPTSGNTGIALAMIGAAKGYRVTLTLPGCVSSE
                     RQHILEAFGAQVIVTCPKQGTDGAIRKARQMLEANPDKYFMPNQFSNPNNPRAHYETT
                     GPEIWQQTEGDIDIFTAGMGTTGTLMGISRFLKDKKNTIKIVGIEPVPGHTIQGLKNM
                     TEAIVPEILDRSRLDEIISMNDEEAFDMTRRLAVEEGLFAGLSSGANVAGAVRLAKKI
                     KSGTIVTIICDRGDRYLSTMLFRSVCAKCPP"
     gene            complement(8629..10017)
                     /locus_tag="PLE88_08235"
     CDS             complement(8629..10017)
                     /locus_tag="PLE88_08235"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76487.1"
                     /translation="MKRRDFLKQTAFAAGGLFGFPTIVKPSVFAAETKPNDKINIGQI
                     GFGRIARGHDLPETIKHDMCRIVAVADVDSKRARDGKRWIESYYARKTGQPGYVDVKV
                     YGDYREMLADSSIDAVIISTPDHWHAQPAMEAALAGKHIYLQKPASLTIAEGRQMSDV
                     VRRSGCVFQIGSQQRSRSPWPQFKRVCQLVRNGRIGQIKHVQIGLPTDPGCGQEPEMP
                     VPENLNYDMWLGSTPTVYYTEKRVHPQNSYDRPGWLRCEQFGAGMITGWGAHHFDTAH
                     WGMDAELTGPVEVEGQAEFPASGLWDVHGDFDVQARYANGITMHISSKFPNGVRFEGS
                     EGWIFVARGAERVTASDPVSGDPNTAPVLVSDAKLLEPSDKPDAVELYDSPEQHLNWL
                     ECIRSRKETVAPVEVAHRSCSVCLVSHIAMKLRRKLRWDPVKEQFIDDAQANAMLSRP
                     QRKPYGTNYVKA"
     gene            complement(10048..10854)
                     /locus_tag="PLE88_08240"
     CDS             complement(10048..10854)
                     /locus_tag="PLE88_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOM76488.1"
                     /translation="MENLCRFSGQTVAKPAAVTAVGLMLVCSSMGLAAHILIYTKNGT
                     GGSRYVHDNIAASVACLKKICQDNGWTCEATDDASVFTAERIKTFDVLVFSNTNNETF
                     DTDQQKKVFQDYIRAGGGFVGIHSACGSERQWPWFWANLGGKFVRHPPLQPFEVRVID
                     KTHPSTSHLPDVWTWQDECYYMNELNPGIHILLAVDLRTIKDDQKDSYPGRVFGDYFP
                     LAWCQEFDGGRQWYTALGHKIEHYSDEHFVRHLTGGIRWAMQKQTAQTAP"
     gene            complement(10818..11846)
                     /locus_tag="PLE88_08245"
     CDS             complement(10818..11846)
                     /locus_tag="PLE88_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOM76489.1"
                     /translation="MKAMENIWIVVLAAAVVIGLAAVLGRTAAAETEKKTQVEAVQDE
                     TAITLQTGSKKILQYYYAVKDVPAGADALYRRSGFIHPLWSPAGRVLTAIQPRDHYHH
                     YGIWAPWTLTHIEGREVDFWNLAKGQGTVRYAGLESLFAGRQEGGFRVRQEHVDFTAD
                     KAGRVSIAETLEVRARPDTVDGKPVWVVDYISRQKNVLDAAIVLDAYRYGGGLGFRAT
                     PQWTRENCTVLTSEGKTRADADGTRARWCDVSGGFDDGGRAGILFLSHPSNRQHPEPM
                     RVWPPDANGGRGDMFFEFCPIRLEGWTLEPGKEYVLRYRLIVYDGTLSSQLAETLWKT
                     YADSAAKP"
     gene            complement(11843..12886)
                     /locus_tag="PLE88_08250"
     CDS             complement(11843..12886)
                     /locus_tag="PLE88_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOM76490.1"
                     /translation="MNNTQWQLLKTILSGRQVVPVPMGFIIDSPWLPGWAGISVLDYF
                     TNDDLWFNANKKAIETFPEVMFLPGFWAEYGMCTEPSAFGAKCSFYFNELPFAEKVID
                     AIEDIDRLTVPNPKQDGLAPFVLNRLRLNRKKIEAMGHSIRFAVSRGPLNIASFLMGT
                     TEFLMGLRTDTEKIHKLLQIITDYIVQWLGVQKKEFDTIDGILILDDIVGFCGQPDFA
                     EFAQPYLKTIFGCLDVSVRFFHNDADGRVCSRHLAQLGVNLFNFSFLHSLEEMKQWTN
                     NQVVLLGNIPPRDVLALGTCRQIAESVQAALSGLTDTSRLILSCGGGMPDGVSTDNLR
                     AFIKTVQERVKGQ"
     gene            13007..13909
                     /locus_tag="PLE88_08255"
     CDS             13007..13909
                     /locus_tag="PLE88_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOM76491.1"
                     /translation="MNRFVRTKNHPFCPQAAGIFWKGRHYSPVFWHATIHQDRAVGSR
                     TTFTEHIHNFYHIVLYTKGRGWYCKEGRFWPAEPGTCVLIHPGQRHEFITRRQTAVYS
                     ELTFSYEHPEAGSLALSFEQLVRIYTGLELNLQEPASLTAEQTQSLLTLLIQMTDYLN
                     SSRLLSVYYAHRTLEQIFDFLAEHASRRQNEQCINDRAARVRQWIEQHYLEPISMEQA
                     ARIAGVSKSYFFRTFKKAFNTTPLAYQQKLRLEAAKTLLRATSLTCSQAADRAGFKDI
                     FFFHRLFKKYTGQTPRQYRRSFDR"
     gene            14055..14702
                     /locus_tag="PLE88_08260"
     CDS             14055..14702
                     /locus_tag="PLE88_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM76492.1"
                     /translation="MVVRVVLVDDHEIMREGMSALLRKYSQFEVVGQASDGRQAVEVV
                     LELKPDIVIMDVGMPNLNGIEATRRMLAQLPNLKVMALSTHSDGNIVAKMIKAGACGY
                     MLKDSAFEELIEGLNTILEGKTFLCNKVSKVVFSEYVSMITNPNRTLDDGLSSREREV
                     LQMVAEGHTTKEIAEALNLSTKTVDSHREHIMEKLGIRNVAGLTRYAIREGLTTP"
     gene            complement(14765..15967)
                     /locus_tag="PLE88_08265"
     CDS             complement(14765..15967)
                     /locus_tag="PLE88_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOM76493.1"
                     /translation="MKMHRRQFIREVFGLWAAGCAAGRLEAASDKQQEWNKSLAQHRI
                     ASVDVKTVQLRWPRQVGKNSRLDVHGWGPRETVCRITTDKGAAGWGQFLAGPQDVQRI
                     RSAVKDRTAADLFDPAVGILKPELRPLDFALHDLAGVILGVPVYEMLGHHGPAANLCY
                     SGMIYFDDLEPPDNPAGIENVLQNCRQDIEYGHRQLKVKIGRGNRWMDKEAGLARDIE
                     VTQRIAAEFAGIDILVDGNDGFTCDSIIRYLEGIGDIRLFWIEEPFAETAEDYAKLKD
                     WLKSHNKTALTADGEYNPDEARLLELLKAGLVDVALQDICGYGFTAWRKYMTVLKDIG
                     VKASPHAWGSRLKTHYTAHLAAGLGNVLTIEGVTCTSDDVDFGDYPLKDGKLVTSSAA
                     GFGMTLKE"
     gene            complement(16155..>16808)
                     /locus_tag="PLE88_08270"
     CDS             complement(16155..>16808)
                     /locus_tag="PLE88_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOM76494.1"
                     /translation="WDVVNEALNENGTLRSTQWLKIIGQDNPDQQYDHIAKAFEYAHA
                     ADPDVELYYNDYNLETSRAKCDGAVAIVKHLQSKGLRIDGVGIQLHGGLTYPKAEDLE
                     YAITTLAATGVKVMITELDIRTQTRGYRGADVSQVSHQSTEDPQAAAAETQKKLADKY
                     AEIFSILVKHRQDIPRVTFWGVYDATSWIGGSPLLFDRNYQPKQAFDAVINVVKGAP"
BASE COUNT         3732 a         4730 c         4559 g         3787 t
ORIGIN      
        1 tcgggattgt cccagccgtc tttgacagcc agcttgcggg aggccgctcc gccgggatga
       61 caccagtcct gcgcctgcga atagtaaaag cccagtttga tgccgtgctt tcggcaggcc
      121 ttggccagcg gggctagaag gtctttgccg tagggggtgg catcgacgac atcccagtcg
      181 gttactttgg aatcaaacag ggcaaagccg tcgtggtgtt tggaggtaat gataatgtat
      241 ttcatcccgg cctgcttggc cagcttgacc catgcatcgg gatcgtattt gaccggatta
      301 aactgcttgg cgtattcctt gtattcggcg acggggattt tgccgcggtt cataatccac
      361 tcgccgatac ccttgacctg atggcctttg taggtgccgg ccggaactgc atagacgccc
      421 caatggataa acattccgaa gcgggcctgc cgccaccaag ccatgcgggc atccttttgc
      481 tctttggttt cgttggcata cggccagctc gactcttcct gagccggtgc ccatgcggca
      541 caaaacactg ccaaaataac cgacgtgatg gaactgaggc ccttgggtgc tctgctgtcc
      601 tctctcatct gcgtatcctt tcgtttgagg ttacctgtcc agaccctgcc ggagcaacag
      661 cacagggccg gaccgttctt tgcattgtct aacaatgcaa ccgccctcct gcacaacaac
      721 cctcccctgc cggatgaaaa taccgccagt gggcgatcag ggactcgaac ccgggacccg
      781 ctgattaaga gtcagctgct ctaccaactg agctaatcgc cctcgatgat aaaactgcca
      841 ttgtattcag tttctttcaa aaaacaacaa aaaaaatttg ataatctgcc cccagcagca
      901 gggggaaaaa tcagccgata tatccgatgc tgtatctgca caacccgccg ctcgatgcct
      961 tttttgaggg tgtctctgcc aaaaatcctg tgccctgcac ggcggccctt tttatatggt
     1021 gcggcacagt taaggccgcc gggtaccaca agactgtttg gccaaccgca aacttaagca
     1081 gcagagtgct aacaatctat taactgacta tcaagatatt gttaattact gaccaacagc
     1141 atccccatct tccagcaaca acaatccccc ctgatcagcc agccggcaat tccagcaaac
     1201 tgggcctccg gcataggcgc ggctggagtg cctttttcgg cagattgcca aacaactttc
     1261 cagtcctgtt gcagtcaata ccccacgaaa ccggcattgt ttttacccac cggcaagagc
     1321 ttgccgattt tagctaaatc aacacccttt atgaaacagc cgttgacagc agagtgaaat
     1381 tatagaataa tcgacctcat aaatcccgta ggcagatgtc ctgaaatgca tcgggacaat
     1441 tgtttaggac aattccgcag ggacaggaat gacagggatt cattggacga ttctgacaga
     1501 gcttctgctg atcgccggct ttctgctggc ggtggttctg attgcccata ttcttctgca
     1561 gaagcgcacg ccatcgggca caatcgcatg gctgctgatt attatcttta tgccctatgc
     1621 gggggtgccg ctgtatcttc tcttgggcgg acgaaaaatg cggcagcggg ttctggagaa
     1681 gggcgaactg gaactgccct gcgactgtga tatccctctg gacaaggcct cgccgataga
     1741 ccgcatcctg cggacctatc atctgccggg agcggaagcg gggcatacgg tcagactgtg
     1801 ccgcacggga gcggaaacat tggaagccat gacagcgctt ctccaaagcg cccgccggag
     1861 catttatttg gcaaccttta tctatcagcc cgatgccgtc ggccggcaga ttcgggatat
     1921 tttggccgaa aaagcggcag ccggcgtcga tgtgcggctg atgatggacg gcgtcgggtc
     1981 gctgcatacg ccgctgcgct tctttcgccc gctgaccgag gccggcggga aagtgacggt
     2041 gtttctgcct gtgctgcacc ggccgtttcg gggacgcacc aacctgcgca accaccgcaa
     2101 gatcaccatt gtcgacaatc aaacggttat ggccggcggc gccaatatcg ggcggcacta
     2161 tatggggccg gaatttgccg gccaatggaa ggacttggcg tttgtgatga ccggccacag
     2221 cgtgcggcac tggctgcata tctttgcccg cgactgggaa tttgcagcca aagaaccgct
     2281 gacagaagag caattgaagc tgccggaggc ggtgcaccac cacccggtaa ccgaccggga
     2341 ctgcaccctg cctgcctccg gggtcgtgca ggtcatcccg tcggggccgg atgtgcccgg
     2401 cgatgtcctg cacgatgcgc tgctgacgat gatctattcg gcacagcagc gtttctgggc
     2461 ggcatcgccc tattttgtgc ccgacgacgc gctgtgtcag gcactgctgc tggcgctgcg
     2521 gcgcggggtg gatgtgcggc ttgtgctgcc ccgtcggtcc aaccacatcc tgccggatat
     2581 tgtgcggggc atccccctgc ggcagattca gcaggccggc ggactggtgc tgttttatac
     2641 cccccggatg atgcacgcca aagtcgtgct gatggataac cacgcggccg tgctgggttc
     2701 ggccaatctg gacatccgca gtctgctgct caattatgaa gcggccatgt ttgtctatgg
     2761 gcggcaggat atacaggccg tggctgacta tctggaggat ctgatgctgc acagccaatg
     2821 cgggattgcc ccggcctctc tgccccgcca gctgggcgaa agcatcgtgc ggctggcttc
     2881 gccgctgctg taatcaggta taatcaggac agacgggatt tgaaatccgc ataggtgaag
     2941 cggcgctgga gtttgtatgc gtgctgttcg gggtcgtaca gcacgatatc cggcagattg
     3001 atgccgttga acatcgtgtt cttgaccatt gtgtaatggg ccatgtcgcc gaagaccagc
     3061 ttgtcgccga cggccagcgg ccgatcgaag gaatagtcgc cgatgacatc gccggccaga
     3121 caggtgggtc cagccagacg gtaggtgtac ttcttttcac cgggcaatcc ggccccgtcc
     3181 agcggcggac ggtagggcat ttccagcaca tcgggcatat gcgccgaagc gctggtatcc
     3241 aaaatggcga tgtccagctg gttatgtacg atatccaata ccgaggccac cagcacgccg
     3301 gtatggaggg caatcgcttc tcccggctcc agatacactg ttttgatatt cgaatagcgt
     3361 ttataaaaag cggcaatcaa ttcgcacagc aggtcaatat cataatcggc gcgggtaata
     3421 tgatggccgc cgccgaaatt catccactcc atctgccgga taatatcgct gaactggacc
     3481 tcgaccgctt ccagcgtccg cgccagcgag tcggcgttca gctcgcagag cgtatgaaaa
     3541 tgcaggccgg tgatgccgtc cagcggccct gcaaactgct gacggacaat gcccagccgc
     3601 gagcccggcg cacagggatc atagagtttg gttttgactt ccgaatgaca cgggttgatg
     3661 cgcagcccgc aggcaatcgg ccgggaggct gacagcacct tgtctcggaa gcgaaaccac
     3721 tggctgaagg aattgaagac aatatggtca acaattgcca gatacgcatc catctcatcg
     3781 tcgcggtagg ccggagcaca cagatgcacc tcgccgccga agcattcatg ggcaaggcgg
     3841 gcctcatgca gggaactggc cgccgcaccg gccagatact ggcggcacag cccataggtg
     3901 ctccatgcag catagccctt ctgggccagc agaatcctgc agccgcagcg gttttgaacc
     3961 gactggagca ccagaagatt cttcttcagc agccccacgt caatcagata acagggcgtc
     4021 ggcagtttgt ccagttcgta cggcaccaaa accggtctcc tgaaataaat aagtcccgac
     4081 ggcagcagac aagccgtctg ggaacttgag aaaaaacagc aaacagttta ggcggttgct
     4141 tcggtctgga cagcgccggc cgccgcatcc ggtgcagggg ctgccggggc ttgagcagct
     4201 ttgggtctca aggccgcctt gacacgggcc ttgattttct ctctgcttcg cgatttgcca
     4261 taggagccgc ggcagcgttt gcccttggca gttctcttgt cacctcgacc catagtattc
     4321 ctttcttttt caacagttca acctattttt attcccgatg aaagtttgcc attatagccg
     4381 actcggaaga aattgcaagg ccttgttcat tcaaaatcgc gcccgcatcc atcataaaag
     4441 ggaggccgct gggggcgttt tggaatgcct tgcctgcttc tgattccaga ccggtattgc
     4501 ccccttgtgg gggcaagttt tggacgtgca ctggcaagag tttcagcccc gccagctgtc
     4561 aacagcagct ggccagctga cctttgcgat acctatttaa gacttaaaat atctttataa
     4621 gatgctgaca attttctggt gacaggtccg ggtttgccgt cgccgacggt gtgggcttca
     4681 acggcagtga caggcatgac ttccataatc acattggtca aaaagacctc atcagccccc
     4741 agcaggtcat tgatgcccag cggctgctca acacaggaaa tatgatgacc tttggcaatt
     4801 tccaaaacag ttttgcggcc gattccgggc agaacgggcg tggtcagcgg cggggtatag
     4861 aggatgccct gcctgaccaa aaagacattg ctgacacacc cttcggccaa tgtattttcc
     4921 gtagtaaacc acagggcttc ggccgccaat ttctcgtggg catttttcag ggctgtcaga
     4981 cgcggaccat agcaagtggt cttgtggccg caataggggt ctttgggatt ttggcgaaaa
     5041 tcggtcagaa tcacccgtat ccctttggca taataatccg ccggataggg ggtaaatgcg
     5101 gcggctgtaa tcagcagatg acagctcggt gcgccgtttg aatccagagg gccgttgctc
     5161 aattccagcc gcaggcgggc atcggtcaga gcgtttgcct tgagggtctg gtcaattgcg
     5221 gcagctatct ggtcattgga aaaccggttg acgatcccca gacgcgcagc gctgccagcc
     5281 agacgctcca gatgatcggc cagccggaat accctgccgc cggctgcacg catcgtttca
     5341 aacagcccaa tgccgtacag gtagctgctg tccgtaatcg gcaccgaggc tttgtctgca
     5401 tccgttatct gaccgttgag aaataccttt tccatgctat ccgcctgact gtgcagcggc
     5461 gctggtttcc aagcgcctta gggctgctgt ttctgctgga ttttggccca cgtgtctttc
     5521 aggccgacgg ttttgttgaa gaccagccgg ccggcgctgg tatcgggatc ggtgcagaag
     5581 tagcccagcc gctcaaactg ccatcggtcc agcggttttg tgtttttcag cgacggctca
     5641 accttgcagc cggtcaggat tttcagggag tcgggattga tattgtccag aaatgtccgg
     5701 ccctcttcgg cttcgtcggg attttctttg ataaacaaat gttcatacag ccgaacttct
     5761 gcatcgaggg cgtgggcggc actgacccaa tggatagtag ccttgacctt gcggccgtcg
     5821 ggggcatcgc cgccacgagt ggccgggtcg tatgtgcagt gcagctccac gatattgccg
     5881 gcggcatctt tgataacctg ccggcaggta ataaagtagg cgtagcgcag gcgcacttcc
     5941 tgtccggggg acaggcggta gaactttttg ggcgggtttt ccataaaatc atcccgctcg
     6001 atgtagagca ctttggaaaa cggcaccttg cgagtgcccg ccgacaggtc ttcagggttg
     6061 ttgaccgcct ccatctgctc gacgtgtccg tcggggtagt tggtaagaac aactttgagc
     6121 ggattcagca cagccatcac acgggggctg gcggcgttga ggtggtcgcg gaggcaatgc
     6181 tccagcagcg caaaatccac cgtgctgttg aacttgttga cgccgataac ccggcagaag
     6241 ctgcgaatgg cttccggcga atagccccgc cggcgcaggc cgcagatggt cggcatccgg
     6301 gggtcgtccc agccgcggac gtagccttcc ctgaccagct gcagcagctt gcgcttgctc
     6361 ataaccgtat aggtcagatt cagacgggca aattcaatct gctgggggtg atgaatcggt
     6421 tcggaccgcc cttcgttgat ggcatcaata aaccagtcgt acagcgggcg gtgattttca
     6481 aactccagcg tgcagatgga atgcgtgatg ccctcgatgg aatcttccag cccgtgggcc
     6541 cagtcgtaca tcggatagat gcaccatgca tcgccggtgc ggtggtgaga ggcgtggaga
     6601 atgcggtaca tcaccgggtc gcgcatattg aggttggggt gggccatatc gattttggcc
     6661 cgcagggttc gcgagccgtc aggaaactcg ccttccttca tccggcggaa caaatcgaga
     6721 ttttcctcta ccgaacggtt tcgataggga ctttcccggc cgggttcggt cagactgccg
     6781 cggtaatccc gcacctgctc gggcgtcagg tcgcagacat aggccttgcc tttttgaatc
     6841 agctcaaccg cccagtcgta catctgttgg aaatagtcag agccgaagaa caggcggtcc
     6901 tgccagtcgg cgccaagcca gcggacatct tcaataatcg catcgacata ctcctgctct
     6961 tccttgcagg gattcgtatc atcaaagcgc agattgaatt tgccgccgaa atcggccgca
     7021 atgccgtagt tgagacagat gctcttggca tggccgatat ggagatagcc gttgggttcg
     7081 gggggaaaac gggtgtgcac gcgcccgccc cacctgccgc ttgccatatc atcggctact
     7141 atctgccgga taaaatccat ccgctccgaa ctgtttatcg agtctgcatt tgccatcgca
     7201 catccttatc caataggtca atttttccaa aaccaagctt ggaatataaa aaaaacagtc
     7261 gacaatcgca agagaacatt ttagcatatt tatcgcaaaa cacagtcctt taccgttttt
     7321 attctgcggc aggaaatatc gatgcaaagg aatgagcata caattttgtc ggccatcggc
     7381 aatacgccgc tggtggaact gaagcggatc aatccgaacc cgcaggtgcg gattatggcc
     7441 aaactggaag gcagcaatcc cggcggctcg gtcaaagacc ggccggctct gtatatgatt
     7501 gaccaagccg aaaaggacgg cctgctgacc cacgacaaga ccatcctcga gccgaccagc
     7561 ggcaataccg gcattgctct ggccatgatc ggcgctgcca agggctaccg ggtcacgcta
     7621 accctgccag gctgcgtcag cagtgaacga cagcatattc tggaggcctt cggggctcag
     7681 gtgattgtta cctgtcccaa gcagggtacg gatggggcta tccgcaaggc ccggcagatg
     7741 ctggaagcca atcccgacaa atactttatg cccaaccagt tttccaatcc gaataatccc
     7801 cgcgcccatt atgagacaac cggaccggaa atctggcagc aaaccgaagg tgatatcgac
     7861 attttcaccg caggaatggg tacaaccggc acactgatgg gcatatcccg gtttttgaag
     7921 gacaaaaaaa acacgattaa aattgttggt attgagccgg tgcccgggca tacgattcag
     7981 ggcctgaaaa atatgaccga agccattgtc cccgaaatac tggacagaag ccgtctggat
     8041 gaaattatct ccatgaacga tgaggaggca ttcgacatga cccgccggct agctgtggaa
     8101 gaggggcttt ttgccggcct ttccagcggg gcgaatgtgg cgggggctgt ccggttggca
     8161 aagaagataa aatccggcac cattgtaacc attatttgcg accgcggcga ccggtatttg
     8221 agcaccatgc tcttccggtc ggtctgcgcc aaatgtccgc cgtaattgca ggcgtttgca
     8281 atttctgcaa aaaatccaga actgctgctg aacctgacga tagagacaga cgtaagaaca
     8341 atcagtgcag agtggggatc tgcgccaaaa tgtaaaccct tttttgccca tcgatagaca
     8401 gtgggtggag gcaaaaccat gaagcagctt ggcaaagcac gcggacacaa ttacccacgc
     8461 aagcaatccg ttcgtttacc cgttttggtc gtaaagcatt ccgtctttgg acggtctgcc
     8521 agtcgaaaaa caaaataact acccctcttt aagccggatg tgccggcatc cttgcccgac
     8581 ttctgaaaga gccgggcagg gacggccggg catttttaca gagggatttt atgccttgac
     8641 atagttggtt ccatagggct ttcgctgggg acgcgacagc atagcgttcg cctgagcatc
     8701 atcgataaac tgctccttga ccggatccca gcggagtttc cgccgcagct tcattgcaat
     8761 atggctgaca agacacacgc tgcaggagcg gtgggcaacc tccacagggg caacggtttc
     8821 tttgcggctg cggatacatt ccagccagtt gagatgctgc tcggggctgt catacagctc
     8881 gacggcatcg ggcttgtcgg acggctccag cagcttggcg tcgctgacaa ggacaggggc
     8941 ggtgttcggg tcgccggaaa cagggtcgct ggcggtaacc cgctcggcgc cgcgcgccac
     9001 aaaaatccag ccctcgctgc cttcgaaccg caccccgttg ggaaatttgc tgctgatgtg
     9061 catggtgatg ccgttggcat agcgggcctg cacatcgaag tccccatgca catcccacag
     9121 gccggatgca ggaaactcgg cctgcccttc cacttcaacc ggacccgtca gctcggcatc
     9181 cattccccag tgggccgtat cgaaatggtg agctccccag ccggttatca tgccggcgcc
     9241 gaactgctcg cagcgcagcc agccggggcg gtcatagctg ttctgcggat gaacgcgctt
     9301 ttcggtgtaa tagactgtcg gcgtggagcc cagccacata tcataattga ggttttccgg
     9361 cacaggcatt tcaggttctt ggccgcagcc ggggtcggtc ggcagaccga tttggacgtg
     9421 ttttatctgg ccgatgcggc cgttgcggac aagctggcag acccgcttga actgcggcca
     9481 cggactgcgc gagcgctgct ggctgccgat ttgaaaaaca cagccgctgc gccgcaccac
     9541 atcgctcatc tgacggcctt ccgcaatggt cagggaggcg ggcttctgaa gatagatgtg
     9601 cttgccggcc agagccgctt ccattgccgg ctgggcatgc cagtggtcgg gggtggaaat
     9661 gatcaccgca tcgatgctgc tgtcggccag catttcacgg taatcgccgt agaccttgac
     9721 atccacatag ccgggctggc ctgtctttcg ggcgtaataa ctctcaatcc agcgcttgcc
     9781 gtcacgggcc cgtttggaat ccacatccgc cacagccacg atgcggcaca tatcatgctt
     9841 gatggtttcg ggcaaatcat gaccgcgggc aatccgtcca aagccgattt ggccgatgtt
     9901 gattttgtca ttgggtttgg tttcggcggc aaaaaccgac ggtttgacaa ttgtcggaaa
     9961 tccaaaaagg ccgcctgcgg cgaaagccgt ctgcttcaaa aagtcccttc gcttcataca
    10021 tcacctcgtg cttttcagtt aaactggtta tggggctgtc tgtgccgttt gtttctgcat
    10081 agcccagcgg atgccgccgg tcagatgccg gacaaagtgt tcatcgctgt aatgctctat
    10141 cttgtgtccc agcgcggtgt accactgccg gccgccgtca aattcctgac accatgccag
    10201 cggaaaatag tctccgaaaa cgcgtccggg atagctgtct ttctgatcat ccttaatggt
    10261 tcgcaaatcc actgccagca gaatgtgaat acccggattc agttcgttca tatagtagca
    10321 ttcatcctgc catgtccaga catccggcag atggctggtg gaaggatggg ttttgtcgat
    10381 aacccgcacc tcgaacggct gcaggggggg atggcggaca aacttgccgc ccaaattagc
    10441 ccagaaccac ggccactgcc gctcgctgcc gcaggccgaa tgaatgccca caaaccctcc
    10501 gccggcacgg atatagtcct gaaaaacctt tttctgttgg tctgtatcaa aggtttcgtt
    10561 gttggtattg gaaaaaacca gtacatcaaa ggttttgatt ctttcggcag taaagactga
    10621 ggcatcatcg gtcgcttcgc acgtccagcc gttgtcctga caaattttct tcagacatgc
    10681 cacgctggcg gcgatgttgt cgtgcacata gcgcgagccg cctgtgccgt ttttggtata
    10741 aatcagaata tgggctgcca atcccatcga actgcacacc agcatcagcc cgacggcagt
    10801 cactgccgcc ggctttgcta cggtttggcc gctgaatctg cataggtttt ccataaggtc
    10861 tccgcaagtt gagaagagag tgttccatca tagacaatta accggtagcg caggacatat
    10921 tccttgccgg gttccaatgt ccagccttcc agccggatgg ggcagaattc aaagaacata
    10981 tccccccgtc cgccgttggc atcgggcggc cagacgcgca tcggctccgg atgctggcgg
    11041 ttggacgggt ggcttaagaa cagaatgccg gcccgtccgc catcgtcaaa gcccccgctg
    11101 acatcgcacc agcgtgcccg cgtgccgtcg gcatcggcgc gggttttgcc ttcggatgtt
    11161 aagactgtgc agttttcccg tgtccactgc ggggttgccc gaaagcccag accgccgccg
    11221 tagcggtagg catccagcac aatagccgca tccaatacat ttttctggcg gctgatatag
    11281 tccaccaccc agaccggctt gccgtcaaca gtatccggcc gggcgcgaac ttccaatgtt
    11341 tcggcgatgg aaacacgccc cgctttgtcg gctgtaaaat caacatgttc ctgccgcacg
    11401 cgaaatccgc cttcctgccg gccggcaaac agggattcaa gcccagcata gcggaccgtc
    11461 ccctgtcctt tggccagatt ccagaagtcc acttcgcggc cttcgatatg cgtcagcgtc
    11521 cacggcgccc agatgccgta gtggtggtaa tggtctcggg gctgaatagc ggtcagaacc
    11581 cgtccagccg gcgaccagag cggatgaata aaaccgcttc gccgatacag ggcgtctgcc
    11641 cccgcgggaa catctttgac ggcataatag tactgcagga tttttttgct gccggtttgg
    11701 agcgttatgg cagtctcatc ctgcaccgct tccacctgtg tctttttttc cgtctcggct
    11761 gccgccgtcc ggcctaaaac agctgccagc cctatcacca cagccgctgc caagacaaca
    11821 atccagatgt tttccatcgc tttcattgcc ctttaaccct ttcctgaacc gttttgataa
    11881 acgcccgcag attgtccgtg gatacgccgt ccggcattcc gccgccgcag gacagaatca
    11941 gccggctggt gtcggtcagt cccgacaaag ccgcctgtac gctttcggca atctgccggc
    12001 aagtccccaa agccagcaca tcccgcggcg ggatattgcc cagcagaaca acctgattgt
    12061 tggtccactg cttcatttct tcaagcgaat gcaggaagct gaaattgaac agattgacac
    12121 ccagctgtgc caaatgccgg ctgcataccc ggccgtctgc gtcattatgg aagaaccgga
    12181 cggatacatc cagacagccg aagattgtct tcagatacgg ctgggcaaac tcggcaaaat
    12241 ccggctggcc gcaaaagccg acaatatcat ccagaatgag aatgccgtca attgtatcaa
    12301 actctttttt ctggacgcca agccattgta ctatgtaatc agtgatgatt tgaagcaatt
    12361 tgtgtatttt ttccgtatcg gtccgcaaac ccatcaggaa ctcggtggtc cccatcagaa
    12421 aggaggcaat gttcaacgga ccgcgcgaca cagcgaaccg aatcgaatgc cccatggctt
    12481 cgattttttt acggttcaga cggagccggt tgagtacaaa cggagccaag ccgtcctgtt
    12541 taggatttgg cacagtcagc cggtcaatat cctcaatcgc atcaataacc ttttcggcaa
    12601 aaggcagctc attgaaataa aaactgcact tggcaccaaa ggcgctgggc tcggtgcaca
    12661 ttccgtattc ggcccagaag cccggcagaa acatcacttc cgggaatgtt tctatggctt
    12721 ttttattggc attaaaccag aggtcatcat tggtaaaata atccaataca gaaatgcctg
    12781 cccatcccgg cagccatggg ctgtcgataa taaatcccat cggcacagga acaacctgcc
    12841 ggccggaaag aatggttttc aaaagctgcc actgcgtatt attcatcgtc ttgtcctatt
    12901 actgcaaaca caccagcttg ccgtgctatt gcatcattgc attatagtac tcatttatat
    12961 agaatgaaat ggaaaatagt gctgttttat tgcataataa tgaactatga atcgatttgt
    13021 ccgaacaaaa aatcatccct tttgcccgca ggcggcaggg attttctgga agggcaggca
    13081 ttacagccct gtcttttggc atgcgacgat tcatcaggac agagccgtcg gcagccgaac
    13141 caccttcact gagcatattc acaatttcta ccatattgtg ctttacacaa aggggcgagg
    13201 ctggtactgc aaagagggcc gcttttggcc ggctgaaccg gggacgtgcg ttctgattca
    13261 tccggggcag cggcatgagt tcatcacccg ccggcagaca gcggtatatt cagaactgac
    13321 attttcctat gaacacccgg aagccggctc gctggcgtta tcgtttgagc agcttgtgcg
    13381 gatctatacc ggtctggagc tgaacctgca ggagccggcc agtctgacgg ctgaacaaac
    13441 ccaaagtctg ctgacccttt tgatacaaat gaccgattat ctcaacagca gccggctttt
    13501 gtctgtctat tatgcccacc gcacactgga acaaatcttt gactttctgg ccgaacacgc
    13561 cagccggcgg caaaatgaac aatgcatcaa tgatcgagct gcacgcgtgc ggcagtggat
    13621 tgagcagcac tatctggaac cgatttcaat ggaacaggcc gcccgcatcg ccggagtttc
    13681 gaaaagctac tttttccgga cttttaaaaa ggccttcaac accacgccgc tggcctatca
    13741 gcagaagctg cgtcttgaag cagccaagac gcttctgcgg gctacctctc ttacatgcag
    13801 tcaggctgcc gaccgtgccg gctttaaaga tatattcttt tttcatcgct tatttaagaa
    13861 atacaccggc caaacacccc gccaatatcg gcggtctttt gacagataaa aaatggacag
    13921 attttatctg aaacaaaacg cttccttcgg aaggtccgaa aatccttatt gaactttaca
    13981 atggggtatt gtaaaatcat ttttatgaag acataaaaca gatagggaat gtcgcagatc
    14041 tggagaaaag ggccatggtt gtaagggtag tgttagttga tgatcatgag ataatgcgag
    14101 aggggatgag tgcgcttctg cgcaaatact cccaatttga agtggtcggc caagcttcgg
    14161 acggccggca ggcagtcgaa gtggtgctgg aactcaagcc ggacatcgtt attatggatg
    14221 tgggtatgcc gaatctcaac ggtattgaag ctacccgccg gatgctggca cagcttccca
    14281 atcttaaagt aatggctttg tctacccatt cggacggcaa tatcgtggcc aagatgatta
    14341 aagccggcgc ctgcggctac atgctcaaag actcagcctt tgaagagctg attgagggtc
    14401 tcaacactat tttggaaggc aagacatttc tgtgcaacaa ggtttcaaag gtagtctttt
    14461 ccgagtatgt cagcatgatt accaatccca accggaccct cgacgatgga ctcagcagcc
    14521 gggagcgcga ggttcttcaa atggttgctg aaggccacac aaccaaggaa atcgccgaag
    14581 ccctgaattt gagcaccaaa accgtcgatt cccatcgcga acacattatg gaaaaactgg
    14641 gcatccgaaa tgttgcgggc ctgacccgct atgccattcg cgaagggctg acgaccccgt
    14701 aagaggtacg attgcggagc tgtcgcaggc agccggcaac cgtgccgccg gtctacaaag
    14761 gccgttattc tttcagcgtc atcccaaaac cggcagcaga agatgtaacc agtttgccgt
    14821 ctttgagcgg ataatcgccg aagtccacat catcgctggt gcaggtaacg ccttctatcg
    14881 taagcacatt gcccaaaccg gcggccaaat gggccgtata atgcgtcttc aggcgcgaac
    14941 cccatgcgtg cggcgaggcc ttgacgccga tgtctttgag caccgtcata tactttcgcc
    15001 atgcggtaaa accgtagccg cagatatcct gcaaggcaac atcgaccaat cctgccttta
    15061 gaagctccag cagccgggct tcgtccggat tgtactcgcc gtctgcggtc agggcggtct
    15121 tgttgtgcga tttcagccag tctttaagtt ttgcataatc ctccgctgtt tccgcgaacg
    15181 gctcctcaat ccagaacagc cggatatcgc cgattccttc cagataccgg ataatggaat
    15241 cgcacgtaaa gccatcattg ccgtccacaa gaatatctat gccggcaaac tcggcggcga
    15301 tccgctgggt gacctctata tcccgcgcca gacctgcctc tttgtccatc catcggttgc
    15361 cgcgtccgat tttcaccttg agctggcggt ggccgtattc gatatcctgc cggcagttct
    15421 ggagcacgtt ttcaatgccg gccgggttgt ccggcggctc caaatcatca aaataaatca
    15481 ttccgctgta gcacaaattc gcagcggggc cgtgatggcc cagcatctca tacacgggaa
    15541 cacccaaaat gacccctgca aggtcgtgga gggcaaaatc cagcggccgc aattcgggtt
    15601 tgagaatgcc aactgccgga tcaaacaagt cggctgctgt cctgtctttg acggctgacc
    15661 gaatgcgctg gacatcctgc gggcctgcca gaaactgccc ccagccggcg gcgcctttgt
    15721 ccgtggtgat gcggcagacc gtttcccgcg gtccccagcc gtgtacatca agacgcgaat
    15781 ttttaccgac ctgccgcggc cagcgaagct gaaccgtctt gacatcaaca gaagcgatgc
    15841 ggtgctgcgc gagactcttg ttccattcct gctgtttgtc ggaggcggcc tctaaacggc
    15901 ctgcggcgca gccggccgcc cataatccaa aaacctcccg tataaactgc cgccgatgca
    15961 ttttcatcct gaccccgctt tcctgaatat ccagccgact gcaccatcat ccgctttccg
    16021 ctgccttgca gcgggttagc tgtctgcggg aacatagtat cccttcccct gccgctgcgg
    16081 caaggacttt tttcacaaca gcacaaaatt gcaccggttt cccgccattg ggcacagacc
    16141 cggcggttcg ggttctaagg ggcgcctttt accacattga tgactgcatc aaaggcctgt
    16201 ttgggctgat agtttctgtc aaacagcaga ggcgaaccgc caatccacga ggtggcatcg
    16261 taaacgcccc agaaagtcac gcggggaata tcctgcctgt gttttaccag aatggagaaa
    16321 atttcggcat atttgtcggc cagcttcttt tgggtttcgg ccgccgcggc ctgaggatct
    16381 tctgtgcttt ggtggctgac ctgactgacg tctgccccgc gatagccgcg cgtctgtgta
    16441 cgaatgtcca gctcggtaat catcaccttc acgccggtag ccgccagcgt tgtgatggca
    16501 tattccagat cctccgcctt cggatatgtc aggcccccgt gcagctggat gccaacgccg
    16561 tcaatgcgaa gccctttgga ctgaagatgc ttgacaatgg cgaccgcccc atcgcatttt
    16621 gcccttgagg tttcgagatt atagtcgttg tagtacaatt cgacatccgg atcggcggca
    16681 tgagcgtatt cgaatgcctt ggcgatatgg tcatactgct ggtcgggatt gtcctgaccg
    16741 atgattttga gccattgcgt actgcggaga gttccgtttt cattgagcgc ctcattgacg
    16801 acatccca
//
LOCUS       DAOTBO010000063        16697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2868_length_16697_cov_5.377599, whole genome shotgun sequence.
ACCESSION   DAOTBO010000063 DAOTBO010000000
VERSION     DAOTBO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_16697_cov_5.377599"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1484)
                     /gene="argH"
                     /locus_tag="PLE88_08275"
     CDS             complement(93..1484)
                     /gene="argH"
                     /locus_tag="PLE88_08275"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOM76495.1"
                     /translation="MTRQEKSWEKRLSGPADPLAADFVESLSYDRRLYKYDIAGSIAH
                     AEMLAEQKIITKSEAADIKKGLLEIGEEISAGRFTFDKTQEDIHMAIESALVAKIGDA
                     GKKLHTGRSRNDQVAVDIRLWMRDEIEILQTRITRLQAAFVRLSEKYTDAVMPAYTHL
                     QRAQPATIASYLLSFVEMLARDWSRLKDCRDRLNVCPLGSGAVAGSTLPLNREMTAEN
                     LGFRDITYNSMDAVSDRDFCAEFIFCCSLIAAHLSRLAEDWIIYTSSEFSFIRTDDAY
                     CTSSSMMPQKRNPDMLELIRGKTGTIYGALMAMLTILKAQPSAYNRDLQEDKIHVFAA
                     ADTTDACLQMAAAIVSHTAFNTKRITDSLDEGFLDATALAEYVVRKGIPFRQAHGIVG
                     SLVAQCEKEGKKKLAELSIEQFQAACDKIDLDVFNTLNPKGVCQSYQTAGSAGPEAAK
                     AQIAYWKNKLKEQ"
     gene            complement(1569..2510)
                     /locus_tag="PLE88_08280"
     CDS             complement(1569..2510)
                     /locus_tag="PLE88_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76496.1"
                     /translation="MVFPKKEKKQKHKRNIVLDWLGYAAVRIILCILYLFPVRWNLQF
                     ACFLGRLMWKHYKRGRIRALENLRASFPDKEDGWLEEVGRRSFEHIVMLVIDLLFTPR
                     LVRKDNWRQYARVINIERAKWMMREGKGLLMLTGHYGNFEIMGYALSVFGFNVYSIAR
                     PLDNPFINNYLYGVRQRSGQKIIDKKGAADQMVELAEQGATLCFIADQDAGKKGIFVD
                     FFGRKASAYKSIGLLAMQYRMPIVVGVCRQIPGQFFFEAEVGRIIMPDEWQDRDNPLQ
                     WITQEYTAELEKLIRKDPAQYWWLHRRWKTRPKEERQ"
     gene            2751..3740
                     /gene="sppA"
                     /locus_tag="PLE88_08285"
     CDS             2751..3740
                     /gene="sppA"
                     /locus_tag="PLE88_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOM76497.1"
                     /translation="MVYEPAPKKKGTGWKIFWGIVITLSLLANGIMLLAMIGMGAVLV
                     GGVSSSDEGVVEKTIIDGARTKKIAVISIKGIIDDSMSEWVRRQLKTAEEDNAVKGLI
                     VLIDSPGGAVSSSDQIHYYIRRFKQNTGKPVLGFMQSVAASGGYYSAVACDQIMAEPT
                     VITGSIGVIMNHLVIKGLLEEKLGINPVTLKSGQRKDWPSMFSETTEEEKQYLTEKVI
                     TPAYERFVDLVAEGRKDVLTKDAIRRLADGSIFTAPEAYEKKLIDDIGYFDAAVEKAQ
                     QMANISNAQVVTYQEVFSLWSMLGAQSRNRINLESEILEKLAAPRLLYLWDGK"
     gene            complement(3803..4864)
                     /gene="purN"
                     /locus_tag="PLE88_08290"
     CDS             complement(3803..4864)
                     /gene="purN"
                     /locus_tag="PLE88_08290"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOM76498.1"
                     /translation="MGQQVELFTDGGSRGNPGPAAAAFILKDENGRSILARGLFLGQT
                     TNNIAEYTAVKEGLTAAQQQGARSIRLFSDSQLLVRQLNGQYKVKSSNLRSLYGDCMN
                     LLKSFDSWQVNYIPRERNTEADTLVNRAMDQKGDVNAIAQRPNPKGKTLRLGILLSGG
                     GRTMVNIQDQIKAGCLNAQIVLVISSRSDVAGNERARQIGLEPVIIRKKDYPDIADFS
                     CRIAEELDKANVDLVIQAGWLCLWQIPPQYENRVMNIHPALLPAFGGKGMWGHHVHQA
                     VLKAGCKVSGCTVHFCTNEYDAGPIIIQRVCEVKDDDDADSLAARVFEQECIAYPEAI
                     RLFSDGRLKIIDGVVKRNG"
     gene            complement(4932..5672)
                     /locus_tag="PLE88_08295"
     CDS             complement(4932..5672)
                     /locus_tag="PLE88_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOM76499.1"
                     /translation="MGPVLHGLIKLQRVENRLRAIKTKLARCRRGVLFQENQLRTLQS
                     GLEAKQEEIKLARLQVSRLELELKERDEQISKLRNQLNLARTNKEYSALLTELNTAKA
                     DDSKLETQVLEMMKNIEADEAACEEIKKQIETQRVKVDEVRKEAEAKTAELEKDIAEI
                     QAEWDAAARDIPPAALAIFSRVAENYDGEAMAEVERVDENSSSYSCGGCFMGIPAEVV
                     NVLSGKKDDIIRCSNCTRILYVKEAENQ"
     gene            5867..6985
                     /locus_tag="PLE88_08300"
     CDS             5867..6985
                     /locus_tag="PLE88_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOM76500.1"
                     /translation="MKDLLKNLITAAPTLENGEYNAAAVLLNYFQQHGIPVQIDLWDH
                     NRANVIASIGQQKPDIPALVIGAHIDVVPANSQQWTTEPFKPVEKDGKIYGRGAADML
                     GGLCAAADAMRQIFCEKKDLKGRVILAATAGEETDSCGVKRFVETVRQNISNVIGILI
                     PEPTDLKIMRAHRGILWVKIETFGKTAHGSMPHLGINAIRKMNELLNHIEKWAIPHIP
                     HPLLGGCSVSPNRIMGGSATNIIPDKCTLELDIRTLPNQEHQDIVKLIESMLDQVKAV
                     DPDFEASVSILRTCPSMETPEDNPFIQTVCRASGTAKTYAAGFTTDGPLFAQLSKSIV
                     ILGPGDSTLCHKPNEYIGINELKKAKELYQKIICNLFI"
     gene            7153..8271
                     /locus_tag="PLE88_08305"
     CDS             7153..8271
                     /locus_tag="PLE88_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76501.1"
                     /translation="MKSSVKKCAFIYFCLSLAQAAFCAAVILNEYNAVAPDTFLGGGT
                     FAADAKGGRASDYYFGRIMGNGGDWFELIVITDHLDMRNWKLDIYENDVLDETLDLTN
                     HSIWSDLRSGTIITVSEDVPSDISYNPADGDWWINVQANNTADGRYIEASSFPVSSSN
                     WQLRIRNAAGAIIFGPAGEGISPAAGIGSDEVFKLKANPSSTTAANSADYDGDDKLST
                     FGQPNQWGQQNLNSLRTVAPAPSTLTLIAPNGTEVIKSGSFYPIEWQSTGTINNVLIE
                     FSTDNGQSWSGVYPPNIGNSGRYVWQVPVLNTDNGLIRITNMGNPAVMDASNAAFSIY
                     ECPLKGDATDDCIIDLNDLAVIANSWLDCANPYLNCMP"
     gene            complement(8353..10965)
                     /gene="glgP"
                     /locus_tag="PLE88_08310"
     CDS             complement(8353..10965)
                     /gene="glgP"
                     /locus_tag="PLE88_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOM76502.1"
                     /translation="MQTVHTFTVKPSLPEQLKDLQVIVRNLYWTWHYDITEIFRRIDY
                     DLWKQCIHNPVKMLGMVSQARLEDLARNEGFLYQLKQARETLEKTLNAPSWFNKIYAR
                     EGHKTLIAYFCAEFGIHESMPIYSGGLGMLSGDHLKSASDLGIPLVGVGLMYQKGYFR
                     QYLNTDGWQQEHYIDNDFHNMPLELVQKENGHPVLIGIQFPDRTVQAQIWKAMIGRTP
                     LYLLDTNLTVNSFHDRTITQTLYGGDSEMRICQEIVLGIGGLKALFALGLEPTVCHMN
                     EGHAAFMALERVRKLRSRYNMTFEEALEAARASNVFTVHTPVAAGNDEFPVEMIDKYF
                     SHYYGTLGINREQFLALGRVHPNDSHESFKMPVLALRTSTYRNGVSQLHGEVSRKIWS
                     GLWPELPVSEIPITSITNGIHAKTWLSPELNSLYERYLGSRWADEAVDKSIWHNLDQV
                     PDEELWRIHQRGKERLVAFARTRLKRQLQRRGAFHTELSWAEEVLDPEALTIGFARRF
                     ATYKRGNLLLREPQRLIKLLSNTETPVQFIFAGKAHPRDTAGKEIIRQLVHFASENPE
                     IRRRLIFLEDYDMDIARYLVQGVDVWLNNPRRPMEASGTSGMKAAINGVLNMSTLDGW
                     WCEGYIPDGGWIIGAGEEYDDLSYQDQVESQAIYNLLEQEVVPLFFNRGKDRLPRRWI
                     RRMKNTIKWCAPRFNTSRMVAEYTRRFYHPADLRWQELTANEMERVKALSLWKQKIRQ
                     SWSDLQIRSVETKTVDGEPVGDYGGKQPELSVGSQVQVQSRVHLGRLEPTDISVQIYY
                     GRVDSSGQIKDGQISEMAYVDNSLGPERVGQFSGTIPCRISGQRGFALRILPRHPDLV
                     EPYESGLVLWETGR"
     gene            complement(11283..12134)
                     /locus_tag="PLE88_08315"
     CDS             complement(11283..12134)
                     /locus_tag="PLE88_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOM76503.1"
                     /translation="MDVAMIDDQMLPMCDLAKPYLDRGLYFGDGVYEVIRSYDGQIFA
                     LDEHLNRFERSLREIQINTVNLSVIKQKIVAAVERSGYSNAKIYFHITRGSEPRDHLP
                     SDGLLPNFFMTVSQLHDSPEVKEKGIAVAAYPDLRWKRCDIKSLNLLPNVLARIEVKK
                     QGASEAILVGDDGTITEGAGSAFFAVNGKEKVIITRPLGHEILPSITRMMILPVAQNA
                     GLAVVEKTLTPQQAAQCDELFIAVTTKDIVPVAYFNGKPISGGRCGQWTGRLIREFRE
                     FVHQRRQ"
     gene            12525..12851
                     /gene="rplU"
                     /locus_tag="PLE88_08320"
     CDS             12525..12851
                     /gene="rplU"
                     /locus_tag="PLE88_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOM76504.1"
                     /translation="MYAVIEQGGKQYKVSQGDVITIELTEVPADAKTIELGNVLFVSD
                     GQKVQIGTPYLVGAKVIATVKVSGQSPIVTGPKLYPTLFRRRCLKKRTGHRQKYLQVI
                     IEGIEA"
     gene            complement(12946..13329)
                     /locus_tag="PLE88_08325"
     CDS             complement(12946..13329)
                     /locus_tag="PLE88_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM76505.1"
                     /translation="MAKKVLVIDDDSDFVESIVNLLEARGYNVASASNGKEGVAAAKA
                     EKPDVILLDVMMTTKDEGFNVARELQSIEGIKGTPVIMVTGVRKEMNLPFGFEPDETW
                     LPVKQILEKPVKPETLLAAVADALK"
     gene            complement(13464..13955)
                     /gene="nuoE"
                     /locus_tag="PLE88_08330"
     CDS             complement(13464..13955)
                     /gene="nuoE"
                     /locus_tag="PLE88_08330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOM76506.1"
                     /translation="MTEMTQEKMDELLGFIDSVSTKPYSDSYLIAVLHKVQSLYGYLP
                     REAMDIVAQKMQIPTAHIWGVATFYHYFNLNPVGKHTISVCMGTACYVKGAEKVLNTL
                     KTELKISVGQTTEDKLFTLQEARCLGACGIAPVMMVDDKIYGELDAKKTIDIIHQYRK
                     AAQ"
     gene            complement(13959..15740)
                     /locus_tag="PLE88_08335"
     CDS             complement(13959..15740)
                     /locus_tag="PLE88_08335"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOM76507.1"
                     /translation="MANTITIYINHKKYQVEPGQTIMQAADKLGFHIPRLCYHPKLSI
                     EGACRVCIVEVEGARNYVASCAFPVSDGMRIHTNTQALRRARRDIIELILDNHPQDCH
                     VCERDGNCELQRLASAMGIRARHFEGQRKRYEKDLSSEAVIRDPEKCILCGRCVRMCS
                     EIQQVHNLTQAHRGFNTVVMPAYNMPMGESVCTACGQCINVCPTAAFLEKNYTQELFD
                     KLNDPHLVKVTQFAPSVRAAVGEAFGLEPGRNMEGQLIAALRKLGFDYVFDTQFSADL
                     TIMEEASEFLERLQNGGVLPMITSCSSAWMKCLEQFYPDLIQNVSTCKSPMSMQGAVT
                     KTYWAQKMGIDPKKILSTAVMCCTAKKYEAARPELMVDGMRGVDIVVTTREIAWMIKS
                     AGIDLLHIKPEEPDHPLGMSSGAGAIFGVTGGVMEAAVRTAYELYTGQTLVDIEIANV
                     RGLKGIKEGTLEMDGKEIRVAVAHGLGNACRLLEQVRKDPSRYHFIEIMGCPGGCIGG
                     GGQPYAQINTIPLDDEALQKRAQVLYDLDRAKTIRRSHDNPDVQKLYKEFLKSPLSPV
                     SHKYLHTHYKAKTPVGIIPKKERESGV"
     gene            complement(15749..>16697)
                     /locus_tag="PLE88_08340"
     CDS             complement(15749..>16697)
                     /locus_tag="PLE88_08340"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOM76508.1"
                     /translation="FVCGEETALIHSIEGVRGMPRPRPPYPSVSGLFGKPTLINNVET
                     WANIPVIILDGGDWFSSIGTKTSKGTKVFALAGKVLNTGLVEVPMGTTLRQIVYDIGG
                     GIPNNKKLKAIQTGGPSGGCLPADYLDTEIDYESLAKAGSIMGSGGMIVIDEDTCMVS
                     LAKFFLEFTQDESCGKCTPCREGTKRMLEILTRITEGKGRTGDIEKLERLGQMIKSAS
                     LCGLGQSAPNPVLSTIKNFREEYEEHIEHKKCRAGQCSALLSYQINEKCVGCGACKRV
                     CPVGAIEGIRKEKHRILPDKCIRCGQCYQACKFEAITKA"
BASE COUNT         4309 a         4200 c         3737 g         4451 t
ORIGIN      
        1 atcggctttg gattgttcag caaaccaagc tgttatgata tcttccttat tgctcatcaa
       61 ttcgttggga ttggcggttt tcaaaccctc ttctactgtt ccttcagctt gtttttccaa
      121 tatgcaattt gcgctttagc cgcttcggga ccggcagaac cggcagtttg gtaggattgg
      181 caaacgcctt tcggattcag tgtattgaaa acatccaaat caattttgtc gcaggcagct
      241 tggaattgct cgatggacaa ctccgccagc ttctttttgc cctctttttc gcattgggca
      301 accaaactgc cgacaatgcc atgtgcctgc cggaaaggaa tccctttgcg taccacatat
      361 tcggccagag ccgttgcatc cagaaaaccc tcatccaagc tgtcggttat gcgcttggta
      421 ttgaacgctg tatgactgac aatagcggcg gccatctgca ggcaggcatc tgtggtgtct
      481 gcagcagcaa aaacatgaat cttgtcttcc tgcaaatccc ggttatacgc agacggctgg
      541 gctttaagga ttgtcagcat cgccatcagc gctccataaa tcgttcccgt tttgccgcga
      601 atcagctcca gcatatccgg attgcgcttt tgcggcatca tactcgaact ggtgcagtag
      661 gcgtcatctg tgcgaataaa agagaactct gatgaggtat aaataatcca gtcttcggcc
      721 aagcgggaca agtgcgccgc aatcagcgaa cagcagaaaa taaattctgc acagaaatct
      781 ctgtcggata cggcatccat gctgttgtat gtgatgtccc tgaagccgag attttctgcc
      841 gtcatttcac gattcagcgg cagggttgaa ccggctaccg ccccgctgcc taagggacag
      901 acattcagcc ggtcgcggca gtccttcagc cggctccagt cgcgtgcaag catttcaaca
      961 aaactcagca gataggaggc aatcgttgcc ggctgggctc tctgcagatg cgtataggcg
     1021 ggcatcaccg cgtctgtata tttttccgac aggcgcacaa aagcagcctg cagccgggta
     1081 atgcgtgtct gcaggatttc aatctcatcc cgcatccaaa ggcggatatc aacagcaacc
     1141 tgatcattgc ggctgcggcc ggtatgcagt tttttgcctg catcgccgat tttagccacc
     1201 agcgccgatt caatggccat atggatatct tcttgggttt tatcgaaggt aaatcgaccg
     1261 gcgcttattt cctcaccgat ctccaataat ccctttttga tatctgcggc ttccgatttg
     1321 gtgataattt tctgttcggc cagcatttca gcatgggcaa ttgaaccggc aatatcatat
     1381 ttgtagagcc gccgatcgta tgaaagggat tccacaaaat cggctgccaa cggatccgcc
     1441 gggccgctaa gacgtttttc ccagcttttt tcctgtctgg tcatatttgt actccatgcg
     1501 agggtttata gtgcaaacgc catgatatag ttcaatcggc aacgtgtcaa gaaatgcttt
     1561 ggatgcaatc attgccgctc ttctttgggg cgggttttcc accgccggtg aagccaccag
     1621 tactgggcag gatctttgcg aatcaacttt tcaagttcgg cagtatattc ctgcgtaatc
     1681 cactggagag gattgtctct gtcctgccat tcgtcgggca taataatccg tcctacttcg
     1741 gcttcaaaaa agaattggcc gggtatctgc cggcacaccc ctactacaat cggcatacga
     1801 tactgcatgg ccagcaatcc gatgcttttg taggcgctgg ccttgcggcc gaaaaaatcg
     1861 acaaagattc cctttttgcc tgcatcctgg tcagcaataa aacacagcgt tgctccctgc
     1921 tcggccagtt caaccatctg gtcggcggca ccttttttat cgataatttt ttggcctgac
     1981 ctctggcgca cgccgtaaag ataattgttg ataaatggat tatccagcgg gcgggcaatg
     2041 ctgtaaacat taaagccgaa gacactgagg gcatacccca taatctcaaa gttgccgtaa
     2101 tggcctgtca gcatcaaaag ccccttgcct tcccgcatca tccatttggc acgctcaata
     2161 ttaatgacac gggcatattg acgccaattg tcttttcgta caagccgggg ggtaaacagc
     2221 aaatctataa ccagcatcac aatatgctca aatgagcggc ggcccacttc ttccagccat
     2281 ccgtcctcct tatcaggaaa actggcccgc agattttcca acgcccgaat ccggccgcgc
     2341 ttgtaatgct tccacatcag ccggccgaga aagcaggcaa actgtaaatt ccatcgtacc
     2401 ggaaacaaat ataggataca aagaataatc cgtacagccg catatcccag ccaatccagt
     2461 acaatatttc gtttgtgttt ttgttttttt tctttcttag ggaaaaccat ttttgcgata
     2521 tagtatattg attatattgt gattcgtatg ccctattatg gtgttataat gtcgcctgtc
     2581 aagctttgat tgacagccca aacatatctg cgtataatta ggaaacatat aattgataaa
     2641 tacgaaaggc aaaaaagatg gaatctaact ccaattcgtc ccagcaatcg ccaacagagc
     2701 agctgtcttc gagtctgcct ccggcctgca atattccgga ctttagcaat atggtttatg
     2761 aaccggctcc caagaaaaaa gggaccggct ggaaaatctt ctggggcatc gttattaccc
     2821 tatcgcttct ggccaacggg attatgctcc tagccatgat tggtatgggg gccgttcttg
     2881 tgggaggtgt ttcaagcagc gacgaaggag tcgttgaaaa aacaataatc gacggagccc
     2941 gcaccaaaaa aattgctgta atcagcatca agggaattat tgacgattca atgagcgagt
     3001 gggtcagacg gcagcttaaa accgccgaag aagataatgc tgttaaaggg ctgattgtcc
     3061 tgattgacag ccctggcggg gccgtatcat ccagcgatca gattcattat tacatccgcc
     3121 gctttaaaca gaacaccgga aagccggtgc ttggttttat gcagtctgtg gcggcatcgg
     3181 gcggttatta ttcagccgtc gcctgcgatc agattatggc tgaacctacg gttattaccg
     3241 gctcaatcgg ggtaattatg aaccatttgg tcatcaaagg actgctggaa gagaaacttg
     3301 gcatcaatcc tgtaaccctc aaaagcggtc agcgaaaaga ctggcccagt atgtttagcg
     3361 aaaccaccga agaggaaaaa caatatctga ccgaaaaagt cataacaccc gcttatgagc
     3421 gatttgtaga cttggtcgcc gaagggcgaa aggatgtgct caccaaagat gccatccggc
     3481 ggttagccga cggcagtatt tttacagcac cggaagccta tgaaaagaag ctgatcgacg
     3541 atatcggcta ttttgatgct gctgttgaaa aggcccagca gatggcaaat atttccaatg
     3601 cacaggttgt aacctatcag gaagtctttt cgctctggtc aatgctcggg gcacaaagca
     3661 gaaaccggat taaccttgaa tcagaaattc ttgaaaaact ggctgctccg cggctgctct
     3721 atctctggga cggcaaataa aaaagcctaa taaaagcttc agagagaatc gtcctgcaaa
     3781 tagtcagacg gcggatgcaa actcagccgt ttctttttac cactccatcg ataattttga
     3841 gccgcccatc ggagaataat cggatcgctt cggggtatgc gatgcattcc tgttcaaaga
     3901 cgcgtgctgc taaagaatct gcgtcatcgt cgtctttgac ctcacagacc cgctgaatga
     3961 taatcgggcc ggcatcatac tcattggtac agaaatgcac ggtgcagccg gacactttgc
     4021 agccagcctt caagactgct tgatgcacat gatggcccca cattcctttg ccgccaaagg
     4081 ccggcagaag cgccgggtgg atattcatca cgcggttttc atactgcggg ggtatttgcc
     4141 acaggcacag ccagccggcc tgaatgacca aatccacatt tgctttatcg agttcttcgg
     4201 caatacgaca actgaaatct gcgatatccg gataatcttt ttttcggatg atcaccggtt
     4261 ccagaccgat ttgcctcgcc cgttcattgc cagcaacatc agatcgagaa ctgattacca
     4321 aaacgatttg agcattcaga cagccggctt tgatttgatc ctgaatgttg accattgtgc
     4381 gtcccccccc gctcagcagg atgcccaaac gcagcgtttt gcctttcgga ttaggccttt
     4441 gcgctatggc attcacatcg cctttttggt ccattgcccg attgacaaga gtatcagctt
     4501 cggtatttct ctctcgcgga atataattaa cctgccatga atcaaaagac ttcagcaaat
     4561 tcatacaatc gccataaaga gaacgcagat tgctgctttt gaccttatac tggccgttca
     4621 actgccgcac cagcagctgg ctgtcgctaa acaggcgtat cgatcgagcc ccctgctgtt
     4681 gtgcggctgt caaaccttcc ttgacagcgg tatattctgc aatattattg gtggtttgcc
     4741 cgagaaacag accgcgggcc aaaatggagc gtccgttttc atctttcaaa ataaaagctg
     4801 cagccgccgg tccgggattg ccgcggctgc ctccatctgt aaaaagttcg acttgctgac
     4861 ccacgtttat tctcttggaa tgctgccttg ccagaaaaca gcacgaggca ttaaaaaata
     4921 ccgtctaagg cttattgatt ttcggcttct ttgacgtata aaatccgcgt gcaattgctg
     4981 cagcggataa tatcgtcttt cttgccgctt aaaacattca ctacttcagc aggaattccc
     5041 ataaaacagc cgccgcagct gtaagaggaa gagttctcat cgactcgttc cacctctgcc
     5101 attgcttctc catcataatt ttcggctacc cggctgaata ttgccaatgc tgccggcgga
     5161 atatcacggg cagcggcatc ccactcggcc tgaatttcag caatatcctt ttcaagttcg
     5221 gcagttttgg cttccgcttc cttgcgaacc tcatctactt taacacgttg ggtctctatt
     5281 tgctttttga tttcctcgca ggcagcttca tctgcttcga tattcttcat catctccaag
     5341 acttgtgttt caagcttgga atcgtctgct ttggcagtat tcagctctgt cagcagcgca
     5401 gaatactctt tgttagtgcg tgctaaattt aactgattgc gaagtttgct gatttgctca
     5461 tcccgctcct tcaattccag ttccagacgg ctgacttgga gtctggccag tttgatttcc
     5521 tcctgttttg cttcaagccc gctttggagt gtccgcagct gattttcttg aaacaatacg
     5581 cctcttcggc atctggccag ttttgttttg atcgcccgaa gacgattttc cacacgctgc
     5641 agtttaatta acccatgtaa aaccggtccc attcgacatc cttttttaac ttgtaaatat
     5701 ccccacccat aattccaagt gttgtattat gatataatta aaagcttctg caacagattt
     5761 ttttgccttt ttccaatttg catcgcaatt caaaacaaaa aactgtataa ttttatacct
     5821 ttattaaaaa agcggaaacc gtaatataag atatttcagg gcttttatga aagatttgtt
     5881 aaaaaacttg attacagcag ctccaaccct cgaaaacgga gaatataatg ctgccgccgt
     5941 acttttaaat tacttccaac aacatggtat ccctgttcaa atcgatttat gggatcataa
     6001 ccgtgccaat gtcatcgcat ccatagggca gcaaaaacct gacattccag ctcttgtaat
     6061 tggagcccat attgatgtgg tgccagcaaa cagccaacag tggaccacag aaccattcaa
     6121 accagtagaa aaagatggaa aaatatatgg ccgcggcgct gcggatatgc tggggggact
     6181 ttgtgctgca gcagatgcta tgcgtcaaat attttgtgaa aaaaaagatt tgaaaggccg
     6241 tgttattctg gccgccactg ccggcgaaga aactgacagc tgcggagtca aacgattcgt
     6301 tgaaacagtc cgtcagaaca tcagcaatgt aataggcata ttaattccag agccgacgga
     6361 tcttaaaata atgcgtgcac atcggggcat tctttgggtc aaaatcgaga catttggcaa
     6421 aacggctcac ggctcaatgc cgcatcttgg tatcaatgct ataagaaaaa tgaatgaatt
     6481 actcaatcat attgaaaagt gggctattcc gcatattcca catccactat tgggcggttg
     6541 ttctgtcagt cctaatcgta taatgggcgg cagtgccact aatataatac cagacaaatg
     6601 cactcttgaa cttgatattc gtacattgcc caatcaagag catcaggata tagtgaagct
     6661 aattgaatca atgctggacc aagtgaaagc tgttgatcct gattttgagg cgtctgtctc
     6721 tattctgcga acctgtccat ctatggaaac acctgaagat aatccattca tccagactgt
     6781 ttgcagagct tcgggcacag ccaaaacata tgcggccggc tttacaactg atggtcctct
     6841 atttgcgcaa cttagtaagt ctattgtaat tttgggcccg ggcgatagta cactgtgcca
     6901 taaacccaat gaatatatag gaataaatga actcaaaaaa gccaaagaat tatatcaaaa
     6961 gattatatgc aatctcttta tctaaaacaa aacagaaaaa tagtatgtct tctttctctg
     7021 aatgacaaca acaagatgga aaaatcttga caatttcggg cgttttttat agtatatata
     7081 tttagtacat tcaggattat cctgaaatgt tcattcatct ttgtggggac tttgttttcg
     7141 gagaggtgac agatgaaatc ttcagttaag aaatgtgcct tcatttactt ttgcttgagt
     7201 ttagcacaag ctgctttctg cgctgctgtt attcttaatg aatacaatgc tgttgctccc
     7261 gatacgttct tgggcggcgg gacatttgcc gcagacgcaa agggaggaag ggcatcggac
     7321 tactactttg gccggattat gggtaatggc ggcgactggt ttgaattgat tgttattact
     7381 gaccatctgg atatgcgcaa ctggaaactg gatatttatg agaacgacgt tctcgatgaa
     7441 acgcttgacc tgaccaatca cagcatttgg tctgatttaa gaagcggaac gattattaca
     7501 gtttctgaag atgttcccag cgatatcagc tacaaccccg ctgacggcga ctggtggatt
     7561 aacgttcagg ccaataacac cgccgacggc cgttatatcg aggcatccag ctttcctgtc
     7621 agcagctcca attggcagct tcgcattcga aatgcagctg gggcaataat ttttggacct
     7681 gccggtgaag gcatcagccc tgctgctgga atcggcagcg acgaagtttt caagctgaaa
     7741 gcaaatccat cctcgacaac tgctgccaac tctgccgatt atgatggtga tgacaaattg
     7801 agcacattcg gccaacccaa tcaatggggc cagcagaatc tgaattcgct tcggacagtt
     7861 gctccggcac cgtctacatt gacactgatt gctcccaatg gaacagaagt catcaaaagc
     7921 ggcagtttct atcctataga atggcagtca acaggcacaa ttaataatgt tttaatcgaa
     7981 ttttcgacag ataacggaca gagctggtct ggggtatacc ctcctaatat cggcaacagc
     8041 ggccgctatg tctggcaggt acccgtgctc aatacagata atggtttgat acggataacc
     8101 aacatgggca atccggccgt tatggatgcc agcaatgcag ccttctcgat ttacgaatgc
     8161 ccgctgaaag gtgatgcaac ggatgattgt attatcgacc tgaacgattt agcagtcatt
     8221 gccaattcgt ggctggattg tgccaatccc tatttgaact gtatgccgta gagatctgcc
     8281 gactttactc aggaaaacgc atatgtcaaa aaagccgtat ttgaaaatac ggcttttacg
     8341 cttaaaggtt tgtcatcggc cagtttccca gaggaccaaa ccggactcat acggttccac
     8401 caaatccgga tgccgcggca gaattcgcag agcaaagcca cgctgtccgg aaattcggca
     8461 gggaatagtc ccgctgaact gccccacgcg ttcgggaccc agagaattat caacataagc
     8521 catttcagaa atctgaccat ctttgatttg tccggaggaa tctaccctgc cgtaatatat
     8581 ctgcacagat atatctgtcg gttcgagccg gcccagatgg acacgcgact gtacctgcac
     8641 ctgcgatccg actgacagtt cgggctgttt gccaccgtaa tcgccgaccg gttcaccgtc
     8701 aacagttttg gtttcaaccg accgtatctg caaatctgac caagactggc gtatcttctg
     8761 cttccacaga gacagagcct tgacgcgttc catttcattg gccgtcaatt cctgccagcg
     8821 caggtccgca gggtgataaa agcggcgtgt gtattccgca accattcggc ttgtattgaa
     8881 tcgcggggca caccatttga tggtattctt catccggcgt atccagcggc gcggcaggcg
     8941 gtctttgccg cgattgaaaa acagcggtac aacttcctgt tcaagcaaat tgtaaattgc
     9001 ctgagactct acctgatctt gataggataa atcatcatat tcctctccgg cgccgataat
     9061 ccagccgccg tctggtatat atccttcaca ccaccagccg tccagcgtac tcatattcag
     9121 tacgccatta atagccgcct tcatcccgct ggtaccgctg gcttccatcg gacgccgcgg
     9181 gttgttaagc caaacatcga cgccctgcac aagataacgg gcaatatcca tatcgtaatc
     9241 ttcaagaaat atcagccggc ggcgaatttc cggattttcc gaagcaaaat gcaccagctg
     9301 gcgaattatt tctttaccag ccgtatcgcg gggatgggct ttgccggcaa aaataaactg
     9361 caccggcgtt tcggtattgg acagcagctt gataagacgc tgcggctccc gcaagagcag
     9421 attgccgcgt ttataggtag caaaccgccg ggcaaatccg atcgtcagag cttccggatc
     9481 cagaacttct tcggcccagc tcaattcggt atgaaatgct cctcgacgct gcagctgacg
     9541 tttaagccgg gttcgtgcaa atgccacaag ccgttctttg ccgcgctggt gaatacgcca
     9601 taattcttca tccggcacct gatccagatt atgccagatg gatttatcga ccgcctcgtc
     9661 cgcccagcgg gaacccaaat aacgttcgta aagcgaattc agctcgggac tcagccatgt
     9721 tttcgcatga atgccgttgg tgattgaggt aatggggatt tccgacaccg gcagttcagg
     9781 ccataaaccc gaccagattt tccgcgatac ctcgccatgg agctgactga caccgtttcg
     9841 gtaggtgctg gttcgcaaag ccaataccgg cattttgaaa ctttcatggc tgtcattggg
     9901 atgcacgcgt cccagtgcca gaaactgttc acgattaata cccagtgttc cataataatg
     9961 gctgaaatac ttatcaatca tctccacagg aaattcatca tttccagccg ccacaggcgt
    10021 atgaacagtg aacacattgc tggcacgggc ggcttcaaga gcttcttcaa acgtcatatt
    10081 gtagcggctt cggagcttgc gcacccgctc cagcgccata aatgctgcat ggccttcgtt
    10141 catatgacag accgtcggct ccaatcccag cgcaaaaagg gcttttaagc ctccaatccc
    10201 cagcacaatt tcctgacaga tgcgcatttc agaatcaccg ccgtacagcg tctgggtgat
    10261 cgtacggtca tggaatgaat tgaccgtcag atttgtatcc agcagataca aaggcgtacg
    10321 cccaatcatt gccttccata tctgggcctg caccgtgcgg tcggggaact ggatgccaat
    10381 cagaacagga tggccgtttt ccttctgcac cagttccagc ggcatattgt ggaaatcgtt
    10441 gtcaatataa tgctcctgct gccagccgtc ggtgttaaga tattgacgaa aatatccctt
    10501 ttggtacatc agaccgactc ccacaagcgg aatcccaagg tcggaagcac tcttgagatg
    10561 atcccctgac agcataccta aaccaccaga ataaataggc atggactcgt ggataccaaa
    10621 ttcagcgcaa aaataagcaa tcagagtctt gtgcccttct ctggcataaa tcttgttaaa
    10681 ccatgatgga gcattaagtg ttttttccag tgtttcacgg gcctgtttaa gttgataaag
    10741 aaagccctca tttcgggcca agtcttccag acgggcctgt gaaaccatgc ccagcatctt
    10801 gacggggtta tgaatacact gcttccacaa atcataatca atacggcgga agatctccgt
    10861 gatatcataa tgccaggtcc agtaaagatt gcgaacaata acctgcaaat ctttcaactg
    10921 ttccggcagt gaaggcttaa ctgtaaatgt atgtaccgtc tgcatttgtt gttaatcctt
    10981 agtgctgtta tttagattct taaactttac aagcaactgc ttactgttta tcgtcatctt
    11041 ttgttcggct taaaaacgga tttgcttaat ggaaaaacac tgaaattcca gtgcagctaa
    11101 cgaaaaatca ttcatagaat tcgacttttt aggcctttaa caaaagttaa ataggtccta
    11161 taacacatat taaatgagca tttatatcct gatgtaaagg aatacaaaat tcatacgttc
    11221 aatataaatt attgtagtcc ttacagggct gcgatatcta agatagggtt tatcttgtgt
    11281 ctttattgtc ttctttggtg cacaaactct cggaattcac gaatcagtcg tccagtccac
    11341 tgaccgcagc gtcccccgct gatgggtttc ccattaaagt atgcaacagg cacgatatct
    11401 ttggttgtaa cggcgataaa taattcatcg cactgagcgg cctgttgagg ggtcaaagtc
    11461 ttttcgacaa ctgccaaccc cgcattctgg gccaccggca aaatcatcat ccgcgttatc
    11521 gacggcagaa tttcatgccc caacggacgg gtaataatta ccttttcttt gccattcaca
    11581 gcaaaaaacg cggatccggc accctcggtg attgtaccgt catcgccaac cagaattgct
    11641 tcactggccc cctgcttttt aacttcaatg cgtgccaaca cattgggcag aagattaagc
    11701 gacttgatat cgcaccgctt ccaccgcaaa tctggatatg cagcaactgc aattcctttt
    11761 tcttttacct cgggactatc gtgaagctgg ctgacagtca taaaaaaatt aggaagcagg
    11821 ccgtcactgg gaagatgatc acgaggctcc gaaccccggg taatatggaa ataaattttt
    11881 gcattgctat agccagaccg ttccaccgcc gccactatct tttgcttgat cacactgaga
    11941 ttcaccgtgt ttatctgaat ctcacgaaga ctccgctcaa atcgattcag atgttcatct
    12001 aaagcaaata tctgaccgtc ataagaacga ataacttcat aaacgccgtc accaaagtat
    12061 agccccctat cgagataggg tttagccaga tcgcacatcg gcaacatctg gtcatcaatc
    12121 attgcaacat ccatattgcc atttcctttt ttaaaaaagt agttttcaac aatcatatcc
    12181 gcaataatgc tgccttgcaa ggccaaacga taatgccggc tctggattgg attgtgccca
    12241 ttagtgcgat aagtattgga agggacaaat acagatataa tctatcatta tatgcagtat
    12301 tttatttatt caatcgtgcg ttggaattta tattcataga gatatagatt acggatgcat
    12361 tgtccttaca aaaatcccta agttatttat aaaaaataag ttaagaaatt ttacctaaat
    12421 tgaaaatttt tctttacaaa tataaggcaa attcgtagaa taccgcattc ttttgattga
    12481 tgtgtgaata tttctgtaat agattgactc aatgggagtt taaaatgtac gcagtgatcg
    12541 aacaaggcgg caaacaatat aaggtatcac agggtgatgt gatcactatc gaattgaccg
    12601 aggttccggc cgatgccaaa accatcgaac tgggcaatgt actgtttgta tcagacggcc
    12661 aaaaagtgca aattggcaca ccgtatctgg ttggtgccaa ggtcatcgcc accgttaagg
    12721 tatctggcca aagtccaatc gtaacaggcc cgaagttgta tccaaccctg tttcgccgga
    12781 gatgcttgaa aaaacgaacc ggtcatcggc agaaatacct tcaggtcatc attgagggta
    12841 tcgaagccta atggaggcat ccaaaaaata aaaaccccgg caaaatcaaa gttagccggg
    12901 gtttttttgt taattcaaac aatttgctct aaattttaga tgcccttatt tgagtgcatc
    12961 cgccacggca gccagcaagg tttcgggctt gacgggcttc tcgagaatct gcttgaccgg
    13021 cagccaagtt tcatccggct caaaaccgaa aggcaggttc atttctttgc gtactcccgt
    13081 caccataata accggtgtac ccttaatacc ttcaattgac tgaagctccc gggctacatt
    13141 gaaaccttca tctttggtgg tcatcatcac atccaggaga ataacatcgg gtttctcggc
    13201 tttggccgct gcaacgcctt ctttgccatt ggaggcggaa gccacattat aaccccttgc
    13261 ctccaaaaga tttactatcg attcaacaaa atcgctgtca tcgtcaatta ccagcacctt
    13321 tttagccatt tttaactctc ctaaccttta caaagtttgt tttctgcgtg actgggattt
    13381 ataccacaaa aaacgcccca tgtcttattt tttttcctta aaagtggatt tttctcattg
    13441 acttgttatc tccaatacag tcgctactgt gcggcctttc gatactggtg aataatatca
    13501 atcgtctttt ttgcatccag ttcgccgtag attttgtcat ccaccatcat gacaggggca
    13561 attccgcagg cccccaaaca gcgggcttcc tgaagagtaa acagcttgtc ttccgtcgtt
    13621 tggccgaccg aaatcttcaa ttctgtcttt aatgtgttaa gcaccttttc ggcacccttc
    13681 acatagcagg cggttcccat acagacgctg atggtgtgtt ttccgacagg attcaaatta
    13741 aagtaatgat aaaatgttgc tacaccccag atgtgtgctg tcggaatctg catcttttgt
    13801 gcaacaatat ccatcgcttc tctgggcagg tagccgtaaa gcgactgaac cttatgcagc
    13861 acagcaatca ggtatgaatc cgaatacggt tttgtgctga cactgtcgat aaaaccgagc
    13921 agttcgtcca ttttctcttg ggtcatctct gtcatcgctt atactccgct ttcacgttct
    13981 ttcttaggaa taatccccac aggggttttc gctttgtaat gggtatgaag atacttatgc
    14041 gacaccggac ttaaggggct cttcagaaat tctttataca gcttctgtac atccggattg
    14101 tcatgacttc gccgtatggt cttagcacgg tcaagatcat agagcacttg agcccgtttt
    14161 tggagggctt cgtcatccaa aggaatcgta ttaatctgag catagggctg gccgccgccg
    14221 ccgatgcagc cgccgggaca acccattatc tcgataaaat gataacggct cggatctttg
    14281 cgaacctgct ccagcagcct gcaggcattg ccgagcccgt gagcaacagc aactcgaatt
    14341 tctttgccgt ccatctcgag cgtcccttct ttgatgccct ttaatccgcg aacattggct
    14401 atctcgatat ccaccagcgt ctggcctgta tagagttcat aggcggtacg aacagccgct
    14461 tccataacac cgccggttac accgaagata gcaccggcac cgctggacat tcctagcggg
    14521 tgatccggct cttccggctt aatgtggagc aggtcgatgc cggcagattt aatcatccat
    14581 gcgatctcgc gtgtggttac aacaatatcc actccccgca tgccatccac catcagctcg
    14641 ggccgagctg cttcatattt cttggcagtg cagcacatca cggccgtgct gagtatcttt
    14701 ttgggatcga tgcccatctt ttgagcccaa taggtcttgg taacagcccc ctgcatagac
    14761 atcggagatt tgcaggttga aacattctga atgaggtcag gataaaattg ttccagacat
    14821 ttcatccaag cactggaaca agacgtaatc atcggcagca cgccgccgtt ttggagccgc
    14881 tccagaaatt cgctggcctc ttccatgatt gtcaggtctg ccgaaaactg ggtatcaaag
    14941 acatagtcaa atccgagctt gcgcagagcc gcaatcagct ggccttccat attgcgtccg
    15001 ggctccagcc caaacgcctc gccgacagcc gcccgaacag acggggcaaa ctgggtgacc
    15061 ttgaccagat gggggtcatt gagcttgtcg aataactcct gagtatagtt cttttcaaga
    15121 aacgccgcag tcggacaaac gttgatgcac tgtccgcagg ccgtacacac actttcaccc
    15181 atcggcatat tataggccgg cataaccacc gtattgaagc cgcgatgggc ttgggtcagg
    15241 ttgtgaacct gctgtatttc cgagcacatc ctcacacagc gtccgcagag aatgcacttt
    15301 tcggggtcgc ggataaccgc ttcactggac aaatcctttt cgtagcgctt gcgttggcct
    15361 tcaaaatgac gggctcgaat gcccatcgcg gatgccagcc gctgcagttc gcagttgccg
    15421 tcgcgttcac agacatggca gtcttgagga tgattgtcaa gaatcagctc aataatatcg
    15481 cggcgggctc tgcgcagagc ctgcgtgttg gtatgaattc tcatcccatc gctgactgga
    15541 aaggcacagg atgcaacata gttgcgtgcg ccttccactt ccacaatgca gactctgcag
    15601 gctccctcaa tagacaactt agggtgatag cacagccggg ggatatgaaa cccaagcttg
    15661 tcggccgcct gcataatggt ctggccaggt tctacttgat attttttatg attaatgtag
    15721 atggtaattg tatttgccat agaaatcctc atgccttagt gattgcttca aacttacatg
    15781 cctggtagca ctggccgcac cgaatgcact tgtctggaag aatcctgtgt ttttccttcc
    15841 ggatgccttc gattgcccca accgggcata cccgcttgca agcgccgcag ccgacacatt
    15901 tttcattaat ctggtaactg agcagtgctg aacactggcc ggcacggcat tttttatgtt
    15961 caatatgttc ttcgtattcc tcacggaaat tcttgatcgt actgagtacc ggattgggtg
    16021 ctgactggcc gagaccgcac agcgaagcgc tttttatcat ctgccccagc cgttccagtt
    16081 tttcaatatc gccggttctg cccttgcctt cggtaatgcg agtcagaatt tcgagcatgc
    16141 gtttggtacc ttcgcggcaa ggtgtgcatt tgccgcagga ttcgtcctgt gtaaattcaa
    16201 ggaaaaattt agccagcgac accatgcagg tatcttcgtc gattacaatc attccgccgg
    16261 agcccataat agagccggct ttagccagcg attcataatc aatctcggta tccagataat
    16321 cagccggcag acagccgccg ctgggacctc cggtttgaat ggctttgagt tttttattgt
    16381 tcggaatccc gccgccgata tcatatacaa tctggcggag cgtagttccc atcggcactt
    16441 ccacaaggcc tgtattcaaa accttgccgg ccagagcaaa cacctttgtt cccttgctgg
    16501 ttttggtccc gattgaactg aaccaatcgc cgccatcgag aataataact ggtatgttag
    16561 cccatgtttc aacattgtta atcagcgtcg gcttgccgaa aagtccgctg accgacggat
    16621 acggcgggcg gggccgcggc atgccgcgca caccctcgat ggagtgaatc agggcagtct
    16681 cttcgccgca aacgaaa
//
LOCUS       DAOTBO010000064        16570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2894_length_16570_cov_5.466788, whole genome shotgun sequence.
ACCESSION   DAOTBO010000064 DAOTBO010000000
VERSION     DAOTBO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 16570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16570
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_16570_cov_5.466788"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..2390
                     /locus_tag="PLE88_08345"
     CDS             279..2390
                     /locus_tag="PLE88_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOM76509.1"
                     /translation="MEFFGFSPIDLLVIGIYLFAITYIGKRIGANIRSEEDFFLAGRS
                     LGKVFQYFLNMGTLSDANSAVRTASFAFSKGLGGVWLMLIGIFTGPYYWFMAGWFRRV
                     RLVTMAELFEERFGSRRLASVYAIVGIWLSILIMGAGYKASLRTFQAMTIKPMEKCSP
                     AERQTIAQYSRYRQLDEWYKAGRLDTGELAEYQTLDNLYKKGEIAAYASYTKPFWFYL
                     IYTLFIGLYIIMGGFRAAAVTDALQGVLILIFSFLLIPLALAKLGGWEEFTSRIPDQM
                     LFVFGSGSDEFAWNSIAALVLVTIIGITGHQGNMSLNGSARDELTARIANIGGAYTKR
                     LLTIVWALCGLFAYALYQASISDADMAWGVLSRNLLGPGLRGIMVAGILAANMSTLDA
                     VCVYLSALFVRHLYKPLIAHRSPRHYINASRAAIVVFLLLGIYVSSTTTSILHLMMAL
                     PSLNIIFGAPVLLLLFWKRLTVKAVWAEVIICSILFALLPQTLPLLGFVKNSDWLTQQ
                     TAEQTVQRSVPATARDVEAGLAGAVGEHIQKTVAVKPTAIFYDSVVREDPTHETSPMA
                     GLGRLHTELILLKLAGFDLRQASPSLLLTLRYLVAAFLPFAILIPVSLVTDNKGLEER
                     IARFYVKMKTPVDPDPAEDARRLEESYAHPTRFDHTKLFAQSNWEFCKWTKTDTCGFL
                     VSLGLTGGIILLFWGLIRWIA"
     gene            complement(2399..4186)
                     /locus_tag="PLE88_08350"
     CDS             complement(2399..4186)
                     /locus_tag="PLE88_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76510.1"
                     /translation="MPKSVSLVALVTMVLSASVVLRAVPPAFPGAEGAGRFSVGGRGG
                     SVYEVTNLSNSGPGSLVDAVSAGNRTIVFRVAGTIELGSVILRPKSYTTIAGQTAPGD
                     GICIKGRIYIGNVTDVIIRYLRVRVDEGGANSSGDAIDIDEGKNIIIDHVSASYARDE
                     TISCQDGSNNITVQWCILSEALTYEGHSYGSLIRGQYGERKTYHHNLYAHNRGRNPRP
                     GNYTPAASDPEGLYFDFRNNVVYNWAGTHPGYNADTTSISRYNFVGNVFIRGPESSGT
                     KAFKEDAKGAYAYWAGNAHGAAYSTIAVPADQWSLVTFNGFTADEIAAYKARSCELAM
                     EPVTTTSAAQALADVLEKAGASFPVRDIIDARIVRDVINGTGHSIAVTDDQPEGAWPP
                     LAGGPAPADTDHDGMPDVWETANGLDPFNPADRNGYVLHPDYTNLEVYLNAILEGMPP
                     AVDAGMDQVVWLGMSGTAGQETVYLHGSVSDDGLPGPVSVYWIQEDNGSPSVSISPAD
                     SENPAVVFTQRGVYLFRLTADDGSGAVWDTAAVIVGDDACDASHLLTGRPYSAGDLNR
                     DCIVDGMDLALFAAGWLDCTDWLTHCAGL"
     gene            complement(4206..6071)
                     /locus_tag="PLE88_08355"
     CDS             complement(4206..6071)
                     /locus_tag="PLE88_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM76511.1"
                     /translation="MKKEAAVFIVLLFAASARAAVDGFGQYTTGGAGGQVVTVSDAAQ
                     FRTYVETPNTPYVIQVSGTLELSGADSGRVRIQSNKTIRGIGDNPTIIGSLGFKNDCS
                     NVIIERLTITCPKDYTSEEDGISIKERITHVVVTRCTLYDCWDGLLDVARRSDWITVS
                     WCKFFFTYRNSNNNRVSLVGNTDSSGDEGTLHITFHHNWFGDLCMQRIPSVRYGRAHI
                     YNNYYNCPGNLYCIWSRLYAECLIENNYFKDVNNPYYNIDYDTTVKGKIGASGNILDN
                     CTGTVHPGTDAVFAPPYAYALDPAAKIPAIVQWGAGADGKEGLPPHWLFGMYGDFDGS
                     GLVDMPDLAQFARYWQAADAVADADYDGSGTVDIGELALLAHNWIQVPADTTAPEAPA
                     GLWAQGNPDGVRLDWADNAEADMAGYNIYRSAVFGSGYVKLNESLLTESCYADSAAAG
                     GTMYYYVVRAVDGSGNESPDSPAACAVSDSTANVIIQEAAAGFCAVDGKVDISGEHPG
                     FTAGGYLDTTNALGSGINWRIHAAAPGMYTFRWRFANGSTDRPARLLINGVQEIASID
                     FPSTGAWENWSQAVVQITLTAGVKDIRLEALTSGGCANIDYFCAAGEDPQIAVCP"
     gene            complement(6136..8292)
                     /locus_tag="PLE88_08360"
     CDS             complement(6136..8292)
                     /locus_tag="PLE88_08360"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOM76512.1"
                     /translation="MNAGLYSLIAAVLFCMMSGGAWAVPAFPGAQGYGAAASGGRGGE
                     VYFVTNLNDSGPGSLRDALNHNTPRTIIFRVSGTIHLQSNLDIKYPNVTIAGQTAPGQ
                     GICIANGSLYCGYDNVIIRYIRCRLGDQWPDGTPNGDDDAVWGRGGNRIILDHVSASW
                     SVDETFSFYTNSNFTAQWCMITESLRYSHHEKGPHGYGGIWGGTNSSWHHNLLAHHSS
                     RNPRIEGDVVENVDLRNNVIYNWGFNSCYGGEKASVNIVNCYYKYGPATGSGVRYRIA
                     NPSLQKDASGAAVVPHSYGQWYISGNYVYGNAAVTNDNWLGVNPEGGDAERSLCRAQT
                     PFPEAQIYPVATQSPQEAFESVLNEAGCSLYRDAVDARIVSEVRSGTASGSGQTGEGL
                     GIIDSQTAVGGWPPLETLPAPPDSDNDGMPDGWELTHGLNPAYAADRNGDRDGDGYTN
                     LEEYLNWLCTPAARADLNRNCVIDLGDFAVLAASWKTCPGQALWNPFCDIARPANGVV
                     DLSDLQVLAENWCNQPQAAQPLTKVSIIARQGKAIQYKNDSGAWISETRLKTRTSGQW
                     DPDPLLNTLDANKSYLQFDISGFGGTIVQATLTIYAITDAKSYTVSGLLDGVDEAWRM
                     DTIDWFNAPGNDTQSGSALNPAQTVLLYSVNPTAADTAASGDVTAFVCADTDGLVTFI
                     LTAGGTTYLYNVIEGAYFNAAYVPVLTLEGILTPAG"
     gene            complement(8357..8563)
                     /locus_tag="PLE88_08365"
     CDS             complement(8357..8563)
                     /locus_tag="PLE88_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76513.1"
                     /translation="MINQWAAESIPIKPIVERAVIAPVYQLKDAPKSLLCKKRDKIAQ
                     IWGFAPFPLFPEIPFSAIIYNLRG"
     gene            complement(8734..9804)
                     /locus_tag="PLE88_08370"
     CDS             complement(8734..9804)
                     /locus_tag="PLE88_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM76514.1"
                     /translation="MNILKRHAGLTDQRVPILLTVLLAGFLLSGCASQSLPSGQSSML
                     SLQGNLGVHDPVIIKEGDTYYIFATGGRAGRGFIPVKCSKDMLNWESCGYVFEQLPAW
                     APVEIPGTSGIWAPDISYFNGLYHLYYSISTFGRNNSAIGLATNKTLNPQSPDYKWVD
                     HGLVVRSTEGVDDFNAIDANIAVENSGRVWLCWGSFWGGIKMRQIDPASGMLLDTNPV
                     LYSLCSRPRFREHQTPPVEGAVEAPFLVQRGGYWYLFVSFDFCCRGPNSDYKIAVGRS
                     KKITGPYVDKDGKPMTEGGGTIVLQHGDGQWRGPGHCAVFSEAGQDYLVFHAYHFQTG
                     RSELKIAAIQWQDGWPAAPPLP"
     gene            complement(9931..11295)
                     /locus_tag="PLE88_08375"
     CDS             complement(9931..11295)
                     /locus_tag="PLE88_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM76515.1"
                     /translation="MLDWQDEFDTSHEWDQAEELAIEAHELYENGQMQKAMEKLDLAI
                     RQRPEHGQWQFNMALTLDALGEYEKAIAFYERAAELAPGDIEILNCLGVDCTRTGLYD
                     QAIDYFEQIEQIDPNFEPAYCNRIIVYTEMEQHDRAEQMFYTAQQINPDCPLCFYNIG
                     NSLFTRGQYEKAIWCWDKCASLDPAHPQIHYRIAQACWISGQGCRARQEFIAELRRNP
                     ADLDVILDFGLFLLESGDLIAAREKFTRILEFDESFTLAHFYLGEVYRIQGAEEQAFA
                     CYRRALQGDPSLVGPRFRLAELCLKRGQPCKAVSLLRDEFEQGIEDIEVLLAMGWMFL
                     RADTASDAANCFVQILNEAGPHNEAFYGLAMTLAAHADYEGALHCVEQALCIGPCRPD
                     WLLAAAWLCCKLRKWPQAEECIRKCRQLQPNRQPWESRCRRLLRFIRCSQFIDAVKAF
                     LKKP"
     gene            11639..12103
                     /gene="ribE"
                     /locus_tag="PLE88_08380"
     CDS             11639..12103
                     /gene="ribE"
                     /locus_tag="PLE88_08380"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF000812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOM76516.1"
                     /translation="MIEEIKGQLTAGKNKFAIVVSRFNEFITSKLVEGAIDTLQRHGA
                     ADSQITVVWVPGTIEITLAAKKLAESGKFAAVICLGAVIRGSTSHYDYVCQQISRGVG
                     QINYDTGVPAIFGVLTCETLEQAIERAGTKQGNAGAHAAMAAMEMANLLGRL"
     gene            12142..12594
                     /gene="nusB"
                     /locus_tag="PLE88_08385"
     CDS             12142..12594
                     /gene="nusB"
                     /locus_tag="PLE88_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOM76517.1"
                     /translation="MDRRTLARELTMQALCQLDVQGSDALPMLRIFFCDNTSDPMAIQ
                     LAEKWTLGAWEYVRACDDLIAQAAVRWKLSRLSHVDRAILRMAVYQLRYCPDIPCKVV
                     INEAIEIAKKFSTEQSPRFVNGVLDAVLKMLPRSVQSSAAEETCRRKK"
     gene            12576..13496
                     /locus_tag="PLE88_08390"
     CDS             12576..13496
                     /locus_tag="PLE88_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM76518.1"
                     /translation="MPPKKIAVLNQKGGVGKTTTVVNTAAALALKGRRVLAIDMDPQA
                     HLTIHLGADRDAQAAGTYEVLVEGLALGDAVRPVRTNLWLLGAGIDLVGAESELVNEV
                     GREIILRQALQAVREQFDYVLIDCPPSLGLLTLNALAAAEEVIIPIQPHFLALQGFSR
                     LLQTVTLVHSRINPALRVSSILMCMFDSRTSLSNEVCQDIERFLSSARGSTHPWADAA
                     VIPVHIRRNIKLAEAPSHGQTIFEYEPSCNGAADYEAVADFIDRSFLLPAAAAEQPST
                     QAAAASIEESATPVHSVETLNQQTPIGGKE"
     gene            complement(13681..14382)
                     /locus_tag="PLE88_08395"
     CDS             complement(13681..14382)
                     /locus_tag="PLE88_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOM76519.1"
                     /translation="MLQQRHPENQLPLTRIQRLIGTYMLQSKRTKPSAYIRMRADLTE
                     LAALRKQFCRTSGIRASTNDFFIYAIAQAVKAFPLMAASIDAAGTHRIIAEHIGIGLA
                     VAAPQGLVVPVLKDMETKTLAKTAAESETLLKKARSGKLMPADFDGATMVLTGLGMYG
                     IESFYAISPPSATGIISIGSIDEAVMPAGDSFQIRKMMYVAMAFDQRLAGEFYTAAFL
                     RKVVDLLQNPAVLIN"
     gene            14530..14652
                     /locus_tag="PLE88_08400"
                     /pseudo
     CDS             14530..14652
                     /locus_tag="PLE88_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
     gene            14755..15423
                     /locus_tag="PLE88_08405"
     CDS             14755..15423
                     /locus_tag="PLE88_08405"
                     /inference="COORDINATES: protein motif:HMM:NF022620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HOM76520.1"
                     /translation="MKPIPSQLCTLLAALAAITAVAGCQKVYYGTMEKFGVHKRDILV
                     SNVAQARDAQNEAKAQFASALEEFSAVANFQGGKLEEKYKTLNAEYQRCQKKAEAVKE
                     RIGDVKRVAKALFEEWEKELDQYSSDALRKASQAKLEQTQQRYKSLIGAMERAESKIA
                     PVLAAFGDQVLFLKHNLNAQAVASLQSELSTMESEIGTLIREMERSINEADAFIQEMT
                     QQES"
     gene            complement(15554..15874)
                     /locus_tag="PLE88_08410"
     CDS             complement(15554..15874)
                     /locus_tag="PLE88_08410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM76521.1"
                     /translation="MESTIVEQRQDVRSELSWPVSIWLPQANRFFNGRSVNVSRGGAF
                     LSVPMTTPVCPGQEIEINFPRTPTLARQKGQYARIKNGRVLRVERQRMLQAGSIGIAV
                     QFDK"
     gene            complement(16119..>16570)
                     /locus_tag="PLE88_08415"
     CDS             complement(16119..>16570)
                     /locus_tag="PLE88_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOM76522.1"
                     /translation="ELTPIQQSQPIDFYPSSLTADDIPVRRREGFKSLLSLRQLTELE
                     RNPGTRRTDLAELAVQKHSRITDPLINLIMLMVALPVLVCRDPKAMKNAIIISFLTTL
                     SCFVTVFICKLFAAEVILGQLRPALWTWAPIFIFFPIALIEIDSMRT"
BASE COUNT         3754 a         4378 c         4709 g         3729 t
ORIGIN      
        1 tttacggatt tttgaagata gcaagatagt gcatgcagaa ttgggaccat ctggatagtt
       61 tggatagaca attaatcgcc tgccttgtct tggagttttg aggtcaagca acgtttaaaa
      121 aagatgtctg ctgcacagct aatcggcttt tttatctatt tgcagtagaa atacggacaa
      181 tagattgata tattaacggc acagtaagtc ttcaaaaggc aacagatttt tcgcattgcg
      241 gctgacaaca aaccgctgtt ggggagtaac aggaacggat ggaatttttt ggttttagtc
      301 cgatcgacct gctggtgata ggcatttatt tgtttgcgat tacctatatc ggcaagcgga
      361 ttggggctaa tatccgttcg gaagaagatt tttttctggc cggccggtcg ctgggcaagg
      421 tttttcagta ctttctgaat atgggcacgc tcagcgacgc caacagtgcc gtccgcacag
      481 ccagtttcgc tttcagcaaa gggctgggcg gggtttggct gatgctgata ggaatcttca
      541 cagggccgta ctactggttt atggccggct ggtttcgccg ggtgcgtctg gtcacgatgg
      601 ccgagctgtt tgaggaacgg tttggcagcc gccggctggc atcggtatat gcgattgtcg
      661 gcatctggct gagcattctg attatgggag ccggctacaa ggcctcgctg cggacctttc
      721 aggcgatgac catcaagccg atggaaaaat gcagccctgc cgagcgtcag actattgccc
      781 aatacagccg ctaccggcag ctcgatgaat ggtacaaggc cggccggctg gacactggcg
      841 aattggccga gtatcagacg ctggacaatc tgtacaaaaa gggggagatt gcagcgtatg
      901 cctcctacac caagccgttc tggttctacc tgatttatac gctgtttatc ggcctgtata
      961 tcattatggg cggttttcgg gcggcggcgg ttaccgatgc cctgcaggga gtactgattc
     1021 tgattttttc ctttctgctg ataccgctgg ctctggccaa actgggcggc tgggaggagt
     1081 ttaccagccg gattcccgac cagatgctgt ttgtgttcgg ctcgggcagc gacgagtttg
     1141 catggaactc gattgcggcg ctggtgctgg tgacgattat cggcatcacg gggcatcagg
     1201 gcaatatgtc gctcaacggc tcggcgcggg atgagctgac cgcccgcatc gccaacatcg
     1261 gcggggctta caccaagcgg ctgctgacga ttgtgtgggc gctgtgcggg ctgtttgcct
     1321 acgcgctgta tcaggcatcg atttcggatg cggatatggc atggggggtg ctcagccgca
     1381 atctgctggg gccgggactg cgggggatca tggtcgccgg catactggcg gccaatatgt
     1441 ccacgctgga tgcggtgtgc gtgtatctgt cggcgctgtt tgtgcggcat ttgtacaagc
     1501 cgctgatagc ccatcgctcg ccgcggcatt atatcaacgc ctcacgggcg gcgattgtcg
     1561 tgtttctgct tctgggcatt tatgtctcgt ccacgacaac cagcatcctc catttgatga
     1621 tggcgctgcc gtcgctgaat atcatattcg gtgcgccggt gctgctgctg ctgttctgga
     1681 agcggctgac ggtcaaggcg gtttgggcag aggtgattat ctgttcgatt ctgtttgcgc
     1741 ttctgccgca gacgctgccg ctgctgggat ttgtaaaaaa ttccgactgg ctgacacagc
     1801 agacggccga gcaaacggtg cagcggagcg tgccggcaac ggcgcgggat gtggaggccg
     1861 gactggccgg cgcggtcggg gagcatattc agaaaacggt ggcggtcaag ccgacggcga
     1921 ttttctacga ttcggttgtg cgggaagatc cgacgcatga gacctcgccg atggcgggtc
     1981 tgggacgcct gcataccgag ttgattctgc tgaagctggc cggctttgac ctgcggcagg
     2041 ccagcccgtc gctgctgctg accctgcggt atctggtggc ggcctttctg ccgtttgcga
     2101 ttctgatacc ggtcagcttg gtcaccgaca acaagggact ggaagagcgg atagcccgat
     2161 tttatgtgaa aatgaagacg cccgtcgacc ccgatccggc cgaagacgcc cgccggctgg
     2221 aagaaagcta cgcccatccg acccgttttg accacaccaa gctgtttgcc cagtccaact
     2281 gggaattctg caaatggacc aaaaccgaca cctgcggctt tttggtttcg ctgggcttga
     2341 ccggcgggat tatcctgctg ttctgggggc tgatacggtg gattgcctga gccgctgctt
     2401 acaagcccgc acagtgcgtc agccagtcgg tgcagtccag ccagccggca gcgaacaaag
     2461 ccagatccat tccatccact atgcagtccc gattcaaatc gccggcgctg tacggccggc
     2521 cggtcagcag atgcgacgca tcacaggcat cgtcgccgac aataacggca gccgtatccc
     2581 aaaccgctcc ggaaccgtca tcggccgtca gccggaacag atacacgccg cgctgggtga
     2641 aaacgacagc cggattttct gaatcggcag gactgatgct gacagagggg ctgccgttat
     2701 cttcctgaat ccagtataca gacaccggac cgggcaggcc gtcatcggag acactgccgt
     2761 gcagatacac cgtttcctga ccggctgtgc cgctcattcc cagccagaca acctgatcca
     2821 tgccggcatc gacagccggc ggcattccct ccagaatcgc attgagatag acttccagat
     2881 tggtatagtc cggatggagc acatagccgt tgcggtcggc aggattgaac ggatcaaggc
     2941 cgttggccgt ttcccagaca tccggcattc catcgtggtc cgtgtcggcc ggggcaggac
     3001 cgccggccag cggcggccat gcgccttcgg gctggtcatc ggtaacggca atcgaatggc
     3061 ctgttccgtt gatgacgtcc cggacgatgc gggcatcgat gatatcgcgc acggggaagg
     3121 aagcgccggc cttttccagc acatccgcca gcgcttgggc ggcagaggtg gtggttaccg
     3181 gctccatcgc cagctcgcag gagcgggctt tgtaggcggc gatttcatcg gcggtaaatc
     3241 cgttgaaggt taccagcgac cattggtctg cgggcacagc aatggtgctg taagcggcgc
     3301 cgtgggcatt gccggcccag taggcatagg cgcctttggc atcttcctta aaggccttgg
     3361 tgccgctgga ttcaggtccc cggataaaga cattgccgac aaaattgtag cggcttatcg
     3421 aggtggtgtc ggcattgtag cccggatgcg tgccggccca gttatagacg acattgttgc
     3481 ggaaatcaaa atacagtcct tcgggatcgc tggcggcagg ggtatagttg cccgggcggg
     3541 ggttgcggcc gcggttatgg gcgtagaggt tgtggtggta ggttttgcgc tcgccgtact
     3601 ggccgcgaat cagcgagccg tagctgtggc cttcataggt cagggcctcg ctgaggatgc
     3661 accactggac cgtgatgttg ttggagccgt cctgacagga aatggtttcg tcgcgggcgt
     3721 aggaggcgct gacatggtca atgatgatgt ttttgccttc gtcgatatcg atggcatcgc
     3781 cgctggaatt ggcgccgcct tcatcgacgc gcactcgcag ataacggata atcacatcgg
     3841 ttacattgcc gatgtagatg cgtcccttga tgcagatgcc gtcgccgggt gcggtctggc
     3901 cggcaatggt ggtataggat ttgggccgca gaatcacact gcccagctca attgtgccgg
     3961 cgacacgaaa cacaatcgtc cggttgcctg cactgacggc atcgacaagc gagccgggcc
     4021 cgctgttgga caggttggtg acttcataga cgctgccgcc gcgtccgccg acggaaaacc
     4081 ggccggctcc ctcagcgccc ggaaaagccg gcggcacggc gcggaggaca acggatgctg
     4141 acagaaccat cgtgaccaat gcaaccagcg atacagactt tggcatacga caaacctttc
     4201 tcccgctacg ggcaaacggc tatttgagga tcttccccgg cagcacagaa ataatctata
     4261 ttggcacatc cgccgctggt cagtgcttcc agacggatat ccttgacgcc ggctgtcagc
     4321 gttatctgca caacagcttg gctccagttt tcccacgccc cagtggacgg gaaatcgatg
     4381 ctggctattt cctgcacgcc gttgattaaa agccgcgcag ggcggtcggt tgagccgttg
     4441 gcaaaccgcc agcggaaggt atacatcccc ggtgctgcgg catggatgcg ccagttgatg
     4501 ccgctgccga gggcattggt ggtatccaga tagccgcctg cggtaaagcc gggatgttct
     4561 ccgctgatat cgaccttgcc gtcaacggcg caaaatcctg ctgcggcttc ctgaatgatg
     4621 acattggccg tcgaatcgct taccgcacag gccgccggcg aatccggcga ttcattgccg
     4681 ctgccgtcca ccgcccggac gacatagtaa tacattgttc cgccggcagc cgcagaatcg
     4741 gcatagcagg actccgtcag caggctttca ttgagcttga catagccgct gccgaacacg
     4801 gcggaacggt agatgttgta gccggccata tctgcttccg cattatccgc ccagtccagc
     4861 cggacgccgt cgggattgcc ctgcgcccac aggccggcgg gggcttcggg agccgtggtg
     4921 tcagccggca cttgtatcca gttatgggcc aacagggcca gctcgcctat gtccaccgtc
     4981 ccgctgccgt cgtaatcggc atccgcaaca gcgtctgccg cctgccagta tcgggcaaac
     5041 tgcgccaaat ccggcatatc aaccagcccg ctgccgtcaa aatccccata catcccaaac
     5101 agccaatgcg gcggcaggcc ctctttgccg tcggcgccgg cgccccactg gacgatggcc
     5161 ggaatcttgg cggccggatc cagcgcatag gcatagggcg gggcaaacac agcatccgtg
     5221 ccgggatgaa ccgtgccggt gcagttgtcc aaaatgttgc ccgaggcgcc gattttgcct
     5281 ttgaccgttg tgtcgtaatc gatgttgtaa tagggattgt tcacatcctt gaaatagttg
     5341 ttttcaatca ggcattcggc atagaggcgg gaccagatgc agtacagatt gcccggacag
     5401 ttgtagtagt tgttgtagat atgggcgcgt ccgtagcgga cggagggaat gcgctgcatg
     5461 cacagatcgc cgaaccagtt gtgatggaat gtgatgtgca gcgtgccttc gtcgccgctg
     5521 ctgtcggtgt tgccgaccag actgacacgg ttgttgttgc tgttgcggta ggtgaaaaag
     5581 aatttgcacc acgacacggt aatccaatcg gagcggcgtg ccacatccag cagtccatcc
     5641 cagcagtcgt aaagggtaca gcgggtgacg acgacatggg taatgcgttc cttgatgctg
     5701 atgccgtctt cctcgctggt gtagtctttg gggcaggtga tcgtcaggcg ttcgataatc
     5761 acattggagc agtcattctt aaagcccaga ctgccaataa tggtcggatt gtcaccgatg
     5821 ccgcgaattg ttttgttgga ttgaatgcgg acgcggccgc tgtcggcgcc ggacagttcg
     5881 agcgtgccgg agacctgaat aacatagggt gtattgggag tttccacata ggtccgaaac
     5941 tgtgcagcat ccgagaccgt aaccacctgc cctcctgccc cgccggtggt gtattggccg
     6001 aagccgtcca ctgccgccct tgccgatgcg gcaaacagca gaacaataaa gactgccgct
     6061 tcttttttca ttctaatcct cccggtcaaa cattacggat catttcggat tgcagcagac
     6121 ctctgctgcg gccggctatc ccgcaggcgt caggataccc tccaaggtta aaacgggaac
     6181 atacgctgcg ttgaagtaag ccccctcgat cacattatac agataggttg tgccgccggc
     6241 ggtcaggata aatgttacca gtccgtcggt gtcagcacag acaaacgccg ttacatcgcc
     6301 gctggcggcg gtatcggctg ccgtcggatt gacactgtac aacaagacag tctgggccgg
     6361 attcaaggcc gagccgctct gggtgtcgtt gcccggggcg ttgaaccagt ctatcgtgtc
     6421 catccgccat gcttcgtcca caccatccag caggccgctg acagtgtagg atttggcatc
     6481 ggtgatggca taaatcgtca gcgtcgcctg aacaatagtg ccgccgaagc cggagatatc
     6541 aaactgcaga tacgatttgt tggcatccag cgtattgagc agaggatccg gatcccactg
     6601 gccggaggtg cgtgttttaa ggcgggtttc ggaaatccat gctcccgaat catttttgta
     6661 ctggatggct tttccctgac gggcgatgat gctgactttg gtcagcggct gagcggcttg
     6721 cggctggttg caccagtttt cggccagcac ctgcaggtcc gacagatcca cgacgccgtt
     6781 tgccggccgg gcaatatcgc agaaaggatt ccaaagggcc tgccccgggc aggttttcca
     6841 agatgccgcc agaacagcaa agtcgccgag gtcgataaca cagttgcggt ttaaatccgc
     6901 ccgtgccgcc ggcgtacaca gccagttgag gtattcctcg agattggtat agccgtcgcc
     6961 gtcgcggtcg ccgttgcggt ctgccgcata ggcaggattg agcccgtgcg tcagttccca
     7021 tccgtcgggc atgccgtcgt tatcgctgtc cggcggcgcc ggcagcgtct ccagcggcgg
     7081 ccagccgccg acagccgttt gggaatcaat aatgcccagc ccctctccgg tctggccgct
     7141 gccgctagcc gtaccgcttc ggacctcgct gacaatgcgg gcatccaccg catcgcgata
     7201 cagactgcat ccggcctcat tcagaaccga ttcaaaggcc tcctgcgggg attgcgtggc
     7261 taccggatag atttgggctt cgggaaaagg cgtctgggcc ctgcacaagc tccgttcggc
     7321 atcgccgcct tcgggattta cacccagcca attgtcattg gttacggccg catttccata
     7381 gacataattg ccgctgatat accattgccc gtagctgtgc ggcacgacgg ctgcgcccga
     7441 agcatctttt tgaagactgg gattggcaat tcggtatcgc acaccggagc cggtagcagg
     7501 gccgtattta taatagcagt tcacaatgtt gacggatgct ttctcgcccc cgtagcagct
     7561 gttaaagccc cagttgtaaa tgacattgtt gcgcaaatct acattctcga ccacatcgcc
     7621 ttcaatgcgg ggattgcggg agctgtggtg ggccagcaga ttgtgatgcc aggagctgtt
     7681 ggttccgccc cagatgccgc cgtagccgtg cggccccttt tcgtgatgcg aataccgaag
     7741 actttccgtt atcatacacc actgggcagt aaaattgctg ttggtataaa aagagaatgt
     7801 ttcatccaca gaccagctgg ccgagacgtg gtcgagaata atccgattgc cgccgcggcc
     7861 ccacacagca tcatcatcgc cgtttggagt gccgtccggc cattgatcgc ccagacggca
     7921 gcggatatag cggattatca cattatcata gccgcaatat aaggaaccat tggcaatgca
     7981 gatgccctgc cccggagctg tctggccggc gatggttaca ttggggtatt tgatatccag
     8041 attcgattgg agatgaattg ttccggaaac acgaaagata atcgtccgcg gggtattatg
     8101 attgagagca tcacgcaggg agccggggcc ggaatcgttc aaattggtaa caaaataaac
     8161 ctctccgccc cgtccgccgg aagccgcggc gccgtagcct tgggcgccgg gaaacgctgg
     8221 aaccgcccaa gccccccctg acatcatgca gaataagaca gccgcaatta aggaatacaa
     8281 acccgcattc attccaacac cctcgccgca tccgcttggc agtaataaca aatcgggaga
     8341 ttttcacaca ctctatttac ccccgtaaat tatatattat agcagaaaat gggatttcag
     8401 ggaacagtgg aaatggtgca aagccccaaa tttgcgcaat tttatctctt tttttgcaca
     8461 aaagtgattt tggagcatct ttcagctgat acaccggtgc gatgactgcc ctttcaacaa
     8521 ttggcttaat aggaatcgat tcagcagccc attggtttat caaacggtta atcctgtcaa
     8581 gaagcagttt ttacaggtct gatgaccgcg cctgactgcg tgcgatagtt ggaacggagt
     8641 cccccccttt gtccgctgtg ccgttttaat aggctgctaa ccgccggcaa attacggcat
     8701 gcctgctgtt tagataaatt cagccgccat tcatcacggc aaaggcggag ccgccggcca
     8761 gccgtcctgc cattggatag ccgcgatttt cagctcagac cgtccggttt ggaaatggta
     8821 ggcatggaaa acaaggtaat cttggccggc ttcgctgaaa acagcacaat ggcccggtcc
     8881 tcgccattgc ccgtcgccgt gctgaaggac aatcgtgccg ccgccttcgg tcatcggctt
     8941 gccgtctttg tcaacatagg ggccggtgat ttttttcgac cggccgacgg caatcttata
     9001 atcgctgttg gggccgcggc agcagaaatc aaacgacaca aataaatacc aatacccgcc
     9061 ccgctggaca aggaacgggg cttcgacggc accttctacc ggcggggttt gatgctctcg
     9121 gaagcgcggg cggctgcaga gcgaataaag cacaggattg gtgtccagca gcataccgga
     9181 ggccgggtcg atttggcgca tcttgatgcc gccccagaag ctgccccagc acagccatac
     9241 cctgccgctg ttttcaacgg caatattggc atcgatggca ttgaaatcat ccacgccttc
     9301 ggttgaccga acaacaagtc catgatcgac ccacttgtaa tcagggcttt ggggattgag
     9361 tgttttattg gtggccaatc cgatggcaga gttgttgcgg ccgaaggtgg aaattgaata
     9421 gtagagatga tacaggccgt tgaaatagga aatatcgggc gcccagatgc cgctggttcc
     9481 ggggatttcg accggcgccc aagcaggcag ctgttcaaag acatagccgc acgattccca
     9541 attgagcata tccttggagc atttgacagg gataaatccc ctgccggccc tgccgccggt
     9601 ggcaaagatg tagtaggtat cgccttcttt aatgataacc ggatcgtgta cccccagatt
     9661 gccctgcagg gaaagcatag aggattgtcc ggacggcagg gattggctcg cacagccgct
     9721 cagcagaaat cctgcaagca ggacggtcag cagaatagga actcgctggt ctgtcaggcc
     9781 ggcatgcctt ttcagtatgt tcattacaaa actccttaat cgaggatcga tagaacctgt
     9841 tcgatgcctt attaataacc gcaaggaagt gtcaatttca acaaaaatat ccgctcattc
     9901 cggcggaagc gagaggatgc caaaggggat ttacggcttt ttcaagaagg cttttacagc
     9961 gtcgatgaat tggctgcagc ggatgaagcg gagaaggcgc cggcatcggg attcccacgg
    10021 ctgacggttg ggctgcaact ggcggcactt tcggatgcat tcttcggctt gcggccactt
    10081 gcgcagcttg cagcacagcc acgcggcagc cagaagccaa tccggccggc acggaccgat
    10141 gcacagtgct tgttcgacac agtggagggc gccctcgtaa tcggcgtggg ctgccaaggt
    10201 cattgccaat ccgtaaaagg cttcattatg cggaccggct tcattgagaa tctgcacgaa
    10261 gcagttggcg gcatccgaag ccgtgtcggc ccgcaaaaac atccagccca ttgccagcag
    10321 aacctcaatg tcttcgatgc cctgctcaaa ctcgtcccgc aggagggaaa ccgccttgca
    10381 gggctgcccg cgcttcaggc agagttcggc caatcgaaat ctcggaccga caagcgatgg
    10441 atcgccctgc agggcgcggc gatagcaggc aaacgcctgt tcctccgccc cctgtatgcg
    10501 atagacctcc cccagataaa aatgagcaag ggtaaatgat tcgtcaaatt cgagaatccg
    10561 ggtaaatttt tctctggcgg caatgaggtc gccgctttca agcaggaaca gcccaaaatc
    10621 gagaatcaca tccaaatccg ccggattgcg gcgcagttca gcaatgaatt cctgccgtgc
    10681 gcggcatccc tgcccgctga tccagcaggc ttgagcaatg cggtaatgaa tctgcggatg
    10741 ggcggggtcg agactggcgc atttgtccca gcaccagatt gccttttcgt attggccgcg
    10801 tgtaaaaaga gagttgccga tgttgtaaaa gcacagcggg cagtccggat taatctgctg
    10861 ggcggtgtaa aacatctgtt ccgcccggtc gtgctgctcc atttctgtat agacaatgat
    10921 gcggttgcag taggccggtt caaaattggg atcaatctgc tcaatctgtt cgaaatagtc
    10981 tatcgcctga tcatagagac cggttcgggt gcagtccaca cccaaacaat ttaatatctc
    11041 gatatcgccg ggagccaatt cggcagcccg ctcatagaag gcgatggcct tttcatattc
    11101 tcccagcgca tccagcgtca aagccatatt aaactgccat tggccatgtt ccggtctttg
    11161 gcggatggct aagtcgagct tttccatcgc tttctgcatt tggccgtttt catacagctc
    11221 atgggcttct atggccagtt cttcggcctg atcccactca tgcgaggtat caaattcatc
    11281 ctgccaatcc agcattcaca ctccgcagct aattttactt cttcattacg gactcgacag
    11341 gggatatatc gtcataaaga gttcctgtct tagccggtac agcagatttt gaagccattt
    11401 tgaagcggga aagttttttc tggctttcta tgccttaacc ttttataatt taacggctta
    11461 aataaacgct gcagcgatgg aaagccagcg gcaggcaaat tctggatgac ttttgacggc
    11521 tcgtccgata agatagtagg tgatgcatat gggtgcgaaa ctctttacag agcaaggcaa
    11581 taggattgag gcctgctgcc ggaaggcatt aacaacagct ttttttggag cagaatagat
    11641 gattgaggaa atcaaaggtc aattaaccgc cggaaagaat aaatttgcga tcgttgtcag
    11701 ccggtttaat gagtttatta ccagcaaact ggttgagggt gcgatagata cccttcagcg
    11761 gcatggagcg gctgacagcc agattacggt cgtctgggtg ccgggaacaa ttgaaataac
    11821 gctggcagcc aagaaactgg ctgaaagcgg caagtttgcg gctgtcattt gtctgggagc
    11881 ggttatcagg ggctccacca gccactacga ctatgtctgc cagcagattt cgcgcggggt
    11941 cgggcaaatc aattacgata cgggcgttcc ggcgattttc ggagtattga cctgcgagac
    12001 actggaacag gccatcgaac gtgcgggcac caaacagggc aatgccggcg ctcatgccgc
    12061 aatggcagcg atggagatgg ccaatcttct gggccgtctg taaccggttg atctggcaag
    12121 tattgtgatg aaggaacggc tgtggaccgc agaacattag ctcgagaact gaccatgcag
    12181 gccctctgcc agctggatgt gcagggcagc gatgcgctgc cgatgctgcg gattttcttt
    12241 tgcgacaata ccagcgatcc gatggctatc cagcttgcgg aaaaatggac gctgggcgct
    12301 tgggagtatg tgcgggcctg cgatgatttg attgcacagg cagcggtgcg ctggaaatta
    12361 tcgcggctgt cgcatgtcga tcgggcgatt ctgcggatgg ccgtatatca gcttcggtac
    12421 tgtcccgata ttccctgcaa agttgtcatc aacgaggcca tcgaaatcgc taaaaaattc
    12481 agcacggaac aatcgccgcg ttttgtcaat ggagttctgg atgcggtgct gaaaatgctg
    12541 ccccgttctg tgcagtcttc agctgcggag gaaacatgcc gccgaaaaaa atagcggtgc
    12601 tgaatcaaaa gggcggagtg ggcaaaacca caacggtcgt taacacagct gccgcgctgg
    12661 ccctcaaagg gcggcgcgtg ctggcgatcg atatggaccc acaggcccac ttgaccattc
    12721 atctgggcgc cgaccgcgat gcccaagccg cggggactta tgaggtgctg gttgaagggc
    12781 ttgcgctggg cgatgctgtt cggccggtgc gaaccaatct gtggcttctg ggtgccggca
    12841 ttgatctggt cggggcggaa tccgaattgg tcaatgaagt cggacgggaa atcattctgc
    12901 ggcaggcgct gcaagcggtt cgcgaacaat ttgactatgt acttatcgac tgcccgccgt
    12961 cgctgggcct gctgaccctc aatgctctgg ccgctgcgga agaggtgatt attccgatac
    13021 agccgcattt tcttgctctg cagggattca gccgcctgct gcagacagta acgcttgtgc
    13081 acagccgcat caatccggca ctgcgggtgt cctcgatttt gatgtgcatg tttgacagcc
    13141 gaaccagctt atccaatgaa gtatgtcagg atattgaacg gtttttatcc agtgcgcggg
    13201 gcagcaccca tccgtgggcg gatgcggctg tgattccggt gcatatccgg cgaaacatca
    13261 agctggcaga agcccccagt cacggtcaga caatttttga atatgagccc agctgcaacg
    13321 gtgcagccga ttacgaagcc gtggctgatt tcatcgaccg gtcgtttttg cttccggcag
    13381 ccgcagcaga acagcccagt acacaagctg ccgctgcatc catcgaagaa tccgccacac
    13441 cggtgcattc tgttgagacg ctcaaccagc aaactccaat cgggggaaaa gagtaaaaag
    13501 aaaaggtcgc ttcgtggatg aaaggaagca accttttata ttaaagaccg ctcaggcgaa
    13561 cgatcttgtc tttctgaatg ttcaattatg cgccggattt gtgcgctgtg tcaacccttt
    13621 ttttcaaaaa gctgcaaaat ttttcttcct aaaaagattg cacgctttgt gaagaatttc
    13681 tcagtttatc agcacagccg gattctgcag cagatccaca acttttcgca agaaggcagc
    13741 cgtgtaaaac tcccctgcca gacgctggtc gaaagccatt gccacataca tcatcttacg
    13801 tatctgaaag ctgtccccag caggcatcac ggcttcatca atgctgccga tggagataat
    13861 ccccgtcgca gagggagggc tgatagcata aaatgactct atgccgtaca tccccagccc
    13921 ggtcaaaacc atcgtagctc catcaaaatc cgcgggcata agcttacccg agcgggcttt
    13981 tttaagcagc gtttcgcttt cggcagcggt tttggcaagg gtttttgtct ccatatcctt
    14041 cagaaccggc acaaccagtc cctgcggcgc tgccacggcc agcccgatac cgatgtgttc
    14101 ggcaataatg cggtgcgttc cggcggcatc aatggaagcg gccatcaggg gaaatgcttt
    14161 gacggcctga gcaatggcat agatgaagaa atcattggtg gaagcccgaa tgcccgatgt
    14221 acggcaaaac tgcttgcgca atgccgccag ctcggttaaa tccgcccgca tgcggatata
    14281 ggcgctgggc ttggtccgct tggactgaag catatacgtg ccgatgagcc tttgtatccg
    14341 cgtcagcggg agctggtttt ccggatgcct ctgctgcaac attgtccttt ctctttctgc
    14401 ttttttatgc aatttggaag ccttattgta tgcttgacag gacaacaatt caagcataaa
    14461 taccattgcc gggacgcaag ccgaagatta taataagccg caaaaaacag gctttataaa
    14521 ggagagaaga tgcgatactt tctggcgatt gttctgccgc cgctggcggt ccttttatgc
    14581 ggcaagccga tacaatttct gctgaatatt ctgctgacgc tgctggggtg gattccgggt
    14641 gtcgtgcatg ccattctggt tgttcacagc cacttggatg ataaacggac aaaaaaaatt
    14701 attgaggccc tgaaaaacaa ataaaccgtc cgaaacagac agaaagggga aggtatgaag
    14761 ccgattccaa gccaactttg cacgcttctg gcagcccttg cggcaataac cgctgttgcc
    14821 ggctgtcaga aagtgtatta tggaacgatg gaaaaattcg gggtgcataa gcgggatata
    14881 ctggtcagca atgttgccca agcccgcgat gcacagaatg aagccaaagc ccagttcgct
    14941 tcggcactgg aagagttcag tgcggtggca aactttcagg ggggcaagct ggaagaaaaa
    15001 tacaagacgc tgaatgccga ataccagcgc tgccagaaga aggccgaagc cgtaaaggaa
    15061 cggattgggg atgtcaagcg ggtcgccaaa gccctgtttg aggaatggga aaaagagctg
    15121 gatcagtaca gcagcgacgc cctccggaaa gccagtcagg caaaactgga gcagacccag
    15181 cagcgctata agagcctgat aggagcaatg gagcgggctg aatcgaaaat agcgccggtg
    15241 ctggcggctt tcggcgacca agtgctcttc ctcaaacaca acctcaatgc acaggcggtg
    15301 gcttcgctgc agagtgaatt aagcaccatg gaaagtgaaa tcggcacatt gattcgggaa
    15361 atggagcgtt cgattaacga agcggatgcc tttattcagg aaatgaccca gcaggaatca
    15421 taaaccaccg ggatacagcg cggccggcat gataaaaaat catgccggcc tttcaatata
    15481 ttgacccgca cttaaacggc gcgggacccg ttttgacttg ccaaaaaaaa gcagatatgt
    15541 cggcggtttg caactattta tcaaactgca cagcaatacc gatggaaccg gcctgcagca
    15601 tacgctgacg ttcaaccctt aaaacacgtc catttttaat gcgggcatac tgtcccttct
    15661 gacgggccag agtgggcgta cgggggaagt taatctcgat ttcctgtccg ggacagacag
    15721 gagtggtcat aggaacagaa aggaatgccc cgcccctgct gacattaaca cttcggccgt
    15781 tgaaaaaacg attggcctgt ggaagccaaa tactgaccgg ccagcttaac tccgaacgga
    15841 catcttgacg ctgttcgaca atcgtgctct ccataattcc attctccgta aaagtggtga
    15901 actataacgt catagatatc ttatcgtcaa tgttaaactg gttaatttag tatatataga
    15961 taaaataatt tattttttag aaaaaattga agttaagttc agatttggat aaatgcagag
    16021 atattatcgg tccactatct tggagataaa agtttgtcgc cggacaacta ccgtccgata
    16081 actattatct gctacaaaag gtgaacagaa ggagggcttt atgttctcat cgaatcgatt
    16141 tcaatcaggg ctatgggaaa gaaaataaaa atgggcgccc atgtccagag agccggccgc
    16201 aattgaccca aaattacctc tgcagcaaac agcttgcaga taaaaaccgt aacaaagcag
    16261 ctcaaggtcg tcaaaaaact gataataatt gcatttttca ttgctttggg atcccggcag
    16321 accagcacag gcaaggcgac catcagcata ataagattaa tcagcggatc ggtaatgcgg
    16381 ctgtgtttct ggactgccag ttcggccaga tcggttcggc gtgtgccggg atttctttcc
    16441 aattcggtca gctgacgcag actgagcaga gatttgaaac cttctcggcg gcggacagga
    16501 atatcgtcgg cagtcagact gctggggtaa aaatcgatcg gctggctttg ctgaatcggg
    16561 gttagttcct
//
LOCUS       DAOTBO010000065        16525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_2909_length_16525_cov_5.990832, whole genome shotgun sequence.
ACCESSION   DAOTBO010000065 DAOTBO010000000
VERSION     DAOTBO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16525
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2909_length_16525_cov_5.990832"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLE88_08420"
     CDS             <1..638
                     /locus_tag="PLE88_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76523.1"
                     /translation="VSSIAEILADMNIADAKVTSAGGLDTLIKQFKAARRNIAQGLAS
                     IERLEEQTARLDEQFFEIDKQMRSGLMTREVTQGGSSVYQRRSRNGLPSDYNRSLRDS
                     AGDSLPSNQTSASGRIRSPRSESITTIEPAANIEVVRSQLIASLRNLTAEYRRVINLA
                     KRWPGGLPAQVSIDQLQTNMNSAAAQLDSLYRYQPIYQSQPIQPSAGRLIR"
     gene            815..2461
                     /locus_tag="PLE88_08425"
     CDS             815..2461
                     /locus_tag="PLE88_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOM76524.1"
                     /translation="MIHSSIIKYLWVLPLLFGLIGCTQPAPTTNPAAAVDPRSTIGSI
                     SAYYANESVAVRGIGIVAGLAGTGSSECPPGIREELEKYIWKQMPNIAVNPRLFIESQ
                     NTAVVEIIGVIPPYATRQTAFDVIVRPLSSTQTTSLNGGYLYTAELKELSRLTNVGQF
                     AQYSKTLAAAEGPIFTKLSQDASDRKWYVLGGGRPAEDGFVKLILKTPDFVAANAIRN
                     RINERFGPKTAVPVSAGECNLFFPAKYLDQKERFLKMVQTLILADNPDIRNDYMQTLL
                     DRLVRDSDKESAEIALEGIGKPVLDSLESLLEHNDPAVQFYAARCMLNIGDSRPLPVL
                     RKIILEPASPYRIAAIRAVGQSAKRSDARPILLNALHDNDIQVRLAAYEMLGPMDSPE
                     ISRTLVAKGSFAIDRVLCSGPKMIYVYQQLSPRIVLFGSPLHCEKDIFIQSDDEMVTI
                     NARSGDKYITVSRKHPSRPRVVGPLSCSYELSSLIQTLGEVPDVKGTSAPRPGLNIPY
                     ADIIRILQKMCRQDAIKAQFIAGPEPEKNPLLQNLPVIGR"
     gene            2501..6067
                     /gene="smc"
                     /locus_tag="PLE88_08430"
     CDS             2501..6067
                     /gene="smc"
                     /locus_tag="PLE88_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOM76525.1"
                     /translation="MKLEKIIINGFKSFADKTEFSISHPITAIVGPNGCGKSNVVDAL
                     KWVLGNQSPKSLRSGQMSDVIFSGSSSRKPSGMAEVTLHFSDVHGLELEQDELQISRR
                     LFRSGESDYLINGKSCRLKDIRELFMDTGIGVSAYSIIEQGQIDQLLKSSTQDRRIIF
                     EEAAGISKYKAHKKEALRKLERTEQNLLRLADIVNEVQKQLRSIKLQAGKARSYLEYS
                     EKLKELRVNYSLAEYDRIVTQQREKNTVLADWKAQFGQTAADLARCDAAVSQLTHAIM
                     ETDSQINRWDGILISARSRIEQHHDRIGFLNNRLHELQDRKKNAADQIAKLMEQTCQL
                     ASQIKACQKILQEVEQLSETTASRLHQAEQVISTITAQCAELEAQLDDEKSGIIDIVR
                     RTAQLHNEIESLTSYRSTLSGRKTRLSGKAEETQHQLSQWLTEKAQLQAKLEDIRSIL
                     SELQSTLEAKRSEMEAIGDQRGRLLEQLAADKEQRSALVSEYNVLSDMEARRQGLNRT
                     LIELLNSESGKRPYIEGIVADVINAEPMYAPAVEAALEGLTDALLVNSTGDFLNDHSL
                     QEKLDGRIRVICMDRIPPFCDASDLSQEQGVLGRMVEFVRYEGRFGPLVWNLLGHIVL
                     VESLDAAVRLWREFGGRFRFVTPAGQVFSGGCMLNIGPLGKKTGLISRKSRLVQLQAE
                     LDELSCRIRDKEAALEEQEHKNEHLSGLCQELRTSIYEANTERVDAEARLRVLDDNIC
                     RVKQEQPVITGEIEQLEEEIQQSVQREHLSRQKLEELEQINQQRSEHIRLLTSRVEQQ
                     RQLQRQHDAELTNLKVQAGQIQEQRKAVSQQIASLQSQLHHTRMAIESARTEALACEE
                     QTVQTQRTLLAAESRLSDLYLEKEQAGRHSIELHRQHQQLSAQQEQNQEELRVHQARQ
                     SEIEQQIHQLELELSQLAVKEEDLTLRVREELQIELADAYENYRQENIDWEQVRQQIA
                     DLRSKIERLGNVNVDAIDQQKDLEERSQFLTAQVEDLHQSKHQLEDLIERINKESREK
                     FALTFEQIRQNFQQLFRKLFGGGKADILLETPEDILESGIEIIARPPGKETRTISLLS
                     GGEKTMTAIALLFAVFQSKPSPFCVLDEVDAALDEANNERFNLIVQEFKKQSQFIIIT
                     HSKRTMSIADILYGITMQTQGVSKKISVTFEEAQRDTKAAVA"
     gene            6077..7303
                     /locus_tag="PLE88_08435"
     CDS             6077..7303
                     /locus_tag="PLE88_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOM76526.1"
                     /translation="MNKLDIYRLKNGMTLLGERIEHVHSVSFHFLLPAGAALLPDGCC
                     GAGEVIADWLFRGAGDRSSRQLIDALDGLGIHHSTSVSAYHLALRASMEAGNLNKALP
                     LFADIILRPQLAPDQFEPARQLALAELAGLDDDPRQKTLIVLYEQFFPDPLGRPSVGK
                     HQELAWLTAEKTAAVVRSMFDPARVIFGICGKYDFDAVCNQMEKLFDSCTGKGLGESS
                     LKQRGKPYTHLQTDGAQVHIGLMTPAEPATSRFFYEIAAAVSVLSGSMSSRLFTEVRE
                     KRGLCYAVGANYRTLRDAAGISCYAGTTPEKAQETTDVILEQFRRLREGISEDELLRA
                     KVGLKTSLIMQTESTSARAAGITSDYFLLGRIRPIEEIRQKIEALSAENIVGYLQSHP
                     FEHFTAVSIGPRSISV"
     gene            7319..8542
                     /locus_tag="PLE88_08440"
     CDS             7319..8542
                     /locus_tag="PLE88_08440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOM76527.1"
                     /translation="MDFKHRQLSNGLTLIGEVNPAARSAAVGFFVRTGARDETAPLSG
                     VSHFLEHMLFKGTASLSALEVNEAFDRLGAKFNAFTSEENTVYYAAVLPEYLIPAAEL
                     WMELMRPALRDEDFAVEKNVILEEIAMYKDLPQFEVMDQGRAQHFGEHPCGNSVLGTQ
                     ESISALTADQMRSYFARRYVPNNMVLACCGNFDFDALCKLAEQKCSCWNASETYRWLS
                     DFRGTFEKKCRPNPNLARQHICLLSPSVSMQDPRRYAASLLGMILGDDSGSRYYWALT
                     EPALAETAVMQCESMDGTGVFYSYICCDPNNTQQVLNCIQDIYASVYTQKISETELNA
                     AKNKVLSALALKCEQPMGRLVSLGFNWIYNSRYLSVRQDIESVKAVTIRDIYQLIEEY
                     NPGHYTLFSIGPKEV"
     gene            8632..11091
                     /locus_tag="PLE88_08445"
     CDS             8632..11091
                     /locus_tag="PLE88_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76528.1"
                     /translation="MRNEKNDISLDADILQSKADIQAALRAAGKSVLNETPAVQGEAS
                     ISSQDTLLGIDLSSLEDQQEDSSASKDPQIPDMSAPASTEMASPSDELIQTLEDLDRE
                     NKQLKEQMQQLHEQNAILKGENLKIKDAEELYKNEIRQLRSELSAVEIALSQEQSKNR
                     SLTAKISELEGLNTAHEEQHKNLSEQLEQIRQEKLALAEEMDRLRAEHSLYQERTGRE
                     IAELNLHNEQLASERMALKKEYDLLHQDYQASQMQADRDSDKLMEQIEELQQANDNLR
                     QSLSRLQKEASAADIVSQELEEKTQKLEALQIQLAQTEQQLNSERQTASALRAEIKRL
                     EVQIHEDAGCRQTLQEELTSLKSQFEQERLSLVQQYEILSKQHQKLERDYGQMRHEYE
                     AYQAQAETELSQMTEQMAEVRKEQQRQEETIRRLNAELLAAGKDSDKLAEANTKCDML
                     QSELADARRQLEEERQSKDQLHQQVQHYRTAAQEAAHFNMAIQKELAELKEHNEHQQQ
                     MITGQLEHALLEKQRIQNGFELLRKEYSEYKKQSCAETDNLKKQIEHIKADYQSLKHN
                     YENILPENNDLHKRIQEYQRRQDELIAELAAVHQEFSNAKITFTTQIEALQKQQQQAI
                     LQNEQLQETVSSLQEQLTQAQKNANVYRRKADLFDTVQAFWQQTINEEEPSAVSAPND
                     KIEMLPQHLPFENPEPEQAKPVSQETSAAFDEAAPIPAFNLAEQIMAEHRRSISARRQ
                     RIEPSQSVPRNEPIRHVVEHFVAHPSYAAQNQSDEFSAAFWTDGSLSAFQQELLKEIV
                     LRDIESRPIRQNTPKAFTMTS"
     gene            complement(11174..11368)
                     /locus_tag="PLE88_08450"
     CDS             complement(11174..11368)
                     /locus_tag="PLE88_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76529.1"
                     /translation="MMNSGQKNTGSWAADGLDGVQKCRYIGGAGRGFEGHSRRLIYCC
                     FPLPETASARPPYAYTRRSH"
     gene            11411..11794
                     /locus_tag="PLE88_08455"
     CDS             11411..11794
                     /locus_tag="PLE88_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76530.1"
                     /translation="MAQKGPCLLIDLDTRRDAVARAFDIHSAEFSPHLKLSPIQTDID
                     NLWIWPAQYFDLLRQMNLRSLLEYAGTKYQTILLYAPTLTTLPDRRQIAACSQYAIVF
                     KGGHSNHSILNSLLQACRCRILCEA"
     gene            complement(11798..12949)
                     /gene="pgl"
                     /locus_tag="PLE88_08460"
     CDS             complement(11798..12949)
                     /gene="pgl"
                     /locus_tag="PLE88_08460"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOM76531.1"
                     /translation="MRYLAIDHGQKRTGLAVCDASETVITPHSVIDVSADSRLIEQIS
                     KVLKDEKIEAIVIGLPVNMDSTEGPRASAVRQFARRLAEQTACPIYFQDERLSSFEAK
                     SLATEFDWTRQQKKKRLDALAAADILRSFLSSPKACLAKLHLIKAANLEMLSELALDF
                     FCKAASEAIRQGRNFYAALSGGQTPLRFFDKLPDAALCWKRIHLFWVDERCVDPDNPA
                     SNFGQARRAFLQKIDIPSENIHRIRGEHQDSTAAAAEYEENIRSAFHLAPDQWPQFDL
                     IVLGVGSDGHIGSVFPDAYHLLETNALASVVQRDDYRRITLTMPVIQNARRILVLAAG
                     RSKADILRCIFTSEAQPLRYPIHGLWPVLHKVTWIMDEAAASLLEAARA"
     gene            complement(12976..14061)
                     /locus_tag="PLE88_08465"
     CDS             complement(12976..14061)
                     /locus_tag="PLE88_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOM76532.1"
                     /translation="MDYAVILAGGTGKRLWPLSRQRRPKQILKLLDGQTLLRKCFERL
                     KAVFDFRNILVLANADYADLIRENLNELPEENVIAEPAVRDTAAAIGLASAVLQKYDS
                     SAVMAVVTADQILEPAEAFESAMRAALAFVRENPQGLITFGIKPTFPSTQLGYIYFGD
                     EVCRQGGKAVYRINAFKEKPDLHTAQEYLESGQYRWNSGLFVWRCGTILQQLAQHLPA
                     CIEPLRHIQADWGTAKQQQTLAEWFPKMPKISIDYAVMEKSDQVYGISLDCRWLDMGS
                     FTALADVIESDENDNIIVAEHSELMDCRNMIIITEDKDHLIAAIGLENMVIAHSKDAT
                     LVCPISQNERIKELLENVRKHKDGKYL"
     gene            complement(14089..15498)
                     /locus_tag="PLE88_08470"
     CDS             complement(14089..15498)
                     /locus_tag="PLE88_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOM76533.1"
                     /translation="MSQTKLSLYYKNVTADVIGPEHGITKQQFDELAQKTRPAIAECN
                     RQRKNGAIPYRDLPYHKQYPSQVKHVAADIKPGCENFVVLGIGGSALGNIALQTALNP
                     YMYNLDEKQRKGPRLFVFDNVDPAQFGSFLDWIADKLDKTVFNVISKSGRTAETAAQF
                     LIIRQILIEKLGTDGYRRQVVATTDARSGTMRKIADEDKLRTLIVPDGVGGRFSVLSP
                     VGLLSAAVCGIDIDALLEGARAMDEKVSREDFFANPAAVNAAVNWHYYHRGKRISVMM
                     PYAYGLKDLSDWYRQLWAESLGKSCDLKGSKVFVGPTPVKALGTTDQHSQVQLYREGP
                     NDKLFTFLQVSEFQPDIVIGPQPAAAPELAYLAGQKMSKLLNSEKLATEYALLYDKRP
                     CMTILFDRIDADAIGQFIYLYEVTTSIAGLLFGIDAYNQPAVELGKEATFALMGQAEY
                     ADLAEKIKPVAQVDTAYLV"
     gene            complement(15534..>16525)
                     /locus_tag="PLE88_08475"
     CDS             complement(15534..>16525)
                     /locus_tag="PLE88_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOM76534.1"
                     /translation="EIRDLLSYLKLIVNPADDVAFNRAIATHPRGIGKTSIENLGIYA
                     RRHGLSLLEAAEKADQIDTISRPTRMRLIEFAAMIRRFQQEASGGIAPLMDCVFVESG
                     LADALKSDPDAVENINGLVNAAAEYEQQTEQPSLTDYLQMIALYSDVDAYNADSGKVS
                     LMTLHAAKGLEFDCVFIIGLEEGILPHERSSWGGIDDLEEERRLFFVGITRARIKLII
                     TYARHRVMRGQFVRNTPSQFLYEIGFKGESLDAGWETAGQEDELIACPAAEKKRSAYA
                     FEVNELVEHAKFGIGRVKEFLDLGQDSIVVVKFNSGNTKSLMVKYAKLQRIGR"
BASE COUNT         4378 a         4271 c         4012 g         3864 t
ORIGIN      
        1 tcgtcagctc aatagcagaa atccttgccg atatgaacat tgctgatgcc aaggtaacca
       61 gcgctggggg tctggatact ttaatcaaac aattcaaagc tgcccgccgc aatattgcac
      121 agggtcttgc ctccatcgaa cgacttgaag agcaaacggc cagactcgat gaacagttct
      181 ttgaaattga caaacagatg cgcagcggct tgatgacccg tgaggtaact caaggcggca
      241 gcagcgttta tcagcgtcgc agcagaaacg gtctgccttc tgattacaac cgctctctca
      301 gggattctgc cggggacagt ttgccgtcca atcagacttc cgcgtccggc cgaatccgca
      361 gtccccgctc ggagagcata actacaatcg aacctgcggc caatattgaa gtcgtaagaa
      421 gccagcttat tgcttcgctt cggaatctta ccgcagagta ccgccgggtt attaatcttg
      481 ccaaacgctg gccggggggg cttccagccc aagtatccat cgaccagctc cagactaata
      541 tgaactcggc agcagctcaa ctggatagtc tataccgcta tcagccgatt tatcaaagtc
      601 aacccattca gccatcggca ggcaggctta tccgctgatt ttttcgctat gacaatttta
      661 ccgccgtaaa ccatatattg tggtttacgg cttttttatt acaagcagta gaatattctt
      721 gacgatttaa tgtcttttta atatcgtatg ctttgggttt atacaaaaca gaactattta
      781 taaatgcggc aattggattg aaaggaaagc aataatgatt cattcatcca ttattaaata
      841 cctttgggtt ttgccgctgc ttttcggcct tatcggctgc actcagccgg caccgactac
      901 aaatcccgct gcggctgtgg atcctcgaag tacgataggt tcaatttccg cctattatgc
      961 caacgaatct gtggcggtcc ggggaatcgg tattgttgcg ggccttgccg gaactggttc
     1021 ttccgaatgt cctcccggca tccgtgagga actggaaaaa tatatttgga aacaaatgcc
     1081 caatatagcc gttaatcccc gcttatttat agaaagtcag aatacagctg tcgtagaaat
     1141 catcggcgtt attccgcctt atgcgacacg tcagacggct tttgatgtta ttgtgcgccc
     1201 tctatccagc acccaaacaa cctcgcttaa cggcggctac ctttatactg ctgaattaaa
     1261 ggagttaagc cgtctgacaa atgtcggaca atttgcacag tattccaaaa ctttggcagc
     1321 ggctgaaggg ccaattttta ctaaactgtc ccaagatgcg tcagacagaa aatggtatgt
     1381 cctcggcggc ggccgtcctg ctgaagacgg ttttgtcaaa ttaatactca aaacccccga
     1441 ttttgtagcc gctaatgcga ttcgcaatcg aatcaacgaa cgtttcgggc ccaagactgc
     1501 cgttcccgtt tcagcaggag aatgcaatct gttctttccg gccaaatatc ttgaccaaaa
     1561 agagcggttt ttaaaaatgg tacaaaccct catcctcgca gacaatcctg atatccgaaa
     1621 tgactatatg cagactcttc ttgaccggct tgtcagggat tctgataaag aatctgcaga
     1681 aatcgccctt gaaggcatcg ggaaaccagt acttgacagt ttggagtctt tgctggagca
     1741 taatgatcct gctgtgcagt tttatgccgc ccgatgcatg ttaaatatcg gcgacagccg
     1801 ccccctgcct gtcttgagaa agatcatcct cgagccggca agcccctacc ggatagccgc
     1861 tattcgtgcc gtcgggcaaa gtgccaaacg cagcgatgcc cggcctattc tcttgaatgc
     1921 cttacatgat aatgatattc aggttcgctt ggcggcgtat gaaatgctgg gaccaatgga
     1981 cagccccgaa atttcccgca ccttagttgc caagggatct tttgccatcg accgtgttct
     2041 atgctccggc ccgaaaatga tttacgttta ccagcaattg tcaccccgca ttgttctgtt
     2101 tggttcgccg ctgcattgtg aaaaagatat atttatccag tcggatgatg agatggtaac
     2161 cattaatgcc cgctcgggcg acaaatatat cactgtttcc cgcaaacatc ccagccggcc
     2221 gagagtcgtt gggccgttgt cttgttcgta tgaattaagc agtttaattc agaccttggg
     2281 ggaggttccg gatgtcaagg gtacatctgc accccgcccg ggattaaata ttccatatgc
     2341 agatattatc cgcattttac agaaaatgtg ccgacaggat gccattaaag cgcaatttat
     2401 agctggccca gagccggaaa agaatcctct tttgcagaat ttgccggtaa taggccgata
     2461 aaataagaag catttttcta cctgtaagcc aaaggattat atgaaacttg aaaaaattat
     2521 tataaacgga tttaagagct ttgccgacaa aactgaattt tcaatcagcc atcccatcac
     2581 tgccatcgta ggccccaacg gctgcgggaa gagcaatgtc gtggatgcac tgaagtgggt
     2641 actgggcaac cagagtccta aaagtctccg cagcggccag atgtcggacg tcatcttcag
     2701 cggttccagc agccgcaagc ccagcggaat ggctgaagta acgctgcatt tttccgacgt
     2761 tcacggtctt gaactggaac aggatgagct tcagatttct cgtcgtcttt tccgcagcgg
     2821 tgaaagcgat tatttgatta acgggaaatc ctgccgtcta aaagatatcc gtgaactgtt
     2881 tatggacacc ggcatcggcg tcagtgcgta ttcgattatt gaacaaggtc agattgacca
     2941 gcttctcaag tcctcaacgc aggaccggcg gataattttt gaagaagcag ccggcatcag
     3001 caagtacaaa gcccacaaaa aagaggcctt gcgcaagctg gagcgtaccg agcagaatct
     3061 gctgcgtctg gctgatattg tcaacgaagt ccaaaagcag cttcgcagca ttaagctcca
     3121 ggccggcaag gcccgcagtt atttagaata cagcgaaaag ctcaaggagc tgcgcgtcaa
     3181 ttattcactg gcagaatatg accgcattgt tacccagcag cgggagaaaa atacggtact
     3241 ggcggactgg aaggcgcaat ttggccaaac tgccgctgat ttagcacggt gcgatgcagc
     3301 tgtcagccag ctaacacacg ccatcatgga aacggactcg caaatcaacc gttgggacgg
     3361 cattttaatt tctgcacgca gccggattga gcagcatcat gaccgcattg gctttctcaa
     3421 taaccgtctt cacgagctgc aggacagaaa gaaaaatgct gctgatcaga ttgccaaact
     3481 catggagcag acttgtcagc ttgcctcgca gataaaagcc tgccagaaaa ttcttcagga
     3541 agtcgagcaa ttatcggaaa caactgccag ccggctgcat caggccgaac aggttatcag
     3601 caccattacc gcccaatgtg cagaactgga agcccagctg gacgatgaaa aatccggaat
     3661 catcgacatc gtccgccgga cagcccagct gcataacgaa atagaaagtt taaccagtta
     3721 ccgcagcact ctttccggcc gaaagacgcg gctttcagga aaagcggaag aaacacagca
     3781 ccagctttcc caatggctta cagaaaaggc gcagcttcag gccaaactgg aggacatacg
     3841 ctcgattctt tccgagctgc agagtacctt agaagcaaaa cgcagcgaaa tggaagccat
     3901 tggagaccag cggggacgcc tgctggagca gctggcggcg gataaagagc agcgaagtgc
     3961 gctggtcagc gaatacaatg tgctcagcga catggaagcg cggcgtcagg gattgaaccg
     4021 cacgctgatt gagctgctca attccgaatc tggcaaacgg ccctatattg aggggattgt
     4081 ggcggatgta ataaatgccg aaccaatgta tgcaccggct gtagaagctg ccctcgaagg
     4141 ccttacggat gccctgctgg tcaacagtac cggggacttt ctaaatgacc attcgctgca
     4201 ggagaaactg gacggccgca tacgggttat ctgcatggac cgcattccgc cgttttgtga
     4261 tgcatccgat ttatcgcagg aacaaggtgt ccttgggcga atggttgaat ttgtgcgcta
     4321 tgaggggcgt tttggaccgc tggtctggaa tcttctgggg catattgttc ttgttgagtc
     4381 tctcgatgcg gctgtgcgtc tgtggcggga gttcgggggg cgctttcgtt ttgtaactcc
     4441 ggccggacag gtattttccg gcggctgcat gctgaacatc ggaccgctgg gcaaaaagac
     4501 aggcttaatt tcgcgcaaaa gccgtcttgt ccagcttcag gccgaattgg atgaactcag
     4561 ctgccgtatc cgcgataaag aggccgccct tgaagaacaa gagcataaaa atgagcattt
     4621 gtccggcctt tgccaagaac ttcgcacaag catttatgaa gccaacaccg aacgggtgga
     4681 tgcagaggca cgcctgcggg tattggacga caatatctgc cgggtcaaac aggaacagcc
     4741 ggttattact ggtgaaattg agcagctgga agaagagata cagcagtcgg tgcagagaga
     4801 gcatctgtcc cggcagaaac tggaggaact tgagcaaatc aatcagcagc gaagcgagca
     4861 tatccgtctg ctgaccagcc gcgttgaaca gcagcggcag ctgcagcggc agcacgatgc
     4921 ggaactaacc aaccttaaag tgcaggccgg acagattcaa gagcagcgca aggctgtcag
     4981 ccagcagatc gcctcgctgc aaagccagct acaccacacg agaatggcca ttgaatcagc
     5041 ccgcaccgaa gcgctggcct gtgaagagca gaccgtccaa acacagcgga ctctgttggc
     5101 agccgaatcc cgtctgtccg atttgtatct tgaaaaagag caggccggcc ggcacagcat
     5161 cgaactgcac cggcagcatc agcagctgtc tgcacagcag gagcagaatc aggaagaact
     5221 gcgtgtgcat caagcccgac agagcgaaat tgaacagcag atacatcagc tcgaactgga
     5281 attgagccag ctggctgtca aagaagagga tttgaccctg cgcgtccggg aagagctgca
     5341 gatcgaactg gccgatgctt atgaaaacta ccggcaggag aatatcgact gggaacaggt
     5401 gcgtcagcag atcgccgatt tgcggagcaa aattgagcgg ctgggcaatg tcaatgtaga
     5461 tgcgatagac cagcagaagg accttgagga acgcagtcag tttttaaccg cgcaggttga
     5521 agacctccat caaagcaaac atcagctcga agatctgatt gagcggatca acaaagaaag
     5581 ccgtgaaaaa tttgctctca cattcgagca aatccgtcaa aactttcagc agcttttccg
     5641 caagctgttc gggggcggca aagcagatat tctgctggaa accccggagg atattctgga
     5701 aagcggcatc gaaatcattg cccgtccgcc cggcaaggaa acccgtacga tcagtctgct
     5761 cagcgggggt gaaaaaacca tgactgcaat tgcgttgctg tttgctgttt tccagagcaa
     5821 gccgtccccc ttctgtgtgc tggatgaagt cgatgccgca ttggatgagg ccaacaatga
     5881 acggttcaac ctgattgtgc aggagttcaa aaaacagtcc cagtttatta tcatcacgca
     5941 ctccaagcgc accatgagca ttgcagacat tctgtacgga atcacaatgc agacacaggg
     6001 cgtcagcaaa aaaatcagcg taacgtttga ggaagcgcag agagatacca aagcagctgt
     6061 ggcatagggc aacacgatga ataagctgga tatctatcgg ctcaaaaacg gaatgacgct
     6121 tcttggagaa cggatagagc atgtgcattc ggtttcgttt cactttctgc tgccggcggg
     6181 ggcagcacta cttccggacg gatgctgcgg ggctggcgag gtaatcgcgg actggctgtt
     6241 tcgcggtgcc ggcgatcgct ccagccgcca gctgattgat gccttagacg gattgggcat
     6301 tcaccacagt acatcggtat cagcgtacca tctggcgctg agggcctcaa tggaagccgg
     6361 caatctgaat aaagccctgc cgttgtttgc ggatatcatt ctgcggcccc aattagcccc
     6421 tgaccaattc gagccggcgc gtcagcttgc gctggcggag ctggccggtc ttgatgatga
     6481 tccccgccaa aaaacgctta ttgtgcttta cgagcagttc tttcccgatc ccttaggccg
     6541 tccgtctgta ggcaagcatc aagagcttgc ttggctgaca gcggaaaaaa ctgcagctgt
     6601 agtccggtct atgtttgatc cggcgcgcgt tatattcggg atttgcggca aatatgattt
     6661 tgatgcggtt tgcaatcaaa tggaaaaact gttcgattcc tgcaccggca agggtcttgg
     6721 agaatcatct ttgaaacaac gcggaaagcc gtacacccat ctgcaaactg acggggcgca
     6781 ggttcatatc ggactgatga cgccggcaga acctgcaaca agccgtttct tttatgagat
     6841 agcggcagct gtttcggttt tatccggcag tatgagcagc cgtctgttta ccgaagtccg
     6901 cgaaaagcga ggcctttgct atgccgtagg ggctaattac agaacgcttc gcgatgcagc
     6961 cggcatctcc tgttacgccg gaacgacacc ggagaaggcg caggaaacca cagatgttat
     7021 tttggagcag ttccgccggc ttcgggaagg catatcagag gatgagctgc ttcgggccaa
     7081 agtcgggctg aagacctctc tgattatgca gaccgaatcg accagcgccc gtgctgcggg
     7141 aatcacttct gattattttc tgctgggccg cattcgtccg atagaagaaa tacgtcaaaa
     7201 aatcgaggcc ttaagcgcag aaaacattgt ggggtatctg cagtctcacc cctttgagca
     7261 ttttacagct gtcagcatcg gtcctcgttc aatatccgtt taagggaaac cgtcatttat
     7321 ggatttcaag catcgacagt tatccaacgg cttaactctc atcggcgagg tcaatccggc
     7381 agcccgatcg gctgccgttg gatttttcgt ccgcacaggt gcccgcgatg aaacagcgcc
     7441 gctcagcgga gtatctcatt ttctcgagca tatgctgttc aaaggcactg cctctctgtc
     7501 ggctctggaa gtcaacgagg cgtttgaccg tctgggtgca aagtttaacg ccttcaccag
     7561 cgaagaaaat accgtctatt atgcagccgt tctgccggaa tatcttatac ccgcggcaga
     7621 attatggatg gaattgatga ggccggctct tcgggatgag gattttgccg ttgaaaaaaa
     7681 tgtgattctt gaagaaatcg ccatgtataa agatctcccg cagtttgagg tgatggatca
     7741 gggccgcgcc cagcatttcg gcgaacatcc ctgcgggaac agtgtacttg gaacccaaga
     7801 gtccatatct gccttgacgg ccgaccagat gcggtcgtat tttgcccggc ggtatgtccc
     7861 aaacaatatg gtccttgcct gctgcggaaa ttttgatttt gatgcactat gcaaattggc
     7921 tgaacagaaa tgcagctgct ggaatgcctc tgaaacatac cgctggctga gtgatttcag
     7981 agggacattt gaaaaaaaat gtcggcccaa tccgaatctg gcacgtcagc acatttgcct
     8041 gctcagtccc tcggtctcaa tgcaggatcc gcgccggtat gcggcttccc tgctgggaat
     8101 gattttgggt gacgacagcg gctcccgtta ttactgggct ttaacagagc cggcccttgc
     8161 agaaacagcc gtaatgcagt gtgaatcaat ggacggcaca ggagtgtttt acagttatat
     8221 ctgctgtgat cccaacaata cacagcaggt actgaattgc attcaagaca tatacgcatc
     8281 cgtctataca caaaaaatct ctgaaacgga gttaaacgcc gctaaaaaca aggttctgag
     8341 tgccttagcc cttaaatgcg agcagccgat gggacggctg gtttccttag gattcaactg
     8401 gatttacaac agccgctatt tgagcgtccg gcaggatatc gaatccgtta aagcagttac
     8461 catccgcgat atttatcaat tgatcgagga gtataatccc ggtcactata cgctgttctc
     8521 cattgggcct aaagaggtat aattatctct ccaatttata aaaatcccca atctcttgat
     8581 ttttaaaggt gattatgtta aaaggggtaa aatgttagat tggatggctt gatgcgcaac
     8641 gaaaaaaacg acatctcgtt agatgcggat atcctgcaga gcaaagcgga cattcaagcg
     8701 gctctccgtg cagccggcaa gtccgttttg aacgagacgc cagccgtcca aggtgaagct
     8761 tctatatctt cccaagacac tttgttgggc attgatttaa gcagtttaga ggaccagcaa
     8821 gaggattctt ctgcttccaa agatccacaa ataccagaca tgtccgctcc agcatctact
     8881 gaaatggctt ccccttcgga tgagctcata caaacccttg aggacctgga cagagagaat
     8941 aagcagctga aggagcagat gcagcagctt cacgaacaaa atgccatttt gaaaggcgaa
     9001 aatttaaaga taaaagatgc agaagaactt tacaaaaatg aaatacggca gctgcgaagt
     9061 gaactcagcg cagttgaaat cgcactttcg caggaacaga gcaagaatcg ctctcttaca
     9121 gctaaaataa gcgagttgga gggtctaaac accgctcacg aggagcagca taagaatctg
     9181 tcggaacagc ttgaacaaat ccgtcaggaa aaattggcac tggctgaaga aatggaccgt
     9241 cttcgcgcag agcattctct ttatcaggaa cggactgggc gtgaaatcgc tgaattaaat
     9301 cttcacaacg aacagctggc cagtgaacga atggcactga aaaaggaata cgatctgctc
     9361 catcaggatt atcaggccag tcaaatgcag gccgaccgtg acagcgacaa actaatggag
     9421 cagattgagg aacttcaaca ggcaaatgat aacttacgac aaagtctttc gcggctccag
     9481 aaagaagctt ctgcagcgga catcgtttca caggaactgg aggaaaaaac ccagaagctg
     9541 gaagccctcc aaattcagct ggcacagacc gagcagcagc ttaattctga gcgccagaca
     9601 gcttctgcct tgcgggcgga aatcaagcgt cttgaggtcc aaatccatga ggatgccgga
     9661 tgccggcaaa cactgcagga agaattaaca tctctgaaaa gccaattcga acaggaacgc
     9721 ctcagtcttg tgcagcaata tgaaatcctc agcaagcagc atcagaagct tgagcgcgac
     9781 tatggtcaga tgcgtcatga atacgaggcc tatcaggcac aggccgagac agaattatct
     9841 caaatgaccg aacagatggc tgaagttcgc aaggagcaac agcgtcaaga agaaacaatc
     9901 cggcggctta atgctgaatt gcttgccgca ggcaaagatt cagacaagct tgctgaagcg
     9961 aacaccaaat gcgacatgct tcaatcggaa ttggcggatg cccgccgtca gctggaggag
    10021 gaacgacaat ccaaagacca gctgcaccag caggtccaac actaccggac cgctgcccaa
    10081 gaggcggctc atttcaacat ggcaattcaa aaggagcttg cggaattgaa ggagcacaac
    10141 gaacatcagc agcagatgat aaccggacag ctcgagcatg ccctgctgga aaaacagcgg
    10201 attcaaaatg gcttcgaact gcttcggaaa gaatattcgg aatataaaaa gcagagctgt
    10261 gcggaaacag acaacttaaa gaagcagatt gaacatatca aagctgatta ccagagcttg
    10321 aagcataact atgagaacat cctgccggaa aataatgacc tgcacaaacg cattcaagaa
    10381 taccagcgcc gtcaggatga gcttatcgcg gaattggccg ctgttcatca agagttcagc
    10441 aatgccaaaa tcacattcac aacccagata gaagctcttc aaaaacagca gcagcaagcc
    10501 atcctgcaga atgagcagct tcaagaaaca gtatcaagtc tgcaggagca gctaacgcaa
    10561 gcacagaaaa atgcaaacgt ttaccgccgc aaagcagacc tttttgatac cgtccaagcc
    10621 ttctggcagc aaaccataaa cgaagaggaa ccatctgccg tatctgcgcc aaacgataaa
    10681 atagaaatgt tacctcagca cttgcccttt gaaaatcctg agcctgaaca agctaagccg
    10741 gtttcacagg aaacatctgc agcatttgat gaggcagcac caatcccggc attcaatctg
    10801 gctgaacaga ttatggcgga gcatcgccgt tctatttccg cacgccgtca gcggattgaa
    10861 ccatctcaaa gtgtcccgcg aaatgagccc attcgtcatg tagttgagca ttttgtagcc
    10921 catccgtcct atgcggcaca aaaccaatcc gatgagtttt cagccgcttt ttggacagat
    10981 ggatcgctca gtgcctttca acaggaactg ttgaaagaaa tagtcctgcg ggatatcgag
    11041 tccagaccta tcagacaaaa cacgccaaag gcattcacga tgacctcctg aaacagcaat
    11101 gaacatccat gttgcgtatt ttcttccagg atggataaca atttcgggca ccacagatgt
    11161 cctattggct gttttaatgg ctgcggcggg tataggcata tggtggtctt gccgatgccg
    11221 tctccggcag cggaaaacag cagtaaatca gccggcggct gtgcccctca aatccccttc
    11281 cagccccccc gatatagcgg cacttttgaa ctccatccag tccatcagcc gcccacgacc
    11341 ctgtattctt ctggccgctg ttcatcatcg cgatttgcca gtcacgatac ctatacgctt
    11401 agcgattcaa ttggctcaaa aaggaccctg cctgctgatt gatctggaca cacggcgaga
    11461 tgctgttgca cgtgcattcg acatccattc ggcagaattt tctcctcatc tgaaactgtc
    11521 cccgatacaa acagatattg acaatctgtg gatttggcct gcacagtatt ttgatttgct
    11581 gcggcaaatg aatctgcggt cattattaga atacgccggc accaaatatc aaacaatcct
    11641 gctctatgcc cctacgctga ccacccttcc tgaccgcagg cagatagctg cctgctcgca
    11701 atatgccatt gtattcaagg gggggcactc caaccattcg atattgaact ccctgctgca
    11761 agcctgccgg tgccggattc tctgcgaggc ctgacagcta tgctcttgcg gcttccagca
    11821 aggaagcagc cgcctcatcc ataatccatg taactttgtg cagaaccggc cacaaaccgt
    11881 gtattggata gcgcaaaggc tgtgcttcag aagtaaaaat gcagcgaaga atgtctgctt
    11941 tgctgcggcc ggcagccagc accaaaatac ggcgggcatt ttgaataacc ggcattgtca
    12001 gtgtaatccg ccgataatca tcccgctgta ctaccgatgc cagagcattt gtctccaata
    12061 aatgataggc atccggaaaa acagatccaa tgtgcccatc gctgccgaca cccagtacaa
    12121 tcaaatcaaa ctggggccac tggtcagggg caagatgaaa tgccgaacgg atattctctt
    12181 catattctgc agccgcagct gtgctgtcct gatgttcacc ccgaattcga tgaatattct
    12241 cagatgggat atcaatcttc tgcagaaatg ctctgcgggc ttgtccgaaa ttgctggctg
    12301 gattgtccgg gtctacacac cgctcatcca cccagaacaa atgaatacgc ttccagcaca
    12361 gtgcagcatc aggaagttta tcaaaaaagc gaagcggggt ctgtcctccg gacaaggctg
    12421 cgtaaaaatt tctgccctgc cttatcgctt cagatgccgc cttgcagaaa aaatctaatg
    12481 ccaattccga cagcatttcc aaattagccg ctttgataag atgcagttta gccaagcagg
    12541 ccttggggga actgagaaag gaccgcagga tatctgcagc agcaagtgcg tccagacgct
    12601 ttttcttttg ctggcgcgtc caatcaaact cggtagccag cgacttggct tcaaaactgc
    12661 tgagccgctc atcctgaaaa taaatcggac atgctgtctg ttcagccagc cggcgtgcaa
    12721 actggcgaac agcagaggca cgaggccctt ctgtgctgtc catattaacc ggcagcccta
    12781 taacaattgc ttcaattttc tcgtctttga gaactttgct gatttgctca atcaatcggc
    12841 tgtcggcaga aacgtcaata actgaatgcg gtgttattac tgtttcggag gcgtcacaga
    12901 cagccagccc tgtccgcttt tgaccatggt caattgcaag ataccgcata agtcgatgct
    12961 cccgcagttg tgcgttcaga gatatttgcc gtctttgtgc ttccgaacgt tttccaaaag
    13021 ttctttgatg cgctcgtttt gactgattgg acagacaagc gtggcgtctt tgctgtgggc
    13081 aataaccata ttttcaagcc cgattgccgc aatgagatga tctttatctt cggtgattat
    13141 aatcatattc cggcagtcca tcagctcgct gtgttcggcc acaataatat tgtcattctc
    13201 atccgattca attacatcag ccagagctgt aaaagagccc atatccagcc agcggcagtc
    13261 cagagaaatg ccgtacacct gatcagactt ttccataacg gcatagtcga tgctgatttt
    13321 gggcattttt ggaaaccact ctgccagcgt ctgctgctgt ttggcagttc cccaatccgc
    13381 ctgaatatgg cgcaagggtt cgatgcaggc aggcagatgc tgggccaact gctgcagaat
    13441 ggtaccgcac cgccagacaa acaatcctga gttccagcgg tattggccgc tttccagata
    13501 ctcttgggca gtgtgcagat cgggtttttc cttgaaggca ttaatcctgt aaacagcttt
    13561 gcccccctgc ctgcatacct catccccgaa ataaatgtag cccaattggg tactcggaaa
    13621 cgtcggctta ataccgaatg ttatcaggcc ctgcggattt tcgcgaacga aggcaagggc
    13681 agcacgcatc gccgattcaa aggcctctgc cggttcgaga atctggtcgg ccgtgaccac
    13741 tgccatgacg gcgctgctgt cgtacttctg cagcacggct gacgccaatc cgatggctgc
    13801 cgctgtatcc cgaaccgccg gctctgcaat aacattctct tccggcagct cattgagatt
    13861 ttcgcgaata aggtcggcat aatcggcatt ggccaaaacc agaatattcc gaaaatcaaa
    13921 aaccgctttc agccgctcaa agcacttgcg cagcagcgtt tgaccgtcca gcagcttcaa
    13981 tatctgcttg ggccgcctct gccggctcag cggccaaagc cgcttgcctg ttccgccagc
    14041 caagattacc gcataatcca tcctgtccca ctcccttcag ttgaattgtt ataccagata
    14101 cgctgtatcc acctgtgcta caggtttgat tttttctgca agatcagcat attcagcctg
    14161 tcccatcagg gcaaaagtcg cctctttgcc taactccact gccggctgat tataggcatc
    14221 aatgccgaac agcagacccg caatcgaggt tgttacctca tacaagtaaa taaactgccc
    14281 aatcgcatcc gcatcaatgc ggtcaaaaag aatagtcatg cagggccgct tgtcataaag
    14341 caaggcatat tctgtagcca gcttttcact gttgagcagt tttgacattt tctgaccggc
    14401 cagataagcc agttcaggtg cagctgccgg ctggggtccg attacaatgt ccggctgaaa
    14461 ttcgctaacc tgcagaaaag taaacagctt atcattcggt ccttcgcgat agagctggac
    14521 ctgactgtgc tggtcggttg tgcccaatgc tttgactggt gtggggccga caaacacctt
    14581 gctgcctttc aaatcacagg acttgcccag actttcagcc cataattgtc ggtaccaatc
    14641 ggataaatcc ttaaggccat aagcataagg catcatcaca gaaatccgct tgccgcgatg
    14701 ataataatgc cagtttacag ccgcattaac cgccgccgga ttggcaaaaa agtcttctcg
    14761 actgaccttt tcatccatcg cccgtgcgcc ttccagcagc gcatcaatat caatgccgca
    14821 gactgccgca ctcagaagac caaccggact gagcacgctg aaccgcccgc ctacaccatc
    14881 cggtacaatc agcgtacgga gcttatcctc atctgcgatt ttccgcattg tgccggaacg
    14941 cgcatctgta gttgccacaa cctgacggcg atacccatcc gtccccagtt tttctatcag
    15001 tatctgacgg ataattagaa actgggcagc ggtttccgct gttcgccccg atttgctgat
    15061 aacattgaaa acggtcttgt ccagcttgtc ggcaatccag tccagaaaac ttccgaattg
    15121 tgccggatct acattgtcaa atacaaacaa acgcggccct tttcgctgct tttcatccag
    15181 attatacata tacggattca gcgctgtctg caaagcgata ttgcccagcg ctgaaccgcc
    15241 aatgccaagt acaacaaaat tttcgcagcc gggttttata tccgctgcaa catgtttgac
    15301 ttgactgggg tattgcttat gatacggcaa atcccgatat ggaattgccc catttttccg
    15361 ctgccggttg cattcggcaa ttgccggccg ggttttctgt gctaattcat caaactgctg
    15421 ctttgttatc ccatgctcgg ggccaataac atctgcagtt acattcttgt aatagaggga
    15481 taattttgtt tgcgacacag ctagcactcc tgattggtca attttcacac tttttaacgt
    15541 cctattcgct gcaacttagc atattttacc atcagcgatt tggtatttcc agaattgaat
    15601 ttcacaacaa caatgctgtc ttggcccaag tctaaaaact ccttgacccg tcctatgccg
    15661 aattttgcat gttccacaag ttcattaacc tcaaaagcat atgcggaacg ctttttctct
    15721 gctgcaggac aagcaatcag ctcgtcctcc tgcccggctg tttcccatcc ggcatccagc
    15781 gattccccct tgaagccaat ctcatacaag aactgggagg gggtattgcg gacaaactgc
    15841 ccgcgcatta ctcgatgccg ggcataggta ataatgagct ttattcgggc acgtgtgatt
    15901 cccacaaaga aaagccggcg ttcttcttct aaatcatcga tccctcccca gctgctgcgt
    15961 tcatggggca gaatgccttc ttccagcccg ataataaaaa cgcagtcaaa ctccaaccct
    16021 ttggccgcat gcagcgtcat cagcgatacc ttgcccgaat cagcattgta cgcatcgaca
    16081 tcactgtaca gggcaatcat ttggaggtaa tcggttagac tgggctgctc tgtctgctgt
    16141 tcatattctg cagccgcatt aaccagacca ttgatattct caaccgcatc cggatcggat
    16201 ttcagcgcat cggccaatcc gctttcgaca aacacacaat ccatcagcgg agcgattccg
    16261 cctgacgctt cctgctgaaa acgccggatc atcgcagcaa attcaatcag ccgcatacgt
    16321 gtcggtcggc tgattgtatc aatctgatca gccttttctg cagcttccag cagtgaaagc
    16381 ccgtgtcgtc tggcgtaaat ccctaaattc tcaatggagg ttttgcctat cccccgcgga
    16441 tgggtagcga tggctcgatt aaaagcgaca tcatctgccg gattgacaat tagcttaaga
    16501 taactaagca aatcccgaat ttcct
//
LOCUS       DAOTBO010000066        15854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3082_length_15854_cov_5.247927, whole genome shotgun sequence.
ACCESSION   DAOTBO010000066 DAOTBO010000000
VERSION     DAOTBO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15854
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_15854_cov_5.247927"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLE88_08480"
     CDS             complement(<1..187)
                     /locus_tag="PLE88_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76535.1"
                     /translation="MDAADEVFDVKRHLKIATRASKLAMAQAESVGRMLQQLLPDCFI
                     TFEAVSTKGDRDPSDFLY"
     gene            complement(320..2791)
                     /locus_tag="PLE88_08485"
     CDS             complement(320..2791)
                     /locus_tag="PLE88_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOM76536.1"
                     /translation="MPKCLMVDPNEKRKATFIQFTPIPVNQYSKTVRDELSRYTKEDL
                     VRIQRDMLILRTFETMLNEIKLRGSYMGIEYMHNGPAHLSIGQEAAAVGQAFHLGIDD
                     HIYGSHRSHGEILAKGLSAIQKLDDAALMQIMKNYFDGQCLKIVEKDAAGSVKDLAID
                     FLIYGTLAEIFGREAGFNKGMGGSMHAFFPPFGIYPNNAIVGGSGDIAVGAALYKKVN
                     KKSGIVIANIGDASSSCGPVWEGLCFATMEQFKTLWDEAHRGGLPLIVNFNNNFYGMG
                     GQPEGETMGFGMLARLGAGLNPQQMHAERIDGYNPLAVADAVLRKRQILEQGDGPVLL
                     DTITYRYSGHSPSDQSSYREKTEVEAWQQHDPIVHFAKELAAAGICEDQAIDAIRQHV
                     EQLIIKACRKAVDKTISPRADLKKANCLLDQTMYSNQKMDSMEPGRKPEVLLSKDQNP
                     RVQALTARSRSAFDETGQRLKDSRCVVLRDALFEAILDRFYADPTLVAYGEENRDWGG
                     AFAVYRGLTEALPYHRLFNAPIAEGAIVGTAVGYALEGGRALVELMYCDFLGRAGDEI
                     FNQLCKWQSMSGGLLKMPVVVRVSVGSKYGAQHSQDWTSLCAHIPGLKVVFPATPYDA
                     KGLMYAALTGTDPVIFFESQRLYGIAEEFVQGGVPLDYYEVPFGQPAVRREGRDLTIL
                     TIGATLYRALEAAKQLEQNWGLTCEVIDARSLVPFNYEPVIESVKKTRKILLASDACQ
                     RGSFLHTMASTIGLLAFDELDAPPVVLGARNWITPPDEVEDAFFPYPSDFLDMIHEHI
                     LPLKGYQVKRPCSAAELIRRSKAAL"
     gene            complement(2814..3611)
                     /locus_tag="PLE88_08490"
     CDS             complement(2814..3611)
                     /locus_tag="PLE88_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOM76537.1"
                     /translation="MTYTIQQRRQAVMNWVYQLGHVSVRRLAEELQVSEATIRRDLQG
                     LAAEGLLELTHGGASVVRNSDYSFISKSMRNVEAKRTIAKMASQMIADGEQIFLDSGT
                     TCFEMTPFLRNKRSLSVIVNSIRTAQELFTPTLKVLMLGGQYRPERMDTVGPLAAETL
                     GRLRGYCAFLGTDGLGMDFGLTSIDIESAHLFGLAARNARQVVLLADHSKFGSPSLYK
                     IADFDCLSTIITDRRPSEQWCAYLEKKNIAVLFPDAGVSQSLLTTGS"
     gene            3769..5376
                     /locus_tag="PLE88_08495"
     CDS             3769..5376
                     /locus_tag="PLE88_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOM76538.1"
                     /translation="MFVIKYPSKGIPASEGVVAAWTASEGKLVKKGEILAEIEISSAV
                     IALESSISGTLRKIMAPDGTPVKVGDALALVGRPEEDISSILSKITPPLPAVDEPKYA
                     KPETSAGVSVESQKDDKRQPNILKKEENMTLAKPIQPAANSGGIIPVLMPQAGQTMEE
                     GTILSWKVKEGDRIAVGQVIMEIETDKAAMEVEAVDAGRIAKIIAPEGTVVPVKTPVA
                     YLAEEGIDIDAFLASGADASVPSVQSADTMAAPAQSRDTESSRQPASVSETGRVKASP
                     AARKMARDKGIDLAGLTAGSGPGGRILSADVQAVQAVPSQVQTYAVDKMRQAIANNLL
                     YSKQNIPHFYAKLTLDAGSLYAVYRKTKEQFKCSINDFITAACARAIRQYPAFRSQYR
                     DGQIVENPAVNIGIAVGTENGLTVPVVLNADRMNLEQLASSSRQVVEAARSGKLKGVG
                     QGVFTITNLGMFGVEEFAAIINPPESAILAVGALREGTAVENGAIRATRLMTITLSVD
                     HRVIDGVLAAEFLKTVKELLEKPEQLI"
     gene            5400..6830
                     /gene="lpdA"
                     /locus_tag="PLE88_08500"
     CDS             5400..6830
                     /gene="lpdA"
                     /locus_tag="PLE88_08500"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOM76539.1"
                     /translation="MTEHYQIAVIGAGPGGYIAALKAARMGAKTAIVEKHHVGGTCLN
                     YGCIPSKALLASAELLHRIRKADSLGIRVNGAVSFDWPSIQKRKDKVLMKLRGGIKGL
                     FAAAGVTLHSGRAVLDGPGRIAVLDEQGSAVSFTADSVILAPGSIPARIPGWPTDTHL
                     VCTSDEALHWKDLPQRLLIVGGGVIGCEFACMMQEYGVEVAIVEMLDSLLPEMETALG
                     AALKDIFIRRGIAVYTGSKVDNLSVKDGSVEAVLSGGQVIKADRVLTAAGRKPAVQDL
                     GLETVGLETERGFVRVDDRMQTKAKNYYCIGDANGRCLLAHAASAQGETAVENALGHR
                     KELTLPIPSAVYTFPEIASVGLSSRQARRQGIPVRIGEFPVGHLGKAMAVGEETGFVR
                     VIRHFDSGRLLGVHMLGHNATEIIEAAAAVLMVKGSAADLAEMIFAHPTLSEAVKEAA
                     GDAFQSALHLPPRKMMQAAADVHEIR"
     gene            6865..8130
                     /locus_tag="PLE88_08505"
     CDS             6865..8130
                     /locus_tag="PLE88_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76540.1"
                     /translation="MNKHKSEPMGVTDFFLELGLSDEIKAAAGSDPQLNRPIAFNSHI
                     HLPPNFSAFETVQQAVDLAAQQEVRILGVGNYYDFSVYQKFMQAARRRGIFPLFGTEI
                     IALETGLQKKGIRVNDPGNPGKYYICGKGISRFEQLSDRAAELLSIIRRNDALRMRQM
                     TAKLAEAFSRQGVDTGLDEQAIIARVVRRHQCRPETVVLQERHLCQAFQERFFEKVPA
                     AQRTAVLSALFGTAPKAQPEDAVGIQNEIRSHLMKAGKSCFVPEAFVSLAEAKELIAQ
                     LGGIACYPVLADGAKQRCEYETPVETLVETLKAENYRMVELIPIRNHPDVLIEYVTAI
                     RQAGIAVVAGTEHNTLDLLPMEPACVNGQPIPETVKAIFQEGICVLAAHQFLSAHRQA
                     GFDTAGGSPQQRIETFRKIGIAVLNRYFR"
     gene            8175..9257
                     /locus_tag="PLE88_08510"
     CDS             8175..9257
                     /locus_tag="PLE88_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM76541.1"
                     /translation="MTKKINIIAPVLRMLLRRQGRCPVVRYAKSPAADPLLKIAIAPD
                     ADTAAVIEQLRTAVCGQPRPVEAVQIEGVGCFEIVWEEVPPAGRAAGLAAVVTGAAQG
                     FGLEISQHMVQQGARVVLADINLPAAQEEARLLTDAYGPDRAMAVAMDVTNPESIQHA
                     LSEVVAAWGGLDVFISNAGVLKAGSVKTQPKKDFDFVTDVNYKGYFYCVQAVSPLLAV
                     QHQVCPDYTSDIIQINSKSGLVGSNRNGAYAGSKFGGIGLTQSFALELVEDGIKVNSI
                     CPGNFFDGPLWSNPENGLFVQYLRAGKIPGAKTIADVRRAYEEKVPMKRGCTTADVMA
                     AIYYLIEQKYETGQALPVTGGQVMLH"
     gene            9254..11029
                     /locus_tag="PLE88_08515"
     CDS             9254..11029
                     /locus_tag="PLE88_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOM76542.1"
                     /translation="MTSSMGGSNRVDSLYKVQNYLLQKKKEWKKMNGIPEKQTAVQLI
                     GPDKLMLNSAKPVDKPGPYQILARVEAVGLCFSDLKLLKQFDKHARKSEIIAGIDPAV
                     LTDIPSYRPGTEPTVPGHEAFCTIAAVGRKVRRHWVGQRVLVQTDYRWLKTAESNSAF
                     GYNFEGALQQYVLMDERIIVDPQTGESFLIPVEKDLSASAIALVEPWACVESSYATEE
                     RNAVLAAGRLLVAAEAGRKIEGLLESFSPQGRPAAITAVCADESQYELLKGLGVKLTR
                     ANAVSLLPDEAFDDIVYFGSSPATIEILNDKLAARGIINIVLAGRPIGQDVSVGVGRI
                     HYGLTRWIGTTGANAAESYKHIPSVGEVRRGDRVLIAGAAGPMGQMHTIRLICSGIEN
                     LSITGTDFDDQRLEELAHKSAPLAQQRSVHLELVNPNKQPPQGKFSYFAIMAPAGALV
                     AQAIKNSTEGTLINVFAGIPASVRQGVDLDTYIANRCFIFGTSGSRLSDMKAVLQKVL
                     SGQLDTNSSVGAVSGLAGAIEGMRAVENRTIAGKILVYPALEAMPLITLPQLEQVYPS
                     VARKLDNGIWTKSAEEELFRVGRKV"
     gene            11083..12795
                     /locus_tag="PLE88_08520"
     CDS             11083..12795
                     /locus_tag="PLE88_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76543.1"
                     /translation="MAKAQKAECLPEIKKASPRIPVIGVFAPCDPRIDADSRKRAQNI
                     IRMVADAVSGQVVLPDKTPVPVVYSTVLIDSEAQADIVAQQFRTAGVNILVCAPDTWS
                     FPQLTIISLLQQFPKDTPLNITCGNSGPKPGVVYAHAAAGAISQYGKLVHLNVGNWPD
                     TGMNPVMTKATAEALIDWCYAAVTAAALKGRRVVVFGHDSMGMETALAHILPTRNTFG
                     LEITRLDMKLLADMINKGAYNQKELSELRGWIDASIGKRLELKSQADSQRFNQSLAMY
                     LINRDLIADLNAVGGGFMSQLEWGSDRRGIPMPVADVMESLFNSTFDHNGPKAPLPYA
                     TEADVQGLLTMLFMTWLSGGNPPLFMDFRKVWEGWEIKALAAKLGVKVNPKADYIVKG
                     LVDGDNSGSAAFDWAAKPGASVKEILKQVSMPLADEGYFPGGGNSVTFMTPAGIEGIA
                     GRMAYSALNNKFSLIWDEAYTTSVPAKLGQAMCNLTNPTWPHTFVVPRYASMVEYKQY
                     APANHFHMTWNLPVSRLQYWMDLTGVLNASPWAARPAFIEGKDRPLPLIYHLNGGEAA
                     YKNR"
     gene            complement(13011..13604)
                     /locus_tag="PLE88_08525"
     CDS             complement(13011..13604)
                     /locus_tag="PLE88_08525"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOM76544.1"
                     /translation="MKTLRYRLLIRLRPALRFVKFRIFHLEDSPERIARGAAIGLFFA
                     YLPLLGLQMILAFLTAKWTKANKAVAVMTTWVSNPLTAMAVFYPCYRIGRVVLSLFHK
                     KAEVDAEQLEFILEEHLSFWEMIWHLFSYDYWKHLAAILAKIGLEIFIGGVILGIFIA
                     AAGYWLTYKIIISYRRRKKKRLLPSAGINSGTPDALQ"
     gene            complement(13609..14664)
                     /locus_tag="PLE88_08530"
     CDS             complement(13609..14664)
                     /locus_tag="PLE88_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOM76545.1"
                     /translation="MNRADYHNILIIKPSALGDIVHALPVLSSLRASFPKSRLSWLVR
                     KEFAPLLECAEGLDNIITFDRKEMGRWYRNRRALMSLLELRNQLRAARFDLVLDLQGL
                     LRSAIFAWMTGCPDRIGMREAREGAHLFHTRIIDRPAGTVHLLDYYHSILQAIGASVR
                     LTECPLTVPPAAENSILQKMQLYGLRPKQFLVLIASSAHASKCWPPQHFAAAAESLHK
                     RFGWDTAAVGTAGDKTAIEAIQSCCRIPVANLAGQTSIPELIALFRHSAAVISNDTGP
                     GHIALAVKSPAVLVFGPTNPLRLGPYRRPECIAAIDPDKRGCAVKSSNPAYRIEHVPA
                     EMVIEKILEQIPAVQCL"
     gene            15165..>15580
                     /locus_tag="PLE88_08535"
     CDS             15165..>15580
                     /locus_tag="PLE88_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOM76546.1"
                     /translation="MIVTRFAPSPTGYLHVGGARTALFNWLLARRLGGKFLLRIEDTD
                     LKRNTPTATAQVLKDLHWLGLDWDEGPDVGGPNGPYLQSQRRAIYDHYIRKLLDEQKA
                     YYCFDTPQELEEMRSRAEAEKRHNLYPRPEQFPTEA"
     assembly_gap    15581..15590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15591..>15854
                     /locus_tag="PLE88_08540"
     CDS             <15591..>15854
                     /locus_tag="PLE88_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOM76547.1"
                     /translation="AQKARREGRPVTVRFAVTQTEPIVIDDEIRGQVTFNPAEIGDFI
                     ILKSDGFPTYHFAVVVDDELMGVTHVIRGQEHLMNTPNHQLLQ"
BASE COUNT         3827 a         3922 c         4432 g         3663 t
ORIGIN      
        1 tgtacagaaa atccgaagga tctcggtcgc ctttggttga aactgcttca aaagtaatga
       61 agcaatccgg caaaagctgc tggagcatcc ggccaaccga ttcggcctga gccatcgcca
      121 atttactggc acgggttgcg attttcaaat gccgtttcac atcaaagacc tcgtctgctg
      181 catccaccag acggctattg taggcataaa accgattgat ttcaaggatt taagattaat
      241 aggccccccg tgcagcaatc gccccgcacc ttggatagat tgttgcccga tacagcgctg
      301 gttaatccgt ctgatggcct tataaagcag ccttgcttcg gcgtatcagt tctgctgccg
      361 agcagggacg cttaacctga taccctttca gcggaagaat gtgctcgtga atcatatcca
      421 gaaaatcaga cggatacggg aagaaagcat cttcgacctc atcgggcgga gtgatccagt
      481 tgcgtgcgcc gaggacaacc ggcggagcat ccaattcatc gaatgccagc agaccgatgg
      541 tcgaagccat cgtatgcaga aacgaacccc gctgacaggc atcggaagcc agaagtatct
      601 tgcgggtctt tttaaccgat tcgatgactg gttcataatt gaacgggacc agggagcggg
      661 catcgatgac ctcacaggtt aagccccagt tttgctctaa ttgcttggca gcttccagtg
      721 cccgatacag cgttgcaccg atagtgagaa tagtcaggtc cctgccttcc cggcggacag
      781 ccggctggcc gaacggcact tcataataat ccagcggaac cccgccttgg acgaattcct
      841 cggctatgcc gtaaagccgc tggctttcaa agaaaataac cggatctgtg cccgttagag
      901 ccgcatacat cagtcctttg gcatcatagg gcgtggccgg aaaaacgact ttcaacccgg
      961 gaatatgggc acacagcgag gtccagtcct gactgtgctg ggcgccgtat ttgctgccga
     1021 cggacacgcg tacgaccacc ggcattttca gcagaccacc gctcatcgat tgccatttgc
     1081 acaattggtt gaatatttcg tccccggccc gtcccagaaa atcgcagtac atcagctcga
     1141 ccagtgcccg accgccttcc agagcatagc cgacagcggt gcccacgatg gcgccttcgg
     1201 caataggagc attaaacagc cgatggtaag gcagggcttc cgtcaggccg cggtacaccg
     1261 caaaggcccc gccccaatcc ctgttttctt ccccgtaagc caccaaagtg gggtccgcat
     1321 agaagcggtc gagaatggcc tcaaacaaag catcgcgcag aacgacgcag cggctgtcct
     1381 taagccgctg gccggtttca tcaaatgccg accggcttcg ggcggtcagc gcctgaacgc
     1441 ggggattttg gtctttgctc agaagcactt ccggctttct gcccggttcc atggaatcca
     1501 ttttctgatt ggaatacatt gtctggtcga gcagacagtt ggcttttttc aggtctgcac
     1561 ggggggagat ggttttatct actgctttgc ggcaggcctt gatgataagc tgctcgacat
     1621 gctgtctgat tgcgtcaatg gcttgatcct cgcagatgcc ggcggcagca agttctttgg
     1681 caaaatgaac aattgggtca tgctgctgcc atgcctccac ttcggttttc tcgcggtagc
     1741 tgctttggtc ggaaggtgaa tggccgctgt agcggtaggt gatggtgtcc agcagcacgg
     1801 ggccgtctcc ctgttcaaga atctgacgct tgcgcaggac agcatcggca accgccagcg
     1861 gattatagcc gtcgatgcgt tcggcatgca tctgctgcgg attcaggccg gctccgagcc
     1921 gtgccagcat cccaaagccc atcgtctcgc cttccggctg gccgcccatg ccgtagaaat
     1981 tgttgttgaa attgacaatc agcggcagcc cgccgcggtg tgcttcgtcc cagagtgttt
     2041 taaattgctc cattgtcgca aagcacagcc cttcccagac aggcccgcag gaagacgatg
     2101 cgtcgccgat gttggcaatg actatgccgc tttttttatt cacttttttg tagagggctg
     2161 cccccactgc gatatcgcct gagccgccga caatcgcatt gttgggatag atgccaaacg
     2221 gcgggaaaaa ggcatgcatg gagccgccca ttcccttgtt gaagccggct tcccggccga
     2281 atatctcggc cagcgtgccg taaatgagga aatcaatcgc cagatccttc actgagccgg
     2341 cagcgtcttt ttcgacaatt ttgagacact gcccgtcaaa atagtttttc attatctgca
     2401 tcaatgccgc atcatccagc ttttgaatag cggacagccc tttggccaaa atctcgccgt
     2461 gactgcggtg gctgccgtaa atatggtcat cgatacccag atgaaatgcc tgaccgacag
     2521 cggcggcttc ctgtccgata gacagatgtg ccggtccatt gtgcatatac tcgataccca
     2581 tgtaggagcc gcggagcttg atttcattga gcatcgtttc aaaggtgcgc agaatcagca
     2641 tatcccgctg aatgcgtacc aagtcttctt tggtgtagcg gctgagttca tccctgacgg
     2701 ttttgctgta ttgattaacc ggaatcggcg taaattgaat aaatgtggct ttgcgttttt
     2761 cgttgggatc gaccattaaa catttcggca tttgtgctgc tcctgattat ctattaagaa
     2821 cctgttgtta agagtgattg tgagacgccg gcgtctggga agagcacggc aatgtttttt
     2881 ttctccagat aggcacacca ttgttcggag ggccgacggt cggttataat ggtgctgagg
     2941 cagtcaaagt cggcgatttt gtacagggat ggacttccga acttgctgtg gtcggccagc
     3001 agaacaactt gacgggcatt gcgggcggcc agcccaaaca ggtgggcact ttctatatcg
     3061 atagaagtca gcccaaagtc cattcccaat ccatcggtgc ccagaaacgc acaatagccg
     3121 cgcagccggc ctaatgtttc agctgccagc gggccgaccg tgtccattcg ctccggacgg
     3181 tactgccctc cgagcatcag gactttcagt gtcggagtga aaagctcctg agccgtgcgt
     3241 atcgaattga caatgaccga caaactgcgt ttgtttcgca agaaaggggt catttcaaaa
     3301 caggtagttc cggagtcgag aaatatctgt tcaccgtcag caatcatctg gctggccatt
     3361 ttggcaattg ttcgtttggc ttcaacgttg cgcatcgatt tggaaataaa cgaataatcg
     3421 gaattgcgga caacagatgc acccccgtga gtcagctcca gcagaccttc ggcggctaat
     3481 ccctgcaaat cccgccgaat cgtggcttcg gagacttgaa gttcctcagc cagccggcgg
     3541 acagaaacat ggccgagctg ataaacccag ttcataacag cttgtcgtct ttgctggatt
     3601 gtgtatgtca taattatcgg gctttgattt ttttgataaa tctaagatgc tataataaag
     3661 cataaaaaca gaaatgcaac aagattttgc aatattatgc ttgatttatg aaataatttg
     3721 gtgtattttt gaaatattat aatgaatatt acataaacgg agagccctat gtttgttata
     3781 aaatatccct caaaagggat tcctgcatca gaaggtgtag tggcggcttg gacagctagt
     3841 gagggaaaac ttgttaaaaa gggcgaaatt ctggctgaaa ttgagatttc gagtgccgtc
     3901 atagcattag aaagttccat aagcggcaca ttgaggaaaa taatggctcc cgacggcaca
     3961 ccggtaaagg tgggagatgc tttggcactt gtgggccgtc cagaagaaga catttcgagt
     4021 attctttcaa aaatcactcc gccgttgcct gctgtcgatg aaccaaaata tgcaaagccc
     4081 gaaacttctg cgggcgtttc ggtggaaagc caaaaagacg acaaaagaca accgaatata
     4141 ttaaagaagg aagaaaatat gacacttgcc aagccgatcc agcctgccgc caattcaggg
     4201 ggcattattc ctgttttaat gccgcaggcc ggccagacga tggaggaagg cacgattttg
     4261 tcgtggaagg ttaaggaagg cgatagaatt gcggttggtc aggtaataat ggagattgag
     4321 acggacaagg cggccatgga agttgaagcg gtcgatgctg gccgaattgc aaaaatcatt
     4381 gctcctgagg gtacagttgt gccggtaaag accccggttg cttatctggc agaggaaggg
     4441 atcgatattg atgcttttct tgcctcagga gcggatgctt cagtgccttc ggtacagtct
     4501 gccgacacga tggcggcacc agctcagtcc agagatacag aatcatcccg acagccggct
     4561 tctgtcagtg aaaccgggcg agttaaggcc tcgccggctg cccgcaaaat ggcacgggat
     4621 aagggcattg accttgccgg cctgaccgcc ggctcagggc ccggcggacg cattctttcc
     4681 gccgatgtgc aggcggtaca agcggttcct tctcaagtac agacatatgc ggtcgacaaa
     4741 atgcggcagg ccattgccaa caatctattg tattccaaac agaatatccc gcatttttat
     4801 gccaaattga ctcttgatgc cggcagcttg tatgccgtct atcgcaagac aaaggagcag
     4861 ttcaagtgca gtatcaatga tttcataact gccgcgtgtg caagggcaat ccgtcagtat
     4921 ccggcttttc ggagccaata tagggatggg cagattgttg aaaatccggc cgtcaatatc
     4981 ggcattgctg taggaacgga aaatggactg actgtaccgg ttgtgctgaa tgccgaccgg
     5041 atgaatttgg aacaattggc atccagcagc cgtcaagtcg tagaagctgc ccgcagcggc
     5101 aagctcaaag gggttggaca aggcgtattt accatcacca atttggggat gtttggagtg
     5161 gaggaatttg cagccattat aaatccgcct gaatcggcga ttctggctgt gggagctctt
     5221 cgcgagggaa cagctgttga aaacggagcc atccgtgcaa ctcggctgat gacgattacc
     5281 ctgagtgtgg atcatcgtgt aatagatggg gttttggcag ctgagtttct taaaactgtc
     5341 aaagagctcc tggaaaaacc ggaacaattg atttaatatc gcacggcaag gataacgtta
     5401 tgactgagca ttatcaaata gccgttatcg gagcaggacc cggcgggtat atcgccgcac
     5461 ttaaagccgc tcggatgggg gccaaaacgg cgattgtgga aaagcatcat gtgggcggga
     5521 cctgtctgaa ttacggctgt atcccttcca aagcactgct ggcttcggca gaactgcttc
     5581 accgcatacg caaagcagat tcattgggca ttcgggtaaa cggggcggtc agttttgact
     5641 ggccgtctat ccagaagcgg aaagacaaag tcctgatgaa gctgcggggc ggaattaagg
     5701 ggcttttcgc ggccgctgga gtcaccctgc atagcggacg ggcagttctt gacgggccgg
     5761 gcaggattgc tgttctggat gagcaaggtt cagcggtttc ttttacagca gacagcgtta
     5821 ttttggcgcc gggctcgata ccggctcgta ttcccggctg gccgacagat acgcatcttg
     5881 tctgtacatc cgatgaagcc ctgcactgga aagatttacc ccaacggctg ctgattgtcg
     5941 gaggcggcgt tatcggctgc gagtttgcct gtatgatgca ggagtacggc gtcgaggtgg
     6001 ccatagtgga aatgctggat tccctgctgc cggagatgga gacagcgctg ggggcggctc
     6061 ttaaagatat ttttatccgg cgcggaattg ctgtgtatac aggcagcaag gttgacaatc
     6121 tgtctgtcaa agacggctct gtggaggcgg tcttgtcagg cggtcaggtc attaaagcag
     6181 atcgggtgct tacagctgcc ggacgaaagc cggcggtgca ggatttggga ttggaaacag
     6241 tcggactgga gaccgaaagg ggctttgtcc gtgtggatga ccgaatgcag acgaaagcaa
     6301 agaattatta ctgcattggt gatgccaacg ggcgatgcct gctggctcat gctgccagtg
     6361 cgcagggcga gacagctgta gaaaacgcct tgggacaccg caaagaactg acgctgccga
     6421 ttccaagtgc tgtgtatacc tttcctgaaa ttgcctctgt cggcctgtcg agtcgtcagg
     6481 cccgccggca gggtattccg gttcgcatcg gtgaatttcc tgttggccat ctgggcaagg
     6541 cgatggcggt tggtgaagag acaggttttg ttcgggtgat tcgccatttt gacagcggcc
     6601 ggctgctggg cgtgcatatg ctcggccaca atgccaccga aatcattgag gcggctgcgg
     6661 ctgtgctgat ggtaaaaggc agcgccgcgg atttggcgga aatgattttt gcacatccga
     6721 cattatcgga agcggtcaag gaagctgccg gcgatgcatt tcagtcggct ctgcatctgc
     6781 cgccccgcaa gatgatgcag gccgccgcgg atgttcacga gatacggtaa tcaagccaag
     6841 aagaatgatt ggtcaagagt gaagatgaat aagcacaagt cagaaccgat gggagtaacg
     6901 gatttttttc tggaactggg cttatcggat gaaatcaaag ccgcagcagg cagcgatccc
     6961 cagcttaatc ggcctattgc cttcaacagc catattcact tgcccccgaa tttttcggca
     7021 tttgaaacag tccagcaggc cgttgacctt gcagcccagc aggaggtgcg gattttgggc
     7081 gtcgggaatt attatgattt ttctgtctat cagaagttta tgcaggctgc ccgacgcaga
     7141 gggattttcc cgttgtttgg gacagaaatt atcgcccttg aaaccggcct gcagaagaag
     7201 ggcattcgcg tcaatgatcc cggcaatccc ggcaaatatt atatctgcgg caaaggcatc
     7261 agccggtttg aacagctttc ggacagagct gcggagctgc taagcattat tcgcaggaac
     7321 gatgccctgc ggatgcggca gatgacagcc aagctggcag aggcattcag ccgtcaggga
     7381 gtggataccg gcctcgatga gcaggcaatt atcgcccgtg ttgtccgtcg ccatcagtgc
     7441 cgtcccgaaa cagtggtttt gcaggaacgg catctctgtc aggcgtttca ggagcgtttt
     7501 tttgagaagg tccccgcagc acagcggacg gcggtgttga gtgccctttt tgggactgca
     7561 cccaaggccc agccggaaga tgccgtaggc attcagaacg aaatccgttc ccatttgatg
     7621 aaggccggca agtcctgttt tgtgccggag gcctttgtca gtttggctga agccaaagag
     7681 ctgatagccc agctgggcgg aatagcctgt tatccggttc tggcagacgg agctaaacag
     7741 cggtgcgaat acgaaactcc ggttgaaacg ctggttgaaa cgctcaaggc cgaaaattac
     7801 aggatggttg aattgattcc gattcgaaat caccctgatg ttttaatcga atatgttaca
     7861 gcgattcgcc aagccggcat tgccgtggta gccggcacgg agcataatac gctggatctg
     7921 ctgccgatgg aaccggcctg cgtcaatggc cagccgattc ccgaaacggt caaagcaatt
     7981 ttccaagagg gcatctgtgt gctggctgca catcagtttt taagtgctca tcgccaggca
     8041 ggttttgaca cggcgggggg atcccctcag cagcgcatcg agacatttcg aaaaatagga
     8101 atagctgttc tgaaccggta ttttcggtaa gacagcagat tggatatgag gttttggaag
     8161 cgatggatca ggagatgacg aaaaaaataa atataatagc accggtgctg cggatgcttc
     8221 tccgccgaca aggacgctgt ccggtggtcc gatatgcaaa atccccggca gcggacccgc
     8281 tcttaaagat tgcaattgcc ccagatgctg atacagccgc tgtgattgaa cagcttcgga
     8341 cggcagtttg cggccagccg agaccggtcg aagccgtgca gattgaaggt gtcggctgtt
     8401 ttgaaattgt ttgggaagaa gtgccgcctg ctggacgggc ggcggggctg gcggctgtag
     8461 tgacaggagc agcgcagggc ttcggactgg agatttcgca gcatatggtt cagcagggcg
     8521 cccgggtggt attggccgat attaatctgc cggcagccca agaagaagcc cgcctgctga
     8581 ccgatgccta cggaccggac cgggcgatgg cggtggctat ggatgtgacc aatccggagt
     8641 ccatccaaca tgcactcagc gaagttgttg cagcatgggg tggattggat gtgtttatct
     8701 ccaatgccgg tgtgttgaag gccggcagcg tcaaaaccca gccgaagaag gattttgatt
     8761 ttgtaaccga tgtcaattac aagggttatt tctactgtgt acaggcggta tcgcctctgc
     8821 tggcagtgca gcatcaggtc tgtcccgatt ataccagcga tatcattcaa atcaattcca
     8881 agtcggggct ggttggctcc aaccgcaacg gggcatatgc cggcagcaaa ttcggcggca
     8941 tcgggctgac acagtcgttt gcccttgagc tggtggagga cggcatcaaa gtgaattcta
     9001 tctgtccggg caatttcttt gatgggccgc tgtggtccaa tccggaaaac ggactgtttg
     9061 ttcagtatct gcgagccggc aaaatccccg gcgctaagac catcgccgat gtgcgtcggg
     9121 cctatgagga aaaggtgccg atgaagcggg gctgtacaac agccgatgtg atggcggcga
     9181 tctattatct gatagagcag aagtatgaaa ccggccaggc cttgcctgtt accggcgggc
     9241 aggtcatgct gcattgacaa gctcgatggg cggaagcaat cgtgttgatt cattatataa
     9301 agtgcagaac tatctgctgc agaaaaagaa agaatggaag aagatgaatg gcattcccga
     9361 aaaacagacg gcagtccaat taatcgggcc tgacaagctg atgctgaatt cagccaaacc
     9421 ggtcgataag ccggggccct atcagattct ggctcgggtg gaggccgtgg gtttgtgttt
     9481 ttccgatttg aagctgctca agcagtttga caaacacgcc cgcaagagcg agattattgc
     9541 cggcatcgat ccggctgttc taaccgatat ccccagctac cgccccggca cagagcccac
     9601 agtgcccgga catgaggcct tttgtacgat tgcggctgtt ggccgcaagg tccggcggca
     9661 ttgggtcggg cagcgtgttc tggtgcagac cgattaccgc tggcttaaaa ctgccgaatc
     9721 caactcggca ttcgggtata actttgaagg ggcgctgcag cagtatgttc tgatggatga
     9781 acggattatt gttgatcccc aaaccggcga aagttttttg attccggttg agaaagacct
     9841 cagtgcttct gctatcgcac tggtggagcc gtgggcgtgc gtggaaagtt cgtatgccac
     9901 ggaagagcga aatgccgtac tggcggcagg cagactgctg gttgctgccg aagcgggcag
     9961 aaagattgaa gggctcttgg aaagtttttc gccgcagggc agacctgctg cgattaccgc
    10021 cgtctgtgct gacgagagcc aatacgaact gctcaaagga ctgggcgtca aactgacgcg
    10081 tgccaatgct gtgtcgctgc tgcctgatga ggcatttgat gatattgtct atttcggctc
    10141 atcaccggcg acgattgaaa ttctcaacga caagctggcg gcccggggga ttatcaatat
    10201 tgtgctggcc ggccggccga ttgggcagga tgtatcggtc ggcgtcggcc ggatacatta
    10261 tggattgacc cggtggatcg gcacaacggg ggccaatgca gccgaaagct acaagcatat
    10321 tccctctgtt ggggaagtcc ggcgggggga ccgggtgctg attgcgggag ctgcaggccc
    10381 catgggacag atgcatacca ttcgcctcat ttgttcgggc attgaaaacc tgtcgattac
    10441 agggacagat tttgatgatc aacggctgga ggaactggct cataagtctg caccgctggc
    10501 tcagcagcgc agcgtgcatc tcgagctggt caatccgaac aaacagcccc cgcagggcaa
    10561 attcagttat tttgcgatta tggcgccggc aggggcgctg gtggcgcagg ccatcaaaaa
    10621 cagcaccgaa gggacgctga tcaatgtctt tgcgggcatt cccgcctctg tccggcaggg
    10681 tgtggatctg gatacttata ttgccaatcg atgctttatt tttggaacca gcggctcgcg
    10741 tttgtccgat atgaaggctg tgctccaaaa agtcctgtca ggacagctgg ataccaacag
    10801 ttccgtcggc gctgtcagcg gcttggccgg agcgattgaa gggatgcggg cggttgaaaa
    10861 ccgcaccatt gcaggtaaaa tcctcgttta ccccgccctt gaagcgatgc ctttaatcac
    10921 gctgccgcag cttgagcagg tctatccttc tgttgcccga aagctggaca acggaatttg
    10981 gaccaaaagc gccgaagagg agctttttcg tgttggacgc aaggtctgat gcggtatata
    11041 ataatggtgc aggataaacc gatttttgga aaggaaacag gtatggcaaa agcgcagaaa
    11101 gcggaatgtt tacccgaaat caagaaagca tctccccgaa tcccagtcat cggtgttttt
    11161 gcgccctgcg atccgcgcat cgatgccgac agccggaaac gtgcccagaa tattatcagg
    11221 atggttgctg atgctgtcag cggtcaggtg gtattgcccg ataaaacgcc ggtgccggta
    11281 gtctattcaa ctgtcctaat cgacagcgag gcccaagccg atatcgtcgc ccagcagttc
    11341 cgcaccgccg gcgtgaatat tctggtctgt gcccccgata catggtcgtt tccgcagctg
    11401 acgattattt cgcttttgca gcagtttccc aaagatacgc cgctgaatat tacctgcggc
    11461 aacagcggcc ccaagcccgg tgtggtctat gctcatgcgg cggcaggtgc tatcagccaa
    11521 tacggcaagc tggtgcatct gaatgtgggc aactggccgg ataccggaat gaatccggtg
    11581 atgaccaaag ccacggccga agcgctgatt gactggtgct atgcggctgt aacggcagcg
    11641 gcgctgaaag gcagacgcgt ggttgttttc ggccatgatt cgatggggat ggagacggcg
    11701 ctggcgcata ttctgccgac acgaaatacg ttcggactgg aaattacccg tctggatatg
    11761 aaactgctgg cggatatgat aaacaaaggc gcctacaatc agaaggagct cagtgagctt
    11821 cgaggctgga ttgatgcgtc tatcggcaag cggctggagc tgaaaagcca ggccgacagc
    11881 cagcgcttca accagagctt ggcgatgtat ttgataaacc gcgaccttat cgctgacctg
    11941 aatgcggtcg gcggcggctt tatgagccag ctggaatggg gcagcgaccg gcggggcatt
    12001 cccatgccgg tagcagatgt aatggagtcg ctgtttaaca gcacctttga ccacaacggc
    12061 cccaaggctc cgctgccgta cgcaacggag gccgatgtgc agggattgct gaccatgctg
    12121 tttatgacgt ggctcagcgg cggcaatccg cctctgttta tggacttccg caaggtctgg
    12181 gaagggtggg aaatcaaggc cttagccgcc aagctgggcg tgaaggtcaa tcccaaggcc
    12241 gattatattg tcaagggcct tgtggacggc gacaactccg gcagtgcggc gtttgactgg
    12301 gccgccaagc ccggcgcatc ggtcaaggag attctgaagc aggtgtcgat gcctctggcc
    12361 gatgaaggct acttccccgg cggtggcaat tccgtcacat ttatgacgcc ggcaggcatc
    12421 gaaggcatcg ccggacgtat ggcctacagc gccttgaaca acaaattcag tctgatctgg
    12481 gatgaggcct ataccaccag tgttccagcc aagctggggc aggctatgtg caatctgacc
    12541 aatccgacat ggccgcacac attcgtggtg ccgcgctatg ccagcatggt cgaatacaag
    12601 cagtatgcgc cggccaacca cttccatatg acatggaatc tgccggtcag ccggctgcag
    12661 tactggatgg acttgacagg tgtgctgaac gcctcaccgt gggcggcccg accggccttc
    12721 atcgaaggca aagaccggcc gctgcctttg atttatcatc tcaacggcgg cgaggcggcc
    12781 tacaaaaacc gctgatgcaa tacggctctt tcggcagcag cgcttgtaag cagccggaat
    12841 atacagctgt attcgcaaac ggcagggtgt tatgtgcacc ctgccgtttg ctgttatctg
    12901 ctgtttggcc gactgaaaca gcaaaagcac tgttgagacg aaatgaatgg gcggaatcaa
    12961 tggtcttgtt tgcaggatat ttctatcttt aaagtgacta ctatcctatt tcattgaagt
    13021 gcatccgggg tgcctgagtt tattcctgct gatggcaaga ggcgtttttt cttccgccgg
    13081 cgatagctga taattatctt ataggtcagc caataccctg ctgctgcaat aaaaattccg
    13141 agaatcacac cgccgatgaa tatttctaat ccaatcttgg caaggatagc tgccaaatgc
    13201 ttccagtagt cataggaaaa caaatgccaa atcatctccc agaaggaaag atgctcttct
    13261 aagataaatt ccaattgttc agcatcgact tcggcctttt tgtgaaacag cgacagcacg
    13321 actctgccaa tgcggtagca ggggtaaaat acggccatag ctgtcagtgg attgctgacc
    13381 catgtggtca tcacggcgac agctttgttg gcttttgtcc acttggccgt cagaaaggca
    13441 agaatcattt gcagacccag cagaggcaga taggcgaaaa atagtccgat tgcagccccg
    13501 cgggcgattc gctccgggga atcttcaagg tgaaagatgc gaaatttgac aaatcgcaac
    13561 gccggacgca gccgtatcaa tagtcggtat cgcagagtct tcattcattc acaaacactg
    13621 aacggcaggt atttgttcga gaattttttc aatgaccatc tccgccggca catgctcaat
    13681 ccgatacgcg ggattggaac ttttcacggc acagccgcgt ttgtcaggat caatagcggc
    13741 gatgcactcg ggtcggcggt aggggcctaa ccgaagggga ttggttggcc caaagaccag
    13801 tacagccgga cttttaactg ccaaggcaat atgtccggga ccggtgtcat tggagataac
    13861 cgccgcagaa tgccgaaata gggcaattaa ttccgggata ctggtttgac cggctaaatt
    13921 tgccaccggt atcctgcagc agctctgaat ggcttctatt gccgttttat cgccggcagt
    13981 accaacggca gcagtatccc agccgaaacg cttatgaagt gattccgcgg cggcggcaaa
    14041 atgctgcggc ggccaacatt tagaagcatg ggcagaactg gcaatcagaa cgagaaattg
    14101 cttggggcgc agaccataca gctgcatttt ctgcaagatt gaattttccg ccgccggcgg
    14161 gacggttaaa ggacattctg tcaatcgtac agatgcgccg atggcctgca gaatgctgtg
    14221 atagtaatcc agcaagtgta cagttccggc gggccggtct ataatccgag tatggaacaa
    14281 atgagcccct tcacgggcct cccgcatccc gatccggtca ggacagccgg tcatccacgc
    14341 aaatatagcg cttcgaagca gaccttgcag gtccaataca agatcaaatc tggctgctcg
    14401 cagctggttt cgaagctcca acagagacat caacgcccgc ctattgcgat accatcgccc
    14461 catttctttc cggtcaaagg taatgatatt gtcaagtccc tccgcacact ccagcagagg
    14521 agcaaattcc ttccgcacaa gccagcttag acggcttttc ggaaatgaag cacgcagcga
    14581 acttaaaaca ggcagtgcat ggacaatatc ccccagagca ctgggtttaa taataagaat
    14641 attgtgatag tctgctctat tcatattttg agaattttta cgcctatcca gattgggcga
    14701 tattctattc aataagccaa tgattgcaac agcatatcct gctgcatatt aagattaaaa
    14761 tcttcaaaat tatataaaaa atatccaatt ttttcgggaa atagtatgac caaagacgac
    14821 tatgatttgg tttctgccag cagctgccaa cagcaattgt aattgtacaa agcaattata
    14881 tagagccaga aactcctata tttggaatat ttttcgttta attaaaacca tgtcaaaaag
    14941 tataatcctc ttgtaaactg cgtaaataaa ggacaaaaaa ggctgaaaat agcattaaat
    15001 acgatacaga ttagcgttat ttgtcggcaa aaccaagccc tgtaatttct gaatccaatc
    15061 tcgatccaag tacagggaaa tgtaatttgt gttgttcttg actttgcagg atgggtcaag
    15121 tacactcctt tctttccatt taactgacaa aagatgaggt tgttatgata gtaacgcggt
    15181 ttgcaccttc cccgactgga tacctgcatg tcggcggagc cagaacggct ttattcaatt
    15241 ggctgctggc ccggcgcctt ggcgggaaat tcctgctgcg gattgaagat accgacttaa
    15301 aacggaatac gcctacggcg actgcccaag ttcttaaaga cctgcactgg ctgggcttag
    15361 attgggacga aggccccgat gtgggggggc caaacggacc gtatctgcag tcgcagcggc
    15421 gcgccattta tgaccattac atccgcaagc tcttggatga gcagaaggct tattattgtt
    15481 ttgatacgcc ccaagagctg gaagaaatgc gaagccgtgc cgaagcagaa aaacggcata
    15541 atctatatcc ccggccggaa caatttccca ccgaagccga nnnnnnnnnn atgcacaaaa
    15601 agcccgccga gaagggcggc cggtaaccgt gcggtttgct gtgacgcaga cagagccgat
    15661 tgtgatagat gatgaaatcc gcgggcaggt tacctttaat ccggctgaaa tcggcgattt
    15721 tatcatcctc aaaagtgacg gctttccaac ctatcacttt gcggtggttg tggacgatga
    15781 gctgatgggg gtgacgcatg tcatccgcgg gcaggaacat ttgatgaata ctcccaatca
    15841 ccagcttttg caga
//
LOCUS       DAOTBO010000067        15765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3109_length_15765_cov_5.598982, whole genome shotgun sequence.
ACCESSION   DAOTBO010000067 DAOTBO010000000
VERSION     DAOTBO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_15765_cov_5.598982"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLE88_08545"
     CDS             <1..413
                     /locus_tag="PLE88_08545"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOM76548.1"
                     /translation="QIICGADSLNNIIEDTVVAAMEPQNVLQHIHDNSIIIIPGDRVD
                     NILVSILVLSRGQPSGGIILTGGFEPHPMIEPLLKSSRIPVLLSQEDTFTVSARMKDL
                     GFKIRSYDTEKVARLHDLVDKYVDCGFILNAVQQ"
     gene            616..816
                     /locus_tag="PLE88_08550"
     CDS             616..816
                     /locus_tag="PLE88_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76549.1"
                     /translation="MEVRIMHTHIHRPEWNKIKLQMDDILRDPRFWAVLVLIVLFGLM
                     VLTALFTKQGSGTIPVHPFYMP"
     gene            901..1419
                     /locus_tag="PLE88_08555"
     CDS             901..1419
                     /locus_tag="PLE88_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOM76550.1"
                     /translation="MSVQEYLQQHHCGYRVTEHKPVFTAEQLAVVEHVSPRQVAKPVV
                     VRCDGQYYLCVLPADRKIDFYAIGHHLKVKNVDLASEQEMKTLFTDSELGAEPPFGNL
                     YNLPTLMDKTLAKDKEIVFQAGSHKQSLWMSMDDYKKLTNPDIFSFSYPQVLDEIESM
                     PFDPFFYDPYGI"
     gene            1695..2315
                     /locus_tag="PLE88_08560"
     CDS             1695..2315
                     /locus_tag="PLE88_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76551.1"
                     /translation="MADEQVLVIKRSIIEEIGVFHGLMFNIREYLDRIFAAGAGQFIP
                     RTQAEKDPGFKQLIPYVLISCSGRYLTYVRGKRAGESRLIAKRSIGIGGHINPTDDMS
                     LFSPYQTYLNAVQREVEEEIYIQARYTDRIAALLNDDTNEVGAVHLGIVHLWTLDSQQ
                     VSRKEQMITQLEFMNKDALRQVREQMETWSQLCLDELDNIEKTVLS"
     gene            complement(2323..3270)
                     /locus_tag="PLE88_08565"
     CDS             complement(2323..3270)
                     /locus_tag="PLE88_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOM76552.1"
                     /translation="MFFWLMLVWLAFGYILFSILERKYPCGLCNFFVRLSAIGLGVGW
                     LCLIVLLSHDLLYLIFRFPLDISRWFAAGTILLLSVYAFMNARCINVETIELPAPVNL
                     NIVQLSDIHLGSVSWRHLERLVEKSAALKPDAVVITGDLIDPQGHLPPDALLPLQRLN
                     VPIYWISGNHERYFGLEKAAALLKQTAVIPLRNEAVKWSGIQLVGIDDSDDPAWVEHM
                     LTRLQRQPDLFTVLLYHQPEGYNAAARAGIDLMLCGHTHNGQIWPFNWVVRSRFQYLK
                     GLHRIGRMHLYISTGSGTWGPPMRLGSRNQITFICLRNS"
     gene            3600..3980
                     /locus_tag="PLE88_08570"
     CDS             3600..3980
                     /locus_tag="PLE88_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOM76553.1"
                     /translation="MNLNEYFENTDGVGILATADSKGNVDCAIYATPHIIDEETIAFI
                     MRPRLSYNNIRQNPKAVYMFIEKGAGYKGRRLYLEMSHVETNSEKVHLIRRKSHGGGD
                     EADAKLVYFKVMLTRPLVGEQETS"
     gene            3982..4461
                     /locus_tag="PLE88_08575"
     CDS             3982..4461
                     /locus_tag="PLE88_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOM76554.1"
                     /translation="MNRQLLLLCTICILICLAVGGISGLTVQNGGGEWYAALKRPPLN
                     PPAQVFGIVWPILYVLMGIAAGLIWARGWQTAGVKQALLVFGIQLVLNGLWSPLFFGL
                     HWIGAALAECILLWAMVLAAAARFKTICPSASLLLWPYLGWLTFAVYLNAGYWILNR"
     gene            complement(4530..5207)
                     /locus_tag="PLE88_08580"
     CDS             complement(4530..5207)
                     /locus_tag="PLE88_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOM76555.1"
                     /translation="MKRFVVIGLGRFGSSLARALSAGGAEVIAIDRDREVIENIRDQV
                     AVAIRLDSTDIDALKAQGIDRVDIAVVGMGAGHGFESAILTVVNLKKLGIPQIFARAE
                     NTTAGTVFTAIGATEVIYPEIETAQRWAYRLITPHIEEKIDFSPGYSLARVSAPASFD
                     GKTVRELQLRQKYNLNLVAIKRSRAVEQEESGEILNVPMPETKVYQDDILMVAGADAD
                     LARLPQE"
     gene            complement(5279..7048)
                     /locus_tag="PLE88_08585"
     CDS             complement(5279..7048)
                     /locus_tag="PLE88_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOM76556.1"
                     /translation="MKVEYKNKKLDAVIVGLNLIGIAAVASTFVMLYGFDQPPLPSIW
                     LHSAEIILFCFFVTEKLIRYFNAVSKRDFFRLYWFEIPLLGLLFVLFLGAEKWSSGQI
                     FEWFVQGISIYLIVQVIDKVCRSTVYLASTGHNPARSLITLFVVLILAGAGLLMLPRA
                     HEGTINVTDAVFTATSATCVTGLIVKNTGRDFTLFGQIVILSLMQLGGLGIVIFGAVL
                     GLLLGQSMTMREQAALQDLLNERTLSRISTMVGFIIVSTLLVEAVGAVCLYPMWDYVP
                     SLVAPERRWFFSIFHSISAFCNAGFSLMSNSLMDFRSCISVYAVICPLIILGGLGFAV
                     LYNLAEVSGQRLNRLFSKKGIYPDVFNLQSPRRLTLQTKVVLTTTLLLIMAGTAGLML
                     FEACSTSPQRDPRFLSALFQSITARTAGFNTIDIAALSPASKLILIILMCIGGSPGST
                     AGGIKTVTFALVVMVIYATLGKRREVEIFRRAVPLVVLGRAVTITVLYAVMLLAGTLA
                     LTITERGESLPIEDVAFEAASAMGTVGLTTGITPVLTTAGKWIIVAMMLIGRLGPMTL
                     LTALMFNVKPAKYDYPVEPLVVG"
     gene            complement(7163..9259)
                     /gene="recG"
                     /locus_tag="PLE88_08590"
     CDS             complement(7163..9259)
                     /gene="recG"
                     /locus_tag="PLE88_08590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOM76557.1"
                     /translation="MVQQAGTITLDTPVQFLAGVGPMRAQAFDELGVKTVGDLLEYYP
                     RDWVFMPEPMAISDVRANQTVCILGLVEQTDWQPYRKIPLFEATLADQTGTIRIVWFH
                     GRYLVNQLEPGQMLLVHGKAIRYRYQLQINNPKFLIVHPDQPRGPDAFSGPVYPASAK
                     LPSWQIKKIVRTNIDLLAPLMPELFDAAFRKRNELMPRPDAYRQIHIPDNEDMLAAAR
                     RRLKYEELFFMQAGLALKRYHIQHACPAVPMVCTDEIDRRIRQRFPFLLTADQEQVIR
                     EITADMAKEVPMNRLLQGDVGSGKTVVALYAALVAVANKTQAAIMAPTEILARQHFAS
                     IERFLAGSRVRRELLIGGLTGKNRQELLNRVKNGDVDIVVGTVALLEEDVAFANLGLV
                     IIDEQHKFGVHQRAGLRKGHWPHCLVMTATPIPRTLAMTAFGDLDVSMIKHTPPGRGE
                     VITRWVQPPNRRDAMEFIRQRLRAKKQAYFVYPRIDAFSEADSANGDIKAAVDEYHYL
                     KEKIFPEFKIGLLHGQMPSAEKDQVMEQFRTGKLDVLVSTVVIEVGVDVPNATIMVIE
                     NANRFGLAQLHQLRGRIGRGQSKSYCLLFAETDDEKAVSRLQILTRSRDGFEIAEQDL
                     RLRGPGELFSARQHGLPDLKIANIVDDFDLLNLARRDAFELIEADPALTSPHNAAVRQ
                     ELIRRFGAKLGLADIG"
     gene            complement(9476..12004)
                     /locus_tag="PLE88_08595"
     CDS             complement(9476..12004)
                     /locus_tag="PLE88_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOM76558.1"
                     /translation="MKHANVYRIAGEFILLCICIFLFVSTLSFDIGDSPSEYVWPYNN
                     PPHNLCGRAGAFAAYYALYYFGPGILVAWFSAAAALAIHICGKSVTQPLLRLVGLILV
                     VASISTAWYMVWPYETLGLFKNGSFPTGNGGILGVGAAVYLNQHLAKLGSWLVVLCCW
                     TVGALLLADSVVFALVRWFGTVVLKAMGLAEPAWNAACEHSQALGDIWIRLSQRQKKQ
                     AASFAQAVHNYTAQHKAKSLEQQPLISESAGSVPQEIQPVLSQPAAQEPPDISVKHAE
                     TSGSSEPEEKKQAVAQKPKQSVRPKTTLPVQRSYDDYQLPPLELLREPETGFAAVQSK
                     MVEHKAKALEDLLQEFGISANVVNAEAGPAITMYELELAPGVKVNQISALSNDMARAL
                     GSGSVRVVAPLAGKHTIGIEVPNSQREIVRLKELIQKGTAAAKMQIPLFLGKSSSGEV
                     LVSDLASMPHLLIAGTTGSGKSVCINSIITSILLTRRPDEVKLILIDPKMVEMAMFES
                     IPHLMCPTITEMRRAEQILEWAMQKMDERYELLAEAQVRNIASYNLLTAEQLIERFKP
                     SSQEEEAKIPKKLPHIVIIIDELADLMMTSAKEVEAYIVRIAQKSRAVGIHLVLATQR
                     PQATVVTGLIKSNMPSRIAFRVAARMDSRIILDQNGAEALLGQGDMLFLRPGTSELIR
                     SQGAYLEDDEIRGIVEFLKNVAQPQYSTELMQLNTVDLSAAKQDELFEDAVKVVLETQ
                     RGSVSLLQRRLGIGYARASRIIETMAAMGLLGEYKGSQAREVVMTLEEYEAMRKNMIH
                     DQVAGYSDLTQEADPSDEIESNPDDDFAHEEHILTPQEENIDTR"
     gene            complement(12221..12730)
                     /locus_tag="PLE88_08600"
     CDS             complement(12221..12730)
                     /locus_tag="PLE88_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76559.1"
                     /translation="MDSENMLRLARIIRFFLFAVFFLIGMIAMVLSILAEPELVQYYQ
                     SRNRLLEIQQQNARMRSLSSKYDAQIAFIEAEPNILERLSPVTFGRKPSAPDTDFPEV
                     RNRALREETEKLIAQIESKPQQDPIPAWLERILNPSNRRALFFAGAGLVLITFIFFGT
                     SGGQLIVEC"
     gene            complement(12865..14154)
                     /gene="eno"
                     /locus_tag="PLE88_08605"
     CDS             complement(12865..14154)
                     /gene="eno"
                     /locus_tag="PLE88_08605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOM76560.1"
                     /translation="MYTTIVDVKAREILDSRGNPTVEVDVLLDDGSFGRAAVPSGAST
                     GVHEACELRDAKSKRYNGKGVLSAVKNVNEKIAPEILGMDAMAQEELDAFLCKLDGTA
                     NKSKLGANAILGVSLAVAKAAAISVDLPLYRYIGGCNANILPVPMMNILNGGKHADNN
                     VDFQEFMVMPVGAKDFPTALQMGVEVFHSLKSVLKSKGYNTAVGDEGGFAPSLKSNQE
                     AIEVILEAIKKAGYKAGKDIFIALDPAASEMWTGSTKKYKFFKSDPKKQITGKQMADY
                     WMEWLNKYPIVSLEDGLAEDDWDSWKYLTEKVGGRYQLVGDDLFVTNTKRLAAGIEKG
                     CANSILIKVNQIGTLTETLEAINLAQRNGYTAVISHRSGETEDATIADLAVATGVGQI
                     KTGSACRTDRICKYNQLLRIHEELGSAARYGGEIFKY"
     gene            complement(14297..15304)
                     /locus_tag="PLE88_08610"
     CDS             complement(14297..15304)
                     /locus_tag="PLE88_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOM76561.1"
                     /translation="MMYNPAKPLLVLLCIVSAVSGYAAENMALEDFRAHDPFILADSG
                     SQTYYLYTSITSGALPRGQAGVVAYTSKDLKLWQGPKVVFQVPQDGWANPAHGAWAPE
                     VHLYNGKYYLFVTLHNRDKIYTQPPQTPRVTHMRATQIFVSATPDGPFVPLADRPATP
                     DGLMTLDGTLFVEDGIPWIVYCHEWIQVTNGTVEAVRLKPDLSGPQEDKPLMLFEGSD
                     APWITPWQAKPDDESRTFVTDGCFLWRTKTGRLLMLWSSWQENKKYAQTVAFSLSGRL
                     AGPWRQLEPLLTDDSGHGMVFRTFDGRLMLVCHQPTMSPQSRARLHELEDTGDSLRLK
                     E"
     gene            complement(15301..>15765)
                     /locus_tag="PLE88_08615"
     CDS             complement(15301..>15765)
                     /locus_tag="PLE88_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76562.1"
                     /translation="CFRLQGQTCETLEKITDPKRFGYWFVPRFREFADRENLLPFDQH
                     LLRALVAPRGLLSTDSVDDLWANPYGTQQSYLAAQPVFEWLGVPENQGIWFRRGPHDQ
                     NEEDWQALVDFADMLFFGKAPQQERRFDQLAFEPDQSLVQNPFKKPSARGKK"
BASE COUNT         4113 a         4137 c         3692 g         3823 t
ORIGIN      
        1 accagattat ctgcggagcc gattccctca ataacatcat cgaagatact gtggtagccg
       61 caatggagcc gcaaaatgtt ctccagcata ttcacgacaa ctccattata attattcccg
      121 gcgaccgcgt agataatatt ctggtctcca ttctcgtgct gtcccgcgga caacccagcg
      181 gcggcattat tctcaccggc ggttttgaac cccatccgat gattgaaccc ctgcttaaaa
      241 gcagccgcat ccctgttctg ctcagtcagg aagacacatt taccgtcagt gctcgaatga
      301 aggatcttgg cttcaaaatt cgctcttatg atacagaaaa ggtagctcgt cttcatgatt
      361 tagtagataa gtatgtagat tgcggcttta ttctaaatgc cgttcagcaa tagcagcgtt
      421 gcaggccggc tatatctttt gaagctttat tacaaaataa caagcttttg ttcacctata
      481 ttgtccattg tgccgcattg ttccccttag ggttgaattc tcatacaaat aggtagagat
      541 ttatccaact acccgccttt tggaaatttt aaacctcctc cttattgcca gcgtataata
      601 agagaagtat ttttaatgga ggtcagaata atgcataccc atattcacag acctgaatgg
      661 aacaaaataa aacttcagat ggacgatatt cttcgggatc cacgtttctg ggctgtgctg
      721 gtcctgattg tgctattcgg attaatggtt ctgacggcat tgtttacaaa gcagggaagc
      781 ggtacgatac cggtccatcc tttttatatg ccatagggct gccaagagga ctcggccgaa
      841 gttggcagcc tgtattataa atccggcaaa gaagaggtat attttctgaa aggagaagca
      901 atgagcgtac aagaatatct tcagcagcat cattgcgggt acagggtaac agaacataaa
      961 ccagttttta cagccgaaca attggcggtc gttgagcatg tatcgcctcg gcaggtggca
     1021 aaaccagttg tcgtgcgatg tgatgggcag tattacttat gcgtcctgcc cgcagacaga
     1081 aaaatagact tttatgccat tgggcatcat ttaaaggtta aaaatgtcga tctggccagc
     1141 gaacaggaga tgaaaacgct ttttactgac agtgaactgg gggctgaacc gcccttcggc
     1201 aatttatata accttccaac gctgatggac aagacactgg ccaaagacaa ggaaatcgtt
     1261 tttcaggcag gcagccacaa gcagtcgctt tggatgagca tggacgacta taaaaagctg
     1321 actaatccgg atatcttttc tttcagctac ccacaagtac tcgatgagat tgaatcgatg
     1381 ccgtttgacc ccttttttta tgacccgtac ggcatctaaa aatgataaaa tttcagccgt
     1441 ttgaagcagt gtattcttcc aataaaaaat agaactgtgc aggggctgga ttttagccgc
     1501 ttatgccaaa tcgggattag gggtcaacga taatttctta ataaacgtgt aatttttcat
     1561 tccactgata agttttgaaa agtttgcctg ccttcttttg tttttgcttg accatccgca
     1621 gaaactttct tacagtacaa ccaacaacag cataatgaaa aggaaaatat aaactaagct
     1681 gggtaggttc gtacatggct gacgagcagg ttttagtaat caagcggtca atcatcgaag
     1741 aaatcggggt atttcacgga ttgatgttta acatccggga gtacttggac cgtatttttg
     1801 ccgccggggc aggacagttt attccccgca cgcaggcgga aaaagatccc ggtttcaagc
     1861 agctgattcc gtatgttctc atcagctgca gcggacggta cctgacctat gtccgcggca
     1921 agcgggccgg cgaaagccgc ctgatagcca agcgttccat cggcattgga ggacacatca
     1981 atcccaccga tgatatgtcg ctcttttccc cgtaccagac atatctcaat gccgtccagc
     2041 gggaggtcga agaggaaatt tacattcaag cacgctatac cgaccgtatt gccgccctgc
     2101 tcaatgacga caccaacgaa gtcggcgctg tgcatttggg cattgtacac ctctggacat
     2161 tagactccca gcaagtcagc cgaaaagagc aaatgattac ccagcttgaa tttatgaaca
     2221 aagatgcact gcggcaggtt cgggaacaga tggaaacatg gtcgcagctg tgtctggatg
     2281 agctggacaa tatcgaaaaa accgtccttt cctgaatgcc gttcagctgt tcctcaggca
     2341 gatgaatgtt atctggtttc ggctgccaag ccgcatgggt ggcccccacg tgccggagcc
     2401 cgttgaaata tacagatgca ttcggcctat cctatgcaat cctttgaggt attgaaaacg
     2461 gctgcgtacc acccaattga acggccagat ttggccgttg tgcgtgtgtc cacacagcat
     2521 caagtcaatc cctgctctgg ctgcagcgtt gtatccttcg ggctggtgat aaagcaaaac
     2581 cgtaaacaga tccggctgtc gctgaagacg cgttaacata tgctcaaccc atgccggatc
     2641 atcgctgtcg tcaatgccga caagctgtat gccgctccat tttaccgctt cattccgcag
     2701 cggaataacc gctgtctgct tcagcagcgc agcagctttt tccagtccga aatagcgttc
     2761 gtggttgcct gatatccaat agatcgggac attcaaacgc tgcagaggca agagcgcatc
     2821 cggcggcaaa tgcccttgcg gatcaataag gtcgcctgta atgacaacag catccggctt
     2881 cagagctgcc gatttttcaa caagccgttc caaatgccgc cagctgactg aacccagatg
     2941 aatatcgctt aactgcacaa tattcaggtt tacgggcgct ggcagctcaa tggtttcgac
     3001 attaatgcat ctggcattca taaaagcata caccgacaga agaagaatcg tccctgcggc
     3061 aaaccatcgg gaaatgtcca atggaaatcg aaaaatcaga tacagcaaat catgactgag
     3121 cagaacaatc aagcacagcc agccgacccc caaaccgatt gctgaaagcc gtacaaaaaa
     3181 attgcatagg ccgcacggat atttgcgttc caggatggaa aaaaggatat acccaaacgc
     3241 cagccagacc agcatcagcc agaaaaacat agtgtgcttg atcttaaaga atgcgaacag
     3301 ccgcgacagg atataataat atccgcctaa gcaaattcct gagaaaatgg atataaaaac
     3361 cagcacccca aacctttgca ttataaaacc agtccgactg tcaagctggg ttattcctgc
     3421 attctcatct gccgtttttg acagattcga aggaatttcc tatttccacc tgaaccttcc
     3481 cgttttggat tagtactatt gtagggacat tttgttcgat tcatacaaga aaaggatata
     3541 aaataccgaa taacaacggt gattttgacc attcaagaaa tcgtgatttg gggaaaacta
     3601 tgaacctgaa tgaatatttt gaaaatacag atggcgttgg aatcttggca acggccgaca
     3661 gcaaagggaa tgtggactgt gcgatttatg ccacaccgca tatcatcgat gaggaaacaa
     3721 tcgcatttat tatgcggccg cgtctgagtt ataacaatat ccggcagaac ccgaaagccg
     3781 tttatatgtt tattgaaaaa ggagccggct ataaagggcg tcggctatat ctggaaatga
     3841 gccatgttga gaccaattct gaaaaggttc atctgattcg tcggaaatcc cacggcggag
     3901 gcgacgaggc agatgccaaa ctggtttatt tcaaagtgat gctgacacga ccgctggttg
     3961 gggaacaaga gacatcctga tatgaaccgt cagcttctcc tactatgcac aatttgcatt
     4021 ctgatatgcc ttgcggtggg aggaatcagc ggattgaccg tacagaatgg cggcggagaa
     4081 tggtatgcag ctttaaagcg tccgccgctg aatccgccgg cacaggtatt cgggattgta
     4141 tggcccatac tgtatgttct aatgggcatt gcagcaggtc taatctgggc ccgtggctgg
     4201 caaactgccg gcgtaaagca ggccttgttg gtctttggta ttcagcttgt gctcaacggg
     4261 ctgtggagtc cgctgttttt tggtctgcat tggataggag cagcattggc agagtgtatt
     4321 ttgctgtggg caatggtttt agctgccgca gcaagattta aaaccatttg tccatccgct
     4381 tcccttttgc tctggccgta tctgggctgg ctgacatttg ctgtgtatct gaatgccggc
     4441 tactggattc ttaatagata acacagcctc ctataaaatc tgcctgctaa gatagggtga
     4501 taacaaaaag cagaaattga atagcgtatt tattcttgag gcagtcgggc taaatctgca
     4561 tcggctccgg cgaccataag aatatcatcc tgatagactt tggtttccgg cataggaaca
     4621 ttgagaattt cgccggattc ctcctgttca acagccctgc ttcgcttaat tgctacaagg
     4681 ttaagattgt atttttgccg aagctgcagt tctcgaacag tcttgccgtc aaaactggcc
     4741 ggagcagaaa cccgggccag actgtacccc ggggaaaaat cgattttttc ctcaatatgc
     4801 ggtgtaatca gccggtatgc ccaccgctgg gctgtttcta tttcgggata tatcacctcg
     4861 gttgccccga tggccgtaaa aacagttccg gcagttgtgt tttccgcacg ggcaaaaatc
     4921 tgcggaatcc ccaatttttt gagattcaca accgttaata tagccgattc aaatccgtgc
     4981 ccggctccca ttccgacaac cgcgatatcg accctgtcga ttccctgagc ttttagggca
     5041 tcgatatcgg tgctgtcgag ccgaatagcc actgcaacct ggtcacgtat gttttcaatc
     5101 acctctcggt ctctgtcaat tgcaatcact tcagctccgc cggcagacaa ggcacgtgcc
     5161 aaactgctgc caaaccgccc taatccaatc actacaaaac gcttcatatt caatcctcat
     5221 tataaaagaa agtccggctg ccaagccgac tactataagg ttcctgtttg attatccttt
     5281 atccaacaac cagcggctct accggataat catatttggc cggcttgaca ttaaacataa
     5341 gcgctgtcag cagcgtcatc ggccccagac gtccaatgag catcatggca acaataatcc
     5401 atttgcccgc cgtggtcaaa accggcgtaa ttccagtcgt taatcccaca gtacccatcg
     5461 ccgaggccgc ttcaaaagcc acatcttcaa tcggcaggga ttcgccacgt tctgtaattg
     5521 tcagcgccag tgtgccggcc agcagcataa ccgcatagag aaccgtaatt gttaccgccc
     5581 gtccgagcac taccagcgga acagcccgac gaaatatttc cacttctctg cgcttcccca
     5641 aagttgcata aatcaccatt actaccaaag caaaggtaac cgtttttatg ccgccggccg
     5701 tagaaccggg cgagccgccg atgcacatca gaataataag aatcagctta ctggccggag
     5761 aaagcgccgc aatatcaatg gtattgaagc ccgctgttct ggcggtaata gattgaaaca
     5821 gggctgacaa aaaccgggga tcccgttgag gactggtcga acaggcctca aacagcatca
     5881 aacctgcggt accggccata atcaaaagaa gggtcgtggt tagaaccact ttcgtctgaa
     5941 gcgtcagccg ccgtggactt tggagattaa atacatccgg atagatgccc tttttgctga
     6001 aaaggcgatt tagccgctgg ccggagacct ctgccagatt ataaagaacg gcaaatccga
     6061 gaccgccgag aataatcagg ggacagataa ctgcatagac agagatgcaa gaacggaaat
     6121 ccatcagact gttgctcatc agcgagaaac cggcattgca aaaggcactg atggaatgaa
     6181 aaatactgaa aaaccagcga cgctccggag ctaccagact tggcacataa tcccacattg
     6241 gatacaggca gactgccccg acggcctcaa caagaagcgt tgaaacaata atgaagccga
     6301 ccattgtgct gatgcggcta agcgtgcgtt cgttaagtaa atcctgcaga gccgcctgtt
     6361 cccgcattgt catggattga cccagaagca gccctaaaac agccccaaag atcacaatgc
     6421 ccaatccacc cagctgcatc agcgacaaaa taacaatctg accaaagagt gtaaaatcac
     6481 ggccggtatt tttaacaatc agccctgtta cgcacgtggc gctggttgcc gtgaaaaccg
     6541 catccgtcac atttattgtt ccctcatgcg cccggggcag catcagcagg ccggcccctg
     6601 ccagaatcag caccacaaac agggtaatca gcgaacgggc aggattgtgc cctgtggagg
     6661 ccagatagac ggtgcttcgg cagactttgt cgatcacctg tactatcagg taaatgctga
     6721 tgccttgaac aaaccactca aagatttgac cggaggacca cttttctgcc cccaaaaaaa
     6781 gaacaaacag caaacccagc agaggaattt cgaaccagta cagccggaaa aaatcccgct
     6841 tggaaactgc gttaaaatac cgaatcaatt tttctgttac aaaaaaacag aacaggatga
     6901 tttcggcact gtgcagccag atgctgggca aaggcggctg gtcaaaacca tacagcatca
     6961 caaaagtcga tgctacagcc gcaattccga taagattgag gccaacaata accgcatcca
     7021 gctttttatt cttgtattcg actttcatcc atcaagtcag aaaacattct gcctttatgt
     7081 ctgattggat atgttcaata tccggtatta taagatttcc tgatttgttt tacccgcttt
     7141 agggaaaaat ttccattctt ttttacccga tatcggccag accaagtttg gctccaaacc
     7201 gtcggatgag ttcctgacga acagccgcat tgtgaggact ggttaaagcc ggatccgctt
     7261 caatcagctc aaaggcatcc cgacgggcca gattcagcag atcaaaatca tctacaatat
     7321 tggcaatctt caaatccggc agaccatgct ggcgtgcact gaacaattca ccgggaccgc
     7381 gcagccgcag gtcctgttcg gcaatttcaa aaccatcccg gctgcgggtt aaaatctgca
     7441 gccggctgac agccttttca tcatccgttt cggcaaataa aagacaatag gacttgctct
     7501 ggccgcggcc gattcgtccg cgaagctggt gcagctgggc aaggccaaaa cgattggcat
     7561 tttctataac cataatggtt gcattgggaa catctactcc gacttcgatt accactgttg
     7621 aaaccagcac atccagcttg ccggtacgaa actgctccat cacctggtct ttttctgccg
     7681 acggcatctg gccgtgcaaa agcccaatct taaactccgg aaaaatcttt tctttcaaat
     7741 aatgatactc atcaaccgcg gcttttatgt cgccattggc gctgtcagcc tccgaaaaag
     7801 cgtcaatcct cggatacaca aaataggcct gctttttagc ccgcagccgc tgccgtataa
     7861 actccattgc atcgcgccga ttgggcggct gcacccagcg tgtaatcact tcgccccgtc
     7921 ccggcggagt gtgcttaatc atcgacacat ccaaatcgcc gaaggccgtc atcgccagcg
     7981 tgcgcgggat gggtgtggcc gtcatcacca agcaatgggg ccaatgacct ttgcgcaggc
     8041 cggcccgctg atgcactccg aatttgtgct gctcatcaat aataaccaga cccagattgg
     8101 caaaggccac atcttcttcc agcaaagcaa ccgtgccgac aacaatatcc acatctccat
     8161 ttttcacccg gttcaataac tcctgtctgt ttttgccggt cagtccaccg atgagcagtt
     8221 cccgccgaac ccggctgcct gccaaaaagc gttcgatgct ggcgaaatgc tggcgagcca
     8281 gaatttccgt cggggccata atggctgcct gtgttttgtt ggctactgca accagtgccg
     8341 catagagggc aaccaccgtc ttgcccgaac cgacatcacc ctgcagaagc cggttcatcg
     8401 ggacctcttt ggccatatca gcggtaatct cacggatgac ttgctcctga tcagctgtca
     8461 gcagaaatgg aaatcgctga cgaatgcggc ggtcaatttc atcggtgcac accattggca
     8521 ccgccggaca agcatgctga atatggtacc gtttcagcgc caaaccggcc tgcatgaaga
     8581 acagttcttc gtatttaagc ctgcgtctgg ctgcagcaag catatcctcg ttgtctggaa
     8641 tatgaatctg ccgataggcg tccggccggg gcatcaattc atttcttttc cgaaaagctg
     8701 catcgaacag ttccggcatc agaggagcca gcaagtcgat attcgtcctt acaatttttt
     8761 taatctgcca gctcggcagt ttggcgctgg ccggataaac cggaccgctg aatgcatccg
     8821 gcccgcgcgg ctgatcggga tggacaatca aaaacttcgg attattgatt tgaagctgat
     8881 atcggtagcg aatggccttg ccatgaacca gcagcatctg ccccggttcc agctggttaa
     8941 ccagatatcg gccgtgaaac cacacaatcc gaatagtccc tgtctggtcg gccagcgtcg
     9001 cctcaaaaag gggaattttc cggtacggct gccagtccgt ctgttccacc agcccgagaa
     9061 tacacactgt ttggttggca cgcacatcgg aaatggccat cggttcgggc ataaagaccc
     9121 aatcccgagg gtaatattcc agcaggtcac cgactgtttt cactcccagt tcatcaaacg
     9181 cttgtgcccg cataggcccc actccggcca gaaactgcac gggtgtgtct aatgttatcg
     9241 taccggcttg ctgcaccata tgcgttatct tatccgtcct tttgtcatta tgcaagtgcc
     9301 gcatagcaga gaaataaagt cccaaaatgc attctctgag attattttgc cgcaccgcca
     9361 gcacaggtcc ggctgtgaat ccggcagtcc tgatttatcc gtccgccggc gtcttgcaga
     9421 aatggccggt ttggtatgaa gcttacgttt ttattgaagg gcaataaaac agccgctacc
     9481 gggtatcaat attctcttcc tgaggcgtca ggatatgttc ttcgtgggca aaatcatcgt
     9541 caggattgct ttcgatttca tccgacggat cggcttcctg cgtcaaatcg ctgtaccctg
     9601 caacctgatc atgaatcata ttctttctca tcgcctcata ctcttcaaga gtcattacaa
     9661 cttcgcgggc ctggctgccc ttgtactcgc ccagcagtcc catcgccgcc atcgtctcaa
     9721 taatccgaga agcccgtgca tagccgattc ccaaccgccg ctgcagaagc gacacgctgc
     9781 cccgctgggt ctccaggaca actttgacag catcttcaaa gagctcatcc tgcttagcgg
     9841 ctgacaaatc tacggtattg agctgcatca actctgtact gtactgcggc tgggcaacat
     9901 tcttgaggaa ttccacaatc ccgcgaattt catcatcttc caaatatgcc ccctgcgacc
     9961 gtatcagctc gctggttccc ggacgcagaa acagcatatc cccctgcccc agcagcgctt
    10021 cagcgccgtt ttggtcaaga atgatccggc tgtccattct tgcggccacg cggaatgcaa
    10081 tccgcgaggg catattggat ttaataagcc ctgttaccac agtggcctga ggccgctggg
    10141 tggccaacac cagatgaatg ccgacagcac ggctcttctg cgctatgcgg acgatatagg
    10201 cctctacttc tttggcggat gtcatcatca aatcggccag ctcatcaatg ataataacaa
    10261 tatgcggcag cttcttagga attttggctt cctcttcctg actggagggc ttaaaccgtt
    10321 caataagctg ctcggctgtc agcagattgt acgaggcgat attccgcacc tgcgcttctg
    10381 ccagcagctc gtaacgctcg tccattttct gcatggccca ttcgagaatc tgctcggcac
    10441 gccgcatctc cgtaattgtc ggacacatca gatgcggaat cgactcgaac attgccattt
    10501 ccaccatttt agggtcaatc agaataagct tgacttcatc cggccggcgg gtcaaaagga
    10561 tgctggtaat gatgctgttg atgcagacgc tcttgcccga accggtcgta ccggctatca
    10621 gcagatgggg catggaggcc aagtctgaca ccagcacctc cccggaggag cttttgccaa
    10681 gaaacagcgg aatctgcatt ttagcagctg ctgttccctt ctgaatcaat tccttcagcc
    10741 gaacaatttc ccgctggctg ttgggaacct cgatgccaat tgtatgcttg ccggccagcg
    10801 gtgctacaac ccgcacagag ccggaaccca gtgcacgagc catatcattc gacagcgccg
    10861 aaatctggtt gaccttgacc ccgggagcca gctccagctc atacatcgta attgccggtc
    10921 ctgcttccgc attcaccaca ttggcgctga tgccgaactc ctgaagcagg tcctcaaggg
    10981 ctttggcttt gtgctcgacc attttgctct gaacagcagc aaaacccgtc tccggctccc
    11041 gcagcagttc cagcggcgga agctgataat catcataact tctctgaacc ggcaaagtgg
    11101 ttttaggacg cacactttgc ttgggcttct gcgccaccgc ctgttttttc tcttccggtt
    11161 cactggatcc gctggtttcg gcatgtttta cagatatatc cggcggttcc tgtgccgccg
    11221 gctgggaaag aacaggctgg atttcttgcg gaacagatcc ggcggattcg gaaatcagcg
    11281 gctgctgttc taaggatttg gctttatgct gggcagtata attgtgaaca gcctgcgcaa
    11341 atgaggcggc ctgttttttc tgacgctgac tgagccggat ccatatatcg cccaatgcct
    11401 gcgaatgctc acaagccgca ttccaggccg gctcagccag tcccatcgcc ttcaacacga
    11461 ctgtgccaaa ccagcggacc aatgcaaaaa ccaccgagtc agccagcagc agcgctccca
    11521 ctgtccagca gcacaggaca acaagccagc tgcctaattt ggcaagatgc tgattcagat
    11581 acactgccgc acctacaccc aagatgccac cgtttccagt cgggaaagac ccattcttaa
    11641 acagccccaa tgtctcatag ggccaaacca tataccatgc tgttgaaata gaagccacta
    11701 ccaaaatcag tccgaccaga cgcaggagcg gctgggtaac agatttgccg cagatatgta
    11761 ttgccaacgc cgccgccgcc gaaaaccaag ccaccagaat gcccggcccg aaataataca
    11821 aagcataata ggccgcaaaa gcacccgcac gaccgcagag attgtgcggg ggattattgt
    11881 acggccaaac atactcactg ggcgaatcgc ctatatcaaa acttaaggta ctgacgaata
    11941 agaaaataca gatgcacagg aggataaatt ctccagcaat ccgatagaca tttgcgtgct
    12001 tcacggttgt ttataatcct tgtttttatt attaaaaaca cctattaaaa atcttgcttt
    12061 gtattttata tcggcatttg ggactgattt tatgcaaaag ctgtctggca atgaataaaa
    12121 atatcttcat ctgttatcaa aactgtgatt tcaacattaa aaacaggtaa aatagaaaag
    12181 aaagttaaaa aatgcaattt gatcgaggat ctggaagaag tcagcattcc acaatcagct
    12241 gaccgccaga ggtgccaaaa aagatgaaag taatcagcac aaggccggct cctgcaaaaa
    12301 ataaggcccg ccgattgctg ggattgagaa tccgctcaag ccaagcaggt atgggatcct
    12361 gttggggttt gctttcaatc tgggcaatca atttttcagt ttcttctctt aacgccctat
    12421 tgcgaacttc gggaaaatcg gtatccggtg ctgacggctt gcggccgaat gttacaggac
    12481 taagacgctc caatatattc ggttctgctt ctatgaatgc aatctgggca tcatatttgc
    12541 tgcttaacga gcgcattctc gcattctgct gctggatttc cagcaatcga ttgcggcttt
    12601 gataatattg taccagctcc ggctcggcta aaattgacag aaccattgca atcattccaa
    12661 tcaggaaaaa gactgcaaat aaaaagaacc ggattatccg tgcaagccgc agcatatttt
    12721 cactatccaa acctgaaaca catccccgca tcagcgttgg catctgtagc gaaaggggcg
    12781 tttcaaattc gataaataag atcagaaaaa aagggcacag acgcaagaaa tgcccctgtg
    12841 cccattttat cggtctttaa agggttagta cttgaagatt tccccgccgt aacgggctgc
    12901 cgagccgagt tcctcatgaa tccgcagaag ctggttgtat ttgcagatgc gatctgtacg
    12961 gcaggcagaa cctgtcttaa tttgtcccac acctgtggct actgccaaat cggctatggt
    13021 ggcgtcttcg gtttcgccgg aacgatggct tatgacggca gtataaccat tgcgctgagc
    13081 caaattaatg gcttccaagg tctcggtaag cgtgccgatc tggttgacct tgataagaat
    13141 agaattggca cagccctttt ctattccggc agccagtcgc ttggtattgg taacaaacag
    13201 gtcatcgccg acaagctgat atcggccgcc gactttttca gtcaggtatt tccagctgtc
    13261 ccagtcatct tcagccagac catcttccag actgacgata ggatatttat tcagccattc
    13321 catccagtag tcagccatct gcttgccggt aatctgtttc ttcggatcgg atttgaagaa
    13381 cttgtatttc ttggtgctgc cggtccacat ttcagaggct gccggatcca gcgcaatgaa
    13441 aatatccttg cccgctttat agccggcctt cttgatggct tcgagaataa cctcgatggc
    13501 ttcctgattg ctcttaagag aaggagcaaa accgccttcg tcgccgacag ctgtattata
    13561 gcccttgctt ttcaacacac ttttgagcga atggaagacc tcgacaccca tctgcaaagc
    13621 agtcgggaaa tctttggcgc cgacaggcat caccataaat tcctgaaagt ccacattgtt
    13681 gtcggcgtgt ttgccgccgt tgagaatgtt catcatcggc accggcagga tattggcatt
    13741 gcatccaccg atgtagcgat acagcggcaa atccacagag attgccgccg ctttagcaac
    13801 cgccaatgag acacctaaaa tagcattggc accgagcttg gacttgttag cagttccatc
    13861 cagcttgcat agaaatgcat ccagctcttc ctgagccata gcatccatac cgagtatttc
    13921 gggagcgatt ttttcattta cattcttaac agccgacaaa acgcctttgc cgttatagcg
    13981 tttggacttg gcatcacgca gctcgcaggc ctcatggaca cccgtactgg caccgctggg
    14041 cactgccgca cgtccaaagg aaccatcgtc cagaagaaca tccacctcga cggttggatt
    14101 gccgcgcgag tccagaattt ctcgtgcttt tacatcaaca attgttgtgt acattggtct
    14161 ttcctttcat tcttaaagca gttgctttta ctgtatccta ttattcagca ggccttgccc
    14221 gaagaaacgg cataaaaagg tacaccacag ccgccgcttc gtcaagaatg gattttgcga
    14281 aaaagagcaa ttgcctctat tccttaagcc gcagactgtc gccagtatct tccaattcgt
    14341 gaagacgggc acgggattgc gggctcatgg ttggctggtg gcataccagc atcagccggc
    14401 cgtcaaatgt ccgaaagacc atgccatgcc cgctgtcatc agtcagcagg ggctcaagct
    14461 gccgccatgg accagccagc cggcccgaca atgaaaaagc aaccgtttgg gcatatttct
    14521 tgttttcctg ccagcttgac cagagcatca gcagccgtcc ggttttggtc cgccaaagaa
    14581 aacagccgtc ggtaacaaac gtacgggatt catcgtccgg tttggcctgc caaggggtaa
    14641 tccatggcgc atcagatcct tcaaagagca tcaggggttt atcctcttgg ggaccggata
    14701 gatccggttt gagacgtacg gcctcaacag tgccgttggt aacctgtatc cactcatggc
    14761 agtaaactat ccacggaatg ccgtcttcaa caaaaagcgt gccgtccagt gtcatcaggc
    14821 cgtcgggtgt tgccggccgg tccgccagcg gcacaaatgg cccgtcgggt gtagcgctga
    14881 caaaaatctg cgtagcccgc atatgggtaa cacgcggtgt ttgcggcggc tgggtataga
    14941 ttttgtcgcg gttatgaaga gtaacaaaca agtaatattt gccattgtat aaatgtacct
    15001 cgggcgccca ggcaccatga gcaggattag cccagccgtc ttggggtact tgaaatacga
    15061 ctttgggacc ctgccacagc ttgagatcct tgcttgtgta agcgaccaca ccggcctggc
    15121 cccgcggcag agcaccggag gtgatgctgg tgtagagata ataggtttgg ctgccggaat
    15181 cagccaaaat aaatggatca tgggcacgaa aatcttccag agccatattt tccgctgcat
    15241 acccactaac agccgaaacg atgcataata agacaagaag cggcttagca ggattataca
    15301 tcattttttc ccccttgctg atggtttctt aaaaggattt tgaacaagtg attggtccgg
    15361 ctcaaatgca agctggtcaa accgtcgttc ttgctggggc gcttttccga aaaagagcat
    15421 atcggcaaaa tccaccaatg cctgccagtc ttcttcattc tggtcgtgcg gccctctgcg
    15481 aaaccagatg ccctgatttt caggaacacc cagccattca aacaccggct gtgcggccag
    15541 atacgattgc tgagtgccgt aaggattggc ccacagatca tccactgaat cagtggacaa
    15601 aagcccgcgg ggggcaacca gtgcccgaag cagatgctgg tcaaaaggca atagattttc
    15661 tctgtctgca aactcccgaa atcgcggcac aaaccaatat ccaaaccgct tggggtcagt
    15721 aattttctcg agagtttcgc acgtctgtcc ctgcagacgg aaaca
//
LOCUS       DAOTBO010000068        15411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3214_length_15411_cov_4.803334, whole genome shotgun sequence.
ACCESSION   DAOTBO010000068 DAOTBO010000000
VERSION     DAOTBO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3214_length_15411_cov_4.803334"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /gene="acpS"
                     /locus_tag="PLE88_08620"
     CDS             <1..310
                     /gene="acpS"
                     /locus_tag="PLE88_08620"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOM76563.1"
                     /translation="VKNRIEKLAGRFAAKEAVMKLIGTGWRDGIAWTDIEIVNNAFGQ
                     PQVHLSGRVRQIAERSGIEQVTLSITHTANFAIASAVALQAVDGSAGTAPPQDKERIH
                     "
     gene            390..2849
                     /locus_tag="PLE88_08625"
     CDS             390..2849
                     /locus_tag="PLE88_08625"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOM76564.1"
                     /translation="MRNASSVSRRDLLKAILWTPPVLMFSKGGLFAQQEVRPLFAPRI
                     VPPGQKIRVAQIGVFNRGADILRSFSQYKDQLEYKAFSDVLFLTPSYQMRNFPGVPCF
                     RDYRQMLEQMHEQIDAVIIATPDHAHFPMAMHAMLLGKHVYVEKPMAQNVYECRLLEK
                     TARQCGVVTQMGNQGHSGAGTIQFGQWVEAGLVQNVQKIDAWMTNSRRWHGWTDTAYP
                     ESIPPVGYDWDLWLCRRPFRPYSEKLVNGNWRCWYEFGCGAMGDWGAHILDAVHRYLK
                     LGLPYEIATTLKGPSDLLYPQGSVITFKFKARGALPPLELRWYDGQRNNPTPPPGITG
                     DIGNVGSFIYAQDYVIRGTSHGANYKILPAEKMAQLEQAGKLPKPSRRLPDHYQNFLN
                     ACQGIEPANSSFDVAAPLAELLCLGCIGQRFGGTLLYDAEAMKITNHPEADQMLKGPA
                     VRDGWDAYDRRQLVKSKASQIQSPDSVPWEDLLADPALSSWENPYDWGKAEFKDGVVS
                     LTSENGKWFLLTKKEYANFVFEGEVKMPPNQGNSGFMFRCQKAKNRVWGYQAEVDTAD
                     RKWSGGLYDEGRRMWFASPNRDQADTEEEKNRSIAEFRARAGDCYKQGEWNKYRIVCI
                     GPHIQIYVNDILTTDIFDEMDLAGPVGIQHHGEKGLTYQFRNLRIKDLGAGGPIYYPH
                     REGAKAAAVPSKMDGAIYEAEEANVLSDAKAADNHKGYQGTGFADMGGKGSFIEWNNV
                     LADTAGSYTLTFRYAAAGSRPCDLTVNGANAGRLAFADTGSWTSWKTETIAVTFKNGG
                     NSVRLTAVGDGPNIDALAVTK"
     gene            complement(2912..3565)
                     /locus_tag="PLE88_08630"
     CDS             complement(2912..3565)
                     /locus_tag="PLE88_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOM76565.1"
                     /translation="MKKTAPSLRFLITAGGTREYIDPVRFISNASSGRMGYALARAAV
                     RAGHAVTLITAPTALAVPKGVRVRRVVSSDEMLAAVQAEFAACDCLIMAAAVSDYKPA
                     RFSRTKIKKEQAVLSLKLKPTVDILRWAGRNKQSGQFLVGFALEDRKLRQNAEKKLRS
                     KNLDMIVANTPSAIGADTSALCIKLGGGDWQVLPAGPKASSARRIIGLICRALLGKK"
     gene            complement(3567..5465)
                     /locus_tag="PLE88_08635"
     CDS             complement(3567..5465)
                     /locus_tag="PLE88_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOM76566.1"
                     /translation="MKQKIEMSAVRSINRFFAANYVLMLANLTGFVIAAPKADFGAAV
                     FIVSVMLAYALVYLIPAGLLLSAVRGVIGIGQLCRKGYAQLLMYAAAVLVMTLTHLVI
                     LIDGIIYRMYDFHINGFVWNLLTTKGGIESMGFEQGVYWTAGLFLLAALALEIFLLFL
                     CIRWEDWNISRASRWPHRLRVSVPFMAAGMFGLQALLFGISSFRGNAGILAASGAFPF
                     YMPMTFQGILKDLGFQPGRRELLTVHSSKGLALQYPLEPIRRRPDSPRWNIVWLVAES
                     WRADMLNPEVMPRTWEFARRAQYFTNHYSGGNGTCQAMFSMFYGLYGNYWFRFLNEQR
                     SPVLMDLLQDNGYQIDLYTSARFSYPEFDKTLFSRIPPEHLHDYSQGEGWERDRVNTG
                     RLLDFIDRRDKDKPFMTFLFFESPHARYYFPPECEIRKDYLKDFNYATADLQRDIGRI
                     FNRYVNSCRHLDTQFARIIDYLQQHQLLDSTVVILTGDHGEEFMEHGHWGHNSGFTEE
                     QVRTPLVLWVPGRPAAEYAKMTSHLDIPATLMTLLGVDNPPRDYSLGFDLFSPATRFY
                     TVLGDWDSLVYIDAECKIVLPLKATAVFNKITDADDTPAEDSAGLYSKKMANLVGLLR
                     EAGEFTTH"
     gene            complement(5558..6121)
                     /locus_tag="PLE88_08640"
     CDS             complement(5558..6121)
                     /locus_tag="PLE88_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOM76567.1"
                     /translation="MPEQSKQLEGRRILLGISGGIAAYKAADLASKLTAGGAVVRTIM
                     TENACRLIAPATLEAVTGQPVYTSLWHGQDDFKIGHIQIADWAEMVVVAPATANLIAK
                     AANGICDDLLTTTLCACWDKKVLLAPAMNSRMWANPIVQRNLSILTDLLKIHLVGPET
                     GRLACGTEGIGRMAEVPQIVDAIQKIF"
     gene            complement(6130..6360)
                     /locus_tag="PLE88_08645"
     CDS             complement(6130..6360)
                     /locus_tag="PLE88_08645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOM76568.1"
                     /translation="MIEELKDTEIINKIGGRFKLSALIQKRMLELMEGGRPLIENTEG
                     LTQMEIVVQEIKQGKIALDITGDEKKPKITPF"
     gene            complement(6357..6941)
                     /gene="gmk"
                     /locus_tag="PLE88_08650"
     CDS             complement(6357..6941)
                     /gene="gmk"
                     /locus_tag="PLE88_08650"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOM76569.1"
                     /translation="MTNAQAAGKLVVISGPSGVGKSTICRRIVEKRDAFLSVSATTRK
                     PGASEENGRDYWFVSRQEFERRIRAGEFLEYADVFGNYYGTPRPPVEKALAEGRTVIL
                     EIDVQGGLQVRQKCPEAVLIFILPPRPEDLQQRMRCRGRGEDEASRKRRLETAEKEIA
                     AARKHYDYFVVNADLDAAIQEVMDIIDGICKGNI"
     gene            complement(6975..7859)
                     /locus_tag="PLE88_08655"
     CDS             complement(6975..7859)
                     /locus_tag="PLE88_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOM76570.1"
                     /translation="MIYSMTGFGQACAEVDGVVYTVEIRSVNNRFFKAQFRLPDIAAF
                     LESDIERLHRDTIVRGTVSYWLRMKNISGRALFDIDENTLKTYIRRLKDILDEDDAHS
                     RIDLASLLSLPGIVQPAIPDEASIEKMRQVIVALTQEALAMLQKSRQEEGRALAADLL
                     DHLDRIAFHLDKIRQRVDIVVQEYHSRLKSRISQLLAEAQLKIDENLLAREVAIFAER
                     SDIAEECSRLSAHLGQFRECCQTGGPVGRRLDFMAQEMLREANTISSKSSDTQISQSV
                     IEIKCAIDRLKEQVQNVE"
     gene            complement(7875..8306)
                     /gene="secG"
                     /locus_tag="PLE88_08660"
     CDS             complement(7875..8306)
                     /gene="secG"
                     /locus_tag="PLE88_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOM76571.1"
                     /translation="MSCVLALSLMGNLIVGLFILVCLALILIVLIQKGRGGGIGAAFG
                     GGGAGSLLGTKTGDFLTWVTICLVALFLVLAVLMGKFMRPSGSLNLSAPLMPAAAPSE
                     EAASEAAAPAVPEAQTPADQPADSAAGAPAQPVQETPEPAN"
     gene            complement(8336..9091)
                     /gene="tpiA"
                     /locus_tag="PLE88_08665"
     CDS             complement(8336..9091)
                     /gene="tpiA"
                     /locus_tag="PLE88_08665"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOM76572.1"
                     /translation="MRKPFIAGNWKMNTTSTSAAALARGLSSQLAGIASVDVAVCPPF
                     VYLQSVAAALSASNIALGAQDVYFEGNGAFTGEISVEMLKDCSCTYVIIGHSERRHIL
                     GETDGLINKKIAAAIAGGLLPIFCVGELLQEREAGKTTAVVAEQVKKGLDGLCSERVQ
                     AVTIAYEPVWAIGTGRTATPAQAQEVHLMIRNLLAQLYGGDIAQSMRIQYGGSAKPSN
                     TAELMSQPDVDGLLVGGASLKVEDFAAMVKTVA"
     gene            complement(9130..10200)
                     /gene="pheA"
                     /locus_tag="PLE88_08670"
     CDS             complement(9130..10200)
                     /gene="pheA"
                     /locus_tag="PLE88_08670"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOM76573.1"
                     /translation="MSLEELRKKIDALDTKLVELINERARIVVEIGKLKQASESPVYM
                     PHREKEVLDRICEINKGPLPDKTLQAIWRELMSGSFFLERPLRIGYLGPRGSFSHNAA
                     ILKFGQSVDYEALADIRGIFEEVSKGHCDFGVVPIENSAGGGVRESLDAFIETDVMVC
                     AEVNMAIHHNLLANCPMREIARIYSQPEVFAQCRNWLAATFKDAQTIPVASSSKAAQL
                     AAEEAGSAAIASAIAAEIYGLKILCQDIEDISNNITRFLIISKQDAQRSGDDKTIILF
                     STAHRTGALVDVLNVFKDYDINMTNIGSRPNKKREWEYYFFVDFLGHKEDPNVKKALE
                     EAKSHCLQLSVLGSFPRSTTVL"
     gene            10371..10892
                     /locus_tag="PLE88_08675"
     CDS             10371..10892
                     /locus_tag="PLE88_08675"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOM76574.1"
                     /translation="MTQQQNYFYSIFKTHWGWFGLLGSDKGLIRTCLPTAFKEATQQR
                     ILSGIPAAVPAPKTFRQLEEQIISYYKGHPADFSSAAVCLEGLTEFQRHILTILRTIK
                     YGQTITYSELARLADCPRGARAIGAVMAVNPLPLIIPCHRVIKQDGSLGYFSAAGGVD
                     TKKRMLELEKAAI"
     gene            11040..11240
                     /locus_tag="PLE88_08680"
     CDS             11040..11240
                     /locus_tag="PLE88_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76575.1"
                     /translation="MTRAAKIAFISQFYIFGAGCLCPLLVIRSGTVPVMYLVGLFLVA
                     AVLFGIIWSVAVFCLYGRTTDA"
     gene            11233..11442
                     /locus_tag="PLE88_08685"
     CDS             11233..11442
                     /locus_tag="PLE88_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM76576.1"
                     /translation="MPDQRLSNQIRRLRFEAGEMTQQALADRVGVTRQTIIAIEQGRY
                     APSLPLAIKIARTFGKTVDEVFQLA"
     gene            complement(11494..14001)
                     /locus_tag="PLE88_08690"
     CDS             complement(11494..14001)
                     /locus_tag="PLE88_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOM76577.1"
                     /translation="MKRLLVVWLMGCLPAAAADMVPFVIPAGVHPDSAIRMQQAPLTE
                     QDRLHADGHFLTESGKRVRLWGVNFTFEANFPTHSDAREIARRLAAFGVNAVRCHHMD
                     TASWPRGIWDQTGRALHPEALDRLDYFIDQLAGQGIYVNLNLQVGRKHSEALGLPDPA
                     TEYNKMASLFTPQLIEAQKEYARLLLTHRNPYRSHVAYAQDYAVAIVEITNENSLFMW
                     DSEKSLRALPPFYADILRSQYSQWLKRKYGTTDRLRQVWDKQAQPLGPVNLLRASLPV
                     EGWRLEQHEQAKAAVSSPAAEAAGLTIDIANADGIAWHLQYNHPGLTFEQGQFYTVIL
                     KARAPRPRPLNVSVQQAHEPWSNLGLYRDIQLTPDWQTFTLGFTAAAGDANGRLNLAF
                     GMDGGTVQLGTVELRTGGQIGVAPGESLEEATVALFAESPVPARQIDQMCFLAETEKA
                     YFDQMRAFLKNDLNCRAMVTGTIVFGPLGLFAQSGMDFIDSHAYWQHPQFPRQPWDSG
                     DWLIEQKAMSGNPPGTLYELAAERLAGKPFTVTEYNHPAPLDSQAECVPMIASFAAAQ
                     DWDGVWIYTYSHSNNQFSRQHLNSYFDVDTNPAKWGFMPAGAVMYRQRALEPFAGKTA
                     VPLVSSDGSLLGALAELHLRIERDLSGPLRRLPAEKLDAFYHTFQPDGPVQTASGGSD
                     DAAVLEWTAQDGSGLYWMHNSRCEVFCGHRSRLDKDRHRLQIDQPDSAAVVITALDGL
                     PLSASGTILITACGRCENTGMQFSPDRRTVGRNWGTAPVRIEPVAGTLVLPLPAGQSA
                     TLKPLHPDGTVKDQIPIENGIVPLKAEYGTMWYLVQR"
     gene            14139..14429
                     /locus_tag="PLE88_08695"
     CDS             14139..14429
                     /locus_tag="PLE88_08695"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOM76578.1"
                     /translation="MAKDKTRQICIVTVTGKDKVGIIARISSVMAKANVNIVDVSQRI
                     MEDYFVMTMACDITDATADMDKLHRQLSRLGKELHLTITFQHENIFKMMHRV"
     gene            complement(14435..15136)
                     /locus_tag="PLE88_08700"
     CDS             complement(14435..15136)
                     /locus_tag="PLE88_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOM76579.1"
                     /translation="MSDFYVSAEETWRVFRIMAEFVEGFEELANIGPAVSIFGSARET
                     AGSRYYEMAAETARQLGKAGFAVITGGGGGIMEAANKGAREAGVKSVGLNIELPHEQI
                     PNKYQNLSLHFRYFFCRKVMFLKYAHGFVVLPGGFGTMDEFFESLVLIQTLKQAYFPV
                     ICMGSDYWGGLVDWIKTVMLGRHGYIAPEDMDVFTVCDEPQKAVQIIVDFHASNGRGG
                     IKQPWGIKRPLNGCQ"
BASE COUNT         3399 a         4731 c         4025 g         3256 t
ORIGIN      
        1 cgtcaagaac cggattgaaa aactggccgg acgctttgcg gccaaagaag ccgtaatgaa
       61 gctgatcggc accggctggc gcgacggaat cgcatggaca gatatcgaaa ttgtcaacaa
      121 cgccttcggc cagccgcagg tccatctgtc cggacgcgtc agacaaatcg cagaacgcag
      181 cggcattgag caggttacgc tcagtattac ccataccgcc aactttgcta tcgcctcggc
      241 agttgcttta caggcagttg acggctcagc cggcacggca ccgccgcaag acaaagaaag
      301 gatccattga aatattccct gcgccagctt cgttatccat caagcaggca ataatcgaaa
      361 caaatcataa gccaaaagga gtctaaaaga tgagaaatgc aagttctgta tcgcgccgcg
      421 acctgctgaa ggcgatttta tggacaccgc ctgtcctgat gttcagcaaa ggcggactgt
      481 tcgcccagca ggaagtgcgc cccctgtttg ccccccgcat cgtgccgccc ggccagaaaa
      541 tccgcgtcgc ccaaatcggc gtctttaacc gtggtgctga cattttgaga agcttcagcc
      601 agtacaaaga ccagctcgaa tacaaggcct tcagcgatgt cctgtttctg acgcccagct
      661 accagatgcg caattttccc ggagtgccgt gtttccggga ctaccgccag atgctcgagc
      721 agatgcacga gcagattgat gccgtcatca ttgccacgcc cgaccacgcc cactttccga
      781 tggcgatgca tgcgatgctc ttgggcaagc atgtgtatgt cgaaaagccg atggcccaga
      841 atgtgtacga gtgccgcctg ctggaaaaaa ccgcccgtca gtgcggcgtt gtaacccaga
      901 tgggcaatca gggccattcc ggagccggaa cgattcaatt cggccagtgg gtcgaagccg
      961 gactggttca aaatgtgcaa aaaatagatg cctggatgac caacagccgc cgctggcacg
     1021 gctggacgga tacggcgtat ccggagtcga ttccgccggt tggctacgac tgggatttgt
     1081 ggctgtgccg cagacctttc cgcccgtaca gcgaaaaact ggtcaacggc aactggcgct
     1141 gttggtatga attcggctgc ggcgcgatgg gcgactgggg ggcgcatatc ttggacgccg
     1201 tccaccgcta cctgaaactg gggctgccgt atgaaattgc cacaaccctc aaaggcccca
     1261 gcgacctgct gtacccgcag ggctctgtca tcaccttcaa attcaaagcc cgcggggctt
     1321 tgccgccgct ggagctgaga tggtacgatg gccagcgcaa caatcccacg cccccgccgg
     1381 gcatcaccgg cgatatcggc aatgtcggct cattcattta tgcccaagat tatgtcatcc
     1441 gcggcaccag tcacggagcc aattacaaga ttctgcctgc tgaaaaaatg gcccagctgg
     1501 agcaggccgg aaagctgccc aaacccagcc gaagactgcc ggaccactat cagaatttcc
     1561 tgaatgcctg tcagggcatt gagccggcca attcctcttt cgatgtggcc gctccgctgg
     1621 cggaactgct gtgtctgggc tgcatcggtc agcggttcgg cggcactctg ctgtacgatg
     1681 ccgaggccat gaaaatcacc aaccaccccg aagccgacca aatgctcaaa ggccctgccg
     1741 tccgcgacgg ctgggatgcc tacgaccgcc ggcagctggt caagtccaag gcctcgcaaa
     1801 tccaatcgcc cgacagcgtc ccgtgggaag atttgctggc cgatccggcc ttgagcagct
     1861 gggaaaatcc ttacgactgg ggcaaggccg aattcaagga tggcgttgtc agtctgacat
     1921 ccgaaaacgg caaatggttc ctgctgacca aaaaggaata cgccaatttc gtctttgaag
     1981 gcgaagtcaa aatgccgccc aatcagggca actcgggctt tatgttccgc tgccagaagg
     2041 cgaaaaaccg cgtatggggc tatcaggccg aagtcgatac cgccgaccgc aaatggtccg
     2101 gcggcctcta cgacgaaggc cggcggatgt ggtttgccag ccccaaccgc gatcaggccg
     2161 acaccgaaga ggaaaagaac cgctcgattg ccgaattccg cgcccgggcc ggcgactgct
     2221 acaagcaggg cgaatggaac aagtaccgga ttgtctgcat cggccctcat atccagattt
     2281 atgtcaatga catcctgacg acggatatct ttgatgaaat ggatctggcc ggaccggtcg
     2341 gcattcagca ccacggcgaa aagggattga cctatcagtt ccgcaatctg cgcatcaagg
     2401 acttgggagc cggcgggccg atttattacc cccaccgcga aggggcaaag gccgccgccg
     2461 tgccgtccaa aatggacggc gccatctacg aagccgaaga agccaatgtt ctttccgatg
     2521 ccaaggccgc cgacaaccac aaaggatacc aaggcaccgg ttttgccgat atgggcggca
     2581 aaggcagctt tatcgaatgg aacaatgtgc tggccgacac cgccggcagc tacacgctga
     2641 catttcgcta tgcggcggca ggcagccgcc cctgcgattt aaccgtcaac ggcgccaatg
     2701 ccggccggct ggcgtttgcc gacaccggaa gctggaccag ctggaaaacc gaaaccatcg
     2761 ccgtcacctt caaaaacggc ggcaacagcg tccgccttac tgccgtcggc gacggcccga
     2821 atatcgatgc cctcgctgtg acaaaatagg ccggacctga cgaaagcggc tgatacaaca
     2881 ccttcaagcg ggctgtcaac tggaagcccg cttatttttt gcccaatagg gcgcggcaga
     2941 taagcccaat tatgcggcgg gccgaagagg ctttcgggcc ggccggcaga acctgccagt
     3001 cgccgccgcc cagtttaatg cacagtgccg atgtgtctgc accgattgcc gacggcgtat
     3061 tggccacaat catatccaga tttttcgacc gcagcttctt ttcggcattc tggcggagct
     3121 tgcggtcttc caatgcaaag ccgaccagaa actgcccgct ctgtttattc cgtcctgccc
     3181 accggagaat atcgaccgtc ggcttgagtt tcagcgacaa gacggcctgc tcttttttga
     3241 ttttggtccg gctgaaacgg gcgggcttat agtccgacac cgcagcggcc ataatcaggc
     3301 agtcgcaggc ggcaaattcc gcctgcacag ccgccagcat ctcatcgctg gaaacaaccc
     3361 gtctgacccg aacgcctttg ggcaccgcca gagcagtcgg cgccgtaatc agcgttaccg
     3421 catggccggc ccggacggcc gcccgagcca gcgcatagcc catgcggccg gaactggcat
     3481 tggaaataaa ccgcaccgga tcgatgtatt cccgcgttcc tccggctgtg attaaaaacc
     3541 gcagcgatgg cgctgttttc ttcatctcaa tgggtcgtaa actcgccggc ttcccgaagc
     3601 aggccgacaa gatttgccat tttcttgctg tataggcctg ccgaatcctc cgccggcgta
     3661 tcatctgcat cggttatctt gttaaacacc gccgttgctt tcagcggcag cacaatctta
     3721 cattccgcat caatgtaaac aagggagtcc cagtccccca gtactgtata gaaacgggtc
     3781 gccggcgaga ataaatcaaa gcccaaagaa taatcccgcg gcggattgtc aacgcccaaa
     3841 agggtcatca gcgttgccgg tatatccaga tgactggtca tcttggcgta ttcagccgcc
     3901 ggccggccgg gaacccagag caccagcggt gtccgcacct gttcctccgt aaatcccgaa
     3961 ttgtgacccc agtgtccatg ctccataaac tcctctccgt ggtcgccggt gagaatcaca
     4021 actgttgaat ccagaagctg atgctgctgc agatagtcaa taatgcgggc aaactgcgta
     4081 tccagatgcc ggcaggaatt cacataccgg ttgaaaatcc gtccaatgtc ccgctgcagg
     4141 tcggctgtcg cataattgaa gtctttcagg taatccttgc ggatttcgca ttcgggcgga
     4201 aaataataac gggcatgcgg cgattcaaaa aacagaaagg tcataaacgg cttgtcttta
     4261 tcccgccggt caataaagtc cagcagacgg cctgtattga cgcggtctct ctcccagccc
     4321 tccccctgcg aatagtcatg cagatgctcc ggcggaatcc ggctgaacag cgttttgtca
     4381 aactccggat aggaaaaccg ggcactggtg taaaggtcaa tttgatagcc gttatcctgc
     4441 agcagatcca tcagaaccgg cgagcgctgc tcattcagaa agcggaacca gtaattgccg
     4501 tacaggccgt aaaacatact gaacatcgcc tgacaggtgc cgttgccgcc gctgtaatga
     4561 ttggtaaaat actgtgcccg ccgggcaaac tcccatgtgc ggggcatcac ctccggattg
     4621 agcatatcgg cacgccacga ttcggcaacc agccagacga tattccagcg cgggctgtca
     4681 ggacgccggc ggatgggctc aagcggatac tgaagcgcca gccccttaga cgaatggact
     4741 gtcagcagct ccctgcggcc cggctgaaag ccgaggtctt tcagaatgcc ctgaaaggtc
     4801 atcggcatat aaaacggaaa agcccccgag gccgccaaaa ttcccgcatt gcccctgaag
     4861 ctggaaatcc cgaaaaggag ggcctgcaat ccgaacatcc ccgccgccat aaacggcacg
     4921 gacactcgga gccgatgcgg ccaccggctc gctctgctta tgttccagtc ctcccaccgg
     4981 atacagagaa acagcaaaaa tatctccagt gccaaagcag ccagcaaaaa cagaccggct
     5041 gtccagtaca ctccctgttc aaaacccatc gattcaatgc cgcccttggt agtcagaaga
     5101 ttccacacaa aaccattgat gtgaaaatcg tacatccggt aaataatgcc gtcgatgaga
     5161 ataaccaaat gcgtcagcgt catcaccagt acggcggccg catacatcag caattgtgca
     5221 taccccttcc ggcagagctg gccaatccct atcaccccgc gcaccgcact cagcagcagc
     5281 ccggccggaa tcagatacac caaagcatag gccagcatga ccgagacaat aaagaccgcc
     5341 gcaccaaaat cggctttggg agcggcaatg acgaaccccg tcagattggc cagcatcaga
     5401 acatagttag cggcaaagaa gcgattgatg ctccggactg cgctcatttc gattttctgc
     5461 ttcatttctg gctttctccg ccaagcgata attgtcgctg cagccatttc ttgttgggct
     5521 gctgtatctc atgtcgtcag tcggatagga atgttattta aaaaattttc tgtatcgcat
     5581 caacaatctg gggcacttcg gccatacggc cgatgccttc ggtgccgcag gccaagcggc
     5641 ctgtttccgg tccaaccaga tggattttaa gtagatctgt caaaatagac aaattacgct
     5701 ggacaatggg attggcccac atccggctgt tcatggccgg agccagcagc acttttttgt
     5761 cccagcaggc acacagggtc gttgtcagaa ggtcatcaca gatgccgttg gcggctttgg
     5821 caatcaggtt ggccgtcgcc ggagcaacca caaccatttc cgcccagtct gctatctgga
     5881 tgtggccaat cttaaaatcg tcctgcccat gccacaagct cgtataaacc ggctggccgg
     5941 tcacggcttc cagcgtagcc ggcgctatca gccggcaggc attttccgtc atgattgtcc
     6001 gcacaacagc cccccctgcg gtcagcttgc tggccaagtc tgccgcttta taggccgcaa
     6061 tgcccccgct gatgcccagc aggatgcggc gcccttccag ttgtttggac tgctccggca
     6121 tcaacacacc taaaacggcg taatcttggg ttttttctcg tcgccggtta tatccagcgc
     6181 aatcttgccc tgcttgattt cctgcaccac gatttccatc tgggtcaggc cttcggtgtt
     6241 ctcaatcagg ggccggccgc cttccatcag ttcaagcatc cgcttctgaa tcagcgccga
     6301 cagcttaaac cgccctccga ttttgttgat gatttctgtg tctttgagtt cttctatcat
     6361 atgtttccct tacaaatacc gtctataata tccattactt cctgtatggc cgcatccaga
     6421 tcggcattga cgacaaaata gtcataatgc ttccgtgccg cggcaatctc tttctcggct
     6481 gtttcaagcc ggcgttttct gctggcttca tcttcgcccc gcccgcggca gcgcatccgc
     6541 tgctgcaggt cttccggccg gggcggcaga ataaaaatca gcactgcttc ggggcatttc
     6601 tgcctgacct gcaggccgcc ctgcacatca atttcaagaa tcaccgtccg gccttcggcc
     6661 agcgcctttt ccaccggcgg ccgcggcgta ccgtaatagt tgccgaatac atcggcgtac
     6721 tcgagaaact cgccggccct gatgcgccgc tcaaactctt gccggctgac aaaccaatag
     6781 tcccggccgt tttcttccga agcaccgggc tttcgcgtcg ttgccgaaac gctcaaaaac
     6841 gcatcccgtt tttcgacaat ccgccggcaa atcgtgcttt tgcccacgcc cgaaggcccg
     6901 ctgataacaa ccagtttgcc cgctgcctgt gcattcgtca ttgtccgctt tcccgtccgg
     6961 tcggccgcag ccggctactc gacattctgc acctgctctt tgagccggtc gatggcacat
     7021 ttaatctcga tgaccgactg actgatctgg gtatcggagc tcttgctgga aatggtattg
     7081 gcttcccgca gcatttcctg cgccataaaa tccagccgcc gcccgacagg gccgccggtc
     7141 tgacagcatt cccgaaactg ccccagatgg gccgacagcc gggagcattc ttcggcaata
     7201 tcgctgcgct cggcaaaaat cgccacttcc cgcgccagca gattttcatc aattttcaat
     7261 tgcgcctcgg ccagaagctg gctgatgcgg cttttgagcc ggctgtgata ttcctgcacc
     7321 actatatcca cccgctggcg aatcttgtcc agatgaaagg cgatgcggtc cagatggtcc
     7381 agcaaatccg ccgccagcgc ccgcccttct tcctgacggg acttctgcag cattgccagc
     7441 gcctcctgcg tcagcgccac aatcacctgc cgcatcttct cgatagacgc ttcatccgga
     7501 attgccggct gcacgattcc cggcagcgac agcagcgaag ccaaatcgat gcggctgtgg
     7561 gcatcgtcct catccagaat atccttgagc cgtcggatat aggtcttcag ggtgttttcg
     7621 tcgatatcga acagggctcg gccggagata ttcttcatcc gcagccagta gctgacggtc
     7681 ccgcggacga tggtgtcccg atggagccgt tcaatgtcgc tttcaagaaa agcggcaata
     7741 tccggcagcc ggaactgcgc cttgaaaaag cggttattga cagaccgaat ctcgacagta
     7801 taaaccacac cgtccacttc ggcacaggcc tgaccgaatc ctgtcatact gtatatcatt
     7861 gcaatgctcc gccgttaatt ggccggctcg ggtgtctcct gcaccggctg ggcaggggcg
     7921 ccggctgcgc tgtcggccgg ttggtcggcc ggcgtctggg cttccggcac cgctggggct
     7981 gcggcttcgg aagcggcttc ttcagacggc gctgctgccg gcatcagcgg ggcgctgagg
     8041 ttcagcgaac cggacggacg cataaacttg cccatcagca ccgccagcac cagaaacaaa
     8101 gcaaccagac aaatcgtaac ccatgtcaga aaatcacccg ttttcgtgcc cagcagactg
     8161 ccggccccgc cgccgccgaa ggcagcgcca atcccgccgc cgcggccttt ctgaatcagc
     8221 acaatcagaa tcagcgccaa acagacaagg ataaacaagc cgacaatcag gtttcccatc
     8281 aatgacaaag caagcacaca gctcatagat cttcctcata cagtactata tcgttttatg
     8341 caacggtttt gaccattgcc gcaaaatcct caactttcaa actcgctccg ccgaccagca
     8401 gcccatcgac atccggctgg ctcatcagct cggcggtatt ggacggcttg gccgacccgc
     8461 cgtactgaat ccgcatcgat tgggcaatgt cccccccgta cagctgggcc agcagattgc
     8521 gaatcatcag gtgaacttcc tgtgcctgcg ccggtgtcgc cgtccggccg gtcccaatcg
     8581 cccataccgg ctcataagca atcgtcaccg cctgcacgcg ctcgctgcag agtccatcga
     8641 gtcccttttt gacctgctcg gctaccaccg ctgtggtttt gccggcttcc cgctcctgca
     8701 gcagttcgcc cacgcagaaa atcggcagca gaccccccgc aatcgccgcc gctatcttct
     8761 tattgataag gccgtcggtc tcgccgagaa tatgccggcg ctcggaatgg ccgatgatga
     8821 cataggtgca gctgcagtcc ttgagcatct ctacgctgat ttcgccggtg aaggccccgt
     8881 tgccttcgaa atacacatcc tgtgctccca gtgcgatatt ggatgcgctc agcgccgcag
     8941 caaccgactg cagataaaca aacggcggac agaccgccac atccaccgat gcgatgccgg
     9001 ccaattggct ggacagaccc cgggccagcg ctgccgcgct ggtgctggtg gtattcatct
     9061 tccagttgcc ggcaataaaa ggttttctca ttgtttggtc tcctgtgtgt cagatggctc
     9121 tctaaagatt tataaaaccg tcgtactgcg aggaaaactg ccaaggaccg acagctgaag
     9181 acagtgcgat ttggcctctt ccagcgcttt cttaacattt ggatcctctt tgtggcccag
     9241 aaaatccaca aagaaataat attcccactc gcgcttcttg ttcggacgcg agccgatatt
     9301 ggtcatattg atgtcgtagt ccttgaacac attcagcaca tcaaccagcg cgccggtccg
     9361 gtgggcggtg ctgaacagaa taatcgtctt gtcgtcgccg ctgcgctggg catcctgctt
     9421 gctgatgatt aaaaaccgcg taatgttgtt ggagatatcc tcgatgtcct gacaaagaat
     9481 cttcaatccg tagatttcag ccgcaatcgc cgacgcaatc gccgcggaac cggcctcttc
     9541 ggcggccagc tgggcggcct tggacgaaga agccaccgga atcgtctggg catccttgaa
     9601 tgtagccgcc agccagttgc ggcactgggc aaacacttcc ggctgcgaat aaatccgcgc
     9661 gatctccctc atcgggcagt tggccagcag gttgtgatga atcgccatat tgacttcggc
     9721 acagaccatc acatccgttt ctataaatgc atccagcgac tcgcggacgc ccccgccggc
     9781 gctgttttct atcggcacga ctccgaagtc gcaatgtccc ttgctgactt cctcaaaaat
     9841 cccccgtata tcggccagcg cctcgtaatc gacgctttgg ccgaacttca ggatcgccgc
     9901 attgtgcgaa aagctgccgc gcggccccag atacccaatc cgcagcggcc gttccagaaa
     9961 gaaagacccg ctcatcagct cccgccagat ggcctgcaaa gtcttgtccg gcagcggacc
    10021 tttgttgatt tcacagatgc ggtcgagcac ttctttttcc cgatgcggca tatacacggg
    10081 gctttcgctg gcctgcttga gcttgccgat ctccacgaca atgcgcgcac gttcattgat
    10141 cagctcgaca agcttggtat ccagcgcatc gattttttta cgcagttctt ccagcgacat
    10201 tggctgcttt ccaagccggt tcctatccag accggcgttt gaatagagta tctccgcctt
    10261 aaacggaaac gattttcata ccaaaataac ttcccttcgt caatcaaaaa accgctctat
    10321 tcctgcaata aaaccttgaa tttgattatt ttatgatgaa aataaaccct atgacgcaac
    10381 agcagaatta tttctattcc atattcaaga cccattgggg ctggtttggc ctgttgggtt
    10441 ccgataaagg acttatacga acctgtttgc cgactgcttt caaagaagca acacagcagc
    10501 gtattttatc gggcattccg gcggccgtac ctgccccaaa aaccttccgg cagctggaag
    10561 aacagattat ctcctactac aaaggacatc cggctgattt ttccagcgca gcggtctgtc
    10621 tggaaggcct gacagagttt cagcggcata tcttaaccat tctgcgaact ataaaatatg
    10681 gccaaacaat tacttacagc gaattggcac ggctggccga ctgcccccgc ggcgctcggg
    10741 ccatcggggc tgtcatggcc gtcaatcccc tgccgctgat tatcccctgc catcgggtca
    10801 tcaaacagga cggctctctg ggctatttca gtgccgccgg cggcgttgat acaaaaaaac
    10861 ggatgctcga acttgaaaag gctgccattt aagtccgctg atgcagcgct gggttgattg
    10921 ctgcataact ccatatctga taatgtctta caagattcac actggtacaa gttgaccgcc
    10981 ggcagataga ttggtcaaag gccggctcga ttgcctttga tgagccggac gaagccatta
    11041 tgacccgcgc ggctaaaatc gcttttatca gccagtttta cattttcggt gccggttgcc
    11101 tgtgtccgct gctggttatc cgaagcggga ctgttccggt gatgtatctt gttgggctgt
    11161 tccttgtcgc tgcggtcttg tttggcatca tctggtccgt tgcggtcttt tgtctgtacg
    11221 gcaggacaac cgatgcctga ccagcggctt agcaatcaaa tccgccggct gcggtttgag
    11281 gcgggggaaa tgacccagca ggcgctggcc gacagggtcg gcgtaacccg tcagaccatc
    11341 attgccatcg agcagggccg ctatgcgccg tcgctgccgc tggccatcaa aatcgcccgc
    11401 accttcggca aaaccgttga cgaggttttt cagctggcct aggccggctg ccgggacaat
    11461 cggtccgcag gcggcgggtt cttccggccg gctttaccgc tggaccagat accacatcgt
    11521 gccgtattcg gctttaagcg ggacgatgcc gttttcgatt ggaatctggt ccttgaccgt
    11581 gccgtccgga tggagcggtt tcagtgtggc gctctgaccg gccggcaggg gcaggacaag
    11641 cgtccctgca accggttcaa tccgcaccgg cgccgtcccc cagtttcgcc cgacggtccg
    11701 gcggtcgggg gaaaactgca tccccgtatt ttcacaccgc ccgcaggccg taatgaggat
    11761 tgtgccgctg gcagacagcg gcaggccgtc caaagccgtt atgacaaccg cggccgaatc
    11821 gggctggtct atttgaagcc ggtgcctgtc cttatccagc cggctccggt ggccgcagaa
    11881 gacttcgcag cggctgttgt gcatccaata cagcccgctg ccgtcctgtg cagtccattc
    11941 cagaaccgcc gcatcgtcag agccgccgga tgccgtttgc acgggtccgt ccggctggaa
    12001 cgtatgataa aacgcatcca gcttctccgc cggcagacga cgcaggggcc cggacagatc
    12061 tctttcaatc cgcagatgca gctctgccag agcacccagc agcgacccat ccgaagacac
    12121 cagcggcacg gctgttttcc ccgcaaacgg ctccagcgcc ctttggcggt acatcacagc
    12181 gccggcgggc ataaagcccc acttggccgg attggtatcg acatcaaaat agctgttcag
    12241 gtgctgccgg ctgaattggt tgttgctgtg gctgtatgta taaatccaca cgccgtccca
    12301 gtcctgtgcg gcagcaaacg aagcaatcat cggcacgcac tcggcctgcg agtccagcgg
    12361 ggccgggtgg ttgtactccg taacggtaaa cggcttgccg gccagacgct cggcagccag
    12421 ctcgtacagt gttcccggcg gattgccgct catcgccttc tgctctatca gccagtcgcc
    12481 ggaatcccac ggctggcggg gaaactgcgg atgctgccag taggcgtgac tgtcgataaa
    12541 atccattccg ctttgggcaa acagccccag cggcccgaag acaatcgttc cggttaccat
    12601 cgccctgcag ttcaggtcgt ttttcagaaa cgcccgcatc tggtcaaaat aggccttctc
    12661 cgtctcggcc agaaaacaca tctggtctat ctgccgcgcc ggaaccggac tttctgcaaa
    12721 cagcgcaacg gtcgcctcct ccagcgattc gcccggtgcg acgccgatct gcccgccggt
    12781 gcgcagctcc accgtgccca gctggaccgt accgccgtcc atgccgaagg caagattcag
    12841 ccggccgttg gcatcgccgg cagcggcggt aaagcccagc gtaaaggtct gccagtccgg
    12901 cgtcagttgg atatcgcggt acagccccag attgctccac ggctcgtggg cctgctggac
    12961 gctgacattc agcggccgcg gtctcggggc gcgggccttg aggatgacgg tgtaaaattg
    13021 cccctgttca aatgtcagcc ccggatggtt gtactgcaaa tgccatgcga tgccgtcggc
    13081 gttggcgatg tcaatcgtca ggccggccgc ctccgcagcc ggcgacgaca cggcggcttt
    13141 ggcctgctcg tgctgctcca agcgccagcc ctccaccggc agcgaagccc gcagaagatt
    13201 gaccggcccc agcggctggg cttgcttgtc ccaaacctgc cgcagccggt cggttgtgcc
    13261 gtatttcctt ttgagccatt ggctgtattg gctgcggaga atatcggcat aaaacggcgg
    13321 cagggcgcgc aggctctttt ccgaatccca cataaacaga ctgttctcgt tggtaatctc
    13381 cacgatggcc acagcataat cctgcgcata ggcaacatgg ctgcgatagg gattccgatg
    13441 cgtcagcagc aggcgggcat actccttttg ggcctctatc agctgcggcg taaagaggct
    13501 tgccattttg ttgtattcgg tggccggatc gggcaggccc agtgcctcgc tgtgttttcg
    13561 gcccacctgc agattcagat tgacatagat gccctgtccg gcaagctggt cgatgaaata
    13621 atccagccgg tccagcgcct ccggatgcag agcccggccg gtttggtccc agatgccccg
    13681 cggccacgaa gcggtatcca tatgatggca gcggacggca ttgaccccga aggccgccag
    13741 ccgccgggca atctcccggg catccgaatg cgtcggaaaa ttggcttcaa aggtaaaatt
    13801 caccccccac agccgcaccc gtttgccgct ttcggtcaga aaatgcccgt cggcgtgcag
    13861 gcggtcctgc tccgtcagcg gcgcttgctg catgcggata gccgaatccg gatgcacgcc
    13921 ggcaggaatc acaaagggca ccatatccgc tgccgccgcc ggcagacacc ccatcagcca
    13981 caccacaagc aatcgcttca tcgccgtctc cttgattttc gttttatctg aataacgccg
    14041 gcggaggact gtcaatttca atttttcaat agaatttcgc cccgctctgt gttatggtac
    14101 agcaaaatgc aaacagcagc attcgaggat ggacgcctat ggcaaaagac aaaacccggc
    14161 agatctgcat cgtaaccgtt accggcaagg ataaagtcgg catcatcgcc cgcatcagct
    14221 cggtcatggc caaagccaat gtcaatatcg tggacgtcag ccagcggatt atggaagact
    14281 acttcgtcat gacaatggcc tgcgacatca ccgacgccac cgcagacatg gacaaactcc
    14341 acaggcagct gtcccggctg ggcaaggaac tgcacctgac catcaccttc cagcacgaaa
    14401 atatcttcaa gatgatgcac cgggtataag tgccttactg acagccgttc agcggccgct
    14461 tgatgcccca cggctgcttg atgccgccgc ggccgttgct ggcgtggaaa tcgacaatga
    14521 tttggacggc cttttgcggc tcatcgcaga cggtaaacac atccatatcc tccggcgcga
    14581 tatacccgtg ccgcccgagc atcactgttt tgatccaatc gaccagtccg ccccaatagt
    14641 cgctgcccat gcaaatcaca gggaaatacg cctgcttgag cgtctgaatc agcactagcg
    14701 actcaaagaa ttcatccatc gtgccaaagc cgcccggcag cacaacaaag ccgtgggcat
    14761 atttgagaaa catcaccttg cggcagaaaa aatagcgaaa atgcagcgac aggttctggt
    14821 atttattggg tatctgctca tgcggcagct caatattgag cccgacactt ttgacccccg
    14881 cctcgcgggc gcctttgttg gccgcctcca taatcccccc gccgccgccg gtgatgacag
    14941 caaaaccggc cttgcccagc tgacgggcgg tttcagcggc catctcataa tagcggctgc
    15001 cggctgtttc gcgggcggaa ccgaatatcg atacagcagg gccgatattg gccagttcct
    15061 caaacccttc cacaaactcg gccattatcc gaaatactcg ccacgtttct tcggctgata
    15121 cataaaaatc agacatcgct tctccgttat acccgaataa cctctttggt ttttccctgc
    15181 ttatcttatc ggaaaatccc tccaaacgct aaacctttca atccccaatg caaaaaaaac
    15241 gacctatctt ttatcggcag gctgcatttg accccgaaaa gtcctctgct aacccgacag
    15301 atattgtcca gatggtccca attctgcatg cactatcttg ctatcttcaa aaatccgtaa
    15361 aaatcttaaa tcccttatat gcaagcagtt atcgaaattg tacccccttg c
//
LOCUS       DAOTBO010000069        15334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3237_length_15334_cov_4.434911, whole genome shotgun sequence.
ACCESSION   DAOTBO010000069 DAOTBO010000000
VERSION     DAOTBO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3237_length_15334_cov_4.434911"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLE88_08705"
     CDS             complement(<1..394)
                     /locus_tag="PLE88_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76580.1"
                     /translation="MSPLLKDQGLYEVRREHDACGIGAVVHISGKKDHSIVEYSKQIL
                     LNLHHRGAASADDVTGDGAGILTQIPDAFFRMETQRLGICLPEAGRYGIGMVFSPKDT
                     GLRSCCYGLLEQAAAHYGLRVLGWRCVPA"
     gene            complement(627..2723)
                     /locus_tag="PLE88_08710"
     CDS             complement(627..2723)
                     /locus_tag="PLE88_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOM76581.1"
                     /translation="MENKQAELIELFGANVFNDAVMRQRLPKNTYKEFTRIRQGGGEM
                     TASLADVIANAMKDWAIEKGATHYCHWFQPMTGLTAEKHDSFISPTPDGKTIVEFSGK
                     ELIKGEPDASSFPSGGLRATFEARGYTAWDCSSPVFIKGRTLYIPCAFCSYTGQVLDK
                     KTPLLRSMEALSKQAVRVLKALGKTDVQNVITTLGPEQEYFLIDRSYFERRLDLVLAG
                     RTLFGAPSPRGQEMEDHYFGTVHERVAAFMNDVNKELWKLGVSAKTQHNEVAPAQYEL
                     APVFSTVNVATDHNQLTMETLQKVALRHNFVCLLHEKPFAGVNGSGKHNNWSMVTDTG
                     INLLDPGSTPHDNMVFLVFLCAVIRAVDKYAKLLRASVANPGNDHRLGANEAPPAIVS
                     IFLGDQLTDIFKQLAGGGLKSSQKSSELKLGTSILPPLPKDTTDRNRTSPFAFTGNKF
                     EFRMVGSSQSTAGPNFVLNTIVADVLMEIAEELEKSKNPATDAQKLLKRIVTEHLRII
                     FNGDNYSQDWVEEAQKRGLPNIRSAVESIETLMDKEHVSVLTRHGVLTKNELHARMEI
                     LLENYSKTIAIEANTMLTIAKRQILPVCIEYCGRLASAATVIAEAGVTSRSARKNLEK
                     ASQMTDELHNAIEALEAVASKAKKNGNAVQTARYYRDEVVSAMQTVRKAADALELLVD
                     ADLWPLPTYAEMLFLK"
     gene            complement(3162..4301)
                     /locus_tag="PLE88_08715"
     CDS             complement(3162..4301)
                     /locus_tag="PLE88_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76582.1"
                     /translation="MTQYPAFYLISTAGFPNLGDEWITLGWLEAIFRKYPQAAVFLDV
                     HFPSGFDSLIRTRPYAGRVCCVDFFWQLTYPYSGQPWEDCLRNFQDAYACRRPAALRH
                     LSAILPRTSHLHFLGGGHVTGIWQHHLLLIVLAGLLKDDCRLPVYWTGGSLQPIEDVH
                     LQGLLPFLSRFDFISFRDSESARQVNSCLPGTVCPSCDDIVLGLSEESVQPPAASERP
                     VVLVNLQSDFCPDDSFGAIGQTLIEKICSFRQHSVLFWEMNPQCDRKGYQFLASRIPQ
                     MQLISFEALWKSTALGGMPEAIVHPQSYALGSRFHFHYYLAWHGIAGEFISQTSYYDT
                     KHKSICEAGSGWRPLHLAAEKTVQPRLDKRHLSQLKRREFDRIYG"
     gene            4500..5753
                     /locus_tag="PLE88_08720"
     CDS             4500..5753
                     /locus_tag="PLE88_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOM76583.1"
                     /translation="MNFLLLAIDAVLLYGMFLLAYLIRYGQNIPPASFAPFRDSRLFL
                     VGIILAALVQAGVFRRRFRSYWQLFQKLFYGLLAGTVLDFVLMYLLRDRWMRFPSSIL
                     LLQFGLSLAVLFFVNGLLLRLKNRIQKRVVILGSSEFHDPFLKKSALVRLKHITAIEE
                     LMHIRDVDEVMICERLQDEKNLNLLIFLLLKLNVTVTFGPAVYAELIAGRIQQDNIVH
                     LISTFLGRKSDGEEFMIRLLDIVGSLLLLVIFSPVFLAAACWVKLTSPGPVIYRQVRV
                     GKDRQLFTIYKFRTMRNDSDKLYGYKPAVSGDQRFTPVGRFLRRTRFDELPQLFNVLK
                     GQMSLVGPRPENVWRVNTHKALRGLRLAVKPGLTGLAQIRSYYDVSPGHKVKYDYLYI
                     QRRSFMLNLYILLKTIPVVLTQKGV"
     gene            complement(5756..7243)
                     /locus_tag="PLE88_08725"
     CDS             complement(5756..7243)
                     /locus_tag="PLE88_08725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOM76584.1"
                     /translation="MKTFSDNRQKMGDMILLAAAAAVLGAFLIARTTVISEDGVVYIE
                     RARQLPDNYQNVASRHEPIGLPVLIFLCHGFFLQLTGGDSPALWCLAGQVCVLGCRIL
                     TIAVLYLWASRLFGRQHAVQAMLALIVLPYPAQAGADVLREWPFLLFLSTSLLLFCQG
                     SQTLNGVLLGVSGLSCGLGTLIRPECAQVLLYGLFFFAYKTLRGVMEKKPLQQNGCYI
                     WLAAGFLMVFLPYALLTKQVVPHKLNQLLKSPSAAFGESILLTESASNAVETAGFNPA
                     DLSKGLAEAVNKIIINFLYYFAVPAGIGFYLFYKSPHKTDAHWLWALLIGFYLTVLCV
                     LYMRWGYISRRHLLPATALLSLHIPAGLNWIACRLVNGGQDQNHRQTWLRILLAAGIL
                     ICIPKLLKPLGHDKKAYLAAARWLAENTTPASRIYTFDRRIPFYADRPYRLYRNSARF
                     RADRKAEYLIVPSGVPAELEDIILLEKTFSVSGSSQPVVILKRRR"
     gene            7369..8586
                     /locus_tag="PLE88_08730"
     CDS             7369..8586
                     /locus_tag="PLE88_08730"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM76585.1"
                     /translation="MTLYPADYIILAAIVMQGLFTIQVVNNCRYALEKIKRRRELYRP
                     RCVLIVPCRGLDEHFDENIESFYRQDYEGYHLWFVVQDRSDPAYERLLRLKARHRQNT
                     QAQSVKILVAGHTDSCSQKLHNLLYACRQIPPETEALVFADSDACAGPHWLSHIVYPL
                     RKDKNGAAGGYRCFVPQTNNLATVALSAVNAKICQLLGNTRFNLAWGGSMAILVKTFK
                     ELGIDKVWEKALSDDLTLTRAIRKKGLKMVFVPACMIASYESATWASLWEFARRQFII
                     TRIYTPGMWLFGLFSALFTVGGLWGGLAAAVWAVQTDYPHSGLVIALPAVFLACQLLR
                     AILRQRLIAKLLPADKERLRIARWADILFFWLWSILLLAVILSSAIGRTLVWRNIRYR
                     IESPVCVHILGRS"
     gene            complement(8610..9629)
                     /gene="aroF"
                     /locus_tag="PLE88_08735"
     CDS             complement(8610..9629)
                     /gene="aroF"
                     /locus_tag="PLE88_08735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOM76586.1"
                     /translation="MIIVMKPGATKEQIQHVIALVNDYGLKEHPIYGTDRTVIACLGD
                     KRAVNKDAIENAPMVEKIVPILSPYKMASLEVKKERTQIPIGPKGFPIGGPAVGIIAG
                     PCSVEDRRQIMTTAQAVRQAGCIGLRGGAYKPRTSPYSFQGLGLEGLKILAEAREETG
                     LAIVTEVIGLEQLEPVAQYSDVLQIGARNMQHYPLLEAVGKVQKPVLLKRGMSASIDE
                     FLLAAEYIINAGNPNVILCERGIRTFEEYVRNTLPLAIIPALRERTHLPVIVDPSHGT
                     GHAYMVPPMCLAAIAAGADGLIVECHPDPEHAASDGAQSITPQTLSQLMPKLRRVAEA
                     VDRTL"
     gene            9741..10433
                     /locus_tag="PLE88_08740"
     CDS             9741..10433
                     /locus_tag="PLE88_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOM76587.1"
                     /translation="MIGQMNPATIKLLVLADIHGRHEKIASLRPLVKDADAVILAGDI
                     THFGGANETAEVLTALEPLGKPVFAVHGNCDQPVVQEVLSRRGISVHGCTKELMGLYF
                     AGAGGSLGWSGATPGEVGEAAFAASLQQAAAGLNAPSRLVLITHQPPWGTTLDVQGGM
                     RHTGSRTIRSFIEDHQPLLAVSGHIHEARGIDRLGDTVLVNPGPLRSGCYAAVEITNC
                     DVQVRLGLLDSA"
     gene            10452..11261
                     /locus_tag="PLE88_08745"
     CDS             10452..11261
                     /locus_tag="PLE88_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOM76588.1"
                     /translation="MNHFEQLHPVVQAFLATLFTWALTALGAASVFLTKTISRRLLDF
                     MLAFAGGVMIAASFWSLLAPGIEMAQLQGQTAWIPAAVGFLLGGAFLRLMDRFLPHLH
                     LGYPMSEAEGVHTRWRRTTLLVMAITLHNIPEGLAVGVAFGALRYGLSSASFTAACAL
                     AMGIGLQNLPEGMAVSVPIRQMGTSRLKSFWYGQLSGMVEPIAGLIGAAAVVLVSPIL
                     PYAMGFAAGAMIYVVVEEVIPESQRAGNTDLATVGTMVGFAVMMTLDVALG"
     gene            complement(11786..13381)
                     /locus_tag="PLE88_08750"
     CDS             complement(11786..13381)
                     /locus_tag="PLE88_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOM76589.1"
                     /translation="MRKCASLFSFVSAVIACAGLSVRADGQAVNWQGPSNSRIKQIRY
                     FHKEPAAAQALAAPAPSAAVSGVIDSPPVDGFVPWIVLTPTNKHLDAETTGIYEAFPS
                     SYAGSPPAGTNPRTDFMIAIFDTGASSHVIGYYNAVRAGLYNSTYLTQKNKAEVTGVT
                     GSVEARITWPYALFMAGLDVLEPNAPGQSEMILPSTAGLLGQYNVATLIGQNPGTKPD
                     LATAVGTPMSVFYDTHIEVDHPITITRSQTVYTGPRITFYPKGSPSPAYADYIPLELK
                     PAGSTTVQYITYGFNPEDLLEMFTDPWGFEMDYSPMTPSVVMGVSSQSLFFIHGVDLT
                     DSGRSAVDKNRFMLDTGAQITVIGSRIAARLGLQAAGRDFEVEIEGVTGDSIMAPGFY
                     LDRLTIPAVGQWLEFTNVPVVLLDIFSPEGGTLDGIIGMNLFTQYNLILRAGGFMLED
                     DPRLEFQRIAAAGQTADIAPLPLDGKVDMQDLAAFSSAWQGSGLTVWNTSADLAPLNA
                     PDGAVDLADLSVLAENWLAGLLY"
     gene            13712..14047
                     /locus_tag="PLE88_08755"
     CDS             13712..14047
                     /locus_tag="PLE88_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOM76590.1"
                     /translation="MGIQNWSENVILVNLCKEPDLGEELQTVIEMVGADTASDVVLDF
                     ADIDIITSSSIAKMLKLRKVLRDNGRTLLFSSVGPKTKSIFNVTGLDTVFEFVDDQFT
                     ALASLQMVN"
     gene            14207..14914
                     /gene="rnc"
                     /locus_tag="PLE88_08760"
     CDS             14207..14914
                     /gene="rnc"
                     /locus_tag="PLE88_08760"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOM76591.1"
                     /translation="MDAQTVRRIEEALGYQFKNPQLLAEAFLHASAADDRRRSNERLE
                     FLGDAVLSLVVCQTLFERFPDYLEGDLTKIKSRLVSRKVCAELAHQLGLPGHLRLGRG
                     MESSRALAGSIAAASLESAIAAIYLDGGMEAAKTFICRLYEPLFVEADAQQHQENYKS
                     LLQQYCQQEYGCMPIYDLLDEKGPDHNKCFEIAVVVRHLRFDSAWGITKKEAEQKAAY
                     NALCQLGLMGGPAAGSD"
     gene            complement(14911..15153)
                     /locus_tag="PLE88_08765"
     CDS             complement(14911..15153)
                     /locus_tag="PLE88_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76592.1"
                     /translation="MNEWIVAAFTAPEQIGTTPGSLLWMFPLLAAIAIVYKATKMRAL
                     LWRRFLVESAVLFATVSGFMILAIVVLNLLSWLITG"
     gene            complement(15146..>15334)
                     /locus_tag="PLE88_08770"
     CDS             complement(15146..>15334)
                     /locus_tag="PLE88_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76593.1"
                     /translation="VGILGSFTKNGRFPTYGRPCDIGQIYTGIAGLLNLLSILSAVYM
                     AYCGRGELIGQEEDGVNE"
BASE COUNT         3418 a         4115 c         4250 g         3551 t
ORIGIN      
        1 ctgccggcac acagcgccag cccaaaaccc gcaagccgta atgtgctgcg gcctgttcaa
       61 gcagaccata gcagcagctg cgaagccccg tgtctttggg gctgaatacc atgccgatgc
      121 cgtaccggcc cgcctcggga agacagatgc ccaaccgttg ggtctccata cggaaaaatg
      181 catcgggaat ctgggtcaga atccctgccc cgtcgccggt gacgtcatct gcgctggcag
      241 caccgcgatg atgcagattc aaaagaattt gtttgctgta ttctacaatc gaatggtctt
      301 ttttccccga gatatgaacg acagcgccga tcccgcaggc gtcatgttcc cggcggactt
      361 catataaacc ttggtctttc agtaatggcg acatctctaa tcctaatttg cagtctttat
      421 tttaaccgaa gcaactttaa gcaattttgg tatcactgac aaattattta taaaatatat
      481 ttatttttct attcatttta cttataaata ttctgacacc ctaacctttg atcttaatat
      541 ttcacaaaaa aaaccgccgg cattgtataa ccggcagggt atgtttgtcc taatgttaag
      601 tacgacagct gcggctgctg cggaatttac ttcaaaaaca gcatttctgc atatgtcggc
      661 agcggccaca agtctgcatc taccagcaat tcgagggcat cagccgcctt tcggacggtc
      721 tgcatggcac tgactacttc atcgcgataa taccgtgctg tctgaacggc attgccgttc
      781 tttttggcct tcgaagcgac ggcttccagc gcctcgatgg cgttatgaag ctcatctgtc
      841 atttggcttg ccttttcgag gtttttgcgg gcggatctgc tggtgacgcc ggcctcggct
      901 atgacagtag ccgcagaggc cagacggccg cagtattcaa tgcataccgg caaaatctgc
      961 cgtttggcta tcgtcagcat cgtattggcc tcaatggcga tcgttttgct gtaattttcc
     1021 agcagaatct ccatccgggc atgcagttcg tttttcgtca aaaccccatg acgggtcagg
     1081 acggacacat gctccttatc catcagcgtt tcgatggatt ctaccgcaga acgaatattc
     1141 ggcagacctc tcttttgggc ttcctcaacc cagtcctgcg aatagttatc gccgttgaag
     1201 ataatacgca aatgttccgt aacgattcgc ttcagcagct tctgggcatc ggtggccgga
     1261 ttcttggatt tttccagttc ttcggcaatt tccatcagaa catcggccac aatggtattg
     1321 agaacaaaat tcggtccggc cgtggactga ctggacccga ccatccggaa ctcaaacttg
     1381 ttgccggtaa aggcaaacgg cgaggtccgg ttgcggtctg tggtgtcctt cggcaggggg
     1441 ggcagaattg aggtccccag cttcagttcg ctgctttttt gcgagctttt aagcccgccg
     1501 ccggccagct gcttaaagat atcggtcagc tggtccccga ggaaaatgga gacaatcgcc
     1561 ggcggtgcct cattggctcc gagacgatgg tcattgcccg gattggccac cgaggcacgc
     1621 agcagtttgg catacttgtc cacagcacga atcacagcgc acagaaagac cagaaaaacc
     1681 atattgtcgt gcggcgtgct gccgggatcc agcagattga tgccggtatc ggtcaccata
     1741 ctccaattgt tatgcttgcc ggagccgtta acgccggcaa agggcttttc gtgaagcaga
     1801 cagacaaaat tgtgtcgcag ggcaaccttc tgcagcgtct ccatcgtaag ctggttgtgg
     1861 tcggttgcta catttacggt tgagaaaacc ggtgccagct cgtattgggc cggcgctacc
     1921 tcgttgtgtt gcgtcttggc ggatacgccc agtttccaca gctccttgtt tacatcgttc
     1981 ataaatgcgg ctacccgctc atgcaccgtg ccgaaatagt ggtcttccat ctcctgcccg
     2041 cggggcgaag gggcgccgaa cagcgttcgc cctgctaaaa caaggtccaa ccgccgctca
     2101 aagtagctcc gatcgataag aaaatattcc tgctcgggtc ccagtgtggt aataacattc
     2161 tgaacatccg ttttgcccag agctttgagc acccgaacgg cctgtttgct caacgcctcc
     2221 atcgaacgga gtagaggagt ctttttgtca agaacctgtc ccgtatagga gcagaaggcg
     2281 caggggatgt agagggttct gcctttgatg aaaacaggcg agctgcagtc ccacgctgta
     2341 tagccgcgcg cctcaaaggt cgcccgcaga ccgccggatg ggaaagacga tgcatccggt
     2401 tcgcccttaa tcagctcttt gccgctgaat tccacgatgg tttttccgtc gggtgtcggc
     2461 gagatgaacg aatcgtgttt ttctgctgtc aggccggtca tcggctgaaa ccagtgacag
     2521 tagtgggtag cacctttttc aatggcccag tccttcattg cattggcaat gacatctgca
     2581 agcgaggcag tcatttcccc gccgccctga cgaatgcggg tgaattcctt gtaggtattc
     2641 ttcggcagac gctgccgcat aaccgcatcg ttaaagacat tggcaccgaa cagttcaatc
     2701 aattctgctt gtttgttttc catttgcttt cctttcgcgc atttgggcgg aaccttcatc
     2761 aatatgcctg ccggcgtatc gcgaccaagc tacatcctat aaacacacct attagaaagt
     2821 caacaacaac tgccggaaac tcatgtttgg cctgatacgc ctttcagacc tgtacaggct
     2881 aaaactcgat tatatctatg tgagcaaata gaatgccaat ttttagaaaa aagcagatga
     2941 tttgtatgtg tttacagatg aacaagttac ggcgttttcg taaagtgcga taatttttca
     3001 aggattacaa ttttgtaatc atttggtatt aaagcataca aaaatgttga tttttcggct
     3061 ttcactgctc tttatgggtc ttctgaaaga cagctgatct tcttttgcct gccaaaaacc
     3121 ctaagaaggc attctttccc ggaaacgccc gcctgcggat ttcagccgta gattctgtca
     3181 aattcacggc gtttcagttg agaaagatgt cgtttatcca gccggggctg aacggttttc
     3241 tcagccgcca gatgcagagg ccgccagccg gagccggcct cacagatgga tttatgtttg
     3301 gtgtcataat acgaggtttg ggagataaat tctcctgcaa tcccatgcca ggccagataa
     3361 taatgaaaat gaaaacgaga tcccagtgca tacgattgcg gatgcacaat ggcttccggc
     3421 ataccgccca gagccgtact tttccagagg gcttcaaaag agatcaattg catctgcgga
     3481 atgcggcttg ccagaaactg gtaccccttc cgatcgcatt ggggattcat ctcccaaaac
     3541 agcacagagt gctgccggaa ggaacagatt ttttcgataa gagtctgacc gattgcgccg
     3601 aaagaatcgt ctggacagaa gtccgattga agattaacca gaacaacagg acgttcagat
     3661 gccgccggcg gctgcacgct ctcttctgac agtcccaaaa caatatcgtc gcagcttggg
     3721 cagactgtgc cgggcagaca ggaattcacc tggcgggcac tttcgctgtc gcggaaactg
     3781 ataaagtcga accggctcaa aaacggcaga aggccctgca ggtggacatc ttcaatcggc
     3841 tggaggcttc cccctgtcca gtaaaccggc aggcggcagt cgtcttttag caggccggcc
     3901 agcacaatca gcagcagatg atgctgccag attcccgtaa catggccgcc tcctaaaaaa
     3961 tggagatggc ttgtgcgggg cagaatggcc gacagatgcc gcagggcggc tggccgtcgg
     4021 caggcatagg catcctgaaa attccgcagg cagtcctccc acggctgacc cgaatagggg
     4081 tatgtcagct gccagaagaa gtcaacacag cagaccctgc cggcataggg gcgggtgcgt
     4141 atcagcgaat cgaaacccga cggaaaatga acatccagaa aaacagcggc ttgcgggtat
     4201 tttctgaaaa tcgcctccag ccagccgagg gtaatccatt cgtctcccag attgggaaaa
     4261 ccggcggtgg atatcagata aaatgcggga tattgtgtca tttaattatc cgctgggcag
     4321 acgctggcgg attcaccggt ctgcatcggc ttccattgtt gcacggttga aacactctta
     4381 aaaacactat tgtaaatgcg cctgccggct tgtcaaatgc atttctgaaa tctccttgat
     4441 ttgaagtctg cttgtcatac aatgacaggt gttgcattgt aaacggatgc tcaaacggaa
     4501 tgaattttct gctgctggcc atagatgcgg ttctgttgta tggaatgttt ctgctggcgt
     4561 atctgatacg atacggacag aacatcccgc cggccagctt tgcgccgttt agagacagcc
     4621 ggctgtttct ggtgggaatt attcttgcgg ctctggttca agccggcgtt ttccggcggc
     4681 gattccggtc ctattggcag ctctttcaaa agctgttcta tgggctgctg gccggaacgg
     4741 tattggattt tgtcttgatg tatctgctgc gcgaccgatg gatgcgtttc ccctccagca
     4801 tcctgctgtt gcagtttggt ctttcgctgg cggtgctgtt ttttgtcaac ggcctgctgc
     4861 tgcggctgaa aaacaggatt caaaagcgcg tcgtcattct cggttcttct gaattccatg
     4921 atcccttcct caaaaagagc gctctggtcc gcttgaagca catcactgcc attgaagaac
     4981 tgatgcatat tcgggatgtg gatgaggtga tgatttgcga gcggctgcag gatgaaaaaa
     5041 atctgaatct gctgattttt ctactgctga agctgaatgt aaccgtcaca tttggaccgg
     5101 cggtttatgc tgaactcatt gccggcagga ttcagcagga caatatcgtc catctgattt
     5161 ccacttttct gggccgcaaa tcagacggcg aggaatttat gatacggctt ctggatattg
     5221 tcggcagtct gttgctgctg gtgattttca gtccggtttt tctggctgcg gcctgctggg
     5281 tgaagctgac cagtccgggg ccggtgattt accggcaggt gcgtgtcgga aaggatagac
     5341 agctgtttac aatctacaag ttccggacga tgcgtaacga ttccgacaaa ttgtacggct
     5401 ataaacctgc cgtcagcggc gaccagcgct tcacaccggt cggccggttt ctccgccgaa
     5461 cccgattcga tgaactgccc cagcttttca atgtgctcaa aggccagatg agtctggtag
     5521 gcccccgccc ggaaaatgta tggcgggtca atacccataa ggccttgcgg gggctgcggc
     5581 tggctgtcaa acccggcctg accggccttg cccagattcg cagctattac gatgtcagcc
     5641 ccggccataa agtcaaatat gattatctct atatccagcg ccgttccttt atgttgaacc
     5701 tttacattct tttaaagact ataccggtcg tactgaccca gaagggtgtg taaatttacc
     5761 ggcgccgctt caagataaca accggctggc tgctgccgga gacagaaaag gttttctcaa
     5821 gcaggataat atcctccagt tcagccggca caccggaagg aacaatcaga tactcggctt
     5881 tcctgtccgc ccgaaaacgg gccgagttcc ggtacagccg gtacggccgg tcggcataaa
     5941 acgggatccg tctgtcgaat gtataaatcc ggctggccgg cgttgtattt tcggcaagcc
     6001 atcgagccgc agcaagatac gcctttttgt cgtgccccag cggtttgagc agtttgggga
     6061 tgcagatgag aatcccggcc gccagaagaa tccgcagcca tgtttgccga tggttctggt
     6121 cttgaccgcc gttaacaagc cggcaggcaa tccagttcag acctgctgga atatgcagac
     6181 tcagcagcgc ggttgccggc agaagatgcc gccggctgat atatccccag cgcatataca
     6241 gcacacacag caccgtcaga taaaagccga taagcaaagc ccagagccaa tgggcatcgg
     6301 ttttgtgcgg cgatttgtaa aacagataaa acccaatccc cgccggtaca gcgaaatagt
     6361 aaagaaagtt gatgatgatt ttattgacgg cctcagcaag ccctttggac aagtcggccg
     6421 gattgaatcc ggcggtttcg acggcattgg acgcagattc agtaagcagt atagactcac
     6481 cgaaagccgc agaagggctt ttaagcagct ggttaagctt atgggggaca acctgttttg
     6541 tcagcagtgc atacggcaga aaaaccatca aaaaacctgc tgcaagccat atatagcagc
     6601 cgttttgttg gaggggtttc ttttccataa caccgcgcag ggttttatat gcaaaaaaga
     6661 acagcccata cagcagcacc tgagcgcatt ccggccggat aagagtaccc agcccgcagg
     6721 acagccccga gaccccaaga agcacgccgt tgagagtttg acttccttgg caaaacagca
     6781 gcagactggt gcttagaaac agaagaaaag gccactcccg caagacatcc gcacctgctt
     6841 gggcaggata gggaagcaca atcagtgcca gcatcgcctg caccgcgtgc tgccgtccaa
     6901 aaagccggct ggcccagagg tataacacgg cgattgttaa aatgcggcat cccagcacgc
     6961 acacctgtcc ggccagacac cacaaagccg gcgagtcgcc gccagtcagc tgcagaaaaa
     7021 atccatggca taaaaagata agcaccggca aaccaatggg ttcatgccgg ctggcaacat
     7081 tctggtaatt gtccggcaac tggcgggccc gttcgatata cactaccccg tcttcggaaa
     7141 taacggttgt gcgggcaatc agaaaggcgc ccagcacggc tgccgccgct gccagcagta
     7201 tcatatcccc cattttctgt ctgttgtctg aaaaggtctt cattgcaatt tggcggccga
     7261 atgcgcttct gttttggttc ggctgaaaat actgttgttt ttttgcaggc aaagtttacc
     7321 atatagaccg gtaatgccaa ctaaaaactt gtgccgaaag cggagaagat gactctgtat
     7381 cctgctgatt atataatact tgctgccatc gtgatgcagg ggttgtttac gattcaggtc
     7441 gtaaataact gccgctatgc ccttgaaaag ataaaacgcc gccgggaatt gtaccggccg
     7501 cgctgtgtgc tgattgtgcc atgcagagga ctggatgagc attttgatga gaatatcgag
     7561 tccttctaca ggcaggatta tgagggctat catctgtggt ttgtcgtgca ggaccgcagc
     7621 gatccggcct atgagcggct gcttcggctc aaggcccgcc acaggcagaa cacacaggca
     7681 cagtccgtca aaatccttgt cgctggacac accgactcct gcagccaaaa actgcacaac
     7741 ctgctgtatg cctgccggca aataccgccg gagaccgaag cccttgtttt tgccgattcc
     7801 gatgcctgtg ccggtcccca ttggctgtcc catatcgtct atccgctgcg aaaggacaaa
     7861 aacggcgccg ccggcggcta ccgctgcttt gtaccccaga caaacaacct tgcaaccgtg
     7921 gccctgtcgg cggtcaacgc caaaatctgc cagcttctcg gcaatacccg cttcaatctg
     7981 gcttggggcg gctccatggc gattttggtc aagaccttca aagaattggg aatcgacaaa
     8041 gtctgggaaa aagccctcag cgacgacctg accctgaccc gggcgattcg caaaaaaggc
     8101 ctcaaaatgg tttttgtccc cgcctgtatg attgcctcgt atgaaagcgc cacatgggcc
     8161 agcttgtggg aatttgcccg caggcagttt atcatcacac gcatttatac tcccggcatg
     8221 tggctgttcg gcttgttcag tgccttgttt acggtagggg ggctgtgggg aggtctggcg
     8281 gcggctgtct gggctgttca aacggattat ccccacagcg gtctggttat cgcgcttcct
     8341 gcggtgttct tggcctgtca gcttctaagg gcgattctcc gccagcgatt gatagccaaa
     8401 ctcctgccgg ccgataagga gcggctccgc atcgcccgct gggcggatat cctgtttttc
     8461 tggctctggt ctatcctgct gctggcagtc atcctctcgt cggcaatagg ccgcacgctg
     8521 gtgtggcgga atatccgcta ccggattgaa tctcctgtct gtgtgcacat cctcgggcgg
     8581 tcgtgaatgc cgcccgctgc cggcggcgtc tatagcgtcc ggtccacggc ttcggcaacc
     8641 cgcctgagtt tcggcattag ttggctcagc gtctgggggg tgatggactg cgccccgtcc
     8701 gatgcggcat gttcggggtc cggatggcac tccacaatca gcccgtcggc gccggcggca
     8761 atagccgcca gacacatggg aggaaccata tacgcatgcc ccgtgccgtg cgacggatcc
     8821 acgataaccg gcaggtgcgt ccgttcccgc agcgccggga taatcgccag cggcagggta
     8881 ttgcggacat attcctcaaa tgtgcggatg ccgcgctcgc agagaatgac gtttggattg
     8941 ccggcattga tgatgtattc ggccgccagc aggaattcat cgatcgaggc gctcatcccc
     9001 cgcttgagca gcaccggctt ttgaaccttg cctaccgcct ccagcagcgg ataatgctgc
     9061 atattgcggg cgccgatctg cagcacatcg ctgtactgag caaccggctc cagctgttcc
     9121 agaccaatca cttctgtgac gatggccaga cctgtttctt cccgcgcttc ggccagaatc
     9181 ttcagccctt ccagacccag tccttggaag ctgtagggac tggtgcgggg tttgtacgcg
     9241 ccgccgcgca ggccgatgca tccggcctga cggacggctt gggcggtggt cataatctgc
     9301 cggcggtctt caaccgagca gggccctgca ataatcccga cagcggggcc gccgattgga
     9361 aaccccttcg gtccaatcgg gatttgcgtg cgctcctttt tgacctccag agaggccatc
     9421 ttgtagggcg acagaatagg cacaatcttt tccaccatcg gggcattttc gatggcgtcc
     9481 ttattgacag cccgcttgtc cccgaggcag gcgataaccg tccggtcggt gccgtagatc
     9541 gggtgttctt tgagaccgta gtcattcacc agcgcgataa catgctgtat ctgctccttg
     9601 gttgcgccgg gcttcattac aataatcatg tcggtttcct ttactctgta aatccacagg
     9661 ttgttttagg ttaattatag caccagccgc accgaattac aaccggcttt ggatttgaca
     9721 aaagggccgg ggtctgtaca atgatcggac agatgaatcc tgcgacaata aaactgctgg
     9781 tgctggccga tatccacggg cggcatgaga aaattgcctc gctgcgtcct ctcgtgaagg
     9841 atgccgatgc cgtcatactg gccggcgata ttacccattt cggcggggcc aatgagactg
     9901 ccgaagtgct gacggcactg gagccgctgg gcaagccggt ttttgccgtc cacggcaact
     9961 gcgaccagcc ggtagtgcag gaggttttga gccggcgggg catttccgtc cacggctgca
    10021 caaaagagct catggggctg tattttgccg gtgcaggcgg ctctctgggc tggtcggggg
    10081 caacgcccgg cgaggtgggg gaagcggcct ttgcggcttc gctgcagcag gcggctgcgg
    10141 ggctgaatgc cccaagccgg ctggtgctga ttacccatca gccgccgtgg gggactactc
    10201 tggatgtgca gggcggcatg cgccataccg gcagccggac gattcggtcg tttatcgagg
    10261 accatcagcc gcttctggcc gtcagcggtc atatccacga agcccgcggc atcgaccggc
    10321 ttggcgacac cgtactggtt aatcccggtc cgctgcggag cggctgttat gcggcggtcg
    10381 aaataaccaa ttgcgatgtg caggtccggc tgggtctgct ggactctgct taaccggaac
    10441 agagggcagg gatgaaccat tttgagcagc ttcatccggt tgtgcaggcc tttctggcaa
    10501 cgctgtttac atgggcactg acggccctgg gggcggcatc ggtttttctg accaaaacca
    10561 tcagccggcg tctgctggat tttatgctgg cctttgccgg cggggtgatg attgccgcaa
    10621 gtttctggtc gctgctggcg ccggggattg aaatggctca gcttcagggg cagactgcct
    10681 ggattccggc ggctgtggga tttctcctgg gcggggcatt cctgcggctg atggaccgat
    10741 ttctgcctca tctgcatctg ggatatccaa tgtcggaagc cgaaggcgtt catacccgct
    10801 ggcgccgcac aacgctgctg gtgatggcga ttactttgca taacatcccc gaaggattgg
    10861 ctgtgggcgt ggcgtttggg gcgctccgat acggcctgtc gtcggccagc tttactgccg
    10921 cctgtgcact ggcaatgggc atcggcctgc agaatctgcc cgaaggaatg gcagtatcgg
    10981 ttcccattcg ccaaatgggc accagccggc tgaagagttt ctggtacggc cagctgtcgg
    11041 ggatggtcga accgatagcc ggccttatcg gtgcggccgc agtagtgctg gtttcgccga
    11101 ttcttccgta tgcgatgggt tttgccgccg gcgcgatgat ttatgtggtg gtcgaggaag
    11161 tcattcccga atcccagcga gccggcaata ccgacttggc cacggtggga acgatggtcg
    11221 gctttgccgt gatgatgacg ctggatgtcg cattgggctg actgtccctg cgcatccagc
    11281 ctgccgaagg ccttctgtgc tgcctttctc ggacaggcat cccagaacgg ctcaaaggct
    11341 gtcctgacag acacacctgt cggccgctgg aggccgacgg ctttcttttt cagtgtacgt
    11401 ttctatttac ttcttttaaa ttatggtagc aaaaacctgc atagtaactg cagaaaacct
    11461 tcgcagtaga tgcagaaaac ctgcccagta agtgcgtaaa tctgcgtggt cagcccacta
    11521 gaacagccgg caagctggtt tgaactgccg ccgccggctg caattttgct aactgcttat
    11581 ccggaaagca gataaaaatt tagagtacgt tcaagacagc agaatagtgc atacagaaaa
    11641 aaataactgg acattgtagg tttacaatta tagagacaat ctcaaaatgg tatcggataa
    11701 gcagtgtcgc gatacagcag cactgaaggc actgcttgag ctgtagttga agcaggcaat
    11761 aagattcaga agaaaatcgc agcggttaat acagcaggcc ggccagccag ttttccgcca
    11821 ataccgataa atccgccaaa tcgaccgctc cgtcgggggc gttcagcggg gccaaatcgg
    11881 cgctggtatt ccagaccgta agaccgcttc cctgccaagc gctgctgaag gcagccaggt
    11941 cctgcatatc gaccttgccg tccagcggca ggggggcgat gtcggctgtc tgtcccgctg
    12001 cggcaatccg ctggaattcc agacgggggt cgtcttcgag cataaagccg ccggcacgca
    12061 gtatcaggtt gtactgagta aagaggttca tcccgataat gccgtccagc gtgccgcctt
    12121 cggggctgaa aatatccagc agcaccaccg gcacattggt aaactccagc cattggccga
    12181 cggccggaat cgtcagccgg tccaagtaaa atcccggggc cataatcgaa tcgcccgtaa
    12241 cgccttcgat ttcaacttca aagtccctgc cggctgcctg cagtccaagc cgtgcagcta
    12301 tccgcgagcc gataacggta atctgggcgc cggtatcgag cataaagcgg tttttgtcca
    12361 ctgcactgcg gccgctgtcg gtcaaatcca ctccgtgaat gaaaaacaag ctctgcgagg
    12421 aaactcccat caccaccgac ggcgtcatcg gcgaataatc catttcaaag ccccacgggt
    12481 ctgtgaacat ctccagcaga tcttcgggat tgaagccgta ggtaatatac tgcactgttg
    12541 tgctgccggc gggcttgagt tccagcggaa tataatcggc ataggccgga ctgggcgagc
    12601 ccttgggata gaaggtaatc cgcgggccgg tatagaccgt ttggctgcgg gttatggtga
    12661 tgggatgatc gacctcgata tgcgtatcgt agaagacgct catcggcgtg ccgacggcgg
    12721 tagccaggtc gggtttggtg cccgggtttt gaccaatcag cgtggcgaca ttgtattggc
    12781 ccagcaggcc ggccgttgag ggcagaatca tttcactctg gccgggggcg ttgggttcca
    12841 ggacatccaa gccggccata aagagggcat acggccatgt tatccgcgct tcaacggagc
    12901 cggttacgcc ggttacctcg gctttgtttt tctgggtcag ataggtgctg ttgtacagtc
    12961 ctgcacggac ggcattgtaa tagccgatga catgggagct ggcgccggta tcgaagatgg
    13021 caatcataaa gtccgtccgc ggattcgttc ctgccggcgg gcttccggcg taggaggacg
    13081 gaaaggcctc gtaaatgcct gtcgtttcgg catccagatg cttattggtg ggcgtcagca
    13141 caatccacgg cacaaagccg tccaccggcg gcgaatcgat gacgcccgat accgctgctg
    13201 acggagccgg tgctgcaagg gcttgtgctg cggccggctc tttgtgaaaa taccggattt
    13261 gctttatcct tgaattggaa ggtccttgcc agttgacggc ctgaccgtcg gcccgtactg
    13321 aaaggccggc acatgctatt acagcgctga cgaaagagaa taaggacgca cacttacgca
    13381 tggtagtaat cctttcctga cttagaacat ttgttcttat ttcggtcaaa tcaagccccc
    13441 gcaaaacgaa agaagcacaa aaatgctctt tgctgtttat tataggcaaa ccgtccgata
    13501 aaaacaagaa gatttgaggc cgcaggctgg cgggcgttgg gatttcggac tttcctctgc
    13561 ggctgaacgc gaaaagggca ttttggaggc tgccggcctg cggtaaagag agccgcaggg
    13621 cagaaaatat gcccgaattt tcataaaccg ccttcccttt tgagccgata gcaactgcag
    13681 aaacagcggt ttgcattcag gagataagat tatgggtatt cagaactggt cggaaaatgt
    13741 aatattggtc aacttgtgca aagaaccgga tttgggcgag gaactgcaga cggttatcga
    13801 gatggtcggc gcggatacag cttcggatgt agtgctggat tttgcggata ttgacattat
    13861 cacttcctcc agcattgcca agatgctcaa gctccgcaag gttctgcgcg acaacgggcg
    13921 gacgctgctg ttttcgtcgg tcggcccgaa gaccaaatca attttcaatg taaccggact
    13981 ggatactgta tttgaatttg tcgatgacca gtttacagcg ctggcctcat tgcagatggt
    14041 caactgaggc gcaggggaaa aagacagccg ccggacagac cgtacttgac agtctgtccg
    14101 gttttgcttt atagtgcacc aaaaacaaac ggcaggcata ttccgaacgc cgctggcgtc
    14161 agcggccttc gcaatctgct tgcctgacag ccgtcaggag accattatgg atgcacaaac
    14221 tgtccgccgg attgaagagg cgctgggcta ccaattcaaa aatccgcagc ttctggccga
    14281 agcatttctg catgcctcgg ctgccgacga ccgccgccgc agcaacgagc gtctggagtt
    14341 tctgggcgat gcggtgctgt cgctggtggt ctgccagacg ctgtttgagc gttttcccga
    14401 ctacctcgaa ggagatctga ccaagatcaa aagccgtctg gtgtcgcgca aggtctgtgc
    14461 tgaactggcc caccagctgg ggctgccggg gcatctgcgg ctgggacggg gaatggaaag
    14521 cagccgcgcg ctggccggct cgattgcggc agccagtctg gagtcggcta tcgccgccat
    14581 ctatctggac ggcggaatgg aagcggccaa gacctttatc tgccggctgt atgagccgct
    14641 gtttgtggag gccgatgccc agcagcatca ggaaaactac aaatcgctgc tgcagcagta
    14701 ctgccagcag gaatacggct gcatgccgat ttatgacctg ctggacgaga aaggccccga
    14761 ccacaacaaa tgctttgaga ttgctgttgt ggtgcggcat ctgcggtttg attcggcatg
    14821 ggggattacc aaaaaagaag ccgaacagaa agcggcctac aatgcgctgt gtcagctggg
    14881 gctgatgggc ggtccggcgg ccggcagcga ttagccggtt atcagccagc tgagcagatt
    14941 cagcacaaca atcgccagaa tcataaagcc gctgacggtg gcaaacagca cggccgattc
    15001 gaccagaaac cgccgccaca gcagggcccg catcttggtg gccttataca ctattgcaat
    15061 ggctgccagc agcggaaaca tccacagcag cgatcccggt gtggttccta tctgctcggg
    15121 ggcggtaaag gccgctacaa tccactcatt cattgacgcc gtcctcctcc tgaccaatca
    15181 gttcaccgcg tccgcagtag gccatataca cggcgcttaa gatgctcagc aggttcagca
    15241 gtcctgcaat gccggtgtat atttggccga tatcgcaggg gcggccgtag gtgggaaagc
    15301 gaccgttctt ggtgaagctg cccagtatcc cgac
//
LOCUS       DAOTBO010000070        15210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3274_length_15210_cov_4.971297, whole genome shotgun sequence.
ACCESSION   DAOTBO010000070 DAOTBO010000000
VERSION     DAOTBO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 15210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15210
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3274_length_15210_cov_4.971297"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2510
                     /locus_tag="PLE88_08775"
     CDS             <1..2510
                     /locus_tag="PLE88_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOM76594.1"
                     /translation="KTPFDVMIPDEAARVRAVFERCVSEAKPFSGLENICLHRDGRQV
                     YLETSGVPVFDPQGRLVGYRGVDRDITERRRIRQMLETYQAEISSIFSASPIGIGLVR
                     QRRLIHGNERFFDLSGYSAEDLLGQDPRKLYLSQADYAEVGRLYVEALSGGIAAIETR
                     WRRKDGRIIDVQMHIAPLDSPDAEKGFVIMAHDITAAKAARQALKDNEAFLRLIFDGI
                     QDGLCVLDTQFNIVRANVYQQQRHCRKGSQIGRKCYEVYHGLSAPCAGCPSLKAMETG
                     QTQSAVVPMTEAGRPTGWLEINAFPMQDASGAVTGVIEHVRDITQRRQIEEALEENRR
                     AQTTLMGNLPGAAFRCLDQPDWPMQFISKGCLELTGYTDQEFICQHVVWRKLIVPADR
                     ARVWEEIRQALKNRTNYQIVYRIRTKDGQEKWMWEKGCGVYDADGEVAAIEGFITDIT
                     ERQKIREALMFTQFSVDHAGEAMFWMTHKGRVVYANDAACRSLGLDRSRLLEMSVWDF
                     APSLTPQRWQQHWQELKEKQTLRFESQHRRSNGSVFPVEVTANFLVCEDAEYNCAIVR
                     DITERRCAEQTRQLLLQQLQTRNEELQSIVFIAAHDLRSPLVNIAGFAGELEKDLARL
                     TAVLQETAGSLPPQADTLLRRELPESLRFIKAGSSKMQALLDGLSRLSRIGTLQINPQ
                     QLDMAALMQSAVADLNFQITACGAQVDIEPNLPPCRGDAVLIRQVFANLVDNAVKYRR
                     PDRPCRIRIAGRSCGDGRVEYAVSDNGIGIPPEHIDKVFELFHQLCPDRSNGGQGLGL
                     PIVRRILDRQDGSVRMESQPDVGTTVYVQLPAAGQEQ"
     gene            complement(2548..2955)
                     /locus_tag="PLE88_08780"
     CDS             complement(2548..2955)
                     /locus_tag="PLE88_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOM76595.1"
                     /translation="MKTIAWDNSFETGNTVIDQHNKAYLCRLNRLIKAIEAHPRQAAA
                     LLGDFLPYARRHFRAQESSLARLVDAAAFEDHQQRHRFFEDFLSRPLLDADGGAEEIC
                     AFMADWTAFHIQHLDKDALSGLSASSEAAGSRV"
     gene            complement(3135..3722)
                     /locus_tag="PLE88_08785"
     CDS             complement(3135..3722)
                     /locus_tag="PLE88_08785"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOM76596.1"
                     /translation="MADYTLKQLTQQPWLNLYEASYAAPDGRRHRWLMCSRKQRPIAD
                     AACPDAVVIVPVLPTQQGRRLVLTSEFRIALNDWEYGFPAGLIDPGESIEQTVRRELK
                     EETGLDTACIHHLSMPVYSSAGLTDESCIMALVTAAGVPSTALNAPGEQIRVHLLDVP
                     DIRALLAANKKIAAKAWGLLYHFAACGQIAFADGL"
     gene            complement(3733..4185)
                     /locus_tag="PLE88_08790"
     CDS             complement(3733..4185)
                     /locus_tag="PLE88_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOM76597.1"
                     /translation="MIEFRTFEDILDFAILQEKAAQQFYTHLAGLVLDPDVQLFYRTL
                     AEEERLHEKKLLELKRYEFDLKEPDLESLRSSGYLDAMPAHPEMTIAEAVRYALKKEK
                     SARMLYHTLARMTHRPELAGLLEQLAQQEQQHADFFLEQSERHPLDSK"
     gene            complement(4541..4822)
                     /locus_tag="PLE88_08795"
     CDS             complement(4541..4822)
                     /locus_tag="PLE88_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76598.1"
                     /translation="MYIERLSDPEYNLNLDNWYFHLEPGDPGGHWDSGVYWLPIPYNP
                     EWVSVDVQGAGNVGIFNGVFWHECIVPEPATLLLLAVGGVGMMGIRRKL"
     gene            complement(5000..5227)
                     /locus_tag="PLE88_08800"
     CDS             complement(5000..5227)
                     /locus_tag="PLE88_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76599.1"
                     /translation="MKKTDVFGIAIAVLLTAVGAAEVREGYFQVDLIGPDELVWDPAL
                     NSGFEGQWYRYPQPDVAPQEDPQPVFPWWNE"
     gene            complement(5654..6916)
                     /locus_tag="PLE88_08805"
     CDS             complement(5654..6916)
                     /locus_tag="PLE88_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76600.1"
                     /translation="MKTTQLICLLAVLTALSSSVCKAMYFAEGSIAEITAAPDHQVYG
                     REQSFAVCGSTAVWLDYRDPMWIPRVYGVRLDDPNFQEFAIDTNAADIQTLAFCGHRA
                     VYPVWPLDKQYLRVADISDPGSPVLADIGLQIPSLLYFDYAGGLIAYAGDDQLNSWRD
                     TIYAVDASELNNPQTYTVYVEPDEVTVYGLTAEAGSILWYITNYNELTRSVRIADVSN
                     PQEPVIRTAVLPGDYDRYWTHFEVSGSWLVASEGWPYRILGVHNYQDTDNWQIQVLWQ
                     EGQDGEQLVSGPRIDGPIAVWITTTRMPALAQMNGTAGLYQYRLKAAYLMDNGHFTVW
                     TLLESETDELLAADISGRQVVWSVWNRQTELIDLFKGTLELQCGDWGYKAGDLDKNCF
                     VNLLDLVLMAQDWLACTDPQQPECGFGL"
     gene            7467..8876
                     /locus_tag="PLE88_08810"
     CDS             7467..8876
                     /locus_tag="PLE88_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOM76601.1"
                     /translation="MGFDLKIFNDASLEPSYVEWLVEQRWPQAAQYFGRLWDYYTNPL
                     TETDRSSESSRGYMQAQETGLPLRITGQPAREGGQIQRKEVVIENDIGWRINAMVDFL
                     FGRPVSIKSKAAAAHRRRDIEGVLKAVFDGSGGTVFFQELGTLGSVYGFVDCIVRWGG
                     QLAGRLADSTSNRLLCVPPSSYDSVLRLAGDVSLELIDAARALPVLEETDYKRIRYYV
                     QHFVQWKNAAAGESSFLGRLLGTGALGQRRCAAVTEILGPSAWQRYEDGILTAQGVHP
                     LGRIPVVHIQNLPQPGCYEGLSDVQQLCPLQDELNTRLSDRASRITMQAFKMYLAKGL
                     DGIAERPIAPGRMWCTDNMDAAIEEFGGDGGNPSENQHIQEVREAMDKVSGVTPVVAG
                     VLKNKLGNLSSAVALKMTFMGMLSKTCRKQLTYGQGISDICRLVLEVLDKAAVFPTRP
                     AERQVEVVFSSPLDEQINR"
     gene            complement(8975..9265)
                     /locus_tag="PLE88_08815"
     CDS             complement(8975..9265)
                     /locus_tag="PLE88_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76602.1"
                     /translation="MNAAENTLCAYANQCSFYQQSRTYMPAVSQPLRTEFCQTDNSRC
                     ACRQILSQLGIEWLPLHMMPYQYEWAQQILDDIANSNVPLEAALNPDTLQTF"
     gene            9606..10139
                     /locus_tag="PLE88_08820"
     CDS             9606..10139
                     /locus_tag="PLE88_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76603.1"
                     /translation="MFVSKKQKSSSRHSRQQRFCEVLYRTTDITLADAMSQCGISPKT
                     FADWMDDSSLHAALQLQFRIKLMEGRSIIGRAVPMAAAKLVELLCKDSAQAAGSDKNR
                     DEAIRKTCLDILSLGNAYEQSIACQDKPDTAPLLSDEEAEKLIRALTEDEDDPDDGDG
                     QDNGPLYNQAKGDTEPL"
     gene            10258..11487
                     /locus_tag="PLE88_08825"
     CDS             10258..11487
                     /locus_tag="PLE88_08825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOM76604.1"
                     /translation="METAADHIEAVLEQAAVSSELKERALEATAEGVVIADCTQEGMP
                     IIYVNSGFTAMTGYAAQDVLGRNCRFLQGADSDPQAKETIRQAIARRQPCSVEILNYR
                     KDGTPFWNRLSITPVRDAAGRVTHFIGIQTDITVRRNAEDLLRAAKEQLEQANALLHR
                     DLEAAAAIQQTLLPAELPEAAGYRFGWRYRPCRQLGGDTLNITRLDKRHIGLYVLDVS
                     GHGTAAALQSFSLSQTLGRRREYSFLYRQSGRRHRVCPPQEVLRRLNRSFPMDMQTGI
                     YFTMLYGLLDTQTGVFAFASAGHPGPVLVRAGSRPEILDADGFPIGVVEEADYRLQKV
                     QLCSGDKLILYTDGVVEALSAREAAFGRERFLRALVKTSGQPIEACLDAVMTALEHWA
                     CHNNLRDDVSLAGLERI"
     gene            complement(11563..12021)
                     /locus_tag="PLE88_08830"
     CDS             complement(11563..12021)
                     /locus_tag="PLE88_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76605.1"
                     /translation="MSAKYLVVLVAAAACWLLAGCQPSYSAEMSIPLSAADRQTLPIQ
                     PLDVQAAVEKIAAAAGLVPYTARAEEEDLLSIADSEQLNPAAPADSARLWKHPEQPVF
                     LSLTRHPGEFVVLANYARAGKTDPKAVKVYKALEKQLSALPASLTAGLKL"
     gene            complement(12118..12300)
                     /locus_tag="PLE88_08835"
     CDS             complement(12118..12300)
                     /locus_tag="PLE88_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76606.1"
                     /translation="MEKKGQADLIVMAALFVSGLLVIGSAVDAFSKDAGSVFVGSSVL
                     FAAALLCRAMQWASKS"
     gene            12401..12706
                     /locus_tag="PLE88_08840"
     CDS             12401..12706
                     /locus_tag="PLE88_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76607.1"
                     /translation="MTDTSQPTAVNPEQPDTPDSGQLNRLREEIESLKADLRALQAAA
                     AGGEAPGAGGIQPKTQGVRESLGAGRRAALERAARAAARSGSRIDVQEYMRIRRSFV"
     gene            12786..13940
                     /locus_tag="PLE88_08845"
     CDS             12786..13940
                     /locus_tag="PLE88_08845"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HOM76608.1"
                     /translation="MSFSGKATYSAGVNLPELAEDVSDVISIVSPYETPLLDALGDPV
                     RAATSTHHEWLEDALLPNKDVIADTTIGDPETETEFDVAHPDRFRPGDQIQPQGSGEL
                     MLVTAVSGSTLTVMRSYAGTVADAIAPGQTLCILGNAALEGGDKPAARFTTRQRCGNY
                     TQIFTAAVEVSGTDLAASQLGLADEMDYQKQERLRELLRDLENTVINGGAPTADPQGS
                     TSVRRTMRGILAHLVSHVFMPGVNGFPSGATLDEDKLNWALRKIWEDSSGTVDMIVVG
                     GLQKRRINGFLSALRGYASGDTVYRDGVSAYESDFGLCRIITTRWMPADAVLLLDSSR
                     VKVLPLAGRSFHFKPLASGGDYECGQLIGEYTLELRNESAHGVIRGLAVS"
     gene            complement(14017..14748)
                     /locus_tag="PLE88_08850"
     CDS             complement(14017..14748)
                     /locus_tag="PLE88_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOM76609.1"
                     /translation="MHCLFCILLTVRLGCALPAGAEKTVDYPDPNRWAGEMKTFADWD
                     AKNTPPQNAVVFVGSSSIRLWKTAEAFSGLPVINRGFGGSWMADSVCFADRLILRYKP
                     AVVVIYAGDNDIAGGLPPEQVHRDFVRLTQIIHAELPRTPIICLPVKPSRSRWTLWPK
                     MQQANQLNRQYAQTAGYILYVDTASVLLGGDGLPDETLFEPDALHLNQKGYDQWNRLL
                     RPVLNRLYPADAPPAAQLHEAPAQE"
     gene            14847..>15210
                     /locus_tag="PLE88_08855"
     CDS             14847..>15210
                     /locus_tag="PLE88_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76610.1"
                     /translation="MSGFSSDADLARYEPGVFDRWILNGQVLCRGTNGLLAGTQFAAG
                     GADFTAAQVKAGHILWLQSTDGSIAGAYEIVQVPDSGHLTVSVLRADPQQPAIPAGAA
                     SGLLWRIVSYDVQAYEVQC"
BASE COUNT         3283 a         4055 c         4706 g         3166 t
ORIGIN      
        1 gcaaaacgcc gtttgatgtg atgatccccg acgaagcggc acgggtgcgg gcggtatttg
       61 agcggtgtgt ttcggaagcc aaacccttca gcgggcttga aaacatctgt ctgcaccgcg
      121 acggccggca ggtgtatctg gagaccagcg gcgtgccggt gtttgacccg cagggccggc
      181 ttgtcggcta ccgcggggtg gatcgggata tcaccgaacg cagacgaatc cggcaaatgc
      241 tggagactta tcaggccgaa atcagcagca ttttcagcgc ctctcccatc ggcatcgggc
      301 tggtccgcca gcgccggctg atacacggca atgagcgctt ctttgatctg tccggctaca
      361 gcgctgagga cctgctgggg caggacccgc ggaaactgta tctgtcgcag gcagactacg
      421 ccgaggtcgg ccggctgtat gtcgaggcgc tgtccggcgg gattgcggcg atagaaaccc
      481 gctggcgccg caaggacgga cggattatcg atgtccagat gcatatcgcc ccgctggatt
      541 caccggatgc cgagaaaggc tttgtgatta tggcgcatga tattaccgcc gccaaggccg
      601 cccgtcaggc gctgaaggac aatgaggcgt ttctgcgttt gatttttgac ggcattcagg
      661 acgggctgtg cgtgctggat acccagttca atatcgtgcg ggccaatgtc tatcagcagc
      721 agcggcactg ccggaaaggc agccagatag ggcgcaaatg ctatgaggtc taccacggcc
      781 tgtcggcgcc gtgtgcgggg tgtccgtcgc tgaaggcaat ggaaaccgga cagacccagt
      841 cggctgtggt gccgatgaca gaggccggcc ggccgaccgg ctggctggag atcaacgcct
      901 ttccgatgca ggatgccagc ggcgctgtaa ccggcgtcat cgagcatgtg cgggatatca
      961 cccagcgccg ccagatcgag gaggcgctgg aagaaaaccg gcgtgcgcag acgacgctga
     1021 tgggcaatct gcccggggcg gcgtttcgct gcttggacca gccggactgg ccgatgcagt
     1081 ttatcagcaa gggctgtctg gagctgaccg gctataccga tcaggagttt atctgccagc
     1141 atgttgtctg gcggaagctg attgttccgg ccgaccgcgc gcgggtctgg gaggaaatcc
     1201 ggcaggcgct gaagaaccgc accaattacc agattgtcta tcgcatccgc accaaagacg
     1261 ggcaggaaaa atggatgtgg gagaagggct gcggcgtgta cgatgccgac ggagaagttg
     1321 ccgccatcga aggatttatt accgacatta ccgagcgcca gaaaatcaga gaggcgctga
     1381 tgtttacgca gttttcagtg gaccatgccg gcgaggcgat gttctggatg acgcacaagg
     1441 gcagggtggt ctatgccaac gatgcggcct gccggtcgct gggtttagac cgcagccggc
     1501 tgctggagat gtcggtatgg gattttgccc cgtcgcttac gccccagcgg tggcagcagc
     1561 actggcagga gctgaaggaa aagcagaccc tgcggtttga atcgcagcac cggcgcagca
     1621 acggcagtgt ttttccggtt gaggtaacgg ccaattttct ggtctgtgag gacgccgaat
     1681 acaactgtgc gattgtgcgc gacatcacgg agcggcgctg tgccgagcag acccgccagc
     1741 ttctgctgca gcagcttcag acgcgcaacg aggagctgca gagcattgtg ttcattgccg
     1801 cccacgacct gcgcagtccg ctggtcaaca ttgccggttt tgccggcgag ctggaaaagg
     1861 accttgcccg gctgacggcg gtgctgcagg agacggcggg gtccctgccg ccgcaggcgg
     1921 acacactgct gcggcgggag ctgcccgaat cgctgcggtt tatcaaggcc ggcagcagca
     1981 agatgcaggc cctgctggat gggctgtcgc ggctgagccg catcggcacg ctgcagatca
     2041 atccccagca gctggatatg gctgccctga tgcagtcagc cgttgccgac ctgaattttc
     2101 aaatcaccgc ctgcggggca caggtggata tcgagccgaa cctgccgccc tgccgcggcg
     2161 atgcggtgct gattcggcag gtgtttgcca atctggtgga caacgccgtc aaataccgcc
     2221 gcccggatcg gccgtgccgg attcgcattg ccggccgctc ctgcggcgac ggccgggtgg
     2281 agtatgccgt cagcgacaac ggcatcggca ttccgccgga gcacatcgac aaggtctttg
     2341 agctgtttca ccagctctgt cccgaccgct ccaacggcgg acagggactg gggctgccga
     2401 ttgtgcggcg gattctggac cgccaagacg gcagtgtgcg gatggaatcg cagccggatg
     2461 tcggcacgac cgtctatgtg cagcttccgg ctgccggcca agagcaataa ggcggcggct
     2521 gccgctgtgc gcaaggtctg tgcggcatta gaccctgctg cctgcggctt cgctgctggc
     2581 cgacaagccc gacaaggcat ctttatccag atgctgaatg tggaaagccg tccaatcggc
     2641 cataaaagcg caaatctcct cggccccgcc gtcagcatcc agaagcggcc ggctcaggaa
     2701 atcctcaaaa aaccgatgcc gctgctgatg gtcctcaaat gccgctgcat cgactagccg
     2761 cgccaaagaa ctctcttgag cccggaaatg ccggcgggca tacggaagaa aatcccccag
     2821 taaggctgca gcttggcgtg gatgtgcttc gatggctttg attaacctgt ttagccggca
     2881 gaggtaggcc ttgttgtgct ggtcgatgac agtgttccca gtctcaaacg aattgtccca
     2941 tgcaatagtt ttcattgttt ctactgcctg caaatcatgg aacatatgta tcttatatcg
     3001 gtatatttac tgatgattga ttaggttttt tttcataatt ttcctaaata ttgtgaaaaa
     3061 ggtgcgaaaa ggcaatttat cggacgtctc aagagggggt gtttgccgac tgcttgaaaa
     3121 gctcacagtg ccgcttaaag gccgtcggca aaggcgattt gtccgcaggc ggcaaagtgg
     3181 tacagcaggc cccatgcctt ggcggcgatt ttcttgttgg cagccagcag cgcccggatg
     3241 tcgggcacat ccagcagatg gacccgtatc tgttcgcccg gtgcattcag ggcggttgaa
     3301 ggcacgccgg cggcggttac cagcgccata atgcaggatt catcggttaa tccggccgag
     3361 gagtaaacag gcatggagag gtgatgaatg caggccgtat cgagaccggt ctcttctttc
     3421 agttcccgcc ggactgtctg ctcgatggat tcgccggggt caatcagtcc ggccggaaag
     3481 ccgtattccc agtcgttgag ggcgatgcgg aattcgcttg tcagcaccag ccgccggccc
     3541 tgctgcgtcg gcagaaccgg cacgatgacc accgcatcgg ggcaggcggc atcagcaatc
     3601 ggccgctgct tgcgcgaaca catcagccag cggtgccggc ggccgtccgg ggcggcatac
     3661 gaggcctcgt acaggttcag ccacggttgt tgagtcagct gcttgagcgt gtaatctgcc
     3721 atagggggag gcctatttgc tgtcgagggg gtgccgttcg gactgctcca gaaagaagtc
     3781 ggcgtgctgc tgctcctgct gggccagctg ctcgagcaga ccggccagct ccggccggtg
     3841 cgtcatacgg gctaaggtgt gatagagcat gcgggcggac ttttcctttt tgagggcata
     3901 acggaccgcc tcggcaatgg tcatttcggg gtgagcgggc atcgcatcca gatagccgct
     3961 ggagcggagc gattccaagt ccggctcttt gaggtcaaat tcgtagcgtt tcagctcgag
     4021 cagttttttt tcgtgaagcc gctcctcctc ggccagcgtg cggtaaaaca gctgcacatc
     4081 ggggtcgaga accagtccgg ccaagtgggt gtaaaactgc tgggcggctt tttcctgcaa
     4141 aatcgcaaaa tccagtatat cttcaaaagt gcgaaactct atcataacgg cctccgatgt
     4201 tttgggtttt tctcatcggc attatacacc ccggccggca gcccgtaaac aagaaagcgt
     4261 cgaacggcaa aaaacgcaaa accggatgcg tttattgcag attgtccccg ccgataaaaa
     4321 aagccgcaag gggttgcccc gcggcctcta tagaatagag gtgatgaggt tttcatcacc
     4381 ctctccacaa cataatggta ctattatact ttttcaccag ttccaagtca aggacaattt
     4441 atcggctgct gctgccgaag gccttacagc agcagttttt taataaaagc ctgacggcac
     4501 cgcatacgca cggccgatgc cgtcgggcac tgctgatggg ttagagcttt ctgcggatac
     4561 ccatcatccc cactcctccg accgccagaa gcagcagggt tgccggttcg ggcacgatgc
     4621 attcatgcca aaacactccg ttgaagatgc cgacattgcc ggccccctgc acatcgaccg
     4681 agacccattc cgggttatag ggaatcggca gccaatagac gccgctgtcc cagtggccgc
     4741 ccggatcgcc cggttcgaga tggaaatacc aattatccag attgaggttg tactccgggt
     4801 cgctgagccg ctcgatgtac atttccggat tgtaggccgg cgggtccgac caaatcggcg
     4861 gcgtggtttg gcctacccac tggccattgg tccagttgat ggtgatgtgg acatccccgg
     4921 gcaggttctc attgagcagt ttgtaatcaa aactgacccg aatccacttg ccgcctttga
     4981 tgaacgggtc attccaccac tattcgttcc accacggaaa aaccggctgc gggtcttctt
     5041 gcggagccac atccggctgc gggtagcggt accactggcc ctcaaagccg gagttcaaag
     5101 ccggatccca aaccagttca tcggggccaa tcagatcaac ctgaaaatac ccctcacgaa
     5161 cctctgcggc tccaaccgct gtcagcagca ctgcgatagc tattccaaac acatctgtct
     5221 ttttcattat caaaccctcc aaaaagtacc gttcgttgct gttccgacta ttttgaaaag
     5281 gcctgctgcg gcgccgatgt tcatcccggc tgtccgaagg ccttgctgac agaacggcag
     5341 cgcgtatgtt ctgcgatgct gactgtgctg caaaatcgag aaatgggcgt aaaaaagaaa
     5401 atgaataaca actcgctgcg ggaaatgggt gatctttctt ttctcgtcct ttaatgtttt
     5461 gtcaaacaaa caattaaatg tccaagagaa aactgccaag cggtacatag cgtatcttca
     5521 aaccgcgcaa aagcaagcaa aatatcgtta tttttgcaaa aatcggtatt tttccggacg
     5581 ctgcggtttt gcagaccatc cagtcttttt agccgaaccg gcatttttac cgctgccatg
     5641 cggtttgttt gcatcacaga ccgaaaccgc attccggctg ctggggatcg gtgcaggcaa
     5701 gccagtcttg ggccattagc accaaatcca gcaggtttac gaagcagttt ttatcaaggt
     5761 cgccggcttt atagccccaa tcgccgcatt gcagttcaag tgtgcccttg aacaaatcga
     5821 tgagttcggt ttggcggttc cagaccgacc agaccacctg ccggccggaa atatccgctg
     5881 ccaacagttc atccgtttcg ctttccagca gagtccagac ggtaaagtgc ccgttgtcca
     5941 tcaggtaagc cgccttgagc cgatactggt atagtccggc cgtgccattc atctgggcta
     6001 aagcgggcat ccgggtagtg gtaatccaca cagcaatcgg tccgtcaatc ctcgggccgc
     6061 tgaccagctg ctcgccgtct tgaccctcct gccagagaac ctgaatctgc caattgtccg
     6121 tgtcctgata gttgtgaacg cccaaaatcc gatagggcca tccttcggag gcaaccagcc
     6181 acgagccgga gacctcaaag tgcgtccagt atcggtcgta atcgccgggc agtactgccg
     6241 tgcggatgac gggctcctgc ggattggaaa catcggctat gcggaccgac cgcgtgagtt
     6301 cgttgtaatt ggtgatatac cataagatgc tgccggcctc ggcagtcagc ccatagacgg
     6361 ttacctcatc cggttccaca taaacggtat aggtttgcgg attgttcagt tcagacgcat
     6421 ccacagcata gatggtatcc ctccagctgt tgagctggtc atcgccggca taagcaatca
     6481 gcccgccggc atagtcgaag tacagtaggg aaggaatctg cagcccgata tccgccaaga
     6541 ccggactgcc cggatcgctg atgtcggcca cccgcaggta ctgcttgtcg agcggccaaa
     6601 ccggatacac cgcccgatgc ccgcaaaagg ccagtgtctg gatatcggca gcgttggtgt
     6661 caattgcaaa ttcctgaaag ttcggatcgt ccagccggac gccgtacact ctgggaatcc
     6721 acatcgggtc gcggtagtcc agccacaccg ccgtgctgcc gcaaacggca aacgactgct
     6781 cccttccgta aacctgatgg tctggggctg cggtaatttc tgcgatgctg ccttcggcaa
     6841 aatacattgc cttgcatacg gaactgctca aagccgtcag cacggccagc aggcagataa
     6901 gttgtgttgt tttcattgtc atcgcctcct gtttttgtgg tgatggataa gagttgcgtt
     6961 ttggctttct gctgactgcc agcccaacac agacaggcca acattcaaca ggcggcaggt
     7021 tgttgcagtc ctgcccggct ggttgacaga gatgaagaaa accgcacgta ttacggtgca
     7081 gtataccgcc tcagaaggca aaagcaagaa aaatccctgt ttttggctta aaatcggtat
     7141 ttttccggac taagatgttt tttacctgct gggcgggatt ttttgacctg aatgtccgca
     7201 gccgcgcatt tcggataggt tttggcaggt atgcaagcca atagatgcct tcatagcgga
     7261 tgccaactgt ccagatagtc ccaattctgc atgcactatt ttgctatctg caaaaaattg
     7321 aaaaaatttt atcttcttgt atttgcgaaa gttagaaaaa atcgcctcga ttttgagccg
     7381 caattttcgg acaaaatctg caggatgccg ggtaatacgg agacagttga aaaattgaaa
     7441 attaaaaaac agaaggaagc agacaaatgg gatttgacct gaagattttc aatgatgcct
     7501 cgctggagcc gtcgtatgtg gaatggcttg ttgagcagcg atggccgcag gcagcccaat
     7561 acttcgggcg gctgtgggat tattacacca acccgctgac cgaaacagac cgcagcagcg
     7621 aaagcagccg ggggtatatg caggcgcagg aaacggggct tccgctgcgc atcacgggcc
     7681 agccggcacg ggaaggcggt cagattcagc gaaaagaagt cgtcattgaa aacgatatcg
     7741 gctggcgcat caatgcgatg gtagattttc tgttcggccg gccggtttcc atcaagagca
     7801 aagccgctgc agcgcatcgc cgcagagaca tagagggagt tcttaaagct gtgttcgatg
     7861 gcagcggcgg aacggttttc tttcaggaat tgggcacgct gggcagcgtc tatggttttg
     7921 tggactgcat cgtccgctgg ggcggccagc tggccggacg tcttgccgac agcacctcca
     7981 atcgcctcct ttgcgtccct ccttcatcct acgacagcgt gttgcgtctg gccggcgatg
     8041 tgtcgctgga actgattgat gcggcccgag cgctgcccgt cctggaagaa acggattaca
     8101 agcggattcg ctattatgtg cagcattttg tccagtggaa aaatgcggcc gccggggagt
     8161 cgtcatttct gggccggctt ctgggcacag gcgccctcgg ccagcgccgc tgtgcggccg
     8221 ttaccgagat tctcgggccg tcggcgtggc agcggtatga agacggaatc ctgacggcgc
     8281 agggtgtcca tccgctgggg cggattccgg tggtgcatat tcagaatctc ccccagccgg
     8341 gatgctacga gggcttaagc gatgttcagc agctttgccc cctgcaggat gagctgaaca
     8401 cccgtttaag cgaccgcgcc agccggatta ccatgcaggc tttcaagatg tatctggcca
     8461 aagggctgga cggaatcgcc gagcggccta tcgccccggg gcggatgtgg tgcacagaca
     8521 atatggatgc ggccatcgaa gagttcggcg gcgatggcgg aaatcccagc gagaaccagc
     8581 atattcagga agtgcgcgag gcaatggata aagtcagcgg cgtaacgcct gtggtggccg
     8641 gggtgctcaa aaacaagctg ggcaacctgt ccagtgcggt ggcattgaag atgaccttta
     8701 tgggaatgct cagcaagacc tgccgcaagc agctgaccta tggtcagggt atcagcgaca
     8761 tctgccggct ggtgctggaa gtgctggaca aagcggctgt gtttcccacc cgtccggccg
     8821 aacggcaggt cgaggtggta ttcagcagcc cgctggacga gcagataaac cgatagaaag
     8881 ccgtcggtct gccgccggca gattacgggc agtgcttctt tgacaagtga atagagtcca
     8941 ttttttttta actggacggc gcaaccggca gcagtcagaa cgtctggagc gtatcgggat
     9001 tcaatgccgc ctccagcggg acattgctgt tggcgatgtc gtccagaatc tgctgagccc
     9061 attcgtactg atagggcatc atatgcaggg gaagccattc gatccccagc tgcgacagaa
     9121 tctgccggca ggcgcagcgg gaattgtcgg tttggcagaa ttcagtccgc agcggctggc
     9181 tgacggcagg catataggtt cggctctgct gataaaacga gcactgattt gcataagcgc
     9241 ataatgtgtt ttctgcagca ttcatcgtta ttgttcccaa aggcagtttc ttttctgaag
     9301 cttgttttgg atgagtatat tgtcgtacat tcgggggcgg atgcattagc aaaaattgcc
     9361 gtttttccgg ctgataatgg gctttataaa gagttttgaa cgtattgcat attacgcaag
     9421 cagatatcca gaaacaatct ggaaatccct gtgcgtgaat tgcaaagttc gcagaaaaaa
     9481 acagataaca gcctcgaaaa acatcaaaaa acacgacctt tgatttcgga aatatctctg
     9541 cgatgaaggc cgaacaggga tgaaaaaaac tgaaaaaaat ttttgaattt gtgaaggaga
     9601 aggcaatgtt tgtctcaaaa aaacagaaat ccagcagccg gcacagccga cagcagcgct
     9661 tttgtgaggt cttgtaccgc actacggata taacactggc cgatgcaatg agccagtgcg
     9721 gcatatcgcc aaagactttt gccgattgga tggacgattc cagcctgcat gcagcactgc
     9781 agctgcagtt tcgcatcaaa ctgatggaag ggcgaagcat tatcggtcgt gccgtgccga
     9841 tggcagcggc caaactggtt gaactgctct gcaaagattc ggcacaggca gccggttctg
     9901 acaaaaaccg agatgaggcc atccgcaaaa cctgtttgga tattctgtcg ctgggcaatg
     9961 cctatgagca gtctatcgcc tgtcaggata agccggatac agccccgctg ctttcggatg
    10021 aagaagccga aaagctgatt cgggcgttga cggaggatga agatgatccc gacgatggcg
    10081 acggccaaga caatgggccg ctgtacaatc aggccaaagg ggatacagaa cctctgtgag
    10141 cagggctttt gttgtgaacc ggccggcggg gtcagcgtac aattgaaaac aagcagataa
    10201 accgtccggt ttattgcaga ccggcagcgg agccggcagt gaggcaaagc caaaacgatg
    10261 gaaacggcag cagaccatat cgaagcggtt cttgaacaag ccgctgtatc gtccgaactc
    10321 aaagagcggg cgctggaggc caccgccgaa ggggttgtga ttgccgactg cacgcaggaa
    10381 gggatgccga ttatctatgt caacagcggc tttacggcaa tgaccggata tgcggcacag
    10441 gatgttctgg ggcgcaactg ccggttcctg cagggagccg acagcgaccc gcaggccaaa
    10501 gaaaccattc gtcaggccat tgctcgccgg cagccctgtt cagtggagat tctcaactac
    10561 cgcaaagatg gaacgccatt ctggaatcgg ctgtcgatta cgccagtgcg cgatgccgcc
    10621 ggacgggtga cgcattttat cggcatccag acagatatta ccgtgcggcg caatgcggaa
    10681 gatttgctgc gggcggccaa agaacagctg gagcaggcca atgccctgct gcatcgggat
    10741 ttggaagccg ctgcggcgat tcagcagaca ctgctgccgg ccgagctgcc ggaagcggca
    10801 gggtaccgct tcgggtggcg gtatcgtccc tgccgacagc tgggcggcga tacgctcaac
    10861 atcacccggc tggacaagcg gcatatcggg ctgtatgtgc tggatgtcag cggccacggc
    10921 accgccgctg ccctgcaatc cttttcgctc agtcagacct tggggcggcg cagggagtat
    10981 tcgtttttgt atcggcagtc cggccggcgg caccgggttt gtccgccgca ggaggtgctg
    11041 cggcgtctga accggtcgtt tccgatggat atgcagacgg gcatctattt tacgatgctt
    11101 tacgggctgc tggatacgca gaccggcgtg tttgcgtttg ccagtgcggg gcatccgggg
    11161 ccggtgctgg tgcgtgccgg aagccggccg gaaatactcg atgcagacgg ctttccaatc
    11221 ggcgttgtcg aggaggccga ttatcgcctg cagaaagtgc agctttgcag cggcgacaag
    11281 ctgatactgt ataccgacgg tgttgtcgag gcgctcagcg cacgcgaagc cgcattcggg
    11341 cgggaacggt tcttaagggc gcttgttaaa accagtggcc agccaattga ggcgtgtctg
    11401 gatgcggtga tgacggcgct ggagcattgg gcctgccata acaatctgcg cgacgatgtc
    11461 tcgctggcag ggctggaaag gatttgacaa agtccgccgc agagcgaatg cagcggagcc
    11521 caattgccct ctgcgaggcg gctggaaaag gcggacggca ggttacagtt tcagtccggc
    11581 ggttaaagaa gccggcaggg cagaaagctg tttttcaagg gctttataaa ccttgacggc
    11641 tttggggtcg gttttgccgg cacgggcata attggcaagc accacaaact cgccgggatg
    11701 gcgtgtcagc gacagaaaga caggctgttc gggatgcttc cataaccgtg cagagtcggc
    11761 cggggcagcg ggattgagct gttcgctgtc ggcgatgctg agcaggtctt cttcctccgc
    11821 ccgggcggtg tagggcacaa ggccggccgc ggcggcgatt ttctcgacag cggcctgcac
    11881 atccagcggc tgaatcggca gggtttgcct gtcggctgct gacagcggga tagacatctc
    11941 ggcagaatac gacggctgac agccggccaa cagccagcag gctgccgctg cgaccagaac
    12001 aacaagatat tttgcactca taggcattcc attataaatt gttggttgcg gtctgtatgg
    12061 taagcccgcc ttgcaggatt gtcaaatctt ttcccagcca gagccgctgc ggcagcgtca
    12121 ggatttagaa gcccactgca tcgcacggca caggagtgcg gcggcaaaca gcaccgagct
    12181 gccgacaaac acactgccgg catctttgct gaaggcatcg acggcgctgc cgatgaccag
    12241 aagaccggaa acaaacaacg ccgccatgac gattaaatcg gcttgacctt tcttttccat
    12301 tggaatgtct ccttaaatgt gttcgccatc ctttgcatgc aaggataatt gtttgtcgga
    12361 aaatgtaaac agaaaaatgt ttttgcagaa aggagcagtt atgacagaca ccagtcagcc
    12421 cacggccgta aatcccgaac agcccgacac accggacagc ggacagctga accggctgcg
    12481 ggaggaaatt gagtcgctga aggcagatct ccgcgcgctg caggccgccg cagccggcgg
    12541 cgaggccccg ggcgcaggcg gcatccagcc caagacgcag ggggtgcggg aatcgctcgg
    12601 ggcaggccgg cgtgcggctt tggaacgggc agcgcgtgcg gccgcccgca gcggcagccg
    12661 gattgatgtg caggaatata tgcgcatccg acggagtttt gtgtagaact gtgccgcagg
    12721 ccaacagcac gatgccgtgg cacacacagc aggtttatag caattgataa ggaaggagtt
    12781 tgcatatgag tttcagcgga aaggcaacct acagtgccgg tgttaatctg ccggaattgg
    12841 ccgaagatgt atcggatgtg atttccattg tttcgccgta tgagacgccg ctgctggatg
    12901 ctctcggcga tccggttcgg gcggcgacca gcacgcatca cgaatggctg gaagatgcgc
    12961 tgctgccgaa caaggatgtg attgccgata cgaccatcgg cgatccggaa acggaaacgg
    13021 agtttgatgt agcccacccg gaccggtttc gccccgggga ccagattcag ccgcaggggt
    13081 cgggagaact gatgctggta acggcggtat cgggcagcac gctgacggtg atgcggagct
    13141 atgccggcac ggtggcggat gcgattgctc ccggccagac gctctgcatt ctgggcaatg
    13201 cggcgctgga aggcggcgac aagccggcgg cgcggtttac cacgcgtcag cggtgcggca
    13261 actataccca gattttcacc gccgcggtgg aagtcagcgg caccgacctt gcggcctcgc
    13321 agctggggct ggccgatgag atggattacc agaagcagga gcggctgcgc gaactgctgc
    13381 gggatttgga aaacaccgtc atcaacggcg gggccccgac ggccgacccg cagggcagca
    13441 cctcggtgcg gcggacgatg cgggggattt tggcgcatct ggtcagccat gtgtttatgc
    13501 cgggagtaaa cggatttccg tcgggtgcga cgctggatga agacaagctc aactgggccc
    13561 tgcggaaaat ctgggaagac agcagcggca cggtggatat gattgtcgtc gggggtctgc
    13621 agaagcggcg gataaacggc tttttaagtg cgctgcgggg ctatgcatcc ggcgatacgg
    13681 tctatcgcga cggcgtcagc gcctatgaaa gcgatttcgg gctgtgccgg attattacga
    13741 cgcggtggat gccggccgat gcggttctgc tgctggattc gtcgcgggtg aaggtgctgc
    13801 cgctggccgg ccgaagcttt catttcaagc ccttggccag cgggggcgat tatgaatgcg
    13861 gccagctgat tggcgaatat acgctggagc tgcgcaacga gtcggcccac ggggtgattc
    13921 gcggattggc agtcagttaa gctgtgcagg gggcggcagc gccgatggcg ctgctgcctt
    13981 gtttgcaggg atagggggat tggtttgtgc gggggattat tcctgcgccg gcgcttcgtg
    14041 gagttgggca gcgggtggtg catcagcggg gtataatcgg ttcaggacgg gtctcaggag
    14101 ccggttccat tgatcgtagc ccttttggtt gagatgaagg gcatccggct caaagagggt
    14161 ttcatcgggc aatccgtccc cgcccagcag cacggaggcg gtatcgacat agaggatgta
    14221 gccggcagtt tgggcgtatt gccggttgag ctgatttgcc tgctgcatct tcggccagag
    14281 tgtccagcgg ctgcggctgg gcttgaccgg caggcagata atcggcgtgc ggggcagttc
    14341 agcgtgaatg atttgcgtca gccggacaaa atcgcggtgg acttgctcgg ggggcaggcc
    14401 gccggctatg tcgttgtcgc cggcataaat gaccacaacg gccggcttgt agcgcaggat
    14461 gagccggtcg gcaaagcaga cgctgtcggc catccacgag ccgccgaagc cgcggttgat
    14521 gacgggcagg ccgctgaagg cctcggcggt tttccacagc cgaatgcttg aactgccgac
    14581 gaagacaacg gcattctgcg gcggggtgtt tttggcgtcc cagtcggcga aggttttcat
    14641 ctctccggcc cagcggttgg ggtcgggata gtcaacggtt ttctcggcgc cggccggcag
    14701 tgcacagccc agccggacag tcagcaggat acagaacaaa cagtgcatag cgttttttcc
    14761 tttcccattt ggtttcgtgc ggaagcattg tacgggatgg cttcggcaca ggcaaggttt
    14821 gaattgaccg aaaggaggta ttagaaatga gcggattttc cagcgatgcg gatttggcac
    14881 ggtacgagcc gggggtgttc gaccggtgga ttttgaacgg acaggtcctg tgcagaggga
    14941 ccaacggact tcttgcaggc acgcagtttg ccgccggcgg tgcggatttt acggccgcac
    15001 aggtcaaagc cggacatatc ctgtggctgc agagcacgga tggaagcatt gcgggggcgt
    15061 atgagattgt ccaagtgccg gacagcgggc atctgacagt atcggtgctg cgggctgatc
    15121 cccagcagcc ggcgataccg gcgggtgcgg cgtcggggct gctgtggcgg attgtgtcgt
    15181 atgatgtgca ggcctatgag gtccaatgcg
//
LOCUS       DAOTBO010000071        14722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3439_length_14722_cov_5.533647, whole genome shotgun sequence.
ACCESSION   DAOTBO010000071 DAOTBO010000000
VERSION     DAOTBO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 14722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14722
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_14722_cov_5.533647"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2979
                     /locus_tag="PLE88_08860"
     CDS             <1..2979
                     /locus_tag="PLE88_08860"
                     /inference="COORDINATES: protein motif:HMM:NF036710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOM76611.1"
                     /translation="VYLGTSQTAVAEARRPAGDIDGDGIADISDLAALAWQWLAAAAA
                     PCPDLDYSGRVDLLDAAVLSRHWNLSADVVYLGAVDGSSMEAGSLNPNKTYYWRVDTV
                     SCDGIERGAIWNFKTKRPAFPGAEGFGKWASGGRGGTVYHVTNLNDSGAGSFRDAVSA
                     PNRTVVFDVGGVIRIGERIIVSKDITIAGQTAPGEGVAIYGNGLSFTDANRSITRYMR
                     FRMGVIGTSGKDAVTIADGTDMIFDHCSISWGRDENFSISGGTGEDPGLITIQNCIIA
                     QGLETHSCGGLIQDFNGVSIFRNLYIDNDTRNPKVKGINDYTNNVVYNWDDAAYILGD
                     SEANSYANVRNNYFISGPNTGAAAFTRGNLNFHIYADNNWHDANRNGVLDGRLLLQAD
                     YGTVDWQAAPYDYPSVKTMLDPLTALKVVASQAGASVPMRDKIDTRLAKELLSYGTSG
                     QLISNENNSPMYGIGLLDSGHAPADTDQDGMPDYWEACIAGLNPNAADHNGDADGNGY
                     TNLEDYLNWLAGPHSDVQKNAYADIDLRAFTSGFNAGAAYTVFGATHGTAALLADGYT
                     VRYVPDADYVGRAEFRFTVDDGSVFADTVQLLVSPFGGHPLPAVYPAAAVNGLDYAYY
                     EGTWDYLPDFAALTPVQQGTAANFDISQAPSADGFAYVFEGFIEVPADGQYTFYLYSD
                     DGSKLYIDNGIVVSNDGVHGSQERSGKIALRAGKHSIRVRYFEKTGSQRLEVRWAGPG
                     FGRQLIGNGVLYRGSLDTAAPAKPTGLWAAAGSTAVTLDWNDSTDSDLAGYHLYRATV
                     SGGPYTRLHTGLLSTSNYTDSSVVGGTLYHYAVSAVDTAMNESAKTSEVSALLGAVPS
                     VIIQENTVGFCGVEGTVDNNNAGFTGDGFANGTNAVGAGIHWKISTAAGSYTFKWRYA
                     NGVTADRPAKLLINGIEAAASVSFPSTGAWTTWSEVSQTAVLTAGLQTVRLEPTTSSG
                     LANIDYLMVSGIDAVPVNCN"
     gene            complement(3161..4753)
                     /locus_tag="PLE88_08865"
     CDS             complement(3161..4753)
                     /locus_tag="PLE88_08865"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOM76612.1"
                     /translation="MAAGPQSNSIAAPYVSKVWIADNGDGTYKNPILYADYSDPDVIR
                     VGDDYYMTASSFTCFPGLPILHSKDLVNWTLIGHAVPQYPDALFDLPQHGKGIWAPAI
                     RFHDEHFYIFWGDPDAGIYMVKSKNPAGPWDKPVLALAGKGLIDTCPLWDEDGNAYIV
                     HGWAGSRTGGFNSVLTVRRMKPDGTEISPEGRHVFDGHDRHPTLEGPKFYKRNGYYYI
                     FAPAGGVTTGWQTILRSKSIWGPYEDKIVLDQGTTSVNGPHQGGWIETPSGQSWFIHF
                     QDAGAYGRIVHLQPVKWVNDWPVMGIDKDGDGKGEPVLTFAKPDIKGHFPIVSPAESD
                     EFDTDTLGLQWQWQGNPQITWYALLPGKDYLRLFALPLPHPEATLWDTPQLLLQKFPA
                     PDFSASVKITLTPVYDGKRAGLIIMGDDYACLSLNAKQQKLWLTQSVCLNAVKGGKEK
                     ILAEYPMDASAVYLQVQVSSPDAQCRFRYSLDGQQYREIGDVFTAKAGRWVGAKVGLF
                     CLSDPSARAGGYADFDWFRIDK"
     gene            complement(4864..6882)
                     /locus_tag="PLE88_08870"
     CDS             complement(4864..6882)
                     /locus_tag="PLE88_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOM76613.1"
                     /translation="MKKKCIISGQTVRHYFFNTVIVGTGAAGLNCAKRLYELMDQKGF
                     PEPHQRIALVTAGIGLGASRMSGSDKQTYYKTGTSPYVPDCAERFAQALTAGGCCHGD
                     LALAEAVGSLGSFYALAEAGVPFPTNAMGSFIGYKTDNDPSERAASAGPETSRFMCEC
                     LERIVRRYGIAIYENQEAADLLTVGTGTDKRIVGLAAVSKTNTAPAEWELSVFYCENL
                     VLAAGGPGGLYQTSVYPPGQIGLHGIALRAGLAAENLTESQFGLASIRFRWNVSGTYM
                     QAIPRFFSTDANGGGEKDFLADFFPTKEAMASAIFLKGYQWPFDAQRVEAHQSSLIDL
                     LVLGQMHNGRRVFLDFRRNPMDPFRPDKLESEALAYLTAAGALQQLPIERLEKMNPPA
                     VEIFRRHGIDLASVPLEVAVCAQHNNGGFAVNHWWESNIPHTFVIGEMAGTHGIRRPG
                     GAALNAGQVGAARAAEYIVNCYGSGLPDPDAVSAQSRRQIQHLIEKYSRWKTGRLATG
                     ETIEQIQQRMSTYAGPLRKLSEVRRAFCQALDLYQTIRTAGFRIQTAADLIAAIRAEH
                     LSLASAAYLKAIEELLSCGGGSRGSYLVLSDEGIEIDPAVQDPYTLKPLRFLPENKAL
                     RSTILRIAADENSDHLFACEHIPPRPMPKEIHSFEAAWADYRNGRIYK"
     gene            complement(6891..7667)
                     /locus_tag="PLE88_08875"
     CDS             complement(6891..7667)
                     /locus_tag="PLE88_08875"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOM76614.1"
                     /translation="MDTKPVAVVTGASRGIGRAIAVQLARMGYFAVINFCTDEKSAAA
                     ARQEIVSQGGSCECVQADIGQADGRQHLLEHIQNSRGRLDLLVNNAGIAPLRRQDLLD
                     VSEESYDRVMSINLKGPFFLTQQAALWMMEQKKMHPQRIFRIINISSISAYTSSTTRG
                     EYCISKAGLSMMTRLFADRLAEFDIGVYEIRPGIIQTDMTAAVKEKYDRLIAEGLTPQ
                     KRWGRPEDVAQAVAAVAEGRLDFSTGQVINVDGGFHLRRL"
     gene            complement(7670..9157)
                     /locus_tag="PLE88_08880"
     CDS             complement(7670..9157)
                     /locus_tag="PLE88_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOM76615.1"
                     /translation="MHTAANQPAQADTARGLPMFSGTPSEALEKEKALLAYVCHKSLW
                     ERTKVYLHLTGPGWMQSAMTLGASSASASLFAGALTGCRLLWVQPLAMLAGVVMLSAL
                     VHQTLSTGCRPFAAIRDYLHPGFAWIWALTSLLVTIIWHIPQYALAAGMTEDMIKAAT
                     GWQPNPRQQTLTLLLIGLCMMVFSVYITWSYGHRKRGVRIYEKVLKGFLWLIIAAFAL
                     VVINSFAEGKLSMAELLKGCIPNLPRYDDPVMQQKTTSLVIGAFSGAVGLNMTFLFPY
                     TLLARRWGREHRELGFFDLITGMLIPYSIATSLMMAAAGSTLYNTPAIEALIAENRPL
                     TPVLAASMLEQAGLNHYIARFVFGMGILGMVMSTISMQMLTAGFAVCEMFAIEPGGRL
                     YRLACLIPTPAFLGVLFWQKMSYWVAIPTAAICGIMLPIAYIAFFLLNNSRAYLKDDK
                     PVGTKAFVWNAGMLTAIGLSLAAGIYYVADVVWPYMMKLIGSLKG"
     gene            9277..10023
                     /locus_tag="PLE88_08885"
     CDS             9277..10023
                     /locus_tag="PLE88_08885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM76616.1"
                     /translation="MSEQQNNPFASVALGIVCPMANERDTVERFIPAVLGQCRGFRTC
                     TFFAVLDRASTDGTVEIVRSLAKQIEQLTMIWAPENRCVVDAYLRGYREALAAGCDWI
                     LEIDAGFSHRPQDIPRFFEAMKSGCDCVFGSRFCPGGVFEEAGFKRRLISRGGTVLTN
                     LLLGTKLKDMTSGFELFSRSALEMVLAKGIHSRAHFFQTEIKVFCRHLRCVEVPITYS
                     NPSSSVNGAVLGDAFFHLGRLFMLRLKGQL"
     gene            10035..11051
                     /locus_tag="PLE88_08890"
     CDS             10035..11051
                     /locus_tag="PLE88_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HOM76617.1"
                     /translation="MSPKTALVVTTIASPNEALAHLADGCGRHGMDFILIGDTKSPPD
                     FALHGCRYYSIQKQLETELIFAQKCPTRHYARKNIGYLLAIAEGCDVIFETDDDNEPL
                     EPFWTMPAFHLSVRVIENAGWVNVYRYFTDQTIWPRGLALDAVQQPLPAYERLEEKSV
                     YCPIQQGLVNSDPDVDAICRLVLPSAEYTFRTDRSVAVGAGSWCPFNSQSTRWAKEAF
                     MLMYLPACCSIRMTDIWRSFVAQRIAWENRWHILFHPPVMYQIRNVHQLMKDFEDEVP
                     GYLHNRRLCEAFEKLDLKSGLAHIPANLNRCYEKLVELKLVGPEELELLNAWLADFVR
                     VLRF"
     gene            complement(11048..12124)
                     /locus_tag="PLE88_08895"
     CDS             complement(11048..12124)
                     /locus_tag="PLE88_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76618.1"
                     /translation="MNKLTHEQQQLILDFYFRCGEPEDIERGRDLIASNHEAAVLYAS
                     LEQSLTELDHIKYEPCPDNLVDLTIARLKLAASCANASNSRLNDLLEQQALYKPERAS
                     QQTQKWNRNFLRPAFEILAAAASIVLAAGLLFPSFGAMRHYAQKNACAYNLRQIGAGF
                     ASLASISQDELAQARVPAGSPWWKIGDQGPQTQSNTRYPYQLVKQGLVSSSAFVCKGS
                     AGARPLENDPAILSRLYDFPSRSHVSYSFILWCDKTSNPFVGGRRIIAGDLNPVFRKI
                     PFQQDIYRQRDEFEQVLLNEELKKMLSENHCCRGQNILYSDGSIEYLKSRLINGDDIF
                     TVLGVDAYRGREVPASDRDAFLAP"
     gene            complement(12121..12747)
                     /locus_tag="PLE88_08900"
     CDS             complement(12121..12747)
                     /locus_tag="PLE88_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76619.1"
                     /translation="MNENETKSLSDKISDAQLLDRYRQGDEWAFKEIVNRYKNSLYAF
                     LRRFVSQEDIVEDVFQETFLQLYTSRDSFDEERPLRPWLFTIAANKAKDALRKMQRQS
                     SMSMGAIADAGDFSIDEVVNILTSYETTPDEEVSKEETAQRVRQIIAQMPENLRGILI
                     LAYFEQFSYKHMAEILSIPIGTVKSRLHTAVVYFMKKWKAANRGSDKL"
     gene            12981..13053
                     /locus_tag="PLE88_08905"
     tRNA            12981..13053
                     /locus_tag="PLE88_08905"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13014..13016,aa:Phe,seq:gaa)
     gene            13497..14165
                     /locus_tag="PLE88_08910"
     CDS             13497..14165
                     /locus_tag="PLE88_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76620.1"
                     /translation="MKRTFLLSITLCFCTILYFTRSASATVLAIWDFGKDSASYTESP
                     AYYNTVAAPILVLYGSGVDSNGKDGIPYTDAGGIAHIAGQAAAWDDINKSGTENDAAL
                     IITLNTTGFTNLAIRWDYKSELAVSYDFAYRTVAGGAWTQIVNNQPITPGWAEGKWYS
                     VVHDLSGYAALNNQPYLQLRIDDLVEGPGNDKFAIDNIEITGVPEPCTVLLLGLGGLI
                     IRKR"
     gene            complement(14504..14656)
                     /locus_tag="PLE88_08915"
     CDS             complement(14504..14656)
                     /locus_tag="PLE88_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM76621.1"
                     /translation="MKKERRRFEEAEKVKIIREHILDKRPLSEVCEQYGIAPTLNCRH
                     TDFQRV"
BASE COUNT         3429 a         3709 c         3960 g         3624 t
ORIGIN      
        1 gtatatctgg gaaccagtca aacggccgtt gctgaagccc gccggcctgc cggcgatatt
       61 gatggggatg ggatcgcaga tatttcggat ttggcagcgc tggcttggca gtggcttgct
      121 gcagcggcag ccccttgccc ggaccttgac tacagcggac gcgtggactt gttggatgcg
      181 gctgtgttaa gccgtcattg gaatctgtcg gctgatgttg tttatctggg tgcggttgac
      241 ggttcttcaa tggaagctgg gtcgctcaat ccgaataaaa cctattactg gcgtgtggat
      301 acggttagct gtgatggaat agaacggggc gccatctgga acttcaaaac caagcgtcct
      361 gcttttccgg gagcggaagg attcggcaag tgggcatccg gcggaagggg aggaacggtc
      421 tatcatgtaa ccaatctgaa tgattccggg gctggttcgt ttcgagatgc tgtcagcgcc
      481 cccaaccgaa cggttgtgtt tgatgtgggc ggggtcatcc gaatcggcga gcggattatt
      541 gtcagcaaag atatcaccat agccggacag acagcaccgg gggaaggcgt tgcgatttac
      601 ggcaacgggc tttcttttac cgatgccaac cgctcgatta cgcgctatat gcggtttcgg
      661 atgggcgtca ttgggaccag cggcaaagat gcggtaacga ttgccgacgg gacggatatg
      721 atttttgacc actgctcgat ttcatgggga cgggatgaga atttttccat cagcggcggc
      781 acaggcgagg atccgggtct gattaccatt cagaactgca ttattgcaca agggctggaa
      841 acgcattcct gcggggggct gattcaggac ttcaacggcg tcagtatttt ccgaaatctt
      901 tatattgaca acgatacccg caacccgaaa gtaaagggca ttaacgatta taccaacaat
      961 gtcgtttaca actgggacga tgcggcctat atcctcgggg attcggaggc caattcgtat
     1021 gccaatgtcc ggaataatta ttttatcagc ggacccaata ccggtgcggc ggcatttacg
     1081 cggggcaatc tgaactttca tatctatgcg gacaacaact ggcatgatgc caaccgcaac
     1141 ggcgttctgg acggccggct tcttttgcag gcggattacg gcaccgtgga ttggcaggcg
     1201 gccccctacg attatccgtc ggtcaaaacg atgctggatc ccctgacggc gctgaaggtt
     1261 gttgcttcac aggcgggggc gtctgtgccg atgcgggata aaattgatac gcggctggcc
     1321 aaggagctgc tgtcatatgg aaccagcgga cagcttattt ccaatgagaa taactcgccg
     1381 atgtacggca ttggtctgct ggacagcggt catgctccgg ccgatacgga tcaggacggg
     1441 atgccggatt actgggaagc gtgtattgcc ggtctgaatc caaatgcggc ggaccacaac
     1501 ggcgatgcag acggcaacgg ctacaccaat ctggaggatt atctcaactg gctggcaggt
     1561 ccgcattcgg atgtgcagaa gaatgcttat gcagatattg atttgcgggc gtttacatcc
     1621 ggttttaatg ccggcgctgc ttatacggtt ttcggagcga cacacggaac ggctgctctt
     1681 ctggcagatg gttatacggt tcgatatgtg ccggatgccg attatgtcgg acgggcggaa
     1741 tttcgcttta ccgttgacga cggcagtgtc tttgccgata ccgtgcagct tctggtttcg
     1801 cctttcgggg gtcatccctt gccggcggtt tatccggcgg cagcggttaa cggattggat
     1861 tatgcctatt acgagggcac atgggattac ctgccggatt ttgctgcgct gacgcctgtg
     1921 cagcagggca cggcagccaa tttcgacatc agccaagcgc catcggctga cggctttgct
     1981 tatgtgtttg agggatttat cgaagtgccg gcagatggcc aatatacgtt ttatctgtat
     2041 tccgacgatg ggagcaagct gtatattgat aacggcattg tggtcagcaa cgacggcgtg
     2101 catggctcgc aggagcgcag cggcaaaatc gctttgaggg caggcaagca cagcattcgg
     2161 gtcaggtact ttgaaaagac cggcagccag cgattggaag ttcgctgggc tgggccggga
     2221 ttcggacgtc agcttatcgg caatggggtt ctctatcgcg gcagcttgga tacggccgcc
     2281 ccggctaaac cgacaggtct gtgggcagcc gccggcagca cggctgtaac gctggattgg
     2341 aatgacagca ccgacagcga tctggccggc tatcatctat atcgggcgac tgtgtcgggc
     2401 gggccttata cacggcttca tactgggctt ttgagcactt ccaattatac agacagcagc
     2461 gtcgtgggcg gaacgcttta ccattatgct gtcagtgctg tggataccgc gatgaatgaa
     2521 tctgccaaga ccagtgaagt ctctgccttg ctcggggctg tgccgagtgt gattatacag
     2581 gaaaatacag tcggcttctg cggcgtagaa ggaacagttg acaataacaa tgccggcttt
     2641 acgggagacg gcttcgccaa cggtaccaat gccgtgggag cgggcatcca ttggaaaatc
     2701 agtactgccg ccggcagtta tacctttaaa tggcggtacg ccaacggtgt aactgctgac
     2761 agacccgcca aattgctgat caatggaatc gaggcggccg caagcgtcag tttcccgtca
     2821 accggtgcct ggacgacatg gagtgaggtc tctcaaacgg ctgtcttgac ggctgggctg
     2881 cagacggtac ggctggagcc gacgaccagc agcgggcttg ccaatattga ctatctgatg
     2941 gtcagcggaa tcgatgcagt gccggttaac tgcaactaaa aagacagctc ttagagcagc
     3001 ctatggctgt tggccgttct tgccgtatcg gtgtatgcag agggggcatt gcagaaaaac
     3061 gcccgcagcg atggggcggc tgaccgttcg gaacctgccc gcattgcatc cgaccggctg
     3121 aaatgcaggc gaaaacaggc agccggagtg tcggcagcgt ttatttatcg atgcggaacc
     3181 aatcgaagtc ggcatagccg ccggcccttg ccgatggatc ggacaggcag aaaagaccaa
     3241 ccttagcacc aacccagcgg cctgctttgg ctgtaaatac atcccctatc tccctgtact
     3301 gctgtccgtc cagcgaatag cgaaatcggc actgggcatc aggggatgaa acttggacct
     3361 gaaggtaaac tgccgaagca tccatcggat actcggcaag gattttttct tttccgcctt
     3421 tgacggcatt caggcagaca gactgcgtca gccaaagctt ctgctgtttg gcattcagcg
     3481 aaagacaggc gtagtcatcg cccatgatga ttaatccggc ccgcttgccg tcataaaccg
     3541 gagtcagtgt gattttaact gatgcagaaa aatcaggcgc aggaaatttc tgaagaagca
     3601 gctgcggcgt atcccacaag gtagcttcgg ggtgcggcag gggcagcgca aacagccgca
     3661 gataatcctt gccgggcagc agggcatacc aagtgatttg aggattgccc tgccactgcc
     3721 attgcaggcc gagtgtatcc gtatcaaatt catcgctttc agcaggcgag acaatcggaa
     3781 agtgtccttt tatatccggt tttgcaaatg tcaaaaccgg ttcgcctttg ccgtcgccgt
     3841 ctttgtcgat gcccatcacc ggccagtcat tgacccattt gaccggctgg agatgcacaa
     3901 tccggccgta agccccggca tcctgaaaat ggatgaacca cgattggccg gaaggtgttt
     3961 caatccagcc gccctgatgg ggaccgttga cggaggttgt tccctggtcg agaacaattt
     4021 tgtcttcata gggtccccag atgctcttgg agcgaagaat ggtttgccag ccggtggtga
     4081 ctccgccggc cggagcaaat atatagtagt agccgttgcg cttgtagaac ttgggacctt
     4141 ccagagtggg gtggcggtca tgaccgtcaa aaacgtgcct gccttcgggg ctgatttcgg
     4201 tgccgtccgg cttcatccgg cggaccgtca gcacgctgtt gaagccgccg gtgcggctgc
     4261 cggcccatcc gtggacaatg taggcattgc cgtcctcatc ccacagcggg caggtatcga
     4321 taagcccttt gccggccagc gccagaacgg gtttatccca cggcccggcc ggatttttgg
     4381 attttaccat gtaaatgcct gcatcgggat ctccccagaa gatatagaaa tgctcatcgt
     4441 gaaagcgaat agcgggggcc caaatgcctt tgccatgctg cgggaggtca aaaagcgcat
     4501 cggggtattg cgggacggca tgcccgataa gtgtccagtt gactaaatcc ttcgaatgca
     4561 aaatgggcag ccccggaaag caggtaaagc ttgaagcggt catataataa tcatccccca
     4621 cacggataac atccggatcg gaatagtcgg cataaaggat aggattttta tatgtgccgt
     4681 ctccgttgtc tgcaatccat actttggaaa cataaggggc cgcaatgctg ttcgattgcg
     4741 gacctgctgc catcagtaaa gttccctcta tgcatacagc ggcggccaag caggctgtta
     4801 ttttcggaca cctcaaaaaa tgcttcctgt tcatcgtgca tccctatcca tttctaaaaa
     4861 gttttatttg tatatccggc catttcggta atctgcccac gctgcttcaa aggaatgaat
     4921 ttccttcggc atgggtcttg gaggaatatg ctcacaggca aacagatgat cgctgttttc
     4981 atccgctgca atccgcagga tggtgctgcg caaggccttg ttttcgggca gaaaccgcag
     5041 cggctttagt gtataggggt cttgaacagc cggatcaatt tcgatgcctt catcggacaa
     5101 gaccaaataa gaacctcggg aacctccccc gcaggacaga agttcttcaa tggccttcag
     5161 gtaagcagcg ctggcaagcg acaaatgctc ggctcgaatt gccgcaatca aatccgcagc
     5221 ggtttgaatg cggaagccgg ctgtacggat ggtttggtat aagtccagtg cttggcagaa
     5281 agcccggcga acctcggaca atttccgcag cggaccggca taagtgctca tccgctgctg
     5341 tatctgttcg atggtttcgc cggttgccag acggcctgtt ttccagcgtg aatatttttc
     5401 gattagatgt tgaatttgcc ggcggctttg ggctgaaaca gcgtccggat cgggaaggcc
     5461 gctcccgtaa caattcacaa tatattcggc cgcccgtgct gccccgactt gaccggcatt
     5521 gagagcagcc cccccgggac ggcggatgcc gtgtgtgcct gccatttcgc caataacaaa
     5581 ggtgtgcgga atattggatt cccaccaatg attgacggca aagccgccgt tgttatgctg
     5641 ggcacagacc gcaacctcca gcgggacaga tgccaaatcg atcccatgcc tgcggaaaat
     5701 ctcgacagca ggggggttca ttttttccag ccgctcgatc ggcagctgct gcagggcacc
     5761 ggctgccgtc aaataggcca gcgcttcgga ctccagcttg tccggtcgga aaggatccat
     5821 gggattgcgg cggaaatcca gaaacacgcg gcggccgtta tgcatttggc cgagaaccag
     5881 aaggtcaatc agcgaagatt gatgtgcttc gacgcgctgt gcatcaaaag gccactgata
     5941 gccctttaaa aagatggcac tggccatagc ttcctttgtc gggaagaaat cggccagaaa
     6001 gtctttttcg ccgcccccgt tggcatctgt gctgaaaaag cggggaatcg cctgcatata
     6061 agtccccgaa acattccagc ggaaccggat gctggccaga ccaaactgcg actcggtcag
     6121 attttcagcc gcaagaccag cccgcaaggc gatgccatgc aggccgattt gtccgggcgg
     6181 atagacgctg gtttgataca accctcccgg cccaccggca gccaaaacaa gattttcaca
     6241 gtagaaaacc gaaagctccc actccgcagg ggcggtgtta gttttggaga cagctgccaa
     6301 gccgacaatc cgcttatctg tccctgtccc gacggtcagc aaatcggctg cttcctgatt
     6361 ttcgtaaatg gctattccgt aacggcggac aattctttcc agacattcgc acataaaccg
     6421 gctggtttcc ggtccggcgg atgctgctcg ctcagaaggg tcattgtcgg ttttgtagcc
     6481 gataaaactg cccatagcat tggttggaaa agggacaccg gcttctgcca atgcataaaa
     6541 actgccgagt gaacctacgg cctcggccag tgccaaatcg ccgtggcagc agccgccagc
     6601 cgtcagcgct tgagcgaaac gctcggcgca gtcgggcaca taaggggaag ttccggtttt
     6661 gtaataggtc tgcttgtcag agccgctcat ccgtgaagcc cccagcccga tgccggcagt
     6721 aacaagggca atccgttgat gcggttctgg aaatcctttt tggtccatca gctcgtacag
     6781 ccgtttggcg cagttcaacc ctgcggcccc tgtacccaca atgacggtat tgaagaaata
     6841 gtgccgaaca gtctgcccag agataatgca tttttttttc atgggtatcc ttagagccgg
     6901 cgaaggtgaa atccgccatc cacattaata acctgaccgg tcgaaaaatc cagccggcct
     6961 tcggcaacgg cggctaccgc ctgtgcaacg tcttccggtc tgccccagcg cttctgcggg
     7021 gtgagtcctt cggcaatcag acggtcgtat ttttctttaa cggcggcggt catatctgtc
     7081 tgaatgatgc cgggccgtat ttcatagacg ccgatatcaa attctgccag ccggtcggca
     7141 aacagacgcg tcatcatgga caaacccgcc ttgctgatgc agtattcgcc gcgcgtggtg
     7201 ctgctggtat aagccgaaat cgagctgatg ttgataattc gaaaaatccg ctggggatgc
     7261 atctttttct gctccatcat ccacagtgct gcctgctgtg ttagaaagaa ggggcctttg
     7321 aggttgatgc tcatcacacg gtcataactt tcctcgctga catcgagcag gtcttggcgc
     7381 cgcaggggag cgatgccggc attgttgacc agcaaatcca gccggccgcg gctgttttga
     7441 atatgttcca gcagatgctg gcggccgtcg gcctgaccga tgtctgcctg cacgcattca
     7501 cagcttccgc cctgcgagac aatctcctgc cgggcagccg ctgcggattt ctcgtctgtg
     7561 caaaagttga taacggcaaa gtaccccatt cgggccagct gcacggcgat ggcgcgtccg
     7621 atgccgcggc ttgctccggt tacaacagcg acgggctttg tatccatggt tatcctttca
     7681 gactgccgat cagtttcatc atataaggcc aaacaacatc agcaacgtaa tagatccctg
     7741 cggctaacga cagcccaata gcagtcagca ttcctgcatt ccagacaaag gcctttgtgc
     7801 cgacgggctt atcgtctttc aaataagccc ggctgttgtt cagcaggaaa aaggcaatat
     7861 aggcaatcgg cagcataatt ccgcaaattg ccgccgtcgg aatggccacc cagtagctca
     7921 ttttctgcca gaaaagaacg cccagaaacg ccggtgttgg aatcaggcag gccagacggt
     7981 aaagccggcc tcccggttca atcgcgaaca tttcgcagac ggcaaagccc gctgtcagca
     8041 tctgcatgga aattgtgctc atgaccattc ccaaaatacc catcccgaag acaaagcgtg
     8101 caatgtagtg attcaatccg gcctgttcga gcatcgacgc tgcaagaacc ggcgttaagg
     8161 gtctgttttc ggcaataagg gcttcgatcg ccggcgtatt gtacagcgtt gatcctgctg
     8221 cggccatcat cagcgatgtt gcgatgctgt agggtatcag catgccggta atcaaatcaa
     8281 aaaagcccag ctcccgatgc tcccggcccc atcggcgtgc taaaagggta tacggaaaca
     8341 aaaatgtcat attcagaccg actgcaccgg agaatgcgcc gatgacaaga gaggttgttt
     8401 tctgctgcat aacgggatca tcataacggg gaagattggg aatgcatcct ttgagcaatt
     8461 cggccataga caatttcccc tctgcaaagc tgtttatcac aaccaatgcg aaagcggcaa
     8521 tgataagcca tagaaatccc tttaaaacct tttcataaat ccgcaccccg cgtttgcgat
     8581 gcccgtagga ccatgttata tatacagaaa aaaccatcat gcacagaccg atgagcagca
     8641 gggttagggt ttgctgccgg ggattgggct gccagccggt tgcggccttg atcatatctt
     8701 ctgtcattcc ggcggccaag gcatactggg ggatgtgcca tataatagtc accagcagag
     8761 aagtcagcgc ccatatccat gcaaagccgg gatgaaggta atcccgtatt gccgcaaagg
     8821 ggcggcatcc ggtggagagg gtttgatgaa ccagtgccga cagcatcacc acaccggcca
     8881 gcatagccag cggctgtacc cagagcagcc ggcagcctgt taatgcaccg gcaaatagag
     8941 aggccgaggc gctgctggct ccgagggtca tcgcactctg catccagccc ggtcccgtca
     9001 ggtggagata gaccttggtc cgctcccata ggcttttgtg acagacgtag gcaagcagtg
     9061 ctttttcctt ttcgagggct tccgacggag tgccggaaaa catcggcaat cctcttgctg
     9121 tgtcggcttg agccggttga tttgctgctg tatgcacaaa atactcccga tctatttttt
     9181 tccttgcccg cgcattgtaa cagccccatc cagcagggtc aagattggag tacggatgaa
     9241 attgactttc agccgcattc gggatatatt aaatatatgt ctgaacagca gaataatccg
     9301 tttgcctctg ttgcattagg tatcgtatgc cccatggcca acgaacggga tacggttgag
     9361 cggtttattc ctgctgtatt ggggcagtgc cgcggttttc ggacatgcac tttttttgct
     9421 gttctagacc gtgccagcac ggacggtacc gtggaaattg tccgatcgct ggcaaagcaa
     9481 atagagcagc tgacgatgat ttgggcgcct gaaaaccgct gtgtggttga tgcgtatctg
     9541 cgggggtatc gggaggcgct ggcagccggc tgcgactgga ttctggaaat cgatgccggc
     9601 ttcagtcatc ggccgcagga cattccccgt tttttcgagg ccatgaaaag cggctgcgat
     9661 tgtgttttcg gcagccgttt ctgtcccggc ggtgtttttg aagaagccgg attcaaaaga
     9721 cggcttatca gcaggggggg taccgttctg accaatctgc tgctgggtac gaaattaaaa
     9781 gatatgacca gcggattcga gcttttttcc cgttccgctc tggaaatggt gcttgccaaa
     9841 ggcattcact cccgggccca ttttttccag actgaaatca aagtcttctg ccgacatctc
     9901 cgctgtgttg aagtccccat cacctattcc aatccgagca gcagtgtcaa cggggctgtt
     9961 cttggtgatg cgttttttca tttgggacgg ctgtttatgc tgcgcttaaa aggtcaattg
    10021 tagccggagt ctttatgtcc cccaaaactg ccttggtagt aaccacaatc gcttctccta
    10081 atgaggcgct ggcgcatctg gctgacggat gcggccggca cggcatggat tttatcctta
    10141 tcggcgacac caaaagcccg ccggattttg ccttgcacgg atgccggtat tacagtatcc
    10201 agaagcagct ggagaccgaa ttgatctttg cgcagaaatg ccccacacgg cattatgccc
    10261 gcaaaaatat cgggtatctg ctggccatcg cggagggctg tgatgttatt tttgaaaccg
    10321 acgatgacaa cgagccgctg gagccgttct ggacaatgcc ggcatttcat ttatccgtcc
    10381 gtgtgataga aaatgccggc tgggtgaatg tgtaccgcta ttttacagac cagactatct
    10441 ggccgcgggg attggctttg gatgcggttc aacagccgct gccggcttat gaacggcttg
    10501 aggaaaaaag cgtctactgt cccatccagc agggtctggt caacagcgac ccggatgtcg
    10561 atgccatctg ccggctggtt ctgccgtctg cggagtatac atttcgaacc gatcgcagtg
    10621 tggctgtcgg tgccggaagc tggtgtccgt tcaacagtca atccacccgc tgggccaaag
    10681 aggcgtttat gctgatgtat ctgccggcct gctgcagcat tcggatgacc gatatctggc
    10741 ggagctttgt tgcccagcgg attgcttggg aaaaccgctg gcatattctc tttcacccgc
    10801 ctgtaatgta ccagattcga aatgtgcatc agttgatgaa agattttgaa gatgaggtgc
    10861 ccggctactt gcacaaccgg cggctatgcg aggcgtttga aaagcttgat ctcaaaagcg
    10921 gcttggccca tattccggcc aacctcaacc ggtgttatga aaaactcgtc gaactgaaac
    10981 tggtagggcc tgaagagttg gaactgctga atgcttggct ggcagatttt gttcgtgttt
    11041 tgcgattcta aggggcaaga aacgcatcgc ggtcgctggc gggaacttca cgccctcgat
    11101 aggcgtcaac gcccaaaacc gtgaagatgt catccccatt gatcaagcgg cttttgaggt
    11161 actcgatgct tccgtcgctg tatagaatat tctggccgcg gcagcagtga ttttcgctga
    11221 gcattttttt cagctcttca ttcagcagca cctgctcaaa ttcatcacgc tggcggtaga
    11281 tatcctgctg gaatggaatt tttcgaaata ccggattcaa atcgccggcg ataatccgcc
    11341 gaccgccgac aaaaggattg cttgttttat cgcaccaaag aataaagctg taactgacgt
    11401 ggctgcgtga ggggaaatcg tataagcggc ttaggatggc aggatcgttt tccaaaggcc
    11461 tcgctccggc ggaacctttg catacaaatg cgctgctgct gaccagtccc tgtttgacga
    11521 gctgataagg ataacgcgta ttggattgag tttggggacc ctgatcccca atcttccacc
    11581 aaggagaccc ggcgggcaca cgggcttggg ccagttcatc ctgactgatg ctggccagtg
    11641 aagcaaaacc tgctccgatt tgtcttaaat tataggcgca cgcgtttttc tgtgcataat
    11701 gccgcattgc gccgaacgaa ggaaacagca ggccggctgc cagcacgatg cttgcggccg
    11761 ctgccaatat ttcaaatgca ggacggagaa agtttctatt ccatttttgt gtctgctgag
    11821 aagctcgctc gggtttatag agagcctgct gttcaaggag gtcattaagc cgggaatttg
    11881 aagcattggc acagctggcg gccaatttca gacgcgcaat ggtcaaatct accagattgt
    11941 caggacaggg ttcgtatttg atatggtcaa gttcggtcag agattgttcc agactggcat
    12001 aaaggactgc ggcctcatga ttggatgcta tcagatcgcg tccgcgttcg atatcttcgg
    12061 gctcgccgca tcggaaataa aaatccaata taagctgctg ctgctcgtgg gttaatttgt
    12121 tcatagctta tcagatccgc ggtttgctgc tttccatttt ttcataaaat aaaccactgc
    12181 tgtgtgaaga cgacttttaa cagttcctat aggtatactt aaaatctcag ccatatgttt
    12241 gtaagaaaat tgctcaaaat aggccaaaat cagaataccc cgcaggtttt caggcatttg
    12301 cgctattatc tgacgtaccc gttgagctgt ctcttctttg gagacttctt catcaggggt
    12361 tgtttcatag gaagtaagga tgtttacgac ttcgtctatc gagaaatcgc cggcatcggc
    12421 tatggccccc atgctcattg aagactgccg ctgcattttg cgcaaggcat ctttggcttt
    12481 attggctgct atggtgaaaa gccaaggacg aagcgggcgt tcctcatcaa aactgtcccg
    12541 gctggtatag agctggagaa atgtttcctg aaacacatct tccacgatgt cttcctgact
    12601 gacgaaccgg cgtaaaaaag cgtaaagcga gtttttataa cggtttacaa tctctttaaa
    12661 cgcccattcg tcaccttgcc gatagcggtc cagcaattga gcatcggata ttttatccga
    12721 taaagatttt gtttcgtttt cattcattga ttgtgcagat gtaagaaaaa tcttttatgg
    12781 tattataaat tattgctgga taatttgaat cccaaatttc aaaaattata ctgtgtctgt
    12841 tttgatggga aagtcaatta gaccttttgt tttggcgacg gcaggaaatt tgattgccga
    12901 caggaagatt tctgaatttt ggtctttttc ccttgcaatc aaacattgga tttgctaatt
    12961 ttttttacag gttttagctc gggcaggtag ctcagctggt agagcaacgg actgaaaatc
    13021 cgtgtgtcgg cggttcaact ccgcccctgc ccattaataa ctccttatat ttcatatgtt
    13081 tacaaacatt tcttattata tctttttttc aaaaaagggt gtgcgccaaa gtgcgccaga
    13141 aagagagaga aagagagaag gaagagatgt catttatagc atgagaaagg aaaaaaagag
    13201 tttaggtttc gtgtgtttta gattctggag ttcgattgga tgagaaaata atgaacaggg
    13261 acagtcaacc tgtttctgtc cccatcactt atctgattgt catttaatcc gtaactgtcc
    13321 caatttattc tgtttcccat gcccgataat ttaccagatt ttcaggtatt tggcgacatt
    13381 tttgctttat tttcttgatt gtcctattgc cgattaggta taattggaac agcgggtgtg
    13441 aaatgttggg ggtaaactcc caacatttag aagccgcaat ttcggagttt agaaagatga
    13501 aaagaacatt tctgctttca atcacgttgt gtttctgcac tattttgtat ttcacacgct
    13561 cggcatcggc tactgttttg gccatctggg atttcggcaa agacagtgcc tcttatacag
    13621 aaagcccggc ctattataac acagttgctg caccgatatt ggtcttatat ggttcgggcg
    13681 tcgattccaa cgggaaagat ggcatccctt acacggacgc cggcggcatc gctcatattg
    13741 ccggccaagc cgctgcatgg gatgacatta acaaatccgg cacggagaac gatgccgcat
    13801 tgattatcac gctcaatacg acgggcttca ccaatctggc cattcgctgg gattacaaat
    13861 ccgaactagc ggtcagttat gattttgcct atcgtacagt cgccggtggc gcatggacac
    13921 aaattgttaa caatcagccc ataactcccg gctgggctga ggggaaatgg tactctgttg
    13981 tacacgactt gtcgggctat gctgcattga acaatcagcc gtatctccag ctccgaattg
    14041 atgatcttgt cgaaggcccc ggcaacgaca aatttgccat tgacaatatt gaaattacgg
    14101 gtgtccccga gccctgcaca gttttgctgt taggcttagg cggattgatt ataagaaagc
    14161 gataacatat tttaccacta ctgtatggat cggagaaggt ggaaaagttc tccctgtatt
    14221 catgtaatca gaggtggctg aaccccttcg gctcttgatt gatagcgagc cgacaggtaa
    14281 tctatatcgg aggagggcag ttcagaaatg gactgccctc ctgtgtttcc ctactctcct
    14341 tcccccatct tcctacctcc cacctcccct atgtgcacca ctttgtgcac caaccaagta
    14401 ctattgaaca actattggac agctattgga aagcactttt tttgatgtaa gttttttctg
    14461 gataagcata atgccttata tttgctatac ttaaaaaagc gccctaaacc ctctgaaaat
    14521 ccgtgtgtcg gcagttcagg gtcggggcaa tgccgtactg ctcgcacacc tctgacaaag
    14581 gacgcttgtc aagaatatgt tcccgaataa tttttacttt ttctgcctct tcaaatcgtc
    14641 tgcgttcctt tttcacaaca aatccctttc aaaaatgtcc acaaaagaat aacactttta
    14701 aaggatttgt ccatttccaa ct
//
LOCUS       DAOTBO010000072        14675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3449_length_14675_cov_4.808003, whole genome shotgun sequence.
ACCESSION   DAOTBO010000072 DAOTBO010000000
VERSION     DAOTBO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 14675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14675
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3449_length_14675_cov_4.808003"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLE88_08920"
     CDS             complement(<1..631)
                     /locus_tag="PLE88_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM76622.1"
                     /translation="MEKHEIKKHVTDMAIMIVIYLNTIGLADNPIIQTQYTADPAPLV
                     HHDRIYLYTTHDEDVLVNNFFTMNDWRCYSTTDMVNWTDHGSPLSYKDFSWASGDAWA
                     GQCVYRNGKFFFYVPMKMKVGGKAIGVAVATHPAGPFTDALGHPLVGNDGSWGDIDPT
                     VFIDEDGQAYLYWGNPYLRYVKLNEDMISYSGDVVEVPLTPAGFGLRSKD"
     gene            complement(706..3960)
                     /locus_tag="PLE88_08925"
     CDS             complement(706..3960)
                     /locus_tag="PLE88_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM76623.1"
                     /translation="MVSPCIGQNPIFRDVFTADPAALVVDDTVYVYVGRDNAKEGQFF
                     TMPEWRCYSSKDMKVWTSHGPIMRPEDFKYARPDTAWACHMIEKDGRYYFYATLRRNN
                     NNEHCIGVAVGDSPLGPFKDARGTPLITDDMTTDSSRPNADIDPAVFIDDDGTAWLMW
                     GNGDFYLVRLKPSMIELDGPIQKIPHPNVAEAPWLFKRGNLYYNIYAADVPGNPPEKV
                     AYATAEKITGPWTYRGLVTGSPKAGFTIHPAVIEFKGQWYFFYHDGSTSLNGIPGGDC
                     RRSVCAEYLYFNADGTIQPIMQTDEGLSLPPKVTQASAQLFTLRDVRLLESPFSQAVQ
                     ANRQYLLAHHADRLLAPFRREAGLSPKAQPYGNWESMGLEGHSAGHYLSAISLMIASG
                     ADPDGEFQRRLDYMIDELAQVQKANGNGYLGGISGSKEFWARIADGHVELIGKRWAPW
                     YNLHKMFAGLRDAYLVGGSKKSRDLLVGFADWCEKITSGLTDQQMQQMLGVEYGGMND
                     VLADIYVITGNKKYLELAKRFNHRSVFEPLENRQDKLTGIHANTQIPKIVGMERIAAL
                     THDEKLHTGAEFFWETVTQNRTVAFGGNSVSEHFNNPADFSGMIAHREGPESCNTYNM
                     LKLTEELFKAEPRAAYADYYERALYNHILASIHPQKPGYVYFTSIRPDHYRVYSQPEV
                     AFWCCVGTGMENPGRYGAFIYAQRMDQLYVNLFIPSKLSLNVGGVVLRQQTQFPDEPR
                     TQLHFQLLKPTVFTLNIRHPYWVKAGDFAVKVNGRLIDVKSTPCSYAAIRREWADGDT
                     VDVELPMHLHTERLPDGSDWVAFLYGPVVLAKPGSTEHMDGLFADGSRMGQVAHGPLI
                     PLDKVPTLLAAEEDLLKHIVEDRAAGPLHFRIKDIIEPDDPAGLPLMPFFRLHERRYQ
                     MYWELTTPQQIKTRREKLAAEERQRQVREAATIDWIAVGEQQPEVEHDLQGEGMETGI
                     HNGRRWRHGYWIQYTLDSRDAKEVILSVTYSGDDRGREFDILVNGTKIATQKLFGEKP
                     HDFIEKHYPVPAEILRTAENGCFTVRFTASRGLAGGLYDVRLLRLNASGSHPAN"
     gene            complement(4113..4649)
                     /locus_tag="PLE88_08930"
     CDS             complement(4113..4649)
                     /locus_tag="PLE88_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM76624.1"
                     /translation="MDPNSHSDGNHPGIIDYKGTTYLFGLNYELFFTLQGHRKKLERR
                     SVCVKTMTYNTDGTIQKVPWWGDGTAIPSVPQVGFLNPYQTVEAETICWASGVKTEHC
                     SEGGMNVCNIENGDYIKIKGVDFGRGAVFFRGSGLFRFRRRLYRDSSGQSRWDPGGNL
                     CSFQHRGMGNMEDRGVLG"
     gene            complement(4743..5429)
                     /locus_tag="PLE88_08935"
     CDS             complement(4743..5429)
                     /locus_tag="PLE88_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM76625.1"
                     /translation="MEKPIEISGIQKLILTMVMAVATAVGMLCYADNPIIQTKYTADP
                     APMVYDETVYLYTTHDEDDATGFKMFNWLLYSSKDMVNWTDHGIIAGVAEPYKTFKWA
                     DGHSAWAPHCIVRNGKFYFYCPTIFKGRMAIGVAVAESPTGPFVDPLDKPLIYRGNPG
                     DYDPAAFIDDDGQAYLYWGGNGPCYYVKLNEDMISTSGDIQTASIDFTGTPPEASYTE
                     GPWLWKKNNH"
     gene            complement(5514..8456)
                     /locus_tag="PLE88_08940"
     CDS             complement(5514..8456)
                     /locus_tag="PLE88_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOM76626.1"
                     /translation="MTKGSVVEKMVQIKFFSAFLFLCFSIVLQAAPSGLDKFGHLQSI
                     AGSDRKDFSFCTLENGIQLHVGDAVKNVIFYGSSTVRVNENLGRSYWEHPSIVVVGQP
                     AAVPFRIHETADSVAIVSDTLQIKADKQTGALRFMNASGKLLTCERAVNHAMIKQVEI
                     SGAPTYEVSNTFTLKPDEGWYGFGYIDSAPIQINRRGQELLLIQTNMGIVIPMIISSE
                     RYGIMWDIYSVMRFKDDAAGATLWAESAPGGVDYYFFAGDTMDDVIAAYRNLTGNAPL
                     YPKQALGLFMSKERYQTQQRIVEVARTFRKEQFPLDYIVQDWQYWGSDKDGTWSGMIW
                     NKDRYPDPEGMIKAIHDLNMKLMISIWPSVGNDTPLAHELDQYKLRFEPLHWISRKAR
                     IYDAFSEKGREIYFKHVKNGLLDKGVDALWMDGTEVEVGTACWNPNEVARDIKRLGSN
                     ALGDFSRYLNVYTLMTTKGTYEGQRAISNQRVFTLTRSAWCGAQRYAAASWSGDSSAS
                     WNMLKSQMASGLHVTMAGNPWWTQDIGGFFVNNFPGGEQNPAYRELFLRWYQFGAFNP
                     IFRIHGTNIEREPYIFRQMDAEVYDALLDITHLRYRLMPYLYSLSWKVTSEGYTLMRA
                     LPMDFPDDAGVRNISDAFMFGPAFLVHPVTRAMYRTESTPAPTVPTEFLRTPDGKPGL
                     AVQYFAGINFDTPKGNGIDEVVDHDWPGPPLAVLPGGLNDLNNFSARWEGFLVAPQDG
                     EYEIGVEGDDGFRLYLDGRKIIEDWRENAARYRSAKVTLSKGQQVPLKLEFYQAGGER
                     ILRLGWRTPSDIRNQREHETAVDNVVKTYLPAGADWYDFWTNEKLTGGKTAEKACPLN
                     ILPLYIRAGSIVPMGPVIQYATEKPDAPYEIRIYPGDDAKFTIYEDDNETYNYEKGEY
                     ATYDLVWNDAAKKLNIGARKGSFPGMVPTRQLKIVLAVSEENAGISDASRAVKSVVYT
                     GQDIALEF"
     gene            complement(9462..>14114)
                     /locus_tag="PLE88_08945"
     CDS             complement(9462..>14114)
                     /locus_tag="PLE88_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM76627.1"
                     /translation="FTSTEWSGAVPYEEIAKIKNLGFNAVHLYGECFDINYPNPGSTA
                     PGYAAARIDSIVQSTRNLGLYLILTIGNGANNGNYNRSYVEDFWRYYAPRYANETHVL
                     FEIQNEPVAWGPPYLTHTNPPGAVDMEISAYNIIRSYAPDTPVLLFSYAVPWGSGGAN
                     DALIDVRAFNTAVFGNPDAIWTNEAVAIHGYSGSSLTSDFAAAMIGFGYPCFQTEFAG
                     GIWGEEGGGFELYTASEMERRKVSWLTFQFIPPWGVSDCITIPKVYKDRVDRSGLSWM
                     PDYGIWPQIRSSYGSGGQPRSTTGLSGTLRIQAEDFDIGGPGVAYDDMTMSNEGGQYR
                     TAESVDIEVTSDSGGGYNIGWTMDGEWLEYTIFVTEAGYYNLRLRVASPNTGCAARVV
                     SYNLDKTGRWTIPNTGGYQNWTTITKQVFLEYGRQKLRVEIVTGGFNLNWIELSPAAV
                     GPVTDSVYKIVNQNSGLAIQNNNRNVIQSSFIDAATQKWSLQHQGAGQYRVRSAYDNW
                     QWNTCILQPLGDGYYRLIREDSGLDCGAENASLDAGAAIVFNEYAGLAHQKWAVLSPS
                     APAFPVGLTAEWSSSTQIDLFWTASAGATSYNIKRSVNSSGPYTIIAANITATSYTDT
                     DVINGRVYYYVVSANTLSGESLNSRPAVVTPWKSQDIGTAALTGSSIFNGDVFVVNGS
                     GSDIWGTADAFRFTYLTVNGDCTIIARVVSVQNTDSWAKAGLMIRESLNANSKNAFIA
                     VTPGNGVTWQYRTSTGGSSANSAATGLAAPYWIKLVRSGSTFSGYHSPDGITWTQQGA
                     SQTISMASTVYIGLAVTSHNNSSLCQAVFDNVTAPGWPLPLGPVAPANLSATMITSSQ
                     VNLTWTTALGATSYNIKRSTASGGPYTTIASTITLTSFSDTNITGGATYYYVVSSNTP
                     DGESPNSSEAVPSRLRTYLKFNESSGTAGFDATGNNWNGTLVNGPLRAAGKFGNAVDL
                     DGINDYVSLPAGVVNGLTSCTIAAWVYLDTVSNWSRIFDFGTGTNVNMFLTPRNGSTG
                     TVRFAITTSGAGGEQQINGSSALPTGVWTHVAVTLAGSTGILYVDGAEVGRNTAMSLT
                     PSSLGTTTQNYIGRSQYSADPYSDGRIDEFRIYTHALSGSEVAMLYAEQIPLDIPSVP
                     TGLSATAVSSGQIDLSWTVSSTATNYNIKCSMSSSGPYTLIASVSGTSYSDIGLSEST
                     VYYYVVSAVNSVGESADSEQAGAITHGLPPAAPSGLTAIAGDGSVILTWNANTEGDIA
                     GYNVYRSTTPEGEYTLLNGSLLSSPDFMDETVSHYTRYYYVVTAVDTNLLESPSSNPV
                     SVMPIDELAVVLSAADFESGFGEWVNITGEDFTDWTRNSGGTLTPYTGPSGGANGSTW
                     YVYLETSPGGANTAGNTAILQGPVIGGYNRVLMFYYHMYGAAIGTLNVDVFHNGVWQN
                     GIWSRSGQQHTSNSQPYTQAIVNLTGYSGPIQIRFRAVAAGGPTGDMAIDDIQVSGRP
                     VYGDMNGDNRLDMDDFVDFAGYWLQTHCGLDLDGDCKITLYEFAAFAENWLDSSSQ"
     assembly_gap    14115..14124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14125..>14675)
                     /locus_tag="PLE88_08950"
     CDS             complement(<14125..>14675)
                     /locus_tag="PLE88_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76628.1"
                     /translation="GGMMTQAMLGVYPDIFRAGVAFCGVPVGGAWTPVTRTAQQWGDI
                     VRNLNPGYTGPRPRIQLWHGTEDELVSYANQEESILQWTNVLGLSPTPTAAASLTMNG
                     RQWTRQIWQDAKGNTLVEALSEIGGTHSIGLLFDAHWVIPFLGLNIIGPHDPESSITI
                     PRGRPVLNAERTTFVADNGQLLRG"
BASE COUNT         3669 a         3955 c         3405 g         3636 t
ORIGIN      
        1 cgtctttgct gcggaggccg aaaccggctg gtgttagagg cacctcaacg acatcgccgg
       61 aataagaaat catatcttcg tttaatttca cataacgcag gtagggattg ccccaataca
      121 gatatgcctg gccgtcttca tcaataaaaa cagtcggatc gatatcaccc caactcccat
      181 cgttgcccac caaggggtgt cccagcgcat ctgtaaacgg ccctgccggg tgggttgcta
      241 ccgctacccc gattgctttc ccgccgactt tcatcttcat gggaacataa aaaaagaact
      301 tgccatttct gtaaacacat tgaccagccc aggcatcccc acttgcccaa ctgaagtcct
      361 tatatgacag cggtgatcca tgatcggtcc agtttaccat gtcggttgtc gaataacacc
      421 gccagtcatt catcgtaaag aaattattca ccaacacatc ttcatcatgg gtggtataaa
      481 gataaattct atcatgatga accaatggag ccggatcggc agtatattgg gtctgaataa
      541 taggattgtc ggcaagtcca attgtattca agtaaatgac tatcattatc gccatgtctg
      601 tgacatgttt ttttatttcg tgtttttcca tacaatattc acctttatta atacttttca
      661 tctttttaac atcagattgt cagatcaatt ccgtgccaaa cagaactaat tggcaggatg
      721 acttcccgaa gcattcagcc tgagcagacg aacgtcatat aacccacccg ccaatccccg
      781 gcttgcggtg aaccgaactg taaagcaacc gttttcagcg gtccgaagga tctcggcagg
      841 aaccggatag tgtttttcta taaaatcatg cggtttttcg ccgaacagct tttgagtagc
      901 gatctttgtg ccgttgacca agatatcaaa ttcgcgacct cgatcgtcac cggaataagt
      961 aacagataaa atgacttctt tggcgtcccg tgaatcgagg gtatattgaa tccagtaacc
     1021 atggcgccag cgccgaccgt tgtgaatccc cgtttccata ccttcaccct gaagatcatg
     1081 ctctacctca ggctgctgct cacctaccgc tatccagtcg atagttgccg cttcacgtac
     1141 ctgccgttgg cgttcctcag ctgcgagttt ttcccgtcgt gtcttaatct gttggggggt
     1201 agtaagttcc cagtacatct gatagcgacg ctcatgcagt ctgaaaaacg gcatcagcgg
     1261 caaacctgcc gggtcgtctg gttcaatgat atccttgata cgaaaatgca gcggtccggc
     1321 ggcacgatct tcaacaatat gcttaagcaa atcctcttcc gctgccagca aagtcggcac
     1381 cttgtcaagc ggaattaatg gaccatgcgc aacctgaccc atgcgacttc catcagcaaa
     1441 cagcccgtcc atgtgttcgg tgctgccggg ttttgctagt acaaccggac catacagaaa
     1501 agccacccag tcggagccgt caggcaagcg ctcagtgtgc agatgcatag gaagctccac
     1561 atctaccgtg tcaccgtcag cccattccct ccggatagcc gcataggaac aaggagttga
     1621 cttgacatca atcaatcgcc cgttaacctt gaccgcaaaa tcacccgctt tgacccagta
     1681 gggatggcga atattcagcg taaagacggt tggttttaga agttgaaaat gaagctgcgt
     1741 gcgaggctca tccgggaatt gcgtttgttg tcttagtacc acccccccca cattcagaga
     1801 aagcttcgaa ggaataaaaa gatttacata caactgatcc attctctgag cataaataaa
     1861 cgccccatac cgtccaggat tctccatccc cgtaccgaca cagcaccaga aggccacctc
     1921 cggctgagaa tatactctgt agtgatccgg tcggatagag gtgaaataaa catatccggg
     1981 tttttgagga tgaattgatg ccaagatatg attgtacaga gctcgctcgt aatagtctgc
     2041 atatgcagcc ctgggttctg ccttaaacag ttcctccgtc agtttgagca tattgtaggt
     2101 attgcaggat tcaggacctt cccggtgcgc aatcatgccg ctaaagtccg cggggttatt
     2161 aaaatgttcg ctgacgctgt tgccgccaaa ggccacagtg cgattctgcg tcacggtttc
     2221 ccagaaaaac tccgccccag tgtgcagttt ctcatcatga gtcagagccg caatccgctc
     2281 catgccgact atcttaggaa tctgggtatt ggcatggatg ccggtcagct tgtcctggcg
     2341 attctcgaga ggttcaaaaa cagaccgatg attgaagcgt ttggccagtt ccagatattt
     2401 tttatttccg gtgatcacat aaatatcagc aagcacatca ttcattccgc cgtattcgac
     2461 acccagcatt tgctgcatct gctggtcagt cagaccagat gttatctttt cacaccagtc
     2521 tgcgaaacca accaacaaat cacgcgactt tttgctgcct ccaacaagat aggcatcccg
     2581 aagtccggca aacattttat ggagattata ccacggggcc catctttttc ctataagctc
     2641 aacatgtccg tcggcgatac gggcccaaaa ttctttactt cccgatatcc ctccaaggta
     2701 accattgccg ttggcttttt ggacttgggc cagttcatca atcatatagt ccagacgacg
     2761 ctgaaattct ccgtccggat cagcacctga tgcaatcatc agtgatatcg ctgaaagata
     2821 atgtcccgcc gaatgccctt caagtcccat gctttcccag ttgccgtaag gctgcgcctt
     2881 gggcgacagt cccgcttcgc gacggaatgg tgccagcagt cgatcggcat gatgagccag
     2941 aagatactgc cgattggcct gaactgcctg agaaaaagga ctctccagca gccgcacatc
     3001 ccgcagcgta aataattgtg ccgacgcttg agtcactttt ggcggaagac taagaccttc
     3061 atcggtttgc atgataggtt ggatggttcc atcggcattg aagtaaagat attccgcaca
     3121 aacacttcgt cggcaatcgc cgccgggtat gccattgagg ctggtggagc catcgtggta
     3181 gaagaaatac cattgcccct tgaactcaat gaccgctgga tgaatggtaa aacctgcctt
     3241 gggagaaccg gttactaatc cgcgataggt ccatggacct gtgatttttt ctgctgtcgc
     3301 ataagcgacc ttttccggcg gattgccggg tacatcagcg gcatagatat tgtagtacaa
     3361 attcccgcgt ttgaacagcc aaggtgcttc cgcaacattt gggtgcggta ttttttggat
     3421 cggcccatcc agctcgatca tgctgggctt aagtctgacc agatagaaat caccgttgcc
     3481 ccacatcagc caagcggtac cgtcgtcgtc aataaacacg gcaggatcaa tatcggcgtt
     3541 gggacgactc gaatcggtag tcatatcatc ggtgatgagc ggagttccgc gggcatcctt
     3601 aaatggacca agcggactat caccaacagc cactccgata cagtgttcat tgttgttatt
     3661 gcgacgcaac gttgcataga aatagtagcg tccatccttc tcaatcatat ggcaggccca
     3721 tgcggtatca ggccgggcat acttgaaatc ctccggccgc ataatggggc catgtgacgt
     3781 ccataccttc atgtcttttg aggaataaca tcgccattcc ggcatagtaa aaaactgtcc
     3841 ctctttggcg ttatcgcgac cgacatagac atagaccgta tcgtcaacga ccagagccgc
     3901 cggatcagcc gtgaagacat cgcgaaaaat aggattttga ccaatacatg ggctaaccaa
     3961 tattgctgct gcaagaaatg tgattaattg catatatccg ttcatataca tagagctttt
     4021 ttccttattt gaatttccac cagttcaatt tcagcagtga tcctatgccc ccggtaaaaa
     4081 caaggtataa attatgcacg ccagcggcat ctttaaccga gcaccccacg gtcttccata
     4141 tttcccatcc cccggtgttg gaaactgcac aggttcccgc caggatccca tctggactgt
     4201 ccagacgaat ctcgatacaa ccgccttctg aaccggaaga gacccgagcc tcgaaaaaaa
     4261 actgcccccc tgccgaaatc cactccttta atcttaatat aatcaccgtt ttcgatgtta
     4321 cagacattca tccctccttc actgcagtgt tcggtcttaa cccccgatgc ccagcagatc
     4381 gtttcggctt ctacggtctg ataaggattg agaaagccga cctgaggtac gctcggtatc
     4441 gccgttccgt caccccacca aggaaccttc tgaatcgtgc cgtcggtgtt gtaggtcatt
     4501 gtttttacgc acactgaacg tctctccagt ttctttctgt gcccctgaag ggtgaaaaac
     4561 agttcataat ttaacccgaa aaggtaggtt gttcccttgt aatcaataat ccccggatgg
     4621 ttaccgtccg agtgagaatt cggatccatg atcgtccctt tgcacttcca tggacctgtg
     4681 ggactgtcgc tcatggcata agcgataccc tccggacagc agcgggacgc ccaagccagg
     4741 tattaatgat tgttcttctt ccacaaccat ggtccttctg tgtaagacgc ttcgggagga
     4801 gtgccggtaa aatcgatgga tgccgtctgg atgtcacccg aagtggaaat catgtcctca
     4861 ttaagcttga cataataaca aggaccgttg ccaccccaat acaggtaagc ctgtccgtca
     4921 tcatcgatga atgcagcagg gtcgtagtcc cctggattcc cgcgatagat tagaggtttg
     4981 tccagaggat caacaaatgg tccggtcgga ctttctgcca ccgctacacc aattgccatt
     5041 cgccccttga atatcgtagg acaataaaaa taaaacttgc cgtttctgac aatacaatgg
     5101 ggagcccacg cgctatgacc gtcggcccac ttgaaggtct tgtacggctc ggccactcca
     5161 gcaataatac catgatctgt ccaattgacc atatcctttg aactatatag cagccaatta
     5221 aacattttga aaccggtagc atcatcttcg tcatgggtgg tataaagata taccgtctca
     5281 tcatagacca tcggtgccgg atcggcggtg tattttgtct gaataatcgg attgtccgca
     5341 taacaaagca ttccaacagc cgttgcgaca gccattacca tggtcaaaat aagtttctgg
     5401 attcctgaaa tttctattgg tttctccata tttgtatttc ctgactcgat aatccgttga
     5461 caattcatgc cgccatcggc taatttaaaa cagcaaaaac ctgtacccct caactaaaat
     5521 tccaaagcta tgtcctgtcc tgtataaaca accgatttaa cagctctgga tgcatcactg
     5581 atacccgcat tttcttctga tacagcaaga acaatcttca gttggcgtgt tggcaccatc
     5641 cccggaaacg aacctttacg ggcaccaata tttagttttt tggctgcatc attccaaaca
     5701 agatcatagg tagcatattc acccttttcg tagttatagg tttcattgtc atcttcgtag
     5761 attgtgaact tcgcatcatc gcctgggtaa atgcgaattt cgtaaggggc atctggtttt
     5821 tcggtcgcat actgtataac cggacccatt ggtacaatcg aaccggcacg aatatacaac
     5881 ggcagaatat tcagcggaca tgctttttcg gccgtttttc ccccagtaag tttctcattc
     5941 gtccagaaat cataccaatc cgcaccagca ggcagatatg ttttcacgac attatctacc
     6001 gctgtctcat gttcacgttg attgcgaata tcgctggggg tgcgccagcc gagacggagg
     6061 atacgttcgc cgccggcctg gtagaattcc agtttcagcg gcacttgctg tcccttgctg
     6121 agcgtgactt tggcgctgcg gtagcgcgcg gcattctcgc gccaatcctc tatgattttt
     6181 ctcccatcta aataaagccg gaaaccgtca tccccttcga ccccgatttc atattcgccg
     6241 tcctgagggg ccaccagaaa tccttcccaa cgcgccgaga agttatttaa atcgttcagg
     6301 ccgccgggca gaacagccag cggcggtccg ggccagtcat gatcgacaac ctcgtcaatt
     6361 ccgttgccct ttggcgtatc aaagttgatg ccggcaaaat actgcacggc cagacccggt
     6421 ttgccgtcgg gagtccgcaa gaactccgtc ggcacggtcg gggcaggcgt actttctgta
     6481 cgatacatgg cgcgggtgac aggatgcacc agaaatgccg gcccgaacat aaaggcatcg
     6541 ctgatattgc ggactccggc atcatccggg aaatccatcg gcaaagcacg catcagcgtg
     6601 tatccttcac tggtaacttt ccagctgagg ctgtaaagat acggcatgag gcgataacgc
     6661 aggtgcgtaa tatccagcag cgcatcataa acttctgcat ccatctgtct gaagatatag
     6721 ggctcccgct caatattagt tccatgaata cgaaagattg gattgaaggc tccaaactga
     6781 taccatcgca agaagagttc gcgataggca ggattctgtt ccccgccggg gaagttgttg
     6841 acaaagaagc ctccgatatc ctgcgtccac cagggattgc cggccatggt cacatgaagt
     6901 ccgctggcca tctgagactt cagcatattc caactggccg acgaatcgcc tgaccaggag
     6961 gcagcagcgt aacgctgagc cccgcaccag gcagagcgag tcagtgtaaa cacacgctgg
     7021 ttgctgatgg cacgctgacc ttcataagtc cccttggtag tcataagcgt atagacattc
     7081 aagtaacgtg aaaaatcgcc cagagcattg cttcccagtc ttttgatatc acgcgcaact
     7141 tcgttgggat tccagcaggc cgtgccgacc tccacttcgg tgccgtccat ccaaagggca
     7201 tctacaccct tgtccagaag accgttcttc acatgcttaa agtaaatttc ccgtcccttt
     7261 tcactgaagg cgtcataaat gcgggctttc cgtgatatcc agtgcagcgg ctcaaatctc
     7321 agtttatact ggtccagttc atgggccagc ggggtatcat tacccacgga cggccagatg
     7381 gagatcatca gtttcatatt caggtcgtga attgccttta tcatgccctc gggatcggga
     7441 tagcgatctt tattccagat cataccgctc caggtgccat ctttatcgct tccccagtat
     7501 tgccagtctt gtacaatgta atccagcgga aattgctctt tgcggaaagt cctggccact
     7561 tcgacaatgc gctgctgggt ctgatagcgt tccttgctca taaacagacc gagggcctgt
     7621 ttgggataca gaggtgcatt ccccgtcaaa ttacggtacg cggcaattac atcgtccatc
     7681 gtgtcacctg caaagaaata gtaatccacg ccgccgggag cactttcagc ccaaagtgta
     7741 gcaccggctg catcgtcttt gaaccgcatg acggaataga tgtcccacat gattccatac
     7801 cgctcagagg atatgatcat gggaatcaca atgcccatat tggtctgaat cagcagcaat
     7861 tcctgccccc gtcggttgat ttggatggga gctgagtcga tgtagccaaa gccgtaccat
     7921 ccctcatcgg gcttcaaagt aaaggtattc gagacctcgt aggtcggggc accagaaatc
     7981 tcaacctgtt taatcatcgc atggtttacg gcacgctcgc aggtcaatag tttgccgctt
     8041 gcattcataa acctcagcgc tccagtttgt ttatcggcct taatctgaag tgtatccgat
     8101 acaatcgcaa cactgtctgc ggtctcatgg atcctgaatg gaacagccgc aggctggccc
     8161 acaactacga tacttggatg ctcccagtag ctgcggccga gattttcatt gacacgaact
     8221 gtggaagaac catagaaaat cacattcttg acagcatcgc cgacgtgaag ctgaatgccg
     8281 ttttccagag tgcagaaaga aaaatccttt ctatcggaac ccgcgatgga ctgtaaatgt
     8341 ccgaacttat ccaaaccgga aggagctgcc tgcagaacaa tactgaaaca cagaaacagg
     8401 aatgctgaaa aaaatttgat ttgcaccatt ttctccacaa cacttccttt tgtcataata
     8461 tctccaaatt ttatctgttt tgcaatatta aaaaattaaa tttacgtttt acggttaatg
     8521 cctaatcctg acagacgaag ctggaacatt taacccttct ggcagatcga tgcattctgg
     8581 acaattccct ctcacaggag tttcaagctg cttgagctgc agtttggatg ccccttgtcc
     8641 catttggtga cacggaattt tcttcgtcgt taacgacggt ttgataacat aacagaaata
     8701 tttatcatta acacacatca aattaacctg cttacatctg tgaatatcat aaagcatcat
     8761 ataatgcaaa aactttttct ctgcaatacg gtcattagcc gcaatcgcgg tagcctcttc
     8821 tttcaatgcc gctcggctca aaaaagtttg gcaccaaacg aaaaaagtgt catcgaagtt
     8881 gtccccatct gctggtcaga cttacctttg aatcaaggcg gaactgtttc gcacgggtaa
     8941 gcagttaacc ggctcttggg gatggacccc ggagggcgac cacgtcaaga gccgccgcga
     9001 tcggcagttc aggggcatca cgttcatgct tgggcacaaa attaaagaca tcctatccat
     9061 taatttgaaa aacgcacctt ggttccctct gttttcggat acgtgtcgcc gggccagctg
     9121 cacagcatat ttttctcaat gctgcctacc gccaaagttt tgcagcgaat cgtactttgg
     9181 gcggcaaaat tgagtcggta acctaattcg gccccagtac ggagtcgttg tttctgccgg
     9241 tttttaatgg cagaaaaagt gtcgggaaaa ttgccattca gcacatagct gactgtcccg
     9301 tcagtgatga cgcaacagca ggcaatatct cggactattg gtgctttcgg ctgactgatc
     9361 atggggttgc cggacaaaga gactatacga ggtctccaac aaaaaggaat ttccatcttc
     9421 gcgcttgttt cataattact ctttattttc tgaagttgac attattgaga tgaactatcc
     9481 agccagtttt cggcaaaggc ggcaaactca taaagcgtaa tcttgcagtc gccgtctaaa
     9541 tccaacccac aatgagtctg cagccaatag ccggcaaagt ccacaaaatc atccatgtcc
     9601 agacgattgt ccccgttcat gtcgccgtaa acggggcggc ctgaaacctg gatatcgtca
     9661 atcgccatat cacccgtcgg cccgccggca gctaccgccc ggaaacgaat ctggatcggg
     9721 ccgctgtaac ccgtcaggtt cacaatcgcc tgcgtataag gctggctatt ggaagtatgc
     9781 tgctggccgc tgcggctcca gatgccattc tgccacacgc cgttatggaa gacgtccaca
     9841 ttcagcgtcc caatagcagc accatacatg tggtaataga acataagcac gcgattatag
     9901 ccgccgatca ccgggccttg caggatggcc gtattgccgg cggtattggc accccccggt
     9961 gacgtctcaa ggtacacata ccaagtactg ccgttggccc cgcccgacgg acccgtatag
    10021 ggcgtcagcg tgccgccgga attgcgtgtc cagtccgtaa aatcctctcc tgtaatgttc
    10081 acccactcgc cgaatccgct ctcaaaatcc gccgcgctga gcaccaccgc cagctcatct
    10141 atcggcatca ccgacaccgg attggaagaa ggactttcca gcaagttggt atccacagcg
    10201 gtcaccacat aataataccg cgtgtaatgg ctgactgtct catccataaa gtccgggctg
    10261 ctcagcagcg aaccgttcag cagggtatat tcgccttccg gcgttgtgga tcggtaaaca
    10321 ttgtatccag caatatcgcc ttccgtattg gcattccagg ttagtattac cgaaccgtcg
    10381 ccggctattg ccgtcagacc ggaaggtgct gcaggaggta agccatgcgt tatcgcgcca
    10441 gcctgctcgg aatcagcact ttcaccgacg ctgttaacgg cactgaccac ataataatag
    10501 actgtcgatt cggacagccc tatatcgctg tagctggtcc ctgatacact ggcaatcagg
    10561 gtgtagggac cactgctgga cattgagcat ttgatgttgt agtttgtcgc agttgaagaa
    10621 accgtccaac tcagatcaat ctgtccgcta gatacggcag tggcactcag acctgtcggc
    10681 acagaaggaa tatcgagcgg aatctgctct gcatacagca ttgccacttc agagccgctt
    10741 aatgcatggg tgtagatccg aaactcatca attcgtccgt cgctatatgg atcagcagag
    10801 tattgagatc tgccgatata attctgcgtt gttgtgccca gtgaagaagg agtcaggctc
    10861 attgccgtgt tgcgcccaac ttcggccccg tcgacataca gaatccctgt actgcctgcc
    10921 agtgtgacag ccacatgcgt ccaaaccccg gttggaagcg ctgaacttcc gttgatttgc
    10981 tgctcaccgc ctgcaccgct ggttgtaata gcaaaacgca cagtccctgt cgaaccattg
    11041 cgcggggtca ggaacatgtt tacatttgtg ccggttccaa agtcgaatat gcgggaccaa
    11101 ttactgactg tatccagata aacccatgcg gcaatcgtac agcttgttaa tccgttaacc
    11161 acaccagccg gcaaagacac gtagtcattg atgccgtcca gatcaacggc attgccaaac
    11221 tttcctgctg cccgcagcgg accgttgacc agtgtgccgt tccagttgtt gccggtggca
    11281 tcaaagccgg ctgtgccgct ggattcgtta aatttcagat aagtccgcag tctggacgga
    11341 actgcctcac tgctgttggg gctttctcca tccggcgtgt tcgaactgac tacataataa
    11401 taagttgcac cccccgttat atttgtatca ctgaagcttg tcagagttat agtagaggca
    11461 atagttgtgt agggaccacc gctggcggtt gagcgtttaa tgttataact ggtcgccccc
    11521 aacgcagtcg tccaggtcag gttgacctga ctggaagtta tcatcgtcgc agataaattt
    11581 gccggcgcca caggtcccaa aggcagcggc cagccggggg ccgtcacatt gtcaaagacg
    11641 gcctgacaaa gactcgaatt attgtggcta gtgaccgcca aaccgatata gaccgtgctt
    11701 gccatactga tggtctgcga cgccccctgc tgcgtccagg taataccgtc aggagaatgg
    11761 tagccgctga aggtgctgcc gctgcgcact aacttaatcc agtagggcgc tgccagaccg
    11821 gtggcagcac tgttggcaga actgccgccg gtactggtgc ggtattgcca agtcacgccg
    11881 ttgcccggtg taacggcgat gaaggcattt tttgaattgg cattaaggct ttcgcgtatc
    11941 atcagaccgg ctttggccca tgaatcggta ttttgcactg aaacaacacg tgctatgatg
    12001 gtgcaatcac cattgaccgt caaataggta aagcggaatg catctgccgt accccagata
    12061 tcacttcccg atccattcac cacaaataca tcaccattaa aaatactgct gccggtcaat
    12121 gccgcagtac caatgtcctg agacttccag ggcgttacca cggccggcct gctgttgaga
    12181 ctttcccccg atagggtgtt ggcgctgact acataataat atactcttcc attgatcaca
    12241 tccgtatcgg tatagctggt tgccgttata ttagcagcaa taatagtata ggggccgctg
    12301 ctgttaaccg aacgtttaat attataactg gtcgcaccgg cagaggccgt ccagaataaa
    12361 tcaatttgtg tcgaggaaga ccactcggct gtcaaaccga caggaaatgc cggagcagac
    12421 ggacttaaaa ccgcccactt ctggtgggcc aagccggcat attcattgaa tacaatcgcg
    12481 gccccagcat caagagatgc attctcagct ccgcagtcca gacctgaatc ctcacggata
    12541 agacgataat agccatcccc cagcggttgg agaatgcagg tattccattg ccagttgtca
    12601 tatgctgaac gaacacggta ttgaccggcg ccctgatgct gaagactcca cttctgtgtc
    12661 gctgcgtcta taaaactgct ctggatcaca ttgcggttat tattctgtat cgccagaccg
    12721 ctgttttgat tgactatttt gtagacactg tcagtaacgg gaccaacagc agccggcgaa
    12781 agttcgatcc agttcagatt gaaaccaccg gtaacaattt caacacgcaa cttctgccgt
    12841 ccgtattcca gaaaaacctg tttggttatt gtagtccaat tctgatagcc tcccgtgttt
    12901 ggtatagtcc atctacccgt cttatccaga ttgtacgaaa cgacgcgtgc cgcacagccg
    12961 gtattcggtg aagcaacgcg taaccggagg ttgtaatagc ccgcttccgt tacaaagata
    13021 gtatattcaa gccattcacc atccatggtc cagccgatat tgtaaccgcc gccggaatca
    13081 gaagtaactt caatatccac gctttcggct gtccgatatt gcccaccctc gttggacatc
    13141 gtcatatcgt cataagccac acccggaccg ccaatatcaa aatcctcagc ctgaatacgt
    13201 aaagttccgc ttaaacctgt tgtagaccgg ggttggccgc cactgccgta tgaactccgg
    13261 atctgaggcc aaatcccgta gtcaggcatc caggacaagc ccgacctatc cacacgatcc
    13321 ttatagactt tgggtatcgt tatgcagtca gaaacacccc atggaggaat aaactggaag
    13381 gtcagccagg aaaccttgcg ccgttccatt tccgatgccg tatacagttc aaaaccgccg
    13441 ccttcttcac cccaaatacc tccggcaaac tccgtctgaa agcaagggta gccgaatccg
    13501 atcatggcgg cagcgaagtc tgaggttaaa ctgcttcccg aatagccgtg aatggccacc
    13561 gcctcgttgg tccaaatggc gtcgggattg ccaaaaacag ccgtattgaa cgcacgaaca
    13621 tctatcaaag cgtcgttggc tcctcctgat ccccacggca cagcataact gaacagcaag
    13681 accggcgtat cgggggcata cgaacggatg atattataag cggagatttc catatccact
    13741 gctcccggcg gattggtatg cgttaaataa ggaggccccc aagctaccgg ttcattctga
    13801 atttcaaaca gcacgtgcgt ctcattcgcg tagcgcggcg catagtatct ccagaaatct
    13861 tcaacataac tgcgattgta attcccattg ttggcgccat tgccgatcgt aagaatcaga
    13921 tataaaccca gattgcgtgt actctgcaca atactgtcaa tgcgggcggc cgcgtatccc
    13981 ggcgctgtcg aacccgggtt tggataattg atgtcaaaac attcgccata aagatgcacc
    14041 gcattaaaac cgaggttttt gatcttggcg atctcttcat atggtaccgc tccgctccat
    14101 tccgtggatg taaannnnnn nnnnccgcga agaagttggc cgttatcggc aacaaaggtc
    14161 gttcgttccg cgttaagcac cggacggccg cgcgggatag taatactgct ttcggggtca
    14221 tggggaccta tgatattcag acccagaaag gggatcaccc agtgggcatc aaacagcaat
    14281 ccaatgctgt gcgtaccgcc tatttctgac aatgcttcaa caagggtatt gcctttggca
    14341 tcctgccaga tctggcgggt ccactgtctg ccattcattg tcagcgacgc cgctgccgtt
    14401 ggtgttgggc ttaatcccag aacattggtc cactggagaa tggattcctc ctggttggca
    14461 taactgacaa gctcatcctc tgtgccatgc cagagctgga tgcgcgggcg gggtccggta
    14521 tatcccggat tgagattgcg aacgatgtcc ccccattgct gggctgtccg tgtaaccggt
    14581 gtccaagccc cgccaaccgg cacaccgcag aacgcaacac ctgctctgaa tatatcagga
    14641 tagacaccga gcatggcttg tgtcatcata ccgcc
//
LOCUS       DAOTBO010000073        14670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3450_length_14670_cov_5.437564, whole genome shotgun sequence.
ACCESSION   DAOTBO010000073 DAOTBO010000000
VERSION     DAOTBO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14670
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_14670_cov_5.437564"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLE88_08955"
     CDS             complement(<1..655)
                     /locus_tag="PLE88_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76629.1"
                     /translation="MARYYDRRAVSGQLVAFVPNSYAERTSRPIYGLVYLLGFLCLFL
                     VGTFLIQPAVLSQSLAEPQVRVVAFVWIQNIMEYLGFPPRTALIATPLVVVFILLILQ
                     ITSKAPWRIQWGDFLLMGGECILLSIPLIVLSLLLNRSNPPAGAAVNALPTPVQNQIL
                     VDMITGIGAGVFEEFIFRLVLICLLMLVFQDIFGLEKTPSIILSVAISAVLFSMHHHF
                     "
     gene            756..1424
                     /locus_tag="PLE88_08960"
     CDS             756..1424
                     /locus_tag="PLE88_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOM76630.1"
                     /translation="MAYTKLLDIYNRLYQRYGPQHWWPGQTPFEIIVGAILTQNTNWS
                     NVEKAIENLKKAGCLTPEQLHRLPTEQLASYIRPAGYFNIKASRLKHFLDWLFEKHDG
                     CLEKLAAMSPLTLRKQLLGIKGIGPETADSICLYAFGKPIFVVDAYTARMLGRHRLIE
                     DGSGYDQIQELFHSGLEKDVSLFNEFHALIVRLGKEHCKKQPVCKGCPLEDLPHQIEM
                     DPYD"
     gene            complement(1421..2707)
                     /locus_tag="PLE88_08965"
     CDS             complement(1421..2707)
                     /locus_tag="PLE88_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76631.1"
                     /translation="MGEQITLGESKYPKPVFPGASLREKKRANFGLDFGYWRNKGIDG
                     HQVRAIVDVLISRGYLKILLNPQLETVNGQPATVTIKDYSPIEEVRTGAAGASGVYNI
                     TKYVWVENTLSVTPYVYSDGSIGLKTDIKIGSRSKPEGVVQSPIITERSINVEENRIE
                     PGKSLIIGGMRKSERRSVVRGIPFFKDLPLLGILFSSKDFEEKGTEIIFILTPSISSG
                     GQDNTKVVEDIRRKYAAPQYQKGLTGTVAGPFTDAYLKVAEKRATEAEMARVQAEVDL
                     EQALQRAKTEKERADRAEAETQILRQKALQLQQQAQEVQAQAQAAAEQAQNTRSQIQT
                     SMQKVEELEAQRLQLNAQIQETLQEAQRLQQEAEDARQKAAQAEQQAQEARQQLQQAE
                     EQTNPAEPADQQPTPPDEQTPESPKEEAPAPAQPQP"
     gene            complement(3183..5018)
                     /locus_tag="PLE88_08970"
     CDS             complement(3183..5018)
                     /locus_tag="PLE88_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76632.1"
                     /translation="MSRLKICFFLLGLAIVLAGCGDFFAEKPTELESKAVIRDIARVR
                     ENPHIGNPLPQVYREGPKRLKVEDGVKLFYFTKYQPVGDLTFSDKDPARVKQVHGYGG
                     TIRDLGFKVSTNPITNQLIVHCATDEECDQVLAYLEKTDVPPIQVHIDCLILERFGDV
                     TKDWETTLLIENLLGEGITLGADKYPKPAFPGASLREAKRSTFGLDFGYWKNEGVPGH
                     QVRLLVDVLESRGYLKILMNPTIETVNGKTAQVQIRDNAPIELTVTEGGRTPYSVTDY
                     RWVADTLSVTPYVYADGSIGLKTDITFGSKSKPEGVVQTPIITERSVQVAENRIQPGK
                     SLIIGGMRKSENRSVVRGVPFLKDIPIIGILFSSKDFEENATEVIFILTPTISSGGTE
                     YKEMADMVRQKFQTPDYGSDFDAIVTDPLGTTAYSQVLEQQAEQAESEMIRLQVQTAH
                     AERQAQAARLRAQKAAALAREMESVAKESAAQIEHAKAQKAAAEAEQEAALKEAAAQQ
                     SRLTETQAAVEQVQAEAQRARQAAQEAQVALEQIRLKVQQFSQEAQKAHEEAQRIQQQ
                     LDELERQNAAEVPPSSEAGLPETESPAAEPQPSVPNEGVQGAQSQ"
     gene            5320..6669
                     /gene="rimO"
                     /locus_tag="PLE88_08975"
     CDS             5320..6669
                     /gene="rimO"
                     /locus_tag="PLE88_08975"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOM76633.1"
                     /translation="MVQKNKKSISVAFVALGCPKNMVDSEVMLAQIGQAGFVLSSDTD
                     ASDVVVINTCGFIEPAKQEALEAIRHAVRQKKKGRIRKVIVAGCLSERMGQALADEVN
                     GIDAVVGLSQRDQIASIIQNCFSPKHSPATKLFIEPSGSGIHDDRGRLLITPGHWAYL
                     RISEGCSRKCSFCTIPSIRGKFRSKPMEMILDEARELVSSGAVELSIIAQDSSFYGRD
                     IGLKDGLIQLIGRLETIEPLQWLRLMYLYPAAVDDTLIEAVAESRKVVRYIDMPIQHI
                     SNPILSSMRRADTKEHTIELVEKLRKAMPDIVLRTTVIVGYPGETEAQFEELLDFIRW
                     AGFDALGCFPFYPEAGTPAADLPGQLPDAVKQERLDTLMQTQQELVFRKMDAQLGRPL
                     TVLVDEAFDNQAVGRFYGQAPHIDSFCKIQNAAVEPGQFIQTRVIGRDGYDFIVHPL"
     gene            6735..7358
                     /locus_tag="PLE88_08980"
     CDS             6735..7358
                     /locus_tag="PLE88_08980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOM76634.1"
                     /translation="MKEKQKKGLIRYIPNALTVGRLVLTVVFLGLIVYAPKTSQPKPA
                     GLLMGAFVLFVITGLTDIIDGYLARRFEVTSRFGRVVDPLADKFLVCGAFFCFAWVNQ
                     PLMAGLGLSEWTLDFIRWGTAVVIFAREVVVQTLRHIAESRGVQFGAIWSGKLKMFLQ
                     SFGIGTVLIGWAYVSRPWGDWFTIITYVLMVLVTILSGIQSLTRRIK"
     gene            7388..9061
                     /gene="ettA"
                     /locus_tag="PLE88_08985"
     CDS             7388..9061
                     /gene="ettA"
                     /locus_tag="PLE88_08985"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOM76635.1"
                     /translation="MPPQFVFEMTNVSKRYGDKVVLRDISLSFYYGAKIGVVGENGAG
                     KSTLLKIMAGLDNDFHGQTKLAAKMKVRYVAQEPQLELDKTVRDHLQTAIKPILDMVQ
                     RFDAVSEKLGNPGPEDDMDKLLEEMGRLQDQIDACGGWETDRLIEIVSDAMVLPPDDA
                     VIRQLSGGERRRVALCMALLEKPDLLLLDEPTNHLDAETIQWLENALRDYPGTVIIVT
                     HDRYFLDNITKWILELENGYGLPFEGNYSSWLAQKAELLRIAEKKESQRQKTLLRELE
                     WINTSVKGRNQKNQARIHAYEELAARAEEAARSEVLIQIPSGPRLGQKVLAFHHVSKS
                     FGSEPLIEDCSFELPPNAIVGVIGPNGIGKTTLFRLITGEIAPDSGTIEIGPSVSIGY
                     VDQHRDALDGNRTIFEEISDGKDVITVGGRDVNARAYAAKFNFRGSQQQKKVSECSGG
                     ERNRIHLAKMLRRGGNLLLLDEPTNDLDINTMRVLEQSIIDFQGSAMVISHDRFFLDR
                     ICTHLLIFEGAGKIQWFVGNFAAYEEKVLAENPERIAHRRSKYKKLKLR"
     gene            complement(9290..10285)
                     /locus_tag="PLE88_08990"
     CDS             complement(9290..10285)
                     /locus_tag="PLE88_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76636.1"
                     /translation="MGKNIHMSMAADLWAPIQTGRQSVCRILIALAALAAALYAEHTA
                     PIPPLRVYAGGVAGLTDMLDAAGSRQFRAAGGGLYLHNSGWQTLRADQRKKILENFSD
                     CPVMLELGFGRDAGQVKAWAEVLKKDYLDLGVRPVVIASNAFDSNTRPTLEQWLAFAQ
                     GLRQAGLAETTKLLPTFEYQNYHLADQLAQNRITLSADFQNLLRAAGGIVIDSPAGYF
                     FSREQAYRDWVVDAIRWSHSQQLYALVIVSPHTSADQYSEDAERFVRYLCEHDAVPDA
                     FSVENYNPAAGPDWPNRVGREDQPDSQLGVAWFLLGDLLPRLLQPESKANDSTGQ"
     gene            complement(10391..11497)
                     /locus_tag="PLE88_08995"
     CDS             complement(10391..11497)
                     /locus_tag="PLE88_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOM76637.1"
                     /translation="MTVSLAKMRYFIFAGGGTGGHIYPNLAVSDALSRQCRDAQIIFF
                     CSSRDIDARILAPSGYDFLPLPAIGFSLRKCLLFFAQFVKSYYFVKQILTPIRREAVV
                     IGTGGFVTAPVVLAAKTLKIPIYLINVDSVPGKANRFLARFANKVFVQFAETAACFKA
                     SHAEVLGCPLRSGFQNPDRHKPFSDLALEENKKILLITGASSGSASINNTMISILPEL
                     NAFAAVWQVVHLTGLLHIEKVKEAVKGLDIAYHAVDYYDDMPNLLAAADVVVGRAGAV
                     SVAEYAAAGVPAVCLPYPYHKDRHQYKNAHVLADAGAAVIVDDNISNPSQTAHDLLTV
                     LKDLLADDAKRGQMAAAARKIGITDAAERILAAL"
     gene            complement(11494..12675)
                     /locus_tag="PLE88_09000"
     CDS             complement(11494..12675)
                     /locus_tag="PLE88_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOM76638.1"
                     /translation="MSITQSRIPYDRIIETVVLLLMAIGTVFVFSAGATVSGGYDLSQ
                     FYNVTTLKQLIFFPLAVGVMYAVSLIDYRRFSITDRPLGYSLTPWLSALTVGLLVAVL
                     FWGVERNYSKRWLDLAPGPLYISFQPSELAKWVMILFLGAYLERFSDQMHLFWRRFIP
                     VCLAAGLVVGLIVIEDFGTAAFIVMMTFLMLFIGGAKILHLLSPLPVVIPAFIAAVLA
                     SPTRINRIKAFLDPDNMQYQAKQSLIAISTGGLFGKGLGRGIIKYGHLPEDTTDFIFA
                     IIAEEMGLAACVVVIMLFVIFAIVGIAAVLRCRDRFGRLIGAGIVMAVTIQAAVNIGV
                     VTVVLPTKGIPLPFISAGGTSMLLTSAAVGVLLNMIRQTDQPQIDAEWNAVMGRQEGI
                     R"
     gene            12828..13172
                     /locus_tag="PLE88_09005"
     CDS             12828..13172
                     /locus_tag="PLE88_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76639.1"
                     /translation="MKKRIVAVLLAGMCIWGFCGCQESDSVMVRRARLVANENLKLQK
                     QLEEKERQIEQLKKETETLKNEREQAIASCGEVHLKLLEMVSESEKRNETLTVENEQL
                     KEELAKYKPAQP"
     gene            13225..>14670
                     /locus_tag="PLE88_09010"
     CDS             13225..>14670
                     /locus_tag="PLE88_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM76640.1"
                     /translation="MKNSSVLLSVLLSVIAGCANFHSAQVREKVELDKWQFCPDTTPE
                     GTTPIPPAAAEWAEVRIPHVFRLSGLHEQSAGWYRCMVPAAASEQDKRFYLFLEGAGS
                     VADVFVNGRHIGQHRGAFTAAAFDLTDALDLTGDNVLTIRVTNRDSEAGNCLSQSNLY
                     YTNGGLYRPVWLIKTSQVHIYPDMGSTGVYLTAKNITPQHADLEVRTYVKNCKSETAD
                     VVVRHTLIGPDGKTCGIFEVQAAVAAGAVQPITAVQPVKNPQLWDIHQPNLYTVQTEI
                     LADQKLSDMLTERTGFRTIAMDGDRFMLNGKELLARGVCKHHQGEHQWNAMTDADLLE
                     EWKGIIDLGCNTVRLAHYPHRRLEYHLADENGIAVWAENGLAGQKWDQGIKRETTPNA
                     DGERITREMVRQNWNHPSILFWSSGNETYQEVASHYADIIRQEDRSRLITYASAGEKP
                     VNVDFVAGNTYQGWYYAHYTDFAKLPDNAFIS"
BASE COUNT         3588 a         3941 c         3734 g         3407 t
ORIGIN      
        1 agaaatggtg atgcatgctg aaaagaacag cagaaatggc caccgacagt ataatggaag
       61 gcgttttttc caatccgaat atgtcctgaa ataccagcat cagcaggcaa atcagtacca
      121 gtcggaaaat gaactcttca aaaacaccgg ctccgatgcc ggtgatcata tccaccagaa
      181 tctggttctg tacaggagtc ggcaaagcat tcaccgcagc cccggcaggc ggattgctgc
      241 gattgagcag cagacttaga acaatcagcg gaatcgacag cagaatgcat tcgccgccca
      301 tcagcagaaa atccccccac tgaatccgcc atggagcttt cgaggttatc tgaagtatca
      361 gcagaataaa taccaccacc agcggcgttg caatcagcgc cgtacgcggc ggaaagccga
      421 gatactccat aatgttttga atccagacaa aagcaacaac gcgcacctgc ggctctgcca
      481 gcgactgcga gagcacagcc ggctgaatta aaaatgtccc gaccagaaac agacacaaaa
      541 agcccagcag atacaccagc ccataaatcg gccggctggt tcgctccgca tacgaattgg
      601 gaacaaatgc taccagctgt ccggatactg cccggcggtc ataataacgt gccacaaaaa
      661 ctcctgaatc tctgcattga tttcaactcc gccatcagta taatcaaact ggccgaaagt
      721 caaatggaaa ttggcgataa aaaacaggaa ccgcaatggc atatacgaag cttctggata
      781 tttataaccg gctttaccaa cggtatggtc cgcagcactg gtggccggga caaacgcctt
      841 ttgaaatcat tgtcggagcg attttaaccc agaatacgaa ctggtcgaac gtcgaaaaag
      901 ccattgaaaa tctcaaaaaa gccggctgtc tgacgcccga acaactgcat cggcttccga
      961 cagaacagct ggcatcttac atcagacccg ctggatattt caatataaag gcaagccgtc
     1021 tgaagcattt tttagactgg ctgttcgaaa aacacgatgg ttgtctggaa aagctggctg
     1081 caatgtcgcc gctgacattg cgcaaacagc ttctgggcat taaaggcatc ggtccggaaa
     1141 ccgccgattc gatatgtctt tatgcatttg gaaaaccaat ttttgtcgta gatgcctata
     1201 ctgcccgtat gctgggacga catcggttga ttgaagacgg cagcggatat gatcaaatac
     1261 aggaactgtt tcacagcggc cttgaaaaag atgtttcact ttttaacgag tttcatgccc
     1321 tgattgtccg ccttggaaag gaacactgca aaaaacaacc tgtctgcaag ggctgtccgc
     1381 tggaagacct gccgcatcaa atcgaaatgg acccttatga ttagggctgg ggctgtgcag
     1441 gtgcaggcgc ttcctcttta ggggattccg gcgtctgctc atcgggcggt gtcggctgct
     1501 ggtctgcagg ttcggcaggg ttggtctgct cttcggcttg ctgaagctgt tggcgtgctt
     1561 cctgtgcctg ctgttcggct tgtgcggctt tttgtcgggc atcttccgct tcctgctgca
     1621 gccgctgggc ttcttgaagt gtttcctgaa tttgggcatt cagctggaga cgctgcgcct
     1681 ccagttcttc aactttctgc atactcgtct gaatctggct tcgggtgttt tgggcttgtt
     1741 ctgcagcggc ttgcgcttgg gcctgcactt cttgggcctg ctgctggagc tgtaatgcct
     1801 tttggcggag tatctgcgtt tccgcttccg ctcggtctgc acgctccttt tccgtcttgg
     1861 cacgctgcag ggcctgctcc aaatccactt cggcctgaac acgcgccatt tctgcttcgg
     1921 tagctctttt ttcggctacc ttcagatagg catctgtgaa gggaccggct accgtgccgg
     1981 tcagtccctt ctggtactgc ggcgctgcat atttccgccg gatatcctct accaccttgg
     2041 tgttatcctg accgccggag gaaatgctgg gggtaagaat aaaaataatt tctgtccctt
     2101 tttcttcaaa atccttgctg gaaaacagaa tgcccagaag aggcaaatcc ttaaagaatg
     2161 gaatgccgcg caccaccgac cttcgttcac tcttgcgcat cccgccgata attaagcttt
     2221 tacccggctc aatgcggttt tcctcaacat taatcgaccg ctccgtaata atcggcgact
     2281 gaacaacccc ttcgggtttg gaccgcgaac ctattttgat atccgttttc aggccaatcg
     2341 aaccgtccga ataaacatag ggcgttacgc ttaaggtatt ttccacccac acatacttgg
     2401 tgatgttata aacaccgctg gcgccggcag caccggtccg gacttcttct atcggagaat
     2461 aatcctttat tgtaactgtt gccggctggc cgttgacggt ttccagctgc ggattcaaaa
     2521 gaatcttcag atagccccgt gaaatcagca catccacaat cgcccgcacc tgatggccgt
     2581 caatgccctt gtttcgccaa tacccgaaat ccaatccgaa attggctcgc tttttctctc
     2641 gcagcgaagc acccggaaaa accggtttgg gatattttga ttcacccagc gtaatctgct
     2701 cccccatcag attttctatc agaagagtgg tttcccaatc cgttgtaaca tccccgaacc
     2761 gctccagaat cagacaatcc acattgactt gcacaggagc aacatcaatc agctccaagt
     2821 attgctggac tgcatcggct tgtgcctgat ccttgcagta ggccacaatc tgattggtac
     2881 tggcattcac cgtcgatgac acatccagct gctgctgcag cagggctgcc agattgccgg
     2941 ccggatggtg cttggtaaaa tagaacagct tgacgccgtc cttgatggcc agccgcgaag
     3001 gcggctgacg ataaacctcc gggggcagat tgtcggtctg tacattttcg cgaacggcgc
     3061 tgagctcacg cagggtttct atggactcgg cttccgtcgg cttttgggca aaaaaatccc
     3121 cgcacccgcc gagcattccg gctatggcat acaggcaaat ggcctttatc caaattatac
     3181 ggttattgac tttgagcacc ttgaacacct tcattgggaa ctgacggctg cggttctgcc
     3241 gccggcgatt ctgtttccgg caagccggcc tctgaagatg gcgggacttc ggcagcgttc
     3301 tgccgttcca gttcatccag ctgctgctga atgcgctggg cttcttcgtg ggctttctgg
     3361 gcttcctgtg agaactgctg aactttcagg cgtatctgtt ccagcgccac ctgagcttcc
     3421 tgcgctgcct gacgcgcacg ctgggcctcg gcctggacct gctcgactgc cgcctgcgtc
     3481 tctgtcagcc ggctctgctg ggcagcggct tctttaaggg ccgcttcctg ttcggcctcc
     3541 gcagctgcct tctgcgcctt ggcatgctct atctgggcag cggattcctt ggccacgctt
     3601 tccatttccc gggcaagagc cgctgccttt tgtgcccgca gccgggcggc ctgtgcctgc
     3661 cgctcggcat gtgcggtttg aacctgaagc cgaatcatct ccgactcggc ctgttcggcc
     3721 tgctgctcaa gaacctgtga gtaggccgtg gtccccagcg gatcggttac aatcgcatca
     3781 aaatcagagc cgtaatccgg cgtttgaaac ttctgacgaa ccatatccgc catttccttg
     3841 tattccgtgc cgccggaaga gatggtaggc gttagaataa aaatgacctc tgtggcattt
     3901 tcttcaaaat ccttgctgga aaaaagtatc ccgataattg gtatatcctt caaaaacggc
     3961 acaccccgta caacagagcg gttttcgctt ttgcgcatcc cgccaataat cagcgatttg
     4021 cccggctgga ttcggttctc ggcaacctga accgaccgtt cggtgataat cggcgtctgc
     4081 acaactcctt ccggtttgga cttggaaccg aaggtaatat cggtcttcaa tccaatagaa
     4141 ccatccgcat aaacatacgg cgttacggac aaggtatccg ccacccatcg ataatcggtc
     4201 accgaataag gggttctgcc gccttcggta accgtcaatt caataggagc attgtcgcga
     4261 atctgcacct gagccgtttt gccattgact gtctcaatgg tcgggttcat caaaattttc
     4321 aggtagccgc gcgattcaag cacatccacc agcaggcgaa cctgatgtcc tggcacgcct
     4381 tcgtttttcc agtagccgaa atccaatcca aatgtggacc gtttcgcttc ccgcagcgag
     4441 gcccccggaa aagccggctt gggatactta tccgctccca gtgtaattcc ctcgcccagc
     4501 agattttcaa tcagcagcgt cgtctcccag tcttttgtaa catcaccgaa ccgctccaga
     4561 atcaggcagt caatatgcac ctgtatcggc ggcacatcgg tcttttccaa atatgccagt
     4621 acttggtcgc attcttcatc tgtcgcacaa tgcacaatca gctgattggt tatcggattc
     4681 gtactgacct taaagcccaa atcccggatg gtcccgccgt atccgtgaac ctgtttgacg
     4741 cgggctgggt ctttatctga aaaagtcaaa tcacccaccg gctggtattt tgtaaagtaa
     4801 aacagcttca ccccgtcttc cactttgagc cgtttgggac cttcccgata gacctgaggc
     4861 aagggattgc cgatatgggg attttcccgt acccgcgcta tatcccgtat gaccgctttg
     4921 gattccaact cggtgggctt ttcagcaaaa aaatccccgc agccggccaa aacaatagcc
     4981 agcccgagaa ggaaaaagca aatttttaaa cgggacattc cggacattaa tcaatccaaa
     5041 aacataataa acaccctttt ttcatccgcc ccttcatcag gtacgctttt caatattgtt
     5101 tcttcggctc atgaaacgtt ttttctaaaa tcaaacagca gtgaaaatac agataaccaa
     5161 tctttttgcc cctggtatct ataatcatcc tttttattgc ctaaaacgaa tgattcgggc
     5221 agtctattgc agaccaacag gcaaatttct ccacacaatg cgtcgtgttt tataaagtga
     5281 tggatttttg agtccgttta tgttattttt ctttgtctaa tggtccaaaa aaataaaaaa
     5341 tctatttccg tggcttttgt cgctctgggg tgtcccaaaa acatggtcga cagcgaggtt
     5401 atgctcgccc agattggaca ggccggcttt gtgcttagtt ctgatacgga tgcctccgat
     5461 gtggtcgtta tcaatacctg cggttttatt gagccggcca aacaggaagc gctggaggcc
     5521 atccggcatg ccgtccggca aaagaaaaaa ggccggattc gcaaagtcat tgtggccggc
     5581 tgcctttctg agcgtatggg gcaggcgctg gccgacgaag tcaacggcat cgatgctgtt
     5641 gtcggtcttt cgcagcggga ccaaatcgcc tcaatcattc aaaactgctt tagtcctaaa
     5701 cattctccgg ccacaaaact ttttattgag ccgtccggca gcgggattca cgatgaccgg
     5761 ggacgcctgc tgataactcc gggacattgg gcgtatctgc gcatcagtga aggatgcagc
     5821 cgaaaatgct ccttctgtac tattccctcg attcgcggaa aatttcgcag taaacccatg
     5881 gaaatgatcc tcgatgaggc ccgtgaactg gtttccagcg gtgctgtgga actgagcatt
     5941 atcgcacagg acagcagttt ctatggtcgg gatattggac ttaaagacgg attaatacag
     6001 ctgattggcc ggcttgaaac aatcgagccc cttcaatggc tgcggctgat gtatctctat
     6061 ccggcagctg tggatgatac tctgattgaa gccgttgctg aaagcaggaa agtggtccgc
     6121 tacatcgata tgccgattca gcatatcagc aatcccatcc tttcttccat gcgtcgagcc
     6181 gataccaaag aacacactat agagctggtg gaaaaactgc gaaaggcaat gccggatatt
     6241 gtgctgcgaa ccaccgttat cgtcggctat cccggtgaaa ccgaggccca attcgaagaa
     6301 ctgctggatt ttatccggtg ggctggattt gatgcgctgg gctgttttcc cttttatcct
     6361 gaggcaggaa cccctgctgc ggatctgccc ggtcagctgc cggatgccgt caaacaggag
     6421 cgtctggata ccctgatgca aacccagcaa gagcttgtat ttagaaaaat ggacgcccag
     6481 ctcggacgcc cgctgaccgt tttggttgat gaggccttcg acaatcaagc ggtcggacgc
     6541 ttttacggcc aagcccccca cattgacagc ttctgcaaaa tccagaatgc cgccgtcgaa
     6601 ccggggcaat tcatccaaac ccgcgttatc ggccgtgacg gttatgattt tattgtccac
     6661 ccgctatagt ttggcttctt gagctttgga tttacccttg ctctgacagt gcaataccgc
     6721 tataattccg cccgatgaag gaaaaacaga aaaaaggatt gattcgttat attcccaacg
     6781 cactgacggt tggacggctg gttctgacgg tggtatttct ggggctgatt gtctatgcgc
     6841 caaagaccag tcagcccaaa ccggccggcc tgctgatggg ggcgtttgtc ctgtttgtca
     6901 tcacaggcct gacagatatt atcgacggat atctggcgcg ccgatttgag gtaaccagcc
     6961 gttttggacg ggtggtggac ccgttagctg acaaatttct tgtctgcggg gcattctttt
     7021 gctttgcttg ggtcaatcag cccctgatgg ccggcttggg cctttcagaa tggactctcg
     7081 actttatccg ctgggggaca gccgttgtta tttttgcccg ggaggttgtt gttcaaacac
     7141 tgcgtcatat cgccgaaagc cgcggcgtgc agttcggagc tatctggtcg ggcaaactga
     7201 agatgtttct tcagtccttc ggaatcggga ctgttttaat cggctgggcg tatgtctccc
     7261 gcccatgggg cgattggttt accataatca cctatgttct gatggtgctt gttacaatcc
     7321 tgtcgggtat tcagtcccta acccgacgga ttaaatagca aatcatcctc ttgtccaagg
     7381 aaatactatg cctccccaat ttgtttttga aatgaccaat gtatccaaac gctacggcga
     7441 caaggtcgtt ctgcgggata tttcgctgtc gttctattac ggcgccaaaa tcggcgttgt
     7501 gggcgaaaat ggagccggca aaagcaccct gctgaaaatt atggccgggc ttgacaatga
     7561 ttttcacggc cagaccaaac tggctgccaa aatgaaagtc cgctacgtgg cccaggaacc
     7621 gcagctggaa ctggataaaa ccgtacggga ccatctccag acggcgatta aacccattct
     7681 ggacatggtc cagcggtttg atgcggtttc agaaaaactc ggcaatcccg gccccgagga
     7741 cgatatggac aagctgctgg aagagatggg gcggctgcag gatcaaattg acgcctgcgg
     7801 gggctgggaa accgaccggt taatagagat cgtttccgat gccatggttc tgcccccgga
     7861 tgatgcggtt atccgccagc tttccggcgg cgagcgccgc cgcgtggcct tgtgtatggc
     7921 gctgctggaa aagccggatt tgctgcttct ggacgaacca accaatcact tggatgctga
     7981 aacaattcaa tggctcgaaa atgccctgcg ggactatccc ggcaccgtga ttattgttac
     8041 gcacgaccgc tattttctgg acaacattac caaatggata ctcgagctgg aaaacggata
     8101 tggtctgccg tttgaaggca attattcctc gtggctggcc cagaaggccg aactgctgcg
     8161 cattgccgaa aagaaggaaa gccagcggca gaaaaccctg ctgcgcgaac tggaatggat
     8221 taatacttcg gtcaagggac gcaaccagaa aaatcaggca cgcatccatg cctatgaaga
     8281 attggccgcc cgcgctgaag aagccgcccg cagcgaggtt ttaatacaaa ttccgtcggg
     8341 gccgcgattg gggcagaagg tgctggcgtt tcatcatgtc agcaagtcgt tcggctcgga
     8401 accgctgatt gaggattgtt cgtttgagct gccgcccaat gccatcgtcg gcgttatcgg
     8461 tccaaacggc atcggcaaga caaccctgtt tcgcctgatt accggcgaaa ttgcccccga
     8521 cagcggcacc attgaaatcg gaccgtccgt gtcaatcggc tatgtggacc agcaccgtga
     8581 tgctctggac ggcaaccgaa cgatctttga ggagatatcc gacggcaaag atgtcatcac
     8641 tgtcggcggc agagatgtca acgcgcgggc gtatgcagcc aagttcaact ttcgcggaag
     8701 ccagcagcag aagaaagtat ccgaatgctc cggcggcgag cgaaaccgca tccatctggc
     8761 taaaatgctc cgccgcggag gaaacctgct tctgctggac gagccaacca acgatctgga
     8821 tataaacacc atgcgggtat tggagcagtc tattatcgac tttcagggct ccgcaatggt
     8881 catcagccac gaccggtttt tcttagaccg catctgcacc catcttttaa tctttgaagg
     8941 cgccggcaag atacagtggt ttgtaggcaa ttttgcagct tacgaagaaa aggttctggc
     9001 cgaaaacccc gaacgcatcg cccaccgccg cagcaaatac aagaaactca aactgcggtg
     9061 attgcttata ggcactcgct taagccaagc agcgccgccg gcaaaatgga gatacattag
     9121 cgcaaacgct tttacagcga tgtcccttgg aaggcttgtt tgttttcaag cgttccgccg
     9181 atggtattta tgcgcctttt actggatacg atttggacga taaaaaaagc aaaacggcag
     9241 attgccccgt cagaaacatc tgctttgccg ggatacactg tacgggggct tattgaccgg
     9301 ttgaatcatt tgccttggac tccggctgca ggaggcgcgg cagcaggtcg cccagcagaa
     9361 accaagccac acccagctgg gagtccggct ggtcttcccg tccgacacgg ttgggccaat
     9421 ccggcccggc ggcgggattg tagttctcca cagagaatgc gtcaggcaca gcatcatgct
     9481 cacaaagata tcgcacaaaa cgttcggcgt cctcgctgta ttggtcggct gaggtatgcg
     9541 gcgagacgat gacaagagcg taaagctgct ggctgtgcga ccaccgaatg gcatccacca
     9601 cccagtcgcg gtaggcctgc tcgcgggaaa agaaatagcc cgccggggaa tcgatgacaa
     9661 tgccccctgc cgcacgcagc agattttgaa agtcggccga taaagtgatc ctgttttgag
     9721 ccagttggtc ggcgaggtga taattctgat actcaaatgt cggcagcaat ttcgtcgttt
     9781 cagcaagccc tgcctggcga aggccttggg cgaaagcaag ccactgttca agggtaggac
     9841 gggtattgct gtcaaaggcg ttggaggcga tgacaacggg tcggacgccc aagtccaaat
     9901 agtctttctt caaaacttcg gcccatgcct tcacctgccc ggcgtcgcgg ccaaatccca
     9961 gttccagcat gaccgggcaa tcggagaagt tttcgaggat ttttttccgc tggtccgccc
    10021 ggagtgtctg ccagccgctg ttgtgcagat acagcccccc gcctgccgcg cgaaattgac
    10081 gcgagccggc ggcatcaagc atatcggtca gacccgcaac cccgccggcg taaacacgca
    10141 ggggcggaat cggggcggta tgttcggcat agagtgcagc agccagcgcg gcaagggcga
    10201 taagaatccg gcagactgac tgccggcctg tctgtatagg tgcccacagg tcagcagcca
    10261 tcgacatgtg aatgtttttt cccattttgc cgtccgcaga agggcagaag aacggttgca
    10321 aaccggagca tatcaatacg cagcctgctt gtcaacagca caagacgctt aagaaacgca
    10381 aagtcattgt ttagagagcc gccagaatgc gttcggcggc atcggtaatg ccgattttcc
    10441 gtgcagcggc agccatttgt ccgcgtttgg catcatctgc cagcaggtct ttaagcaccg
    10501 ttagcaaatc gtgtgccgtt tgcgaaggat tgctgatatt gtcatccacg ataaccgccg
    10561 caccggcatc tgcaagaacg tgggcgtttt tgtactggtg gcggtcctta tgatacgggt
    10621 acggcaggca cacagccggt acccccgcgg cggcatactc ggccacgctg accgcaccgg
    10681 ccctgccgac gaccacatcc gcagcagcca gcaggttggg catatcatca tagtaatcga
    10741 ccgcatggta ggcgatatcc agccccttga cggcctcttt gaccttctca atatgcagca
    10801 gacccgtcag atgcacgacc tgccaaacag cagcaaaggc atttaattcg ggcagaatag
    10861 aaatcattgt attgttgatg ctggctgaac cgctcgatgc accggttatc agcaggattt
    10921 ttttgttctc ctccagcgca aggtccgaaa agggtttgtg ccggtcggga ttttgaaagc
    10981 ccgacctcag gggacatccc aacacctcag catggctggc cttgaaacaa gccgctgttt
    11041 ctgcaaactg cacaaacacc ttattggcaa atcgggcaag gaatcggttg gccttgccgg
    11101 gaacgctgtc gacgtttatt aaataaatcg ggattttcag cgtctttgcc gccagcacta
    11161 ctggggctgt tacaaacccg ccggtaccga taacgactgc ttccctgcgg ataggagtta
    11221 aaatctgttt gacaaaataa tagctcttta caaattgagc aaaaaacagg agacattttc
    11281 gcagactaaa accaattgcc ggcagcggca gaaagtcata tccgctggga gccagaatgc
    11341 gggcatcaat atcccggctg ctgcaaaaaa aaatgatctg ggcatctctg cattgacgac
    11401 ttaacgcatc agaaacagcc aaattaggat aaatatggcc gccggttccg ccgcctgcaa
    11461 aaataaaata ccgcatttta gccaatgaga cggtcatctg ataccctcct gccgtcccat
    11521 aacagcattc cattcagcat cgatttgcgg ctggtctgtc tggcgaatca tattgagcaa
    11581 aactcccact gctgccgaag tcaaaagcat acttgtcccg ccggcactga taaacggcag
    11641 cggaatgccc tttgtaggca gtactacggt aacaactcct atattgacgg cggcctgaat
    11701 cgttacagcc atcacaattc cagcaccaat cagccgcccg aaccggtcgc ggcagcgcaa
    11761 gaccgctgca atgcctacaa tcgcaaaaat cacaaacagc attatcacca ccacgcaagc
    11821 ggccagcccc atctcttcag caatgatggc aaaaataaag tcggtcgtgt cctccggcag
    11881 atgcccatat ttgatgattc ccctgcccag ccccttgccg aacagaccgc cggtgctgat
    11941 ggcaatcagg gactgtttgg cctgatactg catattgtct ggatcaagaa acgccttaat
    12001 gcggttgatg cgggtcgggc ttgccaaaac agctgcaata aaagccggaa taacaaccgg
    12061 cagaggcgac agcagatgca ggatctttgc tccgccgatg aaaagcatca gaaaagtcat
    12121 cattacaatg aaagcggctg ttccgaaatc ttcaataaca atcagtccaa ccaccaaacc
    12181 ggcagcaagg cagacgggga taaacctccg ccaaaacaga tgcatttggt cggaaaaccg
    12241 ctccaaatac gcccccaaaa agagaatcat aacccatttt gcaagttcgg acggctgaaa
    12301 actgatatac agcggcccgg gcgccaaatc cagccagcgt ttggaataat tgcgctcgac
    12361 cccccaaaac agcactgcaa ccagcagtcc tactgtcagc gccgacagcc aaggtgtcag
    12421 cgaataaccg agcggacggt cggtgatgct gaacctgcgg taatcgatca gggataccgc
    12481 atacatcacc cccacagcca gagggaaaaa aatcagctgc ttaagtgtcg taacattgta
    12541 aaactggctt aaatcgtagc cgccgctgac cgtcgctccg gcggaaaata caaaaaccgt
    12601 cccgatggcc atcagcagca gcacaacagt ttcaataatg cggtcgtatg gtattctcga
    12661 ctgtgtaatt gacataggaa taatgtcggc acaactgccg actttacaac aaaaaaacac
    12721 atttcacgca ggcaagcttt tcttgcctgc tgaggaaaag gtgttgattc tatcgctttt
    12781 ttccttaaaa tcaatttgta aaagatcaat taccaatgga ggcctatatg aaaaaacgca
    12841 ttgtggctgt tctgcttgca gggatgtgca tctggggatt ttgcgggtgt caggagtccg
    12901 attccgtaat ggttcgccgg gcacggctgg ttgccaatga aaatctcaag cttcaaaagc
    12961 aattggaaga aaaagaacgc cagattgagc agttaaaaaa agaaaccgaa acgcttaaaa
    13021 atgagcggga acaggcaatc gccagctgcg gggaagtgca tctcaaactt ctggaaatgg
    13081 tctccgaatc agaaaaacgc aatgagaccc tgacagtcga aaatgagcag ctcaaagagg
    13141 agcttgccaa gtacaagccg gcccagcctt aaaaacagtc cgcacagttt aaaaagcggg
    13201 tgaatcttgc aggagatata gattatgaag aactcatctg tcttgttatc agttctgttg
    13261 tctgttattg caggatgtgc caattttcac tccgctcagg tccgtgaaaa agtggagctt
    13321 gataaatggc aattctgccc ggatacaacc cctgaaggaa caacgccgat tcctcctgca
    13381 gcagcagaat gggcagaggt tcgcatcccc catgtcttcc gcctgtctgg tctgcatgag
    13441 caatcggccg gctggtaccg gtgcatggtg ccggcagcag catccgaaca ggacaagcgt
    13501 ttttatctgt ttctcgaagg cgccggttcc gtagccgatg tctttgtcaa cggccggcat
    13561 atcggacagc atcgcggggc ttttaccgcc gcagcgtttg acctgacgga tgcgctggat
    13621 ttgactgggg ataatgtcct gaccattcgc gttaccaatc gggattcaga agcaggcaat
    13681 tgtctgtcgc aatccaatct ctattataca aacggggggc tgtatcgccc tgtatggctg
    13741 attaagacca gccaagtcca tatctatccc gatatgggtt ctaccggcgt atatctgacg
    13801 gccaaaaata ttacgccgca gcatgcagac ctcgaagtca ggacctatgt aaaaaactgc
    13861 aaaagcgaaa cggccgatgt tgttgtgcgg cacacgctga ttggcccgga tggaaaaacc
    13921 tgcggtatct ttgaagtcca agccgctgtc gccgccggtg ccgtccagcc gataaccgcc
    13981 gtccagccgg ttaaaaatcc ccagttgtgg gatattcatc agcccaatct gtacacagtg
    14041 cagacggaaa ttctggccga tcagaagctg tccgacatgc tgacagaacg gacgggcttt
    14101 cgcacgattg cgatggatgg cgaccgcttt atgctcaacg gcaaagaact gctggcacgc
    14161 ggcgtctgca agcaccatca gggcgaacat cagtggaatg cgatgaccga tgcggatttg
    14221 ctcgaagaat ggaaaggaat aatcgatctg ggatgcaata ccgtacggct ggcacattat
    14281 ccgcaccgcc ggctggagta ccatctggcc gatgaaaacg gcattgccgt ctgggcggaa
    14341 aacggcttgg ccggtcaaaa atgggatcag ggcatcaagc gtgaaacaac ccccaatgcc
    14401 gacggcgaac gcatcacccg cgaaatggtc cgtcagaact ggaaccatcc gagcattctt
    14461 ttctggagca gcggcaatga aacctatcag gaggtggctt cgcattatgc cgatattatt
    14521 cgtcaggaag accgctcccg tctgattacc tatgcgtcag caggagaaaa accggtcaat
    14581 gtggatttcg tggccggcaa tacctatcaa gggtggtatt atgcccatta caccgacttt
    14641 gccaaactgc cggacaatgc gtttatatct
//
LOCUS       DAOTBO010000074        14334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3558_length_14334_cov_5.428041, whole genome shotgun sequence.
ACCESSION   DAOTBO010000074 DAOTBO010000000
VERSION     DAOTBO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 14334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14334
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3558_length_14334_cov_5.428041"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLE88_09015"
     CDS             <1..958
                     /locus_tag="PLE88_09015"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOM76641.1"
                     /translation="KALGFRIPKYAHMSVTVSDTGGKLSKRERPQALRNAMAGMKHLD
                     KAAAAKAGGISLDQLESFLNGDSTPDMPAIDAMAQYLGVHLPEINVVDFFRSGYLPET
                     MVNFLALLGWNPGDGREIMTRRQLIESFDLTRVTKANSLFDRSKLLSFNTEHIKLTPI
                     DTLVGHFRSYLEAVKSPVAKADDAMLARIIRLNEGARTLAQMEMKSRFAFIPNEEVVY
                     DPESVKKVLLKGDGLAILQEIGQELGALTELTAENVETLLRDLAARRQVGLGKVAQPL
                     RVALCGTTVSLPIFDAVQMLGIANTLKRIKQTVSKFGSAKEG"
     gene            970..1875
                     /locus_tag="PLE88_09020"
     CDS             970..1875
                     /locus_tag="PLE88_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOM76642.1"
                     /translation="MSLLVTGSVGIDTVQTPFGLNRDCIGGSAVYFTMAAGFFTDVKF
                     IGVVGDDCPFNLEELFSGKKVDLTGLEVRRGSRTFRWHGTYEGDMNQARTDAVELNVL
                     AERPPAVPDSYRDCEYVFLANTAPLLQMELLAQLGNPEFVAADTMNLWIETANQDLRR
                     LLEQIDMLVLNENEAKLLTGYHNLISAAKAIAAMGPRIVIIKKGQYGSMMYEKNGSIF
                     LLPAYPTEVVIDPTGAGDAFAGGLMGYLAQVQTVDLTSLQNAMAYGTVMASFTIGDFS
                     IYGIRSATQEKIEQRFDMLRKVTQF"
     gene            1894..2454
                     /gene="thpR"
                     /locus_tag="PLE88_09025"
     CDS             1894..2454
                     /gene="thpR"
                     /locus_tag="PLE88_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOM76643.1"
                     /translation="MRLFAAIDIDPQVRGRIEAVQKQLARQLNLTGRDVKWVRPDQIH
                     LTLKFLGEVRDAAVGQVCDAVIRTAGRFSGFDFEVRGTGVFGRPARIVWAGGAPCPPL
                     MDLQAALEDEFSRLGWQKESRPFTCHLTLCRVKSAAAGYRLAEAIEAYRDEPFGSVSV
                     SSIVLYHSQLDSAGPQYSVVCTGALK"
     gene            2514..3596
                     /gene="recA"
                     /locus_tag="PLE88_09030"
     CDS             2514..3596
                     /gene="recA"
                     /locus_tag="PLE88_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOM76644.1"
                     /translation="MAKAKQKTADKETKTVDSARSDALDRAIAQIEKQYGVGSIMKMD
                     ESQHPKIEGISTGALSLDIALGGAGIPRGRIVEFYGPESSGKTTLALHVIANAQKAGG
                     VAAFIDAEHALDTTWAKKLGVDISGLLISQPDTGEQALDIAEMLVKSNAVDIIVIDSV
                     AALVPAAELQGEMGQSHVGLQARLMSQALRKLTGVIAKSKCCLIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRVDVRRISTIKAANDEAIGNRVRARVVKNKVAPPFRAAEFD
                     IMFDSGINYVGDLLDLAVECGLVNKSGAWFTCGDLKLGQGRDKAMQYLKDNPALLCEL
                     KDKVLANRGLIQCRDSGKEASDETSE"
     gene            3664..6378
                     /gene="alaS"
                     /locus_tag="PLE88_09035"
     CDS             3664..6378
                     /gene="alaS"
                     /locus_tag="PLE88_09035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOM76645.1"
                     /translation="MVLSSKDIRSGFIEFFKGHNHLFIPSWPVVPIGDETLLFTNAGM
                     NQFKEYFLGHRTPSFKRAVNSQKCIRVSGKHNDLEEVGLDTYHHTFFEMLGNWSFDDY
                     FKAESIEWAWQLLTGVYGIAPDRLWATVFAGDKEDNAAADTEAAELWTKLTPLPKERV
                     LFCGRKDNFWEMGNTGPCGPCSEIHIDLGPDRCDMKHIPGHRCAVNAGCSRFIELWNL
                     VFIQFNRKPDGRLERLSANYVDTGAGLERITAVLQNKRSNYDTDLFLPIIQSVEQIAG
                     RHYTSQLGNKTDNAFRVIADHIRTLTFAIADGVNPSNEGRGYVMRRLLRRACRFGRSL
                     NLHEPFMYKLVDVVVDNMGQAFPEIRERREFVASVIQAEEASFGRTLDRGLEIFANAA
                     KDAKNGIISGQDAFQLYDTYGFPLDLTQLMAREQGLTVDIDEFERLMTQQRQRARAAQ
                     KTGSLAATLTGVELPETDDSLKYETETCRAKLLGWINDDGFTTKGELTDTEHPAALVL
                     DKTCFYAESGGQVGDCGIIKTEDAEFCVETTDKIADCVLHRGRLLSGSLWVGDQVQAC
                     VDKSRLASRKNHTATHILQWALQTVLGQTVKQQGSLVCPDYLRFDFTWPKALTKEQIQ
                     QVEALVQDKIEAALPVTTAVMDIDRARQLGAMALFGEKYGQQVRVLAIGASDKDHIKD
                     AFSKEFCGGTHVSNTAEIGGFAIQKEESISAGVRRITALTGPGLMRYLLDRSRIADEL
                     VELLKVPADQIVQRIGKLVDENKTLKKELKSGVGKSASDAFGAADKLLESAQKAGEAF
                     VVVGQLPSVDVEQARAAVDSIKKKARCAAIVFGMVTGDNKVMLLAGVTEDLIQKGLKA
                     GDIVKEIAPIVGGGGGGKPNMAQAGGKEPDKLDQALERAKQIITAHLG"
     gene            6784..7326
                     /locus_tag="PLE88_09040"
     CDS             6784..7326
                     /locus_tag="PLE88_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76646.1"
                     /translation="MKKFAVLMLLISVVTAQASLKIYVKWDEGPYGSMYIDYPDSKLT
                     INPSDVITIGIMDILGQTQPGALALGISMGPGSLDASGMKPLAGVSAALTNDPDSAAQ
                     YGLLSPYVALEIGPNMTGMLIRDLLFHCDGPGDVTIVLVDENGMIVDSQVIHQPEPMT
                     LGLLGLGGLSLVMSRKRRAA"
     gene            7496..7999
                     /locus_tag="PLE88_09045"
     CDS             7496..7999
                     /locus_tag="PLE88_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76647.1"
                     /translation="MMKRILMMLLLCGLANASLYITVNGVINPPDSTVTIKPSDTLWI
                     GIWDDGQTAPGLLAMGLTMGPGSLDASKMSVQAGVLAAVSDDAGKAGDLGLQSPFIAM
                     EIGQIQPGTLISEVLFHCDGPGDVTIALVDDNGIVVDTQVIHQVPEPFTLATLALGSL
                     LLRRRIA"
     gene            8392..11271
                     /locus_tag="PLE88_09050"
     CDS             8392..11271
                     /locus_tag="PLE88_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOM76648.1"
                     /translation="MKRELYFLLSVLFSARLVGGTLYVPTQYGSIQSAINAAAEKDVI
                     IVQPGTYVENINFLGKKITVQSADPDDPNVVAATIIDGSAPDDPAQASVVTFSSGEDN
                     ESILTGFTIRGGTGSWIAVAWKYQGLRWNRCGGGVVCYNLSAPTIEKNVFIQNSAGQG
                     GAIYLYGNPVNPSDPVNPAVHIRPVIRRNTFISNTALISHGYAPPDNQYPANDHGDGG
                     AIVAFQGVDAEITDNRIGNNRADYYGGGIHCRQWSNSLIEGNTIESNSSLLGGGVHIT
                     YFSNPTVRSNTITQNNASSLGGGGIYVYYQSAPVIERNLIKQNSSTNGAGIAVFYQSS
                     PVIRNNLIVKNTDGSGIVMTSSWPQILFNTIADNDSTGIYLKSEGSSPAIENNIIASN
                     GSGWGIYMAPGNSPTVSYNNVWGNSKGQYYPDASDQTGRDGNISADPQFADSAGGNYH
                     LNITSPCINDADPAFSNPLGLTDYAGQNRILGSAADMGALEALPVWNLTSGSQYLVIQ
                     QAIDDSISGDRLAMIPGIYRGDGNRDIEFRGKSIVLQSLNPENPDIVNATIIDCQGSG
                     SSPHRAFWFRGQEDNDAVLQGITIINGGGRYDGGAIKCGGLIESQKNSNPTIRRCVFK
                     NNAATGYGGAIYVYSSNPVIADCVFISNMASDGYGGAIACTYASPLIANCIITGNKAV
                     GADHHGGGICCWGGNDAAGNLLPSNPLVINCSVTGNSADHRGGGFYAYWSQPTFINCT
                     VVGNRALEGGGIASFRESNPSVINCIVRDNRSPDGNQLALINTSRVWPASKPTAMTVS
                     YSNIKTGTGEITVDASCLLNWGSGNIDSEPLFLNPGMWIDPNTPANPHDDWFVSGNYH
                     IVPESPCLDAGDNLSVPWQSTADIDGEARICGSSVDIGADEVYMDPADFNSDGTVDTA
                     DLLLFADSWLQNDPMMDLNKDSIINAADFVRFASGWLWKAQWYPE"
     gene            11424..>14334
                     /locus_tag="PLE88_09055"
     CDS             11424..>14334
                     /locus_tag="PLE88_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOM76649.1"
                     /translation="MKHDRSRGWFEKGYIILALLLCLSPCGIAAGSNGFSHHPHSSSK
                     PLIGERYSNDRDANGVEDQLERRLEKTMRDAEKAVLPPDKEKIRRQLDEIIEVELIFK
                     EPIANTHLKKFELLGGQVDYIYKAVSYGWNGRIPLQKVRLLPEVMGSEFVLVQQTREI
                     EAHLDIATRNGRVRPVWAPGFAGSTSGFKGSDNITIAIVDSGIDADHPDMAGRCVFWH
                     DYTTDNSSVPVDKFHHGTHVAGIAAGTGVSAGSQTGTLYYTDRSITFLTYMPKGYFIP
                     SPISLPDGQVVFSSTASWYGGAPTSLYLFSKPKGVRLDSNVNNSLWTALSAPATGYSP
                     LQESNTFTASSDLIYTPALAANYVDAQHVVGRFAMVNSITNYPGSGDGFNRLSGIAPE
                     CRLAASKVFTNSGSGYTNWLESAIDDLVANRIEQNIKVINLSLGIIGDPGLSPSLRQK
                     INTAVANGILVVISAGNGGEGATAAERVVDDPGRAAYALTVAASNDENQLTDYSSQGF
                     LDPNTTPGQEEDYKPDITAPGGSIYYTYIMSVDSGSGDGGSFVDQQPNDYMQMRGTSM
                     ASPFAAGCAALVIDALEQNGKVWDFNSPADPFFVKMILCATASETNSPRENGLYSPTL
                     ERAAQGPGGFPAGKDLNEGYGLINADAAVEGLTCQYIVNSIESETLGSGPYDKRVWAR
                     RIFIPAGLMFEPTLLVPSGGDYDLYLYSFQPGLYGRPVIAASSTNVGQGFIESFIYQP
                     QTDFVGLLAVKRVSGAGEFILTPQKTLTLSSTNGGTIMDPGIGVFRYNKSDSVDVTAV
                     ADGGYHFVEWTGSAADAGKVQNPYSASTTITVDDNYTAIANFAVNIYTLTVTAGDHGS
                     VDPNGVLALAYGSSQLFTAAPDEGYTVDSWSIDGEIVQSGGLTYALENITADHVVLVT
                     FHPVNVTVSASAGDYGSVDPNGVLTLAYGSSQLFTAAPDEGYTVDSWTVDGTEVQS"
BASE COUNT         3578 a         3281 c         3991 g         3484 t
ORIGIN      
        1 gaaggcgctg ggatttcgta ttcccaagta tgcccatatg tctgtaaccg tcagcgatac
       61 cggcggcaag ctcagcaagc gcgaacgtcc gcaggctttg cgcaacgcaa tggcaggcat
      121 gaagcatctg gacaaggcag cagcggccaa ggccggcggc atttctcttg accagctgga
      181 aagcttttta aatggcgatt ccacaccgga tatgccggct attgatgcga tggcgcagta
      241 ccttggcgtt catctgccgg aaatcaatgt tgtcgacttc ttccgcagcg gctacctgcc
      301 ggaaacaatg gttaattttc tggccctgct gggctggaat ccgggtgacg ggcgggaaat
      361 aatgacccgc cggcagctga tagaatcttt tgacctgacg cgcgtgacca aggccaacag
      421 cttatttgac cgcagcaaac tgctgtcgtt taataccgag cacatcaaac tgacgccgat
      481 agatactctg gtcggccatt tccgcagcta cctcgaagcg gtgaagtccc cggtggcaaa
      541 ggccgacgat gcgatgctgg ctcggattat ccgtctcaat gaaggcgccc ggacgctggc
      601 gcaaatggag atgaaatcaa ggtttgcgtt catccccaac gaagaagttg tctatgaccc
      661 ggagtcggtc aaaaaggtgc tgctcaaagg cgacggcttg gcgattctgc aggaaatagg
      721 gcaggaactg ggggctctga cggagctgac ggcagagaat gtggaaacac tgctgaggga
      781 tctggcggcc cgccggcagg tcgggctggg caaggttgcg cagccgctgc gtgtggcgct
      841 gtgcggcaca accgtcagtc tgccgatttt tgatgcagtc cagatgctcg gcatcgccaa
      901 tacactcaag cggattaaac agacagtaag caagtttggt tctgctaagg aaggataaga
      961 ggtaatcgga tgtctctgct ggtaacgggt tcggttggaa ttgatacggt tcagacccct
     1021 tttggattga atcgggattg catcggagga tctgcggtct attttacgat ggccgccggc
     1081 ttctttacgg atgtcaagtt tattggtgtg gtcggcgacg attgcccttt taatctggag
     1141 gagctgttca gcggcaagaa agtggacttg acagggctgg aagttcgccg cggcagccgg
     1201 acattccgct ggcacggtac ctatgaaggc gatatgaatc aggcccggac agatgctgtt
     1261 gaactgaatg ttctggccga acgcccgccg gctgttccag acagttaccg ggattgcgaa
     1321 tatgtgtttt tggccaatac cgcgccattg cttcagatgg agctgctggc tcagcttgga
     1381 aatccggaat ttgtagcggc tgatacgatg aatctgtgga ttgagacggc caatcaggac
     1441 ctgcggcggc tgctggagca gattgacatg ctggtgctca atgaaaatga ggccaagctg
     1501 ctgaccggct atcacaattt aatcagcgcc gccaaggcta ttgctgcaat gggaccgcgg
     1561 attgtgatta tcaaaaaagg ccaatacggt tcaatgatgt atgaaaaaaa cggcagtatt
     1621 ttcctgctgc cggcctatcc gacggaagtg gtcattgacc ctacaggggc cggtgatgca
     1681 tttgccggcg gtttgatggg ctatctggct caagttcaga cggtcgattt gaccagtctt
     1741 cagaatgcga tggcctacgg gacagtaatg gcttcgttta ccatcggcga tttttccatc
     1801 tacggcattc gttcggccac gcaggaaaaa atcgagcagc ggtttgatat gcttcgcaag
     1861 gttacacagt tctgaggcag gtgaggaaac ggaatgcggc tgtttgcagc gatagatatt
     1921 gatccgcagg tgcgtgggcg gattgaagcg gttcaaaaac agctggcccg ccagctgaac
     1981 ctgaccggcc gggatgtcaa atgggtgcgg cccgaccaga tacatctgac gctgaaattt
     2041 cttggcgagg tgcgggatgc ggcggtaggg caggtttgcg atgcggttat acgaacggcc
     2101 ggccgcttca gcggcttcga ttttgaagtc cgcggcacgg gtgtattcgg ccggccggca
     2161 cggattgtgt gggcaggcgg ggcgccgtgt ccgcctctga tggaccttca ggcggcactg
     2221 gaagacgaat tcagccgcct cggatggcag aaggaaagcc gtccttttac ctgccatctg
     2281 actctgtgcc gcgttaaaag cgctgcggcc ggatacagac tggccgaggc aatcgaggct
     2341 tatcgggatg aaccgttcgg ttccgtgagc gtcagcagca tagtgctgta tcacagccag
     2401 ctggattcgg cagggcccca gtacagcgtt gtttgtacgg gggcactgaa atagtacaat
     2461 aaaacacaca gcgtgtcatt gaggatgaca ataatcaaac aggagtgttg aatatggcca
     2521 aagcaaagca gaaaaccgcc gacaaagaaa cgaaaactgt cgattccgcc agaagcgatg
     2581 ccctcgaccg tgcgattgcc cagattgaaa agcagtatgg tgtcggctct attatgaaaa
     2641 tggatgaatc ccagcatccg aaaatagaag gtatttccac cggcgctctg tcgctggata
     2701 ttgccttggg aggtgccggc atcccgcgcg gccgtattgt agaattttac ggccctgaat
     2761 cgtccggcaa aacgaccttg gctcttcacg tgatagccaa tgcccagaaa gccggcggtg
     2821 tggcggcctt tattgatgcc gagcatgcat tggacacaac atgggccaaa aaactcggtg
     2881 tagatatcag cggtctgctt atcagccagc cggataccgg cgagcaggcc ctcgacatcg
     2941 ccgagatgct ggtaaagtcc aatgcggtcg atatcatcgt gattgattcg gtggcggcat
     3001 tggttccggc agccgaattg cagggcgaaa tgggccaaag ccatgtcggt ctgcaggcgc
     3061 ggctgatgag ccaggcactg cggaaattga ccggtgttat cgccaaaagc aaatgctgtc
     3121 tgattttcat taaccaaatc cgtatgaaga ttggagtgat gttcggaaat cccgaaacta
     3181 caaccggcgg caatgccctg aagttttatg catcggtgcg ggtggatgtg cggcggatat
     3241 ccaccatcaa agctgccaat gatgaagcga ttggcaaccg tgttcgtgcc cgtgtggtta
     3301 aaaacaaggt tgctccgcct ttccgggctg ctgaattcga tattatgttt gacagcggca
     3361 tcaattatgt tggcgatctg ctggatttgg ctgttgaatg cggccttgtc aacaagagcg
     3421 gggcgtggtt tacctgcggc gaccttaagc tggggcaggg acgggataag gcaatgcagt
     3481 atctcaaaga caatcctgcg ctgctttgtg aactgaagga taaggtgctg gccaaccgcg
     3541 gtctgattca gtgcagggac agcgggaagg aagcgtccga cgagaccagc gaataaccaa
     3601 caggaataat cagtcaaaac aaatgcggca gatgccgcgc taaacttttt ttcggatagg
     3661 tccatggtgt taagttcaaa agacattcgc agcggattta tagaattttt caagggacac
     3721 aaccacttgt ttattcccag ctggccggtg gtgccgattg gcgacgaaac actgctgttt
     3781 accaatgccg gcatgaacca gttcaaggaa tactttctgg gccatcgaac cccctcattc
     3841 aagcgggccg tcaacagcca gaagtgcatt cgtgtttccg gcaagcacaa cgacctcgag
     3901 gaagtcgggc tggataccta tcaccataca ttcttcgaga tgctgggcaa ctggtcgttt
     3961 gatgattatt tcaaggccga atcgattgaa tgggcttggc agctgctgac gggggtgtat
     4021 ggcattgccc cggaccggct gtgggcgact gtgtttgccg gcgataaaga ggataatgct
     4081 gctgcggata ccgaagccgc tgaactctgg accaagctga cgcctctgcc caaggagcgt
     4141 gttttgttct gcggacgcaa agacaatttc tgggaaatgg gcaataccgg tccctgcggt
     4201 ccctgcagcg agatacatat tgatttaggc cctgaccgct gcgatatgaa gcatattccc
     4261 ggccatcgat gcgccgtcaa tgctggatgc agccggttta ttgaattatg gaatctggtt
     4321 ttcatccaat tcaaccgcaa gcctgacggc aggcttgagc gccttagtgc caattatgtc
     4381 gataccggcg caggtctgga acgcattacg gctgttttac agaacaaacg cagcaactat
     4441 gacacggatt tgtttttgcc gataattcaa tcggttgagc aaattgccgg ccgacactat
     4501 acatcgcagc tgggcaataa aaccgacaac gcctttcggg ttatcgcaga ccatatccgc
     4561 acgcttacat ttgcaattgc tgacggcgtc aatcccagca atgaaggacg cggctatgta
     4621 atgcgccggc tgctgcggag ggcctgccgc ttcgggcggt cgctgaatct tcacgagccg
     4681 tttatgtata aactggtgga tgtggtggta gataatatgg ggcaggcctt tccggaaatc
     4741 cgcgagcggc gggagtttgt ggcatcggtc attcaggccg aagaggcctc gtttggacgc
     4801 acgctggacc gcggtctgga aattttcgcc aacgccgcaa aagatgcaaa aaacgggatt
     4861 atctctggtc aggatgcttt ccagttatac gatacctatg gctttccgct ggacttaacc
     4921 cagctgatgg cgcgggaaca gggacttaca gtggatatcg atgagtttga acggctgatg
     4981 acccagcagc gtcagagagc acgggctgcc cagaagaccg gctctctggc ggcaacttta
     5041 accggcgtag aactgccgga aaccgatgat tcgctcaaat atgaaacgga aacctgcaga
     5101 gccaagctgc tgggctggat aaatgacgac ggctttacca cgaagggaga gttaacggat
     5161 acagaacatc cggcagccct tgttttggat aaaacctgct tctatgccga atcgggcggg
     5221 caggttggcg attgcggaat aattaaaact gaggatgcgg aattctgcgt tgaaacaaca
     5281 gataagattg ccgattgcgt gctccatcgc ggaagactcc tgtccggttc gctgtgggtg
     5341 ggggatcagg ttcaggcgtg tgtggataaa agccggctgg ccagccgcaa gaaccatacg
     5401 gcaacgcaca tcctgcaatg ggcactccag acagtactgg gccaaacggt taagcagcag
     5461 ggatcgctgg tttgtcctga ctatctgcgg tttgatttta cgtggcccaa ggcactgacc
     5521 aaagaacaga ttcagcaggt cgaagcgctg gtgcaggaca agatagaagc cgcactgccg
     5581 gtcacaacag ccgttatgga tatcgaccgg gcccgtcagc ttggtgcaat ggctcttttc
     5641 ggcgaaaaat acggccagca ggtgcgtgtc ttggcaatcg gcgcctctga taaggaccat
     5701 atcaaggatg ccttcagcaa agaattctgc ggcggaaccc atgtatccaa tacggctgaa
     5761 atcggcggtt ttgccattca aaaagaagaa agcatttcgg ccggcgtacg ccgtattacc
     5821 gcgttgacgg ggccgggtct gatgcgttat ctgctggacc gcagccggat tgcagatgag
     5881 cttgtcgaat tgctcaaagt ccctgccgac cagattgttc agcgcattgg gaaactcgtg
     5941 gatgagaaca aaacgctcaa aaaggaactt aaatccggcg tcggaaaatc agcatcggat
     6001 gcctttggcg ccgccgataa gctgctcgaa tctgctcaaa aagcggggga agcttttgtt
     6061 gtagtggggc aattgccttc ggtggatgtg gaacaggccc gagcggctgt tgacagcatt
     6121 aagaaaaaag cccgctgcgc tgcaattgta tttggcatgg taaccggcga caacaaggta
     6181 atgcttctgg caggtgttac ggaagatctt attcaaaagg gactaaaagc cggtgatatt
     6241 gtcaaggaaa tcgccccgat tgtcggcggc ggcggcggcg gaaagcctaa tatggctcaa
     6301 gccgggggca aagaaccaga caaacttgac caagctctgg aacgggcaaa acagattata
     6361 acagcacatc tgggctgatt tgttaaacaa gcagtgcata ttggattgga aaagtctcta
     6421 tcgtggtctt ttttgacgat taatctgtta ctaattcgat tggccggcta tgaattggaa
     6481 gatatttcaa aatcagcaga accgactttt gcggaaaata aaaaaggaag aaaatataaa
     6541 aaaaatacag tttgtttttg attttaagtt ctttaattga aataggatat aaacaagata
     6601 gatggtatcc attgaaattc tgtaaaaatg tggttgacac ggatagacca gatagggtaa
     6661 attgtcggat gaattagtgt gtgcggagaa gagatggaaa gagaaggatg cgcctatctg
     6721 accagctcct ctaaaaaagg atggaataga gtatgtttat aaggatcaga aaggagagga
     6781 aagatgaaga agtttgcggt actgatgtta ttaatttcag tggtgactgc acaggcgagt
     6841 ttgaaaattt atgtgaaatg ggatgaggga ccgtacggga gtatgtacat tgattacccg
     6901 gacagcaagt taaccattaa ccccagcgat gtgattacta tagggataat ggatatcctc
     6961 gggcaaacac aaccgggtgc tttggctttg ggaatttcaa tgggacccgg cagtctcgat
     7021 gccagcggaa tgaagccctt ggcgggtgta tcggcagcat tgacaaatga tccggatagc
     7081 gcggcccagt atggtttgct cagtccatat gtagcccttg aaattgggcc caacatgacc
     7141 ggaatgctga ttagagacct tctgttccat tgcgatggac cgggcgatgt aacaatcgtg
     7201 ttggtcgatg agaacgggat gattgtagac agccaagtaa tccatcagcc cgaacccatg
     7261 acccttggcc tgttgggctt aggcggactg tcgcttgtga tgtctcggaa gcgccgggca
     7321 gcttaataaa cataagctcg ctgagagtgg tttttgtgca gttattccag attgttggat
     7381 aagaaatctg gaaagagtag gagtttttag aattatttcg agtgctgaag tgtggtagtt
     7441 gaagtgtgtt gggagtatgg tgaactgaga tgattttttt tgggaggtag gcgttatgat
     7501 gaaaagaata ctaatgatgt tgcttctttg cggcttggcg aatgcgtcgt tgtatatcac
     7561 agtcaatggt gtaatcaatc cccccgatag tactgttact attaaaccga gtgataccct
     7621 gtggataggt atttgggatg atggtcagac agctcccggc ttgcttgcaa tggggcttac
     7681 aatggggccc ggcagtttag acgcaagcaa gatgtcggtt caggccggtg ttttggctgc
     7741 ggtatcagat gatgcaggca aggccggcga tctgggtctt caaagtccat ttatcgcaat
     7801 ggagataggg cagatccagc cgggcactct tatcagtgaa gtgctgttcc actgtgatgg
     7861 cccgggcgat gtaacaatcg cattagtgga tgacaacgga atcgtcgttg acacacaagt
     7921 tatccatcag gttcccgaac cgtttacatt ggcaacgctg gcgcttggca gcttactgct
     7981 gagacgcagg attgcctaat acacgacgcg gtttttattg aaaaattagt ggttgatata
     8041 cagggacttc ccttaaaagg ggagtccctt tttattttgg ttttaattta cagaaaacat
     8101 tgttctcgat aaacatagaa gaaacggaag gattataaga ctttgccgca ggatttgttt
     8161 tttgaaagaa tagcggtgcc cgggatatat tcctgacttt aatttcccat ccattatctt
     8221 tgacttagtt tgttttttct gctataataa cgcaaattag aatgaggttt attttgtttt
     8281 gctgacaggg aaaagaattg tcattttgag gagaccagga acaccgcggt tgtgagatat
     8341 tgtttctgtt ttagggcatc ttaaaaaatt aattatatcg gcaggcgggt tatgaaaagg
     8401 gagctttact tcttactgtc tgttcttttt tcagcaagat tagtcggggg gactttgtat
     8461 gtcccgaccc aatatggttc tatccagtcc gctatcaatg ccgccgcaga aaaagatgtg
     8521 attattgttc agccggggac gtatgtagag aacatcaatt ttttggggaa aaaaataact
     8581 gttcaatctg ctgatccgga cgatcccaat gttgttgccg ctacgattat tgacggttca
     8641 gccccagatg atccagcaca ggcatctgtt gttaccttca gcagcggcga agataatgaa
     8701 tcgattctga cgggttttac gattcgggga gggaccggtt catggattgc ggttgcgtgg
     8761 aaatatcagg gcctgcggtg gaatcgatgc ggcgggggtg tggtatgcta taatctgtca
     8821 gcccccacta ttgaaaagaa cgtatttatc caaaattctg ccggtcaagg cggagccatt
     8881 tatctgtatg gcaatccagt caatccctct gatccggtga atccggcggt tcatattcgc
     8941 ccggtcattc ggcgcaatac ctttatcagc aataccgcac tgatcagcca tggatatgcg
     9001 ccgccggaca atcagtatcc ggctaatgac catggcgatg gaggggcgat tgtcgcattt
     9061 cagggcgtcg atgctgagat tacagataat cggatcggga ataaccgagc cgactattac
     9121 ggcggcggca tccattgccg ccagtggagc aatagtctga tcgagggaaa tacgattgaa
     9181 tcgaatagct ccttgctggg cggcggagtg catatcacct atttttccaa cccgaccgtc
     9241 aggtcaaata ccatcacgca gaataatgca tccagtctgg gcggcggcgg tatatatgtc
     9301 tattaccagt cagcccccgt tattgaaaga aatctcataa agcagaattc ctcgactaat
     9361 ggagccggga tagcggtttt ctatcagagt tcacccgtta ttaggaataa tttgattgta
     9421 aaaaataccg atggttccgg tattgttatg accagcagct ggcctcagat tttatttaat
     9481 actattgccg ataacgacag tacgggtatt tatctaaaaa gtgaaggaag cagtccggcc
     9541 atagaaaaca atattattgc gtccaacggc agcggctggg ggatttatat ggccccgggc
     9601 aattccccaa ctgtttctta taacaatgtc tggggcaaca gcaagggcca atactatccg
     9661 gatgcatcgg atcaaaccgg ccgggatggg aatatctctg ccgatccgca gtttgcagat
     9721 tctgccgggg gtaattacca tctgaatata accagcccat gcattaatga tgctgatccg
     9781 gcattttcga atccgctggg cttgaccgat tatgccgggc agaaccgcat tctcgggtcc
     9841 gccgcggata tgggagcatt ggaagccctg ccggtttgga atctaacaag cggcagtcaa
     9901 taccttgtta ttcagcaggc aatcgatgat tccatcagcg gcgatcggct ggcgatgatt
     9961 cccggaattt accgaggaga cggcaatcgc gatatcgaat tcagaggcaa atcgattgtt
    10021 ctgcagagct taaatcccga aaatccggat attgtgaatg ccaccattat tgactgtcag
    10081 ggttcaggca gcagtccgca tcgggccttc tggtttcgcg ggcaagaaga caatgatgct
    10141 gttttgcagg gcattacgat tataaacggc ggcggcaggt atgatggcgg ggccattaaa
    10201 tgcggcggtc ttattgaatc tcaaaaaaac agcaatccta caatccgccg atgcgtattt
    10261 aagaataatg cggccactgg ttacggcggt gcgatttatg tttacagttc caatcctgtc
    10321 attgccgact gtgtatttat cagcaatatg gccagcgatg ggtatggcgg cgctatcgct
    10381 tgtacctatg cctcgccgct tattgccaat tgtattatta ccggcaataa agccgtcggt
    10441 gccgatcatc atggaggggg catctgctgc tggggcggaa atgatgctgc cggcaatctc
    10501 cttcccagca acccgcttgt catcaattgt agtgtgacgg gtaattcagc agatcacagg
    10561 ggcgggggat tctatgccta ctggtcccag ccgacattta taaactgcac cgtcgtcgga
    10621 aatcgggcgc tggaaggcgg aggaatagcc tcttttcggg aaagcaatcc ttcggttata
    10681 aactgcatcg tccgggacaa tcgctctccc gacggcaatc agctggcact gattaacaca
    10741 tcgcgcgtct ggccggcaag caaaccgaca gcaatgaccg tttcctacag caacatcaaa
    10801 accggcaccg gcgaaataac agtcgatgca tcctgtctgc tcaattgggg cagcggcaat
    10861 atagacagtg aacctctgtt tcttaatccc ggaatgtgga ttgacccgaa tacgcctgca
    10921 aatccgcacg atgactggtt cgtcagcgga aactatcata ttgtgcctga atccccatgt
    10981 cttgatgccg gcgataatct ttctgttccc tggcagtcaa cggcggacat tgacggagaa
    11041 gctcgcatat gcggcagcag cgtcgatata ggagcggatg aagtgtatat ggatccggcc
    11101 gattttaata gcgacggcac tgtggatacg gcggatttgc ttctgtttgc cgattcgtgg
    11161 cttcagaatg atccaatgat ggatctgaac aaggatagta ttataaatgc agcggatttt
    11221 gtccgttttg cttctggatg gctatggaaa gctcaatggt atcctgaatg aaggttggaa
    11281 aaaacgatca ggatgtcaag gccgatcggt accatattga ccagcagggt caagaaggtt
    11341 ttgaaacatg acatagaaag aggggtaaaa cagcaggcag tacgctgtct tttgggcagt
    11401 ttttaataaa tatggggcat tgtgtgaaac acgatcggag cagagggtgg tttgagaaag
    11461 ggtatattat ccttgcgctt ctattgtgtc tatctccctg cggaattgcc gccggcagca
    11521 atgggttctc ccatcacccg cattcctcct ccaaaccgct gataggagaa agatattcga
    11581 atgaccgcga tgccaatggc gttgaagacc agttggaaag acgtcttgaa aagaccatgc
    11641 gggatgcaga aaaggcggtt ttgccgccag acaaagagaa aatacgcagg cagctggatg
    11701 agataataga agtcgagctg atatttaaag aacctattgc caatactcac ctcaagaaat
    11761 ttgagcttct gggggggcag gtagattaca tctataaagc cgtatcctat ggctggaacg
    11821 ggagaatccc tctgcaaaag gtccgtctgc ttccggaggt tatgggttcg gaatttgttt
    11881 tggtccagca aacccgtgag attgaagccc accttgatat agcaacacgc aacggacgtg
    11941 tccggccggt ctgggcgccc ggttttgcag gcagcacaag cggttttaag ggctcggaca
    12001 atattaccat tgctattgtt gattcaggca ttgatgcaga tcatccggat atggccggac
    12061 ggtgtgtgtt ttggcacgac tatacaacgg ataattcatc ggttcctgtt gataaatttc
    12121 accatggcac ccacgtagct ggtattgcag ccggcacggg ggtttcggca ggttcgcaga
    12181 ccggtacgct ttactataca gatcggagca tcaccttcct cacatatatg ccaaaaggct
    12241 actttatccc cagccccatc agtctgcccg atggacaggt ggtcttttcc tctactgcaa
    12301 gctggtacgg cggcgctccg acatcgcttt acctgttttc taaacccaag ggagtaagac
    12361 tggattcgaa tgttaataac agcttgtgga cagcactatc agcgcctgca accggctact
    12421 cacctttgca ggagagcaac acatttacgg catccagcga tttgatttat acaccggctt
    12481 tggctgccaa ttatgttgac gcccagcatg tagtggggcg ttttgcgatg gttaattcca
    12541 taacaaatta ccccggcagc ggtgatggat ttaacagatt atcaggcatt gctcccgaat
    12601 gcagattggc ggcatcgaag gtgtttacca acagcggttc cggttatacc aattggctgg
    12661 aatcggcaat agatgacctt gtagccaatc gcatcgaaca aaatataaag gtgataaact
    12721 taagtctggg catcatcggt gaccccggcc tttctcccag tttgcggcag aaaatcaata
    12781 cggctgttgc caacggcatt cttgttgtta tttcggcagg caacggcggc gagggggcta
    12841 cggcagcgga acgtgttgtc gatgaccccg gcagagcggc ttatgccttg acagttgccg
    12901 cttccaacga tgaaaatcaa ttaaccgatt attccagtca aggcttttta gatcccaata
    12961 caacaccggg acaggaggaa gattataagc cggatattac agctccgggc ggttctattt
    13021 attataccta catcatgtct gttgacagcg gttccggcga cggcgggagt tttgttgacc
    13081 agcagccgaa tgattacatg caaatgcgcg gcacatcaat ggccagcccc tttgctgccg
    13141 gctgcgcggc tttggtgatt gatgcacttg agcagaatgg gaaggtttgg gattttaact
    13201 ccccggcgga cccgtttttt gtcaagatga ttctgtgtgc gacagcttcg gaaaccaata
    13261 gcccgcggga aaatggtctt tattctccaa ccttggaaag ggcagcgcag ggaccgggcg
    13321 gatttcctgc aggcaaagac ctcaacgagg gctatggcct gatcaatgcc gatgctgctg
    13381 tcgagggttt aacctgccaa tatatcgtga attcaataga gtccgaaaca ttgggcagcg
    13441 gtccttatga caagcgtgtg tgggcacgcc gtatttttat tcctgccggc ctgatgtttg
    13501 aacctacttt acttgtgccc agcggcggcg attacgattt atatctttac agttttcagc
    13561 ccggcctgta cggcagaccg gttattgccg catccagcac caatgtcggc cagggattca
    13621 tagaatcatt catttaccag ccccagaccg actttgttgg gctgttagcg gtaaaaagag
    13681 tatcaggggc gggtgaattt attctaacac cccaaaagac tcttactctg tcttcaacaa
    13741 atggcggaac aataatggat cccggcatcg gtgtatttag atacaacaag agcgattcag
    13801 tcgatgttac tgcggttgca gatgggggat accattttgt tgagtggaca ggcagcgctg
    13861 cagatgcggg caaggtgcag aatccctatt cagccagtac aactatcaca gtcgatgaca
    13921 actatacagc catagccaat tttgcggtaa atatatatac acttactgtt actgccggcg
    13981 atcacggttc tgtcgacccc aacggggtat tggcgcttgc ttacggttcc agtcaattgt
    14041 ttacagcggc acccgatgaa gggtacacag tcgattcctg gagcatagac ggcgaaatcg
    14101 ttcaatccgg cggcctgacg tatgcgctgg aaaacatcac agccgaccat gtggttttag
    14161 taacattcca tcctgtaaat gtgacggtgt ccgcttccgc cggcgattac ggttctgtcg
    14221 accccaatgg ggtattgacg cttgcttacg gttccagtca attgtttacg gcagcaccgg
    14281 atgaaggcta cacagtcgat tcttggacag tagacgggac agaggttcaa tccg
//
LOCUS       DAOTBO010000075        13508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3846_length_13508_cov_4.796997, whole genome shotgun sequence.
ACCESSION   DAOTBO010000075 DAOTBO010000000
VERSION     DAOTBO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13508
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_13508_cov_4.796997"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..2790
                     /locus_tag="PLE88_09060"
     CDS             172..2790
                     /locus_tag="PLE88_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76650.1"
                     /translation="MKKMMFFLTLLSVIGSSAMAATVAYWRFEQGPAYTNVTHNAAAG
                     VYSPDIPDVSGNGNHLAVWSSSQWQYRANVPYDTVPWTGAPNNFSVKNITGTPTMWTT
                     TLQNWEPAQWTIEVTFKAEAGGWKTLVGRDSQGALTQGTELNPALAGLYLQTTDNPPM
                     GLAIKFIDRAGYWHSAELGNAYIGFNYGSDPDGNTAPWYTIAATSDGRYLRLYRFSHA
                     TPEKGYELIAETDMTVVNPGSTDTALDGGAGGGSDWQAGNITVGRGMYNGVHTDRFYG
                     YIDEVRISDRALRPEEFLLSEPFVKPVYPAQDEYLMSTGAIAYSWQNNIPEGKTFGSY
                     TIYIADNPADLATSVPAAYLHKGTVTDEQDTTYEGYAGTYAYGQDYYWRLDLTLLEPN
                     YLPDPNNPVYNIPIVYEGWPVRFFGPRACPGLTISGNVAILPDPIDHSYAPTDAVFTV
                     TINRGTINNSAIAWYKVNGAQDDLTSPDPLDPADIQISNVPGVTEITYNPDLPTATQT
                     TLRIVAADPADNGRYYARVRLADPLGGPTGCEANSPAAELFVRDDTYAATNYLVHRYG
                     FAGDASDSIGGAHGTIADVNEPNHSFADGQLILSNPGLNSRPTRPSTDPLYPNLQELL
                     PQQGAYVDLPNGMISALGKTATFMIWFTYKSADTGDWPRLFDFGTSTGGEGFSTGGDG
                     VQWLNGVPYLDTAAEDTQEYIMLCPKRGSGPGWRYESLVRPPDNGVIIDPADNSAIAG
                     GNEACIACVFDGVNRVMTLYVNGVQVGQNTNLTHDLANINDVNNWLGRSQWPDAMFTG
                     KINEFRIYNIPLTKHWIKAYYEQGPDDYTTVPNPCIQDAPNPMDFNADCFVDFSDFVV
                     FAAQWLDCDRLNGCNQ"
     gene            2984..4378
                     /locus_tag="PLE88_09065"
     CDS             2984..4378
                     /locus_tag="PLE88_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76651.1"
                     /translation="MKNRSVLLSLLFLISVIWPTSECAAWGAVVAYWRFEEGPPGDGV
                     PHGASVGVWSPDILDYSGNGNHLSVWSTGGYAGYAWTSDVPKPAIPTTGQSNTLSVKN
                     TGAYPGMWTQTGSGISTMSPSAFTIEASFKLENGDYRTIVGRDSYGTASVNANLAALY
                     FQALPNNALAIKFCDVSGFWHQAVSAEGIFQSFDYSTNPSGEGVPWYSMAAVSDGSTL
                     SLYLLEHGRDTGYQLIARTDMTLSGSPSTVLTAGAGDGSDWDAGNWSVGRGLYNRAHT
                     DRAYGFIDEVRICNRALQPSEFLFYEHVPQGLVISPQNLTLSEQDAGQGDLFFSLEYP
                     PAGTVVLEISELYGRGQIILDKTMLTFTPADWNIPQAVRITAVDDADVENAEHPVLLS
                     VVVSSSQDPLYDGLEVEPVRITVADNECGAWGYAPGDYNRDCIVNLYDFVTFARYWMD
                     CSDPERIGCTNFYP"
     gene            4426..6813
                     /locus_tag="PLE88_09070"
     CDS             4426..6813
                     /locus_tag="PLE88_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM76652.1"
                     /translation="MNKMNQGRGVLLIVLALGFAGPLFAWTLQQAPVMTQWAYQVNPA
                     EPLPEYPRPQMVRPEWMNLNGIWQFQPGTAGDTVPVGQVLSQDILVPFPVESAISGIM
                     QHYDRLWYRRLFEIPSRWNGQRILLHFGAVDWETEVYINGSSVGIHKGGYDAFSFDIT
                     PFLLPAGQQELIVRVYDPTDNGGYPRGKQTLSPGGIMYTPTTGIWQTVWLEPVPQISL
                     TELKLIPDVDHKLLNITGWVSQPAANLTIQATAYDNGVPAGTVSGAVGDQLQLVMHNP
                     KLWSPETPFLYDLKVVLKQGSTVIDQVDSYFGMRKIALGTVDGVVKMLLNDTFVFQMG
                     PLDQGFWPDGIYTAPTDAALRWDLEQTKALGFNMTRKHIKVEPARWYYWADKLGLLVW
                     QDMPSVNSYTSNPQPIDKAQFKTELTQMVRQHWNSPAVIMWVIFNESQGQHDTAPLCA
                     MVKAMDPSRLVNQASGGSHYGAGDVLDIHNYPPPSYPVSSTQARVCGEYGGIGMRISG
                     HMWNPNSWGYTMVNSAEELASVYDSYSQQLISFKQNQGLSAAVYTQITDVEIEINGLI
                     TYDRAVVKADAGQIRLSNLMYARTYQDVLPTSQQTPQLWRYTTSTPPSNWSAPSFHDA
                     SWTEGPGGFGTAGTPGAVIGTVWNTSDIWLRRMFNPGPLTPADLDRLVWRIHHDEDAE
                     VYINGALALATTGYTTSYIPLPAAAEAKAALVPNADNVIAVHCRQTIGGQFIDVGLAL
                     ETIHASSGACGQWGYAPADLNYDCAVDIEDLALFIADWWTCSLPGRLECINYLLP"
     gene            6866..9505
                     /locus_tag="PLE88_09075"
     CDS             6866..9505
                     /locus_tag="PLE88_09075"
                     /inference="COORDINATES: protein motif:HMM:NF028477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HOM76653.1"
                     /translation="MVKNGKHVETMEIALRIIKPGRQQLIGLLMLCLLPLPAQSAWMP
                     AVRPPAVPLVACDPYFSIWSCADELTADTTRHWTGKPHPLTSLIRIDGRTYRLMGNKP
                     EGLPALRQTSVTVLPTRTVYAFEGEGLSVIMTFLQPALPDDIDLLSRPITCLTWQCAS
                     ADGKAHEVAVYFDAGMELVVNDPSQAVVWFEENSPDMVVLKAGSKDQNILGRKGDDVR
                     IDWGYFYLAAAKSDHPVGAIASPDVCRQSFAQQGRLTQAIDSQLPREVRDNSPVMAMQ
                     FDLGRIAAVPKTCRVILSYDDMQSILYFGRRLEAFWKRDGKQIAQIIKETAENYSALA
                     RRCEQFDQELLKDLEQTGGPDYQIIGALAYRQALGANKIVADKNKMPLMFSKECFSNG
                     CIGTVDVFYPFAPQVLLLNPTLAKASFVPILEYGRSQRWKFPFAPHDIGTYPHAMGQV
                     YGGGERSEENQMPVEECGNMILLVAAVAEAEDDIAFAKQYWDVLTTWAEYLKSKGLDP
                     ENQLCTDDFAGHLAHNVNLSVKAVVALGAYAKLAEKMGDPQTAAAYRSTAEQYVQDWM
                     RMADDGDHYRLAFDRSGTWSQKYNLVWDRILGLNLFPPAVAQKEMEFYKRIQKPYGLP
                     LDNRSDYTKLDWIVWTASITGRQDDFEALIAPVVKFINETPDRIPLTDWYWTHNAKHR
                     GFQARPVVGGVFIRLMDNRQIWEKWVSRADSIRGTWAPLPKPPQIIDVVPVSLENGYR
                     WRYTFSQPDDNWYKPEFDAASAGWKEGAAGFGTPQTPSTEVRTRWNTSDIWIRRQFTL
                     EQDALDNLCLMIHHDEDAQIYINGTLAAVLTGYTTGYQPYPLTDQARSALKTGLNTIA
                     IHCRQTGGGQYIDAGFVKVIPQE"
     gene            9673..11877
                     /locus_tag="PLE88_09080"
     CDS             9673..11877
                     /locus_tag="PLE88_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOM76654.1"
                     /translation="MEAKTQVLFLLLSCSLFAAAWQPAQAPLMTQWAEDVTPNNVWPE
                     YPRPHMVRQNWQNLNGLWDYAVVSQDAPKPDSWDGKILVPFCIESALSGVMKKVQPSQ
                     SLWYRRTFQSSLDKSSKRLLLHFGAVDWQAAVWVNGKQVGRHTGGYDPFTFDITDAVV
                     DGQNELVVRVWDPTDTGSQPKGKQVLRPGGIMYTAVTGIWQTVWLETVPSLYIQSLKI
                     VPDVDRSVVQITVSASNTAEAAITVKDNGRTAAQAQGKTGQPIEVKLEACKLWSPDSP
                     HLYDLEISLMDASGAADRVSSYFGMRKIEVKKDENGINRLFLNNKVLFQYGPLDQGWW
                     PDGLYTPASQAAMEYDIVMTKKFGMNMARKHVKYECARWYYLCDKLGLLVWQDMPSGD
                     TGRDEESKSNFRTELKAMVDALHNLPCIVMWIPFNEGWGQHDTEEIGQWMQNYDPTRP
                     INEASGWHDRGSGDVSDMHNYPGPGVRPAEDKRVCVLGEFGGLGLPIEGHLWQPDRNW
                     GYVSYKNTEELTDAYVGLLTAMRPLIAQGLSAAVYTQTSDVEIEINGLMTYDRKVVKM
                     DLGRCAEAAAKLYRRPPAVKTLVETSQQQRQTWRYTTQSPSNNWVETAFDDTSWRTGR
                     GGFGTSGTPGAVVGTVWNTSDIWMRRTFELDTVPAQGDLLLVIHHDEDAQVYLNGKQI
                     KTLSGYITNYTFAPLSPDALKLLKTGVNTLAVHCRQTSGGQYIDVGLSLLLD"
     gene            11967..>13508
                     /locus_tag="PLE88_09085"
     CDS             11967..>13508
                     /locus_tag="PLE88_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM76655.1"
                     /translation="MKQWALLLGIVGTALAAEQITVVDRPDVHSVNSFYVSNRPPLEP
                     SRLIELPVGSIKPAGWLLTCLRRQRDGLTGNLGNISAWLQKEDNAWLSLDGKGRWGWE
                     EVPYWLRGYGELAYILDDPQMIAETKIWIEGALRSQRDDGDFGPDQRFNDGTRDYWAN
                     MLMLYCLQSYYDYSADQRVLDLMRRYFRHQSSVPDERLLTGYWQRMRGGDNLYSIYWL
                     YNRTGEKWLLETAEKIHRNTADWRMEGDLPNWHNVNIAQAFDEPAVWFLQSRNPADLE
                     FAYKNFHEVRRRFGQVPGGMFGGDENCRAGYDDPRQCIETCGMIEQMLSDEMLVRISG
                     DPFWADHCEEVAFNSYPAALMPDFRSLRYLTAPNMAASDRHNHRPGIDNGGPFLLMNP
                     FSSRCCQHNHSHGWPYYVKNLWLATPDNGLCASLYAANEVRAKVGDGTEITLRQETHY
                     PFDQQIRLTLLSEKPVRFPLYLRVPGWCENPAVQINGMPRSIAAQPRQYIRLERVWTG
                     GDTVVL"
BASE COUNT         3148 a         3408 c         3766 g         3186 t
ORIGIN      
        1 accagttcct gatggttgtg ccggaaccgg caacgctgct gctgctgggt atggggtcgc
       61 ttcttgcgat tcgcagaaag cagtaattga tgagtaaaaa gggaaccgga gtaggatatg
      121 tgtcctactc cgatgctccc tgctacacat tggtttttca ggagacagac tatgaaaaaa
      181 atgatgtttt ttctaacact cttgagtgtt ataggttcct ccgcaatggc ggcgacggtg
      241 gcttactggc ggtttgagca aggccccgcg tacacgaatg tcacccacaa tgcagccgcc
      301 ggtgtttaca gcccagacat tcccgatgta tccggtaacg gcaatcattt ggcagtctgg
      361 agcagcagcc aatggcagta tcgcgcgaat gttccttatg atactgtccc ttggactggt
      421 gcccccaaca atttcagcgt caagaacatc acgggtactc ctactatgtg gacaacgacg
      481 ctgcagaact gggagccggc tcagtggact atcgaagtga ccttcaaggc ggaagcaggc
      541 ggctggaaaa cgcttgtcgg ccgcgattcg cagggagcac tgacgcaggg tacggaactc
      601 aatccggcgt tggcgggttt gtatctacag acgaccgaca atcccccgat gggccttgcc
      661 attaagttta ttgacagagc tggatactgg cattcagccg aactcggcaa tgcctatatc
      721 ggctttaatt atggaagcga tccggacggc aacactgctc cgtggtacac aattgcggca
      781 accagcgatg gccggtatct gcgattgtac cgcttcagtc atgctacgcc tgagaaaggg
      841 tatgaattga ttgcggaaac ggatatgacc gtcgttaacc cgggcagtac tgacacggct
      901 ctggatggcg gagccggcgg cggcagcgac tggcaagcag gcaatattac ggtcggacgc
      961 ggcatgtaca acggtgttca cacagaccgt ttctatgggt atatcgacga agtgcggatt
     1021 tctgacaggg ctttgagacc tgaagaattc ctgctgtcag aaccttttgt taaaccggta
     1081 tatcctgctc aggacgaata tctgatgtca accggtgcca tagcgtattc gtggcagaac
     1141 aatattccgg aaggaaagac attcggctcc tacaccatct atattgctga caatcccgct
     1201 gatctggcca cgtctgtgcc cgcggcctat ctgcataaag ggacggttac tgatgagcaa
     1261 gacacaacct atgaaggtta tgccggcacg tatgcctatg gccaagatta ttactggcgt
     1321 cttgacctga cgctactgga gcccaattat ctgccggatc cgaacaatcc ggtatataac
     1381 attcctattg tgtatgaagg atggcctgtt cggttcttcg gcccgagggc ttgtccggga
     1441 ttgaccatca gcggcaatgt ggcaattctg ccggacccga ttgaccacag ctatgccccg
     1501 acggatgccg tttttaccgt aacaattaac cgcggtacaa tcaataattc ggcgattgct
     1561 tggtacaaag tcaacggcgc acaggacgat ctgacttcac ccgatccgct ggacccggct
     1621 gatatccaga tttcgaatgt tccgggcgta accgaaatta cttataatcc tgatttgccg
     1681 accgctacgc agacaacgct gcggattgtt gcggcagatc cggctgacaa cggccggtat
     1741 tatgcccgtg tacgtctggc ggatccgctg ggcggaccga ccggctgtga agccaattcg
     1801 ccggcggcag aactgtttgt ccgtgacgac acctatgctg cgaccaatta tctggtccat
     1861 cgctacggat ttgccggcga tgcttccgat tctatcggtg gggcgcatgg aacaattgcg
     1921 gatgtcaatg agccgaatca ttcgtttgca gacggccaat taatccttag caatccggga
     1981 ctcaacagca gacccacccg tccgtcaacc gatccgctct atccgaacct gcaggaactt
     2041 ctgccgcagc agggggctta tgtggacctg cccaacggca tgatttcagc gctgggcaag
     2101 accgcgacct ttatgatatg gtttacctac aagagtgccg ataccggcga ctggccgcgg
     2161 ctgtttgatt tcggtacgtc aaccggcggc gaaggattct ccaccggcgg cgacggtgtt
     2221 cagtggctca acggcgtgcc gtatctggat acggcggccg aagatacgca ggaatacatt
     2281 atgctctgtc ccaagagagg ctccggcccc ggctggcggt atgaatcact ggttcgtccg
     2341 ccggacaacg gcgtcattat tgatccggca gacaactccg caattgccgg cggcaacgag
     2401 gcctgcatcg cctgcgtctt cgacggcgtc aatcgtgtga tgacccttta tgttaatggt
     2461 gttcaggtcg gtcagaatac gaatttgacc catgatctgg cgaacatcaa tgatgtgaat
     2521 aactggctgg gccgttcgca gtggccggat gctatgttta cgggcaagat taacgagttc
     2581 cgtatttaca acatcccgct gaccaagcac tggattaaag cgtactatga gcagggaccg
     2641 gatgattata caaccgtgcc caatccgtgc attcaggatg cccccaatcc gatggatttc
     2701 aacgccgact gttttgttga tttctcggac tttgtggtct ttgctgcaca gtggctcgat
     2761 tgcgatcgcc tcaatgggtg caatcagtag tcagttgtac caattctgga agacccgctg
     2821 acggcgggtc ttcctttttt gggaatatca cattacatat gagcgagtaa tacaaaaagg
     2881 gggttgtccc tgaacagaac agcccctttc ttttagcatc cggcaggcat ggggtggatt
     2941 ttcagcagaa aagcgcaaac gcgtgcctgg ttatgaggaa gaaatgaaaa acagaagtgt
     3001 tttgctgtct ttattatttc ttatcagtgt tatttggccg acgtccgagt gtgccgcttg
     3061 gggggctgtt gtggcgtact ggaggtttga agaaggacct cccggtgatg gggtgccgca
     3121 cggcgcgtct gtcggagtct ggagtccgga tattctggat tattccggaa acggcaatca
     3181 cctgtctgtg tggtcaacgg gcggatatgc aggatatgcc tggacatcgg atgtacctaa
     3241 accagcaatt ccgacaacag gccagtcgaa taccctgagt gtcaaaaaca ccggcgccta
     3301 tcccggtatg tggactcaaa cgggttccgg cataagcaca atgtccccgt cggcatttac
     3361 gattgaagcg tcgtttaagc tggaaaacgg ggactatcgc acgattgtcg gacgcgacag
     3421 ttacggcact gcctccgtta atgccaattt agccgccctc tattttcagg ccctgcccaa
     3481 caacgctttg gcaattaaat tctgtgatgt atcgggcttc tggcatcagg cggtctcggc
     3541 ggaaggaata tttcagagct ttgactacag caccaatccc agcggtgaag gcgttccatg
     3601 gtacagcatg gcagccgtca gtgacggttc cactctgtcg ctttacctgc tggaacatgg
     3661 ccgggatacc ggctatcagt taattgcgcg tacagatatg accctttccg gaagccccag
     3721 cacggtcttg actgccggag ccggcgacgg cagcgactgg gatgcgggca attggtctgt
     3781 cggacgcggc ctttataacc gtgcgcatac agaccgtgcc tatggattta ttgatgaagt
     3841 acgaatctgc aacagggctc tgcagccatc ggaatttctg ttttatgagc atgttccgca
     3901 aggccttgtt atatcgcccc aaaatctgac tctcagcgaa caggatgccg ggcaggggga
     3961 tctgttcttt tcgctggaat atccgcccgc cggcacagta gtgcttgaaa tttcggagtt
     4021 gtacggccgc gggcagatta tcctcgataa aacaatgttg acatttacgc cggccgactg
     4081 gaatatcccg caggccgttc gcatcacagc ggtggacgat gccgatgtag aaaatgccga
     4141 gcatccggtg ctgctgtcgg ttgttgtttc aagttcacag gatccgttgt acgacggctt
     4201 ggaggtagag ccggttagga tcacagtggc ggataacgaa tgcggcgctt ggggctatgc
     4261 ccccggtgat tacaaccggg attgcatcgt aaatctctat gattttgtta catttgcccg
     4321 atattggatg gattgctccg atcccgagcg gataggatgt accaactttt atccataaaa
     4381 ttatcaataa cagcggctgt tatgtgtagt caaaggattc cagcgatgaa taaaatgaat
     4441 caagggaggg gagttttact aattgttttg gcgttgggtt ttgccggtcc gctttttgct
     4501 tggacgcttc agcaggcgcc ggttatgacg caatgggcat atcaggtaaa tcccgctgag
     4561 ccgctgccgg aatatccacg gccgcagatg gtgcgtccgg agtggatgaa tctgaacggc
     4621 atctggcagt tccagccggg cactgctggc gatacggttc cggtcggtca agtcctttcc
     4681 caagatatac tcgttccttt tccggtcgag tcggcaattt ccggcatcat gcagcattat
     4741 gaccggctgt ggtaccggcg gctgtttgaa atcccgtctc gctggaacgg ccagcgcatt
     4801 ctgctccatt ttggagcggt ggactgggaa accgaggttt atataaacgg cagcagtgta
     4861 ggcatacaca aaggcggcta cgatgccttt tcctttgaca tcacgccttt tctgctgccg
     4921 gctggccagc aggagttgat tgtacgcgtg tacgacccaa cggacaacgg cggctacccg
     4981 cggggcaagc agacgctttc tcccggcggt attatgtata ctcctacgac cggcatctgg
     5041 cagacggtct ggctggagcc ggtgccgcag atttcgctta cggaactgaa gctgattccg
     5101 gatgttgacc ataaattact caacatcaca ggatgggttt cccaacccgc tgccaatctg
     5161 acaattcagg caacggcata tgataatggc gttccggccg gaactgtcag cggtgctgtg
     5221 ggggaccagc tgcagctggt gatgcataat cccaaactgt ggtcgccgga aactcctttt
     5281 ctgtatgatt taaaggttgt tttgaagcag ggttcgacgg tgattgacca agttgacagt
     5341 tattttggca tgcgcaagat tgccttgggg actgtggacg gcgttgtgaa aatgctgctc
     5401 aacgatacgt ttgtgtttca gatgggtccg ctggatcagg gtttttggcc ggatgggatt
     5461 tataccgctc ccaccgatgc agcgctccga tgggacttgg agcagaccaa agcgcttgga
     5521 ttcaatatga cccgcaagca catcaaagtt gaaccggcac gctggtatta ctgggctgac
     5581 aagctgggcc ttctggtctg gcaggatatg cccagtgtca attcctatac cagcaatcct
     5641 cagccgattg ataaagcgca atttaagacc gaattaacgc agatggttcg ccagcactgg
     5701 aacagcccgg cggtgattat gtgggtgatt ttcaatgaaa gtcagggcca gcacgacaca
     5761 gcgccgctgt gtgcaatggt caaggcgatg gatcccagcc ggcttgtcaa tcaggccagc
     5821 ggcggcagcc attacggggc cggggatgtt ctggatatcc acaattatcc cccgccgtct
     5881 tatcctgtca gctcgacaca ggccagagta tgcggcgaat acggcggcat cggaatgcgg
     5941 atttccggcc atatgtggaa tccgaattcg tggggttata caatggtaaa ctctgccgaa
     6001 gagctggcgt cagtgtacga ttcctattcc cagcagttga tttcctttaa gcaaaatcag
     6061 ggtctcagtg ccgcagtcta tacccagatt acagacgtcg agattgagat taacggattg
     6121 attacttatg accgtgcggt tgttaaagcc gatgccggcc agattcggct gtcgaattta
     6181 atgtatgccc ggacctatca ggatgtgctg ccgacatccc aacaaacccc gcagctctgg
     6241 cgctacacga ccagcacccc gccgtccaat tggtctgccc cgtcgtttca tgatgcatcg
     6301 tggaccgaag gtcccggcgg tttcggcaca gccggcacgc cgggagcggt tattggaact
     6361 gtctggaata cttccgatat ctggcttcgc cggatgttta atcccggtcc gttgacgccg
     6421 gcagatttgg accgtctggt ctggcggatt catcatgatg aagatgccga ggtctatatc
     6481 aacggcgcac tggctcttgc aacaacgggt tatacaacca gctatatccc cttgcctgcc
     6541 gctgcggagg ccaaggccgc gctggtgccg aacgccgata atgtcattgc agtccattgc
     6601 cgtcagacaa tcggcggcca gtttatcgat gtcggattgg cactggaaac gattcatgct
     6661 tcctccggtg cctgcggaca gtggggctat gcgccggcgg atctgaatta tgattgtgct
     6721 gttgacatcg aagatttggc tctctttatc gctgattggt ggacctgttc actgcccggc
     6781 cggctggagt gcatcaatta tttactgccg tagcagataa aacagtactt acactattat
     6841 tactgcaagc aaaggaatgt tgccaatggt caagaacgga aagcatgtag aaacaatgga
     6901 aatcgccctt cgtatcatca agcccggcag acagcagctc attggattgc tgatgctgtg
     6961 ccttttgcct ttgccggcac aatcggcttg gatgcctgcg gttcgaccgc ctgcggttcc
     7021 tctggtggcc tgtgatccgt atttcagcat ctggtcctgt gcggatgagc tgacggcgga
     7081 tacaacacgc cattggaccg gaaaaccgca tccgctgaca tcactgattc gcattgacgg
     7141 cagaacctat cggctgatgg gaaacaaacc cgaaggattg cccgcactgc ggcaaacatc
     7201 ggtaaccgtg ctgccgacac ggactgttta tgcatttgaa ggcgaagggc tgtctgtgat
     7261 aatgacattt ctccagcccg ctctgcccga tgacatcgac ctgttgagcc ggccgataac
     7321 ctgtctgact tggcagtgtg cttcagccga tgggaaggcc catgaggtcg ctgtctactt
     7381 tgatgccggg atggaacttg tagtcaatga tccatcacag gctgtggtct ggtttgaaga
     7441 aaatagccct gatatggttg tcctcaaagc cggctccaaa gatcagaata tactcggacg
     7501 gaaaggagac gatgtccgta tcgactgggg atatttttat ctggctgccg caaaaagtga
     7561 ccatccggtc ggtgcaatcg cttcgccgga tgtctgccgg cagtcatttg cccagcaggg
     7621 acgcctcacg caggctatcg acagtcagct gccgcgggaa gtccgcgata acagccctgt
     7681 aatggcaatg cagtttgatc tcggcagaat cgctgcggtc ccgaagacct gccgggtaat
     7741 tttgtcttac gatgatatgc agtcgatcct gtatttcggc cgccggctcg aggccttctg
     7801 gaaaagagat ggcaagcaga ttgctcagat catcaaagag actgcggaga attattctgc
     7861 cttagcgcgg cgatgtgagc aattcgatca ggagctgctg aaggatttgg agcaaaccgg
     7921 cggcccggat tatcagatca tcggggcttt ggcttatcgg caggccctcg gcgccaacaa
     7981 gattgttgcc gataaaaaca agatgcctct gatgttcagc aaggaatgct tcagcaacgg
     8041 ctgcatagga acggttgatg ttttttatcc gtttgcgccg caggttttgc tgctcaatcc
     8101 aaccttagcc aaagcatcat ttgtgccgat tcttgaatac gggcgttccc agcgatggaa
     8161 attcccgttt gccccccacg acatcggcac ttatcctcac gcaatgggac aggtttacgg
     8221 cggcggcgag aggtcagaag aaaatcagat gcctgtggaa gaatgcggca atatgattct
     8281 tctggtggcg gctgtagcgg aggcggaaga tgacattgcc tttgccaaac agtactggga
     8341 tgttcttact acttgggccg aataccttaa aagcaaggga ttggaccccg agaatcagtt
     8401 gtgcacggat gattttgccg gacatttggc ccataatgta aatctgtcgg tcaaggctgt
     8461 tgtcgccttg ggggcgtacg ccaaattggc cgagaaaatg ggcgaccccc aaaccgccgc
     8521 agcatatcga agcaccgccg aacaatatgt acaggactgg atgcgaatgg ccgacgacgg
     8581 cgaccattac cggctggctt tcgacaggtc cggcacatgg agccagaaat acaatctggt
     8641 ctgggaccgt attctcggat taaacctttt cccgcctgca gtagcgcaga aggaaatgga
     8701 gttctataaa agaattcaaa agccctatgg actgccgctg gataaccgca gcgattatac
     8761 aaagctggac tggattgtct ggacagcatc cataaccggc aggcaggacg attttgaagc
     8821 tttgattgct ccggttgtga agtttatcaa tgaaacaccc gacaggattc ctctgaccga
     8881 ctggtattgg acgcataatg ccaaacatcg gggttttcag gcacgccccg tcgtcggagg
     8941 agtatttatc cgcctgatgg ataaccgtca gatttgggaa aaatgggtca gcagagccga
     9001 ttcaatcaga ggcacatggg ctcccttgcc caaaccgccg cagattatcg atgtggttcc
     9061 tgtttcgctg gaaaacggct atcgttggcg atataccttt tcacagcccg atgataactg
     9121 gtataaaccc gagtttgatg ctgcatctgc cggctggaag gagggggctg ccggtttcgg
     9181 cactccccag accccgagca ccgaagtacg gacacgatgg aatacatcag acatctggat
     9241 ccggcggcaa tttacacttg aacaagatgc actggacaat ctttgtctga tgattcacca
     9301 cgatgaagat gctcaaattt atatcaatgg cactttggcg gctgttctta caggatatac
     9361 aacaggttat cagccgtatc cgctgacaga ccaagcccgc agtgcgctga aaacgggtct
     9421 taatacaatt gcaatccatt gccgacaaac cggaggcggc caatatatag atgccggctt
     9481 tgtaaaagtt atcccccaag aataggtcta tggcgaaatc atttttttta gtcagaccgc
     9541 tgacttcgga aacagaccgg caggggttag ttggttcaat atcaagatga ggattgctta
     9601 cgctgttatt tggcagtaaa gttttataat agcaggaaat cataggaaaa tcgacaaaga
     9661 atggaggcgt ttatggaagc aaaaacgcaa gtcttgtttt tattgttgtc ctgttccctt
     9721 tttgctgcag catggcagcc ggctcaagca cccctgatga cacaatgggc cgaggatgtt
     9781 acaccgaaca atgtctggcc ggaatatccg cggccccaca tggtccgcca gaattggcaa
     9841 aaccttaatg gattgtggga ttatgccgtt gtttcccaag atgcccccaa gcccgatagt
     9901 tgggatggca aaatcttggt tcccttttgc atcgaatcag cccttagcgg cgtaatgaaa
     9961 aaagttcagc ccagtcagtc gctgtggtac cgcagaacat ttcagtcctc actggataaa
    10021 agcagcaaac gtctccttct gcacttcggc gctgtggatt ggcaggcggc agtttgggtc
    10081 aatggcaagc aagtcggccg tcataccggc ggatatgatc cttttacatt tgatattacc
    10141 gatgccgttg tggacgggca aaacgaattg gttgtcaggg tttgggatcc gacagatacc
    10201 ggcagccagc caaagggcaa acaggttcta aggccgggcg gaattatgta caccgcggtt
    10261 acaggcatct ggcagacggt ctggctggag acggttccgt ctttgtatat ccagtcgctt
    10321 aaaattgtcc ccgatgtcga ccgcagtgtt gtgcagatta cagtttcagc ttcgaatacg
    10381 gcagaggcag ccattacagt taaggacaac ggcagaacag ccgctcaagc ccaaggcaaa
    10441 accggtcagc ccatcgaagt caagctcgaa gcttgcaagc tttggagtcc cgattcccct
    10501 catctctatg acttggaaat atcgctgatg gatgcttcgg gtgcggccga ccgcgtcagc
    10561 agttatttcg gaatgcgcaa aatcgaagtc aaaaaggatg aaaatggaat caatcgtctg
    10621 ttcttaaata acaaggtcct ctttcagtat ggacctctgg atcagggctg gtggccggat
    10681 ggcctttata cacctgcttc gcaggcggcc atggaatatg acatcgtgat gaccaaaaag
    10741 ttcgggatga atatggcccg caaacacgtc aagtatgagt gtgcccgctg gtattactta
    10801 tgcgacaaac tgggtttgct ggtctggcag gacatgccct ccggcgatac cggcagggat
    10861 gaggaaagca aatccaactt ccgcacagaa ttgaaggcga tggttgatgc cctgcataat
    10921 ttgccctgta ttgtaatgtg gataccgttc aacgaaggct ggggccagca tgacacagaa
    10981 gaaattggcc aatggatgca gaattacgat cccacgcgtc cgatcaatga ggccagcggc
    11041 tggcatgaca gaggcagcgg cgatgtgtct gatatgcaca actaccccgg ccccggggtg
    11101 cggccggcgg aagacaaacg cgtctgtgtc ttgggcgagt ttggcggttt ggggcttccc
    11161 atcgaagggc atctctggca gccggaccga aactggggct atgtatccta taaaaataca
    11221 gaggagttga ccgatgcata tgtgggactt ctgacagcta tgcgtccgct gattgcgcag
    11281 ggattgtcgg cagcggttta cacccagacc tcggatgtgg agatcgagat aaacggcctg
    11341 atgacatacg accgcaaagt tgtcaaaatg gatcttggca ggtgcgccga agcagctgct
    11401 aaattgtacc gccggccgcc tgctgtcaag acattggttg aaaccagtca gcagcagcgg
    11461 cagacatggc gctataccac tcagagcccc tcaaacaatt gggtggagac ggcatttgat
    11521 gatacatctt ggagaacggg ccgcggcggc tttggaacat ccggtacacc cggcgctgtt
    11581 gtgggaacag tctggaatac ctctgacatc tggatgcggc gaacatttga actggataca
    11641 gttcctgcac aaggggatct gctgctggtg attcatcacg atgaagatgc acaagtgtat
    11701 ctgaatggaa agcagatcaa gacattgtcc ggatacatca caaattatac atttgccccc
    11761 ctttctcccg atgcactcaa attgctgaaa accggcgtca atacgctggc tgtccattgc
    11821 cgtcagacca gcggaggaca gtatatcgat gtaggactgt cgcttctgct ggattagccc
    11881 caaacaagcg gcgaccgctg cgtttgcccg ctgagcagcc gcaggcatgt tcgatataca
    11941 aatactatct gcaaaaggga aacacgatga agcaatgggc actgttgctg ggtattgtcg
    12001 gcacggcttt ggcggccgag caaattaccg tggtggaccg ccccgatgtg cattctgtca
    12061 atagtttcta tgtcagcaac cgtccgcctc tggagccgag ccgattgatt gaattgcctg
    12121 tgggctcaat taagccggcc ggctggctgc tgacctgtct gcggcggcag agagacggct
    12181 taaccggcaa tctgggcaac attagcgcct ggcttcaaaa agaggacaat gcttggctca
    12241 gtctagatgg caaaggaaga tggggctggg aagaagtgcc ctattggctt aggggctacg
    12301 gtgaactggc ctatattctt gacgatcccc agatgattgc cgaaaccaaa atctggattg
    12361 aaggagccct tcgcagtcag cgggatgacg gtgatttcgg tccggatcaa agattcaatg
    12421 acgggacacg ggactactgg gcgaatatgc tgatgcttta ctgcctgcag tcctactacg
    12481 attattcagc agaccagcgt gtactggatt tgatgaggcg gtacttcagg catcagtctt
    12541 ccgttccgga tgaacggctg ctgaccggct attggcagcg gatgcgcggc ggcgataacc
    12601 tctacagcat ttactggctc tacaaccgca ccggcgaaaa atggctgctg gaaaccgcag
    12661 agaagattca ccgcaataca gccgactggc gcatggaagg ggatttgccc aactggcaca
    12721 atgtcaatat tgcgcaggcg tttgatgagc cggcggtatg gtttctgcag agccgaaatc
    12781 ctgctgattt ggagtttgcc tataaaaact tccatgaagt gcggcggcgc tttggtcagg
    12841 ttcccggcgg catgttcggc ggcgatgaaa actgccgggc cggttatgat gacccgcggc
    12901 agtgcattga aacctgcggt atgattgaac aaatgctgtc ggatgaaatg ctggtgcgga
    12961 tttccggcga tcctttctgg gcagaccact gcgaagaagt ggccttcaac agctatccgg
    13021 cagccctgat gcccgatttt cggtcgctgc gctatctgac tgcccccaat atggcggcca
    13081 gcgaccgcca caaccatcgt ccgggcattg acaacggcgg gccttttctg ttgatgaatc
    13141 cgttcagttc gcgctgctgc cagcataacc attcccacgg ctggccgtat tatgtgaaaa
    13201 atctttggct ggccacgccg gataacgggc tttgtgcatc gctgtatgcc gccaacgaag
    13261 tcagggccaa agttggcgac ggaacagaaa ttaccctgcg tcaggagaca cattatccat
    13321 ttgaccagca gatacgcctg acgctgttgt ctgaaaagcc ggttcggttt ccgctctatc
    13381 tgcgtgttcc gggctggtgt gaaaacccgg ccgtgcaaat caacggtatg ccccgttcta
    13441 tagcggcaca gccgcggcag tacattcgct tggagcgggt atggaccggc ggcgatacgg
    13501 tcgttctg
//
LOCUS       DAOTBO010000076        13307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_3911_length_13307_cov_5.379943, whole genome shotgun sequence.
ACCESSION   DAOTBO010000076 DAOTBO010000000
VERSION     DAOTBO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3911_length_13307_cov_5.379943"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLE88_09090"
     CDS             <1..607
                     /locus_tag="PLE88_09090"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOM76656.1"
                     /translation="EAVKDELAQKIMVIGEDIPLPIETATDDPFQVGTDRLVTAAAAY
                     SVIEDAVVVADFGTAVTVDLVDENGVFLGGTISPGFDLSMKAMKAGTARLPQVAMQKP
                     LDVYGANTEEAMRAGVYWSAVGLLETVCRKYAEQLGKWPHVILTGGAAGLIRDDCTFV
                     DSYVPDLAVRGIMIAYKKYLYEKAHIAELDAEKPIKPPAEE"
     gene            610..1242
                     /locus_tag="PLE88_09095"
     CDS             610..1242
                     /locus_tag="PLE88_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOM76657.1"
                     /translation="MACPDNLVREFVEACHKVAALGLVQCSSGNMSRRIGPDTALLTA
                     SRSWLAEITDQQVAICGIQTGQCLNGKIPTCESVFHLGILKNRPDVNVVLHFQSPYAT
                     ALACGNPLKWNYNVIIEVPVYIGIPAAVDYFPPGSPELAQAVIEEMKNPQTQLAFMKN
                     HGLVAVGVDFNDVIQKAVFFELACQILLTNPNARPLPQEAADALRRSSKA"
     gene            complement(1429..2487)
                     /locus_tag="PLE88_09100"
     CDS             complement(1429..2487)
                     /locus_tag="PLE88_09100"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOM76658.1"
                     /translation="MNKASEQFLKELLETPSPSGFEQPAAKLWRAYVKDSVDELSGDV
                     HGNSIAVLNPKAPLKFMLAGHIDEIGLMITHIDEKGYLYVAQIGGMDPALLIGQRVAI
                     ASEKGPVFGVIGRKAIHLMTPEERKKSVEMDDIWVDIGANSRKDALKRVAIGDPMVVD
                     VVCRRLDDDKLVTRAADDKAGAFVVAECLRALAGRKLNVCVVGVATVQEEVGLRGAIT
                     SSYSIKPDAGIAIDVTFASDHPDTNPKKTGEVKLGKGPVLHRGPNINPIMERELFKTA
                     KSRKIAYQVTAEPRGTGTDANAMQLARGGAAVGLISIPNRYMHTPVEVISTKDLDNTI
                     KLIVEYIAAHPAKRDYRP"
     gene            2686..3477
                     /locus_tag="PLE88_09105"
     CDS             2686..3477
                     /locus_tag="PLE88_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOM76659.1"
                     /translation="MKHRLQIAAALIALAGMMLAGCSTQKLTCSQVASQLAVSSNKAI
                     KPVPRSNDWWKQRHDAVVERVKQGNVGILFIGDSITHGWDSQKELWDASFGRWNPVNA
                     GFSGDRTEHVLWRLENGEIDGIQPKAAVLMIGTNNSNGDEYTAEQIAEGIQAIVCTLR
                     TKLPQTKVLILAIFPRGSREQMADKTQNGSYNPQWAKNDQASRLASQLADGKWIYYLD
                     INKAFLNENGVLTREVMPDLLHPKEKGYQIWAQAMLPTLEKLMAD"
     gene            complement(4030..4302)
                     /locus_tag="PLE88_09110"
     CDS             complement(4030..4302)
                     /locus_tag="PLE88_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76660.1"
                     /translation="MLSHPLTFLTGYLAGAFALSFGIIGPLAALAAEFFGGRRTDRQY
                     LKGLRAGISGTCLAFIVLHYLLYLRGYGITDWSAVWSDLFYPPSVF"
     gene            complement(4369..4533)
                     /locus_tag="PLE88_09115"
     CDS             complement(4369..4533)
                     /locus_tag="PLE88_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HOM76661.1"
                     /translation="MKLVKIILAIFLPPVAAFLQVGVGLHFWLNILLTLCFGLPGMIH
                     ALWLVAADKH"
     gene            4712..5617
                     /locus_tag="PLE88_09120"
     CDS             4712..5617
                     /locus_tag="PLE88_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOM76662.1"
                     /translation="MSKPEILGIGLGLGAAFFQSLSYLCSRLFMKRHQDNILALLALS
                     HIAMGIVSLAAAVLLRPAAMPALHCYLKALLGASGFYLLGQFFLFAFITRTEPSRISP
                     LLGLKVFMLAVIGAAFLGQQFQTAQWLAVLCSTLSVFLLSYAGGRLEWPFVGMGLLVC
                     LFYCLSDINIKILVDYFGFMGPIRGAAMATALCYIFCGLVGAGVLLFRPSCSTRQTWL
                     YAAPFAASWFIAMILLFSSFGLIGVVFGNIMQSVRGIFSILLAFAVAHLGFEALEPKA
                     SARQIIQRTLAAILMSASVALFLFS"
     gene            5763..6245
                     /gene="greA"
                     /locus_tag="PLE88_09125"
     CDS             5763..6245
                     /gene="greA"
                     /locus_tag="PLE88_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOM76663.1"
                     /translation="MHEIMPISQTGYKKLREELEQLEHQAAELRQRVAEAREQGDLRE
                     NAEYIYGRQNLGFIEGRMGEIRGKLNYSKVVDCTQVPSDKAGFGTVVTLLDLDTKKKV
                     TYQLLGPYDSDLTDDSISILSPIGEALVGLAAGDKVSVDVPRGRLHFEILEIAKSPIQ
                     "
     gene            complement(6310..7500)
                     /locus_tag="PLE88_09130"
     CDS             complement(6310..7500)
                     /locus_tag="PLE88_09130"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOM76664.1"
                     /translation="MSKVLIIGAGGVGNVVVHKCAQQPDVFSQIVLASRTKAKCDKIA
                     AALKRPITTEQVDADSVPQIIALIHKHRPDIVINTALPYQDLPIMDACLDTKTDYLDT
                     ANYEPKDEAKFEYKWQWAYHDRYQKAGIMALLGSGFDPGVTNVFCAYAQKHFYDEIHT
                     VDIVDCNAGQHGHPFATNFNPEINIREITQKGKYWENGRWIEIEPMSVSMDIDYPEVG
                     PKRSYLLYHEELESLVKHMKGLRRIRFWMTFGQAYLTHLQVLQNVGMTSIKPIDFKGQ
                     KIVPLEFLKAVLPDPGSLGTTYSGKTCIGCIFDGVKDGKQKQMMIYNVCDHAECFKET
                     GSQAISYTTGVPAMIGAKMMLTGKWKRPGVWNLEQFDPDPFMDDLNRCGLPWKVLDGK
                     TLEG"
     gene            complement(7785..8108)
                     /locus_tag="PLE88_09135"
     CDS             complement(7785..8108)
                     /locus_tag="PLE88_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76665.1"
                     /translation="MKNGHTIILIAVAILVLLSLLGCSETSRKAAAERRWDRTLERAR
                     LEAAQAGIKEGRLAYARHILEDCIESQSSCAEQAAAILAELQKTEQQIAQARLNTSAD
                     QSRIN"
     gene            complement(8581..8859)
                     /locus_tag="PLE88_09140"
     CDS             complement(8581..8859)
                     /locus_tag="PLE88_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76666.1"
                     /translation="MFRFFFHSNPFRQITKDLARSSFISGLILIGLGMLVWVLKDIFA
                     IIAMVIFFAAGFSAIGYAARLYLMQWKMRKDSRVYRKGVEIHFEEDIF"
     gene            complement(8920..10725)
                     /locus_tag="PLE88_09145"
     CDS             complement(8920..10725)
                     /locus_tag="PLE88_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOM76667.1"
                     /translation="MQTVELLAPARDFEAAQAAILCGADAVYIGAEQFSARQAAGNSI
                     ETIRRVIELAAPYYVRVYAALNTLLTDDELPQAGRLIEQLYEAGIDGLIIQDTGLLEL
                     DLPPVPLIASTQMNNTTIDHIRFLQQVGFSRVILARELSLEQIKQIRQAVSIELECFI
                     HGALCVGQSGQCWLSYALGGRSGNRGQCAQPCRRLYTLKDSQGRTIVKNRYLLSLKDL
                     NLSAHLEALLDAGVVSFKIEGRLKDAAYVANTVGFYRRLLDGILAKRGLQKSSSGICR
                     LNFEPDPQKTFSRGFTDYGINGRIDSFGCIDTPKSIGSCVGIVQQVHRDSFVLDSSVQ
                     LHSGDGLCYFDEDKSLTGTTVNRAEGQTVWPQKIGSLKVGTAVYRNYDHRFHKQLQRL
                     GERKIPIQMTLQDTDNGVALAGVDQEGNQAHFSLPLDKQPAQKTQQARQTAAKQLQKL
                     GGTIFICNDVRIETQQIYFLPVSVLNALKRGLTAALVQTRLTGRPRRAGGIVKNSVPY
                     CRKRLDFRGNVLNEKAQAFYKRHGVEHIEPAAESGCDLHNEAVMTTKYCLRKQLNLCP
                     QEGQAEPLLLEDEDGHLFQVQFRCGRCGMDIIWIK"
     gene            complement(10791..12410)
                     /gene="groL"
                     /locus_tag="PLE88_09150"
     CDS             complement(10791..12410)
                     /gene="groL"
                     /locus_tag="PLE88_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOM76668.1"
                     /translation="MAAKKIAFDTEARAAIREGIRKLAAAVKVTLGPCGRNVILEKSF
                     GSPTVTKDGVTVAKEIELEDPYENMGAQMVKEVASKTSNVAGDGTTTATILAEAIFEE
                     GLKNITAGANAMQVKRGIDKAVEAIVAELQKMSVKVDSAKQIEQVATCSANHDAEIGR
                     TMAQAMEKVGKDGVITVEEGQSLETTVELLEGMQFDKGYLSPHFINNPEERTVVLEKP
                     YILIHEKKISAVKSLVPILEKVAKQGRPLLIIAEDVEGEALATLVVNKLRGVLQVCAV
                     KAPGFGDRRKAMLQDIAILTGGQALFEDLGLQLENVELSQLGQAKRVTVDKDTTTIVE
                     GAGKTSDIKGRIEQIKKEIEASTSDYDIEKLQERLAKLAGGVAQINVGAATEAEMKEK
                     KARVEDALHACRAAVEEGILPGGGVSMLRCLPALDKIKCAGDEQIGVDIVRRAVVAPI
                     KQIAANAGLDGSIVAQKVMESSDKNFGYDAVRKEYGDMIKFGVIVPTKVERTALQNGA
                     SIASLLLTTDAIVSEIPEKKKTPAAPPSDMY"
     gene            complement(12458..12748)
                     /gene="groES"
                     /locus_tag="PLE88_09155"
     CDS             complement(12458..12748)
                     /gene="groES"
                     /locus_tag="PLE88_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOM76669.1"
                     /translation="MKIRPLGDKVIVQRLEAEAKTAGGIVLPDTAKEKPRRGKVISVG
                     EGKLLDDGKRAEMSVKKGQEVLFGSYAGTEVKIDGKEYLIMDESDILAIIEE"
BASE COUNT         3114 a         3507 c         3267 g         3419 t
ORIGIN      
        1 cgaggccgtc aaagatgagc tggcccagaa gattatggtt atcggagaag acattccgct
       61 gccgattgaa acagccacgg atgatccgtt tcaggtagga acagaccggc ttgttacagc
      121 cgcagcggcc tattcagtca ttgaagacgc tgttgtggtg gccgatttcg gtacggcagt
      181 tactgtggat ttagtggatg aaaacggtgt ttttcttggc ggcacaatct cgcccggctt
      241 tgatttgtcg atgaaggcaa tgaaagccgg cacagcccgc ctgccgcagg tggcgatgca
      301 gaagccgctg gatgtgtacg gcgccaatac agaagaggcg atgcgtgcgg gcgtctactg
      361 gtcggctgtc ggtcttctgg aaacggtctg tcgaaagtat gccgagcagc tgggcaaatg
      421 gccgcatgtg attctaaccg gcggggcggc tggcctgata cgcgacgact gcacatttgt
      481 ggattcctat gttccggatt tggctgtacg cggaattatg attgcctaca aaaaatacct
      541 ttatgagaag gcccacattg cggagctgga cgccgaaaag cccataaagc cgccggctga
      601 ggaatagcga tggcttgtcc ggacaatctg gttagggagt ttgtcgaagc ctgccacaaa
      661 gtcgccgcac ttgggctggt gcagtgcagc agcggcaata tgtcacggcg catcgggccg
      721 gatacagccc tgctgacagc cagtcgttcg tggctggcag agattaccga tcagcaagtc
      781 gcaatctgcg ggattcagac aggccaatgc ctcaacggca agataccaac ctgcgaaagt
      841 gtctttcact tgggaatctt aaaaaacaga cctgacgtca atgttgtgct gcatttccag
      901 agtccttatg ccacagccct cgcctgcgga aatccgctaa aatggaacta taatgtcatc
      961 atcgaagtac cggtttatat cggtattccg gctgcagtgg actactttcc ccccggctcg
     1021 ccggaattgg cacaggcggt tattgaagag atgaaaaatc cgcaaacaca gctggcgttt
     1081 atgaaaaatc acggtttggt tgcggtcggt gttgatttca acgatgtgat tcagaaagca
     1141 gttttttttg aactggcctg tcagatttta ctgaccaatc ccaatgcccg ccctttgccg
     1201 caagaggcag cagatgcact gcgccggtcc agcaaggcct aggcaagcca aaatgctttc
     1261 cggcacttca ttggtctgct ggatttttga aagatatgct gtgcaatttc agaacacttg
     1321 cccgagagtg cggtttttct gtcatcaaag ctaaaaagca gcgtccctca gagcccaatc
     1381 atctacagcc ggactataag gcgtcaggaa gaaatcaaaa gaatcacatc agggtcggta
     1441 atcccgcttg gccggatggg cagcaatata ttccacgatc agcttgatag tattgtccag
     1501 atccttggtc gagatgacct ctaccggcgt atgcatatag cggtttggaa tgctgataag
     1561 tccaacagcc gcgcccccgc gggcaagctg catagcattc gcatcagtgc cagtgccgcg
     1621 cggttcggct gttacctgat aggcaatttt tctggatttg gctgttttga acagttcccg
     1681 ctccatgata ggattgatat tcggtccccg atgcagaacc ggtcctttgc ccagcttgac
     1741 ttcaccggtc tttttgggat tggtatccgg atgatcgctg gcgaaagtta catcaattgc
     1801 aatccccgca tcgggcttga tggagtagct ggaagtaatc gcaccgcgca ggccgacttc
     1861 ttcctgtact gtggcaacac ccactacgca gacgttaagc ttgcggcctg ccaaagcccg
     1921 caggcattcg gcgaccacaa atgcaccggc cttgtcgtcg gcggcacggg taaccagctt
     1981 atcatcatcc agccggcggc agacaacatc caccaccatc gggtcgccaa tggccacacg
     2041 cttgagagca tccttgcgcg aattggcgcc gatatcgacc caaatatcgt ccatttctac
     2101 gctctttttg cgttcttccg gtgtcatcag gtgaatagcc ttgcgcccga tgaccccgaa
     2161 aacgggtccc ttctcggagg caatggcaac acgctggcca atcagcagcg ccggatccat
     2221 ccccccgatc tgggcaacat aaagatagcc cttctcatcg atatgtgtaa tcatcagccc
     2281 aatttcatca atatggccgg ccagcataaa cttcagcggc gccttgggat tcagcacggc
     2341 aatgctgttg ccatgcacat caccggacag ttcatcgaca ctgtctttga cgtatgcccg
     2401 ccagagtttg gccgccggct gctcaaagcc cgacggactg ggcgtttcca gcagctcttt
     2461 taagaactgt tcagatgcct tgttcattat tttcatcctg catacagtga gttctggttt
     2521 ttgctgttta ttatatgaat ccctactccc aatgcaaacc gaaaatcaat cctgtccgaa
     2581 caggtgctct tctatttgcc gattattcaa aatcacagtt gactttgaca tctggattta
     2641 acacaataca ggcagacaaa acctcttttt ggaaaggact atggtatgaa gcatcgattg
     2701 caaattgcgg cggcactcat cgctttagcc ggtatgatgt tggccggctg ctccacacaa
     2761 aaactcacgt gcagtcaggt tgccagccaa ctggcggtgt cgtccaacaa agccatcaag
     2821 cctgtgcctc ggtccaatga ctggtggaaa cagcgccatg atgctgttgt ggaacgggtc
     2881 aaacagggca atgtcggcat cctcttcatc ggcgattcga tcacgcacgg ctgggacagt
     2941 cagaaagagc tgtgggatgc ttcgttcggc cggtggaatc ccgtcaacgc cggctttagc
     3001 ggcgaccgca ccgaacatgt cctctggcgg ctggaaaacg gcgaaatcga cggcatccag
     3061 cctaaagcgg ctgttctgat gattggcacc aataattcca acggggatga atacacagcg
     3121 gagcagattg ccgaaggtat tcaggcaatt gtctgcacgc tgcgtaccaa actgccgcag
     3181 accaaggtgc ttattctggc gattttcccg cgcggatcga gagaacagat ggccgacaaa
     3241 acccagaatg gttcctacaa tccccaatgg gccaaaaatg accaggcaag caggctggcc
     3301 agtcagctgg cagacggcaa atggatttat tacctcgata tcaataaggc gtttctgaat
     3361 gaaaacggtg ttctaacccg tgaagtgatg ccggatctgc ttcaccccaa ggaaaagggc
     3421 taccagatct gggcccaagc aatgttgccg acacttgaaa aattgatggc agactaaatt
     3481 tttcaccatc tgcagcactt atcaaagatg catccctgaa gagggatgca tcttttttta
     3541 ttgagatagc ctttgtcttc cggcaggatg agcgcaaaat aaaagccaga ttcagcatgt
     3601 gaacctggct tcggaggagg gtgatgagaa gaaattcgta tgattattgc ggctttcccg
     3661 ctcctctcta cacttataaa cgtttgaaaa agaaaaatgt tttcaaaatt cagaaatttt
     3721 ttatctttcc cagaaggcgg cagtattgta gccccgtatt tgtgtaattc aagaaaaatt
     3781 ttagaaaaat attaaaaaaa tgttaaaaat atttgagact tgatttatat cagacaataa
     3841 gggtatatat atatttaaaa agaacaatat aaaccattat atttactcaa aaaggccaat
     3901 attataggtc gtcataaaat gcataaccgc ctctttctta tgtcgatact atgacaagtc
     3961 agccatttca cacaagtcaa ctgttaaaaa tgcccttctt aaagtcctat ttagaaatgg
     4021 ttatgccccc taaaatacag atggagggta aaaaagatcg ctccagacag ccgaccagtc
     4081 cgttatgccg taaccgcgca gatataaaag gtagtgaagc actataaagg ccagacaagt
     4141 tcccgatatg ccggcgcgca gtcctttcaa atattgccta tcggtgcggc gtcccccaaa
     4201 aaactcggct gccagagctg ccagcgggcc gataatcccg aaagacaaag caaacgcccc
     4261 ggccagatag ccggtcagaa atgtcaacgg atgcgacagc atacgtaaac tccttacaaa
     4321 ttaatacccg aagccgccgc cgcagttata ggccgcctgt tggcttgttc aatgtttgtc
     4381 agcagcgacc agccaaaggg cgtgaatcat ccccggcagc ccgaagcaca gcgtcagcaa
     4441 gatattcagc cagaaatgca aaccgacacc aacctgcaaa aatgccgcaa cgggaggaag
     4501 aaaaatggcc agaatgattt tcaccagctt catacacaat actccttaat tgctctgcaa
     4561 tatgtataaa caaggccgat tttaaacaat ttttttccta tgtctattgc ttttatcggc
     4621 agattgtccg aaaaactgtt tttttgtctg tttttctgct caccagcccg tataatcctt
     4681 tcctgccgaa ttgcaaacga acagaaaaag tatgagtaaa ccggaaatac tgggaattgg
     4741 attgggattg ggcgcggctt tttttcagtc gctgtcgtac ctgtgttcgc ggctgtttat
     4801 gaagcgccat caggacaaca ttcttgctct gctggctttg tcgcatatag cgatggggat
     4861 cgtttcgctg gcggcggcgg ttctgctgcg gccggcggca atgcctgccc tccattgcta
     4921 tctgaaggcg ctgctggggg cttctggttt ttatctgctg gggcagtttt ttttgtttgc
     4981 tttcattacc cgcacagaac cgtcgcggat ttcacccctg ctgggactga aggtctttat
     5041 gctggctgtt atcggggcag ctttcctcgg tcagcagttt caaacagccc aatggctggc
     5101 agtgctgtgc agcacgctgt ctgtatttct tctgtcatat gccggcggcc ggctggaatg
     5161 gccgtttgta ggaatgggtc tgctggtctg tctgttttac tgcctgtcgg acattaacat
     5221 caagatttta gtggactatt tcggctttat gggcccgatt cgcggcgcag ccatggccac
     5281 tgccctgtgc tatatcttct gcggactggt cggtgcgggg gtgctgctgt ttagaccctc
     5341 ctgttcgacc cgtcagacat ggctgtatgc ggcaccgttt gccgcaagct ggtttattgc
     5401 gatgattctg ctgttttctt cttttggtct gatcggagta gtcttcggca atattatgca
     5461 gtcagtacgg ggcatttttt ccatcctgct ggcgtttgct gtggcgcatc tgggttttga
     5521 agcgctggag ccgaaggcgt cggcccgaca gattatccag cgtacgctgg cggcgatttt
     5581 gatgtcggcc tcggtcgcct tgttcctttt ttcctgagag ggaaaaatta agccctgtca
     5641 gcagaatatg tacccggcgg gccgataatt tttttaaaat ctcccgaaaa aaactttcaa
     5701 tgtcaaccgg cttttttata attaggccgc ttttatgaac agattgtacg gagaccggta
     5761 cgatgcatga gattatgccg atttctcaaa cagggtataa aaaacttcgg gaagaactcg
     5821 agcagctgga acatcaggcg gcagagttgc ggcagcgggt ggccgaagcc cgcgagcagg
     5881 gcgacttgcg ggaaaatgcc gaatatatct acggacgcca gaatctcggc tttatcgagg
     5941 ggcgtatggg ggaaatccgc ggcaagctga attattcaaa agtggtggac tgtacgcagg
     6001 ttcccagcga caaggccggc tttggaacgg tggttaccct gctggatttg gatacgaaaa
     6061 agaaggttac ctatcagctt ctgggaccat atgattccga cctgaccgat gacagcattt
     6121 ccatcctgtc gcccatcgga gaggcccttg tcggtctggc ggcaggcgac aaagtctcgg
     6181 tagatgtccc ccgcggccgg ctgcattttg aaattctgga aattgccaaa tcgcccattc
     6241 aataaaggaa cccaccccgc cggttcagcg tcggcataag tgtcgggttc tgcggccgga
     6301 cggtttgatt cagccctcca gtgttttgcc gtccaaaacc ttccatggca ggccgcagcg
     6361 gttcaggtcg tccataaacg gatccggatc gaactgctcc agattccaca cgcccggccg
     6421 cttccatttg cctgtcagca tcattttcgc gccaatcatc gccggcacgc cggtggtata
     6481 ggaaatcgcc tgcgagccgg tctccttgaa gcactcagcg tggtcgcaga cattgtaaat
     6541 catcatctgt ttctgcttgc cgtcttttac gccgtcaaaa atgcagccga tgcaggtctt
     6601 gcccgagtag gttgttccca gcgatcccgg atccggcagc acggctttga gaaactccag
     6661 cggcacaatc ttctggccct tgaagtcaat cggtttgatg ctggtcattc caacattctg
     6721 caacacctgc aagtgcgtca ggtaggcctg tccgaatgtc atccagaatc gaatacggcg
     6781 cagacccttc atatgcttga ccagcgattc cagctcctcg tgatacagca ggtacgaccg
     6841 ttttggaccg acctcggggt aatcgatatc catggacacg ctcatcggct cgatttcaat
     6901 ccagcggcca ttttcccagt acttgccttt ttgggtgatt tcgcgaatat tgatttcggg
     6961 gttgaaattt gtcgcaaacg ggtgcccgtg ctggccggca ttgcaatcga caatatcaac
     7021 agtatgaatt tcatcataga aatgcttttg ggcataggcg cagaacacat tggtaacgcc
     7081 cgggtcaaag ccgctgccta aaagagccat aattcctgcc ttctggtagc ggtcatgata
     7141 agcccactgc catttatact caaatttggc ctcatccttc ggttcgtaat tggctgtatc
     7201 cagatagtcg gtttttgtat ccagacaggc atccataatc ggcaaatcct ggtacggcag
     7261 cgctgtattg atgactatat cgggccgatg tttgtgtatt aaagcaatga tctgcggcac
     7321 actgtcggca tccacctgtt cggttgttat cggccgcttg agggctgcgg cgattttgtc
     7381 gcatttggct ttggttcggc tggccagcac tatctggcta aaaacgtccg gctgctgggc
     7441 acacttatga accactacat tgccgactcc gccagctccg atgatcagca ctttggacat
     7501 cgtttctatc tccctgactg tttcctgttt tgtaccatcg gctgaattta taccacagtc
     7561 ggccgaaaaa tccaatcctt attccgaagc gcaccaagac cgctatcaaa aaaatcaggt
     7621 atccccaaag gaatgggcgg ctgctgagag ggtatacaat ttaggcaaat gttttcaggt
     7681 ttgctttatt ttagatccta tgaaaagaat acactgacta cctcccctcc tttgcctgct
     7741 gcgccgaggc cgagatagcg gcctttggcg ccctcgtttg cggattaatt aatccgcgat
     7801 tggtcagcac tggtgtttag ccgtgcttga gcaatctgct gttcggtctt ctgaagttca
     7861 gccagaatag cagccgcctg ttcggcacag gatgattgcg actcgatgca gtcctccaaa
     7921 atatgccgtg cataggccag ccggccctct ttaatgccgg cttgggcggc ttcgaggcgg
     7981 gcccgttcca aggtccggtc ccagcgccgc tcggcggcgg ctttacggct ggtttccgaa
     8041 catcccaaca gcgacagcag aacgaggatc gccacagcga tcaggataat tgtgtgtcca
     8101 tttttcatag tatccttctt tctcgtcttt ttcgcagcaa tgcagtacat catagtgtca
     8161 tttatggttt tcgatggttt taaaaagcgc aaaacaacgc ttgaactgtt tccttataaa
     8221 accatacgaa ataatttttc gaatgccaaa tccttgtttt gaaggaacac gcaaaaatga
     8281 cctattatcg ctgttttcgg taaatcagaa cgtttttctt tgggaaaata ttgcagaaat
     8341 gacctttggg aaggatattt tttagtatcc tgaaaggata tttttaacgg cttttatatc
     8401 aggtttgccc tgagcgaaat tatcggtctg ttacttgtat aaagccgctg cttgcatttt
     8461 ttaccggcgg gcggctttgc ggtgcatgtg tgttttaagg caaatcccgt caatcaaacg
     8521 gttttttatt gcctgccgac gaaaacgacg aacagaacga ttcgctatcg gtataaggag
     8581 ctaaaagata tcttcctcga agtgaatttc tacacctttg cggtacacgc ggctgtcctt
     8641 gcgcattttc cactgcatta aatatagacg ggcagcgtag ccgatagcgg aaaaacctgc
     8701 cgcaaagaaa ataaccatag caataatggc aaagatatcc tttagtaccc acaccagcat
     8761 accaagaccg atcagaatca gaccgcttat aaaactgctt ctggccaagt cttttgtaat
     8821 ctgccgaaac ggattgctgt gaaagaaaaa acgaaacatt ttctgattcc tttcaatggg
     8881 ttattataca ctatacgcct aaagccggcc gctggtgcat tattttatcc aaattatgtc
     8941 catcccgcac cggccgcatc gaaactgcac ttgaaacaga tgcccatcct cgtcttccag
     9001 cagcagcggc tcggcttgcc cttcttgggg gcatagattc agctgttttc gcaggcagta
     9061 tttggttgtc atcaccgcct cattatgcaa gtcgcatccg ctttctgccg ccggctcgat
     9121 gtgttcaact ccgtgccgct tgtaaaacgc ttgtgccttt tcattgagga cattgccgcg
     9181 aaagtccagc cgtttacggc agtacggcac actgtttttg acaatgccgc cggcccgccg
     9241 cggccggcct gtcagccgcg tctgcaccaa tgccgccgtc agcccccgtt tgagtgcatt
     9301 gagtacagag acaggcaaaa agtagatttg ctgtgtttcg atgcgtacat cattgcagat
     9361 aaagatcgtc ccgcccaact tctgcagctg tttggccgct gtctgacggg cctgctgcgt
     9421 tttctgtgca ggctgcttat ccagcggcag cgaaaaatgc gcctgattgc cctcttggtc
     9481 aaccccggcc agtgcgacgc cgttatctgt atcctgcagc gtcatttgga taggaatctt
     9541 ccgctcgccc agccgctgga gctgcttatg aaatcgatgg tcataattgc ggtaaacagc
     9601 ggtcccgact ttcagagaac cgattttctg cggccaaacc gtttgcccct cagcccgatt
     9661 gactgttgta ccggtaaggc ttttatcctc atcgaaatag caaaggccgt cgccgctgtg
     9721 cagctggacg gagctgtcca gcacgaagct gtcccgatgc acctgctgga cgatgccgac
     9781 acagctgccg atagatttgg gcgtgtctat gcatccaaag gagtcgatgc ggccgttaat
     9841 tccgtaatct gtaaaacccc ggctgaaggt tttctgcgga tcgggttcaa agttcagccg
     9901 gcagatgccg gaggagcttt tctgcaatcc ccttttggca aggatgccgt ccagcagacg
     9961 ccgatagaaa cccaccgtat tggccacata cgccgcatct ttcagacgac cttcgatttt
    10021 gaacgagaca acgccggcat ctaacaaagc ctcaagatgt gctgacagat tgagatcctt
    10081 cagcgacaga aggtagcggt ttttaacaat cgttcggccc tgcgaatcct tcagcgtata
    10141 aagccgccgg cacggctggg cgcactgccc gcgattgccg ctgcgcccgc cgagagcata
    10201 actgagccag cattgcccgc tttgacccac acacaaggcc ccgtggataa agcattccag
    10261 ttcaatcgac accgcttgtc ggatttgctt tatctgctct aaagacagct cccgtgccag
    10321 aatcacgcgg ctgaatccaa cctgctgcag gaatcggata tggtcaatag tcgtattatt
    10381 catctgcgtg ctggcgatca ggggcaccgg cggcagatcc agctccagaa gacctgtatc
    10441 ctgaataatc agcccgtcaa tgcctgcttc gtaaagctgc tcaatcagac ggcctgcctg
    10501 cggcagttcg tcatccgtca gcagcgtatt cagagccgcg tacacccgga cataatacgg
    10561 agcagccagc tcgatgaccc gacgaatggt ttcaatactg ttgccggctg cctgacgggc
    10621 gctgaactgt tcggcgccga tatagacggc gtctgcaccg cacagaatag cagcctgtgc
    10681 cgcttcaaaa tctctggccg gagctaacaa ttcaacggtc tgcattgctc agattatatg
    10741 cctaaatgga ccaataaaaa aggcctcttt tgcaagaggc cttgtagtgt ttagtacata
    10801 tccgacggcg gtgccgcagg cgttttcttc ttttcaggaa tctccgatac aatggcatcg
    10861 gtcgtcagca gcagagaggc aatcgatgcg ccgttctgca gggctgtacg ctccactttg
    10921 gtcggcacaa tcaccccgaa tttaatcata tcgccgtatt ccttgcggac cgcatcgtag
    10981 ccgaaattct tgtccgacga ctccattact ttctgagcga caatggaccc gtccaaaccg
    11041 gcattggccg caatctgctt aatcggtgcc acaaccgccc gccggacaat atccacacca
    11101 atctgctcat cgccggcgca tttaatcttg tccagtgccg gcaggcaccg cagcatcgat
    11161 accccgccgc ccggcaggat gccttcctcg acggctgcac ggcaggcatg cagagcatct
    11221 tccacccgtg ccttcttttc cttcatctcg gcctcggtgg ctgccccgac attaatctgg
    11281 gcaacgccgc cggccagctt ggccagccgc tcctgaagct tctcaatatc gtaatcgctg
    11341 gtcgaggcct cgatttcctt cttgatctgc tcgatgcggc ctttgatgtc gctggtcttg
    11401 ccggcgcctt cgacaatggt ggtcgtatcc ttatcaaccg tcacgcgctt ggcctgaccc
    11461 agctggctga gctcgacatt ttccagctgc agccccaagt cctcaaacaa tgcctgcccg
    11521 ccggtgagga tggcaatatc ctgcagcatt gctttgcggc gatcgccgaa gcccggcgcc
    11581 ttgacggcac aaacctgcag aacgccgcgc agcttgttga ccaccagcgt tgccagcgct
    11641 tcgccctcaa catcttcggc aataatcagc agcggacggc cctgcttggc aaccttttcc
    11701 agaatcggca ccaaagactt gaccgcgctg attttctttt catgaatcag aatatacggc
    11761 ttctcgagca ccacggtgcg ctcttctgga ttgttgatga agtgcgggct taaataccct
    11821 ttgtcaaact gcattccttc cagcagttcg acggtggttt ccaaagactg gccttcctcg
    11881 acggtaatca caccatcctt gccgaccttt tccatcgcct gtgccatcgt gcggccgatt
    11941 tcagcatcat ggttggccga gcaggttgcc acttgctcaa tctgcttggc cgaatccacc
    12001 ttgacgctca tcttctgcag ttcggcgacg atggcctcca ccgccttgtc gatgccccgc
    12061 ttgacctgca tggcattggc accggcggtg atgttcttga gaccttcctc aaaaatggct
    12121 tctgccagaa tggtagcggt ggttgttcca tcgccggcga cattgctggt cttggaagcc
    12181 acttccttga ccatctgggc gcccatattc tcatacgggt cttccagctc aatttctttg
    12241 gcgacagtaa cgccgtcttt ggtgaccgtc ggactgccga aggatttttc cagaatcaca
    12301 ttacgcccgc aggggcccag cgtaaccttg accgctgcgg ccagcttgcg gatgccttcg
    12361 cgtattgccg cacgggcttc ggtatcaaaa gcaatctttt ttgctgccat atttttaatc
    12421 tcctcttgtc agttgtcccg acttggcggg ttttgtctta ctcttcaata attgccagaa
    12481 tgtctgattc atccattatc agatattctt tgccgtctat ctttacttcc gttccggcgt
    12541 agctgccgaa cagaacttcc tgaccttttt tcacgctcat ttcagcccgc ttgccgtcgt
    12601 cgagaagttt gccttcgcct acggaaataa ctttgccccg ccggggcttt tctttggctg
    12661 tatccggaag cacaatcccg cctgccgtct tggcctctgc ttctaaacgc tggacaatca
    12721 ctttgtcccc taatggtctg attttcattg ctcgtctcct attgtctcaa aaaggtccat
    12781 aattaacaat tgataaaccg ctacgaaaga taattcttta gtgcaaacac tgtgccaggc
    12841 atataaaggc gcggtcttaa aatctataag ctcttttgca aaaataagat acgctgtaaa
    12901 cgataataac agcccaaatt tatctcaact caattcgaca gttcggcctt tcagatgcct
    12961 gccatgctgg cagattctta aaaagaccat ttgtgtctaa aaatagcgtc ttctttcatc
    13021 attggagata ttaggaagga aattgtttag ggcaaactga ataataggaa atattgtttt
    13081 gttaatcttg aaattgctgt tttccaaaga aaatgttgat tttaatagga aactttctaa
    13141 aatgacgagg attttttgtg cccataatgt tattagagca gcaaagttga taaatagata
    13201 agccggaaag gggaattttc cgtggtcaat actattttag aaagtactat agttgtactg
    13261 tccagtgtca ttttcgttct ttaactctca atggagccaa taactta
//
LOCUS       DAOTBO010000077        12954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4061_length_12954_cov_6.081634, whole genome shotgun sequence.
ACCESSION   DAOTBO010000077 DAOTBO010000000
VERSION     DAOTBO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4061_length_12954_cov_6.081634"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLE88_09160"
     CDS             <1..525
                     /locus_tag="PLE88_09160"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOM76670.1"
                     /translation="GLIYAKDLLTEIGKTPGQFNLRDKIRPAYFVPETKPLRDLLHEF
                     KTQKLHIAVVLDEYGGTAGIVTIEDILEELVGEIADEYEQATVQPYQKIDETTYEVDA
                     RMYIDDANSELEIELPDEEDYDTLGGFVFSHLGYIPKAGERFDYGNLTITVVSAGPRA
                     VKRLRIKKNFVVQQ"
     gene            528..1271
                     /gene="recO"
                     /locus_tag="PLE88_09165"
     CDS             528..1271
                     /gene="recO"
                     /locus_tag="PLE88_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOM76671.1"
                     /translation="MQIKDRAICLRAVDFSETSQVVTLLTRQHGKIAAMAKGSRRPKN
                     RFDGPIEIFSRGEAVFIAKESGGLAALTEFNQEPPFTAFAQNIEILYSALFGAELTEH
                     FLKEHDPHPELFDTFTAFLETLQKETSACRTLAWLILYQIRLLDKIGLAPVWDRCTNC
                     AAALPEKTAKKPSAVYFSSRNNGLLCVACEQAFVEKRAVQPAVVGVLAEPSKLPVTEI
                     HILQEIERLLIYHITELLGKAPKMAGHFT"
     gene            complement(1293..2057)
                     /locus_tag="PLE88_09170"
     CDS             complement(1293..2057)
                     /locus_tag="PLE88_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOM76672.1"
                     /translation="MSVKIRIMPCLDMQNGRVVKGVHFVNIRDAGDPVDCALAYCQAG
                     ADELAMLDITATVENRPTMLEVVRRVAEVVTVPFTVGGGIADVKSAEAVLNAGADKIS
                     TSSAAFRKPEMIKEMVKEFGPQRITVAIDVDKNASLPSGYEVYIDGGRTSTGKDAVEW
                     AKQVDEYGVPVILPTSKAGDGAKTGYDLPVIRAIKQAVAAEVVASGGAGRLEHFYEAV
                     EAGASILLAASVFHFNIISIKDLKSYLREKGVAVSE"
     gene            complement(2100..2894)
                     /gene="ccsA"
                     /locus_tag="PLE88_09175"
     CDS             complement(2100..2894)
                     /gene="ccsA"
                     /locus_tag="PLE88_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOM76673.1"
                     /translation="MMAIKTDLQGCLIYAVMLGYLAVLCLARRHPKQAKAVFLCSFLL
                     SAVAFIYRWAAVGHLPMQNMFEVFVFLGFCIWPIHQVSKKLLSVDYSVLLMIDAAVGL
                     IVLFPAGFVFHHKPQYLPPALQFWLFGPHVAAYMLAYIFMTKAAVFGLQGMRIPAGRP
                     ENAAIADYSYRLIAAGFPLLTLGLFLGSIWGKYAWGDWWGWDPKELWSLVCWLVYAIY
                     LHSRLVWPKRYSLHHVLAVVGLICIIITLLWVNLSKLFSGMHNYAS"
     gene            complement(2881..3921)
                     /locus_tag="PLE88_09180"
     CDS             complement(2881..3921)
                     /locus_tag="PLE88_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOM76674.1"
                     /translation="MRIKQLMNGLVLILLGILAVCAVYGAFLGAQQAQAFFSSPVMIG
                     FWCLLMAVLAAGFAVYSVLRRKSALMLIHGGCLAVLAGGMIGSQKAHQWAAARLGGPD
                     WTKGQMQLQPGQSASHVKLETKDQPGHLPFEVRLKDASIDYYDKPAIGLYFNDGAGWV
                     IKAEKGEPITIPDGRGTIEVSAVYRNFKLRSENGQMVPYESDEPGTNPAYQLVYRPAA
                     GQQETFYVFERFGMHTLSDRTCRAEYIAPRAVKDYKSTLQIIQNGRVVKEAAIEVNKP
                     LYYGGYHFYQHTFGYNQSEPVSGIMVVSARGIGFVFGGYGMILAGLAMHVWPKLFAQR
                     RKSDSRRGNDGN"
     gene            4095..5492
                     /gene="cobA"
                     /locus_tag="PLE88_09185"
     CDS             4095..5492
                     /gene="cobA"
                     /locus_tag="PLE88_09185"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOM76675.1"
                     /translation="MPKTQVYLIGAGPGEKDLITLKGLHILSTADVILYDHLIGPELL
                     ELAKPDAERISVGKCASSHTMPQEEINALIIQKAREGKVVARLKGGDCYLFGRGGEEA
                     EACFEAGLSFEVVPGITSALAAPCYAGIPPTHRDYASSVAIITGHRRKGDNRPIDIPH
                     ADTLIFLMSVGTIEAIIRSLREAGYPADTPFAAVEHGTCYNQRLIKGTLGNFPDVIKN
                     NPLRTPAVFIAGRVVQLQDKLDWFSQKPRILHLGTHPERCAHLGTIVHRPIIQCVEAE
                     DTELISRCLRGAGQYDWIVFTSAHGVKFFFKKLLTIGLDSRIFSKTRFAVIGRTTGQC
                     LLEFGIRADLCPADESSKGLLDEFVQIETDGLSILLPQADISSPELPEGLIKRGAKLQ
                     RLVIYQTIEKDIEDVDLNYIDKILFTSSSTVRAFVKKFGTVPKHIQALCLGIPTQITA
                     REFNIDAAIVNLNNP"
     gene            5516..6793
                     /gene="hemL"
                     /locus_tag="PLE88_09190"
     CDS             5516..6793
                     /gene="hemL"
                     /locus_tag="PLE88_09190"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOM76676.1"
                     /translation="MKNRRNALLYKKALKVIPGGVNSPVRAFKSVGGWPIFIQKAKGP
                     YLWDEDGSCYIDYCLSWGPLIFGHADKEILSEVKKTMVKGTSYGISTRLEIQLAEMIA
                     AAFDSIDKVRLVSSGTEAVMTAIRLARGFTGRDKIIKFIGCYHGHADPLLVSAGSGLT
                     TFGTPSSPGVPRECARNTLLAPYNDADAVERLLTKYAGRVAAVIVEPVAGNMGVILPK
                     TGFLQKLRTLCTKNSCLLIFDEVITGFRLCYGGAQTLYKVRPDLTTLGKIIGGGFPIG
                     AVGGKAEIMDMLSPQGAVYQAGTLSGNPVCVAAGIAALNKLKIQNPYSKLDLLTQSLC
                     SGISRILQAKGILHTINRIGSMFTLFFTEAEAADYDTACLSDTHRFAAYFRQMLSNGV
                     YLPPSQFEANFLSTAHADKHIHKTLEAVDKLVF"
     gene            6963..7268
                     /locus_tag="PLE88_09195"
     CDS             6963..7268
                     /locus_tag="PLE88_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76677.1"
                     /translation="MGWGRTFLLGDVGNRLDIADCEKDIRVLKESLMELHQEGQEIDS
                     ELLALQRENDRLKLYLAALVRLLINKNVLSRDEIEKMINIIDAEDGAIDGKARGKVL"
     gene            7448..7675
                     /gene="rpmE"
                     /locus_tag="PLE88_09200"
     CDS             7448..7675
                     /gene="rpmE"
                     /locus_tag="PLE88_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOM76678.1"
                     /translation="MKKDIHPKYDVVKVHCGCGNTFETRSTAKEIHAEICSLCHPFFT
                     GKQKFVDTAGRIDRFNKKFADGYSFQKKEKK"
     gene            7808..8896
                     /gene="prfA"
                     /locus_tag="PLE88_09205"
     CDS             7808..8896
                     /gene="prfA"
                     /locus_tag="PLE88_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOM76679.1"
                     /translation="MSDTNTAVLNKLAELDARCTDIEQQLSNPAVVSDVTRLVSLTKE
                     QGKLMPLVSRYREYKKLSAQLEEAQAILADPQQDAEMRELAESEAETLSAKQAVLLED
                     IKNTLVMADDASVDSVIMEIRPGTGGEEAALFARDLYQMYTRYADKQGWKIEVMDFSP
                     TEMGGIREVIMNIKGAGVWSKLGYEGGGHRVQRVPETESQGRIHTSAATVAILPEPEE
                     VDVQINPDDVIENVSCAGGPGGQNVNKVASAIRLEHIPTGIVVSMRDERSQHKNRAKA
                     YRILRSRVYEYYKSKADAERSSARKTMIGSGDRSQRIRTYNFPQNRLTDHRINLSLYS
                     LDKIMMGELDELIAALQDYDRQQRLQNL"
     gene            8915..10018
                     /locus_tag="PLE88_09210"
     CDS             8915..10018
                     /locus_tag="PLE88_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM76680.1"
                     /translation="MIIVVTGMVGIDKKQYLSEVCDYARQRGRSIHLYNVGSRMYEEA
                     PDIPAGRILDISLGRLHSLRRSVFKDIIAKSREYENVIVNTHATFRWRHGLFPAFDFY
                     QMRQLHPDLFICLIDGVEPLHLRLTREHPIHHTLKDLIVWREEEVLATQMMQQGTSEK
                     APCYILARGLTQPSVETFYRLAFEPRRKKAYLSFPMTHVAGLADVQTAIEAFRKSMKT
                     LLTCFDPADLEEAYLPLYAEQAQREGRHTVSVTVLGQAIELDAAEVRQIERDIRSQIY
                     ARDFALIDQSDMIVSFIPELPDGMPALSSGVERELQHAHEAAKQVYVIWMPKNKKPSI
                     FITQTANRVFRCPDEAMDYFETACGYKSDMLFE"
     gene            10132..11244
                     /locus_tag="PLE88_09215"
     CDS             10132..11244
                     /locus_tag="PLE88_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOM76681.1"
                     /translation="MKIKEIAQAIEEIAPLGLAQEWDNVGLLIGDPAKSIKTILLTID
                     TTKAVVQEAKTLKADLILAYHPIIWDGLKRITADGATANIYELIRAGISVFCIHTALD
                     TVAGGVNDALADILGMDNPKPIGDYVADPAGPQYKVITFVPDNAVNEVAEALYAAGAG
                     AIGSYSHCGFQTAGVGTFKPLTGAHPAIGKIGQIERVDEIRLETVVSAEKVSAVIAAL
                     RKSHPYETPAFDVFRHYNVENKLGLGRMGTLRTPLSIEQIITNVKKATGAAAAGIVGQ
                     PKRIIRTAAVCAGTCGKLLNTVIAAGCDLYLTGELKHHQALAAQEAGLTCICLSHTVS
                     ERFALKNLANRLKKRLKTCTIRISKKDKDPFIWKEI"
     gene            11244..12041
                     /gene="scpB"
                     /locus_tag="PLE88_09220"
     CDS             11244..12041
                     /gene="scpB"
                     /locus_tag="PLE88_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOM76682.1"
                     /translation="MQDETPDIANTQPDENLHGQASPCANGAEDEQLDRAIQQSETAQ
                     QSEADSQELEPALSKEHAADLPAPELTDTAEETDDEPEDVENPIEIEATLEAVIEAIL
                     FASDEPLSINRLAEIAEAGSVKDIRSAVTSLNKKYRTSNCSFRIEKISGGYQMMTLSA
                     FNPWISKLIKVRSDNKLTPAALETLAIVAYKQPIIRADIEAIRGVACGEVLRNLMYKG
                     LVKIVGRAEILGRPMLYGTTKKFLDAFGLNSLKDLPKVDELKNPEQG"
     gene            12072..12923
                     /locus_tag="PLE88_09225"
     CDS             12072..12923
                     /locus_tag="PLE88_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HOM76683.1"
                     /translation="MEEYDTPKFDEIGYWSEIKLEILSKYAPAYTSIMSKQSFVKGYY
                     YIDGFSGHGIHKSKTRDDFIQGSPSIALNTTPPFTEYFLIDLNSRKVEHLRQLAAGRS
                     NVHVYEGDCNEILLKKIFPVIKYEQYKRALCLLDPYGLHLNWDVIFTAGQSKAIEIFL
                     NFPVMDMNRNILWINPDKVDPLQANRMTSFWGDESWKDAAYRKIETLFGDNKEEKVPN
                     TNKKIAFAFRNRLKDVAGFGYVPEPIPMRNTCGATLYYLFFATPNKTANRIVEDIMDK
                     YRDRGVV"
BASE COUNT         3484 a         3306 c         3170 g         2994 t
ORIGIN      
        1 ggactaattt atgcaaagga tttgctgacc gaaatcggca aaacgcccgg ccagttcaat
       61 ctcagggata agattcggcc ggcgtacttc gtgccggaaa ccaaaccgct tcgcgacttg
      121 ctgcatgagt tcaaaaccca gaagctgcat attgccgtgg tattggacga atatggcgga
      181 accgcaggta ttgtaacaat cgaggacatc cttgaagagc tggtcggaga gattgccgat
      241 gaatatgaac aagccaccgt tcagccgtat caaaagattg acgagacgac atacgaggtc
      301 gacgcccgga tgtatattga cgacgccaac agcgaactgg aaatcgaact gccggacgag
      361 gaagactatg atacactcgg cggttttgtc ttcagccatc tgggctatat ccccaaggca
      421 ggggaacggt tcgattacgg taatctcaca attacagttg tcagtgcagg accgcgggct
      481 gtcaagcggc tgcgaatcaa gaaaaatttc gttgtccagc aataattatg caaatcaaag
      541 accgggccat ctgtctgcgt gccgtggact tttccgaaac ttcgcaggtt gtaaccctgc
      601 tgacccgcca gcatggcaaa atcgccgcaa tggcaaaagg ttcccgacgg cctaaaaatc
      661 gcttcgacgg tcccatcgaa atcttctcgc ggggagaagc ggtttttatt gccaaagagt
      721 ccggcggttt agcggccttg actgagttta atcaagaacc gccttttacg gcttttgcac
      781 aaaatattga gattctttat tcggccttgt tcggagccga gctgacagaa cattttctta
      841 aagaacacga cccgcatccc gaattatttg atacctttac agcatttctc gaaaccctcc
      901 aaaaagaaac ctctgcttgc cgaaccttgg catggctgat actttaccag attcgtctgc
      961 ttgacaaaat cggcttggcg ccggtgtggg atcgatgcac aaactgtgcc gctgccctgc
     1021 cggaaaaaac agcaaaaaaa ccgtctgctg tttatttttc cagtcgaaat aacggcctgc
     1081 tgtgtgtagc atgcgagcag gcgtttgtgg aaaaaagagc tgtccagccg gccgtggtcg
     1141 gtgtacttgc cgagccgtct aaacttcccg taacagaaat ccatatcctg caggaaattg
     1201 agcggctgct gatttatcac atcactgaac tgctcggcaa agccccaaag atggctggcc
     1261 attttacctg aacggccgga cagcagcact ccttactccg atacagcaac tcctttttct
     1321 cgaagataag atttcaggtc tttaatggaa attatattaa aatgaaacac ggaggcggcc
     1381 agcagaattg acgctccggc ctccactgct tcataaaagt gttccagcct gcctgcaccg
     1441 ccggaggcca caacttctgc ggcaacggcc tgttttatcg ctcgaataac cggcagatca
     1501 taaccggttt tggctccatc accggccttg ctggtcggca gaataaccgg cacgccatat
     1561 tcatctactt gtttggccca ctcgacagcg tcctttccgg tagacgttcg gccgccgtcg
     1621 atatacactt cataaccgct gggcagcgat gcatttttat caacatcaat ggccaccgta
     1681 atccgctggg gcccaaactc cttgaccatt tcttttatca tttcgggttt tcggaatgcc
     1741 gcactgctgg tggaaatctt gtcggctccg gcattcagta ccgcttcggc gcttttgaca
     1801 tcggcaatcc ccccgccgac agtaaacggc accgttacca cctctgcaac ccggcggacc
     1861 acctccagca tcgtcggccg gttttcaacg gtggctgtaa tatccagcat cgccaattca
     1921 tcggctcctg cctgacagta agccagcgca caatctaccg gatcacctgc atcccggata
     1981 ttgacaaaat gaacaccctt gacaacacgg ccgttctgca tatccagaca cggcataatg
     2041 cgaatcttaa cgctcatttt agtcctttcc aattttgctt ccatgctctc aacttaccat
     2101 caggaagcat agttatgcat tcctgaaaac aattttgaaa gattgaccca cagcagggta
     2161 atgatgatgc aaatcagacc gacgacagca aggacatggt gaagagaata tctcttcggc
     2221 cagaccaaac ggctatgcag gtaaattgca tacaccagcc agcagaccag cgaccaaagc
     2281 tccttcggat cccagcccca ccagtccccc caagcatatt tcccccagat ggagccgaga
     2341 aacagtccca gcgtcagcag aggaaatcct gccgcaatga ggcgatagct gtaatctgca
     2401 atcgctgcat tctctgggcg gccggcggga atccgcatcc cttgcaatcc gaacacggcc
     2461 gccttagtca taaaaatata agccagcata tacgccgcta catgcggccc gaacagccaa
     2521 aactgcaatg ccggcggcag atactggggt ttgtgatgaa acacaaatcc cgccggaaac
     2581 aggacaatca gccccacagc agcatctatc atcagcaaaa ctgaataatc taccgaaagt
     2641 aatttttttg aaacctgatg aatcggccag atgcagaagc ccagaaaaac aaaaacctca
     2701 aacatattct gcatcggcag atgcccgaca gcagcccatc ggtaaataaa cgctactgca
     2761 ctcagcaaaa atgagcacaa aaagaccgcc tttgcctgtt ttggatgccg ccgtgccagg
     2821 cataaaacag ccagatatcc cagcatcacc gcatatatca gacagccctg caaatccgtc
     2881 ttaattgcca tcattccccc gtcggctgtc cgattttctt ctttgtgcaa aaagcttggg
     2941 ccacacatgc attgccagcc ctgccaaaat cattccgtag cccccgaata caaatcctat
     3001 cccccgtgcc gacaccacca taatcccgct gaccggctcg gattgattgt atccaaacgt
     3061 atgctgataa aaatgatatc cgccgtaata gagcggttta ttgacctcaa tagcggcttc
     3121 ttttacaacc cgcccattct gtataatctg cagggtactt ttgtaatcct tgacagcgcg
     3181 gggagcaata tattctgccc ggcacgttct gtcggacagc gtatgcattc caaagcgttc
     3241 aaacacataa aatgtctcct gctggcccgc tgccggccgg tatacaagct gataggccgg
     3301 attggttccc ggctcatccg attcataagg caccatttgt ccgttttcgc ttctcagctt
     3361 aaaattccta taaaccgcac tgacctcaat tgttccgcgg ccgtccggta ttgttatggg
     3421 ctcccctttt tccgctttaa tcacccatcc cgcgccgtca ttaaaataca gtccaatcgc
     3481 cggtttgtcg taatagtcaa tcgatgcatc tttaagccgc acctcaaagg gcagatgccc
     3541 cggctggtct ttggtttcca gtttcacatg actggcggat tgccccggtt ggagctgcat
     3601 ttgtcctttg gtccagtccg gaccgcccag acgggcggct gcccattgat gtgctttctg
     3661 cgaaccgatc atcccgccgg caagcacagc caaacagccc ccgtgaatca gcataagagc
     3721 cgattttcgc cgcaggaccg aatacaccgc aaaacctgcc gctaatacag ccatcagaag
     3781 gcaccagaat ccaatcatta caggagaact aaaaaatgcc tgggcctgct gtgctcccag
     3841 aaaagcccca taaacggcac agactgccaa tatccccagc agtatcagga caagtccatt
     3901 catcagctgt ttgatgcgca tcaatatgat tcttataaga atgccgtgcc gccgcttttc
     3961 ggcagctgct ttttttattg actgttatcg gccgccgtac tatacataga ggcaattata
     4021 cagagtccgg ctgaaaacaa aagaccaaaa actgccggac aaatgcattt gacagaaacc
     4081 agagagtctt tgacatgccg aaaacacagg tttacctcat cggcgctggt cccggcgaaa
     4141 aggacttgat aaccctcaaa gggctgcaca tcctgtcgac agcggatgtt attctctatg
     4201 accacctgat tggcccagag cttctcgaac tggccaaacc agatgcggaa agaatttctg
     4261 tcggcaaatg cgcttccagc catacaatgc cgcaggagga aatcaacgcc ctcattattc
     4321 aaaaagcccg tgaaggcaaa gttgttgccc gtctcaaggg cggtgattgc taccttttcg
     4381 gcagaggcgg cgaagaagcc gaagcctgtt ttgaagcggg tttgtctttt gaggttgtcc
     4441 cgggaatcac cagtgccttg gcagccccct gctatgcagg cattcctccg acccaccggg
     4501 actatgcatc cagcgtcgct atcatcaccg ggcaccgcag aaaaggcgat aaccgcccca
     4561 ttgatatacc ccacgccgat acgctgattt ttctgatgtc ggtcggcact attgaagcta
     4621 tcatccgctc gcttcgggag gcgggctatc cggcggatac accctttgcc gctgtcgagc
     4681 atggcacctg ctacaaccag cggctcatca aagggaccct cggcaatttt cccgatgtca
     4741 ttaaaaacaa tcccctgcgg acgccggcgg tattcattgc cggcagggtt gtccagcttc
     4801 aggacaaact cgactggttt tcccaaaaac cccgcattct ccatctgggc acacatcccg
     4861 aacggtgcgc ccatctgggc acaattgtcc accgtccgat tattcagtgc gttgaagccg
     4921 aggataccga gctaatcagc cgctgtctgc gcggggctgg tcaatacgac tggattgtct
     4981 ttaccagtgc tcacggggtc aaattctttt ttaagaagct cttaaccatc gggcttgaca
     5041 gccgcatctt ttccaagact cgctttgctg ttatcggccg cacaaccggt cagtgcctgc
     5101 ttgaattcgg catccgagcc gacctctgcc ctgctgatga atccagcaaa ggcctgctgg
     5161 atgaatttgt acaaatagag acggacgggc tttccattct gctgccgcag gccgacatca
     5221 gttcgcccga actgcccgaa ggtttgatca aaagaggggc aaaattgcag cggctggtca
     5281 tctatcagac tattgagaag gatattgagg atgtcgattt gaattacatc gacaagattc
     5341 tttttaccag cagctcgacg gtccgcgcct ttgtcaaaaa attcggtacc gtgcccaaac
     5401 atattcaggc attgtgtctg ggcattccta cccaaataac tgcaagagaa tttaatattg
     5461 atgcagcaat agtaaacctg aataatccat aataattctg ggaaaattta gaaatatgaa
     5521 aaaccgcaga aatgccctgt tgtataaaaa agccctgaaa gtgattcccg ggggcgtcaa
     5581 cagccctgtc cgtgctttca aaagcgtcgg cggctggccg atattcattc aaaaagccaa
     5641 aggcccctat ctatgggatg aggacggcag ctgctacatt gactattgcc tgtcgtgggg
     5701 acccttgatt tttggccatg ccgataaaga aatattgtcc gaagtaaaaa aaacaatggt
     5761 taagggcacc agttacggca tctcgacgcg gcttgaaatc cagctggcgg aaatgattgc
     5821 tgcggctttc gactccatcg acaaggttcg gctggtcagt tccggcaccg aagccgttat
     5881 gaccgctatc cgtctggcac gcggctttac aggacgggat aagattatca aattcatcgg
     5941 ctgctaccac ggccatgccg atcctcttct ggtcagtgcc ggctcgggat tgacgacctt
     6001 cggcactcct tccagccccg gtgtgcctcg ggaatgtgcc agaaacacgc tgctggcacc
     6061 ctacaatgat gccgatgcag tcgagcggct tctgacaaag tacgccggca gagtcgctgc
     6121 agtaatcgtt gagccggtgg ccggcaatat gggagtcatt ctgcccaaaa ccggatttct
     6181 gcagaaactg cggacactgt gcacaaaaaa cagctgcctg ctcatctttg atgaggtcat
     6241 cacgggcttt cggctctgct acggcggcgc acaaaccttg tacaaagtcc ggcccgacct
     6301 gaccacatta ggaaaaatta tcggcggcgg ctttcctatt ggagctgtgg gcgggaaagc
     6361 agagattatg gatatgctct ctccgcaggg tgctgtttat caggcaggca cactttccgg
     6421 aaatcctgtc tgcgttgccg ccggcattgc tgcactcaac aaactcaaaa tacaaaatcc
     6481 gtacagcaag ctggacttgc tgacccaatc tctctgcagc ggcatcagcc gcatactcca
     6541 agctaaaggt attcttcata ccataaaccg catcggctcc atgtttacgc tttttttcac
     6601 cgaggccgag gcagcggatt atgatacagc atgtctttcg gacacgcacc gcttcgctgc
     6661 atacttccgg cagatgcttt ctaacggtgt ctatctgccg ccatctcaat ttgaagcaaa
     6721 cttcttatct accgcccatg ctgacaaaca cattcataag actttggagg ccgtggacaa
     6781 gctcgtcttt taaggctgac caacaataac taagatgaga gggcacttgc cgacactaaa
     6841 atcctgcggg cattcaggac tgaacagatc tgatgataaa atttatgcgt tgaaaatcct
     6901 gcaataattg gataaattga tttttgccga cacaaacctt ttcttatgaa aggaacatgc
     6961 aaatgggctg gggacggaca tttttactgg gggatgtagg caatcggctg gatattgccg
     7021 attgcgaaaa agatattcgg gtacttaaag agtcgctgat ggagctgcat caggaaggac
     7081 aggagattga ttcagaactg ttggcgcttc agcgagaaaa tgaccggctc aaactgtatt
     7141 tggctgccct tgtgcgtctg cttataaata aaaatgtact cagccgggat gaaatcgaga
     7201 aaatgatcaa tattattgac gctgaagatg gggcaatcga cggaaaagcc cggggcaagg
     7261 tcttgtaaca attcaccaaa gacttaataa gctgacagat ataaaagcgg cttgcttcag
     7321 agtagaagat atttcttctc tttatatacc aaaataacat ctgcaaaaaa ccaaaaaaaa
     7381 gattgcattt ttattttcat ttggtaaact tctttctttt aattttagga tatttggaga
     7441 gtttataatg aaaaaagata ttcatccaaa atacgatgtt gtaaaggttc attgcggctg
     7501 cgggaatacc tttgaaacac gcagtacggc caaggaaatc cacgcagaaa tctgctccct
     7561 gtgccatccg ttttttacag gcaagcaaaa gtttgtggat acagccggcc gtatagaccg
     7621 gtttaacaag aaattcgctg acgggtattc cttccagaaa aaagaaaaga agtagctgag
     7681 tttgtactta caccattgcg ctgttaagct taatcgaaca gattaagtat tggcagcgta
     7741 atttttttgc cttcagaaga atacgactat tatagtcgta ttttttggaa aaaaccggga
     7801 gttatatatg tctgacacca ataccgctgt tttgaacaag ctggccgagc tggacgccag
     7861 atgtacggat atcgagcagc agctcagcaa tccggccgtg gtttcagatg tcacccgatt
     7921 ggtttccctg acaaaagaac agggcaaact gatgccgctg gtaagccggt atcgcgagta
     7981 taagaagctt tcggctcaac tggaagaagc acaggcaatt ttggcagacc cgcagcagga
     8041 cgccgagatg cgggagcttg ccgaatcgga agctgaaaca ttatctgcca agcaggcagt
     8101 cctgctggaa gatattaaaa atacgctggt gatggcagat gatgcttcgg ttgattcggt
     8161 catcatggaa attcgtcccg gtacaggggg ggaagaagcg gccctgtttg cgcgtgattt
     8221 gtaccaaatg tacacccgct atgcggataa acagggctgg aagattgagg tgatggattt
     8281 ttctccgacg gagatgggag gcattcgaga agtcattatg aatatcaagg gagcaggcgt
     8341 gtggtcgaaa ctgggatatg aaggcggggg acatcgcgtc cagcgtgtgc cggaaacaga
     8401 atcgcagggc cggattcaca cctcggctgc cacagtggcc attctgcccg agccggagga
     8461 agtcgatgta cagattaatc cggatgatgt gatagaaaat gtcagctgtg ccggcggtcc
     8521 cggggggcag aatgtcaaca aggttgccag cgccatccgg ctggagcata tcccgacagg
     8581 gattgtcgtc agcatgcggg atgagcgcag ccagcacaag aaccgggcaa aggcctaccg
     8641 cattttgcgc agccgggtgt atgagtatta caaatcaaaa gccgatgctg aacggtccag
     8701 cgcccgcaag acaatgatcg gctcggggga ccgttcgcag cgaatccgga cgtataattt
     8761 cccgcagaac cgcctgaccg atcaccgcat caatttgtcg ctgtacagct tagataagat
     8821 tatgatgggc gaactggatg agctgattgc cgcattgcag gactacgaca gacagcagcg
     8881 tctgcagaac ctataataaa aggatgtctc agcgatgatt attgtcgtta caggcatggt
     8941 gggtatcgac aagaagcaat acttgtccga agtctgcgac tatgcccggc agcgcggcag
     9001 aagcatccat ctgtacaatg taggcagccg aatgtatgaa gaagccccgg atatacccgc
     9061 cggaagaata ctggatatct cgctgggtcg gcttcattca ctgcggcgaa gcgtttttaa
     9121 agatattatt gccaaaagcc gcgaatacga aaacgtcatt gtcaacaccc atgcgacatt
     9181 tcgctggcgg cacggcctgt ttccggcgtt tgatttttat cagatgcgcc agctccatcc
     9241 cgatttgttc atctgcctga ttgacggtgt tgagccgctg catctgcggc tgacacggga
     9301 gcatccgata caccataccc tcaaagattt gattgtatgg cgggaggaag aggtgctggc
     9361 cacccagatg atgcagcagg gaaccagcga gaaagccccc tgctatatct tggccagagg
     9421 tcttacccag ccttctgttg aaacctttta ccggctggcg tttgagccgc gccgcaaaaa
     9481 agcttatttg agctttccga tgacccatgt cgccggcctt gcggatgtgc agacggcgat
     9541 tgaggcgttt cggaagtcca tgaaaacact gcttacctgt ttcgatccgg ccgacttgga
     9601 agaagcctac cttcctctct acgccgaaca ggcacagaga gagggcagac acacagttag
     9661 cgttactgtc ttgggacagg ctattgaact ggatgcggcg gaggttcggc agatcgagcg
     9721 ggatatccgc agtcagattt atgcgcggga ttttgcattg attgaccagt cggatatgat
     9781 tgtcagtttt atccccgaac tgccggacgg catgccggcg ttgtccagcg gggttgagcg
     9841 cgaactgcag cacgcccacg aagcagccaa acaggtctat gtcatctgga tgccgaagaa
     9901 taaaaagcca tcgatcttta tcacccagac agccaatcgc gtatttcgct gccctgatga
     9961 ggcgatggat tatttcgaaa ccgcctgcgg ctataaaagc gacatgctgt ttgagtagaa
    10021 gccataaatg tccagaaggg atgagatata gcagccggag atgaaatctc cggcaaagga
    10081 gctataatag gaattaagcc ccaaaggggc caaacaaaat ggaacattaa aatgaagatt
    10141 aaagagattg cacaggcgat tgaggagatt gccccgctgg ggctggcgca ggagtgggat
    10201 aatgtgggtc tgcttatcgg cgatccggcg aaatccatca aaaccattct gctgaccatc
    10261 gacacaacaa aagcagtcgt acaagaggcc aaaacactga aagcggattt gattttggcg
    10321 tatcatccga ttatctggga cggcctgaag cgcattaccg ccgacggagc taccgctaat
    10381 atttatgaac tgattcgagc gggcatcagc gtcttctgca ttcataccgc gctggacacg
    10441 gtcgccggcg gagtcaatga cgctttagcc gacatactcg gtatggataa tccaaagccc
    10501 atcggcgact atgtcgccga tccggccggg ccgcaatata aggtgattac atttgtgcct
    10561 gataatgccg taaatgaagt agcagaagcc ctgtatgccg ccggtgcagg ggctatcggc
    10621 agctacagcc attgcggctt tcagaccgca ggtgttggaa catttaagcc attgacagga
    10681 gcacatccag ccatcggcaa aatcggacag atcgaaagag tggacgaaat tcgtctggaa
    10741 acggttgttt ctgccgagaa agtttcagca gtaattgccg cccttcgaaa aagccacccg
    10801 tacgagacac cagcgtttga tgtattcaga cattataatg tcgaaaataa gctcggatta
    10861 ggccgaatgg gtacccttcg aactcccctt tcgattgaac aaatcatcac aaatgttaaa
    10921 aaagctactg gcgcagcagc cgccgggatt gtaggacagc caaaacgtat cattcggacg
    10981 gcagccgtct gtgcgggaac ctgcggcaag ctgctcaaca cggtcattgc cgccggctgc
    11041 gatctgtatc tgaccggcga actcaaacac catcaggcac tggccgcaca ggaggcggga
    11101 ttgacctgca tctgcctgtc gcatacggtt tcggagcggt ttgcgctgaa aaatctggca
    11161 aatcgcttga aaaaacggct caaaacctgc acaatacgga tcagtaaaaa agacaaagac
    11221 ccctttatct ggaaagagat atagtgcagg acgaaacccc tgatattgcg aatacgcagc
    11281 cggatgaaaa cctccacggc caggcatccc cctgcgccaa cggtgcagag gatgagcagc
    11341 ttgaccgggc tatccaacaa tccgaaacag ctcagcagtc cgaagcagac tcgcaggaat
    11401 tggagcctgc cctgtccaaa gagcacgcag ccgatctgcc agctccggaa ttgacagata
    11461 ctgccgagga gacggatgac gagccggagg atgtggaaaa tccgattgag attgaggcca
    11521 cacttgaggc agtaattgag gcgattttgt ttgcaagcga cgagccgctc agcatcaatc
    11581 gtctggcaga aattgctgag gccggttcgg taaaggatat ccgctcggct gtcacgtcgc
    11641 ttaataaaaa ataccgcacc agcaattgct cgttccgcat cgaaaaaatc tccggcggct
    11701 accagatgat gaccctctcg gcctttaatc cgtggatcag caagctgatt aaggtgcgtt
    11761 cggacaataa gcttacgccg gctgccttgg aaacgctggc gattgtggct tataagcagc
    11821 cgattatccg cgctgatatc gaagccatcc ggggcgtcgc ctgcggcgaa gtgctgcgca
    11881 acctgatgta caaagggctt gtcaaaattg tcgggcgggc tgaaatctta ggccgtccca
    11941 tgctgtacgg gacaaccaaa aagttccttg atgccttcgg ccttaactcc ctcaaagacc
    12001 tgcctaaggt cgatgaactt aaaaatcctg aacaggggta acaatggtaa ctatccaaga
    12061 aggagcgtat tatggaagag tacgacactc caaaatttga cgaaattggt tattggtcag
    12121 agattaaatt ggaaattttg agcaaatatg cacccgcata cacttctata atgtccaaac
    12181 agtctttcgt caaagggtat tattatattg acggattttc tggccatgga attcataagt
    12241 caaaaacacg cgatgatttc atacagggaa gtccttccat tgcactcaac accacaccac
    12301 cctttacgga gtattttctg atcgatctta atagcagaaa agtcgagcat ttacgacagt
    12361 tagccgccgg tcgatccaat gtccatgtct atgaaggaga ttgtaatgaa attctgctta
    12421 aaaagatttt ccccgtcatt aaatatgaac agtacaaacg ggctttatgc ttgttggatc
    12481 cttatggact tcatttgaat tgggatgtaa tctttactgc cggacagtca aaagcaattg
    12541 agatattcct taattttcca gttatggata tgaataggaa tatattgtgg attaatccag
    12601 ataaggttga tcctctccaa gcaaacagaa tgacatcctt ttggggagat gaaagctgga
    12661 aagatgccgc atacagaaaa atcgaaactc tattcggcga caataaagaa gaaaaagtac
    12721 ccaacactaa taaaaaaata gcatttgctt ttcgcaatag attaaaagat gttgccggtt
    12781 ttgggtatgt acctgaaccg ataccgatga gaaatacatg cggagccacg ctttattatc
    12841 ttttctttgc aactccaaat aaaacagcca atcggatagt agaagacata atggataaat
    12901 acagagacag gggagtcgtc tgatggcaac tcaaacggct attgaatgga cgga
//
LOCUS       DAOTBO010000078        12915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4077_length_12915_cov_5.868274, whole genome shotgun sequence.
ACCESSION   DAOTBO010000078 DAOTBO010000000
VERSION     DAOTBO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4077_length_12915_cov_5.868274"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..995)
                     /locus_tag="PLE88_09230"
     CDS             complement(18..995)
                     /locus_tag="PLE88_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOM76684.1"
                     /translation="MNILLTSVGRRGYLVRYFKDALGPEGQVWGADSSVFAPAFTYCD
                     GTAILPEVKKDHYPKALLDLCKTRKIDIVVPLIDPELEILGGWQQQFENEGVFLLLSP
                     VETIAMASDKYLTYQFAVKNGLRTPCSFLTVEEAMESLDAGKLSWPLLVKPRKGSASI
                     DIVWCYDKLQLEAAMESVREPMIQELVKGVEYGYDLFGDRQGRPISVYCKRKLAMRAG
                     ETDKAITVNEPALMEFGQKLLSAMKLVGPADVDAIADKDGIKLLEINPRFGGGYPCSH
                     LAGADFCKKIIAMRNGCNLTADIGSCPAGVCMLKQDEIICPSWPIQPNG"
     gene            1273..2463
                     /locus_tag="PLE88_09235"
     CDS             1273..2463
                     /locus_tag="PLE88_09235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOM76685.1"
                     /translation="MIFSEFVGPERPLSWSLQFWPVGVCAFIVALAATWLCKKIALKL
                     GIVDRPDHRVKTHKEPVAYLGGVGILAGLTVGILAGIVLIKDFEVFDKCLKWLLGILA
                     GAGTACFVGLVDDIMDLKPKQKFLGQIIAACALLATGIRPDLQHAAAFLGWNMPDSIE
                     FLFGVFIVVFFVLGATNSLNLLDGLDGLCGGVTAIITVAFLLLSIHRATWGFSEASDP
                     VRIIVCLGLVGGVLGFLPFNRHPAKIFMGDAGSMLLGFATASLMILFAEEIFRWWMAS
                     IVIFGLPILDTATALVRRWLNKKPLFVSDRGHIYDQMMDRGIPLKKTVAICYGLAGMY
                     AFIGLAISQIRTRYAIFVYTAVIAASALIIWKKGFLKMEGYRGAIRKSEDSEIKNQQI
                     QSET"
     gene            complement(2484..3716)
                     /locus_tag="PLE88_09240"
     CDS             complement(2484..3716)
                     /locus_tag="PLE88_09240"
                     /inference="COORDINATES: protein motif:HMM:NF004936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOM76686.1"
                     /translation="MSEQKVYILGGYQTDFARNWARENKHISAMMREAYEGSLMTTKI
                     EPKDIKAAFIGNFAAELYCMQGHLGAFFVDFDPAFRGLPAMRFEGACASSALAALSAM
                     AHIQAGLYDCIALMGVELMKSVPAAKGGDYLGTAAWYERESAGVEFPFPKLFGKLGDV
                     YEARFGLDDDYLARIAAINYANAKRNPNAQTRDWFMSYEHACTRGQYNPSVSGRIKVT
                     DCSQITDGAVCLYLASKSFAEQYARRCGISPDALPYIAGWGHTTAPIEFAAKIAESEN
                     QSYVLPWTRRAITDAYARAGIADCWDLDAIETHDCFTTSEYMAIDHFGLTPPGQSWQA
                     IEDGVIELSGKLPINPSGGLIGCGHPVGATGARQLLDAYKQVTGTAGDYQVKDASRVA
                     TLNIGGTATTNVCFIVEK"
     gene            complement(3760..4014)
                     /locus_tag="PLE88_09245"
     CDS             complement(3760..4014)
                     /locus_tag="PLE88_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOM76687.1"
                     /translation="MSSCAVSLFAQGHLLRANLEEANAAPSRADFKCLKEAWETYDWL
                     RLYQSTGIVTELHLKELLKESNEIISILTTIVKESKLKKD"
     gene            complement(4088..5371)
                     /locus_tag="PLE88_09250"
     CDS             complement(4088..5371)
                     /locus_tag="PLE88_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOM76688.1"
                     /translation="MKIETVLILGASGTIGSLTGGLLAQNGIKIFFLSRTSAGSLKGL
                     ARAQKQARSETIAKHITCGDYEGLLPDACRQADWIIECVAEDYSIKQGLYERVDNLRR
                     PDAIVSSVTSSLLLDELPKGRSASFRRHFLSTHFYNPPGKMTACEICGTSETDPAVVD
                     FISDFCSSKLRRAVIRVKPTAGFAGNRLAFVLFARITELAAEFSVEMMDYLIGPYTGR
                     LMPPLATLDLVGLDIHKAIIHSLAAHTDDAFHHKLVLPAYIEKMIETGHLGNKTRDKG
                     GFYKKLESGDFMYIDPATGSYIPSFHPHVRFVETAKDQIHLAKYRQAFDTIVAAKGTE
                     ADIVREILATYIAYGYMLAGEVTDAEYGITGIDRVMSTGFNWASPSLMVYMLGGKDRA
                     MELIKMQKLPVPELLRTDDTCEHYIFNAGKYFPAK"
     gene            complement(5457..6029)
                     /locus_tag="PLE88_09255"
     CDS             complement(5457..6029)
                     /locus_tag="PLE88_09255"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOM76689.1"
                     /translation="MKVDNIITADHVRLRPLTEQDMTLKVKWYNDPLVRKTLVIDEQF
                     ELDKTIEWFQSLAGRNDRADFAVESLDGVPIGITGLIDIDRRNKTAQCYCVIGEKTFW
                     GQGLGTEIHSVLFQWAFEKLDIQKIWAHIRTNNPAIFRVVEKLGFQIEGTLRRHAVVN
                     SQRIDLYQIGLLREEFIPTHQHYEMLDRPQ"
     gene            complement(6134..7072)
                     /locus_tag="PLE88_09260"
     CDS             complement(6134..7072)
                     /locus_tag="PLE88_09260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOM76690.1"
                     /translation="MQKFLVTGGAGFIGSNICRKLVADGCFVRVIDNLITGKKSNLAD
                     IIDKIDFIEADMGDLDIAKRAMKDIDAVLHQGAVPSVPKSVADPATTHHHCVDATFNL
                     LLAARDMKIKRFVYAASSSAYGDSEVLPKVETMPVAPKSPYAAAKLFGEYYCSVFAQC
                     YGLETISLRYFNVFGPYQDPTSQYAAAIPAFVTHILKGQPPVIYGDGEQTRDFTYVDN
                     IVHANLLAARAKKTSGQVINVACGERISVNAIIDLINRICGTNVRPVYADPRPGDVKH
                     SLAAIDRARELIGFQPVVLFQEGLTKAIDWYRKNLL"
     gene            complement(7125..8372)
                     /locus_tag="PLE88_09265"
     CDS             complement(7125..8372)
                     /locus_tag="PLE88_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76691.1"
                     /translation="MLSRSDILKMESIACGTPFYVVSRQQFESNFKDLTAAFSSRYQP
                     FILAYSYKTNYLPYLCGIIKEKGGWAEVVSRLEYDLAVKIGHDPQKIIFNGPVKTAED
                     IVQAVEKGSIVNADSPSELQLITDYAKADPRRPVRVGLRINIALSDAAGQSHIQNALK
                     TGRFGLDPAEFKSKLNIQDLKQLPNLQIVSLHGHTSTADRSRWCFEVITETLCRLAEE
                     FFPDSVEYLNIGGGFFGRMRPDMPFTDVPGFDDYAETICSVLGRNSWVRKKTPALVIE
                     PGVAMAADAVRFITKVVSVKSIRGDLFITVDGSAFHIKPTFHALNLPHQVITRNADPP
                     IHTYSVVGATCMEKDILLRNITASLPEPGDYICIDNVGAYTLVLTPPFITPAPAVLAD
                     EDGCFKLVRRRQTLDDIFSSYVF"
     gene            complement(8382..8999)
                     /locus_tag="PLE88_09270"
     CDS             complement(8382..8999)
                     /locus_tag="PLE88_09270"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOM76692.1"
                     /translation="MVKRFFDIVVSLVLLVLLSPLILITALLVRLKLGSPILFCQQRP
                     GLHGKLFTIYKFRTMISRTHDEQGNILPDEKRLTPFGLWLRGTSLDELPELLNVLKGD
                     MSLVGPRPLMAKYLDRYTPQQNRRHEVKPGITGWAQVNGRNDMTWEQKFQYDVWYVDN
                     RSFWLDLKILWLTLWHVISRKGVAKEGYATVDEFMGTKADNTKSE"
     gene            complement(9093..10151)
                     /locus_tag="PLE88_09275"
     CDS             complement(9093..10151)
                     /locus_tag="PLE88_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOM76693.1"
                     /translation="MKKQKSPVKLFAGVFAILIVVTILRHLLRPKSVQFSLDIRDNAG
                     VALLETVGDSLVCVFSDGRTCIWDWNNLPSPKGDFRAVSCLAAVLDASRLAAVPEKGK
                     KILTVYDLPAGRRQKELSVGWEDQSVFLRISPDKKVLLLMRKNPADSAGKILYEFAKL
                     DIENELIGPAVTISISEKTDKIVDFAASSNQVIYAVGSKSQAGWVGAVDLHQGKIVQD
                     IVYADTKEFCSAAVSPDGQYLLAGDRDGILYKIDAADLNILKKVNLLAPGETRPVTND
                     YSVLNLAFSNDGRYYVATINPKAYLLQTGSDTVFHWCTPTSNLTSKIAYSPDSRFFAT
                     SDIRASKPIRIWETPVSK"
     gene            complement(10208..11350)
                     /locus_tag="PLE88_09280"
     CDS             complement(10208..11350)
                     /locus_tag="PLE88_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOM76694.1"
                     /translation="MDKFEQSFSAWLNCPYAFSFWKARVALYAILRGLNIGDGDEVIL
                     PGYTCVMDVNPIKYVGAKPVYADIEPNTFNMVPDFVEEKITLRTKLIIAQHTYGYPCD
                     MERILQIAQKHAIPVIEDCCLALGSRYKGQLVGTFGRAAYFSFQWNKPFTTGLGGMAI
                     TNDSGLAQKIERLYAAEATEPSAKDVLMLAAQLAVYRAFIYPRTTALAQTLFRFLTRK
                     GLVVGSSSSAEFVPSMPSGFFKGMSAVQARSGIKQLNKLEDNICHRRKMADLYDHLLS
                     EKGWPIRCYDKSVMDPVLVRYPVRIKQKQQALETAAAAGIELGSWFESPLHPQETPLA
                     AYDYTLGMCPEAEKAAAETVNLPLHPRAGEKTVRRTADFISQFTPA"
     gene            complement(11389..12009)
                     /locus_tag="PLE88_09285"
     CDS             complement(11389..12009)
                     /locus_tag="PLE88_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM76695.1"
                     /translation="MNYKIIPLQKSHIDLVVDIHIKAFPGFFLSFLGPRFLKEFYQSF
                     AEDPIGIGFVAVDSDCNQVIAVTVGPLVPAGYFKRLLKRKWWAFCLASISAVIKKPSV
                     IKRLFRAVFYRGDSPAHADALALLSSIAVSPDVQAKGIGAALVNAFVQEVKKRGGKGI
                     FLTTDAQGNDKVNRFYQKMGFTLESSYTTPEGRQMNRYILIFSNHN"
     gene            complement(12165..>12915)
                     /locus_tag="PLE88_09290"
     CDS             complement(12165..>12915)
                     /locus_tag="PLE88_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM76696.1"
                     /translation="ITIAPGMKQRLIERGVPENKIEVVYNWCDQAQICPAEKNQELAK
                     SLGMAGRFNVVFAGNMGKAQALEPVLDAAKIVADRQPQVQFVFIGSGVCVEELKQKAA
                     DMGLTNVLFLPRRPVSEIGDILNLSDVLFVHLKKDPLFKITIPSKIQSYLAAGRPILV
                     AVPGDASALVQEAQAGLACEPENPQSIADAVCQFSVMSKSELEAMGHRGKQFYQQYLS
                     FEIAAAKIEHILLSQVSRRPNASQQSLAANY"
BASE COUNT         3384 a         3151 c         3094 g         3286 t
ORIGIN      
        1 ggggcagaga tacaaattca accattcggc tgaataggcc aagagggaca gataatctca
       61 tcctgcttaa gcatacacac tccagccgga caggagccaa tatcagctgt cagattgcat
      121 ccattccgca tcgcaataat ctttttgcag aaatccgccc ctgccaaatg cgagcaggga
      181 tagccgcctc cgaagcgggg atttatttca agaagcttga tgccgtcctt atctgctatc
      241 gcatccacat cagcaggtcc taccagtttc atagcagaca gcagtttttg cccaaattcc
      301 atcaaagccg gctcattaac agtaatggcc ttgtcggttt cgcctgcccg cattgccaac
      361 ttccttttac aatagacgct gataggacgc ccctgccgat cgccgaaaag gtcatagccg
      421 tattcgacac ccttaacaag ttcctgtatc atcggttccc gaacagattc catcgccgcc
      481 tcgagctgaa gcttgtcata gcaccatacg atatcaatag aagcgctgcc cttgcgcggc
      541 ttgaccagca gcggccagct gagcttgccg gcatccaatg attccatggc ttcctcaaca
      601 gtcagaaagg agcacggtgt gcgcaaaccg tttttaacag caaattgata agtaaggtac
      661 ttatcggaag ccattgcaat cgtttcaacc ggagaaagca gcaaaaaaac gccttcgttt
      721 tcaaactgct gctgccagcc gcctaatatt tcgagttccg gatctattag agggacaaca
      781 atatcgattt ttcttgtttt gcacagatct aatagtgcct ttggataatg atcctttttg
      841 acttcgggaa ggatagctgt tccatcacaa tatgtaaaag ccggcgcaaa aacgctgctg
      901 tcagctcccc agacctgacc ttctggacca agagcatcct taaaatacct gacaaggtac
      961 cctcgccggc caacggatgt tagcaaaata ttcataaaat cggcactgtc aactccggaa
     1021 ttactgtttt ccagcggatt ataataaacc gactgtatta ttcagtcaat ttattttcgg
     1081 cattttttgt cttttaataa agcagaaaca ttatataatg ttcttgattt tttatatggt
     1141 gtaatatctg gcaaaatgct ccatcataag gctcgatgca tatttccctt ttcgggaatg
     1201 ggggactaat aagtcctcat tccagattcg aaggcaagat aaagaccaaa cacggtatct
     1261 gcgggaggtt atatgatatt cagtgagttt gtaggaccag agagaccgct aagctggtcg
     1321 cttcagtttt ggccggtagg cgtgtgcgcc tttatcgtgg cactggcggc aacatggctg
     1381 tgcaagaaga tcgctctgaa gctggggatt gtagacagac ctgaccatcg tgttaaaaca
     1441 cacaaagaac cggtggccta tttggggggt gtggggatac ttgcggggct gacggtggga
     1501 atactggccg ggattgtgct gattaaggat tttgaggtgt ttgataagtg ccttaagtgg
     1561 ctgctgggga tattggccgg agccggcacc gcctgctttg tggggctggt ggatgatata
     1621 atggatttaa aacccaaaca gaaattctta ggtcagatta tcgctgcttg tgccctgctt
     1681 gcaactggca ttcggccgga tttgcaacat gcagcagcat ttctgggatg gaatatgccg
     1741 gatagcatag aatttctctt tggagttttt attgttgtct tttttgttct gggggccacc
     1801 aattcattaa atctgctgga tgggctggat gggctgtgcg ggggagtaac agcgattatt
     1861 accgtcgctt ttctgctttt gtcgattcac cgggcgacgt ggggattcag cgaggccagc
     1921 gatccggttc gcattattgt atgtttgggg ctggtgggcg gagtactcgg atttctgccg
     1981 tttaaccggc atccggccaa aattttcatg ggtgacgccg gcagcatgct gctgggattt
     2041 gcgactgcat ctctgatgat tttgtttgct gaggaaattt tccgctggtg gatggcgtcg
     2101 attgtgattt ttggactgcc gatactggat acggcgacgg ctctggtacg aagatggctg
     2161 aataaaaaac ccctgttcgt ttcggaccgg gggcatatct atgatcaaat gatggaccgg
     2221 ggaataccgc tgaaaaaaac cgttgctatc tgctatggat tggcagggat gtatgccttt
     2281 attggtcttg ctatcagtca gattcgtaca cgttatgcaa tttttgtgta tacggctgta
     2341 attgctgcat ccgccctaat catctggaaa aaaggctttt tgaagatgga aggatacaga
     2401 ggagcaataa gaaagagtga ggattcagaa ataaagaatc agcaaataca gtcagagacc
     2461 taactgaata atcgatacag cagttatttt tccacaataa agcagacatt ggtggtagcc
     2521 gtgccaccga tattcagggt agcaacccgg ctggcatctt ttacctgata gtcccctgct
     2581 gtacctgtaa cctgtttgta ggcatccagc agctggcggg caccggtagc accaaccgga
     2641 tggccgcagc cgatcagccc gccggaagga ttgatgggca gcttgcctga aagttctata
     2701 acgccatcct ctatcgcctg ccagctttgc cccggcggag tcaggccaaa atggtcgata
     2761 gccatgtatt cggacgtagt aaagcagtcg tgcgtttcaa tagcatccaa gtcccagcag
     2821 tcagcaatgc ctgcgcgggc ataggcatcg gtaatagccc tgcgtgtcca gggcaacaca
     2881 taggactgat tttcggattc agcaattttg gcagcaaatt cgataggtgc cgttgtatgc
     2941 ccccagccgg caatataggg gagcgcatcc ggtgatatac cgcaacggcg agcatactgt
     3001 tcagcaaaag atttagaagc aagatacaga cagaccgcac catcggtaat ctgggagcag
     3061 tcggtcacct taatccggcc ggacacagac ggattgtact ggcctctggt gcaggcatgt
     3121 tcataagaca taaaccagtc gcgtgtttga gcgttgggat tgcgtttggc attggcataa
     3181 ttgattgcag caattcgtgc taaatagtca tcatccaatc caaaacgggc ttcatagaca
     3241 tcgcccagtt tgccgaacag cttgggaaaa ggaaactcca cacccgctga ttcacgctca
     3301 taccaggcgg ctgtccccag atagtcgccg cctttggccg caggaacgga tttcatcagt
     3361 tccacaccca tcagagcaat gcagtcataa agcccggctt ggatgtgcgc catcgccgac
     3421 agtgccgcca aagcactgga tgcacatgcg ccttcaaacc gcatcgcagg caggcctctg
     3481 aaagccggat caaaatctac aaagaatgct cctaaatgcc cctgcatgca gtacagttct
     3541 gcagcaaagt ttccgataaa ggctgcttta atgtcctttg gctcgatttt ggtggtcatc
     3601 agcgatcctt cataggcctc acgcatcata gcagaaatat gcttgttttc acgagcccaa
     3661 ttgcgggcaa aatcggtctg ataaccgccc agaatgtata ctttttgttc actcatacca
     3721 ttcactcata aaaaaatgat gaatagagaa tgaagaaagt taatctttct tcagcttgga
     3781 ttcttttaca attgtcgtca ggatggaaat aatctcattg gattccttta ataactcttt
     3841 caagtgcagt tctgtaacaa taccggtcga ttgatataac cgcagccaat cataggtttc
     3901 ccatgcctcc ttaagacatt tgaaatccgc acggctcggg gcggcattgg cttcttcaag
     3961 attagccctc aacagatgtc cctgagcgaa taagctgacg gcacaagaac tcattggccc
     4021 gatctttttt atcaaaaatt gataaagtcg aatcattctg tattctaaat tctaaatcag
     4081 ctttcgctta tttggcgggg aagtatttgc cggcgttaaa aatataatgt tcgcaggtgt
     4141 catcggtccg cagcaattct ggaacaggca gcttttgcat tttaatcagt tccatcgccc
     4201 tgtcttttcc gccgagcata taaaccatta aactgggact tgcccagttg aagccggtac
     4261 tcatcacgcg gtcgatgccg gttatgccgt attctgcatc tgttacttct ccagcaagca
     4321 tatacccgta agcgatataa gtagccagaa tttcgcggac gatatcggct tccgtccctt
     4381 tggcagcaac gatggtgtca aaggcttgac ggtacttggc aagatgaatt tggtctttgg
     4441 ccgtctcaac aaaccgcaca tgggggtgaa acgacggaat atagctgccg gttgcagggt
     4501 caatatacat aaagtccccg ctttcgagtt ttttgtagaa accgcccttg tcccgtgttt
     4561 tattgcccag atgccctgtc tctatcatct tttcaatata tgcaggcagc acgagtttat
     4621 gatggaatgc atcatcggta tgagctgcaa gactgtggat aatggcttta tggatatcca
     4681 gaccgaccaa atccaatgtt gccaacggcg gcatcagccg acctgtatag ggaccgataa
     4741 gataatccat catttcgacg ctgaattctg cagcaagctc cgtaatccgg gcaaatagaa
     4801 caaatgccaa gcgattgccg gcaaagccgg ccgtgggctt gacgcggatg acggcacggc
     4861 gaagtttcga cgagcagaaa tctgaaataa aatccacaac agccggatcg gtttcagagg
     4921 tgccgcagat ttcacaggcg gtcattttgc cggggggatt gtaaaaatga gtggaaagga
     4981 aatgcctgcg gaaggatgcg gaccgtcctt tcggaagttc atccagcagc aggctggaag
     5041 taacagagct gacaattgca tccggccggc gcaagttgtc gaccctctca tacagtccct
     5101 gcttgatgct gtaatcttca gcgacacatt cgataatcca atcggcctga cggcaggcat
     5161 ccggcaaaag gccttcgtag tcgccgcagg tgatgtgttt ggcgattgtc tccgagcggg
     5221 cctgtttttg tgcacgggca agtcctttca gagaaccggc cgaagtacgg gaaaggaaaa
     5281 aaatcttgat gccgttttgg gccagaagcc cgccggttaa tgagccgatc gtcccgctgg
     5341 caccgagaat aagaacagtt tcaatcttca tttactttgc ctcgaggtat tagatgataa
     5401 gtcatcgctg ctttcatttg cggttttgac gactgttaat gtctgctgct gatatattat
     5461 tgcggcctgt caagcatttc ataatgctga tgggttggga tgaattcctc ccgtaaaaga
     5521 ccgatctggt acaggtcgat tcgctgacta ttgacaaccg catgccgacg cagggtccct
     5581 tctatctgaa agcccagttt ctcaacaaca cggaaaatag ccggattgtt ggtgcggata
     5641 tgtgcccaga ttttctggat atcgagcttc tcaaaagccc attggaacag cacagaatga
     5701 atttccgtac ccagcccctg tccccagaag gtcttttcgc cgatgacaca gtagcattgg
     5761 gcggtcttgt tccggcggtc aatatcgata agacctgtga tccctattgg caccccgtcc
     5821 aatgattcaa cagcaaaatc cgccctgtca ttccggccgg ccagcgattg aaaccattcg
     5881 attgttttgt ccagttcgaa ctgctcatca ataaccagcg ttttgcggac aagcgggtca
     5941 ttgtaccatt tgaccttgag cgtcatatcc tgttcagtca gcggcctgag ccgtacatgg
     6001 tcggcggtaa tgatattatc cactttcatt ttttccgcag gccttacaaa agtgcataca
     6061 gttcatcaga aagtttcacc ggtttttgac agaagcaggg tatgttctgc atagtcatac
     6121 tcaagacagc aaatcatagc aggttcttcc gataccagtc gatggccttg gtcaaaccct
     6181 cttggaacag gacgaccggc tggaagccga tcagctcccg ggccctgtcg atggctgcca
     6241 gagagtgctt gacatcgccg gggcggggat ctgcgtacac cggccgaacg ttggtgccgc
     6301 agatacggtt aatcaggtca ataatcgcat tgacggatat ccgctcgccg caggcaacat
     6361 tgatgacctg accgcttgtc tttttcgcac gagccgccag cagattggcg tgaacaatat
     6421 tatcgacata ggtaaaatca cgggtttgct ccccgtcgcc ataaataacc ggcggctggc
     6481 ctttcaggat atgggttaca aaagccggaa tcgctgcagc atactggctg gttgggtcct
     6541 gatacgggcc gaagacatta aaatagcgca gtgaaattgt ctccagacca tagcactggg
     6601 caaaaacaga acaataatac tcgccgaaaa gcttggctgc tgcatagggt gatttcggag
     6661 caaccggcat tgtctcgact ttgggcaaca cttcactgtc gccgtaagcc gaagagctgg
     6721 cggcatagac aaaccgcttg attttcatat cgcgggcagc cagcaggaga ttgaatgtgg
     6781 catcgacgca gtgatggtgc gttgtcgccg gatccgccac gcttttgggc acagacggca
     6841 ccgccccctg atgcagaact gcatctatat ccttcattgc ccttttagca atgtcgaggt
     6901 cgcccatatc ggcttcaata aaatcaattt tatcgatgat atctgccaaa ttcgattttt
     6961 tccctgtgat gaggttgtca atcacgcgga cgaaacagcc gtcggcaaca agcttgcggc
     7021 agatatttga gccgatgaag ccggctcccc ctgttacaag aaacttttgc ataaatgtac
     7081 actcctaagc aacagaacga actattctat ttcacacaag attcctaaaa aacataactg
     7141 ctgaagatat catcaagggt ctgtctgcgc cgaacaagct taaaacagcc gtcttcgtca
     7201 gcgagaacag ctggtgcagg cgtaataaaa ggcggagtca gcaccagcgt ataggccccg
     7261 acattgtcga tacagatata atcgcccggc tcaggcagag acgccgttat gtttcggagg
     7321 aggatatctt tttccatgca ggttgccccg accacactgt aggtatgtat gggcggatcg
     7381 gcatttcggg ttatgacctg atgcggcaga ttgagggcgt gaaaggtggg cttaatatga
     7441 aaggcgctcc cgtcaactgt aataaataaa tcgccgcgga tggatttgac cgaaacaact
     7501 ttggtgatga aacggacggc atcggctgcc attgccacgc ccggctcaat aaccaaagcc
     7561 ggagttttct tccgaaccca gctgtttctg cccagaactg aacagattgt ttcagcgtag
     7621 tcatcaaagc caggcacatc cgtaaacggc atatccggcc gcatccggcc gaaaaaaccg
     7681 ccgccgatat tgagatattc aacagaatcg ggaaaaaact cttcggcaag acggcacaaa
     7741 gtttcggtaa ttacctcaaa acaccagcgg cttcggtcgg cagttgaggt atggccgtgc
     7801 agactgacaa tctgcagatt gggcagctgt tttaaatcct gaatatttag ctttgattta
     7861 aattcagcgg gatccaatcc aaaacggccg gtcttgaggg cgttttggat atgcgactgt
     7921 cccgctgcat ccgacagggc aatattgatg cgaaggccga ctcgaaccgg acggcgggga
     7981 tcggctttgg catagtctgt aatcaactgc agttcggatg gactgtcggc attgacaata
     8041 ctgccctttt cgacagcttg cactatatct tcggctgtct taacaggacc gttgaaaata
     8101 attttctggg gatcgtggcc gattttaacc gccaagtcat attccagccg gctgaccact
     8161 tccgcccagc cacccttttc cttaattatg ccgcacaaat aaggaagata gttggttttg
     8221 taggaatagg ccagaataaa cggctgatag cggcttgaaa acgccgcggt caggtcctta
     8281 aagttggact cgaattgttg tcggctgacc acataaaacg gcgttccgca tgcaatcgac
     8341 tccattttca aaatatctga acggctcagc attattgcgg tttattcgga tttcgtattg
     8401 tcggccttgg tacccataaa ctcatcgaca gtagcatacc cttccttggc aacacctttg
     8461 cggcttatca catgccagag ggtcagccag aggattttta aatccagcca aaacgaacgg
     8521 ttgtcgacat accagacatc gtattgaaac ttctgttccc acgtcatatc attccggccg
     8581 ttgacctgcg cccagccggt aatgccgggc ttgacctcgt gtcggcggtt ctgctgggga
     8641 gtatagcggt ccagatactt ggccatcagc ggacgagggc ccaccagcga catatctcct
     8701 ttaaggacat tcaacagctc gggcagctca tccagactgg tcccccgcag ccaaagaccg
     8761 aaaggggtca atcgcttttc atcgggcagg atatttccct gctcgtcatg ggtacggctg
     8821 atcatcgtac ggaatttata gatagtaaag agttttccat gcaaacccgg acgttgctgg
     8881 caaaaaagaa tcggtgagcc gagtttaagt cgtaccagca aagccgtaat cagtatgaga
     8941 gggcttaaca gtaccagcaa gaccaatgac acaactatat caaagaaccg cttaaccata
     9001 catctctgca aaaaggaatt tcttggacaa ttctttcttg aagggtacat tccgttttcc
     9061 ggaagcggtc ttatcttttt gatgccacat ccttatttac ttactggggt ttcccagatt
     9121 ctaatgggtt tgctggctcg gatatcagag gtagcaaaga aacggctgtc gggcgaatag
     9181 gcaattttgg aggtcaaatt gctggtagga gtgcaccaat gaaaaaccgt gtcgctgccg
     9241 gtttgaagca gataggcctt gggattgatt gttgcaacgt aatagcggcc gtcattgctg
     9301 aatgccaagt tgagaaccga atagtcattg gtaaccgggc gggtttcccc gggagcgagc
     9361 agattaacct ttttgagaat gtttaggtcg gctgcatcga ttttatacaa aatgccgtcc
     9421 cgatcgccgg ctaaaagata ttgaccatcg gggctgacag cagcactgca gaactctttg
     9481 gtatctgcat aaacaatatc ctgaacaatt ttgccttgat gcaaatccac agccccaacc
     9541 cagccggctt ggcttttgga gccgacagca taaattacct gattagagct tgctgcaaaa
     9601 tcaacaattt tatcggtctt ttcgcttatg gagattgtta cggccggccc gattaattca
     9661 ttttcaatat ccagtttcgc gaattcataa aggattttgc cggctgaatc agcaggattt
     9721 ttgcgcatca gcaaaagaac ctttttgtcc ggtgaaattc tcaggaaaac agactgatct
     9781 tcccatccaa ctgacagctc cttctgccgg cggccggcag gcaggtcata gacagtcagg
     9841 atttttttac ctttttcagg aacagccgcc aagcgggatg catccagcac ggcagccaga
     9901 caggaaacag ccctgaagtc gcctttgggg ctgggcagat tgttccagtc ccagatgcag
     9961 gtgcgtccgt cggaaaatac acaaaccagt gaatcgccaa ccgtttccag aagcgctaca
    10021 ccggcattgt cgcgaatatc gagggaaaat tgcacggatt tcggacgaag cagatgccga
    10081 agaattgtca caacaataag aatggcaaat actccagcaa ataatttaac aggactcttt
    10141 tgctttttca taaagaaacc ttatatggca gactgccggg actatttacg gcactgaaac
    10201 acaggattta tgcaggcgta aactgtgaaa taaagtccgc cgttctgcgt acagtttttt
    10261 caccggctcg aggatgaaga ggcaggttga ctgtttcagc ggcggctttt tcagcttccg
    10321 gacacattcc caatgtataa tcataagccg ccagaggcgt ttcctgagga tggagcggcg
    10381 attcaaacca gcttccaagc tcaatgcccg ccgcggcagc tgtttccaaa gcctgctgct
    10441 tttgtttaat ccgcaccgga tagcgcacca gaaccggatc cattacgctt ttatcatagc
    10501 agcggatcgg ccagcccttt tctgacagca gatggtcata aaggtcagcc atttttcttc
    10561 ggtgacaaat attgtcttca agcttattga gctgtttgat gcctgaacgg gcttgaacag
    10621 cactcatccc tttgaagaag ccgctgggca tagaaggcac gaattccgca gatgaagagg
    10681 acccgacaac aagccctttg cgtgttaaaa accgaaaaag cgtttgagcc agtgccgtcg
    10741 ttcgggggta aatgaaagca cggtaaacag ccagctgtgc tgccagcatc agcacatctt
    10801 tggccgacgg ttcagttgcc tcggcagcat ataatcgctc aatcttttgt gccaaaccgc
    10861 tgtcatttgt aatagccatc cctccaaggc cggttgtaaa gggcttgttc cactggaagg
    10921 agaagtaagc cgccctgccg aacgtaccga ccaattgtcc tttatagcgt gaccccagcg
    10981 ccagacagca gtcttctata acggggattg cgtgtttttg ggcaatctgc agaatacgct
    11041 ccatatcaca cggataaccg tatgtgtgct gagcaattat aagtttggtt cgcaaggtaa
    11101 tcttctcttc aacgaagtcg ggaaccatat tgaatgtatt cggctctata tctgcataga
    11161 ccggctttgc tccgacatat ttaatcgggt tgacatccat cacacacgta tagccgggca
    11221 aaatcacctc atcaccgtct ccgatattca atcccctcaa aatcgcatag agggccaccc
    11281 gggctttcca aaaggaaaaa gcatagggac aattcagcca agcagaaaag gattgttcaa
    11341 atttatccat ttttgttttc cacaaaagga gttttgcacc attggaggtc agttatgatt
    11401 tgagaaaatc aaaatatagc gattcatttg ccggccttca ggggtagtat atgatgattc
    11461 aagagtaaaa cccatttttt gataaaaacg attgaccttg tcattgccct gggcatctgt
    11521 ggttaaaaat atacctttgc cgcctcgttt tttaacttct tgaacaaacg cattgaccag
    11581 agccgcacca atacctttgg cttgaacatc gggcgaaaca gcgatactgc ttaataaagc
    11641 caaagcgtca gcgtgggcag gagaatcacc gcgataaaag acggcacgaa atagccgctt
    11701 tatgacggac ggctttttaa tcactgcgga aatgcttgcc agacaaaaag cccaccattt
    11761 gcgcttgagc aaccgcttaa aataacctgc tgggaccaaa gggcctaccg taaccgctat
    11821 cacttggttg caatcgctat ctacggcaac aaaaccaatc ccaataggat cttctgcaaa
    11881 tgattgataa aactccttta gaaatcgcgg gcctaaaaaa cttaaaaaaa agccaggaaa
    11941 tgctttgata tggatatcaa ctacaaggtc aatatgcgat ttttgaagag gaataatttt
    12001 ataattcatc tctttatgca caacagaatt agttatcgtt gaaaaatact cgaagcagaa
    12061 tcaactgatt accagccgag cttgaattct ttgacaacct tatcaatttt atcaattctt
    12121 caacattata ttttgaaaaa caacgcaaga ttaaccatca gcttttaata gttagccgcc
    12181 aacgattgct gtgaagcatt tggccgccgg ctgacctgag aaagaagaat atgctcaatc
    12241 ttggcggctg caatttcaaa tgataaatac tgctgataaa attgttttcc gcgatgtccc
    12301 atcgcttcta actctgattt tgacattaca ctgaattgac acactgcgtc ggcgatactc
    12361 tgcggatttt ccggttcaca ggccaaaccc gcctgcgctt cctgaacaag agctgacgca
    12421 tcccccggaa ctgctacaag tatcggtcga cccgctgcca aatacgattg tattttcgag
    12481 gggatggtaa ttttaaacag cggatctttt ttcagatgaa caaacaaaac atccgaaaga
    12541 tttaaaatat caccgatttc cgacaccggc cgacggggca gaaacaggac attggtcaag
    12601 cccatgtctg cggccttttg tttcagttcc tccacacaga caccgctgcc gatgaagaca
    12661 aattgaacct gcggctgccg atctgcgact atttttgccg catccaaaac cggctccagc
    12721 gcctgtgcct tgcccatatt gcctgcaaaa acgacattaa atcgccccgc cataccaagc
    12781 gactttgcca attcctgatt tttttctgcc ggacaaatct gggcttgatc acaccaatta
    12841 tagaccactt caatcttatt ttccggaaca ccgcgttcaa tcagtcgctg cttcatgccg
    12901 ggggctatcg taata
//
LOCUS       DAOTBO010000079        12824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4113_length_12824_cov_5.584854, whole genome shotgun sequence.
ACCESSION   DAOTBO010000079 DAOTBO010000000
VERSION     DAOTBO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_12824_cov_5.584854"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..519)
                     /locus_tag="PLE88_09295"
     CDS             complement(34..519)
                     /locus_tag="PLE88_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76697.1"
                     /translation="MKSTRLLILLMLMTALAAPIFANINGAIFTTESQCDGTNVNLFN
                     SKEAVYLDGGPRKPGAAGLPDGYYYIQVTSPNGQLLGISPTAAVHVTNGEFDTCYQLW
                     SILIKASDGSMGYDTTPNNGGVYKVWATPEAYFSPGDGTNGFINAWCKTDNFKVTVPL
                     G"
     gene            complement(1013..1387)
                     /locus_tag="PLE88_09300"
     CDS             complement(1013..1387)
                     /locus_tag="PLE88_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76698.1"
                     /translation="MLVFLIVKPKQKRQNNNVKFKLYTGKDLVMSAKASNNYQQQIIQ
                     NYYKNRDTILLTRLQEIVTDLYLAETGVVRKKLWKRVEATLDKMEIPQSVKAHIIQQK
                     DVQILAKNLEEWLRTGKDKKNL"
     gene            complement(1500..1865)
                     /locus_tag="PLE88_09305"
     CDS             complement(1500..1865)
                     /locus_tag="PLE88_09305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM76699.1"
                     /translation="MNSLMERRREERLRYSWPVWFAEDFDDILTQGQMVDISSRGAAF
                     TCYADRCPTNGEMITARFSVPKFEDSDSFDMENFIRDGRICRIDELGPYIRRVAIQFS
                     EPLPFNPAACHRQERLVCV"
     gene            2285..3700
                     /gene="ffh"
                     /locus_tag="PLE88_09310"
     CDS             2285..3700
                     /gene="ffh"
                     /locus_tag="PLE88_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOM76700.1"
                     /translation="MFDALTNKLNGVFRSLSGRGRITEANVSDAMRQVRQALLEADVN
                     YQVAKQFCKDVQQAAMGAEVIKSLHPGQLMIKIVNDELTRLMGPVDPKVYYVSPGPTI
                     IMMAGLQGSGKTTTAAKLAKMIAEKDKKRPLLVADDLQRPAAIDQLCTLGMQIGVDVY
                     KEDGVRDAVQVAKNGLKHAKAGGYDAVILDTAGRLHVDEEMMNEIAAVAKAVSPHQIY
                     LVCDAMTGQDAVNSAKEFNERLELDGVILTKLDGDARGGAALSVKAVTGKPIKFVGMG
                     EKLDNLEPFHPDRMASRILGMGDVVTLVEKAQQQFDAEQAQKMQVKMAKGTFGFDDFL
                     KQMQMLRKMGGLSSMLKLLPGMGGIGNLELDDREINKIEGIVHSMTLQERSNPDIIDS
                     SRRRRIARGCGRDVQEVAGLIKTFMRSRDMMKMLAGGGGGMTALKSLFSGKTDVISAM
                     NSMKKVKQRSKRKRPPRRKRR"
     gene            3704..4021
                     /locus_tag="PLE88_09315"
     CDS             3704..4021
                     /locus_tag="PLE88_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76701.1"
                     /translation="MTIEEYLKSLEPFIDNPYGRQVRNQFCSITGSSELAVLQSPRRD
                     EYEQLCRAVAIMTQAEKINAALLTDEQIERIAEDAKADKALIAIFINGFAIAEAKTKK
                     IKR"
     gene            4167..4559
                     /gene="rpsP"
                     /locus_tag="PLE88_09320"
     CDS             4167..4559
                     /gene="rpsP"
                     /locus_tag="PLE88_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOM76702.1"
                     /translation="MAVKIRMTRMGRRHRPFFRINAVESRTPRDGRILEKLGHYDPLE
                     KNADKQYVLNRERIEYWLGQGAIPSDTCAEIFKRQLSITCPHYEAKLQRRARARAVAR
                     KLGKPFTKAERLAAGGAGAGEGQAAKEE"
     gene            4569..5279
                     /gene="trmD"
                     /locus_tag="PLE88_09325"
     CDS             4569..5279
                     /gene="trmD"
                     /locus_tag="PLE88_09325"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOM76703.1"
                     /translation="MRIDVLTLFPEMFASPLSYSILKRAQEQNLVEIVLSNIRDFALD
                     SYRKVDDKPYGGGPGMVMMPGPVFDCVEHVQQQASEPGHIILLTPQGTPLTQEKVIEL
                     SRRPRLVLIAGRYEGFDERIRMGLAAESISIGDYVLSGGELAAMVVIDAVVRLLPGAL
                     GDEDSAAEESHRGGLLEYPQYTRPEEFRGMRVPSILLGGDHGKIAQWRHQQRLERTRL
                     WRPDLYEKYLKSNPDTKE"
     gene            5319..5675
                     /gene="rplS"
                     /locus_tag="PLE88_09330"
     CDS             5319..5675
                     /gene="rplS"
                     /locus_tag="PLE88_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOM76704.1"
                     /translation="MLDSVESKSIRKEIPYFEVGDTVDVYCKIQEGDKTRTQIFGGIV
                     IARKGRGMNETFTVRRMVGDEGVERIFPLHSPNVLDIRPIRSGKVRRSKLYFLRKRVG
                     KGVRLSQRHSDHTVTK"
     gene            5693..6085
                     /locus_tag="PLE88_09335"
     CDS             5693..6085
                     /locus_tag="PLE88_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOM76705.1"
                     /translation="MLLPWVRKKLLNAPAQLGHWGQNQAERFLKRRGLRTIARNWAVQ
                     GGELDLVMADADGMLIFVEVKTRQSEDFIPALAAVNREKKQKIIRTAKRFLRHFKISG
                     RPLRFDVVTVVIGPSGPPDIRHYPNAFC"
     gene            6087..7364
                     /gene="truD"
                     /locus_tag="PLE88_09340"
     CDS             6087..7364
                     /gene="truD"
                     /locus_tag="PLE88_09340"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOM76706.1"
                     /translation="MTTDSSSRSNGEDSVSPLQPQLPLLTAGMQGIGGNIKTIPSDFV
                     VEEIPLYEFSGTGTHVFFYVQKKGLTTAETISRLSQALGVRRFDIGYAGRKDSNAITR
                     QWFSLEHIDPEKLKQLKLNKIKILDITRHNNKLKVGHLKGNRFTIRIRNLHCPLQEAE
                     ALAQKILDVLCRRGVPNYFGPQRFGYRYDTHLLGEALVKNDIQRFFDFLLGHPELESQ
                     PEFVPARTLYHQGNFQAAYEAWHPGFRDHRDGLKAIIQSNGNLNKALRAMDRRLLSLF
                     VSAWQSDLFNEVLAKRIDKIDIVLEGDMAYKHDNGACFRVENPSAEQPRCAAFEISPT
                     GPLLGARMMELTGPAGEIENPILQKTTLAPEDYIRIKRYGGAGGRRPLRFLPKNIRLT
                     TSRDDHGDFLQLSFELPSGCYATVLLREITKKQ"
     gene            7527..8108
                     /locus_tag="PLE88_09345"
     CDS             7527..8108
                     /locus_tag="PLE88_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOM76707.1"
                     /translation="MKTRTVVIVAVVVAAVLAPMVMAAPDAAGPRGQRGQGGPEGMQG
                     MRGGFGFGGMMGGDTLSMLLGRAAEDIGLTDSQRTKLEAIREENREKTQELQTAVRDA
                     TMALNEAVEEGNDAKITAAGKALGDAYTKQALHRAAVTKQVKAVLTQEQLTKLQDLRT
                     QMRERMMQRRQEGGGPQAGQDQPRGQRRQQGQQ"
     gene            complement(8197..9042)
                     /locus_tag="PLE88_09350"
     CDS             complement(8197..9042)
                     /locus_tag="PLE88_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76708.1"
                     /translation="MTKHIRKSGFTLIELLVVIAIIALLLSIIIPAIGRAKVYAQKVM
                     CSSNLRQQCLGTTLYSNENDSYVPTCGLGPWLWDISFFATNQLSYYAGFSDNKIFFCP
                     ANRIKKSTDARFWQFSWLGSTGGPYPNEVPLRDESVLTVAQQKYYYRVPPTLYMFDKY
                     NSSTGASILDRYLVTGEEANWIRKLSNVKASGSKTMLMDVVISQNNNWKFFGIDGGDI
                     VGLSGGTLTDDSNHKSRQMIVVSPTAQGPKPEGGNVGYADGHVDWRRFEDMKHRYTRG
                     MWFWW"
     gene            complement(9278..11047)
                     /locus_tag="PLE88_09355"
     CDS             complement(9278..11047)
                     /locus_tag="PLE88_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOM76709.1"
                     /translation="MQGYNESIMLSNARRLLLEARTADGIWQGRLSSSPLATAVAVFA
                     LHCADAAAHSELIDRGLGWLAAHQHPDGGWGDAEILDPPNLSSTLLCYAALNAINKEK
                     YRTAVDSAARWLVQKAGGLEPDSLAKAVYRSYGTDRTFAVPILAMCALADVLGKNGWR
                     YVKALPYELAVLPRCLFRWLNLTVVSYALPALIAIGQVKQHFSPSANIIVRGIRRFVT
                     APTQALLERLQPTHGGFLEAVPLTGFVLMSLAAMGLTGSKTAQKAAVFLCRSVRNDGS
                     WPIDTNLSTWVTSLAVRALLEGQQTLLSESERLNIAQWYLDQQFRSRHIYTGASAGGW
                     GWTNLPGAVPDADDTAGALITLHRLGIRSQPLLESVRNGVKWLLDMQNSDGGIPTFCR
                     GWGKLEFDRSCPDITAHAVTAWQLWKDAFDSQFNHRIHSSIQKALCYLYSAQNKDGSW
                     TPLWFGCPFSDRFENLIYGTSMVLQTLVLLEDYPLSSIMGKKGTEFLLDAQNKDGGWG
                     AQKGYPSTVEETSLAIRSLIKMELTEAEENQIEQGLKWLTEQTKEGVCFKPKPIGLYF
                     ARLWYAEHLYPVIFLSGINGYAL"
     gene            complement(11031..11924)
                     /locus_tag="PLE88_09360"
     CDS             complement(11031..11924)
                     /locus_tag="PLE88_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOM76710.1"
                     /translation="MNICQTLLESASRSRFLLKERKSEIAEVILCRQNKDGGFIGKGA
                     QSDLYYTGFALLSLLALEVPFNRAQVVSFLNTADTGTDCDLAHLAALIRCRRLMMPSN
                     RQTQNAFRRRLESFRCSDGAYHHLHAANQGSAYGCFLAVGAYQDLDAALPDGSRDKML
                     ECLERLNKGQGGYFNEPDIPAPSVPATAAAIVVQRALTGSVKSSKSVQWLMSCYDEGG
                     FRVMPIAPVSDLLSTAVAVFALSAAGADLKSIRQPCLCFTDMLWKGGVGFCANPFDSI
                     SDTEYMFYGLLTAGILCAGIQ"
     gene            complement(11899..>12824)
                     /locus_tag="PLE88_09365"
     CDS             complement(11899..>12824)
                     /locus_tag="PLE88_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOM76711.1"
                     /translation="AGPVNLSQLGQKVRDWFDADTLRAVFNEADGPAAALALAWMAED
                     GKRYRPMLTAGIYAVLTGTAVEQIPPAVKQAAIAVECFHKASLIHDDIEDNDAMRYNQ
                     PTLHQRYGIPSALNAGDYLLGKGYQLLTDLNCQDAQRMRILQIAAQGHRTLCIGQGKE
                     LDCVWAKTAPGRQKLLEIFLQKTSPAFAVALKIGAVLGGADEPLLKVLDAYSDAVGIA
                     YQIRDDLYDHLAGQAGQPLSILSALQLEGQAKADIEARKMLAEFRKTAVECLEPIVQS
                     DCKAFLCKVAAKLLDGTELMECCDEYLSDAS"
BASE COUNT         3459 a         3098 c         3029 g         3238 t
ORIGIN      
        1 tcaatgtcgg aaatcctaac acacgaaatg gccctaaccc agggggacag tcaccttgaa
       61 gttgtctgtt ttgcaccaag cattaataaa accatttgtt ccatctcccg ggctgaaata
      121 cgcctccggc gtagcccaga ccttataaac gccgccattg ttcggagttg tatcataccc
      181 catagagcca tcgctggctt ttataagaat actccaaagt tgatagcatg tatcaaactc
      241 accattcgta acatggacag cagctgttgg gctgatgccc agcagttggc cattcggcga
      301 ggtaacctga atataataat atccatccgg caaccctgca gcgccgggct ttcggggacc
      361 gccatccaga tatacagctt ctttgctgtt aaagaggttt acattggtgc catcgcactg
      421 actttcggta gtaaaaatcg ccccattgat attggcaaat ataggtgctg ccagagctgt
      481 catcaacatt aataaaatta atagtcttgt acttttcata attttctccc tttccgcttt
      541 taattatatc tctcaattta catgtggccg cctcttgtat tttttagaaa taaaattttt
      601 tcgtttccat aaacaccaag aagattcgac actttttccc cattcaaaaa ctgtctgtta
      661 tcgataaaca cctcctttcg ggctgcgggg aatatccaca aattacatac ctattttaat
      721 ggcaagcgaa cgattttata tataaaacct tttttgagaa attttcagtt ttccggaagt
      781 tcgagaggga ttgttgatgg ctaatgtagc gaaaagataa aaattggtcc ctcatctccc
      841 atcctttttc aacgaataga ctacagataa atcattaaca ttataacagc ttcgtatggc
      901 cagaacaaga aaaaacgtat tacgataggg ggaaatattc aaaactacgg aaaaggacaa
      961 tatctgtggt cagtatatca ttgcaagatg tattccagac aatttggatg ccctataaat
     1021 tctttttgtc tttccctgtc cgcagccatt cctcaagatt ttttgccaaa atttggacat
     1081 ctttttgctg gataatatgg gctttaactg attgaggtat ttccatttta tccaatgttg
     1141 cttcgacgcg tttccagagt ttcttgcgga caacgcctgt ttcagccaaa tacagatctg
     1201 ttacgatttc ctgcaaacgg gttagaagaa tagtatcgcg gtttttatag taattttgaa
     1261 taatttgctg ctgataattg ttggatgcct tggctgacat tactaaatct ttccctgtgt
     1321 ataatttgaa ctttacatta ttattttggc gtttttgttt tggctttaca atcagaaaaa
     1381 caagcattct tttgattttt caaaaaaata aaaaagcccg tacgctcctt tgctgtacgg
     1441 gcttttcttg taactaaagg gggctccaaa ctgtcccaag gcagtttgga tataaattat
     1501 taaacgcata ccaaacgttc ctgtctatga caggcagccg gattaaaggg aagcggctcg
     1561 gaaaactgaa tagcaacccg gcgaatgtac ggtccgagct catctattcg gcaaatcctt
     1621 ccgtcacgga tgaagttctc catatcgaag gagtctgaat cctcaaattt cggcacactg
     1681 aatctagctg taatcatctc gccatttgta gggcagcgat cagcatagca agtaaaagca
     1741 gcccccctgc tggatatatc aaccatctgc ccttgtgtta aaatatcatc aaaatcttct
     1801 gcaaaccaga ccggccaact gtaccgcaat cgttcttctc tgcgacgttc catcaatgaa
     1861 ttcatccgag taatctcctg tcataaatac tgtttctttc cagcggatcg cccattaaaa
     1921 acactttttt actcggttcg gccagccggt tacttaactt gtgaataata aacaaacttc
     1981 accattttgc ctgttgcttg tctaattagt gcaaaaagtc tgaaaacatt ttctgtttaa
     2041 tcaccatttt tttaatatgt tccgataacc tcatatctaa acttacaacc tctattatat
     2101 ctgctataat agcatcccat gtaatcttac tcccatcgtc attaagacct gcttaaaata
     2161 cgacccataa tcaggatttt tggcttgaaa aaactatctc aatcatctaa aatttttcgt
     2221 tttcattaaa tatgttctgc atggcaggca aacttgaaat aagccgggaa ctttaatttc
     2281 gaatatgttt gatgcgctaa ccaataaatt aaacggagtt tttcgctcgc tttcaggccg
     2341 tggccgcatt acagaggcca atgtctcgga tgcaatgcgt caagtgcgcc aagccctcct
     2401 tgaagcagac gtaaattatc aggttgccaa gcagttttgc aaggatgtgc agcaggcagc
     2461 gatgggggca gaagtaatca aaagtttgca tcccggccaa ttgatgatta agatagtcaa
     2521 tgatgagtta acccggctga tgggacctgt tgatcctaag gtttattatg tttctcccgg
     2581 gccgacaatt attatgatgg caggtcttca gggttccggt aaaaccacaa cagctgccaa
     2641 attggccaaa atgattgctg aaaaggataa aaaaaggcct ctattggttg ctgacgacct
     2701 ccagcgtcct gcggcgattg accagctctg tacgctgggc atgcaaatcg gcgttgatgt
     2761 ctataaggaa gatggtgttc gagatgctgt tcaggtagct aaaaacggac tcaagcatgc
     2821 aaaagccggc ggctatgatg cagttattct tgatacggca ggccggttgc atgttgatga
     2881 agaaatgatg aacgagatag ccgctgtcgc aaaagcagtc tctccccatc aaatttatct
     2941 tgtctgtgat gcaatgaccg gacaggatgc cgtcaattct gccaaagaat tcaacgaacg
     3001 attggaactg gatggcgtca tccttacaaa gctggatggc gatgcacgcg gtggtgcagc
     3061 attaagtgta aaggccgtta caggcaagcc cattaaattt gttggaatgg gcgaaaaact
     3121 ggataatctt gagcctttcc atccagacag aatggccagc cgaatcttag ggatgggcga
     3181 tgttgtaacg cttgtggaaa aagcccagca gcaatttgat gcggaacaag ctcaaaagat
     3241 gcaggtcaaa atggccaaag gaacatttgg ttttgacgat ttccttaaac aaatgcagat
     3301 gctgcgaaaa atgggcggat taagcagtat gctgaagctt ctgccgggga tggggggaat
     3361 agggaattta gagttagacg accgggaaat caataaaatc gaaggcattg tgcactcaat
     3421 gacattgcag gaacgctcga atcccgatat tattgattcc tcgcggcggc ggcgcattgc
     3481 ccgcggatgc ggacgggatg tgcaggaggt tgctggttta atcaagactt ttatgcgcag
     3541 ccgtgatatg atgaaaatgc tcgcaggcgg cggaggagga atgacggcac tgaagagtct
     3601 gttttccggc aagaccgatg tcatcagtgc aatgaacagt atgaagaaag tcaagcagcg
     3661 gagcaagcga aagcgtccgc cgcggcgaaa acgcaggtga ttgttgacaa tcgaagagta
     3721 tttaaaatcg ctggaacctt ttatcgataa cccttacgga cggcaggtta gaaatcagtt
     3781 ttgttcaatc accggcagca gcgaattggc tgtgcttcag tctcccagaa gggatgaata
     3841 cgagcagctg tgccgggcgg tagcaattat gacgcaggca gaaaagataa atgccgcctt
     3901 gctgacagat gagcagattg agcggattgc agaagatgcc aaagcagaca aggcgttgat
     3961 cgctatcttt attaacggct ttgctatagc agaggcgaaa acaaaaaaaa ttaaaaggta
     4021 aaacgcaaca gggatttaat tttcagccgc agcaggaagt tgttgggccg ccggcggcct
     4081 atgaaaagca agacatgaaa acgtgttttg tgtccgcatt gggctgacac aaaaatttat
     4141 tatacacaca atgaaaggaa agtactatgg cagttaaaat cagaatgacc cgaatgggtc
     4201 gccggcatcg gccattcttc cgcatcaatg cggttgaaag ccgcactccg cgggatggac
     4261 gcattcttga gaagctgggc cactatgacc cgctcgaaaa gaatgccgac aagcaatatg
     4321 tgcttaaccg agagcgcatt gagtactggc ttggacaggg tgctattcca agcgatacct
     4381 gtgccgaaat cttcaaacgc cagttaagca tcacctgtcc gcattacgaa gccaagcttc
     4441 agcggcgtgc ccgtgcccgc gctgttgcac gcaaactggg taaaccgttt accaaagccg
     4501 aacgtctggc tgctggaggt gcaggagccg gcgaaggaca ggcggcaaaa gaagagtaaa
     4561 tccgctttat gcgaattgat gttctgacac tgtttcccga aatgtttgct tcaccgttga
     4621 gctattctat tctcaagcgg gcacaggaac agaaccttgt agagattgtt ctttcaaata
     4681 ttagagattt tgccctcgac agctaccgga aggttgatga caaaccctac ggcggaggtc
     4741 ccgggatggt tatgatgccg gggcctgtgt ttgactgtgt ggagcatgtt caacagcagg
     4801 catctgaacc gggacatatc attctgctga ctcctcaggg aacgcccctg acccaggaaa
     4861 aagtcatcga gctaagccgg cggccccggc ttgttctgat tgccggacgg tacgagggct
     4921 tcgacgagcg tattcggatg ggtctggctg ccgagtcgat ttccatcggc gactatgtac
     4981 tcagcggcgg tgaactggct gcaatggtcg taatagatgc tgtggtgcgg ctgctgcccg
     5041 gtgcgctggg tgatgaggat tctgccgctg aggagtccca cagaggcggg cttctggaat
     5101 atccgcaata tacacggccg gaagagtttc gcggaatgcg tgtgccgtca attctgctgg
     5161 gcggcgacca cggcaaaatt gcccagtggc ggcaccagca gcgtttggaa cgcactcgct
     5221 tatggcggcc cgatttatat gaaaagtatt tgaaaagcaa tcccgataca aaagaataac
     5281 aactaatatg aacaggagaa ttttccgtga acacaaaaat actcgattcg gttgaaagca
     5341 aaagcatccg caaagagata ccctatttcg aggtcggcga cacggtcgat gtgtattgca
     5401 aaattcagga aggcgacaaa acacgcaccc agatattcgg cggcatcgta atcgcccgca
     5461 aaggccgagg gatgaacgaa acattcaccg tccgccggat ggtcggggac gaaggcgtgg
     5521 aacgcatttt cccgcttcat tcacccaatg ttctggatat tcggccgatc cgcagcggta
     5581 aggtccgccg ttcgaaactt tatttcctgc gcaagcgtgt cggcaaaggc gtacgcctgt
     5641 cgcagcggca cagcgatcat accgtaacaa aataacaata cggatactgc cgatgcttct
     5701 gccgtgggtg cgaaagaagc tgctcaatgc gcctgcgcaa ttaggccact ggggacaaaa
     5761 ccaagctgaa cgctttctca aacgcagggg attgagaacg atagcccgca actgggcagt
     5821 tcaaggcgga gagctggatt tggtgatggc tgacgccgat ggaatgctca tttttgtaga
     5881 agtaaaaaca cggcaaagtg aggattttat cccggctctg gcagccgtta atcgtgaaaa
     5941 aaaacaaaaa atcattcgaa ccgcaaagcg atttttgaga catttcaaga tttcaggaag
     6001 acctttgcgg ttcgatgttg ttactgtcgt tataggtccc agcggaccgc cggatattcg
     6061 gcattatccc aatgcattct gctgatatga ctacggattc atcatctcgg tcaaatggtg
     6121 aagacagcgt ctcgccgctc cagccccagc tgcccttgct gactgccggt atgcagggaa
     6181 ttggaggcaa tattaaaaca attccatccg attttgtcgt ggaggaaatt cctctgtatg
     6241 agttttccgg aactggcacg cacgtcttct tttatgtgca gaaaaaaggg cttacaaccg
     6301 ctgaaacaat ttcacggctg tcgcaggcat taggcgtacg acggtttgat atcggctatg
     6361 caggccgaaa agattctaac gctattaccc gtcaatggtt ctccttagag catattgacc
     6421 cggagaaatt gaagcaatta aaactcaata aaatcaagat tctggatatt acacgtcata
     6481 ataacaaatt aaaagtcggg cacttaaaag gaaaccgatt tactatccgc attcgcaatc
     6541 ttcattgccc tttgcaggaa gcagaggcac tggctcaaaa gattttagat gtcctgtgcc
     6601 ggcggggagt tccaaattat ttcggcccgc agcgattcgg ataccgatat gatacgcacc
     6661 tgctgggaga ggccttggtt aaaaatgata tacagcgatt ttttgatttt cttttggggc
     6721 atcctgaatt agagtctcag ccggagtttg tgccggctcg aacgctgtat catcaaggaa
     6781 attttcaggc ggcctatgag gcatggcatc cgggttttcg cgatcatcgc gacggattga
     6841 aggcaatcat tcaaagcaat ggcaatctca ataaagccct tcgcgcaatg gacaggcggc
     6901 tgctgagtct gtttgtttcg gcttggcaat cggatttatt caatgaggta ctagcaaagc
     6961 ggatagacaa aatcgacatc gttctggaag gcgacatggc ttacaaacat gacaacgggg
     7021 cctgttttcg tgtcgagaat ccttctgccg aacagccgcg atgcgctgca tttgaaatca
     7081 gcccaaccgg tcccctgctt ggggccagaa tgatggagct aactggtcct gccggcgaaa
     7141 ttgagaatcc tattctgcag aaaaccaccc ttgctccgga ggattatatt cgtatcaagc
     7201 ggtatggagg tgcaggcggc cggaggccgt tgcgtttcct tccgaaaaat attcgcctga
     7261 ccaccagccg tgatgaccac ggggactttc tgcaattgag ttttgaactc cccagcggat
     7321 gctatgccac cgtattgctt cgggaaatta ccaagaaaca gtaaaaatac atctttattt
     7381 ttatctcaaa ttgtcctgtt tgtataataa aatattttcc tttcctgtaa cctttcaaca
     7441 aatacattca ttaacctgat gaaaagtccc cagattcggg ggaaaacaat aaagggttat
     7501 atttttataa tggaaaggaa acacaaatga agacaagaac tgttgtgatt gttgcggttg
     7561 tagtggccgc agtgctggct ccaatggtca tggctgcacc ggatgctgct ggtccccgcg
     7621 gacagagagg acaaggcggc ccagagggaa tgcaggggat gcgcggcggt tttggttttg
     7681 gcggcatgat gggcggcgat acgctgtcaa tgctgctggg gcgtgcggcc gaagatattg
     7741 gtttgaccga ttcccagaga accaagcttg aagctatacg ggaagaaaac agagaaaaaa
     7801 ctcaggagct tcagacagct gtccgtgatg ctacgatggc gttgaatgaa gcggttgaag
     7861 aaggcaatga tgccaagatt acggccgccg gcaaagctct tggcgatgcc tataccaaac
     7921 aagctctgca tcgtgctgct gtaactaaac aggtcaaggc cgttttaacg caggaacagc
     7981 ttacgaaact tcaggacctt cgcacccaga tgcgtgagcg aatgatgcag cgccggcagg
     8041 aaggcggcgg cccgcaggcc ggtcaggatc agccgcgggg tcagcgtcgt cagcagggcc
     8101 agcaataaag ctggaagcta tgaatatgaa aaaaaatggc tcgcttgtat taaaagcgag
     8161 ccatttttat ttccattcgt ttatcttctt ggcggcttac caccaaaacc acattccgcg
     8221 ggtatagcgg tgtttcatat cttcaaaacg ccgccagtcc acatgtccat cggcataccc
     8281 cacattgccg ccttccggct taggtccttg ggctgttggg gataccacaa tcatctggcg
     8341 ggacttgtgg tttgaatcat ccgtcagggt gccaccgctc agccctacga tgtcgcctcc
     8401 atcaatccca aaaaatttcc agttattgtt ctggctgata acaacatcca tcagcattgt
     8461 tttggaaccg gatgccttga cgtttgacaa tttgcgaatc caattggctt cttcacctgt
     8521 gacaaggtat ctgtctaaaa tcgatgctcc tgttgaactg ttgtacttgt caaacatata
     8581 cagggtcggc ggcactcggt aataatattt ctgctgtgct acagtaagga cgctttcatc
     8641 acgcagcggc acttcattcg ggtaaggtcc gcctgtcgag ccgagccagc tgaactgcca
     8701 gaagcgggca tcggtggact tttttattct gttagccgga cagaagaaaa tcttattatc
     8761 gctgaatccg gcatagtatg ataattgatt cgttgcaaag aagctgatgt cccacagcca
     8821 aggcccaagc ccgcaggtgg gtacataaga atcattctca tttgaatata aagtggttcc
     8881 caagcactgc tggcgcagat tgctggagca cattaccttc tgggcataca ccttcgccct
     8941 acctatcgcc ggaatgataa tgctcaacaa aagggcaata atcgcaatta ctaccaacag
     9001 ttcaatcagt gtaaaacctg attttcggat atgctttgtc atattctttg tctcctgcaa
     9061 attccttttg catttttagg cctctacaag aagcctcctt tatagtaaat ctggttaaaa
     9121 attaagatta gacaaatttt tttgtttttt tataaaaaaa tcctgattaa aaacatttgt
     9181 caattcaaaa aaattacagc atccaagttt ttattataat gtattgtaca atgttaaaat
     9241 agaaaaattt caaaatgctt ttaaacagct ttatatgcta taatgcatat ccatttatac
     9301 cggataaaaa tataaccgga tataaatgct cggcatacca taagcgggca aaataaaggc
     9361 caataggctt cggcttaaaa cacacccctt ccttcgtttg ttcggtcagc catttaagtc
     9421 cttgttcaat ttggttttct tctgcctctg ttaattccat tttaattaaa gaccgaattg
     9481 caagcgaggt ctcctccaca gttgaaggat accccttttg agcaccccag ccgccgtctt
     9541 tattttgagc atctaaaaga aattctgtgc ccttttttcc cattatgctg cttaagggat
     9601 aatcctccag aagaaccaat gtctgaagca ccattgatgt gccataaatc agattttcaa
     9661 atctgtcact aaacgggcag ccaaaccaaa gcggtgtcca tgaaccgtct ttattttgtg
     9721 ccgaatatag ataacataat gctttttgga ttgaagaatg gatacgatga ttgaattgtg
     9781 aatcaaatgc atccttccat aattgccatg ctgtaacagc gtgagcggtt atatctggac
     9841 agctccggtc gaattcaagc ttgccccatc cgcggcaaaa cgtagggatc cccccgtcgc
     9901 tgttctgcat atccagtagc catttaacac catttcggac tgactccagt aaaggctggc
     9961 tgcgaatgcc aagccgatgc agggtgatca acgcccctgc tgtatcatcg gcatctggca
    10021 cagcccccgg tagattagtc cacccccacc cgccggcgga tgctccggta taaatatgcc
    10081 ggctgcggaa ctgctggtcg agataccatt gggcaatatt cagacgctcg gattcagaca
    10141 acagagtctg ctgtccttcc agcagtgcgc gtactgccaa gctggttacc cacgttgaga
    10201 gattggtatc aatcggccag ctcccgtcat ttcttaccga acggcagaga aaaaccgcag
    10261 ctttttgagc cgttttgctg ccggttaatc ccatcgcagc caaactcatt aggacaaatc
    10321 cggtcagcgg cacagcttct aaaaaacccc catgggtcgg ctgcaggcgc tcgagcaggg
    10381 cctgtgtcgg cgctgttaca aaacggcgaa tgcctcgaac tatgatattg gccgaaggac
    10441 tgaaatgctg tttgacctgc ccgatagcta ttaatgccgg cagcgcatag ctgaccaccg
    10501 tcaggttcag ccatcggaac agacaacggg gcaaaacagc cagttcatac ggcagggctt
    10561 ttacataccg ccagccattt ttgcccagca catctgccaa cgcacacatt gctaaaatcg
    10621 gcactgcaaa agtgcggtcg gtgccgtatg accgataaac cgccttggcc aagctgtctg
    10681 gttccagtcc ccctgccttt tgaaccagcc atcttgccgc agaatcaact gccgttcggt
    10741 atttttcttt gttaatggca ttcaaggcgg cataacacaa caatgtcgag ctgagattgg
    10801 gcggatccag tatctcggca tctccccagc cgccatccgg atgctgatgg gcagccagcc
    10861 agccgagtcc cctgtctatc aattcgctgt gcgctgctgc atcagcacaa tgcagggcaa
    10921 agaccgccac cgcagtcgcc aaagggctgg aactaagccg tccctgccag atgccatcgg
    10981 ctgttcgcgc ctccagcaga agccggcggg cattggacag cattatcgat tcattgtatc
    11041 cctgcacaaa gtattcccgc tgtcagcaga ccataaaaca tgtattctgt atcgctgata
    11101 gaatcgaacg gattggcgca aaatccgacg ccccctttcc agagcatatc cgtaaaacaa
    11161 aggcacggct gccgaatcga ttttaaatct gctcctgctg cggacagtgc aaatacggca
    11221 accgccgtag acagcaaatc cgataccggc gctataggca ttacgcgaaa accgccctcg
    11281 tcatagcagc tcatcagcca ttggacagat ttagaagact ttaccgaacc agttaatgca
    11341 cgctggacaa caatggctgc ggctgttgcc ggaacagaag gagccggaat atcaggctca
    11401 ttgaagtatc cgccctgccc cttattcagc cgctcaagac attctaacat tttgtccctg
    11461 ctgccgtctg gcaaagctgc atccaaatcc tgataagccc ctacagccag aaaacagccg
    11521 taagctgaac cttgattagc cgcatggagg tggtggtaag ccccatccga acagcggaaa
    11581 gattccaacc gtcggcgaaa tgcattctgc gtctgccgat tagaaggcat catcagccgc
    11641 cggcagcgta tcagcgcagc aagatgggct aaatcacaat ccgtacctgt atcagccgta
    11701 ttcaagaagg aaacaacctg tgccctgtta aacggcacct ccagtgcaag cagcgacaaa
    11761 agagcaaaac cggtataata caggtcgctt tgagctcctt tgcctatgaa tcccccatcc
    11821 ttattttgtc ggcaaagaat cacttccgca atctcactct tgcgttcttt cagcagaaat
    11881 cttgagcggg aagccgattc aagaagcgtc tgacaaatat tcatcgcagc attccatcaa
    11941 ttccgtaccg tccagaagtt ttgcagcaac tttgcaaaga aacgccttgc agtcggactg
    12001 gacgatgggt tccagacatt ccacagctgt ttttctgaac tcggcaagca tttttctggc
    12061 ttcgatatcg gcttttgcct gtccttccag ctggagagca gaaagaatag acaatggctg
    12121 gccggcctgt ccggcaagat ggtcataaag gtcatcgcgt atttggtagg caattccgac
    12181 ggcatcgctg taggcatcca gcactttcag cagcggctcg tcagcacctc ccaaaacagc
    12241 ccctattttc agagcaactg caaacgccgg cgaggttttt tgcaaaaata tttccaaaag
    12301 cttctgcctg ccgggagctg tttttgccca gacgcaatcc agctccttcc cttgaccaat
    12361 gcaaagcgtg cggtgtccct gtgcggcaat ttgaagaatg cgcatcctct gggcatcttg
    12421 acaatttaag tcggtcagca attggtagcc cttaccaagc agataatcac ctgcattcag
    12481 cgccgacggt atgccatacc gctgatgcag agtgggctgg ttgtaccgca tcgcatcgtt
    12541 atcttctata tcatcgtgaa tcagcgaggc cttatgaaaa cattcgaccg caatagccgc
    12601 ctgcttgacc gccggcggta tctgctcaac tgccgtcccc gtcagcaccg catagatgcc
    12661 tgccgtcagc atcggccggt atcgcttgcc gtcttcggcc atccaagcca atgcaagcgc
    12721 cgcggccgga ccatctgctt cattaaagac agcccggaga gtatctgcat caaaccagtc
    12781 ccggaccttc tgacccagct gcgacaaatt aaccggtcct gcct
//
LOCUS       DAOTBO010000080        12811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4117_length_12811_cov_5.716761, whole genome shotgun sequence.
ACCESSION   DAOTBO010000080 DAOTBO010000000
VERSION     DAOTBO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4117_length_12811_cov_5.716761"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="PLE88_09370"
     CDS             <1..1826
                     /locus_tag="PLE88_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOM76712.1"
                     /translation="CLCLLKVLDNPCRDIELAALLRSPLFGLTDAQLALIRLHAEKKM
                     SFYTALRKYAADGADAALRSTLSAILEQISDWRRQVRQGSLADLLDGIMRSKGLETFY
                     AAMPNGAQRIANLQKLHDYAVRFEHFRTTEPGTALVRFVEFLEKLAEAEQDWAPAEPD
                     SSAENAVRIMSIHKAKGLEFPVVFVAELNAKFNMQDTAGECLIDEQMLGLQIPVRPAA
                     GRFPSMAHQVIAEQKRRSGLAEEMRILYVALTRAREKLILTASAKSDACTCQLSECSV
                     FDSLPDWKLAEACCPLDWITAGFARQRALASLYYIDHPRLADDNLFYAQRIRRKDLNQ
                     MTEEILQAKRSVPSLCRPPKAGSEADRQAAAAFEAIGRNLRWRYPFEDLTCLPAKLSV
                     SQLSHRDDEFAAGGMQEAFLNKPAGQPAAAAPGGAVLGSAVHRILEHIDMSKPLDVAA
                     VKTTAESLCRAGLLDPSAADAIDPAAICAFFSSRLGMLARAAGAALLREWPFTYALDA
                     AAVGAQSQGEFVILQGIIDMIIPVAEGLVIVDFKTDRIEQMHLSDRAARYAPQVRLYA
                     QAAEAILKRPVVSAWLYFLTPATAVPVELNTAEPLRENPSA"
     gene            complement(1848..2249)
                     /locus_tag="PLE88_09375"
     CDS             complement(1848..2249)
                     /locus_tag="PLE88_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM76713.1"
                     /translation="MEQKAEKQPRVLVIEDDPGHQRLLELYIQRAGCQCDCCFDGRTG
                     LDKALSHEYDAIFIDIHIPEMDGFMVATQIREQGLTVPLIATTALTVEGIRRNASKVG
                     YTEFLQKPLGEKEICDILQRYAFGKPLAQKS"
     gene            3397..3816
                     /locus_tag="PLE88_09380"
     CDS             3397..3816
                     /locus_tag="PLE88_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76714.1"
                     /translation="MKSGRYRGLLAITGIASAGLLLSSTMLLGSQQSDYRRRPIKQEF
                     TIPPAQTEMTLMIEAYERLINQYAGLAESAQRQLQADVDGIGRKLEAIDRKISLLSAQ
                     VETIQKKLGIEPLKQQQTLPVSALQEPANTEAPAENH"
     gene            3823..4632
                     /locus_tag="PLE88_09385"
     CDS             3823..4632
                     /locus_tag="PLE88_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOM76715.1"
                     /translation="MNESVLLLCDKPASDENVRGGDFRFIKAELPVAETQVPLTIKLL
                     PKPISLPDIVPLARAISDKLCSVLLSSLAAGGHSVPCSKGCCVCCRYLVSLSMPEVYR
                     LRQELARMESWYRMPILSSCLSAARKILGSASVMKAKDAKGLSQWYWQLDIECPFLHH
                     GVCSIYAIRPLACREHFVTTPPMLCRPAGLSEPSVVSIPVSILEALGQLAAELEHREI
                     ESVMLPFVFADVDEDNVRCRRTWPADYMVERFVQILQTLASGQSADVLRPR"
     gene            complement(4663..4938)
                     /locus_tag="PLE88_09390"
     CDS             complement(4663..4938)
                     /locus_tag="PLE88_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76716.1"
                     /translation="MIEKIYNNQIQNIAKEFCTPSSGDLSIRPNIQADASLQLNCQSL
                     MEQASLKSADADDGAVVERARQLLLSGQLDTPENIRQAAQNILKFGV"
     gene            complement(5081..5713)
                     /locus_tag="PLE88_09395"
     CDS             complement(5081..5713)
                     /locus_tag="PLE88_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76717.1"
                     /translation="MSKYRLFLILPLLAVFGCGVNEASKSAPTGYYLNPTKNLKALGR
                     TALVELAHHSSAYRVESDVTEALFEEIQKRQIFGLTRIRQDDPAWRSMPIEADGSYTP
                     EEMAWMRKTFKCNAVLTGTITQFEPYPHLTLGLRLRLVDLSDGQLLWAVEQVWDSTDK
                     STLDRIEAYYNPKHLFFDSSNLGGQLGSISSLKFFKFVAHEVAMMLPSEF"
     gene            complement(5722..6279)
                     /locus_tag="PLE88_09400"
     CDS             complement(5722..6279)
                     /locus_tag="PLE88_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76718.1"
                     /translation="MKTKQLLMPSILSGIIVLAGCVQPGQPLESAVKPAQTEMKTDGM
                     AAKRFQEPQTEGPTVIESAIELSKKYAALSEEMTKLQLEKQALAAENSRLKEQVAALE
                     PQLAQTQKELSEANDLLVEMRIELNNWKADVLGFREEMRQADKAQLEALIKILEILNP
                     QGTEAEPNSASVNPKQETAKHPAAE"
     gene            complement(6335..6562)
                     /locus_tag="PLE88_09405"
     CDS             complement(6335..6562)
                     /locus_tag="PLE88_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76719.1"
                     /translation="MQKTAASIVKANDVIVEGRFCLNINQPGPESESSHRSVLPQVRL
                     LEKNTDYAVIEITCGCGSKIRLRCDYDATTA"
     gene            complement(6592..6825)
                     /locus_tag="PLE88_09410"
     CDS             complement(6592..6825)
                     /locus_tag="PLE88_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76720.1"
                     /translation="MTENDYRIVQPLQNFPAVAGLHRIDGNTAREKRQKTPSESKPKK
                     DAAESTPDGQLSPSGSKDCSGTDSNGHFIDIRA"
     gene            complement(6812..7588)
                     /locus_tag="PLE88_09415"
     CDS             complement(6812..7588)
                     /locus_tag="PLE88_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOM76721.1"
                     /translation="MTQPPAAEQVCLKAAACNAYRRQNHPALSDETIAQYLPLVHKVV
                     QRVAVYLKPPLSREDLVSAGLVGLVKAARDYNPSHEAEFQTYAYIRIRSAVLDELKRQ
                     SFVPAVVNKQIRQAAQLRLDLTDQHGQAPSDEELAGQLHISVDKLYQIFSSARIRQFV
                     SYDHFADDESGPADRLAASAPDPSAQMEKTELVDQLAAAISQLDQRLRQIIILYYQQD
                     LTMKQIAQVLGLTEPRVSQLHAKALFLLSSKMRAWKYDRE"
     gene            complement(7593..7838)
                     /locus_tag="PLE88_09420"
     CDS             complement(7593..7838)
                     /locus_tag="PLE88_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76722.1"
                     /translation="MPLDVRSISIRFAAAAFFILSFIGWFAELPPLTCCKRAGAGALI
                     IYITTAAAVNLVNRMLIGLWIKSQSDEHNGDLDGRGF"
     gene            complement(7845..9980)
                     /gene="flhA"
                     /locus_tag="PLE88_09425"
     CDS             complement(7845..9980)
                     /gene="flhA"
                     /locus_tag="PLE88_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOM76723.1"
                     /translation="MTPSGKTSTPSLGMTQTAASSRSNIVFAVGLVVILATLLLRMPT
                     PLLDMLLACSISLSIAVLLVTISSHEPLELSAFPSLLLLVTMFRLSLNVASTRLILTQ
                     GDAGKIIQTFGDFVAGGSFVVGLVIFVILFIIQFIVVTKGAERISEVSARFTLDAMPG
                     KQMAVDADLNAGAITEAQANERRAKIVKESEFYGAMDGASKFIRGDAKAGIIITLINI
                     LGGIATGCARGMSISDAIRTYSVLSIGDGLVSQIPSMIISISSGFLVTKISSSRSVGQ
                     DLSRQFLKNSQPLTVASLVIAALGFVPGLPMLPFFLLAGATALTGRMMASSSAVQETK
                     HAAEQKPAKEQKQPLEELLDVDKVSVHVGVRLIGMVDPRKDSAIFDRIGALRRRFAQQ
                     MGIIMPLVRIKDSISLEPNAYEIRIFDHIVARGKLQPEMYLAMDSGTVQDKIDGLETT
                     EPVYGLPALWIAESQRQTAELNGYTVIDPESVFITHLSETLKKHAAELLTRDDVQQLV
                     DRLRKTQPSLVGDVVGDLVPIGILQRVLKNLLAGGIPIRELPIILEALAEAAVKTKNT
                     DMLTEMVRKHLSRTICEQYKNDKGRIPALTLEPALEHQLTASLRQEGDTLVLGLRAET
                     AMQLTHRIAQAWKAAADKGVERPVLLCDSRLRYSLSAMLSRAVPLLGVLAYDEIVLGT
                     EIEPVQTVSLPKEGQLETDEPVLAAQGKG"
     gene            complement(10008..10424)
                     /locus_tag="PLE88_09430"
     CDS             complement(10008..10424)
                     /locus_tag="PLE88_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOM76724.1"
                     /translation="MSETAVMAEEKKAEQPEDALQAEQSVSPISVQSVEFTNVEEKDA
                     AGTPASLDILLDMEVPITVAIGQADISIQRLLQLGPGSVVKLDKTIDAPAELYLKDTL
                     FALGDIVAADGQFAVRIRDIIGPRNPSGKSEKTKKS"
     gene            complement(10454..11404)
                     /locus_tag="PLE88_09435"
     CDS             complement(10454..11404)
                     /locus_tag="PLE88_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOM76725.1"
                     /translation="MSKLSVDTLSSFKIRQLLSAVGSKGSPQTENQPAVSAYDWRQPR
                     LSTAQRAKLEAFADTVAAECTKEFIRFYPCTMRVSLADVRLCLPDALKAPNPEDTSAE
                     YRIGFGPDAGQLCGWICIPMPSALLWTSRILGDKGQIEDAGRKLSGLEESLLLDMAGG
                     LIQAFSQAYGKNLVSHACFSSGPTAAQKEPDALCQIVLDVQQPDHPNETASFYFVIGC
                     DKVRAVTGGSEERKTAAPAAAMMREHICNLPVTVTVRLADASLCLKDIMNLEVNDVVL
                     LGKKTAEPVELLWGDKILFYGLPAQTGQHRAVVIIDKNSK"
     gene            complement(11401..11967)
                     /locus_tag="PLE88_09440"
     CDS             complement(11401..11967)
                     /locus_tag="PLE88_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOM76726.1"
                     /translation="MADNTAKQDLEQADGNESAGQPAVQIGLLMWLIMITIIVLLSSS
                     GFVLGRLLARSGPSEAAAPAAGDSPAGTQDAAGSRQDTWFFNELESVVVNPNEPGATR
                     YVRVGLILEISSSMSQDKAKALIESKKPLLINWLNLYFKGLSLSEMETDKDISRILSQ
                     ICDGFNEILWPDGKPQIKKILIREFNIQ"
     gene            complement(12013..>12811)
                     /locus_tag="PLE88_09445"
     CDS             complement(12013..>12811)
                     /locus_tag="PLE88_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM76727.1"
                     /translation="VNCAALSDTLLESELFGHEKGAFTGAYTQRKGRFEMAHGGTLLL
                     DEITETPLKFQAALLRVLEQQDFERVGGSNRVRVNVRIISTTNRDLAEETAQGRFRRD
                     LYYRLCGMRLVVPPLRQRLEDLPDLIWFFVNLYSHQTSRRISRLSEDMMNLFRTYHWP
                     GNVRQLRNVVRTSLLLGSGPVLSLADASWMLDDRQPRSQKDYGIIEESPADLGGIALS
                     DLERQAILATLEKTDGNQSKAAKILGISDRTLRDKIRRYQQQECLCR"
BASE COUNT         2965 a         3401 c         3299 g         3146 t
ORIGIN      
        1 actgcctatg cctgctgaaa gttctcgata atccctgccg ggatatcgaa ttggcagccc
       61 tgctgcgcag cccgctgttc ggcctgaccg atgcccagct ggctcttatc cggctgcatg
      121 cagaaaagaa aatgtccttt tacacggcgc tgcggaagta tgcggcagac ggagcggatg
      181 cagcgctgcg cagcacgctg tcggcgattc tggagcagat cagcgattgg agacggcagg
      241 tgcggcaggg atcgctggcc gatctgctgg atggaatcat gcgttccaaa gggctggaaa
      301 cattttatgc ggcgatgccc aacggtgctc agcgaatagc caatctccag aaactgcacg
      361 actacgccgt tcgattcgag catttccgca ccaccgaacc gggcacagcg ctggtgcgct
      421 ttgtggagtt tttggaaaaa ttagccgagg ctgagcagga ctgggctccg gccgaacccg
      481 acagctcggc tgaaaatgcc gtgcggatta tgagcataca caaggccaag gggctggagt
      541 ttcctgtcgt gtttgtcgcc gaactgaatg cgaagtttaa tatgcaggac accgccggcg
      601 agtgtctcat tgatgaacag atgctcggcc ttcaaatacc ggtccggccg gcggctggac
      661 ggtttccctc gatggcgcat caggttattg ccgagcaaaa gcggcgctcg ggtctggccg
      721 aagaaatgcg cattctttat gtggccctga cacgggcaag ggaaaaactg atactgaccg
      781 caagcgccaa atccgatgcc tgtacctgcc agctgtctga atgcagcgtg ttcgattccc
      841 tgcccgattg gaagctggcc gaagcatgct gtccgctgga ttggataacc gccggcttcg
      901 ctcgccagcg ggcgctggct tcattgtatt atattgacca tcctcgtctg gcagatgaca
      961 acctgtttta tgcccagcgc atccggcgga aggatctgaa tcaaatgact gaggagattc
     1021 ttcaggccaa gcggtcagtt ccatctctct gccgaccgcc gaaggccggt tcagaagccg
     1081 accggcaggc tgctgccgct tttgaagcca ttggcaggaa tctgcgctgg cgttatccct
     1141 tcgaggattt aacatgtttg ccggccaagc tttccgtcag ccagctgagc catcgggatg
     1201 atgagtttgc cgctgggggc atgcaggagg cgtttctgaa caaacctgcc ggacagcccg
     1261 cggcagccgc ccccggcgga gccgtgttgg gttcggctgt tcatcggatt ttggaacata
     1321 tcgacatgtc caaacccctc gatgtcgctg ctgtaaaaac aacagcagaa tccctgtgcc
     1381 gggcaggact gctggatccg tccgcagcag acgccattga cccggcggcc atttgtgctt
     1441 ttttcagcag ccgactgggc atgctggccc gggcagcagg cgcagcactg ctgcgggaat
     1501 ggccctttac ctatgcgctg gatgcggctg ctgtcggcgc acagagtcag ggagaatttg
     1561 ttattctgca gggcatcatt gatatgatta ttcctgttgc ggaagggctg gtgattgtgg
     1621 atttcaagac ggaccgcatt gaacagatgc atctttctga ccgtgccgcc cgttatgccc
     1681 cgcaggtccg tctctatgca caggccgccg aagccattct caaacgcccc gttgtttcgg
     1741 catggcttta ttttctcaca cctgcaacgg ccgttcccgt tgagttgaac accgcagaac
     1801 cgctccgtga aaatccgtcg gcttgattaa accggcgatt gccggcatca gcttttctgt
     1861 gccaaaggtt tgccgaaggc gtagcgctgc agaatgtcgc agatttcttt ttcgcccagc
     1921 ggcttctgga gaaactccgt atagccgact ttcgaggcat tgcgccgaat gccttctacg
     1981 gttaaggccg tcgtggcgat cagcggaacg gtcagaccct gttcgcgaat ctgtgtggca
     2041 accataaagc cgtccatctc gggaatatgg atatcaatga aaatggcatc gtactcatgg
     2101 gacagcgctt tgtccaatcc cgttcgccca tcgaaacagc aatcgcattg gcagccggca
     2161 cgctggatgt aaagctcaag cagtctctga tgaccgggat cgtcttcaat aacaaggact
     2221 ctcggctgtt tttccgcttt ctgctccata taggcaaatt atacacacag cccagccgtt
     2281 gtgaagaaga aaagacacat tcagatattt tttaacgtgt ttagagaccg caaaactgga
     2341 aatcgcatat cgtcattttt ttttttgggg gggtactgtc tgccgtactg atgccgttgc
     2401 acgaacgggc ttatgtcttt tgcctgcgca gaaacagcag gataaaaatt gaatggcagt
     2461 attgaaaatg cagaaaaata ttcaaacttt ttcgtctgac ggccgatata gtatgtagaa
     2521 agagcatcaa ttgatggaac agtcaaaaat ggctcttaac caataaccca agaggttatg
     2581 ttggcaggaa cttgaaatgc ctgtctcgcg aatgcacaca aggccgatgg taatcagggc
     2641 acgacaagat atccgctaag catggtacga gaagagccat actaatccaa aagggcagag
     2701 ctgtatgctc tgcctttttt attcggccta aaaaaatcgc ccaaaatgac tgcaggggat
     2761 ttgtattcca gacgcttcaa tcaaatgcct tctctatcca gcctgtgaaa aataatcaca
     2821 ccttttatgc ttccagcaag ataggcaaga tagaaaagat aaaatgagat ggaagcctta
     2881 taaaagagag taatcaacaa ctatcaacag ctctggattt ttacgctaaa aatcagtgtt
     2941 gttgaatgtt gtcgacggta tttaccggcc tcaacaggca caccgccgcc tttggaacag
     3001 tatagcggat gccgttatgc aggaattgcc ggccggatag gcagtgcgga ttgccgctgc
     3061 cggacgttat ttgcaaggtt tgagggggaa atcagcagca ccagagacac cggattttca
     3121 gccaaataaa acagaaccgc tgaaatcgcc gatttgcctg aatggtctta atgtggcatc
     3181 aaaacagcct ttttgcgccg cctgcctgcc gctggatatt catttttcaa accgcttgaa
     3241 ttggataaga accatttaag ttttacgata aagtatcgag aataatagta aaggatggtc
     3301 ttactcctcc attttctttt gctttatcgg acgcttttgt tcaacaggga cgtttgttaa
     3361 aacgcatatt atacctcgaa tggaaagggc tggcttgtga aatcaggacg atatagaggt
     3421 cttttggcaa tcactggaat agcatctgca ggcctgctgc tgtcttcgac aatgcttctg
     3481 ggcagccagc agtcggatta cagacgccgt cccatcaagc aggaattcac tattcccccg
     3541 gcgcaaaccg aaatgacatt gatgattgaa gcctatgaac gcctcatcaa tcagtatgcc
     3601 ggcttggcag aatcggctca acggcaattg caggcagatg tggatggcat cggtcgaaaa
     3661 ttagaggcta ttgacagaaa gatttccttg ctttcggcac aggttgaaac gattcagaaa
     3721 aaattgggca tagaaccgct aaagcagcag caaacacttc ctgtttcagc attgcaggag
     3781 ccggcaaaca cggaagcacc ggcagaaaat cactgatctt ctatgaacga atcggtttta
     3841 ttgctgtgtg ataaaccggc atctgatgaa aatgtcaggg gcggtgattt tcggttcatt
     3901 aaagccgaac tgccggtagc cgaaacacag gtgcccctta caatcaaact gctgccaaaa
     3961 ccgatatccc tgccggacat tgtgcctttg gcacgggcca tttcggataa actgtgttcg
     4021 gtacttttat cttctcttgc cgccggcggg cattctgtcc cctgcagcaa gggctgctgt
     4081 gtatgctgcc ggtatctggt ctcattgtca atgccggaag tgtatcggct gcggcaggaa
     4141 ttggcgagga tggagagttg gtatcggatg ccgattttaa gttcgtgtct ttcggcggca
     4201 cgaaagattt tgggctctgc ttctgtgatg aaggctaagg atgccaaggg gctgagccaa
     4261 tggtattggc aattggatat cgaatgccct tttttgcatc acggcgtctg ttccatttat
     4321 gcaatccggc cgctggcctg ccgcgagcat tttgtaacga caccgccaat gctgtgtcgg
     4381 ccggccggtt tgagtgaacc atcggttgta tcaataccgg tgagcatctt agaggcatta
     4441 gggcaattgg ccgcggagct ggaacacagg gaaattgaat ccgttatgct tccttttgtc
     4501 tttgcggatg tggacgaaga caatgtccgc tgcaggcgca catggccagc ggactatatg
     4561 gtggaacgct ttgttcaaat cctacaaacg cttgcatcgg gccagtctgc ggatgttttg
     4621 cggccgcgct gaatgaacat tactgggact gcccccaata gattaaacac caaatttcag
     4681 gatattttgg gctgcctgcc gtatgttttc gggagtatcc aattgaccgg acagcagcag
     4741 ctggcgtgcc cgttccacaa cggctccatc gtcagcatca gctgatttca gagatgcctg
     4801 ttccataagc gattggcaat tcagctgcaa agaggcatcg gcttggatgt tgggccgaat
     4861 cgacagatcg ccggaggaag gggtacaaaa ttccttggcg atattctgaa tctgattgtt
     4921 gtatattttc tcaatcattc tattgcctta ttttttttct aaatgggcat tcatctatct
     4981 tcgctatttc tgatattatt ttcgaggatt ttaccgcaaa cttaaatatt tttttataat
     5041 ttcttaaaga cagtctttta atgtgccgat aaaaaacctc ttaaaattca gatggcagca
     5101 tcatggcaac ttcatgtgcc acaaacttga aaaacttcag cgagctgatc gaccccaatt
     5161 gtccgccgag attgctgctg tcgaaaaaca gatgttttgg attgtaatag gcctcaattc
     5221 tgtccaatgt tgacttatcc gttgaatccc agacctgttc aactgcccat agcaattgcc
     5281 cgtccgaaag gtcaaccaga cgcaatcgaa gcccaagcgt cagatgggga tagggttcga
     5341 attgtgttat ggtgcccgtc agtacggcgt tgcatttgaa ggtttttctc atccatgcca
     5401 tttcttccgg cgtgtagctg ccgtctgctt caatcggcat gctacgccaa gccggatcat
     5461 cttgacgaat ccttgtaaga ccgaaaatct gccgtttttg tatctcctca aaaagcgctt
     5521 cggttacatc cgattcaacc cgataagcgg aagaatgatg ggccagttct accaatgctg
     5581 ttcgccccag tgccttcaga tttttggtcg gattcaggta atagcctgtt ggtgccgact
     5641 tggaggcttc attgaccccg cagccgaaaa cggctaaaag cggcaaaatc agaaataagc
     5701 ggtatttgct catacagtca cctactctgc ggctgggtgt tttgcagttt cctgtttggg
     5761 atttacagaa gccgagttcg gctccgcttc tgtcccttgg ggatttaaga tttcaagtat
     5821 cttaatcagc gcttccagct gcgccttatc ggcctgccgc atctcttccc ggaagccgag
     5881 aacatcggct ttccagttgt tcagttcaat ccgcatttca accagcaggt cattggcctc
     5941 cgacagctct ttttgggttt gtgccagttg cggctccaat gcagccacct gttctttcag
     6001 ccggctgttt tctgcggcta atgcctgttt ttccagctgc agttttgtca tttcctctga
     6061 caaagccgca tattttttag acagttcaat cgcagattcg atgaccgtcg ggccttccgt
     6121 ctgcggttcc tgaaaccgtt tggcagccat accatcggtt ttcatctcgg tttgcgccgg
     6181 cttgactgcg gattcaagcg gctgtcccgg ctggacgcag ccggctaaaa cgatgattcc
     6241 tgacaatatc gacggcatta atagttgttt tgttttcata tagattcctc tggataaatc
     6301 cacgaagcgg cagcgccgca tttctataac agcgtcaggc ggttgtggca tcatagtcac
     6361 accgcaggcg gatctttgaa ccgcatccgc atgtaatttc gattacggca tagtcggtat
     6421 ttttttccag cagccgcacc tgcggcagaa ccgaacggtg tgaactttcg ctttccggtc
     6481 cgggctggtt aatattcaaa cagaaccgcc cttctacgat cacgtcattg gctttcacga
     6541 tgctggctgc tgttttctgc attatacatc tcctgttgtt gtcatgcacg cttaggcccg
     6601 gatgtcgatg aaatgcccat tgctgtcagt gccggaacaa tccttgctgc cgctgggcga
     6661 aagctgtccg tccggcgtag attccgccgc atccttttta ggcttcgact cgctgggagt
     6721 cttctgtctt ttttcccgtg cggtattgcc gtcaatccga tgcaggccgg caacagccgg
     6781 aaaattctgc agcggctgga cgattcgata atcattctcg gtcatatttc cacgccctca
     6841 ttttggatga aagaagaaac agcgcttttg catgaagctg actgacgcgg ggttccgtca
     6901 gcccgaggac ctgtgcaatc tgcttcatcg tcaggtcctg ctggtaatac agaataataa
     6961 tctgccgcag acgttggtca agctggctga tggcggcggc cagctggtca accagttccg
     7021 ttttttccat ttgcgcagaa ggatccggtg cactggcggc caggcggtct gccggtcccg
     7081 actcgtcatc ggcaaaatgg tcatacgaca caaattggcg gatacgggca ctgctgaaaa
     7141 tctgataaag cttatcgaca gaaatatgga gctgccctgc cagttcttca tccgaagggg
     7201 cttggccgtg ctggtcggtc aggtcaaggc ggagctgagc agcctgacga atctgcttat
     7261 tgacaacggc gggaacgaaa gactggcgtt tcagctcatc cagaacggcg ctgcggatgc
     7321 ggatataggc ataggtctga aattcggctt catgggaagg attgtaatcg cgtgcggctt
     7381 tgaccagacc gaccagtccg gccgagacca aatcttcacg gctcagcggc ggcttcagat
     7441 agacagccac gcgctgaacg actttgtgca ccagcggaag atactgcgcg atggtctcat
     7501 cgctcagtgc cggatgattc tgccggcggt aagcgttgca ggcagcggct ttcaggcaga
     7561 cctgttcagc cgccggtggc tgtgtcattg tttcagaatc cacgtccgtc taaatctcca
     7621 ttgtgctcgt ccgactgaga tttaatccac agaccaataa gcatccgatt gaccaaattc
     7681 accgcagctg cagttgtaat atagataatc aatgcccctg ctcccgctcg tttgcagcag
     7741 gtcagcggag gcagttcagc aaaccagccg ataaaggata gaataaaaaa tgccgctgcg
     7801 gcaaaacgga tcgagataga gcgaacatcc aacggcatct taaattatcc ctttccctgc
     7861 gctgccaaaa ccggttcatc cgtttccagc tggccctcct tcggcagaga gacagtctgc
     7921 accggctcaa tttctgtacc caacacaatc tcatcgtagg ccagcacgcc cagcagcggt
     7981 acggcgcggg acagcatcgc cgacaggctg taccgcagcc gcgaatcaca cagcagaaca
     8041 ggccgttcta cgcctttgtc tgccgctgct ttccaggcct gtgcaatccg atgggtcagc
     8101 tgcatcgcag tttccgcccg aagtcccaga accagcgtgt cgccttcttg gcgaagcgag
     8161 gccgtcagct gatgctctaa agccggctcg agcgtaagtg ccggaatccg tcctttgtcg
     8221 tttttatact gctcgcagat ggtccggctc agatgcttgc gcaccatttc agtcagcata
     8281 tcggtatttt tggttttgac agccgcttcg gccagcgctt cgagtatgat cggcagttca
     8341 cggataggaa ttccgccggc cagcagattc tttaaaacac gctggagaat tccaatcggc
     8401 accaaatcgc ctacgacatc cccaaccagt gacggctgtg ttttgcgaag gcggtctacc
     8461 agctgctgga catcatcccg cgtcagcagt tcagcggcat gttttttcaa cgtttcagat
     8521 aagtgggtga tgaaaaccga ttccggatca atcactgtat agccgttcag ttcggcagtt
     8581 tgacgctgcg attcggcaat ccagagagcc ggaagaccat agaccggctc tgttgtttca
     8641 agcccgtcga tcttatcctg aacggtgcct gaatccatcg ctagatacat ctccggctgt
     8701 aattttccac gcgcaacaat atggtcaaaa atgcggattt cataagcatt gggttccaga
     8761 gaaatgctgt ctttgatgcg caccagcggc atgatgattc ccatctgctg ggcgaaccgg
     8821 cggcggaggg caccaatgcg gtcaaaaata gcgctatctt tgcggggatc gaccattcca
     8881 atcagccgaa cccccacatg gaccgacact ttatccacat ccaacagctc ttccagcggc
     8941 tgcttttgct ctttggcggg cttctgttcg gccgcatgtt tcgtctcctg cacggctgag
     9001 gacgatgcca tcatgcggcc ggttagagct gtagccccgg ccagcaggaa aaacggcagc
     9061 atcggaaggc ccggaacaaa tcccagtgcg gctatcacca gagacgccac cgtcagcggc
     9121 tgggagtttt tcaagaattg tctggataaa tcctgaccga cgctgcggct ggaagaaatt
     9181 ttggtcacca aaaagcccga actgatggaa ataatcatcg acgggatttg gctgaccaga
     9241 ccgtcgccga tagacagtac cgaatacgtc cggatcgcat cgctgatgga cattccgcgg
     9301 gcacagccgg ttgcaatgcc cccgaggata ttgataagag taataataat gcccgctttg
     9361 gcgtcgccgc ggatgaactt actggcgccg tccatggcgc cgtaaaattc gctttccttg
     9421 acaattttgg cgcgccgttc attggcctgt gcttcggtga tagcgccggc gttaagatct
     9481 gcatcaacgg ccatctgctt gccgggcatc gcgtcaagcg tgaagcgtgc ggaaacctcg
     9541 ctgatgcgtt cggctccttt ggtcactaca ataaactgaa taataaagag aatgacaaaa
     9601 atcaccagac ccaccacaaa gctgccgcct gcgacaaaat cgccgaatgt ctgaataatt
     9661 tttccggcat ccccctgcgt cagaatcaga cgggtcgaag cgacattcag cgatagccgg
     9721 aacattgtga ccagcagcag cagcgacgga aatgccgaca gttccagcgg ttcatgggac
     9781 gagatggtga caagcagaac agcaatcgac agagaaatgc tgcacgcaag aagcatatcc
     9841 agcagcgggg tgggcatacg cagcagcagg gtggccagaa tcaccacaag cccaaccgca
     9901 aagacgatat tgctgcgcga ggaagcggct gtctgtgtca tccccaagct gggagtgctt
     9961 gttttgccgg aaggggtcat gaaacggatt tctcccgcgg atagcgatta ggattttttg
    10021 gttttttccg attttccaga gggattgcgg gggccgataa tatcccgaat ccgcacggca
    10081 aactgcccat cagccgccac aatatctcca agagcaaaca gcgtatcctt cagatataat
    10141 tccgccggtg catcgatggt cttgtccagc ttcaccaccg atcccggtcc aagctgcaga
    10201 agccgctgaa tggaaatgtc cgcctgcccg atggccacgg tgatggggac ttccatatcc
    10261 agcagaatat ccaaactggc cggcgtgccg gctgcatcct tttcctcaac attggtaaat
    10321 tcaactgact ggacggatat tggagagacg ctctgttctg cctgcagggc atcttccggc
    10381 tgttccgctt ttttttcttc tgccataact gctgtttcag acatagttcg cttcctttat
    10441 agaaagtatg gagttatttc gagttcttat cgattatcac aaccgcccga tgctgccctg
    10501 tttgggcagg cagaccatag aaaaggattt tgtcccccca aagcagttcg accggttctg
    10561 ccgttttttt cccaagcagc accacatcat tgacctccag attcataatg tctttcagac
    10621 acagtgacgc atcagccagc cgaacggtta cggtaaccgg caggttgcag atgtgttcac
    10681 gcatcatcgc cgctgcaggt gcagctgttt ttctctcctc cgaaccgccg gtaacagcac
    10741 ggaccttgtc acagccgatg acaaaataaa acgatgccgt ttcattcgga tggtcgggct
    10801 gctggacatc aagaacaatc tggcagagtg catccggctc tttctgcgca gctgtcggcc
    10861 cggacgaaaa gcatgcatgg gaaactaaat ttttgccata ggcctgtgag aatgcctgaa
    10921 tcagtcctcc tgccatatcc agcagaagcg attcttccaa tcctgacagt ttgcggcccg
    10981 cgtcttctat ttgccctttg tctcccagaa tccgacttgt ccacaataag gcactcggca
    11041 tcggaatgca gatccagccg cacagttgac cggcatccgg cccaaaacca atccggtatt
    11101 cggcggatgt atcctcagga tttggagctt tcaatgcatc cggcaggcac agccggacat
    11161 cggccaagga tacacgcata gtgcacggat aaaaacggat gaactctttt gtacattcag
    11221 cggcaactgt gtctgcaaac gcttccagtt ttgcacgctg ggcggtgctg aggcgcggct
    11281 ggcgccagtc gtaggcagac accgccggct gattttctgt ctgggggctg cccttgcttc
    11341 ccaccgcaga caaaagctgg cggattttga aactgctcaa agtatcaaca gaaagcttgc
    11401 tcattggata ttaaattccc gaatcagaat tttcttaatc tgcggtttcc cgtccggcca
    11461 gagaatttcg ttgaaaccat cgcatatttg cgacaagatg cggctgatat ctttatccgt
    11521 ttccatttcg ctcagcgaca ggcctttgaa atacagattc agccagttga taagcagggg
    11581 cttcttgctt tcaatgaggg ctttggcttt gtcctgactc atactgctgc tgatttccag
    11641 aatcagcccc acccgaacat accgcgtggc acccggttca ttcggattga ccaccaccga
    11701 ctccagttcg ttaaaaaacc acgtgtcttg cctactgccc gccgcatctt gtgtaccggc
    11761 tggactatcg cctgcagcgg gggcagcggc ctccgaggga ccggaccgtg ccagcaagcg
    11821 ccccagcaca aatcccgagc tggacagcag cacaataata gttatcataa tcagccacat
    11881 taatagaccg atttgaaccg caggctgacc agcggattca tttccgtcgg cttgttcaag
    11941 atcttgttta gcggtgttgt ctgccatttg ctgcctttct atttatctgc aaaaaaagat
    12001 tctttgtctg catcagcggc acagacactc ctgctgttga taccggcgaa ttttatcacg
    12061 cagtgttctg tcgctgatgc cgagtatttt agccgctttg gactgattgc cgtctgtttt
    12121 ttccagcgta gccagaatgg cctgccgttc caagtcagac agagcgattc cgcccaaatc
    12181 ggcaggagac tcttctatta tcccgtagtc cttctgagaa cggggctgcc tatcatcgag
    12241 catccacgat gcatccgcca gcgacagtac cggcccgctg cccagcagca gagaggtccg
    12301 tactacattg cgaagctgac ggacatttcc cggccaatgg taagtcctga aaaggttcat
    12361 catatcctcg ctgagccggc tgatgcggcg gctggtctga tgcgaataga gattgacaaa
    12421 aaaccaaatc aaatccggca aatcctccag acgctgccgt agcggaggca ctaccagccg
    12481 catgccgcac agacggtaat acaaatcgcg gcggaatcgt ccctgtgcag tctcctccgc
    12541 cagatcgcga ttggtcgtgc tgataatgcg tacattcaca cggacacggt tgctgccccc
    12601 gacccgctcg aaatcctgct gttccagcac ccgcagcagg gcggcctgaa acttcagcgg
    12661 cgtttcggtg atctcgtcca gcagcagtgt gcccccgtgt gccatttcaa agcgtccctt
    12721 ccgctgggta taagcgccgg taaatgcgcc tttttcgtgt ccgaacagct cgctttccag
    12781 aagtgtatcg ctcaaagcgg cgcagttgac c
//
LOCUS       DAOTBO010000081        12647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4184_length_12647_cov_4.876509, whole genome shotgun sequence.
ACCESSION   DAOTBO010000081 DAOTBO010000000
VERSION     DAOTBO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4184_length_12647_cov_4.876509"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="PLE88_09450"
     CDS             complement(<1..1473)
                     /locus_tag="PLE88_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76728.1"
                     /translation="MLTIQNLFSWLTVAALLYSGVVTALAVEPPHPILSIPGSSNPTL
                     ANAIINNYTETEYVNNFVPRQSPRNNSYGFVPAVVPGDTGWSWSSSTPNLITSTPSGT
                     VFPTTPAYPIYYENVLVTSGKTLAMPYYLRAGSSTAKSMVFELINHNKRNKLRSDLNV
                     LAPTYMASGSTHATRNQAYARRIAVALLDWAAYMPDYVMTAKNSATYINAGPDYILSS
                     DLQRASDHNGLAHEWADDELFAFDAIYDSPALAQLSIEKGFDVRQYITDNLYCNEGDF
                     IVYRVPINVAISSNLSGPYTVLALVARVLNRPDYIDYMDRYLDATVTRKIQRDGTLSE
                     GMGYSIGYINENLDAARNTRNYFLTRPADTPELQAISVRTNTYVNILEYGRAQWQAAS
                     LPNGQLPSFGDTPFKTYFAARNSGTSVLLPAYGSVSMGAGSSSGTAVQLNQCFAGDNN
                     HMRSDTCAYVLWAFGNEVLGNVRYHNGTPGRQFTEQILAYN"
     gene            complement(1506..2909)
                     /locus_tag="PLE88_09455"
     CDS             complement(1506..2909)
                     /locus_tag="PLE88_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM76729.1"
                     /translation="MQTKAMMGIFAFTLVLGTVGCAHSSQKLQTREQIKKIRMEQFNQ
                     QKFGMFIHWGLYALPAGEWKGKTNYGEWIMLQGNIPSSEYEKFAPQFNPVKFNAQEWV
                     ALAKAAGMNYLVITAKHHDGFCMYDSALTEYDIVDATPFKRDPLKELADECRKAGIMF
                     CTYYSIVDWHHPDFPQKYSQIRPEFPAGFHGDPQDNADIKKYAAYMKGQVQELLTKYG
                     ENGIMWFDGGGSFRNYDRKTLLDGEELVKMIHALQPACLINNRLGFGSDYGTPEQSIP
                     ESTLGTAFEVCMTLNRHWGYNKNDHDWKDAQTVIRNLIDVASKGGNYLLNVGPTAEGV
                     IPEESVKILKEAGQWLEVNGQAVYGTTAGPSSANIRMRPAGCMTQKPGKLFLHVFDWP
                     QNRKIFMEGMKGGIVKRIYLLADKNQTPLSYEPYERSLFIDVPAQSPSPYAGVVVIEY
                     DTSQALEENVIEPLTMG"
     gene            complement(2966..4939)
                     /locus_tag="PLE88_09460"
     CDS             complement(2966..4939)
                     /locus_tag="PLE88_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76730.1"
                     /translation="MDNTDNIMGTKCRLRIRQLFLGAAAVLSSVSAYTQVTLAADGPG
                     STYELISSVLGGYPIEVPDCSHPEFGRHITEAWDDILGKYVFVFHIHVTPDNDRCVNF
                     DRQRNEIKTYGPSPAYLKGYYNDIHTYRWKFKLDAGFQPSPNFTHIFQIKAGDGSDSD
                     APIITLTPRYGTPQTMQLIFTPSSGSSGGGTKANPPLEPFKGEWVEAYVRTLYAENGT
                     IEVVLTRIRDGAILMSYTNNNLDMWRGDATFNRPKWGIYRSLNSSQYLRDEEVRFADF
                     CIAKGSDTCPCDERFFPIDPETPFDQEPASGGDIFFEVIPVGGVTILNYQWFEVAGSE
                     DVPVGTNSPMLIIPAAGYSDTGRKFYCRITADKGTFFSRTAQIRSLETALILHLAFDD
                     GTANDSSGSGNHGILNGDLTFVQDPQRGRVINFDGVNGSYIRIPNSPSLNAAAEQITI
                     AMWLYTDTTTVRQLTEKGGTGGSAWYIPPWGIRMESDRTLRLNWGNGLPNPPGPLYSS
                     AIEANRWTHVAMTYDSTLPFNHRKFYINGVLDAASDWTGGAAANTNDLFIGTDFYNNT
                     SRWAYLGRLDDIRIYNTALCAGQIRRIMTGGPHGDIDGDGRVTLADFAYVSAQWLLDG
                     AVVLSADIWPGCGDGRVNIGDLMLMASHYMESK"
     gene            complement(4966..7440)
                     /locus_tag="PLE88_09465"
     CDS             complement(4966..7440)
                     /locus_tag="PLE88_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HOM76731.1"
                     /translation="MPEIKWIVCTACCWIFTAAADSKAAEVYVNSIAQLQTAINNASA
                     GDRIIVANGTYATSAQITVNRIGTAAKPIVIEAATVGGAEITGSYGFALNSPSAHVHI
                     QGFKFTHISGRNSINSGAVFCRFTRNIFECSGDGNYLLVAGDDAQIDRNEFRNKSTVG
                     NMIDVRGTGTQIARRVWIHHNYFHDFSNAGENGAETIRFGLSGLSMSTGEGLIEYNLF
                     VRCRGENELISNKSSGNTYRYNTFLDSAGAQLTLRHGNDCLVYGNYFRGTDGLRIFGD
                     RHQIFSNYFEGNTKGVDMGNGDGEVAEGSDLTCHDRPDDCVVSYNTFINNNVHYQMAA
                     RTDGLGALNITVANNIFQGGGSMASISSTAPYTGVWIGNVRWNTSSAGSMPSSGYTTV
                     NPLLAADTDGVYHLQSDSPVIGTGIGSFPYVTVDMDGQPRDSAPDKGADEFSIAPIAA
                     KLLTVNEVGPFSALFPIDPATPADQQLIIGEHIVFEVVPLPGAVILNYQWFELTVSGD
                     TAIGTNSPVLIISNASKADTGRQFYCRITTDKGVFTSRTAQICSIWTNLVAYLNFDDG
                     TANDLSGRGNHGLLMNGAAIVTDPERGHVLSLDGVDDYVHLGNDASLNLSSTNQATLA
                     AWIKVAVTKNHNAILSKGEWKDAYSLTVKGDTNPPNRLWTGNDTSVFSDDAIPLNVWT
                     HAAVVIDGNLATFYINGQRNGPANQNRGGPIDNTSAGVSIGREQYSGSLPAGRWFFKG
                     LIDDVRIYNKSLTEDQIQQMMTPPLPGDIDGDGRVDMADFAFLSAQWLTDGAFNPSAD
                     IWPMWGDRGVDLYDLLLIVSHFLESQ"
     gene            complement(7543..11688)
                     /locus_tag="PLE88_09470"
     CDS             complement(7543..11688)
                     /locus_tag="PLE88_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOM76732.1"
                     /translation="MSDAEQTKPVRPCRAEDTVLSLLMFLLFLHGAAYSAESMLAYIG
                     SQGRLEYSFYANEGETNADNVLPDFSYCGYKGGGAAIPDVPVVLTLEPQEGDDTQSIQ
                     NAINTVSARTQNADGFRGAVLLKAGRYQVASTLYILASGVVLRGEGQDVLGTVLEAVT
                     PSDYDVISVGSGSASYAAQSGTIRAITTPYVPSGAVSFEISDTSGFSVGDWISVRRTP
                     NQFWIDDLDMAQWGWTPSGYITDHERYITAISGNTITVDVPLVDVYQDKYGGGQVFKL
                     NPISRISLCAVENLRIESCYAGDTDEEHPWDAIKMQYVENSWIRGVTAQYFAYSCVNV
                     SNYARWITVEDCAFLDPKSIITGGRRYSFNIEGTATRVLIQRCYAYDGRHDFVLGSRT
                     RGPNAFVDCYAAHSNADSGPHHRWSTGTLFDNIYSSNSIAVENRQDSGTGHGWSGAQM
                     VFWNCKTPNQKCDAPKGAMNFAIGSKADKCEGSWAPEEPFGWWEHQWQDVYPRSLYFQ
                     QLQDRLGPTAVNNVSSISQCTGNIWDEILAWAGNTRFQPNPLREGTPADRPLLEGLPV
                     IFEAVPADGAVIREYQWYEISGSNYIRAGENSPLLILPEARAADFGRTFFCRVVTDKG
                     PFWSPAVRITNIPGGTNGQLAEDFNGRTANVTIDNLPCNGVLGGIWDTEGESTGNVQT
                     QIAGENTVLLVSGHSSGTLNRGGGISGLTNPIENTETGVLFFRFKANAGTKPLRSYLG
                     IHHYAGSAFLTSPTCQGNAVTAGFGIVSDASSPVFDIITVDHAMTLQTGLLRGQWYNV
                     WIEADNAADTFNIYVTPAEGPDMAVKPPAESDRITPQGGCTFGIAVQSPLTGAMFLVP
                     RAPDDIPLDSMWIDDIYWNGDSGLTTQQSIIPAAPAHLTAVPGEGIIVLDWDDNTEPD
                     FSHYVVYRSTKPDSGFTRLAEGVTESCYIDRDGMDGQTRYYYRVRAENSAGQLSVFSS
                     EVSSISKIALEVPAGLRCSGMDGAVRLLWDANTQPGFAGFILYRSRCISEGYVPIASG
                     LTVCEYLDQNVENNKTYYYVLTAFNDEGEQTAPCLPVEAVPHLPENLALYKPVTASSY
                     YSSARPEYAVDGLVLNYPEIWYSAQDGSDQQPWIRVDLQETYLIDKIRIHHWPNPGTY
                     SRNRDFDLDVLDDEGNLIWSNYDPATGQGELINAGNWMNSPAMIEYILPPFIRGRYIV
                     LTKRSFLAGASASANISELEIFHRFMAPASPTNLSASANSGSIVLSWDMPAEPVDHYI
                     IYRRNEQNEFVPIANAVGQNWFEDSSAEYGTAYFYVVAAADGQGIESGYSEECRICLP
                     LSADFDSSGIVDFSDFIIFAQQWLSNGSSVPSADIAPHEGDGRVDIADLFMFARQWQI
                     GLR"
     gene            complement(11803..>12647)
                     /locus_tag="PLE88_09475"
     CDS             complement(11803..>12647)
                     /locus_tag="PLE88_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM76733.1"
                     /translation="NPSVVYSNAASLGVKRELAHWTLNQTDYDTANQRYLDSVGGYHA
                     SIAVPGNTPVFIDGIVAPASNGAAVFDPNTYGIVSMLNPSEFTGQLTLSVWILWDGTP
                     PSEYGNVILQKGTAYDTWMWSLKLRQSEGNRAGVRFYNNFGYSAQTANLIEANVWTHV
                     CATWDGTTIRVYINGNLASSDDTGTLGPDTLGVMHIASGDNLPGALDEVRIFNTAYDA
                     TAIALDLYYPVSGKPVCVNPGDPILALYDASGDCIVNLADFALLASHWMDCMLVPDCI
                     ERPQ"
BASE COUNT         3155 a         3370 c         3029 g         3093 t
ORIGIN      
        1 attataggcc aagatttgtt cagtaaactg tcggcccggt gtgccgttgt ggtagcgcac
       61 attgcccaaa acttcattac caaaagccca caatacatac gcacaggtat cggaacgcat
      121 gtggttgtta tcgccggcga aacactgatt gagctgtaca gctgtaccgc tgctgctgcc
      181 ggcacccatt gaaaccgaac cgtaggcagg cagcaggacg gaggtgccgc tgttgcgggc
      241 agcaaagtat gtcttaaacg gcgtatcgcc gaatgaggga agctgaccgt tgggcaagct
      301 ggcagcctgc cactgcgccc ggccgtattc gagaatattg acataagtat tagtacggac
      361 actgatggcc tgcaattcgg gggtgtcggc aggccgagtc aaaaaataat tccgtgtgtt
      421 tcgcgcggcg tcgagattct cgttgatgta gccgatggaa taacccatcc cttcactcag
      481 cgtcccgtcc cgctggattt ttcgggtcac agttgcatcc aaataccggt ccatatagtc
      541 gatgtaatcc ggccggttca ggactcgcgc cacaagtgcc agcacagtat agggccccga
      601 cagattgctt gaaatcgcca cattgatcgg cacacggtaa acgatgaaat cgccttcgtt
      661 gcaatacaaa ttatctgtaa tatactgccg gacatcaaag cccttttcta tgctcaattg
      721 cgccagcgcc ggactgtcgt atattgcatc aaaggcaaac aattcatcat ccgcccattc
      781 gtgggccagc ccgttatggt cactggcccg ctgtaaatcg ctggacagga tatagtccgg
      841 cccggcgtta atataagttg cgctgttctt ggctgtcata acataatccg gcatatatgc
      901 agcccaatcc agcagtgcaa cagcgatgcg gcgggcgtag gcttggttgc gcgtcgcatg
      961 ggtgctgccg ctggccatgt acgtgggggc taaaacattc aggtcgctcc gcagtttgtt
     1021 gcgtttattg tgattgatca gttcaaacac catcgacttg gccgtcgaac tgccggctcg
     1081 aagataatac ggcatcgcca gcgtcttgcc gctggttacc agcacatttt cgtaataaat
     1141 tggatacgca ggtgtggtcg gaaagaccgt cccgctgggc gtactggtaa tcaggttggg
     1201 tgtcgatgaa ctccatgacc agcccgtatc ccccggaact actgccggca caaagccgta
     1261 actattattc cgaggcgatt ggcgggggac gaagttgttg acatactccg tttcggtgta
     1321 attattaata atggcgttgg ccaaagtcgg attggaactg ccggggatac tcaaaatcgg
     1381 atgcggcggt tcaacggcta aagccgttac aactccgctg tacagcagtg ccgcaaccgt
     1441 tagccaacta aataggttct gaatcgtgag cattttttta ctctcgtgta tatttcaaaa
     1501 tacccttatc ccatcgtgag cggctcaata acattctcct ccagcgcttg gctggtgtca
     1561 tattcaatta caacaacacc cgcataagga ctgggcgact gggcaggaac atcaataaac
     1621 aaagatcgct cgtacggttc atagctaagc ggggtctgat ttttatccgc cagcagataa
     1681 attcgcttga caatgccgcc tttcatgccc tccataaata ttttgcggtt ttgcggccag
     1741 tcgaacacat gcaaaaacag cttgccgggc ttttgtgtca tacagccggc tggtctcatg
     1801 cggatattgg cgctgctggg gccggccgtt gtgccgtaaa ctgcttggcc gttaacttcc
     1861 agccactgcc ctgcttcttt cagaatcttg acggattctt caggaataac accttcggcg
     1921 gtcgggccga cattgagcag ataattgcct cccttgctgg ctacatcgat cagatttcga
     1981 attacagtct gggcatcctt ccagtcatgg tcatttttgt tgtatcccca gtgtctgttc
     2041 agggtcatgc aaacctcaaa cgctgtgccc agtgtgcttt cggggatgct ttgttccggc
     2101 gtgccgtagt cagaaccaaa gcccagccgg ttattaatca gacaggcggg ttgcagggca
     2161 tgaatcatct tcaccaattc ctcgccgtca agcagggttt tgcggtcgta attcctgaat
     2221 gaaccacctc cgtcgaacca cataatgccg ttttctccat acttggtcag caattcctga
     2281 acctgtcctt tcatataagc ggcatacttt ttaatgtcgg cgttgtcctg cggatcaccg
     2341 tgaaagccgg cgggaaactc cgggcgaatc tgagaatatt tctgcggaaa gtcgggatgg
     2401 tgccagtcca caattgaata ataggtacaa aacatgattc ccgcctttct gcattcgtcc
     2461 gccaattctt taagcggatc gcgcttgaac ggcgtagcat ccactatatc gtactcggtc
     2521 agggctgaat cgtacatgca gaatccgtca tgatgtttgg cggtaatcac taaataattc
     2581 attcccgcgg ccttggccag cgccacccat tcttgggcat tgaatttgac gggattaaat
     2641 tgcggcgcaa atttttcata ctccgacgag gggatattgc cctgcagcat aatccattcg
     2701 ccataattag tcttgccttt ccattcgccc gccggcagtg catacagccc ccagtggata
     2761 aacatcccga atttctgctg gttaaactgc tccattcgga tcttctttat ctgctcgcgt
     2821 gtctgcagct tctggctgct gtgagcacaa ccgaccgtcc caagaaccaa cgtaaatgcg
     2881 aaaatcccca tcattgcctt tgtctgcatt gttttaaaac ctttctttat tcatcattac
     2941 agaatccatt caggacgcct tgcttttatt ttgactccat ataatggctg gccatcagca
     3001 ttaggtcccc tatattcacc cggccgtcgc cgcagcccgg ccagatatcc gctgacagaa
     3061 caactgcccc gtccagcagc cactgggcac tgacataggc gaaatcagcc agcgtcaccc
     3121 ggccgtcacc atcaatatcc ccatgcggac cgccggtcat aatacgccgg atctgcccgg
     3181 cgcaaagggc agtattataa atgcgaatat catctaaccg cccgaggtat gcccagcggg
     3241 aggtattgtt gtagaaatcc gtcccgataa acaagtcatt ggtattagcc gcagccccgc
     3301 ctgtccagtc gctggcggca tccagtacgc cgttgatata aaacttgcgg tgattgaacg
     3361 ggagtgtcga atcgtatgtc atcgccacat gagtccatcg gttggcttca atcgctgatg
     3421 aatacagcgg tcctgggggg ttgggaagac cattacccca gttcagacgc agggtgcggt
     3481 cgctttccat gcggataccc caaggcggta tgtaccaggc cgaaccgccg gttccgcctt
     3541 tttcagttaa ctgacgcacc gtagttgtgt ccgtataaag ccacattgca atcgtgattt
     3601 gttcggccgc tgcattgagg gaagggctgt tgggaatacg gatgtagctg ccgtttacgc
     3661 catcaaaatt gatgaccctg ccgcgctgag ggtcttgtac aaacgtcaga tccccgttga
     3721 gtatgccgtg gttgccgctg ccggaggaat cattggccgt accgtcatca aaggcaagat
     3781 gcagtatcag ggctgtctcc agtgagcgta tctgtgccgt gcgggagaag aaggttccct
     3841 tgtccgcagt aatccggcag taaaacttcc ggccggtgtc ggaatagccg gcggccggaa
     3901 tgatcagcat cggtgaattc gtaccaacag gcacatcctc cgaaccggca acttcaaacc
     3961 actgataatt cagaatggtt accccgccaa ccggaatgac ctcaaagaaa atgtcacccc
     4021 cgctggccgg ctcctgatca aatggcgttt ccggatcgat aggaaagaat ctctcgtcgc
     4081 agggacatgt atcgctgccc ttggcgatgc aaaagtcggc aaaacgcacc tcttcatcgc
     4141 ggaggtattg cgaactgttc aaactgcggt agatgcccca cttggggcga ttaaaagtcg
     4201 catcgccccg ccacatgtca aggttgttgt tcgtgtaaga catcagtatg gcgccatcgc
     4261 ggatccgcgt gagcacaacc tcgatcgtgc cgttttccgc atacagcgtc cggacatacg
     4321 cctcgaccca ttcaccctta aacggctcaa gcggcggatt ggccttggtg cccccgccgc
     4381 tgctgccgga agagggggta aagataagct gcatcgtttg aggcgttcca taccgggggg
     4441 tcagggtgat aatcggcgca tcgctgtcgg aaccgtcgcc ggctttgatc tggaaaatat
     4501 gcgtaaaatt gggcgagggc tgaaatcctg catcgagttt gaatttccag cggtaggtgt
     4561 gaatgtcatt gtagtagccc ttgaggtagg caggcgaagg tccgtatgtc ttaatctcgt
     4621 tgcgctgccg gtcaaagttg acgcagcggt cattgtcggg cgtcacatgg atatgaaaaa
     4681 caaaaacata cttgcccaga atatcgtccc atgcttccgt aatatgccgt ccgaattccg
     4741 ggtggctgca gtccggaact tctatcggat agccgcccag tacactggaa atcagctcgt
     4801 aagtactgcc ggggccgtcc gctgcaagcg taacctgtgt gtaggccgac accgaagaca
     4861 aaactgcagc tgcgcccaaa aacagctggc gaattcgtaa tctacacttt gtgcccataa
     4921 tattatctgt gttatccatc ggctttttct tgtgccgttt tgccgtcatt gcgattcgag
     4981 aaaatgtgaa acaattaaga gcagatcata caaatctacg cctctgtcgc cccacatcgg
     5041 ccagatatcc gcagaagggt taaatgcccc atcggtcagc cactgggcac taagaaacgc
     5101 aaaatccgcc atatcgactc tcccatcacc gtcaatgtcg ccgggcagag gcggcgtcat
     5161 catctgctga atctggtctt ctgtcagcga cttgttataa atccgaacat cgtcaataag
     5221 ccctttgaaa aaccaccgcc ccgccggtaa cgatccgctg tactgttccc gcccaatgga
     5281 tacgccggca ctggtattgt cgataggtcc gccgcgattc tggttggccg gaccgttgcg
     5341 ctgcccgttg atataaaatg tcgccagatt accgtcgatg accacagcgg catgcgtcca
     5401 aacattcagg ggaattgcat catctgaaaa aaccgacgta tcattgcctg tccatagccg
     5461 attgggcgga ttggtatcac ccttaacggt cagcgaatac gcatccttcc actcgccctt
     5521 gctgagaatg gcattgtgat ttttcgtaac agcaaccttg atccaagcgg ctaaagtcgc
     5581 ctggttggtg ctgctgagat tcagggaagc atcgtttccc aaatgaacgt aatcgtcaac
     5641 gccgtcaagg ctgagcacgt gtccgcgttc gggatcagtt acaatcgctg ccccattcat
     5701 cagcaggccg tgattgcctc tgccggataa atcgttagcc gtgccgtcat caaaattcaa
     5761 ataagcaacc agattcgtcc aaattgaaca aatctgtgcg gtgcgtgatg taaatacgcc
     5821 tttgtccgtc gtgattctac aataaaattg cctgccggtg tcggctttgg aggcatttga
     5881 aataatcaga accggcgaat ttgtcccgat ggctgtatcc cctgatacag tcaattcaaa
     5941 ccactgataa ttcaggataa ccgcacccgg cagagggaca acttcaaaaa caatatgttc
     6001 cccaataatc aattgctggt ctgcaggtgt agcaggatct attggaaaaa gagcagaaaa
     6061 tggtcccact tcattaaccg tcagcagttt cgctgcaatg ggagcaatgg aaaactcatc
     6121 cgcaccttta tccggtgcag aatcccgcgg ctggccgtcc atatcaacag ttacatacgg
     6181 aaaagatccg atacctgttc caatcacagg gctgtcagac tgaagatgat aaacaccgtc
     6241 agtgtctgca gcgagaagcg gatttacagt cgtgtaaccg ctggaaggca tgctccctgc
     6301 actgctcgta ttccagcgaa cattgccgat ccatactccc gtgtaaggag ctgtgctgct
     6361 gatgctggcc atactgccgc cgccttgaaa gatattgttg gctaccgtaa tattcaatgc
     6421 gcccagacca tcggtacgcg cagccatctg ataatgtacg ttgttattga taaatgtatt
     6481 gtacgatacc acgcagtcgt ccggccggtc atggcaggtc aaatctgacc cttcggctac
     6541 ttcgccgtcg ccgtttccca tatcaacgcc tttggtgtta ccctcaaagt aattggaaaa
     6601 tatctggtgg cggtcgccaa atatgcgaag accgtcggtt ccgcggaaat aattgccgta
     6661 caccagacag tcattgccgt gacgcagcgt caattgcgcc cctgctgaat ccagaaaggt
     6721 gttataacgg tatgtattgc cgctggactt attggatatc agttcgttct caccgcggca
     6781 gcgaacaaac agattgtatt caatcaatcc ttcgccggtg gacatgctca gaccgctcag
     6841 gccgaagcga atggtttcgg ctccattttc accggcatta gagaaatcat ggaaatagtt
     6901 atgatgtatc caaacccgcc gtgcaatttg ggtacctgtg ccgcgtacgt ctatcatatt
     6961 gccgacggta cttttatttc ggaattcgtt gcggtcaatt tgagcatcat cgccggctac
     7021 aaggagatag ttgccgtcgc ctgaacattc aaaaatattg cgggtaaaac ggcaaaagac
     7081 cgctccgctg ttaatggagt ttcgcccgct gatatgggta aacttaaatc cctgaatgtg
     7141 cacatgggca gacgggctgt tgagcgcaaa accataactt cctgtaattt ctgcgcctcc
     7201 gacggttgcc gcttcaataa ctatgggctt agcggctgta ccaatccggt taaccgtaat
     7261 ttgagcggat gtggcataag tcccattggc tacaattatc ctatcaccgg cagatgcatt
     7321 gttaattgct gtctgcagct gagcgataga gttcacatat acttctgcgg ctttggaatc
     7381 cgcagctgct gtaaaaatcc aacagcaggc agtgcacaca atccatttaa tctcaggcat
     7441 tatgcctctc tctctttcag catgtctaaa gtcaatttta tcccgacctg ttttgtagtt
     7501 attatccatc tatatgatgc agcttttgct gacacggtca gctcaccgca gtcctatttg
     7561 ccattgccga gcaaacataa aaaggtcggc tatatcaacc cggccgtctc cttcatgcgg
     7621 agcaatatct gcagagggaa cagatgaccc gttagacagc cattgttgag caaaaataat
     7681 aaaatctgaa aagtccacta tcccgctgga atcaaaatcg gccgacaagg gcaggcaaat
     7741 cctgcattct tcggaatacc ccgactcaat gccttgtcca tctgccgctg ctacaacgta
     7801 aaaatatgca gtgccgtatt cggcggatga gtcttcaaac cagttttgtc caactgcgtt
     7861 ggctattggt acaaactcat tctgttcgtt acgccgataa ataatataat ggtccacagg
     7921 ttcggccggc atatcccagc ttagaacgat gctgccgctg tttgccgatg ctgatagatt
     7981 tgtcggtgac gccggagcca taaagcggtg aaatatttca agctctgaaa tatttgctga
     8041 tgccgaagct ccggccaaaa aactgcgttt ggtcaaaaca atatatcggc ctctgatgaa
     8101 gggaggaaga atatactcga tcatcgccgg gctgttcatc cagttgcctg cattaatcag
     8161 ttcaccctga cctgttgcag gatcataatt tgaccagatc agatttcctt catcatccag
     8221 aacatccaaa tcgaaatccc gattgcggct gtatgtcccg ggattaggcc aatgatggat
     8281 tcgaatctta tcaatcagat aagtctcctg taaatcaact cgaatccaag gctgttgatc
     8341 cgatccatcc tgagcggaat accagatttc tggataattc agcaccaaac catccacagc
     8401 atactccggt cgagctgaac tgtaataact gctggccgtt acaggcttat atagagccag
     8461 attctccggc aaatggggta ccgcctcaac cggcagacag ggagctgttt gttcaccttc
     8521 atcgttgaag gccgtcaaga cataataata ggttttgttg ttttcaacat tctggtcaag
     8581 atattcacag acagtcagtc cggaagcaat tggaacataa ccttcactga tgcagcggct
     8641 ccggtaaagt ataaaacccg caaagccggg ctgggtattt gcatcccaca gcagtcgaac
     8701 tgctccgtcc atcccagaac aacggaggcc ggcaggaact tcaagagcga tttttgatat
     8761 actggatact tcggaggaaa agacagacaa ttggccggcg ctgttttcag cccgcacccg
     8821 ataataatag cgtgtctgtc catccattcc atccctatca atataacagc tttcggttac
     8881 cccttcggcc agacgggtga aaccggaatc cggctttgtg gaacgataaa cgacataatg
     8941 tgaaaaatcc ggctccgtat tatcatccca atccaataca attatgcctt cccccggaac
     9001 ggccgttaaa tgggcaggtg cggccggtat gatggattgc tgtgttgtca aaccgctgtc
     9061 gccgttccaa tagatatcat ctatccacat actgtccaat gggatatcat cgggagccct
     9121 cggaaccaga aacattgccc ctgtcagagg cgattgaaca gctataccaa atgtacatcc
     9181 gccttgggga gtaattcgat ccgattccgc aggcggcttt actgccatgt caggaccttc
     9241 tgcgggggta acataaatat taaatgtatc agccgcatta tcggcctcaa tccaaacatt
     9301 ataccactgg ccgcgcagca ggcctgtttg cagcgtcatt gcatgatcga ctgtgatgat
     9361 atcaaataca ggactggaag catcagatac gatgccgaaa cctgcggtaa ctgcattgcc
     9421 ttgacaggtt ggacttgtaa gaaatgcgct gccggcataa tgatgtatcc ctaagtagct
     9481 tcgaagcggt tttgttccgg cgtttgcttt gaagcggaaa aaaagaaccc ccgtttccgt
     9541 attttcaata ggattggtca atccgcttat cccgccgccg cgattcaatg ttccgctgct
     9601 gtgacccgag acaagcagga cggtgttttc accggcaatc tgcgtttgaa cgttgccggt
     9661 actttcgcct tccgtatccc aaatgccgcc caaaacaccg ttacagggca ggttgtcaat
     9721 cgttacgttc gctgtccgac cattaaaatc ttccgctaat tgaccattag ttccgccggg
     9781 aatgttggtg attcgaacag caggcgacca gaacggtcct ttatctgtta caactctgca
     9841 gaaaaacgtt cgtccgaaat cagccgcgcg cgcttcggga agaatcagca gaggactatt
     9901 ctctcctgca cggatatagt tggaaccgga tatttcatac cactgatatt ctctgataac
     9961 tgcaccgtcg gccgggactg cttcaaaaat taccggcaac ccttccaaaa gcggcctgtc
    10021 tgcgggagtt ccttcacgaa ggggattagg ctggaatcgg gtatttcccg cccatgcaag
    10081 aatctcatcc caaatgttgc cggtacactg tgagatggaa gaaacattat ttacagccgt
    10141 cggccccaga cgatcctgaa gctgctgaaa atataaactg cgggggtaaa catcctgcca
    10201 ttgatgctcc caccatccaa atggctcttc cggcgcccag cttccctcgc acttgtccgc
    10261 cttagaaccg atagcaaaat tcatggcccc tttgggcgca tcgcatttct gattgggagt
    10321 tttgcaattc cagaagacca tctgcgctcc cgaccaccca tgcccggtac cgctgtcctg
    10381 gcgattttca acagcgatag aattgctgga ataaatatta tcaaaaagag tcccggtgga
    10441 ccagcggtgg tgagggccgc tgtcggcatt ggaatgggcc gcatagcaat cgacaaaggc
    10501 attcggaccg cgtgttctgg agcccagcac aaaatcatga cgtccatcat aggcatagca
    10561 ccgctgtatt aagacacggg tagccgttcc ttcaatatta aatgagtagc gtcggccgcc
    10621 ggttataatc gatttgggat caagaaaagc acagtcttca acagtaatcc agcgtgcata
    10681 atttgataca ttgacacagg agtaggcaaa atactgagct gtaacgccgc gaatccagct
    10741 gttctcgaca tactgcatct taatcgcatc ccacggatgc tcttcatcgg tatcgccggc
    10801 ataacaggac tctatgcgga gattttctac agcacacaga cttattctgc tgataggatt
    10861 aagcttaaag acttgtcccc cgccgtattt gtcctgatag acatccacta aagggacatc
    10921 tactgttata gtattgcctg aaattgctgt aatgtaccgc tcatgatcag ttatatagcc
    10981 ggaaggagtc catccccatt gcgccatatc caaatcatca atccagaact ggttgggagt
    11041 ccgtcgaact gaaatccaat cgcctacaga aaaaccggat gtatcagata tttcaaaact
    11101 tacagcaccg ctggggacat aaggggtagt aatcgcacgt atcgtaccgc tttgagctgc
    11161 ataactggcg ctgccgctgc cgacagaaat gacatcataa tcggatgggg ttacggcttc
    11221 cagaaccgtc ccgagaacat cctgaccttc accccgaaga accaccccgc ttgccagaat
    11281 atacagcgta gaggccactt gataccggcc agccttaaga agaaccgccc cgcgaaaacc
    11341 gtcagcgttc tgagtacgag cagaaacagt attgatcgca ttttgaatgg actgcgtatc
    11401 gtccccctcc tgcggctcaa gcgtcaacac aaccggcaca tccggaatag ccgcgcctcc
    11461 tcctttgtag ccgcaataag aaaaatccgg caaaacatta tccgcattcg tctcgccttc
    11521 attggcgtaa aaactatatt ccaagcggcc ttgggaacct atatatgcca gcatactttc
    11581 ggcgctgtaa gccgcgccgt gcagaaacag aagaaacatc agcagactca aaacagtatc
    11641 ttctgcacgg caaggccgca caggttttgt ttgttctgca tcactcatat cgcccccata
    11701 tttcttggct tatctgattt tcgcaccaga cccgcaggaa acaggattta tattatgcgg
    11761 gggacgcccg ttatgtcagc gtcccctgct gtcaatttcc acctattgag gccgctcaat
    11821 acaatccggc accagcatac aatccatcca gtgagatgcc agcagtgcaa aatctgccag
    11881 attgacaata cagtccccgc tggcatcata cagagccaaa atcggatcgc cgggatttac
    11941 gcaaaccggc ttgccgctga cggggtaata caggtccaaa gcaatggccg ttgcatcata
    12001 agccgtattg aaaatccgga cctcatccag cgctcccgga agattgtcgc cgctggctat
    12061 atgcatgaca ccaagggtat ccggccccag tgttcccgta tcgtcgctgg aagctaaatt
    12121 tccattgata tagacacgga tcgtcgtacc atcccacgtt gcacagacgt gagtccagac
    12181 attggcttca atcagattag ctgtttgtgc cgaatagccg aaattattat aaaaccgaac
    12241 ccctgccctg ttaccttcgc tttgacgcag tttcagcgac cacatccatg tatcataggc
    12301 agttcctttc tgcaggatga cgttaccgta ttctgaagga ggagtgccgt cccacaaaat
    12361 ccatacactc agcgtcaatt ggccggtaaa ctccgacgga ttcagcatac tgacaatgcc
    12421 gtaggtattg ggatcgaaaa ccgcagctcc attcgaagcg ggagcaacaa taccgtcaat
    12481 aaatacagga gtatttcccg gcactgcaat cgatgcatgg taccccccga ccgaatccaa
    12541 ataccgctgg tttgcggtat cataatcggt ctggttcaat gtccaatgcg ctaattcccg
    12601 tttgacacct aaagaggccg cattggaata gacaacagag ggattat
//
LOCUS       DAOTBO010000082        12624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4197_length_12624_cov_5.314981, whole genome shotgun sequence.
ACCESSION   DAOTBO010000082 DAOTBO010000000
VERSION     DAOTBO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_12624_cov_5.314981"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLE88_09480"
     CDS             complement(<1..803)
                     /locus_tag="PLE88_09480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOM76734.1"
                     /translation="MMENDKPLNIMIVGGNYIGKGAEAMMLVVRDQISRRYPNANFWL
                     IPNSADEAEKLKQDGFKLIYQKKGRRFIKLLQLLCGFLGIQQKTGLSAVESGQSLINP
                     FMATDAVVDISGFVSSDQIGGRAAKGRWLNYMWAAYAGNKIFFMPQSWGPFENKTVRR
                     FTKWLLKKASFICAREKISFRYLIDSGCAKANYVLHSYDIAFLFDVHGSEKRACDLLS
                     LIGLKDSNLPFITITPNMRIYERVQGKDGNNKYIALILEIIRYFLDHTD"
     gene            complement(931..3174)
                     /locus_tag="PLE88_09485"
     CDS             complement(931..3174)
                     /locus_tag="PLE88_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOM76735.1"
                     /translation="MKHYRTYITAAGVCLIAVGAFIVKIASEDGQSAVRPAANAAPLA
                     AAPAAAQPIVDTASKTSAGESFLAAINVQPAQTPLQPVDMPVFYETGWQTYDDYTWTG
                     SPDNVVGYSSSSGSTAYYASAEGGILGSPSSSSSSAAAAAAAETNSGVSGGSEGSSGI
                     SGGSAGGASSSSGDSSSSDTSQTSGDSSADTSSGGTTHTSGSGGAVNPFSNIAVTVVS
                     SMERLLQDDISAPGENYAEIFCAKNETESFQILITNQSYSKLLDVELHVSDWQGPSGA
                     QKPKLTLFREHYVKTTVPSYSLSPDTPIGWYPDALIPFVDPYTGKAITTGLYQANHQS
                     VAPRKSQGYWVDVRVDSDVPAGTYTASILVTSSSRPVAEIPVTLTVWNFALPKQRDWT
                     AWFSMITNLGKVYGLVDNKGDDFELLLQRYQDMLYEHGVYPTMQMPLYPIVDTKTGAV
                     NFDGYNRFLPNFKAFVKRYGPGVYRILDHFPNEFDKYGNNPKLSKTLSDFHHFQTKYP
                     ELGQFFYIIDEPYSDEGGRKVIAVGRIIDRVAPSIKLLVNGAWFIYYKDPSVTEAAVE
                     AAIDIRSIYSLNYLSTPEQLAAVENFISDGKVVWVCQGTRLDRPILHHRIGAWRGYCL
                     GASGQLEWRSSVSDPAMDPWVNPVTYTDDSGKQYNGNGMFIYQGTPNRVGLSGPGGPI
                     ASMRLKVFRDAVDDYAYFKLAEASAGRPAVVSLVDPAAPDFKTSKSPAVYASVRKAVA
                     ALILENQ"
     gene            complement(3340..4737)
                     /locus_tag="PLE88_09490"
     CDS             complement(3340..4737)
                     /locus_tag="PLE88_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76736.1"
                     /translation="MDKRVVAVMAMSVLFSSLAEADLPVFPICTAAGDQVQPDIDGDW
                     IVWHDNRNGTANDDIFGYILSEPNEVPICTAAGRQRYAKVSGSIAVWQDDRDSQRDIY
                     AFDLQNRLPLELSGMPLGDGIQQRYPKISGSYILYEHLVDGLFNLFVYNLSAGTISPV
                     FLSPYRQINSAIEGSIAVWMEDHGSVFQVYSCDVSRSAPATAVSPGSFAQWYPAISGS
                     VVVWAEDRQLGTGLDIYGYDLSEGKEFSICSALGDQNYPDISGRLVVWQDLSKGPSDS
                     DIWAFDLNKGFLFPVAADGQNDQYPAISGRRVVWQRGISGNFDIYMAEIPQPQSIAIL
                     SPSPGQQILAGLSVPVVWQMLEGTLPGEVKIEFSADNGTNWQLIESAVPADEPYQWQT
                     PTVDSQDCLIRVSDPTAPELAAVSGSFTIFRCSPELTADITGDCFVGLDDIAMLASQW
                     LICGNPYNPDWCGNH"
     gene            complement(4852..6177)
                     /locus_tag="PLE88_09495"
     CDS             complement(4852..6177)
                     /locus_tag="PLE88_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOM76737.1"
                     /translation="MAKKPASLEQKIQSKKVVVGVLGLGYVGLPLAREFASAGIRVIG
                     LDIDEPKVRKLNAGKSILKTVSHESVKKMVQSGKFRATADMSEIRKVDAVLICVPTPL
                     TENREPDMQYVEGSCRTIAKYLQKGQLISLESTTYPGTTRELMMPILEATGLKAGRDF
                     YLAYSPEREDPGNKQYCTRTIPKVVGGLTPTCRELARRLYLLAIETMVPVSSLEAAEA
                     AKILENTYRCINIALVNELKMLFDRMGIDVWEVIRAASTKPFGYHPFYPGPGLGGHCI
                     PIDPFYLTWKARQYGMPTRFIELAGEINTDMPHYVVHRVMEALNTRKKSLNGSKVLVL
                     GLAYKPDIDDVRESPSLELIELLKAGGAKVDYNDPYIPQTHKMREYDLKMKSVPLTAA
                     RLKSYDCILISTNHSEYDYKWIVQNARLVVDTRNACAAVKSGRSKIVKA"
     gene            complement(6271..7371)
                     /locus_tag="PLE88_09500"
     CDS             complement(6271..7371)
                     /locus_tag="PLE88_09500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOM76738.1"
                     /translation="MQVPLLDLKAQYAAIKDEVLAKINEVLDSQRCIGGPMVEELEKA
                     IAQVCGCRYAVGVSSGTDALLASLMSLGIGPGDEVITTPFTFFATAGSIARLGAVPVF
                     VDIDPKTFNINPALIEPAITAKTKAIMPVHLFGQMADMDPIMQLAGQTSLAVIEDAAQ
                     AISATYKGKKAGQMGTCGCFSFFPSKNLGGIGDGGMVVTNDEKLHHQLFIMRNHGMEP
                     KYYHHCIGGNFRLDPIQAAALLVKLPHLDDWSNARRQNAAYYNKKFRNSVVQTPFIAE
                     DCVSIYNQYVIRVPRRDALVKHLQSKGIGCEIYYPVPLHLQKCFAYLGKGPGSFPEAE
                     KASREVLALPIYPELTNEMKDYVVQTILDFLS"
     gene            complement(7394..8452)
                     /locus_tag="PLE88_09505"
     CDS             complement(7394..8452)
                     /locus_tag="PLE88_09505"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOM76739.1"
                     /translation="MSEKILVTGTAGFIGFHLARRLLERGDEVVGLDSINDYYDPAIK
                     YARLEQTGIHQQAIEYNKPAVSSTFEKYRFIQLHLEDKPAVEALFARETFGKVCHLAA
                     QAGVRYSLVNPRAYIDSNIIGFFNILELCRQYKIGHLTYASSSSVYGLNESMPFSTSH
                     NVDHPVSLYAASKKSNELMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMALFRFTQAIL
                     EDKPIDVYNYGQMQRDFTYIDDIVEGIVRVLDNPPQGNPHWTGRTPDPSSSKAPYKIY
                     NIGNSAPVKLTDFIEAIEQALGRKANKRMLPLQPGDVPATWADVSDLAENFNYRPATA
                     IQEGISRFVDWYKDFYGV"
     gene            complement(8517..9806)
                     /locus_tag="PLE88_09510"
     CDS             complement(8517..9806)
                     /locus_tag="PLE88_09510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOM76740.1"
                     /translation="MKIAVAGVGYVGLVAAGCLADGGNHVICVDNDAVKIDNLKKGII
                     PIYEPGLSEIVQKNVKAGRLTFTTNLKEGVDNSLIIIIGVGTPSAPDGSADISAVLDV
                     AEKTAQYMTDYRIIVTKSTVPVGTYKKVTDLMASKTNVPFDYVSNPEFLKEGSAVEDF
                     LKPDRVIIGTANPAVMELMKVLYAPFMRKSSRILFMDPASAEMTKYAANVMLASRISF
                     MNELSALCEVYGADIELVRAGIGADSRIGNAFLFAGVGYGGSCFPKDVRALIHMGRQK
                     GVRMGIAEAVNEANYAQHDRFAKKVIDYFAGRNDVQLGIWGLAYKARTDDTRESPAIY
                     CIQKFLDAGMRIIAFDPEASCGPLGDKIQKAADAYDVLAGSEALVIFTDWQEFRSPDF
                     DAIAKKLNRPVIFDGRNLYNPSFVRKIGIEYHSIGRP"
     gene            complement(10164..10967)
                     /locus_tag="PLE88_09515"
     CDS             complement(10164..10967)
                     /locus_tag="PLE88_09515"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOM76741.1"
                     /translation="MHILTRQVRFSIAPFLSDQPTGFNSYASKPAGGDGLSVFLALWV
                     DLEGPVEQQTGFVVNVSTIDKAVRQHAVPVFTRSVQDAFRHCRTLKLEELAGLLKQVW
                     KPIQEVFAAQILRQLRLDLNPYRYLAVQAEDADMLIYSEKFEFSSMHKLWNEAFDAQS
                     NFEHFGKCANPAGHGHNYILEVRVRKKIPQAEQDWVCDFQKVVAEEFLDLVDHKNLNA
                     DVPEFKSLNPTVENLAFLAWEKLEGKFKNVTLCSITVWENDRTYCTYSR"
     gene            complement(10970..11548)
                     /gene="folE"
                     /locus_tag="PLE88_09520"
     CDS             complement(10970..11548)
                     /gene="folE"
                     /locus_tag="PLE88_09520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOM76742.1"
                     /translation="MKEVDLERIERAVLEILYAVGEDPGREGLKDTPRRVAKMYAELL
                     EGLRHEPSEHVKSIFHEQYDEIVLLKDIPFYSICEHHLMPFIGKAHVAYLPDGRVLGV
                     SKLARVVDSFARRLQLQERLTNQIADFLMQNLKPLGVTVVLEAAHSCMTIRGIKKPGA
                     TMITSALRGIFKKDPRSRAEVMTLMHGDKHLG"
     gene            11914..12102
                     /locus_tag="PLE88_09525"
     CDS             11914..12102
                     /locus_tag="PLE88_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76743.1"
                     /translation="MKRLMPTLFGVLFYVSVSFAGYSDGFITAGEYEFGVTWRSNDPP
                     LIVNGGGRTTYPSETTVA"
     gene            12278..>12624
                     /locus_tag="PLE88_09530"
     CDS             12278..>12624
                     /locus_tag="PLE88_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76744.1"
                     /translation="MQYTTKIGADPHIDLYVLLGHSWILDNPLLGVQGQWHDGSPLHI
                     EFINDFDYDPVWTNINFIYPEPATLALLGIGSSLLRKRKKHRPERTCPLLAGFSKSMY
                     IERGFLHCRELLC"
BASE COUNT         3313 a         3246 c         2892 g         3173 t
ORIGIN      
        1 tgatctgtat gatctaaaaa atatcgaatg atttcgagaa taagagcaat atacttattg
       61 ttgccatctt tgccctgaac tctttcatat attctcatat taggcgtgat cgttataaac
      121 ggtaaattac tatccttcag gccaatcagg cttaatagat cacaagccct cttttcagag
      181 ccatgaacat caaatagaaa tgcaatgtca taactgtgta aaacataatt cgctttggca
      241 cagcctgaat ctatcagata gcgaaagcta atcttttccc tggcacaaat aaaactagcc
      301 ttctttagca gccatttggt aaatcgccta acggttttat tttcaaaggg tccccatgac
      361 tgcggcataa aaaaaatctt atttcccgca taagccgccc acatatagtt caaccacctc
      421 ccttttgccg cccgaccccc aatttggtcg ctggatacaa atccagaaat atcaacaact
      481 gcatcggtag ccatgaatgg atttatcaag ctttgaccag attcaacagc cgacaaaccg
      541 gttttttgct gaatacccaa aaaaccacac agcaactgca gaagcttaat aaatcgtctc
      601 cctttttttt gataaattaa tttgaagcca tcttgtttta atttttcagc ctcatcagca
      661 ctattgggga tcagccagaa atttgcgttt ggatatcgac ggctgatctg atcccgaacc
      721 accagcatca tggcttcagc ccctttgcca atataattcc caccgacaat cataatattg
      781 aggggtttat cgttttccat cattcgttat taggttctta gagtgggtta aatatccggt
      841 gtagaggcca gctattgccc ttaataatca ggagcaattt ttcactgttt ttgctccagt
      901 gcatcgacac agcaagtcga aaattaactt ctactgattt tcaagaatca acgccgccac
      961 agcctttcgg acagaggcat acacagccgg gcttttagaa gtcttaaaat caggagccgc
     1021 cggatccacg agcgatacca ccgcaggccg gccagccgat gcctcagcca gcttaaagta
     1081 ggcatagtcg tccactgcat cccgaaagac cttcagccgc atgctggcaa taggtccacc
     1141 aggaccggaa aggccgacgc gattcggtgt tccctgatag ataaacattc cgttgccgtt
     1201 atactgcttg ccggagtcat cggtgtaggt aactggattg acccaggggt ccattgcagg
     1261 atctgatact gacgaccgcc actccaactg gccgcttgcc cccagacaat acccgcgcca
     1321 tgcaccgata cgatgatgaa gaatgggacg atccagtctg gtgccttgac aaacccagac
     1381 cactttacca tcgctgataa agttttccac tgcagcaagc tgttctggcg tggaaagata
     1441 gttaagacta tagatactcc gaatatcaat agccgcttcc accgccgcct cggtaacgga
     1501 tggatcttta taataaataa accatgcccc attgacaagc aatttaattg aaggcgccac
     1561 gcgatcgatt atcctgccga cagcaataac tttacgtcct ccttcatcag aatacggctc
     1621 gtcaataatg taaaagaatt gtcctaattc ggggtatttt gtctgaaaat gatgaaaatc
     1681 agacaatgtt ttagacagct ttggattatt tccatattta tcaaactcat tagggaaatg
     1741 atccaaaatc cgatatacgc ctggaccata tcttttgaca aatgctttaa aattaggcag
     1801 aaatcgattg tagccgtcaa agttaaccgc cccagtcttc gtgtcaacaa tcggatacag
     1861 cggcatctgc attgtcggat aaacaccatg ttcatacagc atatcttgat agcgctgcaa
     1921 cagcaattca aaatcatccc ctttattatc gaccaatcca taaacttttc ccagattagt
     1981 aatcatcgaa aaccatgccg tccagtcgcg ctgtttgggc aatgcaaaat tccaaaccgt
     2041 cagtgttacg gggatttcag ccaccgggcg ggaggaagaa gttaccaaaa tgctggccgt
     2101 gtaagtgccg gcaggcacat ccgaatccac ccgcacatca acccagtagc cctgactttt
     2161 acggggagcc acagactggt gattggcctg atagaggccc gttgtaatcg ctttgcccgt
     2221 gtaaggatcc acaaacggaa tcagagcatc ggggtaccaa ccgataggag tatccggcga
     2281 caagctatag gagggaaccg tcgttttaac ataatgttct cgaaaaagtg ttaatttcgg
     2341 tttttgggct ccagaaggac cctgccaatc tgaaacgtgc agttcaacat caagcagctt
     2401 agaataggat tgattagtaa taagaatctg aaatgattcg gtttcatttt tagcacagaa
     2461 aatctcagca taattttcac caggagcgga aatatcgtcc tgcaatagcc gttccatact
     2521 cgacacaaca gtcacagcaa tattgctgaa cggattaacc gccccgccgc tgccggaggt
     2581 atgcgttgtg ccgccggaag atgtatcagc cgacgaatca ccggacgtct ggctggtatc
     2641 ggaagaagag ctgtcgccgg agctggaaga tgctccgccg gcagaaccgc cggaaatgcc
     2701 ggaagaacct tcgctgcctc cagatacgcc ggaatttgtt tcagcagcag cagcagcagc
     2761 agcagaagaa gaagaagaag aagggctgcc caatataccg ccttctgctg atgcgtagta
     2821 tgccgttgag cccgatgagg atgaatagcc aactacatta tcaggcgagc cggtccacgt
     2881 ataatcatca tacgtctgcc agcctgtttc ataaaaaaca ggcatatcga caggctgaag
     2941 aggtgtctgt gcaggctgaa catttatcgc cgcaagaaaa gactctccag cggaagtttt
     3001 cgatgccgta tctacaatag gctgtgcggc agctggagcg gctgccagcg gtgcagcatt
     3061 agccgcgggc ctaacggccg attggccgtc ttcggatgca attttcacta taaatgcacc
     3121 aactgctatc aaacaaacgc ctgccgccgt gatgtatgtc ctataatgtt tcatcgttta
     3181 taccccgtac tgtgagttta ttgcttcagc tcataaaata taacaagtaa aacaggacgg
     3241 gggctaccgt aatcgcatat ttgagaagtt ttatagtaac tattcactct gccggacaaa
     3301 taacagccct tataaaagag acataatttt accggatatt cagtgattgc cgcaccaatc
     3361 gggattataa ggattgccgc agataagcca ctgggaagca agcatggcaa tatcatccag
     3421 cccgacaaaa caatctccgg taatatccgc cgtcagttct ggactgcatc ggaaaatggt
     3481 aaaggagccg ctgacagcag caagttctgg ggctgtcggg tcgcttacgc gaatcaggca
     3541 atcttgcgaa tcaacagtcg gcgtctgcca ctggtacggc tcatcagccg gcacggctga
     3601 ttcaataagc tgccagttag ttccattatc ggctgaaaac tcgattttga cctcgccggg
     3661 cagcgtccct tccagcatct gccagacaac cggaactgac aaacctgcca aaatctgctg
     3721 gccggggctg ggtgaaagta ttgctattga ctggggctgc ggaatttcag ccatatagat
     3781 atcaaaattg ccggaaattc cgcgctgcca aaccacacgg cggccggaaa tggccgggta
     3841 ttggtcgttt tgtccatcgg cggcaactgg aaataagaac cctttgttca agtcaaatgc
     3901 ccagatatcc gaatcggatg ggcctttgga gagatcctgc cagacaacca atcgaccgct
     3961 tatgtcggga taattttgat cgcccaatgc gctgcagatt gaaaactcct tgccctcgga
     4021 taaatcataa ccatagatat ccaatcctgt gccaagctgt ctgtcttcag cccaaacaac
     4081 cacagatccg cttatggccg gataccattg agcgaaactg ccggggctga ctgctgttgc
     4141 aggagccgag cggctgacat cgcagctgta cacctgaaac acagaaccat ggtcttccat
     4201 ccagactgca atcgaaccct caatagcact gtttatctgc ctgtaagggg ataagaaaac
     4261 cggtgatatt gtgccggctg aaagattata tacaaacaga ttaaagagcc cgtctaccaa
     4321 atgctcatac aggatataac tgccgctgat tttcggatac cgctgctgga ttccatctcc
     4381 taagggcatg ccggaaagct ccagaggcag gcggttttga aggtcaaagg cataaatatc
     4441 acgctggctg tcgcggtcat cctgccaaac tgcaatcgag ccgctgactt tggcataccg
     4501 ctgccgtccg gcggcggtgc agataggcac ttcattgggt tcggacagga tgtagccgaa
     4561 tatgtcatcg ttggctgtgc cgtttcgatt gtcatgccag acaatccagt cgccgtcgat
     4621 atccggctga acctgatcgc cggcagccgt acagattggg aataccggca aatccgcctc
     4681 ggccagtgaa gaaaataaaa cactcatcgc catcaccgcc accaccctct tgtccataca
     4741 tcgtcctcca tttttcatca gaagttattg ctgcagattc cgaacggccg gaatccatac
     4801 ctcgaatgaa aatctttcaa gccctaaagc aaatccgttc aacgccggct tttacgcctt
     4861 gactatcttg ctgcggcccg acttgacagc agcgcaggcg ttgcgggtat ccaccaccag
     4921 acgggcgttt tgaacaatcc atttgtaatc gtattcactg tgattggtgg aaatcagtat
     4981 acagtcatag cttttcagcc gggccgccgt cagcggaaca cttttcattt tcaaatcgta
     5041 ttcccgcatt ttatgcgtct gcggaatata gggatcgttg tagtcaactt tggcgccgcc
     5101 ggctttgagc agttctatca gctccagcga gggcgactcg cggacatcgt cgatatcggg
     5161 tttgtaagcc aatcccaaaa ccaatacttt ggagccgttt aaactctttt tgcgggtgtt
     5221 gagggcttcc atcacccgat ggacaacata atggggcata tcggtattga tttcgccggc
     5281 cagttcaata aaccgcgtcg gcatcccata ttgccgggcc ttccaagtca gatagaacgg
     5341 atctatcggg atgcagtggc cgcctaaacc gggaccggga taaaacggat gatagccgaa
     5401 cggcttggtg ctggcagcac gaataacctc ccagacatcg atacccatcc gatcaaaaag
     5461 cattttcagt tcgttgacca gtgcaatatt gatgcagcgg taggtatttt caagaatttt
     5521 ggcagcttcg gcggcttcca gcgacgacac aggcaccatt gtttcaatcg ccagcagata
     5581 cagccgccgg gccagttccc gacaggtggg cgtcaggccg ccgacgacct tgggaatcgt
     5641 ccgggtgcag tattgcttat tgcccggatc ctcgcgttcg ggggaatagg ccagatagaa
     5701 atcccggccg gcttttaatc ctgtcgcttc cagaatcggc atcatcagct cgcgggttgt
     5761 gccggggtag gttgtgcttt ccagcgaaat cagctgtcct ttctggagat atttggcaat
     5821 cgtgcggcag cttccttcga catactgcat gtcaggctcg cggttttctg tcagcggcgt
     5881 gggaacacaa atcaagacag catccacctt gcgaatctcg gacatatcag cggtagcgcg
     5941 gaatttgccg gattgcacca tctttttgac cgattcgtga gaaacggtct taagaatgct
     6001 tttgcccgca ttgagtttcc tgaccttcgg ctcatcaata tccaatccaa tgacccgaat
     6061 gccggcggat gcaaattccc gtgcaagcgg cagccctaca tagcccaatc caagtactcc
     6121 caccaccacc tttttgcttt gaatcttctg ttccagcgaa gctggctttt tagccatatc
     6181 ttgttctcct aattctgtca gctgtttgat gcagttatag agtttgtatc ggcattttac
     6241 acgtgcagat gttctacgca gtttgattgt ttacgacaga aagtccaaaa tcgtctggac
     6301 aacatagtct ttcatctcat tggtcagttc aggataaatc ggcaaagcca gcacttctcg
     6361 ggacgccttt tcggcttccg gaaaactgcc cggacctttg cccagatagg caaagcactt
     6421 ctgcagatgc agcggcacag gataataaat ctcacagccg atgcctttgg actgcaggtg
     6481 cttgaccaat gcatcccggc ggggcacgcg gatgacatat tggttataaa tcgacacaca
     6541 gtcttcagcg ataaaaggcg tctgcaccac agaattcctg aattttttat tgtagtaggc
     6601 ggcgttttgg cggcgggcat ttgaccagtc atccagatgc ggcagtttga ccagcagcgc
     6661 cgcagcctga ataggatcaa gccgaaaatt gccgccgatg cagtgatggt aatatttggg
     6721 ttccattccg tggttgcgca taataaacag ctgatgatgc agtttttcat cgttggtaac
     6781 aaccatcccc ccgtccccga tgccgccgag atttttgctg ggaaagaaac tgaagcagcc
     6841 gcacgtgccc atctgaccgg cctttttgcc tttgtaggtg gccgagatcg cctgggcagc
     6901 atcttcgata accgccagcg aggtctggcc agcaagctgc ataatcggat ccatatcagc
     6961 catctggccg aacaggtgga ccggcataat ggctttggtt ttagcggtaa tggccggctc
     7021 tatcagggcc ggatttatat taaaggtctt gggatcaata tccacaaaca ccggcacagc
     7081 tccgaggcgg gcaatgctgc cggcggtggc aaaaaaggta aagggggttg taatcacttc
     7141 atcgccggga ccaataccca aactcatcaa gctggccagc agtgcatcgg tgccgctgga
     7201 aacacccact gcatagcggc agccgcacac ctgtgcaatc gccttttcaa gctcctcgac
     7261 catcgggccg ccgatgcagc gctgggaatc gagcacctca ttgatttttg ccaaaacctc
     7321 atccttgatt gccgcatatt gcgcttttaa gtccagcaaa ggtacctgca tgtatcactc
     7381 cgttttctga ttgtcaaaca ccataaaagt ctttatacca gtcgacaaac cggctgatgc
     7441 cttcctgaat ggctgtagca ggcctgtaat tgaagttttc agccaaatcg ctgacatcgg
     7501 cccacgtagc cggcacatca ccgggctgaa gcggaagcat tctcttgttg gcttttcggc
     7561 ccagtgcctg ttctattgcc tctataaaat ccgtcagttt gaccggcgca ctgtttccaa
     7621 tattgtaaat cttataaggc gcttttgaac tggaagggtc cggcgtccgg ccggtccaat
     7681 gcggattgcc ctgcggcgga ttgtcgagca cgcggacaat gccttccaca atatcgtcaa
     7741 tatacgtaaa atcccgctgc atctgtccgt aattgtacac atcaatcggc ttgtcttcca
     7801 aaatcgcctg tgtaaagcga aacagagcca tatccggccg gccccaaggc ccatatactg
     7861 tgaaaaaacg caagcccgtc gtcggcaggc cgaacaggtg gctgtaagta tgagccatca
     7921 gctcgttgga ctttttactg gccgcataca agctgaccgg atggtccaca ttgtggctcg
     7981 tcgaaaacgg catggattca ttcagcccgt aaacgctgga gctgctggca taggtcagat
     8041 gccctatttt gtattgccgg cacagttcaa gaatattaaa aaaaccaata atattggaat
     8101 cgatataggc ccttggattc accaaactgt agcgaacacc tgcctgtgcg gcaaggtgac
     8161 agactttgcc gaatgtttcc cttgcaaaaa gcgcttcaac agccggctta tcttccagat
     8221 gaagctgaat aaaccggtac ttttcaaagg tcgagctgac agcaggttta ttgtattcga
     8281 ttgcctgctg atgaatgccg gtctgctcaa gccgtgcgta cttgatcgcc ggatcataat
     8341 aatcattgat gctgtccagt ccgacgacct catcgccccg ctccagcagg cgtctggcca
     8401 gatgaaagcc gataaatcct gcggtaccgg ttacgagtat tttctcagac atagtaactg
     8461 cttttttctg ctttctgcaa gccggcggct gtcagatgtg ccaaatccga cggcagtcaa
     8521 ggccgaccga tgctgtggta ttcaatacct atctttcgaa caaaagacgg attgtaaaga
     8581 ttccggccgt caaagatgac cggcctgttg agttttttgg ctatggcatc aaaatccgga
     8641 gagcgaaact cctgccaatc ggtaaaaatg acaagggctt ctgagccggc cagcacatcg
     8701 taggcatccg ctgctttttg aatcttgtca cccagcggtc cgcagctggc ttcggggtca
     8761 aaagcgataa tccgcatccc ggcatccaga aacttttgaa tgcagtaaat cgccggggat
     8821 tcacgtgtgt catcggtgcg ggctttgtac gccagccccc agatgcccag ctgcacatcg
     8881 tttcggccgg caaaataatc gataactttt ttagcaaagc ggtcatgctg ggcataattg
     8941 gcctcattga cagcttcagc aatacccatc cggacgcctt tttgccgccc catatgaatg
     9001 agggcacgaa catctttggg aaaacagctg ccgccatagc cgacgccggc aaataaaaag
     9061 gcattgccga tccggctgtc agcgcctatg ccggctcgta ccaattcaat atccgctcca
     9121 tacacctcgc agagcgcact taattcattc ataaatgaaa tgcgtgaagc cagcatcaca
     9181 ttggcggcat atttggtcat ttctgcactg gctgggtcca taaataagat tcggctgctt
     9241 ttacgcataa acggtgcata aaggaccttc atcagctcca taaccgccgg attggctgtt
     9301 ccgataatta cccggtccgg cttgaggaaa tcctctacag ctgagccctc tttaagaaat
     9361 tccggattgc ttacatagtc aaaggggaca ttcgttttgg aagccatcaa atcggtaact
     9421 tttttgtatg taccgactgg gactgtgctt ttggtgacaa tgatacggta gtcggtcata
     9481 tattgagccg tcttttcggc tacatccaat acggctgata tatcggcaga accgtcaggg
     9541 gcgctgggtg tgccaacgcc gataatgata atcagagaat tatctactcc ctctttgaga
     9601 tttgttgtaa atgtcaaacg ccctgccttg acattctttt gaacaatttc agataatccc
     9661 ggttcataga tagggattat acctttttta agattgtcaa tttttacagc gtcgttgtca
     9721 acgcagataa catggttgcc cccgtcggcc agacatccag ctgctaccaa accgacataa
     9781 ccaacgcctg caacagcaat tttcaatagt ttaaatcctt atagataaaa ttcttataaa
     9841 aattcaaaca gcgcaataaa aaacggccaa acagcttaaa acaactagtc caatctacac
     9901 tccgtgcccg tcggttgcgc tgaatgctgg aaaaccgatt ttcagcatca aaataggatt
     9961 atttccgcgt attcacggct tcggaaggca gtcaccctcc caagcaccaa acaccgtgtt
    10021 tcagtaaaaa acatacacgg ttatcatttt tatcggctat tcattacagc tgtattaaca
    10081 agagtttaga tattcaatag aggtttttca taaaatcgat acgccctttt tgccggtcgg
    10141 ttcaattgcc tgcagcgggc aaattaccgg ctgtaagtac aataggtcct gtcattttcc
    10201 cagactgtaa tgctgcatag tgtaacgttc ttaaattttc cttccagttt ttcccaagcc
    10261 aaaaaggcaa gattttcaac ggttggattt aacgatttaa actctggcac atcggcattc
    10321 aggtttttgt ggtccacaag atccagaaat tcttctgcaa caaccttctg aaaatcacag
    10381 acccaatctt gttccgcctg cggtattttt tttctgaccc gaacttcgag aatataatta
    10441 tgcccatgtc cggccggatt ggcacatttt ccaaaatgct caaaattaga ctgggcatca
    10501 aacgcctcat tccataattt gtgcatcgag gaaaactcaa atttttcact gtaaatcagc
    10561 atatcagcat cctccgcctg aacagcaaga tatcgatagg gattcagatc caaacgcagc
    10621 tgcctgagaa tctgtgccgc aaagacctct tgaatcggct tccaaacctg ttttaaaagt
    10681 ccggccagct cttccagctt caaggttctg caatgtctaa acgcatcttg aacagaacga
    10741 gtaaaaacag ggacagcatg ctggcggacc gctttatcga ttgtcgagac atttacgaca
    10801 aaaccagtct gctgctcgac cggaccttcc aaatctaccc acaaagccag aaacacgctc
    10861 aatccatctc cgccggcagg ctttgaggca taggaattaa aacccgtcgg ctggtcggac
    10921 aaaaaagggg ctattgaaaa gcgaacctgt cgggtcagga tatgcatatt tatcctaaat
    10981 gcttgtcccc atgcatcagg gtcattacct ccgcccggct gcgagggtcc tttttgaata
    11041 taccccgcaa tgcgctggtt atcatagtgg caccgggttt tttgatgccg cgaattgtca
    11101 tgcagctgtg agccgcttct aaaactacgg ttactcccaa cggcttgagg ttctgcatca
    11161 gaaaatccgc tatctggttt gtcagacgct cctgaagctg cagccggcgt gcaaacgaat
    11221 cgacaacacg agccaatttg ctgacgccaa gaactcggcc gtccggcaga tacgctacgt
    11281 gggccttgcc gataaacggc atcagatggt gttcacagat actatagaaa ggaatatcct
    11341 taagcagcac aatctcatca tattgttcgt gaaaaatgct tttgacgtgt tcggacggct
    11401 catggcgaag accttccagc aattcagcat acatcttggc aacccttcgg ggggtgtctt
    11461 tgagcccttc ccggccggga tcttctccta cggcatacag aatttccaaa accgctcgct
    11521 ctatccgttc taaatccact tctttcataa atgtcccatt agattgaaaa actagcatta
    11581 tacaacctct aaatccttaa agcaaggaat gacccagcat taccgcagaa aatcgcttta
    11641 agcagctgac cggttaattc tgcatttgcc ggcttataag ccgttgtggc aaataccgct
    11701 ccggcggcta acatccctgc cgatgccaag cagctgaatc cagcagacaa cccatccggc
    11761 aggagccgga acaggaaaat atcgaggatt tttacgaatt aaaagcgttt tagaggactg
    11821 tctggttggc attcgaaccc aaaagtgaaa aatcttgaca taagaagatg ttttcgatag
    11881 aataaacaat aaataacagg cggagtgtct ttgatgaaaa ggttaatgcc gaccctgttt
    11941 ggggttttat tctatgtgtc tgtttccttt gccggttaca gcgatggttt tattacggcg
    12001 ggcgaatatg aatttggtgt tacttggcgg tctaacgacc cgcccctgat tgtcaatggg
    12061 ggggggcgta cgacatatcc gtcagagaca acggtcgcct gattgtgcaa tcgacctcaa
    12121 caccattaca aagatatgtt agcggcgttt atgatattct gcttttaaat ggccatttgc
    12181 tctatttaga ttagatggcc tgaccgaatt ggtcctaata ggcgggagca acgctaccgc
    12241 agttcttcgg ggtggaagta tcaactacat taagagtatg caatatacaa ccaagatagg
    12301 cgccgacccg cacattgacc tttatgttct gctcggccat tcgtggattt tggacaatcc
    12361 cctgctcggc gttcagggac agtggcatga cggctcgcct ttacatattg agttcattaa
    12421 tgacttcgat tacgatcccg tatggaccaa catcaacttc atctaccccg aaccggccac
    12481 cctggccctg ctcggcatag gcagctcgct acttcgaaaa cgcaaaaagc accgcccaga
    12541 aaggacctgc cccctgctgg caggtttttc aaaatccatg tacatagaac ggggctttct
    12601 gcactgtcgg gagttattat gttt
//
LOCUS       DAOTBO010000083        12525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4243_length_12525_cov_5.757658, whole genome shotgun sequence.
ACCESSION   DAOTBO010000083 DAOTBO010000000
VERSION     DAOTBO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_12525_cov_5.757658"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..702)
                     /locus_tag="PLE88_09535"
     CDS             complement(130..702)
                     /locus_tag="PLE88_09535"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOM76745.1"
                     /translation="MLFDGGAVSAALFQWGVVLGASLAAALWDIKSRRIPNVLTLPLL
                     GAGFLQAVMFFGWDGFKSSLFASIVLALPYVLLFLFAGGGAGDAKLMAGIGAWLGLPA
                     AVEALVGISICAVILALLRAFYSRRFFEVLGNIRVMMIAFMFFVGTRGKVQYAGGMET
                     ADDKRALTMPYGPAIFAGLVGTAIYTAIVR"
     gene            complement(726..905)
                     /locus_tag="PLE88_09540"
     CDS             complement(726..905)
                     /locus_tag="PLE88_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76746.1"
                     /translation="MKTLVSFLKDERGLETVEWVVMAALIVLGLVALITGFKSKVAGV
                     FTDVGNEIDAAKNTQ"
     gene            1507..2865
                     /locus_tag="PLE88_09545"
     CDS             1507..2865
                     /locus_tag="PLE88_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOM76747.1"
                     /translation="MQLKHKGIAIVWVAVLLIVFIGFMGLVIDAARVYITGHQLHNAA
                     DAAALAGARWVPDPDSSPLGYNARQKAVEYAAKNYAAQRPVWINENGSFDGQIDFKNS
                     ANIRAILNSGGGVYIGRYIDHSRILYADDDTPDSMLVVVQKKDGNPNGTLPLMFGPIF
                     QVNNIGIQKYAVAKVDDAYGAGLHSITDLEFKGQPIVDVQNGGAIRVGYNVLMSGNAD
                     PLIIAERMYIGEDLDDKYVPPPTLDVQTQLDTTHTDVRFMDPYRLNGPEALIEPTIPP
                     SYLPVSPTSPASSRTVTLKPGYYPNGFNFKGGDNITLESGIYQLGGAGLQISGSAKLN
                     ATSGVMFHIVDTGKVLISTSETGMVNIKGLPVSDGRVIAIFQSRTDTAPAEIQGTGYL
                     NINGEIYFPNNHLEISGSGNGFGTRLIAYSILINGKGSNDAITINFAGRPKIAEKSYL
                     VE"
     gene            complement(2960..5053)
                     /gene="fusA"
                     /locus_tag="PLE88_09550"
     CDS             complement(2960..5053)
                     /gene="fusA"
                     /locus_tag="PLE88_09550"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOM76748.1"
                     /translation="MEKISRIRNIGISAHIDSGKTTLSERILYYAGRIHRMQEVRGEG
                     TGATMDFMDLERERGITITSASTQVIWKDRYINLIDTPGHVDFTVEVERSLRVLDGAI
                     MVLCSVGGVQSQSFTVDQQMKRYRVPRIAFINKMDRTGADPERVCKDMREKLGLNVVP
                     LQIQLGQADNFQGVVDLITMEAVTFEGDNGEKVVRSPVWSDYTEAAQIARQEMLEALS
                     MYNDEMMTLLLEDKPVDEGMIRSTIREATINRDIVPVMMGSAFKNKGVQLLMDAVCDY
                     LPSPLDRCAFARDHDNGGTETPLAGDPDAPLVAMAFKIADETYGQLTYTRIYQGRIEK
                     GKSYRNARTNKMQRIGRIVRMHANDREDISDAGPGDIIALIGVDCASGDTFCGEGINY
                     SLESIYVANPVISLSITPASSADQDRMAKALSRFMKEDPTFRVHSDPETGQTIIAGMG
                     ELHLDIYVERMHREFKANVTVGQPNVSYREAPTIEADFNYKHKKQTGGAGQYAHVVGR
                     LIPLPTDADAPYEFEDNIVSGHIPSEYIPAVNKGFQEAMKKGPLAGYEITGVKMCLND
                     GSFHPVDSSEMAFRICARDAFHEAFKNSKPCLLEPIMKVEVETPAEYQGAIVGDLNSR
                     RGMIMGAEQRGPLTVVRAEVPLASMFGYATVVRGLSKGMATFTMEMCRYAPVPNKLAE
                     EIIFQRRQAQQAARK"
     gene            5578..7236
                     /gene="groL"
                     /locus_tag="PLE88_09555"
     CDS             5578..7236
                     /gene="groL"
                     /locus_tag="PLE88_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOM76749.1"
                     /translation="MAVKDLAFNSEARNALLAGVEKLAAAVKSTLGPRGRCAIIDKGW
                     GGPTVTKDGVTVAEEIELVDKAENMGAKLVREAASKTSKVAGDGTTTATVLAEALFRE
                     AFKNLAAGADAMSLNRGIQKAVAAAVDQLAKLAKPIDIAKKDDIVNIAAISANNDVEI
                     GKKMAEAFMRVGKDGVITIEEGKSLETTVEYVEGMQFDRGYLSPHFVTDADHMTCELN
                     KPYILVYEDKISNVQKIVPLLEAVAKSKRPLLIIAEDVEGEALATLVVNKLRGILQVA
                     AVKAPGYGDRRKAMLEDIAILTGAQPIFKDLGIELDKVTLSQLGQAKKITIDSDNTII
                     VEGAGSSEAIKGRIAQIRSEIETTTSDYDREKLQERLAKLTGGVAQINVGAGSEAEMK
                     EKKARIEDALHATRAAIEEGIVPGGGVALIRCIEAVKKLKLEGDEQTGANIVANALKM
                     PCYYIAENAGAVGTLVVNKVAQGKGGFGYNADKDIYEDLLEAGVIDPHKVTRIALQNA
                     ASVAGLLLTTDCIVTEKPKDKEPAGAGRHGGMGGMGGMDDMDMM"
     gene            7306..7596
                     /gene="groES"
                     /locus_tag="PLE88_09560"
     CDS             7306..7596
                     /gene="groES"
                     /locus_tag="PLE88_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOM76750.1"
                     /translation="MKLVPLEDRVVVKPQEAENKTAGGIVLPDTAKEKPLMGKVIAVG
                     PGKLLDNGSRGSMSVKKNDIVLYGKYSGSEVEIEGTEYKILRESEILGIVEK"
     gene            7641..9257
                     /gene="groL"
                     /locus_tag="PLE88_09565"
     CDS             7641..9257
                     /gene="groL"
                     /locus_tag="PLE88_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOM76751.1"
                     /translation="MAKQMMFQEAARAQLKEGLGQLAAAVKVTLGPTGRNVLLHKSYG
                     SPKVTKDGVSVSKEIELPDPFKNIGAKMVNQVASKTSDVVGDGTTTATVLAEAIYNEG
                     LKYVAAGVNPVALQRGISKAAAVVSDFITASAKPVKGHEDIAKVGAISANNNPAVGEI
                     LADAMDKVGKEGVIEVEEGKGMETELQVVEGMQFDKGYLSPYFMTSAETLECVLEDAY
                     ILLHEKKLSNLRELLPLLEKIAQIGAPLLIIAEDVEGEALAALVINRLQGILKVCAVK
                     APGFGDRRKAMMQDLAIVTGGQLITEDLGIKLESIELSQLGRAKKIVVSKDNTTIIEG
                     AGKKKDITARCDQIRNQIEKTTSNYDKEKLQERLAKLTGGVAVIKAGAATETEMKERK
                     DLLDDALHATKAAAQEGIVPGGGTIYLRAIAEVEKAKAKAKGDEKIGYDIMISALKAP
                     TRQIVENGGGEGDVVVAELLEKSDNIGYDANTGQYVDMIKAGIIDPAKVARTALQNAA
                     SVAGLMLTTNVVIADLKEDKPENAVLGAVI"
     gene            9390..10523
                     /gene="dnaJ"
                     /locus_tag="PLE88_09570"
     CDS             9390..10523
                     /gene="dnaJ"
                     /locus_tag="PLE88_09570"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOM76752.1"
                     /translation="MAKRDYYEVLGVERNASADDIKRAYRRMAIKYHPDKNPGNKEAE
                     EKFKECAEAYEVLSDEEKRKRYDQFGHDGLRGYGVHDYSHMNVSDISDIFGDMFGDVF
                     GDIFGGGRRRSSSGRRASRGYDLETAVELTLEEVARGTEKTIEFTRQDVCPDCSGTGV
                     TKGKQPTRCTMCGGSGQVQRAGLGGFFQMVSTCPQCRGAGEVITDPCKKCRGSGQVPK
                     KRKLNVKIPAGVHEGQGIRVAGEGEPGKMGGPQGDLYCYVRIKSHPFLMRDGSNLVVT
                     VPLSFTQMALGSTIDVPTLDGTRQLKIPAGSQHGSILRIRGQGLPDIRTGVKGDLLVQ
                     LSVVIPKKLTAEQERLLRDFAKTEEQDSETKSFFEKIRQHFNK"
     gene            10526..11128
                     /gene="grpE"
                     /locus_tag="PLE88_09575"
     CDS             10526..11128
                     /gene="grpE"
                     /locus_tag="PLE88_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOM76753.1"
                     /translation="MGEKDLTKADKKIAELEEQIKELNQQIDKLKAEKNETLEQLQRV
                     SADYANYQKRIPRQIAESVLYEKKTFLRSLLPTIDNFERALSHGAASQSQQSLENFLQ
                     GIQLVFEHMLDALKTLGVKRIDAVGQPFDPALHEALMQRCEPDKPDNTILEVCQTGYL
                     LGEQVLRPSKVIVNKFEEKDLPAPDVPQEEAETTDTESEV"
     gene            11130..11432
                     /locus_tag="PLE88_09580"
     CDS             11130..11432
                     /locus_tag="PLE88_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOM76754.1"
                     /translation="MPTYEYKCDYCNYELEAFQSIAAKPLKKCPECGKATLKRLLGTG
                     AGLIFKGSGFYQTDYRSESYKKAAESEKSAASPAKKDSKAESKSENKSTATEASKK"
     gene            complement(11495..12034)
                     /locus_tag="PLE88_09585"
     CDS             complement(11495..12034)
                     /locus_tag="PLE88_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOM76755.1"
                     /translation="MLKPDNTCLILIDIQEKLLRVMHNPEQVVKNCSILIQAAKALGI
                     PIIWCQQAPSALGPTVNELASLLGDFTPIDKTSFSCAGNKNFLDALDALGIQTAVLCG
                     IETHVCVFQTATDLIQKGLYIHVIADATSARTLENKQIGLDRMKSAGAVISSTEMLLF
                     ELLGDSKHEKFRELSKLIK"
     gene            complement(12073..>12525)
                     /locus_tag="PLE88_09590"
     CDS             complement(12073..>12525)
                     /locus_tag="PLE88_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76756.1"
                     /translation="DIGLIRFMVPNRIDDTMTYQITAPAEKIAELVDALQPVWPRCEQ
                     VILNVYNSANKQTIEIPNVQPEQISTLAREDSPELLARLAVRYASANLKRDDSYAGRP
                     AGIENQLSDNIYPELNPPILTGRYAPNQTTPVLEQPVKLHISVKRNAE"
BASE COUNT         3400 a         3023 c         3142 g         2960 t
ORIGIN      
        1 aaaaagccgg acttaaaata tgattcgttc cattagttat tcaaaggtca ataaaagcag
       61 cggcagaaca actgccgttt caacaattgt tgtgccccga ctccttaacg tgctccgtgt
      121 attcatcgtt cacctcacga tagcggtata aatggcagtg ccgacaaggc cggcaaaaat
      181 ggccggaccg tacggcatgg tcaaggccct tttgtcatcg gccgtttcca tgccgccggc
      241 gtattgaacc ttgcctctgg taccgacaaa aaacataaaa gcgatcatca tgacccgaat
      301 attgccgagt acttcaaaga accgccggct ataaaacgcc ctcaacaggg caagaattac
      361 cgcacagatt gaaatcccaa ccagcgcctc aacagcagca ggcagcccca gccaggcacc
      421 gatgccggcc atcagcttgg catcgcccgc gccgccgccg gcaaacagaa acagcagcac
      481 ataaggcagc gccaagacaa tacttgcaaa gagcgaactc ttgaatccat cccatccgaa
      541 aaacatcacc gcctgtaaaa aaccagctcc cagcaaaggc agggttaaca cattgggaat
      601 ccggcggctc ttgatatccc acagtgccgc agccagtgag gcgcctaata cgacgcccca
      661 ctggaataat gctgcactta cagccccgcc atcaaacagc atacaaaaac aaacactaat
      721 aaaaattact gagtgttttt ggctgcatca atttcattgc caacatctgt gaacacgccg
      781 gcaactttgg atttgaaacc tgtgatcagt gctaccaatc ccagaacaat caaggcagcc
      841 atcacaaccc actcgacggt ttccaagccg cgctcatcct tcagaaagct taccagtgtt
      901 ttcattgcat tcccaccttt cttaacaaaa tggagtcctg tttcgtccgt gagattttct
      961 tattcctttg ataggttcac gcaccttttt tatgtacaat tgttagagaa caaaaagtca
     1021 atcacatgca gcgaaatgat cggtgtcaaa caactcctta tttatttttt ttcgttgtcc
     1081 tctcttccat cttgcaggaa tttaactgtg ctgtcggatt tttgtttaac tcccacatag
     1141 tcatcaatta gcttttattg tacagcagaa ggcaaaatat ttcaagaaaa catcggtttg
     1201 ggacgataat ttttaagact gcctatcgtt tcccaagctc cgtccgataa agacgaagca
     1261 tcattgtatt caggttcatt attataggaa aaagagattg ctgtcggtat ttttaccgat
     1321 agcagaaaaa accgtttcca taagaaattt tcttttttat ttggcttaga cacccttctg
     1381 cttgtgattt tccaaaaaat ttggtatgat aaaataatgt ggattcctgc cggcagcaag
     1441 ctgtgcacca atttgcaatg cagcccctgc cggcttccga aatcacttct atgaaggaag
     1501 atagatatgc aactcaaaca caaaggcatc gcaattgtct gggtagcggt tctgctgatt
     1561 gtatttatcg gctttatggg gcttgtgata gatgccgccc gtgtgtatat caccggtcat
     1621 cagctccaca atgccgccga tgcagcggcc ttagccggag caagatgggt tcccgaccct
     1681 gactcttcgc ctcttggata taacgcccga cagaaagccg tagaatatgc cgctaagaat
     1741 tatgcagcac aaagaccagt ctggataaat gaaaacggct cttttgacgg ccagattgat
     1801 tttaaaaatt cagccaacat acgtgcaatt ctcaatagcg gcggcggtgt ttatattggc
     1861 cgctatattg accattcacg catactttat gcggatgatg atacgccgga ttccatgctg
     1921 gtcgtcgtcc aaaaaaaaga cgggaacccc aacggaacac tgccgctgat gttcggcccc
     1981 attttccaag tcaataacat cggcatccag aaatacgccg tcgccaaggt tgatgatgct
     2041 tatggagccg gtttgcattc gattacagat ttagaattta aggggcaacc cattgtagac
     2101 gttcaaaatg gaggagcaat acgggtaggt tacaatgttt taatgagcgg aaatgctgac
     2161 ccgcttatta tagcggaacg catgtatatt ggtgaagacc ttgatgataa atatgttcca
     2221 cctcctactt tggatgttca gacacaattg gatacaactc atacagacgt gaggtttatg
     2281 gatccctatc gactgaacgg tcccgaagcg ttgattgagc cgacaatacc tccgtcttat
     2341 ttacctgttt ccccaacctc tccagcgtca agcaggacag tcacgctaaa acccggttat
     2401 tatccgaacg gttttaattt taagggcggc gataatatta cgctggaaag tggtatttat
     2461 cagttgggcg gtgctggttt acaaatcagc ggaagtgcca aattgaatgc gacaagcgga
     2521 gttatgttcc acattgttga taccggaaaa gttctaatca gcaccagcga aacgggaatg
     2581 gttaacatta agggattgcc ggtaagcgat ggccgcgtta ttgccatctt ccagagtcga
     2641 acagacactg cgcctgcgga aattcagggt accggttatc tcaatataaa cggagaaatc
     2701 tatttcccga ataatcattt ggaaatcagc ggttcaggca atggtttcgg tacacgtttg
     2761 attgcctatt caatcttaat aaatggaaaa ggcagtaacg atgccataac gatcaatttc
     2821 gccggcagac ccaaaatcgc tgaaaagtcc tatcttgtgg aataaaaaac ttggtaacgc
     2881 ggacgtcagc gcccgcaaag aaacacaggc aacctgtctg cgccgtattt taacgcggtg
     2941 tggaagaatc gcgttctttc tatttcctcg cggcctgctg ggcctgtcgc ctctggaaga
     3001 taatttcttc ggccagcttg ttgggaaccg gggcatagcg gcacatttcc atcgtgaatg
     3061 tcgccattcc cttgctcaaa ccccgcacca cagtagcgta tccaaacata ctggccagcg
     3121 gcacctcggc ccgtacgact gttaagggtc ctctttgttc ggcccccata atcatccccc
     3181 tgcggctgtt gaggtcaccg acaatagccc cctgatactc cgcaggggtt tccacttcta
     3241 ctttcataat cggctccagc agacagggtt tggaattttt gaaggcctca tgaaaggcat
     3301 cgcgggcaca aatacgaaag gccatttcac tcgaatcgac agggtgaaac gacccgtcat
     3361 tcaaacacat tttgacaccg gtaatttcat acccggccag aggtcctttt ttcatcgctt
     3421 cctgaaagcc cttattgaca gccgggatat actcgctggg aatgtgcccg ctgacgatat
     3481 tgtcctcaaa ctcataaggt gcatcggcat ctgtcggcag cggaatcagc cgcccaacca
     3541 catgggcata ctgcccggcg ccgccggttt gctttttatg cttgtagtta aagtcggcct
     3601 cgatagtcgg cgcttcacga tagctgacat tcggctggcc aaccgttaca ttggccttga
     3661 attcccgatg catccgctcc acataaatat caaggtgcag ttcccccatt ccggcgataa
     3721 tcgtctggcc tgtttcaggg tcgctgtgga cacggaaggt cgggtcttct ttcataaatc
     3781 ggctcagggc cttggccata cggtcttggt cggctgaact ggcgggagta atcgacagac
     3841 taatgaccgg attggccaca tagatgcttt ccagtgaata attgatgcct tcgccgcaga
     3901 aggtatcgcc gctggcacaa tccaccccga tcagcgcaat aatatcgccc gggccggcat
     3961 cggaaatatc ctcacggtcg ttggcatgca tccgtacaat acggccgatg cgctgcatct
     4021 tgttggtacg ggcattccga taggatttgc ctttttcaat gcgtccctga taaatgcggg
     4081 tataggtgag ctgcccatag gtttcatcgg caatcttaaa tgccatcgca acaagcggcg
     4141 catcgggatc ccctgccaga ggcgtttcgg tgccgccgtt gtcatggtcg cgggcaaagg
     4201 cgcagcggtc cagcgggctt ggcaggtagt cgcagacagc atccatcaga agctggacgc
     4261 ctttgttttt aaacgccgaa cccatcatca ccggaacaat atcgcggttg attgtggcct
     4321 cgcggatggt gcttcgaatc atcccttcat cgacgggctt gtcttccagc agaagggtca
     4381 tcatttcatc gttgtacata ctcagggctt ccagcatttc ttggcgcgca atctgggccg
     4441 cttcggtata gtctgaccag acaggactgc ggacaacctt ttcgccgtta tcgccttcga
     4501 aggttacggc ttccatcgta atcagatcca caacaccctg aaaattatcg gcctgcccca
     4561 attgaatctg cagcgggaca acattaagcc ccagtttttc ccgcatatct ttgcacaccc
     4621 gttcgggatc ggctcccgtg cggtccatct tgttgataaa agcaatgcgc ggcacacggt
     4681 agcgcttcat ctgctggtca accgtgaagg actggctctg tacacccccg acagaacaaa
     4741 gcaccataat cgctccatcc aacacacgca gcgaccgttc tacctcaacg gtaaagtcca
     4801 catgaccggg tgtatcgata agattgatat agcggtcttt ccagataacc tgtgttgagg
     4861 cggaggtaat ggtgatgccg cgctcccgct cgaggtccat aaaatccatt gttgctccgg
     4921 ttccttcgcc gcggacctcc tgcattctgt gaatgcggcc tgcataatac aatatgcgct
     4981 cgctaagggt cgttttgcct gaatcaatat gagccgaaat accaatattt ctgattctgc
     5041 tgattttttc cataaaaact ctgtctttct ctatccactt gctaaatagt caatttaaaa
     5101 aaacatccca cccccatatg caatatggaa tccgaatgct gaagcggcgg gaaatgataa
     5161 tcaaatcata taaagatttt ttattatcct ataaatcaat ataaagtcaa gtaaattttt
     5221 gccgtctgta caattttttc ggtgttttcc atctcttcta ttctatctat gtcatttcta
     5281 tgtcattctt tggatatcct cccggtgttt atcgctctgc cggaatggca gaatatccct
     5341 ggtgttgatt ggttataaat ggcagatgat atagatttga ctgattttcg ggagtctttg
     5401 tcagaatgtt attgaatctg tatcttcttt ctataaaata gcttataaag attttactcg
     5461 gtcttgaatt aagaaagctt ttgtgactgg tattgatttt gcatatttag cgttcttctg
     5521 tggttttaaa aaaagacaat ctaactacat taaacggaaa ttaggagagg caacattatg
     5581 gcagtgaaag acttagcttt taacagcgaa gcccgcaacg ccctattggc cggtgttgaa
     5641 aagctggccg ctgctgtgaa atccacatta ggtccacgcg gccggtgtgc aattattgat
     5701 aagggctggg gcggacctac cgttacgaaa gacggtgtaa ccgtagccga agaaatcgag
     5761 ctggttgaca aggctgaaaa catgggagcc aagcttgtcc gtgaggcagc cagcaaaaca
     5821 tccaaagtcg ccggtgacgg caccaccaca gccactgttc tggccgaagc acttttcaga
     5881 gaggccttta agaatcttgc cgcaggggca gatgcgatga gcttgaatcg cggcatacag
     5941 aaggccgttg cggcagctgt tgaccaatta gcaaaacttg ccaagccgat cgatatcgcc
     6001 aaaaaagatg acattgtcaa tattgctgcc atcagcgcca ataacgatgt cgaaatcggc
     6061 aagaagatgg ccgaggcctt tatgcgggtc ggcaaagacg gcgtgatcac gattgaagag
     6121 ggtaagagtc ttgaaacgac tgtcgagtat gtagagggca tgcagttcga cagaggttat
     6181 ctgtcgccgc actttgtaac agatgcagac catatgacct gcgaactgaa caagccctat
     6241 attctggttt atgaagataa aatcagcaat gtccaaaaga ttgtcccgct gctggaagct
     6301 gtcgccaaga gcaaacgccc ccttctgatt attgccgaag atgtcgaagg cgaggcgctg
     6361 gcgacactgg ttgtcaacaa actgcgcggc attctgcagg ttgcggccgt gaaggctccc
     6421 ggctacggcg accgccgcaa ggccatgctg gaagatattg ccatcttgac tggcgcccag
     6481 ccgattttca aagatttagg gattgagctg gataaagtaa cgctcagcca gcttggtcaa
     6541 gccaaaaaga ttaccattga tagcgacaat acaataattg tggaaggggc tggctcatct
     6601 gaagcaatca agggacggat tgcccagatt cgcagtgaaa tcgaaacaac aacaagcgat
     6661 tacgaccgtg aaaaacttca ggaacggctg gcgaaactaa ccggcggcgt tgcacagatc
     6721 aatgtcggcg ccggcagcga agccgaaatg aaggaaaaga aggcccggat tgaagatgcc
     6781 ctgcatgcca cccgtgcggc tattgaagaa ggcatcgtgc ccggcggcgg cgttgcctta
     6841 atccgctgca ttgaagcggt caagaaactc aaactggaag gcgatgagca gaccggtgcg
     6901 aatattgtag ccaatgccct caagatgccc tgctactata ttgcagaaaa tgccggtgcc
     6961 gtcggaaccc ttgtcgtcaa caaagttgct cagggcaaag gcggctttgg ctacaatgct
     7021 gacaaagaca tctatgaaga ccttctggag gccggtgtta tcgatcctca taaggttacg
     7081 cgcatcgccc tgcagaatgc agcttcggtc gcgggtctgc tgctgactac cgactgcatt
     7141 gttacagaaa aaccgaagga caaagagccc gccggcgccg gccgacatgg cggcatgggc
     7201 ggtatgggcg gtatggatga tatggatatg atgtaaagcc caaacggcag aacactgccg
     7261 aaaacagcaa cataaccgac aatcaattaa acaggagaga catatatgaa actggtacca
     7321 ttggaagatc gcgtggtcgt caagccgcag gaagcagaaa acaaaaccgc cggcggaatc
     7381 gttctgccgg acacagccaa agaaaaaccg ctgatgggca aggtgattgc tgtcggcccc
     7441 ggcaagcttc tggacaacgg cagccgcggc agtatgagtg tcaagaagaa tgatatcgtg
     7501 ctctacggca aatacagcgg ctcggaagtc gagatagaag ggacggaata caagattctt
     7561 cgcgaaagcg agattctggg tatcgtcgaa aaataactga atttcaacta tccctaagat
     7621 agtatggagg ttatcaaaca atggcaaaac aaatgatgtt tcaggaagcg gcccgggccc
     7681 agctcaaaga agggctgggg cagctcgctg ctgccgtcaa agtaaccctc ggcccgacag
     7741 gccgcaatgt gctgctgcac aaaagctacg gttcccccaa ggtcacaaag gacggggtgt
     7801 cggtcagcaa ggaaatagag ctgccggacc cgttcaagaa tatcggtgcc aaaatggtca
     7861 atcaggtggc cagtaaaacc agtgatgttg tcggcgatgg aacaacaacc gctacggttc
     7921 tggcggaagc aatttacaat gaagggctta aatatgttgc tgccggtgtc aatcccgttg
     7981 cccttcagcg gggcatcagc aaagcggcgg cggtggtaag cgattttatc actgcttctg
     8041 ccaaacccgt caaggggcat gaagatattg ccaaagtcgg tgccatcagc gccaacaaca
     8101 atccggcggt cggggagatt ttggctgatg ctatggataa ggtgggcaag gaaggcgtca
     8161 ttgaagtcga agaaggcaag ggaatggaaa cagaactgca ggttgttgaa ggcatgcagt
     8221 ttgataaagg ctatctgtcg ccttatttta tgaccagtgc cgaaacgctc gagtgtgttc
     8281 tcgaagacgc ctatattctg ctgcatgaaa agaaactttc caacctgcgg gaactgctgc
     8341 cgctgctgga aaaaattgct caaatcggcg ctcccctgct gattattgca gaagatgtag
     8401 agggtgaggc gctggcggca cttgtaatca accggctgca gggcatccta aaggtttgcg
     8461 ctgtcaaagc tcccggattt ggcgaccgcc gcaaagccat gatgcaggat ttggccattg
     8521 taaccggcgg tcagctgatt accgaagatt tgggcattaa gctggaaagc atagaattgt
     8581 cgcaattggg ccgcgccaag aagatcgtgg tcagcaaaga caacaccaca ataatcgaag
     8641 gtgccggcaa gaagaaagac atcacagccc gctgcgacca gattcgcaat cagattgaaa
     8701 aaacgaccag caactatgac aaagagaaac ttcaggaacg ccttgcgaaa ctgaccggcg
     8761 gggtggcagt tattaaggcc ggagccgcaa ccgaaaccga aatgaaagaa cgcaaagatc
     8821 tgctggacga cgccctgcat gctaccaagg cagcagcgca ggaaggtatc gtccccggcg
     8881 gcggaacaat ctatctgagg gctatcgcgg aggttgaaaa agccaaagct aaagccaaag
     8941 gcgatgaaaa gattggatac gatattatga ttagcgccct gaaagccccg actcgccaaa
     9001 tcgtcgaaaa cggcggcggc gaaggcgatg tggttgtggc tgaactgctc gaaaaaagcg
     9061 acaatatcgg ctatgacgcc aataccggcc aatatgtgga tatgatcaag gccggcatca
     9121 tcgatccagc caaggttgct cgtaccgccc tgcagaatgc agcctctgtt gccggattga
     9181 tgctgacaac caatgttgtc attgcagatt tgaaagaaga taagcccgaa aatgccgttc
     9241 ttggtgcggt aatttaagcg gcgattttta ttgccgggtc ccgcctgggg ttgggggcag
     9301 agcacagacg ggactcggct ttgttcttta taaaacacgt atccgaatta gacagttcgg
     9361 tgtttatata tattggatca agatagctta tggccaaacg cgattattat gaggtgcttg
     9421 gggttgagcg aaacgcatca gccgatgata ttaaacgcgc ctatcggcgg atggccatca
     9481 aataccatcc tgacaaaaat cccggcaaca aagaagccga agaaaaattc aaggaatgcg
     9541 ctgaggccta tgaagttctc agcgatgaag aaaaacgcaa acgctatgac cagttcggtc
     9601 atgacggtct gcgcggatac ggtgtccatg actattccca tatgaatgtc agcgacatca
     9661 gcgacatctt cggggatatg ttcggggatg tatttgggga tatcttcggt ggagggcgcc
     9721 gccggagcag ttccggccgg cgtgcctcac gcgggtacga tttggaaaca gctgtggaat
     9781 taacactgga agaagtagcc cgcggaacag aaaaaactat tgaatttacg cggcaggatg
     9841 tatgccccga ttgcagcggc accggtgtta ccaaaggcaa acagcccacc cgctgtacga
     9901 tgtgcggggg cagcggtcag gtgcagaggg caggattggg gggctttttt cagatggtat
     9961 ccacctgtcc gcaatgccgg ggcgcaggcg aggtaattac cgatccctgt aaaaaatgcc
    10021 gcggcagcgg tcaggttcct aaaaaacgga aactcaatgt caagattcct gctggtgtcc
    10081 atgaggggca gggaattcgt gtcgcggggg aaggcgaacc cggtaaaatg ggcgggcctc
    10141 agggcgattt gtattgctat gtacggatta aatcgcatcc attcctaatg cgggacggtt
    10201 ccaatctggt ggttacagta cccctcagct ttacccagat ggcgctgggc agcacaattg
    10261 atgtgccgac gctggatgga acacggcagc tgaaaattcc ggccggcagc cagcatggga
    10321 gtattttgcg aattcgcgga cagggcctgc cggatatccg caccggcgtt aaaggcgacc
    10381 tgctggtgca gctttcggtg gtcattccga aaaaactgac cgctgagcag gaacggctgc
    10441 tgcgggattt tgctaaaaca gaagaacaag actcggaaac caagagtttt ttcgagaaaa
    10501 tcaggcagca ttttaataaa taattatggg cgaaaaagat cttacaaaag ctgacaaaaa
    10561 aattgctgaa ctggaagagc aaattaaaga gctgaatcag cagattgaca aactgaaggc
    10621 cgaaaagaat gaaacactag agcagcttca gcgcgtcagt gccgattatg ccaattacca
    10681 aaaacgcatt ccccggcaga ttgccgaatc ggttctttac gaaaaaaaaa cttttctgcg
    10741 ttcgctgctt cctacaatcg ataattttga gcgcgcccta tcccacggag ccgcctcaca
    10801 gagtcaacag tcgctggaga actttctcca agggattcaa ctggtgtttg agcatatgct
    10861 cgatgcgctc aagacgctgg gagtcaaacg aatcgatgca gtgggacagc catttgatcc
    10921 ggctctgcat gaagccctga tgcagcggtg cgaaccggat aagccggata atacgatttt
    10981 ggaggtatgc caaacaggtt atctgctggg cgagcaggtg ctgcggccaa gcaaggtgat
    11041 tgttaataaa tttgaggaga aggatcttcc tgccccggat gtaccgcagg aagaggcaga
    11101 aactacagac acggaatcgg aggtgtaata tgccgacata tgaatataag tgtgattact
    11161 gcaattatga gctggaagct tttcaatcta ttgctgccaa gccgctcaaa aagtgtcctg
    11221 aatgcggcaa ggcaactctt aaacggctgc tgggaacagg agcaggatta atctttaaag
    11281 gcagcggatt ttaccagacc gactatcgct ccgaaagtta taaaaaagct gccgaaagtg
    11341 aaaagtccgc tgcctcgcca gccaagaagg attctaaggc cgaatcaaaa tctgaaaata
    11401 aaagcactgc gacggaagcg tcaaaaaaat aatactgcag cagacacggg tacttcaatc
    11461 ttcaaaaacc gaattgctgt cagccgagaa agatttattt aatcagtttc gacagttctc
    11521 tgaatttttc gtgtttggaa tctccaagca gctcaaacag aagcatttcg gtcgaactga
    11581 taactgcacc tgcagacttc attcggtcca gtccgatttg tttgttttcc agtgtgcggg
    11641 ccgatgttgc atcggcgatg acatgaatat acagtccctt ttgtattaaa tctgtcgctg
    11701 tctgaaacac acatacatgt gtttcaatgc cgcacaggac ggccgtctga atgccaaggg
    11761 catccagtgc atccagaaag tttttattgc cggcacaact gaagctggtt ttatctattg
    11821 gggtaaaatc ccccagcagc gaggccagtt cattaaccgt cggaccaaga gcagatggag
    11881 cctgctgaca ccatatgata ggaatcccga gggcttttgc cgcctgtatg agaatagagc
    11941 agttcttaac aacttgttcc ggattgtgca tcacgcgcag cagcttttcc tgaatatcaa
    12001 tcagtatcag acaggtgtta tcaggtttca gcatcaaagc atccttatat ggagaaggca
    12061 gacttggttg agttactcgg catttcgctt tacggaaata tgcagcttta caggctgttc
    12121 taaaactgga gtcgtttgat tgggggcata tcgccctgtt aagatgggag gatttagctc
    12181 gggatatatg ttgtccgata actgattttc tattccggcc ggtctgcctg cataagaatc
    12241 atcccttttt aaatttgctg atgcatatcg tacggccaat cgagcaagaa gttcagggct
    12301 gtcttctctg gctaaagtgc tgatttgctc cggctgtaca ttaggaattt caatcgtctg
    12361 cttgtttgcg ctattgtaaa cattcaggat tacctgctca cagcgaggcc aaaccggctg
    12421 cagggcatca accaattcag caatcttttc agcaggggct gtaatctgat aggtcatcgt
    12481 atcatcgatg cgatttggca ccataaaacg tattaacccg atgtc
//
LOCUS       DAOTBO010000084        11875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4573_length_11875_cov_5.612014, whole genome shotgun sequence.
ACCESSION   DAOTBO010000084 DAOTBO010000000
VERSION     DAOTBO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11875
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4573_length_11875_cov_5.612014"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLE88_09595"
     CDS             <1..707
                     /locus_tag="PLE88_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76757.1"
                     /translation="GGSIVIPAAFAFIGPDGIKDIASQNLFDLGFVTMPLILQKVMFG
                     QFFGFLWFFLLFLAGVTSSISLALPAIAFLEDEFNLSRREASIVFGTVTFILCQPVIF
                     FLGNGVLGEMDFWGNSVCLVVFATIEAILFAWVFGMDKAWEELHTGSDIKIPKLYRGI
                     IKYITPLFLITILTVWLVQDWKKQFFMQDVASTNVPFVLMTRIGLLGLFVFLLLMVWI
                     AWKRRPESQNRQEESL"
     gene            704..853
                     /locus_tag="PLE88_09600"
     CDS             704..853
                     /locus_tag="PLE88_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76758.1"
                     /translation="MKWSGWIFMLISWSVIIGMFVFCMVRTLRSDAKSSKDNSSPQDS
                     KNPSC"
     gene            complement(973..1239)
                     /locus_tag="PLE88_09605"
     CDS             complement(973..1239)
                     /locus_tag="PLE88_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOM76759.1"
                     /translation="MDIRAFQQLIERRYGQRDRQRGTPGTFMWFVEEVGELATALHGN
                     DQANKEEEFADVFAWLCTLANINGIDIEKACEKYIDENRVKGYK"
     gene            complement(1255..1650)
                     /locus_tag="PLE88_09610"
     CDS             complement(1255..1650)
                     /locus_tag="PLE88_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOM76760.1"
                     /translation="MKISRSTGYALVAVGYIAQNYKDGAVLAARISKEYNIPLEYLLK
                     ILQQLVRANVLRSKRGPRGGFFLARPAEGITLLEIIEAVDGPLMSHLHLAEQTGNAPF
                     SVKMEAVCRKATEKVRDIFAESKLSEVMG"
     gene            2209..2934
                     /locus_tag="PLE88_09615"
     CDS             2209..2934
                     /locus_tag="PLE88_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76761.1"
                     /translation="METIHRHSKTTSHFSLQYLLAGSWMPERTKTIEFEKSLLDNGLD
                     FAETHLQDKAFILSRSQPSPLQIKLETPGQQVMTITVISQNPQYDLEMFCRDTQAITQ
                     AFEKTWPSEHYQLLTATGKIHHLYSSSVHAFKYLWEDRLGQLPQDFVVLGSRPVAGGG
                     LRLLIPPHSVDGSEPVSIELRIESFLRETNKLFVETIFTWPRPRIVNKMAGFGAQQQM
                     EQIENFAANEVWAFITHTRNPQA"
     gene            3280..4344
                     /locus_tag="PLE88_09620"
     CDS             3280..4344
                     /locus_tag="PLE88_09620"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOM76762.1"
                     /translation="MELTDNLNIKQFKPLTTPEQLKADLPITESAAALVADSRRQIEN
                     ILRRQDKRRMVIVGPCSLHDLEATFDFARRLQKVQKECSELLLVMRAYFEKPRTTLGW
                     KGMLYDPHLDGSYDIDFGIRQSRRILCELAAIGVPAATEFLDPIVPQYLADLISWAAI
                     GARTAESQIHRQMASGLSMPIGFKNSTDGKLSVALDAIQSAQNPHSFLGIDRNGKVIV
                     AETRGNKYGHLVMRGGSDGPNYACEYVAFAEALLRKNHILNGIVIDCSHANSHKNHKR
                     QREALLDIADQIKNGNISIAGVMLESFIKEGKQSIGAAGGLKYGVSLTDSCIGWEETE
                     ELIKVLAEAVRNSDDTPLTV"
     gene            4363..5286
                     /gene="ftsY"
                     /locus_tag="PLE88_09625"
     CDS             4363..5286
                     /gene="ftsY"
                     /locus_tag="PLE88_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOM76763.1"
                     /translation="MGLFSKTVQYLKDHLTKTRSRIASGLSSLLPFGRDLDDTLLEQL
                     EETLISDDIGVETTTRLINDLRAAYKKGAIKKTEEVLPFLKERLKSCWPPQDRQLNQA
                     ASGPTVILVAGINGSGKTTSIAKLAYMLTKNGKKVIVAACDTFRAAAVEQLTIWAERI
                     GVQIVKHKSGSDPAAVAYDACQAAIARNADYVIMDTAGRLHTQKHLMEELAKIRNVIA
                     KHIPGAPHEVILVIDATTGQNAIMQAKIFTQSIQVTGIFLAKLDGTARGGIVLAIRDM
                     LNIPVKFVGLGETPEDIAEFDPDEFVEALFN"
     gene            complement(5368..6015)
                     /locus_tag="PLE88_09630"
     CDS             complement(5368..6015)
                     /locus_tag="PLE88_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76764.1"
                     /translation="MARIAIIMVLCLAIGGCGDSNKGPTTQQEVQKSELLRQIEMKYE
                     NPEAHYQLGKLYFADGLMDKAEWEYRVAIQFNPVHYPAQAGVVKTWQVRKDAQRAKVA
                     AELYTSQAATSADASLRLGRAFQNEGLNEFALTCYQQAANLAPNSPVVFKQLGYFYLA
                     NGDKTLAEEYLRRSFELDPYQSEVAGELGRLGVIVQSPRMKVEPVQPEAPTQEVP"
     gene            complement(6136..8559)
                     /gene="gyrB"
                     /locus_tag="PLE88_09635"
     CDS             complement(6136..8559)
                     /gene="gyrB"
                     /locus_tag="PLE88_09635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOM76765.1"
                     /translation="MTEHSYDASNIKILEGLEAVRKRPDMYIGNCQEGGLHHLVYEVL
                     DNAIDEALVGRCDRILVQIHVDGSCSVEDNGSGIPTEIHEEAKKSALDVVLTTLHAGG
                     KFDKGAYKVSGGLHGVGVSVVNALSEWLEADVWRKGRHYHFECQRGVPKGPVRDIGET
                     TKRGTRITFLPDDTIFDTIEFKYEILQKRIRELAYLNGGVHITFQDDRIQKKETYHYE
                     AGIRAFVEYLNEGKVPVSEVMYFQKEDPEIGLSCELAMQYNDGYAENVLAFANNIRNI
                     DGGTHLSGFRTALTRTMNNYARSANLLKSGTAPVGEDLREGLTAVISVKVPEPHFEAQ
                     TKVRLSNPEVASFVETMVNEQLAQYLEENPAEAKRILNKAIQAAAAREAARKARELTR
                     RKGALSNTSLPGKLWDCSSRDTVNTEIFIVEGDSAGGSAKGGRDRTIQAILPLRGKIL
                     NVEKARLEKMLAHEEIGTLISALGTGIGADDFDINKCRYGKIILMTDADVDGAHIRTL
                     LLTFFFRQMPQLIEHERIYIAQPPLYEIHRKGQKDSEYILNESQMRKKTENRGLEGSR
                     LIIRNSDGSCREYEQPKLAFLVKILNEAERGIHILNRRGIVFSDFVEKYYDGSVLPIY
                     QIRSQGQDEFYYDKDSYEKRLVELNATQTEAQDLDETFFAEELHEVLRLNEICARLKE
                     DFGLDWRDYYLKQQKADSGEEQPSRFAILNGPEEFSAASLDQVPAVIRQIGSRDMEIK
                     RFKGLGEMNSEQLWETTMNPQTRTLLKVRLEDAGEADRLFSILMGDDVEKRRAFIQEH
                     ALEVQNLDI"
     gene            complement(8581..8943)
                     /locus_tag="PLE88_09640"
     CDS             complement(8581..8943)
                     /locus_tag="PLE88_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOM76766.1"
                     /translation="MACSKQVDEERLLHSVTQRIGQQRKSDIRLGEVIETFLRERSRM
                     LEQGAAVSDVWKEVIPPSLQPFCRPDKIAGHTLYIQVQPGAPMYQMQLLTSHLLEQIR
                     QQLPGSRIRTIRLVPLAV"
     gene            complement(8966..10066)
                     /gene="dnaN"
                     /locus_tag="PLE88_09645"
     CDS             complement(8966..10066)
                     /gene="dnaN"
                     /locus_tag="PLE88_09645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOM76767.1"
                     /translation="MKVKFNRAALQEALGLVTTVIPSRTPKPILQCLKITTEKESIRI
                     GATDLEAGITYLVNQVEIDKPGEVIVPAEKLSSIVRESQDDVLLMEATESAVNLVGSD
                     SRFTIYGHDPQQYPTIPVFDGTAELEVKLLGLQEGIEQTVFAAARESTRYALNGVLWE
                     VAGKKLSLVATDGRRLAKATVALEKSGKIPDGRIIVPARTMSLLLKITQHNGDETAQI
                     CFVNNQIVLASGSVVVSSNLVEGNFPKYEDIIPKDYEKKLTLNTAQMLSAVRRAALLA
                     NEDSKGIRLALDKGSLVLTSRAPETGDAQIDMAVDYSGEPIEIGFNPQFLIDVLRVIG
                     ADTFELHLGQPDRPGLIKSGSNFLYIVMPVNL"
     gene            10633..11652
                     /gene="obgE"
                     /locus_tag="PLE88_09650"
     CDS             10633..11652
                     /gene="obgE"
                     /locus_tag="PLE88_09650"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOM76768.1"
                     /translation="MFIDEAKIRVKAGDGGPGCVSFRREKFIAKGGPDGGDGGRGGDI
                     IFKADPSLDTLLDFSGRHDWVARNGSPGEGANRHGADGPDLIIPVPVGTLIYDEEYGL
                     LLKDMNEPGMQVCICRGGRGGEGNKAFATSTRQTPRFATPGKPGQERHLRLELKLIAD
                     VGLVGMPNAGKSTLISRCSDAHPRIADYPFTTLSPVLGIVELTGFRRFVMADIPGLIE
                     GAHRGAGLGHDFLRHIERTRILVHILDILPSDNSDPIENYRTIRRELELYSRVLTEKT
                     EIIVLNKADLDPERLFCEEIRKRLNKNPEDIYVISAATGEGVLQLNERLWAVVKGKTD
                     ESDSD"
     gene            11633..>11875
                     /locus_tag="PLE88_09655"
     CDS             11633..>11875
                     /locus_tag="PLE88_09655"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOM76769.1"
                     /translation="MNLIAIDIGNTNIKVAFFLDDEEKLIKSLSGYEAEIFLQLQETL
                     AECWDQIPLVKGSEQMVRDGVIVVSSVKPEWTEMVRE"
BASE COUNT         2822 a         3103 c         2897 g         3053 t
ORIGIN      
        1 tgggcggcag catcgttatt ccagcggcgt ttgcatttat cggaccggac ggcatcaaag
       61 atatcgcctc ccaaaacctc tttgaccttg gatttgttac aatgccgctg attctgcaga
      121 aagttatgtt cggccagttt ttcggctttt tgtggttttt cctgctgttt ctggccggcg
      181 ttaccagttc catctccttg gcgctgccgg cgatagcctt tctggaagat gaatttaatc
      241 tgtcgcgccg ggaagccagc atcgttttcg gcaccgttac atttatcctc tgccagccgg
      301 tcatcttctt tctcggaaac ggcgtattgg gcgaaatgga tttctgggga aattctgtct
      361 gtttggtagt atttgccaca attgaggcga tcttgttcgc ttgggttttc ggtatggata
      421 aggcgtggga agagctgcat accggttcgg atatcaaaat tccgaaactg tatcgaggca
      481 ttatcaaata tataactcct ctgtttctga ttaccatcct gacagtctgg cttgttcagg
      541 actggaaaaa acagtttttt atgcaggatg tcgccagcac caatgtgcct tttgtgctga
      601 tgacccgcat tgggctgctg gggttgtttg tgtttctgct gctgatggtc tggattgctt
      661 ggaagcgccg tcctgaatcc caaaaccgac aggaggaatc cttatgaaat ggagcggctg
      721 gatatttatg ctgatcagct ggtcggttat tatcgggatg tttgtcttct gtatggtgcg
      781 gacgctgcgt tccgatgcaa aatcatctaa agacaattcc tcgcctcaag attcgaaaaa
      841 tccttcctgc tgatgtctgt caaaaagccg tatccggctt ctcatacaat cggaaaattt
      901 gttggcttat ccgaaaaaca aaaaagcgga ttcttcaatc cgctttctgt tgcgttgttc
      961 tcaaaaacag cgctacttat agccctttac ccggttttca tcgatatatt tttcgcaggc
     1021 cttctcgata tctataccgt tgatattcgc cagtgtgcaa agccaggcaa aaacatcagc
     1081 aaactcttct tctttgttgg cttggtcatt gccgtgcagg gctgtagcca gctcgcccac
     1141 ctcttcaaca aaccacataa atgtccccgg cgtgccgcgc tggcggtccc gctgaccgta
     1201 gcgacgctca attaattgct gaaaagcgcg aatatccatt cgccgcagcc gccgtcagcc
     1261 catcacttct gaaagcttgg attctgcaaa gatatcgcgt accttttcgg tggccttgcg
     1321 gcagacagct tccattttga cgctgaatgg ggcattgccc gtctgttcgg ccaaatgcag
     1381 gtggctcatc aggggaccat ctaccgcctc aataatctcc agaagcgtaa tcccttcggc
     1441 cggacgtgcc aagaagaaac cgccgcgcgg gcctcgcttg ctgcgcagca cattggctcg
     1501 caccagctgc tggagaatct tgagcagata ttccagcgga atattgtatt ccttcgaaat
     1561 acgggcggcc aggacagcgc cgtctttgta attctgcgct atgtatccta ctgccaccaa
     1621 tgcataaccg gtcgatctgc tgattttcat agtaacctcc tgacataaag gtttatgttg
     1681 atacctttgg tccaaccaaa aaccgctaca atgactatct atactataat tatcagcaat
     1741 tgttcaggag ttgcaaggga ttatttgtcg aaaaataata atttataaaa tttcgagcgg
     1801 aataaaggat attaggggtc tttatgtgcc tttttctgtg tttcgtctga tgttgttcga
     1861 ttaattcata actactgtcg ccatctggct ttacgaacca ttagcggagg catttgctcc
     1921 ggtttgacat aggtcatatt aggtttgaca cagctccttt ggctggggta aacccgcacg
     1981 gtatattctc cttcttgggt tggatcttcc ggctccaacc atttttttag caatagttgc
     2041 agtatcttca tcgtacctat ccagacaatt tttcccttta tcgacatctc taagatgctt
     2101 cttaacatcc cgaatacttc ttttcagcga ttttccgtct ttccggaagg gctgttttcg
     2161 ttataatcaa aatgctgtat taaaagataa ccatgaaccg gagctgttat ggaaactatt
     2221 catcggcact caaaaactac ctcgcacttc agtctgcaat atctgttagc cggctcgtgg
     2281 atgccggaaa ggacaaaaac catcgaattt gaaaaatccc tccttgataa tgggctggat
     2341 tttgcggaaa cccatctgca agacaaggct ttcatcctgt cccgcagcca gccctcgccg
     2401 ctgcaaatta agctcgaaac tcccggccag caggtgatga cgattacagt tatttcgcag
     2461 aatccccagt acgacttgga gatgttctgc cgggacacac aggcgattac acaggcattt
     2521 gaaaagacct ggccgtcgga gcattaccag ctcttgaccg ccaccggcaa aatacatcat
     2581 ctttacagtt cttccgttca tgcgttcaag tatctgtggg aagaccgtct tggtcagctg
     2641 ccgcaggatt ttgtggtttt aggttcccgg cccgttgccg gcgggggact ccgcctgctg
     2701 ataccccccc attccgttga cggctccgag ccggtttcca tagagctgcg gatcgagtcc
     2761 tttttgcgcg agaccaataa gctgttcgtt gaaacgatct ttacttggcc gcggccccga
     2821 attgtaaaca aaatggccgg gttcggcgcc caacagcaga tggagcaaat cgaaaacttc
     2881 gcagctaatg aagtatgggc ctttatcacc catacccgaa acccgcaggc ctgatgcttt
     2941 ccgacggctt gagggcacag aacaaaagaa aatgccgagg gtggggagaa tgttatcttt
     3001 tttagggtaa aaatagacca aaaatggggt tctattatgc cggcggctgt gaatgccaag
     3061 cggcggaggg tctgcggaat ggtttagctg acagcagcgg agaatgttcc gtttgccgtt
     3121 tattgaagtg gtgttgggga aagttcaaga aagctttgca gcgttttgcc gcatttcaac
     3181 ctgtataaat ttgaccgtta aatggtttct tgcatttagg gggtttttcg attacatata
     3241 ttatttagac agtaccgaaa taaccggcaa ggaattggta tggaattaac agacaatctg
     3301 aatataaaac agtttaagcc gctgacaact ccggagcagc tcaaggccga tttgccgatt
     3361 accgaatctg ccgccgcact ggtggctgac tcccggcggc agatcgagaa cattctccgc
     3421 cggcaggaca agcggcgaat ggttattgtc ggaccctgca gcctgcatga cctcgaggcc
     3481 acgttcgatt ttgcaagacg cctgcaaaag gtgcagaagg aatgcagcga actgctgctg
     3541 gttatgcggg cttattttga aaaaccccga acaactttgg gctggaaagg aatgctgtat
     3601 gatccgcatc tggacggctc ttatgacatc gatttcggca tccgtcaaag ccggcgcatc
     3661 ctgtgtgagc tggccgctat tggcgtacct gctgcaaccg agtttttgga tccgattgtc
     3721 ccgcagtatc tggccgacct gatctcgtgg gccgctatcg gcgcacggac ggctgaatcc
     3781 caaattcacc ggcagatggc cagcggtctg tcaatgccca tcggctttaa aaattccacc
     3841 gacggcaagc tgtctgtcgc attagacgcc atccaatctg cccagaatcc gcactcgttt
     3901 ctcggtattg accgcaacgg caaggtcatc gttgccgaga cacggggcaa caaatacggc
     3961 catttggtca tgcgcggcgg ctcggacggt cctaattacg cctgtgaata tgtcgccttt
     4021 gccgaggctc tgcttcgcaa aaaccacatt ctcaacggca ttgtcatcga ttgcagccac
     4081 gccaattcac acaaaaacca caaacggcag cgggaagcct tgctggacat tgccgaccag
     4141 ataaaaaacg ggaatatcag catcgccggc gttatgctcg aaagcttcat caaagagggc
     4201 aagcagagca tcggtgccgc cggcggctta aaatacggcg tttcccttac agacagctgc
     4261 atcgggtggg aggaaacaga agagcttatc aaggtgctgg ctgaggccgt acgaaactcc
     4321 gatgatacac cgttgaccgt ttgataccag ccgggagaaa ccgtgggact tttttcaaaa
     4381 accgttcaat atctcaaaga ccatttaacc aaaacccgat cgcggattgc cagcggtctg
     4441 tcgtcactgc tgccgttcgg ccgtgacctt gatgacactc tgcttgagca gctggaggaa
     4501 accctcatca gcgacgatat cggcgtggaa acaacaaccc gtctgataaa tgacttgcgg
     4561 gcagcttaca aaaagggcgc aattaagaaa acggaagaag ttctaccgtt tctaaaagaa
     4621 cgtctcaaaa gctgctggcc gccgcaagac cgtcagctca atcaggccgc ttccggccca
     4681 acggtcattt tggttgcagg catcaacggc tccggcaaga ccaccagcat cgccaaatta
     4741 gcatacatgc ttactaaaaa cggcaaaaag gtcatcgttg ccgcctgcga caccttccgg
     4801 gcggcggctg tcgagcagct gaccatttgg gccgaacgca tcggcgttca gattgtcaag
     4861 cacaaatccg gctccgaccc ggctgcggtg gcctatgatg cctgtcaggc ggctatcgcc
     4921 agaaatgccg actatgtaat tatggatacg gccggccgcc tgcatacgca gaagcatctg
     4981 atggaagaac tggccaaaat ccgaaatgtc attgccaagc acatccccgg tgcgccgcac
     5041 gaagtcatcc ttgtgattga tgccaccacc ggccaaaatg ccattatgca ggccaaaatt
     5101 tttacgcaat ccattcaggt aacaggcata tttttagcca aactcgacgg cacggctcgg
     5161 gggggcattg ttctggcgat tcgcgatatg ctcaacattc ctgtcaaatt cgtggggctg
     5221 ggtgaaacac ccgaagatat cgctgaattc gacccggatg aatttgttga agctctgttt
     5281 aactaaactg tctgttattg cccttgcagg aaaagctgga caataacctt atttcattta
     5341 cggataaatg ccgtcggctg ttatgcctta cggcacttcc tgtgtggggg cttccggctg
     5401 aacaggttcg accttcatac ggggtgactg cactatcacg cccagccgtc ccagctcgcc
     5461 ggctacttcc gattgatacg gatccagctc gaagctgcgc cgcaggtatt cttccgccag
     5521 cgtcttatcg ccgttggcca gataaaaata ccccagctgc ttaaacacta ccggtgaatt
     5581 aggagccaga ttggccgcct gctggtagca ggtcagggca aactcgttaa gcccttcatt
     5641 ctgaaatgcc ctgcccagcc gcagtgacgc atccgccgaa gtggctgctt gactggtgta
     5701 caattctgcg gccaccttgg cacgctgggc atccttgcgg acctgccaag tctttaccac
     5761 acctgcctgc gctggataat gcacgggatt gaattgtatc gctacccgat attcccactc
     5821 ggctttgtcc atcaaaccgt cggcaaagta cagcttgccc agctgataat gggcttctgg
     5881 attctcatat ttcatctcta tctggcgaag cagttctgac ttctgcacct cctgctgggt
     5941 cgtagggcct ttgtttgaat ccccgcagcc tcctattgcc agacataata ccatgatgat
     6001 tgctatcctt gccatatatg cctccatcga aattttcagt ctatccgtat ccaatttttc
     6061 tctattgtct gtcaactgca ccgtttttgc aaggcaaaag ccgtatattc tttttcgtca
     6121 ctccgggctg tttttctaaa tatctaaatt ctgcacttcc agcgcatgtt cctgtataaa
     6181 tgcccgcctc ttttcaacat catcacccat taaaatgctg aacaggcggt ctgcttcgcc
     6241 ggcatcttcc agacgcacct tcagcagcgt ccgggtttgc gggttcatcg tggtctccca
     6301 tagctgttcg gaattcatct cgccaagtcc cttgaaccgc ttgatttcca tatcccgcga
     6361 gccaatctgc cgtatcaccg ccggcacctg atccagcgag gccgcactga actcctctgg
     6421 tccgttaaga atagcaaacc gtgacggctg ctcttcgccg gaatcagcct tctgctgctt
     6481 aagataataa tcgcgccagt cgagaccaaa atcctccttc agccgcgcgc agatttcgtt
     6541 taagcgcaaa acctcatgaa gctcctcggc aaaaaacgtc tcatccagat cctgtgcttc
     6601 ggtctgcgtt gcattcagct ccacaaggcg cttctcgtaa gaatctttat catagtaaaa
     6661 ctcatcctgc ccctgcgagc ggatttggta aatcggaaga acagaaccgt cgtaatactt
     6721 ctccacaaaa tcgctgaaaa caatgccgcg ccggttgaga atatgaatgc cccgttcggc
     6781 ttcgttgagt attttgacca aaaatgccaa tttaggctgc tcatactctc tgcatgagcc
     6841 gtcggaattg cggattatca gccgcgaacc ttccagtccg cggttttccg tttttttgcg
     6901 catttgggac tcattgagga tatattcgga atctttctgc cctttgcggt gtatctcgta
     6961 cagcggcggc tgggcaatat aaatccgctc atgttctatc agctgcggca tctgccggaa
     7021 gaaaaaggtc agcagcagtg tccgaatatg ggccccgtct acatccgcat cggtcattag
     7081 aatgattttg ccgtagcggc acttgttaat atcaaaatca tcagccccaa ttccggtacc
     7141 taaagcacta atcagcgtgc cgatttcctc gtgcgccagc atcttttcca gccgggcctt
     7201 ttccacattg aggattttgc ccctcagggg gagaattgcc tgaatcgtcc ggtcgcgccc
     7261 gcctttggct gaaccgcctg ctgaatcgcc ctcaacaatg aaaatctccg tgttaaccgt
     7321 atcgcggctg gagcagtccc acaatttgcc gggcagactg gtattgctca gtgctccttt
     7381 gcggcgtgtc agctcgcggg ccttgcgggc cgcttcgcgg gcggcggcgg cctgaatggc
     7441 cttgttgaga atccgcttgg cttcggccgg attttcttcc agatactgcg ccagctgctc
     7501 attgaccatt gtttcaacaa agctggcaac ttcgggatta gatagacgca ctttggtttg
     7561 tgcctcaaaa tgcggctccg gcactttgac ggatatgacc gccgtcaaac cttcccgcaa
     7621 atcctcgccg acaggggcag tccccgattt gagaagattg gcgctgcggg catagttgtt
     7681 cattgtgcgg gtcagtgccg tccggaaacc cgacaaatgc gtgccgccgt ctatattgcg
     7741 gatattgttg gcaaacgcca gcacattttc ggcatatccg tcgttgtact gcatcgccag
     7801 ctcacaggat aaacctatct cggggtcttc tttctggaaa tacatcactt cggataccgg
     7861 caccttgcct tcgttgagat actcgacaaa cgctcgaatc cccgcctcat agtggtaggt
     7921 ctctttcttc tgaatccggt catcctgaaa ggtaatatgc accccgccat tcagataggc
     7981 cagctcccga atgcgcttct gcagaatctc gtacttgaat tcgatggtat caaaaatcgt
     8041 atcgtccggc aggaatgtta tgcgggtgcc ccgcttggtc gtctcgccga tgtcgcgcac
     8101 agggcctttg ggaactcccc gctggcattc aaaatggtaa tgccggcctt tgcgccagac
     8161 gtcggcctcc agccattcgc tcagcgcatt gaccacactg accccaacgc cgtgcaggcc
     8221 gcctgaaact ttgtaagcgc ctttgtcgaa tttgccgccg gcatgcagtg tcgtcagtac
     8281 aacatccaac gcactctttt tggcttcctc gtgtatttcc gtgggaatcc cggagccgtt
     8341 gtcttctacc gagcagcttc cgtcaacgtg aatctgcaca aggatgcggt cgcagcggcc
     8401 taccaatgcc tcatcgatgg cattatccag cacttcataa acaagatgat ggagaccgcc
     8461 ctcttggcaa ttgccgatat acatatccgg acgcttgcgg acggcttcca agccttccag
     8521 aatctttata ttactggcat cataactatg ttcagtcatc ttatgtcata ctcctgaata
     8581 ctacacagcc agcggcacca gacggatggt acgtatccgg ctgccgggca attgttgtcg
     8641 gatttgctcc agcaagtggc tggtcagcag ctgcatttga tacattggcg ctcccggctg
     8701 aacctgaata tacagcgtgt gtccagcaat tttgtccggc cggcaaaacg gctgcaggga
     8761 cggcggtatt acctctttcc agacatcgga aacggcagcg ccctgctcca gcatcctgct
     8821 tcgctcccgc aggaaagttt ctatcacttc tccaaggcga atgtccgatt ttctctgctg
     8881 gccgattcgc tgcgttacac tgtgcagaag acgttcttca tccacctgtt tgctgcaggc
     8941 cataagactg cccattaggc cggtctcaca gattgaccgg catcacgata tataggaagt
     9001 tggaaccgct cttgattaga ccgggacgat ccggctggcc cagatggagt tcaaatgtat
     9061 cggcgcctat cacgcgcaga acatctatga gaaattgagg attgaagccg atttctatcg
     9121 gctcaccgct gtagtcaacc gccatatcta tctgggcgtc gccggtttcg ggagcacggc
     9181 tggtcagcac cagcgacccc ttgtccagcg ccagccgtat ccccttgctg tcttcattag
     9241 ccagcagtgc cgctcgccgg acggcactga gcatctgggc tgtattgagc gtcagttttt
     9301 tctcgtagtc cttcggaata atgtcttcgt atttcgggaa gttgccttcc actagattgg
     9361 aactcaccac gaccgagccg ctggcaagga caatctgatt gtttacaaaa cagatttggg
     9421 cggtctcgtc tccgttatgc tgggtgatct tcagcagcag cgacatagtg cgggccggca
     9481 caatgatgcg gccgtccgga atcttgccgc tcttttccag agccacagtt gctttggcca
     9541 aacgccggcc gtcggtagcg actaacgaca gctttttgcc cgcaacctcc caaagaacac
     9601 cgtttagggc gtagcgtgtg ctttcgcgtg cagccgcaaa gaccgtctgc tcaatcccct
     9661 cctgaagacc gagcagtttt acttccagct cggcagtacc gtcaaagacg gggatagtcg
     9721 ggtattgctg cgggtcatgt ccgtagatgg taaaacggct gtctgaaccg accaagttaa
     9781 cagccgattc cgtcgcttcc attaacaaaa catcatcttg gctttcacga acaatcgatg
     9841 acaacttttc agccggcaca atcacttctc cgggcttatc gatttcaacc tgattgacca
     9901 gatatgtaat accggcttca agatcggtag caccgattcg aatggattct ttttccgttg
     9961 tgattttcag acattgcagt atcggcttgg gtgtcctgct gggtattacc gtcgttacca
    10021 aacccaatgc ttcctgcaaa gctgcgcggt tgaacttgac tttcatagct tcctcattta
    10081 ttggtttttt gtctgtcctt gccaagtctg aatcgacccc ataatgtacc cgaaaactgt
    10141 gttcttgaaa agagtttgaa acggtaaaaa attaagattt tgaacaattc ccgggccaag
    10201 ggcatagatg cagccggaac tgaggatctt aagaagtaat agatggttta atctttataa
    10261 tcgtatttgt cataagttct gtaaaattgt aaactatata agcacgcaat ttgcaagatt
    10321 tgtataataa agtacttata attaagatgg taaaatgctg attgcggaga agctgttaga
    10381 aaagctgtta gcagctatga tgaaacgaaa aaaataatgt gattaaatgt aataatatgg
    10441 aaaatagaac agaataatta acaacacaga cacaagattt aacaggttat caacagaaga
    10501 aaaatttatt tacctcttta tagacaaaaa aagtattgta tcggtgtata ataaacatat
    10561 tgttatcggt caacaggacg tttataatct cgcacttaaa gaaataaata tcaatacatt
    10621 aaatatccac aattgtttat tgatgaagca aaaattaggg taaaggcagg cgacggcggt
    10681 cccggctgcg tcagttttcg cagggagaag tttattgcca aaggcggccc tgacgggggt
    10741 gacggcgggc gcggcggaga tattatattt aaggccgatc cttcgctgga tacgttactg
    10801 gatttttccg gcaggcatga ctgggtagca cggaatggct ctcccggcga aggtgcgaac
    10861 cggcatggag ccgatggacc ggatttgatt attccagttc ctgttggaac gctgatttac
    10921 gacgaagaat acggcctgct tctgaaggat atgaatgagc cgggaatgca ggtctgcatc
    10981 tgccggggcg gccgcggcgg cgagggaaac aaggcctttg caacgtccac ccgccaaacg
    11041 ccgcgttttg caacacccgg caaacccggt caagaaaggc atttgcggct tgaattgaag
    11101 cttatagccg atgttgggct tgtcggtatg cccaacgccg gcaaaagcac gctgatttcg
    11161 cgctgttccg atgcacaccc cagaattgct gattatccct tcacaacgct gtcgccggtt
    11221 ctggggattg tggaactgac cggatttcgc agatttgtaa tggctgacat acccggcctg
    11281 atagagggag cacaccgagg ggccggtctg ggacacgatt ttctccgcca tatcgagcga
    11341 acgcggattc tggtgcatat cctcgatatt ctgccgtctg acaactcaga ccctatagaa
    11401 aactaccgta ccatccggcg ggagctggag ctgtatagtc gggttcttac agaaaagact
    11461 gaaatcatcg tacttaataa agctgatctt gacccggaaa gacttttttg cgaagagata
    11521 cgcaagcgcc tgaataaaaa tcccgaagat atatatgtga tttcggccgc cactggcgaa
    11581 ggcgtccttc agcttaacga gcggctctgg gctgttgtaa aaggaaaaac agatgaatct
    11641 gatagcgatt gatataggaa ataccaacat caaagtggct ttttttctcg acgacgaaga
    11701 aaagctcatc aaaagcctca gtggttacga ggccgaaatc ttcctgcagc ttcaggaaac
    11761 actggccgaa tgctgggacc agattccgct ggtcaagggc tccgaacaga tggtacggga
    11821 cggcgtcata gttgtcagct ccgtaaagcc cgaatggaca gaaatggttc gcgag
//
LOCUS       DAOTBO010000085        11795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4611_length_11795_cov_5.586457, whole genome shotgun sequence.
ACCESSION   DAOTBO010000085 DAOTBO010000000
VERSION     DAOTBO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_11795_cov_5.586457"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLE88_09660"
     CDS             complement(<1..790)
                     /locus_tag="PLE88_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOM76770.1"
                     /translation="MDEIGWSIILLCLLTPASLFFTLNSMALRSFSRVKLQEAFRQLG
                     REQWIDELIASVEPLAIACGSIRLLCNAGIIFLIVSLFADHHRLSAFAAAFVILEIFT
                     LIIPHAWAKHAGEYILPRTYRLLQLFTLLLKPLLAIVELHDRFVQRLAGVPKADSEQA
                     QEERQEEILSVVEQGRMEGIVDEEEVEMIENVLELGGTTAEEIMTPRTDLIAIEIHDD
                     LPKILETIQDKGHSRIPVYEGTIDNIVGLIYAKDLLTEIGKTPGQ"
     gene            complement(802..1284)
                     /gene="ybeY"
                     /locus_tag="PLE88_09665"
     CDS             complement(802..1284)
                     /gene="ybeY"
                     /locus_tag="PLE88_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOM76771.1"
                     /translation="MKKNPNKRLKINFHPDVPAADIDLSRLRALIVNICRQFKISQAV
                     ITVRIVNDAGIMDIHQQFLGQTATTDVISFDLSDEFEPGQTFELAVNAQMAARQAARR
                     GHSAEAELALYITHGLLHNLGFDDRTPSEARKMHAMEDSILQQNGFGVVYSDDKQSNA
                     "
     gene            complement(1334..2878)
                     /locus_tag="PLE88_09670"
     CDS             complement(1334..2878)
                     /locus_tag="PLE88_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOM76772.1"
                     /translation="MTPLLKKKVSSRRQQVRNNLAMERLARLASAVNGPYPKAIVILI
                     LFIGAVTAALGVEINQETIYLYGLTQFKPWLEIISLLIIVAAVSIGMSLYLIHYRPNI
                     LHKPARLIAIACLFWLLLAVTRAFSLKEEWKPMAVGAAVCGAIILTIAYDQRFAIGMM
                     MFYIMLAVFAAEQTASVDLLLTMAAGALSCCFSLREIRTRKKLIEVSLLATIAVFLMR
                     TALGIIQNQPVKEYILQAGIAAAAAFVVGIFIQAFLPIIEQTFGIATSMTLMDYSDAN
                     QPLLRKLAMDAPGTFSHSLLIGSIAEAAAESIGANGLLCRVGAYYHDIGKIHKPAYFV
                     ENQMGSSSRHEQLSPAMSQLVIAGHVKDGIEIAKEFGLPSVLREFIETHHGTTLMEYF
                     YHEAKKRQDERQSPVWESEFRYPGPKPRSKEAAIVMMADAAESAARSLTDHTLAKVET
                     LVHTIAMKRLQDGQFDECDLTLRELSLIEASLSKSLTAHHHGRIAYPKTEEKPKANGT
                     NGTAAQ"
     gene            complement(2875..3828)
                     /locus_tag="PLE88_09675"
     CDS             complement(2875..3828)
                     /locus_tag="PLE88_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOM76773.1"
                     /translation="MDLKIQIEPDAQRLELFGPADSHLRQLCQTLGVKITARNGTVLV
                     SGDASAVHTTADLIGRMQKRLIQRGQLNSDDVDELLNRCRQTIDRDNPDVIEVYSRKG
                     IIEPFTKGQMVYIQTMYKNDLTFCIGPAGTGKTYLAVAVAVSMLKRKQIRKIVLARPA
                     VEAGERLGFLPGDLQAKVNPYLRPLFDSLEDMMDFGQMQKFMEMDIIEVIPLAFMRGR
                     TLNEAAVICDEAQNTSRSQMLMFLTRLGRGSKMIITGDITQTDLAPGEKSGMVEAIET
                     LRHIPGIGFVTLDKCDIVRHKLVHQIVQAYDEKKQKENNLK"
     gene            complement(3831..4682)
                     /locus_tag="PLE88_09680"
     CDS             complement(3831..4682)
                     /locus_tag="PLE88_09680"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOM76774.1"
                     /translation="MLKYRLIFGTVMTVFFVGLILFDGWLDGSLLEAVPDKPVQAPFF
                     TLLIALLTIPAQFEFGNLIKNTGAHLFKSVTIPATILLASGCLCFQFIYSFYVLAFSF
                     LALFIVQALKFGTEGTIRNVGASLFSIAYLGLLCWFILRVRILWGPVVLLFFIFTVKA
                     SDIGAYTIGRLFGKHKLCPRISPGKTWEGLGGAVLFGTIVSIGISACCGIMSVMSAAI
                     FGAVFGILGQMGDLAESMLKRDAAVKDSSASVPGFGGLLDVIDSPLATAPAALLTFSL
                     LLASTGS"
     gene            complement(4685..5473)
                     /locus_tag="PLE88_09685"
     CDS             complement(4685..5473)
                     /locus_tag="PLE88_09685"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOM76775.1"
                     /translation="MKDHQTVEEKRKQTAQRLGIHPDAVPRHIAIIMDGNGRWALERG
                     LPRYRGHEKGAETVKVIAQHCVDSGIEYLTLYSFSMQNWKRPREEVDFLMYLYAAYLE
                     GIRPSLMENNVRLAHLGRREPLPPTVLKALDDTIAATSANTGMILGLALNYGSQAEIV
                     DAVRSLARKVKDGSIEPDSINEQDISNHLYTAGWKDPDLVVRTSGELRISNFLLWQVS
                     YAEFYVTSAYWPDFTPEEMNKAILAYAGRQRRFGDIKPNFSSQG"
     gene            complement(5470..6756)
                     /locus_tag="PLE88_09690"
     CDS             complement(5470..6756)
                     /locus_tag="PLE88_09690"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOM76776.1"
                     /translation="MNCCVVGLQWGDEGKGKVVDILAQNADIVVRYGGGANAGHTVII
                     GDTKFALHLMPSGAVREKTACVIANGVVVDPGVLIDEINGLKAQHIHMEGRLFISENA
                     HIVLDYHKLEDQLREAAMGKNKIGTTARGIGPCYADKVGRVYALRMADLCRPDTVLKN
                     KLQTIIDHKNKLFGALYGAEPLAFDAIYEKCLHYSSVLKPFICNTTRYLHEALDAGRN
                     ILFEGAQGALLDLDHGTFPFVTSSNASALGLGPGSGVPASRVDRFIGVVKAYATRVGA
                     GPFPTEQDNEIGSRIREKGHEYGTTTGRPRRCGWFDAVSVRYTVKLGGVSEIAMMHLD
                     TLAGMREIKLCRAYKIGGKETTFFPASVEELAEAECIYQTLDGWNEDLSTIREYEALP
                     QPVKDYIKAVESIVGVPVTMVGVGPKRSQAIFRNPV"
     gene            complement(6861..7316)
                     /locus_tag="PLE88_09695"
     CDS             complement(6861..7316)
                     /locus_tag="PLE88_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76777.1"
                     /translation="MAVEAPLSRYKKNNLLIMAVLLIGLGIWFYYDGYHNAAFIAKHT
                     QQDGSPDSTLNFNRKAPPFIIGGGIVLAIYLAAIRGRKIIADDTALRCGRLSIAYDAI
                     EQINKTHFSSKGFFVITYNDQGQRRQLKLSDRMYDNLPAVLDYIAAKIQ"
     gene            complement(7339..8091)
                     /locus_tag="PLE88_09700"
     CDS             complement(7339..8091)
                     /locus_tag="PLE88_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76778.1"
                     /translation="MANSFGSFCDGLFLELCVTTQLELPSSRETILAFFERLQRLHPE
                     MNNFSRRDGGEYVLEQESQGQRIQWVGLEADRITAGCADPDSLEQAYALHAQVLELIP
                     YMLGVSPLDIDSLDITFGLDFDYQGNHDEVIAAALLNTSSFGTLFEMPQVRAISCCPS
                     VIIALSDDCRLQARVAIESRTTAYDVRSEKYKSDEPISLYFSVRRYPLSSPPFDTLAS
                     FREQCRIAENLMFDKIIPHFVQPLNNAIAQRR"
     gene            complement(8243..10762)
                     /locus_tag="PLE88_09705"
     CDS             complement(8243..10762)
                     /locus_tag="PLE88_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76779.1"
                     /translation="MACDYSIQVCRQLEADFQKCSLYRPMRIAHYDAGTVLCYDLNPI
                     EPGPVIQVRLCVERFVGGGFAGQVYKVHLDDICADGKSVDRAAALVRGKSYALKILIP
                     PSAGSRFFRNFLYAVGFQGPFQLQVNPAAARAGALWQKLIRAAAQIRFNDPLCVNDIH
                     GTFVDSELGSCGEISDWIDGRTWRLEVDDHLDQLNRWKKGKSVDLTVLGSPEYRSKYM
                     FMTEFVKLLHEIGAYEFARQYEWTTCKSQPNVLKRIDTNAEPSKGLTAVDFRAGLTLL
                     AVGPMSPGDFKLIWQGIKRGSLVQFDRGNIKKLEDYISQHPDLKNVLPNAEKLLCELK
                     QCESIYRDSIPDITHNHFRLLYDRRLWGQILDSAVTGWKTQNLIDAEDIFRNSPLKTI
                     VFLLLGLIPFIGKLCRRLWAKADWRKHYKSLLTDCSYFYRSVKGRMAEMLIQWHRSGR
                     LGEDNAIAVFNSPVLFLLHLPLSVLPAGLHRLITDPKVLQNKLHFVFVRPIQLYFNAS
                     LREQWLRDMVRQGQKKHILSDEDASTILSQLNEPFIQKYLISLVVHLMTLPVTQIVSV
                     AVAALFYWTHPNMPDAERAAAVAGILVLFQVIPISPGSFCRGLYTTVIAIRDRNFKDY
                     NIALFLSYFKYVGYLAFPIQMTYRYPALARFMAAHWATDAVHIVPVFGERGALLEHWI
                     FCLFYNWPLTIRRRMRCISEIRRSLPVRLWHIIPCVLLAVYVLSAAHYGYCKTGRVPE
                     KDNLWFIKPLFCLIVFIPLAVGWAVSRFAGGLARGKRILSAASAGLLTGIGYSIAAWA
                     LEQNWNIETEVLVPLIWRGFALSIFSVIGAILAEISQKETQ"
     gene            complement(10859..11344)
                     /locus_tag="PLE88_09710"
     CDS             complement(10859..11344)
                     /locus_tag="PLE88_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOM76780.1"
                     /translation="MTKEELLTTVFGLGKLPAAPGTFGSLASVLLYLLLGYVYPSANW
                     YVMALLAALGTWVCIEYGPQMTAITGKKDPPEVVADEAAGQALVMLAITLLNPAKICN
                     AGVTGFVLFRLFDIFKPFPCKQLEKLKGGYGILADDLMAAVYAAAVFIVLRRFLPAFF
                     G"
     gene            11590..>11795
                     /locus_tag="PLE88_09715"
     CDS             11590..>11795
                     /locus_tag="PLE88_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76781.1"
                     /translation="MNPRSVWFFILFVYILSLSLSVQGQEELSISVSTPTIPLETEEA
                     SARQLRIYRETLFQGSTEDVRVDAA"
BASE COUNT         3040 a         3062 c         2822 g         2871 t
ORIGIN      
        1 actggccggg cgttttgccg atttcggtca gcaaatcctt tgcataaatt agtccgacaa
       61 tattgtctat cgtcccctca taaaccggta tccgcgaatg ccccttgtct tgaatggtct
      121 ccagaatctt gggcaggtca tcgtgtattt caatggcaat caggtcagta cggggagtca
      181 taatctcttc agccgtcgtg ccgcccagtt caagcacatt ttcaatcatc tccacttctt
      241 cttcgtccac aatgccctcc attctgcctt gttcgacgac actgaggatt tcttcctgcc
      301 tttcttcctg cgcttgctcc gaatcggctt tgggaacacc ggccagacgc tggacaaacc
      361 gatcgtgcag ctcaactatt gccagcaggg gcttcagcag cagcgtaaac agctgcagca
      421 gccgatatgt tcgaggcagg atgtattcgc cggcatgctt ggcccacgca tggggaatta
      481 tcagcgtgaa aatttccaag atcacaaatg ccgccgcaaa ggccgacaaa cggtgatggt
      541 cggcaaataa ggatacgata aggaaaataa tcccggcatt gcataacagc cggatagagc
      601 cgcaggcaat tgccagcggt tcgacgctgg caatcagctc atcaatccat tgctctcgcc
      661 ccagctgtcg aaaggcctcc tgcagcttta cacgggaaaa cgaccgcagc gccatgctgt
      721 taagagtaaa aaacagcgat gccggcgtca ggaggcaaag caggataatt gaccatccga
      781 tttcatccat gaatttgtct cctatgcatt tgactgctta tcgtcagaat agactacacc
      841 aaaaccattc tgttgtaaaa tcgaatcttc catggcgtgc atcttgcgtg cttcggaagg
      901 ggttctgtca tcgaagccga gattgtggag aagaccatgg gtgatataca aggccagttc
      961 tgcttcggca gaatgccctc ttcgggcggc ctgacgtgcg gccatttgtg cattcaccgc
     1021 cagctcaaat gtctgtcccg gctcaaactc gtccgaaagg tcgaaactga tgacatccgt
     1081 tgtggcagtt tgccctaaaa actgctggtg gatatccatt attccagcat cattgacaat
     1141 ccgaacagtg atgaccgcct gtgaaatctt aaactgcctg cagatattga caatcagcgc
     1201 ccgcagacgg gacaggtcaa tatcggcagc cggcacatcg ggatggaagt tgatttttag
     1261 acgtttattg ggattttttt tcattgtttc tgattttttt taaggaaatt acgctgactg
     1321 tgcctgctta tttctattga gctgctgtac cgttagtccc attggctttg ggtttttcct
     1381 ctgttttcgg gtaagcaatg cgtccatgat gatgcgctgt aagcgatttg gatagactgg
     1441 cctctatcag actgagttcc cgcagggtca ggtcgcattc gtcaaattga ccgtcctgaa
     1501 gccgcttcat cgcaatggta tggaccagcg tttcgacttt ggcaagcgta tggtcagtca
     1561 gggagcgggc agcgctttcg gcggcatcgg ccatcattac aatggcagct tcttttgagc
     1621 ggggcttggg gccgggatag cggaattcgg attcccaaac cggactttgc cgctcatcct
     1681 gtcgtttttt ggcttcatgg tagaaatatt ccatcagggt agtgccgtga tgggtttcta
     1741 tgaattcccg cagaacggag ggcaatccga attctttggc aatttcaatg ccgtctttga
     1801 catgacctgc gataaccagc tggctcatgg ccggggacag ctgctcatgc cggctgcttg
     1861 aacccatctg gttttccaca aaatacgccg gcttgtgaat tttcccaatg tcgtggtaat
     1921 aggctcccac gcggcaaagc agtccgttgg cgccgatgga ttcggcagct gcttctgcaa
     1981 tcgagccgat cagcagactg tgactgaaag tccccggagc atccattgcc agttttcgca
     2041 gcaggggctg attggcgtcg ctgtaatcca tcagggtcat gcttgtagcg atgccaaatg
     2101 tctgttcaat gatgggcagg aatgcctgaa taaagatgcc gaccacaaaa gctgccgccg
     2161 cagcgatgcc ggcctgtaga atgtattcct tgaccggctg gttttggatg atgcccagtg
     2221 ctgtgcgcat cagaaagacc gcaatggtag ccagcagcga tacttcaatc agttttttgc
     2281 gggtacggat ttcccgcaga ctgaagcagc agctcagggc gccggccgcc atggtcagca
     2341 gcagatcgac cgaagctgtt tgttcggctg caaagaccgc cagcataata taaaacatca
     2401 tcattcctat ggcgaagcgc tggtcatagg caatagttaa aatgatagcc ccgcacacgg
     2461 cggcacctac cgccataggt ttccattcct ctttgagcga aaaagcacga gtgacggcca
     2521 gcagcagcca gaacaggcag gcaattgcaa tcagacgggc cggtttgtgc agaatgttgg
     2581 gccggtagtg gatcagatac aaagacattc caatgctgac ggcggccaca ataatcagaa
     2641 gcgaaataat ttccagccac ggcttgaatt gggtcaatcc ataaaggtaa attgtctcct
     2701 gatttatttc cacacccaat gcggctgtga cggctccgat gaacaggatt agaatgacga
     2761 tcgctttcgg ataagggccg ttgacagcgg acgcaaggcg agccaaacgc tccattgcca
     2821 gattattgcg cacctgctgg cggcgggaac tgactttctt tttcagaagc ggcgtcattt
     2881 cagattgttc tccttctgct ttttctcgtc ataggcctga acaatctgat gaaccagttt
     2941 atgccggaca atatcgcatt tgtccagtgt tacaaatccg attccgggaa tatgccggag
     3001 cgtctcgatg gcttctacca tgccgctttt ttcccccggc gccaaatctg tctgggtaat
     3061 atcacctgta ataatcattt tagagccgcg tcccagccgc gtcagaaaca tcagcatctg
     3121 ggagcgggat gtattctgcg cttcgtcaca gataaccgcc gcttcattca gcgtacggcc
     3181 tctcataaaa gccagaggaa taacttcgat aatatccatt tccatgaact tctgcatctg
     3241 cccgaagtcc atcatatctt ccaagctgtc aaacaagggg cgcaaataag gattgacctt
     3301 ggcctgcagg tcgccgggca aaaatcccag gcgttcgccc gcttccacag ccggccgggc
     3361 cagaacgatt ttccggattt gcttgcgttt gagcatcgaa actgccacag caaccgccag
     3421 ataggtcttg cctgtcccgg cagggcctat acagaaggtc aaatcatttt tatacatcgt
     3481 ttgtatatag accatctgcc ccttggtaaa cggctctatg attcctttgc gcgaatacac
     3541 ttcaatgaca tcgggattat cgcggtcgat ggtctggcgg catcggttga gcaattcatc
     3601 cacatcgtca ctgtttaatt ggcctctctg gataagccgt ttctgcatcc ggccaatcaa
     3661 atctgcggtg gtgtgaactg cggatgcatc ccctgaaacc agtacggttc cgtttctggc
     3721 agtaatttta actcccagcg tctggcacaa ttggcgcagg tgggaatctg ctggaccaaa
     3781 taattccagc cgttgtgcgt ctggttcaat ctgtatcttt aaatccaaaa ttaacttcct
     3841 gtgctcgcaa gcaaaagact gaatgttaaa agtgccgccg gtgcagtcgc caaaggcgaa
     3901 tcaatgacat ccagcaggcc tccaaatccc ggcacgctgg ctgaggaatc cttgacggcg
     3961 gcatcccgct tcagcatcga ttcggccaaa tcgcccatct ggcccagaat tccaaatacg
     4021 gccccgaaaa ttgccgctga cataacagac attataccac aacacgcaga aataccaata
     4081 gagacaatgg tcccaaacag aactgctccg cccaagccct cccatgtttt tccggggctg
     4141 atgcggggac agagtttatg cttgccaaac agccggccaa tcgtgtaagc ccctatatca
     4201 gaagctttta cggtaaaaat aaagaacaat aaaacaaccg gcccccataa aatgcgaact
     4261 cttaaaataa accagcacag aagtcctaaa taagcaatcg aaaaaagaga tgccccgaca
     4321 tttcgaatcg tgccttcagt gccgaatttc agcgcttgta cgataaacag tgccagaaat
     4381 gaaaatgcca gcacatagaa agaatatata aactgaaagc acaaacagcc ggaagccagc
     4441 agaattgttg ccggtattgt aacgctttta aataggtgtg cgccggtgtt ttttatcaaa
     4501 ttgccaaact cgaattgtgc cggtattgtc aagagggcaa ttaacagcgt aaagaagggc
     4561 gcttgaaccg gcttgtctgg tacggcttct agcagcgaac cgtccagcca gccgtcaaac
     4621 aggatcaggc ccacgaaaaa taccgtcatc actgttccaa agataagtcg atatttaagc
     4681 atagtcagcc ctgtgacgag aaattcggct tgatgtcgcc gaagcgccgc tgccggccgg
     4741 cataagccaa gatggcctta ttcatctctt caggtgtaaa atccggccag taggcgctgg
     4801 ttacatagaa ctctgcatac gagacctgcc acagcagaaa attgctgatg cgaagctcgc
     4861 cgcttgtacg cactaccaaa tccgggtctt tccatccggc ggtgtaaaga tgattgctga
     4921 tatcctgttc attgatggag tccggctcaa tggaaccgtc tttgaccttg cgggccaagg
     4981 accggacggc atcgacaatc tctgcctgtg agccgtagtt cagtgccaag cccagaatca
     5041 taccggtatt ggcgctggtg gcagcaatgg tgtcatccaa tgctttcaat actgttggcg
     5101 gaaggggctc ccgccggccc agatgcgcca gacgcacatt gttttccatc aaactgggcc
     5161 gtatcccctc aagataagcc gcatacaaat acatcaaaaa gtccacttcc tcgcgaggac
     5221 gcttccagtt ctgcatgctg aaggaataca gcgtcagata ttcaatgccg ctatctacgc
     5281 aatgctgggc aatgactttt acagtctcgg ctcctttctc gtgtccgcga taccgcggca
     5341 gtccccgttc tagtgcccac cgcccgttgc cgtccatgat gatggcgata tggcggggta
     5401 cagcatccgg atggatgccc agccgctgtg ctgtctgctt gcgtttctct tcaaccgttt
     5461 gatgatcctt cataccggat ttctgaagat ggcctgactt cgtttgggcc ccacacccac
     5521 catcgtaacg gggacaccga caatggactc aaccgctttt atataatctt tgaccggctg
     5581 aggcagcgct tcatactcac gaatagttga caagtcctca ttccagccgt ccagcgtttg
     5641 gtagatgcat tcagcttcgg ccagttcctc gacgctggcg gggaaaaaag tggtttcctt
     5701 tccgccgatt ttgtaggctc tgcacagctt gatttccctc attccggcca aagtatccag
     5761 atgcatcatt gcgatctcgc tgacgccgcc cagcttgacg gtatagcgta cggagactgc
     5821 atcaaaccat ccgcagcgcc gcgggcgtcc ggtggtcgtg ccgtattcat gccctttttc
     5881 acggatgcgg ctgccgattt cgttgtcctg ctccgttggg aaaggccctg ccccgacgcg
     5941 tgtagcatag gccttgacca caccgataaa ccggtctacc cgtgaagccg gaacgccgct
     6001 tcccggcccc agccccagag cgctggcatt ggaactggta acaaagggaa atgttccgtg
     6061 gtccaaatcc agcagcgctc cctgtgcgcc ttcaaaaaga atattccggc cggcgtccag
     6121 tgcctcgtgc agatagcgtg tggtgttgca gataaacggt ttgagaacgc tgctgtaatg
     6181 aagacatttt tcataaatgg catcaaacgc cagcggttca gccccgtaaa gtgctccaaa
     6241 cagcttgttt ttatggtcga taatagtctg cagtttgttt ttcaataccg tatcgggccg
     6301 gcacaggtca gccatccgca gcgcataaac gcgtcccact ttgtcggcat agcagggacc
     6361 aatgccccgg gcggttgtac cgattttatt tttgcccata gccgcttcgc gaagctggtc
     6421 ttcaagcttg tgatagtcca gaacaatgtg ggcattttcg ctgataaaaa gccgtccttc
     6481 catatgaatg tgctgggctt tgagcccgtt gatttcatcg ataagcaccc ccgggtcaac
     6541 aacaactccg ttggcaataa cacaggcggt tttctcccgc acggcaccgg acggcatcag
     6601 atggagggca aatttggtat ccccgataat aacagtatgc cccgcattgg ccccgccgcc
     6661 atagcgaacg acgatatctg cattttgggc cagaatatcg acgactttgc ctttgccttc
     6721 atcgccccac tgcaacccca ccacacagca attcatagta ctcctttata aacaagctcg
     6781 gcctcgatcc gatttttaag atgaacaagc attggtcagc aaaacccgca acaaagcgtc
     6841 tccttgccga ataccggagc ttattggatt ttggctgcaa tataatccaa taccgcaggc
     6901 aaattgtcgt acatgcggtc gctgagtttt aactgacgac gctgaccttg gtcgttgtag
     6961 gttatcacaa aaaaaccttt ggaagaaaaa tgggttttat tgatttgttc tattgcatca
     7021 taagcgatgc ttagacggcc gcagcgaagc gcggtgtcat cggcaatgat ttttctgcct
     7081 cgaatggctg ccagataaat ggccagcaca atgcctccgc caattataaa cggcggggct
     7141 ttgcggttga aattcagcgt gctgtccggc gagccgtcct gctgggtatg cttggcgata
     7201 aaagcagcat tgtggtatcc gtcataataa aaccagatgc ccagtcctat cagcagcaca
     7261 gccataatca gcagattgtt ctttttgtag cggcttaaag gtgcttctac agccattgtg
     7321 ctttttgcct ccatagaatc agcgtcgttg agcaattgca ttattaagcg gctgaacaaa
     7381 atgcggaatg attttgtcaa acatcaggtt ttcagcaatc cggcactgtt cccgaaacga
     7441 cgccagtgtg tcaaacggag gcgacgaaag cggataccgg cgcacggaaa agtagaggct
     7501 gatgggttca tcggatttat acttctcgct gcgcacatcg taggcggtcg ttcgggattc
     7561 aatcgccaca cgggcctgca gccggcagtc gtccgacagg gcaatgataa cagatggaca
     7621 gcagcttatg gcccggacct ggggcatttc aaacagcgtc ccgaagctgg aggtattcag
     7681 cagcgctgca gcgataacct catcatggtt gccctgataa tcaaaatcca gtccgaatgt
     7741 aatgtccagt gaatcgatat ccagcggact gacacccagc atatagggaa tcagctccag
     7801 cacttgggcg tgcagggcat aggcctgttc gagcgaatcc ggatctgcac agccggcggt
     7861 gatacggtct gcttccagcc caacccactg gatacgctgc ccttgagact cctgctccag
     7921 aacatattcg ccgccgtcgc gacgcgaaaa attgttcatt tctgggtgca gtctctgaag
     7981 ccgctcaaaa aacgcaagaa tggtctcccg gctggaagga agttccagct gggttgtcac
     8041 acataattcc aaaaacaggc cgtcacaaaa cgacccgaaa ctgtttgcca taaaaaagtc
     8101 tcctaaaaaa cctaataact ctattatggc agaaaaatta gtcaattaca agaaggaaaa
     8161 cttcttataa agcaggtgaa aatcaacttg ccggagatgg ctatccataa tatctctatt
     8221 agaaagccca gccggaaaga gattattggg tttctttctg gctgatttca gccaaaatcg
     8281 ccccgatgac gctgaaaatc gaaagtgcaa atccccgcca aatcagcgga acaagcacct
     8341 ctgtttcgat attccaattc tgttccagtg cccatgcagc gatagaatag ccaatgccgg
     8401 ttagaaggcc tgccgatgct gccgacagaa tacgcttgcc ccgtgccaat ccgccggcaa
     8461 aacggcttac agcccatccg actgctaacg gaataaaaac aatcaggcag aaaagaggct
     8521 taataaacca gagattgtct ttttcaggta ccctgccggt tttgcagtat ccatagtgag
     8581 cagcactcaa cacataaaca gccaaaagta cacagggaat gatatgccac aaccgaaccg
     8641 gcaacgagcg gcgtatttcg ctgatgcagc gcatccggcg gcgtatggtc agcggccagt
     8701 tgtagaaaag acaaaagatc cagtgttcca gcaaggcccc gcgctctcca aaaaccggga
     8761 caatatgaac cgcgtcggta gcccaatggg ctgccataaa tcgggccagc gcaggatagc
     8821 gataagtcat ttgtatgggg aaggccaagt atcctacata cttaaaatag cttaaaaaaa
     8881 gagcgatatt gtagtctttg aaattcctgt cccgtattgc aataaccgtt gtataaagac
     8941 cgcggcagaa tgaaccgggt gaaatgggaa tcacttggaa caggaccaaa attcccgcaa
     9001 cagcggctgc tctttcagca tcgggcatat tggggtgagt ccagtaaaac agggcagcta
     9061 ccgcaaccga gacaatctgg gtaaccggca gcgtcattaa atggactact aaactgatca
     9121 gatatttttg tataaaaggt tcgttaagtt gtgagagaat cgttgaagca tcctcatcgc
     9181 ttaaaatatg cttcttctgc ccctgccgaa ccatatcccg cagccattgt tcacgcagag
     9241 aggcattgaa atacagctga atcggccgca caaatacaaa atgcagtttg ttttgaagta
     9301 cttttggatc ggttattaac cgatggagac cggccggcag gaccgacagc ggcaggtgca
     9361 gtagaaaaag aacaggggag ttgaaaacag caatcgcatt gtcttcaccc aatcgcccgc
     9421 ttctatgcca ctggattagc atttcagcca tgcggccttt aacagaccga taaaaatagc
     9481 tgcagtcggt caacaaactc ttataatgct ttcgccagtc tgcctttgcc caaagccggc
     9541 ggcatagttt gccaataaaa ggaatcaggc cgagcaggag aaagacaatg gtttttagag
     9601 gagagtttct gaaaatatct tcagcatcaa taagattttg cgttttccac cccgtaaccg
     9661 cgctgtctaa tatctgtccc caaagccgcc tgtcatacag caggcgaaaa tgattatgcg
     9721 tgatgtccgg aattgaatcc cgataaattg actcgcattg ctttaattca caaagcaatt
     9781 tttctgcatt cggcagaaca tttttcaggt cgggatgctg ggaaatataa tcctcgagtt
     9841 tttttatatt tcccctgtca aattgaacca gcgagccgcg tttgattccc tgccaaatca
     9901 gcttgaaatc ccccggactc attggcccaa cggcaagcag cgtcaatccc gcccgaaaat
     9961 caacggcggt caagcccttg ctgggctcgg catttgtatc gattcgtttc aggacattgg
    10021 gctggctttt acaggtcgtc cattcgtact gccgggcaaa ctcgtaagcg ccaatctcgt
    10081 gcagcagttt aacaaactcc gtcataaaca tatatttaga gcggtattca ggagagccca
    10141 gaacagttaa atcgacggat ttgccttttt tccatcgatt cagctggtcc agatggtcgt
    10201 ctacttccag ccgccatgtc cggccgtcaa tccaatcgct gatttcgccg cagctgccta
    10261 attctgaatc aacaaaggtg ccgtggatgt cattgacgca aagggggtca ttaaatcgaa
    10321 tctgggcagc agcacggatc aatttctgcc atagtgcccc ggctcgggct gccgcgggat
    10381 tgacctgcag ttgaaatggg ccttgaaaac cgacagcata taaaaaattc ctgaaaaaac
    10441 gcgaccctgc agaaggagga attaatattt ttaacgcata actttttccc cgcaccagtg
    10501 cagcggcacg atccacagat tttccatctg cacaaatgtc atccagatgg actttataaa
    10561 cctgaccggc aaaaccgccc ccgacgaatc tttcaacaca caagcgcact tgaataaccg
    10621 gtcccggttc aattggattg aggtcatagc ataaaaccgt cccagcatcg taatgtgcga
    10681 tccgcatcgg acggtataag ctgcactttt gaaagtcggc ctcaagctga cggcaaacct
    10741 gaatcgagta atcgcaagcc attgagtgct ccatattaaa acaaaggcgg cctaaaccgc
    10801 ctgtaaaatt gtctgcatgg gctttttatc gacacataac ttattttgct tcaatagttt
    10861 atccgaaaaa ggcaggcaga aaacggcgta aaacgataaa cacggccgct gcatatactg
    10921 cggccatcag gtcgtcggcc aaaatcccat accccccttt aagtttttct aactgtttgc
    10981 aaggaaaggg cttgaaaata tcaaacagcc gaaacagaac gaatcctgta acacctgcgt
    11041 tacagatttt tgccggattc aatagagtaa ttgccagcat cactaatgct tgcccggccg
    11101 cctcatctgc tacgacttcc ggcgggtctt tttttcctgt tattgctgtc atttgtggcc
    11161 catactctat gcatacccat gttcccaatg cagccagtaa tgccataaca taccaattgg
    11221 cggaaggata cacgtatccc aaaaggaggt ataacaatac cgacgccagt gaaccaaacg
    11281 ttcccggcgc tgcaggcagc ttgccaagcc caaaaactgt tgtcagcaat tcttcttttg
    11341 tcatctgctt ccaacgaaca taatatgctg aaatatactt ttagaaagaa tagttttttt
    11401 atgttttttc atcgaaaagg ggaacttttc gcacaatctg gagtattttt tattatagca
    11461 gacaaaaaaa caacaaaaaa gcggttcgtg tttgacggat ttgggttttt tgatactata
    11521 agcacaaaat tgccgaaata gtaaccattg aattgtcctg caggtatgaa agtgcgttat
    11581 ggagtgcccg tgaaccccag gagtgtatgg ttttttatcc tgttcgttta tattctgtcc
    11641 ctgtcgttat ctgtacaggg tcaggaagag ctgtctatta gtgtatccac cccaaccatt
    11701 ccactagaga cagaagaggc gtccgctcga caattgcgga tttaccgcga aactctgttt
    11761 cagggcagca cagaagatgt ccgtgtggat gctgc
//
LOCUS       DAOTBO010000086        11566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4748_length_11566_cov_4.947094, whole genome shotgun sequence.
ACCESSION   DAOTBO010000086 DAOTBO010000000
VERSION     DAOTBO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4748_length_11566_cov_4.947094"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..864
                     /locus_tag="PLE88_09720"
     CDS             115..864
                     /locus_tag="PLE88_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76782.1"
                     /translation="MKTTVWSIQAVIGIACLCGCSTPPHTYKQVFDRYYATTLKSSTS
                     AEVLASIADPQTDLLSQSDSVVAAWGKQGRKDRTHWFNMVAFDEQQMNAVRKYGFILE
                     ETRWGWNRVPKPALRFDAELVMEPDVLNAAYASSNEKQIAMIKKTQEHFSKDRLEVVF
                     DGQTLNSSMMMVQQALHSVLTKLSQSPAYAAKLAQPEGLDFDHPTLGESRVRLLMEGD
                     IVKLKIKAGKPWFQRYYIDDPFEEHPDVKYM"
     gene            913..>1440
                     /locus_tag="PLE88_09725"
     CDS             913..>1440
                     /locus_tag="PLE88_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOM76783.1"
                     /translation="MFTKVTIIGDGGMGSVCAMLLCENGVQTTLWGYDAAQLARIAAA
                     KENILFLPGCPLPDSLIYQADDALAAADAQLLVSAVPCQFTRGIWQRLKPHIRPGIPI
                     VSVTKGVENGTLLRPTQIIAEVLGPSHPLAALSGPTIADEVARRLPATATAACSDAAL
                     AGRIQQTFSTPMFRIY"
     assembly_gap    1441..1450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1451..1916
                     /locus_tag="PLE88_09730"
     CDS             <1451..1916
                     /locus_tag="PLE88_09730"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOM76784.1"
                     /translation="NDDLTGVELAGAMKNVIAIAAGIIDGIGAGDNAKAALITRGLAE
                     IQRLGTALGAKAKTFAGLSGLGDLVTTCISPKGRNRSFGERIGRGLSVKEALGATHSV
                     VEGASTCQSIVQLAAKHSIEMPITQAVHEVIQGRKTVREAIAELMTRQLKAE"
     gene            complement(1993..3306)
                     /gene="hflX"
                     /locus_tag="PLE88_09735"
     CDS             complement(1993..3306)
                     /gene="hflX"
                     /locus_tag="PLE88_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOM76785.1"
                     /translation="MREQIKVHQERAILVGAFLKTDLKKQANQDVLEELTALAESAGA
                     IVVGRFIQRMPQIHAATYIGSGKAAQLADKVKQAGADVVIFDNDLSPGQIRELEKIVN
                     VKVLDRSELILDIFATRAQTRQAKLQVELAQLEYTYPRLTRMWSHLDTVTGAAGGTGA
                     GAVGGIGTRGTGEKQLEIDRRLVSKRITELKRDIAAIDKRKMREIEGRRDVFKISIVG
                     YTNAGKSTLMNALTGAGVYVEDKLFATLDTRTRRWQPAAGVEVLVSDTVGFVSKLPHH
                     LVASFKATLEETVHADLLLHVVDASSPQAFEQIQSVQAVLKELDCHRKDTLVLLNKAD
                     RIANRSVFEAVQTVFPDALTISAKTGMNLDVLAGRIVQKVSGGQVRIRITADAADGRL
                     QSFLRMAAADLLVEYRDGSVCIEATLGKNQLAHLQRLNACQIEYL"
     gene            3494..4849
                     /locus_tag="PLE88_09740"
     CDS             3494..4849
                     /locus_tag="PLE88_09740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM76786.1"
                     /translation="MATETINYYNERRLRQKTLRLVVYEGIFSMIAIGFQQTFYIPFL
                     NAMGASRLEVGIGAGLPALMTGLIQLWTPQLLRREKGYKKLVLISVFGHAVSFLPFTL
                     IALSRSPNAVWLAIAAGAVNAAMMGLGNSAWSDWMSYLVPRRRRGAYFSMRNRILTLL
                     QLLTSLAAGQFLDTFPGRTLLIFSLIWTLAFLMRSIGGGYMAAQYEPPAVRRRAPEQG
                     AFIDFLRRLHSEAFGRFVLAFALLNLGANFSGPFFAVYMLNDLQLNYLAYTVLVSIPS
                     LTIVLMMSFWGRLCDRIGYVMPMRFFSTVVMGLPLVWIVTRNYWLLAAVQVLAGISWG
                     GMQMASFNYSLDAVGSHNRLRSISYLNVITGICICAGCTLGGLLEPHLPPVSGNQIYS
                     VFLVSVLIRIIPTLIFQTLPEDKPRHAKMTALERFFFDPRLSLRSGLDRTIFGRGKQT
                     L"
     gene            4969..8211
                     /locus_tag="PLE88_09745"
     CDS             4969..8211
                     /locus_tag="PLE88_09745"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOM76787.1"
                     /translation="MRRIIWTAAAITAVAGCVTTQTFLSANHRSDLQAVNLRCEHRTE
                     PLGIDVVNPRLSWTLQSRQRGQRQTAYRVLVASDAALLKADIGNLWDSGRVESSQTFG
                     LEYAGKPLESGSSCFWKVCVWDKDGKPSAWSKPALWSMGLLKKEDWQAEWVGLDLPAS
                     ALELPAVLKKAVWIWSEAGAAGSTAAGQRFFRRSFELPADWNGGDVVCYITADNSFRL
                     FVNGQLVRTDKNFNVIHEVPIGSYLRGGRNTLAVLAINEGNAPNPAGLLAAVQIPAGS
                     GAAVEIVSDAQWRCSDKAAAGWTGPDCDDSDWTPAAVLGPLGTAPWSKTTLARLTLPP
                     ARYLRCEYAVADKPVRQARLYATALGIYQLYLNGQRVGDDYFSPGWTDYSRRIYYRTY
                     DVTALLQRGTNALGAVLADGWFAGYVGYARQRNHYGSKLRLLGQLVIDYADGTRQVVA
                     TGPGWKGSLGPIQQADFLQGETYDARLEEPGWNAPGFDDSRWQKADVGGNEVSPLVQA
                     AVSEPVAVFARVKPVSVSEPAAGVYVFDMGQNFAGVVQITVKGKAGQKIVIRHAERLN
                     PDGTIYTANLRSAAATDTYICKGGRTEIWQPYFTFHGFQYVELTGLDSKPALDAVVGL
                     ALSSNTPAAGTIECSDAVVNKIQSNAVWTQRANFIDIPTDCPQRDERLGWTGDAQVYI
                     NTACYHNDVQAFFTKWLTDLTDAQRADGQFPMVAPLKVAGDDGGPAWADAGVICPWTI
                     YKMYGDKRIIETHYEAMKKFIAFCKNRCTSDLLPPAQFHCFGDWLNIGDDTPRDVIYT
                     AYFGCSTKLLAQMAEALDKPQEAAEYQNLFEQIKASFCRAYVQPDGRIKGDSQTAYVL
                     AIAYELLDAQMQAKAAAHLVRRIEECGWHLSTGFVGTKDLMLVLAKIGRNDVAYRLLH
                     NDTFPSWGFSVKHGATSIWERWNGWTPEQGFGDPEMNSFAHYSFGAVCQWMFENIGGI
                     QTDGPAFGHILLKPQPGGTLQWAKTAYNSIRGPIRTAWEIRDGRFICEVSIPPNTTAT
                     LYLPAAGHQAVYESNKPISEAEGILAVSHQPGAVIAELESGDYRFSSYLP"
     gene            complement(8244..9080)
                     /locus_tag="PLE88_09750"
     CDS             complement(8244..9080)
                     /locus_tag="PLE88_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM76788.1"
                     /translation="MTKKPIQPQDGILVTLNLTKRFEDQLVFDSISLAIEEGKTTVII
                     GPSGCGKTVFMKHLIVLERPTEGQVYFKGQRIDHLDERSLAKIRMHFGFCFQMGALFD
                     SLTVYENITFPVRQHYKTANPRMLDELVKQKLALVGMDGFQHQYPASLSGGQRKRVAL
                     ARAIALNPEVILYDEPTTGLDPITSDVINELIIKLQKELKITSVVVTHDMKSAYKIAD
                     RIVMLYNGRIIADGDADHIRNHPHDVVQQFIHGRVGEAELAALRLGGTRFETQFTPDD
                     FK"
     gene            complement(9103..9915)
                     /locus_tag="PLE88_09755"
     CDS             complement(9103..9915)
                     /locus_tag="PLE88_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM76789.1"
                     /translation="MIIESLGEWTITKVGNTGRFFRFIWASMAALVHAVLRPRDYPLI
                     LTQMYAIGVQSVPVVMTTGAFVGMILAIQAYGQLASMGFEERLGVLINIAVVKELGPV
                     LAAVMLAGRVGGALTAELGTMNVTEQIAAVESMGTNPIHYLVAPRVLACLLLTPFLIV
                     YADLLGILGGWFISVYQLKINAGAYWNYSAIGVERWDICTGILKGFFFGGAIALISCY
                     KGFTCRQGAHGVGQACTEAFVGCFMTILFLNYVFAVVFDAVYKCFWPLRMLL"
     gene            10125..10847
                     /locus_tag="PLE88_09760"
     CDS             10125..10847
                     /locus_tag="PLE88_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76790.1"
                     /translation="MPAKRLQHAFTLIELLAVLAVIALMLSVVLPALRQAKEAARTVQ
                     CKTLMKNYALAFYSYFWQTNTLFPLSIQDWQNNIPMHPWHSLDEFRALVDLEPTGPEY
                     RIRRTGQLQEYKPAFPRRFICPSARYALEHPEDGLYPMNRSFGLNAHVYYFKDYVRRR
                     LESQSSRISCMADALDWWFSYWQCDIYLQTGEQWLGFDTYGSAAFRHRDKANISYWDG
                     HVEQMAAGELKEHLNQWRYYNP"
     gene            10940..11294
                     /gene="ssrA"
                     /locus_tag="PLE88_09765"
     tmRNA           10940..11294
                     /gene="ssrA"
                     /locus_tag="PLE88_09765"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            11373..>11566
                     /locus_tag="PLE88_09770"
     CDS             11373..>11566
                     /locus_tag="PLE88_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76791.1"
                     /translation="MRCLTESAESRRTLKGVFVIKIFLIDAAPYGIYFIIKHQIWPKG
                     DWGAGIGRGLSGAIVGIGRY"
BASE COUNT         2374 a         3243 c         3490 g         2449 t
ORIGIN      
        1 caaggccaag gaagtttaat ccgataagac acaagggcgg ccgctgcaag ccgccccttt
       61 ttggctttaa tcttgtgcag ccgatagagt gaaaaccagt taaggagtgt aacggtgaaa
      121 acgacggtat ggagcataca agcggtaatc gggattgcct gtctgtgcgg ctgcagcacg
      181 ccgccgcaca cctacaagca ggtgtttgac cggtattatg cgacgacgct caagagcagc
      241 acctcggccg aggtactggc gtcgattgca gatccccaga ccgatttgct cagccaaagc
      301 gacagcgtgg tggcggcctg gggcaagcag ggccgtaaag accgcaccca ctggttcaat
      361 atggttgcct ttgatgagca gcagatgaat gccgtccgca agtacggttt tattctggaa
      421 gaaacccgct ggggatggaa tcgggtgccc aagccggctc tgcgcttcga tgcggagctg
      481 gtgatggagc cggatgtgct caatgccgct tatgccagca gcaacgaaaa gcagatagcg
      541 atgattaaga agacccaaga gcacttctcc aaagaccggc tggaagttgt cttcgacggc
      601 cagacgctga acagctcgat gatgatggtc cagcaggcgc tccacagcgt gctgaccaag
      661 ctgagccagt cgccggcgta tgccgccaag ctggcccagc cggaaggatt ggactttgac
      721 catccgacgc tgggcgaaag ccgggtgcgt ctgctgatgg aaggcgacat tgtgaaactc
      781 aaaatcaaag ccggcaagcc gtggtttcag cggtactata ttgacgatcc ttttgaggaa
      841 cacccggacg tcaaatatat gtagtgccct gccggcggac aagccggacg attcagcggg
      901 cccaatgcct gtatgttcac aaaggtaacc atcatcggcg acggcggaat gggcagcgtc
      961 tgtgcgatgc tgctgtgcga aaacggcgtc cagaccaccc tgtggggcta cgatgcggcg
     1021 cagctagccc gcattgcggc ggccaaggaa aacatcctct ttctaccggg ctgcccgctg
     1081 ccggattcgc tgatctatca agccgatgat gcgctggctg ccgccgatgc gcagcttctg
     1141 gtgtcggcgg ttccgtgcca gtttacgcgg ggcatctggc agcggctcaa gccccatatc
     1201 cgcccgggca ttccgattgt cagcgttacc aaaggagtcg aaaacggcac cctgctgcgg
     1261 ccgacgcaga ttattgccga ggtgctgggg ccatcgcatc cgttggcggc cctgtcgggg
     1321 ccgactatcg ccgatgaagt agcccgcagg ctgccagcca ccgccacggc tgcctgttcg
     1381 gatgcagcgc tggcaggacg cattcagcag accttcagca cgccgatgtt ccggatttac
     1441 nnnnnnnnnn caacgatgac ctgacagggg tggaactggc cggagcgatg aaaaatgtca
     1501 ttgccattgc cgccggcatc attgacggca tcggcgccgg cgacaacgcc aaagccgccc
     1561 tgattacccg gggtctggct gaaatccagc ggctgggcac cgcactgggg gccaaagcca
     1621 aaaccttcgc cggcctgagc ggcttgggcg atctggttac cacctgcatc tcgcccaaag
     1681 gacgcaaccg cagcttcggc gaacgcatcg gcaggggctt gagcgtcaag gaggcactgg
     1741 gcgcaacgca cagtgtggtc gagggggctt ccacctgcca gtccatcgtt cagctggctg
     1801 ccaagcacag tatcgaaatg cccatcacgc aggccgtaca cgaggttatc caaggccgca
     1861 aaaccgtccg ggaagccatc gccgaactga tgacccgcca gctgaaggcg gaatgattct
     1921 ccatcgtacg cgggcttgtg gccagcgctt caacggcgcc agcacgatgc ctgcgcggca
     1981 ttgaagtgca agttacagat attcgatttg gcaggcgttg aggcgctgga ggtgggctaa
     2041 ctggtttttg cccagcgtgg cttcgatgca gactgagccg tcgcggtatt cgaccagcag
     2101 gtcggctgcc gccattcgga gaaaactttg cagccggccg tccgcggcat cggctgtaat
     2161 gcgaatgcga acctgcccgc cggagacctt ctggacgatc cgcccggcca gcacatccag
     2221 attcatgccg gttttggccg aaatggtcag ggcatcggga aaaacggtct gaacggcctc
     2281 gaataccgag cggttggcga tgcggtcggc tttgttgagc agcaccagcg tgtccttgcg
     2341 gtggcaatcc agctccttga gcacggcctg cacgctttga atctgctcaa acgcctgcgg
     2401 gctggaggca tcgacgacat gcagcagcaa atcggcatgg acggtttctt cgagggttgc
     2461 cttgaacgag gcaaccagat ggtgcggcag tttgctgaca aatccgacgg tatcgctgac
     2521 cagaacttcc acgccggcag caggctgcca gcggcgggtt cgggtatcca gcgtggcaaa
     2581 gagtttatcc tccacataga ctccggcacc ggtcagggcg ttcatcaggg tgcttttgcc
     2641 ggcattggtg tagccgacaa tggagatttt gaacacatcg cggcggcctt caatctcgcg
     2701 catcttgcgt ttgtcgatag cggcgatgtc gcgcttgagt tcggtaatcc gcttgctgac
     2761 cagccggcgg tcgatttcca gctgcttttc gccggtgccg cgggtgccga tgccgccgac
     2821 agcgccggcg cctgttccgc cggcagcgcc ggtaaccgta tccagatggc tccacatgcg
     2881 ggtcagacgc ggataggtgt attccaactg tgccagctct acctgcagct tggcctgacg
     2941 ggtctgggca cgcgtggcga agatgtccaa aatcaattcg ctgcggtcca gcactttgac
     3001 attgacgatt ttttccagct cccgaatctg tccgggggac aggtcgttgt cgaatatcac
     3061 cacatcggcg ccggcctgtt tgactttatc ggccagctgg gcggccttgc ccgagccgat
     3121 ataggttgcc gcgtgaattt gcggcatccg ctggataaac cgccctacca caatcgctcc
     3181 ggcactttcg gccagtgctg tcaattcttc cagcacatcc tgattggctt gttttttaag
     3241 gtcagtcttc aggaaggcgc caaccagtat tgcccgttcc tgatgaacct ttatctgctc
     3301 acgcattttt tccaattgcc agcatcgcct ccgcagtttg cagcagtttc tgttaaatta
     3361 ggcaaatttg cttttcttga caaattatac cataccggcg acactttcac aagaccgccg
     3421 gggacaatgg agaaatttga cttgctgccg gcaatcggct gattacaatg acggactttc
     3481 accagaacgg aaaatggcaa cagaaactat caattattat aacgaacggc ggctgcgtca
     3541 gaagacgctg cggctggtgg tgtatgaagg catattctcg atgatagcca tcggctttca
     3601 acagaccttc tacatcccgt ttctgaacgc gatgggcgcc agccggcttg aggtgggcat
     3661 cggggcaggg ctgccggccc tgatgaccgg gcttattcaa ttgtggacac cgcagctgct
     3721 gcgacgggaa aagggctata agaaactggt gctgatcagc gttttcggac atgcggtcag
     3781 ttttctgccg tttacgctga ttgcgctgtc ccgtagcccc aacgccgtct ggctggcaat
     3841 agccgccggg gctgtcaatg cggcgatgat ggggcttggc aactcggctt ggagcgactg
     3901 gatgagctat ttggttccgc gtcggcggcg cggggcgtat ttctccatgc gcaaccgcat
     3961 cctgacgctg ctgcagcttc tgacatcgct ggcggccgga cagtttctgg atacttttcc
     4021 cggccggacg ctgctgattt tctcgctgat atggacactg gcttttctga tgcgcagcat
     4081 cggcggcggg tacatggccg cccagtatga accgccggcc gtgcgccgcc gagcgccgga
     4141 gcagggagca ttcattgact ttctccggcg tctgcacagc gaggccttcg ggcggtttgt
     4201 gctggccttt gcgctgctga acctcggggc caatttcagc ggtccgtttt ttgccgtcta
     4261 tatgctcaac gacctgcagc tcaattatct ggcctatacg gtgctggtca gtatcccgtc
     4321 gctgacgatt gtgctgatga tgagcttctg ggggcggctg tgcgaccgca tcgggtatgt
     4381 gatgccgatg cggtttttca gcacggtggt gatggggctg ccgctggtct ggattgtcac
     4441 gcgcaattac tggctgctgg cggcggtaca ggtgctggcg ggcatttcat ggggcgggat
     4501 gcagatggcc agcttcaact actcactgga tgccgtcggc tcgcacaacc gcctgcggtc
     4561 gatatcgtat ctgaacgtca ttaccggcat ctgcatctgt gccggctgca cgctgggcgg
     4621 ccttcttgag ccgcatctgc cgccggtcag cggcaaccag atttactcgg tctttctggt
     4681 cagcgtgctg atacggatta tcccgacgct gattttccag acgctgcccg aagacaagcc
     4741 gcgccatgcc aaaatgacgg cgctggagcg gttcttcttt gacccgcgcc tgtcgctgcg
     4801 cagcggactg gaccgcacca tcttcggacg gggcaaacag acactttgac cggcagcaga
     4861 atggatttgc ccgatgcgcc ggtcttctat ataatggagc tttcaaacag gcacagccca
     4921 gccgctactg tgccgccgaa caaggaacct tttggaggta gagcaacaat gcgtcgtata
     4981 atatggacag ccgccgcaat cacagcagta gccggatgcg tgacaacaca gacatttctg
     5041 tctgccaatc accggtcaga cctgcaagcc gtcaatcttc gctgcgagca tcgtaccgag
     5101 ccgctgggaa tcgatgtcgt caatccgcgc ttgagctgga ccctgcagtc ccgtcagcgc
     5161 ggccagcggc agacggccta tcgggttctg gtcgcctcgg atgcggcgct gctgaaggct
     5221 gacatcggca acctgtggga ttcaggccgt gtcgaaagca gtcaaacctt tgggctggag
     5281 tatgccggca agccgctgga atcgggcagc agctgcttct ggaaggtgtg cgtctgggac
     5341 aaagacggca agccttcggc gtggagcaag ccggcgctgt ggtcaatggg gctgctgaaa
     5401 aaagaggatt ggcaggcgga gtgggtgggg ctggacctgc cggcgtcggc actggagctg
     5461 ccggcagtgc tcaagaaagc cgtctggata tggtcagagg ccggcgccgc tggaagcaca
     5521 gcggccggcc agcggttttt caggcggtcg tttgagctgc cggccgactg gaacggcggt
     5581 gatgttgtct gctatattac agccgacaat tcgttccggc tgtttgtcaa cgggcaactg
     5641 gttcggaccg acaagaactt taatgtcatc cacgaagttc ccatcggcag ttatctgcgc
     5701 ggcggacgga atacattggc cgtgctggca atcaacgagg gcaatgcgcc caatccggca
     5761 ggtctgctgg cggcggtgca gattccggca ggcagcggag cggctgtcga aatcgtttcc
     5821 gatgcccaat ggcgctgctc ggacaaggca gcagccggct ggacaggacc ggactgtgat
     5881 gattcagact ggacccctgc ggcggttctc ggaccgctgg gcacggcgcc gtggagcaaa
     5941 accacactgg cacggttgac cctgccgccg gcgcggtatc tgcggtgcga gtatgcggtg
     6001 gccgacaagc cggtccggca ggcacggctg tatgccacag cgctggggat ttatcagctt
     6061 tacctcaacg gccagcgggt tggagacgat tatttttcgc ccggctggac cgattacagc
     6121 cggcggattt actaccggac atatgatgta accgctctgc tgcagcgggg caccaacgcc
     6181 cttggcgctg tgctggctga cgggtggttt gccggctatg tcggctatgc ccgccagcgg
     6241 aatcactacg gcagcaagct gcgtctgctg gggcagctgg tgattgacta cgccgacggg
     6301 acgcggcagg ttgtcgccac aggccccggc tggaagggct cgctggggcc gattcagcag
     6361 gctgatttcc tgcagggtga gacctacgat gcccgtctgg aggagccggg ctggaacgcg
     6421 ccgggctttg acgacagccg ctggcagaaa gccgatgtcg gcggcaatga ggtcagtccg
     6481 ctggtgcagg cggctgtttc tgaaccggtg gcagtatttg cccgggtaaa gcccgtctcg
     6541 gtcagcgagc cggctgcggg ggtgtatgtg tttgatatgg ggcagaactt tgccggggtt
     6601 gtgcagatta cggtgaaagg aaaggccggc cagaagattg tgattcgcca cgccgaacgg
     6661 ctcaatcccg acggcacaat ttacaccgcc aacctccgca gcgctgcggc aaccgatact
     6721 tacatctgca aagggggccg gacagaaatc tggcagccgt actttacctt ccacggcttc
     6781 cagtatgtcg agctgaccgg tctggacagc aaaccggctc tggatgccgt tgtcgggctg
     6841 gcgctgtcca gcaatacgcc ggcagccggc acaatcgaat gctccgatgc ggtggtcaat
     6901 aaaatccagt ccaacgctgt ctggacacag cgggctaatt tcatcgatat tcccaccgac
     6961 tgcccgcagc gggacgagcg gctcggctgg acgggcgatg cgcaggtgta catcaacacg
     7021 gcctgctatc acaacgatgt gcaggcgttc tttaccaagt ggctgactga cctgacggat
     7081 gctcagcggg ccgacggcca atttccgatg gtggcgccgc tgaaggtggc cggcgatgac
     7141 ggcggaccgg cgtgggcgga cgccggcgtt atctgtccgt ggacgattta caagatgtac
     7201 ggcgacaagc ggatcattga aacgcattat gaggcgatga agaagtttat cgccttctgc
     7261 aaaaaccgct gcacgtccga tctgctgccg ccggcacagt tccactgctt cggcgactgg
     7321 ctcaacatcg gcgatgatac gccccgggat gtgatttata cggcgtattt cggctgcagc
     7381 accaagctgc tggcccagat ggccgaggcg ctggataagc cgcaggaggc tgccgaatac
     7441 cagaatctgt ttgagcagat caaggcctca ttctgcaggg cttatgtcca gccggacggc
     7501 cgcatcaagg gcgattcgca gacggcctat gtgctggcga ttgcctatga gctgctggat
     7561 gcccagatgc aggccaaagc cgcagcgcat ttggtccggc ggatagaaga atgcggctgg
     7621 catttatcga ccggatttgt cggcaccaaa gacctgatgc tggtcttggc taaaatcggc
     7681 cgcaacgatg ttgcctaccg tctgctgcac aacgatacat tcccttcgtg gggcttttcc
     7741 gtcaagcacg gcgccacgag catctgggag cggtggaacg gctggacgcc cgagcagggc
     7801 tttggcgatc cagagatgaa ctcgtttgcg cattattcgt ttggggcagt ctgccagtgg
     7861 atgtttgaaa atatcggcgg cattcagaca gacgggccgg cctttgggca tattctgcta
     7921 aaaccccagc cgggcggcac gctccaatgg gccaagacgg cgtacaactc cattcgcggt
     7981 ccgatacgga cagcttggga aatccgagac ggccggttca tctgcgaggt gtcgattccg
     8041 cccaatacta cggcaacgct gtatctgccg gcggccggtc atcaagccgt gtatgaatcg
     8101 aacaagccca tcagcgaggc cgagggcatc ttggctgtat cgcatcagcc gggcgctgtc
     8161 attgcggaac ttgaaagcgg cgattaccgg ttcagctcgt atctgccctg accggcggca
     8221 aaagccccgc acagtctttg ggcttatttg aagtcgtcgg gggtaaactg ggtttcgaag
     8281 cgggttccgc ccagccgcag ggcggccagt tccgcttcgc cgacgcggcc gtggatgaac
     8341 tgctggacca catcgtgcgg atgattgcgt atgtggtcgg cgtcgccgtc ggcgatgatg
     8401 cggccgttgt agagcatcac aatgcggtcg gcgattttgt aggcgctttt catatcgtgg
     8461 gtaaccacca cgctggtgat tttcagctcc ttctggagct tgataatcag ctcgttgatg
     8521 acatcggagg tgatagggtc cagtccggta gtcggttcgt cgtagagaat gacttcggga
     8581 ttgagcgcaa tggcccgcgc cagagccacg cgcttccgct ggccgccgga aagcgatgcc
     8641 ggatactggt gctgaaagcc gtccattccg accagcgcca gtttctgctt gaccagttca
     8701 tccagcatac ggggattggc tgtcttgtaa tgctggcgga cgggaaatgt aatattttca
     8761 tagaccgtca gactgtcgaa cagcgccccc atctgaaagc agaagccgaa atgcatgcgg
     8821 attttggcca gcgaccgctc gtccagatgg tcgatgcgct gccccttgaa gtagacctgt
     8881 ccttcggtcg gccgctccag cacaatcaga tgcttcataa agaccgtctt gccgcagccg
     8941 gaaggcccga tgataacggt tgttttgcct tcctcaatgg ccagcgagat gctgtcaaac
     9001 accagctggt cctcaaaccg tttggtcagg ttgagcgtta ccagaatgcc gtcttggggc
     9061 tgtatcggtt tttttgtcat agcgctgtgt ccgcctgagt ctctacagca gcatccgcag
     9121 cggccagaag catttgtaaa cggcatcgaa aaccactgca aatacataat tcaagaacaa
     9181 aatcgtcata aagcagccga caaaggcctc tgtgcaggcc tgacctactc catgagcgcc
     9241 ctgccggcag gtaaagccct tgtagcagct tatcagggcg atcgccccgc cgaagaaaaa
     9301 tcctttcagg atgccagtgc agatatccca gcgctccacc ccgatggcgc tgtaattcca
     9361 gtaggcgccg gcgttgattt tcagctgata gacgctgata aaccagccgc ccagaatgcc
     9421 cagcaggtcg gcatagacaa tcagaaacgg cgtcagcagc aggcaagcca gcacccgcgg
     9481 ggcaaccaga taatggatcg ggtttgtccc catactttcg actgcggcga tttgttcggt
     9541 tacattcatc gtccccagct ctgccgtcaa tgccccgccg acccgtccgg ccagcatgac
     9601 agccgcaagc accggtccca actccttgac gacagcgata ttgatcagca cgcccagccg
     9661 ttcttcaaaa cccatactgg caagctggcc ataggcctga atggccagaa tcatccccac
     9721 aaacgcccct gtcgtcatca ccacaggaac cgactgcacc ccgatggcat acatttgggt
     9781 caatatcagc ggataatcgc gcggacgcag taccgcatgc accagcgcag ccattgaagc
     9841 ccagataaat ctgaaaaagc ggcctgtatt gccgactttt gtgattgtcc actcacccag
     9901 cgattcaata atcattatac atccgtatca agacacatta taaccagtat ttagactgtc
     9961 tgcgtccatt tatccatcct gaattatcgg tctttttcca tcctgaactt aagccggaat
    10021 tgggccgctc aacgtgcaaa ttcggcaggc ggcaaaactt tttcaaccga cttttctggc
    10081 gttgagtata taatagagaa tagagccgta tcggacaaac aacaatgcct gcaaaaagat
    10141 tacagcatgc gtttacgctg attgagctgc tggcggtgct ggcggtaatc gcgctgatgc
    10201 tgagtgtggt tctgccggct ctgcgtcagg ccaaagaggc cgcccgcacc gtgcagtgca
    10261 aaacgctgat gaaaaactac gcattggcct tttacagcta tttctggcag accaatacgc
    10321 tgtttccgct ttcgattcag gactggcaga acaatatccc gatgcatccg tggcattctc
    10381 tcgatgagtt tcgggcgctg gtggatttgg agccgacggg gccggaatac cgcatccgcc
    10441 gaacgggaca gttgcaggaa tacaagccgg cctttccgcg gcggtttatc tgtccatcgg
    10501 cacggtatgc gctggagcat ccggaagacg gtctgtatcc gatgaaccgc agcttcggcc
    10561 taaatgcgca tgtttattac ttcaaagatt atgtgcggcg ccgtctggaa agccaaagca
    10621 gccgtatttc ctgcatggcc gatgcgctgg actggtggtt cagctactgg cagtgcgata
    10681 tctacctcca gaccggcgag cagtggcttg gatttgacac ctacggctcg gcggcctttc
    10741 gccaccggga caaggccaat atttcgtact gggacggcca tgtggagcag atggctgccg
    10801 gcgaattgaa agagcatctc aaccagtggc gctattataa cccttagggc ctctgggatt
    10861 ttgctgggcg gcaggggcag gggcatctct tgcagcaggc gtcggaatcg ggtataatgg
    10921 aagcgttctt tgaaaaatcg ggggcgactg gcttcgaccg gacaggtaga gggccaggcg
    10981 gcatgcccag gatgacggtt ggcctggtaa atcatccgtt actaaattta actggcaact
    11041 accagtatgc aatggctgct taattaagca gcccgtcctg cacgacccct tcccgcgggt
    11101 tttgcaaggg cgccgaacag cgggatagcc gttctgtttt tttgcggcgg gacggcgaaa
    11161 ctgaagcaaa atagcgaggc attcggcctg tcgttcggca ggatgccaag cgaaacttaa
    11221 acgaacgact atgcatgtag aggcctgtcc tgaaatgtct cgggacgggg gttcgaatcc
    11281 ccccgcctcc agttttgcgc tgctttcaag ggttggggtt cttgctccaa agaaggcggt
    11341 attttgcttt catacgccct ttcggctgcg ttgtgcgctg tttgacagaa tccgccgaat
    11401 cccgccgaac actcaagggg gtttttgtca tcaagatttt cttaatagac gctgcccctt
    11461 atgggatcta tttcattata aaacatcaaa tctggccaaa gggtgattgg ggggctggaa
    11521 tcgggcgggg gttaagcggc gcaatcgttg ggattggccg gtactg
//
LOCUS       DAOTBO010000087        11518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4775_length_11518_cov_5.433482, whole genome shotgun sequence.
ACCESSION   DAOTBO010000087 DAOTBO010000000
VERSION     DAOTBO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_11518_cov_5.433482"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLE88_09775"
     CDS             <1..441
                     /locus_tag="PLE88_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOM76792.1"
                     /translation="PGIIALGKACQLALEHLEEENTRVKALRDKLEKGILSSCPDCFV
                     NGDRRNRLPNTTNISFEFIEGESILMMLDQFGICASSGSACTSGSLEPSHVLRAMGVP
                     FTAAHGSIRFSLSRYNTEAEVDFVIEKMPGIVRRLRELSPFVKT"
     gene            complement(447..1520)
                     /gene="hydE"
                     /locus_tag="PLE88_09780"
     CDS             complement(447..1520)
                     /gene="hydE"
                     /locus_tag="PLE88_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOM76793.1"
                     /translation="MRERMTDISSSLSAEEIITWLRQTDEHKLESLWAAADAVRRQYV
                     GDAVHLRGLIEISNYCVRRCAYCGLSAENRQLVRYRMNADEIFSCAEEAAAYGYGTVV
                     MQAGEDYGIEADWLAGIIKRIKSHLPLAVTLSLGERSREELQQWREAGADRYLLRFET
                     SDRDLYNRIHPPLGPQSKNRFDILRQLKALGYETGSGVMIGIPGQTYHTLAKDIELFR
                     QLDLDMIGVGPYIPHPHTPLGRGEMDIRIDPNEQVPNTEQMTYKVIALSRLVCPQANI
                     PSTTALATVNRDSGRELGLMRGANIVMPNVTPAAYRINYEIYPGKACVNETAQQCQGC
                     LKARIAAIGRTVGRGPGSRRRCL"
     gene            complement(1505..2749)
                     /gene="hydF"
                     /locus_tag="PLE88_09785"
     CDS             complement(1505..2749)
                     /gene="hydF"
                     /locus_tag="PLE88_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOM76794.1"
                     /translation="MTQATPKGFRLHIGLFGRRNVGKSSLLNAIVRQQVSIVSDQAGT
                     TTDPVEKPMEFLPIGPVLFIDTAGIDDEGALGQLRIKKTRKTLDRVDLGVLVTDSNWT
                     EFETKLLSELIGRNVPVMAVFNKSDAALPNPEILEQLAKAKVPTVIVSCLKNEGIADF
                     RQALLETVPAEFIESPVIIGDIAGPGQTAVLVVPIDKEAPKGRLILPQVQTIRDLLDH
                     EAACMVVTDNQLRAALNNLKQPPRLVVTDSQAFSRVAAETPPEIMMTGFSILYSRLKG
                     DLVTQTLGAMAIERLKPGDAVLVAEACSHHPIGDDIGRVKIPNWLNHYVGGDLHFTTV
                     QGQDFPEDVSPYKLVIHCGACMWNRRTMLSRILQCRKAGVPITNYGLVIAYSLGIFER
                     ALQPFPAALEAYRNCRQKCVNE"
     gene            complement(2754..3518)
                     /locus_tag="PLE88_09790"
     CDS             complement(2754..3518)
                     /locus_tag="PLE88_09790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM76795.1"
                     /translation="MSSKQWYQKLFENYGRSYDSEPYINGTTGECDFIEREISCNKSV
                     KILDIGCGTGRHSIELAKRGYHVTGIDLSESMLAAAKEKAAKLGLTIDFMLRDARQLC
                     YTAEFGLAIMLCGGGFPLMETDEMNYQILRNATHAVKTGGKFIFTTLNGLFPLFHSVK
                     EYIASGGDAVSGGMDSSFDLLTFRYYTTFESIDDNGNKRIIKANERYYAPCEITWLLQ
                     SLGWSKIELFGAKLGAFSRNDKLTTEDFEMLVVAEK"
     gene            complement(3557..4957)
                     /gene="hydG"
                     /locus_tag="PLE88_09795"
     CDS             complement(3557..4957)
                     /gene="hydG"
                     /locus_tag="PLE88_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOM76796.1"
                     /translation="MADKTASIIKDEAIETTLKQTSSILDASAVYDILSKASQAKGLD
                     FYEVAVLSNISDPELLGELFNTAKRVKETIYGRRLVLFAPLYISNMCKNECLYCAFRA
                     KNKAIKRRALTQEEIAQEVKILENQGHKRILLVAGESYPKQGFQYILDAIETVYTTKS
                     GKGEIRRVNVNIAPLTVDEFRQLKAANIGTYQLFQETYHRQTYKSVHIGGKKTDYDWR
                     LEAIDRAMEAGIDDVGIGVLFGLCDWRFELLALMQHIRHLEERFGVGPHTISVPRLEP
                     ATGSDMASHPPKPVSDIDFRKIVAILRLAVPYTGIIMSTRETPKMRRETFALGVSQIS
                     AGSRTNPGGYTEGQDVLNEAQFCLGDHRSLDEVIYDAASLGYIPSFCTGCYRLGRTGQ
                     DFMDLAKPGLIKEHCDPNALSTFLEYLLDYGSDKTRHLGETLIANVLAEMDPVPRQRS
                     EAMLAKVRQGRRDVFC"
     gene            complement(5142..8258)
                     /locus_tag="PLE88_09800"
     CDS             complement(5142..8258)
                     /locus_tag="PLE88_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM76797.1"
                     /translation="MSEAFSSTEVRRGLIGWMTYNRITPNLIMLIFLIGGAILAFTIK
                     QEVFPEFDLDIVTVRVPYPGSSPEEVEQGIILSIEEAIRGLDGIKEITAVASEGFGTV
                     TAELLEGANQQKVYQDIKQEIDRITTFPEDAEEPEVSLLMRRREVLQIDLYGDVSEWV
                     LRSVAEQVRDSLLQSPGITQVELDGVREYEVLVLINQETLRTYGLTLQSVAAKIRASA
                     IELPGGYIETAGGDILLRVKERRDWAAEFARIPIITTAEGSILYLEDIAEVKDDFADT
                     DQAAFFNGYRAIGIEVYRIGKQTPIGVSQAARAAMARIETQLPPGVHWAINRDDADVY
                     RQRLELLLKNALCGLILVILLLGVFLEFKLAMWVTMGIPISFLGAFLFLPAMDVTINM
                     ISMFAFIIALGIVVDDAIVVGENIYEYRNRGMNYFQAAVLGARDVVMPVGFSIITNII
                     AFLPLYFIPGFIGKVWKVIPLVVITIFTISWIESTLILPSHLAHALPRSKNAVAVFLH
                     SRQQAFSRQFEWFIRTIFGRFLDFCIRYRQITVAASIAVFLIILSYILSGRIGMILMP
                     RVESDVSVVTAVLPFGSPMSKVKAVSDLLAQKAEGIIAENGGAFLSKGIYSSITENQA
                     QVRIYLTEPEVRPISTSRLTQLWREAVGQIPGLESLRFESDRGGLGSGAALTVELSHR
                     DITILDKASSELADLLSNFPNVKDIDDGYTPGKEQLNFKIKPEGQALGLTAQEIARQV
                     RNAFYGAEALRQQRGRNEIRVRVKLPLAQRRSEYDIETLLIRTPSGKDVPLMQAAEVA
                     RGRSYTSIQRRNGRRTVTVTANVEPIDDTNQVKETLESKILPNLLKDYPGLSYAWEGR
                     QQDLSEGMQSLNIGFLFALLGIYAMLAIPFRSYFQPIIVMIAIPFGIIGAIIGHLLMG
                     YSLSMMSMMGIVALSGVVVNDSLVLIDYANRLRSSENLPAIEAIHQAGVRRFRPILLT
                     TLTTFGGLAPMIFETSRQARFMIPMAISLGFGILFATGISLVLVPCLYIYLEDIKTII
                     KKFSNSFN"
     gene            complement(8255..9526)
                     /locus_tag="PLE88_09805"
     CDS             complement(8255..9526)
                     /locus_tag="PLE88_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM76798.1"
                     /translation="MNTSQSAPPGTPKHSPIWKNLGDIAIRYLIPAAIILLGIGFTKH
                     QMDTRPQAKLEKPALQARLVDVQTAQLTNITATVSAMGTVVPAKMTTLYPEVSGVVLS
                     LSEEVIPGGLIEAGQTLIQIDSKDYEAVVAQRQSELARAQLNLKLEYGNQTVARLEYK
                     LLEEALAEQDQELVLRKPHLEEAQAAVQAARAALDKAQLDVQRCTIKSPFNAIIQKKH
                     IDPGTRVSPSTPLLTLLGTDEYWIEILVPVHQLRWIAIPTENSQQGSRVMVFNNAGWG
                     EGIFREGRVLRLLGQLEEQGRMAQLLVSVSNPLGLSDNVSALPQLLIGSYVRVEIEGR
                     PIESVIEVRRDWLRDGNTVWIMNDQNRMEIRPVQIIFRNADAVYVTEGIKAGERIVTT
                     DISAPVEGMPLRLNNDPETAVLQTPDREAHL"
     gene            complement(9540..10952)
                     /locus_tag="PLE88_09810"
     CDS             complement(9540..10952)
                     /locus_tag="PLE88_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM76799.1"
                     /translation="MPIESIKKAVLLVWTAGLLGCAPIEPRGFKMPAAAPEAFSRDGT
                     EALCQRWWESLNDPNLNRLIETALENNFSIRSAWDRLSQAEQTARIAGAGLFPQAGYS
                     TQAQRSRTEASDIVNYSSAYSAGASISYELDFWGRIRSLRQAAVLDAQAAADDIQTAA
                     ITLSAAAAKTWYQLAEAKQQAGVIQKQLEINEKVLAIILTQFAQGLVGASDVLRQKQL
                     VESSRGQAVQNQETLVLLQHQLSVLCGEKPGLVWTDTPMELPEPAGLPAIGLPSDVLQ
                     RRPDVQKAYKAVQAADLRLAAAIADQYPSIGLFAGAETSAQQTKDLFEDWLANLAADI
                     TGPLFDAGLRKAEIARWRAAVSQKLNEYGQTILTALKETEDALAQEDYQRQYMRSLQE
                     QLRLARQVYERNRDKYLQGQMDYLRVLESLISLQSLERQELSARRILLERRIDLYRSI
                     AGSWPIDRPQQTAIVYEQFE"
     gene            complement(10991..11473)
                     /locus_tag="PLE88_09815"
     CDS             complement(10991..11473)
                     /locus_tag="PLE88_09815"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOM76800.1"
                     /translation="MLPWHNAFQKALDKYPLDGGLAPNAGPVERLKALIRSHLHRMLD
                     AGTLGHAGQILLMELANPTEAIELVKNDAISPLREHIRGIIRDLLGNEATDNKVNFCA
                     MSIIHLCFGIGLKRGKFPAFTASMSKESLFEALVEHITLFSLGGINAVKESQDVFEKS
                     "
BASE COUNT         2762 a         3059 c         2747 g         2950 t
ORIGIN      
        1 ccggggatta tcgctctggg caaggcctgc cagctggctc ttgaacatct cgaagaagaa
       61 aacacgcgcg ttaaggcact tcgggacaag ctggaaaaag gcattctcag ttcctgcccg
      121 gactgctttg tcaacggcga tcgccgcaat cgtcttccca ataccaccaa tatcagcttt
      181 gaattcattg agggggaatc tattctgatg atgcttgacc agttcggcat ctgcgccagt
      241 tccggttcgg cctgcacatc cggctcactg gagccgtcgc atgtccttcg tgcgatgggt
      301 gtgcctttta ctgctgctca cggctctatc cgattcagct tgagccgcta taataccgaa
      361 gccgaagtcg attttgttat agaaaagatg cccggcattg tcagacgcct tcgggaactg
      421 agcccgtttg taaaaacctg attgtgttac agacatcgcc tccgggagcc ggggccgcgg
      481 cccactgttc ttccgatggc agcgatacgg gctttaaggc atccttgaca ttgctgggcg
      541 gtttcattca cacaggcctt gccgggataa atttcgtagt taatgcggta tgctgcgggg
      601 gtaacgttcg gcatcacaat attggcccct cgcatcaacc caagttctct gccgctgtcg
      661 cggttgacgg ttgccagtgc cgtcgtgctg ggtatattgg cctgcggaca gactaagcgg
      721 ctgagggcaa taactttgta ggtcatctgt tcggtattgg ggacctgctc atttgggtcg
      781 atacggatgt ccatttcgcc ccgtcccagc ggtgtatgcg ggtgcgggat atagggtcca
      841 acgccaatca tatccagatc cagctgccgg aataattcaa tatccttggc cagcgtatgg
      901 tatgtttgtc cgggtatgcc gatcatgacg cctgagcctg tttcgtaccc taacgccttc
      961 agctgccgca gaatatcaaa gcggttcttg gattggggcc ccagcggtgg gtgtatccgg
     1021 ttatacagat ctctgtcgga ggtttcaaac cgcagaagat aacggtcagc tccggcctct
     1081 ctccattgct gcagctcttc ccgtgagcgt tcgcccagac tcagcgttac agccagcggc
     1141 aggtgtgatt taatgcgttt aataatgcca gccagccaat cggcctctat gccgtaatcc
     1201 tcgccggcct gcatcacgac cgtaccgtat ccataggcgg ctgcttcttc tgcacaggaa
     1261 aagatttcat ctgcattcat tcgatagcgc acaagctgcc tgttttctgc tgataagccg
     1321 cagtaagcac agcggcgcac acaatagttg ctgatttcaa tcagccctcg cagatgcacc
     1381 gcatctccta catattggcg gcgcacggca tcggcagctg cccacagtga ttccagctta
     1441 tgttcatctg tttgacgcag ccatgttatg atttcttcgg ccgacaatga agaagaaata
     1501 tctgtcattc gttcacgcat ttttggcggc agtttctgta ggcttccagc gccgccggaa
     1561 aaggctgcaa tgcccgctca aaaataccca gactgtatgc gataaccaga ccataattgg
     1621 taatcggaac acctgccttg cggcattgca gaatccggct cagcattgtc cgccgattcc
     1681 acatgcatgc cccgcaatgg ataaccagct tgtaggggga tacatcttct ggaaaatcct
     1741 gcccctgaac ggttgtaaag tgaaggtcgc ccccgacata gtgattcagc cagttaggga
     1801 ttttaacacg tccaatatcg tctcctatcg gatgatgact gcatgcctca gccaccagca
     1861 cggcatcccc cggcttaaga cgttcgattg ccatagcccc gagcgtttgg gtcacaaggt
     1921 cgcctttcag acgcgaataa agaatcgaaa aacctgtcat cattatctcc ggcggtgtct
     1981 ccgccgcaac ccttgaaaag gcctgtgaat cggttacaac cagccgaggc ggctgtttga
     2041 ggttgttcag ggccgcacgc agctggttat ctgtaacaac catacacgcc gcttcgtgat
     2101 ccagcagatc gcgaatggtc tggacctgcg gaagaatcag ccggccttta ggagcttcct
     2161 tgtcaatcgg tacaacaaga acggccgtct gacccggccc ggcaatatca ccgataataa
     2221 ctgggctttc aatgaattcg gccggaacgg tctccagaag cgcctgtcga aaatccgcaa
     2281 tgccttcatt tttcagacaa gatacgataa ccgttggcac tttggctttt gccaattgct
     2341 ccagaatctc tggattgggc aaagccgcat cacttttatt aaatacagcc ataacaggga
     2401 cattgcgtcc gattagttcg ctcagcagtt ttgtttcgaa ttccgtccag ttgctgtcgg
     2461 taacgagtac tcctaaatcg acccggtcaa gcgtcttgcg ggttttcttg atgcgaagct
     2521 gtcccaatgc tccctcatca tcaatgccgg ctgtatcgat aaatagtaca ggcccgatgg
     2581 gcagaaactc cattggtttt tcaaccggat ctgtagtggt gccggcttgg tcggagacga
     2641 ttgaaacctg ctgacgcacg atggcattca gcagacttga cttgcccaca tttcgcctgc
     2701 caaacagccc gatatgcagt ctgaatcctt tgggagttgc ctgtgtcatc gttttatttc
     2761 tctgctacaa ccagcatttc gaaatcttcg gttgtcagtt tgtcatttcg ggaaaaagcc
     2821 cccagtttag ctccgaatag ttctattttt gaccatccga gagactgaag gagccaggta
     2881 atttcgcagg gcgcatagta gcgttcattg gcttttatga tccgtttatt gccgttatcg
     2941 tcgatactct caaatgtggt ataatatcgg aaagtcaaaa ggtcaaaact gctgtccatc
     3001 ccgccgctta ctgcatcgcc gccggaagcg atatactctt tgaccgaatg aaacagagga
     3061 aacaggccgt tgagagtagt aaagatgaac ttgccgccgg ttttgacagc atgcgttgca
     3121 tttctgagta tctggtagtt catttcgtct gtttccatca aggggaaacc acccccgcaa
     3181 agcataatag ccagcccgaa ctcggctgta tagcaaagct gccgtgcatc acggagcata
     3241 aaatcgattg tcagccccag cttggcggct ttctcttttg ctgccgcaag catcgattcg
     3301 gacaaatcaa tccctgttac gtgataaccg cgtttggcaa gttcgatgct gtgccgtcca
     3361 gtcccgcagc cgatatcgag aatttttaca gacttattgc agctgatttc ccgttcaata
     3421 aaatcgcatt cgcctgttgt tccgttaata tacggttcgc tgtcgtagga gcgcccgtag
     3481 ttttcaaaca gtttttgata ccattgcttg ctgctcattt cagcctcttg cgtccttgtt
     3541 ataaactgct gaataatcag caaaaaacat cccgtcgccc ttgacgaacc ttggccagca
     3601 ttgcctcgct gcgttgtctg ggtactggat ccatctcagc cagcacgttg gcaattagag
     3661 tttctcccaa atggcgagtc ttgtctgaac cgtaatccag caggtattca agaaatgttg
     3721 aaagggcatt tgggtcacag tgttctttaa taagtcccgg cttagctaaa tccataaaat
     3781 cctgaccggt acggccgagg cggtagcatc ctgtacaaaa cgaagggata taacccagac
     3841 ttgcagcatc gtaaataacc tcatccagcg agcggtgatc gccgagacag aattgtgcct
     3901 cattcagtac atcctgacct tctgtatagc cgcccggatt tgttcggctg ccggcggaga
     3961 tctgagaaac tcccaatgca aaggtttccc gccgcatctt cggggtttca cgggtagaca
     4021 taataatgcc tgtataaggt acggccagac gcagaatcgc cacaatcttg cgaaaatcga
     4081 tatcactgac tggcttaggc gggtgggatg ccatgtctga gcctgtcgcc ggctccagcc
     4141 gaggcacgct gatggtatgc ggaccaactc cgaaccgttc ttccagatgg cgaatatgct
     4201 gcatcaaagc cagcagttca aaccgccagt cgcatagtcc gaacagcacg cctatgccca
     4261 catcgtcaat gcctgcttcc attgcgcggt caatggcttc cagccgccag tcataatctg
     4321 tctttttgcc ccctatatgc acgcttttat aagtttgccg gtggtatgtt tcctgaaata
     4381 gctggtaagt tccaatattg gcggctttca gctgtcgaaa ttcatccact gtcagcgggg
     4441 caatattgac attgacccgg cggatttcac ctttgccgct tttggttgtg taaacggttt
     4501 caatagcatc caatatgtat tgaaagccct gcttgggata cgattcacct gcaaccagca
     4561 aaatgcgttt gtggccttga ttttcgagga tttttacttc ttgggcgatt tcttcctgtg
     4621 ttaatgcccg ccgtttgatg gccttatttt tggcccgaaa agcgcaataa aggcattcat
     4681 tcttgcacat attagagata tacagcgggg caaacaaaac caaccgccgc ccgtaaatgg
     4741 tctctttgac acgcttggcg gtattaaaca gctcgcccag tagctccgga tcgctaatgt
     4801 tgcttagaac agcgacttca taaaaatcca atccttttgc ctgagaagcc tttgaaagaa
     4861 tatcatatac agccgaagca tccagtatag aggaggtttg tttgagggtc gtttcgatgg
     4921 cctcatcttt gatgattgat gcagttttat ctgccatttt atattttcct tgatacgaaa
     4981 caggacatcc tgcttttgct tgaaaacagg aaaattttta tgcataaaaa agaacgttaa
     5041 cagaataaat atatgtaata aactaataaa tgtcaataaa atatgtgaaa ataataacaa
     5101 taaaacagaa agatttatag tacaaagcag gtcttgtatg tctaattgaa agaatttgag
     5161 aatttcttta ttatggtttt aatatcttcg aggtagatgt aaagacaggg aacaagcacc
     5221 aacgagattc ctgtggcaaa aagaataccg aaccccaatg aaattgccat tggaatcata
     5281 aagcgtgcct gccgcgatgt ttcaaaaatc atcggtgcta atcctccaaa agtcgtcagt
     5341 gtcgtcagca gaataggacg aaaccgccgg accccggctt gatggatcgc ctctatagca
     5401 ggcaagtttt cgctgctgcg cagacggttg gcgtaatcaa tcaacaccaa agaatcgtta
     5461 accaccacgc cggacagtgc cacgatgccc atcatactca tcatactaag cgaatagccc
     5521 atcagcagat gaccgataat ggctccaata atgccgaagg ggattgcaat catcacgata
     5581 attggctgaa agtagcttct gaaaggaatg gccagcattg cataaatacc cagcaaagca
     5641 aatagaaagc cgatattaag gctttgcatt ccttcgctca gatcctgctg ccgtccttcc
     5701 catgcatagc ttaagccggg ataatctttt aacagattcg gcagaatttt gctttccagc
     5761 gtttccttga cctgattggt gtcatcaatc ggttcgacat tagcggttac ggttacggta
     5821 cgccggccgt tccgccgctg aatgctcgta taggaacggc cgcgtgcaac ttctgctgcc
     5881 tgcatgagag ggacatcttt acccgatgga gtccgtatca gcagagtttc tatatcatat
     5941 tcacttctgc gctgggcaag cggaagcttt acacgaaccc gtatttcgtt gcgtccacgc
     6001 tgctgccgca gggcttcagc gccatagaag gcattgcgca cctgacgggc gatttcttgg
     6061 gctgttaacc ccagtgcctg cccttcaggc ttgattttga aattcagctg ttccttgccg
     6121 ggcgtatagc catcgtcgat atctttgaca ttcggaaaat tgctcagcaa atcagccaat
     6181 tcagagctgg ctttatccag aatagtgata tcccgatgac ttaattcgac agttaaagcg
     6241 gctcctgaac ctaatccgcc gcggtcggat tcaaaccgca gcgattcaag acccggtatc
     6301 tgcccgacgg cttcacgcca aagctgtgtc agccggcttg tactgattgg acggacttcc
     6361 ggttcggtca ggtagatacg cacctgtgcc tggttttcag taatactgct gtaaatgccc
     6421 ttgcttaaaa acgctccgcc gttttcagca ataatgcctt cggctttttg tgccagcagg
     6481 tcgctgacag ccttgacttt tgacattgga ctgccgaatg gaagaacagc tgttaccacc
     6541 gagacatcgg attcaacacg aggcattaaa atcataccaa tgcgtccgct taagatatag
     6601 ctgagaataa tcaggaatac cgcaatcgat gcagcaaccg taatctgccg gtaccgaatg
     6661 cagaaatcca gaaaacggcc gaaaatagtg cgaatgaacc attcaaactg ccggctgaag
     6721 gcctgctgcc ggctgtgcag aaaaactgcg acagcgtttt tactgcgggg cagggcgtgg
     6781 gctaagtgtg aggggagaat caaggtggat tctatccaag agattgtaaa aatcgtgatg
     6841 accaccaaag ggataacttt ccagactttc ccgataaatc ccggaataaa ataaagcggc
     6901 agaaaagcga taatgttggt aataatgctg aatcccaccg gcattaccac atcgcgtgcg
     6961 cccagaacag ccgcttggaa atagttcatc ccgcgattcc ggtattcata gatgttttcg
     7021 ccgacaacaa tggcgtcatc caccacgatt cccagtgcaa tgataaatgc aaacatcgag
     7081 atcatattga tggtaacgtc cattgccggc agaaacaaaa acgctcctaa gaaagaaatg
     7141 ggaatcccca tcgtaaccca catcgccaat ttaaattcaa gaaaaacacc cagcaagagt
     7201 atcaccagaa tcagaccgca caaggcattc ttcagcagca gttccagcct ctggcgatac
     7261 acatcagcgt catccctgtt gatagcccag tgaactcccg ggggcagctg tgtttcaatc
     7321 cgcgccattg ctgcacgggc tgcctgggaa acgccgatag gcgtttgctt gccgatacga
     7381 tagacttcga tgccgatggc tcggtatccg ttaaaaaatg cggcctgatc ggtatctgca
     7441 aagtcatctt taacctcagc aatatcttcc agatagagaa tggagccctc tgctgtggtg
     7501 ataattggaa ttcgcgcaaa ttcagctgcc cagtcgcggc gttccttgac gcgaagaagg
     7561 atatctccgc cggcagtttc aatgtatcct ccgggcaatt caatcgcact ggccctgatt
     7621 ttggcggcta ccgactgcag cgtcaatccg taggtccgca atgtttcctg attaatcagt
     7681 acaagtactt catactcccg cacaccatcc agctcaacct gtgtgatccc cggactttga
     7741 agcaaactgt cccgaacctg ttcagccaca ctccgcagca cccattccga gacatccccg
     7801 tataaatcaa tctgcaggac ttcccgccgg cgcataagaa gacttacttc cggctcttcc
     7861 gcatcctccg gaaaagtagt aatccggtca atctcctgct tgatatcctg ataaaccttc
     7921 tgctggttgg caccttccag cagttcagct gtgacagttc caaaaccttc ggatgcgaca
     7981 gcagttatct ccttaatacc gtccaatccc cgaattgcct cttcaatcga tagaataatg
     8041 ccttgttcga cttcttccgg gctggacccc ggatagggta cacggacggt tactatatca
     8101 aggtcaaact cgggaaaaac ttcctgctta atggtaaagg caagaattgc accgccaatc
     8161 aggaaaatca gcataatcag attcggcgtg atgcggttat aggtcatcca cccaatcagc
     8221 ccgcgccgga cttcggtgct tgaaaatgct tcgctcacag gtgggcctcc ctgtctgggg
     8281 tttgcaaaac agcagtttca ggatcattgt tcagccggag cggcattcct tctaccggtg
     8341 ctgaaatatc tgtggtaaca attcgctctc cggccttgat tccttctgtg acataaacag
     8401 catccgcatt ccggaaaata atctgcaccg gccggatttc cattctattc tggtcattca
     8461 taatccagac cgtattgccg tcccgcagcc agtcccgcct gacttcaatt accgattcaa
     8521 tcggccgtcc ttcaatttca acccgcacat acgagccgat cagcagctgc ggcagtgccg
     8581 atacgttgtc tgacagtccc agcggattgc tgacggaaac cagcagctgg gccattcgtc
     8641 cctgttcttc taattgcccc agcaggcgaa gaactctgcc ttcccgaaaa atcccttctc
     8701 cccaaccggc attgttgaat accatgactc gtgaaccctg ttgagaattt tccgtcggaa
     8761 ttgctatcca gcgaagctga tgaactggaa caaggatttc aatccagtat tcatctgttc
     8821 ccagcagcgt cagcaggggg gtggacggcg aaacccgtgt gcccggatcg atgtgctttt
     8881 tctggataat ggcgttaaac ggcgatttga ttgtgcaccg ctgcacatcc aattgtgctt
     8941 tatcaagagc cgcccgggcc gcttggacag cggcttgtgc ctcctccaaa tgcggcttgc
     9001 ggagcaccaa ttcctggtcc tgttctgcta aggcttcttc cagcagttta tattccagtc
     9061 gggcaacagt ctggttgccg tattccaatt tcagattcag ctgtgccctt gccagttcac
     9121 tttgccgctg ggctacgaca gcttcgtaat cttttgaatc gatttgaatc agtgtttgtc
     9181 cggcttcaat aaggcctccc ggtataacct cctcgctgag cgaaagaacc acgcccgaca
     9241 cttccggata aagtgtggtc atttttgccg ggacgacagt gcccatcgca gatactgtag
     9301 ccgtgatgtt tgtcagctgc gcagtctgga catcaaccag ccgtgcctgc agggcgggtt
     9361 tttccagttt tgcctgcgga cgggtgtcca tctggtgttt ggtaaatccg atgcccaaaa
     9421 gtataatggc cgcaggaatc agatagcgga tggcaatgtc tcctaaattc ttccagattg
     9481 gcgaatgctt cggcgttccg ggcggcgctg attgagaagt attcatcgtg tatttcctct
     9541 cattcaaatt gctcatagac aatggctgtc tgttggggtc tatctatagg ccagctgccg
     9601 gcgattgagc ggtataagtc gattctccgt tccagcaaaa tacgccttgc cgacaactcc
     9661 tgccgttcca agctttgcag cgatatcagt gattcgagta cacgcagata atccatctga
     9721 ccttgaaggt atttatcgcg gtttctttcg tatacctgcc gggccagccg tagctgttcc
     9781 tgcagactcc gcatgtattg gcgctgatag tcttcctgtg ccaacgcatc ttctgtttct
     9841 tttaatgccg tcaggattgt ctgcccgtat tcgttaagtt tctgcgaaac cgctgcccgc
     9901 cagcgtgcaa tttctgcttt gcggagaccc gcatcgaaca gcggccctgt gatgtcggct
     9961 gccagattgg ccagccagtc ttcaaacagg tctttcgttt gttgggcaga tgtttcagcg
    10021 ccggcgaaca acccgataga cggatactgg tcggcaatag cggcagccag ccgcaagtct
    10081 gccgcttgta ccgccttgta agctttttgc acatcaggcc gtctctgtaa aacatccgac
    10141 ggaagtccta tcgccggcag accggccggt tccggcagct ccattggcgt gtctgtccat
    10201 accagaccgg gtttttcccc gcacagtaca gacaattgat gctgcaataa aaccagtgtt
    10261 tcctgatttt gaacagcttg tccgcggctg gattcaacca gctgtttttg ccgcagcaca
    10321 tctgaagccc cgactagacc ctgtgcaaac tgggttaaga taatagccag cactttttca
    10381 ttaatctcca gctgtttttg aatgacgcca gcctgctgct tggcctctgc cagctgatac
    10441 catgtcttgg cagcggcggc actgagcgta attgccgctg tctgtatgtc atctgctgct
    10501 gcctgggcat ccaaaaccgc tgcctgacgc aaggagcgaa tgcgccccca aaaatccaat
    10561 tcgtatgaaa tagatgcccc tgctgaatac gctgaagaat aattcacaat atcgcttgct
    10621 tcagtccggc tgcgctgtgc ttgtgtgctg taaccggctt gggggaaaag gccggctccg
    10681 gcaatccggg ctgtctgctc ggcctgactg agacgatccc acgctgagcg gatggaaaaa
    10741 ttattctcca atgccgtttc tattaggcgg ttcagatttg ggtcattcag cgattcccac
    10801 cagcgctggc acagggcttc ggttccatca cgggaaaacg cttccggcgc agcggccggc
    10861 attttgaaac ctcgcggctc aataggcgca catcccagta gcccagccgt ccacacaagc
    10921 aatacggctt tctttatgct ctcaatcggc acaatctatt cctccgtcag acagaggcaa
    10981 actataattg ttatgatttc tcaaatacat cttgactctc tttcacagca ttaatgcctc
    11041 ccaaggaaaa gagggtgata tgctccacaa gggcttcaaa aagcgattct ttactcatgg
    11101 aagctgtaaa agcaggaaat ttcccgcgtt tgaggccaat gccgaaacag agatggataa
    11161 tgctcatggc acaaaagttg accttattgt ctgtggcctc atttcctagc aaatcccgaa
    11221 ttatgccgcg aatatgttcg cggaggggac tgatggcatc atttttgacc agttcaatcg
    11281 cctcggtcgg attggccaat tccatcagga gaatctggcc ggcatgcccc agcgttcccg
    11341 catccagcat ccgatgcaaa tgcgagcgga taagtgcttt cagccgctca acaggtccgg
    11401 cattgggggc gagccctcca tccagcgggt atttgtcaag agctttttga aaggcattgt
    11461 gccagggtaa catcccccat ttttaggtcc attgactaag ttaggatttc cctgtaaa
//
LOCUS       DAOTBO010000088        11459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_4810_length_11459_cov_6.248860, whole genome shotgun sequence.
ACCESSION   DAOTBO010000088 DAOTBO010000000
VERSION     DAOTBO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4810_length_11459_cov_6.248860"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLE88_09820"
     CDS             complement(<1..688)
                     /locus_tag="PLE88_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM76801.1"
                     /translation="MNPLYIGATLRDCGKTSVSLGLMQLLQARGINPGYCKPVGQHYV
                     RYHDKNIDEDGVLIHQVFNLQDEPYFLSPIAIERDFTRKFIQNPNVKPLEEEILRCHQ
                     ELLKKHPMVIVEGTGHAGVGSCFGLSNARVAQLLGAKVVIVTSGGIGRPIDEIAMSLA
                     LFRDFNVDVLGVILNKILPEKYDMVKDTVDKGLRLLGTRLVGAIPFDRHLTTFTVGQL
                     AEEFNYQIICG"
     gene            complement(764..1234)
                     /locus_tag="PLE88_09825"
     CDS             complement(764..1234)
                     /locus_tag="PLE88_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOM76802.1"
                     /translation="MVVGLGNPLMGDEGIGIVLIEKLAQMAAEGRLPADSVDYHDGGT
                     GGINLLHAIADRRKAVILDCCRMNTAPGTIKRFTPDDVVTVKKMAHLSLHETDILQVI
                     DLAQQLGQCPEEIVFFGIEPETVELQTALSPTLAAQMPAYLALIQNELLAQRHL"
     gene            complement(1455..2072)
                     /locus_tag="PLE88_09830"
     CDS             complement(1455..2072)
                     /locus_tag="PLE88_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76803.1"
                     /translation="MKKLILFTAILCFTSGAFADPDIQFSPYGPTGWSYNGNYTFTFG
                     PQDIDFVLGGQTDALFGQLVYLPDLTLSSYSSIIPGVAGTGMFAGGGVLQIKDTAGNL
                     LAAGVLEEGSYAAVFTTSSAYPEVALDVTITQINNTIGSAYLSTLSVGMKWDLNLSLQ
                     WNGSFDAMITSNGTGSDGMSGSLTIPEPATLVLLGLGALLSGRRK"
     gene            complement(2300..2947)
                     /locus_tag="PLE88_09835"
     CDS             complement(2300..2947)
                     /locus_tag="PLE88_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76804.1"
                     /translation="MMKKMGLIACLCVAATVFAVPDIEFSPGGSVPGNWKYTGLTPSS
                     GIFSFTQQIDIDAIQGSTADALYDQFVFLPDLTLSNYVSAFPGVGSGIVTAGGPVEIK
                     DGLGNVLVSGTLAEGNYYAVFATSVIYPEGSDVVLDIQINYINNTIGSAYLDTLKVGN
                     YFDLNLSLQWSANFDTMIQKVASGQNGFSGSLSYAVPEPATMLLLAAGGLLLRKR"
     gene            complement(3270..4088)
                     /locus_tag="PLE88_09840"
     CDS             complement(3270..4088)
                     /locus_tag="PLE88_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76805.1"
                     /translation="MRRLVLFLMIVSLNFSAGAVLVFSDDFEDGNRSGWYGFSDLDSA
                     SPLTVKKDTGAGTLNSGYALFLTNPATYCSYWGAANINTIQLVKPGDYIRLSIDLRLD
                     GVIDSANGLRFGVFNSAGTPITYDSNGYAKSKDDAGYFMRISTGKYTDARITFEPGSD
                     SFFMIGADMVQLYNSSLTFNDANKHHFDFDVKLTKDGGVEISLYVDGSFWASAQHSQS
                     VVTTFNEIGFCSNKSGGVDFAIDNIAVAYIPEPATMVLGALGGLICLAGRKQTT"
     gene            complement(4338..5036)
                     /locus_tag="PLE88_09845"
     CDS             complement(4338..5036)
                     /locus_tag="PLE88_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76806.1"
                     /translation="MKRLMCIAISVMAVTAVNANLVQLYLDTAPNASGSPLWPAFRAA
                     AEAAIYNGTFVNQANSNDSGNVGTLNYSVDDYMVYSFGDLGNRLHAFYYIPGETISSL
                     SGRFQVSIEYEYDGVWYNPYEEYGWGKWVTPGSWINYDGNGDSVIDGVMGSMGNALWG
                     AYGYSSDTPEAQAALAADLQDAENYLGNTRFLARLDSVTYELTALHTPAIPEPATLIL
                     LGLGGLFLGKFKKA"
     assembly_gap    5320..5419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5420..5615
                     /locus_tag="PLE88_09850"
     CDS             <5420..5615
                     /locus_tag="PLE88_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76807.1"
                     /translation="WRFWYISRRRLNRQQLKAILSFLWPQKLFVQLPASSNPLSAFGF
                     TVGPTQNRPVILTPALLCDL"
     gene            complement(5618..6160)
                     /locus_tag="PLE88_09855"
     CDS             complement(5618..6160)
                     /locus_tag="PLE88_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM76808.1"
                     /translation="MNRVTVFLVAALAAAAVVPAEAGLKIYVNLGNGLWADYPDSKIT
                     IRPSDVIKIGIIDVDGTGGTGSMALGIAVGAASGSLDISQAVLSQGVSAELTAGGAEQ
                     VGMQSPFVSISNITPQGAGGWLIQDTLFHCDGPGDVTIALVDNNGNIIDAQVIHQTPE
                     PATMLLLGLGGLFFMKVKIG"
     assembly_gap    6590..6689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6690..6885
                     /locus_tag="PLE88_09860"
     CDS             <6690..6885
                     /locus_tag="PLE88_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76809.1"
                     /translation="QLLKEPHYSIAALAAVIVLPGSTARSPAAKPSGARGDEALARKE
                     RDEEVLFSFFCYSVVKRTAL"
     gene            complement(7023..7421)
                     /locus_tag="PLE88_09865"
     CDS             complement(7023..7421)
                     /locus_tag="PLE88_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76810.1"
                     /translation="MCQIYSTILIVAAQDKAQALQELPLRLLVMDTGKEAIRCLREEQ
                     IDTVVSRWNVVDMPDGMLLEKITAARAKIPTVAFIEPGNMEQEVVARSLGVNVILSDD
                     IDDDYFRSTVCQILHIEDIAAVTVAAGQMQ"
     gene            complement(7708..10677)
                     /locus_tag="PLE88_09870"
     CDS             complement(7708..10677)
                     /locus_tag="PLE88_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOM76811.1"
                     /translation="MRQWGHCYIFTVSVLFLSLSGHLYGKIIYVNSNAGGSQDGTSWH
                     NGFLTITAAINAADYNDQIWIKEGVYQERIHIPDGVALYGGFAGTETDLSQRDYNTYM
                     SIIDAQYTGITVTIDNSADSTTRLDGCAVIRGYGIHGGGIKIIGSAPTIVNNLIIYNK
                     TDGAGAGISVWGFKVVPPVEVPYIGSNVIVENRSGNDEGDGGGIAVIGSSPFIIGNTF
                     LRNEATRNGGAIACWRSSQPLIAYNYIFANSASTPVNYYTDYITETDGGGAIFASSTD
                     LDGWPIPDSVAAPEIYNNVIAANAAFKGGGICVINSIRTDLGVAEIINNTIVSNQGAG
                     IYWSNTTPTIVNNLIAYNTIGLHQYVFGLTGCDIRANCVYGNQTQNDTLDYVGLSSQT
                     GIDGNISAAPLLADYRFGKLHLQPGSPCIDAGLSAVIDANWTDDIDGQDRIQGGSVDI
                     GADESDGTLWDTDHPIIRVKPDGNDEADGQSWATAKKTIGRALAAVQNSGGEIWAAAG
                     TYPERINLSAYVYLYGGFAGNETERSQRNVVANPTILDGTGTPTIVTILNAGYLVCAL
                     DGWTVQNGGVYTDGNPFAVVGVQGIGGGIYMRVASPLIENNTIQYNSLGTPYAAYFAR
                     GGGIACFMGYPIIRDNVIRWNETLTFDSWGGGIYCNLSGPWIENNLITQNHCGRGAAI
                     YGYVSTPLIKGNTIIGNQMYALPPVYMGSPEGAVDLNLCPAFVIENNHFKNNQASVGA
                     AITLASSDWGIIRNNLFIDNIAYDVSSMAGGSGGGIYCINPQNPTGTLSILNNTFSGN
                     TATHLFYGERGGAIALQLLSENVLIANNVMAFNSSGIWLDSSRPYLPTLFSNCMYNTG
                     SNYVNLPAGAGDIMQDPGFVNRTAGDYRLRHDSICINAANPNLADIGSADMDGQPRIR
                     YGRADIGAFEVFPIAGDFEPDEDVDIADLAWIAARWLQTSCTCPGWCGGADLNKDGTV
                     GLADIVLAADHWLAHKF"
     gene            complement(10974..>11459)
                     /locus_tag="PLE88_09875"
     rRNA            complement(10974..>11459)
                     /locus_tag="PLE88_09875"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2774 a         2954 c         2672 g         2859 t
ORIGIN      
        1 ctccgcagat aatctggtag ttgaattctt ccgcaagctg accaaccgtg aaggttgtca
       61 gatggcggtc gaaaggaatt gcaccaacca ggcgcgtccc gagcagccga agccccttat
      121 ctacggtgtc cttgaccata tcgtattttt caggcaggat tttattgaga atgactccca
      181 gcacgtcgac attaaaatct ctgaacaggg ccaagctcat cgcgatttca tcaatgggcc
      241 gtccgatgcc gcccgaagta acgataacaa ccttggcgcc aagaagctgg gctacccgtg
      301 cattcgacag tccaaaacag cttcctacgc cagcatggcc ggttccttcc acaataacca
      361 tcggatgctt tttcagaagc tcctgatgac accggagaat ttcctcttcc agcggcttga
      421 cattcggatt ctggataaat tttcgggtaa aatccctttc gatggcaata gggctgagaa
      481 agtagggttc atcttgaaga ttaaagacct gatgtatcag cacgccgtct tcatcgatat
      541 ttttatcatg gtagcgcacg tagtgctggc caacaggttt gcagtagccc ggattaatcc
      601 cgcgggcctg aagaagttgc atcagaccca gactgaccga ggttttgccg cagtcgcgta
      661 acgtcgcgcc gatatagagc ggattcatag gcatatctgt atttagagtc ttaaatagtt
      721 tgcgttatta tacagcaaaa tgagatgcaa ttctactgga aaattacaaa tgcctctgcg
      781 caagcaattc attctggata agagcaagat acgcaggcat ttgggcagcc agtgtcgggc
      841 tgagtgccgt ttgcagttca acggtttccg gttcgatgcc gaaaaagaca atctcctccg
      901 gacattgccc cagctgctgg gctaaatcga taacttggag aatatctgtc tcgtggaggg
      961 ataaatgagc catttttttt acggttacca catcatcggg cgtaaaccgc ttaattgttc
     1021 ccggcgccgt attcatacgg cagcagtcaa ggataacagc tttgcggcgg tcggcaatag
     1081 catgcaggag attgatgccg ccggtgccgc cgtcgtgata gtccacagaa tccgccggca
     1141 gcctgccttc tgcggccatt tgcgccagct tttcaatcag cacaatcccg ataccctcat
     1201 cgcccatcag cggattgcca agcccgacta caatggttga tgctctgata ctctcatctc
     1261 ccagaaaaag cttatttgag gagcactcct acaccgcaga cattctaacc aaaaggcccg
     1321 cgggtaaaaa taaaaaaacc ggattttcct tctgaaaaac ccggtctttt cgagggtgca
     1381 ttgaagctgt ccctcagacg agaaaccgat tctcgcctca tcctccgatg atgaagcttg
     1441 tcagtaaaaa ggtcttattt tctgcggccg gataaaagtg cgcctaatcc cagcaggacc
     1501 agcgtagccg gttccgggat tgtcaagctg ccgctcattc cgtcagaacc ggttccgttt
     1561 gaggttatca tggcatcaaa gctcccgttc cactgcagcg acaaattcaa atcccacttc
     1621 attccaaccg acagcgtgct caagtaagca gatccaatcg tattgttaat ctgcgtaata
     1681 gtaacatcca gagcaacttc aggataagcg gaagacgttg tgaaaacagc cgcataactg
     1741 ccttcttcaa gtactcctgc tgccagcagg ttccccgcag tgtccttaat ttggagaacg
     1801 ccgccgccgg caaacatgcc ggtgcctgct acacccggta taatcgagct gtagctggaa
     1861 agcgtcaagt ccggcagata caccagctgc ccgaacaaag cgtccgtctg cccgcccaga
     1921 acaaaatcaa tgtcctgcgg tccaaaagta aatgtgtagt ttccattata ggaccatcct
     1981 gtcgggccgt aaggcgaaaa ctggatatcc ggatcggcaa atgcaccaga ggtgaaacac
     2041 agaattgcgg taaacaagat caactttttc atctttcctc tcctaactat cttccccact
     2101 ctgcaggatg caaagtcaat caggacaatc ctgttaaccg ctatcctgct taatgccaat
     2161 gattatatcc gaccagcgga ctattaatca atcgtggttt ttacggattt ttacaaaaaa
     2221 tcagtttttc agtactcctt atatacaaaa aaagccccga tttttgtcgg ggcttttgaa
     2281 aatacaaaac ttttcccaat cagcgctttc ttaaaagaag cccgcccgct gccagcagca
     2341 gcattgttgc cggctcgggt actgcatagg acaacgatcc gctgaagccg ttttgtccgc
     2401 ttgcgacctt ctgaatcatc gtgtcaaaat tagccgacca ttgcagcgac agattcaggt
     2461 cgaaataatt gccgactttc aatgtatcca gataagcgga gccgatggtg ttattgatat
     2521 agttgatttg aatatccaaa acaacatccg acccttccgg atagataacc gatgtggcaa
     2581 aaacagcata gtagttgcct tcggccagcg tgccggaaac caaaacattg cccaatccgt
     2641 ccttaatttc aacaggaccg ccggctgtta caatgccgga acctacaccc gggaaagctg
     2701 aaacgtaatt gctcagtgtt agatcaggca ggaagacgaa ctgatcataa agcgcatcag
     2761 ccgtactgcc ctgaattgca tcgatatcaa tctgctgggt aaaactgaat atccccgagc
     2821 tgggcgtcag ccctgtgtac ttccaatttc cgggtacact gccgcccggt gaaaattcaa
     2881 tatcaggaac tgcaaatacc gttgcggcga cacacagaca tgcaataagc cccatcttct
     2941 tcatcatctt tcctctccac gcaaattgtt caactggttt ctattctgga aacagatata
     3001 gtactttact ttatctgccc cgccatcttt ccatccttat tatactctta aaggtgctct
     3061 gttcaatcgt ggtttctacg ggatttttaa aaatcggcgt tttcagcatt aaaaatgctg
     3121 tttttttaag taaaaaattt gcttttgcat aatttttttg tatatgcgga ttcctcctgt
     3181 gtccggtttt tgtccgaaaa actgtaagaa ttgaaaaaaa gcccggctta aaaatcgaag
     3241 ccgggctttt tgtagatata caagatgcat taagttgtct gttttctgcc ggccagacat
     3301 attaatccgc ccagtgctcc caagaccatc gttgccggct cgggaatata agctacagca
     3361 atattgtcaa tcgcaaaatc aacacctcca cttttattgc tgcaaaatcc aatttcattg
     3421 aatgtcgtaa caacagattg actgtgctgc gcgcttgccc aaaatgatcc gtcaacgtac
     3481 agtgaaattt caacaccgcc atcttttgta agttttacat caaaatcaaa gtgatgttta
     3541 ttggcatcgt tgaaagtcag agaactgtta tagagttgca ccatatcagc accgatcata
     3601 aaaaaggaat ctgagccggg ctcaaaagtg attcttgcat cagtgtattt gcccgtcgat
     3661 atcctcataa aatatcctgc atcatctttg gattttgcat aaccattact atcgtaagta
     3721 atgggtgtgc cggcggaatt aaacacgccg aagcgaagac cattagcgct gtcaatgact
     3781 ccatccagcc gaaggtctat acttaaccga atataatctc ccggctttac aagctggatt
     3841 gtatttatat tggcagcacc ccaatacgag caataggttg caggatttgt aagaaaaagc
     3901 gcgtagccgg aattcagagt accggcgccg gtatcctttt ttaccgtcag cggggaagct
     3961 gaatctaaat cgctgaatcc ataccagccg ctgcgattgc cgtcttcaaa atcatcactg
     4021 aaaacaagta cagcccctgc ggaaaaattg agtgaaacga tcattaggaa tagaactaat
     4081 cttcgcattt tacctatctc caagctgtgt tgttgttgaa agcagaatac agttcagcaa
     4141 tttttaagaa ccaacctgca ttatctttca gcggccgaat ggccagtatc cagccgccga
     4201 aagcagtcat cttcaagcag cagttgtata agctgatgtt tttctgctga cttccgcttg
     4261 ttggtgattg atccaaaaag gggccggccc aaaatcggtc agtgatcttg cggccggccc
     4321 cttaaagttg atggaattta cgcttttttg aactttccta aaaacaatcc gcccaggccc
     4381 agcaatatca atgtcgcagg ctcagggatt gccggtgtgt gcagcgcggt caattcatac
     4441 gttacactgt ccaagcgggc taaaaaccgg gtattgccaa gatagttctc agcgtcttgc
     4501 agatcggcag ccagggcagc ttgcgcttcc ggcgtatcag acgaataacc gtaagctccc
     4561 cagagtgcat tgcccatcga acccatgaca ccatcgatga cgctgtcacc attgccatca
     4621 tagtttatcc aggagcccgg agtgacccat ttgccccagc cgtattcctc ataaggattg
     4681 taccaaacac cgtcgtactc atactcgata ctcacctgaa atctgccgct tagcgaagat
     4741 atagtttcgc cggggatata gtagaaggca tgcaatctgt ttcccagatc gccgaaactg
     4801 taaaccatat aatcgtctac cgaatagttc agagttccca cgttgccgga atcgttacta
     4861 tttgcctggt tgacgaacgt gccgttataa atggcggctt cggcggcggc tcgaaacgcc
     4921 ggccagaggg gagaaccgct tgcgttggga gcggtatcga gatacaattg aaccagattc
     4981 gcattgactg ccgttactgc cataaccgaa attgcgatac acattaaacg tttcattttt
     5041 ctttcctccg aaaatttaag tatttgttgt tttagattct tgtttttaca tttcacattt
     5101 tacattactt ttggtcactg actgatttac attttgttac tggctgatta acgcttcata
     5161 gcactttcct ccgaatccat tggttatttc ccctgttaat cacagcttat caatcaccgg
     5221 ctgatttcgg cacttcatga tcactgacct gattcattct gtacctggcg attttggtac
     5281 atttcccgcc gtcggcttaa ccgccagcag cttaaagcgn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnc tggcgatttt ggtacatttc ccgccgtcgg cttaaccgcc
     5461 agcagcttaa agcgatttta tcctttctct ggccgcagaa gttatttgtc cagctgcccg
     5521 cttccagcaa tccgctttca gcttttggct ttacagtggg gccgacccaa aatcggccag
     5581 tgatcttgac gccggccctg ctttgcgatt tataaagcta ccctattttt accttcataa
     5641 aaaagagccc gcccagtccc agcagcagca tcgttgccgg ttcgggtgtc tgatgaatga
     5701 cctgtgcgtc aatgatattg ccgttattat cgaccaaagc gatggttaca tcacccggac
     5761 catcacagtg aaacagcgtg tcttgaatca gccagccgcc tgccccctgc ggtgttatat
     5821 tggaaataga cacaaaaggg gactgcattc ccacctgttc ggctccgcca gcggtcagct
     5881 cagcgctgac gccttgggat aaaacggctt ggctgatatc cagactgccg ctggcagccc
     5941 ctacagcaat gcccagcgcc atgctgcctg ttccgcctgt accgtcgaca tcgataatgc
     6001 cgatcttgat gacatcgctg gggcgaatgg ttatcttgct gtcggggtaa tctgcccaca
     6061 gaccgttgcc gaggttgaca tagattttca atccggcttc ggccggaaca actgccgctg
     6121 cggccagcgc agccactaaa aatactgtta ctcggttcat tttctttcct cccacaaatg
     6181 gtgttggtat cgtttattca ctgcctttgg caaactgctg tccaagaaga atggggccgg
     6241 cccaaaatcg gccagtgatc ttgacgccgg ccccacaaaa tacaggaatg ctttcctcct
     6301 ccgaaagccg catcctgagt taaagagctt ctgcaaagtc tttttgggct gccgccggca
     6361 tccagctgcc ggcgatccta tgttcagcat caccgtgcgg tgatgattta attctggctg
     6421 ggcacaacag cccgatcact ggccaaagag atcactaacc gttttaccca gcttggagaa
     6481 taggagagat gaaaagaata ttttcagctc tttgcttttt gtattcactt gtcaaaaaga
     6541 accgcactat agcatcgcgg cattggccgc ggttattgtc ctgccgggtn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cagttgttaa aagaaccgca ctatagcatc
     6721 gcggcattgg ccgcggttat tgtcctgccg ggttcaaccg cacgatcacc ggccgccaaa
     6781 cccagcggtg caaggggtga tgaggccctt gcgagaaagg agagagatga agaagttttg
     6841 ttcagcttct tttgctattc agttgttaaa agaaccgcac tatagcatcg cggcattggc
     6901 cgcggttatt gtcgtgctgg gccaaactgc ctgatcactg accgttttgc ccagcgctgt
     6961 gtgagaggag aatggaagat gaagcttgtc aaccttcgta tcctcaggta ttcacttttt
     7021 aattactgca tctggcctgc tgccactgtt accgctgcaa tgtcttcgat atgcagaatc
     7081 tgacagaccg tacttcgaaa gtaatcatca tcgatgtcgt cgcttaagat gacgtttacg
     7141 cccagactgc gtgccacaac ttcctgttcc atattgcccg gctcaataaa ggccactgtc
     7201 ggaatctttg ctcgtgcggc cgtgattttc tccagaagca tcccatcggg catatccacc
     7261 acattccacc ggctgaccac ggtatcaatc tgctcttccc gcaagcatcg aatagcttct
     7321 ttgccggtgt ccattaccag cagccgcagg ggcaattcct gaagtgcttg ggctttgtcc
     7381 tgtgctgcga caattaaaat ggtcgaataa atctggcaca cgttgttgtc ctgttttcac
     7441 ttgtaagcgt tattttcaaa tctgcgtttc tgtatttcct ataacaacgg ctgtgccaga
     7501 cctataaaaa aaccggaaag aattgtataa cctctttgaa aaaaagaagt tatggaatgg
     7561 ccggacaaaa atcaatcgcc ttgaaaacaa atgcccctgt tactgtttag taacagaccg
     7621 gcggaggcag aggattgaag tttttaacaa acagagactt aggcgtcagt tgcagttttg
     7681 taacaacatt acaatttggt aacaggctca gaacttatgg gccagccagt gatcggcggc
     7741 cagcacaata tctgccagcc ccactgtgcc gtctttgttc aggtcagctc cgccgcacca
     7801 gccggggcag gtacaggatg tttggagcca gcgggcagct atccaggcta agtcggcgat
     7861 atccacgtcc tcgtccggct caaaatctcc ggcgatgggg aagacctcaa aggctcctat
     7921 atctgcccgg ccgtagcgaa tccgcggctg accgtccatg tcggcagaac cgatgtctgc
     7981 aaggttcggg ttggccgcat tgatgcagat tgaatcgtgt ctgagccgat agtcgccggc
     8041 tgtgcgattg acaaagcccg ggtcctgcat aatgtcgccg gcgcctgccg gcaggtttac
     8101 ataattgctg ccggtgttgt acatgcaatt cgagaaaagc gtcggcagat agggccttga
     8161 cgaatccagc cagatgcccg atgagttaaa ggccatcacg ttgttggcaa tcagtacgtt
     8221 ttcggacagc agttgcagcg caatcgcgcc gccgcgctca ccataaaaca gatgcgtggc
     8281 ggtattgccc gaaaaggtgt tgttcaggat agacagcgta ccggttgggt tttgcgggtt
     8341 gatgcagtaa ataccgccgc cgctgccgcc ggccatgctg gagacgtcat aagcgatatt
     8401 atcgataaag aggttgttgc gaatgattcc ccaatcgctg ctggccaatg taatcgccgc
     8461 cccaacagat gcctgattgt tcttaaagtg gttgttttca atcacaaacg ccggacataa
     8521 gttcaaatcc actgctcctt ccggcgaacc catgtaaacc ggcggcagcg catacatctg
     8581 atttccgatg atggtattgc ctttaatcag cggcgtcgaa acatagccgt aaatggctgc
     8641 cccgcgtcca cagtggttct gtgtaatcag gttgttttca atccacggcc cggacaggtt
     8701 gcagtaaatc ccgccgcccc aactgtcaaa agtcagagtt tcgttccacc gaatgacatt
     8761 atcgcgaata atcggatagc ccataaaaca ggcgatgccg ccgccgcggg caaagtatgc
     8821 cgcatagggc gtccctaatg aattgtactg aatcgtatta ttctcgataa gcggcgaggc
     8881 gacccgcata taaatgccgc cgccgatgcc ctgcactcca accaccgcaa agggattccc
     8941 atctgtataa acgccgccgt tctgaaccgt ccagccgtct aaggcgcaga ccaagtagcc
     9001 ggcgttgaga attgttacaa tcgtcggcgt tcccgtccca tccaaaatgg tcgggttggc
     9061 gacgacattt cgctgcgacc gctccgtttc attgcccgca aaaccgccgt aaagatacac
     9121 atacgccgac agattgatac gttccgggta tgttccggcg gcggcccaaa tttcaccccc
     9181 gctgttttgc accgcggcaa gagcccgccc aatcgtcttt tttgcggttg cccagctttg
     9241 tccgtcagcc tcatcattgc cgtccggttt gacgcgaatg atagggtgat cggtatccca
     9301 cagcgtaccg tctgattcat cggcgccaat atccacgctg ccgccttgga tgcggtcctg
     9361 cccgtcgata tcgtctgtcc agtttgcatc gatcaccgcc gataatcccg catcgatgca
     9421 gggagagccg ggctgaagat gcagcttgcc gaaccggtaa tcggccagca ggggggcagc
     9481 gctgatattg ccgtcgatgc cggtctgact ggacagaccc acataatcca gtgtgtcgtt
     9541 ttgtgtctgg ttgccgtaca cgcaattagc ccgaatgtcg cagccggtta agccgaatac
     9601 atattgatgc aaaccgatgg tgttataggc aatcagattg ttgacgatag tgggggtggt
     9661 gttggaccaa taaatgccgg ctccctgatt agagacaata gtattgttga tgatttcggc
     9721 cacacccaaa tcggtgcgga tggaattgat aacgcagatg ccgccgccct tgaaggcggc
     9781 gttggcggcg atgacattgt tgtagatttc cggagcggcc accgaatcgg ggataggcca
     9841 gccgtccagg tcggtcgaag aggcgaaaat ggccccgccg ccgtcggttt cggtgatgta
     9901 gtcggtataa taattgaccg gcgtgctggc cgagttggcg aagatatagt tgtaggcaat
     9961 cagcggctgg ctgctgcgcc agcaggcgat ggccccgccg ttgcgcgtgg cctcgttgcg
    10021 aagaaatgtg ttgccgatga taaacgggga tgaaccgata acggcgatgc cgcccccgtc
    10081 tccttcatca ttgccggagc gattttcgac gatgacattg ctgccgatgt acgggacttc
    10141 aacagggggg acgaccttaa atccccaaac ggagatgccg gcaccggcgc catcggtttt
    10201 gttatagata atcaggttgt tgacaatggt tggggccgag ccaatgattt taatcccgcc
    10261 gccgtgaata ccgtagcccc ggatgaccgc acacccatcc agccgggtgg tggagtcggc
    10321 gctgttgtca atcgttactg tgatgcccgt gtactgggca tcaataatgc tcatgtatgt
    10381 gttataatct cgctgggaca agtcggtttc tgtcccggca aagcccccat acagagctac
    10441 accatctgga atatgaatcc gttcctgata aacgccttct ttaatccaga tttggtcgtt
    10501 gtagtcggcg gcattaatgg cggctgtaat ggtcaaaaaa ccgttatgcc aactggtccc
    10561 gtcctgactg ccgccggcat tgctattgac atagatgatt ttgccgtaaa gatgtcctga
    10621 aagagataaa aacagcacac ttactgtgaa aatataacaa tgaccccact gtctcatact
    10681 tctgcctcct tatattatgc aggccgaacc cccattattt gcatgcaatt ataatattat
    10741 cggccttatt aaacaattag caaaaagcag gaaatatact cactctgatt tgtcgcctat
    10801 aagattcggc ttttataggg attttttatg gttcagcgca caaaaaaagg ccctgctttg
    10861 tgagcagggc ctttttaggg tcacagttgg tgcacttaaa agcagtcgaa acgtctgact
    10921 tcacccgccg ttatgagcac tgctgctccg gcggggtcta agttatccct tagaaaggag
    10981 gtgatccagc cgcaggttcc cctacggcta ccttgttacg acttagtgcc agtcaccgag
    11041 cttaccgtcg gcaggcgtct cctttcggtt aacaacccga cttcgggtac tcccggctcc
    11101 catcacttga cgggcggtgt gtacaaggct caggaacaca ttcaccgcgt catcgctgat
    11161 acgcgattac tagcgattcc gccttcatga aggcgagttg ccagccttca atctgaactg
    11221 gggccgtttt tttgcgattt gctccacctt gcggtcttgc gtcgctttgt aacggccatt
    11281 gtagcacgtg tgaggccctg gacataaagg ccatgaggac ttgacgtcat ccccgccttc
    11341 ctccggtttg acaccggcag tcccgttaga gtccccgcca tgacgcgctg gcaactaacg
    11401 gtaagggttt cgctcgttaa aggacttaac ccgacatctc acgacacgag ctgacgaca
//
LOCUS       DAOTBO010000089        10977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5092_length_10977_cov_5.522432, whole genome shotgun sequence.
ACCESSION   DAOTBO010000089 DAOTBO010000000
VERSION     DAOTBO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_10977_cov_5.522432"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLE88_09880"
     CDS             <1..408
                     /locus_tag="PLE88_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM76812.1"
                     /translation="VGRFARTLGSLTASGVTILEALHVVRGTLGNEVLAVQIDNVAAS
                     VKTGSNLADPLEKSGLFPALLVQIVAMGEQTGKLDELLLNAANTFDEEADAVMKRFLA
                     VFPAVMILVLAVLIFFIIMATLLPIMTMDMSVM"
     gene            514..939
                     /gene="gspG"
                     /locus_tag="PLE88_09885"
     CDS             514..939
                     /gene="gspG"
                     /locus_tag="PLE88_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOM76813.1"
                     /translation="MGSRQSRKGFTIIELVAVAMIIALLAVFVVPRAFKGLGKAKHDI
                     ARGKMGILEGALSRFQYDCGRFPTDAEGLEALLTAPPDLEEKWGGPYCKRSDLLDPWN
                     RPFEYIAEGVVNVGSFDLICLGADGVEGGEGDNADILND"
     gene            932..1501
                     /locus_tag="PLE88_09890"
     CDS             932..1501
                     /locus_tag="PLE88_09890"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOM76814.1"
                     /translation="MTDSARNRKAWRRTGGFTLIELMAVALLTALLSSLGLISAVKTF
                     KHWSIEENAERFFLTARYARVYAIENQQPCFLVIDAAQKQFFLSAMEDVEISAETPML
                     IQNLWAKRENLDSHVQFETVRKDIMDGTAAQSQILFRPDGTAENAGIQIGDGKTHYSI
                     VISAAGRTRVLRGPLQEIEPDQVDLDLAE"
     gene            1498..1956
                     /locus_tag="PLE88_09895"
     CDS             1498..1956
                     /locus_tag="PLE88_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76815.1"
                     /translation="MKKKGVSLLETILAAAILSGAVVTVCGIGGRSLRSMRLNQEYEK
                     AWDYLNRQLVLIDQAGPDVLLQSGQLSGQFASADGRQWKWTAAVEKSSITNLYDVAVR
                     MEWPSGSRICQVQCHTRLCGRGAALEMPEENTAGQSSSPSGRQVPQEASQ"
     gene            1953..2657
                     /locus_tag="PLE88_09900"
     CDS             1953..2657
                     /locus_tag="PLE88_09900"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76816.1"
                     /translation="MSRRCCNRGFTLVEVLAASVITAFIILVAVNGLVSAMSARSRLD
                     EAARTADELRYAADSIQQDLRRVYRDGTELLFEGVLEQTPSGEFPRLRFRAVRYEKAR
                     AAEPEGDLYEVEYFLMPNQDNLSLARRVCPIVGIETLPAETAGGVLTKLNEAITYFGI
                     RYFDGSAWTAQWPQELNRLPMLVEVSLVMRVQESEKKERIYARQVLVNFPHYAGQSEI
                     QTEEIQWEDPEQLMAQ"
     gene            2660..3964
                     /locus_tag="PLE88_09905"
     CDS             2660..3964
                     /locus_tag="PLE88_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOM76817.1"
                     /translation="MQKKPFRQKGWGLALVVVLWMVMLMSVLVTLAARGSLLDTKISL
                     VEVEKQRCRWAARGGVETAIALLDEDDRTSDSMDDLWSNNPYELEQLKIGQLDVRVQV
                     TDAASKLNLNTVSRNQLLWIPDMTEEIADSILDWRDSNDQPRAQGAESGYYLNLPFGY
                     QARNSRFHTLRELLRVKGVTPALLEGEDSQTLSPENQGWKHYFTPVSLEWNRDTQGQP
                     KVNLNQADQTELTQTLSLTEQQAQWIMQNRPFRRWAELLGGRSVSGQNTPTPARAQSG
                     AVSQTPRQQQGQATQRTGENPPENTGGPLDWETLMNIIDKSTLTSNSYLSGRINVNTA
                     SITVLTALLAGNRQLAENIAVWRQTSVSGFSSVGDLEQVEGMTREILTDLLDFITVRS
                     SVYEIRSTAASAATNLTYTIEVLVNRDADGGQVLYWHEGVQL"
     gene            3961..5340
                     /locus_tag="PLE88_09910"
     CDS             3961..5340
                     /locus_tag="PLE88_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76818.1"
                     /translation="MSTADAYFRTAAGITICGAEGTAFVVIMQQQAGGIKMLDKQQMS
                     LSEGMERLCRRVRQMADGQDLRLIIGMDCAQVRFLQVTLPAVEPEAVSELLAMQIESR
                     LPIPSSQVSWVWSSQSRLDGLHCSIAAVKKDFLAKSAPSLDGDMICTLEAVGLAALWR
                     RVFGDQGGHQMLLWKRPKDFLLTAVEGGRLMHACVIDAESADGQSLPDYCVQDLLDVL
                     DSLGSSREDPLLLIAPQDALTGGIKNQLEQAGRKTVICPASELKQSLPELTLIDDPFL
                     PAAGLAMAGLMPDSIDYDFVQIASQPSRLARSAAASVQLRRNAAVTIGLLMLCLALFF
                     WMMKKETAMMQKTLSISYDKLTAEEVLQHQAFREAVARARPDLLDVFEKIQNSRQGVT
                     LDSFEFEKGKPIRITATGPGYEAVYAFQRQMESQSGISQVRLLDPRLNERDKTVQFTM
                     TFYYKHFSQ"
     gene            5348..5887
                     /locus_tag="PLE88_09915"
     CDS             5348..5887
                     /locus_tag="PLE88_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76819.1"
                     /translation="MMKPLSAREKRAVGLVVLTAVLIAVWRVSVPAFEYYQRLTGQRH
                     ALRQKIDTLFLLAKPEQQLRLKTLSEQVPVLEMPQPAAQQGILLRDELTRQLQRCGIQ
                     AKTLQLRESSGALRRDGFRVLLLDCQGRCSTQSLMQLIEQLKQNPYYVAVEKLSMKVD
                     PKERSAVTFQLVVSTYAKG"
     gene            5898..6551
                     /locus_tag="PLE88_09920"
     CDS             5898..6551
                     /locus_tag="PLE88_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76820.1"
                     /translation="MNRIYELAFKHQQGCFYGLWAAMLIWLMVQTGGFAMRWWLQSGR
                     IEESVRQWQQVMHSAAPSTKAGSRAGTLASQGALFAPPPGKPPLPKCMAILGDAVLIN
                     DQWYKVGSQVQGVKILEILPQAVRVEQDGQEHKLVPFESQPEQEQRDRAARSERGDAR
                     SGTAASVRAENRPSPSNVPSAPGTMTAEQRRLYERYQNASPQERARLRDEFRRNRGQ"
     gene            6555..9617
                     /locus_tag="PLE88_09925"
     CDS             6555..9617
                     /locus_tag="PLE88_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOM76821.1"
                     /translation="MKTPNPNRVKTIIACCAIVIAGFVIAAVSAEDTSQQRAGRPERW
                     QRPQAYRADQPSADPNAPAASAEPNAPASSDKPASPDSAGKDPNAALEAVNLNNVEMK
                     NIIQMIAEWTGKPVIPTSDEILQAKLTIYSPKKLPRAEALSLISLALQSKGVVLEILE
                     DKIFMRPLASVRLGAVPTIGPDEPLARFEDKSQIVEKWFQLKTYSPSKLIQIVAPLTA
                     EYGYTAADEGTSRIVVIDTVENLMRIERLIQQLDVPESAREVEKIFELQYGDPLEIVQ
                     VLELIFSQRDRRFGGGGPVQEARPAGSRSRELPTALSVVITGTTVPIRLIPMAKQKWI
                     LARASREDMTRLEEWIRKLDIAETEDLKQTVVQVRYADVREVVRMVQSTLREMPGTEL
                     QANLVVEALPQNNQIVIYGSESNRKVVEKLIAQIDLPAEDVFIEKTFQLKHADPDKIK
                     ENITNLYDTQAGSISSYSYRSGYSSSRYRTVSPEDVVRVVSYPMIKQITVIASEKNLT
                     KISQQIAEWDKPIDVETDQYRILSLQNSDPVQMADLLKKLFSEESDSSGRNLIRLLLG
                     GDDAESRRKIVGSLYGMLTFEPVPATKKLIIISQIPEAYAVIERLVEKLDGQESAEVP
                     RVITLKYADAEDLCDQLNAILNEAGTAATIQRSRRGLSAYSADSAQARGAAAGGNESA
                     STITPWWTRQRPNANELPPSNLIGRVRFVPVHRSKAILALAPAEYMDDITAMIEELDQ
                     PGMQVMVKVVIMDISLSEATSLGVQLASDPSAFGTLGVNALAALNQFTAGVSRGSFAY
                     TTSADISLLVDLLIKHANGRVLNQPTLWTKDNEEAVFVKGQKIAFIDTEQSDSSNLAA
                     TKRTFSYEDVGVTLRIRPNITPEKAVDMTINLNISQVLQELINTQVARTNLDTTTHLI
                     VNDGQSIMLGGILEQNNNTTQQKVPLLGDLPLVGGLFKHTKSELSNSELLIFITPYVI
                     DNRILNSIPVEGSAGEYLQESRLRMDNALDDLAEVVRRRWGQSADPNE"
     gene            complement(9625..>10977)
                     /locus_tag="PLE88_09930"
     CDS             complement(9625..>10977)
                     /locus_tag="PLE88_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76822.1"
                     /translation="AERADNARQINTLQEQLDGAVRQIQIQDSQIQELNRQYEQLAAT
                     HRDLQVQYEQQTRTLDAERADNARQINTLQEQLDEKTGLTESLQAQIDILKVTEEAYH
                     GTLEQMQLIQTKQEELQGRLEAKISELERAHNQLEQSRLERHKLEEELNHQKLCCQQL
                     NQQKDNLASQIETAAAASEQIQKQYRQILDELEFRKRQFKSELDTRDQQIRSLQERLQ
                     EALHQIQEFESMLELSRTEASQLRQTADKTTQQIIHLQSRIAQQNMEYAALQDQLASL
                     QRCPIELSDNPSDFENAQIRIQLLSQDLTRETQAHAAARQELETCKTHLNRLENQLQE
                     QNQLIQSLQQEKQDSAVQLCQMQQELESLQKASANVLGLERRYKEAVQRVENLTAQVK
                     EQTESLKKAHQTINFLRMQKTPSSVRIPGVDTPARPLVTPPCGPKTVQSRQPNTSGNG
                     "
BASE COUNT         2520 a         2651 c         3233 g         2573 t
ORIGIN      
        1 gtagggcgtt ttgcccgtac ccttggttcg ctgacggcca gcggggtaac cattctggag
       61 gcactgcatg ttgtccgggg gacacttggc aatgaagtgc tggcggtgca gatagataat
      121 gttgccgcca gcgtcaagac gggctccaat ctggcagacc cgctggaaaa gtcaggactg
      181 tttccggcgc tgctggtgca gattgtagcg atgggcgagc aaaccggaaa gctcgatgag
      241 ctcctgctta atgcggccaa tacatttgat gaagaagccg acgcagtgat gaagcggttt
      301 ttggcagttt ttccggcggt gatgattctg gtgctggctg tgctgatctt ttttatcatt
      361 atggctacgc tgctgccaat tatgacaatg gatatgagcg ttatgtaagg gtgttaatgc
      421 ccccaacggc tgtgattgga aatacggttt ttgaggattt agatagacgt gtttaaggcg
      481 agtgaagatg taaaacagga taggatgcgc tatatgggca gtagacaaag cagaaaggga
      541 tttacaatta ttgagctggt tgcggtagca atgattattg cactgctggc agtgtttgta
      601 gtgccccggg catttaaggg cttgggcaag gccaaacacg acatcgcacg cggcaagatg
      661 ggtattcttg aaggggcact gtctcggttt caatatgact gcggccgctt ccccacagat
      721 gccgaagggc tggaagcgct gcttacagca ccgccggatc ttgaagaaaa gtggggtgga
      781 ccctactgca agcggagcga tttgctggat ccctggaacc gtccgtttga atatatagcg
      841 gaaggggttg tgaatgtcgg cagttttgac ctgatttgtc tgggagcaga cggcgtcgaa
      901 ggcggtgaag gcgataatgc ggatattctt aatgactgat tctgcccgga accgtaaggc
      961 ttggagacga acaggcggtt tcactctgat agaattgatg gccgttgcgc tgctgacggc
     1021 gctgctgtct tcgctggggc ttatttcggc cgttaagact tttaagcact ggtctatcga
     1081 ggaaaacgcc gaacgttttt ttcttacggc tcgttatgcc cgggtgtatg caattgagaa
     1141 tcagcagccc tgctttcttg tgattgatgc tgcacagaag cagttttttt tgtcggcgat
     1201 ggaagatgtg gagatatccg ctgaaactcc aatgctgata cagaatctct gggcaaagcg
     1261 ggaaaatctg gactcccatg tccaatttga aaccgttcgc aaggatatca tggacggcac
     1321 agcggctcaa agccagatac tgtttaggcc ggatggaaca gctgaaaacg ccggcattca
     1381 aatcggcgat ggaaagaccc attattcgat tgtaatcagt gctgcaggcc gcacacgcgt
     1441 attgcgcggc cctctgcagg aaatagagcc ggatcaagtg gatctggatt tggcagaatg
     1501 aagaaaaaag gtgtcagttt actggaaaca attctggcgg cggcaatttt gtccggtgcg
     1561 gttgtgacag tctgcggtat aggaggccgg tccctgcgtt ccatgcggtt gaatcaggaa
     1621 tatgaaaagg cgtgggacta tctgaatcgc cagctggtgc tgattgatca ggcaggaccg
     1681 gatgttctgc tgcaaagcgg ccagctgagc ggacagtttg ccagtgctga cggtcgccag
     1741 tggaaatgga ctgctgccgt agaaaaatcg agcatcacaa acttatatga tgtagcggtg
     1801 cgaatggaat ggccttccgg aagccggatt tgtcaggtgc agtgccatac gcggctgtgc
     1861 gggcgcggcg cagctttaga gatgccggag gaaaacaccg ctggacagtc gtcctcccca
     1921 tccggccgtc aggtgccgca ggaggcatca caatgagccg ccgctgctgc aatcgcggtt
     1981 ttacactggt ggaggttttg gccgcttcgg tgattacggc atttattata ctggtggcgg
     2041 ttaatggtct tgtcagtgct atgtctgccc gaagccggct ggacgaggcg gctcggacag
     2101 cggatgaact gcgctatgca gcagacagca tccagcagga tttgcggcgg gtttatcggg
     2161 atgggacgga actgctgttt gaaggagtat tggaacagac gccttcgggc gaatttcccc
     2221 ggcttcgctt ccgggctgtg cggtatgaaa aggcccgtgc cgctgagccg gaaggcgacc
     2281 tttacgaagt tgaatacttt ttaatgccca atcaggacaa tctgagcttg gcaagacggg
     2341 tttgcccgat agtgggcata gaaacattgc ctgcggaaac cgccggtggt gttctgacga
     2401 agctgaatga ggcgataacc tattttggga ttcgctattt tgacggttct gcctggacag
     2461 cccaatggcc gcaggaactc aaccggctgc cgatgctggt ggaagtctcg ctggtgatgc
     2521 gggtgcagga atctgagaaa aaagaaagaa tatatgcccg gcaggtactg gtgaattttc
     2581 cgcattatgc cggccagtct gaaatacaga ctgaagagat acagtgggaa gatccggagc
     2641 aattgatggc ccaatgacaa tgcagaaaaa accatttcga caaaagggct gggggctggc
     2701 actggtggtg gtgctttgga tggtgatgct gatgtctgtg ctggttacat tggctgcgcg
     2761 agggtccctg ctggatacga aaatcagttt ggtggaagta gagaagcagc ggtgccgatg
     2821 ggcggcgcgc ggcggcgtgg agaccgcaat agccctgctg gatgaagatg accgtacgag
     2881 cgactcgatg gacgatttat ggtcgaataa tccctatgaa cttgagcagt tgaagattgg
     2941 ccaactggat gtgcgggtgc aggtaacaga tgccgcttca aaactgaatt tgaatacagt
     3001 cagccgcaat cagctgctgt ggattcctga tatgaccgaa gaaattgccg acagcattct
     3061 tgactggcgc gacagcaatg accagccgcg agcccaggga gcggaaagcg gttattatct
     3121 gaatctgccg ttcggctatc aggcgagaaa ttcccgattt cacacgctgc gtgaattgct
     3181 acgggtcaag ggagttacgc cagcccttct ggaaggagaa gacagccaga cgctatcccc
     3241 tgaaaaccaa gggtggaaac actattttac gcctgtatcg ctggaatgga atagagatac
     3301 acagggtcag cccaaggtca atttgaatca ggctgaccag acggaattaa cccaaacgct
     3361 gtcgcttacg gagcagcagg cgcagtggat tatgcagaac cgtcccttcc ggcggtgggc
     3421 cgaactgctt ggcggacgtt cagtctccgg ccagaacacc ccgacaccgg cgcgggcgca
     3481 gagcggtgcg gtatctcaaa ctcctcgtca gcagcagggg caggcaaccc aaagaactgg
     3541 cgaaaatccc ccagaaaaca ccggtggacc gttggattgg gaaaccctga tgaatattat
     3601 cgacaaatcc actttgactt caaacagcta tttaagcggc cggattaacg tcaatacagc
     3661 cagcataacc gtactgacag cattgcttgc cggcaatcgt cagctggcag agaacatcgc
     3721 tgtatggaga cagacttctg tcagcggttt ttcatcggta ggggatctcg aacaagtcga
     3781 agggatgacc cgagagatac tcaccgatct tctggatttt ataacagttc ggtccagtgt
     3841 ctatgaaatc cgcagtacag ccgcatctgc ggcaacgaat ctgacatata caatagaagt
     3901 gcttgtcaac cgtgatgcag acggcgggca ggttctttac tggcatgaag gggttcagct
     3961 ttgagcacag cagacgcata ttttcgaaca gctgccggca taaccatttg cggggctgaa
     4021 ggaacagcat tcgtagtgat tatgcagcag caggccggcg gcatcaaaat gctggacaag
     4081 cagcagatgt ctctgtccga aggaatggaa cggttatgca gacgcgtccg gcagatggca
     4141 gacggtcagg acttgcggct gattatcggc atggactgtg cacaggtacg atttctgcaa
     4201 gtgacccttc ctgccgtaga gccagaggcc gtcagcgaac ttctggcaat gcagatagag
     4261 tcccgtcttc ccataccatc cagccaagtt tcgtgggtct ggagcagtca atcccgcctg
     4321 gatggtcttc attgcagcat tgctgcggta aagaaggatt ttcttgccaa atccgccccc
     4381 agtctggatg gagatatgat ctgcaccctt gaggcggttg gattagcggc gctgtggagg
     4441 agggttttcg gagatcaggg tggacaccag atgcttttgt ggaagcggcc gaaagatttt
     4501 ctgctgacag ctgtcgaggg cggtcggctg atgcatgctt gtgtgattga tgccgaatcc
     4561 gctgatggtc agagcctgcc ggactattgc gtgcaggatc ttctggatgt gctggacagt
     4621 ctcggcagca gcagagaaga cccgttgctt ttaatagccc cgcaggatgc attgaccggc
     4681 gggataaaaa accagctgga gcaggctggc cgcaaaacag tcatttgtcc ggcgtcggaa
     4741 ctgaaacagt ccctgcccga attgacgctg attgatgatc cctttctgcc ggccgcggga
     4801 ttggcgatgg caggattgat gccggattct atagattacg attttgtgca gatcgcatca
     4861 cagccgagcc gcctagcccg ttctgcagcc gcttcggtgc agctgcgaag aaatgccgct
     4921 gttacgattg gactgctgat gctgtgttta gccctctttt tctggatgat gaaaaaggaa
     4981 acagccatga tgcagaagac gctgtctatt tcttacgaca agctgacggc tgaagaggtt
     5041 ttgcagcatc aggcatttcg ggaagctgtg gcacgggccc gtccggatct gctggatgtg
     5101 tttgaaaaga ttcagaattc gcggcaaggc gtaacgctgg acagtttcga atttgaaaaa
     5161 ggaaagccga tccggattac agctaccggt ccgggatatg aagccgtgta tgcgtttcaa
     5221 aggcagatgg aaagccaaag cggcatcagt caggttcgtc tgctggatcc gcggctgaat
     5281 gaacgggata aaacagttca gtttacgatg acattttatt acaaacactt tagtcaatag
     5341 aggccgcatg atgaaaccat tgagtgcccg tgaaaaaagg gctgtagggt tggtggtttt
     5401 aacagctgtt ctgatagccg tctggagggt gtctgtgccg gcctttgagt attatcagcg
     5461 gcttacaggc cagcggcatg cactgcgtca gaaaatcgat acactgtttt tgctggccaa
     5521 gccggaacag cagctccgcc tgaaaacgct ttccgagcag gtgccggttc tggaaatgcc
     5581 gcagccggcc gcccagcagg ggattttact ccgtgatgag cttacccggc agcttcagcg
     5641 ctgcggcatt caggccaaaa cacttcagct tcgggaaagc tccggcgcct tgcggcgaga
     5701 cggctttcga gttttgctgc tggactgtca gggacgctgt tctacccagt cgctgatgca
     5761 gctgatcgag cagctcaagc agaatcccta ttatgtggct gttgaaaagc tgagcatgaa
     5821 agttgaccct aaagagcgaa gcgctgtaac atttcagctg gtagtgtcca cctatgcgaa
     5881 aggataaaac tgttaaaatg aatcggattt acgagcttgc cttcaagcat caacaagggt
     5941 gtttttacgg tctttgggca gcgatgctga tctggctgat ggttcaaacc ggcggatttg
     6001 ccatgcgctg gtggcttcag tccggccgga tagaagagtc ggtcagacaa tggcagcagg
     6061 ttatgcattc ggcagcaccg tcgacgaagg cgggttctcg tgccggcacg cttgcatcgc
     6121 agggagcgtt gtttgctcct ccgcccggca aacccccgct gcccaaatgc atggcgattt
     6181 tgggcgatgc cgtgcttatc aatgaccagt ggtataaggt tggaagtcaa gtgcaggggg
     6241 tcaagattct ggagatactg ccgcaggccg tccgcgtgga gcaggatgga caggaacata
     6301 agctggtgcc ctttgaaagt cagccggagc aagagcagcg cgacagggcg gcgcgctcgg
     6361 aacgggggga cgcccgttcc ggcacagcgg cctctgtcag ggcagagaat cgcccgtcgc
     6421 cgtcgaatgt tccttctgct ccaggcacaa tgacggcaga gcagcgccgg ctttacgaac
     6481 gttatcaaaa cgcctcccct caggagcggg ccagattgag agacgaattc aggagaaatc
     6541 gaggtcaata atttatgaaa acaccgaacc ctaacagagt caaaacaatt atcgcatgtt
     6601 gtgcaatagt gattgcaggt tttgttattg ctgcagtcag cgctgaggat acttcccagc
     6661 agcgagccgg caggccggaa cgctggcagc ggcctcaggc ctatcgtgcc gaccagccgt
     6721 ctgccgatcc caatgcgccg gcggcatctg ccgagcccaa tgcccccgct tcatcagata
     6781 agcctgcatc gccggactcc gccggcaaag accccaatgc cgcactcgaa gcggtgaatc
     6841 tgaataatgt ggaaatgaag aatattattc agatgattgc tgaatggacc ggcaaacctg
     6901 tgattccgac cagcgatgaa atcctgcagg caaaactgac catttactcg ccgaagaagc
     6961 tgccgcgtgc ggaagccctt tccctgattt cgctggcgct gcagtccaag ggggttgtgc
     7021 tggagatact ggaagacaag atttttatgc gtccgctggc ttcggtgcgg ctgggggcgg
     7081 tccctacgat tgggccggat gagccgctgg cacgctttga ggacaaaagt caaattgtag
     7141 aaaaatggtt tcagctgaaa acctacagcc cttctaagct gattcagata gtcgccccgc
     7201 tgacagccga gtacggctat acggcggcag atgaaggcac atcacgcatt gtggtgattg
     7261 atactgtgga aaatctgatg cgcatcgaac ggctgattca gcagctggat gttcccgagt
     7321 cagcacgcga ggtcgagaaa atctttgagc ttcagtacgg cgatccgctg gagattgttc
     7381 aggttctcga attaattttc agccagcgcg accgccgctt cggtggcgga ggtccagtac
     7441 aggaagcccg gcctgcaggc tctcgcagca gggagctgcc gaccgctttg tctgtggtga
     7501 ttaccggcac caccgtcccg attcggctga ttccgatggc caagcagaaa tggattctgg
     7561 cacgggcatc ccgcgaagat atgacccgtc tggaggaatg gattcgcaag ctggatatag
     7621 ccgaaacgga agatttaaag cagactgtgg tgcaggttcg ctacgcggat gtgcgcgaag
     7681 tggtgcgaat ggtccagagc acactgcggg aaatgccggg tacggagctt caggcgaatc
     7741 tggtggttga ggcgctgcct cagaataatc agattgtcat ctatggcagc gagagcaacc
     7801 ggaaagtcgt cgaaaaactg attgcccaga ttgatctgcc ggcagaggat gttttcattg
     7861 aaaaaacatt tcagctcaag catgcggatc cggataaaat caaggaaaac attaccaatt
     7921 tgtacgatac ccaggccggc agcatcagca gctattccta tcggtccggc tacagttcca
     7981 gccgctatcg gactgtcagc ccggaggatg tcgtccgcgt tgtttcctat ccgatgatta
     8041 agcagattac ggtgattgcc tccgaaaaaa atctgaccaa aatcagccag caaatcgctg
     8101 aatgggataa gcccatcgat gttgaaaccg atcagtaccg gattctgagt ctgcagaatt
     8161 ccgatccggt gcagatggcc gacctgctga agaagctttt cagtgaggaa agcgacagca
     8221 gcggacgcaa tctaattcgt ctgctgctgg gcggagatga tgcggaatcc cgccggaaaa
     8281 tagtcggctc gctttacggc atgctgacat ttgagccggt gcctgctacc aagaagctga
     8341 ttattatcag ccagataccg gaagcgtatg cggttattga acgtctggtg gaaaagctgg
     8401 acggtcagga aagtgctgaa gtgccccgcg tcatcaccct gaaatatgcc gatgcggagg
     8461 atctctgcga ccagctgaat gctattctca atgaggcagg cacggctgca accatccagc
     8521 gcagccggcg gggactcagc gcctacagtg cggattcggc acaggcgcgg ggggctgccg
     8581 ccgggggcaa tgaatcggct tccacaatta ctccttggtg gacacggcag cggcccaacg
     8641 ccaatgagct gccgccgagc aatctgatcg gacgcgtgcg gtttgtgccg gtgcaccgca
     8701 gcaaggccat tctggcgctg gcgccggctg aatacatgga cgatatcacc gcaatgattg
     8761 aagaactcga ccagccgggg atgcaggtga tggtcaaagt tgttattatg gatatcagtt
     8821 tgtcagaagc cacatcgctg ggtgtccagc tggcgtccga tccttcggct tttggaacgc
     8881 tgggagtcaa tgcactggcg gccttgaatc agtttacagc gggcgtctca cgcggctcct
     8941 ttgcctatac cacctcggcc gatatcagtt tgctggtcga tctgctgatc aaacatgcca
     9001 acgggcgtgt tctcaatcag ccgactctgt ggaccaaaga caacgaagaa gccgtatttg
     9061 tcaaggggca gaaaattgct tttatcgata cagaacaatc agacagttcc aatctggccg
     9121 ccaccaagcg caccttcagc tatgaagatg tcggcgtgac cctgcggatt cgtccgaata
     9181 ttacaccaga aaaagccgtt gatatgacga ttaatctgaa tatttcgcag gtgctgcagg
     9241 aactgatcaa tacacaggtt gcccgcacca atctggatac aacaacccat ttgattgtca
     9301 atgacgggca atcgattatg ctgggaggca ttctcgaaca gaacaacaat acgactcagc
     9361 agaaagttcc gctgctggga gatttgccgc tggtaggcgg tctgttcaaa cataccaagt
     9421 ccgaattgag caacagtgaa ttgctgattt ttattactcc ctatgtaata gacaaccgga
     9481 ttttgaacag tattcctgtt gaaggcagcg caggcgaata tctccaagaa tcgcgcctgc
     9541 ggatggataa tgccctcgat gatttggccg aagttgtccg ccggcgctgg gggcagtccg
     9601 ctgacccgaa tgaatgaacg gtttttaacc gtttccgctt gtgttcggct gccgggattg
     9661 gactgttttg gggccgcagg gcggtgtaac aagcggccgg gcgggcgtat cgacgccggg
     9721 aatgcggaca ctgctggggg ttttctgcat cctcaggaaa ttgattgtct gatgagcttt
     9781 tttaagactt tcggtttgtt ctttgacctg tgcagtaaga ttttcgaccc gctggaccgc
     9841 ttctttgtag cggcgttcga gtccgaggac gttggcacta gccttctgta aggattcaag
     9901 ttcctgctgc atctggcaca gctgtacggc actgtcctgc ttttcctgct gcagggactg
     9961 aatcagctgg ttttgctcct gcagttggtt ttcgagccgg ttcaagtggg ttttgcaggt
    10021 ttccagttcc tgccgggcag ctgcatgggc ttgggtttca cgcgtcaggt cctgactaag
    10081 cagctgaatg cgtatttggg cattttcaaa gtcgcttgga ttgtctgaca gttctatcgg
    10141 gcagcgctga agcgaggcca gttggtcctg cagagccgca tattccatat tctgctgagc
    10201 aatgcggctt tgcagatgga taatctgctg agtggtttta tctgccgtct ggcgaagctg
    10261 ggaggcctcg gttcgcgaca gctccagcat tgattcaaat tcctgaatct gatgtaatgc
    10321 ctcctgaagc cgttcttgaa gcgaacggat ttgctgatcc cgcgtatcca attcgctctt
    10381 gaactggcgt ttgcggaact caagctcgtc caaaatctgc cggtattgct tctgaatctg
    10441 ctcggaagcc gctgctgcgg tctctatctg gctggccaga ttatccttct gctggttaag
    10501 ctgctggcag cagagcttct gatggttcaa ttcctcctcg agcttatgcc gttccagccg
    10561 gctctgttcc agctggttat gtgctctttc aagttcactg atttttgctt ccagccggcc
    10621 ttgcagttct tcctgctttg tctgtatcag ctgcatctgc tcaagtgtgc cgtgataagc
    10681 ttcttcagtt actttaagaa tgtcgatctg ggcttgaagg gattcggtca gtccggtttt
    10741 ttcatccagc tgctcctgca gggtgtttat ctgccgggca ttgtcggccc gttcggcatc
    10801 gagtgtgcgg gtctgctgct cgtattggac ctgcagatcc cgatgggtag cagcaagctg
    10861 ctcatattgc cgattcagtt cctgaatttg gctgtcttgt atctgtattt gtcggacggc
    10921 tccatccagc tgctcctgca gggtgtttat ctgccgggca ttgtcggccc gttcggc
//
LOCUS       DAOTBO010000090        10891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5144_length_10891_cov_5.490772, whole genome shotgun sequence.
ACCESSION   DAOTBO010000090 DAOTBO010000000
VERSION     DAOTBO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_10891_cov_5.490772"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..2354
                     /locus_tag="PLE88_09935"
     CDS             252..2354
                     /locus_tag="PLE88_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76823.1"
                     /translation="MKQVLMFLLALAVSVPVSAVITHRYDFEADANDLVGGLKTVLEN
                     GAVVQNGALVLGGGSPGARAVLDGPGIAINTYTSVSIEAWFMDNGLSGWTRVFDFGDT
                     SGGDGGYCLFYTPSGPGRSRLAISTNGFPSWSTGEQLVDGPVLTSATKYHIVCVYEAG
                     GGSGGLSAMRIYHNGVLAASNTAVSMPLSGLHNNFAYLGSAVYPGDPELNGSIYEFRI
                     YDKALNPGEVRFTNHFGENNAYPLVIRSHNPIYGQTLVPVTPTLSWTPEANVDVLSYN
                     LYLGTDPNIMDPNRSDVSGISTLVVTNLASPSYTVPSAQKLLNNKVYYWRVDTVATDL
                     TTYQGAGMTFTSIPAFPAFTVNPVSAYVFEGQTAELTATCQSLTPLTDAVRWYKVGTP
                     DVEVTAGGDVSIQTVTVGDLTTTTLSIANVDAAREASYYVKATNAGGSAVSSSAKIVL
                     KKLIAYWPFENNLTDASGTGYHGTAVGNLTYAAGKVGQALDLSGATDTYVDLPDGFAD
                     FVGGLTFNVWAYPRSAANWARFLEFGNGANADNIYFARNGTSATLQYTIYKGSTSSGT
                     VSRTNTIALNAWQMFTITMDASGSCRLYKDGLQIATGTVQIPNVVTRINNYIGKSNWA
                     ADAYYNGLLDEIRIYNYALTPDEVADRYVADTGAPYCRTRPTYDLNNDCLFNLADFAI
                     FAAQWLDCGLYPASDCPN"
     gene            2421..4637
                     /locus_tag="PLE88_09940"
     CDS             2421..4637
                     /locus_tag="PLE88_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM76824.1"
                     /translation="MKKLNSAVVIALMLSLSIGASAATLLWYDFEDGTPETVMNPGTA
                     PAIASRDLSGNGYHMWGWASGTANNSPSFSALGDTPTGYGTSARYPVASKDGYTAGAD
                     AINTWSPLVWTIEVSAKLDSLSGWQTVIGRNGSSLGVAEADFYLQKNDIDDRWRINFG
                     TVGGERFILDSDFVVEAGKWYHIAVVSDGTTVKMYVDKIDGNGYQLVGSRAFSGTNNA
                     LAQGNFVWTFGRGWYNGGNVDFVIGNIDDVRFSDAALTPDQFLHTMYDLGNPSPGHRS
                     VNAQRSPTLSWQFINAGVTADSYQIYGPTTDPNLVDPNQPRTPDYTTTAQSQAIAGPL
                     AYTTKYYWRVDAVVGTTVYTGPIWNFTTVPPSPVFSVNPGRVGAFPGESAVLTATCSS
                     TLPLTRTIQWFKVGTPDVEITTADPDVSIQQTVNGELTTSTLTISNISLAHEAQYYAR
                     ATNSGGSVNSSNGAIVVKRLLAWYQFEGNTNDSSGNNMHGTVMTPSGAAATIAYVPSV
                     NEAMGQAVSFTGTGISNPTNTVDPYVDLPDGFSDFSQGLTFGAWVYPTAAANWARIVE
                     FGNGTNGANNSIYFTRVGTGTTVRLENDNTNPSNNTSITTPSGGITLNEWQFLVVTVN
                     KNATNNATIYRNGRPLVTGTINLPNIVTRTNCFIGRSEWYGDSLYQGRMDDVRIYNYG
                     LTADEIAQMYYDIVGTFCLSKPVYDYDNNCIEDLGDLAIMAATWLDCGLYPATACP"
     gene            4761..9287
                     /locus_tag="PLE88_09945"
     CDS             4761..9287
                     /locus_tag="PLE88_09945"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM76825.1"
                     /translation="MKSGSYFGIVLLVAAVLFVQPASAATIAHWNFEDGTAGLPFYNM
                     PRRGSVDIVNGYVMRGYNTTYGPSFTAETPDGSGLGAYCNGSQDGYTTNSVINGWSPQ
                     AWTIEISARLDDISGWETLIGRDGASHGEPESDFYLQKNNVNGAFRINYESVGGQRWI
                     LDSNFTVQAGRWYHLAVTSDGAELTMYCDKLDGSGYQVVGSLDISSQTPAQNALAASG
                     ANWTFGRGWYNNSFTDHITGYFDNVRFSDTVLAPNEFLGYNPILLNESDGKTLLFKQD
                     LVYTDDYSIVLKEAPAGDVTVTITPPAGLTVGNGSGQSLTITFTSADWNQPRTITVGV
                     ADAGALLADIEYIGHSVQSTDPKFNGVSIRDVAVYIEEDACGVWGYLETDYDFNCKVD
                     LEDFAVLAGLWLITDAPLNLTDFATDWLMNTWTYNETTYARSIQRSDQPFFVNTANIA
                     NTIDEKIYGHFLEHIYHSVNGGLWGELVWNRSFEMSDSGGGIWSIEDNQLIQSSLSTD
                     VRLPFGDTGWSDYELTLEARKDGGSEGFLIPFRYADGDNFYWLNLGGWGNVRHAVEKE
                     INGSRSTVSGTTVNGSINTGQWYSIRIRCQGNTYSIYLDNVLIISFTDSSNPHLTGQV
                     GVGTWATQARFRNIQVAQIPDSAILFSGLPDLPGTAFGAAFWSVFGTAQATMTTDALN
                     DSYAVQIIGDGSAAGLMQNNFKFIPQKYNGSLWMKGSLPAGLKVELLDNTTVLGQAIL
                     PAPSADWAEYPFQIDSAGSTNQGMLRITLLGAGTVKIDQISLMGQDAASVGGYRPDLL
                     SAVEELRPPIIRWPGGCYASAYFWKDGIGPQHQRRKYPISLWDDQDTNSYGTDEFLRM
                     CRRIGAEPLICVNTGLLNGTCGVAIPYKLTEEQYLQDVLDWMEYCNGPVDTPWGAVRA
                     ANGHPEPYNVTYWEIDNETWSTSWGGGITNYIAKVQLFAPAMRIKAEQLGVPVKLIAC
                     GSGSYDQSWNRTLIDNCAVLIDYISTHHYESPDNYKQGPRSYEAFIKELAAYIAASAN
                     PDLKIYMSEWNAQSTDWRTGLYAGGLLNAFERTGDVFEIGGPALFLRHTSASGWDNAF
                     INFDHTGWFAAPNYIVMKLWHDHYAPLCVQTNGTDTNLNVVSALSADEKTLYIRIVNP
                     DADSKSVAFEIDPSFTAESAYMHYVAPGSLYARNTLANPHAVRVEAKVVGLDDQTLRF
                     KMPGYAAAVVTVRTSKPHKTAFLYSSFRNNGEDGLHLAYSQDGLVFTALKGDTSFLAP
                     QVGSRLMRDPSICQGPDGMFHLVWTTGWWDKGIGIAHSPDLINWSEQIFLPVMAHEPN
                     AKNCWAPEIFYDEATDRFLIFWSTTIDGAFPETYNPSDDNNHRIYYVSTQDFVTYSNT
                     ALFYDPGFNCIDAFIAKHGAGYVMFLKNETKVPIAQKNIRMAFSDSAAGPWGPASESI
                     SPAWVEGPSAVKAGGQWYLYYDAYTRGRMEATASLDLKAWTDITSQLSFPAGTRHGTV
                     FRVAQDIVDALKSL"
     gene            complement(9351..10637)
                     /locus_tag="PLE88_09950"
     CDS             complement(9351..10637)
                     /locus_tag="PLE88_09950"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOM76826.1"
                     /translation="MKKLNLQSVRIQDIISKEQFGRIAAGFLRNFKLPVETTDINGQE
                     IRSLCSSNCHPAFCQRIRLSKTGLKRCREDRVRSLNISIETGQPYITLCHAGIVLGCI
                     PVMDGNIPLGGLFFGKCLWEPLDETIESDVQKRLRGLHIHENEIRQTLLKVPVVSARR
                     IHEASEFLYVLLYQTADLDPQVIQWRRQRSMQQAQISQTIQETKLLDSDEVYPYELEC
                     QLIGKVKIGDRTGAKEILNSLLGRILFHNPGNINLLKARLVELLSVLSRAAAQGGVDI
                     NLLLNKNMDYINKVLALDTQEDICIWISHALDEFIESVYNSQDAQKMSQLKPAIEFMQ
                     YHYSQPLTLADIAKSAHLSVSRLAHLFREQMGVTIVDYLTSIRIQHAKRLLLTSENNC
                     TRICYEVGYNNQSYFTRVFKQLVGMTPREFRRQNKR"
BASE COUNT         2571 a         2727 c         2871 g         2722 t
ORIGIN      
        1 accagttcct gatggttgtg ccggaaccgg caacgctgct gctgctgggt atgggagctg
       61 cggtgttggc tcgcaaacgc tagtttttag taaagcagat tgttttttta gccgaaatcc
      121 tgctgatggc ctcggaaaaa ggccatcagc aggaaatccc aaaaaggagt tgtaacatag
      181 ggtcttaaaa ggggagcaag acaggtagag gacaaaatgc atctatatac atatttttag
      241 gagacatatc tatgaagcag gtacttatgt ttttattggc ccttgcagta tccgttccgg
      301 tttcagcggt tattacccac cggtacgatt ttgaggcaga tgccaatgac cttgtcggcg
      361 gtctcaagac tgtactggaa aatggagcgg tcgtacaaaa tggggctttg gtgctgggcg
      421 gaggttcgcc cggtgctcgt gcagtgctgg atggtcccgg gatagcgatc aatacctata
      481 ccagtgtgtc gattgaagcg tggtttatgg acaatgggct cagcggctgg acgcgggtgt
      541 ttgacttcgg cgatacctcc ggtggagacg gcggctattg tttgttttac acgccttccg
      601 gccccggccg ttcccgactg gctatttcga ccaatggttt ccccagctgg tcaacaggcg
      661 aacagctggt agatggaccc gttcttacat ctgccaccaa ataccatatt gtatgcgtgt
      721 atgaagccgg cggcgggtca ggcggcttga gcgctatgcg tatctatcat aacggggttt
      781 tagccgcctc caatacggct gtttcgatgc ccttgtcagg cctgcataac aattttgcct
      841 atctgggaag tgccgtctat ccgggtgacc ccgaattgaa tgggtctatt tacgaattcc
      901 gcatttatga caaggccctc aatcccggtg aagtgcgctt tacaaatcat ttcggggaaa
      961 acaatgcgta tccgctggtt attcggagtc ataatccgat ttacggccag acgctggttc
     1021 cggtaacgcc cacgctctcg tggacgcctg aagccaatgt tgatgtattg tcctacaatc
     1081 tttatctggg gacagatccc aatattatgg accccaaccg ctctgatgtc agcggcatca
     1141 gcacgctggt cgtaaccaat ctggcaagcc ccagctatac ggttccgtct gcgcagaaac
     1201 tgcttaacaa caaggtatat tattggcgcg ttgatacagt tgcaaccgat ttaaccacct
     1261 atcagggtgc gggaatgacc tttacatcaa ttcctgcatt cccggctttt accgtcaatc
     1321 cggtatccgc gtatgtgttt gaaggccaga cagctgagct gacggcaacc tgccagagct
     1381 taactccgct taccgatgca gtccgctggt acaaggtcgg cacgccggat gtagaagtta
     1441 ccgccggcgg cgatgtgtct attcagactg ttaccgtagg cgacctgacg actacaacgc
     1501 tgagcattgc taatgtggat gcggcaaggg aagccagcta ttatgtcaag gcaaccaatg
     1561 caggcggctc agcggtttca tcttcggcca agattgtcct caagaagctc attgcctact
     1621 ggccgtttga aaacaatctg acggatgcct ccggcaccgg ctatcacggc acagcggttg
     1681 gaaatctgac ctatgcagcc ggcaaggtag gacaagccct tgatttgagc ggcgctaccg
     1741 atacctatgt tgatctgccc gacggatttg ccgactttgt aggcggtttg accttcaacg
     1801 tctgggctta tccgcgctcg gctgccaact gggcacgttt ccttgagttt ggcaatgggg
     1861 ccaatgccga caacatctat tttgcccgca atgggacatc cgcaacactg cagtacacca
     1921 tttataaagg ctcaaccagc tccggtacgg tttcccgcac caatacaatt gctctgaatg
     1981 cttggcagat gtttacgatt acaatggacg ccagcggaag ctgccggctg tacaaggatg
     2041 ggctgcagat tgctaccggt accgttcaga ttcccaatgt ggtaacccgt ataaacaact
     2101 atatcggcaa gagcaactgg gcggctgacg cctattacaa cgggcttctg gatgaaatac
     2161 gcatctacaa ctatgctctg acgccggatg aagtggccga ccggtatgtg gctgatacgg
     2221 gcgcaccgta ctgccgcacc agaccgacct atgacctcaa caacgactgc ttgttcaatt
     2281 tggcagactt tgctatcttt gcagcacagt ggctggattg cggtctgtac ccggcatcgg
     2341 actgcccgaa ttaagggtat cctttctgaa tgttgaaaat cggatttgaa aacagcatat
     2401 gagttttgga ggtataactt atgaaaaaat tgaattcagc agttgtaatt gctctgatgc
     2461 tttccttgtc aataggggca tcagcagcaa cgttgctgtg gtatgatttc gaggacggca
     2521 caccagaaac agtaatgaat ccgggtacgg ctccggcgat tgcttcacgc gatctttctg
     2581 gaaacggcta ccatatgtgg ggttgggcgt ccgggactgc caacaacagt ccgtcttttt
     2641 ctgcgttagg ggataccccg accggttatg gaaccagcgc ccgttatccg gtagccagca
     2701 aagacggcta cacagccggc gctgatgcca tcaatacgtg gtcgccgctt gtctggacaa
     2761 ttgaagtctc tgcaaaattg gattccttgt ccggctggca gacggtgatc ggccggaacg
     2821 gctcttcact gggtgtggct gaggcggatt tctatctgca gaaaaatgac atcgacgacc
     2881 gctggcgcat caacttcggc acggttggcg gggaacgctt tattctcgat tcagattttg
     2941 tggtcgaagc cggaaaatgg taccatattg ctgtggtctc ggatggaact accgttaaga
     3001 tgtatgttga taaaattgac ggcaacggct accagctggt tggttccaga gctttttctg
     3061 gaacgaataa cgctttagca cagggcaatt ttgtctggac cttcggccgc ggctggtaca
     3121 atggcggcaa cgtcgacttt gtcatcggca acattgatga tgtgcggttc agcgatgcgg
     3181 cattgacgcc cgaccagttc ctgcacacga tgtacgattt gggcaatccg tctcccggcc
     3241 atcggtcagt gaatgcacag cgttcgccga ctttgagctg gcagttcatt aatgccggcg
     3301 tcacagcgga ttcttatcag atttacggac ctacaaccga tccgaatctg gttgatccca
     3361 atcagccccg gaccccggac tatacgacga ctgcccagtc gcaggcgatt gccggtccgc
     3421 tggcgtacac gaccaagtat tactggcgtg tggacgcggt tgtcggaacc acggtttata
     3481 ccggaccgat ctggaatttc accacagtcc cgccgtcgcc tgtctttagc gtcaatcccg
     3541 gccgggtagg cgccttcccc ggcgaaagcg ctgttttgac ggccacctgc agcagcacgc
     3601 tgccgctgac ccgtacaatc cagtggttca aggttggcac ccccgatgtg gagattacca
     3661 cagctgatcc ggatgtatca attcagcaaa ccgtcaatgg agaattaaca accagcacct
     3721 tgacaatcag caatatctcg ctggctcacg aagcccaata ctatgcacgg gccaccaaca
     3781 gcggcggatc cgtcaattcg tccaatgggg cgattgtggt caagcggctt ctggcgtggt
     3841 atcagtttga aggcaatacc aacgacagtt ccggcaataa tatgcatgga accgtgatga
     3901 cccccagcgg ggcagcagct acgatcgcct atgtacccag cgtcaacgag gctatgggac
     3961 aggctgtttc ctttacagga acaggaatca gcaacccgac caatacagtt gatccgtatg
     4021 tggacctgcc cgatggcttc tccgatttca gccaagggct gacctttggg gcatgggtct
     4081 atcccacagc agcggccaac tgggcacgca ttgtggaatt tggcaacggg accaacggcg
     4141 ccaacaacag catttacttt acccgtgttg gtaccggtac aaccgtgcgt ctggaaaatg
     4201 acaataccaa tccatccaat aatacttcga ttacaacacc ttccggcggg attaccctga
     4261 acgaatggca gtttttggtc gtaacggtca ataagaatgc gaccaacaat gcgaccatct
     4321 accgcaacgg cagaccgctt gtgacaggga caatcaatct gcccaacatc gtaacccgaa
     4381 ccaactgctt catcggacgc agcgaatggt acggcgattc gctgtatcag ggccgaatgg
     4441 acgatgtgcg gatttacaac tacgggctga cagccgacga aatcgcccag atgtattatg
     4501 atatagtggg cacgttctgt ctgagcaagc cggtctatga ttatgacaac aactgtatcg
     4561 aagatctcgg tgatctggca ataatggcag caacatggct ggattgcggt ctttatccgg
     4621 caacggcctg cccgtaatca gcgggattgg aatttacgct tcagaatgtg tatgtgtttg
     4681 ggctccactc cggcaggatt attcctgccg gagtggcttg cttatatgct attttataaa
     4741 tctgcattgc gggggttaaa atgaagtctg gttcttattt cggtattgtc ctgctcgttg
     4801 cagctgtttt atttgttcag ccggcatcgg cagcgacgat tgcccattgg aattttgagg
     4861 acggtacggc aggtttgcct ttttacaata tgcccagacg cggctcggtc gatattgtca
     4921 acggctatgt catgcgcggc tacaatacga catacggccc gtcgtttaca gccgaaacac
     4981 ctgacggcag cggcttaggc gcctattgca acggcagtca ggacggctat acaaccaatt
     5041 ccgttattaa cggatggtcg ccgcaggcat ggactatcga aatttcagca cggctggatg
     5101 atatttccgg ttgggaaacc ctcatcggcc gcgacggagc aagccacgga gagccggaat
     5161 cggatttcta tctgcagaaa aacaatgtca atggggcctt tcgcatcaat tatgaatccg
     5221 tcggcggaca gcgctggata ttggattcca actttacagt ccaagccggg cgctggtatc
     5281 atttggctgt aaccagcgat ggggcagagc tgacgatgta ttgcgataaa ctggacggca
     5341 gcggctatca ggttgtcggt tcgctggaca tttcctccca gacgcccgcc cagaatgcgc
     5401 tggctgcttc cggtgcaaac tggacattcg gcaggggctg gtacaataac agttttaccg
     5461 atcatattac agggtatttt gataatgtcc gtttcagcga taccgtttta gcgccgaatg
     5521 aatttcttgg ctataatcct attttactga acgaatctga cggcaaaacc ctgttgttca
     5581 agcaagatct ggtgtatacg gatgattaca gcattgttct gaaagaagcc ccggccggtg
     5641 atgtcacggt gacgattacg ccccccgccg gtctgacggt gggaaatgga agcggccaga
     5701 gcttgacgat aacctttacc agtgcagact ggaaccagcc ccgcaccatt acagtgggtg
     5761 tcgccgatgc aggggcactg ctggctgata ttgaatacat tgggcattca gttcaaagca
     5821 ccgatccgaa atttaatggc gtatccatcc gcgatgtggc tgtttacatc gaagaagatg
     5881 cctgcggcgt ctggggctat cttgaaacgg attatgattt caactgcaaa gtcgatctgg
     5941 aagactttgc tgttttagca ggactgtggc tgataaccga tgccccgctc aatctgacgg
     6001 attttgcgac agactggctg atgaatacat ggacgtacaa tgaaaccacc tatgcccgct
     6061 ccattcagcg ttcagaccag ccgttttttg tcaacacggc caatattgcc aacacgattg
     6121 acgagaaaat ctatgggcat tttctggaac atatctacca ttcggtcaat ggcggcctat
     6181 ggggtgaact ggtttggaac cgcagctttg aaatgagcga ttcaggcggc ggcatctggt
     6241 cgattgagga taatcagctt attcaatcca gtttgtcaac cgatgtgcgc ctgccttttg
     6301 gcgatacagg ctggagcgat tatgaattga cccttgaggc ccgaaaggac gggggcagcg
     6361 agggctttct tattccgttc cgctatgccg acggcgataa tttttactgg ctcaatctcg
     6421 gcggatgggg caatgtccgt catgctgtcg agaaggaaat taacggcagc cgaagcacgg
     6481 tttccggaac aactgttaac ggcagcatta atacaggaca gtggtactcc atccgtattc
     6541 gctgtcaagg caatacctac agcatttatt tagataatgt tttgattatc agctttacag
     6601 attccagcaa tcctcatctg accggacaag tgggtgtggg gacatgggct acccaggccc
     6661 gattccgcaa tattcaggtt gcccaaattc ccgattcggc aattttattc agcggtctgc
     6721 cggaccttcc cggaacggcc tttggagcgg cgttctggtc ggttttcgga acagcgcagg
     6781 cgacgatgac aaccgatgct ctcaatgaca gctatgctgt gcagattatt ggtgacggct
     6841 ccgctgccgg cctgatgcag aacaatttca aatttattcc gcagaaatac aacggttctc
     6901 tgtggatgaa ggggtctttg ccggcaggtc tcaaagtgga gctgctggac aatacgaccg
     6961 ttctcggaca ggcaattctt ccggcccctt cggctgactg ggcggaatat ccgtttcaaa
     7021 tagactctgc cggctcaacc aatcagggga tgctccgcat tactctgttg ggtgccggta
     7081 cggttaaaat tgaccagatt agcctgatgg gacaggatgc cgcctctgtc ggcgggtatc
     7141 ggccggatct gctgtcggct gtggaagagc tgcggccgcc gattatccgg tggccgggcg
     7201 gctgctacgc gtctgcgtat ttctggaaag acggcatcgg cccgcagcat cagcgccgca
     7261 agtaccccat cagtctgtgg gatgatcagg ataccaactc ctacgggaca gatgaattcc
     7321 tgcgaatgtg ccgacgcatc ggcgccgaac cgctcatttg tgtcaacacc ggcctgctga
     7381 acggcacctg cggtgtggcg attccttata agctgaccga agaacaatac cttcaggatg
     7441 tcttggactg gatggagtac tgcaatggtc ctgtcgatac gccgtggggc gctgtccgtg
     7501 ctgcgaatgg acatccggag ccgtacaatg taacgtactg ggaaatcgat aatgaaacat
     7561 ggtcaaccag ctggggcggc ggcataacca actatattgc caaagtccag ctgtttgcgc
     7621 cggcaatgcg gatcaaggcc gaacagctgg gcgttcctgt caagctgatt gcctgcggca
     7681 gcggcagcta tgatcagtcc tggaaccgta cgctgattga taactgtgcc gtgctgattg
     7741 actacattag cacgcaccat tacgaatcgc cggacaacta taagcaggga ccccgcagct
     7801 atgaagcctt tatcaaagaa ctggctgcct atattgccgc ctccgccaat ccggatttga
     7861 agatttatat gtcggaatgg aatgcccaga gcaccgactg gcggaccggc ttgtatgctg
     7921 gcggcctgct gaatgctttt gagcgcaccg gagatgtttt tgaaatcggc ggtccggctc
     7981 tcttcttgcg gcatacgtcg gcttccggat gggataatgc ctttatcaac tttgaccata
     8041 ccggctggtt tgccgctccg aattatattg ttatgaagct gtggcatgac cattacgctc
     8101 ctctgtgcgt ccaaaccaac gggacagaca ccaatcttaa tgtggtttca gctctttcag
     8161 cagacgaaaa aacactgtat atccgaattg ttaatccgga tgcagatagc aaatcggtcg
     8221 cctttgaaat cgatccgtcc tttacggctg aatcggccta tatgcattat gtggcgccgg
     8281 gcagtttgta tgcccgcaac acattggcta atccgcatgc agttcgtgtt gaagccaagg
     8341 tggttggtct ggatgaccag acattgcggt ttaagatgcc gggttatgcg gctgctgtgg
     8401 ttacggtccg gacgtccaaa ccgcacaaga cagcgttttt gtacagttcg tttcgcaata
     8461 acggcgaaga tgggcttcat ctggcataca gtcaagacgg cctggtgttt actgccttga
     8521 aaggcgacac atcatttctt gctccgcagg tcggcagccg gctgatgcgc gacccaagca
     8581 tctgtcaagg ccccgatgga atgtttcatc ttgtctggac aaccggctgg tgggataagg
     8641 gcattggcat tgcccattcg cccgatttga tcaactggtc ggagcagata tttctgcctg
     8701 tgatggccca tgagccgaat gccaaaaact gctgggctcc agagatcttt tacgatgagg
     8761 caacggaccg ttttctcatc ttttggtcca ccacaatcga cggcgcattt ccagagacct
     8821 ataatcccag cgatgacaac aaccatcgca tttactatgt ttctacgcag gattttgtaa
     8881 cctatagcaa tacagccctg ttttatgatc ccggttttaa ctgcatcgat gccttcatcg
     8941 ccaagcatgg cgctggttat gtgatgtttc ttaaaaatga gaccaaagtg ccgattgccc
     9001 agaaaaacat ccgtatggct ttcagtgact ctgccgcagg accctgggga cctgcttccg
     9061 aatcgatttc cccagcttgg gtagaaggac cctcagctgt aaaggctggc ggacagtggt
     9121 atctgtacta tgatgcctat acgcgagggc gaatggaggc aacggcttca ctggacctga
     9181 aggcctggac cgatattact agccagctgt catttcctgc aggtacacgc catggaactg
     9241 tttttcgtgt tgcacaggat atcgtggacg ctcttaaatc gctctaaatt gtcagggata
     9301 cagcagcggc acccattgaa caagaccccg gctgttaagc cggggttttt ttatcgcttg
     9361 ttctgccggc ggaattcccg cggtgtcatg ccgacaagct gcttgaaaac gcgggtaaaa
     9421 taactctggt tgttatagcc gacttcgtag caaatacggg tgcagttgtt ctcagaggtc
     9481 agcagcagcc gtttggcatg ctggatgcga atactggtca gatagtccac aatcgtaacg
     9541 cccatctgtt cgcggaaaag gtgggccaga cgcgatacgc tcaaatgggc gcttttggcg
     9601 atatctgcca gcgtcagcgg ctgcgaatag tggtactgca taaactcaat agcgggcttg
     9661 agctgggaca tcttttgggc atcctgtgaa ttgtagacgc tttcgataaa ttcatcgagg
     9721 gcgtggctta tccagatgca gatgtcttcc tgtgtatcca gcgccaaaac tttattgata
     9781 taatccatat ttttattgag cagcaggttg atatccacgc cgccctgggc tgccgctcgg
     9841 ctcaatacgc taagcagttc gaccagacgg gctttgagga gattgatatt gccgggattg
     9901 tgaaacaaaa tgcggcctaa cagggaattc aatatttctt tggcccccgt tcggtcgccg
     9961 attttgacct tgccgataag ctgacattcc agctcatacg gatagacctc atccgagtcc
    10021 agtagtttgg tttcttggat ggtttggctg atttgggcct gctgcatcga acgctgccgc
    10081 cgccactgaa tgacctgcgg atccagatcg gctgtctgat acagtagaac gtaaagaaat
    10141 tctgaggcct cgtgaatacg ccgggccgag accaccggaa ccttcagcag cgtctgacga
    10201 atttcattct catggatatg aagtcctcgc agacgcttct gtacatccga ttcaatggtt
    10261 tcatccaacg gttcccaaag gcatttgccg aaaaacagac ctcccaaagg aatattgccg
    10321 tccatcactg gtatacagcc caaaacaatg cctgcatggc agagagtgat gtagggctga
    10381 ccggtttcaa tggagatatt taaactgcgg acacggtcct ctctgcatcg cttcaggcct
    10441 gttttagaaa gacgaatccg ctgacagaat gccggatgac agtttgaact gcagagagac
    10501 cgtatttctt ggccatttat atctgttgtc tcaactggaa gtttgaaatt ccgcaaaaag
    10561 ccggcggcaa tccttccgaa ctgttcttta gaaataatat cttgtattcg aacagattgt
    10621 agatttagct ttttcatttc caatttctcg attggcctaa caatatcata gcaaaatagt
    10681 attatagaaa atttaatttt aagggcaaga agtatgatta gaaaaagaat acaatgcaat
    10741 atgtatatct gtaatatcca gatattcgcc attcaaagaa aaacttgctg aactaataaa
    10801 agggcgcagt attttcgcat tttacccttt tacgatttgt ccgaataaag gatatctgca
    10861 aattgtctat tgtgaagatt tctttaatcg g
//
LOCUS       DAOTBO010000091        10678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5282_length_10678_cov_5.443472, whole genome shotgun sequence.
ACCESSION   DAOTBO010000091 DAOTBO010000000
VERSION     DAOTBO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5282_length_10678_cov_5.443472"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1270
                     /locus_tag="PLE88_09955"
     CDS             182..1270
                     /locus_tag="PLE88_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76827.1"
                     /translation="MANYYNMQEVMNKLRKTEQEVQELVRQGQLRQYLDAGKPVFNVD
                     DVNALAEEIVGLDITSAGLNLEEGSDLEIKLEETSEIRLEPDEGPAGDKGAADLELDF
                     ADEQPKAKESEGGFGLSQMGDLSSGDTNVGSVGINILSGTEDAYKLTEDTKAETQAAD
                     EVESLDADANLESFGSGSGLLDLSLQADDTSLGAVLDDILPAGAEGAGGEAPAAAGEP
                     PIGLAEEPEDLMAPAEAPAEAAAPLAAEAAVPVGPVGAGAVPLAAAVPADPRTNMYGV
                     AMFLPAAALVLAAVVLGAAVQDVVPSLAKILTKTMIADISLIWIISIGLILLVILLSL
                     AAAMGGQGGSKKEKAPKPKKEKPAKKRK"
     gene            1372..2154
                     /locus_tag="PLE88_09960"
     CDS             1372..2154
                     /locus_tag="PLE88_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOM76828.1"
                     /translation="MNRGTGLVWAACCIGLAALTGCQNQRKLDDLQTQNRIQQQRIEQ
                     LENELSQCTGQLQQKLQELQALAGRSGADVQAKDALIAALEADLEKKKALIAQMQNQL
                     MQGGTPLPAEMNVLLQDFAKTSDMIDFDASTGMLKFKSDLLFNLGSDQVQASAAESLK
                     ALAGIMNTAEGKQFDMVIVGHTDDVPIKKAETLRLHPTNWHLSVHRAISVEEMLSKNG
                     IAPERMAVKGYGEYRPVEPNKPNKGGNEANRRVEIFIIPGSS"
     gene            2191..2652
                     /gene="ndk"
                     /locus_tag="PLE88_09965"
     CDS             2191..2652
                     /gene="ndk"
                     /locus_tag="PLE88_09965"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOM76829.1"
                     /translation="MPQRTLIIIKPDAVQRQLVGRIISRFEDKGLKIAAAKFMQISED
                     LARRHYAVHAGKPFFEGVVRYLASSPVLVMVLEGDGVIEMARKLMGKTFGNEAEPGTI
                     RGDFGASRGYNLVHGSDSPESAAYEIGLYFRPDEIVDYSLAAQAWIYGSNE"
     gene            2699..2881
                     /locus_tag="PLE88_09970"
     CDS             2699..2881
                     /locus_tag="PLE88_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76830.1"
                     /translation="MDGRAAEYLLPQEAGQTGLPERAHPVFNLPVDFAGGVLLQPVPR
                     WADVFQMDLPIQQAGE"
     gene            complement(2909..3250)
                     /locus_tag="PLE88_09975"
     CDS             complement(2909..3250)
                     /locus_tag="PLE88_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76831.1"
                     /translation="MRLTVAVCGIIGLLCGLADSGQPVRIGPADRNGAVRMPAEQFLK
                     SHSFKSEYKMKIIPPDCRIHPKITVIRPESGIDYKTRIYDPYLKKWRQNGAEQGPLHK
                     RWPGLFIKPEQ"
     gene            3400..5400
                     /gene="ligA"
                     /locus_tag="PLE88_09980"
     CDS             3400..5400
                     /gene="ligA"
                     /locus_tag="PLE88_09980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOM76832.1"
                     /translation="MKDIRKQIERLREEIRRHDVLYYVHNAPEISDREYDNLFAELKR
                     LEKEHPELITPDSPTQRVSEQPVEGFATVAHAVPMLSIDNTYSEGELRDFDRRIAKGL
                     EGGDYEYTVELKIDGLAVSLRYEKGRLVQGATRGDGRRGDDVTANIRTIRAVPLTLEG
                     DGLPDVLEIRGEVYMSKKAFAQLNAAKAAAGEPEFANPRNAAAGSLKLLDARITASRK
                     LSFFAYALGQVSRPLAQTHYESLQKLRSFGLPVNPHTQKAANIDEVIAICRSWEGRKD
                     KLDYPIDGMVIKVNRYDQQDRLGLTGRAPRWCIAYKFAAEQAETVVESISVQVGKTGI
                     LTPVANLKPVKLAGTTVKRASLHNFDLLQKLDVRCGDTVIIEKAGEIIPQVVDVKIQQ
                     RHLFDSRPFEIPTACPACGGRVKKDDSGVYLRCVNADCPAQLKERLEYFAGKGQMDID
                     TLGPALIDQLIRAGLVKSFADLYRLRFDQLVQLERMGDKSAANVLDSIEKSKHADLWR
                     LIAALGIRNVGGQTAQILADEFGSLEALMNASVERLTEIEQIGPVVAESIYEYLHNPA
                     NIRVIRQMLAAGVSPTPPKPKASAVLAGQTIVVTGTLKHFTRQQIEQTIKDHGGKTSS
                     SVSKKTSFVLAGQDPGSKLEKARRLGVEILDEDQFLRRIGAV"
     gene            5455..6837
                     /gene="miaB"
                     /locus_tag="PLE88_09985"
     CDS             5455..6837
                     /gene="miaB"
                     /locus_tag="PLE88_09985"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOM76833.1"
                     /translation="MQKPKIHLKNFGCQMNKLDASLLGSALAAQGYAFTDSPAEADVI
                     VLNTCSVRQHAEDRVLSHLGHLRHLKKSRPQLLVAVIGCMAQRLGRALLAHEAVDIVA
                     GPSQLPEVPALIAQALVDRGKYLSVSEKIRQTDEADEDRLDAFEYAHDSDKLHLKSQA
                     FIRVMRGCNNFCSYCIVPYVRGPEVSRPPQVIVEQAHKLAAEGIQQITLLGQTVNSYR
                     YTDGGKTYTLTDLLRQVSAIEGIHWVRFVTSHPGKFDESILELMADNPKVCPYLHIPA
                     QSGSDRILQAMNRGYTARQYAALIEKARRIVSDIAIAGDFIVGFPGETEEDFQASLDL
                     IRRVRYKNAFVFKYSPRPGTLADKRLADTVPAEVKQQRNIRMLEVISEIAEEDNQRFV
                     GKTVEVLVEGPSKKPHLNQAADADHPQLIGRTADDYIAVFNGPASLTGRFVHVCIEKT
                     AALTLFGRLI"
     gene            complement(6834..7388)
                     /locus_tag="PLE88_09990"
     CDS             complement(6834..7388)
                     /locus_tag="PLE88_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76834.1"
                     /translation="MICRMLQWAMEGQLDRHGAIQSPPLLRHVQHCPACSRWLQTHQQ
                     LQRRLQHPLPSPVSPARISRLQARLQQRLSEAGRIRKAPFRPAALQSAAAVLLLGAGI
                     GLLYVYYAPTAPVSSGAADALQLITPAAGWQQIPQWAALSEQPLRTEVSNLAADARRA
                     MQFLVTCLPAGGVPAGLSEAAAPD"
     gene            complement(7390..7971)
                     /locus_tag="PLE88_09995"
     CDS             complement(7390..7971)
                     /locus_tag="PLE88_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76835.1"
                     /translation="MNAFSAQSDQAVARAAAAGDRAAFDEIVRRYMRPLLEFAAAKTS
                     VPNAEDIVQETFLRAYQNITAYSDRYTLKSWLFTIAWRQIISLYRKKQPDLLTDEPAW
                     RPAESADPPDAEWLWAAARTMGQDYYTVLWLRYQQQMTTDEIAQVMRKSQIGVRVLLH
                     RARKRLGQMIAQTTSIPIGRPRKSAAALAERTS"
     gene            complement(7993..8928)
                     /locus_tag="PLE88_10000"
     CDS             complement(7993..8928)
                     /locus_tag="PLE88_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76836.1"
                     /translation="MKTLVYYAIGAILLSGYAAAAPLNKAHISGDALWLVHLDLNAFK
                     SSQFGRLALGEITQRQQQKIDALTQLLGTDLTRDIDAVTLYGPDAAQENAVALLYGRF
                     DRNKLLTLLKLNDAYAESTYKNHLLYHWKDDKKQTAQVGAFWTDNCIVISQTESAVGT
                     ALDVLAGQAGSLAANPHSVLYELTNAPAGTILLTAAQSLTELTRNNPHAAILQNSSIM
                     AVVAGESSGIMKLQIRLDAASAEAAGQIETIVRGIMAFGLLQQDKNPKLAPLMNAVTL
                     TRQDNRLELVFQYSSAELFEMAKADISHIELPAAL"
     gene            9143..10222
                     /locus_tag="PLE88_10005"
     CDS             9143..10222
                     /locus_tag="PLE88_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76837.1"
                     /translation="MMKIQRITDLVLLVFWAVLPAAWSAPERYFPDIAGYQTLVGDFH
                     THTVFSDGLVWPTVRIQEAVREKLDFIALTDHIEYQPHKQDIPTNHARPYEIAQPVAQ
                     KNGILLIRGAEITRDTPPGHYNAVFLTDIQPLQTESLLDALQRANAQKGFVFWNHHAW
                     QGAERGQWQAVQTEMYEKGWLHGLEVANGDDYYPQAHQWCLEKKLTMLGNSDIHDPSS
                     FSPYSAGRHRTATLVFAAEKSVPAIQQALAEGRTVVWYKNQLIGREEFLIPLLEASVE
                     TESRWTPQQTLAVTVHCRAMIELELDVSGRHGTTAFTVPAFGHKTAEFFVEPQQDWLV
                     LDYCVKNFLIKPGQGITGQIRFQKP"
     gene            complement(10327..>10678)
                     /locus_tag="PLE88_10010"
     CDS             complement(10327..>10678)
                     /locus_tag="PLE88_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOM76838.1"
                     /translation="LKNQADQLVYSTEKMLKEHGDKVPANVRSDIESAVNTLKEAAKG
                     EDGQAIKRAMEKLAETSQQLGKILYEEAAKKQAASGAGAGSSASGTQAGPAEGEVRRK
                     GEGDVIDAEFETKD"
BASE COUNT         2293 a         2843 c         3266 g         2276 t
ORIGIN      
        1 ttgggcgagt cggcttggtc tgccggctgc tgggaagaat ctgctgccgc cggcagtgcg
       61 taaaggggcg tttgcaaagg tgttaaaccc taaaaaaaag acttttcaag cgtccggcag
      121 gccgatataa tgattggaga gcgatacata gccatactaa aacatgtttg ggaagaaaat
      181 catggcaaac tattacaaca tgcaggaagt aatgaacaag ctccgcaaga ccgaacagga
      241 ggttcaggaa cttgtcaggc agggtcagct gcggcagtat ctggatgcgg gcaagccggt
      301 gtttaatgtc gatgatgtca atgcactggc tgaagaaatc gtcggacttg atattacttc
      361 agccggcctg aatctggaag aaggttcgga tctggaaatc aagctggaag aaacttcgga
      421 gattcggctg gaaccggatg aagggcctgc aggggataaa ggggctgcgg atttggagct
      481 ggactttgcg gatgaacagc ccaaagccaa ggaaagcgaa gggggatttg gtctgagcca
      541 gatgggcgac ctcagcagcg gggacacgaa tgtcggctct gtcggcatca acattctcag
      601 cggcacggaa gatgcctaca agctcaccga agacaccaag gccgagacgc aggcggccga
      661 tgaagtcgaa agtctggatg ccgatgccaa cttagaaagc ttcggcagcg ggtcaggatt
      721 gctggacctt tcgctgcagg cggatgatac ctcgctgggg gcggtgctgg acgacattct
      781 gcctgccggt gccgaaggcg ccggcggcga agctcccgcc gcggctggcg aaccgccgat
      841 cgggctggca gaagaacccg aagacctgat ggctccggct gaagcgccgg ccgaggcggc
      901 tgcaccgctt gcggcggaag cggcagttcc cgttgggcct gtcggggcag gggctgtccc
      961 gctggcagcg gcggttccgg cggatccgcg gacgaatatg tacggcgtag cgatgtttct
     1021 gccggccgcg gctctggtgc tggcggctgt cgtgctgggt gctgcggtgc aggatgtggt
     1081 gccgtccttg gccaagattc tgaccaagac gatgattgcc gacatcagcc tgatctggat
     1141 tatttccatc ggattgatac tgctggtgat tcttctgtcg ctggctgctg ccatgggcgg
     1201 tcagggcggt tccaagaagg aaaaggcccc gaagccgaaa aaggaaaaac cggccaaaaa
     1261 gcgcaaataa tcaaatgttt cggcctccga aagccgacat aaaaaaacgg atggacatcc
     1321 actgtatagg tgcaaacaat taacggattg atagttcgaa aggggattgg aatgaacagg
     1381 ggcacaggtt tggtatgggc ggcatgctgt attgggctgg cggcgctgac gggctgtcag
     1441 aatcagcgga aactggatga cttgcagacg caaaaccgca tccagcagca gcggattgaa
     1501 cagctggaaa atgaattgag ccagtgcacc ggccagctgc agcagaagct gcaggaactg
     1561 caggccttgg ccggcaggag cggggcggat gtgcaggcca aagatgcgct gatcgccgct
     1621 ctggaagcag accttgaaaa gaagaaggcc ctgattgccc agatgcagaa tcagctgatg
     1681 cagggcggca cgcctctgcc ggcggaaatg aatgtcctgc tgcaggattt tgccaagacc
     1741 agcgatatga tcgatttcga tgcctctacc ggcatgctga aattcaagag cgacctgctg
     1801 ttcaatctgg gcagcgatca ggtgcaggcc agtgcggccg aatcgctgaa agccttggcc
     1861 ggaattatga ataccgccga aggcaagcag tttgatatgg tgattgtggg ccataccgac
     1921 gatgtgccga tcaagaaggc cgaaaccctt cgcctgcatc cgaccaactg gcatttgtcg
     1981 gtgcatcggg ctatcagcgt cgaggagatg ctcagcaaga acggcatcgc ccccgagcgg
     2041 atggctgtca agggctatgg cgaataccgt ccggtggagc cgaacaagcc caacaagggc
     2101 ggcaatgaag ccaaccgccg cgtagaaatc ttcatcatcc ccggcagctc gtaagcctgc
     2161 gggtgcctcc ttggtgtaaa aggcagaccg atgccccagc ggacgctgat tatcatcaag
     2221 cccgatgctg tccagcggca gcttgtcggc cggattatct cccggtttga ggacaaggga
     2281 ctcaaaatcg ctgccgccaa gtttatgcag atttccgaag atctggcccg ccggcattat
     2341 gcagttcacg ccggcaagcc gttttttgaa ggcgtcgtgc ggtatctggc gtcgtcgccg
     2401 gttctggtga tggtgctgga aggcgacgga gtgattgaaa tggcccgcaa gctgatgggc
     2461 aagaccttcg gcaatgaggc cgagccgggc acgatacgcg gcgacttcgg cgccagccgc
     2521 gggtataatc tggtgcacgg ctccgacagc cctgaatcgg cggcgtatga aatcggtctg
     2581 tatttcaggc cggatgaaat tgtggattac tccttagccg cacaggcgtg gatttacggc
     2641 agtaacgaat aagagttctc agaatgacag aagcccccgt gtgtcagggg gcttttttat
     2701 ggatgggcgg gcggctgagt atctgctgcc gcaggaagcc ggccagacgg gtctgccgga
     2761 acgggcgcat ccagtcttca atctgccggt ggattttgcc ggcggcgtat tgctccagcc
     2821 ggttccgcga tgggctgatg tctttcagat ggatcttcct atccagcagg cgggcgaata
     2881 agtgtccggc ggtctctggg gcaaagtctt attgttccgg cttgataaac aggccgggcc
     2941 atcgtttgtg cagcgggcct tgttctgccc cattctgccg ccattttttg agatagggat
     3001 cgtaaatcct ggttttgtag tcgatgcccg attcaggacg aattacggtt attttaggat
     3061 ggatgcggca atccgggggg atgattttca ttttatactc ggatttgaac gagtggcttt
     3121 tgaggaattg ttctgccggc atccggacag cgccgtttct atctgccggc ccaatccgga
     3181 cgggctggcc ggaatctgca aggccgcaca gcaagccgat tatcccgcat accgcaacag
     3241 tcaatcgcat agcaggtctc ctgccgagat ttcctgatga tttacttcct gttatttaga
     3301 tgtataatac catttgatgt tcccttgctg gtataaaagt tttgtaaaaa ccagcgtgtt
     3361 tcggtgcggt ctttcgccgc agacagagga aagtatctga tgaaggatat ccggaaacaa
     3421 attgaacggc tgcgggaaga gattcgccgg catgatgtgc tgtattatgt ccataatgcc
     3481 cctgaaatca gcgatcggga atacgacaat ctgtttgcag aattgaagcg tctggagaag
     3541 gaacatccgg aattgataac tccggattca ccaacgcagc gggtgtccga acagccggtc
     3601 gagggatttg caacagtcgc ccatgctgtc ccgatgctga gcatcgacaa tacctacagc
     3661 gaaggggagc ttcgggattt tgaccggcgg atagccaaag gtcttgaagg gggtgattat
     3721 gaatatacgg tagaactgaa aatcgacggg cttgccgtca gtttgcggta tgagaaaggc
     3781 cggctggtgc agggagccac ccgcggcgac gggcggcggg gggatgatgt taccgccaat
     3841 atccgcacga ttcgtgcagt gccgctgacg ctggaaggcg atggcctgcc ggatgttctt
     3901 gaaatccgcg gcgaggtgta tatgtccaag aaggcctttg cccagctcaa cgccgccaag
     3961 gccgccgccg gcgagccgga gtttgccaat ccgcgcaatg ccgccgccgg gtcgctgaag
     4021 ctgctggatg cccgcatcac cgccagccgc aagctgtcgt tttttgccta tgcgctgggg
     4081 caggtcagca ggccgctggc ccagacgcat tatgaatcgc tgcagaagct gcgttcgttc
     4141 ggcctgccgg tcaatcccca tacccaaaag gccgccaaca tcgatgaggt catcgccatt
     4201 tgccgctcgt gggaaggccg caaagacaag ctggactacc cgattgacgg aatggtcatc
     4261 aaagtcaacc gctatgacca gcaggatcgg ctgggcctga ccggccgggc gccgcggtgg
     4321 tgcattgcgt ataaatttgc tgccgaacag gccgaaacgg tggtcgagtc catcagcgtg
     4381 caggttggca agacagggat tctgacgccg gtggccaatt tgaagccggt caagctggcc
     4441 ggtacgaccg tcaagcgtgc gtcgctgcac aatttcgatt tgctccagaa gctggatgtc
     4501 cgctgcgggg atacggtgat tattgaaaag gccggtgaaa tcattccgca ggttgtcgat
     4561 gtcaaaatcc agcagcggca tctgtttgac agccggccgt ttgagattcc gacggcttgt
     4621 ccggcctgcg gcggcagggt caaaaaggac gacagcggcg tctatctgcg gtgcgtcaat
     4681 gccgactgcc cggcacagct gaaggagcga ctggaatatt ttgccggcaa agggcagatg
     4741 gatatcgaca cactgggtcc ggctctgatt gaccagctta tccgtgccgg actggtcaaa
     4801 agttttgccg acctctaccg gctccgtttt gaccagctgg tgcagctgga acggatgggc
     4861 gacaaaagcg ctgccaatgt gctggacagc atcgaaaaga gcaagcatgc cgacctgtgg
     4921 cggctgattg cggcgctggg catccgcaat gtaggcgggc agacagcgca gattttagcc
     4981 gacgagttcg gctctctgga agccctgatg aatgcctctg tcgagcgtct gaccgagatc
     5041 gagcagattg gaccggtggt ggctgaaagc atttatgaat acctgcacaa tccggccaat
     5101 atcagggtta tccgtcaaat gctggccgcc ggtgtcagcc ctacgccgcc gaagcccaaa
     5161 gcgtccgctg ttctggccgg acagaccatt gtggtgaccg gcacgctcaa gcacttcacc
     5221 cgccagcaga tcgaacagac catcaaagac cacggcggca aaacctcctc ctccgtcagc
     5281 aaaaagacca gttttgttct ggccggtcag gatcccggca gcaagctgga aaaggcccgc
     5341 cggctgggcg ttgaaatcct tgatgaggac cagtttctcc ggcgcatcgg ggctgtctga
     5401 tcgggcagaa cggctcttgg atttaaagcg aaggtatgct atactgacgg gtctatgcag
     5461 aagccaaaga ttcatcttaa aaatttcggc tgccagatga acaagctgga tgcgtcgctg
     5521 ctgggttcgg cgctggccgc gcaggggtat gcgtttacgg acagccccgc cgaggcggat
     5581 gtgattgtgc tcaatacctg ctcggtgcgc cagcatgcag aagaccgcgt gctgtcccat
     5641 ctgggacatc tgaggcatct taaaaagagc cgtccgcagc tgcttgtggc cgttatcggc
     5701 tgtatggccc agcggctcgg ccgtgcgctg ctggcccatg aggcggtgga cattgtggcc
     5761 ggccccagcc agcttccgga ggtgccggct ctgattgccc aagcgctggt cgaccgcggg
     5821 aagtatttgt ctgtatcgga aaaaatccgt cagaccgatg aggccgacga agaccggctg
     5881 gatgcgttcg aatacgccca tgactcggat aagctgcatc tgaaatcaca ggcgtttatc
     5941 cgggtgatgc gcggctgcaa caatttctgt tcgtactgca ttgttccgta tgtgcgcggc
     6001 ccggaagttt cgcgtccgcc gcaggtgatt gtcgaacagg cccacaagct ggccgctgaa
     6061 ggcattcagc agattacgct gctgggccag accgttaatt cctaccgtta tacagacggc
     6121 ggcaaaacct ataccttaac ggacttgctc cggcaggtca gcgccatcga agggattcac
     6181 tgggtgcggt tcgtaaccag tcatccgggc aaatttgatg agtccatact ggagctgatg
     6241 gcggacaatc cgaaagtctg cccgtacctg catattcccg cccaaagcgg ctcggaccgg
     6301 attctccaag cgatgaaccg gggctataca gcccggcagt atgcagcgct gatagagaaa
     6361 gcccgccgca tcgtttcgga tatcgccatt gccggcgatt ttatcgtggg ctttcccggc
     6421 gaaacggaag aggattttca ggcgtcgctg gatttaatcc gccgcgtccg ctacaagaac
     6481 gcctttgttt tcaagtattc gccccgtccg ggcacgctgg ccgataaacg attggccgat
     6541 accgtcccgg ccgaggtcaa gcagcagcgc aatatccgca tgctggaagt catcagcgaa
     6601 atcgcagaag aagacaatca gcggtttgtc ggcaagaccg tcgaagtcct cgtggaagga
     6661 cccagcaaaa agccgcacct gaatcaggca gccgatgcgg atcatcccca gctgatcggc
     6721 cgtaccgctg acgactacat tgcggtattt aacggaccgg cttccctgac cggccggttt
     6781 gtccatgtct gcatcgaaaa aaccgccgcc ctgacgctgt ttggccggct catttaatcc
     6841 ggcgctgcgg cctctgacag cccggccggt acgccgccgg cgggcaggca ggtgaccaga
     6901 aactgcatgg cacggcgggc atcggcggcc agattgctga cctcggtgcg gaggggctgt
     6961 tcggacaggg ccgcccattg gggaatctgc tgccagccgg ccgcgggggt tatcagctgc
     7021 agcgcatcag ccgccccgga ggagacgggg gccgtcgggg catagtaaac atacaaaagc
     7081 cctatcccgg cgccgagcag aagcacagcc gcggcggatt gcagagccgc cggccgaaac
     7141 ggcgcctttc ggatgcggcc ggcttccgac agccgctgct gtagccgggc ctgaagccga
     7201 cttatccgag ccgggctgac cggacttggc agcggatgct gcagccgccg ttggagttgc
     7261 tggtgcgttt gaagccagcg gctgcatgca gggcagtgct gcacatgccg cagcagaggg
     7321 ggactttgaa tcgctccgtg acgatccagc tgaccttcca tagcccattg aagcatccgg
     7381 catatcatat cagcttgtcc tttctgccag cgctgccgct gatttgcggg gccggccaat
     7441 cggaatggag gttgtttggg caatcatttg tcccagccgt tttctggcgc ggtgcagcag
     7501 aacccgcacg ccgatttgcg attttcgcat cacctgcgca atttcatccg tggtcatctg
     7561 ctgctgatac cgcagccaca gcaccgtgta gtagtcctgt cccatggtgc gggctgctgc
     7621 ccagagccat tcggcatccg gcggatcggc agattcagcc ggccgccaag ccggttcgtc
     7681 cgtcagcaaa tcgggctgtt ttttccggta cagcgagata atctgccgcc atgcgatcgt
     7741 aaacagccag cttttaagcg tatagcggtc gctgtaggcg gtaatgttct gatacgcccg
     7801 cagaaaggtc tcctgcacaa tgtcttcggc attcgggacg gatgttttgg ctgcggcgaa
     7861 ttccagcaga ggccgcatat agcgccgcac aatttcatca aaagcagccc ggtcgcctgc
     7921 tgcagcggca cgggctacgg cctgatcgga ttgtgctgaa aaagcattca tttcgatatc
     7981 cagccggagg catcagagag ccgccggcag ttcgatatgg gaaatatcgg ctttggccat
     8041 ttcaaacagc tcggcggagc tgtactgaaa gaccagttcc agccggttgt cctgccgggt
     8101 cagtgtgacg gcgttcatca gcggcgccag cttgggattt ttgtcctgct ggagcagacc
     8161 gaaggccatg attccgcgga cgatggtctc aatctgtccg gcggcctctg ccgaggcggc
     8221 atccagccga atctgcagtt tcatgatgcc ggatgattcg ccggccacca cggccataat
     8281 gctggagttc tgcagaatgg cggcgtgcgg attattccgg gtcagctccg tcagggactg
     8341 ggcagcggtc agcagaatgg tgccggcggg ggcattggtc aattcataca gcacagaatg
     8401 gggattggct gccagcgatc cggcttgtcc tgccagcaca tccagcgccg ttccaacggc
     8461 cgactccgtc tgcgagataa caatgcagtt gtctgtccag aaggccccga cctgagccgt
     8521 ttgcttcttg tcgtctttcc agtgatacaa aagatggttc ttgtaggtgc tttcggcata
     8581 ggcgtcgttg agtttgagca gggtcagcag tttgttccgg tcaaagcggc cgtacagcag
     8641 ggccaccgca ttttcctgag cggcatccgg cccgtagagg gttactgcat ctatatcccg
     8701 cgtcaggtct gtgcctaaaa gctgggtcag ggcgtcaatt ttctgctgct ggcgctgcgt
     8761 gatttccccc aatgccagcc ggccgaactg gctggattta aaggcattca aatcaagatg
     8821 aaccagccag agggcatcgc ccgaaatatg ggctttgttc agcggcgccg ctgcggcgta
     8881 tccagaaagc agaatagccc ctattgcata atacacgagt gttttcattg tcctgtcctt
     8941 tccgattgta aggttcattg tacgcttttg cgtttcacca agcaaggatt ccttgtttgc
     9001 ctgttattac gcaaagccgt tgggaaatgt tacagacaat tggattttta tctgaagtag
     9061 cggaaataaa gggtgccgga tatgcgttag cgagtataaa gacggcagat atcggcaaaa
     9121 tcgattggtg tgcgggagat ttatgatgaa aatacagcga ataacggatt tggtgcttct
     9181 ggttttttgg gcggttttgc cggcggcttg gtcggctccg gagcggtatt ttcccgatat
     9241 tgccggctat caaacactgg tgggcgattt tcatacccat acggtttttt ccgacggact
     9301 ggtctggccg actgtgcgga ttcaggaagc cgtgcgggag aagctggatt ttattgccct
     9361 gaccgaccat attgaatacc agccgcacaa gcaggatatt cccaccaacc atgcccggcc
     9421 gtatgaaatc gcccagccgg ttgcacagaa aaacgggatc ctgctgattc ggggggcaga
     9481 aattacccgg gataccccgc ccggacatta taatgccgtt tttctgaccg atatccagcc
     9541 cctgcagacc gaatcgcttc tggatgccct ccagcgcgcc aatgcccaaa agggctttgt
     9601 tttctggaat caccatgctt ggcagggcgc agaacgggga caatggcagg ctgttcagac
     9661 cgagatgtat gagaaaggat ggctgcacgg gctggaagtg gccaatgggg acgattacta
     9721 tccgcaggcc catcagtggt gtctggaaaa aaagctgacg atgctgggca attcggatat
     9781 ccacgatccg tcttcttttt caccctattc ggcaggccgg caccgcacgg caacgctggt
     9841 ctttgcagca gaaaaaagtg tgccggctat tcagcaggcc ctcgcagaag gccgcacggt
     9901 tgtctggtat aaaaatcagc ttatcggcag ggaagaattt ctgattccct tgctggaagc
     9961 atctgtggaa accgagtcgc gctggacgcc gcagcaaacc ttggctgtaa ccgttcactg
    10021 ccgggcgatg atagaattgg agctggacgt atccggccgg cacggcacaa cggcttttac
    10081 ggtgccggcg ttcggccata agacagccga gttttttgtt gagccgcagc aggactggct
    10141 ggttctggat tactgtgtga agaattttct gattaagccc ggccaaggca ttacgggcca
    10201 aatccgcttt cagaagccgt aagcggcgct ctgctgaagc gttccggcag atttgccggc
    10261 gcggttggac agtccataaa aaaagccctg cgttggacag ggcttttcag tgggcggaag
    10321 aaagatttag tctttcgttt caaattcggc atcgattaca tcgccttcgc cttttctgcg
    10381 aacctcgcct tcggcggggc ctgcctgcgt gccggaagcc gaactgccgg ctccggcgcc
    10441 ggaagctgcc tgctttttgg cagcttcttc atagagaatc ttgcccagct gctgggaggt
    10501 ctcggccagc ttctccattg cgcgtttgat ggcctgccca tcttcgcctt tggcggcttc
    10561 cttgagggta ttgaccgccg attcgatatc gctgcgaaca ttggccggaa ccttgtcgcc
    10621 atgctccttg agcattttct cggtggaata gacaagctgg tcggcctgat tcttcagg
//
LOCUS       DAOTBO010000092        10553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5356_length_10553_cov_5.989712, whole genome shotgun sequence.
ACCESSION   DAOTBO010000092 DAOTBO010000000
VERSION     DAOTBO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_10553_cov_5.989712"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2256)
                     /locus_tag="PLE88_10015"
     CDS             complement(<1..2256)
                     /locus_tag="PLE88_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOM76839.1"
                     /translation="MKTLKHFPFAQIAHKTIACLLTFCMVNLPVWALDAGDASVSAGT
                     AVIHSPAANTVSVDLITPRAVVDWTQMNAAGTETLNFAGASGFAVLNRVQSAVHFDGS
                     LNGIGGHVFVVSPNGVVIGPNAVITASAFTAAGLNISNANFMSGVYAFVPFTGGVTGD
                     VVNQGRIHVTEQAALLGRMVTNSGTIVSPSGLVLMAAGESVFLSTSGSNVIVEVDGVN
                     AAAAGAGDVINSGTIQADEGQIILAAGDIYSSALGIRPEEGIGRVIQSGHMHADGTAA
                     GSGSGGSILLTASDEVLLAAGSSTTANAIPDGIGPDRPDGGSIVLSSANTVRMDTDAF
                     VKAKGNGWYDIDNNLRYDLGTDPDFRGSIQVAGRYIVLPSPDDYASAVAFRNHIDLMP
                     WSGNPEQVGLLTAGPLTGDLHIANGDAAGMPAANTIYEEWIEDVSKAGINVDLLSPAD
                     ILIADLNPGEPKDLGLVGGSGDLSFRTMFQDGGISLADIADLIATSDGGNLFMLAGSG
                     GISVGDLKTYTSSSDKVTDSGQIFLLTSWPQGAETGADISTGSITVNGGGNSEISVIA
                     TGSLTVNGDVISTRNSVPQQDPSVRKALVCLIAGENVTVRGDIASTSHGKYATTAAIR
                     VSGGWDVVIGNSSSITAEAKTSEQGPYKADASVVLQAGWNKNNADGIITLNGIAYNDQ
                     PGGSNSAYASLIKVSASVSGIGQNASVKTSDSWSENDPLPAASDDGSHKFWKDEKSKG
                     SATAAASILIDN"
     gene            complement(2298..4151)
                     /locus_tag="PLE88_10020"
     CDS             complement(2298..4151)
                     /locus_tag="PLE88_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOM76840.1"
                     /translation="MTPSNKKKNLQIIALVVLAAFISLPVLSVRLPEPQTEQQQLLEQ
                     KAAAEETIKTAEDIRPADSSAAGLPEDTTPRFTIRRIVFSGNTLLTDKQILAAIPAVY
                     AADGQTLYDLRPLQALAAHPGTEQTVSARSIQGFTQYILSVYQKKQYAGIYVYIPAAA
                     FASGGQLSEGILPIEIIEAAVSSVSSAYYNPSNQPAQKRHLSEERLLGWSPVKEGGAL
                     SRKQLDDYLNLLNLNPDRYVAALVSKGAQTNSLAVKYNVYEANPWHYFIQLDNSGTKD
                     IQWRPRIGVINTSLLGYDDKFTAVYQTTPDSTWDDEYAVYGSYDFPLMGPRLRLNLFA
                     GYNEFSITDPDTNFLGAGKFAGGILRYNALQFNDWFFDITGTLSYEESRITPEFFPEF
                     FETDIRMVLWGCGADLYKTTDTADTFLGVSMLSPLDTSELQEIRTARTNAVDNFHIYY
                     VSGRHSRYLDSGKIQRVSASLRYITSDDRLVPAKMTSFGGMYTVRGYDEYEIVADGGI
                     IGSFQYEYDLVRKSQVELFGQEVNEKSRKPFLKKLAPLGFFDYGQARIKDAVSAAAET
                     TDQELASLGCGLIAELGSHFTGTVYYGYPLIPTDDTRSGKGRVHIGLLLRW"
     gene            4312..4575
                     /locus_tag="PLE88_10025"
     CDS             4312..4575
                     /locus_tag="PLE88_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76841.1"
                     /translation="MHTKTDPNAGITKNYRTYLRDSAGVVLKFNRQMAYGKKKKSGIY
                     PAGYTQRAAASSAQNPADYLQLFVRVECLKNQKLPSEFLWKQL"
     gene            complement(4843..7470)
                     /locus_tag="PLE88_10030"
     CDS             complement(4843..7470)
                     /locus_tag="PLE88_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM76842.1"
                     /translation="MKWAAKLTVLVVFMGVMNCSYLSASKAIDQDREYIRWTPLPDLP
                     ESLGVAGPFVGVHHDVLLVAGGANFTPPVWESEKQWLDKIWALEKIPSSEEKTAKKYQ
                     WIDAGRLDVPVAYGAAVSTEQGVVCIGGCNSEQVFRNVFALAWDPQKRTVCRKDLPSL
                     PKPCVYTYAAKIGSVIYVAGGQSGISLDTAMTNFWSLDLSKEGTEAFAWKEWTPWPGP
                     SRAFNITVAQHNGMTDCIYVISGRRLNPGADAIETEFLQDVYEYNPLKVNSKEGPWRK
                     RCDVPACVMAGAGIDIGQSHIFILSGADSSLFFKNTQLKDDHPGFPLQAWAYHTITDT
                     WIDAGRTPANQVTTIPVKWGNSIILASGEIKPRVRSPKIWEITPVTIKNSFGIINFTT
                     LGIYLFLMIAVGFYFSARNKNTDDFFRGGQRIPGWAAGLSIFATMLSSITFIAIPAKV
                     YATDWTFFIVNMMAVAVAPFVMWYILPFYRRIDATSAYEYLEKRFHVAARLFAGLSFV
                     LFQTGRMAIVIFLPSLALSAITNVSVPYCILIMGVVSIIYCTLGGIEAVIWTDAVQSF
                     VLLGGAFMSLAVIILGIQREGGEFFAAACSHNKFHMINWDFSTASYMTASLWVMVIGG
                     IGQSLVPYVSDQAVVQRYLSVRDIKTARTSILTNAIIVIPATILFFGIGTALFVFYRV
                     NPENLDPTYQNDAIFPLFIARQLPVGISGLVVAGIFAAAQSTISTSMNSISTVIVTDF
                     VKRFSLLNTEKGYLNVARFCTVFFGILGTGLSLLLASADIKSLWESFMEVLGLFGGSM
                     CGLFLLGIFTRRVGTAAALAGAAFSVVILFLIQGKTHTSVLIYASIGIAVCMLSGYLL
                     SFVFPQKQKDLAGLTIYTV"
     gene            complement(7664..9862)
                     /locus_tag="PLE88_10035"
     CDS             complement(7664..9862)
                     /locus_tag="PLE88_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOM76843.1"
                     /translation="MNILEKKIDILGIFIFLLAAAFSSAELFSVHPDDSTGTVRVACV
                     GDSITCGLKIENPQENSYPAQLQKLLGDRYEVQNFGCSGARVCKATRVPYLNQPECRN
                     AVSFKPHIIIVCLGINDASVPEWHNNKPNFVRDYLELIQHFTNVPKIQTPKIWLTTLL
                     PVMPPYEPYVEIQQTKTEADAMIRQVADLLNLPVIDLFSPLISEPQFFPDGLHPDING
                     AAIIAQTVAKAITGDYGGLKLPWVFGDHMVLQRHMPIPIFGTANVGSTVSVKLGNTTI
                     QTTTDANGRWRIDLPAMEAGGPYELVCSDRSKTLRFTDILVGEVWYAAGQSNMDWPCQ
                     LDADWKNEAPSADRFPQIRLLNRQGYPSTGPIRWTQEQIDRVTIDAYYSGCWQVGSQE
                     WAASISAVAYYFAREIYQAIGVPVGIIEAAVGGASMEAFIPPEALAAESLYPLTKQWI
                     EASDAPDWHRKRARQNLGIWFDGPRQTPMPHHPFEPSFLFNADIADLIPFAIRGVIWY
                     QGETNATDASNQIAWNPEQNRLLFKTLIQSWRRRWGQGDFPFYYVQLPNLNRPWMLFR
                     DMQCRMLNELPNLGMAVTIDLGHPTNVHPPAKREVARRLSLWAQTHLYGRPMAVCSGP
                     LHNGFIQKDAHTLKVGFDPATGPLCSRDGDTLRGFEVADSAGRWYSANATIEDHQVIL
                     FSPEVSNPVAVRYAWAPNPQATLYNTAGLPASPFWSGLMVCERPEKFLSQ"
BASE COUNT         2710 a         2722 c         2575 g         2546 t
ORIGIN      
        1 attatcaatc aaaatgctgg ctgccgctgt tgcgcttccc tttgattttt catccttcca
       61 gaacttgtgc gagccgtcat ctgaagccgc tggaagcgga tcgttctcgc tccaggaatc
      121 cgatgtttta acactggcat tttgtcctat gccggaaaca gaggcactga ctttaatcag
      181 tgaggcataa gcgctgttgc ttccgccggg ctggtcatta taggcaatgc cgttaagggt
      241 aatgattccg tcagcgttgt tcttattcca tcctgcctga agaacaaccg aagcatccgc
      301 tttgtaaggt ccttgctcag aggttttggc ttccgccgta atagaggaag aattgccgat
      361 aacaacatcc catcctccgg aaacacgaat ggctgccgtt gtcgcatatt tcccgtggga
      421 agtagaagca atatctcccc tgacggttac attttctccg gctatcaggc aaaccagcgc
      481 cttgcggacg ctgggatcct gctgcggaac actgttgcgt gtggagatga catctccatt
      541 gaccgtcagc gagccggtgg cgatgacgct gatttctgaa ttgccgccgc cgttaaccgt
      601 aatcgagccg gtggaaatat ccgctccggt ctccgctccc tgaggccaag aagtcagcaa
      661 aaagatttga ccggaatctg tcactttatc gctgctggat gtataggttt tcaaatcccc
      721 taccgaaatg cctccgcttc cggccagcat aaatagattg ccgccgtcgg aagtggcaat
      781 caggtcggca atgtcggcaa gggaaatgcc gccgtcctga aacattgttc gaaatgacaa
      841 atcgccgctt ccgccgacca agccgaggtc tttcggctca ccgggattca aatcggcgat
      901 tagaatgtca gcaggcgaca gcagatccac attgatgcct gcctttgaga catcttcgat
      961 ccattcttcg taaatcgtat ttgctgccgg cattcctgca gcatcgccgt tggcaatatg
     1021 caggtctccg gtcagcggtc ctgctgtcag cagtccaacc tgctccggat tgccggacca
     1081 cggcatcaga tcaatgtggt tgcgaaatgc gacagcggat gcataatcgt ctggcgaagg
     1141 cagaacgata tagcggccgg ccacctgaat agaaccgcgg aaatcagggt ctgtacccaa
     1201 gtcataacga agattgttat cgatatcata ccagccgttg cctttggctt tgacaaacgc
     1261 atcggtatcc atccgaacgg tattggccga agacaagacg atgctgccgc catccggacg
     1321 atcgggaccg atgccgtctg gaatggcatt ggcagtcgtg gaactgccgg cggccaaaag
     1381 cacttcatcg ctggcggtca gcaggatgct tccgccgctg ccggagccgg cggcggtgcc
     1441 gtcggcatgc atgtgaccgc tctggatgac tcggccgatg ccttcctcag gccgtatgcc
     1501 cagtgcggat gaataaatgt ctccagcggc cagaataatc tgcccttcat ccgcctgaat
     1561 ggttccggaa ttgatgacat cgccggcccc ggcggctgcg gcattcacgc cgtcaacctc
     1621 aacaattaca ttgcttccgg aggtggagag aaaaacgctt tccccggcgg ccatcagaac
     1681 cagcccggat gggcttacaa ttgttccgct atttgttacc atgcggccca gaagagcagc
     1741 ctgctcggta acatgaattc tgccctgatt caccacatcg ccggttacgc ctcctgtgaa
     1801 gggtacgaac gcataaacgc cgctcatgaa gttggcattg gagatgttca ggccggctgc
     1861 tgtgaaggcc gatgccgtaa tgaccgcatt ggggccaata acaaccccat tggggctgac
     1921 gacaaaaaca tgtccgccga tgccgtttag ggagccgtca aaatgcacgg ccgactggac
     1981 ccggtttaac acggcaaatc ctgaagcacc ggcaaaattg agtgtctcgg tgccggcggc
     2041 attcatctgg gtccaatcca ccacggcacg cggggtaatc agatccacag aaaccgtatt
     2101 ggccgccgga ctgtgaataa cagccgttcc ggcagatacc gaggcatccc cggcatccaa
     2161 cgcccatacg ggcagattga ccatgcagaa tgtcaacagg caggcaatgg tcttatgcgc
     2221 gatctgcgca aacggaaaat gttttagagt tttcatttgt ttcccctcaa caatttttta
     2281 tcagcagttt gcgatttcta ccaccgaagc agcagaccta tatgcacccg gcctttgccg
     2341 cttcgtgtat cgtcagtggg tatcagtgga tacccataat agactgtacc ggtaaaatgg
     2401 ctgcccagtt cagcaatcag gccgcagccc agcgaagcca attcctgatc ggttgtttca
     2461 gcagctgctg aaacggcatc tttgatgcgg gcctgtccgt agtcaaaaaa gcccaaggga
     2521 gccagtttct tcaggaaagg cttgcggctt ttttcgttta cttcctgacc gaacagctct
     2581 acttggcttt tccgcaccaa atcgtattca tactggaagg aaccaataat gcctccgtcg
     2641 gcaacgattt catattcatc atatccccgt acggtataca ttccgccgaa cgatgtcatt
     2701 ttggcaggca caaggcggtc atcggaggta atatagcgca gcgaggcaga tacccgctgg
     2761 atcttgccgg agtccagata ccggctgtgg cggccgctga cataataaat gtgaaaattg
     2821 tccacggcgt tggtgcgggc tgttcgaatc tcctgcagct cggatgtatc cagcggactg
     2881 agcatactga ctcccaagaa ggtgtcagct gtatcggttg ttttatacag gtcagctccg
     2941 cagccccaca gcaccatgcg aatatccgtt tcaaagaact ccgggaagaa ttcgggagtg
     3001 attctgcttt cttcgtagct gagtgtgccc gtaatatcaa agaaccagtc attaaactgc
     3061 agggcattgt agcgcaggat gccgccggcg aatttcccgg cgcccagaaa attcgtatcc
     3121 ggatccgtaa tgctgaactc gttgtagccg gcaaacagat tcagccgaag tcgggggccc
     3181 ataaggggaa aatcgtagct gccgtaaacg gcatactcgt catcccaggt tgaatccggc
     3241 gttgtctgat agacggctgt aaacttgtca tcatatccca gaaggctggt attgataaca
     3301 ccgatgcgag gccgccactg gatatccttt gtgccggagt tatcgagctg tatgaaataa
     3361 tgccacggat tggcctcata cacattgtac ttcacggcca aactgttggt ctgggctcct
     3421 tttgagacca gcgctgcaac atagcggtcg ggattcaaat tcagcaggtt gagataatca
     3481 tccagctgct tgcggctgag cgcgccgcct tccttcaccg gcgaccagcc cagcagtctc
     3541 tcttcgctca gatgccgttt ttgcgcgggc tgattgctcg gattataata ggcagacgaa
     3601 acagaagata cggcagcctc gataatctca atgggcagaa tcccttcgga cagctgtccg
     3661 ccggacgcaa aggcagccgc agggatataa acataaatgc ctgcatactg tttcttctga
     3721 tagaccgaca gaatatattg cgtaaagccc tgaatgctcc gggccgagac cgtctgctct
     3781 gtgccggggt gagctgcaag tgcctgcaag ggacgcaaat cgtaaagcgt ctggccgtca
     3841 gccgcataaa cagcggggat agcggccaga atctgtttgt ctgtcagcag ggtgttgccg
     3901 ctgaatacaa tccgccggat ggtaaagcgg ggggttgtgt cctcaggcaa tcctgccgcc
     3961 gaactgtcgg caggccgaat gtcctccgcc gttttgattg tttcttcagc agcggccttt
     4021 tgttccagca gctgctgctg ttctgtctgc ggctcgggca gccggactga aagtacgggc
     4081 agactgataa atgctgccag caccaccaaa gcgattatct gcaaattctt ctttttgttc
     4141 gatggtgtca ttggacctcc ttgaaattat tgcactgcct tgttaaggcg ttgtacaaat
     4201 tgcggctccc aattgtgata ggtctgtgct attatgtcag ttatgcttat tatacttatt
     4261 tttgattatt tagtcaatcc cggaagcagc ttgctgtgtg caattgccta aatgcataca
     4321 aaaacagacc ctaatgcggg aattaccaag aattatcgga cttatcttcg ggactccgca
     4381 ggagtcgtcc tgaaatttaa taggcagatg gcgtatggca aaaagaaaaa aagcggtatt
     4441 tatcccgctg gttataccca gcgggcagca gcttcatcag cacaaaatcc cgctgactat
     4501 ctccagctgt ttgtgcgggt tgaatgttta aagaatcaga agctgccgtc cgaattccta
     4561 tggaagcagc tgtagttgta ccgccggcac gtccaatgca gggattattc cttcatgcct
     4621 ataagaccat gtacgtcagg aggttcgtcc gcagacccgg ttccggccgg cttagccggc
     4681 gggtatggat gaatcagaaa accagttgta gatttaagcg gtgaaaatat cactgttttt
     4741 cataggtaac ctctgggctg ataagagaat cggcaaagat aaaaaagtga gttgcagtaa
     4801 ttttgaaaga cgtgttaacc ggaaagtcaa tatgaagcgc tgtcaaacag tataaatggt
     4861 caatcccgcg agatctttct gcttttgggg aaaaacaaat gacagcagat agccgctcag
     4921 catacagaca gcgataccta tagacgcata tattaagaca gatgtatggg ttttcccttg
     4981 gatcagaaac aaaattacta cgctgaaggc tgcacctgca agggccgctg cggtgccgac
     5041 gcggcgagtg aaaattccca gtagaaacaa tccgcacatg gaaccaccga aaagccccag
     5101 aacctccata aaagattccc aaagagattt gatgtcggcg ctggctaaca gcaatgacag
     5161 acccgtcccc aagattccaa agaagaccgt gcagaaacgg gccacattca gatacccttt
     5221 ctcagtattt aacagggaaa aacgttttac aaaatctgtt acaatcaccg tcgaaatgct
     5281 gttcatgctg gttgaaatcg ttgattgtgc cgctgcaaag attcccgcca ctaccagccc
     5341 cgaaattccg accggcagct gacgggcgat aaaaagcggg aagattgcat cattctgata
     5401 ggtcggatcc agattttcag gattaaccct gtaaaaaaca aacagggctg ttccgattcc
     5461 gaaaaacaaa attgtagccg gaataacaat gattgcattg gtcaaaatgg atgttctagc
     5521 ggtttttatg tcacgaacag ataaataccg ttgcacaacc gcttggtctg aaacataggg
     5581 aaccagtgac tggccgatcc cgccgatgac catcacccac agagatgcag tcatataact
     5641 ggctgtgctg aaatcccagt taatcatatg gaacttgtta tgactgcaag cggcggcgaa
     5701 aaattctcct ccctctcgct ggatgcccaa gatgataacg gccaaactca taaaggctcc
     5761 tcccaataaa acaaagctct gaacggcatc cgtccagatc accgcttcta tgccccccag
     5821 tgtacagtaa ataatgctca caactcccat aattagaatg caatagggga cgcttacatt
     5881 ggtgattgcc gaaagggcca aagatggcaa aaatatgacg atcgccattc ggcctgtctg
     5941 aaacagcaca aatgacaaac cggcaaacag gcgggcagct acatgaaacc ttttttcaag
     6001 atattcataa gcactcgtgg catcaatgcg tctgtaaaac ggaaggatat accacattac
     6061 aaaaggagca actgcaacgg ccatcatatt gacaataaag aaggtccagt ccgtcgcata
     6121 gactttggcc gggatggcga taaaagtaat cgaactgagc attgtagcaa atatacttaa
     6181 tcccgctgcc catccgggaa tccgctgtcc gcctcggaaa aagtcatcgg tatttttatt
     6241 ccgcgcggaa aaataaaaac cgacagctat catgagaaat aaataaattc ccaaggttgt
     6301 gaaattaata atcccaaagg aattttttat tgtaacggga gtaatttccc agattttggg
     6361 ggaacgcacg cggggtttta tttctccgct ggcaagaata atggagttac cccatttgac
     6421 gggaatagtc gttacttgat tggcaggagt ccggcctgcg tctatccaag tatcggtaat
     6481 tgtatggtag gcccacgctt gcaggggaaa tccgggatgg tcatccttca gttgtgtgtt
     6541 cttgaagaac agggaactat ctgcaccgct caatataaag atatggctct gcccaatatc
     6601 gattcctgcg cctgccatca cacaggcagg gacatcacac cgcttgcgcc acggtccttc
     6661 cttactgtta actttcagtg gattgtattc atacacatcc tgaagaaatt cggtttctat
     6721 tgcgtcagct cccggattaa gccgtctgcc gctgattacg taaatacaat ctgtcatgcc
     6781 gttatgctgg gccacggtga tgttgaatgc ccgagatggt ccgggccagg gcgtccattc
     6841 tttccaagcg aaagcctccg tgccttcctt tgatagatca agggaccaga agttggtcat
     6901 cgcagtatcc agagagattc ccgattggcc tccggccaca taaatgacgg aaccgatttt
     6961 ggcagcgtac gtatacacac agggttttgg caaggaggga aggtcttttc tgcatacagt
     7021 tcttttttga ggatcccatg caagggcaaa aacgttccgg aacacctgtt cggaattaca
     7081 gccgccaatg caaacaactc cctgttcggt agagacagcc gctccgtaag caaccggaac
     7141 atccagcctt ccggcatcta tccattggta tttttttgct gtcttctcct cggaagacgg
     7201 tatcttttcc aatgcccata ttttatcgag ccactgcttt tcgctttccc atacaggcgg
     7261 agtgaaattt gcaccccccg caaccagcaa tacgtcatgg tggacgccca caaaaggacc
     7321 ggctacaccc aaagattccg gcaggtccgg aagaggagtc caccttatat attccctatc
     7381 ttggtctatt gctttggagg cagaaagata gctgcaattc attaccccca taaacacaac
     7441 caacacggtc aatttcgctg cccacttcat tgtatctcct tttgatcagt atttatctca
     7501 tagtgttttt gttctgcaat gcggcaagtc ctaagtgtac aagtgcgtac actttcgacc
     7561 ctcatcctgc tgtcccgcat tgccggtgtc gcctagccga tcctgtaatg tactggtaat
     7621 caatggtgac cagcagcggc tattcggcag attgtgtata ggtttattgc gataagaact
     7681 tttctgggcg ttcgcatacc atcaatccag accagaaagg cgaagccggt aaacccgcag
     7741 tattatacaa ggttgcctgc ggattgggag cccatgcata acgcactgca acaggatttg
     7801 atacctcggg gctgaacaga attacctgat gatcctcgat ggttgcgtta gccgaatacc
     7861 atcggcccgc agaatctgca acctcaaatc cccttagcgt atccccatcc cgggaacaca
     7921 acggtcctgt ggccggatca aaaccaactt ttaatgtatg agcatctttt tgaataaaac
     7981 cattgtgcaa ggggccggaa cagactgcca tcggacgccc gtagagatgc gtttgcgccc
     8041 atagactcag ccgccgggcc acctcccgtt tggccggcgg atgcacattt gtgggatgcc
     8101 ccaagtcaat tgtcacagcc atccccaaat taggcagctc gtttaacatt cgacactgca
     8161 tatcccggaa cagcatccac ggtcggttca gattcggcag ctgcacataa taaaaaggaa
     8221 aatctccctg tccccacctg cggcgccagc tttggatcaa cgtcttgaaa agcagacggt
     8281 tttgctccgg attccaggcg atttgattcg atgcatcggt tgcattggtt tcaccctggt
     8341 accagataac ccctcgaata gcaaagggga ttaaatcggc gatgtcggca ttgaataaaa
     8401 aactgggttc aaagggatga tgcggcatcg gagtctggcg cggaccgtca aaccagatgc
     8461 ctaggttttg gcgtgcccgc ttacgatgcc agtctggtgc atcggacgcc tcgatccact
     8521 gtttggtcag aggataaagg gactcggctg ccagtgcctc cggcggtata aatgcctcca
     8581 tcgaagcccc tccgacagcg gcttcaatta ttcctaccgg aacgccgata gcctgataaa
     8641 tctcccgggc gaagtaatac gccactgccg agatggaagc tgcccattct tgcgatccaa
     8701 cctgccagca gccgctgtaa taagcgtcaa tcgttacccg atcgatttgt tcctgcgtcc
     8761 acctgatagg gccggtggac ggataacctt gccgatttag caaacgaatc tggggaaagc
     8821 ggtcagctga cggcgcctcg tttttccagt ctgcatccag ctgacagggc cagtccatat
     8881 tggattgtcc ggcagcatac cagacttccc cgaccagtat gtctgtaaat ctcaatgtct
     8941 tggacctatc cgaacagact aattcataag ggcctccggc ctccattgca ggcagatcta
     9001 tgcgccatcg cccattggca tccgttgttg tctggattgt ggtattgccc agctttacag
     9061 agacagtcga accaacattg gcagtgccga aaatcggtat cggcatatgg cgctgcaaaa
     9121 ccatatgatc cccgaacacc catggcagtt tcagaccgcc gtaatcgccg gtaatggcct
     9181 tggctacagt ttgagcgata atagccgctc cgtttatatc ggggtgcagt ccatcgggaa
     9241 aaaactgcgg ctcagaaatc agcggactga ataagtcaat tactggaagg ttcagtagat
     9301 cagccacctg ccgaatcatt gcatcagctt cggtttttgt ttgctggatc tctacataag
     9361 gttcataagg cggcatcacc ggaagcagcg tggtcagcca gattttgggc gtttggattt
     9421 ttggtacatt ggtaaaatgc tggattaact ccagataatc ccggacaaaa ttaggcttgt
     9481 tgttgtgcca ttccggcacc gaggcatcat taattcccag acaaactatg attatgtgag
     9541 gcttaaaaga aacagcattc cggcattccg gctgattcaa atagggtacg cgggtagctt
     9601 tgcagactcg cgccccggag cagccgaaat tttggacctc gtatcggtct ccaagaagtt
     9661 tctgaagttg tgccgggtat gaattttcct gcggattttc aatcttgaga ccacaggtaa
     9721 tactgtcacc cacgcacgcc accctgaccg tcccggtgct atcatcagga tgaacagaaa
     9781 aaagttcggc cgagctaaag gccgcagcaa gcaaaaaaat aaaaatccct aaaatgtcga
     9841 ttttcttttc caaaatattc attgtttctc taatcatgct tctgggtgtt tttcacatcc
     9901 acaagcaatc ctttgccccg acccgaatag cctgctccat ctgtggataa aaagcagccc
     9961 ttagtaacat taaaaccatc tgtaaaatat gccggacaac aatggccgca gtcggcagaa
    10021 ggttaatgtc cagcatgtta ggaatactta taaacttgca gtatttaaaa gcgttgatgg
    10081 tgcaacattg ggaagtgtat ctggtagaga acacgctgga atgtatagca tccatttaaa
    10141 agttcctgct gagacaaaag ctttgcttcc cttcaggccg tcattataat ccgatgcatc
    10201 agatctcaaa acgccatttc ataacatcga ctgtatttat cgtatgattt gaagcaacag
    10261 ctacggtatt ccgcatcttc aaatcaatat gagaaattat agtctttccg ataacggtat
    10321 tcttaagaca agaacatcag gcaataccac gactggtcca ataaaaagcc gacaacctgc
    10381 tgtgcaggac agtcacaaaa cagtttatat gctgtagccc cgtcactctc agctggaagg
    10441 cagttgcagg ggaatcgatg ccagcgcgag gatttcagca taagctaacg ccctgtcgta
    10501 gattcgtacc tcatcgagaa ggccttcaaa gaaaactgtt ccaatcagat taa
//
LOCUS       DAOTBO010000093        10348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5480_length_10348_cov_6.824832, whole genome shotgun sequence.
ACCESSION   DAOTBO010000093 DAOTBO010000000
VERSION     DAOTBO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_10348_cov_6.824832"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLE88_10040"
     CDS             complement(<1..259)
                     /locus_tag="PLE88_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76844.1"
                     /translation="MHGTFQLWHFNDNQSLYITYNPDDIRYINLQRWRWIMPESEYRR
                     MTHRECLMFMSEHWLDQTFGFRLEDFAALSRHWSGHTPYQEE"
     gene            complement(361..603)
                     /locus_tag="PLE88_10045"
     CDS             complement(361..603)
                     /locus_tag="PLE88_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76845.1"
                     /translation="MLTPEEEAKIELIVTRAITAYSQQVEKMIESRISVHEKTCEAAE
                     QFRIMKAKFIGLLVGVCLSSAGVGGAVSAIMSVFMR"
     gene            complement(624..1373)
                     /locus_tag="PLE88_10050"
     CDS             complement(624..1373)
                     /locus_tag="PLE88_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76846.1"
                     /translation="MATKSIWKSKNLIIYDDRYPSILSSLWKDCPLLAWYNDPSIGTY
                     LFEDFNNYHAATLAGYTATQATSGTFTLGDEEYGTAVLNAGASTQHQGINVQKKGLMV
                     KPAAGKTIWFECRFKAANPTKLQGFIGLASTDTTLMPSGVMDSSNSEYIGAGSPTTAA
                     GVAKLYGCKATTEGTVNSIFTFGTSYITFAMKIDGISNVYYSVDNVVGSNTLGTSYIP
                     TNALTPSFICQSDGVSQPTLTIDYYRVFQLR"
     gene            complement(1444..1911)
                     /locus_tag="PLE88_10055"
     CDS             complement(1444..1911)
                     /locus_tag="PLE88_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76847.1"
                     /translation="MVQDKIKFVEAIPPANYSGAAGTGNYISLKNYRKCAIVINTGAW
                     AGGTAAVTVNKATEVSGLGATAASFSYMYTNDGAATGSLLTKTAVTSNTFNLDTANSM
                     YVIEIDAASLGDCDCIQLAVASPGANNDYYSATYVLSEPRFKDDYDTVEPLTD"
     gene            complement(1934..3298)
                     /locus_tag="PLE88_10060"
     CDS             complement(1934..3298)
                     /locus_tag="PLE88_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOM76848.1"
                     /translation="MLVRLIKEWNSGAKNYPVGQLLEIPAEKAKSLIENAVAEIYEPQ
                     QSDVVAESTVKNEAQYSLTEDLLRLINEKIKEQIQNYARKNSSFHQASESSDYEKTGG
                     FKSIGHLAHEVYRASVSRSMPETLGKWTNYLNTKASGMQESVGSDGGYLVPTAYRSEL
                     MRVAVENSVLLGRVTRIPMETNSVKIPTIDETSRAASVYGGIVVYRPTEGGTITGSKP
                     KIGSVQLNLNKLAAMAYVSSELLEDSPISIEPLFATTFGEAIGFQIDEDIINGTGVGQ
                     PLGILNSPSLISVAKETGQAAATIVAENILKMWSRMLPACQKNAIWIANNDTFEQLAS
                     LSLPVGTGGIPAGLLQMSTNGLTGTPQQTLINKPLFLTEHAQTLGTVGDIICIDPTQY
                     LFGEKAGGAIQAATSIHIKFVEDEVAFRFTLRYDGQPWMKSAITPKHGSNTLSAFVAL
                     ATRS"
     assembly_gap    3545..3644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3645..>5606)
                     /locus_tag="PLE88_10065"
     CDS             complement(<3645..>5606)
                     /locus_tag="PLE88_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOM76849.1"
                     /translation="FKPADARPAVYTIGNEPFYWRSSGRPEDLIQKVKNWVYVCIDRN
                     AAACAQTPLRLYQTKKQNKYKSRPADRNAVQRIVSKVYLDAVSDIEELVEHPLLDILK
                     RPNPVNNLYELLNITVSYLEAIGNAFWYLETENNQVLNIWPLLSQFVSIRKIDNEIYY
                     KYQHGGKQMEFTAEQIIHFKYPSLTDPYLGTSPLQACEQAADLYDFMNRSEISLMKNG
                     GIPDAVIQFPPDAFISEEEERRILTKYKQFQGPDRRGKLSILTGGAEIKPVGFAPKDM
                     NYLQGRKSAVEEICGVFGVPLSFVKIQEISRANAWASLDLWAQQVIRPKLVVLENKLN
                     ERLVPLFDDSLLLMFDNPRPPDEEFRLRQIETRLRVNYTSINEERAIDGFPPVEWGET
                     PVNPLSQTIQAETPTAEETSEKSYRPAEPPQYKENAILITHLVLFYKEMISEIGRKLK
                     KLEEKAVVNDVISTVFDNKHWTKRLEETLSPFLRGIMINRMIEEMSKIKPDGVINASS
                     PEVRRALEKRKGKIATLVSNSERKIRKLIEQGINEGMGAAKIADLIKDNFNTLADAER
                     VARSETIWAHNEGIEQAWKQSGVVAYKVWDSSGDDRSCPFCRSMHGKKVSLDSDFIQK
                     GDTLTVKDTDIELDYENIAHPPLHPNCRCA"
     assembly_gap    5607..5706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5707..6677)
                     /locus_tag="PLE88_10070"
     CDS             complement(<5707..6677)
                     /locus_tag="PLE88_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HOM76850.1"
                     /translation="MILIQTSGLCKFINELYFKLINNRSRYLIYYGGAGSGKSVAAAQ
                     KQIIRIIQAFYTKKPVRLLCMRKTTPAVRKSAYEEIKSLLYKWSLSQIVTENKTRLQL
                     VFPDSSEILFMGLDDPEKLKSIHGITSVWMEEATEFTLDDFRQIDLRLRGKSNQYKQI
                     LMTFNPINEQHWIKKQFFDDDIQKEIESGKQIVSRKYTANSDITIVHSTYKDNRFIDH
                     AYIERLEHLIEEDQNYYNIYTLGKWGVLSGRIFENYSTAETVPDKYDIRYYGLDFGYS
                     IDPAAFVECRIIGKTLYVKEHLYQPNLTNKELADLIRSILDANQDKNG"
     gene            complement(6674..7570)
                     /locus_tag="PLE88_10075"
     CDS             complement(6674..7570)
                     /locus_tag="PLE88_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76851.1"
                     /translation="MNKSMIDNIYNPNDFCIFIISYQRANNVKTYKTLQKCNCKYPIF
                     IVVSDDDTQINQYKKNFSENNLVIYSKREYIGKFDYMDNRIDKLSSAVFPRNAVFDIA
                     KKLGYPFFLVLDDDYGNFEYRYMQNNKLRGKILHKSLDKILYLYKETLKALPNIHSLA
                     MSQAGDFIGGNMTRAFSYRRLRKIMNVFFCATDRRFEFFGRLNEDVNVYTKLGNIGYL
                     FLTIPEISIVQLITQHNEGGLTSSYLELGTYIKTFYTLLAMPSSVKIGIIQDTFSRIH
                     HQINWKFTIPYILPDEVKKYKK"
     gene            complement(7563..8351)
                     /locus_tag="PLE88_10080"
     CDS             complement(7563..8351)
                     /locus_tag="PLE88_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOM76852.1"
                     /translation="MKVILYEIDDIKPYKDNPRKNENAVDVVAESIKKYGFRQPIVID
                     KDGVIIAGHTRWLAARKLGLKKVPVHIASDLTPEQTRAYRLIDNRTADFAEWDIEKLK
                     TELDALIDNKELNFLLKNCDFDKLLQSESIEKQDITKETIYSQKIEVPVYKPMQENPP
                     AINELYDITIYKQKLKQIEETGIKDKKLIEFLQLAATRFIKFNFQNIAEYYAHQPAEV
                     QDIMEKLVLIIIDIKNAIANGMVVIHKKIKDIMADEILSEDLDE"
     gene            complement(8354..8794)
                     /locus_tag="PLE88_10085"
     CDS             complement(8354..8794)
                     /locus_tag="PLE88_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76853.1"
                     /translation="MGRKSNYKPDYVKLAYNACSRNGADIAALANLFGVHVDTIYDWM
                     HKQPDFKEAICSGRDLFDTEKVERKLVELALGYEYEDIVAEKDEKTGRVRVKKYKKQL
                     PPNPAAIIFYLKNRQPGRWKDKIDNQVSSDLKIVVDLPDDLKPD"
     gene            complement(9112..9477)
                     /locus_tag="PLE88_10090"
     CDS             complement(9112..9477)
                     /locus_tag="PLE88_10090"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HOM76854.1"
                     /translation="MLHYNIPIRPIPQQRPRVYRRDGRIYAVNPSQKIKNEIAKFIYP
                     RPAQLLCGPIEVKIEFYYKGKTSGYCPKYADIDNLAKLVLDALNGIIWEDDKQIVKLL
                     AEKKYAEKEEIRIFAKIID"
     gene            complement(9534..9683)
                     /locus_tag="PLE88_10095"
     CDS             complement(9534..9683)
                     /locus_tag="PLE88_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76855.1"
                     /translation="MKSKLQIIEERMRRLEILYKILAAQKKVERIKQQLDNRRDANVK
                     QSFTD"
     gene            complement(9824..10072)
                     /locus_tag="PLE88_10100"
     CDS             complement(9824..10072)
                     /locus_tag="PLE88_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76856.1"
                     /translation="MISQEEKENLKVYRMENERKIDNLIHLRAAARRFGLPPKWLKEQ
                     AISGAIPALIADNKVLFDLEVLVQWLTKRARGTGDDGK"
     gene            complement(10077..10307)
                     /locus_tag="PLE88_10105"
     CDS             complement(10077..10307)
                     /locus_tag="PLE88_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76857.1"
                     /translation="MTKFVNISNHPIAGWTDKQKQAAKDLFPAGSKVKFVDLGFPNID
                     PKKSTEKVFVDYSSRYIDKYIEAEEQLLPIKE"
BASE COUNT         2560 a         2332 c         1982 g         3274 t
ORIGIN      
        1 gttcttcctg atacggtgta tgccccgacc aatgccggct taaggcagca aaatcttcca
       61 accgaaagcc gaaggtttgg tccaaccagt gttcggacat aaacattaag cattcccgat
      121 gtgtcatccg ccggtattcc gattccggca taatccatcg ccatctttgc agattgatat
      181 agcggatatc atccggattg taggttatat ataaggactg attatcgttg aagtgccaaa
      241 gctggaatgt cccgtgcatc ggaacgtctg ctcttgccca ataaaataac agcaggcagg
      301 tagcaaacat aacctgcctg ctgccggagg aaagggaaag agaatgagaa gaataagtct
      361 tcatctcata aataccgaca taatagccga tacggcgccg ccgacaccag cagatgataa
      421 acatacgccg accagcaatc ctataaattt tgctttcatt atacgaaact gctcggccgc
      481 ttcgcaagtc ttctcgtgaa ctgaaattct ggattctatc attttttcga cctgctggct
      541 gtatgccgta attgcacgag tgacaatcag ttcgattttt gcttcttctt ctggcgtcaa
      601 cattaattgt ccctttgcgt taatcatctc agttgaaaga ctcggtaata gtcaatggtc
      661 aatgtcggct ggcttactcc gtctgattgg cagataaaag acggtgtcaa agcgttggtc
      721 ggtatataac ttgtccccag cgtgtttgaa ccaaccacat tatcgaccga gtaataaaca
      781 ttggatatgc catcaatctt catcgcaaat gttatgtatg acgtcccgaa ggtgaatatc
      841 gaattcactg tgccttcggt cgttgccttg cagccatata atttcgccac acctgcagca
      901 gttgtaggtg aacctgcccc gatgtattcg gaattcgagc tgtccatcac accggacggc
      961 atcaaggtcg tatcggtcga agccaatccg ataaatccct gcagtttggt tggattggca
     1021 gctttgaacc ggcattcaaa ccagattgtt tttccggccg ccggcttgac catcaatccc
     1081 tttttctgga cattgatgcc ctgatgttgg gtagaagcac cagcgtttag aacggccgtg
     1141 ccatattctt cgtcgccgag cgtaaatgtt ccgctggtcg cctgcgttgc agtatatcca
     1201 gccagagtgg cagcgtgata attattaaag tcttcaaaca gatatgtccc gatagacggg
     1261 tcattatacc acgcaagaag tgggcagtcc ttccagaggc tggacaagat agaaggatac
     1321 cggtcgtcgt aaataattaa attctttgat ttccaaattg attttgttgc cataggattt
     1381 tcctttcaaa ttttggaaag cgggcagccg ttggagaccg cccgctcaat ccgatttttc
     1441 agtttagtct gttaatggtt caacggtatc gtaatcatcc ttgaatctcg gttcactcaa
     1501 tacataagtg gcgctgtaat aatcattatt agcgcctggc gatgcaaccg caagctggat
     1561 acagtcacaa tcacccagtg aagcggcgtc aatctcgata acatacattg aattggccgt
     1621 gtccagattg aatgtattgc tggttacagc cgtctttgtc agcagtgaac cagtcgctgc
     1681 tccgtcattc gtgtacatat acgaaaaaga agcagccgtt gcacctaatc cggatacttc
     1741 ggtagcctta ttgaccgtga cggcagccgt gccgccagcc caagctcccg tgttgattac
     1801 gatagcacat ttgcgataat tcttcaagga aatgtaattg cctgtaccgg ccgcacctga
     1861 ataattagcc ggcggtatgg cttctacgaa cttgattttg tcttgtacca tattcataac
     1921 tccttattaa attttagctt ctcgtcgcca aagcaacgaa ggcgcttaaa gtgttcgaac
     1981 cgtgtttcgg tgtaatcgcc gacttcatcc agggctggcc gtcgtatcgg agggtaaacc
     2041 ggaaagcgac ttcatcctca acgaatttta tatggatgct ggtagccgcc tgaatagccc
     2101 cgccggcctt ttcgccgaat agatactggg tcgggtcgat gcagatgata tcgccgaccg
     2161 taccaagcgt ctgggcatgc tcggtcagga aaagcggctt gtttatcaat gtctgctgcg
     2221 gcgtgccggt taagccgttt gttgacattt gaagcaagcc ggccggaata ccgcccgtgc
     2281 cgactggaag cgacagcgaa gccaattgct caaaagtatc gttattggca atccagatag
     2341 cattcttttg gcaggccgga agcatccttg accacatttt caaaatgttt tcggccacaa
     2401 ttgtcgccgc tgcctggccg gtttctttgg ccaccgatat taagctgggt gaattgagaa
     2461 tacccagcgg ctggccgaca ccagttccgt tgatgatatc ttcatcaatt tggaagccaa
     2521 tcgcctcgcc gaaggttgta gcaaacagcg gctcgattga tatcgggcta tcttccaata
     2581 gctcgctgga tacatacgcc atagcggcca atttgttaag attcagctgg acgctgccga
     2641 ttttcggctt gctaccggta atcgttccgc cttcggtggg cctgtaaact acgataccgc
     2701 cgtatacgct ggcggccctt gaagtttcgt caatcgtcgg gattttgacc gaattggttt
     2761 ccatcgggat acgggtaaca cggcccagca ggacggaatt ttctacagcg actcgcatca
     2821 attcagaccg gtaagccgtc ggaacaagat aaccgccgtc gctgccgact gactcctgca
     2881 ttccggacgc tttggtgttc agatagttag tccatttgcc caacgtttcc ggcatagacc
     2941 ggctgacgga tgcccgatag acttcgtggg ccagatgccc gatagactta aatccgccgg
     3001 tcttctcata atcgctgctt tccgatgcct gatgaaaaga agaatttttt cgtgcataat
     3061 tttgtatctg ctctttaatc ttttcattga taagccgaag caggtcttcg gtcagcgaat
     3121 attgggcttc gttctttacc gtgctttcgg caacgacatc gctttgctgt ggttcataga
     3181 tttcagcaac ggcattttct atcaatgatt ttgctttttc ggcgggaatt tccaatagtt
     3241 gaccaacggg ataattcttt gccccgctgt tccattcttt gataagtctt actaacattt
     3301 tggtttgtcc ttaaaaaaat agttgacgat ttattcacgc ttcatatctc cagcgcttat
     3361 tcgtctaaca tctccggaca aacctgatgt ctctgatgca agcggcctga tatgtctcta
     3421 ctttataatt tcgatttctt ttatctcggg ataaaattcc cgtacatcca aaataatttc
     3481 ttcctctttt gtctcatcgc tcttcaattt gaattcatta tacagcttct cggacaatgt
     3541 caagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcacat cggcaatttg
     3661 gatgcagcgg cggatgtgca atattttcat aatccagctc gatatccgta tctttgacgg
     3721 ttaatgtatc acctttttgg ataaaatcgc tgtccaaact taccttcttg ccatgcatcg
     3781 acctgcagaa tggacagctt ctgtcatcgc cggagctgtc ccagacttta taagcgacaa
     3841 cgccggactg cttccacgcc tgttcgatgc cttcgttatg tgcccagatg gtttccgacc
     3901 gtgcgactcg ctcggcatcg gccaatgtgt tgaaattgtc ctttatcaag tcggcaattt
     3961 ttgccgctcc cataccctca ttgatgccct gctcgataag cttgcggatt ttcctctcgc
     4021 tgttggatac aagagtagca atttttcctt ttcgcttttc aagggctcgc cgcacttccg
     4081 gactgctggc atttatcacg ccgtctggct tgattttgga catctcttca atcattcgat
     4141 ttatcataat cccgcgcaaa aacggcgata aggtttcttc aagccgtttg gtccagtgtt
     4201 tgttgtcaaa gaccgtgctg ataacatcgt tgacaacggc cttttcttcc agctttttta
     4261 actttcggcc tatctcggaa atcatctctt tgtagaacaa taccagatgc gttattagga
     4321 tggcattttc cttgtactgc ggcggctcgg caggccgata cgatttttcc gatgtttctt
     4381 cagccgtcgg tgtttcagcc tgtatcgtct gagacagcgg attgaccggc gtttcacccc
     4441 attcgaccgg aggaaatccg tcgattgccc tttcctcgtt aatactggta taattgaccc
     4501 gcagacgagt ttcaatctgc cgtaacctga attcttcatc cggcggtctg ggattgtcga
     4561 acatcagcag cagactatcg tcaaataacg ggacaagccg ttcgttcaat ttgttttcca
     4621 gcacaaccaa tttaggccgg atgacctgct gtgcccaaag gtcaaggctt gcccaagcat
     4681 tcgcccgact gatttcctga attttcacaa atgacagcgg cacgccgaat acgccgcata
     4741 tctcttcgac cgccgatttg cggccctgca gataattcat atctttaggg gcaaatccga
     4801 ccggcttgat ttccgctccg ccggttaata tagacagttt gccccgtctg tccgggcctt
     4861 gaaactgctt gtatttggtc aatatccgcc gctcctcttc ctcgctgata aacgcatccg
     4921 gcggaaattg gatgacggcg tctgggatac cgccgttttt catcaacgag atttcggaac
     4981 ggttcataaa atcgtataaa tcggctgcct gctcgcaggc ttgaagcggg cttgtgccga
     5041 gataggggtc ggtcaatgat ggatacttaa agtgtattat ctgctcggcg gtaaattcca
     5101 tttgtttccc gccatgctga tatttgtaat aaatttcatt gtctattttt cgaatagaaa
     5161 caaattgcga taacagcggc cagatattca aaacttgatt gttttctgtt tccaaatacc
     5221 aaaaagcgtt gccgatggct tccaaatagc tgactgttat gtttaataat tcgtacagat
     5281 tgttgaccgg attgggccgc ttcaggatgt ccaacagcgg atgttcgaca agttcttcaa
     5341 tatccgaaac ggcatccaga tatacctttg atactatccg ctggacagca tttcggtctg
     5401 caggccgaga tttgtatttg ttctgctttt ttgtctgata cagccgcagc ggtgtttgtg
     5461 cacacgccgc cgcattgcgg tcgatgcaga catagaccca attttttact ttctggatta
     5521 aatcttccgg acgtccgctt gaccgccaat agaagggctc gtttcctatc gtatagaccg
     5581 ccggccgtgc gtccgccggc ttgaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnccgt ttttgtcctg attggcatcc aatatcgacc ggattaagtc ggcaagctct
     5761 ttgtttgtca gattcggctg ataaagatgc tctttgacat atagtgtttt tccgattatc
     5821 cggcactcga caaaagctgc ggggtcgatt gaataaccaa aatccagccc gtaataacga
     5881 atatcatatt tatccggtac agtttcagcg gtgctgtaat tttcaaatat acgcccactt
     5941 aaaacgcccc acttgccgag cgtataaatg ttgtaataat tttggtcttc ctctattaag
     6001 tgttcgagac gctcgatata agcatggtca ataaaacggt tatctttata agtcgagtgt
     6061 acaatcgtga tatcactatt ggctgtatac ttcctggaaa caatttgttt tccggattca
     6121 atttcctttt gtatatcatc atcaaagaat tgttttttta tccaatgctg ttcattgata
     6181 ggattaaaag tcattaatat ttgtttatac tgattcgatt tgccacgcag ccgcaggtca
     6241 atttgccgga aatcgtctaa tgtgaattca gttgcttcct ccatccagac tgatgttatc
     6301 ccgtgaatcg actttaattt ttcggggtcg tccagcccca taaataaaat ttcggatgaa
     6361 tccggaaaaa ccagttgcaa tcgagtttta ttttccgtta ctatttggga taatgaccat
     6421 ttatataata acgattttat ttcttcatat gcacttttcc taactgctgg ggttgtttta
     6481 cgcatacata agaggcggac aggcttttta gtataaaatg cctggataat gcggataatt
     6541 tgcttttgtg ctgcagctac agacttacca gagcccgcac cgccataata aattaaatac
     6601 cgactcctat tatttataag tttaaaatat aattcattta taaatttaca caaaccgctt
     6661 gtttgtatta aaatcatttt ttatattttt ttacttcatc aggcaatata tatggaattg
     6721 taaatttcca atttatctga tggtgtatcc tggaaaatgt atcttgaatt ataccgattt
     6781 ttacactcga tggcattgct aataatgtat aaaacgtctt tatataggtt ccaagttcca
     6841 gataactact tgttaatcca ccttcattat gctgtgttat taattgtaca atacttattt
     6901 ccggaatagt taaaaataaa taaccgatat tacctaattt agtataaaca tttacatctt
     6961 cattaagccg tccaaaaaat tcaaaccgcc tatcagtagc acaaaaaaat acattcatta
     7021 ttttacgtag ccgtcggtaa ctaaatgcgc gagtcatatt accgcctata aaatcacccg
     7081 cctgcgacat tgccaaactg tggatattag gtaatgcttt aagagtttcc ttatataaat
     7141 ataatatttt atctaaactt ttatgtaaaa tttttcctct taatttatta ttttgcatat
     7201 accgatattc aaaatttcca tagtcatcat ctaaaactaa aaaaaatgga taacctaact
     7261 ttttcgctat atcaaatact gcgtttctcg gaaaaaccgc cgatgataat ttatctatcc
     7321 ggttatccat ataatcgaat ttacctatat attctcgttt tgaataaatt acgaggttat
     7381 tttcgctaaa attcttttta tactggttta tttgtgtatc atcatcgcta acaacaataa
     7441 atataggata tttacaatta catttttgta aagttttata tgtttttaca ttatttgccc
     7501 gctgatatga gataataaaa atacaaaaat catttggatt atatatatta tctatcatag
     7561 acttattcat ctaaatcttc ggataagatt tcatcagcca ttatatcttt aattttttta
     7621 tgtatcacaa ccattccatt agctattgca ttctttatat caataattat taaaaccaat
     7681 ttttccataa tatcttggac ttctgctggc tggtgggcat aatattctgc tatattctgg
     7741 aaattaaatt ttataaaacg agttgctgct aattgcaaaa attctattaa ttttttatct
     7801 ttaatgccag tttcttctat ttgttttaat ttttgtttat agatagtaat atcataaagt
     7861 tcatttattg ctggcggatt ttcctgcatc ggtttatata caggtacttc aattttttgc
     7921 gaataaatag tttcttttgt aatatcttgt ttttcaatac tttcgctttg taataattta
     7981 tcaaaatcac aattctttaa taaaaaattt aattctttat tatctattaa cgcatccagt
     8041 tcagttttaa gtttttctat atcccattct gcgaaatctg ctgtccgatt gtcaataagc
     8101 cggtaagctc ttgtttgttc cggcgtcaaa tcggaggcga tatggaccgg cactttttta
     8161 agccccaatt tccgtgctgc cagccagcgg gtatgtcctg caataataac accgtctttg
     8221 tctatgacta tcggctgccg gaatccatac ttttttatgg actccgctac cacatcaaca
     8281 gcattttcgt tttttctggg attatctttg taaggcttta tgtcatcaat ttcgtaaagt
     8341 attactttca taatcaatca ggcttcaaat catccggtaa atcaacaaca attttcaaat
     8401 cgctcgacac ttgattatct atcttgtcct tccaccgtcc aggctgacga tttttcagat
     8461 aaaagattat agctgccgga ttgggcggaa gctgcttttt atactttttg acccgtacac
     8521 gtccggtctt ttcatccttt tcggcaacga tatcctcgta ttcatagccc aacgcaagtt
     8581 cgaccagctt gcgttcgacc ttttccgtgt cgaataaatc tcgacccgaa cagattgctt
     8641 ccttgaagtc cggctgctta tgcatccagt catagattgt gtcaacatgc accccgaaca
     8701 gattggccaa tgcggcgata tccgccccgt ttctggaaca ggcattatac gccagtttga
     8761 catagtccgg tttgtaattg gattttcgtc ccatctcttt ttatggtatc ggattatccc
     8821 gaacaccttt tatatatagc tcaaccttgc tttttagccg gctaccatca atacatcgat
     8881 tctcaattgg caattgtact aatcttaaaa attcatcaac ttctttggca tttttgaaga
     8941 caatttacgg caactataca ggcatccgaa tattcggccg cctttttaat caatgactgc
     9001 ggcctgctcc ggtcgccaat ctcttgcccg ctcgcttttg cggcttcgaa cgtgatttcc
     9061 tcgatgtttt ttttgccata ctctttattt ataagtttat tttggcctct gtcaatctat
     9121 tatttttgca aaaatccgaa tctcttcttt ttctgcatac tttttttcgg ccagcaattt
     9181 tacaatttgc ttatcatcct cccaaataat cccgtttaga gcgtccaaaa ccagtttggc
     9241 cagattatct atatctgcat atttcgggca atacccgctc gttttgcctt tgtaataaaa
     9301 ttctattttc acctctatcg gtccgcacaa taattgtgct ggtcgaggat aaatgaactt
     9361 ggcaatctca ttttttatct tttgtgatgg attcacggcg taaatccttc cgtcccgacg
     9421 atagactctt ggtctttgct gcggaatcgg cctaataggt atattgtaat gaagcatacg
     9481 atttcttttt gtcttagcat tttataaata gcagctcaca gacagcctta tgattaatct
     9541 gtaaaagatt gttttacatt ggcatctctc cgattatcaa gctgctgctt gattcgctca
     9601 actttttttt gagcggcaag gattttatat aggatttcca gacgccgcat ccgttcttcg
     9661 ataatttgca gttttgattt catactatcc aatatatgcc ggcccccaaa aaaatttccg
     9721 caagattaac gcaagattaa cgcaagatga atgtagggcc gcaaactata tcgtaagtcc
     9781 ttgtgtgaga tgattttatg aggatacatt ctgcgatgca gggctatttc ccatcatcgc
     9841 ccgttccccg tgcccgctta gtcaaccact gaactaaaac ttccaagtca aaaaggactt
     9901 tattgtcggc aataagagcc ggaattgccc cacttatcgc ctgctcttta agccatttgg
     9961 gaggcaggcc aaaccgccga gcagcggctc ggagatgaat taagttgtcg attttccgtt
    10021 cattttccat cctgtacact ttcaaatttt ctttttcttc ttggcttatc aatttattac
    10081 tccttaatcg gcaataattg ctcttctgct tctatgtatt tgtcaatgta gcgactgcta
    10141 taatctacaa ataccttttc ggtactcttc tttgggtcta tatttgggaa tcccaaatct
    10201 acaaatttga ccttacttcc ggctggaaac aaatcttttg ctgcctgctt ctgtttgtcc
    10261 gtccatccgg caatcggatg gttggagata ttaacaaatt ttgtcataat tttccccttt
    10321 catcagaatg ttctcgtcat tacagtac
//
LOCUS       DAOTBO010000094        10316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5507_length_10316_cov_5.751584, whole genome shotgun sequence.
ACCESSION   DAOTBO010000094 DAOTBO010000000
VERSION     DAOTBO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_10316_cov_5.751584"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="PLE88_10110"
     CDS             complement(<1..1463)
                     /locus_tag="PLE88_10110"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM76858.1"
                     /translation="MRSKSKWLWVAVWATVMATALQASAASISMNFVENATNQILTGG
                     TPIGPISVDGKYWNDNSKVGTPWATNSMSNLIDDTGAATGVNVSWRSSNTWYTGEGTG
                     SDEAKLSVGYLDDGTTTSPYGVQVTFTNIPYIRYRVYGMYASDTNQNTSPYIVTMRNF
                     EVNGVWVLGGTDATTAQAYGRTDRNYTANGQYWTKIEPGVKVGNYWTIETSGSTLTIK
                     GLPRNGSQRGSLTGVIIEEIDKFLAYNPTPAVGTEVPLNQVLSWQQVPEVSGLGVTYK
                     VYFGTDPNVLSPNYYGNQLVKTTTSDPADFFYAPALNNSTKYYWRVDAIEPNLPNPIV
                     HTGQDWWFTTQPPSPRIETNPVSQTVPAGTDVQLSVSGINITSYQWYKDGAALPPAPA
                     LYAGEQTSTLTIFDVQVADEGFYYCVGDNSLNQPSTSAAAQVLTERLVGWWRLDGDLT
                     DSVDEVIAGAAAHDGVSTDPNFVLGKDGSALQFFGGA"
     gene            complement(1794..3488)
                     /locus_tag="PLE88_10115"
     CDS             complement(1794..3488)
                     /locus_tag="PLE88_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM76859.1"
                     /translation="MPLDELGNLDELILKEIDGIITEQEFAELTDCLCRNPEALNYYL
                     DFITICAGIFKPGEVTFCQTLCSAAEDDISQSALWMALAENERTAEAVPLEKSDPLPL
                     LSIQPQASCRRQNVSRAALYTAIVSTAALLLMIAFVVTHPRQIRDAVASLSGSSQAVW
                     SEHFCPPKDSSRLYTHSTLSLLEGFAEITFDNQAKIILQSPVWIDIEDYNQLFLHSGK
                     LSAVIPTSAAGFVVRTAAATVVDYGTEFGVTADADGHTEAHVFEGKVELRSGSDPIRF
                     AEKLNLTKGWAGCVSRTGQLSSEPVPAQPLSYIRRLPAKPTFGQPGKRLDLADIIGGG
                     NGFGTGKPCVGIDPASGKIVSDLVLVKRKPEQLHYFSVPEIPFIDGVFVPSGGNQPQI
                     VTSQGHTFNQCPETDGSYWIEITNNPIASVLGNAGGQAGDIHLVRLNGIQFGSALHPG
                     IMLHSNAGITFDLDAIRSSIPGARISRFTSICGMSDTIIQQNVQAAIWVLVDGQKRQE
                     AHFIYNRTSHSAYIDVDIHDQDRFLTLITTDGGDSNGSDWTFFGDPALELELSGVR"
     gene            complement(3488..4048)
                     /locus_tag="PLE88_10120"
     CDS             complement(3488..4048)
                     /locus_tag="PLE88_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76860.1"
                     /translation="MNQMDPQSEGKRVEKSIKDDFISLFLSNQRRIFTFILMYVPNRS
                     DAEDLMQETASWMWNNFDTYKPGTNFGAWGVQVARYKILKLHSRTQHSRLKFDSQLVE
                     LIDARAEKVLDDVPQKIHALRKCLSEINEQSQRLLYLRYEKGLTPQKLAELLHRPVQG
                     LYKTMARIHKMLLRCVRQKLLAEGEL"
     gene            complement(4591..4664)
                     /locus_tag="PLE88_10125"
     tRNA            complement(4591..4664)
                     /locus_tag="PLE88_10125"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4628..4630),aa:His,seq:gtg)
     gene            5201..8518
                     /locus_tag="PLE88_10130"
     CDS             5201..8518
                     /locus_tag="PLE88_10130"
                     /inference="COORDINATES: protein motif:HMM:NF020768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HOM76861.1"
                     /translation="MKRRIAFAMMLALAFMLPLAASMMAAQDARTDKAVPARPEGPCD
                     IYAAAGCPCAAAHSTTRALYASYNGPLYQVMRQSDGKTLDIGVVQPSEGDAGGYADAA
                     AQDAFCADTYCWITTIYDQSGNGNHLVQAPRGGFSGPAMGGFNNIPIADMAPVTLMGH
                     KVYGVFIEPGMGLRWNDAKGTAVDDQAQGQYWVVNGHHYNSGCCFDYGNAETDSRDDG
                     DGTMETTYYGNAGAWYRGQAPGPWIMTDQENNLVGCVNPSPNDKFCKDLPTITWRFVT
                     ATADGQPHHWRTMGGDAQRGQLQVLFDGPRIINDRSSYDPMRKQGAILLGNGGDNSNG
                     SQGTFYEGAMTAPNTFPSQETQQKVQANIVAARYDVQRLSIAPAAAAAAPPGLQTFRP
                     GSSQDTTVTFTNTVGVHVADLRLAIKAPQGWTVVAKTPARIAGAIAPGQTVSATFTVT
                     SGPKPFNGDLVGTASWTNTSNGRRESESAVEKVRNVSPVKINEFAVGSGSPANPTNSF
                     IELYNAGDSTVDISGWTLTQHPTQQPIFSSVKIPAGTTLAPKGFYLLALSDSGLAVPA
                     KTGDTVIYVRSIEGMSVGDTIEIDTGSSVEVRKIAGIQSPSADEPAAGRGGFGGRRGG
                     IRGLTTVWQPLPDGPVITIPAGSTNIPVTSVDGFRVGQKMAIGYGATYPAVAQAVEKY
                     EVVTITSVGKPGTQAWLSMDAKAGDTNIKVSSVENISVGDKIRLDIDSVGHGIETVTV
                     TKVGTQSVRSTFNGPLREGEDPGTGLDLAEPLKFNHSSNMPFSVRGTGISFEPPTAFN
                     HSSNEPVLALNTSITLDKPLDKDHDINAPVRDEKVAAAGYQGAKKPNQWFGGPALAGA
                     GCMVLRDADGLVVDSLNYGGLVDPWAAEGWQAASGAGESGCYAPAPIAGFGGRGGFGG
                     RFPNRSAGRFPDGADTDTNCYDFLLQTATTLAAASAEGAANIKVISVADFSAGQTIII
                     DTGANRETAVIAAVGTAGGTVLSAAVEAGATVIPVANAAGFAVGQTITIDSPSNQETA
                     VIASINRGRGGFGGAPGAASITVASPLAKAHPAGAEVAGTGITLASGLTKSHESGAQI
                     AGSEPTPGEPNQYSRPL"
     gene            8648..9940
                     /locus_tag="PLE88_10135"
     CDS             8648..9940
                     /locus_tag="PLE88_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOM76862.1"
                     /translation="MVSNEQTDHCCRYWPWWRWILTGMSALGLALSLYLSWHDIADAP
                     MIGCSGGSSCDRVLSSRWSSIAGLVPVSGLAAGAYGALLTAVFFIGTAAEKPIRHLAW
                     AVLLVIAGAAAGCAVWFTILQKWVIGAFCPYCMTIHLLGLLLAVLVVWRAGKECSGAL
                     TDPETMPPASPAGRDGADSVPSQKGSSAPSGQNRLHPFGLFLTGLLTAGILAVCQTVF
                     APPAEWLDGQSEQYQPAVDPHAVPLIGSPEAPYVVVLLFDYACSHCQQMHFMLEQAVS
                     RCGGRLAFALCPTPLNSRCNPYIAQDADEFRDSCQLAKTALAVWLADRRKFAEFENWM
                     FSFESGDRWKPRRIEAAEAKAAELVGQAAFDAARADSRIEEYLQTFLDMYGRTSQNAS
                     GAVPKLIFGSRWVIPQPHNADELVAILQNSLAIAGLDS"
BASE COUNT         2378 a         2795 c         2732 g         2411 t
ORIGIN      
        1 tcagcaccgc caaagaactg cagtgcactg ccgtctttgc caaggacaaa gtttggatct
       61 gtgctgacac catcgtgagc cgctgcgcca gcaatcacct catccaccga gtctgtcaga
      121 tccccgtcca gcctccacca gcctaccaga cgctcggtta acacctgagc cgcagcagat
      181 gtagagggct gattcagact gttatcgccc acacaatagt agaagccttc atcggccact
      241 tgcacatcaa agattgtcag cgttgaggtc tgttcacctg cataaagagc aggagcaggc
      301 ggcagggcag caccatcttt gtaccactga tacgaggtga tattgatgcc ggataccgac
      361 agctgcacat ctgtgccggc aggaacggtt tggctgactg gattagtttc aatgcgcggt
      421 gaaggcggct gcgttgtaaa ccaccaatcc tgtcctgtat gcacaatagg attgggcaga
      481 ttcggctcaa tggcatccac tctccagtag tacttggtgc tgttgttcag cgcgggagca
      541 tagaagaaat ctgccggatc actggttgtg gttttgacca gttgattccc gtaataattc
      601 ggactgagta cattcggatc ggttccgaaa tagaccttat aggttacgcc cagaccgctg
      661 acttcgggca cctgctgcca gctgagcacc tgattcagcg gtacttctgt cccaaccgca
      721 ggcgtcggat tatatgccag aaacttgtca atttcctcga taatcacacc ggtaagcgag
      781 ccgcgctgag agccgtttcg tggcagtccc ttgatagtca gagttgagcc cgacgtttca
      841 atagtccagt aattgccgac tttgacgccc ggttcaatct ttgtccaata ttgtccgttg
      901 gcagtatagt tgcggtccgt tctgccgtaa gcctgagccg ttgtggcatc tgtccctccc
      961 agcacccata cgccgttgac ctcaaaattc cgcatggtca cgatataggg agaagtgttc
     1021 tgattcgtgt ccgatgcata catcccgtac acgcgataac ggatatacgg aatattggta
     1081 aaggtaactt gtacaccata aggacttgtt gtcgttccgt catcgagata gccgacggac
     1141 agcttggctt catcactgcc ggtgccttcg ccggtgtacc aggtgttgga gcttcgccaa
     1201 gacacattga cgcctgttgc ggcaccggta tcatcaatca ggttggacat cgaattggtc
     1261 gcccacggtg tgcccacttt ggaattatca ttccagtact tgccgtctac gctgatcggg
     1321 ccgatcgggg taccgccggt cagtatctgg tttgttgcgt tctcgacaaa attcatcgag
     1381 atagaagcgg ccgatgcctg caaggccgtc gccatcacgg tcgcccacac agcgacccaa
     1441 agccatttgc ttttacttct cataatcatc actcctaaaa cataaacttt ttacactctt
     1501 gtttacttgt gtgccccttt tcattactgc ttttatcaca tgaatccgcc aaaacgatat
     1561 ttaaaaccac acgaacatct atttctgtac atgcagcaca gcgaatgcag ccctcctttc
     1621 ctgaaaaata gaataatcat ttgctatcta aatttctgta tcaatgcagc gtttatcgta
     1681 aaacccatat ggcattgcct ctgggaaaaa caccgccgca gcaaattctt ccccgcatcg
     1741 gggcgaataa tcccattctg ctattttatt atcaaagaaa gcatttcctg cggttaccga
     1801 acccccgaca attccagctc gagggcagga tcgccaaaaa acgtccagtc gctgccgttg
     1861 ctgtctcctc cgtcggttgt tataagcgtt aaaaatcgat cctgatcatg aatgtccaca
     1921 tcgatatagg cagaatggct ggtccggtta tagataaaat gtgcctcctg ccgtttctgg
     1981 ccgtctacca gaacccatat agccgcttgt acattctgct gaataatagt gtccgacata
     2041 ccgcatattg aggtgaatcg gctgatccgc gctccgggaa tcgaggaccg aatagcatcc
     2101 agatcaaagg taattcctgc attcgaatgc agcataattc ccggatgcag agcggatcca
     2161 aactgaatgc cgttcaaacg gaccagatgg atatccccag cttggccgcc ggcattccct
     2221 aggacactgg caatcggatt attggttatc tcgatccaat aggaaccatc cgtttcgggg
     2281 cactgattga acgtgtgccc ctgcgaagtg acaatctggg gctgatttcc gcccgaaggt
     2341 acaaagacgc cgtcgatgaa gggtatttca ggaaccgaaa aatagtgcag ctgttccggt
     2401 ttccgcttga ccagcaccaa atcgctgact attttgcctg acgccggatc aatccctaca
     2461 cagggcttgc ctgttccgaa tccgtttccg ccgccgataa tatccgctaa atcaagacgc
     2521 ttgcccggct gaccgaatgt cggctttgcc ggaagacggc gaatataaga cagcggctga
     2581 gccggcaccg gttcggatga cagctgcccg gttcggctga cacatccggc ccagcctttg
     2641 gtcagattca gcttttcggc aaatcgaatc ggatccgatc cgcttcgcag ctcaaccttt
     2701 ccctcaaaga catgggcttc cgtatggccg tcagcatctg cggtaactcc aaattccgtc
     2761 ccgtaatcca ccaccgttgc cgcagccgtg cgcaccacaa atcccgcggc ggatgtggga
     2821 atcacagcgg ataatttccc gctgtgcagg aatagttggt tatagtcctc aatatcaatc
     2881 cagaccggag attggaggat aattttggcc tgattatcaa acgttatttc cgcaaatccc
     2941 tccagcagag acagggtcga atgcgtatat aagcggctgc tgtctttggg ggggcagaaa
     3001 tgttcggacc agactgcctg actgctgccg gacaggctgg caaccgcatc ccggatttgg
     3061 cggggatgcg tgaccacaaa tgcaatcatc agcaataacg ccgccgttga cactatggct
     3121 gtataaagtg ctgcacggga aacattctgc ctgcggcacg atgcctgcgg ctgaatagac
     3181 agcagcggca gaggatccga tttttccaga gggacagcct ctgctgtccg ttcattttcc
     3241 gccaaagcca tccagagagc cgactggctg atatcatcct ctgctgcaga acagagagtc
     3301 tggcagaaag tcacttcgcc tggcttgaaa ataccggcac aaattgttat gaagtcaaga
     3361 tagtaattca atgcctccgg attccggcac agacagtctg tcagttcggc aaattcctgt
     3421 tcggtaataa tgccgtctat ttctttgagg atcagctcgt ccagattccc gagttcgtcc
     3481 agaggcatta cagctctcct tctgcaagta atttctgacg aacgcaccga agcagcattt
     3541 tatgaatgcg ggccatggtc ttgtacagcc cctgtacagg acggtgcagc aattccgcca
     3601 gcttttgcgg agtcaatccc ttttcgtatc gcagatacaa aagccgttgg ctctgttcat
     3661 ttatttcgga aagacatttt cgcaaggcat gaatcttctg cggcacatcg tccagaacct
     3721 tttccgctct ggcatcaatt aactctacca gttggctgtc aaacttcaat cggctgtgct
     3781 gggttcgact gtgcagtttg agtatcttgt accgtgcgac ttgaactccc cacgccccga
     3841 aattagtacc gggtttatag gtatcaaaat tgttccacat ccacgaggcc gtttcctgca
     3901 ttagatcctc ggcatctgaa cgattcggaa catacatcag gataaaggtg aaaatccgtc
     3961 tttgattcga caaaaaaaga cttataaagt catctttgat ggacttttct actctttttc
     4021 cctctgactg ggggtccatt tgattcatgc ttcctaaatc ctgcttcaag taaatttggg
     4081 caaaggactt gcccttgttt tctatcgctt aatacctttc gatgcctgaa ttatccccca
     4141 atttttttaa tttttttatt ttaataaaaa aatactattc ctatctatat ttaggtaata
     4201 ttaataaagt attataaaaa tcgcaaaaac tgataatcct aactcgaggt ttcggctttt
     4261 tctattttgt cacaatgcag caaaagaatc aagaaaatag gcatgatatt tacataacca
     4321 tttaatatga aatgatttat aaatattgcc aagcagccgg taaatccgtg taggcatcta
     4381 tactattgtt cgctaagaag tcacatttaa aatggataca aacgataaac aatcaataag
     4441 aagtgtttgc caaatatact gtaaggattg tttaacaagc cggcagaaaa aaaggcatgc
     4501 gaatgtaaaa taaaattaca ccgcaattta tacaccaagg aaaagccaga aaagaccgat
     4561 aatctgcctt ctattgaggc taaaaatcag ggtggaagac gagactcgaa ctcgcgaccc
     4621 tcagaaccac aatctgatgc tctaaccaac tgagctactt ccaccgcttg gccggcagat
     4681 gataaaaaac ctcccaaccg gcaaaacaca aatagtacct gatacatgca aatctgtcaa
     4741 tacagacatt tggtttttac tgtaaatacc tatgtctttc gatatttagt aaatccaatc
     4801 gcacagtcag gatactggcc cagcaggcac tgttccggcg gatgataagc agatgtcagg
     4861 agggcaaaag gaatactcgt gcagggcaag gcgctgcaag tttttcctga aaagaagttc
     4921 tgtaaggcag ccatttgctt caagccggcc gggtccattg cagccgtcat acccgggatg
     4981 ggggatattg tgaaagggtg atttaaaatc gctttgaaga ggattattgt tgatagaaag
     5041 acggcggcgg tttaaactac gaaactgttt tgaatcatat ggggatttca agggacccgg
     5101 acgagcttaa attggtcagc ttgagggaat gtcttttttt ctgccgcagg tgttttgcct
     5161 gtgtgcggac aagcgaaaca ttttttgaaa gggaaaggaa atgaaaagga gaattgcgtt
     5221 tgcgatgatg ctggcccttg cttttatgct gcccttagcc gcatcgatga tggctgctca
     5281 ggatgcacga acagacaagg cagttccggc cagacccgaa ggtccatgtg atatttatgc
     5341 tgcagccggc tgtccttgtg cagccgcgca cagcaccacc cgcgccctgt atgcctctta
     5401 taacggcccc ctttatcagg tgatgcgcca gtcggatgga aaaaccctgg acatcggtgt
     5461 ggtccagccc agcgaggggg atgccggcgg ctatgccgat gcggcggccc aagatgcgtt
     5521 ttgtgccgat acttattgct ggattacaac catctatgac cagtcgggca acggcaacca
     5581 tcttgtccaa gcgccgcgcg gcggtttcag cggtccggct atggggggct ttaacaacat
     5641 cccgatagcg gatatggcgc cggttacgct gatgggacac aaggtctatg gcgtctttat
     5701 agaaccggga atggggcttc gctggaatga tgccaaaggc actgcggttg acgatcaggc
     5761 acaggggcag tactgggttg tcaacggcca tcattacaac tcgggctgct gctttgacta
     5821 cggcaatgcc gaaaccgaca gccgcgacga tggcgatggg acgatggaaa ccacctacta
     5881 cggcaacgcc ggcgcttggt accgcgggca agcccccggc ccttggatta tgaccgatca
     5941 ggaaaataac cttgttggtt gcgtcaatcc ctctccgaat gataagttct gcaaagacct
     6001 gccgactatc acgtggcggt ttgtaaccgc aacagcagac ggccagccgc accactggag
     6061 aacgatgggc ggcgatgcgc agcgcgggca gctgcaggtt ctgtttgacg gcccgcgcat
     6121 catcaatgac cgaagcagct atgacccgat gcgcaagcag ggggcaatcc tgctgggcaa
     6181 cggcggcgac aacagcaacg gttcacaggg caccttttat gaaggggcta tgacggctcc
     6241 caacaccttc ccgtcacagg aaacccagca gaaagtgcag gccaatatcg tagcagcccg
     6301 gtacgacgtc cagcggctca gcattgctcc ggcagcggcc gcggctgcgc cccccggact
     6361 tcagactttc cgccccggtt cttcgcagga taccactgta acattcacca ataccgtagg
     6421 cgtgcatgtt gccgacctcc ggttggccat caaagccccg caggggtgga ctgttgtggc
     6481 caagaccccg gccagaattg ccggcgcgat tgctccgggc caaaccgtca gcgcgacatt
     6541 taccgtaacc tccggcccca agcccttcaa tggcgacctt gtcggcacgg cctcgtggac
     6601 aaatacctcg aacggccgca gggaatcgga gagtgctgtc gaaaaagtcc gcaatgtcag
     6661 tccggtcaaa atcaacgagt ttgccgtcgg ttcaggcagt ccggccaatc caacaaattc
     6721 gtttatcgag ctgtacaacg ccggcgacag cactgtcgat atctccggct ggacgttaac
     6781 ccagcatccg acacagcagc ccattttttc ctctgtgaag attcctgccg gcacgacact
     6841 ggcccccaag ggcttttacc tgctggcgct gtctgattcc ggactggcgg ttcctgccaa
     6901 aacgggcgac acagtcatct atgtcagaag catcgagggc atgtcggtcg gcgatacaat
     6961 tgagattgat accggctcct cggttgaagt ccgcaagatt gcaggcatcc agtcgccttc
     7021 ggctgatgag cctgctgccg gacgcggcgg cttcggcggc cgtcgaggtg gcatccgcgg
     7081 tttgacgacg gtgtggcagc ccctgcccga tgggccggtg ataacaatcc cggccggttc
     7141 aaccaatatc cccgtaacaa gcgtggatgg tttcagggtc ggccagaaga tggctatcgg
     7201 ctacggtgcg acctatcctg ccgttgcaca agctgtggag aagtacgagg tggttaccat
     7261 caccagcgtc ggcaaacccg gcacccaagc ttggctgtcg atggatgcca aagccggcga
     7321 taccaacatc aaggtgtcat cggttgagaa tatctcagtc ggcgataaaa tccgattgga
     7381 tatcgacagc gtcggccatg gaattgagac cgttaccgta acaaaagtcg gcacacagtc
     7441 ggttcgcagc accttcaatg gtccgttgcg ggaaggggaa gacccgggca caggactcga
     7501 ccttgccgag ccgctgaagt tcaaccattc ttccaatatg cccttcagcg tcaggggaac
     7561 aggaatcagc ttcgagccgc cgacggcttt taatcactcc agcaacgaac cggttttggc
     7621 gctgaacacc agcattacgc tggataagcc ccttgacaaa gaccacgaca tcaacgcccc
     7681 cgtgcgggat gaaaaggttg cagccgccgg ctatcagggc gcaaagaaac ccaatcagtg
     7741 gttcggcggt cctgctcttg ccggcgccgg ctgcatggtg ctgcgcgatg cagacggttt
     7801 ggttgttgac agtctcaact acggcggctt ggttgatccg tgggcagccg agggctggca
     7861 ggccgcctct ggggcaggcg agagcggctg ctatgcacct gcgccaatcg caggttttgg
     7921 cggccgcggc ggttttggcg gccggtttcc caatagaagt gccggccgct tcccggatgg
     7981 tgccgatacg gataccaact gctatgattt cctgctgcag accgcaacaa ctctggcagc
     8041 cgcttcagca gagggtgcag cgaacatcaa agtcatcagt gtggccgatt tcagtgccgg
     8101 ccagacgatt atcattgata ccggcgcaaa ccgtgagacg gctgtcatcg cggcggtcgg
     8161 cactgcaggg ggaacggtcc tcagtgctgc cgtcgaggcg ggcgcaacgg ttatccctgt
     8221 cgccaatgcg gcgggctttg cggttggcca gaccatcacc atcgacagcc cgtccaatca
     8281 ggagacggct gtgattgcct ctatcaatcg cggccgcggc ggttttggcg gagcgcccgg
     8341 tgctgcatcc attaccgtgg cttcaccgct ggccaaagct caccccgctg gcgctgaagt
     8401 tgccggtacg ggcatcacac ttgcttccgg cctgaccaaa tctcacgaaa gtggcgcaca
     8461 gattgccggc agcgaaccca caccgggtga acccaaccag tacagcaggc cgctgtagaa
     8521 atcagtactt cagcggcaga taaaactgcc gttcgaactg tctataacag tacggctccc
     8581 ggccctgcgg cttccaagcc gcagggccat ttgtaatgcg tgcagaaaat ccccaaagga
     8641 gcacattgtg gtttcaaatg agcaaacaga ccattgttgc cggtactggc cttggtggcg
     8701 ctggattttg accggtatgt ccgcgctggg attggccttg agtctgtatt taagctggca
     8761 tgacatcgcg gatgctccga tgattggctg cagcggcgga agttcctgcg acagggtgct
     8821 cagcagccga tggtcttcca tagcagggct ggtgcccgtc agcggtttgg cggcgggggc
     8881 atacggggcg ctgctgacag ccgttttttt tattggcacg gccgcagaga agccgattcg
     8941 gcatctggct tgggcggttc tgctggttat tgccggagct gctgcgggat gtgctgtgtg
     9001 gtttaccatc ctgcaaaagt gggttattgg ggctttttgt ccctattgta tgacgattca
     9061 tctgcttggt ctgctgctag cggttttggt ggtatggcgg gccggcaaag aatgcagcgg
     9121 cgcgttaact gaccctgaaa cgatgccgcc tgcatcccct gccggcaggg atggggcgga
     9181 ttccgtgccg tctcaaaagg gttcatctgc accttcgggg cagaaccgct tgcatccttt
     9241 cggactgttt ctgacgggcc tgttgacagc gggcattctg gcggtctgcc aaacggtttt
     9301 cgctccgccg gccgagtggc tggacggcca gtcggaacag taccagccgg ctgtcgatcc
     9361 gcatgccgtg ccgctgattg gctcccccga ggccccctat gtggttgtcc tgctttttga
     9421 ttacgcctgc tctcattgcc agcagatgca ttttatgctg gaacaagccg tcagccgctg
     9481 cggcggccgg ctggcctttg ccctttgtcc gacaccgctg aacagccggt gcaatcctta
     9541 tatcgctcag gatgccgacg aattcaggga ttcctgccag ctggctaaaa cggctctggc
     9601 tgtatggctg gcagatcgcc gcaagtttgc ggagtttgaa aactggatgt tttcctttga
     9661 atccggcgac cgctggaagc cccggcgcat cgaagccgcc gaagccaaag ccgcggagtt
     9721 ggtcggtcag gcagcgtttg atgcggcccg agcggattcc cggattgagg agtacttgca
     9781 gacttttctg gatatgtacg gccggacgag tcaaaacgca agcggcgctg ttccgaagct
     9841 tattttcggt tcgcgctggg tgatcccgca gccccataac gccgacgagc ttgttgcgat
     9901 tctgcaaaac agcttggcca tagccggcct cgattcataa cccatcggcg cgagagttat
     9961 ttcagaccgc ctttttcggg atttgctccc cgcattcaca gaaatcgctg cctgtgacgg
    10021 ctgagaataa atgcccaacc cgtgagattg ttgtgggcat ccgtaaacgg ctgtatattg
    10081 cccagcgatg gcaaatggat tataaccatc accggccgcg cagcagttag ggctgtcaga
    10141 cgcccgcggc gtacgcggcg tcttgtcgtg tttcggctcc ggcttgtccg gggccttcgc
    10201 ctttggcacg acggctgcat tgggcctgac tccgctgtgc tccgtcagct tcaatgcaga
    10261 gagaacggat cggctatgct ctcataagaa ctggtacaga aaaatggggc agatca
//
LOCUS       DAOTBO010000095        10251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5555_length_10251_cov_4.844547, whole genome shotgun sequence.
ACCESSION   DAOTBO010000095 DAOTBO010000000
VERSION     DAOTBO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_10251_cov_4.844547"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLE88_10140"
     CDS             complement(<1..247)
                     /locus_tag="PLE88_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM76863.1"
                     /translation="MAKQISGTEIVGKLRQADVQLAQGKTVPEVCRELGISQQTYYRW
                     RNKCGGMSVWIVNYSSGWKSCVTSVKNIGWIITTTGRI"
     gene            704..1387
                     /locus_tag="PLE88_10145"
     CDS             704..1387
                     /locus_tag="PLE88_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOM76864.1"
                     /translation="MARENILVVDDEEDVLELVRYNLHKEGYHVETVSSGEEVIKKAR
                     IKLPDLIILDLMLPGIDGLEVCKILKSDSKTRNIPIIMLTAKGEETDIVTGLELGADD
                     YITKPFSPKVLIARVRRVLQRMLAHELENTPLKIHDLVIDPSRREVLVKNKPVELTYT
                     EFNTLYTLAKRPGVVFTRYQIVDASRGVDYPVTDRAIDVQMVSLRKKLGSCGKYIETV
                     RGVGYRFKD"
     gene            1493..3295
                     /locus_tag="PLE88_10150"
     CDS             1493..3295
                     /locus_tag="PLE88_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM76865.1"
                     /translation="MIRRRLIWYLYPAFLGTGIVVLLAIACYAARSFHVFYYNQTVHE
                     LEVRARLIEHCIVSALQAGDYSQTDTLCKMLGRQSITRITVILPDGRVVGDSEENPSD
                     MEDHSRRPEFVAAMNAGWGKAIRFSRTVGKNTMYLAIPVKQNDATLAVIRTAIPVTQI
                     EQELTKLYARVFWAGVVIAVCAAAVSWYLSQKISRPIKDIKETAMRMASGDFSRRLSP
                     PSAAELAELSEAVNGMASQISSRIEIITEDKNCIQAILSSMIEGVIAFDSEGIIVWMN
                     KAAAALWEIDEVKSLGRNVEEVIRNPAFQEFAQKTLQEKEVPAQTVTLNATPGPKKLL
                     QLHGTALDAGKDGKKGAVIVMHDVTQLHKLEEIRRDFVANVSHELKTPITSIKGFVET
                     LQEGAIDDPQEARRFLEIIQRHADRLNAIVDDLLTLSRLEEDSENRRVFFETVYLRPI
                     LAAAIELSEYKANKKNIPIELRCEEGIAAKINPALIQQAVFNLIDNAVKYSPDHCPVT
                     ITVQQDTAEILISVSDKGCGIEEQHLTRIFERFYVVDKSRSRKLGGTGLGLSIVKHIA
                     QVHGGFVTVHSRINEGSTFTIHLPGGGSTFDKIN"
     gene            3482..4753
                     /locus_tag="PLE88_10155"
     CDS             3482..4753
                     /locus_tag="PLE88_10155"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOM76866.1"
                     /translation="MTRIMSVWVVGIVFLIGIAAADEVSELRKQMEQQYEQMQQMQNK
                     LNELEAAQKQQSMTVAKLEETGGTSLPETLKWLEKIKLYGDFRYRYEMVDEDGSDNRN
                     RNRIRARLGLTAKINDEIDTGFRLATSELWGDDEDSGDPVSTNQTLDDAFSKKSIWLD
                     LAYFNWHPAGIQGFNVIGGKMENPFYRVGGNQLIFDSDLTPEGIAFQYTHGWTEQTSW
                     FVNGGGFYVDEVKSGADTSLWGVQGGIKHTFQDKSYLVGGGSLYCFGNIQGHGAFVTV
                     NGSDKFFGNSKSGGLYINDYDLTELFGEYGFKLADTPTAVFANYVKNTVAAGSENAGW
                     LIGTKYGKCKDPGSWELSYDYRDLKADAVVGAFSDSDFIGGGTDGKGHRFSAVYQLAK
                     NTQAGLTYFLNEKGHDDHDYNRLQLDLMFKF"
     gene            4836..5648
                     /locus_tag="PLE88_10160"
     CDS             4836..5648
                     /locus_tag="PLE88_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOM76867.1"
                     /translation="MKTLIAQFLTIGCVLVGSVAAAEKLQIDGSTTVGPIGDAFAEVF
                     KDKAAVTVSKTGSGDGAAALMEGRCDIAMMSRFMKNAEFKAAVDKGIMPVAHAIAMDG
                     VCVVVNESNPVTGLTTEQIRSIYKGEITNWKQVGGPDKAIVVISRDTSSGTYETFEEL
                     VMHKEKMGPATEYVNSNPQAHARVKSTEGAIGYVGLGFVDRYVKAIKVDGVMPDRKTI
                     ARGTYPLSRPLFLFTNGYPKLGSIVYQFCTFHLTEEGQEIIEAKGFVPVTAY"
     gene            5648..6655
                     /gene="pstC"
                     /locus_tag="PLE88_10165"
     CDS             5648..6655
                     /gene="pstC"
                     /locus_tag="PLE88_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOM76868.1"
                     /translation="MKDSSTQSVQKQQAGVFEFARPLCLTATEDFRGFIAAHLGQGLL
                     FVTASISVLAVFLIFFFVIGQSLPFFLNRGLAESVPRIKEFLTSTQWYPQRQAGPEYG
                     ARAIIIGSLYVTVCSLMLAVPIGLLAAIFLSDIAPFGLRQFCKPVIEILAAIPSVAYG
                     VFAVMVLAPWMQNTLGFSSGTNALNASLILSVMALPTIISVAEDALSAAGRDIREASY
                     ALGATRAETMLLVVLPAAHSGIVAAIILGMMRAVGETMVVWMASGNASRIPSPWWDLS
                     QSVRTLTATIAGELGEVPKNTDHYHVLFAIGLLLLVFTFGLNMCSEYFMGQFRKITGR
                     R"
     gene            6656..8284
                     /gene="pstA"
                     /locus_tag="PLE88_10170"
     CDS             6656..8284
                     /gene="pstA"
                     /locus_tag="PLE88_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOM76869.1"
                     /translation="MRTGMRPVFDCVFTLLSAASVAFLIAALGIFLIPLCGKGMKAVV
                     FRGTVEFRKMQSDRFNRRNEATLQNEILQTQQVRDEIYALVDHFSRGLDTQTLADQAK
                     DLYRKYGDELRSRDLSRQQFADLRAAAKDIRDHLLAAFDSTDKAVVEEHLDAIEGLRH
                     QPGFEKTPVETMFRLADDFREMIQKVDLSRREEYAEELAAVNKELFALFGPRPGQQRV
                     LAMESYGATRWDVAQKHLRYLIWQEKWVPSPDGQTLQKVLIRRDDPEKGSFAKTELAP
                     LFGLIEKNLAAALNPRLTFYWQYFIDDSTPGHYFGGVGPELLGTLMLTLLAMCFAVPL
                     GIISAAYLVEYAGDNLAVRMIRICINTLAGVPSIVYGLFGLAFFVHYITKQPCILSAS
                     LTLALLVLPVIIRAAEEAIRAVPQSYREAAVGLGAGKFRTFATVTLPAAGPGILTGII
                     LSVSRAAGETAPILFAGAVALGPVPNFTGKWWFQPTRALSYGCYDITVGDKLAAMVPH
                     NQFGMVMTLIILVVCLNITAILIRMRLSKKLRGG"
     gene            8370..9188
                     /gene="pstB"
                     /locus_tag="PLE88_10175"
     CDS             8370..9188
                     /gene="pstB"
                     /locus_tag="PLE88_10175"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOM76870.1"
                     /translation="MSGINIADAAKNIPSDNHIIPEKAIIRSRDFTFYYGQKTGVEKI
                     TMDIEMGSVTAIIGPSGCGKSTFLRSINRINDLIPHTHTEGLLEVGGQNVYAKTTNLV
                     NLRQQVGMVFQRPNPFAKSIYDNVAYGARLLGVRKKADLDEIVEKCLCDAALWDEVKD
                     NLHQSALALSGGQQQRLCIARTLATRPKIILMDEPCSALDPLATARIENLIAKLKGTY
                     TIVIVTHNMQQAARVSDRTAFFCLGRLIEYDMTDKLFTNPQQKATEDYITGRFG"
     gene            9190..9864
                     /gene="phoU"
                     /locus_tag="PLE88_10180"
     CDS             9190..9864
                     /gene="phoU"
                     /locus_tag="PLE88_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOM76871.1"
                     /translation="MSQHLQHEIENLKKSILSLGARIETAVREATLAIEQRDGILAQK
                     IIDYDDVIDQQEVEIEEECLKTLALHQPVAIDLRFIITVLKINNDLERIGDLAVNIAE
                     RAVFLAGRPKPDISFDFVGMASAAQIMLKNSLDALVNLSVPLAQEVCAGDNTIDAMNR
                     KMYVKIQQAILAHPEQIESLIHMLSVSRHLERIADHATNIAEDVIYMIEGRIVRHRIE
                     KNILLS"
BASE COUNT         2625 a         2404 c         2703 g         2519 t
ORIGIN      
        1 tgatgcggcc ggtggtggtt ataatccagc cgatattttt cacagatgta acgcaactct
       61 tccatcccga cgaatagttc acgatccaga cactcatccc gccgcatttg ttccgccagc
      121 ggtaataggt ctgctgacta atccccagct cacggcagac ctccggaaca gtcttgccct
      181 gggccaactg aacatccgcc tgacgcaact tcccaacaat ctctgttccg cttatttgct
      241 ttgccattgt ccgataacct tttaaaatca gtcctatcca gactgcttct gtcggctatc
      301 ctctcatcca acttggatca atgttcgggc agcaggccgc ccgcgcgata aacgtcagat
      361 tataaatatt gggattggtg acttttaata gaaaggtact aaacccgggc cgcagggggg
      421 caacaagcta cttcggatgg attagggaga tccgaacagg ccggcagttt ccggccaatt
      481 ctaattatgg tgtcaaagtg ggtgtgattt cataatctgc acacaccttc cacaagtaga
      541 ctaccggttt catctatcaa aaacaggcat acaataaatg cgatgaggag caatcgggtg
      601 cagcaaatgc gataagttaa caagactcta atatttttct aatgaaatat aaacaaaaag
      661 aggtattatt aaattcaata aattgtccga tcaaggaacc gctatggcga gagagaacat
      721 tttagtagtg gatgacgaag aagatgtgtt ggaattggtt cgatataacc ttcacaagga
      781 gggctaccac gtcgaaacag tttcttcagg cgaagaggta ataaaaaagg caaggataaa
      841 gcttccggat ttaattattc tggatcttat gcttccgggt atagacggcc ttgaggtttg
      901 taaaatcctt aaaagcgact ccaaaactcg gaatataccc ataatcatgc tgaccgccaa
      961 aggtgaagaa acagatattg ttacgggtct ggaactgggg gcggatgatt acattaccaa
     1021 gccgtttagc cccaaagtgc tgattgcccg ggtgcggcgt gtgctgcaaa ggatgctagc
     1081 gcatgaactg gaaaatacac ccttaaagat tcacgatctg gtgattgacc catcccgccg
     1141 ggaggtgttg gttaaaaaca agcctgtgga actgacctat acagaattta acacccttta
     1201 cacccttgcc aagcggcccg gagttgtttt taccaggtat cagatagtcg atgccagtcg
     1261 cggagtggat tatcccgtaa cggacagagc gattgatgtg caaatggttt ctctgcggaa
     1321 aaaactgggt tcgtgcggga aatatattga aacagtgcgg ggtgtggggt atcgtttcaa
     1381 agattaggcc tttaatgggc attcctgcga gaaaacacat gactgtcgat tgcaaccgtg
     1441 tggtgtaata gctgtgcagt accgaggaat gtcaaggggc atcagcataa aaatgattcg
     1501 aagacggtta atctggtatc tttatcctgc ctttctgggt actggaattg tggtgctgtt
     1561 ggcgattgcc tgctatgctg cccgttcctt ccatgtcttt tattacaatc agaccgtcca
     1621 tgagcttgaa gtccgggccc gcctgattga gcattgtatt gtatcagcac tccaagccgg
     1681 tgattactcc cagacagaca ctctctgtaa aatgcttggc cggcaaagta taacccgcat
     1741 cactgttatt ttgccagatg gtcgggtggt cggagattcc gaggaaaatc cttccgatat
     1801 ggaagaccac agccgcagac ccgagtttgt ggctgcgatg aacgccggct ggggcaaagc
     1861 gatccgtttc agcaggaccg ttgggaaaaa tacgatgtat ctggcaattc cggtcaagca
     1921 aaacgatgca acccttgccg tcatccgaac agctattccg gtgacgcaga ttgagcagga
     1981 gctcacaaaa ctttatgccc gcgttttctg ggcaggtgtt gtcatcgccg tttgtgcagc
     2041 ggcagtcagt tggtatctgt cccaaaaaat cagccgtccc atcaaagata taaaagaaac
     2101 agccatgcga atggcttcgg gcgatttcag ccgcagatta tcccctccaa gtgcggcgga
     2161 actggccgaa ctttccgaag ctgtcaatgg aatggccagc cagattagca gccgaattga
     2221 gataataacc gaagacaaaa actgcatcca agcgattctt tccagcatga ttgaaggcgt
     2281 tatcgctttc gattcagagg ggattattgt ctggatgaac aaggctgccg ctgctttatg
     2341 ggaaattgat gaggtcaaat cgctgggccg caatgttgaa gaagtgatac gcaacccggc
     2401 atttcaggag tttgcgcaga aaacacttca ggagaaggaa gtccccgctc agactgttac
     2461 ccttaatgct acaccgggcc ccaaaaaact cctgcagctt cacgggacag ccctggatgc
     2521 cggcaaagac ggcaaaaaag gagcggtcat tgtcatgcat gacgtgacgc agcttcataa
     2581 actggaagaa atccgtcgtg actttgttgc caatgtatcg catgagttga aaactcccat
     2641 cacttctatc aaaggatttg tggaaaccct tcaggaaggg gcaatagacg atccgcagga
     2701 agccaggcgt ttcttggaga ttatccagcg gcatgccgat cggttaaatg ccatcgtcga
     2761 tgacttattg acgctttccc gattggagga agacagcgaa aaccgaaggg ttttttttga
     2821 gacggtgtat ttaaggccaa tccttgctgc ggccatcgaa ctttcagaat acaaagccaa
     2881 taagaaaaat attccgatag agttgcgctg tgaggaggga atcgctgcca aaataaatcc
     2941 tgccctgatt caacaggcgg tgttcaatct gattgataat gccgtaaagt acagcccgga
     3001 tcactgtcct gtaacaatca cggttcaaca agacaccgcg gaaattctta tttctgttag
     3061 cgacaagggc tgcggcattg aagaacagca cctgacacgc attttcgagc ggttttatgt
     3121 ggtagataaa agccgaagcc gaaagctcgg ggggacaggg ctggggctgt ccattgtcaa
     3181 gcacatcgcc caggttcacg ggggcttcgt aaccgtccat agccgaatta acgaaggcag
     3241 caccttcacg attcatctgc ccggcggcgg cagcactttc gataaaatta actgaaagcc
     3301 tgaaaagtct ccgacggcat tccactttca tccagcggat gctgtttgtg cagcattaat
     3361 acaaatctaa cagcccgctc attatttgct aatacatttc catgatactc tcctgcaact
     3421 ctatgttgac gctcaaatcg gtttgagcac attcatcaga aacttttatg gaggtcaaaa
     3481 aatgacgcga atcatgagtg tatgggtagt gggtatcgta tttctgatcg gcattgctgc
     3541 ggccgacgag gttagcgaac ttcgcaaaca gatggaacag cagtatgaac agatgcagca
     3601 gatgcaaaac aaactgaatg agctggaagc tgcccaaaag caacagagta tgactgttgc
     3661 caagctcgaa gaaaccggag gaacgagcct ccccgagaca ctcaagtggc tcgaaaagat
     3721 caaactctat ggcgatttcc gctatcgtta tgaaatggtc gatgaggatg gttcggacaa
     3781 ccgcaatcgc aatcgcattc gagcgcggct gggtctgact gccaaaatca atgacgaaat
     3841 cgataccggt ttccggttgg caacctccga actctgggga gatgacgagg actcgggcga
     3901 tcctgtatcg accaatcaga cactggatga cgccttttcc aaaaagagca tctggctgga
     3961 cctggcctat tttaactggc atccggcagg cattcagggc ttcaatgtca tcggcggcaa
     4021 gatggaaaat ccattctacc gcgtcggcgg caatcagctt atctttgaca gtgacctgac
     4081 gcccgaaggg attgcgtttc aatatacgca cggctggacg gaacagacga gctggtttgt
     4141 caatggcggg ggcttttatg ttgacgaagt caagagcggc gccgatacca gtttgtgggg
     4201 cgttcagggc ggtatcaaac acacctttca ggacaaatcc tatctcgtgg gcggtggcag
     4261 tttatactgt ttcggaaata tccaaggcca tggggctttt gtcaccgtca atggttctga
     4321 taagtttttc ggcaacagta aaagcggcgg tttatacatc aatgactatg atttgactga
     4381 gcttttcggt gaatatggtt ttaagcttgc cgatactccg acagcggtat ttgccaacta
     4441 tgtcaaaaac actgtcgccg ccggcagtga gaatgccggc tggctgattg gcacaaaata
     4501 cggcaaatgc aaggaccccg gttcatggga actgtcctat gattaccgcg acctcaaggc
     4561 cgacgcagtt gtcggtgcgt tcagcgattc agactttatc ggcggcggca ccgacggaaa
     4621 ggggcatcgc tttagcgctg tatatcagct tgccaaaaat acgcaggccg ggctgacata
     4681 ttttctgaat gaaaaaggtc atgatgatca tgattataac cggcttcaat tggacctgat
     4741 gtttaagttc tgaatatttc taatcaaaca gcaacacaac gctaactaaa cgctaacaat
     4801 tgggatgtat tatattacag gaaaggaatg gacttatgaa aacgttaata gcacaatttt
     4861 tgaccattgg atgtgtgttg gttggatccg tcgcggctgc cgaaaagctg caaattgacg
     4921 gctctacaac ggttggcccc atcggcgatg catttgctga agtatttaag gacaaggcag
     4981 ctgtaactgt cagcaaaacc gggtccggcg atggcgcggc agcactgatg gaagggcgct
     5041 gtgatatcgc tatgatgagc cgatttatga aaaacgcgga attcaaagcg gcggttgaca
     5101 aaggcatcat gccggtggct catgctattg caatggacgg tgtatgtgtg gtggtaaacg
     5161 agtctaatcc cgttaccggt ttgacaaccg aacagatccg ttctatctac aagggcgaga
     5221 ttaccaattg gaagcaggtc ggcggtccgg ataaggcaat tgtggtcatc agccgcgata
     5281 cttccagcgg aacatacgag acgtttgaag aactggtcat gcacaaagag aaaatggggc
     5341 cggctacgga gtacgtcaat tccaatccgc aggctcatgc ccgcgtcaaa agcaccgaag
     5401 gagcgatcgg atatgtcggt ctgggatttg tggaccgtta tgtcaaagcg ataaaagtgg
     5461 acggtgttat gcccgatcgc aaaacaattg ccagagggac atatcccctc agccggcctc
     5521 tgtttctttt taccaatggt tatccgaaac tggggtccat tgtgtatcag ttctgcacgt
     5581 ttcatctgac tgaagaaggg caggaaatca ttgaagccaa aggatttgta ccggttacgg
     5641 cctattaatg aaagattcct ccacacaatc tgttcaaaag cagcaggcgg gcgtatttga
     5701 gtttgctcgc ccgctgtgtc ttacagcgac agaggatttt cgcggtttta tcgctgctca
     5761 tctcggacaa ggacttttgt ttgtaacggc ctcgatttcc gttctggcgg tctttttaat
     5821 cttttttttt gtgattggcc aatcgcttcc cttctttttg aatagaggat tggcggaaag
     5881 tgtgccgcgg ataaaggaat tcttgacgag tactcaatgg tatccgcaaa ggcaggccgg
     5941 tccggagtat ggtgccaggg ccattattat cggctctctg tatgtaaccg tctgttcgct
     6001 gatgctggcg gtgccgattg gtctgctggc agctattttt ttaagcgata tcgccccctt
     6061 tggacttcgg caattctgca agccggtcat cgagattctg gcagcgattc catcggtggc
     6121 atacggcgtt ttcgctgtga tggtgctcgc tccatggatg caaaatacac tgggattttc
     6181 aagcggtacc aatgccctga atgcctcgtt gattttatct gtaatggcgt tgcctacgat
     6241 tattagtgta gccgaagatg ccttgtctgc ggcaggccgg gacatccgcg aggcctcgta
     6301 cgcactgggg gctacccgtg ccgagacgat gctcctggtt gtcctgcccg ctgcgcacag
     6361 cggaatcgtc gccgcgatta tactgggtat gatgcgggcg gttggagaga cgatggtcgt
     6421 ctggatggcc tccggcaatg ccagccgtat tcccagtccc tggtgggatt tgtcgcagtc
     6481 ggtgcggacc ctgactgcca ctatcgccgg cgaactcggc gaagtgccca aaaatacaga
     6541 ccattatcat gtgctgtttg ccatcgggct gttactgttg gttttcacct tcggcctcaa
     6601 tatgtgcagc gagtatttta tgggtcaatt tagaaaaatc acggggagac gctgaatgcg
     6661 gactgggatg cggcctgttt ttgactgtgt atttacgctt ctgtcggcgg catccgttgc
     6721 atttctaatt gcggccttgg ggatatttct gattcccctg tgcggcaagg gaatgaaggc
     6781 cgttgtgttt cgcgggacag tcgaattccg caaaatgcag tcagaccgct tcaatcgacg
     6841 gaatgaagcg acactgcaaa acgaaatcct ccagactcag caggtacggg atgaaatcta
     6901 cgcccttgtt gatcacttca gtcgcggttt ggacacacag actctggcag accaggcaaa
     6961 agatctgtat cgcaagtatg gcgacgagct gcgcagccgt gatctttcgc gccagcagtt
     7021 tgctgattta cgcgctgcgg ccaaagatat tcgggaccat ctgctggccg cttttgacag
     7081 cactgacaaa gctgttgtcg aggagcattt ggatgcgatt gagggcttga ggcatcagcc
     7141 cggctttgag aaaacccctg tcgaaacaat gttccggctt gcagatgatt ttcgtgaaat
     7201 gattcagaag gtggacctgt cccgccgcga ggaatacgca gaggaactgg ccgccgtcaa
     7261 taaagaattg tttgctctgt tcggcccgcg tcccgggcag cagcgggtct tggcgatgga
     7321 aagctacggc gcgacccgat gggatgtggc ccaaaaacac ctgcgttact taatttggca
     7381 ggaaaaatgg gtaccttctc cggacggcca aaccctgcag aaggttctca ttcgcagaga
     7441 tgatcccgaa aaaggatctt ttgccaaaac ggaattagcc ccgctgtttg ggctgattga
     7501 aaaaaacctt gctgcagcac tgaatccgcg gctaacgttt tattggcagt attttatcga
     7561 cgacagcaca ccggggcatt acttcggcgg ggtagggccg gagctgctgg gtacgctgat
     7621 gctgacgctg ctggcgatgt gttttgccgt gccgctgggt ataatttcgg ctgcttatct
     7681 ggttgaatat gcaggagata atcttgccgt acggatgatt cgtatctgca tcaatacctt
     7741 ggccggtgta cccagcatcg tttatgggct gtttggactg gcgtttttcg ttcattacat
     7801 caccaaacag ccgtgtatat tatcggcctc gctaaccttg gcacttctgg ttctgccggt
     7861 gattatccgg gctgctgaag aggccatccg cgctgtgccg cagagctatc gtgaagcggc
     7921 agtagggctg ggggctggca agttccgcac ctttgcgacc gttacgctgc cggcggccgg
     7981 tccgggaatc ctgacgggca tcattttgag tgtaagccgc gccgcggggg aaaccgcccc
     8041 cattttattt gccggggcgg tggcgctggg tccggttcct aatttcaccg gaaaatggtg
     8101 gtttcagccc acccgtgcac tgtcctatgg atgttatgat atcaccgtcg gcgacaaact
     8161 ggcggcgatg gtcccccaca accagttcgg gatggttatg acgctgatta ttttagtagt
     8221 atgcctgaat attactgcta tcctgattcg aatgcggctg agtaaaaaac ttaggggtgg
     8281 ataaaatgcc gattaaggag acagcaaaaa tccaaaatat ggatgtaaat ctaaaaatgg
     8341 acttaatctg cacagaagca gtagggcgaa tgtccggaat caatattgct gatgctgcga
     8401 aaaatatacc ctcagataat catattattc ccgagaaagc cattatccgg tcccgtgatt
     8461 ttacattcta ttacggccaa aagacggggg tagaaaagat aacgatggat attgaaatgg
     8521 gatccgtcac cgctatcatc ggccccagcg gctgcggaaa aagcaccttt ctccggagca
     8581 tcaatcgcat caacgacctg attccccaca cccatacgga aggccttctc gaggttggcg
     8641 gacagaatgt ctatgccaaa acgaccaact tggtcaatct tcggcagcag gtggggatgg
     8701 tctttcagag acccaatcct tttgccaaaa gtatctacga taacgtcgcc tatggtgccc
     8761 gccttttggg ggtacggaag aaagcggatt tggacgaaat tgtggaaaaa tgcctatgcg
     8821 atgctgcttt atgggatgaa gttaaagaca acctgcatca atcggccttg gccttgtcag
     8881 gaggccagca gcagcgtctt tgcattgccc gcacgctggc aacccggccg aaaattatct
     8941 tgatggatga accctgttcg gcactggatc cgctggctac ggcacgtatt gagaatttga
     9001 tcgccaagct caagggcacc tatacgatcg ttattgttac gcataatatg cagcaggccg
     9061 cacgcgtcag cgaccgaacg gcctttttct gtctgggcag actgattgaa tacgacatga
     9121 cggataagtt attcaccaat ccgcagcaaa aagcaacgga ggattatatt actgggaggt
     9181 ttggttaata tgtcccaaca cctgcagcat gaaatagaga atctgaaaaa aagcatcctc
     9241 agtctcggtg ctcggattga aacagccgtt cgtgaggcaa cgctggctat cgaacagcgg
     9301 gacggcatac tggcacagaa aatcattgat tacgatgatg tcatcgatca gcaggaggtg
     9361 gagatcgaag aggaatgcct aaagacactc gctttgcacc agccggtagc gattgacctg
     9421 cgatttatca ttactgtcct gaaaatcaac aacgacctgg agcgaatcgg cgatttggct
     9481 gtcaatattg ccgagcgtgc ggtttttctg gctgggcggc ctaaaccaga catttcgttt
     9541 gattttgtcg ggatggcttc tgcagcgcag atcatgctga aaaatagttt ggacgcactg
     9601 gtaaacctca gcgtgccgct tgctcaggaa gtgtgtgcgg gtgacaatac aatcgatgcg
     9661 atgaaccgaa aaatgtatgt taaaatccaa caggcgattt tggcccatcc cgaacaaata
     9721 gaatctttaa tccacatgct ctctgtttcg cgccaccttg aacggatcgc cgaccacgca
     9781 acgaatattg ctgaagacgt catttatatg attgaaggac gaatcgtccg gcatcgaatc
     9841 gaaaaaaaca tacttttaag ctaatgcgct gtgtcctaag caaaaatgac cgtcacgaag
     9901 acgcgggagg gatgggatgc agaaactgaa cgtgtcatgc tccggtcgtg atggagattt
     9961 ggccgtacct tcgaaaaagt ccattgatta agttcagtgc tctctgtccc ttgcaaaccg
    10021 taaaatgcct cgaatttaaa tccattttta ggctgtcatc gcgggggata atggcattaa
    10081 ggacaccaca actcgatttc aactgacttt atgtgaaaaa cgattccata ttgccctgtt
    10141 tgccgtcagc agaaagcggg acagcccccc gcagagggat aaggggctgt ggcggcagat
    10201 ggcgactcta tctgtccgat gctgccgatg ggggcagact ccctatgagg g
//
LOCUS       DAOTBO010000096        10097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5669_length_10097_cov_5.316471, whole genome shotgun sequence.
ACCESSION   DAOTBO010000096 DAOTBO010000000
VERSION     DAOTBO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5669_length_10097_cov_5.316471"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLE88_10185"
     CDS             <1..385
                     /locus_tag="PLE88_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76872.1"
                     /translation="PAVFEQQRQAFAQVAEQLQEKAARDEKVAEELRGIYEKTAAAAE
                     TNAQMCDHICKLSGTLSKIDEDTINQTQWIQQMSRSFSASERFLKYTFEKQQTRFYWL
                     FGISLGICFFAMTALIICFVLLLQR"
     gene            416..1393
                     /gene="trpS"
                     /locus_tag="PLE88_10190"
     CDS             416..1393
                     /gene="trpS"
                     /locus_tag="PLE88_10190"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOM76873.1"
                     /translation="MRVLSGIQPSGKLHIGNYFGAMRQHLELQAQNEGFYFIADYHAL
                     TSNPRPDALMQRTLDVAMDYLALGLDPVKTVFWRQSDVPEVTELAWILSCVTPMGLLQ
                     RCTSFKDKVAQGLSPVHGLFAYPVLQAADILMFDSNLVPVGADQKQHIEVTRDIAEKF
                     NAMYGQTFVIPQEHILQEVAVVPGIDGRKMSKSYNNTIEIFEPESSVKKKVMQIVTDS
                     TPVEEPKNPDTCNVFALLKLVASEAETAEWKDKYKTGGVGYGTVKKRVVELLHDYFHP
                     FRQKRQELEKNRDYVEQVLSDGARRARAIAAQTMRRVRDAVGLGEKRNG"
     gene            1386..1628
                     /locus_tag="PLE88_10195"
     CDS             1386..1628
                     /locus_tag="PLE88_10195"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOM76874.1"
                     /translation="MADKQKLVTVACYSNSFDASLAKLTLDKAGIDCVILGEPLAINI
                     TYGIPSVAVELQVRQEDEHRAKMILQEDIEQQKGCQ"
     gene            1625..2674
                     /locus_tag="PLE88_10200"
     CDS             1625..2674
                     /locus_tag="PLE88_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOM76875.1"
                     /translation="MSEYRVNLDTFAGPLDLLLYLVRKEEVDIYDIPIARITQQYVEY
                     IEMLKNLDIDLAGDFLVMAATLMEIKSAMLLPKTELNQQDEAAVGDPRAELVRQLLEY
                     KKYKDAANLLEQAGQQRQLRFTRSNYILEKIKPTQEPELDLDQISIWTLLEAFDAILK
                     ATGNINTRYDHIKDDTPIDLYQIELLHRLQAEGPMTLQKVFEGKNNRLAMIGLFLAML
                     ELMRNQLIWVEQPDSTGTIYLKSLTDEPAEQAVQKALYAASKIRDQKPQDEPVDTFQS
                     SDESSGQANNPDSDIDDIDDEFSAELSAIKISSETEEKPRISILELPAQQANGGLPQS
                     ANANKSDESVSPKAE"
     gene            2694..3611
                     /locus_tag="PLE88_10205"
     CDS             2694..3611
                     /locus_tag="PLE88_10205"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOM76876.1"
                     /translation="MIGLTHFLMLMVLSSGTPEMRPQELNSVTIRLWDATPPGAKGNQ
                     EKDVPTLTLYKSSATKTEGAAVVICPGGGYAGLAMDHEGRQIAEWFNRQGITAFILQY
                     RLPANGYRHPVPLLDVLRAIRLVRYNAAQWNIESNKIGILGFSAGGHLASTAGTHFDK
                     SIEMEGYKPDVIDAVSAHPDFMVLVYPVISMEDGITHRGSKNNLLGPNPSPELIELLS
                     NDKQVTPQTPPTFLVHADDDRGVVAENSICFYQALRKHHVPAEMHIYLKGGHGFGMRP
                     SAGPASQWPDECLKWLRQIHMVSAVDDER"
     gene            3601..4302
                     /gene="deoC"
                     /locus_tag="PLE88_10210"
     CDS             3601..4302
                     /gene="deoC"
                     /locus_tag="PLE88_10210"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOM76877.1"
                     /translation="MKDRVLFLIPQTRKDLARYFDHTLLDCMAGEQDIRRICKEAIQY
                     GFYAVCVQPRWVKLCADILHGSDVKVVSVAGFPFGTNLPKVKAYEAEAAIMDGADEVD
                     MVADLASIIAGDSAYLLREFNSVLAVCRRMKPAVVLKAIIESAALSEEQIRFVCGIAQ
                     DAGMDYIKTSTGFHKAGGARIKDVHLMASSAPQCRIKAAGGIKTAQEALAFIEAGAVR
                     LGSSSSVQILEQFNP"
     gene            4859..5743
                     /locus_tag="PLE88_10215"
     CDS             4859..5743
                     /locus_tag="PLE88_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOM76878.1"
                     /translation="MKSVSAFTANMSRSYYMKALEQIQHYIKDGHTYQINFSQRFETE
                     FFACPWDLFHWQNSCNPNPYAAYLTLDDGAIVCASPELFIKVCGQTIITKPVKGTRPR
                     NLCLPDQTGENQRYCDELLNSDKDKAELAMIVDLERNDLARICRPGTRRVICPRAIEA
                     FPTVYHTSAVIEGRLASKPDPRRLNEILKAVFPGGSITGAPKIRSMEIIDQLEPTGRG
                     IYTGSIGWIGINYDMCLNIAIRTILVHRQKAYVYAGGGIVADSIPQTEWEETLTKAEA
                     LLAGINAVAGMSKQHVHF"
     gene            complement(5759..6520)
                     /locus_tag="PLE88_10220"
     CDS             complement(5759..6520)
                     /locus_tag="PLE88_10220"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOM76879.1"
                     /translation="MNLAPASCKEVLYLDERKDFLLQAIQEQSPVIASYLFDGKWHLL
                     EMKTIGFSEDFITFKAQNPSENLKAEQPVGICIHQGYFKYLFDTAVQAVSSEGYMPLI
                     KMDLPDRVERIQRRMYHRQPVPASLKVKVLFWHRGYLEDTDERPEELYWEGRLLNLSA
                     GGAQFEIDAKEKNNFRAGQLLGVQFTPMSYQKPFLLESHVRYLKNQPDQTSFRIGVEF
                     LGLEASREGREVLNRLLEVIDIYRTMNKAAQSVSA"
     gene            6813..8156
                     /locus_tag="PLE88_10225"
     CDS             6813..8156
                     /locus_tag="PLE88_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76880.1"
                     /translation="MDIEKVKRYSLAEVVLLGIFVIGLIIANVIVRNRSRIVLSEPIV
                     LTGSGLSVSMPANPGWERTDVWRYEMDNSMTLIGRFRSRDADIEVRWRYNLHPPEVLP
                     DKLLQRRAAEANSALQEIEYLNEPVKMAFTALSGVSSLNSSAYMGATILDFGRSLELQ
                     VVSRTMDDYYAEHIFRSLAAGIQYKKPAALNEGLALLESFLMALGQKSNPIQPEAFLI
                     RDELGRNLGYFRCYENNQKLHQMRVWQYENRGVRYESSLWLNPSEKNYLWKTILKYPG
                     MTAPKTYDIRADGAGKLQVECNIEKPKISAASFFILPEILLPEYAAVLLDSTYNDVMV
                     DVLAPTGQVVPVHVEKIQKEQATAKSQNAAFVVRLDFLHHPNSFEELFFDPEGRLIGK
                     FEQQPRQRGRIWIAATLQQIESIFGEDFPMPQDQIATVNKLINLDSGMILKRRVK"
     gene            8153..9151
                     /locus_tag="PLE88_10230"
     CDS             8153..9151
                     /locus_tag="PLE88_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM76881.1"
                     /translation="MTETIYDCIIVGAGPAGLGAGLYTARDRQKTLLIDKFYPGGQIN
                     NTDRIENYPGYENISGPDLIMKMVNQVKNFGAEIKTSTEVKTLNRRPDGLIAVASAKH
                     TFLGRVVILSPGSSYRALGVPGEDQFRQAGTGVSYCGTCDAPFFKDKTVVAVGGGNTA
                     VEDTLHLAKYAKKVYMVHRRQEFRATKVLVEELLAYVSKPNSNLELRLDTVMTAIEGT
                     GKVEKAILKNVKTGQTEELQCDGVFIFVGMVPNTQFLKGFVDLTEQGFIRCDPAYLRT
                     RVPGVFAAGDCRCGAAMQLVTAVADGVNAAMWSKHYLRDAAWWNQPFHEALTPGEW"
     gene            complement(9167..9586)
                     /gene="nikR"
                     /locus_tag="PLE88_10235"
     CDS             complement(9167..9586)
                     /gene="nikR"
                     /locus_tag="PLE88_10235"
                     /inference="COORDINATES: protein motif:HMM:NF002169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOM76882.1"
                     /translation="MSTIERVGVSLEKDLLERFDALISKQGYTNRSEAIRDLIRLRLS
                     EEQLANPNIEAAAGVFLVYDHHAAKVADKLLDLQHGHFLEIIASTHIHLDHHNCLEVI
                     ILKGKVSHIEQLASRMGSLKGVKLSRIALMTPVGKIA"
     gene            9825..>10097
                     /locus_tag="PLE88_10240"
     CDS             9825..>10097
                     /locus_tag="PLE88_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76883.1"
                     /translation="MKSIKSDMLLLVLLTGFVWADPSEHMQQRGALTDGTVSLESSGL
                     DFAFSLTSIYQANTKGGISTHRRQGRWSGSYDLEMNADLERLLDIQG"
BASE COUNT         2738 a         2229 c         2513 g         2617 t
ORIGIN      
        1 gccggctgtt ttcgaacagc agcggcaagc attcgctcag gttgcagagc agcttcagga
       61 aaaggcggcg cgtgatgaaa aggttgctga ggagctgagg ggaatttacg agaaaactgc
      121 ggcggcagcg gaaacaaatg cgcaaatgtg tgaccatatc tgcaaattgt cagggacact
      181 cagcaaaatt gatgaagata caatcaatca gacccagtgg attcagcaaa tgagccggtc
      241 tttttctgcc agcgagcgtt tcttgaaata tacattcgaa aaacagcaga cccgttttta
      301 ttggctgttt gggatttctt tgggtatttg tttttttgcg atgacagcac ttataatctg
      361 ctttgttttg cttttacaaa ggtaaattta agaatctgat atataggagg cgatattgcg
      421 agtactttca gggattcagc catcaggcaa gcttcatatc ggaaattatt tcggggcaat
      481 gcggcagcat cttgagctgc aggcccaaaa tgaaggtttt tattttatcg ctgattatca
      541 tgcattgaca tcgaatccgc gtcctgatgc cctcatgcag cgaacgcttg atgtagcaat
      601 ggattatttg gctttgggat tggatccagt caagacagtt ttttggcgtc agtcagatgt
      661 gccggaagta acggaattgg catggatact gtcctgtgtg acccctatgg gactgcttca
      721 gcgctgtaca agttttaaag ataaggttgc gcagggatta agtccggtac atggtttatt
      781 tgcctatccg gttctgcagg cagccgatat tttaatgttt gacagcaacc tagttccagt
      841 cggagccgac caaaagcagc atattgaagt aacccgcgat attgccgaga aattcaatgc
      901 aatgtacggc cagacttttg ttattcctca ggagcatatc cttcaggaag tggcggttgt
      961 accgggcatt gacggtcgaa aaatgagtaa aagctacaat aatacaatcg aaatttttga
     1021 gccggaaagc agcgtcaaaa agaaggtgat gcagattgta accgattcta caccggttga
     1081 agaacccaag aatcctgata cctgcaatgt ctttgccttg ctgaagcttg tggcatcaga
     1141 agcggaaaca gctgaatgga aggataagta taaaaccggc ggtgttggtt atggaacagt
     1201 caaaaagcgt gtagttgagc tgcttcatga ctatttccat ccctttcggc agaaacgtca
     1261 agaattagaa aagaatcggg actatgtgga acaggtttta agcgatggag cccgccgggc
     1321 ccgtgccata gctgcacaaa cgatgcgtcg ggtaagagat gctgtcgggt taggagaaaa
     1381 gcgaaatggc tgataaacag aaattggtaa ccgtcgcctg ctattcaaac agttttgatg
     1441 cctcacttgc taagctgact ctcgacaaag cgggtattga ttgtgttatt ttgggggagc
     1501 cgctggcaat aaatataacc tatggcattc cctctgtggc cgttgaactg caggttcgcc
     1561 aagaagatga gcacagagca aagatgatcc ttcaggaaga tatagaacag cagaagggtt
     1621 gtcaatgagt gaatatcgtg tcaatctgga tacctttgcc gggccgctgg atctgctttt
     1681 gtatttggtg cgcaaagagg aggttgatat ttacgatatt cctattgccc gcattaccca
     1741 gcagtatgtt gaatacatcg agatgctcaa gaacttagac atcgatttgg ccggggactt
     1801 tctggtgatg gcggcaacgc tgatggagat taaatcggcg atgctgctgc cgaaaacgga
     1861 actgaatcaa caggatgaag cagctgtcgg ggatccccgt gccgaactgg tgcgtcagct
     1921 tctcgagtat aaaaaatata aagatgcagc caatctgctt gagcaagctg gccagcagcg
     1981 gcagctgcgg tttacccgtt cgaattatat actcgaaaaa atcaaaccga cgcaggagcc
     2041 tgaactggac ttggaccaaa tcagtatctg gacgcttctg gaggcatttg acgcaatttt
     2101 aaaggccacg ggaaatatca atacccgcta cgaccatatc aaagatgata cgccgattga
     2161 tttgtaccaa atcgaactgc tgcatcggct ccaagcagag ggtcctatga cgctgcagaa
     2221 ggtctttgaa ggcaaaaata accgacttgc gatgatcggg ctgtttttgg caatgctgga
     2281 gctgatgagg aatcagttga tatgggtcga gcagccggac agcacgggta ctatatattt
     2341 gaaatccctg acggatgagc cggctgagca ggcggtacaa aaagctctct atgcggcgtc
     2401 caaaatccgt gaccaaaaac cgcaagatga gcctgttgac acttttcaat cttctgacga
     2461 atcctcgggg caggcaaaca atccagattc cgacatagat gatattgatg atgaattttc
     2521 agccgaattg tctgccatca agatttcttc cgaaacagaa gaaaaaccgc gtatttctat
     2581 tttagagctt cctgcccagc aggcaaatgg cggtttacca caatcggcca atgcgaataa
     2641 atcggacgaa tctgtttccc caaaagcaga gtaactcatc ggattaaaaa actatgattg
     2701 gactaacgca ctttctgatg ctaatggtac tgagcagcgg aacaccagaa atgagaccgc
     2761 aggaattgaa tagtgtaact attcgcttgt gggatgcgac ccctcccgga gcaaagggaa
     2821 accaggaaaa ggatgttccg acgcttaccc tatataaatc ctctgccaca aagacagaag
     2881 gcgcagccgt tgttatttgc ccgggcggcg gttatgccgg tcttgcgatg gaccatgaag
     2941 gccgtcaaat tgctgaatgg tttaatcggc agggtattac ggcatttatc cttcaatatc
     3001 gcctgcctgc caatggctat cggcaccctg ttcctctgct ggatgtgctg cgggctattc
     3061 gactggtaag gtacaatgca gctcaatgga atattgaatc gaacaagatc ggaattctcg
     3121 gtttttctgc cgggggacat ctggcctcaa ccgctggcac tcattttgac aagagtatag
     3181 aaatggaagg atataaacct gatgtgattg atgcggtttc agcacatccg gattttatgg
     3241 ttcttgtgta tccagtaatc tccatggaag atgggataac ccacaggggt tcaaaaaaca
     3301 atcttctggg ccccaatcca tccccggagc tgattgaact tctttcgaat gataagcagg
     3361 ttactccgca gactccgccg acttttttgg ttcatgccga cgatgataga ggcgtagttg
     3421 cagaaaatag tatttgtttt taccaagcat tgcgaaaaca ccacgtgcca gcggaaatgc
     3481 atatatatct caaaggagga catggttttg gaatgcggcc aagcgctgga cctgcttcac
     3541 aatggccgga tgaatgctta aaatggctgc ggcagattca tatggtgtct gccgtagacg
     3601 atgaaagata gggtactgtt tttgattccg cagacacgaa aagaccttgc ccgttatttt
     3661 gaccatacct tgctggactg tatggcaggg gagcaggata tccgacggat ttgcaaagag
     3721 gccattcaat atggatttta cgccgtatgc gtgcagccgc gatgggtcaa actgtgtgcg
     3781 gatattttgc atggaagcga tgtaaaggtt gtctcggttg caggctttcc gtttgggact
     3841 aacctgccca aagtaaaagc ctatgaagca gaagctgcta taatggatgg tgcagatgag
     3901 gtggatatgg tggcagattt ggcgtctatt atagcgggcg attctgccta tctgcttcga
     3961 gagtttaaca gcgtgctggc agtctgccgc aggatgaagc cggcggttgt cttaaaggcg
     4021 attattgagt ctgctgcttt gagtgaggaa cagatacgtt ttgtctgcgg cattgctcaa
     4081 gatgcaggta tggattacat aaagaccagt accggttttc acaaagccgg cggagcacgg
     4141 attaaggatg tgcatctgat ggcctcatct gctccccagt gccggatcaa agctgccggc
     4201 ggaattaaaa cagcccaaga ggcactggct ttcatcgaag caggagcagt ccgtcttggc
     4261 tcgtcttcat ctgtacagat acttgaacaa ttcaatccct gacatgaaaa actcagcaca
     4321 gcggcctata ttctgcaagc cctgttttta tcccgtatcg acacatgccg gcatagcggc
     4381 aatcagcaag gcagttgccc aaaaacgctt tcctgctgtt cttggggcga atgcatcggt
     4441 tgcaggatgg agcatatttg cagccgaacc aaccgaaatc ttttcatttt gcctcggcga
     4501 tgatcatcct tttgaaaaac tgcaatgtct cctttcaaaa tatcaatgct gtccttcagc
     4561 aaaaccattt gctgcgggtg ttttctgcgg gggatggatt ggatatttcg gatatgagct
     4621 gaatcggttt atcgaacgat tgcccgccag atccagagat gacttggata ttcccattat
     4681 ccagctggga ttttatgata aagcaatttt atataatcat aacaccgcac gcgttttctt
     4741 attagcgctg gactgccaaa ggccgccaca gcagccggtc gaacagaaaa ttgaagtttt
     4801 gaaaagatgg ctctgtgagg cggagaaaat aagcgtctgt cggccagacg cagaatctat
     4861 gaagtctgta tctgcattta ctgctaatat gagtcggtca tattatatga aggcgctgga
     4921 gcagattcag cactatatta aagacggcca tacctatcag attaactttt cccagcggtt
     4981 tgagaccgaa ttttttgcct gtccgtggga tctgttccat tggcagaata gctgcaatcc
     5041 aaatccctat gcggcctatc tgacattgga cgatggggcg atcgtgtgcg cctcgccgga
     5101 attatttata aaagtctgcg gtcaaacgat tataaccaaa ccagtcaaag gtacgcgtcc
     5161 gcgaaatctc tgtttacctg atcaaaccgg agagaatcag cgatactgtg atgaacttct
     5221 caacagcgac aaagacaagg ccgaattggc aatgatagtg gatttagaaa gaaatgattt
     5281 ggctcgtatc tgtcggcccg gcacacgacg ggtaatctgc cccagagcca tcgaagcgtt
     5341 tccaaccgtt tatcatactt cggcagtaat agaaggacgc ctcgcgtcaa aacccgaccc
     5401 caggcgactt aatgaaatct tgaaagcggt cttccccggc ggctcgataa ccggcgcacc
     5461 gaagattcgt tcgatggaaa ttattgatca gctggaacca actggccgcg gtatttatac
     5521 cggaagcatc ggatggattg ggatcaatta tgatatgtgc ctcaatattg ctatacgcac
     5581 catattggtt caccggcaaa aagcgtatgt atatgcaggc ggcggaattg tggccgattc
     5641 cataccccag accgaatggg aggaaacgct caccaaagca gaagcgcttt tggcaggtat
     5701 caatgctgtt gccggcatgt caaaacagca tgtgcatttt tgaaaccggc acagacagtc
     5761 aggcagagac agattgagct gctttgttca ttgttctgta aatgtcaata acttccagca
     5821 gccgatttaa tacttcgcgt ccttcgcggc tggcttccaa gcccaagaat tctaccccaa
     5881 tccgaaaact tgtctgatca ggctggtttt tcaaatatcg cacatgtgat tcaagcagaa
     5941 agggtttctg ataactcatc ggagtgaact gaactccaag aagctgtcct gccctgaaat
     6001 tatttttttc ttttgcatca atttcaaatt gcgcaccgcc agcagacaga ttcagcagcc
     6061 ggccttccca gtacagttct tcgggccgct cgtcggtatc ctctaagtag ccgcgatgcc
     6121 aaaaaaggac tttgaccttt aatgatgcag gaacgggctg acgatgatac atacggcgct
     6181 gaatccgctc aacacggtcc ggcaaatcca tttttatcag gggcatatat ccttcgctgc
     6241 tgactgcctg cacagccgtg tcaaataagt atttaaaata tccctgatga atgcatatgc
     6301 cgacaggttg ttcggccttt aggttttcgg atggattttg cgccttaaat gtaataaaat
     6361 cttcagaaaa ccctattgtt ttcatttcca gcaagtgcca cttgccgtcg aatagatagc
     6421 tggcgataac cggtgattgc tcttgaatgg cctgaagcaa aaaatccttt ctctcatcta
     6481 aataaagaac ttctttgcag gatgccggcg ctaaattcat aaataagccc ttttttttat
     6541 cgcatatcta aacttttttg ggtatctctc ttcccataaa tcggctcatt tgagcagaac
     6601 cttgagggct tcccgaacag ttatacactt ccatggaata ccaacctgac tataaaaacg
     6661 gcttgttgca gttaagttct ttattcgcaa taagatgcta aactcctcta tcgactttgc
     6721 agccatcagc ggttagattt ttcatccgtt cgcatcagtt tttgcaacac gtcggttgaa
     6781 aaatcggata tttgaaggta tgatttgaaa ttatggatat agagaaagta aaacgctatt
     6841 cgctggccga ggtcgtcctg ttgggcattt ttgttatcgg actaataatc gccaatgtta
     6901 tcgtccgaaa ccgttcgcga attgttctat cggaaccgat cgtactgacc ggatcgggct
     6961 tgtctgtctc aatgcctgcc aatccgggtt gggagcgtac ggatgtctgg cgctacgaaa
     7021 tggacaattc gatgaccttg attgggcggt ttcgcagccg cgatgcagac atcgaggtcc
     7081 gctggcgtta taatcttcat ccccctgaag tgctgccaga caagttgctg caaagacggg
     7141 cagcagaagc caattccgcg ctgcaggaaa ttgaatactt aaacgagccg gttaaaatgg
     7201 cctttacggc tctcagtggt gtttcgagcc tcaacagcag tgcctatatg ggcgcaacta
     7261 tattggattt cggccgttcg cttgaactcc aggtagtttc ccgtacgatg gatgactact
     7321 acgcagaaca tatctttcgt tcgttggcag ccggcattca atataaaaag ccagccgcat
     7381 taaacgaagg gctggccctg cttgaatcat tccttatggc tttaggacag aagagcaatc
     7441 cgatacagcc ggaggcattt ctaattcggg atgagctggg aagaaacctc ggctattttc
     7501 gctgttatga gaataaccag aagctgcacc agatgcgggt ttggcagtat gaaaaccggg
     7561 gcgtaagata tgaatcatcc ttgtggctta atccctctga gaaaaactac ctttggaaaa
     7621 ccatcttgaa atatccgggg atgacggcac cgaaaacgta tgatatcagg gccgatgggg
     7681 cgggaaaact gcaggtagaa tgcaatatag aaaaacctaa aatatcggct gcatcctttt
     7741 ttattttgcc ggagattctc ctgcccgagt atgcagcagt gttgctggac agcacgtata
     7801 atgatgtaat ggtggatgtt cttgccccaa caggtcaggt tgtgccggtg catgtggaaa
     7861 aaattcaaaa agaacaggcg acggcaaaat cccagaatgc tgcatttgtt gtccgtctgg
     7921 atttcctgca tcatccgaac tctttcgagg agctgttttt tgacccggag ggcagactga
     7981 ttggaaaatt tgagcagcag ccgcgtcaga ggggtcgaat ttggattgcg gcaactctcc
     8041 agcagattga atctattttc ggcgaagatt ttcctatgcc gcaagaccag atagccacag
     8101 tcaataagct catcaatttg gattctggta tgattttaaa acggagagta aaatgacaga
     8161 aaccatttat gactgcatca ttgtaggggc aggtccggcc ggattaggag ccggtttata
     8221 tacagcacgt gaccggcaaa agacgctttt aatcgataag ttttatcccg gcggacaaat
     8281 caacaatacc gaccgtatcg aaaattaccc cggctatgaa aatatcagcg gccccgacct
     8341 gattatgaaa atggttaatc aggtcaaaaa cttcggggct gagataaaaa ccagcaccga
     8401 agttaaaaca ctcaaccgca gaccagacgg ccttattgca gttgcttctg ccaaacatac
     8461 ctttttagga cgcgttgtta tactttcgcc gggcagttcc tatcgtgctc taggcgtgcc
     8521 gggagaagat caattccgac aagccggtac aggcgtcagc tactgcggta cttgtgatgc
     8581 gccctttttt aaggataaaa ctgtcgtcgc cgtcggcgga ggcaatacag cggttgaaga
     8641 tacgctgcat ctggctaaat atgccaaaaa ggtctatatg gtccaccgtc ggcaggagtt
     8701 tcgagcgaca aaagtgctgg tcgaagaatt gctggcatat gtcagcaagc ctaacagcaa
     8761 tctcgaatta agattggata cggtgatgac ggctattgaa ggcactggaa aggtcgaaaa
     8821 agcgatattg aaaaatgtta agacagggca aaccgaggag cttcaatgcg acggcgtttt
     8881 tatctttgtt ggcatggtcc caaacaccca gttcctcaaa ggttttgtgg atttgactga
     8941 acagggattt atccgctgtg atccggccta tcttcgaacg cgggtgccgg gggtatttgc
     9001 ggcaggggac tgccgatgcg gtgctgcgat gcagctggta accgcggttg ccgatggcgt
     9061 taatgccgca atgtggtcca agcattatct tcgcgatgcg gcgtggtgga atcagccgtt
     9121 ccatgaagcc ctgacgccgg gcgaatggta aaaactcctc tgtctgtcag gcaattttgc
     9181 caacaggagt cattaaggca atccggctga gcttgacacc ttttaagctt cccatacggc
     9241 ttgccagctg ttcgatatgg ctgactttgc ctttgaggat aatgacttcc agacaattat
     9301 gatgatcaag atggatatgg gtagaggcga taatctctaa gaaatgacca tgctgcaggt
     9361 caagcagctt gtcagccact tttgctgcat gatggtcata aacaagaaaa actccggccg
     9421 cagcttcgat atttggattt gccagctgct cctcggatag acgcagccga ataaggtctc
     9481 ggatggcctc cgaacggttg gtatatccct gtttgctgat taacgcatcg aaccgctcca
     9541 atagatcttt ttccagcgaa accccgactc gttcaatagt gctcataaaa tcctcttgct
     9601 aagacaaaac ttttatccca tctgatacgc cgcagttagt tttctagaca gaagcattaa
     9661 attcagttga aaaaaaaggg gtttttataa attctgccca atctgaataa aatttagggc
     9721 aatttatttt tttttcaagt aattccttga cccagtagca caaataatat atttttatgc
     9781 gttagtagca caaataataa aattttatgc gcaaagataa gaaaatgaaa tccattaaat
     9841 cagacatgtt attgctggta ttgttgacag gatttgtatg ggccgatcct tccgaacata
     9901 tgcagcagcg gggtgcctta accgatggca ctgtatcatt ggaatccagc ggacttgatt
     9961 ttgcgttcag tttgaccagc atctatcagg ccaacaccaa ggggggaata agcactcacc
    10021 ggcggcaggg ccgctggagc ggaagttatg acttagaaat gaacgccgat ctggaacgcc
    10081 tgctggatat acagggc
//
LOCUS       DAOTBO010000097        10085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5677_length_10085_cov_5.745663, whole genome shotgun sequence.
ACCESSION   DAOTBO010000097 DAOTBO010000000
VERSION     DAOTBO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_10085_cov_5.745663"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLE88_10245"
     CDS             complement(<1..683)
                     /locus_tag="PLE88_10245"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOM76884.1"
                     /translation="MRFCIILGAMKAGTTSLFRCLAQHPQIAPCSVKEPSFASRYYDR
                     GRDWFLSLWKGQNLDNKILLTASTNCTKYPDFPESSWNILDYSQKFNAAIKFLYIMRD
                     PIARIESHYTYSFARWTSEPLEARIKPNGHLVNVSRYALQLDQYTEKFDRKNFLLLDF
                     DELRNNPAPVLAHICRFLEIDDRFQFANLGEVHNKSEGTRIVRPVERLYLKYPRIERA
                     AKKIPLGIR"
     gene            complement(692..1900)
                     /locus_tag="PLE88_10250"
     CDS             complement(692..1900)
                     /locus_tag="PLE88_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOM76885.1"
                     /translation="MGLAEHTTPASTHSGGGLSGPSRFLITAAAFVVVIAGMRAAAAV
                     IVPFLLSVFLAIISTPALFWLKNRGISGVWAILLVSGVILLAGLLVGTVLTASVADFT
                     QDLPMYAAKLNDEAAKLEQKWKVWMEKIHSDFSRHHHNNGQEENPSFSQTASDSIFNE
                     ASNSDNPQGLTQFLDTRLIIRLMRDLLGQLGSIAANGFMIYLTTVFILLEASILPGKI
                     KTVMKNNPKAFDNLASAAGDVKKYLALKTIMSLATGILIAIWLIVLGVKYPIVWGLIA
                     FLLNFVPNIGSIAAAIPACLLALVQIGPVSAGLAVLGYLAVNITIGNIIEPRVMGQRM
                     GLSTLVVFLSLVFWGWVLGPVGMLLSVPLTMTLKIVLQSHPDTCTLALLLGSQNPRSE
                     QTEKLSAKKR"
     gene            2108..3916
                     /locus_tag="PLE88_10255"
     CDS             2108..3916
                     /locus_tag="PLE88_10255"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOM76886.1"
                     /translation="MESSIKNSVGIVQTQKLRVVEADSPLQLVCGKTLGPIDVAYETY
                     GCLNEQKDNAILVCHALSGDAHAAGFNSPDDRKPGWWDIMIGPGKPIDTSKYFVICSN
                     VLGGCSGTTGPSDINPATGQPYGLTFPIITIEDMVKVQKLLLEKLNISHLLAVIGGSM
                     GGMQVLQWAVSYPEFADAVICIASTTRLNAQSIAFDAVGRNAILADPNFNDGQYHGHQ
                     LPARGLAIARMIGHITYLSEESMRYKFGRGLRDSSDYKYDFDSEFSVETYLDYQGQTF
                     VERFDADSYLYITKAMDYFDLMREYGSLRQAFANSAGRFLIVSFSSDWLFTPKQSEEI
                     VEALGAEGKDVTYCNIYSTYGHDAFLLESDKLGRIIQGFVDSTWHQRCSARRIETFES
                     PADLQEKMVDHPKRIRVDYELIDSLIESNSRILDLGCGDGELMSRLAQTKNACAEGVE
                     LDEDLVIHCIQRGLSVIHRDIERGLEMYPNEAFDYAVLSQTIQTLRDPQQVLLELHRV
                     ARRVIVSFPNFAHWRCRVQLMFKGIAPQTRQLPFRWYNSPNIHFLSLKDFEHFCAELD
                     IRIERKIPLGKSTLPLRLLPNLLAPQAVYVTSKYQS"
     gene            complement(3932..4663)
                     /locus_tag="PLE88_10260"
     CDS             complement(3932..4663)
                     /locus_tag="PLE88_10260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOM76887.1"
                     /translation="MRIPRFFCESIVDESILDLPESAHLCRVLRGKPGTRVELFDGKG
                     TLAAGIVSRIEKKQVYVRIQTIHQIRPRETGRIILAISYAKGQRFDWLVEKCTELGVD
                     HIAAVLFDRTVKLGSPTIAERIDKIAIAASKQCGRLFLPVISGPAGLRESLDFLRTMY
                     ANAALLFGDPHGQPLHQNQSDSAGRDFIVLIGPEGGFTETEIEVLQNAGALPVCLNPN
                     ILRIETAAAAFCAVLAAGRLSEPAQ"
     gene            complement(4857..5078)
                     /locus_tag="PLE88_10265"
     CDS             complement(4857..5078)
                     /locus_tag="PLE88_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76888.1"
                     /translation="MIKFDCIKCGHSYRVPDEYAGKRTRCKECSTINIIPTPEKEKVG
                     CGDSIAAYNSLLEELSKYEKQAPAIDSES"
     gene            complement(5359..5598)
                     /gene="rpmA"
                     /locus_tag="PLE88_10270"
     CDS             complement(5359..5598)
                     /gene="rpmA"
                     /locus_tag="PLE88_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOM76889.1"
                     /translation="MAHKKGQGSTRNGRDSNPKYRGVKLFGGQFAKAGAILVRQCGTK
                     FHPGFNVGMGKDFTLFAKTDGIVQYRGKRVDIDTN"
     gene            complement(5916..7586)
                     /locus_tag="PLE88_10275"
     CDS             complement(5916..7586)
                     /locus_tag="PLE88_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM76890.1"
                     /translation="MKAIPVLSAINTRHKAGWAVFLLTGILLVWGCGPSPEEAASAAD
                     KRIYGAIEKATKKAGLEQNDYYRLDDPNQREAVRAVLPQNSVLTLTQAAAMAVTYNRD
                     YLLERDNLFQLASGVTDIQHLYEIIPGGSIQEDYLRQDGQETVGTAGGLGLGLRQLLA
                     AGTQVGANLSLTCFDLLSGGDGSIKSGFASVFSATVTQPLLRGASRKAVLETLTQAER
                     DLLYQIRYFNRYRKTFLVTVLQTYYETLQFYDQMNNARRYYLTLAEIHRQTQKQAAVG
                     RVPLFEVEQTYQDQLDALNTYIDLRNDYGQMLDTLKTMLAIRPDTELVLDIQELEALK
                     AFDFAELNFSEEAAIQAALNQRLDLANAADRVLDAQRHVEIAAEMLGADLALIGTTSV
                     GSFGASPDDLRRTEDMYNLSLRMDLPLDRKAEQNRYRLTLAELMQNQRTHQGITDQVI
                     QEVRSAFRNLRTAHEQYRLALNAQELAQKRMHNTLRLQKVGRANMRDVLDAHEDYYEA
                     QENATDALVEYAVAALNLYLATDTLKVQPDGQLLKSADSFGNSMPNNN"
     gene            complement(7583..8701)
                     /locus_tag="PLE88_10280"
     CDS             complement(7583..8701)
                     /locus_tag="PLE88_10280"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM76891.1"
                     /translation="MNKTIPVFVWLAALGGVTVLFIQQGSRVDFNGLVIGQEQAITVA
                     ENGYIKALPVRLYEEVKKGDTLAVVQISTIGNDMYDTTLIDARRATAQAELERLKAEL
                     KAAEAQLEAEQIDRQRDIFDIQRRLAVDVERARISILEAKTELEPNRALLKDLELEVQ
                     IAEDLYKDNAIESYEVQKIKSQCEVTRKTVESYQQLLAQAEADYAQAQLRMDEFMQSA
                     ALPSAIAKILDPLAKAITVQEKVLEEIIRPTDTVILTAPFDGVVSSLFYKPGQAVMRD
                     IPIMTIVKPAPDYVAAWVPQEKIPLLHIDMPVQIVTRRTPQQAVTSRITHISPSLELL
                     PERLWQSPTLPEWGQVIIIPLQPGTKLVPNEIVGVKGL"
     gene            complement(8748..9449)
                     /locus_tag="PLE88_10285"
     CDS             complement(8748..9449)
                     /locus_tag="PLE88_10285"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOM76892.1"
                     /translation="MMNNILEIIENASAHSLNYSLQSVLLSLLLAFVLGQALAWLYYF
                     THSGLSYSRSFVQSLILITVIVAMVMSTIAGSFVVAVGLMGALSIIRFRNMIKDTRDI
                     AFIFCSLVIGMACGSQRYSIAVIGTVCIALIILYLHVSDFGTHTPHNAFLRFRFNGHF
                     GADHPIMRVLKKFCGNFTLISAQDGDFGNQDVEYAYQVMIKNVSKNEAMLAELQQIEG
                     VKNISLTVQEQLLEI"
     gene            complement(9439..>10085)
                     /locus_tag="PLE88_10290"
     CDS             complement(9439..>10085)
                     /locus_tag="PLE88_10290"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOM76893.1"
                     /translation="KPGNEYLISSLYLDTPTLTLCNETLQGKKNRFKLRIRGYDDNPL
                     SPVFLEIKRRACNVIVKSRVGVKKAELGDILAGRYIPPLTYAKDRDAFNQFMMYAAAL
                     SARPIVLVRYMRQAFEGDSDNRVRITFDRQLCFKTTCQPVVSVNGTGWTSVAMDFVVL
                     EIKFTSRYPLWLSEMVKIFDLKQSSMSKYVSSVQQSCQLGYCSPPAGSIWCYDE"
BASE COUNT         2520 a         2531 c         2358 g         2676 t
ORIGIN      
        1 ctgcggatgc ccagaggaat ctttttagcc gcccgctcaa tgcgcgggta ttttaaatag
       61 agtcgttcaa ccggccggac aatgcgggtc ccttcgcttt tgttgtgcac ttctccaaga
      121 ttggcaaact gaaaacggtc atcaatttcc aaaaacctgc agatgtgggc taagacagga
      181 gccggattat ttctcaactc atcaaaatcc agaaggagaa aattcttccg gtcaaatttt
      241 tccgtatatt gatcgagctg aagagcatag cgcgagacgt tgaccagatg gccattcggt
      301 ttgatgcggg cttcgagagg ttcgcttgtc cagcgggcaa aggaataggt gtaatgcgat
      361 tctatccggg cgatggggtc ccgcataatg taaagaaact taatagccgc attgaacttt
      421 tgagaataat ccagaatatt ccagcttgat tcgggaaaat cgggatattt ggtgcagttg
      481 gtcgaagctg tcagcaaaat cttattgtcc agattctgac ctttccacag tgataagaac
      541 cagtcccgtc ccctgtcata gtagcggctg gcgaaactcg gctccttgac actgcagggt
      601 gctatttgcg gatgctgggc taaacacctg aataggctgg ttgtgccagc tttcatcgcc
      661 cccagaataa tacaaaaacg cattgttacc cttatctctt tttggcagat agtttttcag
      721 tttgttctga ccgaggattc tgtgagccca gcagcagagc cagtgtgcaa gtgtccgggt
      781 ggctttgcag aacaatttta agcgtcatcg tcagcggcac tgacagcagc atccctacag
      841 gtccaagcac ccacccccag aagaccaatg acagaaagac caccagtgtc gacaatccca
      901 tccgctgtcc cattacacgc ggctcgatga tattgccgat ggtgatatta acagccagat
      961 accccaatac tgccagtcct gccgaaacag gaccgatctg caccagcgct aacagacaag
     1021 ccggtatggc cgcagcaata gagccgatgt tcggtacaaa gttcagcaaa aaagcaatca
     1081 gcccccagac aatcggatat ttaacaccca gcacgatcag ccatattgca atcaggatgc
     1141 cggtggccag actcatgatt gttttaagag ctaaatattt tttaacatcg ccggccgccg
     1201 acgccagatt gtcaaaggcc ttgggattgt ttttcatcac cgttttgatt ttaccgggca
     1261 ggattgacgc ttccagcaga ataaaaacgg ttgtcaggta tatcataaat ccgttggcgg
     1321 ctatgcttcc caactggccc agcaggtcac gcatcagccg gataatcagc cgcgtatcca
     1381 gaaattgtgt caatccctgc ggattgtcag aattggacgc ttcattaaat atgctgtcgg
     1441 aggcagtttg actaaaactg ggattttcct cctgtccatt gttgtgatga tgcctgctga
     1501 aatcggaatg gattttttcc atccagactt tccatttctg ttccaatttg gctgcttcat
     1561 cgtttagttt ggctgcatac atcggcaagt cttgtgtaaa atccgcaact gatgccgtca
     1621 aaacagttcc taccaaaagg ccagccaaca gaataacgcc agaaaccagc aagatagccc
     1681 atactcctga aattccccgg ttttttagcc aaaacagtgc cggcgtgctg ataatcgcca
     1741 gaaaaaccga taaaagaaac ggcacaataa cagctgctgc ggcacgcatt ccggcgataa
     1801 caaccacaaa tgccgctgcc gtaattaaaa atcggcttgg tcccgataat cccccgcccg
     1861 aatgagttga agcaggtgtt gtatgttctg ccagccccat aagcaatcct gttttttgca
     1921 ttaaggtaat tcagttatcc atcatacggg cagttcacac agacgtcaac tctttttcag
     1981 attaattgta tcgtaaaaaa gagccatttg cttggcgagc atagattatg gtattagcgg
     2041 tacagcgctc aaatttttgg tcttcaaaac ctctgttttt tttggaaaac ggcggtatac
     2101 tatggttatg gaaagcagca taaaaaattc ggtaggcatt gtccaaactc aaaaactgcg
     2161 tgtggttgaa gccgattcgc cgctgcagct ggtatgcggc aagacgctcg ggccgattga
     2221 tgtagcttat gaaacatacg gctgcctgaa tgagcaaaaa gacaatgcta ttttggtttg
     2281 tcatgcactc agcggcgatg cccatgctgc cggcttcaac agtcctgatg accgcaagcc
     2341 cggctggtgg gatattatga ttggccccgg caaaccgatc gatacatcca aatattttgt
     2401 tatctgctcc aatgtcttag gcggctgcag cggtacaaca gggccttcgg atatcaatcc
     2461 ggctaccggt cagccctacg gtttaacatt tccgataata accatcgaag atatggtaaa
     2521 agttcagaaa cttcttttgg aaaagctgaa tatatcccat cttttggcgg ttattggcgg
     2581 ttcaatgggg gggatgcagg ttctgcaatg ggctgtttcc tatccggaat ttgcagatgc
     2641 ggtaatatgt attgcttcta ctacgcggct caatgcccag tcgattgcat ttgacgccgt
     2701 tggccgcaat gcaatcttgg ccgacccgaa ttttaatgac ggtcaatatc acggacacca
     2761 actccctgca aggggattgg ctattgcccg gatgatcggc catattacct atctgtctga
     2821 agagtcgatg cgatacaagt tcggccgtgg tttacgggat tcgagcgatt ataaatatga
     2881 ttttgattcc gaattttcgg tagaaacata tttggattat cagggacaaa catttgtcga
     2941 gcggttcgat gctgacagct acttatatat taccaaagca atggactatt ttgacctgat
     3001 gcgggagtat ggttcactcc gtcaggcctt tgccaattct gccgggcggt ttctgattgt
     3061 cagtttttcc agcgactggc tttttacgcc taaacagtcc gaggaaattg tcgaggccct
     3121 tggagccgaa ggcaaagatg tcacttattg caatatctat tcaacctatg gccatgatgc
     3181 atttcttctt gaatcggaca agctggggcg gattatacag ggatttgtcg attcgacatg
     3241 gcaccagaga tgctcggccc gccggattga aacgttcgaa tccccggcag atctgcagga
     3301 gaaaatggtc gatcatccaa aacgcattcg ggtggattat gagctgattg attctctgat
     3361 tgagtcaaac agccgcatct tggatttggg ctgcggcgat ggggaactga tgagccgtct
     3421 ggcgcaaacc aaaaatgcct gtgccgaggg ggtagaactg gatgaagacc ttgtcattca
     3481 ctgcatccag cgaggactgt cggtgattca ccgggatatt gaaagaggtc ttgagatgta
     3541 ccccaatgaa gcgtttgatt atgccgtgct gtcgcagact atccaaacgc tgcgcgaccc
     3601 ccaacaggtg ctgctggaac tgcatcgtgt cgcacggcgg gtgattgtca gctttcccaa
     3661 ctttgcccac tggcgctgcc gtgtccagct gatgttcaaa ggcattgctc cccaaacccg
     3721 tcagcttccg ttccgatggt acaactcgcc caacatacac tttttgtcat tgaaggactt
     3781 tgaacacttc tgtgcggaac tggatattcg cattgagcgg aagattccgc tgggcaaaag
     3841 cacactaccg cttcggctgt tgcccaatct gctggctccc caagcggtct atgttaccag
     3901 caagtaccaa tcctgatgtg tgttcactga cctattgagc cggttcggat agacggccgg
     3961 cggcaagaac agcgcagaat gctgccgcag ccgtttcaat gcgtaatata ttgggattca
     4021 gacagaccgg cagggcaccg gcattttgaa gcacctcgat ttcggtttcc gtaaatccgc
     4081 cttccggacc gatcagcacg atgaagtccc gtccggctga atccgactgg ttctgatgta
     4141 acggctgccc gtgaggatcc ccgaataaca gagcagcatt tgcatacatt gtgcgtaaaa
     4201 aatcaagtga ttccctcagc ccagccggtc cgctaataac tggcaaaaag agccgtccgc
     4261 actgcttgga tgcagctata gcgattttat cgatacgctc cgcgatcgtt ggactgccca
     4321 gctttacggt tctgtcaaac aaaaccgctg caatatggtc caccccgagt tctgtgcatt
     4381 tttcaaccag ccagtcaaac cgctgacctt tggcgtaact aattgccaaa ataatgcgtc
     4441 cggtttcacg gggtcgaatc tgatgaatcg tttgtatcct gacataaact tgctttttct
     4501 cgatgcggct tacaatacct gcggccaatg tgcctttgcc atcgaaaagc tcaacccttg
     4561 ttcccggttt tccgcgcaat acgcggcaca ggtgagcaga ttcaggtaaa tccaagatag
     4621 attcatcgac aatagattca cagaaaaaac ggggtattcg catcacttat agtcccagct
     4681 gaaactctca gacagcaatt ttttcagcgc actatagccc aaaaagtgtt ttgtccatat
     4741 gaaaaaatga cttggtatta ctgtttcccc tctatgcccg ataacatatg gtttatttac
     4801 acaataaacc tttccccccg tattttaatc tattcgattg ctcacttcgg gaatgttcag
     4861 gactcactgt caattgctgg cgcctgcttt tcatatttcg acagttcttc cagcaggctg
     4921 ttataggccg caatgctgtc gccgcaaccc accttttctt tttcaggggt tgggataatg
     4981 ttgatggtac tgcattcctt gcagcgagtt cgtttccccg catactcatc ggggacacga
     5041 taactatgtc cgcatttaat gcaatcaaac ttaatcataa aagctcactt ttcttcggtt
     5101 tcttcggttt tctctctttt ttcgagtctt tccaaactcg tctctatgct tctctatata
     5161 ataatgccga aaaaacagaa attattactc aaattcttga agattttcgt taattttaca
     5221 aaagctgctt ttttaaaaat ccaaattctc caaattaact gtttttagag aatatctata
     5281 aaaacagccc tgacttttca tggacagggc tgaaagagac tgtaaatgtc ttcaaccgct
     5341 tagaatagtt aaggtatgtt agttggtatc tatatctacc cttttaccgc ggtattgcac
     5401 gatgccgtct gttttggcaa ataatgtgaa atcctttccc attccgacat taaaacccgg
     5461 atgaaacttt gtcccgcact gacggaccag aatcgctcct gctttggcaa attgtccgcc
     5521 gaacagcttt acccctcggt acttcggatt gctgtcacgc ccatttcgcg ttgaaccttg
     5581 tcctttttta tgtgccatat ctttaatttc ctcttattat attcagcaat atgatcatta
     5641 aacttaatca aagataagta atataacagc ttccattcat tatgtctata aaaaaaacaa
     5701 attttcctct gcaagattgc atctggataa tatgtatctt gtagattgac aacttcacag
     5761 taataaaggt gcctgatgtt ttggtcttag tatcctccca gcgggatttg taactgacat
     5821 tgggaataag agcgaacttt atcagcatta tcctttttac tgcactgatt gtaaacctgt
     5881 aaacgcaaga attagttttg tttacagttt ttcgactaat tattgtttgg cattgaattg
     5941 ccgaagctgt cggcactctt tagaagctgg ccatcgggct ggactttaag cgtatcggtt
     6001 gccagataga gattcaatgc cgcaacagca tattcgacta aagcatcggt tgcgttttct
     6061 tgcgcctcat agtaatcctc gtgagcatcc agcacatcac gcatattagc acgtccgact
     6121 ttttggagtc gcagggtgtt gtgcatccgt ttttgggcaa gttcctgcgc attcagagct
     6181 aagcggtact gctcatgtgc tgtccgaaga ttgcgaaatg ccgagcggac ttcctgaatc
     6241 acctgatcgg tgattccctg atgggtccgc tggttttgca ttaattcggc aagggtcaga
     6301 cggtagcggt tctgttccgc cttgcggtcc agaggcaggt ccatacgcag cgacaagttg
     6361 tacatatctt cggtgcggcg caagtcatcg ggtgaagcgc caaatgaccc aacagacgtt
     6421 gtgccgataa gtgccaaatc ggctccgagc atttctgctg caatttcgac atgacgctgg
     6481 gcgtccagca cacggtcggc cgcgttggcc aaatccagac gctggtttaa ggccgcctga
     6541 atcgccgctt cttcagagaa attcagctcc gcaaaatcaa atgccttcag ggcctccagt
     6601 tcctgtatgt ccagcacaag ctccgtatca gggcgaatag ccagcattgt tttcaatgta
     6661 tccagcattt gaccataatc gttgcggagg tcgatatagg tattcagcgc atcaagctga
     6721 tcctgatagg tctgctctac ttcaaacagc ggcactctgc ccacagcggc ctgtttttgg
     6781 gtttgccggt gaatttctgc aagcgtcagg taatagcggc gtgcattgtt catctggtcg
     6841 tagaactgaa gcgtttcata gtaggtttgg agaacagtaa caaggaatgt cttgcgatag
     6901 cgattgaaat agcgaatctg atagagcaag tcgcgttcgg cctgcgtcag tgtctccagc
     6961 acggccttac ggctggcccc cctcagcagc ggctgggtaa cggtggccga aaaaacgctg
     7021 gcaaagccgc ttttaatgct gccgtctcct cctgaaagaa ggtcaaagca cgtgaggctc
     7081 agattcgctc caacctgtgt ccctgcagcc agcagctgac gcagtcccag ccccagcccg
     7141 ccggcagtgc caacggtttc ctgcccatcc tgacggaggt agtcttcctg aatactgccg
     7201 cccggaatga tttcgtaaag atgctgaata tctgtaaccc cgctggccag ctgaaacaaa
     7261 ttatctcttt caagaaggta gtcacgattg tacgttacag ccatcgctgc tgcctgtgta
     7321 agagtcaaaa cactattctg cggcagcaca gcacgcaccg cctcgcgctg attgggatca
     7381 tccagccggt aataatcatt ctgctctaaa ccagccttct ttgtagcttt ttcaatagcc
     7441 ccgtaaattc gcttatccgc agcggaagcg gcctcttcag ggctgggtcc gcagccccac
     7501 accaacagaa ttcctgtcag caggaacact gcccagccgg ccttgtgccg tgtatttatg
     7561 gcagatagta ctggaattgc tttcacaggc cttttacgcc tacaatctca ttgggtacaa
     7621 gtttggttcc cggttgcaga gggataataa tcacctgtcc ccattccgga agcgtggggc
     7681 tttgccacaa ccgctccggc agcagttcca gcgaaggact gatatgcgta atgcgggatg
     7741 taaccgcctg ctggggtgtc cggcgggtaa cgatttgaac gggcatatct atatgcagca
     7801 ggggaatctt ttcctgcggt acccaagcgg cgacataatc cggtgcgggt ttgacgatag
     7861 tcattatcgg aatatcccgc atcactgcct gaccgggttt atagaacaga ctgctcacga
     7921 cgccgtcaaa cggtgccgtc aaaataaccg tatcagtcgg acgaataatt tcctcgagaa
     7981 ctttttcctg cacagtgatg gctttggcaa gcgggtcaag gattttggcg attgcagagg
     8041 gcaatgccgc cgactgcata aactcatcca tgcgaagctg tgcttgggca tagtccgcct
     8101 cggcctgtgc aaggagctgc tgataggact cgaccgtttt tcgcgtcacc tcgcattggc
     8161 ttttaatttt ctgaacttcg taggattcga tggcattatc cttgtaaagg tcttcggcaa
     8221 tctggacttc caattcaagg tctttgagta gagcacggtt gggttccagt tcggttttgg
     8281 cttccagaat gcttattcgc gctcgctcca catcgacggc cagccgtcgc tgtatgtcga
     8341 agatatcccg ctgccggtct atctgttcgg cctccaactg ggcttctgcg gcttttagtt
     8401 cggctttgag ccgctccaac tcggcctgtg cagttgccct gcgggcatcg atgagtgtcg
     8461 tatcatacat atcatttcca attgtactga tttggacaac cgccagcgtg tcaccctttt
     8521 ttacttcttc atacagccgt accggaaggg ccttgatata gccgttctcc gcaaccgtaa
     8581 tggcttgttc ctgaccgata acgaggccgt tgaaatccac acggcttccc tgctggataa
     8641 acaacactgt cactccgcca agtgccgcaa gccacacaaa caccggaatt gttttattca
     8701 ataatcgtcc tgatttaatt cgccgcatcg tgcattaaca ccccccatta aatttccagc
     8761 aattgttcct gaaccgtcaa gctgatgttc ttgacgccct cgatctgctg cagttcagcc
     8821 agcattgcct catttttgga aacgttttta atcataacct gataagcata ttcgacatcc
     8881 tgattgccga aatcgccatc ttgggcagaa attagagtaa aattgccgca gaattttttc
     8941 agtacccgca taatcggatg gtcagcgcca aaatggccgt taaagcggaa ccgcagaaag
     9001 gcattatgcg gtgtatgggt tccgaaatcc gatacatgca gatataaaat aatcagtgca
     9061 atgcaaaccg ttccgataac ggctatggaa taccgctgag agccgcaggc cattccaata
     9121 accagcgagc agaaaataaa ggcgatgtcc cgcgtatctt ttatcatatt gcggaaacga
     9181 ataatcgata atgcccccat cagccccacg gccaccacaa agctgcctgc tatggtggac
     9241 attaccattg ccacaatcac tgtaatgaga ataagcgact gcacgaatga acgcgaatag
     9301 gacagaccgc tgtgggtaaa atagtacagc cacgcgaggg cctgtcccag tacaaatgcc
     9361 agcagcagcg acagcagaac actctgcagc gaataattca ggctgtgtgc tgaggcgttt
     9421 tctataattt ccagtatatt attcatcata gcaccatatc gaacctgccg gcggcgagca
     9481 gtagcccagc tggcaggact gctgaaccga tgatacgtat tttgacatag acgattgctt
     9541 caagtcaaat attttaacca tttcactcag ccacagcggg tagcgcgatg taaatttgat
     9601 ttccagtacc acaaagtcca tagccaccga agtccaccct gtgccgttaa ccgaaacaac
     9661 gggctggcag gttgttttga agcacagctg ccggtcgaag gtaatccgaa cccgattatc
     9721 tgagtcgcct tcaaaggcct gccgcatata gcggaccagc acaatcggcc gtgcgctcag
     9781 agcggctgca tacatcataa attgattaaa tgcatccctg tctttggcgt aggttagagg
     9841 cgggatatac cggccggcca gaatatcgcc caattcggcc ttttttaccc ctacccggct
     9901 ttttacaatg acattgcagg cccgccgctt gatttccaga aatacaggag aaagcggatt
     9961 atcgtcatag ccgcggattc gcagcttaaa gcggtttttt ttgccctgca gggtctcatt
    10021 gcacaaggtt agcgtgggtg tatccaaata gagactggaa atcaagtatt cattgcccgg
    10081 tttgt
//
LOCUS       DAOTBO010000098         9987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5758_length_9987_cov_4.805679, whole genome shotgun sequence.
ACCESSION   DAOTBO010000098 DAOTBO010000000
VERSION     DAOTBO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_9987_cov_4.805679"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLE88_10295"
     CDS             complement(<1..328)
                     /locus_tag="PLE88_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOM76894.1"
                     /translation="MAKKIAINGFGRIGRLVFRAMVEQGLLGTKFDVVAVGDIVPADN
                     LAYLLKYDSVQGKFNGTVGSKKSAPDKADDDVLVVNGKEILVVSAKTPAELPWAKLGV
                     EIVIEAT"
     gene            complement(516..1922)
                     /gene="tilS"
                     /locus_tag="PLE88_10300"
     CDS             complement(516..1922)
                     /gene="tilS"
                     /locus_tag="PLE88_10300"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOM76895.1"
                     /translation="MLDAFEDNLCDWILAEGLIKPGSRLLLAVSGGADSVAMAAALCR
                     LRDEGRLTCDFIIGHVNHCLRGSEADDDENFVRRFSAALGLPAAVQRVDVRSYAAAQK
                     LSIETAARFLRLESLAAMAKEHRCDAAAAAHHQDDLAETVIYRLMRGTGFRGLCGIRP
                     ASEIFGTVFVRPLLNVRRSEIIRYCRRRHLDWREDATNRDTAFARNRIRLRLMPALED
                     RCIAERLSVLSAKCRRFVQRTEQLAQSVGQQGRWEKGGFVIEQSRLRSCPPWVMYELL
                     RRLPVQLGIGLRDYSREHFEAMRRLAGAQKSRIALPGPAEAFVEKGYFFYVKQIRNLV
                     LEPAVVQIGKTMEWGQWQISSRLFNRQEADCRHFFETKNALTEWFDADKVKGPITIRL
                     RQQGDRFWPIGADCEQKAARFLLKSGLDTLTRQNTVVIADAEKILWLAPVRASEQAKI
                     TLQTTKILEMTITPLRSI"
     gene            complement(2006..2827)
                     /locus_tag="PLE88_10305"
     CDS             complement(2006..2827)
                     /locus_tag="PLE88_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM76896.1"
                     /translation="MMRTSVIAAMVVPAMLLTGCENPRQRDAHLLGPQDIPALTEGQV
                     FLAPGTGEVDYVEKLAAARDAYRQALDALEGYYSSVGNATKLQWVKTEIKTFDQMVKY
                     QYLQPAEWTPENLTASDSIAEADALYRQATSLYNESGGMIIVANRAKLQQALGLFNQL
                     IQQYPTSDKIDDAAYRAGRIYEYLKNYELAAVYYQRTFQWNDATPYPARFRAAQVLDQ
                     KLKMRPQALALYQLAVARESRYVDNVEYAKARIAAMSAMSPEVETAPQAPAEPVQ"
     gene            complement(2989..3726)
                     /gene="ispD"
                     /locus_tag="PLE88_10310"
     CDS             complement(2989..3726)
                     /gene="ispD"
                     /locus_tag="PLE88_10310"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOM76897.1"
                     /translation="MKKASVIICAAGASSRFGGDRKKPFVDVAGRPAFLRSIDFFAGR
                     EDVVQILLAIPPDEQEMIEIKWGAALGFNAVKLCLGGAERFETVANALKKVSDEADLI
                     AVHDAVRCCLTSDWLDALFAKAAETGAAMLACPVTATLKKVQDGKIVDTVDRTGLYEA
                     QTPQVFDAKLLKDAYAKLDTLDKSKITDDAQLVEAAGCPVYIVETDHSNIKITRKSDI
                     PIAEAIIKSRPKPKPEGPIGPFIEAQW"
     gene            complement(3844..4725)
                     /locus_tag="PLE88_10315"
     CDS             complement(3844..4725)
                     /locus_tag="PLE88_10315"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOM76898.1"
                     /translation="MYCTKCGIQNSDGAVYCVNCGTCLMGTPGQGQQAAVQPRISRLA
                     IASLVMGLLCMTCVLCPILAFPAILCGIIALIKISGQRGVLKGTGMAITGIVIPLVMV
                     LFIPFFIGLLTPILLPALHKTQSIAQRTMCSSNLKALAAAMIFYAYSHEDVLPGENWC
                     DLLIEQANVSPQAFVCPASDAVIGESSYAMNNYIAGRKSHSLPPDMVMLFETSKGFES
                     GVQSESGKIRRQTGLLHYDQDGRLRKERFNQMGGPEDLLLRHDEGAASGCNVAFVDGH
                     VEFVRQDRISKLKWTAQ"
     gene            complement(4820..5581)
                     /locus_tag="PLE88_10320"
     CDS             complement(4820..5581)
                     /locus_tag="PLE88_10320"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOM76899.1"
                     /translation="MVYEDLKGKRVLVTGSSSGIGAATAVLFARQGSFVGVHCFRTPE
                     GGRKTLAEVKKYSDGCLLQADMRDEQQVRRLVADFVKTAGGIDILINNAGTLLGRVAI
                     EGMPLAHFEDEFATNVRSAFLATREALEHLKKSKGCIVNLGSVAGHHGGGGGSGIYAA
                     AKAAVHTMTIAMAKEFGPYGIRVNTVIPGLIETRFHEKFSTPQRKRETALQTPLGRNG
                     TAEDVAKAILFLASDAAGFITGEYLAVNGGLYMRA"
     gene            complement(5598..6089)
                     /locus_tag="PLE88_10325"
     CDS             complement(5598..6089)
                     /locus_tag="PLE88_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76900.1"
                     /translation="MMPLWTESQRWPWFLSLILLSGTVVLAAVVVLLILTTELPPSAK
                     TILGIAGLAAALLDILLASLRLVSEIYPDRIDIYFAPLHFPCRKIGWDEIEKIYARQY
                     SPVAEYGGWGIRCGKSGGAYNIKGNQGIQIELKNGRKFLVGTQQPDAFMQAVKSVYKQ
                     AAD"
     gene            complement(6086..7135)
                     /locus_tag="PLE88_10330"
     CDS             complement(6086..7135)
                     /locus_tag="PLE88_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOM76901.1"
                     /translation="MSDVLITVLILLVVGVGVLGAMILAAINAARRESSQSAAAVSAL
                     QQQIEAVRQKQDNLGQSLEKSLRSGQENISQFLTASQQTFGELKEQIGKIKGNSEHLL
                     QIGADIRTLQNLLNAPKLRGQIGEFSLASLLQKILPADSFTLQHAFSNGKIVDALVRL
                     PDFSVPIDAKFPLPAFEKMIAAPDEAEKTKLRRQFQADVVKHIDKIAASYILPDEGTL
                     DFALMYIPAENVYYETIIKYDNDRLDLLHYALDRKVVPVSPNLLYAYLMTIVMGLHGM
                     QIEKQAAAIRANLQRLAADFEAFGQTWDILGQHLRNAQNQYDRGQSRLNRFQLQLEQI
                     QQTGNAETKLPGDAE"
     gene            complement(7175..9703)
                     /locus_tag="PLE88_10335"
     CDS             complement(7175..9703)
                     /locus_tag="PLE88_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOM76902.1"
                     /translation="MSKAKAVLWMILVCVAAVCGAGGGGQESLPTAVISVDAGRSKPI
                     SPLLFGIFFEDINYAADGGLYAELVQNRSFEYSRGDNRSWNALTAWQLLQSSPEAASV
                     SVSTDAPLHPNNPHYAVLTLRQAGQGNGLRNSGFDGMVVKGGDKYDVSLFARVVSGSP
                     APITVRLESPDGAVLGQAQWTDLNAVWTKHTAVIEAAADASQAHLTVYAGGTGALALD
                     MISLFPQKTFKNRPNGLRPDLAQVIADLKPKFVRFPGGCVAHGDGLENMYRWKDTIGP
                     VEQRKSQRNIWRYHQSLGLGYFEYFQFCEDIGAEPLPVVPAGVCCQNSGNYLNLVPRG
                     QQGIPMDQMDAYVQEVLDLIEWANGPASSEWGVKRAAAGHPEPFNLKYLGVGNEDAIT
                     PVFKERFKMIHDAVKARYPDIVVIGTSGPFTDGRDFDEGWQFAREEKLAMVDEHGYKS
                     PGWFWDNLQRYDAYPRSGPKVYLGEYAAHDEGRLNTLRSALAEAAYMTSLERNGDVAA
                     MASYAPLLSKEGRTQWRPDLIYFDNVSITPSINYYVQQLFSVNQGDAYLPTTVNTAAV
                     RPTEKSAAGPSGILLGTWNTQAQFDNVKVVCGPTVVLEEAFDKPAGGWKTLSGRWTQA
                     DGVYRQTAPDMPALSMYACSGDNCTITLRAMKTGGAEGFLIGFGAKDAHNYYWWNLGG
                     WNNTSHAVEKITDGGKSTVGRSAAGRIEANRWYDIRIQMADNRIQCYLDGELIHDLTD
                     RGFVPTPDLAASAVREAKTGDVIIKIVSKADKAMPAEIDLSTLGRFRAQAVRTVLTGK
                     PEAVNRFGQTPEVLPQVWPMDVSEKFAYEIPPCSLTVIRVKMPR"
     gene            complement(9594..9905)
                     /locus_tag="PLE88_10340"
     CDS             complement(9594..9905)
                     /locus_tag="PLE88_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76903.1"
                     /translation="MMPQPLKQRFKRPFADRLQNWIARVNPLLIIVRRAVWRCQVRSH
                     RGGVFPYLATSRQRFSRKHKGQDYEQSKSGFMDDFGVCGGGLRCRRRWSGIPADSSYL
                     R"
BASE COUNT         2238 a         2943 c         2537 g         2269 t
ORIGIN      
        1 cggtggcttc aataacaatc tcgacaccca gtttggccca cggcagctcg gcaggggtct
       61 tggcactgac gaccagaatt tccttgccgt taacgaccag aacatcatca tctgccttat
      121 ccggtgcaga cttcttggag ccgactgtgc cgttgaattt gccctgcacg gaatcatact
      181 tgagcagata ggcaaggttg tcagccggga cgatatcgcc gaccgctact acatcaaact
      241 ttgtccccag cagtccctgc tcgaccatag cacggaatac aagacggccg attcggccga
      301 aaccgttaat cgcaatcttt tttgccattt acaatctcct ttaatttaaa aattggttaa
      361 taaaggcatc catcgataca ccatctccgc ttatccggag acaaacagag ccactaatgt
      421 agaacccaac aggaactttg tcaagcaata tatactaaat tattgcccgc aacttaacaa
      481 ttttgtactg gactataacg atacatatta accttttaga tgcttcttaa aggagttatc
      541 gtcatttcca atattttcgt tgtctgcaaa gtgatttttg cctgttcgga tgccctgact
      601 ggggctaacc aaagtatctt ttctgcatct gcaataacga cagtattttg ccgcgttaac
      661 gtgtccagtc cggatttcag gagaaaacgg gcggctttct gttcgcagtc ggcaccaatt
      721 ggccaaaacc ggtcgccctg ctgtcttaag cgtattgtta tcgggccttt gactttatcc
      781 gcatcgaacc actcggtcaa ggcgttcttg gtttcaaaaa aatgccggca gtctgcctcc
      841 tgccgattga aaagacggga cgaaatctgc cattgtcccc attccatggt ctttccaatc
      901 tggacaacgg ccggctccag aaccagattg cggatttgtt tgacatagaa gaaatatccc
      961 ttttcaacaa atgcttcggc cgggccgggc agggcgattc tggacttttg ggcacctgca
     1021 agccgacgca tcgcctcaaa atgctccctc gagtaatccc gcaatccgat gcccagctga
     1081 accggcagcc gccggagaag ttcatacatc acccaaggcg ggcaggatcg cagccggctc
     1141 tgctcgatga caaatccccc cttttcccat cgtccctgtt ggccaacgga ttgagccagc
     1201 tgttctgttc gctgcacaaa ccgacggcac ttggcagata agacagacag ccgttctgct
     1261 atgcagcggt cttccaatgc cggcatcagg cgaaggcgaa ttctgtttcg cgcaaacgcc
     1321 gtatcgcggt ttgtggcatc ttcccgccag tccagatgac ggcgtcggca ataccggata
     1381 atctcgctcc ggcggacatt cagcagcgga cggacaaaca ctgtcccgaa aatctctgaa
     1441 gccggccgaa tgccgcacag acctcgaaag ccggttcccc gcatcaggcg gtaaatcacc
     1501 gtctcagcca aatcgtcctg atggtgcgcc gctgccgccg catcgcagcg atgttctttt
     1561 gccattgccg ccagcgattc cagccgcagg aagcgtgcag cggtttcaat ggaaagcttc
     1621 tgggccgcgg catacgaccg cacatcgacc ctctgtacag ccgccggcag ccccagagcg
     1681 gcgctgaagc ggcggacaaa gttctcatca tcatcggctt cgctgccgcg caggcaatga
     1741 ttgacgtggc cgataataaa atcgcaagtc aggcggcctt catcccgaag ccggcacagc
     1801 gcagcagcca ttgcaaccga atccgctccg ccggagactg ccagcagcag ccggctgccc
     1861 ggttttatca ggccttctgc aaggatccaa tcgcacaggt tatcttcaaa cgcatcgagc
     1921 atcggcaact aactgttttc acggatgcaa taaaaaaggc ctgttacatc aacaggccct
     1981 ttgaatgtct gcggacagca tcttcttatt gaaccggctc agccggagct tggggagctg
     2041 tttccacttc cggagacata gccgacatcg ccgcaatgcg tgccttggca tattcgacat
     2101 tgtccacata ccgagactcc cgggcaacag ccaattgata cagcgccaag gcctgcggac
     2161 gcatcttcag cttctgatcc agtacctgtg cggcgcggaa gcgtgccgga tagggcgttg
     2221 catcattcca ctggaatgtc cgctgataat acaccgccgc cagttcgtag tttttaagat
     2281 attcataaat gcgcccggcc cgataggcgg catcatcgat cttatcgctg gtcggatact
     2341 gctgaatcag ctggttgaac aagcccaatg cctgctgcag cttggcgcga ttggctacaa
     2401 taatcatccc gccggactca ttgtaaagcg aggtcgcctg ccggtagagg gcatccgctt
     2461 cagcaatcga atcggaagct gtcaggtttt cgggcgtcca ctcggccggc tggaggtatt
     2521 ggtattttac catctggtca aacgtcttga tttctgtctt gacccattgc agcttggtgg
     2581 cattgccgac agagctgtaa tagccctcca aagcgtccag cgcctgccgg tatgcatcac
     2641 gggcggcggc cagcttttca acataatcga cttcccctgt gccgggtgct aagaacacct
     2701 gaccctctgt gagggcggga atatcctgcg gaccgagaag atgagcatcg cgctggcggg
     2761 gattttcgca ccctgtcagc agcattgcag gcacaaccat agcagcgata acacttgttc
     2821 gcatcatagc ttaactcctt tcgctttcag cttgattcct gttctttggg tattaacgat
     2881 accagctttt ggccggctgt caagcataat atacagcggg atttttgacc cggaatataa
     2941 aaaatcggcg atcgcagcct ttcagaattg acctttagac cggcgatttt accactgggc
     3001 ctcaataaaa ggccctatgg ggccttcggg ttttggcttc ggccgggatt tgatgatggc
     3061 ctcggcaatg ggaatatcgc tctttcgcgt gattttgata ttggaatgat cggtttcgac
     3121 aatgtacaca gggcagccgg cggcttcgac taattgggca tcgtcggtga ttttagattt
     3181 atccagcgtg tccagtttag cgtaggcgtc cttgagcagc ttggcgtcaa aaacctgcgg
     3241 cgtctgggct tcatacaggc cggtccgatc gactgtgtcc acaatcttgc cgtcctgcac
     3301 ctttttaaga gtagccgtaa ccgggcatgc cagcatcgcc gctcctgttt ctgcggcctt
     3361 ggcgaacagg gcgtccagcc agtcgctggt caggcagcag cgaacagcat cgtgcacggc
     3421 aatcaggtcg gcctcgtcgc tgaccttttt gagggcgttg gcgacggtct cgaaccgctc
     3481 ggccccgccg agacaaagct tgacggcatt aaaccccagt gccgcccccc acttgatttc
     3541 tatcatttcc tgctcatccg gcggaatggc cagcagaatc tgcacaacat cctcgcggcc
     3601 ggcaaaaaaa tcgatgctgc gcagaaacgc aggccggccg gccacatcca caaacggctt
     3661 tttgcggtcg ccgccgaaac ggctgctcgc accggcggca caaatgataa cagaagcttt
     3721 tttcatggca ttctttttta gtcgggatca acagaattta caagaacttt ttcacatacc
     3781 ggcaagggca tatccctgcc ccgacggcat cgatacaaaa acgccggcac cgctgcgctg
     3841 gtgttattgt gctgtccact tcagcttcga aatgcggtct tgacggacaa actcgacatg
     3901 gccgtcaaca aaggcgacat tgcagccgga ggcagctccc tcatcatgcc tcagcagcag
     3961 gtcctccggc cctcccatct ggttgaaccg ttctttgcgg agacggccat cttggtcata
     4021 gtgaagcaat ccagtttgcc gccggatttt tccagattca ctttgaaccc cgctttcgaa
     4081 gcccttgctg gtctcaaaaa gcatcaccat atcgggcggc agactatgcg attttctgcc
     4141 ggcgatatag ttgttcatgg catacgaact ttcccctata acagcatcgg aagccggaca
     4201 cacaaatgcc tgcggcgaca cattcgcctg ctcaatcagc aaatcgcacc aattttcccc
     4261 cggcagcaca tcttcgtgac tatatgcata aaaaatcata gccgcggcta atgctttcaa
     4321 attggagctg cacattgtcc gctgggcaat actctgtgtc ttatgcaaag ccggcagcaa
     4381 aatcggcgtc agcaacccaa tgaaaaaagg gataaaaaga accatcacca acggaataac
     4441 gatgcccgtt atcgccatgc cggttccttt gaggacgccg cgctggccgc tgattttgat
     4501 taaggcgata atgccgcaga gaatcgccgg aaacgccaaa atcggacata ggacacaggt
     4561 catgcacagc agacccatca ccaaagatgc tatcgccagc cggctgattc ggggctgcac
     4621 ggccgcctgc tgcccttggc ccggtgtgcc catcaggcaa gtcccgcaat tgacgcaata
     4681 taccgccccg tcggaattct gaatgccgca tttcgtacaa tacaaaccag gtctcctttc
     4741 agcacaatat cctcacttct cagtgctcac ttataaacac tctcacccga aagccgatgc
     4801 tgaaaattcc gtacgaattt tatgcccgca tataaagtcc gccgttgacg gccagatatt
     4861 cgccggtgat aaagccggcc gcgtcgcttg ccagaaacaa gatggccttg gccacatctt
     4921 cagcggttcc gttgcgtccc agcggggtct gcagggcagt ttctcgtttg cgctgcgggg
     4981 tggaaaattt ttcatggaag cgggtttcaa tcagcccggg aataacagta ttgacccgaa
     5041 tgccgtaggg tccgaattcc ttggccattg caatggtcat tgtatggacg gcggctttgg
     5101 cggcagcata aatgcctgaa ccgccaccgc cgccgtggtg tccggccact gagccgagat
     5161 tgacgatgca tcctttggat ttcttcaaat gctccagcgc ctcgcgggtc gccagaaacg
     5221 cactgcgaac attggtggca aactcatcct cgaaatgcgc cagcggcatt ccttctatcg
     5281 ccacccgtcc caagagtgtg ccggcattgt taatcaggat gtctatcccg ccggcggtct
     5341 tgacaaagtc cgccaccagc cgccggacct gctgttcatc gcgcatatct gcctgcagca
     5401 gacagccgtc ggaatacttt tttacctccg ccagcgtttt tcggccgcct tcgggcgtgc
     5461 ggaagcaatg caccccgaca aaacttccct gccgggcaaa cagaaccgct gtcgctgctc
     5521 cgattccgct gctggagccg gttaccagaa cccttttgcc ttttaaatct tcatatacca
     5581 cagtgcaatt ccttcagtca gtccgcggct tgtttataga ccgattttac tgcctgcata
     5641 aaggcatcgg gctgctgcgt gccgaccaga aacttccggc cgtttttcag ctcaatctga
     5701 atgccctgat tgcctttgat gttataggcg ccgcctgatt taccgcaccg gatgccccag
     5761 ccgccgtatt ccgcaaccgg cgaatactga cgggcatata ttttttcaat ctcatcccag
     5821 ccaatctttc ggcaggggaa atgcagcggg gcaaaataga tgtctatccg gtccggataa
     5881 atctcgctga ccagccgaag cgaagccagc agaatatcca gcagcgcagc cgccagccct
     5941 gcaatcccca gaatggtctt ggccgaaggc ggcaattcgg ttgtaagtat cagaagcaca
     6001 actacagcgg ctaaaacaac cgttcccgac aatagaatca acgataaaaa ccacggccat
     6061 cgctggcttt ctgtccatag cggcatcatt ctgcatctcc gggcaatttg gtttcggcgt
     6121 ttccggtctg ctgtatctgt tccagctgca attggaagcg attgagcctg ctttgccctc
     6181 tatcatactg attttgggca ttgcgaagat gctggccgag aatgtcccat gtctggccga
     6241 atgcctcaaa gtccgccgcc agccgctgca gattggcacg aatggcagcc gcctgctttt
     6301 caatctgcat cccatgcagc cccatcacaa tggtcatcag gtaagcatac agcagattcg
     6361 gcgaaacagg tacaactttc cggtcaaggg cataatggag cagatccaga cggtcattgt
     6421 cgtatttgat aatggtttcg taataaacat tttcagccgg aatatacatc agggcaaaat
     6481 cgagcgtgcc ttcgtccggc aggatataac tggcggcaat cttgtcgatg tgcttgacaa
     6541 catctgcctg aaactggcgg cgcagctttg tcttttcggc ctcatccggt gcggcaatca
     6601 tcttctcgaa ggccggcaga ggaaatttgg catcaatcgg cacggaaaaa tcaggcagcc
     6661 gaaccagtgc atccacaatt ttgccgttcg aaaaggcatg ctgcagggta aaactgtccg
     6721 caggaagaat tttctgcagc agcgacgcca gcgaaaactc ccctatctga ccgcgaagct
     6781 tgggggcgtt gaggagattc tgcaaggtcc gtatatcggc gccgatttga agcaggtgtt
     6841 cgctgttgcc tttgatcttg ccgatctgtt ctttcagttc gccgaaggtc tgctggctgg
     6901 cagtcagaaa ttggctgatg ttttcctgac ccgaccgaag actcttttca agggattgcc
     6961 ccagattgtc ctgtttctgc cgaaccgcct caatctgctg ctgaagagcc gataccgccg
     7021 ccgccgactg ggatgattcc ctccgcgcag cattgatggc cgccagaatc atcgccccca
     7081 gaacgccgac accgacaaca agaagtatta aaaccgttat caggacatcc gacatcgctt
     7141 aaatatcctc atgcaggcac tctgcctact gccttcagcg gggcatttta acccgaataa
     7201 cagtcaaaga gcaaggcgga atttcatacg caaacttctc ggatacgtcc atcggccaaa
     7261 cctgcggcag cacttcgggg gtctgcccaa agcggttaac agcctccggc ttgcccgtca
     7321 ggacagtccg aacggcttga gcccggaagc ggcccagcgt gctcaaatcg atttcagccg
     7381 gcattgcctt gtctgccttg ctgacaattt taataatgac atcgccggtt ttggcttccc
     7441 gtaccgccga agcggccaaa tccggcgttg ggacaaaccc cctgtcagtc aagtcgtgta
     7501 tcagctcgcc gtccagatag cactggatgc ggttatcggc catctggatt cggatatcgt
     7561 accagcggtt ggcttcaatg cggccggccg ccgagcggcc gacggtgctt ttgccgccat
     7621 ctgtaatctt ctcgaccgca tggcttgtat tgttccagcc gcccagattc caccagtaat
     7681 aattgtgggc atcttttgcc ccaaagccga ttaaaaatcc ttccgccccg ccggttttca
     7741 tcgcccgcag cgtaatggta cagttatccc cgctgcatgc atacatcgac aaggcgggca
     7801 tatcgggggc tgtctgacgg taaacgccgt cggcctgcgt ccagcggccg ctgagcgtct
     7861 tccagccgcc ggcgggcttg tcgaatgcct cctccaaaac aacagttggg ccgcagacaa
     7921 ccttgacatt gtcaaactgc gcctgtgtat tccacgttcc cagcaatatg ccgctggggc
     7981 cggcggcaga cttttccgtt ggccggacag ccgccgtgtt gaccgttgtc ggcagatacg
     8041 catcgccctg attgacgctg aacagttgct ggacatagta gttaatcgaa ggcgtaatcg
     8101 agacattgtc gaaatagatt aaatccggcc gccactgcgt ccgtccttct ttcgacagca
     8161 gcggggcata cgatgccatc gccgccacat cgccgtttcg ctccagactg gtcatataag
     8221 ccgcctcggc cagcgccgag cggagcgtat taagacggcc ttcatcgtgg gcggcatatt
     8281 cgcccagata cactttggga ccgctgcgcg ggtaggcgtc ataacgctgc agattgtccc
     8341 agaaccagcc cggcgacttg tagccgtgct catcgaccat tgccagtttt tcttctctgg
     8401 caaactgcca gccttcgtca aaatcccggc cgtccgtaaa cggtccgctg gttccaatga
     8461 ccacaatatc aggataccgc gctttgacgg cgtcgtgaat catcttgaag cgttctttga
     8521 agactggcgt aatggcatcc tcgttgccga ctcccagata tttcaggttg aacggttcgg
     8581 gatgtccggc tgcggcccgc ttgacgcccc attcggagga ggccggcccg ttagcccatt
     8641 cgataagatc caaaacctcc tgcacatagg catccatctg gtccattgga atcccctgct
     8701 ggccgcgggg aacaaggttc aggtaattgc cggagttctg acagcagacg ccggccggca
     8761 ccaccggcag cggctcggcg ccgatatcct cgcagaactg aaaatattcg aaatagccca
     8821 agcccagcga ttggtgatag cgccagatgt tgcgctggga cttccgctgc tcgaccggcc
     8881 cgatggtatc tttccagcgg tacatatttt ccaaaccgtc gccgtgggct acacagccgc
     8941 ccggaaagcg gacaaacttc ggcttcaaat cggcaatcac ctgcgccaag tccggccgga
     9001 gcccgttggg gcgattctta aaagtcttct gcggaaacag cgaaatcata tccaaagcca
     9061 acgcgccggt gccgccggca tataccgtca gatgtgcttg ggatgcatcg gcggcagcct
     9121 caatgacagc cgtatgcttg gtccatacag cgttcaaatc cgtccactgg gcctgcccta
     9181 aaaccgctcc atccgggctt tccagccgga ccgtaatcgg cgccggactg ccgctgacaa
     9241 cacgggcaaa cagcgagaca tcgtatttat cgccgccctt gacaaccatt ccgtcgaagc
     9301 cgctgttgcg aaggccattg ccttgtccgg cctgacgaag cgtcaaaacg gcataatgcg
     9361 gattgttggg gtgcaggggt gcatctgtgc tgacggacac agaggccgcc tcgggactgc
     9421 tttgcagaag ctgccaagcc gtcagggcat tccacgaacg gttgtcgccg cggctgtatt
     9481 caaacgagcg gttctgcaca agctctgcat acagaccgcc gtcggcagca tagttaatgt
     9541 cttcaaaaaa gatgccgaac agcagcgggc tgatgggctt cgaccggccg gcatcaacgg
     9601 agataactgc tgtcggcagg gattcctgac caccgccgcc ggcaccgcaa accgccgcca
     9661 cacacaccaa aatcatccat aaaaccgctt ttgctttgct cataatcctg tcctttatgc
     9721 tttcggctga accgctgccg gcttgttgcc aaatacggga aaacgccgcc gcgatgtgaa
     9781 cggacttgac accgccagac tgcacgtctg actattataa gcagcggatt gacgcgtgca
     9841 atccaatttt ggagcctgtc cgcaaacggc cgcttgaatc tctgcttaag cggctgcggc
     9901 atcatcaaga acagcagttc ctgccgactg tgcggctgct gctttcgtta ttccaacagt
     9961 gcggctatgt cctctgcgct gagcggc
//
LOCUS       DAOTBO010000099         9782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5910_length_9782_cov_4.629793, whole genome shotgun sequence.
ACCESSION   DAOTBO010000099 DAOTBO010000000
VERSION     DAOTBO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5910_length_9782_cov_4.629793"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1135
                     /locus_tag="PLE88_10345"
     CDS             98..1135
                     /locus_tag="PLE88_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOM76904.1"
                     /translation="MRIGIIGFGFMGRMHLGCWSQLPQVEVAAICDANPDIAQTLDAA
                     LGNIPGLPGRIDPANLRFYPNIDRMLRAEQLDAVSITLPTYLHAELSCSLLKAGLHVL
                     CEKPMALNTGQCVQMIETARQTGKKLMIAHCIRFWPEYAHTRGIIQSGQYGKVYTARF
                     QRFSRPPAWSSGNWLMDSSKSGGMPLDLHIHDADYIQAVFGMPRAVCSCSVPRDGRID
                     HIETHYLYDSDMLVTAEASWLASDSFGFRMSYEVQAEHATIVYDSSQSPSYRIFPDDG
                     PPLTPSLSSKDGYFHEIQYFVRFIQGCIEKESMTLQEAMNSVGLIEAELESCKSGQRV
                     ELKNSAKRSPQ"
     gene            1132..1950
                     /locus_tag="PLE88_10350"
     CDS             1132..1950
                     /locus_tag="PLE88_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOM76905.1"
                     /translation="MKRCAGWPISVCTWSMSNDFCRLDVLMKETGIDHVHLDLRPVCT
                     NPSKNYIDEVCRRGWAFSAAMIGFEQEDYSTLETIRATGGIVPDACWPQNKAYFLKAI
                     ETAARLGIGFLSAHIGFIDHASIGSYKTLLDRMTLLADAAAQKNVMLLMETGQETAEE
                     LRQFLNDLNHPAVGINIDPANMILYGKGDPVEAVGILAHWIRHVHIKDAIASQIPGQW
                     GTEVVWGTGQVGAEMFLSALQEIGYRGAVAVEREAGQTRHDDIRVAIERLIHFR"
     gene            1988..3238
                     /locus_tag="PLE88_10355"
     CDS             1988..3238
                     /locus_tag="PLE88_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOM76906.1"
                     /translation="MTKTVRYQLSGMMFLEFFIWGAWFVTLWRYLNRLNFDGTWIGAA
                     YSTTGWAAVISPFFVGMIADRFFPAQKVLACLHLAGAVLMGWASAVTEPFLFFWILLA
                     YTVCYMPTLALSNSIAFSQMNDPETEFPPIRVLGTIGWIAAGLLVGFMPKSVFGLATI
                     EDTAIPMRIAAGTSLLMAFYCLFLPHTPPKPTGQKVRAADILGFKAIGLLKDPSFAVF
                     ITCSLLICIPLSFYYQSANGFLGQIGMDNPAAKMTLGQASEFVFMLLMPLFLSRLGIK
                     RLMLVGMAAWVLRYVFFAFGDTSSRMLSSFLYLGILLHGVCYDFFFVTGQIYVDKKAP
                     AEIRASAQGFLAFVTLGVGMIIGNLINGLVTRSCAVSTVIDGVQTTSYNWRTIWLIPA
                     VMALAVFVLFALTFSERTSRAASN"
     gene            3264..3530
                     /locus_tag="PLE88_10360"
     CDS             3264..3530
                     /locus_tag="PLE88_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76907.1"
                     /translation="MPACLQIIGKLLGDQCRPIVTQQRGSVFHRHLLKSGLPAASADA
                     AVMQPKTLAGHACAAGGCAENGVGMQPAAVCSRPRKLPQAGENG"
     gene            3587..7291
                     /locus_tag="PLE88_10365"
     CDS             3587..7291
                     /locus_tag="PLE88_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOM76908.1"
                     /translation="MNGKAFLGAAAVLAAVISLPSSAAVLSLHTNQPSVGACDIASLV
                     GAAADRDNVGTATADGYGNDQTTYIAFDRPAQGQTFVTPSGFPKYRLRAVTVRHAGYT
                     ANTSITWYRMDAGSRLTIRITNPALAGTAGFVLASEGYVLTGTEPNIFSPNTITNTAN
                     GTGTWVTLTLDAPAELAPNTLYGFDIGSDSAFFETLGIKESVSNPYPAGWAYISGASG
                     AGSNACTAAAGDRVFIVHLDAVIPDGGDLTGNGRVDLEDVALLSGGWQHLYGLTALAQ
                     IAAHWLFTEPPVFKSDPILAADAQAGAAYSASLTDAVIYYNPLRLVFAKISGPAWLTV
                     SPEGRLSGCPSAADVGLASAVVQADDGMHPPVQATVQIAVVQQPLQAVPFTQVTFADA
                     FWQPRLQTNRQVTIPYIFSKLQETGRIDNFAIAGKLMTGTPRYDFPFDDTDIYKTLEG
                     ASYSLMVTPDPALEAYLDELIVKIAAAQEHNGYLPGYLYTIRTNGVDIWCGPAPWTNL
                     SMSHELYNAGHLFEAAIAHYQATGKTALLNVAKQFADLLVDTFHDGGVEIPPGHEIVE
                     SALARLSDTAADKRYLDLARYYLDIRGTVTDDYSPWGQYYQDHLPVLQQTEAVGHVVR
                     AVYLYMGMADVAARTADGTYRKALMQALDAIWHSVNDTKAYITGGLGASHGGESFGAP
                     YYLPNDGYCETCAQIANVMWNHRMFLYHRDGKYIDVLERTLYNALISGVSLKGDTFFY
                     PNPLVSSGGYTRSAWFSCNCCIGNIARTIPSIPGYVYARGDDAIYINLYAAGTGQVQL
                     DQTAVRLTQTGNYPWDGTMTITVQPDVPADFTIYVRIPGWAQNQPVPGDLYAYRDAQP
                     LTPSLDVNGTPVALAVQKGYVAVSRTWQAGDQIHLSLPMAVRQVQAHPNIAADAGRIA
                     LERGPIVYCAEWPDFEGRTITHLYIPDGADFSVEYRDNMLGIPELPNAGAVIKGTVKG
                     LYEAAGGAVEQRDVPFTAIPYYAWAHRGPGQMAVWLPAGPDKAVPIPQPTPAELVGHW
                     KLDETAGTTAADSSGKQMNGTLLNGLSFSSNAVAGPVGNALSFDGSNDYIDLPDGFSD
                     FAKGCTITLWARPTAVKNWARFVDLGNGSAADNIWFGRQGTTGNLAFECWAGSSSSGL
                     VTAAGAIALNEWQMFAVTADSFGHVKLFKNGQLIQTGSSAPSSVTRTRNFIGRSNWSA
                     DAYYQGALDDIRIYNYNLSDDAVMQLYTGL"
     gene            7319..>9782
                     /locus_tag="PLE88_10370"
     CDS             7319..>9782
                     /locus_tag="PLE88_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOM76909.1"
                     /translation="MTAFKERCWMRLLCGMLAVSWAAADITYTFHLDGVDAGIANTIK
                     ASVAEAVGLYNQHGSFNKHLYIYYNAGVPTAQANYDGVITFGGSRNTRVALHEMAHTL
                     GTGTVSAWSANLSGGVWQGYYAAKLALAQGSPYADGLHGDGAHFWPWGLNYDSEDGFM
                     ERIYHIRTVAALRCDMGILAFALEPRHQTADLGGTAVFSAAAPAAASWRWYKDGVALT
                     DGGRISGAAGPELRIAQAELTDEGSYSCAATGAGETLFSRPARLTIRKLAAYYKFNGN
                     ANDSAASNHAAAFGSPLYSAGMAGQAIDLDGTDDYVMLPAGIADTDDITVGGWVNWDG
                     GGNWQRIFDFGTGTAQYLFLTPSSDSNRLRFAIKDSGGEQIVETARLAVGQWVHLAVV
                     LQDTTAVLYVNGRPAAHSGTVTIDPRNFAPNRNYIGKSQFSADPLFNGRIDDFRIYTC
                     ALDGADIWNWWGQSADSAPRFAADPLILPDGAQGTAYTGQTLAAWAEDADGDTLTFSK
                     ISGPAWLSVSAGGLLSGTPGSTDAGKNTFIVRAADPFGACTDTQLIIYVHGLAAAHYP
                     FEGNALDQSGGGWNGTASGGPAYTSGFFGQAIDLDGIDDYVVLPAGIIRTADFTVAAW
                     VYWDGGGSWQRIFDFGNNTTRYMFLTPSSPSGTLRFAVTAAGNGREERVETAALTPGA
                     WTHVAVSRSGQTLAMYVNGTRRAVNPVSTVSPADFAPAYNYIGKSQWPDPLFNGRIDD
                     FRIYNYALSPAEIAVLSSTSIFTDEQFDNDQAAELAPYAGRPLTAFTDYAGGSGALVF
                     AKVSGPAWLFVSPDGTLSGIPAD"
BASE COUNT         1938 a         2836 c         2894 g         2114 t
ORIGIN      
        1 ctatgctaac ggaagggacg ctttatttgt cgtggagaaa ttatagttga cgctggatac
       61 tggatgcata agatacggca tacggggaga tttgtgcatg cgaatcggga ttattggttt
      121 tggatttatg gggcggatgc acttggggtg ctggtcgcag cttccgcagg tcgaggttgc
      181 ggccatttgc gatgccaatc cggatattgc tcaaactctg gatgcagcgc tggggaatat
      241 tccgggactg ccgggcagaa tcgacccggc aaacctccgc ttttacccaa acattgaccg
      301 gatgctccga gctgagcagc tggacgccgt ttccatcacg ctgccgacct atctgcacgc
      361 agagctttcc tgctctctat tgaaagcagg acttcatgtc ttgtgcgaaa agcccatggc
      421 cttgaataca ggccaatgcg tacaaatgat tgagacggcc cgtcagacgg gcaaaaaact
      481 gatgattgcc cactgtatcc gcttctggcc cgaatatgct cacacacgcg gcatcataca
      541 gtccggtcaa tatggcaaag tctataccgc ccgattccag cggttttcaa gacctccggc
      601 atggagcagc gggaactggt tgatggattc atccaaaagc ggcgggatgc cgctggatct
      661 gcatatccac gatgccgatt acattcaggc cgtctttgga atgccccgtg ccgtctgcag
      721 ctgttccgtg ccgcgggacg gccgcataga ccacatcgaa acgcactatc tctacgattc
      781 ggatatgctt gtaactgccg aggccagctg gctggcttcg gattcgttcg gattccggat
      841 gagctatgaa gtacaggccg aacatgccac gattgtctat gattcgtccc aatccccttc
      901 gtatcgcatt tttccggatg acggcccgcc cttaacgccg tcgctttcct ctaaggacgg
      961 ttattttcat gagattcaat attttgtcag gttcattcag ggatgcatcg agaaggaaag
     1021 catgacgctg caggaagcca tgaattccgt cgggcttatt gaagcagaac tggaatcgtg
     1081 caaatccgga cagcgtgtcg aattaaagaa ctcagcgaaa aggagccccc aatgaagcgc
     1141 tgcgccggct ggcccatcag tgtctgcaca tggtcgatga gcaatgattt ttgccgcctt
     1201 gatgtgttga tgaaggaaac cggcatcgac catgttcatc tggacctgcg gccggtttgt
     1261 accaacccgt cgaagaatta cattgacgag gtttgccgcc gcggctgggc gtttagcgct
     1321 gccatgatcg gatttgagca ggaagattac tcgacgcttg agacgattcg tgccaccggc
     1381 ggcatcgttc cggatgcctg ttggccgcag aataaggcct attttctgaa agccatcgaa
     1441 acagccgccc gactggggat cggcttttta tccgcccaca tcggatttat tgaccatgcc
     1501 agcatcggca gctataagac attacttgac cgcatgacac tgctggctga tgcggccgcc
     1561 caaaaaaatg tgatgcttct gatggaaacc ggccaggaga cggccgagga actgcgtcag
     1621 ttcctcaatg atctgaatca cccggctgtt ggcatcaaca ttgacccggc caatatgata
     1681 ctttacggca aaggcgatcc cgtcgaggcg gttggcatat tggcacattg gattcggcat
     1741 gtccatatta aagacgccat tgcctcgcag atacctggcc aatggggaac tgaagtggtc
     1801 tggggcacag gccaggtcgg ggctgaaatg tttctgtccg ctctccagga gataggctac
     1861 agaggagccg ttgctgttga acgtgaagcc ggacaaacac gccacgatga tattcgtgtc
     1921 gctatcgagc gtttaatcca ttttcgataa cctgcatcct tcactttaac aacaaaagga
     1981 ataaaaaatg acaaaaacgg tgcggtacca gctttcggga atgatgtttc tggagttttt
     2041 tatctggggg gcttggtttg tcaccctgtg gcgctatctg aaccggctga atttcgacgg
     2101 cacctggatc ggcgctgcct acagcaccac cggctgggcc gccgtcatca gccccttttt
     2161 cgtgggcatg attgccgacc gttttttccc cgcccagaag gtccttgctt gtctgcactt
     2221 ggcaggcgct gttctgatgg ggtgggcctc cgccgttacg gagccgtttc tgttcttttg
     2281 gatccttctg gcctatacgg tgtgttatat gcctacgctg gccctgagca attccatagc
     2341 gttttcacaa atgaacgatc cggagaccga atttccgccg attcgcgttt tgggcaccat
     2401 cgggtggatt gctgccggcc tcctggtcgg gtttatgccc aaaagcgttt tcggccttgc
     2461 tacgattgaa gacaccgcta tccctatgcg gattgccgcc ggcacgtcat tgctgatggc
     2521 gttttattgc cttttcttgc cgcatacccc gcccaaacct accggtcaaa aggttcgcgc
     2581 tgcggacatt ctcggtttta aggccatcgg cctgctcaaa gacccctcgt ttgccgtctt
     2641 tattacctgc tcgctgctga tttgtatccc tctttctttt tattaccagt cagccaacgg
     2701 tttcctgggc caaatcggca tggacaatcc cgccgccaag atgacgctcg gccaagcctc
     2761 ggaattcgtt tttatgctgc tgatgcccct atttctctcg cggctgggca tcaaacggtt
     2821 aatgctggtc gggatggcgg cttgggtgct gcggtacgtg ttttttgcat tcggtgatac
     2881 ctcgtcgcgg atgctgagtt cattcctcta tcttggcatt cttctgcacg gcgtctgtta
     2941 tgatttcttt tttgtgaccg gccagattta tgtagacaaa aaagccccgg ccgagatacg
     3001 tgccagcgcc cagggttttc tggcctttgt gaccttgggt gtcggaatga tcatcggcaa
     3061 tctcatcaat gggcttgtca cacggtcttg cgccgtctcg actgtaatcg acggcgttca
     3121 aacgaccagc tataactggc ggacaatctg gttaatccct gcggtgatgg cactggcggt
     3181 gtttgtcctg tttgccctga ccttttctga acgaacctct cgtgcagcgt ccaattgatc
     3241 tgtgcaaagc cggaatatgg ccgatgcccg catgcctcca gataatcggc aaactccttg
     3301 gagaccagtg ccgacccatt gtcacccagc agcgcggttc tgttttccac cggcatctcc
     3361 tgaagtccgg tcttccggcg gcgtcagcag acgcagccgt aatgcagccg aaaacactgg
     3421 caggacacgc ctgcgcagcg ggcggctgtg ccgaaaacgg cgttggaatg cagccggcag
     3481 cggtttgcag ccgcccgagg aaattgcccc aagcggggga aaatggttga aaaaaaccgg
     3541 ccggcagggt ataattattg taaaaattaa acagggggtt ttacggatga acggaaaagc
     3601 atttctgggg gctgcagcag ttctggcagc ggtgatttca cttccctcgt cggcggcggt
     3661 gctgtcgctg catacgaacc agccgtcagt cggggcctgc gatatcgcat cccttgtcgg
     3721 cgcagcggca gaccgcgaca atgtgggaac ggcgactgcc gacggctacg gcaacgacca
     3781 gacgacctac attgcctttg accgtccggc acaggggcag acctttgtga cgccttcggg
     3841 ctttcccaag taccggctgc gggcagttac cgtccgtcat gccggctata cggccaacac
     3901 cagcatcaca tggtaccgga tggatgccgg cagcaggctg acaatccgca ttaccaatcc
     3961 ggcgctggcc ggaacagccg gttttgtgct ggccagcgag ggctatgtcc tgaccggcac
     4021 cgagccgaac atcttttcgc ccaatacgat taccaacact gccaatggaa ccggcacatg
     4081 ggttaccttg accttagatg cgccggcaga attggcgccg aatactcttt acggctttga
     4141 tatcggctcc gattcggcct tttttgaaac gctgggcatc aaggagtccg tcagcaatcc
     4201 gtatccggcc ggctgggcgt acatcagcgg cgcttcggga gcgggcagca atgcctgcac
     4261 ggctgccgcc ggcgaccggg tgtttatcgt gcatctggac gccgtcatcc ccgatggggg
     4321 cgacctgacc ggcaacggca gggtggatct ggaagatgtg gcgctgctgt ccggcggctg
     4381 gcagcatctc tacggcctga cggcgctggc gcagatagcc gcccactggc tcttcaccga
     4441 gccaccggtt ttcaagtccg acccgattct cgccgccgat gcgcaggccg gcgccgctta
     4501 cagcgcttcg ctgaccgatg cggtcattta ctacaatccg ctgcggctgg tgtttgccaa
     4561 aatcagcggt ccggcgtggc tgacggtcag cccggagggc cgattgtccg gctgtccgtc
     4621 tgcggccgat gtgggcttgg ccagcgcggt ggtgcaggcc gacgacggga tgcacccgcc
     4681 cgtgcaggcg acggtgcaga ttgccgttgt ccaacagccg ctgcaggcgg ttccctttac
     4741 gcaggtaacc tttgccgatg cgttctggca gccgcgtctg cagaccaacc ggcaggtgac
     4801 cattccctac atcttcagca agcttcagga aaccggccgg attgacaatt ttgccatcgc
     4861 cggcaaactg atgaccggca cgccccgcta cgattttccg tttgatgata ccgacatcta
     4921 caagacgctg gaaggcgcca gctattcgct gatggttact cccgatccgg cgctggaggc
     4981 gtatctggat gagctgattg tcaaaatcgc cgccgcccaa gagcacaacg gctatctgcc
     5041 gggctatctg tataccatcc gcaccaatgg ggtggatatc tggtgcggcc ctgcgccgtg
     5101 gacgaatttg agcatgagcc atgagctgta caacgccgga catctgttcg aggcggccat
     5161 cgcccactat caggcaaccg gcaagacagc gctgctgaat gtggccaagc agtttgccga
     5221 tttgctggtg gacacctttc acgacggcgg ggtggagatt ccgccggggc atgaaattgt
     5281 cgaaagcgct ttggcgcggc tgtcggatac ggcggccgac aagcggtatc tggatttggc
     5341 gcggtattat ctggatatcc gcggcacggt gacggatgat tacagcccgt ggggccagta
     5401 ctatcaggac catctgccgg tgctccagca gaccgaagcc gtcggccatg tcgtgcgggc
     5461 ggtgtacctg tatatgggca tggccgatgt ggccgcacgc actgctgacg gcacctaccg
     5521 caaggccctg atgcaggcgc tggatgccat ctggcacagc gtcaacgata ccaaggccta
     5581 cataaccggc gggctgggcg cctcgcacgg cggggagtcg ttcggcgcac cgtactatct
     5641 gcccaacgac ggctactgcg aaacctgcgc ccagattgcc aatgtgatgt ggaaccaccg
     5701 gatgtttctg tatcaccgcg acggcaagta tatcgatgtg ctggagcgga cgctttacaa
     5761 tgccttaatc agcggcgtgt cgctgaaggg cgataccttc ttttatccca atccgctggt
     5821 ctccagcggc ggctacacgc ggagtgcgtg gttttcctgc aactgctgca tcggcaatat
     5881 tgcccgcacg attccgtcga ttcccggcta tgtatatgcc cgcggcgacg atgcgattta
     5941 catcaacctg tacgccgccg gcaccggaca ggtgcagctg gaccagacgg ccgtccgcct
     6001 gacgcagacc ggcaattacc cgtgggacgg aacaatgacc atcaccgtcc agccggatgt
     6061 gccggcggac tttaccattt atgtgcgaat ccccggctgg gcgcagaatc agccggtgcc
     6121 cggcgatttg tatgcctacc gcgatgccca gccgctgaca ccgagtctgg atgtcaacgg
     6181 cacgccggtt gcgctggccg tccagaaggg ctatgtcgcc gtcagccgca catggcaggc
     6241 cggcgaccag attcatctgt cgctgccgat ggccgtgcgg caggttcagg cccatccgaa
     6301 catcgccgcc gatgccggac gcatcgcact ggagcgcggc ccgattgtgt attgtgccga
     6361 atggcctgac tttgaaggcc gcacgattac gcatctgtac ataccggacg gtgcggattt
     6421 ttcggtcgag taccgcgaca atatgctggg cattcccgag ctgcccaatg ccggcgctgt
     6481 gatcaaaggc accgtcaaag gtctgtatga ggccgccggc ggcgccgtcg agcagcggga
     6541 tgtgccgttt acggccattc cctattatgc atgggcacac cgcggccccg gccagatggc
     6601 tgtctggctg cctgccggtc cggataaggc cgtgccgatt ccgcagccga cgcctgccga
     6661 gctggtcggc cattggaagc tggatgaaac cgccggcaca acagccgccg attccagcgg
     6721 caagcaaatg aacggaaccc tgctcaacgg gctgtcgttc agcagcaatg cggtggctgg
     6781 accggtcggc aatgccctca gctttgacgg cagcaatgat tatatcgatt tgccggacgg
     6841 cttcagcgat ttcgcaaagg gctgcacgat tacgctgtgg gcccggccga cggcggtaaa
     6901 gaactgggcg cggtttgtcg atctgggcaa cggttcggct gcggacaata tctggttcgg
     6961 ccggcagggc accaccggca atctggcctt tgagtgctgg gccggcagca gttccagcgg
     7021 actggtcacc gccgccggag ccatcgcctt gaacgaatgg cagatgtttg ccgttaccgc
     7081 cgacagtttc ggccatgtca agctctttaa aaacggccag ctgattcaga cgggctcctc
     7141 tgctccgtcc agcgttacac gcacccggaa tttcatcggc cgcagcaact ggtcggccga
     7201 tgcctactat cagggggctc tggatgatat ccgtatctac aactacaacc tcagcgacga
     7261 tgctgtcatg cagctgtaca ccggcttata ggaagctgct gatgcgggag aatatgaaat
     7321 gactgcgttc aaagaacgct gctggatgcg attgctctgc gggatgctcg ccgtgtcgtg
     7381 ggcggctgcc gacatcacct atacctttca tctggacggg gtggatgccg gcatagccaa
     7441 cacaatcaag gcctctgtag cggaagccgt gggcttgtat aaccagcacg gctcattcaa
     7501 taagcacctg tatatctact acaatgcggg tgttccgacg gcgcaggcca attacgacgg
     7561 cgtcatcacc ttcggcggct cgcggaacac gcgggtggca ctgcacgaga tggctcatac
     7621 attgggtacc ggcaccgtgt cggcgtggag cgccaatttg tccggcggcg tctggcaggg
     7681 ctactacgcc gcgaagctgg ccttggcgca gggcagcccc tatgctgacg gtctgcacgg
     7741 cgacggggcg catttctggc cgtgggggct caattacgac agcgaagacg gctttatgga
     7801 gcgcatttat catattcgca ccgttgctgc cctccgctgc gatatgggga ttctggcctt
     7861 tgcgctggag ccccggcatc agacggcaga tctgggcggc acggcggttt tctctgccgc
     7921 agcgccggcc gcggcaagct ggcggtggta caaggacgga gtcgccctga cagacggcgg
     7981 ccgcatcagc ggcgctgccg gacctgagct gcggattgcc caagcagagt tgaccgatga
     8041 gggcagctat tcctgtgccg caaccggtgc aggcgaaacg ctcttcagcc ggccggcccg
     8101 cctgacgata cgaaaactgg ccgcctatta caaattcaac ggcaatgcca acgacagtgc
     8161 cgcttccaac catgcggctg cattcggcag tccgttgtac agtgccggaa tggccgggca
     8221 ggcgatcgat ttggacggca ccgatgacta tgttatgctg ccggccggca tcgccgatac
     8281 cgatgatata acagtcggcg gctgggtgaa ctgggacggc ggcggaaact ggcagcggat
     8341 ttttgatttc ggcaccggca cagcgcagta tctgttcctg acgcccagct cggacagcaa
     8401 taggctgcgc tttgccatca aagacagcgg cggcgaacaa attgtggaaa ccgcccgtct
     8461 ggcggtcggc cagtgggtgc atctggcggt ggtgctgcag gatacgacgg ctgtgctgta
     8521 tgtcaacggc aggccggccg cccacagcgg gactgtgacg attgacccgc gcaatttcgc
     8581 gccgaacaga aactacatcg gcaaaagcca gttcagcgcc gatccgctct ttaacggccg
     8641 catcgatgat ttccggattt acacctgtgc ccttgacggt gcggacatct ggaactggtg
     8701 ggggcaaagt gccgacagcg ccccccggtt tgcagccgac ccgctgattc tgcccgacgg
     8761 cgctcagggg acggcttata ccggccagac gctggcggca tgggccgaag atgccgacgg
     8821 cgatacgctg actttcagca aaatcagcgg accggcttgg ctgtcggtca gcgccggcgg
     8881 cctgctttcg ggaacgcccg gcagcaccga tgccggcaag aatacattca ttgtccgtgc
     8941 cgcagaccca ttcggcgcct gcaccgatac ccaactcata atctatgtgc acggactggc
     9001 cgcagcgcat tatccgtttg aaggcaatgc cctcgaccaa tcgggcggcg gctggaacgg
     9061 aacagcaagc ggcgggccgg cctacacaag cggctttttc ggacaggcaa tcgatttgga
     9121 cggcatcgac gactatgttg tgctgccggc aggcatcatt cgcacagccg attttacggt
     9181 cgcggcatgg gtctattggg acggcggcgg cagctggcag cgcatctttg acttcggcaa
     9241 caacaccacg cggtatatgt tcctgacgcc aagctcaccc agcggcacgc tgcgttttgc
     9301 agtgaccgcc gccggcaacg gcagggaaga acgcgtcgaa acagccgctc tcacaccggg
     9361 ggcgtggacg catgtggcag tttcacgaag cggccaaacc cttgcgatgt atgtcaacgg
     9421 cacacgccgt gcggtcaatc cggtatcaac ggtcagtccg gctgattttg ctccggcgta
     9481 taactacatc ggcaagagcc aatggccgga tcctctcttt aacggccgga ttgacgattt
     9541 tcggatttac aactatgccc tgtcgccggc ggaaattgcg gtgctgagca gcacttcaat
     9601 ctttaccgat gaacagtttg ataacgatca ggcagcagaa cttgctcctt atgcaggccg
     9661 gccgctgacg gcgtttaccg attatgccgg cggctccggt gcgcttgtct ttgccaaagt
     9721 cagcggcccg gcgtggctgt ttgtttcccc cgacggcacg ctgtcgggca ttcctgccga
     9781 cc
//
LOCUS       DAOTBO010000100         9775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5918_length_9775_cov_5.005453, whole genome shotgun sequence.
ACCESSION   DAOTBO010000100 DAOTBO010000000
VERSION     DAOTBO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9775
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5918_length_9775_cov_5.005453"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLE88_10375"
     CDS             complement(<1..350)
                     /locus_tag="PLE88_10375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOM76910.1"
                     /translation="MACTKVQMKTTLGDMLIELDADAAPVTVKNFLEYVNDGFYDGTV
                     FHRVINGFMIQGGGMTKDLRPKSSHDPIINEASNGLKNKRGTIAMARTSDPDSATNQF
                     FINQKDNHFLDYQGA"
     gene            complement(384..2018)
                     /locus_tag="PLE88_10380"
     CDS             complement(384..2018)
                     /locus_tag="PLE88_10380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOM76911.1"
                     /translation="MKMKKVCVFLFLAAAMLCSAQSEVSLRWSSQKAQDWYNSQAWVV
                     GCNFTPSTAINQLEMWQEETFDPQTIDRELGWAAGIGMNTVRVFLHDLLWQQDSEGFL
                     RRIEQFLVIADRHKIKPMFVLFDSVWDPYPQLGKQRPPKPHLHNSGWVQGPHIDILKS
                     PEKIETLRPYVTGILERFKNDSRVLMWDLYNEPGNNNVPAYSELEPQNKDELGLYLLQ
                     KVFAWARQVNPSQPLTAGVWKNEWHPDKADTLNKYSLENSDIITFHDYSPLSRTKERV
                     ESLKPYGRPLICTEYMARAAGSTFAEQLPYFKDNRIGAINWGLVAGKTQTQYPWESWR
                     RQFTAEPEIWFHEVFRSDGTPYSPEETELIKKLTGQIPDSGEAGKAKNPIVRMKTSKG
                     DIQIELYADKAPITVKNFLDYVHSGFYNGTIFHRVIPNFMIQGGGFTPEMQQKPTNAP
                     IKNESYNQLRNERGTIAMARTNAPDSATCQFFINHVDNRSLDFDGPMKPGYAVFGKVV
                     SGMNVVDAIAAVPTKRVGPHSNVPADTIIIESIAAE"
     gene            complement(2111..2740)
                     /locus_tag="PLE88_10385"
     CDS             complement(2111..2740)
                     /locus_tag="PLE88_10385"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOM76912.1"
                     /translation="MSDKTMHVLAYFVLTFLIWFAVSPYDKIRWDKLKTWIVIAIITV
                     YAALDECLQGMIMGRSSDIIDFLSDILGMAAGVVVLMLFDFWPAMLTASAVFIFVVST
                     MSNLPHIYPKYHLDSIFHFTAYAAFTFIWIQHQRRSVFFRRFNMLFALIIPVFLLLAV
                     ITAARFWGRSISSAGIFAAIAGICLVLFSAGAACLIKSRLKKNANKDFQ"
     gene            complement(2894..3880)
                     /locus_tag="PLE88_10390"
     CDS             complement(2894..3880)
                     /locus_tag="PLE88_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76913.1"
                     /translation="MEQIQAGNIGILEPIGAAIEKTREILFHPFDVVKWLTIGFCAWL
                     ASFGSCGPGFNYQLNKFDNIQDLQNKILHLKDVVLSNLPVILSVGTAVAVIVLAISAL
                     LLWLHSRGQFMFLDCVARNSAHVVYPWKEYADQANSLFVFNIVLWLVGLATSAVFLVP
                     TVFLGVVMFIKGFAFVMHLFWIVLLVSGFIFVLMTIGLIKLMTKDFIIPIMYLRRCSA
                     LAAWREFKTLLFGYMWPFTLYLSFLFVVGIILFVIVLAAIFATCCCAACILAIPYIGT
                     VMMLPLLVWRRAYSLCYLAQYGSAYDVFGLQQITACNSVNQPTSCSQGPVDE"
     gene            complement(3926..4693)
                     /gene="hisF"
                     /locus_tag="PLE88_10395"
     CDS             complement(3926..4693)
                     /gene="hisF"
                     /locus_tag="PLE88_10395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOM76914.1"
                     /translation="MLTKRIIPCLDVKDNRVVKGVNFLNLRDAGDAVELGARYSEEGA
                     DELVFLDITATIRSRKTIVELVEKVAQNVFIPFTVGGGISKADEIGLLLRSGADKCSV
                     NTAAVSNPDLLKESAERFGSQCVVLAIDARRSRIRAGHWQVCVKAGSEPTEIDAVQWA
                     VRAEKLGAGEILLTSMDADGTQSGYDIELTRAVAESVGIPVIASGGAGTLESLAEVLE
                     KGKADAVLAASIFHYGTYTIKQVKDFLHQRGIPVRPI"
     gene            complement(4687..5310)
                     /gene="hisH"
                     /locus_tag="PLE88_10400"
     CDS             complement(4687..5310)
                     /gene="hisH"
                     /locus_tag="PLE88_10400"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOM76915.1"
                     /translation="MLVIIDYGVGNVRSVTNAFRYIGSDITVSCNPAIIRKAAGLILP
                     GVGASGYAMQRVTPIADIIKEQAAAGKPLLGICVGFQLLFDESHEMGVHRCLGLIKGK
                     VIPIPQDGGLTIPHMGWNYVTFTPEMRLFKHMHEGRHFAFANSYYAQVNDPLTTTAYT
                     EYGVLIAAAVEKGNIYGCQFHPEKSGTDGLQVLRNFVSICRERLSSC"
     gene            complement(5440..6204)
                     /locus_tag="PLE88_10405"
     CDS             complement(5440..6204)
                     /locus_tag="PLE88_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM76916.1"
                     /translation="MSHHIVQACDLHYVYPDGTIGLAGVNFRISHGESVALVGANGAG
                     KSTLLLHLSGCLLPTKGSVQIGDWAVTSRTRAAVRRSVGMVFQDSDDQLFMPTVFEDV
                     AFGPMNLGFGREEVIPVAMKALERVGISQLKDRPPYKLSAGEKRRAAIAAVLAMSPDV
                     LLMDEPSSSLDPRSRRELIALLQTFEHTKIIATHDLDLAIELCPRTIVLNQGRIIRDG
                     PTLTVFTDDELLAQSRLEKPLSMQNCPLCSSKKPRQ"
     gene            complement(6201..7010)
                     /gene="cbiQ"
                     /locus_tag="PLE88_10410"
     CDS             complement(6201..7010)
                     /gene="cbiQ"
                     /locus_tag="PLE88_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOM76917.1"
                     /translation="MKTETILHNLGYMDTLAAGDSWIHRLDPRAKLLAAGVFIGMVMS
                     FDKYQIMPVLSFMAIPIILTAISRLPTLYLIKRMLWVAPFAVMFAVFNPLLDRQILLQ
                     IGCLQISGGVVSFASIMLRFCLTVLMVLILVGTTGFYMLCLAMEKLAVPEIFVLQLMF
                     LYRYGFVLAEESGRMLRAFYLRAPLKQTVSIPVFSKLLGTLFLRTTARSQRIYQAMYC
                     RAFDGSLRLSRQLRWTNADTLFLLSITGLCMLFRFTNPPMQLGKLITGILL"
     gene            complement(7123..8130)
                     /locus_tag="PLE88_10415"
     CDS             complement(7123..8130)
                     /locus_tag="PLE88_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOM76918.1"
                     /translation="MHMADALISPAVGGTFWAISAGLIGYSCRQIRTKLDDSKIPLMG
                     VTGAFVFAAQMINFAIPATGSSGHLGGGMILAILLGPWAGFLVMASILTIQALFFADG
                     GLLALGCNIFNLGFWTCFVAYPFIYKKIAAQQPSQTKLFWASIISAVLGLQLGAFSVV
                     MQTVLSGISELPFGTFIMTMLPIHLAIGIVEGFVTAGVVRFIAKTNPQIIYTIQPQAK
                     NASLSVKYMGLILAAVIIGGMLSWFASSAPDGLEWSMLKTAGREELEPLAHPLYDKLD
                     SIQEKTAFLPDYSFKQVPEEKSQTEPKWPAVSAETTISGLVGVVSTLVLSAGIGFLLK
                     W"
     gene            complement(8166..9119)
                     /locus_tag="PLE88_10420"
     CDS             complement(8166..9119)
                     /locus_tag="PLE88_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOM76919.1"
                     /translation="MHSEGTWPRRDINDTSVGSAFGVNGDFAPREAFNVIELWYQQTC
                     FDDTLQIRLGKIDMTGGFECRGCPVSFDGNMYANDENTQFLNSALVNNPTIPFPDYGL
                     GAIIHWNPTENWYLSAGTADAQADKRETGFNTAFHQEDYFIYMAETGITPQIDSPKGP
                     LQGAYRLGVWYDPQPKLQSDSDKKYRDDAGVYISCDQKVTRENPEDMQGLGIFFRFGY
                     APSRSNDIAEFYSGGLQYQGLFCGRNEDILGLGFAYGAFSDSADTAFTEDYESAAELY
                     YSAVLTKWMTITPSIQYITNPGGDKSGSDAIVLGLRALIAF"
     gene            9114..9419
                     /locus_tag="PLE88_10425"
     CDS             9114..9419
                     /locus_tag="PLE88_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76920.1"
                     /translation="MHINRAALYIQQAFQIANGYDAAADEADKKQKKAERDADLQPFT
                     MVDTLIRQNASDRASLKAAKLADVKDLLDGLYVREALILKALSRMQEGQSISQGDGQ"
     gene            9416..9739
                     /locus_tag="PLE88_10430"
     CDS             9416..9739
                     /locus_tag="PLE88_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76921.1"
                     /translation="MKIRSVLLLVMVLVLMNVMVVGAQPAHACGTITALSVSFDRVDM
                     SGMPFAPRLKEGDLTQFNRIAARDPYFNGQRFDASYKAANYWFGLLGQELGVSMIQLP
                     PPSTP"
BASE COUNT         2778 a         2414 c         2091 g         2492 t
ORIGIN      
        1 gccccctgat aatcgagaaa atgattgtcc ttttggttga taaaaaactg attggttgca
       61 ctgtcagggt ctgatgttcg ggccattgca attgtgccgc gtttgttctt cagaccatta
      121 gaggcctcgt taataatcgg atcatgactg gatttgggac gcaggtcttt ggtcattccc
      181 ccgccctgta tcataaaacc gttaatcact cgatgaaaaa ctgtaccgtc ataaaagccg
      241 tcattaacat attcgagaaa atttttgacc gttaccggcg ccgcatcggc atccagctca
      301 ataagcatat cgcccaatgt tgttttcatt tgaacttttg tacaagccat acaatcccca
      361 ttttcaagat agaagaatta tctctattct gccgcaatag attctataat aatagtatca
      421 gccggaacat tggaatgagg gcctacgcgt ttggtaggta cagccgcaat cgcatctacc
      481 acattcattc cgctaacgac ttttccaaaa acagcatacc ccggcttcat cgggccgtca
      541 aaatccagcg aacggttatc aacatgattg atgaaaaact gacaggtagc gctgtcgggt
      601 gcattggtac gggccattgc aattgtaccg cgttcgtttc gcaactgatt ataagactca
      661 tttttgattg gtgcattagt gggcttttgc tgcatttcag gggtaaaacc gccgccttgt
      721 atcataaaat taggaataac gcggtggaaa attgttccat tatagaaacc tgaatgtaca
      781 taatccaaaa agtttttgac agtaataggt gctttatcgg catacaattc aatctgaatg
      841 tcgcctttgc tggttttcat ccgtacaatg ggattttttg ctttaccagc ctctcctgaa
      901 tcggggattt ggcctgttaa tttttttatc agctcagtct cttctggact gtaaggagtc
      961 ccatcagagc gaaatacttc atgaaaccaa atctccggtt cggccgtaaa ctgcctgcgc
     1021 caagattccc aagggtattg tgtttgagtt ttgccggcaa ccaagcccca gttgatcgct
     1081 ccgatgcgat tgtccttaaa ataagggagc tgttctgcaa atgtggaacc tgctgctcga
     1141 gccatatatt ctgtacaaat cagcggccgc ccgtagggtt ttaagctttc cacacgctct
     1201 ttcgtgcggc tcagagggct gtaatcatgg aacgtaatga tatcggagtt ttcgagtgaa
     1261 tatttgttaa gggtatcggc tttatccgga tgccattcgt ttttccaaac tccagccgtc
     1321 aacggctgtg agggattgac ctgacgggcc catgcaaata ctttctgaag caaatagagt
     1381 cccagctcat ctttattttg cggctccaat tcggaatatg cgggaacatt attgttaccg
     1441 ggttcattat acaaatccca catcagaaca cgggaatcat ttttgaatct ctccaaaatg
     1501 cccgttacat atgggcgcag agtctctatt ttttcagggg atttgaggat atcaatatgc
     1561 ggaccttgca cccacccgga attatggagg tgcggtttgg gcggacgctg tttacccaac
     1621 tggggatagg gatcccagac gctgtcaaaa aggacaaaca tcggttttat cttatgtctg
     1681 tcagcaatca ccagaaactg ctctattcgc ctcaaaaatc cttcgctgtc ctgctgccac
     1741 agcaaatcat gaaggaacac ccgcactgta ttcatcccga tgccggcagc ccagcccagt
     1801 tctcggtcaa ttgtctgggg gtcaaaagtt tcttcctgcc acatttccaa ctgatttatg
     1861 gcggtgctgg gtgtaaaatt acagccaacc acccaagcct ggctgttata ccaatcctgc
     1921 gccttttggc tggaccaccg caatgacact tcagattgag cggaacacaa catagctgcc
     1981 gccagaaaca gaaaaacaca cacctttttc atcttcattt gtctcctgtt ttcaactctt
     2041 ctttctttgg acgacaaata atatacccgt catttgttct tttgccagtt atttcttaca
     2101 aggtccgtct tcattgaaaa tctttatttg cattcttttt taatcttgat tttatcaaac
     2161 aagccgcacc cgctgaaaac aaaaccaaac agatcccggc aatggcagca aatatgccgg
     2221 ctgagctgat acttcgtccc cagaatcttg ctgcggttat gacagccagc agtagaaaaa
     2281 caggaataat tagcgcaaac agcatgttga aacggcgaaa aaatacgctg cgtcgctgat
     2341 gctgaatcca gataaaggta aaagcagcat aagcagtaaa atgaaaaata ctatccaaat
     2401 gatatttcgg ataaatatga ggaagatttg acattgtgga aacaacaaaa ataaataccg
     2461 ctgaggccgt cagcatagcc ggccaaaaat cgaacagcat caataccacc acacctgccg
     2521 ccattcctaa tatatccgat agaaaatcga tgatatccga ggaacgcccc attatcatcc
     2581 cctgcagaca ttcatccaat gcagcataaa ctgtaatgat ggctattact atccaagtct
     2641 tcagcttgtc ccagcgtatc ttatcgtaag gactgacagc aaaccaaatc aggaaagtca
     2701 atacaaaata cgccagcaca tgcattgtct tatcactcat tccgctcctg cgagcgatct
     2761 caggcacagg aatatgcgtc agccagaaaa tggccggcca ataaattcca agagctgtta
     2821 aaactgattt gtggcggcga agtaaatgca tcagcgtttt acggcgcaaa atggcttctc
     2881 atggaagtaa tatctattca tctacaggcc cttgcgagca gctggtgggc tggttcacag
     2941 aattgcatgc tgtgatttgc tgtaggccaa aaacatcata cgcagaaccg tactgtgcca
     3001 gatagcacag tgaataagct cgccgccaca ccagcaaggg cagcatcatg acagttccaa
     3061 tatagggaat tgccagaatg catgctgcgc agcagcaggt agcaaatatg gccgccaaca
     3121 cgattacaaa taaaataatg cccacgacaa ataaaaaaga tagatacagc gtaaacggcc
     3181 acatatagcc gaataaaaga gttttaaatt cccgccaagc agcaagagcg gaacatcgcc
     3241 gaagatacat aatagggatt ataaaatctt ttgtcatcaa tttgataagg ccaatagtca
     3301 taagcacaaa tataaatcct gataccagta gaacaatcca aaacagatgc atcacaaaag
     3361 cgaatccctt aataaacatc accacaccca gaaaaactgt tggcacaaga aaaacagccg
     3421 acgttgcaag gccaaccagc cataagacaa tattgaacac aaacagactg ttggcctgat
     3481 ctgcatattc cttccatgga taaacaacat gggcactgtt cctggctaca caatccaaaa
     3541 acataaattg cccccggctg tgcagccaca gaagcaaagc cgatatggca agaacaatga
     3601 ctgctacagc cgttccaact gaaagaataa ccggcagatt actaagaacg acatctttta
     3661 gatgcaggat tttattttgc agatcctgta tgttatcaaa tttgttcaat tgataattga
     3721 atcccggacc gcagcttcca aaggaagcca gccatgcaca aaatccgata gtaagccatt
     3781 ttacgacatc aaaaggatgg aagaggattt cccttgtttt ttcgatagcc gctccaattg
     3841 gctctaatat gccgatattt ccggcctgta tctgttccat tgccaacctc tctttctaat
     3901 gatcatgccg tgcctaatag cattattata taggacgcac aggaattcct ctctgatgca
     3961 gaaaatcttt aacttgtttg atagtgtaag taccatagtg aaaaattgag gccgccagta
     4021 cagcatctgc tttgcctttt tcaagcacct cggccaaaga ctccagcgtt ccagctcccc
     4081 cagaggcgat taccggaata cccacggact cggcaacagc ccgagtcagt tctatgtcat
     4141 agccggattg tgtgccatca gcatccatac tggtcaaaag aatctcgccg gcaccaagtt
     4201 tttcagcccg tactgcccat tggactgcat caatttctgt aggttcagaa cctgccttta
     4261 cacacacttg ccaatggccc gcccggattc tgctccggcg ggcatcaatt gccagaacta
     4321 cgcactggga tccgaatcgc tcggcggatt ctttcaataa gtctggattt gatacggcag
     4381 ctgtattaac cgaacattta tccgctccgc tccgcagcaa aagacctatt tcatcggctt
     4441 ttgatattcc ccctccgacg gtaaagggaa tgaaaacatt ctgggctact ttttcgacaa
     4501 gttcaacaat ggtctttctc gaccggatag tagctgtaat atccagaaat acaagttcat
     4561 cggccccttc ctcactgtag cgggcgccta attccacagc atcgccggca tcccgcagat
     4621 tcagaaagtt tactcccttg acaacacggt tatctttcac atccaggcac ggaatgatgc
     4681 gctttgtcag catgatgaaa gcctttcacg gcaaatagag acaaaatttc tcaatacctg
     4741 caagccatca gtgccgcttt tctcggggtg gaattggcag ccgtaaatat tgcctttttc
     4801 aacagcggca gcaatcagaa caccatattc tgtataggca gttgtggtca gcgggtcatt
     4861 tacttgtgca tagtatgagt tggcaaaggc aaaatgtctg ccttcgtgca tatgcttaaa
     4921 cagccgcatt tctggtgtaa atgttacata attccatccc atatgtggaa tagtcagccc
     4981 tccatcttga ggaataggaa taacctttcc ttttatcaat ccaagacagc gatgcacacc
     5041 catttcatgg ctttcatcaa ataaaagctg aaagccgaca cagatgccca gcagcggttt
     5101 gcctgcagcc gcctgttctt ttattatatc ggcaatagga gtcacccgct gcatagcata
     5161 tccgctggcc ccgaccccgg gcagaatcag accggccgct ttgcggataa tggccggatt
     5221 gcagcttacc gttatatctg acccaatata acgaaaagca tttgttacgc tccgcacatt
     5281 ccctacacca taatctataa taaccagcat atatgacaat ttcctgcaga tttaacggat
     5341 tatttctgat atacccgtat ttctaattat actaggatga ccatgattta tcaactcgtt
     5401 ttgtcggaaa gcaggcggtt caagaaagac agactagtct tattgtcgcg gcttctttga
     5461 gctgcacaag ggacagtttt gcattgatag gggtttttca aggcggcttt gggccagcag
     5521 ctcgtcatct gtaaaaacag tcagagtggg accatccctt ataatccggc cctgattgag
     5581 aacaatagtg cgtggacata attctattgc caaatccaga tcatgggtgg cgatgatttt
     5641 tgtatgctca aaggtctgca gcagggcaat cagttcacgc cggcttcgag gatctaagct
     5701 ggatgacggc tcatccataa gcaaaacatc cggactcatc gccaataccg cggcaatagc
     5761 agcccggcgt ttttcacctg cagacagttt ataggggggg cggtccttta attgcgaaat
     5821 acccacacgc tccaaggcct tcattgcaac aggtatcact tcctctctgc caaatcccag
     5881 attcattggt ccaaaagcaa catcctcaaa tactgtcggc ataaagagct ggtcatcact
     5941 atcctgaaaa accattccga cagaacggcg cacagcagca cgcgtgcggg atgtaaccgc
     6001 ccagtcccct atttgcaccg atccttttgt cggcagcaga cagccactaa gatgaagaag
     6061 caaagtggac ttgcccgccc cattggctcc aaccagagct acagattccc catgtgaaat
     6121 tcgaaaattt acccccgcca atccgattgt tccatccgga taaacataat gcaaatcgca
     6181 ggcctgaaca atatggtgac tcacagcagg attcctgtta tcaattttcc tagctgcatc
     6241 gggggatttg taaaacgaaa gagcatgcaa agcccagtaa tacttaataa aaacagcgtg
     6301 tcggcgtttg tccatcgcag ttgtcgggat aaacgcaggc tgccatcaaa ggcccgacaa
     6361 tacatggcct gataaatacg ctgagagcgg gctgtggttc ttaaaaaaag agttcccagc
     6421 agctttgaaa aaaccgggat acttactgtt tgttttaaag gtgcccgcaa gtaaaatgca
     6481 cgcagcatcc gtccgctttc ctctgccagc acgaaaccat acctgtacaa aaacatcagc
     6541 tgcaatacga atatttcagg caccgcaagt ttttccatgg ctagacacag catataaaat
     6601 cctgttgtgc ctacaagaat aaggaccatt agcacggtaa gacaaaagcg aagcataata
     6661 gacgcaaagg aaactactcc gcctgagatt tgcaaacatc caatctgcag caggatctga
     6721 cggtcaagca gcggattaaa gaccgcaaac atcacggcaa agggggctac ccatagcatt
     6781 cgcttgatta aatacagcgt cggtaggcgg cttatcgccg ttaaaataat cgggatcgcc
     6841 ataaatgaca aaacaggcat aatttggtac ttatcaaagg acataaccat cccaataaac
     6901 acaccggccg ccagcaattt tgcgcgcgga tccagacgat gaatccagct gtctcctgca
     6961 gccagagtat ccatatagcc tagattatga agaatagttt ctgtcttcat tatctatatt
     7021 atgcttttca tacaaaacac tttcaccggt ttatatccga aaaaggtatt tataaaagat
     7081 tccggagttt tatatatttc gttttagact cgagggttta agttaccatt tgagcaaaaa
     7141 gccgatccct gcagacaaaa caagcgtcga aaccacccca accaacccgg agatggttgt
     7201 ttcggcactg acggcaggcc atttcggctc tgtctggctc ttttcctccg gtacttgctt
     7261 gaagctgtaa tccggcagaa aggcagtttt ctcctgaata ctgtctagtt tgtcgtaaag
     7321 agggtgtgcc aacggctcca actcttcgcg gccggcggtt tttaacattg accactcaag
     7381 tccatcgggg gcagaagatg caaaccaact taacattccc ccgataataa ccgcagccaa
     7441 tatcaatccc atatatttga ctgataatga cgcgtttttt gcctgcggct gtattgtata
     7501 tataatctgc ggattggttt ttgcgataaa tctcacaaca ccggccgtta caaatccttc
     7561 aacaattcct attgccaaat ggataggcag cattgtcatt atgaaagttc caaatggcag
     7621 ttctgaaatg ccggataaaa ccgtctgcat aacaacgctg aacgctccca gctgcaatcc
     7681 caaaaccgca gaaattattg atgcccagaa taacttagtc tgagacggct gttgtgccgc
     7741 aatcttttta tagatgaaag gatatgctac aaagcatgtc caaaatccaa gattgaagat
     7801 attacatccc aaggccaaaa gacctccatc ggcaaaaaag agagcttgga tcgttaaaat
     7861 agatgccatc accaaaaagc cagcccaagg tcccagcaat atcgccaaaa tcattccgcc
     7921 gcctaaatgt ccgctggaac ctgttgccgg tattgcaaaa ttaatcatct gcgctgcaaa
     7981 aacaaatgcc ccggttactc ccataagagg aattttgctg tcgtccagtt tggtgcggat
     8041 ttgcctgcag ctgtatccga ttagtccagc ggaaatagcc cagaaagtcc ctcctactgc
     8101 cggtgaaatt aatgcgtctg ccatatgcat atcaattttc tccttttaag caggtttgtt
     8161 accgcttaaa atgcaattaa agcccgcaac cccagcacaa ttgcatcgct tccagatttg
     8221 tcgccgccgg gattggtaat atattgaatg gatggagtta tcgtcatcca tttggttaga
     8281 actgcgctgt aatacaattc tgctgcgctt tcataatcct ctgtgaaggc agtatcagca
     8341 gaatcgctga atgccccata agcaaagccc agtcccaata tatcctcatt gcggccgcag
     8401 aacagaccct gatactgcag gcccccactg taaaattcgg caatatcatt gctgcggctg
     8461 ggagcatatc caaaccggaa aaatatgcct agtccctgca tatcttcagg attttccctt
     8521 gttaccttct gatcacagct gatatatacg cctgcatcat cccgatattt tttatcggaa
     8581 tccgattgca atttgggctg gggatcatac cagacaccca gccggtaagc cccctgcaag
     8641 ggtcctttgg gggaatctat ttgcggagtt atgcccgttt cggccatata aatgaaataa
     8701 tcttcttgat gaaatgctgt attaaagcca gtttctcgct tatctgcctg cgcatcggcg
     8761 gtaccggctg aaagatacca gttttcagtc ggattccaat gtatgatggc acccaatcca
     8821 taatcaggaa acgggatggt tgggttgttt actaatgcac tattaagaaa ttgggtgttt
     8881 tcatcattgg catacatatt gccgtcaaac gacacaggac agccccgaca ttcaaaaccg
     8941 ccggtcatat caattttacc caagcgaatc tgcagcgtat catcaaaaca ggtttgctgg
     9001 taccacaatt cgataacgtt aaatgcttcg cgaggagcga aatctccatt tactccaaat
     9061 gccgaaccca cagatgtatc attaatatcc cgccgaggcc aagtaccttc tgagtgcata
     9121 taaatcgtgc cgccctgtat atccagcagg cgttccagat cgctaatgga tatgacgcgg
     9181 cggctgacga ggccgacaag aaacagaaga aggcagagcg tgacgcggat ctgcaaccgt
     9241 tcacgatggt ggatacgctg atccgacaaa atgcgagcga tagagcatcc ctgaaggccg
     9301 caaaattggc agatgtgaag gatttgctgg acgggttgta tgtgcgtgaa gcgctaatcc
     9361 tgaaggcact ttcccgaatg caggaaggtc aatctattag tcaaggggat ggacaatgaa
     9421 gatcagaagc gttttgttac ttgtcatggt tctggtgctg atgaatgtca tggtagtggg
     9481 ggcgcaaccg gcgcatgcat gcgggacgat cacggcctta agcgtgtcat tcgaccgggt
     9541 ggacatgtcg ggcatgccgt ttgccccccg gctgaaggaa ggcgatctga cgcagttcaa
     9601 ccgcatcgcc gcgcgtgatc cgtatttcaa cggccagcgg tttgacgcca gttataaggc
     9661 ggcgaattac tggtttggtt tactggggca ggaactgggc gtgagcatga tccaactgcc
     9721 gcccccgagc accccgtaaa ggatacttaa tgggcaacga aaacattttc accgt
//
LOCUS       DAOTBO010000101         9731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_5968_length_9731_cov_4.874328, whole genome shotgun sequence.
ACCESSION   DAOTBO010000101 DAOTBO010000000
VERSION     DAOTBO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5968_length_9731_cov_4.874328"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..542
                     /locus_tag="PLE88_10435"
     CDS             111..542
                     /locus_tag="PLE88_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76922.1"
                     /translation="MFVAYYCDPHSPTYGKPTASYIASGYKGNSYNGLRATAGKVLMR
                     PAVKAAIAAYASDLRRRAVADHDYIWRQWLALLEDCRDAAGRYTDRPTAAAVLRTMAQ
                     ARAMLTDRVEQTGPQSPELTDAERAELQRLAREHNLRLAAG"
     gene            875..2467
                     /locus_tag="PLE88_10440"
     CDS             875..2467
                     /locus_tag="PLE88_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOM76923.1"
                     /translation="MNDVIQRLSAVDCISWCVYNKLMVREGIPFSLDGTPYLVDLVHR
                     RERVVHIKKGAQVRCTTLKFLEQVHACLYGRYKQNVLYMMPTVKQVEALSRISFDPLL
                     DCNLWLRRYVSVNTAALKTIAGRSIYFVGAQPQRVGGTNTKDSPSLRSIPCDCVLRDE
                     LDLMDEDMVELSRQRIRDSELKLECNFASPTYPGYGIDACYERSTQYKWHIKCRSCGR
                     HTCLVEHFPDSIRRVDGRWRRSCVHCGAEVFVQDGHWEADYPDRPEAGFWVDGLLSPR
                     ADLEADMLRYHNSEGAARCEFMRSVLGIASVEANNQLTDKDIYERCCLDLMQYTSSVD
                     TVMGADVGDVLHYVVGVRTGTEHYTVLRVGTAEDFGHLHEVAKQMRVKLCVLDAMPDI
                     HASRKFQREEPYTVYRCHYSEQMPGEPKFDAKTGVVKCNRNEMCDKVYDLFASSRIRI
                     PRLSGDIKEYARQMTQTAKTLITNPDTGLAKPKWVKLGDDHYFHATLYFLLACMRSSV
                     VSLSDGHPSVRRFRRVKNTFYV"
     gene            2476..2661
                     /locus_tag="PLE88_10445"
     CDS             2476..2661
                     /locus_tag="PLE88_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76924.1"
                     /translation="MSSAFGGGGSVHVPEVKEPPQTVTEDKEEIRRRMQRKYANASRQ
                     ENLLYGISQVLKKRLGE"
     gene            2663..4270
                     /locus_tag="PLE88_10450"
     CDS             2663..4270
                     /locus_tag="PLE88_10450"
                     /inference="COORDINATES: protein motif:HMM:NF023657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HOM76925.1"
                     /translation="MEKSAIKTLLSRYAQAKTARQAWDNTFSEVARYVWPDACSVVRS
                     VPDESAEGRVLTVDISDSTAIKASVRMTSGIFGWMMPMGTKWFEFRPAKEQDANSRDV
                     QRRLAAASQVLHSEIWRSNFQREMFNTIRSMVVFGTGAISVEWLDDGLLFRSYPIGNI
                     FFEEDSKGRIDTVFRREFFTLRQMAQEFGADMLPPDKQRRLADTPDDNAKYEVVHCVF
                     PRTDYDITKVDAKGQRFASVWLDAEAEAVLRKGGFDSMPYRIGRFDRSPNELMGRSPA
                     IELLPDIKMLNSMRRTFVMSCELQSCPALIVEDDSVIGQPSTGPGDIIVKRHGADDPR
                     PLQTGANPQLTAEAIAVEREGIMQGFFMDMFDVLERYRNMTAYEASRRIEDKQVLIAP
                     MISGLQKEMLDPLILRSLDLISSKKRLAEPVSSEDGLQIVYQGRLSLAMSNLQASAIE
                     LTLAKWSPYQAMYPVLDNVNLDKSFVMSALNAGVPADCLRSQRELEQIRQQAQQVQQA
                     QQTVELAEKGSKAIRNLSGTSLEGLVL"
     gene            4267..4518
                     /locus_tag="PLE88_10455"
     CDS             4267..4518
                     /locus_tag="PLE88_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76926.1"
                     /translation="MNEIEVRNRKAKQAMDELSRAYKTVFSGQAGQRVLADISRFCGA
                     DAVSASESQPNALTTFFREGKRSVFLRIIWQLNRKESND"
     gene            4511..5323
                     /locus_tag="PLE88_10460"
     CDS             4511..5323
                     /locus_tag="PLE88_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76927.1"
                     /translation="MTELNAQTSQAAAQTASPAQGWVAGDGTFANHQMMPEDVRNVVL
                     SKKWSNVADIVKSYRELESFIGGHKSRLNIPDNLDDSALNEIYSKLGRPDTPDKYQLQ
                     AKVPEGMQMDNQLVERFKKFAHAKNLTTTQFNDLVQFQLDAAAEAAKADEQARLKQIE
                     EAAIALKQEWKDAYASNFSTAKHAAEKLGIMDELEKLGLADNPAVIRMLYKLGTKLSE
                     DTLGVRTNAPAMSREEELKKLLASPEFNDKMHPGNQAAFRRFLELHGLTGQA"
     gene            5416..6363
                     /locus_tag="PLE88_10465"
     CDS             5416..6363
                     /locus_tag="PLE88_10465"
                     /inference="COORDINATES: protein motif:HMM:NF040375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HOM76928.1"
                     /translation="MSDPNYMALFYNAYNAARLHVPQERKNPFEGTVTTDVLRGEQQS
                     YDDFGISNLKQKTTRFATVETTDNDMRRRWLFANWYYDAKLVDRQDNIALLSDPTGDF
                     IKSLAYGVERLKRGVILTAFEASVSGGKNPGGTSYSFSNTAIGSGGAVIVHDTTYAGA
                     AGGTSKGLSTDKLILIRETFSRWGVPDGVPIYLACTFRQKSDLLREQQSKGQNTSEVE
                     ALVDGRINRYMGINFVETNGITLTSNGDLDGDTSVFKCYAYIPEGIVLATSPAPNFAV
                     DKRAELVGDTWQIRADFAAAAIRRNENMVIKVECANTAS"
     gene            6388..6825
                     /locus_tag="PLE88_10470"
     CDS             6388..6825
                     /locus_tag="PLE88_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76929.1"
                     /translation="MATYYGVNSTQAKSSSMSGQISKLWHNPLMMVYDKYVVPTGGVA
                     AGSDIVMGTIPKGARVVGFIFQGDDNGSTTATGTIYLGTTAATASAAITDLASGPVAL
                     FIPALDTFSQTPLTSSTAVSIKTATGALAADVVLTLTTLYVMD"
     gene            6900..7637
                     /locus_tag="PLE88_10475"
     CDS             6900..7637
                     /locus_tag="PLE88_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76930.1"
                     /translation="MSLTDAKIAVCNRALGYIGEHWIDANNTSSKPYEMCDRYYDDAV
                     KEVLVEHAWNEAKTRSIVVQEADEPLFGYTYAFALPTDCLRVLRIGNGSMDTQDWEVE
                     GGKILTLSAQRPLDYVVGQTYQAGQYITYSDYTYSVHTSFTASSWETDSAEYCTSTGG
                     DYAVLQIEYIKNLTDTSMWSPKLLDAVCQRLAIKVAVGLSNNPRARYDLLEEFEKITM
                     RKARSIDAQQGRVKSFLKSAWIRARGL"
     gene            7637..>9731
                     /locus_tag="PLE88_10480"
     CDS             7637..>9731
                     /locus_tag="PLE88_10480"
                     /inference="COORDINATES: protein motif:HMM:NF027516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating E1 FCCH domain-containing
                     protein"
                     /protein_id="HOM76931.1"
                     /translation="MQTIKTTFNAGKLSAYLDGRVDISKYYNGCSQLVNAIPLSTGGI
                     TKRPGTRFIAKAKGPCRLIPFEFSADDTLVLEFSDLAVRFYKNCDRVMLPSTAISSIT
                     LLPDTEVAVDTTEAHGLQTGDVVRFADVLGTDELNYAGNFATEWTITKTSSTAFTLDG
                     TNGSNFSEYKGGGTVAGIYSIASPYTLDDVWQLHYTQSGDVLYLTHPDYPPYTLSRYG
                     DASWVIQEANLVGGPFLGENTTDTYLLGFTRTGGIARCGHYFPAGAIGTLTASGTGNA
                     PFLPQHVGSLWMLKHTRPDNTMSTPDNTTNAVPTGTGIKIKGSFSFDVTVNSNTFKIW
                     RRQGNGAWQEYRTFLANTAYTSDELDDDVYYTFTSSSSSGSAVLTAKDQTNVGIVKIT
                     GYISPTSVSATVIQPVLSNNSDDSAVTTSMWAEGAFNAYRGYPRTLVIHENRLWFAST
                     SNNPQTLWGSSSSRFLNFTAGDTDSDAVTVTIEDSDVSNIQWMASDEYLVVGTANKEY
                     KISASNIDDPITPDDVRCRVQSALGSNSMQPVHLNNTLFYFQGGGRTLRSMRLMDATL
                     RYESDNATLLVDDIFDSLPVQIAVQRVPYAILWIVRQDGALVSFTYEPKEEVSAWSEH
                     VTGGLLLTPYQGQFSSVCVIRGMPEDEVYVCVRRIIGGIEQYYIEQFAARYTGRQDTQ
                     SFLDCSTVFTANSQPV"
BASE COUNT         2268 a         2451 c         2804 g         2208 t
ORIGIN      
        1 caggcatgga ttagttacaa ttcaaagact ccgtctccgc agcagcagcc ggctacagtc
       61 cgctgctgcc tgacttgcca gagctcaact gcctgcagcc gcagattcgc atgtttgtcg
      121 cgtactactg cgatccccac tcgcctacct acggtaagcc gaccgccagt tacatcgcca
      181 gcggttacaa aggcaatagt tacaatggtt tacgggcgac ggccggcaag gtgctgatgc
      241 ggccggcggt taaggcggca atcgccgcct atgcatcgga tctgcggcgg cgggccgtgg
      301 ccgaccacga ctacatctgg cgacaatggc tggcgctgct ggaagactgc cgtgatgccg
      361 ctggccgtta caccgacagg ccgacggctg cggccgtgct gcgcacgatg gctcaggcgc
      421 gtgccatgct gacagaccgc gtcgagcaga ctggcccgca gtcgccggag ctcactgatg
      481 ccgagcgtgc cgagcttcag cggctggcgc gcgagcacaa tcttaggctt gctgccggct
      541 gaccgtcggc aagcaggctg ccggcaggcc ggcaggccgg caggcaggcc ggcaggccgg
      601 ccggcaaggc ctccagcggg ccacaggatt agcgattggc tttggccggc acaaagacac
      661 cttgagccgg cgttcggcct tctggcgggc tgccagaggc cttaaaagcc aaaggggggg
      721 gggcaaagca aggggctttt ttgcaggggg ggaggcaggc ggacaagggg gtggggggat
      781 gcaggcagta cctatccacg tccggacttt taggcttttc agcaatagga caaggcttat
      841 cggcaataga cctaaaaaag aagaagaagc gagtgtgaat gatgtaatcc agcggctgtc
      901 ggctgtggac tgtataagct ggtgtgtata caacaagctg atggttaggg aggggatacc
      961 tttttcgctg gatggcacgc cgtacctggt tgaccttgtg caccgcaggg agcgtgtagt
     1021 tcacattaag aagggggcgc aggtgaggtg cacgacgctg aagtttttgg agcaggtgca
     1081 tgcctgctta tatgggcgat acaagcagaa tgttttgtac atgatgccga ctgttaagca
     1141 ggttgaggct ttatccagga taagttttga cccccttttg gattgtaatt tatggcttcg
     1201 gcggtatgtt tctgtcaaca cagcggcgtt gaagaccata gctggccgga gcatttactt
     1261 tgtcggcgct cagccccagc gtgttggcgg gacgaacacg aaggattcgc cgagtctgcg
     1321 ttcgattcct tgcgattgtg ttttgcggga cgagctggat ttgatggatg aggacatggt
     1381 tgagctgagc cgtcagcgga ttcgggattc cgagctgaag ctggagtgca attttgcctc
     1441 gccgacttat ccaggttacg gcatagatgc ttgttacgag cgttcgacgc agtacaaatg
     1501 gcatatcaag tgccgttcct gtggcaggca cacctgtctt gtggagcatt ttccggactc
     1561 cattcgcagg gttgacggcc gctggcggcg cagctgtgtg cactgcgggg ctgaggtttt
     1621 tgttcaggat gggcactggg aggctgatta tcccgaccgt ccggaggcgg gcttttgggt
     1681 ggacggcttg ttgagtccac gtgctgacct tgaagctgac atgctgaggt atcacaacag
     1741 cgagggcgcg gcccgatgtg agtttatgcg gtctgttttg ggcattgctt cagtagaggc
     1801 caacaaccag ctgactgaca aggatattta tgagcgctgc tgtctggatt tgatgcagta
     1861 tacctcgagt gttgacactg taatgggtgc agatgtcggc gatgtgcttc attatgttgt
     1921 aggcgtgcgt acgggcacgg agcattacac ggttttgagg gtcggcacgg ctgaggattt
     1981 tgggcatctg cacgaggttg ccaagcagat gagggtaaag ctgtgcgtgc tggatgccat
     2041 gccggacatc cacgcttccc gcaagtttca gcgggaagag ccctacacgg tatatcggtg
     2101 tcattattcc gagcagatgc ctggagagcc caagtttgat gccaagacgg gggttgtcaa
     2161 gtgcaaccgc aatgagatgt gcgacaaggt ttatgatttg tttgcttcca gccggattcg
     2221 gattccacgt ttatccggcg acattaagga atacgcccgt caaatgaccc agacggccaa
     2281 gacattgatt accaaccccg acaccggttt ggccaagccg aagtgggtaa agctgggcga
     2341 tgaccattat tttcacgcca cgctttattt tttactggcg tgcatgcgga gttctgtggt
     2401 ttctctgtcg gacgggcacc caagcgtccg caggtttcgc agagtcaaga acacctttta
     2461 tgtataggag gccttatgag cagtgcattc ggcggcggcg ggagcgttca tgttccggag
     2521 gtgaaagagc ccccgcagac agtaactgag gacaaagagg agattcggcg gaggatgcag
     2581 cggaagtacg caaacgccag cagacaggaa aaccttttat acggcatatc gcaggtgcta
     2641 aagaagcggt tgggcgagta agatggaaaa atctgcaatc aaaacactgt tgtcccgtta
     2701 tgcccaagcc aagacagcac gccaggcctg ggataatacc ttttcagagg tagctcggta
     2761 tgtctggccg gatgcctgct ctgtagtgcg gtctgtgccg gacgaatctg ccgaaggcag
     2821 agtgctgacg gttgacattt ccgactcgac cgccattaag gcttccgtgc ggatgacctc
     2881 cggcattttt ggctggatga tgccgatggg cacgaagtgg tttgagttca ggccggctaa
     2941 ggagcaggat gccaactcca gagatgtcca gcgtcgtctg gcggcggctt cgcaagttct
     3001 gcattcagag atatggcgga gcaacttcca gcgggagatg ttcaacacca tccgctcgat
     3061 ggtggtgttc ggcacaggcg ccatatcagt agaatggctg gatgacgggc ttttgttccg
     3121 cagctatcca atcggcaaca tcttctttga agaagactcc aagggccgga ttgacactgt
     3181 ttttcggagg gagtttttca ccctgcggca gatggcgcag gagtttggtg ctgatatgct
     3241 cccaccagac aagcagaggc ggctggctga cacgccggat gacaacgcca agtatgaggt
     3301 tgtgcactgc gtttttcccc gcacggacta tgacatcacg aaggtggatg ccaaaggtca
     3361 gcggtttgct tctgtctggc tggatgcgga ggctgaagcg gtgcttcgta aaggcggatt
     3421 tgattccatg ccgtatcgca ttgggcggtt tgaccgctcg cccaatgaat tgatggggcg
     3481 ttcgcctgcg attgagcttt tgccggacat caagatgctc aatagcatgc ggcgaacttt
     3541 cgtgatgtcc tgtgagctgc agtcctgtcc agccctgatt gtggaagacg acagtgttat
     3601 cggccagcct tccaccggcc caggcgatat tatcgtcaag cggcatgggg ctgacgaccc
     3661 caggcccctg cagaccggag ccaaccccca gctaaccgcc gaagctattg cagtggagcg
     3721 ggaagggatt atgcagggct tttttatgga catgtttgat gtgctggagc ggtaccgcaa
     3781 tatgaccgcc tacgaggcct cccgccgcat agaggacaaa caggtattaa ttgccccgat
     3841 gatttcaggc ctgcagaagg aaatgctgga tccgttaatt ttgaggtcat tggatttaat
     3901 cagcagcaag aagcggctgg ccgagccggt atcgtctgaa gacggcctgc agattgttta
     3961 tcagggtcgt ctgtctttgg ccatgagcaa cctccaggcc agtgctattg agctgactct
     4021 ggccaaatgg tcgccgtatc aggctatgta tcccgttctg gacaatgtca atctggacaa
     4081 gtcttttgta atgtctgcac ttaacgccgg cgtgccggcg gactgcctgc ggagtcagcg
     4141 tgagcttgag cagattcgcc agcaggccca gcaggtccag caggcacagc agacggttga
     4201 gctggccgag aagggttcaa aggctattcg caacctctcg ggtacttctt tggaggggct
     4261 ggttctatga acgagataga ggttcgcaat cgcaaagcca agcaggcgat ggatgaatta
     4321 tctcgggcgt acaagacagt tttttcaggc caagccggcc agcgtgtgct ggctgacatc
     4381 agccggtttt gcggtgctga tgccgtcagt gcttcagagt cccagccgaa cgctttgacg
     4441 acttttttta gagaaggaaa acgcagtgtt tttctgcgga ttatatggca attaaacagg
     4501 aaggaatcga atgactgaat taaacgcgca aacaagccaa gccgctgccc aaacggcaag
     4561 tccggcacag ggatgggtcg caggagatgg gacatttgcc aaccatcaaa tgatgcctga
     4621 agatgtccgc aatgtcgtgc tgtcgaaaaa atggtccaat gtagcagata ttgttaagtc
     4681 ttatcgagaa ctggaatctt ttattggcgg acacaagagc aggctgaata ttccagacaa
     4741 tctggacgat tccgccttaa atgagattta ctccaagctt ggccggcccg atacgccgga
     4801 caagtaccag ctgcaggcca aagtgcctga agggatgcag atggataatc agctggtgga
     4861 gcgctttaag aagtttgcgc acgccaaaaa cctcaccacc acccagttca acgacctcgt
     4921 ccaattccag ctggatgctg ccgcagaggc cgccaaggct gatgagcagg cacgccttaa
     4981 gcagattgag gaggccgcta ttgcgctgaa gcaggaatgg aaggacgctt atgcatccaa
     5041 tttcagcact gccaagcatg cggctgaaaa actgggcatt atggatgagc ttgaaaaact
     5101 cggcttggcc gacaatccgg cggtgattcg catgctgtat aaattgggca caaaactttc
     5161 cgaggacacg ttgggcgtgc gtacaaacgc accggcgatg tccagagaag aagaattgaa
     5221 aaaactgctg gcatctccgg aattcaacga caagatgcat cctggaaacc aggcggcatt
     5281 ccggcgattc ctggagttgc acggcttaac tggacaagct taatcgcccc agaccctatc
     5341 attgctgcgg ccccgttgtt gggacaacca aagcagccat cggattaaca acataaaaaa
     5401 cgaaggagtt ttatcatgtc tgaccccaac tacatggcac ttttctacaa tgcttacaat
     5461 gcagcccgtc tgcatgtacc ccaggaacgc aaaaacccct ttgaaggcac ggtgaccacc
     5521 gatgttctgc gcggcgaaca gcagtcgtac gacgatttcg gcatctcgaa cctgaagcag
     5581 aagaccacaa ggtttgcgac agtcgaaacc accgacaatg atatgcgcag gcgatggctg
     5641 tttgccaatt ggtactatga tgccaaactg gtggacagac aggacaatat cgccctgctg
     5701 tctgacccca caggtgactt cattaaatct cttgcttacg gcgtcgagcg gctgaagcgg
     5761 ggtgtaatcc ttacggcttt tgaagcatcc gtcagcggcg gcaaaaatcc aggcgggacc
     5821 tcttacagct tcagcaacac tgccatcggc agcgggggtg cggtaatcgt gcatgatacc
     5881 acctatgccg gtgccgcagg cgggacatcc aaaggcttgt caaccgataa gctgattttg
     5941 attcgtgaga ccttcagccg ctggggcgtg cctgacggtg tgcccattta tctggcctgc
     6001 accttccgcc agaaatccga tctcctgcgt gaacagcagt ccaagggcca gaacacttcg
     6061 gaagttgaag ccttggttga cggccgcatt aaccggtaca tgggaatcaa ctttgttgaa
     6121 accaacggga ttaccctgac atccaacggc gacctcgacg gtgatacttc tgtgtttaag
     6181 tgctatgcct atatacccga gggcattgtt ttagcgacat ctccggcccc caactttgct
     6241 gttgataagc gtgctgaatt ggtcggcgat acttggcaga tacgggctga ctttgcggca
     6301 gcagcgattc gccggaacga aaatatggtc attaaggttg agtgtgccaa tactgcttcc
     6361 taaccgaagc taaaggagga ttcttaaatg gcaacttatt atggcgtaaa ttcgacacaa
     6421 gcaaaatcca gcagtatgtc gggccaaatc agcaagcttt ggcacaatcc ccttatgatg
     6481 gtatacgata agtatgtagt acccaccggc ggggttgctg cgggatcgga cattgtgatg
     6541 ggcacaatcc ccaagggcgc tcgggtggtg gggtttatct tccagggcga cgacaacggc
     6601 tcgaccactg ccaccggtac tatctatctg gggacaacgg cagccacggc gtctgcggcg
     6661 attacagacc ttgccagtgg gcctgtagca ctgttcattc cagctctgga taccttctcg
     6721 cagaccccct tgacatccag cacggccgtg tctatcaaga cggccacagg ggctttggca
     6781 gccgacgtgg tgctgacgct gacaacgctg tatgtaatgg attaacctaa caaccaggcg
     6841 gcaaagcggc acggcggctt ttgacctgcc gtgccgcatg ctgccgcatg gagaagtcta
     6901 tgtccttgac cgatgctaaa attgctgtct gcaaccgcgc actgggatat atcggtgagc
     6961 actggattga tgccaacaac accagcagca agccctacga gatgtgcgat aggtattacg
     7021 acgatgccgt aaaagaggtg ctggttgagc atgcatggaa tgaagccaag acacgcagca
     7081 ttgttgtgca ggaagccgat gaacccctat tcggctatac ctatgcattc gccctgccta
     7141 ccgactgtct gcgtgtcctg cgaatcggca acggctcaat ggacacgcag gattgggagg
     7201 ttgaaggcgg caaaatcctt acgctgtctg cacagcggcc attggattat gttgtcggcc
     7261 agacttatca ggctgggcag tacatcactt attcggacta cacctacagc gtgcacacca
     7321 gttttacagc atcaagctgg gaaacagact ccgcagagta ctgcacatca accggcggag
     7381 attatgcggt actgcagata gaatacatca aaaacctcac cgacacttcg atgtggtcgc
     7441 caaaactgct ggatgccgtt tgtcagcggc tggctatcaa ggtagccgtc ggtctttcaa
     7501 acaatccaag ggccagatac gacctccttg aagagtttga gaaaattacc atgcgaaagg
     7561 cccgttcgat tgatgcacag cagggaaggg ttaagtcgtt tttgaagagc gcttggatac
     7621 gggcgagggg attgtaatgc agaccatcaa aacgacattt aacgccggaa aactttcggc
     7681 ataccttgac ggccgtgtgg acatcagcaa atactacaac ggatgttccc agttggttaa
     7741 tgctatccca ctgtccacag gcggcattac caagcggcca gggacacgct ttatcgccaa
     7801 agctaaaggc ccttgccggc tgattccttt tgaattctct gcggacgata cgctggtgct
     7861 ggaattttca gaccttgctg ttcggttcta caagaattgc gacagggtta tgctgcccag
     7921 cacggcgatt agttctatta cactgctgcc tgatacagag gttgccgtcg acactacaga
     7981 agcacacggg cttcagaccg gcgatgttgt gcggtttgcg gatgtgctgg gcacagatga
     8041 gctgaattac gccggcaatt ttgccactga atggacaatt acaaaaacca gcagcacggc
     8101 gtttacgctg gatgggacga atgggagcaa tttttcggaa tacaaaggcg gcggaactgt
     8161 ggctgggatt tattccattg cttcgccata cacccttgat gatgtttggc agctgcatta
     8221 cacacagtcc ggtgatgtgc tgtatttaac ccacccagac tatccgccgt atacgctttc
     8281 ccgatacggc gatgccagct gggttattca ggaagccaat ttggtcggcg ggcctttttt
     8341 gggcgaaaac acaacagaca catacctgct gggctttacc cgcacaggcg gaatagcccg
     8401 ttgtggacat tactttcccg caggggcaat cggcacgctg actgccagcg gcacaggtaa
     8461 tgcgccgttt ctgccgcagc atgtggggtc gctgtggatg ctgaagcaca caaggccaga
     8521 caacaccatg tccacaccag acaacaccac caatgctgtg ccgacgggca cgggcataaa
     8581 gattaagggg tctttttcgt ttgatgtgac cgtcaactcc aacaccttca aaatctggcg
     8641 gcgtcaaggc aatggtgctt ggcaggaata ccgcacattc ttggccaaca ctgcttacac
     8701 atcagatgaa ttggacgatg atgtgtatta tacattcacc agttcatcgt ccagcggttc
     8761 agccgtcctg acagcaaaag accaaaccaa tgtcggcatt gtgaagatta caggctatat
     8821 ctcgcccaca agcgtctcgg ctacagtcat tcagcctgta ttgagcaata attctgatga
     8881 ctctgcggtt actacatcaa tgtgggcaga aggagcgttt aatgcatatc gggggtaccc
     8941 cagaacactg gtaatccacg aaaaccgttt atggtttgcg tccaccagca ataatcccca
     9001 aaccctttgg ggctcctcct catccaggtt cctgaatttc actgctggcg acaccgacag
     9061 cgatgctgtg actgttacca ttgaagactc cgatgtatcc aatattcagt ggatggcttc
     9121 ggatgaatac ttggtggttg gaacagccaa caaagaatac aaaatatctg ccagcaacat
     9181 tgacgaccca atcacgccgg atgatgtgcg atgccgtgtg cagtctgcat tgggcagcaa
     9241 ttcgatgcag cccgtccatc tcaacaacac cctgttttat tttcaagggg gcggccgcac
     9301 actccgttca atgcggctga tggatgctac cctgcggtat gagtccgaca atgcaactct
     9361 gctggtggat gatatttttg attctttgcc tgtgcaaatc gctgtccagc gggtgccgta
     9421 tgctattttg tggattgtac ggcaggacgg cgctttggtg tcttttacct atgagccgaa
     9481 ggaagaggtt tccgcatgga gcgaacatgt cacgggcggc ttactgctta cgccgtatca
     9541 agggcagttc agtagtgtgt gtgtgattcg aggaatgccg gaggatgaag tgtatgtgtg
     9601 cgtgcggcgc atcatcggcg gcatagagca gtattacatc gagcagtttg ctgctcgtta
     9661 tacaggccgt caggatacgc agtcttttct ggactgctca acggtgttta cagccaacag
     9721 tcagccggtc a
//
LOCUS       DAOTBO010000102         9606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_6074_length_9606_cov_4.550728, whole genome shotgun sequence.
ACCESSION   DAOTBO010000102 DAOTBO010000000
VERSION     DAOTBO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6074_length_9606_cov_4.550728"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLE88_10485"
     CDS             complement(<1..365)
                     /locus_tag="PLE88_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOM76932.1"
                     /translation="MRRQSFFLLAGVSLLMGLGGCNNDILDPTQLGRFEPTPTVNVIL
                     DTLGVADEPAPTYEGAEEPRPEDLIAYESDYVFGPGDIVRISIFELYQEGQTYVNDYI
                     VTETGRLSIPDVGLVTAAGL"
     gene            complement(634..1359)
                     /locus_tag="PLE88_10490"
     CDS             complement(634..1359)
                     /locus_tag="PLE88_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76933.1"
                     /translation="MIRSTISSAVCLCILFLGCSRQSAPISQDSTEIRTQISASCIAR
                     YYKTNGAVYITSQQHSFDPLTGYFQMKALEPAGQLQFTLNKNQYSEIKPKTAVLSDSP
                     VSFCNAQLAAALFYSFCAGGDLLDTASLSSGEPIKLEGQWYQPLQTNWPNPPVVITLL
                     RSLDTNRIELVGLDDADSGLSWLLRSYNLRYNKDLDKRIPRTIDVFDTRSGIASKQLM
                     IQFDYKTVQSAQLLAAGKIQQAE"
     gene            complement(1469..5032)
                     /gene="nifJ"
                     /locus_tag="PLE88_10495"
     CDS             complement(1469..5032)
                     /gene="nifJ"
                     /locus_tag="PLE88_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOM76934.1"
                     /translation="MKRKMVTIDGNAAAAHVAHATNEVIAIYPITPSSPMGEISDAKS
                     AAGQPNIWGTVPSVTEMQSEGGAAGAVHGALLSGALTTTFTASQGLLLMIPNMYKIAG
                     ELLPTVFHVSARSLACQALSIFGDHSDVMACRQTGYAMLASNNVQEVMDMALIAQQAT
                     LASRVPFLHFFDGFRTSHEVQKVEELTYDDMRAVIDETLVAAHKARALSPDRPMMCGT
                     AQNPDVYFQGRETVNRFYLAAPQIVQETMDKLAGVVGRQYHLFDYVGAADAEKVIVIM
                     CSGADTVHETVDYLVSKGEKVGLLKVRLFRPFSVEHFIKALPKTVSKIAVLDRTKEPG
                     SIGEPLYLDVRTAIGEAMADKTAPFAAYPICVGGRYGLGSKEFTPAMVKAVFDNLDRE
                     EPKNHFTVGINDDVCKTSLAVDETFDIPSKSYTAMFYGLGSDGTVGANKNSIKIIGEE
                     TNNYAQGYFVYDSKKAGAMTVSHLRFGPQEIRSPYLVKKADFLACHNPSFLEKYDMLS
                     HIKQGGTFLLTSLHGPDTVWDTLPLEVQQQIIKKKLKFYVINAIQIAEQLGLGSRINV
                     IMQTAFFKISGVIDVDTAVNAIKVAIKKTYGKKGDKVVAMNNAAVDAALSKIYQVKVP
                     AQATSQTHMARMVPDDAPDFVKEVTAEIMAGRGDQLPVSKMPCDGKFPTATTKYEKRN
                     IAVHIPVWEPQLCIQCGQCSLVCPHAAIRIKAYDPKELEKAPPAFKSIDAKGKEFAGM
                     KFTVQVAPEDCTGCSACVVNCPGRDKTDPQRKAINMALQEPLRMQERTNFAFFLSIPD
                     TDPSKFKAASIKGSQLIPAMFEFSGACAGCGETANVKLITQLFGDRAYIANATGCSSI
                     YGGNLPTTPYAKRADGRGPVWSNSLFEDNAEFGLGMRLAINKFKAFALELVAKTAEAG
                     CIEKGLAEEIRTADQTSPAGIEAQRGRVEKLKDACRKSNCADCRQLLMVADYLVEKSV
                     WIVGGDGWAYDIGYGGLDHVLASGENVNVLVLDTEVYSNTGGQMSKSTPRGAVAQFAA
                     GGKRMPKKNMGLMAMSYGGIYVASVAIGANPNQAVKAFAEAESYNGPSLILSYSHCIA
                     HGIDMATGYETQKKAVASGHWPLYRYDPRLAAQGKNPLQLDSKAPTIDFAEFANGQTR
                     FQSLQKTNPQAAAELMELAKKDAAQKYALIEKLAKLECDCK"
     gene            complement(5345..6274)
                     /locus_tag="PLE88_10500"
     CDS             complement(5345..6274)
                     /locus_tag="PLE88_10500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOM76935.1"
                     /translation="MKIPRRTFLGSSILGMGGFLLGRRGFAQEAPAPAGFDPYEKVAL
                     GKTGLKVSRVGLGTGMRGGNQQSNQTRLGKEKFSELIAGCFERGINWFDMADLYGSHP
                     YVLPALNGVERDKVVLVSKIWLGRAGVAIPQEERLDVGKTIDRFLKEIGTDYLDLVLL
                     HCMTDKNWPSRYETQMTVLDQLKKKGVVRAHGVSCHSLDALKTAAAEPWVDSVHARIN
                     PYGTSMDGEPDVVVPVLEQIHAAGKGVVGMKLIGEGRFRDSDLKRNYSVEFVLNLGTV
                     DTMVVGFENLEEVDDFARRVRMAIRRPVRDTVA"
     gene            complement(6365..8068)
                     /locus_tag="PLE88_10505"
     CDS             complement(6365..8068)
                     /locus_tag="PLE88_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76936.1"
                     /translation="MKTRYAAAAVVWIAAAMLPEGSRCMAQPEKPVAIRVDLGRTKGP
                     MRPVWAWFGYDEPNYTYMKDGRKLLSELSALSPVPVYVRAHNLMCTGDGTAALKWGST
                     NMYTEDAQGNPVYDWTLIDRIFDTYIQRGMKPLVEMGFMPKALSIQPEPYQHQWRPGL
                     PYREIFTGWAYPPKDYGKWAELIYQWVSHCLQRYGRQEVASWYWELWNEPDSGYWQGT
                     VEDYCRLYDYSADAVKRALPEGRVGGPHVTGPSGQGAARFLRAFLTHCFEGTNAATGR
                     RGAPLDFIAFHAKGAPRVVDGHVRMNMGRQLRDISSGFEIVASFEPFRHLPVIIGESD
                     PEGCAACSMQDYPENAYRNGTLYSSYTAASFARKYELADLFGVNFLGAVSWSFEFEDQ
                     PWFNGYRDLATNGVDKPVLNVFRMFGLMAGQRVEVSGDLAYDVLTVRDSSIRGPRPDI
                     SALAAADERSASVMVWNYHDDDVDAADATVEVTVVSVPAVQVLLHHYRVDRRFSNSYE
                     VWKKMGSPQNPTPQQTAELERAGQLELLAAPVWLKTQDGRLTLPMTLPRQGVSLLKLT
                     W"
     gene            complement(8119..8886)
                     /locus_tag="PLE88_10510"
     CDS             complement(8119..8886)
                     /locus_tag="PLE88_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM76937.1"
                     /translation="MKRMVVFFLVGGLLAGSAGTAAGAEPPKPAEPNGFEKDVFQTDI
                     GPLTITFIGHGTLMADCAGKVIHIDPWTRLADYSKLPKADLILITHEHADHLDAKAVQ
                     QLRRPDTAVILTKACAEKIAGGTILANGQTTQAAGFKIEAVAAYNIQHTRPDGVPYHP
                     KGQGNGYIITLGKTRLYVAGDTENTPEMKALQSIDIAFLPVNLPYTMTPQMAADAAKA
                     FRPKVLYPYHYGQTDLSTLQALLAEDKDIEVRIRRLP"
     gene            complement(8915..9457)
                     /gene="cobO"
                     /locus_tag="PLE88_10515"
     CDS             complement(8915..9457)
                     /gene="cobO"
                     /locus_tag="PLE88_10515"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOM76938.1"
                     /translation="MFEKGLVQIYTGDGKGKTTAALGLAVRAAGHGAKVLFYQFLKPA
                     DLDLGERSLIRSQCQAITMRWLDEPWDMFRSMHDQRQIERIRTAIHKALQELETAAHE
                     KYYDLIVLDEIVFCLNAGFARMEDIRRLLAHRDRQVEIVLTGRGASGELMELADLVTE
                     MRPVKHPYETGVKARKGIEY"
BASE COUNT         2081 a         2941 c         2464 g         2120 t
ORIGIN      
        1 agaccagccg ccgtaacaag gcccacatcc ggaatcgaaa ggcggcccgt ctcggtgact
       61 atataatcat ttacatacgt ctgcccttcc tgatacagct caaaaatgct gatgcgcaca
      121 atatcaccgg gtccgaaaac atagtccgat tcatatgcga tgaggtcttc cggccggggt
      181 tcttcggcgc cttcatacgt cggggcaggt tcatcagcca cacccagcgt atccaggatc
      241 acattcaccg tcggagtggg ttcaaatcgg cctaactgcg tcggatccaa tatatcatta
      301 ttgcatcctc caagacccat aagcaatgac acacccgcaa gcagaaaaaa actttgtctt
      361 ctcatggcct aaaatccatt ctaaaaattg ccctttttcg tctgccttag gcagacaccg
      421 cattcttaaa actttcagaa agaaaagaga aaggttctta cttcccaatc ggtcaaatcc
      481 gaccgtccct taacctttaa tttcggcttt aacatctgaa aaacaacaaa attatcgtaa
      541 acaggacatt acggaaaaac tccatcgtct ttattaaatc gaggattttt ccctgtagaa
      601 taaaacattc tacattataa ttatcgcaaa tgttcactcg gcttgctgaa ttttacctgc
      661 tgcaagcagt tgggccgact gaacagtttt ataatcaaat tgaatcatca gctgcttgga
      721 tgcgattccg ctgcgcgtat caaacacgtc aatagttctc gggattctct tgtctaaatc
      781 cttgttataa cggaggttat aacttctcaa cagccacgac aagccgctgt cggcatcgtc
      841 tagcccaacc agctcgatgc ggttggtatc cagcgaccgc agcagcgtaa tgacaaccgg
      901 cggattcggc caatttgtct gaagcggctg gtaccattgc ccttccagct tgataggttc
      961 tccagaagaa agcgatgcgg tatccagcaa gtcgccgccg gcacaaaaac tatagaaaag
     1021 ggctgcagcc agctgggcat tgcagaaaga aacaggactg tccgataaaa ccgcagtttt
     1081 cggctttatc tcactgtatt gattcttatt cagcgtaaat tgaagctgac cagccggttc
     1141 gagagccttc atttgaaaat aaccggtaag gggatcgaag ctgtgctgct ggctggttat
     1201 atagaccgcc ccattggtct tgtaataacg ggcaatacag ctggctgata tttgtgtcct
     1261 tatttcggtg gaatcttggg aaatcggtgc cgattgccgg ctgcatccga gaaagagtat
     1321 gcataaacaa actgcactgc tgattgtcga gcgtatcata cctgttttcc tcttttcagg
     1381 acaactcatt ttatctgccg ccgcagggcg cccgctatac attataaagc ccagccccgc
     1441 agaagcggag ccgggctgga aaaagctgct atttgcagtc gcattccaat ttggccagtt
     1501 tttcaatcaa ggcatacttt tgagccgcat ctttcttggc cagctccatc aattccgcag
     1561 ctgcctgcgg attggtcttc tggagcgact ggaagcgggt ctgcccattg gcaaattccg
     1621 caaagtcaat cgtcggggct ttggaatcca gctggagcgg atttttgccc tgagccgcca
     1681 gccgcgggtc gtagcggtaa agcggccagt gcccgctggc gacggccttc ttctgcgtct
     1741 catagccggt ggccatatca atgccgtgag cgatgcaatg gctgtacgac agaatcagcg
     1801 acggcccatt gtagctttcc gcctcggcaa atgccttgac ggcctgattc ggattggcgc
     1861 cgatggcaac ggaggccaca taaataccgc cgtaactcat cgccattaag cccatattct
     1921 tcttgggcat ccgcttgccg ccggcggcaa actgggcaac tgccccgcgc ggcgttgact
     1981 tggacatctg accgccggta ttggaataca cttcggtgtc cagcaccagc acattcacat
     2041 tttcgccgct ggccagaaca tggtccagtc cgccgtaacc gatatcgtag gcccagccgt
     2101 cgccgccgac aatccagacg ctcttttcaa ccagataatc ggccaccatc agaagctgcc
     2161 ggcaatcggc acagttgctc ttgcggcagg cgtccttgag cttttcgacc cggccgcgct
     2221 gggcttcaat ccctgccggc gaggtctggt cggccgtgcg gatttcttcg gccagtccct
     2281 tctcgatgca gccggcctcg gccgtcttgg caaccagttc cagagcaaac gccttgaact
     2341 tgttgatggc cagacgcatt ccaagaccga attcggcgtt atcctcaaac aagctgttgc
     2401 tccagacagg acccctgccg tcggcccgtt tggcataggg cgtggtgggc aggttgccgc
     2461 cgtaaatcga cgaacagccg gtggcattgg caatatatgc acggtccccg aacagctgcg
     2521 taatcagctt gacattggcg gtttcgccgc agccggcaca ggcgccgctg aactcaaaca
     2581 tcgccggaat gagctggctg cccttgatgc tggcggcctt gaacttggac ggatccgtat
     2641 cgggaatcga caggaagaac gcaaaattgg tgcgttcctg catccgcagc ggctcctgca
     2701 gcgccatatt gatggcctta cgctgcggat cggttttgtc cctgcccggg cagttgacca
     2761 cgcaggccga acagccggtg cagtcttccg gagccacctg aacggtaaac ttcattcccg
     2821 caaattcctt gcctttggca tcgatgctct tgaaggccgg cggggccttt tccagctctt
     2881 tcgggtcgta ggccttgatg cgaatggccg catgggggca caccagcgag cactgcccgc
     2941 actggatgca caactgcggc tcccagacgg gaatatgcac ggcaatattg cgcttctcgt
     3001 acttggtggt tgccgtgggg aacttgccgt cgcagggcat cttgctgacc ggcagctggt
     3061 cgccgcggcc ggccataatt tcggccgtaa cctccttgac aaagtcaggc gcatcatccg
     3121 gaaccatccg cgccatatgg gtttggctgg tggcctgtgc cggaaccttg acctgataaa
     3181 tcttgctcag cgccgcatcg acggccgcat tgttcatcgc cacaacctta tcgcctttct
     3241 tgccgtaggt cttcttgatg gccaccttaa tcgcattgac ggcggtatcc acatcaatca
     3301 cgccgctgat tttgaagaag gccgtctgca taatcacatt gatgcgcgag cccagcccca
     3361 gctgttcggc aatctggatg gcattgatga catagaattt gagcttcttt ttgataatct
     3421 gctgctggac ttccagcggc agggtatccc agacggtatc ggggccgtgc agactggtca
     3481 gcaggaatgt gccgccctgc ttgatatggc tgagcatatc atacttctcg aggaagctgg
     3541 ggttgtggca ggccaaaaag tcagccttct tgaccagata cgggctgcgg atttcctgcg
     3601 gaccaaaccg cagatgcgat acggtcatgg caccggcttt cttggagtcg tacacgaagt
     3661 agccctgtgc atagttgttg gtctcttcgc cgataatctt gatgctgttc ttgttggccc
     3721 cgacggtccc atccgaaccc agaccataga acatggccgt ataagacttg ctggggatat
     3781 caaatgtttc atccacggcc agactagtct tgcagacatc gtcgttgatg ccgacggtga
     3841 agtgattctt cggctcttcc cggtcgaggt tgtcaaagac ggccttgacc atcgccggcg
     3901 taaactcttt ggaccccaga ccgtaccggc cgccgacaca aatcggataa gccgcaaacg
     3961 gggcggtctt gtccgccatc gcctcgccga tcgccgtccg cacatccagg tacagcggct
     4021 cgccgatgga gcccggctct ttggtgcggt ccagaaccgc tatcttggag actgtcttgg
     4081 gcagcgcctt gataaagtgc tcgacgctga acggacggaa cagacgcacc ttcagcaggc
     4141 cgaccttttc gcccttgctg acaagataat cgaccgtctc gtggacggta tcagcccccg
     4201 aacacataat cacaatcacc ttctcggcgt cggcggcgcc gacataatca aacaggtgat
     4261 actggcggcc gaccacgccg gccagcttgt ccatcgtctc ctgaacaatc tgcggggcgg
     4321 ccagataaaa ccgattgacg gtttcgcgcc cttggaaata cacatccgga ttctgtgcgg
     4381 ttccgcacat catcggacgg tcgggactca gcgctcgggc tttatgggca gccaccagcg
     4441 tctcatcaat caccgcccgc atatcatcgt aggtcagctc ttccaccttc tgcacctcgt
     4501 ggctggtgcg gaagccgtcg aagaaatgca ggaacggcac gcgcgacgcc agcgtcgcct
     4561 gctgggcaat cagcgccata tccatcactt cctgcacatt gttggatgcc agcatcgcat
     4621 agccggtctg ccggcaggcc ataacatccg agtggtcgcc gaaaatgctc aaggcctgac
     4681 aggccagcga gcgggccgat acatggaaga ccgtcggcag cagttcgccg gcgattttgt
     4741 acatattggg aatcatcagc agcagcccct gcgaggccgt aaaggtcgta gtcagcgcac
     4801 cgctgagcag cgccccgtgc accgcaccgg cggctccgcc ttcggactgc atttccgtca
     4861 ccgacggcac cgtcccccag atattcggct ggccggcggc acttttggca tcggaaatct
     4921 cccccatcgg ggaactgggc gtaatcggat aaatcgctat cacctcattg gtggcgtgag
     4981 ccacatgggc ggcagcggca tttccatcaa tcgttaccat ttttcgcttc atacatttct
     5041 ccacaaaaaa aatctcttga aaagaaaact aagaatcaaa ccatacgctt gaagttttat
     5101 aaaaaataga ttgtttctta taccacgtcc cggcaaaact ggcaaaccct tttttagccc
     5161 cttttatgca aaatgccgat gcctcattcc cgaacaatgg atactcgcct cccccagatg
     5221 cggccggcag ctgcctgcca gacagaaaat cccctctgag aaacctgcct tttgcctgta
     5281 agtcccttgc gtacaggatg taaaggcagc ggacaccaac gccggcagaa tcccccgccg
     5341 gcattcaagc cacagtatca cggacgggcc gtcgaatggc catcctgacc cggcgggcga
     5401 aatcgtccac ttcctccaga ttttcaaatc cgacgaccat ggtgtccacc gtacccaaat
     5461 taaggacaaa ctcgaccgaa taattgcgct tcaggtcgct gtcccgaaac cgcccttctc
     5521 cgatcagctt cattcccaca acacccttgc cggcggcgtg tatctgttcc agaaccggta
     5581 caaccacatc cggttcgccg tccatcgaag tcccgtacgg attgattcgg gcatgcaccg
     5641 aatcgaccca cggttcggcg gcggctgtct tcaacgcatc cagcgaatga cacgatacgc
     5701 cgtgcgcccg tacaacaccc tttttcttca gctggtccag aaccgtcatc tgcgtttcat
     5761 atcggctcgg ccagttttta tcggtcatac aatgaagcag caccaaatca agataatctg
     5821 tgccgatttc cttcaaaaac cggtcaatcg ttttgccgac atccagccgc tcttcctgcg
     5881 gaatcgccac ccctgcccgg cctaaccata tcttgcttac aaggacaact ttgtcccgtt
     5941 caacaccgtt caaagccggc agcacatacg gatgactgcc gtacaggtcg gccatatcga
     6001 accaattgat cccccgctca aagcagccgg caatcagctc cgaaaacttc tcttttccca
     6061 agcgggtttg gttggactgc tgattgcccc cgcgcatgcc tgttcccaga cccacccgcg
     6121 acaccttcag ccctgtcttg ccgagggcga ccttttcata cgggtcaaaa ccggccggag
     6181 ccggcgcttc ctgtgcaaaa ccacgccgcc ccaataaaaa tccgcccatt cctaaaatgg
     6241 aactgcccag aaatgtcctg cgcggtatct tcataaaaca cccctctata taaaatggtt
     6301 attttgccgg tcattatagg cctatctgca gcgcctgtca aacagccgtt tatttttgcc
     6361 ccgcttacca cgtcagcttc agcagggaga cgccctgccg cggcagtgtc atcggaaggg
     6421 tcagccggcc gtcttgtgtc ttcagccaga ccggcgcagc cagcagttcc agctggccgg
     6481 cacgctccag ctcggctgtc tgctgcggcg tgggattctg gggagaaccc atcttcttcc
     6541 agacctcata ggaattactg aaccggcggt ccacacgata gtggtgcagc agcacctgta
     6601 cagccggaac agagacaacc gttacttcta ccgttgcatc ggcagcgtca acatcgtcat
     6661 cgtggtagtt ccagaccatc actgaggcag accgctcatc ggcggccgcc agcgcgctga
     6721 tatccggccg gggaccgcgg atgctgctgt cccgcaccgt cagcacatcg taggccaaat
     6781 cgccgctgac ctcgacccgc tgtccggcca tcagaccgaa catccgaaag acattcaaaa
     6841 cgggcttgtc aaccccgttg gtggccaaat cgcggtagcc gttgaaccac ggctggtctt
     6901 caaactcaaa cgaccacgaa accgccccga ggaaattcac cccgaacagg tcagccagtt
     6961 cgtatttgcg ggcaaaggaa gcggccgtat agctggaata cagcgtcccg ttgcggtagg
     7021 cattttccgg ataatcctgc atcgagcagg ccgcacagcc ctccgggtcc gattcgccga
     7081 tgatgaccgg caggtgtctg aacggctcaa acgatgcaac aatctcaaag ccgctggaga
     7141 tatcgcgaag ctggcggccc atattcatcc ggacatgccc atcgaccacg cggggcgctc
     7201 ctttggcgtg aaaggcgata aagtccagcg gggctccccg ccggcctgtt gcagcattgg
     7261 tgccttcgaa gcagtgggtc aggaaagccc gcagaaagcg tgccgccccc tgtccggacg
     7321 ggccggttac atgcggaccg cccacacgcc cttccggcag cgcccgcttg accgcatcgg
     7381 ctgaatagtc atacagccgg cagtagtcct caaccgtgcc ctgccagtag ccgctgtccg
     7441 gctcgttcca cagctcccag taccagctgg caacctcctg ccggccgtac cgctggaggc
     7501 agtggctgac ccactgatag atcagctcag cccatttgcc gtaatccttc ggcggatatg
     7561 cccagccggt aaaaatctcc cggtacggca gccccggacg ccactggtgc tgatacggtt
     7621 ccggctgtat cgacagcgcc ttgggcataa agcccatttc caccagcggc ttcattccgc
     7681 gctggatgta ggtatcgaag atccggtcga taagcgtcca gtcataaacc ggattgccct
     7741 gtgcatcttc ggtgtacata ttggtcgaac cccatttgag ggcggccgtt ccgtcgcctg
     7801 tgcacatcag attgtgcgcc cgcacataca ccggcaccgg actcagcgcc gacagctccg
     7861 acagcagctt ccggccgtct ttcatatagg tgtagttcgg ctcgtcgtag ccgaaccatg
     7921 cccacaccgg ccgcatcggc cccttggtcc gtcctaaatc cacacggatc gcaaccggct
     7981 tttccggctg ggccatgcag cgggacccct ccggcagcat cgcggcggct atccatacaa
     8041 cagccgcggc ggcataacgg gtcttcatac cttgctcctt tccatcgggt atctgtataa
     8101 ttgtcttccg ccagtccgct acggcagccg gcgaatccgc acctcaatgt ctttgtcctc
     8161 tgccagaagc gcttggagtg tgctgagatc cgtctggccg tagtgatagg gatacagcac
     8221 cttcgggcgg aaggccttgg ccgcatcggc tgccatctgg ggcgtcatcg tatagggcag
     8281 attcaccggc aggaaggcaa tatcgatgga ctggagggcc ttcatctccg gcgtgttttc
     8341 ggtatcgccg gcgacataca gacgcgtctt gccgagggtt ataatatagc cgttgccctg
     8401 ccccttggga tgatagggca caccgtccgg acgggtgtgc tggatgttgt aggccgccac
     8461 ggcttcaatc ttgaatccgg cagcttgagt cgtctgcccg ttggcaagaa tagtgccgcc
     8521 ggcaatcttt tcggcacagg ccttcgtcag aatcacggcc gtatccggcc ggcgcagctg
     8581 ctggacggct ttggcatcca gatggtcggc atgctcgtgg gtaatcagaa tcaaatcggc
     8641 cttgggcagc ttggaatagt ccgccagacg cgtccacggg tcaatgtgaa tgaccttgcc
     8701 ggcacagtcg gccatcagcg tcccgtgacc gataaaggta atcgtcagcg gaccgatgtc
     8761 ggtttggaag acatcctttt caaagccgtt gggctcggcc ggcttggggg gttctgcacc
     8821 ggcggctgtt ccagcgctgc cggcaagcag tccgcccacc agaaaaaaaa caaccattcg
     8881 cttcatacat cctgcctcgc tttcttgtta accgttaata ttcaatgccc ttgcgggcct
     8941 tcacgcccgt ctcatacgga tgcttgaccg ggcgcatttc cgtcaccaag tcggccagct
     9001 ccatcagttc cccgctggcc ccgcggccgg tcagcacgat ttccacctgc ctgtcgcggt
     9061 gggccaaaag ccggcggata tcctccatcc gggcaaatcc cgcattcaga caaaagacaa
     9121 tctcatccag aacaatcagg tcatagtatt tttcgtgggc ggccgtctcc aattcctgca
     9181 acgccttatg aatggccgtg cgaatccgct ctatctgcct ctggtcgtgc atcgagcgga
     9241 acatatccca cggctcatcc agccagcgca tcgtgatggc ctgacactgg gagcgaatca
     9301 gactccgttc gcctaaatcc agatcggcag gctttagaaa ctgataaaaa agcacctttg
     9361 ccccatgtcc ggcggcacgg acggccagcc ccaaggccgc tgtggttttg cctttgccgt
     9421 cgccggtgta tatctgcaca agtccctttt caaacatagc cgccattcta ccggccgaag
     9481 ggacacaatt caaatgcaat ttaacctgcc cagtaaacac cccccagcgc cggctgtatt
     9541 aacccgacag ctattgtcca gatggtccca attctgcatg cactatcttg ctatcttcaa
     9601 aaatct
//
LOCUS       DAOTBO010000103         9384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_6249_length_9384_cov_6.239897, whole genome shotgun sequence.
ACCESSION   DAOTBO010000103 DAOTBO010000000
VERSION     DAOTBO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6249_length_9384_cov_6.239897"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLE88_10520"
     CDS             complement(<1..682)
                     /locus_tag="PLE88_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM76939.1"
                     /translation="MKKKLCVVMMCLGAILATVARADLVARYTFDDGTAADGSGNSNN
                     GVLLGNVAPTTDAERGAVMNFGGADSFIRVVNSPSLNAVASQITIAMWLNIPSTGTRQ
                     LIEKGGTGGSAWYTAPWGIRMESDGRLRIQWGNVAFLYSVSAIPLNEWTHIALTFDST
                     LTENQVKIYFNGVLDAQATWATIPATNTNDLIIGADIYNYPGTAPRWEYIGLMDDIQI
                     FNTALLETE"
     gene            complement(793..1677)
                     /locus_tag="PLE88_10525"
     CDS             complement(793..1677)
                     /locus_tag="PLE88_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM76940.1"
                     /translation="MKPKGFTLIELLVVIAIIALLLSILLPGLKAAKLQAQLTIDMTN
                     LSGLGKAWFAYSQDNHDELIGGNSPGNASGNAGTWTRDPYYCWVDFPISKTNPNAYAS
                     NFDYQEEIKGIENGLLFNYVGKSDSYHCPADKRYLKPPALNGYTTVNGGYRSYSIAGG
                     ARGGAWDAARRRYAATGSFQFVSITKYSQIKTPGSKFIFVEENDGRGLNAGSWDQDMR
                     LVGQPVTAWVWVDLPAMWHFDRSSFGFADGHAEKYKWVDPGLLQTAGVGSHFVKVDPK
                     YPDDFAFVLRGFPYAGVP"
     gene            complement(1742..3415)
                     /locus_tag="PLE88_10530"
     CDS             complement(1742..3415)
                     /locus_tag="PLE88_10530"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM76941.1"
                     /translation="MDRDKLMSQYFFSSLNRQEQELFAKYLKEDPEFLKQFVTESYVQ
                     RRLYDLFVGQDNQKLLFDSAVLQSPYEMDLWAALLNEEKTAPTIELEVSKNEPVLIEK
                     LQKQKIVRKSSKTTLITAVAALAAVILLIVYVQLNPKEFSDPVATLADMVDVRWADPA
                     MSLKRGDRLFTRQGGIQLKEGILKIQMDNGSNVVVEAPSEFEFITYDQIELRYGRLYA
                     NVPRRAIGFIVSTPNSNVIDLGTQFGVKVNIDGGTEVHVLKGKVSLIAGKQSEEGTSE
                     LVQQGQARRVEKGQEAIQAIAINPGAFVRDIDSQNHYVWRGQPLDLADVIGGGNGWGT
                     GQANTRIDPITGKLAQRMEPDGIQRTTPYILVPDLDFVDGLFIPDGRAGAVQVSSEGD
                     MYEGCPETTGRCFYGPFNGSVLPLKTANEVFDHTVQIGGQTFGGPGRPIILLHSNLGI
                     TFDLRKIRHSLPEGTRISSFSALCGVGEGPASSKRAHDYVNFYILVDGQERFRADDME
                     YQSGLKPIDVNLSDSDRFLTLMTTDGSDRSVHLDWSFFVQPQLTIESVQ"
     gene            complement(3408..3944)
                     /locus_tag="PLE88_10535"
     CDS             complement(3408..3944)
                     /locus_tag="PLE88_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM76942.1"
                     /translation="MNSEKKIEEIAVYWTQAQSIVSAFISSTVPNFEDANDVLQSVAV
                     AVVRKYDTYDRKSPFTAWAIGIARNEVLHYKRKYARDKHIFDDELVNKIAQTYHEKSS
                     ELDDMKKALEKCVSKIEGRWKQILQMRYVREMDAKRIAQCLGVSSNAVFVALHRIRLS
                     LRECIQKTMRAREFGSNG"
     gene            4230..7853
                     /locus_tag="PLE88_10540"
     CDS             4230..7853
                     /locus_tag="PLE88_10540"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM76943.1"
                     /translation="MFLLLSGSAAAIPEYSTRLTQNWEFVKGDIGGIWEALRSDKLSN
                     NWGAWQKVVLPHCFNAYDAVDPDTRYYQGPGWYRTQVKIDNPFAGGRILLHFEGAGQK
                     TEVYIDTEKVCSHIGGYDEFIADITDAVNRYKEKPYFKQDSEDRITKKGLIPIAIRCD
                     NSRDLEMIPSDLSDFNLYGGLYRYVNLVYVPAISLKGLHIKTQRASNGSWQLTVQGEL
                     YNPSSLKENTTAKVQILDPDGTEILQSQTILDSQQNSAVLYQTQLQNPVLWSTTSPKL
                     YTCIVTLISSSGQSAVSEQFGFRWFDFAPKGPFYLNGQRLLLHGTHRHEDHAGLAAAL
                     TEELIVKEFNLMKEMGVNFIRLGHYQQSRIALDMCDRLGILVWEEIPWCRGGLGGDRY
                     KQQARDMLTAMIDQHRNHPSVIIWGLGNENDWPGDFEEFDKEKIRSFMAELNDLAHRL
                     DPERKTAIRRCEFCSDIVDIYSPSIWAGWYGGKFTEYKQSSEREMAKVSHFLHVEWGG
                     DSHAGRHTENPYEGLMDIQAAGKTEESGMDYLMTGGRARASRDGNWSESYICDLVDWH
                     LKEQETMDWLTGTAFWIFKDFSTPLRPDNPIPYMNQKGAVERDLTPKESYYVYQSYWT
                     DKPMVRLYAHNWPVRWGREDEKKLVKVYSNCPEAELFVNGKSEGIKQRNSQDFPAAGL
                     RWLVGFKKGMNHLKVVARKDGTEVSDEIVFRYETRQWGKPARLVLKEADRTGDRATLR
                     AALLDDKGIICLDARNVIRFGITGDGKLIDNLGTSRGSRIVQLYGGQAFIDVQLNQGK
                     SVASASCQGCPTAFLELESIPDSINLRSLTAGQQALEMARLDRERILKLAESYLTIKP
                     ISLRDYPAPADSGAGPGDFYSMGDYWWPNPNTPDGLPYVQRDGQSNPANFSVHRLLVR
                     QLRDAVAALAAGYGITADPRYAQKAVELLNTFFVNEHSRMNPHLLYAQAIPGISKGRG
                     IGIIDTLHLAEVPLAVEALKNSKAMTPEVLAGLKQWFADYTEWMTTHPNGLQEMNAAN
                     NHSVAFLVQLAAFARFTEDKEKLQLARERFKKIILPSQMDLDGSFPAELARTKPYGYS
                     IFQLDNMALLCLLLSTEQDNLWDYTLPDGRGMKKAMSFLVDYLKDKSAWPYPPDIEHF
                     EQWPVRQPCLLLAGYALNRPDYLTLFQTLNPDPADMEVRRNMAVTQPLLWLLRAEDVP
                     LLQ"
     gene            7941..9164
                     /locus_tag="PLE88_10545"
     CDS             7941..9164
                     /locus_tag="PLE88_10545"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM76944.1"
                     /translation="MALKKRLFAVCIFGTVLCSASAADKPNWSSARLWVWDINCLQQI
                     KQKIRQQDPFFTAAFEILQKEGKQALTAGPFSVMNKTLVPPSGDKHDYMSLAPYFWPN
                     PDTPDGLPYIRKDGQRNPQSLDSKRDRRQLGQLCDAVEVLSLCYYYTDDPMYAERAAM
                     LVRTWFLDEATKMNPNLNFGQAVLGSSDGRCYGIIETAGFIGIVDSVGLIQNSPFWTA
                     ADQQKLVQWFTDYLTWLTSHPLGLEESQTKNNHSTWYDAQAAAFALFVGNRPLAQQIL
                     ALASQKHIAAKIEPDGRQPHELTRTKSYGYSVMNLRGTFVLARLGEHVGMDLWNCRTE
                     DERCLQKALDYLIFYADPKNKWPYQQIEPIDASALYPLLKQAHAKIRGFDAQPVLKTL
                     SEQSIHSQRSALLFP"
BASE COUNT         2408 a         2394 c         2178 g         2404 t
ORIGIN      
        1 tctcggtttc cagcaatgcc gtattgaaaa tctgaatatc atccatcaat cctatgtatt
       61 cccatcgggg agctgtaccg gggtagttgt agatatcggc tccaataatc aagtcattcg
      121 tgtttgtggc agggatggta gcccatgtcg cctgtgcatc taaaacaccg ttgaaataga
      181 tcttcacttg attttctgta agggtcgaat caaaggtcag ggcgatatga gtccactcat
      241 tcagcggaat cgcagaaaca gaatacagaa aagccacatt gccccactga atgcgcagac
      301 ggccatcgct ttccattcga atcccccaag gggctgtata ccaagcggaa ccgcctgtac
      361 cgcctttttc aatcagctgc cttgttcctg tactggggat attcagccac atggctatcg
      421 tgatttggct ggcaactgca ttcagactgg ggctgtttac cacccgtata aaactgtctg
      481 cccccccaaa attcattaca gcaccgcgtt ccgcatccgt cgtcggagca acattgccta
      541 acagaacgcc gttattgctg ttgccggaac cgtcggccgc cgtcccgtca tcaaaagtat
      601 aacgggccac caggtccgcc ctggcgaccg tcgccagtat cgcacctaaa cacatcatca
      661 caacacataa tttcttcttc ataatcaaac ctccgcaaaa aagttaatat ctgtaatccg
      721 tctcagcctg ccggcactgt ctgtcgtttt cagacccgca tacgacaggc catgccgtcc
      781 attttttgtc tgttacggta caccggcata cggaaagcca cgaagaacaa aagcgaaatc
      841 atccggatat ttggggtcaa cctttacaaa atgggatccc acgccggctg tctggagcag
      901 ccccggatca acccatttgt atttttcggc gtgtccgtca gcaaaaccaa aactgctgcg
      961 gtcaaaatgc cacatcgccg gcagatccac ccaaacccat gccgttaccg gctgtccaac
     1021 cagacgcata tcctgatccc agcttccggc attcaggccg cgtccgtcat tctcctcgac
     1081 aaaaataaac ttgcttccgg gtgttttaat ctgagagtat ttggtaatag agacgaactg
     1141 aaaactgcct gtggcagcat agcgtcgtct ggcagcatcc catgcgccac ctcgtgcccc
     1201 gccggctatg gaatacgacc ggtagccccc gtttacggtg gtataaccat ttaaggccgg
     1261 aggcttcaaa taccgcttgt cagccggaca atgatacgaa tcggacttgc ctacataatt
     1321 aaacagcagg ccattttcga ttccttttat ttcttcctga taatcaaaat tcgatgcata
     1381 tgcgtttgga ttcgttttgg atatggggaa atccacccag cagtaatacg gatcacgggt
     1441 ccaggtgccg gcatttccgc tggcgttgcc gggagaattg cccccgatga gctcatcatg
     1501 attatcctga ctgtaggcaa accaggcctt gcccaggccg cttaaattgg tcatatcaat
     1561 cgtaagctgg gcctgaagtt tggccgcctt taatccgggc agcaaaatcg acagcagcag
     1621 tgcaatgatg gctatcacta ccaataactc aatcaatgta aatccttttg gtttcatcgc
     1681 tatcccctgt ctacttaaaa tcataatcca ttttctcttg tttgttcacc ggcaagcccg
     1741 cttactgtac ggactcgatt gttaattggg gctgcacaaa gaaactccag tccaaatgca
     1801 ctgatcggtc acttccatcg gtagtcatca gcgtcagaaa gcggtcgctg tcagacagat
     1861 taacatcaat cggtttcagc ccggactgat attccatatc atctgccctg aaccgttcct
     1921 gaccatctac caaaatgtag aaattcacat agtcatgggc tcgttttgaa gaagccggcc
     1981 cttctcctac tccacaaagg gctgaaaagc tgctgatgcg tgtgccttcc ggcagtgaat
     2041 ggcgaatttt ccgcaggtca aatgtaatac ccagattgct gtgcagaagg atgataggcc
     2101 gaccgggacc gccgaaagtc tgcccgccga tttgaacagt gtggtcaaag acctcattgg
     2161 ccgtcttcaa cgggagaacg gagccgttaa acggcccgta aaaacagcgt cctgtggttt
     2221 ccggacatcc ttcatacata tcgccttcgg aactgacctg aacagcacca gcccggccgt
     2281 ccggaataaa caatccatcg acaaaatcaa ggtccggcac cagaatataa ggagttgttc
     2341 gctggatgcc gtccggctcc atccgctgtg ccaacttgcc cgtaatcgga tcaattcggg
     2401 tgttggcctg tcctgtcccc catccgttcc cgccccctat cacatctgcc aaatcgaggg
     2461 gctgtccccg ccagacataa tgattctgcg aatcgatatc ccgcacaaaa gcaccgggat
     2521 tgatggcaat tgcctgaatt gcttcctgtc ctttttctac gcggcgagcc tgcccctgct
     2581 gaacaagctc cgatgtccct tcttcggact gcttgccagc tatcagtgac accttgcctt
     2641 taagaacatg cacttccgtg ccgccgtcaa tattgacttt gacgccgaac tgggttccta
     2701 aatcgatgac attggagttg ggtgtgctga cgatgaaacc gatagcccgt cgcggcacat
     2761 tggcatataa acggccgtag cgcagttcta tctggtcata agtaataaac tcaaattcgc
     2821 ttggggcttc tacaactacg ttgctgccgt tatccatctg aatcttaagg attccttcct
     2881 tcagctgaat tccgccctgt cgggtgaaaa gccggtctcc ccgcttgagg gacatagccg
     2941 gatctgccca ccgcacatcc accatatcag ccaaagtcgc aacaggatca gaaaattcct
     3001 tgggattcag ctggacatat acaatcagca gaataacagc cgccagtgcg gcaactgctg
     3061 taatcaaagt tgtcttgctg cttttgcgaa cgatcttctg tttctgtaat ttttctatca
     3121 gaacaggttc atttttgctt acttcaagct ctatcgtcgg agcagttttt tcttcattga
     3181 gaagagctgc ccataaatcc atctcatagg gcgattgcag aaccgcactg tcaaacagca
     3241 gtttctgatt gtcttggcct acaaacaggt catacagacg ccgctgaaca taagactccg
     3301 ttacaaactg cttaagaaac tcaggatctt ctttgagata tttggcaaat agttcctgtt
     3361 cctgccgatt cagcgagctg aaaaaatact gtgacatcag cttatctcta tccattgctg
     3421 ccaaactccc ttgctctcat tgtcttttgg atgcactccc gaagcgaaag ccgaatacga
     3481 tgaagtgcta caaatacggc gttgctggaa actcccagac attgagcaat ccgtttagca
     3541 tccatttcac ggacgtagcg catctgcaaa atctgtttcc agcggccctc tatcttggaa
     3601 acacattttt ccagagcttt tttcatgtca tccaattcgc tgctcttctc atgatatgtc
     3661 tgagcaattt tattgaccaa ttcgtcatca aaaatgtgct tatcgcgggc atatttacgc
     3721 ttataatgca atacttcatt gcgggcgatt ccaatagccc acgccgtaaa aggggatttt
     3781 ctatcataag tgtcgtattt acggactact gctacagcca ccgattgcag tacatcgtta
     3841 gcatcttcaa aattgggtac cgtcgaagat atgaatgccg acacaataga ttgagcctgt
     3901 gtccaataaa cggcgatttc ctcaattttt ttctcactat tcatagccag attctgcgtt
     3961 tatcattact gtgcatcatt aatcacccat caattctaac atctttcgca gaaaaaattt
     4021 cacagactat attttcttta aaatttttgt ggttacgctt tgtctatttt tatgaaatct
     4081 ctatattcat tgtaagaaac gtttcctatc gggtgatatc tgtttagttt ggataaatct
     4141 tttagaatgg aatttttgtg aagctacttt tcaaagaaag gaatcccatg tcttcctgct
     4201 cggcctgtct tttcttaaca gcagtttact tgttcttatt attatcgggc tctgctgcgg
     4261 caatcccgga atattctact cgattaaccc agaattggga atttgtcaaa ggcgatattg
     4321 gcgggatttg ggaagcactt cgcagcgaca agttaagcaa taactggggc gcttggcaaa
     4381 aagttgtcct gccgcattgt tttaacgctt atgacgccgt tgatcctgat acgcggtatt
     4441 atcaaggtcc cggctggtat cgcacacaag taaagattga caatcctttt gcaggcgggc
     4501 gcatacttct gcattttgaa ggggctggtc agaaaacaga agtatatatc gatacagaaa
     4561 aagtctgttc tcacatcggc ggatatgacg aatttattgc agacattaca gatgcagtca
     4621 atcggtataa agaaaaacca tattttaagc aagattctga agatcgcatt accaaaaaag
     4681 gacttattcc cattgccatt cgctgtgata actcccgcga ccttgaaatg attccatctg
     4741 atctaagtga ttttaatctc tacggcggtc tgtaccggta tgtcaacctt gtctatgttc
     4801 cggcaatctc tctgaaaggg cttcatatta agacccaacg ggcaagcaat ggaagctggc
     4861 agttaactgt tcagggagag ctttacaatc cctcatccct taaggaaaac acaacagcaa
     4921 aagtccaaat tctggatccg gatggtaccg agattcttca atctcaaact atccttgatt
     4981 cccagcaaaa cagcgccgtt ctttatcaga ctcaattgca aaatccggtt ctttggtcaa
     5041 caacctcccc caaattatat acatgcatcg taacccttat aagcagcagc ggccaatctg
     5101 cggtcagcga acaattcggc tttcgctggt ttgattttgc ccccaaaggg cctttctacc
     5161 ttaatggaca gcgcttattg cttcacggca cccaccgtca tgaagatcac gcaggtctgg
     5221 ctgctgcatt gacagaagaa ctgattgtca aagagtttaa tctgatgaag gaaatgggag
     5281 tcaatttcat ccgtttgggg cattatcagc aatcccgcat cgctttggat atgtgcgata
     5341 gattgggaat cctcgtttgg gaagaaatcc cctggtgccg gggcggccta ggcggggatc
     5401 gttataaaca gcaagcccgc gacatgctga cggcaatgat tgaccagcac cggaatcatc
     5461 catcggttat tatctggggt ttgggcaatg agaacgactg gccgggtgat ttcgaggaat
     5521 ttgacaagga aaaaatccgc tcgtttatgg ccgaattgaa tgaccttgcg caccgtcttg
     5581 atccagagcg taaaactgct atccgtcgct gtgaattctg cagcgacatt gtcgatattt
     5641 attcgccgtc aatttgggca ggctggtatg gcgggaaatt caccgaatat aagcaatcct
     5701 ccgaacggga aatggccaaa gtcagccatt ttctgcatgt tgaatggggc ggcgacagcc
     5761 atgccgggcg ccatacagaa aatccctatg aagggctgat ggatatccaa gcggccggca
     5821 aaaccgaaga aagcggcatg gattacctga tgaccggcgg tcgggcacgt gcttcacggg
     5881 acggcaactg gtcagaaagc tatatctgcg atttagtgga ttggcatttg aaggagcagg
     5941 agacaatgga ttggctaacg ggcacggcat tctggatatt taaagacttc tccacccccc
     6001 ttcgcccgga taatccgata ccttacatga accaaaaagg cgccgttgaa cgggatttga
     6061 ctcccaaaga atcatattac gtgtatcagt cttattggac tgacaagcca atggtgcgcc
     6121 tctatgccca taactggccg gtacggtggg gcagggaaga cgaaaaaaaa ctcgtcaagg
     6181 tgtattccaa ctgcccggaa gctgaactgt ttgtcaacgg caaatcggaa gggattaagc
     6241 aaagaaacag ccaagatttt cctgccgccg gactgcgctg gctggttggc ttcaaaaaag
     6301 gaatgaatca cctcaaagtc gtcgcacgca aagacggtac tgaggtttcg gatgaaattg
     6361 tattccgcta cgagactcga cagtggggca aacccgcccg tttggtatta aaggaagcag
     6421 accgaacagg cgaccgggca acactgcgtg ccgcactgct ggatgacaaa gggattattt
     6481 gtcttgatgc aaggaatgtt atccgtttcg gcataacggg agacggaaaa ctgatcgaca
     6541 atctcggcac atcgcgcggt tcccggattg tccagttgta cgggggacag gccttcattg
     6601 atgtacaatt aaatcaagga aaatctgtgg caagtgcatc ctgccaagga tgtccaacag
     6661 catttctgga acttgaatcg atccccgaca gcatcaatct acggtccttg actgccggcc
     6721 agcaggcctt ggagatggcc cgtctggatc gggagcggat tttaaaactg gccgaatcct
     6781 acttgaccat caagccgatt tcgcttaggg attatccggc ccctgctgat tccggtgccg
     6841 gcccgggaga tttttattct atgggagatt attggtggcc caacccgaat accccggacg
     6901 gacttcccta cgtgcagcgg gacggtcaat ccaaccctgc caatttctct gtacatcgcc
     6961 tgctggtacg gcagttgcgg gacgccgtgg cggctcttgc cgccggatat ggaatcactg
     7021 ccgatccgcg ctatgcccaa aaggcggttg aactgctgaa cacttttttt gttaacgaac
     7081 attcgcggat gaatccgcat cttctttatg cacaggccat tcccggcatc agcaagggac
     7141 gcggcatcgg catcatcgat acgctgcatt tggctgaagt tccattggct gttgaagcat
     7201 taaaaaattc aaaagctatg acgcccgaag ttttggcagg actgaagcag tggtttgccg
     7261 attataccga atggatgacc actcacccca acggacttca ggaaatgaat gcggccaata
     7321 atcacagcgt tgcatttctg gttcaattag ccgcttttgc acgttttact gaagacaagg
     7381 aaaaactgca gctggcccgc gaacggttta aaaagatcat cttgccatcc cagatggatt
     7441 tagacggaag tttcccggct gaactggcac gcaccaaacc ctacggctat tctatttttc
     7501 aactggacaa catggcgctt ctgtgcctgc tgctttcaac agaacaagat aatctctggg
     7561 attatacatt gccggacggc cgcggcatga aaaaggccat gtcatttttg gttgattacc
     7621 tcaaagacaa atccgcatgg ccgtatccgc cggatatcga gcattttgag caatggcctg
     7681 tccgccagcc ttgtctgctg ctggctggtt atgccttaaa ccggcctgac tatctcacgc
     7741 tctttcaaac gcttaatcct gaccctgcag atatggaggt tcggcgaaac atggcggtaa
     7801 cgcagccgct tttgtggctg ttgcgtgcag aagatgtgcc gcttcttcag taaaaaacgg
     7861 aatatatccg taaatcgggc aatatcggtt ctgacaggag cgtttctatg gtaaacctgc
     7921 tataaggata aggcccgatt atggcattga aaaaaagatt attcgctgtt tgcatttttg
     7981 gcactgtttt atgttcagca tctgccgccg acaagccgaa ttggtcatcc gcccggcttt
     8041 gggtatggga tataaattgc ctgcagcaga ttaagcagaa gatacggcag caagacccct
     8101 tttttacggc ggcattcgaa atccttcaga aagaaggcaa acaggcatta acggccggac
     8161 ccttcagcgt gatgaacaaa acattggttc ctcccagcgg agataagcat gattatatga
     8221 gtctggcccc ctatttctgg cctaatccgg atacgcccga cggtctgcct tatatccgca
     8281 aagacgggca gcgcaatccg caatcgctgg attccaaaag agaccgccgg cagctcggcc
     8341 agctgtgcga tgctgttgaa gtgctttcgc tgtgctacta ttacaccgat gatccgatgt
     8401 atgccgaacg ggcggccatg ctggtgcgga catggtttct ggacgaagca acgaaaatga
     8461 atcccaatct gaattttggg caggccgttc tgggcagcag cgatggacgg tgctacggca
     8521 ttattgagac agccgggttt atcggaattg tggacagtgt cggtctaatt caaaacagcc
     8581 ctttttggac agcagctgac cagcagaaac tcgtccagtg gtttacggat tatttgactt
     8641 ggctgaccag ccatccgctg ggtctggaag aaagccagac aaagaataat cattccacat
     8701 ggtatgatgc tcaagcggcg gccttcgccc tctttgtcgg caatcggccg ctggcccagc
     8761 agattcttgc tttagcatca caaaagcata tcgcagcaaa aattgaaccg gacggacgcc
     8821 agccccatga attaacacgt accaaatcgt acggctacag cgtgatgaat ctacgcggaa
     8881 cgtttgtttt ggcccgtctg ggggaacatg tcggcatgga tttgtggaac tgccgaacag
     8941 aagacgaacg ctgcctgcag aaagctctgg attatctgat tttttatgcg gatccaaaaa
     9001 acaaatggcc ttatcagcag attgaaccca tcgacgcttc cgctttgtat ccgctgctga
     9061 aacaggcaca tgctaaaatc cgcggctttg atgctcagcc ggtcttgaaa acactgtccg
     9121 aacagagcat ccattcacag cgttctgccc ttttgtttcc ttaacagcca gcgataatca
     9181 tacttaacaa ttgccgatgc aaaaaatagc tttctaagta tcgatcgcta tcgtcgtgtc
     9241 gcttccttca tcaaccgcgg aaaaaaatat ccttgaaaga gccgccggca aaagagattt
     9301 gtcgctgcct gtgccgtctg agacctgtga atacctgcgc ctatggattt ttagctcatt
     9361 atccataggc acagactgtg agta
//
LOCUS       DAOTBO010000104         9066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_6531_length_9066_cov_5.163911, whole genome shotgun sequence.
ACCESSION   DAOTBO010000104 DAOTBO010000000
VERSION     DAOTBO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6531_length_9066_cov_5.163911"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLE88_10550"
     CDS             complement(<1..314)
                     /locus_tag="PLE88_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76945.1"
                     /translation="MKKHLARFALLPFLSLLVSSIPAYSIVEITYCLELQQAGRWKAT
                     YEVSNSSYLPSIEWFTIFFDYGLYDHLAVETLPPLQMQWQEEIHNPLFAGPLVFPVYY
                     DA"
     gene            complement(1086..3884)
                     /locus_tag="PLE88_10555"
     CDS             complement(1086..3884)
                     /locus_tag="PLE88_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOM76946.1"
                     /translation="MRESNPTDVMRKFPTQLLVSVDTQFLRRLLPACLMLGFFASERF
                     SVSALAAEEVCRSCSCKVSVIGEFSHYKAADSVVIEEAGGEEAAFREEIYGKEFSISV
                     SNLPAGEYKVLIGVAETFFSEPGQRIFSVVSNDMALAADFDIVKAAGKARKVYYITGV
                     IDHEDDALRGPLKVTFKAHRDNAKFNTFELKDASGTSVLFFNASQIADPFTGNAAVIP
                     DIKEAPIWRDPTQPLRKRVEDLIRRMSLAEKVAQLQNDAPAIQRLGLPAYNYWNEALH
                     GVANNGIATVFPAPVGMASTWNPELLRQEGTIIGIEGRAKFNDYASQNNGNSKWWTGL
                     TFWTPNINIFRDPRWGRGQETYGEDPFLTGEIAVEFIKGIQGDHPDYMLAMACAKHYA
                     VHSGLELERHRFNAEPSERDLYETYLPQFERAVCEGKVAGVMSAYNALNGIPCSASTF
                     LLKDLLRTQWGFEGYVVSDCSAINDIWNPRAHRYVQTPEEAAALAVKAGCNLCCGSDY
                     NALVQGVQKGLISEKEIDEALYYTLWTRFRLGLFDPAEKVPYSKYTIADNDKPEHSKV
                     ALEVARQSLVLLKNDGILPLDRSRYKNIAVIGPNGNSKSMLEGNYHGSASHPISILDG
                     IKQLAGDGIKITFAMGSPVTTKHGTAAWSRQDNQTNRPVSELKEEALKIAADAELIIY
                     VGGITPAQEGEGFDRDSIELPEVQTQLIRALYELGKPMVMVNCSGSAVALTWEDEHLS
                     AILQAWYPGQEGGRAVAEVLFGQVNPSGHLPLTFYRATSDLPDFRDYSMANRTYRYFN
                     GKPLYAFGHGLSYTTFEYKNGKLASNVIPADGVATVSFTIENAGRLAGDDVVQIYYRH
                     ISSQVPQPKLALCGFARVHLKQGEAKSVAIDIPAKRLRYWDTEKKQYVVEPGEYELLI
                     GAASDDIRVKLPLTIAGR"
     gene            complement(4016..5827)
                     /locus_tag="PLE88_10560"
     CDS             complement(4016..5827)
                     /locus_tag="PLE88_10560"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM76947.1"
                     /translation="MRTKTNPARQRFLVILITCVLTHVDAESSFQSWAKTPPMGWNSW
                     DCFGTTLTENQAKAQADAMAKYLKPYGWTYFTVDIQWYEPNSQGHIYRRGARLEMDEY
                     SRLIPALTKFPSSANGKGFKPLADYVHSRGLKFGIHIMRGIPKQAVRDNTPILRTQAR
                     AADIANTRSICAWNPDMYGVDMSKPGAQEYYDSLFELYASWGVDFVKVDDIARPYDEV
                     QKAEIEAIRKAIDKAARPMVLSLSPGDTPIERGEHVINHANMWRISDDFWDRWQPLYE
                     MFGRLEKWTQCRAEGAWPDADMLPFGIVEFNRPTRFTPDEQITCMTLWSIARSPLILG
                     ADMTRMDEFTLSLLTNPEVLAVNQASTNNRQISRENDRIVWAADIPGSDDRYVGLFNA
                     QSNDKPFDLSKADYCSRHLRGEPKADIVDICVPIHNARRLVLVVKDGGDGNNYDHAAW
                     IEPTITGPTGSLKLIDLEWSVAAAGWGEVRKNRTVDGRRLTLNGAAVEGIGTHAVSVI
                     EFELPEGYETFTSRGMITEGSQGKGTLEFCVLVNPDKQVKPQYSRVSVSFADLGISGK
                     ARVRNLWTRQDIGVFTGSFDQELPLHGAGLYKISPMP"
     gene            complement(5884..8307)
                     /locus_tag="PLE88_10565"
     CDS             complement(5884..8307)
                     /locus_tag="PLE88_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM76948.1"
                     /translation="MIRNVMKYTILLAVIFLLQEYEVAQGSQQKSVPTKKQTFQPNFE
                     SLEKANPVPEWFRDAKFGIYFHWGVYSVPAFANEWYPRSMYIKGSAENKHHLANYGEV
                     SEWPYHNFIIGAKDKQGRFVQFAPKLKSEGGQFDPDEWAQLFADAGAKFAGPVAEHHD
                     GFSMWASRVNPWNAKDMGPKLDLVGLLTDAIRKKNMKVILSMHHAYNITGFYDVVPKT
                     NDPLLQMLYGQQGKEKNEALWLSKHQEIIQMYQPDIIWQDFNLHIISQPVLLEFLAYY
                     YNKAGEWNKEVVATYKDGLNIKCAVLDYERGGPADITENYWLTDDAVSSSSWCYTEGI
                     RYYSTKQVLHGFLDRISKNGNLLLNISPKADGSIPEEQKDMLLAIGAWLKKYGEAVYA
                     TRAWEQYGEGPTKMGGAHGQMGPPSEGTAGDIRYTRSKDNKTLYAMLLGWEQGQTEVV
                     LKSLSTDRIVGQQPKSVRLINGRAGEYLPLVFKQDAHGLTVCLPERPFEELAYVLRID
                     FDNSIPPLSRHADLDCTPYYHIVPANSTGRWVLGSDFSVTEKTKQAAHQWKLESAGKG
                     VYIISNRQDPRKVIDYNISSHDLILSEYAGKNSQFWKIDDARNGLFTISNKYNPNAKL
                     SLSETAAEGTKAGLDGPLAQWNLVEVCDLKQTAFKPHVIPGTIQAEDFDTGCPGDAFY
                     DSDDKNEGGQYRLNQGVDIETCSAGGYNVGWTRAGEWLAYTIAVSKTGAYRVSFYVAS
                     AQDSPRMHLECGGSDITGIISVPNTGGFQNWQIVTRTVNLEAGERILKLVIDGNYVNL
                     DKMVFEEIE"
     gene            complement(8328..>9066)
                     /locus_tag="PLE88_10570"
     CDS             complement(8328..>9066)
                     /locus_tag="PLE88_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOM76949.1"
                     /translation="PSSYEEGPWFYRRNGKYYMIFAGGPIPEHIGYSTGPSPTGPWTY
                     QGIIMPAQGGSFTNHPGVCDYKGNSYFFYHNGALPGGHGFHRSVCVEQFKYNEDGTIP
                     TLSMTKEGAPPIGHLNPYRTTEAETICWALGIKTERCSEGGMNVCSIENGDYIKVKGV
                     DFGPGAKSFEARVASESQGGRIELRFDSPTGKLAGICSVPCTGGWQKWETKSCTVKDA
                     AGIHDLYFVFAGDSGFLMTFNWWRFER"
BASE COUNT         2245 a         2418 c         2067 g         2336 t
ORIGIN      
        1 tgcgcatcat aatagacagg gaatacaaga ggacctgcaa atagaggatt gtgaatctct
       61 tcctgccatt gcatttgaag cggcggcaat gtctcaacgg ctaaatgatc atagaggccg
      121 taatcaaaaa aaattgtaaa ccattcaatc gacggaaggt acgatgaatt tgaaacctca
      181 taggtcgcct tccatcgccc cgcttgctgc agctccagac aataagtaat ctccacgatg
      241 gagtaagcgg gaatagaact gacaagcagc gacaagaaag gcaaaagagc aaagcgggca
      301 agatgttttt tcatgaccac accacccctt tctaatgtga taggaccggc ttttccgtcc
      361 tatccttgaa aaaccataac gctatattat caaaaattaa aaaaatagca aaataattat
      421 agatgatgtt ttacagccct tcttttcagg aactcacgac ctcttgcatg tcatccgatg
      481 cccgttggga ccgtcttaaa atgccgtaat ttatcgtaac tcattgcccc gtgtagagct
      541 ataaatcgcg atatagagca atttatacct gaaattgacg gcattgattg catcccatag
      601 cacctcattc catctattgt caataataat acactggttt ttttagaggc aaaaacggtc
      661 atctgcttgt gcgcttctat tgtccagata ataaaaaacg gattgagcca gaaggttgac
      721 aggtgccgcg acctcctgaa tgaagtcatt cacgccgttt gtcaactccc caaaccggcc
      781 tgacggccgc cctgtcgcct tggacagctt catcgagacc aagttaaaaa ctaccttggt
      841 ctccttgagg ctttcattag cagagacaac tatatgtatt cagccttccg cggggaagaa
      901 aaaatgctgg tccgatgcgt atgtttatcc cgggaccaga gacataatcg agtaaagcca
      961 tacaagactg aaatacgcaa gatattgttg catctttttt ttaatcagca tcgtcacacc
     1021 atcttcgcaa agagtaagaa cagcttctgt tctgaccagc taacggctgc ggttttagaa
     1081 ttatttcacc ggccggcaat tgttaggggt aatttcaccc ggatatcgtc cgaagcggcc
     1141 ccaatgagta actcatactc ccccggctct actacatact gttttttctc cgtgtcccag
     1201 taacgcagcc gcttggcggg aatatcaatg gcaacagatt ttgcttcccc ctgctttaga
     1261 tgcacgcgag caaagccgca cagggcaagc ttcggctgcg gaacctggga actgatgtgc
     1321 cggtagtaga tctggaccac atcatccccg gcaagtcggc cggcattttc aatcgtgaaa
     1381 gacaccgttg caacaccgtc ggctggaatc acattcgatg cgagtttgcc atttttatat
     1441 tcaaaggtcg tataactcaa gccatgaccg aatgcataaa gcggcttccc attaaaataa
     1501 cgataggtgc ggttggccat cgaatagtcc ctgaagtcgg gcaagtcgga agttgcacgg
     1561 tagaatgtca gcggtaaatg gccggatgga ttaacttggc caaaaagaac ttcagcaact
     1621 gcgcgtccgc cttcctgtcc aggataccag gcctgcagaa tagcagacag atgttcatct
     1681 tcccatgtca gggcaacagc cgacccgctg cagttgacca tgaccatcgg cttgccgagt
     1741 tcataaagcg cccggataag ctgggtttgc acctcaggca gttcgatcga gtctcgatcg
     1801 aagccctcgc cttcctgtgc aggtgttatg ccgccgacat aaataatcaa ttcggcatct
     1861 gcggcaattt tgagtgcctc ttctttaagc tctgaaaccg ggcggttggt ctggttatcc
     1921 tgccgactcc aggccgccgt cccgtgtttg gtagtaaccg ggctgcccat ggcaaatgta
     1981 attttaatac catccccggc caattgtttt atgccatcca gaattgaaat cggatgcgaa
     2041 gccgagccgt gatagttccc ttcaagcatg gactttgaat tgccgttcgg accaataacg
     2101 gcaatgtttt tatatctgga acggtcaagc ggcaggatgc cgtcgttctt gagcagtacc
     2161 aaggattgcc gtgcaacctc cagagcaacc ttgctgtgtt cgggcttgtc attgtcagca
     2221 atagtatact ttgaatacgg caccttttcg gccggatcaa acaaaccaag acgaaaacgt
     2281 gtccataacg tataatacaa cgcctcatcg atttccttct cgctgattaa gcccttttga
     2341 acaccctgta caagggcatt gtaatcgctg ccgcaacaga gattgcaacc ggccttgacc
     2401 gcaagcgccg ctgcttcttc cggagtctga acataccgat gagcccgcgg attccagata
     2461 tcattgatag cactgcaatc cgaaacaaca tatccctcaa atccccattg tgtgcgcagc
     2521 aagtctttga gaagaaatgt actggcgctg cagggaatcc cgttgagggc gttgtaggcg
     2581 ctcattacgc ctgcaacttt tccctcacat accgcccgtt caaactgagg caggtaggtt
     2641 tcatacaaat cgcgttccga aggttcggca ttgaagcgat ggcgttccag ctccaggccg
     2701 ctgtgtaccg cgtaatgttt tgcacaggcc attgccaaca tgtagtcggg atggtccccc
     2761 tggatgccct tgatgaactc cacggcgatc tcgccggtca gaaagggatc ttcgccgtag
     2821 gtttcctggc cgcggcccca gcgcgggtcg cgaaagatat tgatgttcgg cgtccagaaa
     2881 gtcaaaccgg tccaccattt ggaattacca ttattttggg atgcataatc attgaattta
     2941 gcacggccct caataccgat aatcgttcct tcctgacgaa gcagctccgg attccaggtg
     3001 gaagccatcc ccaccggtgc cgggaaaacg gttgcgattc cgttgttggc aacaccgtgc
     3061 agggcttcat tccaatagtt ataagcggga agtccgagcc gctggattgc cggcgcatcg
     3121 ttctgcaatt gtgccacttt ctctgccagc gacatgcggc gaatcaaatc ttcgacgcgt
     3181 ttccggagcg gttgagtcgg gtcacgccag atcggtgcct ctttgatatc cggtatcaca
     3241 gcggcattgc cagtgaaagg gtcggcaatc tgggaagcgt tgaagaacag aacggatgtt
     3301 cccgaagcat ccttcagttc gaatgtgttg aatttggcat tgtctcgatg ggccttaaag
     3361 gttactttca gcggtcccct gagtgcatca tcttcatgat cgatgacacc ggtgatataa
     3421 tatacttttc ttgccttgcc ggcagctttg acaatatcga aatctgcggc taatgccatg
     3481 tcattagaaa ctacactgaa tattcgctgc ccgggttcgc tgaaaaaggt ttctgctaca
     3541 ccaatcagga ccttgtattc tcccgcaggc agattggaaa cactgatact gaactctttg
     3601 ccatagattt cttcacggaa tgccgcttct tcgccgccgg cttcctcgat tacaacagag
     3661 tccgcggctt tgtagtggct aaactctccg ataacactga ccttgcaaga acaggaccgg
     3721 cagacttcct cggccgctaa agctgaaacc gaaaacctct cactcgcaaa aaaacctagc
     3781 atcagacagg ccggcagcag cctccggagg aactgcgtat ccacagaaac aaggagttgt
     3841 gtcggaaatt ttctcattac atccgtcgga tttgattctc tcatagccaa tcctcaaata
     3901 ataagttttg tctttctatc aaacaacaat atttacaatt gatactacat acactattcc
     3961 tgtttgctca ttttacattt cgccgcagcg gcaaaaacgc cgcccacagc ccgcttcacg
     4021 gcatcggtga gatcttatac aatccggcac catgcagcgg caattcctga tcaaatgaac
     4081 cggtaaacac accaatatcc tgacgcgtcc aaaggttgcg tacccgagcc ttaccggaga
     4141 tgccaagatc cgcaaaggat actgacaccc tactgtattg cggctttact tgcttgtcgg
     4201 gattaaccag aacgcaaaat tcgagcgtgc cttttccctg acttccttcc gtaatcatgc
     4261 ccctcgaggt aaaggtttca tatccttcag gcagttcaaa ttcaatcaca gagactgcat
     4321 gtgtcccgat accttcaaca gccgctccgt tcagcgtcag ccgacgcccg tcaactgtcc
     4381 gattcttccg aacctccccc cagcccgccg cggcaacgct ccattccaag tcgattagct
     4441 tcagggaacc ggtgggaccg gttatcgttg gttcgatcca ggcagcatga tcatagttgt
     4501 tgccatcgcc gccgtcttta accaccagca ctaaccgccg ggcattatga atcggcacac
     4561 agatatcaac tatatccgcc ttgggctcgc cccggagatg acggctgcaa taatcggctt
     4621 tggacaggtc aaatggtttg tcgttgctct gcgcattgaa caatccaaca taccggtcat
     4681 cgctgccggg aatatctgcc gcccagacaa tccgatcatt ttctcttgaa atctgccggt
     4741 tgtttgtgct ggcctgattg acggccagca cttcgggatt tgtcagcagg ctgagtgtga
     4801 attcgtccat ccgcgtcata tccgccccga gaatcagcgg tgagcgagca atactccaca
     4861 gggtcataca ggtaatttgt tcatccggag taaaacgcgt aggacgattg aattccacaa
     4921 tcccgaaagg cagcatatca gcatccggcc atgccccttc cgcccggcat tgggtccatt
     4981 tctcaagtcg tccaaacatc tcatacagcg gctgccagcg gtcccagaaa tcatcggata
     5041 tacgccacat attggcgtgg ttgattacat gttcaccccg ctcaatcggc gtatcgcccg
     5101 gcgacagact cagcaccatg ggccgagcgg ctttgtcgat tgccttgcgg atcgcttcga
     5161 tttcggcttt ctgtacctcg tcataagggc gagcgatatc atcaaccttt acaaaatcca
     5221 caccccatga agcatagagt tcaaaaagcg aatcgtaata ttcttgtgcc ccgggtttgc
     5281 tcatatcgac accatacata tccggattcc atgcacagat agaacgggta ttggcaatat
     5341 cggcggctcg ggcctgtgtt cggagaatgg gagtattgtc gcgcacagcc tgtttgggga
     5401 tgccgcgcat gatgtgaata ccgaatttca ggcctcgaga gtgcacatag tcagccagag
     5461 gcttaaagcc tttgccattg gccgaagaag gaaatttcgt cagcgctggt ataagccggc
     5521 tgtactcatc catctccaat cgggcacccc ggcggtaaat atgtccctgc gaattgggtt
     5581 cataccactg gatgtcaacc gtaaaataag tccatccgta cggtttgagg tatttggcca
     5641 tggcatcagc ctgcgccttt gcctggtttt cggtaagagt tgtgccaaaa caatcccagc
     5701 tgttccaccc catcggcggt gttttcgccc aagactggaa agacgactct gcatcaacat
     5761 gcgttaatac acatgtaatc aatataacca aaaaccgttg tctggccgga ttcgtcttag
     5821 ttcgcattat ctttgaagtt ccttcagttg ctttttttgc ggggcctccg acgcacatat
     5881 tctttattct atttcttcaa aaaccatttt gtcaagattc acataattgc cgtcgatgac
     5941 caatttcagt atacgttcac cagcttcaag gttgactgtc cgggtcacaa tttgccaatt
     6001 ctgaaagccc cccgtattgg gaacagaaat gattccggtt atgtccgagc cgccgcattc
     6061 aaggtgcatc ctggggctgt cctgagccga ggcaacatag aatgaaacac ggtatgcacc
     6121 ggtcttgctg accgctattg tatatgccag ccattccccg gcacgggtcc agcccacatt
     6181 ataacctccg gcgctgcatg tttcgatatc cacaccttga ttgagacgat actgcccgcc
     6241 ttcgttttta tcgtcactgt catagaaagc atcgcccgga cagccggtat cgaagtcctc
     6301 ggcctgaatc gttcccggga taacatgcgg cttaaaagct gtctgtttca aatcacacac
     6361 ttccacgagg ttccattgag ccaatggacc gtccagtccc gcttttgtcc cttcagcagc
     6421 cgtttcagaa agggataatt tggcattcgg attatatttg ttggatatgg tgaataatcc
     6481 atttcgggca tcgtcaatct tccagaactg gctgtttttt ccggcatact ccgaaagaat
     6541 aagatcatga ctggatatat tataatcgat cacctttcga ggatcctggc ggttcgatat
     6601 gatgtaaaca cctttgccgg cagactccaa cttccactgg tgggcagcct gtttcgtttt
     6661 ctcagtcacg gaaaaatctg aacccagcac ccagcgccct gtgctgttgg caggaacaat
     6721 gtgataatac ggggtacaat caaggtcagc atgcctgcta agcggcggaa tgctattgtc
     6781 aaaatcgatc cttagtacat aggccagttc ttcgaaagga cgttccggca aacaaacggt
     6841 taatccgtgc gcgtcctgtt tgaaaaccaa tggcaggtat tcgccggcac ggccgttgat
     6901 caaccggacc gatttgggtt gttgaccaac aatcctgtcc gtagacaatg atttaagaac
     6961 cacttcagtc tgtccctgtt cccagcccag cagcatagca taaagcgtct tattgtcttt
     7021 ggatcttgta taacgaatat caccggccgt cccctcgctc ggaggtccca tttggccgtg
     7081 cgctccgccc atttttgtcg ggccttctcc gtattgttcc cacgcacggg tggcatagac
     7141 tgcctcgcca tatttcttaa gccatgcccc tatggccagc agcatatctt tctgttcctc
     7201 cggaatactg ccgtctgctt tgggagatat attcaataag aggttgccgt ttttgctgat
     7261 tctatccaga aaaccgtgaa gcacttgttt tgtggaataa tatcggattc cttccgtgta
     7321 acaccaactt gaggaactga cggcatcatc cgtcagccag taattttcag taatatcggc
     7381 aggtcctccg cgctcataat caagaaccgc gcatttgata ttgaggccat ctttataggt
     7441 ggcgacgact tccttgttcc attcaccggc tttattgtag tagtaagcca ggaactccaa
     7501 aagcaccggc tgtgaaataa tatgcagatt aaaatcctgc caaatgatat cgggctgata
     7561 catctggatg atttcctgat gcttgctcag ccataacgcc tcgttttttt ctttcccctg
     7621 ctgcccataa agcatctgca ataggggatc gtttgttttc ggcaccacat cataaaatcc
     7681 tgtaatatta taggcatgat gcatcgaaag aataaccttc atatttttct ttcgaatggc
     7741 atctgtcagc aagccgacca aatccagctt cggtcccata tccttagcat tccagggatt
     7801 gaccctgctg gcccacatcg aaaagccgtc gtggtgttcc gcgaccggtc cagcaaattt
     7861 agcgccggca tcggcgaaca attgcgccca ttcatcggga tcaaattgac cgccttcgga
     7921 cttgagcttg ggtgcaaatt gtacaaaacg tccctgcttg tcctttgcgc cgatgatgaa
     7981 attgtggtag ggccactcag acacttcacc atagttagcc aggtgatgtt tgttttctgc
     8041 tgatccttta atatacatgc tgcgcggata ccactcgttg gcaaacgccg gaacactata
     8101 aaccccccaa tggaaataga tgccaaactt ggcgtcccgg aaccattccg gtaccggatt
     8161 tgccttctct aaagattcga aattgggctg aaaggtttgc ttctttgtcg ggacagattt
     8221 ttgttgactg ccttgagcaa cttcgtactc ttgaagcaag aaaatgacgg ccagcaatat
     8281 cgtatatttc atgacatttc gaatcataat ctttctcaca ctcctttcta cctctcaaat
     8341 ctccaccaat tgaaagtcat caaaaatccg ctgtcaccgg caaacacgaa atacaagtcg
     8401 tgtatcccgg cggcatcttt tacagtacag gactttgtct cccacttctg ccagccgccg
     8461 gtacaaggaa cggagcagat ccccgccagc ttgccggtcg gactgtcaaa gcggagttca
     8521 atgcggccgc cttggctttc cgaagcgacc ctggcttcaa aggatttggc cccggggcca
     8581 aaatcaacgc ctttgacttt gatataatcg ccgttttcaa tgctgcatac attcattccg
     8641 ccttcactgc agcgttctgt cttaatcccc aatgcccagc agatcgtttc agcttctgtg
     8701 gttcgatagg gattgagatg gccgatcgga ggcgcccctt ccttggtcat actgagggtc
     8761 gggattgtcc cgtcttcatt gtacttaaac tgctcgacgc ataccgaacg gtgaaatcca
     8821 tggccgcccg gcaaagcacc gttatgataa aagaaatagg aattcccctt atagtcacat
     8881 acgcctggat ggtttgtgaa actgccgcct tgtgcaggca taataatacc ctgataagtc
     8941 cagggacctg ttggactggg gcccgttgaa taaccaatat gttccggtat aggaccgccg
     9001 gcaaaaatca tatagtattt tccatttcgc ctataaaacc atgggccttc ctcataggaa
     9061 gagggc
//
LOCUS       DAOTBO010000105         9059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_6534_length_9059_cov_5.100289, whole genome shotgun sequence.
ACCESSION   DAOTBO010000105 DAOTBO010000000
VERSION     DAOTBO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6534_length_9059_cov_5.100289"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLE88_10575"
     CDS             complement(<1..1146)
                     /locus_tag="PLE88_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOM76950.1"
                     /translation="MTSAVPIVNLTEAQKKAVYHKNGPLLVLAGPGSGKTRVITCRIA
                     ALIEAGVAGRHICAITFTNKAAEEMRLRVQQFVPAAGAHISTFHSLCVWILRRYAEYA
                     GIKPNFSIFDVPDQQRCMKEAIKECSVDAANFTPSRMIDYISRLKNDLEDVETFEARA
                     GDYFEKNTAKIYRQYQRILALNNALDFDDLLIRTAMLLRDWPEIRLQLSERFRYLLVD
                     EYQDTNHAQYQIAKGIALAHRNICVTGDPDQSIYRWRGADIENILAFEKDWPEAAVVK
                     LEENFRSTPNILKTADKLIAHNTKRKSKVLIPTRSDGDEVVIQTCDDEAQEAVWVAQT
                     IENLIRNGTDPNEIAVFYRVNAMSRAIEEAFVSRQLPYQMVRGIEFYA"
     gene            1531..3153
                     /locus_tag="PLE88_10580"
     CDS             1531..3153
                     /locus_tag="PLE88_10580"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF010079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component 1"
                     /protein_id="HOM76951.1"
                     /translation="MNDYKRRIEQARPGQIIPMVRDIDITCPVEFFAKLSNYGRSRHC
                     CLLESRDYLTQGDAGELTFGTANPALYLTGTGRYFMIKALTETGSRMLQYFSARPELF
                     SFCQSIRFDSNVIEGHLAANQGILDEEMRLKSVNQMDIIRTVAFAFELASKPFRITCG
                     LMGAISYDFIDQFEKLPTNQRDILHNPDYELYFADNIFLMDHKNSKGYVIVNAVITDG
                     DRDLIYQQAQQCFDQYFDKIKEERPIALFHADTTTAPSTDTQQEEYEAMVKTAQKHIL
                     DGDIFQVVLSRTITEPCPAEPLDVYRKLRTVNPSPYMFYLNTENTILMGASPELNLRV
                     SGTRERIVEIRPIAGTKPRGRKDGKIDPDTDVRYEAELKLDRKELAEHIMLVDLARND
                     IARVAEPGSRIVNEMLIVERYESVMHLVSNVRGRLRNGLDALSAYLATMNMGTLTGAP
                     KIEAMKIIRMLEKTKRGYYGGAVMYLTVDGQFDSCITIRSMQIRNHQAYVRAGAGIVH
                     DSIPRTEFEETEHKAQSCLKAIRMACEDADKG"
     gene            3158..3775
                     /locus_tag="PLE88_10585"
     CDS             3158..3775
                     /locus_tag="PLE88_10585"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOM76952.1"
                     /translation="MKTNVLFIDNFDSFTYNLVDEFAKRQCSAKVYRADVPLEALDTA
                     YQQFHPDLLVISPGPGNPDTAGVTLKAIDFFKDKLPIFGVCLGHQAIIQYFGGVIEHA
                     PEVMHGKPSRITHTGKGIFEGLENPLQAGRYHSLCAFALPECLEKTAEYGSIIMGIEH
                     KELPIYGVQFHPESVLTPTGGKIIENMLNIAHNLKTDRQTSQRRS"
     gene            3775..4812
                     /gene="trpD"
                     /locus_tag="PLE88_10590"
     CDS             3775..4812
                     /gene="trpD"
                     /locus_tag="PLE88_10590"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOM76953.1"
                     /translation="MAKITKYLNIIFEGKDLTFEQAKELLDTIFEGDVADVQIAAFLA
                     AMRIKKATVSEIAGLAVSLRQHAVPVQVSVKPLVDTCGTGGSSVKTFNISTTAALVAA
                     GAGVYIAKHGNRGITSLCGSADVLAALGVNISPGPQRVAECIEKAHIGFMFAPNFHPA
                     MKFVQPIRKSLDFHTAFNILGPLANPAGAQAQVLGISDESLMTRMIEVLKILGLERAM
                     VVHSGGLDEISTMGPTKIMHLENGQIRSSTIDPTEYGFRLANLDDLAGGDAAVNARIV
                     RDLLTCRETGPKKDIVLLNAAAAIWAAGLAKDLVEGIEMADSSISSGNALQCLEKLIE
                     ISNQPMEQQQQ"
     gene            4809..5447
                     /locus_tag="PLE88_10595"
     CDS             4809..5447
                     /locus_tag="PLE88_10595"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOM76954.1"
                     /translation="MISIKVKICGITNLDDAVAAMEMGADILGFNFYPRSPRYIEPRR
                     AEAIIRKLPAFVDTAGVFVNVPARDIRHLTNSGLFNWIQFHGDETPEFCRQFNSWNIQ
                     TIKAVRVRTVDDIQSSLSYPTYALLFDAFDQAHYGGTGKTFDWSLIRDCPRRVFLAGG
                     IHPDNVVKALKMDVYGIDVCSSIEKEPGKKDIEKMKQLFAAIYNYTGQKVRL"
     gene            5444..6619
                     /gene="trpB"
                     /locus_tag="PLE88_10600"
     CDS             5444..6619
                     /gene="trpB"
                     /locus_tag="PLE88_10600"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOM76955.1"
                     /translation="MIQRGRYGRFGGFYVPQVLIPALEDIEAGFLQFRDNPQFISELD
                     DLYRNYAGRPTPLYHAKRFSEYVGFQVYIKREDLLHGGAHKVNNALGQGLLARYMGKK
                     KLIAETGAGQHGLATAMIGALFGMETKIFMGSVDVARQNVNVHKMKLCGAEVISVSGG
                     TCTLKDAINDALRYWTSHVEDTFYLFGTAGGPHPFPMIVRHFQSPIGAEARKQCLDQL
                     GKLPDMVVACVGGGSNAIGIFSGFLNDASVKLVGVEGGGKGLHTHQHCATLTAGSIGI
                     LHGAQTYVLQDENGQILETESISAGLDYPGVGPEHSYLKETGRALYTAVTDVEVLDVF
                     EMFTRFEGIIPALESSHALAYLINQKGKLPKDTAVLVNLSGRGDKDISIVEQHRPMI"
     gene            6628..7419
                     /gene="trpA"
                     /locus_tag="PLE88_10605"
     CDS             6628..7419
                     /gene="trpA"
                     /locus_tag="PLE88_10605"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOM76956.1"
                     /translation="MGIYQKLFSTLNRPALIPFFVIGDPEEQLSLELVKTAIDAGADV
                     LELGIPFSDPVADGPTIQKADIRAMQAGMTPAKALNFIKKVTDYKPLPVGLLVYYNLI
                     FQYGIEAFFADFKAAGGTSVLVADLSIDDADEIVPAATAAGLETVFMATPNTTDKRIR
                     KISEKTTGFIYAVSLMGTTGERSVLSEKIKPLTERLKSLSDKPVCVGFGISSPQHARQ
                     VAAAGADGVIIGSRIINIIEENLKNPAAMKSQIKSFIESIKNALS"
     gene            complement(7507..7866)
                     /locus_tag="PLE88_10610"
     CDS             complement(7507..7866)
                     /locus_tag="PLE88_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76957.1"
                     /translation="MNIQAEQRQSQDIEQSFEERREFTERRRNLVDRRSGFDRRRGPG
                     HRRTPERRAAEEGQMTPEQFEFLMAIDQYKRANQRPFPTWTEVLEVIKAIGYRKVAEP
                     QPLESFRKADKQIPAVE"
     gene            complement(7908..8246)
                     /locus_tag="PLE88_10615"
     CDS             complement(7908..8246)
                     /locus_tag="PLE88_10615"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOM76958.1"
                     /translation="MQRKERKEQGKSAAAAVSVVTVAFAEDMELARQYQQLLEKNQIP
                     AVIRRQTEMAQSGFSDIAILVPEDYLDEAHSLICQQAAGEDFFGMAFDEPYKEEYRDW
                     SSEEDEDQLD"
     gene            8558..8779
                     /locus_tag="PLE88_10620"
     CDS             8558..8779
                     /locus_tag="PLE88_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76959.1"
                     /translation="MAKKKAAKKPAAKKSGKEVLIVASKVKAYVKSKGMMSSSESIGA
                     LNDCVYAILDAAVKRTQANRRSTLKPQDL"
BASE COUNT         2381 a         2173 c         2120 g         2385 t
ORIGIN      
        1 ggcataaaac tcaatacccc gaaccatctg ataaggaagc tgccggctga caaacgcttc
       61 ttcaatagcc cggctcattg catttacccg ataaaaaaca gcgatttcat tcggatctgt
      121 cccatttcga atcagatttt caattgtctg ggccacccat acggcctctt gagcttcatc
      181 atcgcaggtc tggataacaa cttcatcccc atcagaccgg gtaggtatta ggaccttcga
      241 ttttcgctta gtattatgtg caatcagttt gtctgctgtt ttcagaatat tgggcgtcga
      301 gcggaaattt tcttccagtt tgaccaccgc cgcctccggc caatcttttt cgaaagccag
      361 aatattttca atatcggctc cccgccagcg gtaaatcgat tggtccggat cccctgtcac
      421 acagatattt ctatgggcaa gggcaatgcc tttggcaatt tgatactggg cgtgattggt
      481 atcctgatat tcatcgacca gcagataacg gaaacgctcg ctcagctgga gacggatttc
      541 gggccagtcg cgcagcagca tggccgttcg tatcagcaaa tcatcaaagt caagggcatt
      601 gttcagggcg agtattcgct gatattgtcg atatattttt gctgtattct tttcaaaata
      661 atcccccgcg cgggcttcaa atgtttctac atcctccaag tcattcttaa gccggctgat
      721 atagtcgatc attcgcgacg gagtaaaatt agctgcatca acagaacatt cctttatggc
      781 ctctttcata cagcgctgct gatctggaac atcaaaaatg ctgaagttgg gcttgatgcc
      841 ggcatattca gcatagcgac gcagaatcca aacgcacaat gaatgaaatg tactgatatg
      901 agctccagca gccggaacaa actgctgaac acgcagacgc atttcttctg cggccttgtt
      961 tgtaaatgta attgcacaaa tatggcgtcc tgccacaccg gcctctatca gggctgcaat
     1021 ccggcaggta attacacgag ttttgccgct gcccggtccg gcaagaacca acagaggtcc
     1081 attcttgtgg taaactgcct ttttttgtgc ctctgtcagg ttgactatag gaactgcaga
     1141 agtcaattat gcctcatctt tagaaagttc aatctctctc tgtttacccg aaagaatgaa
     1201 cattaaaaaa gattggatta caaaagtcaa cacaatccct gattcctata aatacggatt
     1261 tttccgaaga aaaaaacgtt tttgaaaatg tttaaaaatt tttcataaaa aagcataatt
     1321 accgtattga cacccgtatg ttatcctgct aaaatgcttc tatgcataga aacgtttatc
     1381 catgctgtgg tttttgctgg cgttggcctg tcgtataaat cggcaggcgc agaagatttt
     1441 ttgtatctct tatcggtatt tatcagcgac ctgcggattt tggagtaatc ccgcaggttt
     1501 ttttatatcg tctatcaaca gaggtcttct atgaatgatt ataaacggcg tatcgagcaa
     1561 gcccggccgg gacagatcat cccgatggtg agggatattg acattacctg tccagtcgag
     1621 ttttttgcca agctgagcaa ttatgggcgc agccggcatt gctgtcttct tgaatcgcgg
     1681 gactacctca ctcaaggaga tgccggcgaa ttgacttttg gaaccgcaaa tcctgctctg
     1741 tatctaactg gtacgggcag gtattttatg attaaagccc tcaccgagac aggcagccgg
     1801 atgcttcaat acttttctgc cagaccggaa ctgttttcat tctgccaatc aatccgcttt
     1861 gattctaatg ttatcgaagg gcatctcgct gccaatcaag gtattctgga tgaagaaatg
     1921 cgtctgaaaa gcgtcaacca aatggatatc attcggactg ttgcgtttgc ttttgaatta
     1981 gccagcaaac ccttccgcat cacctgtggt ctgatgggag caatcagtta tgattttatt
     2041 gaccaatttg aaaaactgcc taccaaccag cgggatatcc tgcataatcc tgactatgag
     2101 ctgtattttg cagacaatat ttttctgatg gatcataaaa actcaaaggg ctatgtcatt
     2161 gtaaatgcgg taatcaccga cggtgaccgt gatttaattt accagcaggc tcagcagtgt
     2221 ttcgaccaat acttcgacaa aatcaaagaa gaacgtccca tcgccttgtt tcacgcagat
     2281 acaacaacag ctccgtctac cgatacgcag caggaggagt acgaagcaat ggtcaaaact
     2341 gcccagaaac atattcttga cggagatatt tttcaggttg ttttgagccg tacaatcact
     2401 gagccgtgtc cagctgagcc gctggatgtc taccggaagc ttcgaactgt caatccttcc
     2461 ccgtatatgt tttatctgaa caccgagaat accattctga tgggagccag ccctgaactg
     2521 aacttgcggg tcagcgggac acgggaacgg attgttgaaa tccgtcctat tgcaggcaca
     2581 aaaccccggg gccgtaaaga cggcaaaatc gatcctgata ctgatgtacg gtatgaagcg
     2641 gaattaaagc tggaccgcaa agagctggcc gaacatatta tgctggttga tttggcgcgg
     2701 aatgacattg cccgcgttgc cgagccaggc agccgtatcg ttaatgaaat gctgatcgtc
     2761 gagcggtatg aatctgtgat gcatcttgtc agcaacgtgc ggggacgcct gcgaaacggc
     2821 ttggacgcct tgagtgccta tttggccaca atgaatatgg gcacgctgac cggtgcgccc
     2881 aaaattgaag caatgaaaat catccgcatg cttgaaaaaa ccaaacgcgg ctactacggc
     2941 ggcgctgtaa tgtatctgac tgtcgacggc caatttgaca gctgcatcac catccgcagc
     3001 atgcagattc gcaaccatca ggcctatgta cgggccggcg cgggtattgt acatgacagt
     3061 attcccagaa ccgaatttga agaaacagaa cacaaggcac agtcatgcct gaaagctatc
     3121 cgcatggcct gtgaagatgc cgataaggga tgagacaatg aaaaccaatg ttttgtttat
     3181 tgataatttt gattcgttta cctataacct tgtagatgag tttgccaagc gccaatgttc
     3241 tgcaaaagtc tatcgtgctg atgttccgct ggaagcgctc gatacagcat accagcagtt
     3301 ccatccggac ttattggtta tttcccccgg tcccggcaat cccgacacag ccggcgtgac
     3361 gctgaaggca attgattttt ttaaggacaa actgccgatt ttcggcgttt gtctcggcca
     3421 tcaggcaatc attcagtact tcgggggtgt tattgagcat gctcccgaag tgatgcacgg
     3481 caagccgtcc cgaattaccc acaccggaaa aggcattttc gaggggcttg agaatcccct
     3541 ccaggcaggc cggtatcaca gtttatgcgc ttttgccctg cctgaatgtc tggaaaagac
     3601 cgctgaatac ggcagcatta tcatgggtat tgagcacaaa gagcttccaa tctatggtgt
     3661 gcagttccat ccagaaagcg ttttaacgcc tacaggcgga aaaattattg aaaatatgct
     3721 caacattgcc cacaatctca aaaccgatcg gcagaccagc caaaggaggt cttaatggca
     3781 aaaataacaa aatatctaaa tattattttt gaaggaaaag acttaacatt tgaacaggca
     3841 aaagaactgc tggacacaat ctttgaaggg gatgtggcgg atgttcagat tgcggccttt
     3901 ttggcggcga tgcggataaa aaaggcaacc gtttcagaaa ttgccgggct ggctgtttca
     3961 ctccgccaac acgcagtacc tgttcaggtc agcgtcaaac cgttggtcga tacctgcggc
     4021 actggcggtt cttcggttaa aacatttaat atctctacta cggctgcttt ggttgctgcc
     4081 ggtgcgggcg tttatatcgc caaacacggc aaccgcggca tcaccagcct gtgcggctcg
     4141 gctgatgttc tggcagcttt gggagtgaat atctcacccg ggccgcagcg cgttgctgaa
     4201 tgtatcgaaa aggcccatat cggctttatg ttcgcaccca attttcatcc ggcaatgaaa
     4261 tttgtgcagc caatccggaa aagtctggac tttcatacgg cctttaatat ccttggaccg
     4321 ctggcaaatc ccgccggggc tcaagcccaa gtgctgggta tttcagatga gtccctgatg
     4381 acgcgtatga ttgaagttct taaaatttta ggattagaaa gggctatggt tgttcacagc
     4441 ggcggcttgg atgaaatcag cacaatggga cccactaaaa taatgcatct ggaaaacggg
     4501 caaatccgtt ccagtacgat tgacccgact gaatacggtt tccgactggc aaatttggat
     4561 gaccttgccg gaggggatgc tgctgtcaat gctcgaattg ttcgcgacct gctgacctgc
     4621 cgtgaaacgg gacctaaaaa agatattgtg ctgctgaatg ccgctgctgc catttgggct
     4681 gcgggtcttg caaaagatct tgtggaaggc attgaaatgg ctgactcttc catttcatcc
     4741 ggcaatgcct tacaatgtct tgaaaaacta attgaaatca gcaaccagcc gatggagcag
     4801 caacagcaat gatcagcata aaagttaaga tctgcggcat taccaatctg gatgatgccg
     4861 tagcagctat ggaaatggga gcagatattc tcggattcaa tttttaccca cgaagccccc
     4921 gctatattga gcctcggcga gcagaagcca tcattcgcaa gctgccggct tttgtagaca
     4981 cagccggtgt attcgtaaat gtcccggcac gtgacattcg gcatctgaca aattcgggtt
     5041 tattcaattg gattcagttc catggcgacg aaacaccgga gttttgccgc cagtttaaca
     5101 gctggaatat ccaaacaatt aaggccgtcc gagtacggac tgtcgacgat atacaaagtt
     5161 ccttgtctta tccgacctat gccctgctgt ttgatgcctt tgatcaggca cactatggcg
     5221 gaacaggcaa aacttttgac tggtccttaa tccgggattg tccacgaagg gtttttttgg
     5281 ccggcggcat tcatcccgat aatgtcgtca aggccttaaa aatggacgtg tacggcattg
     5341 atgtatgcag cagcattgag aaagaaccgg gcaaaaaaga tattgaaaaa atgaaacagc
     5401 tttttgcggc gatttacaac tatacgggac aaaaggtgcg attatgatac aacgcggacg
     5461 ctacggccgt ttcggcggct tttatgtacc ccaagtgctg attccagcat tggaagatat
     5521 cgaagcaggt tttttgcaat ttcgcgacaa tcctcaattt atatctgaac tggatgacct
     5581 gtaccggaac tatgcaggac ggccaactcc cctttatcat gcaaaacggt ttagtgagta
     5641 tgtcggcttt caggtttaca tcaaacgaga agatttgctt cacggcgggg ctcacaaggt
     5701 aaataatgct cttggacagg gacttttagc acggtatatg ggcaaaaaga aactcatcgc
     5761 cgaaaccgga gccggtcagc acgggctggc tacagctatg atcggcgcct tgttcggcat
     5821 ggaaaccaaa atctttatgg gctcggtcga tgtagcccgc caaaatgtca atgttcacaa
     5881 gatgaaacta tgcggtgccg aagtcatctc tgtttcagga ggcacttgca cgctgaagga
     5941 tgccattaac gatgctctgc gatactggac atctcatgta gaagatacat tttacctttt
     6001 cggcacagca ggcgggccac acccgtttcc gatgattgta cgccactttc agtcgcctat
     6061 tggtgcagaa gcccgcaaac aatgcctcga ccaactaggt aaacttccgg atatggttgt
     6121 cgcctgtgtt gggggcggct cgaatgcgat cggtatcttt tccggctttt taaatgacgc
     6181 ctctgtcaaa ctggtggggg ttgaaggcgg cggcaaggga ctgcataccc atcaacactg
     6241 cgctacgctg acggccggaa gcatcggcat actgcatggg gcacaaacct atgtactgca
     6301 ggatgaaaat ggtcagattc ttgaaaccga atccatctct gccggccttg actatcccgg
     6361 agtcgggccg gaacattcgt atctcaaaga aaccggcaga gctttatata cggcggttac
     6421 cgatgtggaa gttttggatg ttttcgagat gtttacccga tttgagggca tcatcccggc
     6481 ccttgaaagc tcccatgcct tggcttattt aatcaatcag aaaggtaaac tgccaaagga
     6541 tacagccgtg ctggtcaatc tttccggccg cggcgacaaa gacatctcaa ttgttgaaca
     6601 gcaccgaccg atgatatgag gacttctatg ggtatttatc aaaaactttt cagcacactc
     6661 aaccgtcctg cgttgatacc cttttttgtg attggagacc ccgaggagca gctttctctc
     6721 gaactggtta aaaccgctat tgacgccgga gccgatgttc tggagctggg tatccctttt
     6781 tccgatcctg tagccgacgg tccaaccatc cagaaggccg acatccgagc aatgcaggcg
     6841 ggtatgacgc cggcaaaggc acttaacttc atcaaaaagg ttacggacta taaacctttg
     6901 ccggtaggtt tgctggtgta ttacaacctg attttccaat atggcattga ggcatttttt
     6961 gctgatttca aggccgccgg cggaaccagc gttctggtgg cagatttaag cattgacgat
     7021 gccgatgaaa tagttccagc cgcaacagca gccggcttgg aaacagtttt tatggccaca
     7081 cccaatacca cagataaacg catccgcaaa atcagcgaga aaacaaccgg ttttatctac
     7141 gccgtttccc tgatgggaac cacaggtgaa agaagtgttc tttcggaaaa gattaaaccc
     7201 ctcacagagc ggcttaaatc cctcagcgat aaacccgttt gcgtaggttt tggcatcagc
     7261 agcccgcaac atgcccgtca ggttgccgcc gccggcgcgg atggtgtgat tatcggcagc
     7321 cgaatcatca acattatcga agaaaaccta aagaatccgg ccgcaatgaa atcccaaata
     7381 aaatccttca tcgaatctat aaaaaatgct ctcagctgat accgctgccc gcagacagtc
     7441 tggccgttta aaactgaacc ctttggggct ttccttattt gaataatccc aaccggcggc
     7501 tgcccattac tcgacagcgg gaatctgctt atctgccttg cggaaggatt cgagcggctg
     7561 cggttcggct actttacgat agccgattgc tttgattact tccagtacct ctgtccaagt
     7621 tggaaaaggc ctctgattag cccgtttata ctgatcgata gccattagga attcaaactg
     7681 ttccggcgtc atctgccctt cctcggcagc ccgtcgttcg ggggtgcggc gatgaccggg
     7741 gccgcgccgg cggtcaaagc cgctgcgccg atccacgaga tttctccgcc gttctgtgaa
     7801 ttcccgtctt tcttcaaaac tctgctcgat atcttgagac tgtctttgtt ctgcctgtat
     7861 gttcattata ttctctcttg acattttata aaaagctgct gattatctta atccagctgg
     7921 tcttcatctt cctctgaaga ccaatcccga tattcttctt tatacggttc atcaaaagcc
     7981 atcccgaaga aatcctcccc ggcagcctgc tgacaaatca gcgaatgggc ctcatccaga
     8041 tagtcttctg gaaccagtat ggctatatcc gaaaagccgc tctgggccat ctcggtctgc
     8101 cgcctgataa cagccggaat ctgattcttt tcaagaagct gctgatactg gcgggcaagt
     8161 tccatatctt cggcaaacgc aacggttaca acagataccg cggcggctgc agatttgcct
     8221 tgttcttttc gttcttttcg ctgcattatt ttaatctcca attcgctgcc caacacttaa
     8281 ctgcacataa gcggcaatga catcggctat tttgtgggat cgattaacaa aaaaggattt
     8341 gaaaatggca tagaatgcat ccttaggccg ttttgagagg tcagatttta ccgatttttc
     8401 ggtttatacg ccctatgcat gcaaaatttt gcagtttttc gagatttttt tattgcaaag
     8461 gggctgtatt cttttcaaaa taagcatcta aatgccgttc tggccatttc gaggccgatt
     8521 ttattggaac tattaacttt ttacatggag gtgccacatg gcaaagaaaa aagcagcaaa
     8581 gaaaccggca gcaaagaaaa gcggcaaaga agtcctgatc gtagccagca aagtaaaggc
     8641 atacgtcaag agcaaaggga tgatgagttc ctccgaatcc atcggcgctc tgaatgactg
     8701 tgtttatgcc atactcgatg cggctgtcaa acggacacag gcaaatcgcc gcagcacact
     8761 caagccgcag gatctataag ccctgtttgc tggaaacaat tcctcaaaga ccgctttcat
     8821 actatggaag cggtcttttt ttctcctcat ttccaaacaa gaatacgaca aaacaattcg
     8881 ccttttttcc tgtatttttt gttatttgct tgaattttta acatatttta ggaaaaattt
     8941 tgatagacgc cgcgccttct tgtatttata attttagcaa atcgtttctt tgaagaagaa
     9001 agaagggcgt acaaatacgc aggaaaaatg gactttgact gttcagttta ttggaaagc
//
LOCUS       DAOTBO010000106         8992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_6599_length_8992_cov_5.211704, whole genome shotgun sequence.
ACCESSION   DAOTBO010000106 DAOTBO010000000
VERSION     DAOTBO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_8992_cov_5.211704"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLE88_10625"
     CDS             <1..308
                     /locus_tag="PLE88_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76960.1"
                     /translation="PGQPDRWCTIWHIAGYLGVDPHPLTLRQLLWMAEGHHRDAWQHT
                     ASIMALIANVNRDPKKTRAFKPSDFNPYVPKTSRPDVIVVTKENISQLKHAFIGDKK"
     gene            305..829
                     /locus_tag="PLE88_10630"
     CDS             305..829
                     /locus_tag="PLE88_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76961.1"
                     /translation="MKNDVVHSVFNWLDHNRYTVASVLVFILTMGIVVGLTGCESATT
                     GLTVAADGTATKVSRSEFERQALVGEKDFAVRRIELDGLAATFNEEIKAFNERIQAGL
                     NDLQRHDEFKQQLFDTIGVVAIGATEGTLNPTALVPIGIGLLGGALGLGTSADNRRKD
                     KVITDLKVNVLATT"
     gene            832..1608
                     /locus_tag="PLE88_10635"
     CDS             832..1608
                     /locus_tag="PLE88_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76962.1"
                     /translation="MIAVDTVPIQQAKQLCRDYHYSDVFPPHCMVSLGFYDEQGLGGV
                     AIWGWGVRPRHTIQLLFPSLDTSDYWELCRLCCREDLPRNTESQLLAGCAEWFRQKQS
                     ERQVLYTWADGIRGKPGYVYQAANWLYGGFINTEIYLTENHEPVHPRLMITRFGSRSK
                     AVWTGLGLRRIRGKQFRYCQFLCGHKQRKQLLKESPINWTRHYPKHDDLVWRIDAGEG
                     SRETRNPLRIERSGQFRQSAVKSFQRPAPSRQLIGQGSLF"
     gene            1653..3644
                     /locus_tag="PLE88_10640"
     CDS             1653..3644
                     /locus_tag="PLE88_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOM76963.1"
                     /translation="MANTGAIRAGRAFVELFADNTQLVRGLRAAERKLRAFGDSIRTM
                     GLKMMSIGAAMLAPLAGSAKVFSTMGDSVAKMAKRTGLSVESVSELSYAASQSGIEIE
                     ALENGLRPMQRSIYDAGRGLSTAVDALSDLGLSYKQLENLSPEEQFKVIAERLNGIQD
                     PTKKAAVAMTLLGRSGTALLPLFEQGAAGIDALQQEARRLGLTMSSEDARAAEVFGDT
                     LDRLWSVVKMTTFHIGAALVPALQKMVEAVIDVVVKISAWIQQNRAVIVMIAKFAAAL
                     VAGGAAMVVLGTTISGLGLAFGKLSAIITGIGAVFKTLGSVIAFLTSPIGLVIAAVAA
                     LGAYLIYTTDTAGKAVEWLGQKFSILKDDAMTAYQGIADAMAAGDMALAAKILWLTLK
                     MEWTRGVNVLEKAWLDFRNFFIRIGYDAFYGMIAAAQTVWHGLEVGWIETTAFFSKCW
                     QGFVSFFAKSWENIKAGAQKTWNWIKSLFDDSFDLQTENKMVEDQRQQAIAAIEDEQQ
                     RKLAEREAKRQSQRDQAARMNDATLEEIGRQHAAKYQALDAEYDARMAENTRDLEAAR
                     KEWKDSIQRAKDKRTDKELERSAAPGFEKANTAWMDMGDALTEAANKISVTGTFNAAA
                     AWGLGTGNAADRTAKATEETARNTKRILDETKNGAARFT"
     gene            3751..4542
                     /locus_tag="PLE88_10645"
     CDS             3751..4542
                     /locus_tag="PLE88_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76964.1"
                     /translation="MSITVTEKFESRDVVRGSNPSAQLNFVVAGTDDHDLALIELATV
                     APTVFDSLPRLNYGVEPIAETLWLGFVRYGYQTIQQTGSTVYQFDTGGGTQHITQSLA
                     TVSRYGRPGFTAPNFFGAIGVTGGSVDGVDITVPVYSFSEVIYMDDSDVTDAYKATLF
                     ALTGRVNNAAWRNYAAGEVLFLGASGSKRGSDDWELSFRFAASPNMTGLTIGDITGIA
                     KQGWHYLWVQYIDQEDTTAKSLVKRPLSVHIEQVYPYGNFALLGV"
     gene            4546..5331
                     /locus_tag="PLE88_10650"
     CDS             4546..5331
                     /locus_tag="PLE88_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76965.1"
                     /translation="MGDSLKKVTSGDPLRIPAQAYNLFIDTARQYLASKQNAGVRPHP
                     ASVNDTRVLVKNNSGADCAQFAVLGIDGIIFDPADALEAFKQQVVVSGTTPSSTTHSD
                     GRFVILNEPIVAGNLGYGWIVGVCQVQVNVTSESHRYADITNGSSASLVSADAGPCVI
                     LWKPLGTGLLWSIVRLGASGSGLHIAFAKTDAGAANSIDCYLDTNGTGPVVTVTCPIC
                     GGSALNLAFPRLAIGDPITVWHDGTIWRSVMTFQAADEDCQDV"
     gene            5324..6802
                     /locus_tag="PLE88_10655"
     CDS             5324..6802
                     /locus_tag="PLE88_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76966.1"
                     /translation="MYKLPKYNLNTGRLFSRNGKPTCRCCGPVCLSELFMPSQYLCSF
                     RYKKYIDDAPPYLKKEILTAYKVHTGTIGYIGWKPISKYPASGYWSYEDNWMWFGPHV
                     CWIQESFSASNPTAKFIVTAIPSGQRIAQIVDTFSWDRSLNYNGALSAISGQANMCNV
                     SRTFYDVIPTADQLTGGYVKSWNFVYSDSGVNANTVNSDVSGMGTLVEQITETQYATY
                     TPCGGVKMARNTTSPYPAHPAVIGDEVFVSVACDSTMYYEGGSMYRGRGWCTENPFSA
                     FAKMNLTTGQVSWFGSWYGGQLGSSVPTRWVMRILGIDSPSVVFVDNYNYLVRKDDAD
                     YLDWLEKGKYVWRVSSSGLNLGCYASMGGFLYTGNPSSNTASKIDLSNGSFMDITSAI
                     GGAIIGRYGDYLRVRNNAYMDQEYLVDPNNFANKRAIQPAGVLLYPSCNHAECVSWGY
                     DRLNTVLRDSGDSRVGMGDLHVYDDWDMGSFSSVLDLTAVEF"
     gene            6802..7143
                     /locus_tag="PLE88_10660"
     CDS             6802..7143
                     /locus_tag="PLE88_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76967.1"
                     /translation="MSCCGKTIRKVIHIAKGTMADMLGIKYEFTDGRVRTCRNCENRT
                     WLSASEYWEWVADHGGRMKFMAELDDLSSWPPLPKAEYQVGNLLYCRICKCRITRKAR
                     IEAEKCPQGNW"
     gene            7469..7690
                     /locus_tag="PLE88_10665"
     CDS             7469..7690
                     /locus_tag="PLE88_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76968.1"
                     /translation="MIRFDCSKCEHNYRVPDEYAGKRIRCKACDTVITIPAPETETVK
                     CGDSIATYNRLLEEMSKYEKQAPAIDSES"
     gene            7919..>8992
                     /locus_tag="PLE88_10670"
     CDS             7919..>8992
                     /locus_tag="PLE88_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOM76969.1"
                     /translation="MSDKEASVNVGKNLNGVIKIDAAQIHSHLDSMVRSTVEETLNKL
                     LDEEADRLCNAGRYEHSDSRTDTRAGHYPRTYHAKVGPLTLKIPKLRKTTFETAIIER
                     YRRREASVEEALIEMYLAGVSVRRIEDITEALWGTKVSPGTVSELNKKIYSQIELWRN
                     RPLEGSYAYVYLDGIYLKRSWGGEVRNIAVLVAVGVDSLGFREILGVCEGIKEDQESW
                     RSFLRHLKQRGLTGVQLVISDKCLGLVETLGEFFADARWQRCTVHFYRNVFSCVPNDK
                     VKAVAGMLKAIHAQESRDAALQKVAAVVERLVAMKLSKAAETLRAGIAETLSYYDFPR
                     EHWIRIDCPPGLGPFRVLGFPTLI"
BASE COUNT         2107 a         2175 c         2565 g         2145 t
ORIGIN      
        1 gacctggaca acctgatcga tggtgcacta tctggcacat tgccggatac ctcggggtcg
       61 atccccatcc cctgacgctg cggcaactct tgtggatggc cgaaggtcat catcgcgatg
      121 cctggcagca cacagcctct atcatggcgc tgattgctaa tgtcaatcgt gacccaaaaa
      181 aaactcgggc ctttaagcca tcagacttta acccctatgt tccaaaaaca tcccggccgg
      241 atgtgattgt cgttacgaaa gaaaatattt cacaactcaa acacgctttt attggagaca
      301 aaaaatgaaa aatgatgtag tacactcggt attcaattgg ctggatcaca atcgttacac
      361 cgttgcatcg gtgctggtct ttatcctgac gatgggcatt gtggtgggac tgaccggctg
      421 tgaatcggcg accaccgggc tgaccgttgc cgctgacggc actgcgacca aagtttcccg
      481 cagcgaattc gagcgacagg cgctggtcgg cgagaaagat ttcgccgttc gacgcatcga
      541 actggatgga ttggccgcca cattcaatga agagatcaag gcatttaatg aacgtatcca
      601 ggcgggactg aacgatctgc agcgacatga tgagtttaag cagcagcttt tcgacacgat
      661 tggagtcgtt gccatcggcg ctaccgaagg gacgctgaat cccaccgctt tagttccgat
      721 tggaatcggt ttattgggcg gtgcattggg gttgggtacg tctgccgaca accgacgtaa
      781 ggataaggtc atcaccgacc tgaaagtcaa cgtcttggcg accacgtaac catgattgca
      841 gtcgacacag ttcccatcca gcaggccaaa caattgtgca gggactatca ctacagtgat
      901 gtttttccgc cgcactgcat ggtatctctg ggcttctatg atgaacaggg cctgggtggt
      961 gtggccatct ggggctgggg tgtgcggccc aggcacacca ttcagctgtt gttcccctcg
     1021 ctggatacgt cggattattg ggaactgtgt cggctgtgct gccgggagga tttgccgcgt
     1081 aataccgaaa gccaattgct cgctgggtgt gcggagtggt tccgccagaa acaatccgag
     1141 cggcaggtac tttatacctg ggccgatggt attcgcggca aacccggcta tgtttatcag
     1201 gctgctaatt ggctctatgg cgggtttatc aatacggaaa tctatctgac cgaaaatcat
     1261 gaaccggtcc atccgcgatt gatgattacg cgattcggca gccgcagcaa agcggtctgg
     1321 acggggttgg gccttcggcg gattcgcggg aagcaatttc ggtactgtca gttcttatgc
     1381 ggtcataaac agcgaaagca actactcaag gaaagtccca tcaactggac caggcattat
     1441 cccaagcatg acgatttagt gtggcgtatt gatgcgggcg aggggtcaag agagacccgc
     1501 aatcccctca ggattgagag gtcggggcag ttccggcagt ccgctgtgaa gagcttccag
     1561 cgaccagcac ccagccgcca gcttattgga cagggaagcc tgttttaaag ctgggagctg
     1621 gaaactggca gctaaaaaat cggagatttt tcatggctaa tacgggtgca attcgggcgg
     1681 gtcgagcgtt tgtcgagcta tttgccgaca atacccaatt agtgcggggc ttacgggctg
     1741 cagaacgcaa actccgcgcc ttcggcgatt ctatccgcac aatgggtctt aaaatgatgt
     1801 ccattggcgc tgccatgttg gcgcctctgg ccggctctgc taaggtattc agtaccatgg
     1861 gcgactccgt cgccaagatg gccaaacgga cgggtctgtc ggtcgaatct gtcagtgagc
     1921 tttcttatgc cgccagccag agtggtatcg aaatcgaggc cctggaaaac ggtcttcgcc
     1981 ccatgcagcg gagcatttat gacgccggcc ggggcctcag caccgctgtg gatgcattat
     2041 ccgatttagg actctcttat aagcaattgg agaatctatc tccggaagag caatttaagg
     2101 ttatcgcaga acggctcaat ggcatccagg acccgactaa aaaggccgct gtggcgatga
     2161 cacttctcgg ccgttcagga acggcgctgt tgcccctgtt tgaacaggga gctgccggga
     2221 ttgatgccct gcagcaggag gctcggcggt tgggtcttac gatgagcagt gaagatgcac
     2281 gggctgcaga ggtctttggc gacaccttag accgcctctg gtcggtcgtt aagatgacga
     2341 ccttccatat tggcgctgcc ttagttcctg ctctgcaaaa aatggtcgag gcggtaattg
     2401 atgtggttgt taagatcagc gcctggatac aacaaaaccg ggctgtcatt gtcatgattg
     2461 ccaaattcgc tgcagcgctg gttgccggcg gtgcggcgat ggtggttttg ggcacaacga
     2521 tcagtggctt aggtctggcc ttcggcaaac tcagtgccat tattaccggt atcggcgctg
     2581 tgttcaagac actcggttct gtgattgcgt ttttgacatc gcccatcggt cttgtcattg
     2641 ccgctgtcgc tgccttgggt gcatatctta tctatacgac ggacacagcg ggtaaagcgg
     2701 ttgaatggct gggccagaag ttcagtattc tcaaagacga tgccatgact gcctaccagg
     2761 ggattgctga cgccatggca gcaggcgata tggcgctggc ggctaaaatc ctctggctca
     2821 cactcaagat ggagtggaca cggggtgtta atgtcttgga aaaggcgtgg ctggactttc
     2881 gcaacttctt tatccgtatc ggctatgacg ccttctatgg catgattgca gcagcacaga
     2941 ctgtctggca cggtctggag gtcggttgga ttgagacgac agccttcttt agtaaatgct
     3001 ggcagggctt tgtatcgttc tttgccaaga gctgggaaaa catcaaggcc ggggcacaga
     3061 agacttggaa ctggataaaa tcactgtttg atgactcctt tgacctccag actgaaaaca
     3121 aaatggtgga ggaccagcgt cagcaggcga ttgccgctat cgaggacgaa cagcagcgaa
     3181 aattagccga gcgcgaggca aaacgtcagt cccagcggga ccaggcggca cggatgaatg
     3241 atgcgaccct ggaggagatt ggccgccagc atgccgccaa ataccaggcc ctcgatgctg
     3301 aatacgatgc ccgtatggct gagaacaccc gggatttaga ggccgcacgg aaggaatgga
     3361 aagactccat ccagcgggct aaagataaac gcacagataa ggaactcgaa agaagcgccg
     3421 cccctggctt tgagaaagcc aacactgcct ggatggatat gggcgatgca ctgaccgagg
     3481 cggctaataa aatcagtgtg acgggaacct ttaacgccgc agccgcctgg ggtctaggta
     3541 cgggtaatgc tgctgacagg acggctaagg cgactgagga gaccgcccgc aacaccaaac
     3601 gtatcctgga cgagacgaaa aacggtgcgg cgaggtttac atgaaatcag acttgagact
     3661 cgaggcgtga gacttgagca cataaaaaga atatgccttt cttcctcagg cctcaagcct
     3721 ctggtctcag gtctcaaatg agcaacgcga atgtcaatta ccgttacaga aaaattcgaa
     3781 tcccgcgatg tcgtccgagg cagcaatccg tcggcacaat tgaactttgt ggttgccggc
     3841 accgacgacc acgacctggc gctgattgaa ttggcgacgg ttgcgccgac cgtttttgac
     3901 agcctgcccc gtctcaatta tggcgtcgaa cccatcgccg agaccctctg gctgggcttt
     3961 gtccgttatg gctaccagac tatccaacag accggctcga ctgtctatca attcgatacc
     4021 ggcgggggga ctcagcatat tacccagagt ttggcgactg tatctcgcta cggacggccg
     4081 ggatttacag caccgaattt ttttggggcg attggtgtca ccggcggcag cgtcgatggc
     4141 gtcgatatta cggtgcccgt ctatagcttc agtgaagtta tatacatgga cgattcggat
     4201 gtcaccgacg cctacaaagc gacacttttt gccctgacgg gtcgggttaa taatgccgcc
     4261 tggcgcaact atgcagcggg ggaggtcttg ttcctgggtg cctccggctc aaaacgcggc
     4321 tctgacgatt gggagctctc ctttcgcttt gccgccagcc ccaatatgac ggggcttacc
     4381 atcggtgata ttacggggat tgccaaacag ggctggcatt atctgtgggt ccagtatatc
     4441 gaccaggagg atacaaccgc taaatccctc gtcaaacggc ccttgtcagt ccatatcgag
     4501 caggtgtatc cgtatggcaa ctttgcctta ttgggagttt gacggatggg tgactcgcta
     4561 aaaaaggtaa cttcaggcga tccgctgcgg attccggccc aagcctataa cctctttatc
     4621 gacacagccc gtcagtacct ggccagcaag cagaatgccg gcgtgcgtcc ccatccggca
     4681 tctgtcaacg acacccgcgt gctcgttaag aacaacagcg gtgctgattg tgcccaattt
     4741 gccgtccttg gaattgacgg gattatcttt gaccctgccg atgcactgga ggccttcaaa
     4801 cagcaggtgg ttgtcagcgg cacaacacca tcgagtacta ctcattcaga tgggcggttt
     4861 gtcatactga acgagcccat tgtggccggc aatctgggat atggctggat tgtcggtgtc
     4921 tgtcaggttc aggtcaatgt tacgagtgaa tctcaccgtt atgccgacat taccaatggc
     4981 tcgagtgcct cccttgtaag tgccgacgcc ggtccgtgtg tcattctctg gaagccgctc
     5041 gggacgggtc tattgtggtc tatcgttcgt ttaggggcaa gcggcagcgg tcttcacatt
     5101 gcctttgcca aaacggatgc aggggcggcc aattccatag attgctactt agacaccaat
     5161 gggaccggtc cggttgttac ggtgacctgc cccatttgcg gggggagcgc cttgaatctg
     5221 gcattcccgc gtcttgctat aggtgacccg attacggtgt ggcatgatgg cacaatctgg
     5281 cgttcagtca tgacgttcca ggcggccgat gaggattgtc aggatgtata aactgcccaa
     5341 atacaacctg aataccggac ggctgttttc ccgaaacggc aaacccacct gtcgctgctg
     5401 cggacccgtg tgtttgtccg aactgtttat gccgtcacag tatttatgct cttttcgtta
     5461 caaaaaatat attgacgatg cgcctccgta cttgaaaaag gaaatcttaa cggcctataa
     5521 agtgcacaca ggcacaatcg gctatatcgg atggaagccg ataagcaaat atccggcatc
     5581 gggctattgg agctatgaag acaattggat gtggtttggt ccgcacgtct gctggataca
     5641 ggagagtttt tcggcaagta atccgactgc aaagtttatt gtcacagcca tcccttcggg
     5701 ccagcggatc gcccagatag tcgatacgtt cagttgggac agatccctaa attataatgg
     5761 tgctttatca gcgatttcgg ggcaagccaa tatgtgcaac gtcagccgga cattttatga
     5821 tgtgattccg acggccgacc aacttacggg cggctatgtt aaatcctgga actttgttta
     5881 tagcgattca ggcgtgaatg ccaacactgt caatagcgac gtgagtggga tggggacgct
     5941 ggttgaacag ataacggaaa cgcaatatgc cacctatacc ccttgcgggg gggttaaaat
     6001 ggcccgcaat acaacctctc cctaccccgc ccatcccgcc gtcattgggg atgaagtgtt
     6061 tgtttccgtg gcgtgtgatt caacgatgta ttatgaaggt ggttctatgt atcgtggccg
     6121 tgggtggtgc actgaaaacc cgttttcggc ctttgccaaa atgaatctta caacaggcca
     6181 ggtatcctgg tttggctcgt ggtatggggg acaactcggt tccagcgtac caacgcgatg
     6241 ggtgatgcgt attttaggga tagactcacc cagtgtcgtc tttgtcgata actataacta
     6301 tctggtccgc aaagacgatg ctgattatct ggattggctt gaaaaaggaa aatacgtatg
     6361 gcgggtatct tcttcgggtc tgaatttggg atgttatgcc tctatgggcg gttttttata
     6421 tacgggcaat ccttcgagta atacagcatc taagattgat ttatcaaacg gcagttttat
     6481 ggacatcaca agtgccatcg gcggtgcaat tattgggcgc tatggggatt atttaagggt
     6541 acgaaataat gcatacatgg accaggaata tctggttgat ccgaacaatt ttgccaataa
     6601 acgagcgata cagccggccg gcgtgcttct gtatccttcc tgcaatcacg ctgaatgtgt
     6661 gtcgtgggga tatgaccggc tcaacaccgt actacgagat tcgggagact ctcgtgttgg
     6721 aatgggcgat ctgcacgttt atgatgattg ggatatgggg tcgttttcca gtgttctgga
     6781 cttaacagcg gtagaatttt aatgtcctgt tgtggaaaaa ccattcggaa ggtcatccat
     6841 atcgccaaag ggacgatggc agatatgctt ggtatcaagt atgagtttac ggatgggagg
     6901 gttcggacct gtcggaactg tgaaaatcgt acgtggctga gtgcttcaga gtattgggag
     6961 tgggtggccg accatggtgg acgaatgaag tttatggcgg agttagacga tttatcgagt
     7021 tggccaccac tgccgaaagc tgaatatcag gtcggcaatc tgctgtattg tcgaatctgc
     7081 aagtgccgga ttacccggaa ggcaaggatt gaggcagaaa agtgtcccca aggcaattgg
     7141 tgattgacaa gtcggggtgg gttttgttac aataacgccg ttggaatgct ccttctgccg
     7201 cctgcggata gccctgggaa gttgcaccaa tttcctcctc cttttgccgc aggcggtttt
     7261 ttgtctccat ggcaagaaca tctgttcaat agtccccata gggagaagta cagattgaca
     7321 atacggggaa atacccaaat ctcaacggag ggcgccattt tagcagtttt ctgctaaaat
     7381 taaacacaac agctacttgt ttcattcctt aaaaaccgct actatagcca acgaatgtca
     7441 aattgttacg gattaaaaag gagcttgtat gattagattt gactgcagca aatgtgagca
     7501 taactaccgc gtcccagatg aatacgccgg caaacgaatc cggtgcaagg catgtgatac
     7561 ggttattact attcctgccc ctgaaactga aacggtgaag tgtggcgata gtattgccac
     7621 ctacaatcgt ctgcttgaag agatgtcaaa gtacgaaaag caagcaccgg cgattgatag
     7681 tgaatcctga tgtaaagtaa taatgtccgt ccgctccttc tgccgcctgt ggagcttaaa
     7741 aagaagcatc acctgcttct tcctccttct ctccacgggc ggtttttttg tttaaatgac
     7801 catgggataa cgtacactat ctttcgcaca ttattatcgg gccaaaaaga aaagccctcg
     7861 ctctggtcga tggaagagtt tagaggaaca aaaccatttg gcagagaaag ggcctgttat
     7921 gagcgacaaa gaagcatcgg ttaacgtggg aaaaaacttg aatggcgtga tcaaaattga
     7981 cgcggcccag attcattcgc acctggacag catggttcgc agcacggttg aggagacgct
     8041 caacaagctg ctggacgagg aggcggaccg gctgtgcaat gcgggtcgat atgagcacag
     8101 cgacagccgc accgacacgc gggcgggcca ttatccgagg acctatcatg ccaaggtcgg
     8161 tccgctgact ctgaagattc ccaagctgcg caagacgacg tttgagacgg cgattatcga
     8221 gcggtatcgc cgtcgggagg cctcggtgga agaggctctg attgagatgt atctggccgg
     8281 ggtttcggtc cggcggatcg aggacatcac cgaggcgtta tggggcacca aggtcagccc
     8341 cggcacggtc agtgaactca acaagaagat ttacagccag atcgagctgt ggaggaatcg
     8401 cccgcttgaa ggctcttacg cctatgtgta cctggacggg atttatttga agcgcagctg
     8461 gggcggcgag gttcgcaata ttgccgtcct ggtggcggtc ggggtggatt cactgggttt
     8521 tcgtgagata ttgggcgttt gcgaggggat caaggaagac caggagagct ggcggagttt
     8581 cctgcggcac ttgaaacagc ggggtctgac gggcgttcag ttggtgattt ctgataaatg
     8641 cctgggactg gttgagacgt tgggcgaatt ctttgccgat gcccgatggc agcggtgtac
     8701 ggtgcatttt taccgcaacg tgtttagctg cgttcccaac gacaaggtca aggcggtcgc
     8761 cgggatgctc aaggcgatcc acgcccagga gagccgtgac gcggcgttgc agaaggtggc
     8821 agcggtcgtc gagaggctgg ttgcgatgaa gctatccaag gcggccgaaa cgctccgtgc
     8881 aggcatcgct gaaacgctgt cgtattatga tttcccgcgg gagcattgga ttcggattga
     8941 ctgtccccct gggttagggc catttcgtgt gttaggattt ccgacattga tt
//
LOCUS       DAOTBO010000107         8340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7289_length_8340_cov_4.704888, whole genome shotgun sequence.
ACCESSION   DAOTBO010000107 DAOTBO010000000
VERSION     DAOTBO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7289_length_8340_cov_4.704888"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLE88_10675"
     CDS             complement(<1..552)
                     /locus_tag="PLE88_10675"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HOM76970.1"
                     /translation="MARKIPQISVIQVAARGITATRFSMALKKPEQVTVRRIAGSWEG
                     EAYEEAFRTFINELKIQEDRVVLVLPRQDVTTRLLTLPSHSLEEVAGMVQFSAEEYVP
                     YTLDELVIDQCIIKTLDSGESEVMAAFPHKDVVNRPLSLLQQCGITPEKILLSTACLA
                     LTAVVDPLLSKERHALVSLMDGGM"
     gene            complement(542..1492)
                     /locus_tag="PLE88_10680"
     CDS             complement(542..1492)
                     /locus_tag="PLE88_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOM76971.1"
                     /translation="MPNNGMHKTGFVLISVLWVTAMLTVIALGFGRRAALERRASAYA
                     LDQAEAMLMARGAVERGIIELYNQRYMRMLLPEGQRGGDHCGQSWAKKKNLYTEGYFE
                     KRENFDNDEAAYIIIDEDRYINVNAADEKILEEVKSLKRPVIRAINARLNRAGGRGNE
                     EYQPFNAVEEIRYLRGVTEDNWFGSKNAPGLKNLLTVWGTAQINVNTASEEVLSCIPG
                     LKKTEINAILRYRAGSDGVLYTDDDQGFEDMNDLLTKAGLSESGSADWGAYCKFNASH
                     FRITGVATRRQGKVRAICSAIFAAGNYIQVLDWQEKTLGS"
     gene            complement(1482..2750)
                     /locus_tag="PLE88_10685"
     CDS             complement(1482..2750)
                     /locus_tag="PLE88_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOM76972.1"
                     /translation="MKCRLFISVNLLCCLALIGCKAPQKVEAPQYDKPLPPGQYALRK
                     ITDPSMIPDMTFACYNLKDVREGIQNSLSYLSKPSSKGFFPMADITHDRAVASLRAMA
                     ELLDSGLYGKDLHAAIRSKFEVYQSVGCDDKGTVLFTGYYTPIFDGSLTPSDKYRYPL
                     YKQPADLVKNEKGDILGQRMPDGSLRKYPSRKEIEQSGMLKGQEIAWMADPFEVYIAH
                     VQGSALLRTPDGKMVTLGYAASNGHEYNSIVGRAEHLFEGDNKSITMFVVAQPMDLDE
                     GEGSRLMRVTYSYNRSKKTIEREEALVTGALPTRPPQDEQLDRSRIKLQRRYKSTVAE
                     NVGQFEITYIWVPLSDQITPGLPPEPEPPIYKKESRERWGLPQGIRLEVEMRDPDNKE
                     KTYQFNTVLPMRAQSARQKRDRLEEMMSAK"
     gene            2959..3597
                     /locus_tag="PLE88_10690"
     CDS             2959..3597
                     /locus_tag="PLE88_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOM76973.1"
                     /translation="MKTLLKVSACLSIVSLLGCTSGSSYSRKGFDFSQIDKVAVVDVI
                     GPVGGEAAKNQIADFFMMELLKKGYSPIERSQVQILLNEQKFQASDITSPEGAARAGR
                     ILNVPVIMYINVPKFGENISMTVKMVNVEDGSILWMGEGTGTGGKTLGTILGAAAGAA
                     VGAAATGSDDRVVGAVIGGVAGGGAGYLLTPQTEEKVRKMAEKICKTMPVRQ"
     gene            3629..4957
                     /locus_tag="PLE88_10695"
     CDS             3629..4957
                     /locus_tag="PLE88_10695"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOM76974.1"
                     /translation="MAQTRTVKIGMVGYGTVGTGVAKVLFENADEITARTGLKLELAC
                     VVDKDTQSARKFQIPQKVLTDNLDALLNDPSIEIGLELVGGTTAAKDVQLKLLKAGKH
                     VVTANKALLAEHGPQLYQAALEAGRCIAFEASCCGGIPIISALRTGLAANRITGLYGI
                     FNGTCNYILTNMTRRNMPFAEALKEAQARGFAEQDPSLDISGIDTAHKLAILAGLAFH
                     CPIRMSDICIEGIEHIDSVDIRSALEMGYILKLLAIGEKDSEGRISLRVHPSFISENT
                     PLARVDGPFNAVSVFGNAVGNTMFYGRGAGMMATASAVVADIIDIAIGNSWRLFETMP
                     MAVHSHSRAHIKSIEDIDSRFYLRIMAKDEPGVFAQYGQTLGKHRISISGAIQHEWTG
                     PDNTVPVVITTHPTAQRNMNAALKELEQLDIIRQKPVCIRIVDIPEDKEP"
     gene            4960..6147
                     /locus_tag="PLE88_10700"
     CDS             4960..6147
                     /locus_tag="PLE88_10700"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOM76975.1"
                     /translation="MKYVIIVPDGAADNPQACFGGKTVLEAAETPNMDKIAMDGRQGI
                     CRTVPAAMTPGSDVAMMSLLGYDPAKYYTGRAPIEAVAQNIPLGPDDWVFRCNLATTA
                     DNKMMDHSAGHISTKEASVLINELDKAISLDSVAFYTGVSYRHLCVIKGQDFSKVVTQ
                     PPHDFIGEKLSKILPRGKNSEFLNTLIAKSVQLFENHPINKVRRDLGENPVTTIWLWG
                     QGQRASMERFEKKYGKKGAAITAVDLVRGLAKLIGFDLINVAGATGYLDTNFAGKGEA
                     AIEALDKYDIVLVHIEAPDEAGHSGNPYHKKQAIESIDKFIVGPVYEALQTYSQYRIL
                     VMPDHPTPVEERCHIGEPVPFALAGYGIKGILKKPFCERNSFESGFEIKNGPDLMEYL
                     IKV"
     gene            6257..7453
                     /locus_tag="PLE88_10705"
     CDS             6257..7453
                     /locus_tag="PLE88_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76976.1"
                     /translation="MSRNWRNRLIIIFCLSSILSADTFRHRSQNIVYTGYATHKTNNG
                     LNVVNTLEKGQLELNLADYQIEFNEQGRNPFVPVLAIDDEIAYEYAVQSFESAIIEES
                     DKGPLMILIELDTPGGRVDLAKRICAAITGIKYCKTVCYVKGGKHGGAFSAGAAVALA
                     CDTLYMAPETSIGAAAMIATTEAGLTMDMKQAFGETVGEKYNAAWRNYLASLAQENNR
                     PGILAKAMADMDIAVVEVKRNGQQLFIDPQEQRANDAFVRTISRKGELLVLTAADAAA
                     LHIADGVADSLASLISRLGYPQARIESNKRLIAAKEDFEKALRRFDQLKEKLDLKFKE
                     ITAKSQRSALTRSQAVHDLDELARNAEYLLRLKRSYPDIPVSEEAILQLVNNIKAESK
                     SIKAMR"
     gene            7477..>8340
                     /locus_tag="PLE88_10710"
     CDS             7477..>8340
                     /locus_tag="PLE88_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76977.1"
                     /translation="MKNAVRLSRWLLICILASAAPADTFKNRQTGETFTGFVTQKTAG
                     SKTLIFNSEQNKMIPVVLSDYEITYNIQGRRDSVVVIPITQPEILLSQTLSEKVAAAI
                     VDASNTGPQAIILQIDNPGGRGDYMALIASALSQTANCPTAAFISGGAHGGAFAAAGV
                     VAAACQKVYIAPNASIGAVGPTTGIAASTLSFGDFLSTYCPDTLVTYSTYVSTLAQQR
                     GRPDLLVRALVDKRLSIAEVMNINGSRQFIQKNDRQPTQTLVRTLAEGLSGTAADTSK
                     LTPSDLAGAVLT"
BASE COUNT         2142 a         2080 c         2105 g         2013 t
ORIGIN      
        1 catgccgccg tccatgagag aaacgagcgc atgccgctcc ttcgataaaa gaggatctac
       61 aacagctgta agcgcaaggc aggctgtgct cagcaggatc ttctccggtg taatgccgca
      121 ttgttgcaga agggatagcg gtctattgac aacatctttg tgaggaaagg ccgccattac
      181 ttccgattca ccggaatcga gcgttttgat aatgcactgg tcaatgacca gttcatccag
      241 cgtgtaaggc acatattctt cagcgctgaa ttgcaccata cccgccactt cttccaggct
      301 gtgggagggc agggtcagca ggcgggtcgt tacatcctgc cggggcagta ccagcaccac
      361 cctgtcttcc tgtattttca gctcattaat gaaagtgcgg aatgcctctt catacgcctc
      421 gccttcccag ctgccggcaa tacgccggac tgtcacctgt tccggctttt ttaaggccat
      481 ggaaaagcgt gtcgccgtta ttccccgggc ggcgacctga ataacactga tctgtggaat
      541 tttacgagcc aagcgttttc tcctgccagt caagaacctg tatataattg ccagccgcaa
      601 agattgcgga gcaaatagcg cgcaccttgc cttggcgacg cgttgccacg cctgttatcc
      661 taaaatgtga agcgttgaat ttgcaatagg cgccccagtc agcggagccc gattctgaca
      721 gacctgcttt ggtcagcaga tcattcatat cctcaaaacc ctgatcatcg tcggtatata
      781 atacaccatc cgacccggcg cgatacctca gaatggcatt aatttcggtt tttttcagcc
      841 cgggtatgca ggacagcacc tcttcggagg cggtattaac gttgatttgc gcggttcccc
      901 agacagtgag caggtttttc agacccggcg cattctttga gccgaaccag ttgtcttccg
      961 tcacgccccg cagatagcgg atttcctcca cggcattgaa aggctgatat tcttcattgc
     1021 cccggccgcc cgcacggttg agccgggcat taatggcacg aattaccggc cgcttcagag
     1081 atttcacttc ctccagaatc ttttcatcgg ccgcattgac attaatgtag cggtcttcat
     1141 cgataatgat ataggcggct tcgtcgttat caaaattctc ccgtttctcg aaataacctt
     1201 ctgtataaag attctttttc tttgcccagg actgaccgca atggtcaccg ccccgctgac
     1261 cttccggaag cagcattctc atgtaccgct ggttgtacaa ttcaataatg ccacgttcca
     1321 cagcgccccg tgccatgagc atggcttctg cctgatccag cgcataggcg gaagcacgtc
     1381 gttccagagc ggcacgtctg ccgaagccca gtgcgatcac ggtcagcatt gccgtgaccc
     1441 acaacacgct tatcagcacg aatcctgttt tatgcatccc gttatttggc actcatcatt
     1501 tcctccaggc gatcccgttt ctggcgggca ctctgagcac gcatgggcag tactgtgttg
     1561 aattgatacg ttttctcctt gttgtccgga tcccgcattt ccacttccag cctgatgccc
     1621 tgaggcagcc cccaccgctc tcttgattct ttcttgtaaa tcggcggttc cggttcggga
     1681 ggcagaccgg gggtgatttg atcggataaa ggcacccaga tataggttat ttcgaactgt
     1741 ccgacatttt cagccactgt agatttgtag cgacgctgca gcttgattct ggatctgtcc
     1801 aattgctcgt cctgaggcgg tcttgtcgga agtgcgcctg tcacgagcgc ctcttcccgc
     1861 tcaatagtct ttttgctgcg gttgtatgaa taagtgaccc gcatgagccg ggagccttcc
     1921 ccttcatcca gatccatagg ctgggcaaca acaaacatgg taatggattt gttgtctcct
     1981 tcaaagaggt gttccgcccg gcccacgatg ctgttgtatt catggccgtt gctggccgca
     2041 tagcccaagg ttaccatttt gccgtcaggg gtacgaagca gcgcagaccc ttgaacatga
     2101 gcaatataga cctcaaacgg atccgccatc caagcaatct cctgcccttt gagcatccca
     2161 gattgctcaa tttctttacg ggagggatat ttccgcagtg aaccatccgg catgcgctga
     2221 ccaaggatat cgcccttttc attcttgacc agatcggccg gctgtttgta gagcggataa
     2281 cggtatttat ccgatggtgt cagagaccca tcaaaaatgg gggtataata accggtaaac
     2341 agcaccgttc ccttgtcatc acatcctacg gattgataca cttcaaattt gctgcggata
     2401 gccgcatgca gatccttccc gtaaagaccg ctgtccagca gttcggccat cgccctcagc
     2461 gaagctacgg cgcggtcgtg tgtgatgtcg gccataggga aaaagccctt gctggagggt
     2521 ttcgacaaat aactaaggga attctgaatg ccttcccgca catccttgag attgtagcag
     2581 gcaaacgtca tatccggaat catcgacggg tcggtaatct ttcgaagagc atactgtccg
     2641 gggggcaagg gcttgtcata ctggggggcc tcaacttttt ggggggcttt gcacccaatc
     2701 agagcaagac agcacagcaa attgacactg ataaaaagtc tgcatttcat agaatctccc
     2761 tgcattttaa aaatccgttt tggctttcgg gtcaaggtaa aaatacctac attatgggta
     2821 ttccgtctgc tttgtcaaat attttacaca tcgaactttt agaggcagtt tctttaataa
     2881 agaaaagcgt tgacaattta acacacacac ctgaaaatat tgcgtaaatc ggaaattaag
     2941 gtgatttagg agcatattat gaaaacacta ttgaaggttt cggcgtgttt gagtatcgta
     3001 tcactgcttg gctgtacatc cggttcaagc tattcccgca aaggctttga cttttctcag
     3061 attgacaagg tggcagtggt ggatgtcatc gggcccgtag gcggtgaagc ggccaaaaac
     3121 cagattgccg acttttttat gatggaactg cttaaaaaag gatattctcc aattgaacgt
     3181 tcccaagtcc agatactgct caatgaacag aagtttcagg cttccgatat aaccagtccg
     3241 gaaggtgcag ccagagccgg ccggattctc aatgttcctg tcattatgta catcaatgtc
     3301 cccaagttcg gggagaatat ttcgatgaca gtcaaaatgg tcaacgttga agacggcagc
     3361 attttgtgga tgggagaagg cacaggaacc ggcggcaaga cactcgggac gattctgggt
     3421 gctgcggctg gtgcggctgt cggagcggcg gccaccggca gcgatgaccg cgttgtgggt
     3481 gctgttatcg gaggcgttgc cggcggcggc gccggctatc tgctgacacc ccaaaccgaa
     3541 gaaaaagtgc ggaaaatggc cgaaaaaatc tgtaaaacca tgcccgttcg ccagtaatgg
     3601 tgttgtaaat taaaactacc cccttctgat ggcacagaca agaacagtca aaatcggcat
     3661 ggttggatac ggcaccgtcg gtacgggtgt tgccaaagtt ttgttcgaaa atgccgatga
     3721 aattacagcg cggaccggct tgaaactgga gctggcctgt gtggtggata aagataccca
     3781 gtctgcacgg aaatttcaaa tcccgcaaaa ggtgctgacc gataatctgg acgccctgct
     3841 caatgatcct tcgatcgaga tcgggctgga actggttggc ggtacaacag cggccaaaga
     3901 tgtgcagctg aaactgctga aagccggcaa acatgtggtt actgccaaca aggcgcttct
     3961 ggccgaacat gggccgcagc tgtatcaggc agcgctggaa gcaggccgat gcatcgcttt
     4021 cgaggcaagc tgctgcgggg gaatcccgat tatttccgcg cttcgaacgg gtctggcagc
     4081 caaccgcatt accggacttt acggcatctt taacggcacc tgcaactaca tcctgacaaa
     4141 tatgacccgc cggaatatgc cgtttgcaga agccctcaaa gaggcccagg cacgcggatt
     4201 tgccgaacag gatccttctt tagatatcag cggcatagat acagcccaca aactggctat
     4261 tttggccggt ctggcatttc actgccctat ccgtatgagt gacatctgca tagaaggcat
     4321 cgaacatatc gacagcgtcg atattcgaag tgctctggaa atgggatata tcctcaagct
     4381 gctggctatt ggcgaaaaag attccgaagg cagaatatcg ctgcgggtcc atccctcttt
     4441 tatatctgaa aatacccctt tggcccgtgt ggatgggcct ttcaatgctg tcagcgtttt
     4501 cggcaacgca gtcggcaata caatgtttta cggccggggc gccgggatga tggcaacagc
     4561 cagtgcggtc gtggcagata tcatcgatat cgccatcggc aacagctggc gattgtttga
     4621 aacaatgccg atggcagtcc actctcacag ccgcgcgcac attaagtcaa ttgaagatat
     4681 tgacagccgg ttttatcttc ggataatggc caaagatgaa cccggcgtgt ttgcccagta
     4741 cggccagacg ctcggcaagc accgcatcag catcagcgga gcaattcagc atgagtggac
     4801 agggccggat aataccgtac cggtggtcat tacaactcac ccaacagctc aaagaaatat
     4861 gaatgctgca ttaaaggagc tggagcagct ggatattatc cggcagaaac cggtttgcat
     4921 tcgtattgtt gatattccag aggacaaaga accgtgacaa tgaaatatgt gattattgta
     4981 cctgacggag cagctgataa tccgcaagcc tgtttcggcg gcaaaactgt tcttgaagcc
     5041 gccgaaaccc cgaatatgga caaaattgcc atggacggcc ggcagggcat ttgccgcact
     5101 gttccagccg ctatgacccc cggcagcgat gtggccatga tgtcgcttct gggatatgat
     5161 cctgccaagt attacacagg ccgtgcccct atcgaggccg tagcccaaaa tatcccgctg
     5221 ggtcctgatg attgggtttt tcgatgcaat ttggcgacaa cggcagacaa taaaatgatg
     5281 gaccatagtg ccggccacat ttctacaaaa gaagccagtg ttttaattaa tgagttagat
     5341 aaggcgattt ccttagactc cgttgccttt tatacaggcg tcagctatcg tcatttgtgt
     5401 gtcattaaag ggcaggattt ttcaaaagtg gtaacgcagc cgccgcatga ctttattgga
     5461 gagaaattgt caaaaatcct tcccagaggc aaaaactccg aatttctcaa tacactcata
     5521 gccaaatccg tacagctttt tgaaaatcac cccatcaaca aggtccgtcg ggatttaggt
     5581 gagaatcccg taacaacaat ctggctttgg gggcagggac agcgagcctc gatggagcga
     5641 tttgaaaaaa aatacggcaa aaaaggagct gctattactg ctgttgattt agtgcggggt
     5701 ttagccaaac tcattggatt cgacttaatc aatgttgcgg gggcaactgg atatttagac
     5761 accaattttg cgggcaaagg cgaggcagct atcgaagcgc ttgacaaata cgatattgtt
     5821 ctggttcata tcgaggcgcc ggatgaggca gggcattcgg gaaatccata ccacaaaaaa
     5881 caggcgatag aatctatcga caaatttatt gtaggtcctg tttacgaagc actccaaaca
     5941 tacagccaat accgaatcct cgtcatgcca gaccacccga ctccggtaga ggaacgctgc
     6001 catattggtg aacctgtacc ttttgctttg gccggctatg gaatcaaggg tattctcaaa
     6061 aagcccttct gcgaacgtaa cagttttgaa tcgggctttg aaatcaaaaa cggccctgat
     6121 ttaatggaat accttatcaa ggtataaccg actacagaaa aacagggcaa aagatgtcca
     6181 aaggataatg ttttaactct ggtttgacca tttcgttggc tttcagcgta taacatttaa
     6241 atacgcggga aaaaagatga gcagaaactg gcgcaataga ctaattatta tcttttgttt
     6301 gagcagcata ctttctgccg acacttttcg tcatcgaagc cagaacattg tttataccgg
     6361 ctacgcgaca cataagacaa acaatggcct caatgtcgta aacacccttg aaaaaggaca
     6421 gctcgaattg aatttggctg attatcagat cgaatttaat gaacagggaa gaaatccctt
     6481 tgtgcccgtt ctcgcaatag acgatgagat tgcctatgaa tacgctgtac aatcttttga
     6541 aagtgctatc atcgaagagt ccgataaagg gcctctgatg attttaattg agctggatac
     6601 cccgggggga cgggtagatt tagccaaacg catctgtgca gcaattaccg gcatcaaata
     6661 ttgcaaaact gtctgttatg taaaaggggg caaacatgga ggggcctttt cggctggggc
     6721 agcagtagca ttggcctgcg atactttgta tatggcacct gaaacatcca tcggggcggc
     6781 tgctatgatt gccaccacag aagccggcct gacaatggat atgaaacagg ctttcgggga
     6841 gacggtagga gaaaaataca acgccgcgtg gcgcaattat ctggcctctt tggcacagga
     6901 aaacaaccgc cccgggatat tggccaaagc aatggcggat atggatatcg ctgtggtaga
     6961 agtgaagcga aacggccagc agctgtttat agatccgcag gaacagcgag ccaatgatgc
     7021 ttttgtacgg acgatttcac gcaagggtga actcttggtc ttgacggcgg ctgatgcggc
     7081 ggccctgcat atcgctgacg gtgtggctga ttcgcttgcg tctctgatca gccggttagg
     7141 gtatcctcag gccagaattg aaagcaataa acgtctgatt gccgccaagg aagattttga
     7201 aaaggcgctt cgccgatttg accagctcaa agagaaattg gatctgaagt tcaaggagat
     7261 aactgccaaa agccagcgca gcgccctgac gcggagccag gccgttcacg acttggacga
     7321 attggcccgc aatgcagaat atcttcttcg actcaaacgc agctacccgg atattcctgt
     7381 ctcggaagaa gcgattcttc agcttgtcaa caatataaag gcagaatcaa aatcgattaa
     7441 ggcaatgcga taaacaagcc aaatgggagg tgctttatga aaaatgctgt acgattaagc
     7501 cgatggctgc tgatatgcat tctggcttcc gcggcaccag cagatacatt caaaaacagg
     7561 caaaccggtg agacttttac cggatttgtt acgcaaaaga cagcgggcag caaaaccctg
     7621 attttcaaca gcgaacaaaa taagatgatt ccagttgtct tgagcgatta cgaaattact
     7681 tacaatatac agggccgtcg ggacagtgtg gtggtgatac ctattacgca gccggaaata
     7741 ctgctttcgc aaacactttc ggaaaaagtt gccgccgcaa tcgttgatgc ttccaataca
     7801 ggccctcagg cgattatact gcaaatagac aatcccggcg ggcgcgggga ttatatggct
     7861 ctcattgctt cggctctttc gcagacggcc aattgcccga cggcggcatt tatctcagga
     7921 ggggctcacg gcggagcatt tgcagccgcc ggcgtggtgg cagcagcctg tcagaaagtc
     7981 tatatcgccc ccaatgcctc gataggagct gtaggaccaa ccacaggaat agccgcaagc
     8041 acattgagtt ttggggattt cctttcaaca tactgccctg acactcttgt cacatacagt
     8101 acgtatgtgt ccacactggc ccagcagcga ggccgtcccg acctgcttgt gcgggcgctg
     8161 gtggacaaac ggctgtctat tgcagaggtg atgaacatta acggcagccg gcaatttatt
     8221 cagaaaaatg accggcagcc gacacaaacc cttgtccgca cacttgccga aggactttca
     8281 ggaacagctg cggatacatc aaaacttacg ccttccgatc tggccggagc cgttttgaca
//
LOCUS       DAOTBO010000108         8161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7484_length_8161_cov_5.422897, whole genome shotgun sequence.
ACCESSION   DAOTBO010000108 DAOTBO010000000
VERSION     DAOTBO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7484_length_8161_cov_5.422897"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLE88_10715"
     CDS             <1..238
                     /locus_tag="PLE88_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76978.1"
                     /translation="PRDKRMELLDLFERCNVAAYLGGHMHKLTMNRYKQMQLVNAEAL
                     SKNFDGRPLSFRIWYVQPPLPPRSEFIALDKNNP"
     gene            400..2322
                     /gene="thrS"
                     /locus_tag="PLE88_10720"
     CDS             400..2322
                     /gene="thrS"
                     /locus_tag="PLE88_10720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOM76979.1"
                     /translation="MAKVKLPDGSTLDVADGTPVSEAVARIGSGLARAAVAAKLNNHL
                     VDLSCPIAGDSDLVVITQKDKEGLDIMRHSCAHIMAEAICSLWPAAKLVYGPNVADGF
                     YYDIDLDTPIRPEDFERIEQKMADIIKSDARFVRIEMSREEAIRKMAGDQYKLDNIQN
                     AKGDTISFYRQGDGFEDLCRGPHIPRTGVLDPKAFKIMSVAGAYWHGDASQKMLQRVY
                     GTCWRNKKELDDYLHQLEEAKKRDHRLIGRQMDLFSFQDEGPGFAFLHPKGMRIWNEI
                     VDYWRAVHRRYGYEEVKTPIMLNESLWHRSGHWDNYKENMYFTTVDNITYAVKPMNCP
                     GGLLIYKTSKHSYRELPMRVAELGLVHRYEASGQMHGLVRVRQFTQDDAHIFCTPEQI
                     ESEIVGVINLIQEIYAAFGFADYHVELSTKPEKHIGSDEIWETATNALKAALDHKKMQ
                     YKINEGDGAFYGPKIDFHINDCLKRSWQLGTIQLDFSMPQRFELVYTAADNTEQTPVM
                     IHRAILGSLERFLGILIEHYAGHFPLWLAPEQVRVLPISEKTNDYAAAVLAQLKQAGL
                     RAWADISDDKIGAKIAKAHTDKVPYMLVVGPKEAQAGLVNVRSWPSQQTQTVSIAQFI
                     STAQQKIADKSLELAL"
     gene            2365..2904
                     /gene="infC"
                     /locus_tag="PLE88_10725"
     CDS             2365..2904
                     /gene="infC"
                     /locus_tag="PLE88_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOM76980.1"
                     /translation="MFLERYRAINKDSLRINDRIRTSPVRLIDENNVQVGIVSIKEAL
                     ERAQEAGLDLVEVAPNSTPPVCRIMDFGKYIYEQKRKIKLSQKKQHTVVLKEIRLRPK
                     IDDHDRDIKIGHAAKFLEKGNKVQFTILFRGREMMHVEQGYELMDQIVQMLQEHGKLE
                     RKAQMLGRRMTMVMAPAKG"
     gene            3119..4891
                     /locus_tag="PLE88_10730"
     CDS             3119..4891
                     /locus_tag="PLE88_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76981.1"
                     /translation="MGKMKMHVRLAGWSLLLCSTLLFAASVEEDWNDFLHYTAIGRFE
                     LAKGYAEQLIQNQADPNVLLTLAEENPEGYRLLLKMHADSDELRQVSGQILALIEQGR
                     YIRRTDPKIIVQEIARLSTTIRGRIAAEERLKNAGEYAIPYMLAALADESRRSEFAYI
                     TEALPKIGRPAIRPLTAALQTDNIALKIEIIRALGKIGYFEPLPYLKLVVEKDSSDLL
                     KAQAVKAIEQIDAGALKIPAAELFFKLGENYYNGTDSLMPAAEFDFANIWFWDAAKQS
                     LIRYEVNKAYFDELMAMRCCEWTLKADPSVGKAIGLWIASFFRAESVGVPMPEYFDAG
                     HADAMTYATTAGPEYLHQALERAIGDNDAYVALGVVEALAVNAGEKSLLYQVGTGQPL
                     AKALSFGDRKVRYSAAIAFAEANPVTEFVGSSAIVENLAAAVVQEGADQIGAEAAGEY
                     ALRSVKAMYKLALVRNKIVDLSKTLPALIQVTQNADPQMRVLAAGVLAHLTSPQAQRA
                     IAAMALSEQNDKEIRISAFESLALSAKLNANLLLTEQIDALYAIVSSTQADPDLRAAA
                     AGAYGALNLPSEQVKRLILDQAKS"
     gene            4979..5245
                     /locus_tag="PLE88_10735"
     CDS             4979..5245
                     /locus_tag="PLE88_10735"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOM76982.1"
                     /translation="MAGNRKKRFENPDFYRGAMRWMGVGIEFCLVIGIFCLVGYYLDT
                     LEGTSPGWLILGFFVGFGVMLYLMLKRAKKDEDERSKEQSDQSE"
     gene            5208..5720
                     /locus_tag="PLE88_10740"
     CDS             5208..5720
                     /locus_tag="PLE88_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76983.1"
                     /translation="MNGLKNSPISRNDPLRRAKRSIDPAPQFPFGRLVFFTGLLAVGG
                     MFIYRLSETGGQMSQTACLSAAAVSLLSALVGIIPVVKTWRRELLWVLLGFFLSGIIR
                     LLIGTLGVVIIILFTKIQRIQFIGYLALFYTAFLAADTWLALWILQNSRIQKQETAVY
                     GTIWDIIGKS"
     gene            5686..6486
                     /gene="atpB"
                     /locus_tag="PLE88_10745"
     CDS             5686..6486
                     /gene="atpB"
                     /locus_tag="PLE88_10745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOM76984.1"
                     /translation="MELFGILLANLNPLKEVVSKPIYEFTVGRWTVAFTNHMFMIALA
                     SAALMIGLPLAVRQKGLVPRGFKNVIETICVFLREDMARPMLKEHTDRYISVLWTMFF
                     FILTMNLLGLTPLDRFVWLFTKKQNWGGAATANIYVTGALATFAFLLFHAAGIRQKGF
                     IKYLATLSPKVPWLMMPFMFVMELLSSFVRLFSLAIRLFANILAGHILLAVLLGFIIM
                     FKNFMVASASILATTLMSLLELFVAFLQAYIFVFLTTVFIMFAVSEEH"
     gene            6569..6826
                     /gene="atpE"
                     /locus_tag="PLE88_10750"
     CDS             6569..6826
                     /gene="atpE"
                     /locus_tag="PLE88_10750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOM76985.1"
                     /translation="MAPMLLAEIVLDDKAFGLLGGLLGTGLIVLGAARGIGQLAGSAV
                     EAIARQPEAGNRIFTTMIIAAALIEGVTLFALIICLLAVLG"
     gene            6850..7446
                     /locus_tag="PLE88_10755"
     CDS             6850..7446
                     /locus_tag="PLE88_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOM76986.1"
                     /translation="MQVRRIVAVLVLLVCICGRLAASEGTAESEAAPSVFEGYFGESI
                     WTLVWFGVLLGVLWKFAWKPLLAGLTGRQEYIERQISDAEKTRAEAKKVLEEYGAKLA
                     DAERQGRDIIAARIRDAEKQAQEVQLKIQQEIEQRKARAEADLDRERIEAEEKLWVQA
                     GEIIRRLGQDVFGKALDDEDNQRLIRQAIDRLKEVSRR"
     gene            7459..8052
                     /gene="atpH"
                     /locus_tag="PLE88_10760"
     CDS             7459..8052
                     /gene="atpH"
                     /locus_tag="PLE88_10760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOM76987.1"
                     /translation="MARSARHIIHEIYSDVMFELAEDAGLLEEVMDDLEAVSKVLQDE
                     PDFLTLLTLGQIKEDEKAAMIRRVFKGRIHDLTLDFLCVLARRNRMGFLHGIGDRYQT
                     LLDERKNIHRIEVTLAKEPTEEQLESLKRQIRDAIQAEIKLAVTVDPEIIGGIIIRKG
                     DLVIDNSVRTILNRTVDAIMSRSKAKTTIKSNNESSI"
BASE COUNT         1985 a         1920 c         2311 g         1945 t
ORIGIN      
        1 gccccgcgat aagcgcatgg aactgctgga tctttttgaa cgctgcaatg ttgctgccta
       61 tctcgggggg catatgcaca aactgacaat gaatcggtat aagcaaatgc agctggtcaa
      121 cgccgaagcc cttagcaaaa atttcgacgg ccgtccgctg agtttccgca tatggtatgt
      181 ccagcccccg cttccgcccc gcagcgaatt tattgcttta gataaaaata atccataata
      241 tcctgtcctg tgatggcgat ttttccgatt tagaattgca tacttgacat cagcaggccg
      301 gttttttaga ctatacctgt tgtttttcag ggcgaacggc gccggatagg tgctgaattt
      361 cgcaaaaaat tacaaaattt cgaggaactg gacaatttaa tggcaaaagt caaacttccg
      421 gatggaagca ccttggatgt cgcagacggt acccctgtat cggaggctgt tgcccgtatt
      481 ggcagcggtt tggcgcgggc ggcggttgcg gcaaagctga ataatcatct cgttgacctg
      541 tcttgcccca ttgctgggga ctctgatttg gtcgtcatca cccagaaaga caaagaaggc
      601 cttgacatta tgcggcacag ctgtgcccat attatggccg aagcgatctg ctcgctgtgg
      661 ccggctgcca agctggttta cggccccaat gtggccgacg gcttttacta tgatattgat
      721 cttgatactc ctatccggcc ggaagatttt gagcgcatcg agcaaaaaat ggccgacatt
      781 atcaagtcgg atgcacggtt tgtgcggatt gaaatgagcc gggaggaagc catccgaaag
      841 atggcagggg accaatacaa gcttgataat atccagaatg caaaggggga tacaatcagt
      901 ttttaccggc agggcgacgg atttgaagac ctttgccgcg gcccgcatat cccgcggacg
      961 ggcgtcttag accccaaagc attcaaaatc atgtcggtgg ccggagccta ctggcacggc
     1021 gacgcctcgc aaaaaatgct gcagagggtc tatggcacct gctggcggaa caagaaggaa
     1081 ctggacgatt accttcatca gctcgaagag gccaaaaaac gcgaccatcg gctgatcggc
     1141 cggcagatgg acctgttcag tttccaagat gaaggacccg gctttgcctt tctgcatccc
     1201 aagggcatgc gcatctggaa tgagattgtc gattactgga gagccgtgca ccgccggtac
     1261 ggctatgagg aagttaagac ccccatcatg ctcaacgagt cgctgtggca tcggagcggc
     1321 cattgggaca actacaaaga aaatatgtat ttcaccacag tggacaacat cacctatgct
     1381 gtcaagccga tgaactgtcc ggggggcctg ctgatttaca agaccagcaa gcattcctat
     1441 cgtgaactgc cgatgcgggt agccgagctg gggctggtgc atcggtatga agccagcggg
     1501 cagatgcacg gtctggtgcg ggtccggcag tttacccaag acgatgccca tatcttctgc
     1561 acgcccgagc agattgaaag cgagattgtg ggggttatca acctgattca ggaaatctat
     1621 gcggcgttcg ggtttgccga ttatcatgtg gagctttcca caaagcccga aaagcatatc
     1681 ggttctgatg agatctggga aacggcaacc aacgccctga aagccgctct ggaccataag
     1741 aagatgcagt acaagattaa tgaaggcgac ggggcatttt atgggccgaa aattgatttc
     1801 catatcaacg actgcctgaa gcgttcgtgg cagctgggga ctattcagct tgatttttca
     1861 atgccgcagc gttttgagct ggtctatacg gctgcggaca ataccgaaca gaccccagtg
     1921 atgattcatc gggcgatatt gggttcattg gagcggtttt tgggcatcct gattgagcat
     1981 tatgcggggc atttccccct gtggctggcg cccgaacagg tgcgggtgct gcccatcagc
     2041 gaaaaaacaa atgactacgc cgcggcggtg ctggcgcagc ttaaacaggc cggcctgcgg
     2101 gcttgggcgg atatctccga tgacaaaatc ggggccaaga ttgccaaggc ccacaccgat
     2161 aaggtcccgt atatgctggt tgtcggccct aaagaagcgc aggcaggtct ggtgaatgtc
     2221 cgcagttggc ccagccagca gacccagacc gtctcaatag cccagtttat ttcaacagcc
     2281 cagcagaaaa tagccgataa atctctggag ttggcattgt aaaaggtctg tctggaaacg
     2341 gttccggcag cttattaaag agtcttgttt ttagaaaggt acagagccat taataaagac
     2401 agtttacgga taaatgaccg gatccgaacc agtccggtcc gcctgattga tgaaaataac
     2461 gttcaggtcg gtattgtcag catcaaagag gcattggaaa gggcacagga agctggactg
     2521 gatcttgtgg aggtggcccc caactcaaca ccgccagtat gccgaattat ggatttcggc
     2581 aaatacatct atgaacagaa gcgcaaaatc aagctgagcc agaaaaagca gcacacagtg
     2641 gtcctgaagg aaatccgcct gcgtcccaag attgacgacc acgacagaga tatcaaaatc
     2701 ggccatgccg ctaaatttct cgaaaaagga aacaaagtcc agtttaccat cctcttccgc
     2761 ggccgggaaa tgatgcatgt tgaacagggc tatgaactga tggatcagat tgtccagatg
     2821 cttcaggagc acggcaaact ggagcgcaag gcccaaatgc tcggacgacg gatgacgatg
     2881 gttatggcgc cggccaaggg gtaatcggag ggtctggatg gagcggcttt tctaaaacgc
     2941 cctcttcctt tatccagcag cgaataaagc ggtttttgat tgacagcttg ccggcggttc
     3001 ctataataaa tcagacaccg ttttttcaac gggatgtaag atgcataact tctttttaga
     3061 aagagattga gagtctgtcg gacaaagccg gagcagacag agtaatggga gataatcgat
     3121 gggaaaaatg aagatgcatg tacggctggc agggtggagt ctgctgcttt gttcaacatt
     3181 gttatttgca gcaagcgtag aagaggactg gaacgatttt ctgcactata cggccatcgg
     3241 gcggtttgag ctggccaagg gctatgccga acagctcatc cagaatcagg ccgaccccaa
     3301 tgtcctgctg accctggctg aggagaatcc cgaaggctat cgtctgctgc tgaaaatgca
     3361 tgccgacagc gatgaactgc ggcaggtcag cggccaaatt ttggcactga ttgagcaagg
     3421 ccgctatatc cgccgcaccg atccgaaaat cattgttcag gaaattgccc gtctgagcac
     3481 aaccatccgc ggacggattg ccgccgaaga acggcttaaa aatgctggcg aatatgccat
     3541 tccttatatg ctggcggctc tggcggatga atcccgccgc agcgagtttg cttatattac
     3601 cgaggcgctg ccgaagatcg gccgtccggc gattcggcct ttgacagcag cgcttcagac
     3661 agacaatatc gccctcaaaa ttgaaatcat ccgggcgctg ggcaaaatcg gctattttga
     3721 acctctgccg tatctgaagc tggtggtcga aaaggactcc tccgatctgc tcaaggcgca
     3781 agctgtcaag gctattgagc aaattgatgc gggggctctt aagattcctg cagccgaact
     3841 gttctttaag ctcggggaaa attactataa tggaaccgat tccctcatgc cggcggcgga
     3901 gtttgacttt gccaatatct ggttttggga tgccgccaaa cagtcgctga ttcgctatga
     3961 ggtcaataag gcctattttg atgaactgat ggcgatgcgc tgctgtgaat ggacgctcaa
     4021 ggctgatccg agcgtcggga aagccatcgg cttatggatt gcttcctttt tccgtgcaga
     4081 atccgtcggt gttccgatgc ccgaatactt tgatgccggt catgcggatg caatgaccta
     4141 tgccacaacc gcaggcccgg aatatctgca tcaggcactg gagcgggcca ttggcgataa
     4201 cgatgcctat gtcgctttgg gcgtagttga ggcgctggct gtaaacgccg gcgaaaagtc
     4261 tcttctgtat caggtgggga cagggcagcc tttggccaag gccctcagtt tcggcgaccg
     4321 caaggttcgc tacagtgcag ccattgcttt tgccgaagcc aatccggtaa ccgagtttgt
     4381 cggcagcagc gccattgtag aaaatctggc cgccgctgta gtacaggaag gagctgacca
     4441 aatcggcgca gaagcggccg gagagtatgc cttgcgctcg gtgaaagcaa tgtataagct
     4501 ggccttggtc cggaacaaaa tagtggactt atccaagaca cttcctgctt taatacaggt
     4561 tacgcagaat gcggaccccc aaatgcgggt tctggcagcc ggggttttgg cgcatctgac
     4621 aagccctcag gcccagcggg ctattgcggc aatggcgctg tcggagcaga atgacaagga
     4681 aattcgcatc tcagcgtttg aatctctggc gttatcggcc aagctgaatg ccaacctgct
     4741 gttgaccgaa caaatcgatg ccctgtatgc cattgtcagc tcaacccaag ctgatcctga
     4801 tttgcgtgca gctgcagccg gcgcatacgg cgcattgaac cttcccagtg aacaggtcaa
     4861 gcggctgatt ctcgaccaag ccaagtcttg aatgcactgg atggtttttt cagtctgaac
     4921 cggaaaaacg aaggtttccg gcgatagagc ttgttttttg ctttcagaaa ggtgaaaaat
     4981 ggctgggaac cggaaaaaaa gatttgaaaa tcccgatttt taccgcgggg ctatgcgctg
     5041 gatgggggtg ggtattgaat tctgtctggt catcggcatc ttctgtctgg ttggctatta
     5101 tctggataca ctggaaggaa caagtccggg ctggctgatt ctgggatttt ttgtcggttt
     5161 tggggtgatg ctctatctaa tgttaaagcg agccaaaaaa gacgaggatg aacggtctaa
     5221 agaacagtcc gatcagtcgg aatgacccgc tgcgccgtgc caaacgttcc attgatcctg
     5281 cgccccagtt tccgtttgga cggcttgttt tctttacggg gctgcttgcg gttggaggaa
     5341 tgttcattta tcggttaagt gaaacgggcg ggcaaatgtc acaaactgcc tgcctcagtg
     5401 ccgcagccgt ttcattgctg tctgcccttg tcggcattat tcctgttgtc aaaacatggc
     5461 ggcgggagct tctttgggtt ctgctgggat tttttttatc cggcataatt cgtttattga
     5521 ttggcacact gggggtcgtt ataataatac tttttacaaa gatacaaagg atacaattta
     5581 tcgggtattt ggctttgttt tatacagcgt ttctggcggc cgatacatgg ctggccttat
     5641 ggattttgca gaattcgcgg atacaaaagc aggaaacggc ggtatatgga actatttggg
     5701 atattattgg caaatcttaa tccgcttaaa gaagtggtca gtaagccgat ttatgaattt
     5761 acggtcggcc gctggaccgt tgcatttacc aatcatatgt ttatgatagc cctggcctcc
     5821 gctgccctga tgatagggct accgctggcg gtccggcaaa agggtctggt gccgcggggc
     5881 tttaagaatg tcattgaaac gatctgtgtg ttcctgcggg aagacatggc acgtcccatg
     5941 ctcaaagagc ataccgaccg ctatatcagc gttctatgga cgatgttttt ctttattttg
     6001 acaatgaacc tgctgggact gacgccgctg gaccggtttg tctggctgtt taccaaaaaa
     6061 caaaactggg ggggagcggc aacggccaat atttatgtga ccggggcatt ggcaaccttt
     6121 gcctttctcc tgtttcatgc cgcaggcatc cggcagaagg gatttatcaa gtatctggca
     6181 accttgtcgc ctaaagtgcc gtggctgatg atgccgttta tgtttgtgat ggagctgctc
     6241 agttcgtttg tgcggctttt ttcgctggca attcggttgt ttgccaacat tctggccggc
     6301 catattctgc tggctgtgct gctgggtttt attataatgt tcaagaactt tatggtggct
     6361 tcggcctcta ttctggcaac caccctgatg agtctgctgg agctgtttgt agcattcctt
     6421 caggcctata tatttgtgtt tttaacgacg gtctttatta tgtttgctgt cagtgaagag
     6481 cattaaagcc ggcgttagaa tgtataaaaa gacaagaaaa accagttttt gtatgtggag
     6541 gtattaaccc atgttggatt tggcaacaat ggcaccgatg ctgctggccg aaattgttct
     6601 ggatgacaag gcattcggcc tgctgggcgg tcttttggga accggattga ttgtattggg
     6661 ggctgctcgg ggcatcgggc agctggccgg cagtgccgta gaggcaattg cacggcagcc
     6721 ggaagccggc aaccgaatct ttacgacgat gattattgca gcggcactga ttgaaggcgt
     6781 cacgctgttt gcgctgatta tctgtcttct ggcagtattg ggataatatc ttgcacaaag
     6841 ggaaccgtta tgcaggttcg cagaattgtt gcggttttgg tattgcttgt ctgcatctgc
     6901 ggacggctgg ctgcctctga aggcaccgcc gaaagcgaag ccgcccccag tgtgttcgaa
     6961 gggtatttcg gcgaatccat ctggacgctg gtctggtttg gcgtgctgct gggggtgctg
     7021 tggaaatttg cctggaagcc gcttctggcg gggctgacgg gccggcagga atatatcgaa
     7081 aggcaaatca gcgacgcaga aaaaacacgg gctgaagcca agaaagtgct ggaagaatac
     7141 ggcgccaaat tggccgatgc agaacggcag gggcgggata ttattgccgc ccgcatccgg
     7201 gatgcggaaa agcaggcgca ggaagtgcaa ttgaaaattc agcaggagat tgaacagcgc
     7261 aaagcccgcg ctgaggccga tttagaccgt gaacgcatcg aggccgaaga aaaactgtgg
     7321 gtgcaggccg gcgagattat ccggcggctg ggacaggatg tcttcggcaa agcccttgac
     7381 gatgaggata accagcggct gatacggcag gccattgacc ggctcaaaga agtcagccgc
     7441 cgctaagcga ggcgctctat ggcgcgaagc gcacgacata ttattcatga aatatacagc
     7501 gacgtgatgt tcgagctggc cgaagatgca ggcctgctcg aagaggtgat ggatgacctc
     7561 gaagcggtca gcaaggttct gcaggacgaa cccgattttt tgacgctgct gacgctggga
     7621 cagatcaagg aagacgaaaa ggccgctatg attcggcggg ttttcaaggg acgcatccat
     7681 gacctgacgc tcgatttttt gtgtgtgctg gcccggcgca accgcatggg ttttctgcac
     7741 ggcatcggcg accggtatca aacactgctc gatgagcgga aaaatattca ccgcatcgaa
     7801 gtaacgctgg ccaaggagcc gaccgaggag cagctcgaat cgctcaaacg ccagattcgc
     7861 gatgcgattc aggccgaaat caagctggct gttacggtcg atccggaaat tattggcggt
     7921 attattattc gcaagggcga cttggtaatc gataattcgg ttcgcacaat tctgaatcga
     7981 acggtcgacg ccattatgag ccgctccaag gcaaaaacca ctatcaaaag caataacgag
     8041 tcatccatat gaagtttgac atgaatgaaa tcagtacgct gattaaggaa gaagttcggc
     8101 gctaccgcac cgaagttgat gtggcacagg tcggttcggt gctggaagtg ggcgacggca
     8161 t
//
LOCUS       DAOTBO010000109         8160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7486_length_8160_cov_5.736212, whole genome shotgun sequence.
ACCESSION   DAOTBO010000109 DAOTBO010000000
VERSION     DAOTBO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_8160_cov_5.736212"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLE88_10765"
     CDS             complement(<1..258)
                     /locus_tag="PLE88_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM76988.1"
                     /translation="MTVETDAQPQRPLSVRQDIGIVIAEDDPGHYVLARNCLRSAGIT
                     NEILWFEDGQAVLDFFSKLDRKGGRYILLLDIRMPKVDGMKV"
     gene            complement(255..2000)
                     /locus_tag="PLE88_10770"
     CDS             complement(255..2000)
                     /locus_tag="PLE88_10770"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOM76989.1"
                     /translation="MTFRFLANNLAIMLAMGLFYGLLIRNRPRQLIQWQILCGFFFGL
                     AAIVTMTNSFVLRPGAIFDCRSVILSVTGMFAGPVPALIAAVLAALYRIWQGGVGAAT
                     GVSVIFSTTAVGLVFYYLRRKYAYLNKLLWLYVFGILVHIVMLLLMLTFPRSIAIEVY
                     KTITLPVLTLYPIGTILLSKIFIDQENRLNAEEQLRAANRQLKEGEAKLKNLLHLFES
                     SLNEIYIFEADTLHFIQANAGARRNIGYTLAELQQLTPLDLKPELTAQTFAEITEPLR
                     CGLKQIVVFETNHQRKDGSTYPVEVHLQMSVLDGRQVFVAFILDITERKKAERQQKRL
                     MRALEAKNRELQDIVYAASHDLKTPLINIAGFANLLSQYCARLRELGQSADPAVRSLF
                     ETDIPEALHFITQGAQKMKSLIDGLLQVARLGTYTPQLSRLDMNNLISSIITTAAFRI
                     QQTGAKVTADNLPPCVGDAGQINRVFTNLLDNALKYADPARPPRIHISGRHHDNQCVY
                     CVEDNGIGICPEHQDKVFELFHRLNPKGIIEGEGLGLTIVRRILNMHNGKVWVESVPG
                     QGSKFFVALPDKEDV"
     gene            complement(2223..3401)
                     /locus_tag="PLE88_10775"
     CDS             complement(2223..3401)
                     /locus_tag="PLE88_10775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOM76990.1"
                     /translation="MLPLRTSVIPKRTPYANYLLIAVNTVVFLLSTYLALDPRTGKHL
                     MALRPWAGPFMLHPEHPYIWQFVTYAFLHGSVMHIVGNMFFLYIFGNNVNDKLGNIGY
                     LCFYLASAVFSGIGHSLLSYNSVLGASGAVAAVTGAYLVLLPNSLITVVYWFFFIGTV
                     EIRALYFIALKLIFWDNILAADPRQGIAYSAHLAGYAFGIAAVLGLLSLGLIETNYND
                     LWSMLRQWNRRRQFRDTSGDSSFDKPVRTSVFSRTPIDGEQNSQIRERILELRSRISD
                     AMNSRLAPEAAALYLELLKIDDAQILPRQLQLDVANQLMSEGQWQLSADAYQKYLSQY
                     SHFGYAEQVHLMLGLLYGRYLNRFPEALKHLNMAKDHLTDPGQKNLCLQEIERLNHLV
                     "
     gene            3968..5068
                     /locus_tag="PLE88_10780"
     CDS             3968..5068
                     /locus_tag="PLE88_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOM76991.1"
                     /translation="MKSVPVEMGGKFVVGSANQEKTPQGNAEERYERAAKPAGSAGVR
                     SVHRQCGTNVAMIGCSDAFTSLLDTLESIAGRECCVMITGETGTGKEMAARKIHLLSR
                     RADKPFVPVDCTTLTGQLFESQLFGHVKGAFTGAIHDTLGFFRAADGGTIFLDEISEI
                     PLELQAKLLRVLQEGTVTPVGSTRSYPIDVRVLCATNRQLRTMVEKNEFRADLYYRIN
                     VINLEIPPLRQRPEDILPLADYFLQNLADFYAEPPKKLSSHAQHLLLNYSWPGNVRQL
                     ANAMERAYVLSRSQIIEPSALPSEILMSAYTAGPGRVLPTLDAAQEQLITEALRITNG
                     RKVAAARMLGIDRRRLNRIIEKLHIQVPKSND"
     gene            6104..6508
                     /locus_tag="PLE88_10785"
     CDS             6104..6508
                     /locus_tag="PLE88_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76992.1"
                     /translation="MLIFAKMNVLAVGIEQQAEALQGLPIRLLNLTHGSEAIHSFRND
                     PIDSVISHWNLVDMPDGQFLRSLRRIRPDMPTIAVIQPNNPQQEIEARMLGVSAVVCE
                     DSDSGYFRTVLTNVLGLQSAEAIETLYAVREA"
     gene            6882..7328
                     /locus_tag="PLE88_10790"
     CDS             6882..7328
                     /locus_tag="PLE88_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOM76993.1"
                     /translation="MGKIKHFSMLIWLLGGILMTAGCVERRLTIITEPQEAVVWLNDE
                     EIGTTPVTVGFNWYGDYNIRIEKPGYEILNTHRLLERPAHDKFPLDFFTEVLWPGRIK
                     DAYTWTFALQPYQQVSSEDLIQAAGRMKQKTDEELGKIAIEILSEK"
     gene            7359..>8160
                     /gene="mtnA"
                     /locus_tag="PLE88_10795"
     CDS             7359..>8160
                     /gene="mtnA"
                     /locus_tag="PLE88_10795"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOM76994.1"
                     /translation="MIETLKWIGGPDGRLELIDQRLLPGRYEKLCCRTPSQVYEAICT
                     LAVRGAPAIGIAAAYGVCLAVGAVKDTASAHQAVEAVEKACDHLAASRPTAVNLFWAL
                     QRMRSKASQVLAAAPSASARIIKDAMLEEANAICMQDRRACEAIGQNGLFIIPEGGAV
                     LTHCNAGALATAGIGTALAPMYLAHRLGRRFCVYVDETRPLLQGARLTAWELMQAGIE
                     AVLICDNMAATLMQQAKIHAVITGADRIAANGDTANKVGTLGLSIIARH"
BASE COUNT         2183 a         1985 c         2069 g         1923 t
ORIGIN      
        1 caccttcatc ccatcgactt tgggcatccg gatatccagc agcaggatat atcggcctcc
       61 tttgcgatct agtttcgaaa agaaatccag caccgcctgc ccgtcctcaa accagaggat
      121 ttcattggta atcccggcgc tgcggaggca gttccgggcc aaaacataat ggccgggatc
      181 atcctcggca atcacaatgc cgatgtcctg ccgaaccgac agcggccgct gcggttgggc
      241 gtctgtttca actgtcatac atcctcctta tccggcagag ccacgaagaa tttgctgcct
      301 tggccgggaa ccgattcgac ccagaccttc ccattatgca tattcagaat ccgacgcaca
      361 attgtcagcc ccagtccttc cccttcgata atccccttgg gattgaggcg gtggaacagc
      421 tcaaaaacct tgtcctgatg ctccggacag attccgatgc cgttgtcctc gacacagtaa
      481 acgcactggt tatcatgatg ccggcccgag atatggatgc gcggcgggcg ggcgggatcg
      541 gcgtatttga gggcattgtc cagcagattg gtaaagaccc ggttaatctg cccggcatcg
      601 ccgacgcagg gaggcaaatt gtcggcagtg accttggcgc cggtctgctg gatgcggaag
      661 gcagcggtgg tgataatgct gctgatcaga ttgttcatat cgaggcggct taattgaggg
      721 gtataagttc ccaagcgggc gacttgcaga agcccgtcga taagcgattt cattttttgg
      781 gcgccttggg tgataaaatg aagtgcttcg ggaatatcag tttcaaacaa ggatcgaacg
      841 gcggggtcag cagattggcc taattcccgc agacgggcgc agtattggct gaggaggttg
      901 gcaaatccag cgatattgat cagcggagtc ttgaggtcat gagaggcggc atagacaatg
      961 tcctgcagct ccctgttctt ggcctccaga gcccgcatca gccgcttctg ctgacgctcg
     1021 gctttcttcc gctcggtaat atcgagtatg aatgccacaa agacctgccg cccatccagc
     1081 acagacatct gcagatgcac ttcaaccgga taggttgaac catctttgcg ctggtgattt
     1141 gtttcgaaaa cgacaatctg cttgagaccg catcgcagcg gttctgttat ttcagcaaag
     1201 gtttgagccg tcagctctgg tttcaaatcc agcggcgtca gctgctgcag ttcagcaaga
     1261 gtatacccga tgtttctgcg ggcgccggca ttggcttgaa taaaatgcag cgtatcagcc
     1321 tcaaagatat agatttcatt cagggatgat tcgaagagat gaagcagatt tttaagtttt
     1381 gcctcccctt cttttaactg ccgattagcc gcccgcagct gctcttcagc gttcagacgg
     1441 ttttcctgat cgataaaaat tttgctcagc agaatcgttc cgatgggata caaagtcagt
     1501 accggcaggg tgatggtttt gtaaacttct atagctatgg atctgggaaa tgtcagcatc
     1561 agcagaagca tgacgatgtg aaccagtatt ccgaacacat agagccacaa gagcttgttc
     1621 aaataagcat actttctccg caggtagtaa aagaccagtc ccaccgctgt agtcgaaaaa
     1681 atgaccgaca cccctgttgc tgctccaacg cctccctgcc agatgcgata cagcgccgcc
     1741 aaaacagccg caatcagcgc cggcaccggc ccggcaaaca ttccagtaac gctgagaatg
     1801 accgaacggc agtcaaaaat tgctcccggc cgaagcacaa agctgtttgt cattgttaca
     1861 atagctgcaa gtccgaagaa aaagccgcac agaatctgcc attgaatgag ctgccggggt
     1921 ctgttgcgaa tcagcaggcc gtaaaataaa cccattgcca gcattatcgc aaggttattt
     1981 gccaaaaacc tgaaggtcat aattctgtct tccttctagc caaaagatgc ggtagattat
     2041 aagtattctg cttttcaaaa acaagaggat tttgaaagtt ggcaaatgtt ctattgtgga
     2101 aatgcaggtc tgaaataggc aattaaatac tgcttcggaa cagccggcca gaaacacgta
     2161 aacggctgtt tccttttgat aaaaatattt caatcagctt gtgtgaaaag acattgcgcg
     2221 cattaaacaa gatgattgag ccgttcaatt tcctgcaaac agagattttt ctgccccgga
     2281 tcggtcaagt ggtctttggc catattcaga tgttttaggg cctctggaaa gcggtttaaa
     2341 tagcgtccgt aaagaagccc cagcatcaga tgaacctgtt cagcgtagcc gaaatggctg
     2401 tactggctta aatatttttg ataggcatca gcggaaagct gccactgtcc ttcggacata
     2461 agctgattgg ccacatccag ttgaagctga cggggcagga tttgcgcatc atcaatcttg
     2521 agcaattcga ggtagagagc cgctgcttca ggtgctaaac ggctgttcat cgcatcactg
     2581 attcggctgc gcaattcaag gatgcgttcc cgaatctgac tgttttgctc gccgtcaatg
     2641 ggagtacggc tgaaaacact tgtccgaacg ggcttatcaa aagaactgtc gccggaagtg
     2701 tcgcggaatt gccggcggcg attccattgc cgcagcatag accacaggtc attgtaattg
     2761 gtttcaatca gtcccagcga cagcagaccc agtaccgctg caattccaaa cgcatagccg
     2821 gccaaatgag cgctgtaggc gattccctga cgcgggtcgg cggcaaggat gttatcccag
     2881 aaaatcaatt taagagcaat aaaatacaga gcacgaatct cgacagtgcc gataaagaaa
     2941 aaccagtaaa ccactgtaat taaactgttg ggcagcagaa ccaaatatgc tcctgtaacg
     3001 gccgctacag ctccggaagc ccccaaaacc gaattataac tcagcaggga atggccgatg
     3061 ccggaaaaga ccgcgctggc aagatagaaa cacaagtagc caatattgcc cagcttgtcg
     3121 ttgacattgt tgccgaagat atataaaaaa aacatattgc cgacaatatg cataacgctg
     3181 ccgtgcaaaa aagcatatgt aacaaactgc cagatgtagg gatgttccgg atggagcata
     3241 aacggtccag cccacgggcg aagcgccatt agatgtttgc cggttctggg atccagcgca
     3301 aggtaagtgc tcagcagaaa caccaccgtg ttgactgcaa tcagcagata attagcatac
     3361 ggagttcgtt ttggtattac acttgtacgc agcggcagca tatgcgttat acaggattaa
     3421 ggtttcgatt atttctattc attcagctgc cgcattgtac agggtttgag caggcaggac
     3481 aagaaaataa aaaagaacgg gacagatacc cgcctgcagc aggcagctgc cccgtcgagc
     3541 gtccgtgcaa ggaaaagaca ggagaggaga ttgtcctgaa taaggtctaa tcgtataaat
     3601 tgttctctcc atccaaaaca taaaaaataa aacggatatc tgcaaacacc gaaaaatttt
     3661 ttttgcctat tcaaaaaagg aaagaaaacc aacggaaaga gaattgcagg cacaataaca
     3721 gtaataccag acatttaatg ttgtaatatg gctgtctaag gagtccaatt caataaaaaa
     3781 tgatgagctg cgcgttacaa tagaagtgaa caagcgacaa agtcaatctt gtatttcaga
     3841 aaaatgttcg cacactcttg caattggata tgtaaaagac tataatgctg ttttggaggc
     3901 atgaggaaaa gacaagggca cagacttttc tcattgcacc cgtcacccgc atcgtttcaa
     3961 aaagacgatg aaatctgtgc ctgtggaaat ggggggaaag tttgtagtgg gaagcgccaa
     4021 tcaggaaaag acgccgcaag ggaatgcgga ggaaagatat gaacgcgcag ccaagccggc
     4081 cggcagcgcc ggagtgcgca gtgtgcatag acagtgcggc accaatgttg caatgattgg
     4141 ctgttcagat gcttttacgt ccctgttgga taccttggag tcgatcgcgg ggcgagagtg
     4201 ctgcgtgatg attacaggag agaccggcac gggcaaggaa atggcggccc gcaaaattca
     4261 cctgctcagc cgccgtgctg acaagccctt tgttccagtc gattgtacaa ccctgacagg
     4321 acagcttttt gaaagccagc ttttcggcca tgtcaaggga gcatttacgg gggctattca
     4381 cgatacgctg ggttttttcc gggctgccga tggggggact atttttctgg atgaaatcag
     4441 tgaaatccct ctcgaacttc aggccaaact ccttcgggtt ctgcaggaag gcacagttac
     4501 ccctgtcggt tctacccgca gttatccgat tgatgtgcgg gtgctttgtg caaccaaccg
     4561 ccagctgcgg acgatggtgg agaaaaacga atttcgggcg gatttgtatt accgcatcaa
     4621 tgtgattaac ttggaaatac cccctctccg gcagcggccg gaagatattt tgccgttggc
     4681 cgattatttt cttcaaaatt tggctgattt ttacgccgag ccgcccaaaa aattgtcatc
     4741 ccacgcccag catctgctgc tcaattattc atggccgggc aatgtccgcc agcttgccaa
     4801 cgcaatggaa cgggcttatg tgctcagccg cagccagatc atagaaccct cagcgcttcc
     4861 ttctgagatt ctgatgtcgg cctacacagc cggacccgga cgagtcctgc ccacgctgga
     4921 tgcggcgcaa gagcagctta ttaccgaagc ccttcgtata acaaacggca gaaaagtcgc
     4981 agccgcgcga atgcttggca ttgaccggcg ccgcctcaac cgcatcatag aaaagctcca
     5041 tattcaggtt cccaagtcaa acgattgaca actcttcttt tttgctgctt ttgtgtcatt
     5101 gacatagagc agcccaatga tggatttttt ctcgagcccc cgcaggagga gttttttctt
     5161 ctgcctaaat gtcctataat cccaatcccc aacagccttg ctccatttta gcctattagt
     5221 ccattctggt cggatatgtt ctatcatttt gtaatataag tacttacaga aattacaatc
     5281 agtcagaaag ggctaaagca gtacaaaata gaaaaaattg gtccggtgta tttgtgtccg
     5341 ataaaaaatt ttaagttaat gcagcaaaag agcttaagaa cagaatagaa aaaatttatc
     5401 cggctggcac gtaagttgaa taaatagtcc ttatgagttg gacataaaaa gccgaaatca
     5461 ggcatgaaga aagctcttta acaaatgaaa agatgacagt gctgtgatga gcaatttgtg
     5521 caacggctct cagcggtgct gatgatgctt cttgcgacga aggaaggtga agagaacctg
     5581 gctgggcggg ggaataaccc atgatgagat gaaagctcgt tttaacgagt gaacgatatc
     5641 tttcctcctc ctccaaaggt cgggcagtta gccaatctcc ggttgaatgc ccgacatgca
     5701 aattatcccg ccggcaaagg cgggttgacg atatctttcc tcctcctcca aaggtcgggc
     5761 agttagccaa tctccggttg agtgcccgac atacaaataa tcccgccgat agaggcgggt
     5821 tgacgatatc tttcctcctc ctccaaaagt cgggcagttg gccaatctcc ggttgagtgc
     5881 ccgacataca aataatcccg ccgatagagg cgggttgacg atatctttcc tcctcctcca
     5941 aaagtcgggc agttggccaa tctccggttg agtgcccgac gtgcagataa ccccgccggc
     6001 aaaggcgggc ttcacatatt ttttcaaaac ggacttgccg cccgtgttcc gggcagcagg
     6061 cccgttcttt acaaacacaa caaagcggta tgggaataac cggatgctta tttttgcaaa
     6121 aatgaatgtt ttagcggtag gaatcgaaca gcaggccgag gccctgcaag ggcttcctat
     6181 ccgcctgctg aacctaacgc acggcagcga ggcgattcat tcgttccgaa acgatcctat
     6241 cgacagcgta atcagccatt ggaatttggt tgatatgccc gatgggcaat ttcttcgcag
     6301 tcttcgaaga atcagacccg atatgccgac tattgcagtc attcagccca ataatcccca
     6361 gcaggaaata gaagcccgca tgctgggtgt cagtgcggtt gtttgcgaag acagcgacag
     6421 cggctatttt cgcactgttc tgacgaatgt cttgggcttg cagagtgcag aggcgataga
     6481 aacactttat gcggtaagag aggcctgact ggccgcataa tatattcaag aaaaaatgtc
     6541 ttgccgatgg atgccgcata aacggcattt ttttgcttcc agttaaaaat gtctgttcca
     6601 atcctttttt gcatattatt gattggctgt atttgaagat ataatttacc catatatcaa
     6661 ttccttctaa tccgtccaaa gtacttctgc agatagaggc agcctctgga aataacccta
     6721 aaaagagcct tcagttattt tagctgctga acgggccggt aaaataatgc cctcaatgcg
     6781 gattgaatcg ctcgaagaga aacggaaaat caatttgctt ttttatccgt tggccggtac
     6841 tttttaatgg attgagatga caacaagcac gggagattaa aatgggtaaa ataaagcatt
     6901 tttcgatgct gatatggctt cttggcggga ttcttatgac agccggctgt gtggagcggc
     6961 gactgacgat tatcacagaa ccacaggagg ccgtggtttg gctcaatgat gaggaaatag
     7021 gtacaactcc ggttacagtg ggattcaact ggtacggtga ttacaacatc cgcattgaga
     7081 agccgggcta cgaaatcctc aatacccatc gcctcttgga acggcctgcc catgataaat
     7141 tcccgctgga tttttttacc gaagtactct ggccggggcg aattaaagat gcctatactt
     7201 ggacatttgc cctccagccc tatcagcagg tttcttctga agatttgata caagccgcag
     7261 gccgcatgaa gcagaagacc gacgaggaac tgggtaaaat tgccattgaa attttgagcg
     7321 aaaaataacc gccgaaaaat accacagacg tgcagggcgt gatagaaaca ctcaaatgga
     7381 tcgggggtcc ggacgggcgg ctggagctta tcgaccagcg gcttctgccg ggccgatacg
     7441 aaaaactctg ctgccgaaca ccctcccaag tctatgaagc catctgtaca ttggctgtgc
     7501 gcggagctcc ggcaattggg attgccgcgg cctatggggt ctgtcttgcc gtcggggcag
     7561 taaaggacac ggcctctgca catcaggccg tagaagcagt ggagaaggcc tgtgatcatc
     7621 tggccgcaag ccgaccgaca gcagtcaacc tgttttgggc attgcagcgc atgcgcagca
     7681 aggcgagtca ggttttggca gctgctcctt ctgcttcagc ccgaatcata aaagatgcaa
     7741 tgcttgaaga agcgaatgca atttgcatgc aggatcggcg tgcctgcgag gccatcgggc
     7801 aaaacggcct gttcatcata ccggaaggag gggcagtttt aacccactgc aacgccggcg
     7861 cgctggccac ggcaggcatc ggcaccgcat tggctccgat gtatcttgcc catcggctgg
     7921 gcaggcgatt ctgcgtctat gtggacgaaa cacgcccgct gcttcagggg gcacgcctga
     7981 cagcatggga actgatgcag gccggcattg aggcagtctt gatttgcgat aatatggccg
     8041 caactctgat gcagcaggcc aaaatccatg ctgttataac cggtgctgac cgtatcgcag
     8101 ccaatggcga caccgccaat aaggttggca cgctgggcct aagcattata gcaagacatt
//
LOCUS       DAOTBO010000110         8130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7523_length_8130_cov_5.394427, whole genome shotgun sequence.
ACCESSION   DAOTBO010000110 DAOTBO010000000
VERSION     DAOTBO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_8130_cov_5.394427"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..475)
                     /locus_tag="PLE88_10800"
     CDS             complement(11..475)
                     /locus_tag="PLE88_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HOM76995.1"
                     /translation="MGPELEKAINELSLRMRMLRAIQEDAAPADALTEREWLILQLLA
                     EQGQMTVTQIAAAWPNVSESTISMTITKLWRKRGLVSKTIDPQNQRVTQVALTDKGKK
                     ELETFLQQRSQRFKALFESLQMSPQEREVLIRICQRGVKVLDQYLQMTSDRK"
     gene            complement(728..949)
                     /locus_tag="PLE88_10805"
     CDS             complement(728..949)
                     /locus_tag="PLE88_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOM76996.1"
                     /translation="MPIFEYKCPQCGFINEVLVKSSDTLAPACPQCGHKKTEKQFSSF
                     TPVVKQTPSSRPAKCHSCPSAGSCPSFEK"
     gene            complement(1067..1732)
                     /locus_tag="PLE88_10810"
     CDS             complement(1067..1732)
                     /locus_tag="PLE88_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76997.1"
                     /translation="MLLVPVLGAVFFSVGRPSDTFFDITLSDWSDFYGELLGFSQPEV
                     WIIWLIIVVLLIVQASLLIIPVRVTHGRPKPQRGIWWTAAMAALLYTMLLFFAALSIM
                     AAVWGDDMPETLFWIIVAIVPVNWIVWLAVFGLFSKSLDPQSFIRRLMQRLIAVPSHI
                     IVRHRDVCCAHGLTAAGITAGLAVLFFAFGPGLYFLYADRIRSRRPNLSPSPPQPESQ
                     PQG"
     gene            complement(1677..2759)
                     /locus_tag="PLE88_10815"
     CDS             complement(1677..2759)
                     /locus_tag="PLE88_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM76998.1"
                     /translation="MKRTNRLVVISFALLAGFSFLFASGQGTKGVLLLPEGISVELYS
                     SQNGTLMLSAKDGRAELPQGPYRLESWTLEKADPQGRVWKLSGDLTTAKMIDIGPEPV
                     QLDIQAEPIACKLNVVMTSQTVNFELSLNGPNGERLRVDGPGVKRDNLPQPAFVLYNS
                     DRSFLQTYDFQSQCCGNYRLNWTPPQGVQGPFWIDVKVTGPFTLDFQPKTLDELTEET
                     IAKMQAGQSGSSPDSPLRGGWDSAGYGDSGFCGGEVLQTCLAGTGQIQQDNPTADCCA
                     GGCCSLSFAGYAAAGGHWPIQCSDSCPSNSFHFSRYSDWRTAGQACSQGTGQMAVCGD
                     STVWIFIRRAVGACSGGGIFFGRPSV"
     gene            3035..3781
                     /locus_tag="PLE88_10820"
     CDS             3035..3781
                     /locus_tag="PLE88_10820"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOM76999.1"
                     /translation="MKKHVWQTGILMFALAAYAAQQATQWEVHDRRRPNPPVIAPGAE
                     NHLPPSDAVVLFDGKDLSAWKSAKDGSPAKWKVENGYMEVTKTGDIQTREAFGSCQLH
                     IEWACPEVVSGSDQGRGNSGIFLMDKYEVQVLDCFDNVTYADGQTAAIYGQKPPLVNA
                     CRKPGQWQTYDIIFHAPKFDGQAVVRPATITVLHNGVLVQDHWEIKGSTFHKMPAAYE
                     PHPDKMPLRLQDHGNPVRFRNIWIRPLADE"
     gene            3812..5038
                     /locus_tag="PLE88_10825"
     CDS             3812..5038
                     /locus_tag="PLE88_10825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOM77000.1"
                     /translation="MKFTELNLKPEILKALDKKGYTELTPIQEQTFPHVLAGKDLIAL
                     AQTGSGKTAACAIPVVQSIDPSLNEVQALILVPTRELALQYVDEISWIAAETGVSAFA
                     VYGGFSMEIQKSKLAHGVQALVATPGRLIDLLYNSPLRLANVRYFVLDEADEMLNQGF
                     LSDIEFIFSCMVHDHHTMLFSATMPPEIKQLAKRYLKDPVLIELNLDQKAPESLSHYF
                     TLVQPHQRFEKLLELIQHEKPAQAIMFCSSRTSCQKLYDQLKKKVKSVELIHGGLDQN
                     KRTSLFRRFKKLDIQYLAASDIAARGLDFSHTTHIFNYDLPHNPEDYTHRTGRTARMG
                     RQGKAITLVTAKDLRKLAQILRANRIEPLWIGPAPDLTAVSRKKKQAGSGRSRCRQRP
                     AGHFRRRSDSKKKKSE"
     gene            5043..5615
                     /locus_tag="PLE88_10830"
     CDS             5043..5615
                     /locus_tag="PLE88_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOM77001.1"
                     /translation="MESVKHYIQQMLTLHKTLLTAFEADCLGAVESAAGLLTDCLRGG
                     GCIYLCGNGGSAADAQHAAAELVGRFAQQRKGLPAVALTTDTSILTSVGNDYGFEQIF
                     SRQVEALVHPGDVLWAFSTSGSSPNILAAAALARQKGARVLAFTGRKHSPLEALADVC
                     ICVEGPTAAVQEIHQTAYHILCGLAEKALA"
     gene            complement(5639..6772)
                     /locus_tag="PLE88_10835"
     CDS             complement(5639..6772)
                     /locus_tag="PLE88_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOM77002.1"
                     /translation="MKADNTHSNILTRAILSVLMLSGGLALADDLYLNGGIFAEIDST
                     INGSVFIDDATVHMLDNAHIMGFVVTSSGAVLGIYGGQIDYMLLISTADPSLPEGLVT
                     IYGTDFAVDGVPVDPATTELFLQGQVLSGVYASGTPFAIVVDCAISGNANNIYYQTIK
                     LGWLASQPDIALSQTACDFGSVEIGTAAACTATISNVGNAALTILSLALTQDSLMQFG
                     ITPWQVLPVTLAPNESIDIEFLFGPVAEGPAQAVVHIASSDPDNPVVDIVLTGQGIPA
                     QLTPQQQIAAIADFYALAIDDGTLYGVGKGKSAANKAETFEKMIGLAADLIGAGQTAE
                     ALDALCMIAAKCDGLKSPADFVEGTAAAALHAMITDLIDALRR"
     gene            7030..7533
                     /locus_tag="PLE88_10840"
     CDS             7030..7533
                     /locus_tag="PLE88_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM77003.1"
                     /translation="MTKNGFTLIELMIVVAILGILAAIVLPQVEGHTVQAKEAAVKDT
                     LYTVRTQIQLYKLQHNGLAPGYINTTQATPTILGYQLTGTSAMNGLAVSSKVPSTTYP
                     FGPYLSSLPVNPYNNLSTIKMVSAETTDFSTAADNTTGWLYQKETATFKINKTGTDSQ
                     GVPYVNY"
     gene            complement(7585..7911)
                     /locus_tag="PLE88_10845"
     CDS             complement(7585..7911)
                     /locus_tag="PLE88_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOM77004.1"
                     /translation="MSEKKAFVVELNSQKGYQRLIPGPPATCGMKVGRVYLEPGADCG
                     LHSTEDKEETLVFLAGSGTAVIAGQKLEVGQGRVCYIPPQTQHNICNTGSEPLIYIFC
                     VSPAAR"
     gene            complement(7932..>8130)
                     /locus_tag="PLE88_10850"
     CDS             complement(7932..>8130)
                     /locus_tag="PLE88_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77005.1"
                     /translation="GELAQGAAAVRQMGAAVEQLADYCLQIPQEPPAELVLVVCRKMH
                     STPAKLPRAFGQIKKRPLTDN"
BASE COUNT         1911 a         2201 c         2218 g         1800 t
ORIGIN      
        1 tatgaacttt ttattttctg tccgaggtca tctgcaaata ctggtctaag accttgacgc
       61 ctcgctggca gatgcgaatc aatacctcgc gttcctgcgg actcatctgg agcgattcaa
      121 acagcgcctt gaatcgctga cttcgctgct gcaggaaggt ttccaattcc tttttgccct
      181 tgtcggtcag tgccacctgc gtaacacgct ggttttgagg atcgatggtt ttggaaacca
      241 gcccgcgctt tcgccagagc tttgtaattg tcatagaaat cgtgctttca ctgacattcg
      301 gccaagcggc ggcaatctgg gtgacggtca tctgaccctg ttcagccaga agctgcagaa
      361 tcagccattc ccgttccgtc agcgcatcag ccggtgcagc gtcttcctga atcgcacgaa
      421 gcattcgcat ccgcaaactc agctcgttta ttgccttttc aagttccggg cccattgtgt
      481 tttctccaag ctcggccgaa cgtccctgtt ctacaatatt atacgctctg tttttaattt
      541 ttatttaaat attttaacat gtcaacagtt taatttttga actgttctca tataaaacat
      601 ttatatcgtc ataaatattt atatattaag attttatatt aatgtgacca gcgctgccaa
      661 gtgagaaaaa aaataaaaaa tttttccaga caggcaattt tttttgttat ttcatgcaga
      721 agaaagatta cttttcaaaa ctcgggcatg agccggcact ggggcagcta tggcattttg
      781 ccggcctgct ggaaggggtt tgtttgacaa cgggggtaaa actggagaac tgcttttcgg
      841 tttttttgtg tccgcattgg ggacaggccg gagccagagt gtcagacgac ttgaccagca
      901 cttcattgat aaacccgcat tggggacatt tgtactcgaa tatcggcata gttttatcct
      961 aaataatatt gcatcaacag ttttttcagc ccgatatcat aaccgaagca tctgatttgt
     1021 caaaggttct cggcggttct ttgccggctg atctatcggg gcaggattat ccctgcggtt
     1081 gggattcggg ctggggcggt gatggagaca ggtttggccg gcggctgcgg atgcggtcgg
     1141 catagagaaa atacaagccc gggccgaacg caaaaaacag taccgccagt cctgccgtaa
     1201 tgccggcagc cgtcaggccg tgtgcacagc agacatcccg gtgccgcaca atgatatgac
     1261 ttggcacggc aatcagccgc tgcatcagcc ggcggatgaa actttgcggg tccagcgact
     1321 tgctgaacag cccgaacacc gccagccata caatccagtt gaccggcaca atggccacaa
     1381 ttatccagaa cagcgtctcg ggcatatcat cgccccatac ggcggccatg attgacagcg
     1441 ccgcaaaaaa cagcagcatc gtatacagca gtgcagccat cgccgccgtc caccagatgc
     1501 cccgctgcgg cttgggacgg ccgtgcgtca cccgcacggg gataatcagc agcgacgcct
     1561 gaacaatcag cagcaccacg ataatcagcc agataatcca tacctccggc tgcgaaaagc
     1621 ccaacagttc cccgtaaaaa tcgctccaat cggataaagt gatatcgaaa aaagtatcag
     1681 acggacggcc gaccgaaaaa aataccgccc ccagaacagg caccaacagc acgacgaata
     1741 aaaatccata cagtagaatc gccgcaaacg gccatttgac cggtgccttg cgaacaagcc
     1801 tgaccagcag tccgccaatc agaataacgg ctaaaatgga aagaatttga agggcagctg
     1861 tctgaacact ggattggcca atggccgcct gcagcagcat atccagcaaa agacagactg
     1921 cagcagccgc cagcgcaaca gtcagccgtt ggattgtctt gctgtatttg gcctgttccg
     1981 gccaaacagg tttgtaaaac ttcgccacca caaaaaccgc tatcgccata accagcagaa
     2041 tcccagccgc cgcgcaaagg actgtcagga gaactgcccg attggcctgc ctgcatcttg
     2101 gctatggttt cctcggttaa ttcgtctaag gttttgggct gaaagtccag cgtaaacggg
     2161 ccggttacct tgacgtcaat ccagaacggt ccctgcacgc cctgcggcgg cgtccaattc
     2221 aatcggtaat tgccgcagca ctgggattga aaatcatagg tctgcaagaa gctgcggtcg
     2281 ctattgtaca gcacaaaagc cggctgcgga agattatcgc gcttgacgcc ggggccatcc
     2341 acccgcagac gctcgccgtt aggaccgttc agcgacagct caaaattgac ggtttggctt
     2401 gtcatgacga cattcagttt gcaggcgatc ggctcggctt gtatatccag ctgcaccggc
     2461 tcggggccga tatctatcat cttggctgtc gtcaggtctc cggataattt ccaaacgcgc
     2521 ccctgcggat ctgccttttc cagagtccag ctttccagac ggtagggtcc ctgcggcagc
     2581 tctgccctgc cgtctttggc gctgagcatc agtgttccgt tctgggatga gtaaagctcc
     2641 acgctgatgc cttccggcag cagcagaacg cccttggttc cttggccgga tgcaaaaaga
     2701 aaactgaatc ctgcaagcaa tgcaaaagag atgacgacca gcctgttggt ccttttcata
     2761 agcggtctcc ttttttaagc ggcaaaatgc accctgtgga taatcagaag tgtatcgact
     2821 tttctgccga aaacaaccgt aaaaattttc gttttgccgc agcgatcgcc ggctgatggg
     2881 actgccggcg gctgggaatc agctctaaac ccttgaccag cagaggatgc ggccggtttt
     2941 ttttatagga aatcggcggc cggatcggta taatttcctt ctgtaaaatg gacttgcgac
     3001 aaagagaatc tattacaagc agcggaggtt tattatgaaa aagcacgttt ggcaaaccgg
     3061 tattctgatg ttcgctctgg cggcctatgc agcgcagcag gcaacgcagt gggaagtaca
     3121 cgaccggagg cgtccgaatc cgccggtgat tgcgccgggg gctgaaaacc atctgccgcc
     3181 gtcggatgcc gtggttttgt ttgacggcaa agacctgtcg gcttggaaga gcgccaaaga
     3241 cggcagtcca gccaaatgga aggtggaaaa cgggtatatg gaagtaacca agaccggcga
     3301 catccagacg cgggaggctt tcgggtcgtg ccagcttcat atcgaatggg cctgcccgga
     3361 ggttgtctcc ggcagcgatc aggggcgggg caacagcggg atctttctga tggacaaata
     3421 cgaagtgcag gtgctggact gcttcgacaa tgtaacctat gccgacggcc agacggccgc
     3481 catttacggg cagaagcccc cgctggtcaa tgcctgccgc aaaccgggac agtggcagac
     3541 gtacgacatt atctttcatg ccccgaaatt tgacggtcag gccgtcgtgc ggccggccac
     3601 catcacggtt ctgcacaacg gcgtgctggt gcaggaccac tgggaaatca agggcagcac
     3661 ttttcataag atgccggctg cctacgagcc gcatccggac aagatgccgc tgcggctgca
     3721 ggaccacggc aatcccgtgc ggttccgcaa tatctggatt cgcccgctgg cggatgaata
     3781 aacaacccgc agtttgacag gacggacacc tgtgaagttc acggaactga acctcaagcc
     3841 ggaaatcctc aaggcgctgg acaaaaaggg gtataccgag ctgacgccga ttcaggagca
     3901 gacattcccg catgtgctgg cgggcaaaga cctcattgcg ctggcgcaga cgggctcggg
     3961 caagacggcc gcctgtgcca ttccggtggt gcagtcgatt gatccgtcgc tcaacgaggt
     4021 gcaggcgctg attctggtgc caacgcggga gctggcgctg cagtatgtcg atgagatttc
     4081 gtggatagcg gccgagacgg gcgtcagcgc gtttgcggtt tacggcggct tttcgatgga
     4141 gattcagaag agcaagctgg cccacggggt tcaggcgctg gtggccacgc cggggcggct
     4201 gattgatttg ctgtacaatt cgcccctgcg gctggccaat gtgcggtatt tcgtgctcga
     4261 cgaggccgat gagatgctca atcagggatt tctgtcggat attgagttta tcttttcctg
     4321 catggtgcac gaccaccata cgatgctgtt cagtgcgacc atgccgccgg aaatcaagca
     4381 gctggccaaa cgctatctga aagatccggt tctgattgag ctgaaccttg accagaaagc
     4441 gccggaatcg ctgagtcact attttacgct tgtccagccg catcagcggt ttgagaagct
     4501 gctggaattg atccaacatg aaaagcccgc acaggccatt atgttctgct ccagccgcac
     4561 aagctgccag aagctctatg accagctgaa gaaaaaagtc aaatcggtag aactgattca
     4621 cggcgggctg gaccaaaaca agcgcaccag tctgttccgg cggttcaaga agctggatat
     4681 tcagtatctg gcggcgtcgg atatagcagc gcggggattg gacttttcgc atacgacgca
     4741 tattttcaac tatgaccttc cgcacaatcc ggaagattac acccaccgca ccggccggac
     4801 tgccagaatg ggccggcagg gcaaggccat tacgctggtc acggccaaag accttcgcaa
     4861 gctggcccaa atcctgcggg ccaaccgcat tgaaccgctc tggatcgggc cggcgccgga
     4921 tttaacagct gtcagccgaa agaaaaaaca agccggttca ggccgcagcc gttgccgcca
     4981 gcggccggca ggacactttc gaaggcggtc ggattcgaaa aaaaagaaaa gcgagtgagt
     5041 ttatggaatc tgtcaaacac tatatccaac agatgctcac cctgcacaaa acgctgctga
     5101 cagcgtttga ggccgattgt ctgggggcgg ttgagtcggc agccggtctg ctgacagact
     5161 gtcttcgggg cgggggctgc atttatctgt gcggcaacgg cggctctgct gccgatgccc
     5221 agcatgccgc tgccgaactg gtcgggcggt ttgcgcagca gcgcaaaggg ctgccggccg
     5281 tggcgctgac gaccgacaca tcgattctga cctctgtggg caatgattac ggctttgagc
     5341 agatattcag ccggcaggtt gaggcgctgg tccatccggg ggatgtgctg tgggcgtttt
     5401 caaccagcgg cagcagtcca aacattcttg ccgccgccgc actggcccgg cagaaagggg
     5461 cccgggtgct ggcgtttacc ggcagaaagc attcgccgct ggaggcgctg gcggatgtgt
     5521 gcatctgcgt ggaagggccg acagcggccg tgcaggaaat ccaccagacc gcataccata
     5581 ttctctgcgg tctggcggaa aaggctcttg cctaaaaagg cggaatgcag cgggcttttt
     5641 agcggcgcag tgcgtcgatc aaatcggtaa tcatcgcatg gagagctgcc gcggcggtgc
     5701 cttcgacgaa gtcagcaggc gatttgaggc cgtcgcattt ggctgcaatc atacacagcg
     5761 catcgagggc ttcggcggtc tgtccggcgc cgatcaaatc ggctgccagc ccaatcatct
     5821 tttcaaaggt ttctgccttg ttggcggctg atttgccttt gccgacaccg taaagggttc
     5881 cgtcgtctat cgccagagcg taaaagtccg cgatggcggc tatctgctgc tggggcgtca
     5941 attgcgcagg gattccctga ccggtcagca caatatcaac cacaggattg tcagggtcgc
     6001 tgctggcgat atgcacaacc gcctgtgcag gcccttcggc aacagggcca aacagaaact
     6061 cgatatcgat ggattcattg ggcgccagtg ttacaggcag aacctgccaa ggcgtaatgc
     6121 cgaactgcat caaactatcc tgtgtcagcg ctagcgacaa aatggtcagc gctgcattgc
     6181 ccacgttcga gatagtcgcc gtgcaggcag ccgctgtgcc gatttcaacc gagccgaaat
     6241 cgcaggctgt ctgcgacagg gcaatatcgg gctggctggc cagccagccg agcttgatgg
     6301 tctgataata aatgttattg gcattgcccg aaatcgcaca gtccaccacg attgcaaaag
     6361 gagttcccga ggcatatacg ccggaaagaa cctgcccctg caaaaacagc tctgtcgtcg
     6421 cggggtcaac aggcacgccg tccactgcaa aatccgttcc gtaaatggtg acaagcccct
     6481 cgggcagaga aggatcggcg gtggaaatca gcagcatata gtcgatttgt ccgccgtaaa
     6541 tacccagaac cgcacccgag ctggttacca caaaacccat aatatgggca ttgtccaaca
     6601 tatggactgt ggcatcatca ataaacaccg agccgttgat ggtgctgtcg atttcagcaa
     6661 aaatgccgcc gttgagatag aggtcatcgg ccagagcaag tccgccagac agcataagta
     6721 ccgataatat cgctctggtc aggatattcg aatgggtatt gtcagctttc ataaaatccc
     6781 ccgtttatta aaggttaaat cattttttta aagagactcg cattgctata taaaatataa
     6841 cctgaaaaaa ggcaagtgca aatattttct taatattttc ttaatgtttt tgatgggtta
     6901 tttttgtccg gcgggattta tttattatgt tttctatgtg gtcagcagaa gaaggacgtc
     6961 ggtgaatgca gttccgataa ctgtgtgtac cttgggcaag tacgtctatg ggccaaacag
     7021 gcggtctgaa tgacaaagaa tgggtttaca ttaatcgaat tgatgattgt ggttgccatc
     7081 ctcggcatcc ttgcggcgat tgttctgcct caggtggaag gccataccgt gcaggccaaa
     7141 gaggcggctg tcaaggacac tctttacacc gtccgcacgc agattcagct ctacaaactc
     7201 cagcataacg ggctggctcc cggttatatc aacaccactc aggcaacgcc tacgattctg
     7261 ggctatcagc tgacgggcac atcggcaatg aacggattgg cggtgtcgtc caaagtgccc
     7321 agcacaacct atcccttcgg gccttatctg tcgtcgctgc cggtcaatcc ctacaacaac
     7381 ttatcaacta ttaaaatggt ctccgcggag acaaccgatt tctcaaccgc cgccgacaac
     7441 accaccggct ggctgtatca gaaagaaacg gccactttta aaatcaacaa aaccggcacg
     7501 gattcgcaag gtgtgcctta tgtgaattac tgaccgatcg gtcagttcgt ccagccatcc
     7561 ccgcggccgg cttgccgggc tgcttcagcg ggcagccggc gagacgcaga agatgtaaat
     7621 cagcggctcg ctgccggtat tgcagatgtt gtgctgcgtc tgcgggggaa tatagcagac
     7681 cctgccttgg ccgacctcca gtttttggcc ggcgataact gctgtgccgc ttccagcaag
     7741 aaacaccagc gtttcttcct tgtcttcggt gctgtgcagg ccgcaatcgg cgccgggctc
     7801 cagatagacc cgcccgacct tcattccgca ggttgccggc gggccgggaa tgagacgctg
     7861 atagcccttt tggctgttga gttccacgac aaatgctttt ttttcgctca taatgccttt
     7921 ctaaaaatat gtcagttgtc tgtcagcggc cgcttcttta tctgaccgaa tgcacggggc
     7981 agcttggccg gcgtggaatg catcttgcgg catacgacca gcaccaattc cgccggcggc
     8041 tcctgcggaa tctgcaggca atagtctgcc agctgctcca cagcggctcc catctgccta
     8101 acagccgccg caccctgtgc cagctcgccg
//
LOCUS       DAOTBO010000111         8022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7664_length_8022_cov_4.607883, whole genome shotgun sequence.
ACCESSION   DAOTBO010000111 DAOTBO010000000
VERSION     DAOTBO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_8022_cov_4.607883"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLE88_10855"
     CDS             <1..451
                     /locus_tag="PLE88_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77006.1"
                     /translation="VSQWDNYPRKAVIPLSGRARQIYLLMAGTTNSMQSRFDNGQVIA
                     AYADGSTERLALHNPTTWWPIDQDYYIDDYAFRRPEPVPPRIHLKTGQVRILDVSDLT
                     DSLAVVPGGAATVLDLALREDKELCGLTVEALANEVIIGLMGITLIR"
     gene            484..2187
                     /gene="pelA"
                     /locus_tag="PLE88_10860"
     CDS             484..2187
                     /gene="pelA"
                     /locus_tag="PLE88_10860"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOM77007.1"
                     /translation="MKTLQAINGGTLFLKAAAVLLCLCGMGAQATVYWDRYAAKPDEW
                     FGGPEARKLADNILTWQDAHGSWPKNLDTASKPFSGSRKDIEGTFDNQATTGEMRFLA
                     RAYKATQDLRYQEAFLKALDLIIQAQYPTGGWPQSYPPGKDYSRHITFNDGAMVRLME
                     LLEDIASEPAYDFVDASRRQMCRQAFENGVQCILKCQIRVNGKLTVWCAQHDEMDYSP
                     RPARTYELASLSGGESTEILRLLMELDNPSPAVIEAIQAGVQWYESSKIEGIRIALVN
                     GQPTAVSDPQAKPMWARFYEIQTNRPFFCDRDGIPKYDYNQLGLERRKGYAWYGNWGE
                     DVFKNYQKWRQRWEHLMVAEGTKILLIIGDSTVCSWPETDARRGWGQYIQPYFMDAVK
                     VINHARSGRSTKTFIKEGLWSKALAVKPDWVLIQFGHNDSHDPNRPESTNADTDYSDY
                     LRYYIDQSRAAGAIPVLITPMHRRNFDSRGKMKDVLLPYANAMKKVANEKKVALVDLH
                     KSSGELFESLGPAGSEELANQAGDKTHFNQKGAKLMAELVMRDLPNVEPSLKKYIKEQ
                     K"
     gene            2268..2819
                     /locus_tag="PLE88_10865"
     CDS             2268..2819
                     /locus_tag="PLE88_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77008.1"
                     /translation="MSKEDMESDKIKSEELAVFWAQHQPAIAGFVCSLVPNFQDADDI
                     LQNVAVITVEKFDEYDRSKPFVGWAIGIARNLILKYYSDKKKDRITLDSKAIENISRV
                     YEAEMQTFSSHKTMVENAMERCLEQLKGRWRILVEMYYLREFSTARIAQQLGMTPGNV
                     LTSLHRVRAALRDCVANRIKREA"
     gene            2816..4540
                     /locus_tag="PLE88_10870"
     CDS             2816..4540
                     /locus_tag="PLE88_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM77009.1"
                     /translation="MMHENLDKLVSQFLYDQASMTPDQAEQLSSWISRSPDNARHFVR
                     SAIFHRCIHDYFVCSDNVRDAMLHFSADTLQFNKEASDVFNPEIWRLLSETENTAPAV
                     ECSPPTASRQPAGIQKIKVEKVPRKISRLSLATLVVSLAALLFAVVYVQVVPVAVHEE
                     VATVADTLNAQWADSDESLKSGDRLRTNTPPLLLRKGVVKLVFDNDTSVLVEAPAEFV
                     LVAGDQMNLNYGRAYAIVPRQAIGFTITTPNSKIIDLGTEFGVQVDLGQITQLHVVKG
                     KTTLVAGRESLKTTLEVNQGQAKKITSQSEVADIQCDSRLFVRDIQSKRNIIWRGGPL
                     DIADMVGGGNGLGTGKPNTGIRWEDGCFVSASDYYVGQREQGSGLFISVPDSLFIEGI
                     FVPNSENGPIALCADASLKWNLPQTRGQYCYNINNAGTISRGSGRIPFHRQILGGRPC
                     GTPDNPAVSMHANAGLTLNLEKIRQYYNGMPIVSFTALCGFSETYFEYAAKVDPELIP
                     QADFYVLVDGQERFVRKDMTPNDKPCSIYVALGPNDRYLTLATTAGSDQKVSYDWCLF
                     ACPRLNME"
     gene            4567..5310
                     /locus_tag="PLE88_10875"
     CDS             4567..5310
                     /locus_tag="PLE88_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM77010.1"
                     /translation="MKTKKGFTLIELLVVIAIIALLLSIIMPALRKAKEYAKKVICQS
                     NQHQFGVVIATYGTEFNYNFRNYKTAVGLNATQLARSWFWVNGTGDYAHEAEPRAIRF
                     IMNSGLLPDRRVFFCPSMNNLSHDKNYVAGSLVPQNTEVIYRNGQTPLFWGTFVWLWK
                     KEMREDVVSINNLSSGAMMCDMTNEAWTLGAGTNATLQNFMNNIQLKRTFQHGNVLMD
                     DMSVKNPSDKDPELVYWLWNHTKWAGRSY"
     gene            5415..7652
                     /locus_tag="PLE88_10880"
     CDS             5415..7652
                     /locus_tag="PLE88_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77011.1"
                     /translation="MMRVKHYWLVGLVCLLVVAAGASASVTDPNIISKVVRYNPDSDS
                     GDTEPNLVRGGLQTGALPYTDRTFVFGSVGRFAGIDYVQTCVDDKDNPNYRLDVTVNK
                     AGKLYLIIDNRVGSGTANDTTTPPALGNGVMDWVLAMGFTQTAYTVNISTPATVYELP
                     VAGAGTYSLGAQNDGTSRVMYLVAAAPAGWNFVPVIVGVPAAVQVEPGEKLEIDATVT
                     DDDGPSAVTVQWTTVSKPEGATVTYSPDAVSEDVEISFSDLGNYTLKLTAYDGEKTTE
                     AVIQAAVKIPEFALECTNWVEACNDTNKGPTAHYKPTSYMYVRNHSAPRRRIELISYD
                     ISSLKQPGKAIANSFISLRRRTGHSAAVLSVYGVREEFDNFNLDTGSWSNLPGLANTP
                     VPPMTDQITPASLDLADLSDLLLTYGPNVPAGSAWSNSPTSPALDAFLNADTDGTVLL
                     LFVTFTPQDADFEIFCKGYDTANSEPVTGKTGIVLRGNVMTQTWAINPSPAVNSSQTT
                     KLSQLSWTNPDGEGTISCNVYIGMGEPNALRPDYGFTTLATGITGNSVAIPAGLLAGG
                     NTYKWIVDVYDSGTGITTRGYVWSFNVLTNAAPTVQIANPIQYLWLGNAGDPASATAV
                     LDATASDDGLLMPLTYFWEQLSGPDGAEVALEPNNVEDKTLILPATGMYVFKLTVNDG
                     EHSASAAAQIFVGATPCDAAKAKPGYTPLTGDFNNDCYVDLNDFSTFALQWLECHPFM
                     DAPCR"
     gene            7791..>8022
                     /locus_tag="PLE88_10885"
     CDS             7791..>8022
                     /locus_tag="PLE88_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77012.1"
                     /translation="MNSKKCFYYAAGVLLLMQGFLQAAPYADQPKPSVGQAVDPDAVV
                     LSWQPGDGAVRSGVDLGTSQTAVAEARRPAGDI"
BASE COUNT         1952 a         1880 c         2316 g         1874 t
ORIGIN      
        1 tgtttcccag tgggataatt atccccgcaa ggcggttatt cctttgtctg gcagggcccg
       61 gcagatctat ttgctgatgg ccggcacgac gaattcgatg cagagccgct ttgataatgg
      121 gcaagtcatt gcggcatacg ccgacggctc gacagagcgg cttgcgctgc ataatccgac
      181 gacatggtgg ccgattgacc aagattatta tatagatgac tatgcattca ggcgtccgga
      241 accggtgccg ccgcggatcc atctcaagac cggtcaggta cggattctgg acgtttcgga
      301 tttgaccgac agcttggcag tcgtgcccgg cggggcggca acagtgctgg atctagcgct
      361 gcgcgaggat aaggaactgt gcgggctgac ggtagaggcg cttgccaatg aagtcatcat
      421 cggattgatg ggtataacgc tgatacgtta ggataatcaa caaaaaatga ggaaaagcag
      481 ctaatgaaaa cattgcaggc aatcaacggc ggcactcttt ttttgaaggc ggcggctgtg
      541 ctgctgtgcc tttgcgggat gggcgcacag gcaacagtgt attgggacag atatgcagcg
      601 aaaccggatg aatggttcgg cggcccagaa gcccgaaaat tagcagacaa tattcttact
      661 tggcaggacg cacacggcag ctggcctaaa aatctggata cggcttccaa gcccttttca
      721 ggcagccgga aagatatcga aggaaccttt gacaatcaag cgaccacagg ggaaatgcgt
      781 tttctggcac gtgcttacaa agcaacgcag gatttgcgtt atcaggaggc gtttctcaag
      841 gcgctggatt tgattattca ggcacagtat cccaccggcg gctggccgca gtcttatccg
      901 ccgggcaagg actactcccg ccatattacc tttaatgatg gggcaatggt gcggctgatg
      961 gagctgcttg aggacattgc ctctgagccg gcgtatgatt ttgtcgatgc ctcccgccgc
     1021 cagatgtgcc ggcaggcctt tgaaaacggc gtccaatgca ttcttaaatg ccagatacgt
     1081 gtcaatggaa aactgacggt ttggtgtgcg cagcatgacg agatggatta cagcccgcgt
     1141 ccggcgcgaa cgtatgagct ggcgtcgctg agcgggggcg aaagcacaga gattctgcgg
     1201 cttctgatgg agctggacaa tccgagccct gccgttatcg aggccattca ggccggcgta
     1261 cagtggtatg aatcatccaa aattgagggc attcgcatag cgctggtaaa cggccagccg
     1321 acggctgtca gcgacccgca ggccaaaccg atgtgggcgc gtttttatga aatccagaca
     1381 aatcggccgt tcttctgcga ccgcgacggg attccaaagt atgattacaa ccagcttggt
     1441 cttgaacgcc gtaaggggta cgcttggtat ggcaattggg gcgaagacgt ttttaaaaat
     1501 taccagaaat ggcgtcagcg atgggaacat cttatggttg ccgaagggac aaaaattctg
     1561 ctgattatag gtgattcgac tgtctgcagc tggcccgaaa cggatgcacg gcggggatgg
     1621 gggcagtata ttcagccgta ctttatggat gcggttaaag tcatcaatca tgcccgcagc
     1681 gggcgaagca ccaagacctt tatcaaagaa ggattgtgga gcaaagcact ggcggtcaaa
     1741 cccgattggg tactgattca gttcgggcac aatgattccc atgacccaaa caggccggaa
     1801 tcgacaaatg cagacacgga ttattcggat tatctgcggt actatattga ccaatcgcgt
     1861 gcggcaggag cgataccagt actgataacg ccgatgcatc gtcgaaactt tgattcccgc
     1921 gggaaaatga aggatgtcct gctgccttat gccaatgcga tgaaaaaagt cgcaaatgaa
     1981 aagaaagttg ctcttgtgga tttgcataag tccagcggag aactttttga atcgttaggt
     2041 ccggccggtt ctgaggaact ggccaatcag gccggtgaca aaacacactt caaccaaaaa
     2101 ggtgcaaaac tgatggcgga gctggttatg agggatttgc cgaatgttga gccgtcctta
     2161 aaaaagtata taaaagagca aaaatagaga caatatgaaa aaaagttatt tttttttatt
     2221 tttgtgtaag aagaaagggt gataaaagca attacagaag ggggataatg agtaaagaag
     2281 atatggagtc agataaaata aaatctgagg aattggcggt tttttgggca cagcaccagc
     2341 cggctatcgc aggatttgtc tgctcactgg ttccgaattt tcaggatgcc gatgatattc
     2401 tgcagaatgt ggcggtaatt actgtcgaga aatttgatga gtatgaccgc agcaagccgt
     2461 ttgtcgggtg ggccattggc attgcccgca atctgattct aaagtattac tcggacaaga
     2521 aaaaggacag gattacactg gattccaagg ccattgaaaa catatcgcgc gtttatgaag
     2581 ccgagatgca gacattcagc agccataaaa cgatggttga aaatgcgatg gagcggtgtc
     2641 tcgaacaact gaaaggccga tggcgtattt tggtggagat gtattatctc cgggaattct
     2701 ccaccgctcg cattgcccag cagctgggta tgacgccggg caatgttttg acctcgcttc
     2761 atcgtgtccg tgcagcattg cgggattgtg tagccaatcg gatcaagcgg gaagcatgat
     2821 gcatgaaaat ttggacaaat tggtcagcca attcctttat gatcaggcgt ccatgacgcc
     2881 ggatcaggca gagcagttgt ccagctggat cagccgcagt ccggacaatg cccgccattt
     2941 tgttcggtct gcgatatttc atcgctgcat tcacgattac tttgtctgtt cggataatgt
     3001 tcgcgacgca atgctgcatt tttcggccga tacgctgcag tttaataaag aagcttccga
     3061 tgtattcaat cctgaaatat ggcgtctttt gtcggagaca gaaaacacgg cgccggcggt
     3121 agagtgttcc ccgcccacag cttcgcggca gccggccgga attcagaaaa taaaggttga
     3181 aaaagtgccg cggaaaatca gcaggttatc gcttgcaacg ctggttgtat ctttggcggc
     3241 gctgctattt gctgttgtct atgtacaggt tgttccggtt gcggttcacg aagaagtggc
     3301 gaccgtggcc gatacgctga atgcccaatg ggccgattcg gatgaatccc tcaaaagcgg
     3361 cgatcggctg cgcaccaaca caccgccgct gctgcttcgc aagggcgtcg tgaagctggt
     3421 ctttgataat gacacaagcg tattggtgga agcgccggcg gagtttgtgt tagtagccgg
     3481 cgaccagatg aatctgaatt acggccgggc ttatgccatt gtgccgcggc aggccatcgg
     3541 ctttacgata acaacgccca attccaagat tatcgatttg gggacagaat tcggtgtgca
     3601 ggtggacttg ggccaaatca cccagctgca tgtggtcaag ggcaaaacga cgcttgtggc
     3661 gggccgggaa agccttaaga cgactctgga agtcaatcag ggacaggcca aaaaaatcac
     3721 cagccaatcg gaggttgcgg atattcaatg cgatagccgg ctgtttgtgc gggatatcca
     3781 gtccaagcga aatatcatct ggcggggcgg gccattggat attgcggata tggtcggcgg
     3841 gggcaatggt ctgggaaccg gcaagcccaa tacgggcatt cgctgggagg acggctgttt
     3901 tgtctccgca agcgattatt atgtcggtca gcgggaacag gggagcggcc tttttatctc
     3961 ggtgcctgac agtctgttta tcgagggcat ttttgtgccc aacagtgaaa acggacctat
     4021 tgccctatgt gccgatgcga gcctgaaatg gaatctgccc cagacgcgcg ggcagtattg
     4081 ctacaatatc aataacgccg gcacaatcag ccgcggtagc ggccggattc cctttcacag
     4141 gcaaatactg ggcgggcgtc cctgcggcac gccggacaat ccggctgttt cgatgcatgc
     4201 caatgccggc ctgaccctga atctggagaa aatacggcag tattataacg ggatgccgat
     4261 tgtttctttt accgcgctgt gcggattttc agaaacctat tttgagtatg cggccaaagt
     4321 ggatccggaa ttgattccgc aggcggattt ctatgtgctg gtggacggac aagaacgttt
     4381 tgtccgcaaa gatatgacac ccaatgacaa gccctgttca atatatgttg cgttgggtcc
     4441 gaatgaccgt tatctgacgc tagcgacaac cgccggcagc gaccagaaag tgtcctatga
     4501 ctggtgtctg tttgcctgtc cgcggctgaa tatggaataa cagaagaaga cagaaaggaa
     4561 attgttatga aaaccaaaaa aggttttaca ttgattgagc tgctggttgt tatagcgatt
     4621 attgcgctgc tgctgtccat tataatgccg gccttgagga aggccaagga gtatgccaag
     4681 aaggttatct gccagtcgaa tcagcatcag ttcggcgtgg tgattgccac ctatggcacc
     4741 gagtttaact acaatttcag aaattataaa accgcagttg gtctgaatgc cacccagctg
     4801 gcgcgttcgt ggttctgggt caacggcacg ggcgattatg cccacgaagc cgaaccccgc
     4861 gcgattcggt ttattatgaa cagcggtctg ctaccggacc ggcgggtctt tttctgtccg
     4921 agcatgaata atttatcaca tgacaagaat tatgtggccg gcagtctggt accgcagaat
     4981 accgaggtga tctaccgcaa tgggcagacg ccgctgttct gggggacttt tgtatggctg
     5041 tggaaaaagg agatgcggga ggatgtagtt tccatcaaca atctgtccag cggggcgatg
     5101 atgtgtgata tgaccaacga ggcctggaca ctgggggcag ggaccaatgc gacacttcag
     5161 aattttatga acaatatcca attaaaacgg actttccagc acggcaatgt gctgatggac
     5221 gatatgagcg ttaaaaatcc cagcgataag gaccctgaac tggtgtactg gctgtggaat
     5281 cacacgaagt gggctgggcg ttcgtattaa gcgggagcgg aaaagacggg aatgttcttt
     5341 ccgccgacaa gtgaataatg cgttctttcg ttgtgtgcgg aagacagaat aaactttttt
     5401 tgcggaggtc aattatgatg agagtgaaac attactggtt agtaggtctg gtatgtctgc
     5461 tggtggttgc tgcgggggcg tcggccagcg ttacagatcc gaatattatt tccaaagttg
     5521 tgcgctataa tcccgactcg gacagcggcg atacggagcc gaatctggtg cgaggggggc
     5581 tgcagacggg ggcacttccc tatacagacc gtacgtttgt ttttggaagc gtggggcggt
     5641 tcgcgggcat tgattatgtc cagacctgcg tcgatgacaa ggataatccc aattatcggc
     5701 tggatgtaac cgtcaacaaa gccggcaaac tttatctgat tattgacaac cgtgtcggca
     5761 gcggaaccgc caacgatacg acaaccccgc cggcattggg caatggggtg atggattggg
     5821 tcttggcgat gggctttacg cagacggcct atacagtcaa tatcagtacg ccggcaacag
     5881 tgtatgagct gccggttgct ggagccggca cttacagctt gggggcacag aatgacggca
     5941 ccagccgcgt gatgtatctg gtggcggctg caccggccgg atggaacttt gtgccggtga
     6001 ttgtaggagt tcctgctgca gtccaagttg agccggggga aaaattagag attgatgcaa
     6061 ccgttaccga cgatgacggt ccgtcggctg tgacggttca gtggacaacc gtttctaaac
     6121 cggaaggggc gacagtcacc tattcgccgg atgcggtcag cgaagatgtg gaaatcagtt
     6181 tttcagattt gggcaattat acgctcaagc tgacggccta tgacggggag aagacaacgg
     6241 aagccgttat tcaggctgct gtcaagattc ctgaatttgc actggagtgc accaattggg
     6301 ttgaggcctg caatgataca aataagggac cgacagcgca ttacaagccg acttcctata
     6361 tgtatgttcg caatcacagt gccccgcggc ggcggattga gcttatcagc tatgatattt
     6421 ccagcctgaa gcagcccggc aaggcgattg ccaattcgtt tatttccctg cggcgccgaa
     6481 caggtcacag tgcggctgtt ttgtctgttt acggcgttcg tgaagaattc gacaatttta
     6541 atctcgatac cggttcatgg agcaatctgc cgggccttgc caatacgccg gttccgccga
     6601 tgaccgacca gattacgccg gcatcgctgg accttgccga cctttccgat ctgctgctga
     6661 catacggccc gaatgtgccg gccggcagtg cgtggtcgaa ctcgccgaca tccccggcgt
     6721 tggatgcatt tctgaatgca gatacggacg gcactgtgct gctgctgttt gtaaccttta
     6781 ccccccaaga tgctgacttt gagattttct gcaagggtta tgatacagcc aattctgaac
     6841 ctgtaaccgg caagacgggc atcgttctgc gcggcaatgt gatgacccag acatgggcta
     6901 tcaatccgtc tccggcggtc aattccagcc agacgaccaa actcagtcag cttagctgga
     6961 cgaatcctga cggcgagggc actatttcct gcaatgtcta tatcggtatg ggtgaaccca
     7021 atgcgctgcg gccggattac ggatttacaa cactggcaac gggcataacc ggcaattcgg
     7081 ttgctattcc ggcaggactg ctggcaggcg gcaataccta caaatggatt gtggacgtct
     7141 atgacagcgg caccggcatt acaacccgcg gctatgtctg gagcttcaat gtactgacca
     7201 atgctgcgcc aacggtgcag attgcaaatc caattcagta tctctggctt ggcaatgccg
     7261 gcgacccggc ttcggcgaca gcggttcttg atgctacagc cagtgacgac gggcttctga
     7321 tgcccttgac ttacttctgg gagcagttaa gcggtccgga cggtgcagaa gttgcccttg
     7381 agcccaacaa tgtagaggat aagacgctta tcctgccggc gacaggcatg tatgtattta
     7441 agctgacggt caatgatggc gaacacagtg catcggccgc agcccagata tttgtcggtg
     7501 ctacgccgtg cgatgcggcc aaggccaaac cgggttatac gccgctgaca ggcgatttca
     7561 acaatgactg ctatgtggac ctgaatgact tcagcacatt tgccttgcag tggctggaat
     7621 gccatccctt tatggatgct ccatgcagat aacggtctga aagcacacag actttaataa
     7681 gatggcgccg ggtacttgtt ttgtgtaccc ggtgctattc ttgtatggaa ggaaatacca
     7741 gagcagcggg ggtttcaatg ttggatcaaa taatggggga ctgtcgttta atgaattcca
     7801 aaaagtgctt ctattatgcg gccggggtat tgctgctgat gcagggcttt ctgcaggcgg
     7861 caccgtatgc agaccagccc aaaccgtccg tcgggcaggc cgttgatccg gatgcggttg
     7921 tgctttcatg gcagccgggc gatggcgccg tacgcagcgg agtagatctc ggcaccagtc
     7981 aaacggccgt tgctgaagcc cgccggcctg ccggcgatat tg
//
LOCUS       DAOTBO010000112         7894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7838_length_7894_cov_5.015308, whole genome shotgun sequence.
ACCESSION   DAOTBO010000112 DAOTBO010000000
VERSION     DAOTBO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7838_length_7894_cov_5.015308"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..2008)
                     /locus_tag="PLE88_10890"
     CDS             complement(281..2008)
                     /locus_tag="PLE88_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM77013.1"
                     /translation="MNEQWYSSSMNDGKTNPVKIYHLLLRLVEGDINDAGLAELKAWF
                     QQEQAIPIYWEFIKNYTAVKLYEETSAESFGQMDLLDNSMDMDLWLALADDEKNAPEV
                     HLPACPQKPVRQWEKPAAVPKVTAGSSKLSMFSLIASLAAILFLVVYAYFVPKASRGF
                     EVATLADSINAKWADDASMKPGTRLVTGQAKSLLRGGLAKLVFDNNAVVTIEGPAEFE
                     IISEDRIKLQYGRLYSIVPQEALGFSVTTPNAMVIDLGTQFGVQVDFQGTTELHVSQG
                     KTQLIAGENKNKVKFEIFEGSARKISGISSDITDIPCNNFIFVRQINSQTNLIWRGQP
                     SISLADITGGGNGLGTGRLGVGIHPLTGEIGDIVAEDRMGEGKYITVPQNRYIDGVFV
                     PNGQFSPVVVSSKGHIFTECPVTNNIFYMEIINSKLTDPAVPMLWKEAAPGSGIYGSN
                     TCPSIFMHANLGITYNLDAIRSDFNGAEITRFTAEAGLSPSATRKGNVDVWVLVDGNV
                     RYCQKNITEKGKSYPIEIDIKKTDRFLTLITTDGGDIDYPEPARRATDSDWALFANPE
                     LVLTTGQVH"
     gene            complement(1974..2504)
                     /locus_tag="PLE88_10895"
     CDS             complement(1974..2504)
                     /locus_tag="PLE88_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77014.1"
                     /translation="MMTNSSQHARFLRMYASIQTKLYAFILSVVHNKSDADELYQETS
                     VVLWEKFSQYREECSFAAWAIGIAKNKVMQYLRENKKAKMVFTEDIYQNISDVTEKAS
                     DDIQFRIAALKICLERLKVSDRKLIAFRFHKNITVKMLSQLTGRSTDSLYKTISRIMY
                     MLKACIERTMVLQQYE"
     gene            3128..4246
                     /locus_tag="PLE88_10900"
     CDS             3128..4246
                     /locus_tag="PLE88_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HOM77015.1"
                     /translation="MMASQLLIRFLLVWLIAESIFINSARASNEVRDFMKWGSETLAC
                     IERDHRIAGQAGYYEDQSRTNPAYAWGTAILLLAYAKAAQVEQLHAQSLTELIDYLSR
                     YWVEDKGIGGYDCLPAPRNRIERYYDDNAWIAMGLMDAWTATGKPEYLKQAEKTLSFC
                     LSGIDPNDGGIWWKETWDSERRKTKNTCSAAPTAYACLRYYEATRQQQYLETAKELLK
                     WLDAHLKDSDSLYFDSIRPSTGRIGRRKWSYNSAMPLRCYVLLYKLTGDCLEQAEQIA
                     KACQKEWFDETSGAVRCESMFAFTLIEGWLELSEAAGQKHWKNLAERGMLYVRQNVRD
                     PNGRYSKRWDATNTEALTQWKLLYPAAAARAYWALAAK"
     gene            4282..5148
                     /locus_tag="PLE88_10905"
     CDS             4282..5148
                     /locus_tag="PLE88_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM77016.1"
                     /translation="MNSKKAFTLIELLVVIAIIALLLSVIIPALKKVKKQAAAVVCLS
                     NMRSMATSWHSYAMDHDSKLVNGHVPRRIFASPCFWVEAPQDRNGVYTGEKALSNTVL
                     PVQEEQNGIRKGLLFPYLEAVDAYHCPADKSALLFSDRSTAGYGSWWNSYSITGMMNG
                     EASKETYLLYNPSATSGWDNHSVFKLSEISSPSSRIVFLENADWRGWLMGSWLMNYTT
                     PYWTDPFAIWHGTTSPFSPLGFADGHAENHRWVNKSTWDMAQKHLFNVTPPANEREDI
                     EFMRLAYLPRGK"
     gene            5242..7545
                     /locus_tag="PLE88_10910"
     CDS             5242..7545
                     /locus_tag="PLE88_10910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOM77017.1"
                     /translation="MKCRWNVATAWMLFICCLSPLGYATQPSSFSYADYVNPLVGTDS
                     TFNFSTGNTYPAVAVPRGMNFWTPQTNKMGDGWCYQYGARKIRGFKQTHQPSPWINDY
                     AAFSLMPIVGQLTVDEEKRASWFSHKAETARPYYYRVYLADYDVTAEITPTERAAQFR
                     FTFPESDSSYILLDGFDKGSYVKILPAERTIIGYCRNNNGGVPQNFHNYFIVVFDRDF
                     EEVYTWNNGRVQSGQTEEQGNHVGAAVRFRTHKGQQIQIKAASSFISPQQAKLNLQRE
                     LADDSFDQTCRKAKDIWNSHLGRFRVSGGTEAQYQNFYTALYRALLFPRMFYELDAAG
                     RIVHYSPYNGQVLPGYMFTDNGFWDTFRAVFPFLTLMYPELNAQIMEGLVNTYTESGW
                     LPEWASPGHRDCMIGSNSAVNIADAYLKGIRGYDIDTLYEAILKNSENAGPLRSVGRF
                     GVEYYNTLGYIPCDVNVNESVARTLEYCYADFTIWKLARALNRPQDEIDRFFRRAQYY
                     KNVFDASTNFMRGRRKDGSWSEPFMPEKWGGDFTEGCAWHYTWSVFHDPQGLIDLMGG
                     REAFAAKLDAVFTTPPKYDCSYYGFPIHEIIEMTVMDMGQYAHGNQPIQHMIYLYNYA
                     GQPWKAQKWVREVMNRLYTPTPDGLCGDEDNGQTSAWYVFSALGFYPVCPGSGQYVLG
                     APLFKNAVLHLPNGKIFEISAPKNSAENLYIQSAKLNGRTYTKTYLQHHDIIKGGKLQ
                     IAMGDKPNPNWGSGPDDVPYSMSSEKH"
     gene            7602..>7894
                     /locus_tag="PLE88_10915"
     CDS             7602..>7894
                     /locus_tag="PLE88_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77018.1"
                     /translation="MGMKTLPILLAFFVMGVADAMGPMSDAVRQNYQLSNVMATLLSF
                     FVFIAFAVFSVPGGLLATYIGKKKLLLLGLGLNAAAMLIPCLMQPGFALLLVC"
BASE COUNT         2088 a         1857 c         1851 g         2098 t
ORIGIN      
        1 gccaacatca caacatactt cttcatcttt ccttacctcc aaaaaagtta aagtttacac
       61 aaaacacact tctttaaaat ccttttcctc ttctgccatc cataggatac cgtttttctg
      121 cagctttcat atttgggtat tttgtctaat tctgccaacc tcctttcttt ctgcataata
      181 tctgtcatcc ctcttttatg cagttctctt ttgtctgaac gaagtctttc atacatacca
      241 ttatatcaca agtgttttcc aatttaccca ttttctcgat ttaatgaact tgtccagtcg
      301 taagaaccaa ttccggattg gcaaacagtg cccagtccga atctgtggcc cgtcttgccg
      361 gttccggata atctatatct ccgccatcgg ttgtaattaa tgtcaaaaag cggtccgttt
      421 tcttgatgtc aatctcgatg ggataagatt tccctttttc ggtaatattt ttctgacaat
      481 accgcacatt accgtcaacc agcacccaga catccacatt tcccttacgc gttgcactgg
      541 gagacaggcc cgcttcagcg gtaaaacggg taatttcagc accattaaaa tcagaacgaa
      601 ttgcatccag attatatgta atccctaaat ttgcatgcat gaagatgctt gggcaggtat
      661 tggagccgta tatgccgctg ccgggtgctg cttctttcca cagcatcggg acagcagggt
      721 cggttagttt gctgtttata atctccatat agaaaatatt attggtcact gggcactcgg
      781 taaaaatatg ccctttggaa cttactacta caggggaaaa ttgtccgttg gggacaaaaa
      841 caccgtcgat ataacggttt tggggaacgg ttatatattt cccttcaccc atacgatcct
      901 cggcaacaat atcgccgatt tcaccggtta gaggatgtat gccgacaccc agtcggccgg
      961 ttccaagccc atttcccccg cctgtaatat ctgccaaact gattgacggc tgacctcgcc
     1021 aaattagatt ggtctgggaa ttaatctgac gaacaaagat gaaattattg cagggaatat
     1081 ctgtaatatc agacgaaata cccgagattt tgcgtgctga accctcaaaa atttcaaact
     1141 taaccttgtt tttgttttct ccggctatta attgggtttt gccttggctg acatggagtt
     1201 cggttgtacc ctgaaaatcc acctgaacac cgaattgggt acctaagtcg ataaccatcg
     1261 cattgggcgt agttacagaa aagcccagtg cttcctgcgg gacaatagaa taaagccggc
     1321 cgtactggag tttgatccgg tcttcggaaa taatctcgaa ttcggctggg ccttcaatgg
     1381 ttacaacagc attgttgtca aataccagtt ttgccagacc gccccgcaat aaggattttg
     1441 cctgtccggt aacaagccgt gttccgggct tcatagaagc atcatcagcc catttggcat
     1501 tgatgctgtc agccaatgta gccacttcaa aacctcggct cgctttgggg acaaaatagg
     1561 catacacaac aagaaataga attgcagcca aggaggcaat cagactaaac atggaaagtt
     1621 tgctgcttcc ggctgtcacc ttggggacgg cggcaggttt ttcccactgc ctgactggct
     1681 tttgcggaca agcagggaga tgtacttccg gtgcgttctt ttcatcatcg gccaaagcca
     1741 gccataaatc catatccatt gaattgtcaa gcaaatccat ctgcccgaag gattctgcgg
     1801 aagtctcctc atacagctta acggccgtat agtttttgat aaactcccaa tagatcggaa
     1861 tggcttgctc ctgctgaaac cacgctttca gctcagcaag cccggcatcg ttgatgtccc
     1921 cttccaccag acgcagaagc agatggtaaa tcttgactgg atttgtcttg ccgtcattca
     1981 tactgctgga gtaccattgt tcgttcaata catgctttaa gcatatacat tattcgggag
     2041 atagttttat acagcgagtc cgttgaacgt cctgtcagct gggagagcat tttgactgta
     2101 atatttttat gaaatcggaa tgctatcagc tttcgatcag atactttcag ccgctccaga
     2161 catattttta atgcggctat gcggaactga atatcatctg acgctttttc tgttacatct
     2221 gatatattct ggtatatatc ctccgtaaat accatttttg cctttttgtt ttcccgcagg
     2281 tattgcataa ccttattctt ggcaattcct atagcccaag cggcaaagga gcattcctcc
     2341 cgatattggc tgaatttttc ccataaaaca acactggttt cctgatacaa ttcatcggca
     2401 tcactcttat tgtggacaac ggacaaaatg aatgcataca gtttagtttg tatgctggca
     2461 tacatccgca ggaaacgtgc atgttgagag gaatttgtca tcatcttttt ctatgtttac
     2521 aatttatcgt ctaatattta cttgaaatgc agtgtaaaaa ttggaaggaa aaaataggaa
     2581 aaataataaa agttttttag tctcaacaaa ccaatatcag actataccaa cacaatatta
     2641 cttgtattat gttttattaa agctgcatcg atgagtatat tgatttaact atttcccgaa
     2701 tatttacatc tgattttaat agaatttgtt ttatagatat catttatttt gactttttct
     2761 aaaaaaaatg caaaaaataa ccttcaaaaa ccgcctcatc cgtgaaaata taaataagca
     2821 gttttcataa ataactggtt aaaaaggatc acggaagaaa ggcaggctat gccggcagaa
     2881 agagccagca aacaagtttt tctgtgcagt agcatttttg cccatcatgc gtccatttta
     2941 tcaggtgaaa acagccgcca tacgattcag ttgtgcactc aatcgtttac aagaacaaga
     3001 catttgagat gtaagaaatg ccaattaggc ggatatactg gtattcgcca gatataagca
     3061 tactgacccc gcaaggatct tggatgactc gaatacattt tgcagggaca cgtacaaaag
     3121 ggagtaaatg atggcatcac agctgcttat tcgattcctt ctggtttggc ttattgcgga
     3181 atcgattttt atcaacagcg cccgggcatc gaatgaagta cgcgatttta tgaaatgggg
     3241 ttctgaaacg cttgcctgca ttgagcggga ccatcggatt gccggtcagg caggctatta
     3301 cgaagaccag agccggacca atccggcata tgcatgggga acggccattc tgttactggc
     3361 ctatgccaag gccgcccaag tcgaacaact ccatgcacaa tctttaaccg aactgattga
     3421 ctatttaagc agatactggg tggaagataa gggcattggc ggatatgact gcctgccggc
     3481 tcctcgaaac aggatcgagc gatattacga cgacaatgcc tggatcgcaa tgggactgat
     3541 ggatgcttgg actgccaccg gtaaaccgga gtatctcaaa caggccgaaa aaacactgtc
     3601 tttttgctta agcggaatag atcccaacga cggcggcatt tggtggaagg agacatggga
     3661 cagcgaacgc cgcaaaacca aaaacacctg ctcggctgca ccaacggctt atgcgtgtct
     3721 gcgttattat gaggcaaccc gccagcagca gtatcttgaa accgccaaag agcttttaaa
     3781 atggctggat gcgcatttaa aagacagtga ttccctgtat tttgacagca tccgcccctc
     3841 aacaggacga attggacggc ggaaatggtc gtataattca gctatgccgc ttcgctgtta
     3901 tgtcctgctg tataagctga ccggcgattg tttggaacag gccgaacaaa tagccaaggc
     3961 ctgccagaaa gaatggtttg atgagaccag cggcgctgtt cgatgcgagt cgatgtttgc
     4021 ctttacactt atcgagggct ggcttgagct gtctgaagca gcaggccaga aacactggaa
     4081 aaaccttgcc gaacgcggta tgctgtatgt tcggcagaat gtgcgggatc ccaacggccg
     4141 atactccaag cgatgggatg ctaccaatac agaagcactg actcaatgga aactgctgta
     4201 tccggcagca gcagcccgag cttattgggc tttggctgcg aaataaaatt acaataacct
     4261 tcaagaacag gagtcatacc gatgaactcc aaaaaagcat ttacgctgat cgagctgctg
     4321 gtagtcattg ccatcattgc tctgctgctt tcggtcatta ttccggcact caaaaaagtc
     4381 aaaaaacagg ccgctgctgt cgtatgcctg tcgaatatga gaagcatggc aacatcgtgg
     4441 cattcctatg caatggacca tgacagcaaa ctggtcaacg gccatgtgcc ccgccggatt
     4501 tttgcgtctc cttgtttttg ggtcgaagcc ccgcaggacc gcaacggggt ttataccggc
     4561 gaaaaagccc tcagcaatac agttctgccg gttcaagaag aacaaaacgg catacgaaaa
     4621 gggcttttat tcccctacct tgaagcggtg gatgcctatc actgccctgc tgacaaaagt
     4681 gcattgctgt tttccgacag gtctaccgcc ggctatggct cttggtggaa cagctattct
     4741 attaccggca tgatgaatgg ggaagcatca aaagagacat acctgctcta caatccttct
     4801 gctacaagcg gatgggacaa ccactctgta ttcaaactgt cggaaatatc aagtccctca
     4861 agccgcatcg tttttttgga aaatgcggat tggcgcggct ggctgatggg atcgtggctg
     4921 atgaattata cgacacctta ctggaccgat ccctttgcca tctggcacgg gacaacaagc
     4981 cctttcagcc cgctgggatt tgcagatgga catgccgaaa atcaccggtg ggttaacaaa
     5041 tcgacatggg atatggctca aaaacatctt ttcaatgtca ccccgccggc caatgaacgg
     5101 gaagatattg aatttatgcg gcttgcgtat ctgccccggg ggaaataatg cctggcggct
     5161 gcagaataca atacaatccg aatccgcaga caagcaaatg cagtgttgaa taataactat
     5221 tggacaatca gggaggcctt tatgaagtgc agatggaacg ttgcgaccgc atggatgctc
     5281 tttatctgct gtctgtcacc gctgggttat gccacacagc cgtcttcgtt ttcttatgca
     5341 gattatgtaa atccgctcgt gggaacggat tcgactttta acttttcaac gggaaacacg
     5401 tatcctgcag tggcagttcc ccgcggaatg aacttctgga cgccgcagac caacaaaatg
     5461 ggagacggct ggtgctacca gtacggtgcc cgcaaaatcc gcggcttcaa acagacccat
     5521 caaccgagtc catggatcaa tgattatgcg gcattttctc tgatgccgat tgtcggtcaa
     5581 ttgacggtcg atgaggagaa acgggcatcg tggttttccc ataaggccga aacagccaga
     5641 ccttattatt accgcgtgta tttggcggat tacgatgtaa cggcggaaat aaccccgaca
     5701 gaacgggctg ctcagtttcg attcaccttc ccggaatcgg attcttccta catcctgctg
     5761 gacggttttg ataaagggtc gtatgtcaag atactgccgg ccgagcggac catcatcgga
     5821 tattgtcgaa ataacaacgg cggggttccg cagaactttc ataactattt tattgtcgtc
     5881 tttgacagag attttgagga ggtctataca tggaataacg gcagggttca aagcgggcaa
     5941 accgaagagc agggaaatca tgtcggtgcg gccgtgcgtt tccgcacaca caaaggccag
     6001 cagatccaaa tcaaagcggc ttcctctttt atcagtccgc agcaggccaa actgaatctg
     6061 cagcgggaac tggccgatga ttcttttgac caaacatgcc gtaaagcaaa agatatatgg
     6121 aacagccatt tggggcggtt ccgcgtcagc ggaggcaccg aagcacaata tcagaatttt
     6181 tatacagcct tgtaccgggc gctgctgttt ccccggatgt tttacgaatt ggatgcagcg
     6241 ggcagaattg tgcattacag tccctacaac ggtcaggttc tgccggggta tatgttcacc
     6301 gacaacgggt tctgggatac ctttcgtgcg gtttttccat tcctgacgct gatgtatcct
     6361 gaactcaatg cccagattat ggaaggtctg gttaatacct atacagaaag cggctggctt
     6421 ccggagtggg ccagtccggg acatcgggac tgcatgatcg gctccaattc agccgtcaat
     6481 atcgccgatg cgtatctgaa gggcattcgg ggctatgaca tcgataccct gtacgaagca
     6541 attctgaaga attccgaaaa tgccggtccc ctccgttccg tcggacggtt tggtgtcgaa
     6601 tattataaca cgctggggta tatcccctgc gatgtgaacg tcaatgaaag cgttgcccgg
     6661 acgctggaat actgttatgc ggattttacc atctggaagc tggccagagc gctgaatcgc
     6721 ccgcaggatg agattgaccg cttttttcgc cgggcacagt actacaaaaa tgtttttgat
     6781 gcctcgacca attttatgcg cgggcgaaga aaagacggct cctggtccga accttttatg
     6841 ccggaaaaat ggggcggaga tttcaccgag ggatgtgcat ggcattacac gtggtctgtt
     6901 tttcacgacc cgcaggggct gatagattta atgggcgggc gtgaagcatt tgcagccaaa
     6961 ctggatgctg tttttacaac tccccccaag tatgactgtt cgtactacgg tttccccatt
     7021 catgaaatta ttgagatgac ggtaatggat atgggccagt atgctcacgg caatcagccg
     7081 attcagcata tgatttacct gtacaactac gccggccagc cgtggaaagc gcagaaatgg
     7141 gtacgggaag taatgaacag actctatacg cctactccgg acggcttatg cggggatgaa
     7201 gacaacggcc agacctcagc ttggtatgta ttttcggcac tcggctttta tccggtctgt
     7261 cccggttcgg gccagtatgt gctgggagct ccgctgttca aaaatgcggt gcttcacctg
     7321 ccgaatggaa aaatcttcga aatttcggct cctaagaatt cggcagaaaa cctctatatc
     7381 caatcggcta aactgaatgg acgaacgtat acaaaaacct atctgcagca tcacgatata
     7441 atcaagggcg gcaaactgca gattgcgatg ggagacaagc ccaatccaaa ctggggcagc
     7501 ggcccggatg atgtcccgta ctcgatgtcc agcgaaaaac attgaacgtt atcttggtca
     7561 gatatgctgt taattagtca cctctatcga aaggaaattt tatgggaatg aaaacactgc
     7621 ctattctgct ggcctttttt gtaatgggag ttgccgatgc gatggggcct atgtcagatg
     7681 ccgttcggca aaactatcag ctgagcaatg tgatggccac cctgctgtcc ttttttgttt
     7741 ttattgcctt tgcggtattc agtgtgccgg gggggctgtt ggcaacttat atcggcaaga
     7801 aaaaactgct tcttctgggg ctgggattga atgctgcggc catgctgatt ccctgtctga
     7861 tgcagccggg atttgcgctg ctgctggtct gcat
//
LOCUS       DAOTBO010000113         7792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_7976_length_7792_cov_5.681789, whole genome shotgun sequence.
ACCESSION   DAOTBO010000113 DAOTBO010000000
VERSION     DAOTBO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7976_length_7792_cov_5.681789"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLE88_10920"
     CDS             <1..400
                     /locus_tag="PLE88_10920"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77019.1"
                     /translation="RRVRHPVYTASLILDVPSEEGISAGLVLFMNERHHYFLAVQRSG
                     GNARLYLECVNRGQAGRLAEKELPLPNGSIGLQADVQKAVCSFRYTLADDRWETLMDN
                     ADASLISFSVPDALFLGATVGPYARMDEAK"
     gene            474..2036
                     /locus_tag="PLE88_10925"
     CDS             474..2036
                     /locus_tag="PLE88_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOM77020.1"
                     /translation="MYRLIVWACMYCLTIGSVLAEPAQNPIIWADVPDVAVMRVGDTY
                     YMSSTTMHMSPGLPIMKSNDLVHWNLVGYAYDRLVENDAMNLENGRNCYGRGTWASSL
                     RFHNGTYYASTFSSTSGRTHIYSTKDIEKGPWTETSFAPSLHDHSLFFDDDGRVYMIY
                     GVGNLRLVELKPDLSGIKEGGFDEVVIPNASAVAGPAIGLPAEGSQMLKVNGKYYVMN
                     ITWPRGGMRTQIVHRADTITGPYEGRVVLQDKGVAQGCLIDTPDGKWYAVLFQDSGSV
                     GRCPWLVPVRWEDGWPVLGIDGKAPQTIDIEDNRRGLGNIVASDEFDRQPGQPLPLAW
                     QWNHNPDNRLWSVGQRSGWLRLTTGRVDSDVLQARNTLTQRTFGPVCAASTKLDTEGM
                     KDGDSAGLIALQRKYGFVGVKMDGPKKLIVMVSAQSDKPEEIESIPLEQQMVYLKIEC
                     DFRNRADKAYFYYSLDGSSWTRIGSVLQMAYTMPHFMGYRFGLFNMASKTPGGYADFD
                     YYHIDDAIAAGR"
     gene            2129..2995
                     /locus_tag="PLE88_10930"
     CDS             2129..2995
                     /locus_tag="PLE88_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOM77021.1"
                     /translation="MKSTNRFLWIVFLLVTAASQADTDPNFYIFLCFGQSNMDGAAQI
                     EQQDQTAPERFWVLAALDNPAMGREKGNWYPAVPPLCRSRAGLGPADYFGRTLAAHLP
                     EPIRVGVLVVAVPGCKIELFEKDTYQTYVQTAPPWMTGYIKDYNGNPYQYLVDMAKIA
                     QQAGVIKGILLHQGESNTGDRDWPKKVKGIYDNLIKDLNLRPEDVPLLAGETVHADQQ
                     GVCAGANQIIQELPKHLANAYVISSAGCTCKPDRLHFDSAGYRQLGKRYAEKMLSLLG
                     YEAAASSATDKQ"
     gene            3043..4710
                     /locus_tag="PLE88_10935"
     CDS             3043..4710
                     /locus_tag="PLE88_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM77022.1"
                     /translation="MVWQKAVFGVIAGWMVSAVSAYGQPPSPAADRVAVDTSKEPIAS
                     GPFEPTWESLRQYECPEWFRDAKFGIWAHWGPQCQPQRGDWYARQMYAEDHWQGKYHR
                     ENYGHPSKVGFKDVIHAWKAENWDPDRLMTLYKRAGAQYFMAMANHHDNFDMWDSTYQ
                     PWNAVNMGPQKNIIAGWAAAARRQGLRFGVSLHAAHAWSWYEIAQSSDKKGPMAGIPY
                     DGTLTKADGQGQWWEGYDPQDLYAQNHKPSRDFENLAAIHSQWDWGNGASLPDQAYCD
                     KFYNRTADLINKYQPDLVYFDDTGLPLYPVSDAGLKIAAHLYNSSAARSGGSNQAVLF
                     GKVLTPEQRECLTWDVERGVCNDIQPQPWQTDTCLGTWHYDIGIYERGRYKTAKTVIQ
                     MLVDIVSKNGNLLLSVPVRADGTIDDKEQQILEDIAAWMDIHKECIFGTRPWRIFGQG
                     PALEGTAPITAQGFNEGRGKPLTAQDIRFTAKDGILYAVAMGWPQETLTISSLGTDQA
                     SGQAIDRIDVLGSQEIVQWEQTREALLIKPLKNKVCDETTAFRITFR"
     gene            4777..7302
                     /locus_tag="PLE88_10940"
     CDS             4777..7302
                     /locus_tag="PLE88_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOM77023.1"
                     /translation="MNRKLIVLAVAVWICSLQAAAAVEDFKPASTNQPGRQYPQVNSE
                     RRVRARIAAPEAQNVLLDIGGVKYPMTKGQDGVWIGDSEPQDEGFHYYQLVIDGAQVP
                     DPGSMFFYGASRWGSGVEVPAADEAFYALQNVPHGQVRQHLYFSKITNAWRRCFIYTP
                     PAYDGNPSIRYPVLYLQHGGGEDETGWSNQGKANLIMDNLIAQGKARPFIIVMDNGTW
                     TTPGQPRPRRGEGGTWPPAGWADTFKRVLLEEIIPMIDAEFRTLADQPHRAMAGLSMG
                     GMQTNAIAMENLDVFSYIGIFSGGTVGDPATAHNGVMANAAEFNKKVKLVFESCGSRE
                     NPDAIRSHVQQLKDAGINAISYVSPDTAHEWQTWRRSLYQFAQLLFQDLPLPSPAPAP
                     AQQPQAPAAAAVIRINAGAFTPYTDSKGQVWKPDEGFQGGSTIDRDPDLAIVGTEDRA
                     LFLTEHYAMDAFSCKVPNGRYIAKLYFAETFEGIYGPGERVFSFNVQGKEFKDFDIWV
                     KAGGPNRAYIETVPVEVTNGEFRITFTFQQENPAINAIELIPQPADSASGAAAAPAAQ
                     ARPDRPRGGFGGPIELGPDDKAVFPDPPADFRSQRDNIARGSEEMVEYDSKTVGTRRK
                     MLIYTPPGYSTDRRYPVLYLLHGIGGDETEWQRFCSPNVILDNLIADGKAEPMIIVMP
                     NGRAQKNDRAEGNVYNTAPAFANFEYDLLNDVIPAVEARYSVYKDRQHRALAGLSMGG
                     GQSLNFGLGHLDTFAWIGGFSSAPNTKAPAELIPDPAAAKEQLKLLYISCGNKDGLIR
                     ISQGLHNYLKEHNIPHIWNVDSHGHDPDSWGKNLYHFAQHLFK"
     gene            7319..>7792
                     /locus_tag="PLE88_10945"
     CDS             7319..>7792
                     /locus_tag="PLE88_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOM77024.1"
                     /translation="MKIGKWQSIVLLLSIVFCPAARAADSGRPALKEVFKEYFLIGGA
                     LNRNVVTGRDPNAAAIAIAHFNTATPENDMKWALIHPEPGQYNWEPADRFVEFCQQHQ
                     MIPIGHCLVWHSQVPRWTFQDDAGNPLTREALLERMKEHITAVVRRYKGRIRGWDV"
BASE COUNT         1798 a         2087 c         2231 g         1676 t
ORIGIN      
        1 ccgccgcgtc cgccaccctg tgtataccgc ctcgctaatt ctcgatgtgc ccagcgagga
       61 ggggatttcc gccggcttgg tcctgtttat gaatgagcgg caccattatt ttctggctgt
      121 tcagcgcagc ggcggcaatg cccgcctgta tctggaatgc gtcaatcgcg gccaagcagg
      181 ccgattggcg gaaaaagagc tgcccttgcc aaacggcagc atcggattgc aggccgatgt
      241 ccagaaggcc gtctgttcgt tccgatatac gcttgccgat gaccgctggg aaaccctgat
      301 ggacaatgcc gatgcgtcgc tgatttcgtt ttctgttccg gatgcgctct ttctgggcgc
      361 tacagttggc ccgtatgcac gcatggatga agccaaataa aaactggctg gacggccgga
      421 ctgccgttat aataatgaaa tcttcagtca ttgttttaag gagatttagc aaaatgtatc
      481 gattgattgt atgggcctgc atgtattgtt taacgattgg ttccgttctg gccgaaccgg
      541 cgcagaatcc tatcatctgg gctgatgtgc cggatgtagc cgtcatgcgg gtcggcgata
      601 cttattatat gagcagcacc acaatgcata tgagtcccgg cctgccgatt atgaaatcca
      661 acgatctggt ccattggaat ctggtcggct atgcttatga ccggctggtt gaaaatgatg
      721 cgatgaatct ggaaaacgga cgcaactgct acggccgggg cacgtgggcc agcagcctgc
      781 gttttcacaa cggcacctat tatgcatcaa ccttttccag caccagcggc cgaacccata
      841 tttacagcac caaggatatc gaaaaaggcc cgtggacgga aacatccttt gccccttccc
      901 tgcatgacca ttccttgttt tttgatgatg acggccgcgt gtatatgatt tacggcgtgg
      961 gcaatcttcg gcttgtggaa ctcaaaccgg atttgtcggg catcaaggaa gggggctttg
     1021 atgaagtggt catccccaat gccagcgctg tggccggacc cgctatcggc ctgccggccg
     1081 aaggctcaca gatgctgaag gtcaacggca aatattatgt catgaacatt acctggccgc
     1141 gcggcggaat gcgcacgcag attgttcatc gagccgatac aatcaccggt ccctatgaag
     1201 gccgtgttgt gctgcaggat aagggcgttg cgcagggctg tctgattgat acgccggacg
     1261 gcaaatggta tgcggttttg tttcaggaca gcggttcggt cggacgctgt ccgtggctgg
     1321 tgcctgtccg atgggaagac ggctggccgg tattgggaat cgacggcaaa gcgcctcaga
     1381 ccattgatat agaagacaat cgccgcggtc tgggcaatat tgttgcctcg gatgagtttg
     1441 accgccagcc gggtcagccg ctgcctctgg cgtggcagtg gaatcacaat cccgacaacc
     1501 gcctctggtc tgtcggccag cggagcggct ggctgcggct gacgaccggc cgcgtggaca
     1561 gcgatgtgct gcaggcacgc aatacgctga cccagcggac cttcggcccc gtctgtgcgg
     1621 ccagcaccaa actggatact gagggcatga aagacggcga ttctgcaggc ctgattgcct
     1681 tgcagcggaa gtacggattt gtcggggtaa aaatggacgg gccgaagaaa ttgatcgtta
     1741 tggtcagcgc ccagtccgat aagccggaag aaatcgaaag catcccgctg gaacagcaga
     1801 tggtttatct gaaaattgaa tgcgatttca ggaaccgcgc cgacaaggcc tatttctatt
     1861 acagtcttga cggcagcagc tggacgcgaa ttggttcggt tctgcagatg gcctacacca
     1921 tgccgcactt tatgggctac cgtttcggtt tatttaacat ggcctccaaa acgccgggcg
     1981 gctatgcaga ttttgattat tatcatattg atgatgccat tgccgcaggc cggtagattc
     2041 tgcgggtatt ttgccggctg acagagatat ccggccggac gaaaggaaga atgctgatgc
     2101 gatttcataa caattgagga tttgaaaaat gaaaagcaca aacaggtttt tatggatagt
     2161 gtttcttctt gtgacggcgg cttcccaagc cgataccgat ccgaattttt acattttcct
     2221 ctgctttggg cagtccaata tggacggcgc agcgcagatt gaacagcagg accagacggc
     2281 ccccgaacgg ttttgggttt tggcagcgct ggataatccg gcgatggggc gagaaaaagg
     2341 aaactggtat cctgctgttc ctcccctgtg ccgaagccgg gccgggctgg gtcctgccga
     2401 ttatttcggg cggacgctgg cggcccatct gcctgagcct atccgggtgg gcgtgcttgt
     2461 cgttgcggtt cccggctgca agatagaatt gtttgaaaaa gacacgtacc aaacctatgt
     2521 tcaaacagcg ccgccgtgga tgaccgggta tatcaaggac tataacggca atccctatca
     2581 gtatctggtc gatatggcca agattgccca gcaggccggg gtcatcaaag gcattctcct
     2641 gcatcagggc gaatcgaata ccggcgacag ggactggcct aagaaagtca aaggcattta
     2701 cgacaatctc atcaaggacc tcaatcttcg tcccgaagat gtgccgctgc tggcgggaga
     2761 aacggtacat gccgaccagc aaggggtttg tgcaggtgcc aatcagatta ttcaggaact
     2821 gcccaagcat cttgccaatg cctatgtcat ttcgtcggcc ggctgcacct gcaagccgga
     2881 ccgtctgcat tttgattcgg ccggctacag acagctggga aagcgctacg cagagaaaat
     2941 gctttctttg ctgggctatg aagcagcagc atcctctgca acggacaaac aatagccctg
     3001 tcaggcaaac tttgtaggca ggattgaaga aaggacgctc aaatggtttg gcaaaaagcg
     3061 gtttttggag tcatcgccgg atggatggtg tcggctgtca gcgcgtatgg acagccgccc
     3121 agccctgcgg cagatagggt ggctgtggat acatcaaaag agcccatcgc ctccggtccg
     3181 tttgagccga cgtgggagtc tctcagacaa tacgaatgcc ccgagtggtt tcgggatgcc
     3241 aagttcggca tctgggcaca ctggggacct caatgccagc cccagcgggg cgactggtac
     3301 gcccggcaga tgtacgccga agaccattgg cagggcaaat atcatcggga aaactacgga
     3361 catccgtcga aggtcggctt taaggatgta attcatgcct ggaaagccga gaactgggac
     3421 cctgatcggc tgatgacact gtataagcgg gccggtgccc agtattttat ggcgatggcc
     3481 aaccaccatg acaactttga tatgtgggac agcacctacc agccttggaa tgccgtgaat
     3541 atgggccctc aaaaaaacat catcgccggc tgggccgcag ctgcccgccg gcagggactg
     3601 cgcttcggcg tcagtctcca tgccgcacac gcctggagtt ggtatgaaat cgcccaaagt
     3661 tccgacaaga aaggccccat ggcgggcatc ccgtatgacg gcactttaac aaaggcagac
     3721 ggacaagggc aatggtggga ggggtacgat ccgcaggatt tatatgccca gaaccataag
     3781 cccagtcggg attttgaaaa tctggcagcc atccattccc agtgggactg gggcaacggc
     3841 gccagtctgc ccgatcaggc ctattgcgac aaattctaca atcgaacggc ggatttgatc
     3901 aacaagtatc agccggattt ggtgtacttc gacgatacag gactgccctt gtatccggtc
     3961 agcgacgctg gtttgaaaat tgccgctcat ttgtacaaca gcagcgccgc acgcagcggc
     4021 ggaagcaatc aggcggtgct gttcggcaaa gtgctcaccc ctgagcagcg cgagtgcctg
     4081 acgtgggatg tggaacgcgg cgtatgcaat gatattcagc cccagccatg gcagaccgat
     4141 acctgtctgg ggacatggca ttacgacatc ggcatttatg agcgcggccg gtataagaca
     4201 gccaaaaccg tgatacagat gctggtggat atcgtcagca aaaacggcaa ccttctgctc
     4261 agtgttccgg tgcgcgccga tggaacaatt gatgacaaag aacagcagat tctcgaagac
     4321 attgccgcct ggatggacat tcataaagaa tgcattttcg gcacccgtcc atggaggatc
     4381 tttgggcaag gtccggcatt ggagggtaca gcgccgatta cagcacaggg atttaacgaa
     4441 ggacgcggca agccgctgac agcgcaggat attcgcttta ccgccaaaga cgggattctt
     4501 tatgctgttg caatgggctg gcctcaggaa acattgacaa tcagctcgct tggaacagac
     4561 caagcgtccg gtcaggccat cgaccggatt gacgtgctcg gaagtcagga aatcgttcag
     4621 tgggaacaaa cccgagaagc cctgctgata aaacccctta aaaacaaggt ctgtgatgaa
     4681 accaccgctt ttcgaatcac cttcagatga acatagaact gcataaacgt atgctcgaac
     4741 aaaagttagc aagcaacaag aacaggaggc aattgtatga acaggaagtt gatagtgtta
     4801 gcagtggcag tatggatctg cagtctgcaa gcggcggcgg ccgttgagga tttcaagccg
     4861 gcctcaacca atcagccggg gcggcagtat ccgcaggtta actccgaacg ccgcgtaagg
     4921 gcgcggatcg ccgcccccga agcccagaac gttcttctgg atattggggg cgtcaaatac
     4981 cccatgacga aaggccaaga cggtgtctgg atcggtgatt cagagccgca ggatgagggc
     5041 ttccactatt atcagcttgt tatcgacgga gcgcaggttc cggatcccgg cagcatgttc
     5101 ttctacggcg ccagccggtg gggcagcggc gtcgaggtcc cggcggccga tgaggctttc
     5161 tatgcccttc aaaatgttcc ccacggtcag gtgcgtcagc atctgtattt ttcaaaaatc
     5221 accaatgcct ggcggcgctg ctttatctat actccgccgg cctatgacgg gaatccctct
     5281 atccgctatc ccgtattgta cctccagcat ggcggcgggg aggatgagac cggctggtcc
     5341 aatcagggca aggccaatct gattatggat aacctgattg cccaaggcaa ggccagaccg
     5401 tttatcatcg tgatggataa cgggacctgg acgacgccgg ggcagccccg accccgccga
     5461 ggggaaggcg ggacatggcc gccggcaggc tgggccgaca cctttaagcg tgttcttctg
     5521 gaagaaataa tcccgatgat tgatgccgag ttccgcacac tggccgacca gccgcaccgg
     5581 gctatggccg gtttgtcgat ggggggaatg cagaccaatg ccatcgccat ggaaaatctg
     5641 gatgtctttt cctatatcgg catcttcagc ggcggcacgg tcggggaccc ggccactgca
     5701 cacaacggcg ttatggccaa tgcggctgaa ttcaacaaaa aagtaaagct tgtttttgaa
     5761 agctgcggct cgcgggaaaa tcccgatgcc attcgttctc atgtccagca gctcaaggac
     5821 gccggaatca atgccatctc ctatgtttca cccgatacgg cccatgaatg gcagacgtgg
     5881 cggcgcagtc tgtatcagtt tgcccagctg ctgttccagg acctgcccct tccttcgcct
     5941 gcaccggcgc ctgcgcagca gccccaagct cctgcggctg cggcggtgat ccgcatcaat
     6001 gccggcgcgt ttacccccta tacggactcg aaaggacagg tctggaagcc ggatgaagga
     6061 tttcagggcg gctctaccat cgaccgcgac ccggatctgg ctattgtcgg aaccgaagat
     6121 cgggccttgt ttctgaccga acattatgct atggatgcgt tctcctgcaa agttcccaac
     6181 ggcaggtata tagccaagct ctactttgct gaaacctttg agggcattta cggcccgggc
     6241 gaacgggtgt tttcctttaa tgtgcaggga aaggaattca aggactttga tatttgggta
     6301 aaagccggcg gccccaaccg cgcgtatatt gaaacggtgc ctgtggaagt aaccaacggc
     6361 gagtttcgca ttacctttac atttcagcag gaaaatccgg ccatcaatgc tatcgaactc
     6421 atcccgcagc cggccgacag cgcatctggg gccgctgctg ctcctgcggc tcaagcccgt
     6481 ccagaccggc cgcgcggcgg attcggcggc ccgattgaac tggggccgga tgacaaagca
     6541 gtctttccgg acccgccggc cgattttcgg tcccagcgtg acaatatcgc ccgcggcagc
     6601 gaggaaatgg tcgaatacga ttccaaaaca gtcggcacgc gccgcaagat gctgatttat
     6661 accccgccgg gctactcgac cgaccgccgc tatccggtgc tttacctgct ccacggcatc
     6721 ggcggcgatg aaaccgaatg gcagcgtttc tgcagcccca atgtcatcct cgacaatctg
     6781 attgccgacg gcaaggcaga gccgatgatt attgtcatgc ccaatggccg ggcgcagaaa
     6841 aacgaccgtg ccgagggaaa tgtgtacaat accgcaccgg cctttgccaa ttttgaatac
     6901 gatttgctca atgatgtcat tcctgccgtc gaagcgcggt attcggtcta taaagaccgt
     6961 cagcaccgcg ccttggcagg tttaagcatg ggcggcggcc agtccctcaa tttcggcttg
     7021 gggcatctgg ataccttcgc ttggatcggc ggtttttcct cagcccccaa taccaaagcg
     7081 ccggcggaac tgattcccga ccctgctgcg gccaaagagc agctgaaact gctctatatc
     7141 agctgcggta acaaagacgg cctgattcgc atcagtcagg gccttcacaa ttatctgaaa
     7201 gaacacaata tcccgcatat ctggaatgtt gacagccacg gacacgatcc ggattcgtgg
     7261 ggcaagaatt tgtatcattt tgcacagcat ctcttcaaat aaccgcaagg agacaacaat
     7321 gaaaatcggg aaatggcaaa gcatcgtcct tctactgtcc atcgtatttt gtccggccgc
     7381 cagagctgcc gattccggcc ggccggcatt gaaagaagtg ttcaaggagt attttcttat
     7441 tggcggcgct ttgaaccgca atgtggtaac cggccgcgat cccaatgcgg cggcgattgc
     7501 cattgcgcat tttaataccg ccacgccgga aaacgatatg aagtgggcgc tgattcatcc
     7561 cgagccgggg cagtataact gggagccggc agaccgtttt gtggagttct gccagcaaca
     7621 ccagatgatt cccatcggtc attgtctcgt ctggcacagt caggtgccgc gctggacatt
     7681 tcaggatgat gccgggaatc ccttgacccg cgaagccctg ctggagcgga tgaaggagca
     7741 tatcaccgcc gttgtcaggc ggtacaaagg ccgcatcagg ggctgggatg tc
//
LOCUS       DAOTBO010000114         7697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8100_length_7697_cov_4.790238, whole genome shotgun sequence.
ACCESSION   DAOTBO010000114 DAOTBO010000000
VERSION     DAOTBO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8100_length_7697_cov_4.790238"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLE88_10950"
     CDS             <1..512
                     /locus_tag="PLE88_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM77025.1"
                     /translation="TYGTVVIDSIDWLLRRLEERVAGVNGDARNMDKTMNRSHGGYGN
                     GKQVLRNYVYQYLMPTLDAIVNRGISVVLLAHASRRSITTIDGIVMEKSAPEIHPDLM
                     NTMVEWSDFVGAARMDGDCRELVLNETAQLLAKNRYGITEVLLLDWNYFMNAMNNHKP
                     QLTGETING"
     gene            505..963
                     /locus_tag="PLE88_10955"
     CDS             505..963
                     /locus_tag="PLE88_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HOM77026.1"
                     /translation="MANLNNFNANEVEPNVGFEPVPADKYIAMITASEMKPTKNGDGS
                     YLELELTILEGPYKDRKIWDRLCLNHPNAQTVKIARGYLSSICRATGVMQPKDSCELH
                     NIPMCVTVKCKKRDDNDEITNEVRGYAKKESVAGKPQQAAPTDNTPPWKR"
     gene            960..1262
                     /locus_tag="PLE88_10960"
     CDS             960..1262
                     /locus_tag="PLE88_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77027.1"
                     /translation="MTMNKKAKGARLERKTIKMLQSAGYQCIRSAASLGPFDIIAMNH
                     LGFRCIQIKSNDWPRPDEREGLRAAAKSLPPNALIECWRWNDNAREPIIKLLDEFN"
     gene            1279..1524
                     /locus_tag="PLE88_10965"
     CDS             1279..1524
                     /locus_tag="PLE88_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77028.1"
                     /translation="MELVFSLILGACVGLIIGILIGLELAVKPDVNHQVDDLNSQAQK
                     SLNDFQETQCQIAHVSQKAETAHRDLKSKITELANQE"
     gene            1528..1668
                     /locus_tag="PLE88_10970"
     CDS             1528..1668
                     /locus_tag="PLE88_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77029.1"
                     /translation="MRFEKLFPTLLIILDICAALAYLPSGDWRRVVYWLAAAILTTCV
                     TY"
     gene            1671..3389
                     /locus_tag="PLE88_10975"
     CDS             1671..3389
                     /locus_tag="PLE88_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOM77030.1"
                     /translation="MQLRDYQQQSVDAVYDYLRNRDGNPCVVIPTAGGKTPVMSTICT
                     DAVNLWDGRVLVLAHVKELLEQTAGTLSQIAPDLDIGIYSAGLNRRDTEHSILVAGIQ
                     SVYKRACDLGAFDLVLIDEAHMIPPSGEGMYRTFLQEALVVNPNLRVIGLTATPFRMT
                     TGMICGPDNLLNEICFEVGVRELIVQGYLCPLKSKAGRQKADTSGLHLRGGEFIASEV
                     EDLMDQDALVSSACTEIIEQTTSRNSVLIFASGVSHARHIQTLLQQRVRQEVGLVTGD
                     TSAGERAELLARFKRQIVKADLFGNNKPPLKYLVNVNVLTTGFDAPNIDCVVLLRPTN
                     SPGLYYQMVGRGFRLHPSKEDCLVLDFGGNILRHGPVDSLQIKDSASGKGDAPAKECP
                     ECHSVVHAAYATCPECGYEFPPPERQQHDARASTAGILSGQTEDTEYPVYDTLYHHHM
                     KRNAGPDDPPTLRVEYNIGFNQYQSEWVCFEHTGYARNKAEGWWRARSNDPIPNTVQE
                     AIELAEAGALAPTEQITVRRVTGQKYDRIINYRLGPKPSLLDGSQERDDGNLPDYSWA
                     EDDIPF"
     gene            3401..3835
                     /locus_tag="PLE88_10980"
     CDS             3401..3835
                     /locus_tag="PLE88_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77031.1"
                     /translation="MTTYKGAFEHCSWCNGRGCNQCHFERKKYEDQFKIPQPLFSAEI
                     NDPEDMQLLKDVFGREAMEHAFGPDGGGMQEIEQAATLASFKQALRKHNKATPTMACP
                     QCGTEYEDFDGLGVLHCDSCGYCKHASITGGQCDYCGQAQGT"
     gene            3839..6076
                     /locus_tag="PLE88_10985"
     CDS             3839..6076
                     /locus_tag="PLE88_10985"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HOM77032.1"
                     /translation="MNHHIQQASIQYLQHGLSVLPAKRADKRPCIGSWKQYQKRLPTQ
                     TELDAWFSNPQDGICIITGQISGNLEIIDFDNGGELFDIWYQLIPADIRNRMVIEQTP
                     SGGWHVIYRCETEVSGNMKLAQRKDGNKIHTLIETRGEGGLFLCAPTAGYELMQGEFA
                     NLLNLTESEREILLEAAWSLNEYIPEPVQHSQHSDSSALRPGDDYNIRGNVIELLKSH
                     GWQFVNACGDNQRWRRPDKAKGWSATLRTTDNVFYVFSSNAAPFESNKAYSPFMVYTI
                     LEHGGDCSKAALELSKQGYGQPTSPAESDDVDLSGILSPPEDKPKTELFDPGQLPDEL
                     LRIPGFVSELMDFCLETAPYPNLGLAFCGALSMQSFLCGRKIRDPGNLRTNIYLLALA
                     GSSSGKDWPRQINSHIFMEIGKTDALGDKFASGEGIQDAMNLTASMLFQNDEIDGILI
                     SLNKSRDGRFESIMGTLLTMYTSSGSVYPMRRKAGKQSGGIIDQPHLTIFGTATPKYY
                     YESLSERMLTNGFFARMMVVDIGYRGTGQDAGLIDTMPKRILETAKWWDDFCPGQRRG
                     NLISFHPKPEVIPITNAARDVLDEFRYKADSEYAKSEAENDESAKSIWGRANENARKL
                     ALLYAASESYQSPEISQQGATWAVRFAEHQIRRMLYLVGQHAFENEFDKKCKQMVQIL
                     TTWQRQKGPHIWMPHWELGRRLKGWTERDFEMVRESLSARLEIEYEVGSTPKRGRVGF
                     RYRLC"
     gene            6424..7050
                     /locus_tag="PLE88_10990"
     CDS             6424..7050
                     /locus_tag="PLE88_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOM77033.1"
                     /translation="MSKNFTIELRNIDTIIPYDKNPRINDNAVDAVAASLKEFGFRQP
                     IVVDKDGVIVCGHTRYKAALQLDLKKIPVHVATDLTPEQIKAYRIADNKTADLAQWDY
                     EILPIELSELMDAGFDMNLLAFDEKELVKLLDTDVTQGNTDPDAVPEPPDEAITQPGD
                     VWILGNHRLMCGDSSSEADLDTLLDGKTIHLVNMDPPYNVCPMYPVAA"
BASE COUNT         2056 a         1822 c         2027 g         1792 t
ORIGIN      
        1 atacctatgg aaccgtggtt atcgactcga ttgactggtt gttgcggcgg ctggaagaac
       61 gcgtggccgg tgttaatggc gatgccagaa acatggacaa gaccatgaat cgttcgcatg
      121 gcggttacgg caacggaaaa caggtgcttc gcaactatgt ctatcagtat ctgatgccaa
      181 ccctggatgc cattgtcaat cgtggaatct cagtggttct tttggcgcat gcctcccggc
      241 gcagtattac caccatcgac ggcatcgtga tggaaaaatc agctcccgaa atccatccag
      301 acctgatgaa tacgatggtt gagtggtcgg actttgtcgg cgctgctcgc atggatggtg
      361 actgtcggga actggtgctg aatgaaaccg ctcagctgct ggccaaaaac cgttatggca
      421 tcacggaggt tctgctactg gactggaact actttatgaa cgcaatgaac aatcacaaac
      481 ctcaattaac aggagaaaca atcaatggct aatctgaaca atttcaatgc aaatgaagtc
      541 gaaccgaatg tagggtttga accggtgcct gcggataaat acatcgcgat gatcaccgcc
      601 tcggaaatga aaccgaccaa aaacggggac ggcagttatc tggaactgga actgaccatc
      661 ctggaaggcc cgtacaagga ccgtaaaatc tgggaccggt tgtgtctgaa tcatcccaat
      721 gcccagaccg tcaagatagc acggggatat ttatcctcga tttgccgggc gacgggcgtc
      781 atgcaaccca aggacagctg cgaactgcac aatattccga tgtgcgtcac agttaaatgc
      841 aaaaagcgtg acgacaacga cgagatcacc aacgaggttc ggggttacgc gaaaaaggaa
      901 agtgtcgctg ggaaacccca gcaggcagcg cctaccgata atacccctcc gtggaagcga
      961 tgaccatgaa caaaaaggcc aaaggggcac gtctggaacg aaagaccatt aagatgctgc
     1021 agtcggccgg ataccaatgt atccggtcgg cggcatccct gggaccgttc gacatcattg
     1081 ccatgaatca tctgggtttt cgatgtattc agattaaatc taatgactgg ccccgaccgg
     1141 acgaacgaga aggcttacgg gcggcggcga aaagcctgcc gcccaatgcc ctgattgaat
     1201 gctggcgatg gaacgataac gccagagaac ccattatcaa attactggat gaatttaact
     1261 gaatcgaaag gatcaaacat ggaacttgta ttttctctta ttttgggtgc ttgcgtggga
     1321 ttgattattg gaattttgat tggtttggaa ctggcggtaa agcccgatgt caatcatcaa
     1381 gtggatgatt tgaatagcca ggcacagaaa agtctgaatg attttcagga aacgcaatgc
     1441 caaatcgcac atgtcagcca aaaggcagaa accgctcatc gtgacctcaa atccaaaatt
     1501 acagaattgg caaatcagga atagacaatg cgatttgaaa agctgttccc aaccttgtta
     1561 atcattttgg atatttgtgc agcgctggca tatttgcctt caggcgattg gcgacgcgtt
     1621 gtgtactggc tggccgctgc aatcctgaca acctgtgtaa cctattgagc atgcaattac
     1681 gagattacca acaacaatct gtcgatgcgg tctatgatta tctccggaac cgggatggca
     1741 acccctgtgt tgtcatcccg accgccggag gtaaaacgcc agtgatgtcg accatctgta
     1801 cggatgcggt taatctgtgg gatggtcgag tgctggtact ggcccatgtc aaagaactgc
     1861 tggagcaaac ggcaggcacg ctcagtcaga ttgctccgga tttggatatt gggatttatt
     1921 cggcaggact gaatcgtcgc gataccgaac acagcattct cgttgcaggg attcagtcgg
     1981 tctacaaacg ggcgtgtgat ttgggtgcct tcgatttggt gttaattgac gaagcccaca
     2041 tgattccgcc ttcaggtgaa ggcatgtatc gtaccttcct gcaggaagca ctggtggtca
     2101 atccgaatct gcgggttatt ggtttaacgg ccacgccatt tcggatgacg acggggatga
     2161 tttgtgggcc ggataatctg cttaatgaaa tctgttttga agtcggtgtt cgggaactga
     2221 ttgttcaggg atacctgtgt cctttgaaaa gcaaggccgg acggcaaaag gcagatacat
     2281 ccggcctgca ccttcgcggc ggtgagttta ttgcctcaga ggtcgaagac ctgatggacc
     2341 aggatgcctt ggtctcatcg gcctgcacgg aaatcatcga gcaaaccacc agccgaaaca
     2401 gcgtgcttat ttttgcttcc ggcgtcagtc acgcccggca tattcagaca ctcctgcagc
     2461 aacgtgtccg gcaagaggtt ggactggtaa cgggtgatac atctgcaggt gaacgagcgg
     2521 aactgttggc ccgctttaaa cgccaaatcg tcaaggctga tttattcggc aataataaac
     2581 cgccattgaa atacctggtt aacgtcaatg tgctgacgac cggatttgat gcgccgaaca
     2641 tcgattgtgt ggttctgctg cgcccaacca attcacccgg tctttattat cagatggtgg
     2701 gtcgcggatt tcggctgcat ccatcgaaag aagattgttt ggtgctggac tttggcggca
     2761 acatcctgcg acatgggccg gtggatagtt tgcagattaa agacagcgct tccggcaaag
     2821 gtgatgcccc tgcaaaagaa tgccccgaat gccattcggt tgttcatgcc gcttatgcta
     2881 cctgtccgga gtgcggttat gaattcccac cgccggagcg tcaacaacat gatgctcgtg
     2941 ccagtactgc aggaattctg tcaggacaga ccgaagatac agaataccca gtctatgaca
     3001 cattgtatca ccaccacatg aaacgcaatg ccggtccgga tgatccccca acactacggg
     3061 ttgaatacaa tattggattt aatcagtatc aatccgaatg ggtgtgcttt gaacatactg
     3121 gatacgcacg aaataaagcc gagggctggt ggcgggcacg gagcaatgat ccgattccga
     3181 atacagtcca ggaagccatt gaactggccg aggcaggtgc acttgcccca accgagcaga
     3241 ttaccgttcg ccgtgttaca ggccaaaaat atgaccgcat tatcaattat cgacttgggc
     3301 ccaaaccgtc attgctcgat ggtagccagg agcgcgatga cggaaatcta ccagactatt
     3361 cgtgggccga agatgatatc cctttttaac ggagaattta atgacaacat ataaaggtgc
     3421 atttgagcat tgttcgtggt gcaacggacg tggctgtaac cagtgccact ttgaacgcaa
     3481 aaaatatgag gatcaattca agataccgca gccgctattt tccgcagaga tcaatgatcc
     3541 cgaggatatg caactgctaa aagatgtttt cgggcgtgag gcgatggaac atgccttcgg
     3601 accggatggt ggtggcatgc aggaaattga acaggctgcg acgctggcct ccttcaaaca
     3661 ggcactgcga aagcacaata aagccacgcc aacaatggcc tgcccccagt gcggcaccga
     3721 atatgaggac tttgatggat tgggtgtact gcattgtgac agttgcggct attgcaaaca
     3781 tgcttctatc acgggtgggc agtgtgatta ttgcggacaa gcacaaggga cgtagctggt
     3841 gaatcatcat atacaacaag catctataca atatttacaa cacggactat cggttctgcc
     3901 tgctaagcgt gcggacaaac gtccttgtat cggctcatgg aaacagtatc aaaagcgatt
     3961 gcccacccag acagaattgg acgcatggtt cagtaatccg caggatggaa tctgcattat
     4021 cacgggtcaa atttcgggga acctcgaaat tatcgatttt gataacggcg gcgagttatt
     4081 cgatatatgg tatcagctca ttcctgctga catccgaaat cgcatggtga ttgaacaaac
     4141 cccatccgga ggttggcatg tgatatatcg ctgtgaaacc gaggtcagcg gcaatatgaa
     4201 actggcccag cggaaagacg gcaataaaat tcacactctc atcgaaaccc gcggtgaagg
     4261 cgggctgttt ttatgtgcac cgacagccgg atacgaactc atgcagggag aatttgccaa
     4321 cctgttaaat ttaaccgaat ccgaacgcga aattctgctc gaagctgcat ggtcactgaa
     4381 tgagtatatt cccgagcccg ttcagcattc acaacattcg gatagttcgg ccttgcggcc
     4441 gggtgatgat tacaatatac gcggcaacgt tatcgaatta ctcaaatcac atggttggca
     4501 gtttgtcaat gcatgcggtg ataatcagcg ctggcgcagg ccggataagg ccaagggatg
     4561 gtcggcgaca ttacgcacca cggataatgt tttctatgta ttcagttcca atgcagcgcc
     4621 gtttgagtcc aacaaggcct attcaccgtt tatggtgtac accattctgg aacatggcgg
     4681 tgattgcagt aaagcagccc tggaattatc aaaacaagga tatggtcagc ccacgagtcc
     4741 agcggaatca gacgatgtcg acctctccgg tattctcagt cccccggaag ataaacccaa
     4801 aactgaatta ttcgatcccg gtcaattgcc agatgaatta ttacgcatcc cggggttcgt
     4861 ttcagaactg atggacttct gtcttgaaac cgctccctat ccgaatctgg gattggcatt
     4921 ttgcggcgca ttgagtatgc aatcctttct gtgtggtcgg aaaattcgtg acccgggaaa
     4981 cctgcgaacc aatatttatt tattggcact ggccggttca tccagcggaa aagactggcc
     5041 ccggcagatt aattcgcaca tcttcatgga aatcggcaag accgatgcac tgggggataa
     5101 gtttgcctcc ggtgagggta tccaggatgc gatgaatctg acagcatcga tgctgtttca
     5161 aaatgatgaa atcgacggga tactgatttc actgaataaa tcccgcgatg gccgttttga
     5221 aagtatcatg ggcacactgc tgaccatgta cacgtccagc ggctcggtct atcccatgcg
     5281 tcgaaaggca ggcaaacaat ccggtggtat cattgatcag ccgcatttga ctatcttcgg
     5341 gacggctaca cccaaatatt attatgaatc cctcagcgaa cggatgctga ccaacggatt
     5401 ctttgcacgt atgatggtcg ttgacattgg ataccgcggc accggtcagg atgccggtct
     5461 gattgatact atgcccaagc gaattctgga gaccgccaag tggtgggacg acttctgtcc
     5521 ggggcagaga cgcggtaatc tcatcagctt tcacccaaag ccggaagtga tacctatcac
     5581 aaatgccgct cgagatgtac tcgatgaatt tcgatataag gcggatagtg aatacgcaaa
     5641 atccgaggca gaaaatgacg aatctgccaa gtccatctgg ggaagggcca acgaaaatgc
     5701 ccgaaaactg gcgctgttgt acgcggctag cgaaagctac caatctccag agatctcgca
     5761 acagggcgca acgtgggcgg tcaggttcgc cgaacaccag atacgccgga tgctttacct
     5821 ggtaggtcag cacgccttcg aaaacgaatt tgataagaaa tgtaagcaga tggtgcagat
     5881 tctcaccacc tggcaaagac agaaaggacc ccatatatgg atgcctcact gggagctagg
     5941 caggcggcta aaggggtgga ccgaacgaga ctttgaaatg gtgagggaat cgttgtctgc
     6001 acgtttggaa attgaatatg aagtgggtag cacacccaaa cggggacggg ttggattccg
     6061 gtatcgactg tgctaattac cgcattttgg gcatgtggta attaacgtta tgcggtaatt
     6121 aaacccccta aaccagctaa ttaccacatg gattgccaat taccacgtaa ttaccgcata
     6181 gccaaatgtg gtaattaaat ttcgtaaatc cttataaata aagaatataa tatatatacc
     6241 acataaacac atatatatac ctcacgatct gtaagcgcgc gtacacgcgt aggttttgcg
     6301 gtaattaacg tttaggtact tccccgcgat tcaagacgtg actgcacggg aacgcgtcgc
     6361 attgttatac acagtttgtt tgcgctgtcc ggttttttaa atcacacttt tttggagatt
     6421 tctatgagca agaactttac catcgaactt cgtaacatcg acaccatcat cccctacgac
     6481 aaaaatcctc gcatcaatga caacgctgtt gacgcggtgg cggcatccct gaaggagttt
     6541 ggctttcgcc aacccattgt ggtcgacaaa gatggcgtca ttgtctgcgg gcatacccgc
     6601 tacaaggcgg cgctgcagtt agacctgaag aaaatccccg ttcatgtggc gacggattta
     6661 acaccggagc agataaaggc ctatcgcatc gcggataaca agactgcgga tttggcgcag
     6721 tgggattacg agattctgcc gattgaatta tccgagttga tggatgccgg tttcgatatg
     6781 aacctgctgg catttgatga aaaagagctg gtcaagctgc tggatacgga tgtcactcag
     6841 ggcaataccg acccggatgc ggttcccgaa ccaccggatg aggcgattac ccagcccggc
     6901 gacgtctgga tactcggtaa ccatcgcctg atgtgcggtg actcatccag cgaagctgac
     6961 ctggacacat tgctggacgg caaaacaatt catttggtta atatggaccc accttacaac
     7021 gtatgcccga tgtatcctgt tgcagcataa ggacttacag taattatgct taacagggat
     7081 gccttgctgg caatgtttaa cttgaaccgt gactgtttgg ccagtcgttg tacctatgga
     7141 gtgagttggc atgaaatgcg agtatgccgg caaaacaatt ctccagagaa cgtgggacac
     7201 aggcgaggct tcatagccgt tgcagtcctg ccaggtagag ttttcgcatg actacagaaa
     7261 gaaaccaacc aacaccttcg ggaaagcggg cccccattcc tgctaatacg gaagccttcc
     7321 gagattacct ccctcggaat agggaatggc ccaaacggat gagcgctctc gttgtaggcg
     7381 aacatcccgg agaatccccc tcgggattcc aaggtcggca aagcggccaa ctatttgccg
     7441 gtgtttgggg gagcagcaga atgctgtcag gtgacgaaag gttggagtct aaccgaaaac
     7501 gtttgagtgt cagcaaggga accggggaag cccggccgag tatggtagtc gcatggatag
     7561 gctgtcatat gacagttgaa tccctaccga tgaggcggtc gggtggcgga gcacccgtag
     7621 tagtccgagg acgggaaagc cgtccacatg gcgaaggggt gcaggatgtt tcgttttgga
     7681 tgacggaaag gttcaat
//
LOCUS       DAOTBO010000115         7494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8371_length_7494_cov_5.123807, whole genome shotgun sequence.
ACCESSION   DAOTBO010000115 DAOTBO010000000
VERSION     DAOTBO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8371_length_7494_cov_5.123807"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /gene="wecB"
                     /locus_tag="PLE88_10995"
     CDS             complement(<1..985)
                     /gene="wecB"
                     /locus_tag="PLE88_10995"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOM77034.1"
                     /translation="MIKIICVCGARPNFMKIAPIMRAFNQSGKFQTLLVHTGQHYDAK
                     MSQLFFDELAIPKPDVNLEVGSGSHAVQTAEIMKRFEPVVLDFKPDYVLVVGDVNSTI
                     ACGLVAVKLGVKLIHVEAGLRSFDRSMPEEINRILTDSISDLLFVTEQSGVDNLKREG
                     VNSDKVYLVGNVMIDTLLANRNKAEQSPILEQLGLEKRKYAVITLHRPSNVDCLEGLE
                     KIIAAFEVIQKDLKLVFPIHPRTKKNIEGSALKGRVEAMQNLILLEPAGYLDFLKLTA
                     NAALVLTDSGGIQEETTILGIPCLTLRENTERPITITEGTNQLVKLTTQDIL"
     gene            complement(982..2211)
                     /locus_tag="PLE88_11000"
     CDS             complement(982..2211)
                     /locus_tag="PLE88_11000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77035.1"
                     /translation="MRILVVSNLYPYQSNKVYGIFTARQFAMAARLGADVTVLFTQVW
                     IPSILQRFGPFKNYDCSHTPIDYPGIRVIRVPCVRWTRGIGGLRWDGLMFYASGRKVA
                     EQLHREKPFDIIYGKGVLPCGDGAVRLKKLLGIPAAAEGIGGDVNIAPDYSPAMYRHF
                     LWVTRQLDGAVADGKGVADRLSRVMQKEVPTIHGLVDMEIFKPISDKRNKLELRRQLN
                     IPEQSPAILFVGNLKREKGLYELLEAMVRIRGRIVGATLTICGRGSEQEGLKAKIAEL
                     GLGDSVRLAGAVDPADMHRWMQAADIFVLPSYTEGMPNVVMEAMACGLPTISTTVGGL
                     PDGVGDSEGAILVPPQQVEPLTEAILRVCGDKLLQDKMSAAARQTAEQKFGLENNVRK
                     TLNHLQSIIVKRRTIAL"
     gene            complement(2208..3629)
                     /locus_tag="PLE88_11005"
     CDS             complement(2208..3629)
                     /locus_tag="PLE88_11005"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOM77036.1"
                     /translation="MKIMLEQDYYLEGYGQPGLTETVSEDYLRLVRCFWVFGTAAVIA
                     AAVFVFTGNLALAGGICGFVGVVLSVFHIPFGLFILFSLLSIENAVQLHPYFTVSKAM
                     GVVVLFSFLLHMFRAKLIFDRNLNLMGAFVLWGVCTSIWSKSPYWALLNCLSVALSIG
                     LIVIMLNTVQDKKSLFLVLSGFAAGAFVASFMVVTGRVAERSFSSPDIERATLSEGAN
                     TLVLALALGLGFLVVFYMFFMPGKLKKIAAIGLLPLIFLAMVKTQSRMPTFAAISIPV
                     FALILGSKSQYRFRYFCIAVILGLIAYVSIKLVIQSDLMTEAARKRLTEQGFGESGRL
                     QFWKWGLEAFVYRPLHGYGFNNFHLIPNNVGQKAAHNTFIAVMVDTGLIGLSLAIAIV
                     ITLYKQVMRLSDVGLKWLGLAMLMYPMMTGITVTNYIKKDFWYALGIVVVVIHIGKLQ
                     EERAREQLEYPLYDTELFQKANG"
     gene            complement(3646..3801)
                     /locus_tag="PLE88_11010"
                     /pseudo
     CDS             complement(3646..3801)
                     /locus_tag="PLE88_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
     gene            complement(4152..5408)
                     /locus_tag="PLE88_11015"
     CDS             complement(4152..5408)
                     /locus_tag="PLE88_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77037.1"
                     /translation="MRSRKYAQYLEDFGWECSVLTRCFGRDRKVHFPGTVTITSVYAP
                     DLSQWLGQLVRIVQSLRRWLRKKCSTVGSTNNPSAAAALSSDGMGMATQIQRMFLLPD
                     TQFLWILAAIPFALWKQRDCRVIYTSAWPVSCHVLGLLVHKLTGKPWVADYRDEWTLN
                     TQWLPPTRFHRWLGEKLDAACVRNARFVVNTTEIRTQNFIHKFGGNPNKYVTIHNGYD
                     QQDIAPYLALTPPAEVLTLTSIGSLYGGRDPRPLLNTLADCVHSGLIDKQQLLVRLIG
                     VQSESLQSQIDQLGISECVHVIPRIPQLQAFEHLAQSHIALLIGSEMERYAMTTKLYE
                     YVGMGKKIFALVPPGPVQEFVVRCGGICAMYNDTTQIKQALMVIFDQFRQGLLSQNSP
                     PNQTYERRNLAGQFAALLDACLTAAE"
     gene            complement(5489..6952)
                     /locus_tag="PLE88_11020"
     CDS             complement(5489..6952)
                     /locus_tag="PLE88_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOM77038.1"
                     /translation="MMFQGLTQATQLILGMVLVRLISKEMLGSYRQVALSYALIAGIF
                     SLQLESSLYYFMPKLGPQRRRELLTQTLLTTGLLSLVISLAMFLTAGLFAAQFKNPQL
                     ATWIRIFALFPLFERIIQLQPAFLISLDKALYSGLYSMLSSLLMIGAVMSVFAAGYGV
                     TEAVWAKVFIGALSAAAGIVLMAAFSRESQWRFSKELWLEQVNYCWPLMATTIVGIIN
                     LKMDGVLISSYFSKEVYAVYSTGAMELPLLALFTSSLASAIMPNMVAEAGKGNLSGAL
                     NLWHESTRKSSLLIFPTFVFFLVCGYDFIVLLYTPDYSNAAWPFLIYLARLPVRVAIY
                     ASIFRAIGHTKPLAVAALLSFVSNMIVSVILLFAGRHGFLSYIGPAIGTFFGTAVSVT
                     FLLLLLCRKMNIRFAQVMRWKELGRIFGLALLCGGLLWLIPIPFESLLLKLIGRFILF
                     MLFYICALIFTKSLKPDEWELLLLPLALVRKIIRKES"
     gene            complement(6989..>7494)
                     /locus_tag="PLE88_11025"
     CDS             complement(6989..>7494)
                     /locus_tag="PLE88_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOM77039.1"
                     /translation="YIALILEIIRYFLDHTDHNVILIPHEASLNRPNDPELCMLLKEL
                     ARNPDRVAMLTGKESALEVKSVIGQSDFLVASRYHSLVAALSMRTPVAVIGWSHKYDE
                     LMQVVGLSDYVIDPVRNLENKDVIEMVKKAYQKRQEIAFQLKEKLPLIENNVHVVFEK
                     IMAILDR"
BASE COUNT         2083 a         2105 c         1615 g         1691 t
ORIGIN      
        1 gcaggatatc ctgtgtcgtc agtttgacca attgattggt accttcggtg atcgtaatag
       61 gccgttcggt gttttcccgc agtgtcagac agggtatgcc caaaattgtc gtttcttcct
      121 ggatgccgcc ggaatccgtc aatactagtg ccgcattggc agtcagtttg agaaaatcca
      181 gataccccgc cggctccagc agaatcaaat tctgcatcgc ttccacacgt ccttttaagg
      241 ccgaaccttc gatattcttt ttcgtgcggg ggtgtatagg aaagaccagc tttaaatctt
      301 tctgaatcac ctcaaaagcc gcgatgatct tttccagtcc ttccagacaa tcgacattac
      361 tcgggcggtg cagagtaatc accgcatatt tcctcttttc caaccccaat tgctccaaaa
      421 tcggcgattg ttcggccttg ttgcgatttg ccaaaagcgt atcgatcatc acattgccga
      481 cgagatatac tttatccgaa tttacacctt cacgcttgag attgtcaacg ccggattgct
      541 cggttacaaa caaaaggtcg ctgatactgt cggtcaaaat acgattgatt tcctccggca
      601 tcgaacggtc aaagctgcga agccccgcct cgacgtgaat cagcttaacg ccaagtttca
      661 ccgccaccag cccgcaggca atggtgctgt tgacatcgcc gaccacaagc acatagtcgg
      721 gcttaaaatc caaaaccacc ggttcaaacc gcttcataat ctcggccgtc tgcaccgcgt
      781 gcgaaccgct gccgacttcc aaattgacat ccggcttagg gatagccaat tcatcaaaaa
      841 ataactggct catcttggca tcgtagtgct ggccggtgtg caccagtaat gtctgaaact
      901 tgccgctttg attgaaggca cgcataatcg gggcaatttt cataaaattc ggccgcgccc
      961 cgcaaacaca gataatttta atcataatgc tatagttctc cttttaacaa taatcgattg
     1021 cagatggttc agcgttttgc gtacattgtt ttcaagccca aacttttgct cggccgtttg
     1081 ccgtgcagca gcagacatct tgtcctgcaa tagcttatcc ccacagaccc gtaatatcgc
     1141 ctcagttagc ggctcaacct gttgcggcgg cacgagaatt gccccctcac tatcgccgac
     1201 gccatccggc agcccgccga cggttgtgct gattgtcggc agaccacacg ccatcgcctc
     1261 cattaccaca ttgggcatcc cttctgtgta cgacggcagc acaaagatat ccgctgcctg
     1321 catccaccga tgcatatctg ccggatccac cgccccagcc agacgcacac tatcgcccag
     1381 tcctaactcg gcaatcttgg ccttgagccc ctcttgttcg ctgccccgcc cgcagatcgt
     1441 cagcgtcgcc ccgacaatcc gcccgcgaat ccgaaccatt gcttccagca gttcatatag
     1501 ccctttttca cgcttaagat tgccgacaaa tagaatcgca ggcgattgct cgggaatatt
     1561 taattggcgt cggagctcta atttattgcg tttgtcgctg atgggtttaa atatctccat
     1621 atccaccagc ccatgaatag taggaacctc tttctgcatc actcgggaca atcggtcggc
     1681 tacaccctta ccgtctgcca ccgcaccatc caactgccgt gtgacccaca agaaatgccg
     1741 atacatcgcc gggctgtagt ccggggcgat attgacatca ccgccaatac cctcggctgc
     1801 cgccggaata cccagcagct ttttgagccg caccgcccca tctccgcagg gcaacacacc
     1861 tttgccgtaa ataatatcga acggcttttc acgatgcagt tgctcggcaa ccttacggcc
     1921 agatgcatag aacatcagcc catcccagcg caatccgcca atcccccgcg tccaccgcac
     1981 gcatggcaca cgaatcaccc gaatccccgg atagtcgata ggggtatggc tgcaatcgta
     2041 atttttaaat ggtccaaacc gctgcaggat cgacggtatc caaacttgcg taaacagcac
     2101 cgtcacatcc gcccctaacc gtgcagccat ggcaaactga cgcgccgtaa aaataccata
     2161 cactttattg ctctggtagg gatataaatt cgaaacaaca aggattctca tccattggcc
     2221 ttctgaaata gctccgtatc atacaaagga tattcaagct gttctctggc acgttcctcc
     2281 tgcagttttc caatatggat cacaacaacc acaatgccca acgcatacca aaagtctttt
     2341 ttgatatagt tggtcactgt gattcctgtc atcatcggat acatcagcat cgccagcccc
     2401 agccatttta gccctacatc ggatagccgc atgacttgtt tgtaaagtgt tataacaatc
     2461 gcaatcgcca gcgacaagcc aatcaaccct gtatccacca tgaccgcaat gaaggtattg
     2521 tgagcagctt tttgtcctac gttgttcggg atcaagtgaa agttgttaaa tccatatcca
     2581 tgcagcggac gatagacaaa ggcctcaaga ccccatttcc aaaattgcaa tcgtccgctt
     2641 tctccaaatc cctgttcggt caatcgtttt ctggccgcct cggtcattaa gtcgctttga
     2701 ataaccaatt tgatagaaac atatgcaatt aaacctaaaa tcacagcgat acaaaaatac
     2761 cgaaagcgat attgactctt ggaccccaaa atcagcgcaa agaccggtat cgatatcgct
     2821 gcaaatgtcg gcatacggct ttgggtctta accattgcca gaaaaatcaa tggaagcaaa
     2881 ccgatagccg caatcttttt aagtttaccc ggcataaaaa acatatagaa caccaccaga
     2941 aagccaagcc ccagcgccaa tgccaacacc aaggtattgg caccctcgct taaggttgcc
     3001 cgttcaatat ctggcgaact aaaacttcgt tcggccaccc gtccagttac aaccataaat
     3061 gaagccacaa atgccccggc agcaaagcca ctcagcacaa gaaacaggct tttcttgtct
     3121 tgtaccgtgt tgagcataat cacaataagc ccaatgctca atgcaacgga taaacaattc
     3181 aataatgccc aatagggcga cttggaccag attgaagtac atacacccca gagcacaaac
     3241 gctcccatca aattcaggtt gcgatcaaaa atgagcttgg cacgaaacat atgcagcaga
     3301 aaactaaata ataccacaac ccccatcgcc ttgctgaccg taaagtaggg atgcagttgg
     3361 actgcatttt caatcgacaa cagggaaaac aagataaaca gtccaaacgg tatatgaaac
     3421 accgacagca ccacacccac aaaaccgcaa ataccgcctg ccagagcaag attaccggta
     3481 aagacgaata ctgccgcagc gataaccgca gcggttccaa acacccaaaa acaccgcacc
     3541 aaccgcaaat aatcctcgct gaccgtttcc gtcaatcccg gctgaccgta accttccaga
     3601 taatagtcct gctcaagcat aattttcatc catttgctgc tgatttcgat atctgataac
     3661 acgtgccggg ttgccgccga caatggcata ctccggaacg tctttggtca ccaccgcccc
     3721 agctccaata attgcaccct taccaatttt gcgccccggc agtataattg cccgggtacc
     3781 aatccataca tcatcgccga tataaacagg tcttagcggc tctccgccct gctgacacat
     3841 cggtatatca gttctatcaa atttgtgact ggcagataaa accacaacat cggggccaat
     3901 cattacatca tttccaataa cagccgcacc tatccagcat ctaacaccta agccagaatt
     3961 gtcaccgatt tcaatatgcc gccctgaacc aaaatctgcc ccgtgttcga tattgacatt
     4021 cttgccgagc aaacaaatgt cggcagatcc aataccgaat ccgctttgct ccgcaggcat
     4081 agggcttata cgacaccggc aaatgccgcg caaaaccata atacagcaaa taccagaaaa
     4141 cccttgtcat attattcagc agcagttaaa cacgcatcca gcagcgctgc gaactgaccc
     4201 gcaagatttc gccgttcata ggtctggttg ggtggactgt tttgacttag caagccctga
     4261 cggaactggt cgaagattac cattaaagcc tgtttgatct gagtcgtatc gttgtacatc
     4321 gcacaaatcc cgccgcagcg gacgacaaac tcctgcaccg gaccgggcgg cactaacgca
     4381 aagattttct tgcccatccc gacatattca tacagcttgg tcgtcatcgc atagcgttcc
     4441 atctccgagc cgatcagcaa tgcaatgtgg ctttgtgcca gatgctcaaa agcctgcagc
     4501 tgggggattc gcgggatcac atggacgcac tcacttatgc ctaattgatc gatttgcgat
     4561 tgcagcgact cgctctggac gccgatgaga cggacaagca attgctgctt atcgatcagg
     4621 ccgctgtgaa cgcaatcagc caaggtgttt aacaggggcc gcgggtcccg accgccgtat
     4681 aaactgccaa tgctggtcag ggtcaatacc tctgccggcg gcgttaatgc aagataaggc
     4741 gcaatatcct gctggtcata gccattgtgg atcgttacgt atttattagg attaccgccg
     4801 aatttgtgga taaagttctg ggtgcggatt tccgtggtat tgaccacaaa cctggcattt
     4861 cgcacgcagg ccgcgtcgag cttttctccc agccagcgat gaaaccgcgt tggcggaagc
     4921 cactgtgtgt tcagtgtcca ttcatctcgg taatcggcta cccagggctt gccggttagt
     4981 ttatgcacca acagccccag aacatggcag ctaactggcc atgccgaggt gtaaatgact
     5041 cgacaatcgc gctgtttcca caatgcaaaa ggaattgccg ccaaaatcca caaaaactgt
     5101 gtatccggca gcaaaaacat ccgctgaatc tgggtcgcca tccccatacc atccgaggat
     5161 aaggccgctg cagcagaggg gttgttagtg gagccaacgg ttgaacattt ctttctaagc
     5221 catcgcctca gcgattgcac aatcctaacc agttgaccca gccattgaga aagatccgga
     5281 gcatagacag aggtaatcgt caccgtgccg ggaaaatgga cctttctgtc gcggccaaaa
     5341 caccgggtca ggacagagca ttcccaacca aaatcctcca agtattgagc atattttctg
     5401 gaacgcaaag agccaacgcc catatcgggc ggaaactgat agcagatcat caggacctat
     5461 tttttaggag attcggaagg ggtcatgtct aagattcttt tctgataatt tttcggacca
     5521 gcgccagcgg gagcagcagc agttcccatt catccggttt taggcttttt gtgaatatta
     5581 aggcacagat gtagaatagc ataaacagga taaaccggcc gatcagcttt agcagcagag
     5641 actcaaacgg gatcgggatc agccacaaaa gcccgccgca cagcagtgcc agcccaaaga
     5701 tacgccccaa ctccttccat cgcatcacct gagcaaaccg aatgttcatt ttgcggcaca
     5761 gaagcagcag caaaaaagtg accgacaccg ccgtgccaaa gaatgttccg atagcaggac
     5821 caatgtagga caaaaacccg tgccgacctg caaacagaag tataacgctg acaatcatgt
     5881 tggaaacaaa ggacagcaag gccgccaccg caaggggttt ggtgtgaccg atggcgcgga
     5941 agatcgacgc gtaaatagcc acccgaactg gcagacgcgc tagatagatc aaaaacggcc
     6001 atgccgcatt ggaatagtcg ggggtataga gcaagacaat aaagtcatac ccgcacacca
     6061 gaaagaacac aaaggtcgga aagatcagca gactgctttt gcgggtggac tcatgccaaa
     6121 gattaagcgc acccgataaa ttccccttac cggcctccgc caccatattg ggcataatcg
     6181 ctgaggctaa gctggaggta aacagggcga gcagcggcag ttccatcgcc cccgtcgaat
     6241 acaccgcata aacttctttg gagaaatagg aggatatcaa aacgccgtcc attttgagat
     6301 taataatccc aacaatcgtt gtcgccatca ggggccagca gtaattgacc tgttccagcc
     6361 acaattcctt actgaatcgc cactggcttt ctcgtgaaaa agccgccatc agtacaatac
     6421 ccgctgcggc agacaacgct ccgataaaca ccttggccca aacggcctct gtaaccccgt
     6481 atcctgcggc aaagacgctc atcaccgctc cgatcatcag cagcgaacta agcatactgt
     6541 aaagaccgga atacagggcc ttgtccagac tgatcaaaaa ggccggctga agctggataa
     6601 tccgctcaaa gagcggaaat aaggcaaata tcctgatcca ggtcgcaagc tggggatttt
     6661 taaactgggc ggcaaacaga cccgccgtca gaaacatcgc cagactgatc accagtgaaa
     6721 gcagtcctgt tgtcagcagg gtctgggtca atagctctcg ccggcgctgg ggccccagct
     6781 tgggcataaa atagtataga ctgctttcaa gctgcagtga aaagataccc gcgatcaggg
     6841 cgtaagacag ggcaacttgc cggtaactgc cgagcatctc cttgctgatc aggcgcacca
     6901 gcaccatccc aaggatcaac tgagtcgcct gcgttaaccc ctgaaacatc atgattaaaa
     6961 tgctacgggt gcgaatacgc ataaaaactc aacgatctaa aatagccata attttttcaa
     7021 aaacaacgtg aacattattt tctattaaag gcaacttttc cttaagctgg aaagcaatct
     7081 cttgtcgctt ctgatacgcc tttttgacca tttctatgac atctttattt tcaagatttc
     7141 taacaggatc aataacataa tcggataagc cgacaacctg catcaactcg tcatacttgt
     7201 gggaccagcc aatcacagca accggtgtcc gcatcgaaag tgccgccacc aggctgtgat
     7261 aacggcttgc cactaaaaaa tcagactggc caataacaga cttaacctca agggctgatt
     7321 cttttccagt caacattgca acacgatcag ggttcctcgc caactcttta agcaacatac
     7381 acaactccgg gtcattgggc ctattgagtg aagcttcatg gggtataagt ataacattat
     7441 gatctgtatg atctaaaaaa tatcgaatga tttcgagaat aagagcaata tact
//
LOCUS       DAOTBO010000116         7470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8402_length_7470_cov_4.149697, whole genome shotgun sequence.
ACCESSION   DAOTBO010000116 DAOTBO010000000
VERSION     DAOTBO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8402_length_7470_cov_4.149697"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLE88_11030"
     CDS             complement(<1..416)
                     /locus_tag="PLE88_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOM77040.1"
                     /translation="MIQNVNATRMELLALRKRVALALRGHRLLSEKRDEICRQLVQIA
                     RDLRDLRSRVERDLLETSRRFMMARAVMEPEHIRAALDVPSKTFKLAVSFASIMSVQV
                     PRLAKEIAGDILCYGFSSTSGELDAALRALERAFDGL"
     gene            complement(413..1810)
                     /locus_tag="PLE88_11035"
     CDS             complement(413..1810)
                     /locus_tag="PLE88_11035"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOM77041.1"
                     /translation="MLKEYKTVSQISGPLMMVEGVDNAKYGHIVDIELADGSMRHGQI
                     LQVEGDKVLVQVFEGTESINVSGTTVRFLGKPMEMAVSRDILGRVFSGTGVPIDGGPR
                     ILPKMKLNINGNPINPYARDYPNEFIQTGISTIDGLNPLVRGQKLPIFSGAGLPHDEI
                     TAQLARQATVRGKGEAFAVVFAAMGITFEAAQFFINDLTNTGAIERAVLFINLANDPA
                     IERIAAPRVALTAAEYLAFEEDMHVLVILNDMTNYCEALREISAARKEVPGRRGFPGY
                     LYTDLATIYERAGRIKGKKGSITMVPVLTMPEDDKTHPVPDLTGYITEGQIIMSRSLH
                     RKAVAPPVDVLPSLSRLKDKGIGPGKTREDHAALYNQLYAAYARGKEAQELATILGEA
                     ALSAEDQKYMKFANAFEARYISQGYYENRDIMQTLDLGWELLSMFDDVELKRIDKKLI
                     DKYMPRFRKQQGAAA"
     gene            complement(1839..>2903)
                     /locus_tag="PLE88_11040"
     CDS             complement(1839..>2903)
                     /locus_tag="PLE88_11040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOM77042.1"
                     /translation="TGKTVVQHQLAKWVDADVIVYVGCGERGNEMTDVLMEFPELTDP
                     HSGQPLMKRVVLIANTSDMPVAAREASVYTGITIAEYYRDMGYSVALMADSTSRWAEA
                     MREISTRLEEMPAEEGYPAYLGARIASFYERAGRVVCAGSPQREGALSVIGAVSPPGG
                     DLSDTVVQATLHVVKVFWSLQGRLAQQRHFPAIDWLTSYSFYKQYLPESFKDKQMGRE
                     FAEMMQQAMTLLEQEAQLVEIVRLVGAESISARDRMTLETAKSIREDFLHQNAFHEVD
                     TYTPIEKQYEMIKLILHFHQAGLAAIDSGADTAGLFKLPVREAIARAKYLDNDKIEQV
                     AAIRDVIDRQVKELLAAAAE"
     assembly_gap    2904..3003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3004..3772)
                     /locus_tag="PLE88_11045"
     CDS             complement(<3004..3772)
                     /locus_tag="PLE88_11045"
                     /inference="COORDINATES: protein motif:HMM:NF028196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77043.1"
                     /translation="MTNVHNGRIIKVAGPVVVAEGMLGARMYDVVRVGRENLIGEIVG
                     LHGDRASIQVYEDTIGIGPGEEVVNTGAPLSVELGPGLISSIYDGIQRPLDQLAAAQG
                     SFIKRGSAIPGLQRQTRWHFTPTVSGGTDVQSGDIIGQVQESTLVLHKIMVPPGVSGR
                     IDGLSEGDYTVDQPIATVTAADGTKTELKLMQKWPVRQPRAIRQRLNPNHVLLTGQRV
                     IDAFFPIARGGTACVPGPFGTGKTVVQHQLAKWVDADV"
     gene            complement(3812..4138)
                     /locus_tag="PLE88_11050"
     CDS             complement(3812..4138)
                     /locus_tag="PLE88_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOM77044.1"
                     /translation="MMMEGKAAVLGSADFVMPFAAMGLDTFAVEADAAAVQAQAARIL
                     RDKYALVVVAENVAQAAQGVFEAVQKAAMPCVIVVPFTTEPTGIATESLGRMLKMATG
                     INILKN"
     gene            complement(4158..5195)
                     /locus_tag="PLE88_11055"
     CDS             complement(4158..5195)
                     /locus_tag="PLE88_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOM77045.1"
                     /translation="MTIGSDISFYRYPPIGQEDWRYAYPTARVRALELAMLPRSVFAD
                     MANAASFADAAELLAGTEYAVDAQTDAARLEQMLLERRSEVRRLFSQLILDEGILTFL
                     RAREDFANMRLAVRRVVTERPLGLDYSNDGNVPAEEFEDIFAQENYERLPDYLQEAVE
                     AAILGYYENRDIRRIDYEIDRMYAAWRIRQAEALKDVFCLSLMRIRNDLDNLRMMLRL
                     KMAGREEDTRLFLPPGFVETDRFVQGLAAGWEAVAAGFYATPYYELLETSIPYLRSEQ
                     SFLKLEKECEDYVKGFLKTTRSIPAGPQPVIAYFLMKEAEIRTVRMVLTGKKNGLAAK
                     LILDRLGEWMP"
     gene            complement(5217..5810)
                     /locus_tag="PLE88_11060"
     CDS             complement(5217..5810)
                     /locus_tag="PLE88_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOM77046.1"
                     /translation="MNAEQVVEKILSQARAEAEAILGQARQKAAQADAQLEAELAEFD
                     RKTEQLAEAAGQDRWQRMLAAARMDNARRLLAARTAILDEVFAKARQALCQMPDAPYQ
                     ALMADLMTKAVETGDEEVVIGRGETRIHEPFLAMVNSRLGPDRGRLRLCPQRGDFEGG
                     FILRRQRVQINASAEVLVDRLRDAMEMELAGMLFGTD"
     gene            complement(5851..6339)
                     /locus_tag="PLE88_11065"
     CDS             complement(5851..6339)
                     /locus_tag="PLE88_11065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOM77047.1"
                     /translation="MLEMMGLTFALTGAALAVFLAGIGSAVGLAAASKSATGVLSEKP
                     ERYGTLMLLVVLPSTQGIYGFVVGLFVMIKLNMFGGSVNPVAWHQGLEILAACLPVAF
                     GGLFSAIHQGRTGAAGILMTAKRPEMAFKAGVVYAAMIELYAILGFLISLLILLMGIK
                     VG"
     gene            complement(6379..>7470)
                     /locus_tag="PLE88_11070"
     CDS             complement(6379..>7470)
                     /locus_tag="PLE88_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77048.1"
                     /translation="KKDYPKLEALVGQFDGCDIAPIEPAEGEEIPIEIDNNRAVRPFE
                     VITRLYGMPLPSSVDPTVFLAPFFAVFFGLCLADAGYGLIMMGLLAWILKKTRGDKGV
                     FWMLLVCGFTTFLAGAITGSWFGDAVTALLPAQSAVGSALNWMRCKIMLFDPMTQPMT
                     FFALSLGVGYLQIQFGLFIAFFANLLKKDIAAAVFDQLTWIVMLNCLVVLGLSKGELL
                     PAGLAKPVGLAALLPAVLILLFSGRGMGWGGRVGFGVFQLFSTVFYMGDILSYVRLMA
                     LGMVGSGFGLAINVLVKLVADVPYVGWLLGAVLFVGGHLFNLAMSMLGAFVHSLRLQF
                     VEFFPKFFVGGGQEFTPLRRQYRYIDLKE"
BASE COUNT         1478 a         2358 c         2077 g         1457 t
ORIGIN      
        1 agcccgtcga aggcccgctc cagcgcccgc agcgcggcat ccagttcgcc gctggtggat
       61 gaaaagccgt agcacaggat gtcgccggcg atttccttgg ccagccgcgg cacctgcacg
      121 ctcataatcg aagcaaaact gaccgccagc ttgaaggtct tggacggcac atccagcgcc
      181 gcccggatat gctccggctc catcaccgcc cgcgccatca taaaccgccg gctggtttcc
      241 agcaggtcgc gctccacgcg gctgcgcaga tcccggaggt cgcgggcaat ctgcaccagc
      301 tggcggcaga tttcgtcgcg tttttcgctg agcaggcgat gcccgcgcag cgccagcgcc
      361 acccgctttc gcagcgccag caattccatt cgtgtggcat tgacattctg aatcatgcgg
      421 cggcaccctg ctgcttgcgg aaccggggca tatacttgtc aatcagcttc ttgtcgatgc
      481 gcttgagctc tacatcgtca aacatgctca gcagctccca gcccaaatcg agcgtctgca
      541 ttatatcgcg gttttcataa tagccctgcg agatataccg ggcctcaaag gcattggcaa
      601 acttcatata cttctggtct tcggccgaca gggccgcttc gcccagaatc gtcgccagct
      661 cctgcgcctc tttgccgcgg gcataggcgg catagagctg gttgtacagt gcggcgtggt
      721 cttcgcgggt tttgccgggg ccgatgccct tgtctttcag acgcgacaga ctcggcagca
      781 catccaccgg cggcgccacg gccttgcggt gcagcgagcg gctcataata atctggcctt
      841 cggtgatgta gccggtcagg tcgggcaccg gatgggtctt gtcgtcttcg ggcatggtca
      901 gcaccggcac catggtaatc gagcccttct tgcccttgat gcggccggcc cgctcgtaaa
      961 tcgtggccaa gtccgtatag aggtagcccg gaaagccccg ccggccgggc acttccttgc
     1021 gggcagccga aatttcccgc agcgcctcgc agtaattggt catgtcgttg agaatcacca
     1081 gcacatgcat atcttcctca aaggccagat actcggccgc ggtcagcgcc acccgcgggg
     1141 cggcaatccg ctcgatggcc gggtcgttgg ccagattgat aaacagcacc gcccgctcga
     1201 tggctccggt gttggtcagg tcgttgatga agaactgggc ggcctcgaag gtgatgccca
     1261 ttgccgcaaa caccacggca aacgcctcgc ccttgccgcg caccgtggcc tgacgggcca
     1321 gctgggctgt gatttcatcg tgcggaagac cggcgccgga gaaaatcggc agcttctgtc
     1381 cccgcaccag cggattcagc ccgtcaatgg tgctgatgcc agtctggatg aattcattcg
     1441 gatagtcgcg ggcgtacgga ttgatggggt tgccgttgat gttgagcttc atcttcggca
     1501 gaatccgcgg gccgccgtcg atggggacgc cggtgccgct gaagacccgt ccgaggatgt
     1561 cccgcgaaac ggccatctcc atcggcttgc ccagaaaacg cactgtggtg ccgctgacat
     1621 tgatgctttc ggtgccttcg aagacctgca ccagcacctt gtcgccttcg acctgcagaa
     1681 tctggccgtg ccgcatcgaa ccgtcggcca gctcgatatc gacaatatgg ccgtatttgg
     1741 cattgtcgac cccttcgacc atcatcaacg ggccggaaat ctgggaaacc gttttatact
     1801 ctttcagcat gtctgactcc tctactgcac taatccgctt attccgctgc ggcggccaag
     1861 agttccttga cctgccggtc aatcacatcc cgaatagccg ccacctgctc aattttgtca
     1921 ttatccagat atttggcgcg ggcgatagcc tcacgcaccg gcagcttgaa caggccggcg
     1981 gtgtctgcgc cgctgtcgat ggccgccaga ccggcctgat ggaaatgcag aatcagctta
     2041 atcatctcat actgcttttc aatcggcgta taggtatcca cttcgtggaa ggcattctga
     2101 tgcaggaaat cctcccggat gctcttggcg gtttccagcg tcatccggtc gcgggcgctg
     2161 atggactcgg cgccgaccag acggacaatt tccaccagct gggcttcctg ctccagcagg
     2221 gtcatcgcct gctgcatcat ctcggcaaat tcccgcccca tctgcttgtc tttgaaggac
     2281 tcgggcaggt actgcttgta gaaggagtag ctggtcagcc agtcaatcgc cggaaagtgg
     2341 cgctgctggg ccagccggcc ctgcagagac cagaagacct tgaccacatg cagcgtggcc
     2401 tgcaccaccg tatcggacaa gtcgccgccg ggcgggctga cggctccaat caccgacagc
     2461 gccccttcgc gctgggggct tccggcgcag accacccggc cggcacgctc atagaacgag
     2521 gcgatgcggg cgcccagata agccgggtag ccctcttcgg cgggcatttc ctccagccgc
     2581 gtggagattt cgcgcatggc ttcggcccag cggctggtgc tgtcggccat cagtgccacc
     2641 gaatagccca tatcgcgata gtactcggca atggtgatgc cggtatagac cgacgcctcg
     2701 cgggcggcca ccggcatatc ggaggtgttg gcaatcagca ccacccgctt catcagcggt
     2761 tggccgctgt gggggtcggt cagttcggga aactccatca gcacatcggt catttcgttg
     2821 ccgcgctcgc cgcagccgac atagacaatc acatcggcat ccacccactt ggccagctgg
     2881 tgctggacga cggttttgcc ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnntcacatc ggcatccacc cacttggcca gctggtgctg gacgacggtt ttgccggtgc
     3061 cgaacgggcc gggcacacag gcggtgccgc cgcgggcaat cgggaagaag gcatcgatga
     3121 cgcgctggcc ggtcagcagg acatgattgg ggttgagccg ctggcggatg gcacgcggct
     3181 ggcggacggg ccatttctgc atcagtttca gttcggtttt cgtcccgtcg gcggcggtga
     3241 ctgtggcgat gggctgatcc accgtatagt cgccttcgct caagccgtca atccggccgg
     3301 acacgccggg cggaaccata atcttgtgga gcaccagcgt ggattcctga acctgaccga
     3361 tgatgtcgcc ggactggacg tcggtgccgc cggagactgt gggggtaaaa tgccagcggg
     3421 tttggcgctg cagaccgggg atggcgctgc cgcgcttgat gaagctgcct tgggcggccg
     3481 ccagctggtc cagcggacgc tggatgccgt cgtagatgct cgatatcagc ccggggccca
     3541 gctcgacgct caggggggca ccggtattga cgacttcttc gcccggacca atgccgatgg
     3601 tgtcttcgta cacctgaatg ctggcacggt cgccgtgcag tcccacgatt tcgccaatca
     3661 ggttctcgcg gcccacccgc accacatcgt acatgcgggc gccgagcatg ccttcggcta
     3721 ccacaactgg tcctgctacc ttgataattc tgccgttgtg tacattcgtc attgcactgc
     3781 ctttgtgaat gaaacagtcc gcttgtttgg tttagttttt cagaatatta atgcccgttg
     3841 ccattttcag catccggccg agggactcgg tggcgatgcc tgtcggctcg gttgtaaacg
     3901 gcacgacaat cacgcagggc atggcggcct tctggacggc ttcaaacacg ccctgcgctg
     3961 cctgcgccac attctcagcc acgaccacca gcgcatattt atcccgcaga atacgggcgg
     4021 cctgcgcctg cactgcggcc gcatcggcct ccaccgcaaa ggtgtcaagg cccattgccg
     4081 caaacggcat cacaaaatcc gcactgccta aaacggctgc tttgccttcc atcatcatcc
     4141 gctgtttcct tgtctgctta cggcatccat tcgcccagcc ggtccagaat caattttgcc
     4201 gcaagaccgt ttttcttgcc ggtcaagacc atccgcacgg tgcgaatctc ggcctctttc
     4261 atcagaaagt aggcaatcac cggctgcggt ccggcgggga tgctgcgggt ggtcttgaga
     4321 aatcccttga catagtcttc gcattccttt tccagcttca gaaacgactg ctcgctgcgc
     4381 agatagggaa tcgaagtttc cagcagctca taatacggcg tggcataaaa gccggcggcg
     4441 acggcttccc agccggcggc aagcccctgc acaaaccggt cggtctccac aaagccgggc
     4501 ggcagaaaca gccgcgtgtc ttcttcccgt ccggccattt tcagccgcag catcatccgc
     4561 agattgtcca aatcgttgcg gatccgcatc agtgacaggc agaagacatc cttcagcgcc
     4621 tcggcctgcc gaatccgcca tgccgcatac atccggtcaa tttcataatc aatccgccga
     4681 atatcgcggt tttcatagta gcccagaatg gccgcctcca ccgcttcctg cagatagtcg
     4741 ggcagccgct cgtaattttc ctgtgcaaag atatcctcaa attcctcggc cggcacattg
     4801 ccgtcgttgc tgtaatccag ccccagcggc cgttccgtaa cgacccgccg gacagccagc
     4861 cgcatattgg caaaatcctc ccgggcgcgg agaaaggtga gaatgccctc atccagtatc
     4921 agctggctga aaaggcggcg cacctcgctg cggcgctcca gcagcatctg ctccagccgg
     4981 gcggcatcgg tctgagcatc gacggcatac tcggttccgg ccagcagttc ggccgcatcc
     5041 gcaaaggaag cggcattggc catatcggca aagacgctgc gcggcagcat ggccagctcc
     5101 agcgcgcgga cccgagcggt cggataggca tagcgccaat cttcctgtcc gatgggcgga
     5161 tagcggtaaa aactgatgtc gctgccgatg gtcacgtgtc tgctcctgtc gggattttaa
     5221 tccgtgccga aaagcatgcc ggccagttcc atttccattg cgtcgcgcag ccggtccacc
     5281 agcacctccg cgctggcatt gatctgcacg cgctggcggc ggaggataaa gccgccttcg
     5341 aaatcgcccc gctgcgggca cagccgcagg cggccgcggt ccggccccag ccggctgttg
     5401 accatcgcca gaaacggctc atggatgcgg gtttcgcccc ggccgatcac cacttcctca
     5461 tcgcctgttt cgacggcttt tgtcatcagg tctgccatca gcgcctgata gggcgcatcg
     5521 ggcatctgac agagggcttg gcgggctttg gcgaagacct catccagaat tgccgtgcgt
     5581 gcggccagca gacgccgggc attgtccatc cgcgccgctg ccagcatccg ctgccagcga
     5641 tcctgaccgg ccgcttcagc cagctgttct gtcttcctgt caaactcggc aagctcggct
     5701 tccagctggg catcggcttg ggcggccttc tgacgggcct gtcccagaat ggcctccgcc
     5761 tcggcacggg cctgcgagag gattttttcg actacctgtt ctgcattcat tggtattggt
     5821 ccaatcgtac aaacggcttg ctgcgttttg ttagccgact ttaatgccca tcaggagaat
     5881 cagcagcgaa atcagaaagc ccagaatcgc atacagttca atcatcgccg catacaccac
     5941 gccggcctta aaagccattt ccgggcgctt ggcggtcatc agaatgccgg cggcgccggt
     6001 gcgcccctga tgaatggcgc tgaacagacc gccgaaggcc accggcagac aggcggccaa
     6061 aatctccagc ccctgatgcc aggccacggg atttacgctg ccgccgaaca tattgagctt
     6121 aatcatcaca aacagaccga cgacaaatcc gtagatgccc tgcgtgctgg gcagcaccac
     6181 cagcagcatc agcgttccgt agcgttcggg cttttcgctg agcacgcctg tggcgctttt
     6241 gctggcagcc gccagaccca ccgcagaccc aatgccggcc agaaagaccg ccagtgccgc
     6301 tcctgtcaaa gcaaacgtca aacccatcat ttccagcata attcttttcc tttcttgttc
     6361 ttgtcttttc tgcaggaatt attctttcag gtcaatatac cggtactgcc ggcgcagggg
     6421 ggtaaattcc tgaccgccgc cgacgaagaa tttggggaag aactccacaa actgcagccg
     6481 caggctgtga acaaaagccc ccagcattga catcgccaga ttaaacagat gcccgccgac
     6541 aaacaaaact gctcccagca gccatccgac atacggaaca tcggcgacca gcttgaccag
     6601 cacattgatg gccagaccga agcccgaacc gaccattccc agcgccatca gccggacata
     6661 actgagtata tcgcccatat agaaaacggt gctgaaaagc tgaaaaacgc cgaatccgac
     6721 gcgaccgccc cagcccattc cgcgtccgct gaacagcaga atcaaaaccg ccggcagcag
     6781 ggcggccaaa ccaaccggct tggccagacc ggccggcagc agttcgcctt tggacaggcc
     6841 cagcaccacc agacaattga gcatcacaat ccaggtcagc tggtcaaaaa ccgccgccgc
     6901 gatgtccttc ttgagcagat tggcaaagaa ggcaataaac aggccgaact gtatctgcag
     6961 atagcccacc cccagcgaca atgcaaaaaa cgtcatcggc tgggtcatcg gatcaaagag
     7021 cataatcttg cagcgcatcc agttgagtgc gctgccgaca gccgactgag ccggcagcag
     7081 ggcggtaacc gcatcgccga accagcttcc ggtaatggcg ccggccagaa aagtcgtaaa
     7141 gccgcagacc agcagcatcc aaaagacgcc tttgtcgccg cgggtctttt tcaaaatcca
     7201 cgccagcagg cccatcataa tcagcccgta gccggcatcg gccagacaca agccaaaaaa
     7261 aacagcaaaa aacggggcca aaaacaccgt cgggtccaca gagctgggca gcggcattcc
     7321 atacaagcgg gtaatgacct caaagggacg aaccgcccgg ttattgtcga tttcaatggg
     7381 aatttcttcg ccctctgccg gctcgatggg ggctatgtcg cagccgtcaa actgaccgac
     7441 cagcgcttcc agcttggggt aatccttctt
//
LOCUS       DAOTBO010000117         7413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8495_length_7413_cov_4.848192, whole genome shotgun sequence.
ACCESSION   DAOTBO010000117 DAOTBO010000000
VERSION     DAOTBO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_7413_cov_4.848192"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLE88_11075"
     CDS             <1..188
                     /locus_tag="PLE88_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77049.1"
                     /translation="SAPMDNELKIHRRRYSQVFADIGNSTPDEEYFGRREMILKRRAK
                     LETNAIEKQKGASFNDS"
     gene            complement(308..381)
                     /locus_tag="PLE88_11080"
     tRNA            complement(308..381)
                     /locus_tag="PLE88_11080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Met,seq:cat)
     gene            complement(428..856)
                     /locus_tag="PLE88_11085"
     CDS             complement(428..856)
                     /locus_tag="PLE88_11085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOM77050.1"
                     /translation="MEGQDCIFCKIIAGQIPCTKVFENEAVLAFLDIGPVSDGHLLVV
                     PKVHTSRLENTPGQTLAEAVKVLPMLAQAVQRAVDAEGCNVLCNNGRCAGQVVEHLHL
                     HLVPRKPGDGIFNSWPSFRYPPGKAEQIAQKIIQNLKPSF"
     gene            complement(914..1318)
                     /gene="rplQ"
                     /locus_tag="PLE88_11090"
     CDS             complement(914..1318)
                     /gene="rplQ"
                     /locus_tag="PLE88_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOM77051.1"
                     /translation="MAGRHLSRTSEHRLALRRNLTASLFEHETISTTLEKAKEVRSFA
                     EKLITLAKKGTLPARRRAIALLNNRAIYAQEEGQLVKKGTVIGKLFSDIGPRYLDRAG
                     GYTRIIKLPKRRLGDGGQLVLLQLVGSEKEKS"
     gene            complement(1372..2373)
                     /locus_tag="PLE88_11095"
     CDS             complement(1372..2373)
                     /locus_tag="PLE88_11095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOM77052.1"
                     /translation="MRITWRGLELPTRLEKDEQVSAGNYGRFYIEPFERGFGTTIGNS
                     LRRVLLSSLEGSAVESIKIAGVDHEFASIKGVMEDVTNIVLNVKQLVVRLQDAGPKTM
                     TVKASKAGPVTAGMIECDSSIQIFNKDLVLATLTEDINFEMEMVVGNGRGYSPAAERI
                     AQADRFEQEHGRIDIDAIYSPVVRVRYKTEDTRVGQRTNYDKLILEIWTDGTVTPEMA
                     LVEAAKILRKYINPFIQYTQAGEETVEEEVRTEEAPAGGVDEALAAKLAMPIESLQLT
                     VRSSNCLEANHIQTVGQLVKMTEADLLKIRSFGKTSLREVRRKLSELGLSLGMTDTQ"
     gene            complement(2480..3112)
                     /gene="rpsD"
                     /locus_tag="PLE88_11100"
     CDS             complement(2480..3112)
                     /gene="rpsD"
                     /locus_tag="PLE88_11100"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOM77053.1"
                     /translation="MSRYLGPSCRLCRREGMKLMLKGTRCETAKCAIEKRERSMAPGQ
                     HGWRRGRKSDYAVRLREKQKVKRYYGLLERQFNAYFRQAEKGTGNTGQDLLILLERRL
                     DNVIYKLNFAPSRKAARQLITHGHIYLNGRRVDVPDMLVKIGDKITLKNSDKTRKLIK
                     ERLDSNPNFAVQGWLQLDASKPAAAVVGMPTRDDVQLAVEEQLVVEFCSR"
     gene            complement(3166..3546)
                     /gene="rpsK"
                     /locus_tag="PLE88_11105"
     CDS             complement(3166..3546)
                     /gene="rpsK"
                     /locus_tag="PLE88_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOM77054.1"
                     /translation="MAKGIKKKVRKNVVRGIVHIKASFNNTLVTISDADGDVICQDSG
                     GTVGFKGSRKSTPFAAQRAAEKCAKTAMRYGLREVEIRVKGPGSGRESAISAIQQAGL
                     RIASIEDVTPIPHNGCRPRKRRRV"
     gene            complement(3597..3980)
                     /gene="rpsM"
                     /locus_tag="PLE88_11110"
     CDS             complement(3597..3980)
                     /gene="rpsM"
                     /locus_tag="PLE88_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOM77055.1"
                     /translation="MPRIVGVDVPNNKPAWIALTYIHGIGVYTAKKILDEAGVDGAVR
                     AGKLTEDEVSRITQIIDRDYEVEGALRRRVSQNIARLKDIACYRGIRHRRGLPVRGQQ
                     TQSNARTRKGKKKTVAGKKGVKELR"
     gene            complement(4022..4138)
                     /gene="rpmJ"
                     /locus_tag="PLE88_11115"
     CDS             complement(4022..4138)
                     /gene="rpmJ"
                     /locus_tag="PLE88_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOM77056.1"
                     /translation="MKVRSSVKRICENCKVIRRKGVLRVICSANPRHKQRQG"
     gene            complement(4167..4382)
                     /gene="infA"
                     /locus_tag="PLE88_11120"
     CDS             complement(4167..4382)
                     /gene="infA"
                     /locus_tag="PLE88_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOM77057.1"
                     /translation="MAEKDAIRFEAKVVEALPNAMFRVELQNGHQILAHVSGKMRMHF
                     IRILPGDVVTVEMSPYDLTRGRIVLIN"
     gene            complement(4412..5194)
                     /gene="map"
                     /locus_tag="PLE88_11125"
     CDS             complement(4412..5194)
                     /gene="map"
                     /locus_tag="PLE88_11125"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOM77058.1"
                     /translation="MPITLRSSREIELIRKAGIVVANVLSKLKEYAKPGVSTAELDDL
                     AMEMTHQAGAVALFKGVPCPYKNRPFPGGICTSINEQLVHGIPSPSVILKEGDILSID
                     FGVRLNGYCGDAAITVGIGSIAPERQRLLDVTSRLLQVAVEYMAPGVKWSMIARMMED
                     IARQAKFSIVRDYVGHGIGTEMHEDPKVPNFVSPELLHNDILLREGMVLAVEPMVNMG
                     TAAVKTLSDGWTVVTKDKKCSAHFEHTIAVVSGGCRVLTALP"
     gene            complement(5202..5858)
                     /locus_tag="PLE88_11130"
     CDS             complement(5202..5858)
                     /locus_tag="PLE88_11130"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOM77059.1"
                     /translation="MKLILLGPPGAGKGTQCKRIVDRYGLTHLSSGDILRAERAAGSE
                     LGRKAQSFMDSGALVPDDLIIDMMIAAIKKAPKGYVLDGFPRTVVQGEGLDKALKKAG
                     EKIDAVVNLVVPDKAIMSRMTGRRSCPKCGAVYHIENLKPKNEGQCDKGCGPLVQRPD
                     DAPAVVANRLKTYHEQTAAVVGFYVKKGGTILDIDADRTIDAVTADLFKKLDALNKVS
                     "
     gene            complement(5855..7216)
                     /gene="secY"
                     /locus_tag="PLE88_11135"
     CDS             complement(5855..7216)
                     /gene="secY"
                     /locus_tag="PLE88_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOM77060.1"
                     /translation="MLGTFVNIFKVPDLRNKILFTLALLCIYRIGFHIPVPGFDSAKI
                     SQVAEERSEESPLGRAADYLQMFTGGTLQHSSLFGLGIMPYISASIILMLMGEIIPAL
                     RKLRQEGQTGYKKIQEYTRYLTVPLCLIQALMYMKMFTGGEYIYPGMKAQAILMGVIG
                     MTAGTIFLMWLGEQIDEYGIGNGISLIIMAGILARMPWAVMQVWQNTELKVGVAAGKY
                     GPGTLVFLIAAFIFVVAGAILITQGQRRIPIQQAKQMRGHRMYGGARHYLPLRVNHGG
                     VMPIIFASSLMMFPPIVLEQIAKIPALAGSSVLRQITDAFGPTAFTYNVTFIVLIFLF
                     SYFWTTVQFQPKDMAKNLRDRGSFIPGLRPGHRTAEYLETVMIRITFFGAAFLAIIAV
                     VPSLVTQLLGVSFAVASFLGGTGLLIVVSVSLDLVQRIEANLIMRNYEGFSETGRIKG
                     AFR"
BASE COUNT         1627 a         2198 c         1830 g         1758 t
ORIGIN      
        1 agtctgcgcc tatggataat gagctaaaaa tccataggcg caggtattca caggtatttg
       61 cagatatagg caacagcacc cccgatgagg agtatttcgg acggcgggag atgattttga
      121 aacgcagggc caagttggag acaaatgcaa tcgaaaaaca gaagggtgct tcattcaacg
      181 attcatgaaa ccgcaagcca aaaccgtaca aataaagact taaaattgtc tcatttcggc
      241 tgaagatgta cagccagcga atgaaaaagg gacaagactc attgcttgtc cctgcgtgcg
      301 ttatgaatgg cgggggcagg attcgaacct gcgacctccg ggttatgagc ccgacgagct
      361 accagactgc tctaccccgc gatcaataga ctgctttata acacaacaag ccgcacttat
      421 caagagtcta aaatgacggc ttgagatttt ggataatttt ttgtgcaatt tgttcggctt
      481 tcccgggcgg atagcggaag gaaggccacg aattgaaaat gccgtcgccg ggcttgcgcg
      541 gcacaagatg cagatgcaga tgctccacaa cctgcccggc acagcggccg ttattgcaca
      601 ggacattgca gccttcggca tcgacagccc gctgaacggc ctgtgccagc atcggcagca
      661 ctttgacggc ttctgccagc gtttggccgg gtgtattttc cagccgggat gtatgcactt
      721 tgggaaccac cagcagatgg ccgtcgctga ccggacctat gtccagaaaa gccagcaccg
      781 cctcattttc aaagactttg gtacagggaa tctgtccagc aataattttg cagaaaatac
      841 agtcctgacc ttccattgtg ccgctgactc ctttgggccg attacaaacg gcctttccaa
      901 aacgctgccg tttttacgac ttttcttttt cggagccgac caattgcaga agcaccagct
      961 gcccgccgtc gccaagccgc cgcttgggca gcttgataat gcgggtgtag ccgccggccc
     1021 gatccagata ccgcgggccg atgtcgctga acagcttgcc gatgaccgtg ccctttttga
     1081 ccagctggcc ttcttcttgg gcataaatag cccggttgtt cagcagggca atggcccggc
     1141 ggcgtgccgg caaggtcccc tttttggcca gcgtaatcag tttttccgca aagctgcgaa
     1201 cttctttggc cttttccaag gtcgtcgaga ttgtctcatg ctcaaacagc gaagccgtca
     1261 gattgcggcg cagcgcaaga cggtgctcac tcgtacgact taaatgacgt ccagccactc
     1321 gatgacgcat attgttctac ccttttgtct ctgtatttcc attgactctg tttactgtgt
     1381 atcggtcatt cccaaagaca gccccagctc cgacagcttg cggcggactt cgcggagact
     1441 ggtcttgccg aagctgcgaa tcttcaggag gtccgcttcg gtcatcttga ccagctgtcc
     1501 gacggtttgg atatgattgg cctccaggca gttgctcgag cgcaccgtca actgcagcga
     1561 ctcaatcggc attgccagct tggccgccag cgcctcatcc acgccgcctg cgggggcttc
     1621 ttcggtgcga acctcttctt caacggtttc ttcgccggcc tgcgtgtact gaataaacgg
     1681 attgatgtac ttgcgaagaa tcttggccgc ttccaccagc gccatttccg gcgtgacggt
     1741 tccatcggtc catatttcca gaatcagctt gtcgtaattg gtgcgctgcc cgacgcgggt
     1801 gtcttcggtt ttatagcgta cccgcaccac cggcgaataa atcgcatcga tatcaatccg
     1861 gccgtgctcc tgctcgaagc ggtcggcttg agcaatccgt tcggctgccg gcgaataccc
     1921 gcggccgttg ccgacgacca tttccatctc gaagttaata tcttccgtca gcgttgccag
     1981 aacaaggtct ttgttgaaga tctgaatgga actgtcgcac tcaatcatgc cggctgtaac
     2041 agggccggcc ttggacgcct tgacggtcat ggttttcggt ccggcatcct gcagccgcac
     2101 aaccagctgc ttgacgttca ggacgatgtt ggtcacatct tccataacgc ctttgatgga
     2161 cgcgaactca tgatccacgc cggcaatctt aatcgattcc accgcactgc cttccagcga
     2221 ggaaagcagc acgcggcgca gactgttgcc gatggtcgtc ccaaagccgc gttcaaacgg
     2281 ctcaatataa aaacgtccgt agttgcccgc ggagacttgt tcgtcctttt ccaaccgcgt
     2341 gggcagttcc aatcctctcc aggtaattcg catagagtcg gcctcctgaa atcaggtgaa
     2401 tgttctcagc agcttccgtg cggcccgtat tcggtttaag ctgctatccg ggtattcacc
     2461 aaaaggttgc acattattgt taccgtgaac agaattccac aaccagctgt tcttccaccg
     2521 ccagctggac atcgtcgcgc gtcggcatcc cgacgaccgc agcggccggc ttggaagcgt
     2581 ccagctgcag ccatccctga acggcaaagt tggggttgct gtccagccgc tctttaatca
     2641 gcttgcgggt tttgtctgaa ttcttcaatg taatcttgtc gccgattttg accagcatat
     2701 ccggcacatc cacgcgccgg ccgttgaggt aaatatggcc gtgcgtaatc agctgccggg
     2761 ccgccttgcg cgacggagcg aaattgagct tgtaaatgac attatccaga cgccgctcca
     2821 gaagaatcag caggtcctga ccggtattgc cggttccctt ttcggcctgc cggaaatacg
     2881 cattaaactg ccgttcgagc aggccgtaat agcgcttgac cttctgtttt tcgcgaagac
     2941 ggacggcata atcgcttttc cgtccgcgcc gccatccgtg ctggcccggc gccatgctgc
     3001 gttcgcgctt ttcaatcgca cattttgcgg tttcgcaccg ggtgcctttg agcatcagct
     3061 tcattccttc ccggcggcaa agtctgcatg atggaccaag atatcgtgac atcgtttatc
     3121 ggttcctttt tctaaaactt actgactggt ttttcaattt tgtaattaga cacgccggcg
     3181 tttgcgcgga cggcatccgt tgtgcgggat aggcgtcaca tcttcgatgg atgcaatccg
     3241 caacccggcc tgctggatcg cagaaatggc agattcgcgg ccggagcccg gtcctttgac
     3301 ccgaatctct acttcccgaa ggccgtagcg catggccgtt ttggcacact tttcggccgc
     3361 ccgctgagcg gcaaacgggg tgctcttgcg cgagcccttg aagccgaccg ttccgcccga
     3421 atcctgacaa atcacatcgc cgtcggcgtc ggaaatggtg accaaggtgt tgttgaacga
     3481 cgccttgata tgcacaatgc ctcggactac attctttctg acttttttct taatgccttt
     3541 tgccatcttc tgtcaattcc caaaatgatt gcttgttacg ttcttccagc cgccacttaa
     3601 cgcaattctt tgacgccctt cttgccggcc acggtcttct tcttgccctt gcgtgtacgg
     3661 gcgttgctct gggtctgctg cccgcgaacc ggcagcccgc gccgatgccg tatcccgcgg
     3721 tagcaggcga tatccttcaa gcgggcaata ttctgcgaaa cccggcggcg gagcgcccct
     3781 tccacttcgt aatcgcggtc aataatctgc gtaatccggc tgacctcatc ttccgtcagc
     3841 ttgccggcac gcaccgcacc gtccacgcct gcttcgtcca gaattttttt ggctgtataa
     3901 acgccgatgc cgtggatata cgtcagcgca atccacgccg gcttgttgtt cggaacatcc
     3961 acacccacta tacgcggcat attaactcct gtacctatga acctttattt gtttttcatc
     4021 ctcagccctg acgctgcttg tggcgcggat tggccgagca aatcacgcgc agcacgcctt
     4081 tgcgccggat gactttgcag ttttcacaaa tgcgctttac agaacttctg actttcataa
     4141 gaccaccttt acggtttttt tagttatcag tttatcaata caatccgtcc gcgggtcagg
     4201 tcgtacgggc tcatttcgac cgttaccaca tcgcccggca ggatgcggat aaagtgcatc
     4261 ctcatcttcc ctgacacatg ggccaaaatc tgatgcccat tctgcagttc cacccggaac
     4321 attgcattcg gcagggcttc gacgactttt gcctcgaatc ggattgcgtc tttttctgcc
     4381 atattttcaa accgcgattt attttctgtg ctcacggcaa tgcagtcagc acacggcagc
     4441 cgccgctgac aaccgcaatc gtatgctcaa aatgcgccga gcactttttg tcttttgtca
     4501 ccaccgtcca gccgtccgac agcgtcttga cggcggccgt tcccatattg accatcggct
     4561 cgaccgccag caccatcccc tcgcggagaa ggatgtcgtt gtgcagcagc tccggactga
     4621 caaaattcgg caccttcgga tcctcatgca tttcggttcc gatgccgtgg cctacataat
     4681 cccgcacaat cgaaaacttc gcctgtctgg caatatcttc catcatgcgg gcaatcatgc
     4741 tccacttgac gccgggcgcc atatactcca ccgcaacctg cagcagccgg ctggttacat
     4801 ccagcagccg ctgccgttcc ggggcgattg agccgatgcc gaccgtaatg gcagcatcgc
     4861 cgcaatagcc gttcaggcgg acgccgaaat caatgctcaa aatatcccct tccttcagaa
     4921 tcacgcttgg cgaagggatt ccatgcacca gctgctcatt gatggacgta caaatcccgc
     4981 cgggaaacgg acgattcttg tagggacacg gcacaccctt gaacagggca accgccccgg
     5041 cctgatgcgt catttccatc gccaaatcgt ccaattcggc tgtgctgacg cccggttttg
     5101 catattcctt taattttgaa agaacattcg caacgactat tccggccttg cgaatcaatt
     5161 caatttctct gctgctgcga agcgttatcg gcattggctc atcaggaaac cttattcagg
     5221 gcatccagtt ttttgaaaag gtccgccgta acagcatcaa tcgtgcggtc ggcatcaata
     5281 tcaagaatcg tgccgccttt ctttacataa aagccgacca ccgccgccgt ctgctcatga
     5341 taggtcttca gccggttggc tacaaccgcc ggcgcatcat ccggacgctg cacaagaggc
     5401 ccgcagccct tatcacactg gccctcattc ttgggcttga ggttctcaat gtgatacacc
     5461 gccccgcact tcgggcagct ccgccggcct gtcatgcgcg acataatcgc cttatccggc
     5521 acaacaaggt tgacaacagc gtcaatcttt tcgccggctt ttttcaaggc tttgtcaagt
     5581 ccctcgccct gtacaaccgt tcgcggaaaa ccatccaaaa catatccttt cggcgctttc
     5641 ttaatcgccg caatcatcat atcaataatc aaatcatctg gaaccaaagc cccgctgtcc
     5701 ataaagcttt gggctttccg tcccaattcc gagccggccg cacgctcggc ccgcagaatg
     5761 tcgccgctgg acagatgcgt taagccgtag cggtcaacaa tgcgcttgca ttgggttcct
     5821 ttgcccgcac cgggagggcc taaaagtatc aatttcatcg gaaagccccc ttgatgcgtc
     5881 cggtttcgct gaatccttca taattccgca taatcaggtt tgcttcgatg cgctggacta
     5941 aatccagaga aacgctgacc acaatcagca aacccgtgcc ccctaaaaag gacgccaccg
     6001 caaaggacac acccagcaat tgcgtaacca gactcggaac caccgcaatg atggccaaaa
     6061 aggcagcgcc gaagaatgta atccgtatca tgacggtttc taaatactcg gcggtgcgat
     6121 gccccggacg cagaccgggg ataaagctgc ctctgtcgcg gaggtttttg gccatatctt
     6181 ttggctggaa ttgcaccgtt gtccagaaat acgagaacag gaaaatcagg acaataaagg
     6241 tcacattata agtaaaagcc gtcggcccga acgcatcggt aatctgccgc aatacgctgg
     6301 aaccggcaag ggccggaatc ttggctatct gctcaagcac aatcggcgga aacatcatca
     6361 agctggaagc aaaaatgatg ggcatcacgc cgccgtgatt gacccgcagg ggcagataat
     6421 gccgtgcgcc gccgtacatg cgatgcccgc gcatctgctt ggcctgctga atgggaattc
     6481 gccgctgccc ttgcgtaatc agaatagcgc cggccacaac aaagataaag gctgcaatca
     6541 aaaacaccag cgttccgggg ccgtatttgc cggctgcaac gccgactttg agctctgtat
     6601 tctgccagac ctgcatcacg gcccagggca tgcgggccag aatgccggcc ataataatca
     6661 aactgatgcc gttgccgatg ccgtactcgt caatctgctc gcccagccac atcaaaaaga
     6721 tggttccggc cgtcatccca atgacgccca tcaggatggc ctgcgccttc attccgggat
     6781 aaatgtactc gccgccggtg aacatcttca tatacatcag cgcctggata agacacaacg
     6841 gcaccgtcag atagcgggtg tattcctgaa tcttcttata gccggtctgc ccttcctgcc
     6901 gcagcttgcg cagcgctgga ataatctcgc ccatcagcat cagaataatg gaggcggaaa
     6961 tatacggcat gattcccaga ccgaacaaac tggaatgctg cagggttccg ccggtaaaca
     7021 tctggagata atcggccgca cgtcccagcg gggattcttc cgaccgctct tcggccactt
     7081 ggctgatttt ggccgaatca aaaccgggaa cggggatatg aaagccgatc cggtaaatac
     7141 acagcagggc cagcgtaaac aaaatcttgt ttcgcagatc cggaaccttg aagatattaa
     7201 caaatgtccc cagcatagtt tttccttccg tgtttgggca tcaggcaact actttgaccg
     7261 aaccgccgca gccggaaatt ttctcctgtg cactcttgct gaatttatgt gctgtcacat
     7321 tcaatttctt ggtcagctcc ccgtcgccga gaatcttcac ccggctcttg cgggaaccga
     7381 caaggccggc ctcgaccagc tcattgagac cca
//
LOCUS       DAOTBO010000118         7298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8662_length_7298_cov_4.763634, whole genome shotgun sequence.
ACCESSION   DAOTBO010000118 DAOTBO010000000
VERSION     DAOTBO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_7298_cov_4.763634"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2563
                     /locus_tag="PLE88_11140"
     CDS             <1..2563
                     /locus_tag="PLE88_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM77061.1"
                     /translation="EGMMHKNRLSCYVGTTQKSRCAVCSIFLLFLILSSVCQPVWARN
                     PYKTNFMTAYPSATNSILDKVAGTSHCGLCHYAFGGGGPRNPYGLAIEPTNKSAAAIL
                     ALGNLDSDGDGYSNNDEIRDLGLPLYINTPTFPGLTASNISQVTGVVIANIQNRLTPS
                     TGADTTPPSVTVLTPNGGQSLTANTETVIEWTAQDAGGIAAVVIYLSLDNGATFSILA
                     SGIPNTGTYIWFPANRPSNQALIRIEVSDNAFNTAYDDSDAVFAIVSPAGGRVPTTLR
                     DFDMPGTQPFEAGTLNNPSNCAACHGGYNPAVEPYYNWQGSMMAQASLDPLFKANMVI
                     ANQDAPDSGDLCLRCHLPAGWLQGRSVPTDGRAMLEADQIGVACDLCHRMLDPVYKDG
                     ISPVEDIDILALLGFPASEYALGMYTVDPAGGARRGPFVNAATGHPVIVSPFHQEAAF
                     CGTCHDVSNPVFEKDGQGGYAPNAWNTTTAADKFGSVNLAPVERTYSEWLNSAYNTPA
                     GVFAPQFGGNKQFVATCQDCHMRDVTGQGCNMNPPVRSDLPLHDMTGGSTWLPSLLPV
                     LYPGKVNSDAVASGVARARYMLQNAAQLTAAQLKETLRVTVTNETGHKLPTGYPEGRR
                     IWINVKFFDKNRSLIKESAAYDTSTGQLSHDDEAKIYEVEPAIDSRVAAATGLQEGKS
                     FHFVLNSTVAKDNRIPPRGFSNAAFAQFGGSPVAYSYPDGQYWDDTHYAVPRNAVAAQ
                     VALYYQSTSREFVEFLRDENTSNPSIGQHIYNLWNDNGKCPPELMQQVEIQLTVPADL
                     DRSFHVDLADLISLAEYWLDTNCNVPENCGRADITEDGRVDISDFAAFSSYWPWP"
     gene            2870..3703
                     /locus_tag="PLE88_11145"
     CDS             2870..3703
                     /locus_tag="PLE88_11145"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77062.1"
                     /translation="MKRLCVVLLVFSLSLAAQAALGLVEDFEAFTVPINPDGQLCTGV
                     LGGFWDTESEATGNTSIEAKDGSNVIRFMTTSGTGSVSGRGVAFAGIDKPIENDSSGT
                     LFFRFTIRTDTQVVRSYFGMHTRTGTNPLDSTRTNNPAGAIAAGFAALDNGQGGYDIK
                     TTDGATTLLAGLARSQWYNVWIVADNVNDIFDLYISEAAGPGSGVPTATPDIAYLIAD
                     DLPFGFSTTSALTGGMFVCPAATDGQSSRTYIDDIYWEIPEPATLTLLGIGILGLLRR
                     R"
     gene            complement(3775..6606)
                     /locus_tag="PLE88_11150"
     CDS             complement(3775..6606)
                     /locus_tag="PLE88_11150"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOM77063.1"
                     /translation="MKRTSVFCMLLAALTAGICWGMANRTAAPAPEQNGGLFDYRQIT
                     LDNGLTVITLEDFSCPIVSVQVWYKVGSKDERPDRQGYAHMFEHMMFKGTDRVSEKDH
                     FELIRRAGGVCNAYTSYDQTVYFETLPADQIELALWLEAERMSFLKIDQNAFDTERKV
                     VEEELRMRQNQPYGNVFKKIVASVFSVHPYRWTPIGNLAHLRATSVADLRRFWMTYYV
                     PNNATLIITGAIRHQDAQELAQRYFGWIAPGQTPPQVAVQEPQPEAKTVVIDDENAPA
                     GQVMMVWRTVPAGSRDETVLDFLSQILGTGFSSRLYRTLVAETQAAVEANTWTYNLQQ
                     DGLFAAEATLKPDSQDYDGLLDALDKQIELVRTGSISDAELEKARNQLLKELVTSNLE
                     VESKAAMLGRAAVTIGDTSRINTLMAEIRSVTKEDIQRAAQQYLTPQRGVRFIIRQNQ
                     GMERASKDDETAAVTAEPELTAPAPGRPGLQRPASFPATPPLVVKTAAAFDISCQRAR
                     LSNGLEIRVIPNHEVPFVSVMLGLTSGAWTDDKPGTASMTLAMLTRGTARRTEGQLAA
                     DLEQYGITLTGQAEMDTATVSMNCLSEHLERGMSLLSETVLEPAFDPAEFSKLMQQRM
                     TELAVRQQQPEYLANKYFRQALFGSHPYARPAEGSVADLQQLGTDDLKKWWSSFARPE
                     EAVLIFAGDVTKTQAAALAQRYLGSWRAAGAATRIELPAVPTPQSTRILLVDRPGSAQ
                     AQIQVGQLGLTRRQQPDHFIALITGNYFGGAFHSRLMDNLRVQRGLTYDARGGWRAQN
                     LAGTFEASTFTRNDAAAAAIQVLLEQIRSLQTAEPTDEEFNNTRSYFIGSFVIQRETP
                     QDIARDIWLIESQKLGRDYFRKLFKTLKNATKQDCVQLARRSLDPQRLSIVVVGDAAK
                     LKETLSGIAPVEVIVPEIGLPPENTNEQR"
     gene            6779..>7298
                     /locus_tag="PLE88_11155"
     CDS             6779..>7298
                     /locus_tag="PLE88_11155"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOM77064.1"
                     /translation="MVNSASQTADQTSAAVQQPALDAGLRNAYQWLAVLTMLLDHLGY
                     LYDIAALRYVGRLSMPAYALLFAETMRTGHVRLHRLLILAIVSQPPVMYIFEDPKLNI
                     IFGFAIFAFAASAAQQRRWFAAAAGLAMMFIPVAYGWYLYVSLAVFYWIGQPWAQRGL
                     FAAATAGYVVFTH"
BASE COUNT         1554 a         2027 c         1970 g         1747 t
ORIGIN      
        1 ggagggtatg atgcataaaa accgtctatc ttgctatgtg gggacgaccc aaaaaagtcg
       61 ctgtgctgtt tgttctattt ttctgctgtt cttaatcctt tcttctgtct gccaaccagt
      121 ttgggcgagg aatccctaca aaaccaattt tatgaccgct tatccttcgg cgaccaattc
      181 gattttggat aaggtagccg gaacatccca ctgcggcctg tgtcattatg cctttggcgg
      241 cgggggaccg cgaaatccgt atggactggc tattgaacca accaataaaa gcgcagcagc
      301 cattcttgct ttgggcaatc tggattccga tggcgatggt tacagcaaca atgacgaaat
      361 acgggatttg ggcctgccgc tctatatcaa tacgcccacg tttccgggat tgactgcctc
      421 caatatcagt caggtgacgg gtgtggtgat tgcaaatatc caaaaccgtc tgacaccctc
      481 taccggagcc gatacaacgc cgccttctgt gacggtactg actcccaacg gcggccaatc
      541 tcttaccgcc aatacggaga ctgtgattga atggactgcg caggatgccg gcggtattgc
      601 cgctgttgtc atttatctgt ccttggataa cggagcgaca ttcagcatcc ttgcttccgg
      661 catacccaat acaggcactt atatctggtt tcctgccaat cgcccctcaa accaagctct
      721 tattcgtatc gaagtctctg acaatgcatt caacaccgcc tatgacgaca gcgacgcggt
      781 ttttgcaatt gtctcgcctg ccggcggccg tgttccgacg accctccgcg attttgatat
      841 gcccggaacc cagccgtttg aagcgggtac cctgaacaat ccatccaatt gcgctgcctg
      901 ccacggcggc tacaatccgg ctgtggagcc gtactataat tggcagggca gcatgatggc
      961 ccaggcctcc ttagaccctt tgtttaaggc caatatggtc attgccaatc aggatgcccc
     1021 cgattccggc gatttatgcc tgcggtgcca cctgcccgcc ggctggcttc aggggcgctc
     1081 cgtgcccacc gacggcaggg ccatgcttga ggctgaccaa atcggcgtag cctgcgactt
     1141 gtgccatcgc atgctggatc cggtttacaa ggatggaatc agccctgttg aagatatcga
     1201 tattttggca cttctcggat ttccagcttc tgaatatgcc cttgggatgt atacagtgga
     1261 cccggccggc ggcgcccgcc gcggtccctt tgtcaatgcc gctaccggcc atccagtgat
     1321 tgtttctcca tttcatcagg aggccgcatt ctgcgggacc tgccacgatg taagcaatcc
     1381 tgtttttgaa aaggatggtc agggcggcta tgcacccaac gcttggaaca cgaccacggc
     1441 tgccgacaaa tttggctctg tcaatctggc ccccgttgaa cgaacgtaca gtgaatggct
     1501 caacagcgct tacaatactc ctgccggcgt gtttgccccg cagttcggcg gaaacaagca
     1561 gtttgtggcg acctgtcagg attgccatat gcgggatgta accggacagg gctgcaatat
     1621 gaatcccccg gtccgcagcg acctgccgct gcacgatatg accggcggaa gcacttggct
     1681 gccttctctg ctgcctgttc tgtatccggg caaggtgaat tcggatgccg ttgccagcgg
     1741 ggttgcccgc gcccgctata tgcttcaaaa tgcggctcaa ttgaccgcag cacagctcaa
     1801 agagactctc cgagtgacgg tgaccaatga aaccggccat aagcttccca ccggctatcc
     1861 tgagggccgg cgtatctgga ttaatgtcaa attctttgac aaaaaccgct ccctgattaa
     1921 ggagtcggcg gcctatgata ccagcaccgg ccaattaagc catgatgacg aagcaaaaat
     1981 ctatgaggtt gaaccggcta ttgacagcag ggttgccgct gcaacgggtc ttcaagaggg
     2041 caaatcattc catttcgttc ttaacagcac ggttgccaaa gataaccgca taccgccgcg
     2101 cggcttttcc aatgcggcct ttgcccagtt cggcggaagc cccgttgcct attcctaccc
     2161 tgacgggcaa tactgggacg acacacacta tgctgtaccc cgaaatgcgg ttgcggcaca
     2221 agtagccctg tattatcaaa gtaccagcag ggaatttgta gagttccttc gcgatgaaaa
     2281 cacgtccaat ccctcgattg gccagcacat atataattta tggaacgaca acggcaagtg
     2341 tccgccggaa ctgatgcagc aggttgaaat acaattaacc gtgccggctg acttggaccg
     2401 ttctttccat gtagatttag ccgatttaat cagtttggcc gagtactggc tggatacgaa
     2461 ttgcaatgtg cccgaaaact gcggcagggc agatattaca gaggacggca gagttgatat
     2521 cagcgatttt gccgcatttt ccagctattg gccttggccg taagagttct ccagcggctc
     2581 gattttttgc cggcccgaag atgacataaa gccctgtgtg gcagcttccg cacagggctg
     2641 ctgttatttt aaagggcggg atttgttgta ttgtgtcatt gtgagtccat aaatgcttga
     2701 ataagcacat tcttttggtt tttgaaaaat ttttttattt ttccgaaatt ccgggaattc
     2761 tgcgggagaa atcaaagcgg agagagtgtc ttttctgttt atcaacaaga catatttgta
     2821 gagccggaaa ggcggaaaat gaatgtgagt tttttggagg attggaaaga tgaagagatt
     2881 gtgtgtggtt ttgctggtgt tttctttgtc gcttgccgca caggcagcac tgggtcttgt
     2941 ggaggatttt gaggcgttta ccgttcccat taatccggat ggacagctat gcacaggcgt
     3001 attaggcggt ttctgggata cggagagcga agcgacgggg aataccagca ttgaagcgaa
     3061 ggatggaagt aatgtcattc ggtttatgac aaccagcggt accggcagcg tcagcggccg
     3121 gggagtagcc tttgcgggca ttgacaagcc catcgaaaac gactcaagcg gtacgctgtt
     3181 tttccggttt actattcgta ccgatacgca ggtcgtccgc agctacttcg ggatgcacac
     3241 ccgcaccggc accaatccat tagacagcac gcgaacaaat aatcccgccg gcgccattgc
     3301 ggccggattt gcggcgctgg ataacggaca aggcggatac gatatcaaga caacggatgg
     3361 agcaactaca ctgctggcag gtttggcacg cagtcaatgg tataatgtct ggattgtggc
     3421 cgacaatgtc aacgacatct ttgatctgta tatcagtgaa gcggcaggtc cgggcagcgg
     3481 agtgccgaca gcaacgccgg atattgcgta cttgattgcc gatgaccttc ctttcggctt
     3541 ttcgacaacc agcgcactga cgggcgggat gtttgtttgc cccgcggcga cagacgggca
     3601 gtcatcgaga acttacatcg atgacattta ctgggaaatt cccgagccgg cgacgctgac
     3661 tttgctgggt atcggcatac tgggcctgct cagacgcaga taaagccttc gtttcatcat
     3721 cggcgaacag tctgtattgc atcagggaat ccctgaagag ggagtccctg ttttttatct
     3781 ttgttcattt gtgttttccg gcggcagacc gatttccgga acgatgacct ctaccggtgc
     3841 gatgccggaa agggtctctt tgagcttggc ggcatcgccg accacaacaa tcgacagccg
     3901 ctggggatca agactgcggc gggccagctg gacgcaatcc tgcttggttg cgttcttgag
     3961 tgttttaaag agtttgcgga aatagtcccg tccgagcttc tgcgattcta tcagccatat
     4021 atcccgtgca atgtcctgcg gcgtttcccg ctgaatgaca aagctgccga taaagtagct
     4081 ccgcgtgttg ttaaattcct catccgtcgg ttcagccgtt tgcagactgc gaatctgctc
     4141 cagcagaact tggatggccg cggcggcggc gtcgtttcgg gtgaatgtgg acgcctcgaa
     4201 tgtcccggcc agattctgcg cccgccaccc cccgcgcgca tcatacgtca agccgcgctg
     4261 aacacgcagg ttgtccatca ggcggctgtg gaaggcgccg ccgaaataat tgccggtaat
     4321 cagtgcgata aaatgatccg gctgctgccg gcgggtcaat cccagctggc cgacctgaat
     4381 ctgggcctgt gcgctgccgg ggcggtccac aaggagaatg cgcgtgcttt ggggcgtggg
     4441 gacagccggc agctcgatgc gtgttgcagc ccctgccgca cgccagctgc ccaaataccg
     4501 ctgggccagc gccgccgcct gcgtttttgt tacatcgccg gcaaaaatca gcacggcctc
     4561 ctccggccgg gcaaacgacg accaccattt cttcaaatcg tccgtcccca gctgctggag
     4621 atctgcaacg ctgccctccg ctgggcgggc atacggatgc gatccgaaca gcgcctgtcg
     4681 gaagtactta ttggccagat actccggctg ctgctggcgg acagcaagtt cagtcatccg
     4741 ctgctgcatc agcttggaaa actcagccgg gtcaaatgcc ggttccaaaa ccgtctcgct
     4801 cagcagggac attccgcgtt ccagatgctc cgaaaggcag ttcatgctga cggttgccgt
     4861 gtccatttct gcctgtcctg tcagcgtaat gccgtattgt tccaaatctg ctgccaattg
     4921 tccctccgtg cggcgggctg tcccccgcgt cagcatcgcc agcgtcatgg aagccgtacc
     4981 cggcttgtca tctgtccaag cgccgctggt cagtcccagc ataaccgaaa caaaaggaac
     5041 ctcatgattg gggataaccc gaatctcaag accgttggaa agccgtgccc gctggcagga
     5101 aatatcaaat gcggcggccg ttttgacaac aagcggcggc gtggccggaa aggatgccgg
     5161 acgctgcagt ccgggccgtc cgggtgccgg cgctgtcaat tccggctctg cggtaacggc
     5221 agctgtttca tcgtctttgc tggctcgctc cattccctga ttctgccgga tgataaaccg
     5281 caccccgcgc tgcggcgtca gatactgttg tgctgcccgc tgaatgtctt cctttgttac
     5341 cgaccgtatc tcggccatca gggtgttaat ccggctggtg tcgccgatgg taaccgctgc
     5401 ccgaccgagc attgcagcct tgctttccac ctccagattg gacgtcacca gctctttgag
     5461 aagctggttg cgggcctttt caagctctgc atcgctgatg ctgccggttc gaaccagctc
     5521 gatttgcttg tccagagcat ccagcaggcc gtcataatcc tgtgaatccg gcttcagggt
     5581 ggcttcagcg gcaaacaatc cgtcctgctg gagattgtaa gtccacgtat tggcctccac
     5641 tgccgcctgt gtttcggcta ccagcgtgcg gtaaagccgg ctgctgaagc ccgtgcccag
     5701 tatctgcgag agaaaatcca gcaccgtttc atcgcggctg ccggccggca ccgtccgcca
     5761 gaccatcatg acctgtccgg caggggcgtt ttcatcgtca ataaccaccg tcttggcttc
     5821 cggctgcggc tcctgcaccg ccacctgcgg cggagtctga ccgggggcaa tccacccgaa
     5881 atagcgctgg gccagctctt gggcatcctg atggcggata gcgccggtaa taatcagtgt
     5941 agcgttattg ggtacataat aggtcatcca gaaccggcga aggtctgcca cactggttgc
     6001 ccgaaggtgt gccagattgc cgatgggtgt ccaccgatac ggatgcacgc tgaacaccga
     6061 agcgacaatt ttcttaaaca cattgccgta cggctgattt tggcgcatcc gaagctcttc
     6121 ctcgacgact ttccgctcgg tgtcaaaggc gttctggtcg attttcagaa aactcatccg
     6181 ctctgcttcc agccacaaag ccagctcaat ctggtcggcc ggcagcgttt caaaataaac
     6241 ggtctggtcg tagcttgtat aggcgttgca cacgccgcct gccctgcgaa tcagttcgaa
     6301 atggtctttt tcgctgaccc ggtcagtgcc tttgaacatc atatgctcaa acatatgggc
     6361 atacccctgc cggtcgggtc tttcatcttt cgaacccacc ttgtaccaaa cctgaaccga
     6421 tacaatcgga cacgaaaaat cctctaaggt aatgacagtc agtccgttgt ccagtgtaat
     6481 ttgccgatag tcaaataggc ctccgttctg ctccggtgcc ggtgcggcag tcctgttggc
     6541 catcccccag caaatgcctg cggttaaggc ggccagcagc atacaaaata cagaagttcg
     6601 cttcataata aatctccaaa acagtggttg atattggtat ggcacttata gtcgaaatgg
     6661 acgcggctgg tcaaataaaa aaagttgaac agcagccgct tgtaggatac cataacggcc
     6721 gttaatgccg ttaatagggt atgaaaagtg actggcaatc gatagaaaag agccgtcttt
     6781 ggtaaattct gcctcacaaa cagctgacca gacatctgct gctgttcagc agccggccct
     6841 tgatgccgga ctgcgaaacg cctaccaatg gctggccgtg cttacgatgc ttctggacca
     6901 tctggggtat ctatacgaca tagcagccct ccgctatgtc ggccggcttt ccatgccggc
     6961 ttatgcgctt ctttttgccg agacgatgag aaccggccat gtgcgtctcc atcgcctgct
     7021 gattctggca attgtcagcc agccgccggt gatgtacatc tttgaagacc ccaagcttaa
     7081 tattatcttc ggttttgcca tctttgcctt tgcggcatcg gccgcacagc agcgccgctg
     7141 gtttgcagcg gcggcagggc tggcaatgat gtttattcct gtggcctacg gctggtatct
     7201 gtatgtgtcg ctggcggtct tttactggat cggccaaccg tgggcccagc gcggtctttt
     7261 tgccgccgcc acagccggct atgtcgtctt tacgcata
//
LOCUS       DAOTBO010000119         7228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8770_length_7228_cov_4.033041, whole genome shotgun sequence.
ACCESSION   DAOTBO010000119 DAOTBO010000000
VERSION     DAOTBO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8770_length_7228_cov_4.033041"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1200)
                     /locus_tag="PLE88_11160"
     CDS             complement(58..1200)
                     /locus_tag="PLE88_11160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77065.1"
                     /translation="MEKNRIKLAILESKYYGAVTSVNDLVVRLDKERFDVTFLYLSGD
                     PGRENHLQQAGYKVCYLSRSERMPVFRPDVLVKLIRFLRRNQIDLLHCHAHKATQYGA
                     IAGIFAPKVKIIAHVHGLGRFERLRRKLAFLLFAWRINRFLAVAKAVKQDILQSNWRI
                     PASKIEVLENSVDYERFASAKISKSQARQMLNIPQKAFVFGTIGRLAPMKGQQYMIEA
                     MALIKNDMPNSHLLIIGDGPLRQVLQEQVHKSRLEERVHFTGKRADVEKCIQAMDAFV
                     LSSVASEGMPRALLEAMAGGIPPIVTDLSGMAEMIDKSDLGFVVKPGDSHAVANAMIQ
                     MMRKSPQEITAMAEMGEKKVREQYSHTVVMEKLKNIYLEIVGIMDQ"
     gene            complement(1205..1936)
                     /locus_tag="PLE88_11165"
     CDS             complement(1205..1936)
                     /locus_tag="PLE88_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOM77066.1"
                     /translation="MANRIGTFLKKKIIGKNQNLISLDEPYEVMRRLMEGHSVTGIID
                     AGASRGHISKRLLRKFPQAKVYAFEPHPMYRQVLEAYAAQEPRFCPQFAALSDCQGTA
                     ELYITESAGNTSLLKPAPALSRIDPAGAAVRQTVSVPLATIDQWRSQNGSLPIQVMKF
                     DIQGYELAALKGGVHMLETSTLLVYSEVWFNPVYEGGALLGDIDGFLRNCGFVLYDIY
                     KPKYNPNGLIQWANALFVHARWVGL"
     gene            complement(1971..2780)
                     /locus_tag="PLE88_11170"
     CDS             complement(1971..2780)
                     /locus_tag="PLE88_11170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77067.1"
                     /translation="MPKAMVTICVVNYKTLDLTRLCLRSIRKYTDYPYKMLVIDNDSQ
                     DASTEYLRSLKWIQFVSRTDKTNDSSGGYAHAAALDMGLAMCDTEYFMAMHSDTFVRK
                     AGWLTQLMRYFEKDPSTACVGGGKCELVSSWGEFFKKLTDFKTFRRKLLGTPDPLGVY
                     RYYNRTVCTIYRTEVLKKENLSFLMDREKGLTVGKKLYFELVDRGYKTVELPDSIMRR
                     YVWHLAHATQVVNPNEFAIRPRTRQKTLNLINQIIACPQVQEILQDASLDA"
     gene            complement(2802..4949)
                     /locus_tag="PLE88_11175"
     CDS             complement(2802..4949)
                     /locus_tag="PLE88_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM77068.1"
                     /translation="MEKTANFSAFKRIWDYVWPQWPRLVAVLFWCVIMAGMLSFSIVT
                     MIPVLKVMMGEEGLHGWVDRQICSKRYGMEFYLPSKADLYESAETMSRQLLIIHIADK
                     GWAKQAGLEVQNWIIGVSCGGRQARAAKQMQELLAVCPVGQPVQIEVQPVQGAAKTLD
                     MPAPAKPFYADAVQWVVGHVPREETADSKFRAIVVLMIAVTAITILRCLARFYQHYLG
                     EKIVNIAIMRIREDVFRHVLYMSVGFFSTRGTSDTTSRILGDVAMCGKGIKTLLGKAV
                     REPFSAAAALAVAFKINWQLTAIFLASAPAVIAVFAILGRKIKKATKKSLVVTAHILG
                     RIQDAMNALRIVKVYNRQEYEIDRYQQANKALLKQNLRIAKVEAGINPLLDVLGMLAM
                     CGAILVGAAWVSGQYSRLQSSEFFILIGLLGVAAESVRKVSDVWNFIQQANAAAERVF
                     AVIDEPREPEVPQAFDLKPLQKQIVFENIAFTYPGSCQRALNGINLTVPAGRTVAVVG
                     PNGSGKTTLVNLIPRFFEPDSGRILIDGQDIHKATLASLREQISLVTQQVVTFNDTIA
                     NNIAYSKPSASMDEIVQAAKMAYAHEFIEPLPDGYNTLIGENNAGFSGGQLQRIIIAR
                     AILKNPKILIFDEAMSQIDADSEAKIHAALEAIMKNRTCFLIAHRFSTVISADFIVVM
                     DGGRIAAQGRHEELIAGCDLYKRLYETQLIGAV"
     gene            complement(5242..6258)
                     /gene="rfbB"
                     /locus_tag="PLE88_11180"
     CDS             complement(5242..6258)
                     /gene="rfbB"
                     /locus_tag="PLE88_11180"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOM77069.1"
                     /translation="MRTILVTGGAGFIGANFVRMVLDEHPDTRIVNLDKLTYAGNLEN
                     LAGYIDHPRHIFVRGDICDASCAAGLIDTYGVQAIINFAAESHVDRSLMEPDVFIETN
                     VKGTLNLLKIALDKKIERFIQISTDEVYGELGPEGSFTEATPLNPNSPYSASKAAADM
                     LVRSFGRSYNLPYIITRCSNNYGPFQFPEKMIPLMIHNALHDKPLPVYGDGLYVRDWL
                     YVYDHCTAVWKVLTEGRPQTVYNIGGSSEKTNLEVVGLILQRLNKPRSLITHVKDRPG
                     HDRRYAIDASKIRAELGWKPTVTFSEGLARTIDWYLANNDWLSHVVSGDYQQYYQRMY
                     ADRV"
     gene            complement(6255..7103)
                     /gene="rfbD"
                     /locus_tag="PLE88_11185"
     CDS             complement(6255..7103)
                     /gene="rfbD"
                     /locus_tag="PLE88_11185"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOM77070.1"
                     /translation="MAIQSITILGGRGMLGRDLAQAAQDCGLGVRSFDLPELDITDPQ
                     ALERAAEGADAIVNCAAFTDVEMAESRVALANQVNGYAVGRLGQIAKEAGLPVVHIST
                     DFVFDGTKDGPYAESDKPCPVNVYGSSKLLGEVLLAESGCRWCIVRVQWTYGRHGRNF
                     ITKITEAARQRSEVQVVADQVGCPTHTLDAARILCRMLMRTDFPEGLFHLAAGGCVSR
                     YEMTCYLFERLGIQTPVRPCRSADFKTAARRPLNSCFDCTKLQTLLGTPIRSWQEMLN
                     DYLEML"
BASE COUNT         1686 a         2103 c         1705 g         1734 t
ORIGIN      
        1 gccgctgcag cggctcggtt atatgttgga attatcacac tgactttcgg cattgtttta
       61 ttggtccatg attcccacta tctcgaggta aatgtttttc agcttttcca ttacaactgt
      121 atgagagtat tgttctctga ctttcttttc ccccatttct gccattgcgg ttatttcttg
      181 agggcttttc cgcatcatct gaatcattgc atttgcaaca gcgtggctgt cgccgggttt
      241 tacaacaaac cctaaatcgg atttgtcaat catttcagcc atcccagaca agtctgttac
      301 aatgggaggt attcctcccg ccattgcttc caataacgcc cgcggcatac cctcggaggc
      361 tacggatgac agcacaaagg catccatggc ctgaatacat ttttccacat ccgctcgttt
      421 tcctgtaaaa tgtactcgtt cttccagtct gcttttgtga acctgttctt gaagaacttg
      481 acgtaaaggg ccatctccaa taatgagcaa atgactgttc ggcatatcat tttttatcaa
      541 tgccatagct tctatcatat attgctggcc tttcattgga gccaaacggc caattgtgcc
      601 aaaaacaaac gctttttgag gaatattcag catctgacgg gcctgacttt ttgatatctt
      661 tgcactggca aaccgttcat aatccaccga attttcaaga acttctatct tcgacgcagg
      721 gattcgccag ttgctctgca ggatgtcttg tttgaccgcc ttagcaaccg ccagaaatcg
      781 gttaatgcgc caagcaaata aaagaaacgc cagttttcgc cgcagtcgct caaagcggcc
      841 caaaccatga acatgtgcaa tgattttcac tttgggcgcg aaaatccccg caattgcacc
      901 gtactgagtc gccttatggg catggcagtg cagcaggtca atctgatttc tccgtaaaaa
      961 acggataagc ttgacaagca catccggccg gaagacaggc atccgttcac tgcgggacag
     1021 ataacaaacc ttgtatcccg cctgctgaag gtggttttca cgccccggat cgccgcttaa
     1081 atacaagaat gtaacatcaa accgttcttt atccagccgg accaccagat cattgacgga
     1141 ggttacagca ccgtaatatt ttgattcaag gatagcaagt ttaatcctgt tcttttccat
     1201 tgacttacag ccctacccat cttgcatgaa caaacagcgc attggcccat tgaatcagac
     1261 cgtttggatt gtatttgggt ttatagatat cataaagaac aaaaccgcaa tttcgcaaaa
     1321 atccatcaat gtcgcccagc agcgccccgc cttcataaac cggattgaac caaacctcac
     1381 tgtaaaccag aagtgttgat gtttcaagca tatgcacccc accctttaag gcggctaatt
     1441 cataaccctg aatgtcaaac ttcatcacct gtatcggaag gctgccgttt tggctcctcc
     1501 attggtcaat tgttgccaaa ggaacagaga ccgtctgtct gactgccgca ccggccgggt
     1561 caattcggct cagggctgga gccggcttaa gaagagatgt attgcccgcc gactctgtga
     1621 tataaagctc ggccgtgcct tggcagtccg atagagcagc aaactgcggg caaaaacggg
     1681 gctcctgggc tgcataggcc tccagaacct gccggtacat cggatgcggc tcaaaagcat
     1741 agacctttgc ctggggaaac tttctcagca gccgcttgga gatatgaccc cggctggcgc
     1801 cggcatctat aatgccggtt acagaatgcc cctccatcag ccggcgcatc acttcatacg
     1861 gctcatccag cgatatcaga ttctgatttt tgccgattat cttctttttg agaaatgtgc
     1921 cgattcgatt ggccacaaca tccctttcaa tgatccatca tatcctttat ttacgcatcc
     1981 agcgatgcat cctgcagaat ttcctgaacc tggggacatg cgatgatttg atttatcaaa
     2041 ttcagcgttt tctgccgggt gcgggggcga atcgcaaact cgttcggatt gaccacctgc
     2101 gtggcgtggg ccaaatgcca gacataccgc ctcattatgc tgtccggaag ctcgaccgtt
     2161 ttgtagcccc gatccaccag ttcaaaatac agcttcttgc cgacggtcaa tcctttttct
     2221 ctatccatta aaaacgacaa attctccttt ttcaagacct ctgtgcggta aatcgtgcag
     2281 actgtccgat tgtaatagcg atacacgccc agcgggtctg gcgtgcccag aagttttcgc
     2341 ctgaatgtct taaaatcagt cagcttctta aaaaactcgc cccacgagct gactaattcg
     2401 cacttgcccc cgcccacaca ggccgttgat gggtcttttt caaaataccg catcaattgc
     2461 gtcagccagc cggccttgcg gacaaatgta tcggaatgca tcgccataaa atactcggta
     2521 tcacacatcg ccaatcccat atccagcgcc gccgcatggg catagccgcc ggaagaatcg
     2581 ttggttttat ctgtccggct gacaaattgt atccatttca gcgaacgcag gtattcggtg
     2641 gaagcatcct gcgaatcgtt gtcaataacc agcattttat agggatagtc cgtatatttc
     2701 cggatgctcc gcaggcacag ccgcgtcaaa tccagcgtct tatagttgac tacgcagatt
     2761 gttaccattg ccttcggcac aattgccttc tccaataccg gctaaacagc tccaatcagc
     2821 tgcgtttcgt aaagacgctt gtagagatca cagccagcta tcagctcctc gtgtcgcccc
     2881 tgcgcggcaa tccgcccgcc gtccatcacc acaataaaat cggcggaaat cactgtgctg
     2941 aagcgatggg ctatcaaaaa gcaggtgcgg tttttcataa tggcttccaa agcagcatga
     3001 attttagcct cgctgtcagc gtcaatctgg ctcatcgcct catcaaaaat caggattttg
     3061 ggatttttca gaatcgccct tgcaatgata atccgctgaa gctggccgcc ggaaaagccg
     3121 gcattgtttt cgccgataag ggtattgtag ccgtccggca gcggctcgat aaattcgtgg
     3181 gcataggcca ttttggctgc ctgaacaatc tcatccatcg aggcgctggg cttgctgtag
     3241 gcaatgttgt tggcaatcgt gtcgttgaaa gtcaccacct gctgcgttac cagactgatt
     3301 tgttcccgca gactggccag cgttgccttg tgtatatcct gtccatcaat cagaatccgc
     3361 ccgctgtcgg gctcaaagaa gcggggaatg agattgacca gcgtcgtctt gcccgagccg
     3421 ttcggtccga ccacagcaac cgtcctgccg gccggcaccg tcaggttaat gccgtttaag
     3481 gcccgctggc aagagccggg ataagtaaat gcgatattct cgaagacaat ttgcttctga
     3541 agcggcttta agtcaaacgc ttgcggcact tccggctcgc gcggctcatc gatgaccgca
     3601 aaaacccgct cggcagccgc attggcctgc tggataaagt tccacacatc cgacaccttg
     3661 cggaccgatt cggctgccac tcccagcagc ccaatcagaa taaaaaattc gctggactgc
     3721 agccggctgt actggccgct gacccacgcc gctcccacga gaatcgcccc gcacatggcc
     3781 agcatcccca gaacatccag cagcggattg atgcccgctt cgaccttggc aatccgcaga
     3841 ttctgcttca gcagcgcttt attggcctgc tggtagcggt cgatttcata ttcctgccgg
     3901 ttgtagacct tcacaatccg cagcgcattc atggcatcct gaatgcgccc caaaatatgc
     3961 gctgttacca ccagcgattt tttggtggcc ttcttaatct tccttcccag aatggcaaat
     4021 acggcaatga cggccggagc cgaagccaga aatatcgccg tcaactgcca gttaatctta
     4081 aacgctaccg ccagagctgc cgccgcggaa aacggctccc gcacggcctt gcccagcagc
     4141 gtcttaatgc ccttgccgca catcgccaca tcgcccaaaa tccggctggt ggtgtcgctt
     4201 gtgccgcgtg tcgagaagaa gccgaccgac atatacaaca catggcgaaa tacatcttcc
     4261 cgaatccgca taatggcgat attcacgatc ttttcaccca gataatgctg ataaaaccga
     4321 gccagacagc gaagaatcgt tatggccgta accgcaatca tcagaaccac aatcgccctg
     4381 aatttgctgt cggcggtttc ttcgcggggg acatggccga caacccattg caccgcatcc
     4441 gcataaaaag gcttagccgg agccggcata tccagtgttt tggcagctcc ctgtaccggc
     4501 tgcacctcaa tctgcaccgg ctggccgact ggacacactg ccagcagctc ctgcatctgc
     4561 ttggcggccc gtgcttggcg gccgccgcag gaaacgccga taatccagtt ttgaacctca
     4621 aggcctgctt gttttgccca tcctttgtcg gcaatatgta taatcagcag ctggcggctc
     4681 atcgtctcgg ccgactcata caaatctgcc ttgctgggca gatagaactc cattccatag
     4741 cgtttactgc agatttgccg gtccacccag ccgtgcagcc cttcttcgcc catcataact
     4801 ttcaggacag gaatcatcgt tacaatgctg aacgacagca ttcctgccat aatgacgcac
     4861 cagaacagca ccgctaccaa acgcggccac tgcggccaaa cataatccca aatccgctta
     4921 aaagcggaga aattcgctgt tttttccata atctcccttg tgtttataaa acggggataa
     4981 taccccatgc cacagttgtc ttcaagtcta aaatcgaccg gccttctctc caaaaagtct
     5041 gctgattggc ctgcactttc aggcgaaact ttgcttaacg gcttccttcc gtcagcaagt
     5101 ccaacagcaa atcatttcca aataccccgc aatagccgca gccgtccatc ctccaatcgg
     5161 ccgtttggtc ctgtgttgtt tctgtcaatt ccgtctgtgt cggccactgc ggggacagcc
     5221 aagcccattc cggctgtctg cttacacccg gtcggcatac atccgctggt agtactgctg
     5281 ataatcgccg gaaacaacat ggctgagcca atcattgttg gccagatacc aatcgattgt
     5341 ccgtgcaaga ccttcggaaa atgttaccgt cggcttccag cccagttctg ctctgatttt
     5401 ggaggcatca attgcatagc gtcggtcatg gccggggcgg tctttgacgt gggttatcaa
     5461 ggaacggggc ttgttcagcc gctgcagaat cagcccgacg acctccaaat ttgtcttttc
     5521 gctgctgccg ccgatattgt aaaccgtctg cgggcggcct tcggtcagca ccttccagac
     5581 agccgtgcag tggtcgtaca catacagcca gtcgcgcaca tacagcccat cgccgtagac
     5641 cggcaggggc ttgtcgtgaa gggcattgtg aatcatcagc ggtatcatct tttcaggaaa
     5701 ctgaaacggc ccgtaattgt tggaacaccg cgtaatgatg tacggcagat tgtagctgcg
     5761 gccgaaggag cggaccagca tatccgccgc cgccttgctg gccgagtagg gagaattcgg
     5821 attcagcggc gtcgcttccg taaagctgcc ctccggacct aactcgccat agacctcatc
     5881 ggtggaaatc tggataaagc gttcgatttt cttgtccagc gcaattttca gcagattgag
     5941 agttcctttg acattggtct caataaacac atccggctcc atcagagagc ggtccacatg
     6001 gctttcggcc gcaaaattga tgatcgcctg caccccgtag gtatcgatca gccctgctgc
     6061 acaggacgca tcgcagatat cgcctctgac aaaaatatgc cggggatggt caatatagcc
     6121 ggcaagattt tccagattgc cggcgtaggt cagtttgtcc agattgacaa tccgcgtatc
     6181 ggggtgctca tccagcacca tccgtacaaa attggcgccg ataaagccgg ctccgccggt
     6241 taccagtatg gtcctcatag catctccaaa taatcgttaa gcatttcctg ccaagagcgt
     6301 atcggggttc ccagcagggt ttgcagcttg gtgcaatcaa agcagctgtt aagcggccgg
     6361 cgggcagccg tcttgaaatc cgcactgcgg cacggcctga caggcgtttg aatgcccagc
     6421 cgctcaaaca gataacaagt catctcatag cggctgacgc agccgccggc ggccagatga
     6481 aacagccctt cgggaaaatc cgttcgcatc agcatccggc agagaatccg ggccgcatcc
     6541 aatgtatggg ttggacagcc cacttggtca gcgaccacct gaacctcgct gcgctggcgg
     6601 gctgcctcgg taatcttggt aatgaagttt cgtccgtgcc ggccgtaggt ccactgcacc
     6661 cgcacaatgc accagcggca gccggattcg gccagcagca cttcgcccag cagcttggaa
     6721 ctgccgtaca cattgacagg acagggcttg tcgctttcgg catagggacc atcctttgtg
     6781 ccgtcaaaaa caaaatccgt gctgatatgc accaccggca ggccggcttc ttttgcaatc
     6841 tgccccagcc ggccgacagc atacccgttg acctgattgg ccagcgccac ccggctttcg
     6901 gccatctcga catcggtaaa tgccgcacaa ttgacaatcg catctgcccc ttcagcagcc
     6961 cgctccagtg cctgcgggtc ggttatatcc agttcgggca aatcaaaact ccgcacgccc
     7021 aagccgcagt cctgcgctgc ctgcgccaaa tcacggccca gcattccccg accgccgaga
     7081 atggttatcg actggatggc catcgttcaa cctcgatacg ccggcagacg ctccggggca
     7141 atctggctca gcagcggcag gcggctgtct ttttccgaaa gaatcggctg gtcaatcggc
     7201 caatcgattc ccagcgacgg gtcattcc
//
LOCUS       DAOTBO010000120         7199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_8811_length_7199_cov_5.453108, whole genome shotgun sequence.
ACCESSION   DAOTBO010000120 DAOTBO010000000
VERSION     DAOTBO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8811_length_7199_cov_5.453108"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLE88_11190"
     CDS             <1..1133
                     /locus_tag="PLE88_11190"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOM77071.1"
                     /translation="ADDELNKKIESAFSTWTQMIGLAAKFRTMRMAKSTDGETFAILT
                     GNQNLYGPVKLDLHLVEADRVTTPYPKLLDDNTVDGIEMDNSGEPVRYNIMRFHPGDL
                     RAAINDYDVVDANAVIHWFRVDRPGQHRGVPEITPSLPLFAQLRRYTLAVLGAAETAA
                     DFAAVLFTDAPANGEAAQVEPMDIVELEKRMATVLPDGWKLGQIKAEQPATAYGEFKR
                     EILNEIARCLNLPYNIAACNSSGYNYASGRLDHQTYYKSIRVEQADMASVVLDQILAA
                     WMYEAMLSSEFSVLRTLQYLPHQWFFDGTEHVDPAKEANAQKTRLDSNTTTLAAEFAR
                     QGKDWETELQQRAKEKQLMADLGLTETQNQTATLLEDDDDEE"
     gene            1120..3078
                     /locus_tag="PLE88_11195"
     CDS             1120..3078
                     /locus_tag="PLE88_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77072.1"
                     /translation="MTKNNIPNNFHFECPLDIEAAADDKAVPKFSMVAYTGGPMKIAG
                     FTHPVVVDLEGLSIDRQNIPIRLDHNPKQGVGHTDRVVIENGQVMAEGLISRDTSWAR
                     DVIKSGKNGFPWQASIGADIIQAEFIPNGNQIQVNGRTFDGPLYVIRQSILKEISFVD
                     NGADTNTSAQVAAQSKEEKQMNDETKTTDLKAKEVTPEPAKTATCPCKAQTPEKVEAA
                     AAPDPVADMRKSIADETKRIQAIRTVCNGKHIDIEAKAIEEGWDVTKCELEVLRASRP
                     KAPAVHTQKTVHTPHIFEAVALMAAGIGNTRLEAIYDGPTLDAADKLRGIGIQEFCEQ
                     VVGMQLPRFRRDASGWLQAAFSTTSLPGILSNIANKMLLEGYNYIEDAWRQICKIASV
                     NDFKEHSRYRMTGAFKFEQVGPDGELKHGKLDEMKYGQKADTHGIMFALTRQMIINDD
                     MGAFTDIPRQIGMGAAEAIADAVWGLLLSNPNSFFSAGNKNYLDGADTALSVDSLTAA
                     EILFSGQTKPNGRPLGIGASILLVPTALKVAAQLLMSSVLLNETTTANKGKPANNPHV
                     GKFGVVSSAYLANASFAGASSKAWYLFADPNRLPALEVAFLNGVDRPTVEKTDADFNT
                     LGIQFRGYIDFGVREQDFRGAIKLKGEV"
     gene            3135..3476
                     /locus_tag="PLE88_11200"
     CDS             3135..3476
                     /locus_tag="PLE88_11200"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOM77073.1"
                     /translation="MAVAQFVQEGKSIDYTPGADVSAGDIVVQGDLLGVAQIDIAANQ
                     LGAVAVAGVFDIDKATGVGTGIAVGTKVYWDVADQQAKADDEAGANKYLGKTIVAAGD
                     DDAKVRVRLEQ"
     gene            3477..3821
                     /locus_tag="PLE88_11205"
     CDS             3477..3821
                     /locus_tag="PLE88_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77074.1"
                     /translation="MLNQGLDWLEQRLMGFCSSPVQYRRDAQTQTVDAVFGKTDIEVE
                     DQTGISVSSFVWDFLIDAQKLGFEPDVCDLIVVNGRLFEVMNLSGQGCWRWTGPNRKT
                     YRIHTKDIGDDV"
     gene            3811..4050
                     /locus_tag="PLE88_11210"
     CDS             3811..4050
                     /locus_tag="PLE88_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77075.1"
                     /translation="MTCNSEQYENVCKGEFAELHKKLDGLDEAIRGNGKPGIQLRLDR
                     LEQEKLTRSKVTWFLVGIAASVGGAVLTSLIMGWL"
     gene            4047..4475
                     /locus_tag="PLE88_11215"
     CDS             4047..4475
                     /locus_tag="PLE88_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77076.1"
                     /translation="MKLALYIADGIVAELAGNSFSEPLVIARRVLPEYEIAELKALTV
                     TVVPKSVQITNITRQSSSFEVAVDVGIQQKIGKDTDAEVARLSGVVSEIVMFMNRRKL
                     TGFPAAVFVSIANEPVYAPEHLSEKRLFTSILTVHYKVIQ"
     gene            4472..4726
                     /locus_tag="PLE88_11220"
     CDS             4472..4726
                     /locus_tag="PLE88_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77077.1"
                     /translation="MNNIIARKIELTDTYQPLAAKQTVATVTLSALPSNTNTAYLLGD
                     TGQDVPLIPGEWHTLHHVDLASIQVKGTAGDIITLNGGTW"
     gene            4726..5079
                     /locus_tag="PLE88_11225"
     CDS             4726..5079
                     /locus_tag="PLE88_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77078.1"
                     /translation="MPYRTNEIHSGMIIMWSGLLSNVPAGWSVCDGTNGTPNLRQKFI
                     RGANAEMGAQGGSASHTHNMESYIGCAQGSLYMVNGGCSCQIAAQGHTHPMYVNAQSS
                     SNIPPYYELIFIRKD"
     gene            5085..5657
                     /locus_tag="PLE88_11230"
     CDS             5085..5657
                     /locus_tag="PLE88_11230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOM77079.1"
                     /translation="MDVTVAIPTFNSACTIMSVLQRLLHQDVKPTILMIDNGSKDGTV
                     EMLQAVIDNKWFGAADIVLQTAPQLLGGREKNIPYMRHKLAQAVETEYLFFLDSDVLL
                     QGHCLLDLKEYMDEDANRKGVAVRYDPLADHVQFGAILMKTQIAKQIKWNTGEGKCEC
                     RWAVESLKEMNPDWYVTLHPDYQAMHLKGF"
     gene            5660..5827
                     /locus_tag="PLE88_11235"
     CDS             5660..5827
                     /locus_tag="PLE88_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77080.1"
                     /translation="MMKIFCDKCEQEITDPNEISAVFEDGVAKHYHKSDCFVEAREKL
                     KSVAVKPIESV"
     gene            5829..>6019
                     /locus_tag="PLE88_11240"
     CDS             5829..>6019
                     /locus_tag="PLE88_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77081.1"
                     /translation="MMKITHLFFDSPKVIRAVDKSTRKVLSRFGAFVRRTAKQSIRKR
                     KKSSTPGTPPSSHTGLLKR"
     assembly_gap    6020..6029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6030..6247
                     /locus_tag="PLE88_11245"
     CDS             <6030..6247
                     /locus_tag="PLE88_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77082.1"
                     /translation="DRQKDSVVIGPTRLTENNRGEAPSILEYGGRTTIQAKKKKTRVR
                     IRSRPFMGPAFEKEKSTLPSLWKNSIK"
     gene            6281..6709
                     /locus_tag="PLE88_11250"
     CDS             6281..6709
                     /locus_tag="PLE88_11250"
                     /inference="COORDINATES: protein motif:HMM:NF017961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HOM77083.1"
                     /translation="MNAKIYQGAAAADPATLDPATLTEMGNVKDVTLSLEAGEADITT
                     RANSGWRATAPTLRECSCEFEMVWKPGDVGFEAIKSAFLSAGTVALAVLTGAHNESGA
                     EGPVGNWSITNFSRSEALEEAVTVSVTAKLAAFGQWFEAA"
     gene            6749..>7199
                     /locus_tag="PLE88_11255"
     CDS             6749..>7199
                     /locus_tag="PLE88_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77084.1"
                     /translation="MKTFNDAAGRTWTIALNLGTAMQVKEKLNIDLLRPEDGDPPLLT
                     QLGTDEMLLGEVLCALLGPQFEKHNVTDADVRMSFDGSTLLAAQKAFYEELIDFFRSR
                     GRTDRAKAVAAQMQLIDKATNAIEAKIEAMDLDNLIDGALSGTLPDTS"
BASE COUNT         1816 a         1869 c         1973 g         1531 t
ORIGIN      
        1 cggcggatga tgaactcaat aaaaaaatcg agtccgcctt tagcacctgg acacaaatga
       61 ttggcctggc cgccaagttt cgcacgatgc ggatggctaa atcgaccgat ggcgagacat
      121 tcgccatctt aaccggcaat caaaacctat acggtcccgt gaaattagac cttcatctgg
      181 tcgaggccga ccgggtgact acaccgtatc caaaactgct ggatgataat acggtcgacg
      241 ggattgagat ggataatagc ggcgaacctg tccgctacaa catcatgcgg tttcatccgg
      301 gtgacctgcg ggcggccatc aatgattatg atgttgtcga tgccaatgcg gtcatccact
      361 ggttccgagt cgaccgtcca ggccagcacc gaggggtgcc ggaaatcaca ccatcgctgc
      421 cgttgtttgc tcaattgcgg cggtacacct tggcggtgct gggcgctgca gaaacggcgg
      481 cggattttgc ggcggtgttg tttaccgatg cacctgctaa tggcgaggcg gcccaggtcg
      541 aaccgatgga catcgtcgaa ctggaaaaac gcatggccac ggtcctgccg gacggctgga
      601 agctgggcca gatcaaggct gaacaaccgg ccacggccta tggtgaattc aaacgtgaaa
      661 tcctgaacga aatcgcccgt tgtttgaacc ttccgtacaa tatcgcggcg tgtaattcat
      721 ctggttataa ctacgcgtca ggaagattgg accaccagac ctactataag agcattcgtg
      781 tcgaacaggc ggatatggcg tcggtggttc tggaccagat tttagccgcc tggatgtatg
      841 aggcgatgct gtccagtgaa ttttcggtac tgcgaaccct ccagtatctg ccgcatcaat
      901 ggttctttga cggcaccgag catgtcgacc cggctaaaga ggccaatgcc caaaagacac
      961 gcttggattc caataccacc acgctcgctg ccgaattcgc ccgccagggc aaggactggg
     1021 aaaccgaatt gcagcagcgt gccaaagaaa aacaactcat ggccgacctg ggcctgactg
     1081 aaactcaaaa tcaaaccgcc actttattag aggacgacga tgacgaagaa taacatcccc
     1141 aataattttc attttgaatg cccgttggat atcgaagccg ctgccgacga taaggcggtt
     1201 ccgaagtttt caatggtggc ctataccggc gggccgatga agattgccgg gtttacgcat
     1261 ccggtcgtgg ttgacctgga aggcctgtcc atcgaccgcc agaacatccc gattcgcctg
     1321 gaccataatc ccaaacaagg cgtcggtcat accgaccgtg tcgtcatcga gaacggccaa
     1381 gtcatggcgg agggcttaat ttctcgcgac accagttggg cacgggatgt gattaaaagc
     1441 ggcaagaacg gcttcccgtg gcaggccagc atcggggcag acattatcca ggccgaattc
     1501 attcccaatg gcaatcagat tcaggtaaac ggcaggacct ttgacggtcc tttatatgtc
     1561 attcgacaat ccattcttaa ggagatcagc ttcgtggata acggcgcaga caccaacacg
     1621 tccgctcagg tagcggcaca atccaaagag gaaaaacaga tgaacgatga aacaaaaaca
     1681 accgacctca aggccaaaga ggtcacaccc gaaccggcta aaacagccac atgcccctgc
     1741 aaagcacaaa cgccggaaaa agtcgaagcc gcggctgcgc ccgatccggt ggcggacatg
     1801 cgtaaaagta ttgccgacga aaccaaacgg attcaggcca tccgcaccgt ctgcaacggc
     1861 aagcacatcg atatcgaggc caaggccatc gaagaaggct gggacgtaac taagtgtgaa
     1921 ctggaagtgc tgcgggcttc gcgtccaaag gcaccggccg tgcatactca aaaaacggtt
     1981 cacacgcccc atatcttcga ggcagttgcc ctcatggccg ccggaatcgg caacacccgc
     2041 ctggaggcga tctatgatgg gcccacactg gacgccgccg ataaactgcg cggcatcggc
     2101 atccaggaat tctgcgagca ggtggtcggg atgcagttgc cgcggttccg gcgggacgcc
     2161 tccggttggc ttcaggcagc gttcagtacc acctcattgc cggggattct ttccaacatc
     2221 gccaacaaga tgctgctgga aggctacaac tatatcgagg atgcctggcg tcaaatttgc
     2281 aaaatcgcct cggtcaacga ctttaaggaa cacagccgtt accggatgac cggtgcgttc
     2341 aagttcgagc aggtcggccc ggacggcgaa ctcaagcacg gcaagctcga tgagatgaag
     2401 tatggccaga aggccgacac ccatggcatc atgtttgccc tgacccggca gatgattatc
     2461 aacgatgaca tgggtgcctt cacggacatc ccgcgtcaga ttggcatggg tgcggccgag
     2521 gcgattgccg atgccgtctg gggtctcttg ctgagcaatc ccaacagctt cttttcggca
     2581 ggcaacaaaa actatctgga tggtgccgac acagcactca gcgtggattc gctgacggcc
     2641 gcagaaatcc ttttcagcgg acagaccaaa cccaacggtc ggccgctggg aattggtgcc
     2701 tcgattctgc tggtgccgac ggcgctgaag gtcgctgcac aactgttgat gagttcggtt
     2761 ctcttgaacg agactaccac cgccaacaag ggaaaacccg ccaacaatcc gcatgtcggc
     2821 aagttcggtg tcgtttccag tgcgtacctg gctaacgcct cgttcgccgg tgcgtccagc
     2881 aaggcgtggt atctgttcgc tgacccgaac cgcctgcctg cattggaagt ggcgttcctg
     2941 aacggtgtgg accgtccgac ggtggaaaaa accgatgcgg acttcaacac actggggatt
     3001 cagttccgcg gctatatcga cttcggggtt cgcgaacagg acttccgcgg ggcaatcaag
     3061 ctcaagggtg aggtgtaaac ctcatccctt aagcttgtgt ggatgataac ctttaatttc
     3121 agagaggata ttttatggca gttgcacaat tcgttcaaga aggaaaatcg attgattata
     3181 cacccggtgc tgatgtcagt gccggggaca tcgtggtgca aggtgatctg ctcggcgttg
     3241 ctcagatcga catcgccgcc aaccaactgg gggccgttgc ggtagccggt gtctttgaca
     3301 tcgacaaagc caccggggtc ggcaccggga ttgcggtggg caccaaggtc tactgggatg
     3361 tagcggatca gcaggccaag gcagatgatg aagccggtgc caataaatac ctgggcaaaa
     3421 ccatcgttgc tgctggtgat gatgacgcca aggtccgggt ccgactggag cagtaaatgc
     3481 ttaatcaagg attagattgg ctggagcagc ggctgatggg cttctgctcc agcccggtcc
     3541 agtaccggcg tgatgctcag acacaaaccg ttgatgccgt attcggcaaa acggacattg
     3601 aggtcgaaga ccaaaccggc atctcggtca gcagttttgt gtgggatttt ctaattgacg
     3661 cacagaagct gggatttgaa ccggatgtct gtgacctcat cgtggtcaac gggcgtttgt
     3721 ttgaagtgat gaacctgtcc ggtcagggct gttggcgatg gacgggaccg aaccgaaaaa
     3781 cctatcgcat ccatacgaag gatattggtg atgacgtgta acagcgaaca atatgaaaat
     3841 gtgtgtaaag gcgaattcgc cgaacttcac aaaaaactcg atggacttga tgaggccatc
     3901 cggggcaacg gcaagcccgg catccaattg cgactggacc ggctggaaca agaaaaattg
     3961 acccgcagca aggtaacctg gttcctggta gggattgccg caagcgttgg cggtgctgtg
     4021 ctgacttcgt tgattatggg gtggctatga agcttgcatt atatattgca gatgggattg
     4081 tcgccgaact ggctggcaac agctttagtg aaccgctggt aatagctcgg cgggtactgc
     4141 cggagtacga aattgccgaa ctgaaggcat taaccgtcac ggttgttccc aaatctgttc
     4201 agataaccaa catcacgcga cagtccagta gctttgaggt cgctgttgac gtcgggattc
     4261 agcagaagat cggcaaagat accgatgccg aagttgcccg tttgagcggc gtggtttctg
     4321 agattgttat gtttatgaat cgacgaaagt tgacaggttt tcctgccgcg gtgtttgttt
     4381 ccattgccaa tgagccggtc tatgctccgg aacatctgtc tgaaaaacga ctgtttacgt
     4441 ctattctgac tgttcactac aaggtgatcc aatgaacaat atcattgccc gaaaaattga
     4501 gttaaccgat acgtatcaac cgctggccgc taagcaaacg gtggctacgg taaccctgtc
     4561 ggcgctgcca tcgaatacca ataccgctta tctgttgggc gataccggtc aggatgtccc
     4621 gttgatcccc ggcgaatggc acacgctgca tcatgtggat ttggcgtcga ttcaggttaa
     4681 agggaccgcc ggtgatatca tcaccctcaa cggaggaacc tggtaatgcc gtatcgaaca
     4741 aacgaaatcc actccggcat gatcatcatg tggtccggtc tgctcagtaa tgtaccggca
     4801 ggctggagcg tttgcgatgg caccaatggc acaccgaact tgcgccaaaa attcatccga
     4861 ggcgcgaatg ctgaaatggg cgcacaaggc ggctctgcaa gtcataccca taacatggag
     4921 agttatatcg gatgtgcaca gggttccctt tatatggtca atggaggctg ctcctgtcag
     4981 atagctgctc aaggccatac ccatccgatg tatgtcaatg cccaaagttc atcgaatatc
     5041 cccccttatt atgaacttat ctttatccga aaggactaaa ttctatggat gttactgttg
     5101 cgattccaac atttaacagc gcctgcacga tcatgtctgt tctgcaacgt ctgcttcatc
     5161 aggatgtcaa accgacgatt ttaatgatcg acaacggcag caaagacggc accgtcgaga
     5221 tgctgcaggc ggttattgat aataaatggt ttggagccgc tgacatcgtg cttcaaacag
     5281 cccctcagct tttgggcgga cgcgagaaaa acatcccgta catgcgtcat aaactggccc
     5341 aggcggtcga gacggagtat ctgttttttc tggacagtga tgtgctgctg cagggtcatt
     5401 gtttgctgga tttaaaggaa tatatggatg aagacgccaa tcggaaaggc gtcgctgttc
     5461 gctatgatcc gcttgccgat catgtccaat ttggcgcgat tttaatgaaa acccagattg
     5521 ccaaacaaat caaatggaat accggcgagg gaaaatgtga atgtcgctgg gccgtcgaat
     5581 ctctcaaaga aatgaacccg gattggtatg tcaccctgca tccggattat caagcgatgc
     5641 atttgaaagg attttaacca tgatgaaaat cttctgtgac aaatgcgaac aggaaattac
     5701 agacccgaac gaaatcagcg ccgtctttga ggatggtgtt gcgaaacatt atcataagtc
     5761 tgattgtttt gtggaggccc gcgagaagct caaatccgtg gcagtcaagc ccatcgagtc
     5821 agtctaagat gatgaagatc acccatctgt tctttgacag ccccaaagtc attcgggccg
     5881 tcgataaatc cacccgaaag gttctctcga ggttcggggc ttttgtccgt cgaacggcga
     5941 aacagagcat ccgcaagcga aagaaatcgt cgacgcctgg aacgccgccg tccagtcata
     6001 caggactctt aaagcggttn nnnnnnnnna tgaccgacag aaagacagtg tcgtcatcgg
     6061 accaacccgg ctgaccgaga acaaccgtgg cgaggcaccc tccatcctgg aatacggcgg
     6121 ccgaacgacc atccaggcca aaaagaaaaa aacacgtgtg cgaatccgct cccgaccgtt
     6181 catgggaccg gcatttgaga aagaaaaatc cactcttccc tctttgtgga aaaactcaat
     6241 caaataggag actttaccaa tgccagactt tttattgggc atgaatgcca agatttacca
     6301 gggcgcagcg gcggccgacc ctgccaccct ggacccggca acgctgaccg agatgggtaa
     6361 cgtcaaagat gtaacactct cactggaagc aggcgaagcg gacattacca cccgtgccaa
     6421 cagtggatgg cgggcaaccg caccgaccct gcgggaatgt tcctgtgagt tcgagatggt
     6481 gtggaaaccc ggtgatgtgg gttttgaggc cattaaatca gcattcctgt cggccgggac
     6541 cgtcgcctta gcggttttga ccggtgcaca taacgaatcc ggagccgaag gcccggtggg
     6601 caactggtcc atcaccaact tctcgcggag tgaagccctg gaagaagccg tcaccgtcag
     6661 cgtgaccgcc aagctggccg cattcggtca gtggtttgaa gcggcgtagg tacgaatgaa
     6721 taacgaataa tgaagaaaag aggaacctat gaaaacattt aatgacgccg ctggccgcac
     6781 ctggacgatt gccctgaacc tcggtacggc catgcaggtc aaagagaagt tgaatattga
     6841 cctcttacgg cccgaagacg gcgacccacc gctattgacg cagttgggca ccgatgaaat
     6901 gctgttaggt gaggtcttgt gtgccttgct cggtccccag tttgaaaaac acaatgtaac
     6961 cgatgccgat gttcgcatgt cgtttgacgg ttctacgctc ctggccgcac agaaggcctt
     7021 ttatgaggaa ctcatcgatt ttttccgcag ccgcggccgg accgaccgag ccaaagcggt
     7081 cgcggctcag atgcagctca tcgacaaggc aaccaacgcc atcgaagcca aaatcgaagc
     7141 gatggacctg gacaacctga tcgatggtgc actatctggc acattgccgg atacctcgg
//
LOCUS       DAOTBO010000121         6909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9314_length_6909_cov_5.200613, whole genome shotgun sequence.
ACCESSION   DAOTBO010000121 DAOTBO010000000
VERSION     DAOTBO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9314_length_6909_cov_5.200613"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PLE88_11260"
     CDS             complement(<1..1735)
                     /locus_tag="PLE88_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOM77085.1"
                     /translation="MENSTFRPSICAALFLAAVWSLPAQEISDNYFDMSLEQLLDVPI
                     ISASRMEQKSTQLAIPVGVITADEIHASGLTTLPEILQLLPGVDVRRFNRSHYIVGVR
                     GMSSFTSDRTLVLIDGRNAMDPTFGAPDWLTLPVMIEDIERIEVLRGPGGAAWGANAF
                     TGVINIITKKPDNNLQSIWSTSITEFGDSYSHIRLAQSKGKWSWRLSAGYESIEDSDA
                     AGAGHTVSAFSELNFLMGFDSYKARDFSRTGRFDSVFHYRYSDLTDISFGAAWSGMET
                     GDSESVGRYPMKDALSNMTRLFARVDHEFEDGSAGWLQWYGNYAVSHQPHITSRFASY
                     DNYLEGQYTMPLTLDHSLTVGTNLRWTHLTSDNDAAAGEIMFRRGAYDEYWAGLFAID
                     RIKLSSRLSLENQIRLDRYSRTHSDWSLRSSVLYALDDNQEHVLRAGLGRAFRAAGVM
                     VREASLTGLGGLFNVVRPSEKLTNESTYALEAGYTGKLSDHLTVQVNSYYQRMEDLIG
                     SVNEYFGPIAVTHFDNIAGANTYGAETELAWKSRKLHVSGWYAYNEFVTDDNEQAIRA
                     YFPARHKAGLRT"
     gene            complement(1756..2175)
                     /locus_tag="PLE88_11265"
     CDS             complement(1756..2175)
                     /locus_tag="PLE88_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM77086.1"
                     /translation="MFDFFGLCKKSCKTKILVVDDEPNIVQTLKDRLEMNEYTVFTAA
                     NGQEGLQMAQQESPDLVLLDVIMPIMDGHEMLEQLRRQPWGRTIPVIMLTARSQAQDI
                     ARARACEIEDYIIKPFDLSELLEKIENIIERRKSLVS"
     gene            complement(2616..6746)
                     /locus_tag="PLE88_11270"
     CDS             complement(2616..6746)
                     /locus_tag="PLE88_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOM77087.1"
                     /translation="MAWLLLSAAGIAAICPQGDVSGDCRVDLDDVAVLAEQWLTEGTA
                     ANLNDDSIVNWGDFGAVSSNWGVKVSRVIISELMADNTKTIRDDENQFSDWIELYNVS
                     PTAVSLAGWYLTDNPNNLCKWALPDVEIAAGGFLIVWASGQDRREPSGPLHTNFSLKK
                     EGEYLALVEPDGVTIASEYKPKFPAQFPDISYGVSMTVCQERLIGSQTACRYSVPQNS
                     SQIGQDWAATDFDDSTWSSGVLGLGYDAGTVGTVWENVARGKPATQSSNYSASYPASN
                     AVDGIYNNFSHTASNDTNSTWQVNLGGDYYISKIILYNRTGCCWPRLRDITVTVRDAQ
                     NTQDLFVSDLLNPENILLGPMTLEVDVESLTGSVIPGGIVKVHRTPDPDNSAIGIGTD
                     TGTTGQNSLTLAEVEVWGSSNPVNLYGPLIQTNIRDEMQHRSSSVFVRIPFEVADKTR
                     YDYMELRVRYDDGFVVYLNGAPAASRNAPTASAWNSAATAEHPKLEAIAYESVNLTSL
                     IPLLRNGTNVLAVQGFNLAAEDDDFLFDAELVAGRVSAGAAGFLTQPTAGGMNGAETF
                     GAVADTKFSVNRGYYEEPFEVAITTSTAGATIYYTTDGSTPSAGSGSVYTEPIPITKT
                     TCLRAVAVKPGCLSTNVDTQTYIFLDDIIHQSASPPGYPTTWTTYTGAAYPADYEMDP
                     DIINPILSTDPNLIKDALRSLPVMSLVTDKANLFDPAIGIYANSGEFAVDLPDGSKKW
                     EVPVSMEYFDPVSGRQTQEDCGLRIQGAYFRKPGSTPKHSFRLLFKRMYGRSKLYFNL
                     FDYDDEAADSFDTLVLRAQGNDGYSWASMGGKSQYIRDEFSRRVYAAMGQAASHGTFV
                     HLYINGLYWGLYNPVERPDSSFCASYYGGKKEDWDVFKHPWLDLNEGTREAMTAMQTL
                     FQYDIPLGSAVISDSDYWKLQGCNPDGTRNPDFPVLLDVDNFIDYMLVNFWTGNQDWC
                     WNDNNYWLGRLRVGQSTGFKFFCWDTEDNLDSPRSPVTLDMIAKVYQYDVYNTQKPTI
                     ARLQNRLMTNAQYRLRFADHAHKYLFNGGLMTAQPARALYSDLANQVELAIIAESARW
                     GDQHAATPWTQTHWINERNYILNTYFPQRVDNVIAQLKAKGLYPAVDAPVFKIDGLWQ
                     HGGQVSSAAVFSMEAAGPVWFTTDLTDPRLPAGGISPSAIEYTQPFFLNQSQCIKART
                     LSGGVWSALNEATYSVGPIAAALRITEIMYHPAADPNAEFIELQNIGSAAVNLNLVRF
                     TKGIDYTFGPTVLEPLEYVVLVRDAAAFRAAYPDFSGTIGGVYGGSLSNAGETIRLAD
                     ACGGIIHEFTYKDDWYENTDGQGFSLSIRDAAGDLMLWNQKAGWRSSTVWGGTPGY"
BASE COUNT         1641 a         2002 c         1663 g         1603 t
ORIGIN      
        1 gggtccgcaa tcctgctttg tgccgggccg ggaaataggc acgaatcgcc tgctcattgt
       61 catcggttac gaactcatta taggcatacc agccggacac atgtaacttt ctgcttttcc
      121 atgccagttc ggtttctgct ccgtaggtat ttgcccctgc aatgttgtca aaatgagtaa
      181 ccgctatggg cccaaagtat tcattaaccg aaccaatcag atcttccatc cgctgataat
      241 aggaattaac ctgcacagtc aaatggtcac tcagtttgcc tgtatagccg gcttccaagg
      301 cataggtgga ttcgtttgtg agcttttcag aaggccgaac gacattaaac aggccgccta
      361 aacccgtcaa agaggcctca cgaaccatca cgccggcggc acgaaatgcc cgtcccaatc
      421 cggcccgcag cacatgctct tggttgtcgt cgagggcata caggacagac gaacggagcg
      481 accaatcgct gtgggtccgg ctgtagcgat ccagccggat ttggttttcc aacgacagcc
      541 ggctgctcag ctttatccgg tcaatagcaa acagccctgc ccagtattcg tcatacgcac
      601 ctcgccggaa cataatttca ccggcagcag cgtcattgtc cgatgtcaaa tgcgtccacc
      661 gcagattggt gccaacagtc agggaatggt ccagcgtcag aggcattgta tattggcctt
      721 cgagataatt atcgtacgaa gcaaagcggc tggtgatatg gggctggtgt gaaacagcat
      781 aatttccata ccactggagc cagccggctg aaccatcttc aaattcgtga tccacacggg
      841 caaaaagacg cgtcatattg gacagcgcat ccttcatcgg ataccgtcct acagattccg
      901 aatcacccgt ctccattccc gaccacgcag cgccgaacga aatgtctgtc aggtcggaat
      961 accgataatg aaatacagaa tcaaaacggc cggttctcga aaaatcccgg gctttataag
     1021 agtcaaagcc catcaggaaa ttcaactcgc tgaatgccga gaccgtatgg cctgcgccgg
     1081 ccgcatcgga atcttctatt gactcgtacc cggcagagag ccgccaagac catttcccct
     1141 tgctttgagc cagccggata tgcgagtagg aatcgccgaa ttctgttatt gaggtactcc
     1201 atatgctttg gagattgtta tccggctttt tggtgataat attgataaca ccggtgaacg
     1261 cattcgcccc ccaagccgcc ccgccgggac ctcggaggac ctcaatccgt tcaatatctt
     1321 ctatcataac aggcagagtc agccagtccg gcgctccaaa tgtcggatcc atcgcattcc
     1381 gcccgtcgat cagcaccagc gtccggtccg aagtgaagct gctcatcccg cgaacgccaa
     1441 caatgtaatg ggacctgtta aaccgccgaa catcgacgcc gggcagaagc tgcagaatct
     1501 ccggaagggt cgtcaagccg ctggcgtgta tttcgtcggc agtaataacg ccgaccggaa
     1561 ttgccagctg ggtacttttc tgctccatcc ttgaggctga aatgattgga acatccagca
     1621 gttgctccaa cgacatatca aaatagttat cgctgatttc ttgagctggc agggaccaaa
     1681 cagccgccaa aaacagcgcc gcacaaatgc ttggtctaaa agtactgttt tccatagagc
     1741 cggcctttgg gggaatcaac tgacaagcga cttacggcgt tcaatgatgt tttcaatttt
     1801 ttcaagaagc tcgcttaagt caaacggctt gatgatataa tcttcaattt cgcaggcacg
     1861 ggcgcgggca atatcctgag cttggctgcg ggcggtgagc ataattacag gaatcgtccg
     1921 tccccacggc tgccgtctga gctgctcaag catttcatgt ccatccataa taggcataat
     1981 gacatccagc agtaccaaat cgggagattc ctgctgtgcc atctgcagcc cttcctgacc
     2041 atttgcggcc gtaaacaccg tgtattcgtt catctccagc cggtctttga gggtttggac
     2101 aatgttcggt tcgtcatcaa ccaccaatat cttggttttg cacgatttct tacaaagtcc
     2161 gaaaaaatca aacatcgttt tgtctcccta actaaacagc accgtttcgg atatcttatg
     2221 ggctttcgcc aacacccaat agagtttact tgttcggtcg cgtttgctat cgtcatcggc
     2281 caaaaacggc cttgggtaaa cgagttttct aagggtttag gataattttt acgatgaata
     2341 acagcaaaaa cagactcaca atagatacaa tacaaatatt tgataaggac aggcattgat
     2401 ttcactgtcg ataaacgcct gcagatccgc tattttgtct gaaaaagcgg attgatttgc
     2461 cggtttccag cctacccccc aagaggttgg caaaaatgca aaaagaaaaa aatagccgtg
     2521 ttataaacag atgtgacctt cactcctcag ttatcccatt ctggacaaac tcttccggca
     2581 ccatcggcag tcacccccgc cgcactgctc catttttaat accccggtgt gccgccccag
     2641 accgtgctgg aacgccaccc tgctttttgg ttccacagca tcaggtcccc ggcagcatct
     2701 cgaatgctca gcgagaaacc ttggccgtca gtgttttcgt accagtcgtc tttgtaagta
     2761 aattcgtgaa tgatcccgcc gcaggcatcg gcaagccgga ttgtctcacc cgcattgctc
     2821 agggagccgc cgtacacacc cccaatcgtc cctgaaaagt ccggataggc tgccctaaac
     2881 gctgccgcat cccgcacaag aaccacatat tccaacggct ccagaaccgt cggcccaaat
     2941 gtataatcaa ttccctttgt aaatcgaacc agatttagat taacagccgc cgagccgata
     3001 ttctgcagtt cgataaactc ggcgttcggg tccgccgccg gatggtacat aatctctgtt
     3061 atccgcaaag cggccgctat tgggccgacc gaatacgtcg cctcattcag cgccgaccag
     3121 acacctcccg acagagtacg tgccttgatg cattgggact gattcaggaa aaacggttgg
     3181 gtgtattcaa tcgccgacgg tgaaatcccc ccagccggca gccgcgggtc tgtcaggtcg
     3241 gtcgtaaacc agaccggccc ggctgcctcc atcgaaaaca ctgctgcact gctgacctga
     3301 ccgccgtgct gccaaagccc gtcaatctta aacaccggcg catcgactgc cggataaagc
     3361 cccttggctt tcaattgcgc aataacattg tccacacgct gcggaaagta cgtattgagg
     3421 atgtagtttc gctcgtttat ccagtgtgtc tgggtccaag gcgtcgcggc atgctggtcg
     3481 ccccagcggg ccgattcggc aataatggcc agctccactt gattggctaa gtcactgtaa
     3541 agagcccgtg ccggctgggc cgtcatcagt cccccgttga acagatattt gtgcgcatgg
     3601 tcggcaaacc gcagccggta ctgggcgttg gtcatcagcc ggttctgcag ccgggcgatt
     3661 gtcggctttt gagtgttata aacatcgtac tggtaaactt tggctatcat atccagtgta
     3721 accggcgacc gcggcgaatc cagattgtct tcggtatccc agcagaagaa cttaaacccc
     3781 gtactctggc cgacccgcag ccgtccgagc cagtaattgt tgtcattcca gcaccagtcc
     3841 tgattgcccg tccagaagtt caccagcata taatcgatga agttgtcaac atccagcagc
     3901 accgggaaat cggggttgcg cgtcccatct ggattgcacc cctgaagttt ccagtaatcg
     3961 ctgtctgaaa taacagccga tcctaacggg atatcatact gaaagagcgt ctgcatcgcc
     4021 gtcatcgcct cccgcgtccc ctcattcagg tcaagccatg ggtgcttgaa gacatcccag
     4081 tcctcttttt tgccgccgta ataactggca cagaacgagc tgtccggcct ttctaccgga
     4141 ttatacagcc cccagtacag gccgttgatg tataaatgga caaatgtacc atggctggcc
     4201 gcctgcccca tagccgcata aacgcgccgg ctgaattcat cccggatata ctgcgacttg
     4261 ccgcccatcg aagcccacga ataaccgtcg ttgccctgtg cccgcaggac aagtgtatca
     4321 aagctgtcgg ctgcctcatc gtcgtaatca aaaaggttga aatacagttt cgaacgccca
     4381 tacatccgct tgaacagcag gcggaacgaa tgcttgggcg tagagccggg tttgcgaaaa
     4441 taggcaccct gaatccgcag cccgcagtct tcctgcgtct gtcttccgga aaccgggtcg
     4501 aagtactcca tagaaaccgg tacttcccat ttttttgacc cgtccggcag gtctacggca
     4561 aactcgcccg aattggcata aatcccgatg gccgggtcaa acagattggc tttgtccgtc
     4621 accagcgaca tgaccggcag cgaccgcagc gcatccttga tgaggttggg gtcagtgctg
     4681 agaatcggat tgatgatatc cgggtccatc tcataatccg ccgggtacgc agcgccggta
     4741 taggtcgtcc acgtcgtcgg ataccccggc ggactggctg actggtggat gatgtcatcg
     4801 aggaaaatat atgtctgcgt atcgacattt gttgataagc agcccggctt gaccgcaacc
     4861 gcccgcaggc aggtggtttt cgtaatgggt atcggctcgg tataaacgct gccgctgccg
     4921 gccgaaggtg tactgccgtc ggttgtataa taaatcgtcg ctccggccgt actggtggta
     4981 atggcaacct caaacggctc ttcataatag ccgcggttga cgctgaattt cgtatccgct
     5041 accgcgccaa atgtttccgc cccgttcatt ccgcccgccg tcggctgcgt gagaaatccc
     5101 gccgccccag ccgatactct gccggcgaca agctcggcat cgaacagaaa atcgtcatcc
     5161 tccgccgcca gattaaatcc ctgaacggcc agaacattgg ttccgttccg cagcaagggt
     5221 attaaagatg tcaggtttac cgactcatat gcaattgcct ccagcttggg atgttcagct
     5281 gtcgccgctg agttccacgc cgatgcagtc ggagcgttcc gcgatgcagc cggcgcacca
     5341 ttgagataaa caacaaagcc gtcgtcatac cgcacccgta gttccatata atcataacgc
     5401 gttttatcgg ccacctcaaa cggaatgcgc acaaaaaccg aactgctgcg atgctgcatt
     5461 tcatctcgaa tgtttgtttg aatcaacggg ccgtaaagat tgaccggatt gctgctgccc
     5521 cagacctcca cttccgccaa ggtcagggaa ttctggccgg tggttcccgt gtcggtgccg
     5581 atgccgattg cagaattgtc cgggtccggt gtgcgatgga ctttgacgat tccccccgga
     5641 atcaccgaac ctgtcagaga ctcaacatcc acttccagcg tcatcggccc caataggata
     5701 ttctccggat tcagcagatc cgaaacaaac aggtcctgcg tgttctgtgc gtcgcgaaca
     5761 gtcaccgtaa tatcccgcag ccgcggccag cagcatcccg tgcggttata caggataatt
     5821 ttggagatgt agtaatcgcc gccaaggttt acctgccatg ttgagtttgt gtcattgctt
     5881 gccgtatggc tgaaattgtt gtaaatccca tcgacggcat tcgaggccgg ataactggcg
     5941 ctgtaattgg agctttgtgt cgccggcttg ccgcgggcta cgttttccca taccgttccc
     6001 actgtcccgg catcatagcc cagccccaaa acgccgctgc tccacgtgct gtcgtcaaaa
     6061 tcggtcgccg cccagtcctg cccaatctgg gatgaattct gcggaacgct ataccggcat
     6121 gccgtctggc tgccaatcaa acgctcttga cagacggtca tcgaaacccc ataggaaata
     6181 tcgggaaact gtgcgggaaa tttgggcttg tattcgctgg caatcgtcac cccatccggc
     6241 tcgaccagtg ccagatattc cccctccttt ttcaaagaaa aattcgtatg cagcggccct
     6301 gacggctcac gccggtcctg tcccgatgcc cagacgatta aaaagccgcc ggcagcgatt
     6361 tcgacatcgg gcagtgccca tttgcaaaga ttgttcggat tatcggtcaa ataccagccg
     6421 gccagagata ccgctgtcgg ggatacatta tagagttcaa tccagtcgga aaactgattc
     6481 tcatcatccc gaatcgtttt cgtattgtcc gccatcaatt cgctgataat aacccggctg
     6541 actttgaccc cccaattgga tgaaaccgcc ccaaaatcgc cccaatttac gatgctgtcg
     6601 tcattcaggt tggctgctgt cccttcggta agccactgct cggccaacac cgccacatca
     6661 tccagatcaa cccggcaatc gccgctgaca tccccttgcg ggcaaatcgc cgcgatacct
     6721 gctgccgaca gcagcagcca tgccaaacaa acaaaacgat tgcaatacaa ttgttttaca
     6781 ataggccgca tccaatagca gctcacttca attcaacccg taccacatcc accagaggcg
     6841 tccgacgttc cgcaggcaca tcaaccttta gcgtattgtt gttataatgt atcttcttaa
     6901 tatttgctt
//
LOCUS       DAOTBO010000122         6878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9378_length_6878_cov_6.362597, whole genome shotgun sequence.
ACCESSION   DAOTBO010000122 DAOTBO010000000
VERSION     DAOTBO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9378_length_6878_cov_6.362597"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLE88_11275"
     CDS             <1..194
                     /locus_tag="PLE88_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77088.1"
                     /translation="NVGNPNTRNGPNPGGQSPDLCIEIFFRTKIVVYRGNIGMGFIAD
                     IMNSSIMKAFFCEYFGGGF"
     gene            complement(349..433)
                     /locus_tag="PLE88_11280"
     tRNA            complement(349..433)
                     /locus_tag="PLE88_11280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(397..399),aa:Leu,seq:gag)
     gene            566..928
                     /locus_tag="PLE88_11285"
     CDS             566..928
                     /locus_tag="PLE88_11285"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HOM77089.1"
                     /translation="MERTKLSTQCPCCGLTMKVSAMSCPACGVKVEGPFDDLILTRLS
                     AEDQRFLLDYMLSGFSIKALEENSALGYAAIRTRLDKLIANTKALMQSEEQKKAILDK
                     LRTGDINVAEAKKRLEKL"
     gene            1070..1519
                     /locus_tag="PLE88_11290"
     CDS             1070..1519
                     /locus_tag="PLE88_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77090.1"
                     /translation="MQTRPRTGFGKAALILFILLPLVPIAFNIIVGMSGSATWARLSM
                     VLAPFILLGLLCGLLAFIFGIVGWKSPAGKVAAIGVPCLGILCIPGLFLLMFIPVTHA
                     VKISHAEQSKVQDLIIHQAYPLDSLEGLITKGEAEIDEAVFSTAMGL"
     gene            1516..1863
                     /locus_tag="PLE88_11295"
     CDS             1516..1863
                     /locus_tag="PLE88_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77091.1"
                     /translation="MKIESDSPEKRTIRLFETGLMNIENRMLIYQAKLKSDLSIGKAY
                     LEMWCNIPGKGEFFSRGLEQPISGITEWATVQTPFRIEAGQMPANIKLNVVVEGTGTI
                     WIDDIKLLLCPLN"
     gene            2083..3291
                     /gene="gcvT"
                     /locus_tag="PLE88_11300"
     CDS             2083..3291
                     /gene="gcvT"
                     /locus_tag="PLE88_11300"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOM77092.1"
                     /translation="MKQTPFYSRHVALGARMVDFADWQMPIQYQQGIVAEHLTTRRDA
                     GLFDVSHMGRFRLTGPGAPKFLRYVLTNDCEKLPAGQAHYTILADNNGGAIDDAFLYR
                     PSADAYILVVNASNKDKDWQHLQMQLEHFSNVVMEDVSNSAAMLALQGPQSEAILHSL
                     APTEMLPESKRNAVGKIRIENIDLCIARTGYTGESVCFEMFIPASDASAVWDRLIDAG
                     AMPVGLGARDTLRLEAGLPLYGHELGIGKDGLEIPVFAIPQAAFAISFEDAARVFIGK
                     PALAQQAQARQRYKQSDFSNTAALPKVVRQIRLLDKGIARQGAEIFWQNRPVGWVTSG
                     TMVPYWIFRNGIRTDEYAQRAIGLCMVEPNVPIGADIEVLVRDKKLKAKIVKKNLESR
                     FGQQTAAVFE"
     gene            3346..3735
                     /gene="gcvH"
                     /locus_tag="PLE88_11305"
     CDS             3346..3735
                     /gene="gcvH"
                     /locus_tag="PLE88_11305"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOM77093.1"
                     /translation="MLIDPQARYLKTHEWAKKQTGTIFTAGLTAYAVEQLGDIVYMEL
                     PEVGKELTAGQPFGIVESVKSASDMYAPLTGRVVAANTEIPDNPDTLKQDAYDKGWLI
                     KIEAAKPAEFDELMDADAYRRFLETEA"
     gene            3800..5131
                     /gene="gcvPA"
                     /locus_tag="PLE88_11310"
     CDS             3800..5131
                     /gene="gcvPA"
                     /locus_tag="PLE88_11310"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOM77094.1"
                     /translation="MPYIANTDQQRAAMLADIGLTPEALFADVPPSLRSSGLNLPDGM
                     SEQEVRDRLAAIAAKNYIHLTLFLGGGFYDHFIPSAVYAILSRSEFYTAYTPYQPEIS
                     QGTLQAIYEFQSLICRLTEMEAANASMYDGGTALYEAMMMALRITQRRKVIIDDSVNP
                     IYRVMIQSYTQNLQIDLVETNNHEGLANREAILKALDEDTAAVMVQNPNFFGCIDDFT
                     DIAQAAHAKGALLIVSCYPISLGILKTPGAMGADIATGEGQSLGLPMSFGGPYLGFMA
                     TRRQYVRQLPGRIVGETTDGNGKRGFVLTLQTREQHIRRDKATSNICSNEALCALAAT
                     VYLTLLGKEGLKETAQLCADKASYAYQRLTAIPGVKPRFHGKWVFNEFVFDLPCDAAD
                     VIGKLIEKGFAAGFPLSRYYKGMDNSILIAVTEKRTKQQIGMLAEELEAIL"
     gene            5218..6666
                     /gene="gcvPB"
                     /locus_tag="PLE88_11315"
     CDS             5218..6666
                     /gene="gcvPB"
                     /locus_tag="PLE88_11315"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOM77095.1"
                     /translation="MKLVFEKSVKGRRGLRVAQSDVPTSIHLRKDLLRDKDAALPELS
                     ELDVVRHFTELSRRNFGVDTNFYPLGSCTMKYNPKVCERIAAYPGFAHLHPLLPQLRG
                     GGMLTQGALQVLYDMDILLREICGMAAFTMQPMAGAHGELTGIMIMAAYHRDRGNKKS
                     VVLIPDSAHGTNPASAAIAGYTVKSVPSDENGWMSIQALKEMLTDDVAGLMLTNPNTL
                     GLFDPNICKICDLIHKVDGLSYYDGANLNAIVGKVRPGDMGFDIVHLNLHKTFATPHG
                     GGGPGAGPVGVVEKLVDYLPISTVIKREDGTFALDYNKPKSIGYIAPFYGNFGVILRA
                     YVYILMAGKEGLERVAENAVLNANYIKEKLRPYYDIEHPQLCKHECVLSATRQAKNGV
                     HAIDIAKGLIDAGFHPPTMYFPLIVKEALMIEPTECESKETLDAFIEAMIAIAEKAQT
                     NPQELKDAPKTTPIGRLDETKAARQMHIAWLA"
     gene            complement(6673..>6878)
                     /locus_tag="PLE88_11320"
     CDS             complement(6673..>6878)
                     /locus_tag="PLE88_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77096.1"
                     /translation="KYPRIERAAKKIPLGIRSALVKVLFRRKIKQKFRLTAEQEQKVR
                     RALQEDMNRLRDTYGIDVSKWGF"
BASE COUNT         1813 a         1606 c         1737 g         1722 t
ORIGIN      
        1 tcaatgtcgg aaatcctaac acacgaaatg gccctaaccc agggggacag tcaccagacc
       61 tctgcataga gattttcttt cgaacgaaaa tagtagttta ccgaggcaat attggaatgg
      121 gcttcattgc agatatcatg aatagtagta ttatgaaagc ctttttctgc gaatactttg
      181 gtggcggctt ctaacagtaa tgttcgacgt tccaaaccgt ctttacgctt tgacatctgc
      241 ggaattttcc ctaaaaaaat gctaattagg cagtatttta aataattatt tcaaatggtc
      301 aattgaaaaa ttagaatatt tgataaaatg cggcggatca aggaagattg cccaggactg
      361 gatttgaacc agcacgccct aaacgggcat caggccctca acctgacgcg tctgccaatt
      421 ccgccacctg ggctttggat ataagattct accgaaaacc tgcgtttagg caagttattt
      481 ctgaaagcaa aataaaattt gagtagattt gacaaaaacc aaattatatt tgattattta
      541 cagggaatat ataataatta ttagtatgga acgaactaag ctctctactc aatgtccctg
      601 ttgcgggctg acaatgaagg tctccgcaat gagttgcccc gcctgcggcg tgaaggtcga
      661 aggaccattt gacgacttaa ttttaacccg cctttcagcc gaagaccagc ggtttttact
      721 ggattatatg ctgtccggct tcagtatcaa agcactcgaa gaaaacagcg ctttaggtta
      781 tgcggctatc cgcacccggc tggataaact cattgccaat actaaggccc tgatgcaatc
      841 agaagaacag aaaaaggcca ttttggataa actccgcaca ggggatataa acgtggccga
      901 agccaaaaaa cgattggaaa aactttaagg atgttaaccc atagaaccaa ccagtgaaca
      961 ggaactcaaa caattattag aagaaggcaa aattacggaa gaggactacc gccagctttt
     1021 agaggccatc catcaaaagg aaaagccgca ggagataacc gaaaaaccca tgcagaccag
     1081 accccgcacc ggatttggta aagcagcact gattttgttt atattgctgc cgcttgttcc
     1141 gattgccttt aatattattg taggcatgtc gggaagtgct acatgggcaa gattaagcat
     1201 ggtactggca ccatttatcc ttcttgggct actatgcggc ttattggcct ttattttcgg
     1261 catcgtcggc tggaagtcgc ctgccggcaa ggttgccgcg atcggggtac catgtctggg
     1321 cattttatgc atccccgggt tgtttctttt aatgttcatt cctgttaccc atgccgttaa
     1381 aatatcacat gcagaacaat ctaaagtgca ggatttaatc attcatcagg cctatccgct
     1441 ggattccctg gaaggattaa taacaaaagg tgaggcagag attgatgagg cggttttttc
     1501 aacggcaatg ggtctttgaa aatagaaagc gattcgccgg agaaacgaac gatccgattg
     1561 tttgaaaccg ggctgatgaa cattgaaaac cggatgctga tttatcaggc aaaactcaaa
     1621 agtgatttat caatcggcaa agcgtactta gagatgtggt gcaatatccc cggcaagggc
     1681 gagtttttct cccgcgggct tgaacagccc atttccggca taaccgaatg ggcgactgtg
     1741 caaacgccat ttcgtatcga ggcggggcag atgccggcca acatcaaact caatgtcgtc
     1801 gttgaaggaa caggaacaat ctggattgat gatatcaaac tattgttatg tccgttgaat
     1861 taatgacata actcttttat cgagtgaaag ttgcggcaat ttcgaaaaaa aactttacca
     1921 tcaaattaac acaatcttgc tatttaaatt ctgtttatag caaaatcgtt caaatattct
     1981 tgacacttat tttggtgttt gctattgttt ttaccgtctg ctgcttatca aggcagacca
     2041 agcaaactgc atcttatatc ttttatttgt aaggtcttat ggatgaaaca gacaccgttt
     2101 tacagccggc atgttgctct gggagcacgg atggtggact ttgccgactg gcaaatgccg
     2161 atacagtacc agcaaggtat tgttgcagag catttgacaa cccgccgaga tgcagggctg
     2221 tttgatgtct cgcatatggg caggtttcgc ctgacaggtc ccggcgcccc aaaattcctc
     2281 cgatatgttt taacaaacga ttgtgaaaag ctgccggcag gacaagccca ctatactatt
     2341 ttggccgata ataacggagg agcgattgat gatgcttttt tataccgtcc gagtgcagat
     2401 gcctatattc ttgtcgtaaa tgcttctaat aaggataagg attggcagca tttacagatg
     2461 caattagagc atttttctaa tgtcgtgatg gaagatgtca gcaattcggc cgcaatgctt
     2521 gctcttcagg ggccgcaaag tgaagcaatc ctgcattcac ttgcaccgac agaaatgctg
     2581 ccggaatcga agcgcaatgc cgtcggcaag atccgtatag aaaatattga tttatgcatt
     2641 gcccgcactg gttataccgg cgaatcagtt tgttttgaaa tgttcattcc ggcttcagat
     2701 gcttctgcgg tgtgggacag attaatagat gcaggggcaa tgccggttgg attaggggca
     2761 agggatacgc tccgtctgga agcaggcctg ccgctgtatg gccacgaact gggaatcgga
     2821 aaagacggtc tggaaatccc tgtttttgcc atcccgcagg cggcatttgc gatttcattt
     2881 gaagatgccg cacgagtttt tatcggcaaa cccgcccttg cccagcaggc tcaagcgcgg
     2941 cagcgataca aacagagcga tttttcgaat accgctgcac tgccgaaagt tgtgcgtcaa
     3001 atccgtctgc tggataaagg tattgcccgg caaggggcag aaattttctg gcagaaccgc
     3061 ccggtcggct gggttaccag cggtaccatg gttccgtatt ggatttttcg aaacggcatt
     3121 cggacagatg aatatgccca gcgtgcaata gggctttgca tggttgaacc caatgtgccg
     3181 attggggccg acatagaggt tcttgtacgc gataaaaaac tcaaggcaaa gatcgttaaa
     3241 aagaatcttg aaagccggtt tggacagcaa acagcagctg tctttgaata agctcaagaa
     3301 gtgtaagttc ccaaacagca cagtcagaaa gcaggagaat tgaagatgtt gattgatccg
     3361 caggcaaggt atttaaaaac ccatgaatgg gccaagaagc aaaccggcac catttttacg
     3421 gcagggctga cggcctatgc cgtcgagcag ctgggcgata tcgtttatat ggagctgccc
     3481 gaggtcggca aagagcttac agcaggccag ccttttggca ttgttgaatc ggttaaaagc
     3541 gcatcagaca tgtatgcacc actgaccggc agagttgttg ctgccaacac agaaatacct
     3601 gacaatcctg atacccttaa gcaggatgcg tatgacaaag gctggctgat taagattgaa
     3661 gctgcaaaac ctgctgaatt tgatgaactg atggatgccg atgcttaccg aaggtttctt
     3721 gaaaccgaag catagcgcaa gcgggaaccg gtttggctgc aattctttac ttattaaatg
     3781 ataatacggt gagagggata tgccttatat agcgaatact gaccagcagc gagcggctat
     3841 gctggctgac atcggattga ctccggaagc tttatttgcc gatgtaccgc cgtcgctgcg
     3901 cagcagcggg ctgaatctgc ccgacggaat gagcgagcag gaagtccgcg accggctggc
     3961 ggccattgcg gctaagaatt atattcatct gacacttttt ctgggcggcg gtttttacga
     4021 ccattttata ccttctgctg tctatgccat tctttcccgc agcgaatttt acacggccta
     4081 tacaccctat cagccggaaa tttcacaagg tacgcttcag gccatctatg agtttcaatc
     4141 cctgatatgc cgtctaacgg agatggaggc cgccaatgcc tctatgtatg acggcggaac
     4201 agcgctgtac gaagcgatga tgatggcgct gcggattacc cagcggagga aggttattat
     4261 cgacgattcg gttaatccta tttaccgtgt gatgattcaa tcctatacgc agaatctgca
     4321 aattgatttg gtggagacaa acaaccacga gggcttggca aaccgcgagg cgatcctaaa
     4381 agcgctggat gaagacaccg ctgcagtgat ggtgcagaat cccaactttt tcggctgtat
     4441 cgacgatttt accgacattg ctcaagctgc tcatgccaaa ggagcactgc tgattgtttc
     4501 ctgctacccg atttctcttg gcatcttgaa aacgcccggg gcaatggggg cggatatcgc
     4561 cacaggcgaa gggcagtcgc tgggcttgcc gatgtctttc ggcgggccgt atctgggttt
     4621 tatggctacc cgccggcagt atgtccgcca actgcccggc cgcattgtag gcgaaacaac
     4681 cgatggtaac ggcaagcgcg gattcgtatt gactttgcaa acgcgcgagc agcatatccg
     4741 ccgcgacaag gccacctcca atatctgctc caatgaggca ctctgtgcgc ttgcagcaac
     4801 ggtctatctg actctcttag gcaaagaggg cttgaaggaa acagcccagc tttgtgccga
     4861 caaggccagc tacgcttacc agcgattgac agcgataccg ggtgtcaagc cgcggtttca
     4921 cggcaagtgg gtatttaatg agtttgtatt cgacctgcct tgcgatgcgg ccgatgtgat
     4981 tggcaagctg attgaaaaag gttttgctgc cggttttccg ctgagccgct actataaagg
     5041 tatggataat tctattttga ttgctgtcac agaaaaacgc accaaacagc aaatcggaat
     5101 gctggccgag gaactggaag cgattttgta gggaaagatg caattactaa ttgccgccat
     5161 atccccgggg tgaaaaaaga gtcgtaatct gcggcagtat ctggttaggg aagtaatatg
     5221 aaactggttt ttgaaaaaag cgtcaaaggt cggaggggac tgcgggttgc acaaagtgat
     5281 gtcccgacaa gcattcattt gcgaaaggat ttattgcggg ataaggatgc cgctttgccg
     5341 gaattgagcg agctggatgt ggtgcggcac tttaccgagc tgtcccggcg aaacttcggc
     5401 gtcgatacga atttttatcc cttaggttcc tgcacgatga aatacaatcc caaagtctgc
     5461 gagcgtattg cggcctatcc cggctttgcg catctgcatc ccttgctgcc gcagctccgc
     5521 ggcggcggga tgctcacgca gggagccttg caggtgcttt atgatatgga catcctcttg
     5581 cgtgaaatct gcggcatggc ggcctttaca atgcagccga tggccggcgc ccacggagaa
     5641 ttgaccggca ttatgataat ggccgcctat catcgcgaca ggggcaataa aaaatcggtt
     5701 gtgctgattc cagacagcgc ccacggcacc aatcccgcca gtgctgccat tgccggctat
     5761 acggtcaaat ctgttcccag cgatgaaaac ggttggatgt cgatccaagc ccttaaagaa
     5821 atgctgaccg acgatgtcgc cggattgatg ctgaccaacc ccaatacttt ggggctgttt
     5881 gatcccaata tatgcaaaat ctgtgatctc attcacaagg ttgacggatt gtcctattac
     5941 gacggggcca atctcaatgc tattgtggga aaagtccggc cgggtgacat gggttttgac
     6001 attgtgcatc tgaaccttca caaaacattt gccacccctc acggcggcgg cggccctgga
     6061 gccggacctg tgggcgttgt ggaaaaattg gtcgattacc tgccgatatc gaccgtcatc
     6121 aaacgtgaag acggcacatt cgcactggac tacaacaagc ccaagtccat cgggtatatt
     6181 gcccccttct acggcaattt cggcgtcatt ctgcgggcgt atgtttatat cctgatggcg
     6241 ggcaaagagg gtctggagcg tgtcgctgaa aacgccgttc tcaacgccaa ttatatcaaa
     6301 gaaaagctcc gcccgtatta cgatatagag catccgcagc tttgcaagca tgaatgcgtc
     6361 ctgagcgcca cccggcaggc caaaaacgga gtccatgcca tcgatatcgc caaaggcctg
     6421 attgatgccg gctttcatcc gccgacaatg tatttccccc tgattgttaa agaagccctg
     6481 atgattgagc cgactgaatg cgaaagcaag gaaaccctcg acgcttttat tgaagcgatg
     6541 attgcgattg ctgaaaaggc ccaaacaaat cctcaggagc ttaaagacgc tcccaagacg
     6601 actcctatcg gccgattgga tgaaaccaag gccgctcgtc agatgcatat tgcttggctg
     6661 gcttagcggt tcttaaaatc cccatttgct tacatctata ccgtaagtgt cgcgcagccg
     6721 gttcatatcc tcttggaggg cgcgccggac tttttgttcc tgctcggctg tcagccggaa
     6781 cttttgttta atcttccggc gaaacaggac ttttaccaat gcgctgcgga tgcccagagg
     6841 aatcttttta gccgcccgct caatgcgcgg gtatttta
//
LOCUS       DAOTBO010000123         6859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9412_length_6859_cov_5.219871, whole genome shotgun sequence.
ACCESSION   DAOTBO010000123 DAOTBO010000000
VERSION     DAOTBO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9412_length_6859_cov_5.219871"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..85)
                     /locus_tag="PLE88_11325"
     tRNA            complement(3..85)
                     /locus_tag="PLE88_11325"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Tyr,seq:gta)
     gene            complement(225..1613)
                     /locus_tag="PLE88_11330"
     CDS             complement(225..1613)
                     /locus_tag="PLE88_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOM77097.1"
                     /translation="MDRKILLSEKDIPRQWYNLAADLPTPLLPPVTPDGKPITPDMLA
                     PVFPMNLIEQEVSTQRWIDIPEEILDILYRWRPAPMRRAVYLERHLKTPAKIYYKDES
                     TSPPGSHKPNTAVPQAWYNKQFGIQRLTTETGAGQWGSALAFACKLIGLECKVFMVRI
                     SFDQKPFRKLMMQVWGANCVASPSSETNAGRAILKETPDTPGSLGIAISEAIEQAVSD
                     PTGKTRYSLGSVLNHVMLHQTIIGLEAKAQLKSIGEKMPDVVIGCVGGGSNFAGLAFP
                     FTQDKINGAKIDIIPVEPTACPTLTRAPFAYDHGDTACMTPLLPMHSLGHNFVPPPIH
                     AGGLRYHGMAPLVCQCVLEGLHSPRAIDQVECYEAALTWARTEGFICAPETSHAIAAV
                     IQEAKKAKEEGKEKVILFNYSGHGLMDLSGYDAYMSGKLKPYALPDSELEKTEACLKK
                     HPAAPIRKSGKW"
     gene            complement(1588..1908)
                     /locus_tag="PLE88_11335"
     CDS             complement(1588..1908)
                     /locus_tag="PLE88_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOM77098.1"
                     /translation="MSRNKREPEKSCIDDLCSVLCDIRQPEQMRIFLDEILTPAERKD
                     LSLRWQLMKMLQQGFPQRQIAAQLGVSLCKITRGAKILKNNQSVSKQYLIAGDKNGPK
                     DSTK"
     gene            2241..5468
                     /gene="carB"
                     /locus_tag="PLE88_11340"
     CDS             2241..5468
                     /gene="carB"
                     /locus_tag="PLE88_11340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOM77099.1"
                     /translation="MPKRKDIKKILIIGSGPIVIGQACEFDYSGAQACKVLRKEGYKV
                     VLVNSNPATIMTDPEMADRTYIEPITPEAVEQIIAKERPDSLLPTLGGQTGLNTAVAL
                     AEQGVLKKYKVRLLGANLQSIKRAEERDRFKQIIVDIGLEVPKSGYAHTWKEAQEILE
                     QVGFPAIIRPSYTLGGMGGNVAYNKEEYERFVHWGMQLSPKHQVLIEESVVGWKEYEL
                     EVMRDRKDNVVIVCSIENLDPMGIHTGDSITVAPAQTLTDKEYQMMRDAAIKIIRAIG
                     VETGGSNIQFAVNPKNGKLYVIEMNPRVSRSSALASKATGFPIAKIASLLAVGYTLDE
                     IPNDITEKTPACFEPTIDYVVTKWPRFTFEKFRQTPAELTIQMKSVGEAMAIGRTFKE
                     SLHKAIQSLEIDRYSLDGQHIVQGLDKYQLKDKLRSNCWDKLWYIAEAYRRKFTTEQI
                     YELTHIDPWFLENIRDIVHLEKTIQSVRLTKMTPALLRQIKEYGFSDRYVAGLLGKDE
                     WTFRRLRQSKGVCAVYKRVDTCGAEFEADTPYLYSTYEEECEAHPGTRRKVMILGGGP
                     NRIGQGIEFDYCCVHAALALREIGIESIMVNCNPETVSTDYDTSDRLYFEPLTFEHVM
                     NIVETEKPDGVIVQFGGQTPLKLAGALWKAGVPIIGTSPDNIARAEDRKLFNKLVAQL
                     KLRQPASGTARSYEETLEIAKRLGYPLLVRPSFVLGGRAMDIVYDEKSLRECVREAIE
                     VSGEHPILIDQFLDDATELDVDAICDGKTVVIGGIMEHIEEAGIHSGDSACSLPPMSL
                     APSIISQIKRQTKQLALALNVRGLINIQFAVKDNAVYILEVNPRASRTIPFVSKAIGV
                     PLAKLAAKVMAGMTLQQLGFTREVVRPHYSVKEAVFPFLKFPGADVILGPEMLSTGEV
                     MGLSSDFGMAYAKSQIAAGTNLPMRGTVFISVRDQDKPRAVEVARKLHALGFKILATR
                     GTCITLIEHNIPSEFVLKVVEGRPNIVDMMIDGKVDLIINTTVGEQTIRDSYSIRRTA
                     LDRQIPYATTIRGAAAMAQAIETLQKRRMQVKPIQLYYKNSNPKP"
     gene            5481..6590
                     /gene="carA"
                     /locus_tag="PLE88_11345"
     CDS             5481..6590
                     /gene="carA"
                     /locus_tag="PLE88_11345"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOM77100.1"
                     /translation="MNAILLLEDGTLFIGKSFGAEGIRCGEVVFNTAMSGYQEILTDP
                     SYHEQIITMTYPMIGNYGTNPLDWESRRVFCAGLIVKECCPYPSNFRSRDTLETFLKE
                     NGIVGLEGIDTRKLVRHIRQQGAMKGIIAAGRRPVAELKKQLDIYPGLVGRDIVSAVS
                     VKKEYRWDKGIVDVLTQQELLPKAKYSVVAYDFGIKHNILRLLRSAGCRVEVVPAQTS
                     AAAVLAKKPDGVFLSNGPGDPAAVTYAIDNVRQLLGKVPIFGICLGHQLLALAMGGRT
                     YKLKFGHRGANHPVKNLLTGKVEITSQNHGFCVDLDSLKGLDVEQTHLNLNDSTSEGI
                     RSKKLRAFSVQYHPEASPGPHDSQYLFQQFIDWMK"
BASE COUNT         1651 a         1862 c         1873 g         1473 t
ORIGIN      
        1 aatgggccga ggtggattcg aaccaccgta ggcgttagcc aatggattta cagtccatcc
       61 cctttagccg ctcgggcatc gacccttaag aactctaatt taatagcaga aatcagatat
      121 ttcaacaaaa aaactctttt acccgcaaaa aacaggcata attgtcataa aaaaacggca
      181 gcccgcagat ggactgccgt tttggatgct ttctcaagac cggcttacca ttttccgctc
      241 ttgcgaatcg gcgcagccgg atgcttttta agacaggcct cggtcttttc cagctccgaa
      301 tccggcagag catagggctt cagcttgccg gacatatagg catcgtaccc gctcaaatcc
      361 atcaggccgt gaccgctgta attgaacagg atgacttttt ctttgccttc ttctttggct
      421 tttttggctt cctgaataac ggccgcaatg gcatggctgg tctcgggggc gcagataaag
      481 ccctcggtgc gtgcccatgt cagggctgcc tcgtagcatt ccacttggtc gatggcacgg
      541 gggctgtgca ggccttccag gacgcattgg cagaccagcg gtgccattcc atggtagcgc
      601 aggccgccgg cgtgaatggg cggcggaaca aaattgtgtc ccaaactgtg catcggcagc
      661 aagggcgtca tgcaggcggt atcgccgtgg tcataagcaa acggcgcacg ggtcagggtc
      721 ggacaggccg tgggctccac agggataatg tcaatcttgg cgccgttgat tttgtcctgc
      781 gtaaacggaa atgccagtcc ggcaaagttg cttccgccgc cgacgcagcc gatgaccaca
      841 tcgggcattt tttcgccgat gctcttgagc tgggctttgg cttccagacc gataatggtc
      901 tgatggagca tgacatggtt gagcacgctg cccagcgaat agcgggtctt gccggtcgga
      961 tccgagacag cctgctcgat ggcctcggaa atggcaatgc ccagactgcc gggcgtatcg
     1021 ggcgtttctt taaggattgc cctgccggcg ttggtctcgc tgctggggct ggccacgcag
     1081 ttggctcccc agacctgcat catcagcttg cggaagggtt tctggtcaaa actgatgcgg
     1141 accataaaca ccttgcattc cagaccaatc agcttgcagg caaaggccag agccgaaccc
     1201 cactggccgg cgccggtctc ggtggtcagc cgctgaatgc cgaattgctt gttataccat
     1261 gcctgcggca cggcggtatt gggcttgtgg ctgccggggg gactggtgct ttcatcctta
     1321 tagtagattt tcgcaggagt tttcaaatgc cgctccagat agaccgctct tcgcatcggg
     1381 gccggacgcc agcggtacag aatatccaaa atctcttccg gaatgtcaat ccaccgctgc
     1441 gtgctgactt cctgctcaat cagattcatc gggaatacag gcgccagcat atccggagta
     1501 atgggttttc catccggcgt tacaggcggc agcagcggcg tcggcagatc cgcagccaga
     1561 ttataccact ggcggggaat gtctttttca cttagtagaa tctttcggtc catttttgtc
     1621 tcctgcgatt aagtactgtt tgcttactga ctggttattt ttcagaattt ttgcgccgcg
     1681 ggtaatttta cacaaactga caccaagctg ggcggctatc tggcgctggg ggaagccctg
     1741 ctgaagcatc ttcattaatt gccaccgcag cgataaatcc ttgcgttcag caggcgtaag
     1801 aatctcatcc agaaaaatgc gcatctgttc gggctggcgt atatcgcaca gaactgaaca
     1861 taaatcatca atacaagact tttcaggttc ccttttgttt ctggacatag aagcatctta
     1921 aagaattttt acttaaatgt aaagagcatt tcagcgatta aaaactcttt ttagccgggc
     1981 cggttcaggt atcagataaa aatggccgcg gatttcttta gcaaaatggc cactccgctg
     2041 gcggcagttc caaaaaaaac aaaaatattc gcacaaaatt ttctaaattt tattgaattg
     2101 tataatgcaa ctatgtaaag ttgttccaat tttagactga acggccggtg tctgcgtatt
     2161 tcacaattca atacatccga aataggaccc ggatgacaat aaggagttaa cagccgcaag
     2221 ttttgaatcg gggactctga atgcctaaac ggaaagatat aaagaagata ctgataatcg
     2281 ggtcggggcc gattgtaatc ggtcaggcct gtgaatttga ctattccggt gcacaggcct
     2341 gcaaagtgct gcggaaggag gggtataagg ttgtactggt caacagcaat ccggccacga
     2401 ttatgaccga tccggaaatg gccgaccgca cctatattga accgattacg cccgaggcag
     2461 tcgaacaaat catcgccaaa gagcggccgg acagcttatt gccgacattg ggcggacaaa
     2521 caggactcaa tacagccgta gcgctggccg aacaaggggt cctgaaaaaa tacaaggtgc
     2581 ggctgctggg ggccaatctg caatccatca agcgggccga agaacgcgac cggttcaagc
     2641 agattatcgt ggatatcggt ctggaagtac ccaaaagcgg atatgcccat acatggaaag
     2701 aagcgcagga gattttggag caggtcggct ttccggcgat tatccggccg tcttatacac
     2761 tgggcgggat gggcggcaat gtggcctaca acaaggaaga atacgaacgg tttgtccact
     2821 gggggatgca gctgtcgccc aagcatcaag tactgattga ggagtcggtc gtcggctgga
     2881 aggaatatga gctggaggtc atgcgcgacc gcaaagacaa tgttgtgatt gtctgctcga
     2941 ttgaaaatct cgacccgatg ggcatccaca ccggcgacag catcactgtt gctccggccc
     3001 agacgctgac agataaagaa taccagatga tgcgggatgc agcgattaaa atcattcggg
     3061 ctatcggcgt ggaaaccggc ggctcgaaca tccagtttgc cgtcaatccc aaaaacggca
     3121 agctgtatgt catcgaaatg aacccgcgtg tctcgcgaag ctcggcactg gccagcaagg
     3181 ccaccggctt tccgattgcc aaaattgcct cgctgctggc ggtgggctat acgctggacg
     3241 aaatccccaa tgacatcaca gaaaaaacgc cagcctgctt tgagccgacc attgactatg
     3301 tcgtgaccaa atggccacgc tttacctttg aaaaattccg acagacgccg gcggaactga
     3361 cgatccagat gaaatcggtc ggcgaagcca tggccatcgg acgcaccttc aaggaatcgc
     3421 tgcataaggc catccagtcc ctcgagattg accgttattc gctggacggc caacacattg
     3481 tgcaggggct ggataaatac cagctgaagg acaagctgcg gtccaactgc tgggacaagc
     3541 tgtggtatat tgccgaggcc taccggcgga aattcaccac cgagcaaatt tatgagctga
     3601 cccatattga tccatggttt ctggagaata tccgggatat tgtccacctt gaaaagacca
     3661 tccagtcggt ccgtttgacc aagatgacgc ctgcccttct gcggcagatt aaggaatacg
     3721 gcttcagcga ccgttatgtg gccggactgc tgggcaaaga cgaatggact ttccgccgcc
     3781 tgcgccagtc caaaggcgtc tgtgcggtgt acaagcgggt ggatacctgc ggagcagaat
     3841 ttgaagccga caccccctac ctgtattcca cctatgaaga ggaatgcgaa gcccatcccg
     3901 gcacacggcg caaagtgatg attttgggcg gcggacccaa ccgcatcggg cagggcatcg
     3961 agtttgacta ctgctgcgtg catgcagcgc tggccctgcg ggaaatcggc attgagtcga
     4021 ttatggtcaa ctgcaatccc gagacggtca gcaccgatta cgacacctcc gaccggttgt
     4081 acttcgagcc gctgacattt gagcatgtga tgaacatcgt ggaaacggaa aagcccgacg
     4141 gtgtaattgt acagttcggc gggcagaccc cgctgaagct ggccggggcc ttgtggaagg
     4201 caggcgtgcc gatcatcggc accagccccg acaacatcgc gcgggccgaa gaccgcaagc
     4261 tgttcaacaa actggtagcc caactcaagt tgcgtcagcc cgccagcggc acagcccgca
     4321 gctacgagga gacgctggag attgccaagc ggctgggcta tcccctgctg gtacggccgt
     4381 cgtttgtgct gggcgggcgg gcaatggata ttgtctatga tgaaaagtct ctgcgggaat
     4441 gcgtccggga agccatcgag gtatcgggag agcatccaat cctgattgac cagtttttgg
     4501 acgatgccac cgagctggat gtcgatgcca tctgcgacgg caagaccgtc gtcatcggcg
     4561 gcattatgga gcacatcgaa gaggcaggca ttcactccgg cgacagcgcc tgcagtctgc
     4621 cgccgatgtc gctggcgccg tctattatca gccaaatcaa acggcagacc aaacagctgg
     4681 cgttggcgct gaacgtgcgg ggcctgatta atatccagtt tgccgtcaaa gacaatgccg
     4741 tttacattct ggaagtcaac ccgcgggcgt cgcggaccat tccctttgtc agcaaggcta
     4801 tcggcgtgcc gctggccaag ctggccgcca aagtaatggc gggaatgacg ctccagcagc
     4861 tgggattcac ccgcgaagtg gttcggccgc attattcggt caaggaagcg gtcttcccct
     4921 tcctgaaatt ccccggcgcc gatgtcattc tcggcccgga aatgctgtcc accggcgaag
     4981 tgatggggct cagcagcgat ttcggaatgg cctacgccaa atcgcagatt gccgctggca
     5041 ccaatctgcc gatgcggggg actgtcttta tcagcgtgcg ggatcaggac aaaccccgtg
     5101 ctgtcgaggt tgcccgaaag ctccacgcat tggggttcaa aatattggcc acccgcggta
     5161 cctgcatcac gctgattgag cacaacattc cttccgagtt tgtcctcaaa gtcgtggaag
     5221 gacggcccaa catcgtggat atgatgattg acggcaaagt ggatttgatt atcaatacca
     5281 ccgtcggcga acagaccata cgcgactcct actccatccg ccgcacggct ttggaccgtc
     5341 agattcccta tgccaccacc atccgcgggg cggccgcgat ggcccaagcc atcgaaaccc
     5401 tgcagaagcg ccggatgcag gtcaagccga ttcagttgta ttacaaaaac agcaacccca
     5461 aaccataaag gatgcagact gtgaatgcca tattgctgct ggaagacgga accctgttca
     5521 tcggcaaatc cttcggagcc gagggaatcc gctgcggaga agttgtattc aacaccgcga
     5581 tgagcggcta tcaggaaatc ctgacagatc catcctatca tgaacagatt atcaccatga
     5641 cctatccgat gataggcaat tacggcacca atccccttga ctgggaatcc cgccgggtct
     5701 tctgtgccgg attgatcgtc aaggaatgct gcccctatcc gagcaatttc cgcagccgcg
     5761 atacgctgga aaccttcctg aaagaaaacg gcatcgtagg actggaaggc atcgacaccc
     5821 gcaagctggt ccggcacatc cgccagcagg gcgccatgaa aggcattatt gccgcaggcc
     5881 ggcggcctgt tgccgaatta aaaaagcagc tggatatcta tcccggcctt gtcggccgcg
     5941 acattgtcag cgctgtgtcg gtcaaaaaag aataccgctg ggacaaaggc attgtcgatg
     6001 tcctgaccca gcaggagctg ctgcccaaag ccaagtattc ggtggtggct tatgacttcg
     6061 gcatcaagca caacatcctg cgtctgctgc ggtctgccgg ctgccgggtc gaagtggtgc
     6121 cggcacaaac cagcgctgcg gcggtgctgg ccaaaaagcc cgacggcgtc ttcctgagca
     6181 acggccccgg cgatccggcc gctgtaacct acgccatcga caatgtccgc cagctgctgg
     6241 gcaaggtgcc gattttcggg atttgtctgg gacatcagct gctggcactg gcgatgggcg
     6301 gcagaaccta caagctcaaa ttcggccatc gcggcgccaa ccatccggtt aaaaacctcc
     6361 tgaccggtaa agtcgagatt acctctcaaa atcacggttt ctgcgtcgat ttagactcac
     6421 tcaaaggact ggatgtagag cagacccatc tgaatctaaa cgacagcacc agtgaaggta
     6481 tccgctccaa aaaattgcgg gccttttccg tccagtacca tcccgaggcg tcgcccggac
     6541 cgcacgactc gcagtatctt ttccagcagt ttatcgactg gatgaaataa cagactatcg
     6601 ccggctgggt cagattaaac tgaacatccc tcgttgcact gcggccgctg aacaaccgga
     6661 ctaaacccag cggtattatc ggcaaatagc cgccggccac ttggaacagg atataccaga
     6721 tgccgttttg tgggaaatta aactatatat agaattatcg ccgatttaca gactccttta
     6781 ttcaaagacc gcatccggag ggactcgaac ccccaaccct cggttccgaa gaccgatgct
     6841 ctgtccaatt gagctacgg
//
LOCUS       DAOTBO010000124         6810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9515_length_6810_cov_4.338268, whole genome shotgun sequence.
ACCESSION   DAOTBO010000124 DAOTBO010000000
VERSION     DAOTBO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9515_length_6810_cov_4.338268"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4416
                     /locus_tag="PLE88_11350"
     CDS             <1..4416
                     /locus_tag="PLE88_11350"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM77101.1"
                     /translation="ANKAVQIMNAWASTHTAWDGNSNVFLRMGLYGYAFACAAELMRG
                     YSGWAPADFAAFQQYMRTQFYSGNSAWLAGQNNSHYWANWTLANMCSMLAIGVLCDDQ
                     AIFDEALNHFYNGVHTGGIEHSVYYVHPNGLGQWQESGRDQGHAQIGPQLIGVFCEIA
                     WNQGYDLYGYLNNRVLAGVEYISKYNTGHAVPFAAYVNSDYAIWPNVYRFVQKDVAGP
                     GGVSRPGWDLIYNHYVNRMGITAPYTAKYAEVMRPEGGGSNYGGTSGGFDGLGFTTLT
                     HSRDPIASGAVPGTLRPYVQGRQITLSWAGSAYAQSYNVKRSATSGGPYTTLGTVDGK
                     DLFYIDPGLTAGTTYYYVVSANNPGGESANSAEAAATADGQLFGTVIGSDYSYDHCGA
                     TREILFDGSLENFIDIGQSGGGWAGLDLGSGVSAVITGVKYCPRKGFANAMVGGKFQA
                     SNTADFSGGVIDLYTITTAPSEGVLTSRTVNSGSAYRYVRYIQSANNGWNTAAEVQFL
                     GTASGQSVPSVPVVEATLVNGLQIDLSLSSLSGVPGVGGYRIKRSTTPGGPYVIWGDV
                     NTGYGDTTKFSDVETEVNATYYYVVSAYNQIGESANSVEVSISTLPTGPQLMTHLKMS
                     GNVADCSGYNFQAGTVGSPEYSGGGYVGSAIDLDGVDDFVTLHSHVVDSDDITVAAWV
                     NWDGGAAWQRIFDFGNNTSQYIFLSPLSGGNTLRFAIKNGGAEQIVETTQLSTGVWVH
                     VAVTLGGNTARLYVNGVLRATNAGVTINPSDFKPRVNYIGKSQWADPLFNGRVDEFRV
                     YNYALSAGEVAALAAQAPETTPPAAPTELSATAGDGTVNLNWADNGESDFASYTVYRS
                     TTSGSGYSMIASGLTASAYIDNTVSNGLTYYYVVTAIDTSGGESADSSESSATPRGMP
                     IAHYKLEGNTLDGSGNGNNATTTGSPAYVEGRNGQAIDLDGLDDYITIPSGVVGAVEN
                     ITVAAWVNWDGGGSWQRIFDFGDSTSRYMFLTPSSGGSGTLRFAINNGKGEQITETSV
                     LTTGTWVHVAVTLSGNTATLYVGGFPVAINVASINPTGFDFKPAKNYIGKSQFGSDPL
                     FNGRIDDFRIYNYALSETQVAALAANTEPPSFTVKPINNLLAIEGTAYAGNSLAAYAD
                     DPDGMETVTFSKDDGPAWLKVALDGTLFGTPHDSDVGENTFTVRVTDDGGLYDTAAMT
                     IQVANTFSGTQGLDDLLGLASQWLAIDCLDTPACRGADLDSDFDVTVTDLAVLAHNWL
                     GNEDLQLHLKFDETSGNTAYDSSLYWRAGTLVNNPVWSAGYSGGALNYDGVDDYVQVT
                     GYKGVTGTASRTCTAWIKTTTPSSQIISWGATLTGEKWMFRVNEDGTLRAEVHGGYIY
                     GTTPVADGNWHHVAVVLTDDGSPNINEAVLYIDGLPETTLGGVAACPVNTAAAADVKV
                     GVNTPGTVFFKGLMDEVRIYDTVLTQQEVQQIAGL"
     gene            4450..>6810
                     /locus_tag="PLE88_11355"
     CDS             4450..>6810
                     /locus_tag="PLE88_11355"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM77102.1"
                     /translation="MTKALCVQFLAGVLVLAGLQAVYASAVPLPSRWGDWQVWGDMGD
                     GTYRNPVLPADYSDLDCIRVGSDYYAISSTMQFCPGMVILHSKDLVNWTIIGHAVSDT
                     SQIGPQMDWNRMNRYGRGVWAGAIRYYVGKFRVYFGTPDEGYFMTTAANPAGPWEPLH
                     CLKNESGWDDCCPFWDEDGYAYFVGTRFADNYKTYIWQMSPDGCTLIEESRTLINEGA
                     GREASKLYKINGWYYHFFSEVLNGTRVVMMQRSHSIMGPYTERRQLTAGNREWNEPNQ
                     GGLVQNESGDWYFLTHHGSGDWAGRCMSLLPVTWVDGWPIIGQTSTGEPGKMVLSGTK
                     PVQGQPVTTPQSSDDFNGSTLGPQWEWNYHPRMDKWSLTERPGCLRLKAFTPLRTDDL
                     MTAGNTLTQRSFRTSENIVIAKFDLSGMADGQKAGLCHFAKNHSALGVVQDGSVRRME
                     YRENGSLTQGPVIKQNQLWIKSSWGLDGKSRYAYSLDGDKFVPFGNEYQLSWGYYRGD
                     RIGLYCFNNKEDAGFVDVDFLNYDYASPLHTTTTETGPLTPISVDRANGIMLGSWNTQ
                     VEFDDIKIASGAEAVFSDSFDTERPDWQRNSGQWQVSNGVFRQISNATPALTTFMISE
                     PLTNYTVSVRAQKTEGTEGFLVGFGVQDIENYYWLNLGGWNNSRHQVEKTMYGQRYPI
                     GPTATGSIETGRWYDIQIEVEGRRIRCFLDGQRILDLTDAGFVHGVLSADNDKLNFGQ
                     ALIPDMAADPSISEIDGTFYLYATTDGWGQGLSTSGTPIVWTSKDFL"
BASE COUNT         1478 a         1932 c         1996 g         1404 t
ORIGIN      
        1 gcgaacaagg cggttcaaat catgaacgcc tgggcctcca cgcacacggc atgggatggc
       61 aactcgaacg tcttcctgcg gatgggcctc tatggctatg cgtttgcctg cgcggcggaa
      121 ctgatgcgcg gctacagtgg ttgggctccg gcggattttg cggcattcca gcaatacatg
      181 cgcacccagt tttactcggg caactccgcg tggctcgcgg gtcagaacaa cagccattac
      241 tgggcaaact ggaccctggc gaacatgtgt tcgatgctgg ccatcggggt gctgtgcgac
      301 gatcaggcga ttttcgacga ggccctgaac cacttctaca atggcgtcca cactggcggg
      361 atcgaacatt cggtctatta cgttcacccc aacggtctgg gccagtggca ggaaagcggc
      421 cgcgaccagg ggcacgccca gatagggccg cagttgatcg gtgtcttctg cgaaatcgcc
      481 tggaatcagg gctacgatct ctatggctac ctaaacaacc gggtcctggc gggtgttgaa
      541 tacatctcga aatacaatac cgggcacgcc gtgccgttcg ccgcctacgt gaactccgat
      601 tacgccatat ggccgaacgt ttatcgtttc gtccaaaagg acgtcgccgg cccgggtggt
      661 gtgagccggc ccggctggga tttgatttac aaccactacg tcaaccgcat gggcatcacc
      721 gcgccttata cggcgaaata cgccgaagta atgcgtcccg aaggcggcgg cagcaattac
      781 ggcggtacta gcggcggatt cgacggtctg ggtttcacca cgctgacgca cagccgcgat
      841 ccgattgcgt cgggcgccgt gccgggcacg ctgcggccgt atgtgcaggg gcgtcaaatt
      901 acgctctctt gggcgggatc ggcctatgcc cagagttaca acgtcaagcg ttccgcgaca
      961 agcggcgggc cttacaccac gctgggcacg gttgacggca aggatttgtt ctacatcgat
     1021 ccgggtctga cggcgggcac gacctattat tacgtcgttt cggccaataa tcccggcggc
     1081 gagagcgcca actccgccga agccgccgcc acggccgacg gtcagctttt tgggacggtc
     1141 atcggttcgg actattcgta tgaccattgc ggcgccaccc gggaaattct cttcgacggc
     1201 tcgcttgaaa acttcattga tatcggccag tccggcggtg gctgggccgg cctggacctg
     1261 ggatcgggcg ttagcgcagt gattacgggt gtgaagtatt gcccgcgcaa agggttcgcc
     1321 aacgccatgg ttggcggcaa attccaggcc tcgaatacgg ccgacttcag tggtggtgtg
     1381 attgatctgt acaccatcac caccgcgccg tccgaaggcg tgctcacttc ccggaccgtc
     1441 aatagcggct cggcctaccg ctacgtccgc tacatccagt ccgccaataa cggctggaac
     1501 accgcggcgg aggtgcaatt cctcgggacc gccagcggcc agagtgtccc gtcagtgccg
     1561 gtcgtggagg ccacccttgt caacgggctt cagatcgacc tctcgctgag cagccttagc
     1621 ggcgttccgg gcgtgggagg ctatcgaatc aagcgttcca ccactcccgg cggcccctac
     1681 gtcatttggg gggacgtcaa tacaggctac ggcgacacga caaagttcag cgatgtcgaa
     1741 accgaggtga acgcgaccta ttattatgtc gtcagtgcct ataatcagat cggcgaaagc
     1801 gccaattccg tcgaggtttc catctcgacg ctgccgacag gcccccaact gatgacccat
     1861 ctcaagatga gcgggaacgt cgccgactgt tcgggctata acttccaggc cggcacggtc
     1921 ggctcccccg aatattccgg tggggggtat gtcggctcgg cgatcgacct ggacggcgtg
     1981 gacgacttcg tgacgttaca ctcccacgtg gttgactccg acgacatcac ggtcgcggcc
     2041 tgggtcaact gggacggcgg cgccgcctgg cagcgtatct tcgacttcgg caacaatacc
     2101 tcgcaataca tctttctgtc tccgctttca ggcggtaaca cgctgcggtt tgccatcaaa
     2161 aacggcggcg cagaacagat cgtcgagacg actcagttgt caactggtgt ttgggtgcat
     2221 gtggcagtta cgttaggggg caatacggca cgactgtatg ttaacggtgt attgcgagcc
     2281 accaatgccg gcgtgacgat caacccgtcg gacttcaagc cgcgagtcaa ctacatcggc
     2341 aagagtcagt gggccgatcc gctgttcaac ggccgcgttg acgaattccg cgtgtacaac
     2401 tacgcgttgt cggcgggaga ggtggccgcc ctggccgccc aggcccccga gacgactccg
     2461 cccgcagccc ccacggaact gagcgcgacg gccggcgacg gaacggttaa tctcaattgg
     2521 gcggacaacg gcgaatccga tttcgcttcc tatacagtct atcgctcgac caccagcggc
     2581 agcggctaca gtatgatcgc ttcggggctg acagcaagcg cctacatcga caacaccgtc
     2641 tcgaacggcc tgacgtacta ctatgtcgtg acggctatcg atacgagcgg cggcgaatca
     2701 gccgacagca gcgagtcctc ggccactccc cgagggatgc cgatcgccca ttataaactg
     2761 gagggcaaca cgcttgacgg ttcgggcaac ggcaacaacg cgaccacgac cggctcgccg
     2821 gcgtatgtcg agggacggaa cggccaggca atcgatctcg atggcttgga cgattatatc
     2881 acgatcccgt ccggcgtggt cggcgccgtg gagaacatta ccgtcgccgc atgggttaac
     2941 tgggacggcg gcggttcatg gcagcgaatc ttcgatttcg gagacagcac gagccggtac
     3001 atgttcctca cgcccagcag cggcggcagc ggcacgttgc ggttcgcgat caacaacggc
     3061 aaaggcgagc agataaccga aacttccgta ctgacgaccg ggacatgggt tcacgtggct
     3121 gtgacgctca gcggcaacac agcgacgctc tatgtgggcg gctttccggt agcgatcaac
     3181 gtcgcctcca tcaatccgac cggcttcgac ttcaagccgg cgaagaacta catcggcaag
     3241 agccagttcg gctccgatcc gctgtttaac ggccgcattg acgacttccg tatttataac
     3301 tacgcgcttt cggagaccca agtcgccgcc ctggcggcta atacggaacc gccgtcattc
     3361 acggtcaaac caattaacaa tctgcttgcc atcgagggta ctgcatatgc gggcaattca
     3421 ctggccgctt atgccgatga cccggacggc atggaaacgg tcacgttcag caaagacgac
     3481 ggaccggcct ggctaaaggt ggcgctggat ggaacacttt tcggaactcc tcacgactcg
     3541 gatgttggtg aaaatacatt taccgttcgc gtaaccgatg acggcggctt gtacgatacc
     3601 gccgcgatga cgattcaggt ggctaatacc ttttccggta cacaaggact ggatgatctg
     3661 cttggtctgg catctcaatg gctggcgata gattgccttg ataccccggc ctgccgcgga
     3721 gctgacctag acagcgattt tgacgtaacc gttaccgacc tggccgttct ggcccataac
     3781 tggctgggca atgaagacct tcaactgcat ctgaaatttg atgaaaccag cggtaacacc
     3841 gcctatgaca gttcgctcta ttggcgagcc ggaacacttg tcaataatcc cgtctggtct
     3901 gccggttatt ccggcggtgc cttgaactat gatggggtcg atgattatgt ccaggttacc
     3961 gggtataaag gcgtcaccgg cactgccagc cggacctgca ccgcctggat aaaaacaaca
     4021 accccttcgt ctcaaatcat aagctggggg gcaaccctga cgggtgaaaa gtggatgttt
     4081 cgggtcaacg aagatggaac gctccgggcg gaagtccatg gcgggtacat ttacggaacc
     4141 actccggttg ctgacggaaa ctggcatcat gtagcggtgg tcttaacgga tgacggctcg
     4201 cccaatatca atgaagcggt tttgtacatc gatggcttgc ccgaaacgac ccttggaggc
     4261 gttgcggcct gcccggtcaa caccgccgcc gctgcggatg ttaaagtcgg cgtcaacaca
     4321 ccgggtactg tctttttcaa gggactgatg gatgaggtcc gaatctatga tacggttctg
     4381 acacagcaag aggtgcagca aatcgcaggt ctctaaaaaa ataataagcc aatggattgg
     4441 attgttcttg tgacaaaagc attgtgtgtt cagttccttg caggtgtact tgtattggcc
     4501 ggattgcagg cggtatatgc ctcggccgtg ccccttccca gccgctgggg tgactggcag
     4561 gtttggggcg acatggggga cggaacctat cgcaaccccg tgttgccggc agactacagc
     4621 gatcttgact gcatccgggt cggctcggat tattatgcga tctcctccac catgcagttt
     4681 tgcccgggga tggtcatcct gcactccaaa gatctggtta actggacaat catcggccat
     4741 gccgtctccg atacctctca gatcgggccc cagatggatt ggaatcgcat gaaccgttac
     4801 ggcaggggcg tctgggccgg ggccattcgc tattacgtcg gcaagttccg ggtgtatttc
     4861 ggcacgccgg acgaagggta ttttatgacc acggcagcaa atccagccgg cccttgggaa
     4921 ccgctgcact gtttgaaaaa cgaatccggg tgggatgatt gctgcccttt ctgggacgaa
     4981 gatggatatg cctactttgt cggcacgcgc tttgctgata actacaaaac ctatatctgg
     5041 caaatgagcc ccgacggttg cacactcatc gaggaatcgc gtacgctgat caacgaaggg
     5101 gccggccgcg aagccagcaa actctacaaa atcaatggct ggtactacca tttcttcagt
     5161 gaagtcctca atggtacgcg ggtcgtcatg atgcagcgtt cgcactccat tatggggcct
     5221 tatactgaac ggcggcagtt gactgccggc aaccgcgaat ggaatgaacc gaaccagggc
     5281 ggcttggttc aaaacgaatc gggggactgg tactttctca cccatcacgg ttccggcgac
     5341 tgggcaggcc gatgcatgag cttactgccg gtcacctggg ttgacggatg gccgattata
     5401 ggtcaaacca gcacgggtga gccgggaaaa atggtgctgt ccggcacgaa acccgtgcag
     5461 ggacagccgg tgaccacccc gcaaagcagc gacgatttca acggatcgac actcggtccg
     5521 caatgggaat ggaactatca ccctcgcatg gacaagtggt cgcttacgga gcgtcccggc
     5581 tgcctgcgcc tcaaggcatt taccccgctt cggacggatg atctcatgac ggccggaaac
     5641 accctcaccc agcgatcttt ccgtacatcc gagaacatcg tgattgcaaa gttcgatctg
     5701 tcgggtatgg cggacggcca gaaggcgggg ctgtgtcatt tcgccaaaaa ccactcggcc
     5761 ctgggcgtgg tgcaggacgg ctctgttcgc cggatggagt atcgcgaaaa cgggagtctg
     5821 acccaaggac ccgtgatcaa gcaaaaccag ctttggataa aatcgtcgtg gggcttggat
     5881 ggtaaaagcc gatatgccta cagtctcgat ggcgacaagt tcgttccttt cggtaacgag
     5941 taccagctat catggggcta ctaccggggt gaccgcatcg ggctttactg cttcaacaac
     6001 aaggaagatg cgggatttgt agatgtcgat tttctgaact atgactacgc ttcaccgctc
     6061 cacacaacga ccacggaaac aggaccgctt acgccgatat cggtcgacag ggccaacggc
     6121 attatgctgg gttcgtggaa cacgcaggtg gagtttgatg atatcaaaat cgcaagcgga
     6181 gccgaagcag tctttagcga ttcattcgat acagagcggc ccgattggca gcgtaacagc
     6241 ggacagtggc aggtttccaa cggcgttttc aggcagattt caaatgcgac gccggcgttg
     6301 acgaccttca tgatctctga accgttgacc aattacaccg tttcagttcg tgcccaaaag
     6361 accgaaggta ctgaaggatt tctcgtgggt ttcggtgtgc aggatatcga aaattactat
     6421 tggctgaatc ttggcggctg gaacaacagc cgtcaccagg tggaaaaaac catgtacgga
     6481 cagcgatacc ctatcgggcc gacggcgacc ggtagcatcg aaaccggtcg ctggtatgat
     6541 attcagatcg aggtggaagg ccgccgcatc cggtgttttc tggacgggca acgaatactt
     6601 gacctgacgg atgcgggatt tgttcatggt gtattgtcgg ccgacaatga caaactcaac
     6661 ttcggccagg cgctgattcc ggacatggca gccgatccga gcatttcaga aatcgacggc
     6721 actttttatc tgtatgccac caccgacggc tggggacagg gattgtccac ctccggcacg
     6781 ccgatcgtct ggacgtcaaa agacttcctc
//
LOCUS       DAOTBO010000125         6756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9610_length_6756_cov_5.448142, whole genome shotgun sequence.
ACCESSION   DAOTBO010000125 DAOTBO010000000
VERSION     DAOTBO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9610_length_6756_cov_5.448142"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLE88_11360"
     CDS             <1..1197
                     /locus_tag="PLE88_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77103.1"
                     /translation="GYGGGVGGMGGGGYGGGGYGGGGYGGGGYGGGGYGGSQGVGNWR
                     SMYRAYMLIYTIQQTVEPSTWYDEGGEGRIDQYSESKLIIYQSPEVHKQIKKLLEQLR
                     ADLGQQIAIEARFLLVSENFLEDIGLDVNIRQMDVGGHWGNVVIEQDSYGHVVPSSTG
                     VAGSLGGALTNPALNTTLTYEALDDLQVEFILRATQAHRNAKQLSAPKAVVLNNESAT
                     MRVNTAKRLKTGSEFNTETITNNNTTNQVYWWENTNEDIETGVQLSITPVITADKKFV
                     ILRVTAYLQDLIAQDTERAIGFSPVTGEQITDTYILPTTQLTSIQTRVTVPDRGTVLL
                     GGLTLTATRETESGAPVLSKLPVLGRLFSNRSLVDDKLMLLILVKPTIILQAEAEADA
                     VAALNK"
     gene            1431..2675
                     /locus_tag="PLE88_11365"
     CDS             1431..2675
                     /locus_tag="PLE88_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77104.1"
                     /translation="MKHCAENKGFCAFCWVGFHIRTLLRLFPVLSFLVLFFADCFFAH
                     SVCFGQSAAGSTPKQVLSVVQIRFGQIDPPVVAQSDITVRAATSNQQAGDAPLECSGM
                     VWLDGSLIITSDRHQHCLFVCPIDLTAMTIGRPQATVIIRNEQDLLDDAESITLKLRP
                     NGTARLYVMCSMSNNPAELPLPMRQHVLRAEIHDTRRLAFSNVSVISGSSIRNALNEH
                     FRAVGMDPYRTYYADFSGSNKNTYRWANIEGICFVPNSSLLLCGFRNPLLEEQALIAV
                     IDGIDEAFDTQDSGRLGVEDLFVLDLGGRGVSDIAWDVVTGGYLIAAAKSNGPWLDKN
                     QPFPPNQLDEALFWWSGHKSDKPILFAQVYDMKIEAVCRLGNSRFIAIASDEGDVSES
                     RSQRQSVLTILDFPGAQFNQKQ"
     gene            2685..4232
                     /locus_tag="PLE88_11370"
     CDS             2685..4232
                     /locus_tag="PLE88_11370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOM77105.1"
                     /translation="MQENQDKKKPGLEDESEFEASLDGGASGLGADKVPLQVPAAPQA
                     AEKPPSWAGKVLGHFKLLRLIGQGNMGIVIQALDVNLERLVALKVLRKRIISPEDRNR
                     VEQFFREARAAAKIDHPNVVKIYEINQHKGWWYIAMELLEGDSLKRIVEAAGPLSAQQ
                     ACPLIADAAAALDVAHREGIIHRDIKPANLMITRAGRCKLTDFGLVRMGDENDPFEIA
                     RKTVGTPYFIAPEIIEGKIQTQAVDIYSLGCTLYFALTGKPPFQAETMPELFQKHIHQ
                     PPPDVRMHCPKCSPVLAKLIGRMLAKEPSQRPTAADVAAVLRTESISLYSSDTSLLSS
                     TGTGILSQAIEKALDSMTEIPTPKLPPSTFLGRIKAAGWKKYLAVGVPVSLFVLLVLT
                     QIFWAFPFRFHKTPSVKRLESRFPDRRSSYGILPAQSVPQLPQQLNQPPPFSWLGKID
                     TTGLTYASAKNGRYYYSLSDPMAILIRQEDFIGYQTAEQARADGKLPAHQNPVWMDTP
                     IDSPIYP"
     gene            complement(4242..5573)
                     /locus_tag="PLE88_11375"
     CDS             complement(4242..5573)
                     /locus_tag="PLE88_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOM77106.1"
                     /translation="MFGCHIGRNFQVTVAGGSYQEGLTAVVQGCPPGMILTEQEIYGD
                     LLLRKPGADELSSPRKEPDLPIIYTGLNAADTVEGANNKNHTNGTPLTILIPNLDRHF
                     IHIKQYQDTNRTPRPGHASYASFVKYGPDDDAIGAGIFSGRYTSTIVAAGYVAKKILK
                     AFGIEVFSFIKEMAGVRCSEMDSKAIFSCTERYKKMRHDFDPFYQEIFVKGRITPDMR
                     FLEKTAVLAQVEKEIDDIRSQAPKMAVNEIIERYNVHPIVNCPDIDTANAMVAACNKI
                     TKTGDSSGGVVEVRVMGVPTGLGEPVFFKLDAELGRMLGIGAVKGVEVGAGFAVKDMT
                     GSQSNDAMHAENGKVVFDSNNAGGITGGLSTGQDIIVRLAVKPTPTIDKPQHTIDKYT
                     LKNTQLAAITRRDPTIVGRIWPVAENYTAMVILDHLLSHLGYQQMKSHFSR"
     gene            5855..>6756
                     /locus_tag="PLE88_11380"
     CDS             5855..>6756
                     /locus_tag="PLE88_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77107.1"
                     /translation="MDRKRFDIEGRQIIIHTQGGICTTASDLLHSDIFRQVVALYITV
                     CRQQQAPQLGIFKNIFPSETETDAITELLIQLADVPLEEVVKDLPNLAGLLERQNRLH
                     LHEFAEGLYDYWRSFDRFMVLHSEPGKSSFDRRPYRSFNATVETLTHIVRSTYRDICE
                     NITGDHPRIYRQVFAGCDIGLIAVAKRTRMPAAYEAVLGDVPFIRHVWIAPPMIIDPP
                     MNKRDGQFKKTDINPLDGLTLEKGHWLCYPAQVGPLVIFVYFHTNQIGLGCSLANLFE
                     LATDQQIADGPDAVYCFGVNPEHM"
BASE COUNT         1590 a         1651 c         1804 g         1711 t
ORIGIN      
        1 ggctacggcg gcggcgtggg cggcatgggc ggcggtggtt atggcggcgg tggttatggc
       61 ggcggtggct atggcggcgg tggctatggc ggcggcggct acggcggcag ccaaggggtt
      121 ggaaactggc ggtcgatgta tcgggcttat atgctgattt ataccatcca gcagactgtt
      181 gagccaagca cgtggtatga tgaaggcggc gaaggccgta ttgaccaata cagcgaaagc
      241 aagctgatta tttatcaatc tcccgaagtg cacaagcaga ttaagaaact gctggagcag
      301 ctgcgggctg acttggggca gcagattgcc attgaagccc gcttcctgct ggtcagtgaa
      361 aacttccttg aagatatcgg cttggatgtc aatattcggc agatggatgt cggcgggcat
      421 tggggcaatg tagtgattga gcaggactcg tatggccatg tagtgccgtc cagtaccggt
      481 gtggcaggca gtctgggagg tgcgttgacc aatcctgcac tgaataccac gctgacctat
      541 gaagcgctgg atgatttgca ggtggaattt atcctgcggg cgacgcaagc ccaccgcaat
      601 gcaaaacagt tgtctgctcc caaggctgtc gtgttgaaca acgaatcggc cacgatgcga
      661 gtgaataccg ccaaacggct taagacggga agcgagttta ataccgagac gattaccaac
      721 aacaatacaa ccaatcaggt ttactggtgg gaaaatacca atgaggacat tgaaaccggc
      781 gtccagcttt ccattacacc ggttatcaca gcagacaaga aatttgttat cttgcgggtt
      841 acggcctatc tgcaggacct gattgcacag gacacagaac gagctatcgg cttcagccct
      901 gtaacagggg aacaaataac cgacacctat attttgccga ctacccagct gacctctatt
      961 cagacgcgtg ttaccgtgcc ggatcgcggg accgtgcttt tgggcggtct gactttgacg
     1021 gctacccgcg aaaccgaatc gggagctccg gttcttagca aactgccggt tttgggacgg
     1081 ctgttctcta accgcagtct ggtagatgac aagctgatgc tgctgattct ggttaagccc
     1141 acgattattc tgcaggcgga ggcggaagcc gatgctgttg cggcattaaa caagtaagcc
     1201 aatttctggt gcaataaatg caggccggac ttgtctggat gaacaggcaa ggccggcctt
     1261 ctttttatgc ctgcggcata gttccaaatt atttctcaag agatgcagcg gaaaggagcg
     1321 gttatttcag gcaggccgct gcaacgcacg acagtagaga cggcagaaag aattgacttt
     1381 tctggatagt tcggatacaa tactatgcgg aaaggaatca gcagtatgct atgaagcatt
     1441 gtgctgaaaa taaagggttt tgcgcttttt gttgggttgg gttccatatt cggacactgc
     1501 tgcggttatt tcctgtcttg tcttttctgg tgctgttttt tgccgactgc ttttttgccc
     1561 actctgtctg ttttggccaa tcagccgctg gaagcactcc gaaacaggtt ttgtctgttg
     1621 ttcagattcg attcggtcaa attgatccgc cggttgtggc tcaaagcgat attacagttc
     1681 gggcggctac ttccaaccag caggccggag atgcaccgct ggaatgcagc ggcatggtgt
     1741 ggctggatgg ttcgctgatc attaccagcg acaggcatca gcattgtctt tttgtctgcc
     1801 cgattgatct gacagcgatg accatcggac gtccccaagc cacggttatt attcgcaacg
     1861 agcaggattt gctggacgat gccgaaagca ttacgctgaa attgcggccg aacggaacgg
     1921 cccgccttta tgtgatgtgt tcgatgagca ataatccggc ggaattgcca ctgccgatgc
     1981 ggcagcatgt gctgcgtgcc gaaatccacg acacccgccg ccttgctttt tccaatgtgt
     2041 cggttatcag cggcagttct attcgaaatg ccctcaatga gcattttcga gccgtcggaa
     2101 tggatccgta tcgtacttat tatgcggatt tttcaggttc caataaaaat acctatcgat
     2161 gggccaacat cgagggaata tgttttgtac ccaacagttc tctgctgctg tgcggatttc
     2221 gaaatcccct gctggaagag caggcgctga tagctgttat tgacggaata gacgaagcgt
     2281 ttgatacaca ggattccggg cggttgggtg ttgaagatct gtttgtcttg gatttgggcg
     2341 gccgcggcgt cagcgacatt gcttgggatg ttgtaacggg aggctatttg attgctgcgg
     2401 ccaaaagcaa tggtccctgg ctggataaaa atcagccgtt cccgcccaac cagctggatg
     2461 aggccctgtt ctggtggagc ggtcataaaa gtgacaagcc cattctcttt gcccaagtct
     2521 atgatatgaa gatagaagct gtctgccgcc tcggaaacag ccgtttcatt gcgattgctt
     2581 cggatgaagg cgatgtcagc gaaagccgct cgcaaaggca gagcgttctg acgatcttgg
     2641 attttcccgg agcccaattt aaccaaaaac aatgaaacaa tgtgatgcag gaaaatcagg
     2701 ataaaaaaaa gccgggtttg gaagacgagt cggagtttga agccagtctc gatgggggtg
     2761 catcgggttt aggagctgat aaggtaccgc tgcaagtgcc cgccgcaccg caggcagcag
     2821 aaaaaccgcc ttcgtgggca ggcaaggtgc tggggcattt taagctgctg cgtttgattg
     2881 ggcagggcaa tatgggaatc gtgatacagg ccttggatgt caatcttgag cgtttagtgg
     2941 cccttaaagt tctccgcaag agaatcatat cgcctgaaga ccggaatcgg gtagagcagt
     3001 ttttcaggga agcccgagcc gcggcaaaaa tcgatcaccc caatgtggtg aagatttacg
     3061 aaattaacca gcataagggc tggtggtata tcgccatgga attgctggaa ggggatagtc
     3121 tcaagcgtat tgtcgaagcg gcgggtcctt tgtcggccca gcaggcctgc cccctgatag
     3181 cggatgctgc cgccgcgctg gatgtggctc accgcgaggg gattattcac cgtgatatca
     3241 agcccgccaa tctgatgatt acccgcgccg gccgctgcaa gctgacagat tttggtctgg
     3301 tccgcatggg cgatgaaaac gatccctttg aaatagcccg caaaaccgta ggaactccgt
     3361 attttatagc ccccgaaatt atcgagggaa aaatccagac acaggccgtt gatatttaca
     3421 gtctgggctg caccctttat tttgctctga cgggcaagcc gccttttcag gcagaaacga
     3481 tgccggaact tttccaaaag cacatccatc agccgccgcc tgatgtccgg atgcactgtc
     3541 ccaaatgttc accggtgttg gctaagctga ttggacgtat gcttgctaaa gagccttccc
     3601 agcgtcccac tgcggcggat gtggctgctg ttctgcgaac ggagtctatt tccctgtatt
     3661 catccgatac atcactgttg tcgtctaccg gtacggggat tttaagtcag gccatcgaaa
     3721 aagcccttga ttcgatgaca gaaataccga ctcctaagct cccgccgtcg acgtttttag
     3781 gacgcataaa ggcggcgggc tggaaaaaat accttgccgt cggtgtacct gttagtttgt
     3841 ttgtgctgct tgtgctgacg cagattttct gggcatttcc atttcgcttt cacaaaactc
     3901 cttctgtaaa gcggctggaa tcaaggtttc cagaccgccg ctcctcttac ggcattctgc
     3961 cggctcagtc tgttccgcag cttccgcaac agctcaatca gcctcctccc ttcagctggc
     4021 ttgggaaaat agatacaaca ggcctgactt atgcgtcggc caagaacggc cgttattatt
     4081 attcattgtc cgaccccatg gcgattctta ttcgtcaaga ggacttcatc ggataccaaa
     4141 cagccgaaca agcccgcgcc gacggcaagc ttcctgcgca ccaaaatccg gtatggatgg
     4201 ataccccgat tgactcaccg atatatccgt agcaggatat tctatcgtga aaaatgcgac
     4261 ttcatctgct gatagcccag atgcgacagc agatgatcca gaataaccat tgccgtatag
     4321 ttttcagcaa ccggccagat gcgtcctacg atggtcggat cccggcgggt gatggccgcc
     4381 agttgggtgt ttttcagggt gtatttgtcg attgtatgct gcggcttatc gatggttggg
     4441 gtcggcttta cggccagacg aacaataata tcctgaccgg tcgaaagccc gccggtgatc
     4501 ccgccggcat tgttggagtc aaacacaacc tttccatttt ccgcgtgcat tgcatcattg
     4561 gattgacttc cggtcatatc tttgaccgca aatccagcgc ctacctctac gcctttaaca
     4621 gcgccaatgc ccagcattcg tcctaactct gcatccaatt tgaaaaagac cggctctccc
     4681 aatcctgtcg gcactcccat aacgcgaact tcaacaacgc cgccggaaga atcacctgtc
     4741 ttggtgattt tattgcacgc agccaccatt gcattggccg tgtcaatatc tgggcaattt
     4801 acaatcggat gcacattata gcgttcaata atttcattaa ccgccatttt aggagcttgc
     4861 gagcggatat cgtctatctc tttttccacc tgtgccaaaa cagctgtttt ctcaagaaaa
     4921 cgcatgtctg gtgtaatcct gcccttgacg aagatttcct gataaaacgg gtcgaaatcg
     4981 tgacgcattt tcttgtaccg ctcggtgcag gaaaagatgg ctttcgaatc catttctgaa
     5041 cagcgcacac cggccatttc tttgatgaag gaaaacacct caatcccgaa ggccttgagt
     5101 atctttttgg ccacataacc ggctgcgaca atagtggatg tgtagcgtcc gctgaaaatg
     5161 cccgctccga ttgcatcgtc atccggtcca tatttgacaa atgaagcata tgacgcatgg
     5221 ccgggacgcg gtgtccgatt ggtgtcctga tactgcttaa tatgaatgaa atgacggtcc
     5281 agattgggta ttaagatcgt cagaggggtt ccattggtgt gatttttatt attggctcct
     5341 tccactgtgt cggcggcatt gagaccggtg taaataatcg gcaagtccgg ctccttacgc
     5401 gggctggaaa gctcatcagc acccggcttg cgcagcagca ggtcaccgta aatttcctgt
     5461 tctgtcaata tcattcccgg cgggcatccc tgcacgactg cagttaaacc ctcctgatag
     5521 gagccgccgg cgacagtgac ctgaaaattt cttccaatat ggcagccaaa cataatcgac
     5581 ctcctgcgtc ttttaacgct tattttttcg caaaaatact atccagatga ggtgctgttt
     5641 gcaagttttt tttatttatt ttaaaatttt gttgattgtt gtgccgcctt ggctaaaatc
     5701 acgctccgcg ctgggacgga gttatctatg atgaaagatg taaatcgggt tatcagggga
     5761 tgttgaactc caaacaggaa ctttggttgg gtatcatccg gatgccgggg ttgctatata
     5821 aagtgatata gcacagagaa aaggaaggcc gtcaatggat cgcaaacgat ttgatataga
     5881 agggcgtcag attatcattc atacgcaggg aggaatctgc acaacagcaa gcgacttgct
     5941 tcacagcgac atttttcgcc aagttgttgc cctttatatc acggtgtgca gacaacagca
     6001 ggccccccag ctgggcatat tcaaaaatat ttttccttcc gagacagaaa ccgatgccat
     6061 taccgaattg ctgattcagc ttgcggatgt gccgctggaa gaagtggtta aggatttgcc
     6121 gaatctggca ggccttctgg agcgacagaa ccgtctgcat cttcatgaat ttgcagaggg
     6181 gctttatgat tactggcgtt cgtttgaccg gtttatggtg cttcacagcg agccgggcaa
     6241 aagcagtttt gaccgccggc cttaccgttc gtttaatgcg accgttgaga cactgaccca
     6301 tatagtgcgc agcacctatc gggatatctg tgaaaacatc accggggacc atccgcgcat
     6361 ctatcgtcag gtgtttgccg gctgcgatat tggtttgatt gctgttgcca aacgcacgcg
     6421 gatgccggcg gcttatgagg cggtattggg agatgtgccg tttattcggc atgtatggat
     6481 tgcccctccg atgattattg atcccccgat gaacaagcgc gacggccagt tcaaaaaaac
     6541 cgacatcaat ccgctggacg ggctgactct tgaaaaggga cattggcttt gctatccggc
     6601 tcaagtaggt ccgctggtta tttttgtgta tttccacacc aatcaaatcg gtctgggatg
     6661 cagcttggcg aacctgttcg aactggcaac cgaccagcag atagccgacg gtcccgatgc
     6721 tgtttattgt ttcggagtga atcccgaaca tatgca
//
LOCUS       DAOTBO010000126         6627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9830_length_6627_cov_5.631771, whole genome shotgun sequence.
ACCESSION   DAOTBO010000126 DAOTBO010000000
VERSION     DAOTBO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_6627_cov_5.631771"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="purD"
                     /locus_tag="PLE88_11385"
     CDS             complement(<1..1163)
                     /gene="purD"
                     /locus_tag="PLE88_11385"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOM77108.1"
                     /translation="MKVLLVGSGGREHAIAWKLSQSKRLKKLYIAPGNPGTAQFGENI
                     DIADTNIEELVKFARGADIGLAVIGPEDPLAMGAVDAFEAAGIKAFGPSRAAAQLEAD
                     KHFAKEIMRASSIPTAESRSFTSFEDAKAYIASRDEPVVVKAAGLAKGKGVFVCDDPA
                     EGILAAEKIMKDRIFGDAGNTVVVEDKLLGQEASILAFVDGRNIYVMESAQDHKPIGD
                     GDTGPNTGGMGAYSPAPIVTDALMDQIIREILVPTIDGMNRNETPYKGVLYAGLMLTQ
                     GGPRVLEFNCRFGDPETQPILMRLKSDLLEVMLAVCDGKLDAVTLQWDERPAVCVVMS
                     SGGYPGGYEKGKVITGLEEAAKLKDVFVFHAGTARKDGAVVTSGGRVLGVTALG"
     gene            1323..1829
                     /locus_tag="PLE88_11390"
     CDS             1323..1829
                     /locus_tag="PLE88_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM77109.1"
                     /translation="MYRLNIQSAMRKQNTNRQNRTRGFTLVELMIITAILGILAAIVF
                     PEFQGHIQRAKETQAKANLRLLREAIERYAADHNGIPPGYLNNDPSATVSASEFYRQL
                     VVKGKYLNEMPKNPFNGYNGMFICANIVPFPAEADGTSGWIYKPLTKEIRLNWPDTDS
                     EGKLFYSY"
     gene            1905..4121
                     /locus_tag="PLE88_11395"
     CDS             1905..4121
                     /locus_tag="PLE88_11395"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOM77110.1"
                     /translation="MEKPKGFFAILIPAVTVFISSACIMIIELVASRLIARHLGSSLY
                     TWTAVIGVVLAGITIGNYTGGRIADRYPSQKALSFQFCLASAACVIIIILNNVVGDWI
                     WLWKLSWPMRVFLHVTLVFLLPSVLLGTISPVVAKMALDRGLPAGRTVGDIYAFGAAG
                     SIAGTFIAGFYLIAAMGTVAIIWTVAFILALMAALYRLKAWPAWLWLLLLAGFFFLGT
                     SKASAAENLGTSFLLREKIDPAVLYTDDTEYCYIAVKQISQTPDRREFIQDKLKHSEI
                     NMDNPDDLLYFYTHIYASITHGLRPQPNPLAVMVIGGGGYVYPRYIEKHWPGSQIDVV
                     EIDPGVTEAAYAAFGLPRSTTIRTHTMDARNFVDGLLLLEKNGQSVPKYDFIYEDAIN
                     DYSVPFQLVTHEFNKKIVQLLKPNGVYLVNLIEVYNSGLFLGSMIQTLEKTFPYVYVL
                     TEYAPHSIRNTFVLAASFEPLDLQAIIGRYTRGSDIWYLSEEEISGLKQRAYFGLITD
                     DYAPVENMLVPVVQRTSSDFLAVKYKNQANDLKKLGKYDESIQKYLDMIRVEPTLSIL
                     GYNEIGIMLAQQNKPDEAASYFLKAIDYNQKADVPINLASVHLNLALLLREMGKTAEA
                     AAHFQTAADGYKKELAKNPKSLKDTMLVATTLKEMGNLSEAIEYYQKAVDLNPYDTQQ
                     HITLAQAYEAVNDYDKAVAAIQKAIGFFDYINESNRAAPLKEYLKYLNFQKAQQKP"
     gene            complement(4214..4783)
                     /locus_tag="PLE88_11400"
     CDS             complement(4214..4783)
                     /locus_tag="PLE88_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77111.1"
                     /translation="MKITTAVFLIVGFMFTLSGCGQPHKGVKIIVEGGGKFPAELAGK
                     WVCPEQGWTIELDKNGYIPFMIIPMGRTEMHPGKETSFEIPKYQGKAIYRPGLWTVSW
                     SPATRELGITVELAYFYHDVGNHAVEGSTTDYLIGKVSEDGKTWKPDLQTMGFVDALL
                     FEGWTLKERRNLFDDKEPVWRGEVVFQKE"
     gene            complement(5006..5491)
                     /locus_tag="PLE88_11405"
     CDS             complement(5006..5491)
                     /locus_tag="PLE88_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77112.1"
                     /translation="MMKKLMVLALVMGVAMLSNAALVVTAPDQVNVGEAFQIIIGGDE
                     PYSFAGGLYGDGVTAVTGWSKVQGNVTGASFEAGLGGWEFLVDEVGATGSKPVPAGEW
                     IKFDVLAPSVPGMLTFDLYDYNVDLNVPVKSFAVTVAPIIPEPATMLLLGLGGLLLRR
                     K"
     gene            complement(5582..6442)
                     /locus_tag="PLE88_11410"
     CDS             complement(5582..6442)
                     /locus_tag="PLE88_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77113.1"
                     /translation="MKKILASLLVLALCAPAMAVQFGLIGNTDGTATITIDTEGAVVR
                     GVAIKVTAAGGLTDVNGATVNAAFNTYIDYAHDAGAAYTEVGQGHPFAADGAAGVAVN
                     GATTFVISMGVLDQGGNQAGFNGAGDLITFPVGDGEVCIAADTLRGPDSGVVGDAALT
                     SNIASVACVTVSSGPVDCIPSTNPSYAQWVAVGKPDSWCNPRQCHGDANGAQEQIGKV
                     MRWVGYADLNILVSGFNKVYTGTESWIAADFSRTEEQIGKVMRRVGYADLNILVSYFN
                     QGVVPDTCND"
BASE COUNT         1660 a         1901 c         1461 g         1605 t
ORIGIN      
        1 cccagcgccg tcacacccag cacccggccg ccgctggtta caacagcgcc gtcttttctg
       61 gccgttcccg catggaaaac aaacacatcc ttcaatttgg ccgcctcttc cagtcctgta
      121 atcaccttgc ccttttcata gccgcccggg taaccgccgg aactcatcac cacacacacc
      181 gccggccgct catcccattg cagcgttacg gcatccagtt tgccgtcgca aaccgccagc
      241 atcacttcca gcaggtcgct cttgagccgc atcagaatcg gctgggtttc cgggtcgccg
      301 aagcggcagt taaattccag cacgcgcggc ccgccctgcg tcagcatcag tcccgcatac
      361 agcaccccct tatacggcgt ctcgttgcgg ttcatcccat cgatcgtcgg cacaaggatc
      421 tcccgaataa tctggtccat cagggcatcc gtaacgatgg gagccgggct gtaggcgccc
      481 atcccgccgg tattcgggcc tgtgtcccca tcgcctatcg gcttatggtc ttgggcgctt
      541 tccatcacat aaatgttccg gccatccaca aacgccagaa tggatgcctc ctgccccagc
      601 agtttatcct caacaacaac tgtattgccg gcatccccaa aaatgcggtc tttcataatc
      661 ttttccgctg ccagaatccc ttcagccggg tcatcgcaga caaatacccc ttttcctttg
      721 gccagtccgg cggccttgac cacaaccggc tcatcccggc tggcaatata tgccttggca
      781 tcttcaaacg aggtaaacga acggctttca gccgtcggga tgctgctggc ccgcataatc
      841 tctttggcaa aatgcttatc cgcctcaagc tgagcggccg ccctgctggg cccaaaggcc
      901 ttgatgccgg ccgcctcgaa ggcatccacc gcacccatcg ccaaggggtc ttccggccca
      961 attacggcta aaccgatatc agcaccgcgg gcaaatttga ctaattcttc aatatttgtg
     1021 tccgctatgt ctatattctc gccaaactgg gctgtgcccg gattgccggg ggcaatatag
     1081 agctttttaa gcctcttgga ctgggataat ttccaagcaa tagcatgttc ccgaccgccg
     1141 ctgccgacca ataaaacctt cataaaaact cctctaataa tgtcaaatcc gtaccgaatg
     1201 aataataata tctttttttc acttttacca acgaaaatca gctaattgca ggtaatcatc
     1261 cggccaaaac aagccgaaaa agaaaagcgg gagatagtct atctgactat actggatgcg
     1321 ttatgtacag attaaatata caatcagcaa tgcgcaagca aaatacaaac aggcaaaacc
     1381 ggacgcgggg ctttacgctg gtcgagctga tgattatcac ggccatactg ggcatattgg
     1441 cggctattgt ttttcccgag tttcagggcc atatccaaag ggccaaagaa acacaggcaa
     1501 aggccaacct ccgcctgctc cgagaagcca tcgaacgcta cgccgccgac cataacggaa
     1561 tcccaccggg gtatctcaac aatgacccct cagcaacggt cagtgccagt gaattttaca
     1621 ggcagctcgt tgtaaaagga aaatatctca acgaaatgcc caaaaatcca tttaacggct
     1681 acaacggtat gtttatttgc gcaaacatcg ttcctttccc cgccgaagct gacggtactt
     1741 ccggttggat ttataagcct ctgacaaaag agattcgact taattggcca gatacagatt
     1801 cagaggggaa actcttttac agctactgaa ccttgccgat gccgccctgc tctggtatga
     1861 tagacagcgg atctgataac tcaataaatt aggacataat tattatggaa aagccaaaag
     1921 gattttttgc gattcttatt cctgccgtaa cagtatttat ctccagcgcc tgcataatga
     1981 ttattgagct ggtcgccagc cggctcattg cccgtcatct cggctcatct ctgtacactt
     2041 ggacagccgt cataggcgtt gtgcttgccg gcattactat cggcaattac accggcggtc
     2101 gaatcgctga ccggtatcca tctcagaaag ccctttcctt tcaattctgt ctggcatctg
     2161 cggcctgtgt gattattatt attttgaaca atgtcgtcgg cgattggata tggctgtgga
     2221 agctgagctg gccgatgcgg gtttttctcc atgtgaccct tgtttttctg ctgccctctg
     2281 tcctgctggg aaccatcagc ccggtggtgg cgaaaatggc cctcgatcga ggactgccgg
     2341 ccggccgcac ggtcggcgat atttatgcct tcggcgctgc cggcagcatc gctggaacct
     2401 tcattgccgg cttttacctg attgccgcaa tgggaaccgt cgctatcatc tggacggttg
     2461 cgtttatctt ggcgctgatg gccgccctct accggctcaa ggcatggcct gcctggctgt
     2521 ggctgctcct tttggccggc tttttttttc tcggcacttc taaagcatcc gccgctgaga
     2581 atttaggcac ttcctttctg ctgcgcgaaa aaattgaccc tgcagtcctt tacaccgatg
     2641 acacagagta ttgctacatt gccgttaagc aaatatccca aacaccagac cgccgcgaat
     2701 ttattcagga taagctcaag cacagcgaaa tcaatatgga caatcctgac gatctgctgt
     2761 acttctatac ccacatatac gcaagcataa cgcacggcct gcggccccag ccgaatcctc
     2821 ttgccgtcat ggtcatcggc ggcggcggat atgtttatcc ccgctacatc gaaaagcact
     2881 ggcccggcag tcaaatcgat gttgtcgaaa tcgatcccgg tgtgacagag gcggcctacg
     2941 ccgcttttgg tctgccgcgc agcacaacca tccgcaccca tacaatggat gcccgcaatt
     3001 ttgttgacgg ccttttgctg cttgaaaaga atggacaatc tgtccccaaa tatgatttta
     3061 tctacgaaga cgctatcaat gattactccg tcccttttca gcttgttacg cacgagttca
     3121 acaaaaaaat tgttcagctc ctcaaaccca acggcgtcta tttagtcaat ctaattgaag
     3181 tatataattc cggcttgttt ctgggcagta tgattcagac gctggaaaaa acatttcctt
     3241 atgtctatgt cctgacggaa tacgcccctc acagcatccg caacacattt gtgctggccg
     3301 cttctttcga accgcttgat ctgcaggcca ttatcggccg gtacacccgc ggctcggata
     3361 tttggtatct cagcgaagaa gaaatcagcg gcctcaagca gcgggcttat ttcgggctga
     3421 ttacagacga ttacgccccc gttgaaaata tgctggtgcc ggtggttcag cgcacaagct
     3481 ccgactttct ggctgtcaag tacaaaaatc aggctaacga ccttaagaag ctcggcaaat
     3541 atgatgaaag catacaaaaa tatctcgata tgatacgggt tgaaccaacc ctgtcgattt
     3601 tgggctacaa cgaaatcggg ataatgctgg cccagcagaa caaacccgat gaggccgctt
     3661 cctattttct caaagcaatt gactacaacc agaaagccga tgtacccatc aatctggcca
     3721 gcgttcacct gaatttagcc ctgctgctgc gtgaaatggg caaaaccgca gaagccgcag
     3781 ctcatttcca gacggccgcc gatggctaca aaaaagagct tgccaaaaat cctaaatcgc
     3841 ttaaggatac aatgctggtg gctacaaccc tcaaagagat gggaaatctg tctgaggcaa
     3901 tcgaatatta tcagaaggca gtggatctga atccctacga tacccagcag cacatcacgc
     3961 tggctcaggc ctacgaagcc gtcaatgact acgacaaggc agtcgcagcc atccaaaagg
     4021 ccatcggatt ttttgactac atcaatgaat caaatcgggc ggctccccta aaagagtatc
     4081 tcaaatacct caacttccag aaagcccagc agaagccctg acaacttaag ctccccagac
     4141 aatcagcaac gcaaaccggc ttgttcagag gctgcctgcc ggtctgccct gctgctgaca
     4201 agcaaggaag cggctattct ttttggaaca ccacttcgcc ccgccagacc ggttctttat
     4261 cgtcaaaaag gttgcgccgt tctttaagcg tccatccttc aaacagcaga gcatcaacaa
     4321 atcccatcgt ttgcaggtcc ggcttccagg tcttgccgtc ttcggatacc ttgccgatta
     4381 gataatcggt cgtgctgccc tcgacggcat gattgcctac atcgtggtaa aaataggcca
     4441 gctcaaccgt aatgcccagc tcccgggtgg ccggcgacca gctgacagtc cacaagccgg
     4501 ggcgatagat ggccttgccc tgatatttgg ggatttcaaa cgaagtttct ttgcccgggt
     4561 gcatttctgt cctgcccatc gggattatca taaatgggat atagccgttt ttatccagct
     4621 ctattgtcca gccctgttca gggcagaccc atttgccggc taattctgcg gggaatttcc
     4681 cgcccccctc cactataatt ttgacgcctt tgtgcggctg gccgcagccg gagagagtga
     4741 acataaaacc gacaataaga aatactgcag ttgtaatctt catcagaaag atacctttaa
     4801 tttacgtaaa accaagcaga accgtttaac aagcaagccc ggcggaagac ctgccgatat
     4861 tttgcttttt atactgcttt atcggtacgg caggccctat aacataaata tattgggttt
     4921 tgtacagcgt caataaaaaa agaggttctc ttttaaggga gaacctcttt cacacactta
     4981 ctttgcaaag cgtttggcta tagtgttatt tgcggcgaag cagcagaccg ccaagaccca
     5041 gcagaagcat cgttgccggt tcgggaataa ttggagctac tgtaactgcg aaactcttaa
     5101 ccgggacatt taaatctacg ttgtaatcat acagatcaaa agttaacatt cctggtacac
     5161 tgggagctaa aacatcaaat ttaatccact caccagcagg taccggcttg gatccagtgg
     5221 cgcctacttc atccaccaga aactcccatc cgcccaaacc agcttcaaag cttgcacctg
     5281 taacattacc ttgtactttc gaccagccag ttactgcagt aacaccatct ccataaaggc
     5341 cacccgcgaa actatacggt tcatctccgc ctattataat ctgaaacgct tcacccacat
     5401 ttacctgatc cggagctgtc actaccaacg ctgcatttga caacattgca acgcccataa
     5461 ccaaagccaa taccatcaac ttcttcatca tcgttctcct ttcatcatca gttttttgtt
     5521 cgaacaaact taaccgttaa cgttaacctg cactatttcg atagtgaagt gtgtgtccaa
     5581 tttagtcatt gcatgtatcc ggaactactc cttggttgaa atagctgact aaaatattta
     5641 aatcagcata accaacacgg cgcatcactt taccaatttg ctcttcagta cgcgaaaaat
     5701 ctgcagctat ccaagattct gtacccgtat aaaccttatt aaaaccactc acaagaatat
     5761 tcaagtcagc atagccaacc caccgcatta ctttaccaat ttgttcctgt gctccattgg
     5821 catcaccatg gcactgacgg gggttgcacc agctgtcggg cttgccgacg gcgacccact
     5881 gagcatagct gggattggtt gacggaatgc agtcaacggg tcctgaagaa acagtcacgc
     5941 aggccacaga agcgatattg ctggtcagag cagcatcgcc caccacgccg ctgtccgggc
     6001 cgcggagggt atcagccgca atgcagacct cgccgtcgcc aacggggaag gtgatcaggt
     6061 cgccagcacc gttgaagccg gcctggttgc cgccctgatc cagaacgccc atgctgataa
     6121 caaacgtcgt tgcgccgttg acagccacgc cggcagcgcc gtcagccgca aacggatgcc
     6181 cctgaccgac ttcggtataa gccgcgccgg cgtcatgagc atagtcaatg taagtattga
     6241 acgcagcgtt tacggtcgcg ccgttaacat cggtcagacc accggcagcc gtcaccttaa
     6301 tagcaacgcc gcgaacaaca gcaccttctg tatcaatcgt gatggtagcc gtaccatcgg
     6361 tgttgccgat cagaccaaac tgaacggcca ttgccggcgc gcacagcgct aataccagca
     6421 aacttgcgag aatctttttc attgggacac cttacctttc tctttttctt ctttgtgtct
     6481 tctcttctat catcaccctt ggggctccgc acctcaaggt tttcttaaat cccttccttt
     6541 aatcccccgc aagaggaata acagccgcct tgcggtctgc atcaccgcaa tctcgggact
     6601 tgtttacaat gctgataata caatatc
//
LOCUS       DAOTBO010000127         6552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_9988_length_6552_cov_6.557334, whole genome shotgun sequence.
ACCESSION   DAOTBO010000127 DAOTBO010000000
VERSION     DAOTBO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9988_length_6552_cov_6.557334"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLE88_11415"
     CDS             complement(<1..361)
                     /locus_tag="PLE88_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOM77114.1"
                     /translation="MFERFTDRARKVMALANQEAQRFNHEYIGTEHILLGLVKEGNGV
                     GANVLRNLDVDIKKLRLEIEKLVKSGPDMVTMGKLPQTPRAKKVIEYAIEEARSLNHN
                     DVGTEHILLGLLRETEGI"
     gene            complement(622..885)
                     /locus_tag="PLE88_11420"
     CDS             complement(622..885)
                     /locus_tag="PLE88_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77115.1"
                     /translation="MDKTALEQKLKDLLDQIEDFTHPDDRHKFAGHGLEGLLNRPMTE
                     TRQSDPQDWLDSLRLRIQYLLFDLEATRRENSYLKRLLEGPED"
     gene            1325..2200
                     /locus_tag="PLE88_11425"
     CDS             1325..2200
                     /locus_tag="PLE88_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77116.1"
                     /translation="MHLQRIIWAILLIGASCFAQLRNGRFEIPDPNASHQYYTPPKYW
                     DYINYAGLHSSFVPTPHPGHRDPNAVQWSIPSAYEGSNFVLLSTGDTEGPGTDLPTIT
                     AACISQAISVSPGNILMGNYLFGTTDYLPFNDTAVIILIPADSNDGVGEIPIAQMDVL
                     QVGDYKANDGWQSFFVRFDEQTAGDYILYCEVSDDKDTTYQSYLAVDNIRLCLSSSDK
                     GDITQDCAVNYEDFSVLSSAWLCDCQALNDPNSPLYDPNQPCSAADLDRNAVVDTNDL
                     LPMADYWLHNFWTID"
     gene            2371..3396
                     /locus_tag="PLE88_11430"
     CDS             2371..3396
                     /locus_tag="PLE88_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77117.1"
                     /translation="MYFKHSTDQLRGRFRWHYLFAAAAAAAGLCYFIGLSMQQDQPPL
                     TAARFFISAARILFWMGGLTAVFAGLRLLYAAVQNLKTNSEKLDNAVEMLSRQNNLLM
                     QISQASRLSDTAKEIVFRDSEQMELGEAALTRLHQHDFAEAESMIRAMAEQPKYKELA
                     GRLKLMADKYRSATEEGRVNQIIAHIEDLFEQKLWIQAAAQIDNLIKTFPYSEKAKTM
                     PARLQERKDRRKRELLADWDLAVRQKNTDQSIEILKELDLYLTPSEALALRESASTVF
                     KTKLHNLGVEFSVAVTEQNWQKALQTGRIIVQNFPNSRMAAEIRSKMDILLERAASTA
                     KSKPKTP"
     gene            complement(3463..3535)
                     /locus_tag="PLE88_11435"
     tRNA            complement(3463..3535)
                     /locus_tag="PLE88_11435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3500..3502),aa:Lys,seq:ttt)
     gene            3814..4818
                     /locus_tag="PLE88_11440"
     CDS             3814..4818
                     /locus_tag="PLE88_11440"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HOM77118.1"
                     /translation="MKLYSNKTLRFVFFCLSAVLCCLWAAQLTAAKGQVVEPNGLQSP
                     GQDTIESAPSEPNGLSQPDQQTEEENDSFTPKLSTLVVINEAYERIFTPDLITDDGLV
                     RYATLRRLRNDLVTAMRELKTLHPAVLMKMSKEEKIAFWLNTYNACTLQLVIDNYPIQ
                     PKWYMILYPDNSIMQLDKPWTRHFFWISQEQYTLQEIRQELLLDRYKDPRLCFALSYA
                     ARGGAALRNEPYRPQTLDSQLNDQVKKFLSRPDGLRLDKNSNILYLSNVFALQENKKT
                     FLESEWAKVKKFRQRKDEERAWLNFIVQWLPEEDIRYLETAPFTIKFLEYDWHLNELR
                     "
     gene            4941..6149
                     /gene="metK"
                     /locus_tag="PLE88_11445"
     CDS             4941..6149
                     /gene="metK"
                     /locus_tag="PLE88_11445"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOM77119.1"
                     /translation="MTAINIRPGRFNGRGHLFTSESVTMGHPDKVADQISDAVLDAAL
                     QEDPDSRVACETMVTTGLVIVAGEITTKAYLDIPAIVRQTIYEIGYTDAAIGFDWENC
                     AILNSIGRQSPDISQGVTEGQGLHKEQGAGDQGLMFGFACRETPALMPMPIYLAHKIT
                     DRLAVLRKSRKIPWLRPDGKSQVTVEYDCKGKPKRIHTVVVSAQHTEEVKYSTIRKNI
                     IEQVILPVLPPKLVDKNIIYHINPTGRFVNGGPKADCGLTGRKIIVDTYGGRGCHGGG
                     AFSGKDPTKVDRSASYMARYIAKNIVAAGLADACELQLSYAIGVADPVSVLVDTEGTA
                     KVDEAVIERLVRELFPLTPKGIINHLKLKRPIYKETARNGHFGRSHPDFTWEKTDMVQ
                     KLRKAAGLKS"
BASE COUNT         1710 a         1770 c         1535 g         1537 t
ORIGIN      
        1 caatgccctc tgtctccctc agcagcccca gcagaatatg ctctgtcccc acatcattat
       61 ggttcagcga ccgcgcttct tcgatggcat attcaatcac ctttttggca cgcggcgtct
      121 gtggaagttt gcccatcgta accatgtcgg gaccgctttt gaccagcttt tcaatttcca
      181 gacgcagctt cttaatatcc acatccagat ttcgcagcac gttggccccc accccgttgc
      241 cttccttgac cagacctaac aaaatatgct cggtaccgat atattcatgg ttaaaccgct
      301 gggcttcctg attggccaaa gccatcacct tgcgggcacg gtcggtaaat cgctcaaaca
      361 taaatttccc tcacatttca cgtttattcg taaaccgctg tattgtacca tgattggaca
      421 tggtgtcaaa tcttacacgg aactcttaat tatagtgtta tcagaccacg attgttcatc
      481 tttggcgctt atcgctgctt caaaaagaac accttattta tcgacataaa aagacaggta
      541 cttttacaga aaggaaagaa aaactccccc gaagacggaa atgaacccct caaaaatccc
      601 gagtaaaaga ccaatcaggt cctaatcttc cggcccttca agcagccgtt tcagataaga
      661 attttcccgg cgggtcgcct ccaaatcaaa taaaagatat tgaatccgaa gacgcagact
      721 gtcaagccaa tcctgaggat ccgattgtct ggtttcggtc atcggccgat tcagaaggcc
      781 ctccaagccg tgtccagcaa atttgtgccg atcatcagga tgcgtgaagt cctcgatttg
      841 gtccaacagg tcttttagtt tctgttccag tgccgtttta tccatcccta atcccttcta
      901 attcttgcca acctgcccca tatccgcaag aaaaatacca tatcggcctc tgcattctaa
      961 aaaatcctga caaatacatt ttaagactga aaaaacgcct ttttcatccc cctcacaccc
     1021 cccgactcaa tgttaattta ttcacaactg tttattattt tcaacataag accggtaaaa
     1081 agttttcttg gtttttatat cctcttgatt aacagataat tacagcaatg ttgcctattt
     1141 taatcaccca atttatccgt aaaaaaccaa atgtatttaa aagatagtgg ttattttcag
     1201 cgtatataag acgattaact aataggactt tggtataata gtatcataaa tcaggacagc
     1261 ggctatccaa tagcagatgg ggtttaaggg gtgtttgttt aaaatctgga cttatcatta
     1321 taaaatgcat ttacaaagaa tcatttgggc cattttgctg attggcgcca gctgtttcgc
     1381 acagctgcgc aatggccggt ttgaaattcc cgaccccaac gcaagtcatc aatattatac
     1441 gccccccaaa tactgggatt atataaacta tgctggattg cacagttcct tcgttcccac
     1501 accccatccc ggacatcggg accccaatgc tgtccagtgg agcatccctt ctgcttatga
     1561 aggaagcaat tttgtccttt tatcaaccgg agatacggaa ggaccgggaa ccgatttgcc
     1621 caccattacg gccgcctgta tttcccaagc catttcggta tccccgggca atatcctgat
     1681 gggcaattat ttgttcggca ccacagacta tctgcctttt aacgatacag cggtcatcat
     1741 cttgattccc gccgattcaa atgacggcgt cggcgaaatc cccattgcac agatggatgt
     1801 gctgcaggtg ggcgattaca aggccaacga cggctggcag agtttctttg tgcgctttga
     1861 tgaacagacc gcaggcgact acattctcta ttgcgaagta agcgacgaca aagataccac
     1921 ctaccaaagc tatctggcgg tcgacaacat ccgcctgtgc ctcagcagtt cggacaaagg
     1981 cgatatcacc caagactgtg ctgttaatta cgaagatttt tctgttctgt ccagcgcttg
     2041 gctgtgcgac tgtcaggcgc tcaacgaccc caattccccc ttgtatgatc ccaatcagcc
     2101 ctgctctgcc gccgatctgg acaggaatgc tgttgtggac accaacgacc tgctgccgat
     2161 ggctgattac tggctgcaca acttctggac cattgattga ttcctctttc ggtcctcgcc
     2221 tgccgatgcc gccgtgccgg atcagcacgc cttgacggcc gaagtattta tttgacaaaa
     2281 gccggccgaa tggataggat gaaatactgg aaacggatga taaaaccacg gcacagcaaa
     2341 gcgatacgat tttgagtaag gaggccgacg atgtatttta aacacagcac tgatcagctt
     2401 aggggacggt tccgctggca ttatctcttt gcagcagcag cagcggcagc cggtctgtgc
     2461 tattttatcg gcctgtcgat gcagcaagac cagcccccgc ttacggccgc acgctttttt
     2521 atctctgccg cacggatttt gttctggatg gggggcttga cagccgtctt tgccggcctg
     2581 cggcttctgt acgcggccgt gcaaaacctc aaaaccaaca gcgaaaaact ggataacgcc
     2641 gtggagatgc tgtcccggca gaataacctg ctgatgcaga tttcccaagc atcccgcttg
     2701 agcgatacgg ccaaagaaat cgtatttcgc gactcggagc agatggagct gggagaggcc
     2761 gccctgaccc gtctgcacca gcatgacttt gctgaggccg aatccatgat tcgtgcaatg
     2821 gcagaacagc ccaaatacaa agagctggcc ggccggctga agttgatggc tgacaaatac
     2881 cgcagcgcca ccgaggaagg acgcgtcaat cagattatcg cacacatcga agacttgttt
     2941 gagcagaaac tctggataca ggccgctgca cagattgaca atctgataaa aacattcccg
     3001 tattcggaaa aagccaagac aatgccggca cgcctgcagg agcgcaagga ccggcgcaag
     3061 cgggagctgc tggccgactg ggacttggct gtccgccaga aaaataccga ccaaagcatc
     3121 gagatactca aagaactgga cctgtacctg acccccagcg aagcattggc cttgcgggaa
     3181 tctgccagca ccgtctttaa gaccaagctg cacaatctgg gcgtcgaatt ttccgtagcg
     3241 gtaaccgagc agaactggca gaaagccctt caaacagggc gaatcatcgt ccaaaacttc
     3301 cccaacagcc gaatggccgc tgaaattcgc agcaagatgg atatcctgct ggaacgtgcc
     3361 gccagcacag caaaaagcaa acccaaaacg ccgtaggcac ccggcgcagg aaacaaccat
     3421 gcaaagaggg ttgctgggca aaaaagcggg cttctgaagc aatgaaggtg ctgggactcg
     3481 aacccaggac caacggctta aaaggccgtt gctctaccga ctgagctaca cctcctaaaa
     3541 aaacgtccag aaatactatc tatatataca attttatcaa gtaaaatacg ctaaaaatgc
     3601 ttattttttt tattttttac tgcccctgcc ccagcgtcta ttaagcagcc gggctttcta
     3661 accgagaaaa aatatagaca acagcaggaa agattcttct cgatagaatg aactgctttg
     3721 gtataaataa agtattatag tttacgataa ttttattctt ttatttgtaa atgttgttcc
     3781 gtctgttttt ttcggctgta atcaggcaaa acagtgaaat tatattctaa caagacatta
     3841 cgattcgtct ttttctgtct gtctgctgtc ttatgctgcc tgtgggctgc gcagctgacg
     3901 gcagcaaagg gacaagttgt cgaacccaac gggcttcaat cccccggtca ggacacgata
     3961 gaaagtgccc cttctgaacc caacggcctg tctcaaccag accagcagac ggaagaagaa
     4021 aacgacagtt tcaccccgaa actttccaca ttggtcgtca tcaacgaggc ctatgaacga
     4081 atatttaccc ccgacttaat caccgatgac ggactggttc gatatgccac attgcgccgg
     4141 ctgcgaaatg atttagtcac ggcgatgcgg gagctcaaaa cactgcatcc ggcggtgctg
     4201 atgaaaatgt caaaggaaga aaaaatcgct ttctggctca atacctacaa tgcctgcacg
     4261 ctgcagctgg ttatcgacaa ttaccccatt cagcccaaat ggtatatgat tctctatccc
     4321 gataacagca tcatgcagct ggacaagcca tggacgaggc attttttctg gatcagccaa
     4381 gaacagtaca cccttcagga aatccgtcag gagcttcttc tggaccgcta caaagatccg
     4441 cggctgtgct ttgccctgtc gtatgcggca cgcggcggcg cagctttgcg caacgaacct
     4501 tacaggcccc agactctcga ttcccagctg aatgaccaag tcaaaaaatt tctcagccgc
     4561 ccggacggcc tgcggctgga taaaaacagc aatatcctgt atttgtccaa tgtctttgcc
     4621 cttcaggaaa acaaaaaaac cttcctcgaa tccgaatggg ccaaagtcaa aaagttccgc
     4681 cagcgaaaag atgaagaacg ggcgtggctg aattttattg ttcagtggct cccggaagaa
     4741 gacattcgct atctcgaaac agcgccgttt accatcaaat tcctcgaata cgactggcat
     4801 ctcaacgaac tccgctaaac aggccggcac cagcgcttct ttgtccccct ttttttagcc
     4861 caggaatctt cttgccaagt acccccggca ggagtatcat acaggtttat tttattattt
     4921 attttatccg ggagataaac atgacagcaa tcaatatacg gccgggccgt ttcaacgggc
     4981 ggggacatct gtttaccagc gaatcggtaa caatgggaca tcctgataaa gtggccgacc
     5041 agatttccga tgcggttctg gatgctgcac tgcaagagga tcccgacagc cgcgttgcct
     5101 gtgaaacgat ggtaacgacc ggtctggtca ttgtggcagg cgaaatcacc accaaggcct
     5161 atttggatat tcccgccatc gtccggcaga ccatctacga aatcggctac accgatgcag
     5221 cgattggatt tgactgggaa aactgcgcca ttctcaacag catcggccgg cagagtcccg
     5281 atatctcgca gggggttacc gaaggacagg ggctgcacaa agaacaaggc gccggcgatc
     5341 agggactgat gttcggattt gcctgccggg aaaccccggc cttgatgccg atgccgattt
     5401 atctggcgca caaaatcacc gaccggctgg ccgtcctccg caaaagcagg aaaatcccat
     5461 ggctccggcc ggacggaaaa agtcaggtaa ccgtcgaata cgactgcaaa ggcaagccca
     5521 aacggatcca caccgtcgtc gtttcggccc agcacaccga agaggtcaaa tacagcacca
     5581 tccggaagaa cattatcgag caggtgatac tgccggttct gccgccgaag ctggtggaca
     5641 agaacatcat ctatcacatc aatccgaccg gccgttttgt caacggcggc ccgaaagccg
     5701 actgcggcct gacgggacga aaaatcattg tcgataccta cggcggacgc ggctgccacg
     5761 gcggcggcgc attcagcggc aaagacccga ccaaagtgga ccgcagcgcc agctatatgg
     5821 cacgctacat cgccaaaaac attgtcgcag cgggtctggc cgatgcctgt gaacttcagc
     5881 tgtcctatgc catcggggtt gccgatccgg tttcggtatt ggtggatacc gaaggcaccg
     5941 ccaaagtgga tgaggccgtc atcgagcggc tggttcggga gcttttcccg ctgacgccta
     6001 agggaatcat caaccacctc aaactgaaac ggccgattta caaagaaacc gcccgcaacg
     6061 gacatttcgg ccgcagccat cccgacttta catgggaaaa gacggacatg gttcaaaagc
     6121 tccgcaaggc cgccggcttg aaatcgtagc ccagccggca caaatgccga aagccgtctg
     6181 tcctgcaaac tgctgcctgc cgcaagttct tgctgcgctg tcggcctgcg gaagggtttc
     6241 tgcgcagcca gcttggcggt ctttcgcgga tacgcctgcc agaactgctc aatgagcgca
     6301 tcctcacgca cacgcccccc ctgtgggggg actagagggg ggtatataat attatttaaa
     6361 aatattctgt ggctcacttt tgcgccagtt gtgtgagttt gggaaccgct gttcgggctc
     6421 attgtgaacc ctgtgaggcc tgttttcacg ctgttgaggg tcattttcga accggttgaa
     6481 cgattcttga tgccatcggg gctcactttg cagccagttg gattgttttg cgcgctgttg
     6541 gggctcactt tc
//
LOCUS       DAOTBO010000128         6523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10034_length_6523_cov_6.576685, whole genome shotgun sequence.
ACCESSION   DAOTBO010000128 DAOTBO010000000
VERSION     DAOTBO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_6523_cov_6.576685"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLE88_11450"
     CDS             complement(<1..581)
                     /locus_tag="PLE88_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77120.1"
                     /translation="MQKGVFQAEVLSNQQIRRCYYRLNLQFDSKGSLLFSSVVPGQFL
                     ELDVSSISLPPASLIPEHLRETAAKQVFLRRPFSFSDVIKVNDAEGTVVRVEILYCVL
                     GPATVRMMALKSKDKISVLGPLGNGFSIPQNLQTAILIAGGMGSPPLLHLASYLKHSL
                     PQCRIVSFVGAKSCEDLPFTVRIGNFKGLVLEE"
     gene            complement(1053..1928)
                     /locus_tag="PLE88_11455"
     CDS             complement(1053..1928)
                     /locus_tag="PLE88_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77121.1"
                     /translation="MKNFPAAQWMLCMAAFGILVLSAAAQDESKPKEPPQPASGETKS
                     REITSDIWHEISLESDDIKLTEKKVQEILEELKKSSPQQAEQLAALRQSDPSKFIDAI
                     REEIRKHTQSEKPAAGTEDKSSQWKEQLERRHELFLEWFQKGYANEFGELVKLRESNP
                     EKYVQRVLDLMKVYEPIQRAERISPELASVMKKELELQKQQDELLVQIRLAAKDQQPK
                     LTEELKRLVSTQFDAIVSEKQIRCELLRKRLESLTQQLEAQAAELELLKKNKSQTVED
                     RIKELIERNEKINWD"
     gene            complement(1934..2452)
                     /locus_tag="PLE88_11460"
     CDS             complement(1934..2452)
                     /locus_tag="PLE88_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77122.1"
                     /translation="MMNNKEHLEELLLQFMDRKQAVEAAREISDADRLFDAQPLPALS
                     RDRLAAVQAKVQLAIEQQRRRASEIRWFSAAASIVAAALLVGLYVLNQADKDIQIPAS
                     LYYATALDWNRMNRSDDSALSQIENELRDVAESMKSLSEDTFKPVNTLSLDTMELDEI
                     ESLVRNTEFWKG"
     gene            complement(2449..2982)
                     /locus_tag="PLE88_11465"
     CDS             complement(2449..2982)
                     /locus_tag="PLE88_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM77123.1"
                     /translation="MTESSRLDKLINRCKKGDPEAFNTLIECYADRCYAYFYRLTGRA
                     DISEELLSDLFIRLFRKMRSYESGSFDKWIFTVASNLFRDSLRRQYRQKRLIEEKTEL
                     LRQSESADKSNPEVFDKLQLALEKLDTETAELIVLRYYSQMSFKELAQMRQEPIGTTL
                     SKVHRGIKKLRQIMDDL"
     gene            complement(3695..5500)
                     /locus_tag="PLE88_11470"
     CDS             complement(3695..5500)
                     /locus_tag="PLE88_11470"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOM77124.1"
                     /translation="MKHYQVHFKPDDVTAIVHAGATLLEAAGQAGIILANPCGGIGRC
                     GKCKVQLLPSGKEVLACQFRIDHNLTVQVPDSSRFFLQKILEQGTEHHTLAEPAILKI
                     FIDTPPASIDDFCELLSDKIQLPFTLQDSINTQAAESLNLWRKTGVTVIFALSSVGND
                     KPTYRVTGFETGNTTDVLYGIAVDIGTTTIVAHLLDLNTGRRAATVSACNPQSQYGAD
                     VISRIGYAETDEGLLQLHRSVIDCLNELIHKAATAGSIDCSCIYEIAVVGNTTMNHLF
                     LNYPVKQLGQAPYRPYSLLAADRSPSQLQITINPAGNIHTAANIAGFIGSDTVASVLA
                     CKMEAASENILLVDIGTNGEIVLAASGHLLAASCAAGPALEGAGIEFGSRAQKGAIER
                     VLYDGCDIDVDVIESDTALTICGSGLIDAAAVLLEADIIESSGQFRTPETLSPFTAPS
                     IRNRLIAYKGKPAFVLAGTYKNQQWKNPVLLTQKDIRQLQLAKAAIRTGIELLLKEAK
                     IDAENIGKVLLAGAFGNYIQKDSAVRIGLLPPIPLEKIHFVGNAAGTGAEMILISSQM
                     RQQAVRLAQSIRYVEIARQNSFQSVFSESLLFPEK"
     gene            complement(5647..>6523)
                     /locus_tag="PLE88_11475"
     CDS             complement(5647..>6523)
                     /locus_tag="PLE88_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOM77125.1"
                     /translation="VSALHTACRAFTEQDEHVLTFTPVYPPFLSAPRLSGRSLIVCPL
                     NNENGHYRIDFHRLQKILTDKTRLLILCSPHNPIGRVWTKDELLKIIEICSERNILLC
                     SDEIHCDLILDKSVQHIPTAALMHGAENRIITLMSPAKTFNLPGLSCGFAVIPDIQLR
                     RRFIQTASGIVPHVNVFGYTACAAAFSEGLPWLQEVLDYLRENHRILYETVNETPGLS
                     MGNVQATYLAWIDVSRLNLSKPAAFFEQAGVGVMDGADFGQAGFVRLNFACNRDILLT
                     ALDRIKQAVTNHVCE"
BASE COUNT         1736 a         1448 c         1449 g         1890 t
ORIGIN      
        1 aattcttcca gcacaaggcc tttgaaattc ccaattcgga cggtaaacgg caaatcttca
       61 caggattttg caccaacaaa gctgacgatg cggcattgcg gcaaactgtg ttttaaatag
      121 ctggccagat gaagcagggg aggagagccc attccaccgg ctataagaat tgccgtctgc
      181 agattttgag gaattgaaaa tccgttgcct aagggaccaa gtacgctgat tttgtctttg
      241 gatttcagcg ccatcattcg gacagtagct gggcctaaca cacaatagag gatttcaact
      301 ctaacgacag ttccttctgc atcatttact ttgattacat cggaaaaact gaagggtctt
      361 cgtaaaaata cctgctttgc ggcagtctcc cgaagatgtt ccgggattaa agatgcagga
      421 ggtaaggata tgctgcttac gtcaagttca agaaattgtc cggggaccac gctgctgaaa
      481 agaaggctgc cttttgagtc aaattgaagg ttcagtcggt aataacagcg tcgaatttgc
      541 tggttgctta agacttcagc ctggaacact cctttttgca tctaatccct ttttgacaaa
      601 taagttacaa aaataaagct tccggaacat tcatccaccg acattccgga agcccgccac
      661 ggcaaagaaa gccgttaaat tcgcttttta ctatacaaaa gagtatagca gtcatacaaa
      721 tgtatgtcaa tcaaactgaa ccattttatt tatggtaaaa cattgtccac ataagacaat
      781 aattgtatcc cttttattat taataactta tataaaaaaa ggatgaaaaa aatgtataaa
      841 aatagttctt tctttttccc cgtccagctt tgcagaagga gaaatgtttt tgattaactt
      901 atttgctatg catagaagct cttgtttaag caattcgtgc ataacgactt acagcaggaa
      961 gcgaaacggg catcatcaat aaatctatat atgatttgga gatattttgc ggtttgcaag
     1021 gcgtcatctg gctctgagag aatgttcgct gcctaatccc agttaatttt ttcgtttcgt
     1081 tcaatcaatt ctttgattct atcttcgact gtttgactct tgtttttctt aagtaattcc
     1141 agctcagccg cttgggcttc gagctgctgg gtgagagatt ccagccgttt ccgcaggagt
     1201 tcgcagcgaa tctgcttttc cgatacaata gcatcaaatt gggttgaaac gagccgtttt
     1261 aattcctccg tcagtttcgg ctgctggtct ttggctgcca gccgaatctg cacaagcagt
     1321 tcatcctgct gtttttgaag ttccaattcc tttttcatta ccgaggccag ctcggggctg
     1381 attcgttctg cacgctgaat cggctcatac actttcatca gatccaaaac acgctggaca
     1441 tatttttcgg gattggattc ccgaagcttg accagttcgc cgaattcatt agcataccct
     1501 ttttggaacc attccaaaaa cagctcatgc cgtcgttcca gctgctcttt ccattgggac
     1561 gatttatcct ccgtccctgc agctggtttt tcgctctgtg tatgtttgcg gatttcttcc
     1621 cgaatggcat cgataaattt tgaaggatcg ctctgacgca aagcagccag ttgttcggct
     1681 tgctgggggc tgcttttttt aagctcttca agaatctcct gcactttttt ttctgttaat
     1741 ttaatatcat ctgattccag cgagatctca tgccagatat ccgatgtaat ttccctgctt
     1801 ttggtttccc cagaagcggg ctgcggaggt tccttcggtt tgctttcatc ttgagcggca
     1861 gcagacaaaa ccagtattcc aaaggcggcc atgcagagca tccattgtgc agcagggaaa
     1921 ttcttcattt cgtttatcct ttccaaaact cggtatttct gaccaaagat tctatttcat
     1981 ccaattccat tgtatccaga gaaagagtgt tgaccggctt aaaggtgtct tccgacaggc
     2041 ttttcatcga ttcggccaca tcgcgcagct cgttttcaat ttgagagagg gcagaatcat
     2101 cagaacggtt cattctgttc caatctaaag ctgtagcata atacagagaa gcagggattt
     2161 gaatgtcttt atctgcttga ttgagaacat ataatccaac aagaagtgct gcagctacaa
     2221 tagatgcggc ggcgctgaac catcggattt ctgaagcccg gcgtcgttgt tgttcaattg
     2281 ctaactgaac ttttgcttga acagcagcta acctgtccct cgagagggca ggcagaggtt
     2341 gagcatcaaa cagacgatcg gcgtcggaaa tctcacgggc cgcttcgaca gcctgctttc
     2401 ggtccataaa ctgaagcagc agttcttcta aatgttcttt attattcatc ataagtcatc
     2461 cattatctga cgcagttttt ttatgccccg atggactttg gataatgttg taccaatagg
     2521 ttcctgtcgc atttgggcta attctttaaa gctcatttga ctgtagtagc gtaaaacaat
     2581 taactcggct gtttctgtat cgagtttttc cagagccaac tgaagcttat caaaaacttc
     2641 aggattggat ttatctgcag attctgactg tctcagtaac tctgtttttt cttctatcag
     2701 gcgtttctgt ctatattggc gacgaagcga gtcccgaaac aaattcgatg caacggtaaa
     2761 tatccatttg tcaaaagacc cgctttcata agatcgcatt tttcgaaata accggataaa
     2821 cagatcgctt aacaattctt cactgatatc tgcccgaccg gttaagcggt aaaaatatgc
     2881 ataacagcga tctgcatagc attcaatcag cgtgttaaat gcttccggat cgcctttttt
     2941 acatcggttg attaacttgt caagtcttga cgattcagtc atgacattac gacttcttat
     3001 gaaaagacgg atacacttgt ctgcttattg cataaaacgg ctcttgaagc ggcaaaatag
     3061 gatttttaat aaaggccatt aaaggagaca atccttccct ttgaatgatt tcaataaccg
     3121 gccgcttatt ctctattctg gttcgtctgt gtcttcaaat ttccacttgt ctgcttcatc
     3181 gatgtcgtca agttcgcgtt taacatactt gagaatcttg tcttgaattg gtgccatttt
     3241 gttttgccaa gtaatcatag tctgataatg agtaaccaga agatcaagac gcaagagctg
     3301 ccatttcaag agacattcag gctcttttat gttctgaaaa gggtcgcatc tgaatgtcct
     3361 gcggccgtgt tcatcctgtg aataatattc acaatccttg cattgaatca tgagttattc
     3421 ctctcgaaaa atacttgcat tctcattgta aacttttata ataaccctca aaaatgattt
     3481 tgaaaagaaa gaaagtttac caggagaaat aaatggctaa gccgagacga agaagaaaga
     3541 ttggcagcaa gaagcgtaag acaaaatgga aagttcgtca taagataaca taatattttc
     3601 attgcataag gaaacagagt gccttttgag aggcattcta aaaattgcat tcaaggccgc
     3661 aaaagaagag ggcaagattc ttcttttgcg gctttcattt ttcagggaac aggagggatt
     3721 cgctgaagac cgattgaaac gagttttgtc tggcgatttc aacataacgg atcgactgtg
     3781 ccagtcgaac agcctgctgc cgcatctgac tgctgataag aatcatttct gctccggtac
     3841 cagcggcgtt cccgacaaaa tggatttttt caagcggaat aggcggcaat aggccgattc
     3901 ttacagcact gtctttttgg atgtaatttc caaaggctcc tgccaacagg actttaccaa
     3961 tattttctgc atctatcttt gcctctttta acagcagttc aatgcctgta cgaatggctg
     4021 ccttggcaag ctgcagctga cggatgtctt tctgggtcag cagaacagga tttttccatt
     4081 gttggttttt atacgtgccg gctaaaacaa atgccggttt acccttatag gcaatcaggc
     4141 gattacggat agatggtgcg gtaaatggtg aaagagtttc tggagtccgg aattgcccgg
     4201 atgattcaat gatatctgcc tctaataaaa cagctgccgc atcaatcagt ccgcttccgc
     4261 agattgtaag ggctgtatcg gactcaatga catcaacatc aatatcgcat ccgtcatata
     4321 gaacccgctc gatcgctcct ttttgggccc ggcttccaaa ttcaatccct gctccctcca
     4381 atgccggacc agcggcacag ctggctgcca gcaaatgacc acttgctgcc agaacgattt
     4441 ccccattggt cccaatgtcc actaaaagga tattttctga agcagcctcc atcttgcagg
     4501 ccagaacaga tgcgactgtg tcagaaccta taaagccggc gatattagct gcggtatgga
     4561 tatttccggc cggattgatt gtaatttgta gttgagatgg gctgcgatcg gctgcaagca
     4621 gggaataagg ccggtaaggg gcttgtccga gctgcttaac cggatagtta agaaaaaggt
     4681 gattcattgt cgtattgccg acaacggcaa tttcatagat acagctgcag tcaatacttc
     4741 ccgccgttgc tgctttatga atcaattcgt tcaggcaatc gatgacggat cggtgcaatt
     4801 gcagcaatcc ttcgtctgtt tctgcatacc caattctgct gataacgtcc gcgccatatt
     4861 gagactgggg attgcaggca gaaacggtag ctgcgcggcg gcctgtattt aaatccagca
     4921 gatgtgctac gatggtggtt gtgccaatat caacggctat tccataaaga acgtcggtgg
     4981 tattgcccgt ctcaaatccg gttactcgat aggttggttt gtcatttcca acggaagata
     5041 atgcaaaaat tacggttact cccgttttcc gccagagatt cagagattct gcggcttgtg
     5101 tgtttataga atcttgcaga gtaaacggaa gctgaatttt gtcggataac agctcacaaa
     5161 agtcatcgat ggatgcgggg ggagtatcaa tgaagatttt taatattgca ggttctgcaa
     5221 gggtgtggtg ttcggttcct tgctcaagaa ttttctgcag gaaaaacctg ctggaatctg
     5281 gaacttgaac agttaaattg tggtcaatcc ggaattggca ggccagaact tctttgccag
     5341 aaggcagaag ctgaactttg catttgccgc atcggccgat gcccccgcag ggattagcaa
     5401 ggataattcc cgcctgacct gcggcctcca gcagggttgc tcctgcatga acaatggccg
     5461 tcacatcatc cggcttgaaa tgaacttgat aatgtttcat gccttatttc cagcttgcat
     5521 taaaaaacag cattatatga acaaagcgta agatatccta taatcggacg gctatatttt
     5581 ttaatggttc tgaacaactg tatggtgtaa gggcaaagcc caaacctttc aatttttttg
     5641 tgatgcttat tcgcatacat gattggtgac ggcctgtttt atccggtcta aagctgttaa
     5701 gagaatatcc cgattacaag cgaaattaag ccgtacaaat cctgcctgcc caaagtcggc
     5761 gccgtccatc acgcccacgc cggcctgctc gaaaaaagcc gccggcttcg agaggttcag
     5821 tctgctgaca tctatccacg caagataagt ggcttgcacg ttgcccatcg ataatcccgg
     5881 tgtttcgttc accgtttcat aaagaatacg atgattttcg cggagatagt ccagcacctc
     5941 ctgcagccac ggcaatccct cggaaaaagc agctgcgcag gccgtgtagc cgaagacatt
     6001 tacatgcggg acaattccgc ttgccgtttg aatgaatcgg cggcgcagct ggatatcggg
     6061 aatgacggca aatccgcagc tgagtccggg caaattaaat gtttttgcag gcgacatcaa
     6121 tgtaataatt ctgttttctg caccatgcat taacgccgca gttggaatat gctgtacaga
     6181 tttgtctaaa atcaaatcgc aatggatttc atcggaacac agcagaatat ttcgctcact
     6241 gcagatttct attattttca gcaattcgtc tttggtccag actctgccga tgggattgtg
     6301 cggactgcac aggataagaa ggcgagtttt gtcggtcagg attttctgca gtctgtgaaa
     6361 atcgatgcgg taatgtccat tttcattgtt cagcggacat actataaggc tgcggcctga
     6421 aagccgagga gcggatagaa agggaggata aacaggtgta aatgtcagaa cgtgctcatc
     6481 ttgttctgta aatgcccggc atgcagtatg aagcgctgac acc
//
LOCUS       DAOTBO010000129         6513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10053_length_6513_cov_3.387426, whole genome shotgun sequence.
ACCESSION   DAOTBO010000129 DAOTBO010000000
VERSION     DAOTBO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10053_length_6513_cov_3.387426"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1643
                     /locus_tag="PLE88_11480"
     CDS             150..1643
                     /locus_tag="PLE88_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77126.1"
                     /translation="MKRMLFLLGIVLMMGGLVQAAAYYFEGTQDNLWSNLNNWTVEGS
                     VPSVLPGAADNVRIRSSCLLNYDAGIINSLQIGSGGAGDLTVDGGKIDAAKGVSGTAE
                     MGAVGWDAPGSLTINNNGEVIIRNDTTIGCWANKGTVTVNSGRFFVPAALFLAIYDNN
                     GVFVDSEVKICKSGIVECYYITVKGGVIDVWNGRFCPWYSDAAVSDLIAAGKIIGFGG
                     QGTVSFDIVPWPHTVTAKHNMHPSPDFYETVVAGDVALSWTNMEPNTPGDDVYVDVWF
                     GTEPNKLGANYAQVVTAGQNTTTVTVNASDVPKTYYWQVDSYLYGDPAVVHYSNSDPN
                     AYPIIESALFEFYTTYDSAPIVNAGVDMITWINEPVQLAPTVIDDGVSSLTYLWTTAP
                     ETEPNVIFSDPAAEAPLVTVDSALGAVTLTLTVQDEFNAPVSDTVVINVYGSPCSAAV
                     DGPGLDPADQQLDLYFDCRIDLQDVAVLAARWLENYELTAPIPVPAP"
     gene            1792..2559
                     /locus_tag="PLE88_11485"
     CDS             1792..2559
                     /locus_tag="PLE88_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77127.1"
                     /translation="MKNLKFCVVFLLAAAMMTNAQIWYGLDDGDVWSDASNWIGGSGG
                     VPGVSDWAEIIYDFPKTCILDSDAGTINTLHISAGGTGASMLIKNGGRLTVSGGDVSP
                     GWNNNGTLTIETGGSLTANCMFTVAMYDDTDSFARLILNGGTLTVNNAFYHGIYFDGS
                     STVDIRTEINAGVLDVDALILDRGIMNITNGTVIVAGDVLGLVANWVSAGRLTAFDGA
                     GTIVAQLGNGYTEITAVPEPSMLVLLALGTLTLHRKR"
     gene            2663..5965
                     /locus_tag="PLE88_11490"
     CDS             2663..5965
                     /locus_tag="PLE88_11490"
                     /inference="COORDINATES: protein motif:HMM:NF027717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5010 domain-containing protein"
                     /protein_id="HOM77128.1"
                     /translation="MLMQRSACFKHISFFLIILFVSVLSAAGYASSWNGHGQNDLWSN
                     ASNWIGGIPGPADYARWNLANTTPCYLNHNAGTIDTLLVSEYQQGHLIIQDGGSLTIT
                     NYASIMNRSGSITVQPNGLLHVIGDLIIGQWDDLPDDEMILTMNGGTIVVEAVLRFGF
                     YFDGVNGQYTVKTHAQLNGGELYAGSVSGLQADRGTIDITHGKLILNGNHKAAVQNWV
                     TSGFLTAYDGNGIITYDYDRTNQGKTTIWAAAPVNGDVDGDFDVDFDDLILFVGDWLS
                     QECGVHSDFTGDCKVNMQDLAQMSENWFYGVSLAAFDPNPADGQVIMPSQLFWKAPPF
                     IDSFDIYFGMNYMAVLNADHESPEFQGTQSEKNFAPGLLYPDEHYYWRIDVVLGDKKY
                     PGTVWSFSTVDMENAAGFIGATCGWQYSAQLEGPLTYPNQTNISLYNPQRGNPTATWE
                     DWAAQITQAGLDFICPNLTGSWPNTGSPPADMAPLVTALNNLGNVTKFAIFDDNAASW
                     CAQWNMAEGRGFWYAEPFDISDPANWVFIYDYNYKLFYETVPDAQRFKIDGRPVIWIW
                     TGNPYFVANSQGNLSRAMLWVRQRCMEDFGFNPYIVGNRDFISNDSTCANPGVLDATH
                     SWFGPPNDPWTLSVFNGVKIGVAVAQFQNSSNPPGFLDPDHGNLLNEGLHHTVGAGAL
                     MTLLEGFTDYEEDAALFRVRNLDENGNPLSYEQTYYDYPNQRLNIIRKHSRHPFPAEL
                     KLEAEGCDTYGGAAGGGTPRLYRNGNIGIEPTQDEGGGFSIGWIQPNEWFQWEEVPME
                     GSGTLQVRVAANNDAGRLHFVIDGQVKPAVALPDTGGFQSWATVDTPPETFAPGSYHT
                     VRLICETSGMNINWIRWVAGGTDNAPPRVVINSPVNNSTYAEPAAVTIEAAAFDEDGT
                     ISKVDFYHGSTLLGTDTAAPYSFTWNNVPAGDCGSYRLSAKAVDNQGAVSTSLPVFMN
                     VTPLSLNKPAYASFVVNRNVPSRAADNDLSTRWESAYSDPQWIYIDLGAVYDIGKIVL
                     KWEAAYASSYQIQISDDAVHWTTVYSTTVGDGGYDVLNVSAAGRYVRMYGTTRGTIYG
                     YSLYDFKVYMAY"
     gene            6093..>6513
                     /locus_tag="PLE88_11495"
     CDS             6093..>6513
                     /locus_tag="PLE88_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOM77129.1"
                     /translation="MDVYQRTCTQTGFFASLQAALTIFLLVAGTAAAEVTFSGSAPAP
                     DSELALWYRQPAADWESQALPIGNGYIGGMVFGGVAQEQISLNEKTLWSGGPGEYPDY
                     TGGNNTDRTVYLANIRKMLEQKNYAAADAYAENLKGIS"
BASE COUNT         1575 a         1535 c         1756 g         1647 t
ORIGIN      
        1 cgcaacgccg gaggaaacca gatattatgc gggcaagcgg ttctggtagc cggcgggctt
       61 gtaggggttg attccttctg aagttcaagc aaaagtaagg cgttataaaa gtaaacagaa
      121 aatgaatgtt acgggaaagg agaatggcga tgaaacgaat gttgttcctg ttaggaattg
      181 ttttgatgat gggggggttg gtgcaagcgg ccgcatatta cttcgagggg acccaggaca
      241 atctgtggag caaccttaat aactggacgg tggagggaag cgtgccttcg gtcttgcctg
      301 gcgcggcgga caacgttcgt atcaggtcgt cctgcctttt gaactacgat gccggaatca
      361 ttaacagtct gcaaattggc tcaggtggag ccggcgatct cactgtagat ggcggtaaaa
      421 ttgatgccgc caaaggggtc tctgggacgg cggaaatggg agcggttggt tgggatgctc
      481 ccggttcgct gacgatcaac aacaatggcg aagttattat acgcaacgac acgacgattg
      541 gatgctgggc aaacaagggc acggtaacgg ttaacagcgg cagatttttt gtgccggccg
      601 cactgttttt agctatttac gataacaacg gtgtttttgt ggattctgaa gtcaagattt
      661 gcaaaagtgg tattgttgag tgctactaca ttactgtcaa gggcggtgtg atcgatgttt
      721 ggaatgggcg attctgtcca tggtacagcg atgcggctgt ttctgattta attgctgccg
      781 gaaagatcat tggttttggc ggccagggaa cagtaagttt tgatattgtc ccatggcctc
      841 acacagttac cgctaagcac aacatgcacc cgtcgccgga tttttatgaa acggtcgtgg
      901 ccggtgatgt tgctctgagc tggacgaata tggaaccaaa cacccccggt gatgacgttt
      961 atgtggatgt ctggtttgga acagaaccga ataaactggg tgcaaattat gcacaggttg
     1021 ttacagccgg tcaaaataca acgactgtta ccgttaacgc atcggatgtt cccaagactt
     1081 actactggca ggtggattcg tatctttacg gcgacccggc ggttgttcat tatagcaaca
     1141 gcgacccaaa cgcttatccg ataattgaaa gcgccctatt tgaattctac accacatatg
     1201 attccgcccc catagtcaat gccggcgttg atatgattac ctggattaac gagccggtcc
     1261 agttggctcc gaccgtcatt gatgacggtg tcagttcatt aacctatctc tggacaactg
     1321 cgcccgaaac agaacccaat gttattttct cagatccggc ggcagaagcc ccgcttgtca
     1381 ctgtggacag tgcactgggt gcggtcacgc tgacattgac tgttcaggat gagtttaatg
     1441 ctcccgtatc tgacaccgtc gtgatcaatg tttacggaag tccctgttcg gctgctgtcg
     1501 atgggccggg actggatcct gccgatcaac agctggattt gtattttgac tgccgaattg
     1561 atctgcagga cgttgccgta ctggccgcca gatggctgga aaattatgaa ttgacagcgc
     1621 cgatcccggt tccggctccc taacaggagg tcggcagcaa agcatatctc tccgatacgc
     1681 gggctgattt tgcgggtcga taaagctgaa atgggatgtg aagtgtgtga aggaaaacct
     1741 gatgcatggc tatgtataaa attaaattag tttattttgg aggttggaaa gatgaaaaac
     1801 ttgaaattct gtgtggtttt tttgttggct gcagccatga tgacaaatgc tcaaatctgg
     1861 tatggtctgg atgacgggga cgtctggagt gatgcctcca actggatcgg cggctccggc
     1921 ggagtccccg gcgtttcgga ctgggcggag ataatttacg atttcccgaa aacctgcatc
     1981 ctcgacagcg atgcaggcac catcaacacc ctccacatct ctgccggcgg caccggtgct
     2041 tctatgctca tcaaaaatgg cgggcgtctg acggtttcag gcggcgatgt gtccccggga
     2101 tggaacaaca acggtactct gactattgag accggcggct cattgacagc caactgtatg
     2161 tttactgtcg ccatgtacga cgatacggat agttttgccc gcctgatcct gaatggcggg
     2221 accttgactg tcaacaacgc cttctatcat ggcatctact tcgacggcag ttccaccgtg
     2281 gacatccgga ccgaaatcaa tgccggcgtt ctggatgtgg atgccctcat tctggaccgc
     2341 ggcatcatga acattaccaa cggaaccgtc atcgtggcgg gtgacgtttt gggtttagtt
     2401 gcgaattggg tttctgccgg tcgcctgaca gcgtttgatg gtgcaggaac gatcgttgcc
     2461 caactgggca atggttacac agaaatcacc gcggttcccg aaccgtccat gctggttctg
     2521 ctggctttgg gtacgctgac gctgcaccgg aaaagataaa tagggttgct ttttaagacc
     2581 tgccggatat tcccggcagg tctttctatc cttagaggaa tagaatgtat gcagcaaatc
     2641 ttctcgatag ggggatagcg taatgctgat gcagcgatcg gcctgtttta agcatatcag
     2701 ttttttctta attattctgt tcgttagtgt tctgtcagcc gcaggatatg cttcatcctg
     2761 gaacggccat ggccaaaatg acctgtggag caatgcctcc aattggatcg ggggaatccc
     2821 tggtccggca gattacgcaa ggtggaacct cgcaaataca actccgtgtt atctgaatca
     2881 taatgcagga actatcgata ccctgttggt cagcgaatat caacaggggc atttaatcat
     2941 ccaggatggc ggctcactga ccatcaccaa ctatgcctcc attatgaacc gttccggcag
     3001 catcaccgta cagcccaatg gtctgctgca cgtcatagga gatttgataa tcggacaatg
     3061 ggatgatttg ccggatgatg aaatgatctt aacaatgaac ggcgggacaa tagtggtaga
     3121 ggcagtgctt cggtttggtt tttattttga tggtgttaat ggacaatata ccgttaaaac
     3181 tcatgcccag cttaatgggg gagagttata tgccggttcc gtcagcggtt tgcaggcgga
     3241 tcgcggcaca atcgatatta cacacggaaa actaatcctc aatggcaacc ataaggccgc
     3301 cgtacaaaac tgggttacca gcggtttttt aactgcctat gatggtaatg gaatcattac
     3361 atacgactat gaccggacaa atcagggtaa gaccacaatc tgggctgctg ctcccgttaa
     3421 cggcgatgtg gacggcgatt ttgatgtgga ctttgatgat ttgatcttat tcgtcggtga
     3481 ttggctgtcg caggaatgcg gagtccattc tgactttaca ggtgactgca aggtcaatat
     3541 gcaggacctg gcgcagatgt cagagaattg gttttacggt gtaagtcttg ctgcctttga
     3601 tcccaatccg gctgatggtc aggtgattat gccgtcgcag cttttctgga aagcgccgcc
     3661 ctttattgat tcatttgata tatactttgg aatgaattat atggccgtgt taaatgctga
     3721 tcatgaaagt cctgaatttc agggcacaca gtctgaaaaa aactttgccc ctggtttgct
     3781 gtacccggat gagcactatt actggagaat cgatgttgtt cttggggaca agaaataccc
     3841 cggaacagtc tggagtttct cgacagtgga tatggaaaat gccgctggtt ttatcggcgc
     3901 cacctgtggt tggcagtaca gtgcccagct tgaaggaccg ctgacgtatc ccaatcaaac
     3961 caacatttcc ctctataacc cccagcgggg caatccgact gcaacatggg aggactgggc
     4021 ggcccagatt actcaggcgg gactggactt tatctgtccc aatctgaccg gctcctggcc
     4081 caatacgggc tctcctcccg cagatatggc tcctcttgta acggccctca ataacctggg
     4141 caatgttact aaattcgcca tatttgatga caatgccgcc tcctggtgcg ctcaatggaa
     4201 tatggcggag ggacggggat tttggtatgc agaaccgttc gacatcagcg atccggccaa
     4261 ctgggttttc atctatgact ataattacaa actcttctat gaaaccgttc cagatgccca
     4321 gcgattcaag attgacggcc gacctgtcat ctggatctgg acgggcaatc cctatttcgt
     4381 tgccaactca cagggcaacc tctctcgggc tatgctttgg gtacgtcagc ggtgcatgga
     4441 ggattttgga tttaatccct acattgtagg gaatcgagac tttatctcaa acgattccac
     4501 ttgtgccaat ccgggagtgc tggatgccac ccatagttgg tttgggccgc ccaatgaccc
     4561 ttggactttg tccgtcttta atggcgtgaa aatcggtgtg gcggttgcac aatttcaaaa
     4621 ttcaagcaat ccccccggtt ttctggaccc ggatcacggc aatttactga atgaagggct
     4681 tcaccacacg gtcggcgccg gtgccctgat gacgctgtta gaaggattta ccgattacga
     4741 agaagatgcc gctctcttcc gggtgcgcaa cctggatgaa aacggtaatc cgctcagcta
     4801 tgaacagact tattatgact atcccaatca acggctcaac attattcgca aacacagccg
     4861 tcatcccttc cctgcggaac ttaaactgga agcggaagga tgtgatacct acggcggtgc
     4921 ggcaggcggc ggaacaccga gactctatcg caacggcaat atcgggattg aacccacaca
     4981 ggatgaaggg ggcggattca gcatcggctg gattcagccc aatgagtggt ttcagtggga
     5041 agaagtgccg atggaagggt ctggaactct ccaggttcgc gtagccgcaa ataacgatgc
     5101 cggtcggctg cattttgtca tcgatggaca ggtgaaaccc gccgttgcgc tccctgatac
     5161 gggcggattc cagagttggg cgaccgtgga cacaccccct gaaacattcg cgccgggctc
     5221 ttatcatacg gtacgtctaa tttgtgaaac atcaggaatg aacatcaact ggattcgctg
     5281 ggtagccggc ggtacagaca atgctccacc cagagtcgtc attaattccc ccgtaaataa
     5341 cagtacctat gctgagccgg ctgctgtaac catcgaagcg gctgcatttg atgaagatgg
     5401 caccatcagc aaagtggatt tttatcacgg ttcgactttg ctggggacgg ataccgccgc
     5461 tccctattcc tttacatgga ataatgtgcc tgcaggggac tgcggtagtt atcgtctgtc
     5521 ggctaaggca gtggacaatc agggggcagt atccacctct ctgcccgtct ttatgaatgt
     5581 gacacctctg tcgttgaaca aaccggcgta tgcttccttt gttgtaaaca gaaatgttcc
     5641 ctcccgggca gcagacaatg acctaagcac ccgctgggaa tctgcctaca gcgacccgca
     5701 atggatctat attgatttgg gggcggtcta tgacattgga aaaatcgtgc tcaaatggga
     5761 agcggcctat gccagtagct accaaatcca gatatccgac gatgccgtac actggacaac
     5821 cgtgtacagc acgacagtcg gcgacggcgg atatgatgtc ctgaatgtct cggcagccgg
     5881 acgctatgtc aggatgtacg gaacaacccg cggtacaatc tacggatatt cgttgtatga
     5941 ctttaaggtc tatatggctt attaagagtc cagttcagca gagatacggc ggccgcctaa
     6001 aacctataaa aaacagtcaa gttgagaaat attgtttgga tggcgtggat ggcgatatag
     6061 atatggctca atttcacaaa aggaatgctg ttatggatgt atatcagaga acatgcacgc
     6121 aaaccggatt ttttgcttct ttgcaagcgg ccttaaccat ctttctgctt gttgccggta
     6181 ccgcagctgc tgaggtaact ttcagcggtt ccgctccggc gccggattcc gagttggcct
     6241 tgtggtatcg tcaacctgcc gcggattggg aatcccaagc gctgcccatc ggcaatgggt
     6301 atattggagg gatggtattc ggcggtgtgg cgcaggaaca gatcagtctg aatgaaaaga
     6361 ctctctggtc tggtgggccg ggtgaatatc ccgattatac cggcggcaac aatactgacc
     6421 ggacggtcta tttggccaat atccgaaaaa tgctcgaaca gaagaattat gccgccgcag
     6481 atgcgtatgc ggaaaatctc aaaggcatct cca
//
LOCUS       DAOTBO010000130         6386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10303_length_6386_cov_4.054020, whole genome shotgun sequence.
ACCESSION   DAOTBO010000130 DAOTBO010000000
VERSION     DAOTBO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10303_length_6386_cov_4.054020"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..532)
                     /locus_tag="PLE88_11500"
     CDS             complement(20..532)
                     /locus_tag="PLE88_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77130.1"
                     /translation="MNPTTANPCCRRQQRAKNGWFALLRRRLSIRLQDHIASCPRCRR
                     RLADTGKVELALQLLVSEPHCLTLLQRANLAALKMLSHHERYSPQADALRNVLPQPNW
                     TWRHSRRLEKLLQIAACAAVVCLLKLGLFSFFQDIREEGQAAVRNYYASNLPDENLLK
                     ELFDEPSQRA"
     gene            complement(541..1104)
                     /locus_tag="PLE88_11505"
     CDS             complement(541..1104)
                     /locus_tag="PLE88_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOM77131.1"
                     /translation="MDRAAVLHLWAEIGAEAVSASQRWILSAMQQQGPYLVTMLWRIL
                     GNEADVCDAYQETFLNLTYHFEGRRKPRHVRNYLYRVAMNTAISMLRRRRLQQTLQHT
                     LRQHSAGDDSLNYDIDLDSYALEEQLRKAICSLPESLADVVILRDLAELSYAEVAQIL
                     GISAGTARVYRYKAVKALAVLMAEKEQ"
     gene            1285..2454
                     /locus_tag="PLE88_11510"
     CDS             1285..2454
                     /locus_tag="PLE88_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOM77132.1"
                     /translation="MSKEDTKKVVPDVCSLIEHLLSEGIEKGASDIHFDPTAGGLTVK
                     FRLDGSLHIVEHLPGAIQEYVITRLKVLGGLLTYRNDIPQEGHIDWPSGQGAAGTVTD
                     MRLAVFPTVRGQRAVVRLFYDNQDLNNLETLGLPASILQTLKNIASESQGLLLLTGPA
                     GSGKSTTLAAMIGTILQTTPGRSIVTLEDPVERLIEGVSQIQIPPTGELTFSTALRSL
                     LRQDPEVLMLGEIRDAQTAQIAAQAALTGHLLLSTMHSGNCCAALVRLLEMGIEPYQL
                     TSSLLGIVNQRLLRRLCPECRQRGSDGTYQAVGCSCCAETGYRGRILAAEMVEMDSSL
                     RKAVLARADAEEISRLLQGRGCMNLLQNAQRLIEEGLTTAEEVQRVCGRENPASS"
     gene            2467..3663
                     /locus_tag="PLE88_11515"
     CDS             2467..3663
                     /locus_tag="PLE88_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM77133.1"
                     /translation="MALFRYQALTEHGRLMEGTLEAASPTEARAVLEKMNLSVQQIQL
                     CTEPKAPPRIGQNEFLLFNQQLASLTKADIPLEQGLRQLARDAASTRMKQLMEQIADD
                     LQAGLTLEQAVAKREKQFPPLYSLILKTGIQSGRLSEMLIIFNHYLEITQQTRRILIE
                     SLTYPAVVLLLALLIITLVCRFIMPTQEEVIRDFTEGGSIPLITTFYIRMSYHIVEIW
                     VVLLAFAVSGLLVWRFLSSKPAGRLIKESFLFRIPLLGRTLLSSHLARLAESMGLLVA
                     AGCPLPQCFRLGGEASGSETLQRDSHLAAEHIESGGSLLEAAAACRKMPMLFLYSLQL
                     GCQRNDLAGHLKILAQTYVQQTQVLQSYLRAMLDPLAILGLGFIIASMILALFLPYTS
                     IFGIFL"
     gene            3679..5187
                     /locus_tag="PLE88_11520"
     CDS             3679..5187
                     /locus_tag="PLE88_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM77134.1"
                     /translation="MNLLSVFLIDERISVPFLLIFGAALAVLLLWLKRRSLSAAFGAT
                     CLFPLVYCTAGLLLSYQSYFHIGPYPMVGIMGVCLSVLMFLWGLFSISRVPADWKGER
                     LAWPIIWARWLLIIILALNFLFLLLWGFQVVGILLFCLFTGAVWQAVQAARFARSFEI
                     ISTLTLCVRQNLPLAMAIKTAAQHSKKSVKSILERITHWLVQGYPLSEALRKGWPQAP
                     AHLTAALYAGEQIGQLPQTLETLLADQSEKAWTKQYQSNWLIYPAAVLVLIILITSGL
                     LTFVFPTFLAIITDVEMGRAQLPLATQVILAIFQILLHSLKFFLILVTAAVVYAAVAA
                     FYSHRGRPLRLKERIVWHLPLLGQFHQQRSLRQLLQYLRPALEVGLDLPEALEKALSL
                     KLNPCFQKQIQRWLHYLRRGYSPDAAALLAKMDSKIVWAFSRPNQSCLPGVLSMLEES
                     IGHTLHYRRRLLSSIAFPLTILVMAVVVGTVVLAFFLPVVHIIYITGGLVLP"
     gene            5201..5587
                     /locus_tag="PLE88_11525"
     CDS             5201..5587
                     /locus_tag="PLE88_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77135.1"
                     /translation="MKCMIQKRCGFLTIEMFVTLALLMMLFVLLTDTLLQTGRLNRTQ
                     WAEQMCLAAVQAQLDSLTARGKPLDPADCRRLWPAVDMTVQLSPGEGPWAGLTKADVQ
                     AEQRIFRKTIRIRQSRYLLVPQEVSQ"
     gene            5584..6090
                     /locus_tag="PLE88_11530"
     CDS             5584..6090
                     /locus_tag="PLE88_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77136.1"
                     /translation="MKRLRKAYSLNELIFIMALLTVIMSFSSKIFYSLVKEIPLMIRS
                     VNVQSCTDAMLESLAEDVEKARRIQLSVVPENQLTLEGPQGVVFYTFHQEQMRRTLPD
                     PSQTGHSVWPLPDVRLEWQLYKDNDCPAALEVCTWQQRILLGQKQIHFRQKRLFFVGL
                     QKESDIDE"
     gene            6083..>6386
                     /locus_tag="PLE88_11535"
     CDS             6083..>6386
                     /locus_tag="PLE88_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77137.1"
                     /translation="MNKKQNKSGFILLAAIAVIPLVGIGISLLSRFGHQMVLQTRWRT
                     LQGQARNLAFSGRSWAELHKTQLLSAPPQQTWMLKIDSLQAADGECRIQRLSDDSEG"
BASE COUNT         1368 a         1755 c         1684 g         1579 t
ORIGIN      
        1 gcggttcgga cagaaaatat taagcccgct gagaaggttc gtcgaacaac tctttgagca
       61 ggttctcatc cggaagattg gaggcataat aattccgcac cgccgcctgt ccttcttcgc
      121 ggatatcctg aaagaatgaa aacaggccca gtttcagcag acaaaccacg gctgcacacg
      181 ctgctatctg aagcagtttt tccaaacgtc tgctgtgccg ccaagtccaa ttgggctgcg
      241 ggagaacgtt gcgtaaagca tcggcctggg ggctgtaccg ctcatgatgg gacagcatct
      301 tcagggcggc cagattggct cgctgaagca gggtcagaca gtgaggttct gagacaagca
      361 gctggagggc cagttccacc tttcctgtat ccgccagccg gcggcggcac cgcggacagg
      421 aagcaatatg gtcctgaaga cgaatggaaa ggcggcggcg aaggagagca aaccagccat
      481 tttttgctct ttgctgccgt ctgcaacatg gatttgctgt tgttgggttc atggctgcac
      541 ctattgctct ttctcggcca ttagcacggc caatgctttg acagctttat aacgatagac
      601 ccgagccgtc cctgcgctga tgcccagtat ctgggcgact tccgcataag acagctcggc
      661 caaatcccgc agaatcacta catccgccag ggactccggc agagaacaga ttgctttgcg
      721 aagctgttcc tccagggcat aactgtccag gtcgatatca tagttcaggg agtcatctcc
      781 agcagaatgc tgacgaaggg tatgctgaag tgtctgctgg aggcggcgtc ggcgaagcat
      841 ggaaatcgcc gtattcatcg ctacgcgata aaggtaattc cgcacatgcc ttggcttccg
      901 acggccttca aaatgatacg tcaaattcag aaaggtttcc tggtaggcat cgcagacgtc
      961 cgcctcgttg ccgaggattc gccagagcat tgtcaccaga tagggcccct gctgctgcat
     1021 ggcagacaaa atccaccgct gcgaagccga aacggcctct gcccctattt cagcccagag
     1081 atgaagaacc gccgctctgt ccatacacct gccttatctg tatatcagga cgcggtacat
     1141 gcctctatgt tatctttttt tctgcataaa aggggaaaaa ttctggaaaa attttttgat
     1201 taacattttt cgtccccagc cgtcctaatg gacagaacag gaaccagtcc caggaactat
     1261 cggcagcaaa aaacttatcc tgttatgagc aaagaagata caaaaaaagt tgttccggat
     1321 gtctgctcgc tgattgagca tttgctctcc gaaggaatcg aaaaaggagc cagcgacatc
     1381 cactttgacc cgaccgccgg cggtctgacg gttaagttcc gtttggacgg cagtttacat
     1441 atagttgagc atctgccggg cgctatccag gaatacgtca ttacgcgatt aaaagtcctt
     1501 gggggtctgc tgacctaccg caatgatatt ccgcaggaag gccatatcga ttggccgagc
     1561 ggccaagggg cagccggcac agtgactgat atgcggctgg cggtttttcc aacggttcgc
     1621 ggccaaagag ccgtcgtacg gctgttttat gacaatcaag accttaataa ccttgaaacg
     1681 ctcggtcttc cggcgtctat tcttcaaacc ctcaaaaaca tcgcttccga atcgcaaggg
     1741 ctgcttttgc tcaccggccc tgcgggcagc ggaaagagca ccactctggc cgccatgatt
     1801 ggcaccattc ttcaaacaac gcccgggcga agcattgtta ccctcgaaga tccggtggag
     1861 cgattgattg aaggagtcag ccagattcag attcctccaa cgggggaact tactttttca
     1921 actgcgctgc gttctttgct cagacaagac ccggaagtcc tgatgctggg ggaaatccgc
     1981 gacgcgcaaa cagcccagat tgccgcccag gccgccctca ccggacatct gctgctgagc
     2041 accatgcaca gcggcaattg ctgcgcagcc cttgtccggt tgctcgagat gggcattgaa
     2101 ccctaccagc tgacctcttc gctgctcggc attgtcaatc agcggcttct gcgtcggctt
     2161 tgtccggagt gccggcagcg gggttccgat ggaacatacc aggccgtcgg gtgcagctgc
     2221 tgcgccgaga cgggataccg gggacggatt ctggctgccg aaatggtcga aatggacagt
     2281 tcgctgcgaa aggccgtgct tgcccgtgcc gacgctgaag aaatctcccg acttctccag
     2341 ggccgcggct gtatgaacct tctccaaaat gctcaacgtt taattgaaga ggggttgaca
     2401 accgctgagg aggttcaacg tgtttgcggc agggaaaatc cagcttcttc ttgagaggaa
     2461 aatcctatgg ccctctttcg ctaccaggca cttacagaac atggacgctt gatggaagga
     2521 actcttgaag cggccagccc cactgaagcc cgggcggtac ttgagaaaat gaatctttcc
     2581 gttcagcaga ttcagctgtg cacggagccc aaagcccctc ctcgcatcgg acaaaatgaa
     2641 tttctgctgt tcaaccagca gctggcttct cttaccaaag ccgacatccc gcttgagcag
     2701 gggcttcgcc aactggctcg ggacgccgcc tcgaccagaa tgaagcaatt gatggagcag
     2761 attgctgatg atcttcaggc gggtctgacc cttgaacagg cggtcgccaa gcgggaaaaa
     2821 caatttccgc cgctctacag tctcattctt aaaaccggca ttcaatcggg acgactgagt
     2881 gaaatgctga ttatcttcaa ccattatctt gagattaccc agcaaacacg gcgtattctg
     2941 attgaatccc tcacctatcc ggctgttgtt ttactcctcg cccttctgat catcacgctg
     3001 gtgtgccgat tcattatgcc aacccaagaa gaagtcatcc gcgatttcac agagggcgga
     3061 agcatccccc ttatcaccac cttctatatc aggatgtctt accatatcgt tgagatttgg
     3121 gttgtcctgc ttgcgtttgc ggtgagcgga ttgttagttt ggcggtttct ttcgtccaag
     3181 ccggccggcc ggcttatcaa agagagtttt ctttttcgga taccgctgct gggccgtacc
     3241 ctgcttagca gccatctggc ccgtctggct gaatccatgg gtctgctggt ggctgccggc
     3301 tgccccctgc cccaatgctt tcgactcggc ggggaggcca gcggcagcga gactctccag
     3361 cgagacagcc atcttgcggc tgaacatatt gaaagcggcg ggtcgctcct tgaggcggcg
     3421 gcggcctgtc gaaagatgcc gatgctgttc ctttattcac tgcagttggg ctgccagcgg
     3481 aacgacttgg ccggccactt gaagattctt gcccagacct atgtgcagca gactcaagtg
     3541 ctgcaatcgt atcttcgggc aatgcttgac cctttggcta ttctcggatt agggttcatt
     3601 atcgcaagca tgattttggc tctgtttctg ccctatacca gtattttcgg aatttttttg
     3661 taacaaacgg gagacgatat gaacttgctc agcgtcttct tgatcgacga acgcatctcc
     3721 gtcccgtttc tgctgatttt cggagctgca ttggccgtgc tgctcctttg gctgaagaga
     3781 cggagtctgt cagcagcttt tggagccact tgtctttttc cgttggttta ttgtaccgcc
     3841 ggcctgctgc tgtcctacca gtcctacttc cacattggtc catatccgat ggtgggcata
     3901 atgggtgttt gcctatccgt tctgatgttc ttgtggggac tgttttctat ctcccgcgtg
     3961 ccggccgact ggaaaggaga gcggcttgcc tggcccataa tctgggctcg ctggctgctc
     4021 ataatcatcc tggcccttaa ctttttgttt ctgctgctgt ggggctttca ggtcgtcggg
     4081 atacttttgt tttgtctgtt tacaggggct gtctggcagg ccgttcaggc cgcccgcttc
     4141 gctcgttcgt tcgaaattat ttcgacgctg accctctgcg tccgccagaa tcttcctctg
     4201 gcgatggcaa ttaagaccgc cgcccagcac tccaaaaaat ctgttaaatc tatcctcgaa
     4261 cgaataaccc attggcttgt gcagggctat cctttgtctg aagccctccg aaaaggatgg
     4321 ccgcaagctc cggcgcacct gacggcagcg ctctatgccg gcgaacaaat cgggcagctc
     4381 ccccagaccc tcgaaaccct tttggctgac cagagcgaaa aggcatggac taaacaatac
     4441 cagagcaact ggctgattta tccggctgct gttttggtcc tgattattct gataacttct
     4501 ggactactta cttttgtgtt ccccacgttt cttgcgatca tcaccgatgt cgagatgggc
     4561 cgggctcaac tgcctttggc aacacaggtt atactggcta tttttcagat tcttttgcat
     4621 tctttgaaat tcttcctgat tttggtgaca gcggcggttg tgtatgcagc cgtcgccgcc
     4681 ttttattccc accgaggccg gcccctccgg ctcaaggagc gaatcgtctg gcatttgcct
     4741 ctgttgggcc aatttcatca gcagcggtcg ctgcggcagc ttctccagta tctccggccc
     4801 gctcttgagg ttggtttgga cctgcctgag gccctcgaaa aagccctttc tttgaaactc
     4861 aatccctgct tccagaagca aatccagcgc tggctccact atttgcgccg cgggtactcg
     4921 ccggatgctg ctgcgctgct ggccaagatg gattccaaaa tcgtgtgggc attcagccgg
     4981 cctaatcaga gctgtcttcc gggggtgctg tcgatgctgg aagaatctat cgggcatacg
     5041 cttcactatc gccgtcggct gctttcctcc atcgcttttc ccctgaccat tctggttatg
     5101 gcggtcgtgg taggaaccgt tgttctggct ttctttttac cggtggttca tatcatttac
     5161 atcactggag gtcttgttct cccttgaagg gaacacgaag atgaaatgta tgattcagaa
     5221 aagatgcggt tttttaacca ttgaaatgtt tgttacgctc gcgctgctga tgatgctgtt
     5281 tgtgctgctg acggacacac tccttcaaac cggccggctc aaccgcactc aatgggcaga
     5341 gcaaatgtgt ctggcggccg ttcaagccca gctggacagt ctgacggcca gaggcaaacc
     5401 cctcgatccg gcggattgcc ggcggctttg gccggccgtc gacatgaccg ttcaattatc
     5461 gcccggagaa ggcccctggg ccggcctgac caaagccgac gttcaggccg agcagagaat
     5521 ctttcgcaag accattcgca ttcggcaaag ccgctatctg cttgtgccgc aggaggtctc
     5581 ccaatgaaac gccttcgaaa agcgtattcg ctcaatgaac tcatcttcat catggccctg
     5641 cttacggtga ttatgagctt cagcagcaag attttttact ctttggtcaa agagatcccg
     5701 ctgatgattc gctctgtcaa tgttcagagc tgtacggatg cgatgcttga gtcgcttgcg
     5761 gaggacgtag agaaggcgcg gcgaatccag ctttctgtgg tgccggaaaa tcaactgacc
     5821 ctcgaaggtc cgcaaggggt tgtcttctac accttccatc aggagcagat gcggcgaacc
     5881 ttgccggacc ccagtcagac aggccattcc gtctggcccc tgcctgatgt ccggctggaa
     5941 tggcaactgt ataaggataa tgactgtccc gctgctttgg aggtgtgcac atggcagcag
     6001 cggattcttt taggccaaaa gcaaattcat ttccgccaaa aacggctgtt ttttgtcggt
     6061 cttcagaagg aaagcgatat tgatgaataa aaaacaaaac aaatcaggat ttattttgct
     6121 ggcagccatc gcagtgattc cgcttgtcgg catcggcatc agcctgctca gccgcttcgg
     6181 tcatcagatg gttctgcaaa cgcgctggcg aactcttcag gggcaggccc gcaatctggc
     6241 cttcagcgga cgctcgtggg cggagctcca caaaactcag cttttgtccg ccccgccgca
     6301 gcagacgtgg atgttaaaaa tcgactctct acaggcagct gacggagaat gccgaatcca
     6361 gcggcttagc gatgattcgg aaggga
//
LOCUS       DAOTBO010000131         6334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10407_length_6334_cov_5.159261, whole genome shotgun sequence.
ACCESSION   DAOTBO010000131 DAOTBO010000000
VERSION     DAOTBO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10407_length_6334_cov_5.159261"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLE88_11540"
     CDS             complement(<1..881)
                     /locus_tag="PLE88_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77138.1"
                     /translation="MKQIATTAAYLLTALIVQAHLVDDFESYQTGGIGTVTTAWVGSS
                     DGGVNPSTASIITDTIDLRNKAIQLTEGGGTGQQWVRCALSAPAQIAQAGTGTLFVRF
                     RAAAASDISFGLTDLDTASNDWNHFRVQIAVINGTIRARDGGSTRILAWKRNGTQVAL
                     NTDWYYLWVVVDNNADNIRVYLNQTGADATENDRLVRSDLLTQETFAFRSAADTLDQF
                     FWRAQNGAVSRLLVLDDIHIRQGADLTVPAMNKPYSPAVAQTQDNANNDVDVVLSWKA
                     AADPTGVNAVNPDIVDQ"
     gene            complement(938..3241)
                     /locus_tag="PLE88_11545"
     CDS             complement(938..3241)
                     /locus_tag="PLE88_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77139.1"
                     /translation="MKKSVCTLFFLLLVYAAQGVLLDDFESYTPGALVNAGGTPWTML
                     PSWSGAAYFQAEADNTYVAYWGANNGTRGIYRSLGDNRIPASDTATTVFMRVLAETAN
                     TDASFGLTDLAVPTLGGDGYPVFGDFEVQAALISGTLRARNAGTIVNLATISVGTWYN
                     IWLVVNNSTDTYSLYMTTGQTSAMGTTPLASNFGFRNGAAANDLVTILAIGATKANPE
                     RVRIDDIHIMPGVNLTNPTAGPYDPVVNQITPTAIVLQWKAAPDPAGVYAVNPAIVDQ
                     YVFMSTGNPADPNLYYIGATGADPGTDNPLSQYPASGTITVAYDKTYYWAVVEAIEGY
                     QQSFSPGASFKSVDPNNIIGGIWTYVSTKSQPIITVQPADVRVFTTDSAASFTVEFTS
                     PVNPVTAAWYKNDVPLTAGGDISIVTDPHASSTLTIATPELADEGKYYCILSVQEGTA
                     DDLQTATRMLVIKKELARFEFEENLADSSGNNAPAGLAKSVAHLAEPNDAAAVIIEPV
                     YVDGIDGKAVYLSSRQYIDFGIDGYPKAGPLNTLGDIRGASYEKVGFGRGMDQGSILC
                     WVKPQSNAVIYSNANAGDATHFALTTNGTTTARGIVRGNNYDDTAQNLGEASGSLQMT
                     GFNLQDGKWHMFAATWQDSTIRVYLDGEQVATNTQGYTEKYLPWQRGNLLGASRTAAN
                     RAILNAADFLTGAVDKLRVYNYVIWPDEIAVEYQTLSGRTPCANHSFDGYLYNLDNTE
                     LSYCRIDLADFAVMASNWLAAGLYAGL"
     gene            complement(3498..4259)
                     /locus_tag="PLE88_11550"
     CDS             complement(3498..4259)
                     /locus_tag="PLE88_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77140.1"
                     /translation="MKKMLLLCSVTVFMSQAGAVLVDNFESYQAGLIRDGVTGGVWKE
                     ITAGTSFAVIGEDAGNKFLQTGWSGGGRGAYRCIDPIVDSVSAITLYLRIYAVTSSQD
                     TSFGLADQITTSAATWGDFEVQMVLGNGDDADHVNLRARDGGTVETYMALAVGQWYNI
                     WAVIDQTTDSFDMYVTAGYESAVGLSPINPDPINFRNGTTADLSYFLALTNYRDFNYR
                     LDDIHITLGKNLAIPEPVTMLMMGLGGLMLALRKR"
     gene            complement(4373..6085)
                     /locus_tag="PLE88_11555"
     CDS             complement(4373..6085)
                     /locus_tag="PLE88_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM77141.1"
                     /translation="MNVDKESFELNRQILSVIDGTISQEEFSALKQMIRENPKVTGHY
                     IQFMMLYACLRQPGQISTHFVQEAGTKDPVLDEKIWKELAYFEHTAQTLEVPKPSRTA
                     SEAIQPKAAPVRIQHHVSKIPWIVSFLSLAASLAMIVYVIYFYPQTSTPVVGVLTGQI
                     GAQWGQTGGPLEPGGDLHAGPMHLTRGLVSLRLDSGAEVVVEAPAELELLTTNGLSLS
                     HGKLVARVGRDAVGFTVHTPQSKVLDMGTEFGVSVEESGIEQIHVFKGEIALYPNAGT
                     EHFSVSSGQGRIVDSAGRISTTKANPFYFTQPDEFAWQLLAQKGDSYHRWKAFVSELQ
                     RDSSLIAHYFYEMQSDQPDCVMNFAAATYGKLNGQLGDEGRTKPQWVQGRWPQKQGIR
                     FERNKQQVVIIPADPLLAVTEPLTISTWVCFPNADQYGGHLVSCRDNYRVNYQFSIFD
                     HNYQFDYQINRFEFIQYSQRDDKKLYSEKFIPKPQQWYHFAVTYRNAAVSFYVNGTLF
                     ESQPYPASVKPVPAPLIVGTMRTRDYAYEKGDFDGIIDELMIFSRALTAEEIRRIYEA
                     GRPD"
     gene            complement(6082..>6334)
                     /locus_tag="PLE88_11560"
     CDS             complement(6082..>6334)
                     /locus_tag="PLE88_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOM77142.1"
                     /translation="LQQTERISIHNTDYNAALQHCMEKLNPQDRTVIRLRYEHEMSTR
                     MLALQLGKTLENTYKILARIHTLLQRCVQKTLLVWDTHP"
BASE COUNT         1558 a         1690 c         1483 g         1603 t
ORIGIN      
        1 tattgatcca caatatcggg attcacggca ttgacgccgg ttgggtccgc agcagccttc
       61 caggacagca ccacatccac atcattgttg gcattgtcct gcgtttgtgc tacagctgga
      121 ctatagggtt tgttcatcgc cggcacagtc aaatcagcac cctgccgaat atgaatatca
      181 tccagcacca gcagacggct tactgcacca ttctgggcac gccagaaaaa ctgatccagc
      241 gtatcggcag ccgagcgaaa cgcaaatgtc tcctgcgtca gaaggtcgga acgcacaagc
      301 cggtcatttt ccgtagcatc tgcaccggtc tgattgagat aaaccctgat attatcagca
      361 ttgttgtcta caaccaccca cagataatac caatccgtat tgagtgccac ctgcgtgccg
      421 tttcgcttcc acgccagtat gcgtgtgctg ccgccgtcgc gggcacggat tgttccgtta
      481 ataaccgcta tctgcacacg aaagtgattc cagtcgttgg aagcggtatc caaatcggtc
      541 agaccaaatg aaatatcgct cgcagccgct gcccgaaacc gcacaaacaa ggtcccagtg
      601 cctgcctggg ctatttgggc aggggcactt aaggcgcatc gtacccactg ctgtcctgtg
      661 ccgccgcctt cggtcagctg tatcgcttta ttgcgcaggt caatggtatc tgtgattatt
      721 gacgccgtag aaggattgac gccgccgtca gaactgccga cccaagcagt cgttacagtt
      781 ccaattccgc ccgtctgata gctttcaaaa tcatccacca gatgggcttg gacaatcaga
      841 gctgtcagca gatatgcggc tgtagttgct atctgtttca tattgttttc cctttcgttc
      901 tataaggttt cattgctcac attctcatct gtccggttta taatcctgca tacaggccgg
      961 ctgcaagcca gttggacgcc atcactgcaa agtcggccag atcgattctg cagtaagaca
     1021 attcggtgtt atcgagattg tagagatagc cgtcgaaact gtggttggca cacggtgttc
     1081 ttccgctgag cgtctggtat tcgacagcga tttcatccgg ccagataaca taattataca
     1141 cccgcagttt atccaccgcc cctgtcagaa aatcagccgc attcaggata gcacggttgg
     1201 cggccgtgcg ggatgccccc agcaggttcc cccgctgcca gggcagatac ttttccgtat
     1261 aaccctgagt attggttgcg acctgttcac catccagata aacacgaatg gtgctatcct
     1321 gccacgtagc tgcaaacata tgccacttgc cgtcctgcag attaaatccg gtcatttgaa
     1381 gactgccgct ggcttcaccc agattctggg cggtatcgtc ataattgttg ccgcggacaa
     1441 tccctcgtgc agtagtcgtg ccattggtcg tcagagcaaa atgggtggcg tcccctgcat
     1501 tggcattgct gtaaataacc gcattggact gcggcttgac ccagcagaga atcgagccct
     1561 gatccattcc gcggccaaag ccgacctttt cataggatgc cccccgaata tcgccaagcg
     1621 tgttcagcgg cccggctttg ggatagccgt cgatgccaaa gtcaatatac tgccggctgc
     1681 tcagatacac tgctttgccg tcaatcccat ccacatagac aggctctata ataacggcag
     1741 ccgcatcatt cggctcggcc agatgagcca ccgatttggc cagaccagcc ggtgcattgt
     1801 tgccgctgct gtcggccaga ttttcttcaa attcaaaccg tgccagctct tttttgatca
     1861 ccagcatgcg ggttgcggtt tgcaaatcat ctgctgtccc ttcctgaacg ctcaggatgc
     1921 agtagtactt gccttcatcg gccagttcgg gagtagcaat agtcaacgtg gatgaagcat
     1981 gaggatctgt aacaatggaa atatcgccgc cggcagtcaa gggcacatca tttttatacc
     2041 aagcggctgt taccggatta acaggacttg taaactccac tgtaaatgag gcagcagaat
     2101 cggttgtaaa cacccgcaca tccgccggct gaacagtaat aatcggctgt gactttgttg
     2161 acacatatgt ccagatgccg ccgatgatat tgttgggatc cactgacttg aatgaagcgc
     2221 cggggctaaa cgactgctga taaccctcta tagcttccac aactgcccag tagtatgtct
     2281 tgtcataagc aacggttatt gtcccagatg cagggtattg agacagcgga ttgtctgttc
     2341 ccgggtccgc gccggtagcc ccaatataat acagatttgg atcagccgga tttcccgttg
     2401 acataaagac atattgatcc acaatagcag gattaacggc atagacgccg gcaggatccg
     2461 gagccgcttt ccactgcaat acaatcgccg tcggcgttat ttgattgacg accggatcat
     2521 acggtccggc tgtcggattt gtcagattga cacccggcat tatatgaata tcatcaatgc
     2581 gaactctttc cggattggct tttgttgcgc cgatcgcaag aatagttacc agatcgttgg
     2641 cagcagcgcc attgcggaaa ccgaaattag acgccagcgg agtggtgccc atagcactgg
     2701 tctgaccggt ggtcatataa agactgtatg tgtcggtgct gttattgaca accagccaga
     2761 tattatacca agtaccgacc gaaatagtcg caaggtttac aatcgttcct gcattgcgtg
     2821 ctctgagcgt accgctgatt agggctgcct gcacttcaaa atcaccgaat acaggatagc
     2881 catcgccgcc gagggtaggt acagccagat ctgttaaccc aaatgaggca tcggtattgg
     2941 ctgtttcggc taaaaccctc ataaagactg ttgttgcagt gtccgaagcc gggatgcggt
     3001 tatctccaag cgaacggtag atgccccggg ttccattgtt tgcaccccaa taagcaacat
     3061 aggtattgtc tgcttccgcc tgaaaatagg cagcgccaga ccagcttgga agcattgtcc
     3121 aaggcgtacc gccggcatta accaatgcac cgggggtata actttcaaaa tcatccagca
     3181 gaactccctg tgccgcatac accagcagca gaaagaacag tgtacataca gattttttca
     3241 taattgtatc ctttcgctta aaaattatta attcacacac tgaatacggg ttgccccata
     3301 aattatgtta tttcatcggt tcttatgcca tactctcttg acttgctgtt ttgcctctgc
     3361 tgcattgtca acatatacaa aacagtcctc ataaaaaggc cgtcattaat cctgtcatca
     3421 cagtcctaaa attgctatcc tttttggatg cagaattgtc atggcaattt ctgtgcggat
     3481 agccggcatg taaggcctca ccgcttccgc agtgcaagca tcaggccgcc cagtcccatc
     3541 atcagcatcg tgaccggctc aggaatagcc agattctttc ccaatgtgat gtggatatca
     3601 tccagccggt aattaaaatc ccggtagttg gttagggcca gaaagtacga caaatcagcc
     3661 gtcgttccat tgcggaagtt aatcggatcg ggattaattg ggctcaagcc aacagcactt
     3721 tcataaccgg ccgttacata catatcgaac gaatctgttg tctggtcaat aacagcccag
     3781 atgttgtacc actggccgac agccaatgcc atataggtct caaccgtgcc gccgtcgcgg
     3841 gcacgcagat ttacatggtc ggcatcatcg ccgttgccaa gaaccatttg aacttcaaaa
     3901 tctccccatg tagccgccga tgtcgtaatc tggtcagcca gaccgaaact ggtgtcctgc
     3961 gagctggtaa cggcataaat ccgcaaatac agtgtgattg cggatacaga gtctacaata
     4021 ggatcaatac agcggtaggc tccgcggccg cctccgctcc agcccgtctg gaggaacttg
     4081 tttcccgcat cctcgccaat gacagcaaag gatgtaccgg cggtaatctc tttccagaca
     4141 ccgcctgtaa caccatctct gattagtcct gcctgatagc tttcgaaatt gtctaccaga
     4201 acagccccgg cttgggacat aaaaactgtc acactgcaca ataagagcat ctttttcatc
     4261 tttcaatcct ccgcatatgc ctgctggctc attcagaaaa cggtcttttt gagcaggcgg
     4321 ccaaaaccgt tttaccatta ttttctaacc ttcagcggca agtcggcatg ctttaatccg
     4381 gcctgcccgc ttcataaatc cgtcggattt cttctgccgt cagggctctg ctgaaaatca
     4441 tcaactcatc aataattcca tcaaaatctc ccttttcata ggcgtaatct cgggttctca
     4501 tcgtgcctac aatcagcgga gccggcacag gcttgaccga tgccggatac ggctgagact
     4561 caaacagtgt tccattgaca taaaaagaaa ctgccgcatt gcgataggta acagcaaagt
     4621 gataccactg ttgcggcttg ggaataaact tctccgaata caatttttta tcgtctcttt
     4681 ggctgtattg gataaactca aacctgttta tctggtaatc aaattggtaa ttgtggtcaa
     4741 aaattgagaa ttggtaatta acccggtaat tatcccggca ggaaacaaga tggccgccat
     4801 actggtctgc attggggaag caaacccatg tagaaatcgt taagggttcg gtaacagcca
     4861 gaagaggatc agccggtata atcactacct gctgcttatt ccgttcgaag cggataccct
     4921 gcttttgggg ccagcgtccc tgcacccact ggggtttggt gcgtccttca tcacccagct
     4981 gcccattcag tttcccatat gttgcggcgg caaagttcat cacacagtcg ggctggtcag
     5041 actgcatttc gtaaaaataa tgagcaatta aagaggagtc ccgctgaagt tcggagacga
     5101 aggctttcca tcgatggtag gaatcccctt tttgtgcaag cagctgccaa gcaaattcat
     5161 cgggttgtgt gaaatagaag ggattggcct tcgttgtcga aattcgcccc gcactgtcca
     5221 ctattcgccc ttgcccgctt gaaacagaaa aatgttctgt gccggcattg ggataaagtg
     5281 cgatttctcc cttaaaaacg tggatctgct cgatgcccga ctcctcgaca gagacaccaa
     5341 attccgtccc catatccagc actttactct gcggggtatg gacagtaaaa cccactgcat
     5401 cccttcctac tctggcaact aactttccat gtgacagtga aagcccattg gtagtcagca
     5461 attcgagttc tgcaggggcc tcaaccacca cttccgcccc gctgtcgagc cgcagggaga
     5521 ccaatcccct tgtaagatgc atcggcccgg cgtgcaaatc cccgccgggt tccaacggac
     5581 cgccggtctg cccccactga gccccaatct gccccgtcag cactccgacc acaggagtgc
     5641 ttgtctgggg ataaaaatag ataacataaa caatcatggc cagtgacgcg gccaatgaca
     5701 aaaatgaaac aatccaaggg attttcgaaa catgatgctg tattcttacc ggagcagctt
     5761 tcggctgaat agcctccgag gctgttcgtg agggcttggg gacttcgagc gtctgtgctg
     5821 tatgctcaaa ataagccagt tccttccata ttttttcatc cagcacagga tctttcgttc
     5881 cggcttcctg cacaaaatga gtacttatct gaccgggctg tctgagacag gcataaagca
     5941 tcataaactg aatatagtgc cctgtgacct tcggattttc ccgtatcatt tgcttcagcg
     6001 cagaaaactc ctcctgactg atggtcccgt caatcacaga aagaatctgc cgattcaatt
     6061 cgaatgattc cttatcgacg ttcatggatg ggtatcccag acaagcagcg ttttttgtac
     6121 acagcgctgc aggagtgtat gaatgcgggc aagtatttta taggtattct cgagtgtttt
     6181 tcccaattgg agtgccagca ttcgggtact catttcgtgc tcataacgaa ggcgaataac
     6241 ggtgcggtct tgcggattca gtttttccat acagtgctga agtgctgcat tataatctgt
     6301 attatgaatg cttatccttt ctgtctgctg aagg
//
LOCUS       DAOTBO010000132         6302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10471_length_6302_cov_6.185049, whole genome shotgun sequence.
ACCESSION   DAOTBO010000132 DAOTBO010000000
VERSION     DAOTBO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10471_length_6302_cov_6.185049"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="PLE88_11565"
     CDS             complement(<1..1588)
                     /locus_tag="PLE88_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOM77143.1"
                     /translation="MNDYSVSPSGEKFPLPKPEEYPAEFKRLETLVSTARQEGKEIVV
                     VMGVGFVGAVMAAIIADTVDKKTGKPSKFVIGCQRPSTRSYWKIPMLNRGQSPVKAED
                     PQVDPMIARCVKDKKTLVATYNSDCLSLADCVVVDVQCDFSKRSLGNLRTGEAEMKAL
                     EATLKTIGEKVQPNCLTLIETTVAPGTTEFVAWPILKKAFKARGIASEPLLAHSFERV
                     MPGREYVSSIRDFWRVCSGCNEAAKIRVEKFLREVLNTEKYPLTVMDRPIESETTKIV
                     ENSYRAVILAFLDEWSLFAERNGVDLIKVIKAIKMRPTHSNIIFPGPGIGGYCLPKDG
                     GLGYWAYRHILGFNDDIFKLTTAAIDINDTRGLHAATLTRDALRNMGRYIAGASVLLC
                     GASYRQDVADTRYSGSEIVVRKLTEMGADVKVHDPYVEHWYELEKQDEYPAPGHSWAR
                     FFRNQDDLVNLRVEKDLPAALKGAEAVILAVPHAPYLNLEPDQIVNWVGGPLAVIDCY
                     GILDDAKIRRYFELGCEVKAL"
     gene            1780..2997
                     /locus_tag="PLE88_11570"
     CDS             1780..2997
                     /locus_tag="PLE88_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOM77144.1"
                     /translation="MIDNQTRVCVLTGRKMAAIASIALEGPESQTILGKIFRKNPFNK
                     HRSNTAADTGVIDLQQVLYGCIMDGSQVIDEAVVGCEEKDSYVIHCHGNPLLVERIIN
                     LLQSYGAQLTEAETFFLRRYRRQSGSLIEAEAKLAMQTSYTIAGVRILQAQIAAGLTL
                     WAGRMMDAVNILTREQIQNECRQILANSIIARRIIHGVRIVIAGPPNSGKSTLLNCLA
                     GRQEAIVSDIAGTTRDWISIVCRMEPLYAEFIDTAGLDTSLAQDYALQRHAQQITQQL
                     LENCDLILHLHDAAALEDSGIQSINTNVIANFAQKKPIINIYNKIDLLKDRNIPHCLP
                     NGLCISAKEGIGIELLGRTIGKTLGTNDFDANQPVVFTDRQRAIISQIMNSAESLRDL
                     LSDLIGLSYPSTH"
     gene            3195..4487
                     /locus_tag="PLE88_11575"
     CDS             3195..4487
                     /locus_tag="PLE88_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM77145.1"
                     /translation="MAQEQQDKKDTPAADAMQKQIALQEAQFYAKGRLMNNMAYQIRT
                     LSNAIVGFSDLLLSEPLSPDQLEYVREINQAGNGLSALVNEVLDWTQVMTGRIAVSKT
                     SCDLAELLGRLEAILRSAAREKGLACAVQLDPQLPDSIVCDSEHLYKCLLNLLSNAVK
                     YTRKGSIRIHVRLDDSQAEPAVCFDVIDTGIGIEADKIDSIFDPAQKREESNEQVQTL
                     FNLGLTVTAGLSLTKQLCHLIGGTIEVTSQVNSGSTFTLRIPAGLELSTRQESPIYSH
                     PKQETQKSASEPALILLAEDQPSNRTVITLMLEAMGVRVDTAQDGREAVQKALQNTYD
                     LILMDLKMPTMDGYEAAAQIRRQNHVPMVALSAKVFDEDEHRRITALFEGFLTKPVDG
                     RKLAQTLEQFIDSFQYSAPTAAASAGRPAENIAYEYRT"
     gene            4500..6281
                     /locus_tag="PLE88_11580"
     CDS             4500..6281
                     /locus_tag="PLE88_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOM77146.1"
                     /translation="MTANVSRQVQYSGMPLAVKENQMFGDWLVQNGYITEQQLAAALN
                     DQKTSGGRLGQNLVRLAYLNDEQLTQCLAAYLNLESISLSDLSVINKEVARRIPENIA
                     KRFHSLAVDAKDDRILVAMADPLNVIAVDTVSVKLKKNIQVVLASEREIQRAIEYVYH
                     GTDVEEQQLRDLVELEISSDEDDLMENTLAMEVSAAAEAQDAPVIRFVDLLLSQAIKS
                     RASDIHIEPQDKSMSIRMRIDGLLTEMVPPPRKMQPAVIARIKILSEMDIAERRLPQD
                     GRFKIRRGQGKEEIDVRVSSLPTIYGEKIVMRILDKGAVTHNLDSLGLEPDLLAKFKM
                     TLQQPHGIIIVTGPTGSGKSTTLYSALNELRDPHKNITTVEDPVEYRLEGINQTQIKP
                     SIGLTFASCLRTILRQDPDIILIGEIRDKETMEIAIKASLTGHLVLSTFHTNDAPSAL
                     SRLVYMGLEPYLLASSLNLILAQRLVRRICDKCKAPQTLTETQIKRLELTPEQIANTT
                     FYHGKGCSECGGSGYRGRLPIFEFLIMDNQIREALANGASESQIRTMIRQKGYGSLLS
                     SGLARAIEGRTTAEEIIRVTYAENIEV"
BASE COUNT         1665 a         1512 c         1538 g         1587 t
ORIGIN      
        1 cgagtgcctt aacctcgcag cccagctcga aatacctgcg aattttggca tcatctaaga
       61 tgccgtagca gtcaataaca gccagcgggc cgccgaccca gttgacgatt tgatcgggtt
      121 ccagattcag atagggtgca tgcggaacag ccagtattac tgcttctgcc ccttttaatg
      181 ccgctggaag gtctttttcg acgcgtagat ttaccaaatc atcctgattg cggaaaaacc
      241 gtgcccatga atgtcccgga gccggatact catcctgctt ttccaattca taccagtgtt
      301 cgacatacgg gtcgtgaacc tttacatcag cccccatctc tgtaagcttt cgaacaacaa
      361 tttccgatcc gctgtaccgc gtatctgcaa cgtcctgacg gtagctggcc ccgcacagga
      421 ggacagatgc ccctgctata tagcgcccca tattccgcaa agcatcacgt gtgagggtgg
      481 ctgcatgaag tccgcgtgta tcgttgatat cgatggcggc ggtagtcagc ttgaaaatat
      541 catcattgaa tcccagaata tgcctgtatg cccaatatcc taatcctccg tctttgggca
      601 gacagtagcc gccgataccg gggcccggaa aaataatatt gctgtgtgtc ggccgcattt
      661 tgatcgcctt gataactttg attaaatcaa cgccgttgcg ctctgcaaat aagctccatt
      721 catccagaaa agccaagata actgcgcgat aggaattttc aacaattttg gttgtttcgg
      781 attcaatcgg acggtccatg actgtcagcg gatatttctc tgtattgaga acttcccgca
      841 gaaacttttc cacccggatt ttggcggctt cattgcatcc tgaacaaacc cgccagaaat
      901 cccgtattga cgatacgtac tctctgccgg gcatcacccg ctcaaaacta tgtgccagca
      961 gcggttcgga tgcaatgcct cgtgctttga atgccttctt cagaatcggc catgccacaa
     1021 attcggttgt ccccggcgcc acagtagtct caatcagcgt caggcaattg ggctgtactt
     1081 tttcaccgat tgtcttaagc gtagcttcca atgctttcat ctctgcctcc cctgtccgca
     1141 ggttgcccag cgaacgcttg ctgaaatcac actgtacatc tactactacg cagtctgcca
     1201 gcgacaggca gtcgctgttg tatgtggcga ccaatgtttt tttgtccttc acgcatcggg
     1261 cgatcatcgg gtccacttgc ggatcttcgg ctttcaccgg tgactgcccc ctgttgagca
     1321 tcgggatttt ccagtagctg cgggtactgg gccgctggca gccgatgaca aatttactcg
     1381 gcttgccggt ttttttatca actgtatcag caataattgc tgccattacg gccccgacaa
     1441 atccaacacc catgaccacg acgatttctt tgccttcttg acgtgcggtt gagactaaag
     1501 tttcgagacg cttaaattca gcagggtact cctcgggttt gggaagcggg aatttctcgc
     1561 cggacgggct gacagaatag tcgttcataa gcgatctccg tataagttgt tcgaaaccag
     1621 atacttaccg ttttaatcgg taagttgact ggacaagttt aaataaaata gttagttttg
     1681 gataataaaa agctgtaaaa tttttacaaa agaaaagtga tggttgatgt cattatttca
     1741 ttattgcggc aagtgatgta aattgaagga cctatggaat tgatagacaa tcaaaccaga
     1801 gtgtgtgtat taaccggccg gaaaatggcg gctattgcca gcattgctct ggaaggaccg
     1861 gagtcgcaaa ctatacttgg aaaaattttc agaaaaaatc cttttaataa gcatcgatcg
     1921 aatactgctg cagacaccgg cgttatagat cttcagcagg tcctatatgg ctgcattatg
     1981 gatggcagtc aagtgattga tgaagcagtt gttggatgtg aagaaaagga ttcttatgtt
     2041 attcattgcc acggaaatcc tctgctggtt gagcgaatta tcaatttgct tcaatcctat
     2101 ggagcacagc ttacagaagc ggaaaccttt tttttaaggc gctatcgacg gcagtccggc
     2161 tctttgattg aagccgaagc caaattggcg atgcagactt catataccat tgccggcgtg
     2221 cggatacttc aagcccaaat tgctgccggt ctgacattgt gggcaggccg tatgatggat
     2281 gccgtcaata tactgacacg cgagcaaatt caaaatgaat gccgtcagat tttggcaaac
     2341 agcatcattg cccgacggat cattcacgga gtacgtatcg tcatagccgg cccccccaat
     2401 tcaggcaaaa gcaccctttt gaattgcttg gcagggcggc aggaagctat tgtctcggac
     2461 atcgccggaa caacgcggga ttggatcagt attgtctgcc ggatggaacc gctgtatgca
     2521 gagtttattg atacagccgg attggatacc tcattggcac aagattatgc tcttcagcgg
     2581 catgctcagc agattacaca gcaattgctg gaaaactgtg atttaattct gcatctgcac
     2641 gatgctgctg cgttggaaga tagcggtatt caatcaatta atacaaatgt aattgcaaat
     2701 ttcgctcaaa aaaagcccat tattaatatt tataataaaa ttgacctttt aaaggataga
     2761 aatattcctc actgtctgcc aaacggcctt tgcatcagtg ctaaagaggg catagggatt
     2821 gaattgcttg gccggacgat aggaaaaaca ctaggcacaa acgattttga tgcaaatcag
     2881 ccggtcgtgt ttacagaccg ccagagagcg ataatatcac aaattatgaa ctccgcagaa
     2941 tcccttagag atttattatc ggacttgatt gggctttcgt atccctccac gcattagcat
     3001 acgaactgtc cggtatgcaa ttttagcgat tcgatgattc agtcagtctg tttgattcac
     3061 tcaatccctt caaaataagt ttttttaagt taattgactg gcggtttatg acgacagaca
     3121 gatgtacccg tatgtgtaca gattcatgca gggaaactgt cggatttaat cggtaacagc
     3181 ataaggacta aattgtggca caagaacaac aggacaaaaa agatacccca gcagccgatg
     3241 ccatgcaaaa acagattgcc ctgcaggaag cgcaatttta tgccaaaggc cggctgatga
     3301 acaatatggc ctaccaaatc cgcacattgt ctaacgccat tgtcggattc agcgatttac
     3361 ttctttctga accgctcagt ccagatcaat tggagtatgt gcgagaaatc aatcaggccg
     3421 gcaacggttt atcggcactg gtcaatgagg tactggattg gactcaggta atgaccggcc
     3481 ggattgcggt ttccaaaaca tcatgcgatc tggcagagct tcttgggcgt ctggaggcaa
     3541 tcttgcgttc agccgcacgg gaaaaagggc ttgcctgtgc tgtgcagctt gatccgcagc
     3601 tgccggattc tattgtttgt gattcggaac atctgtataa atgcctcctc aatttattgt
     3661 ccaatgctgt caagtatacg cgcaaaggat ccattcggat tcatgtgcga ttggatgaca
     3721 gtcaggccga accggcagtt tgttttgatg tgatagatac cggtatcggc atagaggcag
     3781 ataaaatcga ctctattttt gatccggctc agaagcggga agaatcaaat gaacaagtcc
     3841 aaacactttt taatctcggc ttgacagtaa cggcagggct gtctttaacc aagcagttat
     3901 gtcatctgat aggcggaaca atagaggtaa ccagccaagt gaatagcggt tcaacgttta
     3961 cgctgcgaat ccccgcagga ttggaattgt ccacccggca ggagtccccg atatacagcc
     4021 atccaaagca ggagacacag aagtctgcct ctgagccggc tttgatactg ctggcagaag
     4081 accagccgtc caaccgcacg gttattacct taatgctcga agcaatgggc gttcgcgtag
     4141 acacagctca ggatgggcgg gaggctgtcc aaaaagccct tcagaatact tatgacctga
     4201 tactgatgga tttaaaaatg ccgacgatgg acggctatga agcagcggca caaattcgcc
     4261 gccagaatca cgtccccatg gttgccttat cggccaaggt gtttgatgaa gatgaacatc
     4321 gccgcatcac agctttgttt gaaggatttc tcaccaagcc tgttgacggc cgcaagcttg
     4381 cacagacact cgaacaattt atagattcgt ttcagtattc cgcaccgacg gccgcagcct
     4441 cggcaggccg tccggcagaa aacatagcat atgaatacag aacataaacc agaccagaaa
     4501 tgacagcaaa cgtatccaga caagtgcagt attccggtat gccgctggcc gtcaaggaga
     4561 atcagatgtt tggtgactgg ctggttcaga acggctatat cacggaacag cagcttgccg
     4621 cagcactgaa cgaccagaaa accagcggag gccggctggg acagaatttg gtgcgtctgg
     4681 cctatctaaa cgatgaacag ctgacgcagt gtctggcggc ttatctgaat ctggagtcca
     4741 tatcgctgtc agatctttct gttattaaca aggaagtagc acgcagaatc cctgaaaata
     4801 ttgccaaacg cttccatagc ttggctgtcg atgctaaaga cgaccgcatt cttgtggcaa
     4861 tggccgatcc gctcaatgta attgccgttg atacggtttc agtcaagctg aagaaaaata
     4921 ttcaggttgt gctggcttcg gaacgggaaa tccagcgggc cattgagtat gtctaccatg
     4981 gaacggatgt cgaagaacag cagcttcgcg acttggtcga attggaaatc agcagcgatg
     5041 aagacgacct gatggaaaat acgctggcta tggaagtgtc cgctgcggcc gaggcacagg
     5101 atgcaccggt gatccgcttt gtggatcttc tgcttagtca ggccattaaa agccgagcca
     5161 gcgatattca tatcgagccg caggataaat cgatgagcat ccgcatgcgg attgacggac
     5221 tactgacgga aatggtgccg ccgccaagaa aaatgcagcc ggccgtaatt gcccgcatca
     5281 agatactttc cgaaatggat attgccgagc ggcggctgcc gcaggatggc cgttttaaaa
     5341 tcagacgggg ccagggaaaa gaagaaattg atgtccgtgt ttcttcgctg ccgactatct
     5401 atggcgaaaa aattgtaatg cgcatccttg ataagggagc agtcacccat aatcttgaca
     5461 gtctggggct ggagcctgac ctactggcta aatttaagat gacgctccag cagccccatg
     5521 gcatcatcat cgttacaggc cccaccggca gcggcaagag tacaaccctt tattcagcgc
     5581 tcaatgagct gcgggacccc cacaaaaaca ttacgacagt cgaggatcct gtggaatacc
     5641 ggcttgaggg aattaaccag acacagatta agccgtctat tggtctgacg tttgcctcat
     5701 gccttcgaac gattctgcgg caggatcctg atattatttt gattggcgaa atccgcgata
     5761 aagaaacgat ggaaattgct atcaaggcct cgctgacggg acatctggta ttgagcacct
     5821 tccataccaa tgatgccccc agtgccttga gccgattggt ttatatgggt ctggagccgt
     5881 atctgctggc ctcttctctg aacctgatat tggcgcagcg gctcgtgcgc cgcatctgtg
     5941 acaaatgcaa agccccccag acactgacag aaactcagat taaacgtctg gaattaacgc
     6001 ccgaacagat tgccaacaca acattctatc atggcaaagg gtgttcggag tgcggcggaa
     6061 gcggctaccg cggacgtctt cccatttttg agtttttgat catggataac cagattcgcg
     6121 aagctcttgc caatggggct tctgaatcac agattcgaac gatgattcgc caaaaaggat
     6181 acggcagtct gctcagcagc ggtcttgccc gtgcgattga aggccgcaca acggccgaag
     6241 aaattatccg tgtaacttat gcagagaaca tagaggttta gctgtttttt tttttttttg
     6301 tt
//
LOCUS       DAOTBO010000133         6255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10576_length_6255_cov_4.591613, whole genome shotgun sequence.
ACCESSION   DAOTBO010000133 DAOTBO010000000
VERSION     DAOTBO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10576_length_6255_cov_4.591613"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..640)
                     /locus_tag="PLE88_11585"
     CDS             complement(119..640)
                     /locus_tag="PLE88_11585"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOM77147.1"
                     /translation="MAAETISNEELVKLAEALDGATQAKVIAASDVQTAAWVRLKCQY
                     GCDGYGQCLVCPPYSPSPEQTQQVLDGYRRAVLVHFKDWNNTKPQMVKLERTVFLKGA
                     WKAFAMGAGPCRFCAVCTLQIGQCRHAEQARPAMEACGIDVFTTARKAGFPIEVVKNR
                     RQCPNFYGLLLVD"
     gene            complement(809..1453)
                     /locus_tag="PLE88_11590"
     CDS             complement(809..1453)
                     /locus_tag="PLE88_11590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOM77148.1"
                     /translation="MKKGVIFDLDGTLVNTLADLTNAMNFALNELGCPARSSEECRQM
                     IGRGLRNFAYEALPDDKKDLCGQLVERMVSRYQQHCLEETTVYDGIYETVEELARRGL
                     RLAVLTNKKQKPTDLIIRHFFKPGLFNPVIGENDGRKVKPDPQTTLEIIAGWGLTSHE
                     ILYAGDSDIDIHTARAAGVQCVGCEWGFRSRRQLLDAGAEILIQQPQQLLNLLD"
     gene            1606..2121
                     /locus_tag="PLE88_11595"
     CDS             1606..2121
                     /locus_tag="PLE88_11595"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOM77149.1"
                     /translation="MQVNLVFLKKDGTIQSFPLPSDVTFIGRRQDCDLCIPLPMVSRR
                     HCQIYTEQGNLMVRDLHSRNGILLNDQPVEEARIKAGDVLKIGPIRFVFQIDGKPDSF
                     EQYFQPPHAPSIQPQAAPVRLERPQPSDLEKPQGLAALAGQSQTMDMDGVLSEDIFTE
                     EDFDLDSDLPA"
     gene            2169..3224
                     /gene="rlmN"
                     /locus_tag="PLE88_11600"
     CDS             2169..3224
                     /gene="rlmN"
                     /locus_tag="PLE88_11600"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOM77150.1"
                     /translation="MDRDLKNRTHHELSELAAAYGQKPFAADYLFSFIHQKDAEQISA
                     VSPLSKQFRQKLADEGFFIGSIGLLEQQEDPDGTVKFVFGMADGVRIESVRLKDGRRN
                     TLCISTQAGCRMGCRFCATGQLPYRRNLTAGEIADQVYKAEALVGRIDNVVYMGMGEP
                     LDNYDNVMRSVEILNDKCGRNIGIRHITISTCGLPEAIQKLAKEKLRPRLAVSLHAAS
                     DAVRSKLMRIGQKYPLSALLASLKTYQSITGSRITVEYCMIAGVNDQPEQAAMLIKLL
                     RPLKVNVNLIELNAFPGCRFEASPSGKIQAFADMLADAGIETVIRFKRGRKIKAACGQ
                     LGADWLQKQSGPTLQGT"
     gene            complement(3268..3855)
                     /locus_tag="PLE88_11605"
     CDS             complement(3268..3855)
                     /locus_tag="PLE88_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77151.1"
                     /translation="MINRPYITIVSGLPRSGTSMMMRVIHAGGIEPLTDHIRKPDEDN
                     PKGYYEFEPVKKTKQDASWLKDAPGKAVKMVYRLLYDLPDAYEYRVVFMQRDLTEVLA
                     SQKKMLERSGKTSGGVSDQTMAVLFAAELDKCQKWLASQPHFRVLYISHRQMIDNPLP
                     QCQTINAFLDGGLDVNAMAAAVDPSLYRNRNSPGR"
     gene            4009..5199
                     /locus_tag="PLE88_11610"
     CDS             4009..5199
                     /locus_tag="PLE88_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOM77152.1"
                     /translation="MKTYEQINDRIASGKAVVLTADEIMDYVDRKGLEKAAQEVDVVT
                     TATFGPMCSSGCFLNFGHSRPRIRMTEAWIEDVLVYTGIAAVDVYLGATQLRHGDPAN
                     MYYPGEFRYGGGHVIEDLVAGKTLQLFALSYGTDCYPLREIRTYFTIHDLNQAIMVNP
                     RNCYQNYNVALNKSDRPIYTYMGLLEPDCKNANYCSAGQLSPLLNDPYYETIGVGTRV
                     WLAGAQGHVYAEGTQHAGGCERTADGIPIGGAGTLALTADMKQMNKAFVRGVSLPGYG
                     VSLALGVGIPIPILNEQILQRTCVRDRDIWAPVVDYSSDYSQNTGKIVCQVNYEQLRS
                     GRIQIDGKTVEVGSLSSYYKALEIAHLLADEIRRGDFLLAQPIAPLPKDTKMKPLVKR
                     KPPL"
     gene            5196..5615
                     /locus_tag="PLE88_11615"
     CDS             5196..5615
                     /locus_tag="PLE88_11615"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOM77153.1"
                     /translation="MSETMTRKLMLYFPKCECEKPIIYHLVKDYGLIVNVFRAKVTPE
                     EEGYLVLDVTGTEEQIQRGIEFVKTFNVTVNFSGKGVTWDSIRCTHCGYCVPYCPTGA
                     LKIADAATREVAYVESECIECLACVRLCPYGACASAF"
     gene            5590..>6255
                     /locus_tag="PLE88_11620"
     CDS             5590..>6255
                     /locus_tag="PLE88_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOM77154.1"
                     /translation="MGRVLRHFNRVDSKRIYNTFVHKEAVFRICCSCYEAVTLEIIRQ
                     RKILEEYIHRHPEFRDSLEPVQLLAHPPESACRMAQAARRTGTGPMAAVAGAMAQLAA
                     EAGLKAGAEEAIVDNGGDIYLRTVKPVIIGLYAGGSAAVNRLAFEIQPEDTPLAICSS
                     SGLMGHSMSLGRCDLATIAARDAALADAAATLAANLVRTEADVEPALQRVMSIEGIDG
                     VLIV"
BASE COUNT         1383 a         1645 c         1804 g         1423 t
ORIGIN      
        1 agcccgtccg gccgatgata gtccttcggg gcgaaagtag gtgattgcca ggatttataa
       61 ggccgctgcg ggaaactgca gcggcctttc cttttgcgcc ggatgccgcc gcagccggtt
      121 aatcgaccag cagcaggccg tagaagttgg gacattggcg gcggtttttg acgacctcaa
      181 tgggaaagcc cgcttttctg gctgtcgtga agacatcaat gccgcaggcc tccatcgccg
      241 ggcgggcctg ctcggcatgg cggcactggc cgatttgcag cgtgcagaca gcgcagaagc
      301 ggcacggacc ggcgcccatg gcgaatgcct tccaggcgcc tttcagaaag acggttcgtt
      361 ccagcttgac catctgcggc ttggtattgt tccagtcctt aaaatgcacc agcactgccc
      421 gccggtagcc gtccagcacc tgctgcgtct gttcgggcga tgggctgtag ggcggacaga
      481 ccagacactg gccgtagcca tcacagccat attggcattt gagccgaacc cacgcggccg
      541 tttggacatc ggaagcggca atcacttttg cttgtgtagc gccgtccagc gcttcagcca
      601 gcttgaccaa ttcttcattg gaaattgttt ctgcggccat acccgactgt tcctttccaa
      661 aagtgaacct ttgaaatcat tccgttttca cggctggtgc tgaacagtat aatgctgtca
      721 attcttgtaa tgaaataaaa aaacgtcttc gaaagcagca gaccattcat tgcaatccca
      781 ttaaaaaggc acaggcagca gatgccggtc aatcgagcag attcaacagc tgctggggct
      841 gctggatgag gatttcggcg ccggcgtcaa gcagttgcct gcgggagcga aagccccatt
      901 cacagcccac gcattgcaca cctgcagcac gggcggtatg aatgtcaata tcgctgtcgc
      961 cggcgtacaa aatctcgtgg ctggtcaatc cccatccggc gatgatttcc agagttgttt
     1021 gcggatccgg cttgaccttg cggccgtcgt tttctccgat gacgggatta aacaggccgg
     1081 gtttgaagaa atgccgtatg ataaggtctg tcggtttctg ctttttgttg gtaagcacag
     1141 ccaaccggag gccccgtcgg gcaagttctt cgactgtctc ataaatgccg tcatacaccg
     1201 ttgtttcttc caaacagtgc tgctgatagc ggctgaccat gcgttcaacc agctgcccgc
     1261 acaaatcctt tttgtcgtcg ggcaatgcct cgtaagcgaa attccgcagc ccgcggccaa
     1321 tcatctggcg gcattcctct gacgaacggg caggacagcc gagttcattc agggcaaagt
     1381 tcatcgcatt tgtcaggtcg gccagcgtgt tgaccaatgt gccatccaaa tcaaaaataa
     1441 cccctttttt caagaaaacc atcctttcgt gtcgtcaata aagtatccgg ataattattt
     1501 gagacaatca atagtttccg cttgtaatac tggaactata tactataatt gatgttatat
     1561 tccaaacgag attttggaat tgccggacaa actcgggagg tgccaatgca ggtcaattta
     1621 gtgttcttaa agaaggatgg aacaattcaa agcttcccgc tgcccagtga tgtaacattt
     1681 atcggccggc ggcaggattg tgacctttgt attcctctac ctatggtgtc ccgccggcat
     1741 tgccagatat acaccgagca gggcaacctg atggttcgcg accttcacag ccgcaacggt
     1801 attctgctca acgaccagcc ggtcgaagaa gcccgcatca aagccggaga tgtattgaaa
     1861 atcggtccga ttcgctttgt tttccagatt gacggcaaac cggacagttt tgagcagtat
     1921 ttccagcccc cccatgcccc atccatccag ccccaagcgg ctccggtccg gctggagcgt
     1981 ccgcagccgt ccgatttaga aaagccgcag ggattggcgg cccttgccgg ccagagccaa
     2041 acaatggata tggatggcgt gctgtccgaa gacatcttca ccgaagagga ttttgatttg
     2101 gattcggacc taccggcgta gcgataaaac aattgcctgc agctgtctgg tgcggataca
     2161 atatccgtat ggacagagac ctgaaaaatc ggacacatca tgagctgtca gagctggccg
     2221 cagcctatgg gcaaaagccg tttgcggcgg actatctttt ttcatttatt catcaaaagg
     2281 atgccgagca gatatcggct gtcagtccgc tgtcaaaaca attccgtcag aagctggctg
     2341 atgagggatt ttttatcggc agcattgggc tgctggaaca gcaggaagac cccgacggta
     2401 cggtcaagtt tgtctttggc atggccgacg gcgtgcggat tgaatccgtg cggcttaaag
     2461 acggccggcg caataccctg tgcatttcga cgcaggccgg ctgccggatg ggctgccgat
     2521 tctgtgcgac gggtcagctg ccgtatcggc ggaatctgac cgccggagaa attgcagatc
     2581 aggtttacaa agccgaggcc cttgtggggc gaatcgacaa tgtggtctat atggggatgg
     2641 gcgaaccgct ggacaactac gacaatgtga tgcgttcggt ggaaatcctc aatgacaaat
     2701 gcggacggaa catcggcatt cgccatatca ccatctccac ctgcggcctg ccggaagcaa
     2761 ttcaaaagct ggcgaaggaa aagctccggc cgcgattggc ggtgtcgctg catgcggcca
     2821 gcgatgccgt tcggtcaaag ctgatgcgga ttgggcaaaa gtacccgctc tcagcgcttc
     2881 tggcatcact gaaaacctac caaagcatca ccggcagccg cattacggtc gaatattgca
     2941 tgattgccgg cgtcaatgac cagccagaac aggccgcaat gctgatcaag cttctgcggc
     3001 cgctgaaagt caatgtgaat ctgattgaat tgaatgcatt cccgggctgc cggtttgaag
     3061 ccagtccgtc ggggaagatt caggcgtttg cggatatgct ggccgatgcc ggaatcgaga
     3121 cggtcatccg gttcaagcgg gggcggaaga ttaaggccgc ctgcgggcag ctgggcgccg
     3181 actggctgca aaagcagtct gggcccacac tgcaggggac ataagccggc agtgccggct
     3241 gccggtgtct ttttgccgga tggctgatta gcggccgggg ctgtttcggt tgcggtacag
     3301 cgacggatcg acagcggcgg ccatagcgtt gacatccaat ccgccgtcga gaaaggcatt
     3361 gatggtctgg cattggggca ggggattgtc gatcatctgg cggtggctga tatataaaac
     3421 cctgaaatgg ggctggctgg ccagccattt ttggcattta tccaattctg cggcaaacag
     3481 aacggccatc gtctggtcgg aaacgccgcc gctggttttg ccactgcgtt cgagcatttt
     3541 cttctgcgag gccagcactt cggttaaatc ccgctgcata aataccaccc ggtattcata
     3601 cgcatcgggc aggtcataca gcagccggta caccatcttg accgccttgc cgggggcatc
     3661 tttgagccac gaggcatcct gcttggtttt cttgaccggt tcaaattcgt aatagccctt
     3721 gggattgtct tcatccggtt ttcggatatg gtctgtcagc ggctcaatgc cgccggcatg
     3781 gatgacccgc atcatcatcg atgtcccgga gcggggcagg ccggagacga ttgtaatata
     3841 cggtcggttt atcataagtc cctttgcatc tgtgtttttt atatagtaaa ccaaatgcat
     3901 cggcatttca aacagttttt gctgctcttt cagaagcaat aaatcttctt gataaacagc
     3961 ggcggtgtgg atacgatgaa gacggtgctt ttgaggataa aaagacttat gaagacatat
     4021 gagcaaatca acgatcgcat tgcatccggc aaagccgttg tcctgacggc ggatgagata
     4081 atggattatg tggaccgaaa gggtctggag aaagcggccc aagaagtgga tgtagttaca
     4141 acggccacct ttggcccgat gtgttcgtcg ggctgctttt tgaacttcgg ccattccagg
     4201 ccgcggatac ggatgaccga ggcctggatt gaggatgtac tggtctatac cggcattgcg
     4261 gcggtggatg tctatctggg ggctacccag ctgcgtcacg gggacccggc taatatgtat
     4321 tatcccggcg agttccgcta tgggggcggg catgtgattg aagaccttgt ggcgggcaaa
     4381 acgctgcaac tgtttgccct gtcgtacggc acggactgct atccgctgcg ggaaatccga
     4441 acctacttta ccattcacga cctcaatcag gccattatgg tcaacccccg caactgctat
     4501 cagaattaca atgtggcgct taataaatca gaccgcccga tttataccta tatggggcta
     4561 ctggagcccg attgcaagaa tgccaattac tgctcggccg gacagctttc gccgctgctg
     4621 aatgacccgt attatgaaac aatcggcgtg ggcacgcgtg tttggctggc cggtgcgcag
     4681 gggcatgtgt atgccgaggg cacacagcat gccggcggat gcgagcgtac ggccgacggc
     4741 attcccatcg gcggcgccgg aacgctggcg ctgacagcgg atatgaagca gatgaataag
     4801 gcgtttgtcc gcggcgtcag tctgcccggc tacggggtgt cgctggcgct gggcgtgggc
     4861 attcccattc ctatcctcaa tgagcagatt ctccagcgca cctgcgtgcg cgaccgcgat
     4921 atttgggcgc cggttgtgga ttacagttcg gattattcgc agaataccgg caagattgtc
     4981 tgtcaggtca attatgagca gctgcgcagc ggccgcattc aaattgacgg aaaaaccgta
     5041 gaagtgggct cgctgtcgtc gtattacaag gcgctggaaa tagcccatct gctggccgat
     5101 gaaatccgcc ggggcgattt tctgcttgcc cagccgattg ccccgctacc caaagacacg
     5161 aagatgaagc cgctggtgaa aaggaagccg ccgttatgag cgaaacgatg acccgaaaac
     5221 tgatgctgta ttttcctaaa tgcgaatgtg aaaagccgat tatttatcat ttggtcaagg
     5281 actacggcct gattgtcaac gtctttcgcg ccaaggtgac gcccgaagaa gagggctatc
     5341 tggtgctgga tgtgaccggc accgaggagc agattcagcg gggcattgag tttgtcaaaa
     5401 ccttcaatgt cacggtgaat ttcagcggca aaggcgttac atgggacagc atccggtgca
     5461 cccattgcgg ctactgcgtg ccgtattgcc cgaccggagc gctgaagata gccgatgcgg
     5521 caacccgcga ggttgcctat gtggaatccg aatgcattga gtgtctggcc tgcgtgcggc
     5581 tgtgtcccta tggggcgtgt gcttcggcat tttaatcgcg tggactccaa gcgaatctac
     5641 aacacatttg tccataaaga agcggtcttt cgcatctgct gcagctgcta tgaggcagtt
     5701 acactggaaa ttatccgcca gcggaaaatc cttgaagaat atatccatcg gcatccggaa
     5761 tttcgcgatt cgctggagcc ggtccagctg ctggcccatc cgccggaatc ggcctgccgg
     5821 atggcacagg ccgcccggcg gacaggcacc ggaccgatgg cggcggtggc cggagcaatg
     5881 gcccagcttg cggcggaagc aggcctgaaa gccggcgccg aggaggcgat tgtggataac
     5941 ggcggcgata tttatcttcg caccgttaaa ccggttatca tcggtcttta tgccggcggt
     6001 tcagcggcgg ttaaccggct ggcctttgaa attcagcccg aagatacccc gctggcaatt
     6061 tgttcttcgt ccggcttgat gggacattca atgagtctgg gccgctgcga tttggcgacg
     6121 attgctgcca gagatgcggc gctggccgat gccgcggcga ccttagctgc caaccttgtc
     6181 cgcacagagg ccgatgtgga accggcactg cagcgggtga tgtccatcga gggcattgac
     6241 ggggtgctga ttgtc
//
LOCUS       DAOTBO010000134         6158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10765_length_6158_cov_5.031624, whole genome shotgun sequence.
ACCESSION   DAOTBO010000134 DAOTBO010000000
VERSION     DAOTBO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10765_length_6158_cov_5.031624"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..298
                     /locus_tag="PLE88_11625"
     CDS             110..298
                     /locus_tag="PLE88_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77155.1"
                     /translation="MIGQECASKPDVNHQVDELNSQAQKHLDDLQETQKQIHIVSLKA
                     DVARRGIKSKISELSEQE"
     gene            302..442
                     /locus_tag="PLE88_11630"
     CDS             302..442
                     /locus_tag="PLE88_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77156.1"
                     /translation="MRFEKLFPTLLIILDVCAALAYLPSGDWRHVVYWLAAAILTTCV
                     TY"
     gene            445..2163
                     /locus_tag="PLE88_11635"
     CDS             445..2163
                     /locus_tag="PLE88_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOM77157.1"
                     /translation="MQLRDYQQQSVHAVYDYLRNRDGNPCIVIPTAGGKTPVMSTICT
                     DVVNLWGGRVLVLANVKELLEQTAGTLSQIAPDLDIGIYSAGLNRRDTEHSILVAGIQ
                     SVYKRACDLGAFDLVLIDEAHMIPPSGEGMYRTFLQEAVVVNPNLRVIGLTATPFRMT
                     SGMICGPDNLLNEICFEVGVRELIVQGYLCPLKSKAGRQKADTSGLHLRGGEFIASEV
                     EDLMDQDALVSSACSEIIEHTASRNSVLVFAAGISHARHIQTLLQQRTRQEVGLVTGD
                     TPAGERAELLARFKRQIVKADLFGNNKPPLKYLVNVNVLTTGFDAPNIDCVVLLRPTN
                     SPGLYYQMVGRGFRLHPSKEDCLVLDFGGNILRHGPVDSLQIKDRSSGNGDAPAKECP
                     ECHSVIHAAYATCPECGYEFPPPERQQHDARASTAGILSGQTEDTDYQVYDTLYHYHM
                     KRNAGPDDPPTLRVEYNIGFNQWISEWVCLEHTGYARNKAEGWWRARSNEPVPNTVQQ
                     AIELAEVGALAPTEQITVRSITGQKYDRIINYRLGTKPPLLDGSQERDDGNLPDYSWA
                     EDDIPF"
     gene            2176..2613
                     /locus_tag="PLE88_11640"
     CDS             2176..2613
                     /locus_tag="PLE88_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77158.1"
                     /translation="MTTFYKGAFERCSWCNGRGCNQCHLERKKYEDQFKTPQPLFSAD
                     VNDPQDMQLLKEVFGRQALEHAFGPDGDGMQEIEQAAALASFKQAMRKHNKTAPTMAC
                     PQCGAEYEDFDGLGVLHCEHCGYCKHASITDCQCDYCGQAQGT"
     gene            2617..4851
                     /locus_tag="PLE88_11645"
     CDS             2617..4851
                     /locus_tag="PLE88_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOM77159.1"
                     /translation="MNHHIQQASLHYLQQGLSVLPAKRVDKRPCIGSWKQYQQRLPTQ
                     MELDAWFSNPQDGICIITGRISGNLEIIDFDNGGELFDAWYALVPADLRNRLVVEQTP
                     SGGWHVIYRCESAISGNMKLAQRKDGSKIHTLIETRGEGGLFLCAPTAGYELMQGELT
                     ALSVLAESEREILLEAAWSLNEHIPEPIQHSQHSDGSALRPGDDYNARGNVIDLLKSH
                     GWQFVNASGDNQRWRRPDKAKGWSATLRTTDNVFYVFSSNAGPFESNKAYSPFMMYTI
                     LEHGGDFSKAALALSKQGYGQPVSSPETDDVDLSGILSPPEDKPKTELFDPGQLPDEL
                     LRIPGFVSELMDFCMQTAPYPNLGLAFCGALSMQSFLCGRKIRDPGNLRTNIYLLALA
                     GSSSGKDWPRQINAHIFMEIGKTDALGDKFASGEGIQDAMNLTPSMLFQNDEIDGILI
                     SLNKSRDGRFESIMGTLLTMYTSSGSVYPMRRKAGKQSGGIIDQPHLTIFGTATPKYY
                     YESLSERMLSNGFFARMMVVDIGYRGTGQDAGLVDTMPKRILETAKWWDDFCPGQRRG
                     NLISFHPKPEVITMTDAARDILDEFRYTADSEYAKSEAENDESAKSIWGRANENARKL
                     ALLYAASERYQSPEISQQGATWAVRFAEHQIRRMLYLVGQHVFENEFDKKCKRFLQVI
                     ANWQKKKGTEWMPHWYLARLLKGWSEKDIEMVREALVARLEIAVEVGSTRKGGPVGYR
                     YKPL"
     gene            5198..>6158
                     /locus_tag="PLE88_11650"
     CDS             5198..>6158
                     /locus_tag="PLE88_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOM77160.1"
                     /translation="MSKNFTIELRNIDAIHPYDKNPRINDGAVDAVAASLKEFGFRQP
                     IVVDKDGVIVCGHTRYKAALQLGLKKIPVHVATDLTPEQIRAYRIADNKTADLAEWDY
                     NILPIELSELQDAGFDMDLLAFDEKELVKLLDTDVTQGNTDLDAVPEPPDEAITQLGD
                     VWVLGNHRLMCGDSSSEADLDTLLDGKTIDLVNMDPPYNVKVEPRSNNAIAAGLSSFG
                     KGLQHHQSFDVARDKTKAQKTTPKLRAKDRPLENDFVSKEDFDEMLLAWFNNASRVLR
                     PGGSFYIWGGYANLGNYPGPLKAAGLYFSQGIVWDKQHPVLTRK"
BASE COUNT         1565 a         1522 c         1623 g         1448 t
ORIGIN      
        1 aaaaaacgtt aactgagatt ttgatcctca aaaatcaaat tattagaggt tcccggaaat
       61 tgtaattgct ctcattttgg gtgcttgtgt aggactgctc atagggatct tgatcggcca
      121 ggagtgtgcc tccaagcccg atgtcaacca tcaagttgat gagctgaaca gccaagccca
      181 aaaacatctg gatgaccttc aggaaacgca gaagcagatt cacatcgtca gcctgaaagc
      241 tgatgtggct cggcgcggga ttaaatccaa gatcagtgaa ttgagcgagc aggaataggc
      301 aatgcgattt gaaaaactgt tcccaacctt gttaatcatc ctggatgtgt gtgcggcgct
      361 ggcctattta ccttcgggcg attggcggca tgtcgtgtac tggctggccg cggcaatcct
      421 gacaacctgt gtaacctatt gagcatgcaa ttacgagact atcaacaaca atcagtccat
      481 gcggtctatg attatctccg gaatcgggat ggcaatcctt gtattgtcat cccgaccgct
      541 ggaggtaaga ccccggtcat gtcgactatc tgcacagatg tggtcaacct atgggggggc
      601 cgcgtactgg tattggcgaa tgtcaaagaa ctgctggaac aaaccgcagg cacactcagt
      661 cagattgcac cggatttgga tattgggatt tattcagcgg gcttaaatcg tcgtgatact
      721 gaacacagca tcctcgtcgc aggtattcaa tctgtctata aacgagcgtg tgatttgggt
      781 gcattcgatt tggtcctgat tgatgaggcc cacatgatcc ctcccagtgg cgagggcatg
      841 tatcgcacat tcctgcagga agcggtggtg gtcaatccga atctgcgagt catcggtcta
      901 acggccacac catttcggat gacatcaggt atgatctgcg gtccggataa tctgctcaat
      961 gaaatctgct ttgaggtcgg tgttcgggaa ttgattgttc agggttacct ttgtcctttg
     1021 aaaagcaagg ccgggcggca aaaagctgac acctcaggct tacaccttcg gggtggtgaa
     1081 tttatcgctt cggaagtcga agatttgatg gaccaggacg cactggtttc ttcggcatgc
     1141 agtgaaatca tcgagcatac agccagccgg aacagtgtat tagtttttgc ggccggcatc
     1201 agtcatgccc gccatattca gacactcctg cagcagcgca cccgtcaaga agtcggatta
     1261 gtgacgggtg acacaccggc aggcgaacgt gcagaactgc tggctcgctt caaacgccaa
     1321 atcgtcaagg ctgatttatt cggcaataac aaaccaccgc taaaatacct ggttaatgtc
     1381 aatgtcctga ccaccggatt tgatgcccca aatattgatt gtgtggtatt gttgcgcccg
     1441 actaattcac cgggactcta ctatcaaatg gtgggtcgtg gatttcggct acatccatcg
     1501 aaagaggatt gtctggtcct ggactttggc ggcaatatcc ttcggcatgg gcctgtggat
     1561 agtttgcaaa ttaaggaccg ctcctccggc aacggggatg cacccgctaa agaatgtccc
     1621 gagtgccatt cggtcattca tgccgcctat gcgacctgtc cggagtgtgg ttatgaattc
     1681 ccgccgccgg aacgtcagca acatgacgcg cgtgccagta ccgccgggat tttgtcgggg
     1741 cagacggaag acacggacta ccaggtctat gatacgctgt atcactatca catgaagcgt
     1801 aatgccggtc ccgacgatcc gccaaccctg cgtgttgaat ataacatcgg atttaaccaa
     1861 tggatttccg aatgggtttg tttggaacat accggctatg cccgcaacaa ggccgagggc
     1921 tggtggcgtg cacggagtaa tgaaccggtc cccaatactg tccagcaggc tattgaactg
     1981 gctgaggtcg gcgcattagc accaaccgaa caaattaccg ttcgcagtat tacgggacag
     2041 aaatatgacc gcattatcaa ttatcgactc ggaacaaaac cgccgctgtt ggatggcagc
     2101 caagaacggg atgatggaaa cctgccggac tattcgtggg ccgaagatga tatccccttt
     2161 taggaaaaaa cttctatgac aacattttac aaaggcgcat ttgagcgttg ttcatggtgt
     2221 aacggacgtg gctgtaacca gtgccacctg gaacgcaaaa aatatgagga ccaattcaag
     2281 acaccgcagc cgctgttttc ggcagatgtc aatgatccgc aggatatgca gttattaaaa
     2341 gaggtctttg ggcgtcaggc cctggaacat gccttcggac cggatggtga tgggatgcag
     2401 gaaattgaac aggcggccgc tcttgcctca tttaaacagg ccatgcgaaa gcataataaa
     2461 accgccccca caatggcctg cccccagtgc ggcgctgaat atgaggactt tgacggtctg
     2521 ggcgttctcc actgtgaaca ttgcggttat tgcaaacatg cctctatcac ggattgccag
     2581 tgtgattatt gcggacaagc acaggggaca tagctggtga atcatcatat acaacaagca
     2641 tctttacact atttacagca gggattgtcg gtcctgcctg ctaaacgtgt ggacaaacgg
     2701 ccctgcatcg gctcctggaa gcagtatcag cagcgtctgc cgactcagat ggaactggat
     2761 gcatggttca gcaaccccca ggatggaatc tgcattatca caggccggat ttcagggaat
     2821 cttgaaatca tcgattttga taacggtggc gagttatttg atgcatggta tgcgcttgtc
     2881 cctgctgacc tgcggaatcg gctggttgtc gaacagacgc catctggcgg ttggcatgtg
     2941 atttatcgat gtgaatccgc catcagcggt aatatgaagc tggcccagcg caaagacggc
     3001 agtaaaatcc acacgctcat tgaaacccgc ggtgaaggcg ggctgttttt atgtgcaccg
     3061 acggccggat atgaactcat gcagggagaa ttgaccgctc tttctgtcct ggccgaatct
     3121 gaacgtgaaa tcctgctcga agctgcttgg tcactgaatg aacatatccc tgaaccgatt
     3181 cagcattccc aacattcgga cggttcggcc ttgcggccag gcgatgatta caatgctcgg
     3241 ggaaatgtca tcgacctgct caaatcgcat ggttggcagt ttgtcaatgc atccggtgat
     3301 aatcaacgct ggcgcagacc cgataaggcc aagggctggt cagccacgtt acgcaccacc
     3361 gacaacgtat tctatgtctt cagttccaac gctggcccat ttgagtcgaa taaagcctat
     3421 tcgccgttca tgatgtacac cattctggaa catggtggtg attttagcaa agcggctctg
     3481 gcactttcaa aacaaggata tggtcagccc gtgagttcac cggaaacaga tgatgttgac
     3541 ctctccggta ttctcagtcc tccagaagat aaacccaaaa cagaattatt cgaccccggt
     3601 caattaccgg atgaattatt acgcattccg ggttttgttt ctgaactgat ggatttttgc
     3661 atgcaaaccg ctccctatcc gaatctggga ttggcattct gcggagcatt gagtatgcag
     3721 tcctttctgt gcggccgaaa aatccgtgac ccgggtaatc tgagaaccaa tatttatcta
     3781 ttagcgctgg ccggttcatc cagcggaaaa gattggcccc ggcagataaa cgcgcacatc
     3841 ttcatggaaa tcggcaagac cgatgcgttg ggtgataaat tcgcttccgg tgaaggtatc
     3901 caggacgcga tgaacctgac gccatcgatg ctgtttcaaa atgatgagat tgacgggata
     3961 ttgatttcgc tgaataaatc tcgcgatggc cgttttgaaa gtatcatggg cacactgctg
     4021 accatgtaca cgtccagcgg ctcggtctat cccatgcgtc gtaaagcagg aaaacaatcc
     4081 ggcgggatca tcgaccagcc acatttgacc attttcggga ccgctacgcc caaatactat
     4141 tatgaatcgc tcagcgagcg gatgctgtcc aacggattct ttgcccgtat gatggtcgtc
     4201 gacatcggat accgcggcac cggtcaagat gccggtctgg ttgatactat gccaaagcgg
     4261 attctggaga ctgccaagtg gtgggacgat ttctgccccg ggcagagacg cggtaatctc
     4321 atcagctttc accccaagcc ggaagtgata acaatgacag atgcggcgcg agatatactc
     4381 gatgaatttc gatataccgc cgatagtgaa tacgccaaat ccgaagcaga aaatgatgag
     4441 tctgccaagt ccatctgggg cagggccaac gagaacgccc gtaaattggc gctcttgtac
     4501 gccgcaagcg aacgatacca atcgccggag atctcgcaac agggcgcaac ctgggcggtc
     4561 aggttcgccg aacaccagat acgtcggatg ctctacctgg tgggtcagca cgtattcgaa
     4621 aacgaatttg ataagaaatg taagcggttt ctgcaggtga tagcgaattg gcagaaaaag
     4681 aaaggcaccg aatggatgcc ccattggtat ctggcccgcc tgctcaaagg ctggagcgag
     4741 aaggatatcg agatggtgcg agaagcgctg gtagcccgcc tcgaaattgc agtcgaagta
     4801 ggcagcacca gaaaaggtgg gccggttgga taccggtaca aacctcttta gttaccgcat
     4861 tttcaggtat gcggtaactg gcaaaaatgc ggtaactggg gggtgtttca gggtagttac
     4921 cgcataaaac agccggttac cgcatagtta ccgcataggc aaaatgcggc aactgaaaat
     4981 cttaagtctt tatataataa taagttaata tagttaccgc ataaacgcat atatatacca
     5041 cacgatctat tagcgcgtgt acgcgtgtag gttatgcggt aactgattag gtacttcccc
     5101 gtgattcaag acgtgactgc acgggaacgc gtcgcattgt taaacacagt ttgtttttgc
     5161 tgtccggttt tttaatcacc catttttgga gaattgtatg agcaagaatt ttaccatcga
     5221 acttcgtaac atcgacgcca tccatcccta cgacaaaaac ccgcgaatca acgacggcgc
     5281 agtggatgcg gtggccgcat ccctgaagga gtttggcttt cgccaaccca ttgtggtcga
     5341 caaagatggc gtcattgtct gcgggcatac ccgctacaag gcggcgctgc agttgggcct
     5401 caagaaaatc cccgtccatg tggcgacgga tttgacgcca gagcagatca gggcctaccg
     5461 catcgccgat aacaaaaccg ccgacctggc cgaatgggat tataacatcc tgcccattga
     5521 actatccgaa cttcaggatg ccggatttga tatggacctg ctggccttcg atgaaaaaga
     5581 gctggtcaaa ctgctggata cggatgtgac ccagggtaat actgatctcg atgcggttcc
     5641 cgaacccccg gatgaggcga ttacccagct tggggatgtt tgggtattgg gcaatcaccg
     5701 cctgatgtgc ggcgactcgt ccagtgaggc tgacctggat accttgctgg acggcaaaac
     5761 aatcgatttg gtcaatatgg acccgccata caacgtcaag gtcgaaccgc ggagcaataa
     5821 tgccattgct gccggattga gcagctttgg caaaggtctc cagcatcatc agtcctttga
     5881 cgtagctcgc gacaagacca aagcacagaa aaccacccca aaactccgtg ccaaggaccg
     5941 acccctggag aatgattttg tctcaaaaga ggatttcgat gaaatgcttc tcgcctggtt
     6001 taataacgcc tcacgcgtcc tgcgcccggg tggttcattc tatatctggg gcggctatgc
     6061 caacctgggc aactatccgg ggccgcttaa agccgcgggc ctgtactttt cacaggggat
     6121 tgtgtgggat aaacagcatc cggtcctgac ccgcaagg
//
LOCUS       DAOTBO010000135         6140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_10803_length_6140_cov_6.243878, whole genome shotgun sequence.
ACCESSION   DAOTBO010000135 DAOTBO010000000
VERSION     DAOTBO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10803_length_6140_cov_6.243878"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..401)
                     /locus_tag="PLE88_11655"
     CDS             complement(255..401)
                     /locus_tag="PLE88_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77161.1"
                     /translation="MAKQTDEQKRRVELIDELLKDYKGPESCWGQSGPFARPLRVEGG
                     KRRA"
     gene            complement(581..1663)
                     /locus_tag="PLE88_11660"
     CDS             complement(581..1663)
                     /locus_tag="PLE88_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOM77162.1"
                     /translation="MKIELKEITVRQLADGYADNAEEGVVGFGGKLDIRPPYQREFIY
                     KDKQRDAVITTLINDFPLNVMYWAVREDGKFEIIDGQQRTISICQYVNGEFSYEKRYF
                     HNLKDDEKEQILNYKLMVYFCSGTDSEKLDWFRTINIAGEKLTDQELRNAVYAGTWVT
                     DAKRYFSKTGCPAYQIASDYLNGSPIRQEYLETAIKWISNDHIEEYMAQHQHDQNAGA
                     LWRYFQDVITWVSTTFTKKRKFMKGVDWGTLYNTYKDKTYDTKKIEEETARLILDDDV
                     TKKSGIYPYILTRDEKYLSIRTFSDAMKQKVYERQKGICEGCKKHFEIEEMEADHITP
                     WREGGKTTEDNCQMLCRECNRRKSGR"
     gene            complement(1660..2655)
                     /locus_tag="PLE88_11665"
     CDS             complement(1660..2655)
                     /locus_tag="PLE88_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HOM77163.1"
                     /translation="MSQKSLNKNLHAAKSAKKDEFYTQLPDIERELKHYTRHFKNKVV
                     LCNCDDPRISNFFHYFSYNFEKLKLKKLITTCYKNQDMDLFSRNNSEKAIYLEYYGDK
                     NGNKVPDPAEIGIKHLKGDGDFRSPECIELLKQADIVVTNPPFSLFREYVAQLMEYDK
                     KFVILGNKNAITYKEIFKLIKENKIWVGNTPMGTDILFDVPEDFAKEVISNKNEGSGY
                     KIINGVVKARAQAVWFTNLDIDKRHEDLILYKKYNPEEYPKYDNYDAINVDKTKDIPA
                     DYDGVMGVPITFLDKYNPDQFEIVKFRKGNDDKDLSINGKYPYFRILIRKKKIKK"
     gene            complement(3208..4439)
                     /locus_tag="PLE88_11670"
                     /pseudo
     CDS             complement(3208..4439)
                     /locus_tag="PLE88_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            4812..5036
                     /locus_tag="PLE88_11675"
     CDS             4812..5036
                     /locus_tag="PLE88_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77164.1"
                     /translation="MASTKSGSVEKPNFKKIRIFETFCGLEYLNSKRGKCISGEYKWR
                     ISIDGILGKIKNNQDKEGDLCANYGFGVWR"
     gene            5027..5704
                     /locus_tag="PLE88_11680"
     CDS             5027..5704
                     /locus_tag="PLE88_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77165.1"
                     /translation="MALILLMSSIAAVSEAAITVYTDKTEWENALSGSFVTEDFSDDQ
                     LNIGVSFVSTESGHINPAEECYQDVLASTSQNEPATTWSFAAEIIGYGGNWTLGGPGG
                     SGNYLLIYIDDLLPYVGFISNSYSGEFWGFISDTPFTSVRLVGGTGTNQQNYRLDDMV
                     YSQYDPLICEIDLNADGIVNLVEFAYIAAEWQTETTPEMGDLTGDGQVNIDDLAELGM
                     FWLRYCL"
BASE COUNT         1573 a         1363 c         1274 g         1930 t
ORIGIN      
        1 acaacaagtc cgcacggaat gtaaataaaa accgcccgga cggcatcaaa atagggggct
       61 tcaaagggac cctgaagctt gtaaagcgtg tttaaaaaag ggcataacac actttgacag
      121 cactatgctg gagcaggatt tttctcctga ataagagccc aactgggcag cggggcttgc
      181 tgtctttact gccggctgct gcaatacaat tatgaccgcg gacacgatgc cttgggcggc
      241 cagagtcccg ttgattatgc ccgccgtttt cccccttcga cccgcagcgg ccgggcaaat
      301 ggccccgatt gaccccaaca cgactccggt cccttgtagt ccttgagtaa ttcgtcaatt
      361 aattcaacac gccgtttctg ttcatctgtc tgcttggcca tagtaagtcc cttttccaat
      421 taaggttaac atatctatga ccatttacac agtttatttt acagcctcaa atgtcactaa
      481 attagtgcaa aatcgtttat aattaccaaa aattatattc ctttcacatc gttatccatt
      541 gtctttggtt tgttctttga gccattgcat tgctgttgct tcatcttccc gattttcttc
      601 tgttgcactc tctgcacagc atttggcaat tatcttcggt tgtttttccg ccttcgcgcc
      661 agggtgtaat atggtcggct tccatttctt caatctcaaa gtgtttttta catccttcgc
      721 aaatcccctt ttgtctttca tagacctttt gtttcattgc atcggaaaag gtacgaatag
      781 aaagatattt ttcatcacgg gtcaggatat atgggtagat accgctcttt tttgttacat
      841 catcgtcaag aataagtctt gccgtttctt cttcaatttt ttttgtgtca tatgttttgt
      901 ctttgtaagt attataaagt gttccccaat ccacgccttt cataaatttt cttttttttg
      961 tgaacgtcgt gcttacccaa gtaatcacgt cctgaaaata tcgccacagc gcaccggcgt
     1021 tttggtcatg ctgatgctgc gccatatatt cttcaatatg gtcattgctg atccatttaa
     1081 tagctgtctc cagatattcc tgacgaatgg gtgaaccatt aagataatcg cttgcgattt
     1141 gataagcggg gcaaccggtt ttgctgaaat agcgttttgc atcggtaacc catgtaccgg
     1201 cataaacggc atttcgcagt tcctgatcgg taagtttttc gccggcgatg ttgatggttc
     1261 taaaccaatc cagcttttcg ctatctgtgc cgctgcaaaa atagaccatc agtttatagt
     1321 ttaggatttg ctctttttcg tcatctttga gattgtgaaa gtatcttttt tcataagaga
     1381 actctccgtt gacatattga caaatggaaa tggttcgctg ctggccgtct ataatttcaa
     1441 atttgccatc ttcccgaacc gcccagtaca tcacattcag cggaaaatcg tttatcaggg
     1501 tagtgataac ggcgtctctt tgtttatcct tgtagataaa ttctctctgg tagggggggc
     1561 gaatatccag tttgccgccg aagccgacaa cgccctcttc ggcgttgtct gcataaccat
     1621 ccgccaactg gcggactgta atttctttaa gttctatttt catttcttaa tcttcttctt
     1681 ccgtattaaa attctaaaat atggatattt accattaatt gataaatcct tatcatcatt
     1741 tccttttcta aattttacaa tttcaaattg gtcggggttg tatttatcaa ggaatgtaat
     1801 cggcacaccc ataaccccgt catagtcggc ggggatatcc tttgtcttat cgacatttat
     1861 tgcatcatag ttatcgtatt ttggatattc ttccggattg tattttttat agagtattaa
     1921 atcctcgtgt cgtttatcaa tatctaaatt cgtaaaccaa acggcttgcg ctcttgcttt
     1981 aacgacacca tttattattt tatatccact gccttcattt ttattcgaaa tcacttcttt
     2041 tgcaaaatct tccggcacat cgaacaaaat atcagtaccc atgggagtgt ttccaaccca
     2101 gattttattc tctttaatca acttgaaaat ttctttgtag gtaatcgcgt ttttgtttcc
     2161 aaggatcaca aattttttat cgtattccat caactgtgcg acgtattctc taaatagtga
     2221 aaagggcggg ttggtgacaa cgatgtcggc ctgttttaag agttcgatac actctgggct
     2281 tcggaaatcg ccgtctcctt ttaggtgctt aattccgatt tctgcgggat ctgggacttt
     2341 gttgccattt ttatcgccgt aatattccag atagatggct ttttcggaat tattgcggct
     2401 gaataaatcc atatcctgat ttttgtagca ggtggtaatg agttttttga gtttgagttt
     2461 ttcgaaattg tatgaaaaat aatggaagaa gttgctgatt cttgggtcgt cacagttgca
     2521 aagaaccact ttatttttga aatggcgggt atagtgtttt agctcccttt caatatcagg
     2581 tagctgggtg taaaattcat cctttttggc gctcttggct gcgtggagat ttttatttaa
     2641 tgatttttgg ctcatctttt tgtctgttta gcaattttta acctaactgc cttaaaaaca
     2701 acgactgaac tatcgttttg tccatttaca acgcctcatt gtaagctaac aatgccgtat
     2761 tgtaagtaga caatggctca atgtaacctt acaatgccat aatgtaaagc cgcaatgcct
     2821 caatgtaagc cgacaatatc acgatgcaac cttacaaagc tacattgtaa accaacatcg
     2881 ccgcgatgta agctgacaat gcgatgatgt aaggcaacaa ggccacgatg taaacttaca
     2941 aaaccgccat cacggggtct gttcatttaa ctgtgtaaat ggccttaacg gtttcacttc
     3001 acaattgaca ttataacccc ttcgcattaa tttgtccata tcgttctgaa ggatagggct
     3061 ctgctggaga gcaacctgag cgtcaggacg atgccgagac aggaagctcc cgcaggtttt
     3121 atggatgccg gagctttttt tatttctgtt ttggcccata cgcaaaaaac tcataaaaca
     3181 gttccgccga aggcggagtc ctttttccta gacctcaaat cgtccggcaa accggatggc
     3241 aaactgattc aatgcagcct tccatgtgtg aattggcatc gtccattttt ttgcgatgtg
     3301 gctcagggcc aggtaaaaaa tcttcaggat ggacgcatcg ttggggaaag cccccttggt
     3361 cttgataatt ttacgcaggg aacgattcag cgattcgatg gcattggtcg tataaatgac
     3421 tttgcggatg tcttcaggat agtcgaagaa ggggaacact cgctgccagt tccttcgcca
     3481 catctggctg atggtcgggt acttcgcatc ccacttcctg ccaaaagcat ccagagccat
     3541 ctcggcctct tgctcagtcg ccgaggtata tatgctcttt agatcttcgc agataagctt
     3601 gcggtctttc cagttgacat atttgaccga attgcgaacc agatgcacga tgcaaagctg
     3661 gacctgggta tcgggaaaga cgctttcaat cgcctcggga aagcccttta aaccatccac
     3721 acaggcaatg aagatatcct tgatcccgcg gtttttaagc tcggtaataa cccccagcca
     3781 gaacttggca ccttcagtct gggctatcca gatgcccaga agttctttct gaccctccat
     3841 cgtcacacct aacgccagat atacactgcg gttacaaacc cggccttctt cacggctctt
     3901 gacgacaatg caatccagaa acaggatggg gtaaatgtta tccaggggac gattctgcca
     3961 ggctttcacc tcctccagta tcccttcggt tgcctgactg ataagaccct cggagacatc
     4021 gatctgatac atatccaaaa gggaagactg gatatcccgg acactcatgc cgcgaccata
     4081 catggccaga atcttatcat caaatccctg aaaatacttc tctcgcttgc ggagcaactg
     4141 gggttcaaac gtgccgttgc gatcccgggg ggctccagtt cgatctggtc agaatccacg
     4201 acgacagtct ttttacccct gccgttgcgg gaattgccgc tgtttttacc cttgggatcg
     4261 tgtttggtat agcccagatg gctatccatc tcggcattca aggtgcgatc aataatccgc
     4321 ttcttaagct gggcaaatag ccctgattga ccccaaaacg actccggtcc cttgtagtcc
     4381 ttgagtaatt cgtcaattaa ttcatcacgc cgtttctgtt catctgtctg cttggccata
     4441 gtaagtccct tttccaatta aggttaacat atctatgacc atttacacag tttattttac
     4501 agcctcttat gcgatttcgg gttcttttta atctcttaca acgtatttcg tgaagcggta
     4561 taaagttcca tccccattcg aataaaccga taccgaaatg tcgatatttc catgtttttt
     4621 tgtcactgta ttctccccaa aacgaccccg gttttgaaac ttgaaattct ccgtactgct
     4681 ttgccccttt tcaatatgtt tatttgcata tctcataact gatcctttcg atctccgttt
     4741 ggttatatac tcttatttga actatacatg ccatttgtta taatagtgtg tcccatttgt
     4801 tagaatatag tttggcaagc acaaaatcgg gttcggttga aaaaccaaat tttaagaaaa
     4861 taagaatttt tgaaactttt tgcggtttgg aataccttaa cagtaagcgg ggaaaatgta
     4921 tttctgggga gtataaatgg agaatatcaa ttgatgggat tttgggtaag atcaaaaata
     4981 atcaagataa ggaaggtgat ttatgtgcaa attacggatt tggggtgtgg cgttaatact
     5041 attaatgtcc tcgatagccg cggtaagtga agcggcgatt acagtttaca ccgacaagac
     5101 agaatgggaa aatgcactga gcgggtcatt tgtaacggag gatttttctg acgaccaact
     5161 gaacattgga gtaagttttg tgtcaaccga gtcaggtcat atcaaccctg ccgaggaatg
     5221 ctatcaggat gtcttggcat cgaccagtca gaatgaaccg gcaaccacat ggagttttgc
     5281 cgctgagatt atcggatatg ggggaaattg gacgttaggc ggccccggcg gttctggaaa
     5341 ctatttgctg atttacatcg acgacttgtt accttatgtg ggtttcattt ccaacagtta
     5401 cagtggtgaa ttctggggat tcatctcaga tacgcccttc acctcagtgc ggttagtcgg
     5461 aggaacgggc accaatcagc aaaattatcg tttggacgac atggtttact cccaatatga
     5521 tccgctcata tgcgagatcg accttaatgc ggatggaatt gtaaaccttg tagagttcgc
     5581 gtatattgcg gcggagtggc agaccgagac tacacctgaa atgggggact tgaccggcga
     5641 tggacaggtt aatatagacg accttgccga attggggatg ttctggctga gatattgtct
     5701 gtaataaatt gaagaggaaa gaaaaattca aattaaagat cttttgtgta tgcaaagtca
     5761 aaattatagg gttctatttc aatagttttt taaaaaaagc ccacctcaaa tcttttaaaa
     5821 gtccttttgc cttttacgct ggacaaccgc tggacccttt tttttattct gattttcctt
     5881 cttgacaaat gcattaaaaa atagtgtatc tatttgcccg ctaaagactt atgttattta
     5941 gggcggttgg ctcagctggt tagagcgcct gccttacaaa caggagggca caatttggaa
     6001 tcctgtaccg cccattctaa aacctcgttt tcaatcacaa aaataattgt ctatggtctt
     6061 attcaagcaa cccataaccg gacaaactgc atcggcagcg aggggcgcaa agtctatatc
     6121 gctttacttg cagttcagcg
//
LOCUS       DAOTBO010000136         5852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_11474_length_5852_cov_4.186303, whole genome shotgun sequence.
ACCESSION   DAOTBO010000136 DAOTBO010000000
VERSION     DAOTBO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11474_length_5852_cov_4.186303"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLE88_11685"
     CDS             complement(<1..363)
                     /locus_tag="PLE88_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77166.1"
                     /translation="MMEYIGLSGKSGAGKTTLAVALRDAYQPERAVVWGFGHEVKIEF
                     ILQYPDLAYSVEDLDSQHIKNIRLSKTQTIRDALIRIGLERRRKDPDYWLRRWLKRTK
                     QMQQCGIHRVIAPDVRFEN"
     gene            complement(421..1083)
                     /locus_tag="PLE88_11690"
     CDS             complement(421..1083)
                     /locus_tag="PLE88_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77167.1"
                     /translation="MMDGKCWRIVLNQKDEWCTATGCAACQAAGYTEGNIKGATMEAF
                     TDDEIKKLKWLVNNLLDRELVNCLLGLKSQQQAKPAAKRFVKPSIEEVEAYIRELGGG
                     FSAQEFYDFYESKGWVVGKSPMKDWRAAVRTWQKRKPPAPQKPADPRRRCTCGNLALP
                     HIRDKNICAICYAKAMQAEASQNSPSISFKEYINGVLAHKNACASSQQTAGCNPGSRA
                     EC"
     gene            complement(1080..1457)
                     /locus_tag="PLE88_11695"
     CDS             complement(1080..1457)
                     /locus_tag="PLE88_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77168.1"
                     /translation="MKNQTTDKNLSAVLRDGLALEYVKEQTPEICLAAVRQNKCAVDL
                     IRDKDMAKKFNGKMDHKHIKYCLAGLLALCLGCSPAVKLYPITGEHIIEAHKGQSFTP
                     PRDGYFLSDDYFTKILKARVEKL"
     gene            complement(1535..1747)
                     /locus_tag="PLE88_11700"
     CDS             complement(1535..1747)
                     /locus_tag="PLE88_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77169.1"
                     /translation="MIKAIAQIAAAIAGIASVLVPLIKWWLRSQNKKIKQQEAAVKEL
                     ESMIKEGSSVSVADMQSRIDDLLNGL"
     gene            complement(1744..2040)
                     /locus_tag="PLE88_11705"
     CDS             complement(1744..2040)
                     /locus_tag="PLE88_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77170.1"
                     /translation="MMENAKEWYKSKTVWGAILALAVAVLTLVFAVSEQAAQNDAMQR
                     LMAVLQDPQTQDAVQKIGDAMSADNSTQKIIGVLGVVGSLIAIYGRITAKDVLK"
     gene            complement(2012..2257)
                     /locus_tag="PLE88_11710"
     CDS             complement(2012..2257)
                     /locus_tag="PLE88_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77171.1"
                     /translation="MSRDNNKELRHIQYCGFRALWVVGLRRLNAEGRYAIHDYCELLA
                     VCIVLTSDERYADVKKKNMNIIQKWKGLYDGKRQRVV"
     gene            complement(2254..2664)
                     /locus_tag="PLE88_11715"
     CDS             complement(2254..2664)
                     /locus_tag="PLE88_11715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOM77172.1"
                     /translation="MAKVEKSRRNRKGYPKYLDNLARTLLRLQCGDKCQRCNKPVDGR
                     DSQMHHIVSRKYYSTRWEMCNLVLLCAACHRRYHDGLFGKDWFAVKFPKKNNELTAKV
                     ASGQQRTTWRTSDFEAVEARLLQELSEEKQRSLA"
     gene            complement(2651..3106)
                     /locus_tag="PLE88_11720"
     CDS             complement(2651..3106)
                     /locus_tag="PLE88_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOM77173.1"
                     /translation="MTETKTTNKTLPIHILPLLLHAAGCFVSFPAGTAALAAAPQDIN
                     RLIDAMCIVESGGMAHAVGDSGSAVGILQIHPVVVRDVNRILGREAFLLRDRLDPAKS
                     RMMCWVYLSHYGGSIEQMARKWNGGPNGHKKPATLAYWRKVKRVLYGEG"
     gene            complement(3103..3432)
                     /locus_tag="PLE88_11725"
     CDS             complement(3103..3432)
                     /locus_tag="PLE88_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77174.1"
                     /translation="MKEPVKIAEMIEKAIAALGEEGRKSEGLIEAKAKSMAEYDKQFA
                     VACAKLKANGVPVGMIDRLAKGETAEILYARIVAEEALRAHYARMEQLQAQLNGLQSL
                     NRYLSVL"
     gene            complement(3429..3968)
                     /locus_tag="PLE88_11730"
     CDS             complement(3429..3968)
                     /locus_tag="PLE88_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77175.1"
                     /translation="MGNFASVPSNGEKIEIKAVLSEIGPMSKNINGKVAQKCKLKDAS
                     GVTKTVTIYAGNSGPLQPELLGSTCVFMVKRFDGKYGPIYQGFCNGVADEPIKPAQTE
                     NGGCDCQASAAAASDDVLRTRSMALKYANDLAVAGVLEPNMVIDVAIVYTQYILNGSV
                     AEKPAQVEQEEVEEEEIPF"
     gene            complement(3961..4725)
                     /locus_tag="PLE88_11735"
     CDS             complement(3961..4725)
                     /locus_tag="PLE88_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77176.1"
                     /translation="MHNERISVTTHTAGRCLVYPETPYGIQPSVTSIISILDKPQLKD
                     WAANCAVDWLINNPQDFDNARKAHKLVSDAAKDTGTDIHALCSRWLLGESIDEDSLET
                     QTTTLLNAFKEWCLVNTIEPVYSEVIIHGPGYSGTCDLIAYRTDPKTQVRRLGLYDIK
                     TAKGSYYPEWGLQLAAYSNAAEDMAADGALELNDSIEELGIIKLNKETLKCNYKEFTS
                     QRDRLFAAFIALKDFYWLYNDLESQIQQLTKETEHG"
     gene            complement(4715..>4951)
                     /locus_tag="PLE88_11740"
     CDS             complement(4715..>4951)
                     /locus_tag="PLE88_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77177.1"
                     /translation="YLCRWCAEHKDEVIESLAASRYSIEKDYRWLLDAVRDCSQWVKD
                     DVAKHYMLRCLAILDMPSGVSKALAKMKDVYNAQ"
     assembly_gap    4952..4961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5004..5168)
                     /locus_tag="PLE88_11745"
     CDS             complement(5004..5168)
                     /locus_tag="PLE88_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77178.1"
                     /translation="MKKESPKRFKVFDTDKIWDDLRAAQELEDLGIDWQALKINPARI
                     TEKEKGKWTK"
BASE COUNT         1190 a         1592 c         1420 g         1640 t
ORIGIN      
        1 attctcaaac cgcacatccg gtgcaatcac cctgtgtatg ccgcactgct gcatctgctt
       61 ggttcgcttc agccaccgcc gcagccagta atcggggtct ttgcggcggc gctccaagcc
      121 gatacggata agggcatcac ggatggtttg tgttttgctt aggcgtatgt ttttgatgtg
      181 ctggctgtcg aggtcttcga cagaatacgc caggtctggg tactgcagta taaactctat
      241 cttgacctca tggccgaacc cccacaccac cgcccgctct ggctggtaag catcacgcag
      301 ggcgaccgcc aatgttgtct tgccggctcc gctcttaccg gacaatccga tgtattccat
      361 catgcttcct tgtcgtcttc gattcgccgc agaacatcct gcatttcttc gatttcaacc
      421 tcagcactcc gcacgtgatc ctggattaca tccagcggtc tgctgagaag atgcacaagc
      481 gtttttgtgt gcaaggactc cattgatgta ctccttaaag ctgattgacg ggctgttttg
      541 ggatgcctct gcctgcatgg ctttggcgta gcatatcgca cagatgtttt tgtctcgaat
      601 gtgcggcagt gcaaggttgc cgcatgtgca ccgccgacgg gggtctgccg gcttctgcgg
      661 agccggcggc ttgcgcttct gccaagtccg aacggctgca cgccagtcct tcatcgggct
      721 cttgccgaca acccagccct ttgactcgta gaaatcgtag aattcttgag ccgaaaagcc
      781 gccgccgagc tcccgaatat aagcctcaac ctcctcaatc gaaggcttta caaatcgctt
      841 tgctgccggc tttgcctgct gctggctctt gagcccaagc aggcagttga ccagttctcg
      901 gtcaagcaga ttgttgacca gccatttaag cttcttgatt tcgtcgtccg tgaacgcctc
      961 catggtcgct cctttgatat tgccttctgt gtaaccggcg gcctggcatg ctgcacagcc
     1021 ggtggctgtg caccactcgt ccttttggtt cagcacaatc cgccagcatt tgccatccat
     1081 cataacttct caaccctggc tttgagtatc ttcgtgaaat agtcatcgct caagaaatat
     1141 ccatccctgg gcggggtaaa agattggccc ttgtgggcct cgatgatgtg ctctccggta
     1201 atggggtata atttaactgc agggctgcag ccaaggcaca gggcaagcag gccagcaaga
     1261 caatatttga tgtgtttgtg atccatcttt ccattgaatt ttttcgccat atccttatct
     1321 cggattaggt caacagcaca tttgttttgc cggacagcgg ccagacatat ttcaggggtc
     1381 tgttccttga catactccag tgccagtccg tcccgaagca cggcggaaag gtttttgtct
     1441 gttgtttgat tcttcatttt gcccgctccc tttccaggtt ttataaagac ccgccggcaa
     1501 cgcaccggcg ggcggaagtg agaagaacag aaaattataa tccgttcagt aaatcatcta
     1561 tcctgctctg catatcagca acactaacac tggagccttc cttaatcata gactccagct
     1621 ccttgactgc cgcctcctgc tgtttgattt ttttgttttg gcttcgcagc caccacttaa
     1681 tcaggggaac aagcacggat gcaatgccgg caatggcagc ggcaatttgg gcaatggctt
     1741 ttatcatttc aggacatcct ttgctgtgat gcggccataa atggcgatga gagagcccac
     1801 cacccccagc acgccaataa tcttctgcgt gctgttgtcg gctgacattg catcgccaat
     1861 cttctgaacg gcatcctgcg tctgtgggtc ctgcagcaca gccataagcc tctgcattgc
     1921 atcgttttgg gctgcctgtt cgctgaccgc aaacaccagc gtcagcacgg caacagcaag
     1981 agccagtatt gcgccccaga cagtctttga cttataccac tctttggcgt tttccatcat
     2041 aaagtccttt ccatttttga ataatgttca tatttttctt tttaacatct gcgtatcttt
     2101 catcactggt tagcacaatg catactgcca gcagctcgca ataatcgtgt atagcatacc
     2161 tgccttcagc attgagcctg cgaaggccaa cgacccacag cgcccgaaag ccgcaatact
     2221 ggatatgccg aagctctttg ttattgtccc tgctcacgcc agactccttt gcttttcttc
     2281 actgagttcc tgcagcagcc ttgcctcgac tgcctcaaag tcgctggtgc gccaagtagt
     2341 cctttgctgc cccgatgcaa ctttggctgt taattcattg ttttttttag ggaattttac
     2401 tgcaaaccag tccttaccaa acaagccgtc gtgataccgc ctgtgacaag ctgcacacaa
     2461 cagcaccaga ttgcacatct cccaccgtgt gctgtaatac ttgcggctga caatgtgatg
     2521 catctggctg tcccgcccat ccacaggctt gttgcagcgc tggcatttgt cgccgcactg
     2581 gagtctaagc agtgtgcggg cgaggttgtc gaggtatttc gggtatccct tgcggtttct
     2641 acgggacttc tcaaccttcg ccatacagca ccctcttgac cttccgccag taagccagcg
     2701 ttgccggctt cttgtgcccg ttcggcccgc cgttccactt gcgggccatc tgctctatgc
     2761 tgccgccgta atgcgacagg tagacccagc acatcatcct tgacttggct gggtccagcc
     2821 tgtcccgcag caagaacgct tccctgccca gtatgcggtt gacatccctg acaaccactg
     2881 ggtgtatttg gagtattcca acggccgatc cgctgtcgcc aacggcatgt gccatgccgc
     2941 cgctctcgac tatacacatg gcgtcaatca gccggttaat gtcttgcggc gctgctgcca
     3001 gcgccgccgt gccggctgga aaggagacga aacagccggc tgcatggagg aggagtggaa
     3061 gaatatggat tgggagagtt ttgtttgttg tttttgtttc cgtcatagca cactcaagta
     3121 tctgttcaaa ctctgcaggc cgtttaactg ggcctgcagc tgctccattc ttgcgtaatg
     3181 cgcccgcagg gcctcttcgg cgacaatgcg ggcatacagt atctctgccg tttcaccctt
     3241 agccagccgg tcaatcatgc cgaccggcac gccgttagcc ttaagtttcg cacaagccac
     3301 ggcgaactgc ttatcatact ctgccattga cttggccttg gcctctatca ggccctcgga
     3361 ctttcggccc tcttcgccaa gggcggcgat ggccttttca atcatctcgg ctatcttcac
     3421 gggctctttc aaaagggaat ctcctcttct tcgacttctt cctgctctac ctgtgcgggc
     3481 ttttccgcca ccgagccgtt cagtatgtac tgcgtgtaca caatagccac gtcgataacc
     3541 atgttgggct caagcacacc agctacagca aggtcgtttg cgtacttcaa cgccatcgag
     3601 cgggtacgca gcacatcgtc agatgcggca gcagctgaag cctggcagtc acagccgccg
     3661 ttttcggtct gggcgggctt gatgggctca tccgcaacgc cgttgcagaa gccctggtaa
     3721 atgggcccat acttgccatc aaaccgcttg accataaaca cgcaagtgct gccgagaagc
     3781 tctggctgaa ggggtccgga gttgccggca taaatggtga cggttttggt cacgccagac
     3841 gcgtctttca gcttacactt ctgggcaacc ttgccgttga tgtttttgga catcggccca
     3901 atttcagaca agacagcttt gatttcaatt ttttcaccat tacttggcac gcttgcgaaa
     3961 ttacccatgt tcggtctcct ttgttaattg ttgaatctgt gattcaaggt cgttataaag
     4021 ccaataaaaa tccttcaggg caatgaatgc ggcaaacagc cggtcacgct ggctggtgaa
     4081 ctccttgtag ttgcacttga gggtttcctt gttcagcttg atgataccca gctcctcaat
     4141 tgaatcatta agttccagtg ctccatctgc cgccatgtcc tcggccgcat ttgaatatgc
     4201 ggccagctgt agaccccact cggggtaata actgcctttg gccgtcttga tatcatataa
     4261 gcccagcctg cgaacttgtg tcttagggtc tgtgcggtat gcaatgaggt cacaggtgcc
     4321 ggagtagcct gggccgtgga tgatgacctc ggagtacaca ggttcaatgg tgttcacaag
     4381 gcaccactct ttaaatgcat tcaaaagagt ggtggtttgt gtttcaagcg aatcctcgtc
     4441 aatggactcc ccaagcagcc accttgaaca caaagcatgt atatctgtgc ctgtatcttt
     4501 ggctgcatcg gataccagct tgtgggcctt gcgggcgtta tcaaagtcct ggggattgtt
     4561 tatcagccaa tccacggcac aattggccgc ccaatccttc agctggggct tgtccaggat
     4621 gcttatgata gatgtcacag acggctggat gccgtatggc gtctcggggt ataccaggca
     4681 tcggccggcc gtgtgagtgg tgacggatat tctctcattg tgcattgtaa acatccttca
     4741 tcttggccaa ggccttgctg acgccggagg gcatgtctaa gattgccaag catcgaagca
     4801 tataatgctt ggctacatcg tcttttaccc actggctgca gtcacgtacg gcatccagca
     4861 gccaacggta gtccttctca atactgtatc ggcttgcagc caaagactca atcacttcat
     4921 ctttgtgctc agcacaccac cggcacaggt annnnnnnnn ntggtgtagg agcactcctc
     4981 taattcttcg cagcattcgc attttatttt gtccatttgc ctttctcctt ttccgtaatc
     5041 ctggcggggt taatcttcag ggcctgccag tcaatgccca agtcttcaag ctcctgggca
     5101 gccctgaggt cgtcccagat tttgtctgtg tcaaacactt tgaaccgctt gggactctct
     5161 ttcttcatgg ttctcctcag tgataaacat tgattggtgt tccgcagcct gtgcccttcc
     5221 gcattacctt gtcaatcacc tcggcgttaa acctcacgct tcggccgatg cggacgtgtg
     5281 ggagcacgcc gaggttagca agcttgtata tttgggctga cgaaaccgca agcttctgtg
     5341 cgacttctct cggctttaat aattttgttt ctcttgtttc catgttgttt ttcctgtttc
     5401 tattctataa tatattatag tataaattaa acaataaaac aagtaaaaat cctttgtaaa
     5461 tataaaattt ttaccaaaat ctttgttatt ttatcaaaaa cggccttttt ggtggtattt
     5521 atttttgtgt tggttttgat ggtggacact tggcatatga tacttttggg ggcttgaggg
     5581 catttggtgt atgcagcgga taccctggga gtgccttttt tactgtttgt tttttatggg
     5641 tcaagtgttt ttttattttt tttgtgtttt ttttgcgcgt ctttgggggc tatccatttc
     5701 acggcgtcgc aggccagcgg ccgggcagtg ccggatttgt accggcacag ataagtgtgc
     5761 agtgtaattt tgaagtgtga gtaggcgtgc cggacgatgt tagtttcagc aagccgcatg
     5821 agcaattacc ttgggcagtt catcccaggt tt
//
LOCUS       DAOTBO010000137         5828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_11526_length_5828_cov_4.757838, whole genome shotgun sequence.
ACCESSION   DAOTBO010000137 DAOTBO010000000
VERSION     DAOTBO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11526_length_5828_cov_4.757838"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..951
                     /locus_tag="PLE88_11750"
     CDS             163..951
                     /locus_tag="PLE88_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOM77179.1"
                     /translation="MDTTRAAWDLRRASLSCIRRHHYEVAVLPVGAVEAHNRHLPEGM
                     DVIHSEYVALTSCQKAWPHCQSVICLPAVPFGVDCNLMAFPLTVHVSQKVLDAYITEI
                     LLSLRKHGIRKVVIINGHGGNDFKPLARQIQYETDIYLFLCDWWKVGSDIYEEWFGKG
                     DDHAGAMETSVAMEICPELVDPENAGDGRVPPFRFEAIQKGWITTSRDFSKLNDHCAV
                     SSDFPPACQKGKQYLALVCDRISRFLAELANSKIDSAFPMQPQQ"
     gene            1520..2305
                     /locus_tag="PLE88_11755"
     CDS             1520..2305
                     /locus_tag="PLE88_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOM77180.1"
                     /translation="MEQISETILRERAIPLRHVFDFIPLHSNPRKGALLSEDAYEHIL
                     AHIVFPQDNNSSGIQYGGKITESKIANVLQMSNGPVREAIYRLRQEGWIQTIGNRGSF
                     FVDFSDPAIASQIYQFRLSFETGAFYTLAAKITDAQLNQLYSILTALEQAKQNSDMVE
                     FRKSDILFHLQVVEFAGGEHYKQIFRSKLLQWYAMSYQLLVRAMGKENYSHTLEAPGT
                     PTHRELYEALAGHNSALAAERISKHFTFIPHLLSSNQSTAAEK"
     gene            2414..4432
                     /locus_tag="PLE88_11760"
     CDS             2414..4432
                     /locus_tag="PLE88_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77181.1"
                     /translation="MMKKYQWAAMVTLAVLLGFFQSAAIAIEFTESYGNFINNNAAGY
                     AFVWKTGQATLTGAPSPLPAPMYLRNWTYALSSTGHGCSDGMYLAVMTWDGNLANSTV
                     VGISSSYVDMAAEYAAWRTEEGSNTGFGTTWYTGFRTVTWQFDYVPVQPNTLYAMTFM
                     RKNADGNFYISLGGVELDTSNAFTGGFVTGGTTMNQPAWEAHYIATYSDVTPYPTYTA
                     PADGAVNQATNVQLSWTAPNAFIPVGYNVYIDPFEPNLTPAEADYYVANQPGTTYTPT
                     PALTNGTTYYWRVEALEPNLIAPYAPIPHSGPVWEFTTVPLAVISVADPVSQTVAAGD
                     TVVLTFETLNATSWEWYKNGSILAGETSPTLTIPNFQLANEGFYTCTASNTLPSSVTS
                     SAAQVMTKRLAGWWKLDDPDTTGGYLADSVQEEVPGAPAHIATAAEYRVAAGKDGSAI
                     ELNGTTTCLVTVSNSADFFNFYPQGYTVSAWINRSAASAWGAYVSKQSPATTEPAAPA
                     KGFILTHDNVGQPVHTLRTVSPNDLSSNIRADDNLWHLVTGTYDAATKTSRIYVDGIL
                     RNQVTYSAHPAVSPAALIFGAEFPNGAVAYTGLLDDVRIWTYPLNAQEIAKLYTDFNP
                     SVYICVEQPALDITGPNGVPDCKVDLYDFAQTAAAWLECGRYPELSCN"
     gene            4486..>5828
                     /locus_tag="PLE88_11765"
     CDS             4486..>5828
                     /locus_tag="PLE88_11765"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77182.1"
                     /translation="MRKTVFLMIAAIITTTVNAEIIYLDNFESYAVANPAGADFLANW
                     TVSGPGGANSSRIFQTNNFGGTRLWISQVDGTSITSSGIAIASNNTYRFSAMLASETS
                     DGTRKLNVTYDLLAGQNAAEATSIIGGPVAVVTAGDNYLVPASKVDHIFTRDFTTGTL
                     NPGDKLFIQITLVGVNSGSKPFFGVDDVKIVRLAEPELVSPANGAAHQAVDVQLNWTA
                     PASYTPVGYNVYIATSEPNLTASAADRYSSAQEGLTYTPSPVLARGTTYYWRVEALEP
                     NAIAPYKPIPHSSSVWSFTTLPASVVITQQPLGQTVPAGTTAVLTVEALNAQTYQWYK
                     NNTLLAGQTGSSLTLANVQLADEGFYHCAVSNNLPSTAVSAAAHVMIQRLVAHWPLDG
                     TLQAIESNPAENWDGVYMNVAEPPATPTVSTEPVVYVTGANGVPGSAVRFSGGRVV"
BASE COUNT         1463 a         1490 c         1459 g         1416 t
ORIGIN      
        1 atacggactg cccgactatg ccgaaatccg gaaactgatg ctaaggcaac tgcgggaatt
       61 ggagagaatc tatcaggtgc cgtcggcaca gaaatatgac caaatccgta atccgtaagg
      121 tctggactgc atacgatgga ctttcattaa ggagtatcga ctgtggatac aactcgagct
      181 gcatgggatt taaggcgggc cagtttatcc tgcatccgcc ggcatcatta cgaagtggct
      241 gtgctgccgg tgggagcagt ggaagcccat aatcggcatt tgccggaagg aatggatgtc
      301 atccacagcg aatatgttgc gctgacatcg tgtcaaaagg cttggccgca ctgtcaatcc
      361 gttatttgcc ttcccgctgt ccccttcggc gtggattgca acctgatggc ctttccgctt
      421 actgtacatg tttcacagaa ggttttggat gcgtatatta ccgagatcct attatcgctt
      481 cgcaaacacg gcattcgaaa agtcgtgatc attaatggcc acggcggcaa tgattttaag
      541 ccgctggcca ggcagattca gtacgaaaca gacatctatc tgtttctgtg tgactggtgg
      601 aaagtcggtt cggatatcta cgaggaatgg tttgggaaag gcgatgatca cgccggcgcg
      661 atggagacat cggttgcaat ggagatttgt ccggaactgg tcgaccctga aaacgccggc
      721 gacgggcggg ttcccccctt ccggtttgaa gcaatacaaa aaggctggat tacaaccagc
      781 cgtgactttt caaagctcaa tgaccattgc gccgtaagca gcgattttcc ccccgcttgc
      841 caaaaaggaa agcagtacct tgcattggta tgcgatcgca tcagtcggtt tcttgcagaa
      901 ttggccaaca gcaaaatcga ttctgctttc cctatgcagc cgcaacaata atgcccctgc
      961 aaatacttag ggatggtgat tgtctggccg ctgcagagtt atcagcggga caacaaacca
     1021 tttcctacag cagtcaggac atttcagatt cctgacatcc tgattgttat ggtccattat
     1081 ttcgaacgca tagtcttggt ttcccatcgg tatgcaacac atcaggctcg gcatcgtcgt
     1141 cgctgccttt tgttgttaat gcatttgata agtcctataa catccttaag aatagacaga
     1201 tttcttgcaa tgccgtattt ttatgcgact ctaaagtccc cgcaacttaa tccttgttca
     1261 cggattgcca tccccccttt gggattagaa aaagtatctc actaaatatc tgcaaaatgt
     1321 gtttttgcat gtttttgatg ccctttttgg gtgtaatctt cttttgtatt tttcataagt
     1381 acatataaat aaatggttta taataagata aaatttttaa aaatttcgtt tgacatcctt
     1441 tccacaatac cttattatat cacttaagca cttaagtgct taagcactga tactaatcgc
     1501 attagatcgg gagaacattg tggaacagat atcagagacg atattacggg aaagggcaat
     1561 ccccttgcgg catgtttttg actttattcc acttcacagc aatccacgca aaggagcact
     1621 gctttccgaa gatgcctatg aacatattct ggctcatatt gtatttcccc aagacaataa
     1681 cagttcgggc atccaatacg gcgggaaaat cacggagtcc aaaattgcca atgtccttca
     1741 aatgagcaac ggtccggtaa gagaggcgat ttaccgtctg cgtcaggaag ggtggattca
     1801 aaccatcggc aatcgaggat ctttttttgt cgatttcagc gatccagcga ttgccagtca
     1861 aatttatcaa tttcggctgt cattcgaaac cggagcattc tatactctgg cggccaagat
     1921 tactgatgcg cagcttaacc aactgtattc cattctaacg gcactcgaac aagccaagca
     1981 aaactcggat atggtggaat tccgaaaatc cgatattttg tttcatctgc aagtcgtcga
     2041 atttgccggc ggggaacact acaagcagat tttccgctcc aaactgttac agtggtatgc
     2101 gatgtcttat cagctgcttg ttcgggcaat gggcaaagag aattacagcc atacattgga
     2161 agcacccggc acgccgaccc accgtgaatt gtacgaggcg ctggctggcc ataacagcgc
     2221 tctggcagcc gagaggatat caaagcattt cacctttatc cctcatctgc tcagcagcaa
     2281 tcaaagcaca gcggctgaaa agtaaggata ttggcgaagg aaaatggatg cggtattaac
     2341 acatgataaa caaggattca gacagaaaat aacgaatgga ttcattaaca aaacagttta
     2401 ttgggaggtg tctatgatga agaaatatca atgggcagca atggtaacgc tggcagtgct
     2461 tcttggattt ttccaaagtg ccgccatagc cattgagttt accgaaagct atggcaattt
     2521 tatcaacaac aacgcagccg gctacgcgtt cgtctggaag accgggcagg ctaccctgac
     2581 gggtgcccct tcgcctttgc cggcaccgat gtacctgcgg aattggactt atgccctgtc
     2641 atctaccggg cacggctgca gtgacgggat gtatttggct gtaatgacat gggacggcaa
     2701 tttggctaat tcaacagttg tcggcatctc gagcagctat gttgatatgg cggccgagta
     2761 tgctgcttgg cggaccgaag aaggaagcaa taccggtttt ggcacaacat ggtataccgg
     2821 cttccgaaca gtcacctggc agtttgacta tgttcctgtg cagccgaata ccctgtacgc
     2881 catgacattt atgcggaaaa atgccgacgg caatttttac atcagtttag gcggtgtcga
     2941 actggataca tcaaatgctt ttaccggcgg ctttgttact ggcggtacga cgatgaacca
     3001 gcctgcttgg gaagctcatt acatcgccac ctacagtgat gtcaccccct atccgaccta
     3061 cacggccccc gccgacgggg cggtcaatca ggccacgaat gtgcagttaa gctggacggc
     3121 ccccaatgca ttcatcccgg tcggctacaa tgtgtatatc gatccatttg aaccgaacct
     3181 gacccctgcg gaagctgatt attatgtcgc taaccagccg ggaacaactt atacaccgac
     3241 accggccctg accaacggca ctacgtatta ctggcgtgtc gaggcacttg agcccaatct
     3301 gatagctccg tatgcgccga ttcctcattc cggccccgtc tgggagttca cgactgttcc
     3361 tctggctgtc atcagtgttg cggatccggt cagccaaaca gtcgccgccg gtgatacagt
     3421 ggtcctgacc tttgaaaccc tcaatgccac atcgtgggaa tggtataaaa acggttctat
     3481 tctggccggc gaaaccagtc ctaccttaac tatcccgaat tttcagctgg ccaatgaggg
     3541 attttatacc tgtacagcgt ccaatacctt gcccagttca gtaacctcat ccgcggctca
     3601 agtgatgacc aagcgtctgg ccggctggtg gaagctggat gatcccgaca caacaggcgg
     3661 ttatctggcg gattcggtac aggaagaagt ccccggtgct ccggcccata tcgcaaccgc
     3721 agcagagtat cgtgttgcgg ccggcaaaga cggcagtgca attgaactga acggcacgac
     3781 aacctgcttg gtaacggttt ccaacagcgc tgatttcttt aatttctatc cgcagggcta
     3841 cacggtcagc gcgtggatca atcggtctgc ggcctctgca tggggcgcat atgtctccaa
     3901 gcagtcaccg gccacaacgg aaccggccgc ccctgccaag ggctttatcc tgacccatga
     3961 caacgtgggc cagccggttc atacgctgcg caccgtcagc cccaatgatc tttcctcgaa
     4021 tataagggcc gacgacaatc tctggcattt ggtaacaggc acctatgatg cagccaccaa
     4081 aacaagcagg atctatgtgg acggaattct aagaaatcag gtaacctact cagcgcatcc
     4141 ggctgtcagc cctgcggccc tgatattcgg tgccgaattt ccaaacggag ccgttgccta
     4201 cacgggcctg ctggatgatg tccgtatctg gacctatccg cttaatgcgc aggaaattgc
     4261 caaactttat acagatttca atcccagcgt ttatatctgt gtagaacagc cggcattgga
     4321 tattacaggc cccaacggtg tccccgactg caaggtggat ctgtatgatt tcgcgcagac
     4381 agccgcggcg tggctcgaat gcggccggta cccggaactg tcctgtaatt agcttgttgc
     4441 gggattgaaa aaacagttgc cttaacgata gcaggagaaa taaagatgag aaaaacagta
     4501 ttcttaatga tagcagcgat tatcacaaca accgtaaatg ctgaaattat ctacctcgat
     4561 aatttcgaga gttatgcggt ggccaatcct gccggcgccg attttctggc taattggacc
     4621 gtctcgggtc cgggaggggc aaattcatcg cgaattttcc aaaccaacaa tttcggcggc
     4681 acgcgcttat ggatctcaca ggtggatggg acgagcatca ccagcagcgg cattgccatt
     4741 gcaagcaaca atacctatcg gttttcggca atgctggcat ccgagacatc agatggaact
     4801 cgcaaattga atgtaacata cgaccttctg gcaggccaga atgccgcaga ggcgaccagc
     4861 atcatcgggg gaccggttgc agtggttact gccggcgata attatttggt gccggcttcc
     4921 aaggtcgatc atattttcac ccgggatttc acaactggaa cactgaatcc cggcgacaag
     4981 cttttcattc aaattacatt agtcggggtt aactcaggaa gcaaaccttt ctttggcgtc
     5041 gatgatgtga agatagtgcg gctggctgaa ccggagctgg tatcgccggc caatggagca
     5101 gcccatcagg ccgtcgatgt gcagctgaac tggacagctc cggcatctta taccccggtg
     5161 ggctacaatg tgtatatagc gaccagtgag ccgaacctaa cagcatcggc agcggaccgc
     5221 tacagttccg ctcaggaagg cctgacctac acccccagcc cggtgctggc gcggggcacc
     5281 acctattatt ggcgtgttga agcgctggaa ccaaatgcca ttgctcctta taagccgatt
     5341 ccccattcga gctcagtgtg gtcctttaca acactgccgg ccagtgtcgt cattacccag
     5401 cagccactgg ggcagaccgt tccggccggc acaacagcgg tcttgaccgt ggaagccctg
     5461 aatgctcaga cctatcaatg gtataaaaac aatacgctgt tagcaggcca aacaggctcc
     5521 tctctgacat tggcaaacgt ccagttagct gatgagggat tctatcattg tgcggtatcc
     5581 aataatctgc ccagtacggc ggtctcggcg gctgcgcatg tgatgataca gcggctggtg
     5641 gctcactggc ctttggatgg aacactgcag gctattgagt ccaatccggc cgaaaactgg
     5701 gacggcgtct atatgaatgt agcggaaccg ccggcgaccc caacggtgtc cacagaaccg
     5761 gtggtttatg tcaccggcgc aaacggtgtc cccggcagtg cagtccgatt cagcggcgga
     5821 cgtgttgt
//
LOCUS       DAOTBO010000138         5811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_11572_length_5811_cov_5.311501, whole genome shotgun sequence.
ACCESSION   DAOTBO010000138 DAOTBO010000000
VERSION     DAOTBO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11572_length_5811_cov_5.311501"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLE88_11770"
     CDS             <1..774
                     /locus_tag="PLE88_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77183.1"
                     /translation="LGLIFSRGLGKNNSLGRLAMLSRQVDFFFRGFLPSQLVSPPQGH
                     EEFKNIYCVYTGGDDLLLVGPWSTVLEFALFLNNAFRRYTCNNPKVTLSAAVAVIHPN
                     MPLARAAMQADQMLEEAKDAGRNRMAVFNEVMTWDEYAKALDDGLFLHKALTDKGTDG
                     IKINRGFVYRLLNYYKMASDETKVKNLIWRSHLAYDIARNVEQPGVRDSQIDTPGRRR
                     IKEMTRFGIGRKDEIKRLKVSATYCLYLNRKGDKNGACI"
     gene            758..1219
                     /gene="csm2"
                     /locus_tag="PLE88_11775"
     CDS             758..1219
                     /gene="csm2"
                     /locus_tag="PLE88_11775"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOM77184.1"
                     /translation="MAHAFDNRQRPWQHGGGGTPRQAASVPVTFYRDPANKKQLNPTL
                     LNEDAEKQAAALHDKINSAQIRRFFSEVKNLSLRLEQGRPWTQIEPFFRMLRSKAEYA
                     RGTGKIPPDFANFISDNTKPDRVKDEQDFRAFVLYFEAVLGFAYGKGLVKK"
     gene            1216..1884
                     /gene="csm3"
                     /locus_tag="PLE88_11780"
     CDS             1216..1884
                     /gene="csm3"
                     /locus_tag="PLE88_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOM77185.1"
                     /translation="MNWKLINTKRITGKITVITGLHIGGSTELMEIGGVDNPIIKNPV
                     DNSPYIPGSSLKGKMRSLMEWKLGLLSANGKPYTSDRPDCPITRVFGGMAGATKFGPT
                     RLVVRDAFLTAESRKAFDEGQELTEVKHENSIDRITSEANPRPLERVVPGVAFDLDIA
                     YKVIDTDDGGAVDENYFQEVVLRALALVQEDFLGGSGSRGCGRIRFDDLKDEKGNPLQ
                     LPSA"
     gene            1896..2936
                     /locus_tag="PLE88_11785"
     CDS             1896..2936
                     /locus_tag="PLE88_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77186.1"
                     /translation="MKTYKLKLQVYSALQTPLQPDTLFGHLCWSIRYTEGQQALQAFL
                     DSFMQGPPPVVLGSAFPEGFWPAPLLPGPSRQQEDILLDMLSKISRTELKNRLPFCPL
                     SENSVSKTLTDLEAFDILKWLRKLRFLPEEMLPEFAAAMDSFAILQRFLQNGCGKANL
                     LTSAAVAHNSVNRLTGASDELYFSNEYFPNPKKPPVFQLVVMSDIWDAHQITEKFTAA
                     LQGGYGKSKSRGKGWIKVIGTEPFTLPHAQSPNALLLLGTCCPAQTDPAQGFWQCATK
                     AGKLGGDWAVGDNPFKKPLTMLLPGSLLKTDSPKSYCGRLVKGVSDAYPQVVQYGLAP
                     VLAVRWDAKEVL"
     gene            2933..4021
                     /gene="csm5"
                     /locus_tag="PLE88_11790"
     CDS             2933..4021
                     /gene="csm5"
                     /locus_tag="PLE88_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOM77187.1"
                     /translation="MKRYKMTITILSPVHIGSGQQIDPLEYLVKDTIFYRLDTARFLS
                     TLTANLKAKFNEALKSSNPVTMREFIVDNLDTKKYSLFSAATGPDFEKAYANNVDSIN
                     NQLLVELFPRCGTQLRAYLPGSSIKGAIRTAVVSQLAKPGISPAVYTDDRGQHPYKSG
                     TRLEKYILRCKDAKQDPFKMLRIADAFLPDDATFIDLAQIYKRDRAAGAADPGKIQIF
                     CEQCFSMLDNERITAEGMLEINTDLIGKKGVDRRGNSVDAVSMPLTAEQILNSCKAFY
                     LPKMDHEHKVFYDDNPQLRTHSQKLLDVKYADNECPIRIGRFCHVECHTLDNYREPKT
                     RRGWGKTRTLSAGTMPMGWVKMRLDEMK"
     gene            4038..5132
                     /gene="cas1"
                     /locus_tag="PLE88_11795"
     CDS             4038..5132
                     /gene="cas1"
                     /locus_tag="PLE88_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOM77188.1"
                     /translation="MFFDKFIAFSGAGESYLIATVCRYEYGVTLSMQTLYVKEQGAKV
                     GCTGRRLTVTKEEQELLTVPICRLTRVVLIGRVQITTQAAALLLDRRIPLVMATLTGR
                     LRGVLVPPDTPHVTLRLNQYELAKNPQYGFNFCRDLVAARAASACNVIRRYLYNHPKK
                     DLRPHIQKILDYASQVSNQPSIDSLRGMEGMIAREYFAALVKIFQDLGMNFSGRIRRP
                     PTDPVNACLSYTYILLTEHCSCALQTTRLDIYLGFMHQPNRNAPALALDFVEQFRQPV
                     ADRFVMLLFNKKMLQPSDFSPGPMNAVLLSEQAKRTLMTQWEIFLDTPQRLLENQPAL
                     SPRDLLYRQAEAMEAAVRQKQPFCHYRLCL"
     gene            5142..5417
                     /gene="cas2"
                     /locus_tag="PLE88_11800"
     CDS             5142..5417
                     /gene="cas2"
                     /locus_tag="PLE88_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOM77189.1"
                     /translation="MFLVIGYDCTDDKRRLKIAKILLDYGYRVQYSVFEADLDQPLLD
                     ELIKRLAKVIHPGEDSIRIYHICQRCLEQTQLFGDAQLTHQEKVFIV"
BASE COUNT         1399 a         1617 c         1556 g         1239 t
ORIGIN      
        1 cttggactga ttttcagccg ggggctgggc aaaaacaact cgctcggccg gctggcaatg
       61 ctgtcccgcc aagtggattt cttcttccgg ggatttctgc ccagccagtt ggtctcgccc
      121 cctcaagggc acgaagaatt caaaaatatt tattgtgtgt acaccggcgg ggatgatctg
      181 cttctggtcg gcccttggtc caccgtactg gaatttgcct tgtttctcaa caatgcattc
      241 cgccgctaca cctgcaacaa tccaaaagtt acgctttcag cggccgtggc ggtgattcat
      301 ccgaacatgc cgctggctcg ggccgcgatg caggccgatc agatgctgga agaggccaag
      361 gatgccggca gaaaccgcat ggccgtcttt aatgaagtga tgacatggga tgaatatgcc
      421 aaggcactgg acgacggcct ctttctgcac aaggcactca ccgacaaagg caccgacggc
      481 atcaagatca atcgcggatt tgtctaccgg cttctgaatt attataaaat ggcctccgat
      541 gaaaccaagg taaaaaacct gatctggcgt tcacacttgg cctacgacat cgctcgtaat
      601 gtcgaacagc ccggtgttcg ggattcacaa atcgatacac cgggccgccg gcgcatcaaa
      661 gaaatgaccc gctttggcat cgggcgcaag gacgaaatta aacggttaaa agtatccgct
      721 acctattgtt tatatctaaa caggaaagga gacaaaaatg gcgcatgcat ttgacaacag
      781 acaacgcccc tggcagcatg gaggaggcgg cactccccgg caggccgcct cagtcccggt
      841 aacgttctat cgcgatccgg ccaacaagaa acagctcaat ccgacactgc tgaatgaaga
      901 tgcggaaaaa caggcggcgg ccctgcacga taaaattaac tcggcccaga tacgcaggtt
      961 cttcagcgag gtcaaaaacc tgtccctgcg gctggagcag ggacgtccgt ggacgcagat
     1021 cgaaccgttc ttccggatgc ttcgaagtaa agcggagtat gcaagaggaa cgggaaaaat
     1081 tccgcctgat ttcgctaatt ttatttcaga caataccaaa cctgacaggg ttaaggatga
     1141 gcaggacttc agggcatttg tgttgtactt tgaggcggta ttgggttttg cttatggaaa
     1201 gggactggtg aaaaaatgaa ctggaaactg ataaacacaa aacggattac cggcaagatt
     1261 acagtcatca cgggcctgca tatcggcggt tcgacggaac tgatggaaat aggcggtgtg
     1321 gacaacccca ttatcaaaaa tccggttgac aattcaccct atattcccgg ctcctctctc
     1381 aaaggcaaga tgcggtcgct gatggaatgg aaactgggac tgctcagtgc caacggaaaa
     1441 ccctacacca gcgacaggcc cgattgtccc atcacacgcg tcttcggcgg catggcgggt
     1501 gccaccaagt tcggtccaac ccggctggtc gttcgcgatg cctttttaac cgctgaatcc
     1561 cgcaaggcat tcgatgaagg tcaggaactg accgaagtca agcacgaaaa ctccattgac
     1621 cgcatcacct ccgaggccaa cccccgtccg ctggaacggg tggttccggg agtcgccttt
     1681 gatctggata ttgcctacaa ggtgattgat accgatgatg gcggagcagt ggacgagaat
     1741 tacttccaag aggtcgtgct gagggctctt gccttggtgc aggaggactt tttgggcggc
     1801 tcgggcagcc gcggctgcgg gcgcatccgc tttgatgacc taaaagacga aaagggaaac
     1861 ccgcttcagc tgcccagtgc ctaaggaggg ctccaatgaa aacctacaaa ctcaaactgc
     1921 aggtctattc cgccttgcag acccccctgc agccggatac gctgtttggc cacctgtgct
     1981 ggtccatccg ctacacggaa ggtcagcagg ccctgcaggc gtttctggac agtttcatgc
     2041 aaggtccgcc gcctgtggtt ttgggcagcg cgtttcccga gggcttctgg ccggcgccgc
     2101 tgctgccggg tccgtcccgg cagcaggaag acatcctgct cgatatgttg tccaaaatat
     2161 cccgcacgga gctcaaaaac cggctgccgt tctgtccgct gagtgaaaat agcgtttcga
     2221 aaacactgac agatttggag gccttcgaca tcctcaaatg gctgcggaaa ctgcggtttc
     2281 ttcccgagga aatgctgccg gagtttgccg ccgcaatgga cagctttgcg attttgcagc
     2341 ggttccttca gaacggctgc ggcaaagcca atctcctgac ttccgctgcg gtcgcacaca
     2401 actccgtcaa ccgcctcacc ggcgcctcgg atgagctgta cttttccaat gaatattttc
     2461 ccaatcccaa aaaaccgccg gtctttcagc tggttgtgat gtccgatatc tgggatgccc
     2521 atcagattac ggaaaaattt acggcggccc tgcagggcgg ttacggcaaa agcaaaagcc
     2581 gcggcaaggg ctggattaag gtcatcggca cagagccgtt caccctgccg catgcccaat
     2641 cgcccaatgc cctcttgctt ctgggaacct gctgtccggc ccaaacggat ccggcacagg
     2701 gtttttggca atgtgcaaca aaagccggca aactcggcgg cgactgggcg gttggcgaca
     2761 atcccttcaa aaaaccgctg acgatgctgc tgcccggctc gctgctgaaa acggactctc
     2821 caaagtcgta ctgcgggcgg ctggtcaaag gcgtcagcga tgcctatccg caggtcgtcc
     2881 agtacgggct ggctccggtg ctggcagtcc gatgggatgc aaaggaggtt ctatgaaacg
     2941 ttataagatg acgattacta ttctttcgcc ggtgcatatc ggttccggcc agcagatcga
     3001 tccgctggag tatttggtca aggataccat cttttaccgg ctggataccg cacgcttttt
     3061 gagcaccctg acggccaatc tgaaagcaaa attcaatgag gccctcaaaa gcagcaatcc
     3121 cgtcactatg cgggaattta tagtcgataa tctggataca aaaaaataca gcctcttctc
     3181 cgccgcaaca ggaccggact tcgagaaggc ctatgcgaac aatgtcgaca gcatcaataa
     3241 ccagcttctg gtcgaattgt tcccccgctg cggcacccag ctgagggctt acctgcccgg
     3301 ttcatccatc aaaggagcaa tccgcacggc ggtggtttcc caactggcca aacccggcat
     3361 ttcgccggcc gtctatacgg acgaccgcgg acagcatccc tacaagagcg gtacgcggct
     3421 ggaaaaatac attctccgct gcaaagacgc caagcaggac cccttcaaga tgcttcgcat
     3481 cgccgacgcc ttcctgcctg acgatgcgac ctttattgat ctggctcaga tatacaagcg
     3541 cgaccgcgct gccggcgccg cggacccggg caaaatccag atattctgtg agcagtgttt
     3601 ttccatgctg gacaacgaac ggataaccgc cgaagggatg ctggagatca acactgatct
     3661 gatcggcaaa aaaggcgttg accgccgggg caattctgtc gatgcggtca gcatgcccct
     3721 gacggctgaa cagattctca actcctgcaa ggccttttac ctgccgaaga tggaccacga
     3781 acacaaggtc ttttacgacg acaacccgca gctgcggacc cacagccaaa aactgctcga
     3841 tgtaaaatat gccgacaacg aatgtcccat tcgcatcggc cggttttgcc atgtcgagtg
     3901 ccacacgctg gataactacc gggaaccgaa aacccgacgc ggctggggca agacccgcac
     3961 attgtcggcc ggcacgatgc cgatgggctg ggtcaaaatg cggctggatg agatgaaata
     4021 ggttttttta agaagcggtg ttctttgaca agttcatagc gttttcgggg gcaggcgaaa
     4081 gttatcttat cgcgactgtc tgccgttatg aatacggggt aacgctgtct atgcaaacgc
     4141 tgtatgtcaa agagcaaggc gccaaggtcg gctgcacggg acggcggttg acggtaacca
     4201 aagaagaaca ggaacttctg accgtcccca tctgccgcct gacgcgggtg gttctcatcg
     4261 gccgcgtgca gattacaacg caggcggcgg cgcttttatt ggaccgccgc atcccgctgg
     4321 tgatggccac gctgaccggc cggctccgcg gcgtgctggt gccgccggat acgccgcatg
     4381 taacgctgcg cctgaaccag tatgaactgg cgaaaaatcc gcaatacggc ttcaacttct
     4441 gccgcgattt ggtcgccgca cgggccgcca gcgcctgcaa tgttatcaga cgttatctct
     4501 acaatcaccc caaaaaagac ctgcgcccgc atatccagaa aatactggat tatgcctcgc
     4561 aagtcagcaa tcagccctcg atcgattcgc tgcggggcat ggaaggaatg attgcccggg
     4621 aatattttgc agcgctggta aaaatctttc aggatttggg aatgaatttc agcggacgca
     4681 tccgacggcc gccgaccgat ccggtcaacg cctgtctgtc ctatacctac atcctgctga
     4741 ccgaacactg ctcctgcgcc ctgcagacaa cccggctgga tatctatctg ggctttatgc
     4801 atcagcccaa ccgcaatgcg ccggcactgg cgctggattt tgttgagcag ttccgccagc
     4861 ccgtcgctga ccggttcgtg atgctgctat ttaataaaaa aatgctgcag ccgtctgact
     4921 tttccccggg cccgatgaac gctgtcctgc tgtccgaaca ggccaagcgc acgctgatga
     4981 cccaatggga gatctttctg gataccccgc agcggctgct tgaaaatcag cccgccttgt
     5041 cgccccgcga cctgctctat cggcaggctg aggcgatgga agcggccgtc cggcagaaac
     5101 agcccttctg ccattaccga ttgtgcctgt gaggagacag catgtttctg gtgatcggct
     5161 acgactgtac agatgacaag cggcggctca aaatcgccaa aatcctgctt gattacggct
     5221 atcgtgtcca gtacagcgtc ttcgaagccg atctggatca gccccttctg gatgagctga
     5281 ttaagcggct tgccaaggtc attcatccgg gcgaggattc tattcgcatc taccatatct
     5341 gccagcggtg tctggagcag acacaacttt ttggggatgc ccaattgaca catcaggaaa
     5401 aagtgtttat agtataaaaa aaagaaaccg gcagacaggt tatggaaaaa ttgcaagatg
     5461 tttgctgata agaacttaca gaggttttgg agccgccgtc aacggcttga tacgctgcca
     5521 tgcagcccgg cccaaggcgg ctatgaaata cgcatttgca acttccggca gataaagaac
     5581 ttgcaaacgc cgcagttgga attaccccct gatgaataag ggattgaaac ttttctcctt
     5641 tccgccggct tttgccggct gctgctgttg gaattacccc ctgatgaata agggattgaa
     5701 acacttgata gacccgccgg agaagggcaa aaccgaatat ctgttggaat taccccctga
     5761 tgaataaggg attgaaactc tttcagctca accatcctgg taaagtgatt c
//
LOCUS       DAOTBO010000139         5742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_11744_length_5742_cov_8.486900, whole genome shotgun sequence.
ACCESSION   DAOTBO010000139 DAOTBO010000000
VERSION     DAOTBO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11744_length_5742_cov_8.486900"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..364)
                     /locus_tag="PLE88_11805"
     CDS             complement(77..364)
                     /locus_tag="PLE88_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77190.1"
                     /translation="MIADCYVKLKAEGVVDPNEADPVIEWGYKTLFDQYPDSRDVEYA
                     AIRLIEINLARGKPVTAAVYINWLLDHTASGDPRRMNVIHKLMAGLEVCGQ"
     gene            complement(631..1461)
                     /locus_tag="PLE88_11810"
     CDS             complement(631..1461)
                     /locus_tag="PLE88_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77191.1"
                     /translation="MKMMACLKYLSISVIAIILTSPSAYASKEATKNNSYEKKFTLLM
                     RHIHEMDMTEDKEKVFAQIRIEGKIRWLLDDTVKNIVIELTEIKRSFSHALAEKTSPK
                     FEPFTTEIVINPQQESPVAIMLVTGRSDSEIVRQSQYMVIYMILDMLLESGEHIIEQP
                     KGKGMGLLKIMTYESFDIDGIKGVKPESLRSYSQKTSFNIIPDIKEAKLKGLHLVSDA
                     GNNYYYRYTDNTTGIVDHAINFEAVPADSAPFDYLKILGKTQDVNLFQYLDYSELMLQ
                     "
     gene            complement(1424..3229)
                     /locus_tag="PLE88_11815"
     CDS             complement(1424..3229)
                     /locus_tag="PLE88_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77192.1"
                     /translation="MKTKTIFYGAFLVSIVLVSFPVFAWPPPPCPGCQTRWPSDGQCH
                     DDDSKCPRFCVNASCVECKETEHCGGCQYCNSSWACAPVSVATLYADEEVVCVGYTDW
                     FSATTSPGGYTCQLVWQHEGAAESGSICGSSWARWTSPGTYTVTASCGSSSQSKTITV
                     VGVTRVDAEPNAVCVNDLNGILFTAVPYPSGSMNCIVWERRFKANSSDAWSDWYGCGA
                     HETTNRLPTDEPGFYQVRAKNGINDEGAESDVVKVVKVDLVAQPHVQPFSPLVAGRIC
                     ANAQQLFREAPWKAIVKPGGTTATVAVSGPVSGGGTVSDNSIFYVESIDEDQVGVYTV
                     TITHDDCGDCPVQKNETVFKFIFDTYRYPDGDSGASSPPCSQHSTASGYANAKADESA
                     LEGHEYGAKVIWCYRVKVLDDPLGSYEGSVKARAIVNYQTTGSMILRNGPPANWLTVK
                     APSINLTFDIISASVAANLGLVDYGSSLAGANISSVIADGEEFKCATVSHNWNEVGLP
                     FPIWGVHYKYKMWDDTGLLELHEDINRTYSVGSPVSLNVKASASASISQLIYFGPTEE
                     IPEQILRSPVVLDNTQHISTVEVYEDDGVFEIPVN"
     gene            complement(3226..5346)
                     /locus_tag="PLE88_11820"
     CDS             complement(3226..5346)
                     /locus_tag="PLE88_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77193.1"
                     /translation="MRISGTSFHRAIFTVFAGICLCFYCSLVFAQEEVSPFDAQVRAI
                     NEVIQQKNLSQAQTSVDQFWAEHSGEEGFVDAVRRVKDAYWTNEFYPAHFDLCERIVT
                     AFPNDPLSIGIQADRVTGYIARKDLSRAAEELDRFWDLYSRDERFVDFARRIKDHYWW
                     GKHYAEHFQFCDELVSLLPNDPLTVGILADEVNGYVILKDSEKAAQKLDELWVRYSTH
                     PDFVEKLKNIKEFCWLNENYPLHFSICERIAQEFPDHPLTIGLLADEVGGYINLNDPN
                     SAAETLDRFWTRYHTHPDFLEQFQKIKNVCWWSKNYAMYFPLCQRFVEQFPNDPAAVG
                     LLADQVNGYVMLKESDKAAETFERFWNQYSTHPDFLEQIKNTKNICWWNKNYALHFAI
                     CQRVAQAFPDDPRTIQVRADEVTGYLDRGDMDKAETLVQQFLSEPHTGDEVVTFLNPV
                     ANLYNLKGDYPKADALYQHVLDSADADDGRLVIAKMGQLASRIATTDPNIIQQQFDRL
                     LTDYADDPNLVKGVLPVAEVYYLKGQTDFSRGNTAAANESFDQAIALLDSILPVVSDA
                     HDQSLACYMKGLSYWYRGDLFKSSEALLDSLDLFPNHPHAGSTCRMIADCYVKLKAEG
                     VVDPNEADPVIEWGYKTLFDQYPDSRDVEYAAIRLIEINLARGKPVTAAVYINWLLDH
                     TASGDPRRMNVIHKLMAGLEVCGQ"
BASE COUNT         1409 a         1551 c         1330 g         1452 t
ORIGIN      
        1 ggccaggcga gggccgtccc actcaaaagg atatagattc caagtaaaac aaggcgaaat
       61 aaggtgtttt tcgttttcat tggccgcaca cctccagtcc agccattaac ttgtgaatga
      121 cattcatccg ccgcgggtca ccagaggccg tatggtccaa aagccaattg atatagacgg
      181 ccgccgtaac cggtttgccg cgcgccaggt tgatctcgat cagccggatc gccgcgtact
      241 ccacgtcccg gctgtccgga tactggtcaa acagggtctt gtatccccac tcgatcacgg
      301 ggtccgcttc gttggggtcc accacaccct cggccttgag tttgacatag caatccgcga
      361 tcatccggca ggtcgaaccg gcatggggat ggttggggaa caggtcaagc gaatccagaa
      421 gcgcctccga cgcgttattt acttgccaaa gagcagcaac cccgccttaa actaaatcct
      481 acaacacccg cgtgggcaaa tcaacgattt gcgccaccct gcgaaaaatt gcccgccctg
      541 agcgaagccg aatggcccgt gctacccggc tgaaagagag aaattagtat ggtgtcacca
      601 gattttgatt tagatttgat ttaaacttct ttattgaagc atcaactcgc tataatcaag
      661 atactgaaac aggttgacat cttgcgtttt gcctaatatt ttaagataat caaatggcgc
      721 tgaatccgca ggaaccgctt caaaattgat cgcatgatca acgattccgg tcgtgttatc
      781 tgtataccgg taatagtaat tattgccggc atcagaaacg agatgaagcc ccttcaattt
      841 agcttccttg atatccggaa taatattaaa actggttttt tgtgaataac tacgcaagct
      901 ctccggtttt acgcctttta taccgtcaat atcaaacgat tcatatgtca ttatctttaa
      961 aaggcccatt cctttacctt ttggctgctc gattatatgt tcgccggact caagtaacat
     1021 gtccaaaatc atataaatca ccatatattg actctgccta acaatttccg aatcgctgcg
     1081 gccagtcacc agcataatag caaccggtga ctcttgctga ggatttatta caatttcagt
     1141 cgtaaaaggt tcgaacttcg gtgaagtctt ttcagccagt gcgtggctaa atgaacgctt
     1201 gatttctgta agttcgatca cgatgttttt tacggtatcg tctaaaagcc atcgaatttt
     1261 tccttcaatc cgtatttggg caaaaacttt ttctttatcc tctgtcatat ccatttcatg
     1321 aatatgccgc attaacagcg taaatttctt ttcgtaagaa ttgttttttg ttgcctcctt
     1381 tgaagcgtat gcagagggtg atgtcaggat aatcgcaata acgctaattg acaggtattt
     1441 caaacacgcc atcatcttca taaacctcca cagttgaaat atgttgagta ttatctaata
     1501 caactggact tctaagaatc tgttccggaa tttcctctgt tggtccaaaa taaatcaatt
     1561 gtgaaatgct ggcactcgca gaagctttaa cattcagcga tacgggagac cctactgagt
     1621 atgttctgtt aatgtcttca tgcaattcca gtaaaccggt atcgtcccac attttatatt
     1681 tatagtgtac cccccaaatt ggaaatggaa gaccaacctc attccaatta tgagaaaccg
     1741 ttgcacactt aaattcttcg ccatctgcaa taactgatga aatatttgcc cctgccaggg
     1801 atgaaccata atcaactaaa cccaaattag ccgcaacaga agcagagata atgtcaaaag
     1861 ttagattgat tgagggggcc tttacagtca gccaatttgc tggcggtcca ttccttaaaa
     1921 tcatagaacc tgtggtttga taattcacta ttgctcgtgc ttttacagaa ccttcatagc
     1981 ttcccaaagg atcatctaac actttaacac gataacacca gattactttt gcaccgtatt
     2041 catgaccttc caaagccgat tcatctgctt tggcattggc gtagccgctg gctgtgctat
     2101 gctgcgaaca ggggggtgag cttgctcccg aatccccgtc aggatatcga tatgtgtcaa
     2161 aaataaattt aaacaccgtt tcattctttt ggacggggca gtcgccgcag tcgtcgtggg
     2221 tgatggtaac agtatatacg cccacttggt cctcatcaat gctttcaaca taaaaaatgg
     2281 agttgtcgct gacggttccg ccgccgctga cgggcccgga aacggcgacc gtcgccgtcg
     2341 tgccgccggg cttgacgatg gccttccacg gggcttcgcg gaataattgc tgcgcattgg
     2401 cacaaatccg ccccgcaacc agcggcgaaa acggctgcac gtgcggctga gccaccaggt
     2461 ccacctttac gaccttgacg acgtccgact ccgccccttc atcgtttatc ccgtttttgg
     2521 cgcgtacctg ataaaatccc ggttcatcgg ttggcaaccg atttgtcgtc tcatgggctc
     2581 cgcaaccata ccaatcagac caggcgtcac tggaattggc tttaaaccgg cgctcccata
     2641 caatgcaatt catcgaccca ctggggtacg gaactgccgt gaataaaatt ccatttagat
     2701 cgttcacaca gacggcattc ggttctgcat ctactctggt tacccctaca acggtaatgg
     2761 tttttgattg actgctgctg ccgcaactgg cggttactgt ataggtcccg gggctggtcc
     2821 agcgcgccca ggaagagccg caaatagagc cggactcggc ggcgccctca tgctgccaga
     2881 caagctggca cgtataaccc cccggactgg ttgtggcgct gaaccagtcg gtgtagccga
     2941 cgcagacgac ctcctcgtca gcataaagag tcgctacgga aaccggcgcg caggcccagg
     3001 atgagttgca gtattggcag ccgccgcaat gttcggtttc cttgcactcc acacaacttg
     3061 cattgacgca gaaacgcgga cacttgctgt catcgtcgtg acattggccg tcgctgggcc
     3121 atcttgtctg acaacccggg cagggcggcg ggggccaggc aaacaccgga aacgatacca
     3181 agacaataga aaccaaaaac gctccataaa atatagtttt cgttttcatt ggccgcacac
     3241 ctccagtcca gccattaact tgtgaatgac attcatccgc cgcgggtcac cagaggccgt
     3301 atggtccaaa agccaattga tatagacggc cgccgtaacc ggtttgccgc gcgccaggtt
     3361 gatctcgatc agccggatcg ccgcgtactc cacgtcccgg ctgtccggat actggtcaaa
     3421 cagggtcttg tatccccact cgatcacggg gtccgcttcg ttggggtcca ccacaccctc
     3481 ggccttgagt ttgacatagc aatccgcgat catccggcag gtcgaaccgg catggggatg
     3541 gttggggaac aggtcaagcg aatccagaag cgcctccgag gatttaaaaa ggtcgccccg
     3601 ataccagtag ctcaaccctt tcatatagca cgccaaactc tggtcatggg catccgacac
     3661 gaccggaaga atcgaatcca gaagggctat cgcctggtcg aatgattcgt ttgccgcggc
     3721 ggtattgccc cggctaaagt ccgtttgccc tttaagataa tacacttccg ccaccggcaa
     3781 aacgcccttg accagattcg ggtcgtccgc atagtcggtc agcaaccggt cgaactgctg
     3841 ctgtatgata ttcggatcgg ttgtcgcgat tcgcgacgcc aattgtccca ttttcgcgat
     3901 caccagtcgc ccgtcgtctg catcagcaga atccaggaca tgctggtaca atgcatcggc
     3961 tttggggtaa tcccctttca ggttatacag attcgccacg gggttcagaa acgtcacaac
     4021 ttcgtcgccc gtatgcggtt cggacaaaaa ctgctgcacc agcgtttcgg ctttgtccat
     4081 atcgccgcgg tcaagatacc ctgtgacttc atccgcgcgt acctgaatcg tccgcggatc
     4141 atccgggaag gcctgggcca cccgctggca gatggcaaaa tgaagggcat agtttttgtt
     4201 ccaccagcag atattcttgg tattctttat ctgttccaga aaatcaggat gcgtactgta
     4261 ttgattccag aaccgctcaa acgtctcagc ggccttgtca gattccttca gcatgacata
     4321 cccgttgacc tggtcggcca gcagacccac agccgccgga tcgttcggga actgctcgac
     4381 aaaacgctga cagagcggga aatacatggc gtagtttttg ctccaccagc agacgttttt
     4441 gatcttttga aactgttcga gaaaatccgg atgggtatgg tagcgggtcc agaaccggtc
     4501 cagtgtttcg gcggcactgt tcggatcgtt gagattgata tagccgccca cctcgtcggc
     4561 cagcagaccg atagtcaacg ggtgatccgg aaattcctgc gcaatgcgct cacagatgga
     4621 aaaatgcagc gggtagtttt cgttcagcca gcagaactcc ttgatatttt ttaacttctc
     4681 gacaaaatcc ggatgtgtgc tgtaccggac ccagagttca tccagctttt gggcggcttt
     4741 ttccgaatcc ttgaggatga catacccatt gacctcgtcg gccagtatcc cgacagtcaa
     4801 cgggtcgttc ggcagcagcg aaaccaactc atcacagaac tgaaagtgtt ccgcataatg
     4861 cttaccccac caataatggt ccttaatccg gcgggcaaaa tcgacgaatc gttcatcacg
     4921 gctgtacagg tcccagaacc ggtccagctc ctcggcggcc cttgataaat ccttacgcgc
     4981 gatatagccg gttacccgat ccgcctgaat cccgatggac agcggatcgt ttggaaaggc
     5041 cgtaacgatc ctctcgcaaa gatcaaaatg tgccggatag aattcgttgg tccaataggc
     5101 atccttaacc cgcctaacgg catcaacaaa tccttcctcg ccgctgtgtt cggcccagaa
     5161 ttgatccacg gaggtttgcg cttgagaaag atttttttgt tgaatgactt cattaatcgc
     5221 ccggacttga gcgtcaaagg gggacacttc ttcctgtgca aaaacgaggg aacagtagaa
     5281 acatagacag ataccggcaa atacggtaaa aattgcccga tgaaatgacg tgcccgaaat
     5341 tcgcatacgc tttctccttt cggtgcagaa cagacttgtt ctgcacagct tatgcgagat
     5401 ttagaagaag atgcccgccc ttgccattcc gaacccatcc ctgcgcttgc ccccggaaca
     5461 agtccggggt gacattgctt cgggctgttg tctcgggtcg cttacgctgc ccgcatggaa
     5521 tgaccttctg ggttctaaat tctccctgtc cgttattcct ttttcacgcc ctgtacgctt
     5581 ctctgatgcc gtctgttgaa ccctgcgtac aataaaggac tcccatcatt acatccaccg
     5641 tagtaccccc ccccacgact ttgtcaagca caaaattgcc agaattttaa tttttttttc
     5701 atccatctgt aaagggcgtt tttttcttta ctatactatt gg
//
LOCUS       DAOTBO010000140         5634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12060_length_5634_cov_4.005019, whole genome shotgun sequence.
ACCESSION   DAOTBO010000140 DAOTBO010000000
VERSION     DAOTBO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12060_length_5634_cov_4.005019"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1874)
                     /locus_tag="PLE88_11825"
     CDS             complement(30..1874)
                     /locus_tag="PLE88_11825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOM77194.1"
                     /translation="MRLQAIEPQIRFEPLPPSEPPLPPEADKQWQSVPAGQRQELFPV
                     LDYRYTWSSAPLLADHTDAAWTAPVWRKSLSEISWELDKTSDNKFGSLQAAEATPVTG
                     AWQSLRSVFSDTLITPDGSIITNPTRAERLDVAIGAISDPAIYLYARYFTGRMFQLPR
                     LVYAGLNRLIPEAFNEPKDFSDAVLDALEYQPGKFVKSIGYLSDFVGSLRAASPASLP
                     KSLNLLHKMGDDVLRNAAAIEMQELAKRAAQAVSPNAPQETMTAGQAADMLLWTSGFS
                     ALFSSAAAFFKSPLGTQMQFYLLSKIGKYLVDHPNVSKWVLPSQMSRQQEELYAGLHK
                     ILQQQGIDTSNLSYEQRAALAVSAKLGTRLMRLHRQLFQDEDIIKASKPASGVSAQTA
                     LSAEPVFTASHSAAMQALGYTDDVIWSTPTSEAKRIVESGILAESSKKIQQAAKSAEI
                     APKETADWQINSITREDGGLLLSASREGKEAGYAIVTNLGDGIFELNEIRVNDTFKRQ
                     GLGTELYRNAAQKVKALGGKLYTSPEMTDAGKALRDSIDKAGGLGNNTRVKGAYRREV
                     LSSFNPPAATSCWIGSGTTKGDSLRIPFLAHDVGITAAKGSTVMTRTF"
     gene            complement(1871..2005)
                     /locus_tag="PLE88_11830"
     CDS             complement(1871..2005)
                     /locus_tag="PLE88_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77195.1"
                     /translation="MIRKTSNGYVVYSEGGRRLSRPLTSRRAAERRLRQIEYFKHQHK
                     "
     gene            complement(2002..3624)
                     /locus_tag="PLE88_11835"
     CDS             complement(2002..3624)
                     /locus_tag="PLE88_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77196.1"
                     /translation="MGQGLQDYAELRQLLHDKYQSHLDDLRTAQAKDLLNIAAIRIDG
                     IYENQADPARWEESVRLELDDAAQRIGELLEDVSQQRRPFIDTYVKAFHGAEIAKMES
                     LRAKTAAKLNLEAMSASYQQAYANGQTQAGEAIYNEMVQTNRYLDWFPNRDAFDIHMA
                     RHREAGQTEHYQGIIRQAKTDVAQAVQYSPDDAEDIAKTYAEQYQLDAADALDLRQYA
                     KSAANAFKTKQADAYAAIEANVSRQLRDRYIDGSLTEDFIDEVWQKTRVADDKQSQFD
                     DFIKEWRILFRQKMEQSTAKPKTNTAVFNALHNKAIEVGLGAGDPNRFALTVLRAENK
                     GFLTSEDAKGLLRLAHTQQQSHISTVRSSLESEVRSQMVDASVATPDAFSATLSVMMA
                     NPGLSDEEIQRRTEELQIKRQVQEWNYSRYKRSMDAFLESNPKADQNAIRGYSDYLLR
                     MYAKPYTDVVKDYREAMGGLPVFPPVAPSGQRADAVREWAKQSFGKEPRYDEKAGAFV
                     VDIDTGGTVRTYKVVIDNPENADDVLLKEFDL"
     gene            complement(3575..>3799)
                     /locus_tag="PLE88_11840"
     CDS             complement(3575..>3799)
                     /locus_tag="PLE88_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77197.1"
                     /translation="AVYELCRIGDDDIDHRKLKGGLNVKIPAVQLIRPSPPSSACGGT
                     TGLILGCACTGFKPDGARPAGLCRIAAASA"
     assembly_gap    3800..3809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3810..4306)
                     /locus_tag="PLE88_11845"
     CDS             complement(<3810..4306)
                     /locus_tag="PLE88_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77198.1"
                     /translation="MSSGALIVAALAASGTASVAQGLAAHAQGRANQRIAEYNAAMAE
                     RQGKAQMEAARLNSERLARQQRLAMGQNIAAAAKSGISLSGSVLDVLADTAYQYQLDR
                     NLILTQGLNDYQTGSQRASLLRSEGKFSAAQGRSNALWAYLNGVSSVALGLARYDLLK
                     EDLAR"
     gene            complement(4288..4713)
                     /locus_tag="PLE88_11850"
     CDS             complement(4288..4713)
                     /locus_tag="PLE88_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77199.1"
                     /translation="MHLRFYQSGDYSQITDPVEPAVPNIPFSEASLAVDIHPVALTLT
                     SGGIPIACGGVIVSEDGTGGEVWLRVSAQCAQRYPIVLLRTLKAGKAILADEFGFRWL
                     SARIQWNFAAGRRLAERFGFRKVRDDNEAFEVYQCHLEH"
     gene            complement(4724..>5634)
                     /locus_tag="PLE88_11855"
     CDS             complement(4724..>5634)
                     /locus_tag="PLE88_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77200.1"
                     /translation="ACYWGAVDDLRIYKKALSASEVAAIYNHGGGRPYSGSILEGGEA
                     SAVFEFNEGSGTSTVDSVSGITGTVYYCVWELGGIPFHSGTELIVYLPNHGYSNGDFV
                     YVSWLNRVCCVGNVTQNTFMLYESLVGNPIYFVNELTSGYVQLADTSQSHTIISGLEH
                     LEGNTVHVASNGRLVGSFAVSGGQIAIPIDATNYIVGLPYAMKARTMRLELPQAQALQ
                     SRTKRIHKASVRYVRSVDGAAGQEYDNKEYLSDLHAAYSTQSDDAEVLVKGGFAEDGF
                     LVVRSDTPTPFTVLSASIGFSVDEPA"
BASE COUNT         1230 a         1583 c         1402 g         1409 t
ORIGIN      
        1 acaaattttg tcataatttt cccctttcat cagaatgttc tcgtcattac agtacttcct
       61 ttcgcagcag taattccgac atcgtgggca agaaatggta tccgaagcga atcgcctttt
      121 gttgtcccgc ttccaatcca gcaggaagtt gcggcagggg gattaaaaga ggaaagcact
      181 tctctgcggt atgcaccttt gacccttgta ttatttccaa gtcctccggc tttatcaatg
      241 ctatccctta atgccttgcc tgcgtctgtc atttcggggg atgtgtatag tttgcctcca
      301 agtgctttta ctttctgtgc ggcattacga tacaattctg tgccaagccc ttgcctcttg
      361 aaagtatcat taaccctgat ttcgtttaac tcgaaaatac catcgcccaa atttgtcact
      421 attgcataac ccgcctcttt gccttcgcga gaagcagaaa gtaacagtcc tccgtcttct
      481 cttgttatgg aatttatctg ccaatcggct gtttctttgg gggctatttc ggctgacttc
      541 gcagcctgct ggattttttt cgacgactcc gccaagatgc cgctttcaac aatccgcttg
      601 gcctcactgg tgggtgttga ccatatcaca tcgtcggtgt accccagcgc ttgcattgca
      661 gcgctgtggc tggcggtaaa aacgggctcg gcagataatg ctgtttgtgc ggatacgccg
      721 gatgccggct tggacgcctt gataatgtct tcgtcctgaa ataactggcg atgcagacgc
      781 atcaagcgtg tacccagttt ggctgatact gccaatgcag cccgctgttc atacgataag
      841 ttggatgtat caattccctg ctgctgaagg attttgtgta gtcccgcata cagctcttcc
      901 tgctgtctgg acatctgcga cggcagcacc catttggata cattggggtg gtcaacgaga
      961 tatttgccga ttttgctcag caggtagaac tgcatttgcg tccccagcgg cgacttaaaa
     1021 aacgccgccg ccgagctgaa taaagcagaa aacccagatg tccacaacag catatctgcc
     1081 gcctggccgg ctgtcatcgt ctcctgcggc gcattggggc tgacagcttg tgcggcacgc
     1141 ttggccaatt cctgcatctc gatggcagca gcattgcgca ggacatcatc gcccattttg
     1201 tgcagtagat tcagcgattt cggcagggat gccggcgaag ccgcccgcag gctgccgaca
     1261 aagtcggaca aatacccgat ggatttaaca aacttgccag gctgatactc caaagcatcc
     1321 agcacagcgt ctgaaaaatc cttgggctca ttgaatgctt ccggaatcag cctgttcaag
     1381 cccgcataaa ccaatcgtgg cagctgaaac atcctgccgg tgaagtacct ggcgtacaaa
     1441 taaatcgccg gatcgctgat tgctccgatt gccacatcca atcgctcggc acgggtggga
     1501 ttagtaatga tactgccgtc tggggtgatt agagtatccg aaaagacaga ccgcaggctt
     1561 tgccatgctc ctgttacagg tgtagcctcc gctgcctgca gactgccgaa tttattgtct
     1621 gatgttttat ctaattccca tgaaatctct gaaagtgatt tcctccaaac cggagccgtc
     1681 caggccgcat cggtgtggtc ggcaagcagt ggggctgaag accaagtgta ccgatagtcc
     1741 agcacaggga acagctcctg acgctgccca gccggcacag actgccattg cttgtcggcc
     1801 tccggcggca atggaggctc gctgggcggc aggggctcaa accgaatttg gggttctatg
     1861 gcttgcagtc tcatttgtgt tggtgcttaa agtattctat ctgccgcagg cggcgttcag
     1921 cagcccgccg gctggttaat ggacggctta acctgcgtcc tccttcacta tacaccacat
     1981 atccattgct ggtcttgcgt atcataaatc gaattccttc aaaaggacat catcggcatt
     2041 ttcgggattg tcgattacaa ccttgtatgt ccgcaccgtt cctccggtat caatgtcgac
     2101 cacaaacgca ccggcttttt catcgtatct cggctctttg ccgaaactct gcttggccca
     2161 ctcccgcaca gcatcggccc gctggccgga aggggcgaca ggcggaaaca caggcagtcc
     2221 gcccatcgct tcacgataat ctttgacaac gtccgtgtac ggcttggcgt acatccgcag
     2281 caggtaatcg gagtaccccc gaatcgcatt ttggtcggct ttggggttag attccaaaaa
     2341 agcatccatc gaccgcttgt accggctgta attccactcc tgcacctgcc gcttaatctg
     2401 aagctcttca gtccgcctct ggatttcctc atcagacagc ccagggttgg ccatcatcac
     2461 ggacaatgta gcgctgaagg catccggtgt agcaacagac gcatccacca tttgcgacct
     2521 cacctcgctt tcaagggagc tgcggacagt gctgatgtgc gattgctgct gggtatgggc
     2581 cagacgcaga aggcccttgg cgtcttcgga tgtcaaaaag cctttgtttt ccgcacgtag
     2641 cactgtcaat gcaaaccgat tagggtcgcc agcgccgagg ccgacttcaa tcgccttatt
     2701 gtgcagagca ttgaacactg cggtattggt cttcggcttg gccgtgctct gctccatctt
     2761 ttgtcggaac aggatgcgcc attccttaat aaagtcgtca aactgcgact gtttatcatc
     2821 ggcgacccgt gttttctgcc agacctcgtc aataaaatct tccgttaagg atccgtcaat
     2881 gtatctgtcc ctcaactggc gggatacatt ggcttcgatt gcagcatacg catcggcctg
     2941 cttggtcttg aatgcattag cagcgctttt ggcatactgc cgcaagtcca gcgcatcggc
     3001 ggcgtccagt tggtactgct ctgcgtaggt tttggcgata tcctctgcgt cgtctgggct
     3061 gtactgtacg gcttgggcaa catcggtttt agcttgccga ataatgccct gataatgctc
     3121 cgtctgtccg gcttctcggt gcctggccat gtgaatatca aaggcgtccc tattgggaaa
     3181 ccagtccaga taccgattgg tctgcaccat ctcattataa atcgcttcgc cagcctgtgt
     3241 ctggccgttt gcatacgcct gctggtagct ggccgacatg gcttccagat tcagtttggc
     3301 ggctgtcttg gcccgcaggg attccatctt ggcgatttcg gctccgtgaa acgccttaac
     3361 ataagtgtcg ataaacggcc gcctctgctg tgaaacatcc tccaacaatt cgccgatccg
     3421 ctgggcagca tcatccagct ccagacggac actctcttcc catctggcgg ggtcggcttg
     3481 gttctcataa ataccgtcaa tgcggattgc agcgatattg agcaggtctt tggcttgtgc
     3541 tgtgcgaagg tcgtctaagt gggattggta cttgtcatgc agaagctgcc gcaattcggc
     3601 ataatcctgc aggccttgcc ccatccggct taaagcctgt gcaggcgcat ccgagaatga
     3661 gaccggttgt gcctccacag gctgaggaag gggggctggg gcggatgagc tgtaccgcag
     3721 gaattttgac atttaatcct cctttcagct tcctgtggtc gatgtcgtca tcgccgatgc
     3781 ggcagagttc atagactgcn nnnnnnnnnt atctggccaa atcttcttta aggaggtcgt
     3841 atctggccaa acccaaagcc acactcgaga caccattcaa gtaggcccac aaggcattgc
     3901 ttcggccttg tgccgcacta aacttgccct ccgaccggag cagtgatgcc cgctggcttc
     3961 ccgtttggta gtcattaaga ccctgcgtca ggattaagtt cctgtccagt tggtactgat
     4021 acgccgtatc ggccaacaca tccagtacac tgccggacag cgatataccg gattttgccg
     4081 ccgcagcgat gttttggccc atcgcaagac gctgctgccg agccaagcgt tcgctgttga
     4141 gacgggcggc ctccatctgt gctttaccct gccgctctgc cattgccgcg ttgtactcgg
     4201 caatccgctg gtttgctctg ccttgtgcat gcgcagccag accctgcgca acagacgcag
     4261 ttcccgatgc cgccaaggca gccactatta atgctccaga tgacattggt acacctcaaa
     4321 agcctcgttg tcgtctctca ccttcctaaa cccaaacctt tccgccagcc ggcggcctgc
     4381 cgcaaagttc cactggatgc gtgccgacag ccatcggaag ccaaactcat cagcaagaat
     4441 agccttgcca gctttcagtg tgcggagcag gacaatggga taccgttggg cacactgcgc
     4501 tgagactctc agccaaactt ctccgcccgt gccgtcctca ctgacaatca cgccgccgca
     4561 ggcgattgga atgccgccgc tggtcaaggt cagggcgaca gggtgaatat ccaccgccag
     4621 tgatgcctcc gagaaaggaa tattcggaac ggcaggctcg acggggtcag taatttgact
     4681 gtagtcgcca gattgataaa accttaaatg catagaattc tcgttatgcc ggctcgtcta
     4741 cactaaaacc aatggatgca cttaatactg taaatggcgt tggagtgtcg gaacgcacca
     4801 ccaaaaagcc gtcttccgca aaacctccct tgaccaaaac ttctgcatca tccgactgcg
     4861 tagaatatgc agcgtgcaga tccgataaat attctttatt gtcgtattcc tggccagccg
     4921 caccatcaac tgaccgaaca taccgcaccg acgccttgtg tattcttttt gtgcgggact
     4981 gcagggcctg cgcctgtgga agctcaagcc gcatggtgcg ggccttcata gcatacggca
     5041 ggccgactat ataattggta gcatcaatgg gtatcgctat ttgcccgccg gacactgcaa
     5101 acgaccccac aagcctgccg ttggacgcaa catggacagt attgccctct aaatgctcca
     5161 gcccgctgat aatggtgtgg ctttgtgatg tatccgcaag ctggacatat ccagatgtca
     5221 gctcgttgac gaaataaatc ggattgccga caagcgattc atatagcata aatgtgttct
     5281 gagtgacatt gccgacacag cagactcggt tcagccacga gacatataca aagtcgccat
     5341 tggaatagcc atgattgggt aagtaaacaa tcaattctgt accggaatgg aacggtatac
     5401 cgcccaattc ccacacgcag taatacacag tccctgtaat gccggataca ctgtcaacag
     5461 tgcttgttcc tgagccctca ttgaattcaa ataccgcaga tgcttctccg ccttcgagaa
     5521 tgctcccgct gtaaggccgc ccgccgccgt ggttgtaaat agctgcgacc tctgatgctg
     5581 acaaggcttt tttgtaaatt cgcaagtcgt ccactgcacc ccaataacag gcat
//
LOCUS       DAOTBO010000141         5629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12073_length_5629_cov_3.568712, whole genome shotgun sequence.
ACCESSION   DAOTBO010000141 DAOTBO010000000
VERSION     DAOTBO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12073_length_5629_cov_3.568712"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLE88_11860"
     CDS             <1..895
                     /locus_tag="PLE88_11860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM77201.1"
                     /translation="KVLQTKATVSEARLKLEKYEHVRRLSDNKVPSQSEFDAAQAAYD
                     RAVADEASAEAAEAQAQATLEAYQTDLSKAVIYSPINGIVLTRSVEPGQTVAASFTTP
                     VLFTLAEDLTKMELHVDVDEADIGQVKEGQNATFTVDAYPNQSFNAQITQVRYGSTTA
                     SGVVTYETVLEVANNDLSLRPGMTATADIIVIRAEDILTIPNSALRFKMAEAAEAQNT
                     NGSIVDSLLPHPPKHESKQQETAIVSGSTQQIYVLKEGKPAAVSITTGVTDGINTQVL
                     DGEIKEGMPVIIGTIAQEKKI"
     gene            892..1617
                     /locus_tag="PLE88_11865"
     CDS             892..1617
                     /locus_tag="PLE88_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOM77202.1"
                     /translation="MIQSAYSGNNQNVIDLQDIKKIYGRGQAVMQALRGISLKIERGD
                     FVAIMGPSGSGKSTCLNILGCLDAPTEGDYIFNGIRVNELDRNQRALLRRHYLGFVFQ
                     GYNLLNRTSALENVELPLIYRGIGVKERTKRARQSLEKVGLLEWQTHTPGELSGGQQQ
                     RVAIARAIVTEPEVLLADEPTGNLDTTRSHEIMDLLVNLNRDLGITIVMVTHEPDTAA
                     YAKRVIHFLDGLIASDKKNGELK"
     gene            1617..2825
                     /locus_tag="PLE88_11870"
     CDS             1617..2825
                     /locus_tag="PLE88_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOM77203.1"
                     /translation="MLLWDTLLIALREIRRHILRSFLTVLGIVIGVASVITMVTLGNG
                     ATVQVTQQIKSMGSNLLMVTAGKRLGPGRDSSAPSFKIADAEAIARDISSIDAVAPAS
                     SQSVTAVFGNENWSTTVTGTTNQYLRARNCAIQTGREFSTSELRAGAAVCIIGDTIRK
                     KLFGPQDPVGKRLRLGKLSFQVVGLLEAKGQSSMGQDQDDIVLIPIKSFQRRLSGNTD
                     VGIILVSASDNASTEAVKEDIENLMRERRHISKNEEDNFNVMDMTEISNMLTGTTRIL
                     TALLGAVAAVSLVVGGIGIMNIMLVSVTERTREIGIRLAIGALEREVLMQFLVEAVVL
                     SSAGGLLGIVVGLLMCAGLKGVLQVPFVFNPGIVIVAFLFSAAVGVIFGYFPAFKAAS
                     LDPIEALRHE"
     gene            complement(2949..4769)
                     /locus_tag="PLE88_11875"
     CDS             complement(2949..4769)
                     /locus_tag="PLE88_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77204.1"
                     /translation="MKKKHWINYGFKVQEKSSKGHLCSPFVLSFALFVTGISAKADSY
                     RDLYADTWAAADALGRQLPGYEECGPPKENKKVGIFYFLWLGQHSKDGPYDITRLLKE
                     NPENPSWGPVGNFHHWGRPELGYYTSDSVYVIRKHLRLLSDAGIDVLIFDVTNTYTYP
                     NIYSIICEEMTNLRSGGQHTPQIMFLTHSQAGATITTLYRDFYSQNRYPDLWFYWKGK
                     PLILGKSSELDSEIRNFFTMRDCWAWTHGRDTWQWLDHWPQQFGWHESPDKPEELSVC
                     AAEHPISNIGRSHYRGKQPPTNYYGVASQTDRGLYFRQQWERALEADPEFIFITGWNE
                     WAAQRFLKEEGQPPAEMAGRPLRPGDTYFVDAYTQEYSRDIEPMEGGHGDNYYYQMVA
                     GIRRYKGVRPPPPVSAKKTISVDGEFDDWEDVTPEYRDHVGDVEHRRETGWGNAGMYV
                     NMTGRNDFVTMKAARDDQNLYFYAQTSQPITPHTDSNWMLLFLDTDQDSRTGWHGYDF
                     VVNLTVRDNTRTSLHSLASGWNPQPAGWIPYAARGRCLELSIPRELAGCKAGDVKLDF
                     HWADNLQKLGDIEEFALNGDSAPERRFNYRYASSELDGLK"
     gene            complement(4816..5220)
                     /locus_tag="PLE88_11880"
     CDS             complement(4816..5220)
                     /locus_tag="PLE88_11880"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM77205.1"
                     /translation="MFQLDNMSLLCELLSTEEDNLWDYTLPDGRNMQKAMEFLFPYLK
                     DKSKWPYGADIEHFEDWPVRQPCLLLGGYAFGRPDYLALWRTLNPDPTNLEVQRNMAV
                     TQPLLWLLRDEDIPLERLRIERTRSEGSVEFE"
BASE COUNT         1426 a         1420 c         1412 g         1371 t
ORIGIN      
        1 aaaggtattg cagaccaagg cgaccgtaag tgaagcccgc cttaaacttg aaaagtatga
       61 acatgttcgc cgattgagcg ataataaagt accctctcag agcgaatttg atgcggccca
      121 ggcggcctat gatcgagctg tggcggatga ggccagcgcc gaagccgctg aagcacaggc
      181 acaagccacc cttgaggcgt accagacaga tctgtcaaag gccgttattt actctcccat
      241 taacggaatc gttctcaccc gcagtgtgga acccggccag actgtggccg catcctttac
      301 cacgccggta ttgtttacgc tggcggagga cctgacaaag atggaattgc atgttgatgt
      361 ggacgaggcg gacataggcc aggtcaaaga aggacaaaac gcaaccttta cggtggatgc
      421 ttatccgaat caaagtttca atgcgcagat cacacaggtc agatacggct ccaccacggc
      481 aagcggtgtc gtaacttatg aaaccgtgct tgaagtcgct aacaatgatt tatcgctgag
      541 gccgggaatg acggcaaccg ccgatataat agtgataagg gccgaagata tccttaccat
      601 acctaactcg gccctgcgat ttaaaatggc agaagcggcc gaagcccaaa acaccaacgg
      661 aagcattgtc gattctctgc ttcctcatcc tccaaagcac gaatcaaaac agcaggaaac
      721 ggctatagtc agcggcagca cgcagcagat ttatgtattg aaagaaggca agcccgctgc
      781 tgtctcgata acaacagggg ttacagacgg cattaacaca caagtgcttg atggtgaaat
      841 caaagaagga atgccggtga taattggaac aatagcccaa gagaaaaaaa tatgatccaa
      901 tcagcataca gcggcaataa tcagaatgtg attgatctgc aggatataaa gaaaatctac
      961 ggacgcggcc aggcggtaat gcaggctctt cgcggaatca gtttgaaaat tgagagaggc
     1021 gatttcgtcg cgataatggg ccccagcggc tcaggaaaat caacctgtct taatattctt
     1081 ggctgccttg atgcgccgac cgagggcgat tatatattca acggcatcag agtcaacgag
     1141 cttgaccgta atcagcgtgc gctgcttcgc cgccattacc tcggctttgt tttccaggga
     1201 tacaatcttc tgaatcgaac ttcggccctc gaaaatgttg aactgcctct gatctatcgc
     1261 ggcatcggcg taaaggaaag gacaaaacgt gctcgccagt cgcttgaaaa agtagggctg
     1321 ttggaatggc agacgcatac tcctggcgaa ctgtccggcg gccagcagca gcgggttgcc
     1381 attgcacggg ctattgtgac ggaacctgaa gtattgctgg ctgatgaacc aaccggaaat
     1441 ctggatacga ccagaagcca cgaaattatg gaccttctgg ttaacctgaa tcgcgacctg
     1501 ggaattacta ttgtaatggt tacacacgaa ccggatacgg ccgcatatgc aaaacgcgtc
     1561 attcattttc tggatggcct gatagcgtca gataagaaaa atggggagtt aaaataatgc
     1621 tgctgtggga tacgcttcta atcgcgctgc gtgaaatcag gcggcacatt ctgcgttcat
     1681 tcttaacagt tcttggcatc gtcattggtg tggcatcggt aatcaccatg gtaactcttg
     1741 gaaacggcgc tactgttcag gtaacccagc aaatcaagag tatgggaagt aatctgctta
     1801 tggtcactgc tgggaaaagg cttggtcctg gccgtgattc atccgctccg tcgttcaaaa
     1861 ttgcagatgc cgaggctatt gccagagaca ttagctcaat cgacgccgta gcaccggctt
     1921 cgagtcaatc cgtaaccgcg gtattcggaa atgaaaactg gtcaaccact gttacgggaa
     1981 caaccaatca gtatttacgg gctcgaaatt gtgcaatcca gacaggccgt gaattcagca
     2041 ccagcgaatt acgcgcaggg gcggccgtat gcattatcgg agatactatc cgaaaaaagt
     2101 tgttcggccc gcaagaccct gtgggcaaaa gattgcgact cggaaaactt tcttttcagg
     2161 tagtcggttt gctcgaagca aagggacaaa gctcaatggg gcaggatcag gatgatattg
     2221 tcctaatccc catcaaatca tttcagcggc gtctttccgg gaatacggat gtcggcataa
     2281 tactggtgtc cgccagtgat aatgcttcta cagaggccgt gaaggaagat attgaaaatc
     2341 tgatgcgaga gcgccgtcat atttcaaaaa acgaagagga caattttaat gttatggata
     2401 tgacggaaat ctcgaacatg cttacaggaa ccactcggat tctgacggct ctgcttggag
     2461 cggtggcggc ggtcagtctc gtcgtcggcg gaatcggtat tatgaatata atgcttgtat
     2521 cggttaccga gcggaccagg gaaattggta ttcgtcttgc aatcggtgca ctcgaacggg
     2581 aggtgctgat gcagttcttg gtggaggcgg tagtgctgtc gtctgccggc ggtcttctgg
     2641 ggattgtagt tggtttgttg atgtgcgcag gtctcaaagg ggttctgcag gttccctttg
     2701 tgtttaatcc gggcattgtc attgttgcat ttctgttctc tgccgccgta ggggtaattt
     2761 tcgggtattt ccccgcattc aaggccgcaa gtcttgaccc catcgaagcc ctgcgccatg
     2821 aatgatttac gatatttgcg gctgtcatca gcgtcaaaca taaattccct ttgatggcgg
     2881 atattcttgc ccttacgtcg gttattgtaa gagctggtat aggtacgccg gacagggcat
     2941 aatcattttc attttaagcc atccaattct gacgaagcat agcgatagtt aaaccgtctt
     3001 tccggagcac tatctccatt gagggcaaat tcttcaatat cccctaattt ctgcagattg
     3061 tcggcccagt gaaaatccag tttgacatct cccgctttac agccagccag ttctcgtgga
     3121 atcgaaagtt ccagacatct gccccttgcc gcatagggaa tccatcccgc cggttggggg
     3181 ttccagccgg atgcaaggga atgaagactg gtcctcgtat tgtctcttac cgtcaggtta
     3241 acgacaaaat catatccatg ccaccctgtt ctggaatcct gatcggtatc caaaaacagc
     3301 agcatccagt tggaatccgt atgcggcgtt atcggctggc ttgtctgcgc ataaaaatag
     3361 agattttgat catcgcgggc agctttcatg gttacaaaat catttcggcc ggtcatattt
     3421 acatacatcc ctgcattacc ccaacctgtt tccctgcgat gttccacatc cccgacatga
     3481 tcccgatatt caggggtcac atcttcccaa tcatcaaatt cgccatctac ggaaatggtt
     3541 tttttcgctg agacaggcgg gggcgggcga acccccttgt atcggcgaat tccggccacc
     3601 atctggtaat agtagttgtc cccgtgaccg ccctccatcg gctcaatatc ccggctgtat
     3661 tcctgcgtgt aggcatccac aaagtaggta tctccgggtc gcagcggccg gcccgccatc
     3721 tctgccggcg gctgcccctc ttctttcagg aaacgctgag ccgcccactc attccagccg
     3781 gtaataaaaa taaactccgg atccgcttcc agagcccttt cccactgttg acggaagtaa
     3841 agccctctgt cggtctgact tgcaaccccg taatagttag tcggaggctg cttgcctcgg
     3901 tagtggctgc gcccgatatt ggagatcggg tgttcggccg cgcagacgct gagttcctcg
     3961 ggcttgtcag gcgattcatg ccagccgaac tgctggggcc agtggtccag ccactgccag
     4021 gtatcccgtc cgtgggtcca ggcccagcag tcccgcatcg taaagaagtt tcggatttcg
     4081 ctgtccagct cgctggactt tccgagaatc agcggcttgc ctttccagta gaaccacaaa
     4141 tcaggatagc ggttttggga gtagaagtcc cggtacagtg tggttatcgt tgctcccgct
     4201 tgggagtgcg tcaggaacat aatctgcggc gtatgctggc cgcccgatcg cagattcgtc
     4261 atctcctcgc agatgattga ataaatattg ggataggtat aggtattggt tacatcaaaa
     4321 atcagcacat caatacctgc atccgacaga agccgaagat gttttcgaat cacataaaca
     4381 ctgtcgctgg tataatatcc cagttccggc ctgccccagt ggtgaaagtt gccgacaggg
     4441 ccccacgaag gattttcggg gttctccttt aacagccggg tgatatcgta aggaccgtcc
     4501 ttggagtgct ggccgagcca gaggaaatag aaaatgccga ctttcttatt ttccttcggc
     4561 ggtccgcact cttcatagcc aggcagttgc ctgcctaagg catcggcggc cgcccaagta
     4621 tctgcataca gatctcgata ggaatccgcc tttgcagaaa tccctgttac gaataaagca
     4681 aaagataaaa caaaggggga gcataaatgc cctttggaac tcttctcttg cactttgaat
     4741 ccataattta tccagtgttt cttcttcaaa atgtccactc catcaaaagt tcccatattt
     4801 gcggcagaga cgggtctatt caaactcgac gctcccttcc gaccgcgtcc gctcaatccg
     4861 cagacgttcc aaaggaatat cctcgtctct gagtaaccac agcaggggct gggtaaccgc
     4921 catattccgc tgcacttcca aatttgtcgg atcaggattc agtgtcctcc acagtgccag
     4981 ataatccggc cgcccaaaag catatccgcc cagaagcagg cagggctggc gaaccggcca
     5041 gtcttcgaaa tgttcaatat ccgcgccata gggccatttc gatttatctt tcagatacgg
     5101 aaacagaaat tccatcgctt tttgcatatt tctcccgtcg ggcagtgtgt aatcccagag
     5161 attgtcctct tcggtcgaaa gaagttcaca cagcagactc atattgtcca attgaaatat
     5221 ggaatagctg tatcaaagcg agctgaatgc cggatatacg gataatccag gacaaagcca
     5281 tcgacggcgt atgccggtac cgcatcagcg taatagttgc tggccctcgt cggcttgtgc
     5341 aaatacgaaa agttttgaaa aatggctgtt ttctcaatct cgaagacgca caaagggggt
     5401 agtgtttttt tttgtgtctt aaagaagtta ggaaatatcc gctgttatcc gccctttaat
     5461 acgacaagcc atatccatat tcaaacaacg gctcgtatgg ctgatctcca atattgatcg
     5521 gaatctgctc catagacttg ggccatgtaa acgaaagttt tccagtcggc ttataatccc
     5581 ccaaaagaac atccgcaatc ccctgtccct ccgtgccggg cagccaggc
//
LOCUS       DAOTBO010000142         5627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12077_length_5627_cov_5.979541, whole genome shotgun sequence.
ACCESSION   DAOTBO010000142 DAOTBO010000000
VERSION     DAOTBO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12077_length_5627_cov_5.979541"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLE88_11885"
     CDS             complement(<1..934)
                     /locus_tag="PLE88_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOM77206.1"
                     /translation="MMKTQKVRQNNLIAFLSWSLLTALFAAAYWMASQTQTLGHYRKW
                     LGGAGLACLSIAAFNLSMLLIGYYIVRNKRPVVEISMLSKPLKLVTAAIVLFIVFYCL
                     GKLSAFGTFFSLFGGLLLGWSLQTPLSGLVAWFLICIKRSIRPGDRIQFPDLKLTGDI
                     EDVGAMYLTLNQVGGSITSEEAVGRRVLVPNAMLFNYVVINYTVAQEAAYILDEVVVR
                     ITYNSNWEKAEEILLDAARVVTADIIEATGTQPYIRSSLYDYGIYLRLRFKTRVKDRA
                     EIAYKIEKKICETIQKTPSVDIAIPYVYSYRTAMV"
     gene            complement(1312..1542)
                     /locus_tag="PLE88_11890"
     CDS             complement(1312..1542)
                     /locus_tag="PLE88_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77207.1"
                     /translation="MLKWQITMVLYGENRKKTFTVCADGLNQVKRRTLQEFRKLAPQK
                     KNLYLEVKQPGIYAIVSNLDDVGEVMLERVVQ"
     gene            complement(1599..2141)
                     /locus_tag="PLE88_11895"
     CDS             complement(1599..2141)
                     /locus_tag="PLE88_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOM77208.1"
                     /translation="MTRCNQNIIQHSITNAVPPARGGFLRRIYRYLKLFTYSRGRSAH
                     LKRFLERVRGRQSRILLSENDYYRLLAMLRKKDSASASKRAKLRYLLRKIDLRPFRLV
                     PNDVITMNSSFVILSSRGTKYKLQLVYPDSADKAQGQISILSWLGMCLLGKRKGDRIG
                     NTLSVGDILYQPENSGHYHL"
     gene            complement(2163..2579)
                     /gene="rnk"
                     /locus_tag="PLE88_11900"
     CDS             complement(2163..2579)
                     /gene="rnk"
                     /locus_tag="PLE88_11900"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOM77209.1"
                     /translation="MQKDTIYMTQPDVEQLNELFEVLKKQNSRDLGFIEPLRREIKRA
                     KVLTPACISNDVVTMNSRVRLKDMATGEELVFQIVFPTNADLDKGKISVLAPIGTALL
                     GYRVGDVVAWPVPGGKRHLKIEEMLYQPEAAGDFFQ"
     gene            complement(2956..4446)
                     /locus_tag="PLE88_11905"
     CDS             complement(2956..4446)
                     /locus_tag="PLE88_11905"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOM77210.1"
                     /translation="MDVVYIVGPMLLIAVVLAAVWLERWSVPVILVALGAGILFGSDV
                     LNLWHFDDIHLANQVANIALVFILFQGGFSTKRETFKTVALPAGGLATWGVMLTAAAT
                     FGVLWGILHWSLEKSILLAVIISSTDAAATFSILRRQSLPSKLASTIEVESAANDPMA
                     VLMTVVAVESFAFGQKAEWYMVLLLFCWKFIVAPLFGWFSGRGMLWLFNRLRPQDRGY
                     YYVLSLGLILLIYGAAELIHASGMLAVFVAGYVMGNHPFVHKQGVTNFSSALSIMANI
                     GMFALMGLLVFPHQWSTVWVDGIILFLVLTFVARPVAVWLGTLGMGISWKHKIFMMWA
                     GLRGAVPIVLATYPATAGMGVGQDIFNLVFFAVLLSILIQGSTLGFVAKLLGLSAPLR
                     PKPLYNLELITMAPSDLDLFVVDLPGPQGTIGPKISDLHLPPGSVITLITRGQEVVVP
                     KGSTHLQGWDQVTVLARAKDEEMVRRSLLEPFILYPTKENTEKRGA"
     gene            complement(4849..5262)
                     /locus_tag="PLE88_11910"
     CDS             complement(4849..5262)
                     /locus_tag="PLE88_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOM77211.1"
                     /translation="MRLFPRRGTASRIFLEIDYYRLQGLLTKQHHDKYRALSVQFRRL
                     RQLLDGGLLYPCKKVPDNLITMNSVIRLRSRRGTEFTVCLVYPREADKSQRKRSVLSP
                     LGLALIGKTEGEMMRPSMTIEKILYQPESVGNYYQ"
BASE COUNT         1540 a         1431 c         1207 g         1449 t
ORIGIN      
        1 caaccattgc tgttcgatac gagtacacat agggaatagc aatatcaacc gacggtgttt
       61 tttgaatcgt ctcgcatatt ttcttttcga ttttataggc gatttccgca cggtccttga
      121 cacgggtttt gaagcgcagc cgcaggtaga tgccgtaatc atacaaactg gagcggatat
      181 acggctgtgt gccggtcgct tctattatat cggccgtaac gacccgggcc gcatccagca
      241 gaatttcttc ggccttctcc cagttggagt tgtaggtaat gcgtaccacg acttcatcca
      301 ggatataagc ggcctcctga gcaacggtgt agttgatcac gacataattg aacagcatgg
      361 cattgggtac gagcactcgc cggccgacgg cttcctcgct agtaatgctg ccgccgacct
      421 gattaagtgt taaatacata gcgcctacat cttcgatatc gccggttagt ttaaggtcag
      481 gaaattgaat tcggtccccc gggcggatgg accgttttat acatatcaaa aaccaggcca
      541 ccagcccgct tagaggcgtc tggagcgacc agcccagcag cagccccccg aataagctga
      601 aaaacgtgcc gaaggccgac agcttgccca gacagtaaaa aactataaac agcacaattg
      661 ccgctgtaac gagtttgagc ggcttactca gcatggaaat ctctacaacc ggtcttttat
      721 tccgcacgat atagtaaccg atcaggagca tgctgagatt aaacgctgcg atcgacagac
      781 acgccagccc agctccgccc agccatttgc gataatgccc tagtgtctgc gtctgtgacg
      841 ccatccagta ggcggccgca aaaagcgctg tcagcagtga ccagctcaaa aaggcaatca
      901 aattattctg tcggaccttt tgcgtcttca tcatcgtaat tcccgtgaag tatcataaat
      961 taaactggtt ataagcttcc ggctgataga tcattttttc gataaacatc tgtctgccga
     1021 tactgtcgcc aacgcgacgc cctaaaatat tcaatcgcag cggcgtcagg attgatacgt
     1081 tgagtaccac ccggcccgtt aattgacggc actcaggttg gctgggaaaa caagagtcag
     1141 gtttaacatc ttcctgcccc gatgatcctg cagcgccacg gggcagaccc gctttcgata
     1201 atgctcgact gacatatcaa ggccttcagt cgttgagcgt caaatacgga cacctcgata
     1261 tccggttgtt atattgcctg ttctatcttt aacctccctt cggtaatgag attattggac
     1321 gactcgttcg agcatcactt ctcccacgtc gtccaaattc gaaacgattg cgtaaatgcc
     1381 cggctgtttg acctccagat aaagattttt tttctgcggc gccagttttc gaaattcctg
     1441 gagtgtccgc cgtttgacct gattaagccc gtcggcacag acggtaaaag tttttttcct
     1501 gttctcaccg taaagcacca ttgtaatctg ccattttaac atcgttaacc ctccgtgaaa
     1561 ggttgtccgt tcattcttca tcgccttttt cgggatggtt ataaatggta atggccgctg
     1621 ttttccggct ggtacaaaat gtctcccacc gacagcgtat ttccgatgcg gtcgcctttg
     1681 cgtttaccca ggagacacat ccccagccaa gaaagaatgg aaatctgccc ctgggcttta
     1741 tctgccgaat ccggataaac aagctgcagt ttgtatttcg tgccccggct gctcaaaatg
     1801 acaaatgagg agttcatggt aatcacatcg ttcggaacca gccgaaaggg tcgaagatca
     1861 attttccgta aaagataacg gagcttggcg cgtttggacg ctgaagccga atcttttttc
     1921 cgtagcattg ccagcaggcg gtaatagtcg ttttcagaca gcaggatgcg gctttgccga
     1981 ccgcgaaccc gctcaagaaa ccgtttgaga tgagccgacc gccctctgga gtacgtaaaa
     2041 agtttcagat atcgatatat ccggcgaaga aaaccgccgc gggcaggcgg tacggcatta
     2101 gtgatagaat gttgaattat gttttggtta cagcgtgtca tgacagattc ctttctttaa
     2161 agttactgga aaaaatctcc agctgcttcg ggttgataga gcatctcttc gatctttaaa
     2221 tgtcgtttgc cgccggggac gggccacgct acaacatctc ccacccgata ccccagcagg
     2281 gcggttccga tgggggccag aacggatatc tttcctttat caagatctgc atttgtcggg
     2341 aacacaattt ggaataccaa ttcttctccg gttgccatat ctttgagccg cactcggcta
     2401 ttcatcgtta ccacatcgtt ggaaatacac gcgggagtaa ggactttggc tcgtttaatt
     2461 tctcgcctaa gcggctcgat aaagccgaga tcacgagagt tctgcttttt caaaacttcg
     2521 aatagttcgt tgagctgttc gacatcgggc tgtgtcatgt aaatagtgtc tttttgcatc
     2581 gtttcgcacc tttcctggag ctattttcgg tttaggtttt tctcaggttt caatttttcg
     2641 agcgcaaaaa aaagggccag ccttgctgac ccgtactgtc ataatgtagt ttaggtcagc
     2701 gcatcatggg acatacccgt aaagacttat aacgcttaca aacatcattg aaacgattgt
     2761 tttcggttgt tgcataaaaa ccctaagtgt ttctaatata ttaagttata tactatctat
     2821 ttaaactatc ttccaaaccg cccataatat agcatataaa aactgttctg tcaaccccta
     2881 aaatcgccac tcgtgatgct tatagaactt cgaggcaaag ccatctacca ataattgtat
     2941 ttcttgcggc atgttttatg caccccgctt ttcagtattt tccttcgttg ggtaaagaat
     3001 aaacggttca agaagagacc gtcgtaccat ttcctcatct ttcgcccggg ccagtacagt
     3061 cacctgatcc catccctgca agtgagtaga acctttgggg acaacgacct cctggcctcg
     3121 ggtaatcaag gtgatgactg aacctggtgg aagatgcaaa tcactaatct tcgggccaat
     3181 ggtaccttgg ggacctggta aatccactac gaaaaggtca aggtcgcttg gagccatggt
     3241 gatgagctcc aagttataca gcggcttagg ccgcaagggt gcggacagcc ccagcagttt
     3301 ggccacaaaa cccaacgtag aaccctgtat caatatcgat aatagtaccg caaaaaacac
     3361 aagattgaaa atatcctgac caacccccat gccggccgtg gccggatagg tggccaaaac
     3421 aatcggaacc gctccccgaa gtcccgccca catcataaag atcttatgtt tccaagagat
     3481 acccatccct agtgttccca gccataccgc caccggccgg gccacaaagg tcaacaccaa
     3541 aaataaaata attccatcca cccaaactgt ggaccattga tgcggaaaca ccagaaggcc
     3601 cattaaagcg aacattccaa tatttgccat aatcgataaa gcagatgaaa aattggtcac
     3661 accctgttta tgcacaaatg gatgattgcc catcacataa ccggcaacaa aaacggccag
     3721 cattccgctg gcatgaatta attctgctgc gccgtaaatt aatagaataa gccctaacga
     3781 tagcacatag tagtatcccc gatcctgagg acgaagccga ttgaaaagcc aaagcattcc
     3841 gcggcctgaa aaccagccga acaatggagc cacaataaat ttccaacaaa acaagagcag
     3901 caccatgtac cattcggctt tctgtccgaa cgcaaagctt tcaactgcaa ccacggtcat
     3961 taaaacggcc ataggatcat tggcggcact ttcaacttct attgtggacg ctagtttaga
     4021 cggcaaggac tgtcgacgca ggattgaaaa ggtggcggcg gcatctgttg atgaaatgat
     4081 tactgccaac agaatggatt tctcaagaga ccagtgcagg attccccaaa gaacaccaaa
     4141 ggtcgcggca gcggttagca taactcccca tgttgcaaga ccgccggctg gtaaagcaac
     4201 cgtcttaaat gtttctcgtt tggtagaaaa accgccttga aacaaaataa agactaaggc
     4261 aatattagca acctggttgg ctaagtgaat atcatcaaaa tgccataggt tcagaacatc
     4321 gcttccaaag aggattccgg ctcccaaagc aaccaggata acaggaacgc tccaccgctc
     4381 aagccagact gccgccagca ccaccgcgat caaaagcatc ggtccaacta tgtatacaac
     4441 atccatgaat tgcctcaaaa tgtctacaaa gctttgacaa gcgatgaccc aagataaacc
     4501 tttggaaaat acatcactta taccaaatta cgtttttcca catctccaag ctatcattta
     4561 ttcaccttta acgtatatac cgtttttgaa tgaaattgtc cagataacat ttagtgataa
     4621 tcaggttgat gtccgataat gctggtttta atgaatttga attaagcttg ctggatgtgt
     4681 tctcttgaaa gggttatatc ttcaactgcc gtagaggata ttggatattt agaggatgcg
     4741 caatattgac atgtccggca agataaggtt ctattactct ctcccacaca aaacggcagc
     4801 tcaccggttt tctaaggcca cttatattga aacctccgcg tagaaaagtt actgataata
     4861 atttcccaca ctttccggtt gataaagaat cttttctatc gtcatactcg gccgcatcat
     4921 ttcgccctcg gtcttaccga ttagcgcaag tcccagcggg gataacaccg acctcttccg
     4981 ttgcgatttg tccgcttcac gaggatacac cagacatact gtaaactccg ttccccgccg
     5041 cgaacgcagt cgaatgaccg agttcatcgt aatcaggttg tctggaactt tcttgcacgg
     5101 atacaacagc cctccatcca acagttgccg caaccggcga aactggaccg acaatgcgcg
     5161 atatttatcg tgatgctgtt tggtcaaaag cccctgcaag cgatagtaat caatttccaa
     5221 aaatattcgc gatgccgtgc cccgacgtgg aaacaaccgc atccgtaaag actgccaaaa
     5281 gctttttggc ttatcggatt ttatcatctg aaccatactg acattgtcca tttatcaaag
     5341 tctatcgtcc accagccgat attggttaat acaaagtatc ttccgtgcta aatggcttca
     5401 tgcccgtagt cacctatcct tgaacatggc cgcagacgct gataaccagc aacaccatcc
     5461 cgaccgaaat ctgcacctgc aatttgcatt gtcctgactc aacattcttc ggatattaca
     5521 agctgatatg tcattttact caatgacgat agacatacct gagcatgtct ttagggaacc
     5581 tctaataatt tgatttttga ggatcaaaat ctcagttaac gtttttt
//
LOCUS       DAOTBO010000143         5584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12197_length_5584_cov_8.196057, whole genome shotgun sequence.
ACCESSION   DAOTBO010000143 DAOTBO010000000
VERSION     DAOTBO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12197_length_5584_cov_8.196057"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4393
                     /locus_tag="PLE88_11915"
     CDS             <1..4393
                     /locus_tag="PLE88_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOM77212.1"
                     /translation="QGGVGNISQNPHFRDPTEDDYRLLLSSPCIDAGDPASEYLNEPS
                     PNGGRINMGAYGNTNEAATTVEDRENYSTADDGIPDWWKIKYNLDPLYDIASQQSIGS
                     QGHLTYGEEYLYGTNPNLYDTDSDGLPDWWAILYELDPLDPSNAGQISAGSDGMLTNL
                     EEYWSGTNPNLLSTVDDDIPDWWKIKYGLDPLAPAIANLDSDGDGLTTLQEYTAETNP
                     SHHNEFGMIIEYDYDTAGRIQKQRVLNGLETKFSVTAYEYDELGRQILQRQYQFNNVT
                     ETYTSNVADNDVVSLTVYDVLGDVQWSVQKVEHSNNPVSLHYDGIVDDPSIVQNGDIV
                     TMQETRYEDVDSSANGMLEQVSETKVGHYASTGTINAGCAITTSITYYDRDDEIPSPS
                     GDNIYCWTESIAPAHDDSGSRAITTSYYDGAGRVFWITDARNHYRTLEYDSYGQVIRE
                     IAWENIADPDPDEPVMQTRSVYDNLGRITRKVTMADAADSDGIDTTEDIVTDYTYDYD
                     YPSGGTERNYPGQLVAQTIYYGSSSTDATTYYDYDALERRYKTTDPVGNTTELFYNAS
                     GQVIRGLQTEMNPGGGSAGLILYNDSYYEYDSAGRIASIIGKPYNDSDELLYDSSDVK
                     HVAGTLPPIPEPLPSSWMLTTFYYDAAGRKIAEVKPNDVKTIYTYWAAGQKKTEVADA
                     EGGVTPDIKQTTEWSYDRVGRLAAIAGYTNESDDGTRQETRYAYNDQGNVTDIYYPEY
                     DENVSITKGRIQYAYFANGQVKQRTDQRGIVTTYAYDATGVNLLTKSATIASVTTTET
                     FMYDAMGRMENANKVEGTTQISRSELGYYDTGYLQSETQKLFGDPTGKTVSYAYDTAG
                     FRTLIGYPNTSPITLTRTNTPLGQIDTLSLDGTTNLVDYDYLGSRVAERTYPDISVSV
                     VYGYDQYGRITDITAGSLVDFHYEYVPKENNIWKMRFDHRSGSPYNEYAYDDIDRLTG
                     VTYLSNGSDTEAFVMDDLGNRAGNQTLRGEGTVGFAVDTLTNRYTSVGGHNLDYDDAG
                     NLTTDKDGYVYTYDYENRIVKIEDDQQTLIAEFAYDTQGRRIKVYDAVAQTTTLYYYS
                     DNWQVLAEYDDSGVQKRYFVYGNYIDEVLFMKDTDASDGTPAMNYYYLHDHLYSPVAL
                     LRKLGGVVLERYEYDAYGKMTRLNPNFTAFSGTEAGNPYYFTGRELDVLDGGGLRPMH
                     YRHRAYDTHAGRFMQQDPLGIQPAANAQINPFKPEKQYTDGMNIYEYVGDGPISSNDS
                     LGLTELHPVRVNRLHCKCKRECGPDITDSFFHGLERVSIALEEEFKKPGRNEAICGNE
                     KFGTGGVFSRTGWDIDQFWGSMFLYDSERVRWPSKKEPCADTVVFKGHCVKKWELNYY
                     LWGYVNRKCNTSWLKYHFYTDLWTEFKSAFSPEDPHCKRSFAGCGQTGALVDNSCYLD
                     KCCKVSGAPIWTNPITMQVADVKMPTE"
     gene            4438..5196
                     /locus_tag="PLE88_11920"
     CDS             4438..5196
                     /locus_tag="PLE88_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOM77213.1"
                     /translation="MDLIKYTNKALFVSLFFLLVVIACFMLAEKKREARLKKEKHLFY
                     AISRNDPELLLQMIREGHDVNTKWNGWSTTDAALKWTRPKVRGKTFILKDLQDWDNKA
                     SSLINIMIQNGLNIKSLDPPPLSFAAYWHLENTVKILLGAGANPNVGTKDYGISPLHG
                     VFIFYDDDWKNNEILKMLVDYGADVNCQDKEGNTPLHLAILYNADISIIKYFLDAGAL
                     PSLQNERGQTAIDIAETSPNPSHPLISLLKEYEK"
BASE COUNT         1632 a         1180 c         1361 g         1411 t
ORIGIN      
        1 acagggcggc gtagggaata tctcgcaaaa tccgcatttt cgggatccca ctgaggacga
       61 ttaccggctg ctgctttctt cgccttgtat tgatgcaggg gatcctgctt ctgaatattt
      121 gaatgaaccg tcgcctaatg gtggtcgaat caatatgggc gcttatggaa atacgaacga
      181 ggcggccacg actgtagaag acagggaaaa ctatagcact gccgatgacg gcatcccgga
      241 ttggtggaag ataaagtata atcttgatcc cttatacgat atcgccagtc agcaaagcat
      301 tgggagtcaa ggtcatttga catacgggga ggaatacctg tacggaacca atcccaactt
      361 gtatgacacc gatagcgatg ggttgccgga ttggtgggcg atcctgtatg agcttgaccc
      421 attggatccc agtaatgccg gtcagatcag cgcaggaagc gacggcatgc tgacaaacct
      481 tgaggaatac tggtcaggca ccaatccgaa tctgctctcc accgttgatg acgacatccc
      541 ggattggtgg aaaataaagt acggattaga tcctttggcc ccggctattg caaacctaga
      601 cagcgacggt gacgggttga ccacacttca ggaatacacg gccgagacca atccctcgca
      661 ccacaatgaa ttcggtatga ttatcgaata tgactatgac actgccggca gaatacagaa
      721 acagcgggtt ctgaatggtc ttgaaacgaa attttctgta actgcatacg aatatgacga
      781 gcttggccga cagattctac agcgtcagta tcaattcaat aatgtcacag aaacttatac
      841 gagtaatgtc gctgacaacg atgtcgtttc attgacggtt tatgatgtat taggtgatgt
      901 acaatggtcg gttcaaaagg tagagcacag caataatcca gtatctcttc attacgatgg
      961 tattgtagat gatccgtcta ttgtccaaaa cggcgacatc gtaacaatgc aggagacgcg
     1021 ttatgaagac gtcgatagtt cggcaaacgg catgctggaa caggtttccg agacgaaagt
     1081 tggccattat gcttcgaccg gcactatcaa cgccggttgc gcgataacca caagcattac
     1141 ctattatgat cgtgatgatg agattccgtc acccagcggc gataatatct attgctggac
     1201 ggaatcgatt gcccctgctc acgatgattc cggttcacga gcgattacca caagctatta
     1261 tgatggggcg gggcgtgtgt tttggattac cgatgctcga aatcattacc ggacgctcga
     1321 atatgacagt tatggacagg ttatcagaga aatcgcatgg gaaaatatag ccgatcctga
     1381 tcctgatgag ccggtaatgc agacacgctc tgtttatgac aatctgggac gcatcactcg
     1441 caaagtgacg atggcggatg ccgccgactc ggacggtatt gatacgactg aggatatcgt
     1501 aaccgattac acgtatgatt atgactatcc aagcggcgga accgagcgga actatcccgg
     1561 tcagttggtt gcacagacga tctattacgg cagttcttcg acagatgcga caacctatta
     1621 cgattacgat gcgcttgaaa ggcggtacaa aacgacagac ccggttggaa atacgaccga
     1681 actgttctat aatgcttccg gccaggtaat ccgcggttta cagactgaga tgaatcccgg
     1741 cggcggcagt gccggtctta tcctgtataa tgattcctat tatgaatatg actccgccgg
     1801 cagaatcgct tcgattatcg gcaagcccta taatgattct gacgaactgc tctacgattc
     1861 ttcagatgtc aaacacgttg ccggcacctt gccgccgatt cccgaaccac tgccgagttc
     1921 gtggatgctg acgacgtttt attacgatgc tgcgggtcgc aagatagccg aagtcaaacc
     1981 aaatgacgtg aaaaccatat acacttactg ggcggcgggt caaaagaaaa cggaggttgc
     2041 tgatgccgaa ggcggcgtaa cgccggacat caaacaaacg accgaatgga gctacgaccg
     2101 ggtaggacgc ctggctgcca tcgccggtta cacgaacgaa tcagacgatg gcacacgtca
     2161 ggaaacccgg tacgcctata atgaccaagg caatgtgacg gacatttatt atccggaata
     2221 tgatgaaaat gtcagtatta caaaaggacg cattcagtat gcgtactttg ccaatgggca
     2281 ggtcaaacag cgtaccgacc agaggggcat tgttaccaca tacgcctatg acgccaccgg
     2341 tgttaatcta ctgaccaaat cggccacaat tgcctccgta acgacgacag agacatttat
     2401 gtatgatgcg atgggacgca tggaaaatgc taataaggtc gaaggaacaa cgcaaatcag
     2461 tagatcggag cttggatatt atgataccgg atatttgcaa tccgaaactc aaaagctctt
     2521 tggcgacccg acagggaaaa ccgtctcata tgcctatgac accgccggat tccggacatt
     2581 aattggctat cccaacacct ctccaataac ccttacgcgc acgaacaccc cgttggggca
     2641 gattgatacg ctttcactgg atggcacgac aaacctggtt gactatgact accttggcag
     2701 ccgggttgcc gagcggacat atccggatat ttctgtgagc gttgtttatg gctatgacca
     2761 gtacggccgg ataacggaca tcaccgccgg ttcgctggtg gatttccatt atgaatatgt
     2821 acccaaggag aacaatattt ggaaaatgcg ttttgaccat cgcagcggca gcccttataa
     2881 tgaatacgcc tatgatgata ttgatcggtt gacaggagta acatatttga gcaacggcag
     2941 cgatacagaa gcgtttgtga tggacgacct ggggaacagg gcgggcaatc agaccctgcg
     3001 aggcgagggg acggttggtt ttgccgtcga tacgctgacc aatcgctata cgtctgtcgg
     3061 cggccataat ctggactacg acgatgcggg caatctcacc acggacaaag acggctatgt
     3121 ctatacgtat gattatgaga accgcattgt gaaaattgag gacgatcagc aaactctaat
     3181 tgccgagttt gcttacgata cccagggccg acgaatcaag gtgtatgatg ccgttgccca
     3241 aacgacaacg ctgtattact actccgacaa ctggcaggtg ctggcggaat atgacgattc
     3301 cggtgtacaa aagcgatatt ttgtctatgg taactatatt gatgaagtgc tgttcatgaa
     3361 agataccgat gcgtcggacg gcactccggc gatgaattat tattatctgc atgatcatct
     3421 gtacagtccg gtggccctgc tgaggaaact cggtggagtg gtactggaac gatacgaata
     3481 cgatgcttac ggcaaaatga cccgtttgaa tcctaacttt accgccttct ccggaaccga
     3541 agcgggcaat ccgtactatt tcacagggag agaattggat gtgctggacg gcggcggcct
     3601 gaggccaatg cactaccgcc accgcgccta cgacacccac gccgggcgat ttatgcagca
     3661 ggaccctctc ggcatccaac ccgccgccaa tgcccaaata aaccctttta agccggagaa
     3721 acaatacaca gacggaatga atatttatga gtatgtaggt gatggaccaa tttcaagtaa
     3781 tgattcattg ggtttaacgg aattacaccc ggtgagggtg aatcgcttgc actgtaaatg
     3841 caaaagagaa tgtggtccgg atattacaga ttcctttttt catgggctgg aaagagttag
     3901 tatcgcattg gaagaagaat ttaaaaaacc tggcagaaac gaagcaattt gtggaaatga
     3961 aaaatttggc acaggagggg tattttcacg aacaggttgg gatatagatc agttttgggg
     4021 gtctatgttt ttatatgact cagaaagagt gaggtggccg tctaaaaagg aaccctgtgc
     4081 cgacactgtt gtattcaagg gacactgtgt taagaaatgg gaattgaatt attatttatg
     4141 gggctatgta aatcgaaaat gtaatacatc atggttaaaa tatcattttt atacagattt
     4201 gtggacagag tttaaaagtg ccttctcgcc ggaagaccca cattgtaagc ggtcttttgc
     4261 aggttgtggg caaacaggag ctttagtgga taattcatgt tatcttgata aatgctgtaa
     4321 agtttctggc gctcctattt ggactaatcc cattactatg caggtagccg atgttaagat
     4381 gccaaccgag tgagattttg aatagctgaa tttatgcaag tatatggata ataaattatg
     4441 gatttgataa aatatacaaa taaagcttta tttgtatctc tatttttttt acttgttgta
     4501 attgcatgct tcatgcttgc cgaaaaaaaa cgagaggccc ggctcaaaaa agagaagcat
     4561 ttattttatg caatctccag aaatgatcct gaattattgc ttcagatgat aagggagggg
     4621 catgatgtaa acacaaagtg gaatggatgg tcaactacag atgctgctct taaatggact
     4681 cgcccaaaag tcaggggaaa aacctttatt ttgaaagatc ttcaggattg ggataataaa
     4741 gcgtcttcat tgattaatat aatgattcaa aatggattga atataaaatc cttagaccct
     4801 ccacctttgt catttgctgc gtattggcat ctggagaaca ctgtaaaaat acttttagga
     4861 gcaggtgcaa atccaaatgt agggacaaaa gattatggaa tttcacctct acatggagtc
     4921 tttatttttt atgatgatga ttggaaaaat aatgagattt taaaaatgct tgttgattac
     4981 ggggctgatg ttaattgtca agacaaagaa ggtaacacgc cacttcatct ggcaattttg
     5041 tataatgcag atatttcaat cattaaatac tttttagatg ctggggcatt accgagtcta
     5101 caaaacgagc gaggccaaac ggcaattgac attgcagaaa cttcaccgaa tcccagccac
     5161 ccattgataa gtttgcttaa agaatacgaa aaataaaact ggatgtgtct tatgcaaaaa
     5221 tacatctgac aactgattca gacgtttggc gagacaggaa catattaggt ttgctctttt
     5281 ataagtaaag attgacggat tatggatggg ttcgacagaa tccaaagtcg aaattctata
     5341 aagggttcct ctaaaaattg acaaatttac gtgcaattgc ttgtaccttt aaatatagta
     5401 agctcattga caattaaatc acagtatttc gattcacgga gatagtttcg atgatgcaga
     5461 caggactttt tgactggcaa acacggtttg aacaactgga taacggcggc gatccactga
     5521 caaagcttaa cggtaacatc ccccattttt aggtccattg actaagttag gatttccctg
     5581 taaa
//
LOCUS       DAOTBO010000144         5558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12271_length_5558_cov_4.017808, whole genome shotgun sequence.
ACCESSION   DAOTBO010000144 DAOTBO010000000
VERSION     DAOTBO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12271_length_5558_cov_4.017808"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..475)
                     /locus_tag="PLE88_11925"
     CDS             complement(11..475)
                     /locus_tag="PLE88_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77214.1"
                     /translation="MKALTNSTKSFGENVTKRAILFLAIFFWLIICSIAHGSIGLTVN
                     GDDPVVSPLELKASKSLEIGVIDSDSTETSYDLTLSATGGVFLCEGPTSKSVASSTLL
                     QNETVDIQTSDLSNIGKIYFEFQYDPGVAIVSLTTNQAVNFRIFNNVLCGWS"
     gene            1240..3123
                     /locus_tag="PLE88_11930"
     CDS             1240..3123
                     /locus_tag="PLE88_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM77215.1"
                     /translation="MDNGVFHRIKSFSILGGFLDGLVLNFNTGLNCLIGHRGTGKTTV
                     LEFVRYALDSFPNGEKGITSRKRVESLVKNNLGDGRIRLTIETKDGLEYIIDRTASGD
                     PMVLNADGTPTDISINTGGIFNADIFSQNEVENIADNPQSQLSLIDNFRAAEISEINA
                     QIAMLKSQLQANANEIIPLQKNLTGLAEELNTLPGIEAKIAALAGPKDENTAHVNKAH
                     TDRALREREQQTLTELSELLGKYTDWFGSNGGKFTRQVNNIITQDIMQGSNGQIFQDI
                     QVELVALGDHLDALYNQVTSAIQSQQHSLEENRQKLSQIHDQQELAFRDLITKHTQAQ
                     GVATERANWEKKRNDLLVKKRKSQQFQEQLQQLQTKRTELMGQLQAMYNQRFDIRKVV
                     AQWITENVSSPIRVQVDQFGDRSEYQNLLRDSLKNIGVQQGVVAKKIADLLPPSELVS
                     ILETGTVEDLQNGAELSSSQAQKVFGALKSSDTFMTLDVVELADLPQIELKDGDEFKN
                     SLTLSTGQKCTTILPILLLDSDNPLLVDQPEDNLDNRFIYDTVVDSIRRVHNRRQIIL
                     ITHNPNIPVLGEASQVFVLTSNGQHARILNHGNVDDCKEEIINLLEGGRDAFVQRKQR
                     YNY"
     gene            3123..4277
                     /locus_tag="PLE88_11935"
     CDS             3123..4277
                     /locus_tag="PLE88_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM77216.1"
                     /translation="METEITLTQWPRLTKQLQENPESVPVVVRQQLADSMGQELLQGT
                     IACNETLFLELAEQLTMDPHWQVRLAVSRHLYLFDPAECTDLIVRLCGDDNAYVRKNA
                     ERSLNRQVKFNQKRSKEGKLSKDYLVQVHKLTSQYGPDVANTMFQLAEYRYSLLASTL
                     AHDFRSILTTLSANTEVLLTEHPRHKRAKSIRDDLVLLKQIIENMEQFVRPLNVEKHY
                     ENVDEIIEAAVEKAQIKIAKLGYDISCVQVEIIKPERIRIKVSRPLLLLGLTNLIRNA
                     YESFASDRQGLKEGTIRIEVAVRGYEIQIILKDDGAGIEPQILDKLRTFMPIDINKTK
                     RESTGFGVCIAYRYVVANNGTFEIDSQVDAGTTITMTFPLRDTDAQEENQ"
     gene            4274..5377
                     /locus_tag="PLE88_11940"
     CDS             4274..5377
                     /locus_tag="PLE88_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOM77217.1"
                     /translation="MKILVVEDDHKIKTEVIDDVLASLGYENDWATNQQQAISLLEND
                     NFDLVLLDLQIPARPGSSLASSEFGKHLLQQIREIKGHGNVPVVIMTGHHHDGLDMAS
                     DLIGMGAITFIPKPFPSKGRTLASVIEKTLQQYQKSKELQPPKPEGTFTGGEMVFYPN
                     HIELCGLKILGSKAAPSREILKVLAEIKPDGTYKNWGGEQIADKISAENGIGTVTGCA
                     RTIRTRVAGTLKKHMNVEVGENDFLEHSDQGYRLNHRITVRFCDTHEILLGQNKNLRG
                     QNSVPKGHLRGQKNDPINLGVSYRVKTRCQWILDALNQGQQLRREDVENGLKCSDKTA
                     QRALDVLRNADMIKFQGSKKTGYYVLTKTGNLR"
BASE COUNT         1644 a         1259 c         1255 g         1400 t
ORIGIN      
        1 gctggaagcg ttagctccag ccgcataaaa cgttattaaa aatgcgaaag tttacagcct
       61 gattagtcgt caagctaacg attgccacac cgggatcata ctgaaattca aaataaattt
      121 taccgatatt gctgaggtcg gaagtttgaa tgtcgactgt ttcattttga agaagtgttg
      181 atgaagcgac cgatttactg gtcgggcctt cacacaaaaa aacaccgcca gtagctgaca
      241 gtgttaaatc ataactcgtt tccgtcgaat cggaatcgat cacgccgatt tccaatgatt
      301 tgctggcctt cagttccagc ggtgaaacga ccggatcgtc tccgttgacg gttaaaccaa
      361 tacttccatg agcaattgaa caaataataa gccagaagaa aatggccagg aaaagtattg
      421 ctctcttcgt cacgttctct ccaaaggatt tggtgctgtt agttaatgcc ttcatagtta
      481 cctccgattt cctggttttg atgtaaaact ccagttaaaa accgatctta tgcttatttt
      541 tatgcccgtc aagctatttt ttttgttagc tattgttcca aataatccgg gccggaaagc
      601 cctctaccat attatcttcc tcgcccggca cacgccagaa atcaatgaaa gatagttttt
      661 gtccaagcac ggcaaggctt aaccgcaaca cataagcagc gctccggttt ggagcactga
      721 attctgtgtt tgcggtaaca attttgccgg ttttggaacg aaacaccaaa gtccagtgga
      781 ctttcaattt tcattggtaa tcacatgaaa ctccctattg tcggtcaatg aaaatctgtt
      841 ttctatgatt tcaaaccatc aaaaacaacg attttatcca caaccatttt attcgaatag
      901 ggttacactt cagtctcgaa aaataaaaca gttctctcgg accaaaatca gtccctatat
      961 atataagagc gtttctcaaa gccaaaattt gattataaaa ttggaaaaat ttaaaaatat
     1021 tttcaatgtc cgtatttacc acgtgatttt ggcacatatt accaccaaaa tcctgcatga
     1081 aatttatcat ttcccggttg acaatttccc ggctctggat taaattccat tctgtagtaa
     1141 ggttcagggt ggaaccgaga tctgggttat ccatctccct ccgaagccct cctaaacaat
     1201 ttcgtttaac tcctttgaaa ggaatgggtt atgtcatttt tggacaacgg cgtttttcat
     1261 cgcatcaaat cgttttcgat tcttggtggt ttcctggatg gtttagtcct taattttaac
     1321 actggattga actgccttat cggtcaccgc ggtacaggca aaacaaccgt tttggagttt
     1381 gtgcgatatg ccctggactc cttccccaac ggtgaaaaag gcatcacctc cagaaaacgt
     1441 gtcgaatccc tggttaaaaa caatctgggg gatggacgca tccgtctgac tatcgagacg
     1501 aaagacggtc tggagtatat catcgaccgg accgccagtg gcgatccgat ggtcctcaat
     1561 gcagatggaa cacccactga tatttcaatc aacactggcg ggattttcaa tgcggatatt
     1621 ttcagccaaa acgaggtcga aaacatagcc gacaatcctc aatctcaatt atccctgatt
     1681 gataatttcc gagcggctga gatttccgag attaatgctc aaatcgccat gctgaaatcc
     1741 caattacagg ccaatgccaa tgaaatcatt cctctccaaa agaatctgac gggactggct
     1801 gaggaactca atacgctgcc aggcatcgaa gccaaaatcg cagcattggc aggccctaag
     1861 gatgaaaata ctgctcatgt caataaggct catacggatc gtgctttacg ggaacgcgag
     1921 cagcaaacgt tgacagaact ctcagagcta ttgggtaaat acacggattg gttcggcagc
     1981 aatggaggga agtttacccg tcaagtcaac aatataatta ctcaggacat tatgcagggt
     2041 tccaacggac agatattcca agatattcag gtggaattgg tcgctctcgg cgaccatttg
     2101 gatgctcttt ataatcaggt aacatccgcg atccaatctc aacagcattc cctggaagaa
     2161 aaccgtcaaa aactgtctca aatccatgat caacaagaac ttgcattccg agacttaatt
     2221 accaagcaca ctcaagctca aggagtcgcc acggaacgtg ccaactggga aaagaaacgc
     2281 aacgacctgc tcgtcaaaaa acgcaaatcc caacaatttc aggaacagtt acagcaactg
     2341 caaacaaaac gcacagagtt aatgggacag ttgcaagcaa tgtataatca gcgttttgac
     2401 atacgtaaag tggttgccca gtggattact gagaatgtgt cgtcgccgat tcgcgttcag
     2461 gtagatcaat ttggtgatcg gtcggaatac cagaatctgc ttcgcgattc cctgaaaaat
     2521 attggtgtcc agcaaggcgt ggttgccaag aaaatcgccg atctattgcc gccaagtgaa
     2581 ctggtctcca ttctggaaac cggaactgtc gaagatctgc aaaacggggc tgaactgagc
     2641 tcatcacagg cccagaaagt gttcggagca ttgaaatcat cagatacctt catgactctg
     2701 gacgttgtgg aactggcgga cttaccgcaa atcgaactga aggatgggga tgaattcaag
     2761 aattccctga cgctttccac cggccaaaaa tgcaccacca tattgccaat tttgctgctg
     2821 gatagcgata atcccttgtt ggttgaccag ccggaggaca atctggataa tcggttcatt
     2881 tatgataccg tggtggacag tatccgaagg gttcataatc gccgccaaat tatcctgatt
     2941 actcataacc cgaatattcc ggtcttgggg gaagcatcac aggtctttgt actcacatcc
     3001 aatggccaac atgcccgcat cctgaatcat ggcaacgttg atgactgcaa agaggaaatc
     3061 atcaacctgc tcgaaggcgg ccgggatgcc tttgtccagc gaaaacagag gtataactac
     3121 tgatggaaac cgagataacc ctcacccaat ggcctcgact gacaaagcag ttacaggaaa
     3181 atcctgaatc tgtgcccgtc gtggtacgtc agcagctggc cgactcgatg gggcaggaac
     3241 tattgcaagg aactattgca tgcaatgaga cgctttttct ggaactggcc gaacaattaa
     3301 caatggaccc acactggcag gttcggctgg ctgtgtcacg gcatttatat ctttttgatc
     3361 cggccgagtg taccgatttg attgtcaggc tctgtgggga cgataacgct tatgttcgta
     3421 aaaacgccga acgaagtctg aatcgccagg tcaaatttaa ccagaaacgt tccaaggagg
     3481 gcaagctgtc caaagactat ttggtccaag tccataaact gaccagccaa tacggccccg
     3541 acgttgctaa tacgatgttt cagcttgcag aatatcgata ttctctactc gcatcgacac
     3601 tggcacacga ttttcggagt attttaacaa cccttagtgc caatacagaa gttcttttaa
     3661 ccgagcatcc ccgacacaaa agggccaaat ctattcgaga cgacctggtt cttctgaaac
     3721 agatcattga gaatatggag caatttgtcc ggcctttgaa cgtcgaaaaa cactatgaaa
     3781 atgtggatga gattattgaa gcggccgttg aaaaagcaca gatcaagatt gccaagctgg
     3841 gctatgacat ctcatgcgtt caggtggaga taattaaacc ggagcgaatt cgaatcaagg
     3901 tttcccgacc actgctatta ttgggcttaa caaatctaat aagaaatgct tatgaatctt
     3961 ttgcatcgga tagacaggga ctcaaggaag gaaccattcg tattgaagta gcagttcggg
     4021 gttacgaaat acagattatt ctcaaagacg acggggcggg aatcgagcca cagattttgg
     4081 ataaattgcg cacatttatg cccattgata tcaataaaac caagcgggaa agcacaggct
     4141 tcggcgtgtg tattgcctat cgatacgttg tggccaacaa tggcaccttt gaaatcgaca
     4201 gtcaggttga tgcgggaaca acgataacca tgacttttcc gttgagagac accgatgcac
     4261 aggaggaaaa ccaatgaaga ttttggtggt agaggacgac cataagatta aaacggaagt
     4321 gattgatgat gttttggcct ctttgggata tgagaacgac tgggcaacca atcaacaaca
     4381 agcgatatcg cttcttgaaa acgacaactt cgatctggtt ctgctggacc tgcaaatccc
     4441 ggctcgcccg ggcagcagtt tggctagttc agagttcggt aagcatctac tccagcagat
     4501 tcgtgaaatc aagggacatg gaaatgttcc cgtggtaatt atgacgggtc atcaccatga
     4561 cggcttggat atggcctcgg atttgattgg catgggtgcc atcaccttta tccccaagcc
     4621 attcccttcg aagggccgga cccttgcctc tgtgatcgaa aaaacgctcc agcaatatca
     4681 aaaatccaag gaactccagc cccctaaacc ggaaggtaca tttactggcg gtgaaatggt
     4741 cttctacccc aatcacattg aattatgtgg ccttaagata ctcggcagta aggccgctcc
     4801 ttccagagaa atactcaaag tccttgctga aataaagccg gatggaactt acaaaaattg
     4861 gggtggcgag cagatagcag ataaaattag cgccgaaaac gggattggaa ctgtcaccgg
     4921 atgtgcccga acaatacgta ctcgagttgc cggaactctg aaaaaacata tgaatgtcga
     4981 agtcggcgag aatgattttc ttgaacactc cgaccaaggg tatcggctca accatcgcat
     5041 cacggtcaga ttctgtgata cgcatgaaat tttattgggt caaaataaaa atttaagggg
     5101 tcaaaattct gtccctaaag gacatttaag gggtcaaaaa aatgacccta taaatttagg
     5161 ggtcagttat agggtcaaaa cacgctgcca atggatactg gatgccttga accaagggca
     5221 gcaattgcgg cgggaagatg tagaaaacgg tttgaaatgc tcggacaaaa cggcccaacg
     5281 ggcacttgac gtcctgcgga atgctgatat gattaagttt cagggttcga agaagactgg
     5341 gtattacgtg ctaaccaaga ctgggaatct tcgataagtt cttgaaataa agtattttgt
     5401 gataaatatt cgtgactggc tgcgatgaag tcctggacca agggttcaaa cgattggcag
     5461 tcaccccgag tcttcctaca atcgggccgt tttgacaaaa agtaaaacgg ctggtacact
     5521 gaaaccgccg ctgagcattg agcggtacct ttcctgct
//
LOCUS       DAOTBO010000145         5500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12434_length_5500_cov_5.096970, whole genome shotgun sequence.
ACCESSION   DAOTBO010000145 DAOTBO010000000
VERSION     DAOTBO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_5500_cov_5.096970"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="PLE88_11945"
     CDS             <1..2172
                     /locus_tag="PLE88_11945"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOM77218.1"
                     /translation="VTVTAIDDNFSEGVHTGLITHRSSSVLADYDGLNLPSVEFEIAD
                     NDLPVMFVSNVFVGGQEGPQGTSNYRIPSMTVAPDGSILAFAEGRRNSADPGAVLPID
                     MVMKRSVDNGQTWQPLVVLHHNSSFDYSDPRPVTDLLTGKIHLLYTQWPNLCGQSCVP
                     AGLGDNSSVVFLQTSEDNGLTWSGPINLNTQVKNPTWKALNSGPGHGIQLRWQTNPTR
                     NGRLLAPCHINGVDAVSVYSDDGGLSWQSGTVDTTAPSLNESDVVELTNGDLLWDARQ
                     GSGLYRYRLKSTDGGQTWQYLGLGDIYITPVDCGIDRYSARRDGHDRDRIVFSGPLGS
                     PIGTPGRYNLAVWTSYDEGKTFINPIQINNGFAAYSDIKRLANGRIGVIYEETGSTLI
                     RFASCNIDVFENGPHDPALTQYDGFGNAVDIRRGGVGWTGSWTGLGSFTRSACAAFRG
                     SSIPFERYRFTNQPGRVDSVAGSGMHITRLLNSPFDMHDNGTIYLSLLVSRALDTTAN
                     NDSEEDLNIMLQDSSQQPYISFGVNSREAFYVGKPGSVITTADNAVAMDCSYLMIAKI
                     ITKPDQDDRIYLKVLRSGADAVAGQEMDIDWTLIGASGENAAAVLDRICITAGANAVW
                     SVDEVRIGTTYYSVAGRPDCSQSESYLPGDINRDCYVNLNDLVLLVNDWLSPGCSEQG
                     IYPQIDINGDCDVNMDDMAGLAAGWLDCTDPYNPTECRSVN"
     gene            2242..>5500
                     /locus_tag="PLE88_11950"
     CDS             2242..>5500
                     /locus_tag="PLE88_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOM77219.1"
                     /translation="MTIYKSTRVLGEHSEWDNQDDSMTFQLIVRILLLLIPSACGYTS
                     NIASYNYTSSLGADPTTQGWTYLTNVTNNVYLSGYDASIYPGQSGWRIVDGTSTGYAN
                     YQITLTDDQTEAMKTGFSVSWTFKLDATAWKNDGTGSVTNYYLPPYQSRQNNNGLWIE
                     TAGENSFRYYIVATADASGNLAINDGTVTHVLTAGGGNNGYDTLYTVTVAYDGTKAVL
                     SYGGSDFTLTKQATFGNNRVLFGAHTSPGQGSVVYHEVLFGVPDSVSAVNIRPANGAT
                     RVETTAILEWSAPLSYTPTGYDVYFGTNPDAAANPKVLDNALQTTYDPDLAYSTTYYW
                     QVDAYEGTIKHPGRIWSFTTRPEHIIKVACVGDSITYGYGIPDIGTNSYPAQLQALLG
                     AYYQVGNFGRSGARVSQISSNPYKGSPEYYAAMAFEPDIVFIALGINDCSVNQWQTNK
                     PVFAAHYKDLISDFKNLSSHPQIYIGTLMPVFCPPYPYPAIYSNMAECSPLIEQVAAE
                     EKLPLIDLYTPLESHSAYYAVDGLHPDRNGAQVIAQTVYRAIKPDVYISHCLLSVKEE
                     GATSDAYTIVLGGIPKQDVAVTLTPSDPEQVLIKVNGMQKDTIVFTPANCSQPQTISL
                     TAVDDRVFEGTHTVVISHICSSDDWAYDNLVIPDVSVNIADNDYYGKRVKVFLIGGQS
                     NAAGSGLNTEYPALYQTPQNDVEFWVGGRLPGDGVPDYSVWDRPADTAFRPLQLGSGN
                     YPAGTHSGFEMSLGRALKDALPYDNIALVKYGMSGSALMRGLRSSDGAGDWDPGPPNW
                     SPSEGFDGVRYHIFRTSGVLPALQAIVNRGDLPEIAGMFWMQGETDAGSSAAASQYEA
                     NLKKFIEAVRTDFGAPEMRFFIGRIRTVTGIYNAAVRTAMQEVAASDPLVMWIDTDDL
                     PVNADNVHYSAAGLVELGRRFADAYLRMASIQGDLEPDGAINLADFSRFAEQWLSVNC
                     GFCFGADLNSDLDVNMDDLTLLTDQWLDGSVSRCAARWAMDENSGNTAIDKGPNRYDG
                     TLINFPSDNTQWMKGIFGNALHFDGIDDYVVVPGYKGISGTASRTCSVWIKTIKPG"
BASE COUNT         1305 a         1381 c         1465 g         1349 t
ORIGIN      
        1 gttaccgtta cagctattga tgacaatttt tcggaaggcg tacataccgg tctgattact
       61 catcgatcca gttccgtact ggctgattat gatggtttaa atctgccgtc tgtggaattt
      121 gagattgcgg ataacgattt acctgtgatg ttcgtttcaa atgtttttgt cggcggtcag
      181 gaaggacctc aaggcacttc taattatcgg attcccagca tgactgtggc gccggacggc
      241 tctattctgg cctttgctga aggccgccgc aacagtgctg atccgggtgc tgtacttcca
      301 attgatatgg tgatgaagcg atctgtcgat aacggtcaaa catggcaacc gctggtggtg
      361 ctgcatcata acagctcatt tgattactcc gacccgcgtc cggtgacaga tcttctgaca
      421 ggaaagatac atcttcttta tacccaatgg cccaatttgt gcgggcaatc ctgcgtaccg
      481 gccggtttgg gcgataattc ttcagtggtg ttcctgcaaa cttccgaaga caacggtttg
      541 acctggtcgg gaccaatcaa cctgaacaca caggtcaaga atccgacgtg gaaggcactt
      601 aattctggac cgggccacgg catccagctg cgatggcaga ccaatccgac ccgaaacgga
      661 cgtctgctgg ctccctgcca tatcaacggc gttgatgccg tatcggtata cagcgatgac
      721 ggcggtctga gctggcaatc gggtacagtg gatacgacag cacccagttt gaatgagtcc
      781 gatgtagtgg aattgaccaa cggcgacctg ttgtgggatg cccgtcaagg cagcggatta
      841 taccgctatc gtcttaaaag caccgacgga ggccagactt ggcagtatct gggactcggc
      901 gatatttata ttacaccagt ggattgcggt atcgaccggt actccgccag acgggacggt
      961 catgaccgtg accggattgt cttcagcggg cctctgggca gtcccatcgg gacacccggc
     1021 cgctacaatc tggccgtctg gaccagctat gacgaaggca aaacttttat caatccgatt
     1081 cagattaata atggctttgc tgcttattca gacataaagc ggctggccaa tggccgcatc
     1141 ggtgttatct atgaggaaac cggcagcacg cttatccgtt ttgcaagctg taacatcgat
     1201 gtctttgaaa atgggcctca tgatccggct ttgacgcagt atgacggatt tggaaatgcc
     1261 gtcgatatcc gccgaggggg cgtgggctgg acaggctcat ggaccggatt gggctcattc
     1321 acccgctccg cctgtgccgc ctttcggggc tccagtattc cttttgaacg gtatcggttt
     1381 acgaatcagc ccggccgcgt cgattctgtc gccggcagcg ggatgcatat aacgcgtctg
     1441 ctgaacagcc catttgatat gcatgacaac ggcactatct acctgtcttt gctggtcagc
     1501 cgggcgctgg atacgaccgc caataacgat tctgaggaag atttgaacat tatgctgcaa
     1561 gacagttccc agcagcctta tatctctttt ggcgttaaca gccgggaagc gttttatgtg
     1621 ggcaaacccg gcagtgtgat tacgacagca gataatgccg ttgcgatgga ttgttcttat
     1681 ctgatgattg ccaaaattat cacaaaaccg gatcaggatg acaggattta tctgaaagta
     1741 cttcgttccg gcgccgatgc tgttgccggg caggaaatgg acatcgactg gacgctcatc
     1801 ggtgcatcgg gggagaatgc agccgcggtt ttggaccgga tctgcataac tgccggtgct
     1861 aatgcggtct ggtccgtgga tgaggtgcgc atcggtacaa cctattattc tgttgccggc
     1921 aggccggact gttctcagtc ggaaagctat ctgccgggcg atatcaaccg cgactgctat
     1981 gtcaatctga atgatcttgt attgctggtc aatgactggc tttcaccggg ctgttcggaa
     2041 caggggattt atccgcagat cgatattaac ggggattgtg atgtcaacat ggatgatatg
     2101 gctgggctgg ctgccggctg gctggattgc accgatccct ataatccaac ggaatgccgc
     2161 agtgtaaatt gatttcagat tattccggag cagaactctc actataacca gactaaacaa
     2221 ccgaatgcag caatgaaagc aatgaccatt tacaagtcta caagggtatt gggggagcat
     2281 tctgaatggg acaaccagga tgacagcatg acatttcaat tgattgtacg gatcctgctg
     2341 ttattaatcc cttcagcttg cggatataca tccaatatcg catcgtacaa ctacaccagc
     2401 agtctggggg ctgatccgac gacccaaggg tggacatact tgaccaatgt taccaacaat
     2461 gtgtatctaa gcggttatga tgcctctatc taccccggtc agagcggctg gcggatcgtt
     2521 gacggaacaa gcaccggcta tgccaattat cagattactc ttaccgacga ccagactgaa
     2581 gccatgaaga ccggtttctc tgtctcatgg accttcaagc tggatgcaac ggcttggaag
     2641 aacgacggca ccggcagtgt gacaaactac tacctgccgc cctatcaaag ccgccagaac
     2701 aacaacggcc tgtggattga gacggccgga gagaattcct tccgctacta cattgttgcc
     2761 acagccgatg caagcggcaa tctggcgatt aacgacggga cggtgaccca tgtgctgacc
     2821 gcaggcggcg gcaataacgg gtatgatacc ctgtatacgg taaccgtagc ctatgatggt
     2881 accaaggcag tgctctccta cggcggcagt gattttacac tgaccaagca ggcgactttc
     2941 ggcaataatc gggtgctgtt cggtgctcat accagccccg gacaaggcag cgtggtgtat
     3001 catgaggttc tgttcggcgt gccagattct gtgtcggcgg tcaacattcg gccggccaat
     3061 ggggccacca gagtagagac tactgctatt ctggagtggt cagcaccttt gtcttatact
     3121 ccgaccggct acgatgtgta ttttggcacc aatcccgatg ccgctgccaa tccgaaggtt
     3181 ttggataatg cccttcagac aacctatgat ccggatcttg cctactcaac aacctattat
     3241 tggcaagtcg acgcgtatga gggtacaatt aagcaccccg gccgaatatg gtccttcacc
     3301 acacggccgg aacatattat aaaagtggcc tgtgtcggcg acagtattac ttacggatac
     3361 ggcattccag atatcggaac caactcgtat ccggctcagc ttcaggcgct gttgggggct
     3421 tattatcagg tgggcaattt cggccgcagc ggagcaagag tttcacaaat ctcatcgaat
     3481 ccgtacaaag gcagtcccga atattatgca gcgatggcgt ttgaaccgga tatcgttttc
     3541 atcgctttgg gcattaacga ctgctcagtc aaccagtggc agacaaacaa accggttttt
     3601 gctgcgcatt acaaagattt gatcagcgat ttcaaaaatc tgtcaagtca tccgcagatt
     3661 tatatcggca ctctgatgcc ggtcttttgc cctccttacc cctatcctgc catttacagc
     3721 aatatggctg aatgctctcc tctgattgaa caggttgccg cagaggaaaa actgcccctg
     3781 atcgatctgt atacgcctct ggagtctcat tcggcgtatt atgctgttga cggactgcat
     3841 ccagaccgta acggggctca ggttattgcc caaaccgtct atagagcaat aaaacctgat
     3901 gtatatatca gccactgtct tctgtcggtt aaggaagaag gcgcgacttc agatgcttac
     3961 acaatcgtct tgggcggcat acccaaacag gatgttgctg tcacattgac tccatcggat
     4021 ccggaacagg ttttgattaa agtaaacggt atgcaaaaag atacaatcgt atttaccccg
     4081 gccaattgca gccagccaca aaccatctcg ctgactgccg tggatgatag ggtttttgaa
     4141 gggacgcata ctgtcgttat tagccacatc tgttccagcg acgattgggc ttatgataac
     4201 cttgtgatac cagatgtcag cgttaacatc gccgacaacg attattacgg caagcgggtt
     4261 aaagtgttcc tgataggcgg ccagtccaac gcggccggtt ccggcttgaa taccgaatat
     4321 ccggcccttt atcagactcc gcaaaacgat gtggaattct gggttggcgg ccgtttaccc
     4381 ggcgacggtg tccccgatta cagcgtttgg gatcgtcctg ccgatacagc cttccgcccc
     4441 ctgcagctgg gatcaggtaa ttatccggcc ggaacgcatt ccggttttga aatgtcgctt
     4501 ggccgagcat tgaaagatgc actcccctat gacaatattg cgctggtcaa atacggcatg
     4561 agcggttcgg ctctcatgcg gggacttcgc agcagtgacg gagccgggga ttgggatcca
     4621 ggtccgccaa actggagccc aagtgaaggt tttgacggcg ttcgctatca catatttaga
     4681 acatcgggag tacttccggc tttgcaggca attgtcaatc ggggggattt gcctgagatc
     4741 gcggggatgt tctggatgca gggagaaaca gacgccggca gttcagcagc ggccagtcaa
     4801 tatgaagcaa atctgaaaaa attcattgag gcggtccgga cagatttcgg tgcacctgaa
     4861 atgcggtttt ttattggccg cattcggaca gtaacgggaa tctacaacgc tgcagttcga
     4921 acagcaatgc aggaagtagc cgcttcggac ccattggtca tgtggatcga caccgacgac
     4981 ttgcctgtaa atgctgacaa tgtgcattac agtgcagccg ggctggtcga gctgggcagg
     5041 cgttttgccg atgcatacct taggatggca tctatacaag gggatcttga acccgacggg
     5101 gctatcaatc tggccgattt ttcccggttc gccgaacaat ggctctctgt taattgcgga
     5161 ttctgtttcg gtgcagactt aaacagcgat cttgatgtca acatggacga tttgactctt
     5221 cttacagatc aatggcttga tggttcggtc tcccgctgtg ccgctcgatg ggcaatggac
     5281 gaaaacagcg gcaatacggc tatcgacaag gggcctaatc ggtatgacgg cacattgatc
     5341 aacttcccat cagataacac gcagtggatg aagggcatat tcggcaatgc tttgcatttc
     5401 gacgggattg atgattatgt tgtggtgccc ggctacaagg gcatcagcgg caccgcttcc
     5461 cgcacctgca gcgtctggat caagacgatc aagcccggct
//
LOCUS       DAOTBO010000146         5492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12463_length_5492_cov_4.095825, whole genome shotgun sequence.
ACCESSION   DAOTBO010000146 DAOTBO010000000
VERSION     DAOTBO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12463_length_5492_cov_4.095825"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLE88_11955"
     CDS             <1..477
                     /locus_tag="PLE88_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77220.1"
                     /translation="QTGRFLICAEPIKNGAIGRAWADGIVTTRIDVLDPSHNYATVKP
                     EDATQLVSADQGLCYILYKETGTGTKWGVVRYGSTGNSLRWAFCSEDAGSGNTLDCFL
                     DTDGTGTLVTVYFKLLNCSSLDSGHLTLVDGVPIPVMKRGGNWWCIIPIEGTEVVV"
     gene            477..1793
                     /locus_tag="PLE88_11960"
     CDS             477..1793
                     /locus_tag="PLE88_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77221.1"
                     /translation="MVDQQPLCRNIDTDRIYRVNTGTNKLLRCLSPEFNTINLGCFLP
                     QLPIWTEGSTYAVGDIVFGRVEFGTPVVAYICGIFECRVAHTATTGNAPSASSGFGTC
                     QVQTTLHWRALGTTTLLEYPVCGSLASGGAEPYGGINKSPTGVLVELSGITPKPDHLD
                     YPEIAWHHDYNGVYCLPAMYGYTGGTWYIGGLCDGDLKWTLEFDPTLPWGDCETGTRK
                     MSIRQLKRTLTDNWWTWTYLFCGSHEVSLCETSYTDSNLFLPPPYYPYYWWDTDPLIV
                     AYRQSLIPARDCTGYDNYGAIPPFSGWGGTVSFLGFTDYMTVGFDDFNVEYFNDGESA
                     NVRWRFALFGSGGKRVILKNNIVCDFFRLDFQGSDWDIFLGCTTTPVTPDEDGWFETT
                     VSLPDNMTISDFGVTNVNAYNWKYRCHLSSDPAPGTSYLGSLRQSS"
     gene            1793..2194
                     /locus_tag="PLE88_11965"
     CDS             1793..2194
                     /locus_tag="PLE88_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77222.1"
                     /translation="MMGCCGKISKGFSIAKGNLAVVLEKINLLPADQFMYHSVRLRAC
                     RDCENHTYLTEQQYLDWIDTHGGLVKFVAEIDTLDQWPPLPIQQEQPGAKLFCSLCKC
                     WLPAKAYVKQENCPVQHPDWRKPKCFFKESS"
     gene            2194..3888
                     /locus_tag="PLE88_11970"
     CDS             2194..3888
                     /locus_tag="PLE88_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77223.1"
                     /translation="MAEKYWYGGVTNHKGEWDFEGISPFNLDAAGRATDAGTGLSGLP
                     ATAHGLMAGDSITIAGTDHFDGTHILNAATTNDILVIPVAYTVLSGSNSIVAGTQWTA
                     AGINLYTSGVQAGMRIKLWNGSTLLGAKYSGTNGIVSGTAFTASGVDFYAMGVEVGMT
                     VKLTGIGEFAITSITDATHLVIEDTGTSEAGLTWEIQVRDEATWGGFGIISVLDAGHL
                     QLESTGLSQSGVTYAIIRGETFDGTETVTPNIDSNWKLVADGSNASKPADTDVVHFND
                     RAYYDNTAQRYQGCTINVDSDGTGTPNLDGVFVSAGFNGNIGSEGQPLEINCDSEDIV
                     VDGECTIHLKLSAGVGNDAGCGRLVVNNEKANVYVSSLLNDATNVCLYDLLICLKGYL
                     WIQDNCAVGEVVSANYSSFIDAGVEIENIKDGNLCSITAVKGEIVWRSKIDQVKLFDA
                     EFSWGDEDMTEVVNYACNLLTIYSKSGEFQWQMADTGESLIKQFVLYAGNLIASQSVN
                     SGYKKKIGTGTEISELWPDATANLNSSSNNVLIESGSKLESHGGTLIASSGSLLGW"
     gene            complement(3927..>5492)
                     /locus_tag="PLE88_11975"
     CDS             complement(3927..>5492)
                     /locus_tag="PLE88_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOM77224.1"
                     /translation="AGEYSDDTQLILCICRSLGYGSNWYDFWTQTELPLWSLYERGGG
                     GATKRAAETWLKGIEPWDTIRNKNDVKRYFNAGGNGVAMRILPHVLLDGTTQDYDTIA
                     KNIFLDGITTHGHPRALVGALAFGYSLWSSLKRESKLEYGEIIDDLIKNIDSWSNIPN
                     LPSEFSKWQKAANEASKDYASQWKATLQEMMLLLNVCNEELSKGSLVIDEEALKKLNC
                     FNKDISGAGTVSAAGALYIASRYAPDPFHGLVKAANAQGADTDTIASMAGSLLGIIHG
                     TSWLSSLKNIIQDGKYLLKSVDDLIVKNAGESNAFKIPHLVSSKLKKWNDQLFKSQES
                     SNVEMPDGRKGKVHLLPDQVGKTGRYKIEFRKIILEDGQTIFMKKVTKGDFRVEKKNQ
                     DSTKIDGQTRHSCVHCGIKIPASSIAESSYFYKDILGFKVKKQSQEVVAFEQGVTLVP
                     MTYNNNFDPQFKSFSILFIEVENINEKYALIKKKGINILTPLSSWGKSLQARQFFRCA
                     DPDGNVIELFSVQ"
BASE COUNT         1403 a         1229 c         1340 g         1520 t
ORIGIN      
        1 caaaccggcc gttttttaat ctgcgccgaa cccatcaaaa atggtgcgat tggtcgggcc
       61 tgggcggatg ggattgtcac cactcgaatt gatgttctgg acccatccca caattatgcc
      121 accgttaagc cggaagatgc aacccagttg gtcagtgccg accagggact atgctatatc
      181 ctctataaag aaacgggcac cggcacgaaa tggggtgttg ttcgctatgg ttcgacgggc
      241 aattcgctgc ggtgggcatt ctgcagtgaa gatgctgggt ccggcaatac actggactgc
      301 ttcctggata cggatggaac cggcacactg gtaacggttt attttaagct gctgaattgt
      361 tccagtctgg acagtggcca tttgacgcta gttgatggcg tacccatccc cgtcatgaaa
      421 cgcggtggca actggtggtg cattatcccg attgaaggaa cggaggtagt tgtctaatgg
      481 tcgatcaaca gccgctttgc cgaaatattg acaccgaccg aatttaccgc gttaacactg
      541 gaaccaataa gttgctgcgt tgcctgagtc cggagtttaa tacgattaat ttaggatgct
      601 ttttgccgca actccccatc tggaccgagg gaagcaccta tgcggtcggg gatattgtgt
      661 tcggcagggt tgagtttggt actcctgtcg tcgcttatat ctgcggcatc ttcgagtgcc
      721 gggttgcgca caccgcaaca acgggtaatg caccctctgc cagtagtggc tttggaacgt
      781 gtcaggtcca aacaacgctt cattggcggg cattgggcac cacgacactc ttagagtatc
      841 ctgtctgtgg ttcactggcc tccggcggcg cggagccata tggtggcatt aataaatcgc
      901 caaccggtgt gttggttgaa ctgagcggta tcacccccaa gccggaccac ctggactatc
      961 cggagatcgc atggcatcac gattataatg gagtttattg cctcccagcc atgtatgggt
     1021 acaccggtgg aacatggtat atcggcggtc tctgtgatgg ggatctcaag tggacgctgg
     1081 aatttgaccc gacgctgcct tggggagact gcgaaacagg tacacgaaaa atgtccattc
     1141 gccagctaaa acgaacgctg acggataact ggtggacttg gacctattta ttttgcggtt
     1201 cgcatgaagt ttctctgtgc gaaaccagtt acactgacag caatctgttc ctgccgcctc
     1261 cgtactatcc gtattattgg tgggatacgg acccgctaat cgtcgcatat cgtcagtcgt
     1321 taattcctgc tcgagactgt acgggctatg ataactatgg tgcaattcct ccgttttccg
     1381 gctggggtgg gacagtaagt ttcctcgggt ttacggatta tatgacggtc ggatttgacg
     1441 atttcaacgt tgaatatttc aatgatggcg aaagtgccaa tgttcgatgg cgatttgccc
     1501 tgtttggctc cggcggcaaa cgtgtcatcc tgaagaacaa tattgtctgt gatttctttc
     1561 ggttggattt tcagggcagt gactgggaca tcttcttggg ctgtacaacg actcctgtta
     1621 ctcctgacga ggacggatgg tttgagacaa cggtttcatt gccggataac atgaccatct
     1681 ccgattttgg agttaccaat gtcaatgctt ataactggaa atatcgctgc caccttagca
     1741 gtgatccggc accgggaaca agctatcttg ggtcattgag acagagttca taatgatggg
     1801 atgctgtgga aaaatatcaa aggggttttc gattgccaaa ggcaatctgg cggttgtact
     1861 ggaaaaaatc aacctgcttc cggccgacca gtttatgtat cactcggtcc ggctgagggc
     1921 gtgtcgggac tgtgagaatc acacctatct gactgagcag cagtatctgg actggattga
     1981 tacgcatggc ggattggtca aattcgttgc cgagattgat acactggatc agtggccgcc
     2041 attgcccatt caacaagaac agccgggagc aaaattgttc tgcagtctct gtaaatgctg
     2101 gctgccagcg aaggcgtatg tcaaacaaga aaactgcccg gtccagcatc cagactggcg
     2161 aaaaccaaaa tgctttttta aggaatcctc ataatggctg aaaaatactg gtatggcggc
     2221 gtaacgaatc ataaaggtga gtgggatttt gagggaattt cgccatttaa tctggatgca
     2281 gcaggtagag cgacggatgc cggaacaggg ctaagcggac tgcctgcaac ggcacatgga
     2341 ttgatggcag gcgattcgat taccatcgca ggcacggacc atttcgatgg tactcatatc
     2401 ttaaatgccg caacgacgaa cgatatactt gttatcccgg ttgcctacac tgttctgtcg
     2461 ggtagcaact ctattgttgc cggtactcaa tggactgcag cagggataaa tctatacact
     2521 tccggcgtcc aggccgggat gaggataaag ctatggaatg gcagcacgct gctgggtgcc
     2581 aagtattcag gtactaacgg tatcgtatcg ggcactgcgt tcactgcgtc gggcgtcgat
     2641 ttctatgcaa tgggtgttga agtcggaatg acagtcaagc tgacaggcat tggtgagttt
     2701 gcgattacca gcattacgga tgccactcac ttggtgattg aagacactgg cacgtccgaa
     2761 gctggcctga cctgggaaat ccaagtgcgt gacgaggcaa catggggcgg ctttggaatc
     2821 atcagcgtcc tagatgcagg ccacttgcag ctcgaatcga caggtctttc ccaaagcggt
     2881 gtgacttatg caatcatccg gggtgaaacc ttcgatggca ccgaaaccgt tactcctaat
     2941 atcgattcca actggaaact ggtcgctgac ggaagcaatg ccagcaaacc agccgatacc
     3001 gatgtggttc attttaacga ccgggcctat tatgacaaca cggcccagcg ataccagggc
     3061 tgtacgatta atgtcgattc agatggcacg ggaactccga atctggatgg ggtatttgta
     3121 tcggccggat ttaatggcaa cattggcagc gaaggccagc cattggaaat caactgcgac
     3181 agtgaagata ttgttgtgga cggcgaatgt acaattcatt tgaaactttc tgctggagta
     3241 ggaaatgatg cgggttgcgg gcgattggtc gtcaataatg aaaaggccaa tgtctatgtt
     3301 tccagcctgc tgaacgatgc cacgaatgtg tgtttatatg acttgctgat ttgcctgaag
     3361 gggtatttgt ggattcagga caactgcgca gtcggcgagg tggtatctgc aaattatagt
     3421 tcatttattg atgcgggtgt tgagattgaa aatatcaaag atggaaacct atgttctatc
     3481 acagccgtca agggcgagat cgtttggcgg tccaagattg accaagtcaa acttttcgac
     3541 gctgagtttt cttggggtga cgaagatatg accgaagtgg ttaattatgc ctgtaatctg
     3601 ctgaccatct actccaagag cggtgagttc caatggcaga tggcggatac aggtgaatcg
     3661 ttgattaagc aattcgtgct ttatgccgga aacctgattg cctcccagtc ggtcaacagc
     3721 ggctacaaaa agaaaatcgg caccggcacc gaaatcagcg agctatggcc ggatgcgaca
     3781 gcgaacttga acagttcgtc caacaatgtg ctcattgagt cgggttccaa gctcgaatcg
     3841 cacggaggaa cgctgattgc atccagcgga tcgttgttag ggtggtaaat aactaagagg
     3901 aaagcgggct taactcatat tataattcat tgaacgctaa ataattcaat aacattccca
     3961 tcaggatcgg cgcatcgaaa gaactgtcgt gcctgcaagg actttcccca tgaacttaag
     4021 ggtgttaaga tattaattcc ttttttctta ataagagcgt acttttcatt gatgttttca
     4081 acttcaataa atagtattga aaaagactta aattgggggt caaagttatt gttgtaagtc
     4141 ataggcacaa gagtgactcc ctgctcaaaa gctactactt cttgagattg ctttttgact
     4201 ttaaatccta agatatcttt ataaaaataa gatgactctg caatagaact ggcggggatt
     4261 ttaatgccgc agtgtacgca tgaatgtcga gtctgtccgt ctatcttagt tgaatcttga
     4321 tttttctttt ctacacggaa atccccttta gttaccttct tcatgaatat agtttgtcca
     4381 tcttcgagta taatttttcg gaactctatt ttataacgcc cagttttccc tacttgatcg
     4441 ggtaaaaggt gaaccttacc ttttctacca tctggcattt caacgttaga actttcctgc
     4501 gatttaaaca actggtcatt ccacttttta agtttacttg acactaaatg gggtattttg
     4561 aatgcattgc tctcaccagc gttttttaca ataaggtcgt ctacgctttt gagtaggtac
     4621 ttgccatctt gtattatatt tttcaatgaa gacaaccatg atgttccatg tataattcca
     4681 agaaggcttc cggccattga agcaattgta tcagtgtccg cgccctgcgc atttgccgct
     4741 ttgactagac catggaacgg atctggagca tatcttgaag caatatatag ggcaccagcg
     4801 gctgaaaccg tgcctgcccc acttatatct ttattgaaac aatttagttt ctttaaagct
     4861 tcttcatcaa tgaccaaaga tcctttactt aactcctcat tgcatacatt caatagcaac
     4921 atcatctctt gaagagtcgc tttccactga gaagcataat ctttcgatgc ttcatttgca
     4981 gctttttgcc acttgctaaa ttctgatggt agattgggga tattggacca gctatcaata
     5041 ttttttatta gatcatcaat aatctcacca tattccagct tggattcacg ctttaaagaa
     5101 ctccaaaggg aatatccaaa agccaatgct ccaactaatg ctctgggatg accgtgtgtt
     5161 gtaataccat ctaaaaaaat gttttttgct attgtatcat aatcttgagt agttccgtct
     5221 aaaagcacat gtgggagaat acgcatagca acgccattcc caccagcatt gaaatatctt
     5281 tttacatcat ttttattcct aatcgtatcc cacggctcaa ttcctttgag ccacgtttct
     5341 gctgcacgtt tagttgctcc acctccacct ctttcatata aactccaaag aggcagttct
     5401 gtttgtgtcc aaaaatcata ccaattcgag ccatatccca agcttcgaca tatacacaat
     5461 attaattggg tatcgtcgct atattctcca gc
//
LOCUS       DAOTBO010000147         5452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12578_length_5452_cov_4.856958, whole genome shotgun sequence.
ACCESSION   DAOTBO010000147 DAOTBO010000000
VERSION     DAOTBO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12578_length_5452_cov_4.856958"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /gene="ltrA"
                     /locus_tag="PLE88_11980"
     CDS             <1..1682
                     /gene="ltrA"
                     /locus_tag="PLE88_11980"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOM77225.1"
                     /translation="GFSMNVEEVQRRLWEQSNGHKQYRQSGTPLFAVNPYEKRIRNLM
                     DLMHQPDWLKEACERVMVRSRGKAPGVDGVTTRMFQKGLEGKLEMLRLELKHGTYLPQ
                     PVKRVMIPKPNGKMRALGIPCLRDKIVQEAMRMALEPIFEVEFHDNSYGFRPNRNTHH
                     AVFRCQQLMQRNFTWVIEGDVKACFDEISHKSILKALREKVMDNRFLDLVCRFLKAGV
                     EIEGVVQPTEKGVPQGGVISPLLANVVLNKLDWFLQHKGTHGNVMQRAARKREPNVRF
                     ARYADDWCVFITRASKRYAETLRDQIQQFLKHECELELSMEKTHVTHVRDGFEFLGFR
                     LTLEIGQSGRPVPKIKISLDAKRNLKQRLEEAIRRRPQQESIALRINRGSAVVRGWSE
                     YYRIACDFTDKAATFDHWAFWTAIKAICRKLDIPTGACMKRFYRNTTVYVDEYCQLET
                     FSGKKMKLDYRGPEPYMPGNGTYETDPQMDADFSKYAERQRFGSNDHKYLALKHDGYK
                     CQKCGKPVTDLTSQVDHIVPVKCFASFALASTPENLQTLCLDCHEEKHHAE"
     gene            1708..2541
                     /locus_tag="PLE88_11985"
     CDS             1708..2541
                     /locus_tag="PLE88_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOM77226.1"
                     /translation="MLRKLHVRFGVGVGVQSPDLHHVKVEPRSNNAIAAGLSSFGKGL
                     QHHQSFDVARDKTKAQKTTPKLRAKDRPLENDFVSKEDFDEMLLAWFNNASRVLRPGG
                     SFYIWGGYANLGNYPGPLKAAGLYFSQGIVWDKQHPVLTRKDYMGAFEICFYGWKEGA
                     GHKFYGPNNATDLWHVKKVNPQSMIHLTEKPVELAVRSIQYSSKPGGNVLDLFGGSGS
                     TLIGCEQTDRKAFLMEIDPLYCDVIVNRWEQFTGKKAERIVATEKTSAQMTEVSEEVT
                     V"
     gene            complement(2550..3074)
                     /locus_tag="PLE88_11990"
     CDS             complement(2550..3074)
                     /locus_tag="PLE88_11990"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOM77227.1"
                     /translation="MKKSDVKIGNHYKAKVTDKIVTVEILAENPHGGWDAKNLSTGKT
                     VRIKSPQRLRGAAPKPNTVAPVGKPKEKPSKDTPKAKSPTTPKPVQNTLRTKKMSILD
                     AAAQVLSEAKEPLNCKLMIKAMTDKAYWEPAQSGKTPANTLHAAISKEIKTKAGESRF
                     EKVGRGQFALSPQK"
     gene            complement(3099..3368)
                     /locus_tag="PLE88_11995"
     CDS             complement(3099..3368)
                     /locus_tag="PLE88_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77228.1"
                     /translation="MHIIEMKLAGHMNKGDVPEYFATIHRVPGNDYITVKLISANGER
                     EHLVEANNPDDVYSMAECLQYHLDGCKGTNSMVHDYYRILENFTD"
     gene            complement(3387..3539)
                     /locus_tag="PLE88_12000"
     CDS             complement(3387..3539)
                     /locus_tag="PLE88_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77229.1"
                     /translation="MLNAKVTITKGPHKGKPGRIIGVYVQDKKYDVRINRQYYAIPFK
                     YVKTNA"
     gene            complement(3558..3995)
                     /locus_tag="PLE88_12005"
     CDS             complement(3558..3995)
                     /locus_tag="PLE88_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77230.1"
                     /translation="MNCERSIKRDLIKKIQLLRIDRPLVSTRAGETMNMDEVIESTNG
                     YQKKPLAELQWLLLNERAVREINKMFPPVHPHHPAAFIRLDETQAMISGESMVYEHIN
                     GETFETTLNDYYENYIHPKLTEWAEKNHCHFEWENPAGICLAL"
     gene            complement(4011..4253)
                     /locus_tag="PLE88_12010"
     CDS             complement(4011..4253)
                     /locus_tag="PLE88_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77231.1"
                     /translation="MEIELNNNDEKMKNAYNEAKTDIVNLLGWFECEMQKQPAKLNWG
                     HVGSLNHIRENLIETLAFMSGFDAKEIKNTLEETKL"
     gene            4395..4595
                     /locus_tag="PLE88_12015"
     CDS             4395..4595
                     /locus_tag="PLE88_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77232.1"
                     /translation="MGKTIKITALTADELATLLSQASRKAISGQEVLAIAEMAGIVAP
                     DGTINLIDYTAFLAQEVAGGAD"
     gene            4582..>5452
                     /locus_tag="PLE88_12020"
     CDS             4582..>5452
                     /locus_tag="PLE88_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77233.1"
                     /translation="MAQINPTKLKPTDLTRLLNSAGFGEVLNERTLRRHRNRAGYTIG
                     DARTINLFRYAAWLTQQYLAPPKDGRSYDEIREAARLRNAELARAGQDIGQIPAVVNP
                     DRKAKAMASFKYFCEVYFPEVFYLTWSADHHKVITKIEQAVLKGGLFALAMPRGSGKT
                     VLMQMACLWSALIGATEFVCLIAASADRAKNLLENIKVWLETNQLLQEDFPEVTLPIL
                     ALERITNRQKGQKHNGIPTRIEWAADKIVLPTIEGSKASGVVISCSGMKGSDIRGQNH
                     ARADGKVVRPQLVM"
BASE COUNT         1308 a         1265 c         1466 g         1413 t
ORIGIN      
        1 gagggttctc tatgaacgta gaagaagtcc aaagaagact ttgggagcaa tcgaacgggc
       61 acaagcagta ccgacaatca ggaactcctc tgttcgcggt taatccgtat gaaaagagga
      121 ttcggaatct gatggactta atgcatcagc cggactggtt gaaagaagcc tgtgaacgtg
      181 tcatggttcg ttcccgaggc aaagcaccag gcgtagatgg agtgacaacc cgaatgttcc
      241 agaagggact ggaaggtaaa ctcgaaatgc tacgccttga gttgaaacat gggacctatc
      301 tacctcagcc cgtcaaacgg gttatgatcc cgaagccaaa cggtaaaatg cgggctttgg
      361 gcattccctg tctgcgggat aaaatcgttc aggaagctat gcgtatggcc ctggaaccga
      421 tttttgaggt agaattccat gacaactcgt acgggtttcg gcccaatcga aacacgcatc
      481 acgccgtttt ccgttgtcaa caactcatgc aaagaaattt tacatgggtg attgaaggag
      541 acgtcaaggc gtgttttgat gaaatctcgc acaagtccat tctgaaggcc ttgcgggaaa
      601 aagtgatgga caacagattc ctcgatctgg tctgccgctt tctgaaggca ggcgtagaaa
      661 tcgagggggt tgttcagccg accgaaaagg gagtccctca aggcggcgta atctcacctt
      721 tattggcgaa tgtggtgctc aataaactgg attggttcct gcaacataag ggtacgcatg
      781 gcaatgtgat gcagcgtgca gcgcgaaagc gggagccaaa tgtccggttt gccagatacg
      841 ccgatgactg gtgtgtgttt atcacgcggg cgtcgaagcg atatgccgaa acgctgcggg
      901 atcaaatcca gcagtttctg aaacacgaat gtgaattaga actgtcgatg gaaaagacac
      961 atgttacgca cgttcgagac ggatttgaat ttctcggatt ccgcctaacc cttgagatcg
     1021 gacagagtgg gagaccggtg ccgaaaatta aaatcagcct ggatgcaaaa cgtaatctca
     1081 agcaacggct ggaagaagca attcgccgaa ggccccaaca ggaatccata gcgctgcgga
     1141 taaaccgtgg atcggctgtg gttcgcggat ggtcagaata ctaccgtatt gcctgtgact
     1201 tcacagacaa agcggcaacc tttgaccact gggccttctg gacggccatc aaagcgatct
     1261 gtcggaaact cgacattcca accggcgcat gtatgaagcg gttctatcgt aacacgacag
     1321 tctatgtgga tgagtattgt cagcttgaaa cattcagtgg caaaaagatg aagctggact
     1381 atcggggacc ggaaccttac atgcccggca atggaacgta tgagacagac ccacagatgg
     1441 atgctgattt cagcaagtat gccgaaagac aacgttttgg aagtaacgat cataagtatc
     1501 tggcgttgaa gcacgatgga tacaaatgcc aaaaatgcgg aaaacccgtt acggatctta
     1561 cttcacaggt tgaccatatt gttcccgtta aatgctttgc cagttttgca ttggcgagta
     1621 ctcctgaaaa tctacaaacg ctctgtttgg attgccatga ggaaaaacac catgcagaat
     1681 aatgtcaaga aatatctgga aagccggatg cttcgaaagt tgcacgtccg gtttggggtt
     1741 ggggtcgggg tgcaatcccc cgacctacac cacgtcaagg ttgagccgcg gagcaataat
     1801 gccattgcag ccggattgag cagctttggc aagggattgc agcatcacca gtcctttgat
     1861 gtggcccgtg ataaaaccaa ggctcagaaa accaccccaa aactgcgtgc caaggaccga
     1921 cccctggaga atgatttcgt ttcaaaagag gatttcgatg aaatgcttct cgcctggttt
     1981 aataatgcct cacgtgtatt acgtcccggc ggttcgtttt atatctgggg tggctatgcc
     2041 aacctcggga actatccggg gccgctgaaa gcagcgggcc tgtacttttc acaagggatt
     2101 gtgtgggata agcagcatcc ggtcctgacc cgcaaagact acatgggcgc ttttgagatt
     2161 tgcttctatg gctggaagga aggtgcagga cacaaattct acggaccgaa caatgccacc
     2221 gacctgtggc atgtcaaaaa ggtcaatccg cagagcatga tccatctgac ggaaaagccg
     2281 gtggaactgg cggtgcgttc aattcagtat tcctccaaac ccggcggcaa tgtcctggat
     2341 ttgttcggcg gcagcggcag cacgctgatc gggtgcgaac aaacagacag aaaagcattc
     2401 ctgatggaaa tcgacccgtt gtattgcgac gtaatcgtta accgttggga acagttcact
     2461 ggaaaaaagg ccgagcggat tgtggccact gagaaaacct cggcacaaat gaccgaggtt
     2521 tcagaagagg tgacggttta atgttgccgt tacttttggg ggctgagggc aaattgtcct
     2581 cggcccacct tttcaaagcg ggactcgcca gccttggtct tgatttcctt gctgatggcc
     2641 gcatgcagcg tattggcggg tgttttgccg gattgggcag gttcccagta ggctttatct
     2701 gtcatcgcct taatcatcaa tttgcaattg agcggttcct tcgcctcgga aagcacctgg
     2761 gcagccgcgt ccaggatgga catcttcttg gttcgcagtg tattttgcac aggtttgggt
     2821 gtggtgggcg atttggcctt gggggtatcc ttgctcggct tttccttcgg tttgccaacg
     2881 ggggcaaccg tgttgggttt gggggccgcc ccccgtaacc gctgcggcga cttgatccgc
     2941 accgttttcc cggtggataa gtttttggcg tcccaaccgc catgcggatt ttcggccagg
     3001 atttctacag tcacaatctt gtcggtaact ttggctttgt agtggtttcc aattttgaca
     3061 tctgattttt tcattgttcg ggtcctttca aaaaaatgtt agtctgtaaa gttttccaaa
     3121 atacggtaat agtcatgcac catcgagttg gtgcctttgc atccgtcgag gtggtattgc
     3181 aggcattcag ccatcgagta gacatcatca ggattgttcg cttcaaccag gtgttcccgt
     3241 tcgccgttgg cgctgatgag tttgacggta atgtaatcgt tgccggggac gcggtggatg
     3301 gtcgcgaagt attcaggcac gtcgcctttg ttcatgtgtc cggccagttt catttcgata
     3361 atatgcattg ttcgggtcct ttcggtttag gcattggtct taacgtactt aaatgggata
     3421 gcatagtact ggcggttgat acgaacgtca tactttttgt cctgcacata caccccgatg
     3481 atgcgtccgg gttttccttt atgcggtcct ttggtaatgg tgacttttgc gttcagcata
     3541 gcgttggtcc tttcgtatta aagagcaagg cagattcctg cggggttttc ccattcaaaa
     3601 tggcaatggt tcttttctgc ccattcagta agtttgggat ggatatagtt ttcataataa
     3661 tcgttgagcg tcgtttcaaa tgtctcgccg ttgatgtgtt cgtacaccat gctctcgccg
     3721 gaaatcatcg cctgcgtttc gtcaaggcga atgaaagccg ccgggtgatg tggatggacc
     3781 ggcgggaaca tcttgttgat ttctcgaacg gcccgctcgt tcagcagaag ccattgaagt
     3841 tcggccagcg gttttttctg gtatccattg gtggattcga tgacctcgtc catgttcatc
     3901 gtttcgccag cccgcgtcga aaccaagggg cggtcgattc tgagcaattg gattttcttg
     3961 ataaggtctc gttttatgga gcgttcgcag ttcatggttc gggtcctttc ttagagttta
     4021 gtttcttcga gtgtgttttt gatttccttg gcatcaaatc cagacatgaa cgccagcgtc
     4081 tcgatcagat tttcgcgtat gtggttcagg cttcctacat gaccccagtt cagtttcgcg
     4141 ggttgtttct gcatctcgca ttcgaaccag cccagcaggt tgacgatgtc ggttttggct
     4201 tcgttgtagg cgtttttcat tttttcgtcg ttgttgttga gttcgatttc cattcttaag
     4261 tcctttctgt ataaggtttt atcgttcatc tacatgacac attacaattt attcaaaact
     4321 gcatcaagct aattaagtgc ttattataaa agaatttaca ttaattttta cgttccggac
     4381 aaggggtgta agcaatggga aaaaccatca aaattactgc attaacggcc gatgaactgg
     4441 ccacattgtt aagccaggca agccgcaagg ctatatccgg acaagaagtg ctggcaattg
     4501 ccgaaatggc cgggatcgtg gcaccggatg gcaccatcaa cctgattgat tacaccgcat
     4561 ttttggcaca ggaggtcgcc ggtggcgcag attaatccga ccaaactcaa gccgactgac
     4621 ctgacgcggt tgctgaattc cgctggattt ggggaagttc tgaatgaacg caccctccgg
     4681 cgtcaccgca accgtgccgg atacaccatc ggtgatgcgc gaaccatcaa tctatttcgg
     4741 tacgccgcct ggttgacgca gcaatattta gcgccgccca aagacggacg cagttatgac
     4801 gagattcgag aggccgcccg attgcggaat gccgaattag cgcgcgccgg tcaagatatt
     4861 ggtcaaatcc ctgccgttgt taatccggac cgcaaagcca aggcgatggc cagcttcaaa
     4921 tatttttgtg aagtgtattt tccggaggtg ttctacctga cgtggtcagc agaccatcac
     4981 aaggttatca caaagattga acaggcagtt ttgaaaggcg gtctgtttgc gttggccatg
     5041 cctcgtggtt ccggtaaaac ggttttgatg cagatggcct gtttgtggtc ggcgttgatc
     5101 ggagctacgg aattcgtctg cctgattgct gcttctgccg accgcgccaa gaatcttctg
     5161 gaaaacatca aggtctggct ggaaaccaat caactgctgc aggaggattt tccggaggta
     5221 acacttccaa ttttggcatt ggagcggatt accaaccgcc aaaagggtca aaagcacaat
     5281 ggtatcccga cccgaattga atgggcggca gataagattg tactgcctac catcgaaggc
     5341 agcaaagcat ccggtgtggt catctcctgt tccggcatga agggttcgga cattcgcggt
     5401 caaaaccatg cccgtgccga tggcaaggtc gtccgtccac aattggtgat gg
//
LOCUS       DAOTBO010000148         5451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12582_length_5451_cov_5.404930, whole genome shotgun sequence.
ACCESSION   DAOTBO010000148 DAOTBO010000000
VERSION     DAOTBO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12582_length_5451_cov_5.404930"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2860)
                     /locus_tag="PLE88_12025"
     CDS             complement(<1..2860)
                     /locus_tag="PLE88_12025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOM77234.1"
                     /translation="MKTKKTFSEGAMKRYSILGLVLSSILLSFCYGAEYREGYLLVKF
                     TDTGTTAASNAARQAVVNAAGGGTIQRMYTLVPGLGLVKLPAGVDVLTAKAAFSASAG
                     VQYAEPDYIQKVQAVPNDPDFARLWGMHNTGQTGGTKDADIDAPEAWDLAKGSSSIVV
                     AVIDSGVDYTHPDLADNMWINLAEQNGQVGVDDDNNGYIDDVYGYDFANMDGDPMDDH
                     GHGTHCAGTIGARGNNNVGVAGVNWQVKIMALKFLTAGGGGSTADAISAVQYAVRMGA
                     KLTNNSWGGYGFSQALYDAIEAAGKSGQLFVAAAGNESNNNDGDTPAYPGSYDLDNIV
                     SVLATDDTDTMASFSNWGKTTVDLAAPGVAIYSTAPGGRYQNMSGTSMACPHVAGAAA
                     LIWSIKPDLTYSEVKQVLMLSVDIKNTLEGMCVTGGRLNLYNAVLLAQGGDNLPPLPD
                     PAQWLIKPTATGLSTIVMQAVTAKDKSGVEYRFDCIEDDAYDSGWQSSSLYMRSDYPQ
                     GATYSFRVQARDKSANRNQTQWSETLQTTTATGVDDLPPAPNPTIWLAYPRVTRPSLN
                     EIGMEVVASYDENGVEYYFDCVFASDGNQDNYDSGWQTSPVYTVRVSQVAGHEYTFAA
                     YVRQKDTTDPVYITEAAAPMTAGRAASSIVREVPSAAYRTIQDAINAANNGDTVLVHE
                     GVYREININFKGKAITVCSENPDNPAIVAATVIDCADPQNLWALERRRAFLFQNMEGR
                     NSVLAGFTIKNAIAIDNPETRFGGSRSGVSDRDLPSPNDGRDAMGGAIVMGSPLAPAS
                     PTIRNCVFTDCFAYGQYGEHGVNGPNGGVNQDGQNGDRGGNGGNAYGGAIYAYSGSAP
                     LIKGCQFINCQAVGGEGGNGGNGGDGGGHSDNVEEADGHHGGHGGDAGLGGCAWGGAM
                     YFESDCSPELYDVTVRDCFVRVGEAGRGDVPEMEGFVSD"
     gene            3255..4553
                     /gene="aroA"
                     /locus_tag="PLE88_12030"
     CDS             3255..4553
                     /gene="aroA"
                     /locus_tag="PLE88_12030"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOM77235.1"
                     /translation="MILTASASTLSGSIAIPGSKSHTIRAVVISSLAKGTSRIRKPLV
                     SSDTLSAVDCYRALGAEIDISDNDCWTVIGNGGIIPSESRKIDVGNSGTTMNFAIGTC
                     SMANPNVEMYLTGDHQIQYRPVKPVLKAVIDLGAKGYCLKNNGCPPVSVSGRLRGGKT
                     TIECKSSQYLSSLLLCCPLAEGNSEITVSLLYEPDYARMTLDWLNKQGICYEMKDDLS
                     WFRIPGGQAYRPFDLPVPADFSSATFFLCAGALLGQNVIIKGLDFSDSQPDKAVVDYL
                     KQMGADITICQDGVYVSKSDLQGIEIDMNRTPDALPAMAVTAAFAQGTTKLVNVAQAR
                     MKETDRIACMAKELTKLGADVRELPDGLIIHGQGGKNLKAGSFSGYGDHRIVMAMSIA
                     AMSLEGKSRIDTAEAMSVTFPEYVALMKKLGADLQVPLHD"
     gene            complement(4573..>5451)
                     /locus_tag="PLE88_12035"
     CDS             complement(4573..>5451)
                     /locus_tag="PLE88_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77236.1"
                     /translation="EHMQRYSDNPTVFYDDADTGLLAAAVPNAPQFGYFGYLKKMILT
                     LHNIVMMKRGRMPYHGAMVRISLKNGKSASIVIIGDTAAGKSETLEAFRLLGRDQICQ
                     MKIIADDMGSFDIDVEGRLIGYGTEIGAFIRLDDLQAGYAFEQLDRSIIMSPQKTNAR
                     VVLPVTTLNEVLHGYPVDILLYANNYEQVDQDHPAIEFFDSPSRAIEVFREGASMAKG
                     TTTSTGLGHNYYANIFGPPQYRAMHDKLAETVFVKAFEAGVRVGQMRSRLGIPGYEAS
                     GPKVAAEELFKIISSE"
BASE COUNT         1397 a         1496 c         1254 g         1304 t
ORIGIN      
        1 tgtcggacac gaagccttcc atctcgggga cgtcgccgcg gccggcctcg ccgacacgaa
       61 cgaagcagtc tctgactgta acatcataca attccggcga gcagtcagac tcaaaataca
      121 tagccccgcc ccaggcacat ccgcccaagc cggcatcgcc tccgtgaccg ccatgatggc
      181 catcggcttc ttccacatta tcggaatgtc cgccgccgtc gccgccgtta ccgccgttgc
      241 ccccttcccc gcctacggcc tgacaattga tgaactgaca tcctttaatc aggggagccg
      301 aaccgctgta ggcataaatc gcaccgccgt aagcattgcc gccgttgccg cctctgtcgc
      361 cgttttggcc gtcctgatta acgccgccgt ttggcccgtt aactccatgc tctccgtatt
      421 gtccataggc aaaacagtcg gtaaaaacgc agttgcggat ggtcggactg gcaggagcaa
      481 gcgggcttcc catcacaata gctccgccca tagcatcgcg gccgtcattg ggcgaaggca
      541 ggtctctgtc ggaaacacct gacctcgagc ccccaaaccg ggtctccgga ttgtcaatag
      601 caatagcatt ttttatcgta aatccggcca gtaccgaatt gcgcccttcc atattctgga
      661 acaaaaaggc ccgccggcgt tctaatgccc aaagattctg cggatcggcg cagtcaatca
      721 ccgtagccgc aacaatagcc ggattatccg gattttcgct gcagacggtt atggctttgc
      781 ccttgaaatt gatattgatc tcgcggtaaa caccttcatg gactaaaacc gtgtctccat
      841 tattggccgc gttaatagca tcctgtatcg tccgataggc cgccgagggc acttcgcgca
      901 caatcgaact ggccgccctg cccgctgtca tcggagcagc cgcctcggtt atatacaccg
      961 ggtctgttgt atccttttgg cgaacatacg ctgcaaaggt atattcgtgc ccagcgactt
     1021 gacttacgcg aaccgtatag accggcgaag tctgccagcc gctgtcatag ttgtcctgat
     1081 tgccgtcgga agcaaagaca cagtcaaagt aatattccac cccgttttca tcgtacgagg
     1141 caacaacttc catcccgatt tcgtttaagc tgggacgcgt gacacgaggg tatgccagcc
     1201 agatagtggg attgggagcc ggcggcaggt catcaacacc cgttgccgta gtagtctgga
     1261 gcgtttctga ccattgggtt tgattccggt tggccgactt atcgcgtgcc tgaacacgga
     1321 atgaataagt tgccccttgc ggataatccg aacgcatata gagagagctg ctttgccatc
     1381 cgctgtcgta ggcatcatct tcgatgcagt cgaagcggta ttccaccccg gatttatctt
     1441 ttgctgtaac cgcctgcatg acaatcgttg acagacctgt agccgtcggc ttaataagcc
     1501 attgggccgg atccggcagc gggggcagat tatcaccgcc ttgggccagc agcacagcat
     1561 tgtataagtt cagccggccg cctgtcacgc acatcccctc aagtgtattt ttaatatcca
     1621 ccgacagcat cagcacctgt ttaacctccg aataggtcaa gtcaggctta atggaccaga
     1681 tcagtgcggc agctcccgcc acatgaggac aagccattga tgttccgctc atattttgat
     1741 atcgtccgcc cggtgcagtg ctgtaaatag caacgcccgg tgcagcgagg tccacagttg
     1801 tcttgcccca gttggaaaat gatgccatcg tatcggtatc atctgtagcc agcaccgaaa
     1861 caatattatc taaatcataa ctgcccggat atgcaggcgt gtctccgtca ttattgtttg
     1921 actcattgcc cgcagccgct acaaacaact ggccgctttt gcctgcagct tcaattgcat
     1981 catataaggc ctgactgaag ccatagcccc cccaggaatt gttggtgagt ttggccccca
     2041 tccgcaccgc atactgaaca gcgctgattg catccgccgt actgccgccg ccgccagcag
     2101 tcaaaaactt caaggccata attttgacct gccaattgac gccggctaca cccacattat
     2161 tgtttccccg ggctccgatg gtgcctgcgc aatgagtgcc gtgcccatgg tcatccatcg
     2221 gatcaccatc catattggca aagtcatatc cgtaaacgtc atcaatgtag ccattgttgt
     2281 catcatcgac gccaacctgt ccgttctgtt cggccagatt gatccacata ttgtctgcta
     2341 aatccggatg cgtatagtca actccgctgt caatcacagc aacaacaatg ctgctgcttc
     2401 cttttgcaag gtcccaagcc tccggcgcat caatatcggc atctttcgtt ccgccggttt
     2461 gcccggtatt gtgcatcccc cacaaacgcg caaaatcagg atcattggga acggcttgaa
     2521 ccttctggat atagtcgggt tcagcatact gaacgcctgc tgaagccgaa aacgctgctt
     2581 tggctgtaag aacatccacc cccgccggca gcttaaccaa tcctaagccg ggaacaagcg
     2641 tgtacatccg ctggatggtg cctcctccgg cggcgtttac cacagcttga cgtgctgcat
     2701 tggatgcagc cgtagtccct gtatccgtaa acttgaccag cagataacct tcacgatatt
     2761 ccgccccata acagaatgac agaagaatcg aagataaaac caaccccaaa attgaatagc
     2821 gcttcatagc accctccgaa aaggtttttt tcgttttcat ctcaagcgtc ccgaattagc
     2881 aaagttaaaa cgaatacgac cggggcgcag taccgcaccc cagccccgat ccaatcccta
     2941 aacatatcgg aatttgtaac tggtgagttt agttaagata tatatgaaaa cccgcttata
     3001 gtcaagggaa aacatatttc cccatcttcc ccagaccccc aaaattgaat attatgagac
     3061 ccaaaaacat atatatagaa atatcaccgt tacgtccgat atacatttca gtttacacaa
     3121 tatgaaactc ttactgtcaa aaaaaattaa aaaaaatata ctttttttaa ttttttaatt
     3181 ataacccttc tgctgtactg taaaattaac gagcttaacc aatatatgtt atctggaata
     3241 tacagaataa atgtatgata ctaacagcat ccgcttccac tttatcgggc tcaatagcaa
     3301 tacccggctc taaatcgcac actatccgtg ccgtcgttat cagttctctg gccaaaggca
     3361 caagccgcat acgaaagccg ctggtttcca gcgacacact cagtgcggtg gattgctatc
     3421 gtgcattggg agctgaaatt gacatttcag acaacgactg ctggaccgtc atcggcaatg
     3481 gaggaataat cccgtctgaa agcagaaaaa ttgatgtcgg caattcagga acaacaatga
     3541 actttgccat tgggacgtgc tctatggcaa atccaaatgt tgaaatgtat ctgaccggcg
     3601 atcatcaaat acaatatcga cctgtaaaac ctgttttgaa ggccgttatc gatttggggg
     3661 ccaaaggata ctgtctaaaa aataacggct gcccccctgt gtcggtcagc ggcagacttc
     3721 gcggcggcaa aactaccata gaatgcaaat caagccaata cttgtccagt ttactgcttt
     3781 gctgtccatt agccgaaggc aattctgaaa tcactgtttc cctgctgtat gagccggatt
     3841 atgcccgaat gacgctggat tggctgaata agcagggtat atgctatgaa atgaaagacg
     3901 atttaagctg gtttcgcata cccggcgggc aggcctatag accgtttgac ctgccggtac
     3961 cggccgattt ttccagcgca actttcttct tatgtgcggg ggcactgctt gggcaaaatg
     4021 tgattattaa agggttggac ttcagcgaca gccagccgga caaggccgtt gtagactacc
     4081 taaagcagat gggagcagat ataactatct gccaagacgg agtttatgtg tcaaaatccg
     4141 acttgcaggg catagaaatt gatatgaatc gaacaccgga tgcccttcca gcaatggccg
     4201 ttacagccgc ctttgcgcag ggaaccacaa agcttgtcaa tgtagcacag gcccggatga
     4261 aagaaactga ccgtatcgcc tgtatggcaa aggagttaac taaactcggc gcagatgtcc
     4321 gcgagcttcc ggatggtctt atcatacacg gccaaggagg caaaaatctc aaggccggct
     4381 cgtttagcgg ctatggtgac caccgcatcg taatggctat gagtattgcg gctatgtcac
     4441 tggaaggcaa aagccgtatc gataccgctg aagcaatgag cgttacattc cccgaatatg
     4501 tcgctttgat gaagaagctc ggagccgatt tgcaggtacc cttacacgat tgagacaata
     4561 tccaaacgca atttattcac tggatattat cttaaacagt tcctctgctg caacttttgg
     4621 accgctggct tcataacccg gaatacccag ccgagagcgc atctgaccaa cacgaacacc
     4681 agcttcgaag gccttgacaa acacggtctc agccagtttg tcgtgcatgg cgcggtactg
     4741 tgggggaccg aagatattgg cgtaataatt atgccccaga ccagtggaag ttgtggttcc
     4801 tttggccata ctggcaccct cgcggaagac ctctatcgca cgtgaaggac tatcaaaaaa
     4861 ttcgatcgcc ggatggtctt ggtcaacctg ctcataattg ttggcataca gcagaatatc
     4921 caccggataa ccgtgcagaa cttcattaag cgtcgtaaca ggcagaacaa cacgggcatt
     4981 ggttttctgg ggactcataa taatcgaacg gtctaactgc tcgaaggcat atccggcctg
     5041 taaatcatcc aagcggataa aagccccgat ttccgtgccg tatcctatta aacgtccctc
     5101 cacatcaata tcaaaagaac ccatatcgtc tgcgataatc ttcatctggc agatttgatc
     5161 ccgtcccagc agtctgaacg cctcgagagt ttcggacttg ccggcagccg tatcgccgat
     5221 aataacaata gaggccgact ttccattctt aagggaaatt ctcaccatcg ccccgtgata
     5281 cggcattcgt ccccgcttca tcatcacaat attatgcagg gtcaaaatca tctttttgag
     5341 atagccaaaa taaccgaatt ggggggcatt ggggactgcc gcggcaagaa gccccgtgtc
     5401 ggcatcatca taaaacactg tcggattatc gctgtaccgc tgcatatgtt c
//
LOCUS       DAOTBO010000149         5356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12849_length_5356_cov_6.053575, whole genome shotgun sequence.
ACCESSION   DAOTBO010000149 DAOTBO010000000
VERSION     DAOTBO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12849_length_5356_cov_6.053575"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLE88_12040"
     CDS             <1..188
                     /locus_tag="PLE88_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77237.1"
                     /translation="SVPMDNELKIHRRRYLQVFADIGNSTPDEEYFGRREMILKRRAK
                     LETNAIEKQKGASFNDS"
     gene            697..3090
                     /locus_tag="PLE88_12045"
     CDS             697..3090
                     /locus_tag="PLE88_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM77238.1"
                     /translation="MYQGKAAVMASMAAVCCLLTVGCRMTTAREQISRSRDFCFDWKF
                     FNGDAAEAYQPAFDDAHWTDVHLPHDLSIEEFKDRTNPGRSENGYFAGGVGWYRKHFQ
                     LSPQEHGQKVFILFDGVYQNSEVWINGHSLGIRPYGYISFQHDLTPYVNWDGDNVLAV
                     RVNNTDQPNCRWYTGSGIYRRVWMIVTDKLYVRSWGTSITTPHVEPDKTYVRIAAELE
                     NAYADAQSCTLTTVILDPQGKVAAECASVQRIPAESGFQIEQELEVRCPQLWSIDSPA
                     MYIARTIVRNESGMCDQYDTPFGIRRIAFDKDKGFLLNGKPVKMKGVCLHHDAGCLGA
                     AVPVRVLQRRLEILKSIGCNAIRTSHNPPAPELLDLCDRMGFVVIDEAFDKWEGRYAR
                     SFAEWWQTDLTDMIRRDRNHPSVVMWSVGNEVTGQGSTKFMEQLKRMADLARRVDPTR
                     PVTCALFPHGANPAFIAQIADIVDVVSCNYMEQLFEDFRRERPDVILAASESFPYYRG
                     EGTNIKAFVPLNPWLEVEQHDYVVGGFYWAGIDYLGEAVAGWPYHGWNCCLIDTCGFV
                     RPIANLVKSFWIDQPMVHIAVMDDSLDVPKPVKEHWDWPKTDSHWNLSGQEGRQVKVA
                     TFTNCSAVELFVNAKPLGRKELKDFPDKMITWMVPYEPGHIEARGLEQGRTVCDHYLQ
                     TAGKPARIRLLADRRKIYADGCDVSHVEVQIVDEQGILVPDADSPIQFNIQGAGRIIG
                     VDNGDLTSMEPYKADRRKTFHGRALVIVQSLRQPGSITLKAQTAGLPPAQMILFAGR"
     gene            complement(3099..3172)
                     /locus_tag="PLE88_12050"
     tRNA            complement(3099..3172)
                     /locus_tag="PLE88_12050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3136..3138),aa:Val,seq:gac)
     gene            3661..4302
                     /locus_tag="PLE88_12055"
     CDS             3661..4302
                     /locus_tag="PLE88_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77239.1"
                     /translation="MRSSFKTTIVIASAGFTLSGVGGQQEILTINNSIPSSQSQLLDP
                     DNGLSKNIYNLRKRKGSFMKRNIYWVMIIILAIMPCAMGTTVITNVQVSPAQPSFFDI
                     ITIASYGSFSGGTNWYDKSEFTIDEQSLYLNLYFTGGFGPQITQPWSHNDVIGTLPQG
                     NYDLSVQAYWRVSGGANYILHDNYSISFEVIPEPLMLTFLVLGSFFVRRKCNK"
     gene            4340..4906
                     /locus_tag="PLE88_12060"
     CDS             4340..4906
                     /locus_tag="PLE88_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77240.1"
                     /translation="MSLISWFVAIILTMVAPFAYSMSQEKLNYKDDEVIIRFRDDTDK
                     TKTLREKKAFLDKKGGGKIIKSYKRLPNLHVIKLPKNLNVEDALKKYRDSKEVMYVEP
                     NYKIYLHSTIPNDPYFPQLWGLHNTGQEYPIEGGGTYYGIDDADIDATEAWDIYTGNQ
                     EIIVAVLDSGIDYNHPDLSTNMWVNQAR"
     gene            4971..5336
                     /locus_tag="PLE88_12065"
     CDS             4971..5336
                     /locus_tag="PLE88_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOM77241.1"
                     /translation="MIDEDSQGNSRYLEDGITPNPAYTNDIPGDDDENGYVDDIYGFD
                     FADYNSDPNDYYLHGTHCAGIIGAVGNNNIGVAGVCWNVKMMSVKIFPNYDRSESQSS
                     FMDIAIEGMVTSPIFRSID"
BASE COUNT         1482 a         1156 c         1354 g         1364 t
ORIGIN      
        1 agtctgtgcc tatggataat gagctaaaaa tccataggcg caggtattta caggtattcg
       61 cagatatagg caacagcacc cccgatgagg agtatttcgg acggcgggag atgattttga
      121 aacgcagggc caagttggag acaaatgcaa tcgaaaaaca gaagggtgct tcattcaacg
      181 attcatgaaa ccgcaagcca aaaccgtaca aataaagact ttaaattgtc tcatttcggc
      241 tgaagacgta cacacggttc aatccgggcg atattgtcga agacgggcgt attgacttgt
      301 tagacgctgc tgttttagct tggcgatgga aaaaagctcc ggggacgcct tctgccgaca
      361 tggcccccgc aggcggggac ggtcttgttg accaattgga tttgctgatg ctttgcagca
      421 attggccggc cgcggcagaa caatagggct gcagaaatag cccaacagca gcggtttaac
      481 ggcgcttgga ataggcggct ttcaggacag ccgcctgagg cattacaggg tattctgcag
      541 cttaagcact cgcagaatcc ggtatcaaac cttgcgtatg atagagattg catatctgcg
      601 cccaccttct ataataaaaa agagccggat tgcatgtgta ccagaaaggc atcggcagaa
      661 acggcttact gtaccagtca aggaaaggat aggcagatgt atcagggcaa agcggcggtg
      721 atggcaagca tggcagctgt atgctgttta ctgacagtgg gctgccgaat gacgacagcc
      781 cgcgaacaga tttccagaag ccgcgacttc tgttttgatt ggaagttttt caatggcgat
      841 gcagccgaag cctaccagcc tgcttttgat gatgcacact ggacggatgt gcatctgcct
      901 cacgacttga gtattgagga attcaaggac cgcacgaatc ccggcaggag tgaaaacggt
      961 tattttgccg gcggcgtcgg ctggtatcga aagcattttc agctttcccc gcaggagcat
     1021 ggccaaaagg tcttcatcct gttcgacggc gtttatcaaa acagcgaggt atggattaat
     1081 gggcattcct tgggcataag gccgtatggg tatatcagtt tccagcatga cctgacaccc
     1141 tatgtgaatt gggatggcga caatgtgctt gcggtgcggg tcaacaatac cgaccagccc
     1201 aactgccgct ggtataccgg ttccggcatc taccgccggg tatggatgat agtaaccgat
     1261 aaactttatg tccgcagctg gggaacttct ataaccacac cgcatgtaga gccggataag
     1321 acgtatgtgc gaatcgctgc ggaactggaa aacgcctatg ccgatgccca gtcctgtacc
     1381 ctgaccaccg tcattttaga cccccaaggt aaggttgcgg ccgagtgtgc gtctgttcag
     1441 cggattccgg ctgaaagcgg ctttcaaatt gaacaggagc ttgaagtgcg ctgtccgcag
     1501 ctgtggtcga ttgacagccc tgcgatgtac attgcccgaa caattgtccg aaatgaaagc
     1561 ggcatgtgcg accagtacga cacgccgttt ggaatccgcc gcattgcgtt tgacaaagac
     1621 aagggatttc tgctcaacgg caagccggtc aagatgaaag gagtctgtct ccaccacgat
     1681 gccggctgtc ttggggcagc cgttcctgtc agggtgctcc agcgccggct ggaaatcctc
     1741 aaatccatcg gctgcaatgc catccgcacc agccataacc ccccggcgcc ggaactgctg
     1801 gatttgtgcg accggatggg gtttgttgtc atcgatgagg cattcgacaa atgggaaggc
     1861 cgctatgccc gttcttttgc cgaatggtgg cagacggatt tgaccgatat gatccggcgg
     1921 gaccgcaacc atccgtcggt ggtgatgtgg agcgtgggca atgaggtgac cgggcagggc
     1981 agtaccaaat ttatggagca gctcaagcgg atggccgatt tagcccgccg cgtcgatccc
     2041 acgcggccgg tcacctgtgc cttgtttccg cacggtgcaa atccggcgtt tattgcacag
     2101 attgccgaca tcgttgacgt ggtttcatgc aattatatgg aacaattgtt cgaagacttc
     2161 cgcagggaaa gacctgatgt tattcttgca gccagcgaaa gcttccctta ttatcgcggc
     2221 gaaggcacca atattaaggc ctttgtgccg cttaatccat ggctggaagt ggaacagcac
     2281 gattatgtcg ttggcggctt ttactgggca ggcatcgact atctgggcga ggcagtggcc
     2341 ggctggcctt atcacggctg gaattgctgt ctgattgaca cctgcggatt tgtccgtccg
     2401 attgccaatc ttgtcaagag tttctggata gaccagccga tggtgcatat tgcggtaatg
     2461 gatgattcgc tggatgtgcc caagcctgtc aaggagcatt gggactggcc caagaccgat
     2521 tcccactgga atctgtcagg tcaggagggc aggcaggtca aagtggcgac ctttacaaat
     2581 tgttctgcag ttgagttatt cgttaacgca aagccgctgg gcagaaaaga actcaaggac
     2641 tttccggata aaatgataac ttggatggtt ccctatgagc ccggccacat cgaggcccgc
     2701 ggcctcgaac aaggcaggac tgtttgcgac cattacttgc agacagccgg caagccggca
     2761 cgaatacgcc tccttgccga tcggcggaaa atctacgccg atggctgcga tgtgagccat
     2821 gtcgaagtcc agattgtgga tgagcagggg atacttgttc ctgacgcaga cagcccaatt
     2881 cagtttaata tacagggggc aggacggatt atcggcgtag ataacggaga cctgaccagc
     2941 atggagccat acaaagccga taggcgaaag acctttcacg gcagagcact ggttattgtc
     3001 cagtcactcc gtcagccggg aagcatcacc ctcaaagcgc aaacagcagg attgcccccc
     3061 gctcaaatga tcctttttgc cggccgataa cttttcagtg ggcggtatag gacttgaacc
     3121 tacgaccccc agcgtgtcga gctggtgctc tagccaactg agctaaccgc cctaaagaac
     3181 ataagtcttt agtggataaa ctcatacact actttcaggc atttgtcaag aaggaaaatc
     3241 agaagaaaaa aggttccagc ggttctccag cgtcaaagac aaaagtgatt ggcgtggact
     3301 atgtgtggat tgggagcggg atttttgttg tggagagagg ggaaagaatc ccatatctac
     3361 ccacatctga caccctcaaa tctgcctttt caggattatt gtaaaagaac ttttaatctc
     3421 aacctaacac tatcaatata tattcctgta agtccttttc atgtctaatc ttaggattat
     3481 tatatataga atctttgaca taagcctcta aattttgcta taatatgaga agtattggaa
     3541 tgggagaagg ctacaggttt cattttcctc aaaggattgg ttctccccag ttattgttcc
     3601 tttacatcat gtaacgcgtt ctgaagcaat tgaacaagaa ttcgagcggg agagattctt
     3661 gtgagaagta gttttaaaac gacgattgta attgcatctg cgggctttac attatcggga
     3721 gtcggaggtc agcaggaaat actgacaatc aataattcta tcccttcatc ccaatcacaa
     3781 ctgctggatc cagataatgg tctgagcaag aatatctata atcttagaaa aagaaagggg
     3841 tcttttatga aaagaaacat ttattgggta atgattataa ttttggcaat aatgccttgt
     3901 gcaatgggca cgacggtaat tactaatgta caagtttcac cagcacaacc atcatttttt
     3961 gatattatca caatagcatc ttatggttct ttttctgggg ggactaattg gtatgacaaa
     4021 agtgagttca caatagatga acaatcactg tatcttaatt tgtattttac cggtggattt
     4081 ggaccgcaga ttacacaacc atggtctcac aacgacgtaa tcggcacttt gccacaggga
     4141 aattatgatt tatcagttca ggcatactgg agagtttcgg ggggagcaaa ttatattttg
     4201 catgataatt attctattag tttcgaggtt attcctgaac ctcttatgct tacatttctg
     4261 gttttaggtt ctttttttgt taggagaaaa tgtaacaaat aagccaacct tttaattatc
     4321 acacagaaag gagtctgaca tgtcactgat atcttggttc gtagcaatca ttttaactat
     4381 ggtagctcca tttgcatatt caatgtctca agagaaattg aattataaag atgatgaggt
     4441 gattattcga tttagagatg acaccgataa aacaaaaact ctcagagaaa aaaaggcgtt
     4501 tcttgacaaa aaaggaggtg gaaaaataat aaaatcatat aagagactcc caaatcttca
     4561 tgtcattaag ctacccaaga atctcaacgt tgaagatgcg ttgaaaaaat atagagactc
     4621 caaagaagtt atgtatgtcg agccaaatta caagatttac ttacattcaa caattcctaa
     4681 tgatccgtat tttcctcaat tatgggggct tcataacaca gggcaggagt atcctattga
     4741 aggaggtgga acttattatg gcatagatga tgcagatatt gatgcgaccg aggcatggga
     4801 catctataca ggaaatcagg agattatagt tgcagttctt gattcaggaa ttgactataa
     4861 tcatcccgac ttatcaacga atatgtgggt taatcaagcc aggtgatcca aacgatgcaa
     4921 acggagatgg ttatccgggc atagcgggca ttgatgatga cggagatggc ttgattgatg
     4981 aagattcaca aggaaatagt cgatacctcg aagatggcat tactccaaat cctgcataca
     5041 caaatgatat accgggagat gatgacgaga acggctatgt agatgatatt tacggatttg
     5101 actttgccga ttataatagc gaccctaatg attattactt gcatggaaca cattgtgcag
     5161 gaataatagg ggcagtaggg aataacaata ttggagtagc aggagtgtgt tggaatgtta
     5221 aaatgatgtc ggtaaaaata ttccccaatt atgatagatc tgaatcacaa tcctccttta
     5281 tggacattgc tattgagggg atggtaacat cccccatttt taggtccatt gactaagtta
     5341 ggatttccct gtaaag
//
LOCUS       DAOTBO010000150         5356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12850_length_5356_cov_5.241275, whole genome shotgun sequence.
ACCESSION   DAOTBO010000150 DAOTBO010000000
VERSION     DAOTBO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12850_length_5356_cov_5.241275"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLE88_12070"
     CDS             complement(<1..501)
                     /locus_tag="PLE88_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77242.1"
                     /translation="MRILYLTHFFQPEPIFWALPLAKELSRKGHQIEVLTGFPNYPAG
                     KLYDGYRIRMLQREVLEGIDVIRVPLYPSHDNSAWRRILCYTSFGLSASLIGPWAAKQ
                     ADIVYLMQGPMTLGLPALILKLFKRIPFVIHIQDIWPDSLLSTGMFNSKIGLKLLNGW
                     CNFVYKH"
     gene            complement(567..1697)
                     /gene="wecB"
                     /locus_tag="PLE88_12075"
     CDS             complement(567..1697)
                     /gene="wecB"
                     /locus_tag="PLE88_12075"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOM77243.1"
                     /translation="MKVLTILGTRPEIIRLACTMTLLDKHLEHKIVHTGQNYDYELNE
                     IFFNDLNVRKPDHFMNVDTSSLGKVYGGILIKAEEILKQEKPDAVLILGDTNSSIAGI
                     MAKRLKIPIYHMEAGNRCFDLNVPEEINRRIIDHISDFNLCYTEHARRHLLSEGLPHR
                     RIYVTGSPMREVLNIHLERIKASKALQELGLDKGKFFLVSIHREENVDRREGLTALID
                     AFEKLASEFNWPVIVSTHPRTRKRLETLGRKNISDKIRWMKPFGFLDYVNLQMNAACV
                     LSDSGTICEESSMLGFPAVTVRTAMERPEAMDTGSIVLTGLDADVIVKSVKVQMDEVG
                     SQGLCVPDDYQITNTSHRVLNIILGTAKLSNAWHGIQYNDLV"
     gene            complement(1701..2822)
                     /locus_tag="PLE88_12080"
     CDS             complement(1701..2822)
                     /locus_tag="PLE88_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOM77244.1"
                     /translation="MMTNNRIGITGMAGFIGTHLRDRLMREKDIEVLPFEDAYFDNPE
                     AFVDFCKKADVIVHLAGVNRHEPQIVYEKNIELMEKLLQFVNNAGKKPYILFSSSTQI
                     ERDNEYGRGKKRAMELLEAWSNKHHLPAVSMVIPNVFGDGGRPFYNSVVATFCYQLTH
                     GQTPTIQADSQMKLIYINDLVEDIWRLIQNPPNGYHIEYIKPRAEAKVSEILNWLNQF
                     KEAYYGSGTVPEQKTDFQRDLYNTFLTYMDAADWERKLKLNTDERGSFVEVFKLERGG
                     QVSFSTTKPGITRGNHYHIRKNEKFCVISGQASIKLRRIGTDKVIEYKVSGDSPSWIE
                     MPIYYTHNITNIGSTELVTLFWISEHFNPDDPDTFFEKV"
     gene            complement(2897..3934)
                     /locus_tag="PLE88_12085"
     CDS             complement(2897..3934)
                     /locus_tag="PLE88_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOM77245.1"
                     /translation="MARLKSPSLTGKTILVTGGTGSFGKAMVNRLLKTKVKQIRIFSR
                     DELKQELMRIDYGESRLSFYIGDVRDRASVDNVMKGVDYVFHAAALKQVPSCEFFPMQ
                     AVMTNIIGGHNVIESAIAHKVKSVVCLSTDKAVYPINAMGMTKSLMEKVAQAVARTSA
                     CNQTVVSCVRYGNVMCSRGSVIPLFIDQIKKGKPLTITVPEMTRFLLPLRDSVDLVLF
                     AFTHARPGDIFVKKAPACTVQDLAQALLNIFKSKLPIRIIGIRHGEKLYETLVTKEEM
                     VRAEDMGDYYRIALDDRNLNYALYFTQGDKKEMQLDDYHSHNTKRLNVKQVEQLLLTL
                     PEVTDELKNWK"
     gene            complement(3957..4898)
                     /locus_tag="PLE88_12090"
     CDS             complement(3957..4898)
                     /locus_tag="PLE88_12090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM77246.1"
                     /translation="MEDVERIPRCGDLSRFFCLSNWIVRLICKLRRIPVLDWTQGVKR
                     PEKGIRWWLADLYYRQADAILFYGNFARDYFIKKGYPAKKLFVVYNSLDYDTQKLVLE
                     SITPEAAARCRIEFGVVNPEDRLIINSSRLERGKQIDLLIRAVAMLKQMGHRVYAVLV
                     GDGQERSALETLARELSVADRVRFFGACYQEDKLGLLFAAADVCVMPGPVGLIAMHCL
                     VYGTPLLTCENSQGRHGPEIETIVEGKTGRFFRQGDAADLAEKMKQMLYPIPCKEHMS
                     AACMDIIDRYYTPQYQEKVILEALNSVLPKDKQIINS"
     gene            complement(4841..5050)
                     /locus_tag="PLE88_12095"
     CDS             complement(4841..5050)
                     /locus_tag="PLE88_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77247.1"
                     /translation="MTFEVCAGSNSNIGGGKRLACVVKPEQAEGIRWCLLRSYRLGRW
                     EWQPGAVWKMLKESPDAVICHVFFV"
BASE COUNT         1387 a         1416 c         1149 g         1404 t
ORIGIN      
        1 atgcttgtac acaaaattac accatccatt cagcagtttt aaccctattt tactattgaa
       61 catccctgtc gaaagcaatg aatcaggcca tatgtcctga atatgaatca caaacggtat
      121 tcttttgaac agtttcagta tcagtgccgg cagcccaagc gtcatcggac cctgcatcag
      181 atagacaata tccgcttgct ttgctgccca aggtccaatc agggatgccg aaagcccaaa
      241 actggtatag cataagatac gcctccatgc agaattgtca tggctgggat acagcggaac
      301 acgaatgaca tcaatcccct ctaaaacttc ccgctgaagc attcgaatac gatagccatc
      361 atataatttt cccgccggat aattcggaaa accggtcaat acttcaatct gatgtccctt
      421 ccgcgacagc tctttggcca gcggtaatgc ccaaaaaata ggttccggct gaaaaaaatg
      481 agttaaatat aaaatgcgca taagcaaaaa ctgccccgat aggtgaggag ttcgctgagt
      541 tcattatgtt cagcaaattt catggactag acaagatcgt tatattgaat gccgtgccag
      601 gcattggaca gtttagccgt gccgaggatg atattcagta cgcggtggga ggtgttggta
      661 atctgataat catccggcac acagagacct tgggagccga cttcgtccat ctgaactttg
      721 acggatttga caatcacgtc cgcatccaac cccgttaaaa caattgagcc ggtatccatt
      781 gcttccggtc tttccatagc ggtacgcacc gtgaccgctg gaaagcccag catcgatgat
      841 tcttcgcaaa tcgtaccgct gtccgacaaa acacaggcgg cattcatctg caaattcaca
      901 taatcgagaa aaccaaaagg tttcatccag cgaatcttat ccgagatatt ttttctgccg
      961 agggtttcca gacgctttcg ggtgcgggga tgcgttgaga caatcacagg ccagttgaac
     1021 tccgatgcca gtttttcaaa ggcatcaata agagccgtca gtccctctcg gcggtccaca
     1081 ttctcttcgc gatggatact gactaaaaag aatttgccct tgtccagtcc cagctcctgt
     1141 aatgccttgg aggccttgat gcgctccaga tgtatgttta aaacctcacg catcggcgaa
     1201 cccgttacat aaattcgccg gtgcggcaaa ccttcggaca ataaatgccg tcgggcatgt
     1261 tcggtatagc acagattaaa atcactgata tggtcgataa tccgacgatt gatttcctca
     1321 gggacattga ggtcaaaaca gcggttgccc gcctccatat gataaatcgg gattttgagc
     1381 cgcttggcca taataccagc aatcgaactg ttggtatcgc ccagaatcag gacagcatcg
     1441 ggtttttctt gctttaagat ttcctcagcc ttaatcaaaa taccgccgta gactttgcct
     1501 aagctggaag tatcgacatt cataaagtga tcaggcttgc ggacatttaa atcattaaag
     1561 aaaatctcat tcagctcata atcgtagttc tggccggtgt ggacaatttt atgttcgagg
     1621 tgcttatcca gcaatgtcat cgtacaagcc agccgaataa tctctggacg cgtaccgaga
     1681 atggtgagga ccttcatagt tcagaccttt tcaaaaaatg tatccggatc atccggattg
     1741 aagtgttcgc ttatccaaaa cagagttaca agctctgttg agccgatatt agtaatatta
     1801 tgcgtataat aaatcggcat ctcaatccac gacggcgaat cgccgctcac tttatattca
     1861 atgactttgt ccgttcctat ccggcgcagc ttaatgctgg cctgcccgct gattacgcaa
     1921 aacttttcat tcttacggat atggtaatga ttgccgcggg taatacccgg cttggtggtc
     1981 gaaaaggata cctgcccccc cctctcaagc ttaaacacct ctacaaagct gccgcgttca
     2041 tccgtgttca gtttaagttt gcgttcccaa tctgctgcat ccatataagt caaaaacgta
     2101 ttgtacaaat cccgctggaa atccgttttt tgctccggca ccgtcccgct gccataataa
     2161 gcctctttga actgattgag ccaattgaga atctctgaga ctttggcttc ggcacggggc
     2221 ttgatgtatt ctatgtgata gccattcgga ggattttgga ttaaacgcca aatgtcttcg
     2281 accaaatcat tgatataaat cagcttcatt tggctgtccg cctgaatagt cggtgtttgg
     2341 ccgtgcgtga gctgatagca gaaggtagca accacagaat tgtaaaaggg tctgcccccg
     2401 tcgccgaata cgttggggat aaccatcgag acagcaggca gatgatgctt attcgaccat
     2461 gcttccagaa gttccattgc ccgttttttg ccgcggccgt attcattgtc cctttcaatc
     2521 tgcgtgctgg aggaaaagag gatatagggc ttttttccgg cgttattaac aaactgaagc
     2581 agtttttcca tcagttcgat atttttttca taaacaatct gaggttcgtg gcggttgacg
     2641 ccggctaagt ggacaatcac atcggccttt ttgcaaaagt caacaaacgc ctccggattg
     2701 tcaaaatagg catcttcaaa cggcaacact tcaatatcct tttcccgcat cagccggtcc
     2761 cgcaggtgag taccgatgaa gcctgccatt ccggttattc cgattcgatt atttgtcatc
     2821 atcgtttcct gttaagagtg tgctttgggc cgtcagctgt tagcagcctg ctacgggctg
     2881 accgctaaaa gccggtttat ttccaatttt tcaattcatc cgttacctcc ggcagtgtta
     2941 agaggagttg ttcgacctgt ttgacattca gccgtttggt attgtgggaa tgataatcgt
     3001 ccagctgcat ttcttttttg tcgccctgtg taaagtacaa cgcatagttc aaattgcggt
     3061 cgtccagtgc gatgcggtaa taatccccca tatcttcggc acgcaccatt tcttctttgg
     3121 tcaccagcgt ttcatagagc ttttcgccgt gtcggatacc gataattcgt ataggcaatt
     3181 tggacttgaa tatattcagc agcgcttgtg ccagatcctg aacagtgcag gccggcgcct
     3241 ttttgacaaa aatatcaccc ggccgggcat gggtaaaggc gaacagcacc aaatccaccg
     3301 aatcccgcag cggcagcaaa aaccgcgtca tctccggcac ggtaatcgtc aggggtttcc
     3361 ctttcttgat ttggtcaata aacagcggaa tcaccgaacc tcgggaacac attacattgc
     3421 cataccgcac gcacgacacc accgtttgat tgcacgcaga cgtacgagca acagcctgtg
     3481 caactttctc catcagcgat ttggtcattc ccattgcatt aatcggatag accgctttgt
     3541 cggtgctgag gcaaacaacg cttttgacct tgtgggcaat tgccgactcg atgacattat
     3601 gaccgccgat gatgttggtc atcaccgcct gcatcgggaa aaactcgcac gacggcacct
     3661 gcttcagcgc tgcagcgtga aagacataat ccactccctt catcacatta tcgacactcg
     3721 cccggtcacg gacatcgccg atatagaacg acagccggga ctccccgtag tcgatgcgca
     3781 tcagttcctg cttcagttca tcgcggctga aaatgcgaat ctgcttaact ttggtcttga
     3841 gcagccgatt gaccatcgcc ttgccgaacg accctgtccc gccggtaacg agaatggttt
     3901 tgcctgttaa agacggggac tttaacctcg ccatacaaac ctcctataga tttttattaa
     3961 gaatttataa tttgtttatc tttcggtaat accgaattca gtgcctcaag aattactttt
     4021 tcctgatact gcggcgtata gtagcggtcg atgatgtcca tacacgccgc cgacatgtgt
     4081 tctttacatg gtatcggata cagcatttgt ttcatttttt ctgccaagtc cgccgcatcc
     4141 ccctgccgga aaaatcggcc ggttttgccc tcaacaatcg tctcaatctc cggcccgtgt
     4201 ctgccctgcg aattctcgca cgtcaatagc ggtgtaccgt acaccaagca gtgcatcgca
     4261 atcaacccta ccggacccgg catcacacat acatctgccg ctgcaaacag aagccccagt
     4321 ttatcctcct gatagcaggc gccaaaaaaa cgtacccgat ccgccacact cagttcccgc
     4381 gccaatgttt ctaaggccga ccgctcttgt ccatccccca ccaaaacagc atacacccga
     4441 tgccccatct gtttgagcat agcaaccgcc cgtatcagca gatcaatctg tttgcccctc
     4501 tccagacggc tggagttgat tatcaatcga tcctctggat tgaccactcc aaactcgata
     4561 cgacaccgcg ctgccgcctc aggtgttata gactcgagaa caagtttttg tgtatcgtaa
     4621 tccagcgagt tgtacaccac aaaaagcttc ttggcgggat atcccttctt gatgaaataa
     4681 tcccgggcaa aattaccata aaacaaaatc gcatctgctt gtcgataata aaggtcggcc
     4741 agccaccaac gaatcccttt ctcaggccgc ttgacaccct gtgtccaatc caacaccgga
     4801 atacgccgaa gcttgcagat caggcgcact atccagttgc tcagacaaaa aaaacgtgac
     4861 aaatcaccgc atcgggggat tctttcaaca tcttccatac tgctccgggc tgccattccc
     4921 aacgccccaa acggtaagaa cgcagtaaac accaacgaat accctcggcc tgttcgggct
     4981 tgaccacaca cgccagccgc ttgccgccgc caatattcga attgctgcct gcacagacct
     5041 caaatgtcat ttggggatgc tgcgacaaac gccgaaagat tggtatccga tagtgcggaa
     5101 tacaccaaat aaaatgaaaa accgtgggtt ttgatacttg cggtcggact gacatggcta
     5161 tagtttttca atctcaccca gcatttgtga aacaatctgc tcccacgact tgaaaaatgc
     5221 tgaaatcctt tggcatccct ttccagaaac ttgcttgcgc ataggttcag agtctcaaga
     5281 ctttttctgc cacttttgta atagtaaact ttcgcacttt ttaggcgact aattttaatg
     5341 gtttatcaac aaaaac
//
LOCUS       DAOTBO010000151         5344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_12884_length_5344_cov_5.908300, whole genome shotgun sequence.
ACCESSION   DAOTBO010000151 DAOTBO010000000
VERSION     DAOTBO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12884_length_5344_cov_5.908300"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLE88_12100"
     CDS             <1..383
                     /locus_tag="PLE88_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOM77248.1"
                     /translation="YVFFRTDSGNPRGCLNEPVLPMRTIATDKSIYPRACLAFLNTRL
                     PRPMADGTIQKAPYTGFALDQDAGGAIRAPGRCDVYMGIGEQAGRMAGQVYEEGKLYY
                     LFLRPDLVGQYLTPQGTADKTKAQ"
     gene            400..1818
                     /locus_tag="PLE88_12105"
     CDS             400..1818
                     /locus_tag="PLE88_12105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOM77249.1"
                     /translation="MNFQLRFLGAAESVTGSRHLLEFNHTKVMVDCGLYQERDLQYRN
                     WEDFGVHPADVDAILLTHAHLDHCGLLPKFVKEGFKGKIFCTEATAQLAKIVLADAGK
                     IQEEDAEFKRKRHKKANYTPPRPVEPLYTIQEAEACFPMFKAVAFEETVSVADGIKVS
                     FHQGGHILGSSIIQIVLSFNGQNRTVLFTGDLGRVGKPILKDPAVFDKADYILVESTY
                     GDRQHTSTEDTKEQLYQAVIDTINAGGNIVVPSFSIERSQEVLYYMNELLMEDRIPHI
                     MTFLDSPMAVKVTDVFKKHPELYDAQMARLMEQNESPFSFRGLKLIESTRESKAINHI
                     RGTVMIIAGSGMCTGGRIKHHLVNNITRPESTILFVGYQASGTLGRTILDSKPGQEVR
                     ILGQMYPVRAKIVRVHGFSAHADREEILAWLKNFKTPPKKIFIVHGEKETSESFKDYL
                     IQQTGWSVMVPKYQDVVKINGI"
     gene            2072..3037
                     /locus_tag="PLE88_12110"
     CDS             2072..3037
                     /locus_tag="PLE88_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOM77250.1"
                     /translation="MTERIPKIVVVGPAFVDMAVKCDEHPMPGKIAEGSGFTCTPAGA
                     GVMEATQVALCGCETYLLTRVGEDCFGEMIIQNLKRHSVQTDLVYPTQAMSTGVILTL
                     VNRKGENISCRSQGAARILSRDEIEYAAAEQQIGSADAVLVNDAVPRAASVAVIRSAQ
                     IHKTRVVLSAKLPQPSRDAVQSLDWPMEFYNTDILVVRFKGMMCGTELGAGGEGDLKF
                     IGTELVARGAACVVISLGWRGALIMDRQGPRHLPGIAAEVVDQNGCDSAFTGALTACY
                     GTGDTADNAVRFAIAAEALMRSRFGLQEALPKKEEVLTLLQSQPD"
     gene            complement(3119..>4460)
                     /locus_tag="PLE88_12115"
     CDS             complement(3119..>4460)
                     /locus_tag="PLE88_12115"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM77251.1"
                     /translation="KTGTDSFTYTLLDGFGGSDTAVVTINLTNADPVAYDNSYGVSHN
                     VQLTVSSTDGTITGILNGAAADYDPDNANAGKLFDDVLTVVPLTNVPTANGGTVTLQA
                     DGSFVYTPSPDNKTGTDSFTYTLLDGFGGSSTAVVTITLSNQLPIARPDNYGTQQDVT
                     LIVTKPNDVITGVIPDINGDYDPDNSNEGKVFDDVLNAVLAGDGTTALGGKVILNGDG
                     TFTYIPPAGQSGTDSFTYFVTDGYGNSNTVVVTIAIEGSFAVPAAPLPVLELPQLKGC
                     PVVMDAVAAELGVHSDQLQMMIRNSLAANPNLQPCEACENLLNAAATLKGIDAERLGA
                     MNQIFNTLAPADAPFTPEVRASVQMAFEGLAAREIQLAEMTPDEYAQYQQYAMTNEII
                     EAFVRYVAVLNSDLKLPIGNPVAMAVEKYGASITNSENPNMAAYIIARMEAAMQ"
     assembly_gap    4461..4560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4561..>5344)
                     /locus_tag="PLE88_12120"
     CDS             complement(<4561..>5344)
                     /locus_tag="PLE88_12120"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM77252.1"
                     /translation="NTEEVPPADPASPCVDCPTPPFLPPLPPILLILDDAYLINNKYV
                     VNFGLDVLGNDGPNETALDGEIIWWSNGTSGTITPIYDASDPTKIIGFEYTPNLDTIV
                     FEWDGTSDYASFTDTFRYKAKDADGNISLNKATVTIEVRNLLPVAQPDEYTESHNVLL
                     QADGTPAGVIEGMIPAASGDYDLDAGDTLKAILVDGPVWGTVVLNPDGTFVYTPSAGN
                     TTGTDSFTYKVTDGFNESNTTTVTLNLTNADPVAYDNSYGVSH"
BASE COUNT         1229 a         1286 c         1443 g         1286 t
ORIGIN      
        1 gctatgtctt tttccgcacc gacagcggca atccgcgggg ctgtctgaat gaacctgttc
       61 tgcctatgcg tacgatagcc acggataaat cgatttatcc gcgggcatgt ctggcgtttc
      121 tgaatacccg gctgcctcgc ccaatggccg acggcacgat acaaaaagcc ccctataccg
      181 gttttgcact cgaccaagat gccggcggag caattcgggc gccggggcgc tgcgatgtct
      241 atatgggtat tggcgaacag gccggacgaa tggctggtca ggtctatgaa gaaggcaagc
      301 tgtattatct tttcctgcgt cctgaccttg tcggccaata cctgacccca caggggacgg
      361 cagataagac aaaggcacaa tagtggttga aaggcatata tgaattttca gctacgattc
      421 ttgggtgccg cagaatcggt taccgggtct cggcatttgc tggagttcaa ccataccaag
      481 gtgatggttg attgcggctt gtatcaggaa cgcgacctgc aatatcgcaa ctgggaagat
      541 tttggcgtcc atcctgctga tgtcgatgca attcttttaa cccatgctca tctggaccac
      601 tgcggtcttc tgccgaagtt tgtcaaagag ggatttaagg gaaagatttt ctgtacggag
      661 gccactgccc agttagccaa gatcgtcttg gctgatgcag gcaagattca ggaagaagat
      721 gccgaattca agcgaaaacg gcacaaaaag gccaactata ctcccccccg gccggtcgaa
      781 cccttatata ctattcagga agccgaggcc tgtttcccga tgtttaaggc ggttgccttt
      841 gaagaaaccg tttctgttgc ggacggcatc aaggtcagtt tccatcaggg cggacatatt
      901 ctgggttctt ctattattca gattgtctta tcgttcaatg gccaaaaccg aacggtttta
      961 tttacgggtg atttagggcg ggtgggcaag ccgattctca aagatcctgc cgtcttcgat
     1021 aaggccgact atattctcgt tgaatcaacc tacggcgacc ggcagcacac cagcaccgag
     1081 gataccaaag aacagctgta tcaggccgtt attgacacaa ttaacgccgg cggcaatatt
     1141 gttgttccca gtttttccat cgagcgaagt caagaggttt tatactatat gaatgagctg
     1201 ctgatggaag atcgcatccc ccatattatg acttttttag acagcccgat ggcggtcaag
     1261 gttaccgatg ttttcaagaa gcaccctgaa ctttatgatg cccagatggc ccggctgatg
     1321 gaacagaatg aatcgccgtt ttccttccgc ggccttaagc tgattgaatc cacacgggaa
     1381 tccaaagcca tcaaccacat ccgcggcacg gtaatgatta tagccggttc ggggatgtgt
     1441 acaggcggac gcatcaagca ccatctggtt aataatatca cccgtcctga aagcacgata
     1501 ctttttgtcg ggtatcaggc gtcaggcact ttggggcgaa cgattctgga ttccaagccc
     1561 ggtcaggaag tccgcatatt gggtcagatg tatcctgtcc gtgcaaagat tgtacgtgtt
     1621 cacggctttt cagcacacgc cgacagggag gaaatacttg cttggctgaa aaatttcaaa
     1681 actccgccta aaaaaatctt cattgtgcat ggggaaaaag aaacttccga aagtttcaaa
     1741 gattatttga tacagcaaac cggctggagt gtaatggtcc ccaaatacca agatgtcgtc
     1801 aagatcaacg ggatttaata cctctttttt tcatcgtatt ttatcattca ttagcgaaaa
     1861 catctgtatt ctggcgatat aacatatcca gatactccct tcgccgatat atatcttcca
     1921 gctgaaatgc ttctgcggcg ggattcatcc aaaacctaag acagccctac cgtatcgtat
     1981 aaccttgcag ggcgaaaaaa tggaaaaatc ttttcaaaat caggattgtc ggatatagta
     2041 acggacacac gaggtattga agggaatagg tatgactgaa cggattccga agattgttgt
     2101 ggtcggtccg gcttttgttg atatggctgt taaatgtgat gaacacccaa tgccgggcaa
     2161 gattgcagaa ggaagcggtt ttacctgtac gccggcgggt gccggggtga tggaagccac
     2221 acaggtagca ctttgcggtt gtgaaaccta tctgctgacc cgggtcgggg aagactgttt
     2281 cggggaaatg ataatacaga atctcaagcg gcactcggta cagaccgatt tggtctatcc
     2341 cacgcaggca atgagcacgg gtgttatttt gacgcttgtc aaccgcaaag gagagaatat
     2401 cagctgccgc tcgcaaggag ctgcccgcat tttaagccgc gatgagattg agtatgccgc
     2461 cgccgaacag caaatcggct cggcggatgc ggtgctggtg aatgatgcag tgccccgggc
     2521 tgcttcggtg gctgtaatcc ggagcgcaca aattcacaaa acccgtgtgg tgctgagtgc
     2581 caagctgcct cagccaagcc gtgatgccgt gcagtccctc gactggccga tggagtttta
     2641 caataccgat atccttgttg tgcgttttaa agggatgatg tgcggcaccg aactgggagc
     2701 ggggggcgaa ggcgatttga aatttatcgg taccgaactg gttgccagag gagccgcctg
     2761 tgttgtcatc agtcttgggt ggcgtggcgc attgataatg gatcggcagg gaccccgtca
     2821 tctgcccggc atagcggcag aagtggttga ccagaatgga tgcgattccg catttaccgg
     2881 cgctctgacg gcctgctacg gcacgggtga cacggccgat aatgcggtgc ggtttgccat
     2941 cgccgctgaa gccctgatgc gaagccgttt cggccttcag gaagccctgc ctaaaaaaga
     3001 agaggtcctg acacttctgc agagtcagcc ggattaagcg gtctggtatg acccaacaga
     3061 aaaaaaccct tggagtctgg gcgacttcca agggtttttt catagctgtt cagcccgctt
     3121 actgcattgc tgcttccatc cgggcgatga tataagcggc catattcgga ttttcgctgt
     3181 tggttatcga agctccgtat ttttccacag ccattgcaac aggattgccg ataggcagct
     3241 tcaggtcgct gttgagaaca gcaacatacc ggacgaaggc ctcgatgatt tcattggtca
     3301 ttgcatattg ctgatattgg gcatattcat cgggggtcat ttcggccagc tggatttctc
     3361 tggctgccag accttcaaat gccatctgga cagaggccct gacttccggt gtgaaggggg
     3421 catcggcagg agccagcgta ttgaatatct ggttcatagc ccccagccgt tctgcatcga
     3481 tgcccttcaa tgtagctgcg gcattcagca gattttcgca tgcctcacac ggctgcagat
     3541 tgggattggc cgccagcgaa tttcggatca tcatctgcag ctggtctgaa tgaacgccta
     3601 attcagcagc gacagcatcc attacgaccg gacagccctt cagctgcggc agttcgagta
     3661 ccggcagagg cgccgccgga acagcaaaac tgccctcgat tgcaatggtt acgacaacgg
     3721 tatttgaatt gccatacccg tcggttacaa agtaggtaaa gctgtccgtt ccggattgac
     3781 cggccggcgg gatataagtg aaagtaccat caccgttgag aataaccttg cccccaagcg
     3841 cggttgttcc atctccggcc aataccgcgt tcagcacatc atcgaacact ttaccttcat
     3901 tgctgttgtc aggatcatag tctccgttaa tgtcaggaat gacgccggta attacatcat
     3961 tgggttttgt tacaatcagc gtaacatcct gctgggtgcc gtaattgtcc ggacgggcta
     4021 tcgggagctg gttcgacaat gtaatggtaa ccaccgcggt actgctgccg ccgaagccgt
     4081 ccagcagggt ataggtaaac gagtcggtgc cggtcttgtt gtccggcgag ggggtataga
     4141 caaacgagcc gtcggcctgc agggtgacgg tgccgccgtt ggcggtgggg acattggtca
     4201 gaggcacgac ggtcaggaca tcatcaaaga gcttgccggc attggcgttg tcggggtcat
     4261 agtcggccgc tgcgccgttg aggatgccgg tgatggtgcc gtcggtgctg ctgacggtca
     4321 gctggacatt gtggctgacg ccgtagctgt tgtcataggc aacggggtcg gcgttggtca
     4381 gattgatggt aaccaccgcg gtatcgctgc cgccgaagcc gtccagcagg gtataggtaa
     4441 acgagtcggt gccggtcttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 tgtggctgac gccgtagctg ttgtcatagg caacggggtc ggcgttggtc agattcagcg
     4621 ttactgttgt cgtatttgat tcattgaagc cgtcggtaac tttgtaagta aaggaatctg
     4681 tcccagtcgt attgccggct gagggtgtat aaacgaatgt cccgtcggga ttgagaacaa
     4741 ccgttcccca gacgggtcca tcaaccagaa tggctttcag tgtatcgcct gcatcaagat
     4801 catagtcgcc gcttgcagcc ggaatcatgc cttcaataac gccggccggc gtgccgtcgg
     4861 cttgcagcag aacattgtgg ctttctgtgt actcatccgg ctgtgcaacc ggaagcagat
     4921 ttcgcacttc gatggttaca gttgccttat tcagagaaat attgccgtcg gcatcttttg
     4981 ctttataccg gaatgtatcg gtaaaggatg cataatcgct ggtgccgtcc cattcaaaaa
     5041 cgattgtgtc caaatttggc gtatactcaa aaccgataat cttcgtcggg tcagaggcgt
     5101 cataaatggg tgtaatcgtg ccgctggtgc cattgctcca ccaaattatc tcgccgtcca
     5161 gcgccgtctc gttcggaccg tcattgccga gaacatccaa accaaaattg acaacatatt
     5221 tgttgttgat cagatacgca tcgtccagaa tcagcaatat tggcggcagt ggcggaagga
     5281 acggcggggt tgggcaatcc acgcagggac tggctggatc ggcagggggc acttcttctg
     5341 tatt
//
LOCUS       DAOTBO010000152         5262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_13134_length_5262_cov_3.528519, whole genome shotgun sequence.
ACCESSION   DAOTBO010000152 DAOTBO010000000
VERSION     DAOTBO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13134_length_5262_cov_3.528519"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PLE88_12125"
     CDS             <1..1321
                     /locus_tag="PLE88_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOM77253.1"
                     /translation="VRRAAASNIDLKIAESRLREARARLTMVSGNRAPQVGFTSEYSR
                     EQISHTAYPTGPLAGDESGIPLRWNLYDFGFDASWELDVFGGLKRSVEAAQADMGAMI
                     EDRRDVLVSTAAEIARNYVELRGLQHEYQIAQRNLAVQQQTLELIMDQKNKGTATQLD
                     VDRAAAQVSATKAEMPTLQERQWQSMHRLAVLLGAGPEDLVEELSPAAPIPVPPQHIG
                     IGLPSDLLRRRPDIRRAERQLAASTARLGVAAADLFPRFSLTGSAGLQSSNTNNLLEG
                     DSNRYFFGPSLNLPIFNGGQLHAMVKVRTEQQEQCLMQYQQTVLTALREVEDAIVAFR
                     LEQQRRQFLQETVSSNQEAEELAQQLYRNGLTDYLTVLDAQRSLYKSQEMLAVSEKKV
                     TINLIVLYKALGGGWEPESAPVETPETMPPAMVSHSVSQKTVLDVTP"
     gene            1371..2756
                     /locus_tag="PLE88_12130"
     CDS             1371..2756
                     /locus_tag="PLE88_12130"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOM77254.1"
                     /translation="MAHEAGTHSQQTPNREADTFVKNTSQKGKMDIPPYSRPFYKRPF
                     WMAIVILMSVLIVTAVVCYWLHRRQFESTDDAFIDGDIVQVSAKVPGIIESVYVDDNQ
                     DVAAGTVLLQIDPKDLQARLNEARASLMAAEAKLGEAKAQVDVVKAATDALLTQAKAG
                     VEQAKAAVESSISQLASAQSDVVAAEAEAVKRQADLKRFKSLDPRVVSQQQLDSAQAA
                     ADTAEAYLVSTRKRVLSAEAAVKEAQAKYAQSLAVLAAAETGPQQVAAAVAQMENARS
                     GVDEAKAKVDAAELNLSYTTIRAPVSGRVSKRTARPGQYLQVGQNVLALVQPDVWVTA
                     NFKETQLTCMCQGQNVEIEVDAYPGHVFHGHVESIQAGTGSRFSMLPPENATGNYVKV
                     VQRVPVKIRFDQKDTQQRLLAPGMSVIPTVRVGRDKQQHPQPIMPPSEPAVSEAADLS
                     PKADCPSEVAQ"
     gene            2753..4324
                     /locus_tag="PLE88_12135"
     CDS             2753..4324
                     /locus_tag="PLE88_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOM77255.1"
                     /translation="MSRPYPSAQQTADQPASTAHPWLIAVAVMSPTVMVVLASTVVNV
                     ALPYIAGTVSAGTEEVTWVITSYLAANAVIMPATGWLSRLFRRKRFVMGCILTFSAGS
                     LACGMAHSLGFLVAARIIQGLGGGAMQPVSQAVLLESFPPAKRAVAMAIYGVSVIVAP
                     IVGPILGGWITDNYSWRWVFYLNLPIGLISLLMVYLFVEDPPYIRQGTARVDYIGLIL
                     LVIWVASLLIMLDKGQQEDWLDSRFIRTLLVMFILGLTAFIFWELQVREPVVNLQILC
                     NPTFTIGTTINTVASGAVLFSYTAYLPLFLQTLLGYPALRSGLTMAPRGLGALITMFL
                     VGQLISVVSPRLMLAAGFLTLGYSLYAVGNLNLEISPRNVVIPNFLSGTASGLIFVPL
                     TTITMGNLRREEVANATGIYNLLRNIGSAIGIAVMATLMARGAQVHQTNLVAHMTPYD
                     TVYQQKLESIQSALTPQSGACTAAEQAVAVLGKELLRQSTLLSYVDNFRYLALASVAC
                     VPALLFLSRQRLKSG"
     gene            4367..5047
                     /locus_tag="PLE88_12140"
     CDS             4367..5047
                     /locus_tag="PLE88_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOM77256.1"
                     /translation="MEDDSIMSKKPFIAITIARQMGAGGLVVGRSVARRLGYAYLDRA
                     ILRMVARQLGITDKELEHWDEHVSTFWERLSRVLLIGPAEATYTGVPQAAGIRDRQLF
                     LLESRVIREAAFSRNVVVIGRAGFDVLRDHPGLASVYLHAPLPARIDIVMRTHHLTEP
                     AKARELIERTDADRIRFVRDMTGRLAYDANCYHLSINTDRTGLALAEEMIVNLVEHVQ
                     QMLAGNSQ"
BASE COUNT         1192 a         1423 c         1454 g         1193 t
ORIGIN      
        1 ggtaaggcga gcggctgctt cgaatatcga tttgaaaata gcagaatcac gcctgcgcga
       61 ggcaagggcg agattgacaa tggtaagcgg gaatcgagca ccgcaggtcg gttttacctc
      121 ggaatactca cgcgagcaga tcagtcatac cgcctatccg accggtccct tggccggcga
      181 tgaatccggc atcccattaa gatggaatct ttatgatttc ggctttgatg cctcatggga
      241 acttgatgtg tttggcgggc tcaaacgcag cgtcgaggcc gcccaggcgg acatgggggc
      301 aatgatagaa gatcggcggg atgtactggt cagcacggca gccgaaatcg cacgcaatta
      361 tgttgaactt cgcggtctcc agcatgagta tcaaattgcc cagcggaatc tggctgtcca
      421 gcagcagacc ctggaactga ttatggacca gaagaataag gggaccgcaa cccagttgga
      481 tgtcgatcgc gccgctgctc aggtatctgc gaccaaagca gagatgccca ccctgcagga
      541 acggcaatgg cagtccatgc atcggctcgc cgtcctgttg ggtgcaggac cggaagattt
      601 ggttgaagaa ttgtccccag ccgctcccat ccccgtaccg ccgcagcata ttggaatcgg
      661 gctgccgtcc gatctgcttc gccgccgtcc ggatatccgg cgggccgagc gccaactggc
      721 tgcttcaacc gcaagactcg gcgtggccgc agcggacctg tttccgcggt tttcgcttac
      781 cggttccgca ggtcttcaaa gttcaaatac caacaatcta ttggaaggcg acagcaacag
      841 atactttttc ggcccttccc tgaacctgcc gattttcaac ggcggacaat tgcacgccat
      901 ggtaaaagtc cgtaccgaac agcaggaaca atgtttgatg cagtatcagc aaacggtgct
      961 gacggctttg cgcgaggtgg aggatgcgat agtggctttt cgtttggagc agcagcggcg
     1021 gcagttcctc caggaaaccg tttcctcaaa tcaggaagcc gaggaactgg ctcagcagct
     1081 ctaccgcaat gggctgaccg attacttaac agttctggat gctcagcgtt ccctctataa
     1141 gtcccaggaa atgctggccg tcagtgaaaa gaaagtcaca atcaatttaa tcgttttata
     1201 taaggcatta ggaggcggct gggagccgga atcggctcca gtcgaaacgc cggaaacaat
     1261 gcccccggcc atggtatccc attcagtatc tcaaaaaacc gttttggatg taaccccata
     1321 gcaaagggac agattgaaaa acagcagcgc tgtttgaagg acgtaatctt atggcacatg
     1381 aagcaggaac acacagtcag cagacaccga atagagaagc agacacattt gtaaaaaata
     1441 cttctcaaaa gggaaaaatg gatatacccc cctatagtcg gccgttttat aaacgcccct
     1501 tctggatggc catcgtcatt ctgatgtcgg tattaattgt aactgctgtg gtatgttatt
     1561 ggctgcatag aaggcagttc gagagtaccg atgacgcctt tattgacggt gatattgttc
     1621 aggtatccgc taaggtgccg ggaattatcg aaagcgttta tgtggatgat aatcaggatg
     1681 tagcggcagg aacggtctta ctgcagattg accccaaaga ccttcaggcc cgcctgaatg
     1741 aggcccgcgc gtctttgatg gcggcggagg cgaaattggg cgaggcaaag gcccaggtcg
     1801 atgtggtcaa agccgcaacc gatgctttgc tgacgcaggc gaaagccggc gtggaacagg
     1861 caaaggccgc cgtcgaatcc agcatctcgc aattggcttc cgcccagtcc gatgtggtcg
     1921 cagcagaggc ggaagccgtc aagcgtcagg cggacctcaa acgcttcaag tccctcgacc
     1981 cgcgagtcgt ttcccagcag cagttggata gcgcccaggc ggcggcggat acggcggagg
     2041 cctatctggt ttcaactcgc aaacgggttt tatccgccga ggccgccgtg aaagaagcac
     2101 aagcgaagta tgctcaatcc ctcgctgttc tggcggcggc ggagaccggc cctcagcaag
     2161 tcgctgccgc cgtcgcacag atggagaacg cccggtccgg agtcgatgag gcaaaggcaa
     2221 aagtcgatgc cgccgaactg aacctctcct acaccaccat ccgggctccc gtcagcggac
     2281 gggtatccaa acggacagcc aggccggggc agtacctcca ggtaggccag aatgtcctcg
     2341 cattagtaca gccggatgtg tgggttaccg ccaattttaa agaaacccag ctcacctgca
     2401 tgtgccaggg gcagaatgtt gaaatcgaag tggatgctta cccgggacat gtatttcatg
     2461 gccatgtgga aagtattcag gccggaaccg gctcgcgatt cagtatgttg ccgccggaaa
     2521 atgcaaccgg caattacgtc aaagtcgttc agcgcgtccc tgtaaaaata cgattcgatc
     2581 agaaggatac acagcagcgg cttcttgccc ctggaatgtc cgttatcccc accgtacgcg
     2641 tgggacggga caaacagcag catccccagc cgattatgcc gccctctgag ccggccgtat
     2701 ctgaggcagc cgacctttca ccgaaagccg actgtccgtc cgaggttgcc caatgagccg
     2761 gccgtatcca tctgctcagc aaacggccga tcagccggca tcgacagccc atccatggct
     2821 cattgccgtt gctgtgatgt ctcccactgt tatggtggtg ctcgcaagca cggtggtcaa
     2881 tgtcgcattg ccgtatatag ccggcaccgt ctccgccggt acggaggagg taacctgggt
     2941 gattaccagt tatctggcgg cgaacgctgt aatcatgcct gccacgggct ggctaagccg
     3001 cctcttccgg cgtaaaagat tcgttatggg ctgtatatta acgttttctg ccggctccct
     3061 ggcctgcggg atggcacatt cgctcggctt tcttgtcgca gcacgtatca ttcagggact
     3121 cggcggggga gcgatgcagc ctgtcagcca ggcagtactc ctggagagtt ttccccctgc
     3181 caaacgagcg gttgccatgg cgatctacgg cgtcagcgtc atcgttgctc ccattgttgg
     3241 accgattctc ggcggatgga ttaccgataa ctactcctgg agatgggtct tttacctcaa
     3301 tcttcccatc ggattgattt ccctcctgat ggtttatctt tttgtggaag accctcccta
     3361 tattcggcag gggaccgcac gagttgacta tataggttta attctgctgg tcatttgggt
     3421 tgcaagtctg cttatcatgc tggacaaagg acagcaggaa gactggcttg attcccgttt
     3481 cattcgtact ctgctggtga tgtttatcct cggcctgaca gcatttattt tctgggaact
     3541 gcaagtccgg gagccggtcg tcaacttgca gattttgtgc aatccgactt ttacaatcgg
     3601 cacaaccatt aataccgtcg ccagcggagc ggttttattt tcctacacgg cctatctgcc
     3661 cctctttttg cagaccttgc ttggctatcc ggctttgcgg agcggcctga caatggcgcc
     3721 tcgcggattg ggcgcactta ttaccatgtt tctggtcgga cagctgatca gcgttgtcag
     3781 tccccgattg atgctggccg ccggattcct tacgctgggc tattccctct acgctgtcgg
     3841 caatctgaat ctggaaatat cgccccgcaa tgttgtgatt cccaatttct taagcgggac
     3901 cgccagcggc ctgatttttg ttcctttgac aacaatcacc atgggaaatc tgaggaggga
     3961 agaagtagcg aatgctacgg ggatttacaa tctgttgagg aatattgggt cagccatcgg
     4021 cattgccgtt atggccacgc ttatggcccg cggtgcacaa gtccatcaga caaatctggt
     4081 agctcacatg accccctatg acacggtgta ccagcaaaaa ttagaaagca tccagtcggc
     4141 cctgaccccg caaagcggcg catgcaccgc ggccgagcag gccgtggccg tgctgggcaa
     4201 agaattgctt cgccaatcca ccttgctctc ttatgtcgat aatttcaggt acctcgcatt
     4261 ggcctcggtg gcgtgcgtcc ctgcattgct gtttcttagc cgccagcgcc tcaaaagcgg
     4321 ctgaccgata tgcgaaggcg gattgacccc ataggaagcg gaacagttgg aggacgattc
     4381 aattatgagc aaaaaacctt tcatcgcgat tacaattgcc cgtcagatgg gtgccggcgg
     4441 ccttgtggtc ggacgcagcg tggctcgacg attgggatat gcctatcttg accgggccat
     4501 acttcgcatg gtggcccgcc agttgggaat aaccgataag gaactggaac attgggatga
     4561 acacgtttct accttctggg agcggctgag ccgagtgctg ctcattgggc cggccgaagc
     4621 aacctataca ggcgttcctc aagcagccgg aatccgtgat cggcagctgt tccttttgga
     4681 gtctcgcgtg attcgtgagg cggctttcag ccgtaatgtg gttgtcatcg ggcgggcggg
     4741 ctttgatgtg cttcgcgatc acccgggact tgccagcgtg tatttgcacg cgcctctgcc
     4801 cgctcgcatt gacattgtca tgcgtacgca tcaccttacg gagccggcaa aggccaggga
     4861 attgatagaa cgtacggatg ccgatcggat acgttttgtc cgggacatga caggccgact
     4921 cgcctatgat gccaactgct atcacctctc cattaacacc gaccgcaccg gccttgcatt
     4981 agcggaggag atgatagtca acctggtcga acatgtccaa cagatgcttg ccgggaactc
     5041 tcaataatcg ttcgcacgga ttcggattgt ttccctaccc atcaacccaa gcgagggctt
     5101 ttcttttggg cccgataata atatgcgaaa gatagtgtac gttatcctgg agcggctgct
     5161 catcgccgcg taaaactatg aaaaaagtca gtcaagttga gtaattatac gacagcgcga
     5221 cttatgctca agaagctgac agcataatgc cgataacttc tt
//
LOCUS       DAOTBO010000153         5226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_13253_length_5226_cov_5.582092, whole genome shotgun sequence.
ACCESSION   DAOTBO010000153 DAOTBO010000000
VERSION     DAOTBO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13253_length_5226_cov_5.582092"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLE88_12145"
     CDS             complement(<1..745)
                     /locus_tag="PLE88_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77257.1"
                     /translation="MGCLQPGQTSSLYSDALNRLADQLHYLNSSGDKTQDSTRYWFDT
                     RANLRREMEERKKRFDDKNEVRGRMADVVKKLAVGATFFDGTHIFTPHSDVPDDSALR
                     LVMLAPEQFYSREEPQLAVDGVLDYVRNNGTKPRYRGNRLIFVAPDHGALARLRDCIR
                     VALAWGSIVEDVAAMRLVLDNLQAEQARKELKSAEDVLPRVARECYRWLLCPSQDTPT
                     GKPSVETFPLNTSGAALGPEIERVCIDNEL"
     gene            839..1923
                     /locus_tag="PLE88_12150"
                     /pseudo
     CDS             839..1923
                     /locus_tag="PLE88_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611759.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2284..5103)
                     /locus_tag="PLE88_12155"
     CDS             complement(2284..5103)
                     /locus_tag="PLE88_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77258.1"
                     /translation="MDDDKVVVFGINYDSLSYVPPVEEKTTNESLEANSLESLDFGGE
                     SMMMSMGYQTEEPVWFDNPAECPDLDNDDFVNFVDYAIFAGNWLETGSELAGDFDEDG
                     TVDVNDLIHFCDYWLTPVDCPEYYSDPLPYQCSFEPSQGFVSTTDPNNPVSLDYQEGW
                     QVPSGIAELYETYYPSGQYLIFYNYVWVDPNTVITKDFISNETDDTYIRTSIVSNQGA
                     EILLMNDSNSIVAGLKFGSNNHYEVYDNGSYSSTSSTWQAEYWIDLMFVMNYSSNNYD
                     VYYYDWGSESYVQIGNDIDFDGSYSTINRVEYHTANLFSAVDKLSITDYSTSGGTFGE
                     SGTINITSPCACDTDDPLKGKVPVVGSVWWESLGDYVISCCPYDLDYTDKENWVEVAR
                     GDCVVDNDVLGHWNTDRIPNGYYYLRLDVCPDYDVWDTYTGSWVNTYGGSWIYKTLSI
                     GGTVVYDDLGYFPVVGELKSNTFYHEEEPDISVPWAGTFPFEFKRTFNNNRKFYTKPL
                     YNGWTHNSQIKLIEDTRYDWNLDTTAWPVPDYDADQLGMGYIWLEMPDGSRQLFTCEM
                     ENVQARYETITYKPYHNDNGEYIKRTSYVDLLDEKFDVYYTLYQRDGTELYFYSIDNS
                     LDIYSLDGGFVGWKVEMGISTMSDRFGNTLSYSWRKVSGKPVAVSSISGAGKQILFTF
                     DNDDRYTKAELKIGSTVYRTVEFDNEDIYDGTSIHSVTKKGKGVNEQGVYNGVTDKEY
                     VTKYEYDSGHNLRKVVNVNNSSQDETLIEVDYDGYGRVQTRKDYIESANYLETEFTYI
                     FDDPNVPGSGDNLVTIQSTDWQDNITVQNDDGAVIQQETITSDDSAVTNTDSDYEDLD
                     NPLKPTDTYEYFDGYERYTWNDYSNYGDLLEQQVYVEDSNYIATELTYHPDYAFETSQ
                     TNWQGLNKTGEKVQKLSIYGNANGT"
BASE COUNT         1410 a         1245 c         1240 g         1331 t
ORIGIN      
        1 caagttcgtt gtcaatgcac actcgttcga tctcaggacc caaggctgcc ccgctggtgt
       61 tgagagggaa ggtctccacg gatggtttgc ctgtgggtgt atcctgcgac ggacagagaa
      121 gccatctata gcattcccga gcgacacgtg gcaaaacatc ttccgcagac ttcagttctt
      181 ttctggcttg ctcagcctgg agattatcca gcaccagacg catcgctgca acatcttcta
      241 caatagatcc ccaagccagc gccacgcgaa tgcaatcgcg caatcgcgca agggctccgt
      301 gatcgggtgc cacgaagatc aagcgatttc cgcgataccg tggttttgta ccgttgttcc
      361 gcacataatc cagtacgccg tcgacggcca attgcggttc ttctcgcgaa taaaactgtt
      421 ctggtgccag cattactaaa cgcaatgcgc tatcatcggg tacatcgctg tggggagtga
      481 agatatgcgt accgtcaaag aatgttgcgc caacagcgag ctttttaacg acatccgcca
      541 tccgtcctcg aacttcattt ttatcatcga accgtttctt ccgctcttcc atctcccgtc
      601 gcagattggc acgggtatca aaccaatagc gggtcgagtc ctgtgtcttg tcgcctgacg
      661 agttcaggta gtggagttga tcggccaatc gattcagcgc atcggagtag agagaggatg
      721 tttggccggg ctggagacaa cccaactggc atgctccccg aaatttgatc caagttggtt
      781 gagaggatag ccgacagaag cagtctgaat aggactgatt ttgaaaggtt atcggacaat
      841 ggcaaaacaa tgcagcggaa cagagattgt tggaaaattg cgtcaggcgg atgtgcagtt
      901 ggcccagggc aagacggtgc cggaggtctg ccgcgagctg gggatcagcc agcagacgta
      961 ctatcgctgg cggaacaaat acggcgggat gagcccggac atgatcaagc agctgcgtgt
     1021 cctgcagaag gaaaacgctc ggctgcgtaa agtggtctcg gaccaggcga ttgacatcgc
     1081 gattctcaag gaggtggccc agggaaactt ctaagcccgg ctcagaagcg tcaggccgtg
     1141 gagacggcct gtgatgagct gggcgtttca cagcgaaagg cgtgtcaggc cctgcggcaa
     1201 tcgcggagta cccaacgtta cgatctgcgg ctgccgatca aggatgcggc aatcatcgca
     1261 gcgattcaaa agcagatgga aaagcgtccg cgattcggat accgccggat tacggtggaa
     1321 ctgcgcaagg acggctgggt ggttaatttc aagcgggtct accggatctg gcgcgatcac
     1381 gactggtctg tcccgaagaa aaagacccgt aaaaaacgcg gcggcgacgg cggctccgaa
     1441 aacgcctgcc ataagaaaaa gcccgagcat atcaaccacg tctggagcta tgattttatg
     1501 agccagacgc tggagaacgg ccgcaaagcg aggatcctgt gcgtgttgga tgaatatacg
     1561 cgggagtgcc tgacgattga tgtcggcaaa cgcatcacgg ctcaggatgt gatcgatgtg
     1621 ctgcgatatc tgttcctggt acggggtgag ccggcgttta tccgcagcga caacgggccg
     1681 gagtttacgg ccaaaaaggt caaaaagtgg ctcaaggcta tgggcgttaa aacgctctat
     1741 atcgagccgg gttcgccgtg ggaaaacggg tatatggaat cgttcaacgg gcacatgcgg
     1801 gatgagtgcc tggatcggga gatatttgtc gggatggaag agttgcgtta tatgtgtgaa
     1861 aagtaccgtc tggattacaa ccatcaccgg ccgcatcaga gtctggggta tttgacaccg
     1921 gctcaatatg cggccacggc tggaataccg ggggctctgc ccccgatccc ccggaattta
     1981 tcgctgaatg gatcagagca tcttattaaa gaagcaggcc gctcgaaagc ggccgatgct
     2041 ccgatcctcg cagcctgctc cggttgcatt cctgcagagc cgtatccttg ccgcgatagg
     2101 gaaagcatac gatgtaaaac caacgaacac aacatggcgg cagaaaacag attgaaatga
     2161 gccagaggca cttgtataat caagcatctt taatggcgtg ttcaggcgtt ctgaacacca
     2221 caggagaaga ctgcaaatgt cgaaaaccct ctcaccgaac ctggcacaaa gaaacggggc
     2281 aggtcatgtg ccattggcat ttccatagat acttaatttt tggacttttt caccggtctt
     2341 gttcaatccc tgccagtttg tttgggatgt ttcaaaagca taatccgggt gataggtcaa
     2401 ctctgttgca atatagttgc tatcctcgac ataaacctgc tgttcaagca agtcgccata
     2461 attgctataa tcattccatg tatagcgttc atagccgtca aaatactcgt aagtatctgt
     2521 tggcttaaga ggattgtcta aatcttcata atcactgtca gtattagtta cagcggaatc
     2581 atccgaagtg attgtttcct gctgaatcac ggctccatcg tcattctgaa cggtaatgtt
     2641 gtcctgccaa tcagtggact ggatggtcac taaattatca ccacttccgg gaacattggg
     2701 gtcatcaaag atataggtaa attcagtctc taaataatta gctgattcaa tataatcttt
     2761 tcgagtttgg actctcccgt agccatcata atcaacttca ataagcgttt catcctggct
     2821 ggaattgttt acattgacga cttttcggag attatgaccg ctatcgtatt catatttggt
     2881 aacgtattct ttgtcggtaa cgccattata cacaccctgt tcgttgacac cttttccttt
     2941 tttcgtaaca gagtgaatcg aagtaccgtc ataaatatct tcattgtcaa actccacagt
     3001 tcggtaaact gtcgagccaa ttttcaactc tgctttggtg tatcggtcgt cgttgtcaaa
     3061 agtaaatagt atctgctttc ccgccccgga tatactcgat acggcgacgg gtttgccgct
     3121 gacctttctc cagctataac taagggtatt accgaaacgg tcactcatgg tggaaatacc
     3181 catttcaact ttccagccga cgaatccgcc atccagcgaa tagatgtcaa gactgttgtc
     3241 gatgctgtaa aaatacagtt cagttccgtc ccgctggtac agggtgtaat aaacatcaaa
     3301 tttctcatcc aacaggtcaa catagctggt tcgtttaatg tactcaccat tatcattgtg
     3361 gtaaggtttg taggttatcg tttcatatct ggcctgaaca ttttccattt cacaggtaaa
     3421 caactgcctg ctgccgtccg gcatttccag ccagatatag cccattccta actgatccgc
     3481 atcataatca ggaaccggcc aagctgttgt atccaagttc cagtcatagc gggtatcttc
     3541 aatcagctta atttgactgt tatgtgtcca gccgttgtaa agcggtttgg tgtagaattt
     3601 acgattgttg ttaaatgttc gtttgaactc aaacggaaat gttcccgccc acggaacaga
     3661 tatatccggt tcttcttcat gataaaaagt attgcttttc aactcaccga caacagggaa
     3721 atatccaaga tcatcataga caaccgttcc gccgattgaa agagttttat aaatccacga
     3781 accgccataa gtatttaccc aactgcctgt gtaggtatcc caaacgtcat aatcaggaca
     3841 cacatctaac cgaagataat aatatccgtt ggggattcta tctgtgttcc aatgacctaa
     3901 aacatcatta tcaacaacac aatcacctct ggcaacttca acccaatttt ctttatccgt
     3961 gtagtcaagg tcataagggc aacaggaaat cacataatca ccaagcgact cccaccatac
     4021 agagccgacg acagggactt taccttttaa cggatcgtcc gtatcacaag cacagggcga
     4081 ggtgatattg attgtaccac tttcgccgaa tgtaccgccc gaagtggagt aatcggtaat
     4141 tgagagttta tcgacggcac tgaacaggtt agccgtatgg tattcaactc gattgatggt
     4201 gctgtaactg ccatcaaagt cgatgtcgtt accaatttgc acataggact cacttcccca
     4261 gtcgtaataa taaacatcgt agttgttgga gctataattc atgacaaaca tcagatcaat
     4321 ccaatattcc gcttgccatg tagaggatgt actggagtaa ctgccgttat catagacttc
     4381 ataatgattg ttgctgccga acttcaaacc tgcaacaata ctgttgctat cattcattag
     4441 aaggatttca gcaccctgat tggaaacgat gctggtcctg atatacgtgt cgtctgtttc
     4501 attgctgatg aagtctttgg taataaccgt attgggatcg acccacacat aattgtagaa
     4561 gataaggtat tgaccggaag gataataagt ctcatataat tcggcaatcc ccgacggaac
     4621 ctgccagcct tcttgataat caagtgatac gggattattg gggtcggtgg tactgacaaa
     4681 gccctgtgat ggctcaaatg aacactgata aggcagaggg tcggagtaat attccgggca
     4741 atcaaccggt gtcagccaat aatcacaaaa atgtatcagg tcattgacat cgaccgtacc
     4801 atcctcgtca aaatcgcccg ctaattcact tcctgtttcg agccagtttc cagcaaaaat
     4861 agcatagtca acgaaattca caaaatcgtc attatcgagg tctggacatt cggctggatt
     4921 atcgaaccag acaggttctt ctgtctgata acccatcgac atcatcatag attcaccgcc
     4981 aaaatccagt gattcaagag aattggcctc aagggattca ttggttgttt tctcttcaac
     5041 aggcggcaca tacgaaagcg agtcatagtt gataccaaag acaacaactt tgtcatcatc
     5101 catatcaaat aaaacaagct gaaaaacttc agtaccggcg ggaacagctt gattgtttat
     5161 cgtaagagcc tgtaaacttt cgcacttttt aggcgattaa ttttaatgga ttatcaacaa
     5221 aaacat
//
LOCUS       DAOTBO010000154         5215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_13285_length_5215_cov_5.464729, whole genome shotgun sequence.
ACCESSION   DAOTBO010000154 DAOTBO010000000
VERSION     DAOTBO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13285_length_5215_cov_5.464729"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLE88_12160"
     CDS             <1..843
                     /locus_tag="PLE88_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOM77259.1"
                     /translation="LLWIEDKTLSVFTSGDLKTWQRQGNIDGFFECPEFFELPVDDDP
                     NNRKWVVYGASGDYQIGRFNGTQFASESGIIKYEYGNCFYASQTFNHIPASDGRRIQM
                     GWGRIAMPGMPFNQMILFPVSLTLRTTDEGIRLFVNPVEEIASLHQQHWMWKQLTVKP
                     NDNPLSRITGELFHIQAVLTPDGADACRFVIRGVPVIYEAASERLTCQGQSAPLKRVD
                     GQIRLEILVDRMSIEIFANGGRVYMPVGVNLAGGPQTIAFSVEQGSTRIETLDIYTLK
                     SIWF"
     regulatory      1508..1630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1697..3040
                     /gene="thiC"
                     /locus_tag="PLE88_12165"
     CDS             1697..3040
                     /gene="thiC"
                     /locus_tag="PLE88_12165"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOM77260.1"
                     /translation="MSTQLQLARQGQISEEMRTVAQREGVDVHLIRSELAAGRLVIPA
                     NKIHLAMNLKPAAIGRAVSVKVNANIGCSSVRSSLDGEIEKMKAALEAGADAVMDLST
                     GGNLDEIRQKLIGICPVMLGTVPVYEVIVGRGVEDIDYSTILQIVEKQARQGVDFFTI
                     HAGLLREHLELIPRRVCGIVSRGGALMAKWMVYHNKQNPLYELFDDLCDIMREYDVCF
                     SLGDGLRPGCIADATDAAQIAELRVLGQLTQRAQEKGCQVMVEGPGHVPFHQIQKNME
                     MQQDICKGAPFYVLGPLVTDIAPGYDHITSAIGATAAAFYGASFICYVTPREHLGLPN
                     VSDVRTGVIAAKIAAHAADVARGFGNAAEQDCQLSKARANLDWEAHLSKAIDPPTARA
                     MYEEACRQSTMPSRGGDDYCTMCGREWCSMRINKELQTLYAGASGAGSSQWPNTD"
     gene            3022..4587
                     /locus_tag="PLE88_12170"
     CDS             3022..4587
                     /locus_tag="PLE88_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOM77261.1"
                     /translation="MAEYRLKPAVILTADRTLFADYRILFEGIFATMQTTKVPLWAMR
                     RFVAPRVRTSSAGRALVAPLGLRRVESALIQRLGLSPEEVVCTTPERLPSLIGPWVKA
                     VCFSSSDPLGMGMSNTTTTQFWSGELYTRYFSRQQLEYLRKAKNKYAFKVVGGGAGAW
                     QWLYYKDPAAQETLDCIYEGYFEDAGPELFKRILNGKSVDRHIRADAVGAAHICPIRG
                     ASGLGIIELSRGCGRGCRFCTIARKKMEHLPADIILSDLQTNVSAGIRSVVSGSEDFF
                     RYGSSGLKPDFEKLRALLIQMRQIQDLSFMQIDHANITSIAQLTDEQLREIRRLLSWQ
                     KPSRYLWVNMGAESASGALVAANCPGKIAPYRPEDWEGLLYETAERITRNGFFGVFSL
                     VLGLPGETPDDIARTRKLVRYLEHQNAVVFPVFYEPSNAGDISQGQRFTIETMQSEHL
                     ELYQACYEINFRKVPLLFWDNQRCGGVPWAKRLAMRLLGRGEIKQWRRAFRKTAERIR
                     SRTRAAGSVKNAG"
     gene            4577..>5215
                     /locus_tag="PLE88_12175"
     CDS             4577..>5215
                     /locus_tag="PLE88_12175"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOM77262.1"
                     /translation="MPDNQHIMLGQGNVCGGIPNSPQDRVKIRSACRLMVQQKNLVPP
                     MSLEGLKKNASAVLEQAGFGLEYLPFAAVIFNNELWRPVIERIPFSRRLLLIPQCLRH
                     IPDCPAQIDSLGLICRHCGRCMIDRILRRADELGYVSLVAEGSPVVMALIESGQVQAT
                     IGVSCLSVLERVFPYMEAGAVPGIAIPLLKDGCRHTYFDMDWLREAMEARDDQ"
BASE COUNT         1212 a         1263 c         1472 g         1268 t
ORIGIN      
        1 ctgctttgga tcgaggacaa aacactgtca gtttttacct ctggcgattt gaaaacatgg
       61 cagcggcagg gcaatatcga tggctttttc gaatgccctg aattttttga attgccggtg
      121 gatgacgatc caaacaatag gaaatgggtt gtctatggcg ccagcggcga ctatcagatc
      181 ggccggttca acggcacaca gtttgcgtcc gaaagcggaa tcatcaaata tgaatacgga
      241 aactgtttct atgcctctca aacattcaat cacataccgg cctctgacgg acggaggatt
      301 cagatgggct gggggcggat tgcaatgccg ggaatgccgt tcaatcagat gattttgttt
      361 ccggtttcat tgaccctgcg gacaaccgac gagggcattc gtctgtttgt caatcctgtt
      421 gaggaaatcg caagtctgca tcagcagcat tggatgtgga aacagctgac tgttaaacca
      481 aatgacaatc cgctcagccg gataaccggc gaattgttcc acatacaggc cgtcttaacg
      541 cccgatggcg ccgatgcctg tcggtttgtt atacgcggtg tacctgtcat ctatgaggcg
      601 gcctcggaac ggctgacctg tcagggccaa tcagcgcccc tgaagcgggt ggatgggcaa
      661 atccgtttag agatactggt ggatcggatg agtattgaga tttttgccaa cggcggcaga
      721 gtctatatgc ccgtcggcgt caatctggcc ggcggccccc aaacaatcgc tttttctgtc
      781 gaacagggca gcacccgcat cgaaacgctg gatatttaca cattaaaatc aatctggttt
      841 tagccgcttg ggaaattggt atcctttttc ccagtaaacc gatacacagt atcctgttcc
      901 aaagacagcg ggtatgcctg ttgaggtcgt acctatcgac aatatatatt tttttgcgta
      961 atttgatgtg ttgtcgatag ttgttgatta ctctcttatc ctctgtctct tatactctta
     1021 ctgtctatct gccagagctc ccatttcctc cgtcagcagc cattggtcct cgtcagttgc
     1081 caatgctgtc tatggtagac agttatccgc aagtaaaata tattagatac acaatacctg
     1141 caatctaatt gtcatatttt aaagtcgctt ttttcaaaac tgctgtttgg aggcgattca
     1201 tactggataa cttttttacc aagcggcttc tcagcaaaga ggatcaaatg cggcaacagg
     1261 tgacacgggt atcagataag attcgcaagg tcagtaagtt ttgcttttgt tccataccca
     1321 aagcggccaa atgtttccaa tccagccgaa agcagccaaa tgtgagatcc cagccggctt
     1381 tcacactggg gaaattgatt gtcctatctg aatcgtctga tgatgcgatt aatatccgtt
     1441 cctatttgtc ctgaaatatc ctgttaaaat gattttggca tttgtggact attccgttat
     1501 aatgacatcc gttcgctggg ggtggcctgt ctgatggtca ggcaggtctg agaaaaaccc
     1561 ttcgaacctg atcaggcggt atgctcggtg ggcagaccca acctgcgtag ggaagcgtta
     1621 atatgacgaa ggcgccgcac tatattgatc gcttccagta cacaagcaga agcgattttt
     1681 ttattgggag ttcaggatgt caacacaact gcagctggcc cgtcagggac agataagcga
     1741 ggaaatgagg accgtcgcac aacgtgaagg ggtcgatgtg catctgattc gttcagaatt
     1801 agccgccggc cggctggtta ttccggctaa taaaatacat ttggctatga atcttaagcc
     1861 ggcagctatc ggacgagcag tatcggttaa agtaaatgcc aatatcggct gcagcagcgt
     1921 gcgttcctca ctggatggcg aaattgaaaa gatgaaggcg gcacttgagg cgggagccga
     1981 tgcggtgatg gatttgagta ccggcggcaa tctggacgag attcgacaaa aacttatcgg
     2041 gatttgtcct gttatgcttg gcacggtacc cgtctatgag gtgatcgtcg ggcggggggt
     2101 tgaagacatt gattattcga ccattctgca aatcgtcgaa aaacaggccc ggcagggggt
     2161 ggactttttc accattcatg cgggtctgtt gcgcgaacat ttggaactga ttccgcgccg
     2221 cgtatgcggg attgtcagcc gcggcggggc actgatggcc aagtggatgg tttaccacaa
     2281 taagcaaaat cccctgtatg agttatttga cgacctgtgt gacataatgc gcgaatatga
     2341 tgtttgtttc tcgctgggag acggtcttcg gccgggctgc attgccgatg cgaccgatgc
     2401 ggcccagata gccgaactga gggtattggg ccagctgacg cagcgggcgc aggaaaaagg
     2461 ctgtcaggtc atggtcgaag ggccgggaca tgttccgttt catcagattc agaaaaatat
     2521 ggagatgcag caggatattt gcaagggcgc tccgttttat gtattggggc ctctggtgac
     2581 agatatcgcg ccgggatacg accatattac cagcgctatt ggggcaacgg cagcagcatt
     2641 ttatggggct tcctttatct gttatgtaac gccgcgggaa catttaggcc tgcccaatgt
     2701 gtcggatgtg cggacaggcg ttattgccgc aaaaattgct gcccatgctg ccgatgtggc
     2761 ccgaggattc ggcaatgcag ccgagcagga ctgccagctg agcaaggccc gtgccaatct
     2821 ggactgggaa gcacacttga gcaaagcaat agaccccccg acagcccgtg caatgtacga
     2881 ggaagcatgc cggcaaagca ccatgcccag ccgcggcggg gacgattatt gtaccatgtg
     2941 cggacgcgaa tggtgcagca tgcggatcaa caaggaactg caaacccttt atgcgggggc
     3001 ctcaggagct gggagcagcc aatggccgaa taccgattga aacctgcagt cattttgacg
     3061 gccgatcgga cgctttttgc ggactaccgc atcttgtttg aaggtatttt tgccacgatg
     3121 cagacgacga aggtgccgct gtgggcaatg cgtcgctttg ttgcgccgcg cgtgcgaacc
     3181 agcagtgccg gccgtgccct tgttgcaccg ctgggcctgc ggcgtgtgga gtcggctttg
     3241 atacagcgtc tgggattgtc gccggaagag gttgtctgca ctacacccga gcggctgcct
     3301 tctctgatag gcccctgggt caaggccgtc tgcttcagct ccagcgatcc tctgggaatg
     3361 ggcatgagca ataccacaac gactcaattc tggtcgggtg aactctatac gcggtatttt
     3421 tcacgacagc agctcgagta tctccgcaag gccaaaaata aatatgcgtt caaagttgtc
     3481 ggaggcggtg ccggagcttg gcaatggctg tattataagg atcctgctgc ccaggagact
     3541 ttggactgta tttatgaggg ctatttcgaa gatgccggcc ccgaactgtt caagcggatt
     3601 ctcaacggaa aatcggttga ccggcatatc cgagcggatg ccgtcggagc agctcacatt
     3661 tgtccgattc ggggcgcttc cgggctgggg attatagaac tgtcccgggg atgcggacgc
     3721 gggtgccgtt tttgcaccat tgcccggaaa aaaatggaac atctgccggc tgacattatt
     3781 ctcagcgacc tccaaaccaa cgtgtctgcc ggcatccgtt ctgttgtcag cggcagtgag
     3841 gatttcttcc gttacggttc ctccggcctg aagccggatt ttgaaaaact gcgtgctctt
     3901 ctgattcaga tgcggcagat tcaggatctg tcttttatgc agattgacca cgccaacatc
     3961 accagcattg ctcaattgac cgatgagcag cttcgggaga ttcgccgtct gctgtcgtgg
     4021 cagaagccca gccggtatct gtgggtcaat atgggtgccg aaagtgccag cggcgcattg
     4081 gtggccgcca attgtcccgg caaaattgcc ccttaccggc cggaagactg ggaaggactg
     4141 ctgtatgaaa cagctgagcg gataacgcga aacggatttt tcggggtatt cagcttggtg
     4201 ctgggactgc cgggcgaaac acccgatgat attgcacgta caaggaagct ggttcgatac
     4261 ttggaacatc agaatgccgt ggtctttccg gtgttttatg agccctccaa tgccggcgat
     4321 atctcgcagg gacagcggtt tacaatagag accatgcaga gcgaacatct ggagctgtat
     4381 caggcctgct atgaaatcaa cttccgaaaa gtgccgctgc tgttctggga taaccagcgc
     4441 tgcggcggag tgccttgggc caaacggctg gccatgcgac tgctgggcag gggtgaaata
     4501 aagcagtggc gaagggcgtt tagaaaaaca gccgaacgaa ttcgcagccg cacccgtgcg
     4561 gccgggagtg taaaaaatgc cggataatca gcatatcatg ttaggacagg ggaatgtatg
     4621 cggcggaata cccaacagcc cccaagaccg cgtaaaaatc cgctcggcct gccgtctgat
     4681 ggtgcagcaa aaaaatttag tcccgccgat gagtttagaa ggattgaaaa aaaatgcctc
     4741 ggctgttctg gagcaggctg gattcggtct ggaatacctg ccgtttgctg cggtgatttt
     4801 caataatgaa ctgtggcggc ctgtaatcga gcgcatccct ttttcgagac gtctgctgct
     4861 gattccccaa tgcctcaggc acattcctga ttgtcctgcc cagattgact cactgggtct
     4921 gatctgccga cattgcggcc ggtgtatgat tgacagaatt ctccgccggg ctgatgaact
     4981 ggggtatgtc tcattagttg ccgaaggatc gccggttgta atggccctga ttgaatcagg
     5041 acaggtgcag gcaaccatcg gcgtcagctg cttgtccgtt ctggagcgcg tgttccccta
     5101 tatggaggcc ggcgcggtgc cggggattgc catcccgctg ctcaaagacg gctgccgcca
     5161 tacatatttt gacatggatt ggctccgcga agcaatggaa gcaagagatg accag
//
LOCUS       DAOTBO010000155         5199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_13335_length_5199_cov_4.298795, whole genome shotgun sequence.
ACCESSION   DAOTBO010000155 DAOTBO010000000
VERSION     DAOTBO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13335_length_5199_cov_4.298795"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="PLE88_12180"
     CDS             complement(<1..1803)
                     /locus_tag="PLE88_12180"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77263.1"
                     /translation="MKTHRILFIAMILLPAASVVAELLLVEDFTDKTEAVTIDGKIPN
                     GVLGGAWDTEGESTGNITTRTSDGSMTLRVSAHSSGTLVRGAGIAGLTNPIDNTEAGI
                     LFFRFKVASGSRPLQDYLGIHTYTSSSYLNSTTCQGSAMNAGFSLFCAASSATFDIKT
                     IDNATVLKTGLARNQWYNVWIVADNARDTFDLYVNASSSPGRPVELPAESDRIGEGLA
                     FGMATASPLAGVTFGKPVPTLVSGQTEDVFIDDIYWDGDEGLTLTSKGARNPVPAHRA
                     VQVPLDQILSWDAPNDPNVAQIFGYDVYLDPNEAKVAAGLASVRVSTNQAVQFFDPVL
                     AYDTTYYWRVDSRIRLNDPNQTQLIVPSSVWRFTAESSLPAITRQPAYAVVKAGTAAV
                     FTITVESVFPPSYQWYKSSDPANDTFGDDILISGATGPTLTLSGVSTADEGFYYCKVT
                     NPRVAYSSAAGLAIQRPLAHWTLDGLVGGKYADTSGEGHHADPNGTPLFAAGVNPAKT
                     GNAVAINAGSGWANAGSWNPSQYSGQLSISLWVKWAGQWEAPYYQGLIGKRNAYTDDG
                     MMWQLEIGNTSPYNLTFKRYTTGTQTVAATGPLPV"
     gene            complement(2300..4555)
                     /locus_tag="PLE88_12185"
     CDS             complement(2300..4555)
                     /locus_tag="PLE88_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77264.1"
                     /translation="MRSIIKTICMITAVLLLGTAVQAELQFTENFDGRPIGNLIGATP
                     TAGNNGTVGVSNGTSSGNIQVQLQSGSNVCNIAGNSSGDRRGAGTYNMTDPIAVGEKG
                     VVFFRFLVDNTRSVDYYIGIHTRTTNINDSAIGSNPQNYLMAGFHLWSDGEPAGTMKM
                     VSTQHTATFATGLLRNQWYNCWIEADHAAETFSVYISPAEGPAGAATLPASSDKVVSD
                     RPFEVATTAPLAGMFVMGRTYSAEVAPWQRSTTQLGNIRVDEIYWDGSNGLASLTAQK
                     PDPANKAIDVAIEKVLSWEAPDDPKIAQVLGYDVYMDPNMAKVTNRDPSVLKSAAQTA
                     ASYDPTPDMAFVKTYYWVVDTIVTLVDDPNEPKVPRVHSGEVWSFTTTNPGPKITSDP
                     ADQLADLGGSASFTAGVSSPYVPATYQWYKSNDRSNSTPADDVLIGGATDLTYTVSNA
                     ALSDEKFYYLVASNVYNSVTYSAKSKPAALGVKRKVAHWTLDGLVGGKYEDISGEGHH
                     ADPNGSPVFYSGVNPALTNNAVWVNPDGGWATVGAWDPSEFTGKLTVSMWVKWNGQPA
                     TPRYQGLIGKRSVYATNMRWQLEIGNNAASLLTFKSNINGITGPILPVGEWEQVVVAY
                     DGTTATMYRNGTYAVSGPVTLNDGISSNLVIGGVGLDPAAVPPVATSILDGLLDDIQI
                     YNYALDPVTIAYSFTDVDPLGRKACVDPRDPILVAYDLNGDCEIGLGDLIDLANHWLE
                     GQLVPDVVARP"
     gene            complement(4604..>5199)
                     /locus_tag="PLE88_12190"
     CDS             complement(4604..>5199)
                     /locus_tag="PLE88_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77265.1"
                     /translation="KNNTPTVGTVTLYGYADALPGNRLVNIHTPWSNNNAYLDISNSD
                     AAGAYDRVAAGNAVNTAAEPYWIHWIFTKDSETGIQRIYRNGVRIGQATGLTRRLYGV
                     EHLQLGANRDTSGVAGQFFNGQIDDLRIYNYAVDEVEAAYMYHDATGAAVCINDADPV
                     VAKYDFNDNCKIDLPDLAELAANWLHCQQVPTCVARP"
BASE COUNT         1208 a         1467 c         1248 g         1276 t
ORIGIN      
        1 aaccggcagc ggtcccgtgg cggcgactgt ctgtgtgccc gtcgtatagc gcttaaaggt
       61 caggttgtag gggctggtgt tgccgatttc cagctgccac atcatcccgt catccgtata
      121 agcgttccgt ttgccgatga ggccctgata gtatggggct tcccactggc cggcccactt
      181 aacccacagc gaaatcgaca gctgcccgga atactgcgac gggttccatg agcccgcgtt
      241 ggcccagccg ctgccggcat tgattgcaac tgcattgccc gtttttgccg gattgactcc
      301 ggcggcaaac agcggcgtac cattcgggtc ggcgtgatgc ccttcgccgc ttgtgtcggc
      361 gtatttgccg cccaccagac catccagtgt ccagtgcgcc agcggtcgct gaatggctaa
      421 tcccgctgcg ctggaatacg ccacccgggg attcgttacc ttgcagtaat aaaagccctc
      481 atcggcagtc gaaacccccg acaatgtcaa tgttggcccc gtagctccgg aaatcaagat
      541 atcatcccca aaggtatcgt tagccggatc gctcgattta taccactggt acgaaggcgg
      601 gaaaacgctt tctaccgtaa tcgtaaatac tgcggctgtg cctgctttca caactgcata
      661 ggcgggctgc cgggtaattg ccggaagcga cgattcggcg gtaaatcgcc agacactgga
      721 aggcacaatc aactgggtct ggttcgggtc attaagcctg atgcgcgaat ccacacgcca
      781 atagtatgtc gtatcataag ccaaaacagg atcaaaaaat tgaaccgcct gatttgtact
      841 gacccgtacg gaagccaggc cggctgcaac cttcgcttca ttcggatcca aatacacatc
      901 atagccgaat atctgtgcca cattcgggtc attcggggca tcccatgata aaatctgatc
      961 caatggaacc tgcaccgccc gatgggcggg aacaggattc ctcgccccct tgctcgtcag
     1021 tgtcagcccc tcatcgccat cccagtaaat atcatcaata aaaacatctt cggtctgtcc
     1081 ggaaaccaat gtcggtacag gtttgccaaa tgtcacaccg gcaagcgggg aagcagttgc
     1141 cattccgaaa gcaagtccct ctccgattct gtcggactcc gccggcagct caaccggcct
     1201 gcccggactg ctggatgcat taacatataa atcaaaagta tccctcgcgt tgtccgcaac
     1261 aatccagaca ttgtaccact gattacgcgc aagtccggtc ttcaggactg tcgcattgtc
     1321 gatcgttttt atatcaaatg tcgcgctgga agccgcacag aataagctga atccggcgtt
     1381 catggcgcta ccttgacagg tcgtagagtt cagatagctg ctgctggtgt aggtatgtat
     1441 tcccagataa tcctggagag gccttgaacc ggaagcaacc ttaaagcgga aaaacaatat
     1501 accggcttcc gtattgtcaa tgggattggt aagccccgca atacccgcac cgcgaaccag
     1561 cgttccggaa ctgtgggcag atacccgcaa tgtcatgctc ccgtctgatg tacgggtagt
     1621 aatattgccg gtagattcac cctcggtatc ccacgcccct cccaatacgc cgttggggat
     1681 ctttccatca atggttactg cctctgtctt gtcggtaaag tcctctacca gcagcaattc
     1741 agcaacaacc gatgccgccg gcagtaaaat catcgcgatg aacagaattc gatgggtttt
     1801 catcacaatt tcctttcata tacagtccaa atcagcttta ccttgtccgg aactccagaa
     1861 tcgccgaaac catcgccgca cgatgcacac atcggttgcc ccaaccactg aaatatcccc
     1921 ctttgtggat tcataatatc ctgcccctgt ccttttccaa aaacatgcct ttgggcgata
     1981 atccctcaac agaacgacct tcccccctgt cttttatttt cccttttcct cacaattact
     2041 ttctggaaaa aaccgaatca gaaataattt cttttctcca tccctgttgt cctgtgtttc
     2101 tgggcaactt aaaggaatgc atttttttac taaaccacaa atccgggcat tttttgccgt
     2161 gctttttaac aaaacaccct gttcaggata tcgtttgccc gataccctga acagggcagc
     2221 attgcattgg cgtattccaa ttcctgccgt ccgggcagag atattaaacc gccaatttca
     2281 ttcacttctt tctggcgtct tagggacgag ccactacatc ggggactaat tgtccttcaa
     2341 gccagtgatt ggccaaatct atcagatctc caaggccgat ttcgcagtcg ccgttgaggt
     2401 cataagccac caaaatcgga tcacgaggat ctacgcaggc tttccgtccc agaggatcca
     2461 catccgtaaa ggaataagca atggtaaccg ggtcaagagc gtagttgtag atctggatat
     2521 catccagcaa tccatcgaga atcgatgttg ccacaggggg cacagccgcc ggatcaagtc
     2581 ctacgccgcc gataaccaag ttggaactga tcccgtcgtt aagggtaacc ggaccgctga
     2641 cggcataggt cccattgcgg tacattgtgg cggtcgttcc atcataggca acaacaacct
     2701 gttcccattc gcccaccggc agaatcggac cggtaatgcc gttgatgttg ctcttgaatg
     2761 tcaagagact ggcagcattg ttgccgattt ccagctgcca ccgcatattc gttgcataca
     2821 cactgcgttt gcctatcaaa ccctgatagc gcggagttgc cggctggcca ttccatttga
     2881 cccacatcga cactgtcagt ttgcctgtaa actccgaggg atcccatgcc cctacggttg
     2941 cccagccgcc atcgggattg acccataccg cattgtttgt gagagccgga ttgacaccgc
     3001 tgtaaaatac cggcgaaccg ttggggtcgg catgatgtcc ttccccgctg atatcttcat
     3061 acttgccgcc tactaatcca tccagtgtcc agtgcgcaac cttgcgtttt acgccgaggg
     3121 cagccggttt ggattttgcg ctataggtaa cgctgttata tacattagag gcaacaaggt
     3181 aataaaactt ctcatcagac agagcagcat tggaaacagt ataggtcaga tctgtagccc
     3241 cgccgatcag cacatcatca gccggcgtgc tgttgcttcg gtcattggat ttataccatt
     3301 gataggttgc cggcacataa gggctggaaa cacctgcggt aaatgaagca gatcctccca
     3361 agtcagccag ctgatcagct gggtcactgg taattttggg acccggattt gttgttgtga
     3421 acgaccacac ctcgccagaa tgaacgcggg gcactttggg ctcattgggg tcatcaacca
     3481 gcgtaacaat cgtatcgacg acccagtaat atgtcttgac aaatgccata tcgggtgtcg
     3541 gatcgtatga tgctgccgtt tgagcggcac ttttgaggac tgacggatcc cggttggtta
     3601 cttttgccat attcgggtcc atatatacat catagccaag cacttgagca atcttgggat
     3661 catcgggcgc ctcccacgaa agcacctttt caatagccac atcgattgct ttattggccg
     3721 gatccggctt ctgagctgtc aacgatgcta aaccgttgct gccgtcccaa tagatttcgt
     3781 ccacccggat attgcctaac tgggtggttg accgctgcca tggagcaacc tcagctgaat
     3841 aagtccggcc cataacaaac atcccggcca gaggagccgt tgtggcaacc tcaaacggcc
     3901 tgtccgatac gactttatca gaggaggccg gcagagtcgc tgctccggca ggtccttcag
     3961 ccgggctgat atacacactg aatgtctccg ctgcatggtc cgcttcaatc cagcagttat
     4021 accactggtt tcgcaagaga ccggttgcga aggtggccgt atgctgtgtt gacaccatct
     4081 tcatcgtgcc ggccggctca ccgtcgctcc acagatgaaa acccgccata agatagttct
     4141 gcggattgct tcctatagcc gagtcgttta tattggttgt ccgtgtatgg attccaatgt
     4201 agtaatccac cgaccgggtg ttgtcgacca aaaatcggaa aaacaccaca cctttttcac
     4261 cgactgcaat aggatcggtc atattatacg tgccggctcc gcggcggtcg ccgctggagt
     4321 ttcccgcgat gttgcacaca ttggagccgc tttgcagctg gacctgaata ttacccgatg
     4381 atgtaccgtt agaaacacct acggtaccat tgtttcctgc cgtgggtgtc gcaccgataa
     4441 gatttccgat cggacgccca tcaaaattct cggtaaactg caattctgcc tggactgcag
     4501 tgcccagaag caggaccgct gtgatcatgc atattgtctt gattatgctt ctcattactt
     4561 aaccctttca agtatatggt ttctcttcac cgtttcgacc gtctcaggga cgcgccacgc
     4621 acgtcggaac ctgctggcaa tgcagccagt tggccgccag ttccgcaagg tccggcaaat
     4681 caatcttgca gttgtcgtta aagtcatact tggcgacaac cggatctgca tcattgatgc
     4741 acacagccgc tcctgttgcg tcgtggtaca tataggccgc ttcaacttca tccacggcat
     4801 aattataaat ccgcaggtcg tcaatttgcc cgttgaagaa ctgcccggca acgcccgatg
     4861 tatcgcggtt ggcacccagc tggagatgtt ccacaccgta caaccggcgt gttaatcctg
     4921 ttgcctgccc aatccgcaca ccattgcgat aaatccgctg gattccggtt tcactgtctt
     4981 tggtaaaaat ccagtgaatc cagtatggct cggcagcagt attgactgca ttgcctgctg
     5041 ctacacggtc ataggcgccg gcagcatccg aattcgaaat atccagatac gcattgttgt
     5101 tgctccacgg agtatggata ttgaccagac ggttgcccgg cagtgcatcg gcatagccgt
     5161 aaagggttac tgtacccacc gtaggtgtat tatttttgg
//
LOCUS       DAOTBO010000156         5190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_13361_length_5190_cov_5.833496, whole genome shotgun sequence.
ACCESSION   DAOTBO010000156 DAOTBO010000000
VERSION     DAOTBO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13361_length_5190_cov_5.833496"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..754)
                     /locus_tag="PLE88_12195"
     CDS             complement(149..754)
                     /locus_tag="PLE88_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77266.1"
                     /translation="MKNTIIYSILLSIMLTCSSCQETEEKIYMPVEVWTGRNFHLTED
                     GLPVSIIWFRADRPYKGFCQAGEIKEVVERLLYPEIAGNIPELPLVLGTRNTLSLFYY
                     KGGKYVSRVAVIEFDIDENGFFLGPRGKTQTLAKLLQDKEESGLGRYAASGAPDEFNT
                     EERINQRIAWEAQYEREQEQKRVCSFNCVNGLNGFTSQLTL"
     gene            complement(765..1265)
                     /locus_tag="PLE88_12200"
     CDS             complement(765..1265)
                     /locus_tag="PLE88_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77267.1"
                     /translation="MNTNKKTLMGLLLIILSVGIAFFIGFKFHEQKTKIKIRDIRDIQ
                     ELKIATAYVRNPDGKTYQLSDPEKIKDALMNCDKTPVAFNVKVKRLQKLFMIFYEQGQ
                     NKILEVPFEIGYSYFKCEYGTSEELKNLLKTIPDPFAIPPWDTPHISDSNDFNAPSPE
                     NRSSEN"
     gene            complement(1281..4796)
                     /locus_tag="PLE88_12205"
     CDS             complement(1281..4796)
                     /locus_tag="PLE88_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77268.1"
                     /translation="MVSFAVATNGKKTVLSWNLFTIVLAFLVLTCFAHADIFLNINGD
                     DPAAWPVELSSPQSLEIRLLDSEPNNTSYDLTLTAIGGEFSHEGSSSSTLVIQTSDLE
                     SIGSIAFQLVDDPGLAVVSLTTNQPLAIATVFVPADTEVYQLIVFDMQEDNKVVVFGV
                     NYQSFSYISVEEEPTEQLFQMESLEKMEVDGGSTMMSMSGETELLAWDAELLGPLGSC
                     IIEPGTYRISSLPLQIEGHVVIPAGVRLIAPYDPNSIVIEVMPGGLLETGKAAFYSEE
                     DYPNVLPPVEIIPEDPNHYWFWHNSTGIYVHRGADSKTRIENLEIAGCMAGIVIDEAL
                     IHPVRYVITIACYDGIHLYAPVGIVDCEFWYNGSVWYWMDEYILAHFGEYMNGTLFED
                     ENISAYASAAVYVHLDGEAFPYPEISIERVVMDSADVGLYVRGINIDPNLPDPNVVAP
                     LVPQISVTNSTFSLTYFYGLYKTDTEATINVQYSAFAANSYYNTNVYISYIGYFVIPY
                     DPFYLLESSNRLYIDPWSHLINAGYGIATDGTGVCHDWPDTGQMDIGCHFALGLTGGF
                     GIPSSSADFNRDGIVDAYDLVLMEMCMGARSDPNIVRMDLDYDSWVNLGDFAFYACDY
                     GWASDPNLSLHADPNCARSDFNNNGFVNFADLEVLAEYWLTPVFDEYRICSLCNLHTG
                     TDPNDPNAPSGADLIDQRDMDAFMLDWEKTYAPDPDIVIEHSASMLSVAVENPVPAWR
                     ISAFLDDEPIGQWDNWELETPVFDLDMMRFGPGSHRLKIVRSIDYGLEITERIIADPN
                     SVGLYFADIPEYGDPNQTYEVSAYNPMNNQYSITFTDDLTGLIVDTVTASDEVMYVSV
                     PGSFLANGFASVTIKNVSSDKEIDRQIKKPFDPNDWAIRPRAVIICPDTHWWNGKDLF
                     VQRRPAILAAAQAFENQGIAYCVLYKKDVTPANLQFVLGNRSLRYVYWVGHGNRSVGD
                     PEIPRTSVNCWVNGKKVPAFSFTRNTISNAPALLTAAGNNYDHIGFDLNSITIDGRPM
                     REAGTKRLVVVDTCSSCVTHNLVNGEYDLAWVFGAYTYNPGWPLDEHMNYIGWARDVA
                     AGGGTIFNDDTTDRMVDFWNVLGSGNNLALAYERIWNNSATASASASLLPTFFGDDEL
                     NQYGKEDDTFKREGLAPKEQKIENR"
     gene            4913..5167
                     /locus_tag="PLE88_12210"
     CDS             4913..5167
                     /locus_tag="PLE88_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77269.1"
                     /translation="MHDRQDLSRSKDFMDQGWGDPQPAALRLHNNVYEKLSVDSKDLI
                     DIPHYWPQRKAPGLPRYQFIAREIRSGMMFLADAPIITAC"
BASE COUNT         1367 a         1251 c         1116 g         1456 t
ORIGIN      
        1 acagccgcca ccttatacaa cagaaatttt ggtattgata tcgggtaggg gtttagagct
       61 tcctgtctcg gcatcgtcct gacgctcagg ttgctctcca gcagagccct atccttcaga
      121 acgatatgga caaattaatg cgaaggggtt ataatgtcaa ttgtgaagtg aaaccgttaa
      181 ggccatttac acagttaaat gaacagaccc gtttttgttc ttgttcgcgt tcatattgag
      241 cttcccacgc tatgcgttgg ttgattcttt cttcagtatt aaattcgtca ggagcaccag
      301 aagcggcata tcttcccagt cctgattcct ccttatcctg caaaagtttg gctaaggtct
      361 gagtcttgcc gcggggacca agaaaaaaac cgttttcgtc aatatcaaat tcgatcaccg
      421 ctacacgcga aacatatttg ccacccttat aatagaacaa actcaatgtg ttccgagttc
      481 ccagcaccag cggcaattct ggtatattcc ctgctatttc cggatacagc aatcgttcga
      541 ccacttcttt gatttcgcca gcctgacaaa aacccttgta gggtcggtcc gccctgaacc
      601 agataatgct tactggaagc ccatcttcag tcagatggaa attgcggccg gtccacactt
      661 ctaccggcat gtagattttt tcttctgttt cttgacaaga agaacacgtc agcataattg
      721 aaagcaatat tgaatagata attgtgtttt tcatttgaat cttgctaatt ttcactagac
      781 cgattttccg gtgaaggggc gttgaagtca ttactatctg atatgtgcgg ggtgtcccaa
      841 ggtggtattg cgaagggatc aggaatagtc tttagcagat tcttcaattc ttcagatgtt
      901 ccgtattcgc atttgaagta actgtagccg atttcgaaag gaacttcgag tattttattt
      961 tgaccttgct catagaaaat catgaacaat ttttgaagtc gtttaacctt aacattaaat
     1021 gcaacaggtg ttttgtcgca attcattaga gcatctttaa ttttttcggg atcgctgagt
     1081 tggtaggttt ttccgtcagg attgcgaacg tatgcagtcg caatcttcaa ttcctgtata
     1141 tccctgatat ctctgatttt tattttggtt ttttgttcat ggaacttgaa tcctatgaaa
     1201 aaggcaattc ctacagatag tattatcagt aggagtccca ttagcgtttt tttgtttgtg
     1261 ttcataagtt gcggcctttt ttatcggttt tcaatttttt gttctttggg tgccaagcct
     1321 tctcttttga atgtgtcatc ttcctttcca tattggttaa gttcgtcgtc tccaaaaaaa
     1381 gtcggaagca gtgatgctga agcactggct gtagccgaat tattccagat tctttcataa
     1441 gctaaggcaa gattgtttcc tgagcccaat acgttccaga aatccaccat gcgatctgtt
     1501 gtgtcatcat taaagattgt tccaccgccg gcggctacgt ctcttgccca tcctatgtaa
     1561 ttcatgtgct cgtcaagcgg ccaaccagga ttgtaggtat atgctccaaa aacccaggcc
     1621 aaatcatatt ctccgttcac aaggttatgc gttacacaac ttgagcaagt gtctacaacc
     1681 accaatctct ttgttcccgc ttctctcatc ggcctgccgt cgattgtgat gctgttaaga
     1741 tcgaagccga tgtggtcgta gttgtttcct gctgccgtta acagagcagg ggcatttgat
     1801 attgtgttcc gggtgaaaga gaaggcagga acttttttac catttaccca gcagtttaca
     1861 ctggttcttg gtatctctgg atcgcctaca gaacggtttc catgtccaac ccagtaaaca
     1921 tatctgaggc ttctgttccc taaaacaaac tggagatttg ccggggttac atctttttta
     1981 tataagacgc aataagcaat cccctggttt tcgaacgcct gtgcggctgc aaggattgca
     2041 ggccgtcttt gtacgaagag atctttgccg ttccaccagt gtgtatccgg gcagatgatg
     2101 accgccctcg gtcttatggc ccaatcgttt ggatcgaacg gtttctttat ttgtctatct
     2161 atttccttgt cgctgctgac gtttttgatt gtaacggagg cgaagccatt tgcgagaaat
     2221 gaacccggaa cgcttacata catgacctca tcggatgctg ttacggtatc aacaatcagg
     2281 ccggtcaggt catctgtaaa tgttattgaa tattggttgt tcataggatt gtacgcgctg
     2341 acctcatacg tctgattcgg atcaccatat tcgggaatat ctgcgaagta caagccaact
     2401 gaattcgggt ctgcaattat ccgttctgtg atttccaagc cgtaatcgat actgcgaaca
     2461 attttaagcc gatgactgcc tggaccaaaa cgcatcatat ccaaatcaaa aactggagtc
     2521 tccaattccc aattgtccca ctgcccaata ggttcatcat cgaggaatgc cgaaatcctc
     2581 caagctggaa cagggttttc caccgccacc gacagcatcg aggccgagtg ctcaattaca
     2641 atatccgggt caggggcata cgtcttttcc caatcgagca taaaggcatc catatcgcgc
     2701 tggtcaatta aatctgcacc acttggagcg ttcggatcat tgggatctgt gcctgtatgc
     2761 aaattgcata gactgcatat gcggtattca tcaaacacgg gtgtcagcca gtattcggcc
     2821 aacacttcca agtccgcaaa attcacaaaa ccattattgt tgaagtccga acgtgcgcag
     2881 tttgggtcag cgtgcaaact caaattcggg tcgcttgccc agccgtagtc acaagcataa
     2941 aatgcaaagt cgcccaggtt tacccaactg tcataatcca agtccatccg aacgatattt
     3001 ggatcgcttc tggcccccat gcacatttcc attagcacca aatcgtacgc atctacaatt
     3061 ccatctctgt taaagtccgc cgaactggat gggatgccaa aaccacctgt cagccccagc
     3121 gcaaagtgac agccgatgtc catttgtcca gtatcgggcc aatcgtggca tacacctgta
     3181 ccgtcggtag caatgccgta tccagcatta atcaggtgcg accagggatc gatatacagg
     3241 cgattgctgc tttccagaag gtaaaatgga tcataaggaa ttacaaaata accaatgtac
     3301 gatatataaa cattagtgtt atagtaggaa ttggctgcaa aggccgaata ctgcacattg
     3361 atcgtcgctt ctgtgtcggt cttgtatagt ccgtagaaat atgttaggga gaaggtacta
     3421 tttgttacgc taatttgcgg aacaaggggt gcgaccacat tagggtcggg caaattcgga
     3481 tcaatattta ttcctctcac gtaaagcccc acatccgcgc tgtccatcac cactcgctca
     3541 attgaaattt caggatacgg aaatgcctca ccatcaagat gtacataaac tgcggcagac
     3601 gcataggctg aaatgttctc atcctcaaac aaagttccat tcatatactc accaaaatga
     3661 gccaatatgt actcgtccat ccagtaccat actgacccat tgtaccagaa ttcacaatcc
     3721 actattccta cgggagcata aagatgaata ccatcataac aggcaattgt aatgacatat
     3781 ctcacagggt gaatcaaagc ctcatcaatt acaatccctg ccatacagcc tgcgatttca
     3841 agattttcaa tccgggtctt cgagtcggct ccgcgatgga catagattcc ggtgctatta
     3901 tgccagaacc agtaatgatt tgggtcttca ggaatgattt ctaccggagg caaaacattg
     3961 gggtagtcct cttcgctata gaaagctgct ttgccggttt cgagcaatcc accgggcata
     4021 acttcgatta caatactgtt cggatcgtaa ggtgcaatca gcctcacacc agcgggtata
     4081 acaacgtgcc cttcaatctg caacggcagt gaagaaatgc ggtacgtccc cggctcaatg
     4141 atgcacgacc caagcggtcc caacagttcg gcatcccaag ccaagagttc ggtttccccg
     4201 ctcatcgaca tcatcgtcga gccgccgtcc acttccatct tttcaaggga ttccatttgg
     4261 aataattgct ctgtaggttc ttcttcaaca gagatataac tgaatgactg ataattgacc
     4321 ccaaaaacca ctactttgtt gtcctcctgc atatcaaaca cgataagctg gtaaacttct
     4381 gtatccgcag ggacaaacac agtagcgatt gcaagaggct gatttgtcgt cagactgaca
     4441 acggccagac ctggatcatc gactagctga aaagctatgc taccaatact ttcaaggtca
     4501 gaagtctgaa ttaccagagt agaggaactt gagccttcgt gtgaaaattc gccgccgatt
     4561 gcggtcagag tcaggtcgta gcttgtattg ttgggttcgg aatcaagcag tctgatttca
     4621 agagattgcg ggcttgataa ttccaccggc caagccgcag gatcatcccc gttaatgttt
     4681 aaaaagatat ctgcatgagc aaagcaagta agaacaagaa aagccaaaac aatggtaaaa
     4741 agattccaac tcaggacggt tttcttccca tttgtagcca ctgcaaaact caccataata
     4801 cctccatttt ctgatttaga ctatttaata caacaatctc ttcattgtca agcttttcta
     4861 aacttttggg tgggatggcc aaggaacatc gtatccccct caagaggatt cgttgcatga
     4921 cagacaggac ctatcgagaa gtaaagactt tatggaccag ggctgggggg acccccagcc
     4981 ggcggccttg cggctccaca ataacgttta tgaaaagctg tctgtggata gcaaggacct
     5041 gatcgatatc ccccattact ggccccagcg gaaggccccg ggcctgcctc gctaccagtt
     5101 tatcgcccgg gaaattcgca gcggcatgat gtttttggcc gatgccccga tcatcacggc
     5161 ctgctgaagg ttgcattcct gccgtgccgt
//
LOCUS       DAOTBO010000157         5184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_13381_length_5184_cov_4.907779, whole genome shotgun sequence.
ACCESSION   DAOTBO010000157 DAOTBO010000000
VERSION     DAOTBO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13381_length_5184_cov_4.907779"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLE88_12215"
     CDS             complement(<1..844)
                     /locus_tag="PLE88_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77270.1"
                     /translation="MKKIMYLLCVVLLAVFSDAETITVTDDPTGGGNSYGNCQGFAID
                     FDTTAPLAASSVTTGWNPPLVAGKSYALNSISIQYGGSVTVGNGSAVYLGIYTGGVNG
                     GGFLGVSKNTVNFETTATDEWVTWEFENINVIVDSAVGSGTGLLYFRYQTSPTDGAIN
                     GIEISTRRLNYDAAMTQVLASIIAYGTLQGARAPHYKADIGSKDTPFMPHEPVVLPEN
                     LDGSSGTLQPNNADVQVTFSFKAAMNAEQTAVNPDVVGHYIYLTKGTSDPNLYLLDYV
                     PHTDW"
     gene            complement(946..1632)
                     /locus_tag="PLE88_12220"
     CDS             complement(946..1632)
                     /locus_tag="PLE88_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77271.1"
                     /translation="MERRQKKNEAMKQVMAGLILALLVAGSASAEFVSATDGDGNLYG
                     NTEGFRIDFDATPASMADWNPDLVSGQTYKLDFVSIRNGSNSDATPLYLGVYSGFSAG
                     TWSGFLGVSDNAIDFAAVAAGAWAQYTFTGISVTVDAAVGSGSGMLYFLFQTGTAART
                     TAEVNRSIQRNNGYPGDPVTGDWLANIIAYGGVVDSRAPEYQAQLTIPEPATMVLLSL
                     GSLAIFRKRK"
     gene            complement(1822..3480)
                     /locus_tag="PLE88_12225"
     CDS             complement(1822..3480)
                     /locus_tag="PLE88_12225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM77272.1"
                     /translation="MKDTKKQLALAELIQKELDGCLTDQEFTCLQTILKENPEAVDYY
                     VKTICVISNISSNSAVLETVQGNSFLTESFQSGIWKALADAEKTAPAVAFKAAQMPRI
                     SVEKVRREKAVRKVSKSAIVSFLTAAAAVLFVILFARFAPPKGGYEVATLADGIGAEW
                     AAGTSVQKGSRFVTGSSAYMLKEGLAELLFDNAARLTIEAPAEFRILGDDRLRLEYGR
                     LYASVPPGAVGFTVNTPSARIIDLGTEFGVQSDFDGNTQLHVLKGKTVLMAGAADKTH
                     LEVSAGAAKKIAKATGEVTDIPCQSDYFVRTINSASNCVWRGQNRINLADIVGGGNGL
                     GTGTLDMMIDPASGKPSKESWGRRQASNDYHPVPSNPYIDGVFVPNGRSRQIVSSEGH
                     LFQDCPVTCGLTCDSLVNAQAMLDWLADSNAAAPETSVSVLLLHSNMGITFDLQAIRN
                     LLPGAKITRFQSKCGMRKWAVRSSASNADFWILVDGKLRHKQTQVKANSIFDIDIALS
                     DSDRFLTLVETDGGDPDERIFEDMILVPIDSDWGMFIEPVLILK"
     gene            complement(3482..4021)
                     /locus_tag="PLE88_12230"
     CDS             complement(3482..4021)
                     /locus_tag="PLE88_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77273.1"
                     /translation="MDIANLESKPTKESLFFQLFMSSQKNLYAYILASVHNFNDAEDI
                     MQETAAVMWQNFDKFDPDTNFLAWAISIARNMVRNYFSSRKKSRLQFDDSLMESIEQS
                     AIRNLERNSMRIEALKKCCEKLSESSRLLIQLRYEQGMTVKSIASRFGRPIAAMYKAL
                     ARLQDALLECTERIMAVEK"
     gene            complement(4364..>5184)
                     /locus_tag="PLE88_12235"
     CDS             complement(4364..>5184)
                     /locus_tag="PLE88_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM77274.1"
                     /translation="NRQTGPGRVMIKRLVNYYPLDEMNGNVTPDVVGDADMTLLTNGT
                     LLPSLSSDKAVGSGSLSLDPAAEPAGQYGQLPAGVLDYIDFTITAWVNWKGNSNGNWQ
                     RIFDFGNDTNQYVFLTPVGWNGVTRFVIKDGTEQIAGVDGNLPTNQWVHVAVTLSGDT
                     GRLYINGTLVATNTSMTFNPISFKPVLNYIGRSMWTGDAYFNGLIDDLKIYNYGLSVS
                     EVAQEYLAVAGGYVCNMEIYDMQNYDVNRDCKIDIADFVSFAARWLDEQRIYPQ"
BASE COUNT         1305 a         1434 c         1166 g         1279 t
ORIGIN      
        1 tccaatcggt gtgaggaaca taatccagca gatacaggtt gggatccgat gtcccttttg
       61 taagataaat ataatggccc acaacatcag gattgactgc cgtttgttcc gcattcatcg
      121 ctgctttgaa agaaaacgtc acttgaacat ctgcattatt cggctgcagt gtcccagatg
      181 aaccgtctaa attttccggc aggaccaccg gctcgtgagg cataaagggg gtgtccttgc
      241 tgccgatatc tgccttgtaa tgcggagccc gtgctccctg cagggttcca taagcaatta
      301 tactggcaag aacctgagtc atggctgcat catagttcag ccggcgcgta ctgatttcga
      361 tgccgttgat ggcgccatcg gtcgggcttg tctggtatcg gaaataaaga agccctgttc
      421 cggaaccaac agcggaatcc acaattacat tgatgttttc gaattcccaa gtaacccatt
      481 catcggtggc ggttgtttca aaattaactg tgtttttgga cacgcccaaa aatcctccgc
      541 cgttgacccc ccccgtatat attcccaggt aaacagcact gccgttaccg acagtgactg
      601 aaccgccgta ctgaatacta atagaattca gggcgtacga tttacctgca accaacggcg
      661 gattccagcc ggtggtaaca cttgaggcgg ccagcggcgc ggtggtgtca aaatcaatcg
      721 caaaaccttg gcagttgcca taactgttac cgccgcccgt aggatcatcg gtcaccgtga
      781 tggtttccgc atcggaaaaa acagctaata aaaccacaca taacaagtac attatttttt
      841 tcatactaaa cgccctccta aaaaaatttt ggttttttac caaaagcatt aatacaactc
      901 tccacccttg cttccaaact gcacttccta agaaatacac ttacgctatt tgcgttttct
      961 gaatattgcc agggaaccca aactcaacag aaccattgtc gccggctccg gaattgtaag
     1021 ctgggcctga tattccggcg cccgcgaatc gacaacaccg ccataagcta tgatgttggc
     1081 aagccagtct ccagtgacgg gatcaccggg ataaccgtta ttccgctgga ttgaacggtt
     1141 gacttccgcc gttgtacgcg ctgctgtacc ggtctggaac aggaaataca gcatgcccga
     1201 acccgaccca accgctgcat ccacggtaac gctgattccg gtgaatgtat attgagccca
     1261 cgcgccggca gcaacagcgg caaagtcgat ggcgttatcc gacacaccca gaaaaccgga
     1321 ccaggtacca gcagagaagc ccgaatacac tccaaggtat aaaggagttg catcactatt
     1381 cgaaccattc ctgatggaca caaagtccag tttgtaggtc tgcccggaaa ccagatccgg
     1441 attccagtcg gccatcgatg caggtgttgc atcgaagtca attcggaaac cctcggtgtt
     1501 tccatacaga ttgccgtctc catcggtagc cgacacaaat tccgccgacg ccgacccggc
     1561 aaccagcaga gccaaaataa gcccagccat tacctgtttc attgcttcat tctttttctg
     1621 tcttctttcc atctttcatc ctccaaaaat attgaatccc atcatcttac ctccaagagc
     1681 aaagaacagc tctttcctct tggttactca tacaatcccg tttaatcagt atcatccctg
     1741 tcccgcatat ggcacacatc tcacaccctt gatcccaact gttttcctga acaaatcgga
     1801 tttattcccc gcattccaaa ctcatttcag aatcagtact ggctcaataa acatgcccca
     1861 gtcactgtca atcggaacca gtatcatatc ttcaaaaatc cgctcgtcgg gatctccgcc
     1921 gtctgtttca accagcgtca gaaaccggtc gctgtcggac agcgcaatat caatatcaaa
     1981 aatcgagttt gccttgacct gtgtctgctt atgcctcagt tttccgtcta ccagaatcca
     2041 gaagtcggca ttgctggcgc tggaccttac agcccatttg cgcattccgc atttcgattg
     2101 aaaccgggta atcttagccc ccggcagcaa gttgcggatg gcctgcagat caaatgtaat
     2161 gcccatattg gaatgcagta ataagacaga cacggatgtt tcaggcgccg ccgcattgga
     2221 atcggcaagc caatcgagca tggcttgggc attgacaaga ctgtcgcagg ttaagccgca
     2281 ggtaaccggg caatcctgaa ataaatgtcc ttcggaactg acaatctgcc ggcttcggcc
     2341 gttcggaaca aacaccccgt caatataggg attggaaggc accggatgat aatcgttgga
     2401 tgcctgccgc cgtccccacg actctttgga gggcttgccg gaagccgggt caatcatcat
     2461 atcgagggta ccggttccca agccgttgcc cccgccgaca atatcagcca aattgattct
     2521 gttctgtcct cgccagacgc agtttgaagc cgagttaata gtacggacga aataatccga
     2581 ttggcaggga atatcggtta cctcaccggt cgccttggct atctttttgg ccgctccggc
     2641 actgacttcc agatgcgtct tgtcggctgc ccccgccatc agcacggtct tgcctttaag
     2701 aacatgcagc tgggtattgc cgtcgaaatc cgactgcacg ccgaattccg tgcccaaatc
     2761 aatgatgcgg gcagacgggg tgttgaccgt aaagccgaca gcccccgggg gtacagatgc
     2821 atacagccgt ccgtattcga gccgcagacg gtcatccccg agtatccgga actccgcagg
     2881 cgcttcaatg gttaatcggg ctgcattgtc aaaaagcagc tcggccagac cttccttgag
     2941 catatatgcc gacgagccgg ttacaaaccg cgaccctttc tgaacagatg tgcccgctgc
     3001 ccattcggcg ccgatgccgt cggccagagt ggccacctca tagccgcctt tgggcggtgc
     3061 aaaacgcgca aacaaaataa caaacagcac tgctgcggcc gctgtcagga aggaaacaat
     3121 tgcacttttg ctgacttttc gaactgcttt ttcgcgccgg actttttcca cgctaatccg
     3181 cggcatctgg gccgctttga atgcgacagc aggagccgtt ttttccgcat cggccagcgc
     3241 tttccaaatg ccgctctgga aagattcggt cagaaaagag ttgccctgta ccgtttccag
     3301 cacggccgaa ttcgatgaaa tattggatat cacacagatg gtttttacat agtaatccac
     3361 cgcctcggga ttttctttaa ggatagtctg caggcaggtg aactcctgat ctgtcaggca
     3421 gccgtccagc tccttttgaa tcaattccgc caatgccagt tgtttcttgg tgtctttcat
     3481 gtcacttctc aacagccatt atgcgttcgg tgcattccag cagggcatcc tgaagccgtg
     3541 ccagcgcttt atacatagct gcaatcggcc ggccgaagcg ggatgcaatc gacttgaccg
     3601 tcatcccttg ctcatagcgc agctgaatca gcaggcggct ggattccgac agcttttcac
     3661 agcatttttt taatgcctct atccgcatac tgttgcgttc caaattcctg atggcggact
     3721 gctcaatcga ttccatcaag ctgtcatcaa actgaagacg ggattttttc cggctgctga
     3781 aataattgcg aaccatattg cgggcaatcg aaatcgccca agccagaaaa ttggtatccg
     3841 ggtcaaattt gtcaaaattc tgccacatga cagccgccgt ttcctgcata atatcttcgg
     3901 catcattgaa attatggacc gaggccaaaa tataggcata caggtttttc tgcgagctca
     3961 tgaaaagctg gaaaaacaga ctctcctttg tcggcttact ttccagattc gctatatcca
     4021 ttttttccat ttatccatct ataatgacca gatacaggac cgataccgtc tccccaaagt
     4081 aataattcaa aaatccggaa ttggacatag atttgaaaaa aaatgtattc caaccgccta
     4141 tttcccctgt cagcaggccg cagccgttgg agtcgtgaag gggcttaacg ctttttcagc
     4201 taatgagata aatcaaataa gggagtatcc cgctatatct aaagatatta tatctccatt
     4261 aaatacaata atcttgttcc ccttgtttta aaacaaggtt tccggacgct ggttgggtgc
     4321 gtccggaaac ctttgacgtt accaaaagca ttccgtttgg atgctattgc gggtagatac
     4381 gctgttcatc gagccatcgg gcggcaaaac tgacaaagtc agcgatatca atcttgcagt
     4441 cgcggttgac atcatagttc tgcatatcat aaatttccat attgcacaca tacccgccgg
     4501 caacagccag atattcttgt gcaacttcgc tgacgcttag accataattg taaatcttca
     4561 aatcatcaat cagtccgttg aaataggcat cgccggtcca catactccgt ccgatataat
     4621 tcaaaacggg cttgaaactg attggattaa acgtcataga cgtatttgtc gcaacgagcg
     4681 ttccgttgat atacagccgg cctgtatcgc cgctgagcgt aacagccaca tgcacccact
     4741 gattggtggg cagatttccg tctaccccgg caatctgctc ggtcccatcc ttgatgacaa
     4801 accgggttac gccattccag ccgacagggg tcaaaaagac atattggttg gtgtcatttc
     4861 caaaatcaaa gatgcgctgc cagttgccgt tgctgttgcc cttccaattg acccatgccg
     4921 tgatggtaaa atctatataa tccagcacgc cggccggaag ctgaccgtac tgacccgccg
     4981 gttcagcagc cggatccaga ctcagactgc cgcttcccac cgctttatcc gacgacaagg
     5041 acggaagcag ggtgccgttg gtcaacagcg tcatatccgc atctccgaca acatccggcg
     5101 tcacattccc gttcatctca tccagcggat aataattcac cagacgcttt atcataaccc
     5161 ggccggggcc ggtctggcgg ttgg
//
LOCUS       DAOTBO010000158         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_14526_length_4846_cov_3.610937, whole genome shotgun sequence.
ACCESSION   DAOTBO010000158 DAOTBO010000000
VERSION     DAOTBO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14526_length_4846_cov_3.610937"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLE88_12240"
     CDS             <1..1910
                     /locus_tag="PLE88_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77275.1"
                     /translation="LIFAAKSTNQLGLPTSMNDTDFSDNALAITLGPVKFGVENEVYV
                     SFDAGTGKLLSSINHITRLYTITGSNASKNLDGTRSVSFLGSGAIIPGQMGGLVETGA
                     DQFPLLFWNNARNMVQTPGYNNQRGQVFENVVVLELLPHNPNPANGAVNVDPAVVTQL
                     QFNTAKDPGNLANQNPNVTGHYVTLFQIQNGEPNLVLPPVFEAFAPAGTDPISVNYTF
                     DLDDVVYWQVEEQISGCHKGDPNNIDGLVWYFKALPSSPVVTVSPGNVAVFLTEQASF
                     TCQFTSKAVPTAVEWYKTGSANPLDAADPDITITTIQSDGLYTSTLTIDNVEAADEGT
                     YYCCVENEVGQDISDSATLGVKRKVAHWTLDNNINGYNNGQYVDSSGEGHHAAPDGGP
                     VFSSNTPGILGGQSLNMTSTGNDRAAADSGNWATSLYTGETTVSAWVNWAGPDGGWQG
                     LVSNRRQVGMGYANYYIEIRQDNGNLQIGGIVGMGDLQVSPLPIGEWVHVAITAKAGE
                     VVIYLNGEAANRSTAGQAVPQNIVPLYIGALNRDTDSGALLSTFNGYLDDVQIFNYAL
                     TKTEVIDLYYAVMETPVCANLNNLDLRFDVAGGGDLGDQPDCQVNLADFAAFASSWFN
                     CGLYPAEECSQ"
     gene            2026..3222
                     /locus_tag="PLE88_12245"
     CDS             2026..3222
                     /locus_tag="PLE88_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77276.1"
                     /translation="MASDKRIKHRLILVMVLTLNVVIRAGEIVPGLDDSTALADYTAA
                     RAGVANDTAKITSGKITLAARFNPDPSQMTGGPIIVIETGGTTNGTGLYLGDGNLVLA
                     AKSNNQLGLPTSMNDTDFSDNALAITLGPIKPGVENEVYVSFDAGTGELLSSINRVTQ
                     LYTITGSNSSKNLDGNCSVSFLGSGAIIPGHMGGLVETGADQFPLLFWNNARNMVQTP
                     GYNNQRGQIFAASVAREILPYTILVSESEGTTTIQEGGASDDISIGITEDPADYPVTI
                     VAADLSEPIQVQAVPSQVVFDASNWQNVQSITLTAVDDSDKEPRFHTALLSFQVLVNP
                     ASPYFGFLLDDITVNIKENDCGVWGYDSVDMNQDCQIDLWDLSSLVQHWLECTFPEPG
                     CSDYRL"
     gene            3265..4536
                     /locus_tag="PLE88_12250"
     CDS             3265..4536
                     /locus_tag="PLE88_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77277.1"
                     /translation="MTRLIIVFGLACSVSAALVTENLIIHLDAGTLTGLNDGDAVVFW
                     ADSATEDSVDGSVTAVSGYGSPTYKTNQLGGKPVLRFVRQQEDLMVSSNWSLPNPSAG
                     LTIFVVCTGGSSGSVERVAQIGAAAGTASHLMAVDVSASASGCRYNNGYSLTLAGSNP
                     VASGLYHIGIRQMTQGGRHDSLYYAVNGLIPEPLTCNNPGNLIVFDAADNHISIGSGM
                     DPSGVWYPDFYNGDLAELLVYNAQLSSWQISQTAEYLSSKYALPFESAAVLVLESNGD
                     TTVREGGAADRIDVALTADPGMYPVTIQLRDFLNPNQVQVMPAELVFTSSNWQTPQSV
                     IVAAIDDDQMERATHDTQLELIVLADLSSPYYNISLSKIWVNIEDNDCGSLGFSPADY
                     NLDCVVDLEDFSIFVLEWIESSIPDPACQGF"
     gene            4582..>4846
                     /locus_tag="PLE88_12255"
     CDS             4582..>4846
                     /locus_tag="PLE88_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOM77278.1"
                     /translation="MNRRNFLKSTGLGFVALSGLRVCDLLSIGEKNRPNILFIFVDDM
                     GYADPSCYGNTKIQTPNIDRLARQGIQFTQFYANSPVCSPSRVA"
BASE COUNT         1145 a         1128 c         1325 g         1248 t
ORIGIN      
        1 atctcatttt tgcagccaag agtactaacc aattggggct gccgaccagc atgaacgata
       61 cggatttcag tgacaatgcc ctggccatca cgctgggacc ggtaaaattc ggtgtcgaaa
      121 atgaggttta tgtttcgttt gacgccggta cggggaaact gctcagcagc atcaatcaca
      181 tcacacggct gtacactatc accgggtcga atgcttccaa gaatcttgat gggacccgct
      241 cggtcagctt tcttggttcg ggcgccatta ttcccgggca gatgggaggt cttgtggaga
      301 ccggggccga tcagttccct ctgctgttct ggaacaatgc caggaacatg gttcaaacgc
      361 ccggctataa caatcagcga gggcaggtct ttgagaatgt cgtagtgctt gaactgctgc
      421 cccacaatcc aaacccggcc aatggtgccg tgaatgttga tccggcagta gtcacgcagt
      481 tgcagtttaa tactgccaaa gatccgggca atctcgccaa tcagaatccg aatgtgaccg
      541 gtcattatgt gacccttttt cagattcaga atggggaacc gaatctggtt ctgccgccgg
      601 tgtttgaagc ctttgcaccc gccgggacag atccgatttc cgtgaactat acatttgatt
      661 tagacgatgt ggtgtactgg caggtggaag agcaaatctc cggctgtcat aaaggagatc
      721 ctaataatat tgatggtctg gtttggtact ttaaagctct tccttcatcg ccggttgtga
      781 ctgtttctcc aggtaatgtt gctgtgttcc tcacagaaca ggcatctttt acgtgtcaat
      841 ttacgagcaa ggcggtacca accgctgtgg aatggtataa aactggcagt gccaatccgc
      901 tcgatgcagc agatcccgat attacgatta caacaatcca gagcgatggt ctctatacct
      961 cgactcttac tattgacaat gttgaagcag ccgatgaagg cacttactac tgctgtgtgg
     1021 aaaatgaggt cggacaggat atttctgact ctgcaacgct gggcgtgaaa cgcaaagtgg
     1081 ctcactggac gctggataac aatatcaatg gctacaacaa tggtcaatat gttgattcga
     1141 gcggggaagg ccatcatgcc gctccagatg gcgggccggt cttttcctca aacacaccag
     1201 gcattctcgg cggccaatcc cttaatatga cttctacggg caatgatcgt gccgcggctg
     1261 attcgggcaa ttgggcaacg tctttgtata ccggtgaaac gacggtgtcc gcctgggtaa
     1321 attgggccgg tcccgacggc ggctggcagg gtctggtcag caaccggcgc caggtcggca
     1381 tggggtatgc caattattac attgaaatcc ggcaggacaa cggaaatctc cagattggcg
     1441 gtattgtcgg tatgggcgat cttcaggtga gcccgcttcc gattggtgag tgggtccacg
     1501 tggctattac ggccaaagcc ggcgaagtgg tgatttactt gaatggcgag gcggccaacc
     1561 gaagcacagc gggccaggca gtgcctcaga atatcgttcc gctctacatt ggtgccttga
     1621 atcgggatac tgactcgggg gctctgctgt caacattcaa tggttatctg gatgacgtgc
     1681 agatcttcaa ttatgccctg accaagactg aagtgattga tttgtattac gcggtgatgg
     1741 aaacaccggt gtgtgcaaat ctgaataatc tggatcttcg ttttgacgtt gccggcggcg
     1801 gcgacttagg ggatcagccg gattgtcagg tgaacctggc cgattttgca gcgttcgcct
     1861 caagctggtt caattgcggt ttgtatcctg ccgaggaatg ctcgcaatga gtggttttta
     1921 atcaaaaggt ttgtttggat gaaaaggcct ctcaggcggc cgatttagat tagctggccg
     1981 cctgagaggg tttcaaagca taagatagga cctgttggaa gaaagatggc atcggacaag
     2041 cggatcaagc acagactgat tctggtgatg gtcctcacat taaatgttgt cattcgggcg
     2101 ggggagattg tcccgggact ggatgacagc acagccctgg cggactatac ggccgctcgt
     2161 gcgggtgtcg ccaacgatac cgccaaaatc accagtggca aaatcaccct ggcggctcgg
     2221 ttcaatccgg atcccagtca aatgacgggg ggaccgatta ttgtcatcga aaccggcggc
     2281 accaccaatg gtacaggtct gtatctgggg gacggcaacc tcgttttggc agctaagagc
     2341 aacaaccaat tggggctgcc gaccagcatg aatgatacgg atttcagcga taatgccttg
     2401 gctatcacgc tgggccctat caaacccggt gttgaaaacg aggtttatgt ttcgtttgat
     2461 gccgggaccg gagaactgct cagcagcatc aatcgcgtta cacagttgta caccatcacc
     2521 gggtcaaaca gttccaagaa ccttgacggc aactgttcgg tcagctttct cggttcgggt
     2581 gctattattc ccggccatat gggaggtctt gtggagaccg gagctgatca gtttcctctg
     2641 ctgttctgga acaatgccag gaacatggtt caaacgcccg gctataataa tcagcgggga
     2701 cagatttttg ccgcatcggt tgctcgtgaa atactgccgt acacgattct ggtatcggag
     2761 tcagagggca cgacgactat acaggaaggg ggggcttccg atgatatttc catcggcatt
     2821 accgaagatc ccgccgatta cccagtgacc attgtggctg ctgatttgtc agagccaatc
     2881 caagtacagg cagtgccctc ccaagttgtg ttcgatgcga gtaattggca gaatgtgcaa
     2941 tctattaccc ttaccgctgt ggatgacagc gacaaagaac cgagatttca caccgctttg
     3001 ttaagttttc aggttctggt gaatcccgca tcgccttatt tcggattttt gctcgatgac
     3061 ataacggtaa atattaaaga gaatgattgc ggagtctggg gatatgattc agtcgatatg
     3121 aatcaggatt gccagattga tttgtgggat ttgtccagtc tggttcagca ttggctggaa
     3181 tgcaccttcc cggaaccggg atgttcagat taccgattgt aatattcttc aggacggctt
     3241 aaaacgagag aacaagggat gaacttgaca agattaataa tcgtgtttgg actggcatgc
     3301 tccgtctctg ccgcattggt gacggagaac ctgatcattc atttggatgc cggcacgctc
     3361 acgggcttaa acgatggaga tgcggtggtc ttctgggctg attcggcaac ggaggattct
     3421 gtcgatggtt cggtaacggc tgtcagcggc tatgggagcc cgacctataa aaccaatcag
     3481 ctcggcggaa agccggtcct tcgttttgtc cgccagcagg aggatttgat ggtctcttcg
     3541 aactggagtc tgcccaatcc ttctgccggg cttacgattt ttgtggtttg tacaggaggc
     3601 agcagcggat ccgtagaacg ggtggctcaa ataggtgcgg cggcgggcac cgcttctcat
     3661 ctgatggctg tagatgtatc ggccagcgca tccggctgcc ggtataataa cggttattca
     3721 ctcacattgg ccggcagcaa ccccgttgct tcgggccttt atcatatcgg aatccgacag
     3781 atgactcagg gcggaagaca cgacagtctg tattatgcag tcaatggtct gattccggag
     3841 ccgctgacct gtaataatcc cggcaatctg attgtatttg atgcggccga caatcacatt
     3901 tccattggga gcggcatgga tccgagcggg gtctggtatc cggatttcta taacggcgac
     3961 ctagcggaac ttttagttta taatgcacaa ctctcaagtt ggcagatttc tcaaacagca
     4021 gaatacctaa gcagcaagta tgctcttcct ttcgaaagtg cggccgtgct ggtcctcgaa
     4081 agcaatgggg ataccacagt gcgggaaggc ggcgccgcgg accgtattga tgttgcgtta
     4141 actgcggatc ctggaatgta tccggttact atccagctgc gggatttttt aaatccaaat
     4201 caagttcagg tgatgcctgc tgaacttgtc tttacttcct caaattggca aactcctcaa
     4261 agcgtaattg ttgcggctat tgatgatgat cagatggaac gtgcaactca cgatactcaa
     4321 ctggaactga ttgtgctggc tgatctgtct tccccttatt acaatatttc gctgagtaag
     4381 atctgggtga atattgaaga taatgactgc ggatcgctgg gatttagtcc tgcagattac
     4441 aatttggatt gtgtggtgga tctggaggat ttttccatct ttgtcctgga atggatcgag
     4501 agcagtatcc cggatcctgc ctgtcaggga ttttgaatgg caatttcaga gaattgagtg
     4561 atagtaaaga gcggacgcag aatgaatcga cggaattttt taaaaagtac cggattaggt
     4621 ttcgttgctt tatcgggtct gcgtgtctgc gacctgctgt cgattggaga aaagaaccga
     4681 cccaacattc tttttatctt tgtagatgat atgggatacg ccgatcccag ctgttatggg
     4741 aatacgaaaa tccaaactcc caacattgat cgtctggccc ggcaggggat tcaatttact
     4801 caattctatg caaactcacc ggtctgttcc ccatcgcgtg ttgccg
//
LOCUS       DAOTBO010000159         4842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_14540_length_4842_cov_5.061625, whole genome shotgun sequence.
ACCESSION   DAOTBO010000159 DAOTBO010000000
VERSION     DAOTBO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14540_length_4842_cov_5.061625"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLE88_12260"
     CDS             <1..230
                     /locus_tag="PLE88_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77279.1"
                     /translation="VCRCSVTDSKPHIVIDIEADGFLYNMVRNIVGTLVEIGRGRWRP
                     ESMEHILAAKDRNAAGPIAPAAGLCLMKIYY"
     gene            291..1463
                     /locus_tag="PLE88_12265"
     CDS             291..1463
                     /locus_tag="PLE88_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM77280.1"
                     /translation="MKIVHIITRLILGGAQENTLITCKLLAQRGHEVTLITGPAEGPE
                     GALFEQTQTAGYRTVVLDDLQREINPIADIGAYFKLKKLLQQLDPDIVHTHSAKAGIL
                     GRYAGWALKNAAANKPAVVHTIHGLAFHPYQNPLLNRFYIAVEKAAATRTDAFITVAD
                     TMTEKCKAAGIGLDKPFTTAWSAIDQEAYLSPPKPDDIAAFRRQYHIPSDAVVLVKIA
                     RLFNLKGHDYVIESARRLAPNYPNCFWLFVGDGILAEPLKKQIHFAGVEDRFRFTGLL
                     TPQQIPLALHASDILVHCSLREGLARVLPQAMLCAKPVVSFDIDGAKEVVNQQTGFLV
                     EPENIDQLTKACEVLIADADLRQRLGQAGRISVRDKFAPHAMVDVIEAVYNTVLKG"
     gene            1541..2419
                     /gene="dapF"
                     /locus_tag="PLE88_12270"
     CDS             1541..2419
                     /gene="dapF"
                     /locus_tag="PLE88_12270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOM77281.1"
                     /translation="MMEFTKMHGLGNDYVYVNCFSQTVQNPAQLAVKVSDRHFGIGSD
                     GLILICPSTIADAKMRMFNADGSEAQMCGNGIRCVAKYVYDHHLARTHTEFSVPGLGT
                     FASSLRIETGRGVLTVGLDIDKNDKVRQVCVNMGQPILEASRIPVAGDAECVIDQELA
                     IEGQTLSMTCVSMGNPHAVFFCEDLQSIELAHIGPLIEHHELFPQRINVHFVKSVSPE
                     EFIMKTWERGSGITMACGTGACACAVAGVLTGRCQRRVRAHLPGGDLDLYWSEQDNCV
                     YMTGPAAEVYSGIWPD"
     gene            complement(2462..>4842)
                     /locus_tag="PLE88_12275"
     CDS             complement(2462..>4842)
                     /locus_tag="PLE88_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOM77282.1"
                     /translation="PASEIEDDTIYWMKKVADWQLTQSSWNSSVSWERAALHAGLLAC
                     YEVTKDEKYLDKSRQFAQKFNWQLASASHHADNNAVGQVFVELYLLDQPDPARYAHFR
                     NVSDSHIAVWPTFACDTAGGSETWWWCDALFMAPPGLVRLSRAANDPRYITFMHKMWL
                     DTQNCLYDTQEHLFFRDISYFYPARKNCNGDKVFWSRGNGWVLAGIARLLQYLPCDDP
                     GRPRYAVLLQEMAETLKTLQQPDGFWHSDLLGPNCYNNPETSGTGFFTFGIAWGINNG
                     LLDPSVYWPTVEAGWEALKTAVQPSGLLGWVQPVGADPRATTATTTDVFGVGAYLLAG
                     SEVYKYLLAQDPNSIELFESYADDASVRKVWLDGDRNGTAAHAALGDYGDNFMLLAYE
                     NNKPPYYAQTDCVFAAAKDFTATGTDYLSVLCRGLASNAPEVVYVRLEDLTGAAAVSV
                     MPDTAAVQTPVWLELAFRLSDFAGVDLTQVKQLSIGLGEPGRTSPAGSGVIRIDTLRL
                     AGRRCAAAEGDFNGDCTIDMEDFAVLAEQWQDEYALVIDPVEPDAENLLAFWSLDGDY
                     NDSSGRGCHAVPAGNPVFINPGHIGQSVYFDGASYLNCQNSADMTLHQGGTVSAWIKS
                     SGLTNPWASVVTKGIQSWRLIRNNTSSAISFHFNWSGGEYQANGSTPVLDNQWHHLLA
                     VYDGSSLRLYVDGQLDAAAAAPAAVNSRTDPVYIGSRVDKPASRSWTGQIDEVRIYDK
                     ALNQENLLWLAEKGPLVQISEARPADLMPDGVIDIQDLRIFALSWMERAVWP"
BASE COUNT         1138 a         1338 c         1273 g         1093 t
ORIGIN      
        1 ccgtctgcag atgttccgtc acagacagca agccgcatat cgttatcgat atcgaagccg
       61 acggttttct gtacaatatg gtgcgaaaca tcgtcggcac attggttgaa atcggcagag
      121 ggcgctggcg tccggaatcg atggagcaca ttcttgccgc caaagaccgc aatgcagcag
      181 gccctatcgc tccagcggct ggtttgtgtc tgatgaaaat atactattag cagcattcaa
      241 ttgaccggca tgttttgggc ggctaaaagg tgcagcaggg attttaagaa atgaagattg
      301 ttcacattat aacacggctg attctgggcg gcgctcagga aaacacgctg attacctgca
      361 agctgctggc tcagcgcggc catgaagtaa ccctgattac cggaccggca gagggccccg
      421 aaggagcgct gtttgagcag actcaaacgg ccggctaccg caccgttgtt ctcgacgacc
      481 tccagcggga aatcaatccg attgccgata tcggggctta tttcaaactc aaaaaactgc
      541 tgcagcagct ggatccggat attgttcata cccattcggc caaggcaggc attttgggac
      601 ggtatgccgg ctgggcttta aaaaacgcag cggcaaacaa acccgctgtt gtgcatacta
      661 ttcacggcct tgcctttcac ccctatcaga atcccctgct gaaccgcttt tacattgccg
      721 ttgaaaaagc agccgcaaca cgcacggatg ccttcatcac cgttgccgat acgatgaccg
      781 aaaaatgcaa agccgccggc attgggctgg ataaaccctt tacaacggct tggtcggcta
      841 ttgaccaaga ggcctacctg agccccccca agccggacga catcgccgct ttccgccggc
      901 aataccatat cccctcggat gcggtggtgc tggtaaaaat agcacgtctg ttcaacttga
      961 aggggcatga ttatgttatt gagtctgccc gccgcctcgc tccaaactat ccaaactgct
     1021 tctggctgtt tgtaggagac ggcatcttgg cagagccgct gaaaaagcag atccattttg
     1081 ccggtgtcga agaccgcttt cgcttcacgg gcctgctgac accccagcag attccgctgg
     1141 cgctgcatgc ttccgatatt ctggttcact gctcgctgcg tgaagggctg gcccgcgtgc
     1201 tgccgcaggc gatgctgtgt gccaaacctg tcgtcagctt cgacattgac ggcgccaagg
     1261 aagttgtcaa tcagcagacc ggctttctgg tcgagccgga aaatatcgat caactgacaa
     1321 aagcctgtga ggtattgatt gccgatgccg atcttcggca gcgtctcggt caggccggac
     1381 ggatttccgt ccgcgacaaa tttgccccgc atgcgatggt agatgtcatc gaagcagtgt
     1441 ataatactgt cctcaaaggg taaatagtcc gtttatggcc cttgtctttt ctttataatg
     1501 gatgacatgg ggcgacaaaa gtgctgcaat acaggagaaa atgatggaat ttacaaaaat
     1561 gcacggtctg ggcaatgact atgtgtatgt caactgcttc agtcaaacgg ttcaaaaccc
     1621 ggctcagtta gccgtcaagg tcagcgaccg gcactttgga atcggctcag acgggctgat
     1681 tctgatttgt ccttcgacta ttgcagatgc caaaatgcgg atgttcaatg ccgatgggtc
     1741 tgaagcccag atgtgcggca acggcatccg ttgtgtagcc aaatatgttt acgaccatca
     1801 tctggcgcgg acgcatacgg agttttccgt ccccggatta ggcacctttg catcttccct
     1861 gcggatagaa accggacggg gagtcctgac ggtaggcctt gatatcgaca aaaacgacaa
     1921 ggttcggcag gtttgcgtta atatgggcca gccgattctg gaagcgtccc ggattcctgt
     1981 tgccggggac gcagaatgcg ttatcgatca ggaattggcg attgagggcc agacgctatc
     2041 gatgacatgc gtatcgatgg gaaatccgca tgccgtcttc ttctgtgaag atctgcaaag
     2101 cattgaattg gcacatatcg gtccgttgat tgaacaccat gaactgtttc cccagcgtat
     2161 caatgtgcat tttgtcaaat ctgtcagccc cgaggagttt attatgaaaa catgggaaag
     2221 aggcagcggc attacaatgg cctgcggcac aggggcctgc gcttgcgccg tggctggggt
     2281 gctgacggga cgctgccagc gccgggttcg agctcacctg ccggggggcg atttagacct
     2341 ttactggagc gaacaggaca actgcgttta tatgactgga cctgcggcgg aggtctatag
     2401 cgggatttgg ccggactgat tcttctgccg gacatccgct atgctgcggg cagagggtct
     2461 atcagggcca gacagcacgt tccatccacg acagggcaaa gatccgaagg tcctgaatat
     2521 caatgactcc atcaggcatt aaatcagccg gccgggcttc tgaaatctga acaagcggtc
     2581 ccttttccgc cagccatagc agattttcct gattcagggc cttgtcatag atacgaacct
     2641 catcaatctg tccggtccag cttcgcgatg ccggcttgtc cacccggctg ccgatataga
     2701 ctggatcggt tcggctgttt acagcagcag gcgcagccgc cgccgcatcc aattggccgt
     2761 cgacatagag ccgaagactt gagccgtcat agaccgccag cagatgatgc cattggttgt
     2821 ccaaaaccgg cgtgctgcca ttggcctgat attccccgcc ggaccagtta aaatgaaacg
     2881 atatggcgct ggatgtattg ttgcgaatca atcgccagga ttgaatcccc ttggtcacta
     2941 ccgatgccca cggatttgtc aagccggagc ttttaatcca agcggataca gttccgccct
     3001 gatgcaatgt catatcagca ctgttttgac aattcagata gcttgcgccg tcgaaataaa
     3061 cagactggcc gatatgcccc ggattgataa acaccggatt gcctgccggc acagcatggc
     3121 agcctctgcc ggaagaatca ttgtaatccc catccaatga ccagaaggcc agcagatttt
     3181 ccgcatccgg ctcgacaggg tcgataacca gagcatactc atcctgccat tgttcggcca
     3241 gaactgcaaa atcttccata tcgattgtac agtcgccgtt gaaatccccc tcagcagcgg
     3301 cacaccgccg gcctgccaga cggagagtgt caatgcgaat tacccccgag ccggcgggac
     3361 tggttctgcc cggttcccca aggccgatgc tgagctgttt gacctgcgtc aaatcgaccc
     3421 ccgcaaaatc cgacagccga aaggccagct ccagccagac aggcgtctga actgccgctg
     3481 tatcgggcat cacactaacg gctgcagcac cagtcagatc ttccagacgc acatagacga
     3541 cttcaggcgc attcgaggca agaccccggc acaagacaga cagataatct gtcccggttg
     3601 cggtgaaatc cttggccgct gcaaacacgc agtccgtctg cgcatagtac ggcggcttgt
     3661 tattctcata ggccagcagc ataaagttgt ccccgtaatc cccaagcgct gcgtgtgcag
     3721 ccgtgccgtt gcggtctccg tcaagccata ctttccggac agaggcatcg tccgcatagg
     3781 attcaaataa ttcgatggaa ttgggatctt gcgcaaggag atatttatat acttcactgc
     3841 cggccagcaa ataggcgcct accccaaata catcggtggt agtcgctgtg gtcgctcttg
     3901 gatcagcccc gaccggctga acccagccca ataagccgct gggctgaacg gcggttttga
     3961 gtgcctccca gcctgcctca accgtcggcc aatagaccga tggatccagc aggccgttat
     4021 tgatgcccca tgcaatgccg aatgtaaaaa aacctgtccc gctggtttcg ggattattgt
     4081 agcaattggg tcccagcagg tcagaatgcc agaagccgtc gggctgctga agggttttga
     4141 gcgtttcggc catctcctgc agcaggactg catagcgagg gcggccggga tcatcgcaag
     4201 gtaaatactg aagcagccgt gcgattccgg ccagtaccca gccgtttccg cgcgaccaaa
     4261 acaccttatc gccgttgcag ttttttcggg ccggataaaa ataactaata tccctgaaaa
     4321 aaagatgttc ctgcgtatca tagagacagt tctgtgtatc cagccacatt ttatgcataa
     4381 acgttatgta gcgcggatca tttgccgccc tgctgagacg caccagcccc ggcggggcca
     4441 taaataaagc atcacaccac caccatgttt cactgccgcc ggctgtatcg caggcaaatg
     4501 tcggccagac ggcaatatgt gaatcgctga cgtttctgaa atgagcatac cgagctggat
     4561 ccggctggtc taacagatac agctccacaa agacttggcc gacggcattg ttatccgcat
     4621 gatgacttgc agaagccagc tgccaattga atttctgggc aaactggcgg cttttatcca
     4681 gatatttttc atctttcgtg acctcatagc aggccagaag ccctgcatgc agggccgccc
     4741 gctcccagct gacagaactg ttccagctgg attgcgtcag ctgccaatcc gcaacctttt
     4801 tcatccagta tatcgtatcg tcttctattt ccgaagccgg cc
//
LOCUS       DAOTBO010000160         4812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_14654_length_4812_cov_4.709481, whole genome shotgun sequence.
ACCESSION   DAOTBO010000160 DAOTBO010000000
VERSION     DAOTBO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14654_length_4812_cov_4.709481"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..718
                     /locus_tag="PLE88_12280"
     CDS             176..718
                     /locus_tag="PLE88_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77283.1"
                     /translation="MWANSDKNQLDRTKSFMRLVFANERRIYAYIYLLIPIQSEAEDI
                     FQDTLSVMWSKYDQFERERDFGAWGIGIASNLIKNYRRKRKNQSFFLEEDLENLLEKE
                     ARQSIKSLDARIEALRQCLSHLDPLDRKIIHMRYEEDIAVKAIAEKIRTTIKAIYVKL
                     ARSHDQLLRCIRRTLAEQGN"
     gene            721..2406
                     /locus_tag="PLE88_12285"
     CDS             721..2406
                     /locus_tag="PLE88_12285"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM77284.1"
                     /translation="MDDRKKSQWLCERILQFRDGQLPEDESADLKAVLRNDPQARRYY
                     IEIVVMHAIFQGRKSPLVKTNVYLPSEEVMDNELWEALAKEENSAEPIEVIRQQSESE
                     KAASAVVTVRETRVKNTPSKTPLYVLLASAAAIFLVILSIRYSPFKSGTAVAVLSDSL
                     EAVWSDSVLREKGAPLLTGPKPYILQQGYAQLVFNNHAQVTIQAPASFRLLDEDVLKL
                     DFGQVYAIVPPSAYGFQICTKESKIIDLGTEFGIEQTLSGDTEIHVLRGKVVLVSKAK
                     ERKINLNLSAGNACKLIAATGQTQQIECREGRFVRHINSDFRWAWKGQPLDLADLAGG
                     GSGFGGGDSRKGIDLSTGRMQPRANQGYDKPAAAGFRPVQDLPFIDGVFVPNGEFGPA
                     IVSSQGHQFDFPKTSGAYWSDITPNPYIAKKNPQTNEIEYVSTLLEVEGKPDEAIPPR
                     LLVHPNAGITFDLDKIRQAYPFFELKSFRAVCGLPQILDFRKRSEFWVLLDGQCVFHC
                     QSDYQNLSSRKVEIPIDPHARFLTLAATDGGDGTHYDWCVFAQPVLLLERSSL"
     gene            2436..4448
                     /locus_tag="PLE88_12290"
     CDS             2436..4448
                     /locus_tag="PLE88_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77285.1"
                     /translation="MKRIVLFLMGSAVLASCAYAALFEDFENYKLGLVGDVTKGKWVG
                     TDAVIVMNNLNKALDASDAVPVYASLGSFSIPDSDTQTTVFFRFYVATGSENSPIGVA
                     DEGDTSPDSYGDMDAYVQCISGNLTARNGSSSPTIISKISPGWYNVWLVINNNANIYD
                     VYVNQGTADAAEADKKATNFAFRTPSTTSIVSFANVYGSGHLFDDIYITPGLSLVNPP
                     RQVPPTPAAIEGFFMTNGSGLEYSTVINGHFTLPGSSSTGTIGVPGWYSNQTVWSSDS
                     GTRSGDSSDGDGWHFSEKVTDGPIWNVTQTKMVPGQSYTFFADAHGRWDCNRAYLSVV
                     IGEDPNTGLGDPNLSLATAEHVIGGNGIWYHDLFVSYTATQADAGKCIGLKLEGGSTT
                     KIDNSWIRYDNIRLYDGLAVVKHGPLNPVPADSSWDLAPGAFVLSWTPSNDPNCTGQR
                     LYYYIGTGGMFNLSAYNAVSPVSLSPSADFYNIGTLAFDQYVLWRVDTVIDGAAYTGP
                     TWFFVTQTADFPPIVDAGYSCITWLANLPQTLAGTVDDLGEGDVADADVVWSIVSAPS
                     GAAASVVKTSADPFQPTAVFTTDTAGTYTIQLTASDTGGSQGSQTSFDTLTVRVAADA
                     CAAQQMVQAGYNAADYDRNCVVDLADLSAFARQWLDDIRLTEAVPY"
     gene            4601..>4812
                     /locus_tag="PLE88_12295"
     CDS             4601..>4812
                     /locus_tag="PLE88_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77286.1"
                     /translation="MKRVLLVLALMFSTGAAVADFTANDNPLHLWNLNGSGTDNGSGL
                     IDIVTLANGARYDRWMNGIDFSADVTA"
BASE COUNT         1219 a         1115 c         1253 g         1225 t
ORIGIN      
        1 tatacatccg cgaaatatct gcttttattg gccaaacaag ggacatcttc tgtccgaaaa
       61 tattattttc gggcaagttt tgttgaagat tttggaaaaa taaagggaaa aacaaggtcc
      121 ttttgacaag tactttatag aggatgaata aaaataaatc gaacagggtt cacaaatgtg
      181 ggcgaattca gataaaaatc agctcgatag aaccaaatcc ttcatgcggc tggtatttgc
      241 aaacgaaagg cgaatttatg cctatatata tcttttaatc cccattcaat cagaagcaga
      301 agacattttc caggacacat tgtctgtaat gtggagcaag tatgaccagt tcgagcggga
      361 acgggatttt ggagcatggg gaatcggaat tgcttccaat ctgataaaaa attaccgaag
      421 gaaaaggaaa aaccaatctt tttttcttga agaggacctt gagaaccttt tggaaaaaga
      481 ggcccggcaa tccataaaat cactggatgc ccgtatcgag gccttgcgtc agtgtctttc
      541 tcatctcgat ccgcttgacc ggaaaatcat ccatatgagg tatgaagagg atattgcggt
      601 aaaagcaatt gctgaaaaga ttcgcaccac aatcaaggca atttatgtga aattggcccg
      661 ttcccacgat cagctgcttc gctgcattcg aagaacgtta gccgaacagg gaaactgagc
      721 atggacgacc ggaagaaatc tcaatggtta tgtgagcgaa tccttcaatt tcgggacggc
      781 caacttcccg aggacgagtc cgccgacctg aaagccgtat tgcgcaatga tccccaggca
      841 aggcggtatt acattgaaat tgttgttatg catgcgattt tccagggtcg taaatcccct
      901 cttgtgaaga cgaatgttta tctgccttca gaagaagtga tggataatga attgtgggaa
      961 gcattggcga aggaagagaa ttcagcagag ccgatagaag tgatcagaca gcaatcggaa
     1021 tcggaaaaag ccgcatctgc cgttgtcacc gttcgggaaa cccgcgtaaa aaatacgccg
     1081 agcaaaacac cgctttatgt tcttttggcc tctgctgcag ccatcttttt ggtcatactt
     1141 tccattcgtt attctccctt taaatccggc acagcagtgg ctgttttatc agacagttta
     1201 gaggctgtct ggagtgattc cgtcctccga gagaaagggg ctcctctttt gacagggccc
     1261 aaaccctata tccttcagca gggatatgct cagcttgtgt ttaacaatca tgcccaggtg
     1321 accattcagg cgccggcatc gtttcgcctg cttgatgagg acgtgctcaa actggatttc
     1381 ggacaggttt acgccatcgt tccgccttct gcctatggat ttcagatttg caccaaagaa
     1441 tcaaaaatta ttgatttagg cacagaattt gggattgaac agactctcag cggcgatacg
     1501 gaaattcatg tcctccgagg aaaagtggtc ctggtttcaa aagcaaaaga acgcaagatc
     1561 aatttgaatc tttcggcagg gaacgcctgc aaactgattg ccgccactgg acaaacacag
     1621 cagattgagt gccgcgaagg cagatttgta agacatatca attcagattt tcgatgggcc
     1681 tggaagggac agccgcttga cttagcggat ttggcgggcg gcggcagcgg cttcggaggc
     1741 ggagacagcc ggaaaggcat cgatttgtca accggcagaa tgcagccccg cgcaaaccag
     1801 ggatatgata agccggcggc ggctggattt cgaccggttc aggatttgcc cttcatagac
     1861 ggagtttttg tcccgaacgg cgagttcggc cccgcaatag tcagctcaca ggggcatcag
     1921 tttgactttc ccaaaaccag tggggcctat tggtctgata ttacccccaa tccgtacatc
     1981 gccaaaaaga atccccagac aaatgaaatc gagtatgtct ccacgctgct ggaagtggag
     2041 ggcaagccgg acgaagccat tccgcctcgg cttctggtgc atccgaatgc cggcattaca
     2101 tttgatctgg acaaaatccg gcaggcatac ccattctttg aactgaaatc tttccgggct
     2161 gtttgcggcc tgccgcagat cttggatttt cgaaaacggt cggaattctg ggtgctgctg
     2221 gatggacaat gtgttttcca ctgccagtcc gattatcaaa atctcagcag cagaaaagtc
     2281 gaaattccaa tcgacccgca tgctcgattc ttgaccttag ccgccaccga cggcggagat
     2341 ggaacacatt acgactggtg cgtgtttgcc cagccggttt tattgcttga aagaagttcc
     2401 ttgtaaacat ttactttttt cgggaggatt ttaagatgaa aagaatagtt ctgtttttaa
     2461 tgggaagtgc tgttttggcc tcatgtgcct atgcggcctt gtttgaagat tttgagaact
     2521 acaaattggg ccttgtggga gatgtgacaa agggcaagtg ggtagggact gacgcagtaa
     2581 ttgtaatgaa caatctcaat aaagcgttag atgcctccga cgcggtgcct gtctatgctt
     2641 cattggggag tttttctatt ccggattcag atacgcagac aacggtcttc ttccgctttt
     2701 atgtggccac cggcagtgaa aactctccaa tcggagttgc cgatgaggga gatacaagtc
     2761 cggacagcta tggggatatg gacgcttatg tccaatgcat cagcggcaat ctgaccgctc
     2821 gaaatggttc aagcagtcca accatcattt cgaaaatcag ccccggctgg tacaatgttt
     2881 ggctggtgat taacaacaat gccaacattt atgatgttta tgtgaatcag ggcaccgctg
     2941 atgcagcgga agcagacaag aaggcgacga actttgcctt ccgcacgcct tcgacaacct
     3001 ccatcgtatc atttgcgaat gtatatggct ccggtcattt atttgacgac atctatatta
     3061 cacccggcct gagtctggtc aatccgccaa gacaggtacc tccgaccccc gctgccattg
     3121 aaggattttt tatgacaaat gggtctggtc tggagtattc aacagtgatc aatggccatt
     3181 ttactcttcc gggttcttcc agcacgggca cgataggcgt tccgggttgg tacagcaatc
     3241 agacggtttg gagctcagac agcggtacac gcagcggcga cagctcggat ggtgatggtt
     3301 ggcatttcag cgaaaaagtc acggatgggc cgatttggaa tgtgacccag accaaaatgg
     3361 tgccgggaca aagttatacc ttttttgccg atgcgcacgg acgatgggat tgcaacaggg
     3421 cctatctgtc tgtagttatt ggcgaagatc ccaacactgg cttaggggat ccgaatctct
     3481 ccttggccac cgcagaacat gtgattggcg gcaatgggat ttggtatcac gatctttttg
     3541 taagttatac cgcaacgcag gcagatgccg gcaagtgcat cggcctcaaa ctggaaggag
     3601 gcagtactac caagatcgac aacagctgga ttcggtatga caatattcga ttgtatgatg
     3661 gattggctgt agtgaaacat ggccctctca atccagtacc ggcagacagc agttgggatc
     3721 tcgctccagg ggcgtttgtc ctgtcgtgga cgccaagcaa tgatccgaac tgtaccggtc
     3781 agcggctgta ttattacatc gggaccggag gaatgtttaa tctttctgct tataatgccg
     3841 tcagtcctgt ttctttatct ccatcagcgg atttctacaa catcggcaca ctcgcttttg
     3901 atcaatatgt cctctggcgc gtggatacag taatcgatgg agccgcatat acgggcccga
     3961 cgtggttttt cgtaacgcag acggcggact tccctcctat tgtcgatgcg ggctacagct
     4021 gcattacttg gctggccaat cttccgcaaa ctctcgccgg caccgtcgat gacctcggcg
     4081 agggagatgt cgctgatgcc gatgtggttt ggtcgattgt ttccgctcca tcaggcgcag
     4141 ctgccagtgt tgtcaaaaca tcggccgatc cgttccagcc gactgcggtt tttacaacgg
     4201 atacagcggg aacctataca attcagctga ctgcctccga tacaggaggt tcccagggct
     4261 cccagacttc gtttgacact ttgacagtcc gcgtagccgc agatgcctgt gccgctcagc
     4321 agatggttca ggcgggctac aatgcggctg attatgatcg gaattgtgtg gtggatctgg
     4381 cggatttgtc cgcctttgcc cgccagtggc tggatgatat ccgcttgacg gaagccgttc
     4441 cgtattgact ctctctttgg atagtttgaa tgaacaagag ttttcccctt ctgttccggg
     4501 gtcgggcata tacccggccc tggaacggga aaacaaagaa agccggcaga gatgatttgc
     4561 tggatgaaac cgtgcgaaag attttcagaa aggtagattt atgaaaaggg ttttgctggt
     4621 tcttgcgctt atgtttagta cgggggcggc ggttgccgac tttaccgcaa atgacaatcc
     4681 gctgcacttg tggaatctga acggcagcgg tacggacaat ggttcgggcc tgattgatat
     4741 cgttaccctg gccaatggag cacgatatga ccgctggatg aatgggatag atttctcggc
     4801 ggatgtcacg gc
//
LOCUS       DAOTBO010000161         4792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_14724_length_4792_cov_5.465484, whole genome shotgun sequence.
ACCESSION   DAOTBO010000161 DAOTBO010000000
VERSION     DAOTBO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14724_length_4792_cov_5.465484"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1356
                     /locus_tag="PLE88_12300"
     CDS             343..1356
                     /locus_tag="PLE88_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOM77287.1"
                     /translation="MELATFCWQLSTMLSGGLSITSAIQTIADEISNPYFEDVLKSIG
                     EKIEKGMSLTDCVKQYPKVFSKLCCAIIMAGETGGSLAVALERLAEHYENKDKLARKV
                     KGAMAYPAFVVGFIVLIVIVLMTLIIPRFTTMFKEFNGELPAFTRGFMAVYDAIAHNA
                     LYIVFGLVAAGIGLSLYARTRRGHLHFCKISLSLPIFGKILLMAFVASFCKTLSTLIG
                     AGVPLLDAFGILAEMTNNDLLRKGVLDTRDKMVEGVSISQSMEQIGFFPGVAVKMAQI
                     GENSGSLSSVMEKTAEYYEKKVDSLVSLMLGMLEPVLIITVGAIVLVVILAMYLPIFS
                     LSV"
     gene            1470..1856
                     /locus_tag="PLE88_12305"
     CDS             1470..1856
                     /locus_tag="PLE88_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM77288.1"
                     /translation="MKVKGFTLIELMVVILIVGILAAASVPMMRGRIDSAKWSEANAT
                     AGTIRSAVRVYFAEHGEAPTGVMNAANMSLLGFTSADLTGTYFVPGDYNITSVDANGN
                     ATITVTGSQTNAPSGSKTLSASGTWN"
     gene            2047..2520
                     /locus_tag="PLE88_12310"
     CDS             2047..2520
                     /locus_tag="PLE88_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM77289.1"
                     /translation="MQTAFTLIEVMLSILIVMVLVTGAMGYQYFSARDVKLAEVHTSA
                     ARLALLLLESWKGVQGDAGFDPVSTFAGQLDIQSAQEGPPAATNTGGALLTCLGFYQV
                     QLNGVCYYVTPAWQPPSDLEPMVLNVTIAWRRDYRPGPLTGDEPLVRYSAFLVQN"
     gene            2530..3063
                     /locus_tag="PLE88_12315"
     CDS             2530..3063
                     /locus_tag="PLE88_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM77290.1"
                     /translation="MKHYSVQADRCGFTLIELMTAIALVSIVILSIGFLLADGQRALA
                     RQRSRVFSDAAADGFAIQKVFDAVCRKASMRKAVIADEGRTLELYYWNAGSTASSPEN
                     YARFYQSDRDVFIQHGRLLPGTWQPDADAPVSTILLARQVDTIAFVAEGTSVQMFLTF
                     EDPDLMPVVCSSVRHND"
     gene            3076..4293
                     /locus_tag="PLE88_12320"
     CDS             3076..4293
                     /locus_tag="PLE88_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77291.1"
                     /translation="MGVQIIRKQRKGAILALMVMVILLLSMTSLALMKVGQEARLRTV
                     RNASQIAARFAADAGIEQVIYQMNKQLAAGAWTLDEIPVYTAQPMSGCTASYTVTFTS
                     GSLAGGYQITSEGRCGGQIRMVRATVGLTSPFAENYAVLTQGSMAFKNKSIVAGYNSA
                     DPLDTDVSVRIGTLSTGSGAVDIYNGSTIHADVYVGPEGDPQRVINQHGGASVAGEYF
                     VMPVSINLPVIEPPDYVASRGSLQVNSDLTLHAADSGKYTSINVKNGKTLIVGGDLTL
                     YVTGDIDLGNGAKIEVENNGSLKLYFKGSIDARNSAGISNKSRIPSKVKLFGTGTNQS
                     IDLKNDGALYGVVYAPEAQMTVHNKTDVYGSIIVHDFELKNGGNMYYDEALKNVSLDD
                     ECIRFAVTRWEDL"
     gene            4315..>4792
                     /locus_tag="PLE88_12325"
     CDS             4315..>4792
                     /locus_tag="PLE88_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOM77292.1"
                     /translation="MNHTAAHPVRKQAFTLIECLIVMLILMISIGGIMGFRYHAVLNA
                     ERAETHLLAARTAHVLIEAWKGHKGAADFDPTLPGFDSCFQIQPGSIFGTESAALTGS
                     EYRYLGTYRVNVEGRSFRADLVYQNDTGIDRLRTLYVVISWQDKQPRRLYLSTLTQT"
BASE COUNT         1165 a         1101 c         1330 g         1196 t
ORIGIN      
        1 gttttttttt ttttttgtta tcggacgctt cggaccgaag aaacgctcaa atttgtgaaa
       61 ttaagggctt gtttgagctt tcagccgaac cggccgttcc gaaatgacga tggaaggaaa
      121 atagagtgaa agacactatt ttgaaagctg gttataaaga cgtttgaata tgttgcatgg
      181 gattattcgg gcaattgcca ggagggagtc cggcaggccg ccagtcagga ggaactgctc
      241 agttttcttc gcagtcagga actgacgccg gcggttatta cggaagttgt cggatcggtt
      301 caggaaaaga cagcaccggt ccgctataag aaagtcaagt ctgtggaact ggcaaccttc
      361 tgctggcagc tgagcacaat gctgtccggc ggtctgtcaa tcaccagtgc tattcagacc
      421 atcgcagatg aaatttccaa tccgtatttt gaagatgttc ttaaaagcat cggcgaaaaa
      481 attgagaagg gaatgtctct gaccgattgt gtcaagcagt atccgaaggt tttcagcaag
      541 ctgtgctgtg cgattattat ggcaggcgaa acgggcggat cgctggcggt tgccctcgaa
      601 cgtcttgccg agcactatga aaacaaggac aaactggccc gcaaggtcaa aggagctatg
      661 gcatatccgg cttttgtcgt cggatttatt gtcttgatag tgattgtatt gatgactctg
      721 attattcccc ggtttacaac aatgttcaag gagttcaacg gggagcttcc agcttttacc
      781 cgcggcttta tggcggttta cgatgcaatt gctcacaatg ccctatacat tgttttcgga
      841 ttggtagcgg caggaattgg gctgtcgctg tatgcacgga cacgccgggg gcatttgcat
      901 ttctgcaaaa tcagcttgtc cctgccgatt ttcggcaaaa ttctgctgat ggcttttgtg
      961 gcctctttct gcaaaacgct cagcaccctt atcggggcgg gtgttcctct gctggatgcc
     1021 tttggcatac tggcggagat gaccaataac gatctgctgc gcaaaggggt actcgatacc
     1081 cgcgacaaga tggtcgaagg cgtcagtatt tcacaaagta tggagcagat cggtttcttc
     1141 cccggcgtgg ccgttaagat ggcccaaata ggagaaaaca gcggctcgct gtccagtgtg
     1201 atggaaaaaa ccgccgagta ttacgagaaa aaggtggatt cacttgtgtc gctgatgctg
     1261 gggatgctgg agccggttct gataataacg gtgggagcta ttgtgctggt ggttattttg
     1321 gctatgtatc ttccgatctt ttctctgtcg gtctaaagaa gaatgcagga aggattttac
     1381 ttaagatgaa ggtgttttcg aacgaaaaac aaaatagagt caacaggtag aatactatca
     1441 accttacatt taattgcagg agaacagaaa tgaaagtaaa agggtttaca ttgattgagc
     1501 tgatggtcgt aattctgatt gttggaattc tggcagctgc ttcggtcccg atgatgcggg
     1561 gccgcatcga ttcggccaag tggtctgaag ccaatgcaac cgccggaacg attcgctcgg
     1621 ctgttcgcgt gtattttgcc gaacacggcg aagctcccac cggtgttatg aatgcggcca
     1681 atatgagctt gctgggcttt acgtctgctg atttaacagg aacctatttt gttcccggcg
     1741 actacaacat caccagcgta gatgccaacg gcaatgcgac gattaccgtt acgggcagcc
     1801 aaacaaatgc tccaagcggt tccaagaccc tcagtgcttc tggaacatgg aactgacaga
     1861 cagcatattg ctagcgcacg gaggcggcaa gagatgcaac agaatctctt gccgcctctt
     1921 ttgtctgtaa gcagccggca cgggaggtat gggattggag gtgccggcaa atcagccgaa
     1981 tgaggaatgg gaatgatcta cagaggcaga atgccatacc cggcagcagc cagcaggcgg
     2041 attaggatgc agacagcatt tacgctgatc gaggtgatgc tgtcgatcct cattgtgatg
     2101 gtgctggtta cgggagccat ggggtatcag tatttcagcg cccgcgatgt aaaacttgcc
     2161 gaagtccaca cctccgcagc gcggcttgcg ctgctgctgc tggaaagctg gaagggtgtt
     2221 cagggcgacg cgggatttga tccagtcagc acttttgctg gacagcttga tattcaatcc
     2281 gctcaagaag gcccccctgc ggcgactaat acaggcggtg ccctgctgac gtgtctgggc
     2341 ttttatcagg ttcaattgaa tggagtgtgc tattatgtaa cgccggcttg gcagccacct
     2401 tcagaccttg aaccgatggt gctcaatgtt actattgcct ggcggcgcga ttacagaccg
     2461 gggccgctga caggcgatga acctcttgtc cggtattccg cttttcttgt gcagaactga
     2521 tcaaagacga tgaaacacta cagcgtacag gcagatcgat gcggatttac actgattgag
     2581 ctgatgacag ccatagcgct ggtttcgatt gtcattctca gcatcggttt tttgctggcc
     2641 gatgggcagc gggctttggc ccgtcagcgc agccgggtat tcagcgatgc agcggctgac
     2701 ggatttgcta ttcagaaggt atttgatgca gtctgccgca aagccagtat gcgtaaagcc
     2761 gttatagccg acgaaggccg aacgctggag ttgtattatt ggaatgccgg ctcgaccgct
     2821 tcctcgccgg aaaattatgc ccgtttttac caatcggaca gggatgtatt tatccagcac
     2881 ggcagattac tgcctgggac ctggcagccg gatgcggatg cacctgtttc aacaatcttg
     2941 ctggcacggc aggttgatac gatagcattt gttgcggaag gaacatccgt gcagatgttt
     3001 ctgacttttg aagatccgga tctgatgccg gttgtctgct cctcggtgcg gcacaatgat
     3061 tagcagggta gaacgatggg tgtacaaata atcagaaaac agcgaaaagg ggccatcttg
     3121 gcattgatgg ttatggttat tctgctgctg tcgatgacct cgctggcact catgaaggtg
     3181 gggcaggaag ctcggcttcg tactgttcgc aatgcttctc aaattgctgc tcgttttgct
     3241 gcagatgcgg gcatcgaaca ggtgatttac caaatgaaca aacagctggc agctggggca
     3301 tggacgctcg atgagattcc tgtctatacg gcacagccga tgtccggctg tacagccagc
     3361 tacacggtaa cttttacctc cggttcgctg gccggcggct accagattac gtcggaagga
     3421 cgctgcggcg ggcagatccg gatggttcgg gctacggttg gattaaccag tccttttgct
     3481 gaaaattatg ccgtcttgac gcaaggaagc atggccttta aaaataaatc gatcgtggca
     3541 ggatacaatt cagccgatcc gctggatacc gatgtatcgg tacgcatcgg cacgctcagc
     3601 acaggcagcg gagctgttga tatctacaac ggcagcacaa tccatgcgga tgtttatgtg
     3661 gggcccgagg gcgaccctca gcgggtcatt aaccagcacg gcggcgccag cgtggcgggg
     3721 gagtattttg ttatgcctgt cagcataaat ctgccggtaa ttgagccgcc tgattatgtt
     3781 gcctcacgcg ggtcgctaca ggtcaacagt gatctcacac tgcatgccgc cgacagcggg
     3841 aaatatacaa gcatcaatgt taaaaacggc aaaacgctga ttgtcggcgg agatttgacg
     3901 ctgtatgtaa ccggagatat cgatttggga aacggcgcca aaatagaagt ggaaaacaac
     3961 ggttctttaa agctctattt taaaggcagc atcgatgccc ggaactccgc aggcatcagc
     4021 aacaaaagcc gaatcccctc caaagtaaag ctgtttggca cagggaccaa ccagagcatc
     4081 gatttgaaaa atgacggagc attatatggt gttgtctatg ccccagaggc ccagatgacg
     4141 gttcataata aaacggatgt ctacggttct attatcgttc atgattttga gctgaaaaac
     4201 ggcggaaata tgtattatga tgaagccttg aaaaatgttt cgctggacga cgaatgcatc
     4261 cgttttgccg taacccgctg ggaggattta taattcttcc gaaaggaggt cgcaatgaat
     4321 catactgcag cgcatcctgt aagaaaacag gcatttactc tgattgaatg cctgattgta
     4381 atgctgattt tgatgatcag catcggcggc atcatgggct ttcgctacca tgcggttctg
     4441 aatgccgaac gggctgaaac gcaccttttg gccgcccgca cggcgcatgt gctgattgag
     4501 gcctggaaag gccataaggg agccgctgat tttgatccta ctttgccggg atttgattct
     4561 tgtttccaaa tacagccggg cagcattttc ggcacagaaa gcgctgcctt gaccggttcc
     4621 gaatatcgct atttgggtac ttaccgggtg aatgtcgaag gccgttcttt ccgggctgac
     4681 ctggtctatc aaaacgatac cggcattgac cggctgcgaa cactgtatgt agtgataagc
     4741 tggcaggaca agcagccccg acgcttatat ctgtccaccc tcacacaaac at
//
LOCUS       DAOTBO010000162         4779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_14767_length_4779_cov_4.064141, whole genome shotgun sequence.
ACCESSION   DAOTBO010000162 DAOTBO010000000
VERSION     DAOTBO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14767_length_4779_cov_4.064141"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="PLE88_12330"
     CDS             complement(<1..2008)
                     /locus_tag="PLE88_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77293.1"
                     /translation="MKNVILLLTTISMATISAHAVLLNHWIINEGSGFTVADAGTGGN
                     PGTLMEETAPAVSASEGPAWINDPIRGIVLSFDGTSDWILTNSQGVWGATPRTVALWF
                     NLARDNSDTNRHTLLEYGNTAAAGQYFRLLTENRRLRFEVISGNALALNAGDLSLNRW
                     YHVALVINDFNGDGQTRTTEVRFYLDGRYVPLTQSLNQIINTTFISDKYVRLGGGSQT
                     TASGSIPREPLAGMMDDVRIYNTALTDREIEALINSKAWNPSPANNSTNQGTLNNGKV
                     DVTFAWNTAVDCDNQSIPNPNIVKHYFYLNIGEPNFLHIAPVEISAGSPPQPTAYLGP
                     ISIDLDKTCYWRVDESILKNGIPSGPGDPNTITGMVWKFETQKSSLLITQQPVNILED
                     AGATVQFTVTAESVSAKNYQWYKSSDNANDTPADDIPVGTNSSVLTLSNIQLTDEAYY
                     YCVVSNVSEVPAVSNPAFLEIKRLTAWYAFEQNLADSAGGNGGTAIKADPYTPMNYTD
                     GIVGKAIVLNGIDEAVQIPRSIQNSFTIALWVKTTDIGGTGQWYVGKGLVDGEVSGQM
                     QDFGTSLSGTKFAFGIGNPDTTILSTSIINDGQWHYCVATRDQFTGNLSLYVDGLLEA
                     TGTGVLTPRNAASTLRIGSTNTGTLYLKGQIDEVKLYNYPLEGMA"
     gene            complement(2180..3829)
                     /locus_tag="PLE88_12335"
     CDS             complement(2180..3829)
                     /locus_tag="PLE88_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM77294.1"
                     /translation="MKPEQKKELYILLNALCEGIITDSQYQQLDEWLSNDPEACQIHV
                     DFSLIWADLKYFQSSLYSRQERIDALDRMECFESDNFADTEFWKALAEAEQKACPVEV
                     PRRPYPQNISPVKAPRIRSEKINKISLFTAVLTTTVMIFVIGVVPFLQIEHEPAACVL
                     ETMNAEWIDSALDYRQGDILNLNQSLYLKKGFAKIRMNDQSIVVIESPCKFTLEGEDQ
                     LLLTEGKITVQVPKEAIGFMVRTPSALVVDYGTELGVLVDQYAQTETHVRKGEVEMRV
                     GSNTRVFGQSIRLRANQAARVSGQTLTVIPPSIDKFTYEIPSPFEMYAKLLGAMAYIR
                     PIAYNSDSVRDIISNSELQIQTTPNLSIVPGPVPGNGKQSLGFRIQGALPSIQIGNLR
                     SLPQHSEGFNSIGFWIYFESIRQQFIYADYDKGKKNRLMIMNSDGHLEHSAYSAQGKW
                     RIVSSSEPLKPGTWYFVMITRTWETKNEKIMYINGKPAARTVIDAMNDTISLFDFTQI
                     GGDSNQIQGMDGAIAEFLIFPRALTEQEIQHLYQSAVVSGK"
     gene            complement(3826..4383)
                     /locus_tag="PLE88_12340"
     CDS             complement(3826..4383)
                     /locus_tag="PLE88_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77295.1"
                     /translation="MIEKDRNHDELTADRGELFMKLYTDAQRRLYAYVISLVPDESSV
                     EDIIQETATYMWRHFNEFEIGTNFPAWAFSIAKNRILAHIKNQQKTRRHFSTQTLQEI
                     EKTVAARLESMDDRLWALRKCIEKLSTNDRQLLILRYEIGASLKSVSERLRQSVNTLY
                     NRLYRIRILLMHCIEKTIGKENLPG"
BASE COUNT         1217 a         1114 c         1014 g         1434 t
ORIGIN      
        1 cagccatccc ttcaagagga tagttataca atttgacttc gtcaatttgt cccttcaaat
       61 acaatgtgcc ggtgttggtc gaaccgattc gcagtgttga cgcagcattg cgaggcgtta
      121 atacgcccgt tcctgttgcc tcgagcaggc catccacata aagactcaaa tttccggtaa
      181 attgatctcg cgttgctacg cagtaatgcc attgcccgtc attgattata ctggttgata
      241 aaatcgtagt gtctgggttg cctataccga atgcaaactt ggttccgctt agtgatgttc
      301 cgaagtcctg catctggccg cttacttctc catccactaa accttttcct acataccatt
      361 gaccggttcc gccgatatca gtagttttga cccagagtgc gattgtaaag ctgttttgga
      421 tggagcgtgg aatttggacc gcttcgtcta tgccgttaag cactatggcc tttccaacta
      481 tgccatccgt atagttcatt ggggtataag ggtcagcttt aatagcggta ccaccattgc
      541 cgcctgccga atcagcaagg ttttgctcaa aagcatacca tgctgtgaga cgcttaattt
      601 caagaaaagc cggattagaa acagccggaa cttcgcttac attgctgaca acacaataat
      661 aatatgcttc atcggtcaac tgaatgttgc tcaatgtcag gacgcttgag ttagttccta
      721 ccggtatatc atccgctggc gtatcattag cattatcaga tgatttatac cactgataat
      781 ttttggcaga cacactctct gcggttaccg taaactgaac tgttgctccc gcgtcttcaa
      841 gaatattgac tggctgctgg gtaatcagaa gtgatgattt ttgtgtttca aatttccaga
      901 ccattcctgt aatggtattg ggatcaccgg gacccgaagg tatgccgttt ttaagtatgc
      961 tttcatctac gcgccagtaa caggttttgt caaggtcaat tgaaatcggc ccaagatatg
     1021 ccgtcggctg gggcggcgaa cccgccgaga tttctacagg ggcaatatgc agaaagttag
     1081 gttcgccgat gttcaagtaa aaataatgtt ttacgatatt cggattggga atggattgat
     1141 tgtcgcaatc gacagccgta ttccatgcaa atgttacatc cactttaccg ttgttgaggg
     1201 taccctgatt cgtggaattg ttggccggag agggattcca tgccttgctg ttgattaaag
     1261 cttcgatttc ccggtctgtt aatgccgtat tataaatccg gacatcatcc atcatgccgg
     1321 ccaaaggttc gcggggaatg cttccactcg ccgtcgtttg agagcctccg cctaatcgaa
     1381 catatttgtc ggatataaaa gttgtattaa tgatctggtt tagagactgg gttaaaggca
     1441 cataccggcc gtccagatag aatcttactt ccgttgtgcg tgtttgtccg tcgccgttga
     1501 aatcgttgat aaccagtgct acatgatacc atctatttaa actcaaatca ccggcattca
     1561 atgctaaagc attgccgctg attacttcaa atcgcaacct tcggttctcc gtaagcaatc
     1621 ggaaatactg tcctgctgcc gcggtgtttc cgtattcaag cagggtgtgg cgattggtat
     1681 cgctattatc ccgtgccaaa ttgaaccaca gagccacggt gcggggggta gctccccaaa
     1741 caccctggga attggttaaa atccaatccg atgtgccgtc gaaactcaga actataccgc
     1801 gaatcgggtc attaatccat gcggggcctt cggatgccga cactgcgggt gcagtctcct
     1861 ccatcaaggt acccggattg cctcccgtac ctgcatctgc aaccgtgaat ccgctgcctt
     1921 catttatgat ccagtgattt aacaacaccg catgtgcaga tattgttgcc atgctgatcg
     1981 tggttaataa caaaattaca tttttcattg cattccctca ctttccttaa aaacaattag
     2041 tcttttttgt ttcgcttcaa aatcagttcc accaggacca ttttcaccgc ttctgcatcc
     2101 cctcgctctg ctgctcatca ccgcctaaaa gccatgaaaa aagtccggtc caattgaata
     2161 accttatgta ccttatctgt tattttccgg aaaccacggc tgattgatac agatgctgaa
     2221 tttcctgctc ggtaagagct ctggggaata tcagaaattc tgctatagct ccatccattc
     2281 cctggatttg attagaatca cctccaatct gggtaaagtc aaacagactg atcgtatcat
     2341 tcatggcatc tatcacagtt cttgcagccg gtttgccgtt gatgtacatg attttttcgt
     2401 tttttgtttc ccaggttcgt gtaatcatta caaaatacca tgtgcccggc ttcagcggtt
     2461 ctgaactgct aacgatacgc catttgcctt gggcactgta tgcgctgtgc tccaggtgac
     2521 catccgaatt cattatcatt aatcgatttt ttttaccctt gtcatagtcg gcataaataa
     2581 attgctgtcg aatggattcg aaataaatcc aaaaaccgat ggaattgaat ccctcgctat
     2641 gctgtggaag ggagcgaaga ttgcctattt gaatggacgg aagtgctccc tgaatacgaa
     2701 agccaagact ttgtttgccg ttgcccggca cagggccggg tacaatagat aagttcggag
     2761 tcgtctgtat ctgaagttca gagtttgata taatatctcg cacggaatca gaattatacg
     2821 caataggtcg tatatatgcc attgcaccta acaatttggc atacatttca aacggggaag
     2881 gaatctcgta tgtaaattta tcgatagagg gcggaataac tgtcagggtt tgcccggaca
     2941 cacgagcggc ctggtttgcc cgcaatcgaa tggattggcc gaatacgcgc gtattagacc
     3001 ctacacgcat ttcaacttcc ccttttctta catgggtctc cgtttgtgcg tattggtcga
     3061 ccagaacccc aagctccgtt ccgtaatcta cgaccagggc agagggggtt cgtaccataa
     3121 agccaatagc ttctttgggc acctgcacag taattttccc ctctgttagc agcagttgat
     3181 cctctccttc aagagtaaac ttgcagggtg attcgattac cacaatactt tgatcattca
     3241 tccggatttt tgcaaaccct ttcttgaggt acaaactctg gtttaggttt aaaatatctc
     3301 cctgccgata atccaatgcg gaatctatcc attcagcatt catagtttca agtacacagg
     3361 cagcaggttc atgttcaatt tgcaggaaag ggacaacccc tatcacaaag atcatcactg
     3421 tggtagtcag cacggctgta aacagggaaa tcttgttgat tttctcagag cgaatccgcg
     3481 gtgctttaac cggagaaata ttttggggat aaggccgtct tggaacttcg actgggcaag
     3541 ctttttgttc tgcttcggcc agagctttcc agaattcggt gtccgcaaaa ttatctgatt
     3601 caaaacattc catcctgtct aaagcatcaa tacgctcttg acgcgagtat aaagaacttt
     3661 gaaaatactt taaatccgcc cagatcaaac taaaatcaac atgaatttgg catgcttctg
     3721 gatcattgct taaccattca tccagttgtt gatactggga atcagtaatt atgccttcgc
     3781 ataatgcatt caaaagtata tataactctt ttttttgttc cggtttcatc ctggcagatt
     3841 ttccttgccg atcgtttttt ctatgcaatg catcaaaagg attcgaatcc tgtatagacg
     3901 gttataaagt gtattaacac tctgccgcaa tcgctcggag acacttttca gggaagcgcc
     3961 tatttcatac cgcaaaatta ataattgccg atcattggtt gaaagttttt cgatgcactt
     4021 tcgcaatgcc caaagacggt catccataga ctccagtctg gctgctaccg tcttctcaat
     4081 ttcctgaagt gtctgagtgc tgaagtgcct ccttgttttt tgttgatttt tgatatgagc
     4141 caaaatcctg ttttttgcta tactaaatgc ccatgctggg aaattggtcc ctatctcaaa
     4201 ttcgttaaaa tgacgccaca tatacgttgc ggtttcctga ataatatctt ctactgaaga
     4261 ttcatcagga acaagcgaaa tgacataggc atacagccga cgctgggcat ctgtgtaaag
     4321 tttcataaac agttccccgc ggtcagcggt taattcatcg tggtttctgt ctttttcaat
     4381 catactgcaa ccgaccggtt ttacgcagaa tttttgcata tgtctatttt taacaatagg
     4441 ttatgtcgtt tcaaaaacac tttaattttt gggcagcatt ccataaaaac cctcattttt
     4501 taatgttttc aagcactttt agatgctgac tcagtcggtt tgagattcat agacatttac
     4561 gttcataagg atagtccgca agaatcggat tttaatgctt aaaaactttc gcatttttgt
     4621 taacatttta ggcggctggg gctaaggcct ccagcaggaa ccggaatttt ttgtcatgta
     4681 cggccgaatt tccgaacatc aattttattc gttcccgcca aagccagcga cgcagacacg
     4741 ccatcgcatc ggcgaagctg cccttggggc cgcaggttt
//
LOCUS       DAOTBO010000163         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_15239_length_4653_cov_5.172901, whole genome shotgun sequence.
ACCESSION   DAOTBO010000163 DAOTBO010000000
VERSION     DAOTBO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15239_length_4653_cov_5.172901"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /locus_tag="PLE88_12345"
     CDS             complement(<1..1932)
                     /locus_tag="PLE88_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOM77296.1"
                     /translation="MHKRTAETVKVSVSIQDKPADLLLSKEWLLTNSRGGFASSTLIG
                     CNTRRYHGLLIGTLTPPASRIAALSNCLEVLTWKDRSISLSSFEFDHVIHPKGLCHML
                     EFRQDTGIHFEYATGPAAVVKSVYLAPDADIAAIVYDFSDVRERFVFTVRPFAALRDF
                     HALQHAQAALLAVGDGNRLCIRCDRPEMGQLVLRCDSMRFEQSPQWWYRFFYRIEQQR
                     GQDCFEDLWSPGQYACSIDEPCRIILWAGLFTDIETSQMLDDIELETVIDALELKEKE
                     LLEQCSTSDPVCQQLCRAADQFIIERHIEDAPAPSILAGYPWFLDWGRDTFIALEGLC
                     LCTGRPKIAWGVLKTFAKAVSQGMIPNRFDDYGGQPHYNSIDASLWFVHAAFRYLRYT
                     KDNQNFSHKLLPAVKWIMDSYRKGTRFGIHADTDMLITGGDVNTQLTWMDAKYDGIAF
                     TPRYGKAVEINALWYSNLCCLADYYRSQNEQEARFYWTLAEQVRHSFQEQFWNSPLGY
                     LNDCILQPGKADTSLRPNQIFAVSLPYSPLTAVHQAAVVAVVEKELLTPFGLRTLNPA
                     DPRYKGRYTGPMSQRDAAYHQGTVWAFLMGPFIEAYLKVHGFDPTAKRRCRGFLSRLL
                     VHINEDACIGSISEIFDGDP"
     gene            complement(1963..3183)
                     /locus_tag="PLE88_12350"
     CDS             complement(1963..3183)
                     /locus_tag="PLE88_12350"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOM77297.1"
                     /translation="MASVCFYFQVHQPFRLRHYTVFDHSPRYFDEFKNASICRKVANK
                     CYLPANRLMLDIIRRLEGRFKIAFSLTGVIIEQFKKYCPEVMSTFDALAETGCVEFLG
                     ETYYHSLSFLYSYDEFIEQINMHTDLMRQLYGQTPRVFRNTELIYSNALAQTIEKMGQ
                     FDAVITEGADHILGGRSSNFVYRPPNCSRIKLLLKNYSLSDDIAFRFGNRNWCEYPLT
                     AEKFASWINRINGNGQCVNLFMDYETFGEHQWEDTGIFSFMSHLPEEVLKHPDNNFMT
                     PSELADTYDAAGVVDVPHIISWADIERDLSAWLGNAMQSNALHEIYKIEKLIKQTHNE
                     ELIADWRRLQTSDHFYYMCTKYFSDGDVHKYFNPYESPYDSYINFMNVLTNLRERCNQ
                     SFLASKKQPLGKIS"
     gene            complement(3234..4586)
                     /locus_tag="PLE88_12355"
     CDS             complement(3234..4586)
                     /locus_tag="PLE88_12355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM77298.1"
                     /translation="MRVFMIGWEFPPYISGGLGTACYGLTKAMNDLGIEVIFVLPKPI
                     DVSPATHVKVLSPLQCSSASAGGKTTIQEWEKEFPNVSFRTIPSPLEPYATPQYYQQK
                     IEGIIRQRTAQAIREKGVEPAVEQWLSKAEEYIQVPYDQYGGDMYSEVHRYAAFAVRL
                     AMQETFDIIHAHDWMTYPAGIAVAAVTGKPLVVHVHSTEFDRSGEHVNQMIYDIERRG
                     MHAASKIIAVSYLTRNIIISRYGILGEKVEVVYNGVEQNGDNRQGFAKVGISSNEKIV
                     LFLGRITMQKGPEYFLMAARKVLEEIENVKFVMAGSGDMMHRIIEMAAQMGIGHKVLF
                     TGFLRGHDVEKVYQMADLYVMPSVSEPFGIAPLEALNHDVPVLISKQSGVSEVLTHVL
                     KVDFWDINEMANKIIAVLKYPPLQLTLREHGNFEVRRLRWSGAAEKCRKIYEGMLQPV
                     "
BASE COUNT         1191 a         1215 c         1111 g         1136 t
ORIGIN      
        1 cggatccccg tcaaaaattt cagatatgct gccgatgcag gcatcctcgt tgatatgcac
       61 cagcaatcgg gagagaaatc cgcggcagcg gcgcttggcg gtgggatcaa aaccgtgtac
      121 cttcaagtaa gcctcgataa acggccccat caaaaaagcc caaacagtcc cctgatgata
      181 ggccgcatcc cgctgactca tcggccccgt atagcgtcct ttgtagcggg ggtcagcagg
      241 attgagcgtg cgaagtccaa aaggcgtcaa caattccttt tctacaaccg caacaactgc
      301 tgcctgatgc acagctgtca gcgggctgta aggcaaagac acggcaaaaa tctgatttgg
      361 ccgcaggctg gtatccgcct tgcccggctg gaggatgcaa tcattaagat accccagcgg
      421 gctgttccag aactgttcct gaaaactgtg ccgaacctgt tccgcaagag tccagtaaaa
      481 acgggcttcc tgctcgtttt gagagcggta ataatctgcc agacagcata aattgctgta
      541 ccacagggcg tttatttcga cggcttttcc atagcggggg gtaaaggcaa tgccgtcata
      601 tttagcgtcc atccaggtca gctgggtgtt gacatcgccg ccggtgatga gcatatccgt
      661 atcagcgtga atcccgaagc gggttccctt gcggtaagaa tccataatcc atttgacagc
      721 tggaaggagc ttatggctga aattctggtt gtcttttgtg taacgaagat accgaaatgc
      781 cgcatgcacg aaccataatg acgcatcaat actgttgtaa tgcggctggc cgccgtaatc
      841 atcaaagcgg ttgggaatca ttccttggct gaccgctttg gcaaaggttt taagcacgcc
      901 ccatgcaatc tttggacggc cggtacagag acacaggcct tccaaagcaa taaatgtatc
      961 gcgaccccag tccaaaaacc aaggatagcc ggccagaatc gaaggtgccg gggcatcctc
     1021 aatgtgccgt tcgataataa actgatctgc cgcccgacaa agctgctgac acaccggatc
     1081 gctggtgctg cactgttcca gaagttcttt ttctttcagt tccaaagcat caataacagt
     1141 ttccagttcg atatcatcca gcatctgcga tgtttcaatg tcggtaaaca aaccggccca
     1201 caaaataata cggcagggtt cgtcaataga acatgcatat tgacccgggg accaaaggtc
     1261 ttcaaagcag tcttgccccc gctgctgttc gatacgatag aagaaacggt accaccactg
     1321 gggactctgc tcaaatcgca tgctgtcgca gcggagcacc agctgcccca tctccggccg
     1381 atcgcagcgt atgcaaagtc ggttgccgtc gcccacagcc agcagagcag cctgtgcatg
     1441 ctgaagcgca tgaaaatccc gcagtgccgc aaaggggcgg actgtaaaga caaatcgttc
     1501 ccgcacatcc gaaaaatcat atacaatcgc agcaatatcc gcatcgggag caaggtaaac
     1561 cgatttgaca acagcagcgg gtccggtcgc atattcaaaa tgtatgccgg tatcctgacg
     1621 gaattccagc atgtggcata agccctttgg gtgtatcaca tggtcgaatt caaaactgct
     1681 cagggaaata ctgcggtctt tccaggtcag cacttccaga cagtttgaca gagcggcaat
     1741 ccggctggca gggggagtca gtgttccaat cagcagtccg tgatagcggc gtgtattgca
     1801 gccgatcagg gtgctggagg caaatccccc ccggctgttg gtcagcagcc attccttgct
     1861 cagcagaaga tctgccggct tgtcctgaat ggacactgaa accttcactg tttctgcagt
     1921 tcgcttatgc atcagcatat ttcccgttcg aaccatcccg gattaagaaa ttttgccgag
     1981 aggctgtttt ttggatgcca aaaaagactg attgcaccgt tcccgaagat tggtcagcac
     2041 attcataaaa ttgatatagg aatcgtaagg tgattcatag ggattgaagt atttgtgtac
     2101 atccccatcg gaaaaatatt tggtgcacat ataatagaaa tggtcggagg tctggagccg
     2161 ccgccagtcg gcaatcagct cctcattgtg cgtctgcttg ataagttttt caattttata
     2221 aatctcgtga agggcattgg actgcatcgc atttccaagc catgcagaca aatcccgctc
     2281 tatatcggcc cagctgataa tgtgcggcac atcaacaacg cctgcggcgt cgtaagtatc
     2341 tgccagctcg ctgggcgtca taaaattgtt gtccgggtgc ttaagaactt cctcgggaag
     2401 atgagacata aacgaaaaaa tgcccgtgtc ttcccattga tgttctccga acgtttcata
     2461 atccataaac aaatttacac attgtccgtt gccattgatg cggttaatcc aagatgcaaa
     2521 tttttcggct gtcagcggat actcgcacca gttgcgattg ccaaagcgaa aggcaatgtc
     2581 atcgctgagc gaataattct tcagcagcag cttgatgcgg ctgcagttgg gggggcggta
     2641 aacaaaattg ctgctgcggc cgccgagaat gtggtcggcg ccttccgtaa taaccgcatc
     2701 aaattgcccc attttttcga tggtttgtgc caaggcattg ctgtaaatca gctcggtatt
     2761 gcgaaacacg cgcggcgtct gtccataaag ctgacgcatc agatcggtgt gcatattgat
     2821 ttgctcgatg aactcatcat aggaatacag aaagcttaaa ctgtggtaat aggtctcccc
     2881 caaaaactcc acacagccgg tttccgccaa agcatcaaac gtgctcatca cctcgggaca
     2941 gtatttttta aactgctcta taatgacccc cgtcaaactg aaggcgattt taaaacggcc
     3001 ttcaagacgg cggataatat ccagcatcaa ccggttggcg ggaaggtagc acttgttggc
     3061 aacctttcgg cagatagaag cgttcttgaa ttcgtcaaaa taacgggggg aatggtcaaa
     3121 aactgtataa tgtctcaagc gaaacggttg atgaacctga aaataaaaac acactgaagc
     3181 catcgcttgt cctcaatccc gtatgcttat gttgccgtat tcttcctgcc ggctcatacc
     3241 ggctgaagca ttccctcata gattttccgg catttttccg ccgctcccga ccaccgcaga
     3301 cgacgcactt caaaattccc atgttcacgc agcgtcagct gaaggggggg gtatttcaga
     3361 acggcgatga ttttattggc catttcgtta atatcccaga aatccacttt cagcacatga
     3421 gtcaggactt ccgacacgcc gctctgtttg ctgatgagca ccggcacatc atggttgagg
     3481 gcttccagag gggcaatgcc gaacggttct gaaaccgacg gcataacata taaatccgcc
     3541 atctggtaga ccttttccac atcatggccg cgcagaaaac cagtaaacag aactttgtgc
     3601 ccgatcccca tctgggcagc catttcgata atccgatgca tcatatcgcc tgaaccggcc
     3661 attacaaact tcacattttc tatttcctca agcaccttgc gggcggccat aagaaaatat
     3721 tccggacctt tctgcattgt gatgcggccc agaaacaaaa cgatcttttc attgctgctg
     3781 atgccgactt tggcaaaacc ctgccggtta tcgccgttct gctcgacccc attgtaaacg
     3841 acttctactt tttctcccag aatgccgtaa cggctgataa taatgttgcg tgtcagatag
     3901 ctgacggcga ttatcttgga ggccgcatgc attccccggc gttcgatatc ataaatcatt
     3961 tgattgacat gttcaccgct tcggtcaaac tcagttgaat ggacgtgaac gaccagcggt
     4021 ttgcccgtta cagcagctac cgcaattcca gccggataag tcatccagtc atgagcatgg
     4081 ataatatcaa atgtttcctg catcgccagc cgaacagcaa aagccgcata ccggtggact
     4141 tccgaataca tatccccccc atactggtcg taagggactt gaatatattc ttcggctttg
     4201 ctgagccact gttcaacagc aggttctaca cctttttccc gaattgcctg ggctgtcctc
     4261 tgtcgaatta taccctcgat tttctgctgg tagtactgcg gtgttgcata aggttccagc
     4321 ggtgagggta tggtacggaa tgaaacatta ggaaactctt tttcccattc ttggatggtt
     4381 gtcttccctc ctgcagaggc ggaactgcac tgcagcggac taagcacctt gacatgggtg
     4441 gcagggctga catcaatggg cttgggcagg acgaaaataa cttctatccc caagtcattc
     4501 attgctttgg tcagcccgta gcaggcggtc cctaaaccac cgcttatata aggggggaat
     4561 tcccaaccga tcataaagac tctcatagtc tcccctcggc tttccaataa actgaacagt
     4621 caaagtccat ttttcctgcg tatttgtacg ccc
//
LOCUS       DAOTBO010000164         4650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_15254_length_4650_cov_6.205876, whole genome shotgun sequence.
ACCESSION   DAOTBO010000164 DAOTBO010000000
VERSION     DAOTBO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15254_length_4650_cov_6.205876"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..865
                     /locus_tag="PLE88_12360"
     CDS             125..865
                     /locus_tag="PLE88_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77299.1"
                     /translation="MNTCRNIILVIFSILFIVLFEGCSAGSGSTAESPKTSEDRRSIE
                     PYFRAAGSGVKSPDADGFIQRWLLLEPIKLPIRSNAGFTESYVRNTICTEYFPDQFTV
                     VPRDGDKVTVSDMELTWHALDSTNFNVKLFRFAYGLNKPTYGILFWAVTVIDCPRQIE
                     NIRLAVGSNSASMWWLNGTEAVGLFGDRRMVMDDCVSKRVTLQKGRNVIRGAVINGPG
                     LSDFCVRFINEQGEPIKDFVVNLETAAK"
     gene            935..2461
                     /locus_tag="PLE88_12365"
     CDS             935..2461
                     /locus_tag="PLE88_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM77300.1"
                     /translation="MKLCYLTALVWGLFLLQPFCPAMTLNGEPYIHDPSTVVQCDGKY
                     YTFGTGGGGLISNDGWTWRSGAVRPGGGAAPDVMKIGDRYLVVYGATGGGLGGGHNGR
                     ILTMWNKTLDPNSPDFKYTEPIEVASSDGIEDCDAIDPSLCLDPKGRLWCTYGTYFGY
                     IRIVQLDPKTGMRVKGNKPVNIAIDCEATALIYRDGWYYLFGTHGTCCSGTHSTYNIR
                     VGRSRKPTGPYLDNMGIDMLRGGGKLVVAANNRLIGPGHFGLIDLGNDVQKFSCHYEA
                     DMDRGGRSVLDIRPLLWKNGWPVAGDMFTGGTFAIESERSGYCLELAVEAVPLSENRR
                     IRQMSSGPRGPGGGAGPGGPGGPSGPGMGRRYSGPVTPIPDQDVAAVSVNWPAGSVGL
                     RLGDYMLQAHQKWTITPVPNVGGYLGSPFFKITIAGTDRALAATEDAELEVVAAFTGE
                     PEQLWRIDQLIDGTYRIIPMAESDSKEPLALTAVSVSTPSLTKFDPRSDKSRWNLRKP
                     "
     gene            complement(2919..>4650)
                     /locus_tag="PLE88_12370"
     CDS             complement(2919..>4650)
                     /locus_tag="PLE88_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOM77301.1"
                     /translation="NVGNPNTRNGPNPGGQSGGDRIYVNAYATGAETGVSWADAYTDL
                     QDALHRASQGCGDEIWVAEGIYRPTTTQNPSISFEMVDGIVLYGGFCADANSLSERDY
                     VKYRTYLSGNVDIEGDSDTYIVVNTEPNSAISSNTLLDGFVITHSTEAGIYCNEADLI
                     IQNCVITDNDANGINVYKASPYLFDSIITDNAAVGVYDCNESHITIEGCIISKNAGNG
                     ISCRKNAALTVKNTWIHNNGGEGIAFSDAASGSVIRNNTIVYNQGKGIWADVTTPPAV
                     TNCIIWGNENGDISGCGATYSCFDLGYQGTGNIDDDPNFIGDAPDVYNYHLRYGSPCR
                     DAGIGYYPEEYDIDQEPREYGPEIDMGADEIYCTDNNLSDDDVYHPLDWNTDGLVNLY
                     EYNYFARAWLSYDPNDPAFDPNDVRYDPNLSEPNSPSFIDPSRFEDWYEWKYICNIDT
                     DGSSAYQIDLADLELFWNNWTWKACYLSFDRWFYTFGEIDGDTDEMMESQTTASTMSF
                     QSGITPIAVEDQLQNAQEVVEFLEKLWLEDPEIQYQIDTQQWTDFMTQIYNWKATLET
                     QYVIEQINFE"
BASE COUNT         1154 a         1112 c         1131 g         1253 t
ORIGIN      
        1 tacttgcagt tcagcggtta attaaattat ttgacagtac ggcatggatt gtatatatca
       61 attttcaaac aagaataggt tttgttttgc ccgcgtaact tcaaagtaat gtgaagggtc
      121 tgttatgaat acatgtcgca atatcatact cgtgatattc agcatccttt ttatagtgtt
      181 atttgaaggc tgttcagccg gatcaggcag taccgccgaa agtcccaaaa ccagtgaaga
      241 tcgccgcagc attgaaccct acttcagggc ggccggatcg ggcgtcaaat cgccggatgc
      301 cgatggtttc atccagcgat ggctgctttt ggaaccaatc aagctgccca tccgcagtaa
      361 tgccgggttt accgaaagtt atgtccgcaa taccatctgt accgagtact ttcccgacca
      421 gtttaccgtc gttccgcgtg acggcgacaa agtgacagta tccgatatgg aactcacatg
      481 gcatgcactg gatagtacca acttcaatgt caagctgttt cgttttgcct acggcttgaa
      541 taagccgaca tacggaattc tgttctgggc tgtgactgtg attgactgcc cgcgtcagat
      601 agagaatatc cgtttggcag tcggttccaa ttccgcgtcc atgtggtggc tgaacggcac
      661 agaggccgtg ggtctgtttg gggatcggcg tatggtgatg gatgactgcg tttccaaacg
      721 cgttacgctc cagaaaggcc gcaatgttat tcgcggtgct gtcattaacg gtccgggatt
      781 aagcgatttc tgtgttcgtt ttatcaatga gcagggtgaa ccgatcaagg atttcgtcgt
      841 gaatcttgaa acggccgcga agtaaattcg tccgggcttt cgttttgtct ggtttaatct
      901 attctaattg cctgttatta tttcaggagg ttccatgaaa ttatgttatt taacagcatt
      961 ggtttggggc ctgtttttac tgcagccgtt ttgtccggcg atgaccctga atggcgaacc
     1021 ctacattcac gatccctcga cagtcgtgca gtgcgacggc aagtattaca ccttcggcac
     1081 cggcgggggc ggactgatat ccaatgacgg ctggacttgg cgaagcggcg ccgtacgtcc
     1141 cggcggcgga gcggcccccg atgtcatgaa aatcggcgac cgttatctgg tggtctatgg
     1201 cgcgaccggc ggcggtctgg ggggtggaca taacggcaga attcttacga tgtggaataa
     1261 gacccttgac ccgaattccc ctgattttaa atacaccgaa ccgattgaag tggcatcgag
     1321 tgacggcatc gaggattgtg acgccattga cccttcgtta tgtcttgacc cgaaaggcag
     1381 actgtggtgc acctacggga cttactttgg ctatatcagg attgtccagc tcgatcccaa
     1441 aacgggcatg cgtgtcaaag gcaacaagcc ggtcaatatc gccatcgact gtgaagcgac
     1501 ggccctgatc tatcgagacg gctggtacta ccttttcggg acccacggca cgtgctgcag
     1561 cggcacccac tctacctaca atatccgcgt cggacgttcc aggaaaccga ccggccctta
     1621 ccttgacaat atgggcattg acatgctccg aggcggcggc aaacttgtcg tggccgccaa
     1681 taatcgcctc atcggtcccg gccacttcgg cctgatcgac cttggcaatg acgttcagaa
     1741 attctcgtgc cactacgaag ccgacatgga tcgcggcggc cgcagcgttc ttgatattcg
     1801 tccgttgctt tggaaaaacg gatggccggt tgccggggac atgtttaccg ggggcacttt
     1861 tgcaatcgag tctgaacgca gcggttattg tctggaactg gctgtggagg ctgtaccgct
     1921 cagtgaaaat cgaagaatcc gtcaaatgtc ttcgggtccc agaggtcccg gcggcggagc
     1981 gggccctggc ggtcccggcg gcccaagcgg cccaggtatg ggcagaagat acagcgggcc
     2041 agtgactccg attccggatc aggatgtcgc agcggtgtct gtaaattggc cggccggcag
     2101 tgttggcctt cgtctgggtg attatatgct ccaggctcat caaaagtgga ctatcacgcc
     2161 cgtccccaat gtcgggggtt atctcggttc cccctttttc aagattacga tagccggtac
     2221 ggatcgcgct ctggcggcta cagaggatgc cgagcttgaa gtggttgcag cctttaccgg
     2281 cgagcctgaa caattgtggc gcatcgacca gttaatcgac ggaacatacc gcattatccc
     2341 gatggccgag tctgattcaa aggaaccgct ggcattgact gcggtgagcg tcagtacgcc
     2401 gtcgctgacc aaattcgacc cccgcagcga caagtcacgc tggaatttaa gaaagccgtg
     2461 atggattagt taattttatt gcattagagc gacaacaaga tcaactatga aaaacgtttt
     2521 atttgtcttt tagtctgctt ctggcaagcc agtaatccca agcaggggtc tatcagcagt
     2581 tggccgtcga ttattgaagg tgataacgta caatattttg cgcacattat tatcgggcat
     2641 attttgcagg tccgttagag tcaaatccaa tagaatttaa atcagatggt gtcgaaatcc
     2701 taaaaggatc ataaatgcct cctgtctgtc cactataggc cgaagacgta caatcagggt
     2761 gttggatgaa tcttactcca aggcgagtaa tgcgaataaa aagaaggcgg cggaggtgct
     2821 ggacaggttg atgggggaaa tataaaaaag gctgtaatcc agtagggggc tacagccggg
     2881 agagggttgt taataggtta atagggagtt ataccttgtt actcaaagtt tatttgttcg
     2941 ataacatatt gtgtttctaa agttgctttc cagttgtaaa tctgggtcat aaaatcggtc
     3001 cactgttgcg tgtcaatttg atactgaatt tccggatctt ccagccataa tttttcaaga
     3061 aattcaacca cttcttgagc attctgaagt tggtcctcca cagcaatagg tgtgattccc
     3121 gattggaaac tcatggtgct tgcagttgtc tgtgattcca tcatttcgtc agtatcccca
     3181 tctatttccc caaaagtata aaaccatctg tcaaaactga gataacatgc tttccaagtc
     3241 cagttattcc agaataactc aaggtctgct aaatcaattt gatacgcact gcttccatct
     3301 gtatcaatgt tgcatatata cttccattca taccaatctt caaaacgact ggggtcgata
     3361 aatgacggag aattaggttc gcttaagttt gggtcatacc tcacatcgtt tgggtcgaat
     3421 gcggggtcat tggggtcata agacagccat gctcttgcga aataattgta ttcatacaga
     3481 ttcacgagtc cgtctgtgtt ccaatccaag gggtgataaa catcatcatc gctcaaatta
     3541 ttatctgtgc agtagatttc atctgccccc atatcaattt cgggaccata ttctcgtggc
     3601 tcctggtcga tatcatactc ctctggataa tatccaattc cggcatcccg gcagggagag
     3661 ccatagcgta gatgataatt gtaaacatct ggggcatcac caataaagtt ggggtcatca
     3721 tcaatatttc cagttccctg atatcccaaa tcaaaacaag agtaagttgc tccacatcct
     3781 gagatgtctc cgttctcatt tccccaaatg atgcaattgg ttacggcagg aggagtcgta
     3841 acatctgccc agattccttt accctgatta tagacaattg tattgttacg aatcacggaa
     3901 ccagaagccg catcggaaaa ggctattcct tccccaccat tgttatgaat ccatgtattc
     3961 ttgaccgtca gtgccgcatt tttcctgcat gagattccat tgcctgcatt ctttgaaata
     4021 atgcaccctt cgatggtgat atgggattca ttgcaatcat aaactcccac agcggcattg
     4081 tcagtaataa tagaatcgaa gagataaggg gaagccttgt aaacattgat cccattggca
     4141 tcattgtctg taatcacgca gttttggata atcaaatctg cctcattaca ataaatcccc
     4201 gcttcggtag aatgcgtgat gacaaaaccg tctaggagag tattactgct tatggctgaa
     4261 ttaggctctg tattgacaac aatatacgta tccgaatccc cctcaatgtc aacatttcca
     4321 ctgagatagg tcctgtattt tacataatct cgttcactca gggaatttgc atcagcacag
     4381 aatcctccat agagcacaat tccatcaacc atctcaaatg aaatacttgg attttgagtc
     4441 gttgtgggac gataaatccc ttcagccacc catatttcat ccccgcaacc ttgtgatgca
     4501 cgatgcaaag catcttgtaa atcagtatag gcatcggccc aggaaacccc tgtttctgct
     4561 ccggtcgcat acgcattcac atagatacgg tctccacctg actgtccccc tgggttaggg
     4621 ccatttcgtg tgttaggatt tccgacattg
//
LOCUS       DAOTBO010000165         4588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_15478_length_4588_cov_6.597176, whole genome shotgun sequence.
ACCESSION   DAOTBO010000165 DAOTBO010000000
VERSION     DAOTBO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15478_length_4588_cov_6.597176"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLE88_12375"
     CDS             <1..501
                     /locus_tag="PLE88_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOM77302.1"
                     /translation="LLRDNPQKVPAGIAAGLKEIRLKDVCNHSYGTICAPIDEATGRH
                     VIRNSIILKKNTPLPCEATQTFYTMSDGQTQLEVTITQGEDEDPDFVNKIATEIFELP
                     PNRPANRPIHVRYSYDVNQRMHCRFLDEESGRTLDVEFCAGGNGQMNRINIERSAEEL
                     SSYKVQ"
     gene            544..1644
                     /locus_tag="PLE88_12380"
     CDS             544..1644
                     /locus_tag="PLE88_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77303.1"
                     /translation="MATDGLARFRRAWPHFSKLFDRYKFACRVDLITDETKPATQDRF
                     FQVQMTGRIPVPQDQYDTDVRIEIRDITDGRNQWSPILSTDDRYRSGENPVFCFQTSN
                     GIVPHRNAVLAGWALVERIPCHILRFAYRGRRKLLFVVSILSAQTGQVLTADQRTMEY
                     VSYCDGYQELRSRRLAALRSSVELAVAVVSEISEEMTALLTAWLGHRASIFMSQEEAA
                     TTVQAIKNQKEQLSVQQSCQPLLAFGDNTDRYGAIELMLRTAGLENRLSRTVFMHLAQ
                     TAELLEIPQNRFYAMAQKILLSSGCIVEDPAQLLGLSEEMEERAFLKRINEEYRKWNA
                     RVTHSDSRIRHQADQMLTLIARLRSQHFQHCS"
     gene            complement(1684..3645)
                     /locus_tag="PLE88_12385"
     CDS             complement(1684..3645)
                     /locus_tag="PLE88_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOM77304.1"
                     /translation="MKSTKVQTALLVFFFIAAIPFLHAEVKLPAFFADRMVLQQNADA
                     TVWGWAEPGEKIVVSADWTKSVKSVRADKNGSWRVKLKTPRAGGPYTLTIAGRNTLVL
                     KDILIGEVWICSGQSNMEFTMAMLRSEAVNADIAKADYPQIRLFTVKKAVAETPQTDV
                     EGSWQICSPQTAAVFSATAYYFGQKLHKELNVPVGLISTSWGGTVAEAWASKEALLRF
                     PAFKQQIEHLGSPDWKASAQRSYEEALKAWEEKIAAMDAGIQEEWFRTDLDDSGWKEM
                     ELPSPWTGTELESVDGIVWFRRATNLPPSWARTSMELHLGPIDDIATVWFNGVNLGTT
                     FGYNKPRVYQVPVAAQRIGKNIIAVRVIDTGYEGGFTGQQEDLRIGPPGADVKTCATV
                     AHTWKYKISSSTAVPAVPADTSVFSQNTPTALYNAMIAPFVGFRIAGAIWYQGESNCY
                     DPILYRTLFPAMIADWRKQWGQGDFPFYYVQIAPYQYNDQTCSQAVREAQMMTLKALP
                     NVGMAVTLDIGQQMDIHPKNKQDVGDRLARWALAQIYGRKDIVYSGPIYKKMKVEDGK
                     IRIFFDYADDGLTARDGDLRDFMIAGPDKNFVPAQAVIDGNTVLVSSEVIEKPAAVRY
                     GWSNWVVGSLFNKAGLPASSFRTDDWPLQ"
     gene            4266..>4588
                     /locus_tag="PLE88_12390"
     rRNA            4266..>4588
                     /locus_tag="PLE88_12390"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1180 a         1169 c         1115 g         1124 t
ORIGIN      
        1 ctgctgcggg acaatcccca gaaggtccca gccgggatcg ctgccggatt aaaggaaatc
       61 cgtcttaaag atgtatgcaa tcacagctac ggcacaattt gcgcccccat tgacgaagca
      121 acaggacggc atgtcattcg caacagcatc atcctcaaaa agaacacccc cctgccctgc
      181 gaagccacgc agacgttcta tacgatgtca gacggtcaaa cccaactgga ggtaaccatc
      241 acgcagggcg aagatgaaga ccctgatttt gtcaataaaa tcgcaacgga aatctttgaa
      301 cttccgccca atcggccggc taacagacct attcacgtgc gctacagcta cgatgtcaac
      361 cagcggatgc actgccggtt tctggacgaa gaatccggtc gcaccctgga tgtcgaattc
      421 tgcgctggag gcaacggtca gatgaaccgc atcaatatag aaaggtccgc agaggaactc
      481 agctcatata aggttcagta acgccgattc actcgaatga gcagcatttt tcaaacaatc
      541 tgtttggcaa cagacggttt agcccgcttc agacgggcgt ggccgcactt cagcaagctg
      601 tttgaccggt acaaatttgc gtgccgggtt gacctgataa ccgatgaaac gaagccggct
      661 actcaagacc gattctttca agtgcaaatg accggccgca tacctgtgcc gcaggatcag
      721 tatgataccg atgtgcgcat cgaaatacgg gatatcaccg acggccggaa tcaatggtcg
      781 ccgatactca gtaccgatga ccgctaccgc agcggggaaa atccggtttt ctgctttcag
      841 acctccaacg gcatcgtccc tcatagaaat gctgtcttgg ccggctgggc cttagtggag
      901 cgcattccct gtcatattct gcggtttgca taccggggtc gccgcaagct gctgtttgtg
      961 gtttcaattc tttccgccca aacaggtcag gtgctgacag ccgatcagag gacaatggaa
     1021 tacgtctctt actgtgacgg gtatcaagaa cttcgaagcc gccgtcttgc agcgctgcgt
     1081 tcgagcgttg aactggccgt tgccgttgtc tctgagattt cggaggaaat gacagccctg
     1141 ctgacggctt ggcttggaca ccgtgcatcc atttttatgt cccaagaaga agcggcaaca
     1201 acagtacaag ccattaaaaa ccagaaggaa cagttgtctg tccagcaatc ctgccagccc
     1261 cttctggcct tcggcgacaa tacagaccgc tacggagcaa tagagcttat gcttcgaacg
     1321 gccggattgg aaaaccgtct gagcaggacc gtctttatgc atctggcgca aacggcagaa
     1381 cttctggaga ttccgcagaa tcgcttttat gcgatggcac aaaaaatcct cttgtcatcc
     1441 ggctgcatag tcgaagatcc cgctcagctg ctcgggctgt cagaagaaat ggaagaaagg
     1501 gcatttctca aacggattaa tgaagaatac cggaaatgga atgcccgcgt tacgcattct
     1561 gacagccgga tacgacatca agcagaccag atgctgacac tgattgcccg acttcgcagt
     1621 cagcattttc agcactgttc ataatcctcc aatcgatagc gattctatcc agcccgccat
     1681 tggctactgc agaggccaat cgtcggtccg aaatgaagag gcaggcagac cggccttgtt
     1741 gaaaagcgaa ccgacaaccc aattgctcca gccgtaccgc acggcagcag gtttctctat
     1801 cacttcactg ctgaccaata ctgtatttcc atcaataacg gcctgtgcgg ggacaaagtt
     1861 cttgtccgga ccggcaatca taaaatccct taaatcaccg tctcgggcgg ttaatccgtc
     1921 atcagcataa tcaaagaaaa tgcggatttt cccgtcttcg accttcattt tcttgtatat
     1981 ggggccggaa tagacaatat cttttctccc ataaatctgt gccaatgccc agcgggccag
     2041 acggtcgccc acatcctgtt tgtttttggg gtggatatcc atctgctggc cgatatcaag
     2101 cgtaaccgcc atccctacat tcggcaaagc tttcagcgtc atcatttgtg cctcgcgaac
     2161 agcttggctg catgtctggt cattgtattg gtagggtgca atctggacat aatagaacgg
     2221 aaagtcccct tgcccccatt gcttccgcca atctgctatc attgccggaa acagcgttcg
     2281 gtagagaatc ggatcgtagc agttcgactc accctgatac cagatagcgc cggcaatgcg
     2341 gaatccaaca aacggtgcta tcattgcatt atacagagcc gtcggtgtat tctggctgaa
     2401 tacagaagtg tctgctggaa cagccggcac agccgttgag gagctgattt tgtatttcca
     2461 cgtatgcgct accgtcgcac aggttttgac atcggcgccc ggcggaccaa ttcgcagatc
     2521 ctcctgctga ccggtaaaac cgccttcata gccggtatca attacacgaa cggcaatgat
     2581 gtttttgcca atccgctggg cggcaacggg gacttgatag acacggggct tgttgtagcc
     2641 gaaggtcgtg ccgagattga ctccattaaa ccagaccgtg gcaatatcat caatagggcc
     2701 taaatgcagt tccatactgg tgcgtgccca cgagggcggc agatttgttg ctcggcgaaa
     2761 ccagacaatc ccgtcgacag attccagctc cgtgccggtc cagggagacg gcaattccat
     2821 ctctttccag ccggaatcgt ccaagtccgt ccgaaaccat tcttcctgta tccctgcatc
     2881 catcgctgca attttttctt cccaagcttt cagggcctct tcataagatc tctgtgcaga
     2941 tgccttccag tcaggactgc cgagatgttc aatctgctgt ttaaatgccg gaaatcgcaa
     3001 cagggcctct ttactggccc aggcctcggc aactgtcccg ccccagctgg tagaaataag
     3061 tccgacaggc acattcaatt ctttatgcag cttctgtcca aagtaataag ccgtcgcaga
     3121 aaaaacagca gctgtttgag gcgagcaaat ctgccagctc ccttcgacgt cggtctgggg
     3181 tgtttcggca accgcttttt ttacagtaaa gaggcggatt tgcggataat ctgctttggc
     3241 aatatctgca tttacggcct ccgaacgcag cattgccatc gtaaattcca tattcgattg
     3301 tcctgaacaa atccacactt ctccaatgag aatatctttg agcacaagcg tatttcggcc
     3361 ggcaatagtc agggtgtatg gaccgccagc acggggggtt ttcaatttca cacgccagct
     3421 gccattttta tctgcacgta ctgatttaac cgacttggtc caatctgcac tgacgacaat
     3481 tttttcaccc ggttctgccc agccccaaac agtcgcatcc gcattctgct gcagaaccat
     3541 cctgtcggca aagaatgccg gcagttttac ctctgcatgc agaaatggaa ttgccgcaat
     3601 gaaaaaaaat acaagcaatg cagtttgaac ttttgtgctt ttcatcggct gcttcctttc
     3661 ctaaaaaagt gactcgctta ttatatccac aactactgct gcagaaataa tttatacact
     3721 atttataaaa tgtcaaccaa cgggtggatt ttctggaagt ttcgtatggt tgggaagttt
     3781 agaatttttt agtttttttc ttgtattctc catatggatt aggtagtatt tcggccctcg
     3841 acgctttcgg cggtcctaat aagagaaaaa tttttgaaaa aatcaaaaat ttttagttga
     3901 cgaataaaaa ataacacgtt aaattacttt tttcgggccg atgaaagcgg acaagtgaag
     3961 acgaaaagtt ggggcttggc cgttttttgt ttccctgaat gggatagcgg ttaatgaagg
     4021 cccaatatat cggaaagctc tttgaaaagt taacagttgg tgccataagc ttaaaagcag
     4081 cgaggcgtag gaaccaagtc tttcacgaca tttgagactt ggatttcctt aaaaccaaat
     4141 tggtgtgata aaccatccgg tttataccgg agcggaatca cttggtttgt cttgctgcgt
     4201 gagcgagatt ttaagttgat tccagagatg caggataccg ttgcttgcaa cggattctac
     4261 aatacaaatt gaagagtttg atcctggctc agaatgaacg ctggcggcat ggctaagaca
     4321 tgcaagtcga acggacatcg taattgggta accgaaagcg gtgttagtgg cgaaagggtg
     4381 aggaatagac aggtaacgta ccccgggctt cggaatagct catcgaaagg tgaggtaata
     4441 ccggataatg tcatttgtat aatgcagatg accaaagatt tatcggcaag ggagcggcct
     4501 gtttcctatc agctagttgg tgaggtaacg gcccaccaag gcgacgacgg gtagcgggac
     4561 tgagaggttg gcccgccaca tcgggact
//
LOCUS       DAOTBO010000166         4580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_15515_length_4580_cov_6.941215, whole genome shotgun sequence.
ACCESSION   DAOTBO010000166 DAOTBO010000000
VERSION     DAOTBO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15515_length_4580_cov_6.941215"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..763
                     /locus_tag="PLE88_12395"
     CDS             293..763
                     /locus_tag="PLE88_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77305.1"
                     /translation="MLFVIDKVRFQKFIAIVRRDRTPITQGKDAPYLRIRAEKDEVEV
                     TSTDASALIQTTVYEPGVLFIRTTLFRQVLKATPTKEPFMTFQVDKAALVFADVRMPF
                     ETNDMVLYTNPAEAPMTFPPPLPEYEKIEEILFQRSLFDMENQINVKEKKDDRK"
     gene            750..1331
                     /locus_tag="PLE88_12400"
     CDS             750..1331
                     /locus_tag="PLE88_12400"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOM77306.1"
                     /translation="MIENNPTNVIAAFEMLLEEIETEIDFINSVGSKAFEKRDYDRAK
                     EALEQAGKLTAFRDKMATLKKEWDSFTQIEMDEEDVEAAQIQRQNLGRLQRGMRTSEE
                     AYYVPILQVLAEMGGSGKVAHVLDKVGVKMQSILKKWDYEPLSSSPDNPRWRNAAQWA
                     RNSMVNEGLLKNDSPRGVWEISEKGKTLLSSRK"
     gene            complement(1788..1994)
                     /locus_tag="PLE88_12405"
                     /pseudo
     CDS             complement(1788..1994)
                     /locus_tag="PLE88_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611912.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2145..2300
                     /locus_tag="PLE88_12410"
     CDS             2145..2300
                     /locus_tag="PLE88_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77307.1"
                     /translation="MNGVIKIDAAQIHSHLDSMVRSTVEETLNKLLDEEADRSGRCTM
                     VPIRARS"
     gene            2916..>4580
                     /locus_tag="PLE88_12415"
     CDS             2916..>4580
                     /locus_tag="PLE88_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77308.1"
                     /translation="MVGKKTCIAAFFMLFVSFIFIGSAAYSESLYVADHGNNVFSFDI
                     VNGAIEFRHSLSVPNSGGGIDLEIDDDHDILFRTVEFASTIDIIEAQSLTYIKTITLD
                     VQPDAGIVYDAANSRLLCTKRNSNRLYIFSWDAALQTLTYQPPYVTLGQIQNACDLAI
                     NGDVLYVSEYIYSGTPSYSELYAYDISDNFSFIGKIDMNHATVSIDYNAGDDSVYGGA
                     WTGHQNLIKRSFDPNTLNYGNIGTSATGVATNGQVAGRVFITSYRDGGSIEWWDLSDA
                     DPNDWELVDDYTNSNSDGVTLSGLAGLAVGRDYIEPTIILEKTDDSSGCVWPEDELTY
                     TLCITNYSETETASGVVLIDYLPDAVDYAGSEYAIDPNFNIIPPDPGYHPDEHAYVWH
                     IGNIAPQDSNTVTLTVEVNYRAEPGMPIENVAVATSDNLGSTVDKLETDICCWDSGTI
                     IYVDKTATGKNNGTSWTDAYTELYKATRRAKDSTCTQDFDIYVAQGTYAPDDTENGFV
                     VPAGVSVYGGYKGGLHDPNNRNPKRYETILTGLIDADAFPDADVIVS"
BASE COUNT         1297 a         1003 c         1135 g         1145 t
ORIGIN      
        1 ccggcagaga aaatgaattg aaatgatgcg gcaaatacag tacaatcaga gcaatacatg
       61 acgtattcag acgctctgaa cagatgtaaa aatgactgcg gacatcgaaa atcctttcac
      121 ctaagctggt acaaagaaaa agggcaggtc agatgtattt gctgctcatg cagcaccgga
      181 cagtaaaaca ctggcgttgt gcaaacaaat gatcgcaaaa acaaacggct atacacaatt
      241 ccgtcatcct ataatgaaac cctcatagag ataggcgcag aaggagtcgc agatgttatt
      301 cgtcattgac aaggttcgtt ttcagaaatt cattgcgatt gtccgtcggg accgaacccc
      361 tataactcag ggaaaagacg ccccctattt acgaattcgg gcggaaaaag acgaagtcga
      421 ggtcaccagc accgacgcct cagccctgat tcaaacaacc gtctatgaac cgggcgtcct
      481 gttcattcgc acaaccttgt ttcgccaggt tctgaaagcc acaccgacta aagagccatt
      541 tatgacattt caggtggata aggctgcttt ggtatttgcg gatgttcgaa tgccgtttga
      601 gactaacgac atggtcctgt acaccaaccc cgccgaagcc cccatgacct tcccgccgcc
      661 attacccgag tatgagaaaa ttgaagagat acttttccag cggagcttat ttgacatgga
      721 aaatcaaata aatgttaagg agaaaaaaga tgatagaaaa taatcctact aatgtcattg
      781 cagcatttga gatgctattg gaagaaattg agacagaaat tgatttcatt aactctgtcg
      841 ggtcgaaagc ttttgaaaaa cgagattatg atcgggccaa agaagccttg gagcaagcgg
      901 gtaaactaac cgcattccgt gataagatgg ccacgttgaa aaaagaatgg gattctttca
      961 cgcagattga aatggatgaa gaagatgttg aagcggcaca aatccagcgt caaaaccttg
     1021 gtcgccttca gcgtggcatg cgcacgtcag aggaagctta ttatgtgccc attttgcagg
     1081 ttctggccga aatgggtggt tccggcaagg ttgctcatgt tctggacaaa gtaggcgtca
     1141 aaatgcaaag catcttgaaa aaatgggatt atgaaccatt gtcgtccagc ccagataatc
     1201 cccgctggcg caacgccgcc cagtgggcca gaaacagcat ggtcaacgaa ggtctattga
     1261 aaaacgacag tccccgtggt gtttgggaga tcagtgaaaa gggcaaaaca ttattgtctt
     1321 cgagaaaata gtgaataata cgatattcct gtaccgtcac aaacaaagcg gcgttaatta
     1381 tggagaatta atagtgtcac agaggaggca aaatgtgttc aaatctgata gggagttata
     1441 atgagcttaa ttaactatga tgctaaagaa ttaggtaatt ataaacgatt ttgcactaat
     1501 ttagtgacat tttggaccaa taaatcgaat agatcagggt ctgttcattt aactgtgtaa
     1561 atggccttaa cggtttcact tcacaattga cattataacc ccttcgcatt aatttgtcca
     1621 tatcgttctg aaggataggg ctctgctgga gagcaacctg agcgtcagga cgatgccgag
     1681 acaggaagct ggtagtgtca agaaagtttc gcacattttc tgagtggctc tcgatctggt
     1741 catggtttcc ctgccttttc cccttaaggg gaaaaatggt tatcggacta tcccgctatc
     1801 atttccacct cttcggattt cttttgttcc cgcaagcgac ccatattcaa ataacacgcc
     1861 gtactccact tggtgccggc gatatgccgc aggcgagccg acaccagcat cagggcactg
     1921 ttgccgtccg ggaagctgcc caccacccgc gtgcgacggc ggatctcttt catgatccgc
     1981 tgcagcatat tagataacgt acactatctt tcgcacatta ttatcgggcc aaaaagaaaa
     2041 gccctcgctc tggtcgatgg aagagtttag aggaacaaaa ccatttggca gagaaagggc
     2101 ctgttatgag cgacaaagaa gcatcggtta acgtgggaaa aaacttgaat ggcgtgatca
     2161 aaattgacgc ggcccagatt cattcgcacc tggacagcat ggttcgcagc acggttgagg
     2221 agacgctcaa caaactgctg gacgaggagg ccgaccgctc aggtcgatgc acaatggtcc
     2281 ccatccgggc acgttcatga tgccgcctgt gtaagcaagg atttccagcc gcggcagctt
     2341 gcgggatgcc tggacagtgt cgattggcag gttgataaat gcaggtggtc cggtgttgtg
     2401 catgaaatct cctgaaaaaa ggtgaatggt tgctctccct gaataatacg tcgaatcagg
     2461 gggcgttgcg cagttacgaa gaaccaatct tcattattcg ctccgacctc tatatctgca
     2521 gccggggcga ttttgtcgca tggaaaattg ttttgaggcg gccattaagt gtaagtatca
     2581 ttaacgtaat gagttaagcg gcaagtgaac tggcggcgat aatttgccgc ctttttttcg
     2641 attgaccgat aataagtggt tttatttcat tattgcgtaa aactgaatac ttgaaagtcc
     2701 actggacttt cgtgtctccg tctaaaccgg caaagttttg tgcaaagaga tgcgaaggtc
     2761 agtgccccac gccggggcac tgccgaagca tgttctttgc tggccttgcc gggccaagac
     2821 ggagcatgaa gtaggtagtg ctcctttttt acggggctgg cctgtaagag gggttaagtt
     2881 ggattttggg gggattaacg aaaggagaac tggtcatggt cgggaagaag acttgcatcg
     2941 cggcgttttt catgttgttt gtatccttta tttttatagg ctccgcagca tattcagagt
     3001 cgctgtatgt ggcggaccac ggcaataatg tcttttcctt cgatattgtt aatggtgcaa
     3061 ttgaattcag acattcgctc tcagtgccaa attcgggcgg aggaattgat ctcgaaatcg
     3121 acgacgacca tgacatcttg tttaggaccg tcgagtttgc cagtacaatt gacattatag
     3181 aggcacagtc cctgacctac atcaagacaa ttacgctgga cgtgcagcct gacgccggga
     3241 tcgtgtatga cgctgctaac tccagattgc tgtgcacaaa aagaaactcc aatcggcttt
     3301 acatattctc atgggatgcc gcactccaga cattgaccta tcagccacca tatgttacgc
     3361 ttggacaaat tcaaaacgcc tgtgatttgg caatcaacgg agatgttttg tatgtctccg
     3421 aatacatcta ttccggcact ccctcatatt ctgaacttta cgcttacgat atttcagaca
     3481 atttctcttt tattgggaaa attgacatga accatgcaac cgtcagcata gattataatg
     3541 ctggcgatga tagcgtttat ggtggagcat ggacgggaca ccaaaaccta attaaaagaa
     3601 gttttgaccc taacacgtta aactacggca atattggaac atccgctacc ggagtagcaa
     3661 caaacggtca agttgcagga agagtgttta taacgagcta tcgggatggc ggctcaatag
     3721 aatggtggga tctgagcgat gccgacccga atgattggga actcgtggat gattatacaa
     3781 acagcaattc cgatggggtt acgctttcgg gacttgcggg attggctgtg ggcagagact
     3841 atatagaacc cacaattatt ctggagaaaa cggatgattc gagcggctgt gtgtggcccg
     3901 aagatgaatt aacctataca ctgtgcatca caaactattc tgaaacggaa acagcatcag
     3961 gggtcgtatt gattgattat ttaccggatg cggtggatta cgcaggttca gagtatgcca
     4021 tcgatcccaa tttcaacatt attccgcctg accccggata ccatcctgat gaacatgcgt
     4081 atgtctggca catcggcaat atcgcaccgc aggattcgaa tacggtgacg ttaacggtcg
     4141 aggtgaatta cagggcagag ccgggaatgc ccattgaaaa cgtagcggtt gccacttccg
     4201 acaatctcgg ttcgactgta gataagctcg aaaccgatat ttgctgctgg gattccggaa
     4261 cgattatcta tgtcgacaag acagcgaccg gcaaaaacaa tggtacaagc tggacggatg
     4321 cttataccga attatacaaa gcgaccagaa gagccaaaga ttcaacttgt acgcaagatt
     4381 ttgatattta cgtcgcacaa ggaacgtatg cgccggatga cactgaaaat ggatttgttg
     4441 tgcccgctgg tgtctctgtt tatggcggtt acaaaggtgg gctccatgat ccaaataacc
     4501 gcaatccgaa gagatatgag actatcctga cgggcctgat tgatgcagat gcgtttccgg
     4561 acgcagacgt aattgtctcg
//
LOCUS       DAOTBO010000167         4332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_16547_length_4332_cov_4.018237, whole genome shotgun sequence.
ACCESSION   DAOTBO010000167 DAOTBO010000000
VERSION     DAOTBO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16547_length_4332_cov_4.018237"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..2076)
                     /locus_tag="PLE88_12420"
     CDS             complement(49..2076)
                     /locus_tag="PLE88_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77309.1"
                     /translation="MKTAQYKMGFIGVVLVCGTCFAARTYVDARGGAGGNTVNAVTGS
                     PTDWWVSSPSQDNLWGRRSGFANNPNFTLGGDSDVFESSGTGSGRENSPMVKTVLSGL
                     QAGMYYNLWVVYWSANGQNWCIRAGLTPANLPLYDFTGATGATAGTATGKIEGDRNEL
                     TASLGYAAADSSGTLSVYVDDKPSDASQGGWYDRTWYDGILYEVYNIPFAPSPADGAV
                     NVALSASVLSFMPMRDPNNLSRENPKILLHKVYFDDDPNLADAEDLLASLPAGLSQTA
                     MPALQNNKTYYWRVDELLKDNTVIAGMVWSFQTVKTVPDFNPPIGYQPVSTAIFPGET
                     AVLSAAAGAGDFNYQWYKGTSGDTSQPLSDEAEHIWGASSSQLSIRALPEDEGFYWCR
                     AGNELGTADSQAAGITVKRLTAHWTLNLSNYHDGRYEDITGEGHPVIVQGSPAFFEGV
                     DGQAAGAVQIDSANGFGNSGTWNPSAISGRLTISLWAWWAGPTDPVSWQGLMAKRNAF
                     TEEGMMWQLEVDQSNNNVVYKNGSNQLVSSDPLPIGQWAHVAVTFDGTTVSLYRNGEL
                     VSSGVVPFSGGTAANLVLGATELSDADVFSWPFHGGLDDVRIYNYALDAAAVAQLYVD
                     FVPDASICLGNPEMDFSGPLGEPDCRVDLYDLAALARQWLVCSLIPSSACY"
     gene            complement(2139..4124)
                     /locus_tag="PLE88_12425"
     CDS             complement(2139..4124)
                     /locus_tag="PLE88_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77310.1"
                     /translation="MKSIPFRIFLVLFWLVCAAGVWGWTGSVYQGVYADASESNTAAV
                     SGQIPWNSGTGDAAGLWKLRTVCGNEMTLWEANGSTSYLGDCPMLVTTISGLLPGFRY
                     AVSVVYWNPSGQNIRLMAGLQPSSLAEFNTNNESSRQTGAVDADRYEYEGLIGIAQAD
                     GTGRISVYIDDIPSTYRSWYDGLTYHALGAFAPIPAEEAVDVPGGQNLTLYWSMPADP
                     ANPTSPSPYVTGHYLYIRKNDPNLLGAVPLAVPVTPDPTAQSWTVDVENNAIYYWRVD
                     ESIRSSGPKDPNTIQGEVWSFRTTQTIPSFEPPYGTQPTGIAVFENQLAVLTAQAGVS
                     PGQDDAFLYQWYKGRSGITSSPVSDEPGHIEGSAGSQLRIYVRPEDAGYYWCRAVNSS
                     GSADSQAAKIFLKKLIEHYAFDGNLNDSAGTNHGTMSGAVYIAGINGQALVFDGTRYM
                     SIGTAGYPNSSEEGGLAEGTVSFWINSSVTGAHSFIGNANTSDGTMLNIHYSNSNALQ
                     LYVQNAASSQMLLQFSSPINLRNGQWHLLTFAWDCAAGQGRIYLDGRLAYSGTATAVS
                     GFTGWQYPMYIGAQNNGGTAASFYNGALDDLRVYNYPLTAAEAAQLYVDFVPGTEICF
                     ENPIYDFNGDCRTDIADLIEFAAGWMECNLIGANACR"
BASE COUNT         1014 a         1285 c         1066 g          967 t
ORIGIN      
        1 tggggtctgt accgacgggc acagaccccg gtttcaatac cttttatttc aataacacgc
       61 agaagaagga atcaaactgc acaccagcca ctgacgggct aaagccgcca aatcatacaa
      121 atctactctg caatccggct ctcccagcgg accgctgaaa tccatctcgg gattgccaag
      181 gcagatggac gcatccggca caaaatccac atacagttgt gcaacggctg ccgcatccaa
      241 agcataatta taaatccgca catcatccag accgccgtgg aacggccagc tgaagacatc
      301 cgcatcgctc agctccgtag ctcccagaac cagattagcg gcggtaccgc cgctgaaggg
      361 gacgacgccg gaggagacca actctccgtt gcgatagaga gaaaccgtcg tgccgtcaaa
      421 agtaaccgcc acatgggccc attggccgat tggaagcggg tcggaggaaa ccaactgatt
      481 gctgccgttc ttgtaaacca cattgttgtt tgactggtcc acttccagct gccacatcat
      541 cccttcttcg gtaaaggcgt tgcgttttgc catcaagccc tgccaggata caggatccgt
      601 ggggcctgcc caccacgccc acagcgaaat tgtcagccgg ccgctgatag cggaaggatt
      661 ccaggtgccc gaattgccga aaccgtttgc actgtcaatc tgaacggcgc cggcggcttg
      721 tccgtccaca ccctcaaaaa aggccggact tccctggaca attacaggat ggccttcgcc
      781 ggtgatgtct tcatatcggc cgtcgtgata attggaaagg ttcagcgtcc aatgagcggt
      841 caatcgtttg accgttatgc cggctgcctg ggaatctgct gtaccgagtt cattgccggc
      901 acggcaccag taaaaccctt catcttccgg caaggcccta atcgacaatt gagagctgga
      961 agccccccaa atatgctctg cttcatcgga caagggctga ctggtatcgc cggaggtccc
     1021 cttataccac tgatagttaa aatcgccggc tcccgccgcg gccgagagta ccgccgtttc
     1081 cccagggaag atcgctgtgc tgaccggctg gtatccaatc ggcggattaa aatcaggaac
     1141 ggtcttcact gtctggaatg accataccat acccgcaatc accgtattgt ctttcagcag
     1201 ttcatccacc cgccagtaat aggttttatt gttctggaga gccggcatcg ccgtctggct
     1261 gagtcccgcc ggcagagacg ccagcaaatc ttctgcatcc gccaggttgg gatcatcatc
     1321 aaaataaacc ttgtggagaa gaatcttcgg attttcccga ctcagattgt tcgggtcccg
     1381 catgggcatg aaggatagaa ccgatgcaga aagagccaca tttacggctc catctgccgg
     1441 cgagggtgca aagggtatat tataaacttc ataaagaatg ccgtcatacc aggtgcgatc
     1501 ataccagccg ccctgggatg catccgaggg cttgtcatct acatagaccg acagcgttcc
     1561 gctgctgtcg gcggcggcat accccagcga tgccgtcagt tcattgcggt cgccttcgat
     1621 ttttccggtc gccgttcccg ctgttgcgcc ggtcgcccct gtaaaatcat aaagcggcag
     1681 attggccggc gtcaggcctg cccgtatgca ccagttctgc ccattggctg accagtacac
     1741 cacccacaga ttataataca tgcctgcctg cagccctgaa agcactgtct tgaccatagg
     1801 gctgttttca cgtccgctgc ctgttccgct ggattcaaaa acatcgctgt ctccgcccag
     1861 tgtaaaattg ggattgttgg caaaaccgct tcgccgaccc cagaggttgt cctgcgacgg
     1921 tgatgaaacc caccagtccg ttgggctgcc ggttaccgca ttcactgtat tgccgccagc
     1981 tccgccgcgg gcatccacat aagtgcgggc agcaaaacac gtcccgcaga ccaggacgac
     2041 tccgataaaa cccattttat attgtgccgt tttcatggtt tactctccta tcctttccta
     2101 tcatcaaacc ccttgccatg ctatatcgcc ttcgcctttt accgacatgc gtttgccccg
     2161 atcagattgc actccatcca tccggcagca aattcaatca ggtctgcgat atccgttcga
     2221 cagtccccat tgaaatcata aatcggattt tcaaagcaga tttccgttcc gggcacaaag
     2281 tccacataga gttgggcggc ttcggcggct gtcagcggat agttatagac gcgcaggtca
     2341 tcgagcgctc cattatagaa ggaggcagct gtgccgccgt tgttctgagc gccgatgtac
     2401 attggatact gccagccggt aaatccactg acggccgttg ctgtgccgga ataggccaat
     2461 ctgccgtcca gataaatccg cccctgcccg gccgcacaat cccaggcgaa cgtcagcaga
     2521 tgccactggc cattgcgaag gttaatcgga gaagaaaact gcaagagcat ctgagaggaa
     2581 gccgcattct gaacatacaa ctgcagcgcg ttgctgttgg aataatggat attcagcatg
     2641 gttccgtcag aggtattggc gttgccgatg aaactgtggg ctcccgtaac ggaggagttt
     2701 atccagaacg aaacggttcc ctccgccaaa ccgccttctt cgctgctgtt gggatatccg
     2761 gcggtgccga tgctcatata gcgggtaccg tcaaagacga gggcctgtcc gttaatgccg
     2821 gcgatataaa ctgctccgct catcgtgccg tgatttgtcc ccgccgagtc attcagattt
     2881 ccgtcgaatg cataatgttc gataagtttt ttgaggaaga ttttggctgc ctgagaatcc
     2941 gcactgccgc tgctgttgac ggcccgacac caataatacc ctgcatcttc gggccgaaca
     3001 taaatccgaa gctgtgaacc ggctgacccc tcaatatgtc cgggctcgtc cgaaaccgga
     3061 gagcttgtta tgccggaccg gcccttatac cattgataaa ggaaggcatc atcctggccg
     3121 ggtgaaacac ccgcttgagc cgtcagaaca gccagctgat tttcaaaaac cgctatcccc
     3181 gtcggctggg tcccgtaagg cggctcaaaa gacgggattg tctgtgttgt ccgaaaagac
     3241 catacttccc cctgaatcgt attggggtct ttcggccctg aagaacggat actctcatcc
     3301 accctccagt aataaatcgc attattctcc acgtcaacgg tccagctttg cgccgttggg
     3361 tccggcgtga ccggcacagc caacggaacc gcccccagca gattgggatc attcttgcga
     3421 atataaagat agtgtcctgt tacataagga ctcggcgatg tcggattggc cgggtcggcc
     3481 ggcattgacc aatagagggt cagattctgt ccgccgggaa catccactgc ttcttcagcg
     3541 ggaatgggag caaaagcccc caacgcatga taagtcaggc cgtcatacca gcttcgatac
     3601 gtactgggaa tatcatcgat ataaaccgaa atccggccgg tgccgtccgc ctgggcaatg
     3661 ccgataagtc cctcatattc atagcgatct gcatccacgg cgccggtttg acggctgctc
     3721 tcattattcg tgttaaactc ggccaaactg ctcggctgca aaccggccat cagccgaata
     3781 ttctgaccgg atggattcca gtacaccaca gaaacggcat atcggaatcc cggcagcaaa
     3841 ccggaaatcg tcgtaaccag catcggacaa tcgccgagat aggatgtgct gccgttggcc
     3901 tcccaaagcg tcatctcatt gccgcagaca gtgcgaagtt tccaaagacc tgccgcatcg
     3961 cctgttccgc tgttccaggg aatttggccg ctgaccgcgg cggtattgct ttctgaggca
     4021 tctgcataaa ccccctggta aacactcccc gtccagcccc atacgccggc agcgcaaacc
     4081 agccaaaata gaaccagaaa gattctgaat ggaatgcttt tcataagtaa cccctttctg
     4141 aaaaataccc gcctttactc actaacaaca actcctaaaa ccgctcttca tcccaaaaat
     4201 taccctgtca aggcaagcgt ggattttcgc ctcccccgcc ggctgatatc caaccttctt
     4261 tgggttattt ccaccacctg ccttccgaat taattcccct gcggcttgcc tgcttcgtaa
     4321 tagcatcgca gt
//
LOCUS       DAOTBO010000168         4248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_16929_length_4248_cov_6.356308, whole genome shotgun sequence.
ACCESSION   DAOTBO010000168 DAOTBO010000000
VERSION     DAOTBO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16929_length_4248_cov_6.356308"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLE88_12430"
     CDS             complement(<1..728)
                     /locus_tag="PLE88_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOM77311.1"
                     /translation="MAAVVYMDNNATTKVADEVIEEMMPYLRDKYGNPSSMHTFGGQV
                     AKKIAWAREQVAALLGCEPDEIVFTSCGTESDNTAIKGTLEMYPQRRKVVTTRVEHPA
                     VLTTCRELERHGFSVSEIGVDRQGNLDLDELGFRLDDNTAIVSVMYANNETGVIFPVE
                     QVAQLAAARGIVFHTDAVQAVGKIPINLKNSAIHLLSLSGHKIHAPKGIGVLYVRRGT
                     RLGTFMHGGHQESGHSGRTGSAAL"
     gene            complement(754..1605)
                     /gene="nifU"
                     /locus_tag="PLE88_12435"
     CDS             complement(754..1605)
                     /gene="nifU"
                     /locus_tag="PLE88_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOM77312.1"
                     /translation="MWDYTNKVIDHFRNPQNVGRLDDPDGVGEVGSIACGDALRLMFK
                     LDENGRIKDVKFQTFGCASAIASSSVLTEMIKGMTLEEASRVTNKDIVERLGGLPSQK
                     MHCSVMGQEALEAAIQDYYKRKGGKAPEAKKGTIVCTCFGVTDEEIKKVVRQNNLITV
                     EDVTNYCKAGGGCGGCRERIAELIQEVRGERPLPPMSPAKPARLTNIQKIQLIQQTIN
                     EQIRPALRADGGDIDLIDVEGNKVIVAFRGMCAQCRMAEYTMKDVVEARLKEYVSGDL
                     YVEEMKD"
     gene            complement(1629..2918)
                     /locus_tag="PLE88_12440"
     CDS             complement(1629..2918)
                     /locus_tag="PLE88_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOM77313.1"
                     /translation="MDNAKKYQLDTLALHAGQEIEQTTRSRAVPIYQTTSYVFENADH
                     AANLFALKEFGNIYTRLMNPTTDVLEKRIAALEGGIGACAFSSGMAAITAAILNIAPA
                     GSHVVASSSLYGGTFTLFTHTLPQMGIEFTLVDPGSPDNFERAIQHNTKLIYIESIGN
                     PKNDVLDYEAIAAMAHRNGLPLICDNTVTSPMLFRPIEHGIDIVVHSCTKMIGGHGTS
                     IGGIVVDSGKFKWDNGKFAGLTKPDPSYHGLKYYEVFGALAYIVKIRTHILRDTGACL
                     SPFNAFLLLQGIESLHLRVPRHCENALALARFLEKHPAVSWVNYPGLPSHPNYKLAQQ
                     YLPKGQGAILGFGIQGGIESGKKFIENVKLASHLANVLDSKTLVIHPASTTHQQLTES
                     QQRAAGVTPDFIRVSVGTEHIDDIIDDFEQALRASQR"
     gene            complement(2948..3859)
                     /gene="nadA"
                     /locus_tag="PLE88_12445"
     CDS             complement(2948..3859)
                     /gene="nadA"
                     /locus_tag="PLE88_12445"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOM77314.1"
                     /translation="MSPEIIEKIRTLKKQRKAVILAHNYQPGPIQDIADFTGDSLGLS
                     IEAAKTDAEVIVFCGVRFMAETAAVLSPDKTVLLPAQDAGCPMADMIDASQLRQLKAQ
                     YPGALVVCYVNSTAEVKALSDYCCTSSNALQLVRSLPEDRDIIFVPDKNLGGFVAEKT
                     GRPLILWPGYCSTHVCILPEDVIMARREHPAAVVLAHPECTPQVRAAADELLSTGQML
                     KFAKASSAQEFIIATEKGILHTLRKENPDKRFYAVSQKAVCPNMKKITLEKVVWALED
                     MQYQITVPASIRAEAKKALDRMLEVTP"
     gene            complement(3856..>4248)
                     /locus_tag="PLE88_12450"
     CDS             complement(3856..>4248)
                     /locus_tag="PLE88_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOM77315.1"
                     /translation="TGIDIHPGAQIGRYFFIDHGTGVVIGETAVIGDHVKIYQGVTLG
                     ALSFPKDERGQIIRGRKRHPTLEDDVTVYAEATILGDITVGRGAVIGGNVWIRESVPA
                     GTIVTITKPEALYRKKGPGFDDRLEYYL"
BASE COUNT          986 a         1170 c         1002 g         1090 t
ORIGIN      
        1 tcgagtgccg ccgatcctgt gcgcccgctg tggccggact cctgatggcc gccatgcata
       61 aatgtaccca gccgcgtgcc ccgccggaca tagagaactc cgatgccttt gggggcgtga
      121 atcttgtgac cggacaggct caacagatga atagcgctgt ttttcagatt gatggggatc
      181 ttgccgaccg cttgaaccgc atcggtatga aaaacaatgc cccgcgccgc cgccagctgg
      241 gcaacttgct cgaccgggaa aattacaccg gtttcattgt tggcatacat cacagacaca
      301 atagcagtgt tgtcgtcaag ccgaaatccc agctcgtcaa gatccaaatt gccttgtcgg
      361 tcaacaccga tttcgctgac gctgaatccg tgacgctcca gttcccggca ggtagtcagc
      421 acggccggat gctccacacg agtggtcaca acctttctcc gctggggata catttcaagt
      481 gtgcctttga tagcggtgtt gtcgctttcg gtgccgcagc ttgtaaagac aatttcatcc
      541 ggctcacatc caagcagcgc ggcgacctgt tctctggccc atgcaatttt tttggctacc
      601 tgccccccga atgtatgcat gctggacgga ttaccatact tgtcccgcag gtagggcatc
      661 atttcttcga tgacttcgtc ggccactttg gtggtcgcat tgttatccat atatacaaca
      721 gcagccataa gcagtgctcc cggaaatttg cttttagtct ttcatttctt caacatacaa
      781 atcgccgctg acatattctt taagccgcgc ctcgaccaca tctttcatcg tatattcggc
      841 cattctgcac tgggcacaca tcccgcggaa ggccacaatg accttattgc cttccacatc
      901 aatcaggtca atgtcgcccc cgtcagctct cagagcagga cggatctgct cattgatagt
      961 ctgctgtatg agctggattt tctgaatatt ggttaatcgg gccggcttgg caggagacat
     1021 cggcggcaag ggacgttctc cgcggacttc ctgaatcagt tcggctatcc gttctcggca
     1081 tcctccgcag cccccgccgg ccttgcagta gttggttaca tcctcgacgg ttatcagatt
     1141 gttctgccgt accacttttt tgatttcttc atcggttact ccgaagcagg tacagacgat
     1201 ggtccctttc ttcgcctccg gtgcttttcc gcctttgcgc ttgtaataat cctgaatcgc
     1261 cgcttcaagg gcttcctgcc ccataaccga acaatgcatt ttttgactgg gcagcccgcc
     1321 gagacgttct acaatatcct tattggtcac ccgtgaggcc tcctcaaggg tcattccctt
     1381 aatcatttcg gtcagcaccg aagaagaagc aatcgcgctg gcacagccga aggtttgaaa
     1441 cttgacatct ttaatccgac cgttttcgtc cagcttaaac atcagacgaa gtgcatcgcc
     1501 gcaggcaatt gaaccaacct cgcccactcc gtcagggtca tccagcctgc cgacattctg
     1561 cggatttcgg aaatgatcga tcaccttgtt tgtataatcc cacataaccg tattcctata
     1621 aggatatgtt atcgctgact ggcccttagg gcctgttcaa aatcgtcaat aatatcatca
     1681 atatgctcag tcccaacgga cacacgaatg aaatcgggcg taacgccggc agcacgctgc
     1741 tgactttcgg tcagctgctg gtgtgtcgtg ctggccgggt gaatgaccag cgtcttgctg
     1801 tccagcacat tggccagatg gctggccagt ttgacattct cgataaattt ctttccactt
     1861 tcgatgccgc cttggatgcc gaaaccgaga attgccccct gccctttcgg cagatattgc
     1921 tgagccagtt tataattggg atgcgacggc aggcccggat aatttaccca gctgacagcc
     1981 ggatgctttt ccagaaaccg tgccaacgcc aaggcatttt cgcaatgccg gggcactcgt
     2041 aggtgcaatg actcgattcc ctgaagcagc agaaacgcat taaagggcga taagcatgcc
     2101 ccggtatccc gcaaaatatg ggtacgaatt ttgacaatat aagccagcgc accaaagacc
     2161 tcataatatt tcaaaccgtg atagctgggg tccggcttgg tcagaccggc aaacttgccg
     2221 ttatcccatt tgaatttccc ggaatcaact acaatcccgc cgatgcttgt accatgtccc
     2281 ccgatcattt tggtacagga atgaaccaca atatcgatgc cgtgctcaat agggcgaaac
     2341 agcatcggag atgtgactgt attgtcgcaa atcagcggca gaccattccg atgagccatt
     2401 gccgcaatgg cttcataatc cagcacatca ttcttaggat ttccgatgct ctcaatgtaa
     2461 ataagcttgg tattatgctg aatcgcccgc tcaaaattat ccgggcttcc ggggtcaaca
     2521 agggtaaact cgatgcccat ttgaggcagg gtatgtgtaa acagcgtaaa ggttccgccg
     2581 taaagcgaac tggaagcaac cacatgcgac ccagccgggg caatattgag gatggcagca
     2641 gtaattgccg ccataccgga gctgaaggcg caggccccga tgcccccttc caacgcggca
     2701 atccgctttt ccagaacatc ggtcgtagga ttcatcagcc gggtataaat gttgccgaat
     2761 tctttcagcg caaacagatt ggccgcatgg tcagcatttt caaatacgta gctggtggtt
     2821 tggtatatcg gcaccgcccg acttcgtgtt gtttgttcga tttcctggcc ggcatgcaga
     2881 gccaatgtat ccaattgata tttttttgca ttgtccattt ggcagctttc tttttacaac
     2941 gtcgttatta tggagttact tcgagcatcc gatccaaagc ttttttagct tctgcccgga
     3001 tagatgccgg cacggttatc tgatattgca tatcctccaa agcccagacc actttttcga
     3061 gagtaatctt tttcatattg ggacaaactg ctttctgcga tacagcataa aaccgcttat
     3121 ctggattttc cttgcgcaac gtatgcagaa tccctttttc cgtggcgatg ataaattcct
     3181 gtgcggaact ggcctttgca aatttaagca tctgaccggt actgagaagc tcatctgcag
     3241 cagcacggac ctgcggggtg cattccggat gagccaaaac taccgccgct ggatgttctc
     3301 ggcgtgccat tatcacatct tcaggcagga tgcatacgtg cgttgagcaa taaccgggcc
     3361 acaaaatcaa agggcgtcct gttttttctg caacgaatcc gccgagattt ttatcaggca
     3421 caaaaataat gtcgcggtct tccggaagag aacgcaccag ctgaagcgca ttgctgctgg
     3481 tacagcagta gtcgctgagg gctttaactt cggccgtcga atttacataa cagacaacca
     3541 aagctccggg atactgggct tttaactggc gaagctgaga ggcatcgatc atatcggcca
     3601 ttggacagcc ggcgtcttgg gccggcagca gcaccgtctt atcaggcgac aacacagccg
     3661 ctgtttcagc cataaaccgt acaccgcaga aaacaataac ctccgcatcc gttttggccg
     3721 cctcgatgct taatcccagc gaatctccgg taaaatcagc gatatcctgt atgggtccgg
     3781 gctgataatt atgggccaag atgaccgctt ttcgctgctt ttttaatgtt cggatttttt
     3841 cgataatttc cgggctcaca aataatactc caaacgatca tcaaatccgg gacctttttt
     3901 tctgtataac gcctccggtt ttgttatcgt cacaattgtg ccggcgggta cagactcccg
     3961 aatccagaca ttgccgccga taacagctcc cctgccgact gtaatatctc ctagaatcgt
     4021 cgcttcggca tagacggtta catcatcttc caatgtcggg tggcgcttcc ggccgcggat
     4081 aatctgcccc cgctcatcct tgggaaaact caaggcgcct aaagtaacac cctgatagat
     4141 tttaacatga tcaccgatca ccgctgtttc gccaattaca acaccggttc cgtgatcaat
     4201 aaagaaatag cggccgattt gggcgccggg gtgaatgtcg atgcctgt
//
LOCUS       DAOTBO010000169         4223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_17067_length_4223_cov_3.984645, whole genome shotgun sequence.
ACCESSION   DAOTBO010000169 DAOTBO010000000
VERSION     DAOTBO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17067_length_4223_cov_3.984645"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..598
                     /locus_tag="PLE88_12455"
     CDS             167..598
                     /locus_tag="PLE88_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77316.1"
                     /translation="MKLALDIADAIVTELAGNNFSEPLVVTRRVLPEYEIAELKALTF
                     TVVPKSVEIINITRQSSSFDVAIDIGIQQKIGKDTDAEATRLSGIVTEVVSFLNRRKL
                     AGFPAAVFVSIANEPVYAPEHLSEKRLFTSILTLTYKVIDG"
     gene            591..902
                     /locus_tag="PLE88_12460"
     CDS             591..902
                     /locus_tag="PLE88_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77317.1"
                     /translation="MANQIEQLTTWMVNRANQQGLSIQQFKDIKFQHVRSNLPAAYRD
                     RLTQAVYIKAKRLALTQLFQEKLDQLKNNTNVRQTILTVFPDATFRVYPNKRQIIIEV
                     Q"
     gene            902..2701
                     /locus_tag="PLE88_12465"
     CDS             902..2701
                     /locus_tag="PLE88_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77318.1"
                     /translation="MTQIYHKKACCWFYKSGSGSNQAGGCTKEFLDNHNGDVSLVMGP
                     AGQPKQNANADAINDFNTETAQLTASGLDTNVLAGLVARINNSFCEVVSVGLDTVGID
                     QWAGKSEYIWTDTNETNSIIYPDDDSIYSVGGYIHIKGTNGQLADGTYEILSMEYGGL
                     EIDDGMAVFDNDWGYLYCAPESMSGVNIVIGGAYNDLQDVLNIPDAVYHDQWIFFNEE
                     IAPSVLVNGSAYYIYSSHDGEAANNTKLYIVGYNTYAYDCLPDGYGYFPEGTQNVSTH
                     YKTALERAKNPSDTSIKADLPTATTKNITDNWGRIFRVYTSAENVQFMGIYFEVAVYH
                     WPIMADNETTGSVFVKHCAGGHISYDAAPAFYGGQIVRVNDAATGGGIFDSFFKGVEM
                     FNGTTPTVDSSGDWEFAYNVGIYTSNINPDYSGPVVHHNIFVKSNWGVSAVARSFQNV
                     FNNIFYLCRQAGLHLNGTEARMRAWNNIIVMDETSGIFDGLFRISAGGTVDYLDYNCY
                     CNQNGQPVSIFAFDTEANPSFNFDNLKKGPHDIEADPMFMDPDNWDFRLRENSPCIGM
                     GRPDLQGNPATIGPYLEKQTVGLYGKTKSLYGI"
     gene            2717..3076
                     /locus_tag="PLE88_12470"
     CDS             2717..3076
                     /locus_tag="PLE88_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77319.1"
                     /translation="MSEHAFILYGSYQNPYLRAVRISDGLIFDVAAVQLAQAPAWEDT
                     VISLGSKDAVINGWPVDLPESLPNGTYDLQLYDAAAPSQSDAMIAGWRLVMPHKLLVN
                     PTEFPLDVFGRIRTTHA"
     gene            3069..3491
                     /locus_tag="PLE88_12475"
     CDS             3069..3491
                     /locus_tag="PLE88_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77320.1"
                     /translation="MLKITLLFFDSAKVVRSVDKSTRKVLSKFGAFVRRTAKQSIRKR
                     KKASAPGTPPSSHTGLLKRFIFFGYDTQKRSVVIGPTRLTENNRGEAPSILEYGGRTT
                     VKSSKKKTRVRIRSRPFMGPAFEKEKTNLPTLWKNSIK"
     gene            3504..3953
                     /locus_tag="PLE88_12480"
     CDS             3504..3953
                     /locus_tag="PLE88_12480"
                     /inference="COORDINATES: protein motif:HMM:NF017961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HOM77321.1"
                     /translation="MADFLLGMNAKIYQGAAASDPTTLDPSTLTEMGNVKDVTLSLEA
                     GEADITTRANSGWRATAPTLRECSCEFEMVWKPGDAGFEAIKSAFLSAGTVALAVLTG
                     AHNESGAEGPVGNWSITNFSRSEALEEAVTVSVTAKLAAFGQWFEAA"
     gene            3979..>4223
                     /locus_tag="PLE88_12485"
     CDS             3979..>4223
                     /locus_tag="PLE88_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77322.1"
                     /translation="MKTFNDAAGRTWTIALNLGTAMQVKDKLNIDLLRPEDGDPPLLT
                     QLGTDEMLLGEVLCALLGPQFEKHNVTDADVRMAFDGS"
BASE COUNT         1092 a         1013 c         1092 g         1026 t
ORIGIN      
        1 atgggatgga ccaggccatt cgcggtaacg gcaagccggg tattcaactc agactggacc
       61 ggctcgagca ggaaagaatc tcccgcagca aggtgacgtg gtttttgttg ggaattgctg
      121 caagcgtggg cggtgccgta ctgacatctt tgatactggg gtggctatga agctggcttt
      181 agatatcgca gatgcgattg ttaccgaact ggctggcaac aacttcagtg aaccgttggt
      241 ggtaacgcgt cgggtgctgc cggaatacga gatcgccgaa ctgaaagcat tgacctttac
      301 ggtcgttccc aaatccgttg agattatcaa tatcacgcgg cagtccagca gctttgatgt
      361 ggccatcgac atcggcattc agcagaaaat cggcaaggac accgatgcgg aagcgacccg
      421 cttaagcggt atcgttaccg aggttgtcag ttttctgaat cggcgcaagt tggctggttt
      481 ccctgctgcg gtttttgttt cgattgccaa tgagccggtt tacgcaccgg aacatctgtc
      541 tgaaaaaagg ctttttacct ctattctgac cctaacttac aaggtgattg atggctaatc
      601 aaattgaaca actgacaacc tggatggtca accgtgctaa tcaacaggga ttatccattc
      661 agcagtttaa ggacatcaag tttcagcatg ttcgcagcaa tttacctgct gcctaccggg
      721 accggttaac gcaagcggtc tacataaaag ccaaacggct ggcactgact caattgttcc
      781 aggaaaaact ggaccagctc aagaacaata caaatgtccg gcagaccatt ttgacagttt
      841 ttcctgatgc gacgtttcgt gtttatccga ataagcggca aatcattatc gaggttcagt
      901 aatgacccaa atctatcata aaaaagcatg ttgctggttc tataaatcag gcagcggaag
      961 caatcaggct ggtggatgca cgaaagaatt cctggataac cacaacggtg atgtctcact
     1021 ggtcatgggt ccagccggtc aacccaaaca aaacgcgaat gcagacgcaa tcaacgattt
     1081 taataccgaa accgctcaat taactgccag cgggctcgat actaatgtac tggccggact
     1141 agttgcacga atcaacaatt cattctgtga agttgtgagt gttggccttg atacggttgg
     1201 tattgaccag tgggccggga aaagcgagta catctggacg gatacgaatg aaacaaattc
     1261 tatcatctac cccgatgatg acagcatcta ctcggtcggt ggttatatcc atatcaaagg
     1321 aacgaacggt caactcgccg acggcaccta tgaaatcctc tcgatggaat atggtggtct
     1381 tgaaattgac gatggcatgg ccgtttttga caatgattgg ggttatcttt attgtgcacc
     1441 tgaatccatg tccggtgtta atattgtcat cgggggcgcc tataatgatc ttcaggatgt
     1501 gctgaatatc cccgatgcgg tctatcacga ccaatggatt ttctttaatg aggaaatcgc
     1561 accgtcagtt ttggttaacg gatcggccta ttatatttat tcgagccatg atggcgaagc
     1621 cgccaacaac accaagctct atatcgtcgg atacaacact tacgcttatg attgcctgcc
     1681 tgatgggtat ggttatttcc ctgaggggac tcaaaatgtc agcacacact ataaaacagc
     1741 attagagcgt gccaagaacc cctcggatac atcgattaaa gcagacctgc cgacagcaac
     1801 cacgaaaaat attacagata actggggtcg catcttccgt gtgtacacca gcgctgaaaa
     1861 cgttcagttt atgggtatct attttgaagt ggctgtctat cattggccga taatggccga
     1921 caacgaaaca actggttcgg tgtttgtgaa acattgtgct gggggccata tcagttacga
     1981 tgcagcacca gccttttacg gaggccaaat cgttcgggtt aatgatgccg cgaccggagg
     2041 cggaattttt gacagcttct tcaagggggt ggaaatgttt aacggcacca ccccgacggt
     2101 cgatagttcc ggtgattggg agtttgccta taacgtgggg atttacactt ccaatatcaa
     2161 tcccgattat tccggccctg tggttcacca caatatcttt gtcaaaagca actggggtgt
     2221 ctcggcagtc gcacgctcct tccagaatgt gttcaacaat atcttctatc tgtgccgtca
     2281 ggccgggctg cacttaaacg gtactgaagc acggatgcgg gcctggaaca atatcatcgt
     2341 tatggatgaa accagcggta ttttcgatgg gctcttccgc atcagtgcag gcggcacggt
     2401 cgattatctt gattacaact gttactgcaa ccagaatggt cagcctgttt caatatttgc
     2461 attcgatacg gaagcgaatc catcttttaa cttcgacaat ctcaaaaaag gcccccatga
     2521 cattgaagct gatccgatgt tcatggaccc cgataactgg gattttcggc tgcgagagaa
     2581 tagtccctgt attggcatgg gccgaccgga tctgcagggc aatcccgcaa ccatcggtcc
     2641 gtatctggaa aaacagaccg ttggcctcta tggaaaaacg aaaagcctct atggtatcta
     2701 aggaaggaat cttttcatga gtgaacatgc atttattttg tacggaagtt atcaaaatcc
     2761 ttacctgcga gcggttcgga tttcggatgg gcttattttt gatgttgctg cggttcagtt
     2821 ggctcaggca cccgcctggg aagataccgt gatttcatta gggagcaaag acgccgtcat
     2881 taatggctgg ccggtggatt taccggagag cctgcccaat gggacttatg accttcagct
     2941 ctacgatgcc gctgcaccat cccagtctga tgcgatgatt gccgggtggc ggctggtgat
     3001 gccacataaa ctgctggtca atcccacaga atttccgctg gatgtgttcg gtcggatcag
     3061 gacaacccat gcttaagatt acactcttat tctttgacag tgccaaggtt gtccggtcgg
     3121 tggataaatc gactcggaag gtgctctcca agttcggagc ctttgttcgg cgaacggcca
     3181 aacaaagtat ccgcaaacga aagaaagcat ccgctccggg tacaccccca tccagtcata
     3241 cgggactttt gaagcggttt atcttcttcg gatatgacac gcaaaaacgc agtgtcgtca
     3301 ttggtccaac gcggctaacc gagaacaacc gaggcgaggc accgagcatc ttagaatacg
     3361 gcggccggac aaccgtcaag agcagcaaga aaaaaacacg tgtacgaatc cggtctcgac
     3421 cgttcatggg accggccttc gaaaaagaaa aaaccaacct tcccactctg tggaaaaact
     3481 caatcaaata ggagaacacg tctatggcag attttttatt gggcatgaat gctaagattt
     3541 atcagggcgc agcggcgtct gatccaacga ctttggaccc ctctacgttg accgagatgg
     3601 gcaacgttaa ggatgtgacg ctgtcgctgg aagcaggcga agcggacatc accacccgtg
     3661 ccaacagcgg ttggcgggca accgcaccga ccctgcggga atgcagttgt gaatttgaga
     3721 tggtctggaa gcccggtgat gcgggatttg aagcgatcaa gtcggcattc ctgtcggcag
     3781 gaaccgtagc tttagcggtt ttaaccggtg cacacaatga atccggtgcc gaaggtccgg
     3841 tgggtaactg gtccatcacc aacttctcgc gaagtgaagc actggaagag gccgtcaccg
     3901 tcagcgtcac cgccaaattg gccgcattcg gtcagtggtt cgaggcagca taaaactctt
     3961 tagcgaaagg acactctgat gaaaacattt aatgatgccg ccggacgaac ctggacgatt
     4021 gccctgaacc tgggaaccgc catgcaggtt aaagacaaac tgaacatcga cctcttgcga
     4081 cccgaagatg gcgatccgcc gctgctgacg cagttgggca ccgacgagat gctgttgggt
     4141 gaggtcttgt gcgccttgct cggtccgcag tttgaaaaac acaatgttac cgatgcagat
     4201 gtgcggatgg cgtttgacgg ttc
//
LOCUS       DAOTBO010000170         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_17322_length_4171_cov_6.101312, whole genome shotgun sequence.
ACCESSION   DAOTBO010000170 DAOTBO010000000
VERSION     DAOTBO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17322_length_4171_cov_6.101312"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..531)
                     /locus_tag="PLE88_12490"
     CDS             complement(64..531)
                     /locus_tag="PLE88_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77323.1"
                     /translation="MKQITAILLLSGLCILSSCTKEEDTAKDLARMKNCRELVFLHND
                     DYHGKPKTVFAKITEQEKIDNILLAMKNSEPYAPALPPLLVYSIRGVGKNGATKQVKF
                     TWSDDAKAVLFSDSRSKDAYQLFVEYSIIDPSAPVIIGYPDLKEGEELEHVKN"
     gene            complement(543..3905)
                     /locus_tag="PLE88_12495"
     CDS             complement(543..3905)
                     /locus_tag="PLE88_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77324.1"
                     /translation="MKAAFLILCTGAVVCTVSAGTISLVADTTLPDPNDEITIWVQTD
                     EPLMCLGLAVYVTGDAAITTAMSEADCAAFGWDNGWNSDPYIDPNGWIYLSGVRWVSD
                     ANDIVSYFKFRYYSGQVSVYIDQENSIGFSWDGNTVTYSTLSIETLLFGEPTESQLQE
                     SDSEETAIDDDPVPNVSAPKPKSLEECISRTAYFNDEKTTSSVESLNEFANHKQRRET
                     AFLEQSISLSTEQSQNEAVSELRETLLESPESVIEVSPGNVPTVWTKDNTYHVTGTVV
                     LPPACYFEPGVHVSRAPNSRMVSDSVFPPIMCGTPEEKIWMYPDADPYYYGHYVSGIE
                     YTDLAGTLEMRHVIIEGAYYGVIFENCTFNNDIHDLYLFNNVYGIYADGNEDLKFSLI
                     LAVGNYYTGIEVYLEAGCDIKITQSTIDYSPWDGIIIAGTVQATDCTAIVANTAITRC
                     GLGPNPYAYGVQTFGYLYAAIYGMGYYGNSNDNPAGFIEYYDVHAPSLPYESNGTLLG
                     SHYLKKTSPFYNGGVGYVTETPQILICTTSTDNTPLTGVLPIGFCYAIDPNFTNEGLG
                     YVLETDVDNSKGVDFEDFAAISKYWLLDLDPNSFDPNSVDPNDIDPHVCDFDGNKIID
                     IGDLIKLSEEWLQFGSGVPQLALLPDNDPNGNGQYTILSNSIEFSLVNQMEGYDYYLF
                     RDGHYISKFKGNVDLAETSYASDDTFKAVNGQHRYRVAACSRNERLTVVSKAMPYELS
                     NTLHGVVSSKVYDPNHPYIISGFNNSAELLIFKLFNMEEAVVWDANIPSGSFSLTIDP
                     NVFYTPLVTVPAMINPYCTYLFTNPEIQMLMQGATSFEDAPGWSSTIVEKFNIRTWRN
                     RGTKSLLIAPNRGIFRARATSNHAWCQAMRNRNLVPITGLFGDAATREAQDFMFNDPS
                     LRSIRINTHGAFQIRAGGRTVRRTVYELGDGTLCFSWIASANQNPPNPADFLPGDYEQ
                     TGYSIRSARNWSALPYLYVDADFCASGRARMPDAYYKDICDAPEGSGYYNDDDFWNGM
                     DMARWAFDIRPMGETRRRIYLGWYTDSGDNMLGLTGLNNWYMAFYASWGKETNPQPYY
                     GAHSHATTSTTRGPEVHRQKTYCGNSNSYLSN"
BASE COUNT         1104 a          970 c          914 g         1183 t
ORIGIN      
        1 taacggccat tgtagcacgt gtgaggccct ggacataaag gccatgagga cttgagtaaa
       61 ctctcaattc ttcacatgtt ctaattcttc gccttcctta agatcaggat atccgattat
      121 cactggtgca gaagggtcga taatactgta ctccacaaaa agctgatagg catccttaga
      181 tcggctatcg ctaaataaga ccgcctttgc atcatccgac caagtgaatt taacctgctt
      241 tgttgcaccg tttttcccaa cgcctctgat agaatataca agcagaggcg gcaatgcggg
      301 tgcataaggt tcagaatttt tcatggcaag aagtatgtta tctatttttt cctgttccgt
      361 aattttagca aaaacggttt tcggtttgcc atggtaatcg tcgttatgta gaaataccag
      421 ttctcggcag tttttcattc gagccaaatc ttttgccgta tcttcttctt tggtacatga
      481 acttagaata cataagcccg acaacaataa aatagctgtt atttgcttca tcgctgttcc
      541 ctctaattac tcaggtaact gtttgaatta ccgcaatatg tcttttgccg atggacttcc
      601 gggccgcggg ttgttgaagt tgtcgcgtga gaatgggcgc cataatacgg ctgaggattt
      661 gtttcttttc cccaacttgc ataaaaggcc atataccaat tattgagtcc tgtaagcccc
      721 aacatgttgt cccctgaatc ggtgtaccat ccaagatata tccggcgtct ggtttcaccc
      781 attggcctaa tatcaaaggc ccatcgagcc atatccatcc cgttccagaa atcatcatca
      841 ttgtaataac cggaaccttc cggcgcatca cagatgtctt tatagtatgc gtcaggcatt
      901 ctggcacgtc cgcttgcgca aaaatccgca tccacataaa ggtacggcag agcactccag
      961 ttacgagcgc ttctaatgct gtaccctgtt tgttcataat caccggggag gaaatcggcg
     1021 ggatttggcg gattctgatt tgctgaagcg atccatgaaa agcatagtgt tccgtctccg
     1081 agttcatata ctgttcttct gacagtacga ccacctgcac gaatctgaaa tgctccgtgg
     1141 gtgtttattc ggattgaacg cagagaggga tcgttgaaca taaagtcctg cgcttcacgg
     1201 gttgcggcat caccgaacaa tcctgtaatc gggacaagat tcctgttgcg catggcctga
     1261 caccatgcat gatttgaagt cgctctggcc ctgaatatgc ctctgttagg cgctataagc
     1321 aaagacttag tgcctctgtt tcgccatgtt ctgatattga acttctcaac aatagtgcta
     1381 ctccaaccgg gcgcatcttc aaaactggtg gctccctgca taagcatttg gatttcaggg
     1441 tttgtgaaaa gataggtaca gtatgggttt atcattgccg gaacagtgac aagtggcgta
     1501 tagaacacat tcgggtcaat tgtaaggctg aaactgcctg acgggatatt tgcgtcccaa
     1561 acaaccgcct cttccatatt aaacagcttg aaaattaaaa gttcggcgct gttgttaaat
     1621 cccgatataa tgtacggatg attcgggtca tagactttgc ttgatacaac tccgtgtaat
     1681 gtatttgaca actcataagg catagccttg ctgacaacgg tcaatctttc gtttcgactg
     1741 cacgccgcaa cacgataccg atgctggccg ttgactgcct tgaacgtatc atcgctcgca
     1801 taactggttt cagcaagatc gacattgccc ttgaacttac tgatataatg accgtctctg
     1861 aaaagataat agtcgtatcc ttccatctga ttgaccaatg aaaattcaat gctatttgag
     1921 agaatcgtat attgcccgtt gccgttgggg tcattatctg ggagcaaagc caactgcgga
     1981 acacccgacc cgaattgcag ccattcctct gacagcttga tgagatcacc aatatcaatt
     2041 atcttgttgc cgtcaaaatc acagacatga gggtcaatat cgtttggatc tacgctgttc
     2101 gggtcaaagc tattcgggtc taaatcgagt aaccagtact tgctgattgc cgcgaagtcc
     2161 tcaaaatcaa cacctttact gttgtcgaca tccgtttcaa gtacatagcc caatccttcg
     2221 tttgtaaaat ttgggtcgat agcataacaa aatccgattg gcaggacacc ggttaaaggg
     2281 gtgttgtctg tacttgttgt gcatatcaaa atctgcggcg tttcagtgac gtatccaacg
     2341 ccgccattgt aaaagggact ggttttcttg agatagtgac tgcctaacaa tgttccgttt
     2401 gattcatagg gcagggatgg agcgtgtaca tcgtaatact cgataaaccc tgccggattg
     2461 tcattactgt ttccataata gcccatccca tatatcgcgg catacagata cccaaatgtc
     2521 tgaacaccgt atgcgtaagg atttggcccg agcccgcatc gcgtgattgc agtattagca
     2581 actatcgcag tgcaatctgt cgcttggacg gttcctgcaa tgataattcc atcccacggg
     2641 gaataatcga tggttgattg ggttatcttg atgtcgcagc ctgcttcgag ataaacttca
     2701 atgcccgtat agtaatttcc gaccgcaaga ataagagaga atttaaggtc ctcattgccg
     2761 tcggcgtata ttccatagac gttgttgaaa aggtataaat catgtatgtc gttattgaac
     2821 gtgcaattct caaaaatgac tccgtaataa gcgccctcta taataacgtg tctcatctca
     2881 agtgtacctg ccaagtcggt gtattcaatg ccggaaacat aatgtccgta gtaatacgga
     2941 tcggcatcgg gatacatcca aattttttct tccggcgtac cgcacatgat tggcggaaaa
     3001 actgagtcgc tgaccatacg tgagtttggt gctctggaaa catggacgcc cggttcaaaa
     3061 taacaggcgg gcggcagcac gaccgtacca gtcacatgat aggtgttatc ctttgtccag
     3121 actgtcggta cattgccggg acttacttca attaccgatt ccggcgattc caagagtgtt
     3181 tcacgaagtt cagaaacagc ttcattttgt gattgctctg ttgacaaaga aatgctttgt
     3241 tccagaaaag ctgtttcacg tctttgtttg tgatttgcaa actcgtttaa agattcgacc
     3301 gatgaagttg ttttttcatc attgaaatag gcagtccgtg agatgcattc ctcaagtgat
     3361 tttggctttg gcgcggaaac attggggaca gggtcatcat caatagccgt ttcctcggaa
     3421 tcggattctt gtaactgtga ttctgtaggc tctccgaaca aaagcgtttc aattgaaaga
     3481 gttgagtatg taacagtatt tccgtcccaa ctgaaaccta tgctgttctc ttgatcgata
     3541 taaacggaaa cctgtccgct gtaatagcgg aatttgaagt agctgacaat atcgttggcg
     3601 tcagataccc agcggacgcc gcttaagtaa atccaaccgt ttggatcgat gtaggggtca
     3661 gaattccaac cgttgtccca accgaaagcg gcacaatcgg cttcactcat ggccgttgta
     3721 atcgccgcat ctccagttac ataaacggca agacccaggc acatcaatgg ctcatcggtc
     3781 tgaacccaga tagtgatttc atcgttcgga tcgggtaatg tcgtatccgc aaccaatgag
     3841 attgtccccg ccgatactgt acaaacaacc gcccccgtgc acaaaatcaa aaacgctgcc
     3901 ttcatagttt tccctttcct gcttaaaata ccctcgttcc ttatggtttt caaccttttt
     3961 tcggttcttc caaatgccgg actggcaaaa aataagcaga ccagtccggc gaccgctata
     4021 gagcggcaga tataatatat tatcggcctt attaaacaat tagcaaaaag caggaaatat
     4081 acccactctg atttatcgcc tataaaatcg gctttaatag ggatttttta tggttcagcg
     4141 cacaaaaaaa ggccctgctt tgtgagcagg g
//
LOCUS       DAOTBO010000171         4080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_17802_length_4080_cov_4.710062, whole genome shotgun sequence.
ACCESSION   DAOTBO010000171 DAOTBO010000000
VERSION     DAOTBO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17802_length_4080_cov_4.710062"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLE88_12500"
     CDS             <1..383
                     /locus_tag="PLE88_12500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77325.1"
                     /translation="KIGIRKQVIFAGNRKDVPQLLMFIFDLLLFPSIYEGMPLTLVEA
                     TAAGVPIVCSDVICQEATNVCPEHIVRLPLSVGPEIWAKHTLATLENKVDFSKEAFDK
                     MSKSHFSDQYSLEQLMKIYNQAVH"
     gene            395..1636
                     /locus_tag="PLE88_12505"
     CDS             395..1636
                     /locus_tag="PLE88_12505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77326.1"
                     /translation="MRLMIATENRFYEGPSGRIYSDTVCNYQFWRRYLQVFDEVVIFA
                     RVGKIDKEPTKPAADGAGVSFYRLPMYVGPQQYLMRRKQIHEIAARATEHADAFILRI
                     PGRCGTLLWKELRKRSIPFGVEVVGHAGDSCKTSGANFFVKNMLRFVSPRQQRLECRQ
                     AAAAAYVTERYLQKDFPPGGWSTYYSSIDLYEDAFITEAQLREKLARLGEAFEGKRPF
                     RLCHAGTMAAKYKGQDILIEAAGRCLREGLNLEVVLMGDGRFRPFYEQKAAAMGIAER
                     VRFLGSVPPGQAVRDEYDRADMLVFPSLTEGLPRTIIEAMARGLPCAASNVGGIPELL
                     AEEFLVKPGDADDLARRIAFMLSRREVLERAALQNLHKAREYRYDVLNERRISFYEQV
                     RQASLTFNSRDKGKGRIYGNE"
     gene            1788..2669
                     /locus_tag="PLE88_12510"
     CDS             1788..2669
                     /locus_tag="PLE88_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77327.1"
                     /translation="MEAVRNLQKYHYWAGDFGRKELYYQRNFVFGMKLRMVVKDAFGE
                     RPEIAVNKHYLKHIKWRFNNLHSPGYILTDLVCVLLLHRQMCAIHCSAFTLGGKTVLV
                     VAPPDTGKTLTTMRAVFDYGASYISEDLAITDGRKIYACPWTSTFRYYDELSMSRMMA
                     LRMKLIKIIPVVEIMPFPGKTRTIDTYITHERIVEEAPIDYVAVLARREGGIKVLDKD
                     EALEMMYNLNRYEFCYRKSPMLTAYTYFNPEINLDEMAARERRILGNIVSRSQCLLVQ
                     DTDPRTFAKQIVEFIQG"
     gene            2744..3922
                     /locus_tag="PLE88_12515"
     CDS             2744..3922
                     /locus_tag="PLE88_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM77328.1"
                     /translation="MVLNDSTKMCFVSTVPNTLYFYAELLKALRQRGVLMTVVSSESP
                     LLFEMQKQFDCEVYPVNITRTISPLKDIQSCRALCRLLKNRKYDLVHAHTPKGGLLGM
                     AASRWANVPVRLYTIHGLPLETARGFKRTLLKKADQVACSCATHVLAVSPSLRDRVLE
                     ERLCKAAKLSMLGDGSACGVNLARYTRTADIIAKAASVRKQLQIPQDALVLGFVGRLT
                     PEKGIPFLVKNSLRLFEKYENLHLLIVGGIDNSREVLEEDVLHAMKTHPRIHMAGHIR
                     FPVEYYAAMDILVLPTKREGFGMCNIEASAMGLPVVASRIPGCVDSVQEGRTGFLYAL
                     NNDDEFTAALEDLIEHPEKRKEMGEAGAQWVRQCFSSERLVEEHIRFYEKVLNSKRRV
                     "
     gene            complement(3899..>4080)
                     /locus_tag="PLE88_12520"
     CDS             complement(3899..>4080)
                     /locus_tag="PLE88_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77329.1"
                     /translation="FRPIKIKNHGLKHKNHQQKLKQFVFQQSHLSPNSSDLRSSISPA
                     VRRPHRFASYSSFTV"
BASE COUNT         1013 a          793 c         1148 g         1126 t
ORIGIN      
        1 aaaaaatagg catcaggaaa caagttattt ttgcggggaa tcgtaaagat gtgccgcagc
       61 tccttatgtt tatatttgat ctgttgctgt ttccttcaat ttatgaagga atgcccctta
      121 cgttagttga agcgactgcg gcaggggtac ctatcgtgtg ttctgatgtt atttgtcaag
      181 aagcaacgaa tgtttgcccc gaacatattg tgagattgcc attgtctgtt ggtcctgaaa
      241 tatgggcaaa gcatactttg gctactttag aaaacaaagt tgacttttcc aaagaagcat
      301 ttgataagat gagtaaaagt catttttcag atcagtattc attagaacaa ttaatgaaaa
      361 tttataacca agctgttcac tgaaagccat tccaatgcgt ctcatgattg ctactgaaaa
      421 tcgtttttat gaggggccga gcggtcggat ttattcggat acggtttgta attatcaatt
      481 ttggcggcgt tatttgcagg tttttgatga ggttgtgatt tttgcccgcg ttgggaagat
      541 tgataaggag ccaaccaagc cggcggccga cggtgccggg gtaagttttt atcggctgcc
      601 gatgtatgtg ggcccgcagc agtatctgat gcggcgcaag caaatccatg aaatagcggc
      661 tcgtgcgact gaacacgctg atgctttcat ccttcgcatt ccgggacgct gcggaacgct
      721 gctgtggaaa gagcttcgca agcggtctat tccattcggg gtggaagtgg tcggccatgc
      781 aggcgattcg tgcaagacaa gcggggccaa tttttttgtg aaaaacatgc tgaggtttgt
      841 cagtccgagg cagcagcgtc tggagtgccg gcaagcagcg gcggcggcgt atgtaacaga
      901 gcgctatctt cagaaggatt ttccgccggg cggctggagc acctattaca gcagtatcga
      961 tctttatgaa gatgctttca taacagaagc ccaactgagg gaaaaacttg ctcgtttggg
     1021 ggaggcgttt gagggcaagc gtcccttccg cctctgccat gccggcacaa tggctgccaa
     1081 atataaaggg caggatatcc tgattgaggc ggcggggcgg tgtttgcggg aggggctgaa
     1141 tcttgaagtt gtgctgatgg gcgacgggcg gtttcgtccg ttttatgaac agaaggcggc
     1201 ggcgatgggg attgcggagc gtgtccggtt tctgggctct gtgccgcccg ggcaggcggt
     1261 gagggatgaa tatgaccggg cggatatgct ggtgtttccg tcgctgacgg aagggctgcc
     1321 tcggacgatt attgaagcga tggcgagggg tctgccgtgt gcggcttcta atgtcggcgg
     1381 gataccggag ctgctggcgg aagagttttt ggtcaaaccc ggcgatgcgg acgatttggc
     1441 gcgacgaatt gcatttatgc tttcccgaag agaggttttg gagagggccg cattgcagaa
     1501 tcttcataag gcccgcgaat atcgatatga tgtcctgaat gagaggcgga tttcttttta
     1561 tgagcaggtc cggcaggcca gtttgacttt caacagccgt gataaaggaa aagggcgaat
     1621 ttatggaaat gaataattac gcagtctgta aagatgttct gggcatacag accaattcga
     1681 aatacatcgg atggtctttt gggcaaatag ttcagccggt tgatccgtct cagctggaca
     1741 aatgccgggt cgttgttcgt ttgagggtgg acgatcttca gcaagaaatg gaagccgttc
     1801 gcaacttgca gaaatatcat tattgggcgg gcgacttcgg tcgaaaagaa ttatattatc
     1861 agcgtaattt tgtgtttgga atgaaattgc ggatggttgt caaggatgct tttggtgaac
     1921 ggcctgagat tgcggttaat aagcattatt tgaagcatat caagtggcga ttcaataatc
     1981 tgcattcgcc gggttatatt ctaacggatt tggtttgcgt attgctgctg catcggcaga
     2041 tgtgtgcgat tcattgttcg gcgtttactc ttggaggaaa aaccgttctt gtagtcgcgc
     2101 ctcccgatac cggaaagacg ttgaccacga tgcgtgcggt attcgattat ggggcatctt
     2161 acatttctga agatttggcc atcacggacg gccggaaaat atatgcctgt ccgtggactt
     2221 caacattccg ttattatgat gaactcagta tgagccggat gatggcactt cgaatgaaac
     2281 tgattaaaat catcccagta gttgaaatta tgccttttcc gggcaaaacc agaaccattg
     2341 acacctatat tactcacgaa cgaattgttg aagaggcccc gatcgattat gtggctgtct
     2401 tggcccgtcg ggaaggcgga ataaaggttc ttgataaaga tgaagcgctg gagatgatgt
     2461 acaacttaaa cagatatgaa ttttgctatc ggaaaagccc gatgttaaca gcctacactt
     2521 attttaatcc tgaaattaat cttgatgaga tggctgctcg tgaaagaagg atcctcggaa
     2581 atatagtgag tcggtcacaa tgtttgcttg tgcaggatac tgacccgcga acttttgcca
     2641 aacaaattgt tgaatttata caaggttaat gcttatacaa ttcctgccta tttgcagcca
     2701 agccgatttg tctgtttcaa agctccgtat agaaaggtaa ctcatggtat tgaatgacag
     2761 cactaagatg tgttttgtat ctactgtccc aaataccttg tatttttatg ctgaactgct
     2821 gaaggcactg cgccagcgcg gagtcttgat gacagtggtt tcttctgagt cgcccctttt
     2881 gtttgagatg caaaagcagt ttgattgtga agtatatcct gtgaatataa cccgaactat
     2941 cagcccgtta aaggatatac aatcctgccg ggctttgtgc cggctgctca agaaccgaaa
     3001 atatgatctg gtccatgccc atactcctaa aggcggcctg cttgggatgg ctgcttctcg
     3061 atgggctaat gtgccggttc ggctttatac gattcatggg cttccgttgg aaacagcaag
     3121 gggtttcaaa cgaacattgc tgaaaaaggc cgatcaggtt gcctgttcct gtgcaactca
     3181 tgtgttggca gtaagcccaa gtcttaggga tcgggttctt gaagagagat tgtgtaaggc
     3241 cgcaaaatta tctatgttgg gagacggcag tgcgtgcggg gtgaatcttg cgcgttatac
     3301 gcgtaccgct gatattattg caaaggcagc atctgttcgg aaacaattgc aaattccgca
     3361 ggatgctttg gtattgggtt ttgtaggccg cctgactccg gaaaaaggca ttccttttct
     3421 ggtaaaaaac agccttcgtt tatttgaaaa gtatgaaaat cttcatttat tgattgtagg
     3481 cggaattgat aattcaagag aggtattgga agaggatgtt ttgcatgcta tgaaaaccca
     3541 tccacgtatt catatggcgg gtcatatccg atttccggtc gagtattatg cggccatgga
     3601 tattttggtg ttgcctacga aacgggaagg gttcgggatg tgcaacattg aagcgtcggc
     3661 catggggctg cctgtggtgg cttctcggat acctggatgt gtggattcgg tgcaggaggg
     3721 ccggaccggc tttctgtacg cattgaataa cgacgatgaa tttacggctg cgttggagga
     3781 cctaatcgaa catcccgaga aaagaaagga aatgggagag gcaggggcac agtgggttcg
     3841 gcaatgtttt tcttcggaac gtttggttga ggagcatata cgcttctatg aaaaagtctt
     3901 aaacagtaaa cgaagagtat gaggcgaaac gatgcggacg gcggacggcg ggactgatgc
     3961 tggagcgaag gtctgaggag ttgggcgaaa gatggctctg ctgaaaaaca aactgtttca
     4021 acttctgctg gtgattcttg tgcttcaggc cgtgattctt tatcttaatc ggcctaaaga
//
LOCUS       DAOTBO010000172         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_17991_length_4043_cov_5.618355, whole genome shotgun sequence.
ACCESSION   DAOTBO010000172 DAOTBO010000000
VERSION     DAOTBO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17991_length_4043_cov_5.618355"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PLE88_12525"
     CDS             complement(<1..1218)
                     /locus_tag="PLE88_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77330.1"
                     /translation="MRKYLIILLGTVFAATLQGATPFILIDNFSDASLSEYTKTVILD
                     QNAGHTAVFQSPAGTLQLTGGANGVEQLLLLRDDYSLGVNQMLIADLAWGQVARNDIG
                     IAVAAVKNPAGLADGATGDVREDYIAVYVQADNTNLKGIFVNGKTAGSTIYAGGLPAD
                     PKNHVTGLYIRRNSLTEFTLGFIVDRTTYTDFATASITNTNIGKAVGFFGDFRNVAVF
                     GDLDNLRIESETFGPHDPSVEQMANPVSGKVNAALGWKAGADPAGIYAVNPAIAEEYL
                     FMSKNASDPNLYYIGSAGAPSIENPSSSFTVENLLYDSTYRWAVAEALTGYTQTFTKG
                     ISTLGDVDPNNIIGPVWTFDTLLSIPVIQQDLTDVRVFENESAAAFTVVFTSVNPVTV
                     SWYKNGSLLTAGGD"
     gene            complement(1260..2981)
                     /locus_tag="PLE88_12530"
     CDS             complement(1260..2981)
                     /locus_tag="PLE88_12530"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM77331.1"
                     /translation="MDAKQKQELNELLYLTLERSAQPWQTEKLNAILQSSPEALQYSL
                     DFYLMSSALRKTNAIPHAFFGSKNEITEQFNLLHCLAVEERTAPSIKLSADKPNTHQP
                     IIPAQPLEKKVRKINRFSLAVAAVSLAALLCMIAYVQVVPSREPAAVLIDAMDIKVKN
                     TFKTLKKGDIFYNTDRPIALEQGIVNIQFGYGADVIIEAPCEFACKSNGTLELKSGKA
                     FASVPPEAIGFIIETPSSRMIDLGTEFGVEVEPCGSSSINVFQGQTQLVSRSPSKQVK
                     SVILTKNQSRKIDIQTGTIEECAFHERTFIQYFNSETKTILTRKYLCLADIVGGGNGT
                     GNGQLNKGVDVLTGTVKLFTEAGGSQPGTREYLAVRENPFIDGVFVPDGGSGPVVVSS
                     QGHRFENCPDTNGQFWGGIFNGGWHESTVSAIPRHPLRLDGIVYGTGIQPSIYIHANQ
                     GITFDLNALRSGFPKLAIYRFTALCGISDTLSDYADQLRAWKDNWTNLMPECSLADVY
                     VLVDGQVRFAKKDLSVADAAAAIAVDIYPEDRFLTLVTTQGSDPWGNTNDWTLIAQPR
                     LLLTELK"
     gene            complement(2995..3501)
                     /locus_tag="PLE88_12535"
     CDS             complement(2995..3501)
                     /locus_tag="PLE88_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77332.1"
                     /translation="MEIKDSHEFLQLLLINQMRIYAFILSLVRNYEDADDLMQETANT
                     MWQKYPDCKPIKDFVSWGIQIAYYKILDFRKHQKNNSHILLDNELFEKIVPIVQQAHT
                     KVDSRIEKLKKCLQRLSEREYKLIELRYYQNFKPQQIASVLGLSILNTYKSLSRVHGR
                     LLECMRQA"
     gene            3762..>4043
                     /locus_tag="PLE88_12540"
     CDS             3762..>4043
                     /locus_tag="PLE88_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77333.1"
                     /translation="MQNDKSEQFLSLFAKNQKRIYGFITVLVPQAVDADDLMQETMLV
                     MWRRFNEFKMGTDFAAWGISVARRIVLKFYSERKNKALLFDDETMQAILQ"
BASE COUNT         1086 a          916 c          901 g         1140 t
ORIGIN      
        1 atcccctccg gcagtcagaa gagaaccgtt tttataccaa ctgacagtta cagggttgac
       61 actggtaaac acgacagtga aggcggcggc cgattcgttt tcaaaaacac gcacatctgt
      121 caaatcctgc tgtatgaccg gaatagacag aagcgtatca aatgtccaca cagggccaat
      181 gatgttgttg ggatccacat cgccaagagt gctgatgcct tttgtaaatg tttgcgtata
      241 gccggtcagt gcttctgcta cagcccaccg gtaagtgctg tcatacagaa gattttcaac
      301 agtaaatgat gaacttggat tttctatgct gggggcgcct gcggagccga tataatacaa
      361 gttcgggtcc gatgcattct tggacataaa gaggtattcc tctgcaatag caggattgac
      421 agcgtaaatg ccggccggat ctgctcctgc cttccatccc agcgcagcgt tcaccttgcc
      481 gctgacagga ttcgccatct gttcaacgga tggatcgtgg ggaccgaagg tttcgctttc
      541 tattcgcaga ttgtccagat cgccgaatac tgcaacattc cgaaaatccc caaaaaagcc
      601 cacggccttt ccgatattcg tattagtaat ggaagcggtt gcaaaatccg tgtaggttgt
      661 gcggtccaca ataaaaccca gcgtaaattc cgttaagctg ttgcggcgaa tatacagacc
      721 ggtaacgtga tttttgggat ctgcaggaag gccgccggca taaatcgtac ttcctgcggt
      781 tttgccgttt acaaaaattc ccttcaaatt ggtgttgtca gcttgcacat aaacggcaat
      841 ataatcttcc cgcacatcac cggttgcgcc gtcagccagt cccgccggat tttttacagc
      901 agctacagca atcccaatat cgttgcgggc aacctgaccc catgccagat ccgctatcag
      961 catctgattg acgcccaatg aataatcatc ccgcagcaag agaagttgtt ccaccccgtt
     1021 tgcccctccc gtcagctgca gtgtgccggc gggtgattga aacacagctg tatgtccggc
     1081 gttctgatcc aaaatgactg ttttggtgta ttcggacaga ctcgcgtcgc tgaaattgtc
     1141 tatcagaata aaaggcgttg ccccctgaag ggttgcagca aatactgttc ccagcaaaat
     1201 tatcagatat ttcctcatat cacaatctcc gaactattga tccttgcttg atttaatgtt
     1261 tattttaact cggtcagcag gagccggggc tgggcaatga gagtccagtc attggtattg
     1321 ccccacgggt cgctgccttg cgtggtcacc agtgttaaaa aacggtcttc aggatatatg
     1381 tcaactgcaa ttgcagctgc ggcatccgct acagaaagat cttttttggc aaaacgcact
     1441 tgcccgtcca ccagcacata cacatctgcc agcgaacatt cgggcatcag gttcgtccag
     1501 ttatccttcc acgctcgaag ctggtcagca taatcgctca gtgtatccga tatgccgcaa
     1561 agggcggtaa atctataaat cgctaacttc ggaaagccgc tgcgcaatgc attcaggtca
     1621 aatgtgatac cttgattggc atggatataa atggaaggct ggatgccggt gccgtaaacg
     1681 atgccatcca atcgcagcgg atgtctggga atagcggaaa ctgtggattc atgccaaccc
     1741 ccgttgaaaa ttcctcccca gaattgacca ttggtatcag gacagttttc aaaccgatgt
     1801 ccctgactgg atacaactac cgggccgctg cctccatccg gcacaaaaac accgtcgata
     1861 aagggatttt cccgaacagc cagatactct cttgtccccg gctgagaacc gccggcttcc
     1921 gtaaacaatt ttacagtacc ggtcagtaca tcgactcctt tattgagctg cccatttcca
     1981 gtaccgtttc ctccgccgac aatatcagcc aaacaaaggt attttcgggt taaaattgtt
     2041 ttagtttcag aattgaaata ttggatgaaa gtccgttcat ggaacgcaca ttcttcaatc
     2101 gttcccgttt gaatatcaat ttttctgctt tgattcttgg taaggatgac ggatttaact
     2161 tgttttgacg gcgatcttga aacaagttgt gtttgtccct gaaaaacatt gatagaagaa
     2221 gaaccgcacg gttctacctc caccccaaac tccgtaccca gatcaatcat gcgggacgat
     2281 ggtgtttcaa ttataaatcc gatagcttcc gggggtaccg atgcaaatgc tttgccggat
     2341 ttcagttcaa gagtgccatt ggacttgcag gcaaattcac aaggcgcttc gattatgaca
     2401 tcggctccat acccgaactg gatattgaca attccttgtt ccagggcaat aggcctgtcg
     2461 gtattgtaaa agatatcgcc ttttttaagc gttttgaagg tatttttgac tttgatgtcc
     2521 atcgcatcaa tgaggacagc ggctggttcg cgtgaaggta caacctgcac ataggcgatc
     2581 atgcacaata gggcagccag cgatacagca gcaacagcca gtgaaaagcg gttgattttt
     2641 cttacttttt tctccagcgg ctgtgcagga ataattggct gatgcgtatt tggtttgtcg
     2701 gcggaaagct ttatagaagg agcggttcgt tcttcaaccg caagacaatg aagcaggttg
     2761 aattgctctg ttatttcatt ttttgagcca aagaaagcat ggggtatagc attagtcttt
     2821 cgcagtgcag aagacattag ataaaagtcc agactgtatt gcagcgcctc aggagaggac
     2881 tggaggatgg cattgagttt ttcggtctgc caaggctggg ccgatcgctc caaagtgaga
     2941 tataacaatt catttaattc ttgtttttgt tttgcatcca ttatatctca aatcctatgc
     3001 ctgacgcatg cactccagaa gccgtccgtg aacacgcgac agacttttgt aggtattcaa
     3061 tatcgacaag ccgagaactg aggcaatttg ctgcggctta aaattctgat aatatcggag
     3121 ctcgataagc ttgtattctc tttcactaag tctttgaagg cactttttta atttttcaat
     3181 ccggctgtca acctttgtgt gggcctgctg aacaattgga actatttttt caaacaattc
     3241 gttatcgagc agaatatgag aattattttt ttgatgtttt cggaaatcca aaatcttgta
     3301 ataagcgatc tgaattcccc aagacacaaa atctttaatc ggcttacaat ccggatattt
     3361 ttgccacatt gtatttgcgg tttcctgcat taaatcatcg gcatcttcgt aattgcgaac
     3421 cagacttaag ataaaagcat aaatacgcat ctggttaatt aaaagcagct gcaggaactc
     3481 atgagaatct tttatttcca tccatcactc cgattagctc tcattggccg tttaacttta
     3541 agtatgttga atgtctatgt aaaattgaga cataaaaggc agaattttaa taagaaaata
     3601 ctataaaaag tttgtttgat ccagaatgtg ctaattcaga tttatattgt cttgattggg
     3661 aacttcgata aataatctta ttatggtaag gatttttttc taaaattcca accaatttag
     3721 cattacctat gcaaatattg gagaatttgc ggtaatagaa gatgcagaac gataaaagcg
     3781 aacaatttct atctttattt gcgaaaaacc agaaacgcat ctatggtttc attacagtgc
     3841 tggtgccgca agctgtagac gccgacgatt taatgcagga gacgatgctt gtgatgtggc
     3901 gtcgttttaa tgagttcaag atgggtactg attttgccgc ttggggtatt tctgttgctc
     3961 ggcggattgt tttgaaattt tactccgaac gaaagaacaa agcactgctc tttgatgacg
     4021 aaaccatgca ggcaatcctt cag
//
LOCUS       DAOTBO010000173         3998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_18227_length_3998_cov_5.291656, whole genome shotgun sequence.
ACCESSION   DAOTBO010000173 DAOTBO010000000
VERSION     DAOTBO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18227_length_3998_cov_5.291656"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /gene="hemC"
                     /locus_tag="PLE88_12545"
     CDS             <1..749
                     /gene="hemC"
                     /locus_tag="PLE88_12545"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOM77334.1"
                     /translation="ALLDGRADLAVHSLKDLPTAPTKGLTIAAVPPRESACDVIAAQK
                     NLDSLDDLPVGAVVGTSSLRRIAQLKAMRPDVDCRPLRGNIETRLKKVLSGQMTAAVV
                     AQAGLNRLGLEKAVSYILPADRFLPAPGQGALAIQVRDDNRRLMDAVSVLDDARTRIA
                     TQTERRILAGLHGGCSIPLGAYCSIENGQACIRAVLLNPDGTGLIRKEQICPAETALH
                     AADMIVAQILQAGGKEILDRLSRQNQNDCR"
     gene            774..1751
                     /gene="hemB"
                     /locus_tag="PLE88_12550"
     CDS             774..1751
                     /gene="hemB"
                     /locus_tag="PLE88_12550"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOM77335.1"
                     /translation="MSYPAARLRRLRTSPSMRRLVRETSLCTDNFIYPLFVRRGSKIK
                     EPIDSMKDCFHLSPDTAAHEAVQAAAVGIGAVLLFGLADKKDSAGSEAYRADSAVCES
                     IKRIKDAVPDMVVCTDVCLCAYTDHGHCGLIEDGRIANDKTCQLLGRMALAHAKAGAD
                     MVAPSDMMDGRVSVIRQMLDADGFTDTAIMAYSAKYASAFYGPFRQAADSAPAFGDRK
                     TYQMDPADSRQALREIEMDIQEGADIVMVKPALAYLDVIRIAKDRFDVPLAAYNVSGE
                     YMMLNAAAEAGLLDRTQAMLETLISIKRAGADIIITYFAKEAARILHNA"
     gene            2358..3032
                     /gene="lexA"
                     /locus_tag="PLE88_12555"
     CDS             2358..3032
                     /gene="lexA"
                     /locus_tag="PLE88_12555"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOM77336.1"
                     /translation="MSPHIPITPRQLHTLRQVKHLAQKQCYSATIGELAAALHISRTT
                     AFEHVAALREKGLLEHSTGRARCLTLTPAGETLLERAQRPKTPKQKPTLQPYAAKTHS
                     ESIFLCGRVSAGYGIDAIEEKEPFSLDDVFGSRQQLFVLRVCGQSMIGAGIHDGDYII
                     CRSAQTAENGQIVVAMLDGQNATLKRFYKDRKTARLLPANDAFEPIYSDSCQIQAVAV
                     GIIRRF"
     gene            complement(3029..3574)
                     /locus_tag="PLE88_12560"
     CDS             complement(3029..3574)
                     /locus_tag="PLE88_12560"
                     /inference="COORDINATES: protein motif:HMM:NF022389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HOM77337.1"
                     /translation="METPKSHLAVSELNFSLFQRPLHPELFTIYAQRRIKTENYETLI
                     WATGGSHVVTLITKDACLTELICTPDQCLPKRGLIERFQFRGQRSHKCTLSRGLSYMT
                     DFQVEKMSANLYRQSHYDLERFARNRGIFVPHPELAVGGLEPFTYIDFEARRDELHIH
                     AFHAFPDQVTIIKTQSLFDLH"
     gene            complement(3747..>3998)
                     /locus_tag="PLE88_12565"
     CDS             complement(3747..>3998)
                     /locus_tag="PLE88_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77338.1"
                     /translation="IILSVAISAVLFSMHHHFFFANGHFYQGDTFTVGKFVFRAMAGV
                     YFAVLYAVRGFGITAGTHVFYNVLAALVRTLIFMIQPDS"
BASE COUNT         1038 a         1016 c         1038 g          906 t
ORIGIN      
        1 aagccctctt agacggcaga gccgatctgg ctgtgcattc gctgaaagac ctgcctactg
       61 ccccaaccaa aggactgacc atcgcggctg ttccgccgcg cgagtcggcc tgcgatgtca
      121 tcgctgcaca aaagaatctg gactcgctgg atgatttgcc tgttggggca gttgtcggga
      181 catccagtct tcgccgaatc gcccagctta aagcaatgcg tccggatgtg gactgccggc
      241 ccctgcgggg caatatcgaa acccgtttga agaaggtcct gtcaggacaa atgacggctg
      301 ccgtcgtggc acaggcaggg ctgaaccggc tgggtttgga gaaagccgtt tcgtatatcc
      361 tgccggccga caggtttctg cctgcaccgg gacagggggc tttggcaata caagtgcgcg
      421 atgacaaccg ccggctgatg gatgccgtat cggtgctgga tgacgcccgg actcggattg
      481 caacccagac cgagcggcgg attctggccg gtctgcacgg cggatgcagc atcccgctgg
      541 gggcttattg ttcaattgag aacggacagg catgcatccg ggccgttctg ttgaatccgg
      601 acggaacagg cctgattcga aaagagcaga tctgtccggc cgagacggct ttgcatgccg
      661 ctgatatgat agtcgcccag attctgcagg caggcggaaa agagatactc gaccggctga
      721 gcagacaaaa ccagaatgat tgccgataat ggcatagaaa ggacaaaacc aaaatgagtt
      781 atcctgcagc acggcttcgg cggcttcgga caagcccctc gatgaggcgg ctcgttcgcg
      841 aaacatcgct ttgtacagac aatttcattt atccgctgtt tgtacgcagg ggcagcaaga
      901 tcaaggaacc gattgattcc atgaaagact gttttcatct gtctcctgac actgcggccc
      961 acgaagcagt gcaggccgcc gccgtcggca tcggtgctgt tctgctgttc gggctggcag
     1021 acaagaaaga ttctgccggc tccgaggcat accgggccga cagcgctgtc tgcgaatcaa
     1081 tcaaacgcat caaagacgct gtaccggata tggttgtctg caccgatgtc tgcctgtgcg
     1141 cctataccga ccacggacac tgcggtctga ttgaagacgg tagaatcgcc aatgacaaga
     1201 cctgccagct tttaggccga atggctcttg ctcacgccaa ggcgggagcg gatatggtgg
     1261 ctccttcgga tatgatggac ggccgcgtca gcgtcatccg tcaaatgctc gatgccgacg
     1321 gctttacaga cacggctatt atggcctatt cggccaaata tgcatcggcc ttctacggac
     1381 cgtttcgcca agcagccgac agcgcaccgg cattcggcga ccgcaaaaca taccagatgg
     1441 atccggccga cagccgacag gctctgcggg aaatcgagat ggatatccaa gagggagccg
     1501 atatcgtaat ggtcaaaccg gcattggcct atttggatgt gatacgaatc gccaaagacc
     1561 gcttcgatgt accccttgcg gcttataatg tctccggcga atatatgatg ctgaatgccg
     1621 ccgccgaagc cggcctgctc gacagaacac aagcaatgct cgaaacactt atttccataa
     1681 aacgcgccgg agcggatatt atcattacct attttgccaa agaggccgcg cgaatccttc
     1741 acaatgcata agcggatttt cggtgctgtt tacacaaacc tgaagccgca tcttttgcca
     1801 tttatgctat aactacatcc gcattatcgc cattacggtt aatgatagtc ttccggaata
     1861 attttcctcc cgaaacaata tgctctggcc ataacttatt attaaaacag cggttatatt
     1921 ttgtccgaaa aaatactggt cttgtattga aaaatttgtt cggtttttgt ttgggttttt
     1981 aagccctctt tgccgatata tcctaacgcg gaagttgcag agacaatcaa acatttgcag
     2041 ataaaaaaag tttgaagatg ctcttttgca gaaataaaaa taaattacag caaggaacgc
     2101 aggaatgaat cggtttgaaa aaacaaaaca ggatatccct ttatgcaaat ttgggccggg
     2161 cggcgattat gtcaattcct ggtctgcccc ggaatcgccg atgccgcagg aaaggcattc
     2221 gccgctggga aaagttctga atcttatcgc ggaaatcctc ggcgccgctg tacagccgga
     2281 aattttgacg gaaattgcat gcgccctgcc gacggaaccg ctgaatctgc aaactgcatc
     2341 tcgggagaaa acagagaatg agcccgcata ttcccatcac accccgccag cttcatacgc
     2401 tgcggcaggt aaaacatctt gcacagaagc aatgttattc ggcgacgatt ggggaactgg
     2461 ctgcggccct gcacatcagc cggaccaccg cgttcgagca cgtcgccgca cttcgcgaaa
     2521 aaggccttct ggaacattca acaggcaggg cacgctgttt gacacttacg ccggccggcg
     2581 aaaccctgct cgagcgggct cagcggccta aaactcctaa acaaaaacca acccttcagc
     2641 cctatgccgc taagacacac agcgaaagta tttttctttg cggtcgcgta tccgccggat
     2701 atgggataga tgccattgaa gaaaaagaac ctttttcgct ggatgatgtg ttcggcagcc
     2761 gccaacagtt atttgtgctt cgggtttgcg gccagagcat gattggagcc ggcattcacg
     2821 atggggatta tatcatttgc cgaagcgccc aaacagctga aaacggccaa attgtcgtag
     2881 ctatgctgga cggacagaat gccaccctta aacgctttta caaggacaga aaaactgctc
     2941 gcctcctgcc ggccaatgac gcttttgagc cgatttattc ggattcgtgc caaattcagg
     3001 ctgtcgctgt cgggattatc cgccggtttt aatgcaggtc aaaaagagac tgggttttaa
     3061 taatcgtcac ctgatcggga aaagcatgaa atgcatggat atgcagctcg tcccgacggg
     3121 cctcaaaatc aatataggta aacggctcca ggccgccgac agccaattcc gggtgcggga
     3181 caaaaatccc ccgattgcgg gcaaaccgtt caaggtcata atgactctgc cgatagagat
     3241 tggcactcat cttttcaact tgaaaatcgg tcatgtagct gagcccgcgg cttagtgtgc
     3301 atttgtgact gcgctgaccg cgaaactgaa accgctcaat caaaccccgc ttgggcagac
     3361 actgatccgg tgtgcatatc agttctgtca ggcaggcatc tttggttatc agggttacca
     3421 cgtgggagcc gcctgtggcc caaatcaacg tctcataatt ttctgttttt atgcgccgct
     3481 gggcataaat cgtaaaaagt tctgggtgaa gcgggcgctg aaaaagggag aagtttaatt
     3541 cgctgacggc taaatggctt ttgggtgttt ccattggttc tctcacacat agacatcatt
     3601 gctgtcgtat tacagctgtt attatatgaa aatagactgt ctgaatcaag gccattttca
     3661 gtattgatag gcaagtttgt gcggcttatc ggacggtgat tttgcaagta taagaagtca
     3721 aagacattag caaataaaaa aattttttat gaatcgggct gaatcataaa aatcagtgtg
     3781 cggactagtg ccgccaaaac attgtagaaa acatgtgtgc cggccgttat gccgaacccc
     3841 cgcacagcat aaagaacggc aaaatagacc cccgccatgg cccgaaagac aaatttgcca
     3901 acggtaaatg tatcgccctg ataaaaatgg ccgttggcaa aaaagaaatg gtgatgcatg
     3961 ctgaaaagaa cagcagaaat ggccaccgac agtataat
//
LOCUS       DAOTBO010000174         3972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_18365_length_3972_cov_4.862139, whole genome shotgun sequence.
ACCESSION   DAOTBO010000174 DAOTBO010000000
VERSION     DAOTBO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18365_length_3972_cov_4.862139"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="surE"
                     /locus_tag="PLE88_12570"
     CDS             complement(<1..563)
                     /gene="surE"
                     /locus_tag="PLE88_12570"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOM77339.1"
                     /translation="MHILLTNDDGIFAPGLAAIYRRLTTLGEVTVAAPSDIQSGAGHS
                     ISLKEIRGEYIRIAGKFDGYSISGSPADCVKLAIHKLIDARKPVDLVVSGLNHGANVG
                     INVFYSGTVAGAIEAAFYNLPAIAVSAAFDEPMDFEAASDAALSVIRQLMDLPRGCVA
                     NLNIPRLSAGTPKGILVVPQSTHGFDEG"
     gene            1043..2581
                     /locus_tag="PLE88_12575"
     CDS             1043..2581
                     /locus_tag="PLE88_12575"
                     /inference="COORDINATES: protein motif:HMM:NF017561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77340.1"
                     /translation="MKTLMYSMLCVAVIAGLAGCTCPGAAPAPAPAPAPKPAPAPAPA
                     PAPQAAACGNYTVSQDYMQSGAIRIEKVMPAMVQLNAPFEYTIKVTNLTDMTLADVEI
                     RERIPASLKNISSSPAGKMEGGTAVWKMDTLGPRAVEKIVVKGSASETGCLQTCANAT
                     YTILACANTQVVQPALALTKTAPASVTICDPIPLQFTVSNKGSGTATNVKLTDALPAG
                     LTTQDGKTSIELALGNLAPGQSVSRTVVAKAAKTGTYKNQAAAVADGDLKAQSETTTT
                     VVTQPVLAIQKTGPKKIYIGRSMEYEITVANKGDSAANQVVLTDTVPAGVTGVKVSDG
                     GTVAGSQITWNIGTLAPNASKKVTVSYSPTGGGEFCNTTKAVATCAEAVTAQACTTVE
                     GIPAVLLEVIDVSDPIEVGGNETYIITVTNQGSAQDTNISIKAMLEESMQYVSSSGAT
                     SGSFADGTVTFAPLPNLAPKAKATWQVVVKAVKPGDVRFKVVMNTDQLSRSVEETEAT
                     HFYQ"
     gene            2662..3078
                     /gene="mscL"
                     /locus_tag="PLE88_12580"
     CDS             2662..3078
                     /gene="mscL"
                     /locus_tag="PLE88_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOM77341.1"
                     /translation="MSFLSEFKAFAMRGNVVDMAVGIVIGGAFGKIVSSLVNDIIMPP
                     IGLLLGGVDFSNLKIILKKAAVDAAGNAVPAVSINYGQWINTVIDFLIVAFAIFMVIK
                     GMNALKKKEEAAPSAPPAPTKEQILLTEIRDALRSR"
     gene            complement(3182..3691)
                     /locus_tag="PLE88_12585"
     CDS             complement(3182..3691)
                     /locus_tag="PLE88_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77342.1"
                     /translation="MKRSVVILIVAVLAVVGCGKKEKPAPPAEPQSVPGMLDSTKQTA
                     DKAVEKTTQAAKDAAAAVKESFSTEINLDKTIADLKAEAAKMDLPALIETAKKYKAAI
                     LQKQDAMKALMDKLAALGLAEKAGPQGQSLTAEIKALTDAAAGLKERLNVYIDTIKAK
                     GGDASDLAM"
     gene            complement(3750..>3972)
                     /locus_tag="PLE88_12590"
     CDS             complement(3750..>3972)
                     /locus_tag="PLE88_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOM77343.1"
                     /translation="DLIQKITQLNRLAQQRGQTLAQMALAWVLSRKTVTSALIGTSRL
                     EQLLDNLKTLENLCFSEEQLRQIEAVLNA"
BASE COUNT          987 a          983 c         1015 g          987 t
ORIGIN      
        1 ccctcatcaa aaccgtgggt tgattgcggc acaactaaaa tgccttttgg cgtaccggcc
       61 gatagacggg ggatgttcag gttggctaca cagccgcggg gcaggtccat cagctggcga
      121 ataacggata gtgccgcatc ggaagccgcc tcgaaatcca ttggttcatc gaaggcagcg
      181 ctgacggcaa tggccggcag gttgtaaaag gctgcttcaa tcgctccggc tacagtgccc
      241 gagtaaaaca cattaatgcc cacattggct ccgtgattca ggccggaaac gaccaaatcg
      301 accggtttac gggcatcaat gagtttatgg atagccagtt tgacgcagtc agcaggggag
      361 ccgctgatgc tgtagccgtc gaatttgccg gcaatgcgga tatattcacc gcgtatctct
      421 ttcagtgaaa tgctgtgccc agcccccgac tggatatccg atggagcagc aacggttacc
      481 tcccccagag tggtcaaccg cctgtaaatg gccgccaaac ccggcgcaaa aataccatca
      541 tcattggtca ataaaatatg catcgtattg ttcttgtctg aactatcggt catcttaaca
      601 tatcgggttg aacagcaatt gtcaaacgaa agacaacgaa attcctgctc caatctgcgc
      661 tgcggaagaa tggtgatatc cgcggatggt caaattgaga ggctggcttt ccggttcttc
      721 ctgtgcctga caacaatctg ctgcaggcag tgcggcccga ttcgtgatgc gtacgctttc
      781 aaacactccc aaaccgagcc agcggatatt tattagcgga ttaaaagata aaaaatattt
      841 tttaaaaagt agttttttta ttcaaatttt gtataattag aattaaaaaa ggcagaatgg
      901 attttaactt ttacgcaaag gaaaactctt gtgaatgctg atgtagtgga aaataaaagc
      961 ccgccgtgac acgattttga ccttctatgt ttttagggag aaaaggtatt cctgccaatt
     1021 taatttaatt aaggagtgtt ctatgaaaac gttaatgtat tctatgttgt gtgttgccgt
     1081 cattgcagga cttgcaggat gcacttgtcc gggggcagcc ccggctccag caccggcacc
     1141 tgccccgaaa ccggctcctg ccccggctcc agcaccggct cctcaggcag cagcctgcgg
     1201 caactatacg gtgtctcagg attatatgca gtctggtgca atacgcattg aaaaagtaat
     1261 gccggcaatg gttcagctga atgccccgtt tgaatacacc atcaaggtga ccaatctgac
     1321 ggatatgact cttgccgatg ttgaaatccg ggaacggatt cctgccagtc tgaagaatat
     1381 cagttcatct ccggcaggaa aaatggaagg cggcacagcg gtgtggaaaa tggatacatt
     1441 aggacctcgg gccgtcgaga agattgtcgt gaagggttct gcttctgaga ccggatgtct
     1501 gcaaacgtgt gcgaatgcca cctatactat tttggcctgt gccaatacgc aggttgtcca
     1561 gccggctttg gcgctgacca agacagcacc ggccagtgtt acgatctgcg atccgattcc
     1621 gctgcagttt actgtttcca acaaaggaag cggaacagct accaatgtta agctgacgga
     1681 tgcgctgccg gccggcttga caacacagga cggcaagaca agcattgaac tggctttggg
     1741 gaatctggca ccgggacagt cggtatcgcg aacggttgtt gccaaagcgg ccaagaccgg
     1801 aacctacaag aatcaggccg cagccgttgc tgacggtgat ttgaaggccc agtcggaaac
     1861 gaccacaacg gttgtaaccc agccggttct ggctatccaa aagaccggtc ccaagaagat
     1921 atacattggc cgttctatgg agtatgaaat tacggttgcc aacaagggtg acagcgcagc
     1981 caaccaagtg gttttgaccg atacggtgcc ggcaggcgta acaggtgtaa aggtcagcga
     2041 cggcggcacg gtggccggca gccagattac gtggaatatc gggactctgg ctccgaatgc
     2101 cagcaagaaa gttaccgtct cctacagccc gacaggcggc ggcgaattct gcaatactac
     2161 caaagctgtg gcgacctgtg ccgaagcagt tacagcacag gcctgcacga ctgttgaggg
     2221 cataccggct gtcctgctgg aagttatcga cgtctctgac cccattgagg tcggcggcaa
     2281 cgaaacctac attatcactg taaccaatca gggttcggct caggacacca atatctccat
     2341 caaggcgatg ctggaagaat cgatgcagta tgtcagttcg tccggtgcca caagcggaag
     2401 ctttgcagac ggaaccgtga catttgctcc gctgccgaat ttggctccga aggccaaagc
     2461 aacgtggcag gtggtggtca aggccgtcaa gccgggtgat gttcggttta aggtggtcat
     2521 gaataccgac cagctcagca ggagcgtcga agagacagaa gccactcatt tctatcagta
     2581 ataaagaagt aacagcaaaa accggccgca ggcattttgc ctgcggcttt tttaaatcta
     2641 ttttttagaa gggagtcgcc tatgtcattc ttgtcggaat ttaaagcttt tgcgatgcgg
     2701 ggcaatgtcg tcgatatggc tgtgggtatt gttatcggcg gtgcgttcgg caagattgta
     2761 tcgtctctgg taaacgatat tattatgccg ccgattggac tgctgctggg cggcgtggat
     2821 ttttcaaatc tgaagataat tctcaagaag gcagcagtcg atgcagccgg caatgctgtt
     2881 ccggcggtgt ccatcaatta cggtcagtgg attaatacgg ttattgactt tctgattgtg
     2941 gcttttgcca tttttatggt gattaaaggg atgaatgccc ttaagaaaaa agaagaggcc
     3001 gcaccttctg cacccccggc tccaacaaaa gaacagattc tgctgaccga aatcagagat
     3061 gctctgcggt cccggtaata ccggtaatat tggaaccagc acaatacgcc ggaaacaaac
     3121 acaacaaaaa aggtccgccc taaagcgagc ggaccttttt tactttgcct tcaccccaag
     3181 cttacatcgc taaatccgat gcatcaccgc cttttgcttt gatcgtatca atatagacat
     3241 tcaaacgctc ctttagtccg gcagcagcat ccgtcaaggc ctttatttcc gctgttaaac
     3301 tctggccctg cggacctgct ttttctgcca gcccaagggc cgccagctta tccatcagcg
     3361 ccttcattgc atcctgcttt tgcagaattg cagccttgta ttttttggcc gtctcaatca
     3421 gtgccggcaa atccatttta gccgcttccg ctttcaggtc agcaatcgtt ttatccagat
     3481 taatctctgt tgagaaagac tctttgacag ctgcagcggc atctttggcc gcttgggttg
     3541 ttttctcaac cgctttgtcc gctgtctgct tggtgctgtc cagcatcccc ggcacacttt
     3601 gtggttcagc cggcggggca ggcttttcct tttttccgca gccaaccaca gcaagaaccg
     3661 ctactatcaa aatgacaacc gatcgtttca tagcattctc cttaaaaaat tgtcttgttt
     3721 tgcggccgca tgtttattca gattggaatc tacgcgttta aaacggcttc aatctgccgg
     3781 agttgttctt ctgaaaagca aagattctca agagtcttga gattatccag caattgttcg
     3841 agccggctgg tgccgataag agcactggtt acggtttttc ggctaagtac ccaagcaaga
     3901 gccatttgag ccagtgtttg accccgctgc tgtgccagtc tattgagttg ggttattttt
     3961 tggatcaggt cc
//
LOCUS       DAOTBO010000175         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_18856_length_3890_cov_3.344459, whole genome shotgun sequence.
ACCESSION   DAOTBO010000175 DAOTBO010000000
VERSION     DAOTBO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18856_length_3890_cov_3.344459"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..395
                     /locus_tag="PLE88_12595"
     CDS             9..395
                     /locus_tag="PLE88_12595"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HOM77344.1"
                     /translation="MVEAFAGKYPIVADPGPDVARIRIAITNIKPSKPGLSAVTSIVP
                     VGLAVSTVKRGATGGWAGSGETCAEMMVLDSSTNDLILMAADQQQAAFASRFSKFGSA
                     KDAFKFWSERIVGFIDNAKGIDRSNP"
     gene            678..1541
                     /locus_tag="PLE88_12600"
     CDS             678..1541
                     /locus_tag="PLE88_12600"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOM77345.1"
                     /translation="MTLVKRYLLRFGFLTTALIVVLLLMQVSPLQADSPKISEQEAYD
                     IGVEAYVYLYPLITMDVTRKVMTNVPAGVKEGVGPMGVFNHFRAFPTADFRDVVRPNF
                     DTLYSIAWLDLTKEPMILSAPETDGRYYLLPMLDMWSDVFAVPGKRTSGTSAVNFALV
                     PKGWHGKLPKGVERIDAPTPYVWIVGRTQTNGPEDYKAVHKVQNGYWITPLSQWGKKA
                     KPIKFVADPSVDMKTPPVTQVNTMPAAEYFSYGAELMKVNPPNITDWSQIARMKRIGL
                     EPGKPCNKSRS"
     gene            1570..2088
                     /locus_tag="PLE88_12605"
     CDS             1570..2088
                     /locus_tag="PLE88_12605"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOM77346.1"
                     /translation="MQDKIPTLARVVNGWQMNTDTMGVYGNYYLKRAIVVLIGLGANQ
                     PEDAVYPLNITDAGGNPMEGTNKYVIHFTKETIPPVNAFWSITMYDEEGFQVSNRINR
                     FAIGDRDKLKFNTDGSLDIYIQSDSPGADRESNWLPSPAKGKLGVTMRLYAPKAQVLD
                     GGWNPPVVRRVD"
     gene            2184..2372
                     /locus_tag="PLE88_12610"
     CDS             2184..2372
                     /locus_tag="PLE88_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77347.1"
                     /translation="MNGRQTIILIAALVVSALIVWHDLPIEFPWMINVVKLFLKLSVV
                     LGFAVFAFVFTGGKKKAS"
     gene            2626..3468
                     /locus_tag="PLE88_12615"
     CDS             2626..3468
                     /locus_tag="PLE88_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77348.1"
                     /translation="MILMKSLKNRINHFHWSTLCVIALFPLIMLCPQIAMAAESQEEL
                     AKKSLNPVAALISLPLQFNQDYDIGLKDDAKRTWLNIQPVIPVSLNQNWNVIIRTIVP
                     LIDMDSPIPGGDDESGLGDITQSFFLSPKDPIDGWIMGAGPVLYYPSATDETLGSEKW
                     GAGPTVVLLKQESGFTVGLLANHIWSFAGDDEREDLSATFLQPFVGYTTKTLTTLMIN
                     TESTYDWEADQWTVPINVMLNQMFKIGPQPLSLQFGYRYYAEKPQYGPDWGIRFTVTF
                     LFPK"
     gene            3551..>3890
                     /locus_tag="PLE88_12620"
     CDS             3551..>3890
                     /locus_tag="PLE88_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOM77349.1"
                     /translation="MRQTRWIKFLGSFLLASLLFNGPAFAGGISLYEFGSPDIGLAAA
                     GWAARAQDAATVFTNPAGMSRLEQSQFLGGLQALYGDLKFDANNNTTYTGSDGGNAVG
                     WVSGGSLFVVQ"
BASE COUNT          975 a          957 c         1032 g          926 t
ORIGIN      
        1 accaggcgtt ggttgaagcc ttcgccggaa agtaccccat cgtggcagac cctggccccg
       61 atgtcgccag gattcgcatc gccatcacaa acattaagcc aagcaagccg ggcctcagtg
      121 ccgtgacctc aatagtacct gttgggttgg ccgtcagcac agtcaagcga ggagccacgg
      181 gaggctgggc cggcagcggg gaaacgtgtg ccgaaatgat ggtcctcgac tcatcgacca
      241 atgacctaat tctaatggcg gcagaccagc agcaggcagc ttttgcatca cgcttttcca
      301 aattcgggtc ggcaaaagat gccttcaagt tctggtctga acggattgtg ggattcatcg
      361 ataacgcgaa gggaattgac cgaagcaatc catagtgctg acaaatcggg gcaataaaac
      421 cctttctcag gcttgaagct atggaatcaa gtttgagatg tgcccatgtt gatagggaat
      481 tcgatgtaca ccgcatgtgg tgcattgcaa aaagactttg ggtaagggaa ggatgttttg
      541 agtcctaacg ctgtttacat gactcttgga ttcattgcga gtggttttgg gggaattcaa
      601 cagcctccga gccaccctcc cagtacttgc cgcccatagc ggtaatatga gtaattttct
      661 gaagattgga gctgaaaatg acacttgtta aaagatacct gttgagattt ggtttcctca
      721 ccacggcact tattgttgtt ctgctgctga tgcaagtttc tcctctccaa gcggatagtc
      781 ctaaaataag cgagcaggag gcctacgata taggtgtcga agcgtacgtc tatctatacc
      841 cactcatcac gatggacgtc acccggaagg ttatgaccaa tgttccggcc ggagtgaaag
      901 agggcgtagg gccgatggga gtattcaacc acttccgagc ctttcctacc gctgatttcc
      961 gtgatgtagt gcgtcccaac tttgacacgc tttattcaat cgcctggctt gatctgacca
     1021 aagaaccgat gatcttatcg gctccggaga cagacggacg ctactatctc ctgcctatgc
     1081 tggacatgtg gtcggatgtc tttgcggtgc cgggcaagcg cacgagtggc acgagcgctg
     1141 tgaacttcgc tcttgttcca aaagggtggc atggaaaatt gcctaaaggt gtggagcgta
     1201 tcgatgctcc tacaccctac gtatggatcg tcggtcgcac tcagaccaac ggccctgaag
     1261 actataaagc ggtccataaa gtacagaacg gatactggat cacccccctt tcccagtggg
     1321 gcaagaaggc caagccgata aagttcgttg ccgatccaag tgtcgatatg aagacgccgc
     1381 cggttacgca ggtgaacacc atgcccgcag ccgaatattt cagttatggc gcggaactaa
     1441 tgaaggtcaa tccgccgaat ataaccgact ggtcacagat cgcacgtatg aagcggatcg
     1501 gtctggaacc cggcaaaccg tgtaataaga gccgctcttg agtgtgtccc ggcggatgct
     1561 ctgaaaatca tgcaggacaa gattcctact ctggcacgtg tggtcaacgg ttggcagatg
     1621 aatacggaca ccatgggggt ctatgggaac tactacctga agcgtgcgat cgttgtgcta
     1681 atcggtctgg gtgccaatca gcccgaagat gcagtgtatc ctctcaacat aaccgatgcc
     1741 ggcgggaatc ctatggaagg tacgaataaa tacgttatcc acttcaccaa agagaccatc
     1801 ccccccgtga atgccttctg gtcgatcacg atgtacgacg aggaggggtt ccaggtgtcc
     1861 aatagaatca accgtttcgc tatcggcgat cgtgacaaac taaaattcaa tacggatggg
     1921 tcattggata tctatatcca gagcgattca cccggtgcag acagggaatc caactggcta
     1981 ccgtcacctg ccaagggcaa gctcggagta accatgcggc tgtacgcacc caaggcccag
     2041 gtgcttgatg ggggctggaa cccgccagtg gtcaggcgcg tcgattgaca gaacctgtga
     2101 tggagattct gaaaactgtt attgccgcag caagcttcct gaagctgtgc agcagacaga
     2161 gatcatcaac caggaggcaa gatatgaacg gaagacaaac catcatcctg attgccgctc
     2221 ttgtagtgtc cgcattgatt gtctggcatg atctgccgat tgaatttccg tggatgatca
     2281 atgtggtcaa gctattctta aagttgtccg tcgtattggg attcgccgtc tttgcctttg
     2341 tctttaccgg aggaaagaaa aaagcatcat aaaagaggga gtgcggcgta ttgcctgcct
     2401 gccgaagcag tggtccaatg catcgctttt ttaaaaaaac tggaacgacg aaacaggggt
     2461 tcactactcc tgtaagaaat ataaagtttt ctccagttta gcaaatatct gcgggccatg
     2521 acgcaatttc cacaaacggg cctcctgctt ccctggtaag ggctacgatc aaagcactta
     2581 gcttgcgata cggagggatg atctgaatat caaaaggatc ttatattgat acttatgaaa
     2641 tcactgaaga acaggattaa tcatttccat tggagcacac tttgtgtcat tgctctgttt
     2701 cccctgatca tgctttgtcc gcagatagcc atggctgcgg aaagtcagga agagctggcc
     2761 aagaagtctt taaatccggt agcggccctg atcagcttac cgctgcagtt caaccaggat
     2821 tacgatattg gattgaaaga tgatgcgaaa cgcacctggc tgaacatcca gccggtcatc
     2881 cctgtttcac ttaatcagaa ctggaatgtg atcatccgga ccattgtccc gctcatcgat
     2941 atggattcgc ccatccccgg cggcgatgat gagagcggcc tgggtgacat cacacagagc
     3001 ttctttttat cgcccaagga tcccatcgat ggatggatca tgggggccgg tccggtcttg
     3061 tattatccgt cggcgaccga tgaaacgctc gggagcgaaa agtggggtgc cggaccaacg
     3121 gttgttctgt tgaagcagga aagcggattt actgtcggcc tgctggccaa ccatatctgg
     3181 tcttttgccg gggatgacga acgtgaagac ctcagcgcaa cttttctcca accttttgtc
     3241 ggatacacga ccaaaacatt aaccacgctg atgatcaata cggagtccac ctatgattgg
     3301 gaagccgacc aatggaccgt acccatcaat gtcatgctga accagatgtt caagatcgga
     3361 cctcagccgc tcagtcttca gtttggctac cgctactatg ccgagaaacc gcaatatggg
     3421 cctgattggg gcatccgttt taccgtcacc tttctgtttc ccaaataata cggaagggat
     3481 gaggaagatg cagccttgtt attaaatatt taattaatta aatgtgttaa tgagtaatgg
     3541 agggatttga atgcgacaga cgaggtggat aaaattcctt ggctcatttt tactggcaag
     3601 tctcttgttc aacggacctg cctttgccgg cgggatctca ctctatgagt tcggctcccc
     3661 ggacataggc ttggccgccg ccggttgggc tgcccgtgca caggatgcgg cgacggtctt
     3721 taccaacccg gcgggaatga gccgcctgga gcagtcacaa ttcctgggcg ggttgcaggc
     3781 actttatgga gatctaaaat ttgacgccaa taacaacacg acctacacgg gaagcgatgg
     3841 cggaaatgct gtgggctggg tgtccggggg cagtcttttc gtcgtccaga
//
LOCUS       DAOTBO010000176         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_19060_length_3856_cov_4.937911, whole genome shotgun sequence.
ACCESSION   DAOTBO010000176 DAOTBO010000000
VERSION     DAOTBO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19060_length_3856_cov_4.937911"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLE88_12625"
     CDS             <1..200
                     /locus_tag="PLE88_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77350.1"
                     /translation="NYGLSAETIGGQYYGVTGIRPCIDPAFAGNAANFDNTGSSYCKV
                     DLADLALLASKWLNNGLYTAP"
     gene            302..3739
                     /locus_tag="PLE88_12630"
     CDS             302..3739
                     /locus_tag="PLE88_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM77351.1"
                     /translation="MNLTVDHKTSVVTHRTAFYTCNRRLSHAAVYVMFLFFMAFCVFA
                     WAQRVEIPINSQWRFAYYGSSAGAGAESVSFNDSAWGTVDLPHTWNALDGQDGGSNYA
                     RGFGWYRRHITIGREYEGKRIYLFFESVGKKADVYCNGQPAGTHLGAYAAFCFDITNL
                     VVLDADNVIAVRADNANTSSVSPLNIIPQSGDFNQWGGICRTVRLVITEPVHISPLDY
                     ASPGVYLTPTNVSRASADLSIKVLVRNTAPLGQTARIRATVLDADNIPVDTLQTDQIL
                     PQDSTQAVMLNTVISRPHLWNGRADPYLYKVIAEVEVNGIVTDGVEQPLGFRYFSVHP
                     DAGFFLNGQYLDLHGVAMHEDRQNKGRAISNADRQQDIELMLEMGCNWIRPSHYQHAE
                     TFYDLCDAKGIIVSSEIPIVNSITIGSADFDNNCKDQLRELIRQNYNHPSICFWLLYN
                     ELTTSGSENLIGQLHELAHHEDPVRLTTVAHNNTSDTAPWSYLTDTLAYNRYMGWYGG
                     TPQGFVVWADTIHLLRSDEAVGIMEYGAGANPFQHEIPPVYPGPSSPWHPEEYQTYYH
                     EVYWKAMKARPYLWCKTIWNGFDFAVDSRNEGGQPGLNDKGMVTRDRSLKKDAFYWYK
                     ANWTADPMVYITSRRFANRRTCPPYIKVYSNCQTVELFINGRSMGTKTASDCIFQWDG
                     NIVLRPGSNQIRAVGTAGSTVCEDSCVWNLDVVENYAPASVWASHYESQNPPENTLDG
                     SLGTRWAGNNYPWIKYDLGQIRQLQKIGIAFYLGNQRIYYFDIEASGDNVSWTTVLTG
                     GQSSGTASGIEEFDIADTEARYIRIQNLGNTGPSPSWASFYEVEFYGHPAIDCAFMRS
                     LHCGLAADLSGPAGRLDCAVNSVDFAEIARQWLDSYIETVVPAEPDAADLEAYWPLDG
                     NYEDITGHHHASLASGSGTPVFVNPGRRGQAVDFDGASGLACADSTGIHLLQGGTVSA
                     WIKSSNETDSWACVIAKGLTAWRLIRNDVGNTMCFHFNPASGTEFQANGITPVLDGQW
                     HHVMAVYDGSRIRLYVDGQLDAAATTGGAVLRTNADPVYIGSRADNLTGRAWNGQIDE
                     VRIYNRALTDSERLWLSEGDSFVRVPDPPRPADLVPDGRIDMQDLLQFIDWWMISTL"
BASE COUNT          878 a          958 c         1061 g          959 t
ORIGIN      
        1 acaattacgg tctgtcggcc gagacaatcg gcggccagta ctatggggtt accggtattc
       61 gtccgtgcat tgacccggcg ttcgcaggga atgcggctaa ttttgacaat accggctcct
      121 cttactgcaa ggtggatttg gcagatttag ccctgctggc ttccaaatgg cttaacaacg
      181 gcctttacac agccccgtag atgtttaggt gatgtcggca gaaaccaact aaagccggaa
      241 tcctgttttg ggattccggc ttttattagg aatgcccatt atccaagggg ggttattttt
      301 cttgaatttg actgttgacc ataaaacctc tgttgttaca cacagaaccg cgttctatac
      361 ctgtaacaga cggctcagcc atgctgctgt gtatgttatg tttctgtttt ttatggcatt
      421 ctgtgttttc gcatgggcgc agcgcgtcga aataccgatt aacagccaat ggcggtttgc
      481 ttattacggc agcagtgctg gagcgggcgc tgagtctgtc tcgtttaacg attccgcttg
      541 gggcaccgtg gacctgcctc atacatggaa tgcgctggac ggtcaggacg gcggcagcaa
      601 ttacgcacgc ggctttggat ggtaccgcag gcatatcacg attggccgag aatacgaagg
      661 caaacgcatc tatttatttt ttgaatctgt cggcaaaaaa gccgatgtgt actgcaatgg
      721 gcagccggcc ggcactcatt taggcgccta tgcggcattt tgttttgata taaccaatct
      781 ggttgttttg gatgcggaca acgtgattgc tgttcgggcg gataacgcca ataccagctc
      841 cgtcagccct ctcaatatta tcccgcaatc gggagacttc aatcagtggg ggggcatatg
      901 ccgcactgtt cggctggtta ttactgaacc ggtgcatatt tccccgctgg attatgcctc
      961 gccgggagtc tatctgacgc cgaccaacgt cagtcgggca tccgccgatt tatccatcaa
     1021 ggttctggtg cgaaatacag cgccgcttgg ccaaacggca cgaatccgcg ccactgttct
     1081 tgatgcagac aacataccgg ttgacacact gcagacggat caaatcctgc cgcaggactc
     1141 gactcaggcc gtcatgctca atacggttat cagccggccg cacttgtgga acggcagggc
     1201 ggatccctat ctttataaag tgattgcgga agtggaggtc aacggcattg ttacagatgg
     1261 tgtcgaacag ccgctggggt ttcgctattt ctctgtccat ccggatgcag gcttctttct
     1321 gaatgggcag tatctcgacc tgcatggcgt tgcgatgcat gaagacaggc agaacaaggg
     1381 ccgggcaatc agtaatgcgg atcggcagca ggatattgaa ttgatgctag aaatgggatg
     1441 caactggatt cgtcccagtc attatcagca tgcggaaaca ttttatgatc tttgtgatgc
     1501 aaagggaatt attgtttcct cggaaatacc gatcgtcaac agcataacca tcggctcagc
     1561 ggactttgat aataactgca aagatcagct gcgggaactg attcgtcaaa actacaacca
     1621 tccctcgatc tgtttctggc tgctgtacaa cgaactgacc accagcggct cagaaaacct
     1681 cattgggcag ctccatgagc tggcgcacca tgaggatccc gtgcgtttga caaccgttgc
     1741 gcacaacaat acatcggata cggccccttg gtcttatctg accgacaccc ttgcctacaa
     1801 ccgttatatg ggctggtacg gcggaacccc gcagggtttt gtcgtttggg ctgacactat
     1861 tcatctcctg cgaagcgatg aggctgtcgg cattatggaa tacggagccg gtgccaatcc
     1921 gtttcagcat gagattccgc ctgtttatcc cggaccgtct tcgccgtggc accctgaaga
     1981 gtaccagact tattatcatg aagtatattg gaaagccatg aaggcccgcc cgtatctatg
     2041 gtgcaaaacc atctggaacg gcttcgattt tgcggttgac agccgcaatg agggcggcca
     2101 gccgggcttg aatgataaag gaatggttac gcgcgaccgt tcccttaaaa aggatgcctt
     2161 ttactggtat aaagcgaact ggactgccga tccgatggtc tacatcacca gccgaagatt
     2221 tgcaaaccgg cgaacgtgtc ctccgtatat caaagtctat tccaactgtc aaacggtaga
     2281 attgtttatt aacggccggt cgatgggcac caaaaccgcc tctgactgca tcttccagtg
     2341 ggatggcaat attgtcctgc gccccggctc caatcaaatc cgggcggtgg gaacagccgg
     2401 cagcaccgtc tgtgaggaca gctgcgtatg gaacctcgat gtggttgaaa actatgcgcc
     2461 ggcatctgtt tgggccagcc attacgaatc gcaaaatcct cctgaaaaca ccctcgacgg
     2521 cagtcttgga acccgatggg ccggcaataa ttacccgtgg atcaagtatg atttgggcca
     2581 aatacggcaa ttgcagaaaa tcggcatcgc attctatctc ggaaatcaga ggatctacta
     2641 ttttgatatt gaggcgtccg gcgataatgt cagctggaca acggtgctga ccggcgggca
     2701 aagcagcggg actgcttccg ggatagagga gtttgacatc gctgatacag aggcgcggta
     2761 tatccgtatt caaaatctgg gcaataccgg tccctctccg tcatgggcca gtttttatga
     2821 ggttgaattc tacggccatc ctgcaattga ctgtgccttc atgcgttcgc tgcattgcgg
     2881 tttggcggcg gatttgagcg ggcctgccgg ccggctggat tgcgctgtta atagtgtgga
     2941 ttttgcagaa attgcccgcc agtggctgga cagttatatc gaaaccgttg tccccgctga
     3001 accggatgct gccgatcttg aagcctattg gccgctagac ggcaattacg aggatataac
     3061 gggacatcat catgcctcgt tggcatctgg aagcggaacg ccggtctttg tcaatccggg
     3121 ccgccgcggc caagcggttg attttgacgg tgccagcgga ttagcttgtg cggacagcac
     3181 gggtattcat ttacttcagg gcggtactgt ctcggcatgg attaagagca gcaatgaaac
     3241 ggattcttgg gcatgcgtta ttgcaaaagg attgaccgct tggcggttaa ttcggaacga
     3301 tgtcggcaat acaatgtgtt ttcattttaa tcctgccagc ggcaccgagt ttcaagccaa
     3361 tggcatcact cctgtgctgg atggtcaatg gcatcatgtc atggcagttt atgacggcag
     3421 ccgaataagg ctgtatgtcg atgggcaatt ggatgctgcg gccacaaccg gcggggcggt
     3481 tttgagaacc aatgccgatc cggtctatat cggcagccga gccgacaatc tcaccggcag
     3541 ggcctggaac ggccagatcg acgaggtgcg gatttataac agggcgctga ccgacagcga
     3601 acggctgtgg ctttccgaag gcgattcttt tgtacgcgtt ccagacccgc cccggccggc
     3661 ggatctggtc ccggatggca ggattgatat gcaggatctg ctgcagttta ttgattggtg
     3721 gatgataagc actttgtaga agcccaagaa aatgaccaaa ctgaaaagtt ctttgtgctg
     3781 gtgtgtttgc ttatttgtgc ttctgaatgc ggccggtctt tgggctaccg gacctcaata
     3841 tcggccggct tcggaa
//
LOCUS       DAOTBO010000177         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_19189_length_3833_cov_4.777131, whole genome shotgun sequence.
ACCESSION   DAOTBO010000177 DAOTBO010000000
VERSION     DAOTBO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19189_length_3833_cov_4.777131"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PLE88_12635"
     CDS             complement(<1..1220)
                     /locus_tag="PLE88_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77352.1"
                     /translation="MVSQHVSSIFSRQAIEICRQDPLDPAGIEKAMTFLNAALALDSS
                     SEAMAELILRVGAANCYSSADYTDSLRWALDRYLNGQADLELAAGAVRCMLERLNTRA
                     EREVLLEKLYRKYASQNSFFASDLAAQLGLLAAEKADTAAAMNYVAAAYEMNRCNQLA
                     FETMVDLSASQNLSLTPSAQLLYARAMLDLNPYDLNTAVGYADALSRFQLYHAASQAY
                     AYAVQLYEFLYPDKPLDNGLLDNWLVSCCHADQMEMRCLEIANKYRDSKRFDLMLEAA
                     AGKALTRLGQAQQAQQLLEEAGRKAESLLVIEGLALPIYPEHLAWFYSFVLEQPEKAL
                     AWSNLAYQEAPDRQGVRGVFAYTLAQSGAIEVAKQQAESLKDTDQTAALTMALVYLAE
                     DNKTQALEQLKQTVS"
     gene            complement(1351..1812)
                     /locus_tag="PLE88_12640"
     CDS             complement(1351..1812)
                     /locus_tag="PLE88_12640"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOM77353.1"
                     /translation="MNVVEFLDSKGIRYEMSQHRPTFTAQQMAAEEHVPGMNVAKPVV
                     VCADEKYYMCVLPACCKIDTDALKKALGAKKVNLADEDELTRLFGDCALGAEPPFGQL
                     YGLETLMDAALENDPYVVFQAGTHEQAIRMSLGDYVKLVQPRVLKFSYHMQ"
     gene            complement(1910..3511)
                     /gene="murJ"
                     /locus_tag="PLE88_12645"
     CDS             complement(1910..3511)
                     /gene="murJ"
                     /locus_tag="PLE88_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOM77354.1"
                     /translation="MIRGFKQTAFLTLISRILGMVRDQVFAYYFGAGWLMTAFAMGFK
                     VPNLSRRLFGEGAASASVIPIYSEELHRDPSAANRLACSVFTAVTGILTVLVVLGEIL
                     IAGYVLLFPLNEGTRVGLILTAMMLPFAIFICDTAVLGGILNVHRHFWAPAAAPVVWN
                     LIIIVFSYFAAVVLKLPSEQQVYVVAAGEVLAGIVQLAMQVIPLRTYGIILRPVWDFR
                     SKAFKQILMLMGPMMLGAAATQLNTLADDIIALSFSNDQGRPLTWGAVSYLYYAQRLY
                     QFPLGVLGISLATAIFPELSTEAARGDFRRMMQVVSRGLRMSVFVAVPAAAGLILVAR
                     PLIAVLFEHGRFTPEDTRQTAWTMSFYTVGLCGFFIQQILVRAFYSTKDSWMPALTAA
                     GSVVVNVMMNLTLIWPLGTGGLALATSICSYLQVAVLLVVLKKRHPAEMTDGLMRTMW
                     QTAAAACLMTAGGLGMLYLFKNCPLSTGFDLIRLGALVPVCTILYAGLSWFLKNEMLL
                     LLVGKRWKKETPAAESARQSQTIDE"
BASE COUNT         1053 a         1046 c          892 g          842 t
ORIGIN      
        1 gataccgtct gcttgagctg ctccagggcc tgcgtcttgt tgtcttcagc aagataaacc
       61 agcgccatcg tcagtgcggc agtctggtcg gtatccttca gcgactcggc ctgctgtttg
      121 gccacttcga ttgcaccaga ctgggccagc gtataggcaa acacgccgcg gacgccctgc
      181 cggtcgggcg cttcctggta ggccaggttg ctccaggcca gcgccttctc gggctgttcg
      241 agcacaaaac tgtaaaacca tgccagatgt tcaggataaa tcggcagtgc caatccttcg
      301 atcacaagca gtgattctgc ttttcggccg gcctcttcaa gaagctgctg agcttgttgg
      361 gcctgcccga ggcgtgtcaa tgccttgcca gctgcggctt cgagcattaa atcaaaccgt
      421 ttcgaatccc ggtatttatt ggctatttca agacaccgca tttccatctg atcagcatgg
      481 caacagctta caagccaatt gtccaaaagg ccgttatcca gcggcttgtc cggataaagg
      541 aattcgtaca attggacagc ataggcatag gcctgagaag ccgcatggta gagctgaaat
      601 cggctcagag catcagcata gccaacagcg gtgttcaaat cgtacggatt caaatccagc
      661 attgcccggg catacaggag ctgggcagag ggagtaagcg agagattctg ggaggccgat
      721 aaatcaacca ttgtttcaaa ggccagctga ttgcagcggt tcatttcata agccgcagca
      781 acgtaattca ttgccgccgc cgtatccgcc ttttcggctg ccagtaaacc caactgtgca
      841 gccaaatccg atgcgaaaaa agaattttga gaggcatact tccgatacag tttttccaac
      901 agcacctcac gctcggcccg ggtgttgagt ctctcgagca tgcatcgaac cgctccggcg
      961 gccagttcca aatcggcctg tccgttcaaa taacggtcca gtgcccagcg aagagaatct
     1021 gtataatccg cactgctgta gcaatttgcg gcaccaacac gaagtatcag ttcggccatc
     1081 gcttccgatg atgaatccag agccagagcc gcatttaaaa aggtcatcgc tttttcaatt
     1141 ccggccggat caagagggtc ctgacggcag atttcaatag cttgtcggga aaagatggac
     1201 gagacatgct gagagaccaa tgctggctgc tgggcagcgg ccggtaaaca gaatgcccat
     1261 aaaatgcata tcgttaaaca aaaactccct ttatatcgga ccataaatgc tccaaatatt
     1321 aaagcctatc attgccaatg ctattatcgg ctattgcatg tgataactga acttcaaaac
     1381 acgcggctga accagcttca cataatcgcc cagactcatc cggattgcct gttcatgggt
     1441 tcctgcctga aacaccacat aggggtcatt ttcaagggca gcatccatca gcgtttccag
     1501 cccatataac tgcccaaacg ggggttcggc tcccagcgca cagtcgccaa acaaccgagt
     1561 cagttcatct tcatcggcca gattgacttt tttggcacca agcgcttttt tgagtgcatc
     1621 ggtgtcaatc ttgcagcagg ccggaagaac gcacatataa tacttttcat cagcacacac
     1681 aacaacaggt ttggcaacat tcataccggg cacatgctct tcggcggcca tttgctgggc
     1741 agtaaacgtc ggacggtgct ggctcatttc ataacggatg cccttggaat ccagaaattc
     1801 gaccacattc atacggcacc tcctgatttt ctaaaccatg acagaacttg cacttgtctg
     1861 tcattataat accccgtgcc gcaagcgtca atccatccag ttaaaaaacc tattcgtcaa
     1921 tggtctggct ttgccttgcc gattcagctg ctggagtctc tttcttccag cgttttccga
     1981 caagcaataa aagcatctca ttttttaaaa accatgataa acctgcatag agaatcgtgc
     2041 aaaccggtac cagtgcaccc aatcgaatca aatcaaatcc tgtcgacaag ggacagtttt
     2101 tgaacaagta taacatcccc agcccccctg ctgtcatcaa gcaggcagcc gccgctgtct
     2161 gccacattgt tcgcatcaaa ccgtcggtca tctcagcagg atgccgcttt ttcagaacga
     2221 ccagaagaac agcaacctgt agataagaac agatggatgt agccaaagcc aagccgccgg
     2281 ttccaagagg ccaaatgagc gtcagattca tcatcacatt gaccacaaca gaaccggccg
     2341 ccgtcagtgc cggcatccag gaatccttgg tggagtaaaa agcccgtacc agaatctgct
     2401 ggatgaaaaa accgcacaga ccgacagtat aaaaggacat tgtccatgca gtctgacggg
     2461 tatcttcagg ggtaaatcgc ccatgctcaa acagaacagc aatcagcgga cgggctacca
     2521 gaataagccc tgctgccgca ggtacggcta caaatacaga catgcgcagc ccgcgagaaa
     2581 ccacctgcat catacggcgg aagtctccgc gagctgcctc ggtactcagt tcgggaaaaa
     2641 tggctgtagc cagcgaaatg cccagaaccc ccagcggaaa ctggtataag cgctgggcat
     2701 aatacaagta ggacactgct ccccatgtga gaggacggcc ttgatcattg gaaaaactca
     2761 aggcgataat atcgtcagcc agcgtattaa gctgcgtggc cgctgccccc agcatcatag
     2821 gccccatcag cattagaatc tgcttaaacg ccttcgagcg gaaatcccat acggggcgca
     2881 ggataatccc atacgttcgc agcggaatga cctgcattgc aagctggacg atgccggcca
     2941 gtacctcacc tgcagcgacc acgtaaacct gctgctcgga gggcagcttc aggacaaccg
     3001 cagcaaaata agaaaataca ataataatca aattccacac aaccggtgcc gccgctggtg
     3061 cccagaagtg ccgatgaaca tttaaaatac cccccagcac tgccgtatcg cagataaata
     3121 ttgcaaacgg cagcatcatc gctgtcagga ttaatcccac acgggtccct tcgttgagcg
     3181 ggaaaagcaa gacatagccg gctattagaa tttctcccag caccactaaa acggtcaaaa
     3241 ttcctgtcac cgccgtaaag acactgcagg caagccggtt ggctgctgaa gggtctcgat
     3301 ggagttcctc gctgtagatg ggtataacag aggccgatgc cgccccttca ccgaaaagac
     3361 gacgcgacag attgggaact ttaaagccca ttgcaaaggc cgtcatcagc cagccagccc
     3421 caaaataata ggcaaatacc tggtcacgca ccattcccaa aatgcggctg attaaagtca
     3481 aaaatgccgt ttgcttaaat ccgcgtatca tcgtattgaa tccctaaaaa ccggtcttta
     3541 ttcatcggca tttttgtggg attttctgct taaaaaagca agattaagtt tatagagcac
     3601 catcgtctat tctttccgtg tgtcgagaat cccaacaaat catcatgtct tttttacggc
     3661 attctgcttg tgttattctc ggcaaataga agaaaaacag agtccgcccg tatagttcca
     3721 caataataac ttaggaaagc aagctgtaaa aaattaccga gcaggagccc gagccggccg
     3781 cctgaaagga caggcaacta ctaaatattc agatgaaccg acttttgggg cag
//
LOCUS       DAOTBO010000178         3792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_19461_length_3792_cov_3.686379, whole genome shotgun sequence.
ACCESSION   DAOTBO010000178 DAOTBO010000000
VERSION     DAOTBO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19461_length_3792_cov_3.686379"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLE88_12650"
     CDS             <1..697
                     /locus_tag="PLE88_12650"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOM77355.1"
                     /translation="ETMKRTLWVCTAVLLVVSSFVQAALVDVVALQGKAVQYKNDTGV
                     WVSETRLATRTAGVWGDDPASNTLDAKKSYLQFDISGIHGTITSAVLTVYSFLSNSDG
                     KDYYIYGLKDGVNETWSADTIDWFSAPANVTNSGTAVDPSLTIQLAFCDFNADGVQDV
                     SHTDVTAFVNQDTDGLVTFIFTAGGTAYMYNVLAGTYYDAQYVPVLSIEYIVPEPATL
                     VLLGLGGLMLKRR"
     gene            968..2929
                     /locus_tag="PLE88_12655"
     CDS             968..2929
                     /locus_tag="PLE88_12655"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOM77356.1"
                     /translation="MSTSYRFWFVVAVCLAVSGLAAAGTAEIAAVQGKAADARTPNEV
                     IGETRLATRSATWPDAKKSWLQFDLSDLYFKNPGLRGHITNAKLVFYGAKSETGAKTY
                     AVSGLNDSANLENWTAAALTWNNAPANDTASPTALAAGLTTALYTGTVDVPCLDVKSE
                     TPEAARAALTDFLNTDTDGKITFILTAGGTSYLWNVGEALEPKLVIEGAIAQSYECEG
                     VGIPFSAYAAVSCRVEGATYANTNRTDSSKLSVRASSNGVKSWIKFDLRELGIDPNNL
                     KSATLRVALHAGKGGSQTCEVSAVNDNYRTNINWGETDITWNNAPGNDTSSLGLLNPA
                     QTTLMGTIAFTDGLAGQQFFIDVLPALQADTDGIVQFVLHNSPNLLDFATHDHPSGAE
                     YWPRLDILQAPYGADKPYPCPGQVVPSTLAGLSWANPDVNDINPLIRPTVYLGTEPNR
                     LKMDSVLLPVNAESVLINTANFPRFGSLVNKQTYYWIVDCRNEKTGKVIPGLVWSFYV
                     NNNEAPVVEAGNPQVLWGLPKAVALDGSVTDDGLPSSSYTVLWTQVDNGAPAAVISPA
                     DAEDTTVTITQRGDYLFKLTADDGEAQGSDTVRVVVGETPCDASHLSSGAAYAPGDVN
                     KDCIVNLEDFALLIAPNWMDCTDELTLCQ"
     gene            2994..>3792
                     /locus_tag="PLE88_12660"
     CDS             2994..>3792
                     /locus_tag="PLE88_12660"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOM77357.1"
                     /translation="MRKAYLFLTVCLLSGLLGAATVQIPADVSCRTDITNRTVNRHDS
                     NKLSIRADASAAKSWIKWTDLDGINVSTIRQAQLRLTTYETRPGLFDVSAVNDDYTTN
                     INWGERDITWDNAPANATESYTAVLAGAATYVGRMDFQNTVAGDQRFIDVTSILKADT
                     DGIVQFILHNSTGLMNMCTHDQGGTMLDGTVIGPEAARPMLFITLPPAGADWPNPELN
                     QVVSSTLSTLSWTNPEPNIPGTLITCEVFLGTEPNLLTMDRKPLTPGA"
BASE COUNT          872 a          962 c         1120 g          838 t
ORIGIN      
        1 tgaaacgatg aagcgaacgc tttgggtgtg tacagcagtg ctattggtgg tatccagttt
       61 cgtgcaggcg gctctggtag atgttgtagc cctgcaggga aaagccgtcc aatacaagaa
      121 cgatacggga gtttgggtca gcgaaacacg gcttgcaacc cgtacagcgg gggtgtgggg
      181 cgatgatccg gcttccaata cgctggatgc caagaaatcg tatctgcagt ttgacatttc
      241 gggcattcac ggtactatta ccagcgctgt cctgacggtt tattcgtttc tgagcaattc
      301 ggacggaaaa gactactaca tttacggtct gaaggacggt gttaacgaaa catggagcgc
      361 agacacgatt gattggttct cggcgcctgc caatgtaacc aacagcggga ctgctgtgga
      421 tccgagttta acgatccagc tggccttttg cgattttaat gccgacggcg tgcaggatgt
      481 gtcccatacc gatgtaaccg catttgtcaa tcaggatacc gacgggctgg taacatttat
      541 ctttacggcc ggcgggacgg cctatatgta caatgtattg gccggcacct attacgatgc
      601 ccagtatgtt ccggttttga gcatcgagta cattgtgccg gagccggcga cgctggttct
      661 gctggggctg ggcggcctga tgctcaaacg ccgctgacag tattggcaaa agggttttta
      721 ttcaaccgga gttgacacag atgccggccg ggaagaaagt attttcccgg ccggtttttt
      781 tgcggcagcg cagcagcgca tgtgccccgc agccctgttg cgcaaaatcg cttttatttt
      841 agcgcaagat tgccttgctg aaaagttcat ccctgaacga taataatagg ataggtttgg
      901 gaatgcagag attacccaat agcaggcccc tttgggcata aacgtgtttt agatgaggag
      961 aagaacaatg agcacatcgt atcgtttttg gtttgttgtg gccgtttgtc tggccgtgtc
     1021 gggtctggcg gcagccggca ctgcggagat agccgccgtg cagggcaaag ccgctgatgc
     1081 acgcactccc aatgaagtca tcggtgaaac ccgccttgca acgcgcagtg ccacttggcc
     1141 ggatgccaag aagtcatggc tgcaatttga tttgagcgat ctttatttca aaaatccggg
     1201 cctgcggggc catatcacca atgccaaact ggttttctat ggagccaaga gtgaaacagg
     1261 cgccaaaacc tatgcggtca gcgggctgaa tgattcggcc aatctggaaa actggacagc
     1321 ggcggcactg acgtggaaca atgcgccggc caacgacaca gccagcccaa cggcactggc
     1381 ggcaggactg accacggcgc tttataccgg cacggtggac gtgccctgtc tggatgtgaa
     1441 gagcgaaacg ccggaagcag cgcgggcagc cctgaccgat ttcctgaata cggataccga
     1501 tgggaagata acatttatat tgactgccgg cggcacgagc tatttatgga atgtcggcga
     1561 agcccttgaa ccgaagctgg tcatcgaagg cgctattgcc cagtcctatg aatgcgaggg
     1621 tgtgggcatt ccgttttcgg cctatgcggc agtcagctgc cgggtcgagg gtgcaaccta
     1681 tgccaatacc aaccgcacgg attccagcaa gctgagcgtg cgtgccagtt ccaacggcgt
     1741 caaatcgtgg attaagtttg atttgcggga gctggggatc gacccgaaca acctgaaatc
     1801 ggctaccctg cgggttgcgc ttcacgccgg caaaggcggc agccaaacct gtgaggtgtc
     1861 ggctgtcaac gacaattacc gcaccaacat caattggggc gaaacggaca tcacatggaa
     1921 caacgctccg ggtaatgata ccagcagctt gggccttctg aatccggcgc aaacaacgct
     1981 gatggggacc attgctttta cggatggtct ggccggccag cagttcttta ttgatgttct
     2041 gccggccctg caggcggata ccgacggcat tgtccagttt gttctgcata actcgccgaa
     2101 tctgctggat tttgccacgc acgatcatcc gtcgggtgcg gaatactggc cgcggctgga
     2161 tatcctgcag gcgccctatg gggcagataa gccctatccc tgtccgggac aggttgttcc
     2221 cagcacgtta gcgggtctgt cgtgggccaa tccggatgtc aacgatatca atccgctgat
     2281 acgccccacg gtttatttgg gaacggagcc gaaccgcctg aagatggaca gcgtgctgct
     2341 gccggtcaat gcagagtcgg tgctgattaa cacggccaat ttcccccgtt tcggctcact
     2401 ggtcaacaag caaacctatt actggattgt ggactgccgc aacgagaaaa ccggtaaagt
     2461 gattccgggt ctggtctgga gtttttatgt caataacaac gaagcgccgg ttgtcgaggc
     2521 aggaaatccg caggtgctct gggggctgcc gaaggcagtt gctctggacg gctcggtcac
     2581 cgacgacgga ctgccgagtt cttcttatac ggtgctgtgg acgcaggtcg acaacggcgc
     2641 tccggcggcg gttatcagtc cggcggatgc ggaagatacc actgttacaa taacgcagcg
     2701 cggcgattat ctgtttaagc tgacagccga tgacggcgag gcccaagggt ccgataccgt
     2761 tcgggtggtg gtcggcgaaa cgccctgtga tgcgtcgcat ctgtcaagcg gagccgctta
     2821 tgcgccgggc gatgtcaata aggactgcat tgtaaatctg gaagattttg ctctgctgat
     2881 agcgcccaac tggatggatt gtaccgatga gctgacgctg tgtcagtaag ccggatgagg
     2941 ccatttgcaa tgaacagatc agaacctatt gcgtcaaaac gaggagaata aaaatgagaa
     3001 aagcatattt gttccttaca gtgtgtctgc tcagcggcct tcttggggcg gcgacggtgc
     3061 agattccggc ggatgtgagc tgccgaaccg acattaccaa tcgtactgta aaccggcatg
     3121 acagcaataa actgagcatt cgggcagatg cctcggcggc taagtcatgg attaaatgga
     3181 ccgatctgga cggcatcaat gtcagtacaa ttcggcaagc ccagctgcgt ctgacgacgt
     3241 atgagacccg tccgggcctg ttcgatgtgt cggctgtcaa cgatgactat acgaccaata
     3301 tcaactgggg cgagcgggat attacgtggg acaatgcgcc ggccaatgca accgaaagct
     3361 atacagctgt gcttgcgggg gcggcaacct atgtcggcag aatggacttt caaaacacgg
     3421 tagccggcga tcagcgtttt attgatgtga catcgattct caaagccgac accgacggca
     3481 ttgttcagtt tatcctgcac aattcgacag ggctgatgaa tatgtgcacg cacgatcagg
     3541 gcggcactat gctggacggc actgtgatag gccctgaggc agcacggccg atgctgttta
     3601 tcacgctgcc gccggcaggg gcggactggc cgaatccgga actgaatcag gttgtcagca
     3661 gcactctgag caccctgtca tggaccaatc ccgagcccaa tattccagga acgctgatta
     3721 cctgcgaggt ttttctcggt acagaaccca atctgttgac gatggacagg aaaccgctga
     3781 cgccgggtgc ga
//
LOCUS       DAOTBO010000179         3766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_19622_length_3766_cov_5.604689, whole genome shotgun sequence.
ACCESSION   DAOTBO010000179 DAOTBO010000000
VERSION     DAOTBO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19622_length_3766_cov_5.604689"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLE88_12665"
     CDS             <1..681
                     /locus_tag="PLE88_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77358.1"
                     /translation="DEWLADKLQAQDDLFTHYPQLKWSYANFVKQSGPAAKPEHPQRK
                     NPDKPFFDDYFNAYCHGFFAWTGTVMLDRSVCDTVGLFEPGMKRGQDNDLWYRIAYQY
                     PQVGYLSQPLAVYHLDNPVSSTQINDKIDFMLSLIGRHEELSRRFNRYEAFRPCITHM
                     LQVWLRQLTAQKRFADTRILLDRFGSYLPERFVREMGFRLLFPSLTNPIADAWMKVKR
                     SILLTGKP"
     gene            699..1865
                     /locus_tag="PLE88_12670"
     CDS             699..1865
                     /locus_tag="PLE88_12670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOM77359.1"
                     /translation="MFVTTALLSPPHIGCMQRTVNICRCLAMTGQVTMLAVSHRFDER
                     SVELCKNEFPSFYQIRLGSYDEYPPLQGAFKLKWDMHWPFSHGIQADAAGQKLFAHLI
                     ETSDLVWFHTLGAAFPFSKQTLPCRSVMDLDDINHLKYTLRSRWDSNFRFRCSAQVQA
                     LKWKRHEYRAVKTYDIVAVCSEQDRRYLNAANVRVIPNGFTASAKPHWQLPNPFRIGF
                     IGTLGYGPNYDGLVWFRDNVWPDIRRRKPQMELRIVGSPPPLQYRVEADGFTQLGYIA
                     DPADEIKSWSALIVPIHYGGGTRIKILEAFSRLCPVVATSVGAQGIDVTDGKDILLAD
                     NPRRFADCCLELSENPERGRLLAQAGWELFEQKYTWDKIAHRVRQVVEEAIVQR"
     gene            1865..2860
                     /locus_tag="PLE88_12675"
     CDS             1865..2860
                     /locus_tag="PLE88_12675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77360.1"
                     /translation="MQQTIQISAVIPAYNAQKYIARAIESILRQSRPADEIIVVDDGS
                     TDDTAAVVRSYGDKVRLISQSNGGASAARNAGIRAAKGNWIAFLDADDEWLPEKLLLQ
                     TELLLKHPDLVWITGNYLQCLCDENRIAEYTPVPTCIQCLKGRDYYESYLFAVQLYEW
                     GHIDCMLIQKKTLEELGLFSTEMPIAEDIDLWLRIAYRHPKVGFTPYPLAVHHLLEFH
                     TLMTSPRPAFLYQNFIRRHFQLAQKEEVIEQFIPAAGAIMRRWIRGMLFEGRKEEIRG
                     FLVQYPQCFSVCYRCFIYLLTVFPMITVCLLKIASKIVRTLKIRRRLTRRPPKVI"
     gene            complement(2885..3739)
                     /locus_tag="PLE88_12680"
     CDS             complement(2885..3739)
                     /locus_tag="PLE88_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM77361.1"
                     /translation="MLIKYAKVYLHTDSEKSVMVLQSQLMKKKGFTLIELVTVIAAVA
                     LLVSVILPALNNAKLATKQAVCSAQMKQWALATLTYAAENDSEIVPYADTCDYTNGGN
                     ALVPETYWYNRLSCYLTEESYGSWGMDHKLRRCPMAKKNWGEKAVWIGVYYGLFASER
                     APFVFLNEWNGSILKKKSNPVNALSIKSPANYLMMLDTQRDHVFESFQWKWDTDFDGD
                     GLNDSNTGILSVNLYPFNWALPKIHRGGCNVALFDGHTEWIRYQTFWEIGEDGYPVHP
                     YWWNQNRP"
BASE COUNT          923 a          867 c          958 g         1018 t
ORIGIN      
        1 gatgagtggc tggccgacaa gctgcaggca caagacgatc tttttaccca ttacccgcag
       61 ctcaagtgga gctatgccaa ttttgtcaaa cagagcggcc cggctgccaa accggagcat
      121 ccgcaacgga aaaatccgga caaaccgttt tttgacgatt attttaatgc ctattgtcac
      181 ggcttttttg cttggacagg taccgtaatg ctcgatcgct ctgtatgcga tacagtcggc
      241 ctgtttgaac cgggtatgaa gcgcggacag gataatgacc tgtggtatcg gatagcttac
      301 caatacccgc aggtcggcta cctatcgcag ccgctggctg tttatcattt ggataatccc
      361 gtcagctcaa cacagattaa cgacaaaatt gattttatgc tcagtctgat aggccgccat
      421 gaggagcttt ccagaaggtt caatcgctat gaggcttttc ggccctgcat aacgcatatg
      481 ctgcaggtct ggcttcgcca gctgacagcc caaaagcggt ttgctgacac acggattctg
      541 ctggaccggt tcggcagcta tctgccggag cggtttgtcc gtgaaatggg ctttcggctg
      601 ttgtttccgt ctttgacaaa tccgattgcc gatgcatgga tgaaagttaa aaggtcaatt
      661 ctgctgacag gcaagccgtg accgataagc cgaaaatagt gtttgttacc acagcattgc
      721 tctcgccgcc gcatatcggc tgcatgcagc ggacggtcaa tatctgccgc tgtctggcta
      781 tgacagggca ggtaacaatg ctggcggtat cacatcggtt tgatgagcgc tcggttgaac
      841 tgtgcaaaaa cgagtttccg tctttttatc agattagact tggctcctat gatgagtacc
      901 cgcctttgca gggggcattt aagctcaaat gggatatgca ttggccgttc agtcatggca
      961 tacaggctga tgctgccggc caaaagctgt ttgctcatct gattgagacc agtgatcttg
     1021 tctggtttca cacccttggc gcggcgtttc ctttcagcaa acagacgctg ccgtgtcggt
     1081 cggtgatgga tttggatgat atcaatcatc taaaatatac tctgcggtcg cgctgggatt
     1141 ctaattttcg cttccgctgt tcggctcagg tgcaggcgct gaaatggaaa cggcacgagt
     1201 atcgggctgt caaaacatat gatattgttg ctgtttgcag tgaacaggac cgaagatatc
     1261 tgaatgctgc caatgtgcgg gtgattccca acggttttac ggcatcggca aagccgcact
     1321 ggcagctgcc caatccgttc cgaatcggct ttatcggcac actcggttac ggtcccaatt
     1381 atgacgggct ggtctggttt cgggacaacg tgtggccgga catccgccgc cgcaagcccc
     1441 aaatggagct tcgtatcgtc ggttcgcctc ctccactgca ataccgtgtg gaagcggatg
     1501 gcttcacgca gttgggctat atagccgatc cggccgacga gataaaaagc tggtctgcgc
     1561 tgattgtgcc gattcattac ggcgggggga cccgcatcaa gatactcgag gcattcagcc
     1621 gtctgtgtcc ggttgttgca acatcggtag gggcacaggg catcgatgta accgacggca
     1681 aagatatcct gttggctgat aatccgcgcc gatttgccga ctgctgcctg gaactgtcag
     1741 aaaatcccga aagaggcagg ctgctggctc aggctggctg ggagctgttt gagcagaaat
     1801 acacgtggga taaaattgcg cacagggtgc gtcaggttgt ggaggaagca attgttcagc
     1861 gatgatgcag cagaccattc agatcagtgc tgttattccg gcctataatg cccaaaaata
     1921 tattgcacgg gcaatagaaa gcattctgcg gcaaagccgg ccggcagatg aaattattgt
     1981 tgttgatgac ggctcgaccg acgatactgc tgcagtggtc cgctcatatg gagacaaggt
     2041 ccggctgatc agccaatcca acggcggcgc cagtgctgca cgcaatgccg gcatacgggc
     2101 cgcaaaggga aactggattg cctttctcga tgccgatgac gaatggttgc cggaaaagtt
     2161 gcttttacag acggagttgt tactgaaaca tcctgatttg gtctggataa cgggtaacta
     2221 tttgcaatgc ttgtgcgatg aaaatcgaat tgctgaatac acgcccgttc caacttgtat
     2281 tcaatgtctt aaggggagag attattacga gagttattta tttgcagttc aattgtatga
     2341 atggggtcat attgattgta tgttgataca aaagaagacc cttgaggaac taggcctatt
     2401 ttctacagaa atgccgatag cagaggatat cgatttatgg cttcggatag cgtatagaca
     2461 tccgaaagtt ggttttacac cgtatcctct tgcggttcat catttgcttg aattccatac
     2521 actgatgaca tcgccgcgtc ctgccttttt gtatcagaat tttatccgca gacatttcca
     2581 attggctcaa aaggaagagg taattgaaca atttatccct gctgctgggg cgattatgcg
     2641 tcgctggatt cgagggatgc tgtttgaagg ccgtaaagaa gaaatacgag gttttttagt
     2701 tcagtatcct caatgctttt ccgtatgtta tcgctgtttc atttatttgt tgactgtttt
     2761 tccaatgata actgtttgtt tgctaaaaat agcatctaag atcgtacgca ctttaaaaat
     2821 acggagacga cttactcgtc ggccgccgaa ggtcatataa ggcgattttt taagactgat
     2881 gcgtttatgg ccggttttga ttccaccaat agggatgtac aggatagcca tcctctccga
     2941 tttcccaaaa ggtctggtag cgaatccatt ccgtatggcc atcaaataag gcgacattgc
     3001 atcccccgcg gtggatcttg ggtaaagccc aattaaaggg atataaattt acagaaagaa
     3061 taccggtatt ggagtcgttt aatccatccc cgtcaaaatc agtgtcccat ttccattgaa
     3121 acgactcaaa aacgtggtca cgctgcgtgt caagcatcat gagataattg gcgggggact
     3181 taatggaaag ggcatttact ggattactct tttttttcaa aatagatcca ttccactcgt
     3241 taagaaaaac gaaaggtgct ctttcagatg caaagagacc ataatacaca ccaatccaca
     3301 cggccttttc tccccagttt ttcttggcca tcggacatcg acggagcttg tggtccattc
     3361 cccagcttcc ataggattct tctgtcagat agcaggaaag acggttgtac caataggttt
     3421 caggaaccag agcatttccg ccatttgtgt aatcacatgt gtcagcgtat ggaacgattt
     3481 cgctgtcgtt ttccgctgca taagttagtg ttgccagcgc ccattgcttc atctgggcgg
     3541 agcagacggc ttgtttggtc gccaatttgg cattgttcag ggccggaaga ataaccgata
     3601 caagcaaggc aaccgccgca attacggtca ctaattcgat gagcgtaaat ccctttttct
     3661 tcattagttg agactgtagc accataacgg atttttctga atctgtgtgc aagtaaactt
     3721 tcgcatattt tattaacatt ttaagcggct ggagctaatg cttcca
//
LOCUS       DAOTBO010000180         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_19845_length_3730_cov_5.003265, whole genome shotgun sequence.
ACCESSION   DAOTBO010000180 DAOTBO010000000
VERSION     DAOTBO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19845_length_3730_cov_5.003265"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..3657
                     /locus_tag="PLE88_12685"
     CDS             361..3657
                     /locus_tag="PLE88_12685"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOM77362.1"
                     /translation="MAILGFLFCQSADASSTAHNKAVMPQEFSNPPQEYGVRCWWWWL
                     NSNVTKEAITRDLEAMKAKGFSGALIFDAGGAEQRGNRQVPAGPRYASEAWRQLLTHA
                     VREAERLGLELGLSIQSGWNLGGPSITPQLAAKRLTFSEIEVSGPAVFADKLPQPPTI
                     DNFYRDIAVLAWPVKPASAAQLTASFQQTDYPAANAADGNPATYWVSGTDQPQLGPAV
                     NRPQHLLISLEKSARIRGLQITPHKGYGPRKCQLQVSRDGQSFESICSFEMKDDIYQE
                     TFESAAVRHIRLVITGAFDPRYPETPRNVQIAEIALLDEQGRNLLRPSARKPIQNLEL
                     KAMYEELGMSAPDCRFLLDDEPPQEGEEDVHTEEILNLTDKLDAEGRLHWQIPQGRWT
                     ILRCGFTPTEARVSTSSQGWEGRVLDYMNADTFRVYWNEVVEPLLADIGPSVGRTLKY
                     LQTDSWECGGMNWTDSFAAEFQKRRGYDPIVYLPVIAGKIVDSRIASNAFLADFRKTI
                     GDCVADNHYQVFADLAHQHSLGIQPESGGPHAGPFDAIKCLGRSDMMMSEFWVPSPHR
                     PRPVDRFYVKQAASAAHIYGRPLVGAESFTSIGPHWNDVLWKSQKPSMDHEFCSGLNL
                     IFLHTFTCSPPAMGLPGQEYFAGTHINPQVTWWDYSDAFFEYIARCQYLLQKGLFEAD
                     VLYYYGDHIPNVARLKEDDPAKVLPGFDYDLTNEEILLQLNVEDGRITVPGGLRYRLL
                     VLPDHKVLSLAALEKIDQLVRQGATVLGPKPQRLVSLTGGQAAQEKFKLLAEQLWPVH
                     PSEKGQQQVGSGRVIWGKTARQVLIDDGILPDFEYQAAADAALDYIHYTIDGMDIYFV
                     CNQTEQMQTAVCRFRITDRQAELWNPLTGQIRRNILQRQTGSQTEIPLRFEPYGSLFV
                     LFQDKTMPEAGVQTANYFDCQSVLTIAGPWRVHFDPIWGGPSETVFERLISWTEHPDE
                     NIRFYSGKAIYETDFVFEHQPDNQATTYWLDLGRVEDIGIARIRLNGQDLGIVWTPPF
                     RAEISTIIKAGKNRLEVDVINSWRNRLIGDRDLPADQRRTKTNIRVTPQWTLLESGLL
                     GPVQILAAKK"
BASE COUNT          899 a          958 c          987 g          886 t
ORIGIN      
        1 gaagcattag ctccagccgc ttaaaatgtt aataaaatat gcgaaagttt actaaattgc
       61 aattcatctt ccaaaatgat gcggccccga agattttctg cggaaaatga gcagcatgaa
      121 atataccgtt tctgcctgag aggtcttttc ggcaacaaac cggtttattg ctatcggcat
      181 ttgtttgccc tataatactt tgctatggga tgatatgacc tattggcata aagtttcaat
      241 gcgatactta accggcatct gatgggtaat tctgcgggct gctataaaac gtttagctga
      301 gtttttacaa acgaaaggta caccatgcac aaatcggctg caactttgtc tggatttgct
      361 ttggcaatcc ttggattttt attctgccaa tcagcagatg catcctctac ggcacacaat
      421 aaagctgtaa tgccgcaaga gttttcaaat cccccgcagg aatatggcgt gcgatgctgg
      481 tggtggtggc tcaacagcaa tgttaccaaa gaagccatca cccgcgacct tgaggcaatg
      541 aaagccaagg gattcagcgg agcgctgatt tttgatgccg gcggggcaga gcagcgcggc
      601 aatcggcagg ttccggctgg tccccgttac gcgtctgagg cctggcggca actgctcacc
      661 catgccgtcc gagaagcaga gcggctgggt ttggagctgg gtttgagtat ccagagcggc
      721 tggaatctgg gcgggccgtc aatcacacct cagctggcgg caaagcggct gacattttca
      781 gaaatagaag tttcgggccc agcagtattt gctgataaat tgccccagcc ccccacaatc
      841 gacaatttct atcgggatat cgccgtactg gcatggcctg tcaagccggc atcagcggct
      901 caattaaccg ccagttttca gcagaccgat taccctgccg ctaacgccgc tgacggcaat
      961 ccggcgacat attgggtcag cggcactgac cagccccagc tggggcctgc cgtcaatcgg
     1021 ccgcagcatc tgctaatatc tctggaaaaa tccgctcgga ttagaggcct tcagattaca
     1081 ccccacaagg gatatggtcc tcgtaaatgc caattgcagg tctccagaga cggtcaaagt
     1141 tttgaatcta tttgttcatt tgagatgaaa gatgatatct atcaggaaac gtttgaatcc
     1201 gcagcagtcc gccatattcg tctggtcatt accggtgcct ttgacccgcg ttaccccgag
     1261 actccccgca atgtccagat tgcagaaatc gctttgctgg atgaacaggg acgcaatctg
     1321 ctgcggccgt cggctcgcaa gccgattcag aatttagaac tcaaagcaat gtatgaagaa
     1381 ttgggcatgt cagctccgga ttgccgattt ttgctggatg atgagccgcc gcaggaaggc
     1441 gaagaagatg tccacacgga agagattctt aatctgacag ataagctgga tgccgaaggc
     1501 cggctgcatt ggcagattcc ccaaggccgc tggacaattc tgcgatgcgg ctttacgcct
     1561 acagaggctc gtgtcagtac ttccagccaa ggctgggaag gacgcgtgct ggattatatg
     1621 aatgccgata cctttcgggt gtactggaat gaagtagtgg agccgctgct ggcagatatc
     1681 ggcccttcgg ttggacgcac gcttaaatat cttcagacag acagctggga gtgcggagga
     1741 atgaactgga cagacagctt tgctgctgag tttcagaagc gccgcgggta tgatccgatt
     1801 gtctatcttc cggtgattgc cggaaaaatt gttgacagcc ggattgccag caatgccttt
     1861 ctggctgact tccgcaagac catcggcgac tgtgttgcgg acaatcatta tcaggtcttt
     1921 gccgatctgg cgcatcagca cagtctgggt attcagcccg aatcaggcgg cccccacgcc
     1981 ggaccgtttg atgctattaa atgcctcgga cgcagcgata tgatgatgag cgagttctgg
     2041 gtaccctcac cgcatcggcc tcggccggtg gaccgctttt atgttaagca ggcggcttcg
     2101 gcagcccata tttacggccg gccgcttgtc ggtgcggagt cgtttacatc catcggaccg
     2161 cattggaatg atgtgttgtg gaaatcccag aagcccagca tggatcatga attctgctcc
     2221 ggactgaacc tgatttttct gcacactttt acctgctcgc cgcccgcgat ggggcttccc
     2281 ggacaggaat atttcgctgg tacgcatatc aatccccaag taacttggtg ggactattcg
     2341 gatgcttttt ttgagtatat cgcgcgctgc cagtatcttc tgcagaaggg actatttgaa
     2401 gcggatgtat tgtattacta cggtgaccat atacccaatg tagcccgcct caaggaagac
     2461 gatcctgcta aagtgctgcc gggatttgac tatgacctga ccaatgagga gatcctgctg
     2521 cagctgaatg tcgaagacgg acgtattaca gttcccggcg gcctgcgcta tcggctattg
     2581 gtgctgccgg accacaaggt gctgtccctg gcggccctgg aaaaaatcga ccagcttgtc
     2641 cgtcaaggcg ccaccgtact tggcccgaaa ccccagcggc tggtttccct gaccggcgga
     2701 caggcggcgc aggaaaaatt caaattactg gccgaacagc tttggcctgt tcatccttcg
     2761 gaaaaggggc agcagcaagt cggcagcgga cgggtcatct ggggtaaaac ggcccgccaa
     2821 gtccttatcg atgacggcat cctgcctgat ttcgagtacc aagccgctgc agatgcggcg
     2881 cttgattata tccattatac aatagatgga atggatatct acttcgtctg caaccagact
     2941 gaacagatgc agactgccgt ctgcagattc cgtataactg accggcaggc tgagctttgg
     3001 aatccgctga cagggcaaat ccgccggaat atactccaaa ggcagaccgg cagtcagaca
     3061 gaaatccccc tgcgatttga gccctatgga tcgctattcg tcctcttcca agacaaaact
     3121 atgccggaag ctggggtgca gacagcaaat tatttcgact gccaatctgt tttgacgata
     3181 gccggtccgt ggcgtgtaca tttcgatccg atctggggcg gtccgtcaga gacagtcttt
     3241 gaacgtctga taagctggac agagcatccg gatgaaaaca ttcgattcta ttccggcaag
     3301 gccatttacg aaaccgattt tgtattcgaa catcagccgg acaatcaggc cacaacatac
     3361 tggcttgatt taggacgtgt agaggatatc ggcatcgcca ggattcgtct caatgggcag
     3421 gatttaggca ttgtctggac gccgccgttt cgagcagaaa tctcaactat tatcaaagcc
     3481 ggcaaaaacc gtctggaagt cgatgttatt aactcgtggc gcaaccggct gataggcgat
     3541 cgtgatttgc ctgctgacca gcggcgtacc aaaacaaata tccgcgttac gcctcaatgg
     3601 acccttttgg aatccggcct attaggccct gtgcagattc tggctgcaaa aaaataaaat
     3661 cccaaacgcc gagaccccag aagtttttac agttgagccg tccatcggca actgctacca
     3721 gcctggagtg
//
LOCUS       DAOTBO010000181         3715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_19954_length_3715_cov_4.899454, whole genome shotgun sequence.
ACCESSION   DAOTBO010000181 DAOTBO010000000
VERSION     DAOTBO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19954_length_3715_cov_4.899454"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLE88_12690"
     CDS             complement(<1..785)
                     /locus_tag="PLE88_12690"
                     /inference="COORDINATES: protein motif:HMM:NF025965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HOM77363.1"
                     /translation="MQTKTIRPPFLLPAGLLVCGLLCAASPFNPPERIRAERPAAETR
                     MARPLRYRPDNLDFVIENGSEFFNRPLYGGPTAFRADAGDKPEFSLYLPGQGGNLRLG
                     IQSSGRGKWLHEADRIVSRYRPQAMLYEIHDALLGDGVIQLSAMALYEGDGLIVKAEL
                     QNAGNKPLELVFAFGGAGGKRGQRGGDIGCESEPISQFFQLRPENCRGNQFTVNGNQF
                     LLQSSIASIAGIFPKGAKLAIADAQHWNAFLQLLASAGTFCQLP"
     gene            complement(775..3276)
                     /locus_tag="PLE88_12695"
     CDS             complement(775..3276)
                     /locus_tag="PLE88_12695"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOM77364.1"
                     /translation="MKKRQFGLFLLGLMLAWPCLAEPMTAEQGWQMVPEILQRIVPPT
                     FPDKDFPITQFGAVGNGDRDCTEAFRKAIDACSSAGGGRVVVPKGIYLTGAIHLKSNV
                     NLYLEKEAVISFFTNPDDYLPAVFARFEGTECWNYSPLVYAFEQDNIAVTGSGTLDGN
                     AGITNWWRWKWTQKADVEKLTQQAEQQMPVEQRVFGSGHKLRPNMIVPYRCKNVLIEG
                     VTIKNSPMWHIHPVLCQNVTVRNVTVIGHGPNNDGCNPESCRDVLIENCFFDTGDDCI
                     AIKSGRNADGRRVNVPSENIIVRKCTMKDGHGGVVIGSEVSGSARNIFAEDCVMDSPN
                     LDRGLRLKTNSVRGGVIENIFMRNVTMPQVKEAALLIDFYYQEGDSGRFDPVVRNIFM
                     TNVRCAKSRYPWLMRGYPRAPIRNVVLENCVFETMEQPGIAEAVEGFIVRNDAPSAGR
                     TSGQWSEKMIASVIRQNPQAWMIDFSPSPRWKYAIGVVLKGIWEAGIRSGREDYLQYV
                     QGYYDTLITEDGRIQTYELETYNIDNINPGKTLIDLYQRWPQDKYKRAIETLRDQMRT
                     HPRVAEGGFWHKKIYPHQMWLDGIYMGSPFLAQYADVFEEPALFDDVARQIILMNRVS
                     RDQRTGLLYHGWDESRSQKWADPQTGRSPNFWGRAMGWYAMALVDTLDFFPKDHPQRG
                     EIITILNQLVEAVCRVQDEQTGLWYQVLDQSGRQGNYLESSCSSMFVYAMFKAVRHGY
                     IPQRYFQNAQRGYQGILKQFVETTADGLVHLHKGCAVAGLGGNPYRDGSFEYYISEAV
                     RTDDPKAVGPFILASLEYEAAGSAIETTAVQRYAN"
     gene            complement(3379..>3715)
                     /locus_tag="PLE88_12700"
     CDS             complement(3379..>3715)
                     /locus_tag="PLE88_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOM77365.1"
                     /translation="NCTITGQPGVKTYLGRPWRDYANVIFLNTEMSDTVRPEGWHDWD
                     KPYRQTTARYGEYNSRGAGAAPEARVPWARRLTAEQAADITVQAVLGGTDGWNPLAKP
                     AQAPAPPAQ"
BASE COUNT          809 a         1131 c          946 g          829 t
ORIGIN      
        1 ggcagctgac agaaagtccc cgcagaagcc agcagctgca aaaaggcatt ccagtgttgg
       61 gcatcggcga tcgccagctt ggcgcctttg gggaaaatgc ctgctatgga agcgatgctg
      121 ctttggagaa gaaattgatt gccatttacc gtaaactgat ttccccggca attttccggc
      181 cgaagctgga aaaactgact gatcggttcg ctttcacagc cgatatctcc cccgcgctgg
      241 ccgcgtttgc cgccggctcc gccgaaagcg aacaccaatt ccaagggctt attgccggca
      301 ttctgaagct cagcctttac aatcagacca tctccctcat acaaagccat cgctgacaat
      361 tggatgacgc cgtcgcccag aagggcatca tgaatctcat agagcatcgc ctgcggccga
      421 taccggctga caatacggtc tgcctcgtga agccatttgc cccggccgga agactgaatg
      481 cccagccgca gattgccccc ctgccccggc agatacagcg aaaactccgg cttatccccc
      541 gcatcggcac ggaatgcagt aggcccgccg tacagcggcc ggttgaaaaa ctcgctgccg
      601 ttttcaatca caaaatcgag attgtccggc cgataccgca gcggccgagc catccgcgtc
      661 tctgctgccg gacgttccgc acgaatacgc tccggcggat tgaacggaga ggccgcacag
      721 agcagcccgc agacgagcag ccccgccggc aaaagaaagg gtggcctgat tgttttagtt
      781 tgcatagcgc tgcactgccg tggtctcaat cgccgaccct gccgcttcgt actccagact
      841 ggctaaaata aacggcccga ccgccttggg atcatcggtg cggactgctt cgctgatata
      901 gtattcaaag gagccatctc ggtatggatt gccgcccaag ccggccactg cgcatccctt
      961 gtgcagatga accagaccat cggctgttgt ctccacaaac tgctttaata tgccctgata
     1021 tccccgttgg gcattttgga aataccgctg cgggatgtac ccgtgacgga ccgccttgaa
     1081 catcgcataa acaaacatcg aagaacaaga ggattccaaa taattcccct gccggccgga
     1141 ttgatccagc acctgatacc acaatcctgt ctgctcatcc tgcacacggc agaccgcctc
     1201 gaccagctga ttgagaatcg tgataatctc accgcgctgg gggtggtctt tggggaaaaa
     1261 atccagcgta tccaccagcg ccatcgcata ccagcccata gcccggcccc agaagttcgg
     1321 cgagcgtccg gtctggggat ctgcccattt ttggctgcgc gattcatccc agccgtgata
     1381 cagcaggccg gttcgctggt cgcgggaaac ccggttcatc aaaataatct gccgcgctac
     1441 atcatcaaac agcgccggct cctcaaatac atcggcatac tgcgccaaaa aagggctgcc
     1501 catatagatg ccgtccagcc acatctggtg cggatatatc ttcttgtgcc agaatccccc
     1561 ttccgccaca cggggatgcg tccgcatctg gtcccgcagc gtttcgatgg cccgcttata
     1621 cttgtcctgc ggccagcgct gatacaaatc gatcagcgtc ttgccgggat tgatattatc
     1681 gatattgtag gtctccagct cgtaggtctg aatgcggccg tcttctgtta tcagcgtatc
     1741 atagtagccc tgcacatact gcaaatagtc ctcccggccg ctgcgtatcc ccgcctccca
     1801 gatgcccttg agaaccactc caatcgcata tttccatcgg ggcgaagggc tgaaatcaat
     1861 catccatgcc tgcggattct gccgaatgac cgatgcgatc atcttctcgg accactgccc
     1921 ggatgtccga ccggctgacg gggcatcgtt tcggacaatg aaaccttcta ctgcctcggc
     1981 aatgccgggc tgctccattg tttcaaaaac acagttttca agcacgacat tgcgaatcgg
     2041 cgctctggga taaccccgca tcagccatgg atatcggctt ttggcacagc ggacattggt
     2101 cataaagatg ttgcggacca ccggatcaaa ccggccgctg tcgccttcct gataataaaa
     2161 gtctatcagc agcgccgctt ccttaacctg cggcatcgtt acattgcgca taaagatatt
     2221 ttcaatcaca ccgccccgca ccgaattggt cttcagccgc aggccgcgat ccagattggg
     2281 gctgtccatc acacagtctt cggcaaaaat gttgcgggcg ctgccggata cctcgctgcc
     2341 gataaccact ccgccgtggc cgtccttcat ggtgcatttg cggacaatga tattctccga
     2401 aggaacgttc acgcgccgcc cgtcggcgtt gcggcccgat ttgatggcga tgcagtcgtc
     2461 gccggtatca aaaaagcagt tttcaatcag cacatcccgg cacgattccg gattgcaccc
     2521 gtcgttgttg ggcccatggc ctataaccgt gacattgcgg acggtaacat tttgacagag
     2581 caccggatga atatgccaca tcgggctgtt ctttatcgtt accccttcaa tcagaacatt
     2641 tttgcagcgg tagggcacaa tcatattggg ccgcagctta tgaccgctgc cgaaaacgcg
     2701 ctgttcaacc ggcatctgct gttcggcctg ctgggtcagc ttctccacat cggccttctg
     2761 cgtccatttc caccgccacc aattggtgat accggcgttg ccgtccagcg tgccgctgcc
     2821 ggtaacggca atattgtcct gctcgaaggc atacaccagc ggcgaataat tccagcattc
     2881 ggttccttca aatcgcgcaa ataccgccgg cagataatcg tctggattgg taaagaagct
     2941 gatgacggct tctttttcca gatacagatt tacattgctt tttaaatgaa tcgcaccggt
     3001 cagatagatg cctttgggca caaccacacg ccccccgccg gccgaactgc aggcgtcgat
     3061 ggcctttcga aaggcctccg tgcagtctct gtcgccgttg ccgacggctc cgaactgggt
     3121 aatgggaaaa tctttatccg gaaaggtcgg cggcacaatc cgctgcagga tttccggcac
     3181 catctgccag ccctgttccg ctgtcatcgg ttcggcaagg cagggccatg ccagcatcaa
     3241 gccaagaaga aataatccga attggcgttt tttcatatct tcacctcttt ttagttattg
     3301 gagatatttt agtatctgtc gtcagagagt aaactgaata attttgagag acgccgccgt
     3361 tgagtgcctt attggccgtc attgtgccgg cggtgcaggg gcttgagccg gctttgccag
     3421 aggattccag ccgtcagtcc cgccaagaac cgcctgcaca gtaatatccg ccgcctgttc
     3481 cgctgtcagc cggcgtgccc aaggcacgcg tgcttcaggt gctgctccgg caccgcggct
     3541 gttatactcg ccatagcggg ccgtcgtctg gcgatagggc ttatcccaat cgtgccagcc
     3601 ctccggccga accgtgtcgg acatctcggt attgagaaat atcacattgg cataatcccg
     3661 ccacggcctt cccagatagg tcttgacgcc cggctgaccg gtaatggtac aattg
//
LOCUS       DAOTBO010000182         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20074_length_3698_cov_3.669503, whole genome shotgun sequence.
ACCESSION   DAOTBO010000182 DAOTBO010000000
VERSION     DAOTBO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20074_length_3698_cov_3.669503"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLE88_12705"
     CDS             complement(<1..193)
                     /locus_tag="PLE88_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM77366.1"
                     /translation="MTQKRRRFTAEFKARAVRAALREDKTLAQLATEFDVHPNQITEW
                     KRQALEKAAGEELAEKAKKE"
     gene            complement(281..1711)
                     /locus_tag="PLE88_12710"
     CDS             complement(281..1711)
                     /locus_tag="PLE88_12710"
                     /inference="COORDINATES: protein motif:HMM:NF018528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HOM77367.1"
                     /translation="MFTRRDFLKTTAAAAAGFSMPRSGANAQSLPAFASKRPPQEKRT
                     FVSPIVEQTIREVTSFMKDPELAWMFENCFPNTLDTTTDFEIIDGKPDTFIITGDIDA
                     MWLRDSTCQVWPYMPLIQKEEKLRTLIQGLINRQVKCVLLDPYANAFYKDLSRISEWN
                     QDRPKLQPGVHERKWEIDSLCYVVRLGYEYHRLTGDVSCFDKDWDRAMRLIVATFKTE
                     QRKDGQSPYRFIRTTSRMTDAPVYDGTGYPVRPVGMICSMFRPSDDATILPFLVPSNL
                     FAVQALRQLAEIYTKAVQDAAFARACTSLADEVQEAVMKWATADHLDYGRIYAYEADG
                     FGNRLFMDDANVPSLMSLSYLGIHKPQDEIYQRTRTFLLSENNPWYLRGKAAEGYGSP
                     HTGKNSIWPMGIILRALTSESMDEIHLCLRMLKATHAGTGFMHESFNKDNPADFSRSW
                     FAWANTLFGELILEVYQKYPSLLKEN"
     gene            complement(1733..2839)
                     /locus_tag="PLE88_12715"
     CDS             complement(1733..2839)
                     /locus_tag="PLE88_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOM77368.1"
                     /translation="MQDHRIVLTLDAGGTNFVFSAIQDMKEAAAPVRLPAYADNLEKS
                     LASIREGFSQVISQLPQKPAAISFAFPGPADYPNGIIDNMGNLPAYRGGVALGPYLEE
                     TFQMPVFINNDGDLFAYGEAIAGLLPKVNRMLEQAGSPKRFSKLFGITLGTGFGGGIV
                     ADGRLFIGDNSAAGEIWITRNKRHPKCFAEEGVSIRAVRGSYARVAGIDVSDAPTPKD
                     IYEIGIGRQPGNKDAARQAFEEMGEVIGDALANAITLIDGLIVIGGGLAAAYPLFIDA
                     VLKEMNGTIESYSGQKLPRIVQRCFDLENPEACRLFLEGRIKDIHVPGSKRIIKYDSL
                     KRIGIGRAVLDTSHAVSIGAYAYALNQLDNPKKK"
     gene            complement(2874..>3698)
                     /locus_tag="PLE88_12720"
     CDS             complement(2874..>3698)
                     /locus_tag="PLE88_12720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOM77369.1"
                     /translation="PIMRDVSPQGAYGRNLSFAQGFKGIGSTVSSYLVTAIAGFALFE
                     SMGWRGVFPIFFILMAVTFVWVATLKIEETKADVPPSILTSLQLLQKPVFAMAVLGIF
                     LYVGAEVCMGRFLLPLLEAMGLEKSTASKFGPAFFFMMLTVGRLAGGAVLTLLTPRTF
                     FRLSAALGLLGAGLLMLGNPSFAILGVLAAGLGFANIWPMLFSITVEEEPQRSSELSG
                     LMCMAISGGALVPLAMSKLVDSGLAAKAFIIPVICFFYLLALSLKGPSKVKPAAAQ"
BASE COUNT          888 a         1025 c          911 g          874 t
ORIGIN      
        1 cttccttctt ggccttttcc gccagttcct caccggccgc cttttccagc gcctgccgtt
       61 tccattccgt aatctggttg gggtggacat cgaactccgt ggccaactgc gccagcgtct
      121 tgtcctcccg caacgccgct cgcaccgcac gggccttgaa ttccgccgta aagcgtcgtc
      181 gtttctgtgt catcgttttc agtccctttc tgagcttttc gctccagtat acccgaaaac
      241 aattccacct taaatacctg tccagttttt ggggacacgc tcagttttct ttgagcagag
      301 aggggtattt ctgataaacc tctaaaatca gctcaccaaa cagggtattt gcccaagcaa
      361 accagcttcg cgagaaatcc gccggattgt ctttattgaa ggattcatgc ataaatcccg
      421 ttcctgcatg cgtggctttc agcatccgca ggcacaagtg aatctcatcc atgctttcac
      481 tggtcagcgc acgaaggata attcccatcg gccagatgct gtttttgccc gtatgcggac
      541 tgccgtatcc ttctgcggcc tttccccgca gataccacgg attgttttcg ctcagaagaa
      601 aggtgcgggt ccgctgatag atttcatcct gcggcttatg aattcccaaa tagctcagcg
      661 acattaagct gggcacattg gcatcatcca taaacagccg attgccgaat ccgtctgcct
      721 cataggcata aatccgtccg taatccagat ggtcagctgt tgcccatttc attacggctt
      781 cctgaacctc atcggccaga ctggtgcacg cacgggcgaa ggccgcatct tggactgctt
      841 tggtgtaaat ctcagccagc tggcgcaaag cctgaaccgc aaagagattc gagggaacca
      901 gaaacggcag gatggttgca tcatccgatg gacggaacat agagcaaatc attccgaccg
      961 gccgcaccgg ataccccgtg ccgtcataaa cgggcgcatc ggtcatccgc gaggttgtgc
     1021 gaatgaagcg gtaggggctt tggccgtctt ttcgctgttc ggtcttaaag gttgccacaa
     1081 tcagacgcat ggcgcgatcc cagtctttat caaagcagga aacatcacct gtcaggcggt
     1141 gatattcata ccccagccga accacgtagc acagagaatc gatttcccac ttccgttcat
     1201 gaacaccggg ctgcagtttg ggcctatcct gattccattc ggatatacgc gaaagatctt
     1261 tgtaaaatgc gttggcgtaa gggtccagca gaacgcactt gacctgacgg ttaataagtc
     1321 cctgaatcag tgtgcgcagc ttttcctctt tttgaatcag cggcatatag ggccagacct
     1381 gacaggttga atcgcgcagc cacattgcat caatatcgcc ggtaatgata aaagtatcgg
     1441 gtttgccgtc gataatttca aaatcagtcg ttgtatcgag tgtgttgggg aaacaattct
     1501 caaacatcca cgccaattcc ggatccttca taaaggatgt aacctctcga atcgtttgtt
     1561 ccacgatagg gctgacaaag gtccgtttct cttggggcgg acgctttgaa gcaaatgccg
     1621 gcagcgattg ggcatttgct cccgagcgcg gcattgaaaa tccggctgcc gcagcggcgg
     1681 tggttttaag gaaatctctg cgtgtaaaca ttctgcacct catacaataa agttatttct
     1741 tcttcggatt gtccaattga ttaagagcat aggcatatgc cccaatagaa accgcatggc
     1801 tggtatccaa aactgcccgt ccaatcccga ttcgcttgag tgaatcgtac ttgataattc
     1861 ttttgctgcc gggcacatgg atatctttga tgcggccttc caaaaatagc cggcaggctt
     1921 ccgggttttc caagtcaaag catcgttgga caatgcgggg cagtttttgg ccgctgtagc
     1981 tttcaatcgt cccgttcatt tctttgagaa cagcatcaat aaaaagcgga tacgctgctg
     2041 ccagcccgcc gccgataacg atgaggccat caatcagcgt gatagcattg gccagagcat
     2101 cgccgatgac ttcgcccatt tcctcaaatg cctgacgggc tgcatcttta ttgcccggct
     2161 ggcggccgat gccgatttcg tagatatcct ttggcgttgg tgcatcggaa acatcgatgc
     2221 cggcaacccg ggcgtacgaa ccgcggacag cgcggatgct gacgccttct tctgcaaagc
     2281 atttgggatg acgcttgttg cgggtaatcc agatttcgcc ggctgcggag ttgtccccaa
     2341 taaacagtct tccgtcggcg acaatcccgc ccccgaagcc ggtccccagc gtaatgccga
     2401 aaagcttgct gaaccgtttt ggggaacccg cctgttccag catgcggttg actttgggca
     2461 gcaggccggc tatcgcttcc ccgtaagcga acaaatcgcc gtcgttattg ataaaaaccg
     2521 gcatctgaaa tgtttcttcc agatagggcc ctaaggcaac gccgccgcga taggccggaa
     2581 gattgcccat attgtcgata atgccgttcg ggtagtctgc cggccctggg aaggcaaaac
     2641 tgatagctgc cggcttctgc ggcaattggg atatcacttg cgaaaaaccc tcccggatag
     2701 aagccaagct tttttccaaa ttatcggcat aagccggcag acggaccggt gctgctgctt
     2761 ccttcatatc ctgaatagcc gaaaaaacaa agttggttcc gccggcatct agcgtcagaa
     2821 caatacgatg atcttgcatt ctcttgcttc cttaaaatag ggttaaaaga gtatcactgc
     2881 gctgctgctg gtttaacctt gctggggcct ttcagcgaca gtgccagcag atagaaaaag
     2941 cagataaccg gtatgatgaa tgcttttgcg gccagcccgc tgtcgaccag cttgctcatc
     3001 gccagcggaa ccagtgcccc gccggaaata gccatgcaca tcagcccgct caattcgctg
     3061 ctgcgctgag gttcttcttc aaccgtaatc gaaaacagca tcggccagat atttgcaaat
     3121 cccaaaccag ctgccagtac gccgagaatg gcaaaggatg gattgcccag catcagcaga
     3181 ccggccccca gcaatcccag agctgctgac aagcggaaaa atgttcttgg cgtcagcaaa
     3241 gtcagtacgg caccgccggc taaacggccg accgtcagca tcataaaaaa gaacgccggt
     3301 ccgaattttg aggcggtgga tttctccaat cccatagctt ccagcagcgg cagaagaaac
     3361 cgccccatgc agacttccgc tccgacatac agaaaaatcc ccaaaacggc catcgcaaat
     3421 accggcttct gcagcagctg gagactcgtg agaatgctgg gcggaacatc ggctttggtc
     3481 tcttcgattt tcaacgtggc aacccagaca aatgtaaccg ccataaggat aaagaaaatc
     3541 ggaaataccc cccgccagcc catactttca aaaagggcaa aacctgctat tgctgtaact
     3601 aaataggatg aaacggtact gccgattccc ttaaagcctt gggcaaagct gaggttgcgg
     3661 ccgtaagcgc cttgcggact gacatcccgc ataatggg
//
LOCUS       DAOTBO010000183         3694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20104_length_3694_cov_4.948337, whole genome shotgun sequence.
ACCESSION   DAOTBO010000183 DAOTBO010000000
VERSION     DAOTBO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20104_length_3694_cov_4.948337"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PLE88_12725"
     CDS             complement(<1..1422)
                     /locus_tag="PLE88_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77370.1"
                     /translation="MDVSRMVWSILWGIGLAAYPCRGGSPFVIQVVDEQTGRGVPMVQ
                     LETTSRIRLYTDSGGIAVFDEPGLMHQKVFFFVSSPGYEFPADGFGMRGAVLKTEPAA
                     AELLRIKRLNIAQRLYRITGQGIYRDSVLAGRDVPIRQPLLNSQVCGQDSANNCIYQN
                     KLYWFWGDTARIGYALGHFATAGATSIIAGPNADDPDKGINLNYFIDNDGFSKKMFPL
                     PQPGMVWIDGVMTARTAGGGQRMLCRFGLHKDLAEITLHGLGVFNDQTVCFEPILQEA
                     QSPFPYSTSGHAAAVETDAGFWWYCTIQFPASIRLRLRPEWEAAINPDSYEVFTALGQ
                     EAGLPSRWIAFGQLAGRFASRQAAQEALEKEHQSLRLYDIESGKPVVPHGGHVCWNPC
                     RNKWILICNQAGGDHSYLGEVWYAEADTPVGPWGYARKIITHDRYSFYNPKQHPYFAK
                     GSMIYFEGTYSFTFSADDRAATPR"
     gene            complement(1460..2179)
                     /gene="gloB"
                     /locus_tag="PLE88_12730"
     CDS             complement(1460..2179)
                     /gene="gloB"
                     /locus_tag="PLE88_12730"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOM77371.1"
                     /translation="MNPQEPVTVMEAGDNYIYLLDCGQDTAAAVDPSDAQAVLAHSRR
                     RQLTAVLATHHHSDHTGGIACLKKQTGCLVIGPGREHIPCLDRPAADGDVFQLGRLTV
                     RCIETPGHTTAGLCYLITTEAGLLLFTGDTLFICGCGRVLETDMETMFQSLQKLKALP
                     DETLVYPGHAYTEENIRFALTVEPDNRQLRQKLAAVCRLRRQNLPAVPSLLGEEKRLN
                     PFLRAADWQAFARLRKLKDAF"
     gene            2396..2635
                     /locus_tag="PLE88_12735"
     CDS             2396..2635
                     /locus_tag="PLE88_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77372.1"
                     /translation="MSSTVPGICCANCIYYEKTEENHGECHRYAPHPNSAQYVPKADS
                     VGQIKYDIHWPKVYDNNWCGQFGSRKKGEPAMPAE"
     gene            2699..3574
                     /gene="mtnP"
                     /locus_tag="PLE88_12740"
     CDS             2699..3574
                     /gene="mtnP"
                     /locus_tag="PLE88_12740"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOM77373.1"
                     /translation="MDKPMVGIIGGTGLGDVLAARIRNPVFKELDTPFGSPSGPVLVG
                     TFGHNQIAFLPRHGKGHRFHPSVVPYAANIFALKSLGVTALIASGAVGSLRQEIAPGH
                     LVLPDQFIDKTFRRQNTFFDSIGAVHVEFDQPCCRRLREKIMLAAAHIGSVTHADGTY
                     VCMEGPQFSTRAESLMHRAWGGDLIGMTALPEAKLAREAQMCYALVALVSDYDCWRPH
                     QPDQDKPGLLAEIIANLNRAADNAAALIEKTLEVNSGLCDQSCPCRKSLQSAVWTNPD
                     AVNPAARKLLNPLFE"
BASE COUNT          769 a         1106 c         1030 g          789 t
ORIGIN      
        1 gcggggcgtg gcggctctgt catctgccga aaaggtaaag ctgtaggtgc cttcaaagta
       61 gatcatgctg cctttggcaa aatacgggtg ctgcttggga ttgtaaaaag aatagcggtc
      121 gtgggtgatg attttgcggg cgtatcccca cgggccgacc ggcgtatccg cttcggcata
      181 ccagacctcg cccagatacg aatggtctcc gccggcctga ttgcagatca gaatccattt
      241 gttccggcag ggattccagc agacatgccc cccgtgcggg acgaccggct tgccgctttc
      301 gatgtcatac agccgcagcg actggtgctc tttttcaagg gcttcctgtg cggcctgtcg
      361 ggaagcaaac cggccggcca gctgcccgaa cgcaatccag cggctcggca gaccggcttc
      421 ctgccccagt gccgtgaaga cttcatagct gtcgggattg atggcggctt cccattccgg
      481 gcgcagacgc aggcgaatcg atgcgggaaa ctggatggta cagtaccacc agaagcctgc
      541 atcggtttca acagcggccg cgtgcccgct ggttgaatac ggaaagggcg attgggcttc
      601 ctgcaagatg ggttcaaagc agaccgtctg gtcattaaaa acccccagac cgtgcagcgt
      661 gatttcggcc aaatctttat gaagcccgaa ccgacacagc atccgctggc ctccgccggc
      721 ggtgcgggcg gtcatgaccc cgtcaatcca gaccattccc ggctgcggca gggggaacat
      781 ctttttactg aagccgtcgt tgtcgataaa gtagttcaga ttgattccct tatccgggtc
      841 gtcggcattc ggaccggcga tgatgctcgt agcgccggcg gttgcaaagt ggcctaaggc
      901 atagccgatg cgggcggtat cgccccaaaa ccagtacagc ttgttctgat agatgcagtt
      961 gtttgccgaa tcctgcccgc agacctgact gttcagcagc ggctggcgga tgggcacatc
     1021 acgcccggcc agcacgctgt cgcggtaaat gccctgtccg gtaatccggt acagacgctg
     1081 ggcgatatta agccgcttga tccgcagcag ttctgctgcg gccggttcgg tcttcaggac
     1141 agccccgcgc attccgaatc cgtctgccgg aaattcatag cccgggctgg aaacaaaaaa
     1201 gaagaccttt tgatgcatca gccccggttc gtcaaacacg gcaatgccgc cgctgtcggt
     1261 atagagccgg atgcggctgg tggtttcgag ctgaaccatc ggcacgcccc ggccggtctg
     1321 ctcatcgaca acctgaatca caaacggact gcccccacgg cagggataag ccgccagtcc
     1381 aataccccag aggattgacc ataccattct gctgacgtcc atctattgtc tccccaatgt
     1441 ccaaacccaa cctgttctgc taaaacgcat ctttgagctt gcgcaagcgc gcaaatgcct
     1501 gccaatccgc agcacgcaga aacggattga gccgtttttc ttcacccagc agcgacggta
     1561 cggcgggcag attctgccgc cttaaacggc agacggctgc cagcttctgc cgcagctgcc
     1621 gattgtccgg ctcgaccgtc agcgcaaagc ggatgttttc ttccgtgtat gcgtggcccg
     1681 gatataccag cgtttcatcc ggcagggctt tgagtttctg tagcgactga aacatcgtct
     1741 ccatgtccgt ttcgagcacc cgcccgcagc cgcagataaa cagcgtatcg cccgtaaaca
     1801 gcagcagccc ggcttctgtc gttatcagat aacagaggcc tgcggtggta tgccccggcg
     1861 tctctatgca tcgaaccgtc agccggccca gctgaaacac atcgccgtct gcagcgggcc
     1921 tgtcgaggca gggaatgtgt tcccgccccg gcccgatgac cagacagccg gtctgctttt
     1981 tgagacaggc gatgccgccg gtatggtcgc tgtggtggtg ggtggccagc acagccgtca
     2041 gttgccgccg gcggctgtgt gccaaaaccg cttgggcatc ggacgggtca acggcggcgg
     2101 ccgtgtcctg accgcagtcc agcaggtaga tgtaattatc ccccgcttcc atcacggtga
     2161 cgggttcttg cggattcatt gcactgcaat tgctcccgac ggtgtctgct ttttcaaaag
     2221 agcggggccg gtttccgctc atatcctttc tgcagaagta tacagcaatt ttggcgtact
     2281 ttaaactgct aaaatctttg cacttggctt caacagccgc tataataatt tccagcagcg
     2341 ctgaacaaaa cggttcgtaa cgctctttgg ctcatatcag gaaaggagtt aggcaatgag
     2401 ttcaacggtt ccgggaattt gctgtgccaa ctgcatttat tatgagaaaa cggaggaaaa
     2461 ccacggcgaa tgtcatcgct acgcgccgca tcccaattcc gcccagtatg ttcccaaagc
     2521 cgattcggta ggccaaatca aatacgatat ccactggccc aaggtctatg acaataactg
     2581 gtgcgggcaa ttcggctcgc gcaaaaaagg cgagcctgca atgcctgccg aataacaaca
     2641 ccgttaagcc ccggcggtct gtgcagatgc ctgccgggtt tgcaggagca gcgaatcaat
     2701 ggacaaaccc atggtcggca ttatcggcgg cacaggactg ggcgatgtct tggccgcacg
     2761 catccggaat cctgttttca aggagctgga tacgccgttc ggcagcccca gcgggcctgt
     2821 ccttgtcggc acattcggac acaatcagat tgcctttctg ccgcgccacg gcaaagggca
     2881 tcgctttcat cccagcgtcg tgccctatgc ggccaacatc tttgccctca agtcgctggg
     2941 cgtaacggct ctgatagcca gcggcgccgt cggctcgctg cgtcaggaaa tcgcccccgg
     3001 ccatctggtt ctgcctgacc agttcatcga caagacattc cgccggcaga acaccttttt
     3061 tgacagcatc ggggctgtcc atgtggagtt cgaccagccc tgctgccggc ggcttcggga
     3121 aaagattatg ctggcggccg ctcatatcgg ttccgtcacc cacgccgacg gcacctatgt
     3181 ctgcatggaa gggccgcaat tttcaacgcg ggctgaatcg ctgatgcacc gtgcttgggg
     3241 cggcgacctg attggaatga ccgccttgcc tgaagccaag ctggcacgag aggcacaaat
     3301 gtgctatgcg ctggtggcac tggtcagcga ttatgactgc tggcggccgc accagcccga
     3361 tcaggacaag cccggtttgc tggccgagat tattgccaac ctcaaccgtg ccgccgacaa
     3421 tgccgctgcg ctgattgaaa aaacgctgga ggtgaattcc ggcctctgcg accagtcctg
     3481 cccgtgccgc aagtcgcttc agtcggctgt ctggaccaat cccgacgctg tcaatccggc
     3541 cgctcgaaaa ctcttgaatc ccttgttcga ataaacccct gcatccttgc ggcaaggtct
     3601 gtcaaacgaa aacctctgct tgatattgtc tatccaaact gtccagatgg tcccaattct
     3661 gcatgcacta tcttgctatc ttcaaaaatc tgta
//
LOCUS       DAOTBO010000184         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20127_length_3690_cov_4.550757, whole genome shotgun sequence.
ACCESSION   DAOTBO010000184 DAOTBO010000000
VERSION     DAOTBO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20127_length_3690_cov_4.550757"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="cas7c"
                     /locus_tag="PLE88_12745"
     CDS             complement(<1..696)
                     /gene="cas7c"
                     /locus_tag="PLE88_12745"
                     /inference="COORDINATES: protein motif:HMM:NF016963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOM77374.1"
                     /translation="MSNVIQNRYEFVYLFDVENGNPNGDPDAGNMPRIDPETGYGIIT
                     DVCLKRKIRNYVQIIKNDQPPYCIYVKEKAILTNQQKRAYEALKIDPKGKTSDDIEKA
                     RLWMCQNFFDVRTFGAVMSLKDYNCGQVRGAAQLNFGRSIDVICPRELTITRMAVATE
                     KEAEAQSGDNRTMGRKNIVPYALYRTEGYVSACLAEQTGFNEEDLALLWDALINMFDH
                     DHSAARGKMCARRL"
     gene            complement(729..2450)
                     /gene="cas8c"
                     /locus_tag="PLE88_12750"
     CDS             complement(729..2450)
                     /gene="cas8c"
                     /locus_tag="PLE88_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOM77375.1"
                     /translation="MILQELCRYYDRLSANPDIAISPPGFSREKIHAEIVLDAAGNLL
                     QFNDLRVQRGKKLVPRDMIVPQSRKRTGAKPYETPFFLWDNTGFVLGSDKKNDEKTPL
                     KFQYFKEFHSDLLKGCSEPAAQALLRFLSRWNPGDAERLNNWEELAGGNVVFRLDGQR
                     TYLHDNSQLKKIWLEYTANQTSGQKGYCLISGQETAIARIHPAVKGVAGAQSSGAAIV
                     SFNDEAYESYGKKQNYNAPVGEAAAFSYITALNFLLASGSRQKIQIGDATTIFWTERD
                     SPIEGMFGMMLDPKDSDFDDNAELRRTLEAVREGKPLQGIEPDIKFYILGLSPNAARI
                     AVRFWYVCTVGQLEERIGQHFRDLRIQKSFDSEPDFPGVWRLLRETVNQKASDKSPQP
                     LLAGAVLRSIVEGTAYPQALQNAVINRIRADQEINYVRAAILKAVLVRKNRLYQQGME
                     VSMALDKENKNPAYLLGRLFAVLERAQQDALGAGINATIKDRFFGSASATPKVVFPQL
                     LRLAQHHIEKAEYGRMRDKQIEEILADLTEFPAHLSLDEQGLFAIGYYHQRQDFFKGK
                     EHSKQNQ"
     gene            complement(2447..3106)
                     /gene="cas5c"
                     /locus_tag="PLE88_12755"
     CDS             complement(2447..3106)
                     /gene="cas5c"
                     /locus_tag="PLE88_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOM77376.1"
                     /translation="MSFGIKLKVWGPYACFTRPEMKVERVSYDVITPSAARGILEAIY
                     WKPSIRWVIDRIHVFNPIRFMNIRRNEVASKIPLRNITTAMRGGPSSLELFIENDRQQ
                     RAAVILRDVDYLIEAHFEYVGDEDTNDGKHLDIFNRRARDGQCFMQPYLGCREFPAHF
                     RLLETDEPVPASPLRGRQDLGFMLHDIDYPDDMTPHFFRAEMIDGIIAIPRFQAKEVH
                     V"
     gene            complement(3127..>3690)
                     /locus_tag="PLE88_12760"
     CDS             complement(3127..>3690)
                     /locus_tag="PLE88_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77377.1"
                     /translation="CNREGLLECGQVFVFEPEEGLPGGYLRHSAQTAQGVIRRHSEDM
                     LSLEAIEEYFREYYWQQGAELDQKHILRLVREGGGRGDFPFRTIAEQFQMIPEENKPV
                     IIPREKEVQEIIEQSRHAESLRGVSRKLQPYTVQINPYHWSRLLEAGSLEIIQGMFPV
                     LRDIHLYDENTGLCCEYQNPEPESLIG"
BASE COUNT          955 a         1028 c          778 g          929 t
ORIGIN      
        1 taaccgacgg gcacacatct ttccgcgagc ggcagaatga tcatggtcga acatgttgat
       61 cagtgcatcc cacaacaaag ccaaatcttc ttcattaaaa ccagtctgtt cagccagaca
      121 ggcagacaca taaccttccg tacgataaag ggcgtacgga acgatgtttt tacgtcccat
      181 tgtccgatta tcgcccgatt gcgcctcggc ttctttttca gtagcaacag ccattcgcgt
      241 aatcgtcaat tcccgcggac agataacatc gatgctccga ccaaagttca gttgggccgc
      301 cccgcgaacc tgaccgcagt tatagtcttt taaagacata acggccccaa aagttctgac
      361 atcaaaaaag ttctgacaca tccaaagccg tgccttttca atatcatctg acgtctttcc
      421 cttcgggtcg attttcaagg cctcataagc tcgcttctgc tgatttgtca aaatcgcctt
      481 ttccttaaca taaatgcagt acggtggctg atcatttttg ataatctgaa cgtaattccg
      541 tatctttcgt ttcagacaaa catcggtaat aatgccgtaa ccggtttccg ggtcaatccg
      601 aggcatattg ccggcatctg gatccccatt gggatttcca ttttccacgt caaacagata
      661 aacgaactca taccgatttt gaatgacatt gctcatactg aaattctcct ttccgataga
      721 aaagattttt attgattctg cttcgaatgt tctttgcctt tgaagaaatc ctgccgctga
      781 tgatagtagc cgatggcaaa taagccctgt tcatccagag acagatgagc cggaaactcc
      841 gtcaaatccg ccagaatctc ctcaatctgc ttatcacgca ttcggccgta ttccgccttc
      901 tcaatatgat gctgagccaa gcgaagaagc tgcggaaaaa ccaccttcgg agttgccgaa
      961 gccgagccga agaagcgatc tttaatggtg gcgttgatgc ctgcccccaa ggcatcctgc
     1021 tgcgcccttt ccagaacagc aaagagccgc ccaagcaaat acgcaggatt tttgttctct
     1081 ttgtctaaag ccatagacac ctccattcct tgttgataga gtctgttttt tcgaaccagc
     1141 acagctttta aaatagctgc ccggacataa ttaatttcct gatcggcccg aatgcgattt
     1201 atcaccgcat tctgaagggc ctgcggataa gccgtgcctt ctacaatcga acgcagcaca
     1261 gcgccagcca gaaggggctg cggggattta tccgatgctt tctgatttac cgtctctctt
     1321 aataatcgcc agacaccggg aaagtcgggt tcagaatcaa aacttttttg aatccttaaa
     1381 tcccgaaaat gctgcccgat cctttcttcc agctgcccca ccgtacagac ataccagaac
     1441 cgaacggcaa tccgcgcagc attaggggaa agccccagaa tataaaactt gatatccggt
     1501 tcaattcctt gcagcggttt tccctctcgt acggcctcca gcgttcggcg aagttcagca
     1561 ttatcatcaa aatccgagtc ctttgggtcc agcatcatgc cgaacatccc ctcaatcggc
     1621 gaatcccgtt ccgtccagaa aatcgtcgtt gcatcgccaa tttgaatttt ctgcctgctc
     1681 ccggaagcca aaagaaaatt taaggcggta atataggaga aagccgcggc ctcgccgacg
     1741 ggagcattat aattctgctt tttcccatag gattcgtatg cttcatcatt aaaggagaca
     1801 atggccgcac ccgaggactg agcaccggcc acccccttga cggctggatg aatgcgggca
     1861 atagcagttt cctggccgct tatcagacaa tagcccttct ggccggatgt ttgattggct
     1921 gtatactcca gccagatttt cttcaattgg ctgttatcat gaagataggt cctttggccg
     1981 tcgagacgaa aaacaacatt gccgccggcc agttcttccc aattattcag ccgctccgca
     2041 tcccccggat tccagcgcga taaaaatcga agcagggcct gggccgccgg ttctgaacac
     2101 cctttcagca aatcagaatg aaactcttta aagtactgaa atttgagcgg cgttttttca
     2161 tcgttcttct tatcactccc aagcacaaaa ccggtgttat cccacaagaa aaatggtgtt
     2221 tcgtagggtt tcgcaccggt acgcttgcgg ctctgcggaa caatcatatc acgcggaacc
     2281 agtttcttgc ctctttgaac gcgcagatcg ttaaactgca gaagatttcc cgccgcatca
     2341 agaacaattt ctgcatgaat cttttcacgg ctgaacccag gcggagaaat ggcaatatcc
     2401 ggattggcgg acagccgatc ataataacgg cataattcct gaagtatcat acatgaacct
     2461 ccttcgcctg aaaccgcggg attgcaatga ttccgtctat catttctgcc cggaaaaaat
     2521 gcggcgtcat atcatcagga taatcaatat catgcagcat aaaaccgaga tcctgcctgc
     2581 cccgcaaggg agaagcggga acaggttcat cggtttcgag aagtcgaaaa tgggccggaa
     2641 actcacggca ccccagatag ggctgcataa aacattgccc gtcccgcgcc cgccgattga
     2701 agatatccag atgtttgccg tcattggtgt cttcatcacc gacatactca aaatgggcct
     2761 caataagata atccacatcc cgcagaatca ccgcagcacg ctgctggcgg tcattctcaa
     2821 taaacaactc cagtgaagac ggcccacctc tcatagctgt cgtgatattt cgcagaggga
     2881 ttttgctggc cacctcattg cgccggatat tcataaagcg aataggattg aaaacatgaa
     2941 tccggtcaat aacccagcgg atagatggtt tccaatagat ggcctccaga ataccccgcg
     3001 cagctgatgg cgttatcaca tcataactga cccgctccac cttcatctcc ggccgggtaa
     3061 agcaggcata gggaccccac accttcagtt taattccgaa actcatattg tctcctttcc
     3121 ataaaattat ccaatcagtg actccggctc cggattctga tattcgcagc acaagccggt
     3181 attttcatca tacaaatgaa tatctctgag caccggaaac atcccctgaa ttatctcaag
     3241 gctgccggcc tccagcagtc tgctccaatg ataaggattg atttgaaccg tgtacggctg
     3301 caattttcga ctcacccccc tcagagactc agcgtgccgg ctttgctcga taatttcctg
     3361 cacctccttc tctcggggaa tgattaccgg cttattctct tccggaatca tttgaaattg
     3421 ttccgcaata gtccgaaacg gaaaatcccc tcttccgcct ccttctcgaa cgagcctcag
     3481 aatatgtttt tggtcgagct ccgccccctg ctgccagtaa tattccctga aatattcctc
     3541 gatagcttcc agggaaagca tatcttcaga atgacgccgg ataacccctt gtgccgtttg
     3601 cgctgaatgt cgaagataac cgcccggcag accttcctcc ggctcaaaga caaaaacctg
     3661 gccgcattcg agcagtcctt cccggttgca
//
LOCUS       DAOTBO010000185         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20297_length_3665_cov_5.232687, whole genome shotgun sequence.
ACCESSION   DAOTBO010000185 DAOTBO010000000
VERSION     DAOTBO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20297_length_3665_cov_5.232687"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLE88_12765"
     CDS             complement(<1..1007)
                     /locus_tag="PLE88_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOM77378.1"
                     /translation="MRKAGRQKWAVWLLLAFLPAVWADTYYLSSESGWQTTDSPEGKY
                     LLSMSKLKQQLLTGKPAEAAAALEQIKTDFPDMAGKEIDSYIAAEKLYAAAKWDKAAA
                     LYKKFIDAWPDSILQPAAMERIYAIGAAYLQGHKRTFLKVLKLPAFDTGADLMRDVAD
                     RAGQAPIALRALTTLAENQERREKFLDAYQTWSEIKDRWPTGEVGRDALLRMARALHA
                     CYKGTEYDASVLPSAATYYEQYNGRYPDQAAQLEIPAVLDTIEQQQAYKLYATGFYYE
                     RTGRPEAARKYYTKVVTAQPDTAAAQMAQARLAPDAPPAVKKTLRRRTVGLTERFLDS
                     W"
     gene            1231..1551
                     /locus_tag="PLE88_12770"
     CDS             1231..1551
                     /locus_tag="PLE88_12770"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOM77379.1"
                     /translation="MVEKEYELLSDEVLEKAADCLKVMGHTARLRIVNLLMQGEYSVN
                     QIAQLCRLHPNQACEHLRLMKNCGYLTSRRAGRTVYYQIASPQLPGLIECIRLHCRPE
                     QPVQ"
     gene            1591..>3665
                     /locus_tag="PLE88_12775"
     CDS             1591..>3665
                     /locus_tag="PLE88_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOM77380.1"
                     /translation="MSESKRIVIIGGVAGGASAAARLRRLDETAHIVLLERGPYISFA
                     NCGLPYHIGNVIPDRNRLLVQTPEGMSSRFRIDVRTNSQALRIDRDKKEVLVANLTDG
                     RRYLLPYDYLILSPGAEPVRPPIPGADLPEVFVLRTMSDMDAIKKAVDQQQVEQAVVV
                     GGGYIGLEMAEALTERKVNVALVELEGQVMGTIDPEMAAPLHQELQMHGVKLYLNTSV
                     TEICRQADGLEAVLSSGDRLAAQMVLLAVGVRPESKLAKDAGLAIGPRGGIAVNSQMQ
                     TSDPCIYAVGDAVETAEFVTGAEAVIPLAGPANRQGRIAADNICGRSSVYRRTQGTAI
                     CKVFNLAAAVTGLNEKALKRAGLPYEKVYVHPASHAGYYPGAMQMSLKLLFDPADGRI
                     LGAQAVGADGADKRIDVIATAMRAGLTVQQLQDLELCYAPPYGSAKDAVNYAGFAAAN
                     VLNGDVRLFHFSDAVNPPADALLLDVRTLPEVQAGTIPGAAHIPLDQLRSRLGELPKD
                     KEIWAFCQVGLRGYLACRILSQHGFRCRNLSGGYKTYAAALGIRQQSRPVPQEIKSDT
                     GQEETKPKAAESTVRIDACGLQCPGPILRLKETIDQLAAGQTVEIVSTDAGFAADIRG
                     WCASTGSRLLELNARNGTYTARIEKQAAAGLPGADAKNKTLIVFSGDFDRAMAAFIIA
                     NGAAAMGSRV"
BASE COUNT          755 a         1040 c         1119 g          751 t
ORIGIN      
        1 aaccacgagt ccagaaaccg ctcggtcaga ccgaccgtcc gccgccgcag tgtcttcttg
       61 accgccggcg gggcatcggg cgccagacgg gcctgagcca tttgggctgc ggcggtgtcc
      121 ggctgagcag tcactacttt ggtgtaatac tttcttgcgg cctccggccg gccggtgcgc
      181 tcgtaataaa agccggtcgc atagagcttg taggcctgct gctgctcaat cgtatccagc
      241 acagcgggga tttccagctg cgctgcttgg tccggatagc ggccgttgta ctgctcataa
      301 taggtggccg cactcggcag taccgaggca tcgtactctg ttcctttgta gcaagcatgc
      361 agcgcccggg ccatccgcag cagggcatcg cggccgactt caccggtcgg ccagcggtcc
      421 ttgatttccg accatgtctg ataggcatcc agaaacttct cgcggcgttc ctgattttcc
      481 gccagcgtgg tcaaagcccg cagggcaatg ggggcttgac cggcgcggtc ggccacatcc
      541 cgcatcagat cggcgccggt atcgaaggcc ggcagcttga gaaccttcag gaaggtgcgc
      601 ttgtgcccct gcagataggc cgccccgatg gcataaatcc gctccatagc cgccggctgg
      661 aggatgctgt ccggccaggc gtcaataaac ttcttgtaaa gcgcagcggc tttatcccat
      721 ttggccgccg cataaagctt ctcggcagca atgtagctgt caatctcttt gccggccata
      781 tcgggaaaat ctgttttaat ctgttccaga gccgccgccg cttctgccgg cttgccggtc
      841 agcaactgct gcttgagttt cgacatcgac agcaggtatt tgccttccgg cgaatccgtt
      901 gtctgccagc cggactcgct ggagaggtaa taggtgtcag cccagactgc cggcaaaaaa
      961 gccagcagca gccatacggc ccatttctgt cttcctgctt ttctcataaa gtccttcttc
     1021 tttatccagt gaatagaaaa tcaaacgatt gtctgacccg ctgattatgt tccctattat
     1081 accgcaaaaa aacggcctgc aaacaccctt tatccctttt tttcttaaca gacttcggca
     1141 aatcgagcaa aaattatatg gaaatatcga aaccttacga tatattgttt cgtatagttt
     1201 gtcaacagca aattttgaaa ggcgatatca ttggtcgaaa aggaatacga attgttatcg
     1261 gacgaagtgc tggaaaaagc cgctgactgc ctgaaagtca tggggcatac tgcccgcctg
     1321 cggatagtga atttgttgat gcagggggag tattcggtca accagatagc ccaactttgc
     1381 cgcctgcatc ccaatcaggc ctgtgagcat ctgcggctga tgaaaaactg cgggtatctg
     1441 accagccggc gtgcaggacg gacggtgtat tatcagattg ccagtccgca gctgccgggg
     1501 ctaattgaat gcattcggct ccattgccgg cctgaacagc ccgtccaata aagaatccga
     1561 taaagaaaaa gaacatctta aggagattgt atgtctgaat cgaaacgcat tgttatcatc
     1621 ggcggcgtgg ccggcggcgc atcggcggcg gctcgtctgc gtcggctgga cgaaacagcc
     1681 cacattgttc tgctggagcg ggggccgtat atttcctttg ccaactgcgg tctgccgtat
     1741 catatcggca atgtcattcc cgaccgcaac cggctgctgg ttcagacgcc cgaaggcatg
     1801 agcagccgct ttcggattga tgtccgcacc aacagtcagg ccctccggat tgaccgcgac
     1861 aagaaagaag tgctggtggc caatctgacg gatggccgcc gctatttgtt gccgtacgat
     1921 tatctgattt taagccccgg cgccgaaccg gtgcggccgc cgataccggg agcggatctg
     1981 ccggaagtct ttgtgctgcg gacgatgtcg gatatggacg ccatcaagaa ggcggtggac
     2041 cagcagcagg tagaacaggc cgtggtggtc ggcggcggct atatcggtct ggaaatggcc
     2101 gaagcactga ccgaacggaa agtgaacgtc gcactggtgg aactggaagg acaggtgatg
     2161 ggcacgattg acccggaaat ggccgccccg ctgcatcagg agctgcagat gcacggcgtc
     2221 aagctgtacc tcaataccag cgttacggaa atctgccggc aggccgacgg tctggaagct
     2281 gtcttaagca gcggcgaccg gctggcggcg caaatggtgc tgttggctgt gggcgttcgg
     2341 ccggagagca agctggccaa agacgcaggc cttgctatcg ggccgcgcgg aggcattgcc
     2401 gtcaattcgc agatgcagac cagcgatccg tgcatttacg ctgtcggcga tgcggtggaa
     2461 acggcggagt ttgttaccgg tgcagaggcc gtgataccgc tggccggtcc tgccaaccgg
     2521 cagggacgga ttgccgccga caatatctgc gggcgcagca gcgtctatcg ccgcacgcag
     2581 ggcacggcta tttgcaaggt tttcaatctg gccgcagcgg ttaccggcct gaacgaaaaa
     2641 gccctgaaac gggccggcct gccgtatgag aaagtctatg tgcatccggc cagccacgcc
     2701 ggctattatc ccggtgccat gcagatgagt ttgaagcttt tgttcgaccc ggctgatgga
     2761 cgcattctgg gcgcacaggc cgtcggggca gacggggcag acaagcggat tgatgtcatc
     2821 gccacagcca tgcgtgcggg attgacggtc cagcagctgc aggatttgga gctgtgctat
     2881 gccccgccgt acggctcggc caaagatgca gtcaattatg ccggctttgc cgccgccaat
     2941 gtgctcaacg gcgatgtgcg gctgttccat ttcagcgatg cggtcaatcc gccggccgat
     3001 gccctgctgc tggatgtgcg gactctgccg gaggtgcagg ccggaaccat tcccggggcg
     3061 gcacacattc cgctggatca gctccgcagc cggctggggg aactgcccaa agacaaggaa
     3121 atctgggcgt tttgtcaggt gggcttgcgg gggtatctgg cctgccggat tttgtcgcag
     3181 cacggctttc gctgccggaa tctgtccggc gggtacaaga cctatgcggc cgcactgggg
     3241 attcgtcagc aatcccggcc ggtgccgcag gaaatcaaga gcgataccgg tcaggaggag
     3301 accaaaccca aagcggccga aagcacggtg cggattgatg cctgcgggct gcagtgtccg
     3361 ggaccgatcc tgcggctgaa ggaaaccatt gaccagcttg ccgccggtca gacggttgag
     3421 attgtcagca ccgatgccgg ctttgccgcc gacatccgcg gctggtgcgc cagcaccggc
     3481 agccggctgc tggaactgaa cgcccgcaac ggcacttaca ccgcccgcat tgaaaaacag
     3541 gccgccgcag ggctgcccgg ggccgacgcc aagaacaaaa cgctgattgt cttcagcggc
     3601 gattttgaca gggcgatggc ggcgtttatt attgccaacg gcgccgcggc gatgggcagc
     3661 cgggt
//
LOCUS       DAOTBO010000186         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20366_length_3656_cov_4.576507, whole genome shotgun sequence.
ACCESSION   DAOTBO010000186 DAOTBO010000000
VERSION     DAOTBO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20366_length_3656_cov_4.576507"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1429
                     /locus_tag="PLE88_12780"
     CDS             422..1429
                     /locus_tag="PLE88_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77381.1"
                     /translation="MQKPNELTQLLVKMIQLMQLDNPIGHRNLTLVPIKGPSSQLDYI
                     LGAEAIAAGTLTVQEISESGAVNTLTVENKGDRRVLLLDGEELIGAKQNRILNTTLLI
                     EAHTTQKIPVSCVEQGRWRHISHAFAVGVYSPPEMRSKKSRAVSSSYIRCGQADSNQV
                     EVWREVDKLCCDLRANAPTRAMKDVFDQRANDCNSYIRALPYPEGVRGVMAAVSGRFA
                     AMDVLDKPAAFQRIWNRLVSGYAMDALRRKDKEHKPFTEKSAQFIMDSISDCQVSVFD
                     AVGLGQELRFESPLILGQALVVQDCLVHISVFPNEANSRPDDPEYRIMPPSFRKRPRR
                     P"
     gene            1488..2486
                     /locus_tag="PLE88_12785"
     CDS             1488..2486
                     /locus_tag="PLE88_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HOM77382.1"
                     /translation="MAHNLEIKNGQASMFYVGQPPWHHLGTQLERPAIAKEAIEAAKL
                     NWQVTKAPLYVHINGSPTQVHNKYGVVRKDLCDTKAPPILGIVGEQYTPLQNSEAFDF
                     FDPIVGKDAAVYHTAGVLGHGERIWILAKLPADIRVIGDDIVNKYLLLSNSHDGLSAV
                     QVKFTPIRVVCQNTLTMALSQGPTIRIVHTKDMKQRLKQAEQLLGIVHTHYRDIEADF
                     AAMTRVQVDGERLKEYLSQVFPDPKDPDNQKALKRLRDDRSLAEYFFDRGKGTDIKGV
                     RGTLWAAYNGVAELIDHRVVGFSETRRLGSIWFGDGYLTKARAYKVAKEKMRAWQN"
     gene            2712..>3656
                     /gene="cas6"
                     /locus_tag="PLE88_12790"
     CDS             2712..>3656
                     /gene="cas6"
                     /locus_tag="PLE88_12790"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOM77383.1"
                     /translation="MSEKISLDKAAIQIASVRLSGYFLSEGRLPAFRGATLRGGLGFH
                     LRRTVCNNLKRSCQDCFLAEECAYSNFFDGILPKDRNFMKLYDNVPQPYLLVLPLEEP
                     ENIHKEDPLCFTMKIFGKASELFPYVAYSFIQMGEKGLGKDKIPFEISRIEQPGGEVL
                     YEKSVNRIMKPKPQIAAYDIMPIDCNDPLEIRFFTPVRIRKNGQTLKTIRFEDLIEAA
                     LRRITIMNYFYGTGQIVDNEKIKAMIEHAKSVEKVNDATEYYSFRRYSGRQERTIELD
                     GLTGTAVFKKVTPELAGILKFAQTIGLGKSTSFGFGNIE"
BASE COUNT          942 a          945 c          958 g          811 t
ORIGIN      
        1 ttccctcata gggagtctgc ccccatcggc ggcatcggac agatagagtc gccatgcccg
       61 ctgattggca gcagtattca gcttgaactg gccaatatct tagaactgag acgccggcct
      121 gcaggacttg atacccttgc cgaaatgctg tattgccgat gagatggccc ccgacattaa
      181 tcccttggcc cactgtcatt gggctgcacg aaaaaaatta aaaaaataca aatttttccc
      241 tttcccttat caccctacat atagccccgt cattcccccg gaagccccac ttcttgtagg
      301 tctgaccagc cctgtcatcc ccttggcttg gtctttctta aaaaggttct gaccatttat
      361 gtcatacccc cgctgtatca tttagggtga caagaagatt ttttttaaga agcagtaagc
      421 catgcagaaa ccaaacgaac tgacgcaatt actggttaaa atgatccaac tgatgcaact
      481 ggataatccc attggacacc gcaacctgac ccttgttccc atcaaaggtc catccagcca
      541 actcgattac atcctcggcg ccgaggctat cgcggccggc acactgacgg tccaagagat
      601 cagcgaaagc ggagcggtca acaccctgac cgtcgagaac aaaggcgaca ggcgggtatt
      661 gcttctggac ggcgaggaac tgatcggggc caagcagaac cgcatcctca acacaaccct
      721 cctcatcgag gcccatacca cccagaaaat acctgtctcc tgtgtcgaac agggacgttg
      781 gcggcatatc tcgcacgcat ttgccgtcgg ggtgtactcc ccgcccgaga tgcggagcaa
      841 aaagagccgc gctgtaagct caagctatat ccgctgcggg caggcggaca gcaaccaagt
      901 cgaggtctgg cgggaagtcg acaaactctg ctgcgatctt cgtgcaaacg cccccacccg
      961 ggcaatgaaa gatgttttcg accagcgggc caacgactgt aacagttata tccgggctct
     1021 gccctatccg gaaggcgtcc gcggcgtcat ggcggccgtt tcaggccggt tcgctgccat
     1081 ggatgttctg gacaaacccg ctgcgttcca gcggatctgg aaccggctgg tcagcggcta
     1141 tgccatggat gccctccgcc gcaaggataa agagcataag ccgtttacgg aaaaaagtgc
     1201 acaattcatt atggacagca tttctgactg tcaggtctcg gtatttgatg ccgtcggact
     1261 gggtcaagag ctgcggtttg aatcccccct gatattgggg caggcgctgg tcgtccagga
     1321 ctgtctggtc cacataagcg tatttcccaa tgaagccaac agccgcccgg atgatccgga
     1381 atatcggatc atgccccctt cattccgaaa gcggccgcgg cggccttgat gcagtcagcc
     1441 aagaaaccgc caaaaaaccg gattgtaaag agttgaaagg agcaattatg gcgcataatc
     1501 tggagatcaa aaacgggcaa gcatcgatgt tctatgtcgg ccagccccct tggcatcatt
     1561 tggggaccca gctggaacgg ccggccatcg ccaaagaggc aatcgaggcc gctaagttaa
     1621 actggcaagt cacgaaagcg ccgctgtatg ttcacattaa cggcagtccg acccaagtgc
     1681 ataacaaata cggcgtggtc cgaaaagacc tgtgcgatac gaaagccccc ccgattttgg
     1741 gaattgtcgg cgagcagtat accccgctcc aaaacagcga ggcctttgac ttctttgacc
     1801 ccatcgttgg caaggatgct gcggtctatc atacggccgg ggttctcggt catggcgaac
     1861 ggatctggat tcttgccaaa ctgccggccg acatccgggt catcggcgat gatatcgtca
     1921 acaagtacct gctgctgtcc aacagccacg acggattaag tgctgtacag gtcaaattca
     1981 cacccatccg tgttgtctgt caaaatacct tgacgatggc cctcagtcag gggcccacca
     2041 tccggatcgt ccataccaaa gatatgaagc agcggctgaa acaggccgaa cagctgctgg
     2101 gcatcgtgca cacgcattac agggatatcg aagcggattt tgctgcgatg acccgcgtgc
     2161 aggtggacgg agagcggctg aaagagtatt taagtcaggt attcccggac cccaaagacc
     2221 ccgataacca aaaggcctta aaacgcctcc gggacgaccg ctctttggca gaatactttt
     2281 ttgaccgcgg caagggaaca gatatcaagg gtgtccgggg aacgctctgg gctgcataca
     2341 acggcgtggc cgaactgatc gaccaccggg tcgttggttt ctcggagacc cgcaggctgg
     2401 gttcgatctg gttcggggac ggttatctga caaaagcccg tgcctacaaa gtggccaaag
     2461 agaagatgcg ggcatggcag aattaaccgg aatacagcag gaatgcccgt ggggaagaga
     2521 caggatttcc atcgttcttt gatggttctc caataggtaa cggatttttt gttgcatttt
     2581 atcaagattg ggttagaatc agaacacata tagtataaga agcagaagat attcaatggg
     2641 ctgcagaaag gttcgatttc acagtttgtt cgtaggattt gtctttggag attgagggcg
     2701 gagaagcacg cgtgagcgaa aaaatatcct tggataaagc ggctattcag attgccagcg
     2761 tcagactatc gggatatttc ttgtctgaag gccgtctgcc ggcctttcgc ggggctaccc
     2821 tgaggggggg acttggattc catctgcgaa ggactgtatg caataatctc aagcggtcct
     2881 gccaagactg ttttctggcg gaagaatgcg cgtattccaa cttctttgac ggaatacttc
     2941 ccaaagaccg gaactttatg aagctctatg acaatgtacc ccagccatat ctgctggtgc
     3001 ttccattgga ggagccggaa aatatccata aagaagatcc cctttgcttt accatgaaga
     3061 tttttggcaa agcgtcggag ctgttcccgt atgttgctta cagttttata caaatgggtg
     3121 aaaaagggct tggaaaagac aaaattcctt ttgaaatcag tcgcattgaa cagccggggg
     3181 gagaggttct ctatgaaaaa agtgtaaatc gcatcatgaa gcccaagcct caaattgcgg
     3241 cgtacgatat catgccgatt gattgtaatg atcctttgga aatccgtttt tttacccccg
     3301 tccgtattcg gaagaacggg cagaccctca agactattcg ctttgaggat ctgatagaag
     3361 cagcccttcg ccgcataacg attatgaact acttttacgg aacagggcag attgtcgaca
     3421 atgagaaaat caaggcgatg atagagcatg ccaagtctgt cgaaaaggtc aacgatgcca
     3481 ccgagtatta ttcgtttcga cggtattccg gcaggcagga acgcaccatc gaactggacg
     3541 gcttgacggg cacggcagtt ttcaaaaagg tgacgccgga attggcgggg attctgaaat
     3601 ttgcccaaac aatcggcctg ggcaaatcaa ccagcttcgg attcggaaat attgaa
//
LOCUS       DAOTBO010000187         3647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20433_length_3647_cov_3.977728, whole genome shotgun sequence.
ACCESSION   DAOTBO010000187 DAOTBO010000000
VERSION     DAOTBO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20433_length_3647_cov_3.977728"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLE88_12795"
     CDS             <1..200
                     /locus_tag="PLE88_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77384.1"
                     /translation="DIWIYVNGEPVIYRAGWTTDYIVVPVPADKLRLFKTGRNIIAVT
                     CKQTSGGQGVDVGIMNIKKNR"
     gene            308..2338
                     /locus_tag="PLE88_12800"
     CDS             308..2338
                     /locus_tag="PLE88_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM77385.1"
                     /translation="MGIIDAIVFVLFVLGVIGTGLWKSRNEKSQEDYFLAGRGLKWWL
                     IGFSLIAANISSEQFVGMSGNAASHVGLAIASYEWMAAIVLAIVAFFFLPYFLKAGIY
                     TMPEFLEYRYNQWARLIMAVSMLLIYMLLLGAVTYSGALTINTMAGEMSIAWVPSVAS
                     ASVFIGLIAMIYVATGGLKACAWADLIQGSALIIGGGIIMVYAFKMLGNVSEAAYITD
                     VEKGTIAVKTFTPDQSIMERFLDLNRVRLNMFLPRNDAVLPWTALLLGLWIPNFYYWG
                     LNQYITQRTLGSASLAEGQKGIIFSAFMKLLIPFVIVVPGMIAFNLFSSELKCKTVSD
                     NGLVIAKYKMANPESSLVQVVDSPSRDTLETWKDGVFMIAICPNQDTITKLHNKSPYI
                     LPVTKDSYENLKIAQYTVFTSDDKSWDEAFPSLAIELQRYNEATRKAAIDNHAAVSIE
                     KLIGYKYDAALGQLLSKVLPENIGLAGFVLAALLGAVVSSLAAMLNAASTIFMMDIYT
                     RYINKQASKKTIVTLGRVSVLLFSLIGISIAPLLGNPKISHSIFMIIQESQGFIAPGI
                     LAIFVIGLLVPQAPRCSGVIGLLTSIVSYGLLKVAVPQLQFVNRMAVCFTICLAAIGM
                     ITMIKPLPTFIPFRKNTNIELKSSKSAIVAVFFVVLLTIMLYVIFSPAGIAR"
     gene            complement(2472..>3647)
                     /locus_tag="PLE88_12805"
     CDS             complement(2472..>3647)
                     /locus_tag="PLE88_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOM77386.1"
                     /translation="VAVTTGQYPARWKIYSYLDSRSANRRRNMADFLSPLAPSLARQL
                     KTAGYATAHFGKWHMGGGRDVDDAPHPQTFGFDESSVTFEGLGDRLLINNDNLSKQSA
                     RLQQGQITWVEKHQISKIRVDQTLDFIRRQQGRPFYVNLWFNDVHDPHMPEPGQIENY
                     KDISNNPYEQKFYAVLAEMDRQIGRVLDGLAQMGLEEKTLVILTSDNGPTDWPYYYKE
                     GFLPPGSVGPFNGRKWSLYEGGIRMPFIVRWTGTIPAGKVDSSTVACGIDLLPTLCKL
                     AGAGLPEGAVQDGQDISSSFLGTPVERKHPIFWYYPNQPKPGKSEHISPTLAVRNGRW
                     KLLADLDGSKMQLYDLAEDLGEKTNLLSEHPETAQRLWNQVSSWAESMGLKTEKSEP"
BASE COUNT          927 a          858 c          875 g          987 t
ORIGIN      
        1 aagatatttg gatttacgtc aacggtgaac ccgtgatata tcgagctggc tggactacgg
       61 attatattgt tgtgccggtt cccgccgata agcttcgttt attcaagacg ggtcgaaata
      121 tcattgccgt cacctgcaaa cagaccagcg gcggccaagg tgtagatgtc ggcattatga
      181 acataaagaa aaaccgctga ccagcaaaat taacccgtga atcgctttta aggccgcatt
      241 ggccttgaca gtgtgctgtg agttccatta ttgagaagcg attataaacg gacgagggaa
      301 aatattcatg ggaatcatag atgccatcgt attcgtatta tttgtgctcg gcgtcatcgg
      361 cacaggctta tggaaaagca gaaatgagaa atctcaggag gattactttc tcgcagggcg
      421 cggactgaaa tggtggctga ttggtttttc gcttatcgcc gcaaatattt cttccgagca
      481 atttgtgggg atgagcggca atgctgccag ccatgtcggt ctggccatcg caagttatga
      541 gtggatggcc gctattgtac ttgctattgt tgcgttcttt ttcttgcctt attttctcaa
      601 agccggcatt tatacgatgc cggaatttct ggagtatcgc tataatcaat gggctcgact
      661 gatcatggcg gtatcgatgc tgctgattta tatgctgttg ctgggagcgg tcacctattc
      721 cggcgcatta acaattaata cgatggccgg agagatgagt attgcatggg ttccttccgt
      781 tgcctctgca tctgtattca tcggcttaat tgcaatgatt tatgtggcca caggcggctt
      841 aaaggcgtgc gcctgggcgg atcttatcca gggcagtgcg ctgattattg gtggcggcat
      901 tattatggtg tacgccttta agatgctggg aaatgtgtca gaggccgcat atatcacaga
      961 tgttgaaaaa ggaacgattg ccgtaaaaac gttcaccccg gaccaatcca tcatggaacg
     1021 ttttttagac cttaatcggg tgcggctaaa tatgtttctg ccccgaaatg acgccgtcct
     1081 gccctggacg gcattgctgt tgggtctgtg gataccgaat ttctactatt ggggccttaa
     1141 tcaatatatc acgcagcgaa cgctggggtc ggcgtcacta gccgaaggtc agaaaggcat
     1201 cattttctct gcgttcatga aacttctcat tccatttgtg atcgttgtcc cgggaatgat
     1261 tgccttcaat ctgttctcct cggaattgaa atgtaagact gtcagtgata atggattggt
     1321 aattgcaaaa tataaaatgg caaatccgga atcctctctg gtccaggtcg tcgattcccc
     1381 ctcaagggac accctcgaga cttggaagga tggcgtgttt atgatagcta tttgtcccaa
     1441 tcaggacacc ataacaaaat tgcataacaa aagtccctat attctgccgg tcacaaaaga
     1501 cagttatgaa aacttgaaga ttgcacaata cacggtgttt acatctgatg acaaatcctg
     1561 ggacgaagca tttccttcac tggcgattga attgcagaga tataatgaag caacccgcaa
     1621 agccgcgatc gataaccatg cggcggtaag tatcgaaaaa ctgattggct ataaatatga
     1681 tgcggcattg gggcaattgc taagtaaagt ccttcctgaa aatatcgggt tagccggatt
     1741 tgtattggcc gctttattgg gggcagtcgt cagttcgctt gcagcaatgc tgaacgcggc
     1801 ttccaccatc tttatgatgg acatttacac gaggtacatc aataaacagg cgtcaaaaaa
     1861 aacaattgtt accctaggca gagtcagcgt acttcttttt tctttaatcg gcatatccat
     1921 tgctccctta ctcggaaatc caaaaatcag ccatagcata ttcatgatta ttcaggagag
     1981 tcagggcttc atagctccgg gcatccttgc tatttttgtc attggattat tggtaccgca
     2041 ggccccaaga tgcagcggcg taatcgggct gctcacgagc attgtttctt acggattgct
     2101 gaaagtggct gtcccccagc ttcaatttgt aaatcgtatg gctgtgtgtt ttacaatctg
     2161 cctggctgcc ataggaatga tcaccatgat aaaaccgcta cccaccttca tcccattcag
     2221 gaaaaacacg aatattgagt taaaatcatc gaaaagtgcg atagtggcag ttttttttgt
     2281 cgtactgctg acaattatgt tatatgttat attcagtccg gcaggcattg ctcgctaatt
     2341 aaaaagtccg gcaattctcc ggggagccag aaatatattg tcctgccgct tccgaaagca
     2401 cacatttttt atccattgtt tagcaaccta aaaatggcaa aagtcgagat gagaatctcg
     2461 accggccaag gtcacggttc agatttctcg gtcttcaggc ccatggattc ggcccaggaa
     2521 ctaacttgat tccacaggcg ctgtgccgtt tcgggatgct cgctgagcaa attagtcttc
     2581 tcccccagat cttcagccag atcataaagc tgcattttac tcccatccag atcggctaac
     2641 agtttccacc ggccattccg aaccgccagc gtcgggctga tgtgttccga cttgcccggt
     2701 ttgggctgat taggatagta ccagaaaatc ggatgttttc tttctaccgg agtccccagg
     2761 aaagagctgc tgatatcttg accatcctga acagctccct ctggcagacc ggcacccgca
     2821 agtttacaca gggttgggag cagatctata ccacaagcga cggtagagct gtccaccttt
     2881 ccggccggaa tcgttcctgt ccagcgaaca ataaagggca tgcgtatgcc cccttcgtac
     2941 agactccact tgcggccatt gaagggacct acgctgccgg gaggcagaaa accctcttta
     3001 taataatacg gccaatcagt cggtccatta tcactggtca gaattaccag ggttttctcc
     3061 tccaatccca tttgcgccag gccatccagt acgcgtccga tctggcgatc catttcagcc
     3121 aagacggcat aaaatttctg ttcataagga ttgtttgata tatctttata attttcaatc
     3181 tgccccggtt caggcatatg aggatcgtgg acatcgttaa accagagatt gacataaaat
     3241 ggccggccct gctgccgccg aataaaatcc aacgtctgat caacgcgaat ttttgaaatc
     3301 tggtgcttct ccacccaagt aatttggcct tgctgaaggc gggcactttg tttggaaagg
     3361 ttgtcgttgt tgatcagcag acgatctccc aatccctcga aagtaactga agattcatca
     3421 aaaccgaagg tctgaggatg aggggcatca tcaacatcgc ggccgccccc catgtgccat
     3481 tttccaaaat gggcggtagc atacccagcc gtcttcagtt gtcgggccag agacggcgct
     3541 agcgggctga gaaaatctgc catgttccgg cgtcggtttg cactgcgaga atcaagatag
     3601 gaataaattt tccaccgcgc aggatactgg cctgtagtca cggcaac
//
LOCUS       DAOTBO010000188         3632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20541_length_3632_cov_4.321219, whole genome shotgun sequence.
ACCESSION   DAOTBO010000188 DAOTBO010000000
VERSION     DAOTBO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20541_length_3632_cov_4.321219"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1777)
                     /locus_tag="PLE88_12810"
     CDS             complement(<1..1777)
                     /locus_tag="PLE88_12810"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOM77387.1"
                     /translation="MAQINPTKLKPTDLTRLLNSAGFGEVLNERTLRRHRNRAGYTIG
                     DARTVNLFQYAAWLTQQYLAPPKESRNYDQIREAARLRNAELARAGQDIGHIPAVVNP
                     DRKTKAMASFKFFCDAYFPDVFYLTWSADHHKVIAKIELAVLKGGLFALAMPRGSGKT
                     VLMQMACLWSALIGATEFVCLIAASADRAKNLLENIKVWLETNERLHEDFPEVTLPIL
                     ALERITNRQKGQKHNGVSTRIEWAADKIVLPTIEGSTASGVVISCSGMKGSDIRGQNH
                     ARADGKVVRPQLVMVDDPQTTESAWSPSQSQRREAILAGDVLGMAGPGKKISGLMACT
                     VIRPGDMADNILDREKHPEWQGERTKMVYVFPTNEKLWAQYAEIRADSLRNDGDGSQA
                     TEFYRENREAMDAGSVIAWPERHNIDELSAIQHAMNLRLRDEAAFFAEYQNEPVVETE
                     GEEMLNADQIVAKLNGYNRGVVPISCHNLTMFVDVQQKALFWMLCGWETDFTGYILDY
                     GTWPEQKRLYFTLRDIRRTIAQQKPGAGLEGAIYHALQQLTEEKLACLYHREDGLDIK
                     IDRCLIDANWGQSTDVVYQFCRQSRF"
     gene            complement(1764..1964)
                     /locus_tag="PLE88_12815"
     CDS             complement(1764..1964)
                     /locus_tag="PLE88_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77388.1"
                     /translation="MKNPIKITALTPQELATLLSQASRRAISGQDVLAIAEMAGIVAP
                     DGTINLIDYTAFLSQEVAGGAD"
     gene            2107..2331
                     /locus_tag="PLE88_12820"
     CDS             2107..2331
                     /locus_tag="PLE88_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77389.1"
                     /translation="MATNDVKTAYENAKTDIANLLGFFECEMSKEPDTINWGHVGSLN
                     KIRHDLIETLSFMSGFDVKEIENTLEETKL"
     gene            2347..2616
                     /locus_tag="PLE88_12825"
     CDS             2347..2616
                     /locus_tag="PLE88_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77390.1"
                     /translation="MHVIEMKLAGRMKKGDVPEYFATIHRVPGNEYITVKLISADGER
                     EHLVEANNSDDVFSMAECLQYHLDGCKGTNSMIHDYYRILENFTD"
     gene            2639..3163
                     /locus_tag="PLE88_12830"
     CDS             2639..3163
                     /locus_tag="PLE88_12830"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOM77391.1"
                     /translation="MKKSDVKIGNHYKAKVTDKVVTIEILAENPHGGWDAKNLSTGKT
                     VRIKSPLRLRGAVAKPTTSAPGGKPKQEPSKDTPKPKSPNTAKPVQNTLRTNKMSILD
                     AAAQVLSEAKEPLNCKLMVKAMTNKAYWQPSQSGKTPANTLHAAISKEIKTKAADSRF
                     EKVGRGQFALSATK"
     gene            complement(3172..>3632)
                     /locus_tag="PLE88_12835"
     CDS             complement(3172..>3632)
                     /locus_tag="PLE88_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOM77392.1"
                     /translation="YFSQGIVWDKQHPVLTRKDFMGAFEICFYGWKEGAGHKFYGPNN
                     ATDLWHVKKVNPQSMIHLTEKPVELAVRAIQYSSKPGKNVLDLFGGSGSTLIGCEQTG
                     RNAFLMEIDPPYCDVIVQRWEEFTGKKAERIVATEKTSAVDTEVQEAVTV"
BASE COUNT          930 a         1027 c          882 g          793 t
ORIGIN      
        1 aaaaccgtga ttgacgacag aactgataga ccacatcggt cgattgtccc cagttggcat
       61 caatcaggca acggtcaatc ttgatatcca ggccgtcttc tcggtgataa aggcaggcca
      121 gtttctcttc ggtcagttgt tggagggcat gataaatcgc accttccagc ccggcccccg
      181 gtttttgctg ggcaattgtg cgtcggatgt ctcgcagcgt aaaatacagc cgtttctgtt
      241 ccggccatgt gccatagtcc aggatatagc cggtaaaatc agtctcccac ccacacagca
      301 tccagaacag agctttctgc tgaacgtcaa caaacattgt caggttatga caggaaatcg
      361 gaacgacgcc acggttgtat ccattgagtt tggcgacgat ttggtccgca ttgagcatct
      421 cctcgccttc agtctcgaca accggttcat tttggtattc ggcaaagaac gcggcctcat
      481 cgcggagcct caagttcatg gcatgctgaa tagccgacaa ttcatcgatg ttgtgccgtt
      541 cgggccaggc aatcacagaa ccggcatcca ttgcctcgcg gttttcacga tagaactccg
      601 tcgcctgtga accatcccca tcattccgca acgaatcagc acggatctcg gcatattggg
      661 cccagagttt ctcattggtc gggaacacat agaccatctt cgtccgttcc ccttgccatt
      721 cggggtgttt ttcgcggtcc aggatgttat ccgccatatc accgggccgg atgacggtac
      781 aggccatcaa accggatatc ttcttaccgg gaccggccat gcccaggaca tcgcccgcta
      841 aaatagcctc acgccgctgg gattgggagg gactccaggc cgattcggtc gtctgcgggt
      901 catcaaccat caccagttgt gggcggacca ccttgccatc cgcacgagca tggttttgac
      961 cgcggatgtc cgaacccttc atccccgaac aggagattac cacgccggat gcggtgcttc
     1021 cctcgatggt aggaaggaca attttatccg cggcccattc aatgcgggtg gagacaccat
     1081 tatgtttttg acccttttga cggttggtaa tccgctccaa tgccaaaatc ggcaacgtca
     1141 cctcggggaa atcttcgtgc agacgttcat tggtctccag ccagaccttg atgttttcca
     1201 gcaggttttt ggcgcggtcc gccgaagcgg caatcagaca cacaaactct gtcgccccaa
     1261 tcagtgctga ccacaaacag gccatctgca ttaaaaccgt tttaccgctg cctcgaggca
     1321 tagccaaggc gaacagacca cctttcagaa ccgccagttc aatcttggcg ataaccttgt
     1381 gatggtcggc cgaccacgtc aggtagaata catccggaaa ataagcatcg caaaaaaact
     1441 taaaactggc catcgccttg gttttacggt ccggattgac caccgccggg atatgaccga
     1501 tgtcctgacc ggcccgtgcc agttcggcat tccgtaaccg ggccgcttct cgaatctggt
     1561 catagttacg ggattccttc ggcggcgcta aatactgctg tgtcaaccac gctgcgtatt
     1621 ggaataaatt caccgttcgg gcgtcgccga tggtgtaacc ggcacggttc cggtggcgac
     1681 ggagcgtgcg ttcattgagc acctccccga atccagcaga attcagaagc cgcgtcaggt
     1741 cggtgggttt gagtttggtc ggattaatct gcgccaccgg cgacctcctg ggataaaaac
     1801 gcggtgtaat caatcaggtt aatcgtgccg tccggtgcca cgatcccggc catttcggca
     1861 attgccagca cgtcttgtcc ggatatcgcg cgacggcttg cctggcttaa caatgtggcc
     1921 agttcctgtg gcgttaatgc ggtaattttg atgggatttt tcattgctta caacctttgt
     1981 ccggaaccta aaaaataatg caagttcttt gaaaataatg acttaattcg cttggattcc
     2041 atccccaaag ccggtagatc atgatcagaa ttgaaaaata aaaacgccaa tacaggagac
     2101 aaaaccatgg caacaaacga cgtaaaaacc gcatacgaaa acgccaaaac cgacatcgcc
     2161 aatctgctgg gattcttcga atgcgaaatg tccaaagaac cagacacaat aaattggggc
     2221 cacgttggca gcctcaacaa aatcagacat gacttgatcg aaacgctgtc cttcatgtcc
     2281 ggatttgatg tcaaggaaat cgaaaacaca ctcgaagaaa ccaaactgta agaaaggaac
     2341 agacccatgc acgttatcga aatgaaactg gccggacgta tgaaaaaagg cgacgtaccg
     2401 gaatacttcg ccaccatcca ccgcgtcccc ggcaacgagt acatcaccgt caaactcatc
     2461 agcgccgacg gcgaacggga acatttggtc gaagcgaaca actccgacga tgtattctcg
     2521 atggccgaat gcctgcaata ccacctcgac ggatgcaaag gcaccaattc gatgattcac
     2581 gactattacc gtattttgga aaattttaca gactaacatt tttttgaaag gaccaatcat
     2641 gaaaaagtca gacgttaaaa ttggaaacca ctacaaagcc aaagtcaccg acaaggtggt
     2701 aaccatcgaa atcctcgccg aaaatccgca tggcggctgg gatgccaaga acctatccac
     2761 cggcaagacg gtgcggatca agtcgccgct gcgcctgcga ggggcggtcg ccaaacccac
     2821 cacgtcggcc ccgggtggca aaccgaagca agagccgagc aaggataccc ccaaaccaaa
     2881 atctcccaac acggccaaac ctgtgcagaa cacgctgcga accaacaaga tgtccatcct
     2941 ggatgccgcc gcccaggttc tttccgaggc gaaggaaccg ctcaattgca aattgatggt
     3001 taaggcgatg acgaacaaag cctactggca accatcccaa tccggcaaaa cacccgccaa
     3061 tacgctgcac gcggccatca gcaaggaaat caagaccaag gcagccgact cccgctttga
     3121 aaaggtgggc cgagggcaat ttgccctcag cgccacaaag taacgatagc attaaaccgt
     3181 taccgcttct tgaacctcgg tatcaactgc cgaggttttt tcagtggcca cgatccgctc
     3241 ggccttcttt cccgtaaact cttcccaccg ctggacaatc acatcgcaat acggcgggtc
     3301 gatttccatc aggaatgcat tacggccggt ctgttcgcat ccgatgagcg tgctgccgga
     3361 cccgccgaac aggtccagga cattcttgcc gggtttggag gaatactgaa tggcacgtac
     3421 cgccagttcc actggctttt ccgtcaggtg aatcatgctt tgcggattga cctttttgac
     3481 atgccacagg tcggttgcat tgtttgggcc gtagaattta tgaccggcac cttccttcca
     3541 gccatagaaa cagatttcaa atgcacccat gaagtccttg cgggtcagga ccggatgctg
     3601 cttatcccac acaatccctt gtgaaaagta ca
//
LOCUS       DAOTBO010000189         3597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20756_length_3597_cov_4.500282, whole genome shotgun sequence.
ACCESSION   DAOTBO010000189 DAOTBO010000000
VERSION     DAOTBO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20756_length_3597_cov_4.500282"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLE88_12840"
     CDS             <1..247
                     /locus_tag="PLE88_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77393.1"
                     /translation="AYNEQLMKLIDEQYIKTPFYEMDKMTASLRHYPSQSDLLGGDHL
                     KNSFSVWTMGRRFNYPSGTAIFAAFNFLKICSKIKAV"
     gene            578..1105
                     /locus_tag="PLE88_12845"
     CDS             578..1105
                     /locus_tag="PLE88_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77394.1"
                     /translation="MNKDSQKHKRFFQFYNSVHTSLYSFLFVLVHNQVDAEELLQETA
                     AVLWEKFDTFQEGTSFGAWAIQIAKYKALEFLRSSKRMRMIFEDAAYMAISEEAEKHS
                     PEMGSYVRALNKCLGRLVEQDYKLLSLRYRKNLSVKQIAQMIGRSRTGLYQSYSRIFE
                     LLRVCIQKKLAQMEV"
     gene            1110..2816
                     /locus_tag="PLE88_12850"
     CDS             1110..2816
                     /locus_tag="PLE88_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM77395.1"
                     /translation="MEHLHQQIQARMLLVRLIENDIDEEGIRFLNRWFKEDPEAVRCY
                     CEFLRVYAVSQLQISNKIDCQTALEDSQFDQAIWTALKEMEDAAPVVEVTVPLPVPRR
                     SIEVPKTEKRISKKALAAAIISGAALLFMICYVHFFSPAEMSRPVASLTGSYNTLWSG
                     TDESMPNGSRLWNDGRWYRLERGKAEITFDSGAAVLLEAPVRLEVLSENEMLFEGTLT
                     ARVPVSAHGFTVYTAHSTIVDLGTEFGVFADAQESRVFVRQGEVELEALFAPHSSLKQ
                     RISSGKGYQVDRSGTISEVVYHPDTFRWESPDLYEQAVYATNPIAYWRFDRDLQERLI
                     NEMNPKDVSGRCAGVVQRRAAGPNLGSGKPNFALRLLGRKAGEQEAIPAGLARIGSET
                     TDWKRTGNYSIVLWFCPETPGLQSILVYSNEDTYGILSRYSDQIYLSKDNRISFYVYC
                     PESAADNQALHSISAAEPVQLNTWYHVAVTCSAENRINLYVNGRLRAAKQLPSRPQPN
                     LIGYIGCSTINPYYDQVEHLALSREPFCGVVDEISEYDRTLSAEEVENLYRAAGGTEE
                     RK"
     gene            2821..>3597
                     /locus_tag="PLE88_12855"
     CDS             2821..>3597
                     /locus_tag="PLE88_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOM77396.1"
                     /translation="MMIPSSTILTDRIVKRLKGFTLIELLVVISIIGLLLSVLIPALS
                     KAKRFAEEIVCKNNLHQYQLVTEMYAQENKELLPIPQESIFASDSVLQAAGEVQKYCR
                     WHNPLFDLDEHPEFGGPLQPYLQVAQVSVCPVFRKLSAKYASFHPNHNTAVATGVITF
                     SLTMNGNLQRMMQPDTYVFKSIRKTEIRSPSQVFLWAEENMWTLNDMNGNPLSSAVLN
                     DTTLLAGSDCFASFHQLSAGKIEVQIGTGTYNGGSSNVLMC"
BASE COUNT          902 a          858 c          965 g          872 t
ORIGIN      
        1 agcgtataat gagcagttga tgaaacttat tgacgagcaa tatatcaaga cgccgtttta
       61 cgagatggat aaaatgacgg cgtcgctgcg tcattatccg tcccaatcag acctgctcgg
      121 cggagatcac cttaaaaact ctttttctgt ctggacaatg gggaggaggt tcaattatcc
      181 ctccggaacc gccatctttg cagccttcaa ctttctgaaa atttgctcaa aaattaaagc
      241 agtatgaaac gatataacct gttgccgaaa atagacttat ataaaatttc tatttaaaat
      301 ccatttttta cctgacaaat caaaggtgtg catcatttta ttgtgaggaa tgcaaatata
      361 ggaaaaggtc aaattgactg acttttgagg atgggatttt ggacgatgat gaccatcttt
      421 tgcagcagaa aacggtattc tcttccataa aagggctgaa aagcactctt ttgtacagga
      481 gactgccgtt aggtttcagg tcgtctgttt cccgcaaaag ccaaggtgaa taagaacgaa
      541 acatgatatt acaaaatggt ttacgaagtt ggcggtgatg aataaagatt ctcagaaaca
      601 taagcgtttt tttcagtttt ataacagtgt acatacgtca ctttactcat ttctgtttgt
      661 cctggtccat aatcaagtgg atgctgaaga attgctccag gagacggcag cagtcctctg
      721 ggagaaattt gatacgtttc aggagggaac cagctttggt gcatgggcaa ttcagatcgc
      781 caaatacaag gccctggagt tcctgaggag cagcaaacgg atgcggatga tttttgaaga
      841 cgccgcttat atggccattt ccgaggaggc tgaaaaacat tccccggaaa tgggaagcta
      901 tgtgcgggca ctgaataaat gtttaggtcg cctggttgag caggactaca agctgttgtc
      961 gctgcgttac cggaagaact tatccgtcaa gcagattgcc cagatgatag gccgctcccg
     1021 aaccggatta tatcagagtt actcccgcat cttcgaactg ctgcgagtct gcattcaaaa
     1081 aaagttagct caaatggaag tctgatccga tggaacattt acatcagcaa atccaggctc
     1141 gaatgctgct ggttcggctg atagaaaacg atatcgatga agaggggatc cgttttttga
     1201 accggtggtt caaagaagat ccggaagcgg ttcgctgcta ttgtgaattt ctccgggtgt
     1261 atgctgtgtc gcagttgcaa atctccaaca aaattgattg tcaaaccgct ttagaggaca
     1321 gtcaattcga ccaagcgata tggacagccc tcaaggaaat ggaagatgcc gctccggttg
     1381 tcgaggtcac ggtgccgttg ccggtacccc gacgttcgat cgaagttccc aaaacggaga
     1441 agcgcatcag caagaaggca ctggcggcag cgatcatttc aggggcggct ttattgttta
     1501 tgatttgtta tgtccatttc ttctcccccg ctgagatgtc gcgccctgtt gcgtcgctga
     1561 ccggcagcta taataccctt tggtccggga ctgatgagtc gatgccgaac ggcagtcgat
     1621 tgtggaacga cggccgctgg taccgcctgg aacgcgggaa agcggaaatc acgtttgaca
     1681 gcggggccgc cgttctgctt gaagccccgg ttcgtctgga ggtcctcagc gaaaacgaga
     1741 tgcttttcga ggggacgctg acggctcgag tgcctgtgtc agcacacggc tttacggtgt
     1801 atacagcgca ttcgaccatc gtggaccttg ggacggagtt cggagttttt gccgatgcgc
     1861 aggaaagccg ggtgttcgtc cgtcagggtg aagtggaact ggaggcgctt tttgcccccc
     1921 attcatcgct caagcagcga atcagctctg gaaaagggta ccaggtagat cgctcaggaa
     1981 caatttccga ggtagtgtat caccccgata ccttccgatg ggagtctccg gatttgtatg
     2041 agcaggctgt ctatgccacg aatccgattg cttactggcg gtttgacagg gatttgcagg
     2101 aacgtctgat caatgagatg aacccgaagg atgtctccgg ccgctgcgca ggtgtggttc
     2161 agcgaagagc cgcagggccg aatctgggca gcggtaaacc caattttgct cttcggctgc
     2221 tcggcaggaa agcgggcgag caggaggcga tcccagccgg acttgcgcgg atcggcagcg
     2281 agacaacgga ctggaaacga actggaaact attcaattgt tctctggttc tgcccggaaa
     2341 ccccggggct ccagagcatc ctcgtctatt caaatgagga cacctatggg attctcagcc
     2401 gctattccga tcaaatttat ctaagtaaag acaaccggat ttccttctat gtctactgcc
     2461 ctgagtcggc ggctgacaat caggcactgc attctatcag tgcggcggag ccggtccaat
     2521 taaatacgtg gtaccatgtg gctgtgacct gttccgcaga gaaccgcatc aacctgtatg
     2581 tcaacggtcg gctccgggcg gccaagcagc tcccttcccg gccgcaaccc aatctgatcg
     2641 gctatatcgg ttgttccacc atcaatccct actatgacca agtggaacat cttgccctga
     2701 gccgggaacc attttgcggc gtagtggacg agattagtga atatgatcgg accctgtccg
     2761 ccgaggaggt tgaaaacctg tacagggcag cagggggaac ggaagaaagg aagtgaatcc
     2821 atgatgatac cgagctcgac cattctgacg gatcgaatcg tgaaacgact gaagggtttt
     2881 acgctgattg agctgctggt tgtgatttcc atcattgggc tgctcctttc ggttcttatt
     2941 ccggcccttt ccaaggccaa gcggtttgca gaggagattg tgtgtaaaaa caatttgcac
     3001 cagtatcagc tggtgacgga gatgtatgct caggaaaaca aggaactgct gccgattccg
     3061 caggagtcca tctttgccag tgacagcgtg ctgcaggcgg ctggcgaggt ccagaagtac
     3121 tgccggtggc acaatccgct gtttgatctg gacgagcacc ctgaatttgg cggtcccctc
     3181 cagccctatc tccaggtggc acaagtgagt gtatgcccgg ttttccgcaa actgtctgcg
     3241 aagtacgcct ctttccatcc gaatcataac acagccgtgg cgacgggagt tattaccttc
     3301 agcctgacga tgaacggcaa cctgcagcga atgatgcagc cggatactta tgtcttcaag
     3361 tcgatacgaa aaacggaaat tcgaagccct tcccaggtct tcctgtgggc ggaagagaac
     3421 atgtggaccc tgaacgacat gaacggcaat cccctcagca gtgctgttct gaacgataca
     3481 accctgttgg caggttcgga ctgtttcgcc agctttcacc aattgtccgc cggaaaaata
     3541 gaggtacaaa tcggcacagg gacctacaac ggcggcagct ctaacgtgct gatgtgt
//
LOCUS       DAOTBO010000190         3595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20768_length_3595_cov_4.746328, whole genome shotgun sequence.
ACCESSION   DAOTBO010000190 DAOTBO010000000
VERSION     DAOTBO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20768_length_3595_cov_4.746328"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLE88_12860"
     CDS             <1..444
                     /locus_tag="PLE88_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOM77397.1"
                     /translation="SLGWRVFEGGRIRNMVKADEARMRQLLAAYEQAVLTAVGEVETA
                     AAAYVQESQREQALQRSVQAAEDTVRLVSTLYRSGLTDFQNVLDAQRTLFSQQDLLAV
                     SRGARVLNLIRIYKALGGGWDPDAAGGFPAAAAAEALQSTETIPQ"
     gene            466..1674
                     /locus_tag="PLE88_12865"
     CDS             466..1674
                     /locus_tag="PLE88_12865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOM77398.1"
                     /translation="MKGKQIIIVLIVVAVAAGIFLMRRKSSSQPAPAAQAAGPLPMPA
                     ARCVMRDVTDYYEFTGTTEAVDAVDIRARVEGYLKGIHFTDGQIVEEGQLLFTIEPEA
                     FAARRDEAAAQLKAAQAESERAWVDLQRIQNAIASNAVSQQDLTRSRAVYETAAAAVL
                     AAQARLADAQLNLSYTEIRSPIRGRIGRHMADIGNLVGPASRPVLTTVMRMQPMYVSF
                     YMGEHLLKGGLLQRLQGGGQPLAMLVGLPDEKGYPHEGTIGFLDNRVDAKTGTVYVRG
                     QLPNQDETLLPGMFVRIRLPIAQRPNAVLIPEKAILTDLGGKYVLAVGQGNVLERRSI
                     TLGASVDGMRVVTEGLDGSEMFIVGSFHMARPGMPVTPLTGDGPPAGMPAGQAAPSAS
                     NLPADQQEKQ"
     gene            1708..>3595
                     /locus_tag="PLE88_12870"
     CDS             1708..>3595
                     /locus_tag="PLE88_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOM77399.1"
                     /translation="MFSKFFIDRPIFASVISIVIVLVGLICLPLLPVEKTPDITPPTV
                     MVRATYPGANAEVLAETVATPLEEAINGVDRMLYMTSSSSDNGMVMITVMFEVGMDPD
                     MATVLVQNRVALAEPSLPEEVKRNGIVIQKRSSNISMLVAFNSPDDRYDDIYLSNYIN
                     LNIKDVLSRVPGVGEVEVMGAKNYGMRIWLDPAYLRARQLTADDVIAAIRQQNIEVAA
                     GQIGGMPSPEDQQFQFTVKTLGRLSTVEQFEDIIVRTDADGRLLRLKDVARVELGAQE
                     YVWAVKLNGQKAVAVAVYATPGANALQVVKGVRQALAELAPSFPEGLRYTEPYNPTQF
                     IEESLWEVFWTLIMVVVLVILTVFVFLEDWRATLIPSVTIPVSLIGTLAVMKAAGLSI
                     NTLSLFGLVLAIGIVVDDAIVVVENCVRLITDEKLPAKAAAVKAMQQVTGPVIATTLV
                     LLAVFAPTVFSGGITGRLYQQFTITLSIAVCFSTLNALTLSPALCAILLRPVGHKQGT
                     LFGWFDRFIRNTTSRYAAVVKMLIRRTAIVLVLFVVITVLSVVGFGKLPTGFLPDEDE
                     GTILIGVRLPEGSSLPRTMEVMDTIGGILSRTEGVDQYGAISGFSMLDSAIASNGGTC
                     FVRLK"
BASE COUNT          725 a          954 c         1111 g          805 t
ORIGIN      
        1 tcgctgggat ggcgggtttt tgagggcggg cgcattcgca atatggtcaa ggccgatgaa
       61 gcccgaatgc ggcagctgct ggctgcttat gagcaggcgg tgctgacggc ggtgggagag
      121 gtcgaaacgg ccgcggcagc ttatgtgcag gaatctcagc gggagcaggc actgcagcgc
      181 tcggtacagg ccgccgaaga taccgtgcgg ctggtcagca ctctgtaccg cagcggtctg
      241 accgatttcc agaatgtcct cgatgcgcag agaaccctct tcagccagca ggatttgctg
      301 gctgtcagcc ggggagcacg agttctgaac ttgattcgga tatacaaggc attgggcggc
      361 ggatgggatc cggatgccgc cggcggtttt ccagcagccg ctgcggcaga agccctgcag
      421 tcaactgaaa caatacccca ataaatacga aaggaaggat tccctatgaa gggaaagcag
      481 attattattg ttttgattgt cgttgcggtt gccgcaggaa tctttttgat gcgcaggaaa
      541 tcatcctccc agcccgcccc agccgcacag gcagccggac cgctgccgat gccggctgcc
      601 cgatgcgtga tgcgggatgt tacagactac tacgaattta ccggcacaac cgaagcggtg
      661 gatgcggtgg acatccgcgc ccgtgtggaa gggtatctca aaggcatcca ttttaccgat
      721 ggacaaatag tggaagaagg gcaattgctt tttacgattg agccggaagc gtttgccgcg
      781 cggcgcgatg aagcggctgc gcagcttaaa gcggctcaag ccgagtccga gcgggcctgg
      841 gtggatttgc agcgcattca aaacgccatc gcctctaatg ccgtcagcca gcaggacctg
      901 acccgcagcc gggctgttta tgagacagcg gcggcggctg tattggccgc tcaggccagg
      961 ctggccgatg cccagctgaa tttgagttat accgaaattc gcagcccaat tcggggccgc
     1021 attggacgtc atatggccga tatcggaaat ctggtaggac cggcatcgcg tccggtgctg
     1081 acaacggtga tgcggatgca gccgatgtat gtgtcgtttt atatgggcga gcatctgctc
     1141 aaaggcggcc tgctgcagcg tctgcagggc ggcggccagc cgcttgcgat gctggttgga
     1201 ctgcccgatg aaaagggcta tccccatgaa ggaacgattg gttttctgga caatcgcgtc
     1261 gatgccaaaa ccggtacggt ttatgtccgc ggccaattgc cgaatcagga tgaaacgctt
     1321 ctgccgggta tgtttgtgcg gatccggctg ccgatagctc agcgtcccaa tgccgtgctg
     1381 attccggaaa aggccatcct gaccgatttg ggcggcaaat atgtcctggc tgtcggacaa
     1441 ggcaatgttc tggagcggcg cagcattacg ctgggggcct ctgtggacgg gatgcgcgtt
     1501 gttacagaag gcctcgacgg cagcgagatg tttattgtcg gcagctttca tatggcccgg
     1561 ccggggatgc ccgttacgcc tttaaccggc gatggtcctc cagccggaat gccggcagga
     1621 caggcggctc catcggcctc caatctacct gccgatcagc aggaaaaaca ataatcaagc
     1681 gaaccgattg gtgttccagg agattgtatg ttcagcaagt tttttataga caggcctatc
     1741 tttgcctccg ttatttcaat tgtgattgta ctggttgggc tgatttgtct tccgctgctg
     1801 ccggtggaaa aaacgcccga tattacgccg ccgaccgtga tggtgcgcgc cacataccct
     1861 ggtgccaatg ccgaggtgct tgccgaaacg gtggcaaccc ccttggaaga ggccatcaac
     1921 ggcgtcgatc gcatgctcta tatgacgtcc agcagcagcg acaacgggat ggtgatgatc
     1981 accgtcatgt ttgaagtggg aatggatccg gatatggcca ccgtgctggt gcagaaccgc
     2041 gtggcactgg ccgaaccctc tttgccggaa gaagtcaagc gcaacggcat tgtcattcag
     2101 aagcgctcca gcaatatctc gatgctggtg gcctttaact cgcctgatga ccgctatgat
     2161 gatatctact tgagcaacta catcaatctg aacatcaagg atgttctgtc gcgggtgccg
     2221 ggtgtgggcg aggtggaagt aatgggagcc aaaaactacg gtatgcgcat ctggctggat
     2281 ccggcctacc tgcgggcacg ccagctgact gccgacgatg tcattgcagc gattcgccag
     2341 cagaatattg aagtggccgc cggtcaaatc ggcggcatgc cgagccccga agaccagcag
     2401 tttcagttta ccgtcaaaac cctcggacgc ttgagcaccg tcgagcagtt tgaggacatc
     2461 attgtgcgaa ccgacgccga cggccgcctg ctgcggctta aggatgtagc ccgcgtggag
     2521 ctgggggcgc aggagtatgt ctgggctgtg aagctcaacg gccagaaggc cgtggcagtt
     2581 gccgtctatg caacccccgg cgccaatgcc ctgcaggttg ttaagggcgt ccggcaggcc
     2641 cttgccgaat tggcaccgtc ttttccagaa ggcttgcgct ataccgaacc ctataatccc
     2701 acccagttta ttgaggaatc gctgtgggaa gtgttctgga cgctgattat ggtggttgtg
     2761 ctggtgatcc tgacggtatt tgtgtttttg gaagactggc gagcgaccct gattccttcg
     2821 gtaacgattc cggtttcgct catcggcacg ctggccgtga tgaaagccgc gggattgtcc
     2881 atcaataccc tctcgctgtt cgggctggtg ctggctatcg gcattgtggt ggatgatgcg
     2941 attgtggtag tggaaaactg cgttcgtctt atcaccgatg aaaagctgcc tgccaaggcc
     3001 gctgccgtca aggccatgca gcaggtgaca ggaccggtta tcgcaaccac gctggtgctg
     3061 ctggcggttt ttgcccccac ggtattcagc ggcggaatta ccggccggct ctaccagcag
     3121 tttaccatta cgctgtcgat tgcggtctgt ttttccacgc ttaacgccct gacgctcagt
     3181 ccagcgctgt gtgctatcct cctgcgtccg gttggacaca agcaggggac tttgtttggc
     3241 tggtttgacc ggtttatccg caatacgacc tcacgctatg cagcggttgt caagatgctt
     3301 atccggcgta cggcgatcgt attggtttta tttgttgtga ttacggtttt atcggttgtc
     3361 ggattcggca agctgccgac aggctttctg ccggatgagg atgaaggcac cattttaatc
     3421 ggcgtccggc tgccggaggg ctcttcgctg ccccggacga tggaagtgat ggataccatc
     3481 ggcggcattc tcagccgaac ggaaggggtg gaccaatacg gcgccatcag cggcttttcc
     3541 atgctggaca gtgcgatagc ctccaacggc gggacctgct ttgtccggct caagc
//
LOCUS       DAOTBO010000191         3585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_20848_length_3585_cov_3.742493, whole genome shotgun sequence.
ACCESSION   DAOTBO010000191 DAOTBO010000000
VERSION     DAOTBO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20848_length_3585_cov_3.742493"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLE88_12875"
     CDS             <1..677
                     /locus_tag="PLE88_12875"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77400.1"
                     /translation="TVAVWTDLAGGDHNATSAGTPTYVANALNGLPVIKIDGTSDRYG
                     NGLPNSYDFYAIPTVSDVKTIAMVFKPTSNSYYTWAPIMAGPTGDNYGWHGDTHWGNA
                     YWDYAYSVSSIANSNLAINGIGGYNGGYTGVDYDAFHVITMQLAAGTPFDLGFLAHTA
                     RYNDYNTYGMEIAELMVYTQQLSTAQLEQLTAHLGNKYGIAVAVPEPGTLVLLGLGAC
                     LLKRGK"
     gene            827..2179
                     /locus_tag="PLE88_12880"
     CDS             827..2179
                     /locus_tag="PLE88_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOM77401.1"
                     /translation="MSCPKSEAVQKKAPKRLDIEVVARSDKTVRHCTSRQRLIDTLNH
                     RQPDRLCVDFGAGGQTGMGAGAVYRLRRALIGQSDYRVKIIEPFQMLGEIDQQLRNVL
                     RLDVAGFNPPTNMFGFRDEGWKPFDMPDGTPVLVPEKFNYTVDADGGILMYPQGDTSV
                     APCAKMPAAGYFFDALNRQQPFREEDLDPADNCQEFGLLSDEDVQYFADTASNLYDHT
                     EYGIYMTLPGTAFGDIALVPATFMKNPRGIRDVEEWYISTALRRDYIYKVFETQCAFA
                     VQNIQRLAHAVGDKVQVVFVSGTDFGTQRGLFISKQTYRELFKPFHKAVNDTIHRLTS
                     WKTFIHSCGAVFELIPDFIEAGFDILNPVQCSAEGMDPKVLKNEFGKQIVFWGGGVDT
                     QKTLPFGTPDEVYRQVRERIDIFAEGGGFVFNSIHNVQSNVPTENLLAMFRAIDDARA
                     "
     gene            2280..>3585
                     /locus_tag="PLE88_12885"
     CDS             2280..>3585
                     /locus_tag="PLE88_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOM77402.1"
                     /translation="MELQVMDLVVFAGFFAVVVGTSVFKSRREKSSEDYFLAGRGLTW
                     WLIGISIVAANISTEQFVGMAGQGAGSVGLAVSNWQLIGSVGIVLIAFTLLPRFLQAG
                     IYTMPEYLEYRYNSLARAIMAVITVIIYVAVLLTAVLYSGGLTLKTIFGFSLVRSIWL
                     IGLIAAAYTAWGGLKAVAWADLFQGLALLGGGIVVFILGLRACGGWDHFASLNSEKLH
                     MILPASNAGLPWTGVAGGMWIVLVYYCGLNQFIVQRNLAAKSLRDGQLGVIFAGALWL
                     LVPFAIVMPGIMSYQLYHPQMQSPDEAFPLLIRNLIPQGLRGFMFAAIAGAVISSLAS
                     MLNSAATIFTMDVYHRMLKPGASQKELMVLGRAMTLLFVLAGCLLAPLLANPKFGGVF
                     QFIQQFQGYIWPGVVAAFLFGMLVPTAPGAAGVAALVSGPIIY"
BASE COUNT          781 a          851 c         1020 g          933 t
ORIGIN      
        1 ataccgtggc agtctggacg gatttggccg gcggcgatca caacgcgaca tcggctggta
       61 cccccacgta tgtcgccaat gccctcaacg gtctgccggt tattaaaatt gacggcacca
      121 gcgaccgtta cggcaatggt cttccgaaca gctatgattt ttacgccatc ccgactgttt
      181 cagacgtaaa aaccattgca atggttttca agcccaccag caacagctat tacacatggg
      241 ccccgataat ggccggacct accggagaca attacggctg gcacggtgat acccattggg
      301 gaaacgctta ttgggattat gcgtattccg tcagctcaat tgcaaattcc aatctggcaa
      361 tcaatggcat cggcggctat aacggcggct atacgggcgt ggattatgat gctttccatg
      421 tgattacgat gcagcttgcc gccggaacac cgtttgatct gggctttttg gcccataccg
      481 cacggtacaa cgactataac acctatggta tggaaatcgc tgaattgatg gtttataccc
      541 agcagctaag cacagctcag ttggagcagt tgactgcgca tctgggtaat aaatacggta
      601 tcgccgtcgc tgttcccgag ccgggaacac tggttttgct gggattggga gcatgtttac
      661 tcaagagggg aaaataggcc cgggtttaca attgttctgt gcagggatta gcccctgaca
      721 gcaataacgc caaataggaa gctccatcaa gcaggatcga cggagctttc tatttggcca
      781 agatgagagt gttggcatca atggatacaa gcaaaagaga ttcagcatga gttgtccgaa
      841 atccgaagcg gtacaaaaga aagcccccaa gcggcttgat attgaagtgg ttgcaaggag
      901 cgataaaacc gtgcggcatt gcacatcacg gcagcggctc atagatacgc tcaatcaccg
      961 gcagccggac cgtttgtgcg tggatttcgg tgcaggcggc cagacaggaa tgggggccgg
     1021 tgccgtgtat cggctgcgtc gggcgctgat aggacaatcc gattatcggg tcaagattat
     1081 tgagccgttt cagatgctcg gcgaaataga ccagcagctg cgcaatgttc ttcgcttgga
     1141 tgtggcagga ttcaacccgc cgaccaatat gtttggtttt cgagacgaag gctggaagcc
     1201 gtttgatatg cccgacggca cacccgttct ggtgcctgaa aaatttaatt atacagtcga
     1261 tgccgacggg ggaattttga tgtacccgca gggggatacc tccgtggccc cctgtgccaa
     1321 gatgccggct gccggctatt tttttgatgc cctcaaccgc cagcagcctt ttcgggaaga
     1381 agaccttgat ccggccgata actgccagga gtttggtctg ctcagtgatg aggatgttca
     1441 gtatttcgcc gacacagcat ccaacctgta tgaccatacc gagtatggga tttatatgac
     1501 cttgccgggg accgcattcg gcgacatcgc attggttccg gccacgttta tgaaaaatcc
     1561 ccgcggcatc cgcgatgtgg aggagtggta catttcaacc gcactgcggc gggattatat
     1621 ttataaggta tttgagaccc agtgtgcatt tgcagttcag aatatccagc ggctggccca
     1681 tgcggtcggc gacaaagttc aggtcgtatt tgtcagcggg accgattttg gcacccagcg
     1741 cggattgttt atctccaagc aaacctaccg ggagctgttt aagccgttcc ataaggccgt
     1801 caatgacacg attcaccgat tgaccagctg gaaaacattc attcattcct gcggcgctgt
     1861 atttgaactg attccggatt ttatcgaagc gggctttgac atactcaatc cggttcagtg
     1921 ctcggccgag gggatggatc ctaaggttct taagaacgaa ttcggcaaac agattgtttt
     1981 ttggggaggc ggtgtggata cccagaaaac ccttcccttc ggaacccccg atgaagtgta
     2041 tcggcaagtc agagaacgga ttgatatttt tgcagagggc ggcgggtttg tatttaacag
     2101 cattcataat gtgcaaagca atgttcccac cgaaaacctg ctggcgatgt tcagggcaat
     2161 tgatgatgcc cgtgcataac tgattcaatc aatggcatgc agaatggtac cgaatcactg
     2221 gcttgctgtt gtctttcctg ttataatccg gccaaaaaga gtcagcgaaa ggagtcttta
     2281 tggaactgca ggtaatggac cttgttgtat ttgcaggctt ttttgcggtt gttgtaggaa
     2341 catcggtttt taagagccgc agagagaaaa gcagcgaaga ttattttctg gccggacgcg
     2401 gattgacgtg gtggctgata ggcatatcga ttgttgcggc caatatttca accgagcagt
     2461 ttgtgggtat ggcaggccag ggggccggca gtgtaggact ggccgtcagc aattggcagc
     2521 ttatcggcag tgtcggcatt gtgctgattg cttttacgct cctgccgcgc tttctccaag
     2581 ccggcattta tacgatgccg gaatatctgg agtaccgcta taattccctg gcccgcgcca
     2641 ttatggcagt catcaccgtg attatctatg tagccgttct gctgacggcc gtcttgtatt
     2701 ccggcggctt gacgctcaaa accattttcg gtttttcgct tgtccgaagc atctggctga
     2761 tcggtctgat tgcggctgcc tatacggctt ggggagggtt gaaagcggtg gcgtgggccg
     2821 atttgtttca ggggctggct ttgctgggcg ggggaattgt tgtgttcatt ctgggattga
     2881 gagcctgcgg cggatgggat cattttgcca gtctcaactc agagaagctt catatgattc
     2941 tgccggcctc gaatgcaggt ctgccgtgga cgggggtggc cggcgggatg tggattgtgc
     3001 tggtgtatta ctgcggtctg aaccagttta tcgtgcagcg gaatctggcg gcaaaatcgc
     3061 tgcgcgatgg gcagctgggt gttatttttg ccggtgcctt atggctgctg gttccatttg
     3121 ccattgtaat gcccgggatt atgtcctatc agctttacca tcctcagatg cagtctccgg
     3181 atgaggcatt tcctcttctg atacgcaatc tgattccgca gggattgcgg ggctttatgt
     3241 ttgctgctat tgccggtgcg gttatcagtt cgctggcctc catgctcaat tctgcggcaa
     3301 caatttttac aatggatgtc tatcatcgca tgctgaaacc cggtgcctcc caaaaagagc
     3361 tgatggtgct tggtcgggca atgactcttt tatttgtatt ggccggatgc ctgttggcac
     3421 cgctgctggc caatccgaag ttcggaggcg tatttcagtt tatccagcag tttcaaggtt
     3481 acatctggcc gggagtagtg gccgcctttt tgttcggaat gctggttccg acagcgcccg
     3541 gggcggccgg agtggccgcg ctggtttcag ggccgattat ctatg
//
LOCUS       DAOTBO010000192         3555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_21057_length_3555_cov_3.778000, whole genome shotgun sequence.
ACCESSION   DAOTBO010000192 DAOTBO010000000
VERSION     DAOTBO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21057_length_3555_cov_3.778000"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..2880
                     /locus_tag="PLE88_12890"
     CDS             133..2880
                     /locus_tag="PLE88_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM77403.1"
                     /translation="MIKNNVLILLLGVCTNTLFSAVPVHEWLFNEGNGVTVADTGTGA
                     KDGTLMDGTTSAVHGPRWVIDPVRGCVLEFDGTDWILTNSQGILGSNPRTVTAWIYLL
                     ADLNRHTIIQWGNGNGNCSGQYFRLLIENRRLRFEVASGNALALNEGALELNRWYHIA
                     LAVDDFNKDGAVRTPEVKFYLNGQNVAQTQSTSQIINTTFTDSNCYVRLGGAAQCSGS
                     SIPREAMKGRLDDIRIYNYALSASEIQQEMMSSEIAWAPIPANNSMVPVSTASLSWQP
                     GKYAQTHQVYFGTNQTDVSTATPADPRGVYLASAQINGPNTNGRYEIVLADEGLSLVG
                     NTSYYWRVDEVNSSNPASPWKGNIWKFTTNTLDTVPKNLVYTGDLSLLIFKWEPPLDI
                     QQPRYNVLLASDPNFQQIVSRATGLDSALWQADTSAMVYEKQYYCRVEAYEANDPAVR
                     GFSDRIALIFNAPRNIENFDQYQQDSELLAAWQDGSSNSTGSRIILCYEANGQSMKLA
                     YKNNISPYFSEVQYTYPSVQNWLSGGTRILQLSFRGMPANSPAALYVKLADEQNHSAK
                     VYYPGGSGYIVQPQWSPWTLWRINLNEFTSQEVNISRIRSFLIGVENSPAQPGNGEIY
                     IDSICLDIPRCPASQTIPADLNRDCISDMKDLILLAGNWMATSYWASAASSEPDGLLA
                     SYSFDETEGTVVHDNSDNLFHADLISPNPTAAWDAGGIHNGCINLTGASSVLLPAGVF
                     NSIDSAFTISFWIYFETEDQRQDSCIEFIISNGSSYSTSADRVIYSYRELSAGWHHLC
                     VTKDKASPIARIYLDGVIAGENQSALTDMSGSSIGISRLGADWTANFGFSHGKIDEFR
                     LYSRALSQSEIVYLYEGPSGHVLQPLVPIISSFDPVQDNKIDLNDFLQIASCWLLENL
                     Q"
     gene            3088..>3555
                     /locus_tag="PLE88_12895"
     CDS             3088..>3555
                     /locus_tag="PLE88_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOM77404.1"
                     /translation="MNRREFIKTLSFTAGATAFSSRVCADTLFSNQSIQVRQGKRPNI
                     ILILADDMGFADIGCFGSEIETPNLDKLAAEGIRFTQFCNSARCCPTRASLLTGLHPH
                     QAGIGWMTDDWGASKRAALNSPAYTDGLNKNCVTIAEALKRGRYHTIMSGKWHV"
BASE COUNT          961 a          799 c          852 g          943 t
ORIGIN      
        1 atcagaaatt ggtttcaagg gcacctctaa aaatttcgtt tttagaggtg cccttatgtc
       61 tcgtccctgc atggggcaag aggcaatgga ttgctgccga tatatatatc agcagcagag
      121 gtattgaatg ctatgattaa aaataatgtg ttgattcttc ttttgggggt gtgcacaaat
      181 accctgttct ctgcggtgcc tgtccacgaa tggctgttta acgaaggaaa tggtgttacg
      241 gttgcagaca caggaacagg agccaaagat ggaacactga tggatggcac gaccagtgcc
      301 gttcatggcc cccgctgggt tatcgatccc gttcgcggat gcgttcttga atttgacggc
      361 acagactgga tactgaccaa ttcacaaggt attctgggtt cgaatccaag aaccgtaacc
      421 gcctggatat acctgctggc agatttaaat cgccatacca ttatccaatg gggaaacgga
      481 aatggaaact gcagcgggca atatttccgg ctgcttattg aaaaccgacg tttgcgcttt
      541 gaagtggcca gcgggaatgc cctggcctta aacgaggggg ctctggagtt aaatagatgg
      601 tatcatattg cgctggcagt cgacgatttc aataaagatg gagccgtccg aacgcccgaa
      661 gtaaaatttt atctgaatgg gcagaacgtt gctcaaaccc aatcgaccag ccaaatcatt
      721 aacaccacgt ttaccgacag taattgttat gttcgacttg gcggggccgc tcaatgcagc
      781 ggatcatcca ttccgcgtga agcaatgaag gggcgattag atgatattcg aatctataat
      841 tatgccctgt ccgccagcga aatacagcaa gaaatgatga gttctgaaat cgcctgggcg
      901 ccgattcctg ccaataattc gatggttcct gtatcgaccg cctcattatc ctggcagccg
      961 gggaaatacg cccagactca tcaagtttat tttggaacca atcagacgga tgtcagtacg
     1021 gccacgccgg cggaccctcg aggagtttat ttggcctcgg ctcaaatcaa cgggccgaat
     1081 acaaatggcc gatatgaaat cgtcctggcg gatgagggac tttctttggt cggcaacaca
     1141 tcctattact ggagagtcga tgaggtaaat tcatccaatc ccgccagccc atggaaaggc
     1201 aatatctgga agtttacaac aaacactctg gataccgtac ctaaaaatct ggtttacacc
     1261 ggagacttat cactgctgat atttaaatgg gaaccgccgc ttgacattca gcagcctcgc
     1321 tataatgttc ttttggcctc cgatccgaat tttcagcaaa ttgtatctcg ggcgacaggg
     1381 ctggatagtg ctttatggca ggcggatacc agtgcaatgg tttatgaaaa acaatattat
     1441 tgccgtgttg aagcttatga agcaaacgat ccggctgtca gaggttttag tgatagaata
     1501 gccctcatct ttaatgcgcc ccgtaacatt gaaaactttg accagtatca gcaggacagt
     1561 gaacttttgg cggcgtggca ggacggctcg agcaattcaa caggttcgag aataattctt
     1621 tgttatgagg ccaacggcca atcgatgaaa ctggcttata aaaacaatat ctcgccctat
     1681 ttctccgaag tacaatatac ttatccatcc gtccaaaact ggctttcggg ggggacacga
     1741 attctgcagc tgtcattcag aggtatgccc gcaaatagtc cggcagcgct gtatgtaaaa
     1801 cttgcggacg aacaaaacca ttctgcaaaa gtttattatc cgggcggcag cggttatatt
     1861 gtccagccgc agtggtcacc ctggactcta tggcgcataa acttaaacga gtttacatcc
     1921 caggaagtga atatcagccg tatccggtcc tttcttatcg gtgtggaaaa cagtcctgct
     1981 caaccaggca atggagagat atacattgat tcaatttgtc tggatatccc ccgctgccct
     2041 gcgagtcaaa ctataccggc ggacttgaat cgggactgca tatccgatat gaaagattta
     2101 atactgcttg ccggcaactg gatggcgacc tcctattggg cttccgccgc gtcaagtgaa
     2161 ccagacgggt tactcgcttc ctactccttt gatgaaacag agggaactgt tgttcatgac
     2221 aattcggaca atctgttcca tgccgacctt ataagcccaa acccaacggc ggcatgggat
     2281 gccggaggaa tacacaatgg atgcattaac ctaaccggcg catccagtgt actccttccg
     2341 gcgggtgttt ttaattcgat cgattccgct tttacaatca gtttttggat ttattttgaa
     2401 acagaagatc agcggcagga cagctgcatt gaatttatca tcagcaatgg ttcttcctat
     2461 tcgacttcgg cggatagggt gatttactcc tatcgggaat tatccgccgg ctggcatcat
     2521 ttatgtgtga caaaagataa agcatctcct attgctcgta tttatctgga tggagtaatt
     2581 gcaggtgaaa accaatcggc cctcaccgat atgagcggtt catcgatcgg aatctcccgt
     2641 cttggtgctg attggacggc caattttgga ttttcgcatg gaaaaattga cgaatttcgg
     2701 ctgtattcaa gagcgctatc ccaaagtgaa atagtctatt tgtatgaggg accctccggg
     2761 catgttttgc agccgctcgt tcccatcatt agttcttttg atcccgtaca ggataataag
     2821 attgatctga atgattttct gcagattgct tcgtgttggc ttctggaaaa tcttcaataa
     2881 aagccgatct gaatagttgt actttgaggg acagagaaag tgagcacata atggagcgac
     2941 tgaataagca atccagaaaa ccatctcgct ttttgatgcc caacgaagta ttttataaac
     3001 gtaaaaaatt aaccggataa caaaacagga ttttccttaa ttgcactttg gatctgaatc
     3061 tgtctgataa gaaatggagt agagtttatg aataggcgag aatttattaa aacattgagt
     3121 tttaccgccg gagcaacagc gttttcctcc cgtgtttgtg cggatacttt attttcaaac
     3181 caaagcattc aggttcggca aggcaagagg cctaacatta ttttaatcct tgcagatgat
     3241 atggggtttg cggatattgg gtgttttggt tctgaaattg aaacgccgaa tctggacaaa
     3301 ttggctgccg aaggaatacg ctttacccag ttttgcaaca gtgctcgctg ctgtccaacc
     3361 cgagcttccc ttttgaccgg cctgcatcct catcaggccg gcataggctg gatgactgac
     3421 gattggggag ccagtaaacg agctgcatta aacagccctg cctataccga tgggctgaat
     3481 aaaaattgcg ttaccatagc agaggcactc aaacgcggaa gatatcatac cataatgtca
     3541 ggcaaatggc atgtc
//
LOCUS       DAOTBO010000193         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_21139_length_3541_cov_5.649455, whole genome shotgun sequence.
ACCESSION   DAOTBO010000193 DAOTBO010000000
VERSION     DAOTBO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21139_length_3541_cov_5.649455"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLE88_12900"
     CDS             <1..544
                     /locus_tag="PLE88_12900"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77405.1"
                     /translation="ATFALPEKDRLYYSKKYCLAELQVCFGGRVAEELFCDDITSGAQ
                     SDIKQATAIAREMVMNWGMGEELGPIKYAPDLGPESYFGIGPEYSQKTAEAIDREVKR
                     FIDEAYTKVRDLIEANKDKVEALAQALLKYETLDAEDVKLILSGQSLRKPTVSDLLQR
                     EQNKQHDDQAGPSETAGDSM"
     gene            complement(569..1255)
                     /locus_tag="PLE88_12905"
     CDS             complement(569..1255)
                     /locus_tag="PLE88_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOM77406.1"
                     /translation="MQTQQTILSLPLSSLLPHPANPNRLSEAAFAKLLRHIGRTGQYE
                     PLVVRRHPQTPGAYQILNGRHRARALKQLGYSHADCVVFEADDNAALVYLISLNRLSG
                     RDNLYKKSKLIEMLCRRFSSRQLAGLLPDSRTAIDKLAGLSASQPAPKTSTLLMPMTF
                     FVDPDQHALLLRAFEKARADVPSGTATQKRLQALLHIVGRFVESSPTQPAAQRAHPPQ
                     PAAQRHTAQG"
     gene            1285..1428
                     /locus_tag="PLE88_12910"
     CDS             1285..1428
                     /locus_tag="PLE88_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77407.1"
                     /translation="MQANPLRVSSRQDKDKKGAFSRRSGQRPRPAERLGSAGLCTRGV
                     HGV"
     gene            complement(1450..2502)
                     /locus_tag="PLE88_12915"
     CDS             complement(1450..2502)
                     /locus_tag="PLE88_12915"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOM77408.1"
                     /translation="MQTRYILFILVLLAVLLPLPACRRKAPAPQPADSNTPASVPAAR
                     IRPSAPVQRPDSQPAAAKGQIGADAAPLDGLVWLKGQPVRFQPQKVYVVEFWATWCGP
                     CRQSIPHLTQLQKQFKEKGVTVIGISNESAETVRSFVEKMGDQMDYTVAVDKDGKAGK
                     NYMEAYGQNGIPTAFIVDGAGKIVWLGHPLNGMDEVLAQVAAGTFDPSAWAKARAERE
                     ELERQIEELFRQYFSGLNSNQPSEQMRAIAGQIIEKAPADILNEFAWAILTEVEQPRR
                     DYAVALQAAQKANTLAEGKDASILDTYGLALFKNGSIAEAIAVQTKAVELAAGSEAML
                     SQFKARLEEFQAAAAQ"
     gene            complement(2548..3333)
                     /locus_tag="PLE88_12920"
     CDS             complement(2548..3333)
                     /locus_tag="PLE88_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77409.1"
                     /translation="MHPSDCVSILKYLTVAAGSRSDYEELAVFHYRNPSPGPIRFIYK
                     LIDQHPQRRLAVPAAGVIVYGPPAANLAIRNQVLGGFFAGLDRADGLALLNSGMLCIR
                     RVIIEPRYRGLGLASRLVKETLPLTQAAMVEAVSTMGCVHPFFVRAQMTEYICPPDVK
                     TSRLQAALETVGIDRSLWLDGQAVQAAIDALPDGQKAFVQLQIERFIQKFPRQRRLTD
                     TAQRLEFVLSKLTGSSRYYLWLNPEKSLPQQIFALQSAGSGVE"
BASE COUNT          714 a         1017 c         1081 g          729 t
ORIGIN      
        1 ggcgaccttt gccctgccgg aaaaagaccg gctctactac tccaaaaagt actgtctggc
       61 cgaactgcaa gtctgcttcg gcggacgggt ggccgaagag ctgttctgcg acgacatcac
      121 cagcggcgcc cagtcggaca tcaaacaggc caccgccatt gcccgcgaga tggtgatgaa
      181 ctggggcatg ggcgaggagc tgggacccat caagtacgct cccgacctcg gcccggaatc
      241 ctatttcgga atcgggccgg aatactccca gaagaccgcc gaagccatcg accgcgaagt
      301 caagcggttt atcgatgagg cctatacgaa ggtgcgggac cttatcgagg ccaacaaaga
      361 caaggtcgaa gccttggcgc aggcgctgct gaaatatgaa acgctggatg ccgaagacgt
      421 caaattgatc ctctccggcc agtcgctccg caagccgacg gtctccgacc tgcttcagcg
      481 ggagcagaac aaacagcacg atgaccaagc cggcccgtcc gaaaccgccg gcgattcaat
      541 gtaagtcctg ccggccggca ttacggcgtt atccctgcgc tgtgtgcctc tgtgccgccg
      601 gctgcggcgg atgcgcgcgc tgtgccgccg gctgggtggg actgctttcg acaaaccggc
      661 cgacgatatg cagcagagcc tgaaggcgct tctgggtagc cgtgccggac ggcacatcag
      721 cccgcgcttt ctcaaaggcc cgcagcagca gggcgtgctg gtccggatcc acaaaaaagg
      781 tcatcggcat cagcagcgtg ctggtcttcg gtgccggctg ggaagccgac aagccggcca
      841 gtttgtcgat ggccgtgcgg ctgtcgggca gcagaccagc cagctggcgg gaactgaacc
      901 gccggcagag catctcaatg agtttgcttt tcttgtagag attgtcccgt ccgctgagcc
      961 ggttgagact gatcagatac accagtgccg cattgtcatc ggcctcaaac accacgcaat
     1021 cggcgtggga ataccccagc tgctttaagg cgcgggcgcg gtggcggccg ttgagaatct
     1081 gatacgcccc gggcgtctgc ggatggcggc ggacgaccag cggttcatac tgcccggtcc
     1141 gcccgatatg ccgcagcagt ttggcaaagg ccgcctcact taaccggttc ggattggccg
     1201 gatgcggcag caagctggat aagggaagcg aaagaatcgt ctgctgtgtc tgcataaagg
     1261 ctcctttctg aaaatgcagg gaaaatgcag gcaaatcccc tgcgggtatc ttcacggcag
     1321 gacaaggata aaaagggtgc gttcagtcgg cgcagcggac agcgcccgcg gccggctgaa
     1381 cggctcggtt cggcaggtct gtgcacacgg ggtgtgcacg gcgtctaaac aggcatcgaa
     1441 aacgggcggc tattgtgccg cggcggcttg gaactcctcc agacgggctt tgaactggct
     1501 gagcatggct tcgctgccgg cggccagctc aacggctttg gtctggacgg cgattgcttc
     1561 ggcgatgctg ccgttcttaa acagcgccag cccataggta tccagaatgc tggcgtcttt
     1621 gccttcggcc agcgtgttgg ctttttgagc ggcctgaagg gctacggcat aatcgcgccg
     1681 gggctgttcc acttcggtca gaatcgccca tgcaaattcg ttgaggatgt cggcgggggc
     1741 cttctcgata atctggccgg ctatggcgcg catctgctca gacggctgat tgctgttcag
     1801 cccgctgaag tactgtcgga acagttcctc gatttgacgc tccagctcct cgcgttcggc
     1861 gcgtgctttg gcccatgccg aggggtcaaa ggtgccggcg gccacctgcg caaggacttc
     1921 gtccatcccg ttgagcggat gtcccagcca gacgattttg ccggccccgt ccacaatgaa
     1981 agccgtgggg atgccgttct ggccgtaggc ctccatataa ttctttccgg ctttgccgtc
     2041 tttgtccacg gcgacggtgt aatccatctg gtcgcccatt ttttccacaa acgaccggac
     2101 ggtttcagcg ctttcattgg aaatgccgat gacggtaacg cctttctctt tgaactgctt
     2161 ctgaagctgg gtcaggtggg gaatgctctg gcggcaaggt ccgcaccaag tggcccaaaa
     2221 ctccactaca tagaccttct gcggctggaa gcggacgggc tgtcccttca gccagacaag
     2281 cccatccagc ggggcagcgt cggcgccaat ctggcctttg gcggcggccg gctgcgagtc
     2341 gggtctttgg accggggcag agggcctgat gcgtgcggcc ggcacgctgg caggggtgtt
     2401 gctgtcggca ggctgcgggg caggcgcctt tctgcggcag gccggcaagg gcagcaggac
     2461 agcaaggaga accagtataa acaggatgta tcgggtctgc atagagtctt atcctttctt
     2521 ttctgtcttg ggtaattggc tattctatca ctctacgccg ctgccggctg attgcaaagc
     2581 aaaaatttgc tgcggcagcg atttttcggg attgagccac aggtaatacc ggctggagcc
     2641 ggtcagtttc gacagaacaa actccagccg ctgggctgtg tctgtcagcc ggcgctggcg
     2701 ggggaacttc tggataaacc gctcgatttg cagctgcaca aaggccttct gcccgtcggg
     2761 cagggcgtcg atggccgcct gcacggcctg tccgtccagc cacagcgacc ggtcaatgcc
     2821 gacggtttca agggccgctt gcagccggct tgtcttgaca tcgggcgggc agatgtactc
     2881 ggtcatttgc gcccggacaa aaaacggatg cacgcagccc atggtgctaa cggcttccac
     2941 cattgccgct tgggtcaggg gcaatgtttc ttttaccagc cggcttgcca gcccaagccc
     3001 gcggtagcgc ggttcgatga tgacccgccg gatgcacagc ataccggaat tcagcagggc
     3061 cagcccgtcg gctctgtcca gcccggcaaa aaagcccccc agcacctgat tgcggattgc
     3121 cagattggcc gccggcgggc catagacaat cactccggca gccggcaccg ccagacgccg
     3181 ctgcggatgc tggtcaatca gcttgtagat aaaccgaatc ggacccgggc ttggattgcg
     3241 gtagtgaaag acggccaatt cctcatagtc gctgcggctg ccggcggcaa ccgtcagata
     3301 cttcaaaata ctgacacaat ccgatggatg catagacata tactccttcc tttttaacgg
     3361 attataggac cagaaaaaaa ttgtgaaaaa ttgggggaaa ttactttacc caatcggtat
     3421 ttttaccgat gttttatgta gatggacgag tcgttcatat aggtttttag aggctgcctt
     3481 gagaaattat tgacctttga ataactaatg gaacgaatca tattttaagt ccggcttttt
     3541 t
//
LOCUS       DAOTBO010000194         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_21492_length_3487_cov_6.090618, whole genome shotgun sequence.
ACCESSION   DAOTBO010000194 DAOTBO010000000
VERSION     DAOTBO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21492_length_3487_cov_6.090618"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3487
                     /locus_tag="PLE88_12925"
     CDS             <1..>3487
                     /locus_tag="PLE88_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOM77410.1"
                     /translation="QPTWEAHFSATYNGTVTEPPIDPTNPPMSMRGIVYRNGEIRTFN
                     LEKYNMRGEHFEVILLDSDGVTKIPLDPGPVRTYRGWCEEEPDSYVEATLLPNGDLRY
                     HVFKGDADDWWYDPPYETNDTAAAINNFTLIGGTPVHPSGTAWPGAAFTDAAASLDSY
                     YKTVYQADFGYDVLVEYVNAANFTNEQSYARKAENAMGHYNAIYTRDILLECKLNKVV
                     YRTSNTGLDRTDTKWGIDWWNVNNYWNAMFPDVDHHFIGMVGSVGGGVAFVCDYGGTD
                     WAARSFNGWNSDKNWWHVARHEHGHNLGAGDYDGNRAEGATIMNGNNISLSRLSNAEV
                     NNIMNCRRNRAVSAKLRNIGPFAYPVPPYAKLDNFTAYAGIQRTLDILGNDYDANNDQ
                     IALADFPNTTPLGGTLEFSAGTGPNGRDELLYTPPAGQFGTDNFKYSIVDATGRTARG
                     YVAITVDIPTQTIRGHWKLDETTGTTAADSTINGKHGTLTGGITFDNNAAAGQFAGGL
                     TFDGTDDYINLPSLNFTSNTVTITAWIKPDKVQNEYAAIFSNRDGGAANLNFKTNNQL
                     GYHWNDANWTYNSGLSTPVDQWTFVALVIEPNKATFYTSNGTTLNKVIRNAVHNPESF
                     YGSPSIGRDDNGQSSRYFSGAMDDVRIYGIALSENEIRGVIGGGRAESPNPFDNAADI
                     PLLTNLRWSMGSSAVNNDIYFGTDYQTIQNADLTSRVYKGRQPENIFNPGTLKPGKTY
                     FWRIDQITPNGTIIPGQVWRFTATTKRLGGITRQVWQNIGTGVSVSDLTNNANYPDNP
                     SFSEIISSFEAPTNWADAYGSRIHGFIVPPATGSYTFWIAGDDNCQLWLSTDKNPGNA
                     VKIAYIEGNPAWTDSRQWTKYASQKSSPILLTGGMPYYIMALQKEGSGGDNLAVAWEG
                     PGISQQIINGAYLAPYAPDYNWGPLFETDPIVVDTIPEGYSFTLSLAGVANALDGGAI
                     TYHKAGGPLWLKESDDMTLEGIPGDGDVGTNLFTITATDSHGQSNYVNLKVNVVNTFT
                     GEAGLLDFAALASRWMDSNCQDIPACGGADLTGDCAVNAADIVSMASMWLMKRTYGGL
                     VAAWPFDADASDIISGHNGTLLNGAAIDSSQSVIGSGTLNLDGIDDYVVVPGYKGISG
                     TASRTCSVWIKTIKPG"
BASE COUNT          893 a          931 c          878 g          785 t
ORIGIN      
        1 cagcccacct gggaagcgca tttttctgca acctataacg gcacagttac ggagcctccc
       61 atcgacccga ctaatccccc gatgtcgatg aggggcatcg tatatcggaa tggtgaaatt
      121 cggacattca atctggaaaa atacaacatg cggggtgaac acttcgaagt cattctgttg
      181 gacagcgatg gtgttacaaa gataccgctg gatcccggtc cagtgcgtac ctaccgcggt
      241 tggtgcgagg aagaaccgga cagctatgtt gaagctactc tgctgcctaa cggggatctg
      301 cgctaccatg tctttaaagg agatgctgac gactggtggt atgatccgcc ctatgaaacc
      361 aacgataccg ccgccgcgat caataatttc actcttatcg gcggcacccc tgtacaccca
      421 tccggaacgg catggcccgg cgcagcattc acagacgctg cagccagctt ggatagttac
      481 tacaaaactg tttaccaagc ggatttcggt tatgatgtat tggtggagta tgtcaatgcg
      541 gcaaatttta caaatgagca aagctatgcc cgcaaagccg aaaacgctat gggacattac
      601 aatgccattt atacccgcga tattctgctt gagtgcaaac tcaacaaggt tgtgtaccgt
      661 accagcaata caggactgga ccgcaccgat acaaagtggg gcattgactg gtggaatgtc
      721 aacaattact ggaatgcaat gttccctgat gtagatcacc atttcatcgg aatggtcggg
      781 tctgtaggcg gcggtgtagc atttgtatgt gattacggcg gcacagactg ggcagcccgt
      841 tctttcaatg gctggaatag cgacaaaaac tggtggcatg ttgcccggca cgaacacggt
      901 cacaatctgg gagccggcga ctatgatggg aatagagccg agggggccac cattatgaac
      961 ggcaataaca tcagcctgtc ccgcctgtcc aatgcggaag tcaataatat tatgaactgc
     1021 cgacgcaata gggccgtttc ggcgaaactt cgcaacatag gtcccttcgc ttatccggtg
     1081 cccccctatg ccaaacttga caattttacc gcctacgcag gtatccagag aactctggac
     1141 attttgggta atgattatga tgccaacaat gatcaaattg cccttgcaga ttttccaaac
     1201 acaaccccct tgggcggaac tcttgaattt tccgccggaa caggaccaaa cgggcgtgac
     1261 gaactgcttt acacgccgcc ggcaggacaa ttcggcacag ataatttcaa atattctatc
     1321 gtagatgcta ccgggcgaac ggcacgcggt tacgttgcta tcaccgtaga tattcctacc
     1381 caaaccatac gcggccattg gaaactggac gaaacaaccg gcaccacagc tgcagattcg
     1441 actatcaatg gcaaacacgg tacgctgacc ggcggtatca cttttgataa caatgcagcc
     1501 gctgggcagt ttgccggcgg attaactttc gacgggacag acgattatat caacctgccc
     1561 agtttgaatt tcaccagcaa taccgtcaca atcaccgctt ggattaaacc cgacaaagta
     1621 caaaatgaat atgcggccat tttttccaac cgcgacggcg gggctgctaa cctcaacttt
     1681 aaaaccaaca atcaattggg ctatcattgg aatgatgcaa actggaccta caactccggt
     1741 ttatccactc ccgtagacca gtggactttc gtggcactgg ttattgaacc gaataaggct
     1801 actttttaca cctccaacgg cacgactcta aacaaagtca tcagaaatgc tgtacacaat
     1861 ccggaatcct tttatggatc gccgtcgatc gggcgggatg acaacgggca atccagcaga
     1921 tatttcagcg gagcaatgga tgatgtgcgc atttacggca tagctttatc cgaaaacgag
     1981 attcggggcg tcatcggcgg cggccgtgca gaaagtccca atccctttga taatgccgct
     2041 gatatcccgc ttctaactaa tttgcgctgg tcaatgggat ccagtgcagt caacaatgat
     2101 atctatttcg gcaccgatta ccagactatc cagaacgctg acctcacctc ccgggtctac
     2161 aaaggccgcc agccggaaaa tattttcaat ccgggtactc tcaagccagg gaagacctac
     2221 ttctggcgca ttgaccagat caccccgaac ggcacaatca ttcccggaca ggtctggcga
     2281 tttacagcca cgacgaagcg actcggcggc atcacccggc aggtgtggca aaacatcggc
     2341 actggtgtca gtgtgtccga cctaacaaac aatgcaaact atccagacaa ccccagcttc
     2401 agtgagatta tcagctcctt tgaggcccca acgaactggg ctgatgccta tggtagccgg
     2461 atccacggtt ttattgtccc gccagcaacc ggcagctata ctttctggat cgctggcgat
     2521 gacaactgtc aactctggct cagcaccgat aaaaatccag gcaatgctgt caagatcgca
     2581 tacatagaag gcaatccggc ttggacagat tcacgccagt ggaccaagta tgcttctcaa
     2641 aagtcttcgc cgatactcct gaccggcggt atgccctact atatcatggc tcttcagaag
     2701 gaaggttccg gcggtgacaa tctggcagtg gcctgggagg gtccaggcat cagtcagcaa
     2761 atcatcaatg gggcttattt ggcaccatat gcccccgatt acaactgggg gccgctgttt
     2821 gagactgatc cgattgtagt cgatactatt ccggaggggt attcttttac cctttcgctg
     2881 gctggagtag ccaatgcact ggacggcggc gcaataacct atcacaaagc cggcggaccg
     2941 ctgtggctga aggaatccga tgatatgacg ctggaaggca tccccggcga cggtgatgtc
     3001 ggaaccaatc tgttcacaat tactgccaca gacagccacg gacagtcaaa ctatgttaat
     3061 ctgaaagtca atgttgtaaa cacctttacg ggcgaagcgg gtctgctgga cttcgctgct
     3121 ctggcttccc gctggatgga tagcaactgt caagatatcc ctgcctgcgg cggagccgat
     3181 ctgaccggtg actgtgcagt caatgctgcc gacatcgtgt ctatggccag catgtggctt
     3241 atgaagagga cctatggcgg ccttgttgcg gcctggcctt ttgatgcaga cgcttcagac
     3301 atcatctccg gtcataacgg cacgctgctc aatggagctg ccattgacag cagtcagtcg
     3361 gttatcggca gcggcacgct gaatctggac gggattgatg attatgttgt ggtgcccggc
     3421 tacaagggca taagcggcac cgcttcccgc acctgcagcg tctggatcaa gacgatcaag
     3481 cccggct
//
LOCUS       DAOTBO010000195         3431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_21885_length_3431_cov_5.950237, whole genome shotgun sequence.
ACCESSION   DAOTBO010000195 DAOTBO010000000
VERSION     DAOTBO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21885_length_3431_cov_5.950237"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLE88_12930"
     CDS             complement(<1..250)
                     /locus_tag="PLE88_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77411.1"
                     /translation="MEPIAYVDLVSAAAALAGAFFVLFGWRRKHLSAGVPILLLVVLL
                     LTAFYGFGLFLEWSGLAGRIQWNFFENFGGVLVAFVWAF"
     gene            complement(250..696)
                     /locus_tag="PLE88_12935"
     CDS             complement(250..696)
                     /locus_tag="PLE88_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77412.1"
                     /translation="MTAPRNHTTISRRHPLLLGAQIYIFVLMILGAAALMANNIPEPP
                     ETEQPPAWEKIFYNPHDEAIMLLICLGIIPIVAVNFRHLRKLPDTGLLLASFGCMSGS
                     AVLTVAEGFVFPAALNWGEHLLLACSAVLLAVWCGRMAFGSRQEGF"
     gene            1732..2178
                     /locus_tag="PLE88_12940"
     CDS             1732..2178
                     /locus_tag="PLE88_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77413.1"
                     /translation="MTMHPVLMSGLWQDRRRDCRRKAEELAAALQQQAWLFSPAQKAL
                     LEMILDKGSSLEQIARLTGQNASTLSRQFDRLMRRLTDPLIRDVLRLGDKLSPLEAAI
                     LRDYFLCGMSQKAVIGKLGCSRYRLRKTLDRIRRPAAGKPKILKTA"
     gene            2194..2859
                     /locus_tag="PLE88_12945"
     CDS             2194..2859
                     /locus_tag="PLE88_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM77414.1"
                     /translation="MYDITINQQIDWQEPQTPKARQLMHLFGLRPVQLGKRRLQHTCR
                     LSLRPGDIVYITGASGAGKTVLLNAIYQQIPDNQRIRLDDIPIETGRPLIDCVDDRSL
                     FDALEILSRAGLSDVFCALQPPDQLSEGQKTRYRLARALMSSRTVLFADEFTSSLDRI
                     TAAVIANRLRWAAGQTQRIFVLASCHEDILPDLLPDILIVKYLTGKTETFYRDKRRGT
                     GLN"
     gene            complement(2996..3295)
                     /locus_tag="PLE88_12950"
     CDS             complement(2996..3295)
                     /locus_tag="PLE88_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77415.1"
                     /translation="MDMLKKMKGAKEQGGYVCPFMEQCHFLLSNYETMPELVERTVEH
                     YCTNSQARCARLELYESLGAAAVPELMLPNQYDWARQIVEDALDSSTVLPERRKS"
BASE COUNT          824 a         1022 c          858 g          727 t
ORIGIN      
        1 gaaacgccca cacaaaagcc accagcaccc cgccgaagtt ttcaaaaaag ttccattgaa
       61 tccgtccggc cagacccgac cattccagaa acaagccgaa accgtaaaag gccgtcagca
      121 gcagcaccac cagcagcaga atcggcaccc ctgccgataa gtgcttccgc cgccagccga
      181 acagcacaaa aaacgccccc gccaaagcgg ccgctgcgct gaccaaatcg acataggcaa
      241 tcggctccat cagaagccct cctgccggct gccaaaggcc atccgcccgc accagacagc
      301 cagcagcacc gcgctgcagg ccagcaggag atgctcgccc cagttcagcg ccgccggaaa
      361 gacaaagccc tccgccaccg tcagcacggc cgagccgctc atgcagccga aactggccag
      421 caaaagcccc gtatccggca gcttccgcag atggcggaag ttgacggcga cgatgggaat
      481 gatgcccaga caaatcagca gcataatcgc ttcatcgtgg ggattgtaga aaattttctc
      541 ccacgccggc ggctgttcgg tctcgggcgg ttcgggaatg ttgtttgcca tcagcgcagc
      601 ggcgcccaag atcatcagta caaatatata aatctgggct cccagcagca gcggatgccg
      661 tcggctgata gttgtgtgat tcctcggtgc tgtcacagtc ctgtcctccg ctatctctgc
      721 gcccccggcg ccggcgtcca tcgcacggct ccggagcggc tgcaaaattg ttctgccggc
      781 tcaaaccctg ctgccggcag gcagctgtct gcgccaaccg caggcagtgt tgattatacc
      841 cgattcggcc caaatgcaaa caacaaaatg cccctgtgct cccagccatc cgcccggcag
      901 gtcaagtccc ctgccgcacg gatgctccgt ctttggcaga cctgccgctt atctttcaca
      961 cacacgtcac ccctttgggg ggactatagg ggggtatatt atatatatat aatatatata
     1021 tatattctgt ggctcatttt cgtaccagtt caacgctggt ataggctcac tttgtaccta
     1081 tttaactatg ggtataggct cactttgata ccagtagaac gcattagagg cacacctgcg
     1141 taccagttca acgctggttg aggctcgctg tggtgccagt tgaatcccat cgcggctcat
     1201 tttgtaccgg tacaacgccg gtttggtttc aggccggcat aagcgggggc ttgggaccaa
     1261 tgaatccgac gggaaaaatc aaaaagccag acctgcttcg tgcagaatct ggcttcggag
     1321 gagggtgatg aaaaagattt catctcttgg ccagaaatta taatgcaacg accgcctatt
     1381 tgttctatcg gtcaaataaa tatttcataa aaaaaatttc cccatcctgt gcttctgcca
     1441 aagggcattt cggataaatt catatgtatt tgaaaacaaa gcagttataa aatttctgcc
     1501 ggtttgctcc tgctgcggct ataaaaaaaa tccggcgggc cagactgttt ttaagccctt
     1561 tgttatgcgt gatttaggag acagcaaacc gcacaattta acaatctgtt caaaaaaaat
     1621 tttttttctc ttgactggcc gttttgatag gttaaactaa catttatgtt agatttatat
     1681 ataacatatt gttgatatat ttagaccgaa aaacggcaga aggagaaaac catgaccatg
     1741 catcccgttt tgatgtccgg cctgtggcag gaccggcgca gggattgccg gcggaaggca
     1801 gaggagctgg cggcagccct tcagcaacag gcgtggctgt tcagtccggc ccagaaagcc
     1861 ctgctggaaa tgattctgga caaaggcagc tcgctggagc agattgcccg cctgaccggc
     1921 cagaacgcct cgaccttaag ccggcagttc gaccgcctga tgcgccgatt gacagaccca
     1981 ctgattcgcg atgtgctgcg tctgggcgat aaactgtcgc cgctggaagc ggccattctg
     2041 cgggattatt tcctgtgcgg catgagccag aaggccgtca tcggcaaact cggctgcagc
     2101 cgctatcgcc tccgcaagac gctggaccgc atccgccggc ctgccgcagg caaacccaaa
     2161 atcctaaaaa ccgcctaata aggagaagcc gctatgtacg acattaccat caaccagcag
     2221 atagactggc aagaacccca aacccccaag gcccggcagc tgatgcatct gttcggtctg
     2281 cgtccggtgc agctgggcaa acgccgtctg cagcacacct gccgcctgtc gctgcggccg
     2341 ggcgacatcg tgtatatcac cggcgccagc ggtgccggca aaacagtcct gctcaacgcc
     2401 atctaccagc agattcccga caaccagcgg attcgtctgg atgacatccc catcgaaacc
     2461 ggccggcccc tgattgactg cgtggacgac cgctcgctgt tcgatgcgct ggaaatactc
     2521 agccgcgccg gcctgagcga tgtattttgt gcccttcagc cgcccgacca gctcagcgaa
     2581 ggccagaaga cccgctaccg cttggcacgg gccctgatga gcagccgaac ggtgctgttt
     2641 gccgatgagt tcaccagttc gctggatcgc attaccgccg ccgtcattgc caaccgcctc
     2701 cgctgggccg ccggccagac ccagcgcatc ttcgtgctgg catcctgcca cgaggatatc
     2761 ctgcccgacc tgctgcccga tatccttatc gtcaaatacc tgacgggaaa aaccgagacc
     2821 ttctaccggg acaagcggcg cggaacggga ctcaattaag cgcagcaggt tctttgacaa
     2881 gttcatagag tttcggccgg cctgcttggc agccgacagg aaggaccggc ggacaggtcc
     2941 gctggatgtg ttttagggat gtgtcttgca gccagccggc tggaaacagc acctctcagg
     3001 actttctgcg ttcgggcagc actgtactgc tgtccaaggc atcttctaca atctgacgcg
     3061 cccagtcgta ctgattgggc aacatcagtt cgggaacggc agccgcaccc agcgattcat
     3121 aaagctccag ccgcgcacat cttgcttggc tgttggtgca gtagtgctcc accgtccgct
     3181 ccaccagttc cggcatcgtt tcgtaattgc tcagaagaaa atgacactgc tccataaacg
     3241 ggcaaacata cccgccttgt tcttttgcac ccttcatttt ctttagcata tccattgaag
     3301 ccatcctttc gaaatgtaaa tcaccaacaa aacagcctac caaattaact ggctccaatc
     3361 acttgggtta tacaactatt gacttttcat aaattaaagg aacgagcctt tgatgaccga
     3421 taataatata c
//
LOCUS       DAOTBO010000196         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_22066_length_3404_cov_3.540161, whole genome shotgun sequence.
ACCESSION   DAOTBO010000196 DAOTBO010000000
VERSION     DAOTBO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22066_length_3404_cov_3.540161"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLE88_12955"
     CDS             complement(<1..828)
                     /locus_tag="PLE88_12955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOM77416.1"
                     /translation="MHKTAETIDIGRIEAARQNAVIGRKLIVFKSTASTNDAAWRYAS
                     WPDHHGLCVLAEHQTRGRGRRGRRWHSQPGQSILCSILLLHQSIGAELLTLTAAVAAA
                     EAINRILQAQPHPPAGRAGPAACCIKWPNDILLSNKKAAGILVEQQTIRQQHNFVVGI
                     GINCSQTADFFDACRLGQPATSLAIETGRPVDRTAVVCEWLDQFEYWLGQSSAHTAVP
                     ADGTGSRDLFEQNPVIVKWLELSGMLGRRATVECNGKQYCGTCRGIHPAEGLILQLDG
                     "
     gene            complement(821..1207)
                     /locus_tag="PLE88_12960"
     CDS             complement(821..1207)
                     /locus_tag="PLE88_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77417.1"
                     /translation="MGWDVRPKSYDERFGWRRFDTDGCLTIDDTLVIIEVKISKPGTE
                     YGYWNAFSQGLIYSFQQKQDEPGKDFLVLCIILDWGRAAKRKLNECEKEILDLFRPHR
                     IYFLRVSMADKPFIEHNLNTDWSVIA"
     gene            complement(1549..>1728)
                     /locus_tag="PLE88_12965"
     CDS             complement(1549..>1728)
                     /locus_tag="PLE88_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77418.1"
                     /translation="WQLRHAVELRNKFGRKPLLEASGGITLNNVLAVASCGVDRISVG
                     AITHSAVAVDISLDR"
     assembly_gap    1729..1828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1829..2621)
                     /gene="nadC"
                     /locus_tag="PLE88_12970"
     CDS             complement(<1829..2621)
                     /gene="nadC"
                     /locus_tag="PLE88_12970"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOM77419.1"
                     /translation="MAGKKKTEMVRERVAPKNMKRLDMSKVEPLIDMAIQEDYGKGDP
                     TSELTVDAQARGRGFLVTREEIVVCGMGLAEAILKKYDKRLVLKTVIHDGERANVAER
                     LGIIEGPLRSMLSAERVVLNFLQRLCGISTMTWKFVSAVRGTKAKIYDTRKTTPGWRE
                     LEKYAVRCGGGYNHRMSLGDAVMFKDNHIADLGEKFEPKLAQMVAQARQYEHVKFVEV
                     EVDHVDDQLDRVLPIPGIDVVLLDNMGQWQLRHAVELRNKFGRKPL"
     gene            complement(2621..>3404)
                     /locus_tag="PLE88_12975"
     CDS             complement(2621..>3404)
                     /locus_tag="PLE88_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOM77420.1"
                     /translation="DQIQHLIGEIQKRIAAKERVLVTTLTKRLAEELSSFLSAKGIRC
                     RYLHSEIQTLERVDILRDLRLGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRS
                     ETSLVQTIGRTARHVNAAVVLYADTVTVSMKKAIDETRRRRTIQLAYNRQHGITPETI
                     RKEIKQGLAQQLRARKAAQQAIRLSETEYDRTELLAELEKEMLAAAEALEFEKAARLR
                     DQIQELKQMPELTSAGTNSRRQNNAAFSTNGRFRSGPRKAKQ"
BASE COUNT          611 a          983 c          901 g          809 t
ORIGIN      
        1 gccgtcgagc tgaagaatga gtccttcggc cggatgaatc ccccggcagg tgccgcagta
       61 ttgtttgccg ttgcattcca cggttgcgcg gcggcctaac atgccggaca gctccagcca
      121 tttgacaatc accggattct gttcgaatag atcccggctg cccgtcccgt ccgcagggac
      181 cgctgtgtgg gcgctgcttt ggccaagcca gtattcaaat tggtccagcc attcgcacac
      241 gacagccgtg cggtcaacgg gacggccggt ctcgatggcc agactggtgg ccggctggcc
      301 gagtcggcag gcgtcaaaga aatcggccgt ctggctgcag ttgatgccga tgccgacgac
      361 aaaattgtgc tgctgccgga tggtctgctg ttccaccaga atgccggccg cctttttatt
      421 gctgagcagg atgtcattgg gccatttgat gcagcaggcg gctgggcctg ccctgccggc
      481 cggcggatgc ggctgggcct gcaggattcg gtttatcgcc tcagcggccg ccaccgccgc
      541 cgtcagcgtc agcagttctg ccccgatgga ctgatgcagc agcaggatag agcacaaaat
      601 gctttgcccc ggctggctgt gccaccggcg cccgcgccgc ccgcgcccgc gggtctggtg
      661 ttcggccagc acgcacaggc cgtggtggtc cggccaggaa gcataccgcc atgcggcgtc
      721 attggttgaa gccgtggatt tgaagacaat aagtttcctg ccgatgacag cgttttgccg
      781 ggcagcctct atccggccga tatcaattgt ctctgccgtc ttatgcaata acgctccagt
      841 ctgtattcag gttgtgctca ataaacggct tgtcagccat gctgacccgc agaaaataaa
      901 tgcgatgcgg acgaaataaa tcgagtatct ctttttcaca ttcattgagt tttcttttgg
      961 cggctcttcc ccaatcaagg ataatgcaca gtaccagaaa atcctttccg ggttcatctt
     1021 gtttttgctg gaaagagtat atcaagccct gtgagaacgc attccaatag ccgtattcag
     1081 taccgggctt tgaaattttt acttcgataa tgaccagtgt atcatcaatt gttaggcagc
     1141 catcagtatc gaaacgacgc caaccaaatc gttcatcata acttttcggt cgaacatccc
     1201 agcccaaagc aatttcataa aaattacctt ggtatttggt taaaagagct ttttcttcct
     1261 gtatcttggg attgctttcc ttgtactcat tgaatttttc tggccaccga cgaaggaagt
     1321 tgaaaatcgt actctttctg gtttctatat tgctgattat tgtgtcattt acgccgttta
     1381 tacttttgtc tccttcggca cgataaagcc attgaaaaca gttataaatg cacccggccg
     1441 gcctatcccg gcttccattg gaaggggcgc aaagcatgcc tttgggcggc tgttttattg
     1501 tcagccccag ctgtgccggc gaaaagtcgc cgcttgctgg ggattgtctt aacggtccag
     1561 tgagatatcg acggccaccg ccgaatgggt aatagcgccg acggagatgc ggtccacgcc
     1621 gcagctggcc acggccagca cattgttgag cgtaatgccg ccgctggctt ccagcagggg
     1681 cttgcggccg aatttgttgc gcagctcgac ggcatgccgc agctgccann nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aggggcttgc ggccgaattt gttgcgcagc
     1861 tcgacggcat gccgcagctg ccactggccc atgttgtcca gcaggaccac atcaataccc
     1921 gggatcggca gaacgcggtc gagctggtcg tccacgtggt cgacttcgac ttcgacaaat
     1981 ttgacatgct catactggcg ggcctgtgcc accatctgcg ccagcttggg ctcaaatttt
     2041 tcgcccaagt cggcaatgtg attgtccttg aacatcaccg catcgcccag actcatccgg
     2101 tggttgtatc ccccgccgca gcggacagca tatttttcca gttcccgcca gccgggggtg
     2161 gttttgcggg tgtcgtagat tttggccttt gtgccgcgga cggcgctgac aaacttccac
     2221 gtcatcgtgc tgatgccgca gagccgctga agaaaattga gcaccacccg ctcggcgctg
     2281 agcatcgacc gcagagggcc ttcgataatg cccagccgtt cggccacatt ggcccgctcg
     2341 ccgtcgtgaa tcacggtttt cagcacaagc cgcttgtcgt attttttaag gatggcttcc
     2401 gccagcccca ttccgcagac aacgatttcc tcgcgggtca ccagaaagcc gcgtcccctg
     2461 gcttgggcat ccaccgtcag ctcgctggtt gggtcgccct tgccgtaatc ttcctgaatg
     2521 gccatatcaa tcagcggttc gaccttgctc atatccagcc gcttcatatt tttcggggcc
     2581 acccgttccc gtaccatttc tgtcttcttt tttccggcca tcactgtttt gcctttcttg
     2641 ggccgcttcg gaagcggccg tttgtactga aggcggcatt gttctgccgt ctgctgtttg
     2701 tgccggcgct ggtcaattcc ggcatctgtt tgagttcctg tatctggtcc cgcagccggg
     2761 cggctttttc aaattccagc gcctccgccg ccgccagcat ctctttttcc agttccgcaa
     2821 gcagttccgt ccggtcatat tcggtttcgc tcagacggat ggcctgctgg gcggccttac
     2881 gggcccgaag ctgctgggcc agtccctgct tgatttcctt gcggatggtc tcgggggtaa
     2941 tgccgtgctg ccggttgtac gccagctgaa ttgtacgcct tcggcgggtt tcgtcaatgg
     3001 cttttttcat cgaaaccgtc accgtatcgg catacagcac aacggccgca ttgacatggc
     3061 gggctgtccg tccaatcgtc tgcaccagcg aagtctcgct ccgcaggaag ccctccttat
     3121 cggcatcgag gatagccacc agcgacacct ccggcaagtc cagcccctcg cgcagcaggt
     3181 tgatgcccac cagcacatcg aattccccca gccgcagatc ccgcaggata tccacgcgct
     3241 ccagcgtttg gatttcgctg tgcaggtagc ggcagcggat gcctttggcc gacaggaagg
     3301 acgacagctc ttcggccaga cgcttggtca gggtcgtcac cagcacccgc tccttggcgg
     3361 ctatccgctt ttgaatctcg ccgataaggt gctgaatctg gtcg
//
LOCUS       DAOTBO010000197         3401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_22095_length_3401_cov_3.391811, whole genome shotgun sequence.
ACCESSION   DAOTBO010000197 DAOTBO010000000
VERSION     DAOTBO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22095_length_3401_cov_3.391811"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLE88_12980"
     CDS             complement(<1..1098)
                     /locus_tag="PLE88_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOM77421.1"
                     /translation="MRKVFLSIEQNRGYGRGLISGITHYSKLYGPWNFYTGTPFYYRQ
                     SKKESSAIDVIKRWCPDGIIMREDSDIDKIMNLGIPAIFITYTRMEIPGFITLLGDHE
                     VCGKLAAEHFLGRGFRNFAYCGIPSQYWSVCRGQSFQSRLAQAGFTVDIFPFSASETK
                     INWLKDQEKLKKWLIGLPKPVGLMTCTDDRAQNVIEACKACHLRIPEDIAIVGVDNDE
                     LLCSLMNPPLSSVALDAFKAGFEAAEILDRLMSRRLYKGSGIIIAPATHVVVRQSSDI
                     FAVEDPDVRAALKYISDNAKKAIQVRDVANACGLTIRTIQKKFRYYVGKSVSEEIDRT
                     RIELICQLLIESPKTINQIAHDIDFISENHFS"
     gene            complement(1211..3133)
                     /locus_tag="PLE88_12985"
     CDS             complement(1211..3133)
                     /locus_tag="PLE88_12985"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOM77422.1"
                     /translation="MKFVKILLVVLGLLVCQAAKAEGLEVCRLKSPDGLNAIRIWLNE
                     DGQPFYRVYRREIIFVQDSPMGLRCEGEDFKQGLRLHQIGSEESWHESYRLLAGNRLA
                     VDTRLNRRTLTLKNDKGSLVHIELAAGNEGIGFRCRFEESDALRLIHEELTGFWIPPA
                     AAGWLQPYHAAGRYTPAYEDFYFRVEPGDPPPVSREKPRGWSLPALFQMPSAAGWMLL
                     AESGTDGSYCACHLDADAKLKGLYRIAFAFDDEATGAGRFNPDAKPAPVLTRSTPWRV
                     MIMGDEAKDILQSTLITDLAGPSQIEDASWIEPGRASWSWWSHPERPDTADRYNRFTD
                     LAARFGWEYTLFDAGWWDTDLAAVCRYAHDKGVKPLLWAHAVDFYDARKRRQKLDQWA
                     AAGIRGIKVDFWCSDRQETIAAMLATLKDAAERKLVVNFHGCTIPRGWHRTWPNLLTA
                     EAVLGTECYFFEERYPQKAAELNTILPFTRNVLAPMDVTPVALTMRKFPRKTTAAHEL
                     AAALTGGSGIIHYADSDEVFNSFPEGVKQVLKDAPAHWDETICLLGEPGRTVVLARRA
                     GQKWFIAGLNGTAQPRLVTLDLKSLGQFPACIEITEGNENPLMEFSVERITDVSGWEH
                     QVPPYGGFVLESMPAE"
     gene            complement(3175..>3401)
                     /locus_tag="PLE88_12990"
     CDS             complement(3175..>3401)
                     /locus_tag="PLE88_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77423.1"
                     /translation="IFQYFAPQLHFLIQVAVTFQLVLLIMILFTFFAPLQQPRPLPVR
                     QDIVLKTALDVKIWGSLVIAAVAVFYVVFR"
BASE COUNT          875 a          998 c          756 g          772 t
ORIGIN      
        1 ggaaaagtgg ttttcactga taaagtctat atcgtgtgca atctggttaa tcgtcttggg
       61 cgattcaatt aacaactggc aaatcaattc gattcgagtc cggtcaattt cctcgcttac
      121 agacttgccg acataatacc tgaacttttt ctggattgtc cggattgtta aaccgcaggc
      181 atttgcgaca tcacgcacct gaattgcctt cttggcattg tcggatatat atttcaatgc
      241 cgcacgaaca tccggatctt ctaccgcaaa gatatcggag gactgccgaa ccacaacatg
      301 agtggctggg gctatgataa tgccgctgcc tttatataaa cgcctgctca ttagccgatc
      361 caagatttca gccgcctcaa agcctgcctt aaacgcatcc aaagcaacac tggacagcgg
      421 cggattcatc agactgcata acaattcatc attatcgact cccacaatcg ctatatcttc
      481 gggtatccgc aaatggcacg ccttgcacgc ttcaatgaca ttttgcgcac ggtcgtccgt
      541 acaggtcatc agtccaacgg gcttgggcaa acctatcagc catttcttca gcttttcctg
      601 atccttcagc cagttaattt tcgtttcgga tgccgaaaaa ggaaaaatgt caaccgtaaa
      661 tccggcctgg gcaagccggc tctggaaact ttggccgcgg caaacagacc agtactggct
      721 gggaatcccg cagtaggcaa aattccgaaa accccgaccc aaaaaatgtt cggctgccag
      781 ctttccgcac acctcgtggt cgcccagcaa agtaatgaat cccggaattt ccatgcgggt
      841 gtaggtgata aagatggccg ggattccaag attcataatt ttatcaatgt ctgaatcctc
      901 acgcatgata atgccatctg gacaccatcg tttaatcaca tcaatcgctg aggattcctt
      961 tttggactgc cggtaataga acggcgtgcc ggtataaaaa ttccaagggc cgtacagttt
     1021 tgaataatgc gtaataccgc tgattagacc ccgtccatat cccctgtttt gctcaattga
     1081 taaaaatact tttcgcatcg atttccttga aattctctca taccgacatg accgtcatta
     1141 taacttacag ccaagataat gtacaatttc cccaacatgc ttatccgccg aagacaggaa
     1201 caatcctgcc tcactcagcg ggcatcgatt ccaatacaaa tccgccatac ggcggcactt
     1261 gatgttccca ccctgaaaca tctgttatcc gttcgacaga aaattccatc agcggatttt
     1321 cgttgccctc ggtgatttca atgcatgcag gaaattgtcc cagagatttc agatccagcg
     1381 taaccagcct cggttgagca gtcccattca gacccgcaat aaaccatttc tgcccggcac
     1441 gacgagccag aacaaccgtc cgtcccggct cgcccagcag gcaaatcgtc tcatcccaat
     1501 gtgccggggc atctttcaat acctgtttaa caccctctgg aaaactgttg aagacctcat
     1561 ccgaatcggc ataatgaata atcccggatc cgcctgtaag agccgccgcc aattcatgag
     1621 ccgccgttgt ctttctcgga aatttccgca tggtcaaagc caccggcgtc acatccattg
     1681 gggccagtac attccgggta aacggcagaa tggtattcag ttcagcggcc ttttgcggat
     1741 agcgctcctc gaaaaagtag cactccgttc ccagcaccgc ctcagctgtc agcagattcg
     1801 gccatgtccg atgccagccg cgaggaatcg tgcagccgtg aaaattgaca accagttttc
     1861 gctcggcggc atctttgagc gtcgccagca tggccgcgat tgtctcctgc cggtccgagc
     1921 accagaagtc cacttttatc ccccggatgc cggcggcagc ccactggtcc aatttttgcc
     1981 gccgcttccg ggcatcataa aaatccacag catgcgccca cagcagcggt ttgacgcctt
     2041 tgtcatgggc atagcggcaa acagctgcaa ggtccgtatc ccaccagccc gcatcgaata
     2101 aggtatattc ccaaccgaat cgggctgcca aatccgtaaa gcgattgtac cgatcggcgg
     2161 tgtctggacg ttcaggatga gaccaccacg accatgatgc acgccccggt tcaatccatg
     2221 atgcatcttc aatctgcgac ggcccggcca agtcggtaat caatgtggac tgcagaatat
     2281 ctttcgcttc atcccccata atcatgacgc gccacggtgt cgagcgggtc aggacggggg
     2341 ccggttttgc atccggatta aacctccctg cgccggtcgc ttcatcgtca aaagcaaagg
     2401 cgattcgata caaacccttt aacttggcat ccgcatccag atggcaggca caatacgagc
     2461 cgtcggttcc agactctgca agcagcatcc acccggcggc tgagggcatt tggaaaagcg
     2521 ccggcagact ccagccgcgc ggtttttcac gggaaaccgg cggcggatcg cccggttcaa
     2581 cgcgaaaata gaaatcttca taggccggtg tataacggcc ggctgcatga tagggctgga
     2641 gccagccggc cgccgcagga ggaatccaaa atcccgtcag ttcttcatgg atcagacgca
     2701 acgcatcgct ttcttcaaag cggcagcgaa aaccaatccc ctcattgcct gctgccagct
     2761 cgatatgcac cagcgacccc ttgtcatttt ttaatgtcag cgtccgccga ttcagccggg
     2821 tatcaacggc taatcgattc cccgcaagaa gccggtatga ctcatgccaa gattcttcag
     2881 aacctatctg gtgcagtctc aatccctgct taaagtcctc tccctcgcag cgcagcccca
     2941 tcggcgaatc ctgaacaaag ataatttccc tgcgataaac tcggtaaaac ggctgtccgt
     3001 cctcatttaa ccaaatccga atcgcattaa gcccgtccgg ggacttcaac ctgcatacct
     3061 ccaacccctc cgcctttgct gcttgacaga caagcagccc cagaacaacc agcaggattt
     3121 ttacaaactt cattcagctt ctcctgtcac cgcccaacaa cagcgcagaa catcctaccg
     3181 gaaaacaaca taaaataccg ccactgccgc gataaccaga ctgccccaaa tcttcacatc
     3241 cagcgcagtt ttgagaacaa tatcctgcct tacaggcagc ggacggggct gctgcagcgg
     3301 tgcaaaaaag gtgaacagaa tcataatcag aagcaccagt tggaaggtca ctgctacctg
     3361 aatcaaaaaa tgcagctgcg gagcaaaata ctgaaaaatg c
//
LOCUS       DAOTBO010000198         3383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_22236_length_3383_cov_4.213942, whole genome shotgun sequence.
ACCESSION   DAOTBO010000198 DAOTBO010000000
VERSION     DAOTBO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22236_length_3383_cov_4.213942"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLE88_12995"
     CDS             <1..526
                     /locus_tag="PLE88_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77424.1"
                     /translation="VEKNEVVFYDVSYIEVTPRYNGRKVAVELTDFSVFYTQDTVDNV
                     AASLKNGKTKLVVEKGKIIKVTKSKAGEVKTELLTKHWTDWIDYWSVDFDFESKREII
                     RVQDPETGQFVEQWSGDYVFENEWQSFRTKKNRTLELKSIEHEYEKGKRYKIAVKVVD
                     IFGNDTMKIIEIGV"
     gene            530..3217
                     /locus_tag="PLE88_13000"
     CDS             530..3217
                     /locus_tag="PLE88_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOM77425.1"
                     /translation="MAIHRDFPTSPYVILDPAIRWFPADEALRETSYEKLLPPLVYKL
                     RREVADWRNKNYEGATATSKALLRWWFETEHLMPQADGTDVRFQYYFAQREAVETVIY
                     LYDAAKVKDKYDLLRFDSSGAVSTGMFQEDWRRFVIKMATGSGKTKVLSLILAWCYFH
                     KLYEAGSDLARNFLVIAPNIIVLDRLRADFDGLKIFYHDPVLPDNGYEGRNWRDDFQM
                     TLHIQDQVRVTHPIGNIFLTNIHRVYESSDIEPSYNDDNTMDYFLGKRWTGATTDSKV
                     DLGEIVRNVDELVVLNDEAHHIHNPKLAWFKSILDIHNKLKQKDKYLSLQVDVTATPK
                     HDNGAIFVQTVCDYPLVEAIWQNVVKHPVLPDGPSRAKLHETQSLKYVERYADYIHLG
                     VEEWRKAYAEHEKLGKKAVLFVMTDVTDSCDEVAEYLEAMYPELKGAVLTIHTNRTGD
                     ISESSTSGEAKKELDLLREQANKIDSLDSPFKAIVSVLMLKEGWDVRNVTTIVGLRAY
                     SARSNILPEQTLGRGLRRMYREGEVSEKVSVVGTEAFMEFVESIQSEGVELERASMGK
                     GTAPKTPMIVEVDKNNANKDLDKLDTQIPVLTARTVREYKRLEEIDLDKLQFEKVPYQ
                     QFSEEQQREIVFKDITTDDFSHTTVLDTLQIDDYRNVIGFFAQRIMKDLRLVSGYDVL
                     YGKMKTFIADYLFGQKVDLESRNTLRNLSEIQASKTVFDTFKKAINALTVVDKGSAEI
                     RDYIKLKQVRPFTVKDQEFYVPKKSVFNKIVGDSHLEIEFAAFLDACEDIISYAKNYF
                     AVNLRIDYVNADGDISNFYPDFIVKVSEKEIYIVETKGLEDLDVPLKMERLKQWCLDI
                     NAAQSQVRFDFVFVDEENFHRYNPKSFRSLVESFRDYKD"
BASE COUNT          951 a          677 c          924 g          831 t
ORIGIN      
        1 ggtggaaaag aatgaagtgg tgttttatga tgtctcgtat atcgaggtaa cgccgcggta
       61 caacgggcga aaggtggcgg tggaactgac agacttttcg gtgttttaca cgcaggatac
      121 cgtcgataac gtcgcggcct cgctgaaaaa cggcaagacc aaactcgtcg tggaaaaggg
      181 caagattatc aaggtaacca aaagcaaggc cggcgaggtg aaaacggaac tcctgaccaa
      241 acactggaca gactggattg actactggag cgtggatttt gactttgaaa gcaagcggga
      301 gattatccgt gtgcaggacc cggaaaccgg ccagtttgtt gagcagtggt ccggcgatta
      361 tgtgtttgag aacgagtggc aaagttttcg gaccaaaaag aaccgcaccc tcgaactgaa
      421 gagtatcgag cacgaatatg aaaagggcaa acggtacaaa atcgccgtta aggtggtgga
      481 tattttcggc aatgatacga tgaagattat tgaaatcggg gtgtaagaga tggccattca
      541 cagggatttt cccacatcgc cgtatgtgat tttagacccg gctattcgct ggtttccggc
      601 ggatgaggcg ttgagagaaa cttcatacga aaaacttttg cccccgttgg tgtataagtt
      661 gcgccgggag gtagccgact ggcgcaacaa gaactatgag ggggccacgg cgacaagcaa
      721 ggccctgctg cgatggtggt tcgagaccga gcatcttatg ccgcaggcag acggcacgga
      781 tgttcgcttt cagtattatt ttgcccaacg cgaggccgtc gagacagtga tttatttgta
      841 cgatgcggcc aaggtcaaag ataaatatga tttgcttcgc ttcgattcct ccggtgcggt
      901 ttcaacgggg atgtttcaag aggactggcg acggtttgtc atcaaaatgg caaccggcag
      961 cggcaagaca aaagtgctaa gtttgattct ggcgtggtgt tattttcaca aactgtatga
     1021 ggccgggtcg gatctggcgc gaaactttct ggtgattgcg ccgaatatta ttgtgctgga
     1081 cagactgcga gcagattttg acggtctgaa aatcttttat cacgacccgg tgctgccgga
     1141 caacggctat gaaggccgga actggcggga cgattttcag atgacgctcc atattcagga
     1201 ccaggtgcgg gttacacatc cgattgggaa tatctttttg accaacattc accgggtata
     1261 tgaaagcagc gacatagaac catcctataa cgatgacaat acgatggatt atttcctcgg
     1321 caaacgatgg accggggcaa cgactgattc gaaggttgac ttgggggaaa tcgtccgaaa
     1381 tgtggatgaa ctggttgtac ttaacgatga agcccatcat attcataatc cgaagctggc
     1441 atggttcaaa tcgattctgg atattcataa taaactgaag caaaaagaca aatacctgtc
     1501 tttgcaggta gatgttaccg ccaccccaaa gcatgataac ggagccattt ttgttcaaac
     1561 ggtttgtgat tatccgctgg tggaggccat ttggcaaaat gtagtcaaac atccggtatt
     1621 gccggatggc ccgagccgag caaaattgca cgagacacaa agtctgaaat atgttgagcg
     1681 gtacgccgat tatattcatc tgggcgtgga ggaatggcga aaggcctatg cagaacatga
     1741 aaaactcggc aaaaaagcag tgctgtttgt tatgaccgat gtaacggata gctgcgatga
     1801 ggtggcagaa taccttgaag cgatgtatcc ggagttgaag ggtgcagttt taacgattca
     1861 caccaataga actggcgata tcagtgagtc gtcgacatcc ggcgaggcca aaaaagaact
     1921 cgatcttttg cgcgaacagg ccaataagat tgacagtttg gatagtccct tcaaggcgat
     1981 tgtgtctgtc ctgatgctca aagagggctg ggatgtacgc aatgtaacga cgattgtggg
     2041 gttacgggcg tattcagcaa gaagcaatat cctcccggaa cagacattgg gacgcggatt
     2101 gagacggatg taccgggaag gagaagtatc cgagaaggtc agcgtcgtcg gcacagaggc
     2161 gtttatggag tttgtcgaat ccatccagtc ggaaggggta gaactggaac gtgcaagcat
     2221 gggcaagggt acagctccca aaacccccat gatcgttgaa gtcgataaga acaatgcaaa
     2281 caaagacctt gataagttgg acactcagat accagtgttg acagcgcgga ccgttcgtga
     2341 atacaagcgg ctggaagaga ttgatttgga taaacttcag tttgagaaag tcccttatca
     2401 gcaattttcg gaagaacagc agcgggagat cgtctttaag gatattacaa cagacgattt
     2461 ttcgcatacg actgtgctgg acaccctgca aatagacgat tatcgaaatg tgatcggctt
     2521 ctttgcgcag cggattatga aagacctgcg actggtcagc ggctatgatg ttttatatgg
     2581 caagatgaag accttcatag cggattatct gttcggacaa aaggttgatt tggaatcgcg
     2641 gaacacactt cggaatttat cagaaattca ggcaagcaaa acggtttttg atacattcaa
     2701 aaaggccatc aatgcattaa ctgttgttga taaggggtca gcagaaattc gagattatat
     2761 caaactcaaa caagtccgcc cctttacggt caaagaccag gaattttatg tgccgaaaaa
     2821 aagcgtgttt aacaaaattg tcggcgacag ccatttggag attgagtttg ctgcgttttt
     2881 agatgcctgc gaggatatca tctcttatgc aaaaaattat ttcgcagtca atctgcgcat
     2941 cgattatgta aacgcggatg gcgatatatc gaacttctat cctgatttta ttgtgaaggt
     3001 ctcggaaaaa gaaatttaca tcgtcgaaac aaaggggctg gaagatctgg atgttccgtt
     3061 gaaaatggaa cggctgaaac aatggtgtct tgacatcaac gccgcacaat cacaggttcg
     3121 cttcgatttt gtgttcgtcg atgaggagaa ctttcataga tacaacccaa aatcgtttcg
     3181 ctccctggtc gaatcattca gagattataa agattaacgg agggtcgaaa gcatcaggtg
     3241 ctctccctgc gatcatccct gccgaggggt ggggagtggg ggattgcagg tgggctttgt
     3301 ccggttcttg gcttgggggg gccaaattgg atagacattt tttgtattcg atgtatacaa
     3361 tttttgccgt tgctcatttt taa
//
LOCUS       DAOTBO010000199         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_22434_length_3358_cov_4.498032, whole genome shotgun sequence.
ACCESSION   DAOTBO010000199 DAOTBO010000000
VERSION     DAOTBO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22434_length_3358_cov_4.498032"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..483)
                     /gene="rplK"
                     /locus_tag="PLE88_13005"
     CDS             complement(58..483)
                     /gene="rplK"
                     /locus_tag="PLE88_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOM77426.1"
                     /translation="MAKEITGKIKLQAPGGQATPAPPIGPALGQHGVNIGQFVQQFNE
                     RTRELNGMTVPVEITVYSDRSFEFIVKSPPASVLLKKEAGLAKGSGVPNKEKVGAVTK
                     AQIRKIAEMKFKDLNAYDLEHADRIIEGTARSMGINIEG"
     gene            complement(527..1066)
                     /gene="nusG"
                     /locus_tag="PLE88_13010"
     CDS             complement(527..1066)
                     /gene="nusG"
                     /locus_tag="PLE88_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOM77427.1"
                     /translation="MQWYVLRVASNKEEQVRETLARKLQVEGVSGVGRIVVPTEQIKR
                     IRAGKQRVIKRKLYPGYVFMELEPKEDGRVPDDVWYIIKDTMGVGDFIGTEGVPTPMR
                     DTDVAKMLKEIERPEETPNIKVDFEKGDVVKIREGAFENFEGVVDAIDGERGMVRVIV
                     TIFGRSTPLDIEYWQIEKV"
     gene            complement(1102..1488)
                     /gene="secE"
                     /locus_tag="PLE88_13015"
     CDS             complement(1102..1488)
                     /gene="secE"
                     /locus_tag="PLE88_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOM77428.1"
                     /translation="MHLTIYKRGQGKNTRLWTALAVLGVAAIGCWQLHLKLSVYDNVW
                     LHTMTPALVLAVLAGLIYWLSNLPVIADFLIASEGEIKKVSWSSRKEIVNSTMIVIFV
                     VIVIAAGLGLVDLCFQIFFRQIVNLY"
     gene            complement(1551..1623)
                     /locus_tag="PLE88_13020"
     tRNA            complement(1551..1623)
                     /locus_tag="PLE88_13020"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1588..1590),aa:Trp,seq:cca)
     gene            complement(1631..1798)
                     /gene="rpmG"
                     /locus_tag="PLE88_13025"
     CDS             complement(1631..1798)
                     /gene="rpmG"
                     /locus_tag="PLE88_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOM77429.1"
                     /translation="MAKAQKREYVWLECKDCGDRNYRTEVNVQGGTPKFELKKYCKRE
                     RKHTVHKIRRK"
     gene            complement(1913..3130)
                     /gene="tuf"
                     /locus_tag="PLE88_13030"
     CDS             complement(1913..3130)
                     /gene="tuf"
                     /locus_tag="PLE88_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOM77430.1"
                     /translation="MAKDIFVRTKPHVNIGTIGHIDHGKTTLTAAITMRMFHKNGGKG
                     RVKRYDEIDNAPEEKERGITINTAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMD
                     GAILVVAASDGPMSQTREHILLARQVNVPRIVVFLNKVDQVDDPELIDLVEMELRELL
                     NKYEFPGDEIPIIRGSALQAMESEGKDDKACACIDELMKAVDSYIPVPQRATDQPFLM
                     PVEDVFSIKGRGTVGTGRIERGVVKVGDEVEIVGLADEVRKTTVTGVEMFNKTLEEGQ
                     AGDNVGLLLRGIEKKDLERGQVIAKPGSITPHTKFNAEVYVLTKEEGGRHTPFFPGYR
                     PQFFFRTTDVTGAIIEIKSREGKPVEMCMPGDNIEMTAEIISKIAMEEGLRFAIREGG
                     RTVGAGVVTKILA"
BASE COUNT          743 a          977 c          742 g          896 t
ORIGIN      
        1 tcgcgaaata aacttacttt ttcgctccca ctcaacaagc cgtggtcagc agaatagtta
       61 tccttcgata ttgatgccca tgctgcgcgc tgtcccttcg ataatccggt cagcatgctc
      121 caggtcatag gcgttaaggt ctttgaactt catttccgca atcttgcgaa tctgcgcctt
      181 ggtgacagcg ccgacttttt ccttgttcgg aacgccgctg cctttggcca gtcccgcttc
      241 ctttttcagc agcaccgatg ccggcgggct cttcacgata aactcaaacg atcgatccga
      301 gtaaaccgtg atttctaccg gcacagtcat cccattcaac tcgcgcgtcc gctcattaaa
      361 ctgctggaca aattgaccga tattgacgcc atgctggccc aatgccggac caatcggcgg
      421 agccggtgtg gcctgcccgc cgggggcctg taatttaatt tttcctgtaa tttcttttgc
      481 catgttaatt cccgccttgt tctatcaggt ttctttcaga aacaaactat actttttcaa
      541 tctgccagta ttcaatatcc agcggcgtgc tgcggccgaa aatagttaca ataacccgca
      601 ccatcccacg ctcgccgtcg atggcatcca ccacgccttc aaagttttca aacgcacctt
      661 cccgaatttt cactacatcg cctttttcga aatccacttt gatattcggc gtttcttcgg
      721 ggcgttcgat ttccttgagc attttagcca catccgtgtc gcgcatcggc gtcggcacac
      781 cttcggtgcc gataaagtct ccaacaccca tcgtgtcctt gataatgtac cagacatcat
      841 ccggaacccg gccgtcttct ttcggttcca gctccataaa cacatagccg gggtagagct
      901 tgcgtttgat gacccgctgc ttgcccgcac ggattcgctt aatctgctca gtcggcacaa
      961 cgatgcgtcc cacgccggaa acgccttcga cctgcagctt gcgggccagg gtttcgcgta
     1021 cctgttcttc tttattggag gcaactcgca atacgtacca ttgcataact gcgtattcct
     1081 ttggacagtt tgtagggccg attaatacag attaacaatt tgacggaaaa atatctggaa
     1141 gcacagatcg accaagccaa gaccggccgc aatcactata actacaaaaa tcactatcat
     1201 tgtcgaattg acaatctctt tcctgctgga ccagctgacc ttctttattt cgccttcaga
     1261 ggcaatcaga aaatctgcaa taacaggaag attgctcagc cagtaaataa gaccggccag
     1321 aaccgcaagc acaagggcag gcgtcatcgt atgaagccat acattatcgt acacgctcag
     1381 cttcagatga agctgccagc atccgatggc tgccacaccc agcaccgcaa gagccgtcca
     1441 cagccgagta ttcttaccct gtccgcgctt gtaaattgtt aaatgcatac tgtctatcgc
     1501 cttcttccca attgatttta tctgccgact ttccgaaatc tttcgtggaa caggtcagga
     1561 gggattcgaa cccccagcct gcggttttgg agaccgctgc tctgccaatt gagctactga
     1621 cctaattccc ttatttacgc cggatcttat gcactgtatg cttgcgttcc cgcttgcagt
     1681 attttttcag ttcaaacttc ggggtgccgc cttggacatt tacctccgtc cgatagttgc
     1741 ggtcaccgca gtctttgcat tccaaccaga catattcccg cttttgagct ttagccattg
     1801 ttaaggacct ctctgataaa acacagcgac ctttccgctg ctgctcagca tagaaaggac
     1861 ttgaaaagcg ccgtgccgca aagcacggcg ctttggactc cgatttataa ggttatgcaa
     1921 gaatctttgt aacgacgccg gcacctaccg tacggccgcc ttcacgaata gcaaaacgca
     1981 gaccttcttc cattgctatc ttggagataa tttcggcggt catttcaata ttgtcgcccg
     2041 gcatgcacat ctcaaccggc ttgccttcac ggctcttaat ctcaataatc gcacccgtca
     2101 catcggttgt gcggaagaag aactgcgggc ggtaacccgg gaagaacggg gtatgacggc
     2161 cgccttcttc tttggtcagc acatacacct cggcattaaa cttggtgtga ggcgtaatgg
     2221 agccgggctt ggcaataacc tgaccgcgct ccaagtcttt cttttcaatg ccccgcagca
     2281 gcagtccgac gttatcgccg gcctggcctt cttccagggt cttgttgaac atctcaacgc
     2341 cggtaacggt tgtcttgcgt acctcatctg ccaatccgac gatttcgact tcatcaccga
     2401 ctttgacaac gccgcgctcg atacggccgg tacctactgt gccgcggccc ttgatgctga
     2461 agacgtcttc gaccggcatc aggaacggct ggtccgtggc ccgctgggga accggaatat
     2521 aactgtcgac ggccttcatc agctcatcaa tgcaggcaca ggccttgtcg tccttgccct
     2581 ccgattccat cgcctgaagc gctgaaccgc gaataatcgg gatttcatcg cccggaaatt
     2641 catacttgtt gagcagttcc cgaagttcca tctcgaccag gtcaatcagt tcagggtcat
     2701 ccacctgatc gactttgttc aggaacacca cgatgcgcgg cacatttacc tgacgggcca
     2761 gaaggatatg ctcacgggtt tgagacatcg ggccgtcgct ggccgccacc accagaattg
     2821 caccatccat ctgggccgca ccggttatca tattcttaat atagtcggca tgtcccggac
     2881 agtccacgtg ggcatagtgc cggttttcgg tctcatactc cacgtgggcg gtattgatgg
     2941 taataccgcg ttccttttct tccggagcat tgtcgatttc gtcataccgt ttcacccggc
     3001 ccttgccgcc gttcttgtga aacatgcgca tcgtgatggc agccgtcaat gtcgtcttgc
     3061 cgtggtcgat atgaccaatc gtaccaatgt tcacatgcgg ttttgttctt acaaagatat
     3121 cctttgccat gtgttaataa cctcctgaat attcctgttt cttaaggtta acttgtattg
     3181 tttgattcaa aactacatct tcaactacat atccttaatc tgggctggac aatactctct
     3241 gcttttgcaa tcgctgcacc atccaagcaa ggtaaatcaa accggccctg actgttttgc
     3301 cttagctgct gatgggattt gaacccatga cctcgtcctt accaaggacg cgctctac
//
LOCUS       DAOTBO010000200         3347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_22534_length_3347_cov_5.748785, whole genome shotgun sequence.
ACCESSION   DAOTBO010000200 DAOTBO010000000
VERSION     DAOTBO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22534_length_3347_cov_5.748785"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLE88_13035"
     CDS             <1..193
                     /locus_tag="PLE88_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOM77431.1"
                     /translation="AEKEKQCQLLAAELQSTRRRVNVLEHVVIPETKETIKYIYDKLG
                     EAERDNISRLMKIADIIRR"
     gene            complement(213..1616)
                     /locus_tag="PLE88_13040"
     CDS             complement(213..1616)
                     /locus_tag="PLE88_13040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77432.1"
                     /translation="MGTKTLIVVAFLISSITAWTQSDQFAMAVDFLRAQPYGEVYILL
                     GQVLLVINLSAFLWRVILFFRYRPVEPCSDSQLPTCTVVVPAYNEGSMVFKTLESVLQ
                     SDYPADKLQIIAVDDGSADDTWEWMKKAAAQSGGRIEAVRLAKNGGKRRALYEGFIRS
                     RAEVLVTIDSDSLINPDTLRCLVSPFAADEKIGGVAGNVAVLNRHKDIIPRMLEVVFA
                     YSFEFVRASQSAVNSVFCTPGALSAYRRTAVMKVLDAWLNQTFLGRPAGIGEDRAMTN
                     AILQQGLHVVFQSNAVVYTTVPVRYKGLCKMFLRWARSNVRETIAMAGFIFTRFRQTP
                     ALGARVNFVLSVIQMIVPQILLAGLTGCILWHPALFISHTLLGACLTGTVPAAFYAWR
                     RQYSSEALWAILYAMFWIFGLSWITPYALVTVGNNKWLTRDVKRKRGSLRRGRLMLHL
                     FRRAAYATVHTLAIRRF"
     gene            complement(1955..3136)
                     /locus_tag="PLE88_13045"
     CDS             complement(1955..3136)
                     /locus_tag="PLE88_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77433.1"
                     /translation="MMRKINAAVMTLAGLLLIAAAGLKFHEMLTICVPSWQSNKLGFW
                     ESYEFLLIQIPLELALGVWMVCGLFRKAAWIAGTLAYLGFIAVTLVKALTGAASCGCF
                     GQIHVNPWITLFSIDIPFFVLLAVFRPAGAKLLPPPWPNVFYLLAVAVPTLGLMALAA
                     PVMVMFRPACIKADDVQPDMTAPLRLQLHQANQMLAAKDEQLKGLSSQIAALHLQIEQ
                     LRQAAAAQTVPQPVPPDKTGPLTQQPIQSQPDDSQTRPPANSQPARPASQQWEWMPYI
                     VEETVRKELAEGIAVVLMYHYDCPTCAQMVPLYSAYCKQMTEQGNEAFKIAFLAVPPY
                     GQGPVPADTTCIQGTLTDQQKWELTSPYVVALLDGQIVKTWPQGTAPAPDKILDDIFG
                     R"
     gene            complement(3126..>3347)
                     /locus_tag="PLE88_13050"
     CDS             complement(3126..>3347)
                     /locus_tag="PLE88_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77434.1"
                     /translation="LGESAWSAGCWEESAAAGNISRQTQEIWEKQPAIGAVSGIFCRQ
                     QASKQQETVPISDNFGYNTARCERTFYDA"
BASE COUNT          758 a         1002 c          922 g          665 t
ORIGIN      
        1 ggccgagaag gaaaagcagt gccagcttct ggccgcggag ttgcagtcca cccgccggcg
       61 ggtcaatgtg ctggagcatg tggtcatccc cgagaccaag gaaaccatca agtacatcta
      121 cgacaagctc ggcgaggccg aacgcgacaa catcagccgc ctgatgaaaa tcgccgacat
      181 tatccggcgc tgacagcact gcggccggct gcttaaaacc gcctgatcgc cagcgtgtgc
      241 accgtcgcat aagccgcccg ccggaacaga tgcagcatca accgcccccg gcgcagcgaa
      301 cctctcttgc gtttcacatc gcgggtcagc catttgttgt tgcccaccgt cacaagggca
      361 tagggggtaa tccacgacag accgaaaatc cagaacatcg catagagaat cgcccacagg
      421 gcctcgctgc tgtactggcg ccgccacgca taaaacgccg ccggcaccgt gccggtcagg
      481 caggccccca gcagggtgtg gctgatgaac aaagccggat gccacaggat gcagccggtc
      541 agccccgcca gcagaatctg cggaacaatc atctggatga cgctgagcac aaaattcacc
      601 cgtgccccca gcgccggggt ctgccggaag cgggtaaaaa tgaatccggc cattgcaatc
      661 gtttcccgca cattgctgcg ggcccagcgg aggaacatct tgcacagccc tttgtaccgc
      721 accggcacag tggtgtaaac gacggcattg gactgaaaca ccacatgcaa gccctgctgg
      781 agaatggcgt tggtcatggc acggtcttcg ccgatgccgg ccggccggcc cagaaaggtc
      841 tggttgagcc atgcatccag aaccttcatc accgctgtcc ggcggtaagc cgacagagcc
      901 cccggcgtgc agaagacgct gttgacagcg ctttggctgg cgcggacaaa ctcaaagctg
      961 tatgcaaaga cgacttccag catccgggga atgatgtctt tgtgccggtt gagcacggcc
     1021 acattgccgg ccacgccgcc gattttttca tcggcggcaa agggactgac aaggcagcgg
     1081 agcgtatcgg gattgatcag cgagtcgctg tcaatcgtca ccagtacctc agcccggctg
     1141 cggatgaagc cctcgtacag cgcgcggcgc ttgccgccgt ttttggccag acgcaccgct
     1201 tcaatccggc cgccggactg ggcggcggcc tttttcatcc actcccatgt atcgtccgcg
     1261 ctgccgtcat cgacagcgat gatttgaagc ttgtcggcag ggtaatcgct ttgcagcacg
     1321 ctttcgagcg ttttgaagac catgctgcct tcattgtagg ccggcacaac gactgtgcag
     1381 gtaggcagct gcgaatcaga gcacggttcg accggacggt agcggaagaa caaaatcacg
     1441 cgccacagaa acgcacttaa attgatcacc agcagcacct gccccagcag aatatagacc
     1501 tcgccgtagg gctgtgcacg cagaaaatcc accgccatcg caaactggtc ggactgtgtc
     1561 catgcggtta tcgatgaaat taaaaacgcc acaacaatca gagttttcgt tcccatacct
     1621 tttcttcaac gccttttcta aatagtcccc ttccgaatag acattattcc cgcaactgcg
     1681 gatacacccg catagggaaa tggagttcag ggaggaggta aaaaaacaac tataccaatt
     1741 tttatataaa atatcaataa aaatttgttc gcctgcaata agcagccagc agaaaacgtt
     1801 ttaataagtt tatagttgtc tggacattcc taaaaaatcc tcgatttcag cctaaaaaat
     1861 gggcattttt gaaatacggc ataacaggat atatatttgc tttaatgtgt taaggcgggc
     1921 tggcttccgc agattgtaca gtttttttct gctgtcagcg gccgaagatg tcatccaaaa
     1981 ttttatccgg agccggtgcg gttccctgcg gccatgtctt gacaatctgc ccatccagca
     2041 gcgccaccac atagggactg gtcagctccc atttctgctg gtcggtcagc gtgccctgaa
     2101 tgcaggtcgt atcggccgga acggggcctt gaccgtatgg cggcacggcc agaaaagcaa
     2161 tcttgaacgc ctcattgccc tgttcagtca tctgcttgca gtaggcgctg taaagcggaa
     2221 ccatctgggc acaggtcggg cagtcgtagt gatacattaa aaccaccgca atgccctcgg
     2281 caagctcctt ccggaccgtc tcctcgacaa tatagggcat ccactcccac tgctggcttg
     2341 ccggccgtgc gggctggctg ttggccggcg ggcgggtctg gctgtcatcc ggctggctct
     2401 ggatgggctg ctgggtcagc ggtcctgtct tgtccggcgg caccggctgc ggaactgtct
     2461 gtgcggcggc cgcctgccgg agctgctcaa tctgcagatg gagtgcggca atctggctgc
     2521 tgaggccttt gagctgctcg tctttggccg ccagcatctg gtttgcctga tgcagctgca
     2581 gccgcagcgg agcggtcatg tccggctgga catcgtcggc cttgatgcag gcaggtcgga
     2641 acatcaccat caccggcgcc gccagcgcca tcagccccag cgtcgggacg gcgaccgcca
     2701 gcagataaaa cacattcggc cacggcggcg gcagcagctt ggcgcctgcg ggccggaaaa
     2761 ccgccagcag gacaaaaaag ggaatatcga tggaaaacag cgtaatccac ggatttacat
     2821 gaatctgtcc gaagcagccg cacgatgccg cgcccgtcag cgccttgacc agcgtgacgg
     2881 cgataaagcc cagataagcc agcgtgccgg cgatccatgc ggccttgcgg aacagcccgc
     2941 agaccatcca tacccccagt gccaattcca gcgggatctg tatcagcagg aattcgtaag
     3001 actcccaaaa gcccagcttg ttggactgcc acgacggcac gcagatggtc agcatctcgt
     3061 gaaacttcaa tccggccgcc gcaatcagca gcaggccggc cagcgtcatt accgcagcat
     3121 tgattttacg catcataaaa agtcctttcg catcgggctg tattataccc gaaattgtcc
     3181 gaaattggaa ctgtttcctg ctgtttagac gcttgctgcc ggcaaaatat gcctgatacg
     3241 gccccgattg ccggctgttt ttcccaaatt tcctgtgttt gccggctgat gttgccggcg
     3301 gcagcagatt cttcccagca gccggcagac caagccgact cgcccaa
//
LOCUS       DAOTBO010000201         3230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_23562_length_3230_cov_3.512126, whole genome shotgun sequence.
ACCESSION   DAOTBO010000201 DAOTBO010000000
VERSION     DAOTBO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23562_length_3230_cov_3.512126"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2952
                     /locus_tag="PLE88_13055"
     CDS             <1..2952
                     /locus_tag="PLE88_13055"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOM77435.1"
                     /translation="MTFGDLFLDVIGKFNIVEITASSQKEESEGMAKAFDGSIATKWF
                     TGDSYGPPFWIQVKSRDAVVIDGYTITSGNDMPDRDPLNWTLFGSNDGTSWTQIDSRS
                     GVDFPERRQTLSFSFSNATAWQYYKFNFQNNAGNTLQLSEITFHEKTPDIVVENYRRE
                     LDLNEGMARIGYEIDGVKYTREYFISCPDNVLVMRLTSIPGGHLTFRVRVACSHSSPA
                     ITARNGRITLSGSLTSNDMAFESQTQVMQTGGTLTTEADSVSIDSADTVMIIMAMGTD
                     YINQYPNYKGTHPHAAVTARVNAASQRTCNQLRARHLADYQELFNRVVLDLKSARPQT
                     PTDELLAGYHGNNPALETLYFQFGRYLLISSSRPGTLPANLQGIWNNVTNPPWNADYH
                     TNINLQMNYWPAEVTNLSECHLPLLEYIDSLRPRGRQTALLHYGAGGWTTHHENNIYG
                     HTGPSTSYAFYCPGAAAWLCQHLWEHYAFTKDQNYLSAKAYPIMKEACEFWVDYLITD
                     PDDGKLVSSPSYSPENRGLSVGCSMDQQIAWDLLTNTIEAGRILDIDADFRQTLINTK
                     NHLDPGLRIGSWGQLQEWKPDWDSPADTHRHVSHLFALHPGRQISPLTTPQYAAAAKI
                     SLLARGDGGTGWSKAWKINFWARLHDGNHAYLMLREQLDFSTLSNLFDTHPPFQIDGN
                     FGGTAGVAEMLLQSHAGVIHLLPALPDAWPAGSVTGLCARGGYEVEIQWNQGRLQEAK
                     LKARFTDTCSVKSAHLADTPDIAVFEEARQISHTRTGDLITFDVQAGKTYRIRLNPLL
                     PGRMEAERYTSMKGVHTEPTDDEGGGENVGWIDAGDWMEYEPRIVFGGEYTLSCRVAS
                     EMNPIVFDLLQGDVLLTSVNTPTTLGWQTWKTVTRSILLEPSEEPLRISAVSGGWNIN
                     WMHFDLIRPNADLNGNGRVDLPDFAFIADQWLNSDCRHAGWCNHGDISTDGVVDLQDV
                     RIIADFWMNET"
BASE COUNT          775 a          840 c          852 g          763 t
ORIGIN      
        1 atgaccttcg gcgatttgtt tctggatgtc atcggcaaat tcaatattgt cgagatcaca
       61 gccagcagcc agaaagagga aagcgaggga atggccaagg cgttcgatgg ttcgatcgca
      121 accaagtggt ttaccggcga ttcttatggc cctccgttct ggattcaggt caaatcccgt
      181 gacgccgtgg tgatagatgg ctacacgatt acctccggca atgacatgcc tgatcgcgat
      241 cctcttaact ggaccctgtt cggttccaat gacggaacga gctggaccca gattgacagc
      301 cgttccggcg tggatttccc ggaacggaga cagacactga gtttttcttt ttccaacgca
      361 accgcctggc agtactacaa atttaatttt cagaataacg ccgggaatac cctccagttg
      421 tccgaaatta cgtttcatga aaagacgccg gatatcgtcg tggaaaatta ccgtcgggaa
      481 ctggatctca atgaaggcat ggcgaggatt ggctatgaaa ttgacggcgt caaatatacg
      541 cgggaatact ttataagctg tccggataat gtcctggtca tgcggctgac aagcattccg
      601 ggcggacatc tgacatttcg ggtacgagtt gcctgttcac acagcagtcc tgcgataacc
      661 gcccgaaacg gcaggatcac gctcagcggt tccctgacaa gcaatgatat ggcttttgaa
      721 tctcagacgc aggtcatgca aacgggcgga actctgacaa cagaagccga ttctgtcagc
      781 attgattcag ctgatacggt aatgattatc atggctatgg gaaccgatta tatcaatcaa
      841 tatccgaact acaaaggcac ccatcctcac gctgccgtca cggcccgcgt gaacgctgcc
      901 tcccagagga cctgcaatca gcttcgtgca agacatttgg ccgattacca ggagcttttt
      961 aaccgcgttg tgctggacct gaaaagcgct aggccgcaaa ctcccaccga tgaactgctg
     1021 gccggctatc acgggaataa tcctgccctg gaaactcttt acttccagtt cggtcggtat
     1081 ctccttatca gttcctctcg gccgggaacc ctgcctgcca atcttcaggg aatctggaac
     1141 aacgtgacca atcctccctg gaatgcggac tatcatacca atattaacct gcaaatgaat
     1201 tattggcctg cagaagtgac caacctcagc gaatgtcatt tacccttgct tgaatatatc
     1261 gacagtctcc gtccgagggg acgccaaact gctctcctgc attacggagc aggggggtgg
     1321 acgacccatc acgaaaacaa tatttacggg catacaggtc ccagtaccag ttatgcgttt
     1381 tattgccctg gtgctgcggc ctggctgtgt cagcatctct gggaacatta tgcgtttact
     1441 aaggaccaga attatctgtc cgccaaggcc tatccgatca tgaaagaggc ctgtgagttt
     1501 tgggtcgatt atctaatcac cgatccggat gatggaaaac tggtttcctc cccgtcttac
     1561 tctccggaaa accgcggtct ttctgtcggc tgctccatgg atcagcagat cgcctgggac
     1621 ctgctgacca ataccattga ggccggtcgg attcttgata tcgacgctga cttccgtcag
     1681 actctcatca ataccaaaaa tcatctggat cccggtctgc ggatcggcag ttgggggcag
     1741 ttgcaggaat ggaaacctga ctgggacagt cccgcagata ctcatagaca tgtttcgcac
     1801 ctgttcgcgc tccatcccgg ccgccagatt tcaccgctga cgacgccgca atatgccgct
     1861 gccgctaaaa tctccctctt ggcccgtgga gacggcggaa ccggctggag caaggcctgg
     1921 aagattaact tctgggcgcg cctccacgac ggcaatcacg cttatctcat gctccgggag
     1981 cagctggatt tcagcacttt gtccaacctc tttgatacgc atcctccgtt ccagattgac
     2041 ggaaacttcg gcggaaccgc cggtgtggcg gaaatgctgc tccagagtca tgccggtgta
     2101 attcatcttc tgcccgctct gccggacgca tggcccgcgg gcagcgtaac cgggctgtgc
     2161 gcacgaggcg gatatgaagt tgaaatccaa tggaatcagg gccgcctgca ggaagccaag
     2221 ctcaaagccc ggtttacaga tacctgctct gtcaaatccg ctcatctggc agatacgccg
     2281 gatatagcgg tcttcgaaga ggcccggcag atttcgcata cccgaacagg agaccttatt
     2341 acctttgatg ttcaggcagg caaaacatac agaatccgcc tcaatcctct tctgccgggc
     2401 cgtatggagg cggaacggta tacgtccatg aagggtgtgc acaccgaacc gaccgacgac
     2461 gaaggcggcg gtgagaacgt cggctggatt gatgccgggg actggatgga gtacgaaccg
     2521 cgtattgtct tcggcggcga gtatacccta agttgtcgtg tagcttcgga aatgaatcca
     2581 attgtctttg atctgttgca gggcgatgtt ctcctgacca gcgtcaatac accgaccact
     2641 ctgggctggc agacatggaa aaccgtcacc cgcagcattc tgttagaacc atctgaagag
     2701 ccgcttcgaa tcagcgccgt cagcggcgga tggaacatca actggatgca ctttgacctg
     2761 attcgcccga atgcggattt aaatggcaat ggccgagtgg atctgccgga ttttgctttc
     2821 atagcggatc aatggctcaa ttcggattgt cggcatgccg gctggtgcaa tcacggcgat
     2881 atcagcaccg acggggttgt cgatcttcag gatgtccgga taatcgctga cttctggatg
     2941 aacgaaacgt gatgctcgaa tcctggcggg ggaactgtgg cgtcgaacaa caactttgat
     3001 tctgcggatt ttaaaatgaa ggatccctta atccttataa ggttcaacga tgaaaggatt
     3061 cagtatgaag aaaattctca tttttgggac aatattgggt acggcgtttt gttttggaca
     3121 ggttcaattt gagggagaat ttgccccgca tgaaggaatg gtgaaatctc cggaagcgga
     3181 tttcaggcag gaaatttgct tgaacggata ctgggatttt caggggatag
//
LOCUS       DAOTBO010000202         3187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_23946_length_3187_cov_4.947637, whole genome shotgun sequence.
ACCESSION   DAOTBO010000202 DAOTBO010000000
VERSION     DAOTBO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23946_length_3187_cov_4.947637"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /locus_tag="PLE88_13060"
     CDS             complement(<1..2059)
                     /locus_tag="PLE88_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOM77436.1"
                     /translation="MRPTMRRISQAFFPVLFVSFAVFGVGRAEVIWSDEFDGETIDSS
                     IWTYDVGGGGWGNGQLEYNTARRENSYIENGNLVIEARREFYQEATTNQFTSARMLTQ
                     GRFAFKYGTLEARIKLPDVANGLWPAFWLLGNNFDGIPWPECGEIDILEIGSKEGIQQ
                     GLQNRKINAAIHYDNNGTYHFDAGWVDADVDLHLDYHLYKLVWTPTEVTAYVDDVPYW
                     SFDITSDYLREFHQPHFPILNIAVGSWPTGYTGIYDPAGITAPLPAKMYVDWIRLTGH
                     PETELFFGGDIEEDGNFGIFTETTPVNRALTYIEEGQEGPDFNYGNDAVLYIWNNLTE
                     VTPPTPSEGSKCWSFRAAAGTWYGMGVFVPNFRNMKNYSDGILHMDMATTTPETIKVG
                     IKSSVGGESWLFLEAAGEQFGLVRDGDWHHVQIPLNRFSNTDFQTVHQMLMIAGDPPA
                     SAVTLSIDNVWWEPSVPREMPADGSFGVYTETASHKDAGEFVLGADGQFFIWEKTLVD
                     DTENPYEGAESISLKSAAGLDWFGCAFTPNRKYNLTAFRYPESKLCFSLKTSSPASFR
                     IGMKSGNIDGIGQKWISFTAGSDPYGFVRDGQWHVVEIPMSDFMPEVDLSEVSQFFEI
                     LGTSGSISGIEIDDIYFTGGGSPLVGDAIPPSVSITSPPNGAFFSPYEDVAIIAEAGD
                     PDGS"
     gene            complement(2170..2766)
                     /locus_tag="PLE88_13065"
     CDS             complement(2170..2766)
                     /locus_tag="PLE88_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77437.1"
                     /translation="MKKAMIFLCAASLLCSSANAVLLPVTNGGFENGTTGWTLNMLGA
                     GSTMVSSPESPLSGNYSAKFVTDWQGGGGVKAEILQTVTGLSGGTGYDFELWVKGAMG
                     PGGVAWAEIKWFNASHVQVGGTGLINLFAGLSSTTYQQKGGTYITPAGTASGEVSIRL
                     EGGALAAHNVLYVDDVCIPEPASLVLLGVGGFFLRCRK"
BASE COUNT          803 a          927 c          736 g          721 t
ORIGIN      
        1 tgcttccatc aggatcgccg gcctctgcaa taatggccac atcttcatac ggcgaaaaaa
       61 aagccccgtt cggcgggctg gtaatgctga ccgaaggagg aatagcgtcg ccaaccagcg
      121 gactgccgcc tccggtaaag tatatatcat caatttcgat gccggagatc gaaccgctgg
      181 tgccaagaat ttcaaaaaac tgggatactt cggacagatc cacctcgggc ataaaatccg
      241 acatcggaat ttccacaaca tgccactgcc cgtcccggac aaacccgtac ggatcgctgc
      301 ctgccgtgaa ggaaatccat ttttgaccga ttccgtcaat gttgccgctt ttcatcccaa
      361 ttcgaaaact tgccggcgaa ctggttttca gagaaaaaca cagtttgctt tccgggtatc
      421 ggaaggccgt cagattgtat tttcgattcg gcgtaaaggc gcagccaaac cagtccaacc
      481 cggcggcaga tttcagactg atgctctcgg cgccttcgta aggattctca gtgtcatcca
      541 caagagtctt ttcccagatg aaaaactggc catccgcccc caggacaaat tcccccgcat
      601 ccttgtggga agccgtctca gtatagaccc caaaactgcc gtcagccggc atttctctcg
      661 gcacgctggg ttcccaccac acattgtcga tagacagggt taccgccgag gccggcgggt
      721 ccccggcgat catcagcatc tggtggaccg tctgaaaatc ggtgttggaa aatcgattca
      781 gaggaatctg aacgtggtgc cagtcaccat cacgaaccaa tccgaactgc tctcctgcgg
      841 cttccaagaa aagccaggac tcgccgccga cggaactttt gattccgacc ttgatggtct
      901 ccggagtagt cgttgccata tccatgtgga gaatcccgtc ggagtagttt ttcatattgc
      961 ggaaattcgg aacaaacacc cccatgccgt accaggtccc cgcggccgcc cggaaagacc
     1021 agcatttgct tccctccgag ggcgtcggcg gagtaacttc tgtcagattg ttccatatat
     1081 acaaaaccgc atcatttccg taattaaagt cgggcccttc ctgtccttcc tcaatatagg
     1141 tcagagcacg attgaccggc gtagtctccg taaaaattcc gaaattgccg tcttcctcaa
     1201 tatccccccc aaagaacagt tctgtctccg gatggccggt cagacgaatc cagtcaacat
     1261 acattttggc aggcaatgga gctgtgatac cggccggatc atagattccc gtgtacccgg
     1321 taggccagct gccaacggca atattcagga tcggaaaatg gggctgatga aactcccgaa
     1381 gataatccga tgtgatatcg aatgaccagt agggaacatc gtccacgtaa gccgttactt
     1441 ccgtaggagt ccatacaagt ttatagagat ggtaatcgag atgaagatcg acgtccgcat
     1501 caacccatcc cgcgtcaaaa tggtaggttc cgttgttatc gtaatggatc gcagcgttga
     1561 ttttccggtt ctgaagccct tgctggatcc cttctttgga gccgatctcg aggatgtcga
     1621 tctcgccgca ttcaggccag ggaatcccgt caaaattatt tccaagcagc cagaacgccg
     1681 gccataaccc attggcgaca tccggtaatt taatccgggc ttccagtgta ccatacttaa
     1741 aagcaaatcg cccctgcgta agcatgcggg cagaggtaaa ttgattggtt gtcgcttcct
     1801 gatagaattc acgacgcgcc tcaatgacca ggttgccgtt ctcgatatac gaattttccc
     1861 ggcgggccgt gttatattca agctggccgt tgccccatcc tccgccgccg acatcataag
     1921 tccagatgga ggaatcaatc gtctccccgt caaactcgtc cgaccagatt acctccgccc
     1981 ggccgacgcc gaaaactgca aaggaaacaa aaagaacagg gaaaaatgcc tgagaaatac
     2041 ggcgcattgt cggcctcata acggccccct ttcagaaatt ggtgccatct ctattatgcc
     2101 cagactcggg caatcaatcg aaatagacgg cccgtagcca aaccgggccg tctatacgaa
     2161 taaagctgtt tattttctgc agcgaaggaa aaacccgccc acaccaagca gaaccagcga
     2221 agccggctcc ggaatgcata catcatctac atacagcacg ttgtgggcag ccagcgcccc
     2281 gccttcgaga cgaatggaaa cctcgccgct ggccgtgccg gccggcgtaa tataggttcc
     2341 acccttttgc tgataggtcg tactcgaaag acccgcgaaa aggttaatca accccgtgcc
     2401 gccgacctga acgtggctgg cgttaaacca cttgatttca gcccacgcca caccgccagg
     2461 gcccatggcc cctttaaccc aaagttcaaa atcgtaacca gtacccccgg aaaggccggt
     2521 taccgtctgc aggatttcgg ccttgacccc gccgccgccc tgccagtcgg taacaaactt
     2581 tgctgaataa ttaccgctca ggggggattc aggagaactg accatggtgg aacccgctcc
     2641 aagcatgttc agcgtccagc cggttgtgcc gttttcaaaa cctccattgg tgacaggaag
     2701 cagaaccgcg ttagcagaag aacacaaaag acttgccgca cacaagaaaa tcatcgcttt
     2761 tttcattgtt ccgtcctcca aaaaaatgat ctccatttcc ttatatcacc agccgcacgg
     2821 ctgattcgtt tcccccgtac ccccgcaggc cgggggtgaa tgtttttata tatcttttag
     2881 caatcgtttg ctaactgcat atactatagg aaacggccct cccctgtcaa gaaaaatatt
     2941 tttgaatttc agacaatccc cggcccgtct tattttttat gggagcagcc ctctcctccc
     3001 gccgtttctt gttgacggcc cttccctctc tgtctatact gtctcccttt agaaaaaaca
     3061 attttccttt agtcaggaac ccaaataatg agaaaaagta aatcgatgcc gagcgtatcc
     3121 tccagccagc gatgtcattt attcaccagc gaatcggtca ccatgggcca tccggacaaa
     3181 atcgccg
//
LOCUS       DAOTBO010000203         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_24204_length_3157_cov_4.068988, whole genome shotgun sequence.
ACCESSION   DAOTBO010000203 DAOTBO010000000
VERSION     DAOTBO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24204_length_3157_cov_4.068988"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3127
                     /locus_tag="PLE88_13070"
     CDS             <1..3127
                     /locus_tag="PLE88_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOM77438.1"
                     /translation="DRWEAVKIKIPSPWNVNSYMRSDGPDHHDFPTYPADWEKYQMGW
                     LRKTITIPSDWKDSQIILHFEGVAGFAEVFVNGRRVCENFDLFLPFEADITDFAKAGR
                     ETEICVGIRKASLFDDNRTIGRRLIPSGSMWGQHIVGIWQDVYLFALPKIRIQDVYVK
                     PLVSEDLLQLELSIQNDSSQDTEVSISGDVREWKNLAGKDMLTAPAAKWTLGKKAITI
                     PARQAVVPANSSASMTLSVPAGNKLHYWTPESPNLYGLTLFLNALGKQIDAEYQRFGW
                     RQWTFTGNKLCLNGAPIELRGDSWHFQGIPQMTRRYAWAWFTAIKDANGNAVRPHAQV
                     YPRFYLEVADEMGICVLDETSNWASDGGPKFDSDKFWTASDDHLKRLVLRDRNHPSVF
                     GWSLTNENRPIIVHVFNRPDLMPVQIEAWARWVKMCTELDPTRPWISGDGDDDGEGTL
                     PTVVGHYGDENSMRNWSSKGKPWGVGEHSMAYYGTPRQVSKYNGARAYESMLGRMEGL
                     AYECYDLIAMQRKYGASYASVFNIAWYALQPLEVGLQDVSRSPTLQDGIFFTAPYIEG
                     KPGVQPERLGPYSTTFNPGYDPSLPLYKPWPMFEAIKQANAPGGPAPSKWAQIPRPEK
                     KAEFPAPLLYESVLFVGEEASNLKAHLVSRGVQLAQFREGKKCLVIIDGQHSLTDKEA
                     AQIRDLLNQGTDVWIWGIVPKTAAAFNRILPYPVEVTQRQAASFVIKSETPITAGLDH
                     ADFYFCEIQQTPAMEYGLKGPLVENGKVILAACDTDWTRWNKVEESVKTASVLRSERQ
                     TKPAGAALVECAAGNGRILINTMTKFYNTERGGSVLQVMLKNAGVPIKKMEIAPGSGV
                     FDIDGSLTRSLICGAFEAESVQAAYDRDFLEGESTAKPKEGDITQGHKWFVGKTGSEG
                     YFDFNRMNLSGGNSNSAVYMSFWLWSPRPLNNLLIEPDMPKLDLIAGSDDACKIWLNG
                     ELIHEKFGIHPIHVGDIQCKNLPLKQGWNHFLVKVAQGGGQWAFGARLHCTDFPFMAQ
                     LKAAVENPDQK"
BASE COUNT          787 a          715 c          865 g          790 t
ORIGIN      
        1 cgaccgctgg gaggccgtca aaataaagat cccgtctccc tggaatgtca attcttacat
       61 gcggtcagac ggaccggatc accatgattt ccccacctat ccggctgact gggaaaaata
      121 tcagatgggt tggctgcgga aaacaataac catcccctcc gattggaagg acagtcagat
      181 tatccttcat tttgaaggtg tcgctggttt tgcagaagtg tttgtaaatg gccgacgggt
      241 ttgtgaaaac ttcgatctgt ttcttccttt tgaagcggat attacagatt ttgcaaaggc
      301 aggccgggaa accgaaatct gtgtcgggat acgtaaggca tccctgttcg atgataaccg
      361 cacaattgga cgtcggttga tcccttccgg ctcgatgtgg ggtcagcata ttgtcggaat
      421 ctggcaggat gtatatttgt ttgctttacc gaaaatcagg attcaggatg tctatgtcaa
      481 acctctggtg tcagaggatc tcctgcagct ggaattgtcg attcaaaatg acagttcgca
      541 ggacacggag gtgtcgatta gcggggatgt tcgggagtgg aagaatttag cgggaaaaga
      601 tatgctgacg gctcccgccg ccaaatggac gctcggcaaa aaagcgataa caattcccgc
      661 tcgccaagcc gttgtgccgg caaactccag tgcctccatg accctttcgg ttcctgccgg
      721 gaataaactg cattattgga cgcctgaatc gccgaatctc tatggcttaa cattattcct
      781 caatgcattg ggaaaacaga ttgatgctga atatcagcgt tttggctggc gtcagtggac
      841 gtttacgggc aataaactgt gtctgaatgg cgcacccatt gaactgcgcg gtgactcatg
      901 gcactttcag ggcatcccgc agatgacccg ccgctacgcc tgggcctggt ttaccgcgat
      961 caaagacgcc aacggcaatg ccgttcgtcc tcatgcacag gtctatccgc gtttttatct
     1021 ggaagtggcc gatgaaatgg gtatctgcgt tctggacgaa acttccaact gggccagtga
     1081 cggtggtccc aagttcgatt cagataaatt ctggacagcc agtgatgacc atctcaaacg
     1141 actggttttg cgtgatcgaa atcaccccag tgtttttggc tggagtctga ctaacgaaaa
     1201 caggcctatt atcgtgcatg tatttaatcg tcctgacttg atgccggttc agatagaggc
     1261 ctgggcgcgt tgggtaaaaa tgtgtaccga attggatccg acacgtccat ggatctccgg
     1321 tgatggcgat gatgatgggg aaggtacact gccaacggtt gttggacact atggtgatga
     1381 aaacagcatg agaaactggt catcaaaagg aaaaccatgg ggtgtgggtg aacattcgat
     1441 ggcttactac ggtacaccca ggcaggtttc aaaatataat ggggcccgtg cctatgagtc
     1501 catgctgggg cggatggaag gactggctta tgaatgctac gatttgattg ccatgcagcg
     1561 aaaatatggt gcgtcgtatg ccagtgtttt taatattgcc tggtatgcac tccagccgct
     1621 ggaggtggga ttgcaggatg tatcccgctc tcccacgctt caggacggca tattcttcac
     1681 cgcgccttat atagaaggta agcccggtgt gcagccggaa cggcttgggc cttactcaac
     1741 cacattcaat cccggctatg acccttcact gccgctgtat aagccatggc cgatgtttga
     1801 ggcgatcaag caggccaatg cccccggagg gccggctccg tcaaaatggg cacaaatccc
     1861 aagaccggaa aagaaggcgg aatttcctgc cccgcttctc tatgaaagtg tgctgtttgt
     1921 cggagaggaa gcatcgaatc tgaaggcgca tctggtctct cgaggggttc aattggctca
     1981 gttccgtgaa gggaagaaat gccttgtgat tattgatgga cagcattctt tgacagacaa
     2041 ggaggcagcc caaatcaggg atctgctgaa tcagggaact gatgtctgga tttggggcat
     2101 cgttccgaaa actgctgcag cttttaaccg catacttccg tatcctgtcg aagtcacaca
     2161 gcgtcaagct gcttcatttg tgattaaatc cgaaacacca attactgccg gcctggatca
     2221 tgccgacttt tatttttgtg agattcagca aacgcctgca atggagtacg ggcttaaggg
     2281 gcctttggtg gaaaacggca aggtgattct tgcggcgtgt gatacagatt ggactcgctg
     2341 gaacaaagtt gaagagtcgg ttaaaaccgc ttctgttctg cgaagtgaac ggcagacaaa
     2401 accggccgga gcggctctgg tcgaatgcgc cgctggaaac ggacgaattc tgatcaatac
     2461 gatgacgaag ttttataata ccgaacgggg cggttcagtt ttgcaggtga tgttaaaaaa
     2521 tgccggggtc cctatcaaaa aaatggaaat cgctcccggc tcaggagttt ttgacataga
     2581 cggcagcctg acccgaagtc ttatttgcgg tgcgtttgaa gcagaaagtg ttcaggcggc
     2641 ttatgatcgg gatttcctgg agggggaaag tacggcaaaa cccaaagaag gggatatcac
     2701 tcaggggcat aagtggttcg taggaaaaac aggttccgaa ggatatttcg attttaaccg
     2761 aatgaatctg tccggcggca attccaattc cgcggtttat atgagtttct ggttgtggag
     2821 tcctcggccg cttaataatc tgttgattga gccggatatg cctaagttgg atctgattgc
     2881 cggttcggat gatgcctgca aaatttggct taacggcgaa ctgattcatg aaaaatttgg
     2941 tatccatcct atccatgttg gggacattca atgcaagaac ctgccgctca agcaggggtg
     3001 gaaccatttt ctggtcaagg ttgctcaggg cggcggtcag tgggcattcg gagcccgact
     3061 tcattgcacc gactttccat ttatggcaca attaaaggcg gccgtggaga atccggatca
     3121 aaaataagca agggcacctc taaaaatttc agtttca
//
LOCUS       DAOTBO010000204         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_24896_length_3086_cov_3.484988, whole genome shotgun sequence.
ACCESSION   DAOTBO010000204 DAOTBO010000000
VERSION     DAOTBO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24896_length_3086_cov_3.484988"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLE88_13075"
     CDS             complement(<1..261)
                     /locus_tag="PLE88_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77439.1"
                     /translation="MAADEKNDVLNAVQSSSSASLDIVRRRRQGVPAEPTLQGSIKPR
                     LQLQKIQPAPDTTLLCRYELKYRIPETKARAIAQYVSAFIHPD"
     gene            complement(524..1012)
                     /locus_tag="PLE88_13080"
     CDS             complement(524..1012)
                     /locus_tag="PLE88_13080"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOM77440.1"
                     /translation="MEETAKTPSQSAVESRYLVMPQHANPFGTAFGGVLMSWIDMVAS
                     MAAQKHCGTEVVTVSIDSLSFEQPIRIGDQVVLRASVNYVSRTSMEVGVKVLRENPIT
                     NESTPATKAYLTFVAIDAAHRPIPAPPLVPQTEEEKRRAANAALRVRARKLLRQQVKT
                     GA"
     gene            1335..1982
                     /locus_tag="PLE88_13085"
     CDS             1335..1982
                     /locus_tag="PLE88_13085"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOM77441.1"
                     /translation="MAVKANKNPAGSGKDDVRDRLLDAAEKLFCLKGFSRTSVRQLTA
                     EARCNLAAVNYHFKSKEHLYVEMFRRQFEAMIRSRLEAIDYVMQLPQADLEGLLRILI
                     EPPMRRIGQDPAHTQVMRLLVREVLNRQIDSEMIARPMRDKLFNRLGQALKQLVPELP
                     AEKLTLIVCSVDGVLLHSFLFMEYYQKIMPNLTLDELIEHLVRFAASAIRGYAKG"
     gene            1988..>3086
                     /locus_tag="PLE88_13090"
     CDS             1988..>3086
                     /locus_tag="PLE88_13090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOM77442.1"
                     /translation="MQRIGNCLLNGLAVLCTLAGCTVGPDYQPPASALPQEWSDPAWM
                     QQGEPNAVLKAWWTVLDDPVLVHLVDQASQGSRTLRQAYERIAESRALRDYAAGRYYP
                     GADFSASYTRSRESANTAFAFPAMLEEHGSYASGFDAAWELDLFGGIRRTVESAQASL
                     EASVENFRDIQASLYAETAAAYIELRTVQMRLQFAMDNIKVQKATLTLTEDRYKAGLA
                     PELDVAQARQNLANTEAQVPSLRMAEIQVLNRLAVLLGQYPQQLDQTLKTPAAIPLPA
                     AEKLPAAVPADLLRRRPDIRRAERQLAAQTAQIGATASQLYPSFSLWGSFSLVAERFS
                     NAGNWSSRSYSYGPSLGWRVFEGGRIRNMVKA"
BASE COUNT          712 a          754 c          835 g          785 t
ORIGIN      
        1 gtccggatgg ataaacgcgc tgacatactg ggcgatggcg cgggccttgg tttccgggat
       61 tcggtatttg agctcgtaac ggcacagcag ggttgtatca ggagccggct gaatcttctg
      121 aagctgtaat cggggcttaa ttgaaccttg caaggtgggc tcggccggaa cgccctgtct
      181 tctgcgtctg actatatcca gagacgcaga agatgatgat tggacagcat ttaagacatc
      241 gtttttctcg tcggcggcca ttaaacctta tttaccccca ttggcaacac aatgcattct
      301 catcatcccg gctttagaca tcgaaaatca cctcggctaa tccgctgccc ttcgatatcc
      361 aatcagactt cttgaagccg tattgaatac acaatcgaca tactcgaatt acaacctatt
      421 ttaatacaat tctaacactt ttgcaatgat tttttggaaa gaagtgcatt acaagatatt
      481 gcatatcttg tatggcttta gaccaggttt gctatgaagg tttttatgct cctgttttta
      541 cctgctgacg taggagtttt cgagccctta ctcgcagagc cgcattagcc gctcggcgtt
      601 tctcttcctc ggtttgaggc accagcggcg gtgctggaat aggcctatga gcggcatcta
      661 tggccacaaa tgtcagatag gcctttgtgg ctggggtgct ctcgttggta atcggatttt
      721 cccgcaggac cttgacgccg acttccattg aggttcggct tacataattt acgcttgccc
      781 gcagcacaac ctgatcgccg atacgaatgg gctgttcaaa cgacagcgaa tcaatgctga
      841 ccgtaaccac ctctgtccca cagtgttttt gggctgccat tgaggccacc atatcaatcc
      901 agctcattaa cactccgccg aaagcagtcc caaagggatt ggcatgctgg ggcattacaa
      961 ggtagcggct ttccacagcg ctctgagaag gtgtttttgc agtttcttcc atttgcatgt
     1021 ctcctatcca gttaaaataa ttatagttaa tcgaaagtca gctgataaaa aagaaaaatt
     1081 ccgaatcgcc cagtcaatgt atttcaacta ttgctaagtt tatatagcag tgaccaggtt
     1141 ggaggaaatt caagttatac cctatcttat ttaagcagaa ccttgccttt ttcgatctat
     1201 gcatctttca atgttttttg caaaaaagac ttattatgtc tgattatttt cacagatttt
     1261 ttcaggaatc tatttgacaa actggcaaaa tctgtgaaac aattgtttga aataaatgtt
     1321 tgagaaaaaa ataaatggca gtgaaagcaa acaaaaatcc tgcaggctcc ggcaaagatg
     1381 acgttcggga tcgtctcttg gatgcggctg aaaagctctt ttgtctgaaa ggcttcagcc
     1441 ggacttcggt gcgccaatta actgccgagg cccgctgcaa cctggccgcc gtcaactacc
     1501 acttcaaaag caaggagcat ttatatgtgg agatgttccg cagacagttt gaagcgatga
     1561 tccgcagccg attggaagcg attgattatg taatgcagtt gcctcaagcg gatttggagg
     1621 ggcttttgag gattctgatt gagccgccga tgcggcgaat cggtcaagat ccggcgcaca
     1681 cgcaggtgat gcgtctgctg gtccgtgagg tgctcaatcg gcagattgat tctgaaatga
     1741 ttgcccgccc gatgagggac aagcttttta atcggctggg gcaggcactt aagcagctgg
     1801 ttcccgaact gccggccgaa aagctgacgt tgattgtctg cagtgttgac ggggtgctgc
     1861 tgcattcctt tttgtttatg gagtattacc aaaaaattat gccgaatttg acgttggatg
     1921 aattgattga gcatctggtg cgttttgccg cttcagcgat acgcggctat gcgaaaggat
     1981 aggtatcatg cagcgaatag ggaattgtct attgaatggg cttgcggtgc tgtgcactct
     2041 ggccgggtgc acggttgggc cggattatca gccgccggca tcggctttgc cccaggagtg
     2101 gtcggatccg gcttggatgc agcaggggga gccgaatgct gtgctgaaag catggtggac
     2161 agttctggac gacccggtgc tggtgcatct ggttgatcag gcctcccaag gcagccggac
     2221 cctgcggcag gcctatgaac gcatcgccga atcacgggcc ctgcgggact atgcagcggg
     2281 aaggtattat cctggagctg atttcagtgc ctcttatacc cgaagccgcg aaagcgccaa
     2341 tacggcgttt gcctttccgg ctatgctgga ggaacatggt tcctatgcca gcggctttga
     2401 tgccgcatgg gagctggatt tatttggagg catccgtcga acagtcgaat cggcacaggc
     2461 ctcgcttgag gcgtccgttg aaaacttccg cgacattcaa gcaagtctgt atgccgagac
     2521 agccgccgcc tatattgaat tgcggacagt ccaaatgcgg cttcaatttg caatggacaa
     2581 tatcaaagtg cagaaagcca cgctgacgct gaccgaagac cgctataaag caggtctggc
     2641 gcccgaactg gatgtggccc aggcacggca gaatctggcc aataccgaag cacaggtgcc
     2701 gtcgctgaga atggccgaaa tacaggtctt aaaccgtctg gcggtgctgc tggggcagta
     2761 cccgcagcag cttgaccaga cgctcaagac gcctgcggca ataccgcttc cggcagcgga
     2821 gaagcttcct gcggctgttc cggccgacct gctgcgccgg cggcccgata ttcgccgcgc
     2881 cgagcggcag ctggcggcgc agaccgctca aattggtgcg acggcctccc agctgtaccc
     2941 ttcattttcg ctgtgggggt ctttctcgct tgttgccgag cggttttcaa atgccggcaa
     3001 ttggtccagc cgaagctaca gctatgggcc gtcgctggga tggcgggttt ttgagggcgg
     3061 gcgcattcgc aatatggtca aggccg
//
LOCUS       DAOTBO010000205         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_25284_length_3047_cov_3.703209, whole genome shotgun sequence.
ACCESSION   DAOTBO010000205 DAOTBO010000000
VERSION     DAOTBO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25284_length_3047_cov_3.703209"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLE88_13095"
     CDS             <1..209
                     /locus_tag="PLE88_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM77443.1"
                     /translation="KKLTGKSFKQFQIDLKLSKADEFFKKHPEMHVKEVARRLGYKDQ
                     YYFSRLYKKYRGCSPSAAQKRFRP"
     gene            435..770
                     /locus_tag="PLE88_13100"
     CDS             435..770
                     /locus_tag="PLE88_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77444.1"
                     /translation="MNKREIVKLALSGHAVPYTPWSFKFTQESLELLIAYYGTNDIES
                     AVDNHIVRLGSDIGFFREIKSNMFQDVFGVVWDRTIDKDIGIVKGQVLKHPTLDNYTF
                     PNPLDSTVF"
     gene            865..1524
                     /locus_tag="PLE88_13105"
     CDS             865..1524
                     /locus_tag="PLE88_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOM77445.1"
                     /translation="MENLMMDFLDHPEFVHQLMSAIADYNITQIRKVLQYDIDAVYFG
                     DDWGQQNGLIIGPSFWREFILPQLKRMYGVVRNAGKYVFIHSCGDVDELFDDLIEAGV
                     HCFNPFQPEVMDVYALLEQYHGRLAFHGGLSIQKTLPFGTMEEVRSESNRLLQAGLKG
                     GLIFSPSHAVEGDTPLANMLAFIYEAQSQPGYDRKGRIKHKIAELIPQTSLQPTNVRK
                     L"
     gene            1704..>3047
                     /locus_tag="PLE88_13110"
     CDS             1704..>3047
                     /locus_tag="PLE88_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOM77446.1"
                     /translation="MTCMKFLPHLLVTAILFLSIYSAADTHPSGISSRWSREKTHAWY
                     AKQPWLCGFNYIPANAISYTEMWMDYSFDPKRIDTELKIAEEVGFNCCRVVFSYVVWE
                     HDPEGFNARFEQFLTICHKRGLKVIPIFFDDCVFGPITDPVYGRQPEIVEGWYANGWT
                     PNPGHSMVRNPASWNQLEKYVTDIMNRHKIDLRILCWDLYNEPTNAGLGDASIPLLEK
                     VFEWARRVNPVQPLTTGWWNNNPKLNKVIFENSDIITFHNYLSADRLYKQIRELQMLG
                     RPVICTEWLNRGLQSVVITCLPIFYHTNVGCMHWGLVNGRTQTDLNWGHRPGDPEPAL
                     WQHDIYRPDYKPYSSEEIDIFKFYLRTAKSLKRSRLLWADPVLPTSELLAQRWKYTFQ
                     DPGEKWADPDYNDSQWKTGEGGFGTAGTAGLAVGSIWQTGQIWLRKEFQLEDASVS"
BASE COUNT          819 a          674 c          755 g          799 t
ORIGIN      
        1 tcaaaaaact gactggaaaa agttttaagc agtttcagat tgatctgaag ctgagcaagg
       61 cagacgaatt tttcaagaaa catcctgaaa tgcacgtcaa agaagtagcc cgtcggctgg
      121 gatacaaaga tcagtattat ttctctcgtt tgtacaaaaa gtatcgcggc tgcagccctt
      181 cagcagctca gaagcggttc aggccataga ccgttttccc ccagaacata acgctgtgta
      241 tcgaatttca aggcaaaaac aggcatagga ccactccagc cttcttttcg gcaatttgtg
      301 ctaaaattgc aaaaactcca tcatatttgc aggtttgtct attgtatgaa gcaggtataa
      361 aactataatg cactttaatg agttttaagt ttattggctc ttttgatgag tcgatcgaaa
      421 ggatgaaacc tgccgtgaat aaaagagaaa tcgtcaaact cgctttgagc ggtcacgccg
      481 ttccttacac accgtggtct tttaagttta cgcaggaatc tctcgagctt ttgattgctt
      541 attatggaac gaatgacatt gaaagcgctg ttgacaatca tatcgtccgt ctcggcagcg
      601 atattggttt tttccgggaa atcaaatcaa acatgttcca ggatgtattt ggtgtggttt
      661 gggaccgtac gatcgacaaa gacatcggca tagtgaaggg ccaggtatta aagcatccca
      721 cgctggataa ttacacgttt cccaatccgc tggattccac ggttttttga cggcatcgag
      781 aaaaaaatat ccgcctttcc ccattgtttt cgcctctatg aactcggttt ttctctgtat
      841 gaacgtgcct ggtcgcttcg cgggatggaa aatctgatga tggattttct tgatcatccg
      901 gagtttgtcc atcaattgat gtccgccatc gcagattaca atattaccca aatcaggaaa
      961 gtacttcaat acgacatcga tgccgtttat ttcggcgatg actgggggca gcagaacggc
     1021 cttatcatag gaccgtcttt ttggagagaa tttatcctgc cgcagctgaa acgcatgtac
     1081 ggcgttgtgc gaaacgccgg aaagtatgtg ttcattcata gctgcggaga tgtggacgaa
     1141 ctgttcgatg acttgatcga agcaggcgtt cactgcttta atccatttca gccggaggtg
     1201 atggatgtgt atgcgctgct ggagcaatat catggacgct tggcttttca cggcgggctt
     1261 tccattcaaa agacattgcc gtttggaacc atggaggagg ttcgatccga atcaaaccgt
     1321 ttattgcagg ccggtctgaa aggcggactg attttttcac cgtcgcatgc ggtggaggga
     1381 gacactccgc tggccaatat gctggcgttt atttacgaag ctcaatcgca gcccggatat
     1441 gaccggaaag gaagaataaa acataagatc gcggaactta ttccgcagac ttcactgcaa
     1501 ccgacaaatg tgcggaagtt atagtttata acagcagcgg gcgttccgct gcaaaacgat
     1561 aaaaagtacc aatgttttgg ctgtgcattg catacaaagc ggtacctcgc actatataga
     1621 cgtcggactg tcagacggtc attgagttgt aaatacaggc aatggcagtt ttaatgaaca
     1681 tgaaaatgaa aggactcaaa gagatgactt gcatgaaatt cttgccgcac ctgttagtca
     1741 cagccatcct atttttatct atctattctg cagcagatac gcatccttcc ggcatatcat
     1801 cccgatggtc cagggaaaaa acgcatgcat ggtatgctaa acagccttgg ctgtgcgggt
     1861 ttaactacat cccggccaat gccatcagtt atacagagat gtggatggat tattcttttg
     1921 accccaagag gattgacacc gaattgaaaa ttgcagaaga agtgggcttt aactgttgca
     1981 gagtggtctt ctcttatgtc gtttgggaac atgatcccga aggattcaac gctcgcttcg
     2041 aacagttcct cactatctgc cacaagcgag gattgaaagt cataccgatc ttttttgacg
     2101 attgcgtatt cggtccgatt accgatcctg tttacggacg acagccggaa atcgtagagg
     2161 gctggtacgc aaacggctgg acccccaacc ccggccacag tatggtcaga aatcccgcct
     2221 cgtggaacca gcttgaaaag tacgtaaccg atattatgaa tcggcacaag atcgatctgc
     2281 gaattctctg ctgggattta tacaatgaac ctacaaacgc agggttgggt gatgcatcga
     2341 ttcctctgct tgaaaaagta tttgaatggg cacggcgggt caacccggta cagccgctga
     2401 ctacggggtg gtggaataat aatccaaaac tgaataaggt gatttttgag aattcagata
     2461 ttattacctt ccataactat ctatccgcag atcggctgta taagcaaatc agagaactcc
     2521 agatgctggg ccgccccgtt atttgtaccg aatggctgaa ccgggggctc cagtcggtcg
     2581 tgattacctg cctgccgata ttctaccata caaatgtggg atgtatgcat tgggggctgg
     2641 ttaacggtag gacacagacc gatctaaact ggggacatcg gccgggcgat ccggaaccgg
     2701 cactatggca gcatgatatc tatcggccgg actacaaacc ttattcgagc gaggagatag
     2761 acatctttaa gttttatctg cgaaccgcga aatctttgaa gcggtcgcgt ctgttgtggg
     2821 ctgatcctgt tttgcccact tcggaactgc tggctcagcg ttggaaatac acatttcaag
     2881 accccggtga aaagtgggcc gatccagact acaacgatag tcaatggaag acaggagagg
     2941 gcggattcgg gaccgctggt acggcaggtt tagcggtcgg ctcaatttgg caaaccggtc
     3001 agatatggct gcggaaagag tttcaattgg aagatgcttc ggtgtcc
//
LOCUS       DAOTBO010000206         2971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_26031_length_2971_cov_5.696159, whole genome shotgun sequence.
ACCESSION   DAOTBO010000206 DAOTBO010000000
VERSION     DAOTBO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26031_length_2971_cov_5.696159"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            538..1236
                     /locus_tag="PLE88_13115"
     CDS             538..1236
                     /locus_tag="PLE88_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77447.1"
                     /translation="MNHSFDVNVACKIGLEKAILIQHIGYWLCRNKANRICHKDGYWW
                     TYTSSEALAEIFPYLNSRSIRRYMTELERDGYIKSAQFKKQNFWHIKYYALTNEGCKL
                     INLPEDFNSISNSSGQTVQTKSGQTDHSNIYNNNIYNNNIREEIVDENSLDIYYTVDD
                     VKECGIPLGLSEEECEAAYHHYNAQGWKRANGLPITNLASLLSTWRAKLHKFKSPQDE
                     EENRIKQFIQSLPE"
     gene            1242..1694
                     /locus_tag="PLE88_13120"
     CDS             1242..1694
                     /locus_tag="PLE88_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77448.1"
                     /translation="MQRAEFNELVNSMAKIWPKWEKSLEEIELFWKIFSPITLNEARA
                     IVQDAAFENEFKPPRSFIRDKINKRPIAKVSGQTEVFIYNPISKYRRELVIVGGSEDN
                     IAEDYINAAKRYLLKWNIADIDVCEFFFDRKLWMERIYGPFSDNKAAG"
     gene            1981..2427
                     /locus_tag="PLE88_13125"
     CDS             1981..2427
                     /locus_tag="PLE88_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77449.1"
                     /translation="MKMMYKIIGMVVIVMLIISQSMFAGQTDKNKPAKTNAHKSAEKI
                     QNKPRPTKIIPARLIGWQTRVSGFGTITEPIFDTAIELYDPNDPYIPGKPSIAKSTFI
                     EDTRKKTRAEKKNETIQNKIYKKNLWIMNRMNYNNRTDQRRKNAND"
     gene            2414..2956
                     /locus_tag="PLE88_13130"
     CDS             2414..2956
                     /locus_tag="PLE88_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77450.1"
                     /translation="MQTIEVFFYRPDIDHRLLDNIIAGWTSLFNRQAVKLGLICSHTE
                     IGFGDNCWTSTMDGGQGVVCRPKQEVLKNPHRWFYFEFDITDNQAIQLRYVLDFKLAN
                     NAGYDKVAILSFFFPVRFGNQDKFICSEFVYRILYRIAQLADDNHPIKKAVRKENEIP
                     SPIRLAYWLKTAGLEVKTLI"
BASE COUNT          989 a          575 c          701 g          706 t
ORIGIN      
        1 aatcgggctg aaaaaggcaa agaaatctac tgccccaggt gtggaacgaa ggggaaggga
       61 gttgatataa aatgaagata atccctgttt cattaaaaga tgcaaacgct tttgttgcga
      121 aataccaccg tcaccataag ccggttcagg ggcataaatt ttctattgga ttggaatatc
      181 acggcgaact tgtaggggtg gctattgtcg gaaggcccgt agcgagacat agggatgacg
      241 gcagaacttt agaggttacc cggctttgca ccaacggggc gaaaaacgcc tgttctaaac
      301 tttatgcagc tgcatggcgg gcttgccgcg caatgggata tacaagattg gggacttaca
      361 ttctggattc cgaatcaggc acttcattga gggcggcagg gtggaaagag atttacaaca
      421 cacggggccg atcttgggat tgccaaagca ggccaagaac agacaagcac ccgattgggc
      481 caaaaacttt atttcaggta gagatatgac ctaccccggc caaataagga gacaattatg
      541 aaccattcat tcgacgttaa tgtagcctgc aaaatcggat tggaaaaagc aattttaatt
      601 cagcacatcg gctattggct ctgcaggaac aaggccaatc gtatctgcca caaggacggg
      661 tactggtgga catatacttc atcggaagct ttggccgaga ttttcccgta cctgaacagc
      721 cggtctatcc gacgatatat gaccgaatta gaacgggacg gatatataaa atcagcccaa
      781 ttcaagaaac aaaacttctg gcatattaag tattatgcat taacaaatga aggctgtaag
      841 ttaataaatt tgccggaaga ttttaattct ataagcaatt cgagcggaca aactgtccaa
      901 actaagagcg gacaaactga ccattcaaat atatataata ataatatata taataataat
      961 ataagggagg aaattgtcga tgaaaattcg cttgatatat attacaccgt agacgatgtc
     1021 aaagaatgcg gtattccgct tgggctctcg gaagaggaat gtgaagcggc gtatcatcac
     1081 tacaacgctc aaggatggaa gcgggcaaac ggattgccga ttacaaattt ggcgtcgctt
     1141 ttatcaacct ggcgggcaaa gttgcataaa ttcaaatcgc cgcaggacga agaggaaaat
     1201 cggattaaac aattcattca atcgttaccg gagtgatgat tatgcagcgg gcagaattca
     1261 acgaattggt gaactcaatg gccaaaatct ggccaaaatg ggaaaaatcg ctggaggaaa
     1321 ttgaattatt ttggaagatt ttttcgccga taacgctaaa tgaggcacgg gcgatagtgc
     1381 aagatgcggc cttcgaaaat gaattcaaac cgcctcgttc gtttattcgg gacaagatta
     1441 acaaacgacc cattgcaaag gtttccggcc aaacggaagt atttatatat aatccaatca
     1501 gcaaataccg gcgagaatta gtaatcgtcg gcggttcaga agacaatatc gctgaggatt
     1561 atatcaacgc tgccaaacga tatctgttaa agtggaacat cgcagacatt gacgtctgcg
     1621 aattcttttt tgaccgcaaa ttatggatgg agcggattta tgggccgttc agcgataata
     1681 aagcggctgg ataatctctg cggccggatt ataaggatgg tccggaagga acgctgtcaa
     1741 atttgctgcc ggatgggcac ggatgtacac catataatcg gacgggcaaa tttgaattgc
     1801 cggtgggact ttgaaaattt actgttggtc tgccggaagt gccacaacaa tcttcacaat
     1861 cgggctgggt atgcccaaac aatttatcgg cggctcggct gcctggaaaa aattgagaag
     1921 ttaaaacgga aacaattaga aacagtaaga acagaagcgt taaaagaaaa agaaagggaa
     1981 atgaaaatga tgtataaaat tataggaatg gtcgtgatag ttatgttgat tatcagtcaa
     2041 tcaatgtttg ccggacagac agacaagaat aagccggcaa aaacgaatgc ccacaaatcc
     2101 gccgaaaaga tacaaaataa acctcgaccg acaaaaatta ttccggccag attaatcggc
     2161 tggcaaactc gagtatcggg atttgggact attactgaac cgatatttga tacggccata
     2221 gaattatacg accccaatga cccgtatata ccaggcaagc cgagcattgc aaaatcaaca
     2281 ttcattgagg acacaaggaa aaagacacgg gccgaaaaga aaaatgaaac tatccaaaac
     2341 aaaatttaca aaaaaaactt gtggattatg aaccggatga attataataa tcgaacagac
     2401 caaaggagaa aaaatgcaaa cgattgaagt tttcttttac aggccggata tcgaccaccg
     2461 attgttggat aacattattg ccggctggac atccctgttt aatcgccagg ctgtaaaact
     2521 cggcttgata tgctcgcata ccgaaatagg tttcggggac aattgctgga catcaacaat
     2581 ggacggaggt caaggcgttg tctgccggcc aaagcaggag gttcttaaaa atccgcaccg
     2641 ctggttttat tttgaatttg atattaccga taatcaggca atacagcttc gttatgtttt
     2701 ggatttcaag ctggccaata atgccggata tgacaaggtg gcgattttat cgtttttctt
     2761 tccggtacga ttcggcaatc aggataaatt catctgctcg gaatttgtct atcggatatt
     2821 atatcggatt gcccagcttg cagacgacaa tcacccgata aaaaaggcag tccgaaaaga
     2881 aaatgaaata ccaagcccaa tccgtttagc gtactggctg aaaacagccg gattggaagt
     2941 aaaaacttta atttaacgaa atggaaagca a
//
LOCUS       DAOTBO010000207         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_26060_length_2968_cov_5.078956, whole genome shotgun sequence.
ACCESSION   DAOTBO010000207 DAOTBO010000000
VERSION     DAOTBO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26060_length_2968_cov_5.078956"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLE88_13135"
     CDS             complement(<1..492)
                     /locus_tag="PLE88_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77451.1"
                     /translation="MAGQAGSCRKPQPVQLIIAPGILFDVDIPLRNVCFRLVIIIIAD
                     KVLDRIVGKQLDDLISVKIRVIRGFYFVFIRGKKISAQKNRTCLAAGLPGLMLESFEN
                     LQLHAAGADQTRHPLRFAALAAVFLRGLFKVAVPLDIANQAFFFAHLLKPLDHLLYAF
                     AGSR"
     gene            complement(505..1173)
                     /locus_tag="PLE88_13140"
     CDS             complement(505..1173)
                     /locus_tag="PLE88_13140"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HOM77452.1"
                     /translation="MKNRVCTALILSALIGWTCGAVTVDIDQIRDDPNLLAWADRAKE
                     LIEQWHPRLLNMIPTRDFEVPSDIALVFRKGDSGIAWTSDNRITVMSGWIGAHPDDIG
                     LVFHELVHVVQCYKSRLPGWITEGIADYLRWAIYEGKPQSWFPVSNKTNGYRDSYRVA
                     AGFFLWLETDRCPGIVNRINTAARKGDYNDAIFSRLTGASLESLWADYMADRKKEQAP
                     QTGK"
     gene            1560..>2968
                     /gene="uvrB"
                     /locus_tag="PLE88_13145"
     CDS             1560..>2968
                     /gene="uvrB"
                     /locus_tag="PLE88_13145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOM77453.1"
                     /translation="MEFKIHSSFEPRGDQPRAIEALVAGLAAGRRYQTLLGVTGSGKT
                     FTMANVIERVQKPTLVISHNKTLAAQLYEEFKELFPENAVEYFVSYYDYYQPEAYIPQ
                     RDIYIEKDASRNDELDRLRLSATASLTSRRDVIIVASVSCIFGLGSPEDYQASVIGLR
                     AGDTIERNDLLGRFADLQYTRNDLDFARGTFRVRGDVVELWPSYESYAVRIEFFGDQI
                     EALRYIHPVSADVLADESQVFIYPAKHYILPAERIESACASIRTELEERLMQLRSEGK
                     LLEAQRLQARTLYDLEMIQEVGYCSGIENYARHMAGLGPGARPYTLIDYFPEDFLLFI
                     DESHVTIPQLRGMYAGDQSRKQVLVDHGFRLPSALDNRPLRFEEFQQRWKQVIFVSAT
                     PSPYELQLCGGEVVEQIIRPTGLVDPDIIVHPAADQIQHLIGEIQKRIAAKERVLVTT
                     LTKRLAEELSAFLTTKGIR"
BASE COUNT          669 a          780 c          850 g          669 t
ORIGIN      
        1 tcgagaaccg gcgaaagcat acagcagatg gtcaagcggt ttaagaagat gtgcgaaaaa
       61 gaaggcctga ttcgcgatat caagcggaac agctactttg aaaagccctc tgagaaaaac
      121 cgccgcaagc gccgcaaatc gcagcggatg gcgcgtttgg tcagctccag ccgcgtgtaa
      181 ttgcaagttc tcgaaggatt cgagcatcaa cccgggcagg cccgcagcaa ggcatgtccg
      241 gtttttttgc gcgctgattt ttttgccacg aatgaacaca aaatagaaac cacggatgac
      301 gcggattttc acggaaataa ggtcatcgag ctgtttcccg acaatgcggt cgagtacttt
      361 gtcagctatt atgattatta ccagccggaa gcatacattc cgcagcggga tatctacatc
      421 gaaaaggatg cctggcgcaa tgatgagctg gaccggctgc ggctttcggc aactgccagc
      481 ctgaccagcc aacacagatg cggtttattt gccggtttgc ggtgcttgtt ctttctttcg
      541 gtctgccata tagtcggccc acagggattc aagggaagcg cctgtcagcc ggctgaaaat
      601 cgcatcatta tagtcccctt tgcgtgcggc agtgttgatt ctgtttacaa tgcccgggca
      661 gcggtcggtt tccagccata gaaaaaaacc agccgctacg cggtagctgt cacggtagcc
      721 atttgtttta ttgctgacgg gaaaccagct ttggggcttg ccttcataga tggcccagcg
      781 gagataatca gcaatcccct ccgtgatcca gccgggcagg cggcttttgt agcactgaac
      841 cacatgcacc agttcatgga agaccagtcc gatgtcatcg gggtgtgccc caatccagcc
      901 ggacatcact gtaatccggt tatccgaggt ccatgcgatg ccgctgtcgc ccttacggaa
      961 aaccagtgca atatcagagg gaacctcaaa atcccgggtg ggaatcatat tcagcagccg
     1021 cggatgccac tgctcaatca gttccttggc ccgatcagcc catgccagca gattcggatc
     1081 gtccctgatt tggtcgatat ccacagtaac tgccccgcac gtccagccaa tcagagccga
     1141 cagtatcaat gcagtacaca cccgattttt catcttcgat tctcctaagt ttaccgtgcc
     1201 ggacatattt ttttcagtac gcgtattaca caatccgttt tgcaattgtc aaacattatt
     1261 ccggctcagt tgcttttcaa tcgcattagc cggcggccaa aagaaggggg aaatgcattt
     1321 ttaagggcgc ttttgtcgga ttaataagat gtttgaatat gcagccggat gtgataagat
     1381 taggtgctgc cccgggctgg cagaggcgaa aaccactggc ggcttttcaa tccggccgct
     1441 tgcagcccga caaaaaaaac ggccggaggc ggtaaggcaa tggcagcgga attttacaca
     1501 gttgttctgc tgtaggatat tgggatgaaa tcgggcagct tgtgctgccg ggcttttgta
     1561 tggagttcaa gatacacagt tcgtttgagc cgcgtgggga tcagccgcgg gcgattgagg
     1621 cgctggttgc gggtctggcc gcgggcaggc gctatcagac cctgctgggc gttaccggca
     1681 gcggcaagac gtttacaatg gccaacgtca ttgagcgggt gcagaagccg actttggtca
     1741 tttcacacaa caagacgctg gcggcccagc tgtacgagga attcaaggag ctgtttccgg
     1801 aaaatgccgt cgaatatttt gtcagctatt acgattacta ccagccggaa gcatacattc
     1861 cgcagcggga tatctacatc gaaaaggatg cctcgcgcaa tgatgagctg gaccggctgc
     1921 ggctttcggc aacagccagc ctgaccagcc ggcgcgatgt gattatcgtc gccagcgtat
     1981 cgtgtatttt cggcttgggc agtccggaag attatcaggc cagcgtgatc ggcctgcggg
     2041 caggcgatac catcgaacgc aatgacctgc tggggcggtt tgcagatttg cagtacaccc
     2101 gcaacgactt ggattttgcc cgcggcacat ttcgcgtccg cggggatgtg gtagaattgt
     2161 ggccgtcgta tgaatcctat gctgtgcgga ttgaattttt cggcgaccag attgaggcgc
     2221 tgcggtatat tcatcctgtc agcgcggatg ttctggccga cgaatcgcag gtgttcatct
     2281 atccggccaa gcactatatc ctgccggctg agcgcatcga aagcgcctgt gcgagcatcc
     2341 ggacggaact ggaagaacgg ctgatgcaac tgcgcagcga aggcaaactg ctggaagccc
     2401 agcggctgca ggcgcggacc ctgtacgatc tggaaatgat acaggaagtc ggctactgca
     2461 gcggcattga aaactacgcg cggcacatgg ccggtctggg gccgggggca cggccttata
     2521 ccctgattga ctactttccg gaggattttc tgctgtttat tgacgaatcg catgttacca
     2581 ttccccagct tcgggggatg tacgccggcg accaaagccg caagcaggtt ttggttgatc
     2641 acggcttccg gctgcccagc gcgctggata accggccgct gcggtttgag gagtttcagc
     2701 agcggtggaa gcaggtgatt tttgtctcgg ccacgccctc gccgtatgaa ctccagcttt
     2761 gcggcggcga ggtggtcgag cagattatcc gcccgacggg tctggtggat ccggacatta
     2821 ttgtgcatcc ggccgccgac cagattcagc accttatcgg cgagattcaa aagcggatag
     2881 ccgccaagga acgggtgctg gtaaccaccc tgaccaagcg tctggccgaa gagctgtcgg
     2941 ccttcctgac aaccaaaggc atccgctg
//
LOCUS       DAOTBO010000208         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_26070_length_2967_cov_3.799451, whole genome shotgun sequence.
ACCESSION   DAOTBO010000208 DAOTBO010000000
VERSION     DAOTBO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26070_length_2967_cov_3.799451"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..2117)
                     /locus_tag="PLE88_13150"
     CDS             complement(384..2117)
                     /locus_tag="PLE88_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOM77454.1"
                     /translation="MTGRIDYDDFYGLILRVIEGQISDEDFFALEAILQKDRCRRREY
                     VESVLLYAMLHKSNSLFDKQFEGADLCSIKLWEILAEDEKNAPAVAVPKMEPSRPLIQ
                     KVEYKRAQRKFSKSSLLTVLVSAAALLFFIFYAALTAPRQQQPPLGLITQVQEAAAAE
                     HKKPLRTGAAVYPETLTLQKGSMKVELDSGVELVIASPSQIVFETKNSVLLNIGSLVA
                     AIPPQAVSFTVRTPLCSVVDYGTEFGISVDAAGQTQVEVFKGKVDLRDNPNPLVFGVS
                     RFVTAGQIGRFDLSGEITIQAAHEVPAENKNIYVRGLPIEWAAKTRRAVWNDSANWDK
                     GLPNLLHAAFLKDMKPGTACQIDASHSGAARAQAHYINVGLRGYGCLEMSGGQLETNE
                     IWIGRQRGGQGIFTLTGGTVFIGDELESKIVVGGAWEEGGGGQGRLYIRGGSIQFSSD
                     RGSVILGWGAAAEGTIYIESAQLVIPGQLRLGRANDVNGMVVSHEGIGTIYLTNGLLR
                     AGSVVISRGRIEIDGGTLEIGGDVCLQAEQWIRNGQITGTEKNPVTVVYDAQRNLTIV
                     KTAVSDDAVPL"
     gene            complement(2122..2664)
                     /locus_tag="PLE88_13155"
     CDS             complement(2122..2664)
                     /locus_tag="PLE88_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOM77455.1"
                     /translation="MDQKNLDKEQKDEYFLRLLMGNQQNIYAFILAMVHNRDDADDVL
                     QDTITVMWRNFSSYQPGTSFVAWGLTIARNMVLKFYEKNRHSKLKFASEIEEDITRFT
                     AKMLESGESRIEALQNCLKKLNLNDLGLVRLHYEQGKPLKQIAIERGIPVYMIYRMMG
                     HIHKLLAHCIRKTLFPEGAA"
BASE COUNT          692 a          864 c          685 g          726 t
ORIGIN      
        1 tggaagcatt agctccagcc gcttaaaatg ttaataaaat atgcgaaagt ttacactcct
       61 aaaaacaatt tgttatgtca atttcagccc atcagcgggc acactgtgca acaaaactcc
      121 tgttttttct atccactcaa ccaatcctcg ctcataaccg ccaccccttt ctggattttc
      181 ccatccgccg gctgcattcg gccggctttt cccatctccg ctttttatgc tgtacctcat
      241 cgggtacatc gtctgaattc cttgctgctt tctgtccgat tcattttctc atcacccgta
      301 tcttttcaca tagcagtcct ccgtcaatac tcgtttcact ttatgccccc gccgtccggc
      361 aggcggcgtc taatttaacg tatttacagc gggacagcat cgtcggagac tgccgtcttt
      421 acaattgtca gattccgctg cgcatcatac acaaccgtta ccggattctt ttctgtgcct
      481 gttatctgac cgttccgaat ccactgctcg gcctgcaggc acacatcgcc tccgatctcc
      541 agcgtgccgc cgtcgatttc tatccgccct cgggaaataa caacagagcc tgcccgcagc
      601 aggccgtttg tcaaatagat agttccaatc ccttcgtggg aaaccaccat cccattgaca
      661 tcattggccc tgcccagccg caactgtccg ggaatcacca gctgggccga ctcgatataa
      721 atcgttcctt cagcagcagc gccccatccc agaataaccg aaccgcgatc gctggaaaac
      781 tggatgctgc cgccgcggat atagaggcgt ccttgtccgc cgccgccttc ttcccaagcc
      841 ccgccgacga caattttgct ttccagctca tcgccgataa acaccgtgcc tccggtcagc
      901 gtgaagatgc cttgtccgcc ccgctgccgg ccgatccaga tttcattggt ttccagctgt
      961 ccgccgctca tttccagaca gccgtatccg cgaagcccca cattgatata gtgggcctgt
     1021 gcacgggcag ctccggaatg cgaggcatcg atttgacagg ccgtaccggg cttcatatcc
     1081 ttcaaaaaag cggcatggag cagattcggc aatcccttat cccagttggc agaatcgttc
     1141 caaactgccc tgcgggtctt tgcggcccat tcaatcggca ggcctcgaac atagatattc
     1201 ttgttctccg ccggcacctc atgggcggcc tgaatggtaa tttcacccga caggtcaaac
     1261 cggccgattt ggccggctgt aacaaagcgg gaaacgccga acaccagcgg attggggttg
     1321 tcgcggaggt ccactttgcc tttgaaaact tccacctgcg tttgtccggc cgcatccacg
     1381 ctgatgccga actccgtgcc gtaatccacc accgagcaca ggggcgtgcg aacggtaaag
     1441 ctgacggctt gcgggggaat cgccgcgacc agactgccga tattcaataa aaccgaattc
     1501 ttcgtctcga agacaatctg gctgggagaa gcaatcacca attcaacgcc cgagtccaat
     1561 tcgactttca tagacccttt ttgaagggtc aaggtttcag gatagaccgc tgcccctgtc
     1621 ctcagcggct ttttatgttc tgccgctgcg gcctcctgaa cctgcgtaat cagcccgagg
     1681 gggggctgct gctggcgggg ggcggtcaac gctgcataga agataaaaaa cagcagtgct
     1741 gcggcagaca ccagcaccgt cagaagcgaa cttttgctga attttcgctg ggcgcgcttg
     1801 tattcgacct tctgaatcag cggacgggac ggctccattt tgggtaccgc cacggccggg
     1861 gcgtttttct catcctcggc cagaatttcc cacagcttta tactgcacag gtcggctccc
     1921 tcaaactgct tgtcgaacag ggagttggac ttgtgcagca tcgcatacag cagaaccgat
     1981 tcgacatact cccgccgccg gcagcggtct ttctgcagga tcgcctccag cgcaaaaaaa
     2041 tcctcgtcgg aaatctggcc ttcgatgacg cggagaatca atccatagaa gtcgtcgtaa
     2101 tcgatgcgtc cagtcatacg ttcaggcagc tccttcaggg aaaagggttt ttcgtatgca
     2161 gtgggccagc agcttatgga tatgccccat catccgataa atcatataaa ccggtatgcc
     2221 gcgttcgatg gcaatctgct tgaggggctt gccctgctcg taatgcagcc gaaccagccc
     2281 caaatcattg agattgagct tcttaaggca attctgcagg gcctcaatgc gggattcacc
     2341 cgactcgagc atcttggctg taaaccgcgt gatgtcttct tcaatctcgg aagcaaactt
     2401 cagcttggaa tggcggttct tctcatagaa tttcaaaacc atattccgcg caatcgtaag
     2461 cccccatgca acaaaactgg ttcccggctg ataactggaa aaatttctcc acataacagt
     2521 aatagtgtcc tgcagaacgt catcggcatc atcccgattg tgcaccatcg ccagaatgaa
     2581 tgcgtagata ttctgctgat ttcccatcag cagacgcaga aaatactcgt ccttctgttc
     2641 cttatccaga ttcttctggt ccataatagg gccttccaac aacagaagac atacctgcac
     2701 tgacacccat agtaatacga tattctacaa taatttaaca ttttttgaga atattatttt
     2761 aagttttata ctcgatgtgt ccatattcaa aaaaagcggc aatccacccg ttcccgcctg
     2821 cgttgcggcg gcaggcagat aagatgcaag aacttatatt gaaaagattt agggaaggaa
     2881 aaacggtcct tgtccggtgt gaagtatata agaatgggcc gaggtggatt cgaaccaccg
     2941 taggcgttag ccaatggatt tacagtc
//
LOCUS       DAOTBO010000209         2963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_26126_length_2963_cov_3.432944, whole genome shotgun sequence.
ACCESSION   DAOTBO010000209 DAOTBO010000000
VERSION     DAOTBO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26126_length_2963_cov_3.432944"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLE88_13160"
     CDS             <1..713
                     /locus_tag="PLE88_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77456.1"
                     /translation="TGLFAYAADHKGLYPPHPAERPNGSFSVVNYLNYAQHDKSRSKN
                     EGTYYYLGGYLPLVNMFVCPLGPAKDLGELQRQYVNYKTKTAYYTLEGTQTSYNLYWG
                     GYKFTGGLVESGEFVGPTGKGAKEEANLLVSDSVFYWGSVTSYNDFWLSHKPGAGIGT
                     ITSYPNNVDPQFGNYVEVFWIYRLVNKVTGPLPSGIQDLKMNAGYTDGSVDRYNGENT
                     SIVWTEPTNLYYIPRKWR"
     gene            972..1745
                     /locus_tag="PLE88_13165"
     CDS             972..1745
                     /locus_tag="PLE88_13165"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77457.1"
                     /translation="MIKMMLSVLGIFLLASSTQAVLVAQYDYTSSLGADPTTQGWTYL
                     KNATNAFLGGYDASIYYPGQSGWRIVDGTSTGYAYYQITLTDDQTEAMKTGFSVSWTF
                     KLDATAWKNDGTGSVANYYLPPNQSRQGNNGLWIETAGENSFRYYIVATADASGNLAI
                     YDGSVTHVLTSGGGNNGYDTLYTVTVDYDGTNAVLSYGSSTYTLTKQATFGNNRVLFG
                     AHTSGGQGSVVYHAVSFIPEPTTMVLLGLGGLLLRRRQA"
     gene            1944..2903
                     /locus_tag="PLE88_13170"
     CDS             1944..2903
                     /locus_tag="PLE88_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOM77458.1"
                     /translation="MIHFEGLVAAGFTPMDKLGNLRLDIIPAIVEHLIGDGIKGIYVC
                     GSTGEGPSLSGQERKKVAEAYTKAAGGRIPIIVQVGHDSLTEARDLARHAWTIGANAI
                     SAVAPHYYKLDSVDTLVDCLVEIGSAVPQIPLFYYHIPALSGIALPMREFIDAACEHV
                     PNFAGIKYSAATIYDYQDCLDYAQGRCQIFFGCDEMLLSALCVGAQAAIGSTYNFAAP
                     LYHRIINAFKQGDHATARQLQGCSVAMCRLLHDYRGMPAIKTMMKLIGLDCGPTRLPL
                     RALSIEETTRLRQEVEKIGFFDWTGKKLISLGDLQNKLAGSKH"
BASE COUNT          786 a          675 c          777 g          725 t
ORIGIN      
        1 ttacgggatt attcgcttat gccgccgacc acaaaggatt atacccgccc cacccggcag
       61 agcggcccaa cggcagtttc agcgtggtta actacctgaa ttacgcccag catgataaga
      121 gccgttccaa gaatgaaggg acatattatt atttaggcgg ttatcttccg ctggtcaata
      181 tgtttgtctg ccctttggga cctgccaaag atctgggtga attgcagcgg cagtatgtca
      241 attacaaaac caaaacggcc tattataccc ttgagggaac tcaaacctcc tacaatctgt
      301 actggggagg ttataagttt accggaggat tagtagaaag cggggagttt gtaggcccaa
      361 cgggtaaagg cgccaaagag gaagccaatc tgctggtttc cgattctgtt ttttactggg
      421 gttccgtaac cagttacaac gatttttggc tgtcccataa gccgggcgcc gggatcggca
      481 caatcacttc gtatccgaac aatgtcgatc cgcaatttgg aaactatgtc gaggtcttct
      541 ggatttatcg gcttgtcaat aaagtgaccg gccctttgcc gtctggaatt caagatttga
      601 aaatgaatgc cggctacacg gacggcagcg tggaccgcta taacggcgag aatacatcca
      661 ttgtctggac ggaaccgacg aacttgtatt acatcccaag aaaatggcga taagttccgc
      721 cggaacagcg gctgtcaaca gcagcagggg ccaaaaaaca ggccaacttc gtatggttga
      781 ggcggaaggg attccaacga tgtgaacaaa cggtaaatga cggttcattt atcggaggaa
      841 agaaattaac tctttggagg taaggaaaaa tggaaaagtt gtctagtgcg tagaaaacgc
      901 cgcgtgtaaa aggtgggtat ggtacgcagc aattaagaaa ttaagaaaac aattttttgg
      961 aggacgtaaa gatgattaag atgatgctgt cagtattagg tatcttttta cttgcgagtt
     1021 cgacacaagc ggtccttgtt gcccaatatg actataccag cagtttgggg gctgatccga
     1081 cgactcaagg gtggacctat ctaaagaatg ctaccaatgc ttttttaggc ggttatgatg
     1141 cctctatcta ctaccccggt cagagcggct ggcggatcgt tgacggaaca tcaaccggct
     1201 atgcctatta tcagattact cttaccgacg accagactga agccatgaag accggtttct
     1261 ctgtctcatg gaccttcaag ctggatgcaa cggcttggaa gaacgacggc acaggcagtg
     1321 tggcgaacta ctacctgccg cccaaccaaa gccgccaggg caataatggc ttatggattg
     1381 agacggccgg agaaaactct ttccgttatt acatcgtcgc aacagccgat gcatccggca
     1441 acctggcgat ttacgatggc tctgtcaccc acgtgctgac ctcaggcggc ggcaacaacg
     1501 gctatgacac cctctatact gtgaccgtgg actatgatgg cacgaatgcg gttctgtcct
     1561 atggcagcag cacgtatacg ctgaccaagc aggcaacttt cggcaataac cgcgttttgt
     1621 tcggggccca caccagcggc ggacagggaa gcgttgtgta tcatgctgtt tcttttattc
     1681 ctgagccgac gacgatggtt ctgctgggtc tgggcggact gctccttcgc cgccgtcagg
     1741 cataatatgc cctaagagtc taattacacg aatgaccgat atttaaaagt catacggata
     1801 tccggcggcc tgatatgata tgtttcaggc cgccgctatc tgttaaagag agagtatttg
     1861 atacatcgta gcaatttcag acataacaga gggcaaacaa caaaagggtt cagattggcc
     1921 cgaaagaaac agaaaggttg actatgattc attttgaggg attggtcgca gctggcttta
     1981 cacccatgga caaactggga aatctccgtt tagacattat tccggcgatt gttgaacacc
     2041 ttataggcga tggaatcaag ggtatctatg tctgcggcag taccggtgaa ggtccgtctc
     2101 tgtcgggtca agaacgcaaa aaggttgctg aggcctatac taaagcagcc ggcggacgaa
     2161 tcccaattat tgttcaggtg ggtcatgaca gcctgacaga agcaagggat ttggcacgtc
     2221 atgcatggac aatcggggcg aatgctattt ctgcggttgc tccgcactat tacaagctgg
     2281 attccgttga tactctcgtt gactgcctgg tcgagatcgg atcggcagtc cctcagatac
     2341 cgttgtttta ctatcatatt ccggctctgt cgggaatcgc cctcccgatg cgggaattta
     2401 ttgacgcagc atgtgagcat gtgccgaact ttgccggcat caaatacagc gctgctacca
     2461 tttatgacta tcaggattgc ctggattatg cgcagggccg ctgtcagata ttctttggct
     2521 gtgatgaaat gctgctcagc gccctttgtg ttggggcgca ggcagccatc ggcagtactt
     2581 ataatttcgc tgcgccttta taccacagga ttatcaacgc attcaaacaa ggagaccatg
     2641 caacagcacg tcagctgcag ggctgttcag tagcgatgtg caggctgctg cacgattata
     2701 ggggaatgcc tgcaattaaa acaatgatga aactcatcgg tcttgactgc ggaccaactc
     2761 gcttgccgct gcgggcgtta tcaattgaag aaacgactcg tcttcgccaa gaggttgaaa
     2821 aaataggatt ttttgattgg acaggcaaga aactgatttc attgggtgat ttgcagaata
     2881 agctggcagg gagcaagcat taaggtatgt cgaaaatacc gattgaatga attgagtgaa
     2941 acagccaaga tatgggggaa caa
//
LOCUS       DAOTBO010000210         2948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_26293_length_2948_cov_5.740754, whole genome shotgun sequence.
ACCESSION   DAOTBO010000210 DAOTBO010000000
VERSION     DAOTBO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26293_length_2948_cov_5.740754"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..2828
                     /locus_tag="PLE88_13175"
     CDS             84..2828
                     /locus_tag="PLE88_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77459.1"
                     /translation="MQKITNLFLVLLSAATFGYLRADLDGDGQVDLADLAILSEEWLR
                     VDLPQDIEVVGELLPDAGGIYVYGGEYNGRPYYTRTDSEWFIWYLPEVESNVISAVLG
                     GLLDTCYWQSDGDNLFGEYYPHNEAVGIAAVREAISGPEGTTLRLKGRWSVSWLWRPE
                     SSWREVHGVLPIAVLRFLDNSYLSVYYEATYNGETRTGGKFVMTDGTNMVEITANQGW
                     AFADVVKFAVSCGDNEVVLYVETPAEGFLSASNESIAFDNYLTSVQFSANATGEVKGL
                     GAYGECCFWDSALAEVEIQKVFDFDSSAPVPTLQKTADQMLFTRDSIRYNHAMEQLEV
                     NKPMQQNFPAAEQVKDFSVYETVTQLTPIAVIDNKCFYIYGSQLRYTTDNQTYETFVN
                     FAAHPITASSVSEGYTPYSGVLLDDGFIIICFKKVPESRLFRVNPIMGDVEQCIITGE
                     GISDNTMFGSVEKFGWTGVKDNKIVVGEYASALTNANLSKRVYLSEDYGKSFSLIYTR
                     PKQEGQGMHVHLCVFHPTNPNIIYVSFGDSPYSNLIKLTKCETEWAAETTGLRVQPTA
                     ALACGNKLLFGNDGVLPFPTIFLFDTETNTAEAVLRFPLRSTTYPNAPYYTPERDTYC
                     FSIKQLNGVYYASTYMSGGETCYSKGVYASLDGRNWTRLISELITSGFREIVGIDNQN
                     RIWMSYVTSSNWGIYCLPVPKIKNIKFYSAERGITNELSEENSTFKTGKGNWTFSNSY
                     DSSLSGRDSSISFDKIGGSLKVVGKYDGTNNRIQWRVQIPNINAGDFFNLRAYFKPIG
                     IIEKPLEEGESFQSPSLRLGLLQVPSITYQYIQGYLSAYKDGWYIATVWGKAIETYSG
                     GYINISLSLYDLPTERYGEFVVYMDCVQLIKNEDIQYSGTFQPGGTPRADEILIAPAY
                     "
BASE COUNT          803 a          526 c          735 g          884 t
ORIGIN      
        1 aatagtggta acaggggctg ccgggtttat cggctctcat ctgtgtgagc ggctgacggc
       61 aggattgtaa ggagcgtagt ccaatgcaaa aaattacgaa tctgtttctg gtgttgttgt
      121 ctgctgcgac cttcggttat ttacgggcag acctggacgg agatggtcag gttgatttgg
      181 cggatttggc tattctgtct gaagaatggc tgagggttga tcttccgcag gatattgaag
      241 ttgtcggcga gttgctgccg gatgcagggg ggatctatgt ttatggaggg gagtataacg
      301 gccggccgta ttatacacga actgacagcg aatggtttat ctggtatctc ccggaggtgg
      361 agagcaatgt gatctctgct gtgctgggcg gcctgctgga tacttgctat tggcaaagtg
      421 atggggataa tctctttggt gaatattatc cgcacaatga agctgttggc attgctgcgg
      481 taagagaggc aatcagcgga ccggaaggca caacgctgcg gctgaaaggc cgttggtcgg
      541 tgagctggct ttggagaccg gaatcgagct ggcgggaagt gcacggagta cttccgattg
      601 ccgttttgcg gtttttggat aattcgtatt tgtccgtcta ttacgaggcc acttacaacg
      661 gtgaaactcg tacaggcggc aagtttgtaa tgaccgatgg aaccaatatg gttgaaatta
      721 cggccaatca gggatgggct tttgccgatg ttgttaagtt cgccgtttca tgcggcgata
      781 atgaagtggt tctttatgtc gaaacacccg ctgaaggatt tttatctgct tccaatgaat
      841 ccattgcttt tgataattac ttgacttctg tccagttttc tgccaacgca actggcgagg
      901 tgaaagggct tggtgcttat ggagaatgct gcttttggga ttctgcattg gctgaagtag
      961 agattcagaa ggtttttgat ttcgattctt ctgctcctgt tccgaccctg caaaagacgg
     1021 cggaccagat gctgtttacc agagatagta tccgctacaa tcacgcgatg gagcaattgg
     1081 aagtcaacaa accaatgcag cagaactttc ctgcagcaga acaagtaaaa gatttttctg
     1141 tttacgaaac tgtgactcag ttaacgccaa ttgctgttat tgataataaa tgtttttata
     1201 tttacggaag tcaattgcgt tatacaacgg ataatcaaac ttatgaaaca tttgtaaact
     1261 ttgcagctca tcccattact gcttcttccg tttctgaagg atatactcct tatagtgggg
     1321 ttctgttaga tgatggattt attataattt gttttaagaa ggttcccgaa agcagattat
     1381 ttagagtcaa tccaataatg ggagatgtag aacaatgtat aataacaggg gagggtatca
     1441 gtgataatac aatgtttggt tctgttgaaa aatttggctg gacaggagta aaagataata
     1501 agattgtagt tggagaatat gcttctgctt tgacaaatgc taatttatcg aaacgagttt
     1561 atctctctga agattacggc aaatcttttt cattgattta tacccgccca aagcaggaag
     1621 ggcagggtat gcacgttcat ttatgcgttt ttcaccccac gaatccaaac attatttatg
     1681 tctcttttgg tgattcacct tactctaatc tgataaaatt aacaaaatgc gaaacagagt
     1741 gggcagcaga aacaacgggc ttacgagttc agccgacagc ggcattggct tgcgggaata
     1801 aattgctttt tggcaatgat ggagtacttc cttttccaac aatttttctg tttgacacag
     1861 aaacaaatac agcggaagca gtgcttcgat ttccattgcg cagtacaact tatcccaatg
     1921 ctccttatta tacaccagaa agagatactt attgctttag cattaagcaa ctgaatggtg
     1981 tttattatgc gtcaacatat atgagtggtg gtgaaacttg ctattcaaaa ggagtatatg
     2041 cttcgcttga tggaaggaat tggactcgtc taatatcaga acttattact tctggttttc
     2101 gggaaattgt aggtattgat aatcagaata ggatttggat gtcatacgtt accagttcca
     2161 attggggaat ctactgcctg ccagtaccaa agattaaaaa tataaagttt tattccgccg
     2221 aaagaggaat caccaatgaa ttgagcgaag aaaactcaac attcaaaact ggcaaaggga
     2281 attggacatt cagtaattct tatgattctt ctttgagtgg tcgggattct tctatttcat
     2341 tcgacaaaat cggcgggtcg ctgaaagttg taggaaaata tgatgggaca aataatcgca
     2401 tccaatggag agttcaaata cccaatatca atgcgggaga tttttttaat cttcgtgcct
     2461 atttcaagcc gattggaatt atcgaaaagc cgttggaaga aggagaatct tttcaatcgc
     2521 cttctcttag actcggcttg ctgcaagtac catcaattac ttatcaatat attcaagggt
     2581 atctgtctgc ctataaagat ggatggtaca ttgcaacggt ttggggcaaa gcaatcgaaa
     2641 cgtattctgg cggatacatt aacatctctc tgagtctcta tgatttacca acggaacgat
     2701 atggcgagtt tgttgtgtac atggattgtg tgcagttgat aaagaatgag gatattcaat
     2761 attccggcac atttcagccg ggcggtacgc ccagggcgga tgagatattg attgcaccgg
     2821 cgtattaatg attgtattgt gtaccaatgt tacgtgattg aggtgtgaac tgaataaagt
     2881 atgggtaaca ggagctgccg ggtttatcgg ctctcatctg tgtgagcggc tggtgaaagc
     2941 gggctatt
//
LOCUS       DAOTBO010000211         2885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_26991_length_2885_cov_4.142756, whole genome shotgun sequence.
ACCESSION   DAOTBO010000211 DAOTBO010000000
VERSION     DAOTBO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26991_length_2885_cov_4.142756"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLE88_13180"
     CDS             complement(<1..461)
                     /locus_tag="PLE88_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOM77460.1"
                     /translation="MSKVRINQKDIADRLNLSVATVSKALRGDYSDINTETRERVVNM
                     ASQLGYTSTGSRIQQILQDEDENKACMVGVLILRKIHEWQHTNYFAGMSEKCAKMNVS
                     LILHYVSEDTCQLILDKEHQPPAMRDGELSGLILVNHWPEDVAAALAAQMP"
     gene            788..2851
                     /locus_tag="PLE88_13185"
     CDS             788..2851
                     /locus_tag="PLE88_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM77461.1"
                     /translation="MLRRHETHTQLGMKFLLVIGLFMVAFSLFLVYQTWLSSRTALER
                     LLRQQAELAMAFECAIDEVLRSSEGQSAQDAGGDYVPQTIQNIFDTVQKNYPHAILRA
                     GGKELAVLLHTSAGDGSRTYRLFRSNPSLRSVEGPIELNGRKYLAKFQNQPSKTDLED
                     PCAGLQMVAVPLSGYEKMLDEEVLRRFSILMFALLGLLGAIFLTFELLVGRRIKRIAE
                     YFCQAARQEEGFLFAPLRIETQDEIGHLAGSFNQLGQKLAGLYDTLETKVRKRTFKLQ
                     QLNKRLRHKIAECRQAEEQARILAEEAVAANKTKSEFLANMSHELRTPMNAIMGFTEV
                     LSDDTLTAEQRSYVQMISSSSKNLLALINDILDYSKIDAGRMVIEICDCRIGELLTEV
                     ESMLRPLAMQKGIRFEVLQCDMVPEILQTDPLRLRQCLINLIGNAIKFTEAGHVYVNV
                     VCFEEDGQLWVRFDVEDTGIGIPKDKQTVIFDSFTQGDSATTRKYGGTGLGLAITKRL
                     AELMGGSVSVVSQVGAGSVFTLTIPAGVKTPQAVWNKYKQIDEINEMSDTMKGTSMYI
                     GKALVAEDNPSNQKLMQILLQKLGLEVVLADDGVQAVEKGTAGSYDIILMDMQMPNMN
                     GYDATRQLRSQGVKTPIIAVTANAMCGDEQKCLDAGCDGYLSKPIDRTKLNEIIGQFI
                     PAQVS"
BASE COUNT          711 a          697 c          757 g          720 t
ORIGIN      
        1 cagggcattt gggcagctaa agcagcagcc acatcctccg gccagtggtt gactaaaatc
       61 aggcctgaca gttcgccatc ccgcatcgca ggcggctgat gctctttatc taaaatgagc
      121 tggcaggtat cttcgctgac ataatgcagg atgagagaaa cattcatttt ggcgcatttt
      181 tcgctcattc cggcaaaata gttggtgtgc tgccattcat gtatttttcg aaggatgagg
      241 acccccacca tgcacgcttt attttcatcc tcatcctgca gaatctgctg aatccggctg
      301 cctgtgctgg tatagccaag ctggctggcc atattgacta cccgctcacg ggtttccgtg
      361 ttgatgtccg aataatcccc ccgcaacgct ttggaaaccg tcgcaacgga caggttcagc
      421 cggtcagcaa tatctttctg gttgattcgc acttttgaca tactgcccat tttattcttt
      481 ttatcgaaaa aaacaagctt ttttcgaaaa attttcgaaa atctgctaaa aaattttctt
      541 ctccgcagcg aaaactatat actatttttc gaaattacct atcttaaaat gcctttattt
      601 gcatttacaa cactcataat gcgcttcggg gccgtacgag tttttagcaa tctctaatcg
      661 gtaaaagtcc ccaattttaa aaaaatcaca gtttttacat taattgaccc gcttttgggg
      721 tcgatacatc cggcggtgct tacacgacta accgcaatga tggtgttgcc ataatttgga
      781 ttcaagaatg ctgagacgac acgaaacaca tacgcaactg ggaatgaaat ttctgctggt
      841 gatagggctg tttatggtgg ccttttcact gtttttagtc tatcagacat ggctgagcag
      901 ccggacggct ttggagcgtc tgctgcgtca gcaggcggag ctggcgatgg cctttgagtg
      961 tgccattgat gaggtgttaa ggagctccga aggccaatct gcccaagatg ccggcgggga
     1021 ttatgtacct caaactattc agaacatttt cgacacggtt caaaagaact atccccacgc
     1081 cattctgcga gccggcggca aggaactggc ggtgctgctg catacatcag ccggcgatgg
     1141 aagccgcacg taccggctgt ttcgcagcaa tccttcactc cgttcggttg aaggtccgat
     1201 tgagctgaac ggccgaaagt atctggccaa gtttcaaaat caaccgtcaa agaccgattt
     1261 ggaggaccct tgtgccggtc ttcagatggt tgccgttccc ctttcgggtt atgagaaaat
     1321 gctggatgag gaggtgctgc ggcgattttc cattctgatg tttgcgctgc tgggactgct
     1381 gggggccatt tttctgacct ttgagctgct tgtcgggcgg cggattaagc ggattgcaga
     1441 gtacttctgc caagccgccc gtcaggaaga gggttttctg tttgcgccgc tgcggattga
     1501 aactcaggat gaaattggcc atctggccgg tagctttaat caattaggac agaaactggc
     1561 tggtctctat gatacgctgg aaacaaaagt ccgcaagcgc accttcaagc tccagcagct
     1621 caacaagcgg ctgcgccata aaatagccga atgccgtcag gctgaggaac aggcccgcat
     1681 tctggcagag gaagccgtgg ctgccaacaa aaccaagagc gaatttctgg ccaatatgag
     1741 ccatgagctg cggacgccga tgaatgccat catgggcttt actgaggtcc tgtccgatga
     1801 taccctcacc gctgaacagc gttcgtatgt tcagatgatc agctccagca gcaaaaatct
     1861 gctggcattg ataaatgata ttctggacta ttcaaagatt gacgccggcc ggatggtcat
     1921 tgaaatctgt gactgccgca tcggtgaatt gctgacggaa gtggaatcga tgctgcgtcc
     1981 gctggcgatg cagaagggaa tccgctttga ggtgcttcag tgcgatatgg tgccggaaat
     2041 actccagacc gacccgctgc ggctgcgtca gtgtctgatt aatcttatcg gcaatgctat
     2101 caaatttacc gaagccggac acgtgtatgt caatgtcgtc tgcttcgaag aggacggtca
     2161 gctttgggtg cggtttgatg tcgaagacac aggcatcgga atccccaaag acaaacaaac
     2221 ggtaattttt gactcattta cgcagggcga cagcgcgacc acccgcaaat acggcggtac
     2281 ggggctgggg ctggccatta ccaagcggct ggcggagctg atgggtgggt ctgtcagcgt
     2341 tgtcagtcaa gtcggtgccg gctcggtgtt tacattgacc attccggccg gggtcaaaac
     2401 accccaagcc gtctggaaca aatacaagca aattgatgaa ataaacgaga tgtcagatac
     2461 aatgaaagga acgtctatgt atatcggaaa agcattggtt gctgaagaca atccttccaa
     2521 ccagaagctg atgcagatac ttctccaaaa gctggggctg gaggtcgtgc tggcagatga
     2581 cggcgttcag gcggtcgaaa aaggcacggc cggctcgtat gacatcattc tgatggatat
     2641 gcagatgccc aatatgaatg ggtatgatgc cacacgccag cttcgaagtc aaggcgtcaa
     2701 aacaccgatt atcgccgtaa cagccaatgc catgtgcggc gatgagcaga agtgccttga
     2761 tgccggctgc gacgggtact tgtccaaacc gattgaccgc acgaagctta atgaaattat
     2821 cgggcagttt atccccgctc aggtaagctg acgctgtttt gttcgattgc ttcaggaagg
     2881 gcctt
//
LOCUS       DAOTBO010000212         2877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_27072_length_2877_cov_5.937987, whole genome shotgun sequence.
ACCESSION   DAOTBO010000212 DAOTBO010000000
VERSION     DAOTBO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27072_length_2877_cov_5.937987"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2877)
                     /locus_tag="PLE88_13190"
     CDS             complement(<1..>2877)
                     /locus_tag="PLE88_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 98 domain-containing
                     protein"
                     /protein_id="HOM77462.1"
                     /translation="GLPNTAAGQKALADAVRAYTDQDDTPVQSAVKLRRPISPQQPMW
                     LVHIDTWNYADPQKIIALIPQDIRPYVVMNISLSISHDEATSRFQVAEYGYEIAKSWL
                     RTCAENRMWAMVQPSSGGFSHFSDFDLSVYEEFYRDYPNMIGFNYCEQFWGYDSTTDP
                     LSAKWTDRINHFANLLALSHKYGGYLVVSWCGNQWSPNINPIGMLKRIPAFAAACRQY
                     TENYILCEKYTQQSYISDMESLCLGAYLSGYSGQYGIRYDDTGWTDANGEHANFTMAT
                     GIAPHLEHIMLTGMTVIDAPELIWTQCFRELSAGSTTNGYTMRRWDTYPQFINVSVDI
                     FRKILDGTVRVPSRREVIDRTKFVIINDVNSGSIDTIYSSPETLFEGLYRMDGDGNLR
                     YNKTFFKKTGRYPTIPTVYQLDDADAQSFQVKVNRSAYSTRWPTISSKVTEFDSLFAS
                     EYTGDIYAGRHENGWVTYNPYKTGQTASGSIPFKYNTCDRMELTYSQYTGGVIKEYPD
                     RVIFYLNNFDNVLDTGLKTNIIKIYGSSSEPTYSWHDRASHQVSTVTKSWTDGVFTLT
                     VQHNGPLDITVNCAGTATGRLTDYTPAVLAAPAKPLIYTGPLQYEGECFDYKYISGIV
                     KGGYSEPVRNYTGQGYLRFGTNSAAAVRDTVTVPETGTYTLETRYSVVGANITTIDLY
                     VNEVLTASPVFTQTATLSDWAIHKQAIFLNAGPNTIEFRARAAAASSIYFDNIVIVPT
                     VYSGGGIAIQENRIGFLGVDGIIDTTDSGYTGDGYADTSDSLGAGIDWEVHFDSSVTK
                     SFTFQYACPDNRTADLIVDGVPIASNIQFPSTGSWSDWKFVTVYARADAGVSDLRLQS
                     TSGLGLPHIDYIQIVGILPLEAPAAPTGLKAMASAGRIDLTWDASDGTTSYNVKRSIV
                     DGGPYSTIASTTETSYSDTELSELTMYYYVVSAVNSAGQSPDSV"
BASE COUNT          728 a          711 c          703 g          735 t
ORIGIN      
        1 gtaccgagtc gggactctgg ccggcactgt tgacggcgct gactacatag taatacattg
       61 ttaattcaga taactccgta tcggagtagc ttgtttctgt cgtgctggcg atagtcgagt
      121 aaggaccgcc atcgacgatt gaacgcttga cattgtaact cgttgtaccg tcagaagcat
      181 cccatgtcaa gtcaatccgg ccggccgatg ccatggcctt cagcccggtc ggggccgccg
      241 gggcttccag cgggagtatt ccgactattt ggatataatc aatatgcggc agacctaaac
      301 cggaagttga ttgaagccgt aaatctgaca ctcccgcatc cgctcttgca tagaccgtca
      361 caaatttcca gtcagaccat gacccggttg atgggaactg gatattggat gcaataggca
      421 caccatccac aatcagatca gccgtcctgt tgtcgggaca ggcatattga aacgtgaagg
      481 atttagtaac ggatgaatca aaatgcactt cccaatctat cccagcgccg agagaatcac
      541 tggtatcggc ataaccgtcc cccgtatagc cggaatcggt tgtgtcaatg ataccatcca
      601 cgccgagaaa gccgatcctg ttttcctgaa tagcaatacc gccgccagaa taaaccgttg
      661 gaacaataac aatattgtca aaatagatag aacttgccgc tgctgctctt gccctgaatt
      721 caatagtatt ggggcctgcg ttgaggaata tagcctgttt atggatagcc caatcgctga
      781 gtgttgcagt ctgggtaaat acaggcgagg ctgtcagtac ctcgttaaca taaaggtcaa
      841 tcgtagtgat atttgccccg acaacggaat accgggtttc aagcgtgtat gtgcctgttt
      901 ccgggacagt aacggtatct cttacggcgg cggcagaatt ggtcccgaat cgcaggtagc
      961 cctgacctgt atagttgcga acaggttcgg aatatccgcc tttcacaata cccgatatat
     1021 atttgtaatc aaaacactcg ccctcatatt ggagaggtcc tgtgtaaatc aaaggtttgg
     1081 caggtgctgc aagaacggca ggtgtatagt ccgttaaacg gccggttgcc gtaccggcac
     1141 agttaaccgt gatatcaagc ggaccgttat gctggacggt caacgtaaac actccatcgg
     1201 tccagctttt ggtcacagta ctgacctgat gactggctct gtcatgccag gaatacgtcg
     1261 gctctgaaga acttccgtaa attttgataa tattcgtctt cagcccggta tcgagaacat
     1321 tatcaaagtt attcagatag aatattacgc ggtctggata ttctttaatc acacctccgg
     1381 tatattgcga ataggttagc tccatccggt cgcaggtatt gtacttaaag ggaatagagc
     1441 cgctggccgt ctgacccgtc ttatagggat tgtatgtgac ccagccgttt tcatgacggc
     1501 ctgcatagat atcgcctgta tattcggagg cgaacaaact gtcaaactca gtcacttttg
     1561 aagaaatagt aggccagcgg gtcgaatacg ccgatctgtt gactttgacc tggaatgact
     1621 gtgcatcggc atcatccagc tgataaacgg ttggaattgt tggataccgc ccagtcttct
     1681 tgaagaaagt tttgttgtac ctgagattgc cgtctccatc catcctatag agcccttcaa
     1741 acagcgtctc aggcgaactg tatatagtat caatactgcc ggaattgacg tcgttgataa
     1801 tgacgaactt tgtgcggtcg ataacctccc tgcgactggg aacacgaact gtcccatcca
     1861 gaattttgcg gaaaatatca acgctgacat taataaactg tgggtaggta tcccaccgcc
     1921 gcatggtgta gccatttgtt gtcgagcccg cactgagttc cctgaaacac tgtgtccaaa
     1981 tcagctcggg ggcatcaatc acagtcatgc cggtcagcat aatgtgttca aggtgcggtg
     2041 cgatgcctgt cgccatcgtg aagttggcat gttctccgtt ggcatctgtc cagccggtat
     2101 catcataccg gataccatat tgtccgctgt aaccggaaag atatgctccc agacacagac
     2161 tctccatgtc tgaaatatag gattgctgag tatacttttc acacagaatg tagttttccg
     2221 tatattgacg acaggccgcc gcaaacgccg gaatccgttt gagcatgcca atcgggttga
     2281 tgttgggaga ccactgattc ccgcaccagc taactaccaa ataaccgccg tacttatggc
     2341 tcagcgccag cagatttgcg aaatggttta tccgatccgt ccatttggcc gagagcggat
     2401 cggttgtact gtcatatccc cagaactgct cacagtaatt aaaaccgatc atgttcggat
     2461 agtctctgta aaattcttca taaacggata aatcaaaatc agaaaagtga ctgaatccgc
     2521 cgctggatgg ctggaccatc gcccacattc ggttctcggc acaggttcgc agccatgatt
     2581 tggcgatctc ataaccatat tcggcaactt ggaaccgact ggtcgcctca tcatgactga
     2641 tggataatga aatgttcatt accacatacg gccggatgtc ttgcggaatc aaggcgataa
     2701 tcttttgggg gtctgcataa ttccatgtgt caatatgaac cagccacatc ggctgttggg
     2761 gggagatcgg acgtctcaac ttcacggccg attgcacagg tgtatcgtct tggtccgtat
     2821 aggcccttac ggcatctgcc aaggccttct ggccggcagc cgtattggga agaccgc
//
LOCUS       DAOTBO010000213         2845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_27436_length_2845_cov_5.014695, whole genome shotgun sequence.
ACCESSION   DAOTBO010000213 DAOTBO010000000
VERSION     DAOTBO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27436_length_2845_cov_5.014695"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..211)
                     /locus_tag="PLE88_13195"
     tRNA            complement(136..211)
                     /locus_tag="PLE88_13195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Pro,seq:cgg)
     gene            complement(258..2615)
                     /gene="priA"
                     /locus_tag="PLE88_13200"
     CDS             complement(258..2615)
                     /gene="priA"
                     /locus_tag="PLE88_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOM77463.1"
                     /translation="MQTDRHQNLFGPESEDGIRPAGRIIRAALDTGADALFDYILPEH
                     LGPVQPGQRVQVPFGRSNKLQQAFVVRLIDNPDQAALSRRFKLKTVKAVLDDQPLLDS
                     QLMQLAEWMARYYVCPLGQVLAAMVPAAVKKDAGMRRKTAVYLCQPAGGPEMPASAKQ
                     KALAAVLCRQGAVDADSAMDKEALLQAAGCTDAPLKELIRKGIARTVIKEQMHALPAV
                     PAGLTAPSGPIVLNSHQQAALDFIAARIRDNQFSVSLLHGVTGSGKTEVYIRAIEACI
                     AAGRQAIVLLPEIALTAQTVQRFSCRFSRLAVLHSQLSGPQRNAQWRRIRNNEADVVI
                     GARSAVFAPVGNLGLIVVDEEHEASYKQDTVPRYHGRDAAIKRAALANAHCLLGSATP
                     SLETLHNCRTKKHYTLLKLPHRVKNLPMPEMKLVNMLTAFADTEHKKIQLLSPPLKDH
                     LAQVLSRQEQAILLLNRRGYSNFICCPACKHTLTCRNCDVTLTFHKKQQNGGESTTIL
                     GRHLTGGYAVCHYCLSKTLVPQHCPLCGSTMTMIGLGSQRLEEELHRQFPKARVRRID
                     SDAMAGQDYYNILKDFADQKIDILAGTQMLAKGLHFPNVTLVGVISADTALFLPDFRA
                     NERTFQLINQVAGRAGRSEKKGTVYVQTFFPKQPAIVYAVQNNFDAFVQDELSHRQAC
                     HLPPFWRLAIVAMRDMNYDRLKTAADLTKERIENIMASEKLEMSLRGPLEPAVGRIQR
                     HHRLHLVVQASSPAAIQLLFERMRAMSPIRPAVQTQIDIDPIGVL"
BASE COUNT          618 a          843 c          752 g          632 t
ORIGIN      
        1 atgaaatccc tttaccaaac tatccggcgg ccgctggaaa ccccgatgta tcgaagccgc
       61 cgaagtcaaa gctgcggaat tggtcgctcc agcagcgttt gagatttttt tataaacggc
      121 tgatttgaag aagactcgga gagacaggat ttgaacctgc gacctctgcg tcccgaacgc
      181 agcgctctaa gccaagctga gctactctcc gtgaaaaaga gctgtgcaat atacagcaat
      241 cttttatcaa ttgcaagtta taaaacccca attggatcaa tatcaatctg cgtctgtacc
      301 gccggacgaa tcggactcat cgcccgcatc cgctcaaaca gaagctgaat cgccgccggc
      361 gaagacgcct gtacaaccaa atgcagccga tgatgcctct gaatccggcc gacagccggc
      421 tcaagcggac cccgaagcga catttccagc ttttctgaag ccataatgtt ctcaattcgt
      481 tctttcgtta agtccgccgc cgttttcagt cggtcataat tcatatcccg catagcgaca
      541 attgccaaac gccaaaacgg cggcagatgg caggcctgtc tgtgggacag ttcatcctga
      601 acaaatgcat cgaagttatt ttgcacggca tacacaatcg ccggctgttt tgggaaaaag
      661 gtctggacat agactgtccc ctttttctcg cttcgtccgg cccggcctgc cacctgattg
      721 atcagctgaa atgtccgctc attggcccga aagtccggta aaaacagcgc tgtatcggca
      781 ctaatcacgc ctaccagtgt tacatttggg aaatgcagac ccttggccag catctgggtg
      841 ccggccagaa tgtcaatttt ctggtccgca aagtccttga gaatattgta gtagtcttgt
      901 ccggccatcg catcgctgtc gattcgcctg acgcgggctt tggggaattg ccggtgcagt
      961 tcttcttcca gccgctgcga acccagacca atcatcgtca tcgtcgagcc gcacagcgga
     1021 caatgctggg gcaccagcgt tttagacagg cagtaatggc acaccgcata cccgccggtc
     1081 aggtgccgtc caaggatagt tgtgctctcg cccccattct gctgcttttt atggaaagtc
     1141 agcgtaacat cacagttgcg gcaggtcagc gtatgcttgc aggccggaca gcatataaaa
     1201 ttgctgtatc cccggcggtt cagcagcaaa atcgcctgtt cctgccggct aagcacctgc
     1261 gccagatggt ctttaagcgg cgggctgaga agctggattt ttttatgttc ggtatccgca
     1321 aaagccgtca gcatattaac cagcttcatt tccggcatcg gcagattctt tacccggtgg
     1381 ggcagtttca gcagtgtgta gtgctttttg gtccggcagt tgtgcagggt ttccagcgat
     1441 ggcgtggccg agcccagaag acagtgggca ttggccagag ccgcccgctt gatggccgca
     1501 tcccgtccgt gatagcgcgg cacagtgtcc tgcttgtaac tggcttcgtg ctcttcatcc
     1561 accacaataa gccccagatt gccgacagga gcaaaaacgg ccgaacgcgc cccaatgact
     1621 acatcggcct cgttattgcg aatccggcgc cactgggcat tgcgctgcgg accggaaagc
     1681 tgcgaatgca gcaccgccag ccggctgaac cggcagctga accgctggac agtctgggct
     1741 gtcagggcga tttccggcag cagcacgatg gcctgacgtc cggcggcaat gcaggcctca
     1801 atcgcccgga tatacacctc tgttttcccg ctgccggtga ccccgtgcag cagggatacg
     1861 ctgaactgat tatcccgaat ccgcgcggca atgaaatcca gcgccgcctg ctggtggctg
     1921 tttaacacaa tcggtcctga cggggccgtc aatcccgccg gcacagccgg cagggcatgc
     1981 atctgctctt tgataaccgt tcgggcaatt cccttgcgga tgagttcctt cagcggggca
     2041 tctgtacagc cggctgcctg cagaagggcc tctttatcca ttgccgaatc cgcatcgaca
     2101 gcgccctgcc ggcataaaac tgccgcaagg gctttctgct tggcgctggc cggcatctcc
     2161 ggcccgccgg ccggctgaca cagatacacg gccgttttac gccgcattcc cgcatctttc
     2221 ttgacagccg ccggcaccat cgccgccagc acttgcccca gcgggcagac ataatagcgg
     2281 gccatccatt cggcaagctg catcagctgg ctgtccagca gcggctggtc atcaagaact
     2341 gctttgacgg ttttcagctt gaaacgccgg ctcagggctg cttggtcggg attgtcgata
     2401 agacgcacga caaatgcctg ctgaagcttg ttggacctgc cgaagggaac ctgcacccgc
     2461 tgccccggct gaaccggtcc cagatgctcc ggcaggatgt agtcaaacaa ggcgtcggcg
     2521 ccggtgtcca gcgctgcccg aataatccgg ccggccggcc tgatgccatc ttccgattcc
     2581 ggaccgaaca ggttttgatg tctgtctgtc tgcatatccg tttgttcctg tgcaggactt
     2641 ttttaccata aactcccccc ccaaggcgat tgtttttttg aattgaagca ggcagttaaa
     2701 acagaataga aacaacataa tccgcccgct tgcactgtgg gacaaatgga taatgggagt
     2761 ggaataagag aattatggtt atgccgattg cagtaccggc caatcccaac gattgcgccg
     2821 cttaaccccc gcccgattcc agccc
//
LOCUS       DAOTBO010000214         2845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_27440_length_2845_cov_3.457706, whole genome shotgun sequence.
ACCESSION   DAOTBO010000214 DAOTBO010000000
VERSION     DAOTBO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27440_length_2845_cov_3.457706"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLE88_13205"
     CDS             complement(<1..183)
                     /locus_tag="PLE88_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77464.1"
                     /translation="MIKKVVEISSAGTYLSVKLGQLIVRQDGTEKGRIPCEDIGVLLV
                     DSPGVTYTHSVLTTLLA"
     gene            complement(223..>2845)
                     /gene="cas9"
                     /locus_tag="PLE88_13210"
     CDS             complement(223..>2845)
                     /gene="cas9"
                     /locus_tag="PLE88_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOM77465.1"
                     /translation="NQRRGFLSNRKSGKSKEDSVVNKSATGLQQAMLQKQVRTLGELF
                     SKLNPHEQRIRGHYTYRAMYEDEFEQLWQKQAAYNGHLLNDVLKKAIKDETIFYQRPL
                     RWDPDTISNCQLEPNQKCCPRADWHARRFRILQTVNNLKINNPDGTQAELAEPQREIL
                     LKELYSRNEVPINSALKQKLRLLERQLFNVEEKAIADKEVKLKGDEFAAQLKKILGKK
                     DFEKLEDADLIEINDALIDDSIDDADLISRLMDQYGFTEEQAKAVVNISLPQKYAAFS
                     RLAIQKLLPHLEKGLLTHEAIEAVYGQPQAPKDIKVADRLGFPEDLRNPIVNKALWEV
                     RKVVNALIREYGKPDSIVIEMAREVKGSQRERDEIRLQQWKQEQENKRAEEELKKIGI
                     HKPSRDDIIKYKLWEECGHVCPYTGKPISQTALFGDHPEFQVEHILPYSRTLDDSYMN
                     KTLCWVHENINKGDQTPYEYYAEKYPEQFEAILQRIAVLPYPKRRKFWQKEVELDKFI
                     ERQLNDTRYINRKVVEYLKTLGVAVRGSKGQITAELRHQWGLDSILNSELPGLKNRQD
                     HRHHAVDAAVTAVVNQKHLHALAQSKYKRNDAAFAPPWESFRSDLEDRVNRINVSHEV
                     TRKVSGQLHEETNYGLTGLKDDKGQDIYVYRKPLDALTPPMVGKIVDPVVRSIIQARL
                     DEFGIRPGGKGTIPKEVWKEPVYMKSRSGVKVPIKKVRIRDVFNNMIILKDKEGKPYR
                     AVAPGNNHHIEIFEYTDKKGNIKRDGIVVSMFEAVHRSRAGKPVVCRDYGDGRKFVCS
                     LAKNEMFMLEVDGEYILHRVQKIIQDGRIVLRPHTFAGQVSDSDNPPFVQRKNYNTLQ
                     GYKVFVDPLGRIFPAKD"
BASE COUNT          659 a          698 c          608 g          880 t
ORIGIN      
        1 ggctaaaaga gtcgtcagga ccgaatgggt ataggttacg ccggggctgt caaccagcag
       61 aacgccgata tcctcacagg ggatgcgtcc cttttctgtg ccgtcctgcc tgacaataag
      121 ctggcccagc ttgaccgata aatacgtccc tgcggatgat atttcaacga ccttcttaat
      181 catgccgaca gcccttcggg ggctttatcg gcaaaagaaa gattagtctt tagcagggaa
      241 aattctgcct aaaggatcaa caaagacttt atacccttgc aaagtattat aattttttct
      301 ttgaacaaaa gggggattgt ctgaatcact tacttgacca gcaaatgtat gtggtctaag
      361 aacaatcctt ccatcctgaa tgattttttg tactctatga agtatatatt caccgtccac
      421 ttccagcata aacatttcat tttttgcaag cgagcagaca aattttctgc cgtctccata
      481 gtcccgacaa accaccggct tgcccgctcg gctgcgatgt accgcctcaa acatcgaaac
      541 aacgatgcca tcgcgtttga tgtttccttt tttgtcggtg tattcgaaga tttcgatgtg
      601 gtggttattt cctggggcta cggcacgata gggtttacct tccttatctt tgaggattat
      661 catattgtta aaaacatctc ttatccgcac tttcttaata ggtactttga caccgctgcg
      721 gcttttcata taaaccggct ctttccagac ttccttaggt attgttccct taccgccggg
      781 acggatacca aactcatcca atcgcgcttg gatgatagat cgaaccaccg gatcgacaat
      841 cttaccgacc atcggaggag tcaatgcgtc taagggtttt cgataaacat agatatcctg
      901 tcctttatcg tcttttaatc cagtcaggcc atagtttgtt tcttcgtgaa gctgaccgga
      961 taccttccgc gttacttcgt gggatacatt aatacgattg actctgtctt ccaaatcact
     1021 gcggaaactt tcccatggcg gggcaaatgc tgcatcgttc cgcttgtatt tgctttgggc
     1081 taacgcgtgg aggtgttttt gattgaccac tgccgtcacg gccgcatcga cagcgtgatg
     1141 gcgatggtcc tggcgatttt ttaagcccgg cagttcactg ttaaggatgg aatccaaacc
     1201 ccattgatgg cgcagttctg ccgtaatctg tcccttgctg cctcgaaccg cgactcccaa
     1261 ggtttttaga tactccacaa ccttgcggtt gatatagcgg gtgtcattaa gctgacgctc
     1321 aataaattta tccagctcaa cttccttttg ccaaaacttt cggcgtttgg gatacggcag
     1381 aacagcaatc cgctgcagaa tcgcctcaaa ctgctcgggg tatttttctg catagtattc
     1441 gtagggcgtt tggtcgccct tattaatatt ctcgtgtacc cagcaaaggg ttttattcat
     1501 ataactatcg tcaagcgtac gggagtaggg cagaatatgc tccacttgaa attcaggatg
     1561 atccccaaac aaggctgttt ggctgattgg tttaccggta taaggacaaa catgtccgca
     1621 ttcttcccat agtttgtatt taataatatc atcacgggaa ggtttatgaa taccgatttt
     1681 ctttaattct tcctctgcgc gtttattttc ctgttcctgc ttccactgct gcagacggat
     1741 ttcgtcgcgc tctctctggg agcccttgac ctcccgcgcc atttcaataa caatgctatc
     1801 gggtttgccg tattcgcgaa tcagggcatt gacaactttt cggacttccc acaatgcttt
     1861 attcacgatg ggatttctta aatcctcagg aaaaccgagc cgatccgcaa ctttgatatc
     1921 cttcggtgcc tgcggctgcc cgtaaacggc ttcgattgct tcatgcgtca acaggccttt
     1981 ttccaaatgc ggcagcaatt tctgaatggc caatcgagag aaggccgcat atttctgcgg
     2041 caatgaaata ttgacaacgg ctttggcttg ttcttccgta aaaccatatt gatccattaa
     2101 gcggcttatg aggtctgcat catcgatgct gtcatctatg agagcatcgt ttatttctat
     2161 taaatctgca tcttccagct tttcaaaatc ctttttccca agtattttct tcaactgtgc
     2221 agcaaattca tcacctttca gtttgacttc cttatcggcg atggcttttt cttctacatt
     2281 gaataactgc ctttcaagca gtctgagctt ctgcttcaat gcggaattaa tcggaacctc
     2341 attgcgtgaa taaagttctt tcagcaggat ttcacgctgc ggttccgcta attctgcttg
     2401 tgtaccgtcg ggattgttga ttttcagatt gttgacggtt tgtaaaatac gaaatcgacg
     2461 tgcatgccaa tccgcccgcg ggcagcattt ttgattcggt tccagctggc aattgctgat
     2521 ggtatccgga tcccatcgca gcggacgctg gtaaaaaatg gtttcatctt taattgcctt
     2581 cttgagtaca tcattcagca aatgcccgtt gtaagctgcc tgcttttgcc aaagctgctc
     2641 aaattcgtct tcatacattg cccgataggt ataatggccg cggatacgct gttcatgcgg
     2701 gtttagtttt gaaaacaact cgcctaacgt tcgaacctgc ttttgcagca ttgcttgctg
     2761 gagtcctgtt gcggacttgt taacaacact gtcttctttg ctctttccgc ttttgcgatt
     2821 gctcagaaaa ccgcgacgct gattc
//
LOCUS       DAOTBO010000215         2820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_27778_length_2820_cov_3.444846, whole genome shotgun sequence.
ACCESSION   DAOTBO010000215 DAOTBO010000000
VERSION     DAOTBO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27778_length_2820_cov_3.444846"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLE88_13215"
     CDS             complement(<1..927)
                     /locus_tag="PLE88_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOM77466.1"
                     /translation="MKYYRNLRDVDKLSADQCALLEPVVQKYAFRANAYYLSLIDWDN
                     PADPIRRQIIPSLDELDEGGRLDPSSEQTYTILTGLEHKYNSTVLLLVSNVCEGICRY
                     CFRKRVFMKPGQELLRDVDGAMAYIGVHKEITNVLLTGGDPLVLTAGKLEAIISRLRQ
                     IAHVQIIRIGTRVPVFHPDRILEDPELADLISTYSLPAKKIYVMNHFCHPRELTDKAL
                     AACDCLRRAGAVLTNQCPLIRGVNDDPDVLAELMQKLSFAGIVPYYLFQCRPALGNYA
                     YAVPIEEGYQIVEAAKSKVSGLAKRLRYVISHA"
     gene            complement(1141..1677)
                     /locus_tag="PLE88_13220"
     CDS             complement(1141..1677)
                     /locus_tag="PLE88_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOM77467.1"
                     /translation="MAAQQSARQKVIEVLNKARAMELHAIHQYMNQHYNLDDMDYGEL
                     AANLKLIAIDEMRHAEMLAERIKELGGEPTTEKAAKVEREQPVEAIFPFDSKLEDDTI
                     ITYNQFLQVCRESGDSVSMKLFETIVDEEQAHFNYFDNVDDHIKQLGASYLARIAGTP
                     ADTGGPSKGFIAAQGGGA"
     gene            complement(1856..2470)
                     /locus_tag="PLE88_13225"
     CDS             complement(1856..2470)
                     /locus_tag="PLE88_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77468.1"
                     /translation="MRPGVLYGVCLALTALAGGCGFGRARTPWDDFKALQTEKTQMAM
                     QMETLEAENARLRQQAETLAALDKPLRLEALDTLSKIRIGRYTGLYDKNGDKAPDVLA
                     VYVEPLDTAQDYIKAAGTCTVAVWDLNAEGPQACLAKWTLSPAELHTHWGGTVFMAYY
                     RLEFPVAGWLNGRHEELTIKVDFTDYLTGKVFSDQRVIKTALLR"
     gene            complement(2479..>2820)
                     /locus_tag="PLE88_13230"
     CDS             complement(2479..>2820)
                     /locus_tag="PLE88_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOM77469.1"
                     /translation="QAASMAAVLFAGGSRSKRFMLPNSRILMHQPLISGVMEGTATDI
                     EIEAREILRIRKRLYSILSEHTGQETAKIEKDCDRNLWLEAEEAVAYGLADKILQKAP
                     QVSRKDTQKGE"
BASE COUNT          571 a          830 c          780 g          639 t
ORIGIN      
        1 ggcgtgcgat atcacatacc gcagccgttt ggccagcccc gaaaccttcg acttggccgc
       61 ttctactatc tggtagcctt cctcgatggg caccgcatag gcataattgc ccagcgccgg
      121 ccggcactgg aacagataat acggcacaat gccggcaaat gacagcttct gcatcagctc
      181 ggccagcaca tccggatcat cgttgacccc gcgaatcagc gggcactgat tggtcaggac
      241 ggcccccgca cgccgcagac agtcgcaggc ggccaaggcc ttgtccgtca gctcgcgcgg
      301 gtggcagaaa tggttcatca catagatttt tttagccggc agactgtacg tgctgatcag
      361 atccgccagc tcggggtcct cgagaatccg gtcgggatga aagaccggaa cccgcgtgcc
      421 gatgcggata atctgaacgt gcgcaatctg ccgcagccgg gagataatcg cctccagctt
      481 gccggccgtc agcaccagcg ggtcgccgcc ggtcagcagc acattggtga tttccttatg
      541 gaccccgatg taggccattg ccccgtccac atcccgcagc agctcttggc cgggcttcat
      601 aaagacccgc ttgcgaaagc agtagcggca gatgccctcg cagacattgc tgaccagcag
      661 caggaccgtc gagttgtact tgtgctccag accggtcagg attgtatagg tctgctcgct
      721 ggaaggatcc agccggccgc cttcatccag ctcatccagc gatgggataa tctgccggcg
      781 gatggggtcg gccggattat cccagtcaat cagcgacagg taatatgcgt tggcacgaaa
      841 ggcgtacttt tgaacaaccg gttccagcaa agcacattgg tcagcagaca gtttgtcaac
      901 atcgcggaga tttcgatagt atttcatcgt gcggcctctt gtttttgaag gttaacaata
      961 catttacaat acgccggtat cccctcgggt gcaggtgcca ttatacccaa tgccgcagac
     1021 gggagcaaga aattataagt cccttcaatt aaagggctta tgtgaaaata aaacaaaaaa
     1081 tcccgcacag ggggcaatct gtcgggactt tttgctgcaa tttaaggtct aaaacagctt
     1141 ttatgcgccg ccgccttggg ctgcgataaa tcccttcgat ggtccgccgg tgtcggccgg
     1201 cgttccggct atccgtgcca gatacgaagc gcccagctgc ttaatgtggt catcgacatt
     1261 gtcaaaataa ttgaaatgcg cctgctcctc atcgacgatg gtctcaaaca gcttcatgct
     1321 gacgctgtcg ccgctttcgc ggcagacctg cagaaactgg ttgtaggtaa taatggtgtc
     1381 gtcttcgagc ttgctgtcga aggggaagat ggcttcgacc ggctgctcgc gttcgacttt
     1441 ggcagctttt tcggttgtcg gctcgccgcc gagttccttg atgcgttcgg ccagcatctc
     1501 ggcgtgccgc atttcatcga tggcaatcag cttgagattg gccgccagct cgccgtagtc
     1561 catatcatca agattgtaat gctgattcat atattgatga atcgcatgga gttccatcgc
     1621 gcgggctttg ttcaagacct cgataacctt ctgtcgggca gattgctggg cagccataat
     1681 tcatctcctt tttgctgaat tgttttccgt ctggttcgta ccgattagag gcattatata
     1741 gaaagctgcc gcaccgtgca aacagaaacc agccggcagc gcacatttct gcctgcggat
     1801 tgccgcagcg aaaaggccgc caaaacacag cacaagggcc ggcgcagaca gccggctagc
     1861 gcagcagggc ggttttgatg acccgctggt cgctgaagac cttgccggtc agataatcgg
     1921 taaaatcgac cttgatagtc aattcctcat gcctgccgtt cagccagccg gcgacgggaa
     1981 actccagccg gtaataggcc ataaatacag tcccgcccca atgggtgtgc agttcggcag
     2041 gcgacagcgt ccatttggcc agacaggcct gcgggccttc ggcgttcaaa tcccaaaccg
     2101 ctaccgtaca ggtgccggcg gccttgatat aatcctgcgc tgtatccagc ggctccacat
     2161 acaccgccag cacatcgggg gccttgtcgc cgtttttgtc ataaagaccg gtgtaccgtc
     2221 cgatgcggat tttggacaag gtatccagtg cttccagccg cagcggcttg tcgagagcag
     2281 ccagtgtttc ggcctgctgc ctgagccggg cattttcggc ctccagagtt tccatctgca
     2341 tggccatctg ggtcttctcg gtctgcaggg ctttgaagtc gtcccacggc gtccttgccc
     2401 gcccgaagcc gcagccgccg gccagagccg tcagcgccag acacaccccg tatagtacgc
     2461 ccggccgcat cgctggtatt actctccttt ctgtgtgtct ttgcggctga cctgcggggc
     2521 cttctgcaga atcttgtcgg ccagaccgta ggccaccgct tcctcggctt ccagccacag
     2581 gtttcggtcg cagtcttttt caatcttggc tgtttcctga ccggtatgct ccgacaagat
     2641 gctgtacagc cgcttgcgga ttcgcaggat ttcgcgggcc tcgatttcga tgtcggtggc
     2701 ggttccttcc atcacgcccg aaatcagcgg ctgatgcatc aggatgcggc tgttgggcag
     2761 cataaaccgc ttgctgcggc tgccgccggc aaacagcact gcggccatgc tggcggcctg
//
LOCUS       DAOTBO010000216         2787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_28173_length_2787_cov_6.210102, whole genome shotgun sequence.
ACCESSION   DAOTBO010000216 DAOTBO010000000
VERSION     DAOTBO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28173_length_2787_cov_6.210102"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>2787)
                     /locus_tag="PLE88_13235"
     CDS             complement(164..>2787)
                     /locus_tag="PLE88_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOM77470.1"
                     /translation="RISRAQKVIEQTQAQMEADKARLETMTQYYEVEEDRKAVHLTHE
                     GIGAAQEIAGVGSFFTGSNMEWPHMLEQALRAHVAFEKEKDYVVMDGKVIIVDEFTGR
                     LMHGRQWSDGLHQAVEAKENVQIKEETQTLATITLQNFFKLYQQIAGMTGTAATEAEE
                     FMKIYHLEVVTIPTNRPCIRDDRDDLIYKTLREKFNAIVDEIHETSAAGRPVLVGTVS
                     IEKSEALSEALTRRYGIEHEVLNAKQHAREAVIVEKAGQQHTTRTGEIRGNVTIATNM
                     AGRGTDIKLGPGVAKIGGLHIVGTERHEARRIDNQLRGRAGRQGDPGSSVFFLSFDDD
                     LMRIFAPEATVKLLSLIGWEEGMPIQHKRISKGIEKAQKKVEERNFEARKSLLEYDEV
                     MDIQRRYFYGSRRKIIEGRDLKQTIWQMVEDMIDGACRNILASDYPSRCIAEWAHREF
                     GVELDSKRLFNLEVEQIEEMIKELARKNVANDISMALGEYLEDYEDPQTWKIDALCKW
                     AMSSFHVSLSAAKLRQMTADAIEQALIEAACEQVNRKSTAGLAEFLNEDYSIRTFSQW
                     AASKFDIHLDLHELKTLGPDAVRQKLTDSVREKYTRREIEYPVEFAMSMVFGPQGPNV
                     YAFESLAKWAQAKYESTLTAEQLQKMPPQEIYQHLLELSRSWNDGKLLSAVENKLSTE
                     SPESLAAWAKERFGAKLDVQQLRQSGQAKDALLAAGREFLRKELTDLERYVLLQIYDV
                     AWKDHLYAMDHLKESIMLRAYAEKDPKIEYKQEGHRMFNEMLETIEDRVTDIIFRVRL
                     EAGQRARSVYNVSRAQHNQVSQFEMAQRQRAAAQAPQGEQKVKQIKLEGPKVGRNDPC
                     PCGSGKKYKKCCGKNTP"
BASE COUNT          565 a          775 c          680 g          767 t
ORIGIN      
        1 tgtttttgtt gataaaccat taaaattagt cgcctaaaaa gtgcgaaagt ttacatttag
       61 atgcttttgt aaaaaggttc cgcgttagct catctgaaat ccgagtctgt cttatccatg
      121 gatggttaat cagcataaat atgggggccc aaagcagcca agactatggc gtatttttgc
      181 cgcagcattt tttgtatttt ttgccgctgc cgcagggaca cgggtcattg cggccgactt
      241 taggaccttc gagcttgatt tgcttgactt tctgctcgcc ctgcggtgcc tgagcggcag
      301 cccgctggcg ctgagccatt tcaaactggc taacctgatt gtgctgtgcc cggctgacgt
      361 tatagacgct gcgcgctcgc tgaccggcct ctaaccgaac ccggaagatg atgtctgtca
      421 ctcggtcttc gatggtttcg agcatctcat tgaacatacg gtggccttcc tgcttgtact
      481 cgatcttcgg atctttttct gcataagccc gcagcataat gctttcttta aggtgatcca
      541 tcgcatagag atggtctttc catgcgacat cgtaaatctg cagcagcaca taccgctcca
      601 gatcggtcag ctctttgcgg aggaattcgc gccctgcggc cagcaaagcg tctttggcct
      661 gcccggactg ccggagctgc tggacatcga gttttgctcc gaatcgttct ttagcccacg
      721 cggctaaact ttcagggctt tctgtgctaa gcttgttctc cacggctgac agcaatttgc
      781 cgtcgttcca ggaacggctc agttccagca gatgctgata gatctcctga gggggcatct
      841 tctgaagctg ttcggctgtg agcgtcgatt catatttggc ctgcgcccat ttagccagcg
      901 actcaaaggc atagacattg ggtccctgcg ggccgaaaac catgctcatc gcaaattcta
      961 ccggatattc gatctcgcgg cgagtgtact tttcccggac gctgtcggtc agtttctgcc
     1021 gaacagcatc aggccccagc gtcttgagtt cgtgcaggtc cagatggata tcaaacttgg
     1081 aagccgccca ttgagaaaag gtgcgaatcg agtaatcctc attaagaaat tcagccagtc
     1141 cggccgtgct tttgcggttc acctgttcac aggcggcttc aatcaaagcc tgctcaatag
     1201 catcggcagt catctggcga agtttcgctg ctgacagcga tacatgaaag ctgctcattg
     1261 cccatttgca cagcgcatca attttccatg tctggggatc ttcgtaatct tccagatatt
     1321 cacccagtgc catcgagatg tcattggcga cattcttcct ggccagttct ttaatcatct
     1381 cctctatctg ttcgacctca aggttgaaca gacgtttgga atccaattct acgccgaatt
     1441 ccctgtgtgc ccattctgct atgcatcggc ttggataatc gctggccaga atattccggc
     1501 aagcaccgtc aatcatatct tcaaccatct gccagatagt ctgtttcagg tcgcggcctt
     1561 caatgatttt gcggcggctt ccataaaaat agcgccgctg aatatccata acttcatcgt
     1621 attccagcag actcttgcgg gcctcaaaat tgcgttcctc cacttttttc tgcgcctttt
     1681 caatcccttt gctgatccgc ttatgttgaa taggcatacc ttcttcccag ccgatcagcg
     1741 acagcagctt gaccgttgcc tcgggagcaa aaatgcgcat caaatcatca tcaaatgaca
     1801 ggaaaaaaac gctgctgccc ggatctccct gacgcccggc gcggccgcga agctggttgt
     1861 caatccggcg ggcttcatga cgctcggttc ctacaatatg caggccgcca attttggcca
     1921 cgccgggccc gagcttgatg tccgtgccgc ggccggccat attggtggcg atggttacat
     1981 tgccgcggat ttcgccggtg cgcgtcgtgt gctgctggcc tgctttttca acgataactg
     2041 cttcgcgtgc gtgctgtttg gcattgagca cctcgtgttc gatcccatag cgacgggtca
     2101 gtgcctcaga cagcgcctcg cttttttcga tgctgaccgt gccgaccagt accggccggc
     2161 cggcagcgct ggtttcatgg atttcatcga cgattgcatt gaatttttcg cgcagcgttt
     2221 tgtaaatcag gtcgtcacgg tcatcccgaa tgcacggccg gtttgttgga atcgttacca
     2281 cctccagatg gtatattttc ataaattctt cggcttctgt ggcggcggtt cctgtcatcc
     2341 cggcaatctg ctggtacagc ttgaagaaat tctgcagggt aatcgtggcc agtgtctgag
     2401 tttcctcctt gatttgaaca ttttccttgg cctcaacagc ttgatgcaaa ccatccgacc
     2461 actggcgtcc atgcatcagg cggcccgtaa actcatccac gataatcact ttgccgtcca
     2521 tcacgacata gtctttttcc ttctcaaacg ctacatgagc tcgcaaggcc tgctcgagca
     2581 tatgcggcca ttccatatta gagccggtaa aaaaggatcc aacgccggcg atttcctgag
     2641 cagccccgat gccctcgtgc gtcagatgaa cggccttgcg atcctcttca acctcgtaat
     2701 actgggtcat agtttcaagc cgggccttgt ctgcttccat ctgggcctgt gtctgttcga
     2761 taactttctg ggcacggctg atgcgtt
//
LOCUS       DAOTBO010000217         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_28947_length_2726_cov_5.334706, whole genome shotgun sequence.
ACCESSION   DAOTBO010000217 DAOTBO010000000
VERSION     DAOTBO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28947_length_2726_cov_5.334706"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..430)
                     /locus_tag="PLE88_13240"
     CDS             complement(101..430)
                     /locus_tag="PLE88_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77471.1"
                     /translation="MDIIETAQKIEDLIKALSAELERSEGLIAQKAETAAAYDKAAAV
                     QAVRMKEDGLPVGLIDKIVKGKLADLNAQKILAEETLRAHFWRLEVLMAQLNGYQSIN
                     KYLNSTE"
     gene            complement(430..969)
                     /locus_tag="PLE88_13245"
     CDS             complement(430..969)
                     /locus_tag="PLE88_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77472.1"
                     /translation="MNFEAVRNHNGQRLEMYATILEIGKQGTTKNGSPYCQMTIADKN
                     GEKHNVSIFVDTILPSPVLLGKNYIFDLQTYQGKYGIAYSGYIRRPADIPALSAANQP
                     AKNFVEKAFQEDSIVLNQSETIRIRSMAISYAKDLIVANCKDNLFETAENIYSWIIGM
                     PITDDEDKSVEMKDEDIPF"
     gene            complement(959..1162)
                     /locus_tag="PLE88_13250"
     CDS             complement(959..1162)
                     /locus_tag="PLE88_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77473.1"
                     /translation="MEKGKKIMDKKEKLRKILDDIQAAEEMAYLAKRFGLDLEELAIN
                     PCRIRDCDKGKWKHFRKGYRNEL"
     assembly_gap    1331..1430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1431..2246)
                     /locus_tag="PLE88_13255"
     CDS             complement(<1431..2246)
                     /locus_tag="PLE88_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77474.1"
                     /translation="MKITNHYNLPEPIVKRLEADIYEPDLNRIGVTTLIDAPLVRHLR
                     IKHWDELEEDASERLWALLGSAVHYLFDNKPFTANRLTEERLELKHPSGLTIVGYNDD
                     YADGVLTDYKVTSVWSYIFGKTSWDKQLNVLAYLLEQNGFAVKKAQICLLLRDWQLRR
                     AETEADYPKQPIAIVDIPLWSTDQRRAYIDERIKIHQSIPVPECTAEDKWERPTTYAV
                     KKKGHKRALRVFDKFEDAQEFLKNNGDLIETRKGERVRCQSYCPVRQFCPFCEY"
     gene            complement(2243..2461)
                     /locus_tag="PLE88_13260"
     CDS             complement(2243..2461)
                     /locus_tag="PLE88_13260"
                     /inference="COORDINATES: protein motif:HMM:NF025456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM77475.1"
                     /translation="MNKTKLVKKWLLKKKSITTFQAFELFGATRLSAIIYSLRKSGMP
                     IKAESVKIKDRYGHTCIIAKYKLKGVKK"
     gene            complement(2458..>2726)
                     /locus_tag="PLE88_13265"
     CDS             complement(2458..>2726)
                     /locus_tag="PLE88_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOM77476.1"
                     /translation="GWRENTKKERKKIMRQKLEFKLNGKDPFAAKIFNLLQEKKMSIY
                     ELGKAAGIHPSSLCAWLNESQRGHTKSITSIRLAKILKSLGEKI"
BASE COUNT          579 a          638 c          530 g          879 t
ORIGIN      
        1 tccccttcgt tccacacctg gggcagtaga tttctttgcc tttttcagcc cgattgtgca
       61 cgccacattt acatcaaagg aatgattcat aattgtctcc ttattctgta ctgttcagat
      121 atttgtttat tgactggtat ccgttcaatt gggccattag cacttccagt cgccagaagt
      181 gtgcccgaag ggtctcttcg gccaatatct tctgtgcgtt caagtcggcc agcttacctt
      241 taacaatctt gtcaattaag cctaccggca gcccgtcttc tttcatccgg acggcctgga
      301 cggccgccgc cttgtcataa gcagcggccg tctcggcttt ttgggcgata agcccttcag
      361 acctttccaa ttcggccgaa agggctttaa tcaagtcctc tatcttttga gctgtttcaa
      421 tgatatccat cagaatggta tatcttcatc tttcatttca acggatttat cttcgtcatc
      481 agttatcggc atgccaataa tccagctgta tatattttca gcagtttcaa atagattatc
      541 cttgcaattt gcaacaatca agtctttggc ataactgatt gccatcgagc gaatgcggat
      601 tgtctccgac tgattgagaa cgattgaatc ttcctggaat gctttttcaa cgaagttttt
      661 tgcgggctgg ttcgctgccg ataatgcggg aatatccgcc ggccgccgga tgtatccgga
      721 ataagcgatg ccgtacttgc cctgataagt ttgcaggtca aagatgtaat tctttcccag
      781 cagcaccggc gaaggcaaaa tggtatcaac gaatattgaa acattgtgct tttcgccgtt
      841 cttgtcggcg attgtcattt ggcaataggg actcccattc ttggtcgttc cctgtttgcc
      901 gatttcaagg atggtagcat acatttccag ccgttgtccg ttgtgatttc ttactgcctc
      961 aaagttcatt tctgtaccct ttccgaaaat gtttccattt gcccttgtcg caatcccgta
     1021 tgcggcaagg attgattgct aattcttcta aatcaagccc gaagcgcttg gcaaggtaag
     1081 ccatttcttc agcagcttga atgtcatcta atattttcct gagtttttcc tttttgtcca
     1141 ttattttttt tcccttttcc atagtcatcc tctctttctg ttttggggga cattaaatcg
     1201 ttcataatct tcttccggcc ttccgtttct tggtatcgtc cgctgatttt cttctgcctc
     1261 gtcggacttt tctacatatt cgcagaacgg gcaaaattgt cgtacggggc agtaagattg
     1321 acagcgaacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atattcgcag
     1441 aacgggcaaa attgtcgtac ggggcagtaa gattgacagc gaaccctttc ccctttgcgg
     1501 gtctcgataa ggtcgccatt attctttagg aattcttggg cgtcttcaaa tttatcaaac
     1561 acccgcaagg cccgcttatg gcctttcttt tttacagcgt aagttgtcgg ccgctcccat
     1621 ttatcttctg ctgtacattc cggtacgggt atagactggt gtatttttat ccgttcgtct
     1681 atgtatgccc tccgctggtc ggttgaccat aacggtatat caacaatagc aatcggctgt
     1741 tttggataat ccgcttctgt ttctgcccgc cggagctgcc agtctcgaag caataagcaa
     1801 atttgtgcct tttttaccgc aaagccattt tgctcgagta ggtatgccag cacattcaac
     1861 tgtttatccc agctggtctt gccgaatata taagaccaaa cactggtcac cttgtaatcg
     1921 gttaaaacgc cgtctgcgta atcatcgttg taaccgacta ttgtcaggcc cgaagggtgt
     1981 ttgagttcca gtctctcttc ggttagtcga ttggccgtaa acggcttatt atcgaacaag
     2041 taatgtaccg ccgaacccag cagagcccaa agccgttccg atgcgtcctc ttccaattcg
     2101 tcccaatgct ttatccttaa atgccggaca agcggcgcat caatcagagt agtcactccg
     2161 atacggttga ggtctggttc gtaaatgtcg gcttccaacc gtttaacaat tggttctggt
     2221 aaattgtaat gatttgtgat tttcattttt ttactccttt aagtttatat tttgcaatga
     2281 tacaagtgtg cccgtatcgg tctttaatct taacagattc ggcctttatc ggcattccgc
     2341 tcttccgaag cgaataaata atcgccgaca gacgggttgc tccaaataac tcaaatgcct
     2401 gaaatgtcgt aatgctcttt tttttgagca gccatttttt tacaagcttt gttttgttca
     2461 tattttttct cctaacgatt ttaaaatttt tgccagccgg atactggtaa tgctctttgt
     2521 atgccctcgc tggctctcgt ttagccaggc acacaaagaa gacggatgta ttcccgctgc
     2581 tttgcccaat tcgtaaatcg acattttctt ttcctgcagc aaattgaaaa tttttgccgc
     2641 aaacggatct ttcccattca gtttgaattc taatttttgc cgcataattt ttttcctttc
     2701 ttttttagtg ttttcacgcc atccat
//
LOCUS       DAOTBO010000218         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_29134_length_2712_cov_5.508092, whole genome shotgun sequence.
ACCESSION   DAOTBO010000218 DAOTBO010000000
VERSION     DAOTBO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29134_length_2712_cov_5.508092"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..958
                     /locus_tag="PLE88_13270"
     CDS             143..958
                     /locus_tag="PLE88_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HOM77477.1"
                     /translation="MDNNNLYIPTDIFIMEPAEEYHAKANDYLSSHQLIDFMRCPLLH
                     HKKRMGIIQDQETTSFLVGRAAHVRILEGRAEYESQFAMGGPINPTTGKPYGSLTKKF
                     AEWRDEQKKPVLTFEQMELVEQMARGVAINDTAVDLISYGSAEGVIRADYCGVPCQIR
                     LDWLNPNRGIVDFKTCDDLTWFEADARRYRYPNQMAFYQAVLKEVIGVYVPVHLIAIE
                     KKEPHRCGVWRVSDDCLAMARHDNEAAIGRLESCRNKDIWPTGYEEVRLLDVA"
     gene            1150..1887
                     /locus_tag="PLE88_13275"
     CDS             1150..1887
                     /locus_tag="PLE88_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOM77478.1"
                     /translation="MTLLESVLNQNHMRAPKGIVYGPPGVGKTTFGAGTHKPIIIDCE
                     NGAAHMNCDRTPYLADWDAILPWLEALAHDEHTYGTVVIDSIDWLLRRLEERVAGVNG
                     DARNMDKTMNRSHGGYGNGKQVLRNYVYQYLLPTLDAIVNRGISVVLLAHASRRSITT
                     IDGIVMEKSAPEIHPDLMNTLVEWSDFVGAARMDGDCRELVLNETGQLLAKNRYGITG
                     VLPLDWNCLMAAMSNHKPQLTGEIING"
     gene            1880..2338
                     /locus_tag="PLE88_13280"
     CDS             1880..2338
                     /locus_tag="PLE88_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HOM77479.1"
                     /translation="MANLNGFDANQVEPNVGFEPVPAAKYIAMVTASEMKPTKNGDGS
                     YLELELTILEGPFKDRKVWDRLCLNHPNAQTVKIARGYLSAICRATGVMQPKDSCELH
                     NIPMCITVKCKKRDDNDEITNEIRGYAKKESVAGKPQQAAPTDNTPPWKR"
     gene            2335..2637
                     /locus_tag="PLE88_13285"
     CDS             2335..2637
                     /locus_tag="PLE88_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77480.1"
                     /translation="MTMNRKAKGARLERKTIKMLQSAGYQCIRSAASLGPFDIIAMNH
                     LGFRCIQIKANDWPRPEEREGLRAAAIGLPPNALIECWRWNDHAREPIIKLLDEFN"
BASE COUNT          694 a          696 c          739 g          583 t
ORIGIN      
        1 ggtcagaatg tgtcacgtgt tagtcacgtg acggtcacgt gactaaaaat gaccctataa
       61 attcgggatg cgtttttggc ttgaatttcg aataaccctt tagaggcgaa caatggggtt
      121 tgccaaggca acggagacga aaatggacaa caacaatctt tatatcccga cagacatctt
      181 tattatggaa cccgccgagg aatatcacgc caaggccaat gactatctga gcagtcatca
      241 gctgattgat ttcatgagat gcccgctgtt gcatcacaaa aagcgaatgg gcatcatcca
      301 ggaccaggaa accacctctt ttctggtggg ccgtgccgca cacgtgcgga ttctggaagg
      361 ccgagctgaa tatgaaagcc aatttgctat gggcggcccc atcaatccga caaccggcaa
      421 accctatggc agcctgacca agaagttcgc cgaatggaga gacgagcaga aaaagcccgt
      481 tttaacattt gagcagatgg agttggtcga acagatggcc cgcggtgttg ccatcaatga
      541 taccgcggtg gacctgattt cctacggtag cgccgaaggt gttatccgcg ccgactattg
      601 cggcgttccc tgtcagattc ggctggactg gctcaatccg aatcgcggca tcgtggattt
      661 caagacctgc gatgatttga catggtttga ggccgacgca cgtcggtatc gctatcccaa
      721 tcagatggcc ttctatcagg cggttctgaa agaagtcatc ggtgtttatg ttcctgtcca
      781 tctgattgct atcgaaaaga aagaacccca ccgctgtggc gtctggcgcg tatccgacga
      841 ttgcctggcc atggcccggc atgataatga ggcggcgatt ggtcgtctgg aatcatgccg
      901 caacaaggac atctggccga ccggctacga ggaagttcgg ctcctggatg tcgcatagcc
      961 ccccatcttt gcccggacgg tttttgcggt tggtcaccac gaaaacgaca gcccgccgtc
     1021 cgggccattt cggcgaggaa aacggttttg cccgggtctc ataagcccgc ggttatcggt
     1081 tcgactccgg cactcgccat tggacccggc cggtcaaaac aacatgaaac attaatcaga
     1141 ggaataaaca tgacactatt ggaatctgta ctcaatcaaa accacatgcg tgcccccaag
     1201 ggcatcgtgt acgggccacc gggagtgggt aagaccactt tcggtgcagg cacgcacaaa
     1261 ccgatcatta tcgattgtga aaatggcgcg gcacatatga attgcgaccg gacgccgtat
     1321 ctggccgact gggatgccat cctgccatgg ctcgaagctc tggctcatga tgagcatacc
     1381 tatggaaccg tggtcatcga ttcgattgac tggctcctgc ggcggctgga agagcgtgtg
     1441 gccggtgtca atggcgatgc cagaaacatg gacaagacca tgaatcgctc gcatggcggc
     1501 tatggtaacg gaaagcaggt gcttcgcaac tatgtctacc aatacctgct gccaacactg
     1561 gacgccatcg tcaaccgtgg gatttcggta gttctgttgg cccatgcctc tcggcgcagt
     1621 attacgacca tcgacggtat cgtgatggaa aagtccgccc cggaaatcca tccggacctg
     1681 atgaacacct tggtcgagtg gtcggatttt gtcggcgctg cccgaatgga tggtgactgt
     1741 cgggaactgg tgttgaatga aactggtcag ttgctggcca agaaccgcta tgggatcacg
     1801 ggcgttctgc cgttggactg gaactgcctc atggccgcca tgagcaatca caaacctcaa
     1861 ttaactggag aaatcatcaa tggctaatct gaatggattt gatgcaaatc aagttgaacc
     1921 caatgtcggt tttgagccgg ttccggcagc aaagtacatc gcgatggtca ccgcatccga
     1981 gatgaaaccg accaaaaacg gtgacggcag ttatctggaa ctggaattga ccatcctgga
     2041 gggcccgttc aaggaccgca aggtatggga ccggctgtgt ctgaaccatc ccaatgccca
     2101 gaccgtcaaa attgcacgcg ggtatttgtc ggctatctgt cgggctactg gtgtcatgca
     2161 gcccaaggac agctgtgaac tgcacaatat cccaatgtgc atcacggtca agtgcaaaaa
     2221 gcgtgacgac aacgatgaga ttaccaatga aattcggggc tatgccaaaa aggaaagcgt
     2281 tgccgggaaa ccccagcagg cagcgcctac cgataacaca cccccgtgga agcgatgacc
     2341 atgaacagaa aggccaaagg ggcacgtctg gaacgcaaga ccatcaagat gctgcagtcg
     2401 gccggatacc aatgtatccg gtcggcggca tcactgggtc cgttcgacat cattgcgatg
     2461 aatcatctgg gctttcgatg tattcaaatc aaggccaatg actggccccg acccgaagaa
     2521 cgagaaggct tgagggcggc ggcgataggc ctgccgccca atgccctgat tgaatgctgg
     2581 cggtggaatg accatgccag agaacccatt atcaaattac tggacgaatt taactgaatc
     2641 gaaaggatca aacatggaag ggttcctcta aaaattgaca aatttacgtg caattgcttg
     2701 tacctttaaa ta
//
LOCUS       DAOTBO010000219         2709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_29166_length_2709_cov_5.175207, whole genome shotgun sequence.
ACCESSION   DAOTBO010000219 DAOTBO010000000
VERSION     DAOTBO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29166_length_2709_cov_5.175207"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2599)
                     /locus_tag="PLE88_13290"
     CDS             complement(<1..2599)
                     /locus_tag="PLE88_13290"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOM77481.1"
                     /translation="MHRIKLNKAACIRCMQFMLALTVLLPLTAKADIDMVNQSLTYSS
                     LTSTTVNISGVSELHLTSQTPLSGCTINLNSPDAFLFLESVKPSTAVSSYLGQVKVSG
                     AAAVAAFNVRVVEYGIGSVIIPQPPTFAPLQVFSEENFAGSWAYLTQYTTYNTSMLGT
                     MANNISSFILKRGYTATFAQNENGTGFSKNYVAQDCDLEIGVLPGKLDNKINFVRVFP
                     WRWVSKKGIAGNIGQNLDIKWWYNWNIDQNSTLDKEYVPIRQNRWWPGLNQNWQTRGA
                     SHLLGYNEPDHTEQANIAVGDAIWSWPDLLATGLRVGSPATTDGGRSSWLYPFMQRAD
                     AADLRVDFVAVHYYWCYDPANPTGAANQMYNFLKAVYDQTGRPIWVTEWNNGANWTGC
                     ADPTYAQQAAAVAAMINMLDITPFVERYAIYNWVEDCRRVQWDDGWPTQAGEVYRDQI
                     SPIGYQQEVPGSGKSACAVYTFDNHFRDSSGNGNHPLCYGIPARSAGLYGNALALDGV
                     DDRLVLPTHIGESADFTFAAWVYWNGGASWQRIFDFGNDTNQYLFLTPSSGSNTLRFA
                     IKNGGSEQIVQTSPLPVGQWTHVAVTLSGNTAALYVNGTQAAVNTSATLNPSDFNPQK
                     NYLGKSQWSADPLFSGLLDEVVIADFALTGSQIAQLVSTNQPAALIGAPLTIVGAAAG
                     SEELSDTVGNRKENAFDRNRKTRWCNDSTVANAWITFDLGAAQTVEKVRLRLYNGASR
                     TYPLKIDIDGQEVFRGNTTMTSGYWETVFSPVSGRYVTVAMTANNSAGTGWFSIWEAQ
                     VWEPLNRAPAFLSKTFSQAAAVEGSPYAGTLADKAADPDGGALVFAKVSGPAWLFVSP
                     DGTLSGIPAD"
BASE COUNT          537 a          766 c          797 g          609 t
ORIGIN      
        1 ggtcggcagg aatgcccgac agcgtgccgt cgggggaaac aaacagccac gccggaccgc
       61 tgactttggc aaagacaagc gcaccgccgt ccggatcggc ggctttatcg gccagtgtgc
      121 cggcgtaagg agagccctcg acagcggctg cttggctgaa tgttttggac aaaaaggccg
      181 gtgcccgatt caagggctcc cacacctgcg cttcccagat actgaaccag ccggtgccgg
      241 cgctgttgtt ggcggtcatc gcaacggtta cataccggcc gctgacaggt gaaaagactg
      301 tttcccaata gccgctggtc attgtcgtat tgccgcggaa gacctcctgt ccgtcgatgt
      361 caattttgag cggataggtt cgactggcgc cgttgtagag ccgcaggcgc accttttcaa
      421 ccgtctgggc cgcacccaaa tcaaaggtaa tccaggcatt ggccaccgtg ctgtcattgc
      481 accagcgggt tttccgattg cggtcaaagg cattttcctt gcggtttccg acagtgtcgc
      541 tgagttcttc cgaaccggca gctgctccga caatggtcag gggcgctcca atcagcgccg
      601 cgggctgatt ggtgctgaca agctgggcaa tctggctgcc ggtcaaggcg aaatcggcga
      661 taaccacttc gtccagcagc ccgctgaaca gcggatcggc cgaccattgg ctcttgccga
      721 gataattttt ctgcggatta aaatcgctgg gattgagggt tgcggaggta ttgaccgccg
      781 cctgcgtgcc gttgacatac agtgcagcgg tattgccgct gagtgttacg gccacatgcg
      841 tccactgtcc caccggcagc gggctggtct ggacaatctg ctcgctgccg ccgtttttga
      901 tggcaaagcg cagggtattg ctgccggagc tgggcgtcag aaacagatat tgattggtat
      961 cgttgccgaa gtcgaagatc cgctgccagc tggcgccgcc gttccaatag acccatgcgg
     1021 caaaagtaaa gtcggcgctt tcgccgatat gggtcggcag cacaagccga tcatcgacgc
     1081 cgtccaaagc cagagcattg ccgtacaggc cggcactgcg cgcggggatg ccgtaacaga
     1141 gcggatgatt gccgttgccg gagctgtcgc gaaaatgatt gtcaaaggtg tatacagcgc
     1201 aggcgctttt gccggagccg gggacttcct gctgatagcc gatgggcgaa atttggtcgc
     1261 gatagacctc tccggcctgc gtcggccagc cgtcatccca ctgcacacgc cggcagtctt
     1321 cgacccagtt gtagatagca tagcgctcca caaacggcgt gatgtccagc atgttaatca
     1381 tagcggcaac agcggcggcc tgctgggcat aggtcgggtc ggcgcagccg gtccagttag
     1441 cgccgttgtt ccattcggtc acccagatag gccggccggt ctggtcgtac acagctttga
     1501 ggaaattgta catctgattg gccgcccctg tgggattggc cggatcatag caccagtagt
     1561 aatgcaccgc cacaaagtca acccgcaggt ctgccgcatc cgcccgctgc ataaacggat
     1621 acagccagct gcttcgtccg ccgtcggtag tcgccggcga gcccacccgc aggcctgttg
     1681 ccagcaggtc aggccacgac cagatcgcat cgccgacggc aatattggcc tgttccgtgt
     1741 gatcgggctc gttgtagccc aagaggtggc tggcgccgcg ggtctgccaa ttttgattca
     1801 agcccggcca ccagcggttc tggcggatgg gtacatattc tttgtccagt gtggagtttt
     1861 gatcgatgtt ccagttgtac caccacttga tatccagatt ctggccgatg tttccggcaa
     1921 tgcctttttt gctgacccac cgccacggaa agacccgcac aaaattgatt ttattgtcca
     1981 gcttgcccgg cagaacgccg atttccaaat cgcagtcttg ggcgacatag ttcttgctga
     2041 agcctgtgcc gttttcgttc tgggcgaaag tggccgtata gccccgcttc aaaataaagg
     2101 agctgatatt gttggccatt gttccgagca tgctggtatt gtaggtcgtg tattgggtta
     2161 aataggccca gctgccggca aagttttctt ccgagaagac ctgcagcggg gcaaatgtcg
     2221 gcggctgggg gataataacc gagccgattc cgtattcgac tacccgcaca ttgaatgctg
     2281 ccacggcggc ggcgccgctg accttgactt ggcctaaata ggatgaaacc gccgttgacg
     2341 gcttgaccga ttccagaaac aggaaggcat ccggcgagtt gaggttaatg gtgcagcccg
     2401 acagcggcgt ctgactggtc aaatgcagtt ccgatacgcc gctgatattc acggttgtgc
     2461 tggttaagct gctgtaggtc agcgactgat tgaccatatc aatatctgcc ttcgccgtca
     2521 ggggcaataa gactgttaag gccagcatga attgcatgca tcggatacag gccgctttgt
     2581 tcagttttat tctatgcata atgtacccta tttgctctct ccgccgtctg cacatccgca
     2641 gccgttttgc cggccggctg ctgctttcgt tattccaaca gtgcggctat gtcctctgcg
     2701 ctgagcggc
//
LOCUS       DAOTBO010000220         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_29721_length_2667_cov_7.235835, whole genome shotgun sequence.
ACCESSION   DAOTBO010000220 DAOTBO010000000
VERSION     DAOTBO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29721_length_2667_cov_7.235835"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..221)
                     /locus_tag="PLE88_13295"
     CDS             complement(63..221)
                     /locus_tag="PLE88_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77482.1"
                     /translation="MAVLRVFGLKMGFLGKFAHRGAQAARILKPFKNGRAHFKAGFWT
                     CGGVGAHF"
     gene            469..>2667
                     /locus_tag="PLE88_13300"
     CDS             469..>2667
                     /locus_tag="PLE88_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HOM77483.1"
                     /translation="MNQTARASLFVSIFLSFFAAQHTWADRITGPTFQRWGQEILTQI
                     QNRYGRGDGLYRNSLTTTWPDYVWGQGVILRALAAAAKVDSAYLQRAEQMAEQLRLHY
                     WCTQNQTSGFNSSYGGCGDRYYDDNAWLALALLDLYELTGNSKYLGWAQETVVFCMSG
                     ENGPADSPNGGIRWHESDTGGASVCSTAPTILANLILYQKTAIEHYKTDGQRLYSWIM
                     NSDLRYDTGLFHETNQGALGYQTAVMTQAAVRLYQITGSETYLAEAERMAAAMEHVFI
                     NRTTQALTQHGKWGGHDMTNAYADLYESDGNSRWLDTAAGYLEFLYVNCIDPSNGLYP
                     AVWNDTSRISSADMIDNASVALAYWRMASTPGGTTPHTWFAERLIGRWMLDETSGTFA
                     ADSSGFGCHGTLCGDSFAFEQNAAAGPWNGALLFDGVDDYIDLPDGFANFRAGMTVSV
                     WAYPTAVKNWARFLDFGNGQYNNNIVFGRYGNSNDLFFEGYDGTASGGQVRATGAIVL
                     HQWQMFTATLDASGNVLLYKDGVQIASGTTAVPSNIQRVNNYIGRSNWSADAYFEGRL
                     DDVRICNYALTPEEVQLLYRTGGQAENPAPPTQTMGTTDVISLEWTAGSLAAAHLLYL
                     GTEQIAVLNATPASPEYKGRQTSTLFRPSLVQDTAYYWRVDEETAEQDIARGRLWTFT
                     TAPYPTQGLFVHYLMDSAFISGSTLLDSSPSQNHALLVGPVSTAGLMADAL"
BASE COUNT          592 a          725 c          699 g          651 t
ORIGIN      
        1 gcgccccaat tgaattatca gcaatttccc ccaaaccccc tcagcagccc tttttgtctg
       61 gctcaaaaat gcgcgccgac gcctccgcag gtccaaaatc cggctttaaa atgcgcacgg
      121 ccgtttttaa atggctttaa aatgcgcgcc gcctgcgcgc cgcggtgcgc aaatttcccc
      181 aaaaacccca ttttcagccc aaaaacccgc aaaacagcca tttttgacaa caccccccgc
      241 caccccctcc caactccccg cttccgaagg ccgcccgccg gttatttctt gacgaagggg
      301 aaggggcttt tgtaaaataa atgtgtgtgg ggaacagttt ggcctatgtg aaatttttag
      361 ctctttattg ggggtatgaa ccccatccag atggggacca ttggtttgtc tgactcaaat
      421 tgtttgacct gaacgaaaaa gatgtatttt gtaatgatcg aggtgtgaat gaatcaaacc
      481 gcacgtgcat ccctctttgt atcgattttt ttgtcatttt tcgctgccca gcacacttgg
      541 gccgatagaa tcactggtcc gacattccag cggtgggggc aggaaattct tacccaaatt
      601 caaaaccggt atggccgggg agatggcctt taccgcaatt ccctgaccac aacctggcct
      661 gattatgtgt ggggtcaagg cgttatactg cgtgccctcg ctgctgctgc aaaggttgat
      721 tctgcctatc tccagagggc cgaacagatg gcggagcaat tgcgtctgca ctactggtgc
      781 acgcagaatc aaaccagcgg atttaattcc tcctacggcg gctgtggtga ccgctactat
      841 gatgataatg cctggcttgc actggctttg ctggacctgt acgaactgac tggaaactcc
      901 aagtatctcg gatgggcaca ggagactgtc gttttctgta tgagcggtga aaatggccct
      961 gccgattccc ctaatggcgg gattcgctgg catgaatcag ataccggcgg agccagtgtg
     1021 tgctccacag ccccgacaat tctggccaac ctgattctgt accagaaaac cgcaatcgag
     1081 cactacaaga cggatgggca gaggttgtat tcctggatta tgaactccga ccttcggtat
     1141 gacaccggcc ttttccatga aaccaaccag ggggcccttg gttatcagac ggcggtaatg
     1201 acccaggccg ccgtccgtct ttaccagatc acgggcagcg aaacttatct tgccgaggcg
     1261 gaacgtatgg ccgccgcgat ggaacatgtc tttatcaacc gaacaacaca ggccctcacc
     1321 cagcacggta aatggggcgg ccatgatatg accaatgcct atgcggattt gtacgaatcg
     1381 gacggcaatt cccgctggct cgatacggcc gccggctatc tggagtttct gtatgtcaat
     1441 tgcatagacc cttccaatgg attgtatccg gcggtgtgga atgataccag ccgcatcagt
     1501 tccgccgaca tgattgacaa tgcttcggtt gcactggcct attggagaat ggcttccact
     1561 cccggcggca caacccccca tacgtggttc gccgaaagac tcataggccg gtggatgctg
     1621 gatgaaacca gcgggacgtt cgccgctgac tccagcggct tcggctgtca tggcacgctt
     1681 tgcggagatt cctttgcatt tgagcagaat gccgcagcag gtccatggaa tggggcactg
     1741 ctgttcgatg gtgttgatga ttatatcgac ttgcctgatg gatttgccaa tttcagggcg
     1801 ggcatgactg tctctgtctg ggcttatcct acggcggtca aaaattgggc ccgtttcctc
     1861 gattttggca atggccagta caataacaat attgtttttg gccgatatgg caattccaac
     1921 gacctgttct ttgagggata tgacggaacc gccagcggcg gccaggtgcg tgcaactggt
     1981 gcgattgtac ttcaccagtg gcaaatgttc accgctacct tggatgcttc cggcaatgtt
     2041 cttctctaca aggacggtgt gcagattgca tccggcacta ctgctgtgcc ctccaacatc
     2101 cagcgggtga acaactacat aggtcgaagc aactggtcgg cggatgccta ttttgaaggc
     2161 cgcttggatg atgtgagaat ttgtaattat gcccttaccc ctgaggaagt gcagctgctg
     2221 tacagaacgg gcggtcaggc ggaaaaccct gctccgccga cccaaaccat ggggactact
     2281 gatgtgatta gtcttgaatg gacggccggc tctctggctg ctgctcatct tctttatctt
     2341 ggaacggagc aaatcgcggt tctgaatgcc acacccgctt cgccggaata caaaggccgc
     2401 cagaccagca cgctcttcag accctcgctc gtgcaggaca ctgcctatta ttggcgggtg
     2461 gatgaagaaa cagccgaaca ggatattgcc cgtggtcgtc tttggacatt tactacggcc
     2521 ccttatccaa cgcagggact gtttgttcat tacctgatgg actccgcgtt catcagcgga
     2581 agtactctgc tggattcatc tccctcacag aaccatgcgt tgctggttgg tcctgtgagt
     2641 actgccggac tgatggcgga tgccctg
//
LOCUS       DAOTBO010000221         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_29838_length_2659_cov_8.877880, whole genome shotgun sequence.
ACCESSION   DAOTBO010000221 DAOTBO010000000
VERSION     DAOTBO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29838_length_2659_cov_8.877880"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1454)
                     /locus_tag="PLE88_13305"
     CDS             complement(57..1454)
                     /locus_tag="PLE88_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOM77484.1"
                     /translation="MQTAKLSDFTAVLKPPAPIPSGPPALPPDEDAQRKARAALYAAA
                     ADHRAAGQGRNTKAYLLSAQLREKFGLAEADRLEILRLWNAGNRPPLDDAELAQAVRN
                     PDRYAKRPAGAGLQPGRLQAGLRPEPADKPLEALPLIRLSGVQAESVSWQWPNRFPAK
                     SINLIPGQGAAGKTTFAAYLMACLTTGRDWADCPNTTPPGTCLFIGDEDDLASCIRPK
                     LDAAGADAEKVVALDYDAFFKTAGQSFSLDAHAAMLDKTIADIGDVRAVFFDPLNGYL
                     GRINSYNDGQARSVLIPLQNIAKQHNIAIYGLCHLNKKCDLSALERVLGSVAFVNTSR
                     ATWFITWDKKTDIRYLTCEKSNYTIHPTNLSFRIIDGACSFLEGTTDKTADAVLQAGR
                     QQSDGTLECIEWLRKKLSYNSELSSEISQQARHEGFSRYALDEAKRTLKVVSRRDGYG
                     GKWRLYLPDAADGGR"
     gene            complement(1432..2181)
                     /locus_tag="PLE88_13310"
     CDS             complement(1432..2181)
                     /locus_tag="PLE88_13310"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HOM77485.1"
                     /translation="MPQANQIQRIRQPSSRFWSSRPTQGAGLGASLSVAGSGNPRRPS
                     EAQSAGVQSPTVRDIQAVAGLLFEPADIVELRAIGARCRRMRIVKRWTPAADLPAMAD
                     ELAALNRQGFGIYFGVNPRRQSGCSGDSNVRLARCLFADMDDVEPGDGCGRFEFVYTD
                     LMAAGLPEPTLAVHSGHGIHLYWRLDEPLDDLVLWQRIQSQLNERLGADKAIKNPERL
                     MRLPGFWNTKRQPYQDCFICWGVSYANGKIV"
BASE COUNT          511 a          809 c          743 g          596 t
ORIGIN      
        1 caaattttcc atagatacag gtatttacat atattcacat ataattccct catagactac
       61 ctgcccccat cggcggcatc aggcagatag agccgccatt taccgccgta gccatcccgt
      121 ctggatacca ccttcagcgt ccttttggct tcatccagtg cgtaccggga aaatccctcg
      181 tgccttgcct gctgggagat ttcgctggac aattccgaat tgtaagacag ctttttccgc
      241 agccattcta tacattcgag cgtgccgtca gactgctgcc gtccggcctg taaaaccgca
      301 tcggccgtct tgtcggtggt tccctcgagg aatgaacagg ccccgtcaat aatccgaaac
      361 gacaggtttg tcggatgaat cgtataattc gatttttcac aggtcaggta gcgaatatcg
      421 gttttcttat cccatgtgat aaaccatgtc gctcggcttg tgttgacaaa cgccacagag
      481 cccagcaccc gttccaaggc cgacaggtcg catttcttgt tcaggtggca caggccgtaa
      541 atggctatat tgtgctgttt ggcgatgttc tgcagcggaa tcagcaccga gcgggcctgt
      601 ccgtcgttgt agctgttgat gcgtcccaga tagccgttga gcgggtcgaa aaagaccgcc
      661 cgcacatcgc cgatgtcggc aatggttttg tccagcatgg cggcgtgggc gtccagactg
      721 aagctttggc cggccgtttt gaagaaggca tcgtaatcca gcgcgaccac tttctccgca
      781 tccgcccccg ccgcatccag cttgggacgg atgcacgagg ccagatcgtc ttcatcgccg
      841 ataaagaggc acgtgcccgg cggcgtggtg ttggggcagt cggcccagtc gcgtccggtt
      901 gtcagacagg ccatcagata cgccgcaaag gtggtcttgc cggctgcccc ctgtccgggg
      961 atgaggttga tgctcttggc ggggaaacgg ttgggccact gccagctgac cgactcggcc
     1021 tgcacgccgg acagccgaat cagcggcagg gcttccaacg gcttgtccgc cggttcaggc
     1081 cgcaagcctg cctgcaagcg ccccggctgc aagcccgctc cggccggccg cttggcatag
     1141 cggtcggggt tgcggacggc ttgggccagc tctgcatcgt ccagcggcgg cctgttgccg
     1201 gcattccaca gccgcaggat ctccagccgg tcggcttcgg ccagcccgaa cttctcgcgc
     1261 aactgggcac tgagcagata ggccttggtg ttgcgtccct gcccggctgc ccgatggtcg
     1321 gcagccgccg catacagggc cgcccgtgcc ttccgctggg catcctcatc cggcggcagt
     1381 gccggcggcc cggacgggat cggtgcagga ggtttcagga ctgccgtaaa gtcagacaat
     1441 tttgccgttt gcataggata ctccccagca gataaaacag tcttgatagg gttgccgttt
     1501 ggtgttccag aaaccgggca ggcgcatcag ccgctcggga tttttgatgg ccttgtccgc
     1561 ccccagccgc tcgttgagct gcgactgaat ccgctgccac aaaaccaagt cgtccagcgg
     1621 ctcatccaga cgccaataga gatgaatccc gtggccgctg tggacggcca gcgtcggctc
     1681 gggcagtccg gccgccatca ggtcggtata gacaaactca aagcggccgc agccgtcgcc
     1741 cggttcgaca tcatccatat ccgcaaacag acaccgtgcc agccgcacat tgctgtcgcc
     1801 gctgcagccg gactgccggc ggggattgac gccaaaatag atgccaaaac cctgccggtt
     1861 gagggcggcc agttcgtcgg ccattgccgg caggtccgca gccggcgtcc agcgcttcac
     1921 aatgcgcatc cgccggcacc gggcgccaat ggcacgaagt tccacaatat cggccggctc
     1981 aaacagcagt ccggcgacgg cttgaatatc cctcactgtc gggctttgca cccccgccga
     2041 ctgcgcttcc gacggccttc tgggattgcc gctgccggcg acggataggc tagcgcccaa
     2101 acctgccccc tgcgtcggcc ggctgctcca gaaccggctc gatggctgcc ggatgcgttg
     2161 gatttgattt gcctgcggca ttgttttagc ccttttctcc cttgttgctg ctgcttatac
     2221 catctttttt ccttctcttg tttccggctg ccccgctgca atcgtatcca gcagcacggc
     2281 tgccactgcc gccgcataag cccgataagc catccgggcc gctgggggac taatatactg
     2341 tgtccgcccc tgcaaagcaa caagaaaatg ctaatacaaa aaaatatttg ttaattatct
     2401 aacagaacat tcaggatata ttattcattg attggctgat gccaccgtta tttacatacg
     2461 gcttgcggcg gggattgcat ctgagacctg taaatacctg cgcctatgga tttttagctc
     2521 attatccata ggcgcagact gtgagtatgg atttttagcc aaattttcca tagatacagg
     2581 tatttacata tattcacata taattcccta agggggggtc ttctgctgcc atcgtttagg
     2641 cggtttgtgc tgcggccgg
//
LOCUS       DAOTBO010000222         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_29964_length_2651_cov_4.643683, whole genome shotgun sequence.
ACCESSION   DAOTBO010000222 DAOTBO010000000
VERSION     DAOTBO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29964_length_2651_cov_4.643683"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLE88_13315"
     CDS             <1..194
                     /locus_tag="PLE88_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77486.1"
                     /translation="VSFDWLGKGLPINGQQFEVYDLQSPFSPWIGTTICIPEPATSFI
                     MFLGMLAVVARRSVLCPAA"
     gene            283..1221
                     /locus_tag="PLE88_13320"
     CDS             283..1221
                     /locus_tag="PLE88_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77487.1"
                     /translation="MNYPENEPAYADNFLHLFDDGGTGLYTLYYEPLNLPPAVSRITA
                     AKLTAVSCQPLPLTVTYQTQTGIDESTLDDGDIAVIGQQGQPLTVHFRQVLLRQGQTI
                     EVLYHILPPNGLWEAGANGLYAVSLGAGQVAGRFGIFAEPALLGGFAVSIPACVNLEV
                     SRSQLRSQRRISSSIFEFTYAAIVKNHCSLPVRNLHVIPLSVPANLELVAHDLRFCYI
                     PANGEAVSSGTFTVRMDYTNPPDPQEPFGWQWVLFDSADVTMDGTVDLADLAAFAAAW
                     LTDNSCYDWLPQTQVDGRVDLNDFGILSEHWKTANP"
     gene            1470..>2651
                     /locus_tag="PLE88_13325"
     CDS             1470..>2651
                     /locus_tag="PLE88_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77488.1"
                     /translation="MAVIILGFAATASAAVSADESFILAQVAGTEDILIADFEGDDYG
                     DWQVRGTAFGFKPASGTLPGQMKVAGYTGRGLVNSYCGGDAGTGTLTSPDFAIRRPFI
                     NFLIGGGGYAGQTCMNLLVNGRIVRTAAGPNARPGGSERLDWHTWDVSDLIGKTARLQ
                     IVDDSTGGWGHITVDHICQSQTRRQLGEKTCSFAIQKRYLNFPVKNGNPKRTIQLSCQ
                     GKVIRDFEIELASQEPDFWVFADVSEFNGQTLSLTIDRYDPACTTGFDAVFQDDAFPG
                     QEMLYHEPLRPQFHFSSRRGWLNDTNGLVYYDGEYHLFYQHNPYGWQWGNMSWGHAVS
                     PDMIHWKELPAALHQDALGMIYSGSAVVDEYNTAGFQTGREKPLVAVYTSAGGENPLS
                     KG"
BASE COUNT          557 a          703 c          767 g          624 t
ORIGIN      
        1 gagtatcttt tgactggctt ggaaaaggtc tgcccatcaa tgggcagcaa tttgaggtct
       61 atgatctgca gtcgcctttt tccccttgga ttggcaccac aatttgcatt cccgaaccag
      121 cgacgtcgtt tatcatgttt ctcggcatgc tggccgtcgt cgcccggcgg tctgttctat
      181 gtccggctgc ctaatcccgg ctggccgaat tatgctctca gatgtgttga gcgctcggac
      241 ggcaagacgc tgctgacgga aaacttctgg acaacataca agatgaatta tcccgaaaat
      301 gaacccgcct atgctgacaa tttccttcac ctgtttgatg acggcggtac gggactttat
      361 acactgtact atgagccgct gaatctgccg cctgcggtca gccgcatcac agctgccaaa
      421 ctgacggcgg tatcctgtca gcccctgccc ttgaccgtta cctatcagac tcaaaccggc
      481 atcgatgaat ccacgctgga tgacggcgac attgctgtca tcggtcagca gggacagccg
      541 ctgacagtgc acttccggca ggtcctgttg cggcagggcc aaacgataga ggttctgtat
      601 catatcctgc cgccgaacgg cctgtgggaa gccggagcca acggcctgta tgccgtttcg
      661 ctgggggccg gtcaggttgc cggtcgtttc ggtatttttg ccgagccggc tttattgggc
      721 ggctttgccg tttcgattcc ggcctgcgtc aatctggaag taagccgcag ccagctgcgc
      781 agccagcggc gcatctcgtc gagcattttt gagtttacct atgcggcaat tgtgaaaaac
      841 cattgctcct tgcctgtgcg caacctgcat gtaattccgc tgtccgtccc ggccaatttg
      901 gaactggtgg ctcacgattt gcggttttgc tatattcccg ccaacggcga ggcggtatcc
      961 tccgggacat ttaccgttcg gatggactac accaatccgc ctgatcccca agaaccattc
     1021 ggttggcaat gggtgctgtt cgacagtgcc gatgtgacaa tggatggaac tgtggatttg
     1081 gcggatttgg ccgcgtttgc agccgcatgg ctgaccgata acagctgtta tgactggctg
     1141 ccgcaaaccc aagttgacgg ccgcgttgac ctgaacgatt tcgggatatt gtccgagcac
     1201 tggaaaactg ccaatcctta aagcgccgca gcggattgcc ggccaagcgg cattgttcag
     1261 ccggtcattc ggatgctgcg cttacagcgg ccgcccgatg caaggggggc atcttggggt
     1321 ttgggttgcc gggctttcgg gcatggcgca caacagcggg tacaaaactg cccgcggcaa
     1381 gtcggcgctt tacaaaaccc cagcaagtgt gtacatatgg gcttaaaacg gccttattct
     1441 caacggtggg atagatgcga caatcgccta tggcagtaat aattctggga tttgccgcca
     1501 ccgcgtctgc agcggtgtct gctgatgagt cttttatatt ggcacaagtt gccgggacag
     1561 aggatatcct gattgccgat tttgaaggcg atgactatgg ggattggcag gtcaggggga
     1621 cagcgttcgg tttcaaaccg gcatccggca cactgcccgg ccaaatgaaa gtagccggct
     1681 atacgggcag aggtctggtc aacagctact gtggcggcga cgccggaacc ggcacactga
     1741 cttcgccgga ttttgcaatc aggcggccat ttatcaattt cctgattggc ggcgggggat
     1801 atgccgggca aacgtgtatg aatctgctgg ttaacggccg gattgtccgc acagccgcag
     1861 gcccgaatgc gcggcccggc ggctcggagc gtctggattg gcatacatgg gatgtcagcg
     1921 atcttattgg caaaacagcc cgccttcaga ttgtagacga cagcaccggc ggctgggggc
     1981 atatcaccgt cgatcatatc tgccagagtc aaacgcggcg gcagcttggc gaaaagacat
     2041 gctcctttgc cattcaaaaa cggtatttga attttcctgt caaaaacgga aatcccaaac
     2101 gaaccattca gctgagctgt cagggaaagg tgattcggga tttcgagatt gaactggcct
     2161 cgcaggagcc ggatttctgg gtgtttgcag atgtcagcga attcaacggc caaacgctta
     2221 gtctgacaat cgaccgctat gatccggcct gcacaacagg ttttgacgct gtatttcagg
     2281 acgatgcctt tcccggacaa gagatgctgt accatgagcc gctgcggccg cagtttcact
     2341 tttccagccg gcgcggctgg ctgaacgata ccaacggatt ggtctattat gacggcgaat
     2401 atcacttgtt ttaccagcac aatccttacg gctggcagtg gggcaatatg agctgggggc
     2461 acgccgtcag tccagatatg attcattgga aggaactgcc ggcggcgctg catcaggatg
     2521 cgttgggaat gatttattcc ggttcggcgg ttgtggatga atacaatacc gccggttttc
     2581 agaccggccg cgagaaaccg ctggtggccg tgtatacttc ggccggcggt gaaaatcctt
     2641 tgtccaaagg a
//
LOCUS       DAOTBO010000223         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_30937_length_2584_cov_4.294187, whole genome shotgun sequence.
ACCESSION   DAOTBO010000223 DAOTBO010000000
VERSION     DAOTBO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30937_length_2584_cov_4.294187"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..939)
                     /locus_tag="PLE88_13330"
     CDS             complement(223..939)
                     /locus_tag="PLE88_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOM77489.1"
                     /translation="MIKDHILERYLKALLSGDRMQCRTVIEETLQSGVPAHKVYIDII
                     WPIMVEIDKLYRQDRIDSAQEAFASRINRTIVNQLQNKLPRRPGKNKRVVVVTARTEQ
                     AELGGQMINDLFESDGWETRFLGGSVSHDDILSFIHGYAPDILLLYGFNGQDAPTVRM
                     LIDSIRNVNAFPQMRIMLSGGVFSRAEGLWEEVGADLYADTAAEAVMMAQMPAEQLPK
                     PLRTIKQRYYEEEHQESLAR"
     gene            1456..>2584
                     /locus_tag="PLE88_13335"
     CDS             1456..>2584
                     /locus_tag="PLE88_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77490.1"
                     /translation="MGDLMIKAIIGWVCIVFLCVLPVYGVNTNEIEALRQRVQQSGIE
                     LSAADTAVISKFWSAALDQMLLSANSQDMVEVRRQLEAQRGNEYLSFYATAYIAEALK
                     DIQAAYQNTQRIENLQQRINAERNLMILTANLASPKLAPIAVSRLADTDEVIRYWAVK
                     AITQPAVIEQLSKEEVTRDPQAAEAILNALKERILSEPLTEIQKMMIDFAAAFNHPAA
                     RQILLSLSEQRLNAYRQWKVQDELVETKLLTALGTIAVLQQDAEIKKTFGKAFAELYS
                     AVFQRYMKGAARLSAEQTEQLIAVISEIDQTVVNKTMGIQTSILRTLQRRGPLDSDYE
                     MLFGNRQLAGELGLRFKFDYGKDASGKPITFPAELDPMPEAA"
BASE COUNT          741 a          521 c          582 g          740 t
ORIGIN      
        1 taatgcagac aggctgcaga atttgtgtca aaagcatttt ttattgatta cctgttatat
       61 aaacaaacat actattttta cggacgatta aagaacaact atatagcgtt ttgccggcga
      121 taatgcaaat attttatctg caaaatttcc aatagtctac aaatagcatc ggttattaca
      181 aaaaaagccg gataaaaccc ggcttttaag agatatatga aattaacgtg ccaaactttc
      241 ttggtgttct tcttcgtaat aacgctgctt gatggttcgc agcggcttgg gaagctgctc
      301 ggccggcatc tgtgccatca ttacagcttc agcggcggtg tctgcatata aatcggcacc
      361 gacttcctcc cataaacctt cggctcgtga aaaaactcct cctgacagca taatccgcat
      421 ctgtgggaaa gcgttgacgt tgcggatcga atcgatgagc attcgtacag taggggcatc
      481 ttgtccatta aacccataca gcaacaggat atcgggagca taaccatgga tgaatgacaa
      541 aatatcatcg tggctgaccg aaccgccgag aaaacgggtt tcccaaccat cgctttcaaa
      601 aaggtcgtta atcatttgtc ctccaagctc tgcctgttca gtccgggcag ttactacgac
      661 aacccgttta tttttgcctg gacggcgggg aagcttattt tggagctgat taacaattgt
      721 ccgatttatg cgtgaggcga aggcctcttg agccgaatcg attcgatcct gacgatataa
      781 tttatcgatc tctaccataa taggccagat tatatcgatg taaaccttat gggcaggtac
      841 tccggattga agtgtctcct caataacggt tcggcattgc attcggtcac cggaaagtaa
      901 tgctttaaga taacgctcaa gaatgtggtc tttaatcatt tttgcctcac tttccaaatc
      961 ttttaattat attattttga atgttaatat ggttaaattg ggtaaattcg ataaaatttc
     1021 tatcgggggt tcttcaaaaa aaactttagt gaaaatagga aaattgggta atatgatgaa
     1081 aatgataatt tgattttaac aataatataa gtattttaat tctaattact taaagaatgc
     1141 gtcgtaaaaa ttaagaccta taaatttttt atttttaatc gataaaatag ttttaaaaat
     1201 ctttttttaa ccaatttaac ttacttatga atcattctta gataaaacta attagattgg
     1261 gtagtatttt tatgctttta gtgaaaggct gaactttaag ataatagata ttttttttaa
     1321 tttttcccct gtcaaaagga atttttccat caagcgaatt gtgttgcatc ggctggttat
     1381 ttgcttgaca tagactaaca ataccggtaa tgtacccaaa tctatgttgc gatagcactt
     1441 aaagattttt tttcgatggg tgatctaatg ataaaagcca ttatcgggtg ggtatgcatt
     1501 gtctttttgt gtgttttgcc ggtttacggt gtcaatacaa acgagattga ggcgcttcgt
     1561 cagcgtgttc agcagagcgg catagaatta tctgctgcgg atacagcggt tatttcaaaa
     1621 ttctggtcag cagctctgga ccagatgctg ttatcggcca actcgcagga tatggtggaa
     1681 gtgcgtcgac agctggaagc gcagagagga aatgaatatt tgtcgtttta tgcgacggct
     1741 tatattgctg aagcattgaa agatatacaa gcggcttatc agaataccca gcgaattgaa
     1801 aatttgcagc agcgcatcaa cgccgagcgc aacctcatga ttctgactgc caatctggcc
     1861 agtcccaaat tggcgccgat agccgtcagc cgattagccg atacggatga agtgatacgt
     1921 tactgggctg tcaaggcgat aacgcagccg gctgttatcg agcagttatc caaggaggaa
     1981 gtaacgcgcg acccgcaggc tgcagaagcc attttgaatg ccttgaaaga acgcatttta
     2041 agcgagccgc tgacggaaat acagaaaatg atgattgatt ttgccgccgc atttaatcat
     2101 cccgcagcgc ggcagattct gctgtcgctg tccgaacagc gattaaatgc ataccgccaa
     2161 tggaaggttc aggatgagct tgttgagact aaactgctga cagctctggg aacgattgct
     2221 gtgcttcagc aggatgccga aatcaaaaaa acattcggca aggcgtttgc tgaattgtat
     2281 tcggcggttt ttcagcgtta tatgaaagga gctgcccggc tttctgccga acagaccgag
     2341 cagctgatag ccgtcatctc agagattgac caaaccgtcg taaacaaaac gatgggcatc
     2401 cagaccagca ttctccgtac gcttcagcgg aggggacctc tggattcgga ttatgaaatg
     2461 cttttcggca atcgccagct ggcaggggaa ctgggactgc ggtttaaatt tgattacggc
     2521 aaagatgcgt ccggcaagcc gattacattt ccggcggaac tggacccgat gcccgaagcc
     2581 gcaa
//
LOCUS       DAOTBO010000224         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_31520_length_2546_cov_3.892814, whole genome shotgun sequence.
ACCESSION   DAOTBO010000224 DAOTBO010000000
VERSION     DAOTBO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31520_length_2546_cov_3.892814"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLE88_13340"
     CDS             <1..582
                     /locus_tag="PLE88_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOM77491.1"
                     /translation="DRHGFEPDYRTPENEDARYSPGTVQESCQHVGYQWAWRPNDRVP
                     SVKWFIKRIVINASRNSNILMNIGPSPDGTFDPKQADRIREVGRWLSHHAEAIYGTRG
                     GPVIDNSDQPAFVTTRSGNRIFVHVLQDTSAGSEIRLPGVMISSAVRFGYPDQSLPFR
                     QGNDAGFLRLPETLDPLDEIIVIEGTFQADEVL"
     gene            613..>2546
                     /locus_tag="PLE88_13345"
     CDS             613..>2546
                     /locus_tag="PLE88_13345"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOM77492.1"
                     /translation="MKEHHMKITIAGVFAAAACFMPIEKSYSKPAGEVITLSRSPLVW
                     RIETAVSVYELGVADDGIVVPVYYGPKGQIREIRDLPLQVDPIIGSKIREVPYRGGFV
                     EQTPGLEVVYADHTRDCELVYQSHEIVEIGGYPCLRIDMADKFYQLEVSSFIRVIPDK
                     DILEKWLSVKNQSDKAVLLENAQSGSVWLEPDEYELLHFSGQWSNEFMMHKTRLTPGV
                     KTLQVRDFFSWTSPWFAVMPEGRTDEHSGPVWFGQAAYAGNWRMDFDKSSSGNLQIIG
                     GINFWDTALTLKPQDTFQTPKMIFGFSPNGLNGASQRLHDYIRNDVIRKETRNKIRPV
                     LYNSWFATEFDVQEDQQLKLVERAKDIGVELFVIDDGWFKGRISDKAGLGDWEVDKNK
                     FPNGLKAFIEKVNQMGMEFGIWIEPEMANKDSDLYRAHPDWVLHYPNRVSHEHRNQLM
                     LNLARQDVCDYLFAQFNKLLSENKIAFIKWDRNRALSEAGWPDAPADVQREVRLRYID
                     NLFKLIDALQASHPDVLFETCSGGGGRINLAMLSRMDQAWPSDNTNPSDRVMMQYGYL
                     HAFPANTMASWVTDVDWHRAAPPLEYRFDVAMAGVLGIGSDITKWSRQDVETAKKKIA
                     QYKHIRDVVQNGTLYRLESP"
BASE COUNT          616 a          613 c          735 g          582 t
ORIGIN      
        1 gaccggcacg gtttcgagcc ggactatcgg acgccggaga atgaggatgc gcgttatagt
       61 cccgggacgg tgcaggagtc ttgccagcat gtcggctacc agtgggcgtg gcggccgaat
      121 gaccgtgtgc cttccgtaaa atggtttatt aagcggattg tcataaatgc ctcccgaaac
      181 agcaatattt tgatgaacat aggcccttcg ccggacggca cgtttgaccc gaaacaggcg
      241 gaccggatca gagaagtcgg ccgctggctg tcgcatcacg ccgaagcgat ttatggaact
      301 cgcggcggtc cggttattga taacagcgat cagcctgcct ttgtgacaac ccgcagcggc
      361 aatcggattt ttgtgcatgt cctgcaggat acatcggccg gcagtgaaat acggcttccg
      421 ggtgttatga tttcttctgc cgtccgcttc ggctatccag accaatccct gccttttcgt
      481 caaggaaacg atgccggatt tttacgtttg cccgaaacgc ttgatccgct ggatgaaata
      541 attgtgattg aagggacatt tcaagccgat gaagtgttat agtatagggc agcaggataa
      601 aggattacgt gaatgaaaga acatcatatg aagataacaa ttgcgggggt ttttgctgcg
      661 gcggcgtgtt ttatgccgat tgaaaagagc tattcgaagc cggccggcga ggtcattacc
      721 ctttcacggt cgcctttggt gtggcggatt gaaaccgccg tctcggtcta tgagcttggg
      781 gttgccgatg acggcattgt ggttcctgtt tattacggcc ccaaaggtca gataagggaa
      841 atccgggatt tgccgctgca agttgatccg attatcggca gcaaaatccg ggaagtgcct
      901 taccggggcg gatttgtcga gcagacgccg ggactggagg ttgtctatgc cgaccacaca
      961 cgcgattgcg aactggtgta tcaatcgcac gagattgttg aaatcggcgg gtatccttgc
     1021 ctgcgaatcg atatggccga taaattctat caactggaag tgtcttcgtt cattcgggtt
     1081 attcctgata aggatattct ggaaaaatgg ctgtctgtga aaaatcaatc cgataaagca
     1141 gttctacttg aaaacgccca atccgggtct gtctggctgg agccggatga atatgaactg
     1201 cttcattttt caggccagtg gtccaatgaa tttatgatgc acaaaacccg cctgacgccc
     1261 ggcgtcaaga cccttcaggt gcgcgatttc ttctcatgga caagcccttg gtttgcggta
     1321 atgccggagg gccggacgga tgaacacagc gggccggtct ggttcggcca agcggcatat
     1381 gccggcaact ggcggatgga ttttgataaa agcagttcgg gcaatctgca gattatcggc
     1441 ggcatcaact tctgggatac tgccctaacc ctgaagccgc aggacacttt tcaaacgccc
     1501 aaaatgattt tcggattcag ccccaatgga ttaaacggcg cctcgcagcg gctgcatgac
     1561 tatatccgaa atgatgtgat tcgcaaagag acgcgaaata aaatccgccc cgtgctgtac
     1621 aacagctggt ttgcgacgga gtttgacgtt caggaggacc agcagctcaa actggtcgag
     1681 cgcgccaaag acatcggggt ggaattgttt gttatcgatg acggctggtt caaaggacgc
     1741 atcagcgaca aggccggact gggcgactgg gaagtagata aaaacaaatt cccgaatgga
     1801 ctgaaagcat ttatcgaaaa agtgaatcaa atgggaatgg agtttggaat ctggattgag
     1861 ccggagatgg ccaacaaaga cagcgacctg taccgggcgc atccggattg ggtgctgcat
     1921 tatccaaaca gggtatcgca cgagcatcgc aatcagctga tgctgaatct ggcccggcag
     1981 gatgtctgtg attacctctt tgcacaattc aacaaactgc tttccgaaaa caaaatcgcc
     2041 tttatcaagt gggaccggaa ccgggcactt tcggaagccg gctggccgga tgcgccggct
     2101 gatgtgcagc gggaggtccg tctccgatat atcgacaacc tttttaaact gattgacgcc
     2161 ctgcaggcca gccatcccga cgtgctgttt gaaacctgct ccggcggcgg cggacgcatc
     2221 aatctggcga tgcttagccg gatggatcag gcatggccca gcgacaatac caatccttcc
     2281 gacagggtga tgatgcagta cggttatttg catgcatttc ccgccaatac gatggccagc
     2341 tgggtgacgg atgtcgattg gcacagggcc gcaccgccgc tggaataccg gtttgatgtg
     2401 gcgatggccg gggttctggg catcggctcg gatattacca aatggagccg gcaggatgtg
     2461 gaaacggcaa agaagaagat tgcacagtat aagcacatac gggacgttgt gcagaacggc
     2521 acgttatacc ggctggaatc cccctt
//
LOCUS       DAOTBO010000225         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_31675_length_2537_cov_3.595488, whole genome shotgun sequence.
ACCESSION   DAOTBO010000225 DAOTBO010000000
VERSION     DAOTBO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31675_length_2537_cov_3.595488"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1431
                     /locus_tag="PLE88_13350"
     CDS             37..1431
                     /locus_tag="PLE88_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77493.1"
                     /translation="MSRKTWLVWALVLLLLGAVGLSTYCAQKKTWQERIAQAAKADSQ
                     DPAGPEYLKIYEEWSNLPAEEKADNPWGYNLYGGAEIRNRLVEGQANRLLRDLPKLAK
                     GLTDYPDELIENLYGSNWQEEVEKYRAALNHGEILLAASTFLLACGGLILGGAAAKTL
                     FAAVIGKSKKKKEDAYPPISAPEVQSAGAKDNIENAASNKENKAAAVEDPQPDCRKTE
                     ALESCSDASPETSGRQNTKAGSEEGYFQAFRKSSKKVSADSGTKETALPLTAFSQKAL
                     LDKPAKDSYFGWAVEMETSSPLESLMTTEPLTRELTELTEEVSAIRQFAAQQQDQMRK
                     LQDGYDWVIIRRFCLRIIRSIDNIEDRIAALDSSQTDTAACLQDIRDELVFALESSGI
                     EQFEPDLNIPYKGLEKYAEAVRQRIPTDNPELVGCIARVVRPGYQYLINDDNVKIVRC
                     AQVNLYEAKQSLQE"
     gene            1436..>2537
                     /locus_tag="PLE88_13355"
     CDS             1436..>2537
                     /locus_tag="PLE88_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOM77494.1"
                     /translation="MSTIAGIDLGTIFSALARLNGIGRPEIVPNSEGERITPSAVYFD
                     EEEDGAIRIGLEAINCRQLNPERAVRWIKRHMGDPAWKKTIDGRDWTPQEISSLILKK
                     LKQDCSVEGEIKDVVISVPAHFDETRRKATMDAGTLAGLNVIGIINEPVAAALYYATT
                     QKVCGRVMVYDLGGGTFDVTILDVDGSSIDIVCSQGDHALGGVDFDKKIIEMFEDAYR
                     KEYRTELIQNEQDRAKYEDEAEDIKKTLSRRNIVRKLLYGPAGSMRIEITRQQFEEAV
                     EPLMGRIEMLIEVALEEAKCTVKDIHKVLLAGGSTRMPIVQQRLEKKFGFPPEIAVNV
                     DECVALGAAIHAGLTLLRDNPQKVPAGIAAGLK"
BASE COUNT          698 a          578 c          706 g          555 t
ORIGIN      
        1 gtacagcgat ggttttcctg ttaggcattc agcataatga gtagaaagac atggttagtc
       61 tgggctttgg tattgctctt gctcggcgca gtggggctga gtacttactg cgcacagaaa
      121 aaaacctggc aagagcgtat tgcccaagcg gccaaagctg actcacaaga ccctgccggc
      181 cccgagtatt tgaaaatcta tgaagaatgg tcaaatcttc cggcagaaga aaaagccgat
      241 aatccttggg gctacaacct ttatggggga gctgaaattc gcaatcgcct tgtggaagga
      301 caagccaacc gcctgcttcg agatcttccg aaattagcca aaggattaac agattatcct
      361 gatgaactta ttgaaaacct gtatggatca aattggcagg aagaagtaga gaaatacagg
      421 gccgctctga atcatggtga aatacttttg gcggcttcaa cctttctgct ggcctgcggc
      481 ggacttattt taggaggagc agctgccaag acgctctttg cggctgttat cggcaaatcc
      541 aaaaagaaaa aagaagatgc atacccgccc atatcagcac cagaagtcca atctgctggc
      601 gccaaagaca atatagaaaa cgctgcatct aataaagaaa acaaagctgc tgctgttgaa
      661 gatccccaac cggactgcag aaaaaccgaa gctttggaaa gttgctctga cgccagccct
      721 gagacaagcg gcaggcaaaa cacaaaagcc ggcagcgagg aaggctactt tcaagcgttt
      781 cggaaatcat ccaaaaaagt gtcggcagac agcggcacaa aggaaacagc attgccgctg
      841 acggcgtttt cacaaaaagc tctgcttgat aagcctgcca aggattcgta cttcggctgg
      901 gcggttgaaa tggagacgtc ttcgccgctg gagtcgctga tgacgaccga gccgctgacg
      961 cgggagctga cggagttgac agaggaggtg tcggccattc ggcagtttgc ggcccagcag
     1021 caggatcaga tgcgcaagct gcaggacggc tacgactggg taatcattcg gcgattctgt
     1081 ctgcggatta ttcggagcat tgataatatt gaggaccgca ttgcggcgct ggattccagc
     1141 cagacagata cggcagcctg ccttcaggat atccgggatg agctggtatt tgcacttgaa
     1201 tccagcggca ttgagcaatt cgagccggat ttgaatattc catataaagg acttgaaaaa
     1261 tatgcggaag ccgttcgcca gcggattccg acagacaatc ccgaattggt cggctgtatt
     1321 gcccgggtgg ttcgtcccgg ctatcaatat ttgattaatg atgacaatgt taaaattgtt
     1381 cgctgcgcac aggtaaacct gtacgaagca aaacaaagtt tacaggaata ggaatatgag
     1441 cacaatagca ggaattgatt tgggtactat cttctctgca ctggcccgtc tgaacggcat
     1501 cgggcgtccg gaaatcgtgc caaacagcga aggcgagcgg attacaccgt cggcggtgta
     1561 ctttgatgaa gaagaggacg gcgctatccg catcggcctg gaagccatca actgccgtca
     1621 gctgaatccg gaacgggctg tccgatggat taagcggcat atgggcgatc cagcatggaa
     1681 gaaaaccatc gatggacgcg actggacgcc gcaggaaatt tccagcttaa tcctcaaaaa
     1741 gctgaagcag gactgctccg tcgaaggtga aatcaaagat gtggttattt ctgttccagc
     1801 acattttgat gaaacccgcc gcaaagctac gatggatgca ggcaccttgg caggtctgaa
     1861 tgttatcggg attatcaatg aacctgtggc tgcagccctg tattatgcca caacccagaa
     1921 agtctgcggc agggtaatgg tgtatgacct cggcggcggc acttttgatg taaccattct
     1981 ggatgtggac ggctcatcga ttgatatcgt ctgcagtcag ggcgatcatg cgctgggcgg
     2041 tgttgatttt gataagaaaa tcattgagat gtttgaagat gcgtatcgaa aagagtaccg
     2101 cacagaactg attcaaaatg agcaggaccg tgccaagtac gaagatgagg ccgaagatat
     2161 caaaaaaacg ctgtcgcgcc ggaatattgt acgcaaactt ttgtatggtc cggccggatc
     2221 gatgcggatt gagattaccc gacagcagtt tgaagaagcc gtcgagccgc tgatgggacg
     2281 aattgaaatg ctgattgaag tggcgctgga agaagcaaaa tgtactgtaa aggatattca
     2341 caaagttctt ttggccggcg gcagcacccg gatgccgatt gtccagcagc ggctggaaaa
     2401 gaaatttggt tttccgcctg aaatcgctgt caatgtcgat gaatgcgtgg cattgggggc
     2461 cgcgattcat gccggcctga ccctgctgcg ggacaatccc cagaaggtcc cagccgggat
     2521 cgctgccgga ttaaagg
//
LOCUS       DAOTBO010000226         2499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_32255_length_2499_cov_5.033142, whole genome shotgun sequence.
ACCESSION   DAOTBO010000226 DAOTBO010000000
VERSION     DAOTBO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32255_length_2499_cov_5.033142"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>2499
                     /locus_tag="PLE88_13360"
     CDS             145..>2499
                     /locus_tag="PLE88_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM77495.1"
                     /translation="MLKQYLILQRILKTASVAMLVCALGPVFADYPIMSQHYAADPTA
                     IEWNGRLYVYCSNDEENTENSGYIMNSIVCFSTDDLKNWTDHGVVFDADSVPWIGTAW
                     APCIVRNNNKFYLYFGDPYWGIGVAASDVPTGPFTDIKGNLVVKREGLTGGTPGADST
                     WLFDPCVFVDDDGQPYLYFGGGGANQARVILLNSNMMDPIGSALTIEFPDFFEASLMH
                     KHNGLYYYSYADNYDDSTAGSQIAYMTGTSPLGGFVYRGTALGQLPYNYNNNNHHTFF
                     TYQGQWYCVYHNRYQAGLDGVSTTEHRNICLDRMYYNPDGTIQPIVPTQDGLVQLKYL
                     NPFSRVEGETLAQQSGIKTEVCSEGGLQVTAISHGDWIRIRGADFGSGAGSFLARVAS
                     AVSGAAIELRLDSLSGTLAGTLPVPNTGSTQTWTTAYCNVSGLSGVHDLYLKFTGPAD
                     GDLFKLNWWQFQTEPVTMANVSADSSVLYQTIEGLGGAICFYNGWFTAHPYKQEIFDY
                     AFSGLNLSMLRIGNWWRGTNGQDTAIYEIVAAANQRLGRPVPILISSWSPPAYLKSNG
                     QTTNGGTLIQKDGAYDYAGFADYWYASIQDYIAHGVTPTWIGIQNEPDWTADYDSCRF
                     NPTEDPVSGQYYASFALAQDAVYQKLQSAMASPPKLLSPECVGLYGNAAGLRNYMAAL
                     NPATFYGIAHHLYGGSTDGTPDGYNSAFTAVLNATNTLFAGKPRFMTEFGDIKGLIPV
                     ANLIHNSLVVEQVSGYNHWSLMWPGDIGLVEIEFPWGSSSWTSPK"
BASE COUNT          564 a          652 c          701 g          582 t
ORIGIN      
        1 aaatgcacag caggcagggg aaaaagcgcc tgcggaaggc ttggcaggag taccccgata
       61 aataatgagg acagtattcg gcccggctga tagggatatg tatcggcagg ccggagaacc
      121 aacattcata aatggggcgg gactatgtta aagcagtatc taatcctcca gcggattttg
      181 aaaacagcat ctgttgcaat gcttgtatgc gcactcggtc cggtatttgc ggattacccg
      241 attatgtcgc agcattatgc cgctgacccg acagcaatcg aatggaatgg ccggctgtat
      301 gtgtactgct ccaacgacga agaaaacacc gaaaacagcg gctacattat gaattctatt
      361 gtctgtttct cgacagatga tttgaaaaac tggacggatc acggtgtggt gtttgatgcc
      421 gacagcgtcc cgtggatagg gacggcgtgg gctccctgca ttgtccgaaa taacaataag
      481 ttttacctgt atttcggcga tccttactgg ggcatcggcg tggccgcaag cgatgtgccg
      541 accggtccgt ttacagatat caagggcaat cttgtcgtaa aacgggaagg attgacaggc
      601 ggaactccgg gagccgacag cacttggctg tttgacccct gtgtatttgt tgatgacgat
      661 ggccaaccgt atctctactt cggcggcggc ggcgccaatc aggcacgggt gattctgctc
      721 aattccaata tgatggatcc aatcggctcg gccctgacga ttgagtttcc ggattttttc
      781 gaggcctcgc tgatgcacaa gcacaacggc ctctattact attcctatgc cgacaactac
      841 gacgattcaa cggccggttc gcagattgcg tatatgaccg gcaccagtcc tctgggggga
      901 tttgtttatc ggggaacggc ccttgggcag ctgccgtata actataacaa caacaatcat
      961 catacctttt ttacctatca ggggcagtgg tactgtgtct atcacaaccg gtatcaagcc
     1021 ggactggatg gtgtctcgac gacggagcat cgcaatatct gtctggaccg gatgtattac
     1081 aatcccgatg gaacgataca gccgattgtt cccacgcagg acggccttgt ccagctgaaa
     1141 tatctgaacc ccttcagccg cgtggagggc gagacgcttg cccagcagag cggcatcaag
     1201 accgaggttt gttcggaagg cggtttgcag gtgacggcca tctcccatgg cgattggata
     1261 cgcatacgcg gagcggattt cggcagcggg gcaggcagtt ttctggcccg tgtcgccagc
     1321 gccgtcagcg gcgcagccat tgagctgcgg ctggacagtc tgagcggcac cctggccggg
     1381 acgctgccag tacccaatac gggcagcaca cagacatgga caactgccta ctgcaatgta
     1441 agcgggctgt ccggcgtgca tgatttgtat ctgaagttta ccggtccggc ggatggcgat
     1501 ttgttcaagc tcaactggtg gcagtttcag accgagccgg ttacgatggc aaatgtcagt
     1561 gccgattctt ctgtgctcta tcagacgatt gaaggactgg gcggggcgat ctgcttctac
     1621 aacggctggt ttacggccca tccctacaag caggagattt tcgactatgc attttccgga
     1681 ttgaatctgt cgatgctgcg catcggcaac tggtggcggg gcacaaatgg tcaggatacg
     1741 gcgatttatg aaatcgttgc tgctgccaat cagcggctgg gcagacctgt gccgattctg
     1801 attagttcgt ggtcaccgcc ggcttatctt aagagcaacg gccaaacaac caacggcggc
     1861 acgctgattc aaaaggacgg tgcttacgac tacgcaggat ttgccgacta ttggtatgca
     1921 tccattcagg attatattgc ccatggagtc accccgacct ggatcggcat tcaaaatgaa
     1981 cccgactgga cagccgatta cgattcctgc cggtttaatc cgaccgagga ccctgtcagc
     2041 ggccagtatt atgcaagttt tgccttggcg caggatgcgg tgtatcagaa gctccagtcg
     2101 gccatggcat cgcctcccaa gctgctcagc ccggaatgcg taggccttta cggcaatgcc
     2161 gccggcctgc gcaactatat ggccgcactg aatcctgcta ctttttacgg cattgcccac
     2221 cacctttacg gcggcagcac tgatggcacg ccggatggat ataattctgc atttaccgcg
     2281 gtgctgaatg ccaccaatac gctttttgcc ggcaagcccc gttttatgac cgagttcggc
     2341 gacatcaaag gattgattcc tgttgccaat ctgattcata attccctcgt agtcgagcag
     2401 gtcagcggct ataaccactg gagtctgatg tggccgggcg atatcggatt ggtcgaaatc
     2461 gagttcccgt ggggctccag ctcgtggaca tccccgaaa
//
LOCUS       DAOTBO010000227         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_32662_length_2473_cov_3.805211, whole genome shotgun sequence.
ACCESSION   DAOTBO010000227 DAOTBO010000000
VERSION     DAOTBO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32662_length_2473_cov_3.805211"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..895)
                     /locus_tag="PLE88_13365"
     CDS             complement(2..895)
                     /locus_tag="PLE88_13365"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOM77496.1"
                     /translation="MKSISVKIPFIAVTVLALSGLCSAGVLLYDTFADGSRAETNLPN
                     ESAVWVGTPASVTMNPGSLAYAQGSSSQKLWTYFAPNGSPVELSVGHQLIATITFTPR
                     VKLYDSSSKNFRFGLFYDPTNDQIAADINSDSGGTGDPWKDSTGYAVHFPLSSGPTSI
                     SNSSVGKRIPDLHTSLLGSGSAYPGITSGGAKITATLDTLYTLTFKLDYQAANAMVVT
                     FTLSDASGVLSTNSITDDGTFGGYNMGIYTKFDQLFFRFSSATATADVIDFHSIKVEH
                     IVPEPATLVLLGLGGLMLKRR"
     gene            1207..2403
                     /locus_tag="PLE88_13370"
     CDS             1207..2403
                     /locus_tag="PLE88_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOM77497.1"
                     /translation="MHSRQKIKIAVAHNNISHNTQGIMAGITDFIRTKGDWQLIIWPD
                     SSLESLEFLKQRGCKGAFVSVQTSTKAQQLLSLGIPIIAVSTLQTMLNLPFISADSEQ
                     IAKMAYDYFVDKKFANFAFFGLTEAHWSRQRMEHFSRLAAADGGTVHVYTGPPALITS
                     DITSFARLLLRSELNKSQQELLDWLVQLPKPVAVLASCDLLACHLMIAASDAGLSIPD
                     DIAVLGVDNDEALCNLCDPPLSSIAFNFKKAGCDAAQLLDRLISGQDTMRGQRICILP
                     SEIKSRGSTDIYAISDPDIIKALQYIRANRTQPMQVEDIGRHICISKRSLQLKFQKTL
                     NRSIHDEIVRAHFEVAKALLIETDLPIDEIAVRSGFHYTSNLRRVFVKITGMLPHLYR
                     QLHRPR"
BASE COUNT          606 a          690 c          605 g          572 t
ORIGIN      
        1 gtcagcggcg tttgagcatc aggccgccca gtcccagcag aaccagcgtc gccggctccg
       61 gcacaatgtg ctcaacctta atgctgtgga aatcgattac atctgcggtt gcggttgcac
      121 tggagaagcg gaagaacaat tggtcaaact tggtataaat acccatattg tatccgccga
      181 atgtaccgtc atctgtaatg gagttggttg aaagaacacc gctggcatcg gacagcgtaa
      241 aagttacaac cattgcatta gccgcctgat aatccagttt aaatgtcaat gtataaaggg
      301 tgtcaagagt cgctgtaatc ttcgccccgc cgctggttat tcctggatag gcagaaccgc
      361 tgcccagcaa actggtatgc aggtctggga tgcgcttgcc gacactggaa ttggagatac
      421 tcgtcgggcc tgaactgagc gggaaatgca cggcatagcc ggtagaatct ttccacggat
      481 cgcccgttcc gccggagtca ctgttgatat cagccgctat ctggtcgttg gtaggatcat
      541 aaaacaaacc gaagcggaaa ttctttgacg atgaatcata caacttgacg cgaggtgtaa
      601 atgtaattgt ggcaatcaat tgatgcccaa cagataattc gaccggcgag ccgttgggtg
      661 caaaataagt ccacagtttt tggctgcttg agccttgggc ataggccaaa gagccgggat
      721 tcatcgttac gctggcaggc gtgccgaccc acacagccga ttcattcggt aagttggttt
      781 cggctcggct gccgtcggca aaggtgtcat acagcaagac gccggctgaa cacagtccgc
      841 tcagcgcaag gacggtaacg gcaataaagg ggatcttcac tgaaatgctt ttcatcttcc
      901 tgtcctccaa aaaaagtaat atgggttaaa gcaaactaaa aagtttcttc gacgatttta
      961 aaacttcccg attttaccca actttccaac atttatattc ccacaaaaaa agcttcaaaa
     1021 caagtcaatt ttgcgcttaa ataaaggcga ttttgcgcaa attgtctcgt taacaagcaa
     1081 ccatcgcaaa caaaatacgg cattgtgcat cttcttttgc cctcgcaaat cgtttcctta
     1141 ttttatcctg cttgacttat tttgcgcaat atgatagtat aaaggcattc ggttagaaga
     1201 ggtcccatgc acagccggca gaaaataaaa atcgccgtag cgcacaacaa tatcagccat
     1261 aatacacaag gcattatggc cggtataacc gacttcatcc gtaccaaagg cgactggcag
     1321 ctgattatct ggccagacag ttccttggag tcgctggaat ttctcaagca gcggggctgc
     1381 aaaggcgcct tcgtcagtgt ccagacatct accaaagccc agcagctcct ttcgctgggc
     1441 atccccatta ttgccgtttc cacgcttcaa acaatgctga acctgccgtt tatcagcgcc
     1501 gattccgaac aaatcgcaaa aatggcgtat gactacttcg tcgataaaaa attcgccaat
     1561 tttgccttct tcggcctgac cgaagcccac tggtcccgtc agcgaatgga gcatttttcg
     1621 cgtttggccg ctgccgacgg cggcacggtg catgtttaca ccggcccgcc ggcccttatc
     1681 accagcgaca tcacctcttt tgcccgcctt ctgctgcggt cggaactgaa caaaagccag
     1741 caggagcttc tggactggct ggttcaattg cccaagccgg tagccgtgct ggcaagctgt
     1801 gacctgctgg cctgccatct gatgattgcc gccagcgatg cggggttgtc tattcccgat
     1861 gatatcgcag tgctgggggt cgataacgat gaagccctgt gcaatctgtg cgatccgccc
     1921 ctgtcgagca tcgcgtttaa cttcaagaag gccggctgcg atgccgccca gctgctcgac
     1981 cggctgattt ccggtcagga taccatgcgc ggccagcgga tttgcatcct gccgtctgaa
     2041 atcaaaagcc gcggctcgac cgacatttac gctatcagtg atcccgacat catcaaggcc
     2101 ctgcagtaca tccgtgccaa ccgcacccag ccgatgcagg ttgaagacat cggacggcat
     2161 atctgcatca gcaaacgctc gctgcagctg aagtttcaaa agaccctcaa ccgctccatc
     2221 cacgacgaga ttgtccgggc ccattttgag gtggccaagg ccctgctgat tgaaaccgat
     2281 ctgccgatcg atgagattgc cgtccgctcg ggttttcatt acacctccaa cctccgccgg
     2341 gtgtttgtga aaatcaccgg catgctgccc cacctctacc gccagctcca ccgcccgcgg
     2401 taagtgccgc gcaaaaccgc atccagcgga ttggccggct gtcgtcttcc gacagacctg
     2461 ccgcaacggc aca
//
LOCUS       DAOTBO010000228         2468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_32746_length_2468_cov_4.539577, whole genome shotgun sequence.
ACCESSION   DAOTBO010000228 DAOTBO010000000
VERSION     DAOTBO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32746_length_2468_cov_4.539577"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>2468
                     /locus_tag="PLE88_13375"
     CDS             14..>2468
                     /locus_tag="PLE88_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77498.1"
                     /translation="MAKRNSGFTVALWACPTAVKGWARFIDFGNGSSSDNILLARYQS
                     TSDLTLEVYNGSVSGGHVRATNAIELNKWQFFAAAVDADGNVKIYKNGQVVKTGKTAA
                     PRQLYRRNLFIGRSNWSADSYYQGAMDDIRLYNYALSDAEITAVYTNSRLDGPIPYSG
                     SNQVSPETVLQFTPAANALRHDIYFGTQYSAVAAADTGSPEYVGRKQAAQYNPPLLDG
                     WREYFWRVDERMIDGTVQTGPVWRFSTVGSIQRQVWTGLSGVNEIAGLTSASSYPANP
                     SLVEHLDTFEIPANWADNYGTRLHGLLVPQTTGSVRFWISGDNEVELWLSTDADPAHA
                     ARIAYIYGTWSNPRSFDQYASQRSALFTLQAGQPYYIMALHKEGDGGDHLAVAWQGPD
                     SPDRSIIDGFWLRPPRDNEWPHFEPALPAALNAPEGAAFQAVMPVRAADPDTAGLNYA
                     KQAGPAWLHIASDGRLWGTPQNGDVGLNSFTIRAGDGRGGFDDLILSVDVPNRFTGSQ
                     GLEDLTGFAHEWLNPDSQSPANLNQAGAVTFDDWKLLAAQWSSAPGSGLAAYWAMDDS
                     IGQIARDSQSHHHAILTHMTEHHWVGGISQNALAFDGIDDYVEAVGYKGITGSASRTC
                     TAWIKTTVPGTNILSWGAAQTGRLWAIALSSDGTLRVQVYGDYIYGTTPLTDGQWHHI
                     AVMLPDDGSPNLTEAVLYVDGLPETSIGGSATCPINTAASADVCIGANVPRTIFYKGV
                     IDEVRLYDRLLSRQEIEKLALRRLQLYLPLDETAGLTAADYSAKNRIGQLFGQPAWKP
                     GMGVRGGAVSFDGIDDYIQI"
BASE COUNT          524 a          723 c          710 g          511 t
ORIGIN      
        1 gctgccttcg ggaatggcca aaagaaacag cggatttacc gttgccttgt gggcctgccc
       61 gacagccgtc aaggggtggg cacggtttat cgacttcggc aacggttctt cttcggacaa
      121 tattctgctg gcccgctacc aaagcaccag cgatttgacg cttgaggtgt acaacggctc
      181 ggtcagcggc ggccacgtgc gggcgaccaa tgccattgaa ttgaacaaat ggcagttctt
      241 tgccgctgcc gtggatgctg acgggaatgt aaaaatctac aaaaacggtc aggtcgtaaa
      301 aaccggcaaa accgccgctc cccgccagct gtaccgccgc aatcttttca tcggccgcag
      361 caactggtcg gccgacagct attaccaagg cgctatggac gacattcgtc tttacaacta
      421 tgccctgtca gatgcggaga ttacggcggt ttataccaac agccgtctgg atgggccgat
      481 tccctacagc ggcagcaatc aggtcagccc cgaaaccgtt ttgcagttta ccccggcggc
      541 caatgccctg cggcatgata tctatttcgg cacgcagtac agcgctgtgg ctgcagccga
      601 taccggcagc cccgaatatg tcggccgaaa gcaggcggcc caatacaatc ccccgcttct
      661 ggacggctgg cgggaatact tctggcgggt ggacgagcgg atgatcgacg gcaccgtaca
      721 gaccggtccg gtatggcggt tttcaaccgt tggaagcatc cagcggcagg tctggaccgg
      781 actgtcagga gtcaatgaaa ttgccggtct gaccagcgcg tccagctatc cggccaatcc
      841 gtcgctggtg gagcatctgg atacattcga aataccggct aactgggcag acaattacgg
      901 aacacgtctg cacggtctgc tggttcccca gacaaccggc agcgtgcgat tctggatctc
      961 cggcgacaac gaagtagagc tgtggctgag caccgatgcc gatccggccc atgccgcacg
     1021 catcgcctat atttacggca catggtcgaa tccccgcagt tttgaccagt atgcttccca
     1081 gcggtcggca ctgtttacac tgcaggcagg ccagccgtat tatataatgg ccctacataa
     1141 ggaaggcgat ggcggcgacc atctggctgt cgcctggcag gggccggaca gtcccgaccg
     1201 cagcattatt gacggcttct ggctgcggcc gccgcgggac aatgaatggc cgcattttga
     1261 accggctctg ccggcggctt tgaatgcgcc ggaaggcgcc gcttttcagg ccgtcatgcc
     1321 tgtccgcgca gccgaccccg atacggccgg cctcaattat gccaaacagg ccggccccgc
     1381 atggctccat attgcttctg acggccggct ttggggtact ccgcaaaacg gcgatgtcgg
     1441 ccttaattcc tttaccattc gtgccggcga cggcaggggc ggcttcgatg atctcatcct
     1501 gtctgttgat gttcccaatc gctttaccgg ttcgcaaggg ctggaagatc tgaccggatt
     1561 tgcacacgag tggctaaatc ccgattccca aagtccggcc aatctgaatc aggccggcgc
     1621 cgttacgttc gatgactgga aactgctggc ggcacaatgg agcagcgcac ccggcagcgg
     1681 tctggcggcc tattgggcga tggatgactc catcggacag attgccagag acagccaatc
     1741 ccatcaccac gccattttga ctcatatgac cgaacaccat tgggtcggcg gcatctccca
     1801 aaatgctctg gcttttgacg gcattgatga ttatgtggaa gcggtcggct ataaaggcat
     1861 taccggctcg gccagccgca cctgtacagc gtggatcaag acaacagtcc ccggcacaaa
     1921 catcctcagc tggggtgcag cacagacagg ccggctgtgg gcgatagccc tcagcagcga
     1981 tggaaccctg cgggtgcagg tttacggcga ttatatttac ggcacaaccc cgctgaccga
     2041 cgggcagtgg catcatatag ctgtgatgct gcccgatgac ggttctccca acctcaccga
     2101 agctgtcttg tatgtcgatg gtctgcccga gacatctatc ggcggttctg caacctgtcc
     2161 tatcaataca gccgcttctg cggatgtctg cattggagcc aatgtccccc gaaccatctt
     2221 ttacaaaggg gtaattgatg aagtccggct ttacgaccgc ctgttgagcc ggcaggaaat
     2281 tgaaaagctc gccctgcgcc ggctgcagct ttatctgccg ctggatgaga cagcaggttt
     2341 gacagccgct gattattcag ccaaaaaccg catcggccaa ttgttcggcc agccggcatg
     2401 gaagccggga atgggcgtta gaggcggggc ggtgtcgttt gacgggatag acgattacat
     2461 ccagatca
//
LOCUS       DAOTBO010000229         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_32906_length_2459_cov_4.096922, whole genome shotgun sequence.
ACCESSION   DAOTBO010000229 DAOTBO010000000
VERSION     DAOTBO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32906_length_2459_cov_4.096922"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLE88_13380"
     CDS             <1..753
                     /locus_tag="PLE88_13380"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOM77499.1"
                     /translation="IERYRVECLRSVLINTHLTEAAYRAGAERYFFSSSACAYNVELQ
                     KDPNVRALKESDAYPAMAERGYGWEKLFSEMVCQEYWHERGLKTAIARFHNVYGPFGT
                     WDGGREKAPAAICRKVIEAKDTGRMDIEIWGDGSQTRSFMYIDDCVLGIDMITHCDKL
                     TAVPINLGSCELISINTLVDLVEQIAGVKLQRRYNLNAPRGVAGRNSDNTFIKQMLSW
                     EPTTPFKTGLTKTYQWIEQQYYDRKAGRRTVS"
     gene            770..1567
                     /locus_tag="PLE88_13385"
     CDS             770..1567
                     /locus_tag="PLE88_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77500.1"
                     /translation="MTSSRHHITDIIGKLQYRLALAGGWIDQPFVSRYDPQPPGSMVV
                     AAVEPQFRWMDRAGICGSTRSIALKLWGGRLPDEPLDVLVRKLYEAENKGKADPSGSQ
                     DMIGIIYPGICRLDYDYAYEGGLFPRHIESINDPDTARWLQEHLYILPVNMRPEGYSP
                     LGRQNLTPEWVRRLGQSGWDCFDAIARRNLAALGQSFNDCMKAWSVLMPDILQHPLLP
                     KDLTALLEYYQSRYAGAMYSGCGGGYLYVASSEPVAGGFQVQIKVAL"
     gene            1564..2376
                     /locus_tag="PLE88_13390"
     CDS             1564..2376
                     /locus_tag="PLE88_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOM77501.1"
                     /translation="MTAQILTAGTFDPMDARRIRFLQEASRCGRLTVVLLEDELVQKL
                     TGAVPKFPLAERLYFIQNIRYTAEVETAASLEHLKDIGCRRRPSEWISPAFEDLSEFR
                     TAAAGCGAGYRLIDEAALAGLPQHDYQTTPSSSRKVIVTGCYDWFHTGHIRFFEEASQ
                     YGDLYVVVGHDKNIELLKGPGHPMFGQEQRAYIVGSIRFVRQALVSTGSGWLDAEPEI
                     RRLKPDCYIVNEDGDKPIKRRFCQSLGIEYIVLKRLPKPGLMRRTSTDLRGF"
BASE COUNT          524 a          706 c          711 g          518 t
ORIGIN      
        1 atcgagcggt atcgcgtgga atgtctccgc agcgtcctga ttaacaccca tctgaccgaa
       61 gccgcctacc gggccggcgc cgaacggtat ttcttttctt cttcggcctg tgcctacaat
      121 gtggaattgc agaaagaccc caacgtgcgg gcactgaagg aatcggatgc ctatccggcg
      181 atggccgagc gcggctacgg ctgggaaaag ctgttttccg aaatggtctg tcaggaatac
      241 tggcacgagc gcggcctcaa aacagccatt gcccgcttcc acaatgttta tgggccgttc
      301 ggcacgtggg acggcggcag ggaaaaggcc ccggcggcca tttgccgcaa agtgattgaa
      361 gccaaagaca ccggccgaat ggacatcgaa atctggggcg acggctccca aacacgcagc
      421 tttatgtata tcgatgactg cgtgctgggc atcgatatga ttacccattg cgacaagctg
      481 accgctgtgc cgatcaacct cggctcctgc gagctgattt ccatcaacac gctggtggat
      541 ttagtcgagc agatcgccgg cgtcaaactc cagcggcgct acaatctcaa tgccccgcgg
      601 ggcgtggccg gccgaaacag cgacaacacc ttcatcaagc agatgctcag ctgggagccg
      661 acaacgccct tcaagaccgg tttgaccaag acctaccagt ggattgagca gcagtactat
      721 gaccgcaagg ccggcagaag gactgtcagc tgacagcagg ggcgcacaga tgaccagcag
      781 cagacatcat ataacggata ttatcggaaa gctgcagtac cggctggcac tggccggcgg
      841 ctggattgac cagccgtttg tatcccgcta tgacccccag ccgcccggct caatggtggt
      901 ggcagccgta gagccgcagt ttcgatggat ggaccgtgcc ggcatctgcg gcagcacgcg
      961 ctcgattgcg ctgaagctgt ggggcggccg cctgcccgat gagccgctcg atgtgctggt
     1021 tcgcaagctc tatgaggcgg aaaacaaagg caaggccgat ccgtccggtt cgcaggatat
     1081 gattgggatt atctatccgg gcatctgccg gctggattac gattatgcct atgagggcgg
     1141 gcttttcccg cggcatatcg aatccatcaa cgaccccgat accgcccgct ggctccaaga
     1201 gcatctttat atcctgccgg tcaatatgcg tcccgaaggc tacagcccgc tgggccggca
     1261 gaatctgacg cccgagtggg tgcgccggct cgggcagtcc ggctgggatt gctttgacgc
     1321 cattgcccgc cgcaatctgg ccgcgctggg gcagtcgttt aacgactgta tgaaggcctg
     1381 gagcgtgctg atgcccgata ttctgcagca cccgctgctg ccgaaggatt tgacggctct
     1441 gctggagtat tatcagtccc gctatgccgg ggcaatgtat tccggctgcg gcggcggcta
     1501 tttgtatgtg gcctcttctg agccggttgc cggcggcttt caggttcaaa tcaaggttgc
     1561 cctatgacag cacagattct caccgccggc acgtttgacc caatggatgc ccgacgcatc
     1621 cgctttcttc aggaagcttc gcgctgcgga cgcctgacgg tggtgctgct ggaagatgag
     1681 cttgtccaga aattgacagg ggcggtaccc aagtttccgc tggccgagcg gctgtatttt
     1741 atccagaata tccgttatac tgcagaggtt gaaacggcag cctctctgga gcatctcaaa
     1801 gatatcggct gccgccgccg tccatcggag tggatttcac cggcctttga ggatttgtca
     1861 gaattccgga cggccgccgc cggctgcggt gccggctatc gtctgataga tgaggccgcg
     1921 ctggctggat tgccccagca cgattaccaa acaactccct cctcatcccg aaaagtgatt
     1981 gttaccggct gttatgactg gtttcatacc ggacatatcc gcttttttga agaggcctcc
     2041 caatatggcg atttgtatgt tgttgtaggc cacgataaga atatcgaact gctcaaaggc
     2101 ccaggccacc cgatgtttgg gcaggagcag cgggcttata tcgtcggttc catccgcttt
     2161 gtccgccaag cgctggtttc caccggcagc ggctggctgg atgccgagcc ggaaatcaga
     2221 cgcctcaagc ccgactgcta cattgtcaat gaagacggcg acaagcccat caagcgccgg
     2281 ttctgccaga gtttaggcat agaatacatc gttctaaagc ggctgccaaa gccgggcctg
     2341 atgcgcagaa ccagcaccga cctgcgcgga ttctaaaaaa gcccattatt ggccgacacg
     2401 cattttttcc gcaaaaaatc agaaaaatta cagacaagtt ttcggccatc tggtatagt
//
LOCUS       DAOTBO010000230         2416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_33616_length_2416_cov_3.860229, whole genome shotgun sequence.
ACCESSION   DAOTBO010000230 DAOTBO010000000
VERSION     DAOTBO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33616_length_2416_cov_3.860229"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLE88_13395"
     CDS             complement(<1..1009)
                     /locus_tag="PLE88_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77502.1"
                     /translation="MAIASMQKVMIVAHRSQTAELLARLQEAGIMQILDAERAMVTKE
                     WPELVVEVKRHRDLEETIERLGRAVDFLKPYAGKEPTSLFAPKIEIGAADYGRIVTQQ
                     DSLAVLEKAEHIRNAMEKLSAESQTLEALLGKLLPWKDLSVRLEELGSLTTATVFTGL
                     IAPQHAEAARAKLEPLAAVVQQVGQTGGMAACTVVCLNEAAADVQKILRSVEFEPVSF
                     EGLSGTAADNIAGIQNRKKEIDSQRAELEKQAAQTAKDRLKLQILYDHTQNLYRRIHT
                     QASAPATDHAIFMEGWVKKKDYPKLEALVRDFDGCDLAPIEPAQGEEIPIEIDNNRAV
                     RP"
     gene            complement(1114..1425)
                     /locus_tag="PLE88_13400"
     CDS             complement(1114..1425)
                     /locus_tag="PLE88_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77503.1"
                     /translation="MELIKQIKDTERQAREIVEKARRDAALLLEESQKRRSVRLRQAQ
                     QRRQEAIDKAVALAEERGKKDAEALVRQGQQDIEALQAACQPKIQQCVDAIIQRLQQV
                     P"
     gene            complement(1571..2263)
                     /locus_tag="PLE88_13405"
     CDS             complement(1571..2263)
                     /locus_tag="PLE88_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOM77504.1"
                     /translation="MTKPNLSSYLRQHLDQIGAKKEPGPYITISRQWGCDGIGLGQIL
                     VERLNQRDSQQRWKLYHKELLAQLAKDTGLTEEILEKERISKPNLLKDFLRGLTKNSI
                     PDGFEVRNKITMMVRTIAFDGYAVIIGQGSTAATGDLANGLSIRVEAREWRIARICLR
                     ENLDKQTAALKIAEIEKQREHLRKIYERQNPREPAFNIVFDNSVFTNEQIADHIILAM
                     EQRNMIPKEETK"
BASE COUNT          499 a          680 c          588 g          649 t
ORIGIN      
        1 agggacgaac agcccggtta ttgtcgattt caatgggaat ttcttcgccc tgtgccggtt
       61 cgataggcgc taggtcgcag ccgtcaaaat cccggaccag cgcttccagc ttggggtaat
      121 ccttcttctt gacccagccc tccataaaaa tggcatggtc cgtagccggt gcacttgcct
      181 gcgtatgaat ccgccgataa agattctgcg tatgatcata aagaatctgc agcttgagcc
      241 ggtctttagc cgtctgggcg gcctgctttt ccagctcggc ccgctggctg tcgatttctt
      301 ttttgcggtt ttgaatgcct gcgatattgt cggcagctgt accggaaagc ccctcaaagg
      361 ataccggctc aaactcgacc gaccgaagga ttttctggac atcagcggcc gcttcattga
      421 gacaaaccac tgtgcaggca gccattccgc cggtttggcc gacctgctgg acaacggctg
      481 ccagcggctc aagcttggcc cgtgcggctt cggcatgctg cggggcaatc aggcctgtga
      541 aaaccgtcgc cgtcgtcaag ctgcccagtt cttccagacg cacggacaaa tccttccacg
      601 gcagcagctt gccaagcagg gcctccagcg tctgggactc tgccgacagc ttttccatgg
      661 cattgcggat atgctcggcc ttttccagca ccgccagcga atcctgctgg gtgacaatcc
      721 tgccgtagtc ggcggcgccg atttcgattt tcggggcaaa cagagaggtc ggctctttgc
      781 cggcataggg tttgaggaaa tcaacagccc tgccgagccg ctcgatggtc tcttccaagt
      841 cgcgatgccg cttgacttcg accaccagct ccggccattc tttggtcacc atcgcccgtt
      901 cggcatccag tatctgcata atgccggcct cctgcagacg tgccagcaac tcggccgtct
      961 ggctgcgatg ggccacaatc atcactttct gcattgaggc gatagccatt tatactctca
     1021 cagataacaa ctctctattt gcctgaagac acacttggag cttgacaaaa cgcctgaccc
     1081 ggggaaaaac agccgtttga gcagaaatct gccttacggc acctgctgca accgctgaat
     1141 aattgcatcg acacattgct gaattttcgg ctgacaggcc gcttgaaggg cttcgatgtc
     1201 ctgctggccc tgcctgacaa gtgcttcggc gtcctttttg ccgcgttctt ctgccagagc
     1261 aaccgcttta tcgatggctt cctgccgccg ctgctgggcc tgacgcagac gaacagaccg
     1321 ccgcttttgg ctttcctcga gcagtaaagc cgcatcccgc cgggcctttt caacgatctc
     1381 tctggcctgt ctttcggtgt cttttatctg cttaatcaat tccattgtaa aattcgcccc
     1441 ataaggtact gactgaaacc cttcaggtca acatttattc gatttaattt taatgttcct
     1501 aaaatcacat ataccagaaa tcaacgtcgt tatccagaaa aatatttcct gatattgtgc
     1561 aaaataatac ttatttagtt tcttccttcg gaatcatatt ccgctgttcc attgccaaga
     1621 taatatggtc agcgatttgc tcattggtga atacggagtt atcaaacacg atattaaagg
     1681 ccggttctct cggattttgg cgttcataaa tcttccgcag atgctctcgc tgcttttcaa
     1741 tctcagcgat tttaagtgcg gccgtctgct tgtccaaatt ttcccgcagg cagatgcggg
     1801 caatgcgcca ttcccgcgct tctacccgta tgctcaagcc gttggccagg tctcccgtgg
     1861 cggcggtgct gccctgtccg ataataaccg catagccgtc aaatgcgatg gttcggacca
     1921 tcattgtaat tttattccgc acttcgaagc cgtcggggat gctgtttttg gtaagtcccc
     1981 gcagaaaatc tttcagcaga ttgggcttgg agatgcgctc tttttcaaga atttcttctg
     2041 tcaggccggt atctttggcc agttgtgcca gaagctcctt gtggtagagc ttccagcgct
     2101 gctggctgtc gcgctggttg agccgctcga ccagaatctg ccccaaaccg atgccgtcac
     2161 agccccactg ccgggagatt gtgatataag gtcccggctc tttcttggca ccgatttgat
     2221 ccaaatgctg ccgcaaatag gatgaaaggt ttggctttgt catcgcttgc ctctcctgca
     2281 aaaatcgact tatgccgaca ttgttaaaat atatttagag cgccgaggcg ctgctttttc
     2341 gatttgctcc ctgaacggtt tactatacca gatggccgaa aacttgtctg taatttttct
     2401 gattttttgc ggaaaa
//
LOCUS       DAOTBO010000231         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_33838_length_2404_cov_4.041720, whole genome shotgun sequence.
ACCESSION   DAOTBO010000231 DAOTBO010000000
VERSION     DAOTBO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33838_length_2404_cov_4.041720"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLE88_13410"
     CDS             complement(<1..239)
                     /locus_tag="PLE88_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77505.1"
                     /translation="MAKHVTAGEKFRFGAQTYNELNGLIEKDRLNGLSMQAKNARGNN
                     DFVLVKNLSGTDVARFGVLGIAGIVFDPQTALPAFT"
     gene            complement(239..1030)
                     /locus_tag="PLE88_13415"
     CDS             complement(239..1030)
                     /locus_tag="PLE88_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77506.1"
                     /translation="MAVTVTEKFQSRDVVRGTNPSAQLNFVIQGTEDYDEALTQLASK
                     APAAFDNLPRLSYGIEPIAEAIWLGNARYGYQSTQTTGESVYQFDTGGGSQHITQSLG
                     TVRRYARPGAVAGNFMGAIGVTADSVEGVDITVPVYSFSEVHYKNRTFVDDNYKATLF
                     ELTGTVNSRNFRKYAPGEVLFLGASGTKRGADDWELVYRFSASPNMSGIVIGDMGGIA
                     KAGWEYLWVQYVDADDSNAQTLIKQPFSVHIEQVYPYKDFDRLRL"
     gene            complement(1030..>2404)
                     /locus_tag="PLE88_13420"
     CDS             complement(1030..>2404)
                     /locus_tag="PLE88_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77507.1"
                     /translation="STEDAKAAEVLNDTMDRLWKVVKMTAFHVGAALAPALQKITDVI
                     TNIAVKINAWIQENHAVIVSIGKIALAVLAGGAALVVLGTAISGLGLAFGKISAVITG
                     VGTVFKMLGSVIAFLVSPIGLVITAIGALGVYLVYTTDVAGKAVDWLGQKFSILKDDA
                     VQAYQGIADAMAAGDIALAAKILWLTLKMEWTRGVNVLEKAWLNFRNFFIRIGYDAFY
                     GMVAAAQTVWHGLEVGWIETMAFFSRCWQGFVSFFAKTWENIKAGAQKAWNWIKSLFD
                     DSFDLETENKMVEDQRQQAIAQIEDEKQRKLAEREAERQSQREQAARMNDATLEEIGR
                     QHAEKYQALDDEYNARMAENTKDLEAARKEWKDSIQRAKDKRAEKELEKPAAVEKANT
                     AWMDVGDVLTEAANKISVTGTFNATAAWGLGTGSAADRTAKATEETARNTKRLLDEAR
                     NNGATFS"
BASE COUNT          546 a          692 c          558 g          608 t
ORIGIN      
        1 gtaaatgccg gaagcgccgt ctgcggatca aacacaattc ccgcaatccc taatacacca
       61 aaacgtgcaa catcagtgcc gctgagattc ttaaccagca caaaatcatt attaccgcgt
      121 gcgtttttgg cctgcatgga tagaccattg agtcggtctt tttcaatcaa accgttcaac
      181 tcattgtacg tttgagcccc aaaccggaat ttctcacctg cggtaacgtg ctttgccatt
      241 acaacctcaa ccggtcaaaa tctttgtagg ggtagacctg ttcaatatgt acggaaaaag
      301 gttgtttgat gagggtctgg gcatttgagt catcagcatc aacatactgg acccacaggt
      361 attcccatcc tgccttggca atccctccca tatcgccaat aacaattcct gacatattcg
      421 ggctggctga aaaccgatag accagttccc aatcatcagc accccgtttg gttccactgg
      481 caccaaggaa gagcacctcc ccaggagcat acttgcgaaa gtttcgactg ttgaccgtcc
      541 cggtcaattc aaataaggta gccttataat tgtcatcgac aaaagttcta tttttatagt
      601 gcacctcact gaagctgtag acgggaacgg taatatcgac accttcaacc gaatcggccg
      661 ttacaccgat ggcccccatg aaattacctg ccacagcacc cggccgtgcg tagcgacgga
      721 cggttcccag gctttgggtg atatgttgac tgccgccgcc ggtgtcgaac tgatagaccg
      781 attctcccgt tgtctgggtg ctttgataac cgtaccgggc attacccagc caaatcgcct
      841 cggcaatcgg ctcgataccg taacttagac gcggcagatt atcgaaagcc gctggtgcct
      901 tactggccag ttgtgtcagc gcctcgtcat aatcctcggt cccctgaatg acaaaattca
      961 attgggccga gggattggtg ccacggacca catcgcggga ttggaatttt tcagttacgg
     1021 taacagccat tatgagaacg ttgctccatt attgcgtgcc tcatccaaga ggcgtttggt
     1081 attgcgggcg gtttcctcgg tggccttcgc ggtccggtcg gcggcgctac cggtgcccag
     1141 cccccaggcg gcggtggcat tgaaggttcc ggtgacgctg attttattgg cggcttccgt
     1201 caaaacatca ccgacatcca tccatgcggt gttggctttt tcgacagcgg ccggtttttc
     1261 cagttccttt tcggcacgtt tatccttggc tcgctggatg gagtccttcc attctttccg
     1321 tgccgcctct aaatctttgg tgttttcggc catgcgggcg ttgtattcat catcgagggc
     1381 ctggtacttt tcggcgtgct ggcgaccaat ctcttctaag gtcgcatcat tcatccgggc
     1441 cgcttgctcc cgctgggact gacgttcggc ctctcgttcg gctaacttac gctgtttttc
     1501 atcctcgatt tgagcgatgg cctgctgacg ctggtcttcg accatcttgt tttccgtttc
     1561 caaatcaaac gagtcatcaa acagggattt aatccagttc cacgcctttt gtgccccggc
     1621 tttgatgttc tcccaggtct tggcaaagaa cgatacaaat ccctgccagc aacggctaaa
     1681 aaaggccatc gtttcaatcc agccgacttc caagccatgc cagacggtct gagcagcggc
     1741 caccatgcca taaaacgcgt catatccgat gcggataaag aaattgcgaa agttcagcca
     1801 tgccttttcc agaacattga ccccgcgggt ccattccatc ttcagtgtca gccataaaat
     1861 cttggctgcg agcgctatgt ctcctgcggc catcgcgtct gcaatgcctt ggtacgcctg
     1921 gacggcatcg tctttcaaaa tggaaaactt ttgacccagc caatcaacgg ctttgcctgc
     1981 cacatcggtt gtatagacca gatacacacc cagggcaccg atagcggtaa taacaagccc
     2041 aatcggtgaa actaaaaatg caattaccga accgagcatc ttaaaaaccg tgcccacgcc
     2101 ggtaatcacc gcactgattt ttccaaaggc aagccccaat ccgctgatcg ccgtacccaa
     2161 caccaccagt gcagcaccac ccgccagaac cgctaatgca attttgccaa tactgacaat
     2221 gactgcatga ttttcttgta tccaagcgtt gattttgacg gcaatgttgg taatgacgtc
     2281 cgtgattttc tgtaaggcag gcgctaatgc cgcaccgaca tgaaaggccg tcatcttaac
     2341 gaccttccag agccggtcca tcgtgtcgtt gagcacctct gccgccttgg cgtcctccgt
     2401 gctc
//
LOCUS       DAOTBO010000232         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_34191_length_2385_cov_4.293133, whole genome shotgun sequence.
ACCESSION   DAOTBO010000232 DAOTBO010000000
VERSION     DAOTBO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34191_length_2385_cov_4.293133"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PLE88_13425"
     CDS             complement(<1..1276)
                     /locus_tag="PLE88_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77508.1"
                     /translation="MKKMIIFLLCISAGSIQAAIRGPLPVDKNTRHLYQFDVESAGIM
                     HDMVRTNPIDLALYNKASLKASASGCGNALNTYDNTAGNNGPFAGRAGDYNLTAAISE
                     FTGADGAFTFEALVRPDAGPAGLLGHMEIISGETDGGPTDRGFQFRIQNNGTTLRFQT
                     LSGSTTAFDAPITYTAGQWYHAAITYNGIPNQAGNLKLYWTPIASGASEAVEVGGPFT
                     MTADLDAADQTKFCVGNELRSYSLADENFEGLIDIVRISSIARAPGDMLLGTLIPWAH
                     NPVPATGSLTIDSQNTSAVQWDTAQIAHVTKHYLYIREAEPNFIDPVIVTDTTDPIAA
                     AVPLTLNPDTVYYWRADASINDSSPSDANTVVGPVWSFKTISVVPSFSSHPADQAVFA
                     GDTAVFSVVLVNSVGVTYQWYKGTQALSDGGTV"
     gene            complement(1363..>2385)
                     /locus_tag="PLE88_13430"
     CDS             complement(1363..>2385)
                     /locus_tag="PLE88_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOM77509.1"
                     /translation="ISTPTLSDEGTYSCLLSVDPALTTDDVRTASAVLVIKRMLAKYD
                     FEQNLSDSSGSNAPSGQVKSVAGLPEPNETAAVSTAPVYVDGIDGKAIYLDGSQFVDF
                     GSGGYPKAGPLNTAGDARGQSYERSGLGHGMEEGTVLCWVKPLTAGAIYTNANSDPDD
                     TLFGLTTASANNARMIVRGENWDGSYQEIGTASGSLNKGNFSLQDGSWHLLAATWQDS
                     TVRVYINGEQVGSATAGFPEKYNPWERSNILGASRSSGTARHILTNFLKGAADKMRIY
                     NYAVSPDEIAAEYAALNELGYRPCANHNFVGSELNYDNSGHSYCRIDLADIAAFAQSW
                     LTNGLY"
BASE COUNT          582 a          599 c          585 g          619 t
ORIGIN      
        1 aaacagtgcc cccgtccgac agcgcctgtg tgcctttata ccactgataa gtgacaccga
       61 cactgttgac caaaacaact gaaaacacgg cagtatcgcc ggcaaatacc gcctgatctg
      121 ccggatgcga cgagaagctc gggaccacac tgattgtttt aaaactccac acagggccaa
      181 caacggtatt ggcatcactg gggctggaat cgttaatgct tgcgtcagct cgccaataat
      241 ataccgtatc gggattgagt gtcagaggga ctgccgcggc aataggatct gttgtgtctg
      301 taacgattac aggatcaata aaattcggtt cagcctcgcg aatgtaaaga taatgtttgg
      361 ttacatgagc aatctgtgca gtatcccatt gaacagcaga agtattttga gaatcaatag
      421 taagactgcc ggtcgccgga acaggattat gagcccacgg aatcaatgtt cctaacagca
      481 tatccccagg agctctggct atgctgctga tgcgaacaat atcgatcagc ccctcgaagt
      541 tttcatcagc aagagaataa cttcgcagtt cattgcccac gcaaaatttg gtttggtctg
      601 ctgcatccaa atcggctgtc attgtaaatg ggccgcctac ttctacggct tcggaagctc
      661 ctgatgcaat cggagtccag taaagcttca ggtttccggc ctgattggga atgccgttgt
      721 aggtaatagc agcgtggtac cattggcctg ccgtataggt aataggcgca tcaaatgctg
      781 ttgtggatcc ggacagagtc tgaaaccgga gtgttgttcc attattttgt attctgaatt
      841 gaaaaccccg gtctgtgggt ccgccgtctg tttcgccgct gataatttcc atatggccca
      901 gaagaccagc cggccctgca tcgggccgaa ccagggcttc aaatgtgaat gctccgtctg
      961 ctccggtaaa ttctgaaatg gcggctgtta gattataatc gccggctcgt ccggcaaaag
     1021 gaccgttatt tccggcggta ttatcatacg tattcagggc gtttccgcat ccggatgcgg
     1081 atgcttttaa agatgctttg ttatagagtg ccaaatcaat cggattggtc cggaccatat
     1141 catgcataat acctgcggat tccacatcga attgatacag atggcgggta ttcttatcga
     1201 caggcagagg tccccgtata gcagcttgga tgctgcccgc acttatgcaa agcagaaaaa
     1261 ttatcatttt tttcattgtt tcaactcctg tttttcaaat tatcaaacaa tgcttgacat
     1321 aatgttttgc ctgacagtct caatcttcaa tccgctggtt gctcagtata atccatttgt
     1381 cagccagctt tgagcaaaag ccgcaatatc ggccaaatca atccggcaat acgaatgacc
     1441 gctgttgtca tagttcaatt cactcccgac aaaattgtga ttggcacacg gtctatagcc
     1501 gagttcattc aaggcggcat attcagcggc gatttcgtca ggcgagacgg cgtagttgta
     1561 tattcgcatt ttatctgccg cacctttgag gaaattggtc aatatatgac gggctgtccc
     1621 agaggaccgc gaagccccta aaatattgct tcgctcccaa ggattgtatt tttcaggaaa
     1681 tcctgctgtt gcagagccca cctgttcgcc gttgatatac acccgcacag tgctgtcctg
     1741 ccacgtggca gcaagcaaat gccaggagcc gtcttgaaga ctgaaattgc ctttattcag
     1801 actgccgctg gcggtgccga tttcctgata agatccgtcc cagttttcac cgcggacaat
     1861 catgcgggca ttgttggcag aggcggtggt taatccgaaa agcgtatcat ccggatctga
     1921 attagcattg gtatagatag cccctgctgt aaggggtttt acccagcaca aaacagtacc
     1981 ttcttccatg ccatggccaa gacctgaccg ttcgtaactt tggccgcgag cgtcaccagc
     2041 ggtattcagt ggaccggcct ttggatatcc gccactgccg aaatcaacaa actggctgcc
     2101 gtccagataa atcgctttgc cgtcaatgcc gtccacatag accggtgctg tagaaacggc
     2161 tgctgtctcg ttgggttcgg gtaaaccggc tactgatttg acttgtcctg acggggcatt
     2221 gctgccgctg ctgtcggaca aattctgctc aaagtcgtat tttgccagca tccgcttgat
     2281 aaccagtacg gccgacgcag tccgtacgtc atcggttgtc agggccgggt cgacactcaa
     2341 caggcaggaa tacgtccctt catcgctcag ggtgggtgta ctaat
//
LOCUS       DAOTBO010000233         2355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_34742_length_2355_cov_5.376522, whole genome shotgun sequence.
ACCESSION   DAOTBO010000233 DAOTBO010000000
VERSION     DAOTBO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34742_length_2355_cov_5.376522"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLE88_13435"
     CDS             complement(<1..616)
                     /locus_tag="PLE88_13435"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77510.1"
                     /translation="MIMRGSWIACAAMCGLAGLMWAQRQTETIDRGLIAVPRSEGGVY
                     LGWRLFESDPSDIAFNLYRTIGGKTAIRLNAKPLVQTTDYSDLAVPLDSDNRWHLCAV
                     IDGKEQPAEALVQLPANPPAKPYISLPFRGNYVCSKVAIADLNGDGKYDFVIKQPAQI
                     SDPGVWRKSEDTCKIEAYLNDGTFLWRRDLGWNIEQGVWWSPMVV"
     gene            complement(665..>2355)
                     /locus_tag="PLE88_13440"
     CDS             complement(665..>2355)
                     /locus_tag="PLE88_13440"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HOM77511.1"
                     /translation="GRVFISWRLLAEDPLGIAFNLYRQTEGKEPVKLNEAPLAKGTNF
                     IDEKPELSLPTGYFIRPVVNGKEQTASKSWIIDPNAPVMPFISIPLKTPERCRPGDAA
                     VGDLDGDGEYEIVLKQEMRPRDNAFNGISGQTKLEAYDLDGTFLWRIDLGKNIREGAH
                     YIPFIVYDLDGDGKAEIACKTADGTVDGRGKVIGNAEADWRDQNGRVLEGPEFLTIFD
                     GRTGAALATTDYIPPRGDIRDWGDNTGNRVDRFLACVAYLDGQRPSLVMCRGYYAKTT
                     LSAWNWRDGKLTHLWIFDSDDGTEGNRAYRGQGNHNLSVGDVDSDGKDEIIYGACAID
                     HDGRGLYSTGMGHGDAMHLSDIDPDRPGLEVFAIHENPRHPNGANLRDAATGRVIWGL
                     ESNDVVRGVTMDIDPRYRGYECWAFGGRLRGLYSCKGQKIADFSPRSCNMGIWWDGDV
                     LRELLDGTTISKWNYQDQTETVLLEARRWDCIRVNGSKSNPSFYADILGDWREEVIWP
                     TRDGSQLRIFVTTIPTDRWMPTLMHDPVYRLSAVWQNVGYNQPTQTGFYLGASD"
BASE COUNT          561 a          719 c          489 g          586 t
ORIGIN      
        1 acacaaccat tggcgaccac cagacgcctt gctcaatatt ccagcccaga tcccgccgcc
       61 atagaaaggt gccgtcattt agatacgctt caatcttgca ggtgtcttcg cttttccgcc
      121 atactcccgg atcgcttatc tgggcaggct gttttatcac aaaatcatat ttcccgtctc
      181 cattaaggtc cgctatcgca accttgctgc aaacataatt gccgcgaaac ggcagcgaaa
      241 tatagggttt ggcaggcgga ttagccggca attgaaccag cgcttcggcc ggctgctctt
      301 tgccatcaat cacagcacat agatgccagc gattgtcact gtccagcggc acagcgagat
      361 cactataatc tgtcgtctgt acaagcggtt ttgcattcag ccgaattgct gtctttccgc
      421 cgattgtgcg atacagatta aatgcaatat ccgaagggtc gctttcgaac agacgccatc
      481 ccagatacac tcctccttcg ctgcggggta cagcaatcag cccccggtcg attgtctcag
      541 tctgacgctg ggcccacatt aatcctgcca atccgcacat ggctgcacat gctatccaac
      601 ttccccgcat tatcatttcg tctttcctaa gctattctgc ttttcatttg acccaatacc
      661 ttgttcaatc cgaagctccc agataaaagc ctgtctgtgt cggctgatta tagcccacat
      721 tctgccaaac agcactgaga cgatagaccg gatcgtgcat cagtgtcggc atccaccggt
      781 cggttggtat tgttgttaca aaaatacgca gttgcgagcc atctcgtgtc ggccagataa
      841 cctcctcccg ccaatctccc agaatatcag catagaaact cggatttgac ttagacccat
      901 tcactcggat gcagtcccac cggcgtgcct ccagcagcac cgtttccgtc tgatcctgat
      961 aattccattt cgagatagtt gtgccatcga gcagctcccg cagaacatct ccatcccacc
     1021 agatgcccat attgcaagaa cgcggcgaaa aatctgcaat tttttgccct ttgcaggaat
     1081 aaagcccccg cagcctgcct ccaaaggccc agcattcata cccgcgatag cgcgggtcaa
     1141 tatccatcgt cacccctcgt accacatcgt ttgactccag cccccagatt accctgcctg
     1201 tggccgcatc gcgcagatta gcgccgttgg gatgcctcgg attttcatga atggcaaaga
     1261 cctccagtcc cggtctgtca ggatcaatat ccgatagatg catggcatcg ccgtgtccca
     1321 taccggtgga atataagccc ctgccgtcat ggtcaatagc acatgccccg taaatgattt
     1381 catcttttcc gtcgctgtca acatccccta cgctgagatt atgattgccc tgaccgcgat
     1441 aagctcgatt gccctccgtt ccgtcatcgc tgtcaaatat ccacaaatgc gtcagtttcc
     1501 cgtcgcgcca gttccaagca gataatgtcg tttttgcata atatccgcgg cacatcacca
     1561 aactgggacg ctgaccgtct aaatatgcaa cacaagccag aaagcgatcg acgcgattgc
     1621 cggtgttatc gccccaatcc cgaatatccc ctcgcggcgg aatataatcg gtcgttgcca
     1681 atgccgcacc tgtgcgccca tcaaaaatcg ttaaaaactc cggtccctcc agcacacgcc
     1741 cgttctgatc gcgccaatct gcctctgcat ttccgataac tttgcccctg ccgtcaaccg
     1801 taccatcagc ggtcttacag gcaatctctg ctttaccgtc gccatccaaa tcataaacaa
     1861 taaatggaat ataatgtgct ccctcacgaa tattcttgcc taaatcaatc cgccaaagaa
     1921 aagtgccgtc aagatcataa gcctccagtt tggtctggcc gctgatgcca ttgaaggcat
     1981 tatcccgagg cctcatttcc tgtttgagga caatttcata ttcgccatct ccatccagat
     2041 ccccgactgc cgcatcgccg gggcggcagc gttccggagt ctttagcgga atagaaataa
     2101 acggcataac cggcgcattc gggtcaataa tccaagactt gcttgctgtc tgttccttcc
     2161 cattaacgac aggacgtata aaatagcccg tcggcaatga caattcgggc ttttcatcga
     2221 taaaattagt ccctttggca aggggggctt cattcagctt gaccggctct ttgccttctg
     2281 tctgacgata cagattaaag gcaattccca atggatcttc tgccagcagc cgccagctta
     2341 taaataccct tccat
//
LOCUS       DAOTBO010000234         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_34969_length_2342_cov_3.122431, whole genome shotgun sequence.
ACCESSION   DAOTBO010000234 DAOTBO010000000
VERSION     DAOTBO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34969_length_2342_cov_3.122431"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLE88_13445"
     CDS             complement(<1..255)
                     /locus_tag="PLE88_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77512.1"
                     /translation="MLNKGLDWLEQRLLGFCSSPVEYRRDSNTQTIDAVFGKTDIEVE
                     DQTGISVSSFVWDFLIDAQTLGFEPDVGDIIVINSRLFEVM"
     gene            complement(256..597)
                     /locus_tag="PLE88_13450"
     CDS             complement(256..597)
                     /locus_tag="PLE88_13450"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOM77513.1"
                     /translation="MAIAQFVQQGNSIDHTPSGAVTAGDVIVQGDLIGIAQVDCVANQ
                     IGALAVTGVFDIDKATGVGTGIAVGTKIYWDVADQQAKADDESGANKYLGKTIAAAGD
                     DDAKVRVRLEQ"
     gene            complement(654..>2342)
                     /locus_tag="PLE88_13455"
     CDS             complement(654..>2342)
                     /locus_tag="PLE88_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77514.1"
                     /translation="TSWARDVIKSGKNGFPWQASIGAEIIQAEFIPNGNQIQVNGRTF
                     DGPLYVIRKSILKEISFVDNGADTNTSAQVAAQSGEQKQMNDETKNTDLNANEVKSEA
                     AKEDVQASETHTCHCKNKTPEKIEASAASDPVADMRKSIAAETNRIQAIRTVCNGKHT
                     DIEAKAIEEGWDVIKCELEVLRASRPKAPAVHTQKTVHTPHIFEAVALMAAGIGNTRL
                     EAMYDVPTLEAADKLRGIGIQEFCEQVVGQQLPRFRRDASGWLQAAFSTTSLPGILSN
                     IANKMLLEGYNYIEDAWRQICKIASVNDFKEHSRYRMTGAFKFEQVGPDGELKHGKLD
                     EMKYGQKADTHGIMFALTRQMIINDDMGAFTDIPRQIGMGAAEAIADAVWGLLLSNPN
                     SFFSAGNKNYLDGADTALSVDSLTAAEVLFSGQTKPNGRPLGIGASILLVPTALKVAA
                     QLLMSSVLLNETTTANKGKPANNPHVGKFGVVSSAYLANASFAGASSKAWYLFADPNR
                     LPALEVAFLNGVDRPTVEKTDADFNTLGIQFRGYIDFGVREQDYRGAIKLKGEV"
BASE COUNT          486 a          654 c          603 g          599 t
ORIGIN      
        1 catcacttca aataaccggc tgttaataac gatgatgtca cccacatcag gttcaaatcc
       61 cagcgtttgt gcgtcaatta gaaaatccca cacaaaactg ctgactgaga tgccggtttg
      121 gtcctcgact tcgatgtctg ttttgccgaa taccgcatcg atggtttgtg tattgctatc
      181 cctgcggtat tccaccgggc tggaacagaa gccgagtagc ctctgctcca gccaatctaa
      241 acctttattg agcatctatt gctccagtcg aaccctgacc ttggcatcat catcgccagc
      301 agcggcgatg gttttgccca ggtacttgtt cgcgccggat tcgtcatcgg ccttggcctg
      361 ctggtcagcg acgtcccagt agatttttgt accgactgcg atgcccgttc ccacgccggt
      421 cgctttgtcg atgtcgaaga caccggtgac cgccagcgca ccaatctgat tagcaacaca
      481 gtccacctgt gcaataccga ttagatcgcc ctggacaatg acatcaccgg ccgtgaccgc
      541 gccggacgga gtgtgatcaa ttgaattccc ctgttgaaca aattgcgcaa ttgccataaa
      601 atatcctctc tgaaattaaa ggttatcatc cacacaagct tgagggatga ggtttacacc
      661 tcacccttga gcttgattgc tccacggtag tcctgttccc ttaccccgaa atcgatatag
      721 ccacggaact gaatccccag tgtgttgaag tccgcatcgg tcttctcgac ggtcggacgg
      781 tccacaccat tgaggaacgc aacctccaac gcgggcagac ggttcgggtc ggcgaataga
      841 taccacgcct tgctgctggc cccggcaaac gaggcgtttg ccagatacgc actggaaacg
      901 acaccgaact tgccgacatg cggattattg gcgggtttgc ccttgttggc cgtggtggtt
      961 tcatttagca gcaccgaact catcaagagt tgtgcagcga ccttcagtgc cgtcggcacc
     1021 agcagaatgg aagcaccaat tcccaggggc cgaccgttgg gtttggtctg tccgctgaac
     1081 aagacctctg cggccgtcag cgaatccacg ctcagcgctg tatcagcacc atccagatag
     1141 tttttgttac ccgcagagaa gaagctattg ggattactca ggagcaggcc ccagaccgca
     1201 tcggcaatcg cttctgccgc gcccatccca atctgacgcg ggatgtccgt aaaggcaccc
     1261 atgtcatcat tgataatcat ctggcgggtc agggcaaaca tgatgccgtg ggtgtcagcc
     1321 ttctggccat acttcatctc atcgagcttg ccgtgcttga gttcgccatc cggaccgacc
     1381 tgttcgaact taaacgcacc ggtcatgcgg taacggctgt gttccttaaa gtcgttgaca
     1441 gaagcgattt tgcagatttg acgccaggca tcctcaatat agttatagcc ttccagcagc
     1501 atcttgttgg cgatgttgct taaaatgccc ggcagtgagg tggtactgaa tgccgcctgc
     1561 agccatccgg aggcatctcg tcggaaccgc ggcagctgct gaccgacgac ctgctcacag
     1621 aattcctgga taccaatgcc acgaagtttg tcggcagcct ccaatgtggg tacatcgtac
     1681 attgcctcca gccgggtatt gccgattccg gcggccatga gggcgactgc ttcgaagata
     1741 tgaggcgtgt gaaccgtttt ttgcgtgtgt acggccggtg ccttcggccg cgaggcacgc
     1801 aatacctcca gttcacactt gattacatcc cagccttctt cgatggcctt ggcctcgatg
     1861 tcggtgtgct taccgttgca gacggtacgg atggcttgga tacggttggt ttcggcagca
     1921 atacttttac gcatgtccgc caccggatcg gacgcagccg aagcctcgat tttttccggc
     1981 gttttgtttt tgcagtgaca ggtgtgcgtt tcggatgcct ggacatcttc ttttgccgct
     2041 tcgcttttga cctcattggc gttgaggtcg gtattttttg tttcatcatt catctgtttt
     2101 tgttctcctg attgtgccgc tacctgagcg gacgtgttgg tgtctgcgcc gttatccacg
     2161 aaactgatct ccttaagaat ggattttcga atgacatata aaggaccgtc aaaagtcctg
     2221 ccgttaactt gaatctgatt gccgttggga atgaactcgg cctggatgat ttcagcgccg
     2281 atgctggcct gccaggggaa accgtttttg ccgctcttga tcacatcgcg tgcccagctg
     2341 gt
//
LOCUS       DAOTBO010000235         2331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_35173_length_2331_cov_5.717926, whole genome shotgun sequence.
ACCESSION   DAOTBO010000235 DAOTBO010000000
VERSION     DAOTBO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35173_length_2331_cov_5.717926"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2331)
                     /locus_tag="PLE88_13460"
     CDS             complement(<1..>2331)
                     /locus_tag="PLE88_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77515.1"
                     /translation="LEALYRQTEQRREQIASQVKIETPDPYINAAAAALCAAADALWD
                     EPQGSVMHGAAAWRAPYAGWRGPYANDAFGWHDRSKRHLTYWAGRQNTKPPADTPMGA
                     DAQFNLARSHTAKQTSGDIGGKHYDMNLVYIDTLIRHLLWTGDIAFAEQLWPVIERHL
                     AWEQRLFRRTFGPDNLPLYEAYAAIWASDDLQYHGGGVTYASAYNYYHNRMIARLAKR
                     LGKDPSPYEKEAELILKAMRSCLWLQECGWYGEWKDLLGLQLVHPNAGLWTFYHTIDS
                     EAASPIEAWQMSRFTDTQIAHIPVYGDAIPKGHYFVLPTTSWMPYAWSTNNTAMAETA
                     HAALAYWQTGRADKAFSLFKGCILDSMYMGTCPGNAQMTSWYDAVRGEAQRDFGDSVG
                     ICARAVVEGLFGIRPDALAGELKIQPGFPSDWDNAAIQHPDLEFSFRRAGMKETYLVR
                     PKFSGPMPLRLTVKARYADIETAAVNGTEARWNCLQDAVDCPQIEICWPAAERYEVEI
                     VWKGQKPARPQAAAIQAQQTRMQAIFDGAMVKEVLDPQQVLSGVEVKDDFFRASPAGL
                     TGHRTVFARLEQGPMVWLEPVMFELRPPLEIIASSVQPPEGVCFRIQNNTPKMIDVPA
                     VIRAGRQSQKRPLQIASQGISEEIVLKASDWQLLAGSNRITVELADGRTVEGIVVNWK
                     ISLQNQPLQWEVINLKPYYNDHLSRIFQNNYLSPRSPYCSLAIPKQGIGSWCDFARTF
                     EVDDSGLIAAGRDSEGRFVLPQGICFAVPQNRNAQNV"
BASE COUNT          530 a          734 c          546 g          521 t
ORIGIN      
        1 aacgttctgt gcattccggt tctgcggcac cgcaaagcaa atcccctgcg gcagcacaaa
       61 acgcccttcg ctgtcccgcc ctgccgcgat caagccggaa tcgtcaactt caaatgttct
      121 ggcaaaatcg caccagcttc caataccctg tttggggata gccagcgagc agtaaggcga
      181 gcggggagaa agatagttgt tctgaaaaat ccggctcaaa tggtcattgt aataaggctt
      241 tagatttatc acttcccact gcagcggctg attctgcaaa ctgattttcc agtttacaac
      301 aatcccttcg accgtccttc cgtcagccag ttcaaccgta atccgatttg aaccagccag
      361 cagctgccag tccgatgcct tcaatacaat ttcttccgat attccctgtg atgctatctg
      421 cagcggacgc ttctggcttt gcctgccggc acgaataacc gccggcacat caatcatttt
      481 cggagtatta ttctgaatcc ggaaacacac tccctccggc ggctggacag atgaagcaat
      541 tatttccaaa gggggacgca gctcaaacat caccggttcc agccagacca tcggtccctg
      601 ttccagccgg gcaaaaaccg tccgatgccc tgtcagtccc gctggcgaag cccgaaagaa
      661 gtcatctttg acctctaccc cgctcaagac ctgctgcgga tcaagcactt ccttgaccat
      721 agccccgtcg aaaatcgcct gcattcgcgt ctgttgggcc tgaatggcag ccgcttgagg
      781 acgtgccggc ttttggcctt tccacacgat ttcaacttca taccgttctg ccgccggcca
      841 gcagatttca atctgcggac aatccacagc atcctgaaga caattccatc gggcctcggt
      901 tccattgacc gccgctgtct caatgtccgc atagcgcgct ttgactgtca gccgcagcgg
      961 catcggtcct gaaaacttcg ggcggacaag atatgtttcc ttcattcctg cacggcggaa
     1021 gctgaattcc aaatccggat gctgtatggc cgcattgtcc caatcgctcg gaaagcccgg
     1081 ctggattttc agttcccccg ccagcgcatc cggccgaatg ccaaaaagcc cttccacaac
     1141 cgcacgggca catatcccga cagaatcccc aaaatcccgc tgggcctcgc cccggacggc
     1201 atcgtaccag cttgtcatct gcgcattgcc cggacaggtt cccatataca tactgtccag
     1261 aatgcagccc ttaaacagcg aaaaggcctt atccgctcgc cccgtctgcc aataagccag
     1321 tgccgcatga gccgtttcag ccattgcggt attattggtt gaccacgcat aaggcatcca
     1381 gctggtcgtc ggcagcacaa aataatgccc cttgggtatc gcatccccat aaaccggaat
     1441 atgggctatc tgcgtatccg taaatcggct catctgccaa gcctctatcg gactggcagc
     1501 ctctgaatca atcgtatgat aaaatgtcca caagccggca ttggggtgga caagctgcaa
     1561 tcccagcaga tccttccatt cgccgtacca gccgcactcc tgcagccaca gacagctgcg
     1621 catggctttc agaatcagct cggcttcttt ctcataaggg cttggatctt tgcccagccg
     1681 cttggccaag cgggcgatca tgcgattatg gtaataatta tacgccgatg cataagtaac
     1741 cccgccccca tgatactgca aatcatcgct ggcccagatt gccgcatagg cctcatacaa
     1801 cggcagatta tccggcccaa aggtccgccg gaagagacgt tgctcccatg ctaaatgacg
     1861 ttcaatgacc ggccagagct gttcagcaaa agcaatatcc cccgtccata ataaatgccg
     1921 tatcagagta tcaatataca ccagattcat atcgtaatgt ttgccgccga tatcgccgct
     1981 ggtctgttta gccgtatgac tgcgtgccag attaaattgg gcatccgccc ccatcggcgt
     2041 atcggcaggg ggtttggtat tctgccgacc ggcccagtat gtcaaatgcc ttttggaacg
     2101 gtcgtgccag ccgaaggcat cattggcata cggcccgcgc cagcccgcat agggcgcccg
     2161 ccatgcagcc gcaccatgca tcaccgaacc ttgcggctca tcccaaagag catccgccgc
     2221 cgcgcacagc gctgctgcgg ccgcattgat atacggatcc ggcgtctcta tcttaacctg
     2281 tgaggcaatc tgttcccgcc gctgctcggt ttgccggtat aatgcttcca g
//
LOCUS       DAOTBO010000236         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_35871_length_2293_cov_5.176944, whole genome shotgun sequence.
ACCESSION   DAOTBO010000236 DAOTBO010000000
VERSION     DAOTBO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35871_length_2293_cov_5.176944"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /gene="feoB"
                     /locus_tag="PLE88_13465"
     CDS             complement(<1..1251)
                     /gene="feoB"
                     /locus_tag="PLE88_13465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOM77516.1"
                     /translation="MRAENDVNPSNPKQTLTVALAGNPNSGKTTLFNALTGARHHVAN
                     YPGVTVETMEGTVRHKGIEIRLVDLPGTYSLTAYSEEELLARHFIVEQHPDVVVDVLD
                     ASNLERNLYLAAQLIEMQVPLVLAFNMSDVAHSRGLEFDLPQMEILLGARIVPMTATR
                     GEGIEQLLDAVVQTAAQENRRIVAISYGREIEQELDGIEEVLKPYASALPRRYPLRWV
                     ALKLLEGDQEVQGWISQPDVCGRVEAAHQRLRTIFRDHPEITVAERRYGFISGACQET
                     IKSTVQIRHDFSDWIDTVLTHRYLGLPIFAVLMFGVFYLTFRVGAWPMGWLERFFVWL
                     GVQITQHWPLESYGWLRSLVVDGVIGGVGGVLIFLPNILLLFLGIAFLEDTGYMARAA
                     FVMDKFFHLFGLHGRSFIPLMLATG"
     gene            complement(1256..1513)
                     /locus_tag="PLE88_13470"
     CDS             complement(1256..1513)
                     /locus_tag="PLE88_13470"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOM77517.1"
                     /translation="MIRKSMTEHIPVRPLSDFPAGSRIRIVDIEAGRGLKNRLASLGL
                     LPDAPIGVIRNDGAGQVIVSVKNSKIILGRGMSHKVFCVQQ"
     gene            complement(1503..1961)
                     /locus_tag="PLE88_13475"
     CDS             complement(1503..1961)
                     /locus_tag="PLE88_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOM77518.1"
                     /translation="MAVRSSDKLSASLEDYLEAIYNLSGRQNVARSKDIAQLLDVSRA
                     SVTGALKALSDKQMIHYQPYGYVVLTEKGRLAARRIAGRHAVLVQFFERVLGADSATA
                     QEAACRTEHTLGTDIADRLTAFIEFVTSRQDNGQDIAQQFQHHWDASNDS"
BASE COUNT          557 a          723 c          543 g          470 t
ORIGIN      
        1 gccggtcgct aacataagcg gaataaaact ccgaccgtgc aaaccgaata aatgaaagaa
       61 tttgtccatc acgaaagccg cccgagccat atatccggta tcttccagaa aggcaattcc
      121 cagaaacagc agcagaatat tgggcagaaa tatcagcact cccccgacgc cgccgatgac
      181 cccgtccacc accagcgacc gaagccaccc gtacgactcc agcggccagt gctgggtgat
      241 ctgaactccc agccagacaa aaaaccgctc cagccagccc atcggccaag cacccacccg
      301 aaatgtcaga taaaacaccc caaacatcag aacggcaaaa atgggcagtc ccagatagcg
      361 gtgcgtcagt acggtatcaa tccaatcgct gaaatcgtgg cgaatctgaa ccgtgctttt
      421 tattgtctcc tgacaggcgc cggaaataaa tccgtaccgc cgctcggcca cggtaatttc
      481 cggatggtcc cggaaaatgg tgcgcagccg ctggtgagcg gcttctacac ggccgcagac
      541 atccggctgg ctgatccagc cctgcacttc ctgatcgcct tccagcagct tcagtgcaac
      601 ccaccgcagc ggataccgcc gcggcagagc cgatgcatag ggcttcagca cctcttcgat
      661 gccgtccagc tcctgctcga tttcccggcc gtaggaaatc gctacaatcc gccgattctc
      721 ctgtgcggcc gtctgcacaa ccgcatccag aagctgctca atcccttccc cccgcgtcgc
      781 cgtcatcggc acaatccgtg cccccagcag aatctccatc tgcggcaaat caaactccag
      841 accccggctg tgggcaacat cgctcatatt aaacgccagt accagcggca cctgcatctc
      901 gataagctgg gcggccagat acagattccg ctccagattg gatgcatcca acacatccac
      961 aaccacatcc ggatgctgct cgacaatgaa atgccgcgcc agcagctcct cttccgaata
     1021 ggccgtcaga ctgtaggtcc ccggcaaatc taccagccgt atctcgatgc ccttgtgccg
     1081 gactgtccct tccatcgtct cgaccgttac gccggggtaa ttggccacgt gatgccgcgc
     1141 accggtcagc gcgttaaaaa gggtggtttt gccgctgttg ggattgcccg ccaaggcaac
     1201 cgtgagcgtc tgcttaggat tggaaggatt cacatcgttc tccgctctca agccgctatt
     1261 gctgaacaca gaagacctta tggctcattc cgcggcccag aatgatcttg gagtttttga
     1321 cgctgacaat cacctgaccg gccccgtcgt tgcggattac gccgatgggg gcatccggaa
     1381 ggagccccag cgatgccagc cggtttttca ggccccggcc ggcctcaatg tccacaatgc
     1441 gaatccggct accggccgga aaatcgctca gcggacgtac cggaatgtgc tcggtcatac
     1501 tcttacgaat cattcgacgc atcccaatgg tgctgaaact gctgggcaat atcctgtcca
     1561 ttatcctgcc ggctggttac aaactcgata aacgccgtca gccgatcggc gatatccgtt
     1621 cccaatgtat gttcagtccg gcaggcggct tcctgtgcgg tcgcactgtc ggcgcccagc
     1681 acccgctcaa aaaactgcac caataccgca tgccgtcccg caatccggcg tgccgcaaga
     1741 cgcccctttt ccgtcagaac cacataccca tacggctgat aatgaatcat ctgcttatcc
     1801 gacaaggcct tcagggcccc ggtaacagag gcacgcgaca catccagaag ctgcgctata
     1861 tccttgctgc gggcaacatt ctgccggccc gaaaggttgt atatcgcctc cagatagtcc
     1921 tctaaagacg cactcaattt gtcactggaa cgaactgcca tgtctgtctc ggatttaact
     1981 actcttaata tagttagtct tgcctaactt ttcaagcaga attttcaaaa tttctcaatt
     2041 gaccgttttt agtccaatac cggtcaaatc agcccaaagg catccagccc gatggcgatt
     2101 ttgctttact caaaagccgg tttacccgat aatataaccg ccaccagcaa atccaaacca
     2161 gagaataaag gatggtacaa tgtgcggaat agtagcatac ttgggtacga aatcggctca
     2221 gccgatactg atagaaggtc taaagcgtct tgaatacagg ggttatgatt cagccggcgt
     2281 agccctgctg aag
//
LOCUS       DAOTBO010000237         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_38665_length_2162_cov_3.932131, whole genome shotgun sequence.
ACCESSION   DAOTBO010000237 DAOTBO010000000
VERSION     DAOTBO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38665_length_2162_cov_3.932131"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..536
                     /gene="lptE"
                     /locus_tag="PLE88_13480"
     CDS             36..536
                     /gene="lptE"
                     /locus_tag="PLE88_13480"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOM77519.1"
                     /translation="MKNIRFVFLVLASVLLVGGCGGYQNSWLYPQTVRTVYVEMFDTT
                     SFRRGHEYALTDAVCKRIESQTPYKIVSNRDTADSVLSGRLGIGTGVLATDRYTARAL
                     EKETLVTVTVTWKDLRTGKLLIDSKEVYASSSFSEQMGQDFEYAIDRAVNKAAVGVVE
                     LMETPW"
     gene            711..>2162
                     /gene="ftsH"
                     /locus_tag="PLE88_13485"
     CDS             711..>2162
                     /gene="ftsH"
                     /locus_tag="PLE88_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOM77520.1"
                     /translation="MTKKPMVPKKKIRLPNPENPAPKYGHGPFSWLLGGLILMTALMM
                     LTRMTRVETLTFSQFEDCVRQRFVQSVVFNESARTIKGQLHPNAVKLVGSGRTAAFEV
                     HYAPELIRSDYAEWLKSNGVAEIDAAADSVWMPILVNLLPILVIAALIYFIFVRNIRA
                     GGGMLMNFGRSKHRIQGKGTKVTFDNVAGIEEAKDEVAEIIEFLKNPKKFKKLGGRIP
                     RGVLLVGPPGCGKTLLAKAIAGEADVPFFSIAGSDFVEMFVGVGASRVRDLFKQAKEN
                     SPCIIFLDEIDAIGKKRGPGFVSGGHDEREQTLNAILVEMDGFDTDDQVIVIAATNRG
                     DILDNALTRPGRFDRQVVVPLPDLKGRMKILQIHARKIRYAPNVDFERLARGTPMFSG
                     AELEALINEAAISASMQDKDYVEMSDFEEARDKVRWGRAKRSREIDENDRRLTAYHEA
                     GHATVQSMLADADPLHKVSIIPRGPMGGATFALP"
BASE COUNT          475 a          592 c          668 g          427 t
ORIGIN      
        1 gaaattgatg cgttgctgta aagggattcg aacaggtgaa aaacatacgc tttgtctttc
       61 ttgtcctcgc ttcggtgctg ctggtcggcg gctgcggggg ctatcagaat agctggctct
      121 acccgcagac ggttcggacg gtctatgtgg aaatgttcga cacgaccagt ttccgccgcg
      181 ggcatgagta cgccctgacc gatgccgtct gcaagcgcat cgagtcgcag acgccgtata
      241 aaatcgtctc caatcgggat accgccgact cggtgctcag cggacggctg ggcatcggca
      301 ccggcgtctt ggccaccgat cgctataccg cccgggcgct ggaaaaggaa acgctggtga
      361 cggttaccgt aacatggaag gacctgcgca cggggaaact gctgattgac agcaaagaag
      421 tgtatgcttc cagcagcttt tccgagcaga tggggcagga ttttgagtat gccatcgacc
      481 gtgccgtgaa caaagctgcc gtcggcgtcg ttgaattgat ggagacgccg tggtaaaatg
      541 cacgtgagga atccggcggg ccagcgcttt ggaataatcc tgacggggca aaggcctgca
      601 ggctgtaatt tcgatggttt tatcgatatg gtttagaggg tcatttttaa gccggccctc
      661 gatttttttg atatcccgat taaacagcag cacaacgaat aaagaacaga atgacaaaaa
      721 aaccaatggt tccaaaaaag aaaatccggc ttccaaaccc cgaaaatccg gcgccgaaat
      781 acggccacgg gccgttcagc tggctgctgg gcggcttgat tttgatgacc gccctgatga
      841 tgctgacgcg gatgacgcgg gtcgaaacgc tgaccttctc gcagtttgaa gactgcgtca
      901 ggcagcggtt tgtgcagtcg gtagtgttca acgagtctgc ccgcaccatc aaaggccagc
      961 ttcaccccaa cgccgtcaag ctggtcggca gcggccgaac cgcggctttt gaagtgcatt
     1021 atgcgccgga gctgattcgc tccgactacg ccgagtggct taaaagcaac ggcgttgccg
     1081 aaatcgatgc cgccgccgac agcgtctgga tgccgattct ggtcaatctg ctgccgatac
     1141 tggtcattgc ggcgctgatt tactttatct ttgtgcgcaa catccgcgcc ggcggcggga
     1201 tgctgatgaa cttcggccgc agcaagcacc gcattcaggg caagggcacc aaggtgacct
     1261 ttgacaatgt tgccggcatt gaagaggcca aagacgaggt ggccgaaatc atcgaatttc
     1321 tcaagaaccc caagaagttc aagaagctgg gcggccgcat tccgcgcggc gttctgctgg
     1381 tcggcccgcc cggctgcggc aaaaccctgc tggccaaggc gattgccgga gaggcggatg
     1441 tgccgttctt cagcattgcc ggaagcgatt ttgtggagat gtttgtcggc gtcggcgcca
     1501 gccgtgtgcg cgacctgttc aagcaggcca aggaaaattc gccctgcatt atctttctgg
     1561 atgaaatcga tgccatcggc aagaagcgcg gccccggatt tgtcagcggc ggccacgacg
     1621 agcgcgagca gacgctcaat gccattctgg tcgagatgga cggctttgac accgacgatc
     1681 aggtgattgt gatcgccgcc accaaccgcg gcgatattct cgacaatgcc ctgacccgtc
     1741 ccgggcggtt tgaccggcag gtggtggtgc cgctgccgga tttgaagggg cggatgaaaa
     1801 tcctgcagat tcacgcccgc aaaatacgct atgcgccgaa tgtggatttc gaacggctgg
     1861 cgcggggcac gccgatgttc agcggtgccg aactggaagc gctgattaac gaagcggcta
     1921 tcagcgccag catgcaggac aaggattatg tggagatgtc cgacttcgaa gaagcccgcg
     1981 acaaggtgcg gtgggggcgg gccaaacgca gccgcgaaat cgacgaaaac gaccgccggc
     2041 tgacggccta ccacgaggcc ggccatgcaa cagtgcagtc gatgctggcg gatgccgacc
     2101 cgctacacaa ggtcagcatc attccccgcg ggccgatggg cggggcgacc tttgccctgc
     2161 cg
//
LOCUS       DAOTBO010000238         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_38716_length_2160_cov_3.885986, whole genome shotgun sequence.
ACCESSION   DAOTBO010000238 DAOTBO010000000
VERSION     DAOTBO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38716_length_2160_cov_3.885986"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLE88_13490"
     CDS             <1..325
                     /locus_tag="PLE88_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOM77521.1"
                     /translation="VKIIREHLLDKRPLSEVCEQYGIAPTQFYQWQKLFFENGAAAFE
                     PKKAGREYELENKVKQLDKKLIKKDEVIAELVEHNIDLKKSMARSKRWMGRAGCPGRS
                     GGLCT"
     gene            309..950
                     /locus_tag="PLE88_13495"
     CDS             309..950
                     /locus_tag="PLE88_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOM77522.1"
                     /translation="MDYVRKWSDKTEIAATAMVNWIGITRSKFYDWQSRYGRVNEHNG
                     WIPRDFWLTNAEKQAIIAYYVAHPLEGYRRLCYMMMDDNIVAVSPASVYRVLSTAGLL
                     QRFNGNPSKKGDGFDQPLKPHEHWHIDISYINICGTFYYLCSILDGFSRYIVHWDIRE
                     SMTEAEVELILQHAREKFPDARPRIISDNGPGLKRFLPPEKPNSMLHVRHVKP"
     gene            1414..2103
                     /locus_tag="PLE88_13500"
     CDS             1414..2103
                     /locus_tag="PLE88_13500"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOM77523.1"
                     /translation="MDRRNFLAQSIVAGGTVALGSVTQTETKSEEKIKIIGVSCSPRK
                     GKTTAAAVQAALDAARETDSRIETELIDLGGMKIAGWSGGAKPDSPQIPFDDYEVEVE
                     PSFTAPNLGGLIIGSPVYFRSMSALCKAFLERLYTMRTPTMRLADKPVGALAVGSFRN
                     GGQELVIEQIITAMLCYEPLIVGGKPNAHQGATLWNNYNDYITKDEFGIKTAKQLGIR
                     VAEAALKIKRV"
BASE COUNT          598 a          481 c          586 g          495 t
ORIGIN      
        1 ggtaaagatt attcgggaac atcttcttga caagcggcct ttgtcagagg tgtgcgagca
       61 gtacggcatt gccccgaccc agttttacca gtggcagaag ctgttttttg aaaacggagc
      121 cgcggcattt gagcctaaaa aggccggccg ggagtatgaa cttgagaaca aagtcaagca
      181 gctcgacaaa aagctgatca aaaaggatga agtcatcgcc gaattggtcg agcataacat
      241 cgacttaaaa aaaagcatgg ccaggtctaa aagatggatg ggcagagccg gatgtccggg
      301 acgaagtggt ggattatgta cgtaaatggt cggataagac ggaaattgca gcaacggcta
      361 tggtgaactg gatcggtata actcgcagta agttttacga ctggcagagc cgctatggca
      421 gggtcaatga gcacaacggg tggatccccc gtgacttctg gctcaccaac gccgaaaaac
      481 aggcaatcat cgcctattat gttgctcatc cgctggaagg ctatcggcgg ctgtgttata
      541 tgatgatgga cgacaatatt gtcgcggtca gcccggccag tgtctatcgt gtgttgtcaa
      601 cggcggggct gctgcaacgc ttcaacggca atccatcgaa aaaaggggat ggcttcgatc
      661 agcccctgaa accgcatgaa cactggcata tcgacatttc gtacatcaac atctgcggga
      721 cattttacta cctgtgttcc attctggacg gctttagccg ctacatcgtg cattgggata
      781 tccgggagtc catgacggaa gcggaggttg aactgattct ccagcatgcc cgtgagaaat
      841 tccctgatgc tcgaccgcgg attatctcgg acaacggccc cgggctgaag cgattcttgc
      901 cgcccgagaa gccaaactcg atgctgcacg tcaggcacgt aaagccatga ggaaggcatc
      961 gtaatgaaaa gacatttgac agacattgcg aaaatcctga taatgcggct ggctggggaa
     1021 acggacgcag gctctgcaga ggtgcaacct gccggggata gccagaccaa ggcgatgaat
     1081 tgtcgcaggg gtggcagtta tacacctgcc gccccatttg tgaggcgttc agcagacaat
     1141 tcagagcctt ctgatgcctt aaaaaacctc ttgccgaagg caagtttaag agttaatcgt
     1201 gaaaggcatt tgtccaattc acgctgaacc aatacaggga catttaccga agtgattcag
     1261 cggtatatga actggaatga aaaagccaaa aggtgcagaa atactaaaga agatctgcgg
     1321 agtattggtt ctattaggtg gtatctggct tatttatacc gctccttaat ggaggatgcg
     1381 gttgggttaa gctgaataaa caggagagat tttatggata gaagaaactt tttagcacaa
     1441 agtatcgtag caggcggaac tgtggctctc ggatccgtaa ctcagacgga aaccaaatcc
     1501 gaagagaaaa tcaaaattat aggcgtcagt tgcagtccac gtaaaggtaa aacgacagcc
     1561 gctgcggtac aagcggctct ggatgcggca agggaaaccg acagccggat agaaacagaa
     1621 cttattgatc tcggtggcat gaaaattgcc ggctggtccg gcggggccaa accggactcc
     1681 cctcaaatcc ccttcgatga ctatgaagtg gaagtggaac cgagctttac ggctccgaat
     1741 ctgggcgggc tgattatcgg ttcgcctgtg tatttccgca gtatgagtgc cctgtgcaag
     1801 gcttttcttg aacgtcttta tactatgcgg actcctacga tgaggttggc agacaagccg
     1861 gttggcgcac tggctgttgg ctccttccgt aatggaggac aggaacttgt cattgaacag
     1921 attatcacag ccatgctttg ctatgaaccg cttattgtcg gcggtaaacc taatgctcat
     1981 caaggtgcaa cgctttggaa taattataat gactacatta ccaaggatga attcggcatt
     2041 aaaaccgcaa agcaattagg tattcgggta gctgaagctg ctttgaaaat caaaagagta
     2101 tagcccttat caagttggga ttcataattt gggatggtca aggaacatcg tatccccctc
//
LOCUS       DAOTBO010000239         2047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_41372_length_2047_cov_2.254016, whole genome shotgun sequence.
ACCESSION   DAOTBO010000239 DAOTBO010000000
VERSION     DAOTBO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41372_length_2047_cov_2.254016"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLE88_13505"
     CDS             <1..818
                     /locus_tag="PLE88_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOM77524.1"
                     /translation="AAERVFAVIDEPREPEVPGAFDLKPLQKDIVFEDIAFTYPGSLQ
                     PALKGVNLTVPAGKTVAVVGPNGSGKTTLVNLIPRFFEPDSGRILIDGQDIHKASLAS
                     LRDQISLVTQQVVTFNDTIANNIAYSKPGASMDEIVRAAKMAYAHEFIEPLPDSYNTL
                     IGESNAGFSGGQLQRIVIARAILKNPKILIFDEAMSQIDADSEAKIHAALEAIMKDRT
                     CFLIAHRFSTVISADFIVVMDGGRIAAQGRHEELMASCGLYKRLYETQLIGAV"
     gene            839..1648
                     /locus_tag="PLE88_13510"
     CDS             839..1648
                     /locus_tag="PLE88_13510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOM77525.1"
                     /translation="MEKKTVTICVVNYKTLDLTRLCLRSIRKYTDYPYKVLVIDNDSQ
                     DDSTEYLKSLKWIQFVSRTEKTNDSSGGYAHAAALDMGLALCDTPYFMAMHSDTFVHK
                     AGWLTELMRYFQTDPSVVCVGGGKCELTSSFREFLKKLTDFKTFRRKLFRTPDPLGVY
                     RYYNRTVCTIYRTDVLKKENLSFLMDREKGLTVGKKLYFELVDRGYKTVELPDSVMRR
                     YVWHLAHATQAVNPGEFALGGHTRNKALRLMDKIWSCPQVQEIIQDAALDA"
     gene            1685..1855
                     /locus_tag="PLE88_13515"
     CDS             1685..1855
                     /locus_tag="PLE88_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77526.1"
                     /translation="MANPVGTFIKKKIIGKDQNLISLDEPYEAMRRLLVNHSVTGILD
                     AGPAGDIFPNGC"
BASE COUNT          504 a          509 c          578 g          456 t
ORIGIN      
        1 cggctgccga gcgggttttt gcggtcatcg atgagccgcg cgagccggaa gtgcccggag
       61 cgtttgactt aaagccgctt cagaaggata ttgtgttcga agatattgcc tttacctatc
      121 ccggctccct gcagccggcc ctcaaagggg taaacctgac cgtgccggcc ggcaagacag
      181 tggctgtggt cggccccaac ggttcaggca agacaacgct ggtcaacctg attccccgct
      241 tttttgagcc ggacagcgga cggattctca ttgacggaca ggatattcac aaggcctcgc
      301 tggccagctt gcgggaccaa atcagtctgg ttacacagca ggtggttacc ttcaacgaca
      361 cgattgccaa caatatcgca tacagcaaac ccggcgcctc gatggatgag attgtgcggg
      421 cggccaagat ggcctatgcc catgaattta tcgagccgct gccggacagc tacaacaccc
      481 ttatcggcga aagcaacgcc ggtttttccg gcggccagct ccagcggata gttattgcgc
      541 gggcgatact gaaaaatccg aaaatcctga ttttcgatga ggcgatgagc cagatagacg
      601 ccgacagcga agccaaaatt cacgctgctt tggaagcgat tatgaaagac cgcacctgct
      661 ttttgattgc ccaccgcttc agcacggtca tttccgccga ttttattgtg gtgatggacg
      721 gcggacgaat agccgcacaa ggccggcatg aggaactgat ggcctcctgc ggtctctaca
      781 agcggcttta tgaaacgcaa ttgatcgggg ctgtttagcc gcatcggaga aagcagctgt
      841 ggaaaaaaag acggtaacaa tctgcgtggt caactataag acgctggatt tgacgcggct
      901 gtgcctgcgg agcatccgaa aatatacgga ctatccatat aaagtacttg ttattgataa
      961 cgactcgcag gatgactcga ccgaatattt aaagtcgctc aaatggatac aatttgtcag
     1021 ccggacagag aaaaccaacg attcttccgg cggctatgcc catgcagcgg cgctggatat
     1081 gggactggcg ctgtgtgata ccccgtattt tatggcgatg cattccgata cgtttgtcca
     1141 taaagccggc tggctgacgg agctgatgcg gtatttccag acggatccgt cggtggtgtg
     1201 tgtgggcggg ggcaagtgcg aattaaccag ctctttccgc gagtttctaa agaagctgac
     1261 ggatttcaag acattcaggc gaaaactttt ccgcacgccc gatccgctgg gtgtctatcg
     1321 ctattacaat cggacagtct gcaccattta ccgcacagat gttttgaaaa aggagaatct
     1381 gtcgttttta atggacagag aaaagggatt aaccgtgggc aagaagctgt attttgaact
     1441 ggtggatcgg ggctataaga cggtcgaact gcccgacagc gtgatgaggc ggtatgtctg
     1501 gcatctggcc catgcaacgc aggcggtcaa tccgggtgag tttgcacttg gcggccatac
     1561 ccgcaacaag gcgctgcgtc tgatggataa gatatggtcg tgcccgcagg tgcaggagat
     1621 tatacaagat gcggcattgg atgcataatg cacaaacaca cattagcagg agataaggag
     1681 tactgtggcc aatccagtgg ggacatttat caagaaaaag atcatcggca aagaccagaa
     1741 tctgatatcg ctggatgagc cgtatgaagc aatgcggcgt ctgctggtga accattcggt
     1801 tacagggata ttggatgccg ggccagccgg ggacatattt cccaacggct gctgaggaaa
     1861 tttccgtcgg cccaagtgta tgccttcgaa ccgcatccga tgtatcggcc ggtgcttgaa
     1921 gcatatgccg accgagaatc ttggtttcat cctttttttt gcagccctgt ctgaccatga
     1981 aggaacggcc cagctgcata taaccgaatc ggcgggcaac acatcaatcc tgaatcccgg
     2041 ccggcag
//
LOCUS       DAOTBO010000240         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_41682_length_2034_cov_4.465387, whole genome shotgun sequence.
ACCESSION   DAOTBO010000240 DAOTBO010000000
VERSION     DAOTBO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41682_length_2034_cov_4.465387"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..769)
                     /locus_tag="PLE88_13520"
     CDS             complement(80..769)
                     /locus_tag="PLE88_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOM77527.1"
                     /translation="MQMIRPIITLRKKRILIDVDTQRDLFVADGRACVRNHRRILANI
                     RRVMAWARKYKIRIISTAQIRSPDRHLGSYCVAGTDGVHKIRYTLRSRHVIFEPSDST
                     DMPRELIRDYDQIILCKRTDDPFEEPRAERVLTDARASEFIVIGGLVETSVLYTVLGL
                     LVRNKPVTVIIDAIGAREKTAADVALRKMQAKGAKLIDSKSLLGSSHLRLVNACTCDR
                     CRCRQQEKTVN"
     gene            960..1592
                     /gene="trmB"
                     /locus_tag="PLE88_13525"
     CDS             960..1592
                     /gene="trmB"
                     /locus_tag="PLE88_13525"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOM77528.1"
                     /translation="MGSKHLQNYPNIAVMPEELNGMIDFSVFFGHTGPVELEIGSGRG
                     TFLVSQAAAFAETHFLGIEWAAKFWRYAVDRIGRRGLDNVRLIRTDAAAFIRQHIPDA
                     SIRMVHLYFPDPWPKKRHHKRRFFCDENLCQLYRILEPDGIINTATDHADYFEQMTIV
                     AERAVAQGLFEQIPFIRPAGAQDGETVGTNYERKYRKEGRRTYTLALRKR"
     gene            1589..>2034
                     /gene="rsmG"
                     /locus_tag="PLE88_13530"
     CDS             1589..>2034
                     /gene="rsmG"
                     /locus_tag="PLE88_13530"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOM77529.1"
                     /translation="MNTAPLDLKPACQALEAYRAQFDKFAQMLIEANQTRNLTRITDP
                     SHIPVRHFLDSLAALAILDQLAGQTGRPLRIADIGSGAGLPALALAIARPDWAIVSVE
                     ATEKKVQFQQAVCAALKLTNVQLLTARAEIVAHSPDGRQRFDAATAR"
BASE COUNT          432 a          577 c          574 g          451 t
ORIGIN      
        1 ggaccagctg cggctttacg atgcgccgct cagcgcagag gacatagccg cactggcggc
       61 agattgaaca aggcagctgt cagttgacag ttttttcctg ctggcggcag cggcagcggt
      121 cgcaggtgca ggcattgacc agccgcaaat ggcttgagcc cagcagggat ttgctgtcga
      181 tcagcttggc gcctttggcc tgcatcttcc gcagggcgac atcggcagca gttttctccc
      241 gtgctccgat ggcatcaata atgacggtaa caggcttgtt gcgaaccagc aggcccagca
      301 cagtataaag aaccgatgtt tccaccagcc ctccaatcac gatgaattcc gacgcccgcg
      361 catcggtcag cacccgctcg gcacggggtt cttcaaaggg atcgtcagtc cgtttgcaca
      421 gaatgatttg gtcgtagtcg cgtatcagtt cacggggcat atcggtgctg tcgctgggct
      481 caaaaatcac atgccggctg cgaagcgtat agcggatttt atgaacgccg tcggtcccgg
      541 caacgcagta actgcccaaa tgtctgtccg gactgcgtat ctgcgcggtc gagatgatgc
      601 ggattttgta tttgcgggcc caggccatca cccggcggat gttggctaaa atccgccggt
      661 ggttgcggac gcatgctctg ccgtcggcca caaataaatc gcgctgcgta tcgacatcga
      721 ttaaaatcct ttttttgcgc aatgtaatga tcggtctgat catctgcatt ccgaaccctt
      781 tataattaag gcctgttccc gagttctata ccctcctata cgtcttggcc ttttgagcgg
      841 ttctcattta tagctatcga acagatgagg gcttgacttt atcggttttt gggaacaaaa
      901 taagacaaga ccttggctcg taaagccgtt tttgcaggat tcttacaaaa tcttttctta
      961 tggggtccaa acacttacaa aactatccga atattgccgt tatgcccgaa gagctgaacg
     1021 ggatgattga tttttcggtt tttttcggac atacgggacc ggtcgagctg gaaatcggct
     1081 ccggcagggg gacctttctg gtcagtcagg cggccgcctt tgccgaaacg cattttctgg
     1141 gcatagaatg ggcggccaaa ttctggcgct atgccgtcga ccgcatcggc cggcggggac
     1201 tggacaatgt gcgcctgatt cgcaccgatg cggcggcctt tatccgtcag catatccccg
     1261 atgcctcgat acggatggtg catctgtact ttcccgaccc gtggcccaaa aagcggcatc
     1321 acaagcggcg ttttttctgc gatgaaaacc tctgccagct ttaccgcatc ctcgagccgg
     1381 atggaattat caacaccgcc accgaccacg ccgactattt tgagcagatg acaattgtcg
     1441 ccgagcgggc cgttgcacag gggctgtttg agcagatccc ctttatccgg ccggccggcg
     1501 cacaggacgg cgaaaccgtc ggcaccaatt atgaacgcaa atataggaaa gaggggcgca
     1561 ggacctacac gcttgcgctg cggaagcgat gaacacagcg ccccttgacc tcaaaccggc
     1621 ctgtcaggct ctcgaagcat accgggcgca gttcgataaa ttcgcccaaa tgcttatcga
     1681 agccaaccaa acccgcaatc tgacgcgcat tacggacccg tcgcacattc cggtgcggca
     1741 ttttctcgat tcgctggcag cactggcaat tcttgaccag ctggccggac aaaccggcag
     1801 gcccctgcgg attgcagaca tcggctccgg tgcaggtctg ccggcgctgg cgctggcgat
     1861 tgctcggccg gactgggcga ttgtctccgt ggaagccacc gaaaagaagg tgcagtttca
     1921 gcaggccgtc tgcgcggcgc tgaagctgac caatgtgcag ctgctgaccg cgcgggcaga
     1981 gattgtggca cacagccccg acggtcggca gcggttcgat gcggcaacgg cacg
//
LOCUS       DAOTBO010000241         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_41984_length_2023_cov_4.956809, whole genome shotgun sequence.
ACCESSION   DAOTBO010000241 DAOTBO010000000
VERSION     DAOTBO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41984_length_2023_cov_4.956809"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(553..1524)
                     /locus_tag="PLE88_13535"
     CDS             complement(553..1524)
                     /locus_tag="PLE88_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77530.1"
                     /translation="MAILVGIDEAGYGPLLGPLVVSSVVLEMPEECLRADLWKLLGQA
                     VCAHKKGLAGRLLVNDSKKVYTRSSGIGPLRRTVLSCLNALDPPACPRTAAELIGFLC
                     PDAAERLQADPWYARLDQQPLGQNQTDTAIAGSMLRKTMSENNMRICAVRSRCMDAGF
                     YNQRVEQVKNKSRVLFTEVCSLVLAAFSSEAQAGQPMQVIIDRQGGRINYQQDLLRMF
                     PGYSLSVLRQDDKMSSYELSQSSKIMRIHFCLKADSKYMCVSLASMVSKYLREVLMDA
                     FNAHFCGLCSSLKPTAGYWEDGQRFLKDLSDLLPDYQYDPQKMVRIL"
     gene            complement(1547..2005)
                     /locus_tag="PLE88_13540"
     CDS             complement(1547..2005)
                     /locus_tag="PLE88_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOM77531.1"
                     /translation="MLLTQILQPVCIKAPLAGKTKEDAIEELVNLLADHGYLTDRADA
                     FEAVMAREHTRSTGIGSGIAIPHGKCSAAKELVMAVGIAAEPIDFNSIDGKPVTIILL
                     LVSPPDQTGPRIQALARISRLMLDAQFKAQLEKAASPEEVYSLISSKESE"
BASE COUNT          527 a          484 c          503 g          509 t
ORIGIN      
        1 ctatgctaac ggaagggacg ctttatttgt cgtggagaaa ttatagttga cgctgaacag
       61 tccgccgtgg ggcgttaagt cgggcataag aaagggtatt ttcctgcctt gactgccggc
      121 aggagacagc taatcgggaa aaatcgcttt ttgggaaaac gttcggattg ataatctggc
      181 agcgtttagt taacgctgga aaaaaaaaga ccgcccgtac ttcgcagaag gaggaggaag
      241 tcggtgaact tcccagggct acagaagtta cgggcggcag aaggagcttt ctatcctttg
      301 tatttgtccg gccggtaaca aaacctctgg acaaagaagt tatttcacag tcatattagc
      361 attaatataa agtatatgca acaaaaaaag aaaagtttgg ataaaaattt ccgtagaact
      421 acggattttg tgcgagttat aatccctact ttttatccat tttaaaaaaa tacctgattt
      481 agaatagaca ggatttagtc gtcagccggc tggaagttta cataaaactt gtatctgcac
      541 tcgatatatc ttctataaga tgcgtaccat tttttggggg tcgtattgat aatcgggcag
      601 taaatcggat aaatctttga ggaaacgctg gccatcttcc caatagccgg cggtaggttt
      661 taaactgctg caaagcccgc agaaatgggc attaaatgca tccatcaaaa cttcccgcag
      721 atacttgctg accatcgatg ccagcgatac gcacatatat ttggaatcgg ctttcaggca
      781 gaaatgaatc cgcataattt tgctggactg gcttagttcg taactgctca ttttatcatc
      841 ctgccgcaga acagacaggg aataaccggg gaacatccgc agcaaatcct gctggtagtt
      901 gatgcgtccg ccctgacggt caatgataac ctgcatcggc tggccggctt gggcctcgct
      961 gctgaaggcc gccagcacga gagagcaaac ttcggtaaag aggaccctgc ttttgttttt
     1021 aacctgctct acacgttgat tgtaaaagcc cgcatccata cagcggctgc ggacggcaca
     1081 aatccgcata ttgttttcgc tcatcgtttt tcgaagcatg ctgccggcaa tagcggtatc
     1141 cgtctgattc tgtccaagcg gctgctggtc cagccgggca taccaaggat cggcctgcag
     1201 ccgttccgca gcgtcaggac acaggaagcc aatcaattca gcggctgtac gcgggcatgc
     1261 cggcgggtcg agagcattca gacaggacaa gactgttcgg cgcagcggcc cgatgccgct
     1321 gcttcgggta taaacctttt tgctgtcatt aacaagcagg cggccggcca atcctttttt
     1381 gtgcgcacaa actgcttggc ccagcagttt ccataaatca gcacgcagac attcctccgg
     1441 catctccaaa acgactgatg aaactaccag cggacccaaa agcggtccat aacctgcttc
     1501 atcaatgcct accaggatag ccataccaaa aacctgacag gaagaactat tcggattctt
     1561 ttgagctgat tagagaatat acctcttcgg gagaagcggc cttttccagc tgtgccttaa
     1621 actgggcatc gagcatcagg cggctgatgc gagccaatgc ttgaatgcgc ggtccggttt
     1681 gatccggcgg tgaaaccagc agcagaatta tggtgacggg tttgccgtca atactgttga
     1741 aatcgatggg ttcagcggca atcccgacgg ccataaccag ctctttggcc gcgctgcatt
     1801 tgccgtgcgg aatggcgatt cctgagccga ttcctgtact tcgggtatgt tcgcgggcca
     1861 ttactgcctc aaaagcatcg gccctgtcag tcaaataacc atgatcggcc agaagattta
     1921 ctaattcttc aatggcatct tcttttgtct tgccggcaag aggtgcctta atgcagacag
     1981 gctgcagaat ttgtgtcaaa agcatttttt attgattacc tgt
//
LOCUS       DAOTBO010000242         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_42258_length_2013_cov_9.273238, whole genome shotgun sequence.
ACCESSION   DAOTBO010000242 DAOTBO010000000
VERSION     DAOTBO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42258_length_2013_cov_9.273238"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..262)
                     /locus_tag="PLE88_13545"
     CDS             complement(134..262)
                     /locus_tag="PLE88_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77532.1"
                     /translation="MRRWATVPTAIGRHARSRKFGMAVAADGDSICPMPPMGADSL"
     gene            482..1483
                     /locus_tag="PLE88_13550"
     CDS             482..1483
                     /locus_tag="PLE88_13550"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOM77533.1"
                     /translation="MGRHIAIAGVTGAVGQEFLKIIEERKFPFDSIKMLASSRSAGKK
                     IEFMKKTYIVEELTANSFKGIDIALFSAGGARSKEFAPAAVKAGAVVIDNSSAFRMDP
                     QVPLVVPEINPEAIRQHKGIIANPNCSTIIANVPVWPLHKANPVKRMVVSTYQAASGA
                     GQSAMLEMLQQAREVLDGKPVTCKALKYQLAFNLYCHDSKIGPNGYNEEEMKMVKETQ
                     KIFNCPSIAITCTCVRVPVLRTHCESINLEFTHPITPDQVRDLLSTAPGVSIIDDRAN
                     NRFPMPLDATGKDDVYVGHIRQDESIPDNRGINLWVAGDQIRKGAALNAIQIAEKLL"
     gene            1549..>2013
                     /locus_tag="PLE88_13555"
     CDS             1549..>2013
                     /locus_tag="PLE88_13555"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOM77534.1"
                     /translation="MAFRRIKLEIHYDGTVYHGWQRQPQQLTIQETLEAALERLCGQK
                     IEVIGSSRTDAGVHALGQVAHFDFDSPIPTNRIAKALNRLLPDNIAIAEAVEVGSDFD
                     AVASTKRKWYRYTIYTAPIRPVMEIRYCWHRPGHLDAAAMQAAAARLIGTFDF"
BASE COUNT          529 a          510 c          495 g          479 t
ORIGIN      
        1 tgcatctgag acctgtgaat acctgcgcct atggattttt agctcattat ccataggcac
       61 agactgtgag tatggatttt tagccaaatt ttccatagat acaggtattt acatatattc
      121 acatataatt ccctcatagg gagtctgccc ccatcggcgg catcggacag atagagtcgc
      181 catctgccgc cacagccatc ccgaacttgc gacttcttgc atgccgtccg atagccgttg
      241 gaactgtcgc ccaacgccgc attttgccgc aaacctttta caggaaatgc tctatatcac
      301 acccaatagt gccagtactt gtttgcactt attgcatccc taatcggttt taaccttcaa
      361 ggtaaaaaga cccgactctg gttccatcaa aaaaatgttg aaatattcat caatggttgg
      421 taacatataa gtttttaccg tgatagtata aataaaactt atttattcag gagtaaatat
      481 aatgggcaga catattgcca tagccggcgt aaccggcgct gtcggacaag agtttctgaa
      541 aattattgag gagcgcaagt tcccgtttga ctccataaag atgctggcca gttcgcgttc
      601 ggccggcaaa aaaatagagt ttatgaagaa aacctacatc gtcgaggaat tgacagccaa
      661 cagttttaag gggattgata tcgccctgtt ttccgccggc ggggcccgca gcaaggaatt
      721 cgctcctgct gccgtaaaag ccggggctgt tgtgattgat aattcatcgg cttttcggat
      781 ggatccgcag gtgccgctgg ttgttcccga aataaatccg gaagctatca ggcagcacaa
      841 aggcatcatc gccaatccga actgctcaac catcattgcc aatgtacccg tttggccgct
      901 gcataaggcc aatcctgtca aacggatggt agtcagcacc tatcaggcag ccagcggagc
      961 tgggcagtcg gccatgctgg aaatgcttca gcaggcccgt gaagttctgg acggcaaacc
     1021 cgttacctgc aaggccctga aatatcagct ggcgttcaat ctgtactgcc atgactctaa
     1081 aattggaccg aacgggtata atgaagaaga aatgaagatg gttaaagaaa cccagaaaat
     1141 cttcaactgc cccagcattg ctattacctg tacctgtgta cgggtgcctg ttttgcggac
     1201 gcactgcgag agcattaatt tagagtttac gcatccgatt acccctgacc aggttcggga
     1261 cctgttgagc acagcgccgg gcgtgagcat tatagatgac cgggcaaata accgttttcc
     1321 aatgcctctg gatgctaccg gcaaagatga tgtatatgtc ggccacatcc ggcaggatga
     1381 atcgataccc gacaatcggg gcatcaatct ctgggtggca ggcgaccaga ttcgcaaagg
     1441 agccgctctg aatgccatcc aaattgcgga aaaactgctg taaagtaaga acatagaacg
     1501 cagaaaacag acgccagcac gcctgttttt ctgtgttctg ggttctttat ggctttcaga
     1561 cgaataaaac ttgaaattca ttatgacggg acagtctatc acggctggca gcggcagccg
     1621 cagcagctga caattcaaga gacgctggaa gctgcattag agcgtctttg cggtcagaaa
     1681 atcgaggtca tcggctcatc gcgaacagat gcgggcgtgc atgccctcgg gcaagtagcc
     1741 catttcgatt tcgacagtcc catcccaaca aaccgcatag ccaaggcact taacaggctg
     1801 ctgcctgata atatcgctat tgccgaagcc gttgaagtcg gctccgactt tgatgctgtt
     1861 gccagcacca aacgcaagtg gtaccgctat acgatctaca ccgccccgat tcggccggtg
     1921 atggaaatcc ggtactgctg gcaccggccg ggacatctcg atgcggcagc aatgcaggcg
     1981 gccgcagcgc ggctgattgg tacgttcgac ttc
//
LOCUS       DAOTBO010000243         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_43864_length_1955_cov_3.387895, whole genome shotgun sequence.
ACCESSION   DAOTBO010000243 DAOTBO010000000
VERSION     DAOTBO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43864_length_1955_cov_3.387895"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..872
                     /locus_tag="PLE88_13560"
     CDS             207..872
                     /locus_tag="PLE88_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOM77535.1"
                     /translation="MAEKLNKNPVCSGKDDARERLLDAAEKLFCLKGFNRTSVRELTA
                     EARCNLAAVNYHFKSKEHLYVEMFRRQFEAMIRSRLEAIDYVMQLPQADLEGLLRILI
                     EPPIRRIDEDPEHTQVMRLLVREVLNRQIDPKLIALELKEKLFDRLGEALKRLVPNLP
                     AEKLMLIVWSVDGVLLHSFLFMEFYREAMPKLTLNELIEHLVRFSASAIRGYSKGYVS
                     CSE"
     gene            860..>1955
                     /locus_tag="PLE88_13565"
     CDS             860..>1955
                     /locus_tag="PLE88_13565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOM77536.1"
                     /translation="MQRMKDCFLKGLAVLCSLAGCTVGPDYQPPAPVLPDMWAEAALM
                     QQGEPNLLKEWWVVLDDPVLMRLIEQASQGSRNLRQAYERIAESRALRDYAAGRYYPA
                     ADFSASYTRSRESANTAFAFPAMLQEHGSYTGGFDAAWELDLFGGIRRSVESAQALLE
                     ASVENFRDVQASLYAEVAADYVELRTVQMRLQFAMDNIKVQKDTLTLTEDRYKAGLAP
                     ELDVAQARQNLANTEAQVPSLRTAEIQVLNRLAVLLGQYPQELDEVLKMPAAIPLPAS
                     DKLPSALPADLLRQRPDIRRAERQLAAQTAQTGVTVSQLYPSFSLWGSFALVAERFPD
                     VGNWSSRSYSYGPSLGWRVFEGGRIRNMVKA"
BASE COUNT          466 a          445 c          543 g          501 t
ORIGIN      
        1 ccgactctgc taattgcagc aatttttcca tagttgggaa cagcgtatca tctgaataat
       61 ttacaacaat aaaggcctgc aataattaga attatttacg ttatcaaata aaattgcagc
      121 atttaaaagt ttttttggga aaatatttga caatcgaaca tatgttatga aacaattgtt
      181 tgaaacgatt attttaatac aaagaaatgg ctgaaaaact aaataaaaat cctgtatgtt
      241 ccggcaagga tgacgcccgc gaacggctgt tagatgcggc agaaaagctc ttttgcctta
      301 aaggcttcaa tcggacttcg gtgagggaac tgactgccga ggcacgctgc aatcttgcgg
      361 ccgtcaacta ccacttcaaa agcaaggagc atttatatgt ggagatgttc cgcagacagt
      421 ttgaagcgat gatccgcagc cgattggaag cgattgatta tgtaatgcag ttgcctcaag
      481 cggatttgga ggggcttttg aggattctga ttgaacctcc aatacggcga atcgatgaag
      541 atccggaaca tacacaggtc atgcgtctgc tggttagaga ggtcctgaac aggcagattg
      601 accccaaatt aattgctctc gagttaaagg aaaagctgtt cgatcggctg ggagaggcat
      661 tgaagcggct ggttcccaat ctgccggctg agaagctgat gttgattgtc tggagtgtag
      721 atggcgtgct gctgcattcg tttttgttta tggaatttta ccgggaagct atgcccaaat
      781 tgaccctgaa tgaattgatt gagcatctgg tacggttttc cgcttcggcg atacgcggtt
      841 attcgaaagg atatgtgtca tgcagcgaat gaaagattgc tttttgaaag gacttgctgt
      901 gctgtgcagt ctggcaggat gtacggttgg gcctgattat cagcccccgg ctccggtttt
      961 gccggacatg tgggctgagg cggcactgat gcagcagggg gagcccaacc ttttgaaaga
     1021 atggtgggta gtactggatg atcctgtgct gatgcgtttg attgagcagg cctcccaagg
     1081 cagccggaat ctgcggcagg cttatgagcg catcgctgaa tcacgggcct tgcgggatta
     1141 tgcagcagga agatattatc ctgctgccga tttcagcgcc tcatataccc gaagccgtga
     1201 aagtgccaac accgcattcg cttttccggc aatgcttcaa gagcatggct cctataccgg
     1261 cggttttgat gctgcatggg aactggattt gttcggaggc atccggcggt cggtagaatc
     1321 ggcacaggcc ctgctggagg catctgtcga aaacttccgt gatgtgcagg caagtctgta
     1381 tgctgaagta gcggcagatt atgtcgaact gcggactgtt caaatgcggc ttcagtttgc
     1441 catggacaat atcaaagtgc agaaagatac cctcacgctt accgaagacc gctataaagc
     1501 cggtctggct cctgaactgg atgtggcaca ggcccgccag aatcttgcca ataccgaggc
     1561 ccaagtgcct tccctgcgaa cggctgaaat acaggttttg aaccgtctgg ctgtgctgct
     1621 cggacagtac ccgcaagagc ttgacgaggt attgaaaatg cctgcggcga ttccgcttcc
     1681 ggcctccgat aagcttccat cggctcttcc ggcggatctg ctgcgtcagc ggcctgatat
     1741 tcgccgggct gagcggcaat tggcagccca gaccgctcaa accggcgtca cagtttccca
     1801 gctgtatccc tccttttcgc tgtgggggtc ttttgcgctt gtcgcagagc ggtttcctga
     1861 tgtcggcaat tggtccagcc gcagttacag ttacggacct tcgctgggat ggcgggtttt
     1921 tgagggcggg cgcattcgca atatggtcaa ggccg
//
LOCUS       DAOTBO010000244         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_45735_length_1893_cov_4.794342, whole genome shotgun sequence.
ACCESSION   DAOTBO010000244 DAOTBO010000000
VERSION     DAOTBO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45735_length_1893_cov_4.794342"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLE88_13570"
     CDS             complement(<1..1467)
                     /locus_tag="PLE88_13570"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOM77537.1"
                     /translation="MKQQWMHRTGWLILSALLAVSWRGLGAERYWDIEYGTAGPVRLV
                     LDAGVPDGPLLRPAVILIHGGGWSSGDKKDMAFLTEPLSEAGLVWFSINYRLAPDYRW
                     PACLEDVKTAIRWVKSHAKEYKADASRIGLIGYSAGGHLACMAAVTADEQTRVQAVVG
                     IAPPTDHEADSRRRGGLSPSLQKLLDRQQEITDETRLLLQRISPVQYVNAGLPPFLLI
                     HGTEDTSVPYSQSIEFKAKLDHYNAPCRLIALQGAGHRLSDWPTQDYLAEMINWLKQA
                     LDSERRVPLQAPAPRTILAKTVAQDGSGDFTSVQAAVDAVPDGNEDSIVIAVKPGVYK
                     ERIVVPRHKRFICFKGTEASDTILTFDLYAGIQDAQGKEIGTFRTPSVTIEADDFTAE
                     NITFENSAGPVGQAVAIAVFGDRAVFRNCRFLGWQDTILDHLGRHYYENCYIAGHCDF
                     IFGGGTAFFEKCRIECLKNGYITAASTPEKQPFGYVFSN"
     gene            complement(1525..>1893)
                     /locus_tag="PLE88_13575"
     CDS             complement(1525..>1893)
                     /locus_tag="PLE88_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77538.1"
                     /translation="ELIEDTDDQRIGMLEDVAQSYLNGLPYKVPLEACRPFTLCVNGA
                     FDSSGQTVFIDASYVVKASRNGLPTTLIRDIVPIMQRAHAESQLLSEIRIPWAQKSRT
                     VGLKEYAYFPSGQAASHAAG"
BASE COUNT          428 a          582 c          461 g          422 t
ORIGIN      
        1 attgctgaag acatatccaa acggctgctt ctcgggtgtc gatgctgctg ttatataccc
       61 gtttttgaga cattcaatcc tgcatttttc aaaaaatgct gttcccccgc cgaagataaa
      121 atcgcagtgc ccagcaatat agcaattctc ataataatgc cgtcctaaat gatccaaaat
      181 ggtatcctgc cagcccaaaa atcggcaatt gcggaaaaca gcacggtcgc caaacacagc
      241 aatagccacg gcctgcccta ccggccctgc cgagttttca aaggtaatat tttctgccgt
      301 aaaatcatcc gcctcaatcg ttaccgaggg tgtgcggaaa gtgccgattt ctttgccttg
      361 tgcatcttga atgcctgcat acaaatcaaa tgtcaaaatg gtatctgacg cctcagtacc
      421 cttaaaacag ataaaccgct tatggcgggg cacaacaatc cgctccttat aaacaccggg
      481 cttgacggca atcacaatgc tgtcttcgtt gccgtcaggc acggcatcca cagcggcctg
      541 cacagaggta aaatccccgc ttccgtcttg ggcaacggtt ttagcaagga tcgttcttgg
      601 tgcaggcgcc tgcaggggca cgcgacgctc actatccagc gcctgcttga gccaattgat
      661 catctctgcc agataatcct gtgtcggcca atcggacaag cgatggccgg ccccctgaag
      721 cgcaatcagc cggcaggggg cattgtagtg gtccagtttg gccttgaact ctatcgactg
      781 ggaatacggc acgctggtat cctcggtgcc gtgaatcagc agaaacggcg gcaggccggc
      841 attgacatac tgaacaggtg aaatccgctg caacagcagc cgcgtttcat ccgttatctc
      901 ctgctgccgg tccagcagct tttgcagcga tggactcagg cccccccgcc gccggctgtc
      961 tgcttcgtgg tcggtcggcg gcgcaatacc cacaacggcc tgtacccgtg tctgttcgtc
     1021 tgccgtaaca gcggccatac aggccagatg cccgcctgcc gagtatccaa tcagcccgat
     1081 gcgggaagca tccgctttgt attccttcgc gtggctctta acccaccgaa tcgctgtttt
     1141 aacatcctcc agacaggcag gccaccgata atccggtgcc agccggtaat tgatcgaaaa
     1201 ccacaccaag cccgcctccg acagcggctc ggttaagaac gccatatcct ttttgtcgcc
     1261 gctgctccag ccacccccgt gtatgagaat caccgccgga cgaagcagag gcccatccgg
     1321 cacaccggca tccagcacca gccgcaccgg ccctgctgtg ccgtattcta tatcccaata
     1381 ccgttctgca cccaagcccc gccaactaac cgccagcaga gcagataaaa taagccatcc
     1441 tgtcctgtgc atccattgct gtttcatcac tatacaatcc ttgtccgttt cgtctgtctt
     1501 cgtatgttgg gagtcaatct gctgtcagcc ggccgcatgg gaagccgcct gcccgctggg
     1561 aaaataggca tattccttca gaccgaccgt ccggcttttc tgcgcccatg gtatccggat
     1621 ttccgacagc aactgggatt cggcatgcgc tctctgcata atcggcacaa tatcccgaat
     1681 caacgtggtc ggcagtccgt tgcggctggc tttgaccaca taggacgcat cgataaagac
     1741 ggtctgcccg ctggaatcaa aggcgccgtt gacacagagc gtaaacggcc ggcaggcttc
     1801 cagggggact ttataaggca gcccgttcag ataggattgt gccacatctt ccagcattcc
     1861 gatgcgctgg tcatcggtat cctcaatcag ttc
//
LOCUS       DAOTBO010000245         1890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_45822_length_1890_cov_4.380926, whole genome shotgun sequence.
ACCESSION   DAOTBO010000245 DAOTBO010000000
VERSION     DAOTBO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45822_length_1890_cov_4.380926"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1276)
                     /locus_tag="PLE88_13580"
     CDS             complement(134..1276)
                     /locus_tag="PLE88_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOM77539.1"
                     /translation="MTNTGRFFGCLIAAGLLFYSGCGSKPKGKFTEEQMRQIPLANKT
                     NLPQATGGMVLGVYSETISSDEILLAVEDYIKPDAAVLDQQRFEAKALPIVRRAIRGK
                     ITDILIYREARQKAPDNIDENLDKAVEAETARFVASYGNNYALAESKLNEMGMDWRSF
                     RDYQKKMIMIQSYLSSTLKEEKRFSYQQLKDYYEQIKNAQYCREGRVGFSIIEIQPER
                     LTSQQCGGQTPDQAAQRIGADLVGQLNAGADFAQLARQYHGDLASVGGRIMPVAIGSD
                     SLTEPYQSLIQQALQMQPGQIAGPIPLKGRFFILKLDTLETSSCRPFEEVQNEVAQQL
                     QFERRKQEYETYVNRLIEKANIAQLDRFAAFCVQEAYRRWGLNTSS"
     gene            complement(1412..>1890)
                     /locus_tag="PLE88_13585"
     CDS             complement(1412..>1890)
                     /locus_tag="PLE88_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOM77540.1"
                     /translation="IAYVVDGCPQAAGICNPAAGQTFLGVRGQGITLNGKPAAVSQKQ
                     DLNGAAVSASRSEVNRGQWKPFENAPFRIIPMGSVAYKLARVAAGLEDMTFTLVAKNE
                     WDVAAGWLLVEAAGGIILDKNRRIRRFNQPNPLLDGLFAGNAAILQHLFNTTLQQQ"
BASE COUNT          391 a          519 c          509 g          471 t
ORIGIN      
        1 gcggcgtcgg cctgttcgcg cggggtaaaa atgcgttcga ggaaacgctc cggatgccgc
       61 tggagcatct gctcaatgcg ttcaaaatcc actaaatcga ttccgtgagc aatcattttt
      121 tccattgctg tgcttatgag gatgtgttta gaccccagcg gcggtatgct tcctgcacgc
      181 agaaagcggc aaaccgatcc agctgtgcga tgttggcctt ctcgataagt ctattgacat
      241 aggtttcgta ctcctgtttg cgccgctcga actggagctg ctgggcgact tcgttctgga
      301 cctcttcaaa cggccggcag ctgctggttt ccagcgtatc gagcttgaga ataaagaagc
      361 gtcctttcag gggtatcggg cctgcaattt ggccgggctg catctgcagg gcctgctgga
      421 tcagcgattg atagggttcg gtcagcgagt ccgagccgat agcgaccggc atgattcggc
      481 cgcccacaga cgccaagtcg ccgtggtatt gtcttgccaa ttgggcaaaa tcggcgccgg
      541 cattcagctg acccaccaaa tccgccccga tgcgctgggc ggcctgatcc ggcgtctggc
      601 cgccgcactg ctggctggtc agccgctccg gctgtatctc gatgatgctg aagcccaccc
      661 gcccttcccg gcaatactgg gcgtttttaa tctgttcgta atagtccttc agctgctggt
      721 aggaaaaacg cttttcctcc tttaaggtgc tggacagata cgactgaatc ataatcatct
      781 tcttctggta atcccgaaag gagcgccagt ccatccccat ctcgttgagc ttgctttcgg
      841 ccaaggcata gttgttgccg tagctggcta caaaacgggc ggtttcggct tcaacggcct
      901 tatccagatt ttcgtcgatg ttgtcggggg ctttttggcg cgcctcccga taaatcagaa
      961 tatctgtaat tttgccccgg atggctctgc ggacaatggg aagcgctttt gcttcaaatc
     1021 tctgctggtc cagcacagcg gcgtccggct tgatgtaatc ttccacggca agcagaatct
     1081 catcggagga aatggtctcc gaataaacac ccagcaccat cccgccggtg gcctgcggca
     1141 gattggtttt attggccaga ggaatctggc gcatttgttc ttcggtaaac ttgcctttgg
     1201 gttttgaacc gcagccggaa taaaaaagca atcctgcggc tataagacag ccgaaaaatc
     1261 tgcctgtgtt agtcatttat atcggctcca gaaagtccat attctttgtt cggaacgaaa
     1321 tgattatacg aaaaatccgg ccatttcaaa cttctttctc aaaaaggcgg tttgttttgg
     1381 acgcagggga aatgttgtgc cggattggat gctattgctg ctgcagggtg gtgttgaaca
     1441 gatgctgcaa aatcgccgcg ttgcctgcga ataaaccgtc caaaagagga ttgggctgat
     1501 tgaatcggcg aatccgtctg tttttatcca gtattatccc tcctgccgct tccaccagca
     1561 gccatccggc ggcaacatcc cattcatttt tggctaccag cgtaaaggtc atatcctcca
     1621 gtccggcggc aacacgagcc agcttatagg ccaccgaccc catcgggata atccgaaagg
     1681 gggcattttc aaacggtttc cactggccgc ggttgacctc gctgcggctg gcggataccg
     1741 ctgctccgtt caagtcctgc ttttgactga ccgcagccgg cttgccgttg agggtaatgc
     1801 cctgtccgcg aactcccaga aaggtctgtc cggccgcagg attgcagatt ccggccgcct
     1861 gcggacagcc gtccacgaca taggcaatcg
//
LOCUS       DAOTBO010000246         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_46092_length_1881_cov_4.625958, whole genome shotgun sequence.
ACCESSION   DAOTBO010000246 DAOTBO010000000
VERSION     DAOTBO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46092_length_1881_cov_4.625958"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PLE88_13590"
     CDS             complement(<1..1433)
                     /locus_tag="PLE88_13590"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOM77541.1"
                     /translation="MRNITVTGLGILLVLLFGPAVQAELQFVDNFEGMTVGANMPYNV
                     PTAGGGGIWSTNTGGTSGNIYVETNSGSTVIRFMTTSDGANSRGFGFADIDNAIENTE
                     SGILFFRFCIRVEANAADTYFGIHALSGVTPFAVAGNSPQNYIVAGFRAVNGAGTSVD
                     LVAIKDPGAVLLAGLTRGQWYNCWIDADNAANTFDLYISPASGPAGPPTLPTQADRIA
                     DDLPFENTATYGNSPLMGAFFDTPRLSSTTRSTTQSARTFIDEVWWDGDAGLVFISKG
                     ARNPVPANNATEVPLDQVLSWDAPDDPNVQAVLGYDVYFSSNQADVQNGEPSALVSAG
                     QTALSYKPVLAYDAHYFWRVTTTVQMDDPNHTVLEDPGRVWSFETMHSLPIITQQPAD
                     VIAPAGQTVQFAIAAESILPASYQWYKSSDRTSGTPDDDTLISGASSAVLTLSTISIS
                     DEAYYYCKVYNPSVVYSNAASLGVKRE"
     gene            complement(1497..>1881)
                     /locus_tag="PLE88_13595"
     CDS             complement(1497..>1881)
                     /locus_tag="PLE88_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77542.1"
                     /translation="GEWEQVTVTYDGSTARVYRNGQSAVSAAMPLSGGTDATVMIGAV
                     GLDLTTGLPISPMNGLLDDVWIYNYSLDPVTVAYSYTDIQGGTVCVYPNDAVLQAFDF
                     NHNCRIDVGDLVEMAAYWLYDQLVP"
BASE COUNT          481 a          506 c          454 g          440 t
ORIGIN      
        1 aattcccgtt tgacacctaa agaggccgca ttggaataga caacagaggg attataaact
       61 ttgcaataat aatacgcttc atcagatatc gaaatagttg acagcgttaa aacggctgaa
      121 gatgcgccgg aaatcagcgt atcatcgtcc ggtgtgccgc tggttcggtc ggatgattta
      181 taccactgat acgaagccgg caaaatactt tcagccgcaa tcgcaaactg gacagtctga
      241 ccggcagggg caattacatc ggccggctgc tgcgtaataa tcggcagcga gtgcattgtt
      301 tcaaaactcc acaccctgcc cggatcttcc aatactgtat gattcggatc atccatctga
      361 actgttgttg tgacgcgcca aaaatagtgt gcgtcatatg ccagaaccgg tttgtacgac
      421 agggctgtct gaccggcact aaccagcgct gaaggttcgc cgttctgaac atctgcctga
      481 ttggatgaga aatatacatc gtagcccagc accgcttgaa cattgggatc gtccggggca
      541 tcccacgaaa gaacctgatc taacggaacc tcggtggcgt tattggccgg caccggattc
      601 cgagcaccct tgcttataaa caccagtccg gcatcgccat cccaccagac ttcatcaata
      661 aacgtccggg cagattgagt agttgaccgg gtggtggaag acagacgggg tgtatcaaaa
      721 aatgccccca tcagcgggct gtttccatac gtcgcggtat tttcaaacgg cagatcgtct
      781 gcaattctgt cagcctgagt gggcagcgtt ggtgggcctg caggccccga agcagggctg
      841 atgtataaat caaaggtgtt ggctgcgttg tcggcgtcta tccagcagtt gtaccattgc
      901 ccgcgggtca gaccggctaa aagaaccgca ccgggatctt tgatagcgac caaatccacg
      961 gatgtgcctg caccattaac cgcacggaaa ccggctacaa tatagttttg aggactgttg
     1021 cctgcaacgg caaacggcgt gactccgctt agagcatgaa taccgaaata ggtgtcagcc
     1081 gcattagctt ccacgcgaat gcagaatcgg aaaaacaaga tgccgctttc tgtattttca
     1141 atggcattat caatatcggc aaatccaaaa ccgcggctgt tagcaccgtc ggaggttgtc
     1201 ataaagcgaa tgacagtgct tccgctgttg gtttcgacat aaatatttcc tgacgttcct
     1261 ccggtattcg tgctccagat gcccccgccg ccggcagtcg gaacattata tggcatattt
     1321 gctccgaccg tcatcccttc aaaattatct acgaactgca gctctgcctg aactgccggg
     1381 ccaaacagca gcacaagtaa aatgcctaat cctgttacag ttatgtttcg cattttacac
     1441 cctttctatc atccaaaaac cctctattct gtttatccga taggcagtct gcagtgttat
     1501 ggcacaagct ggtcataaag ccagtaagcg gccatctcga ccagatcgcc cacatcaatt
     1561 ctgcagttat ggttaaaatc gaatgcctgc aagaccgcat cgttcggata cacgcagact
     1621 gttccgcctt gaatatctgt ataggaatag gccaccgtga ccggatccag ggaatagttg
     1681 taaatccata cgtcatccag caggccattc attggcgaaa ttggcaagcc ggtggtcaaa
     1741 tccaaaccga cggctccaat catcaccgtt gcgtcggtcc cgccgcttaa gggcatcgct
     1801 gcactgaccg cgctttgtcc attgcgataa acccgagccg ttgagccgtc ataggtaaca
     1861 gtcacctgct cccactcgcc a
//
LOCUS       DAOTBO010000247         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_46808_length_1859_cov_4.570399, whole genome shotgun sequence.
ACCESSION   DAOTBO010000247 DAOTBO010000000
VERSION     DAOTBO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46808_length_1859_cov_4.570399"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLE88_13600"
     CDS             complement(<1..870)
                     /locus_tag="PLE88_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77543.1"
                     /translation="MSLRFQRIASLVWLFLLGIPVVSAPYFDMPYEFEQPDGTKLTLI
                     LNGDEFYMEAQTPDGYTVVRDPQTGWICYAQLSDDGEELVSTGQPVEIQDSDSEQTAA
                     KQKEIAGGRKIEKKLKIKPSSKLKKIRQKQKLLFGDDFQLINGQAYPADSTAAADSIA
                     QPADDTQAAAAGGVLPPTKSISNFTGTIKGLVVIFDFSDAPAGYTLEQYRDKVNKANA
                     YKSNGTAASLRTYYEDVSRGVFIMNHVVYGIFRAPQTFAYYDNLPYTEGARQLLSIAL
                     NQMEAAGFDFSQLT"
     gene            complement(1195..>1859)
                     /locus_tag="PLE88_13605"
     CDS             complement(1195..>1859)
                     /locus_tag="PLE88_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOM77544.1"
                     /translation="VNHWPEDVAAALAAQMPCVSIEHTYRKTKLDVICTDENQGMELL
                     TEHLCTLGHRKIGFFGNCGSVYHARSRFAAYISALAQYDLKLDLSNVIDVLPGHLEDK
                     EIALTGQIESVIRRIHQQGVRAWVCASDWVGYLLCRGLIDRGYNIPKDVSIVGFDDSE
                     DNTLGCPKLTSITVPALRIGAEALRRLLTRIRHPAGPPMKVMLPCKLFEAQTTAAPPK
                     TL"
BASE COUNT          452 a          448 c          452 g          507 t
ORIGIN      
        1 cgtcaattgg ctgaaatcaa agccggcagc ttccatctgg ttgagggcaa tactcagcag
       61 ctgccgggca ccttccgtat aagggagatt atcgtaatag gcaaaggtct ggggcgcccg
      121 aaagatgccg tagaccacat ggttcataat aaaaacgccc cgtgacacat cttcataata
      181 ggtgcgcaaa gaagccgctg tgccgttgct tttataggca ttggccttat tgaccttgtc
      241 ccgatactgt tcgagcgtat agccggccgg agcatcggaa aaatcgaaaa ttaccaccag
      301 acccttgatg gtgccggtga aattgctgat gcttttcgtc ggtggcagaa caccgccggc
      361 agcggccgcc tgcgtatcgt ctgcgggctg ggcaatgctg tcagcggctg ccgtgctgtc
      421 ggccggatat gcctgaccgt tgatgagttg aaagtcgtcg ccgaaaagca gtttctgctt
      481 ctggcggatt tttttcagct tggaagacgg ctttatcttt aatttttttt caatttttct
      541 gccgccggct atctctttct gcttggctgc tgtctgttct gaatcactgt cctgaatttc
      601 aacgggctga ccggtagaaa ccaattcctc gccatcatcg gataattggg catagcaaat
      661 ccatcctgtt tggggatctc tgacgacggt atagccgtca ggtgtttggg cctccatata
      721 aaactcatcg ccgttgagaa taagcgtcag ttttgtgccg tccggctgct caaattcata
      781 cggcatatcg aaatacgggg cagatacaac gggaataccc agtaaaaaca gccatacaag
      841 agatgcaatc ctctgaaatc gtagggacat tcgctataaa tcctttcttt acccgcctgc
      901 aggtatcgta attttatatt tccccatttt aaatacggac atctgtcaag aattggaaaa
      961 aattctcgcc ttattgtgca gattattaaa aagtttaatt cccttaaaaa gaaccttttt
     1021 accggaactt gccttgctgt attgtcagtg attttaaata tcgttctgca aagaggatat
     1081 gttaatcaga tttcaggtgt tttccggcgt tctttgaggg ttattttata taaagacata
     1141 atatctcttt attcagcaac tgtctaagct gagcttgccg gcctattggg tgttttacaa
     1201 tgtttttgga ggagccgctg tcgtctgggc ctcaaagagc ttacagggca gcattacttt
     1261 catcggcggg ccggcaggat gacgaatccg tgtcagaagc cgccgcagcg cttcggcacc
     1321 aatccgaagc gctgggacgg tgatactggt tagtttgggg caaccgagcg tgttgtcttc
     1381 gctgtcgtcg aatccgacaa tcgatacatc tttcgggatg ttataacccc gatcgattag
     1441 tcctcggcaa agcaaataac ccacccaatc actggcgcag acccacgccc gaaccccttg
     1501 ctgatgtatt cgacgaataa cggattcgat ttgtccagtg agggcaattt ctttatcttc
     1561 cagatgtccc ggcaggacat caatgacatt cgataagtcc agtttcaaat catattgtgc
     1621 cagagcactg atgtaggcgg caaagcggct tcgggcatga taaacagaac cgcagtttcc
     1681 gaaaaaaccg attttacgat gccccagggt gcacaaatgc tcggtcagca gctccatccc
     1741 ctgattttca tccgtgcaga tgacatccag ttttgttttg cggtaggtgt gttcaatgga
     1801 aacacagggc atttgggcag ctaaagcagc agccacatcc tccggccagt ggttgacta
//
LOCUS       DAOTBO010000248         1841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_47387_length_1841_cov_3.619261, whole genome shotgun sequence.
ACCESSION   DAOTBO010000248 DAOTBO010000000
VERSION     DAOTBO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47387_length_1841_cov_3.619261"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLE88_13610"
     CDS             <1..580
                     /locus_tag="PLE88_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77545.1"
                     /translation="HIGPNGIEIICGGSGIETLANLNSFQRQRLMEEMEALHNRADVI
                     LIDTGAGIHSAVIGFCMAADHTLVVTTPEPTAMTDAYAMIKVLSSKQYPGQISLLVNM
                     ACSRIEGKKVYRQISDVAARFLGVPVYEAGILCRDDYLLTAVRQRELVVQAFPKADIS
                     RSLEAIGQRLTQGTNKTINKDGFLRKVANWFF"
     gene            719..1507
                     /locus_tag="PLE88_13615"
     CDS             719..1507
                     /locus_tag="PLE88_13615"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOM77546.1"
                     /translation="MKTAQTDKINIEQVWTEFFKTRSEQCRNLLMEHYLHLVKYTAER
                     IWIKLPDKVELDDLFQAGIFGLKDAIEAYDPERGVKFETYCTPRIRGSILDELRSMDW
                     VPRLVRARAHQLERARQTLEAILGRMPTEEELADELNLDKEEFCRLQRDANAIGLISL
                     NNKFNEHEGEKDIREIDIIKDQKSKNPVTEAQKRDLKNLISKGLTRAERLIIILYYYE
                     EMTMKEIGATLDLSESRVSQMHSSIVARLKAQMTSRKKEFVIHS"
     gene            1632..>1841
                     /locus_tag="PLE88_13620"
     CDS             1632..>1841
                     /locus_tag="PLE88_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77547.1"
                     /translation="MTVDTMSILEYGYGDFLNFTKDTMGLTDKTTAILSKLKSIETLK
                     RLCIDADCHIDVQNDISILSEPVQAG"
BASE COUNT          530 a          403 c          455 g          453 t
ORIGIN      
        1 tcatattggt cccaacggca tagaaatcat ctgcggcggg tcgggaatag aaacgttagc
       61 caacctgaat agctttcagc gtcagcgtct gatggaggag atggaagctc ttcacaaccg
      121 agctgatgtg attctaattg ataccggtgc gggtattcat tcggcagtca tcggtttctg
      181 catggcagca gaccatacgc tggtcgtcac cactccggag ccgacagcca tgactgatgc
      241 atatgcgatg attaaggtac tcagctccaa gcagtatccg ggtcagatta gtctgcttgt
      301 caatatggcc tgttcacgca tcgaggggaa aaaagtatat cggcaaatat ccgatgtagc
      361 agctcggttt ctcggtgtgc cggtctatga ggcaggaatc ttgtgtcggg atgactattt
      421 attgacagcc gtacgccaac gggaacttgt agtacaggca tttcccaaag cagatatctc
      481 tcgttcattg gaggccatcg gtcagcgtct gacgcaggga acgaataaaa caattaacaa
      541 ggatggattt ttacgtaaag tggctaactg gttcttttaa agttaaccgc aaaaacggcc
      601 gatagttttt cgcctcattg cggaagtttg gcgccagtag aggcggacgg tcaataagag
      661 acgttaaact tatcggacct ataaatgcac aatactgtaa aacatggaag tttcaggcat
      721 gaaaaccgcc cagacagata aaataaatat cgaacaggtt tggacagagt tttttaagac
      781 ccgctcagag cagtgccgca atctgctgat ggaacattat ctgcatctgg ttaaatatac
      841 tgctgaacgc atctggatta agctgccgga taaagtcgaa ctggatgact tgtttcaggc
      901 cggcatcttt ggtcttaagg acgctatcga agcctacgac ccggaacgcg gggttaagtt
      961 cgaaacgtat tgcactcccc gaatccgcgg cagtatattg gatgagcttc gcagtatgga
     1021 ttgggtaccc cgactggtcc gggccagggc tcaccagctg gagcgagccc gccaaactct
     1081 ggaagccatt ctcggacgaa tgccgacgga agaggaattg gccgatgaat tgaaccttga
     1141 caaagaagaa ttctgccgcc tccagcggga tgccaatgcg ataggattaa tttcgctgaa
     1201 taacaagttt aacgaacatg agggtgaaaa ggatattcgc gaaatcgata ttattaaaga
     1261 ccagaaaagc aaaaatcctg ttacggaagc gcagaaacga gatctgaaaa acctgatttc
     1321 caaaggtctg acccgcgcgg aacggcttat cattattctg tattactatg aagaaatgac
     1381 aatgaaggaa atcggggcaa cgctggactt gtccgagtcg cgtgtttcgc aaatgcattc
     1441 atcaatcgta gcccggctga aagcccagat gaccagccgg aaaaaggaat ttgtcattca
     1501 ttcctgacag aattccgcac gctgctttgc agctctcaaa atagttgata gtaaactgca
     1561 ttgacagttc atcctcccta tgctaattct cttacctgaa tcatttcctc agaagcagat
     1621 taaagtgtca attgacagta gacacaatgt caattttgga gtatggttat ggggatttcc
     1681 tgaattttac aaaggatacg atgggattaa cggataaaac aactgccatt ctgagcaaac
     1741 ttaagtccat agaaaccctt aagcgtctat gcatcgatgc agattgtcat attgatgtac
     1801 agaacgatat ctccattctg tcagagccgg tgcaggccgg t
//
LOCUS       DAOTBO010000249         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_48234_length_1817_cov_3.675369, whole genome shotgun sequence.
ACCESSION   DAOTBO010000249 DAOTBO010000000
VERSION     DAOTBO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48234_length_1817_cov_3.675369"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLE88_13625"
     CDS             <1..188
                     /locus_tag="PLE88_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77548.1"
                     /translation="VDEWKLKAIRPDNHWLDCLVGCGVAASMEGAKLFGTEADRENRP
                     AKIRLSQLQKGKQLCRT"
     gene            176..349
                     /locus_tag="PLE88_13630"
     CDS             176..349
                     /locus_tag="PLE88_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77549.1"
                     /translation="MQDIKVKPSKGLVCKACGGRKFKVIYTRAAHDGRLMRRRQCCRC
                     GRRFTTWESMSCL"
     gene            572..645
                     /locus_tag="PLE88_13635"
     tRNA            572..645
                     /locus_tag="PLE88_13635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Leu,seq:taa)
     gene            660..845
                     /locus_tag="PLE88_13640"
     CDS             660..845
                     /locus_tag="PLE88_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77550.1"
                     /translation="MKNTIQENASGPKRASGDGISVEQHSLADQIAADKYLQSKQAAA
                     AKGLGIKLLKISPGGTT"
     gene            845..>1817
                     /locus_tag="PLE88_13645"
     CDS             845..>1817
                     /locus_tag="PLE88_13645"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOM77551.1"
                     /translation="MWPFKTKTKPAQISLPVTLHAKYDAAQTTRENLRHWAMADGLSA
                     DAAASSDVRKTLRQRARYEVSNNSYAKGITLTLANDCVGTGPRLQVLTGDDAFNRKVE
                     GAFSLWAQRVGLAAKLRTMRMAKATDGETFAILTGNGAIAGPVKLDVHLVEADRVTTP
                     PLKQMDENTIDGIELDSSGNPVRYTILRYHPGDLRATSDDYDTIGADAVIHWFRSDRP
                     GQHRGVPEITPALPLFAQLRRYTLAVLGAAETAADFAAVLFTDAPANGEAASVEPMDV
                     VELEKRMATVLPDGWKLGQIKAEQPATAYGEFKREILNEIARCLNLPY"
BASE COUNT          396 a          509 c          551 g          361 t
ORIGIN      
        1 tggtggatga atggaagctg aaggccatcc gcccggacaa ccactggctg gactgcctgg
       61 tggggtgtgg ggtagcggcc tcgatggaag gagccaaact gttcggaaca gaggccgacc
      121 gggaaaatcg ccctgcgaag attcgacttt cacaactcca gaaaggcaag caactatgca
      181 ggacataaaa gtaaaaccct ccaaaggact ggtctgtaag gcctgtggcg ggcgaaagtt
      241 caaggtcatt tatacccgtg ctgctcacga cggccgtttg atgcgccggc ggcaatgctg
      301 ccgctgcggc aggcgattta cgacgtggga atcgatgtca tgcttgtaaa ccgtaaattt
      361 ttcaccccca atgtctatat acgtaatcat tttggcgggc ctgggttttt catttgaacc
      421 cttccacttt ttgggcataa tcagacgtga caactgaata aggcgttggg cgcgactgat
      481 catcgcagcc gaaacgaaag ctaacaaggc cgtgtggggc cacaccccca tgcggccttt
      541 ttgttttggc cgacgcccgg ttgtcagtag agcgggatag agcaatggca gctcgcgagg
      601 gtttaaaacc catcgaggtt gaaggttcaa gtccttctcc cgccagtatg gctgaagact
      661 tgaaaaatac gattcaggaa aacgcctctg gccccaaacg agccagcgga gatggtatct
      721 ctgtcgagca gcattctctg gccgaccaga ttgccgcgga taagtacctg cagtccaaac
      781 aggccgcagc agccaagggc ctgggaatca aactcctgaa gatttctccg ggagggacga
      841 cctgatgtgg ccgtttaaga ccaaaaccaa gcctgcccag atctcgctgc cggtgacgct
      901 gcatgctaag tacgatgccg cccagaccac ccgtgagaac ctgcggcact gggcgatggc
      961 cgatgggcta tcggcggatg cggcggcctc cagcgatgtc cgcaagaccc tgcgtcagcg
     1021 ggcacggtat gaggtgtcca ataattctta tgccaagggg attaccttaa ccctggccaa
     1081 cgattgcgtg ggtaccggtc cccggctgca ggtcctgacc ggcgatgatg ccttcaatcg
     1141 caaggtcgag ggggccttca gcctgtgggc ccagcgggtg ggattggcgg ccaaattgcg
     1201 taccatgcgg atggctaaag ccaccgatgg agagaccttt gccatcctga cgggcaatgg
     1261 ggctattgcc ggcccggtca agctggatgt ccacctggtg gaggccgacc gggtgaccac
     1321 cccgcccctg aaacagatgg atgaaaacac cattgacggg attgagcttg attcttccgg
     1381 caatccggtg cggtatacca tccttcgcta ccatccaggt gaccttcgag ccacttcgga
     1441 tgattatgat actatcggcg ccgatgcggt cattcactgg ttccggtccg accggcccgg
     1501 ccagcatcga ggcgtgccgg agattacccc tgcgcttccg ctgtttgccc agttgcggcg
     1561 atataccctg gcggtcctgg gggcggccga aaccgcagcg gattttgcgg cggtcctgtt
     1621 cacggatgcc ccggccaatg gcgaggcggc ctcagtcgag ccgatggatg tggtcgagct
     1681 ggaaaaacgc atggccacgg tcctgccgga tggctggaag ctgggccaga tcaaggccga
     1741 gcagccggcc accgcttatg gggaattcaa acgggagatc ttaaatgaga tcgcccgctg
     1801 cctgaacctg ccctaca
//
LOCUS       DAOTBO010000250         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_48809_length_1801_cov_3.749141, whole genome shotgun sequence.
ACCESSION   DAOTBO010000250 DAOTBO010000000
VERSION     DAOTBO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48809_length_1801_cov_3.749141"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1618
                     /locus_tag="PLE88_13650"
     CDS             284..1618
                     /locus_tag="PLE88_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOM77552.1"
                     /translation="MNREWIFPDNWQVLIVALCSVLHQRCAWRLAVLVAGIIFAKGRR
                     TVTSWFRCAGIGRQYKAFYYFIGSMGRKTEIVATILFECMVQLIYKDQSRVLMGIDDT
                     PTPRYGPHVEGAGIHHNPITSPDGAKFVYGHIWVTLSALFRHRRWGTIGLPLLAKMYV
                     RAKDMAKVPKYYHVPFKTKLQQAAELVQWAYTCCKRLQKELWIVTDGGYTKAKFLKPV
                     ISTGINLISRLRADAALCATVKPVKKRKQGRPRMYGQRIYLGQMVADKSGWFSVKVIL
                     YGQEETKRVKLFKAMYHPAGCCVLVLIVREDKNKWRAFMCSNLLATAQQILEAVADRS
                     AIEQNFHDLKEIEGTGQQQVRNFWANIGAFHLNLWVHTMVELWAWDKPAAVISDRSDS
                     PWDNTDRRPSHADRCAGLRREVMAKTFFATSGHSRKTRKIVRQFYKLMKLAA"
BASE COUNT          476 a          441 c          442 g          442 t
ORIGIN      
        1 gattttcccg gcaatcagac cgctgggaaa cgtcagatcg ggaagcgcaa aaaattcttc
       61 taaaattaaa gcggcagcac cgtacaaagc cgtcctttcc ttatacgggc ttaaatgaac
      121 tttaaacccc gaaagtattt tatccatcac catctctttc tgcactttcc aaaaaaaata
      181 acagttaaca ccgctcttta aaatacaaat ttgcaaataa aaatggctca tccttgccga
      241 tgttttgagt agcaaaacaa aaaacagcag gaaaggagag cccatgaatc gcgaatggat
      301 ttttccggac aactggcaag ttctgatcgt ggcgctttgc agtgtccttc atcaacgctg
      361 tgcctggcga ctggcggttc ttgtagccgg aatcatcttt gccaagggac gcagaacggt
      421 gaccagttgg ttccgctgtg ccggcatcgg ccggcagtat aaggcgttct attatttcat
      481 cggctcgatg ggcagaaaaa ctgaaattgt tgcaacaata ttgtttgaat gtatggtaca
      541 gcttatttac aaagatcaga gcagggtttt gatgggaatc gacgataccc ctaccccgcg
      601 gtacggccct catgtggaag gcgctggaat ccatcacaat ccgattacca gcccggatgg
      661 cgccaagttc gtctatggcc atatctgggt cacgctgtcg gccttgttcc gtcatcgacg
      721 ctggggaacc atcggattgc cgctgctggc taaaatgtat gtccgggcca aggatatggc
      781 caaggtgccg aagtattatc atgtaccctt caaaaccaaa ctccaacagg cggccgaact
      841 ggtgcaatgg gcttatactt gctgtaaacg gctgcaaaaa gaactttgga ttgtcaccga
      901 cggcggttac actaaagcga aatttctgaa acccgtcatc agcacaggga taaaccttat
      961 cagccgtttg cgggccgatg ccgccctgtg tgcgacggtt aaaccagtta agaagcgcaa
     1021 gcaaggccga cctcgcatgt atggtcagcg tatctatctt ggtcaaatgg ttgccgacaa
     1081 atccggttgg ttttcggtca aggtaatcct ttatgggcaa gaggaaacca aacgggttaa
     1141 actgtttaag gcgatgtatc atccggccgg atgctgtgtt cttgtgctga ttgtccgtga
     1201 agacaagaac aagtggcggg cgtttatgtg cagcaatctc ttggctaccg cccagcagat
     1261 tcttgaggcg gttgcagatc gttccgcaat cgaacagaat tttcacgacc tgaaggaaat
     1321 cgagggtacc ggccagcagc aggtccgaaa cttttgggcc aatatcggag cgttccattt
     1381 gaatctgtgg gttcatacga tggttgaact gtgggcgtgg gacaaacccg cggcggtaat
     1441 ctctgaccgc agcgacagcc cgtgggacaa cacggaccgc cgcccgtctc atgcggaccg
     1501 gtgtgccggc cttcgcagag aagtaatggc taaaacattt tttgcaactt cggggcatag
     1561 ccgtaaaact cgaaaaatcg tgcggcagtt ctataagctc atgaaactgg ccgcttaact
     1621 atttattttt tggaaagtgc agtctctttg aaaaccctgt taaattcatc aaacaggatt
     1681 tgacccgcca catcaatttt accgcacaca acaagctcat gcggattcag cgtctgtatt
     1741 aaattggctg tcgccatcgc cagataccgt acaatctccc gaacaatcat ttcacaatct
     1801 t
//
LOCUS       DAOTBO010000251         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_49048_length_1794_cov_3.960897, whole genome shotgun sequence.
ACCESSION   DAOTBO010000251 DAOTBO010000000
VERSION     DAOTBO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49048_length_1794_cov_3.960897"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /locus_tag="PLE88_13655"
     CDS             <1..>1794
                     /locus_tag="PLE88_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOM77553.1"
                     /translation="YRETLFQGSTEDVRVDAAVGLLLQNNAQSREVLVSALKAEENPL
                     ARQSVCKALIKSRGLSQTISAKEIFLEPLIGILQGKSPEQAELAAEAMLLFDYAEIAG
                     PIRQLLDNKELSTQTRGNAIYALQVRPEPLALRSLIGLLDNPDADIAKSAETALQEAF
                     GIPVGTSRSVWADILSELQRKSPEDIRRERLLRQEMKLREVQAESARWQKLYIGSLDK
                     QYETLDEAGRGKMLLELMDSDLAPIRLWALDKLTKYPIGTNEKLREKLFSFLSDSSRD
                     VRLQTAKALMNMSVLNPAARLLERFSQEKDPEVALAMFGALGEAVFFAFSSNSQIELS
                     PEIKTRTLAIATEYLNQDAADAAEKGATVIGKILELNNLPPESMQSYLTALKDRYNRS
                     ALQSSALRANLLAILARLCGQGGSKEAARVLYEPLFKEALKVTDNPGLRLAAAQGLVY
                     IDKVKALELFRQNQLMLDESIAVRQIVIDLAGQTGQGADLEWLLKMLSGNGYSEQVWL
                     AIRSICQRQSAAFLLEWAGSIKAAEGLTISYVREIMDMAEQKAAAEKDSELLLKIQQA
                     SISWIAEQKMWEQGGSYLTKINYSAAENGFS"
BASE COUNT          478 a          391 c          481 g          444 t
ORIGIN      
        1 tttaccgcga aactctgttt cagggcagca cagaagatgt ccgtgtggat gctgctgttg
       61 gcctgcttct tcaaaataat gcacaaagcc gagaagttct tgtctctgcc ttgaaagcag
      121 aagaaaatcc tcttgcccgt cagtccgtat gcaaggcatt gattaaaagc cgcggattga
      181 gccagaccat cagtgccaaa gagatattcc tcgagccgct gatcggaatt ttgcagggca
      241 agtcgcctga acaggcggaa ttagctgcgg aagcgatgct gcttttcgat tatgctgaaa
      301 tcgcaggacc aatcaggcag ctccttgata ataaagaact tagcacacag actcgaggca
      361 atgccatata tgctctgcag gtccggcccg aaccgcttgc tttgcgcagc ttaatcggac
      421 ttctggataa tccagatgcc gacatagcaa aatcagccga aacggcccta caagaggcat
      481 ttggcatccc ggttggcacc agccggtcgg tttgggctga tattttaagt gagcttcagc
      541 gaaaaagtcc cgaagatata agacgcgaac gtctgcttcg tcaggaaatg aagctgcggg
      601 aagtgcaggc tgaaagtgcc cgctggcaaa agctctatat aggatcgctg gataagcagt
      661 acgagactct ggatgaggcc gggcggggca aaatgctgct ggaattgatg gattcggatt
      721 tggcgcccat tcggctgtgg gcactggata agctgaccaa atacccaatc ggcaccaacg
      781 aaaagcttcg cgaaaaactg ttttcttttt tatcggattc ttcgcgagac gtacgcttgc
      841 agacagccaa agctctgatg aatatgagtg tgctgaatcc tgcggcaaga ttgctggaac
      901 gttttagtca ggaaaaagac ccggaagtag cgctggcaat gtttggggcg ctgggggaag
      961 cggtgttttt tgctttttcg agcaattcac aaatagaatt gtcgccggaa ataaagaccc
     1021 gcacactggc cattgccact gaatatctta atcaggatgc ggcggatgcg gctgaaaagg
     1081 gagcgactgt tatcggtaag atattggaat taaataactt gcctccagaa tcaatgcagt
     1141 cctatttgac agctcttaag gatcgctata atcgctctgc tttacaaagt tcggctttac
     1201 gggcaaattt attggctatt ttggcccgtc tttgcggaca aggcggttca aaagaagcgg
     1261 ctcgcgtcct atatgaaccg ttatttaaag aggccttgaa ggttacagat aatcccggtt
     1321 tgcgtttagc cgcagcacaa gggcttgtct atatcgataa ggtcaaagcc cttgaattat
     1381 tcaggcaaaa tcagctgatg ctggatgaaa gcattgcagt tcggcagatt gttattgatt
     1441 tggccggaca gacaggccaa ggtgcggatt tggagtggct tttgaaaatg ctgtccggca
     1501 atggctacag cgaacaggtt tggctggcta tccgctctat ctgccagcgt caatcagctg
     1561 cctttttgct ggagtgggca ggttcaatta aagccgcgga gggtttgact atcagttatg
     1621 ttcgcgagat tatggatatg gccgaacaaa aagcggccgc agaaaaagat tctgaacttc
     1681 tattaaaaat tcagcaggca tccatttctt ggatagctga gcagaaaatg tgggagcaag
     1741 gcggcagtta tctgacaaaa atcaactact ctgccgcaga gaatggtttt tcgg
//
LOCUS       DAOTBO010000252         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_50464_length_1755_cov_3.780588, whole genome shotgun sequence.
ACCESSION   DAOTBO010000252 DAOTBO010000000
VERSION     DAOTBO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50464_length_1755_cov_3.780588"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1755
                     /locus_tag="PLE88_13660"
     CDS             <1..>1755
                     /locus_tag="PLE88_13660"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOM77554.1"
                     /translation="GRGDLAERVQLTTVRYLAHPYILHTESGWNEVKSKIDAYPWAQQ
                     ALTDYSKQASVWQVPAVHSDTGYVYPLAEAQRAVACAVAWKLTGSRAWEAKAVAWIRQ
                     FADPDKGYLKTQRACNAGRVHEGLFFQEFAKTYDLLYDSTLLTSADHQQIEKTLRKFL
                     EIADWDLQAGDGNNHQISFCAGALLCSLTLQDFERAERFLNGPHGLRDLLASGILDDG
                     SYFEQAPNYNILVANIAMMVVQACRPWGLGLDRWTIEPRYSRTILLAPWINDNQFLGM
                     SFDRYGPSRRNYRTIKDLWDSLVPLADYRGVLFAGNDSAEKDLASDHQEAAPGLELAY
                     YLYRDPNYIPIIRNAKKRNLLYGVPNLPQTDWSPWAFSVCQDNSGFAVLRSQPETMQP
                     RQRIQAVLKFGTHGGYHGHFDRASLMSLMRYGRSFYSTEAAWYGYDSYLFKMWVQASV
                     SHNMVVVDQRMQKPAEGKLLLFYPGRQIQAAAAETSSQWIDPPYGGQTPYRESFPQEK
                     SWKESRYLPIPDVPRPQGDTGLPSEPVLQRRLMVVTQDYVIVADFLKGSEEHTFDNLI
                     HIKGLKDFHAAAKVFRNHT"
BASE COUNT          412 a          454 c          476 g          413 t
ORIGIN      
        1 cggcagagga gacttggctg agcgtgtcca gctgacaacg gtccgctatt tggctcatcc
       61 ttatattttg cataccgagt ctggctggaa tgaggttaaa tccaaaatcg atgcctatcc
      121 ttgggcacag caggcactga cggactatag caaacaggca tccgtctggc aggtgccggc
      181 tgttcattcc gataccggct atgtttatcc tcttgctgaa gcccagcggg ctgttgcctg
      241 tgcggttgct tggaaactga ccggcagcag ggcatgggag gcaaaagcgg tcgcatggat
      301 ccgccaattt gccgatcccg acaaggggta tctgaagaca cagagggcct gtaacgccgg
      361 acgcgtccat gaaggtcttt tctttcagga atttgccaaa acttatgacc ttctctatga
      421 cagtacgctg ctgacttctg ccgaccatca gcagatagaa aagaccctgc ggaaatttct
      481 ggaaattgcc gattgggatt tacaggccgg cgacggcaat aaccaccaga tatctttttg
      541 cgcaggggcg ctgctgtgca gtctgacact tcaggatttc gaacgcgctg aacgcttctt
      601 gaacggcccc catggcctcc gcgatctgct ggcaagcggc attctggatg acggatcgta
      661 ttttgagcag gcccccaact acaatatcct tgtagccaat atcgcaatga tggtagtaca
      721 agcctgccgg ccgtggggat tggggttgga cagatggact atcgaaccgc gatacagccg
      781 tacaatattg ctggctccat ggatcaatga caatcagttt cttggaatgt cgtttgacag
      841 atacggacct tcccgccgaa attaccggac aatcaaggat ttatgggatt cactggttcc
      901 gctggccgac taccgcggcg ttctttttgc cggcaacgac tctgctgaaa aggacttggc
      961 ttcggaccat caggaagccg cgcccggtct ggagctggcc tattatctct accgtgatcc
     1021 gaattacatc cctatcattc gaaatgcaaa aaagcgaaat ctgctctatg gggtgccgaa
     1081 tctgccccag acagattgga gcccgtgggc tttctcggtg tgtcaggaca attccggctt
     1141 tgccgtgctg cgatcccagc cggaaactat gcagccccgt cagcggattc aggcggttct
     1201 gaagttcggc acacatggcg gctatcacgg ccattttgac cgggccagct taatgagtct
     1261 gatgcggtac ggcaggagct tttacagcac agaagccgct tggtacggct acgattccta
     1321 tctgtttaaa atgtgggttc aggcctctgt gtcgcacaat atggtggttg tcgaccagcg
     1381 gatgcagaag ccggctgaag gaaaactgct gctattttat cccggccgcc aaatccaggc
     1441 agcggcggcg gaaacctcta gtcaatggat agatccccct tatggcgggc agacacccta
     1501 tcgagagtca tttccgcagg aaaaaagctg gaaagaatcc cgttatctcc ccatacccga
     1561 tgtgccgcgg ccgcagggcg atacgggctt gcccagcgaa ccggttctcc agcggcggct
     1621 gatggtggtt acgcaagatt atgtgatagt ggccgatttt ttaaaaggaa gtgaggaaca
     1681 tacatttgac aacctgattc atattaaagg actaaaagat tttcatgccg cagcaaaagt
     1741 atttcgcaat catac
//
LOCUS       DAOTBO010000253         1644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_55003_length_1644_cov_2.434235, whole genome shotgun sequence.
ACCESSION   DAOTBO010000253 DAOTBO010000000
VERSION     DAOTBO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55003_length_1644_cov_2.434235"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..>1644
                     /locus_tag="PLE88_13665"
     CDS             413..>1644
                     /locus_tag="PLE88_13665"
                     /inference="COORDINATES: protein motif:HMM:NF033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOM77555.1"
                     /translation="MKPRIAKIVRRRKRKLQKRLAKTSQPAQPGPMLNPGAIEYDAAE
                     RSAGMVYGGIGAVHLLIKQLKLDEAINAQLNIFKLYNPYTESDHVLNIALNILCNGDC
                     LEDIERLRNDAVYLDAVGAARIPDPTTAGDFCRRFTSWHVEMLQDIINAVRLKVWGQQ
                     KPDFFKEALIDADGTIAPTTGQCKEGMDLSYKGLWGYHPLLISLANTGEPLFLVNRSG
                     NSTSSKDAASYLDQAAALCRQGGFETVRFRGDTDFSQTAYLDQWDSEGISFVFGIAAM
                     ANLINLAETLPETAWKLLSRKPRYAVATEPRRKPAKVKEQIVQERGYKNIRLESEQVA
                     EFDYRPVKCKQAYRMVVVRKNLSVEQGQAVLFDDIRYFFYITNDRKTRPEEIVRHAND
                     RCNQENLIAQLKNGVSAMR"
BASE COUNT          439 a          422 c          430 g          353 t
ORIGIN      
        1 aagaacccga tcagccgaat attgaaagga tctatgaaat aattgagttt ttacaggact
       61 gtctaacgga cattcggtca ctgaaacgaa aataattgac ttaatgaacc aataaattta
      121 taatagcgga aaggagaaat aaaatgaaac gattacttcc ttgtttttgt atattggtaa
      181 cttgcatatc cttctcttca ggggcttggg attttcaatt gacaaatgga tattatggaa
      241 gccatactct gacaggttct cagcgattac taatccttaa acaagcgtac cctgaataat
      301 aaaaaatgtt aacaattcac aaaaatactc gaaatggccc ttagagtctg ttatattgca
      361 tttgttaaat aacgacgcaa acaacaagaa ctcttgaaag gagccatttc gagtgaagcc
      421 aaggatagcc aaaatcgtgc ggcgacgcaa gcgaaaactt caaaagcggc tggccaaaac
      481 atcgcagccc gcacagcccg gcccgatgct caatcccggg gcgattgagt acgatgcggc
      541 cgagcgcagc gccgggatgg tgtatggcgg cattggcgcg gtgcacctgc tgatcaagca
      601 gttgaagctg gatgaagcga tcaatgcaca gctgaacatt ttcaagctct acaatccgta
      661 caccgaatcg gatcatgtgc tcaatatcgc cctcaatatc ctgtgcaacg gcgactgcct
      721 ggaggacatt gagcggctgc gaaacgacgc ggtctatctg gatgccgtcg gcgcagcgcg
      781 gattcccgat ccgaccaccg cgggcgactt ttgccgccgc ttcacttcct ggcacgtcga
      841 gatgctgcag gacatcatca acgccgtccg cctgaaggtc tggggacagc agaagcccga
      901 ctttttcaag gaagccctga ttgacgccga cgggaccatt gccccaacaa ccggccagtg
      961 caaggaaggg atggatttat cctacaaggg gctctggggg tatcatccgc tgctcatttc
     1021 cctggccaac accggcgaac cgctgtttct ggtcaaccgc agcggcaaca gcaccagcag
     1081 caaggatgcg gcgagttatc tggatcaggc cgccgccctg tgccgtcagg ggggctttga
     1141 gacggttcga tttcgaggcg atacggattt cagtcagacc gcctatctgg atcaatggga
     1201 cagcgaaggc atttcctttg tcttcgggat tgcggcgatg gcgaatctga tcaacctcgc
     1261 cgaaaccctg ccggaaacgg cctggaagct gctttcgaga aaaccgcgtt atgccgtggc
     1321 caccgaaccc cgccgcaagc cggccaaggt caaggaacag attgtccagg aacgcggcta
     1381 caaaaatatt cgacttgagt ccgaacaggt ggccgagttt gactaccgtc ccgtcaaatg
     1441 caagcaggcc taccggatgg tggtggtccg caagaatctg tcggtcgaac agggccaagc
     1501 ggtattgttt gacgacattc gctacttttt ctacatcacc aatgaccgca agacgcggcc
     1561 tgaagagatt gtccgtcatg ccaatgaccg gtgcaatcag gagaatctga ttgcccagct
     1621 gaaaaacgga gtcagtgcta tgcg
//
LOCUS       DAOTBO010000254         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_55427_length_1635_cov_2.667089, whole genome shotgun sequence.
ACCESSION   DAOTBO010000254 DAOTBO010000000
VERSION     DAOTBO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55427_length_1635_cov_2.667089"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLE88_13670"
     CDS             complement(<1..964)
                     /locus_tag="PLE88_13670"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOM77556.1"
                     /translation="MQIVMNLIYCIALVLYSPKIIYRRLFENRYREGWRQRRGFIDRR
                     CPDKPCIWIHAVSVGEVNAARTLIDELRRQMPNYEVVLSSTTDTGLARAKSQYEKDLS
                     VFYFPLDFSWTMRRAFDRLRPALILLMELEVWPNMAAIAAKRGIPVVVVNGRLSDKSF
                     PRYKKIRWLVQRMFGKVAFVLAQTEEYARRFIELGCQPHKVIVTSSLKYDTAQTEGPV
                     CGADRLAQQIRLDKEPLWVAGGTGPGEEKIALNVFAKLRQDSRLAGLRLAIIPRKPER
                     FDEVAALIEQVDFAIASYRGAIVIALPLNAFELQRLSRLGIERGE"
     gene            complement(1274..>1635)
                     /locus_tag="PLE88_13675"
     CDS             complement(1274..>1635)
                     /locus_tag="PLE88_13675"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOM77557.1"
                     /translation="NNKFLNGKLDKMIWLLWAELRVNGDVDALQTPTGWIPRYEDLVP
                     LFADKLGKKYTQDDYVKQFTVRVANLLDKFDRVEKIYRDKVPDTPQIVYDTFAAIRKR
                     LQEARSRYGDFISPLAL"
BASE COUNT          387 a          460 c          422 g          366 t
ORIGIN      
        1 gctcacctcg ctcgatccca agacgggaaa gtcgctgaag ttcgaacgca ttcaagggca
       61 gggcaattac tatagctccc cggtaggagg cgatggcaaa gtctacctgc tcaatcaacg
      121 cggcgacttc gtcgaaccgt tcgggttttc gcgggatgat tgccagccgg aggccagcaa
      181 gccggctgtc ctgccggagt ttggcaaaga cattcaaagc aattttttcc tcgcccggcc
      241 ctgttcctcc ggcaacccac agcggctcct tgtcaaggcg aatctgctgg gccagacgat
      301 ccgccccgca gacaggacct tcggtctggg cagtgtcata tttgagcgaa ctggttacga
      361 taactttgtg cggctgacag cccagttcga tgaaccggcg ggcatactct tccgtctggg
      421 ccagcacaaa agcgaccttc ccgaacatcc gctgcacaag ccagcggatt ttcttatagc
      481 gggggaaact cttgtcgctg agccggccgt ttacaaccac tacaggaatc ccgcgcttgg
      541 ctgcaattgc agccatatta ggccacacct ccagctccat cagcaaaatc agtgccggcc
      601 gaagacggtc aaaagcccgc cgcattgtcc atgaaaaatc caacggaaaa taaaataccg
      661 acaggtcctt ttcatactgc gatttggcgc gtgccagacc ggtatcggtc gtcgaggaaa
      721 gtacgacttc gtaattgggc atttgacggc gcaattcgtc gatgagcgtg cgggcggcat
      781 tgacctcgcc gacactgacg gcatgaatcc aaatgcacgg tttgtcgggg catcggcggt
      841 caataaatcc gcgccgctgc cgccagccct cgcggtagcg attctcaaac aagcggcgat
      901 agataatctt ggggctgtac agcaccagcg caatgcagta aatcaggttc atcactattt
      961 gcataagaaa atcatatctg ctaatgatgc ggctgtcaaa cattcaaagg cattcaaagc
     1021 agtaaggtca ttgagctgtc ttacggcttt tgggtgtgca tccggcgctg ccgaaaactc
     1081 ctgtaaacag cgtgctgtta agccgttgtt ttataccgcc tcgatcggga tctattggag
     1141 ccatccgaac ggattcatcc gccaaattga tgtccgctgt caggataaaa cctatcccgt
     1201 aatcattcgg ctattcgcag cgacaaagaa aacccggcac gcctcaaggg ccttttggca
     1261 tccgaactta cggttacagg gccagcgggc tgatgaaatc gccatatctg ctgcgggcct
     1321 cctgcagccg cttgcggata gccgcaaagg tatcatagac aatctgcgga gtgtccggga
     1381 ctttgtcgcg ataaattttt tctacgcgat caaacttatc cagcagattg gcaacccgga
     1441 ccgtaaactg cttgacataa tcgtcctgcg tgtacttctt gccaagcttg tctgcaaaca
     1501 gcggcaccaa atcttcatag cggggtatcc agccggtagg cgtctggagg gcatcaacat
     1561 caccattgac ccgcagttcg gcccacagca gccaaatcat cttgtccagc ttgccgttga
     1621 ggaatttgtt gtttt
//
LOCUS       DAOTBO010000255         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_56870_length_1603_cov_2.476744, whole genome shotgun sequence.
ACCESSION   DAOTBO010000255 DAOTBO010000000
VERSION     DAOTBO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56870_length_1603_cov_2.476744"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PLE88_13680"
     CDS             <1..1333
                     /locus_tag="PLE88_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77558.1"
                     /translation="LGGSIWAATVQIPADVSCRTDINNRTANRHDSNKLSIRGDASAA
                     KSWIKWTDLNGIDVKTIRKAQLRLTTYETRPGMFDVSAVNDDYQTNINWAEREITWDN
                     AAANATESYTAVLAAAATYVGRMDFRNTVAGDQRFIDVTSILKADTDGIVQFILHNST
                     TLMNMCTHDQGGTMIDGTVIGPEAARPMLFITLPPAGADLPNPEIDQVVPNTLAALSW
                     TNPEPNIPGTLITCEVFLGTEPNLLTMDRKTLAAGATSAAINTTNFPTYGALANNTRY
                     YWKVDCYDPSRQPALIPGEMWSFFVGQPPSVNAGPDQSVWLTDGSVTVNLDGTVSDDG
                     PFTVAWTQVSTGAPTVTISPDNVQDTSITLTARGTYEFMLTADDGTLQSSDTVRVVVG
                     NTPCDASHLASGKPYNTADANQDCIVDLTDFAALIIQDWLECTDTLMLCAR"
BASE COUNT          386 a          414 c          460 g          343 t
ORIGIN      
        1 gcttggcggc agtatttggg cagcgacagt gcagattccg gcggatgtga gctgtcggac
       61 cgacattaac aatcgtaccg caaaccggca tgacagcaac aaactgagca tccgggggga
      121 tgcctcagcg gccaagtcat ggattaaatg gaccgatctg aacggcatcg atgtcaaaac
      181 gattcggaaa gcgcagctgc gtctgacgac ttacgagaca cgccccggca tgttcgatgt
      241 atcggctgtc aacgatgact atcagaccaa tatcaattgg gccgaacggg agattacatg
      301 ggataatgcc gcggccaatg caaccgaaag ctatacagcc gtgcttgcag cggcggctac
      361 ttatgtcggc cggatggact ttcgcaacac ggtagcaggc gaccagcgct ttatcgatgt
      421 gacatcgatt ctcaaagcgg ataccgatgg aattgttcag ttcatcctgc acaattcgac
      481 gactctgatg aatatgtgca cccatgacca gggcggcaca atgatagatg gaaccgtgat
      541 aggtcctgag gcagcacggc ctatgctgtt tattaccctg ccgccggcag gagccgactt
      601 gccgaatccg gaaattgatc aggttgtacc caatacgctg gccgctttgt cgtggaccaa
      661 tcccgagccc aatattccgg gaaccctgat tacctgtgag gtatttctgg gtacagaacc
      721 caatctgtta acaatggata ggaaaactct ggcggccggg gcgacgtcag cggccatcaa
      781 tacaaccaat ttccccacct atggggcatt ggccaacaat acccgctatt actggaaggt
      841 ggactgctac gatcccagtc ggcagccagc cttgattccc ggtgaaatgt ggtcgttttt
      901 tgtcggccag ccgcccagtg tgaatgcagg gccggaccag tctgtatggc tgactgacgg
      961 tagcgtaacg gttaatctgg acggcaccgt tagcgatgat gggccgttta cagtcgcttg
     1021 gacgcaggtg tccaccggtg cgccgacggt tacaatcagc ccggataatg ttcaggatac
     1081 gtctataacc cttaccgcac ggggtacgta tgaatttatg ctgacggctg acgacggcac
     1141 gctgcagagt tccgatacgg ttcgggtggt agtcggcaac acgccctgcg atgcatcgca
     1201 tctggcatcc ggcaaaccct ataatacggc ggatgccaat caggactgca ttgtcgattt
     1261 gaccgatttt gccgcgctga ttatccaaga ttggctggaa tgcacggata cgctgatgct
     1321 ttgtgcacga taatagaaga atcaaggggt tcgggcctgc gggcattgtc cggcggcctg
     1381 aactcttcga aaagccaaga cgccggctgt gtgccccaag cagccgaata aagacggaag
     1441 gcaggccgaa aaatgaaaca caggaaaaac agaatcggtt ttacgctgat tgaactgctg
     1501 gtggtgattg cgattattgc gctgctgctg tcggtgctgc tgccgggcct gaagaaagcc
     1561 aaggattact ccaagcgggt tatctgcggt gcccggctca agc
//
LOCUS       DAOTBO010000256         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_57776_length_1583_cov_4.142670, whole genome shotgun sequence.
ACCESSION   DAOTBO010000256 DAOTBO010000000
VERSION     DAOTBO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57776_length_1583_cov_4.142670"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="rfbC"
                     /locus_tag="PLE88_13685"
     CDS             complement(<1..456)
                     /gene="rfbC"
                     /locus_tag="PLE88_13685"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOM77559.1"
                     /translation="MKVIQTAIEGVVILEPQMFADHRGFFMETWNRQRYAQAGVDADF
                     VQDNLSFSHRGVLRGLHFQHPQGQGKLVQVLEGEVFDAAVDIRRGSPTFGQAVTCRLS
                     ADNHRQFYVPAGFAHGFCVLSQTALFSYKCTDFYQPHYEGGILWNDPSLG"
     gene            complement(453..1334)
                     /gene="rfbA"
                     /locus_tag="PLE88_13690"
     CDS             complement(453..1334)
                     /gene="rfbA"
                     /locus_tag="PLE88_13690"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOM77560.1"
                     /translation="MNRKGIILAGGSGTRLYPATQAVSKQLLPVYDKPMVYYPLSVLM
                     LAGIRDILVISTPHDLPQYRMLLGDGSQWGLRLEYAVQPRPEGLAQAFLIGRTFLGGC
                     PSCLILGDNIFYGHGLTEALQQAIARPSGATIFGYYVRDPERYGVIELDSSGAVAGIE
                     EKPAQPKSNYAAVGLYFYDAQAPALAAQLKPSARGELEITDLNRAYLRQGQLYVQLLG
                     RGIAWLDTGTPDAMLQAGNFVQIVQQRQGLMICCPEEIAYRQGWIDEEQVLALAAGLN
                     KNHYGQYLLRLVGGPQE"
     gene            complement(1388..>1583)
                     /locus_tag="PLE88_13695"
     CDS             complement(1388..>1583)
                     /locus_tag="PLE88_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77561.1"
                     /translation="SYKTKHYTYRRLHFKKKAIDYPNKTAILPISQIKIDYYRIQKQK
                     SLCIAAKPPAGFGCCISIWI"
BASE COUNT          354 a          485 c          403 g          341 t
ORIGIN      
        1 tcccagcgac gggtcattcc ataagatgcc gccttcgtaa tggggctggt aaaagtccgt
       61 gcatttgtac gaaaacaagg ccgtctggct caacacgcaa aagccgtggg caaagccggc
      121 gggaacataa aactgccggt ggttgtccgc tgaaaggcgg caggtaaccg cctgaccaaa
      181 ggttggactg ccccggcgga tatccaccgc cgcatcaaag acctcgcctt ccagcacctg
      241 caccagcttg ccctgcccct gcggatgctg gaagtgcagc ccgcgcagca cgccccggtg
      301 cgaaaacgac agattatcct gaacaaagtc cgcatccaca cccgcctgtg catagcgctg
      361 gcggttccac gtttccataa agaagccgcg atggtccgca aacatctgcg gctccagaat
      421 caccaccccc tcgatagccg tctggataac cttcattcct gcggcccccc gacaagccgc
      481 agcaggtatt ggccgtagtg atttttattc agccctgctg ccagcgccag cacttgttcc
      541 tcatcaatcc agccctgacg gtaggcaatc tcttcaggac agcaaatcat cagcccctgc
      601 cgctgctgaa ctatctggac aaaattgccc gcctgcagca ttgcatcggg cgtgcctgta
      661 tccagccaag caatgcccct gcccagcaat tgcacataaa gctgtccctg ccgcagatat
      721 gcacggttca aatcggtaat ttccagctcc ccgcgtgccg agggcttcag ttgggcagcc
      781 aaagccggcg cttgggcatc ataaaaatac agccccaccg ccgcataatt ggattttggc
      841 tgagccggct tttcctcaat gccggccacc gctccgctgc tgtccagctc aataacgccg
      901 tagcgttcgg ggtcgcggac ataatagccg aatatcgtcg ctcccgacgg ccgggcgatg
      961 gcctgctgca gggcttcggt cagcccgtgc ccatagaaaa tgttgtctcc cagaatcagg
     1021 caggatggac agccgcccag aaatgttcgt ccaatcagga atgcctgtgc cagaccttcc
     1081 ggccgcggct gtacggcata ctccaagcgc agtccccact ggcttccgtc gcccagcagc
     1141 atgcggtact ggggcaggtc gtgcggagtc gaaatcacca gaatgtcccg aatgccggcc
     1201 agcatcaata cgctcagcgg ataatacacc atcggcttgt catagaccgg cagaagctgc
     1261 ttgctgacgg cctgcgtggc cggatacagc cgcgttccgc tgccgccggc cagaatgatt
     1321 ccttttcggt tcatagcgtt ccttttctct aagacggccg caattgtaag agaaaaaacc
     1381 tgcagggtca aatccaaata ctgatgcagc atccaaagcc ggctggcggc ttggccgcta
     1441 tacagagact tttttgcttt tgtatccggt aataatctat ctttatttgc gaaataggca
     1501 aaattgcggt tttatttgga taatctatcg cttttttctt gaaatgcaga cgccgatagg
     1561 tatagtgttt tgttttataa ctc
//
LOCUS       DAOTBO010000257         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_58530_length_1568_cov_4.729676, whole genome shotgun sequence.
ACCESSION   DAOTBO010000257 DAOTBO010000000
VERSION     DAOTBO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58530_length_1568_cov_4.729676"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="PLE88_13700"
     CDS             complement(<1..1559)
                     /locus_tag="PLE88_13700"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOM77562.1"
                     /translation="MVKTILEKSIDPINLKKLTAVSNPKLHAFIAEAIELLKPSKVLV
                     VTDSKEDLANLREMAKNGGGEIPLAIPGHTYHFDGPNDQGRDTFSTRYLLPKDMELGE
                     SLNSIDRQQGLDEIRGLLKNAMKGRTMIVCFWCLCPTGSDFAISCVQITDSPYVAHSE
                     SILYRPGYEQFKKLGPNDEFFRFMHLEGQTDENGCSIHWEKRRVYIDLIDNTVYSVNT
                     QYAGNTVGLKKLALRLAIQKASKEGWLAEHMFIMGAKGPGGRTTYFAGAFPSACGKTS
                     TAMLPGQTIIGDDIAYFRKRGGRVYAANVEQGIFGIIGDVNPDDDPVIFKALQSPGEV
                     IFGNVLIADGKPYWEGMGCRLPERGINFQGQWYPGKKDGDGKEIPPSHKNARYTIRLD
                     ALANLDPELENPQGVPVGGIIYGGRDSDTLVPVEQAFNWTEGIIAKGASIESETTAAT
                     LGQHGVRKFNIMANQDFVSIPLGRYIQNNLDFAKGVKNPPAIFSVNYFLRDENNKFLN
                     GKLDKMIWLLWA"
BASE COUNT          354 a          411 c          413 g          390 t
ORIGIN      
        1 gcccacagca gccaaatcat cttgtccagc ttgccgttga ggaatttgtt gttttcatcc
       61 cggaggaaat aattgaccga gaaaatcgcc ggcggatttt taacgccttt ggcaaaatca
      121 aggttgttct ggatgtagcg gcctaaagga atcgatacaa agtcctgatt ggccataatg
      181 ttgaatttgc gcacgccgtg ctggcctaat gtggccgccg tggtttcgga ttcgatcgag
      241 gcccctttgg caatgatgcc ctcggtccag ttgaaggcct gctcgacagg aacaagggta
      301 tcgctgtcgc gtccgccgta aataatcccg ccgacgggca ccccttgcgg attttccagc
      361 tccggatcca gattggccag cgcatccaga cggattgtgt agcgggcatt tttatgcgag
      421 ggcggtattt cttttccatc gccgtctttt ttgccagggt accattggcc ctgaaaattg
      481 atgcctcgtt caggcagcct gcagcccatt ccttcccaat agggcttgcc gtcggcaatc
      541 aagacgttgc cgaaaatcac ttcgccgggg ctctgcaggg ccttgaaaat aaccggatca
      601 tcatcgggat tgacatcgcc gataatgccg aagatgccct gttcgacatt ggcggcatag
      661 accctgccgc cgcgtttgcg gaaataggca atgtcatcgc cgataatggt ctgaccgggc
      721 agcatggccg tggaggtctt tccgcaggca gagggaaacg ctccggcaaa ataagttgtg
      781 cggccgccgg gtcctttggc gcccataata aacatatgct cggccagcca gccctctttg
      841 gaggccttct ggatagccag ccgcagcgcc agttttttca gccccaccgt gttgccggcg
      901 tactgcgtat tgaccgaata gaccgtattg tcaatcaaat cgatatagac ccgccgcttc
      961 tcccaatgga tgctgcagcc gttctcatcg gtttgccctt ccaagtgcat aaaacggaaa
     1021 aactcgtcat tggggcccag ctttttgaac tgctcgtagc cgggacggta gagaatgctt
     1081 tcgctgtggg caacataagg ggaatcggtt atttggacgc acgaaatcgc aaaatcggaa
     1141 ccggtggggc acaagcacca gaagcagaca atcatggtac gtcccttcat ggcatttttg
     1201 agaagcccgc ggatttcgtc caaaccctgc tgacggtcga tggaattgag cgattcgcca
     1261 agctccatat ctttgggcag caggtagcgg gtgctgaagg tatcgcggcc ctgatcattg
     1321 ggaccatcaa aatgataggt atggccggga atggcaagag gaatctcgcc gccgccgttt
     1381 ttggccattt cacgcagatt cgccaaatcc tctttcgaat ctgtaacaac caatactttt
     1441 gaaggtttca aaagctcgat cgcttcggca ataaatgcat gaagcttcgg attgcttaca
     1501 gcagtcagct ttttaagatt tatcgggtct atactttttt ctaaaatagt tttgaccacg
     1561 ccttgttc
//
LOCUS       DAOTBO010000258         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_61911_length_1504_cov_5.212560, whole genome shotgun sequence.
ACCESSION   DAOTBO010000258 DAOTBO010000000
VERSION     DAOTBO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61911_length_1504_cov_5.212560"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLE88_13705"
     CDS             <1..452
                     /locus_tag="PLE88_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOM77563.1"
                     /translation="IAVGIPFEELTRCYKAANPAMAAKMGFKKAGQFVLRSYVTLGRL
                     FQRSVPVSSLAGPVGILSMSYQVAGDSLAHYLYFLGLISSCLAVMNLLPLPVLDGGHI
                     VFLLIEKITGRPIHERVLAPIMYAGMALLLGLILWVTYHDILRIMFG"
     gene            445..1173
                     /locus_tag="PLE88_13710"
     CDS             445..1173
                     /locus_tag="PLE88_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOM77564.1"
                     /translation="MDRNLGTLNRTFRKLWYFGRSRYCPVCGSWVRKFNSYGRACRSD
                     AQCPVCQSLERHRFVWIYFKQRTCLFDGQPRRMLHFAPEPEFTRQFRKIRTLEYISAD
                     LMNPDVSLKTDICQIDCPDESFDIVYCSHVLEHVQDDCKAIREIYRVLKKNGLAVILV
                     PISAKATFEDPSVTDPRLREQMFGQHDHVRQYGPDFLERLAAQHFSAAAVYVSELLSP
                     QQAEIMGLKAIDERKMPVFSCQKL"
     gene            1199..>1504
                     /locus_tag="PLE88_13715"
     CDS             1199..>1504
                     /locus_tag="PLE88_13715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOM77565.1"
                     /translation="MGITRAVSMHDKRSISVVIPAYNCQAYIRRAIDSVLGQSRPADE
                     IIVVDDGSTDGTAEAVRTYGAKVILIQQENAGVSAARNAGIRAASGDWIAFLDADDEW
                     "
BASE COUNT          340 a          356 c          412 g          396 t
ORIGIN      
        1 tgattgccgt cggcattccc tttgaggaac tgacgcgctg ctacaaggcc gccaatcctg
       61 caatggcggc aaaaatgggt ttcaaaaagg ccggtcagtt tgtcctgcgc agctatgtga
      121 cgctcggacg gctgtttcag cgcagcgttc cggtctcctc cttagctggt ccggtcggca
      181 ttctttcgat gtcttatcag gtcgccggtg attcactggc tcattacctc tattttctag
      241 gcctgatcag ctcttgtctg gcagtgatga acctattgcc tctgccggtt ctcgacggcg
      301 ggcatattgt gtttctgctg attgaaaaga taaccggccg gcctatccat gagcgcgtgc
      361 tggcgccgat tatgtatgca ggcatggcat tgctgctggg cctgattctg tgggtgacat
      421 atcatgatat cctgcggatt atgtttggat aggaatcttg ggacattgaa tcggacgttt
      481 cgaaaactat ggtatttcgg gcgaagccgc tattgtccag tctgcggcag ctgggtaaga
      541 aagtttaatt cctatggccg ggcatgccgt agcgatgctc aatgtccggt ctgtcagtct
      601 ttagagcgtc atcgtttcgt atggatttat ttcaaacagc ggacctgcct gttcgacggc
      661 cagcccagac ggatgcttca ctttgccccc gagccggaat tcacacgaca gttcagaaaa
      721 atccggacgc tggaatacat atcagcggat ttaatgaatc cggatgtctc attgaaaacc
      781 gatatttgcc agattgactg tcctgacgag tcgtttgata ttgtctattg cagccatgtt
      841 ctggagcatg ttcaggatga ttgcaaggcc ataagggaaa tctatcgggt gctcaaaaaa
      901 aatggactgg cagtcattct ggtgccaatt tcggcaaagg cgacatttga agacccttct
      961 gtaaccgacc ctcgtctgcg cgaacagatg tttgggcaac atgaccatgt ccgtcagtat
     1021 gggcctgatt ttctggaacg tttggctgcg cagcattttt ctgccgcggc ggtttatgta
     1081 tcagagcttc tttctccgca gcaagccgaa ataatgggat taaaagcgat cgatgaaaga
     1141 aaaatgccgg ttttttcttg ccaaaaactg taacaattca aggggtactg tgtaaaggat
     1201 gggaataacg cgggccgttt cgatgcatga caaaagaagc atcagtgttg taatccctgc
     1261 atataattgc caagcgtata tcaggcgggc aattgacagc gttttaggcc aaagccggcc
     1321 ggcggatgaa atcatcgtcg ttgacgacgg ctccaccgac ggcacagccg aagcggtccg
     1381 aacctacggg gccaaagtga ttttgattca gcaggagaat gccggcgtca gcgccgcccg
     1441 aaatgccggc atccgtgcgg catccggtga ttggattgct tttctggatg ccgatgatga
     1501 gtgg
//
LOCUS       DAOTBO010000259         1487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_62895_length_1487_cov_5.016760, whole genome shotgun sequence.
ACCESSION   DAOTBO010000259 DAOTBO010000000
VERSION     DAOTBO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62895_length_1487_cov_5.016760"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..>1487
                     /locus_tag="PLE88_13720"
     CDS             147..>1487
                     /locus_tag="PLE88_13720"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOM77566.1"
                     /translation="MTMTGSAKLKEFLIFCFVLSPIVQGQPDSGQTPSAGHSIVFRRF
                     EYAGRDLNADTPLQPGYYRNPILAGFHPDPSICRVGSDYYLVNSTFEYFPGLPIFHST
                     DLVNWTQLGHVIDRPEQLDYRNRRISGGLYAPAITYYKGLFYVVCTMVDGPGNFVVTA
                     ENPAGPWSNPTPLGFAGIDPSLFFDEDGRAWIVNNDAPQGPPRYSGHRAIWIQEFDYK
                     AKKMIGPRTMLVDGGVDISTKPVWIEGPHIYKRKDWYYLCCAEGGTGPDHSQVIFRSR
                     KVDGPYQPWEKNPILTQRNLSADVPGAVTCTGHADLEIGPDGNWWAVFLAVRPYANGL
                     SPMGRETFLLPATWTEDDWPIILPAGQRVPLTAKAPGGAAAAPSPSAAFNGSFTWVDQ
                     FRQETLSPEWLMLRQPKEKWWTLNAQAGTLALTARQERLTGSGNPSYLGRRVRHP"
BASE COUNT          308 a          407 c          420 g          352 t
ORIGIN      
        1 cggggactgc cggattgggc tggatgaatt tgctgtgttt gcggctgact ggctgaatgc
       61 atcctttcaa tgacgcctac cggcagtaat atttgcaaat ttgtaaaaaa ccggtataat
      121 gacaacttta aacaacggag gtttatatga caatgacagg cagcgccaaa ctgaaagaat
      181 tcctgatttt ctgcttcgtc ctttccccga ttgtccaagg ccaacccgac agcggacaaa
      241 ccccttctgc cggccactcg attgtctttc ggcggtttga atatgccggc agggatttga
      301 atgccgatac cccgctgcag ccgggctatt accgcaatcc gattttagcc ggttttcatc
      361 ctgatcccag tatctgccgc gtcggaagcg actattattt ggttaattcg acatttgagt
      421 actttccggg tcttcccatc tttcacagca cggacttggt gaactggacc cagctgggac
      481 atgtaattga ccgccccgag cagctggact acagaaaccg ccgcattagc ggcgggctct
      541 atgcgccggc gattacctat tacaagggtt tgttttatgt tgtctgcaca atggttgacg
      601 gtcccggcaa ttttgtggtt acggccgaaa atcctgccgg cccgtggtcc aatccgacgc
      661 ctctgggctt tgccggaatc gacccttccc tcttttttga tgaggatgga cgcgcttgga
      721 ttgtcaacaa cgatgccccg cagggtccgc cgcgctacag cggtcatcgt gccatctgga
      781 ttcaggaatt tgattacaag gccaaaaaaa tgatcggtcc gcgcacgatg ctggttgacg
      841 gcggggtgga tatttcgacc aagcccgttt ggatcgaagg gccccatatt tacaagcgca
      901 aagactggta ttatctctgc tgtgccgaag ggggcaccgg acccgatcat tcacaggtca
      961 ttttccgcag ccggaaagtc gatggtcctt atcagccgtg ggagaaaaat ccgatcctga
     1021 cgcagcggaa tttaagcgcc gatgtgcccg gtgcggttac ctgtacaggc catgccgatt
     1081 tggagatagg tcccgatgga aactggtggg cggtttttct ggctgtgcgt ccgtatgcaa
     1141 acggtttatc gccgatggga cgggaaacct ttctgctgcc ggcgacatgg acagaggatg
     1201 actggccgat cattcttccg gccggccagc gtgtgccgtt gacagcaaaa gcccccggcg
     1261 gggctgcagc tgctccctct ccgtcggctg cttttaacgg cagcttcacg tgggtggacc
     1321 agttcaggca ggagacgctt tcgccggagt ggctgatgct gcgccagccg aaggaaaaat
     1381 ggtggactct gaatgctcag gccggcacgc tcgcactgac agcccgtcag gagagactga
     1441 ccggttcggg caaccccagc tatctgggcc gccgcgtccg ccaccct
//
LOCUS       DAOTBO010000260         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_62967_length_1486_cov_3.199860, whole genome shotgun sequence.
ACCESSION   DAOTBO010000260 DAOTBO010000000
VERSION     DAOTBO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62967_length_1486_cov_3.199860"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLE88_13725"
     CDS             <1..883
                     /locus_tag="PLE88_13725"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOM77567.1"
                     /translation="ESLMDKLNDPFTLDELTAQIEKERHESHRRSQTALVTRDGMIWL
                     AKSNYELRFLPEQRISQRIIFPTGPSEQNGIEDARFVQFKNDDGSQIYYATYTAYDGK
                     TILPQLMQTEDFLNFKIITLNGKAVQNKGMALFPRKLNDKYVMLSRQDNENLFIMFSD
                     NIHFWYEMTPLLKPTYPWEFVQVGNCGPPIETKEGWLVLTHGVGPMRKYCIGAILLDL
                     EDPRKVIGRLNQPLLMPGANEREGYVPNVVYTCGALIHQGNLILPYAMSDYASGMAIV
                     ELEPLLELLKTGQSRSV"
     gene            1240..>1486
                     /locus_tag="PLE88_13730"
     CDS             1240..>1486
                     /locus_tag="PLE88_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOM77568.1"
                     /translation="MNSSLSRIIRQYNADPARLMDILLDCQSELGCISGEFRKQIASE
                     LNISEVDVEQTMSFYHFLSSEPRGKYTVYLNNSVVAKM"
BASE COUNT          420 a          341 c          316 g          409 t
ORIGIN      
        1 cgaatcgctg atggataagc tcaatgatcc cttcacatta gatgaattga cagctcagat
       61 agaaaaagag agacatgaaa gtcacaggcg ttcccagaca gctcttgtta cccgtgatgg
      121 aatgatctgg cttgccaaat ccaattacga actccgtttt ctgcctgaac agcggatttc
      181 ccaaagaatc attttcccca cagggccaag cgagcagaac ggcatcgagg acgcaagatt
      241 tgtgcagttt aaaaatgacg atggaagtca gatctattat gccacctaca cagcatacga
      301 tgggaaaaca atactgcccc agttgatgca gactgaggat tttctcaatt tcaagataat
      361 cacgcttaac ggtaaagcgg ttcagaataa aggaatggcc ctgtttccca gaaaactcaa
      421 tgataaatac gtcatgctct ccagacagga taatgaaaac ctgtttataa tgttttctga
      481 taatatccat ttctggtatg aaatgactcc gcttttaaaa cccacttatc cctgggagtt
      541 tgtgcaagtc ggcaactgtg gaccacccat cgagacaaaa gaaggatggc ttgtactcac
      601 tcatggggtg ggacctatgc gtaaatactg tataggtgcg attcttctgg atctcgaaga
      661 ccccagaaaa gtgataggac gtctcaacca gccgcttctt atgccaggtg ctaacgagcg
      721 cgaagggtac gtccccaatg tggtttacac ttgcggggca ttaatccatc agggaaacct
      781 gattctcccc tatgcgatgt cggattatgc atccggaatg gcaatcgtgg aactggagcc
      841 tcttctggag ttgctaaaaa ccggccagag cagatcggtg tgaggccttc atggaataaa
      901 agcccggttt aaccgggctt ttatttagcc agaggcggca ttgatgccgc cgaaaaatgc
      961 ggcaaacgcc gccttatccc ttgggaaacc agtctcctct caaacccgca atatcctcag
     1021 ccccgtttcc gtagttctta tcggtttttg tgaaatttgg ttcaatatta tgcggaaatt
     1081 ccaattatat tcccgttttt cgattacttg attattattt ttttgttcaa aaccgttcat
     1141 ttatttattt tatgttatgg aatgtccata tatatatttt tcaattaaga ttcttgaact
     1201 cggtcccgat acacctcaac cagaaaaggt gaaacggtta tgaactcctc tctttccagg
     1261 atcataaggc aatacaatgc tgaccctgct cgtctgatgg acattcttct ggattgtcaa
     1321 tcagaactgg gttgcatctc cggtgagttc agaaaacaaa tcgcctccga acttaatatc
     1381 tccgaggtag acgtcgaaca gaccatgtct ttttaccact ttctctcctc agaaccaaga
     1441 ggaaagtaca ctgtttacct caataacagt gtagtggcta agatgt
//
LOCUS       DAOTBO010000261         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_63316_length_1480_cov_3.254035, whole genome shotgun sequence.
ACCESSION   DAOTBO010000261 DAOTBO010000000
VERSION     DAOTBO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63316_length_1480_cov_3.254035"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLE88_13735"
     CDS             <1..652
                     /locus_tag="PLE88_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOM77569.1"
                     /translation="FNLLVMILHSHSAVTAAAITIVFNWDMVSFVPMIGINVGVTSLV
                     GRYMGAGLPDTAHRATLSGLKLAWMYSSCILLMFAFFPDCLVGLFRPRHETALFAAAY
                     PMAVTMLRMAALYVMADAVMLVFGGALRGAGDTFWAMCISVVLHWILVALLFVFVRMW
                     DVPPAASWLTLCCTFMLLSMLFYLRYRQGKWRTLRLIGQEFSPPAAVNGFRETKDL"
     gene            689..>1480
                     /locus_tag="PLE88_13740"
     CDS             689..>1480
                     /locus_tag="PLE88_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOM77570.1"
                     /translation="MKTKPYTADSRRYDKMIYNRCGRSGLKLPAISLGLWHNFGDVDD
                     FGAARQMILAAFDNGITHFDLANNYGPPPGSAETNFGKILKKDLAPYRDELIISTKAG
                     YLMWPGPYGEWGSKKYLIASLEQSLKRMGLEYVDIFYSHRPDPDTPLEETMEALAQAV
                     HQGKALYAGISNYSPQQTRRAAAVLAQMGIRLLIHQPPFSMLRRTFETAGLFETLEEL
                     GIGAICFSPLAQVLLTDKYLQGIPADSRAAKPHGYLKKESVTPDLI"
BASE COUNT          323 a          368 c          372 g          417 t
ORIGIN      
        1 attcaatctg cttgtaatga ttctgcattc tcacagcgct gtaactgctg ctgcaatcac
       61 cattgttttc aactgggata tggtttcttt tgtgccgatg atcggcatta acgtcggcgt
      121 taccagtctg gttggacgct atatgggtgc agggctgcct gatacggccc atcgcgcaac
      181 cttgtcagga ctgaagctgg cttggatgta ctccagctgc attctgctga tgtttgcatt
      241 ctttccggac tgtctggtcg gcctattccg tccccggcac gaaaccgccc tgttcgctgc
      301 tgcttatcct atggcagtca ccatgctgcg gatggctgcc ctttatgtaa tggccgatgc
      361 tgttatgctt gttttcggcg gagcgctgcg gggagccggc gacaccttct gggcaatgtg
      421 catctctgtt gttctgcatt ggatattggt tgctcttcta tttgtctttg tcagaatgtg
      481 ggatgtaccg ccggccgcat catggctgac gctgtgctgt acatttatgc tgttaagcat
      541 gctgttttat cttcggtatc gtcagggcaa atggcgcaca ctgcgtctta tcggacagga
      601 attctctcct cccgcagccg taaacggatt tcgtgagaca aaagatttat agttttcaaa
      661 acagcatgct tgatgtacaa taaacagtat gaaaacaaaa ccctatacgg ctgacagccg
      721 ccgttatgac aaaatgattt acaaccgctg cgggcgaagc ggactgaagc ttcctgccat
      781 ctctttgggg ttatggcata attttgggga tgtcgatgat ttcggtgctg cccggcagat
      841 gatacttgct gcgtttgata atgggattac ccactttgac ttggcaaaca attacggccc
      901 tcctcccggc agtgccgaga cgaatttcgg caaaatcctt aaaaaagatt tggcccccta
      961 ccgtgatgaa ctgattattt ccaccaaagc cggctatttg atgtggccgg gtccttatgg
     1021 agaatggggg tcgaaaaaat atcttatcgc cagtttggag cagtccctca agcggatggg
     1081 attagaatat gtggatattt tttattcgca tcgccccgat cctgatacgc cactagaaga
     1141 gaccatggaa gcattggcgc aggctgttca tcagggaaaa gccctgtatg caggcatttc
     1201 caattattcc ccgcagcaaa cccgacgtgc cgccgctgtg cttgctcaga tgggaattcg
     1261 cctgcttatt catcagccgc ctttctcgat gttgcgccga acttttgaaa cagccggatt
     1321 atttgagacc cttgaagaat tgggaatcgg tgccatctgt ttcagcccgc tggctcaagt
     1381 acttcttaca gacaagtatc tgcagggtat tccagccgat tcacgggccg ccaaacccca
     1441 tgggtattta aagaaagagt ccgttacacc ggacctgatc
//
LOCUS       DAOTBO010000262         1453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_64923_length_1453_cov_3.860515, whole genome shotgun sequence.
ACCESSION   DAOTBO010000262 DAOTBO010000000
VERSION     DAOTBO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64923_length_1453_cov_3.860515"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLE88_13745"
     CDS             <1..910
                     /locus_tag="PLE88_13745"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOM77571.1"
                     /translation="LKGKTMLMAGKTDRVNMEVSQGNARKISGENGKISNIRCQSDHF
                     VRVINSESRCVWRGQNLDLADIVGGGNGLGTGKRGSCIDTTTGEWKPESYLPSSSEDF
                     KPGTHMKSNYCFHAVKDNPFIDGVFIPDNGQGPVVISTQGHSFEGFPDTSELGWGGIV
                     YVEESILKHPIKLNNVQYGVPERSALFMHGNAGITFDLEQIRQAFPGSLREFRAVYGI
                     ADDYWDGVGCPAYADFWVLVDGQVRFSRKGVQVHQGGTISVVLSDQDRFLTLVTTDGG
                     KGSPEYDNRTSFNDWSVFGEPCLIFD"
     gene            1120..>1453
                     /locus_tag="PLE88_13750"
     CDS             1120..>1453
                     /locus_tag="PLE88_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77572.1"
                     /translation="MTDYKNRFPDPFNGNYEVLWVGPLRPYYQDGGEAMRMCPSATKE
                     EFTPPGWTHAWQISPTGASEDYFKSSYGVNNYVYDHKDPDGDGLLWGREISFHWKRSD
                     VKGASQIPL"
BASE COUNT          390 a          299 c          389 g          375 t
ORIGIN      
        1 ccttaagggc aagacgatgc tgatggccgg caagaccgat agagtaaata tggaagtcag
       61 ccagggaaat gccaggaaga tctctggtga aaacggaaag atttcaaata tccgctgcca
      121 gtccgatcac tttgttcgcg ttatcaactc cgaatcgcgc tgtgtctgga gaggacaaaa
      181 tttagacttg gcggatattg tcggcggcgg caacggctta gggacaggca aacggggctc
      241 ctgtatcgat acaacaaccg gcgaatggaa gccggaaagt tatttgccgt cctcatcaga
      301 agattttaaa cccggcaccc atatgaaaag taattactgt ttccatgcgg taaaggataa
      361 cccgtttatc gatggggttt ttattccgga caacggccaa ggacctgtgg ttatcagtac
      421 acaggggcac tcgttcgaag gatttccaga cacctccgaa cttggttggg gtggaatcgt
      481 ttatgtggaa gagtctatcc tgaagcatcc aattaaatta aacaatgtac aatatggcgt
      541 ccccgaaagg tcggctttat ttatgcacgg caatgcaggg attacctttg atctggagca
      601 aatccgccag gcctttcctg gctctcttcg agagtttagg gcagtgtacg gaatcgcaga
      661 tgattattgg gatggcgtag gatgccctgc ctatgcagac ttctgggtgc tggtagatgg
      721 acaggtgcgt ttttcgagga aaggggtaca ggttcatcag ggagggacga tttccgttgt
      781 attatctgac caggatcggt tcttaacatt ggtgaccaca gatggcggca aaggcagtcc
      841 tgaatatgat aaccgaactt catttaatga ctggtctgtt ttcggagaac cctgtctgat
      901 ttttgactaa ttccagaaag gaatcagcgt atgaaatcga ggttcaaaag atggttttgc
      961 agaaaagcgt ttactttgat tgaactgctg gttgtaattg caatcatatc gcttttgctg
     1021 gccatcctcg ttccttcctt aaaaaaggcc aaggagcaga cacgaaatat catttgccga
     1081 tcgaatttga aacagtgggg agctatctgg agtttgtatt tgactgatta taaaaaccgc
     1141 tttcccgacc cgttcaatgg gaactacgag gttttgtggg tggggccgct gcggccgtac
     1201 tatcaggatg gcggagaggc catgcgtatg tgtccgtcag ccacaaaaga agaatttact
     1261 ccgccgggat ggacacatgc ttggcaaatt agtcccaccg gagcatccga agattatttc
     1321 aaaagcagtt acggtgtcaa taattatgta tatgaccaca aagatccgga cggcgatggc
     1381 ctcttgtggg gacgtgagat tagttttcac tggaaaagaa gcgatgtcaa aggagccagt
     1441 cagattccat tat
//
LOCUS       DAOTBO010000263         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_76822_length_1287_cov_2.627435, whole genome shotgun sequence.
ACCESSION   DAOTBO010000263 DAOTBO010000000
VERSION     DAOTBO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76822_length_1287_cov_2.627435"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1227
                     /locus_tag="PLE88_13755"
     CDS             49..1227
                     /locus_tag="PLE88_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77573.1"
                     /translation="MKICSALFSAAMMLWMLPAGGLGFQAQPVYPSGQMQQFPDVDNG
                     LVVWQQYVQFGSSWDWDIFARDLLDAAAPVPIEAAWLESDQQHPAVWNRSIVWQHAYA
                     PDDWDIYLTDVSNPAEPVWYLLNAQPGQYENNQIAPAVHGNTVVWQHQYIDPDTQQAD
                     WDIWAADVTDPEAPYVFYPASFWKNQQAPAVWRSTVVWQDDDAGRWDIGIADIWLKNA
                     PQDRLYAYPELDQQTPAVWDNWLVFAVDYGGGDWDIHAADLSKPGQPSFPLTDLPSAQ
                     INPDISGHLVVWQDNRNGNWDIYGYNLITRQEFAITDDPADQIHPAVSGTLVVWEDYR
                     VSPSNIYYVWLEGPAAADCPEPPEGDLDGDCRISLTDFVRLAENWMLCARVPASACDL
                     "
BASE COUNT          256 a          382 c          389 g          260 t
ORIGIN      
        1 ttggaacagg aagacgccca gcagtaagcg caggaaacgg aggccgtgat gaaaatctgc
       61 tctgctcttt tttcagcagc catgatgctg tggatgctgc cggccggggg attgggcttt
      121 caagcccagc cggtttaccc ctccggccag atgcagcagt ttcccgatgt cgataacggg
      181 ctggtcgtct ggcagcagta tgtccagttc ggcagcagct gggactggga tatcttcgcc
      241 agagacctgc tggatgccgc tgcgccggta ccgattgagg cggcttggct ggagagcgac
      301 cagcagcatc cggccgtctg gaaccgctcg attgtctggc agcatgccta cgcccccgat
      361 gactgggata tttatctgac cgatgtatcc aatccggccg aaccggtttg gtatctgctc
      421 aatgcccagc cgggacaata tgaaaacaat caaatcgctc cggccgttca cggcaatacc
      481 gtcgtctggc agcaccagta catcgacccc gacacgcagc aggccgactg ggatatctgg
      541 gcggctgatg taaccgaccc cgaagcgccg tatgtgtttt atccggccag cttctggaag
      601 aatcagcagg ccccggctgt ctggcgcagc acggtggtct ggcaggatga tgatgcgggc
      661 aggtgggata tcggcattgc agatatctgg cttaaaaacg ccccgcagga tcgcctgtat
      721 gcctaccccg agctggacca gcagacgccg gcggtctggg ataactggct ggtttttgcc
      781 gtcgattacg gcggcggcga ttgggacatc cacgccgctg acctttccaa gcccggccag
      841 ccctccttcc cgctgacaga ccttccttcg gcacaaatca atccggatat ttcgggccat
      901 ctggtcgtct ggcaggacaa ccgaaacggc aattgggata tctacggcta taacctgata
      961 acgcggcagg agtttgccat aaccgatgac cctgccgacc aaatccatcc ggctgtcagc
     1021 ggcacactgg tagtctggga agattaccgg gtcagcccgt ccaacatcta ttatgtctgg
     1081 ctggaaggcc cggcggccgc cgattgtccc gagccgccgg aaggcgactt ggacggcgac
     1141 tgccggatca gtctgactga ctttgtccgg ctggcggaaa actggatgct ctgcgcacgg
     1201 gtcccggctt cggcctgcga cctgtgaggg ctgccggagc aggcggttta aataaacaga
     1261 ttcaccccgg ctgcttcggc ctgcgac
//
LOCUS       DAOTBO010000264         1188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_85960_length_1188_cov_3.413063, whole genome shotgun sequence.
ACCESSION   DAOTBO010000264 DAOTBO010000000
VERSION     DAOTBO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85960_length_1188_cov_3.413063"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..452)
                     /gene="cas2"
                     /locus_tag="PLE88_13760"
     CDS             complement(123..452)
                     /gene="cas2"
                     /locus_tag="PLE88_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOM77574.1"
                     /translation="MNISGYRAVWIFAMFDLPTDTKKARKDYSDFRKNLLKDGFSMVQ
                     YSVYVRHCASEENADVHYKRVKSFLPPDGEVRLVTITDKQFERMQIFWGKMRKPTPPA
                     PRQLELF"
     gene            complement(449..>1188)
                     /gene="cas1"
                     /locus_tag="PLE88_13765"
     CDS             complement(449..>1188)
                     /gene="cas1"
                     /locus_tag="PLE88_13765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOM77575.1"
                     /translation="THSVLTALLAQGAAVVLCDGTHHPAGMLFALESNTVQTERFRQQ
                     LQAGQPLKKRLWQQLVKAKIAHQASIVADDQQTYRALKALEKQVRSGDPDNIEAQASR
                     QYWTSFLQHIEFKRRRDGAPPNNLLNYGYMVLRAAVARALCSAGLLPTLGIHHRNRYN
                     AFCLADDIVEPFRGFAEKKVKAVCEKYSEIDPLTQELKAQLLEILYQEIQIGDFSGPL
                     MVGLHRTAASLQRCFAGQQKHLELPKV"
BASE COUNT          277 a          306 c          321 g          284 t
ORIGIN      
        1 ttacaatggt ttatggcaac ggacaacgac acaaaaagtt gtggcttgct ctcaggcatg
       61 gattagttac aatacctcat tcataagtcc ttacagagca agcaattgaa aagaattggc
      121 tgctaaaaaa gctcgagctg acgcggcgca gggggtgtcg gcttgcgcat ttttccccaa
      181 aaaatctgca tgcgttcgaa ctgcttatcg gtaatcgtaa ccagccggac ttcgccatcc
      241 ggcggcagaa acgatttgac cctcttgtaa tggacatctg cgttttcctc gctggcacaa
      301 tgccgtacat acactgaata ttgtaccatc gaaaagccgt cttttaacag attttttcga
      361 aaatccgaat aatccttccg tgcctttttg gtatccgtcg gaaggtcaaa cattgcaaag
      421 atccacacgg ctctgtaccc gctgatgttc atactttcgg cagttccaga tgtttctgct
      481 ggccggcaaa gcaccgctgg agggaggcgg ctgtgcgatg caggccgacc atcagcggac
      541 cagaaaaatc gccgatttga atctcctggt aaagaatctc aagaagctgc gctttgagct
      601 cttgggttaa tgggtcgatt tcggagtact tttcacaaac agccttgacc tttttttccg
      661 caaatccgcg aaacggctcg acgatatcat cggccaggca gaaggcattg tagcggtttc
      721 gatggtggat gcccagcgtc ggcagaagcc ccgccgaaca aagcgctctt gcaacggcgg
      781 cccgcaggac catatagccg tagttgagca ggttattggg gggtgcaccg tcgcgtctgc
      841 gcttaaactc aatatgctgc agaaaagaag tccaatactg ccggctggcc tgcgcttcga
      901 tattgtcggg gtcgccggaa cggacctgct tttccaaggc ctttaaggcc cgataagtct
      961 gctggtcatc ggcgacaata gaggcctgat gggcaatttt tgcctttaca agctgctgcc
     1021 agagccgctt tttgagcggc tggccggctt ggagctgctg gcggaaccgc tcggtttgga
     1081 cggtgttgct ttcgagggca aacagcatcc ctgcggggtg atgcgtgccg tcgcacagca
     1141 caacagctgc cccctgggcc aaaagagctg tcaggaccga atgggtat
//
LOCUS       DAOTBO010000265         1065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Anaerohalosphaeraceae bacterium isolate AMR_MDS_2005
            NODE_100447_length_1065_cov_3.859406, whole genome shotgun
            sequence.
ACCESSION   DAOTBO010000265 DAOTBO010000000
VERSION     DAOTBO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864072
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerohalosphaeraceae bacterium (wastewater metagenome)
  ORGANISM  Anaerohalosphaeraceae bacterium
            Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
            Anaerohalosphaeraceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:37:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,755
            CDSs (total)                      :: 2,706
            Genes (coding)                    :: 2,697
            CDSs (with protein)               :: 2,697
            Genes (RNA)                       :: 49
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Anaerohalosphaeraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100447_length_1065_cov_3.859406"
                     /isolate="AMR_MDS_2005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2873829"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..731
                     /locus_tag="PLE88_13770"
     CDS             165..731
                     /locus_tag="PLE88_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77576.1"
                     /translation="MWNIFENSWLLLTAAGIALIAAGIIRQSKPEWGCRALLIPLLLA
                     VLAFGLDAAVTTDYEAIVRIIRSCRQAAVKADASAIMRFVSPRYTDSIHRDKAALAEA
                     AEHILKKASIKKVRIQSHIITTEQPAAQSRLLAAVHLHPNSAYASAGSLVFVELNLEY
                     EKIGKTWSIRRAEVAAINYTPFKWHDAR"
     gene            complement(774..>1065)
                     /locus_tag="PLE88_13775"
     CDS             complement(774..>1065)
                     /locus_tag="PLE88_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOM77577.1"
                     /translation="GLRTRYWMDDGWSINVNYIYNDAIHTNAANSPSDGTAMFHRLDI
                     NVARAFAGQRGEIMVGVADVLNATREPVYDVNSFTSYETPGRMFFVRFQYKF"
BASE COUNT          256 a          297 c          281 g          231 t
ORIGIN      
        1 gggggcatcg cgacccgaaa ggcagcggct ggactgatac aatcgcactg gcggaagggt
       61 atattacgct gacctgtctt cggaaagacc tgaccgaccc ggacggcaat cggctgctcg
      121 ggcaaaaaca gctcctgtac ccttgacacg ctgagaccgg caaaatgtgg aatatatttg
      181 aaaacagctg gcttctgttg accgctgccg gcattgctct tatcgccgcc ggcatcatcc
      241 gtcagtctaa accggaatgg ggctgcagag ccctgctgat tcccctcctg ctggcggttc
      301 tggcattcgg actggatgcc gccgtaacga ccgattacga agcaattgtt cgtataatcc
      361 gttcctgtcg ccaggccgct gtgaaggcgg atgcttcggc aattatgcgt tttgtcagcc
      421 cccgctacac agacagcatt catcgggata aagccgctct ggcggaagcc gcagagcata
      481 tcctcaaaaa ggcctcgatt aaaaaggttc ggatacaatc gcacattatc acaacagaac
      541 agccagccgc ccaaagccgg cttttggcag ctgtgcatct ccatcccaac agcgcctacg
      601 cctctgccgg aagtttggtc tttgtggagt tgaacttgga gtacgaaaaa atcggcaaga
      661 catggtctat tcggcgggcc gaagtggcag cgatcaacta cacgcctttc aagtggcacg
      721 atgcccgctg attatatgcc atgcggatca gcgccgcttt gacagacggg tttttagaat
      781 ttgtactgga atcggacaaa aaacatccgt ccgggcgtct cgtaagaggt aaacgaattg
      841 acgtcataga ccggctcgcg cgtcgcgtta agcacatcgg cgaccccaac cataatttca
      901 ccccgctgcc cggcaaaggc acgcgcgaca ttgatatcca gccggtgaaa catagccgtt
      961 ccatcggatg ggctgttggc tgcattggta tgaatggcat cgttgtaaat atagttgaca
     1021 ttgatcgacc atccatcatc catccaatag cgggtccgca atcct
//
